LOCUS       DATDEU010000001         2391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_145980, whole genome shotgun sequence.
ACCESSION   DATDEU010000001 DATDEU010000000
VERSION     DATDEU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145980"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /gene="fliO"
                     /locus_tag="VIV63_00005"
     CDS             complement(<1..382)
                     /gene="fliO"
                     /locus_tag="VIV63_00005"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY6123002.1"
                     /translation="MDAPLFAAPAVSTAPSSGGGLVEVTLALLAIVALIAGLAWLMKR
                     MRGFGIAGHDRIQVLSERALGPKERCVLVRVGATDMLIGVAAGNVRTLHIFPEGANTE
                     APPAPPAAVPGVPTFKDLLKRSLGK"
     gene            complement(386..691)
                     /gene="fliN"
                     /locus_tag="VIV63_00010"
     CDS             complement(386..691)
                     /gene="fliN"
                     /locus_tag="VIV63_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY6123003.1"
                     /translation="MNAKSEAVVADGESQDVNLEVILDVPVTLSMEVGRTRIPIRNLL
                     QLNQGSVVELDRAAGEPLDVFVNGTLVAHGEVVVVNEKFGIRITDVVSTAERIRKLK"
     gene            complement(696..1679)
                     /gene="fliM"
                     /locus_tag="VIV63_00015"
     CDS             complement(696..1679)
                     /gene="fliM"
                     /locus_tag="VIV63_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY6123004.1"
                     /translation="MAGQEVLNQDEIDALLNGVDQGAVSTDPAPNPGEARQYDFSREM
                     RIVRGRMPTLEMVNERLARQLRVSIYNLLRRQVEIAVGQVSMKKFSEYTHSLSLPVNL
                     NLVKVHPLRGTALFVIDPKLVFAMVDNFFGGVGRQAKIEGREFSSVEMRVVHMFLRSA
                     FTDMKEAWTPIAKIDVEYVNSEINPNFAGIVTPSEVVVVCPFQIDLDGGGGSLHVVMP
                     YSMIEPLREVLDSGVQSDRAEQDDRWLTMLKDGLQDAEVELTTVLGNGSVALSKLVDL
                     KPGDVLPCDFTGKATVLAEGVPLFKGAYGVSRGQQCVRYETKYRRNQNNSR"
     gene            complement(1689..2207)
                     /locus_tag="VIV63_00020"
     CDS             complement(1689..2207)
                     /locus_tag="VIV63_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY6123005.1"
                     /translation="MAQPAATDETLEEGAETAPAKGGKKGLIIGLVLLLVLGGGGFAA
                     FKLLGGKEDPKKKEDAKKEQLLPARYVTLDPPFVVNFEAESSVRFLQITIGIMTRDLE
                     IEKIIKDNDPRIRNDLLLILGGQNYESVSKVEGKEALRTRCLEAVRTVVVESGGDGPK
                     VEALYFTSFVMQ"
BASE COUNT          412 a          744 c          758 g          477 t
ORIGIN      
        1 attttccaag gctccgcttg agcagatcct tgaacgtcgg cacgccaggc acagcggccg
       61 gcggtgcggg cggtgcttcc gtattcgcgc cttcagggaa gatgtgcagt gtgcgcacgt
      121 tgccggcggc aacgccgatg agcatgtccg tggcgccgac gcgcacgagc acgcaacgct
      181 ccttcgggcc cagcgcgcgc tcgctcagca cctggatgcg atcgtggccg gcaatgccga
      241 agccgcgcat gcgcttcatg agccacgcga gtccggcgat gagcgcgacg atggcgagca
      301 gtgccagcgt cacctcgacg agacctccgc ccgaactggg cgcggtgctc acggcgggcg
      361 cggcgaacag gggtgcgtcc acggtttact tgagcttgcg gatgcgttcg gcggtgctga
      421 ccacgtcggt gatgcggatg ccgaatttct cgttgaccac cacgacttcg ccgtgtgcga
      481 cgagcgtgcc gttgacgaac acgtcgagcg gctcaccggc cgcgcggtcc agctccacga
      541 cgctgccctg gttgagctgc agcaggttgc ggatcgggat acgcgtgcgg cccacttcca
      601 tggacagggt gacgggaacg tcgaggatca cctcgagatt cacatcctgg gattcgccgt
      661 cggcaaccac cgcctcactc ttcgcattca tcatttcacc tcgagttgtt ttgattgcgc
      721 cggtatttcg tttcgtaccg gacgcattgc tggccgcgcg agacgccgta cgcgcccttg
      781 aacagcggca cgccttcggc aagcacggtc gccttgccgg tgaagtcgca gggcaatacg
      841 tcgcccggct tgagatccac gagcttcgac aaggcgacgc tgccgttgcc gagcacggtg
      901 gtcagttcga cttccgcatc ctggagtccg tccttgagca tcgtgagcca gcggtcgtcc
      961 tgctcggcgc gatcgctctg tacgcccgag tcgagcactt cgcgcagagg ctcgatcatc
     1021 gaatacggca tgacgacgtg cagcgaaccg ccgccgccgt cgagatcgat ctggaacggg
     1081 cacaccacca cgacttccga cggggtgacg atgccggcga aattggggtt gatctccgag
     1141 ttgacgtatt cgacgtcgat cttcgcgatc ggcgtccacg cttctttcat gtccgtgaac
     1201 gcgctgcgca ggaacatgtg cacgacgcgc atttcgaccg acgagaattc gcggccttcg
     1261 atcttcgcct ggcggccaac gccgccgaag aagttgtcga ccatcgcgaa cacgagtttt
     1321 ggatcgatca cgaacagcgc cgtgccgcgc agcggatgga ccttcacgag attcaggttg
     1381 accggcagcg acagcgagtg tgtgtactcg ctgaacttct tcatggacac ctgccccacc
     1441 gcgatctcga cctggcgtcg cagcaggttg tagatgctga cgcgaagctg gcgcgcgaga
     1501 cgctcgttga ccatctcgag cgtcggcatg cggccgcgca cgatgcgcat ctcgcggctg
     1561 aaatcgtatt ggcgtgcttc gcccggattc ggcgcgggat cggtgctgac cgcgccctgg
     1621 tcgacgccgt tgagcagcgc gtcgatttca tcctgattga gaacttcctg gccggccatg
     1681 agggcgccct actgcatcac gaatgaagtg aagtacaacg cctcgacctt gggtccgtcg
     1741 ccgcccgatt ccacgaccac cgtgcggacg gcttcgaggc aacgtgtacg cagcgcttcc
     1801 ttgccttcga ccttggagac gctttcgtaa ttctggccgc cgaggatcag cagaaggtcg
     1861 ttgcggatgc gcgggtcgtt gtccttgatg atcttttcga tctcgaggtc gcgcgtcatg
     1921 atgccgatgg tgatctgcag gaagcgcacc gacgattcgg cttcgaagtt cacgacgaac
     1981 ggcgggtcca gcgtgacgta acgcgccggg agcaattgct ctttcttggc gtcttccttc
     2041 ttcttcggat cttccttgcc gccgagtagt ttgaacgcgg cgaatccgcc accgccgagc
     2101 accagcagca acaccagtcc gatgatcagg cccttcttgc cgcccttcgc gggcgcggtt
     2161 tcggcgccct cttccagggt ctcgtcggtt gcggcgggtt gggccatgct gaatcctcac
     2221 gcggaaaagt agttgcgacg ggaattcatc gtgcaacgcg cgtgccacgt cccaagcgtg
     2281 atgggcgtca cgctaaggcg gcgtctggcg gcaatccgtg acggtttttt ggcgtaggag
     2341 gtgggtcgaa aaaaggggac agatctattt tccgcagcgg aaaatagatc t
//
LOCUS       DATDEU010000002         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1459361, whole genome shotgun sequence.
ACCESSION   DATDEU010000002 DATDEU010000000
VERSION     DATDEU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459361"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VIV63_00025"
     CDS             complement(<1..681)
                     /locus_tag="VIV63_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEY6123006.1"
                     /translation="MDTALQDRIRSTQQRHVIDDASKVGEARRAAHTLANFELTADIA
                     DKVAIAATELANNLLIHAGGGELLIQTLGNDEAMVFELLAIDRGRGMDNVAKCLTDGY
                     STAGTPGTGLGAVKRLATDFDIYSVPDEGTIVMARFGAGLPANYGAVSLPLEGEVDCG
                     DGWCLRTHDEGLSLFVVDGLGHGTFAAEAAHAAIGAFKDNPFAEPKDIMVRANGLMSK
                     TRGGSAACA"
     gene            complement(687..1094)
                     /locus_tag="VIV63_00030"
     CDS             complement(687..1094)
                     /locus_tag="VIV63_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010883997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEY6123007.1"
                     /translation="MTASVKSGEAPVRSEHDIVMARQQVRRLTQELKFSLVDQTKMVT
                     AASELARNALIYGLGGTMFWDVLDADGRTGLRLKFADEGPGIPNMELAMTDGWTSGKG
                     LGMGLTGTKRLVNDFEIDSAPGAGTRVTITRWK"
     gene            complement(1078..1461)
                     /locus_tag="VIV63_00035"
     CDS             complement(1078..1461)
                     /locus_tag="VIV63_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6123008.1"
                     /translation="MEKIPILRMGEFLLVTIQIDMHDQLALKLQDDLSNAINKYASRG
                     VLIDISSLEMVDSFIGRMVADISGIGRILGAETILVGMQPAVAITLVELGLSLPGVAT
                     ALNVERGMALLRRKIDADGADDSLR"
     gene            complement(1470..2297)
                     /locus_tag="VIV63_00040"
     CDS             complement(1470..2297)
                     /locus_tag="VIV63_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6123009.1"
                     /translation="MSNVLKKNGKEIHASWAKDLASTQSNSKGRISATELERQAAEFI
                     ELLTIASEHAKASDITAPEFKPLRDFLDGVSRSRVAQGFSSDETASFIFSLKKPFFAA
                     LRADAGKDADALADQVWRATELLDALGLHTVKAYQKSREDVINRQQREMLELSTPVVK
                     LWEGILALPMIGTLDSARTQVVMENLLQKVVETGAQIAILDITGVPTVDTLVAQHLLK
                     TVTALRLMGAECIISGVRPQIAQTIVHLGVDLQGVTTKANLADALALALRRTGQSLK"
BASE COUNT          430 a          787 c          815 g          513 t
ORIGIN      
        1 cgcacaggcg gccgacccgc cacgcgtctt cgacatgagg ccgttggcgc gcaccatgat
       61 gtctttgggt tcggcgaacg gattgtcttt gaacgctccg atggcggcat gggctgcttc
      121 ggcggcgaac gtgccgtgtc ccaggccatc gacgacgaac agcgaaagtc cctcgtcatg
      181 ggtcctgagg caccagccat cgccgcaatc cacttcgcct tcgagaggca ggcttacggc
      241 gccgtaattc gcgggcaagc cggcgccgaa ccgcgccatg actatcgtgc cttcgtcggg
      301 caccgaatat atgtcgaaat cggtggcgag gcgcttcacc gcgccgagtc ccgtgcccgg
      361 cgtgcccgcg gtcgagtaac cgtccgtcag gcatttcgcc acgttgtcca tgccgcggcc
      421 acgatcgatc gccagcagct cgaacaccat ggcttcgtcg ttgcccagcg tctggatcag
      481 gagttcgccg ccgcccgcgt gaatcagcag gttgttagcc agttccgttg ccgcgatggc
      541 gactttgtcg gcgatatcgg ccgtgagctc gaagttggcg agcgtatgcg ccgcacggcg
      601 cgcctcgccg accttgctcg cgtcgtcgat cacatggcgt tgttgcgtgc tgcggatgcg
      661 gtcttgcaaa gcggtgtcca tggaacctat ttccatcgcg taatcgtcac gcgcgttccc
      721 gcgccaggcg cgctgtcgat ctcgaaatcg ttcaccagcc gtttggtacc ggtgagaccc
      781 atgcccagcc ctttacccga ggtccagccg tcggtcatgg cgagctccat gttcggaatg
      841 cccggccctt cgtcggcgaa cttgagccga agtccggtgc gcccgtcggc gtccagtaca
      901 tcccagaaca tcgtgccgcc gaggccgtag atgagggcat tgcgcgcaag ctcgctggcc
      961 gccgtgacca tcttggtctg atcgacgagc gagaatttga gctcctgcgt gagtcgccgg
     1021 acctgctgcc gggccatcac gatgtcatgc tccgagcgca ccggcgcctc gcccgattta
     1081 acggaggctg tcatcggcgc cgtccgcgtc tatcttccga cgcaataacg ccatgccacg
     1141 ctcgacgttg agagcggtcg cgactccggg caatgacagc ccgagctcga ccagggtgat
     1201 cgccaccgcc ggttgcatgc ccaccaggat cgtctcggcg ccgaggattc tcccgatgcc
     1261 ggagatgtcc gcgaccatgc ggccgatgaa agagtcgacc atctcgagcg acgagatgtc
     1321 gatgagcacg cctctgctgg cgtacttgtt gatggcgttg gatagatcgt cctgcaactt
     1381 caacgcaagt tgatcgtgca tgtcgatctg gatggtgacg agcaggaact cgcccatcct
     1441 gaggattggg attttctcca tctgccgctt tatttcagcg actggccggt gcgcctgagc
     1501 gcaagggcca gcgcgtccgc gagattcgcc ttcgtcgtca ctccctgcag gtccacaccg
     1561 aggtgcacga tggtctgcgc gatctgcggg cgcacgccgg agatgatgca ttccgcgccc
     1621 atgaggcgca gcgccgtgac cgtcttgagc aagtgctgcg cgacgagtgt gtccaccgtg
     1681 gggacgcctg tgatgtcgag gatcgcgatc tgcgcgccgg tctccacgac cttctgcagc
     1741 agattctcca tgaccacctg ggtgcgagcg gagtcgagcg tgccgatcat cggcagcgcc
     1801 aggattcctt cccagagttt cacgacgggt gtcgacagtt cgagcatctc gcgctgctga
     1861 cggttgatga cgtcttcacg agacttctga tacgccttca ccgtgtgaag gcccagggcg
     1921 tccagtagtt cggtagcgcg ccagacctgg tcggcgaggg cgtccgcatc cttgcccgca
     1981 tccgcgcgca gcgcggcgaa gaacggcttc ttcagcgaga agatgaaact cgctgtttcg
     2041 tccgacgaaa atccctgagc gacgcgcgag cgcgatacgc cgtcgaggaa atcgcgtaac
     2101 ggtttgaatt cgggcgcggt tatgtcgctg gccttcgcgt gttcggatgc gatcgtcagc
     2161 aactcgatga actcggcggc ttgccgctcg agttcggtcg cggagatgcg gcccttgctg
     2221 ttcgactgtg tgctggcgag atctttcgcc caactggcgt ggatctcttt gccattcttc
     2281 tttaatacgt tcgacatcgt agcgtccgcc atcggacttc ctctgcgttg agtaaagtga
     2341 ggcgcgactg tagcaacggg gtgacgattg ttaggtaggc ggggtctgac tgctgtgaag
     2401 gacggcgccg tcaatagagt tggccgcgcg gaggcgaaga accgacaggg gagttgacaa
     2461 ggtccccggc gggagggccg ggatgtgccc tcgaggggtg cgaaaccggc caggagtgag
     2521 gtgaccggtg acgcccaagt atttg
//
LOCUS       DATDEU010000003         2263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1459441, whole genome shotgun sequence.
ACCESSION   DATDEU010000003 DATDEU010000000
VERSION     DATDEU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459441"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VIV63_00045"
     CDS             <1..466
                     /locus_tag="VIV63_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6123010.1"
                     /translation="VSEPLKPWLDPIIVSSSLHAYVSQSARGELVMGASLDPQELHST
                     RSTLDFAEGLAANMLDLFPFLSEVKVNRQWAGMADMTPDFAPIMGLTPCEGFFLDAGW
                     GTWGFKATPISGKTMAQTVATGTPHPLILGFSLERFARLELTGEKGAASVGH"
     gene            463..735
                     /locus_tag="VIV63_00050"
     CDS             463..735
                     /locus_tag="VIV63_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY6123011.1"
                     /translation="MKVIDCPLNGPRPLLEFVCAGEVRATPDPDTCSDAEWTAYLFNR
                     ASVPGVKREWWCHTPSGYWFVVERDTARDEILATYDAAQLAAKAGA"
     gene            732..>2263
                     /locus_tag="VIV63_00055"
     CDS             732..>2263
                     /locus_tag="VIV63_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6123012.1"
                     /translation="MMYRLPKVPGEWIDRSRTLEFRFEGRKFQGFAGDTLSAALAAAG
                     EMTLGRSFKYHRPRGIFSFANHDANNLFQVGEVPNVRGDVTPLLAGAEVYAVNTVGGV
                     RHDRARFLEWLAPFMPVGWYYKAFHGKNFRRWEHCIRKASGLGRIDADAPRVRTPKRY
                     AFCDVLVIGAGASGLATALSAADAGARVLLVDENAHAGGSAPWTGAVAGEVDSLVRRV
                     TGNPNIELLTATYAAGVYADRWVALVEPSRMAKVRAGAIVFATGAIEQPAVFRNNDLP
                     GVLLGSAAQRLLRRYAIAPGKRVTILTANREGYELARELRAHQVEVAAVLDLRAGFGG
                     DAHGLGDLRCVAGVTPEAAVAGRHGAVRALRVRVNEDESRPMLAETIACDAVIMSVGF
                     APASGLLSQAGALMHYDMALQQHVPDRLPPGIFAAGRVNGIYDYAARRQDGRDAGAEA
                     AAFARGGHVGSRVSRPDSVRQVSHPYPVFTHPAARDFVDLDEDIQVKDLLDSASQGFD
                     ST"
BASE COUNT          349 a          756 c          780 g          378 t
ORIGIN      
        1 ggtgtccgag ccgctgaaac cgtggctcga tcccatcatc gtgtcgagct cgctgcacgc
       61 ctacgtgtcg cagagtgctc gcggcgagct ggtcatgggc gcctcgctcg atccccagga
      121 gctgcactcg acgcgatcca cgctcgactt cgccgaaggg ctggccgcca acatgctcga
      181 tctgttcccg ttcctctcgg aagtgaaggt gaatcgccag tgggccggca tggccgacat
      241 gacgccggac ttcgctccga tcatgggtct cacgccctgc gaaggattct ttctcgacgc
      301 gggctggggc acctggggct tcaaggccac gccgatctcg ggcaagacga tggcgcagac
      361 cgtggcgacc ggcacgcctc atccgttgat cctcggcttc tcgctcgagc gcttcgcgcg
      421 cctcgagctc accggcgaaa aaggcgcggc atcggtgggc cattgaaagt catcgactgc
      481 ccgctcaatg ggccgcgccc gctgctcgag ttcgtctgcg caggcgaagt gcgcgccacg
      541 cccgatccgg acacctgcag cgacgccgag tggacggcct atctattcaa ccgcgcgagc
      601 gtgcccggcg tgaagcgcga gtggtggtgc cacacgccgt cgggttactg gttcgtcgtg
      661 gagcgcgaca cggcgcgcga tgaaatcctg gccacttacg acgccgcgca actggcggca
      721 aaggccggcg catgatgtac cgtctgccga aggtcccggg cgagtggatc gatcgttcgc
      781 ggacgctcga gttcaggttc gaaggccgga agttccaggg cttcgccggc gacaccctct
      841 cggcggcact ggcggcggca ggcgaaatga ccctgggacg cagtttcaaa taccaccgcc
      901 cacgcggcat cttctcgttc gcgaaccacg acgccaacaa cctcttccag gtcggcgagg
      961 ttcccaacgt gcgcggcgac gtcacgccgc ttctcgccgg cgcggaggtc tacgccgtga
     1021 acaccgtggg cggtgtcagg cacgatcgcg cgcgcttcct cgaatggctg gcgcccttca
     1081 tgccggtggg ctggtactac aaggcgtttc acggcaagaa cttcaggcgc tgggaacatt
     1141 gcatcagaaa ggcgtcgggg ctcggccgca tcgacgcgga cgcaccgcgc gtgcggacgc
     1201 ccaagcgtta tgccttctgc gacgtgctgg tcatcggtgc gggcgcgagc ggactggcaa
     1261 cggccttgtc ggctgcggat gcgggcgcgc gcgtcctgct ggtcgacgag aacgcgcacg
     1321 ccggcggcag cgccccgtgg accggcgccg tggccggtga agtcgactcg ctcgtcaggc
     1381 gcgtgactgg caatccgaac atcgaactgc tgaccgcgac gtacgcggcc ggcgtgtacg
     1441 ccgatcgctg ggtggcgctg gtggagcctt cgcgcatggc gaaggttcgc gcgggcgcca
     1501 ttgtgttcgc cacgggcgcc atcgagcagc cggccgtgtt ccgcaacaac gatctgccgg
     1561 gcgtgttgtt aggctcggcg gcgcagcgtc tcctgcgccg ctacgccatt gcgcccggca
     1621 aacgcgtgac gatattgacc gccaatcgcg aagggtacga actggcgcgc gagttgcgcg
     1681 cgcaccaggt cgaggtcgcc gccgtgctgg atctgcgcgc gggttttggc ggcgatgccc
     1741 acgggctggg cgacctgcgc tgcgtcgccg gcgtcacgcc cgaagcggcg gtggccggac
     1801 gccacggcgc cgtccgcgcg ctgcgtgtcc gcgtgaacga agatgaatca cgaccgatgc
     1861 ttgccgaaac catcgcctgc gatgcggtga tcatgagcgt gggcttcgcg cccgcgtccg
     1921 gactgctgtc gcaggcgggt gcgctcatgc attacgacat ggcgcttcaa caacatgtgc
     1981 ccgacagact gccgcccggc atcttcgcgg ccggccgggt caacggtatc tacgactatg
     2041 ccgcgcggcg ccaggacggg cgcgacgccg gtgcggaagc cgccgctttc gcgcgtggag
     2101 gccacgtggg atcgcgtgtt tcgcggcccg attccgttcg tcaggtgtcg catccctatc
     2161 cggtgttcac gcatccggcg gcgcgcgact tcgtcgatct ggacgaggac atccaggtga
     2221 aggacctgct cgacagcgcg agccagggct tcgacagcac cga
//
LOCUS       DATDEU010000004         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1021821, whole genome shotgun sequence.
ACCESSION   DATDEU010000004 DATDEU010000000
VERSION     DATDEU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021821"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VIV63_00060"
     CDS             <1..466
                     /locus_tag="VIV63_00060"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY6123013.1"
                     /translation="AAIEQRLRDVDRDLHQLAQQHPVAVRLQEIPGVGPLTATALVGS
                     VPHIHAFTRGRHFASWLGLTPRESSSGRRRTLGGISKRGDRYLRSLLAHGARSVLRAA
                     MVLARSPHRLTRLHQWGLQLRDRRGQNRAVIGVANKLARIIWAVWSRDLAFT"
     gene            complement(820..1263)
                     /locus_tag="VIV63_00065"
     CDS             complement(820..1263)
                     /locus_tag="VIV63_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123014.1"
                     /translation="MPLIGSPLHAQTPAALTSVQRLKCTFTQRALGTWDKDAVPSVEL
                     TPAKLSMVFDAIDTQDGTANVIDVTAPDAGAPHITVRLLANNLHLMAMNISGSVYLTS
                     VFADRDSSVGGTFKAVHTRHEFTPTRLTGWTSRPEQYYGQCQVEK"
     gene            1371..>2254
                     /locus_tag="VIV63_00070"
     CDS             1371..>2254
                     /locus_tag="VIV63_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123015.1"
                     /translation="MLSKFTTLTMFAATWVVVAVVSAGAQAQTARMRFESMDQNRDGA
                     ISRDEWRGSDRAFQNQDWNGDGQLSGNEVRLGARRDGNYEEADHLPNRYERYVSWTAA
                     GFTNLDHNRDRRISANEWHFDVETFRRVDRNRDGSLSQSEFMGGDIDDARDASFDDLD
                     WNNNGRVERSEWNGSDSVFTSLDRNRDGVLTRFEVVGGVNTPNDTYDEFASLDYNRNG
                     AISRDEWHWSAVSFDRRDTNRDGRLSKQEFAVSGGAPDAPGAVGTSGGAVQQRTVRVN
                     SQQRWTDSGIVVRAGDTLT"
BASE COUNT          412 a          690 c          728 g          424 t
ORIGIN      
        1 cgcggcgatc gaacagcgct tacgcgacgt ggaccgcgac ctccaccagc tcgcgcagca
       61 gcatccggtg gccgtgcggc tgcaggagat tccgggcgtg ggcccgctca cggccaccgc
      121 cttggtcggc tccgtgccgc atattcacgc gtttacccgg ggccgccatt ttgcgagttg
      181 gttggggtta acgccgcgcg agtcgtccag cggacgtcgt cgcaccctgg gcggcatcag
      241 taaacgcggg gatcgctacc tgcgcagtct gctggcccat ggtgcccgct cggtcctgcg
      301 tgccgcgatg gtgctcgcgc gatcgccgca ccgcctcacg cgcttgcacc agtgggggtt
      361 acagctgcgc gatcgacgtg gccagaatcg tgccgtcatc ggcgtggcga acaaactcgc
      421 gcggattatt tgggcggtgt ggagtcgaga cctggcgttc acctaaggcg gtcgacactc
      481 gtccgcggaa tgcgcagtga gaggatcctg tcatgaactg accggtcgga ccggcgccga
      541 cgcatgccga ggcaaaccgc tggctgtgag aggccttgtg aagatgattg gcacgcggcg
      601 cgcggatgct catgatggcc aggaggccgc acgaccgtct ccgcaacagg ccgaatatat
      661 gcgcgcatcc ggtttctgct gaccagacat gaaggacact tcttgctgac attcgggagg
      721 agtccatata tgcggtttcc cgcatcgtcg agctggttcg cggaagcaac cctggttcgc
      781 cctgccgtga ggctaagcaa tcagctgcgc agtaacggcc tatttctcga cctgacactg
      841 tccgtagtac tgctcgggcc gcgacgtcca gccggtcagc ctggtcggcg tgaattcgtg
      901 gcgagtgtgg acggctttga acgtgccgcc gacgctgctg tccctgtccg cgaacaccga
      961 cgtcagatag accgaccctg aaatgttcat cgccatcaga tgaaggttat tcgcgagcag
     1021 gcgcaccgtg atgtgcggcg cgccggcatc gggcgccgtg acgtcgatca catttgcggt
     1081 cccgtcctgc gtatcgatgg cgtcgaagac catcgacagc ttggccggtg tcagttcgac
     1141 gctggggacc gcgtctttgt cccacgtgcc gagcgctcgt tgggtgaacg tgcacttgag
     1201 gcgctggacg ctcgtcaacg cggcaggtgt ctgcgcgtga agcggcgatc cgatcagggg
     1261 catcaacact acggcaaagc gcaggcatcg catcattcga aaactctcct cgctcacagc
     1321 ccgttgttac atacggtacg gtctgtgcaa ctcctcaaac ggaggacgtt atgttgtcta
     1381 aatttactac gctcaccatg tttgcagcca cgtgggtggt tgttgcggtt gtttccgctg
     1441 gcgctcaggc gcagacggcg cggatgcgat tcgaatcgat ggaccagaat cgcgacggcg
     1501 ccattagccg cgacgagtgg cgcggctctg atcgcgcctt tcaaaatcag gactggaatg
     1561 gcgacggcca actctccggc aacgaggtcc gtcttggcgc gcgccgcgac gggaactacg
     1621 aggaggccga ccacctgccg aatcgatacg agcggtatgt cagctggacc gctgccggct
     1681 tcaccaacct cgatcacaac cgcgatcgtc gcatcagcgc aaacgaatgg catttcgatg
     1741 tcgagacgtt ccggcgcgtc gaccgcaatc gcgacggctc cctcagccag tccgaattca
     1801 tgggtggtga tatcgacgat gcccgcgatg cgagcttcga cgatctcgac tggaacaaca
     1861 acggccgcgt cgagcgttcc gaatggaatg gcagcgactc ggtattcacg agcctcgatc
     1921 gcaaccgcga cggcgtgctg acccgtttcg aggttgtcgg cggcgtgaac acaccgaatg
     1981 acacctatga tgagttcgcc tcactggact acaaccgcaa tggtgcgatc agccgcgacg
     2041 agtggcattg gtcggccgtg tcgttcgacc ggcgcgatac caaccgcgac ggcaggctct
     2101 cgaaacagga gttcgcggta agcggcggcg cgccagatgc tccaggcgca gtcggcacgt
     2161 cggggggtgc agtgcagcag cggacggtgc gtgttaactc tcaacagcgg tggaccgact
     2221 cgggcatcgt cgtacgtgcc ggagatacgc tgac
//
LOCUS       DATDEU010000005         3068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_146670, whole genome shotgun sequence.
ACCESSION   DATDEU010000005 DATDEU010000000
VERSION     DATDEU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146670"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VIV63_00075"
     CDS             complement(<1..717)
                     /locus_tag="VIV63_00075"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123016.1"
                     /translation="MNLRALAERRYAGSAIAVVAGVLLALSFAPFACWPLAILAPAIL
                     LWLWDGAAPRRAAVLGFWFNAGTFSVGTYWLYIAIKVIGHAPFLLAMFLMIGLVAIMG
                     AYHALLGWAVAKFLPARGAMRWMVGIPGAWLFIEWWRSWFLSGFGWLALGYAHTDNWL
                     GGLAPVVGQFGLGLLTLVLAGALVTLLLGDRRSRIVAGASFAAIWAVAFGLRGIEWTK
                     PYSRPISVAVVQGAIPQDEKW"
     gene            complement(714..1607)
                     /locus_tag="VIV63_00080"
     CDS             complement(714..1607)
                     /locus_tag="VIV63_00080"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY6123017.1"
                     /translation="MNAKNKEKDKERDVGASGRWLRKLSRKIGGEAQDRGEVVEYLRE
                     AGLRGVIEGDALAMLEGVLGVADIHVRDIMVPRAQMTVLNGTDDGETLLKTVVESGHS
                     RFPVMDEDREKIIGVLLAKDLLRLAVKDTTPFDIKEFMRPAVFVPESKRLNVLLREMR
                     RSRVHMAIVADEYGGIGGLVTIEDIIEQIVGDIDDEHDVDEDVNIRKDGDRQYVVRGQ
                     TPIDEFNEYFQTELSDDEFDTVAGLVMKQLGRLPRRGETLQLADCELKVTRFDRRRID
                     SLRLTAPRDIVPPEDRPLESE"
     gene            complement(1604..2062)
                     /gene="ybeY"
                     /locus_tag="VIV63_00085"
     CDS             complement(1604..2062)
                     /gene="ybeY"
                     /locus_tag="VIV63_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY6123018.1"
                     /translation="MKLELARGTRAWTPARSRMNDWAASALGQRGEGREIAVRVVAAR
                     ESRALNKLWRGKDKPTNVLSFPAPDQSRRGMPKDMALPLGDLVICAQVVRQEAIRDGK
                     ALQSHWAHLVVHGALHLVGYDHEAGRLQRLRMERREISVLKKFGIGNPYR"
     gene            complement(2077..>3068)
                     /locus_tag="VIV63_00090"
     CDS             complement(2077..>3068)
                     /locus_tag="VIV63_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY6123019.1"
                     /translation="ELMATPAPIEPVREFDLVPPDNTRLANLCGPLDENLRLIEDRMA
                     VSVKRRGSNFRISGQRAHAAEDVVKDLFEATRHEDVSLERVHLALRDSRDMPGALGVH
                     EPADDGGSVRVARGTIRARGRNQKDYLTNIRAKDLSFGIGPAGTGKTYLAVACAVEAL
                     QADKIRRIILVRPAVEAGERLGFLPGDLTQKVDPYLRPMYDALYEMLGFDRVAKFIER
                     NVIEVAPLAFMRGRSLNDSFIILDEAQNTTPEQMKMFLTRIGFGSRAVVTGDVTQTDL
                     PAGKMSGLRHVINILRNTEGVSFTFFEAADVVRHPLVQRIVKAYEAHTPPDQL"
BASE COUNT          528 a         1030 c          989 g          521 t
ORIGIN      
        1 ccatttctcg tcctgcggaa tcgcgccttg caccaccgcg acgctgatcg gccggctata
       61 cggcttggtc cactcgatac cgcgcagacc gaaagccacg gcccagatcg ccgcgaacga
      121 tgcgcctgcg acgatgcgcg agcgccggtc gcccagcaac agcgtgacca gtgcgccggc
      181 cagcaccagc gtgagcagcc ccaggccgaa ctgtccgacg acgggagcca ggccgcctaa
      241 ccagttgtcc gtgtgcgcat aacccagcgc cagccagccg aagccggaca ggaaccagct
      301 acgccaccac tcgatgaata gccacgcccc gggaatgccc accatccagc gcatggcgcc
      361 gcgcgccggc aggaatttcg cgacggccca gccgagcaac gcgtgatagg cgcccatgat
      421 ggcgacgagg ccaatcatga ggaacatcgc cagcaggaac ggcgcgtggc cgatcacctt
      481 gatcgcgatg tacagccagt aggtgccgac ggaaaacgtt ccggcgttga accagaaccc
      541 gagcaccgcg gcgcggcgcg gggcggcgcc gtcccacagc cacaggagga ttgccggcgc
      601 gaggatggcg agcggccaac acgcgaatgg cgcgaacgac agcgccagca acacgcccgc
      661 gacgaccgcg atggcgctgc cggcataacg gcgctcggcc aatgcccgca ggttcactcg
      721 ctttctaacg gccggtcttc cggcggcacg atgtcgcgcg gcgccgtgag ccgcagggaa
      781 tcgatgcgcc ggcgatcgaa gcgcgtgacc ttgagctcgc aatcggcgag ctgcagcgtt
      841 tcaccgcgac gcggcagacg gcctaactgc ttcatgacca ggcccgcgac cgtgtcgaat
      901 tcgtcgtcgg agagctcggt ctggaagtat tcgttgaact cgtcgatcgg cgtctgcccg
      961 cgcacgacgt actggcggtc gccatccttg cggatgttca cgtcctcgtc gacgtcgtgt
     1021 tcgtcgtcga tgtcgcccac gatctgctcg atgatgtcct cgatggtgac gaggccaccg
     1081 atgccgccgt attcgtcggc gacgatggcc atgtgcacgc gcgaacggcg catctcgcgc
     1141 agcagcacgt tgagccgctt ggactccggc acgaacaccg cgggacgcat gaattccttg
     1201 atgtcgaacg gcgtagtgtc ttttactgca agccgcagca ggtccttcgc gagcagcacg
     1261 ccgatgatct tctcgcgatc ctcgtccatc acggggaaac gcgaatggcc gctctcgacc
     1321 acggtcttga gcagcgtctc gccgtcgtcc gtgccattga gcacggtcat ctgcgcgcgc
     1381 ggcaccatga tgtcgcgcac atggatgtcg gcgacgccga gcacgccttc gagcatggcg
     1441 agcgcgtcgc cttcgatgac gccgcgcagc cccgcctcgc gaaggtattc gacgacttca
     1501 ccgcgatcct gcgcttcgcc gccgatcttg cgcgacaact tgcgcagcca tcggccgctg
     1561 gcgccgacgt cgcgttcctt gtccttttcc ttgttcttgg cgttcaccgg tagggattcc
     1621 cgatcccgaa tttcttgagc acggaaatct cccggcgctc catgcgcagg cgttgaaggc
     1681 gtccggcctc gtggtcgtaa ccgaccaggt gaagcgcgcc atggaccacc agatgcgccc
     1741 agtgcgactg cagggctttg ccatcgcgaa tcgcttcctg acgaactacc tgcgcgcaga
     1801 tcacgagatc gccgagcggc aaagccatgt ctttgggcat gccccgccgc gactggtccg
     1861 gcgctggaaa cgacagcacg ttggtgggtt tgtccttgcc gcgccagagc ttgttcaggg
     1921 cacgactctc gcgggctgcg acgacgcgaa ccgcgatctc gcggccctcc cctcgctgcc
     1981 cgagcgccga agccgcccag tcgttcatgc gcgagcgcgc cggcgtccag gcgcgggttc
     2041 cgcgagcgag ttcgagtttc aacgcccgct ctgaactcat agctggtcgg gaggcgtatg
     2101 cgcttcgtac gccttcacga tgcgttgtac gagcgggtgc cggaccacgt cggccgcttc
     2161 gaagaaggtg aacgagacac cttcggtgtt gcgcaggatg ttgatcacgt gccgcaggcc
     2221 cgacatcttg ccggccggca ggtccgtctg cgtgacgtcg cccgtcacca ccgcgcgcga
     2281 gccgaagccg atgcgcgtca ggaacatctt catctgttcg ggcgtcgtgt tctgcgcttc
     2341 gtcgaggatg atgaaggagt cgttgagcga ccggccacgc atgaaggcga gcggcgccac
     2401 ttcgatgacg ttgcgctcga tgaacttggc gacgcgatcg aagccgagca tttcgtagag
     2461 cgcgtcgtac atggggcgca ggtacggatc caccttctgc gtgagatcgc cgggcaggaa
     2521 ccccaggcgc tctccggcct cgacggccgg tcgcacgaga atgatccggc ggatcttgtc
     2581 cgcctgcagc gcttcgacgg cgcacgccac cgcgaggtaa gtcttgcccg taccggcagg
     2641 gccgatgccg aacgacagat ccttcgcgcg aatgttggtg aggtaatcct tctgattgcg
     2701 gccgcgcgcg cgaatggttc cgcgagcgac gcgcaccgag cctccgtcgt cggcgggttc
     2761 atggacgccg agcgcgcccg gcatgtcgcg cgaatcgcgc aacgccaggt gcacgcgctc
     2821 gagcgagaca tcttcgtgac gcgtcgcttc gaacagatct ttcaccacgt cttcggccgc
     2881 gtgcgcacgc tggccggaga tccggaagtt ggagccgcgg cgtttcaccg acacggccat
     2941 gcggtcttcg atgagacgca ggttttcgtc gagcgggccg cacaggttcg cgagccgcgt
     3001 gttgtccggc ggcacgagat cgaattcgcg caccggttcg atgggagcgg gagtggccat
     3061 caattcgc
//
LOCUS       DATDEU010000006         2112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1897287, whole genome shotgun sequence.
ACCESSION   DATDEU010000006 DATDEU010000000
VERSION     DATDEU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897287"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VIV63_00095"
     CDS             <1..623
                     /locus_tag="VIV63_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6123020.1"
                     /translation="VWQEEGRGRLSETPREVFDVHIEPGGHHIQVPPGENVLTAALAA
                     GIPLPYSCRAGRCASCKAKLLSGRIEYPLGTPPGITATEIAKGEVLLCQAQPRSDLSV
                     EARRVPVRVSNAASCEIVALEPLPLRALRLRLRFVEGRIDARPGQFVDARNHAGDAER
                     LAVIGGGAGILELECADDGSVFRAWLDASAVPGSGLRIAGPFDRPR"
     gene            complement(634..1365)
                     /locus_tag="VIV63_00100"
     CDS             complement(634..1365)
                     /locus_tag="VIV63_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY6123021.1"
                     /translation="MRVLIVDDEPPARERLRSMLAEIGEFEVAGEAGNGEQAIDLVDK
                     LIPDIVLLDVRMPGIDGLEVARHLAALPEPPSVIFTTAFDEYALQAFDSEAVAYLLKP
                     IRAEKLRAALTKAARLTRPQLQQVAAATREPAHRLHIGVRGRDGLKLIPIEEVFYFHA
                     DQKYTTVKHQKGEDLIEDSLKTLEEEFTATFVRIHRNALVNTRYLERIARDATGQHFV
                     HLRGLADALEVSRRMAGDLKDRFRI"
     gene            complement(1368..>2112)
                     /locus_tag="VIV63_00105"
     CDS             complement(1368..>2112)
                     /locus_tag="VIV63_00105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6123022.1"
                     /translation="YYFGSGELFPDDHWTFLARNAGITFVVTALALRYFYVTHEWRHN
                     VELQARARVHALQARIRPHFLFNSMNTIAALTRSNPARAEEAVQDLADLFRANLNEKN
                     NQISLAEEIDVARTYQRMEQLRLGDRLVVDWKIDSLPTDAMVPGLTLQPLLENAIYHG
                     IEPRPGGGVVTVTGEFNKGMITIVVKNPVPAANLTMRDGNRLALANIKERLDLMYGER
                     ATVKAGRFDDEYIVTLRFPFSSTPGTNIR"
BASE COUNT          346 a          637 c          735 g          394 t
ORIGIN      
        1 ctgtttggca agaagaagga cgaggccgcc tgagcgaaac gccgcgcgaa gttttcgacg
       61 ttcacatcga gccgggcggt caccacatcc aggtgccgcc cggcgagaac gtgctgactg
      121 ccgcactggc cgccggcatc ccgcttccct atagctgccg tgcggggcgc tgcgcttcct
      181 gcaaagcgaa gctgctgtcg gggcgtatcg aatatcccct gggcacaccg cccggtatca
      241 ccgcgaccga gatcgccaaa ggcgaagtgc tgctttgcca ggcacagccg cgcagcgatc
      301 tgtcggtgga agcgcgtcgt gtgccggtgc gggtttcaaa cgcggcgagc tgtgaaatcg
      361 tggcgctcga gccgctgcct ttgcgtgcgc ttcggctgag attgcgattc gtcgaaggcc
      421 ggatcgatgc gcgcccgggc cagttcgtcg acgcgcgcaa ccacgccggc gatgcggagc
      481 ggctggcggt catcggcggc ggtgcgggaa ttctcgagct ggagtgtgcg gacgatggca
      541 gcgtgttccg cgcgtggctg gacgcgagcg cggtgccggg gtcgggtttg cgcatcgccg
      601 gtccgttcga ccggccgcgc tgagcaacgc ggatcagatg cggaagcggt ccttgagatc
      661 gcccgccatg cgccggctga cttcgagtgc gtccgccaac ccgcgcagat gcacgaaatg
      721 ctggccagtg gcgtcgcgcg cgatgcgctc caggtatcgc gtgttcacga gcgcgttgcg
      781 gtgaatgcgc acgaaggtcg cggtgaattc ttcttcgagt gtcttcagcg aatcctcgat
      841 gaggtcctcg cctttctgat gcttgaccgt ggtgtatttc tggtcggcgt gaaagtagaa
      901 cacttcctcg atcgggatga gcttgaggcc atcgcggccg cgcacaccga tgtgcaggcg
      961 atgcgccggc tcgcgtgttg cggcggctac ctgctgcaat tgcggccggg tgagccgcgc
     1021 ggccttggtg agcgcggcac gcagcttttc cgcgcggatc ggcttgagca gataggcgac
     1081 ggcctcggag tcgaacgcct gcaacgcgta ttcatcgaac gcggtggtga agatcactga
     1141 cggcggctcg gggagcgccg ccaggtggcg cgccacttcg agaccgtcga tgcccggcat
     1201 gcggacgtcg agcaacacga tgtcgggaat gagcttgtcg accaggtcga tggcctgctc
     1261 gccattgccg gcctcaccgg cgacttcgaa ttcgccgatt tccgcgagca tcgagcgaag
     1321 gcgttcgcgc gccggcggct cgtcgtccac gatgagaacg cgcaggctca tcggatattg
     1381 gtgccgggcg tcgatgaaaa cgggaagcgc agcgtcacga tgtactcatc gtcgaaacgt
     1441 cccgccttca ccgtggcgcg ctcgccgtac atgagatcga gccgttcctt gatgttcgcg
     1501 agcgcgagcc ggttgccatc gcgcatcgtg agattggcgg cgggaaccgg gttcttgacc
     1561 acgatcgtga tcatgccctt gttgaattcg cccgtgaccg tgaccacgcc gccgcccggc
     1621 cgcggttcga tgccgtggta aatggcgttc tcgagcagcg gctgcaacgt gaggccgggc
     1681 accatcgcat ccgtgggcag ggaatctatt ttccagtcca cgaccagccg gtcgccgagc
     1741 cggagctgtt ccatgcgctg gtaggtacgg gcgacgtcga tctcctcggc gagcgagatc
     1801 tggttgttct tttcattgag gttggcgcga aacaagtcgg caagatcctg cacggcttcc
     1861 tcggcacgcg ccggattgct gcgcgtgagc gccgcgatgg tgttcatgct gttgaagagg
     1921 aaatgcggac ggatgcgcgc ctgaagcgca tgcacgcgcg ctctcgcctg cagctcgacg
     1981 ttgtggcgcc actcgtgcgt gacgtaaaaa tagcgcagcg cgagcgcggt gacgacgaag
     2041 gtgatgcccg cgttgcgcgc gaggaaggtc cagtgatcgt cggggaacaa ttcgccgctg
     2101 ccgaagtagt ag
//
LOCUS       DATDEU010000007         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1313844, whole genome shotgun sequence.
ACCESSION   DATDEU010000007 DATDEU010000000
VERSION     DATDEU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313844"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VIV63_00110"
     CDS             complement(<1..286)
                     /locus_tag="VIV63_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123023.1"
                     /translation="MEMFDASGRINEARHSSASGFTLAIPLLFIITGTAVAQTPPPPP
                     PAESEAEAEGLEAIVVTARRRAERLQDTPVAVTALSAEALERQQIVSTTDL"
     gene            399..884
                     /locus_tag="VIV63_00115"
     CDS             399..884
                     /locus_tag="VIV63_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY6123024.1"
                     /translation="MPRTSNLKRRDLPDEFEAPELMFSELDGLLGYQLRRAQGAMHRD
                     FMAAVADFGLTQKQAAALWLIQANGGVSQVEVAAALGMDRATMMAVTDRLEDRGFVTR
                     KRSLTDRRRQELYLTPSGLSTLRKCKSRIAEHEEKFRAMFTAAELAALISALKKFQTP
                     V"
     gene            887..>2186
                     /locus_tag="VIV63_00120"
     CDS             887..>2186
                     /locus_tag="VIV63_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY6123025.1"
                     /translation="MSVKRVRVERDGELALIVAQNPPVNTITAEVRAGLREALEEIRS
                     ASDIRAVVLMCEGSTFFSGADIGEFSGPPREVEYRTLFNGFEALEFPVIAAMHGTVMG
                     GGLEIALACHYRVASPTTRFALPEVTLGIIPGAGGTQRLPRLIGAERALEFIVSAKPV
                     SAQQAQELGFLDQTIEGELKSSAVAYARGLLAGGKGPRRTGEMKVDPATATDQVFEKS
                     AQQARKLYPNRDAAVVAVEAVRKASQSSLAEGLEFETSLVNDRKTSVESRGAIHVFFA
                     ERDSRRVPGLSDDVRASPVKSAAVIGAGTMGGGIAICFANAGIPVTLLDTSDAALQRG
                     LANIDGVYQSMVERGRLTAADKTQRQALIRTTLDYGALRDADVIVEAVFESMDLKREV
                     FGKLEAVARPGAILATNTSTLDIEVIANATSRPEDVIGMHF"
BASE COUNT          412 a          682 c          706 g          386 t
ORIGIN      
        1 cgagatcggt ggtgctgacg atctgctgcc gctctagcgc ttcggcgctg agggccgtca
       61 ccgccacggg cgtgtcctgc aggcgttcgg cgcggcgccg cgcggtgacg acgatggcct
      121 cgaggccttc ggcttccgcc tcgctttcag caggcggtgg tggtggcgga gtctgcgcga
      181 ccgccgtacc agtgatgatg aaaagtagcg gaatcgcaag cgtgaagccg cttgcggacg
      241 agtgccgcgc ttcgttgatg cggcctgaag cgtcaaacat ttccatggag cccccccggg
      301 ttgaaatatt gtacgtaaca catactattt gtattgcgag cggcttggca agcggggtag
      361 attcgcgcca gctctttaac accactctta gaggcccgat gccgcgcacc tcaaacctga
      421 aacgtcgtga cctgccggac gagttcgaag ccccggaatt gatgttctcg gagctcgatg
      481 ggctgctggg ctaccaacta cgtcgtgcgc agggcgccat gcaccgggac ttcatggccg
      541 cggtggccga tttcgggttg acgcagaagc aggccgccgc gctctggctc atccaggcga
      601 acggcggcgt ctcccaggtc gaggtggccg cggcgctcgg aatggatcgc gccaccatga
      661 tggcggtgac ggaccggctc gaagatcgcg gcttcgtgac gcgcaagcgg tcgctgaccg
      721 accggcgccg ccaggagctg tacctcactc cgtcggggct gagcaccttg cgcaagtgca
      781 aatcgcgtat cgccgagcac gaagagaaat tccgcgcgat gttcacggcc gctgaactcg
      841 cggcgctgat cagcgccctc aagaaattcc agactccggt ttgatgatgt ccgtgaagcg
      901 tgtgcgcgtc gaacgcgacg gtgagctggc gttgatcgtc gctcagaatc ctccggtcaa
      961 taccatcacg gccgaagtgc gcgccggact tcgcgaagcg ctcgaggaaa tccggtctgc
     1021 cagtgacatc cgcgccgtcg tgctgatgtg cgagggcagc acgttcttct cgggcgcgga
     1081 tatcggcgag ttcagtggtc cgccgcgcga ggtcgaatac cgcactttgt tcaacggttt
     1141 cgaagcactc gaatttccgg tcatcgccgc catgcatggc accgtcatgg gaggcggact
     1201 cgagattgcg cttgcctgtc actatcgcgt cgcttcgccg accacgcgtt tcgcgctgcc
     1261 cgaagtgacg ctcggcatca ttccgggagc gggcggcacg caacgattgc cccggctcat
     1321 cggcgccgaa cgggcgctgg agttcatcgt gtcggccaag ccggtcagcg cacaacaagc
     1381 gcaggaactg ggcttcctcg accagaccat cgaaggcgaa ttgaaaagca gcgcggtcgc
     1441 ttacgcgcgc ggattgcttg ccggcggcaa gggtccgcgc cgaaccggcg agatgaaagt
     1501 cgatccggcg accgcgaccg accaggtgtt cgaaaaatcc gcgcaacagg cgcgaaagct
     1561 ctaccccaac cgggatgcgg ccgtggttgc cgtggaggcg gtgcgcaaag cctcgcaatc
     1621 cagcctcgcg gaaggactgg aattcgaaac ctcactcgtc aacgaccgca agaccagcgt
     1681 cgagtcccga ggcgcgatac acgtgttttt cgccgaacgc gacagccgtc gtgtgcccgg
     1741 cttgagcgac gacgttcgtg cgtcgccggt caagtcggcc gcggtcattg gcgccggcac
     1801 catgggcggc ggcatcgcca tctgctttgc gaacgccgga ataccggtga cgctgctcga
     1861 caccagcgat gcagcactgc aacgaggact cgccaacatc gatggcgtct accaatccat
     1921 ggtggaacgt ggccgactca cggcagcgga caagacccag cggcaggcat tgatacgaac
     1981 gacgctcgac tatggcgcat tgcgcgatgc cgacgtcatc gtcgaagccg tattcgaaag
     2041 catggacctg aagcgtgaag tgttcgggaa gttagaggcg gtcgcgcgac ccggagccat
     2101 tctggccacc aacacctcca cgctggacat cgaggtcatc gccaatgcca cctcgcgtcc
     2161 cgaggatgtg atcggcatgc acttct
//
LOCUS       DATDEU010000008         3313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1101, whole genome shotgun sequence.
ACCESSION   DATDEU010000008 DATDEU010000000
VERSION     DATDEU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VIV63_00125"
     CDS             complement(<1..1138)
                     /locus_tag="VIV63_00125"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY6123026.1"
                     /translation="MNIRLDRPVDPQRDHALGPARAEITLVEYGSYDCPHCRAANARI
                     GEVREQLGDRVRYVFRHRPISGSELARRAAELVEQASTPDEFWSAHVSLMTRSRTLTE
                     DDLRAVADDLRLGENSTEAAASLEARARERVDADVASSHASGVRFTPTFFINSRRYDG
                     PWDEASFTDAMLGSLGHRVRTAALGFASWAPSTGLLLLLATLLAVVLTNSAAGPAFTR
                     FWETPLSLGFGDAAFSMSLVHWVNDALLTVFFLVVGLEIKREFTVGHLASRRSAALPI
                     AAAVGGMVVPALIYVSVVPSGPWAFGWGVPMATDTAFAVALIAVMGSRVPVELRIFLT
                     AAAIVDDIGAIIVVAIFYSDALNLTALAGAVAATGFLVLLNRSGV"
     gene            1191..3221
                     /locus_tag="VIV63_00130"
     CDS             1191..3221
                     /locus_tag="VIV63_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6123027.1"
                     /translation="MDLEHGLRPYRNLAYAVVLFAVCSAAIFMAPANETPFVHTFFDA
                     GIVVVSGVLALLLWDMGSRVADRLTRLLAVAIGVTAAFELIHVLPALEVSRDAVDAAR
                     IAGLVRPVTWPPAAFLLPIGLGAAYLLRKRNTNVLPAFGVGLSITGGLLLWIFDPIPP
                     YTAPTWFGITRPSLVLVPILSIFVGVLYWRVRRTERLARIIGLLTLLALVSSIAMLYS
                     EAPADRSSMTAHAARFANDLFLLVTLSQLGSIETARRMRAEYDLTLANEALEDRVRAR
                     TADLEAAYSELRATQQAAMQQERLRALGQMASGIAHDINNAISPASLYVESILARAPG
                     LDDRTRNQLETVQRAIGDVAQTVSRMGEFYRQGESRATLAPVNVNAILSQMPDLTRAR
                     WSDLAQARGAVIDMRVEEAAGVPTIMANESELREALINLVFNAADALPDGGTITLRAR
                     REAADGPLALPTIVVEVSDDGVGMDEDTRHRCLEPFFTTKGERGTGLGLAMVYGIAQR
                     HCAELQIDSEKGSGTTIRLMFPDSEGATAVVKNREARSGAPALRLLLIDDDPLILQSL
                     RNALELEGHNVTCASGGQMGIDLFRAAFERSEPYSAVITDLGMPHVDGRHVAAAIKAI
                     ARTARVIMLTGWGHLLADGSEVPAHVDQILSKPPDMWELREALAQAVQAAEA"
BASE COUNT          588 a         1084 c         1104 g          537 t
ORIGIN      
        1 acaccccgga gcgattgagc aacaccagga aacccgtggc cgccaccgca ccggcgagcg
       61 cagtcaggtt gagtgcatcg gagtagaaga tggccacgac gatgatggcc ccgatgtcgt
      121 caacgatggc cgccgcggtc agaaagatgc gcaactccac cggcacgcgc gagcccatga
      181 cggcgatcag cgccaccgcg aacgcggtat ccgtggccat gggcacgccc cagccgaaag
      241 cccagggccc gctggggacg accgacacat atataagagc gggaacgacc atgccgccca
      301 ccgcggctgc gatgggcagg gcggcgctgc gccgcgaagc gaggtgccca acggtgaatt
      361 cgcgttttat ctcgagaccc acgacgagga agaacacggt cagcaatgca tcgttcaccc
      421 aatgcacgag cgacatgctg aacgccgcgt cgccgaagcc gagcgacagt ggcgtctccc
      481 agaatcgcgt gaacgcaggg cctgccgccg agttggtgag gacgacggcc aacagcgtgg
      541 ccagcaacag cagcaagccg gtggagggcg cccagctcgc gaatcccaac gccgcggttc
      601 gcacgcggtg gccgagtgaa cccagcatcg cgtcggtgaa cgacgcctca tcccacgggc
      661 cgtcgtagcg gcggctgttg atgaagaacg tgggcgtgaa gcgcacgccg ctcgcatgcg
      721 aactggccac atccgcatcc acgcgttcac gcgcgcgcgc ttcgagactc gcggcggctt
      781 ccgtcgaatt ctcgccgagc cgcagatcgt cggccacggc gcgcagatcg tcttcggtca
      841 gcgtgcgcga acgggtcatc agcgaaacat gcgcgctcca gaattcgtcg ggcgtcgagg
      901 cctgctccac cagttccgcg gcacgacggg cgagctcgct acccgagatc gggcggtggc
      961 ggaatacgta tcgaacacga tcgccgagct gctcgcgcac ttcgccgatg cgcgcattgg
     1021 cggcgcgaca gtgggggcaa tcgtagctgc cgtattcgac gagagtgatc tcggcgcggg
     1081 ccggtccaag agcatggtca cgttgcggat cgacggggcg atcgaggcga atgttcatgg
     1141 cgcaattaga acacgtagaa tcgcgtggct cttccggggg aggcgttcgc atggacctcg
     1201 agcatggatt gcggccctac cgcaatctcg cttatgccgt cgtgctcttt gccgtttgct
     1261 cggcggccat cttcatggcc cccgcgaacg aaaccccttt cgttcatacg tttttcgacg
     1321 ccggcatcgt cgtggtgagc ggcgtgctgg cgctgctgct gtgggacatg ggctcgcgcg
     1381 tcgccgacag gctcacgcgc cttctggccg tggccatcgg cgtcacggcg gctttcgaac
     1441 tcatccacgt gcttcccgcg ctcgaggttt cgcgagacgc ggtggatgcc gcccggatcg
     1501 ccggtctggt gcgtccggtg acgtggccgc cggccgcgtt cctgctgccg atcggtctcg
     1561 gcgcggcgta cctgctgcgc aaacgaaaca cgaacgttct tcccgcgttc ggcgtcggcc
     1621 tgtcgatcac cggcggtttg ctgctctgga tcttcgatcc cattccccca tacacggccc
     1681 cgacctggtt cggcatcacc cggccttcac tggtgctggt gccgatcctg tcgatcttcg
     1741 tcggtgttct ctactggcgc gtgcgccgca ccgaacgcct cgcacgcatc atcggattgc
     1801 tgacgttgct cgcgctcgtc agcagcatcg ccatgctcta ttcggaagcg cccgcggacc
     1861 ggtcgtcgat gacggcgcac gccgcccggt tcgcgaacga tctgttcctg ctcgtcacgc
     1921 tctcgcagct cgggtcgatc gagactgcgc gcaggatgcg cgcggagtac gacctcacgc
     1981 tggccaacga ggcgctcgaa gaccgggttc gcgcgcgaac cgccgatctc gaagcggcgt
     2041 actccgagct gcgcgccacg caacaggcgg ccatgcagca ggagcggctg cgcgcgctcg
     2101 gccagatggc gagcggcatc gcgcacgaca tcaacaacgc gatctctccg gcatcgctgt
     2161 acgtcgagtc catcctggcg cgcgcgccgg ggctcgacga tagaacgcgc aatcagctcg
     2221 agaccgtcca gcgcgccatc ggcgacgtcg cgcagaccgt gtcgcgaatg ggcgagttct
     2281 atcggcaggg cgaatcgaga gccacgctcg cgccggtgaa cgtcaacgcc atcctgagcc
     2341 agatgcccga tctcacgcgg gcgcgctgga gcgacctggc gcaggcacgg ggcgcggtca
     2401 tcgacatgcg cgtcgaggaa gcggccggtg tgcccacgat catggccaac gagagcgagc
     2461 ttcgcgaagc gctcatcaac ctggtgttca acgccgccga cgcattgccc gacggtggga
     2521 cgatcacgct gcgtgcgcgg cgcgaggccg ccgatggacc actcgcgttg ccgacgatcg
     2581 tcgtcgaagt cagcgacgat ggcgtcggca tggacgaaga cacgcggcac cgttgcctgg
     2641 agccgttctt cacgaccaag ggagagcgcg gcacgggact cggccttgcc atggtgtatg
     2701 gaatcgcgca gcgtcattgc gcggaactgc agatagatag tgagaagggc agcggcacga
     2761 cgatccgcct gatgttcccg gattcggaag gcgcgaccgc tgtcgtgaag aaccgcgagg
     2821 cgcggtctgg agcgccagca ctaaggcttc tcctgatcga cgatgacccg ctcatcctgc
     2881 agtcgctgcg caacgcgctc gaactcgaag ggcataacgt cacctgcgcg agcggcggac
     2941 agatgggcat cgacctgttt cgcgcggcct tcgaacgcag cgagccttac tccgcggtca
     3001 taaccgacct cggcatgccg catgtagatg ggcgtcacgt tgcggcggcg atcaaggcga
     3061 ttgcgcgtac agcgagggtt atcatgctga cgggttgggg acacctgctc gcggatggca
     3121 gcgaagtgcc ggcccacgtc gaccagattc tcagtaaacc tccggacatg tgggaactgc
     3181 gcgaagcgct cgcgcaggcc gtgcaggctg cggaggcata ggaaggcaat gcacgatccg
     3241 cgatcgctga atcccggtcg cgagatgtcg ctggtggccg cgctgctgat catggcgatg
     3301 atcgcggtgg tca
//
LOCUS       DATDEU010000009         4555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1460024, whole genome shotgun sequence.
ACCESSION   DATDEU010000009 DATDEU010000000
VERSION     DATDEU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460024"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..2059
                     /locus_tag="VIV63_00135"
     CDS             146..2059
                     /locus_tag="VIV63_00135"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123028.1"
                     /translation="MTDVGITWRDKLRYRFENTLSKGTVAIIGWLALLSIAIVMLAAL
                     ILAIMRVGQDPADHEHPYGFFEGMWQSMLRTLDPGVVAGDNGWQLRLLMMIVTIGGIF
                     IVSILIGTITSGLEARLAELRKGRSRVIETDFTLILGWSSKVYSIIGELLIANSNQKN
                     PCIVILAERDKIEMEDDIRAKFPSTANTRVICRSGNPLDLDDLAVVDPHSARSIIVLA
                     PETENPDIHVIKSVLAITNNPARKSEPYHIVAEIREPRNLEAAALVGGKETIYVQGED
                     LIARVTAQTCRQSGLSVVYTELLDFDGAEIYFKAEPGLAGRTYREVISAYEDSTVMGI
                     MRANGETLLNAPMDTTFASGDLVIAITEDDDTLVLSATSGGGLPDVGALSHRERLPAA
                     PERILILGWNGKGQAIIEELDNYVAQGSETIVICRQEGVRDILMGVAKRMKRQKVRFA
                     DGDITHSATLAAVKAATFEHVILLSYNELPIQEADAKTLITLLHLRNLADAAGVKLSI
                     VSEMMDLRNRALAQIARADDFIVSDKLVSLMMSQLSENRKLEQVFKVLFSSEGSEIYI
                     RPMTDYIKLGTTVDFYTVLEAAAQRGETAIGYRLMKYSDDKDHGYGVNVNPKKSEKHT
                     FTERDQIVVLAEG"
     gene            2059..2937
                     /gene="bla"
                     /locus_tag="VIV63_00140"
     CDS             2059..2937
                     /gene="bla"
                     /locus_tag="VIV63_00140"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEY6123029.1"
                     /translation="MIARRELLAGAIVLGLTSGIGAAESADKALKRIRERVGGRLGVH
                     ALDSQSGKRWGIDENSRYAMASTFKLPLAAALLWQVDRKAFTLDHLLTIAPKEILPNS
                     PAVEAKLAAGADAMSIRELCVAVITQSDNAAANTLLHGIGGPVALNAFFRTLGDETTR
                     LDRREPALNTNIPGDPRDTTTPRAMVDTMLRIFTQDVLSIPSRALLIDWMIASRTGLD
                     RVRAGLPKTWNPGDKTGTGENGAFNDLVIAYPPGRRPVFVAVYMSDSKLGAKELATAH
                     VEIGTLIAKVFKETKK"
     gene            2934..3437
                     /locus_tag="VIV63_00145"
     CDS             2934..3437
                     /locus_tag="VIV63_00145"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6123030.1"
                     /translation="MRALLAIFLVLVTCPAALAQKAGPAQTRQQYEEILAADQALFSA
                     FFDRCDVAALATMVTDDFEMFHDKNGFMAKSGKEFIDAIAGTCARQKTGEDYRARREL
                     VPGTLKVYPLNNYGAIEVGVHRFYQLLPGKPEKLVEVSQLTHVWKKEESGWKLARVLS
                     YDHQLTE"
     gene            complement(3452..3949)
                     /locus_tag="VIV63_00150"
     CDS             complement(3452..3949)
                     /locus_tag="VIV63_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY6123031.1"
                     /translation="MDVYLVRHAIAHERNATRWPNDALRPLTTLGMRKFRKAAAGLAR
                     CVPKSAAVFSSPFVRARETAAILTEHGKFRAATESVELVPGKSPRAVFDLLRSRRRNA
                     VVLIGHEPDLSAWLSTALAVEPLRIKSEFKKGGAACIRFEKTIGPGGATLVWLLPPRV
                     LRAMR"
     gene            complement(3949..>4555)
                     /locus_tag="VIV63_00155"
     CDS             complement(3949..>4555)
                     /locus_tag="VIV63_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY6123032.1"
                     /translation="TQRRNQARARLLRHLDSDRTRAWVAHWTEQLRLGTASGARAREA
                     VTATVALDLIRSAAGKLRKKANRLNDESSADDFHRVRIRAKRLRYVLDAFGGLYGDAA
                     REYLDALAKLQHVLGDYHDSTVRAGLFAELVTRGRNVPAATSFLVGRLVERDQSDFKK
                     CRRKFLKAYRRIKRRRWRALLAEMREQARAAGSPPDGAGVT"
BASE COUNT          865 a         1469 c         1437 g          784 t
ORIGIN      
        1 cgcacaggat gtccccttcg agcgcaagat tgccgacgtc ctgcgccagc tcgaacggcg
       61 ggaggcggtt atcgtttacg accccggcag cgacagcgtg gatatcgtgg tgcggcggcc
      121 gggtggttag ctaatatccg acgccatgac tgacgtcggg atcacgtggc gcgacaaact
      181 gcgctaccgg ttcgagaaca ccctttccaa gggcaccgtc gccatcatcg gctggctggc
      241 gctgctgtcc atcgcgatcg tgatgctggc cgcgctgatc ctggcgatca tgcgcgtcgg
      301 gcaggacccg gcggaccacg agcatcccta cggcttcttc gagggcatgt ggcaaagcat
      361 gctgcgtacg ctcgacccgg gcgtggtggc cggcgacaac ggctggcagc tccggctgct
      421 catgatgatc gtgaccatcg gcggcatctt catcgtcagc atcctcatcg gcaccatcac
      481 ctcgggtctc gaggctcgtc tggcggagct gagaaaaggc cgctcgcgcg tcatcgagac
      541 cgacttcacg ctcatcctcg ggtggtccag caaggtctac tccatcatcg gcgaactgct
      601 gatcgcgaac tcgaaccaga aaaatccgtg catcgtgatc ctcgccgagc gcgacaagat
      661 cgaaatggaa gatgacattc gcgcgaagtt tccgagcacc gcgaataccc gggtgatctg
      721 ccgcagcggc aatccgctcg atctcgacga cctggccgtg gtcgatccgc actccgcgcg
      781 ttcgatcatc gtgctcgccc ccgagaccga aaaccccgac atccacgtta taaagtccgt
      841 gctggcgatc acgaacaacc ctgcgcgcaa gtccgagccg taccacatcg tggcggagat
      901 tcgcgaaccc aggaatctcg aggccgcggc gctggtcggc ggcaaggaaa ccatctacgt
      961 gcagggcgaa gacctgatcg cgcgcgtgac cgcgcagacg tgccggcagt ccggcttgtc
     1021 ggtcgtctac accgagctgc tcgatttcga tggcgcggag atctacttca aggcggaacc
     1081 cggcctcgcg ggccgcacct accgagaagt gatttccgcg tacgaggact ccacggtcat
     1141 gggcatcatg cgcgccaacg gcgaaacgct gctcaatgcg ccgatggaca ccacgttcgc
     1201 cagcggcgac ctggtgatcg cgatcaccga agacgatgac accctggtgc tgtcggcgac
     1261 ttccggcggc ggcctgcccg atgtcggcgc gctgtcgcat cgcgaacgct tgcccgccgc
     1321 gccggagcga atcctgatcc tcggctggaa cggcaagggc caggcgatca tcgaagaact
     1381 cgacaactac gtcgcgcaag ggtccgagac catcgtgatc tgccggcagg aaggtgtgcg
     1441 cgacatcctg atgggggtgg ccaagcgcat gaagcgccag aaggtccggt tcgccgacgg
     1501 cgacattact cactcggcca cgctcgccgc ggtgaaggcc gcgactttcg agcacgtgat
     1561 cctgttgagc tacaacgagc tgcccatcca ggaagccgac gccaagacgt tgatcacgct
     1621 gctgcattta cgcaacctgg ccgatgccgc cggtgtgaag ctctccatcg tcagcgagat
     1681 gatggatctg cggaatcgcg cgctggcgca gattgcgcgc gccgacgact tcatcgtgag
     1741 cgacaagctc gtcagcctga tgatgtcgca gctgtcggag aaccggaagc tcgaacaggt
     1801 cttcaaggtg ctgttcagct cggaaggttc ggagatctac atccggccca tgaccgacta
     1861 catcaagctc ggaaccactg tggatttcta cacggtgctc gaagcagccg cgcaacgcgg
     1921 tgaaaccgcg ataggctacc ggctcatgaa atattccgac gacaaggacc acggctacgg
     1981 ggtcaatgtg aatccgaaga aatccgagaa acacacgttc accgagcgcg accagatcgt
     2041 cgtgctggcg gagggctgat gatcgcgcgg cgcgagttgc ttgcgggcgc catcgttctc
     2101 gggctgacgt caggtattgg cgcggccgag agcgccgaca aagccttgaa gcgaattcgc
     2161 gagcgcgtcg gtgggcggct cggggttcac gcgctcgact cgcagagcgg caagcgctgg
     2221 ggcatcgacg agaattcgcg atacgcgatg gcgtccacat tcaaactacc gctcgccgcg
     2281 gcgctgttgt ggcaggtgga tcgcaaggcg ttcacgctcg atcacctgtt gaccatcgcg
     2341 ccgaaggaaa ttcttcccaa ttctccggcg gtggaggcga agctggccgc gggtgcggat
     2401 gcgatgagca ttcgcgagtt atgcgtcgcg gtgatcaccc agagtgacaa cgccgccgcc
     2461 aatacgctgc tccacggcat tggcggtccc gttgcgctca acgcgttctt tcgcacgctc
     2521 ggcgacgaga ccacccggct cgaccgccgg gagccagcgc tcaacaccaa catcccgggt
     2581 gatccgcgcg acaccaccac gccgcgcgcc atggtcgaca cgatgctccg catattcacg
     2641 caggacgtgt tgtccattcc ttcgcgagcg ttgctcatcg actggatgat cgcgtcgcga
     2701 acgggcctcg accgcgtgcg cgcgggtctg cccaaaacct ggaatcccgg cgacaagacc
     2761 ggcaccggcg aaaacggcgc cttcaacgat ctcgtgatcg cgtatccacc cggccgtcgt
     2821 ccggttttcg tggccgtcta catgagcgac tcgaagctcg gcgccaagga actcgccacg
     2881 gcgcacgtcg agatcggtac cctgatcgcc aaggtcttca aggagacaaa gaagtgagag
     2941 ccctgctggc gatattcctc gtgctggtca catgccccgc cgcgcttgcg caaaaggccg
     3001 gccccgcgca gacccggcag cagtacgagg agatcctcgc cgccgaccag gcattgttct
     3061 ccgcgttctt cgatcgctgc gacgtggcgg cgctcgccac catggtcacc gacgacttcg
     3121 agatgttcca cgacaagaac ggattcatgg cgaagtcggg gaaggaattc atcgacgcga
     3181 tcgccggcac gtgcgcgcgg cagaagaccg gtgaggacta tcgcgcgcgc cgcgagcttg
     3241 tgccaggcac cctcaaggtc tatccactca acaactacgg cgccatcgaa gtcggcgttc
     3301 atcgtttcta ccagctgctg cccggcaagc cggaaaagct cgtggaagtg tcacagctca
     3361 cgcatgtctg gaagaaggaa gagagcggct ggaagctcgc gcgcgtgctg agctacgacc
     3421 atcagctgac ggaataaacg tcgccgacgc gttaccgcat cgcgcgcaac acgcgcggcg
     3481 gcaacagcca gaccagcgtc gcgccaccgg gaccaatggt cttctcgaag cgtatgcacg
     3541 ccgccccgcc tttcttgaat tcactcttga tgcgcaaggg ttcgacggct agcgccgtcg
     3601 agagccaggc gctcaggtcc ggttcgtggc cgatgagcac gacggcattg cgtcgccggg
     3661 agcgcagcag gtcgaaaacc gcacgcggcg atttgccggg gactagttcg accgactcgg
     3721 tcgcggccct gaacttccca tgttcggtca ggatcgcggc ggtctcgcgc gcgcgcacga
     3781 aggggctgga gaacaccgcc gcggacttcg gcacacatcg cgccagtccc gccgcagcct
     3841 tgcgaaattt ccgcatcccg agcgtggtca gtgggcgcag cgcgtcgttc ggccaccgcg
     3901 tggcattgcg ctcatgcgca atggcgtgtc tgacgagata aacgtccatc acgtgactcc
     3961 agcgccatcc ggcggacttc cggccgcgcg tgcctgttcg cgcatttccg cgagcagcgc
     4021 gcgccagcgg cggcgtttga ttcgccggta cgccttcagg aacttgcgcc ggcatttctt
     4081 gaaatcgctt tgatcacgtt cgacgagccg ccccacgaga aaggatgtcg ccgccggcac
     4141 gttccgtcct cgcgtgacca attccgcgaa cagacccgcg cgcaccgtgg agtcgtgata
     4201 atcgccgagg acatgttgca gttttgcgag cgcgtccaga tattcgcgtg ccgcatcgcc
     4261 gtaaagaccg ccgaaggcgt cgagtacgta ccgcaggcgc ttggcgcgga tgcgcacgcg
     4321 gtggaagtcg tcggcactcg attcatcgtt gagccggttg gctttcttgc gcagtttgcc
     4381 ggccgccgag cggatgagat cgagtgcgac agtcgcggta acggcctccc gcgcacgggc
     4441 gccgctcgcg gtacccaggc gtagctgctc cgtccaatgg gctacccagg cgcgggtgcg
     4501 gtcggaatcg aggtggcgta gcaagcgagc gcgtgcctgg ttacgccgtt gcgtc
//
LOCUS       DATDEU010000010         2491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_147402, whole genome shotgun sequence.
ACCESSION   DATDEU010000010 DATDEU010000000
VERSION     DATDEU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147402"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VIV63_00160"
     CDS             complement(<1..1153)
                     /locus_tag="VIV63_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6123033.1"
                     /translation="MYQQSAAAQSATSASATSTAGVEEIDEVIVTGVRASLEKSLEVK
                     RNANSRLEVVTAEDVGKLPAHNVADALRGLPGVNISSSSADEGGFDESDRVSLRGTSP
                     SLTQTLINGHSVGSADWFVLSQGNNVGRSVSYTLLPSELVNSVEVNKSSHARLQEGGT
                     TGTVNIITRKPFQFDKSLTVEASLGTVFSDQADSYDPQLSGLINYTNDSETFGVLLQV
                     FKQERSLRRDAQEIPGGFFTIGATDPAGIANPALVGVQAPGLIGSTLFEMVRERTGGL
                     LELQFKPSDNLALGLSGFVSDMDANNYNRNFMLWGGNFARAQAPNAGYTVENGVLTSA
                     TYAGVAGRDYAVYDMISREATAKSNYITFDADWQITDTFKSRLQLGTTEG"
     gene            1421..2152
                     /locus_tag="VIV63_00165"
     CDS             1421..2152
                     /locus_tag="VIV63_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY6123034.1"
                     /translation="MQLLAPLDESSDQPLYQQLQRALRAAIENRVIGPDDALPPERDL
                     ADMLSVSRITVRKAIDELVEEGLLVRKQGSGTFVSNRVEKNFAKLTSFSEDMRARGRE
                     PRSVWINKAAGTVTPEESLTLRSSPGTPVFRFHRIRYADDAPMALEYATVLRDCLPSL
                     EAVENSLYEALEQKGNRPVRALQRLRAVLLSAEQAKLLKAQERDAGLLVARVGYLKDG
                     RAVEFSQSYYRGEIYDFVAELSASS"
     gene            2149..>2491
                     /locus_tag="VIV63_00170"
     CDS             2149..>2491
                     /locus_tag="VIV63_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123035.1"
                     /translation="MSATTRMFHEASEAPQVVGAQLTHNAALLRDLGARLRNQPPRAV
                     VTCARGSSDHAATYAKYLIESHTHVLTSSAAPSVSSIYEAQADLDGVLFIAISQSGMS
                     PDLLTATRDARE"
BASE COUNT          472 a          762 c          775 g          482 t
ORIGIN      
        1 caccttcggt ggttcccaac tggagcctgg acttgaatgt gtcggtgatc tgccagtcgg
       61 catcgaaagt gatgtagttg ctcttcgccg tcgcttcgcg cgagatcatg tcgtagaccg
      121 cgtagtcgcg gccggccacg cctgcgtacg ttgcactggt cagcacgccg ttctcgaccg
      181 tgtatcccgc gttgggcgcc tgggcgcgcg caaagttgcc gccccagagc atgaagttgc
      241 ggttgtagtt gttagcgtcc atgtccgaaa cgaagccgct caatcccagg gccaggttgt
      301 cggaaggctt gaactgcagt tcgagcaggc cgccggtgcg ttcgcgaacc atttcgaaca
      361 gcgtcgaacc gatgaggccc ggcgcctgca ctccaaccag agccgggttg gcgatgccgg
      421 ccggatcggt ggcgccgatc gtgaagaaac cgcccggaat ttcctgcgca tcgcggcgca
      481 gtgaacgctc ttgcttgaag acctgcagca acacaccgaa ggtttcgctg tcgttcgtat
      541 agttgatcag tcccgacagc tgcggatcgt atgaatccgc ttggtcggaa aacacggtgc
      601 cgagcgaagc ctcgacggtc agcgacttgt cgaattgaaa cggcttgcgc gtgatgatgt
      661 tgacggtgcc ggtggtgccg ccctcctgca gcctggcgtg tgaagacttg ttgacctcca
      721 ccgagttgac gagctcggac ggcagcaatg tgtagctcac gctgcggcca acgttgttgc
      781 cctggctcag cacgaaccag tccgccgaac cgacgctgtg gccgttgatc agcgtctggg
      841 tcaggctcgg gctggtgcca cgaagactga cgcgatcgga ttcgtcgaag ccgccttcat
      901 cggcgctgga cgaactgatg ttcacgccgg gcagccctcg cagagcgtcg gccacgttgt
      961 gtgccgggag tttgccgacg tcttcggccg tcacgacttc caggcgcgag ttggcattgc
     1021 gcttcacttc cagcgatttc tcgaggctcg cgcggacgcc cgtgacgatg acttcgtcga
     1081 tttcctcgac gccggcagtc gaagtagccg aggcagaggt cgccgactgg gccgctgcgg
     1141 actgctgata caaggcgaaa gcaatcgccg tcccgatcaa ggtcgaccgt ctgatattca
     1201 tagagactcc taacccctgt tgttttgtgc ggaccaattg gaggccaata tagtaccaat
     1261 cgcataacga aaactagtct cttcaacacc aattcttcga atttgtcttt cacaggccta
     1321 agtgtggtga agaagaagca gattggtatt gcattggtta tatacagatg gcatggttca
     1381 cggggaaatt tgcagggggt aaggacactt ggcttcactg atgcagttat tggcgccgct
     1441 cgatgagtcg agcgatcaac cgctttatca acaacttcaa cgagcgctcc gcgccgccat
     1501 cgaaaaccgg gtcatagggc ccgatgacgc attgccgccc gaacgggatc tcgccgacat
     1561 gctttcggtc tcgcgcatca cggtgcgcaa agccatcgat gagctggtcg aagaaggcct
     1621 gctggtgcgc aaacagggct cggggacttt tgtcagcaac cgcgtcgaga agaacttcgc
     1681 gaagctcaca tcgttctccg aagacatgcg cgcacgcgga cgtgagccac gcagcgtgtg
     1741 gatcaacaag gccgccggca ccgtgactcc cgaggagtcg ctgacgctgc gctcgagtcc
     1801 cggcacgccg gtattccgct ttcaccgcat tcgctacgcc gacgacgcgc cgatggcgct
     1861 cgaatacgcc acggtgctgc gcgactgcct gccgtcgctc gaagcggtgg aaaactcgct
     1921 gtacgaagcg ctggagcaga aaggcaatcg cccggtgcgc gcgctgcagc ggctgcgcgc
     1981 ggtgttgctc agcgccgaac aagccaagct gctcaaggcg caggagcgcg acgcggggtt
     2041 gttggtggca cgcgtcggat acctcaagga cggacgcgcg gtggaattct cgcagtctta
     2101 ctaccgcgga gagatctacg acttcgtggc ggagctcagc gcatcttcgt gagtgccacg
     2161 acgcgcatgt ttcatgaggc gtccgaagcg ccgcaagtgg tcggcgcgca gctcacgcac
     2221 aacgcggcgc tgctgcgcga cctcggagcc cggctgcgca accagccgcc gcgcgcggtg
     2281 gtcacctgcg cacgcggcag ttcggatcac gcggccacct acgcgaaata tctcatcgag
     2341 tcgcacacgc acgtgctcac ctcctcggcc gcgccttcgg tgagctcgat ctacgaagcg
     2401 caggcggacc tcgacggcgt gctgttcatc gccatttcgc agtccggcat gagccccgac
     2461 ctgctcaccg ccactcgcga tgcgcgcgaa g
//
LOCUS       DATDEU010000011         2436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2044087, whole genome shotgun sequence.
ACCESSION   DATDEU010000011 DATDEU010000000
VERSION     DATDEU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044087"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VIV63_00175"
     CDS             <1..710
                     /locus_tag="VIV63_00175"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEY6123036.1"
                     /translation="PPPSTLGRGAAFLAFIATGALLALFGQTYQTGADIYELFLTWAL
                     LGLPLVIAAQWSVATAAWVVVLNVALLLFCGWNPAGGLLWAVLGGMDFRPAHLVLGAA
                     WLNTALWFAFELWRVDAVPDWVRRLILSCAFGFGTWSGVLAVIDDGDPVAALGLAAAM
                     IAVAAYAMRRRTDVYPLAVVMGAFIIVSLVWLGTKMTFRDEGMFLLMALWLIGTSTAA
                     GRILTVLTRRWRAEVAA"
     gene            707..1723
                     /locus_tag="VIV63_00180"
     CDS             707..1723
                     /locus_tag="VIV63_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HEY6123037.1"
                     /translation="MSAAAPLDELMRARGVIAVDAPLAPAQVSDRPWFVALLQGAAGW
                     LAGIFLLVFLGLVFKPDSGGALFAIGAVLLVAAWFLYHADRDAVFLDQLALAISIAGQ
                     FGVAVSVVKDHPSGLNIALTALAVQIFVLAVMPNNTARTLAALFATIAWVFTVRYLFQ
                     PGSGDEIFFGEDRPDRSMSFGALTPLLCWALTWLPLLSLAGWLVLREPQWMASTLRRF
                     ARPALTGVLLGLSFGGIAAEPFSVFILGTGTLGMPFSWWALFPLLSIGLALASAYGAF
                     RIRSAGLLGFAVFAALLHLSRFYYLYGTTLLWKSVIMLAAGALLLMAGVLLRKRVANA
                     GAAA"
     gene            1720..2184
                     /locus_tag="VIV63_00185"
     CDS             1720..2184
                     /locus_tag="VIV63_00185"
                     /inference="COORDINATES: protein motif:HMM:NF025703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HEY6123038.1"
                     /translation="MNKLHTRNLLIAAVLLVLGAVNASIILKERIKTEGQRIFLALAP
                     VDPRSLMQGDYMALRFEIVNNIQSQVSGSALLFVDERGVATLNPSPATGGLRIRYRVR
                     NGQIWLGTNAYFFEEGAAQRFDGARFGEFRVDRESGEAVLVALADENLKPLR"
     gene            complement(2176..>2436)
                     /locus_tag="VIV63_00190"
     CDS             complement(2176..>2436)
                     /locus_tag="VIV63_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY6123039.1"
                     /translation="ENLAHAFEFLLAENPDSALAAAVAIQSAADNLAAHPLVGRRVEG
                     DIRELVISFGATGYIALYRFVVQQDQVRVLALRHQREIGYLP"
BASE COUNT          321 a          775 c          813 g          527 t
ORIGIN      
        1 ggccgccacc ttcgactctc ggccgcggcg cggcattcct ggccttcatc gccacgggcg
       61 cgctgctcgc gctcttcgga cagacctatc aaaccggcgc ggacatctac gagttgttcc
      121 tgacgtgggc attgttagga ctgccgctgg tgattgccgc gcagtggagc gtagccacgg
      181 ccgcgtgggt ggtggtcctc aatgtcgcgt tgctgctctt ctgcggctgg aaccccgccg
      241 gtggcttgtt gtgggctgtt ctcggcggca tggattttcg gccggcgcat ctcgttctcg
      301 gtgccgcgtg gctcaacacc gcgttgtggt tcgcgttcga actatggcga gtcgatgccg
      361 tgccggattg ggtgcggcgc ctcatccttt cgtgcgcatt cggcttcggc acctggagcg
      421 gcgtgctcgc ggtgatcgac gacggcgatc ccgtggccgc cctgggcctt gccgcggcca
      481 tgatcgcggt cgcggcctac gccatgcgcc gccgcaccga cgtctatcca ctcgcagtgg
      541 tgatgggcgc gttcatcatc gtgagcctcg tgtggctcgg tacgaagatg acgttccgcg
      601 acgagggcat gttcctgctg atggcgttgt ggctcatcgg cacgtcgacc gccgccggcc
      661 ggatcctcac cgtgctcacg cgtcgctggc gcgcggaggt ggcggcatga gtgcggccgc
      721 accactcgac gagctgatgc gcgcgcgggg tgtgatcgcc gtggatgcgc cgcttgcgcc
      781 tgcgcaggtt tccgaccggc cctggttcgt cgcgttgttg caaggcgctg ccgggtggct
      841 ggcgggaatc ttcctgctgg tgttcctggg cctcgtgttc aagcccgact ccggtggcgc
      901 gttgtttgcg atcggcgcgg tgctcctcgt ggcggcctgg ttcctgtacc acgccgatcg
      961 cgatgccgtc tttctcgatc agctcgcgct ggcgatctcc atcgcagggc agttcggcgt
     1021 cgccgtatcc gtggtcaagg accatccctc gggcctgaac attgcgctga ccgcgctcgc
     1081 cgttcagatc ttcgtgctgg cagtcatgcc taacaacacc gcgcgaacgc tggcggcgct
     1141 gttcgcgacc atcgcctggg tgttcacggt tcgatacctg tttcagccgg gaagtggcga
     1201 cgagatcttt ttcggcgaag atcgtcccga tcgttcaatg tctttcggcg cgctgacgcc
     1261 gctgctttgc tgggcgctga cgtggctgcc gctgctctcg ctcgcgggct ggctggtcct
     1321 gcgcgagccg caatggatgg cgagcactct tcgccgtttt gcgcgtccgg cgctgaccgg
     1381 cgtgttgctg ggcttgtcat tcggcggcat cgccgccgag cccttcagcg tattcattct
     1441 gggtaccgga acgctcggaa tgcccttctc gtggtgggcg ctgttcccgc tgctgtcgat
     1501 cggccttgcc ctggcatcgg cgtatggcgc cttccggatt cgcagcgccg ggttgttagg
     1561 cttcgctgtg ttcgccgcgc tgctgcatct gtcgcgtttc tattacctct acggcaccac
     1621 gctcctgtgg aagtccgtca tcatgctcgc ggccggcgcg ttgctgctaa tggcaggcgt
     1681 gctcttgcgc aagcgcgtgg cgaatgccgg agccgcggca tgaacaagtt gcatacccgc
     1741 aatctgctga tcgccgccgt attgctggtg ctgggcgccg tgaatgcgtc catcatcctc
     1801 aaggagcgca tcaagacgga aggccagcgc attttcctcg cgttggcgcc cgtcgatccc
     1861 cgttcgctca tgcagggcga ttacatggcg ctgcgattcg agatcgtcaa caacatccag
     1921 tcgcaggtgt ctggcagcgc gttgttgttc gtcgatgagc ggggcgtcgc gacgctgaat
     1981 ccgagcccgg ccacgggcgg actgcgcatc cgttaccgcg tgcgcaacgg ccagatctgg
     2041 ctcggcacca acgcgtattt cttcgaagag ggcgccgcgc agcgtttcga cggcgcgcgt
     2101 ttcggtgagt tccgcgtgga tcgcgagagc ggcgaggccg tgctggtggc gctcgccgat
     2161 gagaacctga agccgctacg gtagatagcc gatttcgcgc tgatggcgca gcgccagcac
     2221 acgcacctgg tcctgctgca ctacgaagcg atacaacgcg atgtatcccg ttgcgccgaa
     2281 ggagatgacc aactcacgga tatcaccttc gacgcgtcgg ccgacgagcg ggtgcgccgc
     2341 aagattatcg gcggcgctct gaatggcaac ggcagccgcc aatgcgctgt caggattctc
     2401 ggctaacagg aactcgaagg cgtgcgcgag gttctc
//
LOCUS       DATDEU010000012         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1314780, whole genome shotgun sequence.
ACCESSION   DATDEU010000012 DATDEU010000000
VERSION     DATDEU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314780"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /gene="ydiK"
                     /locus_tag="VIV63_00195"
     CDS             <1..1085
                     /gene="ydiK"
                     /locus_tag="VIV63_00195"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEY6123040.1"
                     /translation="RVMNVGPSQDLTRTTLGVLIIGVLLVASLWTMQAFIGPLVWATA
                     IVVATWPLLLWVQRHVGNSRALATTVMTIVMLVIFVVPFWAALGAMLDASADGVDVVR
                     SYLKTGLGPPPAWLSKIPFAGERITNEWLAISAGGPDQFAEAIRPYVKSVAAWMLAIT
                     GGFGGLLGHFLLTVIISAILYAQGETAAQGVRAFAMRLAGQRGDAAVILAGQSVQSVA
                     LGVVVTALVQTALVGLGFFVAGIPHPGLLTAIVFVLCIAQVGPILILIPAVIWMYSHA
                     SGVWATAFLIYSIPMALLDNFLRPILISRGVDLPLILIIAGVIGGLIAFGVIGLFIGP
                     VILAVTYSSLGVWIADRPVPETRVNP"
     gene            1082..1750
                     /locus_tag="VIV63_00200"
     CDS             1082..1750
                     /locus_tag="VIV63_00200"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HEY6123041.1"
                     /translation="MKVDCRAAARVVSLGAVVLLAGCVVNAPPVSERTPDGLVRVQST
                     QMDTVYVAPGATLGAYRSLILDPVHLAFKLDWQQRNPGVTAAQVELIRSQGSRIFYEV
                     FSSALTQQHGYPLTTQPGSNVLKVSASLTELDVSVAPGTSGSQSMFIVSPDDLTLTLE
                     LRDSQSGALLARAVDKEKGRAYGNLKVEGSVENSAEARRALEMWAGLLRKGLDTARGT
                     PPAP"
     gene            1777..2328
                     /locus_tag="VIV63_00205"
     CDS             1777..2328
                     /locus_tag="VIV63_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123042.1"
                     /translation="MKRSIVFAIIGILASIGCAAKEPFMKKDSVEVSATVEAIDTENR
                     LLSLRGPAGLATIVCGPDIVNFPQIRVGDEVKVTYSVAMAAGITKSKATPKTTLDTQA
                     QTAPVGSKPSASVGATIETTVQIESVDTSFDAVTFKRPDGFVRTIAVESPEGKKFIRT
                     LKKGDMVDVRYTEALAIAVVPAK"
BASE COUNT          414 a          766 c          782 g          496 t
ORIGIN      
        1 ggcgcgtaat gaatgttgga ccatcacagg acctgacgcg gaccacgctg ggcgtgctca
       61 tcatcggcgt tctgctggtg gccagcctgt ggaccatgca ggcattcatc gggccgctgg
      121 tgtgggccac ggccatcgtc gtcgccacct ggccgctgtt gctctgggtg caacgtcacg
      181 tcggcaatag tcgcgcattg gcgacgaccg tcatgaccat cgtcatgctg gtgatcttcg
      241 tcgtcccgtt ctgggcggcg ctcggtgcca tgctcgacgc gtcggccgat ggtgtcgacg
      301 tcgtgcgttc gtatctcaag accggattgg gcccgccacc ggcgtggctt tcgaaaatac
      361 cgtttgcagg cgagcgcatc accaacgaat ggctggcgat ttcggccggt gggccggacc
      421 agttcgccga agcgatccgc ccgtacgtca aatccgttgc cgcgtggatg ctggcgatca
      481 ccggaggctt cggcggtctg ctgggacact tcctgctcac ggtgatcatc tccgccatcc
      541 tctacgcgca gggcgaaacc gccgcgcaag gtgtgcgtgc tttcgcaatg cgtctcgccg
      601 gtcagcgcgg cgacgcggcc gtgatcctcg ccggacaatc ggtacagagt gtggcgctcg
      661 gtgtcgtcgt aacggcgctc gtgcagaccg cgctggtggg gctcgggttc ttcgtcgcgg
      721 gcattccgca tcccgggctg ctcaccgcca tcgtgttcgt gctctgcatc gcgcaggtcg
      781 ggcccatcct cattttgatt ccggcggtca tctggatgta ttcgcacgcc agcggtgtgt
      841 gggcgacggc gttcctcatc tattccattc cgatggcgtt gctcgacaac ttcctgcgtc
      901 cgatcctgat cagccgcggc gtcgacctgc cgttgatcct catcatcgcc ggcgtcattg
      961 gcggactgat cgcgttcggt gtcatcggcc tgttcatcgg tccggtgatt ctcgccgtga
     1021 cgtattcgtc actcggcgtc tggatcgcgg accgtcccgt accggaaacc agggtgaacc
     1081 catgaaagtt gattgtcggg ccgcggctcg ggtcgtgagc ctgggcgccg ttgtgctgct
     1141 cgccggctgc gtcgtcaacg cgccgcccgt gtcggagcgc actcccgatg gtctcgtgcg
     1201 ggttcaatcg acgcagatgg acaccgtcta cgtggcgccc ggagcaacgc ttggcgcata
     1261 tcgcagcctg atactcgatc ctgtgcatct ggcgttcaag ctcgattggc agcagcggaa
     1321 tcccggagtg actgctgcgc aggtcgagct catccgttcg cagggctcgc gaatcttcta
     1381 tgaagttttc tcgagcgcgc tgacccagca gcacggttat ccgctcacga cgcagccggg
     1441 atccaatgtt ctgaaggtgt cggcatcgct caccgagctc gatgtttccg ttgcaccggg
     1501 aacgtcggga agccagagca tgttcatcgt ttcgccggac gacctcacgt tgacgctgga
     1561 acttcgcgat tcgcaaagcg gcgcattgct ggcgcgtgcc gtcgacaaag agaagggccg
     1621 cgcgtacggc aatctgaagg tcgaaggctc ggtggaaaac tccgcggaag cgaggcgggc
     1681 gctcgagatg tgggctggcc tgttgcgcaa agggctcgat accgcccgcg gcaccccgcc
     1741 agcgccataa ccatctactg cagaaggaac ctgaaaatga aacgttcgat tgtctttgcg
     1801 atcatcggaa tcctcgcctc gatcggctgc gcggcgaagg aaccgttcat gaagaaagat
     1861 tcggtggagg tctccgcgac cgtcgaagcg atcgacaccg aaaaccgcct gttgtccctg
     1921 cgcggacccg cagggcttgc aaccatcgtg tgcgggccgg acatcgtcaa cttcccgcag
     1981 attcgcgtgg gcgacgaggt gaaggtgacc tacagcgtgg ccatggccgc ggggatcacc
     2041 aagagcaagg ccacgccgaa aacgacactc gacacgcagg ctcaaaccgc gccagtcggc
     2101 tcgaaacctt cagcgtcggt cggcgcgacg atcgagacga ctgttcagat agagtcggtg
     2161 gacacgtcgt tcgacgccgt gactttcaag cggccggacg gtttcgtgcg caccatcgcc
     2221 gtcgaatcgc ccgaaggaaa gaagttcatc cggacgttga agaaaggcga catggtggac
     2281 gtccgttaca ccgaggcgct ggcgatcgcc gtcgtccccg ccaagtagcc ttgctacctg
     2341 gcgagaacgt attcaggcgc gggggacttc agaccccgcg tttggcgcgt tccgccttgc
     2401 cgcctttgta agagcctttc gcgccggcgg ccttgccgac gaacttcttc ttgaagcc
//
LOCUS       DATDEU010000013         5131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1607247, whole genome shotgun sequence.
ACCESSION   DATDEU010000013 DATDEU010000000
VERSION     DATDEU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607247"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VIV63_00210"
     CDS             complement(<1..649)
                     /locus_tag="VIV63_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEY6123043.1"
                     /translation="MTVTAVLALEDGTIFNGQSIGAKGNTTGEVVFNTAMTGYQEILS
                     DPSYSRQIVTLTYPHIGNTGTTPEDLESPTAYAAGLVVRDVPPLASNWRKNEDLREFL
                     TRHKVVAISGIDTRKLTRILREKGAQAGCIMTGEKMDPAAAVRLAKKFPGLKGMDLAK
                     VVTTKRTYQWNEGSLWQPENRPVMRAHQRLHVVAFDYGVKRNILRMLSDRGCRMTV"
     gene            complement(899..1708)
                     /gene="dapB"
                     /locus_tag="VIV63_00215"
     CDS             complement(899..1708)
                     /gene="dapB"
                     /locus_tag="VIV63_00215"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY6123044.1"
                     /translation="MTTAALIGATGRMGTAIVRASVAGKTLAVVAAVASAGSKSTGLD
                     VGEVAGVKALGVSVLNELPPDLAKAQVAIDFSRPDLSLRALNVCRAARVPIVIGTTGH
                     GAEFDARIAAASRDIAVLVAPNTSIGVAVAQELVRIAARALPAEFDIEISEAHHKHKL
                     DAPSGTALALAESAARARGLDPHEDTLSGRAGQGARRDGEIGIASIRAGDIVGTHTVL
                     FAGLGERLTVGHEATDRAIFANGAIRAAVWLATQKPGRYTMANVLGLKTTG"
     gene            1790..2782
                     /locus_tag="VIV63_00220"
     CDS             1790..2782
                     /locus_tag="VIV63_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6123045.1"
                     /translation="MIRIGILGAARIAPRGIVTPANALLGAEVVGIAARTLERAQDFA
                     AQHSIPMAFGSYAELIARDDIDLVYVALPPSAHLEWCTAALDSGKHVLVEKPFASNAQ
                     DAAQMVAAARAANRHLIEGFHYRFHPLFERTLSALRNGDIGKIRHIEAVFNANLPDTP
                     GELRYIEALGGGALMDLGCYCMHWIRTVAGDEPTVVSARARCATPGVDLDIEADLAFT
                     SGPTATLKCSMQPDDGVLSRYLRVHGDKGVLELDNPVSPHSGSTLSIESPDSPMPHLV
                     AGGDTTFHFQLRHVIEVIEGREQPLTGGDDAIGNMRAIDAIYRAAGLRPRGLNA"
     gene            2793..4310
                     /gene="glpK"
                     /locus_tag="VIV63_00225"
     CDS             2793..4310
                     /gene="glpK"
                     /locus_tag="VIV63_00225"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY6123046.1"
                     /translation="MGAAPGFSSKFVLALDAGTTSSRSLLFDATGKVVALAQREFTQH
                     FPRPGWVEHDATEIWETQRATMAEVLEAARATPRDIVAVGITNQRETTVLWDRATGAP
                     LAPAIVWQDRRTAPACEQLRAAGCEPEIAARTGLLLDPYFSATKLAWLLDQIPGARAR
                     AERGDLAFGTVDSWLLFKLTGHRRHVTDATNASRTLLFNIHTGDWDDRLLELLRVPRA
                     CLPELVDSCLDAPAAIEIDLDGVSLPVTGIAGDQQAALFGQACFVPGMAKNTYGTGCF
                     ALMNTGAAPQASRHRLLTTVAWRRGATTQYAIEGGVFMGGATVQWLRDGLGMITSSDE
                     VEALAASVPDTGDVYLVPAFAGLGAPQWDAAARGAIVGLTRGTTRAHIARAALESIAF
                     QTADLIEAMQQDAGRPLSELRVDGGAARNDLLLQFQADLLGVPVLRPVNTETTASGAA
                     ALAGLGAGLWQSQQELAALWKLEKRFEPRMTRDEAGRRRARWSQAVERSRDWANA"
     gene            4307..5038
                     /locus_tag="VIV63_00230"
     CDS             4307..5038
                     /locus_tag="VIV63_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY6123047.1"
                     /translation="MTSRKHARSALLAIFAVTTLTVGWVLYQREGEPRYLNASRAEFV
                     GLFASPPSPDSPQTRAELDVLLAIQRNRTAKDVDAARADRKTEISQFAGALGLTPAQV
                     RSLPALDALAEQVEDDIRPYVRTAKHRFTRLRPYEVDSRLEPCIGNVRDDLSYPSGHA
                     TFGFVVAYLLADMVPERRRELIDRATEFARQRAVCGVHFPSDIEAGNKAAVWLAQHFL
                     ASEAYRKAVVPARNELRAAMRLPAR"
BASE COUNT          862 a         1744 c         1628 g          897 t
ORIGIN      
        1 ccaccgtcat gcggcaaccg cggtcggaga gcatgcgcag gatgtttcgt ttcacgccgt
       61 agtcgaaagc cacgacgtgc aggcgctgat gcgcacgcat caccgggcgg ttctccggct
      121 gccagagact gccttcgttc cactggtagg ttcgcttggt ggtgaccacc ttggcgaggt
      181 ccatgccttt caggcccgga aatttcttgg cgagacggac cgcggcggcc ggatccatct
      241 tctcgccggt catgatgcaa ccggcctggg cgcccttctc gcgcaggatg cgcgtcagct
      301 tgcgcgtgtc gatgccggag atggccacga ccttgtggcg cgtgaggaac tcgcgcaggt
      361 cttcgttctt gcgccagttg ctggcgagcg gcggcacgtc cctaactacc agccccgcgg
      421 cgtaggccgt gggcgactcg aggtcttcgg gcgtggtgcc tgtgttgcct atgtgtggat
      481 aggtcagcgt cacgatctgt cgtgaatagg acggatcgga aagaatttcc tgataaccgg
      541 tcatcgcggt attgaaaacg acttcgccag tggtgttgcc tttggcgccg atagactggc
      601 cattgaagat cgttccatct tccagagcca gaaccgcggt tacagtcacg cgggaactcc
      661 cctcgagcct gctcgggccc gataaaaact cgaatttaag tggatgctct ctccgcggag
      721 cgtacatgca caaagcggga ggagttctcg cgaacatcct cccgccctgc gttggactaa
      781 cccgtttcgg attaggcggc aattttacga agcgcgcccc ggggtgtcca cgaaacttct
      841 tgcccgtttc atggcgaaag cgctcggaaa gttctaatga aataaaccga aaacgcttct
      901 aaccggttgt ttttaatcca agtacgttag ccatcgtgta tcggccgggc ttttgcgtcg
      961 caagccagac ggccgccctg atcgctccat ttgcaaagat cgcccggtcg gtggcctcgt
     1021 gaccgaccgt cagccgctcg cccagacccg cgaacagcac tgtatgggta cccacaatgt
     1081 cgcctgcccg gatgctggcg atgccgattt ctccgtcgcg ccgcgcgccc tgcccggccc
     1141 gtcccgacag ggtgtcttca tggggatcga gcccacgagc ccgcgccgca ctttcggcga
     1201 gcgccaatgc ggtacccgaa ggcgcgtcga gtttgtgttt gtggtgagcc tcggaaatct
     1261 cgatgtcgaa ctccgccggc aacgcccgcg ccgcgatgcg caccagctcc tgtgcgaccg
     1321 cgaccccgat cgaagtgtta ggcgccacca gcaccgcgat gtcgcgcgag gccgcggcga
     1381 ttcgcgcgtc gaactcggcg ccgtgcccgg tggtgccgat gacgatgggc acacgcgcgg
     1441 cgcgacagac attcaacgcg cgcaggctca gatcgggtcg cgagaagtct atggccacct
     1501 gggcttttgc gagatcgggc ggtagttcgt tgagcacgga caccccgagc gccttcacgc
     1561 cggcgacttc gcccacgtcg agccccgtcg acttgctgcc cgcggacgcg accgcggcga
     1621 cgactgcaag agttttgccg gccacgctcg cgcgcacgat ggcagtgccc atgcgtccgg
     1681 tggcgccgat caaagctgct gtcgtcacat tgactccctt ttccaactca cgccgacagt
     1741 gtgccccaaa attttatgcg gagcgcgcag tcgtgcgaga ctgccttcca tgatacgcat
     1801 cggcatattg ggcgcggcac ggatcgcgcc acgcggcatc gtgactcctg ccaacgcgct
     1861 gctcggcgcg gaggtggtgg gcatcgcggc gcgcactctc gaacgcgcgc aggacttcgc
     1921 cgcgcagcac tccatcccca tggctttcgg tagttatgcg gagctgatcg cccgcgacga
     1981 catcgacctg gtgtacgtgg cactgccacc gtcggcgcac ctcgagtggt gtacggcggc
     2041 gctcgacagc ggcaaacacg tgctggtcga aaaacccttt gccagcaacg cgcaggatgc
     2101 ggcgcagatg gtggccgccg cacgcgccgc caaccgccac ctcatcgaag ggtttcacta
     2161 ccgcttccac ccgcttttcg aacgcacgct aagcgcattg cgcaatggcg acatcggcaa
     2221 gatccggcac atcgaagcgg tgttcaacgc gaatctcccc gacaccccgg gcgagctgcg
     2281 ctacatcgaa gccctcggcg gcggcgcgtt gatggacctc ggctgctact gcatgcactg
     2341 gatccgcacc gtggctggcg acgaacccac ggtagtcagt gcccgtgccc gctgtgcgac
     2401 gcccggcgtg gacctcgata tcgaagccga cctcgccttc accagcggcc ccacggccac
     2461 gctcaaatgt tcgatgcagc ccgacgacgg cgtgttgtcc cggtacctgc gagtacatgg
     2521 cgacaagggt gtgctcgagc tcgacaaccc ggtgtctccg cattccggct cgacgctgtc
     2581 catcgagtcg ccggattccc ccatgccgca cctggtggcc ggtggcgaca ccacgttcca
     2641 tttccagctg cgccacgtca tcgaagtcat cgaaggccgc gagcagcccc tgaccggcgg
     2701 cgacgatgcc atcggcaaca tgcgcgccat cgacgctatc taccgcgccg cgggattgag
     2761 accgcgcggc ctgaacgcct gacgagcgaa tcgtgggtgc cgcgccaggt ttttcgagca
     2821 agttcgtcct ggcgctcgac gccggcacca cgagttcgcg gtccctgttg ttcgatgcca
     2881 ccggaaaggt ggtagcgctg gcgcagcgcg aattcacaca acatttcccg cgccccggat
     2941 gggtggaaca cgacgccacc gaaatctggg aaacccagcg agccacgatg gccgaggtcc
     3001 tcgaggccgc gcgggcaacg ccgcgcgaca tcgtcgccgt cggtatcacc aaccagcgcg
     3061 agaccacggt gctgtgggat cgcgccacgg gtgcacccct ggcgccggcg atcgtctggc
     3121 aggaccggcg cacggcgccc gcctgcgagc aattgcgcgc cgcgggttgc gagcccgaga
     3181 tcgccgcgcg taccggcctg ctgctcgacc cgtatttttc cgccaccaag ctggcgtggc
     3241 tgctcgacca gatccccggc gcgcgcgctc gcgcggagcg cggcgacctc gcgttcggta
     3301 cggtagatag ctggctgctg ttcaagctca ccggccatcg gcgccatgtc accgacgcca
     3361 ccaatgcaag ccggacactg ctgttcaaca ttcacacggg cgattgggac gaccgcctgc
     3421 tcgaactgct gcgcgtccct cgcgcctgcc tgcccgagtt ggtcgactcg tgcctcgacg
     3481 cgcccgcagc gatcgagatc gacctcgacg gtgtctcact gcccgtgaca ggcatcgcgg
     3541 gcgaccagca ggcggcgctg ttcggacagg cctgtttcgt acccggcatg gccaagaaca
     3601 cctacggcac gggctgtttc gcgctcatga ataccggcgc cgcgccgcaa gcctccaggc
     3661 accgcctcct caccaccgtg gcgtggcgcc gcggcgcaac cacccagtac gcaatcgaag
     3721 gtggcgtgtt catgggcggc gccaccgtgc aatggctgcg tgacgggctc ggcatgatca
     3781 cgagctccga tgaagtcgag gcgctggcgg ccagcgtgcc ggacaccggc gatgtctacc
     3841 tggtgcccgc ctttgccggc ctgggcgctc cgcaatggga tgccgcggcc cgcggtgcca
     3901 tcgttggcct cacgcgcggc accactcgcg cgcatatcgc gcgcgcggcg ctggaaagca
     3961 tcgcgtttca gaccgcggat ctcatcgaag cgatgcaaca ggatgccggc cggccgttgt
     4021 cggagctgcg tgtcgacggc ggcgcggcgc gcaacgacct gctgctccag ttccaggcgg
     4081 acttgttagg cgtgccggta cttcgtcctg tcaacaccga gaccaccgcc tcgggcgcgg
     4141 ccgcgctcgc gggcctcggt gccggcctgt ggcagtcgca gcaggaactc gcggcgttgt
     4201 ggaaactgga gaaacgcttc gaaccgcgca tgacgcgcga cgaggcggga cggcgtcgcg
     4261 cgcgctggag tcaggcggtg gagcgatccc gcgactgggc gaacgcatga cgagccggaa
     4321 acacgcaagg tccgctttgc tggccatctt cgcggtaacg acgctgacgg ttggctgggt
     4381 tctctatcaa cgcgaaggcg aaccccgcta tctcaacgcc agccgcgccg aattcgtcgg
     4441 gctgttcgcg tcaccccctt cgccggattc gccgcagacg cgcgcggaac tcgacgtcct
     4501 gcttgcgatt cagcgcaatc gcaccgcgaa agacgtggac gccgcacgcg cggaccgcaa
     4561 gaccgagatt tctcaattcg cgggagcact gggtctgacc cccgcgcagg tgcgctctct
     4621 gccggccctc gatgcgctcg cggaacaggt ggaagacgac attcgcccgt atgtccgcac
     4681 ggccaagcac cgcttcacgc gattgcggcc ctatgaagtg gactcgcgtc tcgagccctg
     4741 catcggcaac gtccgcgatg acctgtccta tcccagcggc catgccactt tcggcttcgt
     4801 ggtggcctat ctattggcgg acatggtgcc cgagcgccgc cgtgagctga tcgatcgcgc
     4861 cacggagttc gcgcgccagc gcgcggtttg cggcgtgcac ttcccgagcg atatcgaagc
     4921 gggaaacaag gcggccgtat ggctggccca gcacttcctg gcgagcgagg cctatcgaaa
     4981 ggccgtggtg ccggcgcgca atgaactccg cgccgccatg cgccttcctg cgcgctgacc
     5041 tcaacctctc gggctgcggt cagctcggtt cggaagaaaa gaagcgcttt acgccttcga
     5101 agaagctttc gccgcgcggc gcatgtttgc c
//
LOCUS       DATDEU010000014         5552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1315479, whole genome shotgun sequence.
ACCESSION   DATDEU010000014 DATDEU010000000
VERSION     DATDEU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315479"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VIV63_00235"
     CDS             complement(<1..705)
                     /locus_tag="VIV63_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEY6123048.1"
                     /translation="MNTTITTPTRWDALDVLRGLTIMLMLLNLSPGSWEHNYGWLTHA
                     TWEGWALIDVVAPAFLFCIGAAMPLSLQRRSQKGASRRDLVRHVLWRSVLLIAIGFFL
                     NLYPTFDFETVRIPGVLQRIGLCYGMVGMFMLMTARVENGRALELRPSLIAGVAVFIL
                     VSYWALLYFVPVPGYGAPRFDAVGSWPPVIDRAVIGVEHFFPHYKIDGQVVFDPEGIL
                     STWPACFNVLLGALAGI"
     gene            966..2129
                     /locus_tag="VIV63_00240"
     CDS             966..2129
                     /locus_tag="VIV63_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6123049.1"
                     /translation="MSRGLLTGIIAGAVVVIAALAFIFFRPKPTEVTTVVAEAESSGP
                     SLGNAVLNASGYVVARRMATVSSKVTGKIEEILVEEGMVVKKDQVLARLDPVNLKTVL
                     TMSERELDASRRNLDEIEVRLAEARRNLERNESLVQQQLISQTALDASRAEANALAAR
                     LEASKAQVKVAESGLAMRRIDYDDLEVKAPFAGVIISKDAQPGEIVSPMSAGGGFTRT
                     GIATIVDMDSREVEVDVNESYINRVKPEQRVEATLDAYPDAPIAAHVINIVPTADRTK
                     ATVKVRIAFDALDARILPDMGIKVRFLDDAPAPAANAGPRIRVPASAVQREGDNAYVW
                     VVSDGRVERRAVTVGAENNGSIEVRAGVASGDQLVSPVVQGLEDGGKVKLKAS"
     gene            2147..2863
                     /locus_tag="VIV63_00245"
     CDS             2147..2863
                     /locus_tag="VIV63_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6123050.1"
                     /translation="MAAAADTMKNEDPVVVKLRGVAKQYQRGAEVVPVLEKLDLDLPR
                     GDFVALMGPSGSGKTTLLNLIGGLDRPTTGSIDVNGVSIDRLSNAALGRWRADNVGFV
                     FQMYNLLPVLTAERNVELPLLLTKLGKSDRLKRVHTALSLVGLADRMKHKPRELSGGQ
                     EQRVGIARAIVTDPTLLLCDEPTGDLDRKSGDEVLDLLQALNKQHGKTIVMVTHDPRA
                     AERANRVLHLEKGRLEREVK"
     gene            2864..4024
                     /locus_tag="VIV63_00250"
     CDS             2864..4024
                     /locus_tag="VIV63_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6123051.1"
                     /translation="MSWISFVWANITRRKLRLVFTLLIIVLAFLMFGMLDALRTSLSK
                     AVNLTGADRLISISKVSIIDSFPLSHYSKVQAVQGVSSVVHLNWFGGVYKDGKAQIPV
                     FPMNVDEFFKVYPEVKISPEEFAAWKSDRQGMVIGQVLAETYGWKKGDRVPIKSSIFR
                     KANGGDTWEFNIVAIYKVENSAGWDNQSAMFHYDYYNESLQFGRDQIGWMTVKVSNPD
                     ESEAVAKRIDSLFANSSDETKTATEKAFTKQFMAQLGNIGKILVSVVFAVFFTMLLVT
                     ANTMAQSVRERTNEIGVLKTLGFSSGAVLRLIVGEAVFLTVFGGLIGFGLAYLAVGGM
                     ASFMKKYFPVFEIGQSTIVTALVLMVALGVVTGLWPAVSAMRLKITDALRRG"
     gene            4028..5236
                     /locus_tag="VIV63_00255"
     CDS             4028..5236
                     /locus_tag="VIV63_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6123052.1"
                     /translation="MANSLSQIVAVTAMNVRNIPERWASSLVAVVGIGGVTLVLIAVL
                     SIAAGFKQALELSGSADVAIVLRSGATNEMSSGFGLNQVTLIAEAPGIKKDDKSKPIY
                     SAELYVLTEGKLKNKDADANSANLPFRGVSPSAPLLRKSFKIEEGRMMREGTNEILVG
                     DGVASNYENISVGKKVRWGNADWEIVGRFSDDGGIAESEAWADARLVQQVWNRGTSFQ
                     SVRVRLTDDSDATLKKLKDELTKNPQLQVSVTREPQFYAEQQEMMSTLITGAGVIFAI
                     LMGLAAILAAVNTMLNAVASRVREIATLRAMGFGAAPVVFSVIIEALVLGAAGGLLGG
                     VLALLLLNGMQSSTMNFQTFSQITYAFTVTPGLMVVGIVYGLVLTFVASILPGIRAAR
                     MPITSGLREL"
     gene            5239..>5552
                     /locus_tag="VIV63_00260"
     CDS             5239..>5552
                     /locus_tag="VIV63_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123053.1"
                     /translation="MLSRIARACSLGLAIAVSSSAFAQSSPSDWGDIEGRIQYAYYTN
                     DARALNSVLASLKPHLAEGETETGEGAVIRAYFRALTHYRLAQVLSSTKKGDAKDAID
                     ECG"
BASE COUNT         1081 a         1717 c         1778 g          976 t
ORIGIN      
        1 gatgcccgcc agcgcgccga gcaacacatt gaaacaggcg ggccaggtcg acaggatccc
       61 ttccggatcg aacacgacct ggccgtctat cttgtagtgc ggaaagaagt gctcgacgcc
      121 gatcaccgcg cgatcgatga ccggcggcca gcttccgacc gcgtcgaaac gcggcgcgcc
      181 gtacccgggc accggtacga aatagagaag ggcccagtac gaaacgagga tgaacaccgc
      241 cacgcccgcg atcagcgacg gtcgtagttc gagcgcgcgg ccgttctcga cgcgcgccgt
      301 catcagcatg aacatgccga ccatgccgta acacaggccg atgcgttgca ggacgcccgg
      361 aatgcgcacg gtttcgaaat cgaacgtggg atagaggttg aggaagaatc cgatcgcgat
      421 cagaaggacg ctgcgccaca acacatgccg cacgagatcg cgtcgcgacg cgcctttctg
      481 tgagcgccgc tgcaacgaca gcggcatcgc ggcgccgatg cagaacagga atgccggcgc
      541 cacgacatcg atgagcgccc agccttccca ggtcgcatgt gtcagccagc cgtagttgtg
      601 ctcccaggag ccggggctca ggttgagcag catcagcatg atggtgaggc cccgaagtac
      661 gtcgagcgcg tcccatcgag tgggtgtcgt tattgttgtg ttcattcgat gcgcccgggg
      721 gcgcggattc taacccgcga caaaaggcaa acaattcccg tcgcatcgtc gatttacagt
      781 gtccacattc gtcaactact ggacatgaca catgacagat gtgtcccaat ccgatttcca
      841 ttaatcttca gcccggtcga tatcgcctca atggtttcag ggacttaaat caccgcatgc
      901 ctctcgatac cgaatcactg ggttcgctgc gaatcgaacg ttccgcgccc tcgtctggag
      961 gcggcatgag ccgcggtctg ctcaccggca tcatcgcggg tgcggtggtg gtcattgcgg
     1021 cgctcgcctt catcttcttc cgtcccaaac ccaccgaagt caccaccgtg gtcgccgaag
     1081 cggaatcgag tggcccgagt ctgggaaacg ccgtgctcaa tgcgtccggc tacgtggtgg
     1141 cgcgtcgcat ggccacggtg tcgtccaagg tcaccggcaa gatcgaagag atcctggtcg
     1201 aagaaggcat ggtggtgaaa aaggaccagg tgctggcgcg gctcgatccg gtgaatctca
     1261 agaccgtgct cacgatgtcg gagcgtgaac tggacgcctc gcggcgcaac ctcgacgaga
     1321 tcgaagtgcg tctcgccgaa gcccgccgca atctcgagcg aaacgaatcc ctcgtgcagc
     1381 agcaactcat cagccagacc gcgctcgacg cgtcgcgtgc cgaggcgaat gcgctggcgg
     1441 cgcgcctcga agcatcgaag gcgcaagtca aggtggccga gagcggactt gcgatgcgcc
     1501 gcatcgacta cgacgacctc gaggtcaagg cgccgttcgc gggcgtcatc atctccaagg
     1561 acgcgcagcc cggcgaaatc gtgtcgccga tgtccgcggg tggcggcttc acgcgtaccg
     1621 gcatcgccac catcgtcgac atggactcac gcgaagtcga agtggacgtc aacgagtcgt
     1681 acatcaaccg cgtgaaaccc gagcaacgcg tcgaagcaac gctcgacgcg tatcccgatg
     1741 cgccgattgc ggcccacgtc atcaacatcg tgcccacggc cgatcggacg aaagcgacgg
     1801 tcaaggtgcg aatcgcgttc gacgcgctcg acgcgcgcat cctgcccgac atgggcatca
     1861 aggtccgttt tctcgacgat gcgccggcgc cggccgccaa cgccgggccg cgcatccgcg
     1921 tgccggcgtc cgcggtccag cgtgaaggcg acaatgccta tgtgtgggta gttagcgatg
     1981 gccgcgtgga acgccgcgcg gtgacggtgg gtgcggagaa caatggcagc atcgaagtgc
     2041 gtgccggcgt cgcgagcggt gaccaactgg tgtcgccggt ggtgcaggga ctggaagacg
     2101 gcggcaaggt aaagctcaag gcaagttgat acacgaggac atgaacatgg cggcagcagc
     2161 agacacgatg aagaatgaag atccggtggt ggtcaaacta cgcggcgtcg ccaagcaata
     2221 tcagcgcggc gccgaggtgg tgccggtgct cgagaagctc gatctcgacc tgccacgcgg
     2281 cgacttcgtc gcgctcatgg gcccgtcggg ctcgggcaag accacattgc tcaacctgat
     2341 cggcggtctc gatcgtccga ccacgggcag catcgacgtg aacggcgtgt ccatcgaccg
     2401 gctgtcgaac gccgcgctcg gccgctggcg cgcggacaac gtcggcttcg tgttccagat
     2461 gtacaacctg ctgccggtgc tcacggccga acgcaacgtc gaactgccgc tgctgctcac
     2521 caagctcggc aagtccgatc gcctgaagcg cgtgcacacg gcgctcagcc tcgtgggcct
     2581 cgccgatcgc atgaagcaca agccgcgcga actctcgggt ggccaggagc agcgcgtcgg
     2641 tatcgcgcgc gccatcgtca ccgatcccac actgctgctg tgcgacgagc ccacgggcga
     2701 cctcgaccgc aagtcgggcg acgaagtgct cgacctgctg caggcgctga acaagcagca
     2761 cggcaagacc atcgtcatgg tcacgcacga cccccgcgcc gccgaacggg cgaaccgcgt
     2821 gctccatctg gagaaggggc gcctcgagcg ggaggtcaag taaatgtcct ggatctcatt
     2881 cgtctgggca aacatcacgc ggcgcaagct gcggctggtg ttcaccctgc tgatcatcgt
     2941 cctcgcgttc ctgatgttcg gaatgctgga tgcgttgcgc acctcgctgt ccaaggccgt
     3001 gaatctcacg ggcgcggacc gtctcatctc catcagcaag gtgtccatca tcgactcctt
     3061 cccgttgtct cactactcga aagtgcaggc cgtgcagggc gtgagttcgg tggttcacct
     3121 gaactggttc ggcggcgtct acaaggacgg caaggcgcag attcccgtgt tcccgatgaa
     3181 cgtcgacgaa ttcttcaagg tgtatcccga ggtcaagatc agccccgaag agttcgcggc
     3241 gtggaaatcc gatcgccagg gcatggtcat cggccaggtg ctcgctgaga cctacggctg
     3301 gaagaaaggc gatcgcgtgc ccatcaagtc gtcgatcttc cgcaaggcca acggcggcga
     3361 cacctgggaa ttcaacatcg tcgccatcta caaggtcgag aacagcgccg gctgggacaa
     3421 ccagagcgcc atgttccact acgactacta caacgaatcg ttgcagttcg gccgtgacca
     3481 gatcggctgg atgacggtga aggtgtcgaa cccggacgaa tcggaagcgg tcgcgaagcg
     3541 tatcgactcc ctgttcgcca attcctcgga cgagaccaag accgcgaccg agaaggcgtt
     3601 cacgaagcag ttcatggcgc agctcggcaa catcggcaag atcctcgtct cggtggtgtt
     3661 cgccgtgttc ttcacgatgt tgctggtgac ggccaacacc atggcgcaat cggtgcgtga
     3721 gcgcaccaac gagatcggtg tattgaagac gctgggtttc agctcgggtg cggtgctgcg
     3781 gctgatcgtc ggtgaggccg tgttcctcac cgtcttcggc gggctcatcg gattcggcct
     3841 ggcctatctc gcggtgggcg gcatggcgag cttcatgaag aaatacttcc cggtgttcga
     3901 gatcgggcag agcacgatcg ttaccgcgct ggtactcatg gtcgcactgg gtgtcgtcac
     3961 cggtctgtgg ccggcggtat cggccatgcg gttgaagatc accgacgcgt tgcgcagggg
     4021 ttgagtcatg gcaaattcct tatcccagat cgtggcggtc accgcgatga acgtgcgcaa
     4081 cattccggag cgctgggcat cgtcgctggt cgcggtcgtc ggcatcggcg gcgtcacgct
     4141 ggtgctgatc gcggtgttgt cgatcgcggc gggcttcaag caggcactcg agctgtccgg
     4201 ttcggcggac gtggcaatcg tgttgcgctc gggcgcgacc aacgaaatga gttcgggttt
     4261 cggcctgaac caggtcacgc tgatcgccga ggcgccgggc atcaagaagg acgacaagag
     4321 caagcccatc tactccgccg agttgtacgt gctcaccgag ggcaaactca agaacaagga
     4381 tgcggatgcc aattccgcga acctcccgtt ccgtggcgta tcgccatcag cgccgctgct
     4441 gcgcaagtcc ttcaagatcg aagaaggccg catgatgcgc gaaggcacga acgagatcct
     4501 ggtgggggac ggcgtcgcat ccaactacga gaacatctcg gtcggcaaga aagtgcgctg
     4561 gggcaatgcc gattgggaaa tcgtcggacg tttcagtgac gatggcggta tcgccgagtc
     4621 cgaggcctgg gccgatgcgc gcctcgtgca gcaggtctgg aatcgcggca cgagctttca
     4681 gtccgtgcgc gtgcgcctca cggacgattc cgatgcgacg ctcaagaaac tcaaggacga
     4741 actgaccaag aatccgcagc tgcaggtgtc cgtcacgcgc gagccgcagt tctacgcgga
     4801 acagcaggag atgatgtcca cgctcatcac cggtgcgggc gtcatttttg caatcctcat
     4861 gggtctcgcg gccatcctcg cggcggtcaa caccatgctc aatgccgtgg cgagccgcgt
     4921 gcgtgaaatc gccacgctgc gcgccatggg gttcggtgcc gcgcccgtgg tgttctccgt
     4981 catcatcgaa gcgctcgtgc tgggcgccgc gggtggattg ctcggtggcg tgctggcgct
     5041 gctgctgctc aacgggatgc agagctcgac gatgaatttc cagacgttca gccagatcac
     5101 ctacgcgttc acggtcacgc ccgggctcat ggtcgtcggc atcgtctacg gactggtgct
     5161 gacgttcgtc gccagcatcc ttccggggat tcgcgccgcg cgtatgccca tcaccagcgg
     5221 acttcgcgag ttgtgagggt gttatcgcgg atcgcgcgcg cctgttcgct gggcttggcg
     5281 atcgccgtgt cgtcgtcggc gttcgcccag tcatcaccat cggactgggg cgatatcgag
     5341 gggcgtatcc agtacgccta ctacaccaac gacgctcgcg cgctcaacag cgtgcttgcc
     5401 tcgctcaagc cgcacctggc agaaggcgag accgagaccg gcgagggcgc ggtgatacgc
     5461 gcctatttcc gggcgctgac gcactaccgc ctggcgcagg tcctctccag cacgaagaag
     5521 ggcgacgcca aggacgccat cgacgaatgc gg
//
LOCUS       DATDEU010000015         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1023821, whole genome shotgun sequence.
ACCESSION   DATDEU010000015 DATDEU010000000
VERSION     DATDEU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023821"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VIV63_00265"
     CDS             complement(<1..353)
                     /locus_tag="VIV63_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123054.1"
                     /translation="MKQALSGNRLQSISLHDERGDVLWLDEGALGPDEHNLVIEAAAA
                     LTAGPELTYVFQNIGDGRAAAFLPARSPLNDLLGIAMIIADAKFLDSRGAAKFVTPTT
                     TNLMRRLSFLRKPTAP"
     gene            697..>2365
                     /locus_tag="VIV63_00270"
     CDS             697..>2365
                     /locus_tag="VIV63_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY6123055.1"
                     /translation="MRISASALLRVCLLASVPICASAQTEPVVFEAESGTLGANIATG
                     VDGAVTFVTTTVNTTTPPSAPHIGTYSVTFPAAGNYELYARYSVGPNAANDDSWYFGQ
                     GFGVKTPEVGAQWALQNEAGTGFTNPASVVLVGGSAGSNVFKWVKITGAQGPAAWVVP
                     ADSLTQTFQFGTREDGMRMDKFAFGRPGVCYTVNDLNTGGPATGTCPPPPPPDPPPYT
                     RTAPPIATGLDKYLGAAWSPGTASLNFANYWNQVTPENGGKWGTVEGTRDVMNWAQAD
                     EAYALAKANGFPFKWHTFIWGNQQPAWIESLSTAEQREEIEEWYAAVAARYPEIDQIE
                     VVNEPLHDPPRGPTNGNYIEALGGNGVTGWDWIITSFQLARQYFPNAELILNDYSITN
                     DGNATTNYITIINLLKERGLIDAIGDQAHAFSTTEAAPMNTHKNNLNRLAATGLPIYI
                     TELDIDGVLAGVVNDPVQVGNFQRIFPTFWEHPAVKGVTIWGYVRGFHWRNAQGDWLL
                     YPNGGERPALQWLIRYVQNAPAVVGPQTLSVNESAAGGTPVGAVLASD"
BASE COUNT          437 a          759 c          769 g          400 t
ORIGIN      
        1 ggggccgtcg gcttgcgcag gaacgaaagc cggcgcatga gattcgtggt cgtcggcgtg
       61 acgaattttg ccgcgcccct gctgtcgagg aacttggcgt cggcgatgat catcgcgatg
      121 ccgagaagat cgttgagcgg cgaacgggcc ggcaggaacg ctgccgcgcg gccatcgccg
      181 atgttctgga atacgtaagt gagttcgggt ccggcggtca gtgccgcggc cgcttcgatg
      241 acgaggttgt gttcgtcggg gcccagcgcg ccttcgtcga gccacaggac gtcgccgcgt
      301 tcatcgtgaa gggaaatcga ttgcagccga ttgcctgaaa gcgcttgctt caacgcctcg
      361 ccgatcgcgg cctttgaatc gccgctagct gtgacactcg cgctcgcctg cattcgtgaa
      421 aacttccccg ggctgagcat ccagagtcgc aaatcaccga atgcgccgcc gtgacatagc
      481 tcccgttccc cggattgcta gagcggggat ttgacgccgt tatgttcaat cggcggccgc
      541 ttcgcgcagt ggttcacacc actaccaaag tcgaagggac atcggcgcgg gattcgttgc
      601 ggttggtacc gctaccgatt tcgtttttgt tgggctagat tcgggccgcg agacagtggc
      661 taaccactgc gtttcaaaac gaaggggaaa catcagatga gaatttcagc gagcgcgctg
      721 ctgcgcgtat gtctactcgc gtccgtgcca atctgcgcgt cggcgcagac cgagccggtc
      781 gtgttcgaag ccgagtcggg aacactcggt gcaaatatcg caacgggcgt cgatggcgcc
      841 gtgacgttcg tcaccaccac ggtgaacacg accacgccgc cgagcgcgcc gcacatcggc
      901 acttacagcg tcacttttcc cgccgcggga aactacgagc tgtacgcgcg ttacagcgtc
      961 ggcccgaacg ccgccaacga tgacagctgg tatttcggcc agggcttcgg cgtgaagacg
     1021 ccggaagtcg gcgcgcaatg ggcgctgcag aacgaagccg gtacgggctt cacgaatccc
     1081 gcttcggtcg tcctggtcgg cggttcagcc ggcagcaacg tcttcaagtg ggtgaagatc
     1141 acgggcgcgc agggccccgc ggcgtgggtc gtacccgccg actcgctcac gcagacgttc
     1201 cagttcggca cgcgcgaaga cggcatgcgc atggacaagt tcgcgttcgg tcgcccgggt
     1261 gtctgctaca ccgtgaacga tctgaacacc ggtggtccgg ccacgggcac ctgcccgccg
     1321 ccgccgccgc cggatccgcc gccgtacacg cgcaccgctc cgccgatcgc gacgggcctc
     1381 gacaagtacc tcggcgccgc ctggagcccg ggcaccgcgt cgctcaactt cgctaactac
     1441 tggaaccagg tgacgccgga gaacggcggc aagtggggca cggtcgaagg cacgcgcgac
     1501 gtcatgaact gggcgcaggc cgacgaggcc tacgcgctgg cgaaggcgaa cggcttcccg
     1561 ttcaagtggc acacgttcat ctggggcaac cagcagccgg cgtggatcga gtcgctctcg
     1621 acggccgagc agcgtgagga aatcgaggaa tggtatgcgg cggtggccgc gcgttacccc
     1681 gagatcgacc agatcgaagt cgtgaacgag ccgctgcacg atccgccgcg tggtccgacc
     1741 aacggcaact acatcgaggc gctgggtggc aacggcgtga ccggctggga ctggatcatc
     1801 acgtccttcc agctggcccg gcagtacttc ccgaatgccg agctgatcct gaacgactac
     1861 agcattacca atgacggcaa tgcgaccacc aactacatca ccatcatcaa cctgctcaaa
     1921 gagcgtggct tgatcgacgc catcggcgac caggcgcatg ccttctcgac caccgaagcg
     1981 gcgccgatga acacccacaa gaacaacctg aatcgcctcg cggcgaccgg gctgcctatc
     2041 tacatcaccg agctggacat cgacggcgtg ctcgcgggcg tggtcaacga cccggtgcag
     2101 gtcggcaact tccagcgcat cttcccgacg ttctgggaac atccggccgt gaagggcgtg
     2161 accatctggg gctacgtgcg tggcttccac tggcgcaatg cccagggcga ctggctgctg
     2221 tatccgaacg gcggcgaacg cccggcgctg cagtggctga tccgctatgt gcagaacgcg
     2281 cccgcagtcg tggggccgca gaccttgtcg gtgaacgagt ccgctgccgg cggcacgccg
     2341 gtgggtgcgg tgctcgcctc cgacg
//
LOCUS       DATDEU010000016         8461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2667, whole genome shotgun sequence.
ACCESSION   DATDEU010000016 DATDEU010000000
VERSION     DATDEU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2667"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VIV63_00275"
     CDS             complement(<1..200)
                     /locus_tag="VIV63_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123056.1"
                     /translation="MSATELTGIQQSWEQANYQAADAADKERQLEPDGMDPNYFIGEL
                     LYEKGEYSRSLQHLQKALAAPPR"
     gene            complement(261..2453)
                     /locus_tag="VIV63_00280"
     CDS             complement(261..2453)
                     /locus_tag="VIV63_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123057.1"
                     /translation="MNKKIWMTLMAAVAGTAAAQESTPGQLDEVTVTAERRSENIRDV
                     PNSVSTVSGEDLDVLATGGQDVRLLAGRIPSLNVESSFGRAFPRFYIRGYGNGDFRLN
                     ASQPVSLIYDDVVQENPILKGFPIFDMDQIEVLRGPQGTLFGRNTPGGVVKFDSVEPE
                     PDFGGYFNVSDATYNTANIEGAINIPLGEKWSARVSGLYQHRDDYVGNSLTGDDDIYE
                     GYDDRAARVQLQYEGDTFTALFNAHMRNLDGTARLFRANIIESGSNDLIDGFDADSPI
                     QADGANQQELNIAGGSVRLSWDFGNLALYSITGYESMDAYSRGDIDGGFGCGFCSLPN
                     GPGFIPFPSETADGIPTLDQFTQEVRLESQLDGALNWQAGLYYFDEDYDTEFFSYDSL
                     GGGAQVQYLRANQTNTAWAVFGSITYDVTDAFELRAGVRYTEDEKDFVTGTPEGFTFP
                     DPSLPTSASLSDDNVSWDLAATFALNEDVNLYARVATGFRGASVQPAGAFGNQSVAEP
                     ETNTSYELGVKADLMDRRAKLYFNVFHYEVKDQQLTAVGGTNNAVRLLNAAKTVGQGA
                     ELDFQAYLTSNLLVTLGASYNDTEIEDDSISVGTCFACTVTDPIVGGFAVIDGNQLPQ
                     APEVIANFTLRYGFPVGNGELFAYTDWSYRSEVNFFLYESIEFTGKSLLEGGLRVGYN
                     WSDEKYEAAIFGRNITDEVVAVGAIDFNNLTGFVNEPQIWGVQFKARF"
     gene            complement(2552..3022)
                     /locus_tag="VIV63_00285"
     CDS             complement(2552..3022)
                     /locus_tag="VIV63_00285"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6123058.1"
                     /translation="MATRSTRQIVPHLWYIKEANEAARFYVKLFPKSRIDHTWSLMGG
                     TVKCVEFKLFGQPFFAMSAGPHDAFNDAISFGVYCRNQKEVDRYWNAILKNGGKAVAC
                     GWIRDKFGVRWQIVPTVLMEMMGDRDKKKAARVAAEMQKQVKFDVASLKEAFKG"
     gene            complement(3041..3841)
                     /locus_tag="VIV63_00290"
     CDS             complement(3041..3841)
                     /locus_tag="VIV63_00290"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEY6123059.1"
                     /translation="MSALRDFIEAHPRLAVLTGAGVSTGSGIPDYRDEQGEWKRARPV
                     EFRPFMTDARVRQRYWARSTVGWPIISGAKPNGAHLALARLEADGFVRVLITQNVDGL
                     HATAGSRNVIDLHGRIDSVRCMSGGHDMPRAELQRRLLSSNPTWAEIEGRVAPDGDVD
                     IDGRDYSGFIVPDCPECGGILKPDVVFFGEPVPRERVDRAFEGVAQADALLVVGSSLM
                     VYSGFRFAEAAAAAGKPVAAVNLGRTRADHLLTLKISEPCDEALAEAI"
     gene            3885..5042
                     /locus_tag="VIV63_00295"
     CDS             3885..5042
                     /locus_tag="VIV63_00295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6123060.1"
                     /translation="MTADTPRTRLATRIAFFVAGFAISCWAPLVPYAKDRVAVDERML
                     GILLLCLGIGSLLAMFFTGMLSARFGTRPVILGSSWGLCATLPFLALAPTPFTLGLTL
                     LFFGASLGSLDVSVNIHAVEVERDAGQPLMSGFHAQYSLGGFAGAMFITLLLSAALSP
                     FVCVAIASALMLAMISVASPSLLRTRAADGEPHFALPRGVVLVIAILAAICFLAEGAV
                     LDWGALLITGNGLVNVEQGGIGFVLFSVAMTLMRFCGDALTARFGDRPTLIWGGLVAM
                     AGFAVLLLAPVAWVAMMGFVLIGLGAANIVPVLFRRAGSQTVMPAGLAIAAITTMGYA
                     GILLGPAAIGFVADHVGLTMAFWMIPAILALVPLCAAVVVKTVTVRERASV"
     gene            5052..6083
                     /gene="mltB"
                     /locus_tag="VIV63_00300"
     CDS             5052..6083
                     /gene="mltB"
                     /locus_tag="VIV63_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEY6123061.1"
                     /translation="MKSPAKILSSFLLAACLLAVQPVAGSDYLSRPDVRAFIKSMSEE
                     HGIDKSELRRVLGDARYTPVAVRLIGPEPSSAPSPARSYSRYRAKFLTPELIADGSRF
                     WSRHAAALGRAEAEFGVPAEVILGILGVETAYGRNTGSFRAIDALATIAFDGVRRQEY
                     FRDELKELLLLARDTGIDPLAIKGSYAGAVGLPQFMPSSYRRYAVDFDRDGAIDLVGS
                     PSDAIGSIASYMKAFGWVTGEPPTAPVRLAPGSETDLVSGLQRVHDQLEVQGKGVVFS
                     GRDPPAGLVSIFELPTPGKPSKFVAGFTNFEVVTRYNRSTFYASAVLELADAIQKAHD
                     RMHMASADR"
     gene            6146..6613
                     /locus_tag="VIV63_00305"
     CDS             6146..6613
                     /locus_tag="VIV63_00305"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6123062.1"
                     /translation="MKNPSRVAAVLVVQFLLAAVAPLQAKDAGSKIPADLAARVHAYD
                     EAQVKGDKAALEDLVADDYVLMNSRGQRQTKTDLIRDYTKPGFKLEPFTIEEPVELVW
                     SDGAVMGGVADLRGVDDGQAFTLRLRFSDIWAKRGGKWRVIYTHVNRETVSAQ"
     gene            6770..8143
                     /locus_tag="VIV63_00310"
     CDS             6770..8143
                     /locus_tag="VIV63_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEY6123063.1"
                     /translation="MQSAGAGELLDRAVLPANTFSTGPTSGQFASGANGNILPLINKQ
                     PVQGFSAVLRGPVKGTYLVMSDNGFGNKANSPDALLRVNAVKPDFETGDVKPVNRFTG
                     DELNDFTPDSFMTLRDPWKKVPWPIVADGVNYPGTPTGGGAAIPVDASIKAGRLLTGA
                     DFDIEAFRRAPDGTFWFGDEFGPFLIHTDSFGRVLEAPIPLPNFRGLPSAAGGSEVNP
                     LVQAVSNPIRTLAANLPDSGGFEGMALNKSGTRLYALLEKAINGDPVRTRLIISEFSL
                     GQRKYTGKTFAYPLDAPSNAIGDFTALTDREYIIIERDGGQGDASDPRFTNPARFKKI
                     FRIDLDEVDASGTLIKEEVVDLMNIYDPRDVAEDGGTKTVFTFPFTTIEDVLVLDNNR
                     LLVIDDNNYPGSAGREFGVSDNNEFIVVHVAPLLDCEEDEGHGHGKHGKSHRKHHDKS
                     ECELARD"
     gene            8205..>8461
                     /locus_tag="VIV63_00315"
     CDS             8205..>8461
                     /locus_tag="VIV63_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY6123064.1"
                     /translation="MKPPTLDQLRRYAIARSLFKPTKLSKAIDKLGFVQADPIRAPAR
                     AQDLMLRHRVTGYRVGDLERRYPRLPIEEDFFVNYGFMPREL"
BASE COUNT         1464 a         2662 c         2669 g         1666 t
ORIGIN      
        1 cgtggcggcg ccgccaacgc cttctggaga tgttgcagcg aacgggagta ctcccctttc
       61 tcgtacaaca attcgccgat gaagtagttg gggtccatgc cgtccggttc gagctgacgc
      121 tctttgtccg ccgcatccgc ggcctggtag ttggcctgct cccagctttg ctggatgccg
      181 gtcagttcag ttgcgctcac gggacttaga cgtcccgtgc ccccgaaacc cgtcgcgcgg
      241 gaatcagatt gtcgtcgagc tcagaacctc gccttgaact gcacacccca gatctgtggc
      301 tcgttcacga agccggtcag attgttgaag tcgatggcgc cgacggcgac aacctcatcc
      361 gtgatgttgc ggccaaagat cgcggcctcg tatttctcgt cgctccagtt gtagcccacg
      421 cgcagtccgc cctcgagcaa cgacttgccg gtgaactcga tggactcgta gaggaagaag
      481 ttcacttcgc tgcgataaga ccagtcggtg tacgcgaaca attcgccgtt gccgacgggg
      541 aagccgtaac gcagcgtgaa attcgcaatc acctcgggcg cctggggcaa ctgattgccg
      601 tcgatcacgg cgaagccgcc cacgatgggg tcggtgacgg tgcaggcaaa gcaggtgccc
      661 accgagatgc tgtcgtcttc gatctcggtg tcgttgtagc tcgcgcccag cgtcaccagc
      721 aggttggatg tcagataggc ctggaaatcg agctccgcgc cctggcccac ggtcttggcc
      781 gcattcaaca gacgcacggc gttgttagtg ccgccgacgg cggtgagctg ctgatccttc
      841 acttcgtaat ggaacacgtt gaagtacagc ttcgcgcgac ggtccatcag gtcggccttc
      901 acgccgagtt cataggaagt gttcgtctcg ggttcggcca ccgactggtt gccgaaagcc
      961 ccggccggct gcacgctggc gccgcggaag ccggtggcga ctcgcgcgta taggttgacg
     1021 tcctcattca gagcaaacgt ggcggcgaga tcccagctca cattgtcgtc gctcaagctg
     1081 gcgctggtgg gcagggacgg atcgggaaac gtgaatcctt ccggcgtgcc ggtgacgaaa
     1141 tctttttcgt cttcggtgta acgcacgcct gcacgcaact cgaatgcgtc agttacgtcg
     1201 taagtgatcg aaccgaacac tgcccacgcc gtgttggtct ggttggcgcg caggtattgg
     1261 acttgcgcgc cgccgccgag actgtcgtac gagaagaact cggtgtcgta gtcctcatcg
     1321 aagtaataga ggcccgcctg ccagttgagc gcgccgtcga gttgcgattc gaggcgcact
     1381 tcctgcgtga actgatcgag ggtcggaatt ccatccgcgg tttcgctcgg gaacgggatg
     1441 aagcccggac cgttcggcag gctgcagaac ccgcagccga agccgccgtc gatatcgccg
     1501 cggctgtacg cgtccatcga ttcgtaaccg gtgatcgagt acagcgcgag attgccgaaa
     1561 tcccagctca gccgcacgct gccgccggcg atgttcagtt cctgctggtt ggcgccgtcg
     1621 gcctggatgg ggctgtccgc atcgaagccg tcgatcagat cgttgctgcc ggactcgatg
     1681 atgttggcgc ggaacaaacg cgcggtccca tcgaggttgc gcatgtgtgc gttgaacaac
     1741 gcggtgaagg tgtcaccttc atactgcaac tgcacacgcg ccgcgcgatc gtcataacct
     1801 tcgtagatgt cgtcatcgcc ggtgagcgag ttgccgacgt agtcgtcgcg atgctggtag
     1861 agccccgaca cgcgcgcgga ccatttctcg ccgagcggga tgttgatggc gccttcgatg
     1921 ttcgcggtgt tgtaggtcgc gtcggacaca ttgaaatagc caccgaaatc aggctcgggc
     1981 tcgacggagt cgaacttgac cacgccgccg ggtgtgttgc ggccgaacag cgtgccctgg
     2041 ggtccgcgca gcacttcgat ctgatccatg tcgaagatgg ggaaaccctt gaggatggga
     2101 ttttcctgca cgacgtcgtc atagatcagc gagacgggct gcgatgcgtt gaggcgaaaa
     2161 tcgccgttgc cgtagccgcg aatgtagaag cgcgggaagg cgcggccgaa tgacgattcg
     2221 acgttgaggc tggggatgcg cccggccagc aggcgtacgt cctggccgcc ggtggcgagt
     2281 acgtccagat cttcgccgct cacggtcgac accgaattcg gcacgtcgcg gatgttttcg
     2341 gatcggcgtt ccgcggtgac ggttacttcg tcgagctggc ccggtgtcga ttcttgcgcc
     2401 gcagcggttc cagcgacggc ggccatcaaa gtcatccaga tcttcttgtt catgaggctc
     2461 cccaattccg agtttcgcgg agcatacatc tctatttaat ggctagtcac cgcgcaccaa
     2521 accggatcgc tcgggcgctt gcggcaccat ttcagccctt gaatgcttcc ttgaggctgg
     2581 cgacgtcgaa cttcacctgc ttctgcattt cagcggcaac ccgtgcggct ttcttcttgt
     2641 cgcggtcgcc catcatctcc atgagtacgg tgggaacgat ctgccatcgc acgccgaact
     2701 tgtcgcggat ccagccgcac gcgacggcct tgccgccgtt cttgaggatc gcgttccagt
     2761 aacgatcgac ttccttctgg ttgcggcaat acacgccgaa cgagatggca tcgttgaagg
     2821 cgtcgtgcgg gcctgcgctc atcgcgaaga aaggctggcc gaacaatttg aactcgacgc
     2881 acttgacggt cccgcccatc agcgaccagg tgtgatcgat gcgtgacttg ggaaacagct
     2941 tgacgtagaa ccgcgccgct tcgttcgcct ccttgatgta ccagaggtgc gggacgattt
     3001 gtcgggtgga gcgagtggcc atgaatttcc tccaggtgaa tcagattgct tcggcaagcg
     3061 cttcatcgca cggttcactg atcttcaatg tcagcaagtg atccgcgcgc gttcgcccga
     3121 gattgacggc ggcgacgggt ttgccagccg ccgcggcggc ctcggcaaac cggaaaccgg
     3181 aatacaccat gagcgacgat ccgacaacga gcaacgcgtc tgcttgcgcc acgccttcga
     3241 acgcgcgatc gactcgctca cgcggcacgg gttcgccgaa gaacacgacg tcgggtttga
     3301 ggatgccacc acattcggga cagtcgggga caatgaaacc gctgtagtcg cgcccatcga
     3361 tgtcgacgtc gccatcgggc gcaacgcgtc cttcgatctc cgcccacgtg ggattgctcg
     3421 aaagcagacg ccgctgtagt tcggcgcgcg gcatgtcgtg accgcctgac atgcaacgca
     3481 cactgtcgat ccgcccgtgc aggtcgatca cgttgcggct gccagcggtt gcgtgcagcc
     3541 cgtcgacgtt ttgcgtgatc aggacccgaa cgaatccgtc ggcttcgagg cgcgcgagtg
     3601 cgagatgcgc gccgttgggc tttgcaccgc tgatgatggg ccaacctacc gtgctgcgcg
     3661 cccagtagcg ctggcgcacc ctggcgtccg tcatgaacgg gcgaaattcc acgggccgcg
     3721 cgcgtttcca ttcgccttgc tcgtcgcgat agtcgggaat gccggagccc gtgctgacgc
     3781 ccgcgccggt gagcaccgcg aggcgtggat gggcctcaat gaaatcacgc agtgcgctca
     3841 tcttctgtcc ttcacccgcc gggaggccgt agtatcgccg cgccttgaca gcagatacac
     3901 cacgaacgcg cctcgccact cgcatcgcat tcttcgtcgc gggattcgcc atatcctgtt
     3961 gggcgcctct cgtgccctac gcgaaggatc gcgtcgcggt cgatgagcgc atgctcggca
     4021 tcctgttgct gtgcctcggc atcggctcgc tgctggcgat gttcttcacg ggcatgttga
     4081 gcgcgcggtt cggaacgcgt cccgtcatcc tgggcagcag ctggggattg tgcgcgacgc
     4141 tgcctttcct ggcgctcgcc cccacgccat tcactcttgg actgacgctg ctcttcttcg
     4201 gcgcctcgct cggctcgctc gacgtgtcgg tgaatattca cgcggtcgaa gtggagcgcg
     4261 acgccggtca gccgctgatg tcgggattcc acgcgcaata cagcctgggc gggttcgcgg
     4321 gcgcgatgtt catcacgctg ctgctatccg cggccttgag tcctttcgtc tgcgtggcga
     4381 ttgcgtcggc tctgatgttg gcaatgatct ccgtggcgtc gccgagcctg ctacgaaccc
     4441 gggccgcgga tggtgaaccg cacttcgcat tgccgagggg cgtggtgctg gtcatcgcca
     4501 tactcgccgc gatctgcttc ctggccgaag gcgcggtgct cgactggggc gcgttgctca
     4561 tcaccggcaa tggactcgtg aacgtggagc agggcggcat cggcttcgtg ttgttctcgg
     4621 tggcgatgac gctgatgcgg ttctgcggcg atgcgttgac cgcgcgcttc ggtgatcgac
     4681 ctactctgat ctggggcggg ctcgtcgcca tggccggatt cgcggtgctg ctgctggcgc
     4741 cggtggcgtg ggtcgcgatg atgggcttcg tgttgatcgg gctcggcgcg gcgaacatcg
     4801 tgccggtgct attccgccgg gccggaagtc agacggtcat gcccgcgggt ctggcgatcg
     4861 cggcgatcac cacgatgggt tacgccggca tcctgctggg tccggcggcc atcggattcg
     4921 tggccgatca tgtgggactc acgatggcct tctggatgat tccggcaatc ctggcactgg
     4981 tgccgttatg cgctgccgtc gttgtgaaaa ccgtcaccgt tcgcgagcgt gcatcggttt
     5041 aactttgctg ggtgaaatcc cccgcgaaga tcctgtcgtc gttcttgctc gccgcgtgcc
     5101 tgctcgcggt gcagcctgtt gcgggctcgg attacctctc acgccccgat gttcgtgcgt
     5161 tcatcaaatc gatgagcgaa gaacacggca tcgacaaatc ggagctccgg cgggtcctgg
     5221 gcgacgcgcg ctacacgccc gtggcggtgc gcctgattgg cccggagcca agctcggctc
     5281 cttcgccggc gcgttcgtac tcgcgttatc gcgccaaatt cctgacgccc gaactgatcg
     5341 cggacggttc ccgcttctgg tcgcgtcacg cggcagctct tggccgcgcc gaagcggaat
     5401 tcggcgttcc ggccgaggtg atcctgggga tcctcggagt cgagaccgcc tatggccgca
     5461 acacgggctc gttccgtgcc atcgacgcgc tggcgaccat cgccttcgac ggcgtgcgtc
     5521 gccaggaata tttccgtgac gaattgaaag aactcctgct gctcgcgcgc gacacgggta
     5581 tcgatccgct cgccatcaag ggctcgtatg ccggagcggt gggtctgccg cagttcatgc
     5641 cgtcgagcta ccgccgctac gcggtggact tcgaccgcga cggcgccatc gatctggtcg
     5701 gcagtccgtc cgatgcgatc ggcagcatcg cgagttacat gaaggcgttc ggttgggtga
     5761 cgggcgaacc gccgacggcg ccggtgcgcc tcgcaccggg cagcgaaacg gatctcgtca
     5821 gcggattgca gcgagttcac gaccagctgg aggtgcaggg caagggcgtg gtgttctcgg
     5881 gccgcgaccc gccggccggg ctcgtttcga tcttcgaact acccacaccg ggcaagcctt
     5941 cgaagttcgt ggcgggcttc accaacttcg aagtcgtcac gcgctacaac cgctcgacgt
     6001 tttatgcgtc ggcggtgctc gagctcgcgg acgccatcca gaaagcgcac gaccgaatgc
     6061 atatggcgtc ggcggatcga taacgcagcg gcccaggtgt gggccatgac ttgccagtcg
     6121 atatgggctg agcgaatact cggccatgaa aaaccccagt cgtgttgctg ccgttctcgt
     6181 cgtgcaattc ttgttagccg ccgtggcgcc gctccaggcc aaggatgccg gctcgaaaat
     6241 tcccgccgac ctcgcggcgc gcgtgcatgc ctacgatgag gcgcaggtga agggcgacaa
     6301 ggccgccctc gaagatctcg tcgccgacga ctacgtgctg atgaacagtc gcggtcagcg
     6361 gcagaccaag accgacctga tccgcgacta cacgaaaccg ggattcaaac tcgagccgtt
     6421 cacgatcgag gagccagtcg agctcgtctg gagcgatggt gcggtgatgg gcggcgtggc
     6481 agaccttcgc ggtgtagacg acggccaggc gttcacactg cgtctgcgct tcagcgacat
     6541 ctgggccaag cgcggcggca agtggcgcgt catctacacg cacgtcaatc gcgaaactgt
     6601 aagcgcacag taatctcagc gaaacgagca agaccgagca ttcctgcgcg ccgcttgctt
     6661 tcgacgtgcc atcccctggc gcgtcggcgc gcgcaggagt acatcaatgc tttccacccg
     6721 ccatggtttg cgcaccatgt tcgcgtcgac tctttgtgcc gccgttgtca tgcaatccgc
     6781 cggcgccggc gaactgctcg accgcgcggt tctgcccgcc aacacgttct ccaccggacc
     6841 cacgtccggc cagttcgcat cgggcgccaa cggtaacatc ctgcctctca tcaacaagca
     6901 gccggtccag ggtttttccg ccgtgctgcg cggcccggtc aagggcacgt acctcgtcat
     6961 gtccgacaac ggcttcggca acaaggcgaa ctcgcccgat gcgctgctgc gcgtgaacgc
     7021 ggtgaagccg gatttcgaaa ccggcgacgt gaagccggtg aatcgcttta cgggcgacga
     7081 gctaaacgac ttcacgcccg acagcttcat gacgctgcgt gatccctgga agaaagtgcc
     7141 ctggcccatc gtggccgatg gcgtcaacta cccgggcacg cccacgggcg gcggcgcggc
     7201 gattcccgtc gatgcgtcga tcaaggctgg tcgcctgctc acgggcgcgg acttcgacat
     7261 cgaagcgttc cgccgcgcgc cggacggcac gttctggttc ggcgacgagt tcggcccttt
     7321 cctcatccac acggattcgt tcggccgcgt gctggaagcg ccgattccgc tgcccaactt
     7381 ccgcggcctg ccgagcgctg cgggtggatc agaggtgaat ccgctggtgc aggcagtcag
     7441 taatccgatt cgcacgctgg ccgcgaacct gccggactcc ggcggcttcg aaggcatggc
     7501 gctcaacaag agcggcacgc gtctctacgc gctgctcgag aaggccatca acggcgatcc
     7561 ggtgcgcacg cggctcatca tcagcgagtt ctcgctggga cagcgcaagt acacgggcaa
     7621 gacgttcgcc tatccactcg atgcgccgtc gaacgccatc ggcgacttca cggcgctgac
     7681 cgatcgtgag tacatcatca tcgagcgtga cggcgggcag ggcgatgctt ccgacccgcg
     7741 cttcaccaac cccgcgcgct tcaagaagat cttccgcatc gacctcgatg aagtggatgc
     7801 gagcggcacg ctcatcaagg aagaagtggt cgatctcatg aatatctacg atccgcgcga
     7861 cgtggccgaa gacggcggca cgaagaccgt gttcacgttc ccgttcacca ccatcgaaga
     7921 cgtgctggtg ctcgacaaca accgtctgct ggtcatcgac gacaacaact acccgggttc
     7981 ggccgggcgc gagttcggcg tgtcggataa caacgagttc atcgttgtgc atgtggcgcc
     8041 gttgctcgat tgcgaggaag acgaaggcca cggtcacggc aaacatggga agtctcatcg
     8101 caaacatcac gacaagagtg agtgcgagct ggcgcgcgac tgaagtttgc accggccacg
     8161 atttctcggt aggaaaggac tcctgccgag actcgtgacc accgttgaag ccgccgaccc
     8221 tcgaccaact gcgccgctat gcgattgccc gctcgctctt caagccgacg aagctttcga
     8281 aggcgatcga caagcttggc ttcgtgcagg ccgatcccat ccgcgcccct gcacgcgcgc
     8341 aggacctcat gctgcgccac cgggtcaccg gctatcgcgt gggcgacctc gaacgccgtt
     8401 acccgcgcct gccgatcgag gaagacttct tcgtcaacta cggattcatg ccgcgtgaac
     8461 t
//
LOCUS       DATDEU010000017         5017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_732075, whole genome shotgun sequence.
ACCESSION   DATDEU010000017 DATDEU010000000
VERSION     DATDEU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732075"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VIV63_00320"
     CDS             complement(<1..1090)
                     /locus_tag="VIV63_00320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6123065.1"
                     /translation="MSLIRTLSGLIALTAILAANLLMCALSLALFVLRGGRPKAPPQS
                     ISGAIKLLLVAPFSPGNESGGSKAVLDLANTLKPHFDLATHVIAKDAPRGGMGRRLAR
                     ALLRPLPIPDHCRDLLFGDASLSAAIAPVHTVLFEFQLTAVYLCFGQTHGKRVVVRDH
                     EVLVRKLDMERAAATGLDSLAWWLRTMSCWLVTLAAYSRANLIIVLTDADRAAIARWF
                     PFFAARTVSVPAPFEPPSLASTPPRAGPRELLMVANFFHSPNRDGLRWFLRECAPRLE
                     PGFTLHLCGIDGALSAEDLTSPHLAIVRHGFVARDEDVAALADIAVAPIISGGGVRIK
                     NLFLGSLGKALATTPLGNEGIKLVDGVHA"
     gene            1282..2337
                     /locus_tag="VIV63_00325"
     CDS             1282..2337
                     /locus_tag="VIV63_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HEY6123066.1"
                     /translation="MTDSLSRAPLQIEVCGPAAAGEWNAYVAAHPEASFYHRFEWQAL
                     NREQLGNESHFLVARRDGRIAGILPLSLVASPWFGRILCSMPYVNYGGPCADDAETED
                     ALTRRAVELTSKIGAKHLEMRARRPSGVELPVSLRKVSLTLTLNPDPEVLFKGFTSKH
                     RTNIRRAEKNGLTVRSGGLELLDTFYSVMERSWQHLGTPLYRKKYFAAILEAFPADTR
                     IFLCEKGTEPIAVAFNGYGNGVVEGMWAGGGPRARELSANYVLYWEMMREACLRGCAR
                     YHLGRSTADSGAEDFKKKWNAESQQLYWYYHRPDGGPMPELNVANPKFKLAIAAWQKL
                     PLWVTRQLGPVLARSIP"
     gene            2351..3559
                     /locus_tag="VIV63_00330"
     CDS             2351..3559
                     /locus_tag="VIV63_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY6123067.1"
                     /translation="MSFVAPAGTPLRLGEIASGLWQGSRSDTDLNALHAELARLSQRD
                     VCRSFSSGRAAMVVALRAMKAVAGSRRTRVVIPGYTCYSVAAALENTGLTPVLCDVDP
                     RTLSLDLDALARIDLSGVLAIITANLYGIPNDTAEIARIAAAGGAYLLDDAAQSLGAS
                     LSGTPAGGFGDVGLFSFDKGKNIPTMQGGALVARRGVLSEEIERQWTALPFPAPAETC
                     MNAGKLAAYSLLLKPAYFGFVRSLPGLKLGQTPYELTYPITRYSPALAGIAVRLCRRI
                     DEINGQRIANAERLRAALADVPGVELISPPAGAKPVYLRLPVLVRSAALRDRLVTALE
                     TAGIGATASYPSALADVPNVRARLAPDSGDTPAARMVAERILTLPTHAYSPPDLATRV
                     RAIVMAQESA"
     gene            3556..4917
                     /locus_tag="VIV63_00335"
     CDS             3556..4917
                     /locus_tag="VIV63_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY6123068.1"
                     /translation="MNVPAEQVAVAPPMRRRPEPKPKTTPDWMFLLFMVAFVLEYSGL
                     PNEYPVLRQMRLTTIISYSLLLLTLGKVGFGDLFSHRQTKFLMTFLVFTILSMLWAVV
                     KLKAYQSIRPFVDYLIFFYLAASLVDRPRRYMVFSVVMAGLAFWLVSQNLNRLGQEVR
                     AGSYAAPYFMGDGNDFAWGLAIMMPFCLYLFLTSKSFIIRMVGLAGLLTTLFGIMGTS
                     SRGGSLGVGAGVLFYWWFMAKRKAVGVGIIVVGLIGVLAFAPASYLNRMGSLSNTSED
                     TSAQARITTWKASMKMAVDYPLGVGAGNFSSAYGRFYLGGGNLGYAQNKWFSAHSIYF
                     RTLGEYGYLGVALLIYLIIATVVDNMKVRARILAQGDAATVPINWGNLVAMATISYAI
                     CGIFLGGLSYPHLFLLCGLTVSNKRLVGLNDAKVVKSRVRRRGAIAPTPATATHTVHE
                     RKA"
BASE COUNT          825 a         1680 c         1646 g          866 t
ORIGIN      
        1 tggcgtggac gccatcgacc agtttgatgc cctcgttgcc gagcggggtg gtcgccagcg
       61 ccttgccgag cgagcccagg aacaggtttt tgatgcgcac gccgcccccc gaaatgatgg
      121 gagcgacggc gatatcggcc agcgccgcga cgtcctcgtc gcgggccacg aacccgtgcc
      181 ggacgatggc aaggtgcggg gaggtgaggt cttccgcgct cagggcgccg tcgatgccac
      241 acaggtgcaa cgtgaatccc ggctcgagcc gcggcgcgca ttcgcgcaag aaccagcgca
      301 gtccgtcgcg attcgggctg tgaaagaagt tggccaccat caggagctca cgcggaccgg
      361 cgcgcggcgg cgtggaagcc aggcttggcg gctcaaaggg cgcgggaacg ctcaccgtgc
      421 gtgccgcgaa gaacgggaac cagcgcgcga tggccgcgcg gtccgcgtcg gtgagcacga
      481 tgatgagatt cgcgcgcgaa taggccgcga gcgtcaccag ccagcagctc atcgtgcgca
      541 gccaccaggc gagcgagtcg aggccggtgg ccgccgcgcg ctccatgtcg agtttcctca
      601 ccagcacttc gtggtcgcgc accacgacgc gtttgccgtg ggtttgcccg aaacatagat
      661 agacggcggt gagctggaat tcgaagagca cggtgtgcac gggcgcgatc gccgcggaca
      721 atgacgcgtc gccgaacagg aggtcgcggc agtggtcggg aatgggcagc ggccgcagca
      781 gcgcgcgcgc caggcggcgg cccataccgc cgcgcggagc atccttggct atgacgtggg
      841 tcgcgagatc gaaatggggt ttgagcgtgt tcgcgaggtc gagcacggcc ttcgacccgc
      901 cgctttcgtt gccgggcgaa aacggggcaa ccagaagcaa cttaatcgcg cctgaaatcg
      961 actggggcgg cgctttcggg cgcccacccc gcaggacgaa cagagccagc gacagggcgc
     1021 acatcagcag attggcggca aggatcgcgg tcagtgcgat cagcccggaa agggttctta
     1081 tgaggctcaa aacggctccc tggaagcggc cgcatcatac aacggggtgc cgccatgaca
     1141 gcccggtgac cccggaagtg aagtgtgagt tacctctcgc cttgggcgac ggtctgcgga
     1201 ggctggcccg aggggccgaa cccgccaagg taaaatcgcc gcccttcacg ctgtcgacga
     1261 agccttcccc gaatgcatgc aatgaccgat tccctgagcc gcgccccctt gcagatcgaa
     1321 gtctgtggtc ccgcggcggc cggcgagtgg aacgcgtatg tcgccgcgca ccccgaggcc
     1381 agtttttatc accgcttcga atggcaggcg ctcaaccgcg agcaactcgg caacgaatcg
     1441 catttcctcg tcgcgcgccg tgacggccgc atcgccggca tcctgccgct gtcgctggtc
     1501 gccagcccct ggtttggccg catcctctgc tcgatgccgt acgtcaacta cggcggcccc
     1561 tgtgccgacg atgccgaaac cgaagacgcg ctgacgcgcc gcgcggtcga gctcacctcg
     1621 aaaatcggcg ccaagcatct cgagatgcgc gcgcgccgcc cgagtggcgt cgaactaccg
     1681 gtgtcgctgc gcaaggtcag cctcacgctg acgttgaatc cggatcccga agtgctgttc
     1741 aagggtttca cgtcgaagca ccgtaccaac atccgccgcg cggagaagaa tggactgacg
     1801 gtgcgttccg gcggcctcga actgctcgac accttctact ccgtcatgga gcgcagctgg
     1861 cagcacctgg gcacgccgct ttatcgcaag aagtatttcg ccgccatcct cgaggccttc
     1921 ccggccgaca cccgcatttt cctttgcgag aagggcacgg agccgatcgc cgtggctttc
     1981 aacggatacg gcaacggcgt ggtcgaaggc atgtgggccg gcggcgggcc gcgcgcgcgc
     2041 gaactctcgg ctaactacgt gctgtattgg gaaatgatgc gcgaggcctg cctgcgcggt
     2101 tgcgctcgct atcacctcgg ccgttcgacc gccgattcgg gcgccgaaga tttcaagaag
     2161 aagtggaatg ccgaatcgca gcagctctac tggtactacc accgccccga cggcgggccg
     2221 atgccggagc tgaacgtggc gaaccccaag ttcaagctgg cgattgccgc ctggcagaag
     2281 ctgccgctgt gggtcacgcg ccaattgggc ccggtgctcg cgcggtcgat tccgtgatga
     2341 gcgtgacccg atgagcttcg tcgcacccgc cggcacgcca ctgcggctcg gtgaaatcgc
     2401 ctcggggctc tggcagggct cgcgttccga tacggacctc aacgcgttgc atgccgaact
     2461 ggcgcgcctg tcgcaacgcg acgtgtgccg gtcgttctcc tcgggccgcg ccgccatggt
     2521 ggtggcgtta cgggcgatga aagcggtggc cggcagccgg cgcacccgcg tggtgatccc
     2581 gggctacacc tgttactcgg tggccgcggc gctcgagaac acgggactga cgcccgtgct
     2641 ctgcgacgtg gacccgcgca cgctgtcact cgacctcgac gcgctggcgc gcatcgatct
     2701 gtccggtgtg ctggccatca tcaccgccaa cctgtacggc atcccgaacg acacggcgga
     2761 aatcgcgcgt atcgcggccg ccggcggtgc ctatctactg gacgacgccg cgcagtcgct
     2821 gggcgcgagc ctttccggca cgcccgcggg tggcttcggc gacgtgggcc tcttcagctt
     2881 cgacaagggc aagaacattc cgaccatgca gggcggggcg ctcgttgccc gccgaggcgt
     2941 gctgagcgag gagatcgagc ggcagtggac cgcgcttccg tttcccgcgc ccgccgagac
     3001 ctgcatgaat gccggcaaac tcgcggctta ttcgctgctg ctgaaacctg cgtacttcgg
     3061 tttcgtacgg tcgctgcccg gtctcaagct cggccagacg ccgtatgagt tgacctatcc
     3121 gatcacgcgc tacagcccgg cgctcgccgg gatcgcggtg cggctgtgcc ggcgcatcga
     3181 cgagatcaac gggcaacgca tcgcgaacgc cgagcgcctg cgcgctgcgc tcgcggacgt
     3241 gccgggcgtc gaactcatat cgccgccggc tggtgcgaag cccgtgtatc tgcggctgcc
     3301 ggtgctcgtg cggtcggccg cgctgcgtga ccgcctcgtg acggcgctcg agacggccgg
     3361 cattggcgcc acggcgtcgt accccagcgc gctcgcggac gtgcccaatg tgcgcgcgcg
     3421 cctcgcgccc gactcgggcg acacgcctgc agcgcgcatg gtcgccgagc gcatactcac
     3481 gttgcccacg cacgcgtaca gcccgccgga cctggcaacg cgcgtgcgcg cgatcgttat
     3541 ggcgcaggag agcgcatgaa cgtgcccgct gaacaggttg ccgtcgcgcc gccgatgcgc
     3601 cgccggcccg aacccaagcc gaagacgacg ccggactgga tgttcctgct gttcatggtg
     3661 gcgttcgttc tcgagtactc cggcttgccg aacgagtacc cggtgcttcg ccagatgcga
     3721 ctcaccacca tcatcagtta cagcctgctg ttgctgacgc tcggcaaggt gggcttcggc
     3781 gatctgttct cgcatcgcca gaccaagttc ctcatgacct tcctggtgtt caccatcctg
     3841 tcgatgttgt gggcggtggt gaagctcaag gcctatcaat ccatccggcc tttcgtcgac
     3901 tacctgatct tcttctacct ggcggcgagc ctcgtcgacc gcccgcggcg ctacatggtg
     3961 ttcagcgtcg tcatggcggg cctggcgttc tggctggtct cgcagaatct caatcggctc
     4021 gggcaggaag tgcgcgcggg ctcgtacgcg gcgccgtact tcatgggcga tggcaacgac
     4081 tttgcctggg gcctcgccat catgatgccc ttctgcctct acctgttcct gacctcgaag
     4141 agcttcatca tccgcatggt gggcctcgcg ggacttctga ctaccctgtt cggcatcatg
     4201 ggcacgtcgt cacgcggcgg atcgctcggc gtcggcgcgg gcgtcttgtt ctactggtgg
     4261 ttcatggcca agcggaaagc ggttggcgtg ggaatcatcg tggtcggcct gatcggcgtg
     4321 ctggcgttcg cgcccgcgag ttatctcaac cgcatgggtt cgctgtcgaa caccagcgaa
     4381 gacacgtccg cacaggcgcg catcacgacg tggaaggcgt ccatgaagat ggccgtggac
     4441 tatccactcg gcgtcggcgc cggcaacttc tcgtccgcgt acggtcgttt ctatctcggc
     4501 ggcggcaacc tcggttacgc gcagaacaag tggttctcgg cgcacagcat ctatttccgc
     4561 acgctcggcg agtacggcta tctcggcgtc gcgctgctga tctacctgat catcgccacg
     4621 gtcgtcgaca acatgaaggt acgtgcgcgc atcctcgcgc agggcgacgc ggcgaccgtc
     4681 cccatcaact ggggcaacct cgtggccatg gcgacgatct cctacgccat ctgcggcatc
     4741 ttcctcggcg gcctgagtta tccgcacctg ttcctgctct gcggcctcac ggtgtccaac
     4801 aagcggctcg tgggtctcaa cgacgccaag gtcgtcaagt cgcgcgtccg acgccgcggc
     4861 gcaatcgccc ccacgccagc caccgccact cacaccgtgc atgaacgcaa agcctgaaat
     4921 cctgataccg gatactccga cgcccgcgac gcagctcgcg cggcgcattc gcgtggtaca
     4981 gctgacgggc gcgctggact tcggtggtct cgagaac
//
LOCUS       DATDEU010000018         5607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1899533, whole genome shotgun sequence.
ACCESSION   DATDEU010000018 DATDEU010000000
VERSION     DATDEU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899533"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..936)
                     /locus_tag="VIV63_00340"
     CDS             complement(34..936)
                     /locus_tag="VIV63_00340"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY6123069.1"
                     /translation="MLPAKTASPPVHTTSLRGLKKIHTGKVRDIYEIDAHSMLIVTTD
                     RLSAFDVVLPDPIPDKGRVLNSISNFWFERTGHIVPNHLTGRNIAEVVSDPAERALLA
                     DRAVIVKRLKTVPLEAVVRGYVIGSGWKDYQRTGAISGIKLPAGLRQAAKLPQTLLTP
                     STKAAIGDKDETISFEQASKIVGADTAALVRDTAIALYEFASKHARERGIIIADTKFE
                     FGIDEAGRLTLIDEVLTPDSSRFWPMDTWQPGTSPPSFDKQFVRDYLETLDWNKRAPG
                     PRLPPAIIEKTGAKYAEALRRLTS"
     gene            967..1803
                     /gene="djlA"
                     /locus_tag="VIV63_00345"
     CDS             967..1803
                     /gene="djlA"
                     /locus_tag="VIV63_00345"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HEY6123070.1"
                     /translation="MSIIGKVVGVIVGLLIFRSPWGAIIGLILGHFYDQSVVTVRRTG
                     GGGGAGVLEIGERFFTATFEVMGHVAKSDGRVSEAEIAAARKVMAELRLDGAQIHAAI
                     AHFTRGKGSDFDLPTTMQEFAEACANRPDLVRVFLEVQVRAALEGVDMQGPARLAIQK
                     VADLLEVSRIELAHMEAVLRLRREQFKAGPRNGNGNGGSAGSSQARSGMQVGAAYQIL
                     EVDPKATDEEVAKAYRRQLSKHHPDKLKANGLPESMLEHAKQRTQQIIEAYELIKSTR
                     GA"
     gene            1815..2489
                     /gene="rpe"
                     /locus_tag="VIV63_00350"
     CDS             1815..2489
                     /gene="rpe"
                     /locus_tag="VIV63_00350"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY6123071.1"
                     /translation="MPKHTPWIAPSILSADFARLGDEVNAVLAAGADVVHFDVMDNHY
                     VPNLTIGPLVCEALRKHGVTAPIDVHLMVKPVDRIVPDFAKAGATYISFHPEASEHVD
                     RTIELIREHGCKPGLVLNPATPLTCLDHTLKKLDLVLLMSVNPGFGGQKFIPAVLDKV
                     RAVRKLIDASGRDIRLEIDGGVKVDNIGEIARAGADMFVAGSAIFGAKDYQATISAMR
                     AEIASA"
     gene            complement(2499..3416)
                     /locus_tag="VIV63_00355"
     CDS             complement(2499..3416)
                     /locus_tag="VIV63_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6123072.1"
                     /translation="MRKVALLLSLSLSASVLAQQPAATAPASLVREGVTEKLNQHVWA
                     IPDGGASLVPNVGIVVGKKAVLVVDTGMGTRNGETVLREVAKVAAGKPIYIVTTHVHP
                     EHDMGAHMFPKDSKLIRSKDQIEDIAAGAGTNLVPVFAQRSALNVELLEGAKHRDADI
                     VFDKDHTLDLGGLEAKIYAMGTNHTHGDTVILVDGVLFSGDVAMRPQPSFANPTAKIS
                     HWLESLDRLEALKARQLVPSHGAFGDNAIIAGYRSYLTRIRDRSAELKKSGKSQDEVV
                     QIITDEMSAQYPDKNRLAGAIRAGFNEAP"
     gene            complement(3421..3960)
                     /locus_tag="VIV63_00360"
     CDS             complement(3421..3960)
                     /locus_tag="VIV63_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY6123073.1"
                     /translation="MRFILLALLIAAPGAGAFAATPSPDQTARNLEVLKRYGEVGRQR
                     DYAQQAAMWAPNALNNGRPVTPDDIRSQLEDLHRTFPDLRSEIVETVSSGDFVIVLQR
                     TSGTHRGIAQTEIYGGMLRGVRPTGRRFEILRSHWWRLTDGKIVWHQVVADDLGMMRQ
                     LGLIADTLPADKLVAPTKE"
     gene            complement(4169..4804)
                     /gene="crp"
                     /locus_tag="VIV63_00365"
                     /pseudo
     CDS             complement(4169..4804)
                     /gene="crp"
                     /locus_tag="VIV63_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016506431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
     gene            complement(4941..5594)
                     /gene="coq7"
                     /locus_tag="VIV63_00370"
     CDS             complement(4941..5594)
                     /gene="coq7"
                     /locus_tag="VIV63_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017476331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEY6123074.1"
                     /translation="MAPQLDSLLSAADRALRALLAPPASGRPVPPAPPGTGRDIQKSD
                     AALSPDARRESAALMRVNHAGEMAAQALYHAQALFARDPEVREFMLHAAREETDHLAW
                     CETRLKELGGRPSVLNPLWYAGSFGIGALAALLGDRASLGFVAETERQVEGHLKSHLE
                     QLPAEDLRSRAIVEAMCHDEVGHGQQAQSAGAASLPGPVRELMRRTAQIMTHTSYRL"
BASE COUNT          969 a         1787 c         1805 g         1046 t
ORIGIN      
        1 acagcgtgtc cttcgagggc ggcgaaacga cgatcagctc gtgagtcggc gcagcgcttc
       61 ggcgtacttg gcgccggtct tttcgatgat cgcaggcggc aatcgtggac ccggcgcgcg
      121 cttgttccag tcgagcgtct cgagatagtc ccgcacgaac tgcttatcga agctgggagg
      181 gctcgtgccc ggttgccacg tgtccatcgg ccagaagcgc gaagaatccg gggtgaggac
      241 ctcgtcgatg agggtcagcc ggcccgcttc gtcgattccg aactcgaact tcgtatcggc
      301 aatgatgatg ccgcgctcgc gcgcatgctt cgatgcgaat tcgtagagcg cgatcgctgt
      361 gtcgcgcacg agcgccgcgg tgtcggcgcc cacgatcttc gaggcctgct cgaagctgat
      421 ggtctcgtcc ttgtcgccga tcgccgcctt ggtggacggc gtgagcagcg tctgcggcag
      481 tttcgcggcc tgccgcaaac cggccggtag tttgatgccg gaaatcgcgc cggtcctctg
      541 gtagtccttc cagcccgagc cgatcacgta gccgcgcacc acggcttcga gtggcacggt
      601 cttgagccgc ttcacgatca cggcccgatc ggcgagcagc gcgcgctcgg ccggatcgct
      661 gaccacttcg gcgatgttcc ggccggtcag atggttcggg acgatatgcc cggtccgttc
      721 gaaccagaag ttcgagatcg aattgagcac gcggcccttg tcgggaatcg gatccggcag
      781 caccacgtcg aaggccgaga gccgatcggt ggtcactatg agcatggagt gcgcgtcgat
      841 ttcgtagatg tcgcgcacct tgccggtgtg gatcttcttc agcccgcgaa gagaggtcgt
      901 atggacgggc gggctggctg ttttggctgg tagcatagtt ggcatgcatc ttaacaagtg
      961 cccccgatga gcatcatcgg aaaagtcgtc ggcgtgattg ttggtctgct gatcttccgg
     1021 agcccctggg gcgccatcat cggcctcatc cttggccact tctatgacca gagcgtcgtg
     1081 accgtgcgcc gcacgggcgg cggcggtggc gcaggcgttc tcgaaatcgg cgagcgcttc
     1141 ttcaccgcca cgttcgaggt catggggcac gttgccaagt cggacggccg cgtctcggag
     1201 gcggagatcg ccgcggcgcg caaggtcatg gcggagctgc ggctcgacgg cgcgcaaatt
     1261 cacgccgcga tcgcgcactt cacgcgcggc aagggcagcg atttcgacct gccaaccacc
     1321 atgcaggaat tcgccgaggc ctgcgcgaac cggccggatc tcgtgcgcgt attcctcgaa
     1381 gtacaggtgc gcgcggcgct ggaaggcgtg gacatgcagg gtcccgcgcg cctcgcgatt
     1441 cagaaagtcg ccgatctcct ggaagtgtcg cgcatcgagc tcgcgcacat ggaagcggtg
     1501 ttgcgactgc gccgcgaaca gttcaaggcg ggcccgcgca atggcaacgg caacggcggt
     1561 tcagccggct cctcgcaggc gcgcagcggc atgcaggtcg gagcggccta ccagattctc
     1621 gaagtggacc cgaaggcgac ggacgaggaa gtcgcgaaag cgtaccggcg ccagctctcc
     1681 aaacatcatc cggacaagct caaggccaat ggcctgcccg aatcgatgct cgaacacgcc
     1741 aaacaacgta cgcagcagat catcgaggcc tacgaactca tcaagtccac gcggggcgcg
     1801 taaactccat gtccatgccc aagcacacgc cctggatcgc gccatccatc ctctccgccg
     1861 acttcgcaag gctcggagac gaagtgaacg cggtccttgc agcgggcgcc gacgtcgtgc
     1921 acttcgatgt catggacaat cattacgtcc ccaatctcac catcggcccg ctggtgtgtg
     1981 aggcgctgcg caagcacggc gtcaccgcgc ccatcgatgt gcatctcatg gtgaagcccg
     2041 tcgatcgcat cgtgccggac ttcgcgaaag cgggcgccac ctacatttcg ttccatcccg
     2101 aagccagcga acacgtcgac cgcacgatcg agctgatccg cgaacacggc tgcaagccgg
     2161 gactcgtgct caatcccgcg acgccgctga cctgtctcga tcacacgctg aagaagctcg
     2221 atctcgtgct gctcatgtcg gtgaatccgg gtttcggcgg gcagaagttc attcctgccg
     2281 tcctcgacaa ggtgcgcgcc gtgcgcaaac tcatcgacgc ttccggccgc gacatccgcc
     2341 tggagatcga cggcggcgtg aaggtcgaca acatcggcga gatcgcgcgc gccggcgccg
     2401 acatgttcgt cgcgggttcg gcgatcttcg gcgcgaagga ttaccaggcg accatctccg
     2461 ccatgcgcgc ggagattgcc tcggcgtaaa ccgtcgcgct acggcgcctc gttgaaccct
     2521 gcgcggatcg cgcccgcgag ccggttcttg tccgggtact gcgcggacat ttcatcagtg
     2581 atgatttgca ccacttcatc ctgcgacttg ccggatttct tgagctcggc gctgcgatcg
     2641 cggatgcgcg tgagatagct gcgataaccg gcgatgatgg cgttgtcgcc gaaggcgccg
     2701 tggctcggca ccagttgtct ggccttgagc gcctcgagcc ggtcgaggct ttccagccag
     2761 tgactgatct tcgcggtcgg gttcgcgaac gacggttgcg ggcgcatggc tacgtccccc
     2821 gagaacaaca cgccatcgac caggattacg gtgtcgccat gcgtatggtt ggtgcccatc
     2881 gcgtagatct tcgcctcgag tccgcccagg tccagcgtgt ggtccttgtc gaagacgatg
     2941 tcggcgtccc gatgtttcgc gccctcgagc agctcgacgt tgagcgccga acgctgcgcg
     3001 aataccggga cgaggttcgt gcccgcgccc gcggcgatgt cctcgatctg gtccttcgag
     3061 cggatcagtt tcgaatcctt cgggaacatg tgcgcgccca tgtcgtgttc ggggtgcacg
     3121 tgggtcgtca cgatgtagat aggcttgccc gcggccactt tcgcgacttc gcgaagcacg
     3181 gtttcgccgt tgcgcgttcc catgcccgtg tcgaccacca gcacggcctt cttgccgacg
     3241 acgatgccga cgttgggcac gagcgaggcg ccgccgtcgg gtatggccca gacgtgttgg
     3301 ttaagctttt cggtgacgcc ttcgcgcacc agggacgctg gcgcggtcgc cgcgggctgc
     3361 tgggcaagga cgctcgccga gagggacagg gacaacagca acgctacctt gcgcatacgt
     3421 tcattccttg gtgggcgcga ccagtttgtc ggcgggcaaa gtatcggcga tcagaccgag
     3481 ctgccgcatc atcccaaggt cgtccgccac cacttggtgc catacgatct tgccgtccgt
     3541 cagccgccac cagtgcgacc gcaggatttc aaagcgccgg ccggtcggtc gcacaccgcg
     3601 cagcatgccg ccgtagattt ccgtctgcgc gatgccacgg tgcgtaccgg aagtgcgctg
     3661 gagcacgatg acgaagtcgc ccgaactcac ggtctcgacg atttccgagc gcaggtcggg
     3721 aaacgtgcga tgcaggtcct cgagttgtga gcggatgtcg tctggcgtca ccggccggcc
     3781 gttgttgagc gcgttcggcg cccacatcgc cgcctgctgc gcgtagtcgc gctggcggcc
     3841 cacctcgccg tagcgcttga ggacttcgag gttgcgcgcg gtctggtcgg gtgagggcgt
     3901 ggccgcaaac gcacccgcac ccggtgccgc gatcagaagg gccagcagga tgaatcgcat
     3961 agaagtttgc tccgcatagc agaacgccgg ctgatgccgg cgttcaatcg aggtgcgtcc
     4021 ccttttgcta gcaaatgggg agtgtcccca tttactcgtc gtcgtcctcg tcttcgtcgt
     4081 catcatcttc gacacgcgtg ttgcgtaccg agccgccacc cttgccgccc tggacgggaa
     4141 cgacaaccgg gcgcgtcttc atcttcggac gagcgttgaa gacgacgatg gtcttgccgg
     4201 tggcgcgcaa gagaccctgg tcttccagga ctttgagcac gcggcccgcc atttcacgcg
     4261 agcaacccac gagacgtgag agctcctggc gagacacctt gatctgcatg ccctcgggat
     4321 gggtcatggc atccggttcg ccgcacaggt ccatgatggc gtgcgccaca cggccggtga
     4381 catcgacgaa cgcgagatcg gacagcttgc ggttggtccg gtcgagccgc gtggcgagct
     4441 gcgtcgccag ctcgaacacc aggccgggac tttccgccgc gatctggcgg aagcgcggat
     4501 aggtcatttc cgcgagttcg caatgattgc gcgtgcgcac ccaggcgctg cgcaatggcg
     4561 actcgtggaa caagcccatc tcgccgaaga actgcccctt gttgaggtag gcaaggacca
     4621 tctcgttgcc ttcctcgtct tccaccatca cttcaaccga gccgctgatg atgtagtaga
     4681 ggacatcggg cagatcgccc gcgtggatca caaccgtttt tgacggaact gtgcgaatgc
     4741 ggcagtagct caaaaagcgg ttgattgccg gtatgtcgct gaggggtttg accgcctctt
     4801 ccatctgact ctcccttgct gcttgcattt atcgccccct gccatcgcat taagtcttat
     4861 tacataactt aatgccaagt caggcaaaag aacacggatc aacagtgacc cgcgtcacag
     4921 gcgccccatc taaagcccat tcataaacga tacgaagtat gcgtcatgat ctgggctgtt
     4981 cgccgcatca attcgcgtac cgggcccggc aacgacgcgg cgccggcact ttgcgcctgc
     5041 tggccatgcc cgacctcgtc atggcacatg gcctcgacga tagcccggct gcgcaggtct
     5101 tcggccggaa gttgctcaag gtgactcttc agatggccct cgacctggcg ctcggtttcg
     5161 gccacgaacc ccaggctggc gcgatcgccg agcagtgcgg ccagcgcgcc aatgccaaag
     5221 gatcccgcat accacaaggg gttgagcacg ctggggcggc cacccagctc cttgagccgg
     5281 gtctcgcacc aggcgagatg gtcggtttcc tcgcgggccg catggagcat gaattcccgg
     5341 acttccgggt cacgcgcgaa cagtgcctgg gcgtggtaca gcgcctgggc ggccatctcc
     5401 ccggcgtggt tcacgcgcat gagggccgcg gactcccggc gggcgtcggg tgaaagggcc
     5461 gcatccgact tctggatatc ccgcccggtg cccggggggg cggggggtac cggccggccg
     5521 gaagccgggg gcgcgagcaa agcccgcagc gcgcggtccg ctgctgataa caaggagtcc
     5581 agttggggcg ccatggagcg gacgtat
//
LOCUS       DATDEU010000019         3425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_732528, whole genome shotgun sequence.
ACCESSION   DATDEU010000019 DATDEU010000000
VERSION     DATDEU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732528"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..2454)
                     /locus_tag="VIV63_00375"
     CDS             complement(160..2454)
                     /locus_tag="VIV63_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY6123075.1"
                     /translation="MRATFRRDVIIPRFGAALAALSLVLGIAFAAPAHAEKLALERLF
                     AAPDLAGSNLRGVEISPDGGLVAYLRARDDDKDRFDLWAFDVRQSRHRKLVDSRTLAG
                     ADRALSAEEEARRERQRTSAYSGIVEYSFAPDSRQLLIPLNGDLYVYDLGRKPADAVR
                     RLTHTEAFETDARFSPRSHYVSFVRDQNLYVIDLATGVERAVTREGGGTVSFGMAEFI
                     AQEEMDRDTGYWWAPDERHIALARVDESPVAEVDRIEIGATGVTTVRQRYPATGARNA
                     LVSLLVADLAADSRLPLDLGGETDIYLPRVDWFPDSRGIAVQRQSRDQKTLELMRFDA
                     LSGRGRVLLTERSEHWVPLHRELTFLRNSPQFIWASSRDGFQHLYLYGNDGTLVRQLT
                     SGEYMVVGESPEAAIRAVDERARRVYFIANRPSPLERQLFWVSLDAPGEPQRVSAEAG
                     WHSVAMSQNARVWVDTFSDANTPRSATLRSIDGKALATLLANRLDDKHPYARYLDEHV
                     RAEFGTIAAADGQLMHYKLLKPRNLEAGKRYPVLVDVYGGPGAQRVSNAWGSLFHQYL
                     VQHGYVVFLLDNRGSGMRGARFETALGLRMGDIEVRDQVRGVEFLRGLPFVDAKRIGM
                     YGWSYGGYMTLMCLMQAPDAFAAGVAGAPVTDWTLYDTHYTERYLSTPAGNADGYRRS
                     NVLEYANKLERPLLLVHGMADDNVLFAHSTVLMKKLQDLQKPFDLMTYPGGKHGLIRQ
                     SVSGWHAHSNIVRFFDRELGAGPR"
     gene            2500..>3425
                     /locus_tag="VIV63_00380"
     CDS             2500..>3425
                     /locus_tag="VIV63_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123076.1"
                     /translation="MVNNPRYLGALAAAAITALFGACVSPKATHAPVAEPAAFQYPAS
                     VRGDVVDDYHGVRVPDPYRGFEDATAQTTRDWVSAQNALSQPYLERLPQRAWLGGRLK
                     QLWNYDRFGVPQREGGKYFFMRNDGTQDQSVLYVADALNATPRVLVDPNGKRDDATIA
                     LSQWVPSPDGNLLAYALSDGGTDWNTWHFRRVADGTDLPVILKFSKFYSVSWARDSAG
                     VYYSRYPLKPGQKAEEAERGDDSGRPDVYFHKLVDAQTSDSLVYQVTDHPTRVPSGQV
                     TEDGRYLLIGLFDGYETNGMLVKDLRKAGAKP"
BASE COUNT          596 a         1127 c         1121 g          581 t
ORIGIN      
        1 gcggcaaaca cggcctgatt cgccaaaacg tgaccggctt gcatgcccac accaacctgg
       61 tgcacttctt cgaccgcgag ctcggtcccg ggcctcgcta gtcagcgtca cgaggaggtc
      121 cggccccttt tgctaggaaa cgaggaatgt ccccattaat cagcgtgggc cggcgccgag
      181 ctctcggtcg aagaagcgca cgatatttga atgcgcgtgc cagccggaga cgctctggcg
      241 aatcaggcca tgtttgccgc cggggtaggt catgaggtcg aaaggcttct gcaggtcctg
      301 cagcttcttc atgagcacgg tgctgtgcgc gaatagcacg ttgtcgtcgg ccatgccatg
      361 caccagcagc agcggcctct ccagcttgtt cgcatactcg agcacattgc tgcgccgata
      421 cccgtccgca ttgcccgccg gggtagatag gtatcgctcg gtgtaatggg tgtcgtacag
      481 cgtccagtcg gtgaccggcg cgccggccac gccggccgcg aacgcatccg gcgcctgcat
      541 caggcacatc agcgtcatgt atccgccgta gctccagccg tacatgccga tacgcttcgc
      601 gtcaacgaac ggcagcccgc gcaggaattc gacgccgcgc acctggtcgc gcacttcgat
      661 gtcacccatg cgaaggccga gcgcagtttc gaagcgcgcg ccgcgcattc cgctgccgcg
      721 gttgtcgagc aggaaaacca cgtagccgtg ctgcacgaga tattgatgga agaggctgcc
      781 ccacgcgttg ctgacgcgct gcgcgcccgg gccgccatag acatcgacga gcaccggata
      841 acgcttgcct gcttccaggt tgcggggctt cagcagcttg tagtgcatga gctggccgtc
      901 agccgcggcg atggtgccga actccgcccg cacgtgttca tcgagatagc gcgcgtacgg
      961 atgcttgtcg tcgaggcggt tggcgagcag cgttgccagc gcctttccgt cgatggagcg
     1021 caaggtcgcg ctgcgcggag tgttcgcgtc ggaaaaggtg tcgacccaca cgcgcgcatt
     1081 ctgcgacatg gcgacgctgt gccagcccgc ctcggcggac acacgctgcg gctcgcccgg
     1141 cgcgtccagc gacacccaga agagctggcg ctcgagcggc gaaggccggt tggcgatgaa
     1201 gtagacgcgc cgcgcgcgtt cgtcgacggc gcggatggcg gcctcgggcg attcgcccac
     1261 caccatgtat tcgcccgacg tcagctgccg gaccagggtt ccgtcgttgc catacagata
     1321 gagatgctgg aagccgtcac gcgacgacgc ccagatgaac tgcggcgaat tccgcaggaa
     1381 cgtgagctcg cgatgaagcg gcacccagtg ttcgctgcgc tcggtgagca gcacgcgccc
     1441 gcggcccgac agcgcgtcga agcgcatcag ctcgagtgtc ttctggtcgc ggctctggcg
     1501 ctgcacggcg atgccgcggc tgtccgggaa ccagtccacg cgcggtagat agatgtccgt
     1561 ttcgccgccg aggtcgagcg gaagccggct gtccgccgcg aggtcggcga cgagcaggct
     1621 cacgagcgca ttacgcgcgc cggtggcggg gtaacgctgc cgcacggtgg tgacgccggt
     1681 ggcgccgatc tcgatgcgat cgacctccgc gaccggcgat tcgtcgacgc gcgcgagcgc
     1741 gatgtgccgc tcgtccggcg cccaccagta gccggtgtcg cgatccatct cttcctgcgc
     1801 gatgaattcg gccatgccga agctcaccgt gccgccgcct tcgcgcgtca ccgcgcgttc
     1861 cacgcccgtg gcgaggtcga tgacgtagag attctggtcg cgtacgaaac tcacgtagtg
     1921 cgatcgcggc gagaaacgcg cgtcggtttc gaatgcttcg gtgtgggtga gccggcgaac
     1981 cgcatccgcg ggctttcggc cgaggtcgta cacatacaga tcgccgttga gaggaatcag
     2041 cagctggcgg ctgtcgggtg cgaagctgta ttccacgatg cccgagtacg ccgaggtgcg
     2101 ctggcgctcg cggcgcgctt cttcttcggc cgacagcgcg cgatctgcac cggccagcgt
     2161 gcgtgaatcc acgagcttgc gatggcgcga ctggcgcacg tcgaatgccc agaggtcgaa
     2221 gcgatccttg tcgtcatcgc gagccctaag gtaagccacg agcccgccat ccggcgagat
     2281 ctcgaccccg cgcaggttgc tgcccgcaag atctggtgcc gcgaacagac gttcgagcgc
     2341 gagtttctcc gcgtgagcgg gcgcggcgaa cgcgatgccg agcaccagtg acagcgctgc
     2401 gagcgctgcg ccgaagcgag gaataatcac gtcgcgccgg aaagtcgcgc gcataccgtt
     2461 tgctacactg ccgatcaatt tcatttcggg agtttaggga tggtcaacaa tcctcgctac
     2521 ctgggggcgc tcgccgcggc cgcgataacc gcactcttcg gcgcatgcgt ctctccgaaa
     2581 gccacgcacg ctccggtcgc cgagcccgcc gcgttccagt atccggcgag cgtgcgcggc
     2641 gacgtggtag acgactatca cggcgtgcgc gtgccggatc cctaccgcgg gttcgaagat
     2701 gctacggcgc agacgacgcg cgactgggtg agcgcccaga acgcactctc gcagccgtat
     2761 ctcgaaaggc tgccgcaacg cgcgtggctc ggcgggcgac tcaagcagtt gtggaactac
     2821 gatcgtttcg gtgttccgca gcgcgaaggc ggcaagtatt tcttcatgcg caacgacggc
     2881 acccaggatc agagcgtgct gtacgtcgcc gatgcgctca atgccacgcc gcgcgtgctg
     2941 gtcgacccca atggcaagcg cgacgacgcg accatcgcgt tgtcgcaatg ggtgccgagc
     3001 cccgatggca acctgctcgc ctacgcgctc tccgatggcg gcaccgactg gaacacctgg
     3061 cacttccgcc gcgtggccga cggcaccgat cttccggtca tcctcaagtt cagcaagttc
     3121 tactcggtct cgtgggcgcg cgacagcgcg ggcgtctact acagccgtta tccgctcaag
     3181 cccggacaga aggccgaaga ggccgagcgt ggcgacgact ccggccggcc cgatgtctat
     3241 ttccacaagc tcgtcgacgc acaaacctcc gacagcctgg tgtaccaggt caccgatcat
     3301 ccgacacgcg tgccttcggg ccaggtgacg gaagacggcc gctatctgct catcggtttg
     3361 ttcgacggct acgaaaccaa cggcatgctg gtgaaggacc tgcgcaaggc gggtgcgaaa
     3421 ccgca
//
LOCUS       DATDEU010000020         5935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1753891, whole genome shotgun sequence.
ACCESSION   DATDEU010000020 DATDEU010000000
VERSION     DATDEU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753891"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VIV63_00385"
     CDS             complement(<1..311)
                     /locus_tag="VIV63_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123077.1"
                     /translation="MKSIKTTALSLALSFCALAFSMASLSAETFRGNWTIAPAKEAGK
                     VRLALMHRMHGGSSHHESDWPASALQGLDLSASGKRDVQFNITRDAGRFDCEGYLKDG
                     EG"
     gene            complement(332..2113)
                     /locus_tag="VIV63_00390"
     CDS             complement(332..2113)
                     /locus_tag="VIV63_00390"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY6123078.1"
                     /translation="MNATTTLMQTLAWSLLHFLWQGAAIAAVTASLLFVMRKPATRYL
                     VGIGALVLMLVSFGVTFSLLGESTTAPADFPTAQAPAAASASTLEATANSVDELTTDE
                     QAATFRSEDFLWVSRAWLVGVFFFALRIAFGLLVLEQLRRRNLVALPPALVARFEALQ
                     HRLGIHRAIRYCECHLLSVPAVIGFFRPVVLLPMRALTGLSPEQLEAVIAHELGHIKR
                     FDVAVNFVQVIAETLFFFHPAIWWLNKRIRADREDCCDDVAIAACGGTVGYARALATM
                     EGWRDVPDFAMAATGSSVAARVARLLGVRTQEPGARTAGMVTATLVLATALVTGALTV
                     GVALPARAQGNDVSLQQLVTQAEAAVAVQANSVENVEQVEQVQQPAPKPARAPRPAVA
                     PAAAVAPRAPLAPLAPTAPLAPPKAPATAPTVSYIEEMRAAGYDNLDVDDLIALKVQD
                     VTPGYIRDIRSSGYNPNASELIAMKIHDVTPAYIKGMREAGFKPDAQEIIAMKIHDIT
                     PEYVQQIRAAGFQPDGQELIALKVHGVTADYQRKLAAAGYKMDTQQLIQAKVMDITPE
                     FIREVNAHGFKNLTVEKLIQLKNADIL"
     gene            complement(2110..2499)
                     /locus_tag="VIV63_00395"
     CDS             complement(2110..2499)
                     /locus_tag="VIV63_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY6123079.1"
                     /translation="MKTDPKLQPTPSELAILQILWSRGPSTVREIHEVLSKEKDMGYT
                     SALKFLQIMTSKGLVTRTEEQRAHIYSAHQPAETTKQQFAADVLKRVFSGSASQLMQH
                     ALSGRRSSKKEIDEMRRMLDEYERKLP"
     gene            2614..5919
                     /locus_tag="VIV63_00400"
     CDS             2614..5919
                     /locus_tag="VIV63_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEY6123080.1"
                     /translation="MSIRFCAPALGVLFLVACTSRPSTPDGAMLLDEEVTLRREAKVD
                     TAQREIEVDSGSIVVVFVDEKLTDVKVRLAIVGADGEPLTVEVENNLRGSGLEVAAIE
                     APRGSRVSITLTGPQDSAAPGHVHLNVRRFDVHPEESKFTAQVAAFKSWSSATNASLR
                     TDAVKATALAEMDRAISGLSSPPGDAALAAKARIVKANMLLFFRVDWRESRAEAQRAA
                     DAFGALPKVDTLNVSRAKLIESMALLQIAADRKATNPTSEEAVSLARGILAGLIDPLS
                     MFDPVERARAITVLGQVDYATTRLDDADKRYAEAQAIYQAAGYAAGEREIRCHLAVVL
                     LERGRFRDAALAFVALLPEVDEIASPEMRVTVLLGAARGQSFSARTDPAAELQIRALA
                     VAREYQLRDKEASALHGLGHVYANRGDPLQARAYYAEALRAARNGSDVMSYVWALASA
                     GVVARDDGDHDQAVALHKEAVSLASNPIARVRTLRELGLDYYFARDFAAAIVEFRLAL
                     DVDLHDPVHHAYSDVKRNLAQALIETDDPSPATFKEAARLLDESLKSCLRVGDKQGEI
                     GAHRITGQLLANQGRSKEALVEYERTFALADAYRGSSASPEARRATLPHVQQAFRGYL
                     DLEMKSVVARGAGKVQPASNREMAALRKLEMARDANFGAARTGELDVETARQVDALLS
                     QMANKSLAITAMLKRELAKSEEAELASLQSGMADLHAELDRLRTAAATKAAVAEKAQA
                     ITTRDWRPLVPGGVQLSYVLGNDHAYVWARSEAGIVVSVLSKTPHALEQELIALGALD
                     RQAAPEKVEQALEQVSAMLLPAGLVPANSTSVDVVAEGRIAGVPFPGLRSSTDSNRRL
                     AETHAITMITSMFAVDEAPRVKHARPFRLVALASGSGSLRSAAIPDPAPKLQAATKEI
                     RIVADLFVARDQNAKIKLLLGAEGSAATLHGIWASGADVVHFATHALADLRQPLASLL
                     VLPATDTAGAPTYLTAGQVEGWRGDAELVFLSACESAIGPPRFAAGLPGLQRAFLRAG
                     ARGVIATLWPIEDVLAQEFSADFYRRYTSGQSAAQSLSETQRAGLAPKPGADAAEQTR
                     RRITALAHGFYAP"
BASE COUNT         1033 a         1912 c         1996 g          994 t
ORIGIN      
        1 ccttcgccgt ccttgagata gccctcacaa tcgaaacgtc cggcatcgcg cgtgatgttg
       61 aactggacgt cgcgtttgcc gctcgcggac aggtcaagac cctgcagcgc gctcgccggc
      121 caatccgatt catggtgcga gctgccgccg tgcatacgat gcatgagcgc aagccgcact
      181 ttgccggctt ccttcgctgg cgcgatcgtc cagtttcccc tgaatgtctc cgcgctcaac
      241 gacgccattg aaaaggcgag agcgcagaag ctcaaggcca gcgagagagc agttgtcttg
      301 atggacttca tgatgggctc ctggccgtgg ctcaaaggat gtcggcgttc ttcaactgga
      361 tgagcttctc gacggtaagg ttcttgaagc catgtgcgtt gacctcccga atgaactccg
      421 gggtgatgtc catgaccttg gcctggatga gttgctgcgt gtccatcttg tagccggcgg
      481 cggccagctt gcgctgatag tcggccgtga ctccgtgcac cttcagcgcg atgagttcct
      541 ggccgtctgg ctggaatccc gcggcgcgaa tctgctgcac gtattcgggc gtgatgtcgt
      601 ggatcttcat cgcgatgatt tcctgcgcgt cgggtttgaa acccgcctcg cgcatgcctt
      661 tgatgtaagc aggagtcacg tcgtgaatct tcatcgcgat cagttcgctg gcgttggggt
      721 tgtagccgga cgagcgaata tcacgaatgt agccaggcgt gacgtcctgc accttgagcg
      781 cgatcagatc gtccacgtcg aggttgtcgt aacccgctgc tctcatttcc tcaatgtagg
      841 aaaccgtggg ggcagtagcg ggcgccttcg gtggcgccag cggtgccgtc ggcgcaagcg
      901 gtgcgagtgg cgcgcgaggc gccacggcag cggccggtgc gaccgcaggg cgcggcgcac
      961 gcgccggctt cggtgcgggc tgttgcacct gctcgacctg ctccacgttc tccacgctat
     1021 tcgcctgtac ggcgacggcg gcttcagcct gtgtgaccag ttgctgcagc gagacgtcgt
     1081 tgccctgcgc tcgcgccggg agcgccacac cgacggtgag cgcgccggtg acgagggcgg
     1141 tggcgagcac cagggtggcc gtgaccatgc ccgccgttcg cgcgcccggc tcctgggtcc
     1201 gcacaccgag aagccgcgcc acgcgcgccg cgaccgaact acccgtggcg gccatcgcga
     1261 agtcgggcac gtcgcgccaa ccttccatgg tggcgagcgc gcgggcgtaa ccgacggtgc
     1321 cgccacacgc ggcaatcgcc acatcgtcac agcagtcttc gcgatccgcg cgaatgcgct
     1381 tgttgagcca ccagatggcg ggatggaaga agaacagcgt ctcggcaatg acctgcacga
     1441 aattgacggc gacatcgaat cgcttgatgt gaccgagctc gtgcgcgatg acggcttcga
     1501 gctgttcggg agaaagcccg gtcagtgccc gcatgggcaa cagcacgacg gggcggaaga
     1561 atccgatgac cgcgggaacg ctcagcagat ggcactcgca gtaacggatg gcgcggtgta
     1621 tgccgagacg atgttgcaag gcttcgaaac gcgcgaccaa cgcgggcggc agcgcgacga
     1681 gattccggcg ccgaagctgc tcgagcacta gaaggccgaa cgcgattcgc agcgcgaaga
     1741 aaaacacacc gacgagccac gcacgcgaga cccagagaaa gtcttcgctc cgaaaagtgg
     1801 ccgcttgctc atccgtagtt agctcatcca cggagttcgc ggttgcctcc agggtagagg
     1861 ccgaggcggc ggccggcgcc tgtgcagtcg gaaaatcggc cggggccgtg gtggattcgc
     1921 cgagcagcga aaacgtcacg ccgaacgaga ccagcatgag cacgagcgcg ccgatgccaa
     1981 ccaggtaacg cgtcgccggc ttgcgcatca cgaacaggag cgacgccgtg accgcggcaa
     2041 tggccgcgcc ttgccacagg aaatggagca gggaccaggc cagcgtctgc atgagagtag
     2101 tagtcgcgtt catggaagtt tcctttcgta ttcgtcgagc atgcggcgca tctcgtcgat
     2161 ttctttcttg gaactgcggc gccccgacag ggcgtgctgc atgagctggc tcgcggagcc
     2221 ggagaagacg cgcttgagca cgtcggccgc gaactgctgc ttggtggttt cggcgggctg
     2281 gtgtgccgag tagatatgag cccgctgctc ttcggtgcgg gtgaccaggc ccttgctggt
     2341 cataatctgg aggaacttga gcgcgctcgt gtatcccatg tccttctctt tcgagaggac
     2401 ctcgtggatc tcccgcaccg tggaagggcc gcgcgaccag aggatttgca ggatcgcgag
     2461 ctcggacggc gtgggctgca gtttggggtc tgtcttcatg gggatcgagg ctactacgaa
     2521 gtatttcgta ctgtcaacga aggtcttcgt acaagcgcta atccgggcca ccctccatta
     2581 agctagctcg cgatcagagg gctcgaaaca cgcatgtcga tcagattctg cgcgccggca
     2641 ctcggcgttc tcttccttgt ggcctgcaca tcgcggccat cgacgcccga tggggcgatg
     2701 cttctcgacg aagaggtgac gcttcgacgc gaagcgaaag tcgacacggc gcaacgtgaa
     2761 atcgaagtcg acagcggctc tattgtcgtg gtgttcgtcg acgagaaact gaccgacgtc
     2821 aaagtcaggc tcgcgatcgt cggcgccgac ggcgaaccgc tcaccgtcga agtggaaaac
     2881 aatctgcgcg gttcgggtct cgaggttgcc gccatcgaag cgccgcgcgg ttcgcgagtc
     2941 tcgatcacgc tgacgggacc gcaggattcg gctgcgccgg gtcatgtaca cctcaacgtg
     3001 cggcgcttcg atgtgcaccc ggaagaatcg aaattcacgg cgcaggtcgc ggcgttcaag
     3061 tcgtggtcgt cggccacgaa cgcaagcctg cgcacggacg cggtcaaggc cacggcgctg
     3121 gccgaaatgg atagagcgat cagcggactc agctcaccac ccggtgacgc agcgctcgcc
     3181 gccaaggcgc gaatcgtcaa ggcgaacatg ctgttgttct tccgcgtgga ctggcgcgaa
     3241 tcacgcgccg aggcgcaacg cgcggcggat gcgttcggcg cgctgcccaa ggtcgacaca
     3301 ctcaatgtgt cgcgcgcgaa actcatcgag tcgatggcgc tgctgcagat cgcggcggac
     3361 agaaaggcca cgaacccgac ttccgaggaa gccgtgtctc tggcgcgcgg gatcctcgcg
     3421 gggctcatcg atccgctgtc gatgttcgac cctgtcgaac gcgcccgcgc gatcacggtg
     3481 ctggggcagg tggactacgc caccacgcgg ctcgacgacg cggacaagcg ttacgccgaa
     3541 gcccaggcta tctaccaggc cgccggctac gctgcgggcg agcgtgaaat acgttgccac
     3601 ctcgcggtgg tgttactcga gcgcggccgc ttccgtgacg cggcgctggc attcgtggcg
     3661 ctgctcccgg aagtcgacga gatcgcgagc cctgagatgc gcgtcaccgt gctgctcggc
     3721 gcggcgcgtg gccaatcctt ctccgcgcgt accgatccgg ccgcggagtt gcagatccgc
     3781 gcgctggccg tggctcgcga gtaccagttg cgcgacaagg aagcgagcgc gctgcacgga
     3841 ctcggtcacg tgtacgccaa ccgtggcgac ccgctgcagg cacgtgcgta ctacgccgag
     3901 gcgctgcgtg ccgcgcgtaa cggatcggac gtcatgagct acgtgtgggc actcgcatcc
     3961 gcaggcgtgg tcgcgcggga cgacggcgac cacgaccagg cggtcgcgct gcacaaggag
     4021 gccgtgagcc tcgcatcgaa tccgatcgcg cgcgtgcgca cgctgcgtga gctggggctc
     4081 gactactatt tcgcacgcga cttcgcggcg gccatcgtgg agtttcgtct cgccctcgat
     4141 gtcgacctgc acgatcccgt tcaccacgcg tacagcgacg tgaagcgcaa tctcgcgcag
     4201 gcgctcatcg agaccgacga tccgtcgccc gccacgttca aggaagccgc gcggctcctc
     4261 gacgaatcgt tgaaaagctg cctgcgtgtc ggcgacaagc agggcgagat aggcgcgcat
     4321 cgcatcaccg gccagctgct ggcgaaccag ggcaggtcca aagaggcgct cgtcgagtac
     4381 gaacgcacgt tcgcgctcgc cgacgcctat cgcgggagca gtgcgagccc cgaagcgcgc
     4441 cgcgccacgc tgccgcacgt gcagcaggcg ttccgcggct atctcgatct cgagatgaag
     4501 tccgtggtgg cgcgcggcgc gggcaaggtg cagcccgcgt cgaaccgcga aatggccgcg
     4561 ctgcgcaaac tcgaaatggc ccgcgacgcc aacttcggcg ccgcgcgcac gggcgagctg
     4621 gacgtcgaga cggccagaca ggtcgacgcc ctgctgtcgc agatggctaa caaaagcctg
     4681 gcgatcacgg cgatgctcaa gcgtgagctt gcgaaatccg aagaggcgga gctcgcctcc
     4741 ttgcagtccg gcatggccga tcttcacgcg gagctcgatc gcctgcgcac ggctgcggcg
     4801 accaaggccg cggttgcgga gaaggcccag gccatcacga cgcgcgactg gcgtccgctg
     4861 gttccgggag gcgtgcagct ttcgtatgtg ctcggcaacg atcacgccta cgtctgggcg
     4921 cgtagcgagg ccggcatcgt cgtgtcggtg ctgagcaaga caccgcatgc actcgagcag
     4981 gagttgatcg cgctcggcgc gctcgaccgc caggcggcgc ccgagaaagt cgagcaggcg
     5041 ctggaacagg tttccgcgat gttgctgcct gccggcctcg tccctgccaa ttccacatcg
     5101 gtcgacgtgg tcgccgaagg ccgcatcgcc ggcgttccgt tcccagggct gcgatcgtcc
     5161 acggattcga accggcgact cgcggagacg cacgcgatca cgatgatcac ctcgatgttc
     5221 gcggtcgatg aggcgccgcg cgtgaaacac gcgcggccct tccggctggt ggcgctcgcc
     5281 agcggcagcg gctcgctgcg ctcggcggcg atccccgacc cggcgccgaa gctccaggcc
     5341 gcgaccaagg aaattcgcat cgtggccgac ctgttcgtgg ctcgggacca gaatgccaag
     5401 atcaaactac tgctcggcgc ggaaggcagc gccgcgacgc ttcacgggat ctgggcgagc
     5461 ggcgccgatg tcgtgcattt cgccacgcat gcgctcgccg acctgcgcca gccgctggcc
     5521 tcgctgctcg tgctgccggc gaccgacacc gccggcgcgc ctacctacct gaccgcggga
     5581 caggtggagg gctggcgcgg cgatgcggag ctcgtgttcc tgagcgcctg cgaaagcgcg
     5641 atcgggccgc cgcgtttcgc agccggcctg cccgggctgc agcgcgcctt cctgcgagcc
     5701 ggcgcacgtg gcgtcatcgc gaccttgtgg cccatcgaag acgtgctggc ccaggagttc
     5761 tccgcggatt tctaccggcg ctacacgagc ggacagtccg cagcgcagtc gctgagcgag
     5821 acgcagcgcg cggggctcgc gcccaagccg ggcgcggacg ccgccgagca gacccgccgc
     5881 cgcatcacgg cactggctca cgggttctac gcgccctagc agccgcgcga gcttt
//
LOCUS       DATDEU010000021         2738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1608099, whole genome shotgun sequence.
ACCESSION   DATDEU010000021 DATDEU010000000
VERSION     DATDEU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608099"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="pth"
                     /locus_tag="VIV63_00405"
     CDS             <1..540
                     /gene="pth"
                     /locus_tag="VIV63_00405"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY6123081.1"
                     /translation="HNAGFWLVDELARRHGGAFRFEGKHQAELARIRIGGEELWLAKP
                     MTFMNRSGGPVSSVLGFYKMTPAQMLVAHDEIDLPSGTVRLKEAGGHGGHNGLRDLIA
                     AQGDGFWRLRIGVGHPGTKNEVVDFVLTRAGADEQRAIDETIVAGADAIEQMLRSGAQ
                     IAMNKLHSLGKPAANAKSE"
     gene            545..1636
                     /gene="ychF"
                     /locus_tag="VIV63_00410"
     CDS             545..1636
                     /gene="ychF"
                     /locus_tag="VIV63_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY6123082.1"
                     /translation="MGIKCGIVGLPNVGKSTLFNALTRAQIAAENYPFCTIDPNVGIV
                     PVPDLRLDALSKIVSPEKIVPTTVEFVDIAGLVAGASQGEGLGNKFLAHIRETDAISH
                     VVRCFEDADIVHVSGRVNPISDIDIIDTELALTDLQSVEKAVDRNTKAAKGGDKDAAR
                     KKDLFERMRKHLDDGKPVRSMGLTKDEKADSQELHLLTAKPVMYVANVKEDGFTNNPH
                     LDAVTERAKAEGAVVVAVCAAIEAEISQLEEADRADFLRDLGLAEPGLNRVIRAGYEL
                     LGLQTYFTAGVKEVRAWTVRGGSTAPQAAGVIHTDFERGFIRAEVIAYDDFIKYQGEA
                     GAKDAGKLRLEGKEYLVKEGDVMHFRFNV"
     gene            1667..>2738
                     /locus_tag="VIV63_00415"
     CDS             1667..>2738
                     /locus_tag="VIV63_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6123083.1"
                     /translation="MLARVRKWLRRLGFALGIVIATILFVRAFDAWRSPPLKLWHTQV
                     PHELDADAIDAADWESWLTAENAAFAEMQSEVIDALPAEDRIPIDRYFVDSPINPQRF
                     PQNWNHSYVLTPMSAPRGAVVLFHGLTDSPYSMRHIAMRYVEHGFVAVAVRMPGHGTV
                     PAGLTTTRWEAWAAATRLAVRTARELAGDNVPLHVVGYSNGGALALKYSLDSLSNPKL
                     ARPDRLVLISPMVGITSFARFAGVLGWPAAFPSFAKAAWLDVLPEYNPFKYNSFPVNA
                     ARQSSQVVRAVEEEFRRAAESGVLAKLPPILTFQSTLDSTVSTGAVVNSLYANLPANG
                     SELVLFDRNHRAGVGPMIRPAQA"
BASE COUNT          516 a          842 c          915 g          465 t
ORIGIN      
        1 cacaacgcgg gcttctggct cgtggatgaa ctggcgcggc ggcatggcgg tgcgttccgg
       61 ttcgaaggca agcaccaggc agaactggcg cgcatacgaa tcggcggcga ggagctttgg
      121 ctcgcgaagc cgatgacctt catgaaccga agtggcggac ccgtgtcgag cgtgctcggc
      181 ttctacaaga tgacgccggc gcagatgctg gtggcacatg acgaaatcga tctgccgagc
      241 ggaacggtac ggctcaagga agccggcggc cacggtggac acaacggcct gcgcgatctc
      301 atcgcggcgc agggcgatgg cttctggcgg ctgcgcatcg gcgtgggcca tccgggaacg
      361 aaaaacgaag tcgtggactt cgtgctgacg cgcgccggcg cggacgagca acgcgcgatc
      421 gacgagacca tcgttgcggg cgcggatgcg atagaacaga tgctgcgctc cggagcgcag
      481 atcgccatga acaaactaca ttcgctcggc aagcccgcgg cgaatgcgaa atcggagtag
      541 ctggatgggt atcaagtgcg gcatcgtcgg tctgcctaac gtgggcaagt ccacgctgtt
      601 caatgcgctg acgcgcgcgc agatcgcggc ggagaattat cctttctgca ccatcgaccc
      661 gaacgtcggc atcgtgccgg tgccggacct gcggctcgac gcgctgtcga agatcgtctc
      721 ccctgagaag atcgtgccga ccacggtcga gttcgtcgac atcgcgggcc tggtggccgg
      781 cgcgtcgcag ggtgaaggtc tcggcaacaa atttctcgcg cacatccgcg aaaccgatgc
      841 gatctcgcac gtggtgcgct gcttcgagga cgcggacatc gtgcacgtgt cggggcgcgt
      901 gaatccgatt tcggacatcg acatcatcga caccgaactc gcgctcacgg atctgcagtc
      961 ggtggaaaag gccgtcgacc gcaataccaa ggcggccaag ggtggcgaca aggacgcggc
     1021 gcgcaagaag gatctgttcg agcgcatgcg caagcacctc gacgacggca agccggtccg
     1081 atcgatgggc ctcaccaagg acgaaaaagc tgacagccag gagctgcacc tgctcacggc
     1141 caagcccgtg atgtacgtgg ccaacgtgaa ggaagacgga ttcacgaaca acccgcatct
     1201 cgacgccgtg acggagcgcg ccaaggccga aggcgccgtg gtcgttgccg tgtgcgcggc
     1261 catcgaagcg gagatcagtc agctcgaaga agccgaccgc gcggatttcc tgagagacct
     1321 cggcctcgcc gagcccggcc tcaaccgcgt gatccgcgcg ggatatgaat tgttaggcct
     1381 gcagacctac ttcaccgcgg gcgtgaagga agtgcgtgcc tggacggtgc gcggaggatc
     1441 gacagcgccg caggcagcgg gcgtcatcca cacggatttc gagcgcggct tcatccgtgc
     1501 tgaagtcatc gcctacgacg acttcatcaa gtaccagggc gaagcgggcg cgaaggatgc
     1561 cggcaagctg cgtctcgagg gcaaggaata cctggtgaag gaaggcgacg tgatgcactt
     1621 ccgcttcaac gtctagttcg cgaggcagcg cctcggggag atgagcgtgc tggccagagt
     1681 cagaaagtgg ttgcgccgat tgggcttcgc gctcggcatc gtgattgcca ccatcctctt
     1741 cgtgcgcgcc ttcgacgcat ggcgctcgcc gccactcaag ctctggcaca cgcaggtgcc
     1801 gcacgaactg gacgccgatg ccatcgacgc cgcggactgg gaatcctggc tcacggcgga
     1861 gaatgccgcg tttgccgaaa tgcagagcga agtgatcgat gcgctgccgg cggaagaccg
     1921 cattcccatc gatcgctact tcgtcgacag tccgatcaat ccccagcgtt ttccgcagaa
     1981 ctggaatcac tcgtacgtac tcacgccgat gagcgcgccg cgcggcgccg tggtgctgtt
     2041 tcacgggctc acggattcgc cctacagcat gcgccacatc gccatgcgct atgtcgagca
     2101 cggttttgtc gcggtggcag ttcgcatgcc gggtcacgga acggtgccgg ctggcctgac
     2161 taccacgcga tgggaagcct gggccgcggc gacgcggctc gccgtgcgta cggcgcgaga
     2221 gctcgcgggg gacaacgtgc cgctgcacgt ggtcggctat tcgaatggcg gcgcgctggc
     2281 gctcaagtat tcgctcgatt cgctgtcgaa tccaaaactc gcgcggcccg accggctggt
     2341 gctcatctca cccatggtgg gtatcacgag cttcgcgcgt ttcgcgggcg tgctcggctg
     2401 gccggccgcg ttcccgtcgt tcgcgaaagc ggcatggctg gacgtgctgc ccgagtacaa
     2461 cccgttcaag tacaactcgt ttccggtgaa cgccgcgcgg cagagttcgc aggtcgttcg
     2521 tgcggtggag gaagagttcc gccgcgccgc ggagagtggc gtgctcgcca aactaccgcc
     2581 gatactcacg tttcaatcga cgctcgactc gacggtttcc accggcgccg tcgtcaattc
     2641 gttgtatgcg aacctgcctg cgaatggcag cgagctggtg ctcttcgatc gcaatcatcg
     2701 tgccggcgtt gggccgatga tccgtcccgc gcaggcgg
//
LOCUS       DATDEU010000022         8033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_587453, whole genome shotgun sequence.
ACCESSION   DATDEU010000022 DATDEU010000000
VERSION     DATDEU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587453"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="VIV63_00420"
     CDS             <1..1574
                     /locus_tag="VIV63_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HEY6123084.1"
                     /translation="SAPSELVGPDFHLTIDELPVNYTGAARRAVAVNGQVPAPLLRMR
                     QGDTVTIRVTNNLRESTSIHWHGIILPADMDGVPGLSFDGIAPGTTFTYRFKVNQSGT
                     YWYHSHSRFQEQVGLYGPIVVERRGGERHGADREHMLLLSDWTDHDPEHIYATLKRQS
                     DYYNFGKRTFGDFVDDVRKQGFSAAAADRRMWGAMRMNPTDLADVTGYAYTYLLNGAT
                     PAANWTGVFKPGERVRLRLINGSSMSFFDVRIPGLKLTVVATDGQDVEPVTVDELRIG
                     TAEVYDVIVTPGDAAYTVFAQAMDRSGFARGTLAPRAGMTAEVPALDARAILTMNDMG
                     MSHAGMDHSQMDHSAMPKVNHAPAEAGPIVDMRADMPNTRLDDPGVGLRDNGRRALTY
                     ADLATLGGPIDRREASREIELHLTGHMERYIWSFNGQKFSDAQPLRFTHNERLRIVLV
                     NDTMMSHPIHLHGMWSELVDAQGKFVARKHTVIVQPGQRLAYEVTADALGSWAYHCHL
                     LYHMEAGMFREVVVA"
     gene            1598..2362
                     /locus_tag="VIV63_00425"
     CDS             1598..2362
                     /locus_tag="VIV63_00425"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEY6123085.1"
                     /translation="MFALAAQALGQEPGLSEREHVPPDPPRTRVHAMPYHEMADMMGM
                     DDRKRFGKVMIDRLEWRDADTSIYEWDAAAWYGGDFHKLWIETEGEHADGETHDARVE
                     VLWDRIASAWWSTRLGLRHDFGEGGTQSWLAFGVAGLAPGFVELDATAYLGDGGRAAM
                     RVSADYDLLLTQRLVLQPEVEVNLYSEDDPERLIGAGLSDLELGLRLRYEIRREFAPY
                     LGVSWSKRFGDSADLASAAGEETDEISLVAGFRLWF"
     gene            2433..2897
                     /locus_tag="VIV63_00430"
     CDS             2433..2897
                     /locus_tag="VIV63_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY6123086.1"
                     /translation="MKAPYRERLSDFPESMGVPAGRAGVRTEKVTFSDDRRARGSRAL
                     AEITGESGSAVVNSLRDIAPDLADWIVDFSYGDVMARPGLDLRSRQFATIAALTALGN
                     AQPQLKVHIAGALNVGCKPQEIVEVIMQMAVFAGFPAAINALTVAREVFATR"
     gene            complement(3217..4146)
                     /locus_tag="VIV63_00435"
     CDS             complement(3217..4146)
                     /locus_tag="VIV63_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY6123087.1"
                     /translation="MRSLCVLVAALLAACSGDRPPVVEVQPAEVATPAMEAQPTVTET
                     SAGPVNAALVFRVIREGGGGVDATINEVDRQGQASFYATVPGTGEKALDKPCEAGQRF
                     QAEPKVAAFLRGPTQDCAERVEFMLYSTQATLQFIKRGDDATAGGDLLVAQSNYGTAA
                     DRLQYAEPEKARQLRVLSTVAAGRALGVVEPVKGAGGSEQPSREFKDRVRVFQRQNGI
                     EATGELDARTRDSIGRMQLRGGAVVVPPAASSGALSPTSVASVAAAPALVVTPQAVTP
                     AVEVRVTAVELLATPASPQNVAVIHANRARSVP"
     gene            complement(4139..4762)
                     /locus_tag="VIV63_00440"
     CDS             complement(4139..4762)
                     /locus_tag="VIV63_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123088.1"
                     /translation="MGWWRRFFNGLLSKDKSLATGLILGFMAWTLMRLVDGIAANGTI
                     EYDIANRPATLADGRSGYVVRVTLTNLSADTALTNLKASIAAPSADIVFSADPKDRSC
                     AFEPPGWGGQPTCDAFASGFDFLAPMIVAGTHVQFEVKYTRPDAVTALPVVRIKPDTT
                     KFRLVEPGFSTFVARNQVGILLALLAFALVTFFLSVAAGVPKGEPHA"
     gene            complement(4845..6203)
                     /locus_tag="VIV63_00445"
     CDS             complement(4845..6203)
                     /locus_tag="VIV63_00445"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY6123089.1"
                     /translation="MNEKMPPPVGRLSHEESAGYDRRVIDYIQRASATGLYEIRGLGA
                     KRHLPSFDDLVFLGASLSRYPLEGYREKCSTRTVLGTRHATKPIELATPITIAGMSFG
                     ALSARVKEALGRAATALGTSTTTGDGGMTSEERESSKILVYQCLPSRYGFNPDDVRRA
                     DAIEIVIGQGAKPGGGGMLLGQKVNPRVAAMRTLPAGVDQRSASRHPDWTGPDDLVIK
                     INELREITDWEKPIYVKVGATRVNHDVKLAIHAGADVIVVDGMQGGTAATQTVFIEHV
                     GIPTLAAVRQAVEAIDDLNMRGKVQLIVSGGIRTGADVAKALAMGADAVAIGQAVLMA
                     LGCNSDSYLVGDNHVSAVEDYARLGTAPGFCHHCHTGRCPVGVTTQDPVLEQRLDPLS
                     GARRLTNYLKTLNMELTTLARACGKQDVHHLEREDLVALTVEAAAMANLPLAGTNWVP
                     GK"
     gene            complement(6213..6896)
                     /locus_tag="VIV63_00450"
     CDS             complement(6213..6896)
                     /locus_tag="VIV63_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123090.1"
                     /translation="MKVFDLATTSLREVNRYLHRDAADAGVKRVKILNADGAHSIAAG
                     VNVPLDVEIDGHAGYYAGGMNKHANITVHGNAGPGVGENMMSGTIRVEGFASTAAGAS
                     AHGGTLVILGDAALRCGISLKGGDIVVGGDVGSFSAFMAQAGRIVICGDAGDALGDSL
                     YEAVIYVRGQVRSLGADARFEPMTDADYAAVRELLGRAGLDHDPKTFQRVASAKGLYH
                     WNSDANQEY"
     gene            complement(6893..7786)
                     /locus_tag="VIV63_00455"
     CDS             complement(6893..7786)
                     /locus_tag="VIV63_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY6123091.1"
                     /translation="MCGIVGLLLKKPELRDQLGELMTPMLVGMTSRGPDSAGVAIFGS
                     ALTDAHKLSLFWGEGAADWKRLGAELSAEFDGKHRLAPTGQHAVLVTPANPEEVKAWL
                     GEAAPEIYVLSMGRSIDLYKDIGAPADIVERYALNKVTGTHVVAHTRMATESAVTPAH
                     AHPFTAGQDFCLVHNGSLSNPHLVRKKLEPLGIRFVTDNDTEAACRFFEWRMREGDDL
                     TTAVHRGFSELDGFFTFLMGTDKEMLIVRDAFACKPAVVAETDDYVAIASEFRSLAHL
                     PGVRHASVFEPKPEEVYSWKA"
     gene            complement(7786..>8033)
                     /locus_tag="VIV63_00460"
     CDS             complement(7786..>8033)
                     /locus_tag="VIV63_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123092.1"
                     /translation="PGEPTNLNMYELSEAQLQEKKISLLPQNLLEAVGALEADDVVKA
                     GLGGELAAEFIKLKRMEWTEYARHVSDWETKRYLEFF"
BASE COUNT         1360 a         2661 c         2617 g         1395 t
ORIGIN      
        1 gcagcgcgcc ctctgagctc gtcggcccgg acttccatct gaccatcgac gaactgccgg
       61 tgaactacac gggcgccgcc cgccgcgccg tggccgtgaa cggccaggtg ccggcgccgc
      121 tgttgcgcat gcgccagggc gacacggtga ccatccgcgt cactaacaat ctgcgcgaga
      181 gcacctcgat ccattggcac ggcatcatcc ttccggccga catggatggc gtgcccgggc
      241 tgtcgttcga cggcattgcg ccgggaacga cgttcaccta tcgcttcaag gtgaaccagt
      301 ccggcaccta ctggtaccac tcgcactccc gattccagga gcaggtcggg ctgtatggcc
      361 cgatcgtcgt cgagcgccgc ggcggcgagc ggcacggcgc cgaccgcgag cacatgctgc
      421 tgctgtcgga ctggacggat cacgatcccg agcacatcta cgccacgctc aagcgccaga
      481 gcgactacta caacttcggc aaacgcacgt ttggcgattt tgtcgatgat gtgcgcaaac
      541 agggtttctc ggccgcggcc gcggaccgac gaatgtgggg cgcgatgcgc atgaatccca
      601 ccgatctcgc ggacgtcacc ggatacgcct acacctatct attgaacggc gccacgccgg
      661 cggcgaactg gacgggcgtc ttcaagcccg gcgaacgcgt gcggctgcgc ctcatcaacg
      721 gctcgtcgat gagtttcttc gacgtgcgga ttccgggcct gaagctcacg gtggtggcca
      781 ccgacggcca ggacgtcgag cccgtcaccg tcgacgaact gcgcattggc accgccgagg
      841 tttatgacgt catcgtcacg ccgggtgacg ccgcgtacac ggtgttcgcg caggccatgg
      901 atcgctccgg attcgctcgc ggcacgctgg cgccccgcgc gggcatgacg gccgaagtgc
      961 cggcgctcga tgcacgcgcg atattgacga tgaacgacat gggcatgagc cacgccggca
     1021 tggatcacag tcagatggat cactcggcga tgccgaaggt gaaccacgcg cccgccgagg
     1081 ccggccccat cgtcgacatg cgcgccgaca tgcccaacac acggctcgac gatccgggcg
     1141 tgggcctgcg cgacaacggc cggcgcgcac tgacctatgc cgacctcgcc acgctcggcg
     1201 gccccatcga ccggcgcgag gcgtcacgcg aaatcgagct gcatctgacc ggccacatgg
     1261 agcgctacat ctggtcgttc aacggccaga agttttccga cgcgcagccg ttgcgattca
     1321 cgcacaacga acgcctgcgc atcgtgctgg tgaatgacac catgatgtcg catcccatcc
     1381 acctgcacgg catgtggagc gagctggtgg atgcgcaggg aaagttcgtg gctcgcaaac
     1441 acacggtcat cgtgcagccg ggacagcgac tggcgtatga ggtcacggcg gacgcgctgg
     1501 gtagttgggc ctaccactgt cacctgctct atcacatgga agcgggcatg ttccgcgaag
     1561 tggtggtggc gtgaggcgcg cgtgcgcagg cgtgatgatg ttcgcactcg cggcccaggc
     1621 attgggccag gagcccggcc tcagcgaacg cgaacacgtg ccgccggatc cgccccggac
     1681 gcgcgtacat gcgatgccct atcacgagat ggcggacatg atgggcatgg acgaccgcaa
     1741 gcgcttcggc aaggtcatga tcgaccggct cgaatggcgc gacgcggaca cctctatata
     1801 tgagtgggac gccgccgcgt ggtacggcgg ggacttccac aagctgtgga tcgagaccga
     1861 aggcgaacac gcggatggcg aaacccatga cgcgcgcgtg gaagtcctgt gggatcggat
     1921 tgcctccgcg tggtggagca cgcgcctcgg cctgcgtcac gatttcggcg aaggtggtac
     1981 gcagagttgg ctggcattcg gagtcgctgg cctcgcgccc gggttcgtcg agctggacgc
     2041 gacagcctat ctaggcgatg gcggacgtgc ggcgatgcgc gtgtccgccg attacgatct
     2101 gctgttgacc cagcggctgg tgctgcagcc cgaagtcgag gtcaatctct acagcgagga
     2161 cgatcccgag cggctcatcg gggccggact gtcggacctg gaactggggc tgcggctgcg
     2221 ctacgagatt cgtcgcgaat ttgcgccata cctgggcgtg agctggtcga agcgattcgg
     2281 cgacagtgcc gacctggcga gcgcggcggg tgaggaaacc gacgaaatct cgctcgtcgc
     2341 aggttttcga ctctggttct agcggttgtc tgacagcaag gtcggcgcgc cgccgcgtgc
     2401 ctgcgcgagg gctgtcgaat aggcttcgcg ccatgaaagc cccctaccgc gaaagattgt
     2461 ccgacttccc cgaatcgatg ggtgtgcccg ctgggcgcgc tggcgtgcgc accgaaaagg
     2521 tgaccttttc cgacgatcgc cgcgcgagag gatctcgcgc gttggcggag atcaccggcg
     2581 aatcaggatc ggcagtggtg aattcgttgc gcgacatcgc gcccgacctg gcggactgga
     2641 tcgttgattt ttcgtatggg gatgtcatgg cccgtcccgg gctcgacctg cgctcgcggc
     2701 aattcgcgac catcgcggcg ttgacagcgc tcggcaatgc gcagcctcaa ttgaaagtgc
     2761 acatcgccgg cgcgctcaac gtcggctgca agccgcagga aatcgtcgaa gtgatcatgc
     2821 agatggcggt gttcgcggga ttccccgcag ccatcaacgc gctaaccgtt gcgcgcgagg
     2881 ttttcgccac ccgttgaaat ggggacattc ctgatttgct agcaaaacgg gacgcggctc
     2941 aatcgaggag cgtcatcgcg agacggacaa gcttccggcg ctcagagtcg ggcgcgcttc
     3001 gcgttcgctc gcgctcggcg gcgcgtccag agctgtcagc gcttcgttat cgggtggaca
     3061 atcactacgg tgtctcgagg aaccaccagg agtacctgcg cgcttagcgc ctcgcgctcg
     3121 ccgccctcca cttcgcgccg gaagcttgtc cgtctcacgc tgacgcaccc acttccagcc
     3181 ccgtcccctt ttgctagcaa atcaggaatg tccccattac ggcaccgagc gcgctcggtt
     3241 cgcgtggatc actgcaacgt tctgcggcga cgccggagtg gctaacaact ccaccgcggt
     3301 cacgcgcact tccaccgccg gcgtcaccgc ctgcggcgtg acgacgagcg cgggcgcggc
     3361 ggccaccgac gccacactgg tcgggctcag cgcacccgat gacgcagccg gcggcacgac
     3421 cacggcgccg cctcgcaatt gcatacgccc gatcgagtcg cgcgtgcgcg catcgagctc
     3481 gcctgtcgcc tcgatgccat tctgccgctg gaacacgcgc acgcgatcct tgaactcgcg
     3541 cgaaggctgc tcactgccgc ccgctccttt caccggctcc acgacgccga gcgcacggcc
     3601 ggccgcgacc gtggacaaca cgcgcagctg ccgcgccttc tccggctccg cgtactgcag
     3661 acgatccgcc gcggtgccgt agttggactg agccaccagc aggtctccac cggccgtggc
     3721 gtcgtcgccg cgcttgatga actgcagcgt cgcctgggtg ctgtagagca tgaattccac
     3781 gcgctcggcg caatcctgcg tgggtccgcg caggaacgca gccaccttgg gctcggcctg
     3841 gaatcgctgc ccggcttcgc agggtttgtc gagcgccttc tcgcccgtgc ccggcacggt
     3901 cgcgtagaag ctggcctgac cctggcggtc gacctcattg atcgtggcgt cgacaccgcc
     3961 gccgccttcg cgaatcaccc gaaataccag tgcggcattc accggtcccg cggatgtctc
     4021 cgtgaccgtc ggctgcgctt ccatcgcagg cgtcgcgacc tcggccggtt gcacttcgac
     4081 gacgggaggc cggtcgccgc tgcacgcggc cagcaacgcg gcgacgagga cacacaggct
     4141 acgcatgagg ctctcctttg ggcactccgg cggcgacact cagaaaaaag gtgacgagcg
     4201 cgaatgccag cagcgccagc aggattccca cctggttgcg cgcgacgaac gtcgaaaagc
     4261 cgggctcgac gagccggaac ttcgtggtgt cgggcttgat gcgcacgacg ggcagcgccg
     4321 tcacggcatc cggccgcgtg tacttcactt cgaactgcac atgcgtgccc gcgacgatca
     4381 tgggcgccag aaaatcgaag ccgctggcga acgcgtcgca cgtcggctgg ccgccccagc
     4441 ccggcggttc gaacgcgcag ctccggtctt tcggatcggc gctgaagacg atgtcggcgc
     4501 tgggcgcggc gatcgacgcc ttgagattcg tgagtgcggt gtccgcggaa aggttggtga
     4561 gcgttacgcg caccacgtac ccggagcggc catcggccag cgtcgccgga cggttcgcga
     4621 tgtcgtattc gatggtgccg ttggcagcaa tgccgtcgac cagacgcatg agcgtccagg
     4681 ccatgaagcc caggatgagg cctgttgcca gggacttgtc cttggacagc aatccgttga
     4741 agaaacgtcg ccaccaaccc atgcgggatt ctcaactggc gacggggtgg atcacgcgcc
     4801 cactcgccgg tcagcgggca aaagtgtgaa caattcctgc gttgctactt tcccggtacc
     4861 cagttcgtgc ccgccagcgg caggttggcc atggcggcgg cttcgacggt gagcgccacc
     4921 aggtcctcgc gctcgagatg gtgtacgtcc tgcttgccac aggcacgcgc gagcgtggtc
     4981 aactccatgt tgagcgtttt caggtagtta gtaaggcgcc tggccccact gagtggatcc
     5041 agccgttgct cgagcaccgg atcctgggtg gtcacgccca cggggcagcg gccggtgtgg
     5101 cagtggtggc agaacccggg ggccgtgccg agccgcgcgt aatcttcgac cgccgaaaca
     5161 tggttgtcgc caacgagata actgtcgctg ttgcagccca gcgccatgag caccgcctgt
     5221 ccgatggcga ccgcgtccgc acccatggcc agcgccttgg ccacgtccgc gcccgtgcgg
     5281 atgccgccgg agacgatgag ctggaccttg ccgcgcatgt tgagatcgtc gatggcctcc
     5341 accgcctggc gcacggccgc cagcgtcgga atgccgacgt gttcgatgaa cacggtctgc
     5401 gtcgcggccg taccgccctg catgccgtcg acgacgatca catccgcgcc ggcatgaatc
     5461 gccagcttca cgtcgtggtt cacgcgcgtg gcgcccactt tcacgtagat gggcttttcc
     5521 caatccgtga tctcgcgcag ttcgttgatc ttgatgacca ggtcgtccgg cccggtccag
     5581 tccggatgcc gcgacgccga gcgctggtcg acgcccgcgg gcaatgtgcg catcgcggcg
     5641 acgcgcgggt tgaccttctg gcctaacaac atgccgccgc cgccgggttt cgcgccctgg
     5701 ccgatgacga tctcgatggc atcggcgcga cgcacgtcgt cgggattgaa tccgtaccgt
     5761 gacggcaggc actggtacac caggatcttg gagctctcgc gctcctcgga cgtcattcct
     5821 ccgtctccgg tggtcgtcga agtcccgagc gcggtggctg cgcggcccaa cgcctccttc
     5881 acgcgcgccg acagcgcgcc gaagctcatg ccggcgatgg tgatgggcgt ggcaagttcg
     5941 atgggcttgg tggcgtgccg cgtgccgagc accgtgcgcg tcgagcattt ctcgcgatat
     6001 ccctcgagcg gatagcgcga cagcgaggcg cccagaaaca cgagatcgtc gaacgacggg
     6061 agatgccgct tcgcgccgag cccgcgaatt tcgtacagac ccgtcgccga tgcacgctgg
     6121 atgtagtcga tgacgcggcg gtcgtagccc gcggactcct cgtgggagag ccgcccgacc
     6181 ggcggtggca ttttctcgtt catcgctccc cctcaatatt cctgattcgc atcggaattc
     6241 cagtgataca ggcccttcgc cgaggccacg cgctggaagg tcttcggatc gtggtcgagg
     6301 ccggcgcggc ctaacaactc tctgacggcg gcatagtcgg cgtcggtcat gggctcgaag
     6361 cgcgcgtcgg cgccgagcga gcgcacctgg ccgcgcacgt agatcacggc ctcgtataac
     6421 gagtcgccga gcgcatcgcc cgcgtcaccg cagatcacga tgcgtccggc ctgcgccatg
     6481 aacgcggaga aactgccgac gtcgccgccc acgacgatat ccccaccttt gagagagata
     6541 ccgcaacgca gagcggcatc gccgaggatc acgagcgtgc cgccgtgcgc cgatgcgccc
     6601 gcggccgttg acgcaaaacc ttcgacgcga atcgtccccg acatcatgtt ctcgcccacg
     6661 cccgggccgg cgttgccgtg caccgtgatg ttggcgtgtt tgttcatgcc gcccgcgtag
     6721 tagccggcat ggccgtcgat ttcgacgtcc agcgggacgt tgacgccagc cgcgatggaa
     6781 tgcgcgccgt cagcgttgag gatcttcacg cgtttcacgc cggcatcggc cgcatcgcga
     6841 tgcagatagc gattcacttc gcgcagcgaa gtcgtcgcca gatcgaatac cttcacgcct
     6901 tccacgagta cacctcttcg ggcttgggct cgaacacgct ggcgtgacgc acgcccggca
     6961 gatgcgccag cgagcggaac tccgaagcga tggccacgta gtcgtccgtc tccgcgacca
     7021 ccgcgggttt gcaggcgaag gcgtcgcgca cgatgagcat ctccttgtcg gtgcccatga
     7081 ggaacgtgaa gaagccgtcg agctcggaga aaccgcgatg caccgcggta gtcagatcat
     7141 caccttcgcg catgcgccac tcgaagaagc ggcaggcggc ctcggtgtcg ttgtcggtga
     7201 cgaagcggat gccgagcggc tcgagcttct tgcggaccag gtgaggattg gacaatgagc
     7261 cgttgtgcac caggcagaaa tcctggccgg ccgtgaacgg atgcgcgtgc gccggcgtga
     7321 ccgccgactc cgtggccatg cgcgtgtgcg cgaccacgtg cgtgcccgtc accttgttga
     7381 gcgcgtaacg ctcgacgatg tccgcgggcg cgccgatgtc cttgtacagg tcgatggatc
     7441 gtcccatcga caacacgtag atttccggcg cggcttcacc gagccaggcc ttcacttctt
     7501 cggggttcgc aggcgtcacg agtacggcgt gttgcccggt gggcgccagg cgatgtttgc
     7561 catcgaactc cgccgacagc tcggcgccca ggcgtttcca gtccgcggcg ccctcacccc
     7621 agaacaacga cagcttgtgg gcatccgtca gcgcgctgcc gaagatggcg acgccggccg
     7681 agtccgggcc gcggctggtc atgcccacga gcatgggtgt catgagctcg cccaactgat
     7741 cgcgaagttc tggtttcttg agcagcaggc cgacgatgcc gcacatcaga agaactccag
     7801 gtagcgcttg gtttcccagt cggacacgtg gcgcgcgtat tcggtccatt ccatgcgctt
     7861 cagcttgatg aactcggcgg cgagttcgcc gcccagtccg gccttcacca cgtcatcggc
     7921 ttcgagtgcg ccgacggcct cgagcaggtt ctgcggcaac aacgagatct tcttttcctg
     7981 aagctgcgct tcgctgagct catacatgtt gagattggtg ggttcgcccg gat
//
LOCUS       DATDEU010000023         5387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1025265, whole genome shotgun sequence.
ACCESSION   DATDEU010000023 DATDEU010000000
VERSION     DATDEU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025265"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VIV63_00465"
     CDS             complement(<1..751)
                     /locus_tag="VIV63_00465"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEY6123093.1"
                     /translation="MNRPTAIYSAAQVRALDAWEIEKRRVPGFTLMTRAAEGALKVLR
                     ARWPQARRVAVVCGPGNNGGDGYVLARLAQLAGLEALVLAAAPPDKLGGDARRAMEEW
                     LAAGGSAHPFVADALSGSDVIVDGLLGIGLAGAPRAETLAVIRAINAAKRPVMALDIP
                     SGVDADSGAVHEAAVRAEVTVSFVALKSGLFLGAGPEHSGVVLLDDLGVVAPATPQFA
                     PLMRRIDEGELASALPRRARESHKGTHGHVLV"
     gene            774..1853
                     /gene="queG"
                     /locus_tag="VIV63_00470"
     CDS             774..1853
                     /gene="queG"
                     /locus_tag="VIV63_00470"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY6123094.1"
                     /translation="MTFPAPTRDSLLDLKGALAALARERGFDALGVSHVHLSADAAHL
                     DRWLDHGKHGEMGYMAKHGTRRTDPSELVPGTVRVLSARMNYWPPDARDASEALGDPE
                     SGYVSRYALGRDYHKLLRKSLQGLADDIVQRIGPMGYRVFVDSGPVLEKALARNAGLG
                     WIGKHTNLIARDAGSYFFIGEILTDLPLPVDEAATAHCGTCSACIPACPTGAITAPNQ
                     LDARRCISYLTIELHGAIPVEFRRMLGNRIYGCDDCQIVCPWNKFARAAAHPDFKTRH
                     RLDSSRLDELFAWTEVEFLERTEGSAIRRIGYERWLRNIAVALGNAPVTRENAAALAS
                     RSDDPSALVREHVAWARAEHSARTA"
     gene            complement(1850..2701)
                     /gene="panC"
                     /locus_tag="VIV63_00475"
     CDS             complement(1850..2701)
                     /gene="panC"
                     /locus_tag="VIV63_00475"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY6123095.1"
                     /translation="MQTVTTIAAVRAQVSQWHVAGERVVFVPTMGNLHAGHVSLIEAA
                     RRHGKRFVASIFVNPMQFGPNEDFAHYPRTPTQDAKMLADAGCDLMFMPEVGEIYPNG
                     AEQATRVEVPGISSILCGEFRPGHFEGVATVVAKLFNIVDADVAIFGEKDFQQLTVIR
                     RMAADLCLRVEIVGAPTVREADGLAMSSRNQYLDETQRTLAPTIYRQLQQAVAALKSG
                     VRDYSKIEATGRAALDGAGFRTDYFSVRDAKTLAPATPDTRDFVVLTASRLGKARLID
                     NIQFTAG"
     gene            complement(2713..3546)
                     /gene="panB"
                     /locus_tag="VIV63_00480"
     CDS             complement(2713..3546)
                     /gene="panB"
                     /locus_tag="VIV63_00480"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY6123096.1"
                     /translation="MYTHLEQRDSTRPPVSLGTLDKMKRAKEKIACITCYDASFAVLC
                     DNADTDLVLVGDSLGMVLQGHDTTVPVTMDDIVYHCKAVARGLHRPFLVADLPFASYP
                     TSDVALANSVRLMQEGGAEMVKLESGLGQLEIVEFLARHDIAVCAHLGLKPQSVHKTG
                     GFKVQGRDEVAAKRMLDSAKALESAGADIVLLECIPSELGKRITESLDVPVIGIGAGP
                     DTDGQILVLYDVLDITSGRKPRFVKNFMEGATSNVDALKRYVDAVKQKTYPAPEHEFH
                     A"
     gene            complement(3569..4255)
                     /locus_tag="VIV63_00485"
     CDS             complement(3569..4255)
                     /locus_tag="VIV63_00485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY6123097.1"
                     /translation="MSTPETETQPGAAVPAQRYIVVEGPIGVGKTSLAKRLAETLEAE
                     LVLEQDAQNPFLERFYRNQKSGALPAQLFFLFQRAQQLGTLKQQDLFAPRRVADYLFE
                     KDRLFAGLTLDAAEMALYEQVASRLAVDPPKPDLVVYLQAPVETLLQRIARRGISYES
                     GIDAAYLTRLNDAYARFFHEFDKAPLLIVNAASIDPVQNQADYDELVGAIRRMKKGRM
                     YYNPLRHSTI"
     gene            complement(4252..4746)
                     /gene="folK"
                     /locus_tag="VIV63_00490"
     CDS             complement(4252..4746)
                     /gene="folK"
                     /locus_tag="VIV63_00490"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY6123098.1"
                     /translation="MTHARWLPVYVGVGSNLADPQTQVRRAMQALDRLPDSRLIVISP
                     LYRTTPLGEVVQPAFVNAVAGLLSQSTPEDLLAALRSLETELGREPPRERWGPRVIDL
                     DLLVVGREERATETLTLPHAGIAERDFVLYPLADIAPDLDVPRLGRVASLRDRVANRG
                     IERL"
     gene            complement(4743..>5387)
                     /locus_tag="VIV63_00495"
     CDS             complement(4743..>5387)
                     /locus_tag="VIV63_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123099.1"
                     /translation="GARSLEVLQGHGLLAELLPTVASYLERHPDGAVSRLVRQGLANT
                     DQRVAAGKPVTPTFLFALLLYGPFAEIIEKQPQEKWHDIGAIVDAVDRAARGAQGRIS
                     IPKRFSLGVREMFAAQPRLEQPRGRRALRMLEQPRFRAGFDLLLLRADMGLASKELAE
                     WWTKVQEVSQTDRDRMADALAPPQRSTGGGGGGGGGRRGPRRRRGGRGRAPAAS"
BASE COUNT          884 a         1826 c         1774 g          903 t
ORIGIN      
        1 tgaccaacac gtgtccgtgg gtgcccttgt gcgattcgcg tgcgcggcgc ggcagcgccg
       61 aggcgagttc tccttcgtcg atgcggcgca tgagcggcgc gaactgcggc gtggccggcg
      121 ccaccacgcc gagatcgtcg agcagcacca cgcctgaatg ctcgggaccc gcgcccagga
      181 acagtcccga cttgagcgcc acgaaactca ccgtcacctc ggcgcgcacg gcggcctcgt
      241 gcaccgcgcc gctgtcggca tcgacaccgg acgggatgtc gagcgccatg accgggcgct
      301 tcgccgcgtt gatcgcgcgg atcacggcca gcgtttcggc gcgcggcgcg cccgcgagcc
      361 cgatgcctaa caacccgtcg acgatgacgt cgctgcccga gagcgcatcc gccacgaagg
      421 gatgcgcgct gccacccgcg gccagccact cttccattgc acggcgcgcg tcgccaccga
      481 gtttgtccgg cggcgcggcc gccagcacca gtgcttcgag tcccgcgagc tgcgccaggc
      541 gcgcgagcac atagccatcg ccaccgttgt tgcccggacc gcaaaccacc gcgacccgtc
      601 tcgcctgcgg ccagcgcgcg cgcaggacct tgagcgcgcc ctcggccgcc cgggtcatga
      661 gcgtgaaccc cggaacgcgg cgcttttcga tttcccacgc gtcgagcgcg cgcacctgcg
      721 cggcggaata gatggcggtc ggccggttca tggcggggaa ttatactgac gccgtgacgt
      781 tccccgcccc aaccagagac tccctgctcg acctcaaggg cgccctcgcg gcgctggccc
      841 gggagcgggg tttcgacgcg ctgggcgtca gtcacgtcca tctctcggcc gacgccgcgc
      901 acctcgaccg atggctcgac cacggcaaac acggcgaaat gggttacatg gccaaacatg
      961 gcacgcggcg caccgacccg tcggaactgg tgcccggcac ggtgcgcgtg ttgtcggcgc
     1021 gcatgaatta ctggccgccg gacgcgcgcg acgcctccga ggcgctcggc gacccggagt
     1081 cgggttacgt ctcccgctac gcgttgggcc gcgactatca caagttattg cgcaagtcgt
     1141 tgcaggggct ggccgacgat atcgttcaac gcatcgggcc gatgggttat cgagtgttcg
     1201 tcgactcggg gcctgtactc gagaaagcgc tggcgcgaaa tgcgggactc gggtggatag
     1261 gcaaacacac gaacctgatt gcgcgcgatg ccggttcgta tttcttcatc ggcgagatcc
     1321 tcaccgactt gccactgccg gtcgacgaag ccgccaccgc gcactgcggc acttgcagcg
     1381 cgtgtattcc cgcctgcccg accggcgcca tcaccgcgcc caaccagctc gacgcacggc
     1441 gttgcatctc gtacctgacc atcgagctcc atggcgcgat cccggtggag tttcgccgga
     1501 tgctcggcaa ccgaatctac ggctgcgacg actgccagat cgtgtgccca tggaacaagt
     1561 tcgcgcgtgc cgccgcgcat ccggatttca agacgcggca ccggctcgat tcatcgcggc
     1621 tcgacgaatt gtttgcctgg accgaggtcg agttcctcga acgtaccgaa ggctcggcga
     1681 ttcgccgcat cggttacgaa cgatggctgc gcaacatcgc ggtggcgctc ggcaacgcac
     1741 cggtcacgcg cgaaaacgca gcggcgctgg cctcgcgctc ggacgatccg tcagcactgg
     1801 tgcgcgaaca cgtggcgtgg gcgcgcgccg aacattcggc gcggacggcc tagcccgcgg
     1861 tgaactggat gttgtcgatg aggcgcgcct tgccgagacg cgacgccgtg agcaccacga
     1921 agtcgcgcgt gtcgggcgtc gccggggcca gcgtcttcgc gtcgcgcacg ctgaagtagt
     1981 cggtgcggaa acccgcgcca tcgagcgcgg cgcggcccgt ggcctcgatc ttcgagtagt
     2041 cacgcacgcc ggacttcagg gcggccaccg cctgctggag ctgccggtag atagtcggcg
     2101 ccagcgtgcg ctgcgtttca tcgagatact gattgcgcga gctcatcgcc agcccatcgg
     2161 cttcgcgcac cgtgggtgca ccgacgatct ccacgcgcaa gcacaggtcc gcggccatgc
     2221 ggcggatgac ggtgagctgc tggaagtcct tctcgccgaa gatcgccacg tccgcatcga
     2281 cgatgttgaa gagcttggcg acgacggtgg ccacgccttc gaaatgcccc gggcggaatt
     2341 cgccgcagag aatgctggag atgccgggca cctccacgcg cgtggcctgt tcggcgccgt
     2401 tcggatagat ctcgccgact tcgggcatga acatgagatc gcagccggca tcggccagca
     2461 tcttcgcgtc ctgcgtcggc gtgcgcggat agtgcgcgaa atcctcgttg ggcccgaact
     2521 gcatcgggtt cacgaagatg ctcgcgacga agcgtttacc gtgtcttcgc gcggcttcga
     2581 tgaggctcac gtgaccggcg tgcaggttgc ccatggtcgg cacgaacacc acgcgttcgc
     2641 cggcgacgtg ccattgcgac acctgcgcgc gtaccgccgc gatggtcgtg actgtctgca
     2701 tggatcccac gctcaagcgt ggaactcgtg ctcgggcgcc ggatacgttt tctgcttcac
     2761 cgcgtcgacg taacgcttca gcgcatcgac gttcgacgtg gcgccttcca tgaagttttt
     2821 cacgaagcgc ggcttgcggc ccgaggtgat gtcgagcacg tcatagagca cgaggatctg
     2881 gccgtcggtg tcggggcctg cgccgatgcc gatgaccggc acgtcgagcg actcggtgat
     2941 ccgcttgccg agctcggacg gaatgcattc gagcaacacg atatccgcgc ccgcggactc
     3001 gagcgccttg gcgctgtcga gcatgcgctt ggccgccact tcgtcgcggc cctgcacctt
     3061 gaaaccgccg gtcttgtgta ccgactgcgg cttgagcccg agatgcgcgc acacggcgat
     3121 gtcatgacgc gcgaggaatt cgacgatttc gagctggccg agcccgcttt cgagtttgac
     3181 catctccgca ccgccctcct gcatgaggcg gaccgaattc gccagcgcga catcggaagt
     3241 cggataactc gcgaacggca gatcggccac gaggaacggt cgatgcagac cgcgcgcgac
     3301 ggctttgcag tgatagacga tgtcgtccat ggtcacgggc accgtggtgt cgtgcccctg
     3361 cagcaccatg ccgagcgagt cgccgacgag cacgaggtcg gtgtccgcgt tgtcgcacaa
     3421 caccgcgaaa ctcgcgtcgt aacaggtgat gcacgcgatt ttttctttcg cgcgcttcat
     3481 cttgtcgagc gtgcccagcg aaaccggcgg acgcgtggaa tcgcgttgtt cgagatgcgt
     3541 atacaagttt aacccctcgt cagacgcgtc agattgtact gtggcgcaac gggttgtaat
     3601 acatacgacc cttcttcatg cggcggatgg cgcccaccag ttcgtcgtag tcggcctggt
     3661 tctgcaccgg atcgatgctg gccgcgttca cgatgagcaa cggcgccttg tcgaactcgt
     3721 ggaagaaacg cgcgtaggcg tcgttcagcc gggtgagata ggcggcgtcg atcccgcttt
     3781 cataggagat gccgcgcctc gcgatccgct gcagcaacgt ttcgacgggg gcctgcaggt
     3841 agaccaccag gtccggcttc ggcggatcca ccgcgagccg cgacgccacc tgctcgtaca
     3901 gcgccatctc ggccgcgtcg agcgtgaggc cggcgaacaa ccggtccttc tcgaatagat
     3961 agtcggcgac gcgccgcggc gcgaacaggt cctgctgctt gagcgtcccg agctgctgcg
     4021 cgcgctggaa caggaaaaaa agctgcgccg gcaaggcgcc cgatttctgg ttgcggtaga
     4081 aacgttcgag gaaaggattc tgcgcgtcct gctcgagcac cagttccgct tccagcgttt
     4141 ccgccagacg tttcgcgagg ctggtcttgc cgacgccgat cggaccttcg accacgatgt
     4201 agcgctgcgc cggaacggcc gcgcctggct gggtttcggt ctccggcgtg ctcacaggcg
     4261 ctcgatcccg cgattcgcga cgcggtcgcg cagcgatgca acgcggccga gacgcgggac
     4321 gtcgaggtcc ggcgcaatgt cggccagtgg atagagcacg aagtcgcgct cggcgatgcc
     4381 ggcgtgcggc agcgtcagcg tttcggtggc gcgctcttct cggccgacca ccagcaggtc
     4441 gaggtcgatg acccgcggac cccagcgctc gcgcggcggc tcgcgtccga gttcggtttc
     4501 gagcgatcgc aacgccgcca gcagatcttc cggcgtcgat tgcgacagca gtcccgccac
     4561 cgcgttgacg aacgccggct gcaccacttc acccaacggc gtcgtgcgat acagcggcga
     4621 gatcacgatg agacgactgt ccggcaggcg atcgagtgcc tgcattgcgc gccgcacctg
     4681 cgtttgcggg tcggcaagat tgctgcccac accgacgtag accggtagcc agcgcgcatg
     4741 cgtcatgaag ccgcgggtgc gcggcctcgt ccgccgcgac gccggcgggg tccgcgtcga
     4801 ccgccaccac caccaccgcc accaccggtg gaccgctgcg gcggcgccag tgcgtcggcc
     4861 atgcggtcgc ggtcggtctg cgaaacttcc tgaaccttcg tccaccactc cgcgagctct
     4921 ttggacgcca ggcccatgtc ggcgcgcagc agcagcagat cgaagccggc gcggaagcgt
     4981 ggctgctcga gcatgcgcaa ggcgcgacgg cctcgcggct gttcgagtct gggctgggca
     5041 gcaaacatct cgcgcacacc gagcgaaaac cgcttcggga tggagatgcg cccctgggcc
     5101 ccacgcgccg cgcgatcgac cgcatcgacg atggcgccga tgtcgtgcca cttctcctgc
     5161 ggctgtttct cgatgatctc ggcgaacggc ccgtagagca gcaatgcgaa caggaatgtc
     5221 ggcgtcacgg gtttgcccgc ggccacgcgc tggtcggtat tggccaggcc ctgccgcacg
     5281 agccgtgaca cggcgccgtc gggatgccgt tcgagataac tcgccacggt cggcagcaac
     5341 tccgccagga gtccatgacc ctgcagcact tcgaggctcc tggcgcc
//
LOCUS       DATDEU010000024         7118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1900433, whole genome shotgun sequence.
ACCESSION   DATDEU010000024 DATDEU010000000
VERSION     DATDEU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900433"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VIV63_00500"
     CDS             complement(<1..396)
                     /locus_tag="VIV63_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123100.1"
                     /translation="MKRAGMWLAVLAWPVFVVASPNGGPVGDFEAHTDVGSPKIAGYS
                     TWNAASQVYTLSAGGVNIWAKRDEFQFAYRRMKGDFIVQAQVEFQGKGVDPHRKAGIM
                     VRTSIADFDSAYVDGALHGDGLTSLQFRKA"
     gene            complement(520..1605)
                     /locus_tag="VIV63_00505"
     CDS             complement(520..1605)
                     /locus_tag="VIV63_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEY6123101.1"
                     /translation="MKPWKTSLTLCVMFAASGALAQNKYFADWPAGTAPAEVGKRVAE
                     RFLPTPHMEMPSHGPQALHYSHVGTWTGALQFAALTKDTELKTKLIDRFDPFFALTGS
                     RVPRTDHVDGTVFGALPLELYMQDARFRYRLMGLTFADAQWDRPLPDGLSNQTRWWID
                     DMYMITALQLAAYRATADTKYLERATQSMVAYLAKLQQPNGLFFHAPDVPFFWGRGDG
                     WVAVGMADLLRELPEKHKSRKVVLTAYKKMMATLLTHQAQNGMWRQLIDHPESWEETS
                     STAMFSYAFVTGVKNGWLAEDTYGPAARKAWIALVGYLTSDGLLREVCVGTGKKNDLQ
                     HYLNRPRVIGDTHGQAPMLWTATALLR"
     gene            complement(1602..2837)
                     /locus_tag="VIV63_00510"
     CDS             complement(1602..2837)
                     /locus_tag="VIV63_00510"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HEY6123102.1"
                     /translation="MKQTCLGAALVALTLFALNSGTSAANAAGATIAVTHELDAARPR
                     EVISVPFAQIASLMPEARMYHLVVRDSKGRVLPSQITNYEHDHRGAQYDDLVFSYDFA
                     AGEKRATFTLENLATASPPEAPCTYARLVPERFDDIAWENDRIAHRMYGLALNSPRAG
                     GERLRGSGIDVWAKRVTYPIVDRWYAKGHDQFHKDEEGEGLDLYSIGGSRGAGGTGVW
                     DGAKLWTSDNFVGAQVLSNGPRRAAFKLTYAPWDAGSAGQVIETKRFTVDCGSNLDAV
                     ESVFDFVPDNAVVGIGISEHPPAAGFPAAVLTKDPDGRWLSFWEENKDGGLGVAVILA
                     SDTTPAGFAHEDAPGGKGNANNLILAKGIDGVPLRYFTGAGWTKSGQFDGRAAWETYV
                     KDFSARVRKPLSINVSASK"
     gene            complement(2914..4203)
                     /locus_tag="VIV63_00515"
     CDS             complement(2914..4203)
                     /locus_tag="VIV63_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY6123103.1"
                     /translation="MQRKFSYIVIASALASLLGACGGGGGMAGGSSSSSGSSSSSGGS
                     SGGGTPQLPDPATAPALKTVIAARYGVVNYPVGAAIEPGSTTRTPDSALLIKHFSSIT
                     AENAMKPDSLWPNAAGSTTQPAATPDFTQADVLAAFAANNGMELRGHTLLWHQTAPSW
                     FFAGDRGNPANYRALVQQRLRTYIFAVVQHFPNVYAWDVVNEVASDTQNAANPYRTAS
                     PWFEAYSVGGMNGADYIRDAFDFANQARTSIGRSSLTMKLMLNDYNTELAGKRANVMQ
                     IVQGLVTAGVAIDGVGHQFHLQLNASVADVTAALAAVEAISPSLVNHVTELDVSIYAD
                     PGECYSQRTIPPCLANFGASPPQSVLSQQATLYRALFNAFNRPSVTSVSLWGLADDHT
                     WLNTFPVTRTNRPLLFDTVGNPKWAFWAVVDPALAIP"
     gene            complement(4228..5238)
                     /locus_tag="VIV63_00520"
     CDS             complement(4228..5238)
                     /locus_tag="VIV63_00520"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY6123104.1"
                     /translation="MIESAQILIRHGVDLKAFRDEIQPAGEPVILKGLVKDWPAVRAG
                     LESPRALGDYLRGFDRGKPVAVLEGPPSIRGHFFYREDMRGLNFERRPATIGATIERL
                     LAQLDDPNPPALYVESTPTAEHLPPFADANPHPLLPPTIAPRIWIGNTLVVQTHFDLS
                     SNIACVVGGRRRFTLFPPDQVANLYVGPIEFNISGPPISMVTLKSPDFARFPRFAEAL
                     KHSRSAELEPGDAIYIPYGWWHQVESLAPFNVLVNYWWNEARQTGSPFDTLLHAVLTL
                     RDLPPEQRAAWQALFEHYVFTAPDEGLAHLPREQRGMLGPPSAERAQAIRAILARAFS
                     RP"
     gene            5251..>7118
                     /locus_tag="VIV63_00525"
     CDS             5251..>7118
                     /locus_tag="VIV63_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEY6123105.1"
                     /translation="MLQRMAVRLAWTMIALSVTGLAQAEAPLMSGLFVDHAVLQRDRP
                     INVYGQAAPGEEVSVTLAGATAAAKADAQGAWSLTLAAMPAGGPHTLTARTASRVQTA
                     NDVLLGDVWLCSGQSNMEWPVRMSLDAYNEAALSANDRIRHVTIARATSAAPRQDFDQ
                     VLEWRIAGPATTEHFSATCYYFVRELQKTVNVPQGIISSNWGGSRIEPWMSERALREL
                     GGYQEPLELLGKLRTSKPMAFSHWGETWQKWWLGERAVTQGRQPWAIQGDATAWQRAP
                     TALGYWETWGVPALGRYDGMVWFRAHVKLDKAQSKQAAQLSLGLADDADVTWLNGRAV
                     GNGFGDEARLYALPPKLLKDGDNVVVVNVHDFWGSGGLHGAAENRAVVLADGTRIPLT
                     DWEYSVAPPGLWPPHAPWESLSGVNVLYNAMIAPLGKYGLRGVAWYQGEANAGLDDAR
                     RYQALLAAWMADWRRQFDAPLPFLIVQLANFGKLVDSPVDSGWAQLRDAQRRAVAADG
                     NAGLAVTIDIGNRDDIHPANKQEVGKRLARAARHVVYGEKISASGALPASATRAGGDV
                     TVTFTDFDGDLHVVGSKDPSGFEVCGATQDTCRFVRAQLSGGGKVLLSDAASASATR"
BASE COUNT         1183 a         2216 c         2434 g         1285 t
ORIGIN      
        1 cgccttgcgg aactgcagcg atgtgagccc gtcaccgtgc agcgcgccgt cgacataggc
       61 cgaatcgaaa tcggcgatcg aggtgcgcac catgatcccg gccttgcgat gcggatcgac
      121 gcccttgccc tggaactcca cctgcgcctg cacgatgaag tcgcccttca tgcggcgata
      181 cgcgaactgg aattcatcgc gcttcgccca gatgttcacg ccgcccgcac tgagcgtgta
      241 cacctgcgac gccgcgttcc aggtggagta cccggcgatc ttcggcgagc ccacgtccgt
      301 gtgcgcttcg aagtctccca ctggtccgcc attcggcgaa gccacgacga ataccggcca
      361 cgccagcact gccaaccaca ttcccgctct tttcacgatg cccccttccg gaaatggaaa
      421 tggggacatc cctcatttcc ttgtcacgat tcgccgattg ttacagggaa atgaggaatg
      481 tccccattcc cccgactttg gtctagctat cggcggatgt cagcgcaaca gcgcggtcgc
      541 tgtccacaac attggcgctt gtccatgcgt gtcaccgatg acgcgcggcc ggttcaggta
      601 atgctgcagg tcgttcttct tgcccgtgcc cacgcagact tctcgcagaa ggccatcgga
      661 cgtcaggtat ccgacgagcg cgatccacgc cttgcgcgcc gcgggaccgt aggtgtcttc
      721 ggccagccag ccgtttttca cgccggtcac gaacgcatac gagaacatcg ccgtgctcga
      781 cgtttcttcc cagctttccg gatgatcgat gagctggcgc cacatgccgt tctgcgcctg
      841 gtgcgtgagc agcgtggcca tcatcttctt ataggcagtc agcacgacct tgcgtgactt
      901 gtgtttctcg ggaagttcac gcaacagatc ggccatgccc acggccaccc agccatcgcc
      961 gcggccccag aagaaaggca cgtcgggcgc atggaagaac aggccgttcg gctgctgaag
     1021 cttggcgaga tacgcgacca tggattgcgt ggcgcgctcg agatacttcg tgtcggccgt
     1081 ggcgcgatac gcggcgagct gcagcgccgt gatcatgtac atgtcgtcta tccaccagcg
     1141 cgtctggttg gacagcccat ccggcagcgg ccggtcccac tgcgcgtcgg cgaaggtcag
     1201 gcccatgagg cgataccgga atcgcgcgtc ctgcatgtag agctcgagcg gcagcgcgcc
     1261 gaacaccgta ccgtccacgt gatcggtgcg cggcacgcgc gagccggtga gcgcgaagaa
     1321 gggatcgaaa cggtcgatga gctttgtctt gagctccgtg tccttggtga gtgcggcaaa
     1381 ctgcagcgcg ccggtccacg tgcccacgtg cgaatagtgc agagcctgcg gaccgtggct
     1441 gggcatttcc atgtgcggcg tcggcaggaa tcgttccgcc acgcgcttgc ccacttcggc
     1501 gggcgccgtg ccggccggcc agtcggcaaa gtacttgttc tgcgcgagcg cgcccgaggc
     1561 ggcgaacatc acgcagagag tcaatgaggt cttccatggc ttcatttgct ggcgctcacg
     1621 ttgatgctca ggggtttgcg cacgcgcgcg gaaaaatcct tcacatacgt ttcccaggcc
     1681 gcgcgtccgt cgaactgtcc gctcttcgtc cagcccgcgc ccgtgaagta acgcagcggt
     1741 acgccgtcga tccccttggc gaggatcagg ttgttagcgt tgcccttgcc gccgggcgca
     1801 tcctcgtgag cgaagcccgc gggcgtcgtg tcgctggcca ggatgacggc gacaccgagg
     1861 ccgccgtcct tgttctcttc ccagaagctc agccagcgtc catcgggatc cttggtgagc
     1921 acggcggccg ggaatccggc ggcgggcgga tgctcgctga tgccgatgcc cacgacggcg
     1981 ttgtcgggca cgaagtcgaa cacactctcg accgcatcga ggttgctccc gcagtcgacg
     2041 gtgaaacgct tggtctcgat gacctggccc gcgctgcccg catcccaggg tgcgtaggtg
     2101 agtttgaatg ccgcgcgccg cggaccgttc gacagcacct gcgctcccac gaagttgtcg
     2161 gaggtccaga gtttggcgcc atcccacacg ccggtgccgc ccgcgccgcg cgagccaccg
     2221 atgctgtaca ggtcgaggcc ttcgccttct tcgtccttgt ggaactggtc gtgacccttg
     2281 gcataccagc ggtcgacgat ggggtaggtc acgcgcttgg cccacacatc gatgccgctg
     2341 ccccgcagcc gctcgccgcc ggcgcgcggc gagttgagcg cgaggccgta catgcggtgc
     2401 gcgatgcggt cgttttccca cgcaatgtcg tcgaagcgct cgggcaccag gcgcgcgtag
     2461 gtgcagggtg cttcgggcgg tgatgcggta gccaggttct ccagcgtgaa tgtggcgcgc
     2521 ttttcgccgg ccgcgaaatc gtacgagaac accaggtcgt cgtactgggc gccgcgatgg
     2581 tcgtgctcgt agttagtgat ctgggaaggc agcacgcggc cttttgaatc gcgaaccacg
     2641 aggtggtaca tgcgcgcctc gggcatcagc gatgcaattt gcgcgaatgg gacggagatg
     2701 acttcacgcg gacgcgccgc gtccaattca tgagtcacgg caatcgtggc gccagcggcg
     2761 ttcgcggccg acgtcccgga attcaatgcg aaaagggtca gcgcgaccag ggccgcgccg
     2821 agacatgtct gcttcatttt tcccccacgg acccaacgag tccggctgat tcgcgctctg
     2881 gcgcgattgt cgcctattcg gtggcccgcc ggttcaagga atcgcgagcg cgggatcgac
     2941 taccgcccag aacgcccact tgggattgcc caccgtgtcg aacagcaacg ggcgattggt
     3001 gcgcgtcacc gggaatgtat tgagccaggt gtgatcgtcc gcgagacccc agaggctcac
     3061 agacgtcacg ctgggccggt tgaaagcatt gaacagcgcg cgatacaacg tggcctgctg
     3121 cgagagcacc gattgcggcg ggctcgcgcc gaagttggcg agacagggcg gaatggttcg
     3181 ttgtgagtaa cactcgccag gatcggcgta gatagagacg tcgagttcgg tcacgtggtt
     3241 gacgaggctg ggggagatcg cctccaccgc ggcgagcgcc gccgtgacgt ccgccacgga
     3301 cgcgttgagc tgcaaatgga actggtgacc gacgccgtcg atcgcaaccc cggcggtcac
     3361 cagtccctgc acgatctgca tgacattggc gcgtttgccc gcgagctcgg tgttgtagtc
     3421 gttcagcatc agcttcatgg tgaggctgga tctgccgatt gacgtgcgcg cctggttggc
     3481 gaaatcgaag gcgtcgcgga tgtaatcggc accgttcatg ccgccgacgc tgtaggcctc
     3541 gaaccagggg cttgcggtgc gatacgggtt ggcggcgttc tgcgtatcgc tggccacttc
     3601 gttgaccacg tcccaggcat acacattcgg gaagtgctgg accacggcga agatgtatgt
     3661 gcgcagccgc tgttgtacca gcgcccggta gttagcgggg ttgccgcggt cgccggcaaa
     3721 gaaccacgac ggcgcggtct ggtgccacaa cagcgtgtgg ccgcgcagtt ccatgccatt
     3781 gttcgcggcg aaagccgcca gcacgtcggc ctgcgtgaag tcgggtgtcg ctgccggctg
     3841 ggtggtgctg cccgcggcgt tcggccagag cgaatccggc ttcatcgcgt tttcggcggt
     3901 gatgctgctg aagtgtttga tcagcagagc ggagtccggc gttcgggtgg tcgaacccgg
     3961 ttcgatcgcc gcgcccaccg gataattgac gacgccgtag cgtgcggcga tgacggtctt
     4021 gagcgcgggc gccgtggccg gatccggtaa ttgcggcgtg ccgccgccgc tgctcccgcc
     4081 gctgctgctg ctactgccgc tactgctgct actgcccccg gccatgccac cgcctccgcc
     4141 gcatgcgccg aggagactcg cgagcgcact cgcgatgacg atgtacgaaa acttccgctg
     4201 catttttccc ccgctcgagt tctgaattta cggtcgcgaa aaggcccggg cgagaatggc
     4261 gcgaatcgcc tgcgcgcgct ccgcggacgg aggccccagc atgccgcgtt gctcgcgcgg
     4321 cagatgcgcc agcccttcat cgggcgcggt aaacacgtaa tgctcgaaaa gcgcctgcca
     4381 ggccgcgcgt tgctccggcg gcaggtcgcg cagcgtgagc acggcgtgca gcagggtgtc
     4441 gaacggggag ccggtctggc gcgcttcgtt ccaccagtag ttcaccagca cgttgaacgg
     4501 cgccagcgac tcgacctggt gccaccaacc atacggaatg tagatcgcgt cgccgggttc
     4561 gagttccgcg ctgcgtgaat gcttcagcgc ctcggcgaat cgcgggaatc gcgcgaagtc
     4621 cggagatttc agcgtcacca tgctgatcgg cgggccggag atgttgaact cgatgggccc
     4681 gacatacagg ttcgcgacct ggtccggcgg aaacagcgtg aagcgacgcc ggccgccgac
     4741 cacgcaggct atgttgctcg acaggtcgaa gtgcgtctgc acgacgagcg tgttgcctat
     4801 ccagatgcgc ggcgcgatcg tcggcggcaa cagcggatgt gggttcgcgt cggcgaacgg
     4861 cggcaggtgt tcggcggtcg gcgtggactc gacgtacagc gccggcggat tcggatcgtc
     4921 gagttgcgcc agcaatcgtt cgatggtcgc gccgatggtg gcggggcgcc gttcgaagtt
     4981 gagtccgcgc atgtcttcgc gatagaaaaa atgcccgcga atcgatggcg ggccctcgag
     5041 caccgccacg ggcttgccgc gatcgaagcc gcgcagatag tcaccgagcg cacgcggtga
     5101 ctcgagtccg gcgcgcaccg cgggccagtc tttcaccagg cctttgagta tcaccggttc
     5161 gcccgcgggc tggatctcgt cgcgaaatgc cttgagatcg acgccgtgcc ggatgagtat
     5221 ctgtgcgctt tcaatcatga gggtggcggg atgttacagc gaatggccgt gaggctggca
     5281 tggacgatga ttgccctgtc ggtgacaggc ctggcgcagg ccgaggcgcc gctgatgagc
     5341 gggctgttcg tcgatcacgc cgtgctccag cgcgaccggc ctatcaatgt gtacgggcag
     5401 gcggcgccgg gtgaggaggt cagcgtgact ctcgcgggtg ctacggccgc ggccaaggcc
     5461 gatgcgcagg gcgcctggtc gctgacactg gccgcgatgc ccgcgggcgg cccgcacacg
     5521 ctcacggcgc gaaccgcatc gcgcgtgcag acggcgaacg acgtgctgct cggcgacgtg
     5581 tggctctgct cgggccagtc gaacatggaa tggccggtgc gcatgtcgct cgacgcgtac
     5641 aacgaagctg cgctgtcggc caacgatcgc attcgtcacg tcaccatcgc gcgcgccacg
     5701 agcgcggcgc cgcgccagga tttcgaccag gtgctcgaat ggaggatcgc cgggcccgcc
     5761 accaccgaac atttctctgc cacctgttac tacttcgttc gtgaattgca gaagaccgtg
     5821 aacgtaccgc agggaatcat ctcgtcgaac tggggaggct cgcgcatcga gccgtggatg
     5881 agcgagcgcg cattacgcga actcggtgga taccaggagc cgctcgagtt gttaggaaag
     5941 cttcgcacca gcaaaccgat ggcattctcg cactggggtg aaacctggca gaaatggtgg
     6001 ctcggtgagc gtgcggtgac gcaaggcagg caaccgtggg cgattcaggg cgacgcgacc
     6061 gcgtggcagc gcgcgccgac ggcgttgggc tactgggaga cctggggagt ccccgcgctc
     6121 ggccgttacg acggcatggt gtggtttcgt gcgcacgtga agctcgacaa ggcgcagtcg
     6181 aaacaggcgg cgcagttgtc gctcggactt gcggacgatg cggatgtgac gtggctgaat
     6241 ggccgcgcgg tcggtaatgg attcggtgac gaggcgcgtt tgtacgcgct gccgccgaaa
     6301 ctgctcaagg acggcgacaa cgtcgttgtc gtgaacgtgc acgatttctg ggggagtggc
     6361 gggctgcatg gcgccgcgga gaatcgcgcc gtggtgctgg ccgacggcac gcgcattccg
     6421 ctcaccgact gggaatacag cgttgcgccg ccgggattgt ggccgccgca tgcgccgtgg
     6481 gagtccttgt ccggcgtcaa cgtgttgtac aacgcgatga tcgcgccgct cggcaagtac
     6541 gggctgcgcg gcgtggcctg gtaccagggc gaggccaacg cgggactcga cgatgcgcgc
     6601 cgttaccagg cgctgctcgc ggcgtggatg gcggactggc gccggcagtt cgacgcgccg
     6661 ctgccgttcc tgatcgtgca gctcgcgaat tttggcaagc tcgtcgactc gccggtagat
     6721 agtggctggg cgcagctgcg agatgcgcag cggcgcgccg tcgcggcgga tggcaacgcg
     6781 gggctggccg tgaccatcga catcggtaat cgcgacgaca tccacccggc gaacaagcag
     6841 gaggtcggca agcggctggc gcgggccgcg cggcacgtgg tgtatggcga gaagatctcg
     6901 gcgtcgggcg cgctgccggc gtcggcgacg cgggcgggtg gcgacgtcac ggtcacattc
     6961 acggatttcg atggcgacct gcacgtggtc ggctcgaagg atccgtccgg tttcgaggtg
     7021 tgtggcgcga cgcaggacac ctgccggttc gtgcgtgcgc agctttccgg tggcggcaag
     7081 gttttgttga gcgatgcggc gagtgcgagt gcgacccg
//
LOCUS       DATDEU010000025         6112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1608787, whole genome shotgun sequence.
ACCESSION   DATDEU010000025 DATDEU010000000
VERSION     DATDEU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608787"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="nadC"
                     /locus_tag="VIV63_00530"
     CDS             <1..754
                     /gene="nadC"
                     /locus_tag="VIV63_00530"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY6123106.1"
                     /translation="SQQVRAQIIAREPAVLCGTDWADATFKQLDPAIRLDWQVRDGGR
                     LEADLPVLKLSGPARPILTGERTALNFLQTLSATATAASRYVEAVAGTGCKILDTRKT
                     LPGLRLAQKYATLCGGAQNHRLGLYDMLLIKENHIIAAGSIGAAIATARQKSPGIPVE
                     VEVESLDEFDQALAAAADIIMLDELVLDDMREAVKRNRSKGAKAKLEASGSVTLATVR
                     EIASTGVDYISIGGITKHIQAVDLSMRFEFAA"
     gene            751..1707
                     /locus_tag="VIV63_00535"
     CDS             751..1707
                     /locus_tag="VIV63_00535"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY6123107.1"
                     /translation="MKSRLLFLLTGVLLTACVRHDESTLPGTLERDRVELVADANEAI
                     VSLPFAEGAAVKPGDVVAVQDRALSTADLDAARAQLAEAESRAEELKNGPRATTIRAA
                     AARRDTARVQRDDAVRERDRLLGLVARSLVSKSEADRQSAAADAANATLREAEADLRE
                     LQEGTRTEQLAQAREAASIARANLQSLETTSSRLEVRSPVAGTIDSLPFRVGEKPARG
                     ATVAVLLATTAPFARIHVPEPIRAKVKIGTPATLRVDGLERTFKGQVRFIASEAEFTP
                     YYSLTAADRSRLSFVAEVVFDDSDASLPSGVPVDVTLDLESP"
     gene            1704..2636
                     /locus_tag="VIV63_00540"
     CDS             1704..2636
                     /locus_tag="VIV63_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6123108.1"
                     /translation="MSGTAITARQLTRKFGEKVAVDHIDLDIPSGRIYGFLGPNGSGK
                     STTLRMLCGMLLPSDGHAEVFGLSVVKDAEAIRRRLGYMPQKFSLWEDLTTDENLRFI
                     AELYGLEGDVEARIAEQRATYNLEALRKQRAGTMSGGQKQRLALAAATLHSPELLLLD
                     EPTSAVDPQSRRDFWERLFQLAEAGATILVSTHYMDEAERCHGLAIIAEGRVVAEGTP
                     RALMAGVEANIFEIEGADGSEAPRLIRALPWVHGVTQLGIRLRVLANRSVTDAEAQLR
                     ALLQQNGIEAKVELTDASLEDVFVVATRPEKLGR"
     gene            2626..3759
                     /locus_tag="VIV63_00545"
     CDS             2626..3759
                     /locus_tag="VIV63_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6123109.1"
                     /translation="MAANKVHAAFRRLLAVATKEVRQLSRDRVTFGMIVGVPLMQIVL
                     FGYAINYDVRNLATVVQDEANSSMSREFIAQLKATQVVHVSYVTNSSDELAQLMREGR
                     ASMAVVIPHDFERRLQAETRPVVQILVDGSQPQLAVVAGGIAAMPLLTRHGQGVYSGG
                     KRMAPRRIEIRTLYNPEKRTAVQIVPALIGVILTMTMVLFTSGAIVRERERGNLELLI
                     ATPLSSLELMVGKLLPYVGIGLIQTTIILIVGAWLFSVPVVGSLVNLYAACLMFIAAT
                     LGLGLFVSTLAQTQFQAFQLAFLTLLPSILLSGFMFPFEGMPVAAQWIAQLLPLTHFN
                     VIIRGIMLRGADLPEAWLQLLKLAAFLTVMLMVAVVRFKKRLD"
     gene            3815..4609
                     /locus_tag="VIV63_00550"
     CDS             3815..4609
                     /locus_tag="VIV63_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6123110.1"
                     /translation="MTSNAVLTTLLLALCASPSLAKPPVWPTGKVAAVALTYDDALHS
                     QLDIAIPQLDAAGFKGTFFLDGDITPADMLRWREAQRSGHELGNHSLFHPCPRAMLPD
                     RKNYLTDNYDVDRMLGEIAAMNNVLFGIDGARTRTYSVPCSQMFVGGVDYTAALRRSG
                     LVKYARTGGDQYKSVIADWSRLDYFQVPSYGPVDKPDGAALIAYAARVRDAKGLGVLQ
                     FHGVGGDYLEVTSKAHQQLVTWLRDHPEIWVARFDELMDYASAQSR"
     gene            4639..5469
                     /locus_tag="VIV63_00555"
     CDS             4639..5469
                     /locus_tag="VIV63_00555"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY6123111.1"
                     /translation="MDDKAQAERAQRFRALHVSGTFVIANCWDGGTARILTSLGFPAL
                     ATSSGASAGTLGRLDGNVSIDEALSQARTICSSTSLPVSADLEKGFGDDPAAAAKAIA
                     LAAGTGLVGGSIEDASGRKDAPIFDIALATERVAAAVEAARKLPFPFTLTARSENYLH
                     GRPDLDDTIRRLQAFERAGADVLFAPGLPDLAAVRTVCAALSKPVNFMVGIRGKSFSV
                     AELADAGVRRISLATSLYRAAMTGLADAAREIRDSGTFGYLDDTMSSGDFASYVQANP
                     "
     gene            5466..5987
                     /locus_tag="VIV63_00560"
     CDS             5466..5987
                     /locus_tag="VIV63_00560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6123112.1"
                     /translation="MKFVECTAERHSAAILEIFNEAIANSTAIYEYELLKPDYMVKWF
                     DTKARGNYPVIGLETDAGELAGFASFGKFRDRPAYKYTIEHSVYVETRFRGQKLGSRL
                     LEAVMEAAVKQNYHVMMGGIDAANAVSIRLHERFGFTYCGTVAQAGFKFGRWLDLAFY
                     QRVLSGPLAPVEG"
BASE COUNT         1076 a         2161 c         1857 g         1018 t
ORIGIN      
        1 ctcacaacag gttcgcgcgc agatcattgc gcgcgaacct gcggttctct gcggcaccga
       61 ctgggccgat gccaccttca aacaactcga tcccgccatt cgtctcgatt ggcaggttcg
      121 cgacggcggc cgcctggagg ccgatctgcc ggttctcaag ctgtcaggcc ccgcgcgccc
      181 catcctcacg ggtgaacgaa cggcgctgaa cttcctgcag acgctttccg ccaccgccac
      241 ggcagcgagc cgatacgtgg aagccgtcgc gggcaccggc tgcaagattc tcgatacgcg
      301 caagaccctg cccggcctgc ggctggcgca gaaatacgcc acgctctgcg gcggcgcgca
      361 gaaccatcgc ctggggctct acgacatgct gctcatcaag gagaaccaca tcatcgcggc
      421 cggctcgatt ggcgcggcca tcgcgaccgc acgccagaag tctccaggca tcccggtcga
      481 agtcgaagtg gagtcactcg acgaattcga ccaggcgctc gccgccgctg ccgacatcat
      541 catgctcgac gaacttgtcc tcgacgacat gcgcgaggcc gtgaaacgca atcgctcgaa
      601 aggcgcgaaa gcgaaactcg aggcctccgg cagcgtgaca ctcgccaccg tgcgcgaaat
      661 cgcctcgacc ggcgtcgact acatttccat cggcggcatc accaagcaca tccaggcggt
      721 cgacctgtcg atgcgattcg agtttgccgc gtgaaatcgc gcctcctctt cctgctcacc
      781 ggcgtgttgc tcaccgcttg cgtgcgtcac gacgaaagca ccctgcccgg tacgctggaa
      841 cgcgatcgcg tggaactggt cgccgatgcg aacgaagcca tcgtctcgct gccgttcgcc
      901 gaaggcgcag cggtcaaacc cggcgatgtg gtcgcggtcc aggatcgggc gctgtcgacc
      961 gccgacctcg acgccgcccg cgcccagctc gccgaggccg aatcccgcgc cgaggaactc
     1021 aagaacggac cgcgcgccac aaccattcgc gctgccgccg cgcgccgcga cacggcgcgc
     1081 gtgcaacgtg acgatgctgt gcgcgaacgg gaccggctgc tcggcctcgt ggcgcgcagc
     1141 ctggtcagca agtcggaggc cgaccggcaa tccgcggcgg ccgacgccgc caacgcgacg
     1201 ctgcgcgaag ccgaggcgga tcttcgcgaa ctgcaggaag gcacccgcac cgaacaactc
     1261 gcgcaggcgc gcgaagccgc gagcatcgcg cgcgccaatc tgcagagcct cgaaaccacc
     1321 tcttcccgac tcgaggtgcg ctcgcccgtg gccggcacca tcgactcgct gccctttcgc
     1381 gtgggcgaaa aacccgcgcg cggcgccacc gtcgccgtcc tgctggcgac caccgcgccc
     1441 ttcgcgcgca tccacgtgcc ggagcccatc cgcgcgaagg tgaagatcgg cacgccggcc
     1501 acgttgcgcg tcgatggcct cgaacgcacg ttcaagggcc aggtgcgatt catcgccagt
     1561 gaggcggagt tcacgcctta ctactcgctg accgcggcgg accggtcgcg gttgtcgttc
     1621 gtcgccgaag tcgtgttcga cgactccgac gcttcgctgc cttcgggcgt gcccgtcgac
     1681 gtgacgctgg acctggaatc gccgtgagcg gcactgccat caccgcgcgc cagctcacgc
     1741 gaaagttcgg tgagaaggtc gcggtggacc acatcgacct cgacattccg tccggccgca
     1801 tctacggatt cctcggaccg aacggctcgg gcaaatccac gacgttgcga atgctctgcg
     1861 gcatgttgct gcccagcgat ggtcacgccg aggtgttcgg cctttcggtc gtcaaggatg
     1921 ccgaagccat ccggcggcgc ctcggttaca tgccgcagaa gttctcgctc tgggaggacc
     1981 tgacgaccga cgagaatctg cgattcatcg ccgagctcta cggactcgaa ggcgacgtcg
     2041 aggcgcgcat cgccgaacag cgcgccacct acaatctcga ggcactgcgc aagcaacgcg
     2101 ccggcaccat gagtggcggc cagaagcagc gcctggcgct ggctgcggcc acgctgcatt
     2161 cgcccgaact cctgctgctc gacgaaccga ccagcgccgt ggacccgcaa agccgccgcg
     2221 atttctggga gcggctgttc cagctcgccg aagcgggcgc gaccatcctg gtgtcgactc
     2281 actacatgga tgaagcggag cgttgccatg gcctggcgat catcgccgaa ggtcgcgtgg
     2341 tcgccgaagg cacgccgcgc gcgctcatgg cgggcgtcga ggccaacatc ttcgaaatcg
     2401 aaggcgccga cggcagcgaa gcgccgcgcc tcattcgcgc attgccgtgg gtgcacggcg
     2461 tgacccagct cggcatccga ctgcgcgtgc tggccaaccg cagcgtgaca gacgccgagg
     2521 cgcaactacg cgcgctgctg caacagaatg gcatcgaagc gaaagtcgag ctgaccgacg
     2581 ccagcctcga agacgtgttc gtggtcgcca cgaggcccga aaaacttggc cgctaacaag
     2641 gttcacgccg cgtttcgccg cctgctggcg gtcgccacca aggaagtgag gcagctttca
     2701 cgcgaccgcg tcaccttcgg catgatcgtc ggtgtgccgc tcatgcagat cgttctcttc
     2761 ggctacgcca tcaactacga cgtgcgcaat ctcgccaccg tggtgcagga cgaggcgaac
     2821 tcttcgatgt cgcgcgagtt catcgcgcag ctcaaggcca cccaggtcgt gcacgtgagc
     2881 tatgtgacga actcttcgga cgagctcgcg cagctcatgc gcgagggccg cgccagcatg
     2941 gcggtcgtga tcccccacga cttcgagcgc cgtctgcagg ccgaaacccg gccggtggtc
     3001 cagatcctgg tcgatggatc tcagccccaa ctcgccgtcg tggccggcgg catcgccgcg
     3061 atgcctctgc tcacgcggca cggccagggc gtgtattcgg gcggcaaacg catggcgccg
     3121 aggcgcatcg agatccgcac gctctacaac ccggaaaaac gcacggcggt gcagatcgtg
     3181 ccggcactca tcggcgtgat cctgaccatg accatggtgc tgttcacctc gggcgccatc
     3241 gtccgcgagc gcgaacgcgg caatctcgag ctgctcatcg ccacaccgct cagcagcctc
     3301 gagctcatgg tgggcaaact actgccctat gtcggcatcg gcctgatcca gacgaccatc
     3361 atcctgatcg tgggggcatg gctgttcagc gtcccggtag tgggaagtct cgtgaatctc
     3421 tacgccgcgt gtctcatgtt catcgccgcc acgctcggcc tcgggctctt cgtctccacg
     3481 ctggcgcaaa cgcagttcca ggcgttccag ctcgccttcc tcaccctgct gccatcgatc
     3541 ctgctgtcgg gcttcatgtt tccgttcgaa ggcatgcccg tggcggcgca gtggatcgcg
     3601 cagttgctgc cgctcacgca tttcaacgtg atcatccgcg gaatcatgct gcgtggcgcc
     3661 gacctgcccg aggcctggct gcaactcctc aaactcgccg ccttcctcac cgtgatgctc
     3721 atggtcgccg tcgtccgctt caagaagcgc ctcgattaat ggggacattc ctgatttgct
     3781 ggcaaaaggg gacgcgcctc ccccgggagt tcccgtgaca tcgaatgccg tcttgaccac
     3841 gctcctgtta gccctctgcg cgagtccatc gctcgcaaag cctcccgtat ggccgaccgg
     3901 caaggtcgcg gccgtggcgc tcacctacga cgatgccctg cactcccaac tcgacatcgc
     3961 catcccgcag ctcgatgccg cgggattcaa gggcaccttc ttcctcgacg gcgacatcac
     4021 gcccgccgac atgcttcgat ggcgcgaggc ccagcgctcg ggccacgaac tcggcaacca
     4081 ctcgctgttt catccgtgcc ctcgcgcgat gctgcccgac cgcaagaact accttaccga
     4141 caactacgac gtcgaccgca tgcttggcga gatcgccgcc atgaacaacg tgctgttcgg
     4201 catcgacggc gcacgcacgc gcacctattc cgtgccgtgc agccagatgt tcgtgggcgg
     4261 cgtcgactac accgcggccc tgcgccgctc cggactcgtg aaatacgcgc gcaccggcgg
     4321 cgaccaatac aagtccgtga tcgcggattg gtcccggctc gattacttcc aggtacccag
     4381 ctatggcccc gtggacaagc ccgacggcgc cgcgctcatt gcctacgcgg cccgcgtgcg
     4441 ggacgccaaa ggactcggcg tcctgcaatt ccatggcgtc ggtggcgact acctcgaggt
     4501 cacgagcaag gcgcaccagc agctcgtcac gtggctgcgc gaccatcccg aaatctgggt
     4561 cgcccgcttc gatgaactga tggattacgc gagcgcccag tctcgctaga cttgcggccc
     4621 cgaacttcgg agcccggcat ggacgacaaa gcccaggcag aacgcgcaca acgtttccgc
     4681 gcgctgcacg tcagcggcac cttcgtcatc gccaattgct gggatggcgg caccgcgcgc
     4741 atcctcacct cgctgggctt tccagcgctc gccacttcca gcggcgcctc cgccggcacg
     4801 cttggccgcc tcgacggcaa tgtgtcgatc gacgaagcct tgtcgcaggc gcgaaccatc
     4861 tgcagctcca cgtcgctgcc ggtgtccgcg gatctcgaaa agggtttcgg tgacgatccg
     4921 gccgctgcgg ccaaggccat cgcgctcgcg gccggcaccg gtcttgtcgg cggttccatc
     4981 gaagatgcat cgggccgcaa ggatgcgccg atcttcgaca tcgcccttgc caccgaacgc
     5041 gtcgccgctg ccgtcgaagc tgcacgcaag ctgcccttcc cgttcacgct caccgcgcgc
     5101 tcggagaact atctacacgg ccggcccgat ctcgacgaca ccatcaggcg gctccaggct
     5161 ttcgagcgcg ccggcgccga cgtgttgttc gcacccggcc tgcccgatct cgcggccgtg
     5221 cgcaccgtgt gtgcggcgct cagcaaaccc gtgaacttca tggtgggcat acgcgggaag
     5281 tcgttctcgg tggcggagct agccgacgcc ggcgtgcgcc gcatcagcct cgccacctcg
     5341 ttgtatcgcg ccgccatgac cggcctcgcc gacgccgcgc gagaaatccg cgacagcggc
     5401 actttcggat acctggacga caccatgtcg agcggcgact tcgcaagcta cgtgcaggcc
     5461 aacccatgaa attcgtcgag tgcacagcgg aacgacactc cgcggccatc ctcgaaatct
     5521 tcaacgaagc gatcgcgaac tcgaccgcga tctacgaata cgaactgctg aagccggatt
     5581 acatggtgaa gtggttcgac accaaggcgc gcggaaatta tccggtgatc ggcctcgaaa
     5641 ccgacgccgg cgaacttgcg ggattcgcaa gcttcggaaa gtttcgcgac cggcctgcct
     5701 acaaatacac catcgagcat tccgtctacg tcgagactcg ctttcgtggc cagaaactcg
     5761 gcagccgcct gctcgaagcc gtcatggaag ccgcggtcaa acagaactac cacgtcatga
     5821 tgggcggcat cgacgccgcg aatgccgtga gcatcaggct gcacgagcgt ttcggcttca
     5881 cctactgcgg caccgttgcg caggccggat tcaagttcgg gcgctggctc gatctcgcgt
     5941 tttatcagcg agtgctgagc gggccgctcg cgccggtcga gggctgaacg gctccccacg
     6001 ccggggtcct gtctgctcga accccgccgc aatcgtgcag cgcgctcaat aatgcacgca
     6061 gtatgcagtg tcctcggcgc gaccggcgcg gacgggccct gttcggcgtt cg
//
LOCUS       DATDEU010000026         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_295982, whole genome shotgun sequence.
ACCESSION   DATDEU010000026 DATDEU010000000
VERSION     DATDEU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295982"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VIV63_00565"
     CDS             complement(<1..247)
                     /locus_tag="VIV63_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123113.1"
                     /translation="MFGFGDRRTHTRTLELPRSTPAISPRDHRVIVLANRAPIRHDFD
                     TSGRVVATRSTGGLVTALEPLVETYSGTWVAHAAGTAD"
     gene            467..1792
                     /locus_tag="VIV63_00570"
     CDS             467..1792
                     /locus_tag="VIV63_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6123114.1"
                     /translation="MRLKTWLVAALGLGSLVVLIAVSMLASSRKAQDIYTQLDQLNNH
                     HRRVEENLRRLRSDVNLSGIFVRDYLLDVARERAPEYRDQIAEFRRTNMATLVELEKL
                     IAREDRIASLQAKLDEYWETFDPLFDWTPAEKILLSASFLRREVVPRREAALTIAREI
                     EELNNANLSAQRDEVERRHTAFRDDLHRLMWQTVLLGLGVAMVVVFRLRVLERRSDEA
                     EHQMRELSQQLVNTQEEERKNLSRELHDHVAQVLTGLRMELGRIERMSSSVAPMIAEC
                     KALVDDMFATVRNLALGLRPSMLDDFGLQAALEWHVRDFMARYPINVDLMIDGDFDRL
                     PDSHRTCVYRIVQEAMTNCVRHADARNIRISVATADGQLRLSVSDDGVGLDPAHRRKG
                     LGLRGIDERVKELHGTVTISPEAERGTTLAVRLPLPTPMMEVALARAAG"
     gene            1779..>2172
                     /locus_tag="VIV63_00575"
     CDS             1779..>2172
                     /locus_tag="VIV63_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6123115.1"
                     /translation="MLLADDHSIVRRGLRGLLEAAGLTVVAEAADGLEAVRLCEEHRP
                     DLLILDIGMPQLSGIEVAARAQKMERPPGVIILSVHGDESYIMRALAAGARAYLLKSA
                     TDEDLIPAVRAVASGKPFFSPAVAAVLVE"
BASE COUNT          379 a          667 c          747 g          379 t
ORIGIN      
        1 tgtcggcggt cccagcggcg tgtgccaccc atgttcctga ataggtttca accagcggtt
       61 cgagcgcggt gaccaggcct ccggtcgatc gtgtcgcgac cacgcggccg ctggtgtcga
      121 agtcgtgtcg aatcggcgca cggttggcga gcacgatcac acggtgatcg cgcggcgaaa
      181 tcgccggcgt cgatcgcggc agctcgagtg tgcgagtgtg tgtgcgccga tctccaaatc
      241 cgaacatgtg ccctccgagt gcgctcgatc gcgtgatcgg agtagacctc gacgggtggc
      301 gcgattccat ccagtgaatg gaggagaaag ccggcttttg cgggggagcc gaggagcgcg
      361 ctgcttggcg aagaccgagc actgtcgcgc tggctccccc gtttacagga tgccggttca
      421 ggcagggatg tcataggctg gcagcaggga aggagttcac cgacacatgc gattgaagac
      481 atggctggtg gcggcacttg gcctgggcag cctggtcgtg ttgatcgccg tgtcgatgct
      541 ggcctcgtcg cgcaaggccc aggacattta cacgcaactc gatcagctca acaaccatca
      601 ccgccgggtg gaggagaacc tgcggcggct gaggtcagac gtcaacctgt cgggcatctt
      661 cgtgcgcgat tacctgctcg acgtcgcccg cgagcgcgcg ccggaatacc gcgaccagat
      721 tgccgagttt cgccgcacca acatggccac gctggtcgag ctggagaagt tgatcgcgcg
      781 agaggatcgc atcgcgagcc tgcaggccaa gctcgatgaa tattgggaga cgttcgatcc
      841 gttgttcgac tggaccccgg ccgaaaaaat tctgctgagt gccagcttcc tcaggcggga
      901 ggtggttcca cggcgcgagg ccgcgctgac catcgcgcgg gaaatcgagg agctcaacaa
      961 tgcgaacctg tccgcgcagc gcgatgaagt cgagcggcgt catacggcgt ttcgcgacga
     1021 cctgcaccgc ttgatgtggc agacggtact gctcggcctc ggtgtcgcca tggtcgtggt
     1081 atttcgcctc cgcgtcctgg agcggcgctc cgacgaggcc gaacaccaga tgcgcgagct
     1141 gtcgcagcaa ctggtcaaca cgcaggaaga agaacgcaag aacctgtctc gcgagcttca
     1201 cgaccatgtc gcgcaggtgc tgaccgggct gcgcatggag ctcggccgca tcgagcgaat
     1261 gagctcttcg gtcgcgccga tgatcgccga atgcaaggcg ctggtcgacg acatgttcgc
     1321 gacggttcgc aacctcgcgc tcggcctgcg ccccagcatg ctggacgact tcgggctgca
     1381 agccgcgctc gaatggcacg tccgcgattt catggcgagg tatccgatca acgtcgacct
     1441 gatgatcgac ggcgatttcg atcgactgcc ggacagccac cgcacctgtg tttaccggat
     1501 tgtgcaggag gcgatgacca actgcgttcg ccacgcggac gccaggaata tccgcattag
     1561 cgtcgccacc gccgacggcc agcttaggct gtcggtcagc gacgacgggg tcggcctcga
     1621 tccggcacat cgccgcaagg gtctcggttt gcgcggcatc gacgagcgcg tcaaggagct
     1681 gcacggcacg gtgaccattt caccggaagc ggagcgcggc accacactgg cggtccggct
     1741 accgttgccg acgccgatga tggaggtggc gcttgcgcgt gctgctggct gacgatcaca
     1801 gcatcgtcag gcgcggcctg cgcgggctgc tggaagccgc cggcctgacg gtcgtggccg
     1861 aggccgcgga cggtctcgag gcggtgcgcc tgtgcgagga gcatcgtccg gatctgctga
     1921 tcctcgacat cggcatgccg caactgagcg gcatcgaggt cgcggcgcgt gcgcagaaga
     1981 tggagcgtcc gcccggcgtc atcatcctga gcgtgcatgg cgacgagtcg tacatcatgc
     2041 gcgcgttggc ggcaggggcc cgcgcgtatc ttctgaagag cgccaccgac gaggacctga
     2101 ttcccgcggt gcgcgccgtc gcgtcaggca agccgttttt cagtccggcg gtcgcggcgg
     2161 tgctggtcga gg
//
LOCUS       DATDEU010000027         4565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_442000, whole genome shotgun sequence.
ACCESSION   DATDEU010000027 DATDEU010000000
VERSION     DATDEU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442000"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3381)
                     /locus_tag="VIV63_00580"
     CDS             complement(<1..3381)
                     /locus_tag="VIV63_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123116.1"
                     /translation="MRFTRIVAAAALVLAPSVTLAQSFTGTIVGTIKDSSGAAIPHAT
                     VSTTSQQTGRQDSVTADIEGRYTSLPLPPGEYRVEATLQGFKSAVRSNVVVTIASTVV
                     IDFALEVGELTEAVEVSASTLSLETTSSTVGKLVDNRRIQELPLNTRNVYSLIFLTPG
                     VAGTIGNNYNSMSYSVNGARPTMMDTVIDGVTASFPTVNGFTGISVFPSVDAIQEFKV
                     MGANYPAEYGRSLGSVLNVVYKSGSNDFHGSAYEFFRDSAFDENNYFSEKAGTPLGDF
                     QRSQFGGVAGGPIRRGKTFFMTSYEGLRQDSASETTTTVPTLAQRNGDFSQTFAQNGQ
                     LIRIFDPFTTRPNPSGSGFIRDPFPNNVIPADRMDPVARQVLNYYPLPNQPGNSVTGA
                     QNYFATGTAKLNVDNIDARIDHNFSEKARSFFRYSYRKTFSSPAEFFPEDLTIAEGRV
                     NEQNLAHNAVIDYSRTLSNTTQMNARLGFARTLFLFDNQGLGFKPSSLGLPVSIDQNV
                     DREMFPRFGVSGMVSLGGNDHRYNAFMSYTAAASLTKSRGPHTLKGGFEGRMLRVNVW
                     EARSAGTFNFRANETQGPNPNTASSTAGYGFASLLLGFGQPNDVLIQNWKNVASNSFY
                     WAFYAQDDWRVNSRLTLNLGVRYDIDVPRTERYDRMNYFDPDAPSPLAQQVPGFPNLQ
                     GGVVFVGVDGNSRYQYNWDTNNIAPRLGASYQLNDKTVVRAGYSHMFGPSNQGAQGTV
                     GPFGFRTENLWVTSIDGITPYNLLWNPYPNGFVPSPGSSQGLLTQAGANLQAPLQDTP
                     SPWTIQYNVNVQRELPWGLFVEAAYVGTRGYDLSIAGEGGLSLNQLDPQYMALGSQLN
                     QQVPNPFYGIVNNGVLTSSTVSRGQLLRPYPQFTDVVPLYAAGAKSRYNALQLTGRKR
                     LSRGFMFEGSYTFAKAEEMGMNHQNSYDIDASMALASYDINHRFVLSYLYEIPVGRDR
                     RFFANAPTVVNAIIGNWQFNGITTFQAGTPLSITANNTAGLFGARTQPNTTGADPRLD
                     GPVEDRLNRYFDTSVYSQPAAFSFGNEPIFSPILRAHGVRNVDLSLFKTFDITARLRA
                     QFRVEALNAFNRVQFSAPNTSVTSSSFGVITGQANAPRQ"
     gene            3467..4225
                     /locus_tag="VIV63_00585"
     CDS             3467..4225
                     /locus_tag="VIV63_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HEY6123117.1"
                     /translation="MPELPRRWIVFIALFSMACGGARSAPPATAPPPIASPAAAAPPA
                     RDPDAIRWVRDAAEYRAAVMQVYRQATARVEAEAAARKAGTWAVVLDADETVISNLTY
                     QVERSRAGLAYSPESWREWVSRRAATPLPGAAAFLARVHDLGGRIAIVTNRRQSECED
                     TIAVFRLHALQYDAVLCRSDGGPSDKNPRFEAVAAGQTTAGAGPLAVVAFVGDNILDF
                     PQASQASARENAASLDAFGVRFFVIPNPMYGSWQ"
BASE COUNT          751 a         1412 c         1560 g          842 t
ORIGIN      
        1 ctgccgtggc gcgttggcct gcccggtgat gacgccgaac gaggaggacg tgacgctggt
       61 gttgggcgcg gagaactgca cccggttgaa cgcgttgagc gcttcgacac ggaactgtgc
      121 tctcagccga gccgtgatgt cgaaggtctt gaagagcgag agatcgacgt tgcggacgcc
      181 atgcgcgcgg aggatcggcg agaagatcgg ctcgttgccg aacgaaaacg ccgccggctg
      241 gctgtagacg ctcgtgtcga aatagcggtt gaggcgatcc tcgaccggac cgtccaaacg
      301 cgggtccgcg ccggtggtgt tgggctgcgt gcgcgcgccg aacagtcccg ccgtgttgtt
      361 cgccgtgatc gacagcggcg tccccgcctg gaacgtcgtg atcccattga actgccagtt
      421 gccgatgatc gcgttcacga ccgtcggcgc gttggcgaag aatcgccggt cgcggccgac
      481 ggggatttcg tagagatagc tgagcacgaa gcggtggttg atgtcgtacg acgccaacgc
      541 catgctcgcg tcgatgtcgt agctgttctg atggttcatg cccatctcct cggccttggc
      601 gaaggtatac gacccctcga acatgaaccc gcgcgagagt cgcttccgcc cggtgagctg
      661 caacgcgttg tagcgcgact tcgcgcccgc cgcgtagagc ggcacgacgt cggtgaactg
      721 cgggtagggc cgcagcagtt gcccgcggct caccgtcgac gacgtcagca cgccgttgtt
      781 gacgatgccg tagaaggggt tgggaacctg ctgattgagc tgtgagccga gcgccatgta
      841 ctgcggatcg agctggttca ggctgaggcc gccttcgccc gcaatcgaca agtcatagcc
      901 gcgcgtgccg acgtaggcgg cctcgacgaa gaggccccag ggcagctccc gctggacgtt
      961 gacgttgtac tgaatcgtcc acggcgacgg cgtgtcctgc agtggcgcct gcaggttggc
     1021 gcccgcctgc gtgagcagcc cctgcgacga tcccggcgag ggcacgaagc cgttgggata
     1081 cggattccat agcaggttgt acggcgtgat gccgtcgatc gaggtcaccc acaggttctc
     1141 ggtgcggaag ccgaacggcc cgaccgtgcc ctgcgcgccc tggttcgacg gcccgaacat
     1201 gtggctgtag ccggctcgca ccacggtctt gtcgttcagc tgataggagg caccgagacg
     1261 cggggcgatg ttgttggtgt cccagttgta ctgatagcgg ctgttgccgt cgacgccgac
     1321 gaacacgaca ccgccctgca ggttggggaa cccaggcact tgttgcgcca gcggcgacgg
     1381 cgcatccgga tcgaaatagt tcatccggtc gtagcgctcc gtgcgcggca cgtcgatgtc
     1441 gtagcgcacc ccgaggttca gcgtcagccg ggagttgacg cgccagtcgt cctgcgcgta
     1501 gaacgcccaa tagaagctgt tggacgccac gttcttccag ttctgaatca gcacgtcgtt
     1561 cggctgcccg aacccgagaa gcagcgacgc gaacccatag cctgccgtgc tgctcgcggt
     1621 gttcggattg ggtccctgcg tttcgttggc gcggaagttg aacgtgccgg cgctgcgcgc
     1681 ctcccacacg ttcacgcgca gcatgcgccc ctcgaagccg cctttcagcg tgtgcggccc
     1741 gcgcgatttc gtgaggctcg ccgccgcggt gtagctcatg aacgcgttgt agcgatggtc
     1801 gttgccgccg agcgacacca tcccgctgac gccgaagcgc ggaaacatct cgcgatcgac
     1861 gttctgatcg atcgacaccg gcagcccaag gctcgacggc ttgaacccga gcccctggtt
     1921 gtcgaagagg aacaacgtgc gcgcgaagcc gaggcgcgcg ttcatctgcg tcgtgttcga
     1981 gagtgtccgg ctgtagtcga tcaccgcgtt gtgcgcgagg ttctgctcgt tgacgcggcc
     2041 ttccgcgatg gtgaggtctt cagggaagaa ctccgccggc gagctgaagg tcttgcggta
     2101 ggagtagcgg aagaacgacc gcgccttctc ggagaagttg tggtcgatgc gcgcgtcgat
     2161 gttgtcgacg ttcagtttgg cggtgccggt cgcgaaatag ttctgcgccc cggtcacgga
     2221 attcccgggt tgattgggca gcggatagta gttcagcacc tgtcgcgcca ccggatccat
     2281 gcgatcggcc ggaatcacgt tgttcgggaa cggatcgcga atgaagccgc tgcccgacgg
     2341 gttcggccgc gtcgtgaacg gatcgaagat gcgaatcagc tggccgtttt gcgcgaacgt
     2401 ctgcgagaag tcgccgttgc gttgcgcgag cgtcggcacc gtcgtggtcg tttccgacgc
     2461 gctgtcctgg cgcagccctt cgtacgaggt catgaagaag gttttcccac gtcggatcgg
     2521 gccgcccgcg acgccgccga actggctgcg ctgaaagtcg ccgagcggcg tgcctgcctt
     2581 ctccgagaag tagttgttct cgtcgaacgc ggagtctcgg aagaactcgt aggcgctgcc
     2641 gtggaagtcg ttcgagcccg acttgtagac gacgttgagg acgctgccca gactgcggcc
     2701 gtattccgcc gggtagttcg cgcccatcac cttgaactcc tggatggcgt cgaccgaggg
     2761 gaacaccgag atgccggtga agccgttgac tgtcgggaac gacgccgtca ccccgtcgat
     2821 gacggtatcc atcatcgtcg gccgcgcgcc gttcacgctg tagctcatcg agttgtagtt
     2881 gttgccgatc gtgccggcga cccccggcgt caggaagatg agcgagtaga cgttgcgggt
     2941 gttgagcggc aactcctgga tgcgccggtt gtcgacgagt ttgccgacgg tgctcgacgt
     3001 ggtctccagt gacagcgtac tggcgctgac ttccactgct tccgtgagct cgcctacttc
     3061 cagcgcaaaa tcgatgacga ccgtggaggc gatcgtcacg acgacattcg agcgtacggc
     3121 ggatttgaag ccctgaaggg tcgcctcgac gcggtactcc ccaggcggta gcgggagcga
     3181 ggtgtagcgt ccctcgatat cggcggtgac cgaatcctgc cgtccggtct gctggctggt
     3241 ggtactgacg gtggcatgcg ggatggcggc gccgctggag tccttgatcg tgccgacgat
     3301 cgtgccggtg aacgactgag ccaacgtaac cgacggagcc agaacgagcg cggctgccgc
     3361 cacgatgcgg gtgaaacgca tgggacctcc tcggattgac gaggactata cgccggtcgg
     3421 cggtccgtgt gtagacgccc gccatccgtg acacgatgcc atcgtcatgc ccgagttgcc
     3481 tcgccgatgg atcgtcttca ttgctctctt ctccatggcg tgcgggggcg cgcggagtgc
     3541 tccgcccgcc acggcgcctc cccccattgc gtcgccagcc gccgcagcgc cgcccgcgcg
     3601 cgatccggac gcgatccgct gggtgcgaga tgccgccgag tatcgtgcag cggtgatgca
     3661 ggtctatcgt caggcgacgg cgcgggtgga ggccgaagcg gcggcgcgta aagcggggac
     3721 gtgggcagtc gtgctcgacg cagacgagac cgtgatctcg aacctcacgt atcaagtgga
     3781 gcggtcgcgt gcgggactgg cgtattcacc agagagctgg cgtgaatggg tgagccgtcg
     3841 agcggcgacg ccactgcccg gcgctgcggc cttcctcgcg cgcgtccacg accttggcgg
     3901 acgcatcgcg atcgtcacca accggcggca gtctgagtgc gaggacacca tcgcggtgtt
     3961 tcgcctgcac gcgctccagt acgatgcggt gctgtgccgc agcgacggcg gtccttccga
     4021 caagaatcca cgcttcgagg ctgttgctgc cggccaaact accgctggcg cgggtccgct
     4081 cgccgttgtc gccttcgtcg gtgacaacat cctcgacttc ccacaggcca gccaggcgtc
     4141 ggcgcgagag aacgcggcaa gcctcgatgc cttcggcgtg cgcttctttg tgatcccgaa
     4201 tcccatgtac ggcagctggc agtgaacatc tggttccgac gtgtgagtca tcggtgtggt
     4261 cctgggcctc gagtgggctt gatcagatct cggggacacc ttcgcaaagg taaacacggc
     4321 ggaccgggca gggccccctc actgacggta gccggggcag tgagattacg aggcacggaa
     4381 gtccacgacg cggcctgtcg ctcactgtct cccgcctcca gcgtacgacc gacgcgcgct
     4441 gaagcttgcc ggcgagggca ggtccttcgc gctggcgacg gtcgtgccta cgaccggaag
     4501 cacgccgcag accgtcggcg ccaagctccc ggtgatggcc acggccgaga tcgagatccg
     4561 tggtg
//
LOCUS       DATDEU010000028         3213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1171429, whole genome shotgun sequence.
ACCESSION   DATDEU010000028 DATDEU010000000
VERSION     DATDEU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171429"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1562)
                     /gene="glmU"
                     /locus_tag="VIV63_00590"
     CDS             complement(195..1562)
                     /gene="glmU"
                     /locus_tag="VIV63_00590"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY6123118.1"
                     /translation="MKLAVVILAAGQGKRMKSDLPKVLQPLAGVPLLGHVVSRARTLN
                     PSSMHVVYGHGGDAVRTALAKENLQWALQAEQLGTGHAVMQAMPNVADDELVLVLYGD
                     VPLIRAETLRQLIDAAGAKAMSLLTVLLENPTGYGRIVRNARGAIQKIVEQKDASKAQ
                     LKIREGNSGIMAVPAKLLRKWLGKLKNANSQGEYYLTDVIAMAVKDKVKVTPLIAPTV
                     AEVLGVNDKVQLAELEAMHRADRAKELMVAGATLVDPARIDIRGEVTLGRDVFIDANV
                     LLEGNVTLGDRVRIGPNTVLRDVTIGADSVIHPNSVLEQSELGAGCLIGPYARVRPGS
                     KLGAGVHVGNFVELKKTIIADGSKANHLTYLGDAVIGKGVNVGAGTITCNYDGVNKST
                     TTIEDGAFIGSGNMLVAPVRIGKNATTGAGSTITKDAPDGQLTLSRGKQITLEGWKRP
                     EKKPK"
     gene            complement(1615..1833)
                     /locus_tag="VIV63_00595"
     CDS             complement(1615..1833)
                     /locus_tag="VIV63_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123119.1"
                     /translation="MCPGNDNQVDWTAPATPSGEGVVPSLSRKPKPRELPQTKRNVMD
                     TLLGKNNLAAFDAGGNDPYNATGRQFRR"
     gene            complement(1931..2473)
                     /locus_tag="VIV63_00600"
     CDS             complement(1931..2473)
                     /locus_tag="VIV63_00600"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY6123120.1"
                     /translation="MPRYVALFRGINVGKAKRIAMADLRTLLARLGYTDVATLLNSGN
                     AVFTGDAEPAAKHSDRIRQAVLKKTGIDALVIVKSGKDIAGIIEGNELLAVAHDHSRL
                     LVAMTNDAKALSALRALANADWGRERIHIAKHAGYLWCANGILESKAAEALLKGLKGT
                     GTTRNWATLTKIRALMGDER"
     gene            complement(2477..2779)
                     /locus_tag="VIV63_00605"
     CDS             complement(2477..2779)
                     /locus_tag="VIV63_00605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY6123121.1"
                     /translation="MYGLIGKMTAVPGKRDELLWILLGGIGEMPGCLSYVVARDPGDA
                     DAIWITEVWDSESSHKASLSLSAVQSSIKTAMPLIARFNEHIRTEPVGGHGIKRAT"
     gene            complement(2767..>3213)
                     /locus_tag="VIV63_00610"
     CDS             complement(2767..>3213)
                     /locus_tag="VIV63_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123122.1"
                     /translation="GSMLMGFDEQGHCFMFANGNCSIYSHRPETCRTYDCRVFTAAGL
                     NAGPDKTEINERIAGWRFEYPSARDEREHRAVTAAANFIRQHVIRFPGGRVPSRPSEV
                     AVLAVKTYEVFLDPPSSDAEIAEAIITTSRDFDRQAHRQEKAECTD"
BASE COUNT          494 a         1007 c         1059 g          653 t
ORIGIN      
        1 ggcaccatga cgctctggct gttgtaacca ccgaccatca ccccgggcag cacactggcg
       61 atgtgaccgt tgtgcagaaa cgcggggatc tcatgcgcct gcagcagcgc aaccaccacg
      121 gccgcctcgg acgcgctccg aggattcaga atccgaacca gtgccatggc cttccgtgta
      181 gcgtcgctgc gcgactactt cggtttcttc tctggccgct tccagccttc gagagtgatc
      241 tgcttgccgc gcgacagcgt gagctgcccg tcgggcgcat ccttggtgat ggtgctgccg
      301 gcgccggtcg tcgcgttctt gccgatgcgc acgggcgcca ccagcatgtt gcccgagcca
      361 atgaaggcgc cgtcttcgat ggtggtggtg gatttgttca cgccgtcgta gttgcaggtg
      421 atggtgcccg cacccacgtt cacgcccttg ccgatgacgg cgtcgccgag gtacgtgagg
      481 tgattggcct tgctgccgtc ggcaatgatc gtcttcttga gctccacgaa attgccgacg
      541 tggacgccgg cgcccagctt cgagccgggg cgtacgcgcg catacgggcc gatgaggcag
      601 cccgcgccga gctcgctctg ctcgagcacg gagttcggat gaatgacgct atccgcgccg
      661 atggtcacat cgcgcagcac ggtattcggc ccgatacgca cacgatcgcc gagcgtgacg
      721 tttccctcga gcagcacgtt ggcgtcgatg aagacatcgc gacccagcgt gacttcgcca
      781 cggatgtcga ttcgcgcggg atccacgagc gttgcgcccg cgaccatgag ttccttcgcg
      841 cggtcggcac ggtgcatcgc ctcgagctcc gcgagctgca ccttgtcatt gacgccgagc
      901 acttcggcca cggtgggcgc gatgagcggc gtgaccttca ccttgtcttt caccgccatg
      961 gcgatgacgt cggtgaggta gtactcgccc tgcgagttgg cgttcttgag cttgccgagc
     1021 cacttgcgca gtagtttggc gggcacggcc atgatgccgc tgttgccttc gcgtatcttg
     1081 agctgtgcct tgctggcgtc cttctgctcg acgatcttct ggatggcgcc gcgcgcgttg
     1141 cgcacgatcc ggccgtaacc ggtggggttc tcgagcagca ccgtgagcag actcatggct
     1201 ttcgcacccg ccgcgtcgat gagctgacgc agggtttcgg cgcggatcag cggcacgtcg
     1261 ccgtacagca ccagcaccag ttcgtcgtcg gcgacgttgg gcatggcctg catgaccgca
     1321 tggccggtgc cgagctgttc ggcctgcaac gcccattgca gattctcttt cgcgagcgcg
     1381 gtacgcaccg catcgccgcc gtggccatac accacgtgca tggacgaggg gttcagcgtg
     1441 cgcgcgcggc taacaacatg cccgagcagc ggaacgccgg cgagcggttg caggaccttg
     1501 gggagatcgg acttcattcg cttgccttga ccggcagcga ggatcacaac ggcgagtttc
     1561 atgagcccgc gagtttaacg ggccatttcg ggaatggaca atcgacaggg ccgctcagcg
     1621 gcggaattgc cgacccgtcg cgttgtaggg atcgttgccg ccggcatcga aggccgcgag
     1681 gttgttcttg ccgagcagcg tgtccatgac attgcgcttg gtctggggaa gctcgcgcgg
     1741 cttgggcttg cgcgacaggc tcgggacgac accttcgccc gagggggtgg cgggagcagt
     1801 ccagtcgacc tggttgtcgt ttccggggca catgggactg aaatatacgc ccggattctt
     1861 tgcagtggta tgtccgtggt cggccttcaa aggccgctcg tcgggatggc cgccggtcgt
     1921 ccggcgctat ctaccgctca tcgcccatga gcgcacggat cttggtgagc gtcgcccagt
     1981 tgcgcgtggt gccggtgccc ttgagccctt tgagcagcgc ctcggccgcc ttgctctcga
     2041 gaattccgtt ggcgcaccac aggtatcccg cgtgtttggc gatgtggatt ctttctctgc
     2101 cccagtcggc gttcgcaagc gccctgaggg ccgacagcgc cttggcgtcg ttggtcatgg
     2161 cgacgagcaa tcgcgagtga tcgtgggcga ccgccagaag ttcgttccct tcgataatcc
     2221 ccgcgatgtc cttcccggat ttcacgatga ccagcgcatc gatgccggtc ttcttgagca
     2281 ccgcctggcg tatgcgatcc gagtgtttgg cggcgggctc ggcgtcaccc gtgaatacgg
     2341 cgttgccgct gttcagcagt gtcgcgacgt cggtgtagcc gagcctggcc agcagcgtgc
     2401 gcagatccgc catggcgatg cgcttggctt tgccgacgtt gatgccgcgg aacaatgcga
     2461 cgtagcgggg catgggtcag gtcgcgcgct ttatgccgtg tccgccgacg ggttcggtcc
     2521 ggatgtgttc gttgaacctg gcgatgagcg gcatggcggt cttgatcgaa ctctgcaccg
     2581 ccgacaacga caaagatgcc ttgtggctcg actcgctgtc ccacacctcg gtgatccaga
     2641 tcgcatcggc gtcgcccggg tcgcgcgcca ccacgtagct caggcagccg ggcatttcgc
     2701 cgatgcctcc gagcagaatc cacagcagct catcacgctt gccggggacg gcagtcatct
     2761 tgcctatcaa tccgtacatt cggctttctc ctgtcgatgg gcctgccggt cgaaatcgcg
     2821 gctggtggtg atgatggcct cggcgatctc cgcgtccgag gatggaggat cgagaaatac
     2881 ttcgtaggtc ttcacggcca gcacggctac ttcgctggga cgcgaaggca cccggccgcc
     2941 gggaaagcgg atgacgtgct ggcggatgaa atttgccgcg gccgtgacgg cgcggtgttc
     3001 acgctcgtcg cgcgcgctgg gatattcgaa acgccagccc gcgatccgct cgttgatttc
     3061 ggtcttgtcg ggcccggcgt tcaggcccgc ggcggtgaac acgcggcagt cgtaggtgcg
     3121 gcaggtttcc ggccgatgcg agtagatgga acagttgccg ttcgcgaaca tgaagcagtg
     3181 cccctgctcg tcgaagccca tgagcatgct gcc
//
LOCUS       DATDEU010000029         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_296188, whole genome shotgun sequence.
ACCESSION   DATDEU010000029 DATDEU010000000
VERSION     DATDEU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296188"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VIV63_00615"
     CDS             <1..407
                     /locus_tag="VIV63_00615"
                     /inference="COORDINATES: protein motif:HMM:NF037222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123123.1"
                     /translation="LNALPQQDLQMSVIASTVGEERVIGVRLTNPGKAPALATKLTLV
                     TPKGERILPALYSDNYVAVLPGEPRKIIIRYPAKLGDAAAIRVRGWNVRPASVRVSGG
                     GGQAYLPEPWQYPLVNTPAATLKSATVVVPKR"
     gene            441..929
                     /locus_tag="VIV63_00620"
     CDS             441..929
                     /locus_tag="VIV63_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123124.1"
                     /translation="MTPGNLVVRAIYLATAVFGPTLALVVAAFMLSGLYGNPAKHWMT
                     IASILLPALVFFVLPGARALRRLVASGTVLPPELVQERPVVAWVVSLVGAFGHFCFVI
                     SMSAAFEIALEPARGEDVGGPLAFAFMLALLAYLGALLCGELALVGDGESDAARARLD
                     FS"
     gene            1004..2257
                     /locus_tag="VIV63_00625"
     CDS             1004..2257
                     /locus_tag="VIV63_00625"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6123125.1"
                     /translation="MPAQEITRVSAEIDKLYRRESRRIFATLIRLLGDFERAEEALHD
                     AFRAAMEKWPAEGLPQNPVSWLISAGRFKSIDKQRREKRFDHLEDRPDVSESLTDHAP
                     AFDEREELEDDRLRLVFTCCHPAIAPDAQVALTLREVCGLATEEIAAAYLTPAPTIAQ
                     RIVRAKSKIRDANIPYQVPSREELPARLESVLRVVYLVFNEGYSASSGESATRADLSA
                     EAIRLGRLIVELAPEPEAQGLLALMLLHESRRETRATPAGELILLDEQDRSLWNRALI
                     DEGAALVRTALSTQAVGPYSVQAAIAAVHAESPTAAETDWNEIVGLYDLLLRITPSPI
                     VALNRTVAIAMRDGPEAGLAQLDQLLAEGSLDGYQLAHAARADFCRRLGRHGEARDAY
                     ARAIKLTAQGPARRFLERRLAALPT"
BASE COUNT          410 a          867 c          801 g          417 t
ORIGIN      
        1 cgctcaatgc gctgccgcag caggatcttc agatgagcgt catcgcttcg acggtcggcg
       61 aggaacgcgt gatcggtgtg cggctgacca accccgggaa ggcgcccgcg ctcgccacca
      121 agctcacgct ggtgacgccg aagggcgagc gcatcctgcc ggcgttgtac agcgacaact
      181 acgttgccgt actgccgggc gagccacgca aaatcatcat ccggtacccg gcgaaactcg
      241 gggacgcggc ggcgattcgc gtgcgtggat ggaacgtgcg tccggcgtcc gtgcgggtgt
      301 ccggtggcgg tgggcaggcc tatctaccgg agccgtggca gtaccctctg gtgaacacgc
      361 cggcggccac gctgaaatcc gccaccgtcg tagtgccgaa gcgctagcga cgtacggact
      421 gccgtgacag actggcgcgc atgactcccg gcaacctcgt cgtgcgcgcc atctacctcg
      481 cgacggcggt gtttgggccg acgctggcgc tggtagtcgc ggctttcatg ttgtccggcc
      541 tctacggaaa cccggcgaag cactggatga ccatcgcttc gatccttctc cccgcgctgg
      601 tcttcttcgt gcttcccggc gcccgcgccc tgcgccgatt ggtcgccagc ggcaccgtgc
      661 ttccgccaga actcgtgcag gagcgccccg tcgtggcctg ggtggtatcg ctcgtcgggg
      721 cctttggcca cttctgtttc gtgatctcga tgtccgccgc ctttgaaatt gcgctggagc
      781 ccgcgagggg cgaagacgtc ggcggcccgc tcgcgttcgc gttcatgctg gccctgctcg
      841 cctatttggg cgccctgctc tgcggtgaac tggcgctggt gggcgacggt gagagcgacg
      901 cggcacgcgc ccgcctcgat ttcagttaac cacccagccc actccgtcga tctgccggac
      961 tccaatctag gaaatggggt ctgaccccat ttatcctgcg gggatgcccg cacaggaaat
     1021 cacgcgcgtc agcgcggaga tcgacaaact ttatcgcagg gagtcgcgcc gcatcttcgc
     1081 gacgctgatc cggctgctcg gcgatttcga acgcgcggag gaagcgctgc acgatgcctt
     1141 ccgcgcggcc atggagaaat ggccggcgga gggtctgccc cagaacccgg tgtcgtggct
     1201 catttccgcg ggccgcttca agtccatcga caagcagcgc cgcgagaagc gtttcgatca
     1261 tctcgaagac cgccccgacg tcagcgagag cctcaccgac cacgcgcccg ccttcgatga
     1321 gcgcgaagaa ctcgaagacg accgtctgcg cctggtattc acgtgttgcc acccggccat
     1381 cgcacccgac gcgcaggtgg cgctgacgct gcgcgaagtc tgcggcctcg ccacggaaga
     1441 aatcgcggcg gcctatctca cgccggcgcc cacgatcgcg cagcgcatcg tgcgtgcgaa
     1501 gtcgaagatc cgcgacgcga acattcccta tcaggtgccg tcgcgcgaag aactgccggc
     1561 ccgcctcgaa agcgtgctgc gcgtcgtata cctggtgttc aacgaaggct actcggccag
     1621 cagcggcgag tcggcgacgc gcgccgacct gtcggctgaa gccatccggc tcgggcggct
     1681 catcgtggaa ctcgcccccg aacccgaagc acaaggattg ttggcgctga tgctgctgca
     1741 cgagtcgcgc cgcgagacgc gcgcgacgcc ggcaggcgag ctgatcctgc tggacgagca
     1801 ggatcgttcc ctctggaacc gcgcgctcat cgacgaaggc gccgcgctcg tgcgcaccgc
     1861 gttgtccacg caggcggtcg gcccgtattc ggtccaggca gcgatcgccg ccgtgcatgc
     1921 cgagtcgccc accgccgccg agaccgactg gaatgaaatc gtcgggctct acgacctgct
     1981 gttgcggatc acgccctcgc ccatcgtggc gctcaaccgg accgtggcca tcgccatgcg
     2041 tgacgggccc gaagcgggcc tggcgcaact cgaccagttg ctcgccgaag gctcgctcga
     2101 tggctaccag ctcgcgcatg cggcgcgagc ggacttctgc cggcgcctgg gccgccatgg
     2161 cgaagcgcgc gacgcctacg cgcgcgccat caagctcacc gcgcagggac cggcacggcg
     2221 attcctggaa cgacggctgg cggcattgcc cacctgacat ccgtcgattc aagcacttac
     2281 ggactgcgcg aaatttggcg gcgcgctgtc gaaatccgcg ccgcccgttc gaccaactct
     2341 caaccgaagg agattcgcgt gaagtacctc tgcctggttt acctctcgcc cgaacactgg
     2401 aattccgcga ccgatgaagc gtgcctggcc tgcggcaacg agttgcgcgc cagcggccac
     2461 ttcatcaccg gaacgccact gcatccgacg cacac
//
LOCUS       DATDEU010000030         6832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1900851, whole genome shotgun sequence.
ACCESSION   DATDEU010000030 DATDEU010000000
VERSION     DATDEU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900851"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1604
                     /locus_tag="VIV63_00630"
     CDS             12..1604
                     /locus_tag="VIV63_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEY6123126.1"
                     /translation="MAHRLAAGEDVTEINDLRGTAFVRRSHDFIEIDSTHIDLPGRLN
                     PPIDPYAMEPEIRKANEEAAAVEAGEAPPPAKEVVVKFARRVKSEDRERSVIRMPSFE
                     KVTADPVLYAHASRILHLEANPGNARALVQRHGDLDVWLNPPPIPLTTKDMDFVYELP
                     YARKPHPVYGNAEIPAYKMIKFSIAIQRGCFGGCTFCSITEHEGRIIQNRSEGSVLRE
                     IEKIRDEVPGFTGVISDLGGPTANMYRIACKSKEIESACRKPSCVYPGICPNLNTDHS
                     ALINLYRKARALPGVKKILIASGVRYDLAIESPEYVKELATHHVGGYLKIAPEAIGEG
                     PLSKMMKPGVGTYYKFKELFDKFSKLAGKEQYLIPYFIAAHPGTTDKDMLELALWLKQ
                     NGFRADQVQAFLPGPMATATAMYHTLKNPLRKVTRDSEEVVIPKGIKQRRLHKAFLRY
                     HDANNWPVLREALRKMGRADLIGSGKMQLVPGYQPAGTGDAAEGKRGGSANRPFKTQH
                     TGLPRVPSVGPTRKNRRDPRSN"
     gene            1647..2285
                     /locus_tag="VIV63_00635"
     CDS             1647..2285
                     /locus_tag="VIV63_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123127.1"
                     /translation="MKARVLLVLSLFAGMSVITGCGQSVYSVKIPLSETPEAVFADAP
                     HVAIEDKREDGERVTHVNASIFSCQRWYGDETYQPPKLVYLDRLIADRVPAATPVSIR
                     LDKFDTIEYCDNTADRGAAAAVAGATGGATGVPIILPATTRPGGDSVHIHLAGTINGV
                     PFDVKRAFDYDGLPYKFLEMPAANNTYRELLRKLMGEMADEIVAKLSLKPAS"
     gene            2382..2915
                     /locus_tag="VIV63_00640"
     CDS             2382..2915
                     /locus_tag="VIV63_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6123128.1"
                     /translation="MLTLFKSWVRPDAFQTVLEERSVRWYNACLRITRDADLAADAVQ
                     DALLKAWHRRDDFRGEAALDSWIHRIALNSALDLVRRRKTRSEEELDDDSFVAAPSES
                     PDSEYAKQALGKDLSVAMQRLTMMERQAFVLKHIEGWRLDEIAESLQVNVNNVKHALF
                     RAIRKLRVDLHVWRGES"
     gene            2912..3631
                     /locus_tag="VIV63_00645"
     CDS             2912..3631
                     /locus_tag="VIV63_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123129.1"
                     /translation="MITDDELILYYYRDGLDAAERARIGKMLSEHPELAQRMQGLVAH
                     LDAAAALPEVPVPAQTRQRWQAALERAAQTQDPRNVAAPRAGRGFFTEFRWPVAAATA
                     AVIAFIVIVRMPTPTLPDQTAEVAAPADSTAYERGLKLHLASTERQLAGLENTDPKER
                     ARLIETIVAQNRMYALAAERAGEPQLARVLRAFAPILESLEHEGGESSASIAQLNFEL
                     RIMQGRLGAGGSSTNNPSTTL"
     gene            3647..4561
                     /locus_tag="VIV63_00650"
     CDS             3647..4561
                     /locus_tag="VIV63_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6123130.1"
                     /translation="MRLFTTVMVALLMAGTAVAQAPKKTEKVERAPNADEELSLAALE
                     GLMGQSPERALPIIKKVLAGSQTRLVKQRALFVLSQIDSPEAREILAQTARSTDASMR
                     GEAIRNIGIGGDPKSLDALLAIYNAGDAQVKQEVLQAWMIADHKQAVYQVALNAKSEE
                     EATQAIRMLGAMGATEELRKLGERPNASRGLIDAYAISGDLASLRKIAEGTGERSVRL
                     EAVQKIGIIDGEAARTALREIYLRNTDAEFKDAALHGMLISGDDQGVLALYRAAKTSE
                     EKRALLRMLSMIDGDAALQAIDAALETK"
     gene            4561..5565
                     /locus_tag="VIV63_00655"
     CDS             4561..5565
                     /locus_tag="VIV63_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6123131.1"
                     /translation="MNTRTFRVALSFAALTVTSQAFAAELTLPRDGWTSWQVAAVEGA
                     PDLCCWSSWDDANPMRTACALDDNRGNSGSRDHTTTDAVRVYARVAGGKVERLRVFSA
                     SCPVKADTEIQPLGNVSEDDSARWLVKLFKSGADWIKHEDSRESLLTALALHRGDFAF
                     NELAAIARSDARTEAREKAVFWLAHLRGNAGAEVATSVMFNDQDADIRKHAAFSITQS
                     KSPRVASDLIRLGNTDKDGDVRAQAWFWLAQSGYASAEEAIFAAIRKDADEDVSERAI
                     FALSQLPDDRGTKALITVAEDKTLSGEQRKRALFWLAQSESEGAQKYLEKILVGKTAN
                     "
     gene            complement(5644..6717)
                     /locus_tag="VIV63_00660"
     CDS             complement(5644..6717)
                     /locus_tag="VIV63_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY6123132.1"
                     /translation="MSLRIALWMVTGLLFIAPSWGRTPAAEPPRGTVVVETFTSTVLR
                     ENRIGLKAERTIHVLLPPGYAKSRQRYPVVYFLHNAWWSARQAFEDGRLQRLTERGFA
                     DGVVQEFILVAADYTGPTTGSLYENSPVSGRWIDYTVDEVVPLIDRKYRTLARRESRA
                     VVGDFFGGRGALKFAMVKADVFSVAYAMHPVATGTGDIPWASLEIDWPRMHAAKSYEE
                     LGGLGRSQIFWAIHQAFAPNVSQPPCFCDFYTGMKDGKPSYDPDRVRVMQTEFLLDGT
                     LEAAAPALRTMRGLAFDWGRFDTTQAHVTSNRQFSRKLEDLGVEHEGEEYRGDPWNRT
                     WTEDGRFAARVLPFLQKHLMAGE"
BASE COUNT         1339 a         2207 c         2147 g         1139 t
ORIGIN      
        1 agatctgcga gatcgcgcat cgcctcgccg ccggcgagga cgtcaccgag atcaacgatc
       61 ttcgcggcac tgcgttcgtg cgccggtcgc acgacttcat agaaattgac tcgactcata
      121 ttgatttacc cgggcgtctg aatcctccca tcgatccgta tgcgatggag cccgagatcc
      181 gcaaggccaa cgaagaggcc gccgccgtcg aagccggcga ggcgccgccg cccgcaaaag
      241 aagtggtcgt caagttcgcg cgacgcgtga agagcgagga tcgcgagcgc agcgtgatcc
      301 gcatgccgag cttcgagaag gtgaccgccg accccgtgct ctacgcgcac gccagccgca
      361 tcctgcatct cgaagccaac cccggcaacg cgcgcgcact cgtccagcgt catggcgacc
      421 tcgacgtgtg gctgaacccg ccgccgattc ctctaactac gaaggacatg gacttcgtgt
      481 acgagctgcc gtatgcgcgc aagccgcatc cggtctacgg caatgccgag atccccgcgt
      541 acaagatgat caagttctcc atcgccatcc agcgtggatg tttcggcggt tgcacgttct
      601 gcagcatcac cgagcacgag ggccgcatca tccagaatcg cagcgaaggc agcgtgctgc
      661 gcgagatcga gaagattcgc gacgaagtgc caggcttcac gggcgtcatc agcgatttgg
      721 gcggccccac cgccaacatg taccgcatcg cctgtaaatc aaaagagatc gagagcgcct
      781 gccgcaagcc gagctgcgtc tatcccggta tctgcccgaa tctcaacacc gatcacagcg
      841 cgttgatcaa tctctatcgc aaggcgcgcg cgctgcccgg tgtgaagaag atcctgatcg
      901 cgagcggcgt gcgctacgac ctcgccatcg agagccccga gtacgtgaag gaactagcta
      961 cccaccacgt cggcggatat ctcaagatcg cgcccgaggc catcggcgaa gggccgctgt
     1021 ccaagatgat gaagcccggc gtcggcacgt actacaagtt caaggagctg ttcgacaagt
     1081 tcagcaagct ggccggcaaa gagcagtacc tcattccgta cttcatcgcc gcgcatcccg
     1141 gcaccaccga caaggacatg ctcgagctcg cgctgtggct caagcagaac ggattccgcg
     1201 cggaccaggt gcaggcgttc ctgccaggcc cgatggccac cgcgaccgcc atgtatcaca
     1261 cgctcaagaa tccgcttcgc aaggtgacgc gcgacagcga agaggtcgtg atcccgaagg
     1321 gcatcaagca gcgccggctg cacaaggcgt tcctgcgtta tcacgacgcg aacaactggc
     1381 ccgtgctgcg cgaggcgttg aggaaaatgg gccgcgccga tctcatcggg agcgggaaga
     1441 tgcagctcgt gccgggctat caacccgcgg gcacgggcga tgcggcggaa ggcaagcgtg
     1501 gcggaagcgc caaccggccg ttcaagaccc agcacacggg attgccgcgc gtaccttcgg
     1561 tgggacccac ccggaagaat cggcgcgacc cgcgctcgaa ctagctatca tcgttgcggt
     1621 tcggcgtccg ctttccaggg ggagcgatga aggcacgcgt tttgttggtt ctgtcactgt
     1681 tcgcggggat gtccgtcatc accggctgtg gccagtcggt ctacagcgtg aagatcccgc
     1741 tcagcgagac tcccgaggcc gttttcgccg acgctcccca cgtggcgatc gaagacaagc
     1801 gtgaggacgg cgaacgcgtc acgcacgtga acgcgagcat cttcagctgc cagcgctggt
     1861 atggcgacga aacgtatcag cctcccaagc tcgtctatct cgaccggctg atcgcggatc
     1921 gggttccggc tgcaacgccg gtcagcattc gtctcgacaa gttcgacacc atcgaatact
     1981 gcgacaacac ggcggatcgc ggcgccgccg cggccgtggc cggtgcgacc ggtggcgcga
     2041 ccggcgtacc catcatcctt cccgccacca cgaggccagg cggtgacagc gttcacatac
     2101 atctcgccgg caccatcaac ggagtgcctt tcgacgtgaa gcgcgcgttc gactacgacg
     2161 ggctgcccta caagttcctg gagatgccgg cggccaacaa cacctaccgc gagctgttgc
     2221 gcaaattgat gggtgagatg gcggacgaga tcgtcgcgaa gctttcactt aagcctgcgt
     2281 cctgagggcc cgcggttttc gtcatttgac cgcccaaacg gggcggcgca ggcctcgaaa
     2341 cacggtccct aaccttttcc cacctcgagc gtcttaggtt cgtgcttacc cttttcaagt
     2401 cctgggtccg cccggacgcc ttccagaccg ttctcgagga acgcagcgtg cgttggtaca
     2461 acgcctgcct gcgaatcacg cgcgatgcgg acctggcggc ggacgcggtg caggatgccc
     2521 tgctcaaggc ctggcatcgc cgcgacgatt tccgcggcga ggccgctctc gattcctgga
     2581 ttcaccgcat cgcgctgaac tccgcgctcg acctcgttcg ccgtcgcaag acacgctccg
     2641 aagaggaact cgacgacgac agcttcgtcg ccgcgccatc cgaatcaccc gacagcgagt
     2701 acgcgaaaca ggcgctgggc aaggatctct cggtcgcgat gcagcgcctg acgatgatgg
     2761 agcggcaggc cttcgtgctc aaacacatcg aaggctggcg actcgacgag atcgccgaaa
     2821 gcctgcaggt aaacgtcaac aacgtgaagc acgcgctgtt ccgcgcgatc cgcaagctgc
     2881 gcgtcgacct ccacgtctgg agaggcgaat catgatcacc gacgacgaac tcattctcta
     2941 ttactaccgc gatggtctcg atgccgccga acgcgcgcgc atcggcaaga tgctcagcga
     3001 gcatcccgag ctggcgcagc gcatgcaggg cctcgtcgcg catctcgatg ccgcggccgc
     3061 gttgccggaa gtccccgtgc ccgcgcaaac gcggcagcgt tggcaggcgg cgttggagcg
     3121 ggcggcgcag acccaagacc cgcgcaacgt cgccgcgcca cgcgcgggcc gcgggttctt
     3181 caccgagttt cgatggccgg ttgccgctgc aacggcggcg gtcatcgctt ttattgtgat
     3241 cgtccggatg ccgacgccga cgctgccgga tcaaaccgcg gaagttgccg cgccggcaga
     3301 ctcgaccgcc tacgagcggg gattgaagct gcatctcgcg agcaccgagc ggcagctcgc
     3361 cggtctcgag aacacagatc ccaaggaacg cgcgcgcctc atcgagacca tcgtcgcgca
     3421 gaaccgcatg tacgcgctcg ccgccgaacg tgccggtgag ccgcagctcg cgcgcgtgtt
     3481 gcgcgccttc gcgccgatcc tcgaaagcct cgagcacgag ggcggcgagt catccgcgag
     3541 catcgcgcag ctcaacttcg aattacgaat catgcagggc cggctcggtg ccggtgggtc
     3601 atccaccaac aacccgtcca caacgcttta gtcagaggaa tttctcatgc gtctattcac
     3661 cacagtcatg gtcgcgcttt tgatggccgg tacggccgtc gcgcaggcac ccaagaaaac
     3721 cgagaaggtc gagcgtgctc ccaacgccga cgaagagctc tcgctcgcgg cgctggaagg
     3781 cctgatgggg cagtcaccgg aacgcgcctt gcccatcatc aagaaggtgt tggccggttc
     3841 gcagaccagg ctcgtcaagc agcgggcgct gttcgtgctg agccagatcg attcgccgga
     3901 agcgcgggag atcctcgcgc agacagcccg ctcaaccgat gcctcgatgc ggggtgaagc
     3961 gattcgcaac atcggtatcg gcggcgatcc gaagtctctc gatgccttgc tggctatcta
     4021 caacgccggc gatgcccagg tgaagcagga agtgctgcag gcgtggatga tcgcggatca
     4081 caaacaagcc gtgtaccagg tggcactcaa cgccaagtcg gaggaggaag cgacccaggc
     4141 cattcgcatg ctcggcgcca tgggcgccac cgaggaactg cgcaagctcg gtgagcggcc
     4201 gaatgcctcg cgggggttga tcgacgcgta cgcgatcagc ggcgatctcg ccagcctgcg
     4261 aaagatcgct gagggtactg gtgaacgctc cgttcgactc gaagccgtgc agaagatcgg
     4321 aatcatcgat ggcgaagccg cgcgcaccgc gctgcgcgaa atctatctgc gcaacaccga
     4381 tgcggagttc aaggacgccg cgctgcatgg aatgttgatc tcgggtgacg atcagggtgt
     4441 gctcgcgctg tatcgcgccg cgaagaccag cgaagagaag cgggcgctgc tacgcatgct
     4501 gtcgatgatc gatggcgatg ccgctctgca ggcgatcgat gccgcactgg agacgaagtg
     4561 atgaatacca gaacgtttcg cgttgcattg tccttcgccg cgttaaccgt aacgtcgcaa
     4621 gctttcgctg ccgaactcac gctgccgcgt gacggctgga ccagctggca ggtggccgcc
     4681 gtcgagggcg cgcccgacct gtgctgctgg agcagttggg atgatgccaa cccgatgcgc
     4741 actgcctgcg ctctcgacga caaccgcggc aactccggct cgcgggatca cacgaccacc
     4801 gacgccgtgc gcgtgtatgc ccgcgtggcc ggcggcaagg tcgagcggct gcgcgtgttc
     4861 tcggcaagct gcccggtgaa ggccgacacg gaaattcaac cgctcggcaa cgtgagcgag
     4921 gacgacagcg cgcgatggct ggtcaaacta ttcaagagcg gtgcggactg gatcaagcat
     4981 gaagactcgc gagagagcct gctgaccgcg ctcgcgctgc atcgcggtga cttcgctttc
     5041 aacgagctgg cggccatcgc tcgtagtgat gcccgcaccg aagctcgcga gaaggccgtg
     5101 ttctggctcg cccatcttcg cggtaatgcg ggagccgagg ttgcgacatc cgtcatgttc
     5161 aacgaccagg atgcggatat ccgcaagcat gcggcgttct cgatcacgca gtccaagtcg
     5221 ccgcgggtgg cgtcagacct gattcggctg ggcaacaccg acaaggacgg cgacgttcgt
     5281 gcccaggcct ggttctggct cgcgcagagc ggctatgcga gtgcggagga ggccatcttt
     5341 gcggcaatca ggaaggacgc cgatgaagac gtcagtgagc gcgccatctt cgcgctgtcg
     5401 cagctcccgg acgatcgtgg gacgaaggcg ttgatcacgg tggcggaaga caagacttta
     5461 tcgggcgagc agcgaaagcg ggcgctgttc tggctggcgc aatccgagtc ggaaggggcg
     5521 cagaaatacc tcgagaagat cctggtggga aagaccgcga actgagcgct tccgccaatc
     5581 gatgggtcca gggcagccgc cagtcgcgtc gcaaaggcgc tggcggcggc catcttccgc
     5641 cgcttactcg ccggccatca aatgtttctg caggaacggc agcacgcgtg cggcaaacct
     5701 cccatcctcc gtccacgtac gattccacgg atctccgcga tactcttccc cctcatgctc
     5761 cacgcccaga tcctccagct tccggctgaa ctgacggttc gacgtgacat gcgcctgcgt
     5821 cgtatcgaac cttccccaat cgaacgcgag ccctcgcatc gtgcgcaacg ccggcgcggc
     5881 cgcctccaga gttccatcta acaagaactc cgtctgcatc acgcgtacac gatcggggtc
     5941 gtacgacggc ttcccgtcct tcatcccggt gtagaagtcg cagaagcagg gcggctgcga
     6001 cacattcggc gcgaacgcct gatggatagc ccagaagatc tgcgagcggc cgagaccgcc
     6061 caactcttca tatgatttcg ctgcatgcat gcgtggccag tcgatctcca gtgaggccca
     6121 cggaatgtcg cccgtacccg tcgccaccgg atgcatcgca tacgcgacgc tgaagacatc
     6181 cgccttcacc atggcgaact tgagcgcacc tcggccaccg aagaaatccc ccaccacggc
     6241 gcggctttcg cgccgcgcga gcgtgcggta cttgcggtcg atgagcggca cgacttcatc
     6301 gacggtgtag tcgatccacc ggccgctgac gggcgagttc tcgtagaggc tgccggtggt
     6361 ggggccggtg tagtcggcgg cgacgagaat gaactcctgc acgacgccat ccgcgaaccc
     6421 gcgctcggtc agccgctgca ggcggccatc ctcgaaggcc tggcgcgcgc tccaccacgc
     6481 gttgtgcagg aaatacacca cggggtatcg ctgtctggac ttggcgtagc cgggcggcag
     6541 cagcacgtgg atggtgcgct cggccttgag accaatgcgg ttctcgcgga gcacggtcga
     6601 ggtgaacgtt tcgacgacga cggtgccgcg cggcggttcg gcggcgggcg ttcgccccca
     6661 tgacggtgcg atgaacagca agccggtaac catccacaac gcgatcctca aagacatgtt
     6721 cggtgctccg ttcgagttat tgggagcacg aattctgcga accgggcacg tgctggtcgt
     6781 cctgactgat cggcgaggac cgaaagtgag ataagtcacc ggcacgtgac ag
//
LOCUS       DATDEU010000031         5834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1900990, whole genome shotgun sequence.
ACCESSION   DATDEU010000031 DATDEU010000000
VERSION     DATDEU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900990"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VIV63_00665"
     CDS             complement(<1..1230)
                     /locus_tag="VIV63_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY6123133.1"
                     /translation="MTTQKSSRGLSLALFLSVSAAAAAAPYDIPASVESVAKAQITAG
                     ALEAPIRFLASDALEGRGPATRGDELARLYLAAELQSLGYLPGGEKGGWEQTVDVVGT
                     TAQLPAVWSFQGKNGNVDLKLSDDYIAGSGVQTPNAAIADAGLVFVGYGIEAPEYKWD
                     DFKGVDVKGKVLVMLNSDPDWDPKLFAGPTRLYYGRWTYKYESAARHGAAGVIIVHTT
                     PSAGYPWQVVQSSWGGEQFELPAESEPRIQLKGWATEDAVRRLMKGAGQDLDKLVAAA
                     KSRSFKPVPIALKTSIRFDNKVSRVRTANVAGLLPGSDPKLKDEVVIYSAHHDHFGIG
                     DPDSTGDNIYNGAEDNASGCAQLLAIARAMAALPERPRRSVLMLFVAGEEQGLLGSKY
                     YSLHPTFAPGKIAANINY"
     gene            complement(1300..2673)
                     /locus_tag="VIV63_00670"
     CDS             complement(1300..2673)
                     /locus_tag="VIV63_00670"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HEY6123134.1"
                     /translation="MINLSAFKAYDIRGRIPDELNPELVYQVGRAFAAFVKPKRVAVG
                     RDIRLTSDQFATELIRGLTDAGVDVLDIGLCGTEGVYFATFAWKLDGGIMVTASHNPP
                     DYNGLKLVREQSKPIGSDTGLKDIAAIIQSGKLPPVAAVRGKVTKLETSDKYIEHLLG
                     YVNLENMRKLKVVVDAGNGGAGLAVDKLEPHLPFEFIKVRHEPDGDFPQGVPNPMLEE
                     NRGPTLKALKESRADVGIAWDGDYDRCFFYDSKGSFIEGYYIVGLLAEAFLAKQPGAR
                     IVYDPRLTWNTIDTVKRFGGVPVMSKSGHAFIKQKMREVDGAYGGEMSAHHYFRDFSY
                     CDSGMIPWLLVLERVCESGKSLDELVAERIKAFPASGEINRKLPDARKVLVEAEAKFG
                     KGAIIDYTDGLSVDHPEWRFNVRASNTEPLVRLNVESRGNEALMREKTAELLAFMDGM
                     GAIKANH"
     gene            complement(2674..3810)
                     /locus_tag="VIV63_00675"
     CDS             complement(2674..3810)
                     /locus_tag="VIV63_00675"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY6123135.1"
                     /translation="MKLVPVILSGGAGTRLWPLSRELLPKQLLALTGERTMIQDTAAR
                     LAGFPDATTPLVVCNEAHRFLVAEQLREMGMPPSGILLEPVGRNTAPAIALAAHAARQ
                     LAGDDAIMLVLPADHVLRDLAAFQSSVRLAVPAAAAGKLVAFGIVARTPETGYGYIRR
                     GAGGGAVEPIAPVFPIEQFVEKPNEARAKEFIASGDYFWNSGMFMFGAARFLEELAAH
                     APDIAEVCARASATAVREKDFTRVDEAVFAACRSDSVDYAVMEKTRDAVMVPLDAGWN
                     DVGSWAALHEVLPADERGNVMRGDVLLEDSDGCYVHADSRLVAALGLQDMVIVETADA
                     VLVAPKSKVQDVKKLVARIKAAGRMEHVSHAAPVPIVVRPKGKN"
     gene            3860..4957
                     /gene="wecB"
                     /locus_tag="VIV63_00680"
     CDS             3860..4957
                     /gene="wecB"
                     /locus_tag="VIV63_00680"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY6123136.1"
                     /translation="MKSIHLIAAARPNFMKIAPLYHALAKEPWCRPHIVHTGQHYDHN
                     MSDAFFQDLRLPKPHFHLEVGSGSHAQQTGNVMIAYEAVATREKPDWIIVVGDVNSTA
                     ACAMVGAKLCIPVVHLEAGLRSRDRTMPEEINRLVTDAISDVLWTPSPDADENLLAEG
                     VAAERIDRIGNIMIDSYEMMREVIAADDSVKRLALTAGKFGVVTLHRPSNVDSRETLG
                     TLVRVLLQASEMLPLVFAVHPRTRKKLQDYGLEGQITGARNIKVIEPLGYVQFMNLVS
                     NSAAVITDSGGVQEETTYLGIPCFTLRENTERPITVTLGSNQLVQPQDLLARLAAGLE
                     VRGRSGQRPDLWDGKSAERAVARLKSRALGE"
     gene            complement(4964..>5834)
                     /locus_tag="VIV63_00685"
     CDS             complement(4964..>5834)
                     /locus_tag="VIV63_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY6123137.1"
                     /translation="KEGAAIEDFMRPDRVVVGADNPRVTELLRALYEPFTRSREKVIV
                     MDVKSAELTKYAANAMLATKISFMNELANLAERFGADIEKVRVGIGSDARIGYHFIYP
                     GLGYGGSCFPKDVQALVRSARGVNYEPQLLNSVEAVNKAQKEVLPGKILAHYGGNIAG
                     KTFAIWGLAFKPGTDDMREAPSRILIEALWKAGAKVRAYDPAAGKEAKRIWGERADLT
                     LTARAREALDGADALVIVTEWKEFRSPDFDHLKKALKAPVIFDGRNLYDPVSMKKQGF
                     SYYAIGRGERAKP"
BASE COUNT          995 a         1892 c         1896 g         1051 t
ORIGIN      
        1 atagttgatg ttggcggcga tcttgccggg ggcgaacgtc gggtgcagcg aatagtactt
       61 gctgcctaac aacccctgct cttctccggc gacgaacaac atgagcaccg aacggcgggg
      121 acgctcgggc agcgccgcca tggcgcgcgc gatggccagc agctgcgcgc agcccgacgc
      181 gttgtcttcg gcgccgttgt agatgttgtc gccggtcgag tcggggtctc cgatgccgaa
      241 gtgatcgtgg tgcgcagagt agatgaccac ctcgtccttg agcttcgggt cgctgcccgg
      301 cagcaggccg gcgacgttcg cggtgcgtac gcgcgaaacc ttgttgtcga acctgatgga
      361 ggtcttgagc gcaatgggca cgggcttgaa cgaacggctc ttcgcggcgg cgacgagctt
      421 gtcgagatcc tggcccgcgc ctttcatgag gcggcgcacg gcgtcttcgg tcgcccaacc
      481 cttgagctgg atgcgcggct cgctttcggc cggcagttcg aactgctcac caccccagga
      541 cgactgcacc acctgccacg ggtaaccggc cgacggcgtg gtgtgcacga tgatcacgcc
      601 cgcggcgcca tggcgcgcgg cgctttcgta cttgtaggtc cagcggccgt agtaaaggcg
      661 cgtcggacct gcaaacaact tcggatccca gtccggatcg ctgttgagca tgacgaggac
      721 cttgcccttc acgtcgacgc ccttgaaatc gtcccatttg tattcgggcg cttcgatgcc
      781 gtaaccgacg aacacgaggc cggcgtcggc gatggcggcg ttcggtgtct gcacgcccga
      841 gcccgcgatg tagtcatcgg acaatttcag atcgacgttt ccgttcttgc cctggaacga
      901 ccacacggcg gggagctgcg cggtggtgcc aacgacatcg acggtctgct cccagccacc
      961 cttctcgcca ccgggtagat agcccagcga ctggagctcc gcggcgaggt acagccgcgc
     1021 cagctcgtcg ccgcgcgtgg cggggccgcg gccttcgagc gcgtccgagg cgagaaagcg
     1081 gatcggggct tcgagagccc ccgcggtgat ctgcgccttg gcgacgcttt cgacactcgc
     1141 gggaatgtcg tagggagcgg cggcggccgc ggcggaaacg gagaggaaca acgcgagcga
     1201 cagcccgcgc gaagatttct gggtagtcat tgaagccctc tggcaggaat aggaatcgag
     1261 gtgcggcccc atttgctagg aaatggggag tgtccccatt tagtggttgg ccttgatcgc
     1321 gcccattccg tccatgaaag cgagcagctc ggcggttttc tcgcgcatca gcgcttcgtt
     1381 gccgcgcgat tcgacgttga gccgcaccag gggctcggtg ttcgaagcac ggacgttgaa
     1441 gcgccactcg gggtgatcga cggaaagccc atcggtgtaa tcgatgatgg cgcccttgcc
     1501 gaacttcgcc tccgcctcga ccagcacctt cctggcgtcc ggcagcttgc ggttgatctc
     1561 accactcgcc gggaacgcct tgattcgttc ggcgacgagt tcgtcgagcg acttgcccga
     1621 ctcgcatacg cgctcgagca ccagcaacca ggggatcatg ccgctgtcgc agtacgagaa
     1681 atcgcggaaa tagtggtgcg cgctcatctc gccgccatac gcgccatcca cttcgcgcat
     1741 cttctgcttg atgaacgcgt gccccgactt gctcatgacg ggaacgccgc cgaatcgttt
     1801 caccgtgtcg atggtgttcc acgtgaggcg tgggtcgtag acgatgcgcg cgccaggttg
     1861 tttggcgagg aacgcctcgg ccagcaggcc gacgatgtaa taaccctcga tgaatgaacc
     1921 cttggagtcg tagaagaagc agcggtcgta gtcgccgtcc caggcgatgc cgacgtcggc
     1981 gcgcgattcc ttgagcgcct tgagcgtcgg cccgcggttt tcttcgagca tggggttggg
     2041 cacgccttgc gggaaatcgc catccggctc gtgccgcacc ttgatgaact cgaacggcag
     2101 gtgcggttcg agcttgtcca cggcgaggcc cgcgccgccg ttgccggcgt cgaccaccac
     2161 cttgagcttg cgcatgttct cgagattgac gtagccgagc aggtgctcga tgtacttgtc
     2221 cgacgtctcg agcttcgtga ccttgccgcg gaccgcggcc accggcggca gcttgccgct
     2281 ctggatgatc gccgcgatgt ccttgaggcc ggtgtccgag ccaatgggtt tgctctgctc
     2341 acgcaccagc ttgagaccgt tgtagtccgg tggattgtgg ctggccgtga ccatgatgcc
     2401 gccgtcgagc ttccaggcga aggtcgcaaa gtacacgccc tcggtgccgc acaaaccgat
     2461 gtccagcacg tcgacgcccg cgtcagtgag gccgcggatg agctcggtgg cgaactggtc
     2521 gctggtgaga cggatgtcgc gccccaccgc cacgcgcttc ggcttcacga aggcggcgaa
     2581 ggcacggccc acctggtaga ccagctcggg gttgagttcg tcgggaatac ggccgcggat
     2641 gtcgtaggcc ttgaacgcgg agagattgat caatcagttc tttcctttcg gcctaactac
     2701 gatgggcacg ggcgcggcat gcgacacgtg ttccatgcgc ccggccgcct tgatgcgggc
     2761 gacgagcttc ttgacgtcct ggaccttgga cttgggcgcc accagcaccg cgtcggcggt
     2821 ctcgacgatg accatgtcct gcagacccag ggccgccacg aggcggctgt cggcgtgcac
     2881 gtaacagcca tccgagtctt ccagcaggac gtcgccgcgc atcacgttgc cacgttcgtc
     2941 ggccggcagc acttcgtgca gcgcggccca ggaacccaca tcgttccagc ccgcgtcgag
     3001 cggcaccatc acggcgtcgc gcgttttttc catgacggcg tagtccaccg agtcgctgcg
     3061 gcaggccgcg aacaccgctt catcgacgcg cgtgaagtct ttctcgcgca ccgcggtggc
     3121 gctggcgcgc gcgcagacct cggcgatgtc gggcgcgtgg gcagcgagtt cttcgaggaa
     3181 ccgcgcggcg ccgaacatga acatgccgga gttccagaag tagtcgcccg aggcgatgaa
     3241 ttccttcgcg cgcgcttcgt tgggcttttc cacaaattgt tcgatgggaa aaacaggggc
     3301 gatgggctca accgccccgc cacccgcgcc gcggcggatg tagccgtagc cggtttcggg
     3361 agtgcgcgcc acgatgccga aggcaacgag cttgcccgcc gcggccgccg gcaccgccag
     3421 gcggaccgag gactggaagg cagcgaggtc gcgcaatacg tgatcggcgg gcaacaccag
     3481 catgatggcg tcgtcaccgg cgagttgtcg cgccgcgtgc gcggcgagtg cgatggccgg
     3541 cgcggtgttg cggcccaccg gctctaacaa gatgcccgag ggcggcatgc ccatctcgcg
     3601 caattgttcg gcgaccagga accggtgcgc ttcgttgcag acaaccaaag gagtagtggc
     3661 atccggaaac cccgcgagcc gcgcggcggt gtcctggatc atggttcgct cgccggtcag
     3721 cgccagcagc tgcttgggaa gcaactcgcg cgacaagggc cagaggcgcg tgcccgcacc
     3781 cccggagaga atcaccggta ccagcttcat gggtacaaat cgtactagct ttgcggcctt
     3841 cgtgtaggct cgcgaaccca tgaaaagcat ccatttgatc gcggccgctc gcccgaattt
     3901 catgaagatc gcgccgctct accacgcgct ggcgaaagag ccgtggtgcc gcccgcacat
     3961 agtccatacc ggccagcatt acgaccacaa catgtcggat gcgtttttcc aggacctgcg
     4021 cctgccgaag ccgcatttcc acctcgaagt aggcagcggc tcacacgcgc agcagaccgg
     4081 caacgtgatg atcgcctacg aggccgtggc cactcgcgaa aagcccgatt ggatcatcgt
     4141 ggtgggcgac gtcaattcga ccgcggcctg cgccatggtg ggcgccaagt tgtgtatccc
     4201 ggtggtccat ctcgaagccg gccttcgcag ccgcgaccgg accatgcccg aggagatcaa
     4261 ccggctcgtc accgacgcga tctccgatgt gttgtggact ccgtccccgg acgccgatga
     4321 aaacctgctg gccgaaggcg tcgccgcgga acgcatcgac cgcatcggca acatcatgat
     4381 cgacagttac gaaatgatgc gtgaggtcat cgccgcggac gacagcgtca aacgcctcgc
     4441 gctgaccgcc ggaaaattcg gcgtcgtcac actgcaccgg ccgtcgaacg tggactcacg
     4501 cgaaaccctg ggcacgctcg tgcgcgtgct gctgcaggct tccgagatgc tgccgctggt
     4561 gttcgcggta catccgcgca cccgcaagaa gctccaggat tacgggctcg aggggcaaat
     4621 caccggcgcg cggaacatca aggtcatcga gccgctcggc tacgtgcagt tcatgaacct
     4681 ggtcagcaac tcagccgcag tgatcaccga ttccggcgga gtccaggaag agactaccta
     4741 cctgggcatc ccgtgtttca cgctgcgcga gaacaccgag cggcccatca ccgtgacgct
     4801 gggttccaac cagctcgtgc agccgcagga tctgctcgcg cgcctcgcgg ccgggctcga
     4861 ggtgcgcggc cgcagcgggc agcgccccga cctctgggac ggcaagagcg ccgaacgcgc
     4921 cgtggcgcga ctgaagtcgc gcgcgctcgg cgagtagttc gccctacggc ttggcgcgct
     4981 cgccgcgccc gatcgcgtag taactgaagc cctgcttctt catcgacacg gggtcgtaca
     5041 gattgcggcc gtcgaagatg accggcgcct tgagcgcctt cttcaggtgg tcgaagtcgg
     5101 ggctgcggaa ttccttccac tccgtgacga tgacgagcgc atcggcgcca tcgagcgctt
     5161 cccgcgcgcg tgccgtcagc gtgagatcgg cccgctcgcc ccagatgcgc ttcgcttcct
     5221 tgccggcggc agggtcgtag gcgcgaacct tggccccggc cttccacaac gcttcgatga
     5281 ggatgcggct cggcgcctcg cgcatgtcgt cggtcccggg cttgaacgcc agtccccaga
     5341 tcgcgaaagt cttgccggcg atgttgccgc cgtagtgagc caggattttt cccggcagca
     5401 cttccttctg ggccttgttc acggcctcga cggagttgag cagctgcggc tcgtaattca
     5461 cgccgcgcgc cgaacgcacc agcgcctgca cgtccttggg gaaacaggaa ccgccgtaac
     5521 ccagacccgg gtagatgaag tggtagccga tgcgcgcatc ggaaccgatg cccacgcgga
     5581 ccttctcgat gtcggcgccg aatctttcgg cgaggttcgc cagctcgttc atgaaactga
     5641 tcttggtcgc cagcatggcg ttggcggcgt actttgttag ctcggcggac ttcacgtcca
     5701 tcacgatgac tttttcacgg ctgcgcgtga acggctcgta tagcgcgcgc aggagttccg
     5761 tgacgcgtgg attgtccgca ccgacgacca cgcggtccgg ccgcatgaag tcctcgatgg
     5821 ccgcgccttc cttg
//
LOCUS       DATDEU010000032         4141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_296765, whole genome shotgun sequence.
ACCESSION   DATDEU010000032 DATDEU010000000
VERSION     DATDEU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296765"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VIV63_00690"
     CDS             <1..391
                     /locus_tag="VIV63_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6123138.1"
                     /translation="MERTRLGAMIRAGVDDMEMARGVGIPVSRLFTTVFLLGATLAGI
                     GGVLGGPILNAYPGLDTDMLPLALIVVILGGAGSLLGAFVGSFIVGFIYNFGIALVPD
                     LAYFVLFLPMVLVLVFLPQGLFGRVQI"
     gene            388..1464
                     /locus_tag="VIV63_00695"
     CDS             388..1464
                     /locus_tag="VIV63_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6123139.1"
                     /translation="MTLRTVVALVVCALALLVLPWIIGNEFYVNMATQVLIYALFALS
                     INMMLGYGGMVSLGHAGYLGIAGYACILAVTAGYDHLTSAIFAIAVSTAAAAFFGLLS
                     LRAPGLGFIMITLALGQIVWGIAYRANDLTGGDNGIRYPARPMPFGFDLRDAPSFYYF
                     TLAVFVVALFCIWRFSRSPFGASLMGARDQPRRMRMLGYNVWLIQWLTFVMAGFWASI
                     AGVLYVYYNLFLSPHAISLQQSAEILLMAILGGASTLTGPIVGAAIITLVKNVISTYV
                     ERWNTLLGLIFVVVIMFMPYGLVPGCAQLWRRLRIRGKTPKAASSPAAMRAHGASEDA
                     RDRAGDTRPEAGSLARDTASEPAP"
     gene            1461..2204
                     /locus_tag="VIV63_00700"
     CDS             1461..2204
                     /locus_tag="VIV63_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6123140.1"
                     /translation="MTFPALEVSHLKKRFGGLPATRDVSLSVMPGERRLIIGPNGAGK
                     TTLFNLITGDLAADAGSIRLFGQELRGMPTQRRVHLGLARTYQILTLFPRERLIHNVV
                     LALLGLDRRRWNPWSILTGNRDLYKNAHEALERVGLGDSAERIVVETSYGERRRLELA
                     MALAQKPKLLLLDEPLAGLSQGERQTVRTLLGTIPRDVTIVMIEHDMDTALGFAERIT
                     VLHYGEVIVEGTRAEVVADPRTREVYLGD"
     gene            2194..2913
                     /locus_tag="VIV63_00705"
     CDS             2194..2913
                     /locus_tag="VIV63_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6123141.1"
                     /translation="MATEALAISDIDTYYGDSHVLHGVSFSLQPGRLLGLLGRNGAGK
                     STCMSTIMGFLKPRRGSISLYGEPVAGLAPDVIARKGICLVPQGRRMFRTLTVRENLM
                     VAAQSQKKDNGAGWSIGRVFQTFPRLAERHAQVAGSLSGGEQQMLAIGRALMGNPRVL
                     LMDEPSEGLAPQLVAEVGRTIAQLKAEGQSIVLVEQNIKLTLDLADDVVIINTGRVVF
                     RGPASGIKLDDAIVSQHLGVF"
     gene            3028..4095
                     /locus_tag="VIV63_00710"
     CDS             3028..4095
                     /locus_tag="VIV63_00710"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6123142.1"
                     /translation="MATQWNKYEATAARKHGKPGREVRPKSTTIDIHSHVAIPQAAAY
                     VKPHLDMSTIALAHFASPETKALSQKQEEDIRARITGYDERLADLDTMGIDLQLVMPP
                     PNQCFYTVPLDISVQASRLVNDGLAEYVSRKPDRFVALGTVPLIHGGEAANELERCVK
                     SLGMKGVEILTNVAGRELSEPDFAPFWQKAEELDALVVIHPNGFTDAKRFTRFYFNNV
                     IGNPLETTIALHYLIFDGVLERHPRLKILAVHGGGYLPAYSGRIDHAWGARSDSHGDL
                     PQPPTSYLKKIYFDTVVFTPYQLAELVRQFGTDRIIMGTDYPFDMADFDPIGHVASVE
                     HFDPTTIAAIAGGNARKLLGI"
BASE COUNT          680 a         1409 c         1299 g          753 t
ORIGIN      
        1 gatggagcgc acccggctgg gggcgatgat ccgcgcagga gtcgacgaca tggagatggc
       61 gcgcggcgtc ggcatcccgg tgtcgcggct gttcaccacc gtcttcctgc tcggggccac
      121 gctcgcgggg atcggcggcg tgctgggcgg gccgatcctc aacgcctatc ccggcctcga
      181 taccgacatg ctgccgctcg cgctgatcgt ggtgatcctg ggcggcgccg gaagcctgct
      241 cggcgccttc gtcggcagct tcatcgtcgg cttcatctat aatttcggca tcgcgctggt
      301 gcccgatctc gcctatttcg tgctcttcct gccgatggtg ctggtgctgg tgttcctgcc
      361 gcagggattg ttcgggcggg tgcagatatg acgctgcgca ccgtcgtcgc gcttgtcgtc
      421 tgcgcgctgg cgctcttggt cctgccgtgg atcatcggca acgaattcta tgtcaacatg
      481 gcgacccagg tgctgatcta tgcgctgttt gcgctgtcga tcaacatgat gctcggctat
      541 ggcggcatgg tctcgctcgg tcacgccggc tacctcggca tcgccggcta cgcctgcatc
      601 ctcgccgtca ccgcgggtta cgaccaccta acgtcggcga tcttcgcgat cgcggtgtcg
      661 accgcggcgg cggcgttctt cgggctgctg tcgctgcgcg cgccgggtct cggcttcatc
      721 atgatcacgc tcgcgctcgg ccagatcgtc tggggcatcg cctatcgggc caacgacctc
      781 accggcggcg acaacggcat ccgctaccct gcacggccga tgccgttcgg tttcgacctc
      841 cgcgacgcac ctagtttcta ttatttcacg ctcgccgtct ttgtcgtcgc cctgttctgc
      901 atctggcggt tctcgcgctc cccgttcggc gcgagcctga tgggggcgcg cgaccagcca
      961 cggcgcatgc gcatgctcgg ttacaatgtc tggctgatcc aatggctcac gttcgtcatg
     1021 gccgggttct gggcgtcgat cgcgggcgtc ctctacgtct attacaatct gttcctcagc
     1081 ccgcatgcga tctcgctgca acagtcagcc gagatcctgc tgatggcgat cctcggcggc
     1141 gccagcacgc tcaccggccc gatcgtcggc gccgccatca tcacgctggt gaagaacgtg
     1201 atctcgacct atgtcgagcg ttggaacacg ctgctcggcc tgatcttcgt ggtcgtgatc
     1261 atgttcatgc cctacgggtt ggtccccggc tgcgcgcagc tgtggcggcg attgcgaatc
     1321 cggggcaaga cgccaaaggc ggcgagcagc ccggctgcga tgcgcgcgca tggcgcgtcg
     1381 gaagacgcgc gtgaccgcgc tggtgacact cgccctgaag cgggctcgtt ggcgcgcgac
     1441 accgcctcgg agcccgcgcc atgacgttcc ccgcgctcga agtctcgcat ctcaagaagc
     1501 gcttcggcgg actgcccgcc acccgggacg tgtcgctgtc ggtcatgccg ggcgagcggc
     1561 gcctgatcat cggcccgaac ggcgccggca agaccacgct gttcaacctg atcaccggcg
     1621 atctcgccgc cgatgccggt tcgatccggc tgttcggcca ggagctgcgg gggatgccga
     1681 cccagcggcg cgtacacctc ggcttggcgc gcacctatca gattctcacg ctgtttccgc
     1741 gcgagcggct catccacaac gtggtgctgg cgctgctcgg gctcgaccgg cggcgctgga
     1801 acccgtggtc gatcctgacc ggcaaccgcg atctctacaa gaacgcccac gaagcgctgg
     1861 agcgcgtcgg gctcggcgac agtgccgagc gcatcgtcgt cgagacctcc tacggcgagc
     1921 ggcgccggct cgaactggcc atggcgctgg cgcagaagcc gaagctgctc ctgctcgatg
     1981 aaccgctcgc cggactctcc cagggcgagc gccagaccgt tcgcaccctg ctcggcacca
     2041 tcccgcgcga cgtgaccatc gtcatgatcg agcacgacat ggacacggcg ctgggctttg
     2101 ccgagcgcat cactgtgctg cattacggcg aggtcatcgt cgaaggcacg cgcgccgagg
     2161 tggtcgcgga tccgcgcacc cgggaggtgt atcttggcga ctgaagcgct cgcgatctcg
     2221 gacatcgata cttattacgg cgacagccac gtgctgcacg gcgtgtcgtt ctcgctgcag
     2281 ccgggccggc tgcttggcct gctcggacgc aacggcgccg gcaagagcac ctgcatgtcg
     2341 acgatcatgg gctttctcaa gccgcggcgt ggctcgatca gcctgtacgg cgaacccgtc
     2401 gccggcctcg cgcccgacgt gatcgcgcgc aagggcatct gcctggtccc gcagggacgg
     2461 cgcatgttcc gcacgctcac tgtgcgcgag aacctgatgg tggcggcgca gtcgcagaag
     2521 aaggacaacg gcgcgggctg gtcgatcggc cgcgtgttcc agacgtttcc ccggctcgcc
     2581 gagcgccacg cccaggtcgc cggctcgctg tccggcggcg agcagcagat gctcgcaatc
     2641 gggcgcgcgc tcatgggcaa cccccgcgtg ctgctgatgg acgagccgtc cgaggggctt
     2701 gcaccacagt tggtggccga ggtcggacgc accatcgcgc agctcaaggc cgaaggacag
     2761 tcgatcgtcc tggtcgaaca gaatatcaag ctgacgctcg acctcgccga tgatgtcgtc
     2821 atcatcaata ccggccgggt agtgtttcgc ggacccgcca gcggcatcaa gctcgacgat
     2881 gccattgtct cccagcacct gggtgtgttc tagagcatgg tcccgaaaag ttgcagactt
     2941 ttcggataag atcatgcgac caaagaaacg caggcagcag acgcgacacc cggaccattc
     3001 atcaggcgta gaaggaacga agcggaaatg gcgacgcaat ggaacaagta cgaagcgacc
     3061 gcggcccgca agcacggcaa accggggcgc gaggtcaggc cgaagagcac caccatcgac
     3121 attcattccc acgtcgccat tccgcaggcc gccgcctacg tcaaaccgca cctcgacatg
     3181 tcgacgatcg cgcttgcgca cttcgcctcg cccgaaacca aggcgctgag ccagaagcag
     3241 gaagaggata tccgcgcgcg cattaccggc tacgacgagc gcctcgccga tctcgacacc
     3301 atgggcatcg accttcagct cgtcatgccg ccgccgaacc agtgcttcta caccgtgccg
     3361 ctcgacatct cggtccaggc ctcgcgcttg gtgaacgatg ggctggcgga atacgtctcc
     3421 cgcaagcccg accgctttgt cgcgctcggc accgtcccgc tcatccatgg cggggaagcg
     3481 gcgaacgagc tcgaacgctg cgtgaagtcg ctcggcatga agggcgtgga aatcctgacc
     3541 aatgtcgcgg gccgagagct ttcggagcca gatttcgcgc cgttctggca gaaggccgaa
     3601 gagctcgacg cgctcgtcgt catccatccc aacggtttca ccgacgccaa acggttcacg
     3661 cggttctatt tcaacaacgt gatcggcaat ccgctcgaaa ccaccatcgc cctgcactac
     3721 ctgatcttcg acggcgtact ggagcggcac cccaggctga agatactcgc ggtgcacggc
     3781 ggcggctatc ttccggccta ttccggccgc atcgatcatg cctggggcgc gcgctcggat
     3841 tcacacggcg acctgccgca gccgccgacc agctatctca agaagattta tttcgacacc
     3901 gtggtgttca cgccttacca gctcgcggag ctggtgcgcc agttcgggac cgaccgcatc
     3961 atcatgggaa cggactatcc gttcgacatg gccgacttcg acccgatcgg ccacgtcgcg
     4021 tcggtcgaac acttcgaccc caccaccatc gccgccatcg ccgggggcaa cgcacgcaag
     4081 ctgctgggca tctagcgtcg attcatctct gtctcacgct gaggagcacg cgtgcgtctc
     4141 g
//
LOCUS       DATDEU010000033         4018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_442725, whole genome shotgun sequence.
ACCESSION   DATDEU010000033 DATDEU010000000
VERSION     DATDEU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442725"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VIV63_00715"
     CDS             complement(<1..350)
                     /locus_tag="VIV63_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123143.1"
                     /translation="MIAFALAMRTLMREWRSGELGVLVLAITVAVAALTGVGFLVNRI
                     GIAVDNQAGEVLAADLRLESGQLMDDKAIDEARRRGLDVARSTGLFSGVFNGDNTPLT
                     SLRAVSDKYPLRGRV"
     gene            complement(361..1023)
                     /locus_tag="VIV63_00720"
     CDS             complement(361..1023)
                     /locus_tag="VIV63_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6123144.1"
                     /translation="MSVLKAESLTKKVTSPEGMLTIVDVVSLDIAAGESVAIVGASGA
                     GKSTLLALLAGLDSPTSGKITLAGADLTSLGEDGRARLRAERVGFVFQSFHLVPSLTA
                     LENVMLPLELAGRRDARAAAAETLGRVGLEHRTGHYPRQLSGGEQQRVAIARAFVTRP
                     AVLFADEPTGNLDTHTGEKIVSLLFDLNAQSNTTLVLVTHDRTVAARCGRVLQMDSGL
                     LK"
     gene            1103..1657
                     /locus_tag="VIV63_00725"
     CDS             1103..1657
                     /locus_tag="VIV63_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY6123145.1"
                     /translation="MLGDSLSAGYGLKIQEGWVNLLSQRLTSEGYGYKVVNASVSGET
                     TQGGLARLPRVLELHKPDIVIIELGGNDGLRGLPLATSRENLRRSIELARGARARVLL
                     VGMLIPPNYGQRYSQEFRDMFTTLASTYSLALVPFLLDRVALEPGFMQDDGIHANAKG
                     QPRMLENVWPKLKPLLVAPRKPAS"
     gene            1686..3356
                     /locus_tag="VIV63_00730"
     CDS             1686..3356
                     /locus_tag="VIV63_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6123146.1"
                     /translation="MEKHWLKSYPPGVRAEIDINEYASLKELIERGLAIHSVRDAYVQ
                     MGKAMTFGELDILSMQFGAFLQHSCGLKKGDRVAIMMPNTLQYPIAIHGALRAGFTVV
                     NTNPLYTTRELEHQLSDSGATVIVILENFAHVLQEVIAHTPIKHVIVTSVGEMLGFPK
                     GNIVDFVVRHKRKQVKPFNLPGSKSFKQALKEGSKVRLQPVKLTHSDIAFLQYTGGTT
                     GVAKGAMLTHGNMVANVLQALEWIGPSFPRDSATLVTALPLYHIFALTANCLLFVILG
                     WRNVLITNPRDFPAFVAELRKHKFAYLSGVNTLFNALLNTPGIETVDFSNLRLTLGGG
                     MAVQEAVAKRWKEVTGKILTQAWGLTETSPAACINIPGEDFNGSIGFPIPSTEISIRD
                     DDGNELGINQVGEICVRGPQVMAGYWNRPDETAKVMIGSDLLRTGDIGRIDERGLVFI
                     EDRKKDMILVSGFNVYPNEVESVAVTHSGVLEAAAIAQADEKSGEVVALFIVKKDQSL
                     TEQEIIEHCRKALTGYKVPKHVYFRTDLPKTNVGKILRRALREELPKS"
     gene            complement(3360..>4018)
                     /locus_tag="VIV63_00735"
     CDS             complement(3360..>4018)
                     /locus_tag="VIV63_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123147.1"
                     /translation="CTYGISRETTQERFEKSLVKAYAASRVRSTRDAGPLSSMADLCR
                     RWYSETGYVDKLGNPKALSWDGRKGTLLKLAERVNGKRNAKEVISNLISHKHIRRIRH
                     GGWVPKAQVIAPSGLDAAQVLRAATMFERLLRTVAYNSERNYRGDVLLEVMAQVPRLP
                     ARDVRSFKKFAKAQGLIFAMSVDDWLESRNIPRNKQRRVPTRQAGVIAFAFEQSALRS
                     "
BASE COUNT          813 a         1225 c         1193 g          787 t
ORIGIN      
        1 acgcggccac gcagtggata tttgtccgag actgcgcgca gtgatgtgag cggggtgttg
       61 tcgccgttga acaccccgct gaaaaggccc gtgcttcgtg cgacatccag gccgcggcgc
      121 cgcgcctcat cgatggcctt gtcgtccatc agttgaccgg actcgagacg aaggtcggcg
      181 gcgagcactt cgcccgcctg attgtcgacg gcaatgccga tgcgattgac caggaatccg
      241 acgccggtca gcgctgcgac cgctaccgtg atcgcgagca ccaggacacc gagttcgccc
      301 gatcgccatt cgcgcatcag cgtgcgcatc gccagtgcga acgcgatcat cgagcggtcc
      361 tcacttgagc agtcccgagt ccatctgcag gacgcgtccg cagcgcgccg ccaccgtgcg
      421 atcgtgggtc accagcacca gcgtcgtatt cgattgcgcg ttgagatcga acaacagcga
      481 cacgattttt tcgcccgtgt gcgtatccag gttgccggtg ggttcgtcgg caaacagaac
      541 cgcggggcgc gtcacgaagg cccgcgcgat ggccacgcgt tgctgctcgc caccggagag
      601 ctggcgcggg tagtgccccg tgcgatgttc gagccccacc cggccgagag tttccgcggc
      661 cgcggcgcgc gcatcgcggc gtcccgcgag ctcgaggggc aacatgacgt tttcgagcgc
      721 ggtaagcgac ggcaccaggt gaaaggactg gaagacgaat ccgacacgct cggcgcgcag
      781 ccgggcccgg ccgtcttcgc caagagacgt caagtccgcg cccgccaggg tgatttttcc
      841 ggaagtgggc gaatccagac cggccaggag tgccagcaga gtcgatttgc ccgcgccgga
      901 tgcaccgaca atggcaactg attcacctgc cgcaatgtcc aatgaaacaa cgtcaacgat
      961 ggtcagcatc ccttccgggc tggtaacctt ttttgtgagg ctttcggctt tgagtacgct
     1021 catcagattc attcagttcg tgttggtctt tgcgtgggtt atgcctgccg gagccgaaac
     1081 cgccgcgcct cgcaccatag tcgtgcttgg cgacagtctc agcgccggat atggcctcaa
     1141 gattcaggaa ggctgggtaa acctgctttc acagcggctc acctccgaag ggtacggata
     1201 caaggtggtc aacgccagcg tcagcggcga aaccacgcaa ggtggactcg cccggctgcc
     1261 gcgcgtgctc gaattgcaca agcccgacat cgtgatcatc gaactcggcg gcaacgacgg
     1321 actgcgcggc ctgccgctcg ccaccagccg tgagaacctg cggcgctcga tcgagctcgc
     1381 acgcggcgcg cgtgcgcgcg tattgctggt aggcatgttg attccaccta actacggcca
     1441 gcgttattcg caggagttcc gcgatatgtt cacgacgctc gcctccacgt attcccttgc
     1501 ccttgtgccg ttcctgctcg accgggtggc gctcgagccc ggattcatgc aggacgacgg
     1561 catccatgcg aacgccaaag ggcagccgcg gatgctcgaa aacgtctggc cgaagctcaa
     1621 gccgctgcta gttgccccgc gcaaacccgc gagctagcct tcgacaccaa gttcgagggg
     1681 tggtcgtgga aaaacattgg ctcaagtcgt atcccccggg cgtgcgcgcc gagatcgaca
     1741 taaacgagta cgcctcgctc aaggagctca tagaacgagg gctcgccatc cactccgtgc
     1801 gcgatgcgta cgtgcagatg ggcaaggcca tgaccttcgg cgaactcgac atcctgtcga
     1861 tgcagttcgg ggccttcctg cagcatagct gcgggctcaa gaagggcgat cgcgtcgcca
     1921 tcatgatgcc taacaccctg cagtacccca tcgccatcca tggcgcactc cgggccgggt
     1981 tcacggtcgt caacaccaac ccgctgtaca ccacccgcga actcgagcat cagctttcgg
     2041 actccggcgc caccgtcatc gtgatcctcg agaacttcgc acacgtgctg caggaggtca
     2101 tcgcgcacac gcctatcaaa catgtgatcg tgaccagcgt cggcgagatg ctcggtttcc
     2161 ccaagggaaa catcgtcgac ttcgtcgtgc gccacaaacg aaagcaggtg aagccgttca
     2221 acctgcccgg atccaagagc ttcaagcagg cgctgaagga aggcagcaag gtgcgcctgc
     2281 agcccgtcaa gctcacccat tcggacattg cgttcctgca atacaccggc ggcaccaccg
     2341 gcgttgccaa gggcgccatg ctcacgcacg gcaacatggt ggcgaacgtc ctgcaggcgc
     2401 tcgagtggat aggcccgtcg tttccgcgcg attccgccac gctcgtcact gcgttgccgc
     2461 tgtatcacat cttcgcgctc acggcgaact gcctcctgtt cgtcattctt ggatggcgca
     2521 acgttctgat caccaatccg cgcgacttcc ctgcgttcgt cgcggaactg cgcaagcaca
     2581 agttcgcgta tctgagcggc gtgaacacgc tgttcaatgc gttgctgaac acgcccggaa
     2641 tcgagactgt cgacttcagc aacctgcgcc tcacgctcgg cggcggcatg gccgtgcaag
     2701 aggccgtcgc gaagcgttgg aaagaagtca ccggcaagat cctgacgcag gcgtgggggc
     2761 tcaccgagac ctcgcctgcc gcatgcatca acattcctgg cgaagatttc aacgggtcca
     2821 ttggtttccc catcccttca accgagatca gcatccgcga tgacgatggc aacgagctag
     2881 gtatcaacca ggtgggcgag atctgcgtgc gcggtccgca ggtcatggct ggctattgga
     2941 atcgcccgga tgaaacggcg aaggtgatga tcggcagcga tctgctgcgt accggcgata
     3001 ttggccgcat tgacgagcgt ggcctggtgt tcatcgagga tcggaagaag gacatgattc
     3061 ttgtgtccgg ctttaacgtt tatccgaatg aggttgagag tgttgccgtc acgcactctg
     3121 gggtgctcga agccgctgcg atcgctcagg cagatgagaa gtcaggtgag gtcgttgcgc
     3181 tgttcatcgt gaagaaggat cagagtctca ctgaacagga aattatcgag cactgccgca
     3241 aggcgcttac ggggtacaaa gtgccgaagc atgtgtactt ccgaacggat ctgccgaaga
     3301 cgaatgtcgg gaagattttg agaagggcgc tgagggagga gttgccgaaa agctgagcac
     3361 tacgagcgca gagcagattg ctcaaatgcg aacgcgatca cgccagcttg ccgcgtcgga
     3421 acacgccttt gtttgttgcg tgggatattt ctagattcga gccaatcgtc aactgacata
     3481 gcaaaaatca gcccttgtgc tttcgcgaat ttcttgaatg atcgaacgtc gcgagctggt
     3541 aatcgtggca cttgagccat gacttcgagc aagacgtcgc cgcggtagtt tctttcgctg
     3601 ttgtaagcga ctgttcgaag cagacgctca aacattgttg cggcgcgaag cacttgtgca
     3661 gcgtcaagac cagagggcgc gataacttgc gcttttggca cccacccgcc atgccgaatt
     3721 cgacgaatgt gcttgtgcga aatcaagttt gaaatgacct cctttgcgtt tcgctttcca
     3781 ttcactctct cggccaactt taaaagcgtt ccctttcgac catcccaaga aagggccttc
     3841 ggatttccca gcttgtccac atagccggtt tcgctgtacc agcgccgaca aaggtccgcc
     3901 atgcttgaaa gagggccggc gtcgcgcgtc gagcgaactc gcgacgccgc gtacgctttc
     3961 accaaggatt tctcgaaacg ctcttgagtg gtttctcgag aaattccgta tgtgcaca
//
LOCUS       DATDEU010000034         3689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1172166, whole genome shotgun sequence.
ACCESSION   DATDEU010000034 DATDEU010000000
VERSION     DATDEU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172166"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VIV63_00740"
     CDS             <1..234
                     /locus_tag="VIV63_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123148.1"
                     /translation="LTPDTPIEGRIVFTKRAKFPKGLPRFTVMLDSIAAEFPRLGAAE
                     LEIAVAKYRPGWAKLKDAAKPSPHFRSNPIAVA"
     gene            complement(231..1304)
                     /locus_tag="VIV63_00745"
     CDS             complement(231..1304)
                     /locus_tag="VIV63_00745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6123149.1"
                     /translation="MQKQAPAVINEETLNGLLGRAITDFGATSLAALVVIGDRLGLYR
                     VLATHGAMTAAELATKSGTHERYVREWLNAHAASGYVNYLADSGRYQFSPEQAMLFAQ
                     EESPAFIVGGFQTALAAGRIVDRLTDAFRTGDGIGWHEHHPDMFPGCARFYKVGYLNH
                     LVQQWIPSLDGVESKLKAGIRVADVGCGVGYSTLIMAKAYPLSHFTGFDYHDESIAAA
                     RQHALEAGLSNVSFEIGKADSYRGHSFEFVTTFDALHDMGDPIAASRHVLKTLAPNGT
                     WLIVEPYAGDRIEENLNPVGRAYYAASTLLCTPCSLSQEGRMGLGAQAGEARLRSVVS
                     GAGFKRFRRASHSPVNLILEARP"
     gene            1448..2449
                     /locus_tag="VIV63_00750"
     CDS             1448..2449
                     /locus_tag="VIV63_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6123150.1"
                     /translation="MDALSALLRAVKLSGAMFYKSRCTAPWCLHAPPSSAFLPYVSPG
                     ATHIIEFHHISEGTAFVRVGAETTPLTAGDIVMIPHGDPHVMGSGIGGAVIDGDKAMP
                     ALMAGEMFLEQFGGGGENTGLVCGYLACDGELIKPVLAGLPRVVRANFREDASGEWFE
                     KTLSHAQEQAAARAPGSDVILAHLAEVLFAEVLRRYLLSLPEGRTGWLAGAGDPAVGR
                     ALAELHQRPGHSWTLDELAQAVGVSRSVLSERFTRYLGQAPMSYLAEWRLEIGAESLR
                     TTSRSVQRVANDVGYESEAAFNLAFKRRFDLPPARYRRDWRAQAKPLVARPAARVAR"
     gene            complement(2452..3243)
                     /locus_tag="VIV63_00755"
     CDS             complement(2452..3243)
                     /locus_tag="VIV63_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY6123151.1"
                     /translation="MTHPALIDIGVNLAHDSFDADRDAVMRRAAEAGVAQMIVTGSSE
                     ASTRSAITLSATHRGVLFATAGVHPHHAADVTLDTLPAIQELARDPHVVAVGECGLDY
                     FRDFSPRDLQRRAFGWQLEIAAATGKPVFLHQRDAHDDFIAILREHRVTRGVAHCFTA
                     GDQERDAYLELGLHIGITGWINDERRGLHLRTVVKGIPADRLLIETDAPYLLPRDIKP
                     APKTRRNEPAWLPYVVRAIAAARGEPAESIAAVTTANARRLFGLA"
     gene            complement(3240..>3689)
                     /locus_tag="VIV63_00760"
     CDS             complement(3240..>3689)
                     /locus_tag="VIV63_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123152.1"
                     /translation="SLPRPALTLAVAGVLMMALAYLLEGVCDIIKNIWTSTFALFTGG
                     FCLTLLGLLMPVTQRPVVRPALQPTRIFGENPLLAYIICFLIAPLIDAHWLGDETAPV
                     SLRGFGQAWFEYFMAPRAASLAFGLCGLAFIFAILLVCHRKRWILKL"
BASE COUNT          621 a         1179 c         1271 g          618 t
ORIGIN      
        1 ctgacgccgg atacgccgat cgaaggtcgc atcgtgttca cgaagcgggc caagtttccg
       61 aagggcctgc cgcgctttac cgtgatgctc gattcgatcg cggcggaatt ccccaggctc
      121 ggcgccgccg agctcgagat cgccgtggcg aaatatcgtc cgggctgggc gaagctcaaa
      181 gacgcagcca agcccagccc gcatttccgc tccaatccaa tcgccgtcgc ttaaggcctc
      241 gcctccagga tcagattcac cggcgagtga ctcgcgcggc ggaatcgctt gaatcccgct
      301 cccgacacca ccgaccgcag gcgcgcttcg cctgcctgtg cgcccaggcc catgcgccct
      361 tcctgtgaca gcgagcaggg cgtgcacaac agcgtggacg cggcgtaata cgctcgtccg
      421 accggattga ggttctcctc gatacgatcg ccggcgtagg gttcgacgat cagccaggtg
      481 ccgttcggcg ccagcgtctt caacacgtgg cgcgacgcgg cgatggggtc gcccatgtcg
      541 tgcaacgcat cgaacgtcgt gacgaattcg aacgaatgcc cacgatacga atccgctttg
      601 ccgatctcga agctcacatt gctcaggccc gcctcgaggg cgtgttggcg cgcggccgcg
      661 atcgactcgt cgtggtagtc gaagccggtg aaatgcgaca gcgggtaggc cttggccatg
      721 atcagcgtcg agtaacccac accgcagccc acgtcggcga cgcggatgcc ggccttgagt
      781 ttgctctcga cgccatcgag cgacgggatc cactgctgca ccaggtgatt gagatatccc
      841 accttgtaga accgcgcgca gcccggaaac atgtcggggt gatgttcgtg ccagccgatg
      901 ccgtctccgg tgcggaaggc gtccgtgagg cgatccacga tgcggccagc ggccagcgcg
      961 gtctgaaagc cgccgacgat gaacgccgga ctctcctcct gggcgaacaa catcgcctgt
     1021 tcgggtgaga actggtagcg gccactgtcg gccaggtagt tcacgtagcc cgaggccgca
     1081 tgggcattga gccactcgcg cacgtagcgc tcatgagtgc cgctcttcgt ggccagctcg
     1141 gcggccgtca tggcgccgtg ggtcgcgagc acgcggtaga ggccgagtcg gtcgccgatg
     1201 accaccagcg cggcgagcga ggttgcgccg aaatcggtga tggcgcggcc gaggaggccg
     1261 ttcagcgtct cttcgttgat caccgcgggg gcttgtttct gcatgttgtc tctccaggtc
     1321 gtttgtgagg cctgaattct gggcagacgc ggagccggcg aatttgccgg gccgccgccg
     1381 ttattgaccg atcgcccgga acggctcgcg cacttgaccg atctgccgca gaatttgccc
     1441 cgcacccatg gacgctctat ccgccttgct ccgcgccgtg aaactctcgg gcgcgatgtt
     1501 ctacaaatcg cgctgcaccg cgccgtggtg cctgcacgcg cctccctcca gcgcgttcct
     1561 gccgtacgta tctcccggag ccacgcacat catcgagttc catcacatca gtgaaggcac
     1621 ggcttttgtg cgcgtggggg cggagacgac accgctcacg gccggggaca tcgtcatgat
     1681 tccgcatggc gatccacacg tcatgggcag cggcatcggc ggcgcggtca tcgacggtga
     1741 caaggcgatg ccggccctga tggcgggtga aatgttcctg gagcagttcg gtggcggtgg
     1801 cgagaacacc ggactcgtgt gcggctatct cgcctgcgac ggcgagctca tcaagcccgt
     1861 gctggcgggt ctgccgcgcg tggtgcgcgc caattttcgt gaagatgctt cgggcgagtg
     1921 gttcgagaag acgctgagcc acgcgcagga gcaggccgcc gcacgcgcgc cgggcagcga
     1981 cgtgatcctg gcgcacctgg ccgaagtgtt gttcgccgag gtgctgcgtc gctatctact
     2041 gagcctgccc gagggccgca cgggctggct cgccggcgcg ggcgatccgg ctgtagggcg
     2101 cgcgcttgcc gagctgcatc agcggccggg gcactcgtgg acgctcgacg aactggcgca
     2161 ggcggtgggc gtgtcgcgct cggtgttgtc ggaacgattc acgcggtatc tcgggcaggc
     2221 gcccatgagt tatctcgccg agtggcgtct ggaaatcggc gccgaatcgc tgcgcaccac
     2281 cagccgaagc gtgcagcgcg tggccaacga cgtgggttac gagtccgaag cggcgttcaa
     2341 tctcgcgttc aagcggcgat tcgatcttcc gccggcgcga taccggcgcg actggcgcgc
     2401 gcaggcgaag cctttggttg cgcgcccggc cgcgcgcgtc gcgcgttaag gttaggccag
     2461 cccgaaaagc cgccgcgcgt tcgcggtggt gacggcggca atggattcgg cgggttcgcc
     2521 acgcgccgcg gcgatggcgc ggaccacgta gggcagccag gcgggctcgt tgcgccgcgt
     2581 ctttggggcg ggcttgatgt cgcgtggcag tagatagggc gcgtcggtct cgatcagcag
     2641 ccggtcggcc gggattccct tgacgacggt gcgcaggtgc aggccgcggc gctcatcgtt
     2701 gatccagccg gtgatgccga tatgcagccc gagttcgaga tacgcgtcgc gctcctggtc
     2761 accggcggtg aagcagtgcg cgacgccgcg cgtcacccga tgctcgcgca ggatcgcgat
     2821 gaaatcgtca tgcgcatcac gctggtgcag gaacaccggc ttgccggtgg cggcggcgat
     2881 ctcgagctgc catccgaacg cgcggcgctg taaatcgcgc ggcgagaagt cccggaagta
     2941 gtcgagccca cattcaccga cggccaccac gtgggggtcg cgcgccagct cctggatcgc
     3001 gggcagcgta tccagggtca catccgccgc gtgatgcgga tgcacgccgg cggtggcgaa
     3061 caacacgccg cggtgcgtgg cagaaagggt gatggcactc cgggtgctcg cttcgctgga
     3121 gccggtcacg atcatctgcg cgacgccggc ctcggccgcg cgccgcatga cggcgtcgcg
     3181 atccgcatcg aagctgtcat gcgcgagatt gacgccgatg tcgatcaggg cagggtgggt
     3241 cacagcttca ggatccagcg tttgcgatgg cacaccagca gtatggcgaa gatgaacgcg
     3301 agcccgcaca gcccaaatgc cagcgaagcc gcgcggggcg ccatgaaata ctcgaaccac
     3361 gcctggccga aaccccgcag cgatacgggt gccgtctcat cacccaacca gtgggcgtcg
     3421 atcagcggcg cgatgaggaa gcagatgatg tacgcgagca gcgggttctc gccaaagatg
     3481 cgtgtgggtt gaagcgcggg ccgcacgacc ggcctttgcg tcaccggcat caggagtccg
     3541 agcagcgtga gacagaatcc gccggtgaac agcgcaaacg tgctggtcca gatgttcttg
     3601 atgatgtcgc agacgccttc cagcagatag gcgagcgcca tcatgagcac gccggcgacg
     3661 gcgagcgtga gagccgggcg cggaaggct
//
LOCUS       DATDEU010000035         2981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1901625, whole genome shotgun sequence.
ACCESSION   DATDEU010000035 DATDEU010000000
VERSION     DATDEU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901625"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VIV63_00765"
     CDS             complement(<1..241)
                     /locus_tag="VIV63_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123153.1"
                     /translation="MTYSGILARSRGLALLAALSAAATLAAVRSADAMPGYDGVWSVV
                     IITKEGICDPSYRYPIRITNGTLGNAGTATVSITGK"
     gene            464..1414
                     /locus_tag="VIV63_00770"
     CDS             464..1414
                     /locus_tag="VIV63_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6123154.1"
                     /translation="MITRRHLLGASAAIAGAVAVGGLRPATAQQRNLGRNRLVLLGTR
                     GGPRITEYKQTPAANLIVWNNVPYVIDTGYGATFKLVETKFPLPALRYTFITHHHSDH
                     NAEFGLLVYNAWATGLQTPVDAYGPAGMKQLAQGFWDAYKFDIETRIADEGRPDLRKL
                     VNIHEYTEGPVMAAGDAKVTALRNLHPPITESFALKFEFPGKTVVFSGDTAYFPPLAA
                     FAKGADILVHEVMYGPALDELVRRNPNAATLMAHLKASHTLAEDVGRIATDAGVKKLV
                     LSHLVPADATGLTAEKWADAVRPTYKGDLVVAHDLMEIPL"
     gene            1457..2692
                     /locus_tag="VIV63_00775"
     CDS             1457..2692
                     /locus_tag="VIV63_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HEY6123155.1"
                     /translation="MPAERFDFPNAAGQKLAALLDRPPGEPRAYALFAHCFTCGKDVH
                     AAKRIAEGLTALDIAVLRFDFTGLGSSDGEFANTTFSSNVADLVAAANELRRTRRAPA
                     ILIGHSLGGAAVLAAASDVPEARAVVTIAAPSDPTHVKGLFADHLEEIGTKGEVEVVL
                     AGRPFRISRAFVDDLAEHRLLDRLPDLHKALLIFHSPTDATVGIENASRIFTAAKHPK
                     SFVSLAGADHLLSRRSDATYVANVIHAWAERYLDMAEVAPDLPDESGAVVVRETRRGR
                     FQQQVTVGRHRFLADEPTDFGGLDSGPGPYDLVLAGLGACTSMTLRLYAERKALPLER
                     VTVELSHSRIHAADCETCETKEGMVDRIERTIALRGNLDAEQRERLLEIADKCPVHRT
                     LTSEVDVRTIERRDADYEP"
BASE COUNT          548 a          969 c          959 g          505 t
ORIGIN      
        1 ccttgcccgt gatgctgacg gtcgcggtgc cggcattgcc taaggtgccg ttggtgatgc
       61 ggatcgggta gcggtaggac ggatcgcaga tgccttcctt cgtgatgatc acgacgctcc
      121 agaccccatc gtagcctggc atggcatcgg ccgaacgaac cgcggcgagc gtggccgccg
      181 ccgagagcgc ggccaacagc gccaggcccc gtgaccgggc cagaattccg gaataagtca
      241 tttgatgccc cttcctactt tcctgccggg cgccgccccc tgggcataac ggcagtgaac
      301 agtgatcttc gtgcgccact tcgttagtcg caaagggacc gcagttggtt caacagcgtg
      361 aacaaattcg aaagagccgt cggataccgg cggtgcgttg ccccggcgcc ttgagctgtc
      421 aagctgtagg ttaaggggca ccatgacccc aggagagcga ttgatgatca cgcgcagaca
      481 tctgttggga gcgagcgcgg cgatagcggg agcggtcgcc gtaggcggcc ttcgccctgc
      541 cacggcgcag cagcgaaatc tgggacgcaa ccgcctggtg ctgttgggga cgcggggcgg
      601 gccgcgcatt accgagtaca agcagacccc ggcggcaaac ctcatcgtct ggaacaacgt
      661 cccttacgtg atcgataccg gctacggcgc gaccttcaag ctggtggaga cgaagtttcc
      721 gctgccggcg ctgcgctaca ccttcatcac gcaccatcac tccgatcaca acgccgagtt
      781 cgggctcctg gtctacaatg catgggcgac cggcttgcag acgccggtcg atgcctacgg
      841 cccggccggc atgaagcaac tcgcgcaggg cttttgggac gcctataagt tcgacatcga
      901 gacgcgcatc gcggacgagg gcaggccgga cctgcgcaag ctcgtcaaca ttcatgaata
      961 taccgaaggc ccggtaatgg ccgcgggcga tgccaaggtg acggcgttac gcaatctgca
     1021 tccgccgatc accgagagct tcgcgctcaa gttcgagttc ccaggaaaga ccgtcgtctt
     1081 ttcgggtgac accgcctatt tcccgccgct tgcagcgttc gcgaaggggg ccgacatcct
     1141 ggtgcacgag gtcatgtacg gaccggcgct cgacgagctg gtgaggcgca accccaatgc
     1201 ggcgaccctg atggctcacc tcaaggcgag tcacacgctc gcggaagacg tcggacgcat
     1261 cgccaccgac gcgggggtga aaaaactggt gctcagtcac ttggtgccgg cggacgccac
     1321 cgggttgacg gcggagaagt gggcggatgc ggtgcgcccc acctacaagg gcgatctggt
     1381 ggtggcgcac gatctgatgg aaattccgct gtgagcgagg cgcgggaccg acgcccgcaa
     1441 tccgcaagag gtaagcatgc cggctgaacg cttcgatttt cccaacgccg cgggccagaa
     1501 gctcgccgcg ctgctcgacc gtccgcctgg cgagccgcgg gcctacgcat tgttcgccca
     1561 ctgcttcact tgcggcaagg acgtgcatgc ggccaagcgc atcgcggaag ggctgacggc
     1621 gctcgatatc gccgtcctgc gcttcgactt caccgggctg ggctcgagcg acggtgaatt
     1681 cgccaacacc acgttttcgt ccaatgtcgc ggatcttgtc gccgcggcaa acgagctgcg
     1741 gcgaacgcga cgagcgcctg cgatcctgat cggccacagc ctcggcggcg cggccgtgct
     1801 cgcggcggcc tcggatgtgc ccgaggcgcg cgcggtggtg acgatcgctg cgcccagcga
     1861 cccgacccac gtgaaggggc tgttcgcgga ccatctcgag gagatcggca cgaagggcga
     1921 ggtcgaggtc gtactggccg gccgtccgtt tcggatcagc cgcgccttcg tcgacgacct
     1981 cgcggagcac aggctgctgg atcgccttcc cgacctgcac aaggcgttgc tgatctttca
     2041 ttcgccgacc gacgccaccg tcggtatcga gaatgcgagc cgcatcttca ccgccgccaa
     2101 gcatccgaaa agcttcgtct cgctggccgg cgccgaccac ctgttgagcc gtcgcagcga
     2161 cgccacctac gtcgccaacg tgatccacgc ctgggccgag cgctatctcg acatggcgga
     2221 agtcgcgccc gacctgcccg acgaatccgg cgccgtcgtt gttcgcgaaa cgcggcgcgg
     2281 tcgcttccag cagcaggtca cggtcgggcg ccatcgcttc ctggcggacg agccgaccga
     2341 cttcggcggc ctcgacagcg gtccaggtcc ttacgatctc gtgctggccg ggctgggcgc
     2401 ctgcacgtcg atgacacttc gactctacgc cgagcgcaag gcgctgccgc tcgagcgcgt
     2461 caccgtcgaa ctcagccaca gcagaatcca cgccgccgat tgcgagacct gcgagaccaa
     2521 ggaagggatg gtggaccgca tcgaacgcac catcgcattg cggggcaacc tcgacgccga
     2581 gcaacgcgag cggctactgg aaatcgcgga taaatgtccg gtgcatcgca cgctgacctc
     2641 ggaggtggat gtccgcacca tcgagcggcg cgacgccgat tatgagccgt agttcggatc
     2701 agcgcgcggt ttcatgtcat cacccgccgt ggcggatatt gttccgtcga tcgcacgatc
     2761 gcgacgaatt cgcagcgcgc tgacttgggg aaccgcgtgc cacttgcgca gttggctcga
     2821 tgtgaacggg cgacatgcgc gtcagcagcg cttggcgatg gcggcatagt cccgtcccat
     2881 ctcacagggt cccaaccaga aaggcttaag ccatgggctt gttttccaaa gacatcaaaa
     2941 cgatgaacga cctgttcgtg cacgctctgc gcgacattta c
//
LOCUS       DATDEU010000036         4094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1172367, whole genome shotgun sequence.
ACCESSION   DATDEU010000036 DATDEU010000000
VERSION     DATDEU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172367"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..2744
                     /locus_tag="VIV63_00780"
     CDS             162..2744
                     /locus_tag="VIV63_00780"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEY6123156.1"
                     /translation="MAENLVIVESPAKAKTIKKYLGKDFEVLASYGHVRDLVPKEGAV
                     DPDKKFAMTYRSIERNEKHVDAISRALKKSKNLFLATDPDREGEAISWHLRELLEERG
                     DLKGKEVKRVVFYEITKGEVQRAVAEPRDLSLDLVNAQQARRALDYLVGFNLSPLLWK
                     KVRRGLSAGRVQSPALRMICEREDEIGKFIPQEYWTLEAEGEHSAQNFPLKLVEFDGK
                     KVEQFSFTNEDAARGVEKSLNDAAQGFLHVLSIDKKQRKRNPSPPFTTSTLQQEAARK
                     LGYSAQRTMRLAQQLYEGVDFGEGAIGLITYMRTDSLSLAVDAVREMREVIAQEYGAE
                     AVAEEPRIYKTKSRNAQEAHEAIRPTSAAVLPSKLEGKVDPDQYKLYALIWKRAVASQ
                     MAHALFDTVAVDMVPSKQAEAGNGAGGRHVLRANGSTLVKPGYMAVYQEDVDDGKPEN
                     ENDRILPAMKDGDVVTLLNVHGDQHFTEPPPRYSEASLVKALEEHGIGRPSTYATIIS
                     TLKDREYVDMDNRRFIPTDIGKIVGAFLSKHFHKYVEYGFTASLEDELDAVSRGEEDW
                     TLPLEKFWKPFITQVEHIEKNVSREEVAQARELGKHPESGKPITVRMGRYGPFVQIGT
                     KDDEEKPKFAGLRPGQKMDLIKLPEALELFKLPVSLGTTAEGEPVQSNIGRFGPYIKY
                     GAKYVSLKPPDDPYTVTLERALEVIRLKKEADANRIIQDFGVDSIQVLNGRYGPYITD
                     GKRNARIPKDTDPKAITLEVCRELLAVAPPRGSRFGRKKTAKEQAPAKDAAVDAASGS
                     KAKKKSATKAAQKSDVTDVSAAKAERKSTPAKKKSAARTTAKSATTVAKKTSSKAKKK
                     ESKA"
     gene            2970..>4094
                     /locus_tag="VIV63_00785"
     CDS             2970..>4094
                     /locus_tag="VIV63_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY6123157.1"
                     /translation="MKSKRVGHGHVKWAVCSALLSATTLAAAGPRGLTLDGGAHLSSI
                     SLESPIVDASYESFVLHPSLANVSGRQTVLVRLRTPGVAESNGAITRSQVESEQAALI
                     SRLVARAPSANVVASVQLVLNAIVMDVDGGDLAELARDTAITRIVGVSDYAHDLSETV
                     PYIGATTAQSLGATGQGVRVAVIDSGIDYTHANLGGPGTQASYEAAWAPLPAAAPFIP
                     VVPAGTGYLVVDDPGTTADNGLYPSAKVIGGYDFVGESWPNTGLLPDPDPIAAPDATT
                     FGGHGTHVADIIGGVGGVAPGVKFYAIKVCSAPATSCSGIALLQGMDFALDPNGDGSL
                     SDHVDIINMSLGSPYGQPFDDDLSAAVDNASAAGVLTVASA"
BASE COUNT          881 a         1306 c         1242 g          665 t
ORIGIN      
        1 atcgtcatcg accggctcgt cgctctcgat ggcgacgacg tcgacatcga ccggctcaag
       61 tggctggtgc tgatggtgct ctcgagccag ccgggccagg aagcagcctg tgcgcgcatg
      121 gaagacctgc tgttcgacga aacggactcg ttgccgaact gatggctgaa aacctagtca
      181 tcgtcgaatc gccggcgaaa gcaaaaacca tcaagaagta cctcggcaag gatttcgagg
      241 tgctggcctc gtatggccac gtccgcgatc tcgtgccgaa ggaaggcgcg gtcgatccgg
      301 acaaaaagtt cgcgatgacc tatcgctcca tcgagcgaaa cgagaaacac gtcgatgcca
      361 tctcgcgtgc gttgaagaaa tccaagaacc tctttctggc aaccgaccct gaccgggaag
      421 gcgaagccat ttcctggcac ctgcgtgagc tgctcgaaga gcgcggcgat ctcaagggca
      481 aggaagtcaa acgcgtcgtg ttctacgaaa tcaccaaggg cgaggtgcaa cgcgccgtcg
      541 ccgagccgcg cgatctgtcg ctcgacctgg tgaacgccca gcaggcgcgt cgcgcgctcg
      601 actacctggt cggtttcaat ctctcgccgc tgctctggaa gaaagtgcga cgcggactgt
      661 ctgccggtcg cgtgcagagt ccggctttgc gcatgatctg cgagcgcgaa gacgaaatcg
      721 gcaagttcat tccgcaggaa tactggacgc tcgaagccga aggcgaacac agcgcgcaga
      781 atttcccgct caagctcgtc gagttcgacg gcaagaaagt ggagcagttc agcttcacga
      841 acgaagacgc cgcgcgcggc gtcgaaaagt cactcaatga tgccgcgcag ggtttcctgc
      901 acgtcctgtc catcgacaag aagcagcgca agcgcaatcc gtcgccgccg ttcaccacat
      961 ccacgttgca gcaggaagcc gcgcgcaagc tgggctactc cgcccagcgc accatgcgcc
     1021 tggcccagca gctatatgaa ggtgtcgatt tcggcgaagg cgccatcggc ctcatcacct
     1081 acatgcgtac cgattcgctg agcctcgcgg tcgacgcggt acgcgagatg cgcgaagtca
     1141 tcgcgcagga atacggcgcc gaagccgtcg ccgaagagcc gcgcatctac aagacgaagt
     1201 cgcgcaacgc gcaggaagcg cacgaggcca ttcgccccac atccgccgcg gtgcttccat
     1261 cgaagctcga aggcaaggtc gatccggacc agtacaagct ctacgcgctc atctggaaac
     1321 gcgcggtcgc ctcgcaaatg gcacacgcgc tgttcgacac cgttgctgtc gacatggtgc
     1381 cgagcaagca ggccgaagcg ggcaacggtg ccggcggccg ccatgtcctg cgcgcgaacg
     1441 gctccacgct cgtgaagccg ggctacatgg ccgtgtacca ggaagacgtc gacgacggta
     1501 aacctgaaaa cgagaacgac cgcatcctgc ccgccatgaa agacggcgac gtggtcactc
     1561 ttcttaatgt gcacggcgac cagcatttca ccgagccgcc gccgcgctat tccgaagcat
     1621 cactcgtgaa ggcgctcgaa gagcacggca tcggccgtcc ctcgacctac gccaccatca
     1681 tctcgacgct caaggatcgc gagtacgtcg acatggacaa ccggcggttc attccgacgg
     1741 acatcggcaa gatcgtcggc gcttttctct ccaagcactt ccacaaatac gtggaatacg
     1801 gcttcaccgc ctcgctcgaa gacgagctcg acgcggtgtc gcgcggcgaa gaagactgga
     1861 ccctgccgct cgagaaattc tggaagccgt tcatcacgca ggtcgagcac atcgagaaga
     1921 acgtctcgcg cgaagaagtc gcgcaggcgc gtgagctcgg caagcacccg gaaagcggca
     1981 agcccatcac cgtgcgcatg gggcgttacg gtcctttcgt gcagatcggc acgaaggacg
     2041 acgaagagaa gccgaagttc gcgggtctgc gtcccggcca gaagatggat ctcatcaagc
     2101 tgcccgaggc gctcgagctc ttcaaactac cggtgagcct gggcacgacc gccgaaggcg
     2161 agcccgtcca gtcgaacatc ggacgcttcg ggccctacat caagtacggc gccaagtacg
     2221 tttcgctgaa accgccggac gacccgtaca cggtcacgct cgaacgcgcg ctcgaagtca
     2281 tccgcctgaa gaaggaagcg gatgcgaacc gcatcatcca ggacttcggc gtcgacagca
     2341 tccaggtgct gaacgggcgc tacggtccgt acatcaccga cggaaagcgc aatgcgcgaa
     2401 ttccgaagga cacggatccg aaagccatca cgctcgaggt gtgtcgcgaa ctgctggctg
     2461 ttgcaccgcc gcgcggctcg cgcttcggcc gtaagaaaac tgcaaaagaa caggcgccgg
     2521 cgaaagacgc ggccgttgac gcagcgtcag gatcgaaggc aaagaaaaaa tccgcaacga
     2581 aagctgcaca aaagtcggac gtgaccgacg tgagcgcagc gaaagcggaa agaaaatcga
     2641 cacctgcgaa aaagaaaagc gcggcgcgta ccacagcgaa gagcgcaact accgttgcca
     2701 agaaaacttc atcgaaggcc aagaaaaaag agagcaaggc ctgactcgct tatgttcaat
     2761 ttcgtggttc cacgcgattg aattaagtcc tgacgcggag tagcgtcgcc agcgggtcct
     2821 ggatatcacg gtgcgtaacc ttcgatgaat gaaggaacgc actgctcgcg gcatctaaga
     2881 agaaccgtcg cgctccccgt ggctcatgtc tgtttaagac ttgggctcgg cgtatgtccg
     2941 gaataacagg ttctcagggg attattcaaa tgaagagcaa acgtgttggt cacggccacg
     3001 tgaagtgggc cgtgtgcagc gcgttgttgt cggcgacgac gctcgcggcc gctgggccgc
     3061 gtgggttgac gctcgatggc ggcgcgcatc tttcctcgat ttctctcgaa tcaccgatcg
     3121 tcgatgcgag ctacgaaagc ttcgtattac atccgagcct cgcgaacgtt tccggccggc
     3181 agacagtgct ggtccgactg cgtacgccgg gcgtcgccga atcaaacggc gcgatcaccc
     3241 gctcgcaggt cgaatcggaa caggccgcgc tgatcagccg cctggtcgcg cgcgcaccga
     3301 gcgcgaacgt cgtcgcgagc gttcagctcg tcctcaacgc catcgtgatg gacgtcgacg
     3361 gcggcgatct tgccgagctc gcgcgtgaca ccgcgatcac ccgcatcgtc ggagtctccg
     3421 actatgcgca cgacctctcc gaaaccgtgc cgtacatcgg cgcgacgacc gcacaatcgc
     3481 ttggcgccac gggccagggt gttcgcgtcg ccgtgatcga cagcggcatc gactacacgc
     3541 acgccaatct cggcggaccc ggcacgcagg cgtcgtacga agcggcatgg gcgccgctcc
     3601 cggccgcggc tccgttcatc ccggtggtac cggcgggcac cggctatcta gtggtcgacg
     3661 acccgggcac caccgctgat aacggcctct acccgagcgc caaggtcatc ggtggctacg
     3721 acttcgtcgg cgagagctgg ccgaacacgg gcctgctgcc cgatccagac ccgatcgccg
     3781 cccccgatgc gaccaccttc ggcggtcacg gcacgcatgt cgccgacatc atcggcggcg
     3841 tcggcggtgt ggccccgggc gtcaagttct acgccatcaa ggtgtgctct gctccggcca
     3901 cgtcctgcag cggtatcgcg ctgctgcaag gcatggactt cgcgctcgac ccgaatggcg
     3961 acggcagcct cagcgatcac gtcgacatca tcaacatgtc gctcggttcg ccgtacggtc
     4021 agccgttcga tgacgacctc tccgccgcgg tggacaacgc ctcggctgcg ggtgtcctca
     4081 cggtggcgtc cgcc
//
LOCUS       DATDEU010000037         2945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1756875, whole genome shotgun sequence.
ACCESSION   DATDEU010000037 DATDEU010000000
VERSION     DATDEU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756875"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VIV63_00790"
     CDS             complement(<1..714)
                     /locus_tag="VIV63_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6123158.1"
                     /translation="MKFRFLAIALLLTQSAVAQEPVFDVHVHLRDGETSLRAYESDVA
                     AAKVNLAGLGAMWFGGPHQAPQGEPAKVRASNDALIAMAAKHPKVVPIATVHPYDGQA
                     AIDELSRVAARGVKVLKIHPHTQRFDASDPRVLALVKHAGSLGMVVLMDNANILPGDS
                     ERLFNLAVVAPKTKFIFAHMGAANFRFWNILALARTADGFFADNIYFDVSGTALLVAD
                     SPIEQEFVWTLRNVGVDHVL"
     gene            complement(746..1645)
                     /locus_tag="VIV63_00795"
     CDS             complement(746..1645)
                     /locus_tag="VIV63_00795"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6123159.1"
                     /translation="MKATSQSDYTRRIERVIAAVAESLEQERDLPSTATLAGIANFSP
                     FHFMRVYRALAGESLGTTVQRLRLARAAHLLAASAAPISEVSGRVGFETPQAFARAFR
                     QHFGVAPSEARAANLASATAIAAPAPPHSQPAIRVDVVALKPFRVAVKRTEGVYSKLN
                     EAYRGLFEWMARRGALESIDGIWGVPQQDRRDTPEANYLFDCGLATRVALSAGEGIAL
                     ADLGGGNYLRYLHEGSFDRLDESHDAIFRELFTHPEWRLRDAPILHEYLNDPDETPEP
                     DLRTHIYVPISEPGVVARSSTAP"
     gene            1770..1961
                     /locus_tag="VIV63_00800"
     CDS             1770..1961
                     /locus_tag="VIV63_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEY6123160.1"
                     /translation="MFNTGYNGLLGLLILIGDIWAIINIFQSSASNEKKLLWTLVVIL
                     LPLLGLILWFFLGPRGGKT"
     gene            2121..>2945
                     /locus_tag="VIV63_00805"
     CDS             2121..>2945
                     /locus_tag="VIV63_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123161.1"
                     /translation="MKTIFLALAALAFAPVTFAAPPAAPVVTVGAHSIKQLQFDWPSV
                     PGVATYELWFRAAPGAQWVKYTETIAQRPIIRITVSVHLLDWRVARYRVAACNPSGCT
                     NSAEVGVNELALDATGYLKQNSAAEGRWFGQVVAMSADGYTAAVATGQSSGGTADNGI
                     VYLYRKTSTTAPWRLEARLIPSLFRRGSSQPYGTNTSLAVNGDGTLVAYGRFTEGIGG
                     QSANGAVYLFRRNGTTWTQEQRLAGGGANDRFGNAVDLDDSGNTLAVWHRADSANGY"
BASE COUNT          536 a          947 c          936 g          526 t
ORIGIN      
        1 cagcacgtga tcgacgccga cattgcgcaa cgtccagacg aactcctgct cgatcggcga
       61 atccgccacg agcagtgcgg ttcccgacac atcgaagtag atgttgtccg cgaagaatcc
      121 gtcggccgtg cgcgccaacg cgaggatgtt ccagaaccgg aagttcgccg cacccatgtg
      181 cgcaaagatg aattttgtct tcggcgccac cactgcgaga ttaaagagcc gctcgctgtc
      241 gccgggcagg atgttggcgt tgtccatgag caccaccatt ccgagcgagc ccgcatgctt
      301 cacgagtgcc agcacgcgcg ggtcgctggc gtcgaagcgt tgcgtgtgag gatggatctt
      361 cagcactttc acgccgcgtg ccgcgacgcg cgagagctca tcgatcgctg cctgcccgtc
      421 gtacggatga acggtggcaa tcggcaccac tttcggatgc ttcgcggcca ttgcgatgag
      481 cgcatcgttg ctcgccctca ccttcgcggg ctcgccctgc ggagcctggt gcggcccgcc
      541 aaaccacatg gcgccgagtc cggcgaggtt gaccttcgcg gctgcgacgt ctgactcgta
      601 agcgcgcagc gaagtctctc catcgcgcaa atggacatgc acgtcgaaga cgggttcctg
      661 tgcgacggca gactgggtca acagcaaggc gatcgcgagg aatctgaact tcacgcaggt
      721 cactccatga ttgtggcctg gcagtttatg gcgcggtgga tgagcgggca acgacgccgg
      781 gttcactgat cggaacgtag atgtgcgtgc gcaagtcggg ctccggggtc tcgtcgggat
      841 cgttcagata ctcgtgcagg atcggcgcgt cgcgcagccg ccattccgga tgagtgaaga
      901 gctcgcgaaa gatcgcgtcg tgcgattcgt cgaggcgatc gaaggatcct tcgtgcaggt
      961 atcgcaggta gttgccgccg cccaggtcgg caagcgcgat gccctcgccg gcgctcagcg
     1021 ccaccctggt tgccagaccg cagtcgaaca ggtagttcgc ttccggcgtg tcgcgccgat
     1081 cctgttgcgg cacgccccag atgccgtcga tcgattccag cgcgccgcgc ctggccatcc
     1141 actcgaagag accgcgataa gcctcgttca gtttgctgta cacaccttcg gtgcgcttca
     1201 ccgccacgcg gaagggtttg agggcgacga catcgacacg aattgcgggt tgcgaatgcg
     1261 gaggcgccgg cgcagcgatt gccgtcgcgc tcgccaggtt cgccgcccgt gcttcactcg
     1321 gcgccacacc gaagtgctgg cggaacgccc gtgcgaacgc ctgcggcgtt tcgaagccga
     1381 cgcggccgga cacctccgag atgggcgctg cgctggcggc gagcaaatgc gccgcccgag
     1441 cgagacgcaa gcgctgcacc gtcgtaccca gcgactcgcc agccagcgca cggtagacgc
     1501 gcatgaaatg gaacggcgag aagttcgcga tgccggcgag cgtcgccgtg gaaggcagat
     1561 cgcgctcctg ctcgaggctt tccgccaccg cggcgataac acgttcgatg cggcgtgtgt
     1621 aatcagactg gctcgtggct ttcatgcgct tctccgaagt ggtgaagaaa gcatgccaaa
     1681 ggccggcgcg tcacgcggtt cggatcgtgc tcggttgcga ttgcatccct cctcacaaag
     1741 cgcagaatgc agcgaggagg aatcaaacca tgttcaacac cgggtacaac ggccttctgg
     1801 gcctgctgat actgatcggc gacatctggg cgatcatcaa catcttccag agctcggctt
     1861 cgaatgagaa gaagctgctg tggacgctgg tggtgatact gctgccgctg ttggggttga
     1921 ttctgtggtt ctttctgggg ccgcggggtg ggaagacgta gggggcgcag ttccccacgt
     1981 cagacgatag aaccaggcgg cgccgcctgc gcatcgccct gacagtattt gccctgtcgc
     2041 ctccaagccg ccgctgatac gactcgcccg aacacccagc tagtttggtc aaagaccatc
     2101 cagtccggtc tgagggagtc atgaaaacca ttttcctagc attggccgcg ctcgcgtttg
     2161 cacctgtgac attcgccgcg ccaccggccg cgcctgtcgt caccgtgggc gcccacagca
     2221 tcaagcaact gcagttcgac tggccttccg tgccaggggt tgccacttac gagctatggt
     2281 tccgcgccgc gcccggcgcc cagtgggtga aatacaccga aaccattgcg cagcgtccga
     2341 tcatccgcat cacggtgtcc gtccatctgc tcgactggcg cgtcgcgcgc taccgggtgg
     2401 cggcctgcaa tccgtccggc tgcacgaaca gcgccgaggt cggcgtcaac gagctcgcgc
     2461 tcgacgccac gggatatctc aagcagaact ctgctgctga aggccgatgg ttcggccagg
     2521 tcgttgccat gagcgccgac ggctacaccg ccgccgtcgc cacgggccaa tcttccggcg
     2581 gcaccgccga caacggcatc gtttacttgt atcgcaagac cagcacgacg gcgccgtggc
     2641 gcctcgaagc gcgtctcata ccgagcctgt ttcggcgagg cagctcgcaa ccctatggca
     2701 cgaacacctc gctcgcggtg aacggtgatg gaaccctggt cgcatacggc cggttcaccg
     2761 aaggcatcgg cggccagtcg gcgaacgggg ccgtctatct attccgccgc aacggcacca
     2821 cgtggacgca ggaacagcga ctggccggtg gcggcgccaa cgaccggttc ggcaatgccg
     2881 tggatctcga cgattcgggt aatacgctcg cggtctggca tcgcgccgac agcgccaacg
     2941 gatac
//
LOCUS       DATDEU010000038         2968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_881752, whole genome shotgun sequence.
ACCESSION   DATDEU010000038 DATDEU010000000
VERSION     DATDEU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881752"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /gene="flgL"
                     /locus_tag="VIV63_00810"
     CDS             complement(<1..712)
                     /gene="flgL"
                     /locus_tag="VIV63_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEY6123162.1"
                     /translation="MRVSTAGMHRTTIDAILEHQVKLSKTQTQVTTGKKFQTAAEDPI
                     GSARASALDRKLADNAQYGRNSNMLQSRLSYEEQTLADITSVLQSARDSALAGANATL
                     GQSERKMIANQVRQNLAALMDMANRQDGNGEYLFAGTSSATRPFAQGASGVIYQGDQT
                     TRQIRISSTQALTDVHTGVDAFMGITERNGVFRTTVSAANTGNATISVGTVTNPTAWV
                     AGNYTLQFTSATDWQVVDD"
     gene            complement(716..2605)
                     /gene="flgK"
                     /locus_tag="VIV63_00815"
     CDS             complement(716..2605)
                     /gene="flgK"
                     /locus_tag="VIV63_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEY6123163.1"
                     /translation="MADVLSTGLSSLRALQRALDTTSHNIANVSTEGYTRQRVEFSSR
                     RPESSGSGWIGTGVNVSTVRRVYDQFLAQQTRTSGTNLARLDAYASQATRIDNLLGDS
                     ENGLSASIQSFTDAINEVSTTPASIPARQVLIAEGRALAERLKSYDSRLRNMSTEMNG
                     RLGVEAQEITTLAQGVAKLNGEIAVSFQQSGQPPNDLLDQRDALIDELSSKVGVTVVA
                     EGDSTLNVFIGNGQPLVLGNVASQVTTQVDPLDPERTLLALRTPSGTVDLSRVVSGGT
                     LGGLLDWRSQMLDPARNELGRIGVAVAMQVNAQHREGMDLTGAMGGNFFNIGAATATA
                     ATANTGTAAVTVTRTNLGALTTNDYVLARTTTGYSLSRRDTGAAVAFTGTGTVGDPIL
                     ADGLSIVVGAGIATGDQFTIRPTRDALAGMSVAITDPSRVAAAAPIRAGAATTNSGTG
                     TITNGEVLDPTNAALLTTVNIQFTSATTYSVNGAGSFTYTAGSNIDVNGWRVQVNGTP
                     AVGDTFTVRSNAGATGDNRNAFALADALRSNVLDGGTKSAFSAVEQLTADVANSTRTA
                     QVNRDAEAVIHDSDIAERDSVSGVNLDEEAANLLRYQQAYAAAAQIISAAQEMFDTLM
                     NAVRR"
     gene            complement(2598..>2968)
                     /locus_tag="VIV63_00820"
     CDS             complement(2598..>2968)
                     /locus_tag="VIV63_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEY6123164.1"
                     /translation="GIKAGSSWKGASVQANTEEFAGGVASRVDANFRAYGSSRESVED
                     YVRVIRDNPRYASALNTGSDVQAFATALQKGGYATDPEYARKLVSVAAEVGQRVAANP
                     FKSGSAEPIPAQGPLRMSNG"
BASE COUNT          435 a          934 c         1036 g          563 t
ORIGIN      
        1 tgtcgtcgac cacctgccag tcggtggcgc tggtgaactg cagcgtgtag ttgcccgcga
       61 cccaggcggt ggggttcgtc accgtgccga cgctgatggt cgcattgcca gtgttggccg
      121 ccgagaccgt cgtgcggaac acgccgtttc gctcggtaat gcccatgaag gcgtcgacgc
      181 cggtgtgcac atcggtcagc gcttgcgtgc tgctgatgcg gatctgccgc gtggtctgat
      241 caccctggta gatgactccg ctggcgccct gcgcgaacgg ccgggtcgcg ctcgacgtgc
      301 cggcgaacag gtactcgccg ttgccatcct ggcggttggc catgtccatg agggccgcga
      361 gattctgccg cacctggttg gcgatcatct tgcgctctga ctgaccgagc gtggcattcg
      421 cgccggccag ggccgagtcg cgtgcgctct gaagcacgga ggtgatgtcc gccagcgtct
      481 gttcttcgta gctgagacgg ctctgcagca tgttcgagtt gcgcccgtat tgcgcgttgt
      541 ccgcgagttt gcggtccagg gccgacgcgc gggcggagcc gatcgggtct tcggccgcgg
      601 tctggaactt cttgcccgtg gtgacctggg tctgcgtctt cgagagcttg acctggtgct
      661 cgaggatggc gtcgatcgtg gtgcgatgca tgccggcggt agacacacgc atggactatc
      721 tcctcaccgc gttcatgagc gtgtcgaaca tttcctgggc ggccgagatg atctgcgcag
      781 cggctgcata ggcctgctgg tatcgcaaca gattggcggc ctcttcgtcg aggttgactc
      841 ccgacaccga atcgcgctcg gcgatgtcgc tgtcgtgaat cacggcctcg gcgtcgcgat
      901 tgacctgcgc cgtgcgcgtc gaattggcga cgtcggcggt gagttgctcg acggccgaga
      961 acgcggattt ggtgccgcca tcgagcacgt tcgaacgcaa cgcatcggcg agtgcgaacg
     1021 cattgcggtt gtcgcccgtg gcgcccgcgt tgctgcgcac cgtgaacgta tcgcccacgg
     1081 cgggagtgcc gttgacctgc acgcgccagc cgttgacgtc gatgttcgag ccggccgtgt
     1141 acgtgaagct gccggcgccg ttcaccgagt acgtggtcgc cgacgtgaac tggatgttga
     1201 cggtcgtgag cagcgcggcg ttcgtcggat cgagcacttc gccgttggtg atcgtgccgg
     1261 tgcctgaatt cgtcgtggcc gcgcccgcgc gaatcggcgc cgcggcggcg acgcgcgagg
     1321 ggtcggtgat cgccacgctc atgccggcaa gcgcatcgcg cgtgggccga atcgtgaatt
     1381 gatcgccggt cgcgatgcct gcccccacga cgatcgacag accgtcggcc aggatgggat
     1441 cgcccacggt gcccgtgccg gtgaacgcga cggccgcgcc cgtatcgcgg cgactgagcg
     1501 agtagcccgt ggtcgtgcgc gcgagaacgt agtcgttagt cgtgagcgcg ccgagattcg
     1561 tgcgcgtgac ggtgacggcg gcggtgccgg tgttggcggt ggccgccgtg gccgtggccg
     1621 cgccgatgtt gaagaagttg ccgcccatcg cacccgtgag gtccatgccc tcgcggtgct
     1681 gggcattgac ctgcatcgcg acggcgacgc cgatgcgccc gagctcgttg cgcgccggat
     1741 cgagcatctg gctgcgccag tcgagcaatc cgccgagcgt gccgccggac acgacgcgcg
     1801 acagatccac ggtacccgaa ggtgtgcgca gcgcgagcaa ggtgcgttcc ggatcgagcg
     1861 ggtcgacctg cgtggtgacc tgcgaggcca cgttgccgag caccaggggc tggccgttgc
     1921 cgatgaagac attcagagtg gagtcgcctt cggcgacgac ggtgacaccg accttgctgg
     1981 acaactcgtc gatgagcgca tcgcgctggt cgagaaggtc gttaggcggc tggccgctct
     2041 gctggaaaga gacggcgatc tcgccgttga gtttcgcgac gccctgcgcg agcgtggtga
     2101 tttcctgcgc ttcgacgccg agccggccgt tcatctcggt cgacatgttg cgcaggcgcg
     2161 agtcgtaact cttgagccgc tcggcgagcg cgcggccttc ggcgatcagc acctggcgcg
     2221 ccgggatcga cgccggcgtg gtggacactt cgttgatcgc gtcggtgaaa ctctggatcg
     2281 aggccgacaa tccgttttcg gagtcgccta acaggttgtc gatgcgggtg gcctgcgacg
     2341 cgtaggcgtc gagtcgcgcg agattggtgc cgctggtgcg cgtctgctgg gccaggaact
     2401 ggtcgtacac gcggcgcacg gtcgacacgt tgacgccggt gccgatccat cccgagccgg
     2461 acgattccgg ccggcgcgac gagaactcca cgcgctggcg ggtgtaaccc tccgtcgaga
     2521 cgttcgcgat gttgtgcgag gtcgtatcga gtgcccgctg caatgcgcgc aaactcgaca
     2581 gccccgtgct taacacgtca gccattactc atcctcagag ggccctgggc cggtatcggc
     2641 tctgcggagc ctgacttgaa cggattggcg gcaactcttt gccccacttc agcagcgacc
     2701 gagaccagct tccgggcata ttccggatcg gtggcgtaac cgcccttctg gagcgccgtg
     2761 gcgaaagcct gcacgtcgct gcccgtattc agcgcgctgg cgtaacgcgg gttgtcgcga
     2821 atcacgcgga cataatcctc gacgctctcg cgcgaagaac cgtaggcgcg aaagttcgca
     2881 tcgacgcggc tggccacgcc tccggcaaac tcttccgtgt tcgcctgaac gcttgcgccc
     2941 ttccagctcg atccggcctt gatgccga
//
LOCUS       DATDEU010000039         3524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2049284, whole genome shotgun sequence.
ACCESSION   DATDEU010000039 DATDEU010000000
VERSION     DATDEU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049284"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VIV63_00825"
     CDS             <1..1382
                     /locus_tag="VIV63_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6123165.1"
                     /translation="FHDSLTLLANRSLFSDRVHQAVRHIADGMTPAVLFIDLDNFKTV
                     NDSLGHSAGDQLLRGFAHRLVQCTRAGDTVARLGGDEFAVLIDHAPNLEAAMAVARQV
                     LEVCRQPFDIDGRPLRIGCSVGVALADRVSNVERLLRNADAAMYSAKSHGKGHAEVFQ
                     PEMLRAARRRMRIENELATAIEQQQLEVHYQPIVDLNSRHLVGVEALMRWRHPTRGMV
                     MPGDFIPLAEETGQIVAMTQWIIGRACADLARLQKEIAHGAGLRVSVNVSSRYLNHGN
                     VVEDVRNGLAAQRLSPDCLILEVTESLLLENSARIEAIFQELKRIGVRLALDDFGTGY
                     SSLAYLHRFPIDILKIDRSFVERLAGEAEGAGGVDAVALARAILSLADALGLDTVAEG
                     IEHDAQRDTLLELGCKTGQGYSFGKAMPVGEVLDAAVTRRRSLLAGNVPGQIEYSATG
                     RFRGMTESG"
     gene            1573..3372
                     /locus_tag="VIV63_00830"
     CDS             1573..3372
                     /locus_tag="VIV63_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6123166.1"
                     /translation="MKSVETRSLDVGALPERSTESVLRAVCLRLGCTAATAVFPREDS
                     RVVWGRSEEDVAFAREQLSIVGDELARRAIETKKPVATNKLKHVSGGQIACKLVAVPL
                     IARGGPAGALIIFNRAEEPSFDEFTVRRLGRFARLLARNATQDLDSLTGLLSWSGFKS
                     RVDNWQRNAPPDAMVSMLYGDIDRLHLINDLAGFSDGDRVIARCGVAIRRALVGGESF
                     ACRLSGDRFTVFLPHMNLAQARLLADRICKEVSTAAAAKNEPNHPVSMSWGAVSTGVG
                     DLHLDHRIAEAEIACKTAKDRGRGRVEVYQDTDQSMVRRHDEIGLIGAVREALRLERI
                     VVYAQPITPLINHSLPTTYELLVRIVDAKGNIVEAADFMSAATRYQILPEIDRAVCER
                     AFEQLSRNTSSAGPLRVSINLSGPTLSDPDFLEWLLASMAKHGIDGTCLVFEITEAAA
                     AANLSHVQQFIRRMTSHGVRFALDDFGTGVSSLAYLKNLEINTIKLDGSYVVDVMTNP
                     RSQALVRAIVQLAEAMGITTVAEYVESTIVRDRLASLGIHFGQGYALGRPGPLDDVLA
                     AVADRAPEGRDRPFTDNKAAFTPSGIPIMFKHA"
BASE COUNT          622 a         1117 c         1156 g          629 t
ORIGIN      
        1 cgtttcatga ttctctgacg ctgctcgcca atcgttcatt gttctccgat cgcgtgcatc
       61 aggccgtccg gcacattgcc gatggcatga cgccggcggt gctgttcatc gacctcgaca
      121 acttcaagac ggtcaacgac agcctggggc acagcgccgg cgaccagctc ctgcgcggct
      181 tcgcgcatcg actggtccag tgcactcgtg ccggcgacac ggtggcgcgt ctcggcggcg
      241 acgaattcgc cgtactcatc gatcacgcgc ctaacctcga ggccgcgatg gccgtggcgc
      301 gccaggtgct cgaagtgtgc cgccagccgt tcgacatcga cggccggccg ctgcgcatcg
      361 gctgcagcgt cggggttgca ctggccgacc gtgtcagcaa cgtcgagagg ctgctgcgga
      421 atgcggatgc cgcgatgtat tcggccaaat cgcacggcaa gggccacgcc gaagtattcc
      481 agccggagat gctgcgtgcg gcgcggcggc gcatgcggat cgagaacgaa ctcgcgacgg
      541 ccatcgagca gcaacagctc gaggtgcatt accagcccat cgtcgacctg aattcacggc
      601 acctggtcgg tgtcgaggcg ctgatgcgct ggcgccatcc gacgcgcggc atggtcatgc
      661 ctggcgattt catcccgctc gccgaagaaa ccgggcagat agtcgccatg acgcagtgga
      721 tcatcgggcg cgcctgcgcg gacctggcgc gtctgcagaa ggagatcgcg cacggcgcgg
      781 ggttgcgcgt gtcggtcaac gtgtcgagcc gctatctgaa tcacggcaac gtcgtcgaag
      841 acgtccgcaa cggactcgcg gcgcaacggc tttcgcccga ctgcctgatc ctcgaagtca
      901 ccgagagcct gctgctggag aattccgcgc gcatcgaagc gatattccag gagctcaagc
      961 ggatcggcgt gcgcctcgcg ctcgacgact tcggcacggg ctattcgtcg ctggcctatc
     1021 tgcatcgttt tccgatcgac atcctcaaga tcgatcgctc gttcgtcgaa cgcctggcgg
     1081 gcgaggccga aggcgccggt ggagtcgacg ccgtggcgct ggcgcgcgcg atcctctcgc
     1141 tggccgatgc gctcggcctc gacaccgtgg cggaaggcat cgaacacgat gcgcaacgcg
     1201 acactttgtt agagctgggt tgcaagaccg gccagggtta ttcgttcggc aaggccatgc
     1261 cggtcggcga agtgctggat gccgcggtga ctcgccggcg cagcctgctg gcgggcaacg
     1321 ttcccggcca gatcgagtac tcggcgaccg gacggtttcg cggaatgacg gaaagcggct
     1381 gataccggtt tccgcgcgcc gaattccaca ttacataact cccagaatgg ttctcataat
     1441 cggccaactg aagagccggg ccgccgaatg gccgaaaacc tgagacctgc gtggcctttc
     1501 gcctaatggg cctcgactac tctgcatcgt cgcgagagac gtcagaaata gaacaatgca
     1561 gcggagtagc cggtgaagtc ggtagaaacg agatcgctcg acgtgggcgc actgcccgaa
     1621 cggagcactg aaagcgtttt gcgcgcggtg tgtctgcgcc tgggatgcac ggcggccacg
     1681 gcggtttttc cgcgcgagga ttcgcgcgtc gtgtggggac gctccgaaga ggatgtggcg
     1741 ttcgcgcgcg agcagctatc gatcgtcggc gacgaactgg cgcgccgcgc catcgaaacg
     1801 aagaaacccg ttgccaccaa caaactcaag cacgtgagcg gcgggcagat tgcctgcaag
     1861 ctggtcgcgg tgccgctcat cgcgcgtggc ggaccggccg gcgcactcat catcttcaat
     1921 cgcgcggaag aaccgagctt cgacgagttc accgtgcgcc gcctcggacg cttcgcgcgc
     1981 ctgctggcgc ggaatgcaac ccaggatctc gattcgctga cgggtctcct gtcgtggagc
     2041 ggtttcaagt cgcgcgtgga caactggcag cgcaatgcgc cgccggacgc catggtgtcg
     2101 atgctgtacg gcgacatcga tcgcctgcat ctcatcaacg atctcgcggg attctccgat
     2161 ggcgatcgcg tgatcgcgcg ttgcggtgtc gcgatccggc gcgcgctggt aggcggcgag
     2221 agtttcgcct gccgtctttc gggcgaccgc ttcacggtgt tcctgccgca catgaatctt
     2281 gcgcaggcgc ggttgctcgc cgaccgcatc tgcaaggaag tcagcacggc cgccgccgcg
     2341 aagaacgagc ctaaccaccc ggtttccatg tcgtggggcg cggtgtcgac cggcgtcggc
     2401 gatctgcatc tcgatcatcg catcgccgag gcggagatcg cctgcaagac cgccaaggat
     2461 cgcggccgtg gccgtgtcga ggtctaccag gacaccgacc agagcatggt ccgccgtcac
     2521 gacgagatcg gcctcatcgg cgcggtgcgc gaagcgctgc ggctcgagcg catcgtggtg
     2581 tacgcacagc ccatcacgcc gctgatcaat cacagcctgc cgacgacgta cgaactgctg
     2641 gttcgcatcg tcgacgcgaa aggcaacatc gtcgaagcgg ccgacttcat gtcggcggcg
     2701 acgcggtatc agattcttcc ggagatcgac cgcgcggtgt gcgagcgcgc gttcgaacag
     2761 ctcagcagga acacgtcgtc ggccggtcca ttgcgcgtgt cgatcaattt gtcggggccg
     2821 acgttgtcgg atccggattt cctcgagtgg ctgctggcgt cgatggccaa acatggcatc
     2881 gatggcacct gcctcgtgtt cgagatcacc gaggccgcgg ccgccgccaa cctgagtcac
     2941 gtgcagcagt tcatccgccg catgacgagt cacggtgtgc gattcgcgct cgatgacttc
     3001 ggcacgggcg tgagttcgct ggcctatctc aagaatctcg agatcaatac catcaagctc
     3061 gacggttcgt atgtcgtcga tgtcatgacc aacccgcggt cgcaggcgct ggtgcgcgcg
     3121 atcgtgcagc tggccgaggc catgggtatc accaccgtgg ccgagtacgt ggaatcgacc
     3181 atcgtgcgcg accgcctcgc gtcgctgggt attcacttcg ggcaggggta tgcactcggc
     3241 cgtccgggcc cgctcgatga cgtgctggcc gcggttgcgg accgcgcgcc ggaagggcgt
     3301 gaccggccgt tcacggacaa caaggcagcg ttcaccccgt cgggtatccc gatcatgttc
     3361 aagcacgcgt aaggaaatgg ggccagaccc catttgctag gaaattgggc cagacctgag
     3421 ctttcgtggc cggccaaccg agcagagtgt ttgttccagg ccagccgtgc gcgcctgcga
     3481 aaccgtccag tctgaatcgc catacggccg ctgacgattg acgc
//
LOCUS       DATDEU010000040         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_152688, whole genome shotgun sequence.
ACCESSION   DATDEU010000040 DATDEU010000000
VERSION     DATDEU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152688"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VIV63_00835"
     CDS             <1..317
                     /locus_tag="VIV63_00835"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6123167.1"
                     /translation="GKLEPDEPPDITVQRELVEEGGVSAATWESLGNFFSSPGVFTEV
                     IHLYLATDLTPAVSAPEAGEVFEVEWWPLEIAVEKARNGELTDGKTIIGILRAASHRK
                     FA"
     gene            402..767
                     /locus_tag="VIV63_00840"
     CDS             402..767
                     /locus_tag="VIV63_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123168.1"
                     /translation="MSDNNSNGKPPQAANRRRVSQIVHDERGNARVEWIDAGRAGVPI
                     ERAPLSVEQTPSRGDNAKLAVERNRASGFDPYARVGAAHIPEPKKPPVKRDLRKLGEW
                     IKLKRELDERKSQDDTDVE"
     gene            complement(783..1439)
                     /locus_tag="VIV63_00845"
     CDS             complement(783..1439)
                     /locus_tag="VIV63_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123169.1"
                     /translation="MSHATLASLQSALTEIYDLPATPDVREFLMTNRAAIGAGHERAS
                     DEQLIVAEDGDTLSMALYIDGAVLERLGGRDPISELTQDNLADYLTVAEGVSHFVYVA
                     WNTGFDKPVTLLELELQAEVDKYVLCAWLLREQGSGRFPHELHRVLFERARIDAVAAA
                     GRIGLYHLASTYAARFCRRVATLLERKRHGVTRDVMAELRRFYRWGNVRKLRHIEKYA
                     "
     gene            complement(1441..2073)
                     /locus_tag="VIV63_00850"
     CDS             complement(1441..2073)
                     /locus_tag="VIV63_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123170.1"
                     /translation="MTIQPVASLKEFFRDALHDALSHQRVAVEGETEHYVVNVLTLFS
                     DADALYDRHSQHESRPPDSRKHLKPLAMMLGEALEAPTNEARFRGLQRLGDVSLFIAG
                     FFSAGFARKLVDIDYHIAMGGQAYGALAGSCPSARAKTMRQVFAELAAKFVPMVDALN
                     EISETAYQHSDQDRLRLYELWVKTGSERSRQLLRKLGLEPVPAARSVLAH"
     gene            complement(2250..2948)
                     /locus_tag="VIV63_00855"
     CDS             complement(2250..2948)
                     /locus_tag="VIV63_00855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY6123171.1"
                     /translation="MPIGAIRAICFDLDNTLWEVEPVLKRAERILADWLERRYPLIPQ
                     RFSSAAVLEMRAALLAEQPHQAHDLSFLRRETLARCAAEVGYKRDMAHEAFAHWLAAR
                     NQVEPYAEVIAALETLKARYRLATLSNGNADLAVIGLAHHFEVTLSAGALGCAKPDPR
                     TYAALADALTLKPAEILFVGDEPHADVVGPRAAGMQTVWVNRGGVVWDDALPAPDASV
                     TDLAGLQALLSEKL"
BASE COUNT          560 a          979 c          978 g          532 t
ORIGIN      
        1 cgggcaagct cgagcccgac gaaccgcccg acatcaccgt ccagcgtgaa ctcgtggaag
       61 aaggcggcgt gtcggcggcc acctgggaat cgctcggtaa ctttttcagc tcgcccgggg
      121 tattcaccga agtcatccat ctctacctgg cgaccgatct gactcccgcc gtgtccgcgc
      181 cggaagccgg tgaagttttc gaggtcgaat ggtggcccct cgaaatcgcc gtggagaagg
      241 cccgcaatgg cgaactcacg gacggcaaaa cgatcatcgg aattttgcgg gccgcgtcac
      301 accgaaaatt tgcgtgaaac gcttcacagg ccgcgattcc ggagtgccgg aatggtcgct
      361 atgtcaaaca acgaaaacgg cgcgtcagtc atacttgcgc cgtgtcagat aacaattcaa
      421 acggcaaacc cccacaggcc gccaaccggc gccgtgtgtc gcagatcgtg cacgacgaac
      481 gtggtaacgc gcgtgtcgag tggatcgatg ccggacgcgc cggcgtaccg atcgagcgtg
      541 ctccgctgag cgtcgagcag accccgtctc gcggcgacaa cgccaaactc gccgtggaac
      601 gaaatcgcgc gagtggtttc gatccctacg cccgcgtcgg cgccgcgcac attcccgagc
      661 ccaagaagcc cccggtcaag cgcgacctgc gcaagctcgg cgaatggatc aagctcaagc
      721 gcgaactcga tgagcgcaag tcgcaggacg acaccgacgt cgagtgagct tctccgccgg
      781 cgtcaggcat atttctcgat gtgccgcagc ttgcgcacgt ttccccagcg atagaaccgc
      841 cgcagctcgg ccatgacgtc acgcgtgacg ccatgccgtt tgcgctcgag cagcgtggcc
      901 acgcgccggc agaagcgcgc ggcataggtc gacgccaggt ggtacaggcc gatgcggccg
      961 gcggcggcca ccgcgtcgat gcgcgcgcgc tcgaacaaca cccggtgaag ctcgtgcgga
     1021 aagcgcccgc tgccttgttc gcgcagcagc cacgcgcaca acacgtactt gtcgacttcg
     1081 gcctgcagtt cgagctcgag cagcgtgacc ggcttgtcga agccggtatt ccacgcgacg
     1141 tagacgaagt gactgacacc ttcggccacg gtgaggtagt cggcgaggtt gtcctgggta
     1201 agctcgctga tcggatcgcg gcccccgagc ctttcgagta cggcgccgtc gatgtacagc
     1261 gccatagaca gcgtgtcgcc gtcttccgcc acgatgagct gctcgtcgct ggcacgctca
     1321 tggccggcgc cgatcgcggc ccgattggtc atcaggaatt cgcgcacgtc cggagtcgcc
     1381 ggcagatcgt agatttcggt gagcgcactc tgcagcgacg cgagagtggc gtggctcatg
     1441 tcagtgcgcc agcacgcttc gcgcggcggg cacgggctcg agcccgagtt tgcgcagcaa
     1501 ctgccgcgag cgctcgctgc cggtcttgac ccacaactcg tacaggcgca gccggtcctg
     1561 atccgaatgc tggtaggccg tttcgctgat ctcgttgagc gcgtcgacca tcggcacgaa
     1621 cttcgcggcg agctcggcga agacctgcct catcgttttt gcgcgcgccg acgggcaact
     1681 cccggcgagc gcaccgtagg cctgcccgcc catcgcgatg tggtagtcga tgtcgaccag
     1741 cttcctggcg aatccggcgg agaagaaccc ggcgatgaac agcgagacat cgccgagacg
     1801 ctgcagtccg cggaacctgg cttcgttggt cggcgcctcg agcgcttcgc ctaacatcat
     1861 cgccagcggc ttgaggtgct tgcgcgaatc gggcgggcgt gactcgtgtt gtgaatggcg
     1921 gtcgtagagg gcgtcggcgt cggagaacag cgtcagcacg ttcaccacgt agtgttcggt
     1981 ctcgccttcg accgccacgc gttgatgcga cagcgcgtcg tgcaacgcgt cgcggaagaa
     2041 ttccttaagg ctcgcaaccg gctggatggt catctttcac cccgatcgat tgaatgcact
     2101 caatatcatc ggcgccagac gactttcagt tgagcgcgtt cccgacgcgg gttccgtgac
     2161 gcaagagccg attccacgaa tccgcgcacc cgcccggctg gcagccgctg atgcacactg
     2221 ctaactacgt ccggaccacg gtgcacggtt cacagttttt cgctgagaag tgcctgaagt
     2281 ccggcgaggt cggtgacgct ggcatccggc gcgggcaacg cgtcgtccca aacaacacca
     2341 cctctattca cccatacagt ttgcatgcca gcggcgcggg gaccaacgac gtcagcgtga
     2401 ggttcgtcgc ctacaaacag tatttccgcg ggtttaagtg tcagtgcgtc ggccagcgcg
     2461 gcgtaggtgc gcgggtccgg cttggcacat cccagtgcgc cggcgctcaa agtgacttca
     2521 aaatggtgcg cgagtccgat gaccgcgagg tcggcgttgc cgttgctcaa cgtggcgagc
     2581 cggtaacgcg ccttgagggt ctcgagcgcg gcaatgacct cggcgtacgg ttcgacctgg
     2641 ttgcgtgccg caagccagtg cgcgaacgct tcgtgggcca tgtcccgctt gtagcccacc
     2701 tccgccgcgc agcgcgccag cgtctcgcgc cgcagaaacg acaggtcgtg cgcctggtgc
     2761 ggttgttcgg cgaggagcgc ggcgcgcatc tcgagcaccg cggccgacga aaaccgttgc
     2821 ggtatgagcg ggtatcggcg ttcgagccaa tcggcgagaa tgcgctccgc gcgcttcaat
     2881 acgggctcga cttcccagag cgtgttgtcg aggtcgaagc agatcgcgcg aatcgcgcca
     2941 ataggcatgg cgtgcatgtt aacgggggga accgcttgga cgaaatgctg ctcaccatgg
     3001 gcaacaagaa ctactcgtcg tggtcgctgc gcccgtggct gctcatgaa
//
LOCUS       DATDEU010000041         2364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2049818, whole genome shotgun sequence.
ACCESSION   DATDEU010000041 DATDEU010000000
VERSION     DATDEU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049818"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2348)
                     /locus_tag="VIV63_00860"
     CDS             complement(<1..2348)
                     /locus_tag="VIV63_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6123172.1"
                     /translation="MQVSVGSIGGLPATPGQAITASIIGPTRMSTAEEFRDILLRVNA
                     DGSQVRVGDVGRVGLNSESYIRDTKYNGKPAAGVAIRLAAGQNALDTMAAIHDTIDRL
                     APFFPRGLEVQYPLDTTPFVRTSILEVVKTLFEAVILVFLVMYLFLQNARATLIPTIA
                     VPVVLLGTFGILAAFGFSINTLTMFGMALSIGLLVDDAIVVVENVERVMAEEGLSPRE
                     ATRKSMDQITGALVAIALVLAAVFVPMAFFGGSIGVIYRQFSITLVSAMGLSVLVALI
                     LTPALCATILKPLPPGGHVAHRGFFGWFNRMFDNGRGKHERGLTAMLGKPARSMLAFL
                     AIVVVTGLLFTRIPTSFLPAEDPGFAYGQVQTPPGASKERTWAALDKAQEYLLNEEKD
                     VVEGVLTVNGFNFAGTGQNSGLIFIKLKDWDERTKDSQSMQAFLGRTNQFFSSITEAN
                     VIAVPPPAVLELGNTAGFDLMLQNRGGMPRADFLAARNQLLGEAAKNPVLTGVRPNGV
                     EDAPQFKLDIDREKASALGLSIADVNQTLQTGWASTYVNDFIDRGRVKRVYVQGDPQS
                     RMQPEDLNRWYVRNSQGAMVPFSAFGRGEWTYGPQKLLRFNGVPAFQIQGSPAPGHSS
                     GEAMDAMEDIVAKLPAGIGLEWNGLSFEEKKSGSQAPLLYVLSIAIVFLCLAALYESW
                     SIPATVLLVVPLGVLGAVLATLSRGLTNDAYFQVGLLVTVGLAAKNAILIVEFAKENF
                     DRGQDLVEAVMHAAKQRLRPIIMTSLAFMSGVLPLAISSGAGS"
BASE COUNT          423 a          748 c          793 g          400 t
ORIGIN      
        1 gaaccggcac cgctcgaaat cgcgagtggc aacacgcccg acatgaatgc cagcgaggtc
       61 atgatgatcg ggcgcaatcg ctgcttggcg gcgtgcatca ccgcctccac gagatcctgc
      121 cctcgatcga aattctcctt ggcgaattcc acgatgagga tggcgttctt cgcggccagg
      181 cccacggtca cgagcaggcc tacctggaaa tacgcgtcat tggtgagtcc gcgcgacagc
      241 gttgccagca ctgcgccgag cacgcccagt ggcaccacca gcagcacggt cgcgggaatc
      301 gaccagctct cgtacaacgc ggccaggcac aggaacacga tggcgatcga cagtacgtac
      361 agcaggggag cctgcgatcc ggatttcttt tcctcgaagg acaggccgtt ccactccagt
      421 ccgatgcccg ccggtagttt ggcgacgatg tcttccatgg cgtccatggc ttcaccggag
      481 ctgtgcccgg gcgccggaga accctggatc tggaacgccg gcacgccgtt gaagcgcagc
      541 agcttctgcg ggccgtaggt ccattcaccg cggccgaacg ccgagaacgg caccatggct
      601 ccctggctgt tgcgcacgta ccagcggttc aggtcttccg gctgcatgcg cgactgcgga
      661 tcaccctgca cgtacacgcg cttcacgcgg ccgcggtcga tgaagtcgtt cacgtaggtg
      721 gacgcccagc cggtctgcag tgtctggttc acgtcggcga tcgacaggcc cagcgcactc
      781 gccttttcgc gatcgatgtc gagcttgaac tgcggcgcgt cctcgacgcc gttgggccgc
      841 actccggtca gcaccgggtt cttcgcggcc tcgcctaaca actggttgcg cgcggcgagg
      901 aagtcggcgc gcggcatgcc accacgattc tgcagcatga gatcgaagcc ggcggtgttg
      961 ccgagctcga gcaccgcggg tggcggaacg gcgatgacgt tcgcttcggt gatgctcgag
     1021 aagaactggt tggtgcggcc gaggaacgcc tgcatggact gcgagtcctt cgtgcgctcg
     1081 tcccagtcct tgagcttgat gaagatgagt ccggagttct gaccggtgcc ggcgaagttg
     1141 aagccgttca cggtgagcac gccttcgacg acgtccttct cttcgttcaa caggtattcc
     1201 tgcgccttgt cgagcgcggc ccaggtgcgt tccttggacg cgccgggcgg cgtctgcacc
     1261 tggccgtagg cgaagccggg atcttcggcg ggcaggaacg acgtcggtat gcgcgtgaag
     1321 agcagtccgg tgaccacgac gatggcgagg aacgccagca tcgagcgcgc cggcttgccg
     1381 agcatcgccg tcaggccgcg ttcgtgtttg ccgcggccgt tgtcgaacat gcggttgaac
     1441 cagccgaaga atccgcgatg cgccacgtga ccacccggcg gcagcggctt gaggatggtg
     1501 gcgcacagcg ccggtgtgag gatgagtgcc accagcaccg acaggcccat ggccgacacc
     1561 agcgtgatgg agaactggcg atagatgacg ccgatggagc cgccgaagaa cgccatgggc
     1621 acgaacaccg cggccagcac cagcgcgatg gcgaccagcg cgccggtgat ctgatccatg
     1681 gacttgcgcg tggcttcacg cgggctaagg ccctcttccg ccatgacgcg ctcgacgttc
     1741 tccacgacga cgatggcatc gtccaccagc aggccgatgg acagcgccat gccgaacatg
     1801 gtcagcgtgt tgatcgagaa tccgaaggcc gcgaggatgc cgaacgtgcc taacaacacc
     1861 acgggcacgg cgatggtcgg aatgagcgtg gcgcgtgcgt tctgcaggaa caggtacatg
     1921 accaggaaca cgaggatcac ggcctcgaac agcgtcttca ccacttcgag gatggaggtg
     1981 cgcacgaacg gcgtggtgtc gagcgggtac tgcacttcga gaccgcgcgg gaagaacggc
     2041 gcgagccggt cgatggtgtc gtggatggcg gccatcgtgt cgagcgcgtt ctgtccggcc
     2101 gcgaggcgga tggcgacacc ggccgcgggt ttgccgttgt acttggtgtc gcggatgtaa
     2161 ctttcggagt tgagcccgac gcggcccacg tccccgacgc gcacctgcga gccgtcggca
     2221 ttgacgcgca acaggatgtc gcggaattcc tcggccgtgg acatgcgcgt gggaccgatg
     2281 atgctggcgg tgatcgcctg tcccggcgtc gccggcaaac ccccgatgga gcccaccgac
     2341 acctgcacgt tctgcgcgcg gatc
//
LOCUS       DATDEU010000042        11838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1903960, whole genome shotgun sequence.
ACCESSION   DATDEU010000042 DATDEU010000000
VERSION     DATDEU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903960"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VIV63_00865"
     CDS             complement(<1..1166)
                     /locus_tag="VIV63_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY6123173.1"
                     /translation="MNKLLPAAFLASLVATVALGAPAFNPVGDVSGRYSPVTVSPGAI
                     PLYFPSGSTAGPDAPTDMKGQAAVTLQRLSANLKDAGLSLEDVAFARAYLVPGADGKV
                     DYEGWNAAWDEVFNNPAKPVAVKPARTTVAVPLLGRVGTLIEIDFICLPKKGTDFASA
                     ARSGLPAFNSQLKPYGTKERHILAGIGVLPNTALYFTQGTTPPVLDEAVPATDIRHRG
                     DMRTQARNTLLALQKNLAGVGLTFKDVVYLRAFLAPDQRTKAFDYDGWNAAYGEFFNN
                     AASNPHKPARTTVTTPTYGSPSMMIEIEIIAAFPKAPDLFDSKADNPRLKAYGEPTAM
                     ISSGVAVAANAPLVLSAGVVSAVPGDMKTQALSALDTLQKRLTAAGLTFKDVVFL"
     gene            complement(1163..3340)
                     /locus_tag="VIV63_00870"
     CDS             complement(1163..3340)
                     /locus_tag="VIV63_00870"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY6123174.1"
                     /translation="MSQSRLWKYAMRASLASIAAIAATATLIDATAWSHGNEARMVEF
                     LNWKKMPNIARVFGANRAHLEGTPSGSVRPQIRMVEGQSCIVGQVIGFDVDDRYAFDI
                     DEPVELSVTYATAYTSPFVIGWDKSGGSGAGVIEITPAPGETFTTAKVTLDRARLAGQ
                     ATQGADIAIGAPNGIVVCSIEVVRSNKTIVPEAYGRVKLTLRDAKTGGLVPARLGLYD
                     KTGRAPLASDKSLMLQRFADDLRMLAANERTFWPSENRQVFYADGNYETRVPVGTYEL
                     VASRGIEYKFHRSQIEVTKDKTTEVTIDLQRYADMPAAGWYSGDAHIHVTRDEVADPQ
                     LWGFVSAEDVHVGNLLEMGNVQNVYFHQPKAWGKASRFERDGHFIVSGQESPRTGQFG
                     HTIHFNIQRPVHLKTDEYFLYHKVFQEVASQPGGISGFAHMGWRGAGEQGNRTGQMNR
                     GMALLAPLGLVDFIEVLQGGRLVNEGWYRLLNLGYRVKPAAGTDWPYSDFPGVVRFYV
                     KVDGPFNLDSWFASYDKGRTFVTNGPLLDFTINGKGIGEELRVKRGTRLDVAAAARLN
                     PQLDKLDRLELVVLGDVDATQSADGKESVSLRKELTAEHSMWVAVRAFGARQDPRNTT
                     IAHSAPIYVVVDDEPTWKREAVPEIVAELRGRVQRILTDPIDTPISGNEVWETRLTLQ
                     DQWLLQQPLLKPTVDAADAAYQKLLDRHARFAAPAPATVGSTR"
     gene            complement(3402..4502)
                     /locus_tag="VIV63_00875"
     CDS             complement(3402..4502)
                     /locus_tag="VIV63_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY6123175.1"
                     /translation="MPENFHLETLRFAEPQFLWLLWVPAGLCLLLVWRVFRRRRDLAK
                     CAIALPAPLRARPGLLGDLAPWLPVVLAAAACIVALAQPQALAAVIDKSGVDLVILQD
                     ASASMHVEDVPPNRWQRSQQFVRVMAASLRWEGDRAALALFAHRTAPQLRLTRDPNSL
                     LFFLDHLGEVPPFPLEDDTTWNTNIAEAVLWGLKLVEKNEEMFGRSNNARAFLLISDG
                     QAWSGELDAALAQARARDVKVYVVGVGTVTGGLIPQPRGDDGQLPDQKIRGTLDRAAL
                     RYIARQGGGQYFELGVAPDRDTAFSILAAARQHASGSQVEERVEPLYWYALLAAVLSA
                     CLAGALASRTVERRWLLGGAVVTLCVLVQRVL"
     gene            complement(4502..5197)
                     /locus_tag="VIV63_00880"
     CDS             complement(4502..5197)
                     /locus_tag="VIV63_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123176.1"
                     /translation="MRAKPIYLFLACLLFIAGGAGVYWGKLQSRLAQTQLSLVTQQHA
                     GVRETLTELAQLHRFAAYLPGGRATLAQIRADLAQLSYWQRDYRALIPQQGDPMAELP
                     KDNLRLQSIVANAVFREKYPPAKDQKSRLEALDAAIAAQLAVLRNSPDGTGVAYNYEY
                     LVRLRGLAAGKSGPPKAGAGGDHGALGHLGGEPQAADPTDFKIHIPLESRELQDQKEG
                     REAGKTAPRERKG"
     gene            complement(5194..6267)
                     /locus_tag="VIV63_00885"
     CDS             complement(5194..6267)
                     /locus_tag="VIV63_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY6123177.1"
                     /translation="MIAEVLWEWLQLRPEELRFGRMEAAILVATGLSMAAALLIAWHA
                     RRQQQRRKPHAIALAALLPVVRPSAGAAMRHAPFVLFVAGLGFALLALADPFTTLVRE
                     DKTFPGRRIAVLIDASSSMNQPFHSPSFNKQGGPTYFATVAAAEYFMRLRMEGTFRDL
                     IALVEFGNEAYVVTPFTTDYENVLLSMRLIAEPAEWERFPDQGTIIMRAIQKSTELFE
                     TFDFLGAAGNLIVIFSDGQDSQTILQGRPIKHIMADARAHGIPVYMIRVAFNKGLGAV
                     MPDELWKEAVEQTGGRFYPAADEATILRAVHEIDQLAPGKVELREYRSRELQFPGYAL
                     IAVLLWLAATAAKLGLRTFRTFP"
     gene            complement(6270..7412)
                     /locus_tag="VIV63_00890"
     CDS             complement(6270..7412)
                     /locus_tag="VIV63_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123178.1"
                     /translation="MRRCLTLLMLLAGASASVGADAEAEVQPLQCGWRTDRVAVHVGE
                     PLRLTLTCAAAETDAATVMPDWDQLQPDVVDLAPFEVVAGTRSEDVVTPPWRYTQFEY
                     TLRLTGEEFFGLDVPLPPLEIAYSIAVGPRGGNVQMGRERTYVLPPLPMKILALVPAR
                     ASDIEDVTAHSFVSIERRRSQASMVFLVSCGLLAAGALYLLLFLGSAWRAMRTRRVKP
                     AFRIPDWRLVLGARAALRKQVSAAGKQGWTDARVGEALAVLRVLGAVAAGLPVRQQVA
                     ARDRPAGNGEWLLRRLSLRRPYVAISATVTAAQLRAHASLEELAVAIAVCSDVRYGPE
                     IADGGMPEVGRAIQDALGASRELLVASLLPSRLQFLAGKRVATGAA"
     gene            complement(7413..8336)
                     /locus_tag="VIV63_00895"
     CDS             complement(7413..8336)
                     /locus_tag="VIV63_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY6123179.1"
                     /translation="MNPAAREPGWLINPADVSAIETFLLRRMREHASGDHASVFAGNG
                     FDVRGLREWEPGDGMSSIDWAQSSINNFSPLITRELEQDSAATVMVAADASLSTHCGP
                     AGAQLRTTILRSLAVLGLSAAFSQDRFGIVAFDDLCRPLATLRPRAGKAHVMHCLSLY
                     AQCSDDRKAGSGELIPQLESYLRGSSLLVLVSDCLLTGIDELLERFAVLTGEHDVLVV
                     MVDARPVFAVPPVSAGWLEIHDVETGESRTVSRAAAAAMIERIDEWQTAMVGHARSRG
                     LDIVRVGAGRWELEETLSACFASRRLQKMRG"
     gene            complement(8333..9373)
                     /locus_tag="VIV63_00900"
     CDS             complement(8333..9373)
                     /locus_tag="VIV63_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6123180.1"
                     /translation="MHADLTEAIAFHEAVLAEGGKALIGYEKEKALANIVLMAEIPTS
                     YDKGQKRQVNAGNLLLRGVPGVGKTFFGVILAAISDAKFARIQGRADLHPAEVVGFQM
                     INPATGEFVTEFGPLASAEIILLDEINRIPVKSQSAFLEALQDRTVTVGKTTCDLPEF
                     SFALATMNPIELGQGTFALSEAATDRFAIIVNVPYLPPQEEQKLVSFDFKKVSLKPLA
                     TKDRILELRAAISREVFLHERIAAYIQRLVAATRPLDAASGWTQRSPSALVESYVELG
                     ASPRATICWGRLVKVWAVLQRKRSFVLPEDVQELARYVLGHRVWLAPQAASAGITVEA
                     VIDDVVHQVAVP"
     gene            complement(9375..11486)
                     /locus_tag="VIV63_00905"
     CDS             complement(9375..11486)
                     /locus_tag="VIV63_00905"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY6123181.1"
                     /translation="MGRVAFACLTALLSVASFGHGTEGRYLEILNWKKPNVARTFGSN
                     RAHLDGIATTSNPVQARVIAGQGCVVGDLLLFDVDNAFAFDIDETVTLTLTYASDYSS
                     PFIVAWDRNGGTGQGKSAEIVPQPAAGFQKQKLVLERARFAGQATQGADIAIGTRSGV
                     AICDITLERSNSTPLPAAFGEALLTIKDGPTGRRVPARVGIYDATGRAPLASDDSLKL
                     QRFADDLRMLAVNERTFWPSDHRQAFYVDGEYRARLPAGQYELVVTRGPEFRAHRSSF
                     EIKSGATSRLDVSLQRYANLPAKGWYSGDAHVHVTRDQVADPQIWGFVAAEDVYVGNL
                     LEMGNIAKLHFHQPQAWGKASRFERDGHFIVSGQEDPRTGHFGHTIHHNIQSPIRAPS
                     DSYFLYHKVFQESARQGGISGFAHMGWNQAGSTGAGQIAPQGQFNRGLAILAPTGLVN
                     FIEVLQRGRLIHEGWYRLLNLGYRINPAAGSDWPYSDFPGVVRNFVKLDGPLNLDAWF
                     AAFRAGHTYVTNGPFLEFSINGRGMGQELKVKRGTVLDITAATQLNPDVDALDRLELI
                     VLGEVAASAPAKGADNAGIRHQLVADRSMWIAVRSYGGRQHPDNMTIAHSTPIYVVVD
                     DEPTWNRAALPTIVSELRQQLQRIMVERIEPQTGPEPWETRTVLAEEWLLQRPLLKPH
                     VDAADAAYEQLLISLQKYTAQ"
     gene            complement(11500..>11838)
                     /locus_tag="VIV63_00910"
     CDS             complement(11500..>11838)
                     /locus_tag="VIV63_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123182.1"
                     /translation="AEAALKKLAAMADRGEYYFKLGAMYGDNEQWSDSRQALERAVQK
                     GGLKRPGEAWMRIAVANYNLSNVPGTQAALQQAMKFDESRKQAGEWLKYLGNAPAPKP
                     AGDQKLAKAD"
BASE COUNT         1999 a         3969 c         3825 g         2045 t
ORIGIN      
        1 aggaacacga catccttgaa tgtcaggccg gcggcggtga gccgcttctg caaggtgtcg
       61 agcgcggaca acgcctgggt cttcatgtcg ccgggcacgg cggagaccac acccgccgac
      121 aacacgagcg gcgcgttcgc ggcgaccgcc acgccggaag agatcatggc ggtcggctca
      181 ccgtaggcct tcaggcgcgg gttgtcggct ttcgaatcga acagatcggg tgccttggga
      241 aaggccgcga tgatctcgat ctcgatcatc atgctcggcg atccgtacgt cggtgtggtc
      301 accgtcgtgc gcgcgggttt gtgcggatta ctcgccgcgt tgttgaagaa ctctccgtag
      361 gcggcattcc agccgtcgta gtcgaaggct ttggtgcgct ggtccggcgc gagaaatgcg
      421 cgtagataga ccacgtcctt gaacgtgagt cccacgcccg cgaggttctt ctgcagcgcc
      481 agcaaggtgt tacgcgcctg cgtgcgcatg tcgccgcgat ggcggatgtc cgtggccggc
      541 accgcctcgt cgagcaccgg cggcgtcgtg ccttgcgtga agtacaaagc cgtgttcggc
      601 aacacgccga tacccgcgag aatgtgccgc tccttcgtgc cgtagggctt cagttgcgaa
      661 ttgaaggcgg gcaatcccga acgcgcggca ctcgcgaaat ccgtaccctt cttcggcagg
      721 cagatgaaat cgatttcgat cagcgtgccc acccgtccca gcaagggcac ggccaccgtg
      781 gtgcgggccg gtttcactgc gaccggcttc gcgggattgt tgaagacttc gtcccaggcc
      841 gcgttccagc cttcgtagtc cactttgccg tcggcgccgg gtacgaggta cgcacgtgcg
      901 aacgcgacat cttcgagcga aagccccgcg tccttgagat tggcgctcag gcgctgcagc
      961 gtcacggccg cctgaccctt catgtccgtg ggcgcatcgg ggcccgccgt cgaccccgag
     1021 gggaagtaca gcgggatcgc gcctggcgaa acggtcaccg gcgagtaacg tccgctgacg
     1081 tcacccaccg gattgaaagc cggcgccccc agcgcgacgg tggcaacgag cgaagccagg
     1141 aacgccgccg gcagtagttt gttcatcggg tggaacccac ggtggccggc gccggtgcgg
     1201 caaacctggc atgccggtcg agcagcttct ggtacgccgc atccgcggca tcgacggtcg
     1261 gcttgagcag tggctgttgc agcagccact ggtcctgcag cgtgaggcgc gtctcccaca
     1321 cttcgttgcc ggaaatcggc gtgtcgatgg gatcggtgag gatgcgctgc acgcggccgc
     1381 gcaactcggc gacgatttcg ggcacggcct cgcgtttcca ggtcggctca tcgtcgacca
     1441 ctacgtagat aggcgcggag tgggcgatgg tggtgttgcg cgggtcctga cgggcgccaa
     1501 acgcgcgcac cgcgacccac atgctgtgct ccgcggtcag ttccttgcgc agcgagacgc
     1561 tctccttgcc gtcggccgat tgcgtcgcat cgacgtcgcc gagcaccacg agctcgaggc
     1621 gatcgagctt gtcgagctgc ggattgagac gcgcggcggc ggccacgtcg aggcgtgtgc
     1681 cgcgcttcac gcgcagctct tcgccgatgc ccttgccgtt gatggtgaaa tcgagcagcg
     1741 gcccgttggt gacgaaggtg cgacccttgt cgtaagacgc gaaccagctg tcgagattga
     1801 agggtccatc gaccttgacg tagaagcgca ccacgccggg gaaatcgctg tacggccagt
     1861 cggtgcccgc cgccggcttg acccggtagc cgagattgag cagccggtac cagccctcgt
     1921 tcacgaggcg gccgccctgc aacacttcga tgaagtccac gagaccgagt ggcgcgagca
     1981 acgccatgcc acggttcatc tgacccgtgc ggttgccctg ctcgcccgct ccgcgccatc
     2041 ccatgtgcgc gaagccggag atgccgccgg gctggctagc tacttcctgg aataccttgt
     2101 gatacaggaa gtactcatcg gtcttcaggt gtaccgggcg ctggatgttg aagtgaatgg
     2161 tgtggccgaa ctggccggtg cgcggcgact cctgcccgga cacgatgaag tgaccgtcgc
     2221 gttcgaaccg gctcgccttg ccccaggcct tcggctggtg gaagtagacg ttctggacgt
     2281 tgcccatctc cagcagattg ccgacgtgca cgtcttccgc cgagacgaaa ccccagagct
     2341 gcggatcggc cacttcatcg cgcgtcacgt ggatgtgcgc atcgcctgaa taccagccgg
     2401 ccgccggcat gtcggcgtag cgctgcaggt cgatcgtcac ctccgtggtc ttatccttcg
     2461 tgacctcgat ctggctgcga tgaaatttgt actcgatgcc gcggctcgcc acgagttcgt
     2521 aggtgcccac ggggacgcgg gtttcgtaat tgccatcggc gtagaacacc tggcggttct
     2581 cgctcggcca gaaggttctt tcgttggcgg cgagcatgcg aaggtcgtcg gcgaaccgct
     2641 gcagcatgag cgacttgtcg ctggccagcg gtgcgcgtcc ggtcttgtcg tacaaaccga
     2701 gtcgcgcggg aaccagcccg ccggtcttcg cgtcgcgcag cgtgagcttc acgcggccgt
     2761 aggcttcggg gacgatcgtc ttgttgctgc gaaccacttc gatgctgcac acgacgatgc
     2821 cgttcggcgc accgatcgca atgtccgcgc cctgcgtcgc ctgccccgcc agtcgcgcac
     2881 ggtccagcgt cactttcgcc gtcgtgaatg tctcgcccgg cgcaggcgtg atctcgatga
     2941 cgcccgcgcc gctgccgccg ctcttgtccc agccgatgac gaacggcgag gtatacgccg
     3001 tcgcatacgt cacggagagt tcgaccggtt cgtcgatgtc gaacgcgtag cggtcgtcga
     3061 cgtcgaagcc gatcacctgg ccgacgatgc acgactgccc ctcgaccatg cggatctgcg
     3121 gccgcacgga gccgctcggt gtgccttcga ggtgggcgcg gttcgcgccg aagacgcgcg
     3181 cgatgttggg catcttcttc cagttcagga actcgaccat gcgggcttcg ttgccgtgac
     3241 tccacgccgt cgcgtcgatc agcgttgcag tcgccgcaat cgccgcgatc gaagcaagcg
     3301 acgcgcgcat ggcgtacttc cacagtctgc tctggctcat cgctgtctcc ggttggttac
     3361 gtatcggaga cggacggaat gctagggcac cctacctcgt gtcacagcac ccgctggacc
     3421 agcacgcaga gcgtcacgac ggcgccgccc agcagccagc gtcgctcgac tgtgcggctc
     3481 gcgagcgccc cggcgagaca ggcactcagc accgccgcta acaaggcgta ccagtagagt
     3541 ggctcgacac gttcttcgac ctgcgaaccc gaggcgtgtt gccgcgcggc ggcgagaatg
     3601 gaaaacgccg tgtcccgatc cggtgcaacg cccagctcga agtactgacc gccgccctgc
     3661 cgcgcgatgt agcgcagcgc cgcgcgatca agcgtcccgc ggattttctg gtcgggcagc
     3721 tggccgtcat cgccgcgcgg ctgcgggatc agcccgcccg tcaccgtccc gacgccgacc
     3781 acgtagacct tcacatcgcg cgcccgcgct tgcgccaggg cggcgtccag ctcaccgctc
     3841 caggcctggc catcggagat cagcaggaac gcacgcgcat tgttgctgcg gccaaacatc
     3901 tcctcgttct tctcgaccag cttcaacccc cacaacacgg cctcggcaat gttggtattc
     3961 caggtcgtgt cgtcctcgag cgggaacggc ggcacttcac ccaggtgatc gagaaagaac
     4021 agcagggaat tcgggtcgcg cgtcaatcgc agctgcggcg ccgtgcggtg cgcgaacagc
     4081 gcgagcgccg cgcgatcgcc ctcccagcgc agcgaggccg ccatcacccg cacgaactgt
     4141 tgcgagcgtt gccagcgatt cggcggcaca tcctcgacat gcatcgaggc ggatgcatcc
     4201 tggaggatga ccaggtccac gccactcttg tcgatcacgg cggccagcgc ctgcggctgg
     4261 gccaacgcca cgatacaggc cgcggccgcc agcacgacgg ggagccacgg ggccaggtca
     4321 cctaacaatc ccggtcgggc cctcaggggc gcgggcagtg caatggcgca tttcgccaga
     4381 tcccggcgac gccgaaagac gcgccacacc agcagcaggc acagccccgc cggcacccac
     4441 agcaaccaca ggaactgggg ctccgcgaat cgcagggtct cgaggtgaaa gttttccggc
     4501 atcagccttt gcgttcgcgc ggcgcagtct tgccagcttc gcgcccctcc ttctggtcct
     4561 gcagttcgcg tgactcgagc gggatgtgaa tcttgaagtc cgtgggatcc gcagcctgtg
     4621 gctcgccgcc caggtgaccc agtgcgccgt ggtcgccgcc cgcacccgcc ttgggcgggc
     4681 cactcttgcc ggccgcaaga ccgcgcagcc ggaccaggta ctcgtagttg taggcaacgc
     4741 cggttccgtc cggcgagttg cgcagcacgg cgagctgcgc ggcgatcgcg gcatccagcg
     4801 cttcgagccg cgacttctga tccttcgcag gcgggtactt ctcgcggaac acggcattgg
     4861 cgacgatcga ctgcaggcgc agattgtcct tgggcagctc cgccatcggg tcgccctgct
     4921 gcgggatcag cgcacgatag tcgcgttgcc agtagctgag ctgtgccaga tcggcacgga
     4981 tctgcgcgag agtcgcgcgc cctcccggca ggtacgctgc gaaccgatgc agttgcgcaa
     5041 gctccgtcag tgtctcgcgc acgcccgcgt gctgctgcgt gacgagggac aactgggtct
     5101 gcgcaagccg cgactgaagt tttccccagt acacccccgc gccaccggcg atgaacagca
     5161 ggcacgccag gaatagatag atcggcttgg ctctcatggg aaggtcctaa aagtacgcag
     5221 tccaagcttg gcggcggtgg ccgcgagcca cagcagtacg gcaatcagcg catagcccgg
     5281 aaactgcagc tctcgcgagc ggtactcgcg cagctccact ttgccgggcg cgagctgatc
     5341 gatttcgtgc acggctcgca ggatcgtcgc ctcgtccgcc gcggggtaga agcggccgcc
     5401 cgtctgctcg acggcttcct tccacaactc gtccggcatc acggcgccga gtcccttgtt
     5461 gaaagcgacg cggatcatgt acaccgggat cccgtgcgcg cgagcgtcgg ccatgatgtg
     5521 tttgatgggc cggccctgca ggatggtctg gctgtcctgg ccgtcgctga agatcacgat
     5581 cagattgccg gccgcgccga gaaagtcgaa ggtttcgaac aactccgtac tcttctggat
     5641 ggcgcgcatg atgatcgtgc cctggtccgg gaagcgctcc cattcggccg gttcggcgat
     5701 cagcctcatg ctcagcagca cgttctcgta gtcggtggtg aacggggtca ccacgtaggc
     5761 ctcgttgccg aactccacca gcgcgatgag atcgcggaac gtgccttcca tgcgcagccg
     5821 catgaaatat tccgctgccg ccaccgtcgc aaagtaggta gggccaccct gcttgttgaa
     5881 gctgggggag tgaaaaggct ggttcatgct cgaggacgca tcgatcagca ccgcgatgcg
     5941 ccgcccggga aaggtcttgt cctcgcgcac cagcgttgtg aagggatcgg cgagcgccag
     6001 cagggcaaac cccagcccgg cgacgaacaa cacgaacggc gcgtggcgca tggccgcgcc
     6061 agccgatggc ctgactaccg gcagcaaggc cgccagcgcg atcgcgtggg gcttgcgccg
     6121 ctgttgctgg cgccgggcat gccacgcgat gagcagcgcc gctgccatcg acaatcccgt
     6181 cgcaacgaga atggccgcct ccatgcgtcc gaagcgcagc tcctccggtc gaagctgcag
     6241 ccactcccac aggacttccg caatcatggc tacgccgcac cggttgccac gcgctttccc
     6301 gccaggaatt gcagacgcga gggcagcaat gacgcgacga gcaactcgcg cgacgcgccc
     6361 agcgcatcct gtatcgccct gcccacctcg ggcatgccgc catcggcaat ctcgggccca
     6421 taacgaacgt ccgaacagac ggcgatcgcc accgccagtt cttcaagcga agcgtgcgcg
     6481 cgcagctggg ccgccgtgac ggtggccgag atggcgacgt atggcctgcg cagcgacagc
     6541 cggcgaagca accattcgcc attcccggca ggccgatcgc gagccgcaac ctgctggcgc
     6601 accggcaacc ctgcggccac ggcgccgagc acgcgcagca ccgccaatgc ctcgcccacg
     6661 cgcgcatcgg tccagccctg cttgccggct gccgagacct gcttgcgcaa tgcggctcgg
     6721 gcgcccagca cgagacgcca gtccgggatg cgaaacgccg gcttcacgcg gcgcgtgcgc
     6781 atcgcgcgcc aggcgctgcc caggaacagc agtagataga gagcgccggc cgccaggagt
     6841 ccacaactca ccaggaagac catggaggcc tggctgcgtc ggcgctcgat ggaaacgaac
     6901 gagtgcgccg tgacatcctc gatgtcgctc gcgcgtgcgg gcaccagcgc cagtatcttc
     6961 atcggcaacg gcgggagcac gtatgttcgc tcacggccca tctggacatt gccgccgcgc
     7021 gggccgaccg cgatgctgta ggcgatctcg agtggcggca gcggaacgtc cagcccgaag
     7081 aactcttcgc ccgtcagccg caacgtgtat tcgaactgcg tgtagcgcca cggcggcgtg
     7141 acgacgtctt cactgcgggt tcccgccacg acttcgaaag gggccagatc gacgacgtcc
     7201 ggttgcagct gatcccagtc cggcatcacc gtggccgcgt cggtttcggc ggcggcgcag
     7261 gtgagcgtga gccgcagggg ttcacccaca tgcaccgcca cgcggtcggt gcgccacccg
     7321 cattgcagcg gctgcacctc cgcctcggca tctgcaccga cactggcact ggcaccagcc
     7381 aacagcatca gcaatgtgag acagcgtcgc attcaacctc gcatcttttg caaccggcgg
     7441 ctggcgaaac aggcggagag cgtttcttcg agctcccagc ggcccgcgcc cacccgcacg
     7501 atatcgaggc cacgagaccg cgcgtgcccg accatcgcgg tctgccattc gtcgatacgc
     7561 tcgatcatgg ccgcggcggc cgcgcgcgaa accgtgcggg actcgccggt ttcgacgtca
     7621 tggatctcga gccatccggc gctgaccggc ggcaccgcga acaccggtcg tgcatccacc
     7681 atcaccacca gtacgtcgtg ttcgcccgtg agcacggcga agcgctcgag cagctcgtcg
     7741 atgccggtca gcaggcagtc cgacaccagc acgagcagcg atgagccgcg caaataagat
     7801 tcgagctgcg ggatcaactc gccactgccg gcctttcggt catcgctgca ctgcgcgtac
     7861 agcgacaggc aatgcatcac atgcgccttg cccgcgcgcg ggcgcaaggt tgccagcggc
     7921 cggcagagat cgtcgaacgc gacgatgccg aaacggtcct gactgaaagc cgccgacaga
     7981 cccaacaccg cgagactccg caggatggtg gtgcgcagtt gcgcgcccgc cgggccgcag
     8041 tgggtagata gcgaggcatc cgccgcgacc atcacggtgg cggcgctgtc ctgctcgagc
     8101 tcccgcgtga tcagcgggct gaagttgttg atggaggact gcgcccagtc gatcgacgac
     8161 atcccgtcgc ccggctccca ctcgcgcagc ccccgcacat cgaagccgtt gccggcgaat
     8221 acgctggcgt gatcccccga agcgtgttcg cgcatgcgtc gcaacaggaa tgtctcgatc
     8281 gccgatacgt cggcgggatt gatcagccag cctggctccc gcgcggcggg attcacggga
     8341 ccgccacctg gtggaccaca tcgtcgatca cggcttcgac ggtgatgccc gcacttgccg
     8401 cctgcggcgc cagccagacg cgatgaccca gcacgtaacg cgccagctcc tgcacatcct
     8461 cgggcaacac gaacgacctt tttcgctgca gcaccgccca gaccttgacc agacgccccc
     8521 agcagatcgt ggcgcgcggt gaggcgccga gttccacgta actctccacc agcgccgacg
     8581 gcgaacgctg agtccagccg gaggccgcgt cgagcggccg agtggcggcg accagccgct
     8641 ggatgtaagc ggcgatgcgc tcgtgcagaa acacttcacg gctgatcgcc gcgcgcagtt
     8701 cgagaatgcg gtccttggtc gcgagtggct tgaggctgac tttcttgaag tcgaagctga
     8761 ccagcttctg ttcctcctgc ggcggcagat agggcacgtt gacgatgatg gcgaagcgat
     8821 cggtggcggc ctcggagagt gcgaacgtgc cctggccgag ctcgatcgga ttcatggtcg
     8881 cgagcgcaaa gctgaattcc ggcaggtcgc aggtcgtctt gcccaccgtc accgtgcggt
     8941 cctgcagggc ttcgaggaag gcgctttgcg atttcaccgg aatgcgattg atctcatcga
     9001 gcaggatgat ttccgccgat gccagcggac cgaactccgt gacgaattcg ccggtcgcgg
     9061 ggttgatcat ctggaagccg accacttcgg ccggatgcag gtcggcgcgt ccctggatcc
     9121 tcgcgaactt cgcatcgctg atggcggcga gaatcacgcc gaagaacgtc ttgcccacgc
     9181 cgggaacgcc gcgcaacagc aggttgcccg cattcacctg gcgcttttgc cccttgtcgt
     9241 agctcgtggg gatctccgcc atgagcacga tgttggcgag cgccttttct ttctcgtagc
     9301 cgatgagcgc cttgccgccc tcggccagca cggcttcgtg aaaggcgatc gcctcggtca
     9361 ggtcggcatg catgtcattg cgccgtgtac ttctgcagcg agatgagcag ctgctcgtac
     9421 gcggcatcgg ccgcatcgac gtgcggcttc agcaggggac gctgcagcag ccattcctcg
     9481 gccagcacgg ttcgcgtttc ccacggctcc ggcccggtct gcggttcgat gcgctcgacc
     9541 atgatccgct gcaattgctg ccgcagctca ctcacgatcg tgggcagcgc ggcgcgattc
     9601 cacgtcggct cgtcgtccac caccacgtag ataggcgtgc tgtgcgcaat cgtcatgttg
     9661 tccggatgct ggcggccgcc ataggagcgc acggctatcc acatgctgcg gtcggccacg
     9721 agctgatggc ggatgcccgc gttgtcggcg cctttggccg gcgcgctcgc ggcgacttca
     9781 ccgaggacta tcaactccag ccggtcgagt gcgtcgacat cgggattgag ctgcgtcgcg
     9841 gcggtgatgt cgagcaccgt gccgcgcttc accttgagtt cctggcccat gccgcgcccg
     9901 ttgatgctga actcgagaaa cggcccgttc gtgacgtagg tgtggccggc gcgaaacgca
     9961 gcgaaccacg catcgagatt gagaggtccg tcgagcttga cgaaattgcg caccacaccg
    10021 gggaagtcgc tatacggcca gtccgagccg gccgcggggt tgatgcggta acccaggttc
    10081 agcagtcgat accaaccttc gtggatcaac ctgccccgct gcagcacttc gatgaagttc
    10141 accaggcccg tgggcgcgag aatcgcaaga ccacgattga actgcccttg cggcgcgatc
    10201 tgcccggcgc cggtactgcc cgcctggttc catcccatgt gcgcgaagcc ggagatgccc
    10261 ccctggcgtg ccgattcctg aaacaccttg tgataaagga agtagctgtc cgacggcgcg
    10321 cgaatcggac tctggatgtt gtgatgaatg gtgtgcccga agtgaccggt gcgcgggtct
    10381 tcctgaccgg agacgatgaa gtgtccgtcg cgctcgaagc ggctggcctt gccccaggct
    10441 tgcggctgat ggaagtggag cttcgcgatg ttgcccatct cgagcaggtt gcctacgtac
    10501 acatcttccg ccgcgacgaa gccccagatc tgcggatcgg caacctggtc gcgcgtcacg
    10561 tgcacatgcg cgtcgccgga ataccagccc ttcgccggca ggttcgcgta acgctgcaaa
    10621 gaaacgtcga ggcgcgaggt cgcgccgctc ttgatctcga agctgctccg atgtgcgcga
    10681 aactccggtc cgcgggtcac gacgagttcg tactggccgg cgggaagtct ggcgcggtac
    10741 tcgccgtcga catagaaggc ctgccggtgg tcggacggcc agaaggttcg ttcgttgacg
    10801 gccagcatgc gcaggtcatc ggcgaaccgc tgcagcttca gcgaatcgtc cgaggccagc
    10861 ggcgcacggc cggtggcgtc atagattccg acccgggccg gcacgcgccg gccggtggga
    10921 ccgtccttga tggtcaaaag cgcttcgccg aaagccgcgg gcagcggtgt ggagttgctg
    10981 cgctcgagcg tgatgtcgca gatcgccacg ccggagcgag tgccgatcgc gatgtctgcg
    11041 ccctgggtgg cctggccggc gaatcgcgcg cgctccagga ctaacttctg cttctgaaaa
    11101 cccgccgcgg gctggggcac gatctccgcg ctcttgccct gcccggtgcc gccattgcgg
    11161 tcccaggcca cgatgaaagg tgatgagtag tcgctggcgt aggtcagcgt cagcgtcacg
    11221 gtctcgtcga tgtcgaacgc gaatgcattg tccacgtcga acagcagcag atcgccgacc
    11281 acgcagccct ggcccgcgat gacccgcgcc tgcaccgggt tgctggtggt cgcaatcccg
    11341 tcgagatgcg cgcgattcga accgaaggtg cgcgccacat tgggcttctt ccagttgagg
    11401 atctcgaggt atcgcccttc ggtgccgtgc ccgaaactcg ccacggacag caaggctgtc
    11461 aggcatgcga acgcgactct tcccatggct ttcttcccct cagtcggctt tcgcgagctt
    11521 ttgatccccg gcgggcttgg gggccggggc attgcccagg tacttcagcc actcacccgc
    11581 ctgcttgcgc gattcgtcga acttcatggc ttgttgcagc gccgcctggg tgcccggcac
    11641 gttgctgagg ttgtagttag ccaccgcaat acgcatccat gcttcgcccg gccgcttcaa
    11701 cccgcccttc tggacggcgc gctcgagcgc ctgcctggag tcactccact gttcgttgtc
    11761 gccgtacatg gcgccgagct tgaagtagta ctcgccgcga tccgccatcg ccgccagctt
    11821 cttgagcgcg gcctcggc
//
LOCUS       DATDEU010000043         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1466770, whole genome shotgun sequence.
ACCESSION   DATDEU010000043 DATDEU010000000
VERSION     DATDEU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466770"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            534..659
                     /locus_tag="VIV63_00915"
     CDS             534..659
                     /locus_tag="VIV63_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123183.1"
                     /translation="MPFESLLEREAVVVFDFDPSVVEIVAQPFALLWPLLQDGAA"
     gene            692..1513
                     /locus_tag="VIV63_00920"
     CDS             692..1513
                     /locus_tag="VIV63_00920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6123184.1"
                     /translation="MSAVQGHYTRDDIAQRVLAAARAAAGPGVPLTPDVLAPIDHFHG
                     GGLASTRELMVLLEPSAGETVLDIGCGIGGPARWIASTFGCQVTGIDLTAAFCRAAEM
                     LNAETGLGDKVKIFEASALALPFEPKSFDRAYSQNVVMNIADKKGFYREAFRVLRPGG
                     VLAMANLCAGPGGEPYYPAPWATTAATSFLSTPEETRRDLEQTGFEVVRFEDTSARTA
                     AARAAQRLRLESEGLPALGFHVFMGAHMLEAMINTARSAEEGRGTSVEILVRKPG"
     gene            1592..2014
                     /locus_tag="VIV63_00925"
     CDS             1592..2014
                     /locus_tag="VIV63_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEY6123185.1"
                     /translation="MTEASSSSPHPAPHPDGLSMLEKRRIEAEILKEVYLTLKESHGE
                     EMAKKTIAESVRRSAIEQAQAFAAAAPGGPSLKAFQDVMPLWTKGGALEIEVKEQSDK
                     SFAFNVVRCRYAETYKAMGLGEIGHLLSCNRDGAFCEG"
BASE COUNT          360 a          680 c          749 g          360 t
ORIGIN      
        1 agcacacctt ctacatccag gatgggcgcg cgggaggcgc ggagtacgaa gccgtggacc
       61 ttgcagacct ctgtatcgcg gagtggcgat cattcttgag cgcgcaccag ctcgaccatg
      121 gcctgaatgg gttctcctga ccggcgagtt ctcaccaccg aataactcgt cctgctcaac
      181 tcgcagcgcg accatcgtcg agtccttccg cgatcctcat gggttgcctc tgttcgtcac
      241 cgacagtcgc tgccacactc tggtgagggg ctgagggttg cctgtgcccg cgcgggaggt
      301 gacgtgcgat catgcttgcg cactcccccg ccgtgtcacc gatcccagcg aacctgtctg
      361 accgggtgcg tattgcatgg atcgatcacg atggtcgatt agtcctccgt gacctgactg
      421 agagcctcga tgttggtgtg ctgttcgaag gacgggcggc gagaaggttt ccggcgtggt
      481 cgggaaagcg aagtttcagc ggttggtatt ggtgccggac gactggttcg atggtgccgt
      541 tcgagagtct gctcgagcgc gaggcagtcg tggtcttcga cttcgatccg tcggtggtcg
      601 agatcgtggc gcagccgttc gctctcctgt ggcccctgct acaagatggg gctgcgtgat
      661 acgaaagagc atggcggaga gcgagggaca ggtgagcgcc gtccagggcc actacacgcg
      721 ggacgacatc gcccagcgcg tgctcgccgc ggcgcgcgcg gcggccggtc ccggggtgcc
      781 gctgacacct gacgtgctgg cgccgatcga tcacttccat ggcggcggcc tcgcttcgac
      841 gcgcgagctc atggtcctgc tcgagcccag cgccggcgag acggtgctcg acatcggctg
      901 cggcatcggc gggccggcgc gctggatcgc cagcaccttc ggctgccagg tgacgggcat
      961 cgatctcacg gcggcgttct gccgggccgc ggagatgctg aacgccgaga ccggcctcgg
     1021 cgacaaggtg aagatcttcg aggcctctgc gctggcgctg ccgttcgagc ccaaaagctt
     1081 cgatcgcgcc tattcgcaga acgtcgtgat gaacatcgcc gacaagaagg gcttctatcg
     1141 cgaggccttt cgcgtgctgc ggcccggggg cgtgctggcc atggcaaacc tctgcgccgg
     1201 gccgggcggc gagccctact atccggcgcc gtgggcgacc acggcggcga ccagcttcct
     1261 gtcgacgccg gaggagacgc ggcgcgatct cgagcagacg gggttcgagg tcgtgcggtt
     1321 cgaggacacg tcggcccgca cggccgccgc gcgagccgcg cagcggctgc ggctggaaag
     1381 cgaaggcctg ccggccctgg gcttccacgt gttcatgggc gcacacatgc tcgaggcgat
     1441 gatcaacacg gcccgcagcg ccgaggaagg ccgcgggacg agcgtcgaga tcctggtgcg
     1501 caagccgggg tgaaacggtt tgtgtcgcgc ggcgcgccga tgcaggctgc acgagtccgt
     1561 ccgccggcta ggttgcccca gcgaggttgc catgaccgaa gccagttcgt cctcccccca
     1621 tcccgccccg catcctgatg gcctctccat gctggagaag cggcggatcg aagccgaaat
     1681 attgaaagag gtctatctca ccctgaagga gagccacggc gaagagatgg cgaagaagac
     1741 catcgccgaa tcggtgcggc ggtccgccat cgagcaggcc caggccttcg ccgcggcggc
     1801 gcccggcggt ccctcgctca aggcgttcca ggacgtgatg ccgctgtgga ccaagggcgg
     1861 ggcgctcgag atcgaggtca aggagcagag cgataaaagc ttcgccttca acgtcgtgcg
     1921 ctgccgctac gccgagacct acaaggcgat gggcctgggc gagatcggcc acctgctgtc
     1981 gtgcaaccgc gacggcgcgt tctgcgaagg ctaacgaccc caagctcaag ctcgagcgca
     2041 cgcagaccat catgcagggc gcgagccact gcgatttcaa gtatcgctac gagaggtgac
     2101 ggcgcgggac gcgccgtcag cgttttcggg atgctgaggc aaggccgga
//
LOCUS       DATDEU010000044         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1758419, whole genome shotgun sequence.
ACCESSION   DATDEU010000044 DATDEU010000000
VERSION     DATDEU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758419"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /gene="rplM"
                     /locus_tag="VIV63_00930"
     CDS             <1..252
                     /gene="rplM"
                     /locus_tag="VIV63_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY6123186.1"
                     /translation="EKIKVTGNKLEDKKYYHHTGAIGGIKEIALGKLLKEHPERAIEF
                     AVKGMLPKGPLGRKMYKKLHVFAGDKHPHAAQQPKQLEI"
     gene            264..662
                     /gene="rpsI"
                     /locus_tag="VIV63_00935"
     CDS             264..662
                     /gene="rpsI"
                     /locus_tag="VIV63_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY6123187.1"
                     /translation="MPATAKPNYGTGRRKTAAARVYMKAGTGKITVNDRPLDEFFGRE
                     TGRMIVRQPLETVQLANKFDFTVSVSGGGITGQAGAIRHGITRALIEYDEELRKPLRI
                     AGFVTRDAREVERKKVGRRKARRGPQYSKR"
     gene            696..769
                     /locus_tag="VIV63_00940"
     tRNA            696..769
                     /locus_tag="VIV63_00940"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:728..730,aa:Gln,seq:ctg)
     gene            836..>2584
                     /locus_tag="VIV63_00945"
     CDS             836..>2584
                     /locus_tag="VIV63_00945"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY6123188.1"
                     /translation="MKRLALLATMILATAVHAAAGDARNPVAQLNDMYAEFWEENLKL
                     NPMSATFAGDPRYNAELPNFLSPEFFEKTRAFEQKYLDRARAIGTDGLAGQDRLSYDI
                     FTLNRESALDDLKFPERLLPIDQFQNVANYFAQLGSGTSAQPFVTVKDYDDWLKRAAV
                     MPAILDQAITNMREGVKAGIVQPRVLMEKVLPQLDANIVTDVEKSIYWGPVTQMPKDF
                     SAADRDRLTSAFKVLIGTQLTPAYQRLRTYIADEYLPKTRDTFGMGALPNGAAWYAHK
                     VHDSTTRTMSPAKVHQIGLDEVARIQDGMRSVAKELGYTKPTKTLPELKAFFEWMKSR
                     DDMYFKSREELLSAYQAFDVNVAPILPNYFNLRPKAAYEVRLVEPFREASASSGQYQG
                     PSLDGKRAGVFFVNAYDLKARPRWALASLSLHEAAPGHHFQISLQRELGDLPMFRRFS
                     RDTAYIEGWGLYAEWLGYEMGTYKDPVARFGALDAELWRAIRLVTDSGIHYKGWTRQQ
                     TLDYMYANSPAEQTRAVSEAERFAAIPGQALAYKVGQLKIVELRHRAEKALGKKFDVK
                     AFHDEVLKDGAVPLDVL"
BASE COUNT          538 a          851 c          775 g          420 t
ORIGIN      
        1 gagaagatca aagtcacggg caacaagctc gaagacaaga agtactacca ccacaccggc
       61 gccatcggtg gcatcaagga aatcgctctc ggcaagctgc tcaaggagca tcccgagcgc
      121 gccatcgaat tcgcggtgaa gggcatgctc cccaagggcc cgctcggccg caagatgtac
      181 aagaagctgc atgtgttcgc gggcgacaaa catccgcacg ccgcacagca gccgaagcag
      241 ctcgaaattt aaggaactga agaatgcccg ccactgcaaa gccgaactac ggcaccggcc
      301 gccgcaagac cgccgcggct cgcgtctaca tgaaagccgg aaccggcaag atcacggtca
      361 acgaccgtcc tctcgacgag ttcttcggcc gtgagaccgg ccgcatgatc gtgcgccagc
      421 cgctcgaaac cgtgcagctc gccaacaagt tcgatttcac ggtgtcggtc tccggcggtg
      481 gcatcaccgg ccaggccggc gccattcgcc acggcatcac gcgcgcgctc atcgaatacg
      541 acgaagagct gcgcaagcct ttgcgtatcg ccggcttcgt cacgcgcgac gcgcgtgaag
      601 tcgaacgcaa gaaggtcggc cgccgcaagg cgcgtcgtgg accgcagtac tccaagcgct
      661 aatcacgcta gtattgttcg cagacggcca gcttctggga gatcgtctag cggtaggaca
      721 acggactctg actccgttta cctaggttcg aatcctagtc tcccaaccaa tatggaaccc
      781 gcccattctc tgaaaagaat gggcgggttt cttttttcag acggagtagt tagtgatgaa
      841 acgcctcgcg ttgctcgcaa cgatgatcct cgccacggca gtgcacgccg ccgccgggga
      901 cgccaggaat ccggtcgcgc agctgaacga catgtacgcc gagttctggg aagagaatct
      961 caagctcaac ccgatgtccg cgacgtttgc cggtgatccg cgctacaacg ccgagctgcc
     1021 gaatttcctg tcgccggaat tcttcgaaaa gacccgcgca ttcgagcaga agtacctcga
     1081 ccgcgcgcgc gccatcggca ccgacggcct cgcgggccag gaccgcctgt catacgacat
     1141 cttcacgctc aatcgcgaat cagcgctcga tgacctgaag ttcccggaac ggctgctgcc
     1201 catcgaccag ttccagaacg tcgccaacta cttcgcgcag ctcggctccg gcacgagcgc
     1261 gcagccgttc gtgacggtca aggactacga cgactggctc aagcgcgctg cggtgatgcc
     1321 ggcgatcctc gaccaggcca tcacgaacat gcgcgagggc gtgaaggccg gcatcgtgca
     1381 gccgcgcgtg ctcatggaga aggtgctgcc gcagctcgac gccaacatcg tcactgacgt
     1441 cgagaagagc atctactggg gtccggtcac gcagatgccg aaggattttt cggcagccga
     1501 ccgcgaccgc ctgacctcgg ccttcaaggt gctgatcggc acgcagctca cgccggctta
     1561 ccagcgcctg cgcacgtaca tcgccgacga atacctgccg aagacccgcg acacgttcgg
     1621 catgggcgcg ttgcccaatg gcgccgcctg gtacgcgcac aaggttcacg acagcacgac
     1681 gcgcacgatg agcccggcga aggtccacca gatcggtctc gacgaagtcg cacgcatcca
     1741 ggacggcatg cggtcggtcg cgaaggaact gggttatacg aagccgacca agacgctgcc
     1801 cgaactcaag gcgttcttcg agtggatgaa gtcgcgcgac gacatgtact tcaagtcgcg
     1861 cgaagaactc ctgtccgctt accaggcgtt cgacgtcaac gtggcgccga tcctgcccaa
     1921 ctacttcaac ctgcggccca aggccgcgta cgaagtgcgc ctcgtcgaac cgttccgtga
     1981 agcgtctgcg tcgtcgggcc agtaccaggg cccgtcgctc gacggcaaac gcgctggtgt
     2041 gttcttcgtg aatgcctacg atctcaaggc ccgtccgcgc tgggcgctgg cgtcgctgtc
     2101 gctgcatgaa gccgccccgg ggcaccactt ccagatctcg ttgcagcgcg aactcgggga
     2161 cctgccgatg ttccgccgct tctcgcgcga caccgcctac atcgaaggat ggggcctgta
     2221 cgccgagtgg ctcggctacg agatgggtac ctacaaagac ccggtcgcgc gcttcggtgc
     2281 gctcgacgcc gagctgtggc gcgccatccg gctcgtgacc gacagcggca tccactacaa
     2341 gggctggacg cgtcagcaga cgctcgacta catgtacgcg aattcgccgg cagagcagac
     2401 gcgcgccgtg tccgaagcgg aacgcttcgc ggccattccc ggccaggcgc tggcgtacaa
     2461 ggtcggccag ctcaagatcg tggaactgcg tcatcgcgcc gagaaagcgc tcggcaagaa
     2521 gttcgacgtg aaagctttcc acgacgaagt gctgaaagac ggcgcggtgc cactcgacgt
     2581 gctc
//
LOCUS       DATDEU010000045         3944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_737356, whole genome shotgun sequence.
ACCESSION   DATDEU010000045 DATDEU010000000
VERSION     DATDEU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737356"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VIV63_00950"
     CDS             complement(<1..300)
                     /locus_tag="VIV63_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123189.1"
                     /translation="MSPYSAAPALSVASPTGVDAHLVLAGPGARSLAFLLDWLIRSAL
                     GAIYLLLASWLILGNFRLDIGPDEETLWYLAGALPATAIYFLYHLILEPLMAGRTP"
     gene            complement(345..1439)
                     /gene="prfA"
                     /locus_tag="VIV63_00955"
     CDS             complement(345..1439)
                     /gene="prfA"
                     /locus_tag="VIV63_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY6123190.1"
                     /translation="MKDTIRLRLEKMVDRYEEIGRSLADPAVIGRQREFRELSQEYAR
                     LTPVAEKFGAFQALERELTSARDMQSDKDPGMREMGQEEVTRLMPEIERAEDALKMLL
                     IPRDPRDDKNIYLEVRAGTGGDEAAIFAGDLYRMYARYAETHGFTVEILSESQGEHGG
                     FREVISRIAGPGAFARFKFESGVHRVQRVPATEAQGRIHTSACTVAILPEPDEVEATD
                     LNPAELRIDTFRASGAGGQHVNKTDSAIRITHIPSGLVVECQDERSQHKNRSRAMALM
                     KAKLMAAEQEKRDSEVARNRKLQVGTGDRSERIRTYNFPQGRVTDHRINLTLYKLGDI
                     MTGKIDELLDSLQHEHQAEQLAEEEQRGAA"
     gene            1483..3246
                     /locus_tag="VIV63_00960"
     CDS             1483..3246
                     /locus_tag="VIV63_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6123191.1"
                     /translation="MRRNTSSRARSLGLAMVIGVLLPVGAFALSTNNSDTADLVLADM
                     ALSRGDCRGGTDRYVKAAFATNDPKISQRANEVASECQQIAASAKVARRWQKLEPDSA
                     SASAAVALAAVQLYQPAEASAAILRTGALGGDETLIKLMAVVSDAGGSAIALKTLRPT
                     LESPEVSDRLLAAGIDLALENFDFAIAHRLADRMLANEPASGTARAQLARVLTAEGNA
                     VAAIAVAQEAAALDPDTERFAYADTLMRLDRLDEARQELENMRSDASVRDEADLRLGK
                     LAYQMGDMSEAGRRFGSLVGSQATALEAFFYLSAIAEREGRTDLALEGYTKLVEAGAG
                     LIVRGRAARLLLKKDERSAAFRMLDAHADKERAETLDVEFAKAALLDDVGRSTEAIAL
                     LQLALERFPDHPGVRYQIALIQDKAGMPRESVRSFESLLKERPEDASLLNALGYSLAD
                     RNEKLPRAETLIRKALEASPDNPAFLDSLGWVLFRRGDIPAALPYLERAYRIFPDAEI
                     ASHWGELLWASGKQSEARALWARSLARSPDSKPLRSTIERLTGTKMDDTAPKSDAPAP
                     GAEGAPAPLQQPAPTSTSTSS"
     gene            3249..3860
                     /gene="lolB"
                     /locus_tag="VIV63_00965"
     CDS             3249..3860
                     /gene="lolB"
                     /locus_tag="VIV63_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEY6123192.1"
                     /translation="MRAAVAASFWSGMGILLLLAGCRTAPPTGVIGPGADAPWSEQRA
                     ALEKLDRYGLNGRVAVAAQGQGFSAGLRYQQEPRRSNLALDGPLGIGGMRVELEGTGI
                     SIETSRGEKLDGEAARGELERRLGFALPLAELRWWLLGIPAPGESSINQDAGSGEIHD
                     FTQNGWRVSINSRAAGLGFALPQRLTAERDGARLKLLVDNWQP"
BASE COUNT          646 a         1293 c         1333 g          672 t
ORIGIN      
        1 cggcgtgcgg cccgccatca gcggctcgag gatcaggtga tagagaaagt agatagcagt
       61 ggccggcagg gcgccggcga ggtaccacag cgtttcttcg tcgggaccta tgtccagccg
      121 gaagttgccg aggatgagcc acgaggcgag cagcaggtag atagccccga gcgcggagcg
      181 gatgagccag tcgagcagga acgcgagcga ccgggcgccc ggacccgcca gcaccaggtg
      241 agcatcgacg ccggtggggc tggcgactga cagggcggga gcggccgaat atggggacac
      301 tcctgatttg ctagcaaaag gggacgcgcc tcctgccgcc gaattcacgc agcgccccgc
      361 tgttcttctt ccgcgagctg ctccgcctgg tgctcgtgct gcagcgagtc cagcaactcg
      421 tcgatcttgc ccgtcatgat gtcgccgagc ttgtacagcg tgaggttgat gcggtgatcg
      481 gtgacgcggc cctgcggaaa gttgtacgtg cggatgcgct cggagcgatc gccggtaccc
      541 acctgcagct tgcggttgcg cgcgacttcg ctgtcgcgct tttcctgttc cgcggccatg
      601 agcttggcct tcatgagcgc catggcgcgc gaacggttct tgtgctgcga gcgttcgtcc
      661 tggcactcga ccacgaggcc ggaaggaatg tgcgtgatgc ggatggccga gtcggtcttg
      721 ttgacgtgct ggccgccggc gcccgaggcg cggaacgtgt cgatgcgcaa ctcggcggga
      781 ttgaggtcgg tggcctccac ttcatccggc tcgggcagga tcgcgacggt gcaagccgag
      841 gtatggatgc gaccctgcgc ttcggtggcc ggcacacgtt gcacgcggtg cacaccggat
      901 tcgaacttga agcgcgcgaa cgcgcccggg ccggcgatgc ggctgatgac ttcgcggaat
      961 ccgccgtgct cgccctggct ctcggaaagg atctccaccg tgaagccatg agtctcggca
     1021 taacgcgcgt acattcgata gaggtcgccg gcgaagatgg cggcctcgtc gccgccggtg
     1081 ccggcgcgga cttcgaggta gatgttcttg tcgtcgcgcg gatcgcgtgg aatgagcagc
     1141 atcttgagcg cgtcttccgc gcgctcgatt tcgggcatga ggcgcgtgac ttcctcctgg
     1201 cccatttcgc gcatgcccgg gtctttgtcg ctctgcatgt cgcgtgccga cgtcagttcg
     1261 cgttcgagcg cctggaacgc gccgaacttc tcggccacgg gagtcaggcg ggcatattcc
     1321 tgcgataact cgcggaattc gcgttgtcgg ccaatgacgg cagggtccgc cagcgagcgt
     1381 cctatttcct catagcggtc gaccattttt tcgagccgca gtctaatggt gtctttcacg
     1441 cgaaccattg taaggggcga aggtccagtt atagtgcctt ccatgcgacg aaacacatcc
     1501 tcacgggccc gatctctggg cctggcaatg gtcatcggtg tgctcttgcc ggtgggcgct
     1561 ttcgcgttgt ccaccaacaa ttccgacacc gccgatctcg tgctggccga catggcgctg
     1621 tcgcgcggcg actgccgggg cggcaccgac cgctacgtga aggcggcctt cgccaccaac
     1681 gatcccaaga tttcgcagcg cgccaatgaa gtcgcgtccg aatgtcagca gatcgcagcg
     1741 agcgcgaaag tcgcgcggcg ctggcagaag ctcgagccag acagcgccag cgcgtccgcc
     1801 gcggtggcgc tcgcggccgt ccagctctat caacccgccg aagcatccgc ggccatcctg
     1861 cgcacgggcg ccctgggcgg cgacgaaacg ctcatcaagc tcatggccgt cgtcagcgat
     1921 gcgggcggca gcgccatcgc actgaagacg ctgcgtccca cgctcgaatc gcccgaggtt
     1981 tccgaccggc tgctggccgc gggcatcgac ctcgcgctcg agaatttcga tttcgccatc
     2041 gcgcaccggc tggctgatcg catgctcgcc aacgagccgg cgtcgggcac cgcccgtgcg
     2101 caactcgcgc gcgtgctcac ggccgaaggc aacgcggtgg cggctattgc cgtcgcgcag
     2161 gaagccgcgg cgctcgatcc ggacaccgaa cgtttcgctt acgccgacac gctgatgcgc
     2221 ctggatcgcc tcgacgaagc gcggcaggaa ctcgagaaca tgcgcagcga tgcgtcggtt
     2281 cgcgacgaag ccgatcttcg cctcgggaag cttgcctacc agatgggcga catgagcgaa
     2341 gccgggcggc gcttcggtag tttggtcggc tcgcaggcga ccgcgctcga agccttcttc
     2401 tatctgtcgg ccattgccga acgcgaaggc cggaccgatc tcgcgctcga gggttacacc
     2461 aaactcgtcg aggctggtgc cgggctcatc gtgcgcggcc gcgcggcgcg ccttctgctc
     2521 aagaaggacg aacgctccgc ggcgttccgc atgctcgatg cgcacgccga caaggaacgc
     2581 gccgagacgc tcgatgtgga attcgccaag gccgcgctgc tcgacgatgt cgggcgctcg
     2641 accgaagcca tcgcgctact gcagctcgcg ctcgagcggt tccccgacca tcccggtgtg
     2701 cgttaccaga tcgcgctgat ccaggacaaa gcgggcatgc cgcgtgaatc cgtgcgcagc
     2761 ttcgaaagcc tgctcaagga acggcccgag gacgccagcc tgctgaacgc gctgggctac
     2821 tcgctggccg accgcaacga aaaactgccg cgtgccgaaa cgctcattcg caaggcgctc
     2881 gaagcctcgc cggacaatcc ggcgtttctc gacagtctcg gctgggtgct gttccgccgc
     2941 ggcgatatcc cggccgcgct gccttatctg gagcgcgcct accgcatctt ccccgacgcc
     3001 gaaatcgcct cgcactgggg cgaactgctg tgggcgagcg gcaaacagtc cgaggcgcgc
     3061 gcgctgtggg cgcgatcgct ggcgcgttcg cccgattcca agccgctgcg ctccaccatc
     3121 gaacggctga ccggcacgaa gatggacgac accgcgccca agtccgacgc tcctgcgccc
     3181 ggtgcggagg gcgcgccggc tcccctgcag cagcccgcgc ccacctcgac ttccacgagt
     3241 agttagcggt gcgtgccgcg gtcgcggcct ctttctggtc ggggatgggc atcctgctgt
     3301 tgttagccgg ctgccgaacg gcgccgccga cgggcgtcat cggcccgggc gccgatgcgc
     3361 cgtggtccga acagcgcgcc gctctcgaga aactcgaccg ttatggcctg aacggccgcg
     3421 ttgcggtggc cgcacaaggg caggggtttt ccgcgggcct tcgttaccag caggagccgc
     3481 gccgttcgaa tctcgcgctc gatgggccgc tgggaatcgg cggaatgcgc gtcgaactcg
     3541 aaggcaccgg aatctcgatc gagaccagcc gcggtgagaa gctcgatggc gaagccgcgc
     3601 gtggcgagct cgagcgaaga ctgggtttcg cgttgccgct ggcggaattg cgctggtggt
     3661 tgctcggaat tccggcgccc ggcgagtcga gcatcaacca ggatgcaggc agcggcgaga
     3721 tccatgactt cacgcagaac ggctggcgcg tcagcatcaa ctcgcgtgcg gccggactcg
     3781 ggttcgcgct gccgcagcgg ctgacggccg agcgcgacgg ggcgcgcctc aagctcctgg
     3841 tagacaactg gcagccgtga gcaagtcgaa gatggtggtc tggtccgcgc cagccaaact
     3901 caacctgatg ctgcacatcg tcggccgcga tcagggcggg tacc
//
LOCUS       DATDEU010000046         6006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1467136, whole genome shotgun sequence.
ACCESSION   DATDEU010000046 DATDEU010000000
VERSION     DATDEU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467136"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /gene="aspS"
                     /locus_tag="VIV63_00970"
     CDS             complement(<1..1386)
                     /gene="aspS"
                     /locus_tag="VIV63_00970"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY6123193.1"
                     /translation="MRSHYCGQVNEKLVGQEVTVAGWVHRRRDHGGVIFVDLRDREGL
                     LQVVFDPDNQAQFNEAEKLRNEFVVKIRGLVRERPAGTANANLGSGKVEMLARELVTL
                     NRAEPLPFQLDEHVSEEVRLKYRYIDLRREEMSKRLRLRHKITRAMRAYLDDAGFVDI
                     ETPVLTKATPEGARDYLVPSRTHAGKFFALPQSPQIFKQLLMVAGFDRYYQIVKCFRD
                     EDLRADRQPEFTQLDIETSFLSQEQIMELMEKLTRHFFKDGIGIDLADPFPRMGFAEA
                     MRRFGSDKPDLRNPLELVDVADLVASCDFKVFAGPAKDPKGRVAALRVPGGGAMPRSQ
                     IDEYTAYVARYGAKGLAYIKVNETAKGKEGLQSPIVKFLSDDSVKGILERTGAKDGDL
                     IFFGADSAKVVSDAIGALRLKVGVDQKLTTEGWKPLWVVDFPMFDWDPDEKRWVAMHH
                     PFTSPVNDDVAA"
     gene            1523..2281
                     /locus_tag="VIV63_00975"
     CDS             1523..2281
                     /locus_tag="VIV63_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123194.1"
                     /translation="MLAFLFLASGCSKDDVLQKFASKEEQAVASGYIEKLRQGQISDI
                     EAQLDVSLRKPGVGDTLTKMSAMVPKGKPDSVTLVGARTQYWNGAKSVNLTYQYQFGE
                     QWFLINCASHGEGAALRILGLTVTPMAQSLDEQNKFTLGGKNWVQYTVLAAAITFALL
                     SIYALVLCARDKSLRRKWAWVLFVMFGVCKFAVNWTTGEWGFTPLAIQLFSSGAAADL
                     YGPWIISTSLPLGAVWYLLRRRRNQIPAPLSNPI"
     gene            2304..3008
                     /locus_tag="VIV63_00980"
     CDS             2304..3008
                     /locus_tag="VIV63_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY6123195.1"
                     /translation="MTRSMTALFASVAFFTMGAAAHAQSANWVAGKHYSVIPTQRTNV
                     PAGKVEVMEVFSYGCPACNGFLPVMKKIKASLPANAQLVYLPASWNKAEAWPMFQRAY
                     LTAQSLGVAEKAHDEMFKAIWATGELGVTEPSGRLKKNLPTIENAAAFYQRVAGVKSA
                     DFVNASKSFTVDLKMRQADSQIIAMQVSGTPTLVVNGKYRLNNDALTTEQMVDLVKFL
                     VAKESAPAAAAPAKKG"
     gene            complement(3005..4006)
                     /locus_tag="VIV63_00985"
     CDS             complement(3005..4006)
                     /locus_tag="VIV63_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6123196.1"
                     /translation="MHKQLLCLPLFALLMLPAQAQEPGTNPLVGAHTLRMPWNAGAAD
                     CSASKSNEVIEAHHYSSDTHVIRQNPCVDYEANLIYLLVGKERSLLVDTGAVDGPNAG
                     RTAFVVENMLQTDGADQRPLLVVHTHGHLDHRAGDAEFAKLSGVEIAPIESEPLRKFL
                     GLADWPNGLGQIDLGGRIVDLIPAPGHHPDHIVFYDRNTKLLLTGDFLLPGRLLVDDI
                     DAYRASAQRVADFVKTHPVDYVLGAHIELDVGGEAYPSGSTYHPNERALPLSADDVLA
                     LPAALADFNGFYSGHANFIVVNPIRNLLALAAAGLAALTLLVWLGRRLWKRRRAAVA"
     gene            complement(4079..5449)
                     /locus_tag="VIV63_00990"
     CDS             complement(4079..5449)
                     /locus_tag="VIV63_00990"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY6123197.1"
                     /translation="MSAHTQIADKAELAAFLAAHPDIHAVQIMITDPSGVLRGKAVRR
                     EELERIFDSGRQVAGSILGLDITGKDVDDTGLVWDTGDADMTARPIANSLKPAPRLAA
                     PTGQLMLTMFDAQGLPAAADPRHALVRVVERFARTGLTPVVACELEFYLLKEEAGGRL
                     VPAGGGRASERQKIDAYSLQRLDDLAPMFNDVYTAAVAQGLPAETLMSEYSPGQFEIT
                     LHHRADALRAVDEAVMFKRVLRGVAQKHGLIACFMAKPFTERAGSGLHVHVSMNDTNG
                     ANLFASDDPAGTPELRQAIGGMKDSMAESMAVFAPNANSYRRFVSESYAPIAPIWGIN
                     NRSVSLRVPAGPAISRHVEHRICGADANLYLAVATVLGAAAHGMRERLDPGPAVDGNG
                     YAQAGERTLPGSWPEALKLAAKSEFLEETLGREFLKIFLAIKNQECARFSGEVSELDY
                     AWYLRS"
     gene            complement(5446..>6006)
                     /locus_tag="VIV63_00995"
     CDS             complement(5446..>6006)
                     /locus_tag="VIV63_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6123198.1"
                     /translation="GYARAQNLEPDIWVCGKAIAGGMPCAVFGFTTEVEAGMRRVQED
                     RGGGHSGMGTTLSANALAMACLEASLDELMTPANYQAMHDCASYLSEGLGRMFAAQTL
                     SWHVARVGARLEFGFGKPARNGVDSERSAWPELEHAIHLYLLNRGVLLTPFHNMMLCS
                     PVTTTSHADTLLKHLHACLREVGSPS"
BASE COUNT         1089 a         1926 c         1913 g         1078 t
ORIGIN      
        1 cgccgccacg tcgtcgttca ccggcgaagt aaacgggtga tgcatggcca cccagcgttt
       61 ctcgtccgga tcccagtcga acatcgggaa gtcgacgacc cacagcggct tccagccttc
      121 ggtggtcagc ttctggtcca cacccacctt caggcgcagc gcgccgatgg cgtcgctaac
      181 taccttggcg ctgtccgcgc cgaagaatat gaggtcgcca tctttcgctc cggtgcgctc
      241 gagaatgccc ttcaccgaat cgtcggacag gaacttgacg atgggcgact gcagaccttc
      301 cttgcccttc gccgtctcgt tcaccttgat gtacgcgaga cccttggcgc cgtaacgcgc
      361 gacatacgcg gtgtattcgt cgatctgcga acgcggcatg gctccgccgc ccggcacgcg
      421 cagcgcggcg acacggccct tcggatcttt cgcgggaccc gcgaacacct tgaaatcgca
      481 gctcgcgacg agatcggcca cgtcgacgag ctccagcgga ttgcgcaggt cgggcttgtc
      541 ggaaccgaag cggcgcatgg cttccgcgaa acccatgcgc gggaacggat cggcgagatc
      601 gatgccgatg ccgtccttga agaagtgacg cgtcagcttc tccatgagct ccatgatctg
      661 ttcctgcgac aggaacgagg tctcgatgtc gagctgcgtg aactcgggct ggcgatcggc
      721 ccgcaggtct tcatcgcgga aacatttcac gatctggtag tagcggtcga agcccgcgac
      781 catgaggagc tgcttgaaga tctgcggcga ctgcggcagc gcgaagaact tgccggcgtg
      841 cgtgcgcgac ggcaccaggt agtcgcgcgc gccttcgggc gtcgccttcg tcagcaccgg
      901 cgtttcgatg tccacgaagc ctgcgtcatc gagataagcc cgcatcgcgc gagttatctt
      961 gtgacgcagt cgcaggcgct tcgacatctc ttcgcggcgc aggtcgatgt aacggtactt
     1021 gaggcggacc tcttcgctca cgtgctcatc gagctggaac ggcagcggct cggcacggtt
     1081 cagcgtcacg agttcgcgcg ccagcatctc gaccttgccc gaacccaggt tcgcgttggc
     1141 ggtacccgcg gggcgctcgc gcaccagccc gcgaatcttc accacgaatt cgttgcgcag
     1201 cttctccgcc tcattgaact gggcctggtt atccgggtcg aaaaccacct gcaggaggcc
     1261 ttcgcggtcg cgaaggtcca cgaagatgac gccgccatga tcgcggcggc gatggaccca
     1321 gccggcaacc gtaacctctt gaccgaccag cttttcgttg acctggccgc aatagtgaga
     1381 gcgcatggaa acctggcaaa tgcgatgaaa gggcggcgat gttaccgggt tgctggcggc
     1441 gggggaaagg ctaagcttcg ggacctttgg tgccatgccg gaaagctcag attccatgca
     1501 atcccttcga ttcgcgctac ccgtgctggc cttcctcttt cttgcctcgg gatgttcgaa
     1561 ggacgacgta ctgcagaagt tcgcgtcgaa agaagaacag gccgtcgcct cgggttacat
     1621 cgaaaagctg cgccaggggc agatctcgga tatcgaggcg caactcgatg tcagcctgcg
     1681 caagcccggg gtgggcgaca cgctgacgaa gatgtccgca atggttccga agggcaaacc
     1741 ggactccgtg acgctggtcg gcgcgcggac ccaatactgg aatggcgcaa agagcgtcaa
     1801 cctcacctat caatatcagt tcggcgaaca gtggttcctc atcaattgcg cgtctcacgg
     1861 cgaaggcgcc gcgctacgca tcctgggcct gaccgtcacc ccgatggcgc agtcgctgga
     1921 cgaacagaac aaattcacgc tcgggggaaa aaactgggtg cagtacacgg tgttggcggc
     1981 cgcgatcacc ttcgcgttgc tcagcatcta tgcgttggtg ctgtgcgcca gggacaagag
     2041 cctgcgcaga aagtgggcct gggtactgtt tgttatgttt ggtgtctgta aattcgcggt
     2101 taactggacg accggggaat ggggcttcac gcccctcgcc atccagttgt tcagcagcgg
     2161 tgcagctgcc gatttgtatg gtccgtggat catttccaca tcgctgccgc tcggggcagt
     2221 gtggtatctg ctgcgtcggc gcaggaatca aattccggcg ccgctgtcga atccgatttg
     2281 aaccgttgcc gatagaggtt tccatgacac gttcgatgac cgccctgttt gcctcagtgg
     2341 ccttcttcac gatgggcgct gcagcccacg cccagtcggc caactgggtc gccggaaagc
     2401 actactcagt catcccgacc cagcgcacca acgtgcccgc gggcaaggtc gaggtcatgg
     2461 aggtcttttc gtacggctgc cccgcctgca acggcttcct gcccgtaatg aagaagatca
     2521 aagcctcgct cccggcgaat gcgcagttgg tttacctgcc ggcctcctgg aacaaggcgg
     2581 aagcctggcc tatgttccag cgagcctatc tcaccgctca gtcgttaggt gtcgccgaga
     2641 aagcgcacga cgagatgttc aaggccatct gggcgacggg cgaactcggc gtcaccgaac
     2701 cttccggacg cctcaagaag aacctgccga ccatcgaaaa tgccgctgcc ttttaccagc
     2761 gtgtcgccgg cgtgaagtcc gcggatttcg tcaacgcctc gaagtccttc accgtcgatc
     2821 tcaagatgcg ccaggccgac agccagatca ttgccatgca ggtgagcggc acgcccacac
     2881 tggtggtgaa cggcaagtac cgactgaaca acgacgcgct gaccacggaa cagatggtcg
     2941 acctcgtcaa gttcctcgtg gccaaggaaa gcgcgccggc agcggcggcg cccgccaaga
     3001 aaggctaggc gaccgcggca cggcggcgct tccacaggcg ccgccctaac cacaccagca
     3061 gtgtcagcgc cgcgagcccc gcggcggcga gcgcgagaag gttgcggatg ggattcacca
     3121 cgatgaagtt cgcgtggcct gaataaaacc cgttgaaatc cgcgagcgcg gcgggcagcg
     3181 ccagcacgtc gtcggcagac agaggcagcg cccgttcgtt tggatgatag gtggagccgc
     3241 tcgggtaagc ctcaccgccc acatccagct cgatgtgcgc gccgagcacg tagtccaccg
     3301 ggtgggtctt cacgaaatcc gcgacccgct gcgcgctcgc gcggtaggcg tcgatatcgt
     3361 cgactaacaa ccgtcccggc aacaggaaat cgccggtcag cagcagcttc gtattgcggt
     3421 cgtagaaaac gatgtgatcc gggtgatggc ccggcgccgg tatcaggtcg acgattcggc
     3481 cgccgaggtc gatctgcccg aggccgttgg gccagtccgc gagcccgagg aattttctca
     3541 gcggttcgct ttcgatgggc gcgatctcga cccctgacaa cttggcaaac tccgcatcac
     3601 ccgcgcggtg gtccaggtgg ccatgcgtat gcaccacgag cagcgggcgc tgatcggctc
     3661 catcggtttg aagcatgttc tccacgacga atgccgtgcg cccggcattc gggccgtcga
     3721 ccgcgcccgt gtcgacgagc aaggaccgct ccttgccgac cagcagatag atcaggttgg
     3781 cttcgtagtc gacacacggg ttctggcgaa tgacgtgcgt gtcgctcgaa tagtgatgcg
     3841 cttcgatcac ttcgttgctt ttggatgccg agcaatccgc ggcacccgcg ttccacggca
     3901 tgcgcagcgt atgcgccccc acgagaggat tcgtgccggg ctcttgcgcc tgggccggca
     3961 gcatgagcag cgcgaacagc ggaaggcaga gcaactgttt atgcatgccg taaccctagc
     4021 ggctggcgct ggagatgtga agccacatcc cggtgacggg acgagcgact cacgacgcct
     4081 agctacgcag ataccacgcg tagtcgagct cgctcacttc gcccgaaaac cgtgcgcact
     4141 cctggttctt gatggcgagg aagatcttga ggaactcccg gcccagcgtt tcttcgagga
     4201 attccgactt cgcggcgagc ttgagcgcct cgggccagct acccggcagc gtgcgttcgc
     4261 cggcctgggc gtagccattg ccgtccacgg ccggacccgg atcgagacgc tcacgcatgc
     4321 catgcgcggc cgcaccgagc accgtcgcca ccgccaggta gaggttggcg tcggcgccac
     4381 agatgcggtg ttcgacatgg cgcgaaatcg cgggtcctgc cggcacgcgc agcgacacgg
     4441 agcgattgtt gatgccccag atcggcgcaa tgggcgcgta actctcgctg acgaagcggc
     4501 gataggaatt cgcgttgggc gcgaacaccg ccatggactc cgccatgctg tctttcatgc
     4561 cgccgatggc ctggcgcaat tcgggggttc cggcggggtc gtcgctggcg aagaggttgg
     4621 cgccattcgt gtcgttcatg ctcacgtgca catgcagccc gctgcccgcg cgttcggtga
     4681 acggcttggc catgaagcag gcgatgaggc cgtgtttctg cgccacgccg cggagcacgc
     4741 gtttgaacat cacggcttcg tcgacggcgc gcagcgcatc ggcgcggtga tgcagcgtga
     4801 tttcgaactg cccgggcgaa tactccgaca tcaacgtctc ggcgggcagc ccctgcgcca
     4861 cggccgcggt gtagacgtca ttgaacatcg gcgcgagatc gtcgagccgc tgcaggctgt
     4921 aggcgtctat cttctggcgt tcgctcgccc gtccgccacc cgcgggtacg agacggccgc
     4981 cggcctcttc cttcaacaga tagaactcga gttcgcacgc gaccacgggc gtgagccccg
     5041 tgcgcgcgaa ccgctccacc acgcgcacca gcgcatgacg cggatccgcc gccgcgggaa
     5101 gcccctgcgc gtcgaacatc gtgagcatga gctggccggt gggcgcagcg agcctcggcg
     5161 caggcttcaa actattcgcg atcgggcgcg cggtcatgtc ggcgtcgccc gtgtcccaga
     5221 cgaggccggt gtcgtcgacg tccttgccag tgatgtcgag cccgaggatc gaccccgcga
     5281 cctggcggcc ggagtcgaag atgcgctcca gttcttcacg gcgcacggcc ttgccgcgca
     5341 gcacgcccga cggatcggtg atcatgatct gcacggcgtg gatgtccgga tgcgcggcga
     5401 ggaaggccgc gagctccgcc ttgtccgcga tctgggtgtg cgcgctcaag acggactgcc
     5461 cacttcgcgc aggcaggcgt gcaggtgctt gagcagcgtg tccgcgtgtg acgtcgtggt
     5521 cacgggcgaa cacagcatca tgttgtggaa cggcgtcagc agaacgccgc ggttcagcag
     5581 atagagatga atggcgtgct ccagctccgg ccaggcggag cgttcggagt ccacaccgtt
     5641 gcgcgcgggc ttgccgaatc cgaattcgag gcgagcgccc acgcgcgcca cgtgccacga
     5701 caaagtctgg gccgcgaaca tgcgccccag cccttcggac aggtaggacg cgcagtcgtg
     5761 catggcctgg tagttagcgg gcgtcatgag ctcgtcgagg ctggcctcga ggcaggccat
     5821 cgccagcgcg tttgccgaca gcgtggtgcc catgccggag tggccgccac cgcgatcctc
     5881 ctgcacgcgg cgcatgcccg cctccacttc cgtcgtgaac ccaaacaccg cgcagggcat
     5941 gccgccggca atcgccttgc cgcaaaccca gatgtccggt tcgagattct gtgcgcgtgc
     6001 ataacc
//
LOCUS       DATDEU010000047         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2050762, whole genome shotgun sequence.
ACCESSION   DATDEU010000047 DATDEU010000000
VERSION     DATDEU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050762"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /gene="ispH"
                     /locus_tag="VIV63_01000"
     CDS             complement(<1..551)
                     /gene="ispH"
                     /locus_tag="VIV63_01000"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017387043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY6123199.1"
                     /translation="MKILLANPRGFCAGVDRAIDIVERAIGMFGAPIYVRHEVVHNVH
                     VVERLRQLGAVFVDELDQVPDGATVIFSAHGVPAAVENEASRRGLTVFDATCPLVTKV
                     HMEVARYAREGRDVVLIGHAGHPEVEGTMGRFDTRGGGRMHLIENVEQARKLEVREPE
                     KLAFVTQTTLSVDDTAAIVNTLRA"
     gene            complement(548..1048)
                     /gene="lspA"
                     /locus_tag="VIV63_01005"
     CDS             complement(548..1048)
                     /gene="lspA"
                     /locus_tag="VIV63_01005"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY6123200.1"
                     /translation="MNIPVRASGIRWLWISVIVIAVDQLTKLWIERTMVLGDGFSVLP
                     VLDIVRAHNTGAAFSFLADAGGWQRWAFSALAVGVSIALVFWLRKLALSTQALLAFAL
                     SLILGGAVGNVLDRIEHGYVVDFVHAHWGSAYFPAFNVADAAISIGAVLVILDAILEG
                     RRNKSA"
     gene            complement(1066..>2202)
                     /locus_tag="VIV63_01010"
     CDS             complement(1066..>2202)
                     /locus_tag="VIV63_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY6123201.1"
                     /translation="EALYSRAPYKGVLTHGFTVDDKGRKMSKSLGNVILPQKVMSSLG
                     ADVMRLWIAATDYANEMGVSDEILKRISDSYRRMRNTVRFLLGNLAGFDPATDALPVD
                     QMVAIDRWALARTAALHEEIVEAYRKYEYHLIYQKIHNFCVVDLGGFYLDILKDRLYT
                     TPKKSVARRSAQTALYHIAEAMVRWLAPILSFTAEEIWSYLPGKRSESVFLETWYQLP
                     GFPSSPAAPSTPMPTPPPTPDNIDWNALIALKTAVGQELEKLRVAGTIGSPLDAEVEV
                     FCKDEFLERFKSLGEELRFLLIVSNAQVKRVSNAAGPPVGAIKVAEVAKEGGLWIRVQ
                     ATPAPKCERCWHHRPEVGSNAEHPTICGRCVDNVTGAGESRTFV"
BASE COUNT          408 a          719 c          696 g          379 t
ORIGIN      
        1 gcccgcagcg tattcactat ggccgccgtg tcgtccaccg agagcgtggt ctgcgtgacg
       61 aaggccagct tctcgggctc gcgcacctcg agcttgcgcg cctgctcgac gttctcgatg
      121 agatgcatgc ggccgccacc gcgggtgtcg aaacggccca tggtgccttc gacttccgga
      181 tggccggcgt gaccgatgag caccacgtcc cgtccctcgc gcgcatatcg cgcgacttcc
      241 atgtgcactt tggtgacgag tggacaggtc gcgtcgaaca cggtgaggcc gcgccgcgac
      301 gcttcgttct cgacggcggc gggcacgcca tgcgccgaga agatgaccgt ggcgccatcg
      361 ggcacctggt cgagctcgtc gacgaacacc gcgccgagct gccgcaggcg ctcgaccacg
      421 tgtacgttgt gcaccacctc gtggcgcacg tagataggcg cgccgaacat gccgatggcg
      481 cgctccacga tgtcgatcgc gcggtcgacg ccagcgcaga agccacgcgg attggcgagc
      541 agtatcttca tgcgctcttg ttgcgacggc cttccaggat cgcgtcgagg atcacgagaa
      601 ccgcgccgat gctgatggcg gcatcggcga cgttgaatgc ggggaaatac gcagagcccc
      661 agtgcgcgtg cacgaagtcc accacgtagc cgtgttcgat gcgatcgagc acgttgccga
      721 cggcgccgcc gagaatgagc gagagcgcga aggccagcag cgcttgcgtg ctcagcgcca
      781 gcttgcgcag ccagaacacc agcgcaatgg aaacgcccac ggccagcgcc gaaaacgccc
      841 agcgctgcca accgccggcg tcggccagga agctgaatgc cgcgccagtg ttgtgtgcgc
      901 gaacgatgtc gagcacgggc agcacgctga aaccgtcgcc cagcaccatg gtgcgctcga
      961 tccacaactt ggtgagctga tccacggcga tcacgatcac cgagatccac aaccagcgga
     1021 tgccactcgc ccggaccgga atgttcacag cttctacgcc ttcgttcata cgaatgtccg
     1081 agactcgccc gctccggtta cgttgtccac acagcggcca cagatcgtcg gatgttccgc
     1141 attcgaaccg acttcggggc gatgatgcca gcagcgctcg cacttgggtg ccggcgtcgc
     1201 ctgcacgcgg atccagagcc cgccttcctt ggccacttcg gcaaccttga tggcgcccac
     1261 aggagggccc gcggcgttac tgacacgttt cacctgcgca ttcgaaacga tcagcaggaa
     1321 ccgcagttct tcgccgagcg acttgaaacg ctccaggaac tcgtccttgc agaacacctc
     1381 gacttcggcg tcgagcggcg aaccgatggt gccggccacg cgcagcttct cgagctcctg
     1441 gccgacggcg gtcttgagcg cgatgagcgc gttccagtcg atgttgtccg gcgtcggtgg
     1501 aggcgttggc attggcgttg agggagcagc cggcgaagat ggaaagcccg gcaactgata
     1561 ccaggtctcg aggaacacgg actcgctgcg cttgcccggc aggtagctcc agatctcctc
     1621 cgcggtgaat gacaagatag gcgctaacca ccgcaccatc gcttcggcga tgtgataaag
     1681 cgccgtctgc gccgaacgcc gcgccacgct cttcttcggc gtggtgtaga gccggtcctt
     1741 gaggatgtcg agatagaagc cgccgagatc cacgacgcag aagttgtgga tcttctgata
     1801 gatgaggtgg tactcgtact tgcgatacgc ctccacgatt tcctcgtgca acgctgcggt
     1861 gcgcgccagc gcccagcggt cgatggcgac catctggtcc acgggcagcg catccgtcgc
     1921 cggatcgaaa cccgcgagat tgcccagcag gaaacgcacc gtgttgcgca tgcggcgata
     1981 cgaatccgag atgcgcttga ggatttcatc cgacacgccc atttcgttgg cgtagtcggt
     2041 ggcggcgatc cacagccgca tcacatcggc gccgagcgac gacatgacct tctgcggaag
     2101 gatcacgttg cccagcgact tggacatctt gcggcccttg tcgtcgacgg tgaacccgtg
     2161 cgtgagcacg cccttgtagg gcgcgcgcga atacagcgct tc
//
LOCUS       DATDEU010000048         7684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_299960, whole genome shotgun sequence.
ACCESSION   DATDEU010000048 DATDEU010000000
VERSION     DATDEU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299960"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1522)
                     /locus_tag="VIV63_01015"
     CDS             complement(32..1522)
                     /locus_tag="VIV63_01015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6123202.1"
                     /translation="MKRRQFIHAALAAVPAAVLPKFALATTSAADLPDVEAITNQGKQ
                     IILKNSDVKDFAARLRGELLLRESAGYDSARRVWNGAFDRHPALIARCSGPADVMEAV
                     KFAASNQLLLSVRGGGHSLSGQSVCEKGLMIDLSQMNSARVDPVRKLATIEGGALLGA
                     LDREALAHGLATTAGTVSHTGVGGLTLGGGFGRLGRRFGLACDNVRAIDVVTADGKFV
                     TASAKENQELFWGLRGGGGNFGVATSFDFQLHPVDPLMIGGELVYSFADAPAVLKYMF
                     EYGPEVPEELNMDVSLVRLPNDQRVLSIDVCYSGSAAGADKALAGMRSIRKPIADMVK
                     PTPYIKLQQSGDEGTAHGKKYYIKGGFVQQSSEALIDVMMATVAEAKLPVVQVVSVQQ
                     AGGAIARVAPKATAFAQRAIQYNMFVLAVWEDPALSDPVGQWARSAWKNIEPHTHGFY
                     VNEFNEDGGRMKATYGVNYERLVALKTKVDPGNLFRLNANVAPKSA"
     gene            complement(1718..2035)
                     /locus_tag="VIV63_01020"
     CDS             complement(1718..2035)
                     /locus_tag="VIV63_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEY6123203.1"
                     /translation="MNIEAEVLWLDEHRTVSLHELVELSGLLETELLELVHSGVIPSS
                     DSPGAGYVFSARVITVARTACRLRDDFELDTRGLAVALRLLERVRDLEEEVARLRAEL
                     PGS"
     gene            complement(2032..2973)
                     /locus_tag="VIV63_01025"
     CDS             complement(2032..2973)
                     /locus_tag="VIV63_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY6123204.1"
                     /translation="MKYTDYYAALGVERGASADVIRKAYRRLAQKHHPDVSKEPGAED
                     RFKEIAEAYQTLKDPEKRAAYDALGSRPSGEEFRPPPGWARDHGGSGAEQFSFDDADF
                     SDLFSRFGGGGARRSGGPLPGRDFEVPVEISLEDAFRGTTLSLSLSMPEYDATGNLRR
                     VPHNVKARVAPGAVDGQRLRLPGKGGKGFQGGRDGDLYLDITLKPHRLYRATGHDLYL
                     DLPLAPWEAALGATVEIPTLSGAVSLKVPAGTSAARKLRLAGRGLPKPRGGAGDLIAV
                     AQIVVPAEVGERERELYQQLAEAGSFDPRRHFAEENA"
     gene            complement(2984..3772)
                     /locus_tag="VIV63_01030"
     CDS             complement(2984..3772)
                     /locus_tag="VIV63_01030"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEY6123205.1"
                     /translation="MKRTSIFVLGALLAQAASAGVYVELVDRELANGAVTEEKLSQKI
                     YMQDGSGRFVDAEGRATLIKGDTLYILDDADKSYVAFDKATMEVLAKKINEAMAQARE
                     QLAKLPPDQRAQMEQMMPGVANADGKWTVEAVDTGKSDKVDGRACHLWDIKRNGELDD
                     QWCVVPYSALPGKENFSAVFANFAKVFEEMAKSVPMLSGMMSNEFGAQSKVNGFPVRS
                     RSYENGRLSGSEQLMRVWREEAIPASMFNIPAGYQQKQMSLGDE"
     gene            3831..4712
                     /locus_tag="VIV63_01035"
     CDS             3831..4712
                     /locus_tag="VIV63_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6123206.1"
                     /translation="MEERRKPILGRVVPGVTPTLQRRLRRLFAFLQWLSVPLASKVAF
                     RLFLTPPRRKVEAGDVPIVAQAERATLRCGADKFTVWHWGKDGPCVVLLHGWGSHAAR
                     FANFVAPLRAAGFSVIGIDAPAHGTSPGKLSDLPRFRDSLAEVLRQHSPVHAVVGHSL
                     GGGAVLTVLAETADYHPKKICLFGVPSDMDYILGSFAMMLGLKYAARAHLRERFAAHF
                     GRPASAISVAAAAASVRIPVLIVHDEEDNVAPIDQGQALAAAIPHAMIWHTRGRGHSG
                     ALRDAETIKRVVSCLAE"
     gene            complement(4717..5385)
                     /gene="hxpB"
                     /locus_tag="VIV63_01040"
     CDS             complement(4717..5385)
                     /gene="hxpB"
                     /locus_tag="VIV63_01040"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HEY6123207.1"
                     /translation="MFRAAIFDMDGLLIDSEGFWKRAERDVFGSVGIEINDEMAATTA
                     PMTTRQVAEHWYGVRPWHERTIEDMEAAVISRVADQIRSTTAAMPGVHEILALCANLG
                     WRVGLASNSPAVLCQLVLSQLGIASAFHAVVSADFVDEGKPHPAIYLHAARLLGVPPN
                     ECIAFEDSATGVRAARAAGMSVVAVPSAGQVFPVGPSAPHVLLNALHEFARDHAQELW
                     AARG"
     gene            complement(5406..6302)
                     /locus_tag="VIV63_01045"
     CDS             complement(5406..6302)
                     /locus_tag="VIV63_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123208.1"
                     /translation="MRLGLILLLCLLNTGCALWRRDRAEPVENGPRESKAEPVLKPSE
                     ALKPEALEIASPVSDQFFMRGTYYQGEVATVLRVDSTGATEPDGTVLSGEDDLGLDDV
                     VDQARLEFDIRMGERNHVRIDYFKLNRYAETNLPRDIEFGDFTFDEGDRFSSKLDWRM
                     LSLTYTYSLFRSERFEAGVGLGLHIIETSAEGSEPGTLNREKDSEAGIFPTIAVNGAF
                     RISKRWAITARGQTFNAHPEGFDGTLSDYHVDVQYRWRRNFAVGIGYSQFEVDLDVVD
                     ADQPFLFQMETSGPEIFFRASF"
     gene            complement(6441..>7684)
                     /gene="rho"
                     /locus_tag="VIV63_01050"
     CDS             complement(6441..>7684)
                     /gene="rho"
                     /locus_tag="VIV63_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY6123209.1"
                     /translation="LKAKPIYELVKMAETMGVEGMARSRKQDIIFGILKAHSRLGENI
                     YGDGVLEILQDGFGFLRSADGSYLAGPDDIYVSPSQIRRFNLRTGDTISGLIRPPKDG
                     ERYFALLKVGEINFDSPDSVRNKVLFENLTPFHPTKRLKLERGNGSTEDLTARAIDLV
                     SPIGKGQRGLIVSPPKAGKTMLLQNIATAITANHPEVHLIVLLIDERPEEVTEMQRTV
                     KGEVVSSTFDEPAARHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPSS
                     GKVLTGGVDANALQRPKRFFGAARNIEEGGSLTILATALIDTGSKMDDVIYEEFKGTG
                     NCEIHLDRRIAEKRVFPAMNINRSGTRKEELITDPGELAKIWILRKLLHPMDELAAVE
                     FLLEKMKDTKTNSEFFVAMKR"
BASE COUNT         1299 a         2505 c         2441 g         1439 t
ORIGIN      
        1 gttccgcgct cgcggaacct cccgcttttc ctcaggcgct cttcggcgcc acgttcgcgt
       61 tcaagcggaa caggttgcca gggtcgacct tcgtcttcag cgcgaccagc cgttcgtagt
      121 tcacaccgta ggtcgccttc atgcggccgc catcctcgtt gaactcgttg acgtagaagc
      181 cgtgggtatg cggctcgatg ttcttccacg cgctgcgcgc ccactgcccc accggatcgc
      241 tgagcgccgg atcttcccag accgcgagca cgaacatgtt gtactggatc gcgcgttgcg
      301 cgaatgcggt ggccttgggc gcgacacgcg cgatggcgcc acccgcctgc tgcacgctga
      361 ccacctgcac caccggcaac ttcgcttccg ccaccgtcgc catcatcacg tcgatgagcg
      421 cttcgctcga ctgctgcacg aagccaccct tgatgtagta cttcttgccg tgcgcggtgc
      481 cttcgtcacc ggactgctgc agcttgatat acggcgtcgg cttgaccatg tcggcgatgg
      541 gcttgcggat ggaccgcatg cccgcgagcg ccttgtcggc gcccgcagcg ctgccgctgt
      601 aacagacgtc gatcgacagc acgcgctggt cgttgggcaa ccggacgagt gacacgtcca
      661 tgttgagctc ttccggaact tccggaccgt actcgaacat gtacttgagc acggccggcg
      721 cgtccgcgaa cgagtagacg agttcgccgc cgatcatcag gggatcgacc gggtgcagct
      781 ggaaatcgaa cgacgttgcg acaccgaagt tgccgccgcc gccacgcaat ccccagaaca
      841 actcctggtt ttccttcgcg ctggcggtga cgaatttgcc gtcggccgtg accacatcga
      901 tggcgcgcac gttgtcgcag gcgagtccga agcggcggcc gagccggccg aagccgccac
      961 ccagcgtgag gccgccgacg cccgtgtgcg agacggtacc ggcggtcgtc gccaggccat
     1021 gcgccagcgc ttcgcggtcg agcgcgccta acaaggcgcc gccttcgatg gtcgcgagtt
     1081 tcctcacggg atcgacacgc gcgctgttca tctgcgacag gtcgatcatc agaccctttt
     1141 cgcaaacaga ctggcccgac aggctgtggc caccgccacg caccgagagc agcaactggt
     1201 tcgacgccgc gaacttcacg gcctccatga catcggccgg gccactgcaa cgcgcgatca
     1261 gcgccggatg acgatcgaac gcaccgttcc agacgcgacg cgcgctgtcg tagccggcgc
     1321 tctcacgcag cagcaattcg ccacgcaagc gggcggcgaa atccttgacg tccgagttct
     1381 tgaggatgat ctgcttgccc tggttcgtta tcgcctcgac gtcgggcaga tccgccgcgg
     1441 acgtggtcgc gagcgcaaat ttcggaagga ccgcagcagg caccgcggcg agcgcggcgt
     1501 ggatgaattg acggcgtttc atgatccagg cgaccttgtg gttgaaatgt tgcggtgcgc
     1561 actctacgcc tgcgtttgac aataaatgca agcgcgaagg cggctgaaac gcgattttga
     1621 gaggtattac aggtgactga gcccgacctg gaatcgttct caggaaatcc gacgggctgc
     1681 cgccggggac tttcggcacc gctgaaccgc cgcgtgccta actaccgggc agctccgcgc
     1741 gcaggcgcgc gacttcctct tcaaggtcgc gcacacgctc cagcagccgc agcgccaccg
     1801 ccaggccgcg cgtatccagt tcgaagtcgt cgcgcaggcg gcaggccgtg cgggccacgg
     1861 tgatcacgcg tgcactgaac acgtagccgg cgcccggcga gtcgctcgat gggatgacgc
     1921 cgctgtgcac gagctcgagc agctctgttt cgagcaggcc cgacaactcg accagttcgt
     1981 gcagactgac ggtgcgatgt tcatcgagcc acaacacttc ggcttcgatg ttcatgcatt
     2041 ttcctcggcg aaatgccgcc gcgggtcgaa actgcccgcc tcggcgagct gctgatacaa
     2101 ctcgcgctcc cgttcgccta cctcggccgg caccacgatc tgcgccaccg caatgagatc
     2161 gcctgcaccg cctcgcggct tcggcagccc gcggccggcg agcctcagtt tgcgtgcggc
     2221 cgaggttccg gccggcacct tgaggctcac cgccccggac agcgtgggga tttccaccgt
     2281 ggcgccgagc gcggcttccc acggcgcgag tggcagatcc agatagaggt cgtgcccggt
     2341 ggcgcgatac aggcgatgcg gcttcaacgt gatgtcgagg tacaggtcgc cgtcgcggcc
     2401 gccctggaag cctttgccgc ccttgccggg caggcgcagg cgctgaccgt cgacagctcc
     2461 gggcgcgaca cgcgccttca cgttgtgagg gacgcgccga aggtttccgg tcgcgtcgta
     2521 ttccggcatc gacagcgaca atgacaacgt ggtgccgcga aatgcgtctt cgaggctgat
     2581 ttccaccggc acctcgaagt cccggcccgg caggggaccg ccacttcggc gcgcgcctcc
     2641 gccgccgaaa cgcgagaaca gatcggaaaa atccgcgtcg tcgaaggaga attgctcggc
     2701 ccccgagccg ccatggtcgc gcgcccagcc aggcggcggc cggaactcct cgccggaagg
     2761 acggctgccg agcgcgtcgt acgccgcgcg tttctcggga tctttcagcg tctggtacgc
     2821 ctcggcgatt tccttgaagc gatcttccgc tcccggctct ttcgagacat cggggtgatg
     2881 cttctgcgca aggcgccggt aggccttcct gatcacatcg gctgaagcgc cgcgctcgac
     2941 gcccagcgcg gcgtagtagt ccgtgtattt catcgcccct cgatcactcg tctcccaggg
     3001 acatctgctt ttgctgatag cccgcgggaa tgttgaacat cgaggccggg atggcttctt
     3061 cccgccacac gcgcatgagc tgctcggaac cactcagcct gccgttttca taactgcgcg
     3121 agcgcacggg gaaaccgttc accttggact gcgcgccgaa ttcgttggac atcatgccgg
     3181 acaacatggg gacggatttc gccatttcct cgaacacctt ggcgaaattc gcgaacaccg
     3241 cggagaagtt ttccttgccg ggcaatgccg agtacgggac cacgcaccat tggtcgtcga
     3301 gctcaccgtt tcgcttgatg tcccagagat ggcaggcgcg cccatctacc ttgtcggact
     3361 tgccggtgtc gacggcttcc acggtccact tgccatcggc attcgccacg ccgggcatca
     3421 tctgctccat ctgcgcgcgc tgatccggcg gcagtttcgc gagctgttcc ctggcctgcg
     3481 ccatcgcctc gttgatcttc ttcgcgagca cttccatggt cgccttgtcg aacgcgacgt
     3541 aactcttgtc cgcgtcatcg aggatgtaca gcgtgtcgcc cttgatgaga gtggcgcggc
     3601 cttccgcgtc gacgaagcgg ccgctgccgt cctgcatgta aatcttctgc gagagtttct
     3661 cttcggtcac cgcgccattc gcgagttcgc gatcgaccag ctcgacatac acgccggcgc
     3721 tcgccgcttg tgccaggagc gcccccagga cgaagatgga tgtgcgtttc atggagcccc
     3781 tcatggtttg tgtcaggact ctacaccgca cgatatagac ttcaaaccgc atggaagagc
     3841 gccgcaagcc aatcctggga cgcgtggttc cgggcgtcac gcccaccctg cagcgacggc
     3901 tgcggcgtct gttcgccttt ctgcaatggc tcagcgttcc gctcgccagc aaggtcgcgt
     3961 ttcgcctgtt cctcacgccg ccacgccgca aggtcgaggc cggcgatgtg ccgatcgtcg
     4021 cgcaggcgga acgcgccacg ctgcgttgcg gagccgacaa attcaccgtg tggcactggg
     4081 gcaaggacgg cccctgcgtg gtgctgctgc acggctgggg aagtcacgct gcgcgtttcg
     4141 ccaattttgt cgcgccgctg cgcgcggcag gattttccgt gatcggcatc gatgcgcccg
     4201 cgcatggcac atccccgggc aagctttcgg acctgccgag atttcgcgac agcctcgccg
     4261 aagtgctgcg acaacattcc ccggtccacg cggtagtggg ccattcgctc ggcgggggtg
     4321 ccgtgctcac cgtgctggcg gaaaccgccg actaccatcc gaaaaagatc tgcctcttcg
     4381 gcgtgccgtc ggatatggac tacatcctcg gttcgttcgc catgatgtta ggcctcaagt
     4441 acgcggcgcg cgctcatctg cgtgaacgct tcgcggcgca tttcggccgc cccgcttccg
     4501 ccatttccgt ggccgccgcc gcggcgtcgg tacgcattcc ggtcctcatc gttcacgacg
     4561 aagaagacaa cgttgcgccc atcgaccagg ggcaggcgct cgcggccgcc attccgcacg
     4621 ccatgatctg gcacacgcga ggacgggggc acagcggcgc gttgcgcgat gccgagacca
     4681 tcaaacgcgt ggtgagctgc ctcgccgagt aacgggtcag ccgcgcgcgg cccacagttc
     4741 ctgcgcgtga tcgcgcgcga actcgtgcaa cgcgttgagg agcacgtgcg gagcggatgg
     4801 cccgacggga aatacctgcc ccgcggacgg caccgcgacc acgctcatgc cggcggcgcg
     4861 cgcggcccgg actccggtcg ccgagtcctc gaaggcgatg cattcattcg gtggcacgcc
     4921 gagcagacgg gccgcgtgta gatagatggc cgggtgtggc ttgccttcgt cgacgaaatc
     4981 cgcggacacg accgcgtgga acgcactggc gatgccgagc tggctcagca cgagctggca
     5041 gaggacggcc ggagaattcg acgcgaggcc gacacgccag ccgagattcg cgcacagcgc
     5101 gagtatttcg tggacgccgg gcatggcggc ggtggtcgaa cggatctggt cggcgacgcg
     5161 cgagatcacc gcggcttcca tgtcttcgat ggttcgttca tgccatggcc gcacgccata
     5221 ccagtgctcg gcgacctggc gtgtcgtcat cggcgccgtg gtcgcggcca tctcgtcgtt
     5281 gatttcgatg cccaccgaac cgaagacatc acgttcggcc cgcttccaga aaccctcgga
     5341 gtcgatcagc agaccgtcca tgtcgaagat cgcggcgcgg aacatgacgc gcgtttgctc
     5401 ggaggtcaga agcttgcgcg gaagaagatc tcggggccgc tggtttccat ctggaacagg
     5461 aacggctggt ccgcgtccac gacgtcgaga tcgacctcga actggctgta gccgataccg
     5521 acggcgaaat tccggcgcca gcggtactgc acgtcgacgt ggtagtcgga cagcgtgccg
     5581 tcgaagcctt ccggatgcgc attgaacgtc tggccgcgcg cggtgatcgc ccagcgcttg
     5641 gagatgcgga atgcgccatt caccgcgatg gtggggaaga tgccggcttc ggaatccttt
     5701 tcccgattga gcgtgccggg ctcgctgcct tcggcgctcg tctcgatgat gtgcagcccc
     5761 aggccaactc cggcctcgaa gcgttcggac ctgaacagcg aataggtgta ggtcagcgac
     5821 agcattcgcc agtcgagctt ggaactgaac cggtcacctt cgtcgaaagt gaaatcgccg
     5881 aactcgatgt cgcgcggcaa attcgtttcg gcgtagcgat tcaacttgaa atagtcgatg
     5941 cgcacgtgat tgcgctcgcc catgcggatg tcgaattcca agcgcgcctg gtcaacgacg
     6001 tcgtcgagcc cgaggtcgtc ttcgccgctc agcacggtgc cgtcgggttc ggtcgcgccg
     6061 gtggagtcga cgcgcaacac ggtggcgacc tcaccctggt agtaggtgcc gcgcatgaag
     6121 aattgatcgg aaacggggct ggcgatttcg agcgcttcgg gcttgagcgc ctcgctgggc
     6181 ttgagcacgg gttcggcctt gctctcgcgc gggccgttct cgaccggctc cgcacggtcg
     6241 cgacgccaca gcgcgcagcc ggtgttcaga agacacagaa gcaggatcag cccaaggcgc
     6301 attcgaagct ccgcaaacag ccctggatac agggccgcgc catgatctaa cagcttggga
     6361 atgggcgcca gcgtcgcggt caagcgacgc gcccggtgtg agccacgtca caccgggcgg
     6421 cgccagagta tcgactgaag ctatcgcttc atggccacga agaattccga attggtcttc
     6481 gtatccttca tcttttcgag caggaactcg accgcggcca gctcatccat cggatgcagc
     6541 agcttgcgca ggatccagat cttggcgagc tcgcccggat cggtaatcaa ctcttccttg
     6601 cgcgtgccgg accgattgat attcatggcg gggaacacgc gcttttcagc gatgcgccgg
     6661 tcgagatgta tttcgcagtt gcccgtgccc ttgaactctt cgtagatcac gtcgtccatc
     6721 ttcgagccgg tgtcgatgag cgcggtggcc aggatagtta gactgccgcc ctcctcgatg
     6781 ttgcgcgcgg cgccgaagaa gcgcttgggg cgctgcaggg cgttcgcgtc cacgccgccg
     6841 gtcaacacct tgccggagct cggcaccacg gtgttgtagg cgcgcgcgag gcgtgtgatg
     6901 gagtcgagga ggatcaccac gtctttcttg tgctcgacca ggcgcttggc cttctcgatc
     6961 accatttccg cgacctgcac gtggcgggca gcgggttcgt cgaacgtgga cgacaccact
     7021 tcgcccttca cggtgcgctg catctcggtc acttcttccg ggcgttcatc aatcagcaga
     7081 acgatcagat gcacttcggg gtggttagcc gtgatggcgg tggcgatgtt ctgcagcagc
     7141 atggtcttgc cggctttcgg cggcgagacg atgagaccgc gctggccctt gccgatgggc
     7201 gagaccaggt cgatggcgcg tgcggtgaga tcttcggtcg aaccattgcc ccgctcgagc
     7261 ttgagccgct tggtgggatg gaacggcgtc aggttctcga acagcacctt gttgcgcacg
     7321 ctgtccggcg aatcgaaatt gatctcgccg accttgagca atgcgaaata acgctcgcca
     7381 tctttcggcg ggcggattag gcccgagatg gtgtcgccgg tgcgcaggtt gaagcggcgg
     7441 atctggctgg gcgacacgta gatgtcgtcc ggacccgcga gatacgagcc gtcggccgaa
     7501 cgcaggaagc cgaagccgtc ctgcaggatt tcgagcacgc cgtcgccgta gatgttctcg
     7561 ccaaggcgag agtgcgcctt gaggattccg aagatgatgt cctgcttgcg cgagcgggcc
     7621 atgccctcga cgcccatggt ctcggccatc ttgaccagct catagatggg cttggccttg
     7681 agca
//
LOCUS       DATDEU010000049         2926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1467339, whole genome shotgun sequence.
ACCESSION   DATDEU010000049 DATDEU010000000
VERSION     DATDEU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467339"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VIV63_01055"
     CDS             complement(<1..1214)
                     /locus_tag="VIV63_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEY6123210.1"
                     /translation="MDYASTLLQLLPSARRLPGLRELINSIEAYLPPEQVERVRSAAE
                     FGAEAHEGQKRLSGEPYIAHPLAAAQILADLHLDPDTIIAAILHDVIEDTPISKEELS
                     QKFGAPVAEIVDGVTKLDQIQFKSRAEAQAESFRKMVLAMVRDLRVILVKLADRTHNM
                     RTLDAMAPARRRAVARETLDIYAPIAGRLGLYNMKLELEDLGFEAAFPHRYKVLDRAL
                     KKARGNQKQFLAKIADTMRVALVKADFKADVSTREKHLYSIYSKMLRKAAPLSEIVDV
                     FGLRIVVDRADTCYRALGVVHAAYKPMPGRFKDYIAIPRVNGYQSLHTTLFGPNGIPI
                     EVQIRTEDMHRVAEAGIAAHWKYKVNETEGDQDARAREWLANLVDMQEGGSSEDFLES
                     VKVDLFPDKVYV"
     gene            complement(1359..1553)
                     /gene="rpoZ"
                     /locus_tag="VIV63_01060"
                     /pseudo
     CDS             complement(1359..1553)
                     /gene="rpoZ"
                     /locus_tag="VIV63_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            complement(1661..2293)
                     /gene="gmk"
                     /locus_tag="VIV63_01065"
     CDS             complement(1661..2293)
                     /gene="gmk"
                     /locus_tag="VIV63_01065"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY6123211.1"
                     /translation="MNAAAARRRGSLFVIAAPSGAGKTSLVKAILARDPALRVSVSHT
                     TRKQREKEIPGRDYNFVDIAEFTRLRDAHEFLEHAQVFDNFYGTGRAQVEALRNAGHD
                     VILEIDWQGAQQVRKAAPDCKTVFILPPSRVELESRLRNRKTDSDQVIERRLRDAVAD
                     MSHYAEFDYVIVNDDFEAAVAHLLEILRGGGTEYVAKRADLAPLLADLLG"
     gene            complement(2290..>2926)
                     /locus_tag="VIV63_01070"
     CDS             complement(2290..>2926)
                     /locus_tag="VIV63_01070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY6123212.1"
                     /translation="ELRIDEAALSRVAAAVNQVARTVPGSTIDAVEVLRWPGVIETGT
                     QDSEALLVAGRAVFQQTLDELGAMRLREGKRLGELIEQRCAGLAMLVTQVRARLPEIQ
                     NRVRTRLHERVAELLATVDRERVEQEIVLQLQRLDVAEELDRLTGHIEETRRVMSGGE
                     AAGRRLDFLMQELNREANTLASKSQDLDTTRITVDMKVLIEQMREQVQNVE"
BASE COUNT          469 a          905 c          983 g          569 t
ORIGIN      
        1 aacacgtaga ccttgtcggg gaacaggtcg accttgacgc tctcgaggaa gtcctcggag
       61 ctgccgcctt cctgcatgtc gacgagattg gcgagccatt cgcgcgcacg cgcgtcctgg
      121 tcgccttcgg tctcgttgac cttgtatttc cagtgcgcgg cgatgccagc ctcggcgacg
      181 cgatgcatgt cctcggtgcg gatctgcact tcgatcggga tgccgttcgg gccgaacagc
      241 gtcgtgtgca acgactggta gccgttgacg cgcggaatcg cgatgtagtc cttgaagcgg
      301 ccgggcatcg gcttgtacgc cgcgtgcacc acaccgagcg cgcgatagca ggtgtcggcc
      361 ctatctacca cgatgcgcaa tccgaatacg tcgacgatct cggagagcgg cgccgccttg
      421 cgcagcatct tgctgtagat gctgtagaga tgtttctcgc gcgtggagac gtcggccttg
      481 aagtcggcct tcacgagcgc gacacgcatc gtgtccgcga tcttcgcgag gaactgcttc
      541 tggttgccgc gggctttctt cagcgcgcga tccagcacct tgtagcgatg cggaaacgcg
      601 gcctcgaagc cgagatcctc gagctcgagc ttcatgttgt agagcccgag acgcccggca
      661 atgggtgcgt agatgtcgag cgtctcgcgc gccaccgcgc ggcgacgcgc gggcgccatc
      721 gcatcgagcg tgcgcatgtt gtgcgtgcgg tccgcgagct tcacgaggat cacgcgcagg
      781 tcgcgcacca tcgccagcac catcttgcgg aaggattccg cctgcgcttc ggcgcgcgac
      841 ttgaactgga tctggtcgag cttggtgacg ccgtcgacga tctcggccac gggagcgccg
      901 aacttctgcg acagctcctc tttcgagatc ggcgtgtcct cgatgacgtc gtgcaggatc
      961 gccgcgatga tcgtgtcggg gtcgaggtga aggtcggcga ggatctgcgc ggccgccagg
     1021 ggatgcgcga tgtagggttc gccggagagc cgcttctggc cttcatgcgc ttccgcgccg
     1081 aattcagcgg ccgagcggac tcgctcgacc tgctcgggcg gcagataggc ttcgatgctg
     1141 ttgatcagct cgcgcaggcc cggcagtcgc cgggcactgg gcaaaagctg aaggagcgtc
     1201 gacgcgtaat ccacggggcg cgagctcctt tagaggaagt tcaggcggac gatttattcc
     1261 tgaacgtcac gatcgccttg caggccgaat tgcggcgcac ggaacaggga ttggttgtcg
     1321 gcatccggca gcggagcttc gaccggcggc ggatccggct cacggagcat ttccggcgtg
     1381 atgttgcctt cggcaatctc gcgcagggcc actacggtcg gcttgtcgtt ctcccacgga
     1441 acggtgggct cggcgccatt ggcgaggcgg cgcgcacgct gcgcggccag caacacgagc
     1501 tggaagatgt tttcgacgtt cgggaggcag tcttcgacag taattcgggc catggtgtcc
     1561 agagaagcaa gctgcggggg gacgaaagtc tactggaaaa gagccgggat ttgacggttt
     1621 ttgtcccccg tgggcgtgaa gcgcccgggc ggcccggctg tcagccgagc aagtcggcca
     1681 gcaggggcgc gagatcggcc cgtttggcga cgtactccgt tcccccaccc cgcaggatct
     1741 cgagcagatg agccacggcc gcttcgaaat cgtcgttgac gatgacgtag tcgaattccg
     1801 cgtaatgcga catatcggcc acggcgtcgc gcaagcgccg ctcgatgacc tggtcgctgt
     1861 cggtcttgcg gttgcgcagc cggctctcga gctcgacgcg cgaaggcgga aggatgaata
     1921 cggtcttgca gtcaggcgca gccttgcgaa cctgctgtgc tccctgccag tcgatttcca
     1981 ggatcacgtc gtgacccgca ttgcgcagcg cttcgacctg cgcccggccg gtgccgtaga
     2041 agttgtcgaa gacctgagcg tgttcgagga attcgtgggc gtcgcgcagc cgtgtgaatt
     2101 cggcgatatc gacgaagttg tagtcgcgac caggaatctc tttttcccgc tgttttcgcg
     2161 tggtgtgtga caccgatacg cgcaacgcgg gatcccgcgc gaggatggct ttgacgaggc
     2221 tggtcttgcc ggcgcccgag ggcgcggcga tgacgaatag agagccgcgt cgcctggccg
     2281 ctgcggcgtt cattcgacgt tctgtacctg ttcgcgcatc tgctcgatca gcaccttcat
     2341 gtcgaccgtg atgcgcgtgg tgtccagatc ctgcgatttg gaagccagcg tgttcgcttc
     2401 gcggttgagt tcctgcatga ggaagtcgag gcgacggccg gcggcctcgc cgccgctcat
     2461 cacgcgccgt gtttcttcga tatggccggt gagccggtcg agttcttcgg cgacgtcgag
     2521 gcgctgcagc tgcagcacga tttcctgctc cacgcgctcg cgatcgacgg tcgccagcaa
     2581 ttcggcgacg cgttcgtgca ggcgcgtgcg cacgcgattc tggatctccg gcaggcgcgc
     2641 gcgcacctgg gtgaccagca tggcgagccc ggcgcaacgt tgttcgatga gttcgcccag
     2701 gcgcttgcct tcgcgcaggc gcatcgcacc gagttcatcc agcgtctgct ggaacaccgc
     2761 gcggcccgcg actaacaaag cttccgagtc ctgggtgccg gtctcgatga cgccgggcca
     2821 gcgcaatact tccacggcat cgatggtgct gcccggcacc gtgcgcgcca cctggttcac
     2881 ggccgcggca acccgcgaaa gcgccgcttc atcgatgcgc agctca
//
LOCUS       DATDEU010000050         3648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_737834, whole genome shotgun sequence.
ACCESSION   DATDEU010000050 DATDEU010000000
VERSION     DATDEU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737834"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VIV63_01075"
     CDS             complement(<1..203)
                     /locus_tag="VIV63_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123213.1"
                     /translation="MSTKTRVLNVRVVGFSAPERQMLNSIFLLSVAPARQIGYKLWNP
                     EAGVAPDMYLLDGDSDSAVASWQA"
     gene            complement(225..3020)
                     /locus_tag="VIV63_01080"
     CDS             complement(225..3020)
                     /locus_tag="VIV63_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6123214.1"
                     /translation="MRGQDLLTALADELKGLAPELAAAYDSGSPGDDAYVTSVARLSE
                     AAGYMGLAGVQRIAACVLDNLQHLDADDQDARVLVRPFFTDWPRLLEAHLRDANSPAP
                     IGSLIAHFGGGWVPLPLDESALNDLRAELAAANSIGAALDESEQAAPEAPDAADLSLA
                     ISSDVDASLIDAFLHDSPPQAAEVTQSLQGWIADPSQSELLRNAKRAAHTLKGSANIL
                     GIRGVAMLAHRLEDILEICEAESAPPSSMRAQALVSAADCLEQMVATVAGEDSAPENA
                     LGVIELLDAARNNDASDTVRHRALEMPVPVQAAPAAPAAAAAPRTEAKATTRVPTELL
                     DNLVRMVGELTIKIGELDQELKSASGHSRELIERDRAIQKRLYELEHSVDIRGVAARH
                     KLNVVHAASDFDSLEMDQYNEVQSLTRALIEETTDARDLGLGLKDELAGLAGILHQQQ
                     RLMRELQHLTSQTRMAPVETIVPRLQRNVRQTAAATYKQASLVVVGSEILVDAEVLNQ
                     LIDPLLHILRNAVDHGLETPAERESVGKPRAGTITLTVARRGQVVTVQVADDGRGLNL
                     ADIRAKAIERGLLEVDATPSDAEIARLTLLPGFSTRDAVTEISGRGVGLDVVATRLRA
                     LSGSIDIRSEAGAGLSIELRFQASLVATHALFVRDGGQIFGIASHAVRTAVPAVAVKI
                     EREDGVAFARVDDILYPVHELAMLTGMRPATAPERRNLVLVDSESGPIGVLVDAVLEA
                     SELVTRPTGRYLKRIPGVTGIGLTGNGAVIPLLDIAELARTPRDQAMRAAAEARSQAK
                     ELRRSRVLIVDDSMSVRRAVATLLEDHGYDVALARDGLEAVKAMEAARPDALLTDLEM
                     PNMNGLELTAHVRARPELSNLPVIMITSRSLDKHRRQAMASGVNVYLTKPYTDQELLQ
                     HVASALSERVEKRAAAG"
     gene            complement(3042..>3648)
                     /locus_tag="VIV63_01085"
     CDS             complement(3042..>3648)
                     /locus_tag="VIV63_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6123215.1"
                     /translation="MEGIRESIAESEKRIKRLGERSQEITGIVNLINTIAERTHVLAL
                     NASMQAAAAGDAGRGFAVVAEEVQRLAESSRQATQQIAQLVNNIQIETSDTINTVNKT
                     IAQVVSGSEIAARSGAQMRETQQSTASLVELVKRITSSAQVQMKITTELRNRVHQIGA
                     STEKTAAEILLQSEATADLSKSADRLVESVRVFKLPTIRAA"
BASE COUNT          570 a         1126 c         1283 g          669 t
ORIGIN      
        1 gcctgccagc tggcgaccgc gctatcgctg tctccatcta gcaagtacat gtccggcgcg
       61 acgccggcct ccggattcca gagcttgtag ccgatctggc gtgccggagc gaccgacaac
      121 aggaagatcg agttcagcat ctgccgctcg ggagcggaaa agccgacgac tcggacattg
      181 aggacgcggg ttttggtact catttcaatt taccctgaag gtagctagcc cgccgcggcg
      241 cgtttttcga cgcgctcgga gagggcactg gctacgtgtt gcaacaactc ttggtcggta
      301 tacggtttgg tgagatagac attcacgccg ctggccatcg cctggcggcg atgtttgtcc
      361 agcgaacgcg aggtgatcat gatgaccggc aggttcgaaa gctcgggtcg cgcgcgaacg
      421 tgcgcggtga gttcgaggcc gttcatgttg ggcatttcga gatcggtgag cagcgcgtcg
      481 ggacgggccg cttccatcgc cttcacggcc tcgaggccgt cgcgcgcgag cgcgacgtcg
      541 tagccgtggt cttcgagcag cgtggcgacg gcgcggcgca ccgacatgga atcgtcgacg
      601 atgagtacgc gcgagcggcg cagttccttc gcctgtgaac gcgcttcggc cgcggcgcgc
      661 atcgcctggt cgcgcggcgt gcgcgcgagc tcggcgatgt cgagcagcgg gatcacggcg
      721 ccgttgccgg tgaggccgat gcccgtgacg ccgggaatac gtttgagata acggccggtg
      781 gggcgcgtca cgagttcgct ggcttcgagc acggcgtcga cgagcacgcc gatgggcccg
      841 gattcgctgt ccacgagcac gagattgcga cgctcgggcg cggtcgcggg gcgcatgccg
      901 gtgagcatcg cgagttcatg gaccggataa aggatgtcat cgacgcgcgc gaacgccacg
      961 ccgtcctcgc gttcgatctt cacggccacc gccggtacgg cggtgcgcac ggcgtggctc
     1021 gcgataccga agatctggcc gccatcgcgc acgaacaacg cgtgcgtggc gacgagtgaa
     1081 gcctggaaac gcagttcgat cgagaggccc gcgcccgctt cggagcggat gtcgatggat
     1141 cccgagagcg cacgcagacg cgtggcgacc acgtcgaggc cgacgccgcg gcccgagatt
     1201 tcggtcaccg cgtcgcgcgt ggagaagccg ggcagcaagg tcaggcgcgc gatttcggcg
     1261 tcgctggggg tggcgtccac ctccagcagt ccacgctcga tggccttggc gcgaatgtcg
     1321 gcgaggttga gaccgcggcc gtcatcggcc acctgaacgg tcacgacctg gccacggcgc
     1381 gccacggtca gcgtgatcgt gccggcgcgc ggcttgccca ccgactcgcg ttccgccggt
     1441 gtttccagcc catggtcgac ggcattgcgc aggatgtgca gcaacggatc gatcaactga
     1501 ttcagcactt cggcgtcgac gaggatttcc gagccgacga ccacgagcga cgcctgcttg
     1561 taagtcgcgg cagcagtctg gcgcacgttg cgctgcagac gcggcacgat ggtctcgacg
     1621 ggagccatgc gtgtctgcga ggtgagatgt tgcagttcgc gcatgagtcg ctgctgctgg
     1681 tgcaggatgc ccgccaggcc tgcgagttcg tccttgagtc cgagccccag gtcgcgcgca
     1741 tcggtggttt cttcgatgag cgcgcgcgtg agcgactgca cttcgttgta ctggtccatt
     1801 tccagcgaat cgaagtccga tgcggcgtgc acgacattca gtttgtggcg cgcggcaacg
     1861 ccgcggatgt cgaccgaatg ttcgagctcg taaagccgct tctggatcgc gcgatcgcgc
     1921 tcgatcaatt cacgggaatg gccggacgcg gacttcaatt cctggtcgag ttcgccgatc
     1981 ttgatcgtga gctcgcccac catgcgcacc aggttgtcga gcaactcggt gggcacgcgg
     2041 gtggtggcct tggcttcggt gcgcggtgcg gccgcggccg caggcgcggc cggtgcggcc
     2101 tgtacgggca cgggcatttc gagcgcacgg tggcgcacgg tgtccgaggc gtcgttgttg
     2161 cgcgcggcat cgagtagttc gatgacgccg agcgcattct ccggggccga gtcttcaccc
     2221 gccacggtgg ccaccatctg ctcgaggcag tcggcggcgg atacgagcgc ctgcgcgcgc
     2281 atggaagagg gcggcgcgct ttcggcttcg cagatttcca ggatgtcttc gaggcggtgc
     2341 gccagcatgg cgacgccgcg gatgcccagg atgttcgccg agcccttgag agtgtgcgcg
     2401 gcgcgcttgg cattgcgcag cagctcggat tgcgacggat cggcgatcca tccctgcagc
     2461 gattgcgtga cttcggcggc ctgcggcggc gagtcgtgaa ggaatgcgtc gatcagcgaa
     2521 gcatcgacgt cgctagaaat ggcgagggag agatccgcag cgtcgggcgc ttcgggcgcc
     2581 gcctgttcgc tctcgtcgag cgcggcgccg atggagttgg ccgcggcgag ctcggcgcgc
     2641 aggtcgttca gcgcggactc atcgagcggc aacggcaccc agccaccgcc gaaatgcgcg
     2701 atcagcgagc cgatcggtgc cggcgagttc gcgtcgcgca gatgcgcttc gagcagcctc
     2761 ggccagtcgg tgaagaacgg ccgcaccagc acgcgggcat cctgatcgtc ggcgtcgagg
     2821 tgctgaagat tgtcgagcac gcaggcggcg atgcgctgca cgcccgcgag ccccatgtag
     2881 cctgcggctt cagaaagtcg ggccacggac gttacatagg catcgtcacc cggagaaccc
     2941 gagtcgtatg cggccgcgag ctcgggtgcg agacccttga gctcgtcggc aagcgctgtg
     3001 aggagatcct gtcctcgcat actggcgttc cgtgccttcc cttatgccgc gcgaatggtg
     3061 ggtagtttga acacgcgcac cgattcgacg agtcggtcgg cggacttcga caggtcggcg
     3121 gtggcctcgg actgaagcag aatctccgcc gcggtctttt ccgtgctggc gccgatctgg
     3181 tgcacccgat tgcgcagctc ggtggtgatc ttcatctgca cctgggccga cgaggtgatg
     3241 cgtttcacga gctccacgag cgacgcggtg gactgctggg tttcgcgcat ctgcgcgccc
     3301 gagcgtgccg cgatctcgga acccgacacc acctgcgcga tggtcttgtt gaccgtgttg
     3361 atcgtgtccg acgtttcgat ctgaatgttg ttcacgagct gcgcgatctg ctgcgtcgcc
     3421 tggcgtgagc tctcggcgag gcgctgcact tcttcggcca cgaccgcgaa tccgcggccg
     3481 gcgtcaccgg cggcggcggc ctgcatggat gcgttcaacg ccagaacgtg cgtacgttcg
     3541 gcaatcgtgt tgatgaggtt cacgatgccg gtgatttcct gcgaacgttc gcccaggcgc
     3601 ttgatgcgct tttcggactc ggcgatcgat tcgcgaatgc cttccatg
//
LOCUS       DATDEU010000051         4480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_446402, whole genome shotgun sequence.
ACCESSION   DATDEU010000051 DATDEU010000000
VERSION     DATDEU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446402"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..2165
                     /locus_tag="VIV63_01090"
     CDS             123..2165
                     /locus_tag="VIV63_01090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEY6123216.1"
                     /translation="MTLDLTQFHEIFFEESFEALDQMEAALLKLSAGEVDADLINTIF
                     RVAHSIKGGAATFAFNEVAGFTHVLETLLDQLRAGKRQVRPDIVDVLLRSGDAMRAML
                     AATQHKQAIDTAAVATLRAELDRIMSESTTAAAPAAVATAAKAAAPDAPPPLPGWRIK
                     FIPGPDLLRHGNDPLRILRELATLGNLKVSVDAAGIPPVAELDPEVCRLRWNLELQGE
                     IDRDAVRAVFEWAETECELTIERIGGELIDDVAPAAVAAPVADSSGTAAPAKAESSAR
                     TASPDGGSIRVSIEKIDELLNSVGELVITQSVLSQLAAPLEGREAGELRSALAQLERH
                     MRALQESVMRVRMLPISFVFNRFPRLVRDLGQRLGKKIELKVTGDTTELDKTVLEKIG
                     DPLVHLVRNSIDHGIEMPDVRLAAGKTEHGTISLHAYHKGGNVIVEVSDDGGGLRKDK
                     ILKKALERGLVTNDVELSEERILNLIFAPGFSTADVVSDVSGRGVGMDVVRRNINELG
                     GHIQIFSTQGQGSTIRIRLPLTLAILDGQLARVGKEIYVVPIVSIVETIQVTRERVCA
                     VASGGQVFRLRDDYLPIVRLHSLFDIEPDYTDLLDGLLMIVEADGKRVGLFVDELMSQ
                     QQVVIKSLETNFKQITGLAGATMLGDGRVALILDIPGVITRFQAPVDRGMAKPQAA"
     gene            2243..>4480
                     /locus_tag="VIV63_01095"
     CDS             2243..>4480
                     /locus_tag="VIV63_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6123217.1"
                     /translation="MSFSDFKVGTRLSVGFAILLAITIAVGGVGLMRLSQLDRMVTQI
                     TDVDWEKARISMEMEARNRENAAKFSRLLLVDGESEKAAALKAKIASNSTKNAEAIKE
                     LETLLASPEEKALLAEAAAARETYNASRAEVMKLVADPKTRAAGLEQYNTVTADLIEP
                     YVEALNKLTVMQQEIFEKASEKSAAAYDHSRNLILAIIGGAVFFGIVLAVMLARSIVR
                     PLGNAMAIAESIKEGRLDNAIDTTGSDETAKLLGSLDHMQSALRARDEKDADFRGQIS
                     AIGRSQAVIEFDMNGTVRDVNDNFARVMGYSRAEVIGKHHSMLVESGYSASPEYRSFW
                     EKLNRGEPDINSYKRIAKGGREVWIQASYNPIPDLSGKPFKVVKYATDITEQKVRAAD
                     FEGQLTAISRSQAVVEFELDGTIRSVNDNFAQVLGYSNSEVRGKHHSMFVDPAISGSA
                     EYRAFWAKLGRGEADAGRYRRVGKGGREVWVQASYNPILDANGRPFKVVKYATDVTQQ
                     MQMAHQLELTVKQTQITVQAATEGDLTARIPLTGKTGELEALCRGVNSMLDSTADLVK
                     RVKTASAEVQAGAEEISKGNLNLSQRTEEQASSLEETASSMEEMTSTVKQTADNAGQA
                     NQLAMAARQQAEKGGAVVSSAVTAMGGINAASKKIADIIGVIDEIAFQTNLLALNAAV
                     EAARAGEQGRGFAVVATEVRNLAGRSATAAKEIKSLIQDSVARVDAGSKLVDESGRTL
                     EDIVNA"
BASE COUNT          913 a         1451 c         1418 g          698 t
ORIGIN      
        1 atcgtcgcct tcgcgctcga ctgcctcgag aagggcgtcg cctacaagtg gaccggacgt
       61 tcggccaagc tggacgccgc catcgaacta ctcggcgtga gcccgttgct ggaatacccg
      121 gcatgacgct cgatctcacg caattccacg aaatattctt cgaagagagt ttcgaagcgc
      181 tcgaccagat ggaagcggcg ctgttgaagc tgtcggccgg tgaagtcgac gccgatctca
      241 tcaacaccat cttccgcgtg gcgcattcca tcaagggcgg cgccgcgacc ttcgcgttca
      301 acgaagtggc cggctttacg cacgtgctcg agacgctgct cgaccagctg cgcgccggca
      361 aacgccaggt tcgtcccgac atcgtcgacg tgctgctgcg ttctggcgac gccatgcgcg
      421 ccatgttggc ggccacgcag cacaagcagg ccatcgacac cgctgcggtt gccacgttgc
      481 gcgccgagct cgaccgcatc atgagcgaga gcacgacggc cgcagcgccc gcggccgttg
      541 ccacggccgc caaagccgca gccccggacg caccgccacc gttgcccggc tggcgcatca
      601 agttcatccc gggtccggat ctgctgcgtc atggcaacga cccgctgcgc atccttcgtg
      661 agctcgccac gctcgggaat ctgaaagtct cggtggacgc cgcgggcatt ccgccggtcg
      721 ccgaactcga tccggaagtc tgccgcctgc gctggaatct cgaactgcag ggcgagatcg
      781 atcgggacgc cgtgcgtgcc gtattcgaat gggcggaaac cgaatgtgag ctcaccatcg
      841 aacgcatcgg tggcgaactg atcgacgatg tcgcaccggc cgccgtggcc gcgccggtcg
      901 ccgacagcag cggcaccgcc gcgcctgcga aagccgagag ctcggcccga accgcgagcc
      961 ccgacggcgg gtcgattcgc gtcagcatcg agaagatcga cgagttgctg aattccgtcg
     1021 gcgaactcgt gatcacgcag tccgtgctga gccagctcgc cgcgccgctc gaaggccgcg
     1081 aggccggtga actccgctcc gcgctcgcgc agctggagcg tcacatgcgc gcgctgcagg
     1141 aaagcgtcat gcgcgtgcgc atgttgccca tcagtttcgt gttcaaccgc ttcccgcgcc
     1201 tggtgcgcga tctcggccag cgtctcggca agaagatcga actcaaagtt accggcgaca
     1261 cgaccgagct cgacaagacc gtgctcgaga agataggcga cccgctggtc cacctggtgc
     1321 gcaactcgat cgaccacggc atcgagatgc ccgacgtccg cctggccgcc ggcaagaccg
     1381 aacacggcac catcagcctg cacgcctatc acaagggcgg caacgtgatc gtcgaagtga
     1441 gcgacgacgg cggtggcctg cgcaaggaca agattctcaa gaaggcgctc gagcgcggtc
     1501 tcgtcacgaa cgacgtcgag ctgtccgagg agcgcatcct caatctcatc ttcgcgccgg
     1561 gcttttccac ggccgacgtc gtcagcgacg tgtcgggacg cggtgtgggc atggacgtgg
     1621 tccgccgcaa catcaatgaa ctcggcggcc acatccagat cttctcgacg caggggcagg
     1681 gcagcaccat ccgcatccgc ctgccgctca cgctcgccat cctcgatggc cagctcgcgc
     1741 gcgtcggcaa ggaaatctac gtggtgccga tcgtctcgat cgtcgagacc atccaggtca
     1801 cgcgcgagcg cgtctgcgcg gtggcgagcg gcggccaggt cttccggctg cgcgacgact
     1861 acctgcccat cgtgcggctg cacagcctgt tcgacatcga gcctgactac accgacctgc
     1921 tcgacgggtt gctcatgatc gtcgaggccg acggcaaacg cgtgggcctg ttcgtcgatg
     1981 aactgatgtc ccagcagcag gtggtcatca agagcctcga aaccaacttc aagcagatca
     2041 cgggactcgc gggcgccacc atgctcggtg acggacgcgt ggcgttgatc ctcgacattc
     2101 ccggcgtgat cacccgcttc caggcgcccg ttgaccgcgg catggcgaag ccgcaagccg
     2161 catgagcgct tcttcgatgc acaccggaaa cgccagcgga gcgcctggtc gacagagcaa
     2221 ttcactgatt acgaggatgt aaatgagctt ctccgatttc aaagtgggca cgagattgag
     2281 cgttggcttc gccatcctgc tggcgatcac gatcgccgtc ggcggcgtgg gcctgatgcg
     2341 tttgtcgcag ctcgaccgga tggtcacgca gatcacggac gtggattggg agaaggcgcg
     2401 gatcagcatg gagatggagg cacgcaatcg cgaaaacgcc gcgaaattct cccgcctgct
     2461 gctggtcgac ggcgagagcg agaaagccgc cgccctgaag gccaagatcg cctcgaacag
     2521 cacgaagaac gccgaagcca tcaaggagct cgagacactt cttgcatcgc cagaagagaa
     2581 agcgttgctg gcggaagccg ccgcggctcg cgaaacctac aacgcatcgc gggcggaagt
     2641 catgaagctc gtggcggatc cgaagacgcg cgcggccgga ctcgagcagt acaacaccgt
     2701 gactgccgac ctcatcgagc cgtacgtcga ggcgctgaac aagctcaccg tgatgcagca
     2761 ggaaatcttc gagaaggcca gcgagaaaag tgccgcggcc tacgatcact cccgcaatct
     2821 cattctggcg atcatcggcg gcgccgtgtt cttcggcatc gtgctcgcgg tgatgctggc
     2881 acgaagcatc gtccggccgc tgggcaatgc catggcgatc gccgagtcca tcaaggaagg
     2941 tcgactcgac aacgcgatcg acaccacagg cagcgacgag accgcgaaac tgctcgggtc
     3001 actcgatcac atgcagagcg ccctgcgcgc ccgcgacgaa aaggacgcgg atttccgcgg
     3061 ccagatttcg gccatcggcc gatcacaggc cgtcatcgag ttcgacatga acggcacggt
     3121 acgcgacgtg aacgacaact tcgcgcgcgt catgggttac tcccgcgccg aagtcatcgg
     3181 caagcatcac agcatgctcg tcgaaagcgg ttattcggcg agccccgagt atcgttcctt
     3241 ctgggaaaag ctcaatcgcg gcgaaccgga catcaacagc tacaagcgca tcgcgaaggg
     3301 cggccgcgaa gtgtggatcc aggcgtcgta caacccgatt ccggatctct ccggaaaacc
     3361 attcaaggtc gtcaagtacg cgaccgacat caccgagcag aaggtgcgcg ccgccgattt
     3421 cgaaggccag ctcacggcca tcagccgctc gcaggcggtc gtcgaattcg aactcgatgg
     3481 cacgatccgc tcggtcaacg acaactttgc gcaggttctc ggttactcca acagcgaagt
     3541 gcgcggcaaa catcacagca tgttcgtcga cccggcgatc agcggcagcg cggaataccg
     3601 tgcgttctgg gcgaagctcg gacgaggtga agcggacgcg ggtcgttatc gccgcgtggg
     3661 caagggtggc cgcgaagtct gggtgcaggc ttcctacaac ccgatcctcg atgccaacgg
     3721 ccgtccgttc aaagtggtca agtacgccac ggacgtgacg cagcagatgc agatggcgca
     3781 ccagctcgag ctcacggtga agcagacgca gatcaccgtg caggccgcga ccgaaggcga
     3841 tctgacggcg cgtatcccgt tgacgggcaa gaccggagaa ctcgaggcgt tgtgccgtgg
     3901 cgtgaattcc atgctcgatt cgactgccga cctggtcaag cgcgtgaaga cggcttcggc
     3961 tgaagtgcag gccggcgcgg aagagatttc caaaggcaac ctgaatctct cgcagcgcac
     4021 cgaagaacag gcgtcgagtc tcgaagagac cgcgtcgtcc atggaagaga tgacctcgac
     4081 ggtcaagcag accgccgaca acgccggcca ggccaaccag ctcgcgatgg cggcacgcca
     4141 gcaggcggag aagggtggcg cggtagtcag ctcggccgtc acggccatgg gtggcatcaa
     4201 cgcggccagc aagaaaattg ccgacatcat cggcgtcatc gacgagatcg cgttccagac
     4261 gaacctgctg gcgctcaacg ccgcggtgga agccgcgcgc gccggcgaac agggccgcgg
     4321 cttcgccgtg gtggccacgg aagtccgcaa cctcgcggga cgctcggcga cggcggccaa
     4381 agagatcaag tcgctcatcc aggacagcgt cgcgcgcgtg gatgcgggca gcaaactcgt
     4441 cgatgaatcc ggccgcacgc tcgaagacat cgtgaacgcg
//
LOCUS       DATDEU010000052         8265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1467638, whole genome shotgun sequence.
ACCESSION   DATDEU010000052 DATDEU010000000
VERSION     DATDEU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467638"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VIV63_01100"
     CDS             complement(<1..223)
                     /locus_tag="VIV63_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123218.1"
                     /translation="MTDNVLKVRPAAETRWDCASFGEIMLRFDPGFGRVRNARTFQVW
                     EGGGEYNVARAMRKCWGKRSTAITALPVND"
     gene            complement(245..916)
                     /locus_tag="VIV63_01105"
     CDS             complement(245..916)
                     /locus_tag="VIV63_01105"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY6123219.1"
                     /translation="MNKDRMSVLAAMMDQGVIPVFYHPDVEVCKKVIQACANGGAKCI
                     EFTNRGDFAAHVFYDVARHFDKADPSVIMGVGSIVDAPTAGIYIANGAKFVVGPILNA
                     DVAKVCNRRAIPYSPGCGSASEISYAQELGSEIVKVFPGSSVGGPEFVKNVMGPMPWT
                     RIMPTGGVEPTEESLRKWFGAGIVACGIGSNLITKELLAKQDYAGVEARVRDTVKLIK
                     TIRQK"
     gene            complement(916..1122)
                     /locus_tag="VIV63_01110"
     CDS             complement(916..1122)
                     /locus_tag="VIV63_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123220.1"
                     /translation="MQKTGDKMTPVTLALVIDDSKLERKTWAGYGKTESLCPCFGVAT
                     LEQLFAAAVAGQRKCRRFTNEWRD"
     gene            1158..4511
                     /locus_tag="VIV63_01115"
     CDS             1158..4511
                     /locus_tag="VIV63_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123221.1"
                     /translation="MAISNRTKGILPVVLGSLGVATGAIAADQDQGAAASEAQNGSAS
                     SAAEDIGEIEVTGYRLSLRNAIDVKKNASVMMDAINAEDIADFPDANLAESLQRLPGI
                     ALDRDNGEGRSITVRGLGSDFTRVRLNGLETLSTAAASDSGTSPNRGRGFDFNVFASD
                     LFTSLEVRKTASASTDEGSLGATVDLETGKPLDFKGRRVALSLEDAYYENGGTHNPRV
                     AALFADQWFDDTFGVAFSVAYSERDSEVDRFKRQAGQSDYEYRGSTWAGNETPARAGF
                     AAPAGTTFGTIITNPNAIAALTGSDAAAYAAMHPAAYSTNLTNSLVRVPALMNIEQQD
                     LAQERLGVTAALQWQPTESTLISLDTVYSKFDQKSDVNQIQSVGLNRNNTNANFNTAT
                     TPTSLSVANRRGTYQICTPQTAEAFRDPIDCGGSEAMPGGVFAGLGTTSFSTNPNNLE
                     PYDYYNNPGSVGYGGAANVAAANGLYFRDRFLGRPGVDVLAAHVSDAGNADYLELRNV
                     DWRSATDSSYFTTEFKQASLNIQQDIGDQLKVDILYGKSESLNDNQGLLVEFNRMDSP
                     ETFIYDERAHGSMPAISYGFDLADANNWSLVKGFSTMRHFQRETDNEYEGGHINFNLK
                     MTDHLNLEFGGTKREYKFSTNQGQRLSNENINPTLAELGVAATDLGRVYDFGDGLDLP
                     AGSPTAFFAPNIDAFREITGFDCNCLNEYGDFRLSYLSNPGNQFGVEEYDTSYFVQLN
                     WDFELFSRRMFGNYGIRSADTRLVSTGYTTNVAATGPRPLEARHSYSDNLPSLNLGYM
                     LTDTLMLRAGYAKVMARPQLQNLSPSITGLTTPTVAGTIGALTIGNPYLSPFRATNYD
                     LSLEWYFSEGGLLSFAVFKKDVSNFPQTVSSAGDLSTVMTPDQIAAFLQTQTPQQIAW
                     ILAGNGGNPGIYSIRQFEDAPGGEIEGYEISYQQDLNFEFLPAWARNFGVQANFTKLD
                     SELQYILDPGSTTAPVRPQVTAGGPFTGASPESANFTLYYETPKWSARASWAYRSAYV
                     SQYPIAAGTCDPGVCDAPLINDFLGSRATRNIDASVRYQFNDHLSMSIEGLNLTNQTE
                     DRWAYEAEPLVTQYSSTGRQIFAGFRLSL"
     gene            4785..7424
                     /locus_tag="VIV63_01120"
     CDS             4785..7424
                     /locus_tag="VIV63_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6123222.1"
                     /translation="MVAIILLVFYGWSQRGWLDNVADIESRDVDMQSQQLQRGFATEL
                     DDLYAAMGDYAIWNETAEFARGMRPDFLAESLNAAALVRLDVDTFLVLDRNLDTRASL
                     ALDAAAAQEYEIPPDPELLAVIKQAVTSGQLNSNNDSVRGIVQRARGPALFAARPIFD
                     STGGGSPQGWIAFARAFTPTVVARMGRFSPWPVTGFPVANLDRAGVPEEVRDWVHHSP
                     LTASVLTRVAGRNHLTGYLVLRDQLGTPVWLIALEIPRSAYAQAKRTTLYQTIFLGLL
                     VTGFTIVALLLVARSRRLNADRLSLESRYRAIIEQAQDGLLVADARTGEIVDANPAVQ
                     QQLGFTLDELRGRSVLSVLRGPNDSQDPVIATLARVEPSRGIELVQWLKDGRQMDVEV
                     SCVPIESRDRRLVSYLMRDLSERKKAQTQLLANQQRLDKLANHDHLTGLPNRLFLQAH
                     LPEAIGRCQESGQMLAVLFLDLDRFKHINDSRGHEVGDKLLQEIAKRVRAAVRPADIV
                     VRMGGDEFVVVLHKVNSPDEVGIAATRINEVLSAPVMIDGRALVATVSIGVSLFPRDG
                     ATMGELLKHSDTAMYQAKDLGRNNFQVFSPQMDRALKERVTTESSLRVALKLNQFDVH
                     YQPIIDIHSRRVAGLEALLRWKHPTLGFVPPDRFIEVAEETGLIIPIGQFVLDRIGTD
                     IAKWREQGAHLVPVSMNVSAVQLERTKLRELIQQAMLKNRIGPQLIQLELTEGSLFET
                     RTGEFREDALATLRDLGVKIAIDDFGTGYSSLSYLKRWRVDSLKIDRSFVRDIATDPS
                     DHAIVSAIVAMARSLNIQVVAEGIETWQQLEILRNMGCSLAQGFLFAKPCPASDALRY
                     LRVEPLDLLESNWESSNSLAETG"
     gene            7480..>8265
                     /locus_tag="VIV63_01125"
     CDS             7480..>8265
                     /locus_tag="VIV63_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HEY6123223.1"
                     /translation="MKGWTGKAAFAAAFVITSTIVLAADTDDSLGREQLHATLWMQTA
                     PEYRAISLQVYRQAEERIAAPLPGSAALEQAAVPAETLARLPTAVVLDLDETVLDNTV
                     YQARLLRDRATYNTTTWGEWVAAGEAEALPGSREFIARARALGHTVFYISNRDCTAPP
                     PTAADPCPAKTATMKNLVALGIEDAPDPKRMLLRGERPEWNTGNKTQRRAFIAANYRI
                     VALVGDDLGDFTDPQVFAGDRERLEPRFGATWFLLPNPIYGSWT"
BASE COUNT         1571 a         2698 c         2507 g         1489 t
ORIGIN      
        1 gatcgttgac gggcagggcg gtgatcgccg tcgaacgttt gccccagcac ttgcgcatgg
       61 cgcgcgcgac gttgtattcg ccgccgcctt cccagacctg gaacgtgcgc gcattacgca
      121 cgcggccgaa gccgggatcg aagcgcagca tgatttcgcc gaacgaggcg cagtcccaac
      181 gcgtttccgc ggccggtcta actttcaata cgttatcagt catgacttcc tgtttctctt
      241 gctcttactt ttggcgaatg gtcttgatga gtttcacggt gtcgcgcacg cgcgcttcga
      301 cgccggcgta gtcctgtttc gcgagcagtt ccttggtgat gaggttgctg ccgatgccgc
      361 aggcgacgat gccggcgcca aaccacttgc gcaatgattc ttcggtcggc tcgacgccac
      421 cggtcggcat gatgcgcgtc cacggcatcg ggcccatgac gttcttcacg aactccggtc
      481 cgccgaccga actacccggg aacaccttga cgatttcgct gccgagctcc tgcgcgtagg
      541 agatctcgct ggccgagccg cagcccgggc tgtagggaat cgcacggcga ttgcagacct
      601 tggcgacatc ggcgttgagg atcgggccga cgacgaactt cgcgccgttg gcgatgtaga
      661 tgccggcggt gggagcatcg acgatggagc cgacacccat gatgacgctt gggtccgcct
      721 tgtcgaagtg acgcgccacg tcgtagaaga cgtgcgccgc gaaatcgccg cgattggtga
      781 actcgatgca cttcgcgccg ccgttcgcgc aggcctggat gactttctta cagacctcga
      841 catccgggtg atagaaaacc gggatgactc cctggtccat catcgcggcc agcacgctca
      901 tacgatcctt attcatcaat ctctccattc attagtgaag cgccggcatt ttcgttgacc
      961 ggcgacggcg gcggcaaaca attgttcaag agtcgcaacg ccgaagcagg gacacaagct
     1021 ctctgttttg ccatatcccg cccatgtctt gcgctcgagt ttggagtcgt ctataacgag
     1081 cgccaaggta accggtgtca ttttgtcgcc ggtcttctgc aaacaacaag tatcgaatcg
     1141 aagagggagg gggaaacatg gcgatctcaa atcgcaccaa gggaattttg cctgtggtgc
     1201 tcggtagttt aggcgtggcg accggcgcga tcgcggccga tcaggaccag ggggcggctg
     1261 cttcggaagc gcagaacggc agcgcttcga gcgccgcgga ggatatcggc gagatcgagg
     1321 taaccggtta ccgcctgtcg cttcgcaatg cgatcgacgt caagaaaaat gcctccgtaa
     1381 tgatggatgc catcaatgcc gaggacatcg ccgacttccc ggacgccaac ctggcggaat
     1441 ccctgcagcg cctgccagga atcgcgctcg accgcgataa cggcgaaggc cgcagcatca
     1501 cggtgcgtgg tctgggcagc gacttcacgc gtgttcgcct gaacggcctc gaaacgcttt
     1561 ccactgccgc cgccagcgat tccggcacct cgccgaaccg cggccgtggt ttcgacttca
     1621 acgtgtttgc ttcggacctc ttcacctcac tcgaagtgcg caagacagcc tccgcgtcga
     1681 cggacgaagg ttcgctcggc gccactgtcg atctggaaac gggcaaaccg ctcgacttca
     1741 agggccgccg cgtcgcgctc tcgctggaag acgcgtatta cgaaaacggt gggacgcaca
     1801 acccgcgcgt tgccgccctg ttcgcagacc agtggttcga cgacacattc ggcgtcgcgt
     1861 tttcggtcgc gtacagcgag cgcgattccg aggtagaccg cttcaagcgc caggccggcc
     1921 aatccgatta cgaatatcgc ggctcgacct gggcgggcaa cgagacgccg gcgcgcgccg
     1981 gtttcgcggc gccggcgggt acgaccttcg gcacgatcat caccaacccg aacgccatcg
     2041 cggcactcac cggatccgat gcggccgcct atgccgccat gcatccggct gcgtacagca
     2101 ccaacctcac gaactcgctg gtgcgcgttc cggcgctcat gaacatcgaa cagcaggacc
     2161 tggcgcagga gcgcctcggc gtgactgctg cgctgcaatg gcagccgacg gaatcgacgc
     2221 tgatcagcct ggataccgtc tattccaagt tcgatcagaa gagcgacgtc aaccagattc
     2281 agtccgtagg actgaatcgc aacaacacca acgcgaactt caacaccgcg accacgccca
     2341 cttcgctgtc ggtggcgaat cgccgcggca cttaccagat ctgcacgcca cagaccgcgg
     2401 aggctttccg cgatcccatc gattgcggcg gttcggaagc gatgcctggc ggtgtgttcg
     2461 cgggcctcgg cacgaccagc ttcagcacca accctaacaa cctcgaaccg tacgactact
     2521 acaacaatcc gggctcggtg ggatatggcg gcgctgcgaa cgtcgcggcg gccaatggcc
     2581 tgtatttccg cgatcgtttc ctgggccgcc cgggtgtcga tgtgcttgcc gcgcatgtct
     2641 ccgatgccgg caatgccgac tacctcgagc tgcgcaacgt ggactggcgc tcggcgaccg
     2701 actcgtcgta cttcacgacg gagttcaagc aggcgtcgct caacatccag caggacatcg
     2761 gcgaccagct caaggtcgac atcctctacg gcaagtccga gtcgctgaat gacaaccagg
     2821 gactgctggt tgagttcaat cgcatggatt cgcccgagac tttcatctac gacgaacgcg
     2881 cccatggttc gatgccagcg atcagctacg gcttcgatct cgccgacgcg aacaactgga
     2941 gcctggtgaa agggttctcg acgatgcgcc atttccagcg cgagacggac aacgagtacg
     3001 aaggtggcca catcaacttc aacctgaaga tgaccgacca cctgaacctg gaattcggcg
     3061 gcacgaagcg cgaatacaag ttctccacca accagggtca gcgcctgtcg aatgaaaaca
     3121 tcaatccgac gctcgccgaa ctcggcgtcg ccgcgacgga tctcggccgg gtgtatgact
     3181 tcggcgatgg actcgatctg ccggcgggtt cgccgacggc gttcttcgct cccaacatcg
     3241 atgccttccg cgagatcacc ggcttcgatt gcaactgcct gaacgagtac ggcgacttcc
     3301 gcctgtcgta cctgtcgaac ccgggcaacc agtttggcgt cgaggaatac gacacgagct
     3361 acttcgtgca gctcaactgg gacttcgaac tgttcagccg ccgcatgttc ggtaactacg
     3421 gcatccgctc cgccgacacg cggctggttt ccacgggtta cacgaccaac gtcgccgcga
     3481 cgggaccgcg gccgctcgaa gcgcggcatt cgtacagcga caacctgccg tccctgaacc
     3541 tcggttacat gctcaccgac acgctgatgt tgcgcgccgg ctatgcgaaa gtgatggcgc
     3601 gcccgcagct ccaaaacctc tcgcccagca tcaccggact caccacaccg acagtggcag
     3661 gcacgattgg agcgttgact ataggcaatc cgtatctctc gccgttccgt gcgaccaact
     3721 acgacctgag cctcgagtgg tatttcagcg aaggtggcct gctgtcgttc gcggtgttca
     3781 aaaaggacgt gtccaacttc ccgcagacgg tgtcgagcgc tggcgatctc agtaccgtca
     3841 tgacaccgga tcagatcgcc gcgttcctgc agacccagac cccgcagcag atcgcgtgga
     3901 tcctcgcggg taacggtggc aacccgggca tctactcgat ccgccagttc gaagacgcac
     3961 ctggcggcga gatcgagggt tacgagatca gctaccagca agatctcaac ttcgaattcc
     4021 tgccggcgtg ggcgaggaac ttcggtgtgc aggccaactt cacgaagctg gattccgaac
     4081 tgcagtacat cctggatccg ggttcgacca ccgctcccgt gcggccgcag gtcactgccg
     4141 gtggtccctt cacgggtgcg tcaccggagt cggcgaactt cacgctgtac tacgagactc
     4201 cgaagtggag cgcgcgcgct tcctgggcct accgctcggc gtacgtgagc caatacccga
     4261 tcgcggccgg tacctgcgat ccgggcgtct gcgatgcacc gttgatcaac gacttcctgg
     4321 gcagccgggc aactcgcaac atcgatgctt cggtgcgcta ccagttcaac gatcatcttt
     4381 cgatgtcgat cgaaggcctg aatctcacca accagactga agaccgctgg gcgtacgagg
     4441 ctgagccgct ggtcacccag tactcgagca ctggccggca gatcttcgcc ggtttccgcc
     4501 tgagcctgta aacctcggtc gaacgtcggc ccacgaagcg ccggtaccct gaagggtgcc
     4561 ggcgctttgt cgtttccggg taccgctcga gcccgtgtcc tacgcgctga cgcccagtat
     4621 ttaagaggaa aatgtgcgcg gttcgatgcc gtcgatcgtg gtgcgccagt caaactctgg
     4681 agtggccaga aatcgcacgt gacgcagttc acgttatgaa tcgcaccagc aattctgaac
     4741 cgaaaggaag gatcagacag tctccgctgc ccgtaatcgg cgcggtggtc gccatcattt
     4801 tgctggtgtt ttacggctgg tcgcagcgcg gctggctgga caatgtggcc gacatcgaat
     4861 cgcgtgacgt cgacatgcag agccagcagc tgcaacgagg attcgctacc gagctcgacg
     4921 acctctacgc ggccatgggc gactacgcca tctggaacga gaccgcggaa ttcgcccgag
     4981 gcatgcggcc ggacttcctg gccgagtcgc tcaatgccgc cgcgctggta cggctcgacg
     5041 tcgacacttt cctcgtgctc gaccgcaacc tcgacacgcg cgcgtcgctc gcgctcgatg
     5101 cggccgccgc gcaggaatac gagataccgc ccgatcccga attgctggcg gtcatcaagc
     5161 aagccgtcac gtcaggccag ctgaacagca ataacgacag cgtgcggggc atcgtccagc
     5221 gcgcgcgagg accggcgctg ttcgccgcgc gtccgatctt cgacagcacg ggcgggggat
     5281 cgccgcaggg ctggatcgct ttcgcacgcg cgttcacgcc gaccgtggtg gcgcgcatgg
     5341 gccgcttctc gccgtggccg gtcaccggct ttcccgttgc gaacctggat cgcgccggcg
     5401 tgccggagga ggtgcgcgac tgggtacatc attcgccgct gaccgcgagc gtgctgacgc
     5461 gagtggccgg gcgtaatcac ctcaccggat atctcgtact gcgcgatcag ctcggaactc
     5521 ctgtctggct gatcgcgctc gagattccgc gcagcgcgta tgcgcaggcg aagcggacga
     5581 cgctctacca gaccatcttc ctcggccttc tcgtcaccgg cttcaccatc gtcgcgttgc
     5641 tgctggtggc gcgatcacgg cgcctcaacg cggaccgctt gtcgctcgag tcgcgctacc
     5701 gggcgatcat cgaacaggcc caggacggac tgctggtggc ggacgctcgc accggcgaga
     5761 tcgtcgacgc caacccggcc gtgcagcagc agctgggatt cacgctggac gaattgcgcg
     5821 ggcgctcggt gttgtccgta ttacgcggcc cgaacgattc gcaggacccg gtcatcgcga
     5881 cgctggcgcg cgtggagccg agccggggca tcgagctcgt gcagtggctc aaggacggcc
     5941 gccagatgga cgtagaagtg agctgcgttc ccatcgagtc tcgcgatcgc cggctggtgt
     6001 cgtacctgat gcgcgacctg tccgagcgaa agaaggcgca gacgcagctg ctcgccaatc
     6061 agcagcgcct cgacaaactc gccaatcacg atcacctgac cgggttgccg aaccgcctgt
     6121 tcctgcaggc gcatctgccc gaggccatcg gacgctgcca ggaatcggga cagatgctcg
     6181 cggtgctgtt cctcgatctc gaccgcttca agcacatcaa cgattcgcgc ggccacgaag
     6241 tgggcgacaa actactgcag gaaatcgcca agcgcgtgag ggcggcggtg cgtcccgccg
     6301 atatcgtcgt gcgcatggga ggcgacgagt tcgtcgtcgt gctccacaag gtcaattcgc
     6361 ccgatgaagt cggcattgcc gcgacgcgta tcaacgaggt gttgagcgcg ccggtcatga
     6421 tcgacggcag agcactggtc gccaccgtca gcattggcgt gagcctcttc ccgcgagatg
     6481 gcgccaccat gggcgagctg ctcaaacact ccgacacggc gatgtaccag gccaaggatc
     6541 tgggccggaa caacttccag gtgttctcgc cgcagatgga ccgcgccctc aaggagcgcg
     6601 tgacaacgga atcttccttg cgcgtcgcgc tcaagctcaa ccagttcgac gtgcactacc
     6661 agcccatcat cgacattcat tcgcggcggg tggccggcct cgaagccctg ttgcgctgga
     6721 aacatccgac gctcggcttc gtgccacccg accgcttcat cgaagtcgcc gaagagaccg
     6781 gcctgatcat tcccatcggc caattcgtgc tcgaccgcat cggcaccgac atcgccaagt
     6841 ggcgggagca gggcgcgcac ctggtgccgg tgagcatgaa cgtgtccgcg gtccagctcg
     6901 agcgcaccaa gctgcgcgag ctgatccagc aggccatgct caagaatcga atcggtccgc
     6961 agctgatcca gctcgagctc acggagggct cgctgttcga gacgcgcacc ggcgagttcc
     7021 gcgaagacgc gctggccacg ttgcgcgacc tgggcgtcaa gatcgccatc gacgacttcg
     7081 gcacgggtta ctcgagcttg tcgtatctca agcgctggcg cgtcgactcg ctgaaaatcg
     7141 atcgcagctt cgtgcgcgac atcgccaccg atccttccga ccacgccatc gtcagcgcga
     7201 tcgtcgccat ggcgagaagc ctcaacatcc aggtcgtggc cgaaggcatc gagacctggc
     7261 agcagctcga aatccttcgc aacatgggtt gcagtctcgc gcaggggttc ctgttcgcga
     7321 agccgtgccc ggcgtcggac gcgctgcgtt atctgcgcgt cgaaccactg gacctcctcg
     7381 aatcgaactg ggagtcctcc aactccctcg ccgaaactgg ctagctctga aatactccgg
     7441 cccataacgt aactacgttg caggtctgga ggattcatgt tgaaaggctg gacagggaag
     7501 gccgcgttcg cggctgcatt cgtcatcacc agtacgatcg tgctcgccgc cgataccgat
     7561 gattcgctgg gacgcgaaca actgcatgcc acgttgtgga tgcagacggc gccggagtat
     7621 cgggcgatat cgctgcaggt ataccgacag gccgaagagc gcatcgcggc gccgctgccc
     7681 ggcagcgcgg ccctcgaaca ggcagccgtt cccgccgaga cactggcccg gcttcccacg
     7741 gcggtggtgc tcgatctcga cgagaccgtc ctcgacaaca cggtctacca ggcccggttg
     7801 ctgcgcgacc gcgccaccta caacacgacg acctggggcg aatgggtggc cgccggcgag
     7861 gcggaagcat tgcccggttc gcgggagttc attgccaggg cgcgcgcgct cggccacacg
     7921 gtcttctaca tcagcaaccg cgactgcacc gcgccgccgc ccacggccgc ggatccctgt
     7981 ccggcgaaaa ccgccacgat gaaaaacctg gtggctctcg gaatcgaaga cgcgccggat
     8041 ccgaagcgca tgttgttgcg cggcgaacgt ccggagtgga acacgggcaa caagacccag
     8101 cggcgcgcgt tcatcgccgc taactaccgc atcgtcgcgc tggtcggcga cgacctgggc
     8161 gatttcaccg atccgcaggt tttcgccggc gaccgcgaac gcctcgagcc gcgattcggc
     8221 gccacctggt tcctgctgcc gaatcccatc tacggttcct ggacc
//
LOCUS       DATDEU010000053        12052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_154612, whole genome shotgun sequence.
ACCESSION   DATDEU010000053 DATDEU010000000
VERSION     DATDEU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154612"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VIV63_01130"
     CDS             <1..597
                     /locus_tag="VIV63_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123224.1"
                     /translation="ERHRQGSRDGRHPRLALGACVMFERLDSLDRVLETHSVALGGDL
                     NAYRNHAYRVVNLCAALAPCTDDSLEKVALASAFHDLGIWTAQTFDYLEPSVALACNH
                     LDCAGHGDWRDTVAGMIREHHKVRVFRGADAGFVEPFRRADWIDVSLGVLSFGLPRAR
                     LREIFRRWPNAGFHQRLAQLTFKRLRTHPLSPIPVLKW"
     gene            complement(587..1975)
                     /locus_tag="VIV63_01135"
     CDS             complement(587..1975)
                     /locus_tag="VIV63_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6123225.1"
                     /translation="MNATDATVPATAKLSKSLRARHLAMISIGGIIGAGVFVGSSTAI
                     STIGPAVVLSYIFAGIVVFMVIKILARMALDQPGLGAFTEYVRVALGGNAGFISGWLY
                     WYFWVIVVGIEAIAGAKIVHGWMPDFDVWQIGLALMVVLTLVNLTSARSYGEFEFWFA
                     SIKVAAIIVFIIVLAAWLFGVGGHTSPGLSNLTSQGGFAPLGYPVVFTGVTTVIFSMC
                     GAEIITVAAAESAESGKTMSRLAASVVLRILLFYVLAIFLIVCVLPWNAFKSGDSTFS
                     MVLEHIGIPYARLIMDCIVLTAVLSCLNSGVYVTARVMFAMADKGDAPRWLTAVNKRQ
                     VPARAILLGSAFGFISMFLNVGSDNESIAKLFTQLINASGAIMLIVYCFVALAHYRFP
                     YTAEAEKQSGSKRTADIVAGVAAFAILVVMSSMAFIKGMAEQLYPSLGLLAAILIALA
                     IKRLVEKRGLTT"
     gene            2036..3511
                     /locus_tag="VIV63_01140"
     CDS             2036..3511
                     /locus_tag="VIV63_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6123226.1"
                     /translation="MKRREFIGATLAGGAAAALPGTALAAAKSKPAGVPAVQLSGAAT
                     FLEEPALKQLAGTMRGSLILPTHEEYETARRVWNPMIDRRPALIARCAEAKDVVKAVN
                     FARERELLVAVRSGGHSFPGHSTCDGGMVIDLSSMRKVRVDAGRKTATVEAGAWVSDV
                     DSKTLPLGLATTMGQISDTGVAGLTLGGGYGWLSRKLGLACDNLIWADIVTPDGKFQR
                     VSANENEQLFWAIRGGGGNFGVATSFEYRLHKQQQQVYAAYISYPVEHLRAVMELYQE
                     TTATGPRDLSLDLSLEASDKGEPLVAVYACHSGDPAEGPALIAPFDRVAKPFQSVHGP
                     DTYERVQHQSDGPHLSKSLHYIKAGLVREFKPELIETLQREFRPSGEIFIYMQNASGA
                     VGDVAPQGTAFWNRKSMANLMILGTWRDPANTEKNRAAIRAAWEKIAPFTEGFYTNLS
                     DADAQSTHRNYGGNYARLSVLKRKFDPANMLRLNSNITPAI"
     gene            complement(3563..6559)
                     /locus_tag="VIV63_01145"
     CDS             complement(3563..6559)
                     /locus_tag="VIV63_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123227.1"
                     /translation="MSRSDCSSSFVLRRAISLVLSGAALALPLQAIAQDAASGAAETQ
                     ELEEVVVTGFRGSLNTAIAAKRSETAAIDVIASEDIGKFPDSNLAESMQRVPGVALSR
                     SDGGEGRNISVRGLAAGFTKVRINGMEGAAQTGSSDIYGAGNNGRAFDFNVFPTEIFS
                     QLSVRKTASADIEEGSLGATVDLKAPGAFDYDDDQVFSITGRGIYNEVAENLDPRVSM
                     LASKKFFDETFGVLATFSFQERHTREVGYSAVDILHFGTNANNIGTATAPILLPFCTP
                     IGWTLTAPSPVPGTRGATGTDCSTGNGRTSDMAAFMEVYNRTNASIRDAAGNLVPGGG
                     AFHPRLPRFVNSEQDTERSGGSLSLQWRPNENTSVSLDGLLSRYQQERRDNYILGLSF
                     GRNLSNNGQPMVSIRDVEFDEDGSMIYGLFDGVDVRSEGLVDQFVSKFEQLSLDVEHQ
                     INDSFGVHFYAGRSVNTWDGPLRFQTFMDAIDTDNFSVDFRGGGSVPRIGFGFDVSDP
                     NSFRYAPTPDGNQTVLGGFSLQGKPQQNITANNTFDLDGTWQMSDAWKVKLGVQYRES
                     HFSSHGSNPLRNATLTRALPAGVTIADLTTQITDLDSLFGANAPASWAAIDIKKWDEV
                     FDIDSVLTCEGICGAAQSKIFEDVSTGYIMFGFDSGDSWGIPIRGDIGVRYVMTDQFA
                     SGIIPTSAPPTSIYTSFGAYNEVERDYNDTLPAMNVVFELSDNLLARLSASKVMSRPE
                     LGNLAPTAGVTATTRTGNVNNPFLDPIRADTADLALEWYFAEGSLLSVAYFYKDIETY
                     IQRITEQVPYRDLGLPDSLLDGTPASPTDIFTVGRFQNTPGGELTGFEINAQVQFNFL
                     PSFWSNFGVLANYTKVDADITYFISATAPPVTAQLVGMSPTTASGTLFYENEKFSVRT
                     TASYRDKYFRAIPASPGSDVRGDLSTTYVDAQASWFVTDNLSLILEAQNLTGERSTLY
                     IDDTRENTLFETELGTTYTFGATYKV"
     gene            complement(6890..7144)
                     /locus_tag="VIV63_01150"
     CDS             complement(6890..7144)
                     /locus_tag="VIV63_01150"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HEY6123228.1"
                     /translation="MIDPEWIGYTAAALTTASFIPQAVMTIRSRDTRGISRGMYIIFT
                     IGVALWLAYGVYIDSLPMILANTVTLALAATILALKLRYG"
     gene            complement(7152..9146)
                     /locus_tag="VIV63_01155"
     CDS             complement(7152..9146)
                     /locus_tag="VIV63_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY6123229.1"
                     /translation="MRKRPAVLFALLVLTIGAATGYHVISRQREADAIQRTTPSRPAA
                     KPATPTQVRETHPRIDDSADILAPFVPRLGRMADAFNTDLGVDVRVVTSTDGSTPIET
                     QAVQTFSQREIGREAPAGGLLVILDPKLKSARIEVGYALEGVMTDLQMGRLARDQLAP
                     YVAYGVAGMAVMDVMQHLRERALLAAAQGEIDIPDAARELPEYLRYKQYLSGGAGAAT
                     ALAKLPADADLKKPIPAGQRARYAPAETPEGSVAALLRAMADFAGDPTLELYTEGSQL
                     QRANYPLAPFEELQRLERVQRSKPLHYWIQGDRAVASSKKPVRGFVPILLHRQGGLWR
                     VDLVETWKNLFFDPDGNYFLRNSNTPYAAGLREFGLGGYYDLAALRLRAPSIAAEISQ
                     LEQRTDVLSTLRRGELWLRNGFVFPRAWLAYEEARRKAPKDPLVLETIGSRAQYLGFP
                     EIAIPALEQIGRGLELTIAAAYDEMGDPEGAQRWVERALAENPFDLYALRWREHLAKR
                     HGTDAELQEARRELSAALDDRFRARDPVVLSFHPDEPGFETRSTINVGGTTVYDHSHF
                     GVTLRNTSGREVEIESVQLGSQGTASTSGLGDVRNYWTFPRGRNRLGAGEAVYFDKQW
                     GFVVDTAHEHVRYIFRLCWHAVTDPDPVRQCRTQWVDALP"
     gene            complement(9164..9239)
                     /locus_tag="VIV63_01160"
     tRNA            complement(9164..9239)
                     /locus_tag="VIV63_01160"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9204..9206),aa:Phe,seq:gaa)
     gene            9351..10214
                     /locus_tag="VIV63_01165"
     CDS             9351..10214
                     /locus_tag="VIV63_01165"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY6123230.1"
                     /translation="MNTAARLATTPRAVIQTLRGQPASDGAGVKLTRVIGQPKLPDLD
                     PFLLLDEFGTDKAEDYIAGFPEHPHRGFETVTYMLDGRMRHKDNHGNEGVLEPGAVQW
                     MTAGRGIVHSEMPEQREGRMRGFQLWMNLPAKDKMIAPNYQEFGGDKLPVVERPGVNV
                     KIIAGSLDGATGPVRQPATDPVYMDIRLDAGVDFALPLPVGHSAFVYVYEGGVSVGAS
                     REAATIHAQELAVLGDGNEVLLQGRAARSRAILVAGRPLREPVARYGPFVMNTREELH
                     QAFADFQAGKF"
     gene            complement(10263..10532)
                     /locus_tag="VIV63_01170"
     CDS             complement(10263..10532)
                     /locus_tag="VIV63_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY6123231.1"
                     /translation="MTRMVNCILLKKEAPGLDRPPYPGELGKRIFENVSKEAWQQWVK
                     HQVMLINEYRLTPIEPAHRKRLEFEMEKFFFGEGSSKPAEFVEKQ"
     gene            complement(10529..11602)
                     /gene="mutY"
                     /locus_tag="VIV63_01175"
     CDS             complement(10529..11602)
                     /gene="mutY"
                     /locus_tag="VIV63_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY6123232.1"
                     /translation="MSVGAKGIAPRLLAWHSLKGRHDLPWQKDLEKKGSSYRVWVSEI
                     MLQQTQVATVIPYFERFMKRFPDVRSLADAPADEVLHLWTGLGYYARARNLHRAAQII
                     RDRHDGQFPQEIDAVIELPGIGRSTAGAILSISAGSRHPILDGNVKRVLSRFYAVDGA
                     PDESATIEKLWRLADDNTPSKNVGVYTQAIMDLGATLCTRTKPRCEDCPIARDCRARI
                     EGRQDALPAAKRKRAPRKLRHAVMLVARRATEVLLVQRPPSGIWGGLWCLPEFEHRDA
                     AEEYAASTLPSAKLASAPLPDIAHSFTHFDLVITPVVAQCRGEARVRDGGALWYDLTR
                     PARVGLPAPIRTLLGTLLERNES"
     gene            complement(11599..>12052)
                     /locus_tag="VIV63_01180"
     CDS             complement(11599..>12052)
                     /locus_tag="VIV63_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123233.1"
                     /translation="TFDPRALLTSVGIAPPKTTDPAALGKVRFAGGWAFDNGAIRIDP
                     FTLDLDETHFQGSFSRTAGEDALGEFALAGNSIDIARYIPPSDPASEPFVLPTVTLKA
                     LRFRGEMKLEHATLDDIDMKQVTLRLLLDEQGLRSVPKPAATPLPEKQ"
BASE COUNT         2257 a         3766 c         3897 g         2132 t
ORIGIN      
        1 gagcgacatc ggcaaggaag ccgtgatggc cgacatccgc gcctggctct cggcgcatgt
       61 gtgatgttcg agcggctcga ttcactcgat cgcgtgctcg aaacgcattc ggttgcgctg
      121 ggcggcgatc taaacgcgta tcgcaaccat gcctatcgcg tcgtgaatct ttgcgcggcg
      181 cttgcaccct gcaccgatga ttcgctcgag aaggtagcgc tggcgtcggc gttccacgac
      241 ctcggcatct ggaccgcgca gacgttcgac tacctggaac cttcggtggc gctcgcgtgc
      301 aatcacctgg actgcgcggg acatggcgac tggcgtgaca ccgttgccgg catgatccgc
      361 gagcatcaca aggtccgcgt gtttcgtggc gctgacgccg gattcgtcga gccattccgg
      421 cgcgcggact ggatcgacgt gtcgctgggc gtgttgtctt tcgggctgcc tcgcgcgcgt
      481 ctgcgcgaga tcttccggcg atggccgaac gccggctttc atcaacggct ggcgcagctc
      541 accttcaaac gattgcgcac acatccattg agtccgatcc cggttctcaa gtggtgaggc
      601 cgcgcttctc caccagacgc ttgatcgcga gcgcgatcag gattgccgcg agcagaccca
      661 gactggggta gagctgctcc gccatgccct tgatgaaggc catagacgac atgacgacga
      721 ggatggcgaa cgcggcgact ccggccacga tgtcggcagt ccgttttgag cccgactgtt
      781 tctccgcttc cgccgtgtac gggaaccggt aatgcgccag cgcgacgaaa caatagacga
      841 tcagcatgat cgcgccggac gcgttgatga gctgcgtgaa cagcttcgcg atgctttcgt
      901 tgtcggagcc gacgttgagg aacatcgaga tgaacccgaa cgcgctgccc agcaggatgg
      961 cgcgtgccgg aacctgccgc ttgttgacgg ccgtgagcca gcgcggcgcg tcgcccttgt
     1021 ccgccatggc gaacatcacg cgcgcggtga catacacgcc cgagttcagg cacgacagga
     1081 ccgccgtcaa cacgatgcag tccatgatga ggcgcgcata cggaatgccg atgtgttcga
     1141 gcaccatcga aaacgtggag tcgccggact tgaaggcatt ccacggcagc acgcagacga
     1201 tgaggaagat ggcgagcacg tagaacagca ggatgcgcag cactacgctc gcggccagcc
     1261 gcgacatggt cttgcccgac tccgccgatt cggcggcggc gacggtgatg atctccgcgc
     1321 cgcacatcga aaagatgacc gtggtcacgc ccgtgaaaac caccgggtaa ccgagaggcg
     1381 cgaagccgcc ctgtgacgtg aggttcgaaa gccccgggct ggtatgtccg ccgacgccga
     1441 acaaccatgc cgcgagcacg atgatgaaga cgatgatcgc ggcgaccttg atggacgcga
     1501 accagaactc gaattcgccg tatgaacggg ccgaggtgag attgaccagc gtgagcacga
     1561 ccatgagcgc gaggccgatc tgccagacgt cgaagtccgg catccagccg tgcacgatct
     1621 tcgcgccggc aatggcttcg atgccgacga cgatcaccca gaaataccag tacaaccagc
     1681 cgctgatgaa acccgcattt ccgccgaggg cgacgcgcac gtattcggtg aacgctccga
     1741 gcccgggctg atcgagcgcc atgcgcgcca ggatcttgat gaccatgaac acgacgatgc
     1801 cggcgaagat gtagctgagc accacggcgg gcccgatcgt cgaaatggcc gtgctgctgc
     1861 cgacgaacac accggcgcct atgatcccgc cgatggagat catcgcgaga tggcgggcgc
     1921 gcagggactt ggataacttg gcggtggcgg gaacggtagc gtcggtggcg ttcaagtttg
     1981 ttcgctcttg ttgtctcgtg acgacggagc gcaccatacg ccgaggagca gaaaaatgaa
     2041 aagaagagag ttcatcgggg cgacgctcgc gggcggcgcg gcggcggcgt tacctggcac
     2101 ggcgctggcg gcagccaaat cgaaacccgc cggcgttccg gcggtgcagc tttccggcgc
     2161 ggccacgttc ctcgaagagc cggcgctcaa gcagcttgcc ggcaccatgc gcggctcgct
     2221 catcctgccg acgcatgagg aatacgaaac ggcgcgacgc gtctggaatc ccatgatcga
     2281 ccggcggccg gcactcatcg cgagatgcgc ggaagcgaaa gacgtggtga aagccgtcaa
     2341 ctttgcccgc gaacgcgaac tgctggtggc ggtgcgcagc ggcggccaca gtttccccgg
     2401 ccactccacc tgcgacggcg gaatggtcat cgatctttct tcgatgcgta aagtccgcgt
     2461 cgacgccggc aggaaaacgg ccactgtcga agccggcgcg tgggtcagcg acgtcgacag
     2521 caagaccctg ccactcggac tcgcgaccac catgggccag atttcggaca caggcgtcgc
     2581 cggcctgacg ctgggcggcg gctacggctg gttgtcgcga aagctcggac tcgcctgtga
     2641 caacctgatc tgggccgaca tcgtcacgcc cgacggaaaa tttcaacgcg tcagcgccaa
     2701 cgagaacgag cagctgttct gggccattcg cggcggcggc ggcaacttcg gcgtggccac
     2761 ttcgttcgaa taccgcctgc acaaacaaca gcagcaggtg tacgccgcgt acatctccta
     2821 tccagtcgaa cacctgcgcg cagtcatgga gctctaccag gagacgaccg caacgggtcc
     2881 gcgagacctg agtctcgacc tcagcctcga agcctcggac aagggcgagc cgctcgtggc
     2941 cgtctatgcg tgtcactcgg gtgatcccgc cgaagggccc gcgttgatcg cgccgttcga
     3001 tcgcgtagcc aagccgtttc aaagcgtcca tggcccggac acctatgaac gggtccaaca
     3061 ccagtccgac ggtcctcacc tgtcgaagag cctgcactac atcaaggccg gtctcgtccg
     3121 ggagttcaaa cccgagctca tcgaaacgct gcaacgcgag ttccggccca gcggcgagat
     3181 cttcatctac atgcagaacg ccagtggcgc ggtgggcgac gtggcgccgc agggcacggc
     3241 attctggaac cggaagagca tggcgaatct catgatcctg ggtacctgga gagatccggc
     3301 caacacggag aagaatcgcg cggccattcg cgcggcgtgg gagaagatcg cgccgttcac
     3361 cgagggtttc tataccaacc tcagcgacgc cgatgcgcag agcactcacc gcaattacgg
     3421 cggcaactac gcgcggctca gcgttctcaa gagaaagttc gatccggcga acatgctgcg
     3481 tctgaattcc aacatcacac cggcgatctg acgcgcaaaa aaaagcggcc gcctgggaag
     3541 gcggccgctc tcatcatcag cttcagacct tgtacgtcgc gccgaacgtg tacgtggtcc
     3601 cgagctcggt ctcgaacaac gtgttctcac gcgtgtcgtc gatgtacagc gtgctgcgct
     3661 cacccgtgag gttctgggcc tcgaggatca gcgacaggtt gtcggtcacg aaccaggacg
     3721 cctgcgcatc cacatacgtc gtcgacaggt cgccgcgcac gtcgctgccc ggcgaggcag
     3781 ggatcgcacg gaagtacttg tcgcgatacg aggccgtcgt gcgcacgctg aacttctcgt
     3841 tctcgtagaa caacgtgccg ctcgccgtcg tcggtgacat gcctaccagc tgcgccgtca
     3901 ccggcggagc ggtcgcgctg atgaagtacg tgatgtccgc atccaccttc gtgtagttgg
     3961 ccagcacacc gaagttgctc cagaagctcg gcaggaagtt gaactgcacc tgcgcgttga
     4021 tctcgaagcc cgtcagctca ccgccgggcg tgttctggaa gcgacccacg gtgaagatgt
     4081 cggtgggtga cgccggcgtg ccgtccagca gcgagtcggg caggccgaga tcgcgatacg
     4141 ggacctgctc ggtgatgcgc tggatgtacg tctcgatgtc cttgtagaag taggccaccg
     4201 acagcaacga gccttccgcg aaataccatt ccagggccag gtccgccgtg tcggcgcgga
     4261 tcggatcgag gaacggattg ttcacgttgc ccgtgcgcgt ggtcgcggtg acacccgcgg
     4321 tcggcgccag gttgcccagc tccggtcgcg acatcacctt cgaggcggag aggcgcgcga
     4381 gcagattgtc gctgagctcg aacaccacgt tcatcgccgg cagtgtgtcg ttgtagtcac
     4441 gctccacttc gttgtacgcg ccaaacgacg tgtagataga agtgggcggc gccgaggtcg
     4501 ggatgatgcc cgacgcgaac tggtcggtca tcacgtaacg aacgccgatg tcgccgcgga
     4561 tcgggatgcc ccagctatcg ccagagtcga agccgaacat gatgtaaccc gtcgacacgt
     4621 cttcgaagat cttgctctgt gcggcaccgc aaatgccttc gcaggtcagg acgctgtcga
     4681 tgtcgaacac ttcgtcccac ttcttgatgt cgatcgccgc ccagctcgcc ggtgcgttcg
     4741 cgccgaacag gctgtcgaga tcggtgatct gcgtcgtgag gtccgcgatg gtcacgcccg
     4801 cgggcaacgc ccgcgtaagg gtggcgtttc gcaacgggtt cgagccatgg ctgctgaagt
     4861 ggctctcgcg atactgcacg ccgagcttca ccttccacgc atcgctcatc tgccaggtgc
     4921 cgtccaggtc gaacgtgttg ttggccgtga tgttctgctg cggcttgccc tgcagcgaga
     4981 aaccacccag cacagtctgg ttgccatcgg gcgtcggggc ataacggaag ctgttgggat
     5041 ccgacacgtc gaacccgaag ccgatgcgcg gcacactgcc gccaccgcgg aagtcgaccg
     5101 agaagttgtc ggtgtcgatc gcatccatga acgtctggaa gcgcagcgga ccatcccagg
     5161 tgttgaccga gcggccggcg tagaaatgca cgccgaagct gtcgttgatc tggtgttcga
     5221 catcgaggct caactgctcg aacttcgaga cgaactgatc gaccaggcct tctgagcgca
     5281 cgtcgactcc atcgaacagg ccgtagatca tcgagccgtc ttcgtcgaac tcgacgtcgc
     5341 ggatcgacac catcggctgg ccgttgttgc tgaggttgcg gccgaacgag aggccgagga
     5401 tgtagttgtc gcgtcgttcc tgctggtagc gcgagagcaa gccgtcgaga gagacggagg
     5461 tgttctcatt gggacgccac tgcagcgaga ggctgccgcc cgaacgctcg gtgtcctgct
     5521 cggaattcac gaagcgcggc aggcgcggat ggaaggcgcc accaccgggg acgagattgc
     5581 ccgcggcatc gcggatcgac gcgttggtcc ggttgtagac ctccatgaac gcggccatgt
     5641 cgctggtgcg accgttgccg gtgctgcaat cggtgcccgt ggcgccgcgc gtacccggta
     5701 cgggactcgg agcggtgagc gtccagccga tgggtgtgca gaacggcaac aggatgggcg
     5761 ccgtggcagt gccgatgttg ttggcgttcg taccgaagtg gaggatgtcc accgccgagt
     5821 aacccacttc acgcgtatgc cgttcctgga aagagaacgt ggccagcacg ccgaaggtct
     5881 cgtcgaagaa cttcttcgac gccagcatcg acacgcgcgg atccagattc tcggcaactt
     5941 cgttgtagat gccgcggccg gtgatcgaga acacctggtc gtcgtcgtag tcgaaagcgc
     6001 ccggcgcctt gagatcgacc gtggcgccca gcgagccttc ctcgatatcc gccgacgccg
     6061 tcttgcgcac ggaaagctgc gagaagattt cggtcgggaa aacgttgaag tcgaaagcgc
     6121 ggccgttgtt gccggcgcca tagatgtcgc tggagccggt ctgcgcggca ccttccatgc
     6181 cgttgatgcg gaccttggtg aaacccgcgg cgaggccgcg gaccgagatg ttgcggcctt
     6241 cgccaccgtc gctgcgcgag agcgcgacgc cggggacgcg ctgcatggat tcggcgaggt
     6301 tgctgtcagg gaacttgccg atgtcctccg acgcaatgac gtcgatggcg gcagtctcac
     6361 tacgcttggc agcgatcgcg gtattcagcg aaccgcggaa gccggttact acgacctctt
     6421 cgagttcctg agtttctgct gcgccactcg cggcatcctg cgcgatcgcc tgcagcggga
     6481 gcgcgagagc cgcgccggaa agcaccagcg atatcgcgcg gcgcaaaaca aaagaagatg
     6541 agcagtcgga cctggacatt gaaattcccc cctgagccgt ggtttcgagt tgaatctgaa
     6601 aaaccgcgcg gtcaaccaat cacgcctggg ccacggacac gccttcagct gatcggattt
     6661 gaccgattta tactgtgtat gacgctgatt gatccctgta attgacgcca agtcaagtag
     6721 attgtccacc ggtggcaacg gttgttgcga tcaagcaacg aacggatcaa ctctttgaat
     6781 agagaggtgt ggaattagcg cttcgcgtgc ttttaccgcc tttttcgggc gagcgcgaga
     6841 tccacgacct gtgatcagtg gatatcaaga ccggcatctg aaagggacgt cagccgtaac
     6901 gcagtttgag cgccaggatg gtagccgcga gcgcgagcgt caccgtgttg gcgaggatca
     6961 tcggcaggga gtcgatgtag accccatagg cgagccagag cgccacgccg atggtgaaga
     7021 tgatgtacat gccgcgcgag attccacgcg tgtcgcgcga ccggatggtc atcaccgcct
     7081 gagggatgaa agaggcggtg gtcagcgccg cggcggtata gccaatccat tctgggtcga
     7141 tcatggcgtc actatggcag ggcgtcgacc cactgtgtcc tgcactggcg cactggatcc
     7201 gggtcggtca cggcatgcca gcagagccgg aatatatagc gcacgtgctc gtgggcggtg
     7261 tcgacgacga agccccattg cttgtcgaag tacacggcct cgccggctcc caaccggttc
     7321 ctgcctcggg gaaacgtcca gtaattccgc acgtcgccga gtccgctcgt ggaggctgtc
     7381 ccctggctcc cgagctggac gctttcgatc tccacctcac ggccactcgt gttcctgagt
     7441 gtgactccga aatggctgtg atcgtagaca gtcgtgccgc caacgttgat cgtagagcgt
     7501 gtttcaaagc cgggctcgtc gggatggaat gacaacacga ccggatcgcg ggcacgaaat
     7561 cggtcgtcga gcgcagccga caattcacgg cgagcctcct gtagctccgc gtctgtgccg
     7621 tgacgtttcg caagatgttc gcgccatcgc aaggcgtaca gatcgaacgg attctcggcg
     7681 agcgcccgtt cgacccagcg ctgcgccccc tcggggtcgc ccatctcgtc gtaggccgcg
     7741 gcaatggtca gctcgagccc cctgccgatc tgctcgagcg ccggaatcgc gatctcggga
     7801 aaacccaggt actgcgcgcg cgacccgatc gtttcgagca ccaacggatc tttcggagcc
     7861 ttccgccgcg cttcttcgta ggccagccac gcccggggga aaacaaaccc attgcgcagc
     7921 cagagctcgc cgcgccgcaa tgtcgagagc acgtccgtac gttgttcgag ctgtgagatt
     7981 tcagcggcga tcgacggggc gcgaagtcgc aacgcggcca ggtcgtagta gcccccaagg
     8041 ccgaattccc tgagacccgc cgcgtacggc gtgttcgaat tccgcaggaa gtagtttccg
     8101 tccgggtcga agaacaggtt tttccaggtc tccaccagat cgacgcgcca cagcccgcct
     8161 tgccggtgca gcaggatggg cacgaaaccc cgcacgggtt tcttgctcga agccacggca
     8221 cggtctccct ggatccagta gtgcagaggc ttcgatctct ggacgcgctc gagacgctgc
     8281 agctcctcga acggcgcgag cgggtagtta gcgcgctgaa gttgcgagcc ttcggtgtag
     8341 agctcgagcg tcgggtctcc ggcgaaatcg gccattgccc gcagcaaggc cgcgaccgac
     8401 ccttccggtg tctccgcggg cgcgtaccgg gcgcgctgtc ccgccggaat gggctttttc
     8461 aggtccgcat cggcaggcag cttcgccagc gcggtcgccg cgcccgcccc gccggaaaga
     8521 tactgcttgt atcgaaggta ttcgggcagc tcgcgagccg cgtccggaat gtcgatctcg
     8581 ccttgggcgg ccgcgagtaa tgcccgctcg cgcaaatgct gcatgacgtc cattacggcc
     8641 atgccggcga caccgtaggc cacgtagggc gcgagctgat cgcgcgccag ccggcccatc
     8701 tgcagatcgg tcatcacgcc ttcgagcgcg taaccgactt cgattcgggc acttttgagt
     8761 ttcgggtcga gaatgacgag caagccgccc gccggcgctt cacgcccgat ttcacgctgg
     8821 ctgaaagtct ggacggcctg cgtttcgatg ggcgtcgagc cgtcggtgct cgtgaccacg
     8881 cgaacatcga cgccgagatc cgtgttgaac gcgtctgcca tgcgcccgag acgggggacg
     8941 aacggcgcca ggatgtcggc ggagtcatcg atgcgcgggt gagtctcgcg tacctgagtc
     9001 ggggtcgcag gcttcgcggc ggggcggctg ggcgtggtcc gctggatggc atcggcctcg
     9061 cgctgccggg aaatcacgtg atagcccgtg gctgcgccga tggtgaggac caggagtgcg
     9121 aaaaggactg ccggacgttt gcgcaacgaa aggccttcga aaatggtggc cggggtcgga
     9181 atcgaaccaa cgacacgcgg attttcaatc cgctgctcta ccaactgagc tacccggcca
     9241 cagtgggaat tcgagggggc gcgtattaga ccggccagcc ccggaggcgt caagcaagca
     9301 ggggctcaaa cccttgattt gcgacgcatt gacaccattt cgggccgacc atgaataccg
     9361 cagccagact cgccaccacc ccgcgggccg tcatccagac gcttcgcggc cagcccgcct
     9421 ccgacggagc gggcgtcaag ctcacgcgtg tcatcggaca gcccaagctg cccgatctcg
     9481 acccgttcct gctgctcgac gaattcggca cggacaaggc cgaagactac atcgccggat
     9541 ttccggaaca cccgcatcgg ggcttcgaga cggtgacgta catgctcgac ggccgcatgc
     9601 gccacaagga caaccacggc aatgaaggcg tgctcgagcc gggcgcggtg cagtggatga
     9661 ccgcgggccg cggcatcgtg cactcggaaa tgcccgagca gcgcgaagga cgcatgcgcg
     9721 gcttccagct gtggatgaac ctgcccgcga aggacaagat gatcgcgcct aactatcagg
     9781 agttcggcgg cgacaaactg cccgtggtcg aacgtccggg cgtgaacgtg aagatcatcg
     9841 cgggttcgct cgatggcgcc accggcccgg tgcgccagcc ggccacggat ccggtctata
     9901 tggacatccg gctcgacgcg ggcgtggact tcgcgctgcc gctgccggtg ggccattccg
     9961 ccttcgtata tgtgtacgaa ggcggggtgt cggtgggagc ctcgcgcgag gcggccacca
    10021 tccatgcgca ggaactcgcg gtgctcggtg acggcaacga agtgctgctg cagggacgtg
    10081 cagcgcgctc gcgcgccatc ctcgtcgccg ggcggccgct gcgcgaaccg gtggcgcgtt
    10141 atggcccgtt cgtgatgaac acgcgcgagg aactgcacca ggccttcgcg gatttccagg
    10201 ccggcaagtt ctgagtgacc cggtgatcga aaggacggcg caggaaactg cgccgtcctt
    10261 ttttattgct tctcgacgaa ctcggcgggc ttcgacgaac cctcgccgaa gaagaacttc
    10321 tccatctcga actcgagccg cttgcgatgc gcgggctcga tcggcgtcag ccggtactcg
    10381 ttgatgagca tgacctggtg cttcacccac tgctgccagg cctccttcga cacattctcg
    10441 aagatccgtt tgccgagctc gcccggataa ggcggccggt cgaggccggg cgcctctttc
    10501 ttgagcagta tgcaattcac catgcgggtc atgattcgtt tctctctaac aaggttccaa
    10561 gcagcgtccg gatgggcgcg ggcagtccga cgcgtgccgg gcgcgtgagg tcataccaca
    10621 gcgcgccgcc gtcgcgcacg cgagcctcgc cgcggcattg cgcgaccacc ggcgtgatca
    10681 cgagatcgaa gtgcgtgaag ctgtgggcga tatcgggcag cggcgcactc gccagcttcg
    10741 cgctggggag cgtggacgca gcgtattcct cggcggcatc gcgatgttcg aattccggca
    10801 gacaccacaa cccgccccag atcccgcttg gcgggcgctg caccagcaac acttcggtgg
    10861 cgcggcgcgc caccagcatg acggcgtgcc ggagtttgcg cggggcgcgc ttgcgtttgg
    10921 cggccggcag agcgtcttgc cgcccctcga tgcgggcccg gcaatcacgg gcaatcgggc
    10981 aatcctcaca acgcggcttg gtgcgcgtac acaaggtggc gcccagatcc atgatggcct
    11041 gcgtgtagac gccgacattt ttcgaaggcg tgttgtcgtc cgccagacgc cagagcttct
    11101 cgatcgtggc gctttcgtcc ggcgcaccgt caaccgcgta gaagcgcgaa agcacacgct
    11161 tcacgttgcc gtcgagaatc ggatgcctgc tgcccgcgga gatggagaga atcgcgccgg
    11221 ccgtcgaacg cccgatgccc ggcagttcga tcaccgcgtc gatctcctgc ggaaactgtc
    11281 cgtcatgacg atcgcgaatg atctgggccg cgcgatgcag gttgcgtgcg cgcgcgtagt
    11341 agccaagccc ggtccacagg tgcagcactt catccgccgg cgcgtcggcc agcgagcgca
    11401 cgtccgggaa tcgtttcatg aagcgctcga aatacgggat gaccgtggcc acctgtgtct
    11461 gctgcaacat gatttcggag acccatacgc gatacgagct gcccttcttc tcgagatcct
    11521 tttgccaggg cagatcgtga cgacccttca gcgaatgcca ggcgagcagc cgcggcgcaa
    11581 tgcctttcgc gcccacgctc attgtttttc cggaagcggc gtcgcggccg gcttgggcac
    11641 gctgcgcagt ccctgctcat cgagcagcag ccgcagcgtc acctgtttca tgtcgatgtc
    11701 gtcgagcgtc gcgtgttcga gcttcatctc accgcgaaag cgcaatgcct tcaatgtcac
    11761 ggtgggaagc acgaaaggct cgctcgccgg atcgctgggt ggaatgtagc gtgcgatgtc
    11821 gatcgaattt ccggccagcg cgaattcacc gagtgcgtcc tcgccggcgg tccggctgaa
    11881 cgagccctgg aagtgggttt cgtcgagatc gagcgtgaat gggtcgatgc ggatcgcacc
    11941 gttgtcgaaa gcccagccgc ccgcgaaccg caccttgccg agcgccgcag ggtcagtggt
    12001 tttcggtggc gcgatgccga ccgaagtgag cagcgcgcgc gggtcgaagg tg
//
LOCUS       DATDEU010000054         2765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1468005, whole genome shotgun sequence.
ACCESSION   DATDEU010000054 DATDEU010000000
VERSION     DATDEU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468005"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VIV63_01185"
     CDS             <1..473
                     /locus_tag="VIV63_01185"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY6123234.1"
                     /translation="DSGRQYDGEGNLRDWWTADDGAKFKQRAGELVKQFAGYSVLDGR
                     HINGELTLGENIGDLSGMAVAFKAYKLSLAGKPAPEIDGFTGDQRFFLGWAQVWRRNY
                     RDAELLKRLVTDPHSPSEFRANGPSSNIDAFYDAFAVKPGDLMYRAPNERVKIW"
     gene            485..1141
                     /locus_tag="VIV63_01190"
     CDS             485..1141
                     /locus_tag="VIV63_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY6123235.1"
                     /translation="MRKLMLSFIVVLASANLAGCGYNKLQNQDEGVKAAWSEVVNQYQ
                     RRADLIPNLVAAVKGFAAQEERVLVGVTEARSKATSIQLTPELINDPAAFAKFQAAQG
                     ELTQALKSLIAVTENYPELKSDANFRDLQVQLEGTENRIAVARNRYIDAVKQFNVTVR
                     EFPTNLTAKMFDFDVKNNFTVENEAAISTAPAVNFDTSTPSQSATPVEAAPTTPPPAN
                     "
     gene            1150..2031
                     /locus_tag="VIV63_01195"
     CDS             1150..2031
                     /locus_tag="VIV63_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY6123236.1"
                     /translation="MKAALITLALLLAGFAPAAQAQVPVPPFEALVVDLTGTLTAVQQ
                     SQLDEKLTAFQARKGAQVALLIVPTTEPEDIAQYGIRVAEAWKVGRGPTDDGAILIVA
                     KDDRELRIEVGHGLEGALTDITSSRIINDTIVPLFKQGDFSGGINAGVDQMLRVIDGE
                     PLPEPDQAWKPGGDGGTSLPFLLMAGFIALNFMRSFVGRAPLAAIAGLGGGFLTWWLT
                     SKIPLSAGIGVGLFLLTLSLSGRGGSGRGGSGRVFRDIARGGGLGGLGGGFGGRGGGF
                     GGGGGGSFGGGGASGRW"
     gene            2039..2533
                     /locus_tag="VIV63_01200"
     CDS             2039..2533
                     /locus_tag="VIV63_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY6123237.1"
                     /translation="MELMRMLRHLCATRAGTRRRFTDEVDAVIETAIRAAESRTSAEI
                     RFVIETALDIPELWAGVSPRDRACQVFSQFGVWDTELRNGALIYVLAADRDVEIVVDR
                     GAFRISPVEWEAACKLMEGHFRAGRFQEGAVAGVEAVGGLLAREFPVRSGDRDELPNQ
                     PSLL"
     gene            2581..>2765
                     /locus_tag="VIV63_01205"
     CDS             2581..>2765
                     /locus_tag="VIV63_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123238.1"
                     /translation="MKTHVALRPLVVPALVFALLSGCSLLGEGPKWGAKRDADKAANA
                     VPQIPLAVPTHRFEIDET"
BASE COUNT          501 a          858 c          917 g          489 t
ORIGIN      
        1 acgattccgg acggcagtac gacggcgagg gaaacctgcg tgactggtgg accgcggatg
       61 acggagcgaa attcaagcag cgcgcgggcg aactggtgaa gcagttcgcc ggttacagcg
      121 tgctcgatgg ccggcatatc aacggcgagc tcacgctcgg cgagaacatc ggggacctgt
      181 ccggcatggc ggtcgccttc aaggcctaca agttgtcgct ggccggcaag cccgcgcccg
      241 agatcgacgg cttcaccggc gaccagcggt tcttcctcgg ctgggcgcaa gtctggcgcc
      301 gcaattaccg cgacgctgaa ctgctgaagc gactggtgac cgatccgcac agcccgagcg
      361 agtttcgtgc gaatggtccg tcaagcaata tcgacgcttt ctacgatgcc tttgccgtaa
      421 aacccggcga tctcatgtat cgcgcgccca acgagcgtgt gaagatctgg tgaaggggaa
      481 aatcatgcgc aaactgatgc tgagtttcat cgtcgtgctc gcgagcgcca atctcgcggg
      541 ttgtggttat aacaagctgc agaaccagga cgagggcgtg aaggccgcgt ggtctgaagt
      601 cgtcaatcaa taccagcggc gcgcggacct cattccgaat ctcgtcgccg cggtgaaagg
      661 tttcgccgcg caggaagaac gcgtgctggt cggcgtaacc gaagcgcgct ccaaggccac
      721 ttccatccag ctcacgcccg agctcatcaa cgatccggcg gcgttcgcca agttccaggc
      781 ggcgcagggc gaactcacgc aggcgctcaa gagcctgatc gccgtcaccg agaattatcc
      841 ggagctcaag tcggacgcga acttccgcga cctgcaggtg cagctggaag gaaccgagaa
      901 ccgcatcgcc gtcgcgcgca atcgctacat cgatgcggtc aagcagttca acgtgacggt
      961 tcgcgagttc cccacgaatc tcaccgcgaa gatgttcgac ttcgacgtga agaacaattt
     1021 cacggtggag aacgaagcgg cgatctccac ggcgccggcg gtgaatttcg acacctccac
     1081 gccatcgcaa tcggccaccc cggtagaagc ggcgcccacc acacctccgc cggcgaactg
     1141 accggcagca tgaaagctgc gctgatcacg ctggcgctgc tgctggccgg tttcgcgccg
     1201 gcggcgcaag cgcaggttcc cgtgccgccg ttcgaagcgc tggtggtcga tctcaccggc
     1261 acgctgacag ccgtgcagca gtcgcagctc gatgaaaagc tgacagcgtt ccaggcgagg
     1321 aaaggcgcgc aggtggcgtt gctgatcgtc ccgaccaccg agcccgaaga cattgcgcaa
     1381 tacggcatcc gggtcgcgga agcgtggaag gtcggacgtg gcccgaccga tgacggtgcg
     1441 atcctcatcg ttgccaagga cgaccgcgaa ctgcgaattg aagtggggca tggcctggaa
     1501 ggcgcgctga ccgacatcac gagcagccgc atcatcaacg acaccatcgt gccgttgttc
     1561 aagcagggcg atttctccgg cggcatcaat gccggtgtcg accagatgtt gcgcgtcatc
     1621 gacggcgagc cgttgccgga accggaccag gcctggaagc cgggaggaga tggcggaacg
     1681 tcgctgcctt tcctgttgat ggccggcttc atcgccctga acttcatgcg cagtttcgtc
     1741 gggcgcgcgc cgctcgcggc gatcgccggg cttggcggtg gttttctcac ctggtggctc
     1801 acgtcgaaaa tcccgctgtc cgccggcatc ggcgtcggac tcttcttact caccttgtca
     1861 ctgagtggcc gcggcggctc gggacgaggc gggagcggtc gggtatttcg cgacatcgcg
     1921 cgcggcgggg gactcggcgg tctcggcggt ggattcggtg ggcggggagg cggatttggc
     1981 ggcggtggcg gtggttcctt cggaggcggc ggcgcctcgg gccgctggta gttaggccat
     2041 ggaactcatg cgcatgctcc ggcacctgtg cgctacgcgc gcgggcacgc ggcggcggtt
     2101 caccgacgag gtcgacgccg tgatcgaaac cgcgatccgg gccgcggaat cgcgcacttc
     2161 cgccgaaatc cgcttcgtga tcgagaccgc gctcgacatt cccgagctgt gggccggcgt
     2221 gtcgccacgc gaccgcgctt gccaggtttt ttcgcagttc ggcgtgtggg acaccgaact
     2281 tcgcaacggc gcactcatct atgtattggc ggcggatcgc gacgtggaaa tcgtcgtcga
     2341 ccgcggtgct ttcaggattt ccccggtcga gtgggaagcg gcgtgcaaac tgatggaagg
     2401 ccatttccgc gccggccgct tccaggaagg cgctgtcgcg ggagtcgagg ccgtgggcgg
     2461 attgttagcg cgcgagtttc cggtccgctc cggcgatcgt gacgagctcc cgaaccagcc
     2521 ttcgctcctg tagcagaatg ccgacagccc gaaacttccc tgcggtgtca taatttgctc
     2581 atgaaaaccc atgtcgcgct gcgacctctc gttgtcccgg cgctcgtctt cgcgctgctg
     2641 tccggctgct cattactcgg tgaaggtccg aaatggggag cgaagcgtga tgccgacaag
     2701 gccgcgaacg cggtgccgca gattccgctg gccgtgccca cgcaccgctt cgagatcgac
     2761 gagac
//
LOCUS       DATDEU010000055         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1468405, whole genome shotgun sequence.
ACCESSION   DATDEU010000055 DATDEU010000000
VERSION     DATDEU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468405"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VIV63_01210"
     CDS             <1..310
                     /locus_tag="VIV63_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6123239.1"
                     /translation="GREALNALARDSFDLVLMDCNMPDMDGLEATRFLRAGASGVRDV
                     NVPVIALTANAMDGDRERCLAAGMNDFLAKPVTIAALGAAIDRARDARKATSRMKTAV
                     "
     gene            complement(327..1313)
                     /gene="mgrA"
                     /locus_tag="VIV63_01215"
     CDS             complement(327..1313)
                     /gene="mgrA"
                     /locus_tag="VIV63_01215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEY6123240.1"
                     /translation="MGYEPSDARYQSMKYNRVGRSGVRLPAVSLGLWHNFGDVDVFQN
                     GRTIARRAFDLGITHFDLANNYGPSPGSAEETFGALIAKDFHPYRDEMIISSKAGYLM
                     WPGPYGEWGSRKYLVASCDQSLARMGLKYVDIFYSHRFDPETPLEETMGALDYIVRSG
                     RALYAGISSYNPEQTARAAAILKDLGTPCLIHQPSYSMFNRWIEDGLLAELDKTGMGC
                     IVFSPLAQGLLSSKYLNGIPENSRASKHTSLDPDAITPAKVEKVKKLNEIAKSRGQSL
                     AQMAVAWTLRHKQVTSSLIGASKVSHVEEAVGALGNLAFSASELAAIESILK"
     gene            complement(1331..3121)
                     /locus_tag="VIV63_01220"
     CDS             complement(1331..3121)
                     /locus_tag="VIV63_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEY6123241.1"
                     /translation="MKAHKLSWKIYALVLAPVAVLLPAAIVYGHPDALGTRFVAPATG
                     ADTGDCNNNHNPCRTLAYALTQVQPGNAVKLAAGNYDVSGIDVENLLVGKEGVRGGYS
                     AEDHFAIQNAETNPTRMSGVPDAFRNNFIAHGFIVVDANGNPLPRIILPKILVPTACT
                     SGRAGTFPCHNIDYLAQVQLTDIPGAPTSASEIWGIVDMDDQREYAILGHRNGTAFYD
                     VTNPATPVLVANIPGNASLWREVKAYQVYDAALGRHRAYAYATTEAPGGGLQVFDLTN
                     LPDTVTLANTLTQFSTSHTLYISNINYATNAALPGATPYLVIAGANVGGGAFRLYDLT
                     NPVSPTLVTAPPTGTGYMHDSTSMLITDNRTTQCANAHNPCQVLVDFNETSVDLWDVT
                     EKAAPVRLSSTTYPTATYVHSGWPTADQMHIVVHDELDELQRGLYTHIYTLDVGDLRA
                     PSLVTSHIGGTTSTDHNGYAIGNRYYVSHYKRGLVIFDVTNPRALTEVGSFDTYLSPT
                     ANSAGTDGAWGVYPFLTSGTLLVSDIENGMFLLRRNETLPPPVVVTPPSTAPIGGGGG
                     GGGGGGALDLGVLILLAGFAMLRARRQTPA"
BASE COUNT          533 a          985 c         1105 g          608 t
ORIGIN      
        1 tggccgcgaa gcgctcaacg cgctcgcccg cgattcgttc gacctcgtgc tcatggattg
       61 caacatgccg gatatggatg ggctcgaagc cacacggttc ctgcgcgcgg gcgcttccgg
      121 cgtgcgtgac gtgaacgtgc cggtgatcgc gctgacggcc aatgccatgg acggggaccg
      181 cgagcgctgc ctcgccgccg gcatgaatga cttcctggcg aagcccgtga cgattgccgc
      241 gcttggggcc gcgatcgatc gggcgcgcga tgcccgaaaa gccacgtcgc gcatgaagac
      301 cgccgtctaa acggtgcctg gtacggttat ttcagaatcg actcgatagc agcgagctcg
      361 ctcgccgaga acgcgaggtt gcccagcgcg ccgacggcct cttccacgtg cgacaccttg
      421 ctggcgccga tcagcgatga cgtcacctgt ttgtgccgca gcgtccaggc caccgccatc
      481 tgcgccagcg actgaccgcg cgatttcgca atctcgttga gcttcttcac cttctctacc
      541 ttggccggcg tgatggcgtc cgggtcgagt gacgtgtgct tcgaggcgcg tgaattctcg
      601 gggatgccgt tcaggtactt gctgctcagc agcccttgcg ccagcggcga gaacacgatg
      661 cagcccattc ccgtcttgtc gagttcggcg agcaggccgt cttcgatcca gcggttgaac
      721 atgctgtagc tgggctgatg gatgagacac ggcgtaccga ggtccttgag gatcgccgcg
      781 gcccgcgctg tctgctccgg gttgtagctg gagatgccgg cgtacaacgc gcggccggag
      841 cgaacgatgt agtcgagcgc ccccatggtt tcttcgagcg gcgtctccgg atcgaagcgg
      901 tggctgtaga agatgtcgac gtacttgaga cccatgcgcg ccagcgactg gtcgcagctc
      961 gccaccaggt acttgcgcga gccccactcg ccgtacgggc ccggccacat gagatagccg
     1021 gccttcgacg agatgatcat ctcgtcgcgg taggggtgga aatccttagc gatgagcgcg
     1081 ccgaaggttt cttccgccga gccgggcgac gggccgtagt tgttagccag gtcgaaatgc
     1141 gtgatgccga gatcgaacgc acggcgcgcg atggtgcggc cgttctggaa cacgtcgacg
     1201 tcgccgaagt tgtgccagag cccgagcgac accgccggca ggcgcacgcc gctgcgcccc
     1261 acgcggttgt atttcatcga ctggtaacgc gcgtcggacg gctcatatcc catggaacgg
     1321 tctctctttc ttatgcaggg gtttgtcggc gggcgcgtaa catcgcgaac cccgccaaca
     1381 aaatcaagac acccaggtcg agggcaccgc caccgccacc gcctccaccc ccaccaccga
     1441 tgggcgccgt cgaaggcggc gtcacgacca cgggcggtgg cagcgtctcg ttcctgcgca
     1501 gcaggaacat gccgttttcg atgtcgctga ccagaagcgt gcccgacgtg aggaatggat
     1561 agacgcccca cgcgccgtcc gtgcccgcag agttcgcggt gggcgacagg taggtgtcga
     1621 atgacccgac ctccgtgagc gcccgcgggt tcgtcacgtc gaagatcacg agaccacgct
     1681 tgtagtgcga gacgtagtag cgattgccga tcgcgtagcc gttgtggtcc gtgctcgtcg
     1741 ttccaccgat atgcgaagtg acgaggctcg gcgcgcgcaa gtcgccgacg tcgagcgtgt
     1801 agatatgggt gtagagcccg cgctgcagtt catcgagctc gtcgtgaacc acgatgtgca
     1861 tctggtcggc ggtgggccag cccgagtgca cgtaggtcgc ggtcggatat gtcgtggagc
     1921 tcagacgcac gggcgcggcc ttctccgtga cgtcccagag gtcgacggac gtctcgttga
     1981 agtccaccag cacctggcag gggttgtggg cattcgcgca ctgggtagtt cgattgtcgg
     2041 tgatcagcat cgacgtggag tcgtgcatgt atcccgtgcc cgtgggtggc gccgtgacca
     2101 gcgtgggcga cacggggttg gtcaggtcgt agagccggaa cgcgccgccg cccacgttgg
     2161 cgccggcaat cacgaggtag ggcgtggcgc ctggcaacgc ggcgttggtc gcgtagttga
     2221 tgttggagat gtacagcgtg tgcgacgtgc tgaactgcgt cagcgtgttg gccagcgtca
     2281 cggtgtcagg caggttggtg aggtcgaaca cctgcagtcc gccgcccggt gcttccgtgg
     2341 tggcgtaggc ataggcgcga tggcggccga gcgcagcgtc atagacctgg tacgccttca
     2401 cttcgcgcca cagcgacgcg ttgcccggaa tgttggcgac gagcaccggc gtggccgggt
     2461 tggtcacgtc ataaaacgct gtgccattgc gatgcccgag gatcgcgtat tcgcgctggt
     2521 catccatgtc gacgatgccc cagatctcac tcgcgctcgt gggcgcgccg ggaatgtcgg
     2581 tgagctgtac ctgcgcgagg tagtcgatgt tgtggcacgg gaaggtgccc gcccggccgc
     2641 tggtgcaggc ggtgggcacc aggatcttgg gcaggatgat gcgcggcagc gggttgccgt
     2701 tggcgtccac gacgatgaag ccatgcgcga tgaagttgtt gcggaatgcg tccggcacgc
     2761 ccgacatgcg cgtgggattg gtctcggcgt tctgaatggc gaagtgatct tcggcggagt
     2821 aaccgccgcg cacgccttcc ttgcccacga ggagattttc gacgtcgatg ccggagacgt
     2881 cgtagttgcc ggccgccagc ttgacggcgt tacccggttg aacctgcgtg agcgcataag
     2941 ccagcgtgcg gcagggattg tggttgttgt tgcagtcgcc ggtgtcggcg cccgtggcgg
     3001 gtgctacgaa ccgcgtgccc agtgcatcgg ggtggccata aacgatcgcc gccggcagca
     3061 gcaccgcgac cggcgccaac accagggcat agatcttcca ggacaatttg tgagctttca
     3121 tgggccctcc ttgcgagcaa ttccggacga agattagtgc cccgctgggg ctcgttagtt
     3181 ctcggcgctc gtcacgttct cagcgcgtga tcagccagac acccgcgccg a
//
LOCUS       DATDEU010000056        11672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_884696, whole genome shotgun sequence.
ACCESSION   DATDEU010000056 DATDEU010000000
VERSION     DATDEU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884696"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VIV63_01225"
     CDS             <1..349
                     /locus_tag="VIV63_01225"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY6123242.1"
                     /translation="DSPNRHWKNRDLAPRDRLSSVFYAVTAHDEVQPTHLHVRDRNIC
                     ATTCATEYGNPCTRFCPAAVYEMVDDDNGGKKLHINAANCVHCKACDIKDPYQIIDWV
                     TPEGGSGPNYQLL"
     gene            346..1452
                     /gene="zapE"
                     /locus_tag="VIV63_01230"
     CDS             346..1452
                     /gene="zapE"
                     /locus_tag="VIV63_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY6123243.1"
                     /translation="MTETQDALGAAYAGEIAKRGFAADSAQLAAVRHLERLRRELAEA
                     ASAPLGKRLLRGLTSSATAPKGVYLWGGVGRGKTWLMDLFYASLSQTTKRRTHFYRFM
                     QDVHADLKRLKGKQSPLDGVADKIAKKAHVICFDELFVSDIADAMILGTLFDALIKRG
                     VALVFTSNVRPKDLYKGGLQRERFLPTIALLEKHCEVVAVDGGVDYRLRQLTAAPIYL
                     PAAGADTPKKLEELFDDLSDDDVESGGTITVNHRKIKVVRESENVIWFDFAALCEGPR
                     SPADYVAIASEYQSVIIAGVPVFDDTSDNAARRFISVIDEFYDRGVNVILSAAAAPAE
                     LYRGEKLKFEFQRTASRLVEMQSTDYLSRAHRAA"
     gene            complement(1459..2529)
                     /locus_tag="VIV63_01235"
     CDS             complement(1459..2529)
                     /locus_tag="VIV63_01235"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY6123244.1"
                     /translation="MWKNTEVTRRLKLDLPIVQGPFGSGLSAVDLVVAVSEGGGLGSF
                     GVHHLDGAGIRSIDAQIRARTRRTYALNLWVPLGDSDDPRMTGAQWEAAVGLLRPYFT
                     ELGVPLPERPARFGPYYQEQIETLLELRPPVFSFVFGVPAPDVLDRCRSAGIATLGAA
                     TTAAEAKLLADAGVDMIVASGFEAGGHRVSFLQEPEDCLTGTMALIPQVADAVRVPVI
                     AAGGIADGRGIAAALKLGAQAVQVGTAFLACEESNATPLHRTKLFSEDARRTTLTRAF
                     TGRLARSVHNGFIDALRGQEKVLAPYPVQAWLTAQFKSAALAANRSDLMSLWSGQGAP
                     LLKHRRAGELLRALVREADEAQ"
     gene            2591..2956
                     /locus_tag="VIV63_01240"
     CDS             2591..2956
                     /locus_tag="VIV63_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6123245.1"
                     /translation="MPKANPFNPDCPSQEILALIGSKWSLLLLCILRNGPARPGELAR
                     RTGGISQKMLTQTLRELERHGIVARRDYGTVPPRVEYSLTPLGESLGGLVQQIERWLE
                     SNYPRLARAARSYDANAPA"
     gene            complement(2959..3846)
                     /locus_tag="VIV63_01245"
     CDS             complement(2959..3846)
                     /locus_tag="VIV63_01245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6123246.1"
                     /translation="MNTASDVMESAPGVRARAVDAWTAFWADADQSRCAAGAPEIGLA
                     LTNHWSNFARSLAPGTPILDLGCGACAVGRLVVGASADLHVTGIDAATTPRTPHPRID
                     VLSHTAMEALPFTGRRFGAVVSQFGYEYSRIDVTAREVARVVTPGARLSLLVHHDESA
                     IVASTRARLDAIERLLGASLRAAFCAGDAAGFHAQMTALIALHPHDDLVACLARALQP
                     RVASPQAKRIATWSAIEEALAPERCVSEALCECCVPALRLDEWLEPLRATCRLLPVSV
                     LREPNGAAIAWKIEGVYAG"
     gene            complement(4076..4711)
                     /locus_tag="VIV63_01250"
     CDS             complement(4076..4711)
                     /locus_tag="VIV63_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY6123247.1"
                     /translation="MPWTREDLAKRAARELRDGYYVNLGIGIPTLVANYIPPGIDVVL
                     QSENGMLGMGPFPYEGDEDPDLINAGKQTITELATSSFFSSADSFGMIRGGHINLSVL
                     GALEVAENGDLANWMVPGKMVKGMGGAMDLVAGVRRVVVVMEHNSKDGAAKFKPRCDL
                     PLTGANCVDMLITDLAVFERPDRKSKFRLIELAPGVTEAQVRDKTSAKFEA"
     gene            complement(4711..5424)
                     /locus_tag="VIV63_01255"
     CDS             complement(4711..5424)
                     /locus_tag="VIV63_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY6123248.1"
                     /translation="MPTSKVHADAKSALAGLTRDGMTVMSGGFGLCGIPENLIGALRD
                     SGARDLTVISNNAGVDGFGLGLLLETRQIRKMVSSYVGENKEFERQYLARELELEFNP
                     QGTLAERIRAGGAGIYGFYTRTGAGTVVAEGKEQRDVNGELFVLETGLTADLAIVKAW
                     KGDTEGNLVYRKTARNFNPMMATAGRVTVAEVEELVEPGVLDPDQIHTPGIFVQRIIR
                     GTNYQKRIEQRTVRKRGAA"
     gene            complement(5414..6064)
                     /gene="maiA"
                     /locus_tag="VIV63_01260"
     CDS             complement(5414..6064)
                     /gene="maiA"
                     /locus_tag="VIV63_01260"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY6123249.1"
                     /translation="MSLELYSYWRSSASYRVRIALNLKSLPYVTHGVNLMRDGGEQWS
                     VKYRDVNPQSRVPTLVHDGQRFTQSLAIIEYLDETFPDHRLIPRDPVDRARVRMLSQI
                     IACDVQPLQNTSTTLYLKDKLKLDEAAITAWLQEWIVRGLGAFNAHLERDHLSGKFCH
                     GDTPTMADCCLVPQLFAASRFGVEVTRYPRLALIAENCAALSAFQHAHPSKQPDAN"
     gene            complement(6061..7107)
                     /gene="hppD"
                     /locus_tag="VIV63_01265"
     CDS             complement(6061..7107)
                     /gene="hppD"
                     /locus_tag="VIV63_01265"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY6123250.1"
                     /translation="MADFINPMGTDGFEFVEYAAPDPQLLRSLFERLGFPVVARHRSK
                     NVTLHSQGDINFVINAEPNSFGQQFARLHGPSVCAMAFRVNDAAAAFERAISLGAEPH
                     RGSVGPMELNIPAIVGIGGSLIYFVDRYGEHSIYDVDFKPVAAQPLERAGLLHIDHLT
                     HNVHRGNMDKWTAFYETLFNFREIRYFDIEGKKTGLFSRALTSPCGKIRIPINESQDD
                     KSQIEEYLREYRGEGIQHIALSTEDIYRTVDVLRGRTVAFQDTPETYYEGIAGRVTGH
                     QENVEELRKRRILIDGSGANDEGILLQIFTANVIGPIFFEIIQRKGNQGFGEGNFKAL
                     FESIELDQIRRGVI"
     gene            7184..7648
                     /locus_tag="VIV63_01270"
     CDS             7184..7648
                     /locus_tag="VIV63_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY6123251.1"
                     /translation="MTRREKLHLEGFLPYRLSVLSNTVSSAIAAAYFANFGLSIPEWR
                     VMAVLAANPGLVAAEVTARTAMDKVAVSRAVATLLDAGRLQRTTAPSDRRRTHLALTT
                     AGARVYAQVVPMALKYERQLVAPLSKSDLATLDRILRVLLGRAVELGPAHLQ"
     gene            7725..>11672
                     /locus_tag="VIV63_01275"
     CDS             7725..>11672
                     /locus_tag="VIV63_01275"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEY6123252.1"
                     /translation="MLGKIPATRLALIEKIVTHALKGTPRARHALTREFLGAFFRGVA
                     EEDLRAHRPADLAAAALSHLEFGRRRTGHEVLVDIAPSLNLDAPTASHRALLRVVSPD
                     MPFLVDSIGIVFSQMNIAVHLIVHPVLAVRRDSRGTIRMVGVDRRDAQLESWQMMEID
                     RPRDAEAASELEKRIRAALDDVRRAVADFPAMLERARAVANQLDRTPLPVPKSHASEA
                     RALLAWMHDGHFVFLGYRHYQLKRGRSRDALVRDSASGLGILREPAARKGSRKASPPI
                     VLTGHLRQQARAPELLVLTKANTASTVHRGSYLDYVGVKTFDASGQASGEHRFLGLWT
                     SSAYHKPPAEIPLLRRKLEAVIAHFGLPAQSHDAKSVVNVIETFPRDELFQTPLPELI
                     ASVRGIVNLYERRRVRLFVRRDSFERFYSCLVYVPRDRYNTEVRERIERIVRQRFGGT
                     HVETQVQISDSMLARLHMLVRTPQGTPGVEDIPAIEAEIAAASATWEDRLQQALISRG
                     VERSAVELAARYARTFPPAYRADVEPAQALEDIADLEALAADPSTPQINLRERRGGRL
                     SLRILQVSEPISISDILPMLENFGLRVLAEHPYQVVAAERTVWIQDFELEARDLKRAD
                     IAALEPLFKEAFLAAWRGDIENDGFNRLLLCASLGARDIVVVRAYCRYLLQTGIPFSQ
                     AYMERVLVAQAPITGALVRLFQTQFALGNKARESTAERIAKGILRALDKVASLDEDRI
                     LRSYLHVIRATLRTNYYQSADGSTAGKPKSWVSFKLDPHAIPDLPLPRPRFEIFVYSP
                     RVEGVHMRMGYVARGGLRWSDRREDFRTEVLGLMKAQHVKNTVIVPVGAKGGFYPKRL
                     PAGGSRDDVQKEVVASYQTFIRGLLDVTDNIVNGKVVVRPGIVRRDGDDAYLVVAADK
                     GTATFSDIANAISIEYGHWLGDAFASGGSAGYDHKGMGITARGAWECVKRHFRELGVD
                     IQSQEFTCAGVGDMSGDVFGNGMLLSKQTRLVAAFDHRHIFIDPTPQAAASFRERERL
                     FKLPRSSWDDYSRKLISRGGGVWPRSAKSIPLSAETQKVLDLAVPAATPVEVMRAILR
                     MRVDLLWNGGIGTYVKSTDESHADVRDRANDAIRADGHEIRARVVGEGGNLGCTQRGR
                     VEYSQAGGRINTDFIDNSAGVNTSDVEVNIKILLSEVERRGRLPLAARNKLLASMTDE
                     VAGLVLRNNYLQTQALSVLEQRAPERLAEYQSLIRELERNGNLDRAIEFLPGDDEFLE
                     RRKQRLGLTRPELAVVLAYSKIWLSNHLLDSDLPDDPYFASEVQRYFPAPMRKR"
BASE COUNT         2042 a         4018 c         3628 g         1984 t
ORIGIN      
        1 cgactcgccg aaccggcact ggaaaaatcg cgacctcgcg ccgcgcgacc ggctgtcgtc
       61 agtgttctac gcggtcaccg cgcacgacga agtgcagccg acccacctgc acgtgcgcga
      121 ccggaacatc tgcgcgacca cctgcgccac ggagtatggc aatccgtgca cgcgcttctg
      181 ccccgctgcg gtttatgaaa tggtggacga tgacaacggc ggcaagaagc tgcacatcaa
      241 cgcggccaac tgcgtgcact gcaaggcctg cgacatcaag gacccgtacc agatcatcga
      301 ctgggtgacg cccgaaggcg gttccggtcc caactaccag ctgctgtgac cgaaacccag
      361 gacgccctcg gcgcagcgta cgccggggaa atagccaaac gcggcttcgc cgccgacagc
      421 gcgcaacttg cggcggtgcg ccacctcgag cgattgcgcc gcgaactcgc cgaagcggcg
      481 tccgcgccgc tcggcaaacg cctgctgcga ggcctcacca gttccgccac ggcaccgaag
      541 ggcgtctatc tctggggcgg cgtaggccgc ggcaagacct ggctcatgga cctcttctac
      601 gccagcctca gccagaccac gaaacgccgc acgcacttct accggttcat gcaggacgtg
      661 catgccgacc tcaagcggct caagggcaaa caatcgccgc tcgacggggt cgccgacaag
      721 atcgccaaga aggcgcacgt catctgtttc gacgagttgt tcgtttcgga catcgctgac
      781 gccatgatcc tcggcacgct gttcgacgcg ttgatcaagc gcggcgtcgc gctggtcttc
      841 acctccaacg tccggcctaa ggatctctac aagggcggcc tgcagcgcga acgcttcctc
      901 ccgaccatcg cgttgctgga gaaacattgc gaagtggtcg ccgtcgacgg cggtgtcgat
      961 taccgcctgc gccagctcac ggccgctccc atctacttgc ccgccgcggg cgccgacacg
     1021 ccgaagaagc tcgaagagct gttcgatgac ctgtcggatg acgacgtcga atcgggcggc
     1081 acgattaccg tcaaccaccg caagatcaag gtggttcgcg aaagcgagaa cgtcatctgg
     1141 ttcgattttg cggcgctgtg cgaaggtccg cgcagtccgg cggactacgt ggccatcgcc
     1201 agcgaatacc agtcggtgat catcgccggc gtgcccgtgt tcgacgacac ctccgacaac
     1261 gccgcgcggc gtttcatttc ggtgatcgac gaattctacg atcgcggcgt caacgtcatt
     1321 ctttccgccg ccgctgcgcc ggccgagttg tatcgcggcg agaaactcaa gttcgagttc
     1381 cagcggaccg ccagccgcct cgtcgagatg cagagcacgg actacctgtc acgtgcccat
     1441 cgcgcggcgt aattcctctt actgagcctc atcggcttcg cgtaccaatg cgcgcagcaa
     1501 ctcgcccgcg cggcgatgct tcagcaacgg cgcgccctgc cccgaccaca gcgacatcag
     1561 atcggatcga ttcgcggcca gcgccgccga cttgaattgc gccgtgagcc aagcctgaac
     1621 cgggtatggc gcgagcacct tctcctgtcc ccgtaacgcg tcgatgaagc cgttgtgaac
     1681 gctgcgcgcg agacggccgg tgaacgcgcg cgtcaacgtc gtgcgacgcg catcctcgct
     1741 gaacagtttc gtccgatgca gcggcgtggc attcgactcc tcgcaggcca ggaacgccgt
     1801 gcccacctgc acggcctgcg cgcccagctt cagggcggcc gcgatcccgc gcccatcggc
     1861 gatgccaccc gcggcaatga ccggcacccg gaccgcgtcg gccacctgcg gaatcagcgc
     1921 catcgtcccc gtcaggcagt cctcgggctc ctggagaaac gacacccggt gcccgcccgc
     1981 ctcgaatccc gacgcgacga tcatgtcgac gccggcatcg gccagtagtt tggcctccgc
     2041 ggccgtggtc gccgcgccca gcgtggcgat gccggcgctt cggcaacggt cgagaacatc
     2101 cggcgccggg accccgaaga cgaagctgaa caccggcggc cgcagttcga gcagcgtctc
     2161 gatctgctcc tggtagtaag gcccgaaacg cgccggccgc tccggcaacg gcactccgag
     2221 ttcggtgaag tacgggcgca gcagcccgac cgcggcctcc cattgcgcac cggtcatgcg
     2281 gggatcgtcg ctgtcaccaa gcggaaccca caggttcaac gcgtatgttc tgcgcgtgcg
     2341 cgcgcgaatc tgcgcgtcga tcgaacggat ccccgcgccg tcgaggtgat gcacaccgaa
     2401 agacccgagc ccgccgcctt cactgacggc caccacgagg tccaccgcgg acagtccgct
     2461 gccgaacggt ccctgcacga tgggcaggtc gagtttcagt cttcgcgtga cttcagtgtt
     2521 cttccacatg ccgcgaagag taagctcatg cgctccatct gataagtacg cacctttcgg
     2581 tgcctgcccc atgcccaaag cgaatccgtt caatcccgac tgcccttcgc aggaaatcct
     2641 cgcgctcatc ggcagcaagt ggtccttgtt gctgttgtgc atcctgcgca atgggcctgc
     2701 ccgccccggc gagctcgcgc ggcgcaccgg tggcatttcg cagaagatgc tgacgcagac
     2761 gctgcgggag ctggagcgcc acggcatcgt cgcccggcgc gactacggca cggttccgcc
     2821 gcgagtcgaa tattccctca cgccgctcgg cgagtcgctg ggtggcctgg tccagcagat
     2881 cgagcgttgg ttggagtcca actacccgcg cctggcgcgc gccgcgcgtt cgtacgacgc
     2941 gaacgccccg gcctgacgtc aaccggcgta cacgccttcg attttccacg cgatggcagc
     3001 tccgttcggc tcccgcagga cggagaccgg cagcagccga caggttgcgc gcaatggctc
     3061 taaccactcg tccaggcgca gggccggcac acaacactcg cacagggcct ccgatacgca
     3121 tcgttcgggc gccagggctt cctcgatggc cgaccaggtc gcgatccttt ttgcctgcgg
     3181 gctcgccacg cgcggctgca gcgcccgcgc gagacaggcg acgaggtcgt cgtgtggatg
     3241 cagggcaatc agtgccgtca tctgcgcatg aaagccggcc gcgtccccgg cgcagaatgc
     3301 cgcgcgcagt gacgcgccga gaagccgctc gatggcatcc aggcgcgctc gcgtcgacgc
     3361 gacgatcgcg ctttcatcgt ggtgtaccag cagcgacagc ctggcgcccg gcgtgacaac
     3421 ccgcgccact tcgcgcgcgg tcacatcgat ccggctgtat tcgtacccga actgactgac
     3481 cactgcgccg aaccgtcggc ctgtgaacgg caacgcctcc atcgcggtgt gcgacaacac
     3541 gtcgatccgc ggatggggtg tgcgcggcgt cgtcgccgcg tcgatgcccg tgacatgcag
     3601 gtccgcgctt gcgccgacca ccagccgccc caccgcacag gcgccgcagc ccagatcgag
     3661 gatcggcgtg cccggcgcga gagatctggc gaagttcgac cagtggttcg tcagcgccag
     3721 accaatttcg ggcgcacccg cggcgcaacg ggactgatcg gcgtccgccc agaacgcggt
     3781 ccatgcgtct acggcacgag cgcgaacgcc cggcgcgctc tccatgacat ctgatgcggt
     3841 attcatccca ttcacacaga cgcaacggcg gaagggaagc ggacaccgct accgcgcggc
     3901 tgcacctatc cattggtttg cctggcgata tcttgcgcgt cggagtttca ggatcgacgc
     3961 gcgcgcgcct ccgcctggcg tcggcagagc tcgattcgcc gcgtccgaaa acgattgaaa
     4021 atgaagtcgc ttggccgcgg gtcaggacga gcccgcgacg ggacgaaccg agtggttagg
     4081 cctcgaactt cgccgacgtc ttgtcccgaa cctgggcctc ggtgacgccg ggagcaagct
     4141 cgatgagcct gaacttcgac ttgcggtccg gccgctcgaa caccgccaga tccgtgatca
     4201 gcatgtcgac gcagttcgcg cccgtcagcg gcagatcaca acgcggcttg aacttcgccg
     4261 caccgtcttt cgagttgtgc tccatcacca ccaccacgcg gcgcacgccg gcgacgaggt
     4321 ccatggcgcc acccatgcct ttcaccatct tgccgggcac catccagttg gcgaggtcgc
     4381 cgttctcggc gacttcgagc gcgccgagaa ccgagaggtt gatgtgcccg ccgcgaatca
     4441 tgccgaagct gtccgccgat gaaaaaaacg acgacgtcgc gagctcggtg atggtctgct
     4501 tgccggcgtt gatgaggtcg ggatcttcat cgccttcata cggaaacggc cccatgccca
     4561 gcatgccgtt ctccgactgc agcaccacgt cgatgccggg cggaatgtag ttggccacca
     4621 gcgtcggaat gccgatgccg aggttgacgt agtagccgtc gcgcagctcg cgggcggcgc
     4681 gcttcgccag gtcttcgcgc gtccacggca tcaggccgct ccccgtttgc gcacggtccg
     4741 ctgttcgatg cgtttctggt agttcgttcc gcggatgatc cgctgcacga aaattccggg
     4801 cgtgtggatc tggtcggggt cgagcacacc gggctccacc agctcttcga cctcggccac
     4861 ggtcacacgc cccgcggtcg ccatcatcgg attgaaattc cgcgccgtct tgcgatacac
     4921 gagattgccc tcggtatcac ccttccaggc tttcacgatc gcaagatccg cggtgagtcc
     4981 cgtctccagc acgaacaact caccgttgac gtcacgctgc tccttgcctt cggccaccac
     5041 cgtgccagcg ccggtgcgcg tatagaaacc gtagatgccg gcgccgccgg cgcgaattct
     5101 ctccgccagc gtgccctgcg gattgaactc gagttcgagt tccctggcca ggtactggcg
     5161 ttcgaattcc ttgttctcgc ccacgtacga cgaaaccatc ttgcggatct gccgcgtctc
     5221 gagcagcaac cccaggccga agccgtcgac gcccgcgttg ttggagatca ccgtgagatc
     5281 gcgcgcaccc gagtcgcgca gcgcgccgat gagattttcg gggatgccgc acaggccgaa
     5341 gccgcccgac atcacggtca ttccgtcgcg cgtgagaccc gcgagcgcgg acttcgcgtc
     5401 agcgtgaacc ttgctagttg gcatcaggct gtttcgaagg atgagcgtgc tggaaagccg
     5461 acagcgccgc gcagttctcc gcgatcagcg ccaggcgcgg gtaccgcgtg acctcgacgc
     5521 cgaaacgcga agcggcgaac aactgcggca cgaggcagca atccgccatg gtcggcgtat
     5581 cgccgtggca gaacttgccg ctcaggtgat cgcgctcgag gtgcgcgttg aacgcgccga
     5641 gcccgcgcac gatccattcc tgcagccacg cggtgatggc cgcctcgtcg agtttcagct
     5701 tgtccttgag atagagcgtg gtgctggtgt tctgcagcgg ctgcacgtcg caggcgatga
     5761 tctgcgagag catgcgcacg cgggcgcgat cgaccggatc gcgcggaatc aggcgatgat
     5821 cgggaaaggt ttcgtcgagg tattcgatga tcgccagcga ctgcgtgaaa cgctggccgt
     5881 cgtgcaccag cgtgggcaca cggctctggg gattcacgtc gcgatacttg acggaccact
     5941 gctcgccgcc gtcgcgcatc aggttaacgc cgtgcgtgac gtatggcagg gacttgagat
     6001 tgagcgcgat gcgcacgcga taggacgcgg agctgcgcca gtagctgtag agctcgagac
     6061 tcaaatgaca ccccggcgaa tctggtcgag ttcgatggac tcgaacaatg ccttgaaatt
     6121 cccttcgccg aaaccctggt ttcccttgcg ctggatgatt tcgaagaaga tcggcccgat
     6181 gacattcgcg gtgaagatct gcagcaggat gccttcgtcg ttggcgccac tgccgtcgat
     6241 gaggatgcgg cgcttgcgca gctcctcgac attctcctga tggccggtca cgcggccggc
     6301 gatcccctcg tagtaggtct cgggcgtgtc ctggaaggcc acggtgcggc cgcgcaatac
     6361 atccacggtg cggtagatgt cttcggtgga tagcgcgatg tgctggattc cctcgccgcg
     6421 atattcgcgc aggtattcct cgatctgcga cttgtcgtcc tgcgattcgt tgatcggaat
     6481 gcggatcttg ccgcaggggc tggtcaacgc gcgggagaac aatccggtct tcttgccttc
     6541 gatgtcgaaa tagcggatct cgcggaagtt gaacagcgtc tcgtagaaag ccgtccactt
     6601 gtccatgttg ccgcgatgca cgttgtgcgt gagatggtcg atgtgcaaca gcccggcgcg
     6661 ttccagcggc tgcgcagcca ccggcttgaa gtccacgtcg tagatcgagt gctcgccgta
     6721 ccggtcgacg aaatagatga gcgagccgcc gatgccgacg atggccggga tgttgagctc
     6781 catgggcccg acactgccgc gatgcggctc ggcaccgagc gagatggcgc gctcgaaggc
     6841 cgcggcggcg tcgttgacgc gaaacgccat ggcgcacacc gaaggtccgt gcaggcgcgc
     6901 gaactgctgg ccgaaggaat tcggttccgc gttgatgacg aaattgatgt cgccctggga
     6961 atgcagggtc acgttcttgg agcggtgacg ggcgaccacg gggaacccca gccgttcgaa
     7021 caggctgcgc agcaactggg gatcgggcgc ggcgtattcg acgaactcga agccgtcggt
     7081 gcccattggg ttgatgaagt cagccatcac tggcctccga ccggaaattg atttagtttc
     7141 aattgtaacc attccgccgg aatgcgaaag gcgcaagcag cccatgacgc gacgcgagaa
     7201 gctccacctg gaaggatttc ttccctaccg gctgtcggtg ttgtccaata ccgtgagttc
     7261 cgcgattgcg gccgcctatt tcgccaactt cggactttcg atccccgaat ggcgcgtcat
     7321 ggcggtcctg gccgccaatc ccggccttgt cgcagcggag gtcaccgcgc gcaccgccat
     7381 ggacaaggtc gccgtgagcc gggccgtggc caccttgctc gacgccggcc gcttgcagcg
     7441 caccacggcg ccctctgacc ggcgacgcac gcacctcgca ttgaccacgg ccggcgcacg
     7501 cgtgtacgcg caggtcgtgc ccatggcgct gaaatacgaa cgtcagctcg tggccccgct
     7561 gtcgaagagc gacctcgcca ccctcgaccg gattctgcgg gttttgctag gccgcgccgt
     7621 cgaactcggt cccgcccacc tgcagtgagc ccctgcggcc gtatgtataa tcgcgcgcgc
     7681 tgatccgcgc gcctctcacc ccttcccctt tccagagagt cccaatgctc ggcaagattc
     7741 ccgcgacgcg cctcgcgctc atcgagaaga tagtcacgca cgccctcaag ggcacgccgc
     7801 gcgcgcgtca tgcgctgacg cgcgaattcc tcggcgcgtt cttcagaggc gtcgccgaag
     7861 aagatcttcg cgcccatcgc ccggcggacc tggcggccgc ggcgctctcc catctcgaat
     7921 tcggacggcg gcgaaccggc cacgaagtgc tggtcgacat cgcaccgtcg ctcaacctcg
     7981 atgcgcccac cgcctcgcat cgcgcgctgt tgcgcgtggt gtcgccggac atgccctttc
     8041 tcgtcgattc catcggcatc gtcttcagcc agatgaacat cgccgttcac ctcatcgttc
     8101 atcccgtact cgccgtgcgc cgcgactcgc gcggcaccat caggatggtc ggcgtcgacc
     8161 gccgcgacgc gcagctcgaa tcctggcaga tgatggagat agatcgtccg cgcgacgccg
     8221 aagccgccag cgaactcgaa aagcgcatcc gtgccgcgct cgacgacgtg cgccgcgcgg
     8281 tggccgattt tcccgccatg ctcgaacgcg cccgcgccgt cgccaaccaa ctcgatcgca
     8341 cgccgctgcc cgtacccaag tcacacgcca gcgaggcgcg cgccctgctc gcctggatgc
     8401 acgacggtca tttcgtattc ctcggttatc gccactacca gttgaagcgc ggccggtcgc
     8461 gcgacgcgct ggtgcgcgac agcgccagtg gcctcggaat cctgcgcgaa cccgccgcgc
     8521 gcaaagggtc gcgcaaggca tcgccgccca tcgtgctcac gggccatctg cgccagcagg
     8581 cgcgcgcgcc cgaactgctc gtgctcacca aggccaacac cgcatccacc gtgcatcgtg
     8641 gcagctacct ggactacgtc ggggtcaaga cgttcgacgc ttccgggcag gcgtccggcg
     8701 aacatcgttt cctcggcctc tggacctcga gcgcctatca caagccgccc gcggaaattc
     8761 cgctgcttcg ccgcaagctc gaagcggtca tcgcgcactt cggcctgccc gcgcagagcc
     8821 acgacgccaa gtccgtggtc aacgtgatcg aaacatttcc gcgcgacgaa ctgttccaga
     8881 cgcccttgcc ggaactcatc gcgagcgtac gcggcatcgt caatctctac gaacggcgcc
     8941 gcgtgcgcct gttcgtgcgc cgtgacagct tcgagcgttt ctactcctgc ctcgtctacg
     9001 tgccgcgcga ccgctacaac accgaagtcc gcgaacgcat cgagcgcatc gtgcggcagc
     9061 gtttcggcgg cacccacgtc gaaacccagg tccagatctc cgactccatg ctggcgcgcc
     9121 ttcacatgct ggtgcgcacg ccgcagggca cgcccggcgt cgaagacatt cccgccatcg
     9181 aagccgagat cgccgcggcc tccgccacct gggaagaccg actgcagcag gcgctgatct
     9241 cgcgcggcgt ggaacgcagc gcggtggaac tggcggcgcg ctacgccagg acattcccgc
     9301 ccgcctatcg cgccgatgtc gagcccgcac aggcgctcga agacatcgcg gatctcgagg
     9361 cgctggccgc ggacccttcg actccccaga tcaacctgcg cgaaaggcgc ggcggtcgcc
     9421 tgtcgctgcg catcctgcag gtcagcgagc ccatctccat ctccgacatc ctgcccatgc
     9481 tcgagaattt cgggctgcgc gtgctcgccg aacatcccta ccaggtggtc gcggccgagc
     9541 gcacggtttg gatccaggat ttcgaactcg aagcccgcga tctcaagcgc gcggacatcg
     9601 cggcgctgga gccgttgttc aaagaggcct tcctcgccgc ctggcgtggt gacatcgaga
     9661 acgacggatt caatcgcctg ctgctgtgcg cgtcgctggg cgcgcgggac atcgtggtgg
     9721 tgcgcgccta ctgccgctac ctgctgcaga ccggcatccc gttcagccag gcctacatgg
     9781 aacgtgtgct ggtggcgcag gcgccgatca ccggcgccct ggtgcggttg ttccagacgc
     9841 agttcgcgct cggcaacaag gcgcgcgaga gcaccgcgga gcgcatcgcc aagggcatcc
     9901 tgcgcgcgct cgacaaggtc gccagcctcg acgaagaccg tatcctgcgc agctacctgc
     9961 acgtgatccg cgcgacgctg cgcactaact actatcaatc ggcggatgga tcgacggccg
    10021 gcaagccgaa gagctgggtt tcgttcaagc tcgatccgca cgccattccc gacctgccgt
    10081 tgccacggcc ccggttcgaa atcttcgtgt acagcccgcg cgtcgagggt gttcacatgc
    10141 gcatgggcta cgtggcgcgc ggcggcttgc gctggtccga ccgccgcgaa gacttccgca
    10201 ccgaagtgct cgggctcatg aaggcccagc acgtcaagaa cacggtcatc gtgccggtgg
    10261 gcgccaaggg cggcttttat ccgaagcgcc tgcccgcggg cggttcacgc gacgacgtgc
    10321 agaaggaagt cgtcgcgagc taccagactt tcatccgcgg cctgctcgac gtcaccgaca
    10381 acatcgtgaa cggcaaggtc gtcgtgcgcc ccggcatcgt gcgccgcgac ggcgatgacg
    10441 cctacctggt ggtggcggcg gacaagggca cggccacgtt ctccgatatc gccaatgcca
    10501 tctccatcga gtacggccac tggttaggcg acgcgttcgc ctcgggcgga tcggccggct
    10561 acgaccacaa gggcatgggc atcacggcac gcggcgcctg ggagtgcgtg aagcgccact
    10621 tccgtgaact cggtgtcgac atccagtcgc aggaattcac ctgtgccggc gtcggcgaca
    10681 tgtccggcga cgtcttcggc aacggcatgc ttctgtcgaa gcagacgcgc ctggttgcgg
    10741 cattcgatca ccggcacatc ttcatcgacc ccactcccca ggccgcggcg agcttccgcg
    10801 aacgcgagcg actgttcaaa ctaccgcgct cgagctggga cgattactcg cgcaaactca
    10861 tttcgcgcgg tggcggcgtg tggccgcgca gcgccaagtc gattccgctg tccgctgaaa
    10921 cgcaaaaggt gctcgacctc gcggttcccg cggcaacgcc ggtggaggtc atgcgcgcca
    10981 tcctgcgcat gcgcgtcgac cttctgtgga acggcggcat cggcacttac gtgaagtcca
    11041 cggacgagtc gcatgcggat gtccgcgacc gcgccaacga cgccattcgc gccgacggcc
    11101 acgaaatccg cgccagggtg gtcggcgaag gcggcaatct gggctgcacg cagcgtggcc
    11161 gcgtggagta ctcccaggcg ggtgggcgga tcaacaccga cttcatcgac aactccgccg
    11221 gcgtgaacac ttccgacgtc gaagtgaaca tcaagatcct gctctcggaa gtggaacgcc
    11281 gcggcaggct gccgctggcc gcgcgcaaca aactactggc gagcatgacc gacgaggtcg
    11341 ccggcctggt gctgcggaac aactacctgc agacccaggc attgtcggtg ctcgaacagc
    11401 gcgcgcccga acggctggcg gagtaccaga gcctgattcg cgagctcgag cggaatggca
    11461 atctcgatcg cgccatcgaa ttcctgccgg gcgacgacga attcctcgag cgccgcaagc
    11521 agcgcctggg gctcacgcgt ccggagctcg ccgtggtcct cgcctacagc aagatctggc
    11581 tcagcaatca cctgctcgac tccgatctgc ccgacgaccc ctacttcgcg agtgaagtgc
    11641 agcgttattt ccccgcaccg atgcgcaaac gg
//
LOCUS       DATDEU010000057         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1760088, whole genome shotgun sequence.
ACCESSION   DATDEU010000057 DATDEU010000000
VERSION     DATDEU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760088"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /gene="dapC"
                     /locus_tag="VIV63_01280"
     CDS             <1..1220
                     /gene="dapC"
                     /locus_tag="VIV63_01280"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY6123253.1"
                     /translation="TRSPRRRVNQRLELLAAYPFERLARLKAGITPPAGLSHIAMSIG
                     EPKHAPPPFVIEALRRELSKLDSYPVTAGLPETRASFAAWLARRFKVNVSAETMVLPV
                     NGTREALFSFVQAVVSNSATGVRPVVAMPNPFYQIYEGAALLAGAEPQFLNTTAGNRF
                     QPDLDSVPAEIWKRCEILFLCSPGNPTGAVLSLEFLRHALRLADKHDFVIASDECYAE
                     LYRDENAPPPSLLQAALANGHESFQRCMVFHSLSKRSSVPGLRSGFVAGDPALIKPYL
                     LYRTYHGCAMPVPTQLASIAAWDDDAHAAANRVLYREKYARVLPILAPVLDVVEPDGA
                     FYLWPDVQRDDESFTRDLYATQNLTILPGSYLARDTRAGNPGRQRVRISLVAPVEECV
                     VAAQRIRTFLETT"
     gene            1230..2063
                     /gene="dapD"
                     /locus_tag="VIV63_01285"
     CDS             1230..2063
                     /gene="dapD"
                     /locus_tag="VIV63_01285"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY6123254.1"
                     /translation="MTTLAATIDEAFERRAELTAKSAPASVRHAVEESIELLDSGQAR
                     VAEKKDGQWTVNQWLKKAVLLYFRTHDNTIMDAGYTRFFDKVPLKYAARSQADDGDAL
                     RNGGTRVVPPAVARRGSYIAPNTVLMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNV
                     HLSGGVGIGGVLEPLQASPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRI
                     YDRATGQILQGRIPAGSVVVSGNLPSSDGKYSLYCAVIVKRVDAQTRAKTSINELLRA
                     D"
     gene            2089..>2483
                     /locus_tag="VIV63_01290"
     CDS             2089..>2483
                     /locus_tag="VIV63_01290"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6123255.1"
                     /translation="MIAELGLEPHPEGGYYREIFRSARLVTPLDGRGARAALTTIYFL
                     LPGGACSRWHRVTSDEVWHLYEGGPLEVLEIDAGLSQLTRHQLVVASETPVCTIAADC
                     WQAARALADYALVGCTVGPGFDFADFTLLA"
BASE COUNT          425 a          883 c          753 g          422 t
ORIGIN      
        1 gcactcgatc gccgcgccgc cgcgtgaacc agcgcctcga actactcgcc gcctacccct
       61 tcgagcgact ggcgcgactc aaggccggaa tcaccccgcc cgccgggctt tcgcacatcg
      121 ccatgtccat cggcgagccc aaacacgcgc cgccgccatt cgtcatcgag gcgctgcgcc
      181 gcgaactctc gaagctcgat agctacccgg tgaccgcggg tcttccggag acgcgcgcca
      241 gtttcgcggc ctggctggcg aggcgattca aggtgaatgt atccgccgaa accatggtgt
      301 tgccggtcaa cggcacgcgc gaggccttgt tctcgttcgt gcaggccgtc gtgagcaaca
      361 gcgcaacggg cgtcaggccc gtggtcgcga tgccgaaccc gttctatcaa atctacgaag
      421 gcgcggcgtt gctcgccggc gccgagcccc agttcctcaa caccaccgcc ggcaaccgct
      481 ttcagcccga tctcgactcc gtgcccgccg agatctggaa acgctgcgag atcctgtttc
      541 tctgcagccc tggcaatccc acgggcgcag tgttgtcgct cgaattcctg cgccatgcgc
      601 ttcgcctcgc ggacaaacac gacttcgtca tcgcctccga tgagtgttac gcggagctgt
      661 atcgcgacga aaacgccccg ccgccttcgt tgctccaggc agcactcgcg aatggccacg
      721 aatccttcca gcgctgcatg gtgttccatt cgctgtccaa gcgatcgagc gtcccgggac
      781 tgcgctcggg cttcgtcgcc ggcgatccgg cgttgatcaa gccctatctt ctctaccgca
      841 cctatcacgg ctgcgccatg ccggtgccga cccagctcgc cagcatcgcg gcctgggacg
      901 acgacgcgca tgcggccgcc aaccgcgtgc tgtaccgcga gaaatacgca cgcgtgctgc
      961 cgatcctggc gccggtgctc gacgtggtgg agcccgacgg cgcgttctac ctctggcccg
     1021 atgtgcagcg cgacgacgaa tcgttcactc gcgacttgta tgccacgcag aatctcacca
     1081 tcctgcccgg cagctacctc gcccgggaca cgcgcgcggg caaccccggc cggcagcgcg
     1141 tgcgcatctc gctggtcgcg ccggtcgaag aatgtgtcgt cgccgcgcag cgaatccgaa
     1201 cttttctgga gacaacctaa gtgaccgacc tgactactct cgccgccacc atcgacgagg
     1261 ccttcgagcg acgcgcggag ctgaccgcga agtccgcgcc cgccagcgtg cgtcatgccg
     1321 tcgaagagag catcgagctc ctggactcgg gccaggcgcg cgtggcggaa aagaaagacg
     1381 gtcagtggac ggtgaaccag tggctcaaga aagccgtgct gctgtatttc cgcacgcacg
     1441 acaacaccat catggacgcc ggctacacgc gtttcttcga caaggtgccg ctcaagtacg
     1501 ccgcgcgctc acaggctgac gacggcgatg ccctgcgcaa cggtggcacg cgcgtcgtcc
     1561 cgccagccgt cgcgcgccga ggctcgtaca tcgcgccaaa caccgtcctc atgcctagct
     1621 acgtgaatat cggcgcctac gtggacagcg gcaccatggt cgacacctgg gccacggtcg
     1681 gctcctgcgc gcagatcggc aagaacgtcc acctttccgg cggcgtgggc atcggcggcg
     1741 tgctcgagcc gctgcaggcg tcgcccacca tcatcgaaga caactgcttc atcggcgcac
     1801 gctcggaagt cgtcgaaggc gtgatcgtcg aagaaggctc ggtcatcagc atgggcgtgt
     1861 acatcggtca gagcacgcgc atctatgacc gcgccacggg ccagatcctg cagggacgta
     1921 tccccgccgg ctccgtggtg gtgtcgggca acctgccctc gtccgacggc aagtattcgt
     1981 tgtactgcgc ggtcatcgtc aaacgagtcg acgcgcagac acgtgcgaag acatccatca
     2041 acgaactgct gcgcgccgac tgacctggtg cacgcgcgcg ctgcacaatt gatcgcggaa
     2101 ctgggtctcg aaccccatcc cgaaggtggc tactaccgcg agatcttccg ctccgcgcgc
     2161 ctggtgacgc cgctcgacgg gcgcggtgcg cgcgcggcgc tcacgactat ctacttcctg
     2221 ctgccaggcg gagcttgcag ccgctggcat cgcgtgacct cggacgaggt gtggcacctc
     2281 tacgagggcg gccccctgga agtactcgag atcgatgccg gcttgagcca actcacgcgc
     2341 caccagctcg ttgtggcttc agaaactccg gtctgcacca tcgccgccga ctgctggcag
     2401 gccgcgcgtg cactcgccga ctacgcactg gtcggctgca ccgtgggccc gggtttcgat
     2461 ttcgcggatt tcacgctgct cgc
//
LOCUS       DATDEU010000058         4622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1031072, whole genome shotgun sequence.
ACCESSION   DATDEU010000058 DATDEU010000000
VERSION     DATDEU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031072"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VIV63_01295"
     CDS             <1..1253
                     /locus_tag="VIV63_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123256.1"
                     /translation="LTVTPGADRFVADVLLESRLVRVQGGVPAARPTRTPASASGASI
                     GYTPSNPDGDDGGPITDYDIIGSAQDGTGLFALHAAPDINLLCIPPLARETDVGSSTL
                     LVAGRFCRERRCMLIVDPPSSWASSAAALDGMRHWPFRSEDAAMFFPRVVAFDRLRGR
                     FELFAPCGVAAGIIARCDESTPVWAAAEGDAPILRHGMRPAWNVNNADRARLANAGIN
                     LLQAVRVAPKFATSPRTLASGSAAVSDWRYLSARRLALFILTSIERGTRWMLFERNAQ
                     PTWGFAKAQVEAFLDSLYAEGAFAARSGELSYFVVCDERINTPETMRAGKINLAFGFA
                     ATKPGEYHAFIVAHSPSSSRGKVISVNRFANYSPSVDLEIESSILRGLVPLAPVVPPL
                     GPVHEPVEPAAELAEAPLAPYLEA"
     gene            complement(1257..2264)
                     /locus_tag="VIV63_01300"
     CDS             complement(1257..2264)
                     /locus_tag="VIV63_01300"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY6123257.1"
                     /translation="MTSGISREQRESLARFAKASNPRALWQLANTLLPFLALWGCMAW
                     TLLAGVSYFWTLLLALPTAALYVRLFILQHDCSHGSFLGSARANRWLGASLGLLTLFP
                     FAYWKKTHGVHHATSGNLDQRELGDIRTLTVSEYRASSRWMRFCYRFYRSMPVMLGIG
                     PFYQFVAKHRFPLGMPWSWKKEWRSVALNNLSLLALGVGLSIWLGWGVVLAAHLPVVA
                     IAGALGVWLFYVQHSFEESYWVRRADWDPRAAAVDGSSFYDLPAVLRWITGNIGYHHI
                     HHLAPRIPNYHLRAAHDAAPALRPAVRLRLRDSLACARMKLWDESLGRMVGFPRFGRG
                     V"
     gene            2376..3119
                     /locus_tag="VIV63_01305"
     CDS             2376..3119
                     /locus_tag="VIV63_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY6123258.1"
                     /translation="MTSTTSGKASADTALAERVDALDWPELQSALDERGSAIVLRLLD
                     AQQCRALATMYSDDDAFRSRVIMAKHGFGRGEYRYFSYPLPPRVGILREVFYPRLAAI
                     ANRWNEQLGVETRFPATQAEFLRRCHDAGQMRPTPLLLQYGAGDYNCLHQDLYGEHVF
                     PLQIAVLLSEPGEDFSGGEFVLTEQRPRRQTRVEVCPLRQGDAIIFAVHQRPARGTRG
                     VYRVNMRHGVSELRSGKRHTLGVIFHDAA"
     gene            complement(3097..3315)
                     /locus_tag="VIV63_01310"
     CDS             complement(3097..3315)
                     /locus_tag="VIV63_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEY6123259.1"
                     /translation="MPGTLGGHRKTQIYGRLDCRTALAAIARGGYVNQRVFFLDAKAA
                     TAAGYRPCAVCLPEAYARWKKLTPHRER"
     gene            complement(3372..3917)
                     /locus_tag="VIV63_01315"
     CDS             complement(3372..3917)
                     /locus_tag="VIV63_01315"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HEY6123260.1"
                     /translation="MARVATDVQPARPQMDWLTMNTTTTAGPTLRAWARARSSAFGRW
                     LFARTVSKRAPYFGTIRPKFLDLEPKLCRVTMKKRRAVENHIGTVHALAMGNLCELAA
                     GMCTEVTIPVGMRWIPRGMTIEYLAKAESDVVATARLDKTEWAGPENVGVPVTVTDTA
                     GKEVVRAVISMYVSPKRETAG"
     gene            3964..4323
                     /locus_tag="VIV63_01320"
     CDS             3964..4323
                     /locus_tag="VIV63_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY6123261.1"
                     /translation="MTKDTQANSADARAASIRKAVRAIKRGTFASYGEIARRAGLPGR
                     ARLVGQVLRDSADLRLPWHRVTGAQGRIAFPAGSASAHKQLRLLAAEGVKIRGNRALA
                     APIAGGRRSLDALLWKI"
     gene            4331..>4622
                     /locus_tag="VIV63_01325"
     CDS             4331..>4622
                     /locus_tag="VIV63_01325"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6123262.1"
                     /translation="MTDRYVVFSHGKDAGPWGVKITALAGNARSEGYEVESVDYRGVD
                     DPRERVNKLVEYCKELKGDLVLCGSSLGGYVSVAAASLLHARGVFLMAPALTM"
BASE COUNT          752 a         1516 c         1561 g          793 t
ORIGIN      
        1 gcctcaccgt cacgccgggc gccgaccgct tcgtggccga cgtcctgctc gaatcgcgcc
       61 tggtgcgggt gcagggcgga gtgcccgccg cgcggcccac gcgcacgccg gcttccgcct
      121 ccggtgcctc catcggctac acgccatcca atcccgatgg cgatgacggc gggccgatca
      181 cggactacga catcatcggt tcagcgcagg acggcaccgg cctcttcgcg ctgcatgccg
      241 cgcccgacat caacctgctg tgcattccgc ccctggcccg ggaaaccgac gtcggttcgt
      301 ccacgctgct ggtggccggg cgtttctgcc gcgaacggcg ctgcatgctg atcgtcgatc
      361 cgccgtcgtc gtgggcctcg agcgccgcgg ctctcgatgg catgcgtcac tggccattcc
      421 gcagcgaaga tgcggcaatg ttcttcccgc gcgtggtggc tttcgaccgg ttgcgcggcc
      481 gcttcgagct gttcgcgccc tgcggtgtgg cggccggcat catcgcgcgc tgcgacgaga
      541 gcactcccgt ctgggcagcg gccgaaggcg acgctcccat cctgcgtcac ggcatgcgcc
      601 ccgcatggaa cgtgaacaat gccgaccgtg cgcggctcgc gaacgccggc atcaacctgc
      661 tgcaggcggt ccgcgtggcg ccgaaattcg ccaccagccc ccgcacgctc gccagcggct
      721 ccgccgcggt gagtgactgg cgctacctgt cggcgcgccg gctcgcgctg ttcatcctga
      781 cgtcgatcga gcgcggcacg cgctggatgc tgttcgaacg caatgcccag cccacctggg
      841 gattcgcgaa ggcccaggtc gaagccttcc tcgattcgct gtatgccgag ggtgcgttcg
      901 ccgcgcgcag cggcgagctc agctatttcg tggtgtgcga cgagcgcatc aacacgccgg
      961 aaaccatgcg cgccggcaag atcaatctgg ctttcggatt cgcggccacc aagcccggcg
     1021 agtatcacgc cttcatcgtc gcgcattcgc ccagcagttc gcgcggcaag gtgatcagcg
     1081 tgaatcgctt cgcgaattat tcgccgagcg tggacctcga aatcgagtcg agcatcctgc
     1141 gaggcctggt gccgctggcg cccgtcgtgc cgccgctcgg cccggtccat gagccggtgg
     1201 agccggccgc ggaactcgcc gaagcgccgc tcgcgcctta cctcgaagcg tagatcctag
     1261 actccgcgtc cgaagcgcgg aaatccgacc attcttccca ggctctcgtc ccagagcttc
     1321 atgcgcgcgc acgccaggct gtcgcgcagc cgcaggcgca cggcggggcg caacgcgggc
     1381 gcggcatcgt gcgccgcccg caggtggtag ttaggaatgc gtggcgccag gtgatggatg
     1441 tggtgatagc cgatgttgcc ggtgatccag cgcaacacgg cggggagatc gtaaaacgag
     1501 ctgccgtcga cggcggcggc gcgaggatcc cagtccgcgc ggcgcaccca gtaactctcc
     1561 tcgaagctgt gctgcacgta gaacagccag acgccgagcg ccccggcgat ggccaccacc
     1621 ggcagatgcg cggccagcac gacgccccag ccgagccaga tgctcaggcc cacgcccagc
     1681 gcgagtaacg agaggttgtt gagcgctacg ctgcgccatt ccttcttcca gctccacggc
     1741 atgccgagcg ggaagcggtg tttggcgacg aactggtaga acggcccgat gccgagcatc
     1801 accggcatgc tgcgatagaa gcgatagcag aaacgcatcc agcgggagct cgcgcggtat
     1861 tcgctgacgg tcagcgtgcg gatgtcgccg agttcgcgct gatcgaggtt gcccgaggtg
     1921 gcgtgatgca cgccgtgggt cttcttccag tacgcgaacg ggaacagcgt cagcagtccc
     1981 aggctcgcac ccagccagcg attggcgcgt gcgcttccca ggaacgagcc gtggctgcag
     2041 tcgtgctgga ggatgaagag ccgcacgtag agcgccgcgg ttggcagcgc cagcagcaac
     2101 gtccagaagt agctgacgcc cgcgagcagc gtccacgcca tgcatcccca caaagcgagg
     2161 aatggcagca gcgtgttggc gagttgccag agtgcgcggg ggttggaggc ttttgcgaag
     2221 cgcgcgagcg attcacgttg ctcgcgggaa ataccagagg tcatggccga ttctgcgccc
     2281 ttcggaccgg gaaggtcagc aagtttcgga atgctgtgtg cgcttccgct cattaaattg
     2341 cccggctcgc ccgcgcggca gccactggag gaagcgtgac gagtactact tcaggcaagg
     2401 catccgctga cacggcgctg gccgagcgcg tggacgcgct cgactggccc gaattgcagt
     2461 cggcgctcga tgagcgcggc agcgcgatcg tcttgcgatt gctggatgcg caacagtgtc
     2521 gcgcgctggc cacgatgtac tccgacgatg acgctttccg cagccgcgtc atcatggcga
     2581 aacacggatt cgggcgtggc gaataccgct acttctccta tccgttgccg ccgcgcgtcg
     2641 gaatccttcg agaagtgttc tatccgcggc tcgcggccat cgccaatcga tggaacgagc
     2701 aactcggtgt cgagacgcgg tttcccgcca cgcaggcgga atttcttcgg cgttgccacg
     2761 acgccgggca aatgaggccg acaccgctgc tgttgcagta cggcgcgggc gactacaact
     2821 gcctgcacca ggatctctac ggcgaacacg tgtttccact gcagatcgcc gtgctgcttt
     2881 cagagccggg cgaggatttc tccggcggcg agttcgtgct gaccgagcaa cggccgcgcc
     2941 ggcagactcg cgtcgaggtg tgtccgctgc gccagggcga cgcgatcatt tttgccgttc
     3001 accaacgccc cgctcgcggt acgcgaggcg tgtatcgcgt gaacatgcgt cacggcgtca
     3061 gcgagcttcg aagcggcaag cggcacacgc tgggcgtcat ctttcacgat gcggcgtgag
     3121 ttttttccat cgcgcatacg cttccggcaa acacaccgcg caaggccgat agccggccgc
     3181 ggtagccgcc ttcgcatcca ggaagaacac gcgttgattc acgtagccgc cgcgagcgat
     3241 ggccgcgagc gcggtccggc agtcgaggcg gccatagatc tgcgtcttgc ggtgtccgcc
     3301 gagagtgccg ggcaccgggc tcgtgtacgg cgcgccgtcg gcgccgatca gcgtccaggt
     3361 ccgcgcggtg gtcagcccgc ggtctcgcgc ttgggcgata cgtacatgct gatgaccgcg
     3421 cgcacgactt ccttgccggc ggtgtcggtg accgtgacgg gcacgccgac gttttctggc
     3481 ccggcccatt ccgtcttgtc gagacgcgcg gtggcgacga cgtcggactc ggctttcgcc
     3541 aggtattcga tggtcatgcc gcgcgggatc cagcgcatgc cgacgggaat ggtgacttcg
     3601 gtgcacatgc ccgcggcgag ctcgcagaga tttcccatcg cgagcgcatg cacggtgccg
     3661 atgtgattct cgacggcgcg acgcttcttc atcgtgacgc ggcacagctt gggttcgaga
     3721 tcgaggaact tcggccggat ggtgccgaag taaggcgcgc gctttgaaac cgtacgcgcg
     3781 aacagccagc gtccgaatgc ggaactccgc gctcgcgccc aggctcgaag cgtgggtccc
     3841 gcggtggtcg tggtgttcat ggtcaaccag tccatctgcg gacgggcggg ctgtacgtcg
     3901 gttgctactc gggccatcgc gtgatcgaat cttcagcgcc gcggggcttg gactgtagtg
     3961 gacatgacga aagacacgca agcgaactcg gcggatgcgc gcgccgcgtc aattcgcaaa
     4021 gccgttcgcg ccatcaaacg cggcaccttc gccagctacg gcgaaattgc gcgccgcgca
     4081 ggtttgccgg gccgcgcgcg cctggtgggg caggtgctgc gggactccgc ggacctgcgc
     4141 ttgccctggc atcgtgtcac cggcgcgcag gggcgcatcg cctttcccgc ggggtcggcg
     4201 agcgcacaca aacaattacg tttgctggcc gcggaaggtg tgaagatccg cggcaatcgc
     4261 gcgctggccg cgcccatcgc gggcggccgg cgcagtctcg atgcgctgct ctggaaaatc
     4321 tgaggacctc atgacagatc gttacgtcgt tttctcccat ggcaaggatg ccgggccctg
     4381 gggcgtgaag atcaccgcgc ttgccggcaa tgcgcgcagc gaaggatatg aagtggagtc
     4441 agtcgactac cgcggcgtcg acgatccgcg cgagcgggtc aataaactgg tggagtactg
     4501 caaggagctc aagggcgacc tggtgttgtg cggctcgagt ctgggtggat acgtttcggt
     4561 ggccgcggcc tcactgctgc atgcccgcgg cgtattcctg atggcgccgg cgctgaccat
     4621 gc
//
LOCUS       DATDEU010000059         3885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1905980, whole genome shotgun sequence.
ACCESSION   DATDEU010000059 DATDEU010000000
VERSION     DATDEU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905980"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /gene="dtd"
                     /locus_tag="VIV63_01330"
     CDS             complement(<1..304)
                     /gene="dtd"
                     /locus_tag="VIV63_01330"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY6123263.1"
                     /translation="MITLIQRVSEAAVSVDDRIVGRIGAGILALVGFERGDDKARVEK
                     LLTRVLTYRIFADDGGRMNLSLADTGGGLLLVPQFTLAADTHKGTRPGFSTAAEPDT"
     gene            391..1026
                     /locus_tag="VIV63_01335"
     CDS             391..1026
                     /locus_tag="VIV63_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6123264.1"
                     /translation="MALRISGLVLLLIAPFAVNAQVPAVTGDAKRGQELTYTCSGCHA
                     ISNYKNVYPTYSVPKLNGQRPEYLVAALKAYKSGERSHGTMHSHAASMTEQDMADAAV
                     YLAGPEVLTVSKTDVPAAQRPKASEVCLACHGTNGVGITPDYPTLSGQHRDYIERALV
                     DYQRGGRKNAVMAGMVATLTAKDIEELAAYYSSQKPALKVVPKKTSFLSSK"
     gene            complement(1036..2943)
                     /locus_tag="VIV63_01340"
     CDS             complement(1036..2943)
                     /locus_tag="VIV63_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY6123265.1"
                     /translation="MAFACHASQGAVEERGTLVLDNIPSVDTPLSAKLDDYMNARGAS
                     FVDWLPDGGILIATRFGDVEQLHRVAMPLGAREQLTFYREPVTSARAPQSAVAPGFVF
                     LKDQGGNEYAQIYWYDNATRAVRLLTDGKGLHGGVSWSHDGNRIAFHGTGRDSVSYDL
                     YIAEPANKFAQPRLVFNGFQKNWSVQDWSPDDSKLLINNVVSANESHLFVMDIATAAL
                     TPVGEGKDIARVQQAKFTADGRGVYLVTNRDSEFQQLRRIDLVTGVSEALTAHIPWDI
                     DSFARSDDGRYLAWVANVDGLSRLTVVNVANRTESLPPLPDGQIGRIAFDRSGKKLAL
                     SIESPQSPRDVFVLEVERNALVRFTKSEAGPIDPLQYVPAELMRFPTFDRENGKSRQI
                     PAFVFRPRTPGPHPVYIDIHGGPEAQYTPAFSPFTQFLVRELGFMVIAPNVRGSSGYG
                     NTYMNLDNGEDREDAVRDIGALIVWIGLQKDLDAKKVFVAGGSYGGYMSLASLVHFGD
                     RLRGGIDVVGISNFVTFLESTSAYRRDLRRPEYGDERLPRMRAYLQRISPLTNAARIS
                     KPLLVVQGLNDPRVPATESQQMVAKIRARGGEVWYLAAKDEGHGFRKKSNRDFYQKTV
                     VTFLEKQLAAK"
     gene            3007..>3885
                     /locus_tag="VIV63_01345"
     CDS             3007..>3885
                     /locus_tag="VIV63_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6123266.1"
                     /translation="MPRLTTFADGITAVDTEYVRPQMDASHIVVSADCAAVVDTGPNT
                     AVPLILAALEKLGLAADRVELLFLTHVHLDHAGGAGALMRALPRATCVVHPRGAPHMI
                     DPAKLIAGTRAVYGDELYSRLYGEILPVERERLVIAQDGQRFALAGRTFECVHTPGHA
                     LHHQAIVDHGANSIFTGDTFGLSYREFDTAKGAWIMPTTTPTQFDPGQLKASIVRLMQ
                     FRPRRVYLTHYSEVSDCARLANDMVDAIDEFVKIARAAGPDDLPRMRGDLRRMAHEGL
                     REHGCTMTDSQIDAILG"
BASE COUNT          667 a         1294 c         1244 g          680 t
ORIGIN      
        1 cggtatcggg ctcggccgcg gtgctgaagc cgggccgcgt tcccttatgg gtgtccgcgg
       61 ccagcgtgaa ctgcggaacc agcagcagcc cgccgccggt gtccgccagc gagaggttca
      121 tgcgtccgcc atcgtcggcg aagatccggt aggtgagcac acgcgtcagc agcttttcga
      181 cgcgcgcctt gtcgtcgccg cgctcgaaac cgacgagggc gaggatgcct gcgccgatgc
      241 gacccacgat ccggtcgtcg accgaaaccg ccgcctcgct gaccctttga atgagcgtta
      301 tcacccgagg attgtgcccg gtggcaattg cgcgcccaag tccttaatat cgcggcctgt
      361 tttcgggagc cacggggcaa tagttaagtc atggcattgc gtatttcagg tctcgttctt
      421 cttctgatcg cgccgttcgc ggtcaacgcc caggttcctg cggtcaccgg cgacgccaag
      481 cgcggccagg agctgaccta cacctgcagc ggctgccacg ccatttctaa ctacaagaac
      541 gtctatccga cgtattcggt gcccaagctg aacggtcagc gcccggaata cctcgtcgcc
      601 gcgctcaagg cttacaagag cggcgaacgc tcgcacggca ccatgcattc gcacgcggca
      661 tcgatgacgg agcaggacat ggccgatgcg gccgtctacc tggccgggcc cgaagtgctc
      721 accgtgtcca agaccgacgt ccccgccgcg cagcgtccga aggcctcgga agtctgcctc
      781 gcgtgccacg gcaccaacgg cgtcggcatc acgcccgact atccgacgct ctccggccag
      841 catcgcgact acatcgagcg tgcgctggtc gactaccagc gcggcggacg caagaacgcc
      901 gtcatggcgg gcatggtcgc cacgctgacc gcgaaggaca tcgaggaact cgcggcttac
      961 tactcgagcc agaagccggc gctgaaggtc gttccgaaga agacctcgtt cctgtccagc
     1021 aagtagtcag gccggctact tcgcggcgag ctgcttctcg aggaacgtga ccaccgtctt
     1081 ctggtagaag tcgcggttcg acttcttgcg gaaaccgtgg ccttcatcct tcgccgcgag
     1141 ataccagacc tcgccaccgc gcgcgcggat cttcgccacc atctgctggg attccgtcgc
     1201 cggcacgcgc gggtcgttca atccctgcac cacgagcagc ggcttcgaga tacgcgccgc
     1261 gttggtcagt ggcgaaatcc tctgtagata ggcacgcatg cgcggtagcc gctcgtcgcc
     1321 gtattccggc cggcgcaggt cgcggcggta ggccgaggtg ctctcgagga acgtgacgaa
     1381 gttggagatg ccgaccacgt cgatgccgcc acgcaggcgg tcgccgaaat gcaccagcga
     1441 cgccagcgac atgtagccgc cgtacgatcc gccggcgacg aacaccttct tcgcgtccag
     1501 gtccttctgc aggcctatcc acacgatcag cgcgccgatg tcgcgcaccg catcctcgcg
     1561 gtcttcgccg ttgtcgaggt tcatgtaggt attgccgtaa ccgctggagc cgcgcacgtt
     1621 gggtgcaatg accatgaagc cgagttcacg cacgaggaat tgcgtgaacg gactgaacgc
     1681 cggcgtgtac tgcgcttccg ggcctccgtg gatgtcgatg tacaccgggt gcggtccggg
     1741 tgtgcgcggg cggaatacga acgccgggat ctgacggctc ttgccgttct cgcgatcgaa
     1801 cgtgggaaac cgcatgagct ccgccggcac gtactgcaac gggtcgatgg ggcccgcctc
     1861 gctcttcgtg aaacgcacca gcgcgttgcg ctcgacctcg agcacgaaca cgtcgcgcgg
     1921 cgactgcggg ctttcgatcg acaacgcgag cttcttgccg ctgcggtcga aggcgatgcg
     1981 gccgatctgg ccgtcgggaa gcggcggcag gctttcggtg cgattcgcga cgttcaccac
     2041 ggtgaggcgg ctcaggccat cgacattcgc cacccaggcg agataacggc cgtcgtcgct
     2101 tcgcgcgaag gagtcgatat cccaggggat gtgcgcggtg agcgcctcgg acacgccggt
     2161 gacgaggtcg atgcgccgta actgctggaa ttcactgtca cggttggtga ccaggtagac
     2221 gccgcggccg tccgccgtga actttgcctg ctggactctc gcgatgtcct tgccttcgcc
     2281 caccggcgtc agcgcggccg tggcgatgtc catcacgaac aggtgggact cgttcgccga
     2341 gaccacgttg ttgatgagca acttgctgtc atcgggcgac cagtcctgca ccgaccagtt
     2401 cttctggaat ccgttgaaca cgaggcgcgg ctgggcgaac ttgttagccg gctcggcgat
     2461 gtagaggtcg tagctgacgc tgtcgcggcc cgtgccgtgg aacgcgatgc gattgccgtc
     2521 gtggctccat gagaccccgc cgtgcaggcc cttgccgtcg gtgagcaggc gcacggcgcg
     2581 cgtggcgttg tcataccagt aaatctgcgc gtactcgttg ccgccctggt ccttgagaaa
     2641 cacgaacccc ggggccacgg ccgattgcgg cgcacgcgcg ctggtcacgg gctcccgata
     2701 gaacgtgagc tgctcgcgcg cacccaatgg catggccacg cgatgcaact gctcgacgtc
     2761 gccgaaccgc gtggcgatga gaattccgcc atcgggtagc cagtcgacga agctcgcgcc
     2821 gcgcgcgttc atgtagtcgt cgagcttcgc cgacagcggc gtgtcgacgg acgggatgtt
     2881 gtcgagcacc agggtgccgc gctcttcgac ggcaccttgt gaggcgtgac aggcgaaagc
     2941 catgactagt ggcaggagta atttccgatt catgctgcta tgttgccgca agccatgaat
     3001 gcagtcatgc caagactgac gacattcgcc gacggaatca ccgccgtcga cacggagtac
     3061 gtgcggccgc agatggacgc ctcgcacatc gtcgtctccg ccgattgcgc ggcggtcgtg
     3121 gatacgggtc cgaacacggc cgttccgctg attctcgcgg cgctcgaaaa actcgggctg
     3181 gccgcggacc gcgtggagct gctgttcctc acgcacgtgc acctcgatca tgcaggcggg
     3241 gccggcgccc tgatgcgcgc gctgccgcgc gccacctgcg tggtgcatcc gcgcggtgcg
     3301 ccccacatga tcgaccccgc gaaactcatc gccggcactc gtgcggtgta tggcgacgag
     3361 ctgtattccc ggctgtacgg cgaaatcctg cccgtcgaac gcgaacgtct cgtgattgcc
     3421 caggatggcc agcgattcgc gctcgccggc cggaccttcg agtgcgtgca cacgccggga
     3481 catgcgttgc atcaccaggc catcgtcgat cacggcgcca acagcatctt cacgggcgac
     3541 accttcggct tgtcgtaccg cgagttcgac acggcgaagg gcgcgtggat catgcccacg
     3601 accaccccca cgcagttcga tcccgggcag ctcaaggcat ccatcgtccg gctcatgcaa
     3661 ttccggccgc ggcgcgtcta tctcacgcat tacagcgaag tcagcgattg cgcgcgcctc
     3721 gccaacgaca tggtcgacgc catcgacgaa ttcgtgaaga tcgcgcgcgc cgcggggccg
     3781 gacgatctgc cgcgcatgcg cggcgacctg cgccgcatgg cgcacgaagg cctgcgcgag
     3841 cacggctgca cgatgaccga ctcgcagatc gacgccatcc tcggc
//
LOCUS       DATDEU010000060         6841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_885230, whole genome shotgun sequence.
ACCESSION   DATDEU010000060 DATDEU010000000
VERSION     DATDEU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885230"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..972)
                     /locus_tag="VIV63_01350"
     CDS             complement(139..972)
                     /locus_tag="VIV63_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEY6123267.1"
                     /translation="MAAFRANGIITLTTDFGAREPFVGIMKGVMLGHAPHLRFIDMAH
                     EVSAFQPVEAGFWLAHALRYFPAGTLHVAVVDPGVGTPRALLVAFSGEQALLAPDNGL
                     LAPLAARGKIDRAIKVDPARLVKFGVSDVSATFHGRDVFAPLAAAIAAGRCDPSDLGT
                     DITSVSQDAWPPTSPRADGGVAGVIVAVDRFGNLISNIESSAVVALSTPTVHVAGLAL
                     PLKRTYGDAQPGEYMGLINSFEVLEVAQSRGNASKGLNVAVGAPISAVPAVLDPQFRR
                     G"
     gene            1004..4228
                     /locus_tag="VIV63_01355"
     CDS             1004..4228
                     /locus_tag="VIV63_01355"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY6123268.1"
                     /translation="MSRTTEPVNRIPTQVSVGTLALLCGCFVLSGIAALIYQTAWMRQ
                     FAIVFGTSELAVATVLAAYMGGLALGAALAERFLPRVSRPVLTYGMLEIGIAGSAVFI
                     VPLLLWLANIALQSFFGNQPEPPDSDHAGTTLFYLISAFVALALPTTLMGATLPVLAR
                     YAVAEEAQIGRRIGLLYAMNTAGAVGGALIAAFVLLPELGLQRTVWVGAALNGIVFLL
                     AVALARRVPYEKRAKGYSEQAATGPAPARVRKYSFRTFPGPAWVLPLMLLAGAVAFFQ
                     EVLWTRMLSHVVGSSIYAFGVMVASFLTGIALGGVIGAAVAKTRERATVTLAVALVAA
                     AAAAATAYLHLEDLLPSTAGLLINSDIHFGFLRVPNALFSGLLLLPMTIAIGMTYPLA
                     VRILASDAGEAAPASARVYAWNTVGAIAGSLAAGFILIPELKYEGAIQVAVWASAALG
                     VIAAWTLLPVNRIVAIATTVIAVAGCVVFKPQPPMTLLVTSPLNVGNKHRVLYYDIGR
                     SASVVMLAQDGGLGLRTNGLPEALMESPGSLQRFSGEYWLSPLAVIARPRARDMLIVG
                     YGGGVVIEGVPPTVRKIDVIEIEPKVIEANDRTSAMRKRNPRTDPRVNVILNDARGAL
                     RLTHRRYDAIVSQPSHPWTAGASHLYTREFMQLAHDHLNLDGVFVQWMNVIFMDEDLM
                     RSLTATLLSVFKEVRIYRPDPNTVVFLASDQPLDLELNLAQSGNPLRGAPLHYSRFGI
                     NNVEDLLAALVLDADGARRLASGAPLITDDDNRIATSSIYERGRGMTGETSGRLVAAM
                     DPLQRADSVAYGPLKDVVSYPYIARRISVFSILDASLSDRIARMAQILDGDARGEYLR
                     AFYYRMNRQSQRSTELLRLAIDAYPTDDAVRLDFLRGWFVDLASNKAPPEIAEVAAGV
                     NPPAASILTAARHASRNEWREVALADEQLAEIPWTHTWYAEAVELRVNWRIRITSEKE
                     RKRYGDEAILMIDRLATMSPTLNLYGLRSRAGFAAGRPDVVVESVSNYARLALGMVRA
                     GINTATATAQDARALTAILDQAAKLPGVDAARVAEVRDEIKAIS"
     gene            complement(4229..5263)
                     /locus_tag="VIV63_01360"
     CDS             complement(4229..5263)
                     /locus_tag="VIV63_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY6123269.1"
                     /translation="MRWKLFALLAGGAACANAAQYPLVDAEALMFGDVETITAHGEDT
                     LPDLARRYGLGYEEILRANPGVDTWLPGEGTVIVIPGQRLLPPGPREGVVVNLPEHRL
                     YYFPKPKKGEIPQVITYPVSIGKMDWNTPLGKTRIVDKRKNPTWTPPESVRKEHAERG
                     DPLPPVVKAGPDNPLGAYAMRLGITPGAYLIHGTNNPIAVGMAITHGCIRMYPEDIEG
                     LFPLVPLNTPVWLINEPVKVARVGGQVWLEVHPPVDAEGQRAEVDLEGFYARANTALG
                     ETPAAIHWDFVLSTLKDASGLPQMIGLEMDPDMVPPPSPPPDTTPAPDAPAEPVPALV
                     PEATPVPPVR"
     gene            5330..5866
                     /locus_tag="VIV63_01365"
     CDS             5330..5866
                     /locus_tag="VIV63_01365"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY6123270.1"
                     /translation="MNDEQLAYLRQSVRTIADYPKPGIQFRDITTLLRDARAFEITVD
                     AMIEPWRGRQIDKVAGIEARGFILGGAVARGLQCGFVPIRKRGKLPHATVRVAYSLEY
                     GVDEMEMHKDAVRDGENVLLLDDLIATGGTATAAAQLLQSAGAQVVASSFVIDLPGLG
                     GADRLRNSGIAVHSLLTF"
     gene            5873..6592
                     /locus_tag="VIV63_01370"
     CDS             5873..6592
                     /locus_tag="VIV63_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6123271.1"
                     /translation="MRRHSFVLIAGAWHGAWCWSRVLPLLESQGHRVITPELPGTGTD
                     RTDAARVTLESWACFLADVLTREPEPVTLIGHSRGGIVISRAAELVPERIRRLVYLSA
                     YLLPSGRSLAEAARADVQSLVPPNMVPAESGHTCNLRPEVIREALFGHCTDADVDYAL
                     AHLSPEPLKPLVTPLKVTPERFGSVPRVYIECLQDRTVTLAAQRRMQSELPCDPVYAL
                     DSDHSPFLSHPQDLALLLGGL"
     gene            complement(6578..>6841)
                     /locus_tag="VIV63_01375"
     CDS             complement(6578..>6841)
                     /locus_tag="VIV63_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6123272.1"
                     /translation="CIHVAQRASYDATSRSEVNPADPNDHGSGSVDHIAFAATDYDEL
                     IASFTRHGLKFRATQVPGKDLRQLFVFDPDGIQIEINIRSQPA"
BASE COUNT         1145 a         2295 c         2187 g         1214 t
ORIGIN      
        1 aactcttcgt ctaaatcgaa gctttcgtta tcccgttcgg acatcgtttt ttcttcagaa
       61 aaactatttg aaacagtgag ttacgagccc ggcgggctcg caatgaggct gcgaactata
      121 gccgattcgc gttcgatatc aaccccgtcg gaattggggg tccagaaccg cgggaacggc
      181 cgaaataggc gcccccacgg ccacattcaa gcccttggac gcgttaccgc gcgactgggc
      241 gacctccagg acctcgaacg agttgatcag gcccatgtat tcgccgggct gggcatcgcc
      301 gtaggtgcgt ttcaggggca gcgcgagccc cgcgacgtgg actgtgggag tcgacaaggc
      361 gaccacggca gacgattcga tgtttgaaat gaggttgccg aagcgatcga cggcgacgat
      421 gactcccgcc actcccccgt cggcgcgcgg gctggtcggt ggccacgcgt cctgactcac
      481 ggatgtgatg tcggtcccga gatccgaagg atcgcaccgc ccggccgcga tggcggccgc
      541 gagcggcgcg aatacgtccc ggccgtggaa ggttgcgctc acgtcgctca cgccgaattt
      601 aaccaggcgc gccggatcga ccttgatcgc ccgatcgatc ttgccgcgtg ccgcgagcgg
      661 ggctaacagc ccgttgtccg gcgcgagcag cgcctgctcg cccgagaacg ccaccagcaa
      721 cgcgcgcggt gttccgacgc cgggatcgac cacggccacg tgcagtgtgc cggcgggaaa
      781 gtaacgtaat gcgtgcgcca gccagaagcc ggcttcgaca ggctggaacg cgctgacctc
      841 gtgggccatg tcgatgaaac gcaggtgcgg cgcgtgaccc agcatcacgc ccttcatgat
      901 tccgacgaag ggttcgcgcg cgccgaaatc ggtggttagc gtgatgattc cgttagcgcg
      961 aaaggctgcc atgaaaactc cgcgatccgt acactccgcg accatgtcgc gaaccaccga
     1021 acctgtcaat cgcatcccca cgcaagtttc cgtcggcact ctcgccttgt tgtgcgggtg
     1081 tttcgtactg agcggcatcg cggcgctcat ctatcagacg gcatggatgc gccagttcgc
     1141 gatcgtgttc ggcacttccg aactggccgt ggccacggtg ctcgccgctt acatgggagg
     1201 gcttgcgctc ggcgcggcgc tcgctgaaag attcctgcca cgcgtctcga ggccggtact
     1261 cacctacggc atgctggaaa tcggcatcgc cggcagcgcc gttttcatcg tgccgctgct
     1321 gctgtggctg gcgaacatcg cgctccaatc gttcttcggc aaccagcccg agccgcccga
     1381 cagcgaccac gccggcacca cactcttcta tctgatcagc gccttcgtcg cgctggcgtt
     1441 gccgaccaca ctcatgggag cgacgcttcc cgtgctggcg cgatacgcgg tcgccgagga
     1501 agcgcagatc gggcgacgca tcgggctgct gtatgcgatg aataccgcgg gcgcggtcgg
     1561 gggcgcgctc atcgccgcat tcgtgttgct gcctgaactg ggtctccagc gcaccgtgtg
     1621 ggtgggagct gcgctcaacg gcatcgtctt cctgctggcg gtcgcattgg cgcgtcgcgt
     1681 gccgtacgag aagcgcgcga aaggttactc cgagcaagcg gcgacgggcc ctgccccggc
     1741 gcgcgtccgc aagtactcgt tcaggacatt tccgggaccg gcctgggtat tgccgctgat
     1801 gctgctggcg ggcgccgtgg catttttcca ggaggtgttg tggacgcgaa tgctgtcgca
     1861 cgtcgtcggc agcagcatct acgcgttcgg cgtcatggtc gcgagcttcc tcaccggaat
     1921 cgcgctgggc ggtgtcattg gcgcggccgt ggccaagacc cgcgaacgcg cgaccgtcac
     1981 gctggccgtg gcgctcgtgg ccgccgcggc ggccgccgca acggcctatc tacacctcga
     2041 ggatctcctg ccatccacgg cgggcctgct gatcaactcg gacatccact tcgggttcct
     2101 gcgtgtgccg aacgcgctgt tctcgggcct gttgctgctg cccatgacca tcgccatcgg
     2161 catgacctat ccactcgcgg ttcgcatcct cgccagcgac gccggggaag ccgcgcccgc
     2221 cagcgcgcgc gtgtacgcgt ggaacaccgt cggagccatt gccggctcgc tggccgcggg
     2281 attcatcctg attccggaat tgaagtacga aggcgccatc caggtcgcgg tctgggccag
     2341 cgcggcgctc ggcgtcatcg ccgcgtggac gcttttgccg gtgaatcgca tcgtggccat
     2401 cgccaccacc gtgatcgccg tcgccggctg cgtggtcttc aaaccgcagc cgccgatgac
     2461 cctgctcgtc acttcaccgc tcaacgtcgg caacaaacat cgcgtgctct actacgacat
     2521 cggccgcagc gcgagcgtcg tgatgctcgc gcaggatggc ggattgggat tgcgcaccaa
     2581 cggcctgccc gaagcgctca tggaaagccc gggatcgctg cagcgattca gtggtgagta
     2641 ctggctgtcg ccactggcgg tgatcgcgag accgcgcgcg cgcgacatgc tgatcgtcgg
     2701 ttacggcggc ggcgtggtca tcgaaggcgt accgcccacg gtgcgcaaga tcgacgtgat
     2761 cgagatcgag cccaaggtca tcgaagcgaa cgatcgcacc agcgcgatgc gcaagcgtaa
     2821 cccgcgcacc gacccgcgcg tcaacgtcat cctcaacgac gcacgcggcg cgttgcgcct
     2881 tactcatcgg cgctacgacg ccatcgtctc gcagccctcg catccgtgga cggccggcgc
     2941 ttcgcatctg tacacgcgcg aattcatgca gctcgcgcac gaccatctga atctcgatgg
     3001 tgtcttcgtg cagtggatga acgtgatctt catggacgaa gatctgatgc gctcgttgac
     3061 cgcgaccctg ctgagtgtct tcaaagaggt acgcatctac cggcccgatc cgaacacggt
     3121 ggtgttcctc gccagcgatc agccgctcga tctggaattg aacctcgcgc agagtggcaa
     3181 tcctctgcgc ggcgccccgc ttcactactc gcgcttcggc atcaacaacg tcgaagacct
     3241 gctcgccgcg ctggtgctcg acgccgacgg cgcgcggcgc ctcgcctccg gcgcgccatt
     3301 gatcaccgac gacgacaacc gcatcgccac gtcgagcatc tacgagcgcg ggcgtggcat
     3361 gacgggcgaa accagcggcc ggctggtcgc cgcgatggac ccgctgcaac gcgcggattc
     3421 ggtcgcctac gggccgctca aggatgtggt ctcgtatccc tacatcgcgc gccgcatcag
     3481 cgtgttctcc atcctggatg ccagcctgtc ggaccgcatc gcgcgcatgg cgcagatcct
     3541 cgatggcgat gcgcgcggag aatatctgcg cgccttctac taccgcatga accggcagtc
     3601 gcagcgctcc accgaactgc ttcggcttgc gatcgacgcg tatcccaccg acgacgccgt
     3661 gcggctcgac ttcctgcgcg gctggttcgt cgatctcgcg agcaacaagg cgccgccgga
     3721 aatcgccgag gtggccgcgg gcgtgaatcc tcccgctgcc agcatcctga ctgccgcgag
     3781 acacgcctcg cgcaacgaat ggcgcgaagt ggcgctggcc gacgagcagc tcgcggaaat
     3841 tccctggaca cacacctggt atgcggaagc ggtcgagttg cgcgtcaact ggcggattcg
     3901 catcaccagc gagaaggaac gcaagcgcta cggcgacgaa gccattctca tgatcgaccg
     3961 gctcgccacc atgagtccga cgttgaatct ctacggcttg cgctcgcgcg cgggtttcgc
     4021 ggccgggcgt cccgacgtgg tcgtcgagtc cgtctcgaac tacgcgcgcc tggcgctggg
     4081 aatggtgcgc gccggcatca acaccgccac ggcgaccgcc caggatgcca gggctttgac
     4141 cgcgatcctc gaccaggccg ccaaacttcc gggcgtggac gccgcgcgcg tcgccgaagt
     4201 gcgcgacgag atcaaagcga tttcctgatc agcgaaccgg tggtaccggc gtcgcctcgg
     4261 gcaccagcgc gggcacgggt tcggcgggag catccggcgc cggcgtggta tcgggcggcg
     4321 gtgacggcgg cggcaccatg tccggatcca tttcgaggcc gatcatctgc ggcagaccgg
     4381 acgcatcctt gagcgtcgac agcacgaaat cccagtggat ggcggccggg gtctcaccca
     4441 gtgcggtatt ggcgcgtgcg tagaaacctt cgaggtccac ttccgcgcgt tgtccttcgg
     4501 catccacggg cggatgcact tcgagccaca cctggccgcc gacgcgcgcc actttcaccg
     4561 gttcgttgat cagccacacc ggcgtgttca ggggaacgag cgggaacaac ccctcgatgt
     4621 cctcgggata catgcggatg cagccgtgcg tgatcgccat gccgaccgcg atagggttgt
     4681 tagtgccgtg gatgagatag gcgcccggcg tgatgccgag acgcatggca taggcgccga
     4741 gcggattgtc cggccccgcc ttgaccaccg gcggcagcgg atcgccgcgc tccgcgtgtt
     4801 ccttgcgcac cgactcgggt ggcgtccacg tggggttctt gcgtttgtcg acgatgcgcg
     4861 tcttgccgag cggcgtattc caatccatct tgccgatgct caccgggtag gtgatgacct
     4921 gcgggatctc gcccttcttc ggcttgggaa aatagtagag gcgatgttcg ggtagattga
     4981 cgaccacgcc ctcgcgcgga cccggcggca ggagccgctg accgggaatc acgatgaccg
     5041 tgccctcgcc cggcagccag gtgtccacac ccggattggc gcgcaggatt tcctcgtaac
     5101 ccaatccata tcgccgcgcg agatccggca acgtgtcctc gccgtgcgcg gtgatcgtct
     5161 cgacgtcgcc gaacatgagc gcttcggcat ccaccagcgg atattgcgcc gcgttagcgc
     5221 aagccgcgcc gccggccagc agcgcgaaga gtttccaacg catcaaaaac accttgtact
     5281 gaaccatttt atggacacag tcttgcacaa atctctacac tcgaccgccg tgaatgacga
     5341 acaactcgcc tacctgcgcc agtcggtccg aacgattgcg gattacccaa aaccgggtat
     5401 ccagttccgt gacatcacga cgctgctgcg cgacgccagg gccttcgaaa tcaccgtcga
     5461 tgcgatgatc gaaccctggc gtgggcgcca gatcgacaag gtcgccggca tcgaagcgcg
     5521 cggattcatt ctcggcggcg cggtggcgcg tggtctgcag tgcggcttcg tgccgattcg
     5581 caagcgcgga aaacttcccc acgccacggt gcgtgtcgcc tactcgctcg aatacggcgt
     5641 cgacgaaatg gagatgcaca aggacgccgt ccgggacggc gaaaacgtgt tgctgctcga
     5701 cgatctcatc gccaccggcg gcacggcgac cgccgcggcg cagctgctgc agtccgcggg
     5761 cgcgcaggtc gtcgcgtcgt ccttcgtcat cgacctgccc ggcctgggcg gcgccgaccg
     5821 cctgcgcaac tcgggcatcg ccgtacattc cctgctgaca ttctgaaacg tcatgcgtcg
     5881 gcatagcttc gtgctgatcg cgggcgcgtg gcacggcgcc tggtgctggt cgagagttct
     5941 gcccctgctc gagagccagg gccaccgcgt gatcacgccc gaacttcccg gcacgggcac
     6001 ggaccgcacc gatgcggcga gagtcacgct cgagagctgg gcgtgcttcc tggccgatgt
     6061 gctgacccgc gagcccgaac ccgtgacgct catcggtcac agccgcggcg gcatcgtcat
     6121 cagccgtgcg gcggaactcg tgcccgaacg gattcgccgg ctcgtgtatc tctcggccta
     6181 tctactgccg tcggggcgca gcctggctga agccgcgcgg gccgatgtcc agtcgctggt
     6241 gccgcccaac atggttcccg cggaaagtgg ccacacctgc aatctgcgtc cggaagtgat
     6301 ccgtgaggcg ttgttcggcc actgcaccga cgccgacgtc gactacgcgc tcgcgcacct
     6361 gtcgccggag ccgctcaagc cgctggtgac accgttgaag gtgacgccgg agcgctttgg
     6421 cagcgtgccg cgcgtgtaca tcgaatgcct gcaggaccgc acggtgacac tggccgcgca
     6481 gcggcgcatg cagtcggaac tcccctgcga ccccgtctac gcactcgact cggaccattc
     6541 gcccttcctg tcgcacccgc aggatctggc gctcttgtta ggcgggctgt gagcggatgt
     6601 tgatttcgat ctggattcca tccgggtcga acacgaagag ctggcgcaga tccttgcccg
     6661 gcacctgcgt ggcgcgaaac ttcaggccgt gccgcgtgaa actcgcgatg agctcgtcgt
     6721 aatccgtggc cgcgaacgcg atgtgatcga cgctgccgct gccatgatcg ttcggatccg
     6781 ccgggttgac ctcgctgcgc gacgtcgcgt cgtagctcgc ccgctgcgcg acgtggatgc
     6841 a
//
LOCUS       DATDEU010000061         8738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_885654, whole genome shotgun sequence.
ACCESSION   DATDEU010000061 DATDEU010000000
VERSION     DATDEU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885654"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VIV63_01380"
     CDS             complement(<1..527)
                     /locus_tag="VIV63_01380"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6123273.1"
                     /translation="MIPQPFDLRAPDGRELAAHWFAATQRHGVIVMNGATGFPQAFYF
                     KLAQYAAERGYDVLLYDYRGMGFSTSGSPASEMVRMSDWGMLDMRAALQAGADRAQGQ
                     PVVTLGHSVGGQFLGLLRNHALARLHVQIATSVGFWRWESAPFKYLAWWFWRVHGPLM
                     LALKGYIPTGGGWAG"
     gene            complement(524..1420)
                     /locus_tag="VIV63_01385"
     CDS             complement(524..1420)
                     /locus_tag="VIV63_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6123274.1"
                     /translation="MADLKLKDLRYLVAVADTRHFGRAAERSFVSQPTLSAQLKKLEE
                     YLGVQLVERAPKRVSLTVAGEEIVERARRILESSEEIVELAKGHRDPLAGRLKLALLP
                     TIGPYLLPNVAGKLRKHLPRLELMLYEYQTDVMLEKLHSGEIDVGILALPVPMDGLDS
                     YELYKEPFTVAMPADHRLAGRASIKVSDLHHETLLLLEDGHCLRDQALEICSSTDMHE
                     KQDFRATSLETLRQMVASGVGITLLPELAGRGAYGAARGVAIKPFVKPVPTRTIGAVW
                     RKSSARRAVILALAKHIEAHAL"
     gene            1539..2078
                     /locus_tag="VIV63_01390"
     CDS             1539..2078
                     /locus_tag="VIV63_01390"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY6123275.1"
                     /translation="MLGIGQKFPQYSLTGVVSNDQAKAFQPFDQDTHKGKWRVVFFWP
                     KDFTFVCPTEIAAFGKLDKEFKARDAQLLGVSTDSEYVHHAWRSTKDELKTLPFPMLS
                     DIKRELSTELGILDANAGVAQRATYIVDPEGVIRFVYVTDLSVGRSPDEVLRVLDALQ
                     TDELCPCNWKQGEDTLKAA"
     gene            2125..2694
                     /locus_tag="VIV63_01395"
     CDS             2125..2694
                     /locus_tag="VIV63_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY6123276.1"
                     /translation="MNIEQIRDALPDYARDLKLNLGSVLTTNGAPGLSEKQIWSIALA
                     SSIAARNTGFARSVEAQARAHLADVEVEGAKAAAAIMGMNNIYYRFLHLVEDGEYQTM
                     PARLRMNVIANHGIDKIDFELISLAVSAVNGCGLCVTSHEKKLRSHDVSRESIQSAVR
                     IAATIHAVAVALEFEQSAPVQPATAELAA"
     gene            complement(2771..3391)
                     /locus_tag="VIV63_01400"
     CDS             complement(2771..3391)
                     /locus_tag="VIV63_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123277.1"
                     /translation="MINASSAERYVAGTLSEEELAEFEAAMIERPQLAADVNVRRRIK
                     AGLSLLEERKELDPLLEPEAGRPRYLRYAAAAAVMVVAIGLWTTWHTDLTPSAHALFA
                     SAETGGSKIAASFMLVRTRSADTPVFTVRPDKGLVRLQILVDDPAAVSFTARIGTEEI
                     SILHVVDGFAELYVDPRVLGSGSHTLSLKPASGAEQTFPFTLNVAP"
     gene            complement(3388..4047)
                     /locus_tag="VIV63_01405"
     CDS             complement(3388..4047)
                     /locus_tag="VIV63_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6123278.1"
                     /translation="MPFGHDALVQTPELSFNDLSHRIRAGDTKAEDALSRRIAPGITQ
                     IIIGMTGNFAQAQELCQETLIVILSRLRNQPLDDPDKLPAFVAQVARNLVIAERRKER
                     RRKTDVDSDAIDSVADVSEGQEVDAQRESAAVAIRAVLKEMKSPRDRTLLVRYYLRDE
                     DKKEICRELGLTEPSFNVVLFRARSRFLELLEKRGLSGGDLFCMAIFVVIATQQLWSR
                     A"
     gene            complement(4148..5125)
                     /locus_tag="VIV63_01410"
     CDS             complement(4148..5125)
                     /locus_tag="VIV63_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY6123279.1"
                     /translation="MRSVFLDFGTVSHDDLDTTQLERVLPGITLHATSTDAEVDERIA
                     GVEFILTNKLNITRARMRAVPGLRFIGLTATGTNNIDLEAARELGIAVCNIREYCTVS
                     VVQHVLGAILLLTHRFREYGQAAIDGTWSRSEQFTVPGAAIRELSGKVLGIVGYGTLG
                     KAVANAARDALGMRVLVAQRQGPASEDSTTQSSPVRVTLDELLRTADVLSLHCPLTPA
                     TTGLIGKRELALMKPDALLINTARGALVDLEALVEALKGGDLGGAAIDVLPKEPPTEG
                     SVLFEAGIPNLLVTPHIAWAAFEARQRAVDELALNVEDFLRGGRRGRVV"
     gene            complement(5138..8248)
                     /locus_tag="VIV63_01415"
     CDS             complement(5138..8248)
                     /locus_tag="VIV63_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6123280.1"
                     /translation="MSFLEFPIRRYQFTMVAFLCLVALGWYAFASVPREEDPYFKIPG
                     FTVAAIYPGADPKDLERLIAKPLEDRFAALDDVLKMETSIRDGVSFTAIEFLASTDAD
                     KKYDEVTRELNALRPEFPPDVQIIVRKFSPGLVNTVQIALLSDGAPYRELEDYARELK
                     DKLKTVEGVRTSESWAYPERELRVAVDLRRMSELNLSPAQLIGAVQSENANIPAGFVD
                     LGSRSFSLKTSGSYESLDQVRDTVIASIEGRIVRVSDVADVTWSTAAYSYTGRYKGKR
                     AVFVTANQKDGYNILDVRERVMAAANQFAADLPKRIKLEVGFDQSDNVRSRLNRLYFD
                     FGIAIALVLLTLLPLGFRAAGIVMVSIPLSLSFGLACLYFLDYSLNQLSIAGFVVALG
                     LLVDDSIVVVENIARHMRMGKSRVQAALDGTRQIFVAILGCTATLIFAFLPLLALPGT
                     AGKFIRVLPMAVVATIIGSLLLALFIIPFVASRVLKEDKEGEENPFLKRVMGVIHHYY
                     RPALHWCLARPKFTVVAAIGGSLLLSAALVPVIGSSLFPKADTPQFLIQVESPNGSSL
                     SETDRALHVVEAELAKMPEVKGWFANLGHGNPQIYYNHIQRNDAANYAEVFVQLHEYD
                     TDETPVQLQLLREKLERYPGARIYVKEFQNGPPISAPISVRVIGSDLAVIEALSHRVE
                     DLVKAIPGTRDVKNPLNVSRTNLKLRIDSRKAQQLGVQTAELDRAVRLAVAGVSVGTF
                     KDTTGEQYPIVVRTPITQQAEFAALEQVRVPSLGGTTLPLSQLASLEFEKAPTLIQRF
                     NRERAMTIDADARPGYNIGELTAQITDKLNEMDWPRGYTYDLGGEDEASQEAFGGIGI
                     AIIVAIFGIFAILVLEFGNFKSTLIVLTVVPLGVFGGLLMLLLSGNDISFVASIGFVA
                     LVGIEIKNSILLVDFTNQLRAQGVALDEAIEQAGEIRFLPILLTSATAIGGLLPLALQ
                     NIGLYSPMSWVIIGGLITSTLLARLVTPVMYKLIPPAIAVTSHSGGTLAAPAGLAPSP
                     ATRMED"
     gene            complement(8265..>8738)
                     /locus_tag="VIV63_01420"
     CDS             complement(8265..>8738)
                     /locus_tag="VIV63_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY6123281.1"
                     /translation="NVKIGDKGEIRMDAFPGQPMTGTIVEVSSAADERTGMFPIEVQF
                     DSPPPRLVSGLVTRLRLAPTTDAPPLTYVPMAALVEGDGDRASVFVVDGGKAQRRDVR
                     VAFITADSIALESGLATGEVVVTDGALFLENGEAIEVLRDTTQQAVNAPPTTTEG"
BASE COUNT         1595 a         2728 c         2856 g         1559 t
ORIGIN      
        1 aacccggccc agccaccacc ggtcgggatg taacccttga gcgcgagcat caacggacca
       61 tgcactcgcc agaaccacca cgccagatat ttgaacggcg cggactccca gcgccagaaa
      121 cccaccgatg tagcgatctg cacgtggagg cgcgccagcg cgtgattgcg cagcagcccg
      181 aggaactggc cgccgacgct gtgacccagt gtcaccacgg gctggccttg cgcgcgatcc
      241 gcgccagcct gcagcgccgc gcgcatgtcg agcatgcccc agtcgctcat acgcaccatt
      301 tccgaggcgg gactgccgga tgtcgagaac cccatgccgc gatagtcgta cagcaacacg
      361 tcgtacccgc gttcggctgc gtattgcgcc agtttgaagt agaacgcctg cgggaagccg
      421 gtcgcgccat tcattacgat cacgccgtgt cgctgcgtcg ccgcgaacca atgcgccgcg
      481 agctcgcggc catcgggcgc gcgcagatcg aacggctgtg gaatcacagc gcgtgagcct
      541 cgatgtgttt ggcgagcgcg aggatgaccg cgcgacgcgc gctcgatttg cgccacacgg
      601 cgccgatggt gcgtgtgggc acgggcttca cgaaaggctt gatggcgacg ccgcgcgccg
      661 ctccgtaagc tccgcgcccc gcgagttcgg gcaacagcgt gatgccgacg cccgaggcca
      721 ccatctggcg caaagtctcc aggctcgtgg cgcggaagtc ctgcttctcg tgcatgtcgg
      781 tgctcgagca gatctcgagc gcctggtcgc gcaggcaatg gccgtcctcc agcaacagca
      841 gagtttcatg gtggaggtcc gacaccttga tgctggcgcg acccgcgagc cggtgatcgg
      901 ccggcatggc caccgtgaac ggctctttat acagctcgta ggagtcgagg ccgtccatgg
      961 gcaccgggag tgccaggatt ccgacatcga tttctcccga atgcagtttc tcgagcatca
     1021 cgtcggtctg atactcgtac aacatgagtt cgagccgcgg caggtgcttg cgcagcttgc
     1081 cggccacgtt cggcagtaga tagggcccga tggtgggcag caaggccagc ttgagcctgc
     1141 cggccagcgg gtcgcggtgg cccttggcga gctcgacgat ttcctcgctc gattcgagga
     1201 tgcggcgcgc gcgttcgacg atttcctcgc ccgccacggt gagcgacacg cgtttcggcg
     1261 cgcgctcgac gagctgcacg ccgaggtatt cctcgagctt tttcagctgg gccgacagcg
     1321 tgggctgact cacgaagctg cgttcggccg cacgcccgaa gtgccgcgtg tcggcgacgg
     1381 cgaccaggta gcgcaagtcc ttgagtttga ggtcggccat agatgctctc tatcgagttt
     1441 atacaatcaa tcgattggct gtaatggggt gttccccgga gaatgcgccc caatccagcc
     1501 aggcaagccc ccggcaatag attcaacaag gagtagacat gttaggtatc ggccagaagt
     1561 ttccccagta ttcgctcacc ggcgtcgttt ccaatgacca ggccaaggct ttccagccct
     1621 tcgaccagga cacccacaag ggcaaatggc gcgtcgtctt cttctggccc aaggatttca
     1681 ccttcgtgtg cccgaccgag attgccgcgt tcggcaagct cgacaaggag ttcaaggcgc
     1741 gtgacgcgca gcttctcggt gtttcgaccg acagcgagta cgtgcaccac gcctggcgca
     1801 gcaccaagga cgagctgaag acgctgccgt tcccgatgct gtcggacatc aagcgcgagc
     1861 tctcgaccga actcggcatc ctcgatgcga acgcaggcgt tgcgcagcgc gccacgtaca
     1921 tcgtcgaccc ggaaggcgtg atccgcttcg tgtatgtcac cgacctgtcg gtcggccgca
     1981 gcccggatga agtgctgcgt gtactcgacg cactgcagac cgacgaactc tgcccgtgca
     2041 actggaagca gggcgaagac acgctgaagg cggcctgaga aggtccccga ttctgagaat
     2101 ggaaaaaggc aaaaggagcc agcgatgaac attgaacaga tcagggatgc gttgccggat
     2161 tacgcgcgcg atctcaagtt gaacctcggc agcgtgctga ccaccaatgg cgctccggga
     2221 ctcagcgaga agcagatctg gtcgatcgcg ctggcttcgt cgatcgctgc gcgcaatacc
     2281 gggtttgcgc gcagcgtcga agcgcaggcg cgcgctcatc tggcggacgt ggaggtcgag
     2341 ggtgcgaaag cggccgcggc catcatgggc atgaacaaca tctactaccg ctttctgcat
     2401 ctcgtggaag acggtgagta ccagaccatg cccgcgcgtc tgcgtatgaa cgtgatcgcg
     2461 aatcacggca tcgacaagat cgatttcgag ctgatctcgc tcgccgtgtc ggcggtgaat
     2521 ggctgcggtc tgtgcgtcac ttcgcacgag aagaagctgc gttcacacga tgtgtcgcgc
     2581 gagtcgattc agagcgcggt gcgtattgcg gcaacgattc atgccgtggc cgtggcgctg
     2641 gagttcgagc agagcgcgcc ggtgcaaccg gctacggcgg agctcgcagc ctgacagtga
     2701 taattggcgt cttccccctc ccttgacggc caacatgcgg cgtccggttt caccgggcgc
     2761 cgtttttctt tcagggggcg acgttcagcg tgaacgggaa ggtttgttcg gcgcccgacg
     2821 ccggcttcaa cgacaaggtg tggctgcctg agccgagcac acgcgggtcg acgtagagct
     2881 cggcgaagcc gtcgacgaca tgcagaatcg agatttcttc ggttccgatg cgcgcggtga
     2941 atgacaccgc ggcggggtcg tccacgagga tctgaaggcg cacgagaccc ttatccgggc
     3001 gcacagtgaa cactggcgta tccgcagagc gggtccgcac cagcatgaaa ctcgctgcta
     3061 ttttcgagcc gccggtttcc gccgaggcga acagcgcgtg ggcggaggga gtcaggtccg
     3121 tgtgccaggt cgtccataga ccgatcgcga ccaccatgac ggctgcggca gccgcgtatc
     3181 tcaggtaacg cggtctgcct gcctcgggtt caagcaacgg gtcgagttct ttccgctctt
     3241 cgagaagtga caacccggct ttgatgcgcc gccgcacgtt tacgtcggcc gcgagctgcg
     3301 gccgctcgat catcgcggct tcgaattcgg ccagttcttc ctcagacagg gttcccgcaa
     3361 cgtagcgttc ggcgctggag gcgttgatca cgcccgactc caaagttgct gcgttgcgat
     3421 caccacgaaa attgccatac agaacaagtc tcctccgctg aggccccgct tttcgagcag
     3481 ctcgaggaag cggctgcggg cgcgaaacaa tacgacgttg aagcttggtt cggtcagtcc
     3541 gagctcccgg catatctcct tcttgtcttc atcccgcaag taataacgca ccaatagggt
     3601 tctatctcgc ggtgacttca tttccttaag gactgcccgg atagcgacag cggccgactc
     3661 gcgctgggcg tccacttcct ggccctcgga aacgtccgcc acgctgtcga tggcgtcgct
     3721 gtcgacatcg gtcttgcggc ggcgctcctt gcgtcgctcg gcgatcacca ggttgcgggc
     3781 cacctgggcg acgaaggccg gtagtttgtc cgggtcgtcc aggggctggt tgcgcaagcg
     3841 cgagaggatg acgatgagcg tttcctggca cagctcctga gcctgggcga agttgccggt
     3901 catgccgatg atgatctggg tgatgccggg cgcgatgcgc cgcgacaacg cgtcttcagc
     3961 ttttgtgtcg cccgcacgaa tgcggtgcga gaggtcgttg aaggaaagct caggggtttg
     4021 cacgagcgca tcgtggccga acggcaaatg cgcgtaaagg tgtggatgac gctgtatcac
     4081 accaggtcaa actaataaga agcggaccat gcgtgcagcc gccaggctgg tacaggctcc
     4141 gcaggtgtta caccacgcgt ccgcgcctgc caccgcgaag aaagtcctca acgttcaacg
     4201 ccagctcatc caccgcgcgc tgccgcgcct cgaacgcagc ccacgcgata tgcggcgtga
     4261 ccagcagatt cggtatgcca gcctcgaaca acacgctgcc ttccgtcggc ggctctttcg
     4321 gcagcacgtc gatggccgcg ccgcccaggt caccgccctt gagcgcttcg accagcgcct
     4381 cgagatcgac gagcgcaccg cgcgccgtat tgatgagcaa tgcatccggc ttcatcaacg
     4441 cgagctcgcg cttgccgatc aaccccgtcg tcgcgggtgt aagcgggcaa tgcagcgaaa
     4501 gcacgtcggc ggtgcgcagc agttcatcga gcgtgacgcg cacgggcgaa ctctgcgtcg
     4561 tggaatcctc ggacgcggga ccctgtcgtt gcgccacgag cacccgcatg ccgagcgcgt
     4621 cgcgcgcggc attggcaacc gcctttccca gcgtgccgta gccgacgatc ccgagaacct
     4681 tgccggacaa ctcgcgaatg gccgcgcccg gcacggtgaa ttgctcggag cgcgaccagg
     4741 tgccgtcgat ggcagcctgt ccgtactcgc ggaagcgatg cgtgagcagc aggatcgcgc
     4801 cgagcacgtg ctgaaccacg gatacggtgc agtactcgcg gatattgcag acggcgatgc
     4861 ccagctcgcg agcagcttcg agatcgatgt tgttggtgcc ggtggcggtc aatccgatga
     4921 agcgcaggcc cggcacggcg cgcatgcgcg cgcgcgtgat gttcagcttg ttggtgagga
     4981 tgaactcgac gccggcgata cgctcgtcga cttcggcgtc cgtggacgtg gcgtgcagcg
     5041 tgatgcccgg aagcactctc tcgagctgcg tggtgtcgag atcgtcgtgg ctgacagttc
     5101 cgaaatccag gaataccgat ctcatccaga cctctagcta gtcctccatc cgggtggctg
     5161 gcgacggcgc caggcccgcg ggggctgcga gagtaccacc gctgtgggag gtcaccgcaa
     5221 tggctggagg gatcaacttg tacatcacgg gggtgacgag gcgcgccagc agcgtggagg
     5281 tgatcaaccc gccgatgatc acccaggaca tgggcgagta gagcccgatg ttctgcagcg
     5341 ccagcggcag cagtccgccg atggcggtgg ccgaagtgag caggataggc aggaagcgga
     5401 tttcgccggc ctgctcgatg gcctcgtcga gcgccacgcc ttgcgcgcgc agctggttgg
     5461 tgaagtcgac gagcaggatg gagttcttga tctcgatgcc caccagcgcg acgaagccga
     5521 tcgacgcgac gaacgagatg tcgttgcccg acagcagcag catgagcagg ccgccgaata
     5581 caccgagcgg cacgacggtg agcacgatca gcgtggattt gaagttgccg aactcgagca
     5641 cgaggatcgc gaagatgccg aagatggcga cgataattgc gatgccgatg ccgccgaagg
     5701 cttcctgcga ggcctcgtct tcgccaccga ggtcgtaggt gtagccgcgc ggccaatcca
     5761 tctcgttgag cttgtccgtg atctgcgcgg tgagctctcc gatgttgtag ccgggcctcg
     5821 cgtccgcatc gatggtcatg gcgcgttcgc gattgaagcg ctggatcagc gtgggcgcct
     5881 tctcgaactc gagcgaggcg agttgcgaca gcggcagcgt ggtgccgccc agcgaaggca
     5941 cgcgcacctg ctcgagcgca gcgaactcgg cctgctgcgt gatgggtgtg cgcacgacga
     6001 tcggatactg ctcgccggtg gtgtccttga acgtgccgac gctgactccg gcgaccgcga
     6061 ggcgcaccgc gcgatcgagc tccgcggtct gcacgcccag ctgctgcgct ttgcgtgaat
     6121 cgatgcgcag cttgagattg gtgcgcgaca cgttgagcgg attcttcacg tcccgcgtgc
     6181 cgggaatcgc cttgacgaga tcttcgacgc gatggctcaa cgcttcgatg accgcgagat
     6241 cgctgccgat cacgcgcacc gaaatcggcg ccgagatcgg cggcccgttc tggaattcct
     6301 tcacatagat gcgcgcgccg gggtaacgct cgagcttctc gcgcaacagt tgcaactgca
     6361 ccggtgtctc gtcggtgtcg tattcgtgaa gctgcacgaa cacttcggca tagttggccg
     6421 cgtcattgcg ctggatgtgg ttgtagtaga tctgcggatt gccatggccc aggttcgcga
     6481 accagccttt gacttcgggc atcttcgcga gctcggcttc gaccacgtgc agcgcgcgat
     6541 cggtttcgga caacgaactg ccgttcggcg attcgacctg gatcaggaac tgcggcgtgt
     6601 ccgccttggg aaacaggctg gacccgatca ccggcaccag cgccgccgac agcagcagcg
     6661 atccgccgat ggcggcgacg acggtgaact tcgggcgcgc caggcaccag tgcaacgcgg
     6721 gccggtagta gtggtgaatg acgcccatca cgcgtttcag gaaagggttc tcctcgcctt
     6781 ccttgtcttc cttgagcacg cggctcgcga cgaacgggat gatgaacaac gcgagcagca
     6841 gcgatccgat gatggtggcc accacggcca tgggcagcac gcggatgaac tttccggcgg
     6901 ttccgggcag cgccagcaag ggcaggaacg cgaagatgag cgtggccgtg catccgagga
     6961 tggccacgaa tatctggcgc gtgccgtcga gcgcggcctg cacgcgcgac ttgcccatgc
     7021 gcatgtggcg cgcgatgttc tcgacgacga cgatggaatc gtcgaccagg agtccgagcg
     7081 cgaccacgaa gccggcgatc gacaactggt tcagcgagta gtcgaggaag taaaggcagg
     7141 cgaggccgaa agacagcgac agcggaatgg acaccatgac gatgcctgcg gcgcgaaagc
     7201 cgagcggcag caacgtgagc agcaccagcg cgatagcgat gccgaaatcg aaatacagcc
     7261 ggttgagccg tgagcgcacg ttgtcggact ggtcgaatcc cacttcgagc ttgatgcgct
     7321 tcggcaggtc ggccgcgaac tggttagccg ccgccatgac gcgctcgcgc acgtcgagga
     7381 tgttgtagcc gtccttctgg ttggcggtga cgaacaccgc acgtttgccc ttgtagcggc
     7441 cggtgtagct gtacgcggcg gtgctccatg tcacgtcggc cacgtcgctc acgcggacga
     7501 tgcgcccctc tatcgaggcg atgacggtgt cgcgcacctg gtcgagcgac tcgtaacttc
     7561 ccgaggtctt cagcgaaaag ctgcgcgacc ccaggtcgac gaagcccgcg ggaatgttcg
     7621 cgttctcgct ctgcaccgcg ccgatgagct gcgcgggcga gaggttgagc tcggacatgc
     7681 gccgcagatc cacggccacg cgcagctcgc gctccgggta cgcccagctt tccgaagtgc
     7741 gtactccctc gacggtcttg agcttgtcct tgagctcgcg cgcgtaatcc tcgagttcgc
     7801 gatacggcgc gccgtcggac agcagggcga tctgcacggt gttgaccagc ccgggagaaa
     7861 acttgcgcac gatgatctgc acgtcgggcg ggaattccgg acgcaacgca ttcagctcgc
     7921 gcgtgacctc gtcgtatttc ttgtccgcgt cggtggacgc caggaattca atcgccgtga
     7981 acgacacacc gtcgcgaatc gacgtctcca tcttgagcac gtcgtcgagc gcggcgaaac
     8041 ggtcctccag cggtttggcg atcaggcgct cgaggtcctt gggatccgcg cccgggtaga
     8101 tagcggccac ggtgaagccg ggaatcttga aatacgggtc ttcctcgcgc ggcacgctgg
     8161 cgaatgcgta ccagccgagt gcgaccaggc acaggaatgc caccatcgtg aactggtagc
     8221 ggcggatcgg aaactcgagg aacgacatat cagcctcccg ctgctcagcc ttccgtcgtc
     8281 gtgggcggtg cgttcaccgc ctgctgggtc gtgtcacgca gcacctcgat cgcctcgccg
     8341 ttctcgagga acaacgcgcc atcggtgacc acgacttctc cggtcgcaag cccgctctcg
     8401 agcgcgatgc tgtcggcggt gatgaaggcc acgcgcacgt cgcggcgctg cgccttgccg
     8461 ccgtcgacga cgaacacgct ggcgcgatcg ccgtcgcctt cgaccagcgc ggccatcggc
     8521 acgtaggtga gcggcggcgc gtcggtggtg ggcgcgagac gcaggcgcgt gaccaggccg
     8581 ctcaccagcc gtggcggcgg cgaatcgaac tgcacttcga tgggaaacat gccggtgcgt
     8641 tcgtccgcgg cgctcgagac ctcgacgatc gtgccggtca tcggctggcc ggggaaggca
     8701 tccatgcgga tctcgccctt gtcgccgatc ttcacgtt
//
LOCUS       DATDEU010000062         2335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1032034, whole genome shotgun sequence.
ACCESSION   DATDEU010000062 DATDEU010000000
VERSION     DATDEU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032034"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VIV63_01425"
     CDS             <1..624
                     /locus_tag="VIV63_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123282.1"
                     /translation="SEFDVLPLPESLDWLWMFIYCLPAAWLAVDVHRFLLRDLPDARV
                     LVGSGNAKRVLLYALVAFAFWALVIGAVYSGVLDLPAEDSPEMENWMAQMLEGMVIVF
                     VLAVISALLGGRLCLVLPAIALGGDVRAALRAARGNTLRLTVVIALLPVVLAFLIEVI
                     VRGDSTILDQALVVVLGAVVMVVEVSALSLSYRELTQPAPPPTDPPA"
     gene            complement(584..1297)
                     /locus_tag="VIV63_01430"
     CDS             complement(584..1297)
                     /locus_tag="VIV63_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY6123283.1"
                     /translation="MFEASESIVNRAGHLRLTIARRWSRVLAATVFPPECCLCGFPGA
                     SLDLDLCGVCRDDLPWLRNSWCAERSSALVAFNYAQPVDDLIRDLKYRGLTPNARVLG
                     VLLAQAVVERASTLPRLLVPVPLHDRRLRERGFNQAASLARYAGRMLGIPFAARAVRR
                     VRDTPSQTSLTEEERHRNVRAAFAIAGPRHQKRLSGAGHVAVVDDVMTTGSTLNEMKS
                     VLVAAGVRQVDLWAVARVA"
     gene            1373..>2335
                     /gene="bioB"
                     /locus_tag="VIV63_01435"
     CDS             1373..>2335
                     /gene="bioB"
                     /locus_tag="VIV63_01435"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY6123284.1"
                     /translation="MTALRHDWTIAEVEALFALPFADLMFRAQETHRANHQANAVQMS
                     TLLSIKTGACPEDCAYCPQSVRYDTGLEREKLVPLDEVRSRALAAKEAGATRFCMGAA
                     WRSPKKRDVEAVSAMIREVRALGMETCATLGMLTPDQAHELKDAGLDYYNHNVDTSPE
                     FYGEIITTRTYQDRLDTLAAVRDAGLNVCCGGIVGMGETVRDRASMLATLANLPQHPE
                     SVPINQLVRVPGTPLAGKEPVDPFDFVRTIAVARVVMPKAFVRLSAGREAMADELQAL
                     CFLAGANSIFYGEKLLTTGNPDVARDRALMARLGIEPLVPPSVAA"
BASE COUNT          400 a          765 c          738 g          432 t
ORIGIN      
        1 tccgagttcg atgtgctccc attgcccgaa tcgctcgatt ggctgtggat gttcatctac
       61 tgcctgccag ccgcgtggct cgccgtggat gtacaccggt ttctgctgcg cgatcttccg
      121 gatgctcgtg ttctagtcgg atcgggtaat gcaaagcgcg tgttgctata tgcgctcgtg
      181 gcattcgcct tctgggcact ggtcattggc gcggtttatt ccggcgttct ggacttgccg
      241 gcggaagact ctccggagat ggagaactgg atggcgcaga tgcttgaggg catggtcatt
      301 gttttcgtgc tggcggtgat ttccgccttg ttaggcggcc gcctgtgtct cgttctgccc
      361 gccattgcgc tgggcggaga tgtccgggcc gcgctgcgcg cggcgcgtgg caacacgctg
      421 cgcctgaccg tggtcatcgc gttgcttccg gtcgtcctcg catttctgat cgaggtgatc
      481 gttcgcggcg attcgaccat tctggaccag gcgttggtgg tggtgctcgg agcggtcgtc
      541 atggtcgtgg aagtttcggc gctctcgctg tcgtatcgcg aactcacgca acccgcgcca
      601 ccgcccacag atccacctgc ctgacgccgg ccgcgaccag cacgcttttc atttcattga
      661 gcgtgctgcc cgtggtcatg acgtcgtcga ccacggccac gtgacccgcg cctgacaatc
      721 ttttctgatg ccgcggaccg gcgatcgcga acgcggcgcg cacgttgcgg tgccgctcct
      781 cctccgtcag cgaagtttgc gatggcgtat cgcgcacacg cctgacggcg cgggccgcga
      841 acggaatgcc gagcatgcga cccgcgtaac gcgcgaggct tgccgcctgg ttgaagccgc
      901 gttcccgtag acgccggtcg tgcaacggca ccggcacgag aagacgagga agagtcgacg
      961 cccgttcaac gaccgcctgg gccagcaaca cgccgagtac ccgcgcgttc ggcgtgagcc
     1021 cgcgatattt gagatcgcgg atgaggtcgt cgacgggttg cgcgtagttg aacgcgacca
     1081 atgcggacga gcgctcggcg caccacgaat tacgaagcca cggcaggtcg tctcgacaga
     1141 cgccacacaa gtcgaggtcg agcgacgcgc ccggaaaccc gcacaggcag cactcgggcg
     1201 gaaacacggt agccgccagc accctcgacc agcggcgcgc gatcgtcaac cgcaggtggc
     1261 ctgcccggtt gacaatggat tcggacgctt cgaacatgcc tgccagtttc gacgcggttc
     1321 ttccggactg ccagccgaaa acttaacgca atcaacgact aaagggattg gaatgaccgc
     1381 gctacgccac gactggacga tcgccgaagt cgaagccctg tttgccctgc cattcgccga
     1441 tctcatgttc cgcgcccagg aaacccatcg cgcgaaccac caggcgaacg cggtgcagat
     1501 gagcactttg ctgtcgatca agaccggcgc gtgccccgaa gactgcgcct actgcccgca
     1561 atcggtccgt tacgacaccg gcctcgagcg cgagaagctc gtaccgctcg acgaagtgcg
     1621 gtcgcgcgcg cttgcggcga aggaagccgg cgccacgcga ttctgcatgg gcgctgcctg
     1681 gcgttcgccg aagaagcgcg atgtagaagc cgtgtccgcg atgattcgcg aagtacgtgc
     1741 gcttggcatg gagacctgcg cgacgcttgg aatgctcacg ccggatcagg cgcatgagct
     1801 caaagacgcc ggcctcgact actacaacca caacgtcgat acctcgcccg agttctacgg
     1861 cgaaatcatc acgacgcgca cgtaccagga tcgtctcgat acgctggccg cggtgcgcga
     1921 cgccggactc aacgtgtgtt gcggcggcat cgtcggcatg ggcgaaaccg ttcgcgaccg
     1981 cgcctcgatg ctcgcgacgc tggccaacct gccgcagcat ccggaaagcg tgccgatcaa
     2041 ccaactcgtg cgcgtacccg gcacgccgct cgccggcaaa gaacccgtgg atcctttcga
     2101 cttcgtacgc acgatcgccg tggctcgcgt cgtgatgccg aaggccttcg tccggctgtc
     2161 cgcgggtcgc gaggcgatgg ccgatgagct gcaggcgctg tgtttcctcg ccggcgcgaa
     2221 ctccatcttc tacggcgaga aactgctgac cacgggcaat cccgacgtgg cacgcgatcg
     2281 cgccctgatg gcacgcctcg gaatcgaacc tctcgtgccg ccgtccgtcg ccgcg
//
LOCUS       DATDEU010000063         3554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_449246, whole genome shotgun sequence.
ACCESSION   DATDEU010000063 DATDEU010000000
VERSION     DATDEU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449246"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /gene="cysC"
                     /locus_tag="VIV63_01440"
     CDS             <1..1488
                     /gene="cysC"
                     /locus_tag="VIV63_01440"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY6123285.1"
                     /translation="THIVSLLGVQNVILAVNKMDRIDYDRARFEAIAGEFVAYARKLG
                     VQNIEVIPVAALVGVNVVTRGTNETPWYTGRTLLEALEGIEAGRPDPLKELRFPIQLV
                     SRPDANFRGYAGTIASGEIAVGDTVLALPSRKSSKVREITTADGPLERAGTYQAVTVV
                     LEDEIDLSRGDVLVHPDHRPTLARTFDANLIWMGEAPLLPGKRYEFKIGSRYVKGLVE
                     SIQHRIDVNDLTTRAAEALDLNGLARVRIALEEPVAVDDYKLSRATGSFIIVDLLHFG
                     TVGAGMVMAPNAAGQRASSDVVWQPTRVTAAIRANQKSQKPAVVWFTGLSGSGKSTIA
                     NALEQALVQRGHHSYLLDGDNVRHGLNKDLDFSEAGRAENIRRIGEVSALFVDAGLIV
                     ITAFISPYRADRNKIRERIGDNFVEVYLSTSLEECERRDPKGLYVKARAGEIREFTGI
                     DSPYEPPLAPQLTVDTSKQDIGATVDQILRYLENKGFLHQADPGL"
     gene            1485..3371
                     /locus_tag="VIV63_01445"
     CDS             1485..3371
                     /locus_tag="VIV63_01445"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEY6123286.1"
                     /translation="MSGGVVIQGNFPIPEERRDQLQQLATELNREQLMWLSGYFAGAA
                     AAAPSSIPGFQNNLLGAGQARAQLASAAAPAAAPAPAAAAVAAPSLTIISASHTGNGR
                     KISDKLLAAVQALGMQARVVKAGDYQPREIAKEKLLYVIISTHGDGDPPDEARGLYEF
                     LGTKRAPQLPELQYSVLALGDTSYPKFCEAGRVVDERLAKLGAKRLLPRVDCDVDFEK
                     LAQTWADDALARVREIKDKFAPASGGVATGVAQTAAPAQHVPELTRAQPATAEVLANQ
                     RIVGRGALREVRHVELAFPGLGNLYKPGDAVGIVHRNPEPAIDAVLKATKLDAAATVT
                     HDGKTHTLLEWLRDERELTRIARPLLVQVAKHANVDISELLGNAQAMAKLTATCQVSD
                     ILGTYPADWTAEALVNVLRPVTGRVYSIASSPAAVGDEVHLTVAVVGSDTDRKLLAGA
                     ASHFVVHTAVDSNVQVWIEKNERFRVPADGSRDIIMVGPGTGIAPFRGFVQEREATGA
                     TGKNWLFFGGRSLYHDFLYQLEWQQALKRKALHRVDVAFSRDQAEKIYVQHRIRQAGK
                     DLFAWLSNGAYFYVCGDASAMAPDVNAALLDVVREHGNMDADDAQAWLADLTADGRYL
                     RDVY"
     gene            3361..>3554
                     /locus_tag="VIV63_01450"
     CDS             3361..>3554
                     /locus_tag="VIV63_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123287.1"
                     /translation="MSTEVLDRPAAPAKVDPPSPVEIIKKASRGLRGTLVESLADPLT
                     GAIRDADTQLIKFHGSYMQD"
BASE COUNT          625 a         1231 c         1130 g          568 t
ORIGIN      
        1 acccacatcg tctcgttgtt aggcgtgcag aacgtgatcc tcgcggtcaa caagatggac
       61 cgcatcgact acgaccgcgc caggttcgaa gccatcgccg gcgagttcgt cgcgtatgcg
      121 cgcaagctcg gcgtgcagaa catcgaagtg attccggtcg cggcgctggt cggcgtgaac
      181 gtcgtcaccc gtggcacgaa cgaaacgccg tggtacaccg gccgcacgct gctcgaagcg
      241 ctcgaaggca tcgaagcggg ccgcccggat ccgctcaagg aactgcgttt cccgatccag
      301 ctcgtcagcc gcccggatgc gaacttccgc ggctacgccg gcaccatcgc cagtggcgag
      361 atcgccgtgg gcgatacggt gctcgcgctg ccttcgcgca agtcgagcaa ggtgcgcgag
      421 atcaccaccg ccgacggccc gctcgaacgc gccggcacgt accaggcggt cacggttgtc
      481 ctcgaagacg aaatcgacct gtcgcgcggc gacgtgctcg tgcacccgga tcatcgtccg
      541 acgctggcgc gcacgttcga cgcgaacctc atctggatgg gcgaagctcc gctgctgccg
      601 ggcaagcgct atgaattcaa gatcggcagc cgctacgtga agggcctcgt cgagtcgatc
      661 cagcatcgca tcgacgtcaa cgatctcact acccgcgccg ccgaggcgct cgatctcaac
      721 ggcctcgcgc gcgtgcgtat cgcgctcgaa gagcccgtcg ccgtcgatga ctacaagctg
      781 agccgcgcca cgggctcgtt catcatcgtc gacctgctgc acttcggcac cgtcggcgcc
      841 ggcatggtca tggcgccgaa cgcggccggc cagcgcgcca gctccgacgt ggtgtggcag
      901 ccgacgcgcg tcaccgcggc gatccgtgcc aatcagaaga gccagaagcc cgcggtggtc
      961 tggttcacgg gactttcggg ctcgggcaaa tcgacgattg ccaatgcgct cgaacaggcg
     1021 ctggtgcagc gtggccatca cagttatctg ctggacggcg acaacgtgcg ccatggcctc
     1081 aacaaggatc tcgatttctc cgaagccgga cgtgccgaga acatccgccg catcggtgaa
     1141 gtgtcggcgc tgttcgtcga cgccgggctc atcgtgatca ccgccttcat ttcgccttac
     1201 cgcgccgacc gcaacaagat ccgcgagcgc atcggtgaca atttcgtcga ggtctatctg
     1261 tctacttcac tggaagagtg cgagcgccgc gatcccaagg gcctgtacgt caaggcacgc
     1321 gccggcgaga tccgcgagtt caccggcatc gattcgccgt acgagccgcc gctggctccg
     1381 cagctcaccg tcgacacttc gaagcaggac atcggcgcca ccgtggacca gatcctgcgc
     1441 tacctcgaaa acaagggctt cctccaccag gcggatccgg gcctatgagc ggcggtgtag
     1501 tcattcaggg caacttcccg attcccgaag aacgccggga tcagctgcag cagctcgcca
     1561 ccgagctgaa ccgcgagcag ctcatgtggc tgtcgggcta tttcgccggc gccgcggcgg
     1621 ccgcgccgtc gagcattccc gggttccaga acaatctgct gggagcaggc caggcgcgtg
     1681 cgcagctcgc ttccgccgcc gcgcccgcag cagcgccggc accggctgcc gccgccgtcg
     1741 cggcgccttc gctcaccatc atctccgcct cgcacaccgg taacggccgc aagatctccg
     1801 acaagttgtt agccgccgtg caggctctcg gcatgcaggc gcgcgtggtc aaggccggcg
     1861 actaccagcc gcgcgagatc gcgaaagaga agttgctgta cgtcatcatc agcacgcatg
     1921 gcgacggcga tccgccggat gaagcccgcg gcctctacga attcctgggc accaagcgtg
     1981 cgccgcagct ccccgagctc cagtactccg tgctcgcgct cggcgacacc agctatccga
     2041 agttctgcga agccggccgc gtggtcgatg agcgcctcgc gaagctcggc gccaagcggc
     2101 tgctgccgcg cgtcgattgc gacgtcgact tcgagaaact cgcgcagacc tgggccgatg
     2161 acgcgctcgc acgcgtgcgc gagatcaagg acaagttcgc gcccgcctcg ggcggcgttg
     2221 ccaccggtgt cgcgcagaca gccgcgccgg cgcagcacgt tcccgagctc acgcgcgcgc
     2281 agcctgcgac cgccgaagtg ctggccaacc agcgcatcgt gggccgcggc gcgttgcgcg
     2341 aagtgcgcca cgtggaactc gcgttccccg gtctcggcaa tctctacaag cctggcgacg
     2401 ccgtgggcat cgtgcatcgc aatccggagc cggcgatcga tgccgtgttg aaagccacca
     2461 agctcgatgc ggctgccacg gttacacacg acggcaagac ccacacgctg ctcgaatggc
     2521 tgcgcgatga gcgcgagctc acgcgtatcg cgcgtccgct gctggtccag gtcgccaaac
     2581 acgcgaacgt ggatatctcc gagctcctgg gcaacgcgca ggcaatggcc aagctcacgg
     2641 cgacttgcca ggtgtccgac atcctcggca cctacccggc ggactggact gccgaagcgc
     2701 tggtgaacgt gctgcgtccc gtgacgggcc gcgtgtattc gattgcgtcg agccccgctg
     2761 cggtgggcga cgaggtgcat ctcaccgtcg ccgtcgtcgg cagcgatacc gaccgcaaac
     2821 tactcgccgg cgccgcctcg cacttcgtgg tccacaccgc cgtggattcg aacgtgcagg
     2881 tgtggatcga gaagaacgaa cgcttccgcg ttccggccga cggctcgcgc gacatcatca
     2941 tggtgggacc gggcacgggc atcgcgccgt tccgcggatt cgtgcaggaa cgcgaggcca
     3001 cgggcgccac cggcaagaac tggctgttct tcggcggccg ctcgctgtat cacgacttcc
     3061 tttaccagct cgagtggcag caggccctca agcgcaaggc gctgcatcgc gtggacgtgg
     3121 cgttctcgcg tgaccaggcc gaaaagatct acgtgcagca ccgcatccgg caggccggca
     3181 aggatctctt tgcctggctg tcgaacggcg cctatttcta cgtgtgcggt gacgcttccg
     3241 ccatggcccc cgatgtgaac gccgcgttgc tggacgtcgt gcgcgagcac ggcaacatgg
     3301 atgcggacga cgcccaggcc tggctggccg acctcaccgc tgatggacgg tatctacgcg
     3361 atgtctactg aagtactcga tcggccggct gccccggcaa aagtggatcc gccgtcgccg
     3421 gtcgagatca tcaagaaggc gagccgcggc ctgcgcggca cgctggtcga aagtctcgcg
     3481 gatccgctga ccggcgccat ccgcgacgcc gacacgcagc tcatcaagtt ccacggcagc
     3541 tacatgcagg acga
//
LOCUS       DATDEU010000064         7254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2054033, whole genome shotgun sequence.
ACCESSION   DATDEU010000064 DATDEU010000000
VERSION     DATDEU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054033"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..804
                     /locus_tag="VIV63_01455"
     CDS             94..804
                     /locus_tag="VIV63_01455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6123288.1"
                     /translation="MREQYIEYRDGEALLEGFLCYDESQPGPRPAVLISHAWGGRNDF
                     VERKARRLAWQGYACFALDNYGKGKRGTTPEECSALMTPLMKDRKLLLTRLQAGLATA
                     KSMPIVDSRRVAIMGFCFGGLCALDLARSNADIRAAVSFHGMLKPSGLTEPKVRARVL
                     ILHGYDDPLAPPEDVLAVAKEFTDAGADWQLHAYGHTVHSFTNPVAQDRAGGMQYDES
                     ADRRSWHALEEFLGETLK"
     gene            877..1587
                     /locus_tag="VIV63_01460"
     CDS             877..1587
                     /locus_tag="VIV63_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6123289.1"
                     /translation="MKKISVILLVVLSVAFAGFAEAAKPKKRTRNANRVGPYAGALIG
                     YGTYSGDQSDVEAALRQTLINTGAGIENLTSSSEDSDIGYQAVFGYRFIRYFSAELGL
                     AQFGTLSSTAKADMDFGDGFVPTSVKLSFSTGGPMMSVIGILPLGNKFELFGRLGYLF
                     TSSEREFTSKIDGESGSFGSSKGDSQDPVYGVGFAWHINQMYSIRGEFQQLDSLGEAS
                     RSGEEDLTVAGIGLVVRF"
     gene            1587..2744
                     /locus_tag="VIV63_01465"
     CDS             1587..2744
                     /locus_tag="VIV63_01465"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY6123290.1"
                     /translation="MNTRHLLSAGAFSLLVTLSAACSGTDASSATPAAAVRIGNAFVA
                     TPVASFEEPWAMTFLPDGRLLVTEKKGALKLLNVDTRKPGEITGVPKVAYGGQGGFGD
                     VILHPAYASNSFVYVSYSEPGDSGTNGAAVARARLALDNNGGGSLQDFQVIWRQVPKV
                     DSRGHWGHRMAFGPDGKLWITSGERQKFTPSQDMKSNLGKVVRLNDDGSVPKDNPFFA
                     QGGVTAQIWSLGHRNPLGIAFAPDGRLYEHEMGPSGGDELNIIEKGANYGYPIVSNGN
                     HYNGSDIPDHSTRPEFRTPLVTWNPVISPAGFIIYTGKMFPDWTGNGFIGGLSSQSLV
                     RVEFGNGTAREAQRFDMGRRIREVEQGPDGAIWLLEDERNLTGGRLLKLTP"
     gene            complement(2788..3987)
                     /locus_tag="VIV63_01470"
     CDS             complement(2788..3987)
                     /locus_tag="VIV63_01470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6123291.1"
                     /translation="MRRLANKISLAMEERKRLRRRSTMSIAIADRFSQLSAEAWRAAT
                     DGGSVFHSAAYQRMFERVRPPNVEPRYALISDGDAPVAAVCLQIVTLDHSNLGDLRRS
                     RLLRRLGAKMRTRVLVCGNLLVYGLHGVSIAAGADRAKVWQAVSEVIYRVRRAEKLAG
                     STDIVLLKDFDDAARKDSAILNKLSYGAVETEPNMVLTVNPAWRHHDDYLQSLSSKYR
                     SDIKNRVFKKFQESGASIERLDDVEAHAAELQQLYLQVHGNAGLRPFTVPASYWPELA
                     TLSGAEARVHVARRADRIVGFIMSVRDGETALAYHIGFDRQTAGEGVPVYLRLLHASL
                     AQAIDYGARRVSFGRTALEPKARIGCKPETTIVWARHRHPFLNQLLQPMLNLVEHDDA
                     PEFTPFK"
     gene            complement(4003..4686)
                     /locus_tag="VIV63_01475"
     CDS             complement(4003..4686)
                     /locus_tag="VIV63_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY6123292.1"
                     /translation="MCGRYILAPQVKAEKALGVQLVRWNDIRNVNVAATHEVPVVRVA
                     SAADGGGREGVMMRWGLIPPFLKGEDPKFPTINARIETMEASPAFRGAWEKGQRCIAP
                     AQGFYEWEMVPDAPRQPYFVGLAAAEILPFAALWDRSVRADRTVVESFTIITVPANDK
                     RIPAILRMEDVETWLSGTPEQAKSVLTQFPAERLVTRKVSTRVSAPAKHGNDDARLEA
                     VIDSIIEAL"
     gene            4769..5485
                     /locus_tag="VIV63_01480"
     CDS             4769..5485
                     /locus_tag="VIV63_01480"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY6123293.1"
                     /translation="MGRRSDKPDPRVAEALAWLEKHSKKSVRDGMARYAIPNDKALGV
                     LMGDIQKLAKLLGRDHALALALWKTDVYEARMLCAYVDEPERVTSAQMDAQARDFDNW
                     AVCDTLCFALWVQTPHAFKKIKQWANHRDEYVKRASFALLASMALKHKESTDADYLPL
                     LPLMEKAATDERNFVKKAVSWALRGVGRRSARLNKASLALAKKLAASKDAAPRWIGKD
                     AFRELSSAAVQKRLGKKATA"
     gene            complement(5525..5983)
                     /gene="aac(6')"
                     /locus_tag="VIV63_01485"
     CDS             complement(5525..5983)
                     /gene="aac(6')"
                     /locus_tag="VIV63_01485"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: protein motif:HMM:NF043067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HEY6123294.1"
                     /translation="MPLKAPFIERCSSLDQPGWLDMRVMLWPDATADEHRGYMAISLA
                     QPERFLQLMMYDEKRQPIGFIEGSIRSDYVNGTETSPVGFVEGVYVVPSLRRKGIARQ
                     LFAAIGDWAKARGCRELASDALLENESSQRAHRALGFKETERVVYFSKTL"
     gene            6371..6742
                     /locus_tag="VIV63_01490"
     CDS             6371..6742
                     /locus_tag="VIV63_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123295.1"
                     /translation="MIDTAPNIQTKNARLGFAPRGSTLSFLVQTGSGFEAEAILLLGT
                     QTISTWVSSELVGKTATVELVSAGQYQLQITCRFTEKNLTNITIDLEVRAKGATLTSR
                     SKNFTGTAPGTARAIADVYLP"
     gene            complement(6745..>7254)
                     /locus_tag="VIV63_01495"
     CDS             complement(6745..>7254)
                     /locus_tag="VIV63_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123296.1"
                     /translation="VGFASGTPVAVLTLAKLPTGVTSTTARAPGRRMHNTRMGEGARR
                     QEATFKTGSATFRYAGREYTLPLDVAGRHSVTPGLYSYENGVRTALLEYGAGDDQYLL
                     LRVPVASSNEPASLVPYLVLNGRIVPPIDIGACSISVEKVQRTGTAGDVDCGDAPRAV
                     LSKLRFTAE"
BASE COUNT         1357 a         2367 c         2260 g         1270 t
ORIGIN      
        1 gctgtatcga tggactgccc gtcccggagc agtaaaccaa tagcggttat tacataatcg
       61 attcatatat atacccaggc agaaggaccg gacatgcgag agcaatacat cgaataccgc
      121 gacggcgaag cgctgctcga aggtttcctc tgctacgacg aatcgcagcc gggaccgcgt
      181 cccgcggtac tcatctcgca tgcctggggc ggccgcaacg acttcgtgga acgcaaggcg
      241 cggcggctgg cctggcaggg ttatgcgtgt ttcgcgctcg acaactacgg caagggcaaa
      301 cgcggcacga cacccgagga atgcagcgcg ctcatgacgc ccttgatgaa agaccggaaa
      361 ctcctgctga cacggctgca ggccggcctc gccaccgcca agtccatgcc catcgtcgat
      421 tcgcgccgcg tcgcgatcat gggtttctgc ttcggcgggc tctgcgcgct cgacctcgcc
      481 cgcagcaacg cggacatccg cgccgccgtg agctttcacg gcatgctgaa accttccggc
      541 ctcacggaac cgaaagtccg cgcgcgggta ctcattctcc atgggtacga cgatcctctg
      601 gcaccgcccg aagacgtgct cgccgtggcg aaggaattca ctgacgccgg cgccgactgg
      661 cagctccacg cctacggaca tacggtgcat tcgttcacca atcccgttgc ccaggatcgc
      721 gccggcggca tgcagtacga cgaaagtgcc gatcgtcgtt catggcatgc gctcgaagaa
      781 ttcctcggcg aaacgctcaa gtaggtggcg ccaggaaaat tggggaatgg ccggtttgcg
      841 tgttagcgtg gtccaggact tcaaggggcg gtctgcatga aaaagatatc ggtcatcctg
      901 ctcgtcgtgc tcagcgtcgc gttcgcaggt ttcgcggaag ccgccaagcc gaagaagcgc
      961 acgcgcaatg caaatcgcgt cgggccttat gccggcgcgc tgatcggata cggcacgtac
     1021 tcgggagacc agagcgatgt cgaagcagct ctgcggcaaa cgctcatcaa caccggcgcc
     1081 ggcatcgaaa acctgacttc gagctcggaa gactccgata tcggttacca ggcggtgttc
     1141 ggctatcgct tcattcgcta cttctccgcc gaactgggac tcgcgcaatt cggaacgctg
     1201 agctccaccg cgaaagccga catggacttc ggcgacggat tcgttccgac gagcgtcaag
     1261 ctttcattca gcaccggcgg accgatgatg tcggtgatcg gcatccttcc gctcggcaac
     1321 aagttcgaac tgttcggccg gctcggctac ctgttcacga gctccgagcg cgaattcacc
     1381 tcgaagatcg acggcgagtc cggctccttc ggcagctcga agggcgattc gcaggacccg
     1441 gtgtacggag tcggcttcgc ctggcacatc aaccagatgt attcgatccg cggcgaattc
     1501 cagcagctcg actcactggg cgaagcgtcg cgcagcggcg aggaagacct gaccgtcgcc
     1561 ggtatcgggc tggtggtccg cttctgatga acacgcgtca tctcctcagc gcgggcgcct
     1621 tttcactgct cgtcacactg agcgcggcgt gcagcggcac cgacgcttcg agtgccacgc
     1681 ccgcggcggc ggtgcgcatc ggcaatgctt tcgtggctac tccggtcgcg agcttcgaag
     1741 aaccctgggc catgactttc ctgcccgacg ggcggctgct ggtcacggaa aagaaaggcg
     1801 cgctcaaact actcaacgtg gacacgcgga agcccggcga gatcaccggc gtaccgaaag
     1861 tcgcctacgg tggccagggt ggcttcggcg atgtgatcct gcaccccgcg tacgccagca
     1921 attccttcgt ctatgtcagc tattccgagc cgggcgactc gggcacgaac ggcgcggcgg
     1981 tggcgcgtgc gcgtctggcg ctcgacaaca atggcggcgg aagtctgcag gacttccagg
     2041 tgatctggcg gcaggtcccg aaggtcgaca gccgcggtca ctggggccac cgcatggcgt
     2101 tcggtcccga tggcaagttg tggatcacct cgggcgaacg ccagaaattc acgccgtccc
     2161 aggacatgaa gagcaacctc ggcaaggtcg tgcggctcaa cgacgacgga tcggtgccga
     2221 aggacaaccc gttcttcgcg caaggcggcg tcaccgcgca gatctggtcg ctgggtcacc
     2281 gcaaccctct cggcatcgcg ttcgcgccgg acggcaggtt gtacgaacac gagatggggc
     2341 caagcggcgg cgacgagctc aacatcatcg agaagggtgc gaattacggt tacccgatcg
     2401 tgtcgaacgg caaccattac aacggctccg acattccgga tcattcgacg cgtcccgagt
     2461 tccggacgcc actcgtcacc tggaatccgg tcatctcgcc cgcgggcttc atcatctaca
     2521 cgggaaagat gtttccggac tggaccggca acggcttcat cgggggcttg agttcgcagt
     2581 cactggtgcg cgtggaattc ggcaacggca ccgcgcgtga ggcgcagcgt ttcgacatgg
     2641 gccgccgcat ccgcgaagtc gagcaaggtc ccgatggcgc catctggctg ctcgaagacg
     2701 aacgtaacct gaccggcggc cgcctcctca aactcacccc gtaaatgggg tcagacccca
     2761 attccgggaa atggggcctg accccattta tttgaacggc gtgaactcgg gcgcgtcgtc
     2821 gtgctcgacg agattcaaca tgggctgcag cagctgattc aggaaaggat ggcggtggcg
     2881 cgcccacacg atcgtggttt caggcttgca gccgatgcgc gccttgggtt cgagcgcggt
     2941 gcggccgaac gagacgcgtc gcgcgccgta gtcgatggct tgcgcgagcg acgcatgcag
     3001 cagccgtaga tagaccggca cgccctcgcc cgccgtctgc cgatcgaaac cgatgtgata
     3061 ggccagtgcc gtctcgccgt cgcgcacgct catgatgaac ccgacgatcc gatccgcgcg
     3121 gcgcgccacg tgcacacgcg cttccgcgcc cgacagcgtg gcaagctccg gccagtagct
     3181 ggccggcacg gtgaacggcc gcagccccgc gttgccatgc acctgcagat agagctgctg
     3241 caactccgcc gcatgcgcct cgacatcatc gagccgctcg atgctggcac cggattcctg
     3301 gaacttcttg aacacgcggt tcttgatgtc gctgcgatac ttggagctca acgactgcag
     3361 atagtcgtcg tggtgccgcc aggcgggatt caccgtcagc accatgttcg gctcggtctc
     3421 caccgcaccg tagctcagct tgttcaggat cgcgctgtcc ttgcgcgccg cgtcgtcgaa
     3481 gtccttgagc agcacgatgt ccgtgctgcc ggcgagtttc tccgcgcggc gcacgcgata
     3541 gatgacctcg ctcaccgcct gccacacctt ggcgcgatcc gctccggccg caatgctcac
     3601 gccgtgcagc ccgtaaacca gcaggttgcc gcacaccagc acgcgcgtgc gcatcttcgc
     3661 gcccagcctg cgcaataggc ggctgcgccg gaggtcgccc aggttcgagt gatcgagcgt
     3721 cacgatctgc aggcacaccg ccgccactgg cgcgtcgccg tcgctgatca gtgcgtagcg
     3781 cggctcgaca ttgggtggcc ggacgcgctc gaacatgcgc tgataggcgg cggagtggaa
     3841 cacgctgccg ccgtcggtcg cggcacgcca ggcttccgcc gacaactgcg agaaacggtc
     3901 cgcgatggcg atggacatgg ttgagcggcg gcgcagccgc ttgcgctctt ccatcgcgag
     3961 cgagatcttg ttagccagtc ggcgcatccg gttacccgtg actcataacg cttcgatgat
     4021 cgaatcgatg accgcctcga ggcgcgcatc gtcgttgccg tgtttcgcgg gcgcgcttac
     4081 gcgcgtgctc accttgcgcg tgacgagccg ctccgcggga aactgcgtga gcaccgactt
     4141 cgcctgctcg ggcgtgccgc tcagccatgt ctccacgtct tccattcgca ggatcgccgg
     4201 tatgcgcttg tcgttcgccg gcaccgtgat gatggtgaag ctttccacga cagtacgatc
     4261 ggccctgacc gaccggtccc acagcgcggc gaacggcagg atttccgcag ctgcgaggcc
     4321 gacaaagtac ggttgtctcg gcgcgtccgg caccatctcc cattcgtaga atccctgcgc
     4381 cggcgcgatg cagcgctgac ccttctccca cgcgccgcga aaagcaggcg acgcttccat
     4441 ggtttcgatg cgggcattga tcgtcgggaa cttcgggtct tcgcctttga gaaaaggcgg
     4501 tatcagtccc catcgcatca tcacgccttc gcgcccgccg ccgtctgcgg ccgaagcgac
     4561 gcgcaccacc gggacttcat gggtcgcggc gacgtttaca ttccggatgt cgttccagcg
     4621 tacgagttgc acgccgaggg ctttttccgc cttcacctgc ggcgcgagga tatagcgtcc
     4681 acacataggg acggcagctt agcggcttga cccgcgcaaa caaatgggga cactccccat
     4741 ttcctagcaa aaggggtcgg agtccaacat gggtagaagg agtgacaaac cagacccgcg
     4801 tgtcgccgag gcgctcgcct ggctcgagaa gcactcgaag aaatccgtgc gcgacggcat
     4861 ggcgcgatac gccattccga acgacaaggc gctcggcgtg ctcatgggcg acatccagaa
     4921 gctggccaag ctgctcggcc gcgatcacgc gcttgcgctc gcactctgga agaccgacgt
     4981 ctacgaagcg cgcatgttgt gcgcgtatgt cgacgagccc gagcgcgtga cgtccgcgca
     5041 aatggacgcc caagcgcgcg acttcgacaa ctgggcggtg tgcgacacgc tgtgttttgc
     5101 gttgtgggtg caaacgccgc acgcgttcaa gaagatcaag cagtgggcga accatcgcga
     5161 tgaatacgtc aagcgcgcat cgttcgcgct gctcgcgagc atggcgctga agcacaagga
     5221 gtcgaccgac gccgactatc ttccgctgct gccgctcatg gagaaagccg ccaccgacga
     5281 acgcaacttc gtgaagaagg cggtgagctg ggcgctgcgc ggtgtggggc gccgaagcgc
     5341 caggctgaac aaggcgtcgc tggcgctcgc gaagaagttg gccgcgtcga aagacgccgc
     5401 gccacggtgg atcggcaagg atgcgtttcg cgagttgtcg agcgccgccg tgcagaagcg
     5461 gctcgggaag aaggcgaccg cttgaggcgc gtcccctttt gctagcaaat caggaatgtc
     5521 cccattaaag cgtcttgctg aagtagacga cgcgctcggt ctccttgaag ccgagcgctc
     5581 gatgcgcgcg ctggctgctc tcgttctcga gcaacgcgtc ggaagcaagc tcacggcatc
     5641 cgcgcgcttt cgcccagtcg ccgatcgccg cgaacaattg gcgcgcgatt cccttccggc
     5701 gcagtgacgg caccacgtac acgccttcga cgaatcccac gggtgaagtc tcagtcccat
     5761 tgacgtagtc gctgcggatc gagccttcga tgaagccgat cggctgacgc ttctcgtcgt
     5821 acatcatgag ctgcaggaat cgctcgggct gggcgagcga aatcgccata tagccgcgat
     5881 gctcgtcggc ggtggcgtcc ggccagagca tcacgcgcat atccagccag ccgggctggt
     5941 cgagcgagct gcaacgttcg atgaaagggg ccttaagggg catgcgagga gcgtatcagg
     6001 ccacccttgg gttcatacag tgccgaggcc cgtttcgaac tgggtggacg tccccacccg
     6061 ggcggcaaaa aatcccaccc atgcctctcc cctccaccgc catcgacaaa ctttcagacc
     6121 actcaggggc ttatgtcatc tgcgtcgcct gccagcggtg ccggcacgag cgcgaaattg
     6181 acccccggac cctagctaac atcctcggct ggaacgtcac gctgaaacag gcctgcaccc
     6241 gcctgcgttg ttcccaatgc caggcccgca ccttcaaggt ggagatcgcc ttcgatcgca
     6301 aaccccgggg atggaaagcc aacccgtcct gagcgaaatg gtttccggcg tcgcggcagt
     6361 aactagtcag gtgatcgata ccgcgcccaa catccagacc aagaacgccc gcctcgggtt
     6421 cgcgccccgg gggtcgacct tgagttttct cgtgcagacg ggatccgggt tcgaggccga
     6481 agccattctc ttgttaggca cgcagaccat ttccacgtgg gtatcgtccg aactcgtggg
     6541 caagacggcc acggtcgagc tggtgtcggc gggccagtac cagctccaga tcacgtgccg
     6601 cttcacggaa aagaatctca cgaacatcac gatcgacctg gaggtccgcg cgaagggcgc
     6661 cacgctcacg tcgaggagca agaacttcac cggcacggca ccgggcactg ctcgcgccat
     6721 cgccgacgtc tacctgccct gatcttattc cgcggtgaat cggagcttcg acaacaccgc
     6781 gcgcggcgca tcgccacaat ccacgtcgcc ggccgttccg gtccgctgca ctttctccac
     6841 gctgattgaa cacgcgccga tgtcgatggg cggcacgatg cgtccgttca gcacgagata
     6901 gggcacgaga ctcgccggct cattactgct ggcgaccggc acgcgcagca gcaggtattg
     6961 gtcgtcaccc gcgccatact cgagcagcgc ggtgcgcacg ccgttctcgt aggaatacaa
     7021 ccccggcgtc accgaatgac gcccggccac gtcgagcggc agcgtgtatt cacggcccgc
     7081 gtaccggaac gtagccgacc cggtcttgaa cgtggcttct tggcggcgcg cgccctcgcc
     7141 catgcgcgtg ttgtgcatgc gccggccggg agctcgtgcg gtggtggagg tgacacccgt
     7201 gggaagtttc gcgagcgtga gcaccgcgac cggcgtcccc gacgcaaacc ccac
//
LOCUS       DATDEU010000065         3198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1907778, whole genome shotgun sequence.
ACCESSION   DATDEU010000065 DATDEU010000000
VERSION     DATDEU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907778"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1837)
                     /locus_tag="VIV63_01500"
     CDS             complement(215..1837)
                     /locus_tag="VIV63_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6123297.1"
                     /translation="MNISRSSIKSSFDVVIVGGGHNGLTCACYLAQSGLSVGVFERRS
                     ILGGAAVTEEFHPGFRNSTASYTVSLLNPKVIKDLRLAEHGLKILERPMQNFLPLPDG
                     RALVAGPDSDRTAASVRSFSARDADRLPAYYKMLDAAVTLLRSQLLETPPANPRRLRD
                     LWGALQMGRSFRKLDASVQRDIHELFTRSAGDLLDHWFESDAVKALYGFDAVVGSYAS
                     PYTPGSAYVLLHHVFGEVNGKPGVWGHAVGGMGAITQAMANEARRLGVTLETDAPVKR
                     IVVERGRATGIELEDGRVIQGNAVAANLGPKPLFLKLLADADLPAEFRARIERLRVGS
                     ATFRMNVALSELPKFLAAPRDDGGAHLGSGIILAPSLEYMDHAFADARLTGMSRKPIV
                     EMLIPSTLDSSLAPKGAHVASLFCQHFSYKLPDGRDWSQQKQSAEDLILSTVESYAPN
                     FRASIVGTSALSPLDLEEKLGLAGGDIFHGALGLDQLWAARPMLGYGDYRTPVKSLYL
                     CGSGAHPGGGVTGLPGHNAAREIMRDGVSPNS"
     gene            1883..2500
                     /gene="lexA"
                     /locus_tag="VIV63_01505"
     CDS             1883..2500
                     /gene="lexA"
                     /locus_tag="VIV63_01505"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY6123298.1"
                     /translation="MSDLTPRQTEILRLIQRAINETGMPPTRAEIAGELGFKSPNAAE
                     EHLRALARKGVIALVPGASRGIKLMDTMREQMGLPLIGRVAAGRPILAEENIESRLDI
                     DPAIFSPKPHYLLKVIGMSMKDAGILDGDLVAVHRTPEVRNRQIIVARLENEVTVKRY
                     KQDGSVVWLLPENADFEPIRVNIKEDPLIIEGVVVGVLRRGKALS"
     gene            2556..2912
                     /locus_tag="VIV63_01510"
     CDS             2556..2912
                     /locus_tag="VIV63_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6123299.1"
                     /translation="MLLGLRTAIYHTPDIAAGKLWYMKVLGFPPYFDQPFYVGFNVAG
                     FELGLTPDAEKSSVGAYWGVKDARSSYARLLELGAEPRGEIQDVGEGILIADVLDPWG
                     NVFGIIENPNFGLSTQ"
     gene            2921..>3198
                     /locus_tag="VIV63_01515"
     CDS             2921..>3198
                     /locus_tag="VIV63_01515"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEY6123300.1"
                     /translation="MKRASGSFEVSIQSLANNEVSADPMLGRFLLTKKFSGDLDASAC
                     GHMLSAGTAIKGSAGYVAIDQVTGSLDGRQGSFILQHSGSMKRGAPTLS"
BASE COUNT          636 a          960 c          971 g          631 t
ORIGIN      
        1 aacgctgcaa cggcagcaac cttgataatc gcactcttca taacgacaaa tccccttatt
       61 ggtggttgag caagtccgct tccccatcaa ctcgcatcga atgcgatctg agtgaaggta
      121 gacgtctatt aggcgggcgc aactgcaaca aattcgcgag ttgtttgcaa ctgaaatttc
      181 tttcgtgtcg gcctgtggcg acaatagcaa gttgtcagct attagggcta acaccatctc
      241 gcattatttc tcgcgccgcg ttgtgtccgg gaagtccggt cacgccaccg ccgggatgtg
      301 caccggaacc gcacaagtag aggcttttca ccggtgtgcg gtaatcgccg taacctaaca
      361 tcggccgcgc agcccacagt tggtcgaggc ctaacgctcc gtggaaaata tcgccgccag
      421 ccaggccgag tttttcttcg agatcgagcg gactcagcgc cgaagtgccg acgatactcg
      481 cgcgaaagtt gggcgcgtac gattccactg tcgacaagat gaggtcttcc gcactctgct
      541 tttgctgact ccagtcgcgg ccgtccggca atttgtagga gaaatgctga cagaacaacg
      601 aggcaacgtg agctcccttt ggcgcgagag aactgtcaag cgtcgaggga atgagcattt
      661 ccacgatggg cttgcgcgac atgccggtga ggcgcgcatc ggcgaatgcg tggtccatgt
      721 attcgagtga cggcgcgaga atgattccgg atccgagatg agcgccgccg tcatcgcgcg
      781 gtgcagcgag gaatttcggc agttcggaca gcgccacgtt catgcggaac gtcgccgatc
      841 ccacgcgcaa tcgctcgatg cgcgcgcgaa actcggcggg cagatccgcg tcggctaaca
      901 gtttgaggaa gagtggtttg gggccgaggt tggcagccac cgcattgccc tggatcacgc
      961 gtccgtcttc gagctcgatc ccggtcgcgc ggccgcgttc gacgacgatg cgttttaccg
     1021 gtgcatcggt ctcgagtgtg acgcccaggc gtcgcgcctc gttggccatg gcttgcgtta
     1081 tcgctcccat tccgccgacg gcgtgtcccc agacgccagg cttgccattc acttcaccga
     1141 acacgtggtg gagaagtacg taggcactgc cgggcgtata ggggctcgcg tagctgccaa
     1201 ctaccgcgtc gaagccgtac agcgccttga cggcgtcgct ctcgaaccag tgatctaaca
     1261 agtcgccggc gctgcgcgtg aacagttcgt ggatgtcgcg ctgcacactg gcatcgagtt
     1321 tgcggaagct gcggcccatc tgcagcgcgc cccacaggtc gcgcagccgg cgcggattcg
     1381 ccggcggagt ttcgagcagc tgcgatcgca gcagcgtgac cgccgcgtcg agcatcttgt
     1441 agtaggcggg caggcgatcc gcatcgcgtg cggagaagct gcgcaccgag gccgcggtgc
     1501 ggtcgctgtc cgggcctgcc acgagggcgc ggccatcggg cagcggcaga aaattctgca
     1561 tgggccgctc gaggattttc aggccgtgtt cggccaggcg cagatccttg atcactttcg
     1621 ggttcagcag gctgacggtg taactcgcgg tggagttgcg gaagcccggg tggaattcct
     1681 cggtgacggc ggcgccgccg agtatcgagc ggcgttcgaa tacaccgacg gagaggcccg
     1741 attgggcgag gtaacacgca caggtgagac cgttgtggcc gccacccacg atgaccacat
     1801 cgaagctgct tttgatgctt gacctggata tattcacagt gtacaaatcc tcagccctga
     1861 ttcttcttta aaggaatccg tcatgtccga cctcacccca cgccagacag aaatcctgcg
     1921 tctcatccag cgtgccatca acgaaaccgg catgcccccc acgcgcgctg aaatcgccgg
     1981 cgagctcggt ttcaaatctc cgaacgccgc cgaagagcat ctccgtgccc tggcgcgcaa
     2041 aggcgtcatt gcgctcgtgc ccggcgcttc gcgcggcatc aagctcatgg acaccatgcg
     2101 cgaacaaatg ggtctgccgc tcatcggccg tgtcgccgcg ggccgcccca tactcgccga
     2161 agagaatatc gaatcccggc tcgatatcga ccccgcaatc ttctcaccca agccgcatta
     2221 cctgctcaag gtcatcggca tgtcgatgaa agacgccggc atcctcgatg gcgacctggt
     2281 ggccgtgcat cgtacgcctg aagtgcgtaa ccggcagatc atcgtggcgc gtctcgaaaa
     2341 cgaagtcacc gtgaagcgct acaaacaaga cggttcggtg gtgtggctgc tgccggagaa
     2401 cgcggacttc gaacccattc gcgtcaacat caaggaagac ccgctcatca tcgaaggcgt
     2461 ggtggtgggt gttctgcggc ggggtaaagc tctttcgtga tccgccgcag actcagataa
     2521 gacgaagacc cagaagacca agagacattc catccatgtt gttaggtttg cgcacggcta
     2581 tttatcacac gccggatatc gccgcgggca agctctggta catgaaggtc ctgggctttc
     2641 cgccgtactt cgaccagcct ttctacgtgg gtttcaatgt cgccggtttc gagctgggcc
     2701 tgacgcccga cgccgaaaaa tcttcggttg gcgcgtactg gggtgtcaaa gacgccaggt
     2761 cctcgtacgc gcggttgctc gaactcggcg ccgaaccgcg tggcgagatc caggacgtag
     2821 gcgagggcat cctcatcgcc gacgtgctcg atccgtgggg aaatgtgttc ggcatcatcg
     2881 aaaacccgaa cttcgggttg tctacccaat aaagatggat atgaaacgcg cctccggaag
     2941 ttttgaagtc agcatccagt cgctggcaaa caatgaagtt tcagccgatc ccatgctggg
     3001 gcgttttctg ctcaccaaga aattcagcgg cgacctcgat gcgtcggcgt gtgggcacat
     3061 gttgtccgcg ggcacggcca tcaagggttc cgcgggttac gtggccatcg accaggtcac
     3121 gggttctctc gatgggcgcc agggcagttt catcctgcag cactcgggaa gcatgaagcg
     3181 cggcgcgcca accctctc
//
LOCUS       DATDEU010000066         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_449402, whole genome shotgun sequence.
ACCESSION   DATDEU010000066 DATDEU010000000
VERSION     DATDEU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449402"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2299)
                     /locus_tag="VIV63_01520"
     CDS             complement(<1..>2299)
                     /locus_tag="VIV63_01520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6123301.1"
                     /translation="GLRATGLLQPLENGVADARARLTMREVRSDIVIVGIDAASLATL
                     DRWPWPRRHHAKLLEEISRAAPARVFMDIDFSSQSNQIDDAVLEAALAKPRDFPILLP
                     TYFQNASGTDESLIVSKPLRRFARSSERVVVNAQSAPDGLIRHWRNSWLVEGARERSV
                     IDPRRVLPDGQDVPIDYSISPASFIYVSYVDVLEGRVPRAALAGKDVYVGATAVELND
                     MLAVPLHRSLPGIVVQALAAESVNEGAPRPAPAWMWVGLLAIWIALAALFYAARAGVK
                     WYRNLAVLTLLMAAITGVALFAAVYDRLLLDTAAPMLAVALLFVAVTVRSLEKQTLRA
                     LSYAVGMRRRDALLKSVVQSSTDCILCIDENGIIKTANPAASRLFGCAMYELLDEPIA
                     KFIALLAGDGAGARLGALHGVIRECDARTLNGEVFPVEISMSRVRLNTERLYTAIVRD
                     IRERRAQQRRLQHQATHDSLTGLPNRAALLSQLESALVPGSGRNSLALLMLDLCRFKE
                     VNDTLGHNVGDRVLCDVAQRFAAALGDRGLIARIGGDEFTVMIEGPESDEEIATTVQL
                     LSDCLRTPIDVVGIAIEVGVSIGVARYPRDSSDAQILLRHADVAMYMAKRRGSAFEWY
                     DAALDENSVRKLAIGGELRAAITSNSLQLNYQPQVNLRTGMVESVEALLRWVHPTQGP
                     INPGEFIAIAEATDLIRPLTEWTLKTALAQARSWRERGVHVRVAVNLSARLLQDTAFP
                     ARLRKLLQDSGVSAASLELEITESAM"
BASE COUNT          392 a          767 c          776 g          364 t
ORIGIN      
        1 tcatcgcgct ttccgtgatc tcgagttcga gcgaggcggc ggagacgccg gaatcctgca
       61 gcagctttct cagacgagcc gggaacgccg tgtcctgcag caaccgtgcg gacagattca
      121 ccgcgacgcg cacgtgcacg ccgcgctcgc gccaggaacg cgcctgcgcg agcgcggtct
      181 tcagcgtcca ctcggtgagc gggcggatga gatcggtggc ctcggcaatc gcgatgaact
      241 cgcccggatt gatcggcccc tgcgtgggat gaacccagcg cagcagcgcc tcgacgctct
      301 cgaccatgcc ggtgcgcaga ttcacctgcg gctggtaatt gagctgaagc gagttgctgg
      361 tgatggcggc gcgcagctcg ccgccgatcg cgagcttgcg cacgctgttt tcgtcgagcg
      421 cggcgtcgta ccactcgaag gccgatcccc ggcgcttggc catgtacatg gcgacgtccg
      481 cgtggcgcaa caggatctga gcatcgctcg aatcgcgcgg atatcgcgcg acgccgatgc
      541 tcacgcccac ttcgatggcg atgccgacga cgtcgatggg cgtacgcaga caatccgaca
      601 ggagctgcac cgtcgtggcg atctcctcgt cgctttccgg accttcgatc atcacggtga
      661 actcgtcgcc gccgatgcgc gcgatgagtc cacggtcgcc gagcgccgcc gcgaatctct
      721 gcgcgacgtc gcacaacacg cggtcgccga cgttgtggcc cagcgtgtcg ttgacctcct
      781 tgaaacggca caagtccagc atgagcagcg ccagtgaatt cctcccggac ccgggcacca
      841 gcgcggattc gagctgcgag agcagcgcgg cgcgattcgg caggccggtc agggaatcgt
      901 gcgtcgcctg gtgctgcagg cggcgctgct gcgcgcggcg ctcgcggatg tcgcgcacga
      961 tggccgtgta cagcctttcc gtgttgagcc gcacccggct catggagatc tccacgggga
     1021 acacttcgcc gttcagcgtg cgggcatcac attcgcggat cacgccgtgc agcgcaccga
     1081 gccgcgcgcc cgcgccatcg ccggccagca aggcgatgaa cttggcgatg ggttcgtcga
     1141 gcagttcgta catcgcgcag ccaaacaggc gcgacgcggc gggattcgcg gtcttgatga
     1201 ttccgttctc gtcgatgcac aggatgcagt cggtcgaaga ctgcacgacg ctcttcagca
     1261 gcgcgtcgcg ccggcgcatg ccgaccgcgt acgacaacgc gcgcaaggtc tgcttctcga
     1321 gtgaccgcac tgtcaccgcg acgaacagca gcgcgacggc gagcatgggc gcggccgtgt
     1381 cgagcagcaa tcgatcgtag acagcggcga acagcgctac gccggtgatg gccgccatca
     1441 acagcgtcag caccgccagg ttccgatacc acttcacgcc ggccctggcg gcgtagaaca
     1501 gcgcggcgag cgcgatccag atcgccagca acccgaccca catccacgcg ggcgcggggc
     1561 gcggggcccc ttcgttcacg ctctcggccg ccagtgcctg cacgacgatg ccgggtagcg
     1621 agcggtgcag gggcaccgcc agcatgtcgt tgagctccac ggcggtcgct ccgacataga
     1681 cgtccttgcc ggcgagcgcc gcacgcggca cgcggccttc caggacatcg acgtaagaca
     1741 cgtagatgaa tgaggcgggc gaaatcgagt aatcgatggg cacgtcctgc ccgtccggca
     1801 gaacgcggcg ggggtcgatg acactgcgct cgcgtgcgcc ttcgaccagc caggaattgc
     1861 gccagtggcg tatgagtcca tcgggcgccg actgggcgtt cacgaccacc cgttccgagc
     1921 tgcgcgcgaa acgccgcaac ggcttgctga cgatcagcga ctcatcggtg ccgctggcat
     1981 tctggaagta ggtaggcaac aggatcggga agtcccgcgg ttttgcgaga gctgcctcca
     2041 gcaccgcgtc gtcgatctgg ttcgactgcg agctgaagtc gatgtccatg aacacacggg
     2101 cgggagcggc gcgcgagatc tcttcgagca gtttcgcgtg atggcggcgc ggccatggcc
     2161 agcggtcgag cgtcgcgagg ctcgccgcgt cgattccgac aatgacgatg tcggagcgaa
     2221 cctcgcgcat cgtcaggcgc gcgcgtgcgt ccgccacccc gttctcgagc ggctggagca
     2281 ggccggtggc gcgaagccc
//
LOCUS       DATDEU010000067         4693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1178655, whole genome shotgun sequence.
ACCESSION   DATDEU010000067 DATDEU010000000
VERSION     DATDEU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178655"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1564
                     /locus_tag="VIV63_01525"
     CDS             266..1564
                     /locus_tag="VIV63_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6123302.1"
                     /translation="MAIQRITQRITIVGAGQAAAQAVETLNKRGQEVSVTLIGDELLL
                     PYQRPPLSKKFLAGSLDRDRLLIRHPAHYVDHGIDLRLGFAAVNIDRERRRVEIADGG
                     SVEYDRLLLATGSHPRLLPLPGAELAGVHYLRSVADVDRLRAELAPGRRAVIIGGGYI
                     GLEVAATCREAGLEVTVLEAADRVMNRVVSPAVSAFYEAEHARHGVEIRCGVRIDSLV
                     GEGEKGDRSIFPSGKIDLTPLSPSPERVAAVRLDDGHELPADFVLVAIGVAPTDALAR
                     AAGLDCDNGILVDEYCRTSDPHIWSAGDCARHPSIHYGTRVRLESVDNAFEQGTSAAL
                     NMLGIATVHDKVPWFWSDQFDLKMVIVGLSAAHDEVVLRGDPASRAFSVCYLRDGELI
                     AVEAINHTKDQMAARKLIPARARPDLARLSDPQIALKETV"
     gene            complement(1548..2729)
                     /gene="tal"
                     /locus_tag="VIV63_01530"
     CDS             complement(1548..2729)
                     /gene="tal"
                     /locus_tag="VIV63_01530"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY6123303.1"
                     /translation="MGNPLEALQLHGQSVWLDYIRRDLITSGELQRLIDDGVRGVTSN
                     PAIFEKAIAGSADYNDVLAAANARNADAKTVYESIAVRDIQDAADVLLPVYERTAKRD
                     GYVSLEVSPKLARDASGTIEEGRRLWKAVARANLMIKVPATPEGIVAIRQLISEAVNV
                     NVTLLFSQEAYAQVVEAYLAGLEDRAANGASLEGIASVASFFISRIDSVVDARIKERD
                     LSRALLGKVAIANARLTYQRYRELFGGPRWDSLAQRGAQTQRVLWASTGTKDPAYRDV
                     LYVEELIGPDTVNTMPPATLAAFRDHGVARASLVEDLEGAAETLLQLEKEGIAIREVT
                     DLLLEQGVQLFSDAFDKLLAAVRQRTGTVLSGMVDLETSEPATADLATGRQESSVRRS
                     P"
     gene            complement(2808..3041)
                     /locus_tag="VIV63_01535"
     CDS             complement(2808..3041)
                     /locus_tag="VIV63_01535"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123304.1"
                     /translation="MAVMNDLDRFHLFGDVIDRLPQLGSRAAYSKQAMNEKLLDHRQY
                     ITEHGDDMPEITGWRWGQRVVKGHGTSTEGDNV"
     gene            complement(3157..>4693)
                     /locus_tag="VIV63_01540"
     CDS             complement(3157..>4693)
                     /locus_tag="VIV63_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123305.1"
                     /translation="MPGAGSVPAHDGFMLIAIAGIALLALYLLATLAFALSTVLPPLR
                     QLSAGQYLRQRLGQLLGGGFTCIGIGLLPVLAGVEETVATYLRVDFAQELTRHLTWIV
                     PLVTGMLSVRQANKQGAHSGTFAVAGLALVLCGLFTALYHLAHHTMLLETPAFMAWLA
                     ASVVFAFVCDINAISMHSYYRSRLAEAFLPEVGAGVDPRHGGADRHPMLFRLADVRAT
                     SGGPFHIINTTLNTTSSSNAKLRARNGENMILTPLYCGSSATGYRRTTNYLRGELTLS
                     TAFSVSGAAVDPDTYATHSRPVSFLMALLNIRLGVWLANPSHEPRRLRWPAWYQLMMR
                     EMFGVGLDEKQARIHLADGGHFENLGLYELLRRRCRYIIVSDAGADPDTTLADLGQAV
                     QRARADLGASVDICADPLIHDGAGLRTQAHLLGRVSYADGSHGEILYLKALLRTNLGA
                     DIYGYWRTNPDFPNQPTSNQFYGEMQFDSYRELGRELMSGIAGEDSNGDIERVFARWR
                     KEA"
BASE COUNT          824 a         1497 c         1512 g          860 t
ORIGIN      
        1 acgtcgtcga gtttgaaagg cttgatgatg gcggtgacca atttcatttc tttatctccc
       61 aattctttag tgaatctgga cgccgacatc ggcgagcctt aatcgcagtg caccttccgt
      121 gccagtatcc cgaaagctca acatagcggc aatttcgtta ttgggcttct ggccagagaa
      181 tttcccggca ctaaattagc gcggtgattc tttatccgcc caacaatggt gcgaccacgt
      241 tcgtgaagta ctctctctta tctatatggc gatccaacga atcacccaac gcatcacgat
      301 agtcggcgct ggacaggcgg cagcgcaagc ggtcgagacg ctgaacaagc gcgggcagga
      361 agtctcggtc acgctgatcg gcgatgaact cctgctgccc tatcaacgcc ccccgctgtc
      421 gaagaagttt ctggcagggt cgctggaccg tgaccgcctg ctgatccgtc atccggctca
      481 ttacgtcgac cacgggatag atctgcggct cggctttgcc gcggtgaaca tcgatcgcga
      541 gcggcgccgt gtcgagattg ccgatggcgg gtcggtcgag tacgaccgcc tgctcctcgc
      601 caccggcagt catccgcgcc tgctgccgtt gcccggcgcc gaactggcgg gggtgcatta
      661 cctgcgcagc gtcgccgatg tggaccggct gcgtgccgaa ctggcgcccg gccggcgcgc
      721 ggtcatcatc ggtggcgggt acatcggcct cgaagtggcc gccacctgcc gcgaggcggg
      781 gctcgaggtc accgtgctcg aggccgcgga ccgcgtgatg aatcgcgtgg tcagcccggc
      841 ggtgtcggca ttttatgagg ccgaacatgc aagacacggc gtcgagatcc ggtgcggcgt
      901 ccgcatcgac tcgctggtgg gagaagggga aaaaggggac agatctattt ttccgtctgg
      961 aaaaatagat ctgacccctt tgtccccttc tcccgagcgg gtggccgctg tccggctcga
     1021 cgatggtcac gaactgcccg ccgacttcgt gctggtagcc atcggcgtcg cgccaacaga
     1081 cgcgctggct cgcgcggcgg gactcgactg cgacaacggc atcctcgtcg atgagtactg
     1141 ccgtacgtcc gatccccaca tctggtcggc aggcgactgc gcgcgccatc cgagcattca
     1201 ctacggcacg cgggtgcggc tggagtccgt cgacaacgcg tttgaacagg gcaccagtgc
     1261 ggcgctcaac atgctcggaa tcgccacggt gcacgacaag gtgccctggt tctggtccga
     1321 tcagttcgat ctcaagatgg tgatcgtggg gctgtccgcg gcgcacgacg aggtggtgct
     1381 gcgcggcgat ccggcgagcc gtgcgttcag cgtgtgttac ctgcgcgacg gcgagctgat
     1441 cgccgtcgag gcgatcaacc acaccaagga tcagatggcg gcgcgcaagc tgatcccggc
     1501 gcgggcacgg cctgacctgg ccaggctcag tgacccgcaa atcgcgctca aggagaccgt
     1561 ctgacgctgc tttcctgccg gccggtcgca aggtcggcag tcgcgggttc cgacgtctcc
     1621 aggtcgacca tgccgctcaa caccgtgccg gtgcgctgcc tgaccgccgc gagcagtttg
     1681 tcgaatgcat cgctgaagag ctgcacgccc tgctcaagca ggaggtctgt gacttccctg
     1741 atcgcgatgc cctccttctc gagctgcaag agggtttcgg ccgcgccctc caggtcctcg
     1801 acgagactgg cgcgcgcaac gccatgatcg cggaacgcgg caagcgtggc cgggggcatc
     1861 gtgttcaccg tgtcgggccc gatgagctcc tcgacgtaga gcacatcgcg ataggccgga
     1921 tccttggtgc cggtgctggc ccacaacacg cgttgggtct gggcgccgcg ttgcgcgaga
     1981 gaatcccagc gtggaccgcc aaacaattct cgataccgct gatacgtgag cctggcattg
     2041 gcgatcgcca ccttgccgag cagggcgcgc gacaggtcgc gctccttgat gcgcgcatcc
     2101 acgaccgaat cgatgcggct gatgaagaaa ctcgcgacgc tggcaatccc ttcgaggctg
     2161 gcgccattcg cggcgcgatc ctcgagaccg gcgagatacg cctcgacaac ctgcgcgtag
     2221 gcctcctgtg aaaacagcag cgtgacgttg acgttcactg cttcgctgat cagctgacgg
     2281 atggcgacga ttccttcggg tgtggcgggc accttgatca tgaggttcgc ccgcgccacg
     2341 gctttccaga gccggcgtcc ttcttcgatg gttccactcg cgtcgcgcgc cagcttcgga
     2401 gacacctcca ggctcacgta accatcccgc ttcgcggtcc gttcgtaaac gggcaagagg
     2461 acgtcggccg cgtcctggat gtcgcgcacg gcgatcgact cgtacaccgt cttggcgtcg
     2521 gcgttgcgcg cattcgcggc agcgagcacg tcgttgtaat cggcgctgcc ggcgatggcc
     2581 ttttcgaaga tggcggggtt cgaagtcaca cctcgcacac cgtcgtcgat caatcgttgc
     2641 agctcgccac tggtgatgag gtcgcggcgg atgtaatcca gccacaccga ctggccgtga
     2701 agttgcagtg cttcgagtgg atttcccatg ttgctcacct caattgaccc gacagttctt
     2761 gcgtacctgc tcattctgag ggacgcgcca ccggggcgtc agcgcagtca cacgttgtcg
     2821 ccttccgtgg aggtcccgtg gccttttacc acgcgctgcc cccaccgcca tcccgtgatt
     2881 tccggcatgt catcgccgtg ctccgtgatg tactgcctgt gatccagcaa cttctcgttc
     2941 atggcctgct tggaatacgc ggcgcgcgag ccgagttgcg gcagccggtc gatcacatcg
     3001 ccgaagaggt ggaaccggtc gagatcgttc atcaccgcca tgtcgaatgg tcggaagttg
     3061 accagtctcc cctgcagcgc gaaacaggga cctaggtccg attgcgatat ccgggggtcg
     3121 cgccggagca tacgtccgtc atccgtcgcg aagcagttag gcctcttttc gccagcgcgc
     3181 gaatacgcgc tcgatgtcgc cgttcgaatc ctcgcccgcg atgccgctca tgagctcgcg
     3241 acccagctcg cggtaactgt cgaactgcat ctcgccataa aactggttgg acgtgggctg
     3301 gttcggaaaa tccggattgg tgcgccagta gccgtagata tcggcgccga gattcgtgcg
     3361 caacaaagcc ttcaggtaga ggatttcgcc gtggctgccg tcggcgtagc tcacgcgtcc
     3421 gagcaggtgg gcctgtgtac gcagccccgc accgtcgtgg atcaaggggt ccgcgcagat
     3481 gtcgacgctt gcgcccagat ccgcgcgcgc cctctgcacc gcctgtccaa gatcggcaag
     3541 cgtggtgtcg ggatccgcgc cggcatcgct gacgatgatg tagcggcagc gccggcgcag
     3601 cagttcgtac aggccgaggt tttcgaagtg accgccatcg gccaggtgga tacgcgcctg
     3661 tttctcgtcg agcccgacgc cgaacatctc gcgcatcatg agctgatacc acgccggcca
     3721 gcgcaggcgc cgcggttcgt gtgagggatt cgccagccag acgccgagcc ggatgttcag
     3781 cagggccatg agaaagctga cgggccgcga gtgggtggcg tacgtatcgg gatccaccgc
     3841 agcgcccgac accgaaaacg ccgtcgacag cgtcagctcg ccgcggaggt agttagtggt
     3901 gcggcgatat cccgtggcgc tcgacccgca gtacaacggc gtcaggatca tgttctcgcc
     3961 gttgcgcgcg cgcagcttcg cgttggacga actcgtggtg ttcaacgtgg tgttgatgat
     4021 gtggaacggg ccgccggagg tggcgcgcac atccgcaaga cggaacaaca tcggatggcg
     4081 atccgcgccc ccgtgtctgg ggtccactcc cgcgccaacc tcgggcagga acgcttccgc
     4141 gagccggctg cggtagtagc tgtgcatgga gatcgcgttg atgtcgcaca cgaacgcgaa
     4201 caccacggac gccgccagcc acgccatgaa cgccggtgtc tcgagcagca tcgtgtggtg
     4261 ggcgagatgg tagagcgcgg tgaacaggcc gcagaggacc agcgccagtc ccgccaccgc
     4321 aaaggtgccg gaatgcgccc cctgcttgtt ggcctggcgc accgacaaca tgccggtgac
     4381 gaggggaacg atccaggtga gatggcgcgt cagctcctgc gcgaaatcca cgcgaaggta
     4441 ggtggccacg gtctcttcga cgccggcgag caccggcagc aggccgattc cgatgcaggt
     4501 gaagccgcca cccagcaatt gccccagccg ctgccgcagg tattgccccg cgctgagctg
     4561 acggagcggc ggcaacacgg tgctcagcgc gaacgcgagc gtcgccagta gatagagcgc
     4621 gagcagcgcg ataccggcga ttgcgatcag catgaagccg tcatgcgcgg gcaccgagcc
     4681 cgccccgggc atc
//
LOCUS       DATDEU010000068         5127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_741409, whole genome shotgun sequence.
ACCESSION   DATDEU010000068 DATDEU010000000
VERSION     DATDEU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741409"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /gene="hemF"
                     /locus_tag="VIV63_01545"
     CDS             complement(<1..840)
                     /gene="hemF"
                     /locus_tag="VIV63_01545"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY6123306.1"
                     /translation="MSAARPLHVTPIGPEEIQSARDYFLGLHSRLTDTWKSLDAASEQ
                     RRDEWQRPEGDVLTGSGRLSLIENGATFDRAGVAFSDVSGARLPAAASERHTELAGQP
                     FRGMGTSVVVHPVNPYAPTSHANVRLFTARGGEVWWFGGGFDLTPVYPFDEDARAWHE
                     AARAACMPAGPRAYALLKQACDQYFYLPHRGETRGIGGLFADDLNDTTAEIGGNFEHC
                     FALIRRIGDGYLDAYAKLVKLRANTPFGAREQDFQRLRRGRYVEFNLAFDRGTRFGLQ
                     SQAR"
     gene            complement(837..1532)
                     /locus_tag="VIV63_01550"
     CDS             complement(837..1532)
                     /locus_tag="VIV63_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6123307.1"
                     /translation="MVERIAIVEDERLVRELVAVNLRHAGYEIVTAENFADGRQLLAG
                     GEFQLAILDVMLPGGDGFALTRYARDLGINAPILMLTARSDTTSKVRGLDAGADDYLP
                     KPFDVNELLARVRALLRRASGRPATAPPRLAFANFWVRFDTGQAFTNEGEVSLSDKEL
                     RLMEVFSANENRALTRTDLLEEVWGMDQFPTDRTVDNFILRLRKLFEPDPENPVHIVT
                     LRGRGYLFRSAPQ"
     gene            complement(1525..2556)
                     /locus_tag="VIV63_01555"
     CDS             complement(1525..2556)
                     /locus_tag="VIV63_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6123308.1"
                     /translation="MRSPFRQWLAERAELIALGVTLVATASLVFWWTILLRGELNTNF
                     ALERELLAREQGLSAQTLAEREIEVTNRHERRQVMLVGESTLLALMLSGSLVVLFVLA
                     QRRKQQREDMEKLLQFTSHEFKTPVAGVKGLLQSLAIGSIPEAQRAELIKLGQAECDR
                     LEHLAETILAYQRAMTREPREGAQRIDAAQFIGDLVAHRARTSTSGTVEVAAIDAVDV
                     MGDRDGLRVILENLLDNAHKYGGGNVRIAAAHSDGHWRLDISDQGRGFDPANAEKLFD
                     LHNRGSGEGVTHGAGLGLAIARQLARRMGGDVAARSAGPGKGAVFTVTLPLAPQSAAP
                     AAAEGVRHG"
     gene            complement(2556..3893)
                     /locus_tag="VIV63_01560"
     CDS             complement(2556..3893)
                     /locus_tag="VIV63_01560"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY6123309.1"
                     /translation="MTNSSGTPSTAASSSPAKALPLTRSAGFYARAESLIPAGVSSPV
                     RAFRKVGGTPVFFKQAAGAHVTDEDGNRYLDLCMAWGPLILGHAHPRVVEAVQRAAAD
                     GLAFGTAHRSEGELAERVLAAYPYAKLVRFVVSGTEAVATAVRIARASNGRRRILKFD
                     GCYHGHVDSLMVKAGSGVITQGLADSAGVPAQVAADTLVIPLGDVDALEQAFSTQGKD
                     IAAAIIEPLPANNGLLQQSREYLQKLRELTRAHGVVLIFDEVITGFRFGYHGYDKVLG
                     IEPDLTTLGKIVGGGLPVAAVLGRHELLAQLAPLGPVYQAGTMAGNPVCLAAGIATLT
                     ELAEGSPYRHIEMLGRHLDAAYARHALKGQAQLPRVGPIVWPYFDAKAALPVAASSIT
                     STAINEYHRRYRGWLARGVYLPPSAYEVCFLSAAHTTGDVDKLLDVLATSDPN"
     gene            complement(3796..4761)
                     /gene="hemC"
                     /locus_tag="VIV63_01565"
     CDS             complement(3796..4761)
                     /gene="hemC"
                     /locus_tag="VIV63_01565"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY6123310.1"
                     /translation="MTPRNIGSRGSDLALWQSRTVLASLRAVAPAPAWETEITVIATR
                     GDLDQSPLLVGKMEKGFFTRELEIALHERRIDLVVHSLKDLPTAEPEGLWNRTILPRA
                     TPADWLLVRREFYVERDDGMLPLAAGARVGASSLRRGALLGQFAPQARSVPLRGNVPT
                     RLRKLAEGQTVDAIVLAAAGLTRLKLDLNPFAVVELPPEWWIPAPGQGALAAQCRAGD
                     QEIETHVTLLTDAECAAATRWERDFLRVIEGGCSTPFGCYVASGRAHLGIATERGWAA
                     HSIDLPRDIEGPQRDQFIRDAVDGCQLLSRESAATHSLSRVLRAR"
     gene            complement(4758..>5127)
                     /locus_tag="VIV63_01570"
     CDS             complement(4758..>5127)
                     /locus_tag="VIV63_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123311.1"
                     /translation="PERADSPRVFDLGSPLQLLSAPGWRLTALDELLDGNGAVLPEAE
                     YAALEMECAGGAERICQTLLAPPPSDTLAAMTAMRTEFMQNKLPALLEGMPPQRVRKL
                     TSEVNAVLHQFISAARRDAP"
BASE COUNT          857 a         1692 c         1713 g          865 t
ORIGIN      
        1 gcgcgcctgc gactgcaggc caaagcgggt gccacgatcg aacgcgaggt tgaactcgac
       61 gtagcgccct cggcgcaggc gctggaaatc ctgttcccgg gcgccgaatg gcgtgttcgc
      121 gcgcaacttc acgagtttcg cgtaggcgtc gagataaccg tcgccgatgc ggcgaatgag
      181 tgcgaagcag tgttcgaaat tcccgccgat ctccgcggtc gtgtcgttga gatcgtcggc
      241 aaacaacccg ccgatgccgc gggtttcgcc acgatgcggt agatagaagt actggtcgca
      301 ggcctgcttg agcagcgcat aggcgcgtgg tcccgcgggc atgcaggcgg cgcgtgccgc
      361 ttcgtgccag gcgcgcgcgt cttcatcgaa cggatacacc ggcgtcaggt cgaaaccgcc
      421 gccgaaccac cagacttcgc cgccgcgcgc ggtgaacagc cgcacgttgg cgtgcgacgt
      481 cggcgcgtag gggttgacgg gatgcaccac gacgctcgtg cccatgccac gaaacggctg
      541 tcccgcgagc tcggtatggc gctcgctggc agccgcggga agtcgtgcac cgctgacgtc
      601 gctgaacgcg acgccggcgc gatcgaaggt ggccccattt tcgatgagcg acagtcgtcc
      661 gctgcccgtc agtacgtcac cttcgggacg ctgccattca tcgcgacgct gttcgctcgc
      721 cgcatccagc gacttccacg tgtcggtgag gcgcgaatgc aaaccgagga aatagtcgcg
      781 tgccgactgg atttcttcag gcccgatcgg cgtcacatga agtggccggg ctgcgctcac
      841 tgcggcgcac tccggaaaag atagccgcga ccgcgcaacg tgacgatgtg cacggggttc
      901 tcgggatctg gctcgaacaa cttgcgcagg cgcaggatga agttatccac ggtgcggtcg
      961 gtagggaact gatccatgcc ccagacctct tcgagcaggt cggtgcgggt cagcgcgcgg
     1021 ttctcgttgg cggagaacac ttccatgagc cgcagttcct tgtccgacag cgacacctcg
     1081 ccttcgttgg tgaatgcctg gccggtgtcg aagcgtaccc agaagttggc gaatgccagc
     1141 cgcggcggcg cggtggccgg gcggccgctc gcgcggcgca gcagcgcgcg cacgcgcgcc
     1201 agcaattcgt tgacgtcgaa tggcttcggc agatagtcgt cggcgccggc gtcgaggcca
     1261 cgcaccttgg aagtggtgtc gctgcgcgcg gtgagcatga ggatgggcgc attgatgccg
     1321 aggtctcgcg cgtagcgtgt cagcgcgaat ccgtcgccac cgggcagcat cacatcgagg
     1381 atcgcgagct ggaattcgcc gccggccagc aattggcggc catcggcaaa attctccgcg
     1441 gtcacgatct cgtagcccgc gtggcgcaga ttgacggcca ccagctctcg caccagccgc
     1501 tcgtcttcga caatcgctat gcgctcaacc atggcgaacg ccctccgccg ccgcgggcgc
     1561 tgcggattgc ggcgccagtg gcaacgtcac ggtgaaaacc gcgcctttgc ccgggcccgc
     1621 gctgcgcgca gccacgtcac cgcccatgcg gcgcgcgagt tgccgcgcaa tagcaaggcc
     1681 cagtcccgcg ccgtgagtga cgccttcgcc gctgccacgg ttgtgcaggt cgaacaactt
     1741 ttccgcgttc gccggatcga aaccgcggcc ctgatcgctg atgtcgagac gccagtgtcc
     1801 gtcgctgtgg gcagccgcga tgcgcacgtt gccgccgcca tacttgtgtg cgttgtcgag
     1861 caggttctcg aggatcacgc gcaatccgtc gcggtctccc atgacatcga ccgcgtcgat
     1921 ggccgcgact tccaccgtac cgctggtcga ggtgcgtgca cggtgcgcca cgaggtcacc
     1981 gatgaattgc gccgcgtcga tgcgctgcgc gccttcgcgg ggttcgcgcg tcatcgcacg
     2041 ctggtaggcg agtatggttt ccgccagatg ctcgagccga tcgcactcgg cctgtcccag
     2101 cttgatgagc tcagcacgct gggcttccgg aatgctgccg atcgccaggc tctgcaaaag
     2161 ccctttcaca ccggccaccg gagtcttgaa ctcgtggctg gtgaattgca gcagtttctc
     2221 catgtcttcg cgctgctgct tgcggcgctg cgcgagcacg aacagcacca ccaggctgcc
     2281 ggagagcatc aacgcgagca gcgtggattc accgaccagc atgacctggc ggcgttcgtg
     2341 acgattcgtg acttcgattt cgcgttccgc caacgtctgc gccgacagcc cctgctcgcg
     2401 cgccagcaat tcgcgttcca gcgcgaagtt ggtgttcaac tcgccgcgca acaggatggt
     2461 ccaccagaac accagtgacg cggtcgccac cagcgtcacg ccgagcgcga tgagctcggc
     2521 gcgttccgca agccattgtc gaaacgggga tcgcatcagt tcggatcgct cgttgccagc
     2581 acgtccagca gtttatccac atcgccggtg gtatgagccg cggacaggaa acacacctca
     2641 taagccgagg gcggcagata gacaccgcgc gcgagccagc cgcgatagcg ccggtgatat
     2701 tcgttgatcg ccgtcgacgt gatcgacgac gcggcgacgg gtagcgccgc cttcgcgtcg
     2761 aagtacggcc agacgatggg cccgacccgg ggaagctgcg cttgtccctt gagcgcatgg
     2821 cgcgcgtatg ccgcatccag gtgcctgcct agcatctcga tgtgccggta ggggctgcct
     2881 tcggcgagct cagtcagcgt cgcgatgccg gccgccaggc acaccggatt tccggccatg
     2941 gtgcccgcct gatacaccgg gcccagcggc gccagttgcg ccagcaattc gtgccggccg
     3001 agcaccgccg cgacgggcag gccaccgccg acgatcttgc cgagcgtggt gagatcgggc
     3061 tcgatgccga gcaccttgtc gtatccgtga tagccgaaac gaaaacccgt gatcacctca
     3121 tcgaaaatga ggacgacgcc gtgcgcgcgc gtgagctcgc gcagcttctg cagatattcg
     3181 cgcgactgtt gcagcagccc gttgttggcc gggagtggct cgatgatcgc cgcggcgatg
     3241 tccttgccct gggtgctgaa cgcctgctcg agtgcatcga cgtcgccgag cggaatgacc
     3301 agcgtgtcgg cagcgacctg cgcgggcacg cccgcgctgt cggcgagacc ctgcgtgatg
     3361 acaccgctgc cggccttcac catcagcgag tccacgtgtc cgtgataaca accgtcgaac
     3421 ttgagaatgc gccggcggcc gttggacgcg cgcgcgatgc gcacggcggt ggcaacggct
     3481 tcggtgccgc tgacgacgaa ccgcaccagc ttggcgtagg gataggccgc cagcactcgc
     3541 tcggcgagct caccttcact gcgatgcgcg gtgccaaaag ccagtccatc cgccgcggcg
     3601 cgctgcacgg cctcgaccac gcgcggatgt gcgtgcccga ggatcagcgg cccccaggcc
     3661 atgcagagat cgagatagcg attgccgtct tcgtcggtga cgtgggcgcc cgcggcctgc
     3721 ttgaagaaca caggcgtgcc gccgaccttg cggaacgcgc gaaccggact cgacacaccc
     3781 gccgggatca gcgactcagc gcgcgcgtag aacccggctg agcgagtgag tggcagcgct
     3841 ttcgcgggag aggagctggc agccgtcgac ggcgtcccgg atgaattggt cacgttgagg
     3901 tccttcaatg tctcgtggca ggtcgatact gtgcgcggcc cagccgcgct cggtggcaat
     3961 tccgagatgc gcgcggccgc tcgcgacgta gcaaccgaag ggcgtcgaac agcctccttc
     4021 gatgacgcgc aggaaatcgc gttcccagcg agtggccgcg gcgcactccg cgtcggtgag
     4081 cagggtgacg tgggtttcga tttcctgatc gccggcgcgg cattgcgccg ccagggctcc
     4141 ctgccccggc gcgggaatcc accactcggg tggcagctcg acgactgcga acggattcag
     4201 gtcgagcttc agtcgggtga ggccggccgc ggccagcacg atggcatcga cagtctgacc
     4261 ctcggcgagt ttgcgaagac gcgtcggcac gttgccgcgc aacggcaccg atctcgcctg
     4321 cggcgcgaac tggcctaaca aggcgccgcg acgcagcgac gacgcgccga ctcgcgcacc
     4381 ggccgccaac ggcaacattc cgtcgtcacg ctccacatag aattcacggc gcaccagcag
     4441 ccagtcggcc ggcgtggcgc gcggcagtat cgtgcgattc cagagtcctt ccggttcggc
     4501 cgtgggcaga tccttgaggg aatgcaccac gaggtcgatg cgccgctcgt ggagtgcgat
     4561 ctcgagttcg cgcgtgaaga agcccttctc catttttccg accagcagcg gcgactggtc
     4621 gaggtcaccg cgtgtggcga tgacggtgat ttccgtctcc caggcgggcg cgggcgccac
     4681 ggcgcgcagg gacgcgagca ccgtgcgtga ttgccacaac gcgaggtcgc tgccgcggct
     4741 gccgatgttc ctgggcgtca tggcgcgtcg cgcctcgccg cggagatgaa ctggtgcagg
     4801 accgcgttga cctccgaagt cagcttgcgc acacgttgag gcggcatgcc ttcgagcaag
     4861 gcgggtagtt tgttttgcat gaattcggta cgcatggcgg tcatcgcggc gagtgtgtcg
     4921 ctcggtggag gcgccagcaa tgtctggcag atcctttccg cgccacccgc gcactccatt
     4981 tcgagcgccg cgtattcggc ttccgggagg accgcgccgt tcccatccag caattcatcg
     5041 agcgccgtga gccgccagcc gggcgcggac aggagttgca gcggactgcc gagatcgaac
     5101 acacgcggcg aatctgcacg ctccggc
//
LOCUS       DATDEU010000069         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_303585, whole genome shotgun sequence.
ACCESSION   DATDEU010000069 DATDEU010000000
VERSION     DATDEU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303585"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1008
                     /locus_tag="VIV63_01575"
     CDS             172..1008
                     /locus_tag="VIV63_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY6123312.1"
                     /translation="MGGPCEVLIDVDDEVVARKVTDAVAACAWRIENKFSRYRKDNIV
                     ARINASAGEPVQVDEETANLLDYAELLHRLSDGLFDITSGVLRRAWTFDGGNRTPARF
                     ELDALLPLVGWSKLCWKRPVITLRPGMQIDFGGIGKEYAVDLAAKAAAELSNAAVLVN
                     FGGDLAVTRVRDGGKPWRVGIEGIGSGIQRAARLVDLTAGALATSGDTYRFIESNGQR
                     LSHILDPRDGCPVRDAPRSVTVAAPTCTQAGMLTTLAMLRGPGAEAFLEAEGVRHWIQ
                     RA"
     gene            1147..1890
                     /locus_tag="VIV63_01580"
     CDS             1147..1890
                     /locus_tag="VIV63_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY6123313.1"
                     /translation="MRQLRTYPVWDTSVRWFHWINVLCVLGLVAVGLAILNDKALGVS
                     NDGKILLKTVHVWIGYVFALNLFWRLVWGFIGGIHSRWRAILPLGRAYPAALRRYVAA
                     LRAGEHPQYLGHNPLGRIAVTLLLLLLVTMAATGLVLAGTDLFLAPFGSTIARWVAAP
                     GVDPATLVPYAPEMYDKSSYAAMRALRAPFVRVHEFAFYGLLIMGVIHIAAVVLTEVR
                     GGGNLVSAMITGKKILRAPLSDQNKDASP"
BASE COUNT          354 a          655 c          658 g          372 t
ORIGIN      
        1 cgtgcgactg gagtactacc agcagaaggc aaaggcgcca ggcgaagttc tgatcggcaa
       61 ccaggtcggg aattcgcaga tgccggactt caacgcggtg atcctgcagt tcggctacca
      121 cttcaagctc tgatccgcgg cgaaggcctg tgggcaggcc gcttccaggc catgggcggg
      181 ccgtgtgaag tgctcatcga cgttgacgat gaagtcgtgg cgcgcaaagt aaccgacgcc
      241 gtagctgcct gcgcctggcg aatcgagaac aagttcagtc gatatcgcaa ggacaacatc
      301 gtggcgcgca tcaacgcatc tgcgggcgaa ccggtgcagg tcgacgagga gactgcaaac
      361 ctcctggact acgcggagct gttgcaccgg ctttccgatg ggctgttcga catcacatcg
      421 ggcgtgcttc gccgcgcgtg gaccttcgac ggcggcaacc gcacgcccgc gcgcttcgag
      481 ctcgatgcgc tgctgccgct cgtaggctgg tcgaagttgt gctggaagcg acccgtgatc
      541 acgctgcggc cgggcatgca gatcgatttc ggcggcattg gcaaggagta cgccgtcgac
      601 ctggcggcga aagccgctgc ggaactctcg aatgccgccg tgctcgtcaa cttcggcggc
      661 gatctcgccg tcacgcgcgt acgcgacggc ggcaagccgt ggcgcgtggg catcgaaggc
      721 atcgggtccg gaatccaacg cgccgcgcgg ctcgtggatc tcaccgccgg ggcgctggcc
      781 acgagcggcg acacttaccg gttcatcgaa tcgaacgggc agcgcttgtc ccatatcctg
      841 gatccgcgcg atggctgccc ggtacgcgac gcgcctcgct ctgtcaccgt ggccgcgccc
      901 acctgcacac aggctggcat gctgaccacg ctggccatgc taagaggccc cggggccgaa
      961 gcctttctcg aggccgaggg cgtaaggcac tggatacaac gcgcttgaca ctgcacgccg
     1021 cgccgccaga cctgatctag ttagggcgca ttcccgcctc gcggcgaagc ggctccatac
     1081 gtgggactac gaacgccaaa ccgtacggat tcgcgcatag tgccttcccg aaaccgagtc
     1141 cgatccatgc gccaattgag aacctacccc gtgtgggaca ccagcgtgcg ctggttccac
     1201 tggatcaacg tgctgtgcgt gctcggcctg gtcgccgtgg gcctggcgat cctcaacgac
     1261 aaggcgctgg gcgtcagcaa cgacggcaag atcctgctga agaccgtgca cgtctggatc
     1321 ggttacgtgt tcgcgctgaa tcttttctgg cggctggtgt ggggattcat cggcggcatt
     1381 cattcgcgtt ggcgcgccat cctgcctctg ggtcgcgcgt atcccgcggc attgcgcagg
     1441 tacgtcgccg cgctgcgcgc cggcgaacat cctcagtacc tcggacacaa tccgctcggc
     1501 cgcatcgccg tgacgctgtt gttgctgctg ctcgtgacga tggcggcaac cggcctcgtg
     1561 cttgcgggca ccgacctctt tcttgcgccc ttcggctcca cgatcgcccg atgggtcgcg
     1621 gcgcccggtg tcgacccggc aacgctggtg ccgtacgcgc cggagatgta cgacaagagt
     1681 tcctacgccg ccatgcgcgc gctgcgcgcg ccgttcgttc gcgttcacga gttcgccttc
     1741 tatggcctgc tcatcatggg cgtcatccac atcgccgcgg tggtgctgac cgaggtgcgt
     1801 ggcggcggaa atctcgtgtc ggcgatgatc acgggaaaga agatcctgcg ggcaccgctg
     1861 tcggaccaga acaaggacgc aagcccctga ttcggcaaat gtaacggcac gcatacagta
     1921 ctgcgcgcag gctggtactg gttatgcatg aggcggggtc gccgcttaaa cttcgtggtt
     1981 tccattcact tgcacgagaa accgcgcttg accatccagc agataaagat tcgcggcgc
//
LOCUS       DATDEU010000070         9025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_450153, whole genome shotgun sequence.
ACCESSION   DATDEU010000070 DATDEU010000000
VERSION     DATDEU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450153"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..359
                     /locus_tag="VIV63_01585"
     CDS             156..359
                     /locus_tag="VIV63_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123314.1"
                     /translation="MTIPATRDSDSIDQTYAAGQFGAQPGERWRRFLERVGTRSNDFA
                     VRLSRARFVYMHRRDIATHWLPL"
     gene            complement(523..915)
                     /locus_tag="VIV63_01590"
     CDS             complement(523..915)
                     /locus_tag="VIV63_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY6123315.1"
                     /translation="MSMQSSSAEPQLNATPLIDVMLVLLVILIITLPAATHAVKLDMP
                     REGGAAPPRTVYLDVAYGGHVYWNGAYVESMTDLSSRLVELAAQDVGARVEVVPENLA
                     PYEKVAQVLAAAQRAHISRLGVRSVPDR"
     gene            979..1266
                     /locus_tag="VIV63_01595"
     CDS             979..1266
                     /locus_tag="VIV63_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123316.1"
                     /translation="MRAEPRFKPLPLEAATLLSEGKVHEALKVLRRTEGIGRGDARRR
                     VDAYIAQEPLLRVQIETQLGARRRRLFFWFLLVDLAITAGIIYWFFYRGSA"
     gene            1266..1904
                     /locus_tag="VIV63_01600"
     CDS             1266..1904
                     /locus_tag="VIV63_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6123317.1"
                     /translation="MLLIGMFDSPFVRRVAISMKLLGVPFEHGNWSVGKDFDRIRHYN
                     PLGRVPTLVTDQGETLMESGAILDYLDELAGPERALLPRAGTERRQALNLMAMATGAA
                     EKGLLQVYERVFRPEPKRHQPWVDRCRLQMSSSLAAIDRHLGERGLAQWLVGKRMTQA
                     DITAACVFTFLNDSLRVAEDAVMFQSLATLASRCESMPVFQETRVPFHAPSS"
     gene            1962..4328
                     /locus_tag="VIV63_01605"
     CDS             1962..4328
                     /locus_tag="VIV63_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123318.1"
                     /translation="MKKSAMSLMALAAACSGVQAGVGEWTSAGIPGAYVHRLEYVTGG
                     VAIAAALNGIYRTTNHGSTWTLVREVPLPYLTGIAANRTNPDQVLVAADNLLRSSNGG
                     ATFQTVTVSGGASPTGPMAVGFSRDGAFAWVIEDEGHVWRSADGGATWTRLNGMLPPN
                     NYFWIEPDAADRNTLYAGPGMGILISRDAGDTWNPLAVAGAMYAVTSSRSQPGSALAI
                     NTGPYNITRTIDYGANWTPVNAGTESIFRLATGPGGSAIAGDYLANVYTSQDDGASFA
                     LRGRLPNAEPIWLAFNPTDSLHILAATQAGVVGSSDGGATWQERIQGLLEPRVFNVAV
                     ARDGSNAVYAAATDLASIYRRDATTGVFTGVGRSSTPVLGYPGFQQYGIPDYRIAVSP
                     QDSRTVYMIRDGHFGRSINGGETWTRLSVTGRMNTLALDPVNPLVMYAGGLLANLKSV
                     DGGFNWTPLAGGLPQSVAQFHVDPTNNASVYALVNAFGSAQAPLYRSADGGMTWTPAA
                     WTGAASWQLASMAFEPGRISTIYLAGGSGLFRSTDSGASWTELMPVTSVPLAGMFDVA
                     VDPQSPSVVYASAYSGLVRSADGGATWGDVFSTTAAGAFHQMFDIDLMPGHNARLIGL
                     RQGGGIYEADVAVRLELSRTPTTITAGQPGTIAVTVRNNSSLAATRVRVTSSLPAASG
                     SYAVQATGAACAVAVSELTCDIGTLPAAASAVMSIYFTPAAAGTVTFAMSSYEVLTPG
                     ATPATTVTVASAGPSGSSGGGGGGGGRIDYLLLAMLAALQIRGALRRR"
     gene            complement(4315..5472)
                     /locus_tag="VIV63_01610"
     CDS             complement(4315..5472)
                     /locus_tag="VIV63_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008002122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6123319.1"
                     /translation="MLNPLDLYDIRGLLTEDERMVQDSVGRFVDEKVLPVIQDCFENH
                     TFPKELVPELADLGLLGSSIQGYDCAGLNAVSYGLICQELERGDSGIRSFVSVQSSLC
                     MYPIYAFGSEEQKQKWLPDMARGRKIGCFGLTEPHGGSDPSNMKTHAKKKGGDWVING
                     SKMWITNGAIADLCICWAMTEDGIRGFIVEKGMKGFAAPEIERKFSLRASVTSALFFD
                     NVVVPEANLLPGVKGLKGPLSCLTQARYGITWGVIGAAQACLAQLLDYTKTREMFGKP
                     LAGTQAIQLRLAEMARKITGAQLMSHQLGRLKDLGTMQPAQVSLAKWNNCRIALDIAR
                     DCRDMLGGSGISAEFVPIRHALNLESVITYEGTETIHQLTIGRELTGINAF"
     gene            complement(5487..5954)
                     /locus_tag="VIV63_01615"
     CDS             complement(5487..5954)
                     /locus_tag="VIV63_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEY6123320.1"
                     /translation="MRTVEQWFGEYGESHSNPRNELLHIICVPPIVLTVIGFIWSIPV
                     PAAFAEISPWLNWATITIVLAVIYYFTLSATLGLGAAIALGILAFIVRWLDTLAWPLW
                     LTCLVIFVIGWIGQFIGHSIEGKRPSFMKDIQFLLIGPLWLLGHLYRRLGISY"
     gene            6011..6535
                     /locus_tag="VIV63_01620"
     CDS             6011..6535
                     /locus_tag="VIV63_01620"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEY6123321.1"
                     /translation="MPLMQRIMKLVAGSGIFPDARLLELYPPFIPMRIKVLEIADQWR
                     TVRILLPLNSRSRNPGGVMFGGYQAALADPIAALACARVFPGYSVWTRSMTISFDHGG
                     NSDLEMRFTLTPEQEKDIRMRLESDGRATPTFEYGYVRSDGVRCTVIRNTVAIRPRGY
                     KRATSPPASSEAVD"
     gene            6616..7293
                     /locus_tag="VIV63_01625"
     CDS             6616..7293
                     /locus_tag="VIV63_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6123322.1"
                     /translation="MNKMFLRTTLLAMAAAITVAGCQTTDAYTGEKKVNNTTKGAGIG
                     AAAGAVLGALTGDNSKERRERALIGAGVGALTGAGVGAYMDKQEAKLRAQLQGTGVSV
                     TRNGNDLILNMPGNITFKTASSDLNAGFYKVLDSVTLVLKEFDKTLIDVEGHTDNVGD
                     DSYNQNLSVQRATSVGSYLQSHGVNSQRIVTVGAGESRPVASNDNADGRQQNRRVELK
                     LQPITAG"
     gene            complement(7367..8215)
                     /locus_tag="VIV63_01630"
     CDS             complement(7367..8215)
                     /locus_tag="VIV63_01630"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6123323.1"
                     /translation="MTKSALNPQGEQMADESMIRTLAAQIEAIWPQEKSLINFYRLPR
                     NARVLDVGAGTGEFSARVAELLPEATVTGVELLAASVEFARERHATLSPRLSFETGDA
                     FHLTQPDDTFDLVANRHMIQSVPHVDRILAELVRVTKPGGRVHVLAEDYSMLHMMSGP
                     LDPDVFWRKGPVEFSMRTNTDSRIGRRAWTLLREAGLVDVRVDYVVVDTLRVPRRVFA
                     DIITAWRDGYVNALSTPDFPVAVVRQHFNEIIASVLNPDHYAVWHVPIVSGVKPKSTT
                     VPGIVS"
     gene            complement(8329..>9025)
                     /gene="rpoH"
                     /locus_tag="VIV63_01635"
     CDS             complement(8329..>9025)
                     /gene="rpoH"
                     /locus_tag="VIV63_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY6123324.1"
                     /translation="LSHLRFVVHIARGYSGYGLPLGDVVQEGNVGLMKAVKRFDPTVG
                     VRLVSFAVHWIRAEIHEYVLRNWRLVKVATTKAQRKLFFNLRKMKKNLAWLSHDETLA
                     VARDLKVTPAEVTEMEKRLAARDLSFDPVPDSGSEDGDETYSPAAYLPAPDSDPAVQI
                     ENAEWDDTTGDRLAQAMKTLEPRARDILVSRWTGEGKATLHDLAGKYGVSAERVRQIE
                     ANAIKKLRGLMTA"
BASE COUNT         1609 a         2845 c         2923 g         1648 t
ORIGIN      
        1 gtttcccggg agcgcgcagt gtaagcgacc ggaacaggcg cgggtggagt ggccacattt
       61 ggtcactttc ggtcggcgcg gcgccatcaa cgcgtcacgg gatggccggt ggacgggcgc
      121 gaaatggtcc tcacgatcca gaccgaggac acgccatgac gattccagcc acccgtgaca
      181 gcgactccat cgatcagaca tatgccgccg gacagttcgg cgcgcagccg ggggaacggt
      241 ggcgccggtt cctcgagcgc gtcggcacgc gcagcaacga cttcgccgtg cggttatcgc
      301 gcgcgcgctt cgtctacatg catcggcgcg acatcgccac gcactggttg ccgctatgag
      361 cgccaaaccc gctgaagcgc ccgttcacga cgaagggggt ggctcgcaca tccagtggct
      421 ggccatcgcc gtgttgttca tctgctggtt cgccgaagcc gtcatgatct gaaagacgcc
      481 ggcgcgaatt ggcgccggct atctacgctc gaccggcgac gctcaacggt ccggtacgct
      541 gcggacaccc aggcggctga tgtgcgcacg ctgcgcggcc gccagcacct gcgccacttt
      601 ttcatagggt gcgaggttct ccggcaccac ttcgacgcgc gccccgacat cctgggcggc
      661 gagttcgacg aggcgcgacg acagatccgt catcgactcg acgtacgcgc cattccagta
      721 cacgtggcca ccgtaggcga cgtccagata gacagtgcgg ggcggcgcgg caccgccttc
      781 gcgcggcatg tcgagtttga cagcgtgggt cgccgccggc agggtaatga tcaggatcac
      841 cagcagcacg agcatcacgt cgatcaatgg cgtggcgttg agctgcggct cggcacttga
      901 ggattgcatc gacatgagcc acctcctgta tggcctaacg cgcgccggcg ccgcgcaggg
      961 ttttgccgcg ggtagccgat gagggcggag cccaggttca aaccactgcc tctcgaggcc
     1021 gccacgctgc tcagcgaagg caaggtccat gaagccctca aggtcctgcg gcgcaccgaa
     1081 ggcattggcc gcggcgacgc gaggcggcgc gtcgacgcgt acattgcgca ggagccgctg
     1141 ttgcgcgtgc aaatcgagac gcagctgggc gcgaggcggc gaaggctctt cttctggttc
     1201 ctgctcgtgg acctcgcgat caccgcaggg atcatctact ggttcttcta ccgaggttca
     1261 gcctgatgtt gttgatcgga atgttcgatt cgccgttcgt gcgccgcgtg gccatttcca
     1321 tgaagttgtt aggcgtgccg ttcgagcacg gcaactggtc ggtgggcaag gatttcgatc
     1381 gcatccgcca ctacaacccg ctcggccgcg tgccgacgct cgtgaccgac cagggcgaaa
     1441 cgctcatgga atccggcgcg atcctcgatt acctcgacga actcgcgggt cccgagcgcg
     1501 cgttgctgcc gcgtgccggc accgaacgcc gtcaggccct gaatctcatg gccatggcca
     1561 cgggtgcggc ggaaaaaggc cttctgcagg tatacgaacg tgtcttcagg cccgagccga
     1621 agcggcacca gccgtgggtg gatcgctgcc gcctgcagat gagctcgtcg ctcgccgcca
     1681 tcgatcgtca cctgggtgag cgtggacttg cgcaatggct ggtgggcaaa cgcatgacgc
     1741 aggcggacat cactgccgcg tgtgtattca cgtttctcaa cgactcgctg cgcgtggccg
     1801 aagacgcggt gatgttccag tcgctggcca cgctggcgtc gcgctgcgaa tccatgcccg
     1861 tgttccagga aacgcgcgtg ccattccatg cgccgagcag ttagcggctg gaagcacgca
     1921 ttgtcaggat tcggcccgcc atgacagatt tgtcgaacca tatgaagaag tcagcgatga
     1981 gcctgatggc gctggccgcc gcgtgttccg gtgtgcaagc cggcgtgggc gaatggacgt
     2041 ccgcgggtat tcccggggcc tacgtccatc gattggaata tgtcaccggt ggcgtcgcga
     2101 tcgccgccgc cctcaacggc atctaccgca cgaccaatca tggttcgaca tggaccttgg
     2161 tgcgcgaagt cccgttgcca tacctgaccg gcattgccgc gaaccggacg aatccggacc
     2221 aggtgctggt cgccgcggac aatctcctgc gcagctcaaa tggtggcgcg actttccaga
     2281 ccgtcaccgt gagtggcggc gcgagcccga cgggtccgat ggctgtcggg ttcagccggg
     2341 acggcgcgtt cgcctgggtc atcgaggacg aaggacacgt ctggcgcagc gccgacggcg
     2401 gcgcgacgtg gacgcggctc aatggcatgt tgccgcccaa caattacttc tggatcgaac
     2461 ccgacgccgc cgaccgcaac acgctgtatg cgggtcccgg catgggcatc ctgatttcgc
     2521 gcgatgccgg ggacacgtgg aatccgctcg ccgtcgcggg cgccatgtat gcggtcacgt
     2581 cttcgcggtc gcagcccggg tctgcgctgg ccatcaacac agggccctac aacatcaccc
     2641 ggacgatcga ctacggcgcg aactggacgc cggtcaacgc cggcacggaa agcatattca
     2701 gactggcaac gggtcccggc ggcagcgcga tcgccggtga ctacctggca aacgtctaca
     2761 cgtcgcagga cgacggcgcg tcgttcgccc tccgcggacg gctgccgaac gcagaaccca
     2821 tctggcttgc gttcaatccg accgactcac ttcatattct cgcggcgacg caggccggag
     2881 tcgtcggttc gagcgacggt ggcgcgacct ggcaggaacg catccagggt ctgctcgagc
     2941 cgcgcgtgtt caatgtcgca gtggcgcgcg acggctcgaa tgccgtctat gccgccgcga
     3001 ccgatctcgc ctctatctac cggcgcgatg cgaccacggg tgtgttcacg ggcgtgggac
     3061 gctcgtcgac gcccgtgctg ggctatccgg ggttccagca atacgggatt ccggactacc
     3121 gcatcgccgt ctcgccgcag gattcgcgga ccgtctacat gattcgcgac gggcattttg
     3181 gccggtcgat caacggcggc gagacctgga cgcggctatc cgtcacgggg cgcatgaaca
     3241 ccctggctct cgatccggtg aatccactgg tgatgtacgc aggtggcctg ctggcgaacc
     3301 tcaagtcggt cgacggcgga ttcaactgga caccgttggc gggcggcttg cctcaaagcg
     3361 tcgcccagtt ccatgtcgat ccgaccaaca acgccagtgt gtacgcgctg gtgaatgcgt
     3421 tcggcagcgc gcaagcgccg ctttacagga gcgccgacgg cgggatgacc tggacgccgg
     3481 ccgcctggac cggcgccgcg tcctggcagc tggcttcgat ggccttcgag ccgggacgga
     3541 tatcgactat ctacctcgcg ggcgggagcg gcctgttccg gtcgacggat tctggtgcat
     3601 cctggacgga gctcatgcca gtgacttcag ttccgcttgc gggcatgttc gatgtcgccg
     3661 tcgatccgca atcgccgtcc gtggtgtatg catccgccta ttcaggtctc gtgcgatccg
     3721 cggatggtgg cgccacctgg ggagacgtgt tctcgaccac cgcggcgggt gcgtttcatc
     3781 agatgtttga catcgatctg atgccggggc acaacgcgcg cctcatcggc cttcggcaag
     3841 gcggcggcat ctacgaagcg gatgtcgccg tacgcctcga actgagccgc acgcccacca
     3901 ccatcacggc gggacagccc ggaacgatcg cggtcaccgt gcgcaacaat tcgtcgctgg
     3961 cggccacgcg tgtccgcgtg acgtcgtcat tgccggcggc ctcgggaagt tacgccgtgc
     4021 aggcaaccgg tgcggcctgc gcggtggcgg tcagtgaatt gacctgtgac atcggcacgc
     4081 tgccggcagc ggcatcagcg gtgatgtcga tctacttcac gccggccgcc gcgggtacgg
     4141 tcacgttcgc gatgtcgtct tatgaagtgc tgactcccgg cgcgacgccg gcgaccacgg
     4201 tcaccgtggc cagcgcgggt ccttcaggct catcgggcgg cgggggagga ggcggaggcc
     4261 gcatcgacta tctactgctc gcgatgctcg ccgctctgca gatccgcgga gcgctcagaa
     4321 ggcgttgatg cccgtcagct cgcggccgat cgtgagctgg tggatggttt ccgtgccttc
     4381 ataggtgatc acgctctcga gattcagcgc gtgccggatc ggcacgaatt cggcgctgat
     4441 tcccgatcca ccgagcatgt cgcggcaatc tcgcgcgatg tcgagcgcga tgcggcaatt
     4501 gttccacttc gccagcgaca cctgcgcggg ctgcatcgtg cccaggtcct tgagcctgcc
     4561 gagctggtgg ctcatcaact gggcgccggt gatcttgcgc gccatttccg cgagcctcag
     4621 ctggatggcc tgtgtgcccg ccagtggctt gccgaacatc tcgcgcgtct tcgtgtagtc
     4681 gagcaactgc gcgagacagg cctgcgccgc gccgatgacc ccccaggtga tgccgtatcg
     4741 cgcctgtgtg aggcacgaca gcggaccttt gaggcctttc acgcctggaa gcaggttggc
     4801 ttccggcacg acgacgttgt cgaagaacag cgccgacgtc accgaggcgc gcagcgaaaa
     4861 cttgcgttcg atttccggcg cggcgaaccc tttcatgcct ttctcgacga tgaatccgcg
     4921 gatgccgtct tccgtcatcg cccagcagat gcagaggtcg gcgatggcgc cgttggtgat
     4981 ccacatcttc gagccattga tgacccagtc gccgcctttc ttcttcgcgt gggtcttcat
     5041 gttcgaagga tccgacccgc cgtgcggttc ggtgaggccg aagcagccga tcttccggcc
     5101 gcgcgccatg tcgggcagcc acttctgttt ctgctcttca ctgccgaacg cgtagatcgg
     5161 atacatgcac aacgagctct gcacggagac gaagctgcgg attccggaat cgccgcgctc
     5221 cagctcctgg cagatgagcc cgtagctgac ggcgttcagg cccgcgcagt cgtaaccctg
     5281 gatggacgac cccagcaatc cgagatcggc gagctcgggc accagttctt tcgggaacgt
     5341 gtggttctcg aagcaatcct ggatgacggg cagcactttc tcgtcgacga aacggccgac
     5401 ggagtcctgc accatgcgtt catcctcggt cagcaggccg cggatgtcgt agagatcgag
     5461 gggattcagc atcgcgcgat gatacgtcag tacgaaatgc cgaggcgccg gtacagatgg
     5521 ccgagcaacc acagagggcc gatcagcagg aactggatgt ccttcatgaa ggacggccgc
     5581 ttgccttcga tggagtggcc gatgaactgg ccgatccagc cgatgacgaa gatgaccagg
     5641 caggtgagcc acagcggcca ggcgagggtg tcgagccacc gcacgatgaa cgcgagtatg
     5701 ccgagcgcga tggcggcgcc cagtcccagc gtcgccgaca gcgtgaagta gtagatgacg
     5761 gcgagcacga tggtgatcgt cgcccagttg agccagggcg aaatttccgc gaaggccgcg
     5821 ggcaccggta tcgaccagat gaagccgatg accgtgagca cgatgggcgg cacgcagatg
     5881 atgtgcagca attcattgcg gggattgctg tggctttcgc catattcccc gaaccattgc
     5941 tccacggtgc gcatggcgcg gaacgctacg ctgcatcaca ctgcttcgca agaatgaaga
     6001 ggacgcccgc atgccgctga tgcagcgaat catgaagttg gttgccggca gcggcatttt
     6061 tcccgacgca cgcctgttag agctgtaccc gccgttcatc cccatgcgca tcaaggtgct
     6121 ggagatcgcc gatcaatggc gcaccgtccg gatcctgctg ccgctgaatt cccgctcgcg
     6181 aaatcccgga ggcgtgatgt ttggcggtta ccaggcggcg ctcgccgatc cgatcgcggc
     6241 gctggcgtgt gcgcgggttt tcccgggtta ctcggtctgg acacgttcca tgactatctc
     6301 tttcgatcac ggcggaaact ccgacctcga gatgaggttt acgcttacgc cggaacagga
     6361 aaaggatatt aggatgcggc tcgaaagtga cggtcgcgcc acaccgacat ttgaatatgg
     6421 ctacgttcgc agcgatggtg tgcgctgcac ggtcattcgc aatacggttg cgattcggcc
     6481 gcgcggctac aaacgcgcca cctcgccgcc ggcttcctcc gaagctgtcg actgagaact
     6541 ggctcacaga aaaacggttc attcggcgtt cagatgcacg ccgctatgtt tgcccgcaga
     6601 ttccaggaga ctttcatgaa caagatgttt ttacgcacga cattgttagc catggccgcc
     6661 gccatcacgg ttgccggctg ccagaccacc gacgcctata cgggcgagaa gaaggtcaac
     6721 aacaccacca agggcgctgg catcggcgcg gcagcgggtg cagtgctcgg cgctctgacg
     6781 ggcgacaatt ccaaggaacg ccgcgaacgt gcgctgatcg gcgcgggtgt cggtgctctc
     6841 accggcgcag gcgtcggcgc atacatggac aagcaggaag ccaagctgcg cgcgcagctt
     6901 cagggcaccg gagtgtccgt gacgcgcaat ggcaatgacc tcatcctcaa catgcccgga
     6961 aacatcacgt tcaagaccgc gagttcagat ctgaacgcgg gcttctacaa ggtgctcgat
     7021 tcggtgacgc tcgttctcaa ggagttcgac aagaccctca tcgacgtcga aggccacacg
     7081 gacaacgtgg gtgacgactc ctataaccag aacctgtcgg ttcagcgcgc gacttcggtg
     7141 ggcagctacc tgcagtcgca tggtgtgaac agccagcgca tcgtcacggt gggtgccggt
     7201 gagtcccgcc cggtggcgtc gaacgacaac gccgatggcc gccagcagaa ccgccgcgtg
     7261 gagctgaagc ttcagccgat caccgcgggc tgatcttcag agtcaaagat tttgacggac
     7321 gccggccttt taggccggcg ttttcctatg tggcgcttgc gcctgactaa gaaacgatgc
     7381 ctggcaccgt agtcgatttt ggtttcacac ctgaaacaat aggcacgtgc cagaccgcat
     7441 agtgatccgg attcagcacg ctcgcgatga tttcattgaa gtgctggcgc accaccgcga
     7501 cggggaaatc cggcgtcgac agcgcattca catagccatc gcgccaggct gtgatgatgt
     7561 cggcgaagac gcggcgcggc acccgcagcg tgtccaccac tacgtagtcg acacgcacgt
     7621 cgacgagccc cgcttcgcgc agcagcgtcc acgcgcggcg gccgatgcgc gaatccgtgt
     7681 tggtgcgcat cgaaaattcc acgggtccct tgcgccagaa gacatccggg tcgagcggcc
     7741 cggacatcat gtggagcatc gagtagtcct cggccagcac gtgcacgcgc ccacccggtt
     7801 tggtgacccg gaccagctcg gcgaggatgc ggtccacatg gggaaccgac tggatcatgt
     7861 ggcggttcgc caccaggtcg aaagtgtcgt ccggctgggt gaggtgaaac gcatcgccgg
     7921 tctcgaacga caggcgcgga ctcagggttg cgtgacgctc gcgcgcgaac tcgacggacg
     7981 ccgccagcag ttcgaccccg gtcacggtgg cttcgggcaa tagttcggcc acgcgcgcgc
     8041 tgaactcacc ggtgcccgcg cccacgtcga gcacccgcgc gttacgcggc aaacggtaga
     8101 agttgatgag cgacttttcc tgcggccaga tggcctcgat ctgcgccgcc agcgtgcgaa
     8161 tcatggattc gtccgccatc tgttcgccct ggggattgag tgctgatttc gtcatggcaa
     8221 cggattaatc catggactcg tgggtcgcaa caacacaaaa acttagcatc ggaaaagaaa
     8281 agccccggtt tacggccggg gcttcaagct tgagtctgaa cgggcgcttc aggccgtcat
     8341 caggcctcgc agtttcttga tggcgttcgc ctcgatctgg cgtacgcgtt ccgccgacac
     8401 gccgtatttc ccggcgaggt cgtgcagcgt ggctttgcct tcgccagtcc agcggctgac
     8461 caggatgtcg cgcgcgcgcg gctccagggt cttcatggcc tgggccaggc gatcgcccgt
     8521 ggtgtcgtcc cactcggcat tctcgatctg aacagcagga tcagaatcgg gcgccggtag
     8581 ataggcggcg ggcgagtagg tctcgtcgcc gtcttcgctg ccggaatccg gaaccgggtc
     8641 gaacgagagg tcgcgcgccg cgaggcgctt ttccatctcg gtgacttccg cgggcgtgac
     8701 cttgaggtcg cgggccacgg ccagcgtttc gtcgtgcgac agccacgcca ggttcttttt
     8761 catcttgcgc agattgaaga acagcttgcg ctgcgccttg gtggtggcga ccttcacgag
     8821 gcgccagttg cgcagtacgt attcgtggat ttcggcacgg atccagtgga ccgcgaacga
     8881 caccaggcgg acgccgaccg tggggtcgaa gcgcttcacg gccttcatga ggccgacgtt
     8941 gccttcctgc acgacgtcgc cgagcggcag gccgtagccc gagtaaccgc gcgcgatgtg
     9001 cacgacgaaa cggaggtgcg acaac
//
LOCUS       DATDEU010000071         2796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1471215, whole genome shotgun sequence.
ACCESSION   DATDEU010000071 DATDEU010000000
VERSION     DATDEU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471215"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VIV63_01640"
     CDS             complement(<1..852)
                     /locus_tag="VIV63_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123325.1"
                     /translation="MRSFHGFFLSCGLALLTASPSRADSFETKITIRADQPGAVINPN
                     IYGQFVEHLGRGVYEGIWVGPDSRIPNTRGIRNDVVAALRKIRIPLVRWPGGCFAETY
                     HWRDAIGPRAQRPRGVNTAWGDEVETNDFGTHEFMDFVEQVGAQAFISVNVASGSPAE
                     AQAWMQYLTGPASSGAGQERARNGRAEPWKIPFIGIGNETWGCGGNMTAEYYSDLYRQ
                     YVSVLKPYGRLIASDANSDDYGWTETLLKRALHRHSADIPTKLAYISKQPQIDMISVH
                     FYTFSGND"
     gene            1151..>2796
                     /locus_tag="VIV63_01645"
     CDS             1151..>2796
                     /locus_tag="VIV63_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6123326.1"
                     /translation="MTSRTIFRAFTRASKFTMALAAATASAQTPDPNATSATSEEMST
                     ITVTGSRTITDNAQSPTPLTTVDLAELMQTTPSDTADALNKLPTILGGRTPRTQGNGS
                     TNNGGNTLSLRNFGPSRTLVLLDGHRIAPNNQDGTVNIDALPSMLMQRVDIVTGGASA
                     IYGSDAVAGVVNYVLDKEFTGLTVKADYGLSDYGDGDQYQVGAAWGTDLFSDRGHFEV
                     AARMRHQDMIPMSERPYAEDGQAWLLVGGGTQGNPFRNINYGRVISQAQYGNINCGTA
                     CPFNGYTFNQAGVPSPLVHGTPVPGTNLESGGDGGYVKYGTFRSEIEMKDVFARFDVD
                     IGESANWYVQGSWAQAENASDWINWVASTANGRPSAIFANNPFLDANAQTALGANVVC
                     PPIPTGFAWRCLPAVPPASTVNGTTPPAPPTTPYLSVPSYIWNNVDGQPANGSPNRMY
                     RTLGDQQSWNVETGATGEIGSWKWDAYYNHGNSELTVTNPNNTDNAKYLASLDAVLSG
                     GTIQCWVTTQPAFAALYPGCVPTNLVDPAGPSAAAYDYLRT"
BASE COUNT          548 a          902 c          827 g          519 t
ORIGIN      
        1 gtcgtttccc gagaatgtgt agaagtgcac cgagatcatg tcgatctgcg gttgcttgct
       61 gatgtatgcc agcttggtcg gtatgtcggc agaatggcgg tggagcgcgc gcttcaagag
      121 cgtttccgtc cacccgtagt cgtccgaatt ggcgtcggaa gcgatcagtc ttccatacgg
      181 cttgagaacg ctgacgtact ggcgataaag gtccgagtaa tactcggcgg tcatgttgcc
      241 gccgcacccc caggtctcgt tgccgatacc gatgaacgga atcttccagg gttccgcgcg
      301 gccattgcgc gcgcgctcct gaccggcacc cgagctggcc ggccccgtga ggtattgcat
      361 ccaggcctga gcttccgcag gcgatccgct tgcaacattg accgaaatga aagcctgggc
      421 gcccacttgt tcgacgaagt ccatgaactc gtgggttccg aagtcgttgg tctcgacttc
      481 gtcgccccac gcggtgttca cgccacgcgg tcgttgcgcg cgtgggccaa tcgcgtcccg
      541 ccagtggtag gtttcagcga aacatccgcc tggccatcgc accagcggaa tccggatttt
      601 ccgcagcgcc gcgacgacgt cgttgcggat cccgcgtgtg ttggggattc tggaatccgg
      661 tcccacccag attccttcgt acacgccgcg gccaaggtgt tcgacgaact gtccatagat
      721 gttgggattg atcacggcgc ctggctggtc tgcgcgaatg gtgatcttcg tttcgaacga
      781 atcggcccgg gatggcgaag cggtcagcaa cgccaggccg caagaaagga aaaatccgtg
      841 gaagcttctc atgtgtcctc tttttttcgc gccggcagcg ccggatcctg ccgtcgcgtt
      901 gcaagtgtaa ccacgcacga cccgagcgtt gaaaccgggc gggacgccgt cgttgctaac
      961 tatcgcggta agtactgacc accggtgtca ctcgcatttc acgggccgcg aggcgccaat
     1021 agcaaaatat gcatagagac atacgattcc tgtaactaca ttgccttagt aaaaataggc
     1081 tgctagcgtc tcgacaggat cacgatcagg ggcgctttcc gttgcctcgt cgttgatcgg
     1141 gggggatggc atgacttcac ggacaatttt ccgggcgttc acgcgggcgt cgaaattcac
     1201 catggctctc gcggccgcta cggcctcggc gcagacgccc gatccgaacg ctaccagcgc
     1261 taccagcgag gaaatgagca ccatcacggt gaccggctcc cgcacgatca ccgataacgc
     1321 tcaatcgccg acgccgctca ccacggtgga cctcgccgag ctcatgcaga cgactccgag
     1381 cgatactgcc gacgcgctga acaagctgcc caccatcctg ggcggccgca cgccgcgcac
     1441 ccagggcaac ggcagcacga acaacggcgg caacaccttg tcgcttcgca acttcggacc
     1501 ttcgcgcacg ctggtgctgc tggacggcca ccgcattgcg cctaacaacc aggacggcac
     1561 ggtcaacatc gacgcgctgc cgtcgatgct gatgcaacgc gtcgacatcg tgaccggtgg
     1621 cgcatcggcc atctacggct cggacgccgt cgccggcgtg gtcaactacg tgctggacaa
     1681 ggaattcacc ggcctgaccg tgaaagcgga ctacggctta tcggactacg gcgatggcga
     1741 ccagtatcag gtcggcgcgg cctggggcac ggacctcttt tccgatcgcg gccatttcga
     1801 agtggccgca cgcatgcgcc atcaggacat gatcccgatg agcgagcgtc cgtacgccga
     1861 ggacggacag gcctggttgc tcgtaggcgg tggcacgcag ggcaacccgt tcaggaacat
     1921 caactatggg cgcgtgatca gccaagccca gtacggcaac atcaattgcg gcaccgcatg
     1981 ccccttcaac ggctatacgt tcaaccaggc tggcgtgccg agccccctgg ttcacggcac
     2041 accggtcccc ggaaccaacc tcgaatcggg tggagacggt ggctacgtga agtacggcac
     2101 cttccgttca gaaatcgaga tgaaggatgt gttcgcgcgc ttcgacgtgg acatcggcga
     2161 aagcgccaac tggtacgtgc agggatcctg ggcccaggct gaaaacgcgt ctgactggat
     2221 caactgggtg gcaagcacag cgaacggccg ccccagcgcg atcttcgcca acaatccatt
     2281 ccttgacgcg aacgcgcaga ctgccttggg tgcgaatgtc gtttgccccc caattcccac
     2341 tggttttgcc tggcgctgtc tgccggccgt gccgcccgcc tcgacggtta atggcaccac
     2401 gcctccggcg cccccgacca cgccctacct ctccgttccg tcgtacatct ggaacaacgt
     2461 cgatgggcag cccgccaatg gcagccccaa ccgcatgtat cgcacgctcg gcgatcagca
     2521 gtcgtggaac gtggaaaccg gagccaccgg tgagatcggt agttggaaat gggatgcgta
     2581 ctacaaccac ggcaacagcg agctgacagt caccaatccg aacaacacgg acaacgccaa
     2641 atatctcgct tcgctcgatg cggtgctctc tggcggcact atccagtgct gggtgaccac
     2701 acagccggcg ttcgccgccc tctacccggg ctgcgttccg accaatctcg tcgatcccgc
     2761 cggaccttcc gcggcggcct atgactacct gcgaac
//
LOCUS       DATDEU010000072         3813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_12470, whole genome shotgun sequence.
ACCESSION   DATDEU010000072 DATDEU010000000
VERSION     DATDEU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12470"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VIV63_01650"
     CDS             <1..342
                     /locus_tag="VIV63_01650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY6123327.1"
                     /translation="AMNGGALCAEPSVPLVRIAELEIDPAQLDKYKAAVKEEMEISVR
                     VEPGVLAIYSVALKEDESKLRFFEIYADEAAYRAHIASPHFKRYVEITKDMIRSRKLI
                     ETVPVQLSAKK"
     gene            466..1035
                     /locus_tag="VIV63_01655"
     CDS             466..1035
                     /locus_tag="VIV63_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123328.1"
                     /translation="MNNKGLAVSLLLTGVFAVTAAVPGQTGEQGPGATQQKQHSGELV
                     RVVRENTRRFRDVNQAEVEGYHLMFGCVSGPDDGAMGLHYVNMELVVDGALDPRRPEI
                     VIYEPLPNGGRKLIGADFLVFAEAWHANNTATPELGGQLMHLIESPNRYGLPAFYTLH
                     VWAWKPNPTGAFVNWHSDVSCEQYDGAQE"
     gene            1038..1838
                     /locus_tag="VIV63_01660"
     CDS             1038..1838
                     /locus_tag="VIV63_01660"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY6123329.1"
                     /translation="MSAALYRPTAFVFSIACYGVFLLVFLYLLAFLGNLQATPLADAF
                     SWIPTLVPRSIDLGHDMGSLGLAVAVDTGLILLFGVQHSVMARPGFKRVWTRTVPKEM
                     ERSVYVLIASAVLALLMWQWRPIPTPVFWHADGAWSAALGWSVMGAGVAVLLWATFLI
                     NHFELFGLQQGWITLRGGEIRGPAFVTPWLYKIVRHPLYVGWLMIFWGVPTMTAGHFL
                     FSAGMSGYILIAIRYEERDLVESIGEPYERYRQQVPALVPQPGRRFVS"
     gene            complement(1831..3675)
                     /locus_tag="VIV63_01665"
     CDS             complement(1831..3675)
                     /locus_tag="VIV63_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY6123330.1"
                     /translation="MAFTRQDLQAGFRIGECLIEPRQNRIVRRDTELRLEPRVMDVLI
                     CLAENAGEVVSREKLNAQVWGNLVVTDQAVTNCISELRNHLGDDRAAHRVIETISKRG
                     YRLLAPVELARVEVPAAADKPPPVARRPLRVGVAILAVALLAGLGWWWWREASTPTVS
                     SVAVLRFENAAGDASLDYLSLALSDEMATLLTKSRDLAVRPFNYVTGDDPLAAARMRG
                     VDHIVSGRYYLEESGQLSLAIEAQQVLQERVIWRTRITVPAGDLLAMRGRIAEGVSEG
                     LLPALGARTVTTAGSMPAHDETYRLYLRSLALAQQPKPTERAIEMLERAVTLEPAFAS
                     AWQALGLRYYDYGTWFTGSEPMRQKSLAAHRKALELDPDLISAARNIVTHRTEAGDLE
                     GAYLDARRLLEHFGPGADTHFSIAYVYRYGGMMEASQRHCELALDRDPRDPRLRSCGY
                     AYLHAGKLSRVMEFFKLDEGTFFTNWATVLYLMRLNDSSAALQIATQAADEPTRRLMQ
                     PCLEGVRGAALDVPVTEFMEHWQKTGDPEAFYAIAPMLSYCGRNPGALRFLERALEGG
                     ICPYPALDLDPIWSGLRGDPEFQRIRAEAMVCHDRFRKFVAANGGPSN"
BASE COUNT          685 a         1221 c         1237 g          670 t
ORIGIN      
        1 gccatgaatg gcggcgccct ctgcgcggaa ccttccgttc ctctcgtgcg catcgccgaa
       61 ctggaaatcg acccggcgca gctcgacaaa tacaaagccg ccgtcaagga agagatggaa
      121 atctcggtcc gcgtggagcc gggtgtgctg gccatctatt cggtggcgct gaaagaagac
      181 gagtcgaaac ttcgcttctt cgaaatctac gcggacgaag ccgcgtatcg cgcgcacatc
      241 gcatcaccgc acttcaagag atacgtggag atcacgaaag acatgatccg gtctcgcaag
      301 ctgatagaga cagtccccgt ccagctcagc gcaaagaagt agcagctccc ggcgcccgcc
      361 tttgcaaatc cggagctttt ccgtatgtcc tcgtgaggac tccggaggca ctccggcggt
      421 ttagtcgtcc ctcgtgaatc agccaatcac atgagggata cgatcatgaa caacaaaggc
      481 ttagcggtct ccttgctact taccggcgtc ttcgcggtca cagcggccgt tcccggccag
      541 accggggagc aaggccccgg cgccacgcag caaaaacaac attcgggcga actggtccgg
      601 gtcgtccgcg aaaacacgcg gcgattccgc gacgtgaacc aggccgaggt cgagggctat
      661 cacctgatgt tcggctgcgt gagcgggccg gacgacggcg cgatgggctt gcactacgtg
      721 aacatggagc tggtggtgga cggggcgctg gacccgcgcc ggcccgaaat cgtcatctac
      781 gaaccgttgc ccaatggcgg ccgcaagctg atcggcgcgg acttcctggt gttcgccgaa
      841 gcctggcatg cgaacaacac ggcaacgccg gaactcggcg ggcagctcat gcacctgatc
      901 gagagtccga atcgttatgg cctgccggcg ttctacacgc tgcacgtgtg ggcgtggaaa
      961 ccgaacccga ccggcgcatt cgtgaactgg cactccgacg tgtcctgcga acaatacgac
     1021 ggcgcgcagg agtgagcatg tcagccgcgc tctaccgccc caccgcgttc gtcttcagca
     1081 tcgcctgcta cggcgtgttc ctgctggtgt tcctgtacct gctcgccttt ctgggcaacc
     1141 tgcaggcaac accgctggcc gatgccttct catggatccc gacgctggtg ccgagatcca
     1201 tcgatctcgg acacgacatg gggtcgttag gcctcgcggt cgcagtggac accggactga
     1261 tactgctctt cggtgtccag cactcggtga tggcgcggcc cgggttcaag cgcgtgtgga
     1321 ctcggaccgt gcccaaggaa atggagcgaa gcgtctatgt gctgatcgcg agcgccgtgc
     1381 tcgcgctgct catgtggcag tggcggccga tcccgacgcc cgtgttctgg cacgccgacg
     1441 gcgcatggag cgcggcgctc ggctggtcag tcatgggcgc aggggtcgcc gttctgctct
     1501 gggcgacgtt cctgatcaac catttcgaac tgttcggact ccagcagggc tggatcacgc
     1561 tgcgcggcgg cgagattcgc ggtcccgcat tcgtcacgcc ctggctatac aagatcgtgc
     1621 gccacccgct gtacgtcggg tggctcatga tcttctgggg cgtgcccacc atgaccgccg
     1681 gacacttcct gttctcggcg ggcatgagcg gatacatcct gatcgcgatc cgctacgagg
     1741 agcgcgatct cgtggagagc atcggcgagc cgtacgagcg ctaccggcag caggtgccgg
     1801 cgctggttcc ccagcccgga cggcgcttcg ttagttagac gggccgccgt tggcggcgac
     1861 gaacttccga aaccggtcgt ggcagaccat ggcctcggcg cggattcgct ggaattccgg
     1921 atcgccgcgc aggcccgacc agatcggatc gaggtcgagc gccggataag gacagatgcc
     1981 gccctccagg gcgcgctcca ggaaacggag cgcccccggg ttgcggccgc aataggacaa
     2041 catgggcgcg atcgcataga acgcttcagg gtcgcccgtc ttctgccaat gctccatgaa
     2101 ctccgtgacg ggcacatcga gcgccgcgcc gcggacgcct tcgagacagg gctgcatcag
     2161 tcgccgcgtg ggctcgtcag cggcctgcgt ggcgatctgc agcgcggcac tgctgtcatt
     2221 gagccgcatc aggtacaaca cggtggccca gttggtgaag aatgttcctt catcgagttt
     2281 gaagaattcc atgacacgcg agagctttcc tgcatgtaga taggcatagc cgcaggaccg
     2341 caggcgcggg tcacgcgggt cgcgatcgag cgcgagctcg cagtgacgtt gtgacgcctc
     2401 catcatgccg ccgtaccggt agacgtacgc gatgctgaag tgcgtgtcgg cacccgggcc
     2461 gaagtgttcg agcagtcgac gggcatccag gtaggctccc tcgagatctc cggcctcggt
     2521 tcgatgcgtc acgatattgc gcgccgccga gatgagatcc ggatcgagtt ccagcgcctt
     2581 gcgatgcgcg gccagggact tctgtcgcat cggctcggag ccggtgaacc acgtgccgta
     2641 gtcgtagtaa cgaaggccaa gcgcctgcca tgccgacgcg aacgccggct cgagcgtcac
     2701 ggcgcgctcc agcatctcga tggcgcgctc ggtgggtttg ggctgttgcg ccagggcgag
     2761 actgcgtaga tagagccgat acgtctcgtc gtgggcgggc atcgatccgg cggtcgtgac
     2821 ggtgcgcgcg ccgagggccg gcagcaaccc ttcactcacg ccctccgcaa tccgcccacg
     2881 catcgccagc agatcgcccg cgggcaccgt gatgcgcgtg cgccagatca cgcgctcctg
     2941 aaggacttgc tgagcctcga tggcgagact caattgaccg ctctcctcga ggtagtagcg
     3001 gccgctcacg atgtgatcca cgccgcgcat gcgcgccgcg gcgagcggat cgtcacccgt
     3061 cacatagttg aacggacgta cggccaggtc gcgcgacttg gtgagcagcg tcgccatttc
     3121 atcggagagc gccagcgata gatagtccag cgatgcatcg ccggccgcgt tctcgaagcg
     3181 cagcaccgcg acgctgctga ccgtgggcgt cgacgcctcc cgccaccacc accacccgag
     3241 gcccgccagc aacgcgaccg ccagtatcgc gacacccacg cgcaacggcc gacgcgcaac
     3301 cggcggtggt ttgtcagcgg ccgcaggcac ttcgacgcga gccagttcca caggcgcgag
     3361 cagccgatag ccgcgcttcg aaatggtttc gatgacgcgg tgggcggcgc gatcgtcgcc
     3421 caggtggttg cgcagttcgg agatgcagtt ggtcactgcc tggtcggtga cgacgaggtt
     3481 gccccacacc tgcgcgttca gcttttcgcg cgagacgact tcgcctgcgt tttcggcgag
     3541 gcaaatcagc acgtccatga ctcgaggctc gagccggagc tcggtgtcgc gccgcacgat
     3601 gcgattctga cgaggctcga tcaggcactc gccaattcgg aagcccgcct gcaggtcctg
     3661 acgcgtgaat gccatgttgg gggcgatgct acctcaggtg agtgcatcca ccggcgtggg
     3721 tcgtgtagtg ggagtgcctg atacgcctgg cgctatcgaa gcccgctgat caatcgatga
     3781 aagcgcggct cgccacgcag attggaatag tcc
//
LOCUS       DATDEU010000073         4252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1325629, whole genome shotgun sequence.
ACCESSION   DATDEU010000073 DATDEU010000000
VERSION     DATDEU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325629"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1908
                     /locus_tag="VIV63_01670"
     CDS             <1..1908
                     /locus_tag="VIV63_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123331.1"
                     /translation="VKTGGNTASNKGESWTRELSTSFEWQPIERLMIKGAAQFVRSEV
                     DSKGYDVFPEMNLPGTYSLDLTGDFPEVAFTSPSSAEDPANTRWFAHMPNRQFNKGEM
                     DAFNLDAEFSISEDGFFRSVRAGARYADRRENDNGYYGWTNLCAGWDGCDQATRTFDQ
                     AQEGDVQFQTFPEFFRGEANLPSGIWMPSFARVGQLDPDAIDAEYGINSADINVQAHN
                     NFTFIPTDSREQQIVNEAAYAMVRFAAFEDGALPIDGNLGVRVVRIENTSNGFYDQRG
                     MFATDQDGNRINIANIPNLYDGSLTRTGGTTVTKGLPSINLRFKASDSVQVRLAYGVT
                     MDQPQFNDLRATGSIGANRTNDVFNGTYNADTGNPLLKPAISKNSDLSLEWYRGGATA
                     HLALFHKEIKNALAYTSGVKDVELFLNDGTTTTIQANRNEVQNSTALSMIKGFEIGGR
                     VFFDRLPAPWNGFGVEANYTYVDSESPGEVYFDIDGGEHRDAPVRGLSEDTYNLQLMF
                     EKPKFGARLAWSWRSEYLLSTNSNGTNGDYWYFPTPNLGAANTIRPTFADIALPTYSD
                     QYGQLDFGASYRPTENLSVSLDFRNLLDEISKTFTTGYPNAETGEYDTKVARSWFISD
                     RRVTLGVRYKF"
     gene            1982..3529
                     /locus_tag="VIV63_01675"
     CDS             1982..3529
                     /locus_tag="VIV63_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6123332.1"
                     /translation="MVRSVLIVGGGTAGWITAGYLARMLGADSPAGVRICLVESADLG
                     VIGVGEGTFPTIRRTLQRIGISEARLIRECGATFKQGIRFVNWRHDPSAQRSHYMHSF
                     QSTQNASGLDLLSYWLLGVAGQDESWAEVNTPQKIVSDAVLAPKLITHSDFKGPLNYA
                     YHVDAVKLAHLLRKVAIEAGVQHIVDTIDGVNLGEDGAIRSVVSRNRGELSADLYVDC
                     TGFRAQLIGQALEVPDKPCGSVLFCDTALAIQVPYDQPADPVAAYTISTAHEAGWTWD
                     IGLDTRRGIGYVYSSSHTDDTRAEEVLRAYAGKAARNVSARKIQFNSGYKETHWYKNC
                     VAIGLSSGFFEPLEATGIILVEAAVATLAKLFPWGGDFDVSARQFNRQMCRRYERALA
                     FIKAHYCISERRDTPFWRDNIDANSIPDELDDLLARWRHRPPGEIDFDLNTDIFTEHS
                     WQYVLYGMGYKTDLAPRAGLFKFHEEARQAFAEIRRQAQFACRTLPAHRELLQAALTR
                     EFGEARS"
     gene            3526..>4252
                     /locus_tag="VIV63_01680"
     CDS             3526..>4252
                     /locus_tag="VIV63_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6123333.1"
                     /translation="MSDRLIKNIVIVGGGTAGWMCAAALSKVLRGRYRIVLVESDEIG
                     TVGVGEATIPHIRTFNDLLEIDENDFIRATQGTFKLGIEFVDWGKIGDRYIHGFGRVG
                     QNLWTIAFEQYWQRMHRLGKAPELSDYCINHVAARSNRFMRPTREVKNSPLNEIAYAF
                     HFDAGLYARFLRKFSEQRGVERLEGRIVDVALHGETGHITSVKLASGAQVEGELFLDC
                     SGFRGLLIEQALGTGYEEWTHWLP"
BASE COUNT          838 a         1341 c         1330 g          743 t
ORIGIN      
        1 gtgaagaccg gtggcaacac cgcgtctaac aagggcgaaa gctggactcg cgaactgagc
       61 accagcttcg agtggcagcc catcgagcga ttgatgatca agggcgctgc gcagttcgtg
      121 cggtcggaag tcgacagcaa gggctacgac gtcttcccgg aaatgaatct gccaggcact
      181 tacagccttg acctcacggg cgacttcccg gaggtggcgt tcaccagtcc gtcttccgcc
      241 gaggacccgg cgaacacgcg ctggtttgcg cacatgccca atcggcagtt caacaaaggc
      301 gagatggacg cgttcaacct ggatgccgaa ttctccatct ccgaagatgg gttcttccgc
      361 tcggttcgtg cgggtgcgcg ttacgcggat cgccgcgaaa acgacaacgg ctactacggg
      421 tggaccaatc tgtgcgccgg gtgggacggc tgcgaccagg ccacccgtac cttcgaccag
      481 gcgcaggaag gcgacgttca gttccagacc ttcccggagt tcttccgcgg tgaagccaac
      541 ctgccatccg gcatctggat gccgagcttc gcgcgcgtgg ggcaactcga ccctgatgcc
      601 atcgacgccg aatacggaat caacagtgca gacatcaacg tgcaggcgca caacaatttc
      661 acgttcatcc cgaccgacag ccgcgagcag caaatcgtca acgaggcggc ctacgccatg
      721 gtgcgattcg ccgctttcga agacggcgca ctgccgatcg atggcaacct cggcgtgcgc
      781 gtcgttcgca tcgagaacac cagcaacggc ttctacgatc agcgaggcat gttcgccacc
      841 gaccaggacg gcaaccggat caacatcgcg aacatcccca atctgtacga cggctcattg
      901 acccggaccg ggggcacgac ggtcaccaag ggcttgccct cgataaacct gcgcttcaag
      961 gccagcgatt ccgtgcaggt tcggctcgcc tacggcgtga ccatggacca gccgcaattc
     1021 aacgacctga gagccaccgg cagcatcggc gcgaaccgca ccaacgacgt gttcaacggc
     1081 acctacaatg ccgacacggg caacccgctg ctgaagcccg cgatttccaa gaacagcgac
     1141 ctctcgctcg agtggtatcg cggtggcgcg accgcgcatc tcgccctgtt ccacaaggag
     1201 atcaagaacg cgctggccta cacgagcggc gtgaaggatg tcgagttgtt cctgaacgac
     1261 ggcaccacga ccaccatcca ggccaatcgc aacgaggtgc agaactcgac ggcgttgtcg
     1321 atgatcaagg gcttcgagat cggagggcgg gtgttcttcg atcgcctgcc cgcgccgtgg
     1381 aatggcttcg gcgtggaggc caactatacg tacgtcgaca gcgagtcgcc gggagaggtg
     1441 tatttcgaca tcgacggcgg cgagcaccgc gatgccccgg tgcgcggtct ttccgaagac
     1501 acttacaacc tgcagctgat gttcgagaag ccgaagttcg gcgcgcggct ggcgtggagc
     1561 tggcgcagcg agtacctgtt gagcacgaac agcaacggca ccaatggtga ttactggtat
     1621 ttcccgacgc cgaatctcgg cgcggcgaac accatcaggc cgaccttcgc ggatatcgcc
     1681 ctgccgactt actcggacca atacggccag ctggacttcg gtgcctcgta tcgcccgacg
     1741 gaaaatctgt cggtgtcgct cgacttcagg aatctgctgg atgagatcag caagaccttc
     1801 acgacggggt atccgaatgc cgagaccggc gaatacgaca cgaaggtggc gcgcagctgg
     1861 ttcatcagcg accggcgcgt gacgctgggc gtgaggtaca agttctaggc gggtcccgca
     1921 cgacctggcc agatcgggcg ccttgcgcaa gcaagccgcc cattctccgg ggctcagcgt
     1981 gatggtgcga agcgtcctga ttgtcggcgg cggaaccgcg ggttggatca ccgcaggtta
     2041 tctcgcgcgc atgctcggag cggattctcc agccggcgtg cggatctgcc tggtcgagtc
     2101 cgccgacctg ggagtgatcg gagtcggtga gggcaccttt ccaacgattc gccgcacgct
     2161 gcagcgcatc ggcatttccg aagcccgcct catcagggag tgcggcgcca ccttcaagca
     2221 gggaatccgc ttcgtgaact ggcggcatga cccatccgcg cagcgcagcc actacatgca
     2281 ttccttccag agcacccaga acgcctctgg cctcgatctg ctttcctatt ggctgctcgg
     2341 tgtcgctggt caggatgaga gttgggccga ggtgaacacg ccccagaaga tcgtgtcgga
     2401 cgctgtgctt gcgcccaagc tgatcacgca ttcggatttc aaaggcccgc tgaattacgc
     2461 ctatcacgtc gacgcggtga agctggccca tctgcttcgc aaggtcgcca tcgaggccgg
     2521 cgtgcagcac atcgtcgaca cgatcgacgg cgtgaacctg ggcgaggacg gcgccattcg
     2581 ttccgtcgtc tcccggaatc gcggcgagct gagtgcggac ctgtacgtag actgcaccgg
     2641 gttccgtgcg cagctcatag gccaggcgct cgaggtgccc gacaagccct gcggttcggt
     2701 gttgttctgc gataccgcgc tggcgataca ggtgccatac gatcaacccg cggacccggt
     2761 cgcggcgtac accatatcca cggcgcacga ggcgggctgg acctgggaca tcgggctcga
     2821 tacgcggcgc ggcatcggct acgtgtattc ctcgtcccat accgatgaca cccgggccga
     2881 agaggtgctg cgtgcttacg ccggcaaagc ggcccggaac gtcagcgcac gcaagatcca
     2941 gttcaactcc ggatacaagg agacccactg gtacaagaac tgcgtcgcca tcggcctttc
     3001 cagcggcttt ttcgagcccc tggaggcgac gggcatcatc ctggtggagg cggcggtggc
     3061 cacgctcgcg aagttgtttc cctggggcgg tgacttcgac gtgtccgccc gccaattcaa
     3121 ccggcagatg tgccgacggt atgaacgcgc gctggccttc atcaaggcgc attactgcat
     3181 cagcgagcgg cgggatacgc cgttctggcg cgacaacatc gacgccaaca gtattcccga
     3241 cgagctcgac gacctgctcg cccgatggcg acatcgacca cccggcgaaa tcgatttcga
     3301 tttgaacacc gacatcttca ccgagcacag ctggcagtac gtgctctacg ggatgggtta
     3361 caagaccgac ctggcgccgc gagccggact gttcaaattc cacgaggagg cacggcaggc
     3421 gttcgcggag atccggcgcc aggcgcagtt cgcctgccgc acgcttcccg cgcatcgcga
     3481 gctgctgcaa gcggcactga cccgcgagtt cggcgaggcg cgctcatgag cgaccggctg
     3541 atcaagaaca tcgtgatcgt cggtggcggc accgctggct ggatgtgcgc cgccgcgctc
     3601 tcgaaagtgc tgcgaggtcg ctatcgcatc gtgctggtcg aatccgacga aatcggcact
     3661 gtcggggtgg gcgaggccac gattccgcac attcggactt tcaacgacct gctcgagatc
     3721 gacgagaacg acttcatccg tgccacgcag ggaacgttca agctgggcat cgagttcgtc
     3781 gattggggaa aaatcggcga ccgctacatc cacggcttcg gtcgcgtcgg ccagaacctc
     3841 tggaccatcg ccttcgagca gtactggcag cgcatgcacc ggctcggcaa ggcgcccgag
     3901 ctcagcgact actgcatcaa tcatgtcgcc gcgcgcagca acagattcat gcggcccacc
     3961 cgcgaagtga agaactcgcc gctgaacgag atcgcgtatg cgttccactt cgatgcgggg
     4021 ctgtatgccc ggttcctgcg caagttcagc gagcagcgtg gggtcgagcg cctcgaaggc
     4081 aggatcgtgg atgtcgcgct ccatggcgaa acggggcaca tcacctcggt caagctcgcg
     4141 agcggcgcgc aggtggaagg ggagttgttc ctcgactgtt cgggctttcg cggtctcttg
     4201 atcgagcagg cgcttggcac gggctacgaa gagtggacgc attggctgcc at
//
LOCUS       DATDEU010000074         6410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_742545, whole genome shotgun sequence.
ACCESSION   DATDEU010000074 DATDEU010000000
VERSION     DATDEU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742545"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VIV63_01685"
     CDS             <1..465
                     /locus_tag="VIV63_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123334.1"
                     /translation="KDAELISRLRRLDAAAATAEPGFGYEGLIGRHAEKQNRARRRHS
                     FARGTASLLVVAMVGVGVWRFEQEEGVRTVEVIAPEVASDSRAAQPRIVRADTYFAVA
                     ALEDHIASIDDALNDARAARGTAEVARLERTRELLVDSYTRVRYAEMVNANF"
     gene            475..1473
                     /locus_tag="VIV63_01690"
     CDS             475..1473
                     /locus_tag="VIV63_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY6123335.1"
                     /translation="MKKLMLICLLGLSAAATAQQTKSVDAQVSRDEARRDAEEAKTRA
                     KLDEARTRLDKAARDVAELSNQLGANVRHDIRIVEGGPRKAVLGIQVDPDSGSDGVRV
                     LSVSPGGPAAEAGLRDTDVIVSLNGVATIGGDPDRTLITQMRTVKPEQKVKVRVLRDG
                     KNKDFTVVARPMAFDERRFNVRLPEGGAMGVNMPMVQHFRAFFPGEFGGMELASITPR
                     LGAYFGVTEGVLVVQAPENDAFKLEDGDVLQAIDGRKPEDGGHAMRILRSYKSGEKLN
                     LTVLRQRKPVTLAVTMPERPEFGDNMMFGTGPAPLMPPVPPAPPVPGTPGSGAGTF"
     gene            1508..2539
                     /gene="queA"
                     /locus_tag="VIV63_01695"
     CDS             1508..2539
                     /gene="queA"
                     /locus_tag="VIV63_01695"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY6123336.1"
                     /translation="MRRSDFTYDLPEVLIAQQPLAERSASRLLTLDGATGALADRQMR
                     DLPELVSPGDLLIFNDTKVIPARLFAIKESGGKVELLLERPLDSRSALVHARASKPLR
                     PPMTLQGRGGRIEVVEKRGDLWVIELPEAPLAFFERYGQMPLPPYIRREPDAADSTRY
                     QSVFAKRQGAVAAPTASLHFDEPLLAALTGRGAHVAFVTLHVGAGTFQPVRTDAVGAH
                     VMHAEFVEVSDAAVAAVAAARARGGRVIAVGTTVVRALESAAGGGSLSPYIGDSSLFI
                     VPGYRFNVVDAMVTNFHLPESTLLMLVSAFAGREAVLAAYRHAVAGQYRFFSYGDAMF
                     VTPALNAHA"
     gene            2550..3695
                     /gene="tgt"
                     /locus_tag="VIV63_01700"
     CDS             2550..3695
                     /gene="tgt"
                     /locus_tag="VIV63_01700"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY6123337.1"
                     /translation="MTAEFTLLGTAGLARRGRLKLAHGTVETPAFMPVGTYGTVKAMT
                     PEELEGLGAEIVLGNTFHLMLRPGNEVLRAHGGLHGFMNWRRPILTDSGGFQVFSLES
                     LRKITEEGVQFRSPIDGSAVRLTPEDSMDTQLALGSDIAMALDDCTPYPATEQQARDS
                     MERSMRWAARSHAHYYRPDAHGNAPPGELFAIVQGGMFLPLRHASLETLQKLPWKGFA
                     VGGLAVGEPEEDRLRVLEGLLPHMPADKPRYLMGVGRPQDIIAAVARGIDMFDCVMPT
                     RHARNGHLFTSQGVVNIRNAAHQADTGPLDPACACYTCRNFSRSYLRHLDRCNEILGA
                     RLNTIHNLHYYLELMRRLRAGIESGSLDTVIREYVREAPDPAQSSVA"
     gene            3773..4105
                     /gene="yajC"
                     /locus_tag="VIV63_01705"
     CDS             3773..4105
                     /gene="yajC"
                     /locus_tag="VIV63_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005527555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY6123338.1"
                     /translation="MNSLIPDAMAQAAGGAPQGGGLSMFVMMGLFVVIFYFLLIRPQQ
                     KKQKEHQAMLAKLAAGDEVVTSGGIVGRITEITDNFVTIEISEGVRIKVQRFHVTSLV
                     PKGTLKGA"
     gene            4114..5994
                     /gene="secD"
                     /locus_tag="VIV63_01710"
     CDS             4114..5994
                     /gene="secD"
                     /locus_tag="VIV63_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY6123339.1"
                     /translation="MLEYARWKYILVAAVLAVAMVFALPNVFGDDYALQIARKDRAAI
                     EQTQLATIESTLKNAGVAFTSVELDKGNVTVRFADNETQLKGRDIVKDEKTGLAKDYV
                     NAMMYASRAPRWIQALGLRAMPLGLDLRGGLYLLYQVDVDGAVSGLLDSYDQDFRRTL
                     GIDKIAYNDISILTVDSDIPNGLRILLPAGADRSAVRAAIKKVQPDLGCRDADVAGGP
                     AVDCVMTAQQVRERRDFAITSNITTLRNRVNELGVSEPIVQRQGLDRILVQLPGVQNS
                     AEVKDILGKVATLEYRLVDPRPIPPSGRAPLGAKIYDRVDGGQYIGKVMLKRDIIVTG
                     NQLVNATSSTGQSGPQVDVTLDSAGGAEMLKTTQANLGKPMAVVFIEQRSETTGEGVE
                     QVTRNIKEEKVISVATINGVFSNRFQTTGLTMAEARETALLLRGGALAAPISIVNERS
                     IGPQLGKANIEKGVRALVVGMLALFAFMILYYQLFGVVANLVLIANVVLLSALLSMLG
                     TALSLPGIAGIILTVGMAVDANVLIYERIREELRNGVSPQAAIKAGFEKAFSAIADSN
                     VTTFIAGVVLFVFGTGAIKGFAIVLSLGILTSMFTALMGSRALLTLMFGGKRKVAKLA
                     IG"
     gene            6008..>6410
                     /locus_tag="VIV63_01715"
     CDS             6008..>6410
                     /locus_tag="VIV63_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123340.1"
                     /translation="MEFFHKKTSFPFMATRKVWYTLSAILMIVSFASFFTRGLNFAID
                     FTGGISVEASFVKDAPIDTIRSSVEEAGYHEPQVQILGSTRDVTIRLQSTGETAEVLR
                     PKFQAILSKIDPQVVISDLAVVGPQVGDELRY"
BASE COUNT         1130 a         2138 c         2050 g         1092 t
ORIGIN      
        1 aaggatgcgg agctgatctc gcgcctgcgc cggctcgatg ccgccgctgc gacggcggag
       61 ccgggctttg gctacgaagg cctgatcggc cggcatgccg aaaagcagaa ccgcgctcgc
      121 cgccgtcatt cgttcgcgcg cggcacggcc agcctgctgg tcgtcgccat ggtgggcgtc
      181 ggcgtctggc gtttcgagca ggaggagggc gtgagaaccg tcgaggtcat cgcgccggaa
      241 gtcgcgagcg acagccgcgc cgcgcagccg cgcatcgtgc gcgccgacac ctatttcgcg
      301 gtcgccgcgc tcgaggacca cattgccagc atcgacgatg cgctcaacga cgcgcgcgcg
      361 gcgcgcggca ccgccgaggt cgcgcgcctg gagcgcaccc gcgagctgct ggtcgattca
      421 tacacgcgtg tgcgttacgc cgagatggtg aacgccaatt tttgaggaac cgtcatgaaa
      481 aagctgatgc tgatttgttt gttagggctg agtgccgcgg cgaccgcgca acagaccaag
      541 agcgtggatg cgcaggtctc cagggatgag gcccggcgcg acgcggagga agcgaagacg
      601 cgcgccaaac tcgacgaggc gcgtacgcgg ctcgacaagg ccgcgcgcga tgtcgcggag
      661 ctctcgaacc agctcggggc gaacgtgcgg cacgacatcc gtatcgtcga gggcggtccg
      721 cgcaaggccg tgctcggaat ccaggtggat ccggacagcg gcagtgacgg agtgcgtgtg
      781 ctcagcgtga gcccgggagg acctgcggcg gaggccggtc tccgtgatac cgacgtcatc
      841 gtttcgctca atggcgtggc gacgatcggc ggcgatcccg accgcacgtt gatcacccag
      901 atgcgcacgg tcaaacccga acagaaagtg aaggtgcggg tgttgcgcga cggcaagaac
      961 aaggacttca ccgtggtcgc ccggcccatg gctttcgacg agcgcaggtt caacgtgcgt
     1021 ctgcccgaag gcggcgccat gggcgtcaat atgcccatgg tgcagcactt ccgcgcgttc
     1081 tttcccggcg agttcggtgg catggagctc gcgagcatca cgcccaggct cggcgcgtat
     1141 ttcggcgtga ccgaaggcgt gctcgtggtg caggcacccg agaacgacgc gttcaagctc
     1201 gaagacggcg acgtgctcca ggccatcgac ggccgcaagc ccgaagacgg cggacacgcg
     1261 atgcgcatcc ttcgctcgta caagtcgggc gagaaactca atctcacggt gttgcgccag
     1321 cgcaagccgg tcacgctcgc ggtgaccatg ccggaacgtc cggagttcgg cgacaacatg
     1381 atgttcggca cggggccggc gccgctcatg ccgcctgtcc caccggcgcc gccggtgccg
     1441 ggtacgccgg gatcgggcgc gggcactttc tgaagaagat agcggccatg gcggtaaact
     1501 cgccgccgtg cgccgctccg acttcaccta cgacctcccc gaagtactca tagcccagca
     1561 accgctcgcc gagagatcgg cgagccggct gctgacgctc gatggcgcca ccggcgcgct
     1621 cgcggaccgg cagatgcgcg acctgcccga gctcgtgtcg ccgggcgacc tgctgatatt
     1681 caacgatacg aaggtgattc ccgcgcgcct gttcgcgatc aaggaatccg gcggcaaggt
     1741 ggaattgctg ctcgaacgtc cgctcgattc gagatccgcg ctggtgcacg cgcgcgccag
     1801 caaaccgctg cggccgccca tgacgctgca gggccgcggt ggccgcatcg aggtcgtcga
     1861 aaaacgcggc gacctgtggg tgatcgaact accggaagcg ccgctcgcct tcttcgagcg
     1921 ttacggccag atgccgttgc cgccgtacat ccggcgcgag cccgatgccg ccgacagcac
     1981 gcgttatcaa agtgtcttcg cgaagcggca gggcgccgtc gcggctccca ctgccagcct
     2041 gcacttcgac gagccactgc tggcggcgct caccggacgc ggcgcgcatg tcgccttcgt
     2101 cacgctgcac gtgggtgcgg gcacgtttca gccggtgcgg accgatgcgg tcggcgcgca
     2161 cgtgatgcac gcggaattcg tcgaggtgag cgacgcggcc gtcgcggccg ttgccgccgc
     2221 gcgcgcacgc ggagggcgcg tcatcgccgt gggcaccacc gtcgttcgcg cgctcgaaag
     2281 cgccgcgggc ggcggatcgc tgtcgccgta catcggcgat tcatcgttgt tcatcgtgcc
     2341 cggctatcgt tttaacgtgg tcgatgcgat ggtgaccaac ttccatctgc ccgaatccac
     2401 gctgctcatg ctggtgagcg cgttcgccgg gcgcgaggcc gtgctcgccg cctatcggca
     2461 cgccgtcgcc gggcagtacc gttttttcag ttatggtgac gccatgttcg taacgcccgc
     2521 gctgaacgcg cacgcatgac cactcgagaa tgacagccga gttcactttg ttaggcaccg
     2581 cggggctcgc gcgccgcggc cggttgaagc tcgctcacgg caccgtggag acgcccgcat
     2641 tcatgccggt cggcacctac ggcacggtca aggccatgac tcccgaagag ctcgaaggcc
     2701 tcggcgcgga gatcgtgctc ggaaacacgt tccacctgat gctgcgtccg ggaaacgaag
     2761 tcctgcgcgc gcatggcggt cttcatggat tcatgaactg gcgccgcccg atcctcaccg
     2821 attccggcgg ctttcaggtc ttcagcctgg aatcgctgcg caagatcacc gaagagggcg
     2881 tgcagttccg ctcgcccatc gatggcagcg cggtgcggct gacgcccgaa gattcgatgg
     2941 acacgcagct cgcgctgggc tcggacatcg cgatggcgct cgacgactgc acgccgtatc
     3001 cggccaccga acaacaggcg cgcgattcga tggagcgttc gatgcgctgg gccgcgcgca
     3061 gccacgcgca ttactaccgc ccggatgccc atggcaacgc gccgcccggc gagttgtttg
     3121 cgatcgtgca gggcggcatg tttctgccgc tgcgccacgc ctcgctcgaa accctgcaga
     3181 aacttccgtg gaaaggattc gcggtaggtg gactggcggt gggcgaaccc gaagaggacc
     3241 gcctgcgcgt gctcgaggga ttgctgccgc acatgcccgc cgacaagccg cgttacctga
     3301 tgggcgtcgg ccggccccag gacatcatcg ccgcggtggc ccgcggcatc gacatgttcg
     3361 attgcgtcat gcccacccgg catgcgcgca atggccacct gttcacctca caaggtgtgg
     3421 tcaacatccg caacgccgcg caccaggcgg ataccggtcc gctggacccg gcctgcgcct
     3481 gctacacctg ccgcaatttt tcgcgttcct atctgcgcca cctggaccgc tgcaacgaaa
     3541 tcctcggtgc ccggctcaat accatccaca accttcatta ctacctggaa ctcatgcgcc
     3601 ggctgcgcgc gggcatcgaa tcgggctcgc tagacacagt gattcgcgaa tacgtgcgcg
     3661 aagcgccgga ccccgcccag tcttcagtgg cataaattta tttttcggcc aaaatacgcg
     3721 gccgcgccgc cccgaggcgg cgccgttttt tcgtcacgcg tgagttaaca ccatgaattc
     3781 attgatcccc gacgccatgg cgcaggccgc tggcggagcg ccccagggcg gcggcctctc
     3841 catgttcgtg atgatgggcc tgttcgtcgt catcttttac ttcctgctca tccgtccgca
     3901 gcagaagaag cagaaagagc accaggccat gctggccaaa ctggccgcgg gcgacgaagt
     3961 cgtcacctcc ggcggcatcg tcggccgcat caccgagatc accgacaact tcgtcaccat
     4021 tgaaatctcc gaaggcgtgc gcatcaaggt ccagcgtttc cacgtcacct ccctggtgcc
     4081 gaagggcacg ctcaagggag cctgaggtct tacatgctcg agtacgcgcg ctggaaatac
     4141 atcctcgtcg ccgccgtgct ggcggtggcg atggtcttcg ccctgcctaa cgtgttcggc
     4201 gacgactacg cgctgcagat cgcgcgcaag gatcgcgccg ccatcgagca gacgcagctc
     4261 gcgaccatcg agtccacact caagaacgcc ggcgtcgcct tcaccagcgt cgagctcgac
     4321 aagggcaacg tcacggtccg cttcgccgac aacgagacgc agctcaaggg ccgcgacatc
     4381 gtcaaggacg aaaagaccgg tctcgccaag gactacgtga acgcgatgat gtacgcgtcg
     4441 cgtgcgcctc gctggatcca ggcgctgggc ctgcgcgcca tgcctctggg tctcgacctg
     4501 cgcggcggtc tctatctact gtaccaggtg gacgtcgacg gggccgtctc cggcctgctc
     4561 gattcctacg accaggattt tcgccggacg ctgggcatcg acaagatcgc ctacaacgac
     4621 atcagcatcc tcacggtcga ctccgacatt ccgaatggtc ttcgcatact gctgcccgcg
     4681 ggtgcggatc gcagcgcggt gcgcgccgcc atcaagaagg tgcagcccga tctcggctgc
     4741 cgcgatgccg acgtcgccgg tggcccggcc gtcgattgcg tgatgaccgc gcaacaggtg
     4801 cgcgagcgcc gtgacttcgc catcacctcc aacatcacca cgctgcgcaa ccgcgtcaac
     4861 gaactcggcg tgtccgaacc catcgtgcag cgccagggcc tcgatcgcat cctcgtgcag
     4921 ctgcccggcg tgcagaactc ggccgaagtg aaggacatcc tcggcaaggt cgcgacgctc
     4981 gaatatcgcc tggtcgatcc gcggcccatt cccccgagtg gccgtgcgcc gttgggcgcg
     5041 aagatctacg accgcgtcga cggtgggcaa tatatcggca aggtcatgct caagcgcgac
     5101 atcatcgtca cgggcaacca gctcgtgaac gccacgtcga gcacgggcca gagcggtccg
     5161 caggtcgacg tgacgctcga cagcgccggt ggcgcggaaa tgctcaagac cacgcaggcc
     5221 aatctcggca agcccatggc cgtggtgttc atcgaacagc gcagcgaaac caccggtgaa
     5281 ggcgtcgagc aggtcacgcg caacatcaag gaagagaagg tcatcagcgt cgcgaccatc
     5341 aacggcgtgt tcagcaaccg tttccagacc accggcctca ccatggccga ggcgcgcgag
     5401 accgcgttgc tgttgcgcgg tggcgcgctg gcggcgccca tctccatcgt caacgaacgt
     5461 tcgatcggcc cgcagctcgg caaggccaac atcgagaagg gcgtgcgcgc cctcgtggtc
     5521 ggcatgctcg cgttgttcgc gttcatgatc ctctactacc agctgtttgg cgtggtcgcg
     5581 aacctcgtgc tgatcgcgaa cgtcgtactg ctgtcggcgt tgttgtccat gttaggcacc
     5641 gcgctgtcgt tgccgggaat cgccggcatc atcctcaccg tcggcatggc ggtcgacgcc
     5701 aacgtgctga tctacgaacg catacgcgaa gaactgcgca acggcgtatc gccgcaggcg
     5761 gccatcaagg ccggcttcga gaaggcgttc tcggccatcg ccgactccaa cgtcaccacg
     5821 ttcatcgcgg gcgtcgtgtt gttcgtgttc ggcaccggcg ccatcaaggg cttcgccatc
     5881 gtgctgtcgc tcggcatcct cacgtcgatg ttcacggcgc tcatgggcag ccgcgcattg
     5941 ctcacactga tgttcggcgg caaacgcaag gtcgccaaac tggcgatcgg ctgagaggaa
     6001 tcgcaccgtg gaattttttc acaagaagac cagcttcccg ttcatggcca cacgcaaggt
     6061 gtggtacacg ctttcggcca ttctcatgat cgtgagcttc gcgtcgttct tcacgcgtgg
     6121 cctgaacttc gcgatcgact tcaccggcgg catcagcgtc gaggcgagct tcgtgaagga
     6181 cgcgcccatc gacacgatcc gctcctccgt cgaggaagcc ggttatcacg agccgcaggt
     6241 tcagatcctg ggttcgacgc gcgacgtcac catccgactg cagtccacgg gcgaaaccgc
     6301 cgaagtactg cggccgaaat tccaggcgat tctgtcgaag atcgatccgc aggtcgtgat
     6361 cagcgacctc gccgtcgtcg gtccgcaggt cggcgatgag ctgcgttact
//
LOCUS       DATDEU010000075        10789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_13243, whole genome shotgun sequence.
ACCESSION   DATDEU010000075 DATDEU010000000
VERSION     DATDEU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13243"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VIV63_01720"
     CDS             <1..555
                     /locus_tag="VIV63_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY6123341.1"
                     /translation="RARPGGLRLDVGRWREFAAGVAVGLSLSLFVLIWQNYREKKVDA
                     EVAAVPKPEPRADKAAAGDAEEGAKNYDFYDMLPKFEVVVPEKEREVASSDRGAAPAN
                     IERPGVYVLQAGSYRQQSDADRVRAQLKMQGIDANVQRVAVDEDVWHRVRIGPITDLT
                     ELNRLRTRLRAADFDDALVIRVGD"
     gene            569..1690
                     /locus_tag="VIV63_01725"
     CDS             569..1690
                     /locus_tag="VIV63_01725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY6123342.1"
                     /translation="MSRPGILAGAALLALLAACATEPPAKPAPPPAPTAPAAPATPPI
                     PPPTHPGLTLAAVGDMMLGTDFPENILPDDDGLSFFDAVTPILDKPDVTFGNLEGVLQ
                     DGGEPVKQCKNRKICFLFRTPARYATYFKLAGFDVLSLANNHARDFGEEGRSSSMAAL
                     DAAGIHHSGREGTVASFIANGRKIAMVAFAPNVGSNSLNDPQIGLPLVADMATKHDIV
                     IVSFHGGAEGSGAEVMPFAREIFAGEDRGNVVEFGRAMIDAGADIVIGHGPHVVRPLE
                     IYKDRLIAYSLGNFATYYGISVEGIRGIAPILLVTLDDEGKFVSGRIEPTIQIRPAGP
                     APDPAKGVIKLLQSLTTTAFPAGELSITDDGALVRRSPP"
     gene            1687..2235
                     /locus_tag="VIV63_01730"
     CDS             1687..2235
                     /locus_tag="VIV63_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123343.1"
                     /translation="MSRWLIALVCCQLTACATSLVPITAAEGRQSDDQLLGRWLSTLE
                     DETEKISIVKRDGELIVIGENAEGPTLGSEQTRVIVAKIDGQRYASFTDIEAASNEYP
                     RKYMLARYRFVDADHVVVYGPNIDLLMAAVNRQQIAGTELKDRHMAGVELSATAEQLR
                     DYVSAEGVRIFSLKLFQLERLK"
     gene            complement(2251..3618)
                     /locus_tag="VIV63_01735"
     CDS             complement(2251..3618)
                     /locus_tag="VIV63_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6123344.1"
                     /translation="MSAAHILVVDDEADIRGLLKEILSEEGYDVDVAGDAGQARASRA
                     RQLPDLVLLDIWMPDTDGITLLREWQGASAEVSCPVVMMSGHGTVETAVEATRLGAFD
                     FVEKPLSLQKLLRTVERALEAGKRKRHAGKLLAASPGVPVGRSRVMQQLRADLAQIAT
                     NDSPVLLVGETGTGREAFARFIHDASPRAPRPFVTLISSTLRGEGADQVLFGTEAGGQ
                     IAPGVLERAGAGTLFINEIEDLPPQSQRSLLGTLESGQFTRVGGNVPVTFSARMLSSA
                     RPGIEGRADEFRRDLLAHMNTLIVRVPPLREYTEDVPDLLRHYVDRMVDGEGLQFRKF
                     SVAAQNRLRNYPWPDNVRELKNLVQRLLLQGGGEEIRLEEIERELAAQAPSVEPLVKQ
                     DLLALPLREAREQFERAYLQQQLLLCNGKVGQLAKRVGMERTHLYRKLRSLGVDFRNI
                     TDDAE"
     gene            complement(3615..5861)
                     /locus_tag="VIV63_01740"
     CDS             complement(3615..5861)
                     /locus_tag="VIV63_01740"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6123345.1"
                     /translation="MVPAALRRFGTLGLIALWVLVSGGLLLLLALSVQNSMQFSRLQA
                     WILLANVIALIAVSVLLARKLIELVRAFRAGVPGSRLTARTVAIFGSLVVAPLIIVYL
                     FSLEFLNQGIDSWFKVEIKQGLTDALDLSKSAMDVRMREYARSTQLFANRLADSPPSN
                     WAEATDAQRAESGALEVVVYGTLGQILAASSAGSGSTNLPRASAEVTSAISAGNVYWS
                     LEPLSEDDYLINTAAPIATGPGSSQGRFVVAQYPVPPQLSRLSEAVQHSYSQHYNLTQ
                     QREGVKNLFGMTLTLVLLLAMLTAIYGAIYSAEKLTRPVQDLIAGTRAVGKGDFGTRL
                     PLPSRDEMGFLVHSFNDMTKRLRRAREETTRSQMAVERERERLSIILARLSTGVIAIG
                     RDLVLRSANQAAGNILGANFEQGVGQPLSALDAENPRYQRFVGEVTMRLARGEEEWRE
                     QLDLKPEAAGARVLVCACTPLPGDEHAEDDASAQGGYVIVFDDISALLQAQRDAAWGE
                     VARRLAHEIKNPLTPIQLSAERMRRRYLAQMTGEDAQILDRATYTIVQQVEAMKAMVN
                     AFSEYARAPDMKVTTFPLNALVTEVADLHRVQESTLRIDVDLDPRIENVEADRGRVRQ
                     ILNNLIVNGAEAVENSPAGHVVASTRLESVGSAEYATITVTDNGPGFSRDVLGRVFDP
                     YVTSKPKGTGLGLAIVKKIVEEHGGRVEADNRPEGGGRVRVSLPVTDGSRQMVARDRR
                     AEPRKERA"
     gene            complement(5843..6433)
                     /locus_tag="VIV63_01745"
     CDS             complement(5843..6433)
                     /locus_tag="VIV63_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEY6123346.1"
                     /translation="MQFRLAHNLFTLLLAALVGVGAGRVARADVLDGLLEVRSAYVTE
                     GQGVFQLFARVAYPVNDDIRAALKDGLMLNFDLDVVVSRERRFWVDESVAEYTLRREL
                     LYHAVSDRYVARDVDSRNSEQHSYSTLEEALESLGTVEAWPFLLSPQLSPNRQYRVSL
                     RAGVRRGRQSDTLRVLMFWTDDWHRESEWFSWSLQR"
     gene            complement(6454..7821)
                     /gene="rsmB"
                     /locus_tag="VIV63_01750"
     CDS             complement(6454..7821)
                     /gene="rsmB"
                     /locus_tag="VIV63_01750"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY6123347.1"
                     /translation="MKKVRHNPPPQPRRTLHAPGAETLAAAALAVHDVVYEGRSADAT
                     LEATRERQDRAAVRAIALGTLRWYLRLQPALAPLVSRPFDELSPRLAALLVTTAHQVE
                     YSRGAAEAQVHLAVDASRVLGEGRASGVVNAVLRKFVAQRVELLAAADATLPQQHAHP
                     QWLVEALRKAWGERAAAILEANNQHPPMVLRLDPALISSDDFLRSLRALGRDASVVGW
                     NRDAIVLDRPAAVQTLPGFETGAVSVQDAGAQLAAPLLDAQPGMSVLDACAAPGGKTL
                     HIAQRTPGLAELIAIDDDPLRLARVRENLERAGRDALLVTADLRSMPPSLAPGRFDRV
                     LVDAPCSATGVIRRHPDIKHLRRPGDIDGFVATQKQILATAFELLKAGGRLTYCTCSV
                     IPAENEGVVGAFINSQPRAAIAPWPEGLVRPPDLLDRPFGWQLLPGGSAGTDGFYYAC
                     LTKLS"
     gene            complement(7818..8759)
                     /gene="fmt"
                     /locus_tag="VIV63_01755"
     CDS             complement(7818..8759)
                     /gene="fmt"
                     /locus_tag="VIV63_01755"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY6123348.1"
                     /translation="MTSLRIAFAGTPQFALPALRALLKSAHRVVGVLTQPDRPAGRGQ
                     HLRASPVKLLATEHGLPVAQPATLKTPEGRAELAQWAPDVLVVVAYGLILPQPALDLP
                     RLGCVNIHGSLLPRWRGAAPIHRAILAGDAETGVTIMQLDAGLDTGPMLLERSRPIGT
                     NDTAGDLHDALSELGAVALIEALDGLARGTLRPRAQPAAGATYAPKIEKTEATLDWSA
                     SAVTLDRKVRAFNPWPIAETRFGDETLRILRARVAEPGGVHAGPGTLLGIADDGLRVA
                     CGEGVLAISELQRAGKRPITARDFANAVRVAGLRFGT"
     gene            complement(8796..9335)
                     /gene="def"
                     /locus_tag="VIV63_01760"
     CDS             complement(8796..9335)
                     /gene="def"
                     /locus_tag="VIV63_01760"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY6123349.1"
                     /translation="MARLNILEYPDPRLRTKAKPVEVFDAALARLAQDMLETMYDAPG
                     VGLAATQVDVHKRLIVMDVSEGKKDPQVFCNPLILTREGVEVTEEGCLSVPGIFDEVK
                     RAATIRARAQDVTGKVFEVDLDGLAAVCLQHEMDHLEGKLFVDYLSDLKRERIRKKLE
                     KDRKERAGKARESSSARAY"
     gene            9552..10757
                     /locus_tag="VIV63_01765"
     CDS             9552..10757
                     /locus_tag="VIV63_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY6123350.1"
                     /translation="MLAILNPRVIALMALIAAALVAAGCKSAPPPVTQPISTPPPAAQ
                     ASEPVAEDLTATEAAVAALNEAPVAETSAPTAQLIRPDAPMNYTVKRGDTLWDIAAVF
                     LKDPWFWPEIWQINPQVENPHLIYPGDVLSLAYGANGDASVRISQYSGARLQPRLRSE
                     ALDGPVENIPFSAIAAFLTRPSVLTKEQALAAPHILAFRDQHMIGGTGHEAYVRNLNA
                     PVDQRFLVMHVGDPIRDIDSNDILGYQAAYVATAVVETTAEISKTKLTDGAREALAGD
                     RLIIEEGERSLNFQPHAPATAVDGQIIAVADGAEQVGPYQVVVLNRGAEAGLTQGAVL
                     AVDQQGELIVDKSRHYPWKKEAFPDEVRLPYERAGTLIVFKVYEKISYGLVIGARAPM
                     RVADRVYNP"
BASE COUNT         1869 a         3507 c         3575 g         1838 t
ORIGIN      
        1 cgcgcccgcc ccggcgggct gcgactcgat gtcggccgct ggcgggaatt cgccgcgggc
       61 gtcgcggtgg gcctgtcact ctcgctgttc gtgttgatct ggcagaacta tcgcgagaag
      121 aaagtggatg ccgaggtcgc cgccgttccc aagcccgagc cgcgcgccga caaggccgcc
      181 gcgggcgacg ccgaggaagg agccaagaac tacgacttct acgacatgtt gcccaagttc
      241 gaagtggtgg tgccggagaa ggaacgcgag gtggccagca gcgatcgcgg cgcggcgccc
      301 gcgaacatcg aacgccccgg cgtctacgtg ctgcaagccg gttcgtatcg ccagcagtcg
      361 gatgccgacc gggtccgcgc gcagctcaag atgcagggca tcgacgcgaa cgtgcagcgc
      421 gtggcggtgg acgaagacgt gtggcaccgc gtgcgtatcg gccccatcac cgatctcacc
      481 gagctcaacc ggctgcgcac gcgcctgcgc gccgccgatt tcgacgatgc gctggtcatc
      541 cgggtgggag actagttaga tgacggcaat gtcgcgtccg ggaatcctgg ccggcgcggc
      601 cttgctcgcc ctcctggccg cgtgcgccac cgagccgccc gcaaaacctg cgccgccccc
      661 ggcgccgacc gctcctgcgg cgcccgccac accgcccatt ccgccgccga ctcatccggg
      721 actcacactc gcggccgtcg gcgacatgat gctgggcacg gattttcccg agaacatcct
      781 gcccgacgac gacgggttgt cgttcttcga cgcggtgacg ccgattctcg acaagcccga
      841 tgtgaccttc ggcaacctcg agggtgtgct gcaggacggc ggcgagcccg tgaagcagtg
      901 caagaacagg aagatctgtt tcctgttccg cacgccggct cgttacgcca cgtatttcaa
      961 gctcgcaggc ttcgacgtgc tgagcctggc caacaaccac gcgcgtgact tcggcgaaga
     1021 aggccgctcg tcgagcatgg ccgcgctcga cgccgcgggc attcatcatt cgggacgcga
     1081 aggcacggtc gcgagcttca ttgccaatgg ccggaagata gcgatggtgg cgttcgcgcc
     1141 caacgtcggt tcgaactcgc tcaacgatcc gcagatcggt ctgccgctgg tggccgacat
     1201 ggccacgaag cacgacatcg tcatcgtgtc gtttcatggc ggggcggaag gcagcggcgc
     1261 cgaggtgatg ccgttcgcgc gcgagatctt tgccggcgaa gaccgcggca acgtggtcga
     1321 gttcgggcgc gccatgatcg acgccggcgc ggatatcgtg atcggccatg ggccgcacgt
     1381 cgtgcggccg ctggaaatct acaaggaccg gctgattgcc tacagtctcg gaaatttcgc
     1441 gacctactac ggcatcagcg tcgaaggcat ccgcggcatt gcgccgatcc tgctggtgac
     1501 gctggatgac gaagggaagt tcgtttcggg acgcatcgag ccaacgatcc agatccgccc
     1561 ggccggcccg gcgccagacc cggcgaaggg cgtgatcaag ctgctgcagt cgctaactac
     1621 caccgctttc cccgcaggcg aactgtcgat caccgacgac ggcgcgctcg tcaggaggag
     1681 cccgccttga gccgatggct gatcgcgctc gtttgttgtc agctgacggc atgcgcgacc
     1741 agcctcgtgc cgatcaccgc ggccgagggc aggcaatccg acgaccaact gctcggccga
     1801 tggctgagca cgctcgaaga tgaaaccgag aagatctcga tcgtgaagcg cgatggagag
     1861 ctgatcgtca tcggggaaaa tgcggaaggg ccaaccctcg ggtcggagca gacgcgggtg
     1921 atcgtcgcga agatcgatgg ccagcgttac gccagtttca ccgacatcga ggctgcatcg
     1981 aacgagtatc cccgcaagta catgctcgcg agatacaggt tcgtcgatgc cgaccacgtg
     2041 gtcgtatacg gcccgaacat cgacctgctg atggcagcgg tgaaccgcca gcagattgcc
     2101 ggcacagaac tgaaagaccg gcatatggcc ggcgtggaac tgagcgcaac ggccgaacaa
     2161 ttgcgcgact acgtcagcgc ggagggcgtt cgcattttca gcttgaagtt attccagctc
     2221 gagcgcctga agtagcgcgc cgggcggaag ctattccgcg tcgtcggtga tattgcggaa
     2281 gtcgacgccc agcgatcgca gcttgcggta cagatgcgtg cgctccatgc cgacgcgctt
     2341 ggcgagttgc cccaccttgc cgttgcacaa caacagctgc tgctgaagat aggcgcgttc
     2401 gaattgttca cgcgcttcgc gtagcggcag cgccagcagg tcctgcttga ccagcggctc
     2461 gacgctcggc gcctgcgcgg ccagctcacg ttcgatttcc tcgaggcgaa tttcttcacc
     2521 gccgccctgc aacaacaggc gctgcacgag attcttgagc tcgcgcacgt tgtcgggcca
     2581 cgggtaattg cgcagccggt tctgggcggc gacgctgaac ttgcggaact gcagaccttc
     2641 gccgtccacc atgcggtcga cgtaatggcg cagcaggtcg ggcacgtctt cggtgtattc
     2701 gcgcagcggc ggcacacgca cgatcagcgt gttcatgtgc gcgagcagat cgcgacggaa
     2761 ttcgtcggcc cggccttcga tgccagggcg cgcggacgac aacatgcgcg cactgaacgt
     2821 gaccggcaca ttaccgccaa cgcgcgtgaa ctggccgctt tcgagcgtac cgagcagcga
     2881 gcgttgcgat tgcggcggaa gatcttcgat ttcgttgatg aacaacgtac cggcgcccgc
     2941 gcgttcgagc acaccgggag cgatctgccc gccggcctcg gtgccgaaca acacctggtc
     3001 ggcgccttcg ccgcgcagcg tgctggaaat gagcgtgacg aacggccgcg gggcgcgcgg
     3061 gctggcgtcg tggatgaagc gcgcgaaagc ttcgcggccc gtgccggtct cgccgaccag
     3121 cagcacgggg gaatcgttgg tggcgatctg cgcgagatcg gcgcgcaact gctgcatgac
     3181 gcggctgcgg cccacgggca cacccgggga agcagctaac aacttgccgg cgtgacgctt
     3241 gcgcttgccc gcctccagcg cgcgctcgac cgtacgcagc agtttctgca gcgacagcgg
     3301 cttctcgacg aaatcgaacg caccgagacg ggtggcctcg acagcggttt cgacggtgcc
     3361 gtgcccggac atcatgacga cggggcagga gacttccgcg gacgctccct gccactcgcg
     3421 cagcagcgtg atgccgtcgg tgtcgggcat ccagatgtcg agcaggacga ggtcgggcaa
     3481 ctggcgtgca cgcgaggcac gcgcctgccc ggcgtccccg gcgacgtcca cgtcgtaacc
     3541 ctcctcggaa agaatttcct tgaggaggcc tcggatgtcg gcttcatcgt cgacaaccag
     3601 aatgtgcgcg gcgctcatgc tcgctccttc ctgggttctg cgcgtctgtc gcgcgcaacc
     3661 atctgtcggg atccgtctgt taccggcaag gatacacgca cgcgcccgcc gccttccggc
     3721 cgattgtcgg cttccacgcg cccgccatgc tcttcgacaa tctttttcac gatcgcgagc
     3781 cccaaacccg tgcctttggg cttgctggtc acgtaggggt cgaacacgcg ccccaataca
     3841 tcgcgcgaaa acccggggcc gttgtcggtg acggtaatgg tggcgtactc ggcgctgccg
     3901 acgctctcca gccgcgtcga ggcgaccacg tgcccggccg ggctgttttc caccgcttcc
     3961 gcgccgttga cgatgaggtt gttgaggatc tggcgcacgc gcccgcggtc cgcctcgacg
     4021 ttttcgatgc gcgggtcgag atcgacgtcg attctcagcg tcgattcctg cacgcggtgc
     4081 aggtcggcca cctcggtgac cagcgcgttg agcggaaacg tcgtgacctt catgtccggc
     4141 gcacgtgcgt attcactgaa cgcgttcacc atggccttca tcgcttcgac ctgctgaacg
     4201 atggtgtacg tggcgcgatc gaggatctgc gcatcttcgc cggtcatctg cgcgagatac
     4261 cggcgccgca ttctctccgc ggacaactgg atgggcgtga gcgggttctt gatctcgtgt
     4321 gcgagccggc gcgcgacctc gccccaggcc gcgtcgcgct gcgcctgcag cagcgcgctg
     4381 atgtcgtcga acacgatgac gtaaccgccc tgggcagaag cgtcgtcttc ggcgtgttca
     4441 tcgccgggca acggtgtgca ggcgcacacc agcacgcggg caccggccgc ttcgggtttg
     4501 aggtcgagct gctcgcgcca ttcttcctcg cccctggcga ggcgcatggt gacttcgccg
     4561 acgaaccgct ggtaacgcgg attttcggca tccagcgccg agagaggctg gccgactccc
     4621 tgttcgaaat tggcgccaag aatattgccg gccgcctgat tggcgctgcg aagcaccagg
     4681 tcgcgcccga tggcgatgac gcccgtcgac aggcgcgcca ggatgatgga gagccgttcg
     4741 cgttcgcgtt ccaccgccat ctggctgcgc gtggtttcct ctcgcgcgcg ccgcaggcgc
     4801 ttggtcatgt cgttgaacga atgcacgagg aatcccattt cgtcgcgcga cggcagcggc
     4861 agccgcgtgc cgaagtcgcc cttgccgacc gcgcgcgtac cggcgatgag atcctgcacc
     4921 ggcctcgtga gcttctcggc cgagtagata gcaccataga tcgcggtgag catcgccagc
     4981 agcagcacca gtgtgagggt catgccgaac aggttcttca caccctcgcg ctgctgggtg
     5041 aggttgtagt gctgcgaata ggagtgctgc acggcttccg acaggcgcga gagttgcggc
     5101 ggcaccgggt actgcgccac cacgaagcga ccctggctcg aacccgggcc ggtcgcgata
     5161 ggcgcggccg tattgatgag gtaatcgtcc tccgacagcg gctccaggct ccagtacacg
     5221 ttgcccgccg agatggcgct ggtgacttcg gcggaagcgc gcggcaggtt ggtggagccg
     5281 gagccggcac tgctggccgc gagaatctgg cccagcgttc cgtacacgac cacctcgagc
     5341 gcgccgctct cggcgcgttg cgcatcggtc gcttccgccc agttggacgg cgggctgtcg
     5401 gcaagccggt tggcgaacag ctgcgtgctg cgcgcgtatt cgcgcatgcg cacgtccatg
     5461 gccgacttcg acagatcgag cgcgtcggtc aggccctgct tgatctccac cttgaaccag
     5521 ctgtcgatgc cctggttgag gaactcgagc gagaatagat agacgatgat gagcggcgcg
     5581 accaccagcg acccgaagat ggcgaccgtg cgcgccgtca gtcgcgaacc cgggacgccg
     5641 gcgcggaacg cgcgcaccag ctcgatgagc ttgcgcgcca gcaacacgct gacggcgatc
     5701 agcgcgatga cgttggccag caggatccac gcctggagcc ggctgaactg catggagttc
     5761 tgcaccgaca gcgccagcag caagagcaga ccgccgctca ccagcaccca cagggcgatc
     5821 agccccaggg tgccaaagcg tctcagcgct gcagggacca tgagaaccac tcgctctcgc
     5881 ggtgccagtc atccgtccag aacatgagca cgcgcagcgt gtcggactgg cggccacggc
     5941 gcacgccggc acgcaggctg acgcggtatt gccggttggg tgagagctgc ggcgacagca
     6001 ggaacggcca cgcttccacg gtgccgagcg attcgagcgc ttcttcgagc gtcgagtagc
     6061 tgtgttgttc gctgttgcgg gagtcgacgt cgcgcgcgac gtagcggtcg ctgaccgcgt
     6121 gatagagcag ttctcgccgc agcgtgtatt cggcgaccga ctcgtccacc cagaaacggc
     6181 gttcgcgcga aacgacgacg tcgaggtcga agttcagcat cagcccgtcc ttgagcgcgg
     6241 cgcggatgtc gtcgttgacc gggtaggcaa cgcgggcgaa cagctggaag acaccctgcc
     6301 cttcggtcac gtatgccgag cggacttcga gcaggccatc gagcacatcg gcgcgcgcaa
     6361 cccggcctgc acccacgccc acgagcgcgg ccagcagcag agtaaagagg ttatgggcga
     6421 gccggaactg catcggttcc tctgggtggg ttgtcaggat agcttagtga ggcaggcata
     6481 gtagaagcca tccgtgcccg cgcttccgcc cggcagcaac tgccagccga agggccgatc
     6541 gagcagatca ggcggccgaa caaggccttc cggccacggc gcgatggccg cgcgtggctg
     6601 tgaattgatg aaagcaccga ccaccccttc gttctccgcc ggtatcaccg agcaggtgca
     6661 gtaggtgaga cgtccaccgg ccttcagtag ttcgaaagcg gtggcaagaa tctgcttctg
     6721 ggtcgccacg aagccgtcga tgtcgcccgg ccggcgcaag tgcttgatgt cgggatgacg
     6781 gcggatcacg cccgtcgccg aacagggagc atccaccagc acacgatcga atcgcccggg
     6841 tgccagtgac ggcggcatag agcgcaggtc ggcggtgacc agcaacgcat cgcgccccgc
     6901 gcgctccagg ttttcgcgca cgcgcgccag ccgcagcgga tcgtcatcga tggcgatgag
     6961 ttcggcgagc ccgggggtgc gctgcgcgat gtgcagggtc ttgccgcctg gggccgcgca
     7021 ggcgtcgagc acgctcatgc cgggctgcgc gtcgagcaac ggagccgcga gttgcgcgcc
     7081 ggcatcctgg acggacacgg cgccggtttc gaatcccggc agcgtctgca cggccgcggg
     7141 ccggtcgagc acgatggcgt cgcgattcca gccaaccaca ctcgcatcac gcccgagcgc
     7201 gcgcagtgat cgcaggaagt cgtcggaaga gatgagtgcg ggatcgagcc tgagcaccat
     7261 gggcggatgc tggttgttgg cctcgagaat cgcggccgcc ctttcgcccc atgccttacg
     7321 taatgcctcg accagccact ggggatgcgc atgttgttga ggcaacgtcg catcggccgc
     7381 ggctaacaac tccacgcgtt gcgcgacgaa tttgcgcagc acggcgttga cgacgccgct
     7441 ggcgcgcccc tcgccgagca cgcggctggc gtcgaccgcg agatgaacct gcgcctcggc
     7501 cgcaccgcgt gaatactcga cctggtgcgc ggtggtgacg agcagcgccg cgagtcgcgg
     7561 cgacagttcg tcgaaggggc ggctgaccag aggcgcgagc gcgggctgca ggcgcagata
     7621 ccagcgcagc gttcccagcg cgatggcgcg tacggccgcg cgatcctggc gttcgcgtgt
     7681 ggcctcgagc gtcgcatccg cggatcgtcc ttcgtagacg acgtcgtgga cggcgagtgc
     7741 cgccgcggcc agtgtttccg cgccgggcgc atgcagcgtg cggcgcggct gcggcggtgg
     7801 gttgtgacga acttttttca tgtaccaaac ctcaggccag cgacgcgtac ggcgttggca
     7861 aaatctcgtg ccgtgatggg ccgcttgccc gctcgctgca gctcgctgat ggcgagaacg
     7921 ccttcgccac aggcgacgcg caggccatcg tcggcgatac ctaacaatgt gcccgggccg
     7981 gcatggacgc cgcccggctc cgcgacgcgg gcgcgcagga tacgcagcgt ctcgtcaccg
     8041 aagcgggttt cggcgatcgg ccacggattg aacgcgcgca ccttgcgatc gagcgtaacg
     8101 gcactggctg accagtcgag ggtcgcttcc gtcttttcga tcttgggcgc gtacgtcgcg
     8161 ccggccgcag gctgcgcacg cggccgcagc gttcctcggg ccagtccatc gagtgcttcg
     8221 atgagcgcga ctgctccgag ttcggacagc gcgtcgtgaa gatcgcccgc ggtgtcgttc
     8281 gtgccgatcg gacggctgcg ttcgagcagc atcggaccgg tgtcgagacc cgcatcgagt
     8341 tgcatgatgg tcacgcccgt ttccgcatct ccggcgagta tggcccgatg gatcggcgcc
     8401 gctccgcgcc agcgcggcag cagcgagccg tggatgttca cgcaaccgag tcgcggcaaa
     8461 tccagcgcgg gctgcggaag aatcaatcca tacgcgacga ccacgagcac atccggcgcc
     8521 cactgcgcga gttcggcgcg tccttcgggg gtcttgagcg ttgcaggctg cgccaccggc
     8581 agcccatgtt cggtggccag tagtttgacc ggcgaggcgc gaaggtgctg gccgcggccc
     8641 gcgggccggt ccggctgcgt cagcactccg acgactcgat gcgccgactt cagcagcgcg
     8701 cggagagcgg gcagcgcaaa ttgcggcgtg cccgcgaagg cgattcgtaa cgaggtcatg
     8761 ggagcgcccg aaggcttgag tgattcgtcg aaggatcagt acgcgcgtgc ggaactggac
     8821 tcacgcgcct tgccggcacg ttccttgcgg tccttttcca gcttcttgcg gatgcgttcg
     8881 cgcttgaggt cggacaggta gtcgacgaag agttttccct cgaggtgatc catctcgtgc
     8941 tgaaggcaga cggccgcgag gccgtcgaga tcgacttcga aaaccttgcc cgtcacgtcc
     9001 tgggcgcgcg cccggatggt cgcggcgcgt ttcacctcat cgaaaatgcc cggcaccgac
     9061 agacagcctt cctccgtgac ctccacccct tcacgcgtga gaatcaaagg attgcagaac
     9121 acctgcgggt ccttctttcc ttcggatacg tccataacga tcaatcgctt atgaacgtcc
     9181 acctgggtgg ccgcgagtcc gacgccgggg gcgtcgtaca tggtttcgag catgtcctgc
     9241 gccagacggg ccagtgcggc atcgaacacc tcgaccggct tggccttggt gcgaagacgg
     9301 gggtccgggt actcaagaat attcaggcgc gccatgatgt tccgctcgat tgctgctaat
     9361 gtttgggcga ttcgaccgtg gacaaggatt gcgatgcgtg aggcgggtgt gaacccgtga
     9421 tccagcgcac aaacttgaca atagatcgta ggccctcgtg ctgcggcgga ttataacagt
     9481 ggggtccgct tgccccaccc aggattgagg agtggaggat gtctcttaat caacaactta
     9541 gggagccgcg cgtgctcgca attctgaacc cgcgggtcat agccctcatg gccttgatcg
     9601 ctgccgcgct ggtcgctgcc ggatgcaaat ccgcaccgcc gccggtgacc cagcccattt
     9661 cgaccccgcc gccggccgcc caggcctccg agccggtggc cgaagacctc actgccaccg
     9721 aagcggcagt ggccgccctg aacgaggcgc ccgttgcgga aacctctgcg cccaccgcgc
     9781 agctcatccg ccccgatgcg cccatgaact acaccgtgaa gcgtggcgac acgctgtggg
     9841 atatcgccgc ggtattcctc aaggacccgt ggttctggcc cgagatctgg cagatcaatc
     9901 cgcaggtcga aaacccgcac ctcatctatc cgggtgacgt gttgtcgctg gcgtatggcg
     9961 ccaacggcga tgccagcgtg aggatttcgc agtacagcgg cgcccgtctt cagccgcgac
    10021 tgcgcagcga agcgctcgac ggcccggtcg aaaacattcc gttctcggcc atcgccgcgt
    10081 tcctgacgcg cccgagcgtg ctcaccaagg aacaggcgct cgcggctccg catatcctcg
    10141 cgttccgtga ccagcacatg atcggcggta cgggccacga agcgtatgtg cggaatctga
    10201 acgctccggt ggatcagcgt ttcctcgtca tgcacgtcgg cgatccgatc cgtgacatcg
    10261 attcgaacga catcctggga taccaggccg cgtacgtcgc caccgccgtg gtggaaacga
    10321 cggctgaaat ctccaagacc aagctcaccg atggtgcgcg cgaagcgctc gccggcgacc
    10381 ggttgatcat cgaagaaggc gaaaggtcgc tcaacttcca gccgcacgcg cctgccacgg
    10441 cagtggacgg ccagatcatc gcggtagcgg acggcgccga acaggtcggt ccttaccagg
    10501 tggtggtgtt gaatcgcggt gcggaagccg gactcacgca gggtgcggta ctcgccgtcg
    10561 atcagcaggg tgagttgatc gtcgacaagt cccgccacta tccgtggaag aaggaggcgt
    10621 tcccggatga agtgcgcctg ccttacgagc gcgccggcac tctcatcgtg ttcaaggttt
    10681 acgagaagat cagctacggc ctggtcatcg gtgcacgtgc tccgatgcgc gtcgccgatc
    10741 gcgtttacaa cccgtaacgc atttctcttc attctgacga tttagggcc
//
LOCUS       DATDEU010000076         8485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_597033, whole genome shotgun sequence.
ACCESSION   DATDEU010000076 DATDEU010000000
VERSION     DATDEU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597033"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..336
                     /gene="rpsO"
                     /locus_tag="VIV63_01770"
     CDS             67..336
                     /gene="rpsO"
                     /locus_tag="VIV63_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY6123351.1"
                     /translation="MSLATEKKTGVVAQFQRATNDTGSPEVQIALLSERINGLGDHFG
                     AHKRDHASRRGLVKLVNQRRKLLDYLKATKPAKYQEVVERLGLRR"
     gene            434..2524
                     /gene="pnp"
                     /locus_tag="VIV63_01775"
     CDS             434..2524
                     /gene="pnp"
                     /locus_tag="VIV63_01775"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017391359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY6123352.1"
                     /translation="MSGLKKSFQYGQHSVSIETGEVARQASASVVVSMGDTVVLVTAV
                     AAKKAVPGKDFFPLTVNYIEKTYAAGRIPGGFFKREGRPTEKETLTSRLIDRPIRPLF
                     PDGFFNEVQVVAMVLSMDPDIDADIPSLIGASAALSLAGVPFAGPIAAARVGYKNGQY
                     QLNPTQKELKESQLHLVVAGTQKSVLMVESEASGLSEEVMLGAVVFGHEQMQVAIKTI
                     NEMVAAVGKPKWDWKPSAISAELDKLVADATTKSLSDAYLITEKQERSAKVKEIKAAA
                     ILALSGGDAPKFTADDVDNSLFKLESNIVRQRILNGEPRIDGRNATTVRPITVKVGVL
                     PRTHGSALFTRGETQALVATTLGTGRDAQIIDALEGERREPFMFHYNFPPFSVGETGM
                     MGSPKRREIGHGNLARRGIAAMMPDMEKFPYVIRIVSEILESNGSSSMASVCGASLSL
                     MDAGVPIKAPVAGVAMGLVLEGERFKVITDILGDEDHLGDMDFKVAGSKDGVTALQMD
                     IKVEGISPEIMKVALAQAKEGRLHILGEMNKVISNTREKMSEWAPSIITLKIDPEKIR
                     DVIGKGGAVIRQITEETGTTIDIENDGTVKIASVSGAAGREALARIELITADVEVGRI
                     YEGKVVRLMDFGAFVTILPGRDGLVHISQISEERVEKVSDKLKEGDNVRVKVLEVDRQ
                     GRVRLSMRDVPASN"
     gene            complement(2573..3016)
                     /locus_tag="VIV63_01780"
     CDS             complement(2573..3016)
                     /locus_tag="VIV63_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY6123353.1"
                     /translation="MSLVAYRAWRERPARGDLPVVPTAEAYSEPADSQGAKKCPKCRS
                     LMSKYRIRSDKNNRLDYCPHCEEIWLDEGEWELVEGLVASGEFTRVFTQAWQYAVRAG
                     ITSAMEEQRLRELLGNDYQRVAGFADWLQTQPGKQEILARLTRRK"
     gene            complement(3191..3697)
                     /gene="phaR"
                     /locus_tag="VIV63_01785"
     CDS             complement(3191..3697)
                     /gene="phaR"
                     /locus_tag="VIV63_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY6123354.1"
                     /translation="MSEPRIIKKYPNRRLYDTVQSKYITLSDIRSLVLERIEFVVIDK
                     KSQEDITRSILLQVIAEQEHGADPVMSRDFLSQVIRSYGGNLQNMLSSYLEQSMKLFG
                     SQQRELRDKVKAVVGMDPVETVAGVAQRNYQRWRSVQDEIFKTLMNATGRSGRDGEDK
                     PSDATSHQ"
     gene            complement(3752..4657)
                     /gene="gluQRS"
                     /locus_tag="VIV63_01790"
     CDS             complement(3752..4657)
                     /gene="gluQRS"
                     /locus_tag="VIV63_01790"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY6123355.1"
                     /translation="MEPLARFAPSPTGPLHLGSLLAAVGSYLDARSVGGRWLVRIEDL
                     DTPRVIPGCADEMLRTLEAFGFEWDGEVLFQSSRRDAYREALGSLAAAGRTYPCSCSR
                     KDLAGLDEEAGYPGTCRHGPARRGPTALRFRVSDTPVHFDDLFLGPQNFDLATCGDVV
                     VQRRDGLTSYQLAVVVDDAFQEITRVVRGADLLASTPWQIDLQHALSLPSPIYGHLPL
                     LLEPDGAKLSKSRRALPLDATAAPSALFSTLTHLSQTPPLELAHSSIKDVWKWAVAHW
                     NPQALAGKGEARCPVQATTKQNPPG"
     gene            complement(4775..5446)
                     /gene="dksA"
                     /locus_tag="VIV63_01795"
     CDS             complement(4775..5446)
                     /gene="dksA"
                     /locus_tag="VIV63_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY6123356.1"
                     /translation="MKAPVAAAKPVPKPVPVPVAAKPAPLASATPVAQAIRKAAPSER
                     IVVTATGVPSSQSGIAASARATAADDGDDNDAGSLLAGPRNVTPYIAKRGEQYMNAVQ
                     LDHFRNILNGWKRDLMEEVDRTVSHMKDEAANFPDPNDRATQEEEFSLELRTRDRERK
                     LIRKIDEALKRVEDGSYGYCLETGEEIGVKRLEARPVATLTIEAQERRERRERQYGDR
                     DDRYR"
     gene            complement(5695..6084)
                     /gene="queF"
                     /locus_tag="VIV63_01800"
     CDS             complement(5695..6084)
                     /gene="queF"
                     /locus_tag="VIV63_01800"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY6123357.1"
                     /translation="MSTQPSTSLETFPNPARATDYTIRMSLPEFTCLCPKTGQPDFAT
                     FELEYVPDELCVELKALKLYMWSFRDRGAFHEAVTNQIADDLAAACAPRFLRLSGKFN
                     VRGGIYTTVVVERRKPGWKPPQVVELP"
     gene            6197..>8485
                     /gene="smc"
                     /locus_tag="VIV63_01805"
     CDS             6197..>8485
                     /gene="smc"
                     /locus_tag="VIV63_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY6123358.1"
                     /translation="MRLTKIKVAGFKSFADPTAVGFPSNLTGVVGPNGCGKSNIIDAV
                     RWVMGELSAKHLRGDSMVDVIFNGSASRKPVGTASVELVFDNSDGKISGPYADYAEVS
                     LRRQVSRDGSSNYFLNGARCRRKDITQLFLGTGLGSRSYAIIEQGMISRVIEAKADDM
                     RAFVEEAAGISRYKERRKETEARIADTRENLERLQDVRDEVDKQIRHLQRQAATARRY
                     QALKETERRLTAELLALRLREIDSGAEIQDSAVRECELRMQQALADQRAAEAAIETQR
                     VYQNEQSERVTAVQGRYYEIGAEISRTEQSITHIRELREQQRNELAQARATLADLNSH
                     IERDEREIASLRAEIEKLSPDLVLAQAAEGTLSESLATTEQEIADWQQRWEGFNREQG
                     AAHQTTQVERARIEQLENQLRRLAGQADRLAVEREALMAQESSALLGQLTEKESMARS
                     SADELSQALSQALDRSQKLRAAQFDAEKNLEHARGDREKRRAEVVSLEALQKAALQRG
                     DSRTAEWLNSSGLGKNPRLAAQLSVNGGWERAVETALGDYLEALCVDELDDVAGVLPS
                     LPVGRVSLLQAGGHMDADGAPGMLSGVVSGPTAIVRLLSRVRLAETLAEALRLRRELA
                     GDESVITRTGEWIGRDWLRVNRGGDAHSDVLEREQRVKQLRVELASSEENTREMEARL
                     ATCREDMATAERERDQAQSRIQGAHREHADLLGQLEGERARSQESTLRRERLEEEAAE
                     VTRELGLGQDTLARARAELDRGL"
BASE COUNT         1579 a         2692 c         2713 g         1501 t
ORIGIN      
        1 ccttttctgt agtattcgcg cccgaaaatc caggtaataa atccgttcgc aaggatcaaa
       61 ttcaagatgt ctctcgccac tgaaaagaaa accggtgtgg ttgcccaatt ccagcgtgca
      121 accaatgaca cagggtctcc cgaagtccag atcgcgctgc tttccgagcg catcaacggc
      181 ctcggcgacc acttcggcgc acacaagcgc gaccatgctt cgcgtcgcgg gctcgtcaag
      241 ctcgtgaacc agcgccgcaa gctcctcgac tacctgaagg ccaccaagcc tgccaagtac
      301 caggaggtcg tcgagcgtct gggcctccgg cgctaactac tcaactccaa aggccgcgca
      361 ttcagcgcgg cctttttcgt ttcatctgtt taaacgcact cgaggcattt gggcctcgtg
      421 aaggaatcaa agcgtgagcg gtctcaagaa atcatttcaa tacggccagc attcggtttc
      481 catcgaaacc ggcgaagtcg cgcgccaggc cagcgcctcc gtcgtcgtct cgatgggtga
      541 caccgtcgtc ctcgtcaccg cggtggccgc caagaaagcg gtcccgggca aggacttctt
      601 cccgctgacc gtcaactaca tcgaaaagac ctacgccgcg ggccgcatcc ccggtggctt
      661 cttcaagcgc gaaggtcgcc ccacggaaaa agagactctg acctcgcgcc tcatcgatcg
      721 cccgatccgt ccgctgttcc ccgacggctt cttcaatgaa gtgcaggtcg tggccatggt
      781 cctgtccatg gacccggata tcgacgccga cattccctcg ctcatcggcg cctccgccgc
      841 gctgtcgctc gcgggcgttc cgttcgccgg ccccatcgcc gccgcacgcg tcggttacaa
      901 gaatggccag taccagctga acccgacgca gaaagagctc aaggaatcgc agctccacct
      961 cgtggtcgcc ggcacgcaga aatccgtgct catggtcgag tccgaagctt cgggcctctc
     1021 tgaagaagtc atgctcggcg ccgtggtgtt cggccacgag cagatgcagg tcgcgatcaa
     1081 gaccatcaac gaaatggttg ccgccgttgg caagccgaag tgggactgga agccgtcggc
     1141 catcagcgcc gagctcgaca agctggtggc ggacgccacc acgaagtcgc tgtccgatgc
     1201 ctatctgatc accgaaaagc aggaacgctc cgccaaggtc aaagagatca aggcagccgc
     1261 gatcctcgcg ctgtcgggtg gcgacgctcc gaagttcacc gccgacgacg tcgacaactc
     1321 actcttcaag ctcgaaagca acatcgtccg ccagcgcatc ctcaatggcg agccgcgcat
     1381 cgacggccgc aatgcgacga cggttcgccc gatcaccgtg aaggtgggcg tgctgccgcg
     1441 tacgcacggt tcggcgctgt tcacgcgtgg tgagacgcag gccctcgtgg cgacgacgct
     1501 cggcaccggc cgcgacgcgc agatcatcga cgcgctcgaa ggcgaacgcc gcgagccgtt
     1561 catgttccac tacaacttcc ctccgttctc cgtcggtgag acgggaatga tgggatcgcc
     1621 gaagcgccgc gaaatcggcc acggcaacct ggcccgccgc ggtatcgccg cgatgatgcc
     1681 ggacatggaa aaattcccgt acgtcatccg catcgtctcg gaaattctcg agtcgaacgg
     1741 ttcctcgtcc atggcctccg tctgcggcgc ttcgctgtcg ctcatggacg cgggcgtgcc
     1801 gatcaaggcg ccggtcgcgg gcgtcgccat gggcctcgtg ctcgaaggcg agcgcttcaa
     1861 ggtcatcacc gacatcctgg gtgacgaaga tcacctgggc gacatggact tcaaggtggc
     1921 cggttcgaaa gacggcgtca ccgcgctgca gatggacatc aaggtcgaag gcatttcgcc
     1981 cgagatcatg aaggtcgcgc tggcccaggc caaagaaggc cgcctgcaca tcctcggcga
     2041 aatgaacaag gtcatctcga acacgcgcga gaagatgtcc gagtgggcgc cttcgatcat
     2101 cacgctgaag atcgacccgg agaagatccg cgacgtcatc ggcaagggtg gcgcggtcat
     2161 ccgccagatc accgaagaga ccggcaccac gatcgacatc gagaacgatg gcacggtcaa
     2221 gatcgccagc gtctcgggcg ccgcgggccg tgaagccctc gcgcgcatcg agttgatcac
     2281 tgccgacgtc gaagtcggcc gtatctacga aggcaaggtc gtccgtctaa tggacttcgg
     2341 cgcgttcgtc accatcctgc cgggccgcga cggtctcgtg cacatctcgc agatcagcga
     2401 ggagcgcgtc gagaaggttt cggacaagct caaggaaggc gacaacgttc gcgtgaaggt
     2461 gctcgaggtc gaccgccagg gtcgcgtgcg cctgtcgatg cgcgacgtgc ctgcgtcgaa
     2521 ctaaaagggg agcattcctc gtttgtggaa acgccgcctt cgggcggcgt tttcatttcc
     2581 ggcgcgtgag cctggccaga atttcctgct tgcccggctg cgtctgcagc cagtcggcaa
     2641 acccggccac gcgctggtaa tcgttgccta acaactcacg caggcgctgt tcttccatgg
     2701 cggaggtgat cccggcgcgc accgcgtatt gccacgcctg cgtgaacacc ctggtgaatt
     2761 cgcccgacgc gaccagtcct tcgacgagtt cccactcgcc ttcatcgagc cagatttcct
     2821 cgcaatgcgg gcaatagtcg aggcgattgt tcttgtcgct gcggatccgg tacttgctca
     2881 tgagcgagcg gcacttcgga cacttcttcg cgccctggga atcggcaggc tcggaatacg
     2941 cttccgcggt cggaacgacg ggcaggtcgc cgcgagccgg cctttcacgc catgcgcggt
     3001 atgcgaccag gctcacgagc gtgccgaggc agtggccgca gctgtatccc gcgaggtcgt
     3061 ccgtcagcag ggtgtgaacg aggaccgaat ggtcacattg cgggcagagc gccatgcagt
     3121 ccggtgggtg agggtacggt aagaggtctc gcgccccgcg gggcgcaaga ccgtgatgat
     3181 tgccgccgaa ctactggtgg ctggtggcgt cggagggctt gtcttcgccg tcacgcccgc
     3241 tacgacccgt agcattcatg agggttttga agatttcgtc ctgcactgac cgccagcgct
     3301 ggtagttgcg ttgtgcgacg ccggcgaccg tctcgaccgg atccatccca acgacagcct
     3361 tcaccttgtc gcgcagttca cgttgctgcg atccgaacag tttcatgctc tgttcgagat
     3421 agctgctcag catgttctgc aggttgccgc cgtacgaacg gatgacctgt gaaagaaagt
     3481 cgcggctcat gaccggatcg gcgccgtgct cctgctcggc gatcacctgc agcaggatgc
     3541 tgcgcgtgat gtcttcctgc gacttcttgt cgatgacgac gaactcgatg cgttcgagca
     3601 cgagcgagcg gatgtcgctg agcgtgatgt acttgctttg caccgtgtcg tagagacgac
     3661 gattcggata cttcttgatg atgcgtggtt cgctcatgcc ttcaccctcc atgtggctgc
     3721 taccttagta cacctcgttg caccccacaa tttatcccgg tggattctgc ttcgttgtcg
     3781 cctgaaccgg acaccgggcc tcgcctttac ccgcaagcgc ttgcgggttc cagtgggcaa
     3841 ctgcccattt ccacacgtct ttaatagaag agtgggccag ttcgaggggt ggggtctgcg
     3901 acaaatgcgt aagggtcgaa aatagtgcgg acggagccgc tgtcgcgtcg agcggaagcg
     3961 cccggcgaga tttcgatagt ttggcgccat ccggctcgag cagcaggggc aggtggccgt
     4021 agataggcga aggcagcgac agtgcgtgct gaagatcgat ctgccatgga gtgctcgcca
     4081 gcagatcggc gccgcgcacc acgcgcgtga tttcctggaa cgcgtcgtcg accaccaccg
     4141 cgagctggta gctagtcagt ccatcccgcc gctgcacgac cacgtcgccg caggtggcga
     4201 ggtcgaaatt ctgcggaccc aggaacagat catcgaagtg gacaggcgta tcgctcacgc
     4261 gaaagcgcaa cgccgtgggt ccgcggcgcg ccggtccgtg ccggcacgtg cctggatatc
     4321 ccgcttcttc gtcgaggccg gcgagatcct tgcgcgaaca gctgcacgga tacgtgcgcc
     4381 cggcggcggc gagtgacccg agcgcttcgc ggtaagcgtc gcggcgcgag ctctggaaca
     4441 gcacctcgcc atcccactcg aaaccgaacg cttcgagagt gcggagcatt tcgtcggcgc
     4501 atcccggaat gactcgcgga gtatcgagat cttctatgcg caccagccag cggccgccca
     4561 cgcttcgcgc gtcgagatag ctgccaacgg ccgccagaag ggaacccagg tgcagaggcc
     4621 cggtgggcga aggcgcgaat cttgccaggg gctccattct caggggctct attctcaggg
     4681 gctccattct caggggctca cgccccacaa cggaaaacgc cgccccgaag gggcggcgtc
     4741 cgtgaacttt caaaagacac ttcgactcgc tcgatcagcg atagcgatcg tcccggtcgc
     4801 cgtactggcg ttcgcggcgc tcgcgacgtt cctgcgcttc gatggtcaac gtggccacgg
     4861 gccgcgcttc gagacgcttc acgccgatct cttcgccggt ctccaggcag tagccgtaag
     4921 aaccgtcttc gacgcgcttc agggcttcgt cgatcttgcg aatgagtttg cgttcgcggt
     4981 cacgcgtgcg cagttcgagg ctgaactctt cttcctgcgt ggcgcgatcg ttcgggtcgg
     5041 ggaaattggc cgcttcgtct ttcatgtgcg agacggtgcg gtcgacttct tccatgaggt
     5101 cgcgcttcca gccattcagg atgttcctga agtggtcgag ctgcacggcg ttcatgtact
     5161 gctcgccacg tttggcgatg tacggcgtta cgtttctcgg accggccagc aggctgccag
     5221 cgtcgttgtc gtcaccatcg tcggccgctg tggcgcgagc gctggcggcg attccggact
     5281 ggctcgaggg cacgcccgtg gcggtgacga cgatacgttc ggacggcgcg gctttgcgga
     5341 ttgcctgggc cacgggtgtg gcgctggcaa gcggcgcggg tttggcagca acaggcacgg
     5401 gtaccggttt cggcacgggc ttggcggctg cgacgggcgc cttcacgggc gcgggttttg
     5461 cggaggtctt ggctgcgacg ggcttagccg ctggcttctt tgcggtcaac ttgggtttct
     5521 cggctttctt aggctttgcg ggctttgcag ccgctcgctt ggtcggcatt gtgggtcgct
     5581 ccaggcccgg atccagaagg gggccggttt gcaggacgcg ggattatacc ggccaacttt
     5641 tgcgtgtaca gcgctctgaa aaagtcggcg gtttggcgat tttttgaagg cagttcaggg
     5701 cagttcgacc acttgcgggg gtttccagcc cggtttacga cgttcgacga ccaccgtcgt
     5761 gtagataccg ccgcgcacat tgaattttcc ggaaagccgc agaaaacgcg gggcgcaggc
     5821 cgcggccagg tcgtcggcaa tctggttggt caccgcttca tggaaagctc cgcggtcgcg
     5881 gaacgaccac atgtagagct tcagcgcctt cagttccacg cacagctcgt ccggcacata
     5941 ttccagttcg aacgtggcga agtcgggctg accagtcttc ggacacagac aggtgaattc
     6001 cggcagcgac atgcggatgg tgtagtccgt cgcgcgcgcg ggatttggaa aagtctcaag
     6061 gctcgtcgaa ggttgcgtgg acatgttaac gaccgggatt tgtcgaaggc cgcgctgttg
     6121 aaacgcggcc cgaaaaaagt tgcagttact ttacactacg ccgccaattc atccgcacgc
     6181 gagacgcgag taaacgatgc gcctgaccaa gatcaaagtc gccggattca aatcgttcgc
     6241 ggacccgacc gcggtcggct tcccgagcaa tctcaccggc gtcgtcggtc ctaacggctg
     6301 cggcaaatcg aacatcatcg acgccgtgcg ctgggtgatg ggcgagctgt cggcgaaaca
     6361 tctgcgcggc gactcgatgg tcgacgtcat cttcaacggc tctgcctcgc gcaagcccgt
     6421 gggcacggcg tcggtggagc tggtgttcga caacagcgac ggcaagatct ccgggccgta
     6481 cgccgattac gcggaggtgt cgctgcgacg ccaggtctcg cgcgacggct cgtctaacta
     6541 tttcctcaac ggcgcgcgtt gccgccgcaa ggacatcacg cagctgttcc tcggcaccgg
     6601 tctcggcagc cgcagctacg ccatcatcga gcagggcatg atctcgcgcg tcatcgaggc
     6661 caaggccgat gacatgcgcg cgttcgtcga agaggccgcc ggcatttcgc gctacaagga
     6721 gcgccgcaag gaaaccgagg cgcgtatcgc cgacacgcgc gaaaacctcg agcgccttca
     6781 ggacgtgcgc gacgaagtcg acaagcagat ccgccacctg cagcgccagg ctgcgaccgc
     6841 gcgccgttac caggccctca aggaaaccga gcgccgcctg accgccgaac tgctggcgct
     6901 gcgactgcgc gaaatcgatt ccggcgccga aattcaggac agcgccgtgc gcgaatgcga
     6961 actgcgcatg cagcaggcgc tcgccgatca gcgcgccgcc gaagccgcca tcgaaacgca
     7021 gcgcgtgtat cagaacgagc agtccgagcg cgtcaccgcc gtgcagggac gctattacga
     7081 gatcggcgcg gagatcagcc gcaccgaaca gtcgatcacc cacatccgcg agctgcgcga
     7141 acagcagcgc aatgaactcg cgcaggcgcg cgccacgctg gccgacctga actcgcacat
     7201 cgaacgcgac gaacgcgaaa tcgccagtct gcgcgccgag atcgaaaagc tatcgcccga
     7261 cctcgtgttg gcccaggccg cggaaggcac gctcagcgag agcctggcga ccaccgagca
     7321 ggagatcgcc gactggcagc agcgctggga aggattcaat cgcgagcagg gcgccgctca
     7381 ccagaccacg caggtggaac gcgcgcgcat cgagcagctg gaaaaccagt tgcggcggct
     7441 cgccggccag gccgatcgcc tggcggtcga acgcgaagcc ctcatggcgc aggaatcttc
     7501 ggcgctgctc gggcagctga ccgaaaagga atcgatggcg cgaagctccg cggacgagtt
     7561 gtcgcaggcg ctcagccagg cgctcgaccg ctcgcagaaa ctgcgcgccg cgcagttcga
     7621 tgccgagaag aatctcgaac atgcgcgcgg cgatcgcgaa aagcgtcgcg ccgaagtggt
     7681 ctcgctggaa gcgctgcaga aggcggcgct gcaacggggc gactcgcgaa cggccgagtg
     7741 gctcaactcg tcgggcctag gcaagaatcc gcgtcttgcg gcgcagctgt cggtgaacgg
     7801 cggctgggag cgcgcggtcg aaacggcgct cggcgattac ctcgaagctt tatgtgtcga
     7861 cgaactcgac gacgttgccg gtgttttgcc gtcgctgccc gtgggccgcg tctcgctgct
     7921 gcaggcaggc ggccacatgg atgccgacgg cgctcccggc atgttgtcgg gcgtggtcag
     7981 cggacccacc gccatcgtgc ggctgctgtc gcgggtgcgg ctggccgaaa cgctcgcgga
     8041 agcgctgcgc ctgcgccgcg agctcgccgg cgacgaatcc gtcatcacgc gcacgggcga
     8101 gtggataggc cgcgactggc tgcgcgtcaa tcgtggtgga gatgcgcatt ccgacgtgct
     8161 cgagcgcgaa cagcgcgtca agcagctgcg cgtcgagctc gcgtccagcg aagagaacac
     8221 gcgtgaaatg gaagcgcgtc tcgccacctg tcgcgaagac atggcgaccg cggagcgcga
     8281 gcgcgaccag gcacagagcc gcatccaggg cgcccaccgc gagcacgccg acctgctcgg
     8341 ccagctcgaa ggcgagcggg cccgttcaca ggaatccacg ctgcgccgcg agcgcctgga
     8401 agaagaggcc gcggaagtca cgcgcgagct cggtctcggc caggacacgc tggcgcgggc
     8461 acgtgccgaa ctcgatcgcg gcctg
//
LOCUS       DATDEU010000077         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_888996, whole genome shotgun sequence.
ACCESSION   DATDEU010000077 DATDEU010000000
VERSION     DATDEU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888996"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VIV63_01810"
     CDS             <1..796
                     /locus_tag="VIV63_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123359.1"
                     /translation="QVSTYLSWQHGEKAGISQFVNGISNVVEAEKTEAYEIGLKTVLF
                     NDTLVFNTAVFYSEIEDYQQSVRVVDEYTTNLNLAAGIPPGQATAYTSATGNVPLVKV
                     KGIEIDGVYAGIPNTSIRFSGAYNDAYYAEFPNLAQPVENGYPGASPYRDASGETLPG
                     SFKYAFNIGVDYRAPFSEALEWHATANAAWQDKFNSDVALSSYAWIPDRAIVDLSIGW
                     GTRSGSFDASLLLKNAFDDNTHVSQTWNSYGPAFPRWWGVVFSGKL"
     gene            921..1202
                     /locus_tag="VIV63_01815"
     CDS             921..1202
                     /locus_tag="VIV63_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123360.1"
                     /translation="MSFLVRRAIPDYRSVKGNIDVAILQRHERDVTHFLTVTHWQSEE
                     AIRAFAGDDLLKAKYYPEDEQFLLEFEPEVQHFVVAAVESADSPERQRT"
     gene            1269..2201
                     /gene="dmeF"
                     /locus_tag="VIV63_01820"
     CDS             1269..2201
                     /gene="dmeF"
                     /locus_tag="VIV63_01820"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEY6123361.1"
                     /translation="MNDSDPHHCHTTRFDSGNPLAETNIRRALWLTAIMMVVEIAGGW
                     WFNSMAVLADGWHMSSHALALGLSAFAYSFARRQANNRRYAFGTWKVEVLGGYTSAIF
                     LLGIAALMTFQSIERLLRPQAIHYGEAIAIAIVGLAVNLLCAWWLRGSHSHDDAPGHT
                     HGHDHSHHEDMNMRSAYLHVLADAATSVLAIVALFGGLMWGAAWLDPVMGLAGAVLVS
                     VWAIGLLRDSGRILLDAEMDSPVVAEIREVIEQGVVPARITDLHVWRVGRAQYAVVVG
                     VTTPSNENSEYFRQALRVHEELAHITVEVERVPG"
     gene            complement(2227..3204)
                     /locus_tag="VIV63_01825"
     CDS             complement(2227..3204)
                     /locus_tag="VIV63_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6123362.1"
                     /translation="MKLIQLRYLVAVAENDLNITQAARVLHAAQPGVSKQLKQLEDEL
                     GFQLFERKGRALVRPTDAGTKVIDRASQIVRQARSIKALASELRKDEAGSLSIATTHT
                     QARYVLPQVLKRFRDKYPKVRLHLHQGNSEQIADLATTDRIDLAIATGASPMFSGLVR
                     LPCFRWHRAIVVPDGHPLGEISKPTMAELAQYPLVTYSFSFSGPASLVELFAEEGLTP
                     NVALTAWDSDVIKKYVREGLGVGILANIAIDGEEDADLKVRDASHLFPVHTTWAGFRR
                     GVLLRGYIYDFLQMLAPHLTQRQVQAAENAANQEEVDREFLAVKIPFLR"
BASE COUNT          610 a         1033 c         1114 g          640 t
ORIGIN      
        1 gcaggtgtct acctacctgt cctggcagca cggcgagaag gcgggtatct cgcagttcgt
       61 gaacggcatt tcgaacgtgg tcgaagccga gaagaccgag gcgtacgaga tcggtctcaa
      121 gaccgtgctg ttcaacgaca cactggtgtt caacacggcc gtgttctatt cggagatcga
      181 ggactatcag cagtccgtgc gcgtagtcga tgagtacacg accaacctga acctcgcggc
      241 gggtattccg ccgggccagg cgacggctta caccagcgcc acgggcaacg tgccgctcgt
      301 caaggtgaag ggcatcgaga tcgacggcgt ctacgccggc atcccgaaca cgtcgattcg
      361 tttctcgggc gcctacaacg acgcctacta cgcggagttc ccgaatctcg cgcagccggt
      421 cgagaacggc tatccgggcg cgtcgccgta tcgcgatgcg agcggcgaga ctctgccggg
      481 ctcgttcaag tacgcgttca acatcggcgt ggactaccgc gcaccgttca gcgaggcgct
      541 ggaatggcac gcaacggcca acgccgcctg gcaggacaag ttcaacagcg acgtcgcgtt
      601 gtccagttac gcatggattc ccgatcgtgc gatcgtggac ctgtcgattg gctggggcac
      661 tcgcagcggc agcttcgacg cgagcctgct gctgaagaac gccttcgacg acaacaccca
      721 cgtgtcgcag acgtggaaca gctacggtcc cgcgttcccg cgctggtggg gagtggtgtt
      781 cagcggcaag ttgtaattaa gtctggctga actgaggtgt agaacttcct gtcgatgccg
      841 gcggcgggcc gctgcagtat gcgcgcatga tttgtcgcat ctggcatggt cgaactccgc
      901 gttccaaggc gcacgcatac gtgtcgttcc tggtacggcg cgcgatcccc gactaccggt
      961 cggtgaaagg gaacatcgat gtggcgatcc tgcagcggca cgagcgcgac gtaacgcatt
     1021 tcctgaccgt cacacactgg cagtcggaag aggcgattcg tgcattcgcc ggtgacgatc
     1081 tgctgaaggc aaagtattac ccggaagacg agcagttcct gctggaattc gaaccggaag
     1141 ttcagcactt cgtcgtcgcg gcagtcgaga gcgccgacag tccggagcgt cagcgcacgt
     1201 aggtgcgcga atgacaccgt gcgccgcgcc tcgcacgcgg caatccgcgt gagttaccct
     1261 cccggggtat gaacgactcg gacccgcatc actgccatac gacccgtttc gattccggaa
     1321 acccgctggc cgagaccaat atccggcgcg cgttgtggct gacggccatc atgatggtcg
     1381 tcgagatcgc cggcggctgg tggttcaatt cgatggcggt gctggcggac ggctggcaca
     1441 tgagctctca cgcgctggcg ctcgggctct cggcgttcgc ctattccttc gcgaggaggc
     1501 aggcgaataa ccggcgctat gcgttcggca cgtggaaggt ggaagttctc ggcggctaca
     1561 ccagcgcgat cttcctgctc ggcatcgccg cgctcatgac gtttcagtcg atcgagcggt
     1621 tgctgcggcc gcaggccatc cactatggag aagccatcgc gatcgcgatc gtggggctcg
     1681 cggtgaacct gctttgtgcc tggtggttgc gaggctcgca ttcgcacgac gacgcgcccg
     1741 ggcacacgca tgggcacgat cattcccatc acgaagacat gaacatgcgc tcggcctacc
     1801 tgcacgtgct cgccgacgcc gcgacttccg tgctcgccat cgtcgcgctg ttcggcggcc
     1861 tgatgtgggg cgccgcgtgg ctggatccgg tcatgggact tgcaggtgcg gtgttggttt
     1921 cggtctgggc gattgggctg ttgcgagact cggggcgaat cctgctcgac gccgagatgg
     1981 actcgcccgt cgtcgccgag atacgcgaag tcatcgagca gggcgtggtg cccgcgcgca
     2041 tcaccgatct gcatgtgtgg cgcgtcggcc gtgcccagta cgcggtggtg gtgggagtca
     2101 ccacaccttc aaacgagaac agcgaatatt tccggcaggc gctgcgcgtt cacgaggagc
     2161 ttgcgcacat caccgttgaa gtcgagcgag tcccgggttg aggccgacac gtcttcgcct
     2221 ccgcggtcag cgcaggaacg gaatcttcac cgccaggaat tcgcgatcca cttcctcctg
     2281 gttcgcggcg ttttccgcgg cctgcacctg acgctgcgtg aggtgcggcg cgagcatctg
     2341 caggaaatcg tagatgtagc cgcgcagcag cacgccgcgc cggaatccgg cccacgtggt
     2401 gtgcaccggg aacaggtgcg aggcgtcgcg caccttgaga tccgcgtcct cttcgccgtc
     2461 gatggcgatg ttggcgagga tgccgacgcc caatccctca cgaacatatt tcttgatgac
     2521 gtccgagtcc cacgcggtga gcgccacatt cggagtcagg ccttcttcgg caaagagctc
     2581 cacgagcgac gcgggtccgc tgaaactgaa tgaataggtg acgagcgggt actgcgcgag
     2641 ctccgccatc gtgggtttac tgatctcgcc gagcggatgg ccatcgggaa cgacgatggc
     2701 gcgatgccag cgaaagcagg gcaggcgcac gaggccggaa aacatcgggc tcgcacccgt
     2761 ggcgatggcg agatcgatgc ggtcggtggt cgcgaggtcg gcgatctgtt cggaattgcc
     2821 ctggtggagg tgcagccgca ccttcggata tttgtcgcgg aatcgcttga gcacctgcgg
     2881 caacacgtaa cgcgcctgcg tgtgcgtggt ggcaatcgac agcgagccgg cttcgtcctt
     2941 gcgcaactcc gaggcaagcg ccttgatgct gcgtgcctgg cgcacgatct gcgaggcgcg
     3001 gtcgatgacc ttggtgccgg cgtccgtagg ccgcaccagc gcgcggccct tgcgttcgaa
     3061 caactgaaag ccgagttcgt cctcgagctg cttgagctgt ttactcacgc cgggctgcgc
     3121 cgcgtgcaac acgcgcgcgg cctgcgtgat gttgaggtcg ttctccgcta ccgccacgag
     3181 gtagcgtaat tggatcaatt tcatctggat tggtgggcca tcggaggcgg tgagggtgaa
     3241 aacttcgaga gggggactct tttctcagct cactcacagc ccccgcggcc cgtggaaaga
     3301 gtacggcgct cgaggcgccg cacccactaa ctaatgagca tcaacagatt cgattccggc
     3361 atctcactgc gtgtagatct ggtcgaacac gccgccg
//
LOCUS       DATDEU010000078         5765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1180772, whole genome shotgun sequence.
ACCESSION   DATDEU010000078 DATDEU010000000
VERSION     DATDEU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180772"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2471)
                     /locus_tag="VIV63_01830"
     CDS             complement(<1..2471)
                     /locus_tag="VIV63_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY6123363.1"
                     /translation="MRIGRLLAVFVLLLAGSVPSVAQVPTAEQLELLRTMSPEDREAL
                     MEQLGVGGSMGDSSSATGASGRRRDGREDEQNDQNRQFNAQDFSELQRDPKFKPNDSL
                     LVEIDFIKDKPPRIESQGQGLPPITIPGELAPVLEPLEAERLEELITLIRSRNPYVLD
                     SNGAMLLPGFAPVTLAGLTEEKATLRLKAMPAFRKLEVKAVLLTVRKAGVSALQPFGY
                     DLFKESSSTFAPVTDVPVPSDYVVGPGDQLNVQLFGSQNRNLRLVVGRDGRVSFPEIG
                     PISVAGRTFERVAADLEERVSRQIIGVRASVSMGDTRSIRVFVMGEANRPGSYTVSGL
                     AAMSSALYAAGGIKPIGSLRDIQLKRAGAVVRRFDLYDLLLRGDTSDDTKLQPGDVIF
                     IPPISATVAVDGEVHRPAIYEIKGETTVADMIALAGGLTSEADTGRVALVRVNDQRNR
                     VVVDVPLDVASGRTERLRNGDSLRVLRLRPTLDRGVTIEGHVFRPASVAWREGMRLTD
                     LLGSVDELRPNADINYVLVRRELPPDRRTVFVSADLAAALRDPSSPKNITLTPRDRVI
                     VFDSESGREQLLSPLLEEVRRQSRFDNPSEVVRIDGRVKARGDYPLEPRMRVSDLLRA
                     GGGLQDAAYGAKAELTRYRVGGESRQTQLLEVDLAAVLRGDPDADLLLQPFDFLNVKE
                     VPEWSEQEQVQLMGEVRFPGLYPIQRGETLNSVLLRAGGLTGLAFPDGTVFTRKELVE
                     REQQQLERLAERLQSDLASVALQATAANQGGASQALTVGQSLLNQLKNTKAVGRLVID
                     LQSVIDAPRGSQQDIVLREGDRIVVP"
     gene            complement(2471..3940)
                     /locus_tag="VIV63_01835"
     CDS             complement(2471..3940)
                     /locus_tag="VIV63_01835"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEY6123364.1"
                     /translation="MSQSLRRLIVVIFLAGCLTNEAQADAWLPPGDEGLRHDIELLAD
                     AGILRGPVTTWPLSWPDIARDALAASFSDFTGATADALLRVQRIARVASDTGFTGAGI
                     RLSAAHEPLMLRGFADTPREEGEVAMRAGWLGDHVALNLQGEYVIDPVDGKQWRADGS
                     YLGVNIGNFMISAGFMERWWGPGWDGSLILSTNARPIPSLTLERNYTDPFKTKWLSWI
                     GPWRASIAVGEAESRGVAMPDVRFLAMRVNFKPRPWMEFGLTRTAQACGGDRECDLTT
                     LGNLLIGRDNQVEGGAASSEQPGNQMAGYDLRLRSPWRPLPLVFYTQWIGEDEAGGFP
                     SRFLGLFGLETWGDSPLGGFRLRAEYADTACNFPSDQPYLDCAYRNGIYPQGYTYRGR
                     VIGHSMDNDSRMYSFGGILARPGGDVLSVTVRRVEINRNGGFHAISEAPLDLDNVELR
                     YSRTFDIGKFSVGAGYDHRSQAAESDSEARGFVTWQQGF"
     gene            complement(3937..5436)
                     /locus_tag="VIV63_01840"
     CDS             complement(3937..5436)
                     /locus_tag="VIV63_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEY6123365.1"
                     /translation="MRSRHPARAGWLPIGSLIALLVAPSAFADGGWFESGDSVLRADL
                     QLLNDAEVIRLPINQWPIPRAAVRYALDNARDHFAGNTAVAAALARVRERAGAINTFS
                     YDASLTAGQAGLLRDFDTVGREDAELRGGLRYTADRGEVALNVTAAVNPSDGEEWRLD
                     GSHATVQWGNWLFSANTLDRWWGASHESSLILSNNARPMPTLLIERATAQPFKNVLLS
                     WLGPWRMSFGISRMEEERLDIDAPLFMAWRVVVMPFKDIELGFSRTAQFCGEQLSCDL
                     ETFGNMLIGNDNVGIDSTAETEPGNQMAGFDIRWASPIGNWPYAIYSQFIGEDESSYT
                     PAKFLSQYGLEAWKPLVDGGMVLTYAEFATTTCSASSGRGPYFNCAYNQGRFNVEGYR
                     YEGRSIGHTTDGDAESYALGASYVTPGGEYWSAVVRAAHLNRDPIPDARNTVSGVPAE
                     YGAVEVGWRGRAFGAPISVQLGVESLQPDGGERNVDAFGFVSWRYEFKP"
     gene            complement(5433..>5765)
                     /locus_tag="VIV63_01845"
     CDS             complement(5433..>5765)
                     /locus_tag="VIV63_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123366.1"
                     /translation="TVTELTARKVAPERIELLARHKVHAGNRPVSIVAFKRLDARTLG
                     SLVALYEHKVFTQSVVWGINAFDQWGVELGKKMCEEVSPLVRDPDGAVGTRAMLRPLL
                     GQLSKWRS"
BASE COUNT          989 a         1864 c         1938 g          974 t
ORIGIN      
        1 ggcacgacga tgcgatcgcc ttcgcgcagc acgatgtcct gctgcgaacc gcgcggcgcg
       61 tcgatgaccg attgcagatc gatgacgaga cggcccaccg ccttcgtgtt cttgagctgg
      121 ttgagcagtg actggcccac cgtcagcgcc tggctcgcgc cgccctggtt cgcggccgtg
      181 gcctgcaggg ccacggaagc gagatcgctc tgcaggcgct cggcgagccg ttcgagctgc
      241 tgttgttcgc gctcgacgag ttccttgcgc gtgaagaccg tgccgtccgg gaaggcgagc
      301 ccggtgagcc cgccggcgcg cagcagcacc gagttcaagg tctcgccgcg ctggatcgga
      361 tacaggccgg ggaagcgcac ttcacccatc aactggacct gctcctgctc gctccactcc
      421 ggcacttcct tgacgttcag gaaatcgaag ggctgcagca ggaggtcggc gtccgggtcg
      481 ccgcgaagca cggccgccag gtcgacttcg agtagctggg tctggcgcga ttcgccgccg
      541 acgcgataac gcgtgagctc ggccttggcg ccatatgcgg cgtcctgcag tccgccaccg
      601 gcccgcagca ggtcgctgac acgcatgcgc ggttcgagtg gatagtcgcc gcgcgccttg
      661 acgcggccgt cgatgcgcac gacctcgctg ggattgtcga aacgggactg gcggcggact
      721 tcttcgagca gcggcgaaag cagctgctcc cggcccgatt cggagtcgaa cacgatgacg
      781 cgatcgcgtg gcgtcagcgt gatgttcttg ggcgaggacg gatcgcgcaa cgcggccgcg
      841 agatcggcgg agacgaagac ggtgcggcgg tcgggcggca attcgcggcg caccagcacg
      901 tagttgatgt cggcgttggg gcgcaattcg tcgaccgagc ccagaaggtc ggtgagccgc
      961 atgccttcgc gccaggcgac gctggccggg cggaacacat ggccttcgat cgtcacaccg
     1021 cgatcgaggg tgggccggag ccgcagcact cgcaatgagt cgccgttgcg cagacgttcg
     1081 gtgcggccgg acgcgacgtc gagcggcacg tcgaccacga cacgattgcg ctgatcgttg
     1141 acccgcacca gcgcgacgcg cccggtgtcg gcttcgctcg tgaggccacc cgccagcgcg
     1201 atcatgtccg cgacggtggt ctcgcccttg atctcgtaga tggccgggcg atggacttca
     1261 ccatcgactg ccaccgttgc ggagatcggc ggaatgaaga tgacgtcgcc gggctgtagt
     1321 ttggtgtcgt cgctggtatc gccgcgtaac aacagatcgt agagatcgaa gcggcgcacg
     1381 accgcgccgg cgcgtttgag ctggatgtcg cgcagcgagc cgatgggctt gatgccgccg
     1441 gcggcgtaca gcgcggagct catggccgcg agtccgctga ccgtgtagct gcccgggcga
     1501 ttcgcctcgc ccatgacgaa cacgcgaatg gaccgcgtat cgcccatgct cacgctggcg
     1561 cgcacgccga tgatctggcg cgacacgcgc tcttcgaggt cggcggccac gcgttcgaaa
     1621 gtccggcccg cgacactgat gggcccgatc tcggggaagc tcacgcggcc atcgcgtccg
     1681 accaccagtc gcaggttgcg gttctggctg ccgaacaact gcacgttgag ctggtcgccg
     1741 ggacctacga cgtagtcgga cgggacgggt acgtcggtca ccggcgcgaa cgtcgatgag
     1801 ctttccttga agaggtcgta accaaaaggc tgcagtgccg agacgcccgc cttgcgcacg
     1861 gtcagcagca cggccttcac ttcgagcttc cggaatgccg gcatcgcctt cagtcgaagc
     1921 gtggccttct cttcagtgag gcccgccagc gtgaccggcg cgaagcccgg cagcagcatg
     1981 gcgccgttcg agtcgagcac gtagggattg cgcgagcgga tgagagtgat gagttcctcg
     2041 agccgctccg cttcgagcgg ctcgagcacc ggcgccagct cgccggggat ggtgatgggc
     2101 ggcagcccct gcccctggct ttcaatgcgc ggcggcttgt ccttgatgaa atcgatttcg
     2161 acgagcaggg agtcgttcgg cttgaacttg gggtcccgct gcagctcgct gaaatcctgc
     2221 gcgttgaact ggcggttctg atcgttctgt tcgtcttcac gcccgtcccg gcggcggccg
     2281 gacgcgcccg tggccgacga gctgtcgccc atcgagccgc cgacgccgag ctgctccatg
     2341 agcgcttcgc gatcttcggg gctcatggtg cgaaggagtt cgagctgctc ggcggtggga
     2401 acctgtgcca cggacggcac gctgccggcc agcaacagga caaacaccgc caacaatcgt
     2461 ccgatacgca tcaaaatccc tgttgccaag tgacgaaacc acgagcttct gaatctgatt
     2521 ccgcagcctg gctgcggtgg tcgtacccgg cgccgacgct gaattttcca atgtcgaacg
     2581 tccgcgagta gcgcaattct acattgtcga gatcgagggg cgcttcgctg attgcatgga
     2641 agccgccgtt gcggttgatc tcgacccggc gcacggtgac gctgagcacg tcaccgcccg
     2701 ggcgcgcgag gatgccgccg aacgagtaca tgcggctgtc gttgtccatc gaatgcccga
     2761 tgacccggcc gcggtatgtg tagccctgcg ggtagatgcc attgcggtag gcgcagtcga
     2821 ggtaaggctg gtcgctcggg aagttgcacg ccgtgtccgc atattcggcg cgcaggcgga
     2881 aaccgccgag ggggctgtcg ccccaggtct cgagaccaaa caatcccagg aaacgcgacg
     2941 gaaacccgcc cgcttcgtcc tcacctatcc actgggtgta gaacacgagc ggcagcggac
     3001 gccacggcga gcgcagacgc aggtcgtaac cggccatctg gttgccgggc tgctccgacg
     3061 atgcggcgcc cccttcgacc tggttgtcac ggccgatcag cagattcccg agcgtggtga
     3121 ggtcgcattc gcgatcgccg ccgcaggcct gcgcggtgcg cgtcagaccg aactccatcc
     3181 acggccgcgg cttgaaattc acgcgcatgg cgaggaaccg gacatcgggc atggcgacgc
     3241 cgcgtgactc cgcttcgccc accgcgatgc tcgcgcgcca gggtccgatc cacgacaacc
     3301 atttggtctt gaacggatcg gtgtaattgc gctcgagcgt caggctcgga atgggccgcg
     3361 cattggtcga caggatcagg ctgccatccc agcccggccc ccaccagcgc tccatgaacc
     3421 ccgcggagat catgaaattg ccgatgttca cgccaaggta ggaaccgtcg gcgcgccatt
     3481 gtttgccgtc gaccggatcg atgacgtact cgccctggag gttgagcgcg acgtgatctc
     3541 ccagccagcc ggcgcgcatc gcgacttcgc cttcttcgcg cggcgtgtcc gcgaagccgc
     3601 gcagcatcaa aggttcgtgc gcggccgaca ggcggatgcc cgcgcccgtg aagcccgtgt
     3661 ccgaggcgac gcgtgcgatg cgctgaacgc gcagcaacgc atccgcggtc gcgccggtga
     3721 aatcgctgaa actggcggcg agcgcatcgc gcgcgatatc gggccacgac agcggccagg
     3781 tggtgaccgg cccgcgcagg atgccggcat ccgcgagcag ctcgatgtcg tggcgcagcc
     3841 cttcatcgcc cggcggcagc caggcatccg cctgtgcctc gttagtcagg cacccggcga
     3901 gaaagatgac gacgatgaga cgacggagcg actgactcac ggtttgaatt cgtaacgcca
     3961 gctcacgaat ccaaaggcgt cgacgttgcg ctcgccaccg tcgggttgaa gggattcgac
     4021 gcccagttgc acggagatcg gcgcgccgaa cgcgcggccg cgccagccga cttcgaccgc
     4081 gccgtattcg gccggaacgc cgctcacggt attgcgcgcg tcggggatgg gatcgcgatt
     4141 gagatgcgcg gcgcgcacga cggccgacca gtattcgccg cccggtgtca cgtagctcgc
     4201 gccgagcgcg taactttcgg catcaccatc ggtggtgtgg ccgatcgagc gtccctcgta
     4261 tcgataaccc tcgacgttga accggccctg gttgtacgcg caattgaagt aaggcccgcg
     4321 gccactcgac gccgaacacg tcgtggtcgc gaattctgcg taggtcagca ccatgccgcc
     4381 gtcgaccagg ggtttccagg cctcgaggcc gtattgcgac aggaacttcg ccggcgtgta
     4441 gctcgattca tcttcgccga tgaattgcga gtagatagcg tagggccagt tgccgatggg
     4501 tgatgcccag cggatatcga agccggccat ctggttgccg ggctcggtct cggccgtgct
     4561 gtcgatgccg acgttgtcgt tgccgatcag catgttgccg aaagtctcga ggtcgcaact
     4621 cagctgctcg ccgcagaact gcgcggtgcg cgaaaaaccg agttcgatgt ccttgaacgg
     4681 catgaccacg acgcgccagg ccatgaacag cggcgcgtcg atgtcgaggc gctcctcttc
     4741 catgcggctg atgccgaagc tcatgcgcca gggtccgagc cagctcagca gcacgttctt
     4801 gaaaggctgc gcggtggcgc gttcgatgag cagcgtgggc atcggccgcg cgttgttgga
     4861 gaggatgaga ctgctttcgt gggatgcgcc ccaccagcgg tcgagcgtgt tggcgctgaa
     4921 cagccagttg ccccactgga ccgtggcgtg tgaaccgtcg agacgccatt cctcgccatc
     4981 ggacggattt acggccgcag tgacgttgag tgcaacctca ccgcgatcgg ccgtgtagcg
     5041 cagaccgccg cgcagctcgg cgtcttcgcg accgacggtg tcgaagtccc ggagcaagcc
     5101 ggcctgcccc gcggtgagcg aggcgtcgta cgaaaaggta ttgatggcgc cggcacgctc
     5161 acgcacgcgg gcgagcgcgg ccgcgacggc ggtgttaccg gcgaaatgat cgcgcgcgtt
     5221 gtcgagcgcg taacgcaccg cggcgcgcgg aatcggccac tggttgatgg gcaggcgaat
     5281 gacttccgca tcgttcagca gctgaaggtc ggcgcgcagc acggaatcgc cggattcgaa
     5341 ccagccgcca tcggcaaatg cggagggcgc gaccagcagc gctatcaacg acccaatggg
     5401 cagccatccg gcgcgagccg ggtgccgcga tctcaagaac gccacttcga caattgtccc
     5461 agcaacgggc gcagcatggc gcgcgtgccg accgcgccgt cggggtcgcg caccagcggc
     5521 gacacttctt cacacatttt cttgcccaac tcgacacccc actgatcgaa cgcgttgatc
     5581 ccccagacca cgctctgagt gaacactttg tgttcgtaga gtgcgaccaa actaccgaga
     5641 gtccgggcat ccagtcgctt gaaagcgaca atgctcacgg ggcggttccc ggcatggacc
     5701 ttgtgacggg cgagaagttc gatgcgctcg ggcgcaacct tgcgcgctgt gagttcggtc
     5761 acggt
//
LOCUS       DATDEU010000079         2295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1472950, whole genome shotgun sequence.
ACCESSION   DATDEU010000079 DATDEU010000000
VERSION     DATDEU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472950"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1241)
                     /locus_tag="VIV63_01850"
     CDS             complement(222..1241)
                     /locus_tag="VIV63_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123367.1"
                     /translation="MPPVPRGLFYVSDQTRGIHRVRLRARKKAGRKDSPPRFQYVGPT
                     GRRIREARTLARIARLAIPPAYEDVWICASPRGHLQATGRDARGRKQYRYHADWTRAR
                     GDHKHSRMRKFGKGLGRLRAGVRRDLKRPGLPREKVLAIVVKLLDATQVRVGNEEYAR
                     SNGSFGLTTLRDRHARFPRGKLLLQFRGKGGAPHDVLVDDAQVAKLVRRCQELPGQAL
                     FQYVDEQGGLRAVDSGQVNAYLRERMGEEFTAKDFRTWHATLHALTLLAKVPVPEKRS
                     ERALRLRINAVVKEVAAGLRNTPAVCRKSYINPAVFEAWQSGALRRRPVRSTPAKSLL
                     TLLRK"
     gene            complement(1352..>2295)
                     /locus_tag="VIV63_01855"
     CDS             complement(1352..>2295)
                     /locus_tag="VIV63_01855"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY6123368.1"
                     /translation="PRVQLKGWATEESMRRLVKAGGQDLDKLIAAAKSRNFKPIPLSL
                     TTSIKFDNKVSRVRTANVAGLLPGSDPALKSEVVIYSAHHDHFGIGEPDKTGDKIYNG
                     AEDNASGCAQLLAIARAMAALPERPRRSVLMLFVAGEEQGLLGSKYYSLHPTFAPGKI
                     AANINYDGGNYRGRAKDITYLGHGKSSLDRIVDALAARQGRVVVPDQFPDKGYFYRSD
                     QFNFAKIGAPAMYFGRSTDYIGKPAGWGRTQEEDWTEHVYHQPSDEISSEWVFDGMIE
                     DAAIGFYAGWLIAQADAMPTWNPGDEFEAARKKALAQ"
BASE COUNT          385 a          770 c          725 g          415 t
ORIGIN      
        1 tgcacaagaa cgcgcccata ccgaagctgc cggagaacct cgcacggcat cagcaattcc
       61 tcgagcagct ggtctcgaag aagccggacg agcgtttttc gagcgccgcc aacgctttca
      121 ccgcgctcat ggagaattcg atgagcgaag ccgcgagcac cgccgccgcg taaatggaga
      181 aatggggaca ctccccattt cctagcaaaa ggggccgcac ctcatttgcg aagtaacgtg
      241 agcagcgatt tcgccggcgt tgagcgaaca ggacgccggc gcaacgctcc gctctgccag
      301 gcctcgaaca ccgcggggtt gatgtacgac ttgcgacaaa ccgcgggcgt attgcgcagg
      361 cccgccgcca cttccttgac caccgcattg atgcgcaatc gcagcgcacg ctcactgcgc
      421 ttttcgggca ccggcacctt cgcgagcagg gtcagcgcat gcagcgtcgc gtgccacgtg
      481 cggaaatcct tggcggtgaa ttcctctccc atgcgctcgc gcaggtaagc gttgacctgc
      541 cccgaatcga cggcgcgcag cccgccctgc tcgtccacgt actggaacag cgcctggccc
      601 ggcaattcct gacagcggcg cacgagcttt gccacctgcg cgtcatcgac cagcacgtcg
      661 tgcggagcgc cgcccttgcc gcggaactgc agcagcagtt tgccacgcgg gaaacgcgcg
      721 tgacggtcgc gcaaggtagt caggccgaag ctgccgttgc tgcgcgcata ttcttcattg
      781 cccacgcgca cctgtgtcgc atccagcagt ttcaccacta tcgcaagcac cttctcgcgc
      841 ggcagaccgg gacgtttgag atcgcggcgc acgcccgcgc gcagccgtcc gaggcccttg
      901 ccgaatttcc gcatgcgcga atgcttgtga tcgccgcgcg cccgcgtcca gtccgcgtga
      961 tatcgatact gcttgcgccc ccgcgcatca cgacccgtgg cctgcagatg accgcgtggg
     1021 ctcgcgcaga tccagacatc ctcatacgcc ggcggtatgg caagcctcgc gatacgcgcc
     1081 agcgtgcgag cctcgcgaat gcgccgtccc gtcgggccga cgtactggaa tcgcggtggc
     1141 gagtccttcc ggcccgcttt cttccgggcg cgaagccgca cgcgatgaat tccgcgtgtc
     1201 tgatcactga cgtagaagag acctcgggga accggcggca tgcggcaagc ctgcaacccg
     1261 cgccgcggtg ccgatgtagg tatcgctgaa cttgaacggc atgcccgacg cgattccgtg
     1321 tcaggcttcg ccgttcacct tgtacacgcg cctactgcgc cagcgccttc ttgcgcgcgg
     1381 cctcgaactc gtcgccggga ttccacgtcg gcatggcatc cgcctgggcg atcagccacc
     1441 cggcataaaa gccgatcgcc gcgtcttcga tcatgccgtc gaacacccac tcgctggaga
     1501 tctcgtcact cggctggtga tagacgtgtt cggtccagtc ttcttcctgc gtgcggcccc
     1561 agccggcggg cttgccgatg tagtcggtgc tgcggccgaa atacatggcc ggcgcgccga
     1621 tcttggcgaa gttgaactgg tcggagcggt agaaataacc cttgtcgggg aactggtcgg
     1681 gcaccacgac gcggccctgt ctggcggcca gcgcatcgac gatgcggtcg agcgaggact
     1741 ttccgtgccc gaggtatgtg atgtccttcg cgcggccgcg atagttgccg ccgtcgtagt
     1801 tgatgttggc ggcgatcttg ccgggcgcga acgtgggatg cagcgagtag tacttgctgc
     1861 ctaacaaccc ctgttcctcg ccggcgacga acaacatgag cacggagcgc cgcggccgct
     1921 cgggcagcgc cgccattgcg cgcgcgatgg ccagaagctg cgcgcagccc gacgcattgt
     1981 cttcggcgcc gttgtagatc ttgtcgccgg tcttgtccgg ctcgccgatg ccgaagtggt
     2041 cgtggtgcgc ggagtagatc accacttcgc tcttgagagc cgggtcgctg ccgggcaaca
     2101 gcccggccac gtttgcggtg cgcacgcgcg acaccttgtt gtcgaacttg atggaggtgg
     2161 tgagcgaaag cggaatgggc ttgaagttgc ggctcttcgc cgcggcgatg agcttgtcga
     2221 gatcctggcc gcccgccttc acgagacgcc gcatggactc ttccgtggcc cagcccttga
     2281 gctggacgcg cggct
//
LOCUS       DATDEU010000080         4886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_160602, whole genome shotgun sequence.
ACCESSION   DATDEU010000080 DATDEU010000000
VERSION     DATDEU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160602"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="VIV63_01860"
     CDS             complement(<1..1343)
                     /locus_tag="VIV63_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6123369.1"
                     /translation="MSAAKVTVDVAGILKRLGVDSINAGAWMGNLGWSKDTAGSAITS
                     TNPATGEVLAQVRSATVTDYENVMRCAVEAAAAWRNVPAPRRGEAIRLLGDALRAAKE
                     DLGALVTLENGKIKAEGLGEVQEMIDIADFALGQSRMLYGLTMHSERPQHRMYEQWHP
                     LGVVGIISAFNFPVAVWAWNAFLAAICGNASVWKPSPKTPLTAIAVQHICNKVMRDHK
                     LPAIFQLFIDGGTELATRFVDDRRVALVSFTGSTAIGRNVAERVARRLGKSLLELGGN
                     NAIIVDETADLKLAVPAIVFGAVGTAGQRCTTTRRVLVHRSRIDELGKRLTHAYTQVR
                     IGNPIDPATLMGPLIDKGAIERYSQAIAQAKAAGGKVITGEKVLEGKGNFVAPTIIIA
                     ENDWPIVQHETFAPVMYLIPFDSLEEALEMQNASAHGLSSAIFTDRLQHAEAFLSA"
     gene            complement(1340..2545)
                     /locus_tag="VIV63_01865"
     CDS             complement(1340..2545)
                     /locus_tag="VIV63_01865"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6123370.1"
                     /translation="MHKVLILGAGKIGALISGLLSESGDYDVTLGDVDASAAEAVVKA
                     HGSGNLRAVALDASDPKALENHFSVNKPQAVISSLPYYCNPAVAKAARKGNAHYFDLT
                     EDVAVTQAVRRISRGASKAFVPQCGLAPGFISIAANELTKHFDELRSIKLRVGALPQH
                     PNNVLKYSLTWSTEGLINEYGNPCQSVVDGKVVDAAPLEGLEEIEIDGTLYEAFNTSG
                     GLGSLAESHGKKCEQINYKTMRYPGHCAQMRLLMNDLKLNSDRATLKRVLEHAVPQTL
                     QDVVIVYAAVAGKQDGELREENYVNKIYPQMIAGRLWSAIQVTTAAGITSVVDLVMSN
                     PRKYQGFVAQEQFDLPEILDNRFGQYYAPGGTKDISSVVVVSGQAGHQRKAAAKAAAK
                     APAKPRKKK"
     gene            2603..3958
                     /locus_tag="VIV63_01870"
     CDS             2603..3958
                     /locus_tag="VIV63_01870"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY6123371.1"
                     /translation="MRTPDTASWHPASWTSRTAAQQPRYNDLAALETAVASLSRLPPL
                     VVSWEIEQLRERLAEAQIGRKFLLMGGDCAETFEECESDKIAKKLKILLQMSLVLLHG
                     LKKPIIRVGRMAGQYAKPRSADTETRNGVTLPAFRGDLVNRSEFTAAAREPDPTLLLR
                     GYERAALTLNFVRALCDGGFADLHHPRYWDLGFMKHSPLIEQYQKIVDSIGDSLDFFE
                     RVSGGRAPHEVTAVDFFAAHEGLLMPYEQAQTRYIERQKRWYNLGTHMPWIGMRTAAL
                     DGAHVEFFRGIANPMGVKIGPAMTAEWLQGLVTTLNPQNTPGRLTIIHRFGAKDVEKG
                     LPAMIKAVRETGQQVLWVCDPMHGNTETSSGGLKTRRFENILKELELSFQVHEANGSF
                     LGGVHIELTGEDVTECTGGARGLTDADLARAYKSTVDPRLNYEQALEMAMLIGRHGPA
                     R"
     gene            3976..>4886
                     /locus_tag="VIV63_01875"
     CDS             3976..>4886
                     /locus_tag="VIV63_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY6123372.1"
                     /translation="MQYERITVPKDGQRITINPDASLNVPDNPLIAFIEGDGIGIDIT
                     PVMLKVVNAAVAKAYGGKRKISWVEVFAGDKAAVLYGTGFPDETLTALRDFVVSIKGP
                     LGTPVGGGMRSLNVAMRQNLDLYACVRPIRYFPGVSTPMADASLTDMVVFRENTEDIY
                     AGIEWPTGSNEVHKLIHFLQTELKATGILFPASSGIGIKPVSKEGSQRLVRKAIQYAI
                     ENDRVSVTLVHKGNIMKYTEGAFMQWGYLLAKEEYGAQPIGAGPWLTFKNPINGKDIV
                     IKDVIADNFLQQVLLRPEDYDVIATLNL"
BASE COUNT          878 a         1560 c         1538 g          910 t
ORIGIN      
        1 gcggacagga acgcctccgc gtgttgcagc cggtcggtga agatcgccga cgacaacccg
       61 tgcgccgatg cgttctgcat ctcgagcgct tcttcgagcg agtcgaacgg gatgaggtac
      121 atcaccggcg cgaaagtttc gtgctgcacg atgggccagt cgttctccgc gatgatgatg
      181 gtcggcgcga cgaagttgcc cttgccctcg agcaccttct cgcccgtgat caccttgccg
      241 cccgcggcct tggcctgggc gatcgcctgc gaataccgtt cgatcgcgcc cttgtcgatg
      301 agcgggccca tgagtgtggc cgggtcgatc ggattgccga tgcgcacctg cgtgtacgcg
      361 tgggtgaggc gtttgccgag ctcgtcgatg cgcgagcgat gcacgaggac tcttcgcgtc
      421 gtcgtgcagc gttgacccgc agtgccgacc gcgccgaata cgatcgcggg cacggcgagt
      481 ttcaggtccg cggtctcgtc gacgatgatg gcgttgttgc cgccgagttc gagcagcgac
      541 ttgccgagcc ggcgtgcgac acgctcggcg acattgcggc cgatggccgt ggagccggtg
      601 aacgacacca gtgccacgcg ccggtcgtcg acgaatcggg tggcgagttc cgtgccgccg
      661 tcgatgaaca gctggaagat cgcgggtagt ttgtggtcgc gcatcacctt gttgcagatg
      721 tgctgaaccg cgatcgccgt gagcggcgtc ttgggcgagg gcttccacac cgaggcgttg
      781 ccgcagatgg cggcgaggaa cgcgttccac gcccacacgg ctaccgggaa attgaacgcc
      841 gagatgattc cgaccacgcc gagcggatgc cactgctcgt acatgcggtg ctgcggacgc
      901 tcggaatgca tggtgaggcc atagagcatg cgcgactggc cgagagcgaa gtcggcgata
      961 tcgatcatct cctggacttc acccaggcct tcggccttga tcttgccgtt ctcgagcgtg
     1021 accagcgcac ccaggtcttc cttggcggca cgcaacgcat cgcctaacaa ccggatggct
     1081 tcgccccgtc gcggcgcggg gacgttgcgc cacgcggccg cggcttccac cgcgcagcgc
     1141 atgacgttct cgtagtcggt gacggtcgcg ctgcgcacct gcgccagcac ttcgcccgtg
     1201 gccggattgg tcgacgtgat ggcgctgccg gccgtgtcct tcgaccagcc gagattgccc
     1261 atccacgcgc cggcgttgat ggagtcgacg ccgagccgct tgagaatgcc ggcgacgtcc
     1321 accgttacct tcgcggcgct cacttctttt tcctcggctt ggcgggagcc ttcgccgccg
     1381 ccttcgcggc tgccttgcgc tgatgtccgg cctggccgct gaccacgacg accgaggaaa
     1441 tgtccttggt gccgcccggc gcgtagtact ggccgaaacg gttgtcgaga atttcgggca
     1501 ggtcgaactg ttcctgcgcg acgaagccct ggtacttgcg cgggttcgac atgacgaggt
     1561 cgacgaccga cgtgatgccg gctgccgtgg tcacctggat ggccgaccag agacggcccg
     1621 cgatcatctg cggatagatc ttgttgacgt agttttcctc acgaagctcg ccgtcctgtt
     1681 tgccggcgac ggctgcgtac acgatgacca cgtcctgcaa cgtctgcggc acggcgtgtt
     1741 cgagcacacg cttcaacgtg gcgcgatccg agttgagctt gaggtcgttc atgagcaggc
     1801 gcatctgcgc gcagtgcccg ggataccgca tggtcttgta gttgatctgt tcgcacttct
     1861 ttccatgcga ctcggccagc gaccccaggc cacccgacgt gttgaaggct tcgtacaacg
     1921 tgccgtcgat ttcgatttcc tcgagccctt cgagcggcgc ggcgtcgacg accttgccgt
     1981 cgaccaccga ctggcaggga ttgccgtatt cgttgatgag tccttcggtg gaccaggtca
     2041 gcgaatactt gagcacgttg ttagggtgct ggggcagggc gccgacgcgc agcttgatgg
     2101 agcgcaactc atcgaagtgc ttcgtgagtt cgttggcggc gatgctgatg aagcccggcg
     2161 cgagtccaca ttggggcacg aacgccttcg atgcaccacg cgagatgcgt cgcacggcct
     2221 gcgtgaccgc cacgtcctcg gtgagatcga aataatgggc gttgcccttg cgcgcggcct
     2281 tggccacggc cggattgcag tagtagggca ggctggagat gaccgcctgg ggtttgttca
     2341 ccgagaagtg gttttcgagt gccttggggt cgcttgcgtc aagggcaacc gccctcaagt
     2401 ttcccgaacc gtgagctttc accacggctt cggcggccga cgcgtcgacg tcacccagcg
     2461 tgacgtcata atcaccggac tcggataaca aaccagagat gagggcgccg atcttgccgg
     2521 ctcccaggat taaaactttg tgcatgaata cctcgattga cggtagtgtg ccgtaacctg
     2581 ctgaattaga atacttccca ccatgcgtac ccccgacact gcctcctggc atccagctag
     2641 ctggacctcc cgcaccgccg ctcaacagcc gcgttacaac gacctcgccg cgctcgagac
     2701 cgcggtcgca tcgctctcgc gtttgccgcc gctggtggtg tcctgggaaa tcgaacagct
     2761 gcgagagcgg ctcgccgaag cgcagatcgg tcgcaagttc ctgctcatgg gtggcgactg
     2821 cgccgagacg ttcgaagagt gcgaatccga caagatcgcc aagaagctca agatccttct
     2881 gcagatgagc ctcgtgctgc tgcacggact caagaaaccc atcattcgcg tcggccgcat
     2941 ggccggccaa tacgccaagc cgcgttcggc cgataccgaa acccgcaatg gcgtcacgct
     3001 gccggcgttc cgcggcgatc tcgtgaatcg ttccgaattc accgccgccg cgcgcgagcc
     3061 ggatcccacg ttgctgctgc gcggctatga acgcgccgcg ctcacgttga acttcgtgcg
     3121 cgcgttgtgt gacggcggct tcgccgacct gcatcacccg agatattggg atctcggatt
     3181 catgaagcat tcgccgttga tcgagcagta ccagaagatc gtcgactcca tcggcgactc
     3241 cctggacttc ttcgaacgcg tgagcggtgg ccgtgcgccg catgaagtga ccgcggtcga
     3301 cttcttcgcc gcgcacgaag gtctgctcat gccctacgag caggcacaga cgcgttacat
     3361 cgaacgccag aagcgctggt acaacctcgg cacgcacatg ccgtggatcg gcatgcgtac
     3421 ggccgcgctc gatggcgcgc acgtggaatt cttccgcggc attgccaacc ccatgggcgt
     3481 gaagatcggg cccgccatga cggctgaatg gctgcaaggt ctcgtcacca cgctgaatcc
     3541 gcagaacacg ccgggccgcc tcaccatcat tcaccgcttc ggcgcgaagg atgtggagaa
     3601 gggcctgccc gcgatgatca aggccgttcg cgagaccggt cagcaagtgt tgtgggtttg
     3661 cgatcccatg cacggaaata cggagaccag ctccggcggc ctcaagacgc gccgtttcga
     3721 gaacatcctc aaggagctcg aactgtcatt ccaggttcac gaagccaatg gctcgttcct
     3781 ggggggcgtg cacatcgagc ttacgggcga agacgtcacg gaatgcacgg gtggagcccg
     3841 cggcctcacc gatgccgacc tcgcgcgcgc gtacaagtcg acggttgacc ctcgcctgaa
     3901 ctacgaacag gcgctcgaga tggcgatgct catcggacgt cacggcccgg cacggtaatt
     3961 gctaccctct ccgctatgca gtacgaacgc ataacggttc ccaaagacgg ccagcgcatc
     4021 accatcaatc cggacgcgtc gctgaacgtg ccggataacc cgctcatcgc cttcatagag
     4081 ggcgacggga tcggcatcga catcacgccg gtgatgctca aggtggtcaa cgccgcggtc
     4141 gcgaaggcct acggcggcaa gcgcaagatc tcctgggtag aggtcttcgc cggcgacaag
     4201 gcggcggtgc tctacggcac ggggttcccc gacgagacgc tcaccgcgct gcgcgacttc
     4261 gtcgtgtcca tcaagggacc gctcggcacg cctgtcggcg gcggcatgcg ttcgctcaac
     4321 gtggcgatgc gccagaacct cgacctctac gcctgcgtgc ggcccattcg ctatttcccc
     4381 ggcgtctcga cgccgatggc ggatgcctcg ctcaccgaca tggtggtgtt ccgcgagaac
     4441 accgaagaca tctacgccgg catcgaatgg cccacgggct cgaacgaggt ccacaagctc
     4501 attcacttcc tgcagaccga gctcaaggca acgggcattc ttttccccgc gagctccggc
     4561 atcggcatca agcccgtgtc gaaggaaggc agccagcgcc tggtgcgcaa ggccatccag
     4621 tacgccatcg agaacgaccg cgtatcggtc acgctggtgc acaagggcaa catcatgaag
     4681 tacaccgaag gtgccttcat gcagtggggc tatctactgg cgaaggaaga gtacggcgcg
     4741 cagcccatcg gcgcgggacc gtggctcacg ttcaagaacc cgatcaacgg caaggacatc
     4801 gtcatcaagg acgtgatcgc cgacaacttc ctgcagcagg tactgctgcg tccggaagat
     4861 tacgacgtga tcgcgacgct gaacct
//
LOCUS       DATDEU010000081         8018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1619695, whole genome shotgun sequence.
ACCESSION   DATDEU010000081 DATDEU010000000
VERSION     DATDEU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619695"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1622
                     /locus_tag="VIV63_01880"
     CDS             282..1622
                     /locus_tag="VIV63_01880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6123373.1"
                     /translation="MNQNWWFTTLLVNEHRYCPPLTRDIKCDVVIVGAGFAGVSAAIE
                     FLKTGHKVVLIDKNIVGGSSSGRSAGFLTPDSELELHQLVRRYGVKAAGEIWAAPLSG
                     IERLVASIQKYDVQCGLQKQDSLFLGLGKGGKEAVDSELHCRQEVGFTDQRTYDEQQL
                     QAILGSVGYTAGIRYGGTYGVNPLACLQGFKNLLVDHGMEIYESTEMERLEDHTVYTH
                     GGSITADKIIIAVDKLDESISPLSHEVFHAQTFLSITEPLTDKELRILFPSGEQMQCW
                     DSKLVYTYFRLTADKRLLLGGGTPITTYLKDAFNSPRIIRKIIKDFTDHFPGLGDLSF
                     MQFWPGQIDATRDLLPTIAKPPGMPHVRFIFGAVGIPWAAFTGSFAARNVLGAADADY
                     EKYFNYFSNDRKFALPSSLGKIIGKPALFALTNSWAKFYQVDKLREAGQLEKEF"
     gene            1559..3061
                     /locus_tag="VIV63_01885"
     CDS             1559..3061
                     /locus_tag="VIV63_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6123374.1"
                     /translation="MGEVLPGRQAARGRSAGERILRPAFTKCPGAAATLLACLLAAGC
                     TVGPDYERPEIEAPEKYRFAVVEANDLANTPWWQQFHDPTLDELIQVALSDNFDVRIA
                     AARVDEFYSSLGITRSGKFPQIGAEGAVSTGRTPPASAADSVRVDAFASWEIDLFGRL
                     RRLTEASRADLLASEEGRRFAVLSLVSTVASTYITLLAVDAQLDIAKRTLVSREKALR
                     IFEARYRRGATSEFELSQSRSEYAVTKSTIPPLEQAQVQIENALALLLGRNPGPITRD
                     RTLAALGMPIVPSGLPSEILERRPDVKQAELNLIAANARIGAAKALYYPSISLTGLFG
                     SVSNSFDTLFTGPAELYSYGASVAGPIFTGGGISGQVGVAEARQQQSLLAYQRAIRTA
                     FRDVENALIAGQKSREALAAQEERVAAMRDYARLANLRYDNGYSGYLEVLDAERGLFS
                     AELDYTQAKSDTYTALVNLYTTMGGGWVVDAAALASQPSVDMSQDPKSFP"
     gene            3071..4207
                     /locus_tag="VIV63_01890"
     CDS             3071..4207
                     /locus_tag="VIV63_01890"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY6123375.1"
                     /translation="MDTQVNTPTAETETPAATKPKDPVKTITKAVLITLIVLFVYHVA
                     SDRVTPYTSQSNIEVLLVQIAPQVSGQVIEVATRDNERVKKGQLLYRIDPEPFEISLR
                     AAEANLAMVQQNLKVSTAEVRSTQAALSKQRTQLATSKELGGIIFDLTKEKALSETDS
                     IRARSEIDRSTADLARAEAELESAKQKLGPAGDANPQLQQARAALAQARLDFTNTTVL
                     APADGMITNLRLSKGQYANRGQPVMSFIEGNSVWLTAYLRENQLSKVAPGNAVRVTFD
                     LLPGRVFDGNVDSVGWGVTNGGDEAPVGNLPDVQSQQGWLRDPQRFPVRIALKEEASN
                     ALGDLPLRNGAQANVLILTNPNSWLLNPIGRAWLRIVSWFSYIY"
     gene            4210..5232
                     /locus_tag="VIV63_01895"
     CDS             4210..5232
                     /locus_tag="VIV63_01895"
                     /inference="COORDINATES: protein motif:HMM:NF022616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HEY6123376.1"
                     /translation="MDSAHVHAVLRFAFAVTLAFVVAELMEWTPPFLSPVLTCVVMAN
                     IPMRPPLKVAIGFSLVIGASAFFALTLSYALRGVPYILFGLSTLIVFHSLYALAKGAS
                     KLGPLFIIICVTAIPVVGMQSLAAAQHFAEAFAAGACFAIFMAWVSWLLFPKPAPLRA
                     APEATALSPDIALRSALLGTAILAPLMLAYLMLGLHTALPVMIGTVMIVATLDFHLGR
                     KQAALRVIANFCGGVSSVVVLILLAIHPTFATFVLLVLASALLFGLRISHGDPMAQLL
                     TVACNGYLIVFGTALHSDSGTFDVWLTRVGYFFLAGVFTIGMMALIWPPTLKPDPTPA
                     QTGDGP"
     gene            5229..5357
                     /locus_tag="VIV63_01900"
     CDS             5229..5357
                     /locus_tag="VIV63_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123377.1"
                     /translation="MKAIAPPTGRLWTGVGVFVVGWIITLSLVPFVTRSDLPMSAG"
     gene            complement(5363..5749)
                     /locus_tag="VIV63_01905"
     CDS             complement(5363..5749)
                     /locus_tag="VIV63_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DVU3141 family protein"
                     /protein_id="HEY6123378.1"
                     /translation="MRVACAALLAFTACMGPASFGQANLGELRDAPSAKFTPQDFEML
                     WAAVDEVSRGAKTGVVKKWANAETGNGGAIKLLNVFTSTDGRDCRRLRMDNYAKTLKG
                     SSKQIVCADPEGKWMMDADARPAPKP"
     gene            complement(5746..6342)
                     /locus_tag="VIV63_01910"
     CDS             complement(5746..6342)
                     /locus_tag="VIV63_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY6123379.1"
                     /translation="MTLPRLRGLLAASLLLCVSAALTGCGTAKVKVRTRVDPAAEFSG
                     FRSYSFVPVLGTNYGGKTTALTVYFMQAIRHEMDSRGYRYTDESPDLLVNFNANPRAT
                     GESTTSISPSYGYSTGYYAYRAELYGLPVIAFEDEETVSYRVGTANIDVVDARRKMLA
                     WEGLAEGRLTEDMLNNPGPAIAAIVSDIFAQYPAKAAP"
     gene            6481..7158
                     /locus_tag="VIV63_01915"
     CDS             6481..7158
                     /locus_tag="VIV63_01915"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HEY6123380.1"
                     /translation="MNKSITRAITLSCLFAVVFASVGMAKEKPPENWDGLERKKVKGL
                     DNVYVRPNVEFTPYKTVMLDPTQVEFSKHWEKSQDFRNRPTAEDMQKIKDKLAELMHS
                     VFTEELVENGYTVVTTANEDTLQVRTAIIDLFINAPDLQDPGITRSYTTSAGAMTLVL
                     EARDGPTGQLLARAVDRQEDDHMGGYMMWTNAATNTADARRIIQVWAKKLRESLDRLN
                     GKEPKKK"
     gene            complement(7171..>8018)
                     /locus_tag="VIV63_01920"
     CDS             complement(7171..>8018)
                     /locus_tag="VIV63_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY6123381.1"
                     /translation="GAQELEPRAYSPSPVGLNFVLFAGSRSTGEILPDPSVPIENIEA
                     TIDAGVAGYGRTFALWGQSASVAVAVPYVWGDVSGDVGEERRAVTRDGLGDVRLRLAT
                     NLLGGPALTPKEFMQRTPRSTLGASLVVIAPTGQYYSDKLINIGTNRWAFKPELGFSM
                     PHNRWMFDVYAGLWLFTDNDEFFGGVRRSQDPITTIQGHVSYTIRPRLWIALDSTYYF
                     GGQSTVDGVLKADRKENSRLGVTFSLPIGARQSIKASWSEGASTRTGNDFSTWGVAWQ
                     YAWFD"
BASE COUNT         1514 a         2598 c         2432 g         1474 t
ORIGIN      
        1 cagagtcgta cgcgtcaggt ctggcgatgt tccaacattc atccggcgcc cctgcaattt
       61 attggtcgat ttgataccgg atggctccac ttcgatcatg ggaccaaggt ctcattcccg
      121 ttggaggtcc gcagttcgat attgctgccg catcgcggcg aactatctat gcgcacgccc
      181 tcatgcatgg ccaattggca gcggcaccag actggctcgc atcccgctaa caaaatcaga
      241 cgaaaaggtg caggtgcaat ttggccaaga aattccggct ggtgaatcag aattggtggt
      301 tcacgacgct tctcgtcaac gaacatcgtt actgccctcc gctcacgcgc gacatcaagt
      361 gcgacgtggt gatcgtcggc gcgggattcg ccggcgtctc tgcggccatc gaattcctga
      421 agaccggcca caaagtggtt ctgatcgaca agaacatcgt cggcggcagc tcatccggac
      481 gcagcgcggg attcctgacg cccgacagcg agctcgagct gcaccagctg gtgcgtcgct
      541 atggtgtcaa ggcggccggc gagatctggg ctgcaccgct ctccggcatc gaacgactcg
      601 tggcgtccat tcagaaatac gacgtccaat gcggcctcca gaagcaggac tccctgtttc
      661 tgggcctggg caaaggcggc aaggaggccg tggactcgga gctgcattgc cgccaggaag
      721 tcggcttcac ggaccagcga acctacgatg agcaacagct ccaggcgatc ctgggatcgg
      781 tgggttatac cgccggcatt cgctatggcg gcacctatgg cgtcaatccg ctggcatgcc
      841 tgcaaggttt caagaatctt ctggtcgacc atggcatgga gatctacgag agcacggaga
      901 tggaacgcct cgaagatcac accgtgtata cgcatggagg cagcatcacg gccgacaaga
      961 tcatcatcgc ggtggacaag ctcgatgagt ccataagccc gttatcgcat gaagtgtttc
     1021 acgcgcagac gttcctgagc atcaccgagc cgctcacgga caaggagctg cgcatcctct
     1081 tcccgtccgg tgaacagatg cagtgctggg attccaagct cgtgtacacg tactttcgac
     1141 tgacagccga caaacgtctc ctgctcggcg gcggcacgcc catcaccacc tatctcaagg
     1201 atgcgttcaa cagtccgcgc atcatccgca agatcatcaa ggacttcacc gatcacttcc
     1261 ccgggttggg cgacctgtcg ttcatgcagt tctggccggg gcagatcgat gccacgcgcg
     1321 acctgctgcc caccatcgcc aagccgccgg gaatgccgca cgtacgattc atcttcggtg
     1381 ccgtgggcat tccgtgggcg gcgttcaccg gcagtttcgc ggcgcgcaac gtcctcggtg
     1441 cggcggatgc ggactacgag aagtacttca attatttttc gaacgaccga aagttcgcgc
     1501 tgcccagcag cctcggcaag atcattggca agccggcatt gttcgcgctg accaatagtt
     1561 gggcgaagtt ctaccaggtc gacaagctgc gcgaggccgg tcagctggag aaagaattct
     1621 gaggccggcg ttcaccaagt gtcccggcgc ggcggcaacc ctgctcgcct gcctgcttgc
     1681 ggccggatgc accgtcggcc ccgattacga aaggccggag atcgaagctc ccgaaaaata
     1741 ccgctttgcc gtggtcgaag ccaatgacct cgccaacacg ccgtggtggc agcagttcca
     1801 cgatccgacg ctcgacgagc tcatccaggt cgcactctcg gacaacttcg atgtacgaat
     1861 cgcggcggcc cgcgtcgacg agttctacag ttcgctcggg atcacccgtt cgggaaagtt
     1921 tccgcaaatc ggcgcggaag gcgccgtcag caccggacgc acgccaccgg ccagtgccgc
     1981 ggatagtgtc cgcgtcgatg cgttcgcttc gtgggagata gacctcttcg ggcggctgcg
     2041 ccgcctcacc gaggcgagcc gcgcggattt gctggccagc gaagaaggcc ggcgcttcgc
     2101 ggttctctcg ctggtgtcga cggtggcatc gacttacatc acgctgctgg ccgtcgacgc
     2161 gcagctcgac atcgccaaac gcacgctggt cagccgcgag aaggcgctga ggatcttcga
     2221 agcacgctac cggcgcggtg cgacgtcgga gttcgaattg tcccagtccc ggtcggaata
     2281 cgccgtcacc aagtcgacga ttccacctct cgaacaggcg caggtgcaga tcgaaaacgc
     2341 gctggcgttg ctgctcggga ggaatccggg accgatcacg cgcgacagga cgctcgcggc
     2401 acttggaatg cccatcgtgc cgtcgggtct tccttccgaa attctcgagc gccggccgga
     2461 cgtcaagcag gccgaactca acctcatcgc agccaatgcg cgcatcggcg cggcgaaggc
     2521 gctgtactac ccgagcatct cgctgaccgg gttgttcggc agcgtgagca attcattcga
     2581 cacgttattc accgggcccg ccgagctcta cagctatggc gcgtcggtcg ccggtcccat
     2641 cttcacgggc ggcggcattt ccggacaggt tggcgtcgcc gaggcgcgcc agcagcaatc
     2701 gctgctcgcc tatcagcgcg cgatccgtac cgcgtttcgc gatgtcgaaa atgcattgat
     2761 tgccggccag aaatcgcgcg aagcgctcgc ggcacaggaa gaacgtgtgg ccgcgatgcg
     2821 tgattacgca aggctcgcca acctgcgcta cgacaatggt tactcgggtt atctcgaggt
     2881 gctcgatgcg gaacgtggcc tcttctccgc ggaactcgac tacacgcagg ccaagagcga
     2941 cacctacacc gcactggtga atctctatac gacgatgggc ggcggctggg tagtcgatgc
     3001 agctgcgctg gcttcccagc ccagcgtcga catgtcgcag gatccaaaat ctttcccctg
     3061 aagaagctca atggacacac aggtcaacac tccaaccgcc gagaccgaga cgccggcggc
     3121 cacgaagccg aaagatccgg tgaagacgat cacgaaggcg gtcctcatca cgttgatcgt
     3181 gctgttcgtc tatcacgtgg catcggatcg cgtcacaccc tacacctcgc agtcgaatat
     3241 cgaggtgctg ctcgtacaga tcgcgccaca ggtgtccggg caggtgatcg aagtcgccac
     3301 gcgcgacaac gaacgcgtga agaagggaca acttctgtat cgaatcgacc cggagccatt
     3361 cgagatctcg ttgcgggccg cggaagccaa cctcgcgatg gtccagcaga acctcaaggt
     3421 gtcgaccgcc gaggtgcgtt ccacccaggc ggcgctcagc aagcagcgca cgcaactcgc
     3481 gacgagcaag gagttgggcg gcatcatctt cgacctgacc aaggaaaagg cgctctcgga
     3541 aacggattcc atccgcgcgc gctcggagat cgaccgctcg accgcggacc ttgcacgtgc
     3601 cgaggccgaa ctggaaagcg cgaaacagaa actcggtccc gcgggtgatg ccaatcccca
     3661 gttgcagcag gcgcgcgcgg cgctcgcgca ggcgcgcctc gatttcacca atacgacggt
     3721 gctcgcgccc gccgacggca tgatcacgaa ccttcgcctg tcgaagggac agtacgccaa
     3781 tcgcggccag ccggtgatga gcttcatcga aggcaacagc gtctggctga ctgcctatct
     3841 acgcgagaac cagctcagca aggtggcgcc cggcaatgcc gttcgcgtca ccttcgacct
     3901 gttgccgggc cgcgtattcg atggcaatgt cgacagcgtt ggctggggcg tcaccaatgg
     3961 cggcgacgaa gcgcccgtcg gcaatcttcc cgacgtccag tcgcaacagg gatggctgcg
     4021 cgatccgcag cgcttcccgg tgcgcatcgc gctcaaggaa gaagccagca atgcgctcgg
     4081 cgacctgcca ctgcgcaatg gcgcgcaggc caacgtgctg atcctcacca accccaattc
     4141 ctggctgctg aacccgatcg gccgcgcatg gctgcgcatc gtctcctggt tcagctacat
     4201 ctactgacga tggattccgc acacgtccat gccgtgttgc gcttcgcgtt cgccgtaaca
     4261 ctggcattcg ttgtcgcgga actcatggaa tggacgccgc cgtttctctc gcccgtgctg
     4321 acctgcgtgg tgatggccaa catccccatg cgtccgccgc tcaaagtcgc catcggcttc
     4381 agcctcgtca tcggcgcgtc ggcgttcttc gcgctcacgc tctcctacgc actgcgcggc
     4441 gtgccataca tcctgttcgg actgagcact ctcatcgtgt tccactcgct gtatgcgctc
     4501 gcgaagggag catcgaagct cggcccgctg ttcatcatca tctgcgtgac agccatcccc
     4561 gttgtaggca tgcaatcgct ggcggccgcg cagcatttcg ccgaggcatt tgccgccggg
     4621 gcctgcttcg ccatcttcat ggcctgggtg agctggctgc tgtttcccaa gcccgcgccg
     4681 cttcgcgcag ctccggaagc caccgcgttg tcgcctgaca tcgcgctccg cagtgcgctc
     4741 ctcggaacgg ccatcctcgc cccgctcatg ctcgcctacc tgatgctggg cctgcacacc
     4801 gcgctgcccg tcatgatcgg cactgtgatg atcgtcgcca cgctggattt ccatctcggg
     4861 cgcaaacagg cggcgctacg cgtgatcgcc aacttttgcg ggggcgtctc cagcgtggtg
     4921 gtgctgatcc tgctcgccat ccacccgacc ttcgcgacct tcgtgctgct ggtgctggcc
     4981 tcggcgctgc tgttcggtct gcggatttct cacggcgatc cgatggccca gctgctgacc
     5041 gtggcgtgca acggttacct catcgttttc ggcaccgcgc tgcactctga cagcggaaca
     5101 ttcgacgtgt ggcttacgcg cgtcggctat ttcttcctgg ccggcgtctt cacgatcggg
     5161 atgatggcgc tgatctggcc acccactctc aaacccgacc ccactcccgc acaaactgga
     5221 gacggcccat gaaggcaatt gcccctccca cgggacgact gtggactggc gtcggtgtct
     5281 tcgtggtcgg ctggatcatc acgctgtcgc tcgtaccttt cgtgacccgc tcggacctgc
     5341 cgatgtccgc cggttagacc aatcagggct tgggcgcggg acgcgcgtcc gcatccatca
     5401 tccacttgcc ttcggggtcg gcgcacacga tctgcttcga tgaacccttg agcgtcttcg
     5461 cgtagttgtc catgcgcagg cggcggcaat cacggccatc ggtgctggtg aacacgttca
     5521 gtagtttgat cgcgccgcca ttgcccgttt cggcgttcgc ccacttcttg acgacaccgg
     5581 tcttcgcacc gcggctcact tcgtcgacgg ccgcccagag catctcgaaa tcctgcggcg
     5641 tgaacttggc gctgggagcg tcacgcagtt cgccgagatt cgcctgaccg aacgacgccg
     5701 ggcccatgca cgcggtgaaa gccagcagcg ccgcacacgc gactctcatg gcgccgcctt
     5761 ggccggatac tgcgcgaaga tgtcggagac gatcgcggcg atcgcgggcc ccggattgtt
     5821 cagcatgtct tctgtgagcc ttccttcggc gagcccttcc cacgccagca tcttgcggcg
     5881 ggcgtcgacg acgtcgatgt tcgcggtgcc gacgcggtag ctcaccgtct cctcgtcctc
     5941 gaacgcgatc acgggcaatc catagagttc cgcgcggtac gcgtagtagc cggtcgaata
     6001 gccatacgac ggcgaaatgc tggtggtcga ctcgccggtg gcgcgcggat tggcattgaa
     6061 gttcacgagc aggtccggcg attcgtcggt gtaccggtaa ccgcgtgaat ccatctcgtg
     6121 ccggatcgcc tgcatgaaat agacggtgag cgccgtcgtc ttgccgccgt agttcgtgcc
     6181 gagcaccggg acgaagctgt agctgcggaa acccgagaac tcggccgcgg ggtcgacacg
     6241 cgtgcgtacc ttgacctttg cggtgccaca accggtgagc gccgcgctga cgcaaagcaa
     6301 caacgaagcg gcgagcaggc ccctgaggcg cggcaatgtc atggcgggtt cttctccggg
     6361 tagttggcct cgtttgtact gccgacccgc cggtgtcccc atcggacctt ggtcccatcg
     6421 tcaggagacg cgctgcgcac gacgcttgtg agacgtcggg cttcgccccg gaggtttgct
     6481 gtgaacaagt cgatcacccg tgccatcacc ctctcctgtc tgttcgccgt cgtcttcgca
     6541 tcggtcggca tggccaagga gaaaccaccg gaaaactggg atggtctcga gcgcaagaag
     6601 gtcaagggtc tcgacaacgt ctatgtgcgg cccaacgtgg agttcacgcc atacaagacc
     6661 gtgatgctcg atcccacgca ggtcgagttc tccaaacact gggagaagag ccaggacttc
     6721 cgcaatcgcc cgaccgcgga agacatgcag aagatcaagg acaagctcgc cgagctgatg
     6781 cacagcgtgt tcaccgagga actcgtggaa aacggctaca cggtggtcac caccgcgaat
     6841 gaagacacgc tgcaggtccg aaccgcgatc atcgacctgt tcatcaacgc tccggatctg
     6901 caggacccgg gcatcacgag atcgtatacg acgagtgccg gcgcgatgac actggtgctc
     6961 gaagcgcgcg acggaccgac cggtcaactg ctggcgcgcg cggtggatcg ccaggaagac
     7021 gaccacatgg gcgggtacat gatgtggacc aacgcagcca ccaacacggc cgacgcgcgg
     7081 cggatcatcc aggtgtgggc gaagaagctg cgcgagtccc tcgaccggct caacggcaag
     7141 gagccgaaga agaagtgagt cgcgagcgac tcagtcgaac caggcgtact gccaggcgac
     7201 tccccaggtg ctgaaatcgt tgccggtgcg ggtgctcgcc ccttcgctcc agctcgcctt
     7261 gatcgactgg cgcgctccta tcggcagcga gaacgtcacg cccagccggg agttctcctt
     7321 gcggtccgcc ttgagcacgc cgtccacggt cgattgcccg ccgaagtaat acgtgctgtc
     7381 gagcgcgatc catagtcgcg ggcggatcgt ataactcaca tgaccctgaa tcgtcgtgat
     7441 cggatcctgt gagcggcgca caccgccgaa gaattcatcg ttgtcggtga acagccagag
     7501 gccggcatag acatcgaaca tccagcgatt gtggggcatc gagaaaccga gctcgggttt
     7561 gaacgcccag cgattcgtgc cgatgttgat gagcttgtcg gagtagtact gacccgtggg
     7621 agcgatgacc acgaggctcg cgccgagcgt ggacctcggc gtgcgctgca tgaattcctt
     7681 cggggtcagc gcgggaccgc ccaggagatt cgtggcgagc cgaaggcgca catcgccgag
     7741 gccatcacga gtcacggccc ggcgttcttc acccacgtct cccgagacat caccccacac
     7801 atagggcacc gcaaccgcaa cgctcgccga ttggccccac agcgcgaacg tgcggccgta
     7861 gcctgcgaca ccggcgtcga tggtcgcctc gatgttctcg atgggcacgg acggatccgg
     7921 gaggatctcg cccgtcgatc gcgagccggc gaacagcacg aagttgagcc cgaccgggct
     7981 cggagaatag gcgcgcggct cgagctcctg cgccccga
//
LOCUS       DATDEU010000082         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1036612, whole genome shotgun sequence.
ACCESSION   DATDEU010000082 DATDEU010000000
VERSION     DATDEU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036612"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VIV63_01925"
     CDS             <1..742
                     /locus_tag="VIV63_01925"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY6123382.1"
                     /translation="DMAVGLSLLIGVRLPYNFNSPYQAWNITEFWRRWHMTLSRFLRD
                     YLYVPLGGNRHGRARRYVNLMLTMLLGGLWHGASWTFVIWGGLHGAYLVVNHGWQWLR
                     EKLGWKHAASGLAAILGRSASVALTLLCVMIGWVFFRAVDVASATGILASMFGLRDGG
                     LPITALAGYSHGIVPWIALFSLLALFGRNSQALIDGHLTAWLDRRAERPRHPEWLAGM
                     AGAMCVGVVFMAIAAASRTVTEFIYFNF"
     gene            748..1827
                     /locus_tag="VIV63_01930"
     CDS             748..1827
                     /locus_tag="VIV63_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY6123383.1"
                     /translation="MAVTPIITRALGFLAGIAGALVLLEVILRLLPVLEGTYAADPRP
                     SWPVHTMIPNSTFTYSTGWNLQNLQQGRINNYGYVAPFDYAPAHGAANDSIAVFGDSY
                     VEAQMNDYGDTLHGSLNEYLKTPRTVLNFGSAGAEMPDYLGVAPLVSREFSPQWAVFV
                     ITMGDFTRGFSAGPGYFKWQPDQNPPVKLIPEINRSASSKWLRTVALIRYLRGNLSLR
                     PDELIRLRRGADVAGVAGPCHREVLSKEDEVLLAAFARELPAALSLPPERVILIFDAD
                     RKAIYAGKTRAQARECQARAALANERLQELAAKGGMRVIDSYPVFQRHFADRKGPLDR
                     SPLDAHWNPAAHRLMAQEVARIIDP"
     gene            1975..2051
                     /locus_tag="VIV63_01935"
     tRNA            1975..2051
                     /locus_tag="VIV63_01935"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2009..2011,aa:Asp,seq:gtc)
     gene            2084..>3315
                     /locus_tag="VIV63_01940"
     CDS             2084..>3315
                     /locus_tag="VIV63_01940"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY6123384.1"
                     /translation="MLQRIRDGLQGQKWLAGFVLGAIGLTFVFWGGSGALDFTGMSGT
                     TAAEVDGVKIPAEEATRAWSDTQARWARQFGTDIPPEQRVAMQQNILDGLVLRKLVAK
                     RLDDAKFRVSDAKVLAEFQNIPSFQGPDGKFDVATARSVLAQNNLSENQFFEETRSQL
                     LMDQLQQGIGGSYFVTRGEQQRMFNLENEEREVQYAQFAADQFAGADPIDDAAIKAYY
                     DKNGDRFMSTESVALEFAELRLEQLATQVAPSDADLQKLYDENRGMYVLDERRRARHI
                     VITVNGDADAAALKQAETVAAEARAGKDFAELAKKYSNDATANDGGDLGFVQKQDFPS
                     PLGDTLFAMNVGDVSAPVKSQFGYHILKLEQIQAGEAKPFADVRAELDSQYRQDQAAE
                     LFGQRQETLASRIEKGAA"
BASE COUNT          578 a         1078 c         1054 g          605 t
ORIGIN      
        1 ggacatggcg gtcggcctga gcctgctgat cggcgtgcgg ctgccgtaca acttcaattc
       61 gccgtaccag gcgtggaata tcacggaatt ctggcgccgc tggcacatga cgttgtcgcg
      121 cttcctgcgc gactacttat atgtgccgct cggcggcaac cggcatggtc gcgcgcgccg
      181 ctacgtgaac ctcatgttga cgatgctgct gggcggcctg tggcacggcg cttcctggac
      241 tttcgtgatc tggggcggcc tgcacggcgc gtatctcgtc gtgaatcacg ggtggcaatg
      301 gcttcgcgag aagctcggct ggaaacacgc cgcctcgggc ctggccgcga tcctcggacg
      361 aagcgcgtcc gtggcgctga cgctgctatg cgtgatgatc ggctgggtgt tcttccgcgc
      421 cgtcgatgtg gcgtccgcca ccggcattct cgccagcatg ttcggacttc gcgacggcgg
      481 cctgccgatc accgcgctgg cgggttactc gcacggcatc gtcccgtgga tcgcgttgtt
      541 ctcgttgctg gccctgttcg gccgcaactc gcaagcgttg atcgacgggc atctgaccgc
      601 gtggctggac cggcgcgccg agcgcccccg ccatcccgag tggcttgccg gcatggccgg
      661 ggcgatgtgc gtcggcgtcg tgttcatggc gatcgccgcc gcgtcgcgca cggtcaccga
      721 attcatctac ttcaacttct aactaccatg gcggtcaccc cgatcatcac acgcgcgctc
      781 ggcttcctgg ccggcatcgc cggtgcgctc gtgttgctcg aagtgatcct tcgcctgttg
      841 ccggtgctcg aaggcaccta cgcggcggat ccgcggccgt cgtggccggt ccacacgatg
      901 atcccgaact cgacgttcac ctattcgacc ggctggaacc tgcagaacct ccagcaggga
      961 cgcatcaaca actatggcta cgtagcgccg ttcgactacg cgcccgcgca cggcgcagcc
     1021 aacgattcca tcgcggtgtt cggcgacagc tacgtggaag cgcagatgaa cgactacggc
     1081 gacacgctgc acggctcact caacgaatac ctgaagactc cgcggaccgt gctcaacttc
     1141 ggctccgccg gcgccgagat gccggactac ctcggagtcg ctccgctggt gagccgggag
     1201 ttctcgccgc aatgggcggt gttcgtgatc acgatgggcg atttcacacg tggcttttcc
     1261 gcgggcccgg gctatttcaa atggcagccg gatcaaaatc cgcccgtgaa gctgattccg
     1321 gaaatcaatc gctcggcctc gagcaagtgg ctgcgcacgg tcgccttgat ccgctacctg
     1381 cgcggcaacc tgtcactgcg gcccgatgag ctcatccgtc tgcggcgcgg cgccgatgtc
     1441 gccggagtgg ctggtccttg ccatcgcgag gttctgtcaa aagaagacga ggtgctgctc
     1501 gcggcgttcg cgcgcgaact tcccgcggct ctttcactgc cacccgagcg cgtcatcctg
     1561 atcttcgacg ccgaccgcaa ggcgatctat gccgggaaaa cccgggccca ggcccgggaa
     1621 tgccaggctc gcgcggccct cgccaacgag cggctccagg agctcgcggc gaagggtggc
     1681 atgcgggtca tcgacagcta tcccgtgttc cagcggcact ttgccgaccg caagggcccg
     1741 ctggatcgat cgccgctgga tgcccactgg aatcccgccg cgcatcgcct gatggcgcag
     1801 gaagtggcgc gaataatcga cccgtagaac ggggcaatgc atctcgcctg tacttttcag
     1861 attctctgct aaacttcgcg cccttccgac ggcctgcctg gcgggttcgt tggaaaggga
     1921 tcgccagtaa aaggcgaggg tcgagggccg acaccctcag cacccatcaa aattggagcg
     1981 gtagttcagt tggttagaat accggcctgt cacgccgggg gtcgcgggtt cgagtcccgt
     2041 ccgctccgcc aatcttttag gacccagtcg tttaggaaga tgaatgctgc agcgtattcg
     2101 tgatggcctc cagggccaga aatggctcgc cgggttcgtc ctgggtgcca tcggtctgac
     2161 gttcgttttc tggggtggat cgggtgcgct cgacttcacc ggcatgtcgg gcaccacggc
     2221 cgccgaagtc gacggcgtca agattcccgc ggaagaggcc actcgcgcct ggagcgacac
     2281 gcaggcgcgc tgggcgcgcc agttcggcac cgacattccg cccgaacagc gcgtggccat
     2341 gcagcagaac attctcgatg gcctggtcct gcgcaagctc gtggccaaac gcctggatga
     2401 cgcgaagttt cgcgtgagcg acgcgaaggt tctcgccgag ttccagaaca tcccgtcgtt
     2461 ccaggggccc gatggcaagt tcgacgtcgc caccgcgcgc tcggtgctgg cgcagaacaa
     2521 cctgtcggaa aatcagttct tcgaggaaac gcgctcgcag ctgctcatgg accagttgca
     2581 gcagggcatc ggcggctcct atttcgtcac gcgtggcgaa cagcagcgca tgttcaatct
     2641 cgagaacgaa gaacgcgaag tgcagtacgc gcagttcgcg gctgaccagt ttgccggcgc
     2701 cgacccgatc gacgatgccg ccatcaaggc ctattacgac aaaaacggcg accgcttcat
     2761 gagcaccgaa tcggtggctc tggagttcgc cgaactgcgc ctcgaacaac ttgcgacgca
     2821 ggtggcgccg tcggatgccg atctgcagaa gctttatgac gagaaccgcg gcatgtacgt
     2881 gctcgacgag aggcggcgcg cgcgccacat cgtcatcacg gtgaacggtg acgccgatgc
     2941 cgccgccctc aagcaggccg agaccgtggc cgccgaagca cgcgccggca aggatttcgc
     3001 cgagctcgcg aagaaatatt cgaacgacgc caccgcgaac gacggcggcg atctcggctt
     3061 cgtgcagaag caggacttcc cgagtccgct cggcgacacg ctgttcgcga tgaacgtcgg
     3121 cgatgtttcc gcgcccgtga aatcgcagtt cggttaccac atcctcaagc tcgagcagat
     3181 ccaggcgggc gaagccaagc cgttcgcgga cgtgcgcgcc gagctcgact ctcagtatcg
     3241 ccaggaccag gccgcggaat tgttcggcca gcgtcaggaa actctcgcca gccgcatcga
     3301 gaagggcgcg gctga
//
LOCUS       DATDEU010000083         3289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_890286, whole genome shotgun sequence.
ACCESSION   DATDEU010000083 DATDEU010000000
VERSION     DATDEU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890286"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VIV63_01945"
     CDS             complement(<1..403)
                     /locus_tag="VIV63_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6123385.1"
                     /translation="MGTNNIVLVHGLWMTPLSWEFWAHHYTERGYSVYAPSWPGMERD
                     VRALRRAPETYADLGFKRIVDHYEKLVIELESAPILIGHSFGGLVVQALLDRGLGACG
                     VAIASAPIKGIWTLPYSTMRVVTPQLINPRNN"
     gene            539..841
                     /locus_tag="VIV63_01950"
     CDS             539..841
                     /locus_tag="VIV63_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6123386.1"
                     /translation="MNEPTRQEIDASREPLVLEFGASWCGYCQAAQRDIANVLKAHPA
                     VRHVKVEDGKGKPLGRSFHVKLWPTLIYIEDGVEKGRVVRPANSVAIEAIFAGAKS"
     gene            838..1788
                     /locus_tag="VIV63_01955"
     CDS             838..1788
                     /locus_tag="VIV63_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123387.1"
                     /translation="MRGRCFRGGLAVALIFFFATTASAQSGADDADESAYAPSTLAKV
                     QGGAARSQNEEAELAPPGQWYTTTATFTGQKRQPSSRFRPLISSWVSSRGLQGDLVDM
                     FFSGDLPELQFEQAGKKYWVMAGLPGGLGFDVYPPGQKLTIYLQRVGFASGTPVTVLT
                     MAKLPSGVTSTNAAPAGRRMHNTRMGEGARRQEATFKKGSATFTYAGREYSLPLDVLG
                     RHSVAPGLYSYENGVRTALLEYGAGDDQYLLLRVPVADGSEAASLVPYLVLNGRIVPP
                     IDVGACAISLEKVQRAGAAGQVDCGDAPRALFSKLRFIAE"
     gene            complement(1795..2526)
                     /locus_tag="VIV63_01960"
     CDS             complement(1795..2526)
                     /locus_tag="VIV63_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY6123388.1"
                     /translation="MPGTAADVFNLAIKHQGEPYILGARAPLTNPQWKGPWDCAEFAS
                     WCLFQVTGVLFGVMPRSNPVTADAFTGFWADQAREANAIVPVGTAARTVGAFLVRKPA
                     TGKIGHIVISDGQGGTIEAHSSKTGVIRSSLANRRWDMGIIIPGVVAHAAPDLPPIEV
                     AVNTLRVQNPLIAAKIVEDVQRALIKLGFSPGRPDGVYGPQTAHAVRLFQVKKELVAD
                     GEVGPATFKALKIPVPAFVTKTVPL"
     gene            complement(2632..2883)
                     /locus_tag="VIV63_01965"
     CDS             complement(2632..2883)
                     /locus_tag="VIV63_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123389.1"
                     /translation="MPLPASAIDKLSDHSGSYVICVTCRQCQHEREMDPRSLANILGW
                     HVTLKQACTRLRCSRCQSRSFKVEIAFDRKPRGWKANPS"
     gene            2982..>3289
                     /locus_tag="VIV63_01970"
     CDS             2982..>3289
                     /locus_tag="VIV63_01970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6123390.1"
                     /translation="MPLKAAFIERCGALNQPGWLQMRVALWPDATPDEHRGYMAISLA
                     QPERFLQLMMYDDRRLPIGFIEGSIRSDYVNGTESSPVGFVEGVYVVPAMRRHGVARR
                     L"
BASE COUNT          586 a         1028 c         1066 g          609 t
ORIGIN      
        1 ggttgtttcg gggattgatc aactgcggcg tgaccactcg catcgttgaa tagggaagtg
       61 tccagattcc cttgatgggg gctgatgcga tggccacgcc gcaggctcct aacccacggt
      121 ctaacaaagc ctgaaccacg agtccgccga aggagtgccc gatgaggatc ggcgcgcttt
      181 cgagttcgat gaccagcttc tcgtagtgat ccacgatccg cttgaaaccg agatctgcgt
      241 acgtttccgg ggcgcgacgc aaggctcgaa cgtcgcgttc catgccaggc cagctcggcg
      301 catacacgct gtatccacgt tccgtgtagt gatgcgccca aaactcccag ctgagcggcg
      361 tcatccataa tccgtggacc aggacgatgt tgttagtgcc catgaggcct ccacaagaat
      421 cgtgaggttc cgcccgcttc ctgtcgttgt ccagcgacag ggaaagaaga tgtacgtagg
      481 agaatccagc aacgatcgct aacaacacac gcctcggctt ttcacctagg attgcgcaat
      541 gaacgaaccg acgcgccagg aaattgacgc atcccgcgag cctctcgtgc tcgaattcgg
      601 cgccagctgg tgtggctact gccaggcggc gcagcgcgac atcgccaacg tgttgaaggc
      661 gcaccccgcg gtgcggcacg tcaaggtgga agacggcaag ggtaagccac tggggcgctc
      721 gttccacgtg aagctgtggc ccacgctcat ttatatcgag gacggggtgg agaaggggcg
      781 cgtcgtgcgc cccgccaata gcgtcgcgat cgaagccatc ttcgccgggg cgaaatcttg
      841 aggggccggt gtttccgcgg cggcctggcc gttgcactga tcttcttctt cgcgactacc
      901 gcgtcggcgc agagcggtgc cgacgatgcc gatgaatctg cctacgcgcc gtccaccctc
      961 gccaaggtcc agggtggcgc tgcgcgctcg caaaacgagg aagcggagct cgcgccgccg
     1021 gggcagtggt acaccaccac cgccacgttc accgggcaga agcgccagcc gtcgagccgc
     1081 ttccgtccgc tcatcagttc gtgggtgagc agccgcggcc tgcaaggcga tctcgtggac
     1141 atgttcttca gcggcgacct gccggagctg cagttcgaac aagcgggcaa gaaatactgg
     1201 gtcatggcgg ggctgcccgg tgggctggga ttcgatgtct atccgcccgg ccagaagctg
     1261 actatctacc tgcagcgcgt gggtttcgcg tccggcacac ccgtgaccgt gctcaccatg
     1321 gcgaagcttc cttccggtgt caccagcacg aatgccgcac ccgccggccg ccgcatgcac
     1381 aacacacgca tgggcgaggg cgcgcgccgc caggaagcca cgttcaagaa gggttccgcc
     1441 acgttcacgt acgcgggtcg cgaatattca ctgccgctcg atgtgctggg ccgccattcg
     1501 gtcgcgccgg ggctgtactc ctacgagaac ggcgtccgta cggcgctgct cgagtacggc
     1561 gcgggcgacg atcaatatct gctgctgcgg gttcccgtcg ccgacggcag cgaagcggcg
     1621 agcctcgtgc cctatctcgt gctcaatggc cgcatcgtgc cacccatcga tgtcggggcg
     1681 tgcgcaatca gtttggagaa agtgcagcga gctggcgccg ccggccaagt ggactgtggc
     1741 gacgcgccgc gtgcactgtt ctccaaactg cgtttcattg ccgagtagtc atcctcaaag
     1801 cggaacggtc ttcgtcacaa acgccggcac cggaattttc aacgccttga acgtcgcagg
     1861 gccgacttca ccgtcggcca ccagctcttt cttgacctgg aaaagccgaa cggcgtgcgc
     1921 ggtctgcggg ccatagacgc cgtcgggccg cccgggcgaa aagccgagct tgatcagggc
     1981 gcgctgtacg tcttcgacga ttttcgcggc gatgaggggg ttctgtacgc gcagtgtgtt
     2041 gaccgccacc tcgatcggcg gcaggtcggg cgccgcgtgc gcaacgacgc caggaatgat
     2101 gatgcccatg tcccagcggc gattggcgag cgacgagcgg atcacgcccg tcttgctgct
     2161 atgggcttcg atggtgccgc cctggccatc ggagatgacg atgtggccta tcttccccgt
     2221 cgcgggcttg cgtaccagga aggcgccgac ggtccgggcc gcggttccca cgggcacgat
     2281 cgcattggcc tcacgcgcct ggtccgccca gaagcccgtg aacgcatccg cggtgacggg
     2341 gttgctgcgc ggcatgacac caaacaacac gcccgtgacc tggaacaggc accaggacgc
     2401 gaactccgcg cagtcccagg ggcctttcca ctgcgggttg gtcagcggcg cgcgtgcgcc
     2461 gaggatgtac ggctcgccct ggtgcttgat ggccagattg aaaacgtcgg ctgcagtgcc
     2521 tggcacggtc gctctccctg tgcttcatgt gggtctgact attcaactgt cgtgaagccg
     2581 ggagttatgg cacccgatcg gcgaaaattt ttcgggagtg ggagttgtcg atcaggacgg
     2641 gttggctttc catccgcgcg gcttgcgatc gaaggcgatc tccaccttga agctgcggga
     2701 ctggcacctg gagcagcgca ggcgagtgca ggcctgcttc aacgtgacgt gccagccgag
     2761 gatgttagct agggagcgtg gatccatttc gcgctcgtgc tggcattgcc ggcaggtgac
     2821 gcagatgacg taagaccctg agtggtctga gagtttgtcg atggcgctgg cggggagggg
     2881 catggacgga agtttttccg gcccggcgcg gaacgtccac ccagtacccg gcgagtcggg
     2941 acactgtatg aaaccgagcc cagcctgcta ccctccatgt catgcccctt aaggccgcct
     3001 ttatcgaacg ttgtggtgca ctgaaccagc ccggctggtt gcagatgcgc gtggcgctgt
     3061 ggccggacgc cacccccgac gagcatcgcg gctacatggc aatttccctt gcccagccag
     3121 agcgtttcct gcaactcatg atgtatgacg accggcgcct gcccatcggt ttcatcgaag
     3181 gctcgattcg cagcgactac gtcaacggta cggaatcatc gcccgtcgga ttcgtcgagg
     3241 gcgtgtacgt ggtgcccgcc atgcggcggc acggcgtggc acgccgcct
//
LOCUS       DATDEU010000084         3024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1328777, whole genome shotgun sequence.
ACCESSION   DATDEU010000084 DATDEU010000000
VERSION     DATDEU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328777"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..2038
                     /locus_tag="VIV63_01975"
     CDS             410..2038
                     /locus_tag="VIV63_01975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6123391.1"
                     /translation="MMSEYGSAPLDSAEIRSDLQDRLARFARDTFLISGGMLLLSLGV
                     DISNGYGSLATLFVQAPRVAHVICTLALLAGWRTCRGQAASIQLLEAMDAMLTILLCI
                     GWAMLGVGMAPANPIEFGIILAFTHTLIGRSVIVPSSYQRTLWISACSALPVLSVFIY
                     RQMAFIPGASASFVRSFLVFSSIWCLVAVVVATLNSRQIFGLRRQIREIGKLGQYTLE
                     DKIGEGGMGVVYRAAHAMLRRPAAIKLLLPERSSERDLARFEREVQLTSQLYHPNTIS
                     IFDFGRTAGGTFYYVMEYLDGVDLERLVREAGPLEPARVIRILVQACGSLAEAHALGL
                     IHRDIKPANIILTERADESDIVKVVDFGLVKTMVASSDPAVTNVNAITGTPLYLSPES
                     FTSPETVDGRADLYALGAVGYFLLTGKRVFDGASVVEVCSKHLMETPVPPSERVGRPM
                     PDDLQSVILSCLAKEASDRPASARALRAQLLACADASRWDAEAARTWWRDEGTRIRAQ
                     KTIPPTREGSSAIVIDLRGRAARTEQIVIDPSAQ"
     gene            complement(2073..2327)
                     /locus_tag="VIV63_01980"
     CDS             complement(2073..2327)
                     /locus_tag="VIV63_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY6123392.1"
                     /translation="MEDAARSWAEEVFGQADLGDSRRTRRLIETHEDVHEVVSGYTQR
                     WKIEELHRTWKSGACRVEETQLRTADAVMKWAIITAANTR"
     gene            2543..>3024
                     /locus_tag="VIV63_01985"
     CDS             2543..>3024
                     /locus_tag="VIV63_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123393.1"
                     /translation="MRHEFSLRFEPASGAKFVQAQAYVRALEAALEGLSSLRAALAPE
                     LAAKHGWPLAQVRVALTFSIGPTAKGSLIVPLVAGGGDKGPQLASDAIAQAFWKETGL
                     ELGRVAKGQAARLSATGAEAFARASAAAKDSSAKLSFATKTARGTWKSVAAITPLEPA
                     L"
BASE COUNT          480 a          972 c          982 g          590 t
ORIGIN      
        1 tatgcgttca ttgccgaatc tgtcaatgcg cgtgagacag cggtcacgag agttctatgc
       61 ggaagcgttc ctgtttcggt agatggcgcg ggcggcgtcc tcgccgcgac gtgaggcgcc
      121 tttctgtgtt tggggttcgg atggtgtgtc ggtgactttc ggcgcgttca tgttttgcag
      181 cggtgccggc cgactcgcgc gactgacaaa gttcgtcgag gcttcgagcg gtgatgtgca
      241 ccgttgaggg cggcagggcg gccttgggcg ggccgttcgg gaaccgttcg ggtgtcgagc
      301 gcgtgcggcg tcgacggctt ccgtggcgcg cggcgtgtac tagcccccag attccggtcc
      361 ggcagccgcc ttttcgggcc gcgtgccggc catggtattg aggcagcaga tgatgtccga
      421 gtacgggagc gcgcctctcg acagcgccga gatccgcagc gatttacaag atcgcctggc
      481 gcgcttcgcc cgagacacgt tcttgatctc cggcggcatg ttgctgctgt cgcttggcgt
      541 cgacatctcc aacgggtatg ggtcgctcgc gacgctgttc gtgcaggccc cccgggtcgc
      601 gcacgtcatc tgcacgctgg cattgctcgc cgggtggcgc acctgccgcg gccaagccgc
      661 gagcatccag ctactcgaag cgatggacgc gatgctgacc atcctgctgt gcatcggctg
      721 ggcgatgctc ggggtgggga tggcccctgc caatccgatc gagttcggga ttatcctcgc
      781 cttcacgcac acgttgatcg ggcgctcggt gatcgtgccg agctcgtacc agcgaaccct
      841 gtggatcagc gcctgctccg ccctgcccgt actgtcggtc ttcatctacc gccagatggc
      901 gttcattccg ggcgcctcgg ccagtttcgt tcgcagcttt ctggtcttca gctcgatctg
      961 gtgcctggtg gcggtcgtgg tcgcgacgct caactcgagg caaatcttcg gcctccgtcg
     1021 gcagatccgc gaaatcggca agctcgggca gtacacgctc gaggacaaga ttggcgaggg
     1081 cggcatgggc gtcgtgtacc gcgccgctca cgccatgttg cgtcgcccag ccgccatcaa
     1141 gctcttgttg cccgaacgct cgagcgagcg cgacctggcg cgcttcgaac gcgaggtgca
     1201 gctcacgagc cagctctatc acccgaacac gatctcgatc ttcgatttcg ggcgcaccgc
     1261 gggcggcacg ttctactacg tgatggagta cttggacggc gtggatctgg agcgcctggt
     1321 gcgcgaagcg ggaccgctcg agcccgcgcg cgtgattcgt atcttggtgc aagcctgtgg
     1381 ctccttggcc gaggcccacg ccctcggctt gattcatcgc gatatcaagc ctgccaacat
     1441 catcctcacc gaacgcgccg acgagtcgga tatcgtgaaa gtggtggatt tcgggttggt
     1501 gaagaccatg gtggcctcgt ccgacccggc cgtcaccaac gtcaacgcga tcactggtac
     1561 gccgctctac ttgtcccccg agtcgttcac gtcccccgag accgtcgatg ggcgcgccga
     1621 cctgtacgcg ctgggcgcag tcggctattt tctgctgacc ggcaaacgcg tgttcgacgg
     1681 cgcgtcggtc gtggaggtgt gcagcaagca cctgatggag acgcccgtgc ccccgtcaga
     1741 gcgcgtcggg cgccccatgc cggacgacct gcagagcgta attctgagct gcctggccaa
     1801 ggaggcgagt gaccgtcctg catccgcaag ggcgctgcgc gcccagctat tggcctgcgc
     1861 ggatgcaagc cgttgggatg ccgaggcggc gcgtacctgg tggcgtgacg aaggcacccg
     1921 cattcgcgcg cagaagacca tccctccgac ccgcgaaggc tcgagcgcga tcgtcatcga
     1981 cctacgtggg cgcgccgcac gcaccgagca aatcgtgatc gatccaagtg ctcagtgagt
     2041 gctcagcgcc acgtagcttc acctcatccc gttcaccgag tgttggcagc cgtgatgatc
     2101 gcccatttca tcacggcgtc cgcggtacgc agctgagttt cttccacgcg gcaggccccc
     2161 gacttccagg ttcggtgcaa ctcttcgatc ttccagcgct gcgtgtatcc gctgactact
     2221 tcatgtacgt cttcgtgggt ttcgataagg cgtcgcgtgc ggcggctatc tccaagatcc
     2281 gcctgcccga aaacttcttc tgcccagctg cgtgcggcat cttccatgct gcaacaaccg
     2341 ctgccgttct tcgaaattcc gagagatgtg acagatccga tcaatggtca gacggagtcg
     2401 ggggaacttc atgtcgcccg tcgcgcgacc gcaagccggt gtaccgcgag agagacgcac
     2461 aacgggcccg gcttcctcgc gtccggcgac gcactgacag aaaattgcct agccagcggg
     2521 cgtttccagt taagattgtc ggatgcgcca cgaattttcc ctccgcttcg agcccgcttc
     2581 aggcgccaag ttcgtgcagg cgcaggccta cgtgcgcgcg ctcgaagctg cgctcgaggg
     2641 actaagttcg ttgcgggcgg cgctcgcgcc cgagctggca gcaaagcacg gctggccact
     2701 ggcgcaggtc cgcgtcgcgc tcacgttctc gatcggcccc actgccaagg gcagcctgat
     2761 cgtcccgttg gttgccggcg gtggcgataa gggcccgcag ctggccagcg atgccattgc
     2821 acaggcgttt tggaaagaga ccggcttgga gctcgggcgc gtggcgaagg gccaggcagc
     2881 acgactgagt gcgacgggcg ctgaggcttt cgcgcgcgcg agtgcagcag ccaaggacag
     2941 ctcagcgaaa ctgagcttcg ccaccaaaac ggcgcgcgga acctggaaat cggttgccgc
     3001 catcacccct ctcgagcccg cgct
//
LOCUS       DATDEU010000085         5891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_161406, whole genome shotgun sequence.
ACCESSION   DATDEU010000085 DATDEU010000000
VERSION     DATDEU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161406"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..817
                     /locus_tag="VIV63_01990"
     CDS             116..817
                     /locus_tag="VIV63_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123394.1"
                     /translation="MNFVPAPRRVSPRRALIVSAIAMVMPSRIRRWLLRVLCGYSFGE
                     GARIGCSLIGCTSLQMGPSARIGHFNIVKGVRVDLAECATVGDFNWISGLGAGDTKHF
                     TEETGRDPAFVMGRHAAITSRHYIDCSNRVDIGEFATVGGARSQILTHAIDFKRNRQV
                     SAPVRIGRYAFVGTSCVVLKGAQLPDCSVLAAGSSLARAYDDTFTLYSGVPATPVKAL
                     DRDSEYFRRVRGYVA"
     gene            834..2039
                     /locus_tag="VIV63_01995"
     CDS             834..2039
                     /locus_tag="VIV63_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6123395.1"
                     /translation="MNPLNIFVVHPSDLLTDHLPNGAGWIDYNYLRGLAERGHTLHVA
                     TPRAELRGPLPPRMHLHRIPGTDVRRPGPLTRLRYVLGVRKLFKTLSKTVRFDLAQQF
                     TPVETGLSLALLGSGVPLILGPYSGHWAAEAFGPPKPKSFLARMKHHVRDLLASFQQS
                     QADALVITCPAAIERVRSPLARTTRVHVISHGIHSQEYLPRESMPLKPTILFLAVLEY
                     WKGIFTLLDAFDRVAPEVPDATLEIWGDGRESEAVDARIAKSPFSDRIFRRGRAARED
                     VAQIMRAHSVYCMPSYGEPFGMTLLEAMASAVPVVTTNIGGPPFLVHRDGGRVVPMRD
                     VVALADALTEILSDRELQASMGSYNRRRVEQEFDWSRSLDRMESVYAQVLRPQREAAS
                     DALPDTRWT"
     gene            2057..2839
                     /locus_tag="VIV63_02000"
     CDS             2057..2839
                     /locus_tag="VIV63_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY6123396.1"
                     /translation="MEISASIDPPAKAPGFRKSIKFALIRLFGGSLEEMMFQWRRLSM
                     RVNTWLGRPYTALYGMDRKLEKYLNFEGGTFIEAGANDGIAQSNSYFLENKYGWNGLL
                     VEPVPKYFRMCQSARGVNVVNCGLGPFEKDGEELEILAGGLMSLPVSVDEKLLHGRSV
                     RQHASIGAREFGGIAPELVRTRVRALSNVLDELKITVVDFFSLDVEGFELEVLKGLDM
                     TRHAPRYLLIETEQLEAVTEFLGSRYEMIEALSHHDFLFRRI"
     gene            complement(2844..4757)
                     /locus_tag="VIV63_02005"
     CDS             complement(2844..4757)
                     /locus_tag="VIV63_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6123397.1"
                     /translation="MNTAANSKVTNQKIVVVDDNPASLYSTSRILRSAGFEVIEATTG
                     AQALAAADKDIGLIVLDVNLPDIDGLEVCRRLRARSTTAYLPIVHRTATFVAHDDHQQ
                     GLSAGGDSYLTHPVDPPVLIATVRALLFARQADIVRRTTDARFRTVFELASSGIALLD
                     PDLVYRDVNPEFCRIAGRDRAAIVGKGCIDIIAPSHHTLCRTLHQVLADGGRWEGTMP
                     VQRPDGSVADVEWRIVAENGHGARIAIATNVTERERLLASERAARTEAERSNQIKDEF
                     LATLSHELRNPLNAMLGWATVLKRKNVTPAMLTQGLDAIERNSRVQSHLIEDLLDFAG
                     IRFGKMRIEVDAIPPARAIEAAVEVVSPQARQKEVQIHLEVYDRDSHVMADDSRLQQI
                     IWNLLSNAIKFTPSGGRIDVTARAEDGSYEIAVTDSGRGISAEFLPQIFERFSQQESG
                     TGKSFAGLGIGLTIVKHLVDIHHGSIEVESDGVGKGATFRVKLPLTDTRPSAIPTDQN
                     TRLRKMRILVVEDDSDARALISRILTDAGAQVSDVASADAAMTHIKANVPDLLVSDIG
                     MPVLDGYQLLRNVRSAGFDASRLPAIALTAFSRLQDRADAIDAGFQMHLAKPVRAEAL
                     LAAVSSLTQRKAT"
     gene            complement(4754..5614)
                     /locus_tag="VIV63_02010"
     CDS             complement(4754..5614)
                     /locus_tag="VIV63_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6123398.1"
                     /translation="MSAELQAQLAAREADIALLREELEETNKGVVALYAELDDKAAEL
                     REATELKSRFLSYMSHEFRTPLTSMTSIAGILLSRLDGPLTPEQQKQVEFIRSSTREL
                     TDMVGDLLDLAKVEAGRITISAEWFEMVDLFSALRGMFKPIVASTTISLVFDEPQGDI
                     KLYTDDKKLSQILRNFISNALKFTSDGEVRVTATLLPGERVEFAVIDTGIGIAAEHIP
                     NLFADFVQLDVRLQKRLRGSGLGLSLARKFATLLGGEVRAESELGKGSRFSVTLPMNL
                     SAARAPSVSA"
     gene            complement(5614..>5891)
                     /locus_tag="VIV63_02015"
     CDS             complement(5614..>5891)
                     /locus_tag="VIV63_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEY6123399.1"
                     /translation="GNISGTLLSLDKSQGMVSHNGTLGLNQRRSQQFEYQRHPGALLI
                     MHSDGVSARWELKQRPDLLNCHPAIIAAALYRDHGRDRDDSTVVVVR"
BASE COUNT         1002 a         1879 c         1929 g         1081 t
ORIGIN      
        1 gcgcgcgccg cctcatgacg ttcgaggcgt cgggggccgc gtccgtacgc aggctttcgg
       61 caggcgcctc gcggctggac gtcggcgaca acctcagcat cgtggacatc gcgcgatgaa
      121 cttcgtaccc gcaccacgcc gagtcagccc acgccgcgcg ctcatcgtgt cggcaatcgc
      181 gatggtcatg ccctcgagga tccgacggtg gctgctgcgc gtgttgtgtg gctattcgtt
      241 tggtgaaggc gcacgcatcg gctgctcgct catcggctgc acgtcgctgc agatggggcc
      301 ctcggcgcgt atcggccact tcaacatcgt gaagggagtg cgcgtcgatc tcgcagaatg
      361 cgccaccgtc ggcgatttca actggatctc gggactcggc gcgggcgata ccaaacattt
      421 caccgaagag actggtcgcg atccggcatt cgtcatgggc cggcacgcgg cgatcacctc
      481 gcggcactac atcgactgct cgaatcgcgt cgacatcgga gaattcgcca cggtgggtgg
      541 cgcacgatcg cagatcctca cgcatgccat cgacttcaaa cgcaatcggc aggtgtcggc
      601 gccggtgcgc atcggccgct atgcgttcgt gggtacatcc tgcgtggtgc tcaagggcgc
      661 ccaactaccc gactgctcgg tgctggcagc gggttcgtcg cttgcgcgcg cctacgacga
      721 cactttcacg ctgtactcgg gggtccccgc gacgccggtg aaggcactcg accgcgattc
      781 cgagtatttc cgccgcgtgc gcggttacgt tgcatgaata gaaggagcgc cgcatgaatc
      841 cgctgaacat cttcgtggtc cacccctcgg acctcctgac cgaccacctg ccgaacggcg
      901 ccgggtggat cgactacaac tacctgcgcg ggcttgccga gcgcggccac acgctgcacg
      961 tggccacacc gcgtgccgag ctgcgcgggc ctttgccgcc gcgcatgcac ctgcaccgga
     1021 ttcccggcac cgacgtgcgc cgtcccgggc cgctcacccg gctacgctat gtcctcggcg
     1081 ttcgaaaact cttcaagacc ttgagcaaga ccgtgcgctt cgacctcgcg cagcagttca
     1141 cgccggtgga gacgggcctg agtctcgcgc tcctcggcag cggagtgccg ttgatactgg
     1201 gaccgtactc cggtcactgg gcggcggagg ccttcgggcc cccgaagccg aagagttttc
     1261 tcgcacgcat gaaacaccac gtgcgcgacc tgctggcgtc attccagcag agccaggcgg
     1321 atgcactggt catcacgtgt cccgcggcca tcgagcgcgt ccgttcgccg ctggcgcgca
     1381 cgacgcgcgt gcacgtcatc agccacggca ttcacagcca ggaatacctg ccgcgcgagt
     1441 cgatgccgct caagccgacc atcctgttcc tggccgtgct cgaatactgg aaaggcatct
     1501 tcacgttgct cgacgcgttc gatcgcgtgg cgccggaagt gcctgacgcg acgctcgaga
     1561 tctggggcga tggccgcgag agtgaggcgg tcgacgcgcg tatcgcgaag tcgccgttca
     1621 gcgatcgcat cttccgccgt ggccgcgcgg cgcgtgaaga cgtggcgcag atcatgcgcg
     1681 cgcactcggt gtactgcatg ccgtcgtatg gcgagccttt cggcatgacg ctgctcgaag
     1741 ccatggcgtc cgcggtgccg gtcgtcacga cgaacatcgg cgggccaccg tttctcgtgc
     1801 atcgcgacgg cgggcgcgtg gttcccatgc gcgacgtcgt ggcccttgcg gatgcgctca
     1861 ccgaaatcct gtccgatcgc gaactgcagg cgagcatggg cagctacaac cgccgccgcg
     1921 tcgagcagga gttcgactgg agccgctcgc tcgatcgaat ggaaagcgtt tatgcccagg
     1981 tgctccgccc gcagcgggaa gccgcctccg acgcattgcc cgatacgcgc tggacctgag
     2041 aatgaaagga acacccatgg aaatttccgc aagcatcgat ccgccggcga aggcacccgg
     2101 cttccgcaag tccatcaagt tcgcgctgat ccggctcttc ggcggatcgc tcgaagagat
     2161 gatgttccag tggcggcgcc tgtccatgcg cgtgaacacg tggttaggcc gcccgtatac
     2221 cgcgctgtac ggcatggacc gcaagctcga gaagtatctg aacttcgagg gcggaacgtt
     2281 catcgaggcc ggcgccaacg atggcatcgc gcaatccaac agctacttcc tcgagaacaa
     2341 atacggctgg aacggcttgc tggtcgaacc cgtgcccaaa tatttccgca tgtgccagag
     2401 cgcgcgcggc gtgaacgtcg tcaactgcgg actcggaccg ttcgagaagg acggcgagga
     2461 actggagata ctcgccggcg gactcatgag cctgccggtg agcgtggatg aaaagctgct
     2521 gcatggccgc tccgtgcggc agcacgcatc catcggcgcg cgagaattcg gcggcatcgc
     2581 gcccgagctc gtgcgcacgc gcgttcgcgc gctgtcgaac gtgctcgacg aactgaagat
     2641 caccgtcgtc gatttctttt cactcgacgt cgaaggcttc gagctcgagg tgctcaaggg
     2701 gctcgatatg acccgtcatg cgccgcgtta cctgctcatc gaaaccgaac agctggaagc
     2761 cgtcacggaa ttcctggggt cgcggtacga aatgatcgag gcgctctcac accacgattt
     2821 cctgttccgg cggatctgag gtctcaggtc gcctttcgtt gggtgaggct ggagacggcg
     2881 gccaacagcg cctccgcgcg caccggcttg gccaggtgca tctgaaagcc ggcgtctatc
     2941 gcgtcggcgc gatcctggag ccgtgagaac gcggtgagcg cgatcgcggg cagccgcgag
     3001 gcgtcgaagc ccgcgctgcg cacattgcgc aggagctggt agccatcgag caccggcatg
     3061 ccgatgtcgc tcaccagcag gtcgggcaca ttggccttga tgtgcgtcat ggcggcatcc
     3121 gcactggcga cgtcgctgac ctgagcgccg gcatccgtga ggatgcgcga gatgagtgca
     3181 cgcgcatccg agtcgtcttc caccacgagg atgcgcatct tgcgcagccg cgtgttctga
     3241 tccgtcggaa tggcgctggg ccgcgtatcc gtcagcggca gcttgacccg gaacgtggcg
     3301 cccttgccca ccccgtcgct ttccacctcg atggacccat ggtgaatgtc gacgaggtgt
     3361 ttgacgatgg tcaggccgat tccgagcccc gcgaaactct tgccggtgcc ggactcctgt
     3421 tgactgaagc gttcgaagat ctgcggcagg aactcggcgc tgatgccacg gccactgtcg
     3481 gtgaccgcga tttcatagga cccgtcttcg gcgcgcgccg tgacgtcgat gcgtcctccc
     3541 gatggcgtga acttgatcgc gttgctcagc aggttccaga tgatctgctg caggcgcgaa
     3601 tcgtccgcca ttacatgcga gtcccgatcg taaacctcga ggtggatctg cacctccttc
     3661 tggcgcgcct gcggactcac gacttcgacc gcggcttcaa tggcgcgcgc gggcggaatg
     3721 gcgtccactt cgatgcgcat cttgccgaag cggatgccgg cgaagtcgag caggtcttcg
     3781 atgagatggc tctgcacgcg tgagttgcgc tcgatggcgt cgaggccctg ggtcagcatc
     3841 gccggcgtca cgttcttgcg cttgagcacg gtggcccagc caagcatcgc attcaggggg
     3901 ttgcgaagtt cgtgcgacaa cgtcgcgagg aattcatcct tgatctggtt gctgcgctcc
     3961 gcctcggtgc gcgcggcgcg ttcgctcgcc agaaggcgtt cgcgttccgt gacattggtg
     4021 gcgatcgcaa tgcgcgcgcc atggccgttc tcggccacga tgcgccattc cacgtcggcg
     4081 acactgccgt cgggacgttg cacgggcatc gtgccttccc atcggccgcc gtcggcgagg
     4141 acctggtgaa gcgtcctgca gagcgtgtgg tgcgacgggg caatgatgtc gatgcagccc
     4201 ttgccgacga tggccgcgcg atcacggccg gcgatgcggc agaactcggg attcacgtcg
     4261 cgatagacga ggtcggggtc gagcagggcg ataccgctcg aggccagctc gaatacggtt
     4321 cgaaatcgcg cgtcggtggt gcggcggacg atgtccgcct ggcgcgcgaa cagcagcgca
     4381 cgcaccgtgg cgatgagcac cggcgggtcg actggatgcg tgagataact gtcgccgcct
     4441 gccgagagcc cctgctgatg atcgtcgtgg gcgacgaacg tcgccgtccg gtgcacgatg
     4501 ggtagatagg ccgtcgtgct gcgcgcgcgc agccggcggc agacttccag gccatcgatg
     4561 tcgggcagat tgacgtcgag cacgatgagc ccgatgtcct tgtcggcggc ggccagcgcc
     4621 tgcgcgccgg tcgtcgcttc gatgacctca aatcccgcgg atctcaggat gcgcgaggtg
     4681 gaatacagcg acgcgggatt gtcatccacc accacgatct tctggttagt caccttgctg
     4741 ttggcggccg tgttcatgcg gataccgacg gcgcacgtgc ggccgacagg ttcatgggca
     4801 gagtcaccga gaagcgcgaa cccttgccga gctcactttc cgcgcgcact tcgccgccga
     4861 gcagcgtcgc aaacttgcgc gccagcgaaa gaccgagacc cgagccccgc aggcgtttct
     4921 gcaggcgcac gtcgagctgc acgaagtcgg caaacaggtt gggaatgtgt tcggcggcga
     4981 tgccgatgcc ggtgtcgatg accgcgaatt cgacacgctc gccgggcagc aacgtggcgg
     5041 tgacgcgcac ttcgccatcg gacgtgaact tgagcgcatt ggagatgaag tttcgcagga
     5101 tctgcgaaag tttcttgtcg tcggtgtaca gcttgatgtc cccttgcggc tcgtcgaata
     5161 ccagcgaaat cgtcgtgctc gcgacgatgg gcttgaacat tccgcgcaga gccgagaaca
     5221 ggtccaccat ctcgaaccat tccgcggaga tggtgatgcg cccggcttcc accttggcga
     5281 gatcgagcag gtcgccgacc atgtcggtga gttcgcgcgt ggagctgcgg atgaattcca
     5341 cctgcttctg ttgttcggga gtcagcggac cgtcgaggcg cgacaacagg atgccggcga
     5401 tgctggtcat ggaggtgagc ggcgttcgga actcatggct catgtacgag aggaaacggc
     5461 tcttgagctc cgtcgcttcg cgcaactccg ccgccttgtc gtcgagctcg gcgtacaacg
     5521 cgaccacgcc cttgttggtc tcttcgagtt cttcgcgcag caatgcgatg tcggcttcgc
     5581 gggccgcgag ctgcgcctga agttcggcgg acatcagcgc accaccacca cggtcgaatc
     5641 gtcgcggtcg cggccatgat cccgatagag ggccgccgcg atgatggccg gatggcaatt
     5701 gaggaggtca ggccgctgct tcagctccca gcgtgcggat acgccatccg aatgcatgat
     5761 cagcagcgcg cccggatggc gctgatattc gaactgctgg gaccgccgct ggttcagccc
     5821 caaggtgccg ttgtgcgaca ccatgccctg cgatttgtcg agcgatagca gcgtcccgga
     5881 gatgttgccc a
//
LOCUS       DATDEU010000086         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1766444, whole genome shotgun sequence.
ACCESSION   DATDEU010000086 DATDEU010000000
VERSION     DATDEU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766444"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VIV63_02020"
     CDS             <1..681
                     /locus_tag="VIV63_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY6123400.1"
                     /translation="IYSAKMLFEAFKSLLKLAILGGVSVAFFFALWPQLQGAESADTT
                     AVQNWFGTNAVTLLFRLGLALVLIGLLDFAYTRHKFGKDMMMSRREVKEEVKRREGDP
                     LIRAKLRELQRENLKQSASVKRVPEADVLITNPDHLAIALKYERGTMSAPIVLAKGAD
                     CWAADMRAMARRHGIPIYERKPLARLLFRIGQLGQFIPADTYVDVARLYADLEAKRRG
                     AAEYEVRA"
     gene            678..>2279
                     /locus_tag="VIV63_02025"
     CDS             678..>2279
                     /locus_tag="VIV63_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY6123401.1"
                     /translation="MSFWRRFFGEQTELALVALLGGILLVLFAPIPPGLLDFLLIANF
                     SFALLILLLTFYMGRPLEFSTFPSLLLVATLFRLSLNIAATRLILAEAHAGQVIEAVG
                     NQVVGGNYVIGLIVFLVLIIVQFVVVTNGAQRVAEVAARFTLDSMPGKQMSIDADLNM
                     GLIDQNEARDRRKQIEKEANFYGAMDGASRFVKGDAIAGILIILVNIIGGLTIGVAQH
                     DMAWGEALSTYTLLTVGDGIVTQVPALIIATATGIIVTRAASDARLGAEVSKQVMAYP
                     RVIAIVTVVLVALAFMPGLPAWPVLIIACGMGALFWYAHRKGEAAAEPEKAKADAKAA
                     ETDDLYQTLKVDPVDITIGSELIPLVGEEGKLLSEKLGTIRKQFALDMGVVLPPVRVR
                     DDKRMGAARYEIRIFGSRVADGEVHPDRLLAINPGGPRPALDGVTAMDPAYGLPAVWI
                     AEANRAAARAGGYTVVDAAMVFLTHVAEVLRQNAQNFITRAETERLIARVREAQPALV
                     DDLVPKTLSFADLQRVLQNLVRERVP"
BASE COUNT          374 a          718 c          783 g          404 t
ORIGIN      
        1 atctacagcg caaagatgct attcgaggcg ttcaagagcc tgctcaagct ggccattctc
       61 ggcggcgtgt cggtggcgtt tttcttcgcg ctgtggccgc agctgcaggg ggcggagtcc
      121 gcggatacca ccgcggtgca gaactggttc gggacgaatg cggtgacgtt gctgttccgg
      181 ctgggattgg cgctggtgct catcggcctg ctcgacttcg cctatacgcg ccacaagttc
      241 ggcaaggaca tgatgatgag ccggcgcgag gtcaaggaag aggtcaagcg ccgcgagggc
      301 gatccgctga tccgcgcgaa gctgcgcgaa ctgcagcgcg agaatctcaa gcagtccgct
      361 tcggtgaagc gcgtgcccga agcggatgtg ctcatcacca accccgatca cctggcgatc
      421 gcgctcaagt acgagcgcgg cacgatgtcg gcgccgatag tgctggccaa gggcgcggat
      481 tgctgggcgg cggacatgcg cgcgatggcg cggcgtcacg gcatcccgat ctacgaacgc
      541 aaaccgctcg cgcgactcct gttccgtatc gggcagttag gccagttcat ccccgccgac
      601 acctacgtcg acgtggcccg tctgtacgcg gatctcgagg cgaagcgccg gggtgcggcg
      661 gagtacgagg tgcgcgcatg agtttctggc ggcgcttctt cggcgaacag acggagctcg
      721 cgctggtcgc tctgctgggc ggcatcctgc tggtgctgtt tgcgccgatt ccgccgggtc
      781 tcctggattt cctgctcatc gcgaatttct cgttcgcgct gttgattctc ctgctcacgt
      841 tctacatggg ccggccgctc gagttctcga cgttcccgtc gctgctgctg gtggcgacgt
      901 tgttccggct cagcctcaac atcgcggcga cgcgcctcat cctggcggag gcccacgcgg
      961 gccaggtcat cgaggcggtg ggcaaccagg tcgtcggcgg aaactacgtc atcggtctca
     1021 tcgtgttcct ggtgctcatc atcgtgcagt tcgtggtggt cacgaatggc gcgcagcgag
     1081 tcgccgaagt ggcggcgcgc ttcacgctcg actccatgcc tggcaagcag atgagcatcg
     1141 atgcggacct caacatgggg ctcatcgatc agaacgaggc gcgtgaccgc cgcaaacaga
     1201 tcgagaagga agcgaacttc tacggcgcaa tggacggcgc cagccgtttc gtgaagggcg
     1261 acgccatcgc cggcatcctc atcatcctgg tcaacatcat cggcggtctc accatcggcg
     1321 tggcgcaaca cgacatggcg tggggcgaag cgctgtccac ctacacgctg ctgaccgtgg
     1381 gcgacggcat cgtcacacag gtgccggcgc tgatcatcgc gacggcgacc ggcatcatcg
     1441 tcacgcgcgc ggcatcggat gcacgcctgg gcgccgaggt gtcgaagcag gtcatggcgt
     1501 atccgcgcgt gatcgcgatc gtgacggtgg tgctggtggc gctcgcgttc atgccgggcc
     1561 ttccggcctg gccggtgctg atcatcgcct gcggcatggg cgcgttgttc tggtacgcgc
     1621 accgcaaagg cgaagcggcc gcggagcccg agaaggccaa ggccgacgcc aaggcggcgg
     1681 aaacggacga tctctaccag acgctcaagg tggatccggt ggatatcacc atcggcagcg
     1741 aactcatccc gctcgtgggc gaagagggca agctgctctc cgaaaaactg ggcacgatcc
     1801 gcaagcagtt cgcgctcgac atgggcgtgg tgctgccgcc ggtgcgcgtg cgcgacgaca
     1861 agcgcatggg cgcggcgcgc tacgagatcc gcatcttcgg atcgcgcgtc gcggatggtg
     1921 aggtgcaccc cgaccggttg ctggccatca acccgggcgg cccgcgaccg gcgctcgacg
     1981 gtgtgaccgc gatggatccg gcctatggcc ttcccgcggt gtggatcgcc gaggcgaatc
     2041 gtgcggcggc gcgtgctggc ggctacacgg tggtcgatgc ggccatggtt ttcctcacac
     2101 acgtggccga agtgctgcgc cagaacgcgc agaacttcat cactcgcgcg gagacggagc
     2161 gcctgatcgc acgcgtgcgc gaggcgcagc ccgcactcgt cgacgacctg gtgccgaaga
     2221 cgctgtcatt cgccgacctg cagcgggtcc tgcagaacct cgtgcgtgag cgtgtgccg
//
LOCUS       DATDEU010000087         7788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_307540, whole genome shotgun sequence.
ACCESSION   DATDEU010000087 DATDEU010000000
VERSION     DATDEU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307540"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VIV63_02030"
     CDS             <1..1363
                     /locus_tag="VIV63_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY6123402.1"
                     /translation="MSGSGYVMGMAIASYEWMAALTLIVVGKWILPVFLKNGIYTMPE
                     FLEKRYSPAVRNVMAVFWLALYIFVNLTSILWLGSLAVNAIAGLDIQVAITCLAAFAL
                     LYALYGGLKAVALTDIVQVSLLVLGGIIITYMSLDKVSGGAGVIAGFKILTTQYPEKF
                     DMILSPDNPHYKDLPGLAVLLGGMWMMNVSYWGFNQYIIQRGLAAKSVNEAQKGIVLA
                     AFLKLLMPAIIVLPGIAALVLVPGLEKTDQAYPSLMAMLPIGIKGLVFAALVAAIVAS
                     TGSKINSIATIFTMDLYRAARPDTSQKNLVLVGRIVAVVSVIIAVIITKPFLSNFGQG
                     FQYIQNFTGFVTPGICVIFLLGLFWARTTATAAMVSMLATVVISWVFYKYLPDYPFMN
                     RVGWCFVAGIVIAVAISLMTPKSEAAMRVDITNVDFKTGTGFNISALLIVAILAVLYY
                     TWW"
     gene            complement(1368..2321)
                     /gene="ubiA"
                     /locus_tag="VIV63_02035"
     CDS             complement(1368..2321)
                     /gene="ubiA"
                     /locus_tag="VIV63_02035"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY6123403.1"
                     /translation="MANQSLTDLDLTPPPSDARLTYLRYTRRIRDLIEVARLHRPIGI
                     WLLLWPVLWAVWIAGASHPTPKVLVIFVLGTVAMRSAGCVINDLADRDFDPFVKRTRD
                     RPIASRRLAPVEALVWFFVLIAIALALVLQLDPATIRMSFIGAALAVSYPFFKRFFPL
                     PQFYLGVAFSWGVPMAFMAQLGEIPRVAWIIFFAGIIWAAIYDTMYAMVDRDDDLKIG
                     VKSSAIVFGDMDKVIIGTLQALMLYALYLVGTSMDYGYWYNMGLVGAGVFFVYQQWRI
                     RERKPQSCFDAFLNNHYVGLSVFVGILLEYAFRVPEQVLFQ"
     gene            complement(2353..3033)
                     /locus_tag="VIV63_02040"
     CDS             complement(2353..3033)
                     /locus_tag="VIV63_02040"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6123404.1"
                     /translation="MAVQILNDMPDAAVHRGDLHDFSVRAQQTPRAPFRVSGRAVFMA
                     ATIGVHLIVGAAFLSIRHELEVSAPEPIVATLMESSDAQPETPRDLTPPQVNVVYSLT
                     VPQELSFESEAPAPEVITAAISQTTPQSIAPPMIESVEYVRAPAPKYPAESQRHRERG
                     IVLLRVMVDVLGRPAEIQVERSSGFVRLDDAARAAVEKALFHPHVVNGIAQPAQVLIP
                     IEFARRAS"
     gene            complement(3121..5217)
                     /gene="recG"
                     /locus_tag="VIV63_02045"
     CDS             complement(3121..5217)
                     /gene="recG"
                     /locus_tag="VIV63_02045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY6123405.1"
                     /translation="MATPAPQPTYEQRPVASMRGVGPALAEKLEKLGVTQVQDLLFVL
                     PSRYEDRTQVAEIGSLRHGTRVVVEGEIQLTEVAYRRRRQLLCRISDGTGSLTLRFFY
                     FSGAQQANLARGVRIRCYGEVRRGPLGLEIVHPEYRRIGAEAAPLEEVLTPIYPTTEG
                     VPQARLRSLIDIALRDTEASGIRDWIPPAMLADLELPSLLDALAMMHRPPRDAHLGEL
                     LSGHHPAQRRLAFEELLAHQLSLRLLRQEIKSDPAWRLDDASGLEKRFLDSLPFKLTA
                     AQSRALAEVDQDLTDDRPMVRLVQGDVGCGKTVVAAAAAARAVGGGLQAALMAPTELL
                     AEQHAKNFERWFRALEIPVALVTGSLPARTRRSALEAVRNGDIKLVVGTHALFQESME
                     FEKLGLVIVDEQHRFGVHQRLSLRDKGARGGKLPHQLIMTATPIPRTLAMTAYADLDV
                     SVIDELPPGRTPVKTVVLPETRRDEIVQRIHKAVLEGRQAYWVCPLIDESEELRYQAA
                     EETAAALAEALPEVRVGLVHGRMPSREKERGMYAFKEGTIGLLVATTVIEVGVDVPNA
                     TLMVIENAERMGLAQLHQLRGRVGRGAHESHCVLLYGGKLSEMARARLAAIRETNDGF
                     VIARRDLELRGPGELLGTRQTGLAEMRVADLMRDSDLLPAVQSAAEILLKSNPDAVQG
                     LTRRWVGSGERYGRIG"
     gene            complement(5230..5619)
                     /locus_tag="VIV63_02050"
     CDS             complement(5230..5619)
                     /locus_tag="VIV63_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6123406.1"
                     /translation="MGSVRQAIHTDQAPKAIGPYSQAVRVGDTVYMAGQIPLDPVTMQ
                     MVEGDFEKEAARVFENIKAVIAASGGTFRQVVKVTIFITDFANFAKVNELMAKYFTEP
                     YPARSTVAVAALPRNARVEIECTLHLG"
     gene            complement(5639..7771)
                     /locus_tag="VIV63_02055"
     CDS             complement(5639..7771)
                     /locus_tag="VIV63_02055"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY6123407.1"
                     /translation="MPGLRELINSIEAYLPAEQVERVRSAAEFGAEAHEGQKRLSGEP
                     YIAHPLAAAQILADLHLDPDTIIAAILHDVIEDTPISKEELSAKFGAPVAEIVDGVTK
                     LDQIQFKSRAEAQAESFRKMVLAMVRDLRVILVKLADRTHNMRTLDAMAPARRRAVAR
                     ETLDIYAPIAGRLGLYNMKLELEDLGFKAAFPHRYKVLDRALKKARGNQKQFLNKIAE
                     TMRVALVKADLKTDVSTREKHLYSIYSKMLRKAAPLSEIVDVFGLRIVVDRADTCYRA
                     LGVVHAAYKPMPGRFKDYIAIPRVNGYQSLHTTLFGPNGIPIEVQIRTEDMHRVAEAG
                     IAAHWKYKVNETEGDQDARAREWLANLVDMQEGGSSEDFLESVKVDLFPDKVYVFTPK
                     GEILRLPRGATVVDFAYAVHTDVGNRCVAAKVDRRLTPLRTPLRNGQTVEIITAKGAM
                     PNPSWVNFVVTAKARAAVRHYLKGLRRQEAIELGARLINQALGEFRLSLEEVAADTLN
                     AAVVELGMHDVDELYEKVGLGERLAPLVARRLLPTKASEESFSGAPAPLAIAGTEGLL
                     VAYARCCFPIPDDPIFAFLSAGRGVVVHRENCVNVEDYRKHPENWLPVAWQATTDKVF
                     SSEIRVDVANKMGVLAAVAAAISSGDTNIERVAVEEKDADSSSLVFELRVRNREHLAR
                     VVKVIRRMPEVLKVTRTIASHGRSNSRD"
BASE COUNT         1349 a         2453 c         2551 g         1435 t
ORIGIN      
        1 catgtcgggt tccggatacg tgatgggcat ggccatcgcg tcgtacgagt ggatggccgc
       61 gctcacgctg attgtcgtcg gcaagtggat cctgccggtg ttcctcaaga acggcatcta
      121 cacgatgccg gagttcctcg agaagcggta cagcccggcg gtgcgcaacg tgatggccgt
      181 cttctggctt gcgctctata tcttcgtgaa tctcacgtcg atcctgtggc tgggctcgct
      241 cgcggtgaat gccatcgccg gcctcgatat ccaggtcgcc atcacctgcc tcgcggcgtt
      301 cgcgctgctc tacgcgctgt acggcggcct caaggccgtg gctctcaccg acatcgtgca
      361 ggtgtcgctg ctggtgctcg gcggcatcat catcacgtac atgtcgctcg acaaggtcag
      421 cggtggcgcc ggcgtcatcg cgggcttcaa gatactcacg acgcagtatc ccgagaagtt
      481 cgacatgatc ctgtcgcccg acaatcctca ctacaaggat ctgccgggac tcgccgtgtt
      541 gttaggcggc atgtggatga tgaacgtctc gtactggggc ttcaatcaat acatcatcca
      601 gcgtggcctc gccgccaaga gtgtgaacga ggcgcagaag ggcatcgtgc tcgcggcgtt
      661 cctcaagctg ctgatgcccg cgatcatcgt gcttcccggc atcgccgcgc tggtgctcgt
      721 gcctggcctc gagaagaccg accaggccta tccgagtctc atggcgatgc tgccgatcgg
      781 catcaagggc ctggtgttcg cggcgctggt cgcggccatc gtcgcgtcga ccgggtccaa
      841 gatcaattcg atcgcgacca tcttcacgat ggacctgtat cgcgccgcgc ggcccgacac
      901 ctcgcagaag aacctcgtcc tggtgggccg catcgtcgcg gtcgtgtcgg tcatcatcgc
      961 ggtcatcatc acgaagcctt tcctcagcaa cttcggccag ggcttccagt acatccagaa
     1021 cttcaccggg ttcgtcacgc cgggcatctg cgtgatcttc ctgctgggcc tcttctgggc
     1081 ccgcaccacg gcgacggcgg cgatggtgtc gatgctggcg acggtcgtga tctcgtgggt
     1141 gttctacaag tacttgcccg actatccgtt catgaatcgc gtgggctggt gcttcgtcgc
     1201 cggcatcgtg atcgcggtgg ccatctcgct gatgacgccg aagtcggaag ccgcgatgcg
     1261 cgtcgacatc acgaatgtcg acttcaagac cggcacgggg ttcaacattt ccgcgctgct
     1321 gatcgtcgcg atcctcgcag tcctctacta cacgtggtgg tagtggctta ctgaaacagc
     1381 acctgctcgg gtacgcggaa cgcgtattcg agcaggatgc cgacgaacac cgacaacccc
     1441 acatagtggt tgttgaggaa ggcgtcgaag caggattgcg gcttgcgctc gcggatgcgc
     1501 cactgctgat agacgaagaa cacccccgcg ccgaccagcc ccatgttgta ccagtagccg
     1561 taatccatgc tcgtgccgac caggtacagc gcgtagagca tcagcgcctg cagcgtgccg
     1621 atgatcacct tgtccatgtc gccgaagacg atggcgctgg atttcacgcc gatcttgagg
     1681 tcgtcgtcgc gatcgaccat ggcgtacatg gtgtcgtaga tggcggccca gatgattccc
     1741 gcaaagaaga tgatccacgc gacgcgcgga atctcgccga gctgcgccat gaaggccatg
     1801 ggcacgcccc agctgaacgc gacgcccagg tagaactgcg gcagcggaaa gaagcgtttg
     1861 aagaacggat aactcaccgc gagcgccgcg ccgatgaacg acatgcggat ggtggccgga
     1921 tcgagctgca gcaccagtgc cagtgcgatg gcgatcagca cgaagaacca gaccagcgct
     1981 tcgaccggcg ccaaccgccg cgaggcaatg gggcggtcgc gcgtgcgctt cacgaaaggg
     2041 tcgaaatccc gatcggcgag atcgttgatc acgcaaccgg ccgaacgcat ggcaaccgtg
     2101 cccagcacga agatgacgag caccttaggt gtgggatgcg atgcgccggc gatccacacg
     2161 gcccacaaca cgggccagag cagcagccag ataccgatgg gacgatgcag gcgcgcgact
     2221 tcgatgaggt cgcggatgcg ccgcgtgtaa cgcagatagg tcagccgcgc gtccgacggc
     2281 ggcggggtga gatccaggtc ggtgagagac tggttagcca ctggcgcgca tcatatacgc
     2341 gccgcggtgt tttcagctcg cgcggcgtgc gaactcgatc gggatcagca cctgcgcggg
     2401 ctgcgcgatg ccgttcacca cgtgcggatg gaacagcgcc ttttccacgg cggcgcgcgc
     2461 ggcatcgtcg agacggacga agccgctgga tcgctcgacc tggatttccg ccgggcggcc
     2521 gagcacgtcg accatcacgc gaagcagtac gattccgcgt tcgcgatggc gctgggattc
     2581 ggcgggatat ttcggcgcgg gcgcgcgcac gtattccacc gattcgatca tcggtggcgc
     2641 aatggactgc ggggtggttt gcgaaatcgc cgcggtgatg acttcgggtg ccggcgcttc
     2701 gctctcgaaa gacagttctt gcggaacggt cagtgaataa acgacgttga cctgcggcgg
     2761 cgtgaggtcg cgcggagttt ccggctgggc gtccgacgat tccatcagcg tggcgacgat
     2821 gggctcgggc gcgctgacct cgagctcgtg acgaatgctc aggaacgctg cgccgacaat
     2881 gagatgaacg ccgatggttg ccgccatgaa gacggcccgt ccggaaacgc ggaacggcgc
     2941 gcgcggcgtc tgttgcgcgc gaacggagaa gtcgtgcagg tcaccgcggt gaaccgcggc
     3001 gtcaggcatg tcgttgagga tttgtacggc catgggtcgg gctacgaatg taaatgattc
     3061 gcatttggag ctcaacgccg attcttatct atgtgcggca ggtccgcctt cccgaacggc
     3121 ctacccgata cgcccgtatc gctccccgga tcccacccac ctgcgcgtaa gtccttgtac
     3181 cgcatcggga ttgctcttga gcaggatctc ggcggcggac tgaacggctg gcaggaggtc
     3241 cgaatcgcgc atcaggtcgg ccacgcgcat ttcagcgagg ccggtttgcc gggtcccgag
     3301 aagctcgccg ggaccccgca gctccaggtc gcgacgcgca atcacgaacc cgtcgttggt
     3361 ctcgcgaatg gccgcgagcc gcgcgcgcgc catctcggag agcttgccgc cgtacaacaa
     3421 cacgcaatga ctttcgtggg cgccgcgacc cacgcgtcct cgcaactgat gcagctgagc
     3481 gagtcccatc cgctcggcgt tctcgatgac catcagcgtg gcgttcggta cgtcgacgcc
     3541 cacttcgatg accgtcgtgg cgactaacaa cccgatcgtg ccttccttga atgcgtacat
     3601 gccgcgttct ttctcgcgcg aaggcatgcg gccgtgaacg aggcctacac gcacctcggg
     3661 cagcgcttcg gcgagcgcgg ccgcggtttc ctcggcggcc tgatagcgca gttcttcgga
     3721 ctcgtcgatg agcgggcaga cccaatacgc ctggcggcct tcgaggaccg ccttgtgaat
     3781 acgctgcacg atttcgtcgc ggcgggtctc tggcaatacc accgtcttca cgggcgtgcg
     3841 gcccggcggc agttcatcga tgaccgatac gtcgaggtcg gcgtatgccg tcatggccag
     3901 cgttcgcggg atcggcgtgg cggtcatgat gagctggtgc ggaagcttgc cgccgcgcgc
     3961 gcccttgtcg cgcaggctca ggcgctgatg cacgccgaag cgatgttgct cgtcgacgat
     4021 cacgagcccg agcttctcga actccatcga ctcctggaac aacgcatgcg tacccaccac
     4081 gagcttgatg tcgccgtttc tcacggcttc gagcgcacta cgccgcgtgc gcgccggcag
     4141 tgagccggtg accagtgcga cggggatttc cagcgcgcgg aaccagcgtt cgaagttctt
     4201 cgcgtgctgt tcggccagca attccgtggg cgccatgagt gcggcctgga ggccgccacc
     4261 gaccgcacgc gcggcggccg cagcggccac gacggttttc ccgcagccca catctccctg
     4321 caccagccgg accatggggc ggtcgtcggt caggtcctga tccacttccg cgagcgcgcg
     4381 cgattgcgca gccgtgagct tgaaaggaag cgaatccagg aaacgctttt cgagtccgct
     4441 cgcgtcgtcg agtcgccagg caggatcact cttgatctcc tggcgtagca gtcgcaacga
     4501 aagctggtgg gccagcagtt cctcgaaggc cagacgccgc tgcgccgggt ggtggccgct
     4561 caggagctcg ccgaggtgag cgtcacgcgg aggccgatgc atcatcgcga gcgcgtcgag
     4621 caatgaggga agctcgaggt cggcgagcat cgccggagga atccagtcgc gaatgccgga
     4681 ggcttcggtg tcgcgcagcg caatgtcgat gagcgaacgg agtcgtgcct gcggcacgcc
     4741 ttccgtggtc gggtagatag gtgtcagcac ttcctcgagc ggcgcggcct cggcgccgat
     4801 gcggcgatac tcgggatgca cgatttcaag gcccagcgga ccgcgccgca cttcgccgta
     4861 acagcggatg cgtacaccac gggcaagatt ggcctgctga gcgcccgaga aatagaagaa
     4921 gcgcagcgtg agcgaaccgg tgccgtcgga gatccggcac aggagctggc ggcggcggcg
     4981 atacgccact tcggtgagct ggatttcccc ttccaccacg acgcgcgtgc cgtggcgcag
     5041 gctgccgatc tcggcgacct gggtgcggtc ctcgtagcgc gagggaagta cgaacagcag
     5101 gtcctggacc tgcgtgacac cgagcttttc gagtttttcc gccagcgccg ggccgacgcc
     5161 acgcatggac gcgaccgggc gctgctcgta ggtaggctgc ggcgcgggcg ttgccacctt
     5221 agcgggggat caacccaggt gcagcgtgca ctcgatctcg acgcgcgcgt tgcgcggcag
     5281 ggccgcgacc gccaccgtcg agcgcgccgg gtagggctcg gtgaaatact tcgccatgag
     5341 ttcgttgacc ttcgcgaagt tggcgaagtc ggtgatgaag atggtgacct tcacgacctg
     5401 gcggaacgta ccgcccgacg cggcgatgac ggccttgatg ttttcaaaca cgcgagcggc
     5461 ttctttctcg aaatcgcctt ccaccatctg catggtgacc gggtcgagcg gaatctgacc
     5521 ggccatgtac accgtgtcgc cgacgcggac cgcctgcgaa tagggcccga tggccttcgg
     5581 cgcctgatct gtatgaatgg cctggcgaac gcttcccatc acgaaactcc tgtggaaatc
     5641 agtcgcgcga gttgctgcga ccgtggctgg cgattgtacg cgtgaccttg agcacttcgg
     5701 gcatccggcg aatgactttc acgacgcgcg ccaggtgttc gcggttgcgc acgcgcaatt
     5761 cgaaaaccag cgatgagctg tcggcatcct tctcttcgac ggccacacgt tcgatattcg
     5821 tatcgcccga cgagatggcg gccgccaccg ccgcgagtac acccatcttg ttggcgacat
     5881 cgacgcggat ctccgagctg aacaccttgt cggtggtcgc ctgccaggcg accggcagcc
     5941 agttctcggg atgtttgcga tagtcctcga cgttcacgca gttctcgcga tgcacgacca
     6001 cgccgcggcc cgcggagaga aacgcgaaga tcggatcgtc gggaatcggg aaacagcacc
     6061 gcgcataggc gaccagcaaa ccttcggtgc ccgcgatggc aagaggcgcg ggcgcgcccg
     6121 agaaggattc ctcgctggcc ttggtgggca ggaggcggcg cgccaccagg ggtgcgagtc
     6181 gttcgcccaa acccactttt tcatagagtt cgtcgacgtc gtgcatgccg agttcgacga
     6241 cggcggcatt gagcgtgtcg gcagcgacct cttcgagcga caggcggaat tcacccagcg
     6301 cctggttgat gagccgcgcg ccgagctcga tcgcttcctg gcggcgaagc cccttgaggt
     6361 agtgacgcac cgcggctcgc gccttcgcgg tgaccacgaa gttcacccac gacggattcg
     6421 gcatggcgcc cttggccgtg atgatctcga ccgtctggcc gttacgcagc ggcgtgcgca
     6481 gcggcgtcag ccgtctatct accttggccg cgacgcagcg attgccgacg tcggtgtgaa
     6541 ccgcgtacgc gaaatcgacc acggtggcgc cgcgcggcag gcgcaggatc tcgcccttgg
     6601 gcgtgaacac gtacaccttg tcggggaaca ggtcgacctt gacgctctcg aggaaatcct
     6661 cggagctgcc gccctcctgc atgtcgacga ggttggcgag ccattcgcgc gcacgcgcgt
     6721 cctggtcacc ttcggtctcg ttgaccttgt atttccagtg cgcggcgatg ccggcctcgg
     6781 cgacgcgatg catgtcctcg gtgcggatct gcacttcgat cgggatgccg ttgggcccga
     6841 acagcgtggt gtgcaacgac tggtagccgt tcacgcgcgg gatcgcgatg tagtccttga
     6901 accggcccgg catcggcttg taggccgcat gcaccacgcc caacgcgcga tagcaggtat
     6961 ctgccctgtc taccacgatg cgcagaccga acacgtcgac gatctccgac agcggcgcgg
     7021 ctttgcgcag catcttgctg tagatgctgt acagatgttt ctcgcgcgtg ctgacgtcgg
     7081 tcttgagatc cgccttcacc agcgccacgc gcatggtctc ggcaatcttg ttcaggaact
     7141 gcttctggtt gccgcgcgcc ttcttcagcg cgcggtccag caccttgtag cggtgcggga
     7201 acgctgcctt gaagccgaga tcttcgagct cgagcttcat gttgtagagg ccgagacgcc
     7261 cggcaatggg tgcgtagatg tcgagcgtct cgcgcgccac cgcgcgtcgg cgggccggcg
     7321 ccatggcatc gagcgtgcgc atgttgtgcg tgcgatcggc gagcttcacg aggatcacgc
     7381 gcaggtcgcg gaccatcgcc agcaccatct tgcgaaagga ctcggcctgg gcttcagcgc
     7441 gcgacttgaa ctggatctgg tcgagcttgg tgacgccgtc gacgatctcg gctaccggtg
     7501 cgccgaattt cgccgacagc tcctccttgg agatcggcgt gtcttcgatg acgtcgtgca
     7561 ggatcgcagc aataatagtg tctggatcca ggtgcaggtc cgcgaggatc tgcgcggcgg
     7621 cgagcgggtg cgcaatgtac ggctcgccgg agagccgctt ctggccctcg tgcgcttcgg
     7681 cgccgaattc agcggccgag cgaacgcgct cgacctgctc ggccggtaaa taagcttcga
     7741 tgctgttgat cagctcgcgc aggcccggca gtcgccgggc actgggca
//
LOCUS       DATDEU010000088         2819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_15866, whole genome shotgun sequence.
ACCESSION   DATDEU010000088 DATDEU010000000
VERSION     DATDEU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15866"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VIV63_02060"
     CDS             <1..639
                     /locus_tag="VIV63_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123408.1"
                     /translation="WADGHLRTQVNAFYNDYENFQVTVGYPDIPVFGFELNNPNSTEI
                     YGFEAQLEAVFGAFTLDAGLGWLQSSLGEFYAVDPRAISFGACDPLTGPESAACINLE
                     GKDQTYAPELTFNIGMQYDFTLGEGNTLSPRINYGHVSEQWATLFQNEARGDRVEARD
                     IVNAQVAWTLNTFVTTLYGTNLTDQHYVGAINSGLRFAGAPRQFGLRIAKTF"
     gene            642..1943
                     /locus_tag="VIV63_02065"
     CDS             642..1943
                     /locus_tag="VIV63_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6123409.1"
                     /translation="MNTAVESIHTRPGARAWAVLAALCFVYVLNFLDRQLLSILAKPI
                     QDDLGVTDGQIGLISGLYFALFYCLLAIPVGWLADRSNRVRVLSFACALWSAATVACG
                     LSSNYAQLAAARMSVGVGEAGGVPPSYAIISDYFPPGTRGTALGLFNLGPPLGQAFGV
                     AFGASIAAAYSWRSAFIALGAIGIATALAVWLFVREPTRGGLDGPARGRAQTQPKAEF
                     WATCRKFFTHPVLLRVALACGATQFVTYAILNFTTLFLMREKGMTLKEVAVYYALMIG
                     VTMCAGMLASGRLIDRFATRSRTAYAWLPATALAIAIPFFIGFAWAPGWKLALLLLAV
                     PMFLNYFYLSPSVALVQEAVQPHERVLAGALLLLVMNLIGLGLGPTFLGAASDYFRAE
                     HPANSLQMAFYTLVPFYALAVASFVWLARALKRESTIGVAP"
     gene            1940..>2819
                     /locus_tag="VIV63_02070"
     CDS             1940..>2819
                     /locus_tag="VIV63_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY6123410.1"
                     /translation="MISNRFCVPGLLVAAILSLPATASDAPVINAPAGKVSGISAGTL
                     QVFKGIPYALPPTGIRRWKPPLPMPKWKDTRVATQYGPACVQPKARSGSIYAWDIGAL
                     SEDCLTLNVWAPAKAGKAPVFVWIHGGALSTGSGGEPMYDGSKLAERGLVVVTINYRM
                     GILGFLAHPALSAESRRNVSGNYGLLDQIAALRWVKNNIAAFGGDAGNVTIAGESAGG
                     LSVMYLMVAPEARGLFHKAVAQSAYMISAPELRGTTYGGVAAEPVGPWLGEKLGAGDL
                     AGLRSMDAVALTEGAAS"
BASE COUNT          419 a          909 c          950 g          541 t
ORIGIN      
        1 tgggccgacg gccacctgcg cacgcaggtg aatgccttct acaacgacta cgagaatttc
       61 caggtgaccg tgggttatcc ggacatcccg gtattcggct tcgagctcaa caacccgaac
      121 tccacggaga tttacggctt cgaggcccag ctcgaggcgg tgttcggcgc gttcacgctg
      181 gatgcgggcc tcggctggtt gcagagctcg ctcggagagt tctacgcggt cgacccgcgc
      241 gccatcagct tcggcgcctg cgaccccttg acgggtccgg aaagcgccgc gtgcatcaat
      301 ctcgagggca aggatcagac ctatgcgccc gagctcacgt tcaacatcgg catgcagtac
      361 gacttcacgc tgggcgaagg caacacgctc tcgccgcgca tcaactacgg ccacgtgtcg
      421 gagcagtggg caacgttgtt ccagaacgag gcgcgcggcg atcgcgtcga ggcgcgtgac
      481 atcgtgaacg cgcaggtggc gtggacgctc aacaccttcg tgacgacgct ctacggtacc
      541 aacctcaccg accagcacta cgtgggcgcg atcaattcgg gtctgcgttt cgcgggtgcg
      601 ccgcgccagt tcggcctgcg catcgcgaag acgttctgaa ggtgaacacc gcggtcgagt
      661 cgattcacac acgcccgggc gctcgcgcct gggcggttct cgcggcgttg tgcttcgtgt
      721 acgtgctgaa cttcctcgac cggcagttgc tgtcgattct cgccaagccc atccaggatg
      781 atcttggggt gaccgacgga cagatcgggc tcatcagtgg cctgtatttc gcgctgttct
      841 attgcctgct cgcgatcccc gtgggctggc tcgcggatcg ctccaatcgc gtgcgcgtgt
      901 tgtcgttcgc ctgcgcgttg tggagtgcgg cgacggtggc ctgcggcctc tcctctaact
      961 acgcgcagct ggcagctgcg cgcatgtcgg tgggtgtggg cgaggcgggc ggcgtgccgc
     1021 cgtcgtacgc catcatctcg gactactttc cgcccggcac gcgcggcacg gcgctcgggt
     1081 tgttcaatct cggtccgccg ctggggcagg cgttcggcgt ggccttcggc gcgtcgatcg
     1141 cggcggcata ttcctggcgc tcggcgttca tcgcgctggg ggcgatcgga attgcgacgg
     1201 cgcttgccgt gtggctgttc gtgcgcgagc ccacgcgagg cgggctcgac ggccccgcgc
     1261 gtggacgagc gcaaacgcag cccaaggcgg aattctgggc cacctgccgc aagttcttca
     1321 cgcatccggt gctgctgcgc gtcgcgctgg cctgcggcgc gacgcagttc gtcacctacg
     1381 ccatcctcaa cttcaccacg ctgttcctga tgcgcgagaa aggtatgacg ctcaaggaag
     1441 tcgcggtgta ctacgcgttg atgatcggcg tgacgatgtg cgccggcatg ttggcctcgg
     1501 gccgtctcat cgaccgcttt gcgacgcgct ccagaaccgc gtacgcgtgg ctgccagcca
     1561 ccgcgctcgc catcgcgatc cccttcttca tcggcttcgc ctgggcgccg ggctggaagc
     1621 tggcgcttct gttgctcgcc gtacccatgt tcctgaacta cttctatcta tcgccttcgg
     1681 tggcgctggt gcaggaagcg gtgcagcccc acgagcgcgt gctggcgggt gcattgctgc
     1741 tgctggtgat gaatctcatc ggccttgggc tcgggcccac gttcctgggc gcggccagtg
     1801 attatttccg cgccgaacat ccggcgaatt ccctgcagat ggctttctat actctggtgc
     1861 cgttctacgc gctcgccgtc gccagcttcg tgtggctggc gcgcgcgctg aagcgcgaat
     1921 ccacgatcgg agtcgcccca tgatctcgaa caggttttgc gtcccgggtt tgttagtcgc
     1981 ggccatcctg tcattgccgg cgaccgccag cgatgccccg gtcatcaacg cacccgccgg
     2041 aaaagtgtca ggcatctccg cgggcacgct gcaggtgttc aagggcattc cgtacgcgtt
     2101 gccgcccacg ggaattcgcc gctggaagcc gccactgccc atgccgaaat ggaaggacac
     2161 gcgcgtcgcc acgcagtacg gcccggcctg tgtccagccc aaggcgcgca gcggcagcat
     2221 ctatgcgtgg gacatcggcg cgctcagcga agactgcctc acgctgaatg tctgggcgcc
     2281 ggcgaaagcc ggcaaggcgc cggtgttcgt gtggattcac ggcggggcgc tgtcgaccgg
     2341 ttctggcggc gagccgatgt acgacggctc gaagctcgcc gagcgcgggc tcgtcgtggt
     2401 caccatcaat taccgcatgg gcatcctggg cttcctcgcg cacccggcgc tgagcgcgga
     2461 gtcgcggcgc aacgtgtcgg gcaactatgg cctgctggat cagatcgccg cgttgcgctg
     2521 ggtgaagaac aacatcgccg cgttcggcgg cgacgcgggc aatgtcacga tcgcgggtga
     2581 atccgcgggc gggctcagcg tcatgtacct catggtcgca cctgaagcgc gaggcctgtt
     2641 tcacaaggcc gtggcgcaga gtgcgtacat gatctccgcg cccgagttgc gcggtacgac
     2701 gtatggcgga gtagcggcgg agccggtcgg gccgtggctc ggcgagaagt tgggtgcggg
     2761 cgacctcgcg ggcctgcgtt cgatggacgc ggtggcattg accgaagggg cggcttccg
//
LOCUS       DATDEU010000089         3367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1037760, whole genome shotgun sequence.
ACCESSION   DATDEU010000089 DATDEU010000000
VERSION     DATDEU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037760"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1966)
                     /locus_tag="VIV63_02075"
     CDS             complement(95..1966)
                     /locus_tag="VIV63_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6123411.1"
                     /translation="MIEVDSAQREARAREVRHDVVATLTVAAHRLFHFVFAVQWAVAV
                     VLAWKSSFPGEPRIVFTMILGAVLSVPGLLFARAAPQASWVRHYLAACQIGWSMLFIW
                     LLEGQPEAQLHVFMSLAFLAFYRDWRVLVTATVIAIAHPIVKLMLVPDTYEIGAAAWW
                     RVFEIGAWIFADATIMVIAVLQSLKTLHKYAAHSATLSLTNETIGKHVEERTRELERS
                     REQYRLIAETTRAIPFELDLALGRFTYIGPQAQKMLGFTETRWREAGFLDVLLPRSRE
                     SSVRQQLDECMPGTFEALCSVVTADDRVVELRWTVFCELVDDSRFLRGLMIDVTESRR
                     MVRELAQGQKLESVGRIAAGVAHEINTSVQFISDSVRFVRHALKDVPHALADYRALAA
                     GALSGKDVTAAAKKAADTDEAADVDYFLKNAPDALDRALEGIGRVGSIVRSMTEFAHP
                     DSRTKSDVDLNRAIRTTLNMARNEYKTVAELETDFGDIPPVHCHAGDINQVVLNLLLN
                     AAHAIGDVVEGSSAKGRIVVRTRAIGGYVEISITDTGDGIPEDVRGRIFEPFVTTKEV
                     GRGTGQGLALSRGIVVEKLKGSLHFETETGKGTTFFIRLPVADVMAGSDSTNKQAAA"
     gene            complement(2044..2853)
                     /locus_tag="VIV63_02080"
     CDS             complement(2044..2853)
                     /locus_tag="VIV63_02080"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY6123412.1"
                     /translation="MVPAQGPSLITASFNFELWLTALPVLLVAATLTWLASLPLRNVS
                     IVDSLWSLMLFAAGVVYALGADPRAPRLAFVLWPLAIWAARLALFITVRSIGKGEDRR
                     YQAIRARNQPGFAFKSLYLVFWLQAVLAWVISLPLLGAFASNTPLGLLDYFGLSLWVV
                     GFAFEAGGDWQLARFKRNPANADAVMNTGFWRYTRHPNYFGEFCIWWGFFAMAVSAGG
                     WWSLPGPALLTLLLLRVSGVALLEKDIGNRRPQYADYVLKTNAFFPGPTRK"
     gene            complement(2826..>3367)
                     /locus_tag="VIV63_02085"
     CDS             complement(2826..>3367)
                     /locus_tag="VIV63_02085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6123413.1"
                     /translation="SLARKLMSVGTYIAATDVLGEDRAARLITNNAAVADINRVLDYF
                     RRSADHRLLFGGRVSYSGVDPFDSARVLRGRIERVFPRLNPRIEYAWGGYVDITPNRA
                     PHFGRLAPNAYFLQGFSGHGMVLAGIAGKLVAEAMAGTASRFDVFAKIPHRDFPGGTY
                     FRRPALVLAMLWFRLKDLL"
BASE COUNT          648 a         1036 c         1132 g          551 t
ORIGIN      
        1 gtcatgtccc agcgcttgcg ttccttgcgc agggaatttc gcaagccatc gagaatggct
       61 tgttcgtcgt cgacaaatac cacgtgtagg gaagtcatgc ggcggcctgc ttgttggtgg
      121 aatcggaccc ggccatcaca tccgccacgg ggaggcggat gaagaaggtc gtgccctttc
      181 cagtttcagt ttcgaaatga agagaacctt tgagcttttc gaccacgatg ccgcgcgaca
      241 gcgccaggcc ttgcccggtg ccgcgcccga cttctttcgt ggtcacgaag ggttcgaaga
      301 tgcgcccgcg cacatcttcg ggaatgccgt cgccggtgtc ggtgatggag atctcgacat
      361 agccaccgat cgcccgggtg cggaccacaa tgcggccctt ggcgctgctg ccttcgacga
      421 cgtcgccgat ggcatgcgcg gcgttgagta aaaggttcaa aaccacctgg ttgatgtccc
      481 cggcgtggca gtggacgggc ggaatgtcgc cgaagtcggt ttcgagttcg gcgacggtct
      541 tgtattcgtt gcgcgccatg ttcagcgtgg tgcggatcgc gcggttgaga tcgacgtcgg
      601 acttcgtgcg cgaatcggga tgcgcgaatt cggtcatgga ccgcacgatg gagccgacgc
      661 ggccgatgcc ttcgagcgcg cgatcgagcg cgtccggcgc gttcttgagg aagtagtcga
      721 cgtccgcggc ctcgtccgtg tcggcggcct ttttcgccgc cgcggtcacg tccttgcccg
      781 agagcgcacc ggcggcgagc gcgcgatagt cggctagcgc atgcggcacg tctttgagcg
      841 cgtggcgcac gaaacgcacg ctgtcggaga tgaattgcac ggaggtgttg atctcgtgcg
      901 ccacgcccgc ggcgatgcgg cccacggact cgagcttctg accctgcgca agttcgcgca
      961 ccatgcggcg cgactcggtc acgtcgatca tcaggccgcg aaggaaacga ctgtcgtcga
     1021 ccagttcgca gaacaccgtc caacgcaact cgaccacgcg gtcgtcggcg gtcactaccg
     1081 agcacaacgc ttcgaaggtg ccgggcatac attcgtcgag ctgctgccgc acgctcgatt
     1141 cgcgtgagcg cggcagcagg acgtccagga aacccgcttc gcgccagcgc gtttcggtga
     1201 agccgagcat cttctgcgcc tgcgggccga tgtaggtgaa tcggccgagc gccaggtcga
     1261 gctcgaaggg gatggcgcgc gtggtttcgg cgatgagccg gtattgctcg cggctgcgtt
     1321 cgagttcgcg cgtgcgctct tcgacgtgct tgccgatggt ttcgttggtc agcgacagcg
     1381 tggcggagtg cgcggcgtac ttgtgaaggg ttttcaaact ctgcaatacg gcgatcacca
     1441 tgatcgtcgc gtccgcgaag atccatgcgc cgatttcgaa cacccgccac cacgccgccg
     1501 cgccaatctc ataggtgtcg ggcaccagca tgagcttgac gatgggatgc gcgatggcga
     1561 tgacggtggc tgtcaccagc acgcgccagt cgcgatagaa ggcgaggaac gccagcgaca
     1621 tgaacacgtg gagctgcgcc tcgggctggc cttcgagcag ccatatgaac agcatcgacc
     1681 agccgatctg acaggccgcg aggtaatggc gaacccagga ggcctgcggc gcggcgcgcg
     1741 cgaacagcag tcccggcacc gagagcacag cacccaggat catcgtgaag acgatgcgcg
     1801 gctcgcccgg aaacgatgac ttccaggcca gcaccacggc aacggcccac tgcaccgcga
     1861 atacgaagtg aaacaggcga tgagcagcca cggtgagagt cgccacgaca tcgtgacgca
     1921 cttcacgggc tcgagcctcg cgctgcgctg aatccacttc gatcacgggt ttgcctgctt
     1981 gatcctggtg ccgactccgg ttgtctcccg gggttatcgg cccattacag ggcacccttt
     2041 aggctatttg cgtgtaggac cggggaaaaa ggcgttcgtt ttcaagacgt agtcggcata
     2101 ttgcggccga cgattgccga tgtccttttc cagcagggcg acccccgaaa cccgcagcaa
     2161 aagcagggtc agcagcgcag gtcccggcag tgaccaccag ccgccggccg aaaccgccat
     2221 ggcgaagaac ccccaccaga tgcagaactc gccaaagtag ttagggtgcc gcgtgtagcg
     2281 ccagaacccg gtgttcatga cggcatcggc attggcgggg ttccgcttga atcgggcgag
     2341 ctgccagtcg ccaccagcct cgaaagcgaa accaaccacc cacagtgaaa gcccgaagta
     2401 gtcgagcagg ccgagcggtg tgttgctcgc gaaggcgccc agcaacggca gcgatatgac
     2461 ccaggcaagg acggcctgga gccagaacac gaggtagagg ctcttgaagg cgaaaccggg
     2521 ctgattgcgc gcgcggatgg cctggtagcg ccggtcttca cctttgccga tgcttcgcac
     2581 ggtgatgaag agcgccaggc gcgccgccca gatcgcgagc ggccacaaca cgaacgccag
     2641 gcgcggcgcg cgcgggtcgg cgcccagcgc atacacgacg ccggcggcga acaacatgag
     2701 gctccacaac gagtcgacga tggagacgtt ccgcagcggc agcgacgcga gccacgtgag
     2761 cgtggcggcg accagcagta ccggcagcgc ggtgagccac agctcgaagt tgaaactggc
     2821 cgtaatcaga gaaggtcctt gagccggaac cacaacatcg cgagtaccag ggccggccgg
     2881 cggaagtatg tgccgcccgg aaagtcgcga tgaggaatct tcgcgaacac gtcgaagcgg
     2941 gaggcggtgc cggccatcgc ttcggccacg agttttccgg cgatgcccgc cagcaccatg
     3001 ccgtgaccag agaagccctg gaggaagtag gcgttcgggg cgaggcggcc gaagtgcggc
     3061 gcgcggttcg gggtgatgtc gacgtaaccg ccccacgcat attcgatgcg cggattcagg
     3121 cgcgggaaca cgcgctcgat ccggccgcgc aggacacggg cagaatcgaa agggtcgacg
     3181 cccgagtaac tcacgcggcc gccgaacagc agccggtgat ccgcggagcg gcggaaatag
     3241 tcgagcaccc tgttgatgtc ggccacggcg gcgttgttgg tgatgagcct ggccgcgcgg
     3301 tcctcgccga gcacgtcggt ggccgcgata taggtgccga cggacatgag cttgcgcgcc
     3361 agcgacg
//
LOCUS       DATDEU010000090         6920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1620816, whole genome shotgun sequence.
ACCESSION   DATDEU010000090 DATDEU010000000
VERSION     DATDEU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620816"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2365)
                     /locus_tag="VIV63_02090"
                     /pseudo
     CDS             complement(1..2365)
                     /locus_tag="VIV63_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2618..3103
                     /locus_tag="VIV63_02095"
     CDS             2618..3103
                     /locus_tag="VIV63_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY6123414.1"
                     /translation="MADKPRARRAYKKSSIAAVGLDLDCLPTLLGFNIRRAQIALWRD
                     FNRSFSDGDVRPGVFSAMLLANANPGIAQTDIASQLGIDKASVVSLVDRMEHTGWVER
                     RRSTEDRRRQGIFLTPVGVKACRSLRKEMIEHERKFVSRYTEQELRLLISLLRRLHDA
                     G"
     gene            3188..4291
                     /locus_tag="VIV63_02100"
     CDS             3188..4291
                     /locus_tag="VIV63_02100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY6123415.1"
                     /translation="MSLDLVFAGDLVLDEPHPDHWLEGLAPVLRAADVAIGHLEVPHT
                     RRGSELRSDIPAPGADPAHVPALARAGFHAVSLAGNHIADCGAVGIDDTIAALDAAGI
                     AHSGAGANLVAARAPCVLQRGSWQIALLSYNCVGPEESWATATRAGCAYVRVDTADGS
                     RIAPAAPLVTASETSLAQLAADISRARGARTLVIVALHKGIVHTPARLAPYERAIAHA
                     AIEAGADIVLGHHAHIVRGIEIHRGKPIFHGLGNGCVVTRALSPDSDDPARAAWAVRR
                     QQLFGFTPDAAYHLAPFHPQAVNAMLGRVRVNDDGSLETGFVTVHVDAPGRPRIADDS
                     EAIAISRYVEEITATAGLPPITTRLDGACAWIR"
     gene            4279..4749
                     /locus_tag="VIV63_02105"
     CDS             4279..4749
                     /locus_tag="VIV63_02105"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY6123416.1"
                     /translation="MDPVKPQVKRGVLFYVGATGLLAVMVIEVIAVIGRHIRVPLLGA
                     LELAQAAIVPAACASMVIASLTNAHAAVHLVTERVPEHVRGAMARFSAILAGVFFLGI
                     ATGSGWLTIEFWNSFEETDVLHIPFRPLRVLVTVCAMALAFIFFHRGVSRGDRR"
     gene            4746..6035
                     /locus_tag="VIV63_02110"
     CDS             4746..6035
                     /locus_tag="VIV63_02110"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY6123417.1"
                     /translation="MIGICGVIALLALLAVGVPIGVAMGFVGLAGMCLLLGLEPALIK
                     TGVVLFESVSRYELGVLPLFLLMAHLCFVAGASRDFFAAAASVVGHRRGGLALATIAG
                     CAGFGAVSGSSLATAATMGLIGLPEMRKRGYSTALASGSVAAGGTLGSLTPPSGALIV
                     FGIIAEQSIGKLFTAAIIPALTQTLFYMATIVIVCRIRPSIAPPSPRATWAERRAAIF
                     RVLDLAALVACVLTGIAVGWFTPTEAASVGALLALGLCALRRRLTWQSLGDALAQTLR
                     TTGMLYVVIVGALIFAVFMSFTGLAEQVSALVHSLDGGPMVAIIAITILLLLLGSVLD
                     GLALMLLTTPILLPIVTDLGFSPIWFGIFLVRTMEIGFVHPPIGINLYVIQSIAPDIP
                     LSRVFKGVLPFLAADFLHLALLIAIPALTLGLPALLS"
     gene            6042..>6920
                     /locus_tag="VIV63_02115"
     CDS             6042..>6920
                     /locus_tag="VIV63_02115"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEY6123418.1"
                     /translation="MNAQVPNYLDHVQCLLVEPLLAARAAARTGTRVVGYIGNDVPVA
                     LILAANALPVRLRCDARPSTARADQFLESAFAPEMRSIFEQWLAGELAFVDSVVFPRT
                     DDSAQRLYYYLCELQRRGLCGGPRPLLYEVADIARASSSGYTRESTHRLAQALGTRED
                     LLPSAAQRIAQREALLAEIRARRAAKSPLAGSLGWRVDHANSCDWREEFDTATHHWLK
                     EAPALSGPRRILLAGDPPPDDSLHRVIEDAGGTVVLELTESVAGAMSTQSASIDAIAD
                     HFHSRRNPVREMRANGD"
BASE COUNT         1156 a         2251 c         2214 g         1299 t
ORIGIN      
        1 agcgcagcgg gttgtagatg tagttgagtt gatcgaggcg atcggccgac ggtgttatcg
       61 cgacttcata agtgtcgaag taggtcgagc tcagacccaa ctcgaagttg cccgccgcgc
      121 ccgcctcgaa ttcgtaggta cccataaaat cgaagcccga ggcgatggtc gtaccaagat
      181 tgttagtccg gccgtcaacg tacagcgtga cgggattcgg aagtacgccg cggatgggct
      241 tggtggcgac caggctcgcg atgaacgcgg gatccggatt gcgcacgatg attcccgtgc
      301 ccgcgaactg atcctcacga ttcaggatgg tcaggtcgga caggtacgag gtgatctgct
      361 tttcgtagtc gatatggaaa tacgtcagat tgaaacgcag gttcgatgtg ggcgcgaagt
      421 ccgcaccaat cgtgtaggtg ctcgcggtct cgggctcgag ctcgaggttg ccacccgaca
      481 acgtcacacc ctgcgtgatc gcgccaccgc gcgtgggatc ggagtagttc tgcacgaaga
      541 gcgcctatga gttgccatag atctgcgcga aggtcggcgc gcggaaagat tcgccgtagg
      601 tagcccggaa cttgagattt tccagcggcg cccagtcgat gccgtatttt ggattgaccg
      661 tcgagccgtc ggtgagatcg ctgtagttgt cagaacggac ggcgagatcg acgacgagct
      721 gctgcaagcc gggccgggcg ttctcggcac cgaacagcgg gatgagaact tccgcgtagg
      781 cagaattcac tgtgcgcctg acttccggtg taattgcgcg caccggcgct tcccaggttc
      841 cggtgatcaa tcccgacccg ctggtcagaa gctggccttc gtacccgatg gccgcgcgca
      901 actcgccgcc cggcagcgtc gcgagcggac cgtcgaattt gacttcccac ccttcgaaat
      961 gcgtgtcgcc cggtgcaatg aacagctggt tggccaagcc ctgcaacgcc gcggcattgt
     1021 taggcgcggt actgaacggg ttgagtgccg tggccggatc tgtacgcgcc agtgccgccg
     1081 tcagcgcggc gttgttgatg ccgtactgcg aagtgctgcg ttccgtatcg cggccgtagc
     1141 tgtaagacgc ttcgaggttc cagtcgccga atacattgaa gcccgcgccc acggtcgcct
     1201 ggtatgcctc ggagaacccc accgtatcgt tcgacggaag ttgatcgccg aacgagtagt
     1261 tgaccgtcag cgaggtggca gtgctgccag gaggcgcgac gaaataggga ttgacgttgc
     1321 ggacggtcag cgcggcggcc tggttgccca cctcgttctc gaattccctg cgcgaccaga
     1381 acgcgtcgcc gaacaccttg aagcgatcgt tgaattcgtg attgaacgtg gtgacgaatg
     1441 aattccgcac ctgctccggc agcaagtcct ggaactgggc gttgtcgcag cgattggtcg
     1501 tgccgggaac gagctgactc gcggtcgcgg ttgtcacccc tccggctgga atggcatgcg
     1561 acacgccggc gaccacgatg ttgcccgggt tgcattgcac gacccgaaaa tcccggccgc
     1621 cacgcgaagt gaggtcaccg cggaagaatt cgcgatccag cccattcagg ttgctgtggg
     1681 acgagtactc gtaggcagcc atgaggtttc cgccggtcca gccgaagccg ccgaccagcg
     1741 agccctgacg ttcgtcgtat ccatcgccgg aactgccgcg cacgctggct tcgacacctt
     1801 cgaagtcgcg ccgcaggatg aggttcacga cgccggcgac agcgtcggag ccatacaacg
     1861 ccgaggcgcc atcagccact acttcgacgc gcgcgagtgc tgcagcggga atgatcgagg
     1921 catcgacgcc gaagccggtg gtgccttgag ggacggcgcg atgtccttcg atcagcgtca
     1981 acgtcgagaa aggtccgagt ccgcgaagat tgatggccga gccataggtg atgttgccgg
     2041 atccgcccga ttgaccgcgc gaactctccg agacgccgag attgaagacc tgcggcaatc
     2101 gctggaccag cgcggtcgtg gaaacaggtg cggcaagttc gatgtcctgg cgggaaatgg
     2161 cgatgacagc cgagcccacc ggctcggcgc cgcgaatgcg gctaccggtg acgatgactt
     2221 catcgagatt gtcggcgacg gccggcgatg tcggctcctg cgcgtgtacc gcgctggtcc
     2281 agccgcaggc cgcggcggta gcaagagcgg cgcggaccgc gcacctgact gcggtacgac
     2341 cgcgaaatgc agcaacgtga ttcataagcc ccctcgactt tgcgcaccgg aatttttcga
     2401 tcctgccgtc actctcggca gcccgtggtg ttcgcaatca gagtgcgccg gccgcgccac
     2461 gattgtcaat atggttggca ttgccaacca tttgtccatt tacaatcagc tcccgagcga
     2521 acgctggggc cggcgggtaa cggcccgttc cggacgtcgt tgaccactgc ctgcgcattg
     2581 cgtacgttcg cctccccgac gccgaaaccc accttccatg gcagacaaac cccgcgccag
     2641 gcgcgcctat aaaaaaagct ccatcgccgc ggtaggcctg gacctggact gcctgcccac
     2701 gctgctgggg ttcaacatcc gacgcgcgca gatagccctc tggcgcgact tcaatcgcag
     2761 cttctccgat ggcgacgtac ggcccggcgt tttcagcgcg atgctgctgg ccaatgcgaa
     2821 cccgggaatc gcgcagaccg acatcgccag ccagctgggc atcgacaaag ccagcgtggt
     2881 ttcgctggta gaccgcatgg aacacaccgg ctgggtcgaa cggcgccgct cgaccgaaga
     2941 ccgccgccgt cagggcatat tcctcactcc cgtgggcgtg aaggcgtgcc gctcgctgcg
     3001 caaggaaatg atcgagcacg agcgcaagtt cgtgagccgt tacaccgagc aggaattgcg
     3061 cctgctcatc tcgctgctgc gcaggttgca cgacgccggc tgaccgccca ccgccggcta
     3121 ccactatcga ctccgacccg gattcggtgg taaatgtgaa ttagttgaac ttagaaacta
     3181 atttcgaatg agcctcgatc tggtattcgc tggcgatctc gtgctcgacg agccgcaccc
     3241 cgaccattgg ctcgaggggc tggcacccgt gcttcgcgcg gccgacgtgg cgatcgggca
     3301 tctcgaagtt ccacatacgc ggcgtgggtc cgagctacgc agcgacatac ccgcgcccgg
     3361 cgccgatccg gcccacgtgc cagcgttggc ccgcgccggc tttcacgcgg tctctctcgc
     3421 gggaaatcac atcgccgatt gcggcgccgt gggtatcgac gacacgatcg ccgcactcga
     3481 cgccgcgggc atcgcgcaca gtggcgccgg cgccaacctc gtcgccgcgc gcgctccctg
     3541 tgtgctgcaa cgaggttcgt ggcaaatcgc cctgctcagt tacaactgcg tgggcccgga
     3601 agaatcctgg gcgacggcga cgcgcgcggg ctgcgcctac gtgcgcgtcg acacggcgga
     3661 tggctcgcgt atcgcgcccg cggcgccact ggtgaccgcc agcgagacgt cgctcgcgca
     3721 gctcgcagcc gacatttctc gcgcgcgcgg tgcgcgaacg ctggtcatcg tcgcgctcca
     3781 caagggcatc gtgcacacac ccgcgcgcct cgcgccttat gaacgcgcca tcgcgcatgc
     3841 cgccatcgag gcgggagcgg atatcgtgct cggccatcac gcacacatcg tccgcggcat
     3901 cgagatccac cgcggcaaac cgattttcca tggcctcggc aacggctgcg tggtgacgcg
     3961 cgccctgagc ccggacagtg acgatccggc gcgtgcggcg tgggcggtcc ggcgccagca
     4021 gttgtttgga ttcacgcccg acgcggccta tcacctcgcg ccgtttcatc cgcaggccgt
     4081 gaacgccatg ctcggacgcg tgcgcgtgaa cgatgacggc agcctcgaga cgggtttcgt
     4141 caccgtgcat gtcgacgcgc cggggcgccc gcgaatcgcc gatgattccg aagcgatcgc
     4201 gatctcccgc tacgtcgagg aaatcaccgc gacagcgggg cttccgccca tcacgacgcg
     4261 gctggatggg gcgtgtgcat ggatccggta aagccgcagg tgaagcgcgg cgtgctgttc
     4321 tacgtcggcg cgacgggact gctcgcggtc atggtgatcg aagtgattgc ggtcatcggc
     4381 cggcatatcc gtgtgcctct actcggcgcg cttgaactcg cgcaggccgc catcgttccg
     4441 gcggcgtgtg cgtcgatggt gatcgcctcg ctgacgaatg cacatgcggc ggtgcacctg
     4501 gtcaccgaac gcgtgcccga acacgtgcgc ggcgccatgg cgcgcttcag cgcgattctc
     4561 gcgggtgtgt ttttcctggg aatcgccacc ggttctggct ggctgacgat cgaattctgg
     4621 aattccttcg aagagacgga cgtcctccac attccgtttc gacctctgcg agtgctcgtc
     4681 acggtttgcg ccatggccct cgcgttcatc ttcttccacc gcggcgtgag ccgtggagac
     4741 cggcggtgat cggtatttgc ggcgtcatcg cgctgctcgc gctgctggcg gtgggcgtgc
     4801 cgattggtgt cgccatggga ttcgtgggtc tggcgggcat gtgcctgctg ctgggtctcg
     4861 aacccgcgct catcaaaacc ggcgtggttc tcttcgaatc ggtgtcgcgc tacgagctcg
     4921 gtgtgctgcc gctttttctg ctgatggcgc acctgtgttt cgtggcaggc gccagccgcg
     4981 actttttcgc cgcggccgcc agtgtcgtcg ggcaccggcg cggtggcctc gcgctcgcca
     5041 ccatcgcggg gtgcgcgggc ttcggcgcgg tcagcggctc gagcctggca accgccgcga
     5101 ccatgggact gatcggcctg ccggaaatgc gcaagcgcgg ttactcgacc gcgttggcat
     5161 ccggttcagt ggccgccggt ggcacgctcg gctcgctgac tccgccgtcc ggcgcactca
     5221 tcgtcttcgg catcatcgcc gagcagtcga tcggcaaact attcactgcg gcgatcattc
     5281 ccgcgctgac gcagacactg ttttacatgg cgacgatcgt gatcgtctgc cgcatccggc
     5341 cttccatcgc gccgccgtca ccacgcgcaa cctgggcgga acgacgagct gccattttcc
     5401 gcgtactcga tctcgccgcg ctggtcgcgt gcgtactcac gggcatcgcg gtgggctggt
     5461 tcacgccgac ggaggcggcg tcggtgggcg cactgctcgc gctcgggctt tgcgcattgc
     5521 gccggcggct cacctggcaa tcactcggtg acgcgctggc gcagactctg cgcaccacgg
     5581 gcatgttgta cgtcgtcatc gtcggcgcac tgatcttcgc ggtgttcatg agcttcacgg
     5641 gactggccga acaggtgtcg gccctggttc actcgctgga tggtggcccg atggtggcga
     5701 tcatcgccat cacgatactg ctgctcctgc tgggctcggt tctcgatgga ctcgcgctga
     5761 tgcttctgac cacgcccatt ctcctgccca tcgtcaccga tctgggtttc tcgcccatct
     5821 ggttcgggat cttcctcgtg cgcaccatgg aaataggatt cgtccatcca cccatcggca
     5881 taaacttgta cgtcatccag agcatcgcgc ccgatattcc actcagccgc gtgttcaagg
     5941 gagttctgcc gttcctggcg gccgatttcc tgcatctggc gctgctgatc gccatccccg
     6001 cgctgacgct gggcctgccc gccctgctga gctgaccgcc gatgaacgcg caagtcccca
     6061 actacctcga tcacgtgcag tgcctgctcg tcgaaccgct gctcgcggcg cgcgccgccg
     6121 cacgcaccgg aacgcgcgtg gtcggctaca tcggcaatga cgtgccggtc gcgctgatcc
     6181 tcgccgcgaa tgcgttgccc gtaaggctgc gttgcgacgc gcggccttcc acggcacgag
     6241 cggatcagtt tctcgaatca gcgttcgcgc ccgagatgcg ctcgatattc gaacagtggc
     6301 tcgcgggcga gctcgccttc gtggactccg tggtttttcc gcgaacggac gactccgcgc
     6361 agcggctcta ctactacctt tgcgagcttc agcgccgcgg tctttgcggc ggaccgcgac
     6421 ccttgctcta cgaagtcgcg gatatcgcgc gggcttcgag cagcggatat acacgcgagt
     6481 cgacacatcg tctcgcacag gccctcggca cccgcgaaga cctgttgccg tcggccgcgc
     6541 agcggatcgc acagcgcgaa gcgttgctgg ctgaaatccg cgcgcgtcgc gctgcgaagt
     6601 ctccgctggc aggcagcctg ggctggagag tcgaccacgc gaattcgtgc gactggcgcg
     6661 aagaattcga tacggcgacg catcactggt tgaaggaagc gcccgcgctt tccggacctc
     6721 gccgcatcct gctcgccgga gatcctccgc cagacgattc actgcatcga gtcatcgaag
     6781 acgcgggtgg aacggtggtg ctcgaattga ccgaatccgt cgcgggcgcg atgtccacgc
     6841 agtccgcgtc catcgacgcc atcgccgatc acttccactc gcgacgaaat ccggtgcgcg
     6901 agatgcgcgc caacggcgac
//
LOCUS       DATDEU010000091         3375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1912861, whole genome shotgun sequence.
ACCESSION   DATDEU010000091 DATDEU010000000
VERSION     DATDEU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912861"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..712)
                     /locus_tag="VIV63_02120"
     CDS             complement(188..712)
                     /locus_tag="VIV63_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY6123419.1"
                     /translation="MHLTSTMQRYIVHWGEMGSRWGMNRSVAQIHALLYLSEKPLNAD
                     EIGETLGIARSNVSTGLKELASWELVQVTHVLGDRRDFFTAQHDTWEILRVIIEGRKR
                     RELDPTLAALRECAAQLKGDKETPEAVRDRIVTQLEFLETVTRWYDSVKRLPRKTLLK
                     MMRMGERIAKVIGD"
     gene            804..1757
                     /locus_tag="VIV63_02125"
     CDS             804..1757
                     /locus_tag="VIV63_02125"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY6123420.1"
                     /translation="MSGAQRESLPLPASLATHRVRHRDASDVVEGTVAFHPVKGLWFL
                     GMFAGAVAGAVFFFSVSALLLFFVSTAIVLLFGHSLGSHRKLVHDSYQCPRWLEYTLV
                     YLGVQVGLAGPIGLLRQHEMRDFAQRLPVCHDYLRHGRPFWIDAWWQLCCELRLEHAP
                     RIAVEERVTGNRFYRWLERTWMAQQIPWAILFYVWGGWAFVCWGVCARVTAGVFGHWI
                     IGYLAHNHGEMERVVVGAAVQGRNVRFTSLLTMGECWHNNHHAFPGSARLGLRPGEWD
                     PGWWVLSGLEHLGLVWAIRLPADLPPRAEVRPALVSSAHLA"
     gene            1754..2749
                     /locus_tag="VIV63_02130"
     CDS             1754..2749
                     /locus_tag="VIV63_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123421.1"
                     /translation="MSDPEDKLPYRGWWPFAGGVLVGLVLRLIFIGDPGEAYAAMMDS
                     FIIGAPALVAVVTVYIAELKSRRSWSYYFVAPALANVLYVLGTLLILVEGWICAIIIF
                     PLFALVGGLAGLAMGAICRITHWPRRAVVGCVALLPLITGGLEQRLELPVRERVTERE
                     ILVDADAAGVWRELVDARDIAPAELDDAWLYRIGVPLPVAAAADTRDGAHLRHMTMGK
                     GIHFDQVATEWRENEHVTWRNHFAPDSFPAGALDDHVRIGGHYFDVAETQYTLSESGG
                     QTLLRIRMSYRVSTQFNWYAGPVADVLVGNFADVVLGFYARRAEKAANLQAVAPS"
     gene            complement(2728..3195)
                     /gene="greB"
                     /locus_tag="VIV63_02135"
     CDS             complement(2728..3195)
                     /gene="greB"
                     /locus_tag="VIV63_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY6123422.1"
                     /translation="MTAAGAARLKQELDELWKTERPRVTQAVSEAAAQGDRSENAEYT
                     YGKKRLREIDRRVRFLRKRLDGMTVVDSVPADRKRVFFGAWVTLEDDDGKQTKYRIVG
                     PDEFDMADGYISMDAPLARALMRKALDDEIKVALPGGERAYVIVDIRYEGATA"
BASE COUNT          489 a         1071 c         1095 g          720 t
ORIGIN      
        1 cgatctcgaa ggcgcccgca taagtcaccg ggtcggtgga ttcggcgaag cgctcgcgca
       61 ccgcttccgg aagccggctc cagccttcct taccaatgac ttcacgcagc ccgtgacgat
      121 ggccgtgagc tacgcgttcg agcgtcagtg gagtcgtcag tgtggtggcc gcgttcatgg
      181 tgtctcctca gtcgccgatg accttggcga tgcgttcacc catgcgcatc atcttgagca
      241 gcgtcttgcg cggcagccgc ttcacggagt cgtaccagcg cgtgacggtt tcgaggaact
      301 cgagctgcgt cacgatgcgg tcgcgcacgg cttcgggcgt ctccttgtcg cccttgagct
      361 gcgccgcgca ttcccgcagc gcagcgaggg tgggatcgag ctcgcggcgt tttcggccct
      421 cgatgatcac gcggaggatt tcccaggtgt cgtgctgcgc ggtgaagaaa tcgcgccggt
      481 cgccgagcac gtgcgtgacc tgcaccagct cccaggaggc gagttccttg agcccggtgc
      541 tgacgttgga gcgggcgatg ccgagcgtct cgccgatttc gtcggcattg agcggcttct
      601 cggagagata gagcagcgcg tggatctgcg cgacggagcg gttcatgccc cagcggctgc
      661 ccatttcgcc ccagtggact atgtaacgct gcatggtgct ggtgaggtgc atatcgtctt
      721 gcctgttatt tctgtcttga cagaaattat aggccgaaca atcatggccg caagcgggca
      781 atgacatatt tacggggaat ttcatgagcg gagcacagcg agagtcgctt cccctgccgg
      841 cgagcctggc cacgcatcgc gtgcggcatc gcgacgccag cgacgtggtc gagggcacgg
      901 tcgcctttca cccggtcaag ggcctctggt tcctgggcat gttcgccggt gccgtcgccg
      961 gcgcggtctt cttcttcagc gtgtccgcgt tattgctgtt cttcgtcagc accgcgatcg
     1021 tgctgctctt cgggcattcg ctcggcagtc atcgcaagct ggtgcacgac agctaccaat
     1081 gtccgcgctg gctcgaatac acgttggttt atctgggcgt gcaggtcggt ctcgcgggac
     1141 cgatcggcct tttgcgccag cacgagatgc gcgacttcgc gcaacggctt cccgtctgtc
     1201 acgactatct gcgtcacggc cgcccgttct ggatcgacgc ctggtggcag ttgtgctgcg
     1261 agttgcggct cgagcatgcg ccgcgcatcg cggtcgaaga gcgcgtcacc ggcaatcgtt
     1321 tttaccgctg gctggagcgc acgtggatgg cgcaacagat tccctgggcg attcttttct
     1381 atgtgtgggg cggctgggcg ttcgtctgct ggggcgtttg cgcgcgcgtc accgccggcg
     1441 tgttcggaca ctggatcatc gggtatctcg cgcacaacca cggcgagatg gagcgtgtcg
     1501 tggtgggcgc cgccgtgcag ggccgcaacg tgcgcttcac ctcgctgctc accatgggtg
     1561 agtgctggca taacaatcac cacgcgtttc ccggctccgc gcgtctcggg ttgcgccccg
     1621 gtgaatggga tccgggctgg tgggtgttgt ctggtctcga gcatctcggc ctggtctggg
     1681 cgatccgcct gcccgccgac cttccgccgc gcgctgaagt ccgccctgcg ctcgtatcct
     1741 cggcgcacct cgcatgagtg accccgaaga caaactgccg taccgcggct ggtggccgtt
     1801 cgccggtggc gtcctggttg gactcgtgct gcgcctgatc ttcatcggcg atcccggcga
     1861 ggcgtacgcg gcgatgatgg attccttcat catcggcgcg ccggcattgg tcgctgtcgt
     1921 cacggtgtac atcgctgaac tcaaatcccg gcgaagctgg tcttactact tcgtcgcgcc
     1981 cgcgctcgcg aatgtgctgt acgtgctcgg aacgctgctg atcctggtcg aaggctggat
     2041 ttgcgccatc atcatcttcc cgttgttcgc cctggtgggc ggcctcgcag gtctcgccat
     2101 gggtgcgatc tgccgaatca cccactggcc gcgccgcgcc gtcgtcggtt gcgtggcgct
     2161 gttgccgttg atcaccggag gactcgagca acgcctcgag ctaccggtgc gtgagcgcgt
     2221 cacggagcgc gaaatcctcg tcgatgcgga tgcggccggc gtatggcgcg aactcgtcga
     2281 cgcgcgcgac attgcgcccg cggaactcga tgacgcatgg ctttaccgca tcggggtgcc
     2341 gctgccggtg gccgccgccg cggatacgcg cgatggcgcc catctgcggc acatgacgat
     2401 ggggaagggc atccacttcg accaggtcgc gaccgaatgg cgcgagaacg agcacgtcac
     2461 ctggcgcaac cacttcgcgc ccgactcatt cccggccggc gcgctcgacg accacgtgcg
     2521 tatcggcggt cactatttcg atgtcgccga aacgcagtac accttgagcg agagcggcgg
     2581 ccagaccctg ctgcggatcc gcatgagtta tcgcgtgagc acgcagttca actggtacgc
     2641 cggcccggtc gccgacgtgc tggtcggcaa tttcgccgac gtcgtgctgg gtttctatgc
     2701 gcggcgcgcc gaaaaggcgg ccaatcttca ggctgtcgca ccctcgtagc ggatgtcgac
     2761 gatcacatag gcgcgctcgc cacccggcag cgcgaccttg atctcgtcat ccagcgcctt
     2821 gcgcatgagc gcgcgcgcca gcggagcatc catgctgatg tagccgtccg ccatgtcgaa
     2881 ttcgtcgggt ccgacgatcc ggtactttgt ttgtttgccg tcgtcgtcct cgagcgtgac
     2941 ccaggcgccg aagaacacgc gcttgcggtc cgcaggaacg ctgtcgacca ccgtcatgcc
     3001 gtcgaggcgt ttgcgcagaa aacgcacgcg ccggtcgatt tcgcgcaacc gtttctttcc
     3061 ataggtgtat tcggcgtttt ccgaccggtc gccttgcgcc gccgcttcgg acaccgcctg
     3121 ggtgacgcgc ggacgctcgg ttttccagag ttcatccagc tcttgtttga gccgtgctgc
     3181 acccgcggcg gtgatgaatt gcgatccggg tttctctgtc ggtcggtatc ggctcacgtc
     3241 ttttcaatca tgtgtgatgg gtgtctcaat cgcggcggca gtctgtccgg gggttctgtg
     3301 cagcggctca cagccgtttg ggggtggtca atttacgctt tctacccatg gcagcccgtt
     3361 actacacgca ctttg
//
LOCUS       DATDEU010000092         3571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1038257, whole genome shotgun sequence.
ACCESSION   DATDEU010000092 DATDEU010000000
VERSION     DATDEU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038257"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VIV63_02140"
     CDS             <1..340
                     /locus_tag="VIV63_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6123423.1"
                     /translation="ADAPKTAGELEKGGVLFAFASAGLKEPVDFVKNAARAVKAGLAP
                     DAAVRALTINAAKIAGVADRLGSLEAGKVANVIVTDGDLFEDKTSVKHVFIDGRLVKL
                     VKVDAPTDQN"
     gene            794..2701
                     /gene="htpG"
                     /locus_tag="VIV63_02145"
     CDS             794..2701
                     /gene="htpG"
                     /locus_tag="VIV63_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY6123424.1"
                     /translation="MFQAEVKQILHLMIHSLYGNKEIFLRELVSNASDAADKLRFEGI
                     ANAALFEDEPDVTIRVSVDASARTITVSDNGIGMTRQEVIDQIGTIAKSGTREFVQRL
                     TADQANGASLIGQFGVGFYSAFIVADRVTVLTRKAGAAAAEGVKWESSGEGDYSVETI
                     EKPSRGTEVILHLRPEEDELLSSARVREILRRYSDHITVPILMKKESWDADAKQTVLT
                     AEDEQINQASALWARPKSEITDAQYDEFYKHIAHDVEPPLARVHARVEGRQEYTQLFF
                     IPARAPFDLWDRDQRHGLKLYVRRVFIMDDAEDLMPTYLRFVRGVIDSSDLPLNVSRE
                     TLQQSRDVQSIKTASTKRVLGLLDELATKEPEKYATFWTTFGRVLKEGLAEDAANRER
                     IAPLLRFASTHDGSEAQTVSLADYAARMRGGQEAIYYITADSFAAARNSPHLEIFRKR
                     GVEVLLMYDRIDEWVVSSVTEFEGKPLHSVAKGDLDLGKLGGDLPNDESATAGGDESP
                     LLERMQAALKDRASAVRTTERLTESPACLVSEQYGLSMNLERILKAAGQNVPASRPVL
                     EINAGHPIVKRLKSEADDARFGDWSHILFDQALLAEGGQLEDPAGFVKRLNELMLTLA
                     GEGPSRIWTPS"
     gene            complement(2888..>3571)
                     /locus_tag="VIV63_02150"
     CDS             complement(2888..>3571)
                     /locus_tag="VIV63_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123425.1"
                     /translation="RRGADGKIERADSYENELFYGTANLSISAHQLARWGAEWWKPTL
                     KPLTVTATTPASIAGNASGLSWGNWYCAPGGGRCHYVGHHEGFHHLLYWDGDRRISVA
                     MVTNNTLAPTLQQRLQRALVAFAQRSDAAAKRELSMLLPDNAVRPATYRFPTGETVTV
                     IDRENGMAVIRGGISYLAYRIGSGIRYIPGLDVYVAGTENGDLYWLSLYADMTGVPVP
                     PSAAGSPIH"
BASE COUNT          676 a         1133 c         1154 g          608 t
ORIGIN      
        1 cgccgatgcg cctaagactg ccggtgagct cgagaagggc ggggtgttgt tcgcgttcgc
       61 ttccgccgga ctcaaggagc cggtcgactt cgtcaagaac gccgcgcggg cggtgaaggc
      121 gggcctggcg cccgacgccg cggtgcgcgc cctgaccatc aacgccgcga agatcgcggg
      181 cgttgccgat cgcctgggct cgctcgaggc cggcaaggtc gccaacgtga tcgtcaccga
      241 tggagatctg ttcgaggaca agaccagcgt gaagcacgtg ttcatcgacg gccggctcgt
      301 caagctggtg aaggtcgacg cgccaacgga ccaaaattag tggtcgggat cacggggtgg
      361 ctcttgtgcc actccgtcgc ttgttcgtag gcatacgcga tgcgacacag ggtcgattcg
      421 gaccaccgtc gtcccgtgaa ttgaatgctg tacggaaggc ggtccttcgt gaaccctgag
      481 ggcaggcaaa tcgacggcac gccggccagg ttcatcggca ccgtgaactc caccgcacgc
      541 gacgattgcg aggccccacg cggcatttac gtccggcgag tttccttctc tgccgtgacg
      601 gctcaatcac agccatcgcg ttgaaatcct aggcctcggc ggcgggccaa tcgtggcgaa
      661 caaatccgac gaacgtcatg accacggcgg tgaccggcaa cagcgcgcgg ccgaaggatc
      721 cgagtagctc gccgtcgctg gtcgctccgg gtaaaatgac ggaatggatg ccactgcatc
      781 acgccaaacc ctagtgttcc aggcggaggt caaacagatc ctccacctga tgatccattc
      841 gctctacggc aacaaagaga tcttcctgcg cgagctcgtg tccaatgcct cggacgcggc
      901 ggacaagctg cgcttcgagg gcatcgccaa cgccgccctc ttcgaagacg agcccgacgt
      961 gaccatccgc gtcagtgtcg atgcctctgc gcggacgatc acggtgtccg acaacggcat
     1021 cgggatgacg cggcaggaag tgatcgatca gatcggcacc atcgcgaagt ccggcacgcg
     1081 cgagttcgtc cagcggctca ccgccgacca agccaacggc gccagcctga tcgggcagtt
     1141 cggcgtcgga ttttattccg cgttcatcgt ggccgatcgc gtcaccgtgt tgacgcggaa
     1201 ggccggcgca gccgccgctg agggcgtgaa gtgggaaagc agcggcgaag gcgactacag
     1261 cgtcgagact atcgagaagc catcgcgcgg caccgaagtg atcctgcact tgcggccgga
     1321 agaagatgag ctgctgtcga gcgcgcgggt gcgtgagatc ctgcgtcgat attcggacca
     1381 catcaccgtg ccgattctca tgaagaaaga gtcctgggat gcggacgcga agcaaacggt
     1441 gctcaccgcc gaggacgagc aaatcaacca ggcgtcggcg ttgtgggcca ggcccaagtc
     1501 ggagatcacg gacgcgcagt acgacgagtt ctacaagcac atcgcgcacg acgttgagcc
     1561 gccgctggcg cgtgtgcacg ccagggttga aggccgccag gagtacacgc agctgttctt
     1621 cattccggcg cgggcgccat tcgacctgtg ggaccgcgac cagcgccatg gactcaagct
     1681 ctacgtccgc cgcgtcttca tcatggacga tgccgaagac ctgatgccga cgtacctgcg
     1741 gttcgttcgc ggcgtgattg attcgtcgga cctgccgctc aacgtctcgc gcgaaacact
     1801 gcaacagtcg cgcgacgttc agagcatcaa gaccgcatcg accaagcgcg tgctcggcct
     1861 gctcgatgag ctcgccacca aggagcccga aaaatacgcc accttctgga ccaccttcgg
     1921 ccgcgtgctg aaggaaggcc ttgccgaaga cgccgcgaac cgcgaacgca ttgcgccgct
     1981 gctgcgcttt gcgtcgacgc acgatggcag cgaggcccag accgtatccc ttgccgacta
     2041 tgccgcccgg atgcgcggcg gacaggaggc aatctactac atcaccgcgg acagctttgc
     2101 cgccgcccgg aacagcccgc acctcgagat ctttcgcaaa cgcggcgtcg aggtgctgct
     2161 gatgtacgac cgtatcgacg agtgggttgt atcgtcggtg accgagttcg agggcaagcc
     2221 gctgcattct gtcgccaagg gcgatctcga tctcggcaag cttggcggcg acttgcccaa
     2281 cgatgagtca gcaacggccg gcggggatga atcaccattg ctcgaacgaa tgcaggcggc
     2341 gctgaaggac cgcgccagcg cggtgcgcac gacagagcgt ctgaccgaat cgcccgcgtg
     2401 tctggtcagc gagcaatacg gcctcagcat gaacctcgag cggatcctca aagctgcggg
     2461 tcagaatgtg cctgccagcc ggccggtgct cgagattaac gcgggccacc cgatcgtcaa
     2521 gcggctcaag agcgaggcag atgacgcgcg cttcggcgac tggagccaca tcctgttcga
     2581 ccaggcgctg ctggcggagg gcggacagct ggaggatccg gcaggcttcg tcaagcggct
     2641 gaacgagctg atgttgacgc tggctggcga aggaccgtcc aggatctgga cgccttcgta
     2701 agctccgcgt cgtcggccga cccgcgaggc gcgctcgcgc gattccgcca gtcgtcgaaa
     2761 cgcgcaccga gtttttggac ggcggcgcgt gcgcgttgat tgcgcagagc gatgcgcgcc
     2821 tcgagtcgct cgacgtgcat cgtatctgaa cgtcgaccca tctcggcgcg tttcaatagc
     2881 ggcgtggtca gtgaattggg ctcccggcag cggatggagg cacggggact ccagtcatat
     2941 ccgcgtacag gctgagccag taaagatcgc cgttctctgt ccccgcgacg tagacgtcga
     3001 gaccgggtat gtagcggatc cccgagccga tcctgtaggc gaggtagctg atgccgcccc
     3061 gaatcacggc catgccgttc tccctgtcaa tcaccgtcac cgtctcacct gtcggaaacc
     3121 ggtacgtggc tggccgcacc gcgttgtccg ggagaagcat cgacaattcc cgtttcgcag
     3181 ccgcgtcgct ccgctgggcg aatgcgacga gcgccctctg gagccgctgc tggagggtcg
     3241 gcgccagcgt gttgttggtg accatcgcca ccgagatcct tcggtcgccg tcccagtaga
     3301 gcaggtgatg gaagccctcg tgatgcccaa cgtaatggca tcggccacca cctggcgcgc
     3361 agtaccaatt gccccaggag agccctgacg cgttgccggc gatggacgcc ggcgtggtcg
     3421 ccgtgacggt caatggttta agcgtcggct tccaccattc ggcgccccag cgggcgagtt
     3481 gatgcgccga tatgctgaga ttcgccgtgc cataaaagag ctcgttctca tagctgtcgg
     3541 cgcgctcgat cttaccgtcc gcgccgcgcc g
//
LOCUS       DATDEU010000093         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_746462, whole genome shotgun sequence.
ACCESSION   DATDEU010000093 DATDEU010000000
VERSION     DATDEU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746462"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1019)
                     /locus_tag="VIV63_02155"
     CDS             complement(114..1019)
                     /locus_tag="VIV63_02155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY6123426.1"
                     /translation="MAFLRSVDLYPAEPARGWIPWGALAPFLALIFVIATALPGDALI
                     SKFVNISREGNPLDPTALVAFTLVPFGFLLLLLLAWVRFVERRSLESIGIRGEHKLRE
                     FLRGHAFGLSGLFAIVMVIWMAGGLQLTAVAPAFSQPASLAPIVLLLFTFALQSSVEE
                     LLFRGWLMSVLSKKFNVPIAVVVSSALFSLLHFSPRAHWLVSVGTFLFGLFACAWVLR
                     TRSVLGIMGWHAGWNWLLAIGFGLPLTGLDVGIPALLLDLEPAGANWLTGGPQGPEAS
                     VVCFAYFVLGIVLIRFQPGKASIAS"
     gene            complement(1036..>2134)
                     /locus_tag="VIV63_02160"
     CDS             complement(1036..>2134)
                     /locus_tag="VIV63_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY6123427.1"
                     /translation="RAVDYRGAGTVEFIATGSDFYFMEMNTRLQVEHPVTEMITGLDL
                     VEWQLRVASGEPLPRTQAELAIAGHAIEARVYAEDPMRDFLPSIGKLAHLVTPPASAD
                     VRIDTGVREGDEITPYYDPMIAKLIVHGATRAEAIGRMRAALAQYQVVGVRTNVEFLG
                     RLMSAPAFVEGRLDTALIERERGHLFAPRAETTPSMWDRAAQALWRAQRGDRRAAAHV
                     SPWDDRGGWALGVRGERRWKLREGDVEREVAARPSNDETRGAFVLDREVHLFLDGEHR
                     VFQWANPYLPETESAEAHGGLLAIMPGRVLAVHVKAGDEVAKGAPLVVLEAMKMEQTI
                     TAPAAGKIARVLCAPGDQVREGAELLAFEDP"
BASE COUNT          410 a          748 c          663 g          313 t
ORIGIN      
        1 gacacggaag tgaagaagtc gctgggcttc gccgccgacg atcacctcgt cgggttcgtc
       61 tataccggcc gcggcacggg cgcgaccctg gcgacgggca aatccgccac ggttcaggac
      121 gcgatgctcg ccttccccgg ctgaaaccga atcagcacga tgcccagcac gaagtaggcg
      181 aaacacacga cgctggcttc ggggccctgc ggcccacccg tgagccagtt cgcgcccgcg
      241 ggctcaaggt ctaacaacaa cgccgggatc cccacgtcga gaccggtgag tggcagcccg
      301 aatccgatgg ccagcagcca gttccagccg gcatgccagc ccatgatgcc gagcacgctg
      361 cgcgttctca gcacccacgc gcaggcaaac aacccgaaca ggaacgtgcc gacggacacc
      421 agccagtgcg cacgcgggct gaaatgcagc agcgagaaca atgccgagct cacgacgacg
      481 gcgatgggga cgttgaattt cttcgacagc accgacatca gccagccgcg gaacagcagc
      541 tcttcgacgc tcgactgcaa cgcgaacgtg aacagcaaga gcacgatcgg cgccagcgag
      601 gccggttgcg agaaggccgg cgccacggcc gtgagctgca aaccgcccgc catccagatg
      661 accatgacga tggcgaagag ccccgaaagt ccgaacgcgt gaccgcgcag gaattcgcgc
      721 agcttgtgct cgccgcgaat gccgatgctc tcgagagacc ggcgctcgac gaacctgacc
      781 cacgccagca gcaacagcag caggaagccg aagggcacca gcgtgaacgc caccagcgcc
      841 gtgggatcca gtggatttcc ctcgcggctg atgttcacga acttcgagat cagcgcgtcg
      901 ccgggcagcg cggtggcgat gacgaagatg agcgccagaa acggcgcgag cgcgccccag
      961 ggaatccagc cacgcgccgg ctccgccgga tacaggtcca ccgaacgcag gaatgccatc
     1021 ccagtccctt tgtctttatg ggtcttcgaa cgccagcaac tccgcgcctt cgcggacctg
     1081 atcgccggga gcacacaata cgcgggcaat cttacctgca gccggcgcgg tgatcgtctg
     1141 ttccatcttc atggcttcga gaaccacgag cggcgcgccc ttggcgactt cgtcgcccgc
     1201 cttcacgtgc accgccagca ccctgcccgg catgatcgcg agcagaccgc cgtgcgcctc
     1261 cgcggattcc gtctccggta gatagggatt ggcccactgg aatacgcgat gctcgccgtc
     1321 gaggaacagg tgaacttcgc ggtcgagcac gaatgctccg cgggtttcgt cgttcgatgg
     1381 ccttgccgcg acctcccgct cgacatcacc ttcgcgcagt ttccaacggc gctcgccgcg
     1441 tacacccagc gcccagccgc cgcggtcgtc ccacggagaa acgtgcgcgg ccgcgcgcct
     1501 atcgccacgt tgcgcgcgcc acagtgcctg cgccgcccga tcccacatcg aaggcgtggt
     1561 ttccgcacgc ggcgcgaaca gatggccgcg ctcacgctcg atgagagccg tgtcgaggcg
     1621 gccctcgacg aacgccggcg cgctcatgag acgcccgagg aattcgacat tcgtgcgcac
     1681 gccgacgacc tggtactgcg cgagtgccgc gcgcatccgg ccaatggcct cggctcgtgt
     1741 ggcgccgtgc acgatgagct tggcgatcat cgggtcgtaa taaggcgtga tctcatcgcc
     1801 ctcacgcaca ccggtgtcga tgcgcacgtc ggcgctggcc ggcggcgtca ccaggtgcgc
     1861 cagttttccg atcgacggca ggaaatcacg catgggatct tcggcgtaga cacgcgcttc
     1921 gatggcatgc ccggcaatcg ccagctctgc ctgcgtgcgc ggcaacggct cgcccgacgc
     1981 cacgcgcaac tgccattcca ccagatcgag gccggtgatc atctcggtca ccggatgttc
     2041 gacctggagc cgcgtgttca tctccatgaa gtagaaatcg ctgcccgtgg cgatgaactc
     2101 cacggtcccg gcgccgcgat agtcgacggc gcgc
//
LOCUS       DATDEU010000094         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_746797, whole genome shotgun sequence.
ACCESSION   DATDEU010000094 DATDEU010000000
VERSION     DATDEU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746797"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2288)
                     /locus_tag="VIV63_02165"
     CDS             complement(<1..>2288)
                     /locus_tag="VIV63_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HEY6123428.1"
                     /translation="LEVARASGAEAIHPGYGFLSENADFAESCERAGLVFIGPTPAQM
                     RDFGLKHTARALAKECGLPLLPGTELLSDLPAALDAAETIGYPVMLKSTAGGGGIGMR
                     RCDTADELRESFAAVQRLAAGNFRNSGVFLEKFVARARHIEVQIFGDGHGAVIALGER
                     DCSVQRRNQKVIEESPAPGLDETTREALHAAAVRLGEKVGYRSAGTVEFVYDAAAAQF
                     YFLEVNTRLQVEHGVTEEVGGVDLVEWMIRTAEGSPPDLHEHWHVALGHAIQARVYAE
                     DPARGFRPSSGLLTNVQLPSDVRVEAWIDAGTEVTGYYDPMLAKIIARGATRELAITN
                     LANALRSSRIDGIETNLAYLVSILEHPVFAAGEQTTAMLQSHNPPAASIEVMAAGTLT
                     TVQDWPGRLGLWDVGVPPSGPMDGNALRLANRMVGNDEGAAALEMTVTGATLRFDSDA
                     VIALAGAAMEASIDGEPAPFWQPLAVRRGSVLALGRIVGAGQRAYLAVRGSFDIPEYL
                     GSRATFTLGQFGGHGGRALRAGDVLRLNRSGAELDGCRAIDAGEQPDYSHEWQLGVTD
                     GPHGAPDFFTEDDIRTFFDAEWEVHYNSSRTGVRLIGPKPTWARRDGGEAGLHPSNIH
                     DNAYAIGSVDFTGDMPVILGPDGPSLGGFVCPVTIVNEELWKIGQLRPGDKLRFRRVA
                     QWAARADNPSVVLELPASANHAAVCVRRAGESNLLVEFGAPVLDLVLRFQVQALYEQL
                     KARSLPGVRELTPGIRSLQVHF"
BASE COUNT          362 a          768 c          779 g          379 t
ORIGIN      
        1 cgaagtgcac ctgcagggag cggatgccgg gcgtgagttc gcggactccc ggtagggacc
       61 tggccttcag ctgctcgtac agtgcctgta cctggaagcg cagcaccaga tccagcaccg
      121 gcgcgccgaa ttccaccagc aggttgctct cgcccgcccg gcgaacacac acggcggcgt
      181 ggtttgcgga tgccggcaac tcgagcacga cggaggggtt gtcggcgcgc gcagcccatt
      241 gggcgacgcg gcggaatcga agcttgtcgc caggccggag ctggccgatt ttccacagct
      301 cctcgttgac gatggtgacc ggacacacga atccaccgag gctcgggccg tcgggtccca
      361 ggatcacggg catgtcgccg gtgaagtcga cactgccgat cgcgtaggcg ttgtcgtgaa
      421 tgttggacgg atgcagcccg gcttcgccgc catcgcgacg cgcccaggtg ggcttgggcc
      481 cgatgagccg cacgccggtg cggctcgagt tgtagtgcac ttcccactcg gcatcgaaga
      541 aggtgcggat gtcgtcttcg gtgaagaagt ccggcgcgcc atgcgggccg tcggtgacgc
      601 ccagttgcca ttcgtggctg tagtcaggct gctcgcccgc gtcgatggca cggcatccgt
      661 cgagttcggc tccactgcgg ttcaaccgca gcacgtcacc ggcgcgcaac gcacgcccgc
      721 catgacctcc gaactgtccg agcgtgaacg tggcgcggct gccgagatac tcgggaatgt
      781 cgaagctgcc gcgcacggcg aggtacgcgc gctgaccggc gcccacgatg cgcccgagcg
      841 cgagcacgct gcctcgccgc acggcgagcg gctgccagaa cggcgcgggc tcgccgtcga
      901 tcgacgcttc catggccgcg cccgcgagcg cgatcaccgc gtcgctgtcg aagcgcagcg
      961 tcgcgccggt gaccgtcatc tcgagcgccg ccgcgccttc gtcgttgccc accatgcggt
     1021 tggccaggcg cagcgcattt ccgtccatgg gtccggaagg cggtacgccg acatcccaca
     1081 accccagccg gcccggccag tcctgcaccg tggtcagcgt gcctgccgcc atgacttcga
     1141 tgctcgcggc cggtgggtta tgactctgca acatcgcggt cgtctgttcg ccggcggcga
     1201 acaccgggtg ttcgaggatc gacaccaggt aggcgaggtt ggtctcgatg ccgtcgatgc
     1261 gtgaactacg caaggcgttc gccaggttcg tgatggccag ctcgcgcgtc gcgccgcgcg
     1321 cgatgatctt ggcgagcatg ggatcgtagt agccggtcac ttccgtgccc gcgtcgatcc
     1381 atgcctccac acgcacgtcc gaaggtagct ggacgttggt gagcagcccg ctcgacggac
     1441 ggaagccgcg cgccgggtcc tcggcataga cgcgcgcctg gatcgcgtga ccgagcgcga
     1501 cgtgccagtg ttcgtgcaga tccggcggcg aaccttccgc cgtgcggatc atccactcca
     1561 cgagatcgac accaccgact tcttcggtga caccgtgttc cacctggagg cgcgtgttga
     1621 cctccaggaa atagaactga gcagccgcgg cgtcgtagac gaactccacc gtgccggcgg
     1681 agcgatagcc gaccttttcg ccgagacgca cggccgctgc gtgcagggcc tcgcgcgtgg
     1741 tttcgtcgag tcccggtgcg ggcgattcct cgatgacttt ctggttgcgg cgctggaccg
     1801 agcaatcccg ctcgccgagt gcgatcacgg cgccgtggcc gtcaccaaag atctgcactt
     1861 cgatgtgccg cgcgcgcgcc acgaattttt cgaggaacac accggagttg cggaaattcc
     1921 cggccgcgag ccgctggacg gccgcgaagg attcgcgaag ctcgtcggcg gtgtcgcaac
     1981 gtcgcatgcc gatgccgccg ccgccggcag tgctcttgag catgaccggg tagccgatgg
     2041 tttccgcggc gtcgagcgcc gccggcagat cggacagcag ctcggtgcca ggcagcaagg
     2101 gcaggccgca ttccttcgcg agcgcacgag ccgtgtgttt gagcccgaag tcgcgcatct
     2161 gcgcaggagt cgggccgatg aacacgaggc ccgcgcgttc gcagctctcg gcgaagtccg
     2221 cgttttcgga caggaatcca taaccggggt gaatggcttc cgcccccgat gcgcgagcga
     2281 cttctagg
//
LOCUS       DATDEU010000095        19960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1768122, whole genome shotgun sequence.
ACCESSION   DATDEU010000095 DATDEU010000000
VERSION     DATDEU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19960
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768122"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VIV63_02170"
     CDS             <1..461
                     /locus_tag="VIV63_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY6123429.1"
                     /translation="KHADPKRVCVVGGSFGGYLALATAVRDSALVKCVVSVAGVSDLL
                     ELKSDSNFFSNHQVVKEMIGKDPVKLKADSPRFNAASIAVPVLLIHGNDDYTVEVDQS
                     RFMAEALQAANKPHQFVVMPDTDHYFRKKQQMHDLFSSITGFLRAPLAAQ"
     gene            complement(466..1047)
                     /locus_tag="VIV63_02175"
     CDS             complement(466..1047)
                     /locus_tag="VIV63_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEY6123430.1"
                     /translation="MTLKYWLSVCAATTLLAACGQRDKPAETAEVPGTPSAPAPAESP
                     PPSAASAQLTPTQGNSASGTLALSVEGDSLRLTGSLQGLSPNGEFGFHIHENGDCSAP
                     DASSAGAHFNPANAQHGNPHGESHHAGDMLNAKSDAQGMATVDAVASGVSLESGVANS
                     VRGKAIVLHEKADDYASQPAGNSGARIACGVIN"
     gene            1315..3711
                     /locus_tag="VIV63_02180"
     CDS             1315..3711
                     /locus_tag="VIV63_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123431.1"
                     /translation="MKSWIPRGAAALLIAVPFASSAQEASPTDDEITEEIIVTAQKRA
                     TSLQDVPFSVAAVTAEDLEQSGATNIVELARSVPGLYIADLGPGQSQVAIRGISAGQV
                     VRDQAGVKESVGIYLDESPISVALFTPDHDLYDLDRIEVLRGPQGTLFGAGSSSGTVR
                     YITAQPNVGELGGSVDLTFNSLTDGEYGGSIRGSLNVPIGENSAMRAVGYISELPGFI
                     DAHYPGRTTNEDVNGGSRTGGRLAFRFEPSENFTITPRIVYQKLETDGYPRIDAYNIL
                     GNVYTTTEPRVDPSDRGQVTQFQEGLTDEFTMGDLKLDFDFGNVGMTSVTSYIDRTVE
                     VIRDASQLTGSVTKDLGGTDAQARLNSPLIDSTDLSVFSQEIRFASTGEGPFQWLAGA
                     FYQEAERDYGQNLPTPGYDALSQSLIGADSSDFGSPPDTPFYSDLSYDFSQFALFGEA
                     TYRFSPQWALTGGLRYYDFSEDRTLTFAGLFADQGYTDEPGSVSSDGFSPRVILAFSP
                     NENVQLTAQVARGFRLGGINDPLNVTLCSPADLVTYDGRENWEDEEVTNYEIGAKTRS
                     SDGRVTFNAAVFMSDIEGLQVIADAGSCSSRIILNAEAESVGAEMELYVRPDEHWDLG
                     LSATYVQAEITESQLASGTPIAGIRDGNRLPTSPELQAAASVAYNWSMSDSLTSYVRL
                     TLQHVGSSYTQLADQEPNFGLISNSATRPPGSARLINLGGIPSNTTIAFDAELPAYDI
                     ANLRWGIGTDSWEAALFVNNLWDELAFLSIDRERGRSARVGYLTNPPRTYGVNFHMNF
                     "
     gene            complement(3759..5240)
                     /locus_tag="VIV63_02185"
     CDS             complement(3759..5240)
                     /locus_tag="VIV63_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6123432.1"
                     /translation="MKRREFCASSLAVIASTGFSMHARAAVPDELPILSRTGKQLLLT
                     RAEVEELRSGLHGALLLNGDSGYEQARRIWNGAFDRRPAAIVRCADAADVVSAVQFAR
                     SHDVLVAVRGGGHSLPGHSVCEGGLMIDLAPMQGVEVDRKARTVRVDPGVWLGTMDRE
                     LHKHGFIVPAGTVSHTGVAGLSLGGGIGRLSRKFGMTIDNMLGANLVTPDGKLRRVDA
                     QQNPDLFWAIRGGGGNFGVVTSFDFRVHELGANVVGGDLVFPINEARAVLDHIADNFA
                     RAPDELWVDPVLECDANGARQLMLNVCHCGDSRAARKDIDALRKAGKIVRDTVGARSF
                     VKLQSEHDDDSPRGRSYYMSGAGVVDLEPALLDHSVRMIQEPGAELGKISITQSGGAM
                     ARVPMEATAYASRNAQFTVVVRAAWENPEHAAVRTAWQKETSKGVQPFARSIYANLNL
                     GEGDPRLLGAYGPNTKRLVDLKTQYDPTNLFHMNPNIPPRAAS"
     gene            complement(5353..5694)
                     /locus_tag="VIV63_02190"
     CDS             complement(5353..5694)
                     /locus_tag="VIV63_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123433.1"
                     /translation="MLLVIAAISAAVSVLLAGRMPPAMASFRDTFASLGVVPPPSAVL
                     VFRYSQVWWVLAVGAVAVFVWIAMRASAEATELKRMKLALCLWILLTALVYGFTAYAI
                     YTPLSGLGAAI"
     gene            complement(5715..6878)
                     /locus_tag="VIV63_02195"
     CDS             complement(5715..6878)
                     /locus_tag="VIV63_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6123434.1"
                     /translation="MSKSAAWDSIVVGAGVFGAWTAWHLRKAGQRVLLLDAFGAAHAR
                     ASSGGESRLTRGSYGRDEVYTRFAFDTLAQWKWLSDLSGLPVFHPLGVLFFFQKREAY
                     VDESLEVHRRLKLPTVELDRAAMEQRYPQMNWKDIEVGLFEPGFGVLMARRAVQTLVK
                     QFVKAGGEYRVAAVKPPDASKPVTSLELSDGSAVSAANYVFACGPWLPKVFAHLLGPR
                     IFPTRQEVFFFGTPAGDARFGAGSLPGWADFNNGDIFYGMPDLEGRGFKIAHDKHGPP
                     IDPDRGDRVASPGALADVRAYMAKRFPALASAPLNEERVCQYENSSNGDLLIDRHPSA
                     GNLVLVGAGSGHGFKHGPEVGRYAAELLTGKLKIVEPRFSLATKAEQKNRAVH"
     gene            complement(6955..10374)
                     /locus_tag="VIV63_02200"
     CDS             complement(6955..10374)
                     /locus_tag="VIV63_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6123435.1"
                     /translation="MKPSVPISRLIVMLLAGVSAVSAQTLELHQADRQPTPVIDPASD
                     DATLAIQRFSLPAGMQAKLWAAEPMLANPVAFDFDEKGRLFVSETYRYRSSVLDIRDY
                     MGMLELDLAARTIEDRVALHHKVFGAQAKDFAIEGEVVRLVEDTNGDGVADKSRAFAD
                     GFNSELDGIASGVLARHGKVWFTNIPSLWLLEEGADGKVKKDELLRGFGVRYNFTGHD
                     FHGLAMGHDGKIYYSIGDRGTHVKGKEGQVVAYPDEGAVFRSNPDGTEFELVAHGLRN
                     PQELVFDEHGNLFTGDNDSDQGDMERLVYLVEGGDSGWRVGYQHAPLGKGGPWMREGL
                     WKPAFAGRPAYLLPPVCNIEDGPSGLTYYPGTGLDPKYAGHFFITHFKGSIARSGIQT
                     YTLKQNGATFTPTSSQQFMGGVLPTDVTFAPDGKLYILDWVDGWPKSNKGRVYGIAPA
                     LQDPAQVKISADLAALLAAGFKNKDAATLAGLLAHPDRRARLEAQLELASRGPASVKV
                     FTTVANNQKAAPLARLHAVWGLTQLGRQKANVGPTLVKLLADSDGEVRAQSAKGLGDF
                     KFAKAQAALVKSLTDAEPRVQFFAAQSLGKLKDAKSTAPLLALLRTNDNKDAYLRHAA
                     SHALAGIGKNATLDAGVSDPSAAVRLGVVLAYRKLRDASISAFLNDSDAYVAREAATA
                     INDAPIEAAYSALAAKLDNAPVADEPFVERAINAHYRVGATANAQALAQYATRNGASS
                     GMRAEALAQLGLWGKGPQRDRIVGIYRPLAARGAKDAADALTPVLPKVLGQGPEDVQL
                     AAIEAIGNLNLAGAAPTLVATVANESGAEAVRVSALKALNALGGNDWMAAVSAAEKSS
                     NATLRLAALQIVASRSPERALPIVRRLTTSTTEAEQQAAFTALGQLKTAETPKLLVAA
                     LDQLAAGKVQPGAQLELITAAEASDAPAVKARWEKQQAAWAASGKALAPYTFALAGGN
                     PWRGAREFYGNPVLPCARCHKVNGEGGEAGPELTLIGAQKSKEYLLESIIKPSAHIAA
                     GFDVVTLQLKNGVTETGSITTETPTEIVLKRADNSSATIDPKQVKERVTAPSSMPEIY
                     AQVLTRAQLRDLVAFMGVLTRSDRGDGEVPFGQSNRAMSSTTQESKAGGHP"
     gene            10491..11267
                     /locus_tag="VIV63_02205"
     CDS             10491..11267
                     /locus_tag="VIV63_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123436.1"
                     /translation="MNYSRRVNKWVALAIASIGVLVAWFSQREAGKSPPGTAPDNVDI
                     PAPKPAGADFDFYLMALTAHPAFCADGNSRRQECRGGGHRSLVIHGLWPERMEPRTYP
                     RDCPAPPLDLEPALVQQLGELMPGMADGLHVHEWRTHGGCSGLGDDEYYGRALELART
                     VDAALGAKLTTLAGGETTPGELRGVADLFEPGLGRTLTFHCRTLRDGGNRRPYLFEVR
                     QCVDNDGPGGAPRTALVCATMKRRDQGCGASFMIAGEALR"
     gene            11264..11569
                     /locus_tag="VIV63_02210"
     CDS             11264..11569
                     /locus_tag="VIV63_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123437.1"
                     /translation="MNFSAQLVELAIAYGVTAAAMLAGLLLMRADSRAYFRWPVYSAM
                     AMVLGVVSWNLARKHGLPPEWGATHANAMYYGALAVYATLGFGLGLLVGRITQSKTE"
     gene            11600..11782
                     /locus_tag="VIV63_02215"
     CDS             11600..11782
                     /locus_tag="VIV63_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123438.1"
                     /translation="MAKSKKKTPSKKEVKKLRKEINAVTKAFLARYDYDFLMVGPPGP
                     RPPPKLEISILNLSSY"
     gene            complement(11784..14024)
                     /locus_tag="VIV63_02220"
     CDS             complement(11784..14024)
                     /locus_tag="VIV63_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY6123439.1"
                     /translation="MDRREIRFDGWKVNFDSGEISRDGNTHRLQDQPLQILDELVKHP
                     GEVVSREQLIARLWPTGVVEFDTGLNSAMRKLRIALGDDADTPRYIETIPRKGYRFVG
                     HLEDPPLPMAGIPTLPAQYVPTSFETGAVIGRRASDRRAPLKRLALGFGSVVAALILG
                     LVAWRMPGNLFQVDVVTESFPTIVVLPLVDMSVDQSEQALCDGLTEELSNWLAHIPTL
                     RVVARTSAFAFKGQNIDVRKIAEALGASHVLEGSLRRSGDQLRITVQLIAADTGLHIW
                     SDSFDHPMGNIFRIEDSVSRAVAEKLHLELTAETAEVWAQRQPQRMEAHELYLLGRAR
                     QSRRTADDNLKSIEYFRRAVNADPKYIPALVGLSESLLNGLSLNRAPIEDVKAEVEPL
                     INRAMHINPNLAEVLAVKGWLLNEEFRSDEALPLLLRATKLNPNDASSHRFLGDLFDR
                     RAEPGKALTHFSASAALDPMDFISHVFRCIELIDLGDYAEATAACTRARELDPTHMWG
                     PLATSWIARAQGKTDEAIQWIDTARKLQPVDAYLADQKIEMLLAEAKFADARLVMGSL
                     PADGSFFSLARESNIVYAEGGGDAVKAWMVGRDLTSRAGTGAELVELARMQLVAGDPA
                     AARTTLVHAERVLPMSSADMFDGSQIRHEYSVALLLAGIELKGGGDRNRALKLLSELD
                     DMLDRYEENGGKHFGMYRLRAESLAMQGKMPEAQAALNTAWQKGWRNVWRARREPYFT
                     GLDLPK"
     gene            14140..14844
                     /locus_tag="VIV63_02225"
     CDS             14140..14844
                     /locus_tag="VIV63_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123440.1"
                     /translation="MNVTAHRSIGPAPLPGPEPQPDAPGQPGPRPPPKHLSARALTAL
                     VSQLTTGYPNPDSPEDTPGPLDPYIRKALERSVIGAGTSGYLWRVIAEKHPEIWDVIG
                     GDPHTQVALNPQPLPPKSAFLAAVILEFTERMTIVAEIADLIPRPGGERGIIIVGGHV
                     ARFVDDICGNGLHIKWPLPWPSPPWFKDTLSGADLIVMGTQFQQSAVIAMDRDLGRTF
                     ADAAHALLEAGAARLV"
     gene            complement(14943..15764)
                     /locus_tag="VIV63_02230"
     CDS             complement(14943..15764)
                     /locus_tag="VIV63_02230"
                     /inference="COORDINATES: protein motif:HMM:NF039735.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6348 family protein"
                     /protein_id="HEY6123441.1"
                     /translation="MFSWFRKSPRPPRPATGNPGQGHIVRVAFSNAQKTWQETDDLTT
                     SLSAALKAQGHEATAKGDWVELGDFSLLPQVVGVEPLENSGVKTVSTIQVSHPTLLQG
                     SLFEYQHSSGTDVRDSFAKGFKNWAELDLPVFLDAQRPKAVDCMVMEMKPGAESTSVL
                     PVDRRIVFGPPLQMAQNQDVVAGDHDFCPCCLFTNSIAAFDDLLRDQNFYGVRLFVSR
                     DDQGLIKADCRVNGVDRPEGAAALVRYAQTWPDRGFEYRKQYVCIQARPEPQVVS"
     gene            15834..17804
                     /locus_tag="VIV63_02235"
     CDS             15834..17804
                     /locus_tag="VIV63_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HEY6123442.1"
                     /translation="MSYFRLSFVASWALFASAAPAIAAPSVSVASPDGRIVVAVSLAD
                     ARATYTVSVGGKEILRQSRLGVVRDDADFTTGIDATANYEKRAAKLEKVEDRYELLTS
                     KRRQNVYRANRRVLEFKTPAGARMDIEFQVSNDGYAFRYVFPETDATVRKISREASSF
                     NFLAGTRGWLQPIAPARSGWNETNPSYEEYYERDIPAGQPSLQGGAWVFPALFRSGDT
                     WLLVSEAGLRRNYCGSRLLAARRSSEYFIDFPGPLEASNGGAVTPESTLPWITPWRFV
                     VIGDLKTIAESTLGTDLADPPAKGFKTTASGPGKSSWSWPLLGDDQTIIKVQKRFVDY
                     AAEMGWQYTLVDSAWDRQIGYDGLKELVEYGRPKNVKILIWYNSAGEWNTTPLTPRDR
                     MHDPATRRAEMQKIKDLGIAGVKVDFFAGDAQSTIAYYHDILEDAARIGLAVNFHGAT
                     LPRGWQRTYPNLMTMESVRGLEFNTFEQRNAEESATHDAMLPFARNVFDPMDFTPVVL
                     DRLNNVVERRTSAAFELATAVLFTSGIQHYAEIPQGMAKAPVYVRDFLKKVPAVWDDV
                     RFIDGFPGQYAVFARRAGNTWYVAGINADRQQPRKVKIDLKALGVAKQGTLITDGIDF
                     LGFKSETFPLEKGATSDEITMRPRGGFVLTFN"
     gene            17874..18218
                     /locus_tag="VIV63_02240"
     CDS             17874..18218
                     /locus_tag="VIV63_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6123443.1"
                     /translation="MVDEDLVFKALADSSRRFLLDLLHKRDGQTLGELEWELEMTRFG
                     VMKHLKVLEEAGLIVTRREGREKLHFLNAVPIRLIHDRWIDKYKERELSALADLKSTL
                     EKTKCPPLRKRA"
     gene            18227..18709
                     /locus_tag="VIV63_02245"
     CDS             18227..18709
                     /locus_tag="VIV63_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY6123444.1"
                     /translation="MNSTTHIHQIYIKADPARIWDAITSPEWNGKYGYGGVSHYDLKP
                     GGKFKCIANEQMRSFGLPEVIVDGEVIEVKPPYKLVQTYRFLFNEEHKKEGFTRISFE
                     IEQTSGGFCRLTITHDTAGAPMMAGATQSNFSQEGGGGWDWILSDLKSLLETGKTMSD
                     "
     gene            18812..19036
                     /locus_tag="VIV63_02250"
     CDS             18812..19036
                     /locus_tag="VIV63_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY6123445.1"
                     /translation="MKTLRLKVKPGSREEGLEELDDGSWLARVKAPPVDGKANTAVIA
                     LVARHFGLRKAQVNIKSGASGRMKLVVLED"
     gene            19042..19749
                     /locus_tag="VIV63_02255"
     CDS             19042..19749
                     /locus_tag="VIV63_02255"
                     /inference="COORDINATES: protein motif:HMM:NF028481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6123446.1"
                     /translation="MITLYTFGPAFGLPDPSPFVMKGEMLLKLAKLDYQPDTKGFLRA
                     PKGKLPFIDDNGTIVADSTLIRLHLEQKHGVDFDRGLSMRDRGIAWATEKMLEDHLYW
                     VLVYWRWMKDDNFERGPANFFKRAPAFIRPLVKWKIRGNVRRSLHAHGISRHNETEMT
                     AMSDRAFEALSRVLGENKYLMGNEPCGADATAFAFVAGALTPVFESPAHGKARTLPNL
                     IAYRDRMMSEFYPGFKY"
     gene            19775..>19960
                     /locus_tag="VIV63_02260"
     CDS             19775..>19960
                     /locus_tag="VIV63_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123447.1"
                     /translation="MKLVRLIATALFALQISLASFADGSGSATRAAFLQVIDRPRVAL
                     KAELAEMAPVEGFRKYHL"
BASE COUNT         3549 a         6504 c         6382 g         3525 t
ORIGIN      
        1 agaaacatgc cgacccgaag cgtgtgtgcg tggtgggcgg cagcttcggc ggatacctcg
       61 cgctggctac cgccgtgcgc gacagcgcgc tcgtgaaatg cgtggtgagc gtggccggcg
      121 tgagcgacct gctcgagctc aagagcgatt cgaacttctt ctccaaccac caggtcgtga
      181 aggaaatgat cggcaaggac ccggtgaagc tcaaggcgga ctcgccgcgg ttcaatgcgg
      241 cttcgatcgc cgtgccggtg ctgctgattc atggcaacga cgactacacc gtcgaggtcg
      301 accagtcgcg gttcatggcc gaagcgctgc aggcggcaaa caagcctcac cagttcgtgg
      361 tcatgccgga cacggatcac tacttccgca agaaacagca gatgcacgac ctcttcagct
      421 cgatcaccgg gtttttgcgc gcgccgctcg ccgcgcagta ggcggtcagt tgattacacc
      481 gcaggcaatg cgcgcgccgg agttgccggc cggctgactc gcgtagtcgt cggccttctc
      541 gtgcagcacg atcgccttgc cccgcacgct gttggccacg ccactctcga gactcacgcc
      601 gctcgccacc gcgtcgaccg tggccatgcc ctgcgcatcc gacttcgcgt tgagcatgtc
      661 gccggcgtga tgactttctc cgtgcggatt gccgtgctgg gcgttggccg gattgaagtg
      721 cgcacccgcg ctgctggcgt cgggcgcgct gcagtccccg ttctcgtgaa tgtggaagcc
      781 gaactcgccg ttcggtgaga gcccctgcag cgacccggtg agtcgcaacg agtcaccttc
      841 caccgacagc gcgagagtgc ccgacgcgct gttgccttgg gtcggtgtga gctgcgccga
      901 ggcagccgag ggcggaggcg actccgccgg cgctggcgcg gagggtgtgc ccggcacctc
      961 cgccgtctcg gcgggtttgt cgcgttgccc gcatgcggcg agcagggtcg tggcggcaca
     1021 gacggatagc cagtacttca aagtcatgct gactccagga tcatgggttg aggtggaggc
     1081 accctatgaa ggtcggcgca cagggtcagt cggcgggtgg cccgattcgg cgagacgatg
     1141 tctgactccc acgtgccgtt gtgcgcaagc gtcgccggat ccaccccata tgcggctacc
     1201 tggcagacga cagcgacttt tccgctacac tccgcctcac aagtgcgcag tccgggggga
     1261 tggtcgctca cgaccccgct gccgtacaca aataacgaca gaaggagtgg tcgcatgaaa
     1321 tcgtggatcc cgcgcggcgc agccgcgttg ttgattgccg tgccctttgc atcgtcggcg
     1381 caggaagcct cgccgaccga cgacgaaatc accgaagaaa tcatcgtcac ggcgcagaaa
     1441 cgcgccacct cactgcagga cgtgccgttc tcggtcgccg ccgtcaccgc cgaagacctc
     1501 gagcagtccg gcgccaccaa catcgtcgag ctcgcccgca gcgtccccgg cctctatatc
     1561 gccgatctcg gcccgggtca gagccaggtc gccatccgcg gcatcagcgc cggacaggtg
     1621 gtgcgagacc aggccggtgt gaaggaatcg gtgggcatct acctcgatga atcgcccatt
     1681 tccgtagcgt tgttcacgcc ggaccacgac ctgtacgacc tcgaccgcat cgaagtactg
     1741 cgcggcccgc aaggcacgct gtttggcgcg ggttcgtctt cgggcaccgt gcgttacatc
     1801 accgcgcaac ccaatgtcgg cgagctcggc ggttcggtgg acctgacctt caactcactc
     1861 accgacggtg agtacggcgg ctcgatccgc ggcagcctca acgtgccgat cggcgagaac
     1921 tccgcgatgc gggccgtggg ttacatcagc gaactaccgg gattcatcga cgctcactat
     1981 cccgggcgga cgaccaacga agacgtgaac ggcggctcgc gcaccggcgg acgtctggct
     2041 ttccgcttcg aaccgtcgga aaacttcacg atcacgccac gcatcgtcta ccagaagctc
     2101 gagacggatg gctatccgcg catcgacgcc tacaacatcc tcggcaacgt ctacaccacg
     2161 accgagcccc gggtggatcc gagtgatcgc ggacaggtca cgcaatttca ggaaggcctc
     2221 accgacgaat tcaccatggg cgacctcaag ctcgatttcg atttcggcaa tgtgggcatg
     2281 acgtcggtca cgtcgtacat cgaccgcacc gtcgaagtca ttcgcgatgc cagccagctc
     2341 accggcagcg tcaccaagga cctgggtgga accgacgcgc aggcgcggct caactcgccg
     2401 ctgatcgact ccacggatct gtcggtgttc agccaggaaa tccgcttcgc ctcgacgggc
     2461 gaaggaccgt tccagtggct ggcgggcgcg ttctaccagg aggcggaacg cgattacggc
     2521 cagaacctgc cgacgccggg atacgacgcg ctttcgcaat cgctcatcgg cgccgacagt
     2581 tcggatttcg gctctccgcc cgacacgccg ttctattccg acttgagcta cgacttcagc
     2641 cagttcgcgt tgttcggcga agctacctac cgcttcagcc cgcagtgggc gttgaccggt
     2701 ggcctgcgct actacgactt cagcgaagat cgcacgctga cgttcgccgg cctgttcgcc
     2761 gatcagggct acaccgacga gccgggttcg gtgagctcgg atggcttttc accacgcgtc
     2821 atcctcgcgt tcagccccaa cgagaacgtg cagctgaccg cgcaggtcgc gcgcggcttc
     2881 cggctcggcg gcatcaacga tccgttgaac gtcacgttgt gctcgcccgc ggacctcgtg
     2941 acctacgacg gtcgtgagaa ctgggaagac gaagaggtga ccaactacga gatcggcgcc
     3001 aagacccgct ccagcgatgg ccgtgtcacg ttcaatgccg cggtgttcat gagcgacatc
     3061 gaaggactgc aggtgatcgc ggacgcaggc tcatgctcct cgcgcatcat cctcaacgcc
     3121 gaagccgagt cggtcggtgc ggaaatggag ctgtacgtgc gtcccgacga acattgggac
     3181 ctcggtctgt cggctaccta cgtgcaggcc gagatcaccg agtcgcaact cgcgagcggc
     3241 acgccgatcg ccggcatccg cgacggcaac cgcctgccga cttcgcccga gctgcaggcc
     3301 gcggccagcg tggcttacaa ctggtcgatg agtgactcgc tgacgagtta cgtgcggctc
     3361 acgctgcagc acgtcgggtc gtcgtacacg cagctcgcgg accaggagcc caacttcggc
     3421 ctgatctcca acagcgccac acgtccgccg ggctccgcgc gtctgatcaa cctgggtggc
     3481 attccctcca acaccaccat cgcgttcgac gccgagttgc ccgcgtacga catcgccaac
     3541 ctgcgatggg gcattggtac ggactcgtgg gaagccgcgc tgttcgtgaa caacctgtgg
     3601 gatgaactcg cgttcctgtc catcgatcgt gaacgcggcc gcagcgcgcg cgtggggtac
     3661 ctcaccaacc cgccgcgtac ctacggcgtg aatttccaca tgaactttta atggggacat
     3721 ggatgggtgg ggacagactt aagtctgtcc ccacactttt atgacgccgc gcgcggcgga
     3781 atgttcgggt tcatgtggaa cagattggtc gggtcgtact gcgtcttgag atcgaccaat
     3841 ctcttcgtat tcggcccata agctcccagc agacgcgggt cgccttcgcc gagattgaga
     3901 ttcgcataga tgctgcgcgc gaatggctgg acccctttcg aagtctcctt ctgccacgcg
     3961 gtgcgaacgg ccgcgtgttc ggggttctcc catgccgcgc gcaccaccac ggtgaactgc
     4021 gcattccgac tggcataggc ggttgcctcc atcggtaccc gcgccatcgc gccgccgctc
     4081 tgcgtgatgc tgatttttcc gagctccgcg cccggttcct ggatcatgcg caccgagtgg
     4141 tcgagaagcg cgggctcgag atccaccacg ccggcgccac tcatgtagta gctgcggccg
     4201 cgcggcgaat cgtcgtcgtg ctcggattgc agtttgacga acgaccgcgc gcccactgtg
     4261 tcacgaacga tcttgcccgc cttgcgcagc gcatcgatgt ccttccgcgc ggcccgcgaa
     4321 tcgccgcagt ggcaaacgtt gagcatgagc tggcgcgcgc cgttcgcgtc gcactcgagc
     4381 accgggtcga cccacagttc gtccggagcg cgagcgaaat tgtcggcgat gtgatcgaga
     4441 accgcccggg cctcgttgat gggaaacacg aggtcgccgc ccaccacgtt cgcgcccagt
     4501 tcatgcacgc gaaagtcgaa ggaagtcacg acgccgaaat ttccgccgcc gccgcgaatg
     4561 gcccagaaga gatcgggatt ctgctgcgca tcgacgcgac gcagctttcc atcgggcgtg
     4621 acgagattgg cgcccagcat gttgtcgatg gtcatgccga actttcgcga caaccggcct
     4681 atgccacctc cgagcgacag accggcgaca ccggtgtgcg acaccgttcc cgccggcacg
     4741 atgaacccgt gtttgtgcag ctcgcgatcc atcgtcccta accacacgcc gggatccacg
     4801 cgcactgtgc gcgccttgcg atcgacttcg acgccctgca tgggcgcgag atcgatcatg
     4861 aggccgcctt cgcagaccga gtggcccgga agactatgtc cgccaccgcg aacggccacc
     4921 agcacatcgt gcgaacgcgc gaactgcacc gcgctcacga catcggcggc gtcggcgcag
     4981 cgcacgatgg ccgcgggccg gcgatcgaac gcgccattcc agatgcggcg agcctgctcg
     5041 taacccgagt cgccgttgag cagcagggcg ccgtggaggc ctgatcggag ttcttcgacc
     5101 tccgcccgcg tcagcagcaa ctgcttgccc gttcgcgaca ggatgggcag ttcatcgggc
     5161 accgcggcgc gagcgtgcat cgagaagccc gtcgaggcga tgaccgccag cgacgaagcg
     5221 cagaattcgc gtcttttcat gtttcttgct cccatcggga ccagcactat cgcgctgcac
     5281 gacacgcagc gccacgtgcc ggcgcggcga agatcaacgg aatgtgaaaa aagccgcgcg
     5341 agcgggcggc tctcagatcg cggcgccgag gccggacagg ggcgtgtaga tggcgtacgc
     5401 ggtgaagccg tagaccagcg ccgtgagcag gatccacagg catagcgcga gcttcatgcg
     5461 cttcaactcc gtcgcttcgg cactggcgcg catggcgatc cagacgaaca ccgcgacggc
     5521 gccaaccgcc agcacccacc agacctggga atagcgaaaa accagcaccg cggacggagg
     5581 cgggaccacg cccagggacg caaaggtgtc gcgaaagctg gccatcgccg gcggcatgcg
     5641 accggcgagc agcaccgata ccgcggcgga aatggccgcg atgaccaaca gcaagcggga
     5701 gcgcggcagc gcgctcaatg cacggcccgg ttcttctgct ctgcctttgt cgcgagactg
     5761 aatcgcggtt cgacgatctt caacttgccc gtcaagagtt ccgccgcgta tcggccgact
     5821 tccggcccat gcttgaaccc atgacccgac cccgcgccga ccagcacgag attcccggcc
     5881 gacggatggc gatcgatcag caaatcgccg ttgctgctgt tttcgtactg gcacacacgc
     5941 tcttcgttga gcggcgccga cgcgagcgcc ggaaaacgct tcgccatgta agcgcgcaca
     6001 tcggccagtg cgcccggtga cgccacgcga tcgccgcgat cgggatcgat gggcgggccg
     6061 tgtttgtcgt gcgcgatctt gaagccgcgg ccttcgaggt ccggcatgcc atagaagatg
     6121 tcgccgttgt tgaagtccgc ccaacctggc agggagccgg cgccgaagcg ggcatcgccc
     6181 gcgggtgtgc cgaagaagaa cacttcctgg cgcgtcggga aaatgcgcgg gccgagcagg
     6241 tgcgcgaaca ccttcggcag ccagggtcca cacgcgaata cgtagttagc cgcggacacg
     6301 gctgaaccat cggacagttc gagcgacgtc accggcttcg atgcgtccgg tggcttcacg
     6361 gccgccacgc gatattcgcc accggccttc acgaactgtt tgaccagggt ctgcaccgcg
     6421 cgtcgcgcca tgagcacacc gaacccgggc tcgaacagac ccacttcgat gtccttccag
     6481 ttcatctgcg gataacgctg ttccatcgcc gcgcgatcga gctccacggt cggcagtttc
     6541 agccgacggt gcacttcgag cgattcatcg acgtaggcct cgcgcttctg aaagaagaac
     6601 agcacgccca gcggatggaa caccggtagt cccgaaagat ccgacagcca tttccactgc
     6661 gccagcgtat cgaacgcgaa gcgcgtgtag acctcgtcgc ggccgtaact gccgcgcgtg
     6721 aggcgcgatt cgccgcccga cgacgcgcgc gcatgcgctg cgccgaatgc gtcgagcagc
     6781 aacacgcgct gtccagcttt gcgcaggtgc cacgcggtcc aggcgccgaa cacgccggcg
     6841 ccgacgacga tggaatccca agccgctgac ttgctcactt ttcgctcctc accgtgcacg
     6901 gcgcccgcga cactggccgc gagcgccgtc gcaatgacgc cgcgccgcga gaccttacgg
     6961 atgcccacca gctttcgatt cctgcgtcgt cgagctcatc gcccggttgc tctggccgaa
     7021 cggcacctcg ccatcgcccc ggtcggaacg cgtgagcacg cccatgaagg ccacgagatc
     7081 gcgcagctgc gcgcgtgtca gcacctgggc gtagatctcg ggcatcgacg aaggcgccgt
     7141 gacgcgctct ttaacctgct tcggatcgat ggtggccgat gagttgtcgg cacgcttgag
     7201 cacgatttcc gtgggcgtct cggtggtgat ggagcccgtc tcggtgacgc cgttcttgag
     7261 ctgcagcgtg accacgtcga agcccgcggc gatgtgcgcg ctcggcttga tgatgctttc
     7321 gagcaggtac tccttgctct tctgcgcgcc gatgagcgtg agctccgggc ccgcttcgcc
     7381 gccttcaccg ttcaccttgt ggcatcgcgc gcagggcagc accgggttgc cgtaaaactc
     7441 gcgcgcgccg cgccacggat ttccgccggc cagcgcgaac gtgtacggcg cgagcgcctt
     7501 gccgctcgcg gcccaggccg cctgttgttt ctcccagcgc gctttcacgg ccggagcatc
     7561 gctcgcctcg gctgccgtga tcagttcgag ctgcgcgccc ggctgcacct tgccggccgc
     7621 gagctgatcg agcgccgcca cgagtagttt gggtgtctcg gcggtcttca gctggcccag
     7681 cgccgtgaac gcggcctgtt gctcggcttc ggtggtgctg gtcgtgagac ggcgcacgat
     7741 gggcagtgcg cgttcgggtg agcggctcgc gacgatctgc agcgccgcga ggcgcagcgt
     7801 ggcgttgctg gatttctcgg cggcgcttac cgcggccatc cagtcattgc caccgagcgc
     7861 gttgagcgcc ttgagcgcgc tgacccgcac ggcttcggcg ccgctttcat tggccacggt
     7921 ggcgaccagc gtcggcgctg cgccggccag attcagattg ccgatcgcct cgatggctgc
     7981 gagctgcacg tcttccggtc cctggccgag aaccttgggc aacaccggcg tcagcgcatc
     8041 cgcggcgtcc ttcgcgccac gtgccgccag cggacgatag atgccgacga tgcgatctcg
     8101 ttgtggccct ttgccccaca agccgagctg tgcgagcgct tcagcgcgca tgccgctgct
     8161 cgcgccgttg cgcgtggcgt attgcgccag cgcctgtgca ttcgcggtgg caccgacccg
     8221 gtagtgcgca ttgatcgcgc gttccacgaa gggctcgtcc gcgacgggcg cgttgtcgag
     8281 tttcgcggcg agcgcggagt aggcggcttc gatcggcgca tcgttgatgg ccgtggccgc
     8341 ctcgcgagcc acgtacgcat ccgaatcgtt gaggaacgct gagatgctcg catcgcgcag
     8401 tttgcgatag gcgagcacga cacccaaccg cacggcagcg gaggggtcgc tcacgccggc
     8461 gtcgagcgtc gcgttcttgc cgatgcccgc cagcgcatgc gaggccgcgt gccgcaggta
     8521 ggcgtccttg ttgtcgttcg tgcgcagcag cgcgagcagc ggcgccgtgg acttcgcgtc
     8581 cttgagcttg ccgaggctct gcgcggcgaa gaactgcacg cgcggttctg cgtcggtgag
     8641 gctcttcacg agcgccgcct gtgccttggc gaacttgaag tcgcccaggc ccttcgccga
     8701 ttgcgcgcgc acttcaccat cggagtcggc gagcagcttc acgagagtcg ggccgacgtt
     8761 cgccttctgg cggccgagct gggtgagccc ccacacggca tgcagacgcg ccagcggcgc
     8821 tgccttctgg ttgttagcca ccgtggtgaa caccttcacg cttgccggcc ctcgcgaggc
     8881 gagttcgagc tgagcctcga ggcgcgcgcg gcgatcggga tgcgccagca ggcccgcgag
     8941 cgtggcggcg tccttgttct tgaagcccgc cgcgagcagc gcggcgaggt cggcgctgat
     9001 cttcacctgc gcgggatcct gcaacgccgg cgcgatgccg taaacccggc ccttgttcga
     9061 cttcggccag ccatccaccc agtcgaggat gtacagcttg ccgtcgggag cgaacgtcac
     9121 gtcggtgggc aacacgccgc ccatgaactg ctggctgctg gtgggcgtaa acgtcgcgcc
     9181 gttctgcttc agcgtgtagg tctgaatgcc gctgcgagcg atgctgccct tgaagtgcgt
     9241 gatgaagaaa tgcccggcgt acttcgggtc caggcccgtg cccggatagt aggtgaggcc
     9301 agagggtcca tcctcgatgt tgcacaccgg cggcagtaga taggcaggac gcccggcgaa
     9361 cgcgggcttc cacaggcctt cgcgcatcca cgggccaccc ttgccgagcg gcgcatgctg
     9421 gtaaccgacg cgccagccgc tgtccccgcc ttcgacgagg tacaccaggc gctccatgtc
     9481 gccctggtcg ctgtcgttgt cgccggtgaa caggttgccg tgttcgtcga agaccagctc
     9541 ctgcggattg cgcaggccgt gcgcgaccag ctcgaattcc gtgccatcgg gattggagcg
     9601 gaacaccgcg ccttcatccg gataagcgac gacctggcct tccttgccct tcacatgggt
     9661 gccgcgatcg ccgatcgagt aatagatctt gccgtcgtgg cccatggcca ggccgtgaaa
     9721 gtcgtggccc gtgaagttgt agcggacgcc gaagccgcgc agcagctcgt ccttcttcac
     9781 cttgccgtcg gcgccttcct ccagcaacca gaggctggga atattcgtga accagacttt
     9841 gccgtggcgc gcgagcacac ccgacgcgat gccatcgagc tcgctgttga agccgtcggc
     9901 gaacgcacga ctcttgtcgg ctacgccgtc gccgttcgtg tcttcgacga ggcggaccac
     9961 ttcaccttcg atcgcgaagt ccttcgcctg cgcgccgaac accttgtgat gcagggcgac
    10021 gcggtcttcg atcgtgcgtg ccgcgagatc gagttcgagc atccccatgt aatcgcggat
    10081 gtcgagcacg ctgctgcggt atcgataggt ttcggagacg aacaggcgtc ccttctcgtc
    10141 gaaatcgaac gccaccggat tcgccagcat gggctcggcg gcccacagct tcgcctgcat
    10201 gcccgcgggc aacgagaacc gctggatggc gagtgtcgcg tcgtcggacg cggggtcgat
    10261 gaccggcgtc ggctgccggt cggcctgatg caactcgagc gtttgcgcgg agaccgccga
    10321 cacaccggca agcaacatca cgatcagacg ggaaattgga acggaaggtt tcatgggatc
    10381 ccgagaatga aaatggaagg gctttcagcc cgcgaaatat acaggtcggc gcttcaggct
    10441 gcccgcgccg aagcttgtat tgcgcgggaa taagatgaat tctcgcgcgc gtgaattact
    10501 cccgacgggt caacaagtgg gtcgcgctgg ctatcgcgtc gattggcgtg ctggttgcat
    10561 ggttttcgca gcgcgaggcg gggaaatccc cgcccgggac ggcgccggac aacgtggaca
    10621 ttccagcgcc aaaaccggct ggcgccgact tcgactttta tctaatggca ttgaccgcgc
    10681 atcccgcgtt ctgcgccgac ggaaattccc gccggcagga gtgccgcggc ggcggacacc
    10741 ggtcgctggt cattcatggc ctctggcccg agcgcatgga accgcgcacg tatccgcgtg
    10801 attgcccggc gccaccgctc gacctcgagc ccgcactggt ccagcagctc ggcgagctga
    10861 tgccgggcat ggccgatgga ctgcacgtac atgaatggcg cacccatggc ggttgcagcg
    10921 gactcggcga cgacgagtac tacggccgcg cgctcgagct cgctcgcacc gtggatgcgg
    10981 cgctgggcgc caagctaact actctggcgg gtggcgagac cacgccgggc gaactgcgtg
    11041 gcgtcgcgga cctgttcgaa ccgggtctcg ggcgcacgct cactttccac tgccgcacgc
    11101 tgcgcgacgg cggtaaccgc cggccctatc tcttcgaggt gcgtcagtgc gtggacaacg
    11161 acgggcccgg aggtgcgccg cgcaccgcgc tcgtatgcgc caccatgaaa cgccgcgacc
    11221 agggttgcgg cgcctcgttc atgattgccg gagaggccct gcggtgaatt tctccgccca
    11281 actcgtcgaa ctcgccatcg cctacggcgt gaccgcggcc gcgatgctcg ccggccttct
    11341 gctcatgcgc gccgacagtc gcgcgtattt ccgctggcct gtctactcgg ccatggcgat
    11401 ggtgttgggc gtcgtgtcgt ggaacctcgc gcgcaaacac gggttgccgc cggagtgggg
    11461 cgcgacgcat gcgaacgcca tgtattacgg cgccctggcc gtctatgcga ctcttggatt
    11521 cggtctcggc ctgctggtcg gcaggatcac tcaatcgaaa acggagtaac atgctcgcgt
    11581 tttcagcatg gagcgaatca tggccaagtc gaagaagaag accccgtcga agaaagaagt
    11641 gaagaagctg cgcaaggaga tcaacgccgt gaccaaggct ttcctcgcac gatacgacta
    11701 cgacttcctc atggtagggc cgccggggcc gcgtccgccg ccgaagctgg aaatcagcat
    11761 cctcaacctg tcgtcgtact gaactacttc ggcaggtcga gcccggtgaa atagggctcg
    11821 cggcgcgcac gccagacgtt gcgccagcct ttctgccagg cggtgttcag cgccgcctgt
    11881 gcttcaggca tcttgccttg catcgccagc gattcggcgc gcagccggta catgccgaag
    11941 tgtttgccgc cgttctcttc gtagcgatcg agcatgtcgt cgagctcgct cagcagtttc
    12001 agggcgcggt tccggtcgcc gccgcccttg agttcgatac ccgcgagcaa cagcgccacg
    12061 gaatattcgt ggcggatctg gctgccatcg aacatgtccg cgctcgacat cggcaacacg
    12121 cgctcggcat ggacgagcgt ggtacgtgcg gccgccggat cgccggcgac gagctgcatg
    12181 cgggcgagtt cgacgagttc ggcgcccgtg ccggcgcggc tggtcaggtc gcgccccacc
    12241 atccacgcct tgacggcgtc gccgccaccc tctgcgtaga cgatgttcga ttcacgcgcc
    12301 agcgagaaga agctgccgtc cgccggcagt gaacccatga ccaggcgtgc atcggcaaac
    12361 ttcgcttccg ccagcagcat ctcgatcttc tgatcggcta gataggcgtc gaccggctgc
    12421 agcttgcgcg ccgtgtcgat ccactggatg gcttcgtcgg tcttgccctg ggcgcgcgcg
    12481 atccacgagg tggccagcgg cccccacatg tgggtcggat cgagttcgcg cgcgcgtgtg
    12541 cacgcggccg tggcctcggc gtagtcgccg agatcgatga gctcgatgca gcggaacaca
    12601 tgcgagatga aatccatggg atcgagggcc gcggacgcgg aaaaatgcgt gagcgccttg
    12661 ccgggttcgg cgcgccggtc gaacagatcg ccgaggaaac gatggctcga cgcatcgttg
    12721 ggattcagct tggtcgcccg cagcagcagg ggaagcgctt cgtcggagcg gaactcctcg
    12781 ttgagcagcc agcccttgac cgccagcacc tcggccaggt tcgggttgat gtgcatggcg
    12841 cgattgatga gcggctccac ttcggccttc acatcttcta tgggcgcacg gttcagcgag
    12901 agcccgttga gcaacgactc cgacaacccg accagcgccg gtatgtattt cggatccgcg
    12961 ttgacggcgc gccggaagta ttcgatcgac ttgaggttgt cgtccgcggt gcgccggctt
    13021 tgacgcgcgc ggcccagcag gtacagctcg tgggcctcca tgcgctgcgg ctgacgttgc
    13081 gcccagacct cggcggtctc cgcagtcagc tcgaggtgca gcttttcggc cacggcgcgt
    13141 gagacggagt cttcgatccg gaaaatgttg cccatcggat ggtcgaagga atccgaccag
    13201 atgtggaggc ccgtatccgc ggcgatgagc tgcacggtaa tgcgcaactg gtcgccggag
    13261 cgccgcaggc tgccttcgag cacatgcgag gcgcccaggg cttcggcgat cttgcgcacg
    13321 tcgatgttct ggcccttgaa agcgaaggcc gaagtccgcg ccaccacgcg aagggtcgga
    13381 atgtgggcca gccagttcga caactcttcg gtcaggccat cgcacagcgc ctgttcgctc
    13441 tgatccacgc tcatgtcgac cagcggcagc accacgatgg tcggaaatga ctccgtaacg
    13501 acgtccacct gaaacaaatt gcccggcatc cgccacgcga ccagacccag gatcagggcc
    13561 gcgacgacgc tgccgaaccc gagggccagc cgtttgagcg gcgcccgacg gtccgaagcc
    13621 cggcggccga tgaccgcgcc ggtttcgaag gaggtcggga cgtattgagc gggcagggtc
    13681 gggatgccgg ccatgggcag gggtgggtct tcgaggtgcc ccacgaatcg gtagcccttc
    13741 cgcgggatcg tttcgatgta cctgggggta tcggcgtcgt cgccgagggc aatgcgcagc
    13801 ttgcgcatgg cgctgttgag tcccgtatcg aactccacca cgcccgtggg ccataaccgt
    13861 gcaatgagct gttcccgcga gacgacctcg ccggggtgct tcaccagctc atcgagtatc
    13921 tgcaggggtt ggtcttgaag tcgatgggtg ttgccgtccc tcgagatttc tcccgaatcg
    13981 aagttcactt tccatccatc gaaccggatt tcgcgtctgt ccacggctgg atcatagaca
    14041 agtcagcgcc gaatcagcgt ccgtccatcg cgtcggggcc gccgcggagc gaagttccat
    14101 cccgcaaata gcaaaaaagc aaagccaagg aggtcaacaa tgaatgtcac tgcgcatcgc
    14161 agcatcgggc cggctccact gccgggtccc gaaccgcagc ccgatgcccc gggtcagcct
    14221 ggacccaggc ccccgccgaa gcatctttcc gcccgtgcat tgacggcgtt agttagccaa
    14281 ttgaccacgg ggtatcccaa tcctgacagc ccggaggata ctccgggccc cctggacccc
    14341 tacatccgca aggccctgga gcgttccgtc atcggtgcag gcaccagtgg atatctctgg
    14401 cgcgtcatcg cggagaaaca tccggagatc tgggacgtca tcggcggtga tccgcacacc
    14461 caggtggcgc tgaaccccca gccgctgcct cccaagtcgg cgttcctggc cgcggtcatc
    14521 ctcgaattca ccgaacgcat gaccatcgtc gccgagatcg cggacctcat ccccaggcca
    14581 ggcggggagc gcggaatcat cattgttggg ggtcacgtag cccgcttcgt cgatgacatc
    14641 tgtggcaatg gattgcacat caagtggccg ctgccctggc cctcgccccc gtggttcaag
    14701 gacacgctga gtggcgccga cctcatcgtc atgggcacac agtttcaaca gtcggccgtg
    14761 attgcgatgg accgcgatct cggccgcact ttcgccgatg cggcgcacgc gctgctggaa
    14821 gcgggagccg ccagactcgt ttgacggtcg ttttgtggag agggagggaa cggattccct
    14881 caggcccgcc gccacggtgt tctacccccg ggcaccgtgg cgagccggcc acccggcccg
    14941 ggttaactaa ctacctgcgg ttcgggacgc gcctggatgc agacgtattg cttgcgatat
    15001 tcgaagccgc gatcgggcca tgtctgcgcg tagcggacga gcgcggctgc gccttcgggc
    15061 cggtcgacgc cgttcacgcg gcaatcggcc ttgatgagtc cctggtcgtc acgcgatacg
    15121 aagaggcgaa caccgtagaa attctggtcg cgcagaagat cgtcgaatgc ggcgatgctg
    15181 ttggtgaaca ggcagcaggg gcagaaatcg tgatctcccg ccaccacgtc ctggttctgc
    15241 gccatctgca gcgggggtcc gaacacgatg cggcggtcca ccgggagcac ggacgtcgat
    15301 tcagcgcctg gtttcatctc catgaccatg cagtccacgg ccttgggccg ctgcgcatcg
    15361 agaaacaccg gcaggtcgag ttcggcccag ttcttgaagc ccttggcgaa agaatcgcgg
    15421 acgtcggttc ccgacgagtg ctgatactcg aagaggctgc cctgcaaaag cgtcggatgc
    15481 gagacctgga tcgtgctcac ggtcttcacg ccggaattct cgagcggctc gacccccacg
    15541 acctgcggta acagcgagaa atcgcccagc tccacccagt cgcctttcgc cgttgcctcg
    15601 tggccctgcg ctttcagcgc ggccgacagc gacgtcgtca gatcgtcggt ttcctgccag
    15661 gtcttctgcg cgttgctgaa cgcgacgcgc acgatgtggc cctggcccgg atttcccgtg
    15721 gcgggtctcg gcgggcgcgg tgattttcgg aaccaggaga acatggggcc ggaggttaca
    15781 ccggcgcgcg atcgcaagct gctaggcttg gcgcctctgc ccgggggtcg tgcatgtcat
    15841 atttccgcct gtcattcgtc gcgtcgtggg cgttgttcgc gagtgccgcg ccggcaatcg
    15901 ccgcgccgtc cgtttcggtc gcgagtccgg atggccgcat cgtcgtggcg gtctcgctcg
    15961 ccgatgcgcg ggcgacctac accgtgagcg tcgggggcaa ggaaatcctg cggcagtcgc
    16021 gcctcggggt ggtgcgcgac gacgcggact tcaccaccgg catcgatgcc acggctaact
    16081 acgagaagcg tgcggcgaaa ctcgagaagg tcgaggatcg ttatgaactg ctgacctcca
    16141 agcgccggca gaacgtgtat cgcgcgaatc gccgcgtgct cgagttcaag acgccggccg
    16201 gcgcccgcat ggacatcgaa ttccaggtgt cgaatgacgg gtacgcgttc cgctacgtgt
    16261 tcccggagac ggacgcgacg gtgcgcaaga tcagccgcga agcgtcttcg ttcaattttc
    16321 tcgccggcac gcgcggctgg ctgcagccca tcgcgcccgc gcgctcgggc tggaacgaaa
    16381 cgaatccgtc gtacgaggaa tattacgagc gcgacattcc cgcgggtcag ccttcactgc
    16441 agggcggcgc gtgggtgttt ccggcgctgt tccgatcggg cgatacctgg ctactggtga
    16501 gtgaagccgg tctgcggcgc aactactgcg gatcgcgttt gctcgccgcg cggcgcagca
    16561 gcgaatactt catcgatttt ccggggccgc tcgaagccag caacggcggc gccgtgacgc
    16621 cggaatcgac gctgccgtgg atcacgccct ggcgcttcgt cgtcatcggc gatctcaaga
    16681 cgattgccga atcgacattg ggcaccgatc tcgccgatcc gcccgcgaag ggtttcaaga
    16741 ccacggcttc cggccccggc aaatcgtcgt ggagctggcc gttgttaggt gacgaccaga
    16801 cgatcatcaa ggtgcagaag cgcttcgtcg actatgccgc cgagatgggc tggcaataca
    16861 cgctcgtgga ttcggcctgg gaccggcaga tcggttacga cgggttgaaa gagctggtcg
    16921 agtacggccg tccgaagaac gtgaagatcc tgatctggta caactcggcc ggtgagtgga
    16981 acaccacgcc gctgacgccg cgcgaccgca tgcacgaccc ggcgacacgc cgcgccgaga
    17041 tgcagaagat caaggacctc ggcatcgccg gcgtgaaggt ggatttcttc gcgggtgatg
    17101 cgcagtcgac gatcgcgtat taccacgaca tcctcgaaga cgccgcgcgc atcgggctcg
    17161 cggtcaattt tcacggcgcc acactgccac gcggctggca gcgcacgtat ccgaacctca
    17221 tgaccatgga gtcggtgcgc ggcctcgaat tcaacacgtt cgagcagcgc aacgcggaag
    17281 agtcggcgac acacgacgcc atgttgccgt tcgcgcgtaa cgtgttcgac cccatggact
    17341 tcacgcccgt ggtgctcgat cgcctcaaca atgtcgtcga gcggcgcact tcggcggcgt
    17401 tcgaactcgc gaccgcggtg ttgttcacct cgggcatcca gcactacgcc gagattccgc
    17461 aaggcatggc gaaggcgccg gtctacgtgc gcgatttcct caagaaagtg ccggcggtgt
    17521 gggatgacgt caggttcatc gacggattcc cgggccagta cgcggtgttc gcgcggcgcg
    17581 cgggcaacac ctggtatgtc gccggcatca atgccgaccg gcaacagccg cgcaaggtga
    17641 agatcgatct gaaagcgctc ggcgtcgcga agcagggcac gctcatcacc gacggcatcg
    17701 actttctcgg gttcaagagc gagacgtttc cgctcgagaa gggcgcgacg tcggacgaga
    17761 tcaccatgcg tccgcgtggc gggttcgtgc tcaccttcaa ttaaaaacac agaaaaacag
    17821 ccaggaaaac gttggctttg acatgtgacc aaaaggtcac atatagtccg tgcatggtcg
    17881 acgaagatct ggtgttcaag gcattggcgg attcatcgcg caggttcctg ctcgatctcc
    17941 tgcacaagcg tgacggccag actttgggcg agctcgagtg ggaactcgaa atgacccgct
    18001 ttggggtcat gaagcacctg aaggtgctcg aagaggcggg gctcattgtc acgcgccgcg
    18061 aagggcgcga gaagctccac ttcctcaacg ccgttcccat ccggctcatt cacgaccgct
    18121 ggatcgacaa atacaaggag cgcgaactgt ccgcgctggc tgacctcaaa tccactttgg
    18181 agaaaacgaa atgccccccg ttgcgcaagc gagcctgaag cctgaagtga actcgaccac
    18241 ccacatccac cagatctaca tcaaggcgga tccggcacgg atctgggatg ccatcacgag
    18301 tcccgagtgg aatgggaaat acggctacgg tggcgtcagc cactacgacc tcaagccggg
    18361 tggaaaattc aaatgcatcg ccaatgagca gatgcgttcg ttcggcctgc cggaagtcat
    18421 cgtcgacggc gaagtcatcg aggtgaagcc gccgtacaag ctcgtgcaga cctatcgctt
    18481 cctgttcaac gaggaacaca agaaggaagg cttcacccgt atctcgttcg agatcgagca
    18541 gacttcgggc ggcttctgcc ggctcaccat cacgcacgac accgcgggcg cgccgatgat
    18601 ggccggagcc acgcagagca acttcagcca agagggtggc ggtggatggg actggatcct
    18661 cagcgacctc aagtcgctgc tggagaccgg caagacgatg tccgactgaa gctcgagccg
    18721 gttcagtgcg ggagtggaga ggcgaagctt ctctactccc gcgcgaactc gttgctcaat
    18781 cgagcataat gctgcgcctg cgggcgtatg catgaaaacc ctacgactga aagtgaagcc
    18841 cggctcgcgc gaagaggggc tggaagagct ggatgacgga agctggcttg cgcgcgtcaa
    18901 ggcgccgccg gtagatggga aggccaacac ggccgtcatt gcgctggttg ccaggcactt
    18961 cgggcttcgc aaggcccagg tgaacatcaa gagcggcgct tccggccgca tgaaactcgt
    19021 tgtgctcgag gactgaccag catgatcacg ctctacacct tcggtcccgc tttcggactg
    19081 cccgatccca gcccattcgt catgaagggc gagatgttgc tcaagctcgc gaagctcgac
    19141 taccagccag acaccaaggg atttctgcgc gcaccgaagg gcaaactgcc gttcatcgac
    19201 gacaacggca ccatcgttgc ggattccacg ctgattcgcc tgcacctcga acagaagcac
    19261 ggcgtcgact tcgaccgcgg attgtcgatg cgtgaccggg gcatcgcctg ggcgacggag
    19321 aagatgctcg aagaccacct gtactgggtg ctggtgtact ggcgatggat gaaggacgac
    19381 aatttcgagc gcggcccggc taacttcttc aagcgtgcgc ccgcgttcat ccgcccgctc
    19441 gtcaagtgga aaattcgcgg caacgttcgg cgcagcctgc acgcccacgg aatcagccgg
    19501 cacaacgaga ccgagatgac cgccatgtcg gaccgcgcct tcgaagcgct ctcgcgggtg
    19561 ctcggcgaaa acaaatacct catgggaaac gaaccttgcg gcgccgatgc caccgcattc
    19621 gcgttcgtcg ccggcgcgct gacgccggtg ttcgagtccc ccgcgcacgg caaggcgcgc
    19681 acgctgccga acctgatcgc ttaccgggac cgaatgatgt ccgagttcta cccggggttt
    19741 aaatattaga cgcgcctcaa ctcgaggagt tggcatgaaa ctggttcgac tgatagccac
    19801 cgcgctcttc gcgctgcaga tttcgctcgc atcgtttgcc gacggctccg gctccgccac
    19861 gcgcgccgcg ttcctgcagg tcatcgaccg gccgcgcgtg gcattgaagg ccgaactcgc
    19921 cgagatggcg ccggtcgagg gcttccggaa ataccacctg
//
LOCUS       DATDEU010000096         2189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1039511, whole genome shotgun sequence.
ACCESSION   DATDEU010000096 DATDEU010000000
VERSION     DATDEU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039511"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /gene="gshB"
                     /locus_tag="VIV63_02265"
     CDS             <1..661
                     /gene="gshB"
                     /locus_tag="VIV63_02265"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY6123448.1"
                     /translation="ILERAEIQGALVANRPQGLRDMNEKVFTAWFPQCCAPTLITRSA
                     SDITAFAGEHGKIVVKPLDGMGGRSIFVTEKGDKNLPVIIETLTDYGQRFAIAQRYVP
                     DIATTGDSRVLLIDGQPVPYALARIPLASDNRGNLAAGATGVGRELNERDRWLAAEIG
                     PALSKLGMVFVGIDVIGGFVTEINVTSPTGIRELDKQFGLNIGDMVIAALERRIGARK
                     A"
     gene            663..1574
                     /locus_tag="VIV63_02270"
     CDS             663..1574
                     /locus_tag="VIV63_02270"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6123449.1"
                     /translation="MSARAMQLREGIAPTRDRLTTTVFMAALVHGVIIVGVTFGGVKH
                     DKPGAPGLEVLIVSEDVPEARRNDDATYLAQRTQLGSGTTEPGEVTRAPRAAAEHAAR
                     NGRNDGTVLAEDAGALLATADSRSLTTTAPMPEILYFAPPASMTEMTEVPVQTEGNSE
                     RDKLGIDDDEEQKVRGPQRDELWVTPDTREALVAPYLVSWRLKIERLGTMNFPQAAWR
                     APGTRNPDVEVSIFADGTLESATIVRSSGSAKLDQAVVDILKLASPFDPFPKELAEKH
                     RVLRFKYGWEFQNPNRHGAVTAPADSR"
     gene            1585..2151
                     /locus_tag="VIV63_02275"
     CDS             1585..2151
                     /locus_tag="VIV63_02275"
                     /inference="COORDINATES: protein motif:HMM:NF001266.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY6123450.1"
                     /translation="MSGGPTSLSNHLLIAMPQLADPNFSQTVSLICEHGEKGALGIVL
                     NRPLTMTLSEVFEQMKIEPTDKRAADLPVLRGGPVHQDRGFVLHRPGGEWDSTHKISD
                     SIQVTTSRDVLAAMATGTGPDKAFIALGYAGWEAGQLEKEMLDNSWLSVPADESVIFD
                     LPYEERWLATLRLLGVEPAQLTSFSGHA"
BASE COUNT          412 a          758 c          670 g          349 t
ORIGIN      
        1 cattctcgag cgcgcggaga tccagggtgc gctcgtcgcc aaccggccgc agggcctgcg
       61 cgacatgaac gagaaagttt tcacggcgtg gtttccgcag tgctgcgcgc cgacgctgat
      121 cacgcgctcc gcctcggaca tcaccgcgtt cgcgggcgag cacggcaaga tcgtcgtgaa
      181 gccgctcgac ggcatgggcg gcaggtccat tttcgtcacc gagaagggcg acaagaacct
      241 gccggtcatc atcgaaacgt tgaccgacta cgggcagcgg ttcgccatcg cgcagcgcta
      301 cgtgcccgac atcgccacca ccggcgattc gcgcgtgctg ctcatcgacg gccagccagt
      361 cccttacgcg cttgcacgta tcccgctggc ttccgacaac cgcggcaatc tcgccgccgg
      421 cgccaccggc gtgggacgcg agctcaacga gcgggatcgc tggctggcgg ccgagatcgg
      481 gcctgcgctc tcgaagctgg gaatggtctt cgtcggcatc gacgtcatcg gcggtttcgt
      541 taccgagatc aacgtcacga gtccgacagg aattcgtgaa ctcgacaagc aattcgggct
      601 caatattgga gatatggtaa ttgccgccct cgagcgccgc ataggcgccc gcaaagcttg
      661 aaatgagcgc ccgcgcgatg caattgcgcg aagggatcgc gcccacgcgc gaccggctca
      721 ccacgactgt cttcatggcg gcgctggtgc acggcgtcat catcgtcggc gtcaccttcg
      781 gcggcgtgaa gcacgacaaa cccggtgccc cgggcctgga agtgctcatc gtctccgaag
      841 acgtcccgga agcgcgccgc aacgacgacg ccacctacct ggcgcagcgc acccagctcg
      901 gctcgggtac caccgaaccc ggcgaggtca cgcgcgcgcc gcgtgcggcc gccgaacacg
      961 ctgcccggaa cgggcgcaac gatggcaccg tcctcgccga agacgccggc gcgctgctcg
     1021 ccaccgccga cagccgctcg ctaactacca ccgcgccgat gcccgaaatc ctctatttcg
     1081 cgccgcctgc gagcatgacc gagatgaccg aggtgccggt gcagaccgaa ggcaacagcg
     1141 aacgcgacaa gctgggcatc gacgacgacg aagaacagaa ggtgcgcggc ccccagcgcg
     1201 acgaattgtg ggtaacgcct gacacccgcg aggccctcgt ggcgccctac ctcgtctcct
     1261 ggcgcctgaa gatcgagcgc ctgggcacca tgaacttccc gcaggccgcc tggcgcgcac
     1321 cgggaacgcg caatccggac gtcgaggtct ccatctttgc cgatggcacg ctcgaatccg
     1381 ccacgatcgt gcgctccagc ggttcggcca agctcgacca ggcggtcgtg gacatcctca
     1441 aactcgcgag cccgttcgat ccgttcccga aggagctggc cgagaaacac cgggtgttgc
     1501 gtttcaaata cggctgggag ttccagaacc cgaaccgtca cggcgcggtt accgcgcccg
     1561 ccgattcacg gtaaagtgcg ccgcatgagc ggcggtccca caagcctttc gaatcatctg
     1621 ctgatcgcga tgccgcagct cgccgatccg aatttctcgc agaccgtttc gctcatctgc
     1681 gagcacggcg aaaaaggcgc cctcggcatc gtgctgaatc gtccgctcac catgacgctc
     1741 agcgaagtgt tcgagcagat gaagatcgaa cccaccgaca agcgcgctgc cgaccttccg
     1801 gtgttgcgcg gcggtccggt ccatcaggat cgcggcttcg tgctgcatcg tcccggtggc
     1861 gagtgggact ccacacacaa gatctccgat tcgatccagg tcaccacgtc gcgcgacgtg
     1921 ctcgccgcca tggcgacggg caccggcccc gataaagcct tcatcgccct ggggtacgcg
     1981 ggctgggaag ccggccagct cgaaaaggaa atgctcgaca actcctggtt gtcggtgccc
     2041 gccgatgaaa gtgtgatctt cgatctgcct tacgaggagc gctggctcgc gacgctccgg
     2101 ttgctcggtg tcgaacccgc gcagctcacc tcattctccg gtcatgcctg accaggcaac
     2161 ccaagaacca aaaatcgtcg tcgcaatcg
//
LOCUS       DATDEU010000097         4212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1331165, whole genome shotgun sequence.
ACCESSION   DATDEU010000097 DATDEU010000000
VERSION     DATDEU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331165"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VIV63_02280"
     CDS             <1..346
                     /locus_tag="VIV63_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123451.1"
                     /translation="FVRTIAVARVVMPKAFVRLSAGREAMVDELQALCFLAGANSIFY
                     GEKLLTTGNPDVARDRALLTRLGIEPLVPPALAAAHASEPAGHVHGPECSHAASHVAP
                     RPSGSCNTTRQR"
     gene            277..1470
                     /gene="bioF"
                     /locus_tag="VIV63_02285"
     CDS             277..1470
                     /gene="bioF"
                     /locus_tag="VIV63_02285"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY6123452.1"
                     /translation="MFTRGFARGAAPEWLVQHDPAALKSALADLEQRNLRRTRRVGQR
                     SGVINFCSNDYLGLAKHPAVVGAFQDAATKYGVGSGASHLVTGHGPEHEALEEALAAF
                     TGRAKALVFSTGYMANMGVISALADQNAVLVSDKLNHASLIDGCRLSGAESLRYRHGE
                     STHAFELLAAVDPAIDTRLLITDGVFSMDGDLAPLPDLARAARQTGAWLMVDDAHGFG
                     VVGATGRGTCEHFGLNAGDVPVLVGTFGKAFGTFGAFVAGDADLIEFLMQKSRTYIYT
                     TALPPALAAATRVALEVSQRESWRREKALALVQRLRNGLAEYFPELRQSVPGTTIVPL
                     VLGDAKRALTVSKALETKGFLVTAIRPPTVPAGTSRLRITLSAAHEESQVDGLIVALR
                     ELIAA"
     gene            1467..2366
                     /gene="bioC"
                     /locus_tag="VIV63_02290"
     CDS             1467..2366
                     /gene="bioC"
                     /locus_tag="VIV63_02290"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEY6123453.1"
                     /translation="MSRPDPTQVFSLDRRAVLQAFDRASGSYDAAAALQERVRNDLLG
                     RLTELKVEPRTVLDLGAGTGHSSRALKRRYPRALVVAADIAPGMLARARAQSRWLRRF
                     ERVRADAYSLPFRDASFDLIYSSLMLQWCDDLDAAFGEIARVLKPGGLLLFSTFGPGT
                     LAELREAWGASDATGNHVNHFFDPQALGSALMHAGLAEPVLDVDRIVMRYANVLTLMR
                     ELKAIGAHNVTQGRPRGLTGRKRLAAMTQAYEVLRHDGELPATYEVINAICWGSERRA
                     QPHDDFPRETFIAPAAIRRRVQP"
     gene            2363..3067
                     /gene="bioD"
                     /locus_tag="VIV63_02295"
     CDS             2363..3067
                     /gene="bioD"
                     /locus_tag="VIV63_02295"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY6123454.1"
                     /translation="MTPGTAKQGFFITGTDTGVGKTLVTVALTRALVARGIRTAVMKP
                     VAAGIVQTPDGPRNDDALELLAASNVSAPYEDVNPWLLTTPASPHLAARHDGVSINHE
                     RVLAAYRRLAARSDLVLVEGAGGWLAPTSSIATMADIAEKLELPVIFVVGLRLGCLNH
                     ALLTREAIRSCGLPFAGWIANKMAVEMPLAKANIETLTSRFGMAPLAIVPPGAAPDAG
                     AAPAWADEAAKKLVSA"
     gene            3102..3605
                     /locus_tag="VIV63_02300"
     CDS             3102..3605
                     /locus_tag="VIV63_02300"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEY6123455.1"
                     /translation="MFDAATHYDLTPHCSLSVRGAVVFFASVAFATLGIAGVATALGY
                     WPVLPFAGAEMALLGWALYSNMQRRHEHETIDISETEVVITYSRGNPSRVVFPRHWAR
                     VKIRRPYSPLHRGQLVIESHGRAHEVGQFLTEEERRQLAAALRLRIGGMNQSPALPAA
                     GSPDQFS"
     gene            3626..>4212
                     /locus_tag="VIV63_02305"
     CDS             3626..>4212
                     /locus_tag="VIV63_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEY6123456.1"
                     /translation="MKNAYARRGLQAAALSLFALLASVAHADWGGLNMTEGVSILSKE
                     IYGLHMEIFYWCVGIGVVVFGVMIYSLVKFRKSQGATPDTTLVHSTKVEIIWTVIPIL
                     ILVAMAVPTARGLIKIENMEGATIDIKVTGYQWKWEYEYLGQNVSFFSTLKRDSNVAR
                     QLGSGVDPKSVPNYLLDVDNPLVIPAGTKIRFLLTA"
BASE COUNT          702 a         1435 c         1347 g          728 t
ORIGIN      
        1 cttcgtgcgc acgatcgccg tcgcgcgcgt ggtgatgccg aaagccttcg ttcgtctatc
       61 ggctggccgc gaagcgatgg tggacgagct gcaggccctg tgcttcctgg ccggcgcgaa
      121 ctcgatcttt tacggcgaga aactgctgac cacggggaat cccgatgtcg cccgcgatcg
      181 tgcgctgctg acgcgcctcg gaatcgagcc gctggtgccg ccggcgctgg ccgcggcgca
      241 cgcgagtgaa ccggccgggc acgtacatgg ccccgagtgt tcacacgcgg cttcgcacgt
      301 ggcgccgcgc ccgagtggct cgtgcaacac gacccggcag cgctgaagtc ggcgctcgcc
      361 gatctcgagc agcgcaacct gcgccgcacg cggcgcgtgg gccagcgttc gggcgtcatc
      421 aacttctgca gtaacgacta cctcggcctc gccaaacatc cggccgtggt cggagctttc
      481 caggacgcgg ccacgaaata cggcgtgggc agcggagcgt cgcacctcgt cacgggccat
      541 gggccggaac acgaggcgct cgaagaggcg ctcgccgcct ttaccggccg tgcgaaggcg
      601 ctggttttct ccacgggcta catggccaac atgggcgtca ttagcgcact ggcggatcaa
      661 aacgccgttc tggtctcgga caagctcaat catgcgtcat tgatcgacgg ctgccgcctg
      721 agcggcgccg agtcgctgcg ttaccgccac ggtgaatcga cgcatgcatt cgagctgctc
      781 gcggcggtgg atccggccat cgatacgcgg ctgctcatta ccgacggcgt gttcagcatg
      841 gacggggacc tggcgccgtt gccggatctc gcgcgggccg cgcgacagac cggcgcatgg
      901 ctcatggtcg acgacgcgca tgggttcggc gtcgtgggcg ccaccggccg cggcacctgc
      961 gaacacttcg gtctgaacgc cggtgacgtg cccgtactgg tgggcacctt cggcaaggcc
     1021 ttcggcacct tcggcgcgtt cgtggcggga gatgccgacc tcatcgaatt cctcatgcag
     1081 aaatcgcgca cgtatatcta tacgacggcg ttgccgccgg ccctcgccgc agccactcgt
     1141 gtggcactcg aggtgagcca gcgcgaatcg tggcgacgcg aaaaagcact ggcgctggtg
     1201 cagcggcttc gtaacgggct cgccgaatac tttcccgaac tacgacaatc ggtgcccggc
     1261 accaccatcg ttccgctggt gctcggtgat gcgaaacgcg cgctcaccgt cagcaaagcg
     1321 ctcgaaacca agggtttcct cgtcaccgcg atccggccgc cgacggttcc cgcgggtact
     1381 tcgcgattgc gcatcacgct gtccgcggca catgaagagt cgcaggtgga tggattgatc
     1441 gtggcgctgc gcgagctgat cgcggcatga gccggcctga cccgacccag gtgttctcgc
     1501 tcgatcgccg cgcggtgttg caggcgttcg atcgcgcgag cgggagctac gacgcggcgg
     1561 ccgcgctgca ggaacgcgtg cgcaacgacc tgctgggccg gctcaccgaa ctgaaagtgg
     1621 agccacgaac cgtgctcgat ctgggcgcgg gcaccggcca ttcctctcgc gcgctcaaac
     1681 gtcgctaccc gcgtgccctg gtcgtggccg ccgacatcgc tccggggatg cttgctcgcg
     1741 cccgggcgca gtcgcgctgg ctgcgccgct tcgaacgcgt gcgcgccgat gcgtattcac
     1801 tgccctttcg cgacgcgtcc ttcgatctca tctacagcag cctgatgctg cagtggtgcg
     1861 acgatctcga cgccgcgttc ggcgagatcg cgcgcgtgct caaacccggc ggcctgctgc
     1921 tattcagcac tttcggtccc ggcacactcg ccgaactgcg cgaggcctgg ggcgcgagcg
     1981 acgccaccgg caatcacgtg aatcacttct tcgacccgca ggcgctcggc agtgcgttga
     2041 tgcacgcggg gctcgccgag cccgtgctcg acgtcgaccg gatcgtcatg cgttacgcga
     2101 acgtgctgac gctgatgcgc gagctcaagg cgatcggagc acacaacgtc acgcagggcc
     2161 gtccgcgcgg actcaccggc cgcaagcggc tggccgcgat gacccaggcg tatgaagtcc
     2221 tgcgccacga cggcgagctg cccgccacgt acgaagtcat caacgcgatc tgctggggca
     2281 gcgaacggcg cgcccagcca cacgatgatt ttccgcgcga gactttcatc gcccccgccg
     2341 cgattcgccg gcgcgtgcaa ccgtgacgcc cggtacggca aaacaaggct ttttcatcac
     2401 gggcaccgat accggcgtcg gcaagacgct ggtgaccgtg gcgctgacgc gcgcgctggt
     2461 ggcgcgcggc atacgcaccg cggtcatgaa acccgtcgcc gcgggcatcg tgcagacacc
     2521 cgacgggccg cgcaacgacg acgcactcga gttgttagcc gcgagcaatg tcagcgcgcc
     2581 ctatgaagac gtgaacccgt ggctgctgac cacgccggca tcgccgcatc tcgccgcgcg
     2641 tcacgacgga gtctcgatca accacgaacg agtgcttgcc gcgtaccgtc gcctggcggc
     2701 gcgttcggac ctcgtgctgg tcgagggcgc cggcggctgg ctcgcgccca cctcgagcat
     2761 cgcaaccatg gcggacatcg ccgaaaagct cgaactgccc gtgatcttcg tcgtgggcct
     2821 gcggctcggt tgtctcaacc acgcgctgct gacgcgcgag gcgatccgtt cctgcggctt
     2881 gcccttcgcc ggctggatcg ccaacaaaat ggcggtggaa atgccgctgg ccaaagccaa
     2941 catcgagacg ctgacttccc gcttcggaat ggccccgctg gccatcgtgc ctccgggtgc
     3001 agcccctgat gcaggggcag cccccgcatg ggcggacgag gccgcaaaaa agctcgtaag
     3061 tgcttgagta agccagcaaa gtcgctggta tagtcgccgc cgtgtttgat gccgcgaccc
     3121 actacgacct gaccccgcac tgctcgctca gtgttcgcgg cgccgtcgtg ttcttcgcga
     3181 gcgtcgcgtt cgccactctg ggcatcgccg gtgtggccac cgcgcttggc tactggccgg
     3241 ttctgccgtt cgcgggcgcc gaaatggcgc tgctcggctg ggctctgtac tcgaatatgc
     3301 aaagacggca cgagcacgag accatcgaca tttcggaaac cgaagtggtc atcacatact
     3361 cgcgcggcaa cccgtcacgt gtcgtgttcc cgcgccactg ggcacgggtt aaaatccgcc
     3421 gcccttattc gcccctccac cgcggccagc tcgtcatcga gtcgcacggc cgggcacatg
     3481 aggtcggcca gtttctgaca gaagaagagc gtcgccagct ggcagccgct ttgcggttgc
     3541 ggattggcgg catgaatcag tcgccggcat tgcccgcggc cggttcaccg gatcagtttt
     3601 cttgaggtca tcgatgagct ttcacatgaa gaacgcctac gcgcgccgtg gattacaggc
     3661 cgccgccctc tccctgttcg ccttgctggc gtccgttgcg cacgccgact ggggcgggct
     3721 caacatgacc gagggtgtct ccatcctcag caaggagatc tacggtctgc acatggagat
     3781 cttctactgg tgcgtaggca tcggcgtggt ggtcttcggc gtgatgatct actcgctggt
     3841 caagttccgt aagtcgcagg gcgccacgcc cgacacgacg ctggtccaca gcaccaaggt
     3901 cgaaatcatc tggacggtga tccccatcct gatcctcgtg gccatggccg tgcccaccgc
     3961 gcgtggcctc atcaagatcg agaacatgga aggcgccacg atcgacatca aggtcacggg
     4021 ctaccagtgg aagtgggaat acgaatacct cggccagaac gtcagcttct tctcgacgct
     4081 caagcgcgac agcaacgtcg cgcgccagct cggttccggc gtcgacccga agagcgttcc
     4141 taactacctg ctcgacgtgg acaacccgct ggtcatcccc gccggcacca agatccgctt
     4201 cctgctcacc gc
//
LOCUS       DATDEU010000098         2889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1040080, whole genome shotgun sequence.
ACCESSION   DATDEU010000098 DATDEU010000000
VERSION     DATDEU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040080"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..2475)
                     /gene="lon"
                     /locus_tag="VIV63_02310"
     CDS             complement(10..2475)
                     /gene="lon"
                     /locus_tag="VIV63_02310"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY6123457.1"
                     /translation="MTEPASTSKSVSIQGLPVLPLRDVVVYPHMVIPLFVGREKSIHA
                     LDVAMKADKRIMLIAQKQPDVDDPKTDDLYRIGTVASILQLLKLPDGTVKVLVEGVDR
                     ARIEAMHTGEHYTSDVTTEPDADTYDEKELDVLARSVISQFEQYVKLNKKVPPEVLTA
                     LAGIEQPGRLADTVAAHMALKLPEKQKVLEILDVRKRLEHVLVAIEGEMDVLQIEKRI
                     RGRVKAQMEKSQREYYLNEQMKAIQKELGEMEEGGNELSDLENRIKKAGMPKEALEKA
                     NAELNKLKMMSPMSAEATVVRNYIDWLVKVPWKKRTKILKDIAKAEKVLEEDHFGLEK
                     VKERIVEYLAVQQRVDKIKGPILCLVGPPGVGKTSLGQSIARATNRKFVRMSLGGVRD
                     EAEIRGHRRTYIGSMPGKIVQNMAKTAVHNPLFLLDEIDKMSMDFRGDPSSALLEVLD
                     PEQNSTFADHYLEVDLDLSQVMFVCTANSLNIPAPLLDRMEVIRLPGYTEDEKLNIAK
                     KYLLPKQVKQNGLAPEELKCTEDAILDIIRYYTREAGVRNLERELARICRKAVKQLLM
                     AAKKAEKSEKAEKASDKQVSVSSKNLDKFLGVRRFRYGKAEEENRIGHVTGLAWTEVG
                     GELLTIEAAVVPGKGKLQHTGQLGEVMQESIQAAMTVVRSRANVLGIDPDFYQKHDVH
                     LHMPEGATPKDGPSAGIGMCTALVSALTKIPVRSDVAMTGEITLRGEVLPIGGLKEKL
                     LAAHRGGITTVMIPVDNEKDLTEIPDNIKGKLDIRPVKWIDEVFEIALASQPTPRLAT
                     PPPVPEKRTRRTGKRATKQVQAH"
     gene            complement(2706..>2889)
                     /locus_tag="VIV63_02315"
     CDS             complement(2706..>2889)
                     /locus_tag="VIV63_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123458.1"
                     /translation="ILENVLLDTMYDLPGMKNVSKVVVDDTVIEGHQKPYIVYKSDTA
                     TTDDAAPRRVSGAGLS"
BASE COUNT          469 a          868 c          897 g          655 t
ORIGIN      
        1 gccctcagtc tagtgagcct gaacctgttt cgtcgcgcgc ttgcccgtac ggcgtgtgcg
       61 cttttccggc accggaggcg gcgtcgccag gcgcggcgtc ggctggctgg cgagcgcgat
      121 ctcgaatacc tcgtcgatcc atttgacggg gcggatgtcg agcttgccct tgatgttgtc
      181 ggggatctcg gtgaggtcct tttcgttgtc gaccgggatc atgaccgtgg tgatgccacc
      241 gcgatgtgcc gcgagcaact tctccttgag gcccccgatg ggcaggacct cgccacgcag
      301 ggtgatttca cccgtcatgg ccacatccga tcgcaccgga atcttggtga gtgccgacac
      361 gagcgccgtg cacatgccga taccggcgga cgggccgtct ttcggcgtcg cgccttcggg
      421 catgtgcaga tgcacgtcgt gcttctggta gaagtccggg tcgatgccga gcacgttggc
      481 gcggcttcgc accaccgtca tcgcggcctg gatcgactcc tgcatgactt cgccgagctg
      541 accggtgtgc tgcagcttgc ccttgccggg cactaccgcc gcttcgatgg tcagcaactc
      601 gccgccgact tcggtccagg cgaggccggt gacgtggccg atgcggtttt cctcttcggc
      661 cttgccgtaa cggaaacgac gcacgcccag gaacttgtcg agattcttcg acgagacgga
      721 aacctgcttg tcggacgctt tctcggcctt ctccgatttc tccgccttct tcgcggccat
      781 cagcagttgc ttgacggcct tgcggcagat acgcgcgagt tcacgctcaa ggttacgcac
      841 gccggcttcg cgcgtgtagt aacgaatgat gtcgaggatc gcgtcttccg tgcacttgag
      901 ctcttcgggc gcaaggccgt tctgcttcac ctgtttcggc agcaaatatt tcttcgcgat
      961 gttgagcttc tcgtcttcgg tataacccgg cagacgaatc acttccatgc gatcgagcaa
     1021 cggcgccgga atgttcaggc tgttcgccgt gcagacgaac atcacctgcg acaggtcgag
     1081 gtcgacctcc agatagtggt cggcgaaagt ggagttctgc tcggggtcga gcacttcgag
     1141 caacgccgag gacggatcgc cacggaagtc catcgacatc ttgtcgatct cgtcgagcag
     1201 gaacagcgga ttgtgcacgg cggtcttcgc catgttctgc acgatcttgc cgggcatgga
     1261 tccgatgtac gtgcggcgat ggccgcggat ctcggcctcg tcacgcacgc cgcccagcga
     1321 catgcgcacg aacttgcgat tcgtggcgcg cgcgatggac tggcctaacg aagtcttgcc
     1381 gacgccagga ggaccgacga gacacaggat gggacccttg atcttgtcga cgcgctgctg
     1441 gacggccagg tactcgacga tgcgttcctt gaccttctcc agcccaaagt gatcttcctc
     1501 gagcaccttt tcggctttcg cgatgtcttt gaggatcttg gtgcgtttct tccacggcac
     1561 cttcaccagc cagtcgatgt aattgcgcac cacggtggct tccgccgaca tgggcgacat
     1621 catcttgagc ttgttgagct cggcgttggc cttctcgagc gcctctttcg gcatgcccgc
     1681 tttcttgatg cggttctcga ggtcggagag ttcgttgccg ccctcttcca tctcgccgag
     1741 ttccttctga atggccttca tctgctcatt caggtagtac tcgcgctggc tcttttccat
     1801 ctgcgccttc acgcggccgc ggatgcgctt ctcgatctgc aacacgtcca tctcgccttc
     1861 gatggcgacg agcacgtgct cgaggcgctt gcgcacgtcg aggatttcga gaaccttctg
     1921 cttctcgggt agtttgagcg ccatgtgcgc ggcgacggtg tccgcgagac ggcccggttg
     1981 ttcgatgccg gcgagcgcgg tcagcacttc tggcggcacc ttcttgttga gcttcacgta
     2041 ctgctcgaac tgcgagatca ccgaacgcgc cagcacgtcg agctctttct cgtcgtaggt
     2101 gtcggcgtcg ggctcggtgg tcacgtcgga cgtgtagtgc tcgccggtgt gcatcgcttc
     2161 gatgcgcgcg cggtcgacgc cttcgacgag caccttgacg gtaccgtcgg gtagtttgag
     2221 cagctgcagg atcgacgcga ccgtgccgat gcgatagagg tcatcggttt tcggatcgtc
     2281 gacgtcaggc tgtttctgcg cgatcagcat gatgcgcttg tcggccttca tcgcgacgtc
     2341 gagcgcgtgg atggattttt cgcgtcccac gaacagcggg atgaccatgt gcggatacac
     2401 cactacgtcg cgtagcggca gcaccggtaa tccctggatg gagacggact tggaagtcga
     2461 ggcgggttca gtcacgcgtg attcctcaac gcaccggcga agccgaaaaa ttcagcacgc
     2521 cgtggctgta tttagtcaaa gtgtagcgcg ggagatgggg ctgcgcgggg gcgtttcaac
     2581 gcttgcgacg cagttcccga ttcgccggta ggcgaaacgg cagaaaaatg ccggtgtgga
     2641 aacaccggca tgtgtggaat cacttagctg attgcctgtc ggccgaaact gcgtgagcgt
     2701 gcggattagc tcaaacccgc accggacacg cggcgcggcg ccgcatcgtc ggtggttgcc
     2761 gtgtcggact tgtagacgat gtaaggcttt tgatggcctt cgatcaccgt gtcgtccacc
     2821 acgaccttcg agacgttttt catgcccggc aagtcgtaca tggtgtcgag cagcacgttt
     2881 tcgagaatg
//
LOCUS       DATDEU010000099         6748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_893799, whole genome shotgun sequence.
ACCESSION   DATDEU010000099 DATDEU010000000
VERSION     DATDEU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893799"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..498
                     /locus_tag="VIV63_02320"
     CDS             112..498
                     /locus_tag="VIV63_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6123459.1"
                     /translation="MITHSGGCHCSRVRFEVDAPAELSIDDCNCSMCSRTGYLHLIVT
                     KDRFRITKGANALTTYTFNTGTAKHLFCATCGIKPFYVPRSHPDGFSVNARCIDSDTV
                     KGMKINPIDGRNWEQQYPAGRGEFTA"
     gene            509..865
                     /locus_tag="VIV63_02325"
     CDS             509..865
                     /locus_tag="VIV63_02325"
                     /inference="COORDINATES: protein motif:HMM:NF023903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HEY6123460.1"
                     /translation="MSHFADNIRPYVDAELRAAEQDAAREFAHLERAHVLGQASTREH
                     VRVHWRMLKWAWRHRDAREWFGQVFRILGAATKTFIGLVPAGNTGGANVSPVRPMPLD
                     PELAAIIARARKTDPR"
     gene            complement(866..1657)
                     /locus_tag="VIV63_02330"
     CDS             complement(866..1657)
                     /locus_tag="VIV63_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY6123461.1"
                     /translation="MRIERIWAANSLRNFHYLIACEETGEALIVDPLDAAQCLAAARA
                     AGLSITQILNTHEHRDHTDGNAGVVAATRARVNAHAGAAGHIGGVDRGLARGDVIKVG
                     RTVELEVLDTPGHTRSHVCLLAHGETPALFCGDTLFNAGAGNCHNGGDPGLLYETFVN
                     QLARLPDATRVYPGHEYMARNLAFTLDREPGNVDAAKALESAKSQRPEDARVTTLGEE
                     KRVNAFLRLQNPQLVARLREKFPEIGEKPDARTVFVKLRELRNSW"
     gene            complement(1667..3715)
                     /locus_tag="VIV63_02335"
     CDS             complement(1667..3715)
                     /locus_tag="VIV63_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEY6123462.1"
                     /translation="MNRLLRPFGLKLGPLQAGVIYFPLHFFAVGIGLTLLTGSQGVGL
                     FWPAAGSLFAFLLLYPARFWPALFVTCYAAELLAHTVFAAQLPFSAAGLLFPVKFSAA
                     LVGVGVMHAAVRGPISFARLRHVLFFAVAALVSTLLCAVVAIALRGDESFGDLAYWQG
                     VQGWWIGDFLGALIVTPLLLTVGFHGFALSKDSRGGRAATLSAFAVLLLMLAFVFLRK
                     AGTLMSPLDVPYIVYPVLVWIAMLSGPRRTALAAGLTVAIASLATTRGYGPFDSAYQL
                     QTFLALCVLPVLLLQAVMSERDSAIAQARASDERYRAFIANSSEAIFRTELAEPMPIT
                     LPPDEQVAWVRKHGFVAECNTAFMVALGVKDAQSPIVGTRLGDHPTWSKIYIERIREA
                     IRNGYQVRNIEHVVHGPYGLDRVLLISMIGIVENGMVLRFWGSGRDVTAMREAEAALA
                     QHDSQLRALATEITLAEERARRKLASELHDGPAQNLMGLGMQLAEMKRGLNDRAQLQR
                     IDEAEQVLADATLQTRTLMLELAPPGLHESGLLEALRWLADRVSKQQRLMVTVEDDGN
                     PKPLEDQVTVLLFQTVRELLQNVVKHARSKRATVRCSVNDDSLALDVIDPGVGFEVHS
                     INRLPTRQGGFGLFNIRERLKLMGGSIDIHSIVGEGTTVRIRVPLKSQHGLFDEALRA
                     "
     gene            3867..5441
                     /locus_tag="VIV63_02340"
     CDS             3867..5441
                     /locus_tag="VIV63_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY6123463.1"
                     /translation="MLFPLLGTLAACGGGGGGGSSSSSSSSSSGGSTWQPGVYLPSSN
                     FDAMCAAPRAGTADRRGSVTDQNNWLRSWTNELYLWYGEVTDRDPAAYTTENYFPLLK
                     TTATTASGQAKDKFHFIYPTDVWLALSQGGVEAGYGAEFAVLKATAPNRRIVVAFTES
                     GTPAASQFMRGDEILEVDGQDAVNGNTQAVVNALNGGLFPDATGEQHVFLVRGTSGTN
                     RTVTVTSANITHQPVPIVTTFSTGSGPVGYIQFNDHIATAETPFMNAISLLDNANVTD
                     LILDLRYNGGGYLDLASEVAHMVGNTTLTAGQTFEKIVFNSKNPSRNPVTGELLTPTP
                     FHTRTQGFTGAGPANVLLPTLNLNSVYIITGPGTCSASESIINSLRGVGVNVYLIGST
                     TCGKPYGFYPTDNCGTTYFSIQFRGENAANFGDYTDGFSPANTPASAGTLVPGCSVAD
                     DFNHALGDQTEARIAAALSFRASNNQTCPVASGASDPRVSKASATLEEDRSLWVSKPA
                     ARLNRIVRKTAPEPIS"
     gene            complement(5861..6262)
                     /locus_tag="VIV63_02345"
     CDS             complement(5861..6262)
                     /locus_tag="VIV63_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123464.1"
                     /translation="MPEPLMLPARFNGQFGLVLYYQHHGVLLLRSGDRDDGGPRRIDL
                     LFRGVVWMSLPSWFSDFTIEQCLLDEVLEYIPPSHRPKAESRKVFRVDIDRTPHYIIA
                     GSVFASSDDGSYFDPSPLLPEIEVNLRFDAE"
     gene            complement(6273..>6748)
                     /locus_tag="VIV63_02350"
     CDS             complement(6273..>6748)
                     /locus_tag="VIV63_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY6123465.1"
                     /translation="GAAPPVLGWAAVTGTVEPNALLLFLIVFVWTPPHFWALAIARKD
                     EYAKVGIPMLPVTHGVAFTRLQVVLYTVLLTAVTIMPFVTGMSGLIYLAAALVLNAMF
                     IFYALQMKITERTHLPMKVFRFSITYLMWLFAALLVDHYLPTTQAILPAAGISVS"
BASE COUNT         1290 a         2204 c         2130 g         1124 t
ORIGIN      
        1 ccgcgaacaa cgcgaacacc tcatcgcgga gctgcgcaaa cgcgcgcggc ccaccgatgc
       61 cggtgcggcg gatgcattgc agtggacgct cgatcacgag accgcgaaat catgatcacg
      121 cattcgggag gctgccattg cagccgcgtc cgcttcgaag tcgacgcgcc agcggagctc
      181 tccatcgacg actgcaactg ctccatgtgc tcgcgcacgg gatacctgca tctcatcgtg
      241 acgaaggacc gtttccgcat cacgaagggc gcgaacgcgc tgaccacgta caccttcaac
      301 accggcaccg cgaagcatct gttctgcgcc acctgcggca tcaagccgtt ctatgtgccg
      361 cgttcgcatc ccgatggctt cagcgtgaat gcgcggtgca tcgactcgga caccgtcaaa
      421 ggcatgaaga tcaatcccat cgacggccgc aactgggagc agcaatatcc ggcgggccgc
      481 ggtgagttca ccgcttgagg agagatccgt gagccacttc gccgacaaca tccgcccgta
      541 cgtcgatgcc gagctgcgtg ccgccgaaca ggacgcggcc cgcgaatttg cgcacctcga
      601 acgcgcgcac gtgctcgggc aggcatccac gcgcgaacac gtgcgggttc actggcgcat
      661 gttgaagtgg gcatggcggc atcgcgatgc gcgcgagtgg tttggccagg tattccgcat
      721 cctgggcgcg gcgaccaaga ccttcatcgg cctggtgccc gccggcaaca ccggcggcgc
      781 gaacgtgagt ccggtgcgcc cgatgccgct ggatcccgaa ctcgcggcga tcatcgccag
      841 ggcgcgcaag accgatccgc gctagctacc agctgttgcg cagctcgcgc agcttcacga
      901 acaccgtgcg cgcgtccggc ttctcgccga tctccggaaa tttctcgcgc agccgcgcaa
      961 ccagctgcgg attctgcagc cgcagaaagg cgttcacgcg tttctcttca ccgagcgtgg
     1021 tgacgcgcgc gtcctccggc ctctgtgact tcgcgctctc cagcgccttc gccgcatcga
     1081 cgttacccgg ctcgcgatcc agcgtgaacg cgagattgcg cgccatgtac tcatgccccg
     1141 gatatacacg cgtggcgtcg ggcaggcgcg cgagctggtt cacgaacgtc tcgtagagca
     1201 gaccgggatc gccgccgttg tggcagttgc ccgcaccggc attgaacagc gtatcgccac
     1261 agaacagcgc aggtgtttct ccatgcgcca gcagacagac gtgcgaacgc gtatggccgg
     1321 gggtgtcgag cacctcgagc tccacggtgc gcccgacctt gatgacgtca ccacgcgcga
     1381 gtcctctatc cactccgccg atgtggccag ccgcacccgc atgcgcgttc acgcgcgcgc
     1441 gcgtggcggc gacgacgccg gcattgccat cggtgtggtc gcgatgctcg tgcgtgttca
     1501 ggatctgcgt gatgctgaga cccgcggcgc gcgctgccgc gaggcattgc gcggcatcga
     1561 gcgggtcgac gatcagcgcc tcaccggtct cttcgcaggc gatgaggtag tggaaattcc
     1621 ggagagagtt cgcggcccag attcgttcga tgcgcatggg agcgttctac gccctcagcg
     1681 cctcatcgaa cagtccgtgc tgcgacttga gcggcacgcg gatgcgcacc gtggtgcctt
     1741 caccgacgat ggaatgaata tcgatgctgc cgcccatgag cttcaggcgc tcacggatat
     1801 tgaagagtcc aaatccgccc tggcgcgtgg gcaatcggtt gatggaatgc acttcgaagc
     1861 cgacgcccgg gtcgatcacg tcgagcgcca gcgaatcgtc attcaccgag cagcgcaccg
     1921 tggcgcgttt ggagcgcgca tgtttgacca cgttctgcag cagttcgcgc acggtctgga
     1981 acagcagcac cgtcacctgg tcttcgagcg gcttcggatt tccatcgtcc tcgacagtca
     2041 ccatgagccg ctgctgtttc gacacccggt ccgcgagcca gcgcagcgct tcgagcaggc
     2101 cgctttcgtg cagtcccggc ggcgcaagct ccagcatcag cgtccgggtc tgcaacgtgg
     2161 cgtccgcgag cacctgctcc gcttcgtcga ttcgctgcaa ctgcgcgcgg tcgttgaggc
     2221 cgcgtttcat ttcggcgagt tgcatcccga gtcccatgag gttctgcgcc ggcccgtcgt
     2281 ggagctcact cgcgagcttg cggcgcgcgc gttcttcggc gagcgtgatc tcggtggcga
     2341 gcgcacgcaa ttgcgaatcg tgctgggcca gcgcggcttc cgcttcgcgc atcgcggtca
     2401 cgtcgcggcc gctgccccag aagcgcagca ccatcccgtt ctcgacgatt ccgatcatcg
     2461 agatgagcaa cacccgatcg agtccgtagg gtccgtgcac gacatgttcg atgttgcgca
     2521 cctgatagcc gttgcggatc gcctcccgga tgcgttcgat ataaatcttc gaccaggtcg
     2581 ggtgatcgcc caggcgcgta ccgacgatcg ggctctgcgc atccttgacg ccgagcgcca
     2641 ccatgaacgc ggtgttgcac tcggcgacga agccgtgttt gcgcacccag gctacctgct
     2701 cgtccggcgg cagcgtgatc ggcatgggct ctgccagctc ggttcgaaag atggcctccg
     2761 agctgttggc gatgaaggcg cgataacgtt catcgctggc gcgcgcctgt gcgatcgccg
     2821 aatcacgttc cgacatcacc gcctgcagca gaagcaccgg caacacgcac agggcgagga
     2881 atgtctgcag ctggtaggca ctgtcgaatg gcccgtaacc gcgcgtcgtt gccagcgacg
     2941 cgatcgcgac ggtgagccct gcggccagcg cggtgcgccg cgggccactc aacatcgcga
     3001 tccacaccag cactggatag acgatgtagg gaacgtcgag aggtgacatc agcgtgccgg
     3061 ctttgcgcag gaacacgaat gccagcatca gcagcagcac ggcgaatgcc gacaacgtgg
     3121 ccgcgcgtcc gccgcgcgaa tccttcgaga gcgcgaatcc gtggaagccg acagtgagca
     3181 gcagcggcgt cacgatgagc gcgcccagga aatcgccgat ccaccagccc tgcacaccct
     3241 gccaataggc gaggtcgccg aaagattcgt cgccgcgcaa cgctatggcg acgaccgcgc
     3301 agaggagtgt ggacaccagc gccgcgaccg cgaagaacag cacgtggcgc aggcgcgcga
     3361 acgagatggg cccgcgcacg gcggcgtgca tgaccccgac gcccaccagc gccgcggaga
     3421 acttcaccgg gaacaacagc cccgccgcgg agaacggcag ttgcgctgcg aacaccgtgt
     3481 gggcgaggag ctcggccgcg taacaggtga cgaacagggc cggccagaaa cgggcggggt
     3541 acagcaacag gaaggcgaac aggctgccgg ctgccggcca gaaaaggccc acgccctgcg
     3601 agccggtgag aagggtcagc ccgatcccca cggcaaagaa atgcaacgga aagtagatca
     3661 ctcccgcctg gaggggacct aacttcagtc cgaatggtcg aagtagcctg ttcatggctg
     3721 gcgccatcat agaacgagac atggagcgta gccccatggc gactctttgt tgtttgctcg
     3781 gctccgtgca tattgcgagt catgtctcag tccagaatcc gcgacgaggg ggtcgcccgg
     3841 tgaaaagccg ctataaattc agagtcttgc tgtttccctt gctcggaact ctggccgcgt
     3901 gcggcggcgg tggcgggggt ggcagttcca gcagcagctc gagcagcagc agcggtggct
     3961 ccacctggca acccggggtt tacctgcctt cgagcaactt cgatgccatg tgcgcggcgc
     4021 ctcgcgctgg cacggccgac cggcgcggta gcgttaccga ccaaaacaac tggctgcgct
     4081 catggaccaa tgagctttac ctctggtacg gcgaagtcac ggatcgcgat ccggcggcat
     4141 acaccaccga aaactatttc ccgctgctga agaccacggc caccaccgcc tcgggccagg
     4201 ccaaggacaa attccatttc atctacccca ccgacgtgtg gctggcgctg tcgcagggtg
     4261 gcgtggaagc aggctacggc gccgaattcg cggtgctcaa ggcaacagcg ccgaatcgcc
     4321 gcatcgtggt ggcgttcact gaatcgggga cgcccgctgc cagccagttc atgcgaggcg
     4381 atgaaatcct ggaagtcgac ggacaggatg ccgtcaatgg caatacgcag gcggtcgtca
     4441 acgcgctcaa tgggggactg tttcccgatg cgaccggcga gcaacacgtc ttcctggtgc
     4501 gcggaaccag cggcaccaac cgcacggtga ccgtgacctc ggcgaacatc acgcaccagc
     4561 cggtgccgat cgtcaccacg ttcagcacgg ggtcgggccc ggtcggctac atccagttca
     4621 acgatcacat cgcgacggcg gagacgccgt tcatgaacgc aatctcgttg ctcgacaacg
     4681 ccaacgtcac cgatctcatt ctcgacctgc gctacaacgg tggcggctat ctcgatcttg
     4741 ccagcgaggt cgcacacatg gtcggcaata ccacgctgac cgcgggtcaa accttcgaga
     4801 agatcgtgtt caacagcaag aacccgagcc gcaatccggt cacgggcgaa ctgctgacgc
     4861 ccacaccgtt ccatacgcgg acgcagggct tcacgggcgc cggtcccgcg aacgtgctgc
     4921 tgcccacgct caatctgaac agcgtgtaca tcatcaccgg acccggcacc tgttcggcga
     4981 gcgagtccat catcaacagc ctgcgcggcg tcggcgtgaa tgtgtatctc atcggctcca
     5041 cgacctgcgg caagccgtat ggcttctatc cgaccgacaa ctgcggcact acctacttct
     5101 ccatccagtt ccgcggcgag aacgccgcga acttcggtga ctacacggac ggcttctcgc
     5161 cggccaacac gccggccagt gcgggcaccc tcgtgccggg ctgctcggta gcggatgact
     5221 tcaatcacgc gctcggcgat cagaccgaag cgcgcattgc cgccgcgctg agtttccgcg
     5281 ccagcaacaa ccagacctgc ccggtcgcga gcggcgcgtc ggatccaagg gtgtccaagg
     5341 cgtcggccac gttggaagaa gacaggagcc tgtgggtgtc gaagcccgcg gcgcgcctca
     5401 accgcatcgt ccgcaagacc gcgccagaac ccatctccta gtcagcgctc gcaggtagtt
     5461 agcgtgccgt gaagaacacg ggaacggacc tcaccgcgtg aggccgcccc cgtgtccgcc
     5521 gtgaggagga agcgtggtcc tcagccattt ccgcagccgg cacacggcgt gattccagcc
     5581 ggaatgcaat gactctcgct tggaacgagc aaagccggtc tggaagggac ttgtgtgggg
     5641 atccgctttc ggttcggaca gcaactctgt gagatcgtgc gtcaccaggc ccattgctgt
     5701 gccctccttg tgcggcttcc cgatcggggt gtctcgggtc cgatcatcac tccattcgag
     5761 tcaggagtca tcagaccatt gggccattgc gcgacctatc tacgccgcag tgcgtcaacc
     5821 aggattggta cgctgcgctg caaaacagcg cgtgggttct ctactccgca tcgaacctga
     5881 gattaacctc gatctccggc agcagcggtg acggatcgaa ataggaaccg tcatcgctgg
     5941 aggcgaacac gctgccggcg atgatgtagt gcggcgtgcg gtcgatgtcg acgcggaata
     6001 ccttgcgcga ttccgccttg gggcggtgac tcggcggaat gtattcgagc acttcgtcca
     6061 gcaggcactg ctcgatggtg aaatcgctga accacgaagg cagcgacatc cacaccacgc
     6121 cgcggaacag gaggtcgatg cgcctcgggc cgccatcgtc gcggtcaccc gaccgcagca
     6181 gcagtacgcc gtgatgctgg tagtacagca ccaggccgaa ctggccgttg aaccgtgcag
     6241 gaagcatcag cggttcgggc actgaaaggc tctcaggaga cactgatacc cgccgcgggc
     6301 aagatcgcct gcgtggtggg tagatagtga tcgacgagca gcgccgcgaa cagccacatg
     6361 aggtaggtga tcgagaagcg gaacaccttc atcggcaggt gcgtgcgctc ggtgatcttc
     6421 atctgcagcg cgtagaagat gaacatggcg ttgagcacca gtgccgccgc caggtagatc
     6481 agcccgctca tgccggtgac gaaaggcatg atggtgacgg ccgtcagcag cacggtgtaa
     6541 agcaccacct gcagccgcgt gaacgcgacg ccgtgtgtga ctggcagcat cgggatgccc
     6601 accttggcgt attcatcctt gcgcgcgatg gccagcgccc agaaatgcgg cggcgtccac
     6661 acgaacacga tgaggaacaa cagcagcgcg ttgggttcca cggtgcccgt gaccgccgcc
     6721 cagccgagca cggggggggc ggcgccgg
//
LOCUS       DATDEU010000100         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_894122, whole genome shotgun sequence.
ACCESSION   DATDEU010000100 DATDEU010000000
VERSION     DATDEU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894122"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VIV63_02355"
     CDS             complement(<1..252)
                     /locus_tag="VIV63_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of succinyldiaminopimelate
                     from N-succinyl-2-amino-6-ketopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY6123466.1"
                     /translation="MNGRLELLAAYPFERLARLKSGITPPAGLPHIAMSIGEPKHAPP
                     PFVIEALRRELSKLDSYPVTAGLPETRASFAAWLARRFRV"
     gene            complement(249..2948)
                     /gene="glnD"
                     /locus_tag="VIV63_02360"
     CDS             complement(249..2948)
                     /gene="glnD"
                     /locus_tag="VIV63_02360"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY6123467.1"
                     /translation="MASLDEDIEATETTVLRSSPWLRLANLPERLAAAKFAPDAFRKE
                     LQEAHAELAERFRADESVEVLVHARAELIDAILHDVWRSQLPAGYESWTLAAVGGYGR
                     GELHPHSDVDILILVPEAPDEAGRSVVERVVTFLWDINLEVGHSVRTVAECAEESIAD
                     VGVMTTLIEARLLAGNAQLLATMREAVSADRVWSVKAYFEAKVAEQQDRHLKANDTAY
                     NLEPNVKTGPGGLRDIQTIAWVAKRHFGAASLDELLTHGFLSASELRKLKQAQSFLWK
                     VRFGLHVVTGRREDRLLFDHQIKLAQSFGYEDASYTLAVEQFMQRYYRTVMDVMLLNE
                     LLLQLFREAILTETAPPRPLSPRFQVRNEYLEAVNEELFARTPSTLLELFVVLQQNPD
                     VKGVRASTIRSVNKNLWLIDEEFRQNPRNHRLFLEILRAPAGVTHELRRMNTYGVLGR
                     YIPAFGRIVGRMQYDLFHAYTVDAHTLFVLSNLRRLSLPKYDHELPQLSAIMQQLPKL
                     EIAYLAALFHDIAKGRGGDHSQLGAVDAEAFCLEQGLSRYDARLVAWLVQNHLELSVT
                     AQKQDISDPEVINAFARRVGDESHLDYLYVLTSADVRGTNPKLWNSWKASLFQEFYER
                     VKRALRRGLESPIDPDELMRETQEGARKLLAASGRITAPEITRAWERLTAAYFLRHTP
                     EEVAWHTQLLADREPGSEDPLVAVQSSSERGTTAILAFAPHRYNSFAITTAVLDQLGL
                     NIVDARITPTGDGFSLDLYHVLEEDGAPISDSERIGEIERALWRALQHPDEAPVTVTR
                     RVPRQARMFRTSTQIAVTVDERNRRSVLELIAGDRPGLLCDVGKVLMEERVDLLAAKI
                     MTVGERAEDVFYLASLSGAPLDEAAATRLQQRLSGTLDRRAAA"
BASE COUNT          499 a          924 c         1037 g          556 t
ORIGIN      
        1 tacccggaag cgacgggcga gccaggcggc gaaactggcg cgcgtctcgg gcagtccggc
       61 ggtcaccgga taactgtcga gtttcgagag ctcgcggcgc aacgcctcga tgacgaacgg
      121 cggcggcgcg tgtttgggct cgccgatgga catggcgatg tgcggtagtc ccgccggcgg
      181 cgtgatgccg ctcttgagcc gcgccaggcg ctcgaaagga taggcggcga gtagttcgag
      241 gcggccgttc acgcggcggc gcggcgatcg agcgtgccgg acaggcgctg ctgcagccgg
      301 gtggccgcgg cttcgtcaag cggtgctccc gacagcgagg cgaggtagaa cacgtcttcg
      361 gcgcgctcgc ccacggtcat gatcttcgcg gccagcaggt cgacgcgttc ttccatgagc
      421 accttgccca cgtcgcacag gagtccgggc cggtctccgg caatcagttc cagcaccgat
      481 cggcgattac gttcgtcgac ggtgaccgcg atctgcgtcg aggtgcggaa catgcgcgcc
      541 tgccggggca cgcgtcgcgt gaccgtgacg ggcgcttcgt cggggtgctg cagcgcgcgc
      601 cacaacgcgc gctcgatttc accgatgcgt tcgctgtccg agatcggcgc gccgtcttct
      661 tcgagcacgt gatacagatc gaggctgaag ccatcgccgg tcggcgtgat ccgggcgtcg
      721 acgatgttca ggccgagctg atctagcacc gcggtggtga tcgcgaagct gttgtagcga
      781 tgcggcgcga acgcgaggat ggcggtggta ccgcgctcgc tgctcgactg caccgcgacc
      841 agtgggtctt cgctgcccgg ttcgcggtcg gccagcaact gtgtatgcca ggccacttcc
      901 tcgggcgtat gccgcaggaa gtacgcggcg gtgagccgct cccatgcgcg cgtgatttcc
      961 ggcgcggtga tgcggccgct cgcggccaga agcttgcgcg cgccttcctg ggtttcgcgc
     1021 atgagctcgt cgggatcgat ggggctttcc aggcctcggc gcaacgcgcg tttcacgcgc
     1081 tcatagaact cctggaacag cgacgccttc caggaattcc acagcttcgg gttggtgccg
     1141 cgcacgtcgg cgctggtcag tacatataag tagtcgagat gcgactcgtc gccgaccctg
     1201 cgcgcgaagg cattgatgac ttccggatcg gagatgtcct gcttctgcgc ggtgaccgaa
     1261 agctcgaggt ggttctgcac cagccacgcc accaggcgcg cgtcgtaacg tgacagcccc
     1321 tgctcgaggc agaacgcctc ggcgtccacg gcgccgagtt gcgagtgatc gccgccgcga
     1381 cccttggcga tgtcatggaa aagtgcggcg aggtaggcga tctcgagttt gggcagctgc
     1441 tgcatgatgg ccgagagctg cggcagttcg tggtcgtatt tcggcagcga cagacgccgc
     1501 agattgctca acacgaacag cgtgtgcgca tcgacggtgt acgcatggaa caggtcgtac
     1561 tgcatgcggc cgacgattcg accgaacgcg ggaatgtagc ggccgagcac tccgtaggta
     1621 ttcatgcggc gcagctcgtg ggtcaccccc gccggtgcgc gcaggatttc caggaacagg
     1681 cgatggttgc gcgggttctg gcgaaactct tcgtcgatga gccacagatt cttgttcacc
     1741 gaacggatgg tcgaagcgcg cacgcctttt acatccgggt tctgctgcag caccacgaag
     1801 agctccagca acgtggacgg cgttcgggcg aacagttctt cgttaacggc ttcgaggtat
     1861 tcgttgcgca cctggaagcg cggcgagagc ggtcgcggcg gcgccgtctc ggtgaggatg
     1921 gcttcgcgga acaactgcag cagcagttcg ttgagcagca tgacgtccat gacggtgcgg
     1981 tagtaccgct gcatgaactg ttcgaccgcc agcgtataag aagcgtcttc gtagccgaag
     2041 ctctgcgcga gtttgatctg gtgatcgaac agcaggcggt cttcacgccg gccggtgacc
     2101 acgtgcaatc cgaaacgcac cttccacagg aacgattgcg cctgtttgag cttgcgaagc
     2161 tccgacgcgg acaggaaacc gtgggtcagc aattcgtcga gcgaagccgc gccgaaatga
     2221 cgcttggcaa cccaggcaat ggtctggatg tcgcgcaggc caccaggacc cgtcttcacg
     2281 ttgggctcga ggttgtacgc ggtgtcgttg gccttgagat gacggtcctg ctgctcggcg
     2341 accttggctt cgaaataggc tttcaccgac cacacgcggt cggccgacac ggcttcgcgc
     2401 atcgtggcta acaactgtgc gttgccggct agcagccgcg cctcgatgag cgtggtcatg
     2461 acgccgacgt cggcgatgct ctcttcggcg cactcggcga ccgtgcgcac gctgtggccg
     2521 acctcgagat tgatgtccca caggaaagtg acgacgcgtt cgacgaccga tcgcccggct
     2581 tcgtcgggcg cttcgggcac gaggatcagg atgtcgacgt cggaatgcgg atgcagttcg
     2641 ccgcggccgt agccaccgac tgccgcgagc gtccagcttt cgtagccggc tggcaattgc
     2701 gaacgccaca cgtcgtgcag gatggcgtcg atgagttcgg cgcgcgcgtg caccagcact
     2761 tcgacggact cgtcggcgcg gaagcgttcg gcgagttccg cgtgcgcttc ctgcagttcc
     2821 ttgcggaatg cgtcgggcgc gaacttcgcg gccgcgagac gttcgggcag attggcgagc
     2881 cgcagccagg gcgagctgcg caataccgtg gtttcggtgg cttcgatgtc ctcgtcgagc
     2941 gaggccatct agttgtagaa cgcagcgcgc ttgctgccct cgccgagggt cagcacttcc
     3001 acgccggtct cggtga
//
LOCUS       DATDEU010000101         8062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1915362, whole genome shotgun sequence.
ACCESSION   DATDEU010000101 DATDEU010000000
VERSION     DATDEU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915362"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VIV63_02365"
     CDS             complement(<1..582)
                     /locus_tag="VIV63_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6123468.1"
                     /translation="MNSSNVDILLIEDDARLAELTATYLEQNGLRVAVEERGDRALER
                     FAREKPRLVLLDLLLPGKDGLTICRELRRVHEVPILILTARDTDIDHVIGLEAGADDY
                     VMKPVDPMVLLARVRALMRRAERGAPNGGERKPDLTLGALRISETSREVWLKGQSVAL
                     TTQEFELLSLLAHRAGELVSRDEVFRTMRGIDYD"
     gene            complement(579..980)
                     /locus_tag="VIV63_02370"
     CDS             complement(579..980)
                     /locus_tag="VIV63_02370"
                     /inference="COORDINATES: protein motif:HMM:NF022895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3019 domain-containing protein"
                     /protein_id="HEY6123469.1"
                     /translation="MRASLKLLAIGLFGVAFPVTAADPPVRLFVKPLLCVVDKAATSC
                     KVVFDIRWKSARPAEYCLNDEAVATPLRCWPSAVSGELQHEREVTEDFVFWLAPPAAT
                     ERAAEVKVEVLRVGSDDRRRERRTRHVWDVL"
     gene            complement(980..1915)
                     /locus_tag="VIV63_02375"
     CDS             complement(980..1915)
                     /locus_tag="VIV63_02375"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY6123470.1"
                     /translation="MKFPTLQQLLATAALWLLAQAAHAQTEAEVPAPAATASHTRPLP
                     AERSWRLGIGVGYGNRTNPLVLSDDIPVIVDLDIAWFGKRWFFDNFDLGFQLADNRWF
                     TANAVTRVNSDRVFFGKTNVKYVNFTRLAGGAQVAFAPPPSPPLPANPAGGPSVAEAV
                     PQELKVPDRDYAIEMGLEMLMDGEWGQATLRGFHDVSQTHKGFEISADYSYRWTHGRF
                     SLSPSVGLAYKSDKLSDYYWGVNPKEAGQTLVPYEASGGVDWNVGLRTSYYLTKSVRL
                     AISGDYEKLHDSVALSPIVEDDHVIGYFAGVAWQF"
     gene            2100..3998
                     /locus_tag="VIV63_02380"
     CDS             2100..3998
                     /locus_tag="VIV63_02380"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEY6123471.1"
                     /translation="MRRPLNWLVFAIALLALTANLMLTGCGGGGDADDGGNDPPPPAS
                     GMLRKVSSAGELETSLKASLRLAVDTGGSALIVPAGGASGGDYSNTYTSEAGVDEFDY
                     ARYDGAYLYIAPTPYANNQVPRSIRILRTDPATGGATQVSSIPIESELAVQGLYVADG
                     RLVMLTSGANYHSFGGIWATLVAWAPTRLAIQVYDVSDPANPARLLNADMDGAFVASR
                     RVGDRVYLVSRHSPSVLLDPQARLRIADLSLAELLPKVTVGGRTRPLVSSSDCYVTNE
                     ENHKGYPILTTITSFSMQNPADFVNTCYNEQANGVYASTSALYISQPVLGPIPGAAAT
                     RIHKFALTGAAPAYAGSVQVSGLLWMGGQRDFRMSEHRGMLRVMTTEQTNDLDDWQDH
                     RLYVLRPKSAELALEVVSSLPNDSRPEEIGKSGEGLFGVRFVGDRAYAVTFRAVDPLY
                     VLDLANPADPRIAGTLELPGFSDFLHPVTEDLLLGLGGTMSNAKLELFDVSEIEQPQS
                     RGSITIGGWSSSSEALHDRHAFTSLPADAADRFAIPATVVPGTTNGLNSNTESSLYQF
                     EILGKQSAASASLQAAGAVTPPVANDAERYVMSSRSFIHGDTVYYVRDGKVWSSSWFT
                     PSQVQGPF"
     gene            complement(4005..5633)
                     /locus_tag="VIV63_02385"
     CDS             complement(4005..5633)
                     /locus_tag="VIV63_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY6123472.1"
                     /translation="MQAQVTFGSFRFEPATARLWEHDSEVKLTRKAALVLGALLERPG
                     APVSKQELFASVWRGTVVSDDALVTCIQELRKALGDDSKQPLYIETRHRLGYRFAAAV
                     ESPVVEVPVPASRGGATIAVLPFADMSPARDQDYLCEGLAEELIDALTHVEGLRVAAR
                     TSSFQFRGDRDLREVGARLNVGSLLEGSVRKAGDRLRVTVQLIDVATGFHQWSEKFER
                     DAGDVFAMQDEIAEKVATLLRGGELSTRERRAVRRQPTAIETYECFLRGRQRMHTMQQ
                     PHMDEAHALFTRAIALDAEYAPAWAGLATLHALLYEWWGSRDEDIAEAERASRIAMEL
                     APDLADAHLSRGYTLSNQRRYREAQTHFEAAARINPNLFDAYYYYGRAAFAAGDTEKS
                     IELWRKAGEVRREDFESPLLQGQSLRKLGRMDEAREVNREAMRRAERLLEVNPQNGRV
                     LSFGSGALDLEGQLDRALEWARRAEQLYPNDMGVIINGACLRARNGMKDEALDLLERV
                     FNKGWGKKDWIENDPDYDSLRDDPRFIAMMAKLK"
     gene            5772..6536
                     /locus_tag="VIV63_02390"
     CDS             5772..6536
                     /locus_tag="VIV63_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123473.1"
                     /translation="MKRLAIFAYGLVSYAVFFATFLYGIGFIGNLWVPKSIDSPRDVA
                     LGTALLVNLGLLGLFAVQHSVMARPAFKRWWTRIVPESAERSTYVLFSSLALIALFWF
                     WQPMGGVVWQITAPLGVDLMYMLYAAGWLLLLYTTFLINHFDLFGLRQVWLQLVGKPF
                     TPLDFKTPWLYRQVRHPLYVGWLMIFWATPTMTIAHLVFAVMTTAYILIAIQFEEHDL
                     IDAHPEYAEYRKRVPMLLPFGKRGAADVNPAARRPV"
     gene            6625..6753
                     /locus_tag="VIV63_02395"
     CDS             6625..6753
                     /locus_tag="VIV63_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123474.1"
                     /translation="MYPERFDARRFDGLAKLVASVERKNLERQLARREWSLEMRAA"
     gene            6760..7572
                     /locus_tag="VIV63_02400"
     CDS             6760..7572
                     /locus_tag="VIV63_02400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6123475.1"
                     /translation="MASRPVDFFDGAYANFTEQVLASIREATFGEDIGQNSWVTADEY
                     AGYIESLRLTARSHVLEVASGSGGPALHLARKTGCQVTGVDANPSGIATANRAATHAG
                     LADRLKFRVADATRPLPFEDNTFDALLCIDSMNHFPNRSDSLKEWCRILKPGGRAIFT
                     DPVVLTGPVTNEELAARSSIGLFVFVPKGLNEELIVKAGFKLVSQLDATENAALVSRR
                     WREARQRFRDDLLRMEGAENFEGVQKFLDAVHRLTTERRLSRIAYRVEKPAG"
     gene            7693..>8062
                     /locus_tag="VIV63_02405"
     CDS             7693..>8062
                     /locus_tag="VIV63_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123476.1"
                     /translation="MNKIQVDMYAVGLGAAILVQYRLEDGKVVRVLADGGMGHGGYPV
                     DGVHDRIADAFTSFGDDESRIDLIIGTHYDGDHLKGLVPIAEDDSLQIGEVWLPPIRD
                     DSDEVPGHISEDSGFLAHKFF"
BASE COUNT         1398 a         2613 c         2548 g         1503 t
ORIGIN      
        1 gtcgtagtcg atgccgcgca tggtgcggaa cacttcgtcg cggctcacca gttcgccggc
       61 gcgatgcgcc agcagactga gtagttcgaa ttcctgggtg gtgagcgcca cggactggcc
      121 cttcagccac acctcgcgcg atgtttcgct gatgcgcagc gcgccgagcg tgagatccgg
      181 cttgcgttcg ccaccgttcg gggcgccacg ctccgcgcgg cgcatgagcg cgcgcactcg
      241 cgccagcaac accatgggat ctacgggctt catgacgtag tcgtcggcgc cggcttcgag
      301 accgatgacg tgatcgatgt cggtatcgcg cgcggtgagg atgaggatgg gcacttcgtg
      361 cacgcggcgc aattcgcggc agatggtgag tccgtccttg ccgggcagca gcagatcgag
      421 caacacgagc cgcggctttt cgcgcgcgaa tctttcgagc gcgcggtcgc cgcgttcttc
      481 caccgcgacg cgcagtccgt tctgctccag gtacgtggca gtcagttccg cgagccgcgc
      541 atcgtcttcg atcagcagga tgtcgacgtt cgatgaattc ataggacgtc ccacacatgc
      601 cgggtgcggc gctcgcgcct ccgatcatcg gagcccacgc ggagaacctc gactttcact
      661 tcggcggcac gttcggtggc ggccggcggc gcgagccaga aaacgaaatc ttcggtgacc
      721 tcgcgctcgt gctgcaactc gcccgacacc gcgctgggcc agcagcgaag cggcgtcgcc
      781 acggcttcgt cgttgagaca gtactcggcc ggacgcgcgc tcttccagcg aatgtcgaac
      841 acgaccttgc acgaagtcgc cgccttgtcg acgacacaca gcaacggctt cacgaacagc
      901 cgcacgggcg gatcggcggc cgtgacggga aacgccacgc caaacaaacc gattgccagc
      961 aatttgagcg aagcgcgcat cagaactgcc acgccactcc ggcgaaatag ccgatgacgt
     1021 gatcgtcttc gacgatgggg ctcagggcca cgctgtcatg caacttctcg tagtcccccg
     1081 agatcgccag ccgcacgctc ttcgtgaggt agtagctggt gcgcagtccg acgttccagt
     1141 cgacgccgcc actcgcttcg tagggaacca aggtctgtcc cgcctctttc ggattcacgc
     1201 cccagtagta gtcgctgagc ttgtcgctct tgtaggcgag gccgaccgac ggcgacaggg
     1261 agaagcggcc gtgcgtccat cgataactgt agtcggcgga aatttcgaaa cccttgtgcg
     1321 tctggctgac gtcgtggaac ccacgcagtg tggcctggcc ccactcgcca tccatgagca
     1381 tctcgagtcc catctcgatc gcgtagtcac ggtcgggaac cttgagttcc tgcgggacgg
     1441 cctcggcaac gctcggccca ccggccggat tggcgggcag cgggggcgag ggaggcggcg
     1501 cgaacgcgac ctgcgcgccg ccggccaggc gcgtgaagtt cacgtacttg acgttcgtct
     1561 tgccgaagaa cacgcgatca ctgttcacgc gcgtcacggc attggccgtg aaccagcggt
     1621 tgtccgcgag ctggaagcct aagtcgaaat tgtcgaagaa ccaccgcttg ccgaaccacg
     1681 cgatgtcgag gtcgacgatg accggaatgt catccgacaa cacgagcggg ttggtgcggt
     1741 ttccgtaacc gacgccaatt cccaaacgcc agctgcgctc ggcgggcaac gggcgcgtgt
     1801 ggctcgccgt ggccgcgggc gcaggtactt cggcttcggt ctgcgcatgc gcggcctggg
     1861 ccaaaagcca gagagccgct gtcgccagca gttgctgaag agtcggaaat ttcatgtcgc
     1921 cattctaact tcggccggtg gaaaggcccg gcaaagcttc gcgcgaagct gtagggaaaa
     1981 tgtgaaaaag tgtaaggccc tcattcgaca caatttgacc aaacccctca agtctcgcga
     2041 cacatgcccc ggaggcccgg cagtagcctg ctagccgtgg atttccgggg agaaacaaca
     2101 tgcgccgccc attgaactgg cttgttttcg cgattgccct gctggcgctg accgccaacc
     2161 tgatgctgac cgggtgcggc ggtggcggcg acgccgacga tggtggcaac gatccccctc
     2221 cgcctgcgtc gggaatgctt cgcaaggtca gctcggccgg cgagctcgaa acctcgctca
     2281 aggcctcgtt gcggctcgca gtcgacaccg gcggctccgc cctgatcgtg cccgcgggcg
     2341 gcgccagcgg cggcgattat tcgaatacct acaccagcga agccggcgtc gacgaattcg
     2401 actacgcgcg ttacgacggc gcctatctct acatcgcgcc gacaccgtat gccaacaacc
     2461 aggtgccgcg ctcgatccgg atcctccgca ccgatccggc cactggtggc gcgacgcagg
     2521 tgagctccat acccatcgag agcgagcttg cggtgcaggg cctctatgtg gccgacggcc
     2581 gcctcgtgat gctcaccagc ggggccaact accactcgtt cggcggcatc tgggccacgc
     2641 tcgttgcctg ggcgcccacc aggctcgcga tccaggtcta tgacgtcagc gatcccgcga
     2701 accccgcgag gttgctgaat gccgatatgg atggtgcgtt cgtcgccagc cgccgggtcg
     2761 gcgaccgggt ctatctagtg agccggcaca gtccttcggt gctgctcgat ccccaggcgc
     2821 gcctgcgtat cgccgacctg tcgttggctg agctcctgcc caaggttacg gttggcggcc
     2881 gcacgcgtcc cctggtgtcg tcgtcggact gctacgtcac gaacgaggag aatcacaagg
     2941 gttatcccat cctgacgacg atcacttcgt tctcgatgca gaacccggcc gacttcgtga
     3001 acacctgcta caacgaacaa gccaacggtg tttatgcctc gacgtcggcg ctctacattt
     3061 cgcagcccgt tctcgggccc attccgggcg ccgcggccac gcgcatccac aagttcgcgc
     3121 tgacgggcgc ggctcccgcc tacgccgggt ccgtgcaggt ctccgggctg ctctggatgg
     3181 gagggcagcg tgacttccgc atgagcgaac accggggaat gctgcgcgtg atgaccaccg
     3241 agcagacgaa tgatctggac gactggcagg atcaccggct gtacgtcctg cgtcccaagt
     3301 ccgccgagct cgcgctcgag gtggtctcca gcctgcccaa cgattctcgc cccgaggaaa
     3361 tcggcaagtc cggcgaaggg ttgttcggcg tgcgcttcgt gggagatcgc gcctacgcgg
     3421 tcacgttccg cgccgtcgat ccgttgtatg tgctcgacct cgcgaacccc gccgacccgc
     3481 gcatcgccgg aacgctcgag ctcccgggct tctccgattt cctgcacccc gtgacggaag
     3541 atttgttgtt agggctgggc ggcacgatga gcaacgccaa gcttgagctg ttcgacgttt
     3601 cggagatcga gcagccgcaa tcgcgcggca gcatcacgat cggtggctgg agctccagtt
     3661 ccgaggctct ccacgaccga catgcgttca cctctcttcc ggcggacgcg gccgatcgct
     3721 tcgccattcc ggccaccgtc gttcccggca cgacgaacgg cctgaactcc aacacggaat
     3781 cgtcgctgta ccagttcgaa atcctcggca aacaaagcgc cgcgagtgcg tcgctgcagg
     3841 ctgcgggagc ggtcacgccg ccggtggcga acgatgccga gcgctacgtg atgtcgtcgc
     3901 gttcattcat tcatggcgac acggtttact acgtgcgcga cgggaaggtc tggtcgagct
     3961 cctggttcac accgtcgcag gtgcaggggc cgttctgaac gacgctattt cagcttcgcc
     4021 atcatggcga tgaagcgcgg atcgtcacgc aggctgtcgt agtccggatc gttttcgatc
     4081 cagtccttct tgccccagcc cttgttgaac acgcgttcga gcaggtcgag cgcttcgtcc
     4141 ttcatgccgt tgcgcgcgcg caggcaggcc ccgttgatga tgacgcccat gtcgttgggg
     4201 tagagctgct cggcgcggcg cgcccactcg agcgcgcggt cgagttgccc ttcgagatcg
     4261 agcgcgccgg aaccgaatga caatacgcgg ccgttctgtg gattgacctc gagcagacgc
     4321 tcggcgcggc gcatcgcttc gcgattgacc tcgcgtgctt cgtccatgcg gccgagcttg
     4381 cgcagcgact gaccctgcag cagcgggctt tcgaaatctt cgcgccgcac ttcgccggcc
     4441 ttgcgccaca gctcgatcga tttctcggtg tcgccggccg cgaaggcggc gcggccgtaa
     4501 tagtaatagg cgtcgaacag gttgggattg atgcgcgcag ccgcttcgaa atgcgtctgc
     4561 gcctcgcggt atcgccgctg gttcgagagc gtatagccgc gtgacaaatg cgcgtcggcg
     4621 agatcgggcg cgagctccat cgcgatgcgg ctggcgcgct cggcttcggc gatgtcttcg
     4681 tcgcgcgaac cccaccactc ataaagaaga gcgtgcaggg tggcgaggcc cgcccaggcc
     4741 ggcgcgtatt cggcatcgag cgcgatggcg cgcgtgaaga gcgcgtgggc ttcatccatg
     4801 tgcggctgct gcatggtgtg catgcgttgc cggccgcgca ggaaacactc gtaggtctcg
     4861 atggcggtgg gctggcggcg caccgcgcgc cgttcgcgag tcgacagttc tccgccgcgc
     4921 agcagcgtcg cgaccttctc ggcgatttca tcctgcatcg cgaacacgtc gcccgcatcg
     4981 cgctcgaatt tctcggacca ctggtgaaag ccggtcgcca catcgatgag ctggactgtc
     5041 acgcgcaggc gatcgcccgc cttgcgcacg ctgccttcga gcaaactacc gacgttgagc
     5101 ctggcgccca cctcgcgcag atcgcggtcg ccgcgaaact ggaacgatga cgtgcgcgcg
     5161 gcgactcgca ggccttcgac gtgcgtgagc gcgtcgatga gttcctccgc gagaccttca
     5221 cacaggtagt cctggtcgcg cgcggggctc atgtcggcga acggcagcac ggcaatggtc
     5281 gcgccaccgc gcgacgctgg aaccggaact tccaccacgg gtgattcgac ggccgcggcg
     5341 aagcgataac cgagccgatg gcgtgtttcg atgtacaggg gttgtttcga atcgtcgccc
     5401 agcgccttgc gcaattcctg gatgcaggtg accagcgcgt cgtcgctgac caccgtgccg
     5461 cgccagacgc tggcgaacag ttcctgtttg gacaccggtg cacccggacg ctcgagcagg
     5521 gcgccgagca ccagcgccgc ctttcgcgtg agcttcacct cggaatcgtg ttcccacagg
     5581 cgcgccgtgg ccggttcgaa gcgaaagctt ccgaatgtga cttgagcttg catgcgatcc
     5641 cggaaatatc ccggcgttct cccgggattc gggagggccg gcgccgataa gcttttgtca
     5701 catcgaaacc gttgaaacaa gcgtcctccc ggggcgcccg gtgccgtctg tgacattgga
     5761 ggactcccca catgaaacgt ctcgccatct ttgcgtacgg actcgtctcc tacgccgtgt
     5821 ttttcgccac ctttctctat ggcatcggct tcatcggcaa cctgtgggtg cccaagtcga
     5881 tcgattcgcc ccgagacgtc gccctgggca ccgcgttgct ggtgaacctc ggtttgctgg
     5941 gtcttttcgc cgtgcagcac agcgtcatgg cgcggccggc attcaaacgc tggtggacgc
     6001 gcatcgtgcc ggagtccgcc gaacgcagca cctacgtcct gttttcgtca ctcgctttga
     6061 tcgcgctgtt ctggttctgg cagcccatgg gcggcgtggt ctggcagatc accgcgcctc
     6121 tgggtgtgga cctcatgtac atgttgtacg ccgccggctg gttgctgctg ctgtacacca
     6181 cgttcctcat caaccacttc gatctgttcg gtcttcgcca ggtctggctg cagctcgtgg
     6241 gcaagccgtt cacgccgctc gacttcaaga cgccgtggct gtatcgccag gtgcgtcatc
     6301 cgctgtacgt gggatggctc atgattttct gggcgacgcc caccatgacc atcgcccacc
     6361 tggtgttcgc ggtgatgacc acggcgtaca tcctgatcgc catccagttc gaggagcacg
     6421 acctcatcga cgcgcatccc gagtacgccg aataccgcaa gcgcgtgccg atgctgctgc
     6481 ctttcggcaa acgaggcgcc gcggatgtaa acccggcggc gcgccgtccc gtctgactac
     6541 cggaccccaa ttggatcgag acaaatgaat atggatgcag atctggtggc gcgccgttgg
     6601 cgctccgcgc gaaaaggaac cgggatgtat cccgagcgct tcgatgcgcg ccgttttgac
     6661 gggctggcca aactggtggc cagcgtcgag cgcaagaatc tcgagcggca actcgcacgg
     6721 cgcgagtggt cgctcgagat gcgcgcggcc tagcgtgtcg tggcgtcgcg accggtcgat
     6781 ttctttgacg gcgcctatgc gaatttcacc gaacaggtgc tggcgtcgat tcgcgaagcc
     6841 acgttcggcg aggatatcgg ccagaacagc tgggtcacgg ccgatgagta cgccggctac
     6901 atcgaatcct tgcgcctgac ggcccggtct cacgtgctcg aggttgccag cggatccggc
     6961 gggcccgcgc tgcacctggc ccgcaaaacc ggttgccagg tgacgggcgt ggacgccaac
     7021 ccgagcggta tcgccaccgc caatcgcgct gcaacccatg ccgggctcgc ggaccgtttg
     7081 aaattcaggg tggcggacgc gacccggccg ctgccattcg aagacaacac cttcgatgcg
     7141 ctgttatgca tcgactcgat gaaccatttc ccgaatcgga gcgactcact gaaggaatgg
     7201 tgccggatcc tgaaaccggg cggccgggcg atcttcaccg acccggtagt gctcaccggt
     7261 ccggtcacca acgaggagct cgcggctcgc agctccatcg ggcttttcgt attcgtgccg
     7321 aaaggactca acgaggaatt gatcgtgaaa gcgggattca agctggtgag ccagctcgat
     7381 gcgacggaaa acgccgccct ggtctcacgc cgctggcgtg aagcgcggca gcgcttccgc
     7441 gacgacctgc tgcgcatgga aggcgcggag aacttcgaag gcgtgcagaa atttctggat
     7501 gcagtacacc ggctgactac cgagcggcgc ctgtcgcgga ttgcctaccg ggtggaaaag
     7561 cccgcgggtt gaccttgccg caattgcggc gtcaactatc tgcaactttt ctcgtcgccg
     7621 tgagagaaat ctctccgacg cgacaggaat agttgtgacg gcaccaaaca ccaataacga
     7681 gccgcgacgc ccatgaacaa gattcaggtc gacatgtacg ccgtggggct gggcgcggct
     7741 atcctcgttc agtatcgtct cgaagacgga aaggtcgtgc gcgtcctggc cgatggcggc
     7801 atgggccacg gcggttatcc cgtcgacggc gtgcatgacc gcatcgccga cgccttcacg
     7861 agtttcggtg acgacgagtc ccgcatcgat ctcatcatcg gtacgcacta cgacggcgat
     7921 cacctgaagg gcctggtccc catcgcggaa gacgattcat tgcagattgg cgaagtctgg
     7981 ttgccgccca tccgtgatga cagcgacgaa gtgccgggcc acatcagcga agattcgggc
     8041 ttccttgcgc acaagttttt cg
//
LOCUS       DATDEU010000102         9554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_19449, whole genome shotgun sequence.
ACCESSION   DATDEU010000102 DATDEU010000000
VERSION     DATDEU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19449"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="preA"
                     /locus_tag="VIV63_02410"
     CDS             complement(<1..837)
                     /gene="preA"
                     /locus_tag="VIV63_02410"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEY6123477.1"
                     /translation="MADLTTTFLGIKSPNPFWLASAPPTDKAYNVNRAFEAGWGGAVW
                     KTLGEDPPIVNVNGPRYGAIHSNDRRVIGFNNIELITDRPLGVNLEEIRQIKRDWPDR
                     ALIVSVMLPMNAQSWAKYVPMIEDTGCDGLELNFGCPHGMSERGMGSAVGQVPEYIQQ
                     AAAWCKQFARVPVIVKLTPNITNILPAAKAAKDGGADAVSLINTLNSIMRVDYDSLTI
                     YPSTDGKGSHGGYCGPAVKPIALHMVAEIARSRETRGLPISGIGGITDWRDAVDFMAL
                     GAT"
     gene            complement(839..2179)
                     /locus_tag="VIV63_02415"
     CDS             complement(839..2179)
                     /locus_tag="VIV63_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6123478.1"
                     /translation="MNNKPASPASHVQPGRLAEEKIAANFADMHPPLTPSEAIIDADR
                     CYFCYDAPCTTACPTGIDIPGFIQKIRSGNLKGSAHTILSENIMGGMCARVCPTEVLC
                     EEACVRNTHEDRPVRIGMLQRYATDPIFENDTPLFERAAATGRKVAIVGGGPAGLSCA
                     HRLAMLGHSPTVFEPRSKLGGLNEFGIAAYKTPDDFAAREVEYILSIGGIDVRTGVEL
                     GKDISLADMRENYDAVFLGFGLAGVNGLGLEGEQLEGVANAVDYISDLRQAPNKAALP
                     VGRKVVVIGGGMTAIDIAVQSKALGADEVTMVYRRGQENMNASGYEQEFAQTRGVTLR
                     TWSTPRKLLVSDGRVCGVEFESTQVAGETYVLESDMVFKAIGQKVLWDRLGDTAQILD
                     LKNHRILVDDERRTSLRGVWAGGDCVFGGEDLTVSAVQDGKLAALSIDQFLRSA"
     gene            complement(2181..3620)
                     /gene="hydA"
                     /locus_tag="VIV63_02420"
     CDS             complement(2181..3620)
                     /gene="hydA"
                     /locus_tag="VIV63_02420"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY6123479.1"
                     /translation="MSVLIKGGTVVTADRSYRADVLCSDGKIVDIGETLAAPARCEVV
                     DAGGQYVMPGGIDPHTHMQLPFMGTVSSDDFYSGTAAGLAGGTTMIIDFVIPNPKQRI
                     LDAHRQWREWALKSVADYSFHVAITWWDESVREDMGTLVREHGVNSFKHFMAYKNAIM
                     CDDETLVNSFSEALKLGAIVTVHAENGELVFRLQDEIYKRGITGPEGHPLSRPPEVEG
                     EAANRAIRIAEVLKVPVYLVHVSSRQALEAITRARNEGQRVFGEVLAGHLLLDDSVYR
                     DPDFTRAAAHVMSPPFRAPEHQVALWRGLQSGNLQTTATDHCTFCAEQKAAGKDDFRK
                     IPNGTAGIENRMEVLWHHGVNTGRLTMNEFVRVTSTNAAQIFNIYPRKGSVSVGADAD
                     IVVWDPAATKTISAKTHKQNIDFNIFEGITVKGGASHTVSGGKLVFAKGDLRVERGAG
                     KHVDRPAYPPYYDAVKKQLELSEPKPVRR"
     gene            complement(3628..5112)
                     /locus_tag="VIV63_02425"
     CDS             complement(3628..5112)
                     /locus_tag="VIV63_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019096710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEY6123480.1"
                     /translation="MTERAAKSRAVQVGEFYELEVGADLAASSRFNEDIAPTRVAQRT
                     WNEWHIAALWVGMAICVPTYTLGGVLTTYFGLSVGEALWAVFIANVIVLIPLTLNAFG
                     GTKFGIPFPVMLRSSFGIIGSNVPALIRALVACGWFGIQTLFGGIAVHLMLAAVSDTW
                     AQLGGVGEVIGFFLFWSANIWVVLRGSESIKYLEVFAAPLLMLVGVGLLVWAWPQASM
                     SELLARPPNRPAEASFWGYFFAGLTAMVGFWATLSLNIPDFSRFAKSQRAQIVGQVVG
                     LPVTMVLFAGLGVVMTAASAQLVGETVSDPITLIGKINSPFWVVASMFIIIIATISTN
                     TAANIVSPTNDFQNLAPRLINQKKGVLLTGFIGIALMSWELFKRMGWLQSNVSVDSLY
                     SNWLLGYSSLLGPIAGIMVVDYFVVRKQHYDLPGLYLNQGSYPAWNLAGFLALLVPVT
                     LTLVAITTGALQWFYTYGWFTGSLLGGLIYYLMNRGLMSRGSKE"
     gene            complement(5109..5984)
                     /locus_tag="VIV63_02430"
     CDS             complement(5109..5984)
                     /locus_tag="VIV63_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY6123481.1"
                     /translation="MSKLKCGLIQMGLKGDVSMGPEKIRDKMIEAHLPLIDKAGAAGV
                     QVLCFQEIFTQPYFCPSQDRKWYAAAEAVPDGYTTKLMQEYAKKYRMVMVVPVYEEAM
                     TGVYYNTAAVIDADGKYLGKYRKTHIPQVDPGFYEKFFFKPGDLGYPVFQTAYVKLGV
                     YICYDRHFPEGWRALALNGAEYIVNPSATVAGLSKYLWELEQPASAAANGVFIGAINR
                     VGKEAPWSDKMGEFYGSSYIVNPRGEIEAQASYDKDELLVHEIDLDMIRKVRDTWQFF
                     RDRRPDAYGPLVQGG"
     gene            6196..6663
                     /locus_tag="VIV63_02435"
     CDS             6196..6663
                     /locus_tag="VIV63_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123482.1"
                     /translation="MTATLPTDFDAFLFASVGSDSNGMPLTVVTLLARLGLDPWAEAA
                     ALAKLSLDSARQRLGERLEAAPNATATKAEVTTNATRLVALLHLVPIRKAASPQAPRR
                     DDESPLPAKLVKRAKEVPMAIYLLIGLILLLAGDWAFSTPQVETPMDTTSVSN"
     gene            6817..7269
                     /locus_tag="VIV63_02440"
     CDS             6817..7269
                     /locus_tag="VIV63_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY6123483.1"
                     /translation="MADDAATGADIYVYPAKGQNDRQLDRDRYECHGWASKQTGYDPS
                     QPPPATPLNVTLPPDHRGAVNGAVAGAVIGAAVSGHHDTAEGAVVGAVAGAVLGAATD
                     VSRAREAAGLASRQTAAEQAERARIEKQAYDYRRAIGACLEGRGYTVK"
     gene            7298..7930
                     /locus_tag="VIV63_02445"
     CDS             7298..7930
                     /locus_tag="VIV63_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123484.1"
                     /translation="MHRSPHKVLTGLFALLLLVVASQALADGPRGHGSRHYGSGNHGS
                     HAGYNRYSFSHHGSYSNYSYNNYWYRPYGPRYVVVAPPFGFGVSVLPPYYSTLWYGGS
                     PYYYADSTYYSWRPERREYVVTEKPRGEPETRVETGGSEEIFVYPRDGQTEEQQSTDR
                     YECHRWAVDKTGFDPTRPSGNVGGTEITTKRVDYRRAESACLEGRGYTVR"
     gene            7977..8843
                     /gene="purU"
                     /locus_tag="VIV63_02450"
     CDS             7977..8843
                     /gene="purU"
                     /locus_tag="VIV63_02450"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY6123485.1"
                     /translation="MTGAESTFSLLLVCPDRKGIVAAVAGFLSDHDASIVEAKQFNDQ
                     LTRRFYMRAVFTSVGPKFVAIEKLREAFAKLATPYEMNWQIEDTARRVRVLIAVSKLD
                     HCLQDLLFRWRSGVLPVDVVGVVSNHPDLRSLVEWHQIPFHHLPVTAETRPQQEAQIL
                     ELISRNQADLLVLARYMQILSNETCRTLSGRCINIHHSFLPSFKGARPYHQAYQRGVK
                     IIGATAHYVTPDLDEGPIIEQAVERVGHEHTPERFQEIGRDLEAIVLARAVRWHAERR
                     ILLDGQRSVILS"
     gene            8844..>9554
                     /locus_tag="VIV63_02455"
     CDS             8844..>9554
                     /locus_tag="VIV63_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY6123486.1"
                     /translation="MNRACMLLLVLPLSLGGCAQLQPRAELPMELATPTASSTTLDQT
                     FESLEVGHPGQSAFRLLVEGTEAFVARMQSARMASRSIDVQTYIWHADLTGKFLAWQL
                     LESADRGVRVRLLVDDMDARAKSAHFAALAAHPNIEVRAFNPFASRNGTLSLVSEGAR
                     NFKRINRRMHNKTWIADNRFAIVGGRNLGDEYFGASDEFNFVDLDFGMIGPVVRDASA
                     SFDRYWNSPSAYPIEMLDA"
BASE COUNT         1702 a         3065 c         3028 g         1759 t
ORIGIN      
        1 ggtggcgccc agggccatga agtcgacggc gtcacgccag tcggtgatgc cgccaatgcc
       61 cgagatcggt agtccgcgcg tctcgcgcga gcgcgcgatc tccgcgacca tgtgcagcgc
      121 aatgggtttg accgccggtc cgcagtagcc gccgtgcgaa cccttgccgt ccgtggacgg
      181 gtagatagtc agcgagtcgt agtcgacgcg catgatggaa ttcagggtgt tgatcagcga
      241 cacggcatcg gcgccgccgt ccttcgcagc cttcgcggcc ggcaggatat tcgtgatgtt
      301 cggcgtgagc ttcacgatca ccggcacgcg cgcgaactgt ttgcaccacg cggcggcctg
      361 ctggatgtac tccggcacct ggcccacggc cgaccccatg ccacgttcgc tcatgccgtg
      421 cggacagccg aagttcaact cgagaccgtc gcagccggtg tcctcgatca tggggacgta
      481 cttcgcccag ctctgtgcgt tcatgggcag catgacggac acgatgagtg cgcgatcggg
      541 ccagtcgcgc ttgatctgcc ggatttcctc gaggttcacg ccgagcgggc gatcggtgat
      601 gagttcgatg ttgttgaaac cgatgacgcg ccggtcgttg ctgtgaatcg caccgtaacg
      661 cgggccgttc acattgacga tgggcgggtc ttcaccgagc gtcttccaca ccgcgccgcc
      721 ccagccggcc tcgaacgcgc gattgacgtt gtacgccttg tcggtgggcg gcgcggaggc
      781 gagccagaac gggttggggg acttgatgcc gagaaaggtg gtggtgagat cggccatgtc
      841 acgcactcct cagaaactgg tctatcgaca gcgcggcgag ctttccgtcc tgaacggccg
      901 acaccgtcag gtcttcaccg ccaaaaacgc agtctccgcc agcccacacg ccgcgtagcg
      961 atgtgcggcg ctcgtcgtcg acgagaatcc ggtggttctt gagatccaga atctgcgcgg
     1021 tgtcgccgag gcggtcccag agcactttct ggccgatggc cttgaagacc atgtcggatt
     1081 cgagcacgta agtttcgccg gcgacttgcg tcgattcgaa ctccacgccg cagacgcggc
     1141 cgtcgctcac gagcagtttg cgaggcgtcg accaggtgcg cagcgttacg ccgcgagtct
     1201 gcgcaaattc ctgttcgtag ccgctggcgt tcatgttctc ctggccgcgc cggtagacca
     1261 tggtcacttc gtcggcgccc agcgccttgc tctgcacggc gatgtcgatg gccgtcatgc
     1321 cgccgccgat cacgacgacc tttcgcccca cgggcaatgc agccttgttg ggggcctggc
     1381 gcaagtcgga gatgtagtcg acggcattcg ccacgccttc gagttgttcg ccctcgaggc
     1441 cgaggccgtt gacgccggcg aggccgaagc cgagaaacac cgcgtcgtag ttctcgcgca
     1501 tgtcggcgag tgaaatatcc ttgcccagct cgacgccggt gcgcacgtcg atgccgccga
     1561 tgctcaggat gtattccacc tcgcgcgccg cgaagtcgtc gggagtcttg taagccgcga
     1621 tgccgaattc gttgaggccg cccagcttgc tcctgggctc gaacaccgtg gggctgtggc
     1681 ccagcatcgc gagtcgatgc gcgcagctca ggcccgcggg tccgccaccg acgatcgcca
     1741 ccttgcggcc ggtggccgcg gcgcgctcga acagcggcgt gtcgttctcg aagatgggat
     1801 cggtggcata tcgctgcaac atgccgatgc gcaccggccg gtcttcgtgc gtgttgcgca
     1861 cgcaggcttc ttcgcacagc acttcggtcg ggcacacgcg ggcgcacatg ccgcccatga
     1921 tgttttcgct gaggatggtg tgggccgagc ccttgagatt gccgctgcgg atcttctgga
     1981 tgaagccggg gatgtcgatg cccgtggggc acgcggtggt gcagggcgcg tcgtagcaga
     2041 agtagcagcg gtcggcgtcg atgatggctt ccgacggcgt cagcggcgga tgcatgtccg
     2101 cgaagttggc ggctatcttc tcttcggcga gtcggccggg ctggacgtgg ctggctgggc
     2161 tggctggctt gttgttcaca ctaccgcctg acgggtttcg gttcgctgag ctctaactgc
     2221 tttttgacgg catcgtagta cggagggtag gcggggcggt ccacgtgttt gcccgcgccg
     2281 cgttcgacgc gcagatcgcc tttcgcgaac acgagcttgc cgccgctcac ggtgtgcgac
     2341 gcgccgcctt tgaccgtgat tccttcgaaa atgttgaagt cgatgttctg cttgtgagtt
     2401 ttggcggaaa tcgtcttggt cgcggccggg tcccacacca cgatgtccgc atccgcaccc
     2461 accgaaacgc tgcccttgcg cggatagatg ttgaagatct gcgccgcgtt cgtggacgtg
     2521 acgcgcacga attcattcat ggtgaggcgg ccggtattca cgccgtggtg ccacaacact
     2581 tccatgcggt tctcgattcc ggcggtgccg ttgggaatct tgcggaaatc gtccttgccc
     2641 gctgccttct gttccgcgca gaacgtgcag tggtcggtgg ccgtggtctg cagatttccc
     2701 gactgcagcc cgcgccagag cgccacctgg tgctcgggcg cgcggaatgg cgggctcatg
     2761 acatgcgcgg cggcgcgcgt gaagtccggg tcgcgataca ccgagtcgtc cagcaacaga
     2821 tggcccgcca gcacttcacc gaacacgcgc tggccttcgt tgcgcgcgcg cgtgatcgcc
     2881 tcgagcgcct ggcgcgacga gacgtgcacc aggtagacag gcaccttgag cacttcggcg
     2941 atgcggattg cgcgattcgc ggcttcgccc tccacttccg gcggacgcga cagcggatgg
     3001 ccctccggcc cggtgatgcc gcgcttgtag atctcatcct gaagccggaa caccagctcg
     3061 ccgttttcgg cgtgcacggt gacgatggcg ccgagcttca gcgcctcgga gaagctgttc
     3121 accagcgttt cgtcgtcgca catgatggcg ttcttgtacg ccatgaaatg tttgaagctg
     3181 ttgacgccgt gttcgcgcac cagcgtgccc atgtcttcgc ggacgctttc atcccaccag
     3241 gtgatggcga catggaagga atagtcggcg acggatttca gcgcccactc gcgccactgt
     3301 cgatgcgcgt cgaggattcg ctgcttcgga ttcgggatga cgaaatcgat gatcatcgtc
     3361 gtgccgcccg cgagtcccgc ggccgtgccc gaatagaagt catcgctcga caccgtgccc
     3421 atgaacggca gctgcatgtg cgtatgcggg tcgatgccgc ccggcatcac gtactggccg
     3481 ccggcgtcga cgacttcgca gcgcgcaggc gctgcgagtg tttcgccgat gtcgacgatc
     3541 ttgccgtccg aacacaatac gtcggcgcgg tatgaccgat ccgccgtgac gaccgtgccg
     3601 cccttgatga gtacgctcat gcgaacctca ctccttgctg ccgcggctca tcaggccccg
     3661 gttcatcaaa taatagatga gcccgcccag taacgaccca gtgaaccagc cgtaagtgta
     3721 gaaccactgc agcgcacctg tcgtgattgc gacgagggtg agtgtgacgg gcacgagcaa
     3781 tgccaggaat cccgcgagat tccaggccgg atacgagccc tggttgaggt acagccccgg
     3841 cagatcgtaa tgctgctttc gcaccacgaa atagtcgacg accatgatgc cggcgatggg
     3901 gcccagcagg ctcgaatagc cgagcagcca gttcgaatac aggctgtcga cgcttacgtt
     3961 cgactggagc cagcccatgc gcttgaacag ctcccagctc atgagcgcga tgccgatgaa
     4021 gcccgtgagc aacacgcctt tcttctggtt gatcagccgc ggcgcgagat tctgaaagtc
     4081 gttcgtgggc gagacgatgt tggccgcggt gttggtcgag atggtggcga tgatgatgat
     4141 gaacatcgac gccacgaccc agaacgggct gttgatcttg ccgatcagcg tgatcggatc
     4201 ggacaccgtc tcgccgacga gttgggcgga cgctgcagtc atgaccacgc ccaggcccgc
     4261 gaacaagacc atcgtcactg gcaatcccac gacctggccg acgatctgcg cgcgctgcga
     4321 tttggcgaag cggctgaaat ccggaatgtt cagcgacaag gttgcccaga aacccaccat
     4381 cgcggtcagg cccgcgaaga agtagcccca gaacgaggct tccgccggcc ggttcggcgg
     4441 acgcgccagc aactcgctca tcgacgcctg aggccacgcc cacaccagca ggccgacacc
     4501 cacgagcatc aacagcggcg ccgcgaacac ttcgaggtac ttgatggatt ccgagccgcg
     4561 caggaccacc cagatgttgg cggaccagaa gaggaagaac ccgatgactt cgccgacccc
     4621 accgagttgc gcccaggtgt ccgacaccgc ggccagcatc aggtgcacgg caatgccgcc
     4681 gaacagggtc tggatgccga accagccgca ggccacgagc gcgcggatca gcgccggtac
     4741 attcgagccg atgatgccga acgaggagcg cagcatcacg ggaaacggaa tgccgaactt
     4801 cgtgccgccg aacgcgttga gcgtcagcgg aatcagcacg atcacgttgg cgatgaacac
     4861 cgcccacaat gcctcgccca ccgacaggcc gaagtaggtg gtcagcacgc cacccagcgt
     4921 gtaggtaggc acgcagatcg ccatgcccac ccacagggcg gcgatgtgcc actcgttcca
     4981 ggtgcgctgg gcgacacgtg tgggcgcgat gtcttcgttg aagcgcgaac tcgcggcgag
     5041 atcggcgccg acttcgagct cgtagaattc gccgacttgt accgcgcggc ttttcgcggc
     5101 ccgctccgtc atccgccttg caccagcggg ccgtaggcgt cggggcgccg atcgcggaag
     5161 aactgccacg tatcgcggac cttgcggatc atgtcgaggt cgatttcgtg caccagcagc
     5221 tcatctttgt cgtaactcgc ctgtgcctcg atctcgccgc gcgggttgac gatgtagctc
     5281 gagccgtaga actcgcccat cttgtcggac cacggcgctt ccttgcccac gcggttgatc
     5341 gcgccgatga acacgccgtt cgccgcggcg gaagcgggct gttcgagttc ccagagatac
     5401 ttgctgagtc ccgcgaccgt ggccgatgga ttgacgatgt actcggcgcc gttcagcgcg
     5461 agcgcgcgcc agccttcggg gaagtggcgg tcataacaga tgtacacgcc taacttcacg
     5521 tacgcggtct ggaacaccgg gtagccgaga tcgccgggct tgaagaagaa tttttcatag
     5581 aagccggggt cgacctgcgg aatgtgcgtc ttccggtatt taccaaggta cttgccgtcg
     5641 gcgtcgatga cggcggcggt gttgtagtac acgccggtca tcgcttcttc gtagaccggg
     5701 acgaccatga ccatgcggta cttcttcgcg tactcctgca tgagcttcgt ggtgtagccg
     5761 tcgggcaccg cttcggcggc cgcgtaccac ttgcgatcct ggctcggaca gaaatacggc
     5821 tgcgtgaaga tttcctggaa gcacaacacc tgcacgccgg cggcgcctgc cttgtcgatg
     5881 agaggcagat gcgcctcgat catcttgtcc cggattttct cgggacccat cgacacgtcg
     5941 cccttcaacc ccatctggat caatccgcac ttgagcttgc tcacccggaa actcccgcct
     6001 tgagatgacg ggatcataaa aacacagaac ggaaaaaatg cggagcgggt gcggcagcgg
     6061 ggcgatccag ttgagaaccg ttcttattaa cccgcgtact cttctatatg ggccaggcaa
     6121 gccatgggat tcgggggtac cgaccggtac agaagcgtac ggatgccgcg aaccgattca
     6181 tgcgatcttg tgaccatgac tgccacgctc ccgacggatt tcgacgcttt cctgttcgcc
     6241 tcggtcggtt ccgattccaa cggcatgcca ctcactgtgg tgacgctgct cgcgcggctg
     6301 ggtctcgatc catgggccga ggctgcggcg ctcgccaagc tgtcgctcga ttccgcgcgg
     6361 cagcggctgg gcgagcggct cgaagccgcg cccaacgcga ccgcgacgaa agccgaggtg
     6421 accaccaatg caacgcgcct ggtcgcgctg ttgcacctgg ttccgattcg caaagcggct
     6481 tcgccacagg cgccccggcg cgacgatgaa tcgccgttgc ccgccaagct ggtgaagcgc
     6541 gcaaaggaag tgccgatggc aatctacttg ctgatcggcc tcatcctgtt gctcgcgggc
     6601 gattgggcct tctcgacacc gcaggtggaa acgccgatgg ataccaccag cgtttcgaac
     6661 tagcagatct ggtcgccggt cgttcgctga aacttgccca ccgcgcgacg gtcagatcag
     6721 gcagtaaagc ggagtcgtcc aatgcgtgta aatcaacgcc gggcatccgg ccgccctctc
     6781 ggtttgacgc tggcactgtc attcgctgcg gccgccatgg cggacgacgc ggcgaccggc
     6841 gcggatatat acgtgtatcc cgccaaaggg caaaacgacc ggcaactcga ccgcgatcgt
     6901 tacgaatgcc acggctgggc gtccaaacag actggttacg accccagcca gccgccgccg
     6961 gcgactcctt tgaatgtgac cctgccgcct gatcaccgcg gcgcggtcaa tggtgcggtc
     7021 gcgggcgccg tcatcggcgc tgcggtttcc ggtcaccacg ataccgccga aggcgcggtg
     7081 gtcggcgccg ttgccggggc ggtgctcggc gcggccacgg atgtctcgcg cgcccgggaa
     7141 gccgcgggtc tcgcatcgcg gcaaacggcg gcggaacaag cggaacgagc aaggatcgag
     7201 aagcaggcat acgattaccg gcgtgccatc ggcgcctgcc tggaggggcg cggatacacg
     7261 gtcaaatagt tttcgtcgcc ggaccgcgga ggttcgcatg catcgctcac cgcacaaagt
     7321 gctgaccggc ctgttcgcgt tgctgctcct ggtggttgcc tcccaggcgc tcgccgacgg
     7381 accgcggggg catggctcgc ggcactacgg gtcggggaac cacggctcgc acgctggcta
     7441 caaccgctac agcttctcgc accacggttc ctactctaac tacagttaca acaactactg
     7501 gtaccggcct tatggcccgc gctacgtggt cgtagcgccg ccgttcggtt tcggcgtgag
     7561 cgtgctgccg ccgtactaca gcacgctctg gtatggcggc tcgccttact actacgccga
     7621 cagcacctat tactcctggc ggccggagcg ccgcgaatac gtggtgaccg agaaaccgcg
     7681 cggcgagccc gagacgcgcg tcgagaccgg cggcagcgaa gagatcttcg tctacccgag
     7741 agacgggcag accgaagagc agcagtccac cgaccgctac gaatgccatc gctgggccgt
     7801 ggacaagacc ggattcgatc cgacgcggcc gtcgggcaat gtcggcggca ctgaaatcac
     7861 cacgaagcgc gtcgactatc gccgcgccga atcggcctgt ctcgagggcc ggggctacac
     7921 cgtccgatag tcaggcgacg gaatcgctac actccgcggc aaccagcgga gtgagcatga
     7981 ccggggccga atcgacgttc tcgttgttat tagtgtgtcc cgaccgcaag ggcatcgtcg
     8041 ccgcggtcgc cggattcctg tccgaccacg acgcgtccat cgtcgaggcc aagcagttca
     8101 atgaccagct gacacgccgc ttctacatgc gcgcggtgtt cacgtcggtg ggcccgaagt
     8161 tcgtggccat cgagaagctg cgcgaagcat tcgcgaagct cgcgacgcca tacgagatga
     8221 actggcagat cgaagacacc gcccggcggg tccgggtcct catcgcggtg tcgaaactgg
     8281 accattgcct gcaggacctg ctgtttcgct ggcgatcggg tgtgttgccc gtcgacgtcg
     8341 tgggtgtcgt ctccaaccac ccggacctgc gaagcctcgt cgaatggcat cagatcccgt
     8401 tccatcatct gccggtcacc gccgagacgc ggccacagca ggaagcgcag atcctcgagc
     8461 tgatctcgcg caatcaggca gaccttctcg tgctcgcgcg ttacatgcag atcctctcga
     8521 acgagacctg ccgcaccctg agcggccgct gcatcaacat ccatcattcg ttcctgccca
     8581 gcttcaaggg cgcgcggcct taccaccagg cctatcaacg tggcgtgaag atcatcggcg
     8641 ccactgccca ttacgtcacc cccgatctcg atgaggggcc gatcatcgaa caggccgtcg
     8701 agcgcgtcgg ccacgaacac acgcccgagc gcttccagga aatcggccgt gatctcgagg
     8761 cgatcgtgct ggcccgcgcg gttcgctggc atgccgagcg gcgaatcctg ctcgacgggc
     8821 aacgcagcgt aatcctgagc tgaatgaacc gcgcgtgtat gctgctgctg gtgctgccgc
     8881 tctcgctggg cggttgcgcg caattgcaac cgcgggccga actacccatg gaactcgcta
     8941 ctccgaccgc cagcagcacg accctcgatc agacgttcga gtcactggaa gtcggtcatc
     9001 cgggacagtc ggcattccgg ttgctcgtcg aaggtaccga ggccttcgtc gcgcgcatgc
     9061 aaagcgcgcg catggcctcg cgcagcatcg atgtgcagac ctacatctgg cacgcggacc
     9121 tcaccggaaa attcctcgcc tggcagctgc tcgaatcggc ggaccggggt gtgcgcgtcc
     9181 gcctcctggt cgatgacatg gacgcgcgcg ccaagagcgc gcatttcgcc gcgctggctg
     9241 cgcatccgaa catcgaggtt cgtgcgttca atccgttcgc atcgcgcaac ggcacgctga
     9301 gcctggtgag tgaaggcgcc cgcaatttca agcgcatcaa tcggcgcatg cacaacaaga
     9361 cctggatcgc cgacaaccgg ttcgcgatcg tcggtggccg caatctcggt gatgaatact
     9421 tcggggcgag cgacgagttc aattttgtcg atctcgattt cggcatgatc ggcccggtcg
     9481 tgcgcgacgc gtcggcgtcc ttcgaccgat actggaattc gccatcggcc tatcccatcg
     9541 agatgctcga cgcc
//
LOCUS       DATDEU010000103         3246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2062824, whole genome shotgun sequence.
ACCESSION   DATDEU010000103 DATDEU010000000
VERSION     DATDEU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062824"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /gene="ccoG"
                     /locus_tag="VIV63_02460"
     CDS             complement(<1..1155)
                     /gene="ccoG"
                     /locus_tag="VIV63_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEY6123487.1"
                     /translation="MAACNPQAAGDSLYLAEAKVYPREVSGRFARLRTLSVWVLLGIF
                     YLLPWLQWGGRQAVLFDLPARKFFILGLVFWPQDFIFLTWLLIIAALALFFFTAIGGR
                     LWCGYACPQTVWTETFLWMERFAEGDRGARLKLDKAPWSPVKFGRKSLKQLLWITFAL
                     WTGFTFVGFFTPIRTLSGEVLGLGLGPWETFWILFYGFATYGNAGYLREQVCKYMCPY
                     ARFQSAMFDRDTLVITYDEKRGEPRGARRRGTDARTQGKGDCIDCTLCVQVCPTGIDI
                     RKGLQIECIACAACIDVCDDVMDKMGSPRGLIRYTTTHSLEHLPTRILRPRTLIYGSI
                     LGLLMVGFVVAVAMRSPVSLDVIRDRNALFKLTDDGNVDNVYTIRILNKSE"
     gene            complement(1156..1431)
                     /locus_tag="VIV63_02465"
     CDS             complement(1156..1431)
                     /locus_tag="VIV63_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123488.1"
                     /translation="MSASRSDESRDIGTRSRAFGVIVWSAFLSAALATMLCFALVDPL
                     AIAAGEPPHWWTDRVHVYGVGFFFFWLIGVVAATLCWQLATAPRRDP"
     gene            complement(1412..2341)
                     /gene="ccoP"
                     /locus_tag="VIV63_02470"
     CDS             complement(1412..2341)
                     /gene="ccoP"
                     /locus_tag="VIV63_02470"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEY6123489.1"
                     /translation="MTSAHSVFIIALTLANIAGALWLLWWTRRSHGEPGLTTETTGHV
                     WDGDLRELNNPLPRWWLWLFIISVAFGGVYLALYPGLGSNQGTLQWTSRAEHDAAARA
                     NAARIEKTLAVYSSRDVADLALDSAALNVGRNLFLNNCATCHGSDGGGAPGFPNLTDK
                     DWLWGGAPETVRATIANGRAGIMPPWGDALGVGGVEDMLAYVYSLQGRELPTGNRLAG
                     QRKFGELCAACHGDDGRGKVEMGTPNLTDGVWLHGGALASIRDSISHGRSGTMPAQLD
                     RLGETRVKLLAAYVLSLGRTEEPEVVADVRLAQ"
     gene            complement(2338..2487)
                     /locus_tag="VIV63_02475"
     CDS             complement(2338..2487)
                     /locus_tag="VIV63_02475"
                     /inference="COORDINATES: protein motif:HMM:NF017366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEY6123490.1"
                     /translation="MDIGVFRGLVTAALLGLFVWLVVWSWSRKRQADFDAAAQLPLED
                     EGSRS"
     gene            complement(2493..3098)
                     /gene="ccoO"
                     /locus_tag="VIV63_02480"
     CDS             complement(2493..3098)
                     /gene="ccoO"
                     /locus_tag="VIV63_02480"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEY6123491.1"
                     /translation="MNHEAVEKKVGLLGVLIAIVISIGGLVEIVPLYFQQTVTTPSPG
                     IAPYDALRLAGRDIYIREGCYNCHSQMIRPLRAETERYGHYSLAGESVFDHPFQFGSK
                     RTGPDLARVGGRYSDEWHRAHLNNPRDVVPESNMPAYVWLANSPVDAAGTPDKMRALR
                     KVGVPYTDEDIAGAEEAVRGKTDMDALVAYLQGLGTAAKNW"
BASE COUNT          624 a         1054 c         1014 g          554 t
ORIGIN      
        1 ctcgctcttg ttcaggatgc ggatcgtgta gacgttgtcg acgtttccgt cgtcggtgag
       61 tttgaaaagc gcattgcggt cccggatcac gtccagcgac accggcgagc gcattgccac
      121 cgccaccacg aagcccacca tgagcagccc caggatcgac ccatatataa gagtgcgcgg
      181 ccgcaggatc ctcgtgggca ggtgctcgag cgaatgcgtg gtcgtatagc ggatgaggcc
      241 gcgcggcgag cccatcttgt ccatgacgtc gtcgcagacg tcgatacagg ccgcgcaggc
      301 gatgcattcg atctgcagac cctttcttat gtcgatcccg gtgggacaga cctgcacgca
      361 caatgtgcag tcgatgcagt ccccttttcc ctgcgtgcgc gcatcggtgc cccgccgccg
      421 agcgccccgg ggctcgccgc gtttctcgtc gtaagtgatc acgagagtgt cgcggtcaaa
      481 catggcgctc tgaaaacgcg catacggaca catgtacttg cacacctgtt cgcgcaggta
      541 gccggcattg ccgtacgtgg caaagccgta gaacaggatc cagaaggtct cccacggtcc
      601 gaggccgagg ccgagcactt cgcccgaaag cgtccggatg ggcgtgaaga agcccacgaa
      661 agtgaacccg gtccacagag cgaacgtgat ccacaggagt tgtttgagcg acttgcgccc
      721 gaacttcacc ggactccacg gcgccttgtc gagcttgagc cgcgcgccgc gatcaccctc
      781 ggcaaatcgt tccatccaca ggaaggtttc cgtccacacg gtttgcgggc aggcgtagcc
      841 acaccatagt cgtccaccga tcgccgtgaa gaagaacaat gcgagcgcgg cgatgatcag
      901 gagccacgtg aggaatatga aatcctgcgg ccagaacacc agcccaagga tgaagaactt
      961 gcgtgccggc aggtcgaaca acacggcctg acggccgccc cactgcagcc acggcaatag
     1021 atagaagatg ccgagcaaca cccacaccga aagcgtgcgc agacgcgcga atcttccgct
     1081 gacctcgcgt ggatagacct tggcctccgc gagatagagc gagtctcccg cagcctgcgg
     1141 gttgcacgcg gccatttatg ggtcccggcg gggcgcggtt gcgagctgcc agcacagcgt
     1201 ggcagcgacc acgcctatga gccagaaaaa gaagaaaccg accccgtaca cgtgaacgcg
     1261 gtcagtccac cagtgaggag gttcccccgc cgcgatggcc agggggtcca cgagtgcgaa
     1321 acacaacatg gtggccagcg cggcggacag aaatgcgctc catacgatca cgccgaacgc
     1381 gcggcttcgc gtgccgatat cccggctttc gtcactgcgc gaggcggaca tcggccacga
     1441 cctcgggctc ttccgtcctg ccgagcgaga gcacgtacgc cgcaagtagt ttgacccgcg
     1501 tctcgcccag gcggtcgagc tgcgcgggca tggtgcccga gcggccatgc gagatgctgt
     1561 cgcgaatcga tgccagcgcg ccgccgtgca gccagacgcc atcggtgaga ttgggcgtac
     1621 ccatctcgac ttttccacgg ccatcgtcgc cgtggcaggc ggcgcagagc tcgccgaatt
     1681 tccgctgccc ggccagcctg ttgccggtcg gcagttcgcg tccctgcagc gaatagacgt
     1741 aagcgagcat gtcttccact ccgcccacgc ccaacgcgtc gccccagggc ggcatgattc
     1801 ccgcccggcc attggcaatc gtggctcgca cggtttccgg agcgccgccc cagagccagt
     1861 ccttgtctgt cagattgggg aagcccggtg cgccgccgcc atctgatccg tggcaggtgg
     1921 cgcagttgtt gagaaacagg ttgcggccca cgttgagcgc agccgaatcg agcgccaggt
     1981 cggcgacgtc gcgggatgaa tagaccgcca gcgtcttttc gatgcgcgcc gcattggcgc
     2041 gcgcggccgc gtcgtgttcc gcgcgtgaag tccactgcag ggttccctga ttggagccca
     2101 gccccggata caacgccaga taaactccgc cgaaggcgac cgagatgatg aacagccaca
     2161 gccaccagcg cggcagcgga ttgttcagct cccgcagatc gccatcccag acgtgtcccg
     2221 tggtctcggt agtgagacct ggttcgccgt gtgatcggcg cgtccaccac agcaaccaca
     2281 gcgcgccggc gatattcgca agcgtgaggg caatgatgaa aacgctgtgc gccgaggtca
     2341 tgagcgtgac ccctcgtcct cgagcggcag ctgcgcagcg gcgtcgaagt cggcctggcg
     2401 tttcctgctc caggaccaga ccaccagcca gacgaacaag ccgagcagcg cggccgtcac
     2461 cagtcctcga aatactccga tatccatcgt gctcaccagt tctttgcggc cgtgccaagg
     2521 ccctgtagat aggcaacgag tgcgtccata tccgtcttgc cgcggacggc ttcctcggcc
     2581 ccggctatgt cctcatccgt gtacggcaca cccaccttgc gcagggcgcg catcttgtcc
     2641 ggcgtaccgg cggcgtccac cggtgaattc gccagccaga catacgccgg catgttggat
     2701 tccgggacca cgtcgcgcgg attgttcaga tgcgcgcgat gccattcatc gctgtaccgg
     2761 ccgccgacgc gcgcgagatc cggccccgtg cgcttggaac cgaactggaa tggatggtcg
     2821 aacacgcttt caccggccag tgaatagtgg ccgtaacgct cggtctcggc gcgcaagggt
     2881 cggatcatct gcgagtggca gttgtagcaa ccctcgcgga tatagatgtc gcgcccggcg
     2941 agtcgcagcg cgtcatatgg cgctatgccc ggtgacggcg tcgtcacggt ctgctggaag
     3001 tagagcggca cgatttcgac caggccgcca atgctgatca cgattgcaat cagcacgccg
     3061 agcaggccga ctttcttttc tacagcttca tggttcatga gtggtacctg tcaggcgggc
     3121 tgcacgatgg gagagtcgac cgtcgtactg cgggccgcga tggtcttcca gacgttgtat
     3181 gccatgagga acatgccacc gagcacgagc gcgccgcccg cgaggcgtac cgcgtagtaa
     3241 gggtag
//
LOCUS       DATDEU010000104         6706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2062857, whole genome shotgun sequence.
ACCESSION   DATDEU010000104 DATDEU010000000
VERSION     DATDEU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062857"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VIV63_02485"
     CDS             <1..488
                     /locus_tag="VIV63_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123492.1"
                     /translation="FMASTSSAVGLNTVVSPIYQVGNGATVKLGHTVIANTNYNRLFA
                     GGTYIASCAAPDMAPASGQRTVSAENYVGGLTLYVTIPAYHPAYVNMPGFNAAATRGM
                     RINCVYNWSSRAVEGGYSVGVGGISFQTGNGEASEGSSQPFSMNVPALADEDEWTSCY
                     P"
     gene            512..1138
                     /locus_tag="VIV63_02490"
     CDS             512..1138
                     /locus_tag="VIV63_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123493.1"
                     /translation="MKLFKRLIVVSLVGIAAVGGHAWSGGCSPIVIDLDNKGIDLGKA
                     GVGVYFDVNADGVRDHVQWVRHGGDEAFLAIDRSGNGRIDDGSELFGVGTPMILESRN
                     APNGFVGLAQYDSRQLGGNDDGDITAADAIWPQLRLWIDLNADGISTSDEMRTLQSAG
                     LTSLDTIPRIRKHVDDAGNVIPYWAWADKRAKPGRALMADVFFLVLPK"
     gene            complement(1145..1564)
                     /locus_tag="VIV63_02495"
     CDS             complement(1145..1564)
                     /locus_tag="VIV63_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6123494.1"
                     /translation="MPAPKSLTRILDELRASGPARMRRQIRVPDAAPALARLLGNCKT
                     LAVEERANGVAFRLEGATEAAVPFAMTCVLHQPADDSPLTRAEQTVAELLCEGRTLAQ
                     IAHLRGVSANTIKSQVRQVFRKLNVESRVALVRKMCP"
     gene            complement(1649..2197)
                     /locus_tag="VIV63_02500"
     CDS             complement(1649..2197)
                     /locus_tag="VIV63_02500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY6123495.1"
                     /translation="MPKQIGYVVGSLRKESINRKLANALIKLGPPDFTFKELRIGDLP
                     LYNQDDDKAQAPEVQRLKTELRAVDAVIFVTAEYNRSIPGVLKNAIDHASRPYGQSAW
                     AGKPAGVIGASVGAISTAVAQLHLRTILAYLDMPTLGQPEAYIQVRDGFFDDAGNFAS
                     AETRKFLHGWMDKYVAWVKKLT"
     gene            2312..2656
                     /locus_tag="VIV63_02505"
     CDS             2312..2656
                     /locus_tag="VIV63_02505"
                     /inference="COORDINATES: protein motif:HMM:NF018991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HEY6123496.1"
                     /translation="MHKRGLQILFTFILLSLTAYNLWVSAQQPVWEWGGLTTSPDNLW
                     TIATLIDAYYGFITFYVWVLWKETRALPRVSWFVAIMLLGNIAMSVYVLRQLARLGPS
                     DSMGTLLSARNA"
     gene            2661..3125
                     /locus_tag="VIV63_02510"
     CDS             2661..3125
                     /locus_tag="VIV63_02510"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY6123497.1"
                     /translation="MRDFLQKRLVNPLLALLRQGVTPRDLAMSVALGTSLGLVPVLGV
                     STALCALAALLLRLNMPAIQIVNYLLTPLQLVLIIPWLRFGEMLAGAPRFPVTLDSGL
                     ALLSHGAINAIQVLGLAILHATFGWLVLAPVLAFVLYRGLEPAFRHLRPEAS"
     gene            3122..4174
                     /locus_tag="VIV63_02515"
     CDS             3122..4174
                     /locus_tag="VIV63_02515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6123498.1"
                     /translation="MTSLAMQLLERDLVPDFLIRRRIRTLLAARLREEDQRDPAKQQQ
                     RLIDFIRELEVSPVAIETAAANQQHYEVPTEFYTRVLGKHLKYSSCLYPHPDAAPAEN
                     LDAAEARMLGLTCQRARLDNGQQILELGCGWGSLSLWMAANYPGSRITAVSNSRTQRR
                     HIEHQAAARGLTNLEVITCDVNQLSFPDDRRFDRVVSVEMFEHMRNYDTLLARVASWM
                     APGATLFVHIFTHREYAYPFEVRDESDWMARHFFTGGIMPSDDLLLYFQRDVRLIEHW
                     QVPGWHYQLTSEAWLSNMDRNRAELMPLLARTYGEPQALRWWVYWRVFFMSCAELWGY
                     ADGREWLVSHYLFEKR"
     gene            4196..5635
                     /locus_tag="VIV63_02520"
     CDS             4196..5635
                     /locus_tag="VIV63_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6123499.1"
                     /translation="MAADNSFRWLTATALCFASFLQAQPAFAVTDADRVAVYKEFRLN
                     YDAKKYVEAQPLAERLVALTEEQYGAEELALTNPLTNLATVHYKLGHYPAAIENYQRT
                     LRILQAKSTIADKQQIRPLHGLGVSFMGANDPESAVVALKRAADLSRNTDGLFNINQV
                     EFIDALIDAYEASGRYPEAEKEALYAMRVEEAAYGRASIKLLPRFDKLARWYEGARRY
                     TSERNIYERALSVLAKSAPENDLRRVGPLRGIARSFRLEAFYGVEGADSGSTFNSGNA
                     GAPVFSDGTQQRRGESALGTALDIIDRNAPVNEQMRGEVLTDLGDWYLIANALRRAYD
                     TYAEAWKAFAKASNTKPLERPRVLAYRPSVSSIDRSQLDPAEAVIKVVELRFTVDRDG
                     RIDNVTSPTTDVPESIVRNSMSSMRRSRYAPRIENGAAVPTADVVFHERVLVKITESG
                     SETSSGKEKAAEKPPEAAPEPSTPPPAGR"
     gene            complement(5532..6329)
                     /locus_tag="VIV63_02525"
     CDS             complement(5532..6329)
                     /locus_tag="VIV63_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123500.1"
                     /translation="MNVNRISQLSIPFAAFTLLLGGCTAVAVRPEGDLPKAIIVPTPA
                     KVGVVVTTEAANYVHKESRASVNYEAQLGESHKHLVEEIFRAEFTDAKMFETVDAARL
                     EPGILAIFEPRIEQFSFASARETGGVYTAVTIRYQIFIYAPNGELVDTLTLTGYGSGP
                     APKIGNGEEELGIAAYAAMRDAAAKFLTQFPTLDLAKPLLASQALQPKSRPEPGSPEA
                     AAAIADMTIEAVPINDQPVVVSTAPEPPPAASPPLSPSQMTSQIRTQ"
     gene            complement(6334..>6706)
                     /locus_tag="VIV63_02530"
     CDS             complement(6334..>6706)
                     /locus_tag="VIV63_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123501.1"
                     /translation="KPFSDSSPVEGIGAAVQGWWSADKKISVYVIGDDFTGPSVEAAL
                     QAVRKYNPMDRQGRRKIRIHAIGMPAGASSPPRINQRFSVLMRAMCDENEGTFVGLTI
                     GGASCAIKVDVFGQPRCITDG"
BASE COUNT         1198 a         2167 c         2076 g         1265 t
ORIGIN      
        1 tgttcatggc cagcacgtcg tccgccgtcg gcctcaatac ggtcgtttcg ccgatttatc
       61 aggtgggcaa tggcgccacc gtcaagctgg gccacacggt catcgcgaac actaactaca
      121 accgtctgtt cgccggaggg acctacatcg ccagctgtgc ggccccggac atggcgccgg
      181 ctagcggcca gcgaaccgtc tcggcggaaa actatgtcgg aggcctcacg ctctatgtca
      241 cgattcctgc ctatcatccc gcgtacgtga acatgcccgg attcaacgcg gcggccactc
      301 gcggcatgag aatcaactgc gtgtacaact ggagttcgcg cgcggtcgag ggcggttact
      361 ccgtcggagt cggcggcatc agcttccaga ccggcaacgg cgaagcttcg gaaggaagtt
      421 cgcaaccgtt cagcatgaac gtccccgccc tcgccgacga agacgaatgg accagctgct
      481 atccgtaaga ctcttaccaa ggacggtaat catgaaactc ttcaaacgat tgattgtagt
      541 tagtttggtc ggcatcgctg ccgtgggcgg ccacgcatgg agcggcggct gcagccccat
      601 cgtcatcgat ctcgacaata agggcatcga cctcggcaag gccggagtcg gcgtctactt
      661 cgacgtcaac gccgacggcg tgcgcgatca cgttcaatgg gttcggcacg gcggcgacga
      721 ggcatttctc gccatcgatc gcagtggcaa tggccgcatc gacgacggct cggagttgtt
      781 tggtgtcggc acgcccatga tcctcgaaag ccgcaatgcg cccaatggct tcgtgggtct
      841 tgcgcaatac gattcgcgcc agcttggcgg caatgacgac ggcgacatca ccgcggcgga
      901 cgccatctgg ccacagttgc gcctgtggat agacctgaat gcggacggca tttccaccag
      961 cgacgagatg cgcacgcttc aaagcgccgg gctcacctcg ctcgacacca tcccgcgaat
     1021 tcgcaaacac gtcgatgacg cgggcaatgt catcccctac tgggcgtggg cagataagcg
     1081 cgccaagcct ggccgcgcgc tgatggccga cgtgttcttc ctggtgttgc cgaagtagaa
     1141 agcttcacgg acacatcttt cttacgagcg ccacgcgcga ttccacgttc agcttgcgga
     1201 acacctggcg cacctggctc ttgatggtgt tcgcggacac gccgcgcaga tgagcaatct
     1261 gcgcgagcgt ccgtccttcg caaagcagct cggccactgt ttgttcggcg cgtgtcagcg
     1321 gtgaatcatc ggccggctgg tgcagcacgc aggtcatggc gaaagggacc gcggcctcgg
     1381 tcgcaccctc caggcgaaag gcgacgccat tggcgcgttc ttccaccgcc agtgttttgc
     1441 agttgccgag gaggcgcgca agcgcaggcg cggcgtcggg aacacgaatc tgccgacgca
     1501 tgcgtgccgg accgctggcg cgcagctcgt cgaggatgcg ggtcagtgac ttgggtgcag
     1561 gcatcgtggc gttctgccac gaaaacccga atcaccggca ctcatatatc tgcgcaatca
     1621 ccgcagatat gaatggggac ggtttgcttc aggtgagttt cttgacccag gcgacgtact
     1681 tgtccatcca gccatgcagg aacttgcgcg tctccgcgct cgcgaagttg ccggcgtcgt
     1741 cgaagaagcc gtctctgacc tgtatgtacg cctcgggctg ccccagcgtg ggcatgtcga
     1801 gatacgcaag aatggttcgc aggtgtagct gcgcgaccgc ggtgctgatg gcgccgacgg
     1861 aagcgccgat gacccccgcg ggtttgcccg cccaggcgct ctgcccatag gggcgcgacg
     1921 catgatcgat ggcgttcttg agcacgcccg gaatggaccg gttgtactcc gcggtcacga
     1981 agatgacggc gtccaccgcc cgcagctcgg tcttgagccg ctggacctcg ggcgcctgtg
     2041 ccttgtcgtc gtcctggttg tacaggggca ggtccccgat acgcagctct ttgaacgtga
     2101 agtcgggtgg cccgagcttg atgagcgcat ttgccagctt tcgattgatg gattccttgc
     2161 ggaggctccc gacgacataa ccgatctgct tgggcattca tgactcctgg tggcggtcga
     2221 tcatctcaaa tttgcccccc tattctgtag gaggacggcg cgccccgcgc catgcatccg
     2281 gagacggcag accttcgtat cgtcttccgc tatgcacaaa cgcggccttc agatcctgtt
     2341 caccttcatc ctgctatctc tgaccgccta caacctgtgg gtcagcgctc aacagccggt
     2401 ctgggagtgg ggtggtttga caacgtctcc tgacaatctc tggaccatcg cgacgctgat
     2461 cgacgcgtac tacggtttca tcacctttta tgtgtgggtc ctgtggaagg agacgcgggc
     2521 cctgcctcgc gtgtcctggt tcgtcgccat catgcttctc ggcaacatcg cgatgtcggt
     2581 ctatgtcctg cgccagctcg cgcggctcgg tccgtcggac tccatgggca ccctgctctc
     2641 cgctcgcaac gcgtgacccc atgcgcgact tcctgcaaaa gcggctggtc aaccccctgc
     2701 tggcgctatt gcggcaaggc gtgacaccac gggacctggc gatgagcgtg gccctcggca
     2761 cgagcctcgg gctggtgccg gtgctcgggg tatccaccgc cctgtgtgcg ctcgccgccc
     2821 tcctgctgcg cctcaacatg ccggcgatcc agatcgtcaa ctatctactg acgccgctgc
     2881 agctcgtcct gatcatcccc tggctgcgat ttggcgaaat gctggccggc gcgccgcgtt
     2941 ttccggtgac gctggattcc ggcctcgcgc tgctctcgca tggcgccatc aacgccatcc
     3001 aggtcctggg cctcgcgatc ctgcacgcca ccttcggctg gctggtgctc gcgccggtcc
     3061 tggccttcgt gctgtaccgc ggactcgaac ctgcttttcg ccatctgcga ccggaagcct
     3121 catgacctca cttgcgatgc aactgctgga acgcgatctc gttccggact tcctgattcg
     3181 ccggcgcatt cgcacactgc tcgccgcgcg cctgcgcgag gaggatcaac gcgacccggc
     3241 gaaacagcag cagcggctca tcgatttcat ccgcgaactc gaagtaagcc cggtagccat
     3301 cgagacggcc gctgccaacc agcagcacta cgaagtgccg accgagttct atacgcgtgt
     3361 gctcggcaaa cacctcaagt attccagttg cctgtatccg catccggacg ccgcgcccgc
     3421 cgagaacctc gacgccgccg aggcgcgcat gctcgggctc acctgccagc gcgcacggct
     3481 cgacaacggc cagcagatcc tcgagctcgg atgcggctgg ggatccctga gcctgtggat
     3541 ggccgctaac taccccgggt ccaggatcac cgcggtgtcg aattcccgaa cgcagagacg
     3601 ccatatcgag caccaggcgg ccgcccgcgg cctgacgaac ctcgaggtga tcacctgcga
     3661 cgtgaaccag ctttctttcc cggacgaccg gcgcttcgac cgcgtggtgt cggtcgagat
     3721 gttcgaacac atgcgcaatt acgacacgtt gctggcgcgg gtcgcgtcgt ggatggcgcc
     3781 gggcgccaca cttttcgtac acattttcac gcaccgcgag tacgcctatc cgttcgaggt
     3841 gcgcgatgaa agcgactgga tggcacgtca cttcttcacc ggcggcatca tgccgagcga
     3901 cgacctgctg ctgtatttcc agcgcgacgt gcgattgatc gagcattggc aggtgcccgg
     3961 ctggcattac cagctcacca gtgaagcctg gctttcgaac atggaccgga accgggctga
     4021 actcatgcca ttgctggcgc gaacctatgg cgagccccag gcgctgcgct ggtgggtgta
     4081 ctggcgcgtc ttcttcatgt cctgcgcgga gctgtgggga tacgccgacg ggcgcgagtg
     4141 gctggtgtcg cattacctgt ttgaaaaacg ctgaaaagcg gtagagtcgc aggccatggc
     4201 tgctgacaac tctttcagat ggctaaccgc cacggctctc tgcttcgcct cgtttctgca
     4261 ggcgcagccc gctttcgccg tcaccgatgc cgaccgcgtg gctgtctaca aggaattccg
     4321 gctgaactac gacgcgaaga aatacgtcga agcacagccg ctggcggaac gtctcgtggc
     4381 gctcaccgaa gagcaatacg gcgccgaaga actggcgttg accaacccgc tcaccaacct
     4441 cgccacggtg cactacaagc taggccacta tccggcggcg atcgaaaact accagcgcac
     4501 gctgcgcatc ctgcaggcca aatcgaccat cgccgacaag cagcagatcc gcccgctgca
     4561 cggcctcggc gtcagcttca tgggtgcgaa cgatcccgaa tccgccgtcg tggccctgaa
     4621 acgcgccgcc gacctgtcac gcaacaccga tggcctgttc aacatcaacc aggtggagtt
     4681 catcgacgcg ctgatcgacg cgtacgaagc cagcggccgt tatccggaag cggagaagga
     4741 agccctgtac gcgatgcgag tcgaggaagc ggcttatggc cgcgcgtcga tcaaactgct
     4801 cccccgcttc gacaaactcg cgcgctggta tgaaggcgcg cggcgctaca ccagcgaacg
     4861 caacatctac gaacgggcgc tctcggtact ggcgaagtcc gcgccggaaa acgacctgcg
     4921 gcgcgtgggc cccttgcgcg gcatcgcgcg ctccttccgg ctcgaagcct tctacggcgt
     4981 cgaaggcgcg gacagtggca gtaccttcaa ctccggcaac gcgggcgcgc cggtgttctc
     5041 cgatggaacg cagcaacgcc gcggcgaaag cgcgctgggc acggcgctcg acatcatcga
     5101 caggaatgcg ccggtcaacg aacagatgcg cggcgaggtg ctcaccgatc ttggcgactg
     5161 gtacctcatc gccaacgcgc tgcgccgcgc gtacgacacc tatgctgaag cctggaaggc
     5221 gtttgcgaag gcttccaata ccaagccgct cgagaggccg cgcgtgctgg cctatcggcc
     5281 ctcggtgagc tcgatcgatc gttcgcagct cgatccggcc gaggccgtca tcaaggtcgt
     5341 ggaattgcgt ttcacggtgg atcgcgacgg ccgcatcgac aacgtgactt caccgaccac
     5401 cgacgtgccc gagagcatcg tgcgcaattc gatgtcgtcg atgcgccgct cgcgttacgc
     5461 gccgcgcatc gagaatggcg cagccgttcc cacggccgac gtcgtcttcc acgagcgcgt
     5521 gctcgtgaag atcactgagt ccggatctga gacgtcatct gggaaggaga aagcggcgga
     5581 gaagccgccg gaggcggctc cggagccgtc gacaccacca ccggctggtc gttgatcggc
     5641 actgcctcga tcgtcatgtc ggcgatcgcc gccgccgctt ccggtgagcc gggctcggga
     5701 cgggacttgg gctgaagcgc ctgcgaagcc agcaagggtt tcgcgagatc cagtgtcggg
     5761 aactgcgtga ggaatttcgc cgccgcatca cgcatggccg cgtatgccgc gatccccagc
     5821 tcttcttctc cgttgccgat cttcggcgcg gggccactgc cgtatccggt gagcgtgagc
     5881 gtgtcgacca gctcgccgtt cggcgcgtag atgaagatct ggtagcggat ggtgaccgcg
     5941 gtatagacac cacccgtttc gcgggcgctg gcgaaagaaa actgttcgat gcgtggctcg
     6001 aagatggcca ggattccggg ctcgagtcgc gcggcatcga cggtctcgaa catcttcgcg
     6061 tcggtgaatt ccgcgcggaa gatttcttcg acgaggtgtt tgtgcgattc gccgagctgc
     6121 gcctcgtagt tcacgctggc gcgcgactcc ttgtgcacgt agttggcggc ttcggtggtg
     6181 accacgacgc cgaccttggc gggcgtaggc acgatgatgg ctttcggcaa gtcgccttcc
     6241 ggccgtactg cgacggcggt gcagccaccg agcagcagcg tgaacgcggc gaaaggaatg
     6301 gatagttgcg agatgcggtt tacgttcacg cgttcacccg tcagtgatgc agcgaggttg
     6361 accgaagacg tcgaccttga tcgcgcagct cgcaccaccg atggtgaggc ccacgaaggt
     6421 cccttcgttt tcgtcgcaca tggcgcgcat tagaacagaa aaccgctgat tgatacgcgg
     6481 cggcgagctg gccccggctg gcatgccgat ggcatggatg cggatcttgc ggcgcccctg
     6541 gcggtccatc gggttgtact tacgtactgc ctgcaatgcg gcttcgacgg acgggccggt
     6601 gaaatcgtcg ccgatcacgt acacgctgat cttcttgtcg gccgaccacc acccctggac
     6661 cgcggcgccg atgccctcga ccgggctcga atcgctgaac ggcttc
//
LOCUS       DATDEU010000105         3957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1916864, whole genome shotgun sequence.
ACCESSION   DATDEU010000105 DATDEU010000000
VERSION     DATDEU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916864"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VIV63_02535"
     CDS             <1..1095
                     /locus_tag="VIV63_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123502.1"
                     /translation="KVDDAGAPLSCVNYYRPDATTGELYSLPAFLAAYDPAGPWGRVN
                     PTGPQERARVASAAAQSARVRIRIPANTTIFGIGEKPTLRGVWFDIRPSTSSGNQPMN
                     VIIRNVHFEDAGVDCFPQWSPTDGSLGNWNSAYDAISVRNATHVWIDHNRFTDVDTAD
                     ETLPVLLGRIYQVHDGHVDVTNESDYVTVSWNQFLHHDKTMLIGSSDSATADRGKLRV
                     TIHHNWFGDVGQRVPRVRFGQVHVYNNYFEVPPGAAFGYSWGVGVESAIYAEKNFLEI
                     AAPFGAADVIDRFVGTRISDVSNCLLAAGGECVITDFVGAWNAAFNPDLVPDAGWTPT
                     LYGKNGSADKARDVRQFVLDGSGPDDSRSR"
     gene            1477..2703
                     /locus_tag="VIV63_02540"
     CDS             1477..2703
                     /locus_tag="VIV63_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123503.1"
                     /translation="MGTTGGSAAQAARTVTVTNRKELVAALAYPDPSSKLIYIKGVID
                     VNVDDAGRVLTCADYAQPDPATGEMFSIHAFMAMYDPAGPYGRENPFGGQEEARKASA
                     AAQAARVRIRIPPNTTLYGVGSDATLIGAWLDIRGSGGSQPMNVIVRNISFQDTADCF
                     PEWSPTDGVTGNWNASYDSISIAHATHVWIDHNRFADLRTRDDTQPVYFGHRQQLHDG
                     HLDITDESDFVTVSWNQFTAHDKVMLIGNSDSSTADREHLRVTLHHNLFDGTGQRTPR
                     VRFGKVHVYNNVYRADRNTNYRSSWGAGTESQIYAENNYFEMSASFGPMEVIDGKKGL
                     GMTVVGNCWRSKEGACAPTDFLALYNAQFDPDLKPDAGWTPSLYGSAKSAEPAESARE
                     RVLTESGPGRAYNNKN"
     gene            complement(2771..3133)
                     /locus_tag="VIV63_02545"
     CDS             complement(2771..3133)
                     /locus_tag="VIV63_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEY6123504.1"
                     /translation="MTTPNPHGLTQLRDALRQFAADRDWDQYHSPKNLASALAVEAAE
                     LLERFQWLTEDQSRALPPAELQKVREEMADVLNYLVRLADKLDVNLIEAAREKMKLNE
                     QKYPADKARGSAKKYSEL"
     gene            3208..>3957
                     /locus_tag="VIV63_02550"
     CDS             3208..>3957
                     /locus_tag="VIV63_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY6123505.1"
                     /translation="MRIPFPLSVAALIAVLSLPARAADVWDAPAFSVPARELLQAATA
                     VKRERATAVVVLLDERNFVLDEQHRLTTISRLIYRIDSPDGVERWAASTAHWQPWHQA
                     KPTIRARVVTLDGREHLIDQKLLTDAANRSGDNQVYDDSHTLEGPLPAVAIGAVIEEE
                     ITVRDEKPFFPAGSVFREFIGRPMPVLRTRVSIDAPESLPLKHTTQLLPRAVVQEIRA
                     NGRVTWTLEQGLIDEMSEMEPNLPPDVPSWPM"
BASE COUNT          743 a         1291 c         1246 g          677 t
ORIGIN      
        1 aaggtcgatg acgcgggcgc gccgctgagc tgcgtgaatt actaccgacc cgatgcaacg
       61 accggagagc tctattcgct gcccgccttt ctcgcggcct acgatccggc cggtccctgg
      121 ggccgcgtca accccaccgg tccgcaggaa cgcgcgcggg tcgcctcggc ggcggcgcaa
      181 tcggcgcgcg tgcgcatccg gatcccggcc aacaccacga tcttcggcat cggcgaaaag
      241 ccgacgctgc gcggcgtgtg gttcgacatc cggccaagca cgagcagcgg caaccagccg
      301 atgaacgtga tcatccgcaa cgtgcatttc gaggacgcgg gcgtggactg cttcccgcag
      361 tggtcgccca ccgacggctc gctgggcaac tggaactcgg cgtacgacgc catctcggtg
      421 cgcaacgcca cgcacgtgtg gatcgaccac aatcgattca cggacgtgga taccgccgac
      481 gaaacgctgc cggtcctgtt aggccgtatt tatcaggtcc acgacgggca cgtggatgtc
      541 accaatgaat cggactacgt gacggtgtcg tggaaccagt tcctgcatca cgacaagacg
      601 atgctgatcg gcagttccga cagcgcgacg gcggatcgcg gcaagctgcg cgtgacgatc
      661 catcacaact ggttcggcga cgtgggccag cgcgtgccgc gtgtgcgctt cggccaggtg
      721 cacgtctaca acaactactt cgaagtgccg ccgggcgcgg cctttggcta tagctggggc
      781 gtcggcgtgg aatcggcgat ttacgcggaa aagaatttcc tcgagatcgc ggcgccgttc
      841 ggagccgcgg atgtcatcga ccggttcgtc ggcacgcgca tttcagacgt gagcaattgc
      901 ctgctggcgg cgggcggaga atgcgtgatc accgacttcg tcggcgcgtg gaacgcggca
      961 ttcaatccgg atctcgtgcc ggacgccggc tggacgccga cgctgtacgg aaagaatggc
     1021 agtgccgaca aggcgcgcga cgtgcggcag ttcgtgctcg acggcagcgg gccggacgat
     1081 agccgttcgc ggtagtagat agggtcaggc accgattcac aggaaacggt gcctgacccc
     1141 attttgttaa agatgcattg cgtcatcggg ggcgaatctg tgaccacact cgcagccgcg
     1201 cgatgaatcc gaacaaatcc ttgcgtccgc ctgaaatccc ttacaggatg tcaccggtgt
     1261 cattattgcg gctggtctga aagggccgca gttttatggc ggttttaacg agggaaatcg
     1321 atgtttttac cttgcctggc ccgggcgctt gcgtacttgc tgtccatcgg tttcctacta
     1381 ggaaccgcta aggcggctac ccctcctgcc gacggtggac gcgagatatt gggcataaac
     1441 gacggatggg gggccgttcc cacggcgtcc ctccccatgg gcaccacggg tggctcggcg
     1501 gcccaggcgg cgcgcacggt cacggtgacg aaccggaaag agctcgtcgc cgccctcgcc
     1561 tacccggacc cctcgtcgaa gctcatctac atcaaaggcg tcatcgacgt gaacgtggac
     1621 gatgcgggcc gcgtgctcac ctgcgccgat tacgcccagc cggatccggc gaccggcgag
     1681 atgttttcca ttcacgcgtt catggcgatg tacgaccccg cgggtcccta tggccgcgaa
     1741 aacccgttcg gcgggcagga agaagcgcgc aaggcttccg cggccgcgca ggcagcgcgc
     1801 gtgcgcatcc gcattccgcc gaacaccacg ctttatggtg tcggcagcga cgccacgctc
     1861 atcggcgcgt ggctagacat ccgcggcagc gggggcagcc agcccatgaa cgtgatcgtg
     1921 cgcaacatca gcttccagga cacggcggat tgttttcccg agtggtcgcc caccgatggt
     1981 gtcaccggca actggaacgc ctcgtacgac tccatctcga tcgcccatgc cacgcacgtg
     2041 tggatcgatc acaaccgttt cgctgatttg aggacgcgcg atgacacaca accggtctat
     2101 ttcggccacc gccagcagct gcacgacggt cacctcgata tcacagacga atcggacttc
     2161 gtcactgtgt cgtggaacca attcacggca cacgacaagg tcatgctcat cggtaattcc
     2221 gatagctcga cagcggaccg cgaacacctg cgcgtaaccc tgcaccacaa cctgttcgac
     2281 ggcaccggcc agcgcacacc acgcgtacgg ttcggcaagg tgcacgtcta caacaacgtc
     2341 taccgcgccg accgcaacac taactaccgt tcgagctggg gcgcgggcac cgagtcgcag
     2401 atctacgccg agaacaacta cttcgagatg agcgccagct tcggccccat ggaagtcatc
     2461 gacggcaaga agggcctcgg gatgacggtg gtgggcaact gctggcgcag caaggaaggg
     2521 gcatgcgcgc cgacggactt cctggcgctc tacaacgccc agttcgatcc ggacctgaag
     2581 ccggatgccg ggtggacacc cagtctttat ggttcggcca aatcggctga accggcggaa
     2641 tcagcacgag aacgggtgct gactgaaagc ggcccaggac gggcctacaa caacaaaaac
     2701 tgacagacgt cagggagatg ggcatggggc gaggcgcagc gactaaactg gcgcctcgct
     2761 ccatagctct tcagagctcc gagtacttct tcgcgctgcc ccgcgccttg tccgccgggt
     2821 acttctgctc gttgagcttc atcttttccc gcgccgcctc gatgaggttc acgtccagct
     2881 tgtcggccag gcgcaccagg taattcagca cgtcggccat ttcctcgcgc accttctgga
     2941 gttcggccgg cggaagcgcc cgcgactggt cttcggtcag ccactggaag cgctcgagca
     3001 actcggccgc ctcgacggcc agcgccgacg ccaggttctt ggggctgtgg tactggtccc
     3061 agtcgcggtc ggcggcaaat tgccgcaagg cgtcgcgaag ctgggtcagg ccgtgcggat
     3121 tcggtgtagt catggccggc gagactacat caaggcgtga acaattcacg ctttgggcgc
     3181 ctttcctggg gatatgcttt tgggcgtatg cgcataccgt tcccactgtc cgtggcggcg
     3241 ctcatcgccg tcctgtccct gcccgcccgc gccgcggacg tctgggacgc gcccgcgttc
     3301 tcggtcccgg cgcgcgaact tctgcaggcg gccaccgccg tcaaacgcga acgcgccacc
     3361 gccgtggtgg tgttgctcga cgaacgcaac ttcgtgctcg atgagcagca tcggctcacg
     3421 accatctcgc gcctcatcta tcgcatcgac tcgcccgacg gcgtggagcg ctgggccgcg
     3481 agcaccgcgc actggcagcc ctggcaccag gcgaagccca cgattcgcgc gcgcgtcgtc
     3541 acgctcgacg ggcgcgaaca cctcatcgac cagaagctgc tgacggacgc cgcgaaccgc
     3601 agcggagaca accaggtcta cgacgactcg cacacgctcg aagggccact gcccgcggtc
     3661 gcgatcggcg cggtcatcga agaagagatc acagtgcgcg acgagaagcc gttcttcccg
     3721 gcgggctcgg tgttccgcga attcatcggc aggccgatgc cggtgctgcg cacgcgcgtg
     3781 agcatcgacg caccggagtc gttgccgctc aaacacacca cgcagttgct gccacgtgcg
     3841 gtggttcagg aaatacgcgc caacggccgc gtcacctgga cgctcgagca gggcctcatc
     3901 gatgaaatga gcgagatgga accgaacctg ccgccagacg tgccgtcgtg gccgatg
//
LOCUS       DATDEU010000106         3274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1333849, whole genome shotgun sequence.
ACCESSION   DATDEU010000106 DATDEU010000000
VERSION     DATDEU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333849"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /gene="ccoS"
                     /locus_tag="VIV63_02555"
     CDS             complement(<1..142)
                     /gene="ccoS"
                     /locus_tag="VIV63_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY6123506.1"
                     /translation="MSISYLLVALGLTAFGGTVWALFWAVDSGQYDDLEAEGAAILDD
                     ADA"
     gene            complement(139..2358)
                     /locus_tag="VIV63_02560"
     CDS             complement(139..2358)
                     /locus_tag="VIV63_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY6123507.1"
                     /translation="MSAAETLHARWSLDDGQHEHVVFLVEGMRCAGCARNIEKVVRAL
                     PDIENVSVNAANARVSVDWRGRGVTTLPRILSAVDGAGFRPVPLAGNDASQRFREERR
                     IALKRLGLASIGMMQSMMYLAALYGVSDIDAAMAQLMRVAGMVIVTPVLFYSGAPILQ
                     GAWRDLSQRRVGMDVPVALALLLAWLPSVMNTFRGTGEVYFDSVGMFVFFLTAGRFLE
                     MKARHRGATSAEALARSLPAQVTRLLPDGGREQISASALIAGDRFLVPKGDVIAVDAR
                     LPPDVPADFVVLIDESLLTGESCAVRRGVADTIRGGSVNIGNPLTMVAVAAVGQSTLA
                     AIVALQERARTERPRLVRLADRAASWFVAVILCLALATGVIWWVVAPERAFSAVLAVL
                     VVTCPCALSLAMPMALAAANTRLARLGVLVTRADAIERLARIDTVIFDKTGTLTLPVT
                     GVSDVKLLSGMDRERVLAIAAALERDSAHPLATAFRSHERADLRASAIREVAGAGIEG
                     SIGARRWRLGKRDFVEGSAFNGKVVPLRQPGTEDATLYLGSEDGLAAALRVGAPLRPE
                     AARALAALRSQGLGTIMASGDSAGAVQEAARALGIRQFHSRLTPSEKIWLLHELRSHG
                     HRAFMIGDGINDGPVLAAAEVSCAMGQGSAIAHSAADLLLVNENLEVLPQTVRVARNA
                     MRVVRQNLTWSLVYNFSAVPAAALGFISPWLAALGMSLSSLAVVMNARRLASQRISA"
     gene            complement(2355..2846)
                     /locus_tag="VIV63_02565"
     CDS             complement(2355..2846)
                     /locus_tag="VIV63_02565"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEY6123508.1"
                     /translation="MKKNPVFWLMWLLPGAAVVASFVTLAIAVQGADRALPALYHWEG
                     ERLDADFERARNAARHNMRATLAFAGGAEPCTAMVIPAPGDPASITVNFTSTSDASLD
                     RTLTMQREKAGVYRVACEVLPRGRWFVSLQDAAGAWVLRGSLNDKAQVAVLQARDPAG
                     DGA"
     gene            complement(2849..>3274)
                     /locus_tag="VIV63_02570"
     CDS             complement(2849..>3274)
                     /locus_tag="VIV63_02570"
                     /inference="COORDINATES: protein motif:HMM:NF023045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HEY6123509.1"
                     /translation="TRILRPRTLIYGSILGLLMVGFVVAVAMRSPVSLDVIRDRNALF
                     RLTDDGNVDNVYTIRILNKSEHPQRLVLEARGASPLTLIAKQTEYRVPSGAVYSLPLR
                     VRRAAYEPLGAETIVFTLRSLDDPSVQTETEARFLAPTR"
BASE COUNT          606 a         1152 c         1032 g          484 t
ORIGIN      
        1 ccgcatccgc atcgtcgagg atggccgcgc cctccgcctc gaggtcgtcg tactggcccg
       61 aatcgacggc ccagaacagc gcccagacgg taccgccgaa cgcggtgagt cccagcgcca
      121 ccagtagata ggagatgctc atgcgctgat cctctgcgac gccagccgcc gcgcattcat
      181 gacgaccgcc agcgaggaga gcgacatccc gagcgccgcg agccacggcg agatgaaccc
      241 gagcgccgct gccggaacgg cgctgaagtt gtagaccagg gaccatgtga ggttctgacg
      301 caccacgcgc atggcgttgc gcgccacacg cacggtctgc ggcaacactt cgagattttc
      361 gttcacgagc agcaggtcag ccgccgaatg cgcgatggcc gagccctgcc ccattgcgca
      421 ggaaacctcc gcggccgcga gaaccgggcc gtcgttgatg ccgtcgccaa tcatgaaagc
      481 ccgatggcca tgcgatcgaa gttcgtgcag cagccagatc ttttcactcg gcgtaagccg
      541 cgagtgaaat tgcctgatgc cgagcgcccg tgccgcctcc tgcaccgccc cggcgctatc
      601 accgctggcc atgatcgttc ccagcccctg ggagcgaagc gcggcaagtg cgcgcgcggc
      661 ctccggacgc agcggagccc caacacgcag cgccgccgcc agaccgtctt cggatcccag
      721 gtacaacgtc gcgtcttccg ttcccggctg cctcagcggg accactttgc cgttgaacgc
      781 ggagccttcg acgaagtcac gttttcccag ccgccaccgg cgagcgccaa ttgagccctc
      841 gatgccggca cctgccactt cacggattgc cgaggcgcgc aggtcagcgc gctcgtggct
      901 gcgaaacgcc gtggccagag gatgagccga atcgcgctcc agggcggcgg caatggccaa
      961 caccctttcg cgatccatgc cggacagtag tttgacgtcg gagacgccgg tcacgggcaa
     1021 tgtgagcgtg ccggtcttgt cgaagatcac ggtgtcgatg cgtgccaggc gctcgatggc
     1081 gtccgcgcgc gtcaccaaca ctcccaaccg cgccaggcgc gtgttggcgg cggccagcgc
     1141 catcggcatg gcaagcgaca gcgcgcaggg acacgtgacc acgagcaccg cgaggaccgc
     1201 ggagaacgcc cgctccggag ccacgaccca ccagatcacg ccagtagcca gggcgagaca
     1261 caggatcacg gcgacgaacc acgacgccgc gcggtcagcg agccgcacga ggcgcggccg
     1321 ttcagttcgt gcgcgctcct gcaaggcgac gatcgcggca agggtggatt ggccgaccgc
     1381 cgcgaccgcc accattgtca ggggattgcc gatattcacg ctgccgccac ggatggtatc
     1441 ggcgaccccg cggcgcaccg cgcacgattc accggtcagc agtgattcat cgatcaacac
     1501 cacgaagtcg gcagggacgt cgggtggaag gcgcgcgtcc accgcgatca cgtccccctt
     1561 gggtacgagg aaacggtccc cggcgatgag cgcagatgcg ctgatctgct cgcgaccgcc
     1621 gtcaggcaac aaccgcgtga cttgcgcagg cagactgcgc gcgagcgcct cggccgacgt
     1681 ggctccgcgg tggcgtgcct tcatttcgag aaagcggcct gccgtgagaa agaatacgaa
     1741 catccccacc gagtcgaagt acacctcgcc agtgccgcgg aatgtattca tcacgctcgg
     1801 aagccacgcc agcaacagcg ccagcgccac gggcacatcc atgccgacac gccgttgcga
     1861 cagatcgcgc cacgcgccct gcaggatcgg cgcgccgctg tagaacaaca ccggggtcac
     1921 gatcaccatg ccggccacgc gcatgagttg cgccatggcc gcgtcgatgt ccgaaacacc
     1981 gtacaacgcg gccaggtaca tcatcgactg catcatgccg atcgaggcga ggcccagtcg
     2041 cttgagcgcg atccgtcgct cctcacggaa tcgctggctc gcgtcattgc cagcgagcgg
     2101 caccgggcga aaacccgcgc catccaccgc agacaggatg cgcggcagcg tggtcacgcc
     2161 gcggccgcgc cagtccaccg atacgcgggc gttcgcggca ttgactgaaa cgttttcgat
     2221 gtccggaagc gctcgcacca ccttttcgat gttgcgtgcg cagccggcgc agcgcattcc
     2281 ctcgaccagg aacacgacgt gttcgtgctg cccgtcatcg agcgaccagc gcgcgtgaag
     2341 cgtctcggcc gcgctcacgc gccgtcgcct gccggatcgc gcgcctggag cacggcgact
     2401 tgcgccttgt cgttcaggct gccacgcagc acccatgcac ctgcggcgtc ctgcaggctg
     2461 acgaaccagc gtccgcgcgg caacacttcg catgcaacgc gatacacgcc agccttttcg
     2521 cgttgcatcg tcaacgtccg atccaggctc gcatcgctgg tgctggtgaa attcacggtg
     2581 atcgaagcgg gatcgccggg agccggtatc accatcgccg tgcacggctc cgccccgccg
     2641 gcaaaggcca gtgtggcgcg catgttgtgg cgcgccgcgt tgcgcgcgcg ttcgaagtcc
     2701 gcatcgagcc gctcgccttc ccagtgatag agagcgggta acgcccggtc agcgccctgc
     2761 accgcgatgg caagcgtgac gaaactcgcg accaccgccg caccgggcaa taaccacatc
     2821 agccagaaaa ccggattctt cttcacgatc acctcgttgg cgcgagaaat cgcgcctcgg
     2881 tttcggtttg cacggacggg tcatcgagcg atcgcagcgt gaagacaatg gtctccgcgc
     2941 ccagcggctc gtatgccgcg cgccgcacgc gcagcggcaa cgagtacacg gccccggacg
     3001 gcacgcggta ttcggtctgc ttcgcgatca gcgtcagggg tgaagcaccg cgcgcctcga
     3061 gcaccagacg ctgcgggtgc tcgctcttgt tgaggatgcg gatggtgtag acgttgtcga
     3121 cgttcccgtc gtcggtgagc ctgaacagcg cgttgcggtc ccggatcacg tccagcgaca
     3181 ccggcgagcg cattgccacc gccaccacga accccaccat gagcagcccc aggatcgacc
     3241 catatataag agtgcgcggc cgcaggatcc tcgt
//
LOCUS       DATDEU010000107         3505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_20625, whole genome shotgun sequence.
ACCESSION   DATDEU010000107 DATDEU010000000
VERSION     DATDEU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20625"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3292
                     /locus_tag="VIV63_02575"
     CDS             <1..3292
                     /locus_tag="VIV63_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEY6123510.1"
                     /translation="AALCIAVGSTVQALAGRALVARMIGVPLHLRSARDALVVLLLAG
                     PVTCVIAATIGVGTLLALGIIGNDAIVGNWLAWWSGDSLGVLVFTPLVLLAPGGRAQV
                     SWRGEPIGQLPFAALLLLLLPLGLTFYAWQVISENDYQRGDAKFETLAIESEKALENR
                     LASYGNVLLGAASFIQGSADVTRMEWRAYAESIRMRDNFPGLNGIAWVEPVETDRIAD
                     FVRRRRTDGAPDFNVHPQVAGIPNYVVTFVEPESENLPAIGLNIAFERNRLEAANLAR
                     DSGRAAITGLVLLAQNERRTPAFLMLYPIYHRNMPTNTTAERRAALRGWTNGPLVARN
                     FLASLTDRQGVDYRLRIYDGNTELPEALIYASAGASTTQSAFVKRGAVQIMQRQWLLV
                     WESTPAFDLAQRSTNPLFVLIGGLLFTGLLSLLLVVLTVRRTEHIEHLVGERRFIVPS
                     LVFLLLAVGSFALYKRLLDQELAFVQRQLNSDLEEIASQLRARTAERITPLTRMAARW
                     KSAGGTDYELWRSDAANLTSELPGLRALQWIDASYHVRWIVPLTGNEGELDRDVLAGN
                     DGAAALRGAGASGHEILTSPGSMPQGYVGFSAYLPVQRDGRFDGFIAGQFSIEHLFNG
                     SVDARLSTDYAISILREGNTIFANSRGESPLMRELATERPLRVGSQEWTLRMVPTPDF
                     VERQKSGLPMLVLGAGLLVAALAALSVRYMLISRLKSAHLAKSLALNAGIISSSAHLV
                     IAIDEQYRIMIFNRAAEKALGYSAPEVVGKRAIPIFMDPSELLERARSLSAELGESVP
                     LGPEILTRIPLRDGLETRAWTFVRKDGTRFPTNVIITPLRDEHGATAGFLGVIEDVSA
                     RHEVDRMKSEFTAVVSHELRTPLTSIRGSLGLILGAFSQSLPQKVRDLLEIAQNNSDR
                     LVLLINDILDIEKFSAGQMRFDIQAVSLGDIVRQAVESTDGYARKHDVNVELQPVDPA
                     HRVDVDPDRFVQVLTNLLSNAVKYSPARATVHVTAERRGDTLRVSVRDSGQGIPEAFR
                     KRIFERFSQADSSTTREKGGTGLGLYIARRFVEQMFGRIGFDSEPGKGATFWVELPAS
                     ASAAARTA"
BASE COUNT          586 a         1185 c         1108 g          626 t
ORIGIN      
        1 cgccgcgctg tgcatcgcgg tcggatcgac cgttcaggcg ctggccggcc gtgctctcgt
       61 cgcgcggatg atcggcgtgc cgctgcacct gcgctcggcg cgggacgcgc tggtggtgct
      121 gctgctcgcc ggtcccgtca cctgtgtcat cgccgcgacc atcggggtcg ggaccctgct
      181 ggccctgggt atcatcggca acgacgcgat cgttggtaac tggcttgcct ggtggtcggg
      241 cgactcgctg ggcgtactgg tattcacccc gctggtgttg ctcgcgcccg gcggccgggc
      301 ccaggtgtcg tggcgcggcg aacccatcgg ccagcttccg ttcgcggcac tgctcctgtt
      361 gctgctgccg ctcgggctca cgttctacgc gtggcaggtc atctccgaaa acgactacca
      421 gcgtggtgac gccaagttcg agacgctggc catcgagagc gaaaaagcgc tggagaatcg
      481 gctggcctcc tatgggaacg tattgctcgg cgccgcgagt ttcatccagg gctccgccga
      541 cgtaacgcgc atggaatggc gcgcctatgc ggaatccatt cgcatgcgcg acaacttccc
      601 ggggctgaac ggaatcgcct gggtcgagcc ggtcgaaacg gatcgcattg cggatttcgt
      661 gcgcaggcgg cgtacggacg gcgcccctga cttcaacgtt cacccccagg tggccggcat
      721 tcccaactac gtcgtcacgt tcgtggagcc ggaaagcgag aatctcccgg cgatcggcct
      781 gaacatcgcg ttcgagcgca accggctgga agccgccaac ctggcccgcg attcgggccg
      841 cgccgcgatc accggcctgg tgctgctggc gcagaacgaa cgccgcacgc cggctttcct
      901 catgctctat cccatctacc accgcaacat gccgaccaac acgacggctg aacgtcgcgc
      961 cgcgcttcgt ggctggacca acggacccct ggtggcgcgt aattttctcg cctcgctcac
     1021 cgaccgccag ggtgtcgact atcgcctgcg catctacgac ggcaacaccg aacttcccga
     1081 agcgctgatc tacgccagcg ccggcgcgtc caccacgcaa tcggccttcg tcaagcgcgg
     1141 cgcggttcag atcatgcagc gccagtggct gctcgtctgg gagagcacgc cggcattcga
     1201 tctggcgcag cgttcgacca atccgctgtt cgtcctgatc ggcggcctgc tgttcaccgg
     1261 acttctgtcg ttgctgctcg tggtgttgac cgtccggcgg accgaacaca tcgaacacct
     1321 ggtgggcgaa cgccgcttca tcgtaccgtc gctcgtattc ctgttgctcg cggtgggcag
     1381 tttcgcactg tacaagcggc tgctcgacca ggaactcgcc ttcgtgcaac gccagctcaa
     1441 tagcgacctc gaggaaatcg cttcgcagct acgcgcgcgc accgccgagc gcatcacgcc
     1501 gctgacgcgc atggcggcgc gctggaaatc cgcgggcggc acggactacg aattgtggcg
     1561 cagcgacgcg gccaacctca ccagtgaact gcccggcctg cgcgcgctgc agtggatcga
     1621 tgcgagctac cacgtccgct ggatcgtgcc gctgaccggc aacgaaggcg aactggatcg
     1681 cgacgtactc gcggggaacg acggcgccgc agcgctccgc ggcgccggcg cgtccgggca
     1741 cgagatcctg acctcgcccg gctccatgcc acaggggtac gtcggtttct ccgcctatct
     1801 accagtgcag cgcgacggcc gtttcgacgg attcatcgcc gggcagttct cgatcgagca
     1861 cctcttcaat ggatcggtcg acgcacgtct gtcgaccgac tacgcgattt cgatcctgcg
     1921 tgaggggaat acgatcttcg ccaacagccg gggcgagagc ccgctgatgc gcgagctcgc
     1981 gaccgaaagg ccgcttcgcg taggctcgca ggagtggacg ctgcgcatgg tgccgactcc
     2041 agacttcgtg gaacggcaga agtccggctt gcccatgctg gtgctcggcg cgggtctgct
     2101 ggtggccgcg ctcgcagcgc tctcggtccg ttacatgctg atctcacgcc tgaagtccgc
     2161 gcacctggcc aaatcgctgg cgctcaacgc cggaatcatt tcgagcagcg cgcacctggt
     2221 catcgccatc gatgagcagt accgcatcat gatcttcaac cgggccgccg aaaaagcgct
     2281 gggctactcc gcccccgagg tggtcggcaa acgcgccatc cccatcttca tggacccgtc
     2341 ggaactgctg gagcgcgccc ggtcgctctc ggccgaactc ggcgaaagtg ttccgctggg
     2401 tcccgagatc ctgacgcgca tcccgctgcg cgacggcctc gaaacgcgcg cctggacttt
     2461 cgtgcgcaag gacggcacgc ggttcccgac caacgtgatc atcacgccgc tgcgcgacga
     2521 acacggcgcc acggccggat tcctcggcgt catcgaggat gtcagcgcgc gccacgaagt
     2581 cgatcgcatg aagagcgagt tcacggcggt ggttagccac gagctgcgca cgccgctcac
     2641 ctcgatccgc ggttcgctgg gactcatcct cggtgcgttc tcgcaaagcc tgccgcagaa
     2701 ggtccgtgac ctgctcgaga tcgcgcaaaa caactccgat cgactggtgc tgctgatcaa
     2761 cgatattctc gatatcgaga agttcagcgc gggccagatg cgcttcgata tccaggccgt
     2821 gtcgctcggc gacatcgtgc gacaggcggt cgagtcgacc gacggctacg cgcgcaagca
     2881 cgatgtcaac gtggaactcc agcctgtcga tcctgcgcat cgggtagacg tcgatccgga
     2941 ccgcttcgtg caggtgctga ccaatctgct gtcgaacgcg gtgaagtatt cgcccgcccg
     3001 cgccaccgtt cacgttacgg cggagcgccg cggcgacacc ctgcgcgtga gtgtgcgcga
     3061 cagcggccag ggaattccgg aagcctttcg caaacggatc ttcgaaagat tctcgcaggc
     3121 cgactcatcg acgactcgcg aaaaaggcgg aaccggactc ggtctatata tcgcacggcg
     3181 tttcgtcgaa cagatgttcg gccgtatcgg cttcgactcc gagcctggca agggtgcgac
     3241 gttctgggtg gagttgccgg catcggcatc ggcggcggcg cgcaccgcct aacgactgcc
     3301 gataagggaa aacacgcaga cggtcgcgcg gcgcttccac gcccctgcgg ctgcgattac
     3361 tctgcggcag tgcaggacgc agcagcatcg aacaattcga gcccctggcc gcggtggcgc
     3421 gcatacgtgt tcggctttgt cttctcggcc gccatcctgc tgatgcgagt cctgatcgag
     3481 ccgtggatgg gccatcaacc cttcc
//
LOCUS       DATDEU010000108        10029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1333959, whole genome shotgun sequence.
ACCESSION   DATDEU010000108 DATDEU010000000
VERSION     DATDEU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333959"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VIV63_02580"
     CDS             complement(<1..988)
                     /locus_tag="VIV63_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6123511.1"
                     /translation="MSILWIIAAANGGVALPAPTPEVVVTGSRVATTPDKISANVTVL
                     EREDFDVEKPAKLADVLRRVAGVHVDQVGGRGGTGSVYVRGADPNYTLVLIDGVRVND
                     PTNARGGSFDLSALDIADVERVEVARGPYSAIYGGDALAGVINIVTRHVPAAKSYASL
                     DAMGGAYDTREIAVTAGGPAVGGDWNMGFSDTNEGEQVRGNHFESQRVSGNFNTELGA
                     FTTLSVSGRYSESQREGFPDDSGGYEFAAIRDVEKRDADESVFGFGVSHRAGDATFAL
                     ALGYFDRDDHIDSPGVAPGIRDPFGVPPSIVDSTLTRFTATFTGTQKFSDNLT"
     gene            1061..4255
                     /locus_tag="VIV63_02585"
     CDS             1061..4255
                     /locus_tag="VIV63_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123512.1"
                     /translation="MSRFLCRYAAGVLTIAALASFHIAEAQNAVPPEAAFRENPAWTV
                     EDGALTTKVAAPDRGLVTRVTLSDSISSFEYKAPVGSRAHFVVMGRYAFELVGNGNWQ
                     SFALRFRGPRFDSGFTKLDNAFALDVRNGAETRRSVFFEGPSAVAIWQGEDKRGPSFI
                     VAQGPFAVRNARHEAADFDQLTLPKAAGGDTNEKDLRDLVALGKQTFEDVGCHACHLV
                     DPSDTAVSSGPNLFGLIRGEPRTREVVEGGEGHRFQIKAGREYVRNSVRMPSEQLAVA
                     ESGPTKGQAYLPVMPPFTKDVLGDAQIDAISDYLATLNLPQDRGPVVKLAGLTPEPPY
                     DPMKDSLQWLVADTVRLQRGPLAGVSGRSIHVGNPNGVNYTFDPRLLAIVKIWQGGFL
                     DMTGELTNRGNRGLAIGYESREISFGERAYLVAPLNAAGAPVDFTFKEGKFGDTATFK
                     SWLYSKEDQLALIAKADAQFLGYTRDSKNKTAAPSFKYRVGKNTVDVTTSISERGEAA
                     IRVAGKLATEQSFVLNPALLTGVTVSGGSLSGDRWTLPAGSANATLSGKIAVAPAAWR
                     PAASNYAYERKPLERTPATATLPAGYSIENYYSPKDNYGRDQLFEALGLAVAKDGTVV
                     VATRTAGIWRLVKGEWRLFAEGLFDSLGVVIEDGKGLVVVAGQKAELTRISDTNGDGI
                     ADKYETLFDAHSYHGNYHSYMHGPVKGGDGAYYFALNLVHDGSGAAYTAGGNVMGTWG
                     GFNGWAVRVEPDGKFELFSKGLRSPASLGVGPDGRVWYADNQGDFVATSKMFVLGKDK
                     FYGHPAGLVDLPGMTPDSPDIAYDKWADRRERPIIMFPHNRVANSPGNPAWDTTRGKF
                     GPFAGQMLIGDQTQSNLLRVVVQKVGDVEEGSVMPFMEGLESGVMRPVFLPDGSLLLG
                     QTGRGWQAKGGKVAALQHVRWDGKTVAPQIKAMVATPDGFRVDLTQPLGNGVSEAILK
                     SALTVESWVYRDAPDYGSPELDLHGEDIASFTVSADRKSVAVKLLSIEQKTVHPRQTS
                     RIYHLKLASATLFDANAPAALNAYYTLSRFPAAK"
     gene            complement(4328..4945)
                     /gene="gstA"
                     /locus_tag="VIV63_02590"
     CDS             complement(4328..4945)
                     /gene="gstA"
                     /locus_tag="VIV63_02590"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEY6123513.1"
                     /translation="MQLYFSPGACSLASHITAREAGLPLDLTRADTKTKKLEDGSDYF
                     AINSKGAVPALKLDNGQVLTEGVAIMQYLADQKPETNLAPKNGTLERYRVQEWLNYIT
                     SEVHKSFSPLWNPANEQVVKDYALTNLKKKFDWLNVQLAGKKYLTGDTFTIADAYLFT
                     VVNWANPLGIDLTPWPALKEFQARVAARPHVQEAMAAEGLTKKAA"
     gene            5103..5465
                     /locus_tag="VIV63_02595"
     CDS             5103..5465
                     /locus_tag="VIV63_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6123514.1"
                     /translation="MPKAHGSANVFSSRCPSRRVMAVLAEKWTLLIVAQLANGPMRTA
                     EIRRGVDGVSEKMLIQTLRKLEAFGLVSRKSYPEVPPRVDYRLTPLGRSLSRLAGLFS
                     RWVERNVSNLLKAEQDAA"
     gene            5692..6177
                     /locus_tag="VIV63_02600"
     CDS             5692..6177
                     /locus_tag="VIV63_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123515.1"
                     /translation="MAVNAATAAEKVATEELAVLQAMAESINREASRPFERLYFESEF
                     EGAPHVASSLANPDRTKFCGLSREEAIELVSTLQALSTDPVEIDDEVAKSAGLKIGHK
                     KNPRFPYVILSRVVFGPGNKSAWVAVELNGSSGAVMRLDKVGGAWSKTARCGGWLKPE
                     D"
     gene            complement(6183..7298)
                     /locus_tag="VIV63_02605"
     CDS             complement(6183..7298)
                     /locus_tag="VIV63_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEY6123516.1"
                     /translation="MALLAACETSRKVATPGPATARFEPVSWSKLPGWRADDPLAAWP
                     AIVASCSVIHSRPAWQSFCGSVVAASPGDSEFVRGFLEQNLQPYHVLRVTDGKREKTG
                     LITGYYEPLLMGSRERSEQFATPLYRRPDDLLIVDLAEVIPELKGKRVRGRVVGHKVV
                     PYYSRAATREAPGLAGHEIVWIDNALDAFMLEVQGSGRVQLTTGETIRLQYEDQNGHP
                     YRSIGKYLADQGVMTIDQVNMSAIRAWLAANPQRLHEVLDTNPSVVFFREAPLDDASV
                     GPKGALAVPLTAGRSIAVDPKFQPLGAPMFISTTAPGSGLPLQRLVVAQDTGGAIRGP
                     VRADLFFGFGAEAGTQAGMMKNDLEMWLLWPKGTPLP"
     gene            complement(7382..9529)
                     /locus_tag="VIV63_02610"
     CDS             complement(7382..9529)
                     /locus_tag="VIV63_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY6123517.1"
                     /translation="MKQTPAAVAQAEINTATAAATSAPKPPVAAKKPYQVVSANGSRE
                     DEYYWLRDDKRENPEMLAYVNAENAYADAMLAHIKPLETKVYNEIIGRLQQDDSTVPY
                     LMNGYWYFRRFETGKEYPIYSRRADAPNAPEEILLNGNELSAGHDFFEIGEIAISPNS
                     KLMAWAEDTVGRRQYVVKVMELATRKIYPIALPNVENNVIWAGDNQTFFYIEKNPETL
                     LGFRVRSHRLDSANHKDVAADPVVWTQDDESFYTQLARTKDEKFLLIHTQSTVSTEVL
                     YADAASKKLEFKVFLPRERDHEYQVEHANGRWVLRTNWQAKNFRIVEVKPGAEGDRTK
                     WTDIVAHRDDAFVDAFDVAKNFLMIEEHSGGLRKLRIRPWPKGEVNPKADVFVQADEP
                     SYTMALDVNREFDSDKLRYTYQSMVTPKTTYEYDFKTTKREMLKREPVLGGYDPANYT
                     TEFAWATARDGAKVPVSIVYAKSTPRNGTAPLLQIGYGSYGSTYDPEFSYLPPSLLDR
                     GFVVVIAHIRGGQEMGRAWYENGKLLNKKNSFTDFIDVTRWLAANKYSDPKRAFALGR
                     SAGGLLMGAIANLAPQDYRGIVTGVPFVDVVTTMLDESIPLTTNEFDEWGNPKEKKYY
                     DYMLSYSPYDNLSAQNYPALFVHSGLWDSQVQYFEPTKYVARLRARRTGDSLVVLRTN
                     REAGHGGKSGRYEHFREYAEQYAFIIDQAGIKE"
     gene            9684..>10029
                     /locus_tag="VIV63_02615"
     CDS             9684..>10029
                     /locus_tag="VIV63_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY6123518.1"
                     /translation="MFTIGNRLNFTALRDLSRGGLVLSISEPAYAAIERGAAAVAQIV
                     KRGEPAYGVNTGFGRLAQTHIPNEQLELLQTNLVLSHAVGVGAPLPAPVVRLTLALKI
                     ATLARGYSGVRRV"
BASE COUNT         1750 a         3093 c         3304 g         1882 t
ORIGIN      
        1 gcgtcaggtt gtcggaaaat ttctgcgtgc ccgtgaaggt ggccgtgaag cgggtgaggg
       61 tcgagtccac gatgctcggc ggtacgccga aaggatcgcg aatgcccgga gcgacgcccg
      121 gcgagtcgat gtggtcgtcg cgatcaaagt agccgagcgc gagcgcgaac gtggcgtcgc
      181 cggcgcgatg cgaaacgccg aagccgaaca cgctttcgtc cgcgtcgcgc ttctccacgt
      241 cgcgaatggc cgcgaattca tagccgccgc tgtcgtcggg gaaaccttcg cgctgggatt
      301 cggagtagcg ccccgaaacg ctcagcgtgg tgaacgcgcc gagctcggta ttgaagttgc
      361 cggacacccg ctgcgactcg aaatgattac cgcgaacctg ctcgccttca ttggtgtcgc
      421 tgaagcccat gttccagtcg ccgccgaccg ccggcccgcc ggccgtgacc gcgatttccc
      481 gcgtgtcgta ggcgccgccc atggcgtcga gcgaggcata actcttcgcg gcgggcacat
      541 gccgggtgac gatgttgatg acgcccgcca gggcatcgcc gccatagatg gcggaataag
      601 ggccgcgcgc gacttccacg cgctccacgt cggcgatgtc gagcgccgac aggtcgaagg
      661 atccgccacg cgcattggtg gggtcgttga cgcgcacgcc gtcgatgagc accagcgtgt
      721 agttaggatc tgcgccacgc acgtagaccg agccggtgcc gccgcggcca cccacctggt
      781 cgacgtgtac gcccgccacg cggcggagca cgtcggcgag tttcgcgggc ttctcgacgt
      841 cgaagtcctc gcgttcgagc acggtgacgt tggccgatat cttgtcgggc gtggtcgcga
      901 cccgagaacc tgtaactacg acttcaggcg taggcgcggg cagggcaaca ccgccgttgg
      961 cggcggcaat aatccataga attgacatag gtgatttgaa ggaaaccggt ttcagagagt
     1021 gggaaatttc ggttagtgta gctgcacaca agggggtttc atgtcgagat ttctgtgccg
     1081 ttacgcggca ggcgtactga ccatcgccgc gctcgcaagt ttccatatcg cagaggctca
     1141 gaacgccgtg ccgcccgagg cggcgtttcg cgaaaacccc gcctggacgg tggaagacgg
     1201 cgcgctcacg acaaaagtcg cagccccgga cagaggtctg gtcacgcgcg tgacactgtc
     1261 ggacagcatc tcgtcgttcg aatacaaagc gcccgtgggc tcacgcgccc acttcgtggt
     1321 catgggccga tacgcctttg aacttgtggg taatggcaat tggcaatcct ttgccctgcg
     1381 cttccggggg ccgcgtttcg actccggctt caccaagctc gacaacgctt tcgcgctcga
     1441 tgtgcgcaac ggtgccgaaa cacgccgtag cgtcttcttc gaagggccca gcgcagtcgc
     1501 catctggcag ggcgaagaca agcgtggacc ttcgttcatc gtggcccagg ggccgttcgc
     1561 agtgcgcaac gcgcgccacg aggccgcgga tttcgatcag ctgacgctgc ccaaggccgc
     1621 cggtggcgac accaatgaga aagacctgcg cgacctcgtc gcgctcggca aacagacgtt
     1681 cgaggacgtg ggctgtcacg cgtgtcatct cgtcgacccc agcgacaccg ccgtgagctc
     1741 gggcccgaat ctcttcggcc tcatccgcgg cgaaccgcgc acgcgcgaag tggtggaggg
     1801 tggtgaaggg catcgcttcc agatcaaggc ggggcgcgaa tacgtgcgca actcggtgcg
     1861 tatgccctcc gagcagctgg ccgtggcgga gagtggcccc acgaagggac aagcctatct
     1921 acccgtgatg ccgccgttca ccaaagacgt gctcggcgat gcgcagatcg acgccatcag
     1981 cgactacctg gccacgctga atctgccgca ggatcgcggt ccggtggtca agctcgcggg
     2041 cctcacgccg gagccgccgt acgatcccat gaaggactcc ctgcagtggc tggtggccga
     2101 caccgtgcga ttgcagcgcg ggcctctggc cggtgtgtcc ggccgaagca tccacgtcgg
     2161 caatccgaat ggcgtgaact acaccttcga tccgcggctg ctggccatcg tgaagatctg
     2221 gcagggcggt ttcctcgaca tgaccggcga gctcaccaac cgcggcaatc gcggactcgc
     2281 catcggctac gaaagccgcg agatcagttt cggcgaacgc gcctacctcg tggcgccgct
     2341 caacgcggcg ggtgctcccg tcgatttcac gttcaaggaa ggcaagttcg gcgataccgc
     2401 gacgttcaag tcctggctct acagcaagga agatcagctt gcgctgattg cgaaggccga
     2461 tgcccagttc ctgggttaca cgcgcgactc gaagaacaag acggccgcgc cttcgttcaa
     2521 gtaccgcgtg ggaaagaaca cggtcgatgt caccacgtcc atctccgagc gtggcgaagc
     2581 cgcgattcgc gtcgcgggca agctggccac ggagcagagt ttcgtgctga atcccgcgct
     2641 gctcaccggc gtcacggtca gcggcggcag cctgagcggc gatcgctgga cactgcccgc
     2701 gggtagtgcc aatgccacgc tgagcggcaa gatcgccgtg gcgcccgcgg cctggcgccc
     2761 tgccgcgtct aactacgcct acgaacgcaa gccgctcgag cgcacgccgg ccaccgccac
     2821 gctgccggcc ggatacagca tcgagaacta ctactcgccc aaggacaact acggccgcga
     2881 tcagttgttc gaagccctgg gtctcgcggt cgccaaagat ggcaccgtgg tcgtcgccac
     2941 gcgtaccgcc ggcatctggc ggctggtcaa gggcgagtgg cgtttgttcg cggaaggtct
     3001 gttcgacagc ctcggcgtcg tgatcgaaga cggcaagggt ctcgtcgtcg tggccggaca
     3061 gaaggccgag ctcacgcgca tcagcgacac caatggcgac ggcattgccg acaagtacga
     3121 gacgctgttc gatgcgcact cgtaccacgg caactaccac agctacatgc atggcccggt
     3181 caagggcggc gacggggctt actacttcgc gctcaacctc gtgcacgacg gctcgggagc
     3241 ggcgtacacg gcgggcggca acgtcatggg tacctggggc ggcttcaatg gttgggccgt
     3301 gcgcgtcgaa cccgacggaa agttcgagct gttctcgaag ggactgcgca gccccgcgag
     3361 cctgggtgtg ggacccgacg gacgcgtctg gtatgccgac aaccagggcg atttcgtcgc
     3421 cacctcaaaa atgttcgtgc tcggcaagga caagttctac ggccatccgg ccggtctcgt
     3481 cgatctgccc ggaatgacgc cggactcgcc cgacatcgcg tacgacaagt gggccgatcg
     3541 tcgtgaacgg cccatcatca tgttcccgca caaccgcgtc gccaactcgc ctggcaatcc
     3601 ggcgtgggat accacgcgcg gcaaattcgg tccgttcgcc gggcagatgc tgatcggcga
     3661 ccagacgcag tcgaacctgc tgcgggtggt cgtgcagaag gtcggtgacg tcgaggaagg
     3721 cagcgtcatg ccgttcatgg aaggactcga gtccggcgtc atgcgtccgg tgttcctgcc
     3781 cgacggcagc ctgttgttag gccagaccgg ccgcggctgg caggccaagg gtggaaaggt
     3841 cgccgcgctt caacacgtgc gctgggacgg aaagacggtc gcgccgcaga tcaaggccat
     3901 ggtggcgaca ccggacggct tccgcgtcga tctcacgcag ccgctgggca acggcgtgag
     3961 cgaagcaatt ctgaagtcgg cgctgacggt ggaatcgtgg gtgtatcgcg atgcgccgga
     4021 ctacggctca cccgagctcg atctgcatgg agaagacatc gcgtcgttca ccgtgagcgc
     4081 ggatcgcaag tccgtcgccg tgaagctgct gtccatcgag cagaagaccg tgcatccgcg
     4141 gcagaccagc cgcatctacc acctcaagct cgcgtcggcg acgctgttcg atgcgaacgc
     4201 gccggcggcg ctcaacgcct actacacgct gtctcggttt ccagcggcga agtagccgga
     4261 aaatggggac actccccatt tgctagcaaa aagggccgca cctcattcag gtgcggccct
     4321 ttgttcatca ggcagctttc ttcgtcaggc cctcagcggc catggcttcc tgcacgtgcg
     4381 gacgcgctgc gacgcgcgcc tggaattcct tcagcgccgg ccacggagtc agatcgatgc
     4441 cgagcgggtt cgcccagttc accaccgtga agaggtacgc atcggcaatc gtgaaggtgt
     4501 cgcccgtcag gtacttcttg ccggcaagct gcacgttcag ccagtcgaac ttcttcttga
     4561 ggttggtgag cgcgtaatcc ttcaccacct gttcgttcgc cggattccac agcggcgaga
     4621 agctcttgtg cacctcgctc gtgatgtagt tgagccattc ctgcactcgg tagcgctcga
     4681 gcgtgccgtt cttcggcgcg aggttggtct caggcttctg atcggcgagg tactgcatga
     4741 tggccacgcc ttcggtgagc acctggccat tgtcgagctt caacgcgggc accgcgccct
     4801 ttgaattgat ggcgaagtag tcagagccat cttccagctt cttcgtcttg gtgtcggcgc
     4861 gcgtaaggtc gagcggcagg ccggcttcac gtgcggtgat gtgcgaggcg agtgagcagg
     4921 cgccggggga gaaatacaac tgcatgacat actccattgc gtggtcttgg gttcgggagg
     4981 ccggcattct ggacacccac tccccagcct acaagtactt accttctggt aagtaccccg
     5041 tagggctttc gggctagtac gtaccttttg gtaagtatca ccctcgtgtt atggtcccgc
     5101 gcatgccaaa agcccacgga tccgccaacg tcttcagctc ccggtgccca tcgcgccgcg
     5161 tcatggccgt gcttgcggaa aaatggacgc tgctcatcgt cgcgcagctc gccaacgggc
     5221 ccatgcgcac cgcggaaatc cgccggggcg tcgacggtgt ttcggagaag atgctcatcc
     5281 agaccttgcg caagctcgaa gccttcggcc tggtgagccg caagagttat ccggaagtgc
     5341 cgccgcgcgt ggactatcgg ctgacgccgc tgggccgctc gctctcgcgc ctggccggcc
     5401 tgttcagccg gtgggtcgaa cgcaatgtct cgaacctgct caaggcggag caggacgcgg
     5461 cgtgaaattg atgctcggtt gagaaaaact catcgatgaa atgaccacca ggtgacaaac
     5521 aatcgcaccg cggtggcgcg atgtaaaaaa atcgcgcgca cttgaagtgc gtgaatgacg
     5581 cagttcgcaa agcgcttatt ttccagtagc aagacgtgtg cggcgccagt aatttccgcc
     5641 cacatgaaaa ccattacgaa tagagcattc tccctaggtg ccgtgatact gatggcggtc
     5701 aacgccgcga cggccgcaga gaaggttgcc actgaagagc tggccgtcct gcaggcgatg
     5761 gccgaatcga tcaaccgtga agcctcgcgt ccgttcgagc gcctctattt cgagtcggag
     5821 ttcgaaggcg cgccccatgt cgcgagctcg ctcgcgaatc cggatcgcac gaagttctgc
     5881 ggactctcgc gcgaagaagc gatcgaactg gtttcaacgc tccaggcgct cagcaccgac
     5941 cccgtggaga tcgacgatga agtcgcgaag tcggctggcc tgaagatagg ccacaagaag
     6001 aacccgcgat ttccctacgt catcttgtcg cgcgtcgtct tcggtccggg caacaagtcg
     6061 gcgtgggtcg cggtcgagct gaatggatcg tcgggcgccg tcatgcggct cgacaaggtc
     6121 ggcggagcgt ggagcaagac cgctcgttgc ggcggctggc tcaagcccga ggattgacgc
     6181 cactacggta gcggtgtgcc tttcggccag agcaaccaca tttcgaggtc gttcttcatc
     6241 atgccggcct gcgtgccggc ttcggcgccg aatccgaaga acaggtcagc gcgcaccggt
     6301 ccgcgaatgg cgccgccggt gtcctgcgcg acgacgagcc gctgcagcgg caggccgctt
     6361 ccgggcgcgg tggtcgagat gaacatgggc gcgccgagcg gctgaaactt cgggtccacc
     6421 gcgatggatc gtccggccgt cagcggcacg gcgagcgcgc ccttcggacc caccgacgca
     6481 tcgtccagcg gagcttcgcg aaagaacacc acgctcggat tggtgtcgag cacttcgtgc
     6541 aggcgctgcg gattggcggc cagccaggcg cggatggcgg acatgttcac ctgatcgatg
     6601 gtcatgacgc cctggtcggc gaggtatttg ccgatggacc ggtagggatg cccgttctgg
     6661 tcctcgtatt gcagccggat ggtctcgccg gtagtcagct ggacgcggcc ggacccctgc
     6721 acctcgagca tgaacgcgtc gagggcattg tctatccaca cgatttcatg gccggcgagg
     6781 ccgggcgcct cgcgtgtcgc cgcacggctg tagtagggca ccaccttgtg gcccacgacg
     6841 cggcctcgca cgcgcttgcc cttgagttcg gggatgacct cggcaagatc gacgatgagc
     6901 agatcgtcgg ggcgccggta cagcggcgtg gcgaactgtt cggagcgttc gcgcgagccc
     6961 atgagcagcg gttcgtaata gccggtgatg agcccggtct tctcgcgctt gccgtcggtg
     7021 acgcgcagca cgtgataggg ctgcaggttc tgctcgagga agccgcgcac gaattcggaa
     7081 tcgccggggc tggcggcgac gacggagccg cagaacgatt gccatgcggg ccgcgagtgg
     7141 atgacgctgc aggacgcgac gatggccggc caggcggcca gcgggtcatc ggcgcgccag
     7201 cccggaagtt tcgaccagct cactggctcg aagcgtgccg tcgcggggcc gggcgtcgcg
     7261 accttgcgcg acgtctcgca ggccgcaagc agcgccatgg cgccgcagag aacgattcgc
     7321 gcgatctgcg agatcgccgg cgaggtccgg ccccatttcc cggaaatggg gactgtcccc
     7381 attattcttt gattccggcc tgatcgatga tgaaggcgta ctgctccgcg tattcgcgga
     7441 agtgttcgta gcggcccgac ttgccgccgt gcccggcctc cctgttcgtg cgcagcacca
     7501 cgaggctgtc gcccgtgcgc cgcgcacgca ggcgcgcgac gtatttcgtg ggctcgaagt
     7561 actggacctg gctgtcccac aaccccgagt ggacgaacag ggcggggtag ttctgcgcgc
     7621 tcaggttgtc gtacggcgag tacgacagca tgtagtcgta gtacttcttc tctttcgggt
     7681 tgccccactc gtcgaactcg ttggtggtga gaggaatgct ttcgtcgagc atggtggtga
     7741 ccacgtcgac gaacggcacg cccgtgacga tgccgcggta atcctgcggc gcgaggttcg
     7801 cgatggcgcc catgagcagg ccgcccgccg accggccgag cgcaaaggcg cgcttggggt
     7861 cgctgtattt gttggcggca agccagcgcg tgacgtcgat gaagtccgtg aacgagttct
     7921 tcttgttcag cagcttgccg ttttcatacc aggcgcggcc catttcctgg ccgccgcgga
     7981 tgtgcgcgat gacgacgacg aatccgcggt cgagcagcga cggcggcagg tacgaaaatt
     8041 cgggatcgta ggtcgagccg taagacccgt acccgatctg cagcagcggc gccgtgccgt
     8101 tgcgcggcgt ggacttcgca tagacgatgc tcaccgggac tttcgcaccg tcgcgcgccg
     8161 tggcccaggc gaactcggtg gtgtagttag ccgggtcgta accgcccagc accggctcgc
     8221 gcttgagcat ttcgcgtttc gtggtcttga agtcgtattc gtacgtggtc ttcggtgtga
     8281 ccatggactg gtacgtgtag cgcagcttgt cgctgtcgaa ctcgcggttc acgtcgagcg
     8341 ccatggtgta gctgggctcg tcggcctgca cgaacacatc ggccttcggg ttgacttcac
     8401 ctttcggcca gggacggatg cgcagtttgc gcaagccccc ggagtgttcc tcgatcatga
     8461 ggaagttctt cgcgacgtcg aacgcgtcga cgaaggcgtc gtcgcgatgc gccacgatgt
     8521 ctgtccactt cgtgcgatcg ccttcggcgc ccggtttcac ctcgacgatg cggaagttct
     8581 tcgcctgcca gttggtgcgc aggacccagc gcccgttggc gtgctccacc tggtattcgt
     8641 ggtcgcgttc gcgcggcagg aaaaccttga attcgagctt cttcgacgcc gcatcggcgt
     8701 acagcacctc cgtcgagacg gtgctctgcg tgtggatgag caggaatttt tcgtctttcg
     8761 tccgtgcaag ctgcgtgtag aagctttcgt cgtcctgcgt ccacaccacc ggatcggcgg
     8821 cgacgtcctt gtggttggcg ctgtcgagcc tgtggctgcg cacacgaaac ccgagcagcg
     8881 tctccgggtt tttctcgatg tagaagaacg tctggttgtc gccggcccag ataacgttgt
     8941 tctccacgtt cggcagtgcg atgggataga tcttgcgcgt ggcgagttcc atgaccttca
     9001 ccacgtactg gcgacggccc acggtgtctt cggcccaggc catcagcttg ctgttggggc
     9061 tgatggcgat ttcaccgatt tcgaagaagt cgtggccggc agacagctcg ttgccgttca
     9121 gcaggatttc ttcgggtgca ttcggcgcgt ccgcccgtcg cgaatagatg gggtattcct
     9181 tgccggtttc gaaacggcgg aaataccagt agccgttcat gagataggga acggtggagt
     9241 cgtcctgctg cagccggccg atgatctcgt tgtagacttt ggtttccagc ggcttgatgt
     9301 gcgcgagcat ggcatccgca tatgcattct cggcgttcac gtacgcgagc atttccgggt
     9361 tctcgcgctt gtcgtcgcgc agccagtagt actcgtcttc gcggctgcca ttggcggaca
     9421 ccacctggta gggcttcttc gcggcgacgg gcggcttggg agccgaggtg gcggcggcag
     9481 tggcggtgtt gatttccgcc tgggcgacgg cggcaggtgt ttgcttcatg ttcgtattgc
     9541 aggctgtcag tgaaacgagg gtggcaaagg caaggaggcg cgggacagcg aaacgggagt
     9601 ccatgacgag aaccttgttc gggtgaggcg cgtatcttac cgagggactg aactggcggg
     9661 ggcagttgag gcatcatgcc gcgatgttca ccatcggcaa tcggctgaat ttcaccgcct
     9721 tgcgcgattt gtcgcgcggc ggtctcgtgt tgtcgatttc ggaacccgcc tacgcggcca
     9781 tcgagcgcgg cgcggccgcg gtggcgcaga tcgtgaagcg cggcgaacct gcttatggcg
     9841 tgaacacggg ctttggccgg cttgcgcaaa cccacatccc caatgagcag ctcgaattgc
     9901 tgcagaccaa cctggtgttg tcgcatgccg tgggcgtcgg ggcgccgctg cccgcgccgg
     9961 tggtgcgcct cacgctggcg ttgaagatcg ccacgctggc gcgcggttac tcgggcgttc
    10021 gccgcgtac
//
LOCUS       DATDEU010000109        13114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1771378, whole genome shotgun sequence.
ACCESSION   DATDEU010000109 DATDEU010000000
VERSION     DATDEU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771378"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VIV63_02620"
     CDS             <1..544
                     /locus_tag="VIV63_02620"
                     /inference="COORDINATES: protein motif:HMM:NF023287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HEY6123519.1"
                     /translation="GALDVPAPSNYRKPWELARERRSPMRWYALAASVVVALTIAGGT
                     WLHLKRDVLFAELVKHAHGESNVMVATDKRASEDKVRTTLAKAGARLQSAIPVSTVRT
                     CKVRGVVAPHLFVQTPDGPVQLLLLTEHRFLMSHATDKQGYHAELVPVGNHSVAVIAA
                     TDAAVQQGVRMVRQSIDWSP"
     gene            697..3501
                     /locus_tag="VIV63_02625"
     CDS             697..3501
                     /locus_tag="VIV63_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123520.1"
                     /translation="MLNDHWMIRRHVRAALGLLAGSLALNAQAQEATASAPQEEIEEV
                     VVTGFRASLNAALDDKRASSAAIDSIRAEDIAKFPDSNLAESVQRIPGVSIARDAGEG
                     RNISVRGLGPQFTRVRINGMEAQSTTGGTDSSGGSNRNRQFDFNVFASELFSGITARK
                     TSSAEVEEGSLGATIDLRTARPFDYPGFTLVGALKGAYNDLAEEVDPRATFLVSNTFG
                     DGTFGVLFSAAYSERNAFEEGSSTVRWDSGSSVGGFNAASTPTGVTLAQANAATTFHP
                     RIPRYGRLTHQQERLGLTGSLQWQVNDANLLSFDALYSNYDAKRDENYLEAFSFSRNA
                     AQGGKPATRILAGEMDANGTLVYGLFDDVDIRSESRYDELSTKFGSYTLTATHELGER
                     WSMSELAGYSRSDFNNPIQTTITLDRPNTDGYSWDFRGNDRLPAFNYGYDVNNPANWT
                     FGTFPGGSSEIRIRPQGVDNTFQVGKIDFKFVATDLFALKFGADYKKYDFVSFESRRA
                     SETTVPALPAGTTLADLSTLLTGFGSGLDLPANTDLSWLMPDLDAFARTFDIYSNTGM
                     FVLGSINNAAARGNNRSVTEQDEGLYLQADFSFDWGIPVRGDLGVRYSRTKQTSQGYL
                     PAGGAPVLLTAVHEYSDVLPSLNLVGEVTPDLLVRFAAAKVMTRPALGQVTPGGTTSL
                     VGNLGVATGNPYLDPTRATTYDAAVEWYFDEGALLSGAIFYKDIKTFVQTLVENMPFN
                     QSGYPLDLLAGTTLTGTEVFAFSNPVNTPGGPLKGFEINYQQPFKFLPGAWSNFGVLL
                     NYTYVDSEIDYVTSMSGATPPVTNDLVNLSKHAWNGTLYYEGGGFSARASASYRDRFL
                     TAVPAANAPTIQDADGTNESLNVDLSTSYEVNEHLTISLEGLNLTDEANDQFTDTDAN
                     RVVVYTHTGRQVFVGARYKF"
     gene            4043..7081
                     /locus_tag="VIV63_02630"
     CDS             4043..7081
                     /locus_tag="VIV63_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123521.1"
                     /translation="MLLGTAPAFAQDQGTEELEEVVVTGLRGSMIASMNIKREAVGVV
                     DAISAEDIGKFPDTNLAESLQRISGVSIDRVAGEGSRVTSRGFGPGFNLVTLNGRQMP
                     TADVVVVGSGGDGEYGTFTSRSFDFSNLASEGVTGLEVHKTGSALTPSGGIGATINVR
                     TMRPLTAGNKASIGAKAMFDTSVETGSEVTPEVSGIYSWANDSESFGVSLFGSYQKRE
                     SASVGASNQDWNVERLDSFLNPGNGRVRADNPLTTTVDETTIINNRPTGNPLISYPNN
                     SDYFFSEIERERINGQAVIQFRPVDSFTITADYLFAQNENDEMRSTQGNWFNRPFARV
                     DFDTGTEVPTAVFLQDTLSGPKDIAWGQQLRATKDELESIGLNLRWDVSDSFALEFDG
                     HTSESKSDPNGPLGRTSFDFGTGAAAITAHTLDLTTGFPIQQFTYTDTNGNNNGIIDI
                     ADVSSSVGRTAMQSQVHDIDEFRLDAEFTFNDSMKLSGGVDYRTSSMTQERLVTAQVL
                     GDWGVTAPGDIESQAPGVLEAYCLSCLYDDFSPGLGATAFRGNAADLMSALAPYYLDL
                     GHPIQVWNNDHNSVDEDITSIYAEFAWNGELGGRKASLVAGLRYEETDVTAVSVLAVP
                     DGVIWTADNDFASDFSSGTVLPLSDDASYDNFLPNLDFSVELLDNVIARASWSKTIAR
                     ADYGQLFASESAGTPPRATALGGIATGSSNNTALVPLESTNIDISLEVYYGEASYISI
                     GFFNKDVENFIGTGQITRPMFDLRDVSSGAPGTRSGAASTALAGIPGALRNDVNMFVM
                     TAMIDNPTAFPDPTATFLANSTNGELNQVFADQIFAAYNLQSNASDPLFQYQVSRPIN
                     QNDANIHGVEFAFQHFFGESGFGIQGNYTFVDGDVAINVAGDPGVDQFALEGLSDTAN
                     ATLMYEKYGFTARISYNWRDEFLNQVSRGGYRNPTFVNAFEEIDLNLSYDINDALAVS
                     FEAINLTGEDLRTRSRTDVDYWFAQELHARYLLGVRYKFN"
     gene            7165..8196
                     /locus_tag="VIV63_02635"
     CDS             7165..8196
                     /locus_tag="VIV63_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY6123522.1"
                     /translation="MAEIARKTQVITGVAPDRIPYAELLEAGQPSILKGVARDWSLVR
                     NARESPEKAIAYLKKFCGVTPITVFLGPPEIAGRFFYNEKVTGFNFAAHKVPLAETLD
                     RLQLLMSDEHAPAVYVGSTDIDLYLPGLRAENDLPLNDPMFTHNRPLVSIWLGNRTVA
                     SAHFDSTHNLACCVAGHRRFTLFPPDQVHNLYPGPLEPTPGGQVITMVDFNAPDFERF
                     PRFREALAAGQVADLEPGDVLFYPAMWWHHVEALDPFNALINYWWTTTPRYMDSPQTT
                     LMHALLSLRDLRPQEKSAWRELFDYYVFGTAGRAAEHLPESARGNLGPMDDTKARRLR
                     AFLLSRLNR"
     gene            8216..9703
                     /locus_tag="VIV63_02640"
     CDS             8216..9703
                     /locus_tag="VIV63_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6123523.1"
                     /translation="MTSQSVKKVVITGGGTAGWVTAAALAHQFSSLLDITLVESEEIG
                     TVGVGEATFPSILAFHRLIELDEQKFMRAAKASFKLGISFENWGGLGDRYIHAFGTVG
                     KSTWMGDFQHFWLAAREGGFAGDLGEYCFERQAALAGKFATSEAVKINYAYHFDAGLY
                     AAYLRQISEPKGVRRIEGKIKSVEQHPESGFVQALVLESGQKVEGDLFVDCSGFRGLL
                     IEQTLKAGYEDYSPWLRTDRAFAVQTPSGKDIPPYTRAIARQAGWQWRIPLQHRVGNG
                     MVFSSQYQSEDEARATLLANLEGEALFEPRLLKFKAGRRLKAWDKNVVSMGLAGGFIE
                     PLESTSIHLIQIGVTRLMQLFPFAGIHPALVNRYNDQSRIEYERIRDFIILHYKLTER
                     DDTAFWRDCRDMPIPDTLAERIELFRESAMAYQATDEMFRVDSWMQVMIGQRLIPRGY
                     HHMGRMLGPERLRKALDTLKGSISGAVARMPTHREYLNSYCAAGE"
     gene            9957..10331
                     /gene="rpsL"
                     /locus_tag="VIV63_02645"
     CDS             9957..10331
                     /gene="rpsL"
                     /locus_tag="VIV63_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY6123524.1"
                     /translation="MATTSQLIANPRRVNKEKTKVPALGASPQKRGVCTRVYATTPKK
                     PNSAMRKVARVRLVNGYEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGTL
                     DCAGVGNRKQGRSKYGAKRAKK"
     gene            10343..10822
                     /gene="rpsG"
                     /locus_tag="VIV63_02650"
     CDS             10343..10822
                     /gene="rpsG"
                     /locus_tag="VIV63_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY6123525.1"
                     /translation="MSRRTAAPVRHILPDPKFNNDMLAKFMNVVMKSGKKSAAERILY
                     GAIDRIVEKSGKPATDAVAMLSTALDNVKHVVEVKSRRVGGATYQVPVEVRAQRRQTL
                     AMRWVIEAARARSETSMAFRLAHELMDAAENRGAAVRKREDTHRMAEANKAFAHYRW"
     gene            10847..12934
                     /gene="fusA"
                     /locus_tag="VIV63_02655"
     CDS             10847..12934
                     /gene="fusA"
                     /locus_tag="VIV63_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY6123526.1"
                     /translation="MPRNTPIERYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAAVMDYMEQEQERGITITSAATTCFWKGMDKSFPEHRINIIDTPGHVDFTIEVERS
                     LRVLDSAVALFDSVAGVQPQSETVWRQMNRYNVPRIAFVNKMDRAGADFLRVCEMIRA
                     RLRANPVPIQLPIGAEDSFEGVVDLIKMKAIYWDMETQGMKFEFREIPAPLKAQAEEY
                     RAKMIEAAAEGDDALTDKYLGGGTLSDDEIKRGLKARALKNEIVLCMCGTAFKNKGVQ
                     ALLDAVIEFMPSPIELPPTKGLSEKGETITRTAEDGQPFSALAFKILNDPFVGNLTFF
                     RVYSGTLNSGDTVYVPSRSKKERIGRLLQMHANERSEIKEVHAGDIAAAVGLKDVYTG
                     DTLSDLEKVITLEKMTIPIPVISVAVEPKTQADQEKMGVALQRLAKEDPSFRVSTDEE
                     SAQTIISGMGELHLEIIVDRMRREFKVEANVGKPQVAYRETIRAKIESEGKHIKQSGG
                     RGQFGHVWLKIEPNEQGKGNEFVNGIQGGSVPREFIPAVEKGIKEALETGVMAGYPVV
                     DTKVTLFDGSYHDVDSDEISFRMAAIFGFKDGFRKAKPVILEPIMKVEVVTPEDYMGD
                     VIGDLNRRRGQIQGMEDTPAGKTVTAEVPLSEMFGYATNVRSMSQGRATFTMEFAKYM
                     EVPPTIADGIINK"
     gene            12965..>13114
                     /locus_tag="VIV63_02660"
     CDS             12965..>13114
                     /locus_tag="VIV63_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6123527.1"
                     /translation="MSKEKFERNKPHVNVGTIGHVDHGKTTLTAALTKVMAETNGGSY
                     MAYDQI"
BASE COUNT         2642 a         4152 c         3992 g         2328 t
ORIGIN      
        1 gggagcactc gacgtgcccg cgccatcaaa ctaccgcaag ccctgggagc tggcgcggga
       61 acgccgttcg ccgatgcgct ggtatgcgct ggccgcgagc gtcgtggtgg cgctgacgat
      121 cgccggtggc acgtggctgc acctcaagcg cgacgtgttg ttcgccgagc tggtcaaaca
      181 cgcgcatggc gaaagcaacg tcatggtcgc caccgacaaa cgcgcgagcg aagacaaggt
      241 gcgtacgacg ctggcgaagg cgggtgcacg cctgcagtcg gcgatccccg tgtcgacggt
      301 gcgcacctgc aaggttcgcg gtgtggtcgc gccccacctg ttcgtgcaga cgccggacgg
      361 cccggtgcaa ctgctgttac tcacggaaca tcggttcctc atgtcccatg caaccgacaa
      421 gcaggggtat cacgccgaac tcgtccctgt cggaaaccac tcggtggccg tcatcgccgc
      481 caccgatgcg gccgttcagc agggcgtccg catggtccgc cagtcgatcg actggtcgcc
      541 gtaagttttt cccgaagtcc ccggtcactc actgcaggac ggtgatccga cccatgccgc
      601 ttgcgcggca cggcgtcgta ttgctcatca aaaacgggtg ccgctcgtca gccggattgc
      661 taccggtgtc atcggcccgc ttcaggggga cacaacatgt tgaatgacca ttggatgatt
      721 cgacggcacg tgcgcgcggc attgggcctg ctcgcgggca gcctggcgct caatgcgcag
      781 gcacaggaag cgaccgccag cgcgccgcag gaagaaatcg aggaagtggt cgtgacgggt
      841 tttcgcgcca gcctcaacgc cgcgctcgac gacaagcgtg cttcgtcggc ggcgatcgac
      901 agcattcgcg ccgaggacat cgcgaaattt cccgattcga acctcgccga atccgtgcag
      961 cgcatccccg gtgtctccat cgcgcgagac gcgggtgaag gcaggaacat cagcgtgcgc
     1021 ggcctgggtc cgcagttcac gcgcgtgcgc atcaacggca tggaggcgca gagcaccacc
     1081 ggcggcaccg acagttccgg cggctccaac cgcaatcgcc agttcgattt caacgtattc
     1141 gcgtcggagc tgttcagcgg tattaccgcg cgcaagacct cgtcggccga agtcgaagaa
     1201 gggtcgctcg gcgcgaccat cgatctgcgc accgcgcggc ctttcgacta ccccggcttc
     1261 acgctcgtgg gcgcgctcaa aggcgcctac aacgatctcg ccgaggaagt ggatccgcgc
     1321 gccacgttcc tggtgagcaa cactttcggt gacggaacct tcggcgtgtt gttctcggcg
     1381 gcatattccg aacgcaacgc gttcgaagaa ggcagcagca cggttcgctg ggattctggc
     1441 agcagcgtcg gtggcttcaa tgccgcatcg acgcccacgg gtgtcacgct cgcgcaggcc
     1501 aatgcggcga ccacgttcca tccgcgcatt cctcgatacg ggcggctcac acaccagcag
     1561 gaacgattgg gcctcacggg ctcgctgcag tggcaggtca acgacgccaa cctgctttcc
     1621 ttcgacgcct tgtactcgaa ctacgacgcg aagcgcgacg agaattacct cgaggcgttc
     1681 tcgttcagcc gcaatgcggc gcagggcggc aagcccgcga cgcgcatcct cgccggtgag
     1741 atggatgcga acggcacgct ggtctacgga ctgttcgacg acgtcgacat ccgttccgaa
     1801 tcccgttacg acgagctctc gacgaagttc ggctcgtaca cgctgaccgc cacgcacgaa
     1861 ctcggcgagc gctggtcgat gagcgaactc gcgggttact cgcggtcgga cttcaacaac
     1921 cccatccaga ccacgatcac gctggaccgc cccaataccg acggatactc ctgggacttc
     1981 cgcggcaacg atcgtctgcc cgcgttcaac tacggctacg acgtcaacaa cccggccaat
     2041 tggactttcg gaacgttccc gggcggctcg tccgaaatcc gcatccggcc gcagggcgtc
     2101 gacaacacct tccaggtcgg caagatcgat ttcaaattcg tcgccacaga cctgttcgcg
     2161 ctgaagttcg gcgctgacta caagaagtat gacttcgtgt cgttcgaatc gcggcgcgcc
     2221 tcggagacca cggtgcccgc gctgccagcg ggaaccacgc ttgcggacct cagcaccttg
     2281 ctgacgggct tcgggtccgg gctcgatctg ccggcgaaca ccgacctgtc ctggctcatg
     2341 ccggacctcg atgcattcgc gcgcaccttc gacatctaca gcaatacggg catgttcgtc
     2401 ctcggcagca tcaacaacgc cgcggcgcgc ggcaataatc gcagcgtcac cgagcaggac
     2461 gagggtctct atctacaggc cgacttcagt ttcgactggg gcatcccggt gcgcggcgat
     2521 ctcggtgttc gctactcgcg cacgaaacag acttcgcagg gatatctgcc tgccggcggc
     2581 gcgcccgtgc tgctcacggc ggtacacgaa tactccgacg tgttgccctc actcaatctc
     2641 gtcggagaag tcacgccgga cctgctggtg cgcttcgccg ccgcgaaagt gatgacgcgg
     2701 cccgcgctcg gccaggtgac gcccggtggc acgaccagcc tcgtcggcaa tcttggcgtc
     2761 gccaccggca atccgtacct ggatcccacg cgcgcgacga cctacgacgc cgcggtggag
     2821 tggtacttcg acgagggcgc actgctctcg ggagccatct tctacaagga catcaagacg
     2881 ttcgttcaga cgctggtgga gaacatgccg ttcaaccagt cggggtatcc gctcgacttg
     2941 ttggccggca ccacgctcac gggcacggaa gtgttcgcgt tcagcaatcc ggtgaacacg
     3001 ccgggcgggc cgctcaaggg gttcgagatc aactaccagc agccgttcaa gttcctgccc
     3061 ggcgcgtgga gcaatttcgg agtgttgctc aactacacgt atgtcgattc ggaaatcgac
     3121 tacgtgactt cgatgagcgg cgcgacaccg ccggtcacca acgacctggt gaacctctcg
     3181 aagcacgcgt ggaatggaac gttgtactac gaaggcggcg gattcagcgc gcgcgcatcg
     3241 gcgtcctatc gcgaccggtt cctcactgcc gtgcccgcgg cgaatgcacc gacgatccag
     3301 gatgccgacg gcaccaacga atctctcaac gtggatctgt cgacgtccta cgaagtgaac
     3361 gagcacctta cgatctcgct cgagggactc aacctcaccg acgaggcgaa cgatcagttc
     3421 accgacaccg atgcgaatcg cgtcgtcgtc tacacacaca ccggccgcca ggtgttcgtg
     3481 ggcgcgcgct acaagttctg aagatcgatg ttgccccggt tcctcttccg gggtgggcgc
     3541 cgggccggtc cacgccttcg gcccggcgcc attttttatg actgtcctgt tcgagacatg
     3601 agggcccgca agggattacc ggcccctttt gcttttttca cttcgacagt gcgcgcctcg
     3661 tgtctcgtgt tgcgttgcag tcggacacaa gacacaacgc gcgacgctgt ggcgctctaa
     3721 ccactgtgca catttttctg catcgttacc gcggaaaaag agttacttcg cgcgcaacag
     3781 tcacatagta tccggcccga gggggacgcc gcgaagggac tggttgcgta cacggctcgg
     3841 ggtatgcagc gagaaggagc ggagttcgcc ggaacaagag tgcggtcaat agttgaaaga
     3901 ctaaagacgg gagcatctca ctatggcgag gaatactcag ttgcctggca acgggaattt
     3961 tttgcaatcg gatctcaaga gggcgattca gaaagtatta agcagtaagc aggaagcgtt
     4021 gctcggcatc gtcggtggag cgatgttgtt gggtacggct cccgcgtttg cccaggacca
     4081 gggcaccgaa gagttagaag aggtcgtcgt caccggcctg cgcggaagca tgatcgcttc
     4141 catgaacatc aagcgcgaag cggtgggtgt cgtcgacgcc atcagcgctg aagacatcgg
     4201 caaattcccc gacaccaatc tcgcagaatc cctgcaacgc atttcgggcg tatccatcga
     4261 ccgtgtcgcc ggcgaaggtt cgcgcgtcac gtcgcgaggc ttcggtcccg gtttcaacct
     4321 ggtcacgctg aacggccggc agatgccgac cgccgacgta gtagtggtgg gttcaggcgg
     4381 tgacggcgaa tacggcacgt tcaccagccg ctcgttcgac ttctcgaacc tggcttccga
     4441 aggtgtcacg ggcctcgagg tccacaagac aggcagcgcg ttgacgccct cgggcggcat
     4501 cggtgcgacc atcaatgtca ggaccatgcg gccgctgacg gcgggcaaca aggcgtcgat
     4561 cggcgccaag gccatgttcg acaccagtgt cgagactggc agtgaagtca cgcccgaagt
     4621 cagcggcatc tatagctggg cgaacgactc cgagagtttc ggtgtgtcgt tgttcggcag
     4681 ttatcagaaa cgtgaaagcg cctcggtggg cgcgtccaac caggactgga acgtcgagcg
     4741 tctcgactcg ttcctgaatc cgggcaatgg ccgcgtgcgt gccgacaatc cgctgacgac
     4801 gacggtcgac gaaaccacga tcatcaacaa ccggcctacc ggcaacccgc tgatcagcta
     4861 cccgaacaac agcgactact tcttctccga gatcgagcgc gagcgcatca acggacaggc
     4921 ggtgatccag ttccggccgg tggattcatt caccatcacc gcggactacc tgtttgcgca
     4981 gaacgaaaac gacgaaatgc gttcgacgca gggcaactgg ttcaaccggc cctttgcgcg
     5041 cgtggacttc gataccggga ccgaggtgcc caccgcggtg ttcctgcagg acacgctgag
     5101 cggcccgaaa gatatcgcct ggggccagca gcttcgcgcc acgaaggacg aactcgagtc
     5161 catcggcctg aatctccgct gggatgtttc cgacagcttc gcgctggagt tcgacggtca
     5221 tacgtccgaa tccaagagcg atccgaacgg cccgctgggc cggacgtcct tcgatttcgg
     5281 cacgggcgcc gcggcaatca cggcgcacac gctcgatctc accacgggct ttccgattca
     5341 gcagttcacc tacaccgata ccaacggcaa caacaacggc atcatcgata tcgcggacgt
     5401 gagctcctcg gtgggacgca ccgcgatgca gagccaggtg cacgacatcg acgagttccg
     5461 gctcgatgcg gaattcacgt tcaacgactc gatgaagctg agtggtggtg tggactaccg
     5521 cacctccagc atgacgcagg agcggctcgt gaccgcgcag gtattgggcg actggggtgt
     5581 caccgcgcct ggcgacatcg agtcccaggc accgggcgtt ctcgaagcct attgcctgag
     5641 ctgcctgtat gacgacttct caccaggcct cggtgcgacc gcgttccgcg gcaacgccgc
     5701 ggacctgatg tccgcactgg ctccttacta cctcgatctc ggccatccga tccaggtctg
     5761 gaacaacgac cacaacagcg tggacgaaga catcacgtcc atctacgcgg agttcgcgtg
     5821 gaacggagag ctgggtggac ggaaggcgtc actggtcgcg ggtcttcgct acgaagagac
     5881 cgacgtcacc gcggtgagcg tgctcgccgt gcccgatggc gtgatctgga cggcggacaa
     5941 cgacttcgct tcggacttct cgagcggaac ggtgctgccg ctttccgacg atgcaagcta
     6001 tgacaatttc ctgccgaacc tcgacttctc ggtcgagctg ctggacaacg tcatcgcccg
     6061 tgcctcgtgg agcaagacca ttgcgcgcgc ggactacgga cagctgtttg cttccgagtc
     6121 cgccggtacg ccaccgcggg ctaccgcgct cggcggtatt gcaaccggtt cgagcaacaa
     6181 caccgcgctc gtgccactcg aatcgaccaa catcgacatc tcgctcgagg tgtactacgg
     6241 cgaggcgagt tacatctcga tcggattctt caacaaggat gtggagaatt tcatcggtac
     6301 gggacagatc actcgtccga tgttcgatct gcgcgacgtg agctcgggtg ctccgggaac
     6361 acggtcgggc gccgcatcga cggcgctggc cggaattccg ggcgcgctgc gtaacgacgt
     6421 caacatgttc gtgatgaccg cgatgatcga caatccgacg gcattcccgg atccgacggc
     6481 gacgttcctg gccaactcca cgaacggcga gttgaaccag gtcttcgcgg atcagatctt
     6541 cgctgcgtac aacctgcagt ccaatgcgag cgatcccttg ttccagtacc aggtatcccg
     6601 gcccatcaac cagaatgacg caaacattca tggtgtggaa ttcgccttcc agcatttctt
     6661 cggcgaatcg gggttcggca tccagggtaa ctacacgttc gtggatggcg acgtggccat
     6721 caacgtcgcg ggtgacccgg gcgtcgatca attcgcactg gaagggttga gcgacacggc
     6781 gaacgccacg ctgatgtacg agaagtacgg cttcacggct cgtatctcgt acaactggcg
     6841 cgacgaattc ctgaaccagg tcagccgcgg tggctaccgg aatccgacgt tcgtcaatgc
     6901 gttcgaggaa atcgacctga acttgagcta tgacatcaac gatgccctgg ctgtgagttt
     6961 cgaagccatc aacctcacgg gtgaagactt gcgcacccgc agtcgcacgg atgtggacta
     7021 ctggttcgcc caggagttgc acgcgcgcta cctgctggga gttcgctaca agttcaacta
     7081 gcctcgtgac gatacgaaag ggccgctgtt cacgcagcgg ccctttttac ttgccgctgc
     7141 ttcgaaagtt gttttagatg cgccatggcc gaaatcgctc gtaaaaccca ggtcatcacc
     7201 ggcgtcgcgc cggatcgcat tccctatgcc gagctgctcg aagcggggca gccgtccatt
     7261 ctcaaaggtg tggcgcgcga ctggtcgctg gtgcgcaatg cccgcgaatc tccggaaaag
     7321 gccatcgcct atctaaagaa attctgcggc gtcacgccca tcaccgtgtt cctggggcct
     7381 ccggaaatcg ccgggcggtt cttctacaac gagaaggtca ccggattcaa tttcgcggct
     7441 cacaaggtgc cgctggccga gacgctcgac cgcctgcagc tgctgatgag cgacgagcat
     7501 gctccggctg tctatgtcgg atccaccgac atcgacctct atctaccggg gctgcgcgcg
     7561 gaaaacgatc tgccgctcaa cgaccccatg ttcacgcaca accggccgct ggtgagcatc
     7621 tggttgggca accgcaccgt ggcgtcggcg catttcgact cgacccacaa tctcgcctgc
     7681 tgtgtggcgg gccaccggcg attcacattg ttccctcccg accaggtgca caacctgtac
     7741 cccggacctc tcgaaccgac accgggcggg caggtgatca ccatggtcga tttcaacgcg
     7801 cccgacttcg aacgctttcc gcggtttcgc gaggcgcttg ccgcggggca ggtcgccgac
     7861 ctcgaacccg gcgacgtgct gttctatccc gccatgtggt ggcatcacgt cgaagcgctg
     7921 gacccgttca acgcgctgat caactactgg tggacgacga cgccccgata catggactcg
     7981 ccgcaaacca cgttgatgca cgcactgctg agcctgcgag acctgcgtcc gcaggaaaaa
     8041 tccgcctggc gcgaactgtt cgactactac gtgtttggca ctgcgggacg ggccgccgaa
     8101 cacctgcccg agtccgcgcg cggcaatctc ggacccatgg acgacaccaa ggcgcggcgg
     8161 ctgcgcgcat ttctgctcag tcgcctgaat cgttgatcca cccgatgagg tcagtttgac
     8221 atcgcaatcc gtaaagaagg tggtcatcac cggtggcggc accgcgggct gggtgacagc
     8281 ggccgcgctc gcgcaccagt tttcgtcgct gctcgacatc acgctggtcg agtccgaaga
     8341 gataggaacg gtgggagtgg gtgaagccac gttcccctcc attctcgcgt tccatcgtct
     8401 gatcgaactc gatgagcaga aattcatgcg agcggccaaa gccagtttca agctgggcat
     8461 ctcgttcgag aactggggcg gtctcggcga tcgctacatc catgcgttcg gcaccgtcgg
     8521 caaatccacg tggatgggcg acttccagca tttctggctg gcggcgaggg aaggcggttt
     8581 tgccggggac ctcggcgaat actgcttcga acgtcaggcc gcactcgccg gcaagttcgc
     8641 gacgtccgaa gcggtcaaga tcaactacgc ctatcacttc gatgcggggc tgtacgccgc
     8701 ctatctacgt cagatcagtg agcccaaggg cgtcaggcgg atcgaaggca agatcaagag
     8761 tgtcgagcag catccggaaa gcggattcgt ccaggcgctc gttctcgaat cgggccagaa
     8821 ggtggaaggg gacctgttcg tcgattgctc cggattccgc ggcctgctga tcgagcagac
     8881 gctcaaggca ggatacgaag actacagtcc gtggctgcgc accgatcgcg cgttcgccgt
     8941 gcagacgcct tccggcaagg acatcccgcc gtacacgcgc gccatcgcgc ggcaggccgg
     9001 ctggcagtgg cgcattccgc tgcaacatcg cgtcggcaac ggcatggtct tcagcagcca
     9061 gtaccagagt gaagacgagg cgcgcgccac gttgttagcc aatctcgaag gcgaagcgct
     9121 gttcgagcca cgcctgctca agttcaaggc gggccggcga ctcaaggcgt gggacaagaa
     9181 cgtcgtgtcc atggggctcg cggggggatt catcgaaccg ctggaatcga ccagcattca
     9241 tctcatccag atcggcgtca cgcggctcat gcagctgttt ccgtttgccg gcattcaccc
     9301 cgcgctggtg aaccgataca acgaccagtc gcgcatcgaa tacgagcgaa tccgtgactt
     9361 catcatcctt cactacaaac tcaccgagcg cgacgacacc gcgttctggc gcgactgccg
     9421 cgacatgccg attcccgaca cgctggccga gcgcatcgag ctgttccgcg agagcgcgat
     9481 ggcttaccag gccacggacg agatgtttcg cgtcgactcc tggatgcagg tgatgatcgg
     9541 ccagcggctc atcccgcgcg gttaccacca catgggccgc atgctcgggc ccgaacgcct
     9601 gcgcaaggcg ctcgatacgc tcaagggcag tatctcgggg gcggtggcga gaatgcccac
     9661 ccaccgggag tacctgaact cctactgcgc cgcaggcgaa tgaggctccg gaataccccc
     9721 gaggcgggcc cccggcaggc ccatccgggg ctgccatata taagaggggg ctaaacccct
     9781 gatgcagcgc accaattcgt tgacaccccc cggtcacata cctagactcg ccggcctttg
     9841 tcgccccacc cctatcccgg ggaaggcgta aatagcggat ttgagaacac cgggccgttg
     9901 tcctaaacgg cccggtgttc attcgtccgc gtagtggatt aaaaactgga gtacgcatgg
     9961 ccacaacgag ccagttgatc gcgaacccgc gtcgtgtcaa caaagagaag accaaggtcc
    10021 ccgcgctcgg cgcgtcaccg cagaagcgcg gcgtttgcac gcgcgtgtac gcgacgacac
    10081 cgaagaagcc gaattcggcg atgcgcaaag tggcgcgtgt gcgtctcgtc aacggttacg
    10141 aagtctcttc gtacatcggc ggcgaaggcc acaacctcca ggagcactcg gtggtgctca
    10201 tccgcggcgg tcgcgtgaag gatctccccg gcgttcgtta tcacacggtg cgtggcacgc
    10261 tcgattgcgc cggcgttggc aatcgcaagc aaggccgctc caagtacggc gccaagcgcg
    10321 cgaagaaata agggttcaag tcatgtcacg tcgcacagca gcccccgtcc gccacatcct
    10381 gccggacccg aaattcaaca acgacatgct cgccaagttc atgaacgtcg tcatgaagag
    10441 cggcaagaag tcggccgccg aacgcatcct ctacggcgca atcgatcgca tcgtcgagaa
    10501 gtccggcaaa cccgccactg atgcggtcgc catgctttcg accgcgctcg acaacgtgaa
    10561 gcatgtggtc gaagtgaagt cgcgccgcgt cggcggcgcc acctaccagg tgcccgtcga
    10621 agtccgcgcg cagcgccgcc agacgctggc catgcgctgg gtcatcgaag ccgcgcgtgc
    10681 gcgcagcgag acgtcgatgg ctttccgcct tgcccatgag ctcatggacg ctgccgaaaa
    10741 ccgcggcgcc gccgttcgca agcgcgaaga cacccatcgc atggccgaag ccaacaaggc
    10801 attcgctcac taccgttggt aagccttcgg gccggtagat agctccatgc cccgcaatac
    10861 gcctatcgag cgctatagga acatcgggat ttccgcgcat atcgacgccg gaaagacgac
    10921 gaccaccgag cgcatcctgt tttacacggg cgtgtctcac aagatcggtg aggtgcacga
    10981 cggtgcggcg gtcatggact acatggagca ggagcaggag cgcgggatca cgatcacctc
    11041 cgcggccacc acctgcttct ggaaggggat ggacaagtcc ttccccgagc accgcatcaa
    11101 catcatcgat acgcccgggc acgtcgactt caccatcgaa gtcgagcgct cgctgcgcgt
    11161 gctcgattcg gcggtagcgc tgttcgattc ggttgccggt gtgcagccgc agtccgagac
    11221 ggtgtggcgg cagatgaacc gctacaacgt gccgcgcatc gccttcgtca acaagatgga
    11281 tcgcgccggc gcggatttcc tgcgcgtctg cgagatgatc cgcgcgcgcc tgcgcgccaa
    11341 tcccgtgccc atccagctgc ccattggcgc tgaagattcc ttcgaagggg tcgtcgacct
    11401 catcaagatg aaggccatct actgggacat ggaaacccag ggaatgaaat tcgagttccg
    11461 cgagattccg gcgccgctca aagcgcaggc cgaggaatac cgcgcgaaga tgatcgaagc
    11521 cgccgccgag ggcgacgacg cgctcaccga caaatacctg ggtggcggga cgctgtcgga
    11581 cgacgagatc aagcgtggcc tcaaggcgcg cgcgctcaag aacgaaatcg tgctctgcat
    11641 gtgcggcacc gcgttcaaga acaagggcgt gcaggcgctg ctcgatgccg tcatcgaatt
    11701 catgccgtcg cccatcgagc ttccgcccac caaggggctg tccgagaagg gcgagacgat
    11761 cacgcgaacc gcggaggacg gtcagccgtt ctcggcgctg gcattcaaga tcctcaacga
    11821 tcccttcgtc ggcaatctca cgttcttccg cgtgtattcc ggcacgctga attccggcga
    11881 cacggtgtac gtgccgtcgc gctcgaagaa agagcgcatc gggcgcctgc tgcagatgca
    11941 tgcgaacgag cgcagcgaga tcaaggaagt gcacgccggc gacatcgccg cggccgtggg
    12001 tctcaaggac gtctataccg gcgacacgct gtcggatctc gagaaggtca tcacgctcga
    12061 gaagatgacc attccgatcc ccgtcatctc ggtggcggtg gagccgaaga cgcaggccga
    12121 ccaggaaaag atgggcgtcg cgctgcagcg tctcgcgaaa gaagatccct cgttccgcgt
    12181 gtcgaccgat gaagagtccg cgcagaccat catcagcggc atgggcgaac tgcacctcga
    12241 gatcatcgtc gaccgcatgc gtcgcgagtt caaggtggaa gccaacgtcg gcaagccgca
    12301 ggtcgcgtat cgcgaaacga tccgcgccaa gatcgagagc gaaggcaaac acatcaagca
    12361 gtccggtggc cgcggccagt tcggacacgt gtggctcaag atcgaaccca acgaacaggg
    12421 caagggcaac gaattcgtca atggaattca gggcggcagc gtgccgcgtg agttcattcc
    12481 cgccgtcgag aagggcatca aggaagctct cgagaccggc gtgatggcgg gttaccccgt
    12541 cgtcgatacc aaggtcacgc tgttcgacgg ttcgtaccac gacgtcgact cggacgaaat
    12601 ttccttccgc atggccgcga tcttcggttt caaggacggc ttccgcaaag cgaagcccgt
    12661 gatcctcgag cccatcatga aggtcgaggt cgtgaccccc gaggattaca tgggcgacgt
    12721 gatcggcgac ttgaatcgcc gccgcggcca gatccagggc atggaagaca cgccggccgg
    12781 caagacggtc acggcggaag taccgctttc ggaaatgttc ggttacgcga ccaatgtgcg
    12841 ctccatgagc cagggccgcg cgaccttcac gatggaattc gccaagtaca tggaagttcc
    12901 gccgactatc gccgatggaa tcatcaacaa gtagtttcag aacgttctat taggagtccg
    12961 ggtcatgtcg aaggaaaagt tcgaacgcaa caaaccgcac gtcaacgtcg gcacgatcgg
    13021 tcacgtcgac cacggcaaga ccacgctgac ggctgcgctc acgaaggtga tggcggagac
    13081 caacggtggc tcgtacatgg cgtacgacca gatc
//
LOCUS       DATDEU010000110         3458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1188094, whole genome shotgun sequence.
ACCESSION   DATDEU010000110 DATDEU010000000
VERSION     DATDEU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188094"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VIV63_02665"
     CDS             complement(<1..447)
                     /locus_tag="VIV63_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6123528.1"
                     /translation="MSKEKFERSKPHVNVGTIGHVDHGKTTLTAALTKVMAQLHGGEF
                     KAYDQIDKAPEEKARGITISTAHVEYTSDNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYMNKADMVEDKELLEL"
     gene            complement(476..2563)
                     /gene="fusA"
                     /locus_tag="VIV63_02670"
     CDS             complement(476..2563)
                     /gene="fusA"
                     /locus_tag="VIV63_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY6123529.1"
                     /translation="MPRVTPLERYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAAVMDYMEQEQERGITITSAATTCFWKGMENIFPEHRINIIDTPGHVDFTIEVERS
                     LRVLDSAVALFDSVAGVQPQSETVWRQMNRYGVPRIAFVNKMDRAGANFLRVIDMIRE
                     RLRANPIAIQLPIGAEDDFQGVVDLIKMKAIYWDMETQGMKFEYREIPANLQAQAEEY
                     RAKMIEAAAEGNDALTEKYLNGQELTSDEIKQGLKSRSLKNELVICMCGTAFKNKGVQ
                     ALLDAVIEFMPSPMELPDTKGLDEDGKPATRKPDDAAPFAALAFKILNDPFVGNLTFF
                     RVYSGVMNTGDAVAVPGRGKRERIGRLLQMHASERTDIKEVRAGDIAAAVGLKDVNTG
                     DTLCSESKIITLEKMVIPIPVIAVAVEPRTKVDQDKMGMALQRLAKEDPSFRVSTDEE
                     SGQTIISGMGELHLEIIVDRMKREFKVEANVGKPQVAYRETIRAKVESEGKHVKQSGG
                     RGQYGHVWLKLEPNEEGKGIEFVNGIVGGAVPREFIPAIEKGVKEAAETGVIAGYPVV
                     DVKVTLFDGSFHDVDSDEISFRMAAIFGFKDGFRAAKPVILEPIMKVEVVTPEEYMGD
                     VIGDLNRRRGQIHGMEDTPAGKTVMAEVPLAEMFGYATNVRSMSQGRATFTMDFAKYM
                     EVPANVADTIIKQ"
     gene            complement(2625..3104)
                     /gene="rpsG"
                     /locus_tag="VIV63_02675"
     CDS             complement(2625..3104)
                     /gene="rpsG"
                     /locus_tag="VIV63_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY6123530.1"
                     /translation="MSRRAAAPTRHILPDPKFNSDMLAKFMNVVMQDGKKSAAEQILY
                     GAIERIGEKTGKQGPEAIELLSLALDNVKPAVEVKSRRVGGATYQVPVEVRSSRRQTL
                     AMRWVIDAARARTEKSMAYRIAHELMDAAENRGGAVRKREDTHRMADANRAFAHYRW"
     gene            complement(3123..>3458)
                     /gene="rpsL"
                     /locus_tag="VIV63_02680"
     CDS             complement(3123..>3458)
                     /gene="rpsL"
                     /locus_tag="VIV63_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY6123531.1"
                     /translation="VNKEKTKVPALGASPQKRGVCTRVYATTPKKPNSAMRKVARVRL
                     VNGYEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSLDCSGVNDRRQSRS
                     KYGAKRPKK"
BASE COUNT          539 a         1090 c         1079 g          750 t
ORIGIN      
        1 gagctccagc agctccttgt cctccaccat gtcggccttg ttcatgtaca ccacgatgta
       61 cggaacgccc acctggcggg ccagcaggat gtgctcgcgc gtctgcggca tcgggccgtc
      121 tgcagccgac accaccagga tcgcgccgtc catctgcgcg gctcccgtga tcatgttctt
      181 cacgtagtcg gcgtgcccag ggcagtccac atgtgcgtag tggcggttgt ccgacgtgta
      241 ttccacgtgc gccgtcgaga tcgtgatgcc gcgcgccttc tcttcaggcg ccttgtcgat
      301 ctggtcgtac gccttgaact cgccgccatg cagctgcgcc atcaccttcg tcagcgcggc
      361 cgtcagcgtc gtcttgccat ggtcaacgtg accaatcgtg cctacgttga cgtggggttt
      421 gctgcgctcg aatttttcct tcgacatgtg tgtgttcctg gcttaagtgt gactgttact
      481 gtttgatgat ggtgtcggcg acatttgccg gcacttccat gtacttcgcg aagtccatcg
      541 tgaacgtggc gcgcccctgg ctcatcgagc gcacgttggt ggcatagccg aacatctccg
      601 cgagcggaac ctcggccatg accgtcttgc ctgcaggcgt gtcttccatg ccatggatct
      661 ggccacgacg acggttgagg tcgccgatga catcgcccat gtattcctcg ggcgtcacga
      721 cttcgacctt catgatcggc tcgaggatca ccggcttcgc cgcgcggaag ccatccttga
      781 agccgaagat cgccgccatg cggaacgaga tttcgtcgga gtccacgtcg tggaaggatc
      841 cgtcgaagag agtgactttt acatcaacga ctggatagcc agcgatgacg ccggtttccg
      901 ccgcttcctt gacgcccttc tcgatcgccg gaatgaactc gcgcggcacg gcgccgccga
      961 cgatgccatt cacgaattcg atgcccttgc cttcttcgtt cggctcgagc ttcagccaca
     1021 catggccata ctggccacgg ccgccggatt gcttgacgtg cttgccctcg gactcgacct
     1081 tggcacggat ggtttcgcgg taggccacct gtggcttgcc cacgttggcc tcgaccttga
     1141 actcgcgctt catgcggtcg acgatgatct cgaggtgcag ctcgcccatg ccggaaatga
     1201 tggtctggcc cgactcttcg tcagtgctga cacggaagga agggtcttcc ttcgccagcc
     1261 gctgcagcgc catgcccatc ttgtcctggt cgaccttggt gcgcggttcc acggccacgg
     1321 cgatgaccgg gatcggaatg accatcttct cgagcgtgat gatcttgctc tcgctgcaga
     1381 gcgtgtcgcc ggtgttgacg tctttcaggc ccacggctgc ggcgatgtcg ccggcgcgga
     1441 cttccttgat gtcggtgcgc tcactggcat gcatctgcag caggcggccg atgcgctcgc
     1501 gcttgccgcg accgggcact gccacggcgt cgccggtgtt catcacaccc gaatagacac
     1561 ggaagaacgt gaggtttccg acgaacggat cgttgaggat cttgaacgcg agcgcggcga
     1621 acggcgcggc gtcgtcgggc ttgcgcgtgg ccggcttgcc gtcctcgtcg aggcctttcg
     1681 tatccggcag ctccatcggc gacggcatga attcgatcac ggcgtcgagc agcgcctgca
     1741 cgcccttgtt cttgaacgcg gtgccgcaca tgcagatcac gagctcgttc ttcagcgagc
     1801 ggctcttgag cccctgcttg atctcgtcgg aggtgagttc ctggccgttg agatatttct
     1861 cggtgagcgc atcgttgccc tcggcggcgg cctcgatcat cttcgcgcgg tattcctccg
     1921 cctgcgcctg caggttcgcc gggatctcgc ggtattcgaa cttcatcccc tgggtttcca
     1981 tgtcccagta gatcgccttc atcttgatga ggtcgaccac gccctggaaa tcgtcctcgg
     2041 cgccgatcgg cagctggatc gcgatcgggt tcgcgcgcag ccgctcgcgg atcatgtcga
     2101 tcacgcgcag gaaattggcg ccggcacgat ccatcttgtt gacgaaggcg atgcgcggca
     2161 cgccgtagcg gttcatctgc cgccacaccg tctcggactg cggctgcacg cccgctaccg
     2221 aatcgaacag cgcgacggcg ctgtcgagca cgcgcaggct gcgctcgact tcgatcgtga
     2281 agtccacgtg gcccggggta tcgatgatgt tgatgcggtg ttcggggaag atgttctcca
     2341 tccccttcca gaagcaggtg gtcgcggcgg aggtgatcgt gatgccacgc tcctgctcct
     2401 gctccatgta gtccatcacg gctgcgccgt catggacctc gccgatcttg tgcgaaacgc
     2461 ccgtgtagaa aaggatgcgt tcggtcgtgg tcgtcttgcc cgcgtcgatg tgcgcggaga
     2521 ttccgatgtt ccgataacgc tcgagtggcg taacgcgtgg catgttgaat gtatagcgct
     2581 tcgcgcgggt tcaacggagc ccgcgagcgg gctccgtacg acagttacca gcgatagtgc
     2641 gcgaaggcgc ggttggcgtc ggccatgcga tgcgtgtctt cacgcttgcg cacggcgccg
     2701 ccgcgattct cggctgcatc catcagttcg tgggcgatgc ggtaggccat cgacttctcg
     2761 gtgcgcgcgc gggcggcatc gatgacccag cgcatggcca gggtctggcg acgcgacgag
     2821 cgcacttcga cgggcacctg gtaggtggca ccaccgacgc ggcgcgactt cacctcgacc
     2881 gcaggcttca cgttgtcgag cgccagcgac agcagttcga tggcctcggg gccctgcttg
     2941 ccggtcttct cgccgatgcg ctcgatggca ccgtagagga tctgttcagc ggcggacttc
     3001 ttgccgtcct gcatgaccac gttcatgaac ttggccagca tatcgctgtt gaatttcgga
     3061 tccggaagga tgtggcgggt aggtgctgct gcgcgacggg acattgcaaa aactcctgga
     3121 gactatttct tcgggcgctt ggcgccgtac ttggaacgac tctggcgacg atcattcacg
     3181 ccggagcagt cgagcgaacc acggacggtg tgataacgca caccggggag atccttgacg
     3241 cggccgccgc ggatgagcac caccgagtgc tcctgcaggt tgtggccttc gccgccgatg
     3301 tacgaagaga cttcgtaacc gttgacgagg cgcacacgcg ccactttgcg catcgccgag
     3361 ttcggctttt tcggcgtcgt cgcgtacacg cgcgtgcaaa cgccgcgctt ctgcggtgac
     3421 gcgccgagcg cggggacctt ggtcttctct ttgttgac
//
LOCUS       DATDEU010000111         2966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_459221, whole genome shotgun sequence.
ACCESSION   DATDEU010000111 DATDEU010000000
VERSION     DATDEU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459221"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VIV63_02685"
     CDS             complement(<1..453)
                     /locus_tag="VIV63_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY6123532.1"
                     /translation="MPKTTSLAVVMDPIAKIKFAKDSTLAMLLAAASRGWQLTYFEQS
                     DLFLRDGIAFGRGRDLRVFADPAKWFELGEPSVRQLGDFDCVFMRKDPPFDAEYIYTT
                     YILERAEIQGAFVANRPQGLRDMNEKVFTAWFPQCCAPTLITRSAADIT"
     gene            628..993
                     /gene="pilG"
                     /locus_tag="VIV63_02690"
     CDS             628..993
                     /gene="pilG"
                     /locus_tag="VIV63_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HEY6123533.1"
                     /translation="MKVLVIDDSKTIRKTAETLLAKEGCEVFTAVDGFDALAKVADYT
                     PDIVFVDIMMPRLDGYQTCSLIKHNKVFKSIPVIMLSSKDGLFDRARGRIVGSEYYLT
                     KPFTKDELLSAIETHVAAR"
     gene            996..1361
                     /locus_tag="VIV63_02695"
     CDS             996..1361
                     /locus_tag="VIV63_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6123534.1"
                     /translation="MALILIVDDSPTEVHVMKKALEKSGYQTATAQDGAEGVRMAREM
                     TPDLIFMDVVMPGVNGYQATRTLANDPKTKTIPIIMVTSKGQETDKIWGLRQGAVDYM
                     VKPVSPDQLVAKAQATLAA"
     gene            1372..1935
                     /locus_tag="VIV63_02700"
     CDS             1372..1935
                     /locus_tag="VIV63_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY6123535.1"
                     /translation="MNEPATLKSLRDRPFELLAELERRGRAVTAAAASENPGAREWVG
                     VAFRMAGELYLAAREETREVLAVPAGLTRVPGAKPWVKGIANVRGQLLPILDLRQYLG
                     SGVTPNTRNVRVIVVNHREVPAGLVVDEVLGFRRFSESEFAGDAPPTVVRCERYLAGA
                     FRRGPEQWPVLSLRQLVESPSFQEAAA"
     gene            1932..>2966
                     /locus_tag="VIV63_02705"
     CDS             1932..>2966
                     /locus_tag="VIV63_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6123536.1"
                     /translation="MSTEVGGGLRTLKSINSLITASLVCVALGVLVGVAAFLISLDLL
                     YAVAAAFAVAALIPLFLMFGRVRAVEADIGHREALSLAQRKETERNQQAILRLLDELA
                     PLADGDLTVQATVTEDITGTIADSINYAIGVLRDLVSTINQSAIQLDGATRQTQALSA
                     HLAKASSAQSKQIAGATESASNMASSTEAISGNSERASDVARHSVDVAHKGGDAVRRT
                     IDGMNAIRETIQETSKRIKRLGESSQEIGNIVELINDIAEQTNILALNASIQASMAGD
                     AGRGFAVVADEVQRLAERAAAATRQIETLVRAIQADTNEAVVSMERSTTDVVGGALLA
                     ENAGAALEEIE"
BASE COUNT          607 a          947 c          913 g          499 t
ORIGIN      
        1 ggtgatgtct gccgcgctac gcgtgatcag cgtcggcgca cagcactggg gaaaccaggc
       61 ggtgaagact ttctcgttca tgtcgcgcag gccctgcggc cggttggcga cgaacgcgcc
      121 ctggatctcc gcgcgctcga gaatgtaggt ggtatagatg tactcggcgt cgaacggcgg
      181 atccttgcgc atgaacacgc agtcgaagtc gccgagctgt cgaaccgaag gctcaccgag
      241 ctcaaaccac ttcgccggat ccgcgaacac ccgcaaatcc cggccacgcc cgaaagcgat
      301 gccatcacgc agaaacaggt ccgactgctc gaaataggtg agttgccagc cacgagaagc
      361 cgccgcaagc aacatcgcaa gtgtggagtc cttcgcaaat ttgatcttcg cgatcggatc
      421 catcacgacg gcaagactag tggttttcgg catcgcgaac tccgaactca aaaacgtgac
      481 gtgaccggca tagccggggg tatgcctata tgttaaaacg cccctcagct tgccaagtgg
      541 cgtcgcattt taatagcggc gttgaaggcc attttgtttg ttcaggagag gtacgcggtg
      601 acagatacca acccacacct caaaggtctg aaagtcctcg ttatcgatga cagcaagacc
      661 attcggaaga cggcggaaac gttgctcgcg aaagaaggct gcgaagtctt cacggcggtc
      721 gacggcttcg acgcgcttgc gaaggtggcc gactacacac ccgacatcgt cttcgtggac
      781 atcatgatgc cgcggctcga cggctaccaa acctgttcgc tcatcaaaca caacaaagtg
      841 ttcaaatcga ttccggtgat catgctgtcg tcgaaagacg ggctgttcga tcgcgctcgt
      901 ggacgcatcg tgggatcgga gtactacctc acgaagccat tcacgaagga cgaactgctc
      961 tctgcgatcg agacgcacgt cgcggcgagg tgagtatggc tctcattttg atcgtggacg
     1021 attccccgac cgaagtgcac gtcatgaaga aggcgttgga gaagtctggc taccagacgg
     1081 ccaccgccca ggacggcgcc gaaggcgtgc gcatggcgcg cgagatgact cccgatctca
     1141 tcttcatgga cgtcgtcatg ccgggagtga acggctacca ggcgacgcga acactggcca
     1201 acgatccgaa gaccaagacc attcccatca tcatggtcac ctcgaaaggc caggagaccg
     1261 acaagatctg gggactgcgc cagggcgcgg tcgactacat ggtcaaaccg gtttcgcccg
     1321 atcaactcgt ggcgaaggcg caggcgaccc tcgctgcctg agcctcccga aatgaacgag
     1381 cccgccaccc tcaaatcgct gcgtgatcgc cccttcgaac tcttagccga gctcgaacgt
     1441 cgcggccgcg cggttaccgc cgccgccgct tcggaaaatc cgggtgcgcg cgaatgggtg
     1501 ggcgtggctt tccgcatggc cggagagctc tatctcgcgg cgcgcgaaga aacccgcgaa
     1561 gtgctcgcag tgcctgccgg tctcacccgg gtgccaggcg ccaagccgtg ggtcaaaggc
     1621 atcgccaacg tgcgcggcca gctcctgcct atcctcgacc tgcgtcagta cctgggctcg
     1681 ggtgtcacgc cgaacacgcg caacgtgcgc gtcatcgtcg tcaaccaccg tgaagtgccc
     1741 gcgggtctcg tggtcgacga agtgctcggc ttccgccggt tctccgaatc cgaatttgcc
     1801 ggcgacgcgc ctccgacggt ggtgcgttgc gaacgatatc tggcgggcgc gttccggcgc
     1861 ggtcccgaac aatggccggt gttgagcctg cggcagctgg tcgaaagtcc gtccttccag
     1921 gaggcggcgg catgagcacc gaagtcggcg gcggtttgcg cacgctgaaa tccatcaact
     1981 cactcatcac cgcctcgctg gtctgcgtgg cgctcggagt gctggtggga gtggctgcct
     2041 ttctgatcag cctcgacctg ctctacgcgg tggctgccgc attcgccgtg gccgcgctca
     2101 taccgctgtt cctcatgttc ggacgcgtgc gcgcggtcga agccgacatc gggcatcgcg
     2161 aagcgttgtc gctcgcgcag cgcaaggaaa ccgaacgcaa ccagcaggcc attctccggt
     2221 tgctcgacga actcgcgccg ctcgcggatg gcgacctcac cgtccaggcg acggtgaccg
     2281 aagacatcac gggcaccatc gccgattcca tcaactacgc catcggcgtg ttgcgcgacc
     2341 tggtgagcac catcaaccag tcggccatcc agctcgacgg cgccacgcgg caaacgcagg
     2401 ctttgtcggc gcacctggca aaggcgtcga gcgcgcagtc caagcagatc gccggcgcga
     2461 ccgaatccgc cagcaacatg gcgagcagca ccgaagcgat ctcgggcaac tccgagcggg
     2521 cgtcggacgt cgcgcgccat tcggtcgatg tcgcccacaa gggtggcgac gcagtgcgcc
     2581 gcacgatcga cggcatgaac gccatccgcg aaaccattca ggaaaccagc aagcgcatca
     2641 agcggctggg tgagagctcg caggaaatcg gcaacatcgt cgaactcatc aacgacattg
     2701 ccgagcagac gaacattctc gcgttgaacg cgtcgatcca ggcatcgatg gccggtgacg
     2761 cgggccgcgg cttcgcagtg gtcgccgacg aagtgcagcg cctcgccgag cgcgccgccg
     2821 ccgccactcg ccagatcgaa acgctggtgc gcgccattca ggcggatacg aacgaagccg
     2881 tcgtctccat ggaacgcagc accacggacg tcgtgggcgg cgcattgctc gccgagaacg
     2941 ccggcgcggc actcgaagag atcgag
//
LOCUS       DATDEU010000112        10334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1334394, whole genome shotgun sequence.
ACCESSION   DATDEU010000112 DATDEU010000000
VERSION     DATDEU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334394"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VIV63_02710"
     CDS             <1..450
                     /locus_tag="VIV63_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HEY6123537.1"
                     /translation="DFKNHDPVLRDILKVHRENPACASCHNRMDPIGLAFENFNALGM
                     WRDTERKQPIAAQGNLITGETFNSVAELKRILAQDHRQEFYATLSRKLLTYASGRGTE
                     YYDVETIDGIVKRLNANDGRFSALLMGVIESAPFQKMRVQATATVAN"
     gene            452..1768
                     /locus_tag="VIV63_02715"
     CDS             452..1768
                     /locus_tag="VIV63_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY6123538.1"
                     /translation="MNPFSRRHFFRGLGACIALPAFTSLAHGASRSAPTRMAVVYVPN
                     GAIPAAFWPAGEPGGAFALSPTLAPLANVHQQLQVISGLNDLSAEAGPDGAGDHARAS
                     GTFLTGVRIKKTAGSDIRAGVSIDQVVANQIGHLTRFSSLELTCDAVRKSGDCDSGYA
                     CAYEYNLAWRSHNQPLSPEHNPRFVFERLFGTGSPRERVGNLKRRQAEQKSILDFVLD
                     DARSVNAKLDGRDKQKLDQYLTSVREIETRIENSTRLSVKNPDIDAPAGIPDSYAEHV
                     ALMFDMLALAFQTDSTRVATLLISREGSNRSFADIGIASGHHDLTHHKNSPEIIEKVK
                     LIDRWYMQRFAQFLEKMQNTEDVDGKSLLDHSMILYGSGNADGNRHTHENLPILLAGG
                     GSGALKPGRYVKAANVPITNLFLTMADAMGAKGVESHGDSTGRLTV"
     gene            complement(1774..2700)
                     /locus_tag="VIV63_02720"
     CDS             complement(1774..2700)
                     /locus_tag="VIV63_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6123539.1"
                     /translation="MLNKTNLARIDLNLLVLFEAVLEEQHVGRAALRLHISPSAVSHG
                     LGRLRRLMHDPLFLRQPKGVVPTDRARLLAAPVADILERTRQVMTNAEEFDPQKSRRR
                     FLIGAPDAASAVLLPPLLAEMRTAAPGIDLGVRNLVGQFDLALGELDRRTLDVALLPL
                     ANVPARFVARTLYNEDFVIARRAGHPMGRKLTLARYCAAPHLVVSVSGDPHGMVDTYL
                     AERGLKRRVALTVSNFMQALAIVAESDLVAALPRQFVARHAARFKVVCSEPPAPLMSG
                     PIRAIAPQVATTDGGLSWLLGALERSAKVALR"
     gene            2793..3443
                     /locus_tag="VIV63_02725"
     CDS             2793..3443
                     /locus_tag="VIV63_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY6123540.1"
                     /translation="MTVLIITTLLAVGFLSARAFGHAVFRPTAIAGEHLPVTDLQALA
                     VVTFWIDAGPPLWFAKDVKFDTRFRERFLREHEAAARGELQHWLSTADGALALVILLD
                     QFPRNAFRGTRRMYDTDGLAREAARTAFAAGYDQQIAVELRKFFVLPFAHSEDMADQD
                     RSVALARRIGADDLGHAEHHRDIVRRFGRFPHRNAILGRDSTPAEQQYLANGGYAG"
     gene            complement(3587..4009)
                     /locus_tag="VIV63_02730"
     CDS             complement(3587..4009)
                     /locus_tag="VIV63_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123541.1"
                     /translation="MSQSGDTSTTQMSQRALSLRFLTRAQAEVAQMRACLPDEPMLIE
                     PAAVAHIERMAHHISSAAEAFGFAEIDAIAAAIELMSQPGPRLSVHERRALAAHLSEK
                     IAALSIYVEYELAEADAKRVPEELPISASLPGFGARRK"
     gene            4406..5593
                     /locus_tag="VIV63_02735"
     CDS             4406..5593
                     /locus_tag="VIV63_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY6123542.1"
                     /translation="MTTKPDETGIQIDVAAVQKILSVPTILDVVCRTTGMGFAAIARV
                     TDDRWIACSVRDEIGFGLAAGGELNIETTICNEIRQHREPVVIEHVAEDRKFFNHPTP
                     ALYGFQSYISMPIILPGGEFFGTLCAIDPKPARVNTPAIVNTFRLFAELIAFHLDADR
                     RLAVSEAALLNERETARLREQFIAVLGHDLRNPLGSISSAAHVLARTPLSEPAAKLVE
                     MMQRSVRRMAGLIDDVLDFARGRLGGGLALSLTGDGSLAPVLEQVIAELRVSFPDRVI
                     ETDFTPQDCVKCDRPRLSQLLSNLVANALTYSAPDTPVRASSRIVDGQFELTVTNQGE
                     PISPQIMERLFSPYERGAVRPSQQGLGLGLYIASEIARAHGGTLTVTSSPEETCFTLT
                     IPT"
     gene            5604..6116
                     /locus_tag="VIV63_02740"
     CDS             5604..6116
                     /locus_tag="VIV63_02740"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEY6123543.1"
                     /translation="MYTEMFAQMKKQLGQLDKWLGTASEYAKAKPFDPNVFVGQRLAP
                     DQFPLARQIQICCDTAKLAASFLTGKAAESQPDTEQTLEELQTRVRSIITYLDGISPQ
                     DLEGAATRVVSQPRWKGEWMTGADYFVQHAIPNFYFHLTTAYAILRHNGVSVGKRDFL
                     GAQTKRPPAN"
     gene            6177..7694
                     /locus_tag="VIV63_02745"
     CDS             6177..7694
                     /locus_tag="VIV63_02745"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY6123544.1"
                     /translation="MKPALAIRAYCLVGSVMLAACGGGGSVSGSSSSSSSSSSSSSSG
                     GSTPPPAITFNNASVHDPSIIKVGSEYLVFGSHLAAAKSTDLMNWTLIADGVTNTNPL
                     FTNVVTALQQTFTWSQVTDLWAPDVVRLADNKFYFYYNSCRGDAPLSALGVAVADSVV
                     GPYVNKQQFLRSGMTGLSDDGITSYDPLVHPNVVDPHTFFDATGNLWMIYGSYSGGIY
                     ILAMDEITGLPEAGQGYGTHLMGGNHARIEGAYVLYNAQTGFYYLFTSFGGLAANGMY
                     NIRVARSQAPDGPYFDAAGTDMATVTGNPAAAFDDTRIAPHGQKLMGNHQFALATGES
                     GTALGYVSPGHNSAYFDAGLAKYFLIFHTRFPNRGEQHEIRVHELLFNSQGWPVAAPF
                     RYAPLSLSPTAVVAEVTNADVAGAYKLIDHGKDISATIKLSQAIRLNADGTVSGGVTG
                     TWIHRGGNLIDLTTGGSLYYGVLSRQWNTNANRFVVTFSAQSVAGVSLWGARTGD"
     gene            complement(7717..8535)
                     /locus_tag="VIV63_02750"
     CDS             complement(7717..8535)
                     /locus_tag="VIV63_02750"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HEY6123545.1"
                     /translation="MRRSFFAGLAIALVSQSIVSQAEAAPARLPFRSSFETGTFSEWN
                     GGTTGRLVVTTEDASQGTHSVRGTVIAGTGADQYKDFYFGNHPTVGGEAVSVDRGLWL
                     SFDSKFDNGFVFGSNPVHKIMLVNFDDENARRRYQIIVNIWTPTGEYFLEHVVWNADR
                     SFNKTIPGIDQNVPPDAIMRRGVWEHIKLFIKPNTPGQSNGIVRMWVNGALKTDRTSV
                     AVRENTNFLPNKLIVGNYVTDLVTNGTQRWDNVVLSDVDPDGVRPSAPTNTTAQ"
     gene            complement(8711..>10334)
                     /locus_tag="VIV63_02755"
     CDS             complement(8711..>10334)
                     /locus_tag="VIV63_02755"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123546.1"
                     /translation="MVYDFDGDGRAEVMCKTADGTRSGTNQVIGSSTADHRNSSGYIL
                     AGPEFLTVFNGQTGAVLATTNYVPARGNVGDWGDTYGNRVDRFLAGVAYLDGQRPSAI
                     FSRGYYTRAVIAAWDFRNGQLTQRWVFDSNGSGNSAYAGQGNHNLSIADVDQDGRHEV
                     IFGAATIDDNGARMYTSGLGHGDALHVGDFIPSRAGLEIWDIHESSNQPGADLRDART
                     GARIFATPNNNGVEGPGRGVAADIWSGNEGAEYWGAGPNMTFLRNASGGNIGRNPGSA
                     NHLAWWDADTVRELVDGNHVDKYGTGGDTRLLTADGTTTSNGTKSNPALSGDLLGDWR
                     EEVVWRTTNNGALRIYTTTNVASNRIYTLLHDPQYRVAIAWQNTAYNQPPHPGFFIGA
                     NMPTPPTPNITLVGGTPPDPQPGTPTYQFETASLGGGTVSESTNSGFIGSGYANFSAT
                     GGFAQVNNADGRGGGSHAIRIRYALGTTASRTGRVLVNGVAQNITFNPTGAWTTWATQ
                     NVTFTLNTGTVNTIRFESNGQDLANLDQVEIL"
BASE COUNT         1851 a         3440 c         3193 g         1850 t
ORIGIN      
        1 gacttcaaga accatgaccc cgtgctgcgg gacatcctca aggtgcaccg cgaaaacccg
       61 gcctgcgcgt cctgtcacaa ccgcatggac cccatcgggc tcgcgttcga gaacttcaac
      121 gcgctcggca tgtggcgcga caccgagcgc aaacaaccca ttgccgcgca gggcaacctg
      181 atcaccggtg aaaccttcaa cagcgtggct gaactcaagc gtatcctcgc gcaggaccac
      241 cgccaggagt tctacgccac gctgtcgcgc aaactcctca cctacgcctc gggcagaggc
      301 acggagtact acgacgtgga gaccatcgac gggatcgtga agcggctcaa cgccaacgac
      361 ggccgtttct ccgcattgtt gatgggtgtc atcgaatccg cgccattcca gaagatgcga
      421 gtccaggcca cagccacggt agccaactga catgaatcca ttcagccgca gacatttctt
      481 ccggggactc ggcgcctgca tcgccctgcc cgcgttcact tcactggcgc acggcgccag
      541 ccgctccgcg cccacgcgca tggcggtggt gtacgtaccc aacggcgcca ttccggcggc
      601 gttctggccc gcgggtgaac ccggcggcgc attcgcgctg tcgcccacgc tcgcgcctct
      661 cgccaacgtg catcagcagc tgcaggtcat ttccgggctc aacgacctct ccgccgaggc
      721 cggtcccgat ggcgccggtg accatgcgcg cgccagcggc acgttcctca ctggcgtgcg
      781 catcaagaaa accgcgggct cggatatccg cgccggcgtt tccatcgacc aggtggtggc
      841 gaatcagatc ggccatctca cgcgcttctc gtcgctcgaa ctcacctgcg acgcggtgcg
      901 caagtcgggc gactgcgatt ccggttacgc ctgcgcctac gagtacaacc tcgcctggcg
      961 ttcgcacaac cagccgttgt cgcccgagca caatccgcgg ttcgtgttcg aacgcctgtt
     1021 cggcaccggc agcccgcgcg agcgcgtcgg caatctcaag cgacgccagg ccgagcagaa
     1081 atccattctc gacttcgtgc tcgacgacgc acgttcggtc aacgcgaagc tcgatggccg
     1141 cgacaagcag aagctcgacc agtacctgac gagcgtgcgc gagatcgaaa cccgcatcga
     1201 aaattccacg cgcctttcag tcaagaaccc ggacatcgac gcgccggcgg gcatccccga
     1261 cagctacgcc gaacacgtcg cgctcatgtt cgacatgctg gcgctcgcgt tccagaccga
     1321 ctccacgcgc gtcgccacgc tgctcatctc gcgtgaaggc agcaaccgca gcttcgccga
     1381 catcggcatc gcgtccggac accacgacct cacgcaccac aagaacagcc ccgagatcat
     1441 cgagaaggtg aagctcatcg atcgctggta catgcagcgg ttcgcgcagt tcctggagaa
     1501 gatgcagaac accgaagacg tcgacggcaa gtcgctgctc gaccactcga tgatcctcta
     1561 cggatcgggc aatgccgatg gcaatcgcca cacgcatgag aacctgccga tcctgctggc
     1621 cggcggtggc agcggcgcgc tcaagcccgg acgctatgtg aaggcggcga atgtgccgat
     1681 caccaacctc ttcctcacca tggcggacgc catgggagcg aaaggcgtcg agagccacgg
     1741 cgattccacc gggcgcctga cagtctgagt gtcctagcgc agcgcgacct tggcgctgcg
     1801 ctcgagtgca cctaacaacc agctgagacc gccatcggtc gtcgccacct gcggtgcgat
     1861 ggcgcggatc gggccgctca tgagcggcgc cggcggctcg gaacaaacca ccttgaatcg
     1921 cgcggcgtgc cttgccacga actgccgcgg caaggcggcc acgaggtccg actcggcgac
     1981 gatggccagc gcctgcatga agttcgacac cgtgagcgct acgcgccgct tcaggcctcg
     2041 ttcggccagg tacgtgtcga ccatgccgtg cggatcaccc gacacggaca ccacgagatg
     2101 gggcgccgcg cagtagcgag ccagcgtgag tttcctgccc atggggtggc cggcccgccg
     2161 ggcgattacg aaatcctcgt tgtacaacgt acgcgcgacg aaccgcgccg gcacgtttgc
     2221 cagcggcaac agcgccacgt ccagcgttcg ccggtctagt tcgccgagcg cgaggtcgaa
     2281 ctgaccgacg agattgcgca cacccagatc gatgccgggc gccgcggtcc gcatctcggc
     2341 gagcagtggc ggcaacaaca cggcgctcgc cgcatcgggc gcgccgatga ggaaccgccg
     2401 ccgggatttt tggggatcga actcttcggc gttcgtcatc acctgccgtg tgcgctcgag
     2461 aatatccgcg accggcgcag cgagcagacg cgccctgtcc gtgggcacca cacccttcgg
     2521 ctggcgcagg aacagcgggt cgtgcatgag gcgccgcagc cgcccgagac cgtggctgac
     2581 cgctgacgga gagatgtgca ggcgaagcgc cgcgcggccc acgtgttgct cttcgagcac
     2641 ggcttcgaac agcaccagca ggttgaggtc gatgcgcgcg agattagtct tgttcagcat
     2701 atgtatgaaa tcgtatcact ggattcagca tgagtccggc gcgattctgc gcgccggagg
     2761 aacccatgca cgatcgaacc cgtttattcg caatgaccgt tctcatcatc accacgctgc
     2821 tggccgtcgg cttcctgagc gccagggcgt tcggtcacgc ggtgttcaga cccaccgcca
     2881 tcgccggcga acacctgccg gtgaccgacc ttcaggcact ggccgtggtg actttctgga
     2941 tcgacgccgg cccgccactc tggttcgcca aggacgtaaa attcgatacg cgttttcgcg
     3001 aacgcttcct gcgtgaacac gaggcggcag cgcgcggcga actgcaacac tggctgagca
     3061 ccgccgacgg cgcgctggcg ctggtgatcc tgctggatca gttcccgcgc aatgcctttc
     3121 gcggaacgcg gcgcatgtac gacaccgatg gcctcgcccg ggaggctgcg cgcactgcat
     3181 tcgcggccgg ctacgaccag cagatcgcgg tcgaattgcg caagttcttc gtgctgccgt
     3241 tcgcacactc ggaggacatg gccgatcagg accggtcggt ggcgctcgca cggcgcattg
     3301 gcgccgacga cctcggccat gccgaacacc atcgcgatat cgtgagacgc ttcgggcgct
     3361 ttccgcatcg caacgcgatc ctgggccgcg acagcacgcc ggccgagcag cagtacctcg
     3421 ccaatggcgg gtatgccgga tgaacgcgaa ggaaatgcca gccgatcagt tcgacgatga
     3481 acgattagcg ctggcgtggc tgcgcctggc ggtgcgaagc gctacgtgcg gcgcggcttc
     3541 cgcgacgcgg gtgatgatcc tggacccggt gaggtgccct gcggcgttat ttccgccggg
     3601 cgccgaaacc cggcagcgag gctgagatgg gcagctcttc cggcacgcgc ttcgcatccg
     3661 cctcggcgag ttcgtattcg acgtagatgg acagtgccgc gatcttttcc gacagatgcg
     3721 cggccagtgc gcgccgctcg tgcacgctca ggcgcggtcc gggctgggac atgagttcga
     3781 tcgccgcggc gatcgcgtcg atttccgcga agccgaatgc ttcggcggca ctcgaaatgt
     3841 ggtgcgccat gcgttcgatg tgagccacgg ctgccggctc tatcagcatc ggttcgtccg
     3901 gcagacaggc gcgcatctgc gcgacctccg cctgggcccg tgtgagaaac cgcagcgaca
     3961 acgcgcgctg cgacatctgt gtcgtgctcg tatcacctga ctgggacaag ctaccctcct
     4021 tgggcacacg tgaagtcttg catggcgtag cgcggaatat cggccgggtg ggcgcgcgct
     4081 cgcggtttat cctcccaatc tgtcggctgc gtctcactgt ctcgtgtcgc cgccggcgag
     4141 tcagcgactg actaccggac atgccctatc tagtgaaacc gcttacatcg atgactccgg
     4201 cgacaggatt tcatgacacc ggtggccacg ttcctgtcct aacaaccttg cgagtggaca
     4261 gtcggcgcga cgtgcgcttc cggcgaattt gaaagaaata ccaacgcctg acacgccgcc
     4321 aattccgtgg tgattccgcc acgatccttg gaagtgggga aaacgccgct atgatccccg
     4381 ccacacttct accggctggg aattggtgac cacgaaaccc gacgaaacag gcatccaaat
     4441 cgacgtcgcc gccgtccaga aaatactgtc cgtaccgacc attctcgatg tcgtgtgccg
     4501 caccacgggc atgggcttcg cagccattgc ccgcgtcacc gatgaccgct ggattgcctg
     4561 tagcgtccgc gacgaaatcg ggttcggcct cgcggctggc ggcgagctca acatagaaac
     4621 cacgatctgc aacgagatcc gccagcaccg cgagcctgta gtcatcgagc acgtcgccga
     4681 ggatcgcaag tttttcaacc accccactcc ggcgctgtac ggcttccaga gctacatctc
     4741 gatgcccatc atcctgccgg gcggcgaatt cttcggcacg ctttgcgcca tcgatccgaa
     4801 gcccgcgcgc gtgaacacgc ccgcgatcgt gaacacgttc cggctcttcg ccgaactcat
     4861 cgcatttcac ctggatgcgg atcgacgcct ggcggtcagt gaggcggcgc tgctcaacga
     4921 gcgcgagacc gcacgactgc gcgagcaatt catcgccgtg ctgggacacg acttgcgcaa
     4981 tccgctgggc agcatctcga gtgccgcaca cgttctcgcc aggacaccgc tcagcgagcc
     5041 cgcggccaaa ctggtggaga tgatgcagcg aagcgttcga cgcatggcgg gtctcatcga
     5101 cgacgtgctc gacttcgcgc gcggccgcct cggcggcggc ctcgcgctgt cgctgaccgg
     5161 cgatgggagc ctggcgccgg tgctcgagca ggtgatcgcc gaactgcgcg tgagttttcc
     5221 cgaccgcgtc atcgaaaccg acttcactcc gcaggattgc gtgaagtgcg atcggccgcg
     5281 tctgtcgcaa ttgctctcga acctggtggc gaacgctctc acctatagcg cgccggacac
     5341 gccggtgcgg gcgagcagcc gcatcgtcga cgggcagttc gagctcaccg tcaccaacca
     5401 gggcgaaccg atctcgccgc agatcatgga gcggctcttt tcgccgtacg aacgcggcgc
     5461 cgtgcgcccc agccagcagg gactgggcct gggtctctac atcgcttcgg aaattgcgcg
     5521 cgcgcatggt gggacgctca ccgtgacctc atcgcccgaa gagacttgct tcacgctcac
     5581 gattcccacg taagatcgcg cccatgtaca ccgaaatgtt cgcgcagatg aagaagcagc
     5641 tgggccagct cgacaagtgg ctcggcaccg cgagtgaata cgccaaggcg aagccgttcg
     5701 acccgaacgt attcgtgggc cagcgactgg cgcccgatca atttccgctg gcgcggcaaa
     5761 tccagatctg ctgcgacacg gcgaaactcg ccgcgtcgtt cctgaccggc aaggccgccg
     5821 aatcgcagcc cgacaccgag cagacgctcg aagagctgca gacgcgagtg cgctcgatca
     5881 ttacttacct cgatggaatc tcgccgcaag atctggaagg cgcggccacg cgcgtcgtga
     5941 gccagccgcg ctggaaaggc gaatggatga cgggcgccga ttacttcgtt cagcacgcca
     6001 tcccgaactt ctatttccac ctcaccaccg cttacgccat cctgcgtcac aacggcgtca
     6061 gcgtcggcaa gcgcgatttc ctgggcgcgc agaccaaacg tccgccggct aattgatatc
     6121 gcaccaacgc cgtttggaca ttggactcgc gggtggggtc aggcaccttt cgcggcatga
     6181 aaccagcgct cgcgattcgc gcatattgtc tggtcggcag cgtcatgctg gctgcctgcg
     6241 gtggtggcgg cagcgttagc ggctcgagca gcagctcgtc gtcctcttcc tcttcgtcat
     6301 cttccggcgg ttcgacaccg ccgccggcga tcacgttcaa caacgcgtcc gtgcacgatc
     6361 cgtcgatcat caaggtcggc tcggaatacc tcgtgttcgg ctcgcacctg gcggcggcga
     6421 agtccaccga tctcatgaac tggacgctga tcgcggatgg cgtcaccaac acgaacccgc
     6481 tgttcaccaa tgtcgtgacc gcgctccagc agaccttcac gtggtcgcag gtcactgact
     6541 tgtgggcgcc cgatgtcgtg cggctggccg acaacaagtt ctacttctat tacaactcct
     6601 gccgcggcga cgcgccgctg tccgcgctgg gcgttgcggt ggcggactcg gtcgtgggcc
     6661 catacgtcaa caagcagcag ttcctgcgct ccggcatgac gggactcagt gacgacggca
     6721 tcacctccta cgatccgctg gttcatccga acgtcgtcga tccgcacacg ttcttcgatg
     6781 cgaccgggaa tctgtggatg atctacggct cgtattcagg cggcatctac atcctcgcga
     6841 tggatgaaat caccggcctt ccggaagccg gccagggtta cggcacgcac ctgatgggcg
     6901 gcaatcacgc gcgcatcgaa ggcgcgtatg tgctgtacaa cgcgcagacc ggcttctact
     6961 acctgttcac atcgtttggt gggctggccg cgaacggcat gtacaacatc cgcgtcgcgc
     7021 ggtcgcaggc gcccgacggg ccctacttcg atgcagcggg cacggacatg gcaaccgtga
     7081 ccggcaaccc tgccgcggcc ttcgatgaca cgcgcatcgc accacatggg cagaagctca
     7141 tgggcaacca tcagttcgcg ctggccaccg gcgaatccgg cacggcgctc ggatatgttt
     7201 cgccggggca caactccgcg tacttcgacg cggggctcgc caaatacttt ctcatcttcc
     7261 acacgcgctt tccgaaccgc ggcgagcaac atgaaatccg tgtgcacgaa ctgctgttca
     7321 attcgcaggg ctggccggtg gcggcgccgt tccggtatgc gccgctgtcg ttgagcccga
     7381 ccgcggtggt ggccgaggtc accaatgccg acgtggcagg cgcgtacaag ctcatcgatc
     7441 acggcaaaga catcagcgcc accatcaagc tgtcgcaggc aatccgcctc aacgccgatg
     7501 gcacggtgag cggcggcgtc accggcacgt ggatccatcg cggcggtaac ctgatcgacc
     7561 tcactaccgg cggctcgctg tactacggcg tgttatcgcg ccagtggaat accaacgcca
     7621 accgcttcgt cgtgacgttc agcgcgcaat ccgtcgctgg cgtgtctctg tggggcgcgc
     7681 gcaccggcga ctgacggaaa tggggtcagg ccccatttac tgggcggtgg tgttcgtcgg
     7741 ggcgctcggg cgcacaccgt cgggatcgac atcggacaac acgacgttgt cccagcgctg
     7801 cgtgccgttg gtcacgaggt cggtcacgta gttgccgacg atgagcttgt taggcaggaa
     7861 gttggtgttc tcgcgcaccg cgacgctggt gcggtcggtc ttcagagcgc cattgaccca
     7921 catgcgcacg atgccgttcg actggccggg cgtgttgggc ttgatgaaca gcttgatgtg
     7981 ctcccagaca cctcggcgca tgatcgcatc cggcggcaca ttctggtcga tgccgggaat
     8041 ggtcttgttg aagctgcggt ccgcgttcca gaccacgtgc tccaggaagt attcgccggt
     8101 cggcgtccag atgttgacga tgatctggta acggcggcgc gcattctcgt cgtcgaagtt
     8161 gacgagcatg atcttgtgca ccgggttgct gccgaacacg aatccgttgt cgaacttcga
     8221 atcgaagctc agccacaatc cgcgatcgac gctgacggcc tcaccgccca ccgtcgggtg
     8281 attgccgaaa tagaaatcct tgtactgatc ggcgcccgtt ccggcgatga ccgtgccgcg
     8341 caccgaatgc gttccctgcg acgcatcttc cgtggtcacg acgagacggc ccgtggtgcc
     8401 accgttccat tcgctgaagg tgccggtttc gaagctgctg cggaaaggca ggcgcgcggg
     8461 agcggcctcc gcctgcgaaa cgatcgactg tgaaacgagt gcgattgcca gaccggcgaa
     8521 gaagctgcga cgcatggaaa gcctcaaata ctaagaagta agtcgcgtca gcatggatag
     8581 cgccgccggc atttgcaaag tgtctacaga cgcggacacc ccgtaagaac agcagttttc
     8641 cgcttcggct cgccatcgag gctcgttgta agaactacgt cacaacgagc cccgactttg
     8701 agggtatggc ctacaggatt tccacctggt cgaggttggc caggtcctgg ccgttcgatt
     8761 cgaaccggat cgtgttcacc gtgccggtgt tcagcgtgaa cgtcacgttc tgcgtcgccc
     8821 aggtagtcca ggcgcccgtg gggttgaacg tgatgttctg cgcgacaccg ttcaccagca
     8881 cccgccccgt ccggctggcg gtcgtgccca acgcgtaacg gatgcggatc gcatgactgc
     8941 ctccaccgcg cccgtcggca ttgttcacct gcgcgaaccc gcccgtggcg gagaagttcg
     9001 cgtagccact gccgatgaat ccgctgttgg tgctctcgct caccgtgccg ccgccgagtg
     9061 atgccgtctc gaactggtac gtcggcgtac ccggttgcgg atccggcggc gtgccgccga
     9121 ccagcgtgat gttaggcgtc ggcggcgtgg gcatgttggc gccaatgaag aagcccggat
     9181 gcggcggctg gttgtacgcc gtgttctgcc atgcgatcgc cacgcggtac tgcgggtcat
     9241 gcagcagcgt gtagatccgg ttgctcgcca cgttcgtcgt ggtgtaaatg cgcaacgcac
     9301 cgttgttggt ggttcgccag acgacttctt cacgccagtc gcccaacagg tcgccgctca
     9361 acgccggatt cgacttggtg ccgttgctgg tcgtggtccc atccgccgtg agcaggcgcg
     9421 tgtcgccgcc cgtgccgtac ttgtcgacgt gattgccatc caccagctcg cgcaccgtgt
     9481 ctgcgtccca ccaggcaaga tggttcgccg aacccggatt ccggccgatg ttgcctcccg
     9541 acgcattgcg caggaacgtc atgttggggc ccgcgcccca atactccgcg ccttcgttgc
     9601 ccgaccagat gtcggcggcc acgccgcgcc ccgggccttc cactccattg ttgttaggcg
     9661 tcgcgaagat gcgcgcgccg gtgcgcgcat cgcgcaggtc ggcgccaggc tggttgctcg
     9721 actcgtggat gtcccagatc tcgagtccgg cccgtgacgg aatgaagtcg cccacgtgca
     9781 gcgcgtctcc atgcccgagg ccactcgtgt acatccgcgc gccgttatcg tcgatggtcg
     9841 cggcgccgaa gatgacttca tggcgtccat cctggtcgac gtcggcgatg gacaggttgt
     9901 ggttgccctg ccccgcgtag gcgctgttgc ccgagccgtt gctgtcgaac acccagcgtt
     9961 gcgtgagctg gccattgcga aaatcccagg ccgcgatcac ggcgcgcgtg taatagcccc
    10021 gcgagaagat ggcgctgggc cgctggccgt ccaggtaggc cacgcccgcc aggaagcggt
    10081 ccacgcgatt gccataggtg tcgccccagt cgcccacgtt gccgcgcgcg ggaacgtagt
    10141 tggtggtcgc gagcaccgct ccggtctgac cgttgaacac ggtgaggaac tccggccccg
    10201 cgaggatgta gccactcgaa ttgcgatggt cggccgtcga actaccgatg acctggttcg
    10261 tgccactgcg cgtgccatcg gcggtcttgc acatgacctc ggcacgtccg tcaccatcga
    10321 agtcgtacac catg
//
LOCUS       DATDEU010000113         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1625805, whole genome shotgun sequence.
ACCESSION   DATDEU010000113 DATDEU010000000
VERSION     DATDEU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625805"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1743
                     /locus_tag="VIV63_02760"
     CDS             121..1743
                     /locus_tag="VIV63_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6123547.1"
                     /translation="MPLVAAVPPAPTDLAWRVIGLVNLYRLLIPPALFLLHLYFGATT
                     PIGNAYPELFLWACVVYFGAGVAIVFGGRRMLPNLRATTFVHAMVDAVAISLLLFSSG
                     GVASGIGILFVVPVGAMALLADSRDAFVLAALATLALFAQQVASHAVGHAVVSDYPTA
                     GILGGIVFLVALLAWPIARRLRDTEATVRRQQVDLANLAQLSLYIVQTLRESIVVVDQ
                     ENRIRLINESAAVLLGDRSAYPGALLGEASPQLLYLLETWRQRAAAPVVPSQTFVAAD
                     GGHVIQPHFASLGGTEPAPVIVFLEDTELLAAKIQQSKLAGLGRLSASIAHEIRNPVG
                     AMSHAAQLLAESPSLSDEDKRLTEIVRTNGDRVRQIIENVMSMARRESSRPERIILAT
                     WLAGFRDEFSATMQIKPERLAVSSLLGDVEVQVDPTQLRQIVWNLCENAAKYGVGDAG
                     ADTIELRIGRMASTARPFLEVADRGPGIPAKNRERIFEPFFTGNERGTGLGLFLAREL
                     AETNGATLLYEPRTSGGSLFRIVFRDPERWVA"
     gene            1747..>2160
                     /locus_tag="VIV63_02765"
     CDS             1747..>2160
                     /locus_tag="VIV63_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6123548.1"
                     /translation="MAQPLVLVVDDEPDLVELVTLTLARMQLATQSAGDVASAKKLLK
                     TQHFDLCLTDMRLPDGDGLDLLEWMSTNCPGVPCAVITAHGNVESAVRALKLGAFDFV
                     SKPLDLGVLRRIVSTALKLSQRTDGSVTTSTGSHII"
BASE COUNT          337 a          721 c          692 g          410 t
ORIGIN      
        1 acacttcttt tgactggtac tcgtggaggt tcatcaggcc ctggcgttgg ttgcgaaaaa
       61 agaggcggta ttctagcggc aactcgtccc gcctgcggct agctgttaca gtcgcgcccg
      121 atgccgctag tcgccgccgt accgcccgca ccgactgacc tcgcctggcg agtcattgga
      181 ctggtcaacc tctacaggct gctgattccg ccagcgctgt tcctgttgca tctctacttc
      241 ggcgcgacca ctccgatcgg taatgcatac cctgagctgt tcctctgggc ctgcgtggtc
      301 tatttcggcg ccggcgtggc cattgtcttc ggtggccgcc ggatgttgcc caatttgcgg
      361 gcgaccacct tcgtccacgc catggtcgac gccgtggcga tcagcctgct gctgttttcg
      421 agcggtggcg ttgccagcgg catcggcatc ctgttcgtcg tcccggtcgg cgccatggcg
      481 ctgctcgcgg acagccgcga tgccttcgtg ctggcggccc tggcgacact cgcgttgttc
      541 gcccagcagg tcgccagcca tgcggtcggt catgcggtgg tctcggacta cccgacggcc
      601 ggcatcctgg gcggcatcgt cttcctggtg gcgctgctcg cctggccgat cgcgcgccgc
      661 ttgcgcgaca ccgaggccac ggtgcgccgc cagcaggtcg atctcgccaa cctcgcccag
      721 ctttcgctat acatcgtgca gacacttcgc gagagcatcg tggtcgtcga ccaggagaat
      781 cgcatccggc tcatcaacga gtccgcggcg gtgttgctgg gcgatcgcag cgcctatcct
      841 ggcgcgttgc tcggcgaagc ttcgccgcag ttgctctacc tactcgaaac ctggcgccag
      901 cgtgccgcgg caccggtggt gccttcgcag accttcgtgg cggccgacgg cggtcacgtg
      961 atccagccgc attttgcgtc gctcggcggc accgagcccg cgccggtcat cgtgttcctc
     1021 gaagacacgg agctgctcgc tgcgaaaatc cagcagtcga agctcgcagg tctgggcagg
     1081 ctgtcggcga gcatcgcgca cgaaattcgc aatcccgtgg gcgccatgag ccatgcggcg
     1141 cagttactcg ccgagtcgcc ctcgctctcc gacgaagaca agcgattgac cgagatcgtg
     1201 cgcaccaatg gcgatcgcgt gcgccagatc atcgagaacg tcatgtcgat ggcgcgtcgt
     1261 gagagctcgc ggccggagcg catcatcctc gccacgtggc tcgcgggttt ccgcgacgag
     1321 ttcagcgcga cgatgcagat caaaccggaa cggctggcgg tttcctcgct gctcggcgat
     1381 gtggaagtcc aggtggaccc gacgcagctg cgccagatcg tctggaacct gtgcgagaac
     1441 gccgcgaagt acggcgtcgg cgacgccggt gccgacacca tcgagctgcg aatcgggcgc
     1501 atggcctcga ccgcgcggcc attcctcgaa gtggccgacc gcggccccgg cataccggcc
     1561 aagaatcgcg agcggatttt cgaaccgttc ttcacgggca acgaacgcgg cacgggtctg
     1621 ggcctgttcc tggcccgcga gctggcggaa accaatggcg ccactcttct ttatgagccg
     1681 cggaccagcg gcggcagcct gttccggatc gtctttcgtg atcccgaacg ctgggtagcg
     1741 taaaccgtgg ctcagccgct cgtcctcgtc gtggatgatg agcctgacct cgtcgaactg
     1801 gtcacgctca cactggcgcg catgcagctc gcgacgcagt ccgcgggcga cgtggccagc
     1861 gccaaaaaac tcctcaagac gcaacacttc gatctttgcc tgacggacat gcgattgccg
     1921 gacggcgacg ggctggacct gctcgagtgg atgagcacga actgcccggg cgtcccctgc
     1981 gctgtcatca cggcgcacgg gaacgtggag tcggcggtgc gggcgctcaa gctgggcgcg
     2041 ttcgatttcg tgtcgaagcc tctcgatctc ggcgtgttgc ggcgcatcgt gtccacggcg
     2101 ctcaaactgt cgcagcggac cgatggcagc gtcaccacga gcacgggcag tcacatcatc
//
LOCUS       DATDEU010000114        12595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1771987, whole genome shotgun sequence.
ACCESSION   DATDEU010000114 DATDEU010000000
VERSION     DATDEU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771987"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VIV63_02770"
     CDS             <1..660
                     /locus_tag="VIV63_02770"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123549.1"
                     /translation="REMVLRLAERLEVPLRERNELLVSAGYAPLYSSRPLDDPAMNSA
                     RAAVDLILNGHDPYPAILVDRYWNLLAANKAAGAFMVGVDPSILGPPVNVLRATLHPK
                     GMAPRIENLAEWRTHILTSLARVLELSADPTLAELLTELRGYPGGEAEPDPQFGGVVV
                     PLKLRTEAGTLSMFTTTTVFGTPVEVTLSELMLEAFYPADEFTATVLRQIAVNPGPAT
                     P"
     gene            complement(657..3632)
                     /locus_tag="VIV63_02775"
     CDS             complement(657..3632)
                     /locus_tag="VIV63_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123550.1"
                     /translation="MRVSSFGLVAGTGVVALSIVGPASGQDAATPAEAMDEVIVTGSR
                     IQNRDYEATSPVVTIAADAFELSGEVQVESVLNTLPQLVPSISTTSNNPSNGGQANID
                     LRGLSGNALAPRTLVLVDGSRLTPSNPTGVVDLNTVPTALIDGVEILTGGSSSTYGSD
                     AIAGVVNVRLKRDFEGIQISTQHNVTEQSDGSSTLIEALMGGNFGDSDRGNAVLALSF
                     DKRDAVLAGDREFGVVSRGPLLTPVGSGTVPDGRVDWGANGPTAAAVNQVFGAYGAPA
                     GSVMPTSSIGMNPNGSLFSFGSGTAANPVVNYLGDTENPGYNPLAYSYNFGPINYLQL
                     PINRKQASAFLRYEAVPEHAEIYSRLMYTTFHSDQQLASTPVTCSGAALGCSIPVTNT
                     AIPADLRALLDSRTNNTAPLAFTRRYTEVGPRTQENRFDVVQTLLGVRGDFNVGERDF
                     SYDVYGSWGQVSNPALQGGNVSRSRLQAALNNPAVYASRGCAQFNPFGEGSITPECAA
                     AISIKATNIFDLTQTNFVAAVTGSVFTLPAGDVKFAVGGEYRDNDATFRPDEYLASGD
                     VVGFNAAQPVDGKIEVTEGFAELSIPLLHDLPAVQTLGLDLGYRYSNYNIAGAADTYK
                     AALQWSPVESLSVRASYNRAIRAPSIAELFLPRQENFPQYSDPCNFNSSFRTGPNAAQ
                     VRALCEAQGIPASLLPTYTQLNSQARAIIGGNQELTPETADTYTFGVTWQSPSDSGWF
                     PGMNVSVDYYSYDIRNVIQALSASSVVGRCFNQLDSNPTFDASNEFCQLFSRNPSNFV
                     VTDVSTLSENLSGLKQTGVDLGLDWSLPLSRFGASEAAGELGFRLLLTKLLTMEQQET
                     SVDPFIARQDTISQTVASAFPNSKGVLTTMWSKGGWQLRYNLRYINSMDVVNNNAILS
                     RPAVGVAPHVPSYTYHDLVARWAMNETFGFTVGATNLADKQPPIYTTDSQAGIQSNTD
                     PSTYDVLGRRYFLNVTMNF"
     gene            3796..4926
                     /locus_tag="VIV63_02780"
     CDS             3796..4926
                     /locus_tag="VIV63_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6123551.1"
                     /translation="MSSSGTTIETRPGLSLRATLTIATLFWVYSCVAVILRLELTRRG
                     LSGKGPGIGVLATTCLLLYPVLCALTYCSWRLGFDGARWVRVVLVNIALACIFGVASR
                     PAYILSNAIHNDRTIAETQIRIDGVNFDKAVKLWGSQFVEDAAIYLMLQAMLFGASAF
                     VRLKDESAARDRLAHEYDRARLQALRMQTNPHFLFNTLSAIAGLINARPAAAQSMVTQ
                     LGELFRATLEDRDTELVSLQRELELGQQYLEIQRTRFDERFDYAFNVQPDAGNVAIPP
                     LLLQPLLENAAEHGLTTQDGSVLVEVDAECEEGTVRIVVRNRASLASAGRRGSHRGLG
                     LELVRERLLAAFGPAAGLTTELTDGGTFLARLTFPRHEMALA"
     gene            4923..5726
                     /locus_tag="VIV63_02785"
     CDS             4923..5726
                     /locus_tag="VIV63_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY6123552.1"
                     /translation="MNEQAPIRTLIVDDESPARQWLRTLCARIPDVRIVGECATASEA
                     SRALRTSSIDLLLLDIQLGAHTGFQVLDGVAYSAVPNLVFVTAHDKYAVRAFDRRAVD
                     YLLKPVREDRFRESLERVRRQLRGGMASEIQSAVRETIMPLEQSLLSLRTHGYTDRLV
                     GERGGAYRIIECRNMVSLESEGNYVRVVEQGCAEPAIMRGTLQSLVAILDPEQFVRVS
                     RFAILNLTFVDRIERDADSHLVFIMRGDSRSFSVGRAYAADVQRVLRLG"
     gene            5806..6495
                     /locus_tag="VIV63_02790"
     CDS             5806..6495
                     /locus_tag="VIV63_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123553.1"
                     /translation="MQTLIRAAVVIAVAGTLFACKPKPEAKPVAEAEATDDSEAGPGE
                     NLLEACVITMTEPELVRWTTYWDAAAGVNNTESESIARSEFWANDEEKKSLSRSSSAA
                     PLTIQCSSNDEPSIAVSFSSYSSTMNDIPLGSGEYAIVSSSGGDPQPGQFQAGPVLYD
                     QRTIAVTGGTLTIDRFDMEGVRGAFRVDGKADGEDGEEGQEYRFEGTFEIPCRGGSME
                     SACESNKAQTN"
     gene            complement(6509..7399)
                     /locus_tag="VIV63_02795"
     CDS             complement(6509..7399)
                     /locus_tag="VIV63_02795"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6123554.1"
                     /translation="MLPTLVTADMRYWRIAPDPRLREHVLCYWMVEDTPQRASDARFQ
                     HGEDLLLPDGYSEIVFNRTSVSFERWQLGARADSTEMRGSYVIGGRSKSVGTHTSAPL
                     QLAGVKLDPRFLRDIIGVPLHEFRDSTLPLRNLRHAGLNDLEEQIAESSGPTRMAALL
                     DGFLLGALKRAWRPSTATDALLHRIHRDRGSTPIMHWARDASVDARSLERAFCAATGM
                     TPKQYARVIRFKRAYHSLLSERRPLTSALDGFYDQSHFNREFRHFMGVSPGVRLGDSM
                     NQATNVTDHLLQADLGATAN"
     gene            7471..7920
                     /locus_tag="VIV63_02800"
     CDS             7471..7920
                     /locus_tag="VIV63_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6123555.1"
                     /translation="MKYQLTVNGTRHAVDVDADMPLLWVIRDFVGLTGTKFGCGLGLC
                     GACTVHLDGIAIRSCSVPVSAAAGKALTTIEGLSPDGQHPLQRAWEEGDVPQCGYCQA
                     GQIMQAASLLREKPKPTDAEIDDAMAGNLCRCGTYQRIHRAIRKASE"
     gene            7917..10115
                     /locus_tag="VIV63_02805"
     CDS             7917..10115
                     /locus_tag="VIV63_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6123556.1"
                     /translation="MKRREFLRDSLLVSGGLTLGFSVVANEVRTSAAAAGITPIGDFL
                     RITPAGDVLFQVVKHEGGQGVATALAQILCEELAADWSRVHVEFPMADMARWQNDRNG
                     GHDTGGSCTITYQYDLLRKAGATAREMLVAAAAKQWGVPAATCRVENHAVVHAASARR
                     SGFGELASLASQMPVPADVALKSDKQQTLIGQGKAAKIIPDITSGRLKYGIDTQVPGM
                     MYALIARCPVFKGRLRRFDASKALAVKGVRKVFAMTPVAGHHEPPYMPHDIRDGVAVV
                     ADSFWAARKGRDALQIEWDEGEKARFSSEDFARLAADRARERKDPTGFIGDDNAVNDL
                     TRVRKTLRASYIYPHQVHSCMEPLNCTAFVRKDDCEVWCGSQAPNLIVGELAKLLKLP
                     PEQIRVHLLPSGGGFGRRYYPDFAVEAAFISREAGNVPVKMMWTREDDQTVNLAHHYQ
                     HMEYQTAIDGANKLFAWYEKEIRTYTWGSHYAGPELPQMAYDIPNIRYDFEELNDEEL
                     VQSCAWRGVVSHGRSLSECFVDEIAAELKRDPLEFRLSLLTRGRDAVVSEGGRISSDR
                     MTRVLALAAEKAGWGGELAAGRGRGICLSPYGNTCVAAIAEVTVKDGALRIDRVTVAV
                     DCGRLINPSGAANQIEGGIVWSLTGLLYGGLPIRNGRAVNRTFAENKLLRMNECPKID
                     VHFVGGDAERPWGIGEVSTPVGAPAVLNAIFSATGKRLRTLPIEGQSLTS"
     gene            complement(10112..11416)
                     /locus_tag="VIV63_02810"
     CDS             complement(10112..11416)
                     /locus_tag="VIV63_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY6123557.1"
                     /translation="MPTSPPQTADDRLLRGIGRWALTAFAINLTVGSGILGLPAKIQA
                     LVGNYSVPIIIACGLLMALIALCFAEVGSRFDRTGGPQLYASQALGPTAGFAVGWLLW
                     ISRIGSCAAVSNLAVDYGKVLWPALSHPGVRALAITLLVLGYTWINIRGIRQTAAVNT
                     TFTVLKIVPLVAFVAIGLLFVEPQALRLEELPAITDLSTALLLAAYAFVGFDGTTVLA
                     GEVRDPRRSVPFAILFSVACVIVLYTLIQVVCVGTLPNLAASERPLADVALLLVGPAG
                     AVVIAIVAVISCAGVFGASMTPGTRLLFSMGDDRQLPHLLAYVHKRYRTPVIGILVTA
                     VSILALAISGSFIYLVKVTLIARLSIYAITCVTLPIFRRMPNTPAAQFTLPGGSLVAV
                     ASALLCVGFLANSSMRELLDVGVATAGGLAIYFVTRRVYTPD"
     gene            11516..>12595
                     /locus_tag="VIV63_02815"
     CDS             11516..>12595
                     /locus_tag="VIV63_02815"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6123558.1"
                     /translation="MTHSRRQFLEISLLSGGGLLVSFALPEFARAATNERPGPSSVPL
                     GAFVRINADNSIEIGARGCEIGQGVITSLPMLIAEELDVRWDQVTVKQLPYGIMAGKE
                     PGSLAPRYGGQGAGGSTNITDARTELREAGVQIRQLLVAAAAQLWTADAATLKTRDAI
                     VSHPDGRTATYGSLAARAAQMPLPAGPFELRKPADFRIIGKPTKTADCSDIVAGAARY
                     GIDAQMPGMLYAVIARCPYFEGTLKSLDDTAAKKVRGVRAVVPIAPPPAGPLSRNLAA
                     GVAVIADNTWAAMQGRKALKIEWTPGPWAADSTQALEARCRAAVAGDANIQKGRSDGD
                     MLAAWAGAARKIEADYKVPFLAHSTM"
BASE COUNT         2295 a         3940 c         4239 g         2121 t
ORIGIN      
        1 cgcgagatgg tattgcgact ggcggagcgg ctcgaggttc cgctgcgcga acgcaatgag
       61 ttgttagtgt ccgcgggata cgctccgctc tattcgtcgc ggccgctcga cgatcccgcg
      121 atgaactcgg cgcgcgcggc cgtcgacctg atcttgaatg gccacgatcc ttatccggcg
      181 atactggtag accggtactg gaatctgctg gcggcgaaca aggcagccgg cgcgttcatg
      241 gtgggcgtgg acccctcgat actcggtccg cctgtcaatg tgctccgcgc cacgcttcat
      301 ccgaaaggca tggcgccgcg tatcgaaaac ctggccgaat ggcgcacgca cattctgacg
      361 agcctggcgc gcgtgctcga actctcggcc gatccgacgc tcgcggagtt gctgacggag
      421 ctgcgcggct atccgggcgg cgaagcggag cccgatccgc aattcggcgg agtggtggtg
      481 ccgctcaagc tgcgaacgga ggcgggcacg ctatcgatgt tcacgacgac gacggtgttc
      541 ggaacgcctg tggaggtgac gctctccgaa ctcatgctcg aagcgttcta tccggcagac
      601 gagttcacgg cgaccgtcct gcgacaaatc gccgtgaacc cagggcctgc caccccttag
      661 aaattcatcg tcacattcag aaagtaccgc cgccccaaca cgtcatacgt ggaaggatcg
      721 gtgttcgact gaatgcccgc ctggctgtcg gtagtgtaga taggcggctg cttgtcggcg
      781 agattcgtcg cgcccaccgt gaacccgaac gtctcgttca tggcccagcg cgcgaccagg
      841 tcgtgatacg tgtacgaggg cacgtgcggc gccacgccca ccgcgggcct ggaaaggatg
      901 gcgttgttgt tcaccacatc catggaattg atgtagcgca ggttgtagcg aagctgccag
      961 ccacccttgg accacatcgt ggtcagcacg cccttggagt tggggaacgc actggcgacg
     1021 gtctggctga tggtgtcctg acgcgcaatg aacgggtcca ccgaggtttc ctgctgttcc
     1081 atcgtcagca acttcgtgag caacaggcgg aagcccaact ctccggcggc ttcgctggcg
     1141 ccgaagcgcg agagcggcaa gctccagtcg agtcccagat cgacgccggt ctgcttgagg
     1201 ccggagaggt tctccgacaa cgtgctgacg tcggtgacga cgaagttcga gggattgcgg
     1261 ctgaagagct ggcagaactc gttcgacgcg tcgaaggtcg ggttggagtc caactggttg
     1321 aagcatcggc cgacgacgga gctggcactg agcgcctgga tgacattgcg gatgtcatag
     1381 ctgtagtagt cgaccgagac gttcatgccg ggaaaccagc cggaatcgga cggcgactgc
     1441 cacgtgacgc cgaacgtgta cgtgtccgcg gtctcgggcg tcagttcctg gttgccaccg
     1501 atgatcgcgc gcgcctggct gttgagctgg gtgtaggtcg gcaacaggct ggccgggatt
     1561 ccctgcgcct cgcacaacgc gcgaacctgc gcggcgttcg ggccggtgcg gaaactcgag
     1621 ttgaagttgc aggggtcgga gtactgcggg aagttctcct gccgtggcag gaacagctcc
     1681 gcgatgctgg gcgcgcggat ggcgcggttg tacgacgccc tcacgctcag cgattcgacc
     1741 gggctccact gcagcgccgc cttgtaggta tccgcggcgc ccgcgatgtt gtagttggag
     1801 tagcggtagc ccaggtcgag accgagcgtc tgaaccgcgg gtagatcgtg cagcagcgga
     1861 atggaaagct cggcgaaccc ttcggtcacc tcgatctttc catccacggg ctgcgcggca
     1921 ttgaagccga cgacatcgcc gctcgccagg tactcatcgg gccggaacgt ggcgtcgttg
     1981 tcgcggtact cgccaccgac ggcgaacttc acgtcaccgg caggcagggt gaacaccgat
     2041 ccggtgaccg cagccacgaa gttggtctgc gtgaggtcga agatgttggt tgccttgatg
     2101 ctgatcgccg cggcgcactc gggcgtgatg ctgccttcac caaacgggtt gaactgcgcg
     2161 caaccccgcg aggcgtagac agccgggttg ttcagcgccg cctgcaaccg cgagcgcgac
     2221 acgttgccgc cttgcaaagc cggattgctc acctggcccc agcttccata cacgtcgtag
     2281 ctgaagtccc gctcgccgac gttgaagtcc ccgcgaaccc ctaacagggt ctgcacgaca
     2341 tcgaagcgat tctcctgcgt gcgtgggccc acctccgtgt agcggcgcgt gaacgcgagt
     2401 ggtgcggtgt tgttggtgcg cgagtccagc agcgcacgca ggtcggcggg aatggccgtg
     2461 ttggtcacgg gtatcgagca accgagtgcg gcgccagagc aggtcacggg tgtcgacgcg
     2521 agctgctggt cggaatggaa cgtggtgtac atcagccgtg aatagatttc ggcgtgctcc
     2581 ggaacggctt cgtagcgcag gaacgccgaa gcctgcttgc ggttgatagg caactgcagg
     2641 tagttgatgg gcccgaagtt gtacgagtac gcgagcgggt tgtaacccgg gttttcggtg
     2701 tcgccgagat agttcacgac gggattggcg gccgtgccgc tgccgaacga aaacagcgag
     2761 ccgttgggat tcatgccgat ggagctggtg ggcatcacac tgccggcggg tgcgccgtag
     2821 gcgccgaaca cctggttcac ggccgcggcc gtcgggccgt tcgcgcccca gtcgacgcgg
     2881 ccgtcgggga cggttccgga gcccacgggc gtgagcagcg ggccgcgcga cacgacgccg
     2941 aattcgcgat cgcctgcgag tacagcgtcg cgcttgtcga agctcaatgc cagcaccgcg
     3001 ttgccgcgat cggaatcgcc gaagttgccg cccatcaacg cctcgatcag cgtggagctg
     3061 ccgtcgctct gctcggtcac gttgtgctgc gtggaaatct ggatgccttc gaagtcgcgc
     3121 ttgaggcgca cgttgacgac accggcaatc gcgtccgatc cataggtgga cgacgagccg
     3181 ccggtgagaa tctccacgcc atcgatgagt gcggtcggca cggtgttgag atccacgacg
     3241 ccggtcgggt tcgacggcgt gaggcgcgag ccatcgacca gcaccagtgt tcgcggcgcg
     3301 agcgcattgc cgctcagacc gcgcaggtcg atgttggcct gtccgccatt cgacgggttg
     3361 ttagaggtgg tggagatcga cggcacgagc tgcggcagcg tattgagtac ggattcgacc
     3421 tggacttcgc cggagagctc gaacgcgtcc gcggcaatgg tgacgaccgg gctggtcgcc
     3481 tcgtagtcgc gattctgaat tctcgatccg gtgacgatga cctcgtccat cgcctccgcg
     3541 ggtgtcgcgg catcctggcc cgaagccggg ccaactatcg acaacgccac cacgcccgtg
     3601 cccgcgacca gaccaaaact tgaaactcgc atgtctcttt ctcccaattg ttatgagttt
     3661 cggaagggtc tcctgtctat tccgcaacga atgcctgcgc cagcgcgaag ccgttcgtcc
     3721 cagcgggcgg tcactcggca cttgtcatag tcacttcgtt cgaaaccgca tgattaccgc
     3781 tagaattcgg ccatcttgag tagcagcggc acaacaatcg aaacacgccc ggggctgtcg
     3841 ctgcgcgcca ccctaactat cgcgacgctg ttctgggtgt actcctgtgt cgcggtgatc
     3901 ctgcggctcg agctcacgcg tcgcggcttg agcggcaagg ggccgggtat cggcgtgctg
     3961 gcgacgacgt gtcttctgct ctatccagtt ctctgcgcgc tcacctattg ttcctggcgg
     4021 cttggattcg acggcgctcg ttgggtgagg gtggtgttgg tcaacatcgc gctcgcgtgc
     4081 atcttcggcg tggcgtcacg tccggcctac atcctgtcga acgccattca caatgaccgg
     4141 acgatcgcgg agacgcagat ccgcatcgac ggcgtgaact tcgacaaggc cgtgaagttg
     4201 tggggatcgc agttcgtcga agacgccgcc atctacctca tgctccaggc catgctgttc
     4261 ggcgccagtg cgttcgtgcg gctcaaagac gaatcggcgg cgcgcgatcg cctcgcgcac
     4321 gaatacgacc gcgcgcgact gcaggcgttg cgcatgcaga ccaatccgca tttcctgttc
     4381 aacacgctga gcgcgatcgc ggggctcatc aacgcgcggc cggcggccgc tcagtccatg
     4441 gtcacgcaac tgggtgaact gtttcgcgcc acgctcgagg atcgggatac cgagctcgtc
     4501 tcgctgcaac gcgaactcga gctcggccag caatacctgg agatccagcg cacgcgtttc
     4561 gatgagcgct tcgactacgc cttcaacgtg cagcccgatg cgggcaacgt ggcgatcccg
     4621 ccgttgctgt tgcagccgct actcgagaac gcggccgagc atggtctcac cacccaggac
     4681 ggttcggtgc tggtcgaagt ggatgcggaa tgcgaggagg ggacggttcg cattgtcgtg
     4741 cgcaaccgcg cgtcattggc cagcgccggc cggcgcggct cgcaccgcgg actgggactg
     4801 gagctggtgc gagaacgctt gctcgccgca ttcggtcccg cggcaggcct cactaccgaa
     4861 ctgaccgacg ggggcacctt cctcgcgcgc ctcacattcc cgcggcacga gatggcgctg
     4921 gcatgaacga gcaggcgccc atccgcacgc tcatcgtcga cgacgaatca ccggcccgcc
     4981 agtggctgcg aaccctgtgc gcgcgcattc cggatgtgcg catcgtcggt gaatgcgcca
     5041 cggcgtccga agcatcgcgc gcgttgcgca cgtcttccat cgacctgttg ttgctcgata
     5101 tccaactggg cgcgcatacc ggcttccagg tgctcgacgg cgtggcgtat tcggccgtgc
     5161 ccaacctggt gttcgtgacg gcgcatgaca agtacgcggt gcgcgcgttc gatcgccgcg
     5221 cggtggatta cctgctgaag cccgtgcgcg aagaccgctt tcgcgagtcg ctcgaacggg
     5281 tgcggcgcca gcttcgcggt ggcatggcca gcgagatcca gtccgccgtg cgcgagacca
     5341 tcatgccgct cgagcagtcg ctgctgtcgt tgcgaaccca cggatacacc gaccgtctcg
     5401 tcggcgaacg cggcggcgcg taccgcatca tcgaatgccg caacatggtc agcctggaat
     5461 ccgagggcaa ctacgtgcgc gtggtggagc agggctgcgc cgagcccgcg atcatgcgcg
     5521 gaacgctgca gagtctggtg gcgatcctcg atcccgagca gttcgtgcgc gtcagccgct
     5581 tcgccattct caacctgacc ttcgtggacc gcatagaacg cgatgccgac agccacctcg
     5641 ttttcataat gcgcggggat tcccgctcct tcagcgtggg ccgcgcctat gcggccgatg
     5701 tccagcgagt gcttcgcctg ggatgacgcc tcccggcgga ggctaggtgc ccgcaggggt
     5761 ctgcgggtat gatcgcccgc gacacaggtc cattacggaa tccccatgca gacgctcatt
     5821 cgcgcagccg ttgtcatcgc cgttgcaggt accctgtttg cctgcaagcc gaagccggag
     5881 gcgaaacctg tcgcggaggc tgaagccacc gatgattccg aggccgggcc gggcgaaaac
     5941 ctgctcgaag cctgcgtcat cacgatgacg gagccggaac tggtccgctg gactacctac
     6001 tgggacgcgg ccgcgggcgt gaacaacacc gagagcgaga gcatcgcccg gtccgagttc
     6061 tgggcgaatg acgaagaaaa gaagtctctg agccgcagca gctcggcggc gccgctcacc
     6121 atccagtgca gcagcaacga cgaaccgagc atcgcggtct cgttctcttc gtacagctcc
     6181 acgatgaacg atatcccgtt gggctcgggc gaatacgcca tcgtgagttc atcgggaggc
     6241 gatccgcagc ccggacagtt ccaggcggga cccgtgctct atgaccagcg caccatcgcc
     6301 gtgaccggcg gcacgttgac catcgaccgg ttcgacatgg aaggcgtgcg tggcgcgttc
     6361 cgcgtggatg gcaaagcaga cggggaagac ggcgaggaag gccaggagta ccgcttcgaa
     6421 ggcactttcg aaattccctg ccgcggtggt tcgatggaaa gcgcctgcga atccaacaag
     6481 gcccagacga actgagatcg tccgcgtttc agtttgccgt ggcgccgagg tcggcctgca
     6541 gcaggtgatc cgtgacgttc gttgcctggt tcatcgaatc gccgagccgc acgccggggg
     6601 agacacccat gaagtgccgg aattcgcgat tgaagtgcga ctggtcatag aacccatcga
     6661 gcgcgctggt gagaggacgc cgctccgaca gcaaggaatg ataggcccgc ttgaagcgga
     6721 tcacgcgcgc gtactgtttg ggtgtcatgc ccgtcgcggc gcaaaaagcg cgttcgagac
     6781 tgcgcgcatc cacgctggcg tcacgggccc aatgcatgat gggcgtggag ccgcggtcgc
     6841 gatgaatcct gtgcagcagc gcatccgtgg ccgtcgacgg gcgccacgcg cgcttcaggg
     6901 cgcctaacag gaaaccgtcg agtagcgcgg ccatgcgagt cgggccagaa ctctccgcaa
     6961 tctgctcctc caggtcgttg aggcccgcat ggcgcagatt gcgcagcggg agcgtcgagt
     7021 cgcgaaactc gtgcagcggt acgccgatga tgtcgcgcag gaatcgcggg tccagcttca
     7081 cgcccgcgag ctgcagcggc gccgacgtgt gcgtgcccac cgatttcgag cggccgccga
     7141 tgacgtagct gccgcgcatt tcggtgctgt ccgcgcgtgc gccaagttgc caccgctcga
     7201 agctcaccga ggtgcgattg aacacgattt ccgagtagcc atcgggcagc agcaggtctt
     7261 caccatgctg gaagcgcgcg tcggacgctc gctgtggcgt gtcctcgacc atccagtaac
     7321 agagcacgtg ctcgcgcagc cgcgggtccg gggcgatgcg ccagtagcgc atgtcagcgg
     7381 tgacgagagt cggtaacacg cgccgatcgt atcagcgccg gcgccgaatt tttacaagac
     7441 aggcgtggcc gggaacgaaa tcctacgccg gtgaaatacc agctcaccgt aaacgggaca
     7501 cggcacgccg tggatgtgga cgcggacatg cccttgctgt gggttatccg cgattttgtc
     7561 gggctcaccg gcacgaagtt cggttgcggg ctggggcttt gcggtgcgtg caccgtacat
     7621 ctcgacggga tagcgattcg ctcctgctcg gtgccggtct cggcggcggc gggaaaagcg
     7681 ctgaccacca tcgaagggct ctcgcccgat ggtcagcatc cgttgcaacg cgcctgggaa
     7741 gagggcgacg tcccgcaatg cggttattgc caggccgggc agatcatgca ggccgcgtcg
     7801 ctgctgcgcg agaagccgaa gccgaccgat gccgagatcg acgacgccat ggctggaaat
     7861 ctgtgtcgct gcggcacgta tcagcgcatt catcgcgcca tccgcaaggc gagcgaatga
     7921 aacggcgcga gttcctgcgc gactcgctgc tcgtcagtgg cgggctcacg ctgggtttca
     7981 gcgtggtcgc gaacgaagtg cgcacgagcg ccgcggcggc cggcatcacg ccgatcggag
     8041 attttctgcg tatcaccccc gcgggtgacg tgctgttcca ggtcgtcaag catgaaggcg
     8101 gccagggcgt ggccacggcg ctggcgcaga tcctctgcga ggaactcgcc gccgactggt
     8161 cgcgggtgca tgtggaattc ccgatggcgg acatggcccg ctggcagaac gatcgcaacg
     8221 gcggccacga cacgggcggg agctgcacga tcacctatca atacgatctt ctgcgcaagg
     8281 ccggcgccac cgcgcgcgaa atgctcgtgg ccgcggccgc gaagcagtgg ggcgtgcctg
     8341 cggccacttg ccgcgtcgag aatcatgccg tcgtacacgc ggcttcagcg cgacgatcgg
     8401 gattcggcga gcttgcgtcg ctggcgtcgc agatgcccgt gccggctgac gtcgcgctga
     8461 aaagcgacaa acaacaaacg ctcatcggcc agggcaaagc cgcgaagatc attcccgaca
     8521 tcacctcggg caggctgaaa tacggaatcg acacgcaggt gccgggcatg atgtacgcgt
     8581 tgatcgcgcg ctgcccggtg ttcaagggca ggctcaggcg ttttgacgcg tcgaaggccc
     8641 tggccgtgaa aggcgtgcga aaagtgtttg cgatgacgcc ggtggcgggc catcacgagc
     8701 caccgtacat gccgcatgac atccgtgacg gcgtggccgt ggtcgccgat tcgttctggg
     8761 cggcgcgcaa gggccgcgat gcattgcaga tcgaatggga cgaaggggag aaggcgcgct
     8821 tcagcagcga ggatttcgcg cgtctcgccg cggatcgcgc gcgcgagcgc aaggacccca
     8881 cgggattcat cggcgacgac aacgcggtga acgatctcac gcgcgtccgc aagacgctgc
     8941 gtgcgtccta tatctatcca caccaggtgc actcgtgcat ggagccgctc aattgcacgg
     9001 ccttcgtcag gaaagacgac tgcgaagtgt ggtgcggatc gcaggcgccc aacctgatcg
     9061 tcggtgaact cgcgaaactg ctgaagctgc cgccggagca aatacgcgtc cacctgctcc
     9121 cgtccggcgg cggctttggc aggcgctact acccggactt cgccgtcgaa gctgcattca
     9181 tctcgcgcga ggccggcaac gtgcccgtga agatgatgtg gacgcgtgag gacgatcaga
     9241 ccgtcaacct cgcacatcac taccagcaca tggaatacca gacggccatc gacggcgcca
     9301 acaaactgtt cgcctggtac gagaaggaga tccgcaccta cacgtgggga tcacactacg
     9361 ccgggcccga actcccgcag atggcgtacg acatccccaa cattcgttat gacttcgagg
     9421 agctgaacga cgaggagttg gtccagtcat gcgcgtggcg aggggtcgtg agccacggcc
     9481 ggtcgttgag cgaatgtttt gtcgacgaga tcgccgccga gctcaagcgt gatccgctgg
     9541 agttccggct ttcactgctg acgcgtggcc gggacgcagt ggttagcgag ggcggcagga
     9601 tctcgtccga tcgcatgaca cgcgtgcttg cgctggcggc agagaaagcc ggttggggtg
     9661 gagagcttgc ggcgggacgc gggcgcggca tctgtctaag tccctatggc aacacctgcg
     9721 tggctgcgat cgcggaagtg accgtcaagg atggcgcgct caggatcgac cgcgtcaccg
     9781 tcgccgtgga ttgcggccgg ctcatcaacc cttccggcgc ggcgaaccag atcgaaggcg
     9841 gtatcgtgtg gagcctcacg ggcctgctct acggcggctt gccgatcaga aacgggcgag
     9901 cggtgaaccg gacattcgcc gagaacaaac tgttacgcat gaacgagtgt ccgaagatcg
     9961 acgtgcactt cgtgggcggc gatgccgagc ggccgtgggg aatcggcgag gtgtcgacgc
    10021 cggtgggcgc gccggcggtg ttgaatgcga ttttttcggc cacgggcaag cgcctgcgaa
    10081 cgctgccgat cgaaggccag agtctgacca gttagtccgg tgtgtagaca cgccgggtga
    10141 cgaagtagat cgcgagacca cctgccgtcg ccacgccgac atcgagcagc tcgcgcatcg
    10201 agctgttcgc gaggaatccc acgcacagca gcgcggatgc gactgcgacc agactgccgc
    10261 ccggcaatgt gaactgggcg gccggtgtgt tcggcatacg gcggaagata ggcaacgtca
    10321 cacaggtgat cgcgtagatc gaaagtcgcg cgatcagcgt gaccttgacc aggtagatga
    10381 acgaccccga gatcgcaagc gcgaggatcg acaccgcggt gaccaggatg ccgatgaccg
    10441 gtgtgcggta acgcttgtgc acgtatgcca gcagatgcgg caactggcgg tcgtctccca
    10501 tggaaaacag caggcgcgtg cccggcgtca tcgacgcgcc gaacacgccc gcgcaggaaa
    10561 tgacggcgac gatggcgatg accaccgcgc cggcgggtcc cacgagcagc aatgcgacgt
    10621 cggcgagcgg ccgctcggaa gccgcgaggt tcggcagcgt gccgacgcag acgacctgga
    10681 tcagtgtgta caacacgatg acgcaggcga ccgaaaacag gatggcgaac ggcaccgagc
    10741 ggcgcgggtc gcggacctcg cccgccagca ccgtggtgcc gtcgaagccc acgaacgcat
    10801 aggcggccag cagcagcgcg gtagataggt ccgtgatggc gggaagctct tcgagccgta
    10861 gcgcctgcgg ttcgacgaag agcaggccga tggccacgaa cgctaccagc ggcacgatct
    10921 tgagcaccgt gaacgtggtg ttgaccgcgg cggtctgacg tatgccacgg atgttgatcc
    10981 acgtgtaacc cagcaccagc agcgtgatag ccagcgcgcg cacacccggg tgcgagagcg
    11041 cgggccagag caccttgccg tagtccacgg cgaggttcga caccgcggcg caggagccga
    11101 tgcgcgagat ccacagcagc cagccgacgg cgaagcccgc cgtgggtccc aatgcctgcg
    11161 acgcgtagag ctgcggcccg ccggtgcgat cgaaccggct gccgacttcc gcgaagcaaa
    11221 gcgcgatcaa cgccatcagc aggccgcagg cgatgatgat gggcacgctg tagttgccga
    11281 ccagcgcctg gatcttcgcg ggcaggccga ggatgcccga gcctaccgtg agattgatgg
    11341 cgaacgccgt cagcgcccag cggccgatgc cgcggagaag gcggtcgtcg gcggtttggg
    11401 gtggtgaggt gggcacgcgc ggagtctacg cgtacgtacc ctggcgatca tccgaagggg
    11461 cgatgcgccg agcggagtga cgcacgcggc acaacggtga ttgaatatcc ccgccatgac
    11521 gcactcgcgg cgccaatttc tcgagatcag cctgctgagc ggcggcggcc tgctggtttc
    11581 gttcgcgctg cccgaattcg cgcgagcggc cactaacgaa cgccccggac catcctccgt
    11641 ccctctgggc gctttcgtac gcatcaacgc ggacaacagc atcgagatcg gcgcgcgcgg
    11701 ctgcgagatc gggcaaggcg tcatcacctc gctgcccatg ctcatcgccg aagagctcga
    11761 cgtgcgttgg gaccaggtga cggtgaagca actgccgtac ggaatcatgg cgggcaagga
    11821 accggggtcg ctcgctccca ggtatggcgg gcagggcgca ggcggcagta ccaacattac
    11881 cgacgccagg acggaattgc gcgaggccgg cgtgcaaatc cgccagttgt tagtcgccgc
    11941 ggccgcgcag ctgtggaccg ccgacgcggc cacgctcaag acccgcgacg cgatcgtctc
    12001 ccatcccgac ggccgcacgg ccacctacgg ttcactcgcg gcgcgcgcgg cgcagatgcc
    12061 gctgcccgcc gggcccttcg aactgcggaa gcccgccgac ttccgcatca tcggcaagcc
    12121 gacgaaaacc gcggactgct cggacattgt cgccggcgcg gcgcgctacg gcatcgacgc
    12181 gcaaatgccg ggcatgctgt acgcggtgat cgcgcgctgt ccgtacttcg aaggcacgct
    12241 gaaatccctc gacgacacgg cggcgaaaaa agtgcgcggc gtgcgcgccg tggtgcccat
    12301 cgcgccaccg ccagcgggcc cgctatcacg caatctcgcg gcaggcgtcg cggtgatcgc
    12361 ggacaacacc tgggcggcga tgcagggacg caaggcactg aagatagagt ggacgccggg
    12421 cccgtgggcc gccgattcca cgcaagctct ggaagcccgc tgccgcgctg ctgttgcggg
    12481 cgatgcgaac attcagaaag gccgcagcga cggcgacatg cttgccgcct gggcgggcgc
    12541 ggcacgcaag atcgaagccg actacaaggt gccgttcctc gcgcactcga cgatg
//
LOCUS       DATDEU010000115         4663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1480470, whole genome shotgun sequence.
ACCESSION   DATDEU010000115 DATDEU010000000
VERSION     DATDEU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480470"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..483
                     /locus_tag="VIV63_02820"
     CDS             79..483
                     /locus_tag="VIV63_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123559.1"
                     /translation="MNAVRARRRVRSLTCAAVLAALSFGVAAQNIFDFDDWMQRIDDG
                     SQNLQRHIAARDSQAARGAAREIEELYGLMEKFFEKRGNADDAVRWSREGREFAARAQ
                     ADIDARRFGSARRNALAIAHGCRDCHFSYKPL"
     gene            819..3533
                     /locus_tag="VIV63_02825"
     CDS             819..3533
                     /locus_tag="VIV63_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY6123560.1"
                     /translation="MSRSMPTAGSRTRLRWLLATLVVASAGASGVHAQTPGCQVTYTA
                     PTWVGGNGFGASIDIRNTGPQINGWSLVFAFPNGQRIQNGWPVTFTQPAGSPTVTVAS
                     NAPWNATINTNATFNVAFNGTFSGANNPPTAFTLNGVACNGGGNQNTAPTVSITAPTA
                     GQAFPPGTTSVNLAATAADPGGAVVRVEFRVDGVLVNTDTTSPYAFTATGLAAGAHTV
                     TATAVDNGSPALSTTTAATSFSIGSTGNTAPTVALTAPTNAQVFPVGTTTVNLAATAS
                     DSGGAVVRVEFRVDGTLRCTDTSSPYTCAATGLAAGNHTAQATAFDNGSPALSTATAL
                     VPFSITGTSNTAPTVSLTAPTSGQSFPAGSAVTLSANAADPGGAVIRVEFRVDGALVS
                     SDTTSPYSFSATGLAAGSHTATATAFDNGNPTLSTATAAVPFTIGATGAVFRVNPAGR
                     ITKNGTVFPVRCGSWFGLEGRHEPSDDPVNPSGAAMEQYIGNTSWVNGGQGSGRTIAQ
                     TMQEIAGMGINVIRLPLVPQTLNASDPQGTGNVLKNHSSVRIANSRLALETMIRAADA
                     ANIEVMLDIHSCSNYVGWRKGRLDARPPWTDADRDNYDFKRENYSCAATGNPSTVTTT
                     HPYNQTMWLDNLRTVAGLGTALGVDNIIGIDIYNEPHDYTWAEWKAMTEAAYAAINAV
                     NPNTLLFVQGVGTNAGAQDGSPTTITPVPHGVVATNPNWGENLFEAGTNPINIPKERL
                     VYSPHTYGPSVFVQTMFMDPAQTQCAGLEGDAAGDANCNIVINPTLLRQGWEEHFGYL
                     KQQNYAMVVGEFGGNMDWPLGQASVRDRDRWSHITPGIDTQWQNAFVDYMIEKGIEGC
                     YWSINPESGDTAGWYGHAYDPISNESGWGEWRAFDQRKTNLLNRLWGR"
     gene            complement(3589..>4663)
                     /locus_tag="VIV63_02830"
     CDS             complement(3589..>4663)
                     /locus_tag="VIV63_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY6123561.1"
                     /translation="LLFVLPGFVAIMALSIAYVLWHQQPVVEGMFLGLKAAVIALVIE
                     ALIRIGKRALTSNAALWVAGLAFVALAVLRLPFPLIVLGAGIAGYVRARLGLGSFAAA
                     RKNAASASAAPLPIIADSQLTQVRPSASRSFKLLALWLPLWFAPVVACAWWLGTASTY
                     TQLGVFFSKMAVVTFGGAYAVLTYVAQQAVERFAWVTPTQMLDGLGLAESTPGPLILV
                     VQFVGFLAAYQVAGGAHPLLAGALGAMLTVWVTFVPCFLWIFLGAPYIETLRNNVALA
                     SALAAITAAVVGVIANLALWFAVHVLFARIDEYGLPDLTSWRVVPALLTGFALLSMFV
                     LKWSLARTLAVSAVLGAISAMLR"
BASE COUNT          922 a         1510 c         1509 g          722 t
ORIGIN      
        1 cttcgatgct gcgtcgaatg gcgtgcgcgc agtggtcaag acctgcaagg cctgccacga
       61 agcctacaag ccacccgaat gaacgcggtg cgcgcgcgcc ggcgcgttcg ctctttgacc
      121 tgcgcggcgg tgctggcagc gctgtcgttc ggcgtcgcgg cgcaaaacat tttcgacttc
      181 gacgactgga tgcagcgcat cgatgacggc agccagaatc tccagcgcca catcgccgcg
      241 cgcgacagcc aggcggcacg cggcgcagcg cgtgaaatcg aagagttgta cggcctcatg
      301 gaaaagttct tcgaaaaacg cggcaacgcg gacgacgcgg tgcgctggtc gcgcgaaggc
      361 cgcgagttcg ccgcccgcgc gcaggcggac atcgacgcgc ggcgcttcgg gtccgcgcgc
      421 cgcaatgcgc tggcaatcgc gcacgggtgc cgcgattgcc atttcagcta caagccactg
      481 taggcgtcct gttagccaat cggcgagaca ggttagtcag acaacgagac aagttagcca
      541 aatcctgaga cagcgaaaac ccgccgacac tcgatacatg agtgttggcg acattcccct
      601 atggacagcg cacgggatgg gagtattttt gctgcgctca cagggggact tcgtttgcgc
      661 ttggtgccgc ctcatgcggc gcaattgatt catggagtga atctttccgt gcagggattg
      721 catgtgccgc gaattgtcac gcgcattccc tgcagctttc ttcccactcc tgccaacgca
      781 gacaagccat tggtatttcg ttgccaggag aatcaaacat gtctcgttct atgcctacgg
      841 ccgggtcccg gacccggttg cgatggttgt tagccacgct cgtcgtggct tcagcaggag
      901 cttcgggcgt ccacgcccag acacccggat gccaggtgac ctacacggcg ccgacctggg
      961 tgggtggaaa cggattcggt gccagcatcg acatccgcaa caccggacct cagatcaacg
     1021 gctggagcct ggtgttcgcc ttcccgaacg gccagcgcat ccagaacgga tggccggtca
     1081 cgttcacgca gcccgccgga agcccgacgg tcacggtggc gagcaatgcg ccgtggaacg
     1141 caaccatcaa caccaacgcg acattcaacg tcgccttcaa cggcacgttc agcggagcta
     1201 acaacccgcc gaccgcgttc acgctgaatg gcgtcgcctg caacggcggc ggcaaccaga
     1261 acacggcgcc cactgtatcc atcaccgcac ccaccgctgg ccaggccttc ccgcccggta
     1321 ccaccagcgt gaacctggcg gccaccgccg ccgatccggg tggagccgtg gtgcgcgtgg
     1381 agttccgtgt cgatggtgtt ctcgtgaaca ccgacaccac gtctccgtat gcgttcacgg
     1441 ccacgggtct tgccgccggc gcccataccg tcacggccac cgccgtcgac aatggcagcc
     1501 cggcgctaag caccaccacg gccgcgacct cgttctcgat cggctccacc ggcaacacgg
     1561 ctcccaccgt tgcgctgacg gcgccgacca atgcgcaggt cttccccgtg ggcaccacca
     1621 cggtgaatct cgcggcgacc gccagcgatt cgggtggcgc agtcgtgcgc gtggagttcc
     1681 gcgtcgacgg aacgttgcga tgcacggata cgtcgtcgcc ttacacctgt gcggctacgg
     1741 gtctcgcagc gggcaatcac actgcccagg ccacggcgtt cgacaacggc agtccggcgt
     1801 tgagcacggc gaccgccctg gtgcccttca gcatcacggg tacgtcgaat accgcaccca
     1861 cggtttcact caccgcgcct accagcggtc agtcgttccc ggcgggttcc gcggtcacgc
     1921 tgtccgcgaa tgcggccgat ccgggcggcg cggtcatccg cgtcgagttc cgggttgatg
     1981 gcgcactggt gtcgagcgat acgacctcgc cttacagctt cagcgccacg ggtctcgcgg
     2041 cgggaagcca cacggcgacg gcgaccgcgt tcgacaatgg caatccgacg ttgagcacgg
     2101 caaccgccgc ggtgccattc accatcggcg ccacgggtgc ggtgttccgc gtcaatccgg
     2161 ccggacgcat caccaagaac ggcacggtgt tcccggtacg ttgcggctcg tggttcggac
     2221 tcgaaggccg gcatgagccg tccgacgatc cggtcaaccc gagcggtgcg gcgatggagc
     2281 agtacatcgg caacacctcc tgggtgaacg gtggccaggg aagcggccgc acgatcgcgc
     2341 agaccatgca ggaaattgcc ggcatgggca tcaacgtgat ccgactgccg ctggttccgc
     2401 agacgctcaa cgcgagcgat ccgcagggca cgggcaacgt gctcaagaat cattcctcgg
     2461 tgcgtatcgc caattcgcgg ctcgcgctcg agacgatgat tcgcgcggcc gacgcggcca
     2521 acatcgaagt catgctcgac attcattcct gctcgaacta cgtgggctgg agaaagggtc
     2581 gtctcgacgc gcgtccacca tggacggatg cggatcgcga caactatgac ttcaagcgcg
     2641 agaactactc gtgcgcggcg accggcaatc cgtcgacggt gaccaccacg catccgtaca
     2701 accagacgat gtggctcgac aacctgcgca cggtggcggg tctgggcacg gcgctcggcg
     2761 tcgacaacat catcggcatc gacatctaca acgagccgca cgactacacg tgggcggagt
     2821 ggaaagcgat gaccgaggcg gcgtacgcgg cgatcaacgc cgtgaatccg aacacgctgc
     2881 tgttcgtgca gggtgtcgga acgaacgcgg gtgcgcagga cggcagcccg acgacgatca
     2941 cgccggtgcc gcatggtgtc gtggcgacca acccgaactg gggcgagaac ctgttcgagg
     3001 ccggcacgaa ccccatcaac attccgaagg agcggctggt ctactcgccg cacacctacg
     3061 gtccgtcggt gttcgtgcag acgatgttca tggatccggc gcagacacag tgcgcgggac
     3121 tcgaaggtga tgcggctggc gacgccaatt gcaacatcgt gatcaacccg actctgctgc
     3181 ggcagggctg ggaagaacac ttcgggtacc tcaagcagca gaactacgcg atggtggtgg
     3241 gcgaattcgg cggcaacatg gactggccgc tcggccaggc cagcgtccgc gaccgcgatc
     3301 gctggagcca catcacgccg ggcatcgaca cgcaatggca gaacgccttc gtcgattaca
     3361 tgatcgagaa gggcatcgag ggctgctact ggtcgatcaa tccggaatcc ggcgacaccg
     3421 ccgggtggta cggccatgcc tacgatccga tcagcaacga atcgggctgg ggcgagtggc
     3481 gtgctttcga tcagcggaag acgaatctgc tgaacagact ctggggtaga taggacgtct
     3541 ccccataggg ctcagatggc cgggacctga agtgaaacca ggtcccggtc atcggagcat
     3601 tgcggaaatt gcgccaagga cggcgctcac cgcgagcgtg cgcgcaagac tccacttgag
     3661 cacgaacatc gaaaggagcg cgaagccggt cagcagcgcg ggcacgacac gccatgatgt
     3721 gaggtcgggc agcccgtatt catcgatacg cgcgaacagc acgtgtacgg cgaaccataa
     3781 cgcgagattg gcgatgaccc cgaccacggc ggcggtgatc gcggcgaggg cgctggcgag
     3841 cgccacgttg tttcgcagcg tctcgatgta gggcgcgccg aggaagatcc acaggaagca
     3901 gggcacgaac gtgacccaga ccgtgagcat ggcgcccagc gctccggcca gcagcggatg
     3961 agcgccgccc gccacctggt acgcggccag gaagccgacg aattgcacga ccaggatcag
     4021 cggccccggc gtggattcgg ccagacccaa gccgtcgagc atttgcgtgg gcgtaaccca
     4081 ggcgaagcgt tccaccgcct gttgcgccac gtaggtgagc acggcatacg cgccgccgaa
     4141 cgtgaccacc gccatcttgc tgaagaacac gccgagctgc gtatatgtgc tggcggttcc
     4201 cagccaccac gcgcaggcca ccacaggcgc gaaccacaag ggcaaccaca gcgcgagtag
     4261 tttgaagctg cgcgacgcgg agggccggac ctgcgtgagc tgtgagtcgg cgatgatggg
     4321 cagtggcgcg gcagaggcgg aagccgcgtt cttgcgcgca gcggcgaatg agccaaggcc
     4381 caggcgggca cgaacgtacc ccgcgattcc cgcgcccagc acgatgagcg ggaagggcag
     4441 ccgcaggact gcgagtgcga cgaacgcaag ccctgccacc catagtgcgg cgttgctcgt
     4501 gagcgcgcgt ttgccgatgc gtatgagcgc ttcgatcacg agtgcgatga ccgcggcctt
     4561 gagtccgagg aacatgcctt cgacgacggg ctgctggtgc cagagaacgt acgcgatgga
     4621 cagcgccatg atcgcgacga agcccggcag cacgaacagc aga
//
LOCUS       DATDEU010000116         3205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1918118, whole genome shotgun sequence.
ACCESSION   DATDEU010000116 DATDEU010000000
VERSION     DATDEU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918118"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..578)
                     /locus_tag="VIV63_02835"
     CDS             complement(33..578)
                     /locus_tag="VIV63_02835"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6123562.1"
                     /translation="MTACLLVVPKAAPLARFAALALAFAFGLAGVAATAAEKHELLGQ
                     PAPDLVARGLSGENVRLSEHRGEVVVVSFWSGTCNTCRAQLEALDRIAKTYGSAGLTV
                     IGVNLDDNPLRAEKFARAQDVDFQLLVATAKSTGRDFRVDRLPMILFVDRSGVLRVAH
                     REFKSRDEAIYVRELRTLLDE"
     gene            complement(807..2414)
                     /locus_tag="VIV63_02840"
     CDS             complement(807..2414)
                     /locus_tag="VIV63_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123563.1"
                     /translation="MYILKKSPRTLGLDEPIKHDSHKAPVSRRDFIAQGFRTGPAIVA
                     GGAILSALMGRPARGAMSQPLEDFAADICNISAGAGKVPFICFDLAGGANLNGSEILV
                     GAGDPLNFLSTQGYATLGLPGNMTPGSANAASPTNNFVNTDFGAPWHSDGAMLRGMLM
                     STQAATRANTNGFGIAARSENDTGNNPHNPMYGINRIGADGELLTLIGSQNTDSGGNS
                     MAPASLMNPAARPTKVDRASDVTGLVDTGELSTMFAESNDAIAVLESMTRLGHRKTNL
                     IDPKLASAGQEEVLKELVKCGYVKSTYLADRFRSPAALNPNLDADIVGPTGIFTDAEY
                     NADGEFRKTAAVMKMVINGYAGAGTVTMGGYDYHGQGRNTGENRNFRAGRCIGACLEY
                     ARRMNKPLMIYVFSDGSLSANGVPNMTAEGRGKLDWASDNQSTACSIVLVHRPAGRVT
                     FRSDTRKQIGSMSMGGAVNTASSPAANAVNLLVETVLLNYMALHGEHGNFDQPAFFPR
                     HGLGGTAARDALTIFNPVCNGTVSNPV"
     gene            complement(2426..>3205)
                     /locus_tag="VIV63_02845"
     CDS             complement(2426..>3205)
                     /locus_tag="VIV63_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123564.1"
                     /translation="EQHAGQAYVPLSTTIGGTSYTAGAGQLMSSVGTVMAIDKTVVDD
                     VFFLSFERIGALSHTPAEPPLPTPATPADLEPESDVGLRTFDELNATMSQITGVPQTN
                     VRVRDTFSLVRQALPSIERFGAFGPAQQTALAQLAMQYCNVMVDDNALRAAFFGGLDG
                     SGTGTAVFGTAGAPNTANRDLLINALIDKAVGGGMSFQVTDAQLRGELNGIDDPDNTF
                     DTAGLINRLVSGPTGSSSTGGRTVMKAACGAVLGSGATLIQ"
BASE COUNT          535 a         1000 c         1102 g          568 t
ORIGIN      
        1 gggtggttgg cggcataaag gttctcgtcg gattattcgt ctagaagcgt gcgcaactcg
       61 cgcacgtata tggcttcgtc acgggacttg aactcgcggt gcgcaacacg cagcaccccc
      121 gagcgatcca cgaacaggat catgggcagc cggtcgacgc ggaagtcgcg gccggtgctc
      181 ttggcggtgg ccacgagcaa ctggaaatcg acgtcctgcg ctcgcgcgaa tttctcggcg
      241 cgcagcgggt tgtcatcgag attgacgccg atgaccgtga ggccggcgct gccgtaggtc
      301 ttggcgatgc ggtcgagcgc ttcgagctgc gcgcgacagg tgttgcacgt gccgctccag
      361 aaactaacta ccaccacttc gccgcggtgt tcggagagtc gcacgttctc gccggagagg
      421 ccgcgcgcca ccaggtcggg tgccggctgg ccgagcagct cgtgtttttc ggcggccgtg
      481 gccgcgacgc cggccaaccc gaaggcgaac gccagcgcaa gcgcagcaaa acgcgcgagc
      541 ggcgcggcct tgggcacgac taacaaacaa gcggtcacgg ttggctcaaa gtaagaaaac
      601 accgggcaag cgatgcggac aagcgacgcc ggaaaccttc ccatagccca cttaacaatg
      661 tcaacgaagc ggagccccgt ttgagaagtc gatcgtggcg aagattgcgg cacgtccggg
      721 gtcgcacaaa aggcaaagga gcgtcggcgc cgcgctgtcg cgtgttgccg acgctccctt
      781 gaggtcgata cggctcgagc tcgcgctcag accgggttgc tgacagtacc gttacagacc
      841 gggttgaaga tcgtcagggc gtcgcgcgcc gcggtgccac ccaggccatg cctcgggaag
      901 aacgcgggct gatcgaaatt gccgtgttcg ccgtgaagcg ccatgtagtt gagcaacacc
      961 gtttccacga gcaggttgac ggcgttcgca gccgggctgg acgcggtgtt caccgcgcca
     1021 cccatgctca tcgagccgat ctgcttgcgc gtatcgctgc ggaaggtcac gcggcccgcc
     1081 gggcggtgca cgagcacgat cgagcaggcc gtcgactggt tgtcgctggc ccaatcgagc
     1141 ttgccacggc cctcggccgt catgttcggc acgccgttcg ccgacagcga gccgtcgctg
     1201 aacacgtaga tcatgagcgg cttgttcatg cggcgcgcgt actcgaggca ggcgccgatg
     1261 cagcggcccg cgcggaagtt gcggttttcg ccggtgttgc gtccctggcc gtggtagtcg
     1321 taaccaccca tcgtgacggt gccggcgccc gcgtacccgt tgatgaccat cttcatgacg
     1381 gccgcggtct tgcggaattc accatccgcg ttgtactccg cgtcggtgaa gatgccggtg
     1441 gggccgacga tgtcggcatc gagattcgga ttgagcgccg ccggcgagcg gaagcggtcc
     1501 gccaggtagg tggacttcac gtagccgcac ttcacgagtt ctttgagcac ctcttcctgg
     1561 cccgcgctcg cgagcttcgg atcgatgagg ttggtcttgc gatggcccag gcgggtcatg
     1621 gattcgagca ccgcgatggc gtcgttcgat tcggcgaaca tcgtcgagag ttcgccggtg
     1681 tcgacaagac ccgtgacgtc gctggcgcga tccactttcg tgggacgcgc ggccggattc
     1741 atgagtgatg ccggcgccat cgagttgccg cccgaatcgg tgttctgcga accgatcagc
     1801 gtcagcagtt cgccgtcggc gccgatgcgg ttgatgccgt acatcgggtt gtgcgggttg
     1861 ttgcccgtgt cgttctccga acgggccgcg atgccgaagc cgttcgtgtt ggcacgcgtg
     1921 gcggcctgcg tcgacatcag catgccgcgc agcatggcgc cgtcggaatg ccacggagca
     1981 ccgaagtcgg tgttcacgaa gttgttagtc gggctggccg cattggccga acccggcgtc
     2041 atgttgcccg gcaggccgag cgtggcgtag ccctgcgtgg acaggaagtt cagcggatcg
     2101 cccgcaccca ccaggatttc cgaaccgttg aggttcgcgc caccggcgag gtcgaagcag
     2161 atgaaaggca ctttgcccgc accggcggag atgttgcaga tgtccgcggc gaagtcctcg
     2221 agcggctggc tcatcgcacc gcgggccgga cgacccatca gcgcgctgag gatcgcacca
     2281 ccggcgacga tggcaggtcc cgtgcggaaa ccctgggcga tgaaatcgcg ccgagacacg
     2341 ggagccttgt ggctatcgtg tttgatgggc tcgtccagtc ccagtgtgcg cgggctcttc
     2401 ttgaggatgt acatgctttc tctccctact gaatcagcgt cgcgccgctg ccgaggacgg
     2461 caccgcacgc tgctttcatg accgtacggc cgcccgtcga gctagagccc gtggggccgg
     2521 ataccaggcg attgatgaga ccggcggtgt cgaaggtgtt gtccgggtca tcgatgccgt
     2581 tcaactcgcc gcgcagctgc gcgtcagtga cctggaagct catgccgccg cccaccgcct
     2641 tgtcgatgag cgcattgatc agcaggtcac gattggccgt gttcggggcg cccgccgtgc
     2701 cgaacaccgc ggtgccggtg cccgagccgt cgagcccgcc gaagaacgcg gcgcgcagag
     2761 cgttgtcgtc gaccatcaca ttgcagtact gcatcgcgag ctgcgcgagc gccgtctgct
     2821 gtgcggggcc gaaggcaccg aaccgctcga tggacggcag tgcctgccgg accagcgaga
     2881 aggtgtcgcg cacgcggacg ttggtctgcg gcacaccggt gatctgcgac atcgtcgcgt
     2941 tgagctcatc gaaggtgcgc aggcccacgt cggactcggg ttcgagatcc gcgggcgtgg
     3001 ccggcgtcgg cagcggcggt tcagccggcg tgtggctgag cgcaccgatg cgctcgaagg
     3061 acaggaagaa cacgtcgtcg acgacggtct tgtcgatcgc catgacggtg ccgacgctcg
     3121 acatcagctg gcccgcgccg gcggtgtagc tagtgccacc gatggtcgtg ctcagcggaa
     3181 cgtacgcctg gccggcatgc tgttc
//
LOCUS       DATDEU010000117        11850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_751594, whole genome shotgun sequence.
ACCESSION   DATDEU010000117 DATDEU010000000
VERSION     DATDEU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751594"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VIV63_02850"
     CDS             <1..402
                     /locus_tag="VIV63_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6123565.1"
                     /translation="ASRQGFEAEDLDHFKGLHKRDPLLALVMLALMFSTAGVPPFIGF
                     WAKFNIFQALWLTGHYWLILIAAAASVVGVFYYLRIVKLMYFDAPGELPAGPSSGMAV
                     RGVLALNGLAVLALGIVPNALIQLCARVIGY"
     gene            476..552
                     /locus_tag="VIV63_02855"
     tRNA            476..552
                     /locus_tag="VIV63_02855"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:510..512,aa:Met,seq:cat)
     gene            907..1449
                     /locus_tag="VIV63_02860"
     CDS             907..1449
                     /locus_tag="VIV63_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123566.1"
                     /translation="MSIARHVIAIAALFTMSSAPALAENPVALQREYLATKGQFVLVA
                     PAHMNLADVHARLITLMNDSGSELQEVDAVTITARTTFATFADPMLVPILGGVVDLNY
                     QRAVNDAAGVPIYQVRRITLTQEDGQPVRVQFCSRVKQYTPRSNGAPDVRVATQCKLA
                     FREWNALREATSRLFESPPV"
     gene            1603..1935
                     /locus_tag="VIV63_02865"
     CDS             1603..1935
                     /locus_tag="VIV63_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123567.1"
                     /translation="MARIVVFRSVVLAAGVLLIGACASQPTSSLDEKYFQKEASNYLK
                     FQHEGQTVYCRTEEGAPSLIPFNPNKRCITETALRQAVENHRINRNAVAPAPGAGSGQ
                     GYLGWSGR"
     gene            2003..2344
                     /locus_tag="VIV63_02870"
     CDS             2003..2344
                     /locus_tag="VIV63_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123568.1"
                     /translation="MARNLIVRSVILSTVALLIAACASQPASSVAYASAQSSRLDKKF
                     QATARQYQKFRHEGQTMYCKKEKVITSAIPVMQCLSEPQLRLQVENYERSRNPIARPL
                     APGAGQGGIGG"
     gene            complement(2399..2989)
                     /locus_tag="VIV63_02875"
     CDS             complement(2399..2989)
                     /locus_tag="VIV63_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY6123569.1"
                     /translation="MARHVSDPFVKKAQLEGYRSRSAYKLIELNEKDRLIRPAMRIMD
                     LGSAPGGWSQVAGKLVGAKGRVLATDILPMEPIKNVDFVQGDFTDQKVVDELLTWLGG
                     GKFDLIISDIAPNITGIDSADQASSMYFLDLALDTVRQTLKPGATFVAKMFQGSGSDD
                     YVKELRTSFEKVLIRKPAASRAQSREVYIVAKGFKA"
     gene            3092..4696
                     /locus_tag="VIV63_02880"
     CDS             3092..4696
                     /locus_tag="VIV63_02880"
                     /inference="COORDINATES: protein motif:HMM:NF044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6123570.1"
                     /translation="MIRITELPLPLDYTAEALRQAVVKRLRIRDADLLEHTLFKRSYD
                     ARKKNTGILFICIVDVKLADEAAVLKRLARDRQIAPAPDTAYHPVAKAPATLSARPLV
                     IGFGPGGLFAALLLAQMGFKPIVLERGRDVRRRTQDTWGLWRKKILTPESNVQFGEGG
                     AGLFSDGKLYSQIKDPKFYGRKVMHEFVRAGAPAEILFVSKPHIGTFRLTGIVEKMRA
                     EIIELGGEVRFESKVTDLLIDNGQVEGAVLENGETLHSRHVVLALGHSSRDTLRMLER
                     RGVFLEAKPFAIGFRIEHPQSLIDRARFGKFAGHPELGAADYKIVHHASNGRSVYSFC
                     MCPGGMVVAATSEPGRVVTNGMSQYSRNERNANAGIVVAINPEGDFPGGPLAGVALQE
                     ALETKAFELGGGDYCAPGQLVGDFVRGNASTQLGEVQPSYQPGVLLGDLGPSLPAYAI
                     DAIREALPAFGRQIHGFDRDDAVLTGVESRTSSPVRITRDAESLQSLNVRGLYPCGEG
                     AGYAGGILSAGVDGIKVAEAVAREMA"
     gene            4693..5415
                     /locus_tag="VIV63_02885"
     CDS             4693..5415
                     /locus_tag="VIV63_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY6123571.1"
                     /translation="MKHEDIKKLHQKKFREELGHFVIEGEHLVQELEKAAVRDARLRQ
                     AEIYVTRDYAHWSSTLPMHVIQSKQMAQISETRSPQGIAAVVPLLTPAAPRPGERAIY
                     LHEIQDPGNLGTILRTLAWFGNFRCLTSADSVDVHNGKVVRASMGAIFHVPVELDVPL
                     DALQNRFARIASLDLSGQPVASAAFREFDCYVFGTEARGLPREQMHALGARAFTIPGT
                     GAIESLNVAATVNICLYELNRG"
     gene            5479..6531
                     /locus_tag="VIV63_02890"
     CDS             5479..6531
                     /locus_tag="VIV63_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123572.1"
                     /translation="MAVLLPGLLALAGCASIGPATVARDRFDYVESISDSWKRQMLLN
                     LVKVRYADAPVFLDITSVISSYEFRGELGVFGQEAPAGRAGDSFIGGAVNGSYVDRPT
                     ITYAPLSSDKFAKALMSPFPITGLGLLLQSGYPADAVLRICANSINGLSNRYGGRGAR
                     PGDRRYPELLTLLREAQNRGDIALYSEKVYDQHVVKLGLRPTADATATARNRRIADLL
                     ELDGVTGEFNVVYGARPASHKEIAIQSRSMMQVLIDLGSYIEVPARDIAEGRVFVAPR
                     DEELDPLFTALMRVHSSDSAPKDSHVAVRYRDGWFFIDDRDHESKAAFNFLMLLFSLT
                     ETGTTQAAPIVTVPAR"
     gene            6609..9584
                     /locus_tag="VIV63_02895"
     CDS             6609..9584
                     /locus_tag="VIV63_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY6123573.1"
                     /translation="MSTADSGDALKRDALAALDVLLEKPPAERTELLGALAQSRPELH
                     TLVIELLAYEARLNEGFMEPGASRISQESLHVGSHLGPYRIERLLGEGGMGEVWLATR
                     DDGLYDGQVAIKTLHPYFAGGAMRERFLREARVLGRLAHPNIARMLDAGVHEGVVYLV
                     LEYVVGRQIDRACDDESLDVSARLEIFLQLCAAVAHAHSSLVVHRDIKPGNVLLTAGN
                     VPKLLDFGIASFFEPEKGEAQPAITRLTGRIFTPEYAAPEQVLGQEITTSTDVYSLGV
                     LLYVLLAGRLPYAPAEGGRTHWEHAVLHEEPQRMARALDPAGQTALASSRSMPYARLR
                     RELGGDLEVIVQKALRKRPEDRYLTVAAFADDIRRYLQGEPVLARADSSWYRLRKFAR
                     RNRLAVVSTLAVVLALGIGFAVSLWQLQVAHAERQRAEESKEFIASIFRSADPFYTGK
                     ESMTAAELLTLARERIDSELVAQPQNAVELLTLVGESQLNLSQDAAAKATLTKAVEMA
                     ERLQPRDDVLIAEARAQMAGVASENGEWEQVRAMAALALPDLRKYQPRTARLLNGMLI
                     SLAYAESDDGNREAAIALSREGVAAVTAALGPMHSETISGRYHLATLIQQAGRLDEAR
                     AVAEQVLRDAHALASDGHSGALVIQAEGRYGTVLLEMGELQSAISHLKAANDLAAKLY
                     GPKNPSRYNWFSSLARAQQRLGDFKGLLVTRRQAYEGNQEGGISQARDLTNLARSTFA
                     ARKVQDSVDLLRRAIELEKQYDVGKGSWIVLAQSDYGAALALSGQLEQADRELETALA
                     FAKESAKEDSLESTWNAIGLARQVRSQWAESEQAFREALASTTSTAINQKFRAEALQG
                     IGVARLERGDAADGENWLRQADESVRKMFVGMTPLRADIAMNLGRTLLAQRKIEAANE
                     SFTAANSYWLDYDESNRSAGIAAYWLAQGHLAAAASSEARIELERSVGILEHSPLPGD
                     AKLVQAARRDLVRLPKQGS"
     gene            complement(9581..10249)
                     /locus_tag="VIV63_02900"
     CDS             complement(9581..10249)
                     /locus_tag="VIV63_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6123574.1"
                     /translation="MKALETVTVETGPNPTFSIIWMHGLGADGHDFEPLVPELLDAGM
                     PVLRFVFPHAPVRPVTINNGYQMRAWYDIIGIDRRSAEDFDGINASAKAVGALIKAEN
                     DRGVPSNRIAIAGFSQGGAMALHIATRHTEKLAGVIALSCYLPLARELAAARNPANQS
                     TPIFMAHGTQDPVVPYPLGEDSHRLLKDAGYPVEWHAYPMPHSLCEPEVTDIRAWLKR
                     VSAN"
     gene            complement(10246..11358)
                     /locus_tag="VIV63_02905"
     CDS             complement(10246..11358)
                     /locus_tag="VIV63_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY6123575.1"
                     /translation="MQKVVTISLNGNSYQLEEPGFNLLRSYLDRAETRLKDSPDRGEV
                     LGDLEQAIGEKCRGMLGPHKSVVSTTEVERIIAEMGPVESADEKAASAASDGGTANAE
                     PASFGPEPRKRLFKIREGAMWGGVCNGLAAYMGLDVTWVRIGTVVLTLFTGVTLLIYL
                     AMLIIVPYADTAEDRAAAFGVPFNTEDIINRAKKNFDETNHRWHREWRRQQRHMSRQF
                     RHMNETLRQTAANVAPNMSPAARGIMAVFVPVAAIIGAVIFVGWILAMASLVTQHTIF
                     GWGLPHGVPLWVGILGLALLYFMISTPFKMIRNGGHQAAGHHPGWGALHGFMWIGFTV
                     LFFWLAYTFFPGVRELLDQLMWAANLTASNISETIV"
     gene            complement(11362..11685)
                     /locus_tag="VIV63_02910"
     CDS             complement(11362..11685)
                     /locus_tag="VIV63_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY6123576.1"
                     /translation="MNISEKLAPIRKGLLEFVILKIVAGDKVYVADMLKLLGPTEFAT
                     QEGTLYPLLSKLRREELVDYEWQESEAGPPRKYYKLTQKGRTQLSTLNDYWKELNRTL
                     ADFGN"
BASE COUNT         2172 a         3702 c         3813 g         2163 t
ORIGIN      
        1 gccagccgcc agggcttcga agccgaagac ctcgatcact tcaagggact gcacaagcgc
       61 gatccgctgc tcgcgttggt catgctcgcg ctgatgttct ccaccgccgg cgtgccgccg
      121 ttcatcggct tctgggcgaa gttcaacatc ttccaggcgc tgtggctgac gggtcactac
      181 tggctgatcc tgatcgccgc cgctgcgtcc gtggtcggcg tgttctatta cctgcgcatc
      241 gtgaagctca tgtatttcga cgcgccgggc gagctgcccg caggcccgtc gagcggcatg
      301 gccgtgcgcg gcgtgctggc actcaacggc ctggcggtgc tggctctcgg aatcgtgccc
      361 aacgcgttga ttcagctctg cgcccgcgtg atcggctact gagattcagt taataaggtt
      421 tgcaaaggcg cacctcttgg ataagaatgc gcgcccctcg ccagagggga tcggttgcgg
      481 ggtggagcag tctggcagct cgtcgggctc ataacccgaa ggtcgtaggt tcaaatccta
      541 cccccgctac cacttataca aaggcctcgg cgcaaaacgc cggggccttt ttcgttcgag
      601 cggccccaac gtcttgcacg tcgacaaccc gatcgatcgt tacccgtggg ccggccgtga
      661 cgggatgaaa cacggcaaag acggctcact gactatctac attcaacgcc agccccctgg
      721 acgcgagtca tcgcttctcg ttcgcccctg gctctctagt tagtgcaaag agtaggggtg
      781 cttccccttc cgggttttcc tccattgcgg aactgtgtgg cgcgtcgcgt gcgaacctca
      841 ttgatcaaag cagttgcgcc ctaaaatcgt ctccctcatt gggtcgacca tcaggaagca
      901 ggagatatgt cgattgccag acacgtgatc gcaattgccg cattgttcac catgagcagc
      961 gccccggcac tcgctgagaa tccggtcgca ctgcaacgcg aatacctggc gaccaagggc
     1021 cagttcgtgc tggtcgctcc agcgcacatg aacctcgctg acgtgcatgc gcgcctgata
     1081 acgctcatga acgacagcgg aagtgaactg caggaagtcg acgcggtcac gatcaccgca
     1141 cgcacgacct tcgctacatt cgctgacccg atgctagtgc cgattctggg aggggtggtc
     1201 gatctcaact atcaacgcgc ggtgaacgac gccgcgggcg tacccatcta tcaggtgcgg
     1261 agaatcacgc tgacgcagga agacggccaa cccgtgcgcg tgcagttctg ctcgcgagtc
     1321 aagcagtaca cgccgcgcag caacggcgcg cctgacgtac gggttgcgac gcagtgcaaa
     1381 ttggcgttcc gcgagtggaa cgcgctgcgc gaagcgacgt cgcggctctt cgagtcacca
     1441 ccagtctgaa ttgaacgccc gcattgggct gggcgtcttc aggacggcgc ggacaggcag
     1501 gttccgaagc gataagtcgc gaccgccttg ctagattccg tctaacggag taaatcgtca
     1561 tttcaatgcg ctcctggcgc gtccgcctgt tcggtgaacg tcatggcaag aatcgtggtt
     1621 ttccgatccg tcgttctggc cgccggagtg ctcctgattg gcgcttgcgc ctcgcaaccc
     1681 acttcatctc tggacgaaaa gtatttccag aaagaagcca gcaactacct caagttccag
     1741 catgaagggc agacggtgta ctgccggact gaagagggtg cgcccagcct gattcccttc
     1801 aacccgaata agcggtgcat caccgagacc gcgcttcgtc aggcagtcga aaaccatcgg
     1861 atcaaccgca acgccgtagc gcctgcgcca ggggcgggct ccggtcaggg gtatttaggg
     1921 tggagcggac gttagataac gtcccactga gtaaacggta attcagcgcg cttccagcgc
     1981 gtccattgac cgggtcaatg acatggcaag aaacctgatt gttcgttccg tcattctgtc
     2041 caccgtggct ctcctgatcg ccgcgtgcgc ctcgcaaccc gcttcctcgg tcgcttacgc
     2101 atcggctcaa tcgtcgcggc tcgacaagaa gttccaggca accgccaggc agtatcagaa
     2161 gttccggcat gaaggccaga cgatgtactg caagaaggaa aaggtcatca ccagtgccat
     2221 cccggtcatg cagtgcctga gcgagccgca gcttcggctg caggtcgaga actatgaaag
     2281 gtcgcgcaac cccatagcgc gtcccctggc gccaggtgcc ggccaaggtg gaatcggggg
     2341 ctgattcgcc tgttcgagtt acgccacgcc cgaatgaaga tttcgagcta gatgcgggtc
     2401 aagccttgaa tcccttcgcg acgatgtaga cctcgcgcga ttgcgcgcgt gacgcagcag
     2461 gctttctgat cagcaccttc tcgaacgacg tgcgcagctc cttcacgtag tcatccgatc
     2521 ccgatccctg gaacatcttc gccacgaacg tcgcgccggg cttcaaagtc tgacgcacgg
     2581 tgtcgagcgc gagatccagg aaatacatcg acgaagcctg gtccgcggaa tcgatgccgg
     2641 tgatgttggg cgcgatgtcc gagatgatga gatcgaactt tccgccgcct aaccacgtga
     2701 gcagttcgtc gacgaccttc tggtcggtga agtccccttg cacgaaatcc acgttcttga
     2761 tgggctccat gggcaggatg tccgtggcga gcacgcggcc cttggcgccc acgagtttgc
     2821 cggcgacctg cgaccacccg ccgggcgcgg agccgagatc catgatccgc atcgccgggc
     2881 gaatgaggcg atccttctcg ttgagctcga tgagcttgta ggcggatcgc gaccggtaac
     2941 cttccagctg cgccttcttc acgaaggggt cgctgacgtg tcgcgccatc caggttgagc
     3001 tactctttga tcggggcatt tttaacttct ggcgctagaa tgccgcgcat tctatctcta
     3061 cctctgcccg gacacgggcc aggaagtcgc agtgatccgc attaccgagc tgccgctgcc
     3121 gctcgactac accgccgagg cgctgcgcca ggccgtcgtc aaacgcttga ggatccgcga
     3181 cgcggatctg ctcgagcaca cgctgttcaa gcgtagctac gatgcgcgca agaagaacac
     3241 cggcatcctg ttcatctgca tcgtcgacgt gaaactggcc gacgaagcgg cggtgctgaa
     3301 gcgactggcg cgcgatcgcc agatcgcccc cgcacccgac acggcctatc acccggtcgc
     3361 gaaagcgcca gcgacgctct cagcgcggcc tctggtcatc ggattcgggc ccggtggatt
     3421 gtttgccgcg ctgctgctcg cgcagatggg attcaagccg atcgtgctcg agcgcggccg
     3481 cgacgtgcgc cggcgcaccc aggacacctg gggcctgtgg cgcaagaaga tcctgacgcc
     3541 cgaatccaac gtgcagttcg gcgagggcgg ggcgggcctg ttctccgacg gcaagctcta
     3601 tagccagatc aaggatccga agttctacgg ccgcaaggtc atgcacgagt tcgtgcgcgc
     3661 gggcgctccc gcggagatcc tgttcgtcag caagccgcac atcggcacgt tccggctcac
     3721 cggcatcgtc gagaagatgc gtgcggaaat catcgagctc ggcggcgagg tgcgtttcga
     3781 gagcaaggtg acggacctgt tgatcgacaa cggccaggtc gaaggcgcgg tgctcgaaaa
     3841 cggcgagacg ctgcacagcc gccacgtggt gctcgcgctc ggccacagct cgcgcgacac
     3901 gctgcgcatg ctcgagcggc gcggcgtgtt cctcgaggcc aagccttttg cgatcggctt
     3961 ccgcatcgag catccgcaat cgctcatcga ccgcgcgcga ttcggcaaat tcgcgggtca
     4021 tccggagctg ggcgccgcgg attacaagat cgtgcatcac gcgagcaatg ggcgttcggt
     4081 gtacagcttc tgcatgtgcc cgggcggcat ggtggtggcg gcgacgtcgg agcccgggcg
     4141 cgtcgtgacc aacggcatga gccagtactc gcgcaacgaa cgcaacgcca atgcgggcat
     4201 cgtcgttgcc atcaacccgg agggagattt tccgggcggg ccactcgccg gtgtcgcact
     4261 gcaggaggcg ctcgagacca aggctttcga gctgggtggt ggcgactact gcgcgccagg
     4321 acaactggtg ggcgatttcg tgcgcgggaa tgcctccacg cagttgggcg aggtccagcc
     4381 ctcgtaccag ccgggcgtgt tgttaggcga cctcggtcct tcgctgccgg cctacgccat
     4441 cgacgccatt cgcgaagcgt tgcccgcgtt cggcaggcag atccacggtt tcgatcgcga
     4501 cgatgcggtg ctcaccggag tcgagagccg cacctcctcg ccggtgcgca tcacgcgcga
     4561 cgcggaatca ctgcagagtc tcaatgtgcg cgggctgtac ccttgcgggg aaggcgcggg
     4621 atacgccggc ggcatcctgt ccgcgggtgt cgatggcatc aaggtggccg aggcggtcgc
     4681 gcgggagatg gcttgaaaca cgaagacatc aagaagctgc accagaagaa atttcgcgag
     4741 gagctcgggc acttcgtcat cgaaggcgaa catctcgtgc aggaactcga gaaggccgcg
     4801 gtgcgcgatg ccagactgcg gcaggccgag atctatgtga cgcgcgacta tgctcactgg
     4861 tcgagcacgc tgccgatgca cgtgatccag tcgaagcaga tggcgcagat cagcgagacg
     4921 cgttcgccgc aaggcatcgc cgcggtcgtc ccattgctga cgccggccgc gccacggccg
     4981 ggggagcgcg cgatctatct tcacgagatc caggatcccg gcaacctcgg gaccatcctg
     5041 cgcacgctgg cgtggttcgg aaatttccgc tgtctcacca gcgccgacag cgtcgacgtg
     5101 cacaacggca aggtggtgcg cgccagcatg ggcgccatct tccacgtgcc cgtcgagctc
     5161 gatgtcccgc tggatgcgtt gcaaaaccgg ttcgcgcgca tcgcgtcgct ggatctcagc
     5221 gggcagccag tcgcatccgc cgcattccgg gagttcgact gctacgtgtt cggtaccgaa
     5281 gctcgcggcc tgccacgcga gcaaatgcac gcgctcggcg ccagggcctt caccattccc
     5341 gggaccggcg ccatcgaatc gctgaacgtg gcggcgaccg tgaacatctg cctgtacgag
     5401 ctcaatcgcg gctagggtac tttgggccta ttaccccgac agcagccggc tcctaatatc
     5461 ccggtcgaca tgaaacgcat ggctgtgctg ctcccgggcc tgctggcact cgccggctgc
     5521 gcgagcattg gtccggctac ggtggcgcgc gaccgcttcg actacgtcga atccatttcc
     5581 gactcgtgga agcggcagat gctgctgaac ctcgtcaagg ttcgttacgc cgacgcgccg
     5641 gtgtttctcg atatcacctc ggtgatcagt tcgtacgagt tccgcgggga actgggtgtc
     5701 ttcggtcagg aggctccagc cggccgcgcg ggagattcgt tcattggtgg cgccgtgaac
     5761 gggtcgtacg tcgaccgtcc cacgatcacc tacgcgccgc tctcgagcga caagtttgcc
     5821 aaggcgttga tgtcgccttt cccgatcacg ggcctcggac tgctgctgca gtcgggttat
     5881 ccggcggatg ccgtactgcg aatctgcgcg aattcgatca acgggctcag caaccggtat
     5941 ggcggccgcg gagcgcgtcc cggtgaccgg agatatccgg aactcctgac gttgttgcgc
     6001 gaagcgcaga accggggcga catcgccctc tattcggaga aggtgtacga ccagcacgtg
     6061 gtgaagctgg gcctgcgacc cacggcagac gcgacggcca cggcgcgtaa ccgccgcatc
     6121 gccgacctgc tcgaactcga cggcgtcacc ggagaattca atgtggtcta cggcgcgcgc
     6181 ccggcgagtc acaaggaaat cgcgatacaa agccgctcga tgatgcaggt cctcatcgat
     6241 ctgggttcgt acatcgaggt gccggcgcgt gacatagccg aaggccgcgt cttcgtcgcg
     6301 ccgcgcgacg aagagctgga ccccttgttt acagcgctga tgcgcgttca ttcatctgat
     6361 tcggcaccga aagactcgca tgtagcggtg cgttatcgcg acggatggtt cttcatcgac
     6421 gaccgcgatc acgaatccaa ggccgcgttc aatttcctga tgttgctgtt ctcgctcacc
     6481 gagaccggca cgacgcaagc cgcgccgatc gtcaccgtgc cggcgagata aacgtaccgg
     6541 cgcatttcgc gccaacgccg cgcgacgcta tcatttcgcc cacccaggcg aaaaagcacg
     6601 gacgagccat gagtacagcg gattccggcg atgccctgaa gcgtgatgcc ctggccgcgc
     6661 tggatgtact cctcgagaaa ccgcctgcgg agcgcacgga attgctcggc gcattggcgc
     6721 agtcccggcc ggagctgcat acgctggtca tcgaactgct ggcgtatgaa gcgcgcctca
     6781 acgaggggtt catggaacct ggcgcttccc ggatcagcca ggaaagtctg cacgtcggca
     6841 gccacctcgg cccctaccgt atcgagcggc tgctcggcga gggcggcatg ggcgaagtct
     6901 ggctggccac tcgcgacgac ggtctctacg acggccaggt cgcgatcaag accttgcacc
     6961 cgtacttcgc gggcggcgcg atgcgcgaac gttttctgcg cgaagcgcgc gtgctcgggc
     7021 ggctggcaca tcccaacatc gcccgcatgc tggacgccgg cgtccacgag ggcgtggtgt
     7081 atctcgtgct cgagtacgtg gtcggccggc agatcgaccg cgcgtgcgac gacgaaagcc
     7141 tggatgtctc cgcgcgactt gaaatcttcc tgcagctttg cgccgccgtg gcgcacgcgc
     7201 attcgagtct cgtcgtacat cgcgacatca agccggggaa cgtcctgctg acggccggca
     7261 atgttccgaa gctgctcgat ttcggcattg cgagtttctt cgagcccgag aaaggcgagg
     7321 cgcagcccgc catcacgcgg ctgaccggca ggatattcac ccccgaatac gcggcgcccg
     7381 agcaggtgct gggccaggag atcacgacgt ccaccgacgt gtactccctc ggcgtcctgc
     7441 tctatgtgct tcttgccgga cgactgccgt acgcccccgc agagggcggg cggacgcact
     7501 gggagcacgc cgtactccat gaagagccgc agcgcatggc gcgcgccctg gatcccgccg
     7561 ggcagacagc gctcgcctcg agccggtcga tgccgtacgc gcgtttgcgc cgcgaactcg
     7621 gcggcgatct cgaagtcatc gtgcagaagg cgctccggaa gcggcccgag gatcgctatc
     7681 tcacggtcgc cgcatttgcg gacgacattc gccgctatct acaaggcgag cccgtcctcg
     7741 cacgagcgga ctcgtcctgg taccggctgc gcaagttcgc gcggcgcaat cgcctggcgg
     7801 tggtttcgac cctggccgta gtgctggcgc tgggaatcgg attcgccgtg tcgttgtggc
     7861 agttgcaggt ggcacacgcg gagcgacagc gcgccgagga gtccaaggaa ttcatcgcgt
     7921 cgatctttcg ctcggccgat ccgttctata ccggcaagga gtccatgacc gcggccgaac
     7981 tactcacgct ggcgcgcgag cgcatcgaca gcgagctcgt ggcgcagccg cagaacgccg
     8041 tcgagctgct gacgctcgtc ggcgagagcc agttgaatct cagtcaagac gctgcggcga
     8101 aggcaacgct cacgaaggcc gtcgagatgg ctgaacgcct gcaaccccgc gacgacgtat
     8161 tgatcgccga ggcgcgcgcg cagatggcag gggttgcgtc ggaaaatggt gagtgggagc
     8221 aggtgcgggc catggccgcg ctggctctgc cggacttgcg caaataccag cccagaaccg
     8281 ctcgcttgct gaacgggatg ctgatctcgc tggcgtacgc cgaatccgat gacggtaatc
     8341 gcgaagccgc gattgcgctc tcccgtgaag gggtcgcggc agtgacggcg gctctggggc
     8401 ccatgcattc tgaaaccata tcgggccgct atcacctcgc cactttaatc cagcaggccg
     8461 gccggctcga cgaggcgcga gccgtggcag agcaagtctt gcgcgacgca catgccctgg
     8521 cgtcagacgg ccattccggc gcactggtga tacaggcgga ggggcgctac gggaccgtgc
     8581 tcctcgagat gggcgagctc cagtcggcga tatcgcacct caaggcggcc aacgatctgg
     8641 cggccaagct ctacggaccg aagaacccgt cgaggtacaa ctggttctcc tcgttggcca
     8701 gggcccaaca gcgactcggt gatttcaagg gtctgctggt cacgaggcgg caggcctacg
     8761 agggaaacca ggagggtggc atcagccagg caagggatct caccaatctt gccaggtcga
     8821 cgtttgcggc ccgcaaagtg caggactcgg tggacctgct gcgtcgtgcg atcgagctcg
     8881 aaaagcaata cgacgtcggc aagggttcat ggatcgtgct ggcgcagtcg gactacggcg
     8941 ctgcgctcgc gctgagtgga caactcgagc aggccgatcg cgagctcgag acggcgttgg
     9001 cgttcgccaa ggaatccgcc aaggaagaca gcctcgagtc gacctggaac gcgattggcc
     9061 tcgcacgcca ggtgcgctcg caatgggccg agagcgaaca ggcgtttcgc gaggcactcg
     9121 cgagcacgac gagcaccgcc atcaatcaga agtttcgcgc cgaagcgttg cagggcattg
     9181 gtgttgcccg attggagcga ggcgacgccg ctgacgggga aaactggctg cgccaggccg
     9241 acgagtcggt ccgcaagatg ttcgtgggca tgacgccgct gcgcgcggat atcgcgatga
     9301 acctcggcag gacgctgctg gcgcaacgga aaattgaggc ggccaatgaa tcgttcacgg
     9361 ccgcgaattc ctattggctc gactacgacg agtccaatcg aagcgcgggc atagcggcgt
     9421 actggctggc gcagggacac ctggctgcgg ccgccagctc cgaagctcga atcgagctcg
     9481 agcggtccgt cggaatcctc gagcattcgc cgttgcccgg ggacgcgaaa ctcgtgcagg
     9541 ccgcacgtcg cgacctggta cggttgccga agcagggaag ttagttcgcg ctgacgcgct
     9601 tgagccaggc gcggatatcg gtcacctcgg gctcacacag cgagtgcggc atgggatacg
     9661 catgccactc caccggataa cccgcgtctt tcagcaaccg atgcgaatcc tcgccgagcg
     9721 ggtagggcac caccgggtcc tgggtgccat gcgccatgaa gatgggcgtc gactggttgg
     9781 ccgggttgcg cgccgccgcg agttcacgcg ccagcggtag atagcaggac agcgcgataa
     9841 cgccggcaag cttttcggtg tgccgcgtgg cgatgtgcag tgccatggcg ccgccctgcg
     9901 aaaatcccgc gatcgcgatc cggttcgaag ggacgccgcg atcgttctcc gccttgatca
     9961 gggcgcccac cgctttcgcg gaggcgttga tgccgtcgaa gtcttcggcg gatcgccggt
    10021 cgatgccgat gatgtcgtac caggcgcgca tctggtaacc gttgttgatc gtcaccggcc
    10081 gcacgggcgc atgcggaaac acgaatcgca gtacgggcat ccccgcgtcg agcagttcgg
    10141 gcaccagagg ttcgaagtcg tggccgtcgg cgccgaggcc gtgcatccag atgatggaga
    10201 aggtgggatt gggtccggtt tcgaccgtga cggtttcgag tgccttcaaa caatagtctc
    10261 cgagatgttc gacgccgtga ggttcgcggc ccacatgagc tggtccagaa gttcgcgcac
    10321 gcccgggaag aacgtatacg cgagccagaa gaacaacacg gtgaatccta tccacatgaa
    10381 gccgtgcagt gcgccccagc cggggtgatg gcccgcagcc tggtgaccgc cgttgcggat
    10441 catcttgaac ggcgtcgaaa tcatgaagta gaggagggcg agcccgagaa taccgaccca
    10501 cagcggtacg ccgtgcggca gaccccagcc aaagatcgtg tgttgcgtga cgagcgatgc
    10561 catcgcgagg atccagccga cgaagatcac ggcgccgatg atggcggcga cgggtacgaa
    10621 gaccgccatg atgccgcggg cggcggggga catgttcggc gcgacgttcg ccgccgtctg
    10681 gcgcagcgtc tcgttcatgt gccggaactg ccggctcatg tgccgctgct ggcgccgcca
    10741 ctcgcggtgc cagcgatgat tggtttcatc aaagtttttt ttagcgcgat tgatgatgtc
    10801 ttcggtgttg aagggcacgc cgaaggccgc ggcccggtcc tcggcggtgt ccgcataggg
    10861 cacgatgatg agcatcgcga ggtagatgag cagcgtcacg ccggtgaaca gcgtgagcac
    10921 gaccgtgccg atgcgcaccc aggtcacatc gagacccatg tacgcggcga ggccgttgca
    10981 gacgccgccc cacatcgcgc cttcgcggat cttgaacagg cgtttgcggg gctcgggccc
    11041 gaacgaagcg ggctccgcgt tcgcggtgcc gccgtcggac gcggcgctgg ccgccttttc
    11101 gtcggcggat tccaccggac ccatctcggc gatgatgcgt tccacttccg tggtgctcac
    11161 gacggacttg tgagggccta acatcccgcg gcatttctcg ccgatggcct gctcgaggtc
    11221 ccccaggact tcgccgcgat ccgggctgtc cttgagacgc gtttcggcac ggtccaggta
    11281 ggagcgcagc aggttgaaac ccggctcctc gagttgatag gagtttccgt tgaggctgat
    11341 cgttaccact ttttgcatgg cttagttccc aaaatcggcg agagtgcggt tgagttcttt
    11401 ccagtaatca ttgagcgtcg acaattgcgt ccggcccttc tgcgtgagct tgtagtactt
    11461 gcgcggcggc ccggcttcgg attcctgcca ctcgtagtcc accagctctt cgcgccgcag
    11521 cttgctcagg aggggataca acgtcccttc ctgcgtcgcg aactcggtgg gccccaggag
    11581 cttgagcatg tcggcgacat agaccttgtc gccggccacg atcttgagga tcacgaattc
    11641 gagcaggccc ttgcggatcg gcgcgagttt ttcactgatg ttcatgggtt cagacagcta
    11701 ccttggagac gcaaggtact tgagtcaccg ctggtctgtc aacaaccgat ttcgtgccgt
    11761 acaatccgcg ccctcgttgg ggtagggcga cttggcccgc gagagacccg agtgatgttg
    11821 aaggccccga tggggctttt gtttttttgg
//
LOCUS       DATDEU010000118         4888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1043738, whole genome shotgun sequence.
ACCESSION   DATDEU010000118 DATDEU010000000
VERSION     DATDEU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043738"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..319
                     /locus_tag="VIV63_02915"
                     /pseudo
     CDS             155..319
                     /locus_tag="VIV63_02915"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
     gene            340..1719
                     /locus_tag="VIV63_02920"
     CDS             340..1719
                     /locus_tag="VIV63_02920"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEY6123577.1"
                     /translation="MLKKLVPSIVATLAGIAIGAQAMAAVSATEAAKLGAELTPLGAV
                     KAGNADGSIPAWDGGLTSAAQAGFANFRPGQHHPDPYASDKPLYTVTSANMAQYGAKL
                     TEGHKKLLTTYRATFKMPVYPTHRSAAAPQRIYDATKRIATTAQLAKGGNGVTNAGEG
                     IPFAIPKEGVEIFWNHVLRYRGDVIVRQIGQAPVTSGGSFTMVKFHDETMVAYSLPGA
                     KSENLNNTIAYFIQETVAPARLAGEVLLVQETLDQSIENRRAWVYNPGQRRVRRAPNV
                     AFDNPGTNSDNLRTSDQFDMYNGSPERYDWTLVGKKEILVPYNSYKLHSNTLKYTDIL
                     KKNHVNPELTRYELHRVWVVDSKLKAGQSHLYSRRTLYVDEDSWHILAVDCYDSRGQL
                     YRVQEGHVIQYYDVPALWTALETVYDLTNGRYLALGLDNEEPRSRDFSQKRTVADYQP
                     AALQRRGVR"
     gene            1816..2832
                     /locus_tag="VIV63_02925"
     CDS             1816..2832
                     /locus_tag="VIV63_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123578.1"
                     /translation="MRRGIGLALVATLAAGGLATIAWAQLDPNDSANRPAEIEPLAGS
                     SLLLDLAVAGQRLVAVGERGHVLLSDDQGATWRQAKAVPTRVMLTAVFFVDAEYGWAV
                     GHDETILNTADGGETWTRSHFAPEAQQPLLDLWFANRVSGIAVGAYGAYFTTNDGGRH
                     WSSAKFSAPPPTTAAPAADEELPPDYHLNRIVGVGNRLYVAAEGGKLYRSDDRGASWR
                     MLPSPYEGSFFGLVPIRGEGLLAFGLRGHLFRSADAGETWHELDSHTTAMLTDGVAVN
                     DLRVVIGGLSGVLLVSADAGETFKLTQQDDRKGMAALLPGPAGTVVVAGEGGVRTIRV
                     TEGS"
     gene            2837..>4888
                     /locus_tag="VIV63_02930"
     CDS             2837..>4888
                     /locus_tag="VIV63_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY6123579.1"
                     /translation="MTKETGSLAATLERLIFGHRLAIVIVFALLTVALSVVAARGLHI
                     DASFTKQLPLKHEYMQTFVKHQAEFGGANRVLIALVARDGNMFTPGFFDALKKATDEV
                     LVMDGIDRGRVQSLFTPNVRYMEVVEDGIEAGNVVDSEFVPTAEALAKVRENILKAGI
                     RGRLVANDFSGALVSAIVLEKDATGKPIDPIEVARTLETRVRDLIQSSDVLVQAQGSG
                     VDVHMIGFAKVVGDIADGASSVITFAIITILLTLLAVWYYVQSLKVAVVPIACSIVAV
                     TWQLGTLVLLGYGIDPLGLLVPFLIFAIGVSHGVQKISAVKEAAVAGSSSMDAARQTF
                     RQLLMPAIIALLADLVGFITILLIPVQVVQEMAITASIGVAIVILTDLILLPVLVSWV
                     KWDDKYKHRVEERQAKLTVYWAKLARITERKPAAIIIGIAVLLGMFGVWKGRETPIGD
                     TQAGVPELRADSRYNRDSNIITNKFSIGVDILTVIVETKEPACTSHELMTAIDTFAWK
                     MRNVDGVQEVMALPIVAKIAIAGWNEGSLKWRNIPREPNQLTQSTRYIETSTGLLNAD
                     CNVIPVMLFLRDHKAATIERVVTEVKAWRELNPLPGATFNLATGNVGVMAATNEEVKA
                     KEYPILGWVFAAVILMCLLTFRSLLGTVLVFVPLALVSILVYAVMAMVGIGLKVNTLP
                     MV"
BASE COUNT          881 a         1627 c         1552 g          828 t
ORIGIN      
        1 agcttggcgc tgtattgggc catgttcgcc gacgtcaccg tgtacagcgg tttgtcgctg
       61 gcgtacggat cggggtgatg ctggcccggc cggaagttgg cgaaacccgc ctgcgccgcg
      121 gatgtaaggc ccccttccca cgccgggacc gggcggcaat ttcgtcgaag gccggtacgg
      181 cctcacgttg ggcgtcgccg ccaatctgat ggcgaagtgg gagctggacg tggcgtggac
      241 caagttcggc ggcgccggcc ggttcaatga catcaatgac cgcgacttcg tcgcggccac
      301 tgcgaagttc tcgttctgat cgcgacgagg ggaaattcca tgctgaaaaa acttgttcct
      361 tccatcgttg caaccctcgc gggcatcgcg atcggcgcgc aggccatggc cgcggtatcg
      421 gccaccgaag cggcgaagct cggtgccgaa ctcacgccgc tcggcgccgt gaaggcgggc
      481 aacgccgacg gctccattcc ggcatgggac ggtggactca cctccgcggc ccaggcgggg
      541 ttcgccaact tccgccccgg ccagcatcac cccgatccgt acgccagcga caagccgctg
      601 tacacggtga cctcggcgaa catggcccag tacggcgcca agctcaccga aggtcacaag
      661 aagctgctga ccacctaccg cgccaccttc aagatgcccg tgtatcccac gcatcgcagc
      721 gccgccgctc cccagcgcat ctacgacgcc accaagcgca tcgcgaccac ggcgcaactc
      781 gccaagggtg gcaacggcgt caccaacgcg ggtgaaggca ttccgttcgc catccccaag
      841 gaaggcgtcg agatcttctg gaaccacgtg cttcgatatc gcggcgacgt catcgtccgg
      901 cagatcggcc aggccccggt cacctccggt ggctcgttca cgatggtgaa gttccacgac
      961 gaaaccatgg tggcgtacag cctgccgggt gcgaagtccg agaatctcaa caacaccatc
     1021 gcgtatttca tccaggaaac cgtggcgccc gcgcgcctcg cgggtgaagt gctgctggtg
     1081 caggagacgc tcgaccagtc gatcgagaat cgccgcgcct gggtctacaa ccccggtcag
     1141 cgccgcgtgc gccgcgcgcc caacgtcgcg ttcgacaacc ccggcaccaa ctccgacaac
     1201 ctgcgtacgt ccgaccagtt cgacatgtac aacggctcgc ccgagcgata cgactggacg
     1261 ctggtcggca agaaagaaat cctcgtgccg tacaacagct acaagctgca ctcgaacacg
     1321 ctcaagtaca ccgacatcct caagaagaat cacgtcaacc ccgagctgac tcgttacgag
     1381 ctgcatcgtg tctgggtggt cgattcgaag ctcaaggccg gccagagcca tctctacagc
     1441 cgccgtacgc tgtacgtgga cgaagacagc tggcacatcc tcgcggtcga ctgctacgac
     1501 agccgcggcc agctctatcg cgtccaggaa ggtcacgtca ttcagtacta cgacgtgccc
     1561 gcgctgtgga ccgcgctcga aaccgtgtac gacctgacga acggccgtta tctcgcgctc
     1621 ggactcgaca acgaagagcc gcgcagccgt gacttctcgc agaagcgcac ggtggccgac
     1681 taccagcccg cggccctgca gcgtcgcggc gtccggtaac gactcgctga tcaagaccac
     1741 tgacggcccg gttgattcaa atcaactggg ccgtttagtt ttagaacaat agaagaaact
     1801 tcccgggagt tgggcatgag gcgtggaatt ggcctcgcac tggtggcgac gctggcggct
     1861 ggtggcctcg cgaccatcgc ctgggctcag ctcgatccca acgattcggc caaccggccg
     1921 gcggaaatcg aaccgctggc gggaagctcg ctgttgctcg acctcgcggt cgcgggccag
     1981 cgcctcgtcg ccgtgggcga gcgcggccac gtgctgctct ccgacgacca gggcgcgacc
     2041 tggcgtcagg cgaaagccgt tccgacgcgc gtcatgctca ccgcggtgtt cttcgtcgac
     2101 gcggagtacg gctgggccgt cggccacgac gagaccatcc tcaacaccgc cgatggtggc
     2161 gagacctgga cccggtcgca cttcgcgccc gaagcgcaac agccgttgct cgacctgtgg
     2221 ttcgccaacc gcgtgagcgg cattgccgtg ggcgcttacg gcgcctactt caccaccaac
     2281 gatggcgggc gccactggtc ctcggcgaag ttctccgcgc ctccgcccac gacggcagct
     2341 ccggcggcgg acgaagaact gccgcctgac taccacctca atcgcatcgt gggcgtcggt
     2401 aaccggctgt acgtagccgc cgagggcggc aagctctatc gctccgatga tcgcggcgcg
     2461 agctggcgca tgttgccctc gccctatgaa ggcagtttct tcgggctggt gcccattcgt
     2521 ggcgagggcc tgctggcatt cgggttgcgc ggccacctgt tccgctcggc cgacgccggc
     2581 gagacctggc acgagctcga ctcccacacc acggccatgc tgaccgacgg agtcgccgta
     2641 aacgatctgc gcgtggtcat cgggggcctg agcggcgtgc tgctcgtgtc ggcggatgcc
     2701 ggcgagacgt tcaagctcac gcaacaggac gatcgcaagg gcatggcggc actgctgccg
     2761 gggcccgccg gcacggtcgt cgtggccggc gagggtgggg tacgcaccat ccgtgtcacg
     2821 gaaggtagct agggccatga cgaaggaaac cggttccctg gccgccacgc tcgagcgcct
     2881 gatcttcggc catcgcctgg ctatcgtcat cgtgttcgcg ctgctgaccg tcgcgctgtc
     2941 ggtggtggcg gcgcgcggcc tgcacatcga cgcttcgttc accaagcagc tgccgctcaa
     3001 gcacgagtac atgcagacgt tcgtgaagca ccaggcggag ttcggtggtg ccaatcgcgt
     3061 gctgatcgcg ctggtcgcgc gcgacggcaa catgttcacg cccggcttct tcgacgcgct
     3121 gaagaaagcc accgacgaag tgctggtcat ggacggcatc gaccgcggcc gcgtgcagtc
     3181 gctgttcacg cccaacgtgc gctacatgga agtcgtcgaa gacggcatcg aggccggcaa
     3241 cgtcgtcgac tcggagttcg tgccgaccgc cgaagcgctg gccaaggttc gcgagaacat
     3301 cctcaaggcg ggaatccgcg gccggctcgt cgccaacgat ttctccggcg cgctcgtgtc
     3361 ggccatcgtg ctcgagaagg acgcgacggg taagccgatc gatcccatcg aagtggcgcg
     3421 cacgctcgaa acccgcgtgc gcgatctcat ccagagcagc gacgtgctgg tgcaggcgca
     3481 gggatcgggc gtcgacgtgc acatgatcgg cttcgccaag gtggtgggcg acatcgccga
     3541 cggggcgagt tcggtgatca ccttcgcgat catcacgatc ctgctcacgc tgctggcggt
     3601 ctggtactac gtgcagtcgc tgaaggttgc cgtggtgccg atcgcctgct ccatcgtcgc
     3661 ggtcacctgg cagctcggca cgctggtgct gctgggttac ggcatcgatc ccctgggcct
     3721 tctcgtgccg ttcctcatct tcgccatcgg cgtgtcgcac ggcgtgcaga agatcagcgc
     3781 ggtcaaggag gccgcggtcg ccggcagcag cagcatggac gccgcgcgcc agacgttccg
     3841 gcaattgttg atgcccgcca tcatcgcgct gctggccgac ctcgtcggct tcatcaccat
     3901 cctgctgatc cccgtgcagg tggtgcagga aatggccatt accgccagca tcggcgtggc
     3961 catcgtcatc ctcaccgatc tcatcctgct gccggtgctc gtgtcgtggg tgaagtggga
     4021 cgacaagtac aaacaccgcg tcgaggagcg ccaggccaaa ctcaccgtgt actgggccaa
     4081 gctcgcgcgc atcacggagc gcaagcccgc ggccatcatc atcggcatcg cggtgttgtt
     4141 aggcatgttc ggtgtgtgga aagggcgcga gacgcccatc ggcgacacgc aggcgggtgt
     4201 gcccgagttg cgcgccgact cgcgctacaa ccgcgacagc aacatcatca ccaacaagtt
     4261 ctcgatcggc gtcgacattc tgaccgtgat cgtcgagacg aaagagccgg cctgcaccag
     4321 ccatgagctc atgacggcca tcgacacgtt cgcgtggaag atgcgcaacg tggacggcgt
     4381 gcaggaagtg atggcgctgc ccatcgtggc gaagatagcc atcgccggct ggaacgaagg
     4441 cagcctcaag tggcgcaaca tcccgcgcga gcccaaccag ctcacgcagt cgacgcgtta
     4501 catcgaaacc agcaccggcc tgctgaacgc cgactgcaac gtgatcccgg tgatgttgtt
     4561 cctgcgcgat cacaaggccg cgaccatcga acgcgtggtc accgaagtga aggcctggcg
     4621 ggagctcaac cccctgccgg gcgccacgtt caacctggcc acgggcaacg tcggcgtcat
     4681 ggcggccacc aacgaggaag tgaaggccaa ggaatatccc atcctgggct gggttttcgc
     4741 cgccgtgatc ctcatgtgcc tgctgacgtt ccgcagcttg ttaggcacgg tgctggtgtt
     4801 cgtgccgctc gccttggtgt ccatcctggt ctatgccgtg atggccatgg tcggaatagg
     4861 cctcaaggtg aacacgctgc ccatggtc
//
LOCUS       DATDEU010000119         2710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1481169, whole genome shotgun sequence.
ACCESSION   DATDEU010000119 DATDEU010000000
VERSION     DATDEU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481169"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /gene="hutH"
                     /locus_tag="VIV63_02935"
     CDS             <1..1338
                     /gene="hutH"
                     /locus_tag="VIV63_02935"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY6123580.1"
                     /translation="QTHIPNDQLELLQTNLVLSHAVGVGAPLPAPVVRLTLALKIATL
                     ARGYSGVRRELIDALVKLYNADVLPRIPSKGSVGASGDLAPLAHLSAVLLGVGDVECA
                     GKIMTADEGLERAGLTRIKLAAKEGLALLNGTQISTALALVNLFAIDNLYATALVAGA
                     LSVDAAEGSASPFDARIHELRGHPGQIAAAAAYRELLAGSPINSSHRDCGKVQDPYSL
                     RCQPQVMGACSDQIEHARGVLLREANAVTDNPLVFANTGLDTAEVLSGGNFHAEPVAF
                     AADNLALAVAEIGALSERRIALLIDATLSGLPPFLVRDGGVNSGFMIAHVTAAALASE
                     NKLLAHPASVDSLPTSANQEDHVSMATFAARKLGDLAENTAIILAIELLAAAQGVDLR
                     APHLTSEKLQRVMREIRSRVPHYDIDRYLAPDIAAMTDAVSGGVIAAHAPRQQ"
     gene            1335..2540
                     /locus_tag="VIV63_02940"
     CDS             1335..2540
                     /locus_tag="VIV63_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY6123581.1"
                     /translation="MSVPVEFILFGVTLLGVAVFHRHTLLVALTGLAAITAYKLFFGD
                     FYGQPGLLGLWFHLLEEWVILANLFALLVGFALLSRHFEESHVPHLMPRVLPDDWKGG
                     LALLAIVFVLSGFLDNIAAALIGATVAGTVYRRKVHIGYLAALVAASNAGGAGSVVGD
                     TTTTMMWLEGVAPLEVLHAYVAAVAAFFVFAIPAARAQHRFSPIEKNPDPQIHIDWAR
                     LIIVVLILVGAIAANIHANTLPPEQASAFPYIGATVIGVILLVTPWRRPDWKAVPAAV
                     TGSVFLLSLVLSASMMPVEELPLPSAATTFGLGFVSAVFDNIPLTKLALEQGGYDWGV
                     LAYAVGFGGSMIWFGSSAGVAVSNLYPDAKSVGQWLRHGWYVAVGYVVGFIVLMAVLG
                     WNPHTLDHR"
BASE COUNT          416 a          901 c          890 g          503 t
ORIGIN      
        1 caaacccaca ttcccaacga ccagctcgag ctgctgcaaa ccaacctggt gctgtcgcat
       61 gcggtgggcg tgggtgcgcc cctgccggcg cccgtggtgc ggctcacact ggcgttgaag
      121 atcgccacgc tggcgcgagg ctactcgggc gtgcgacgcg agctcatcga cgcgctggtc
      181 aagctctaca acgccgacgt gctgccgcgc attccctcga agggctcggt gggcgcgagc
      241 ggcgacctgg cgccactcgc acacctctcg gccgtgttgt taggtgtggg cgacgtcgag
      301 tgcgccggaa agatcatgac cgccgacgaa gggctcgagc gcgcagggct cacccgcatc
      361 aagctcgccg ccaaggaagg cctggcattg ctcaatggca cgcagatctc cacggcgctg
      421 gcgctcgtca atctcttcgc gatcgacaac ctgtacgcca ccgcgctggt ggcgggcgcg
      481 ctctcggtgg acgcggccga aggttcggcc agcccgttcg atgcgcgcat ccacgaactg
      541 cggggtcacc cggggcagat tgcggccgcg gcggcctatc gagagttgtt agccggcagt
      601 cccatcaatt ccagccatcg cgattgcggc aaggtgcagg acccgtacag cctgcgctgc
      661 cagccgcagg tcatgggcgc gtgcagcgac cagatcgagc atgcgcgtgg cgttctgctg
      721 cgcgaggcca acgcggtcac cgacaatccg ctggtctttg cgaacacggg attggacacg
      781 gccgaggtgc tgtcgggcgg caattttcac gccgagccgg tggcgttcgc ggcggacaac
      841 ctcgcgctgg ccgtggccga aatcggcgcg ttgtccgaac gccgcatcgc attgctcatc
      901 gacgcgacgc tgtcgggcct gccgccattc ctcgtgaggg atggcggcgt gaactcggga
      961 ttcatgatcg cgcacgtgac ggccgccgcg ctggcttcgg aaaacaaact gctggcccat
     1021 ccggccagcg tcgattcgct gccgacgtcc gcgaaccagg aagatcacgt gtcgatggcc
     1081 acgttcgcgg cgcgcaagct cggcgatctt gctgagaata cggccatcat cctggccatc
     1141 gaactgctgg ccgcggcgca gggcgtggac ctgcgtgcgc cgcatctgac ctccgagaaa
     1201 ttgcagcggg tgatgcgcga gatccgcagc cgcgtgccgc actacgatat cgaccgctac
     1261 ctggcgccgg acatcgcggc catgaccgat gccgtcagcg gcggtgtgat cgcggcacac
     1321 gcgccccgcc agcagtgagc gttcccgtcg agttcatcct gttcggcgtg accttgctgg
     1381 gcgtcgcggt atttcatcgc cacaccttgc tcgtcgcact caccggcctc gcggcgatca
     1441 cggcttacaa acttttcttt ggcgatttct acggacagcc cggactactc ggtctgtggt
     1501 ttcacctcct cgaagagtgg gtgatcctcg cgaacctgtt cgcgctgctc gtcggcttcg
     1561 cgctgctgtc gcggcatttc gaagagagcc acgtgccgca cctcatgccg cgcgtgctgc
     1621 ccgacgactg gaaaggcggc ctcgcgctgc tggccatcgt gttcgtcctc tccgggttcc
     1681 tggacaacat cgcggccgcg ctcatcggcg ccaccgtggc cggcacggtg taccgccgca
     1741 aggtgcacat cggttatctc gcggccctgg ttgccgcgtc caacgccggc ggcgcgggta
     1801 gcgtggtcgg cgacacgacg accaccatga tgtggctgga aggcgtggct cctctcgagg
     1861 tgttgcacgc ctacgtggcg gcggtcgcgg cgttcttcgt gtttgcgatc cccgcggcgc
     1921 gcgcgcagca ccgcttttca ccgatcgaga agaacccgga cccgcagatc cacatcgact
     1981 gggcgcgtct catcatcgtc gtgctcatcc tcgtcggggc gatcgcggcc aacatccatg
     2041 cgaacacgct tccgcccgag caggccagcg cctttcccta catcggcgcc acggtcattg
     2101 gcgtgatttt gctggtgacg ccgtggcggc gaccggactg gaaggccgtc cccgcggccg
     2161 tgacgggcag tgtgttcctg ctgtcgctgg tgttgtcggc atcgatgatg ccggtggaag
     2221 aattgccgct gccgtcggcc gcgaccacct tcggactggg gttcgtctcg gcggtgttcg
     2281 acaacatccc gctgaccaag ctggcgctgg agcagggtgg ttatgactgg ggcgtgctcg
     2341 catatgccgt gggcttcggt ggttcgatga tctggttcgg ttcgtcagcg ggtgtcgccg
     2401 tttcgaatct ctatcccgac gccaaatccg tggggcagtg gcttcggcat ggctggtatg
     2461 tcgccgtagg ctacgtcgtc ggattcatcg tcctgatggc ggtgctgggc tggaatccgc
     2521 acacgctcga tcatcgctga ggcccggcca ctcaccacaa tgaacagcgc cagcacccag
     2581 tggaaggcca cggggatggc gccttcgcgc cagaagtccg cgccgttctc attcacgttc
     2641 accgagagcc gcacggccat caccacgaag tagattgtcg cgaacatgcg cagcgcctgc
     2701 gccgcgtgtg
//
LOCUS       DATDEU010000120         6531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1626736, whole genome shotgun sequence.
ACCESSION   DATDEU010000120 DATDEU010000000
VERSION     DATDEU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626736"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1002)
                     /locus_tag="VIV63_02945"
     CDS             complement(124..1002)
                     /locus_tag="VIV63_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6123582.1"
                     /translation="MNNATVEASNLSKSFGAKQVLRDMSFGIAPGDVIGVLGKNGAGK
                     TTLLELMLGFSAPSAGAVRVFGHESYSLPGDIKARVGFVPQQDELLDQLTVADQLRVV
                     ASFYSHWDSALIDRLCSEWNVNVKARIKGMSVGERQKLSILLAFGHKPDLLILDEPVA
                     SLDPIARRQFLEQLLTLSTDEQRAIVFSSHIVSDIERLANRIWILKEGRLDWQGDLDS
                     LKESIVRLHFRGTGTELPVFKIPGVLNFRREGAFATAVVRDWTPEAQDALEKSASVQI
                     EVETLGLEEIFVELHR"
     gene            complement(995..1375)
                     /locus_tag="VIV63_02950"
     CDS             complement(995..1375)
                     /locus_tag="VIV63_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY6123583.1"
                     /translation="MFILNPQSGVPIYRQILEQVRRMVASGQLAPGTALPSVRDLAIR
                     HTINPMTISRAYSLLEAEGLLERNRGRPMTVANQSRNHSQLPKRLQQVDPLVKQTVMA
                     AKQLQLTEAELVKAIRREWEQNDE"
     gene            complement(1456..1878)
                     /locus_tag="VIV63_02955"
     CDS             complement(1456..1878)
                     /locus_tag="VIV63_02955"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY6123584.1"
                     /translation="MAENKTKPTTVSVAAFLKAKTAGQQLKDSQELVKLFKEITGKPA
                     KMWGPSIVGFDSYHYVYPSGREGDAPLIGFAPRKNELSLYMAPILEARALMAKLGKHK
                     AGAGCLYIKTLDDIDRAVLLKLAKASIAELRKRYPPSG"
     gene            complement(1871..2152)
                     /locus_tag="VIV63_02960"
     CDS             complement(1871..2152)
                     /locus_tag="VIV63_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123585.1"
                     /translation="MRVRKVCPEGHVFYKTPGCSSCPRCEAARKPEGFIAELAAPARR
                     ALEGAGLTTLARLAKKSEAQVSNLHGMGPNAIAKLRASLTKAGLSFKNG"
     gene            complement(2152..2622)
                     /locus_tag="VIV63_02965"
     CDS             complement(2152..2622)
                     /locus_tag="VIV63_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6123586.1"
                     /translation="MTKKRASITVRPVTPQDHDAWRPLWDEYNAFYERTGPTALSEEM
                     NDTLWRRFFDASEPVHCIVAEREHKVVGICHYLYHRSTSRMELLCYLQDLFTAPDYRG
                     HGIGRALIERVYEIANEAGCKRVYWQTHTTNTPGRTLYDKVAKHFGFIVYAKDV"
     gene            complement(2619..3176)
                     /locus_tag="VIV63_02970"
     CDS             complement(2619..3176)
                     /locus_tag="VIV63_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123587.1"
                     /translation="MVKLSIVLVSLLFPIAAGAQSPPSKAERDAYRAKQMKLIHRAYE
                     LQPRRRDTPLRELNISDNEVREIQVVAAKHIPKVLLNISPVVTGCPCEEGPQCTEQVY
                     IVAEKGQTSVGLQLSRVRKAWQVGVVQEWWLNFDALKANRTKLTYDDYAARMDELYRV
                     FPMCVGNLVPAENTTAAVPKVESGK"
     gene            3543..5603
                     /locus_tag="VIV63_02975"
     CDS             3543..5603
                     /locus_tag="VIV63_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY6123588.1"
                     /translation="MSAKDSKTLQTIRVEATAEATSTAAKLPLSLRETPQSVTVVTRE
                     RIEDQNLLSLQDVLDNTPGIYTYAWDTERVIFTARGFVIDNLMYDGVPAEGNFSTASI
                     DETIDTAMFDRIEIVRGATGLMTGAGSPAASVNLYRKHADSRERATSLGFTAGSWNDY
                     RVDVDVSTPLTSDGDVRARFIGVYQDNESFQNFYTKEKAVLSGIVDADLSDSTRLSAG
                     FDYQDNKPRANTWGSFPLFLADGTAANWSRSVTTAPDWAFWDRKTTSAFAEVEHSFEN
                     GWALRSTLSWRRFEEDLALFYVYGFPDPQTGEGLDPYAYRADGEITALALDVHASGVV
                     SLFGREHELVIGYNGSKVENTGNEFAHGELAPVGNFFEWDGSYPEPVFETDGYLLTDI
                     DSSQNGFYVAGRFVLADRLKAIAGARYATWDTDHFYLYDSPDVTFHDEYEKVIPYAGL
                     IFDIGKSFSAFASFTEIFKPQVTLDADYNYLDPIDGNSVEFGIKGEHFDARLNTALTL
                     FETKQDNVAEAAFDEEGNPIMLDNGVQASIGIDGTRTRGFEFEASGALGERWNATLGW
                     TRYLIEDADGLAVRTFVPRTMVRGFTTWRPAALEKLTLGAGINWQSDSYTQVGGPDGG
                     ILLREGDVTQVSLMARFQVTPKISVQLNGSNLLDEKYYVLDNYDNSHFGEPLAVSASV
                     NLRF"
     gene            5688..5954
                     /locus_tag="VIV63_02980"
     CDS             5688..5954
                     /locus_tag="VIV63_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123589.1"
                     /translation="MGIAAPHNLRRPDAGIQRPFGVRITLKAGDPFRKLLGADWNKTH
                     WYGTAAERDAALTEMARKHEYSRSGDKPALKFDKVEKLHESRGL"
     gene            complement(5965..>6531)
                     /locus_tag="VIV63_02985"
     CDS             complement(5965..>6531)
                     /locus_tag="VIV63_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123590.1"
                     /translation="DGNKFFGEFRYDTVDNVNFPRRGATFELGWQGEREVGGDSQSAD
                     LMVFDQLYAHSWGRNTALFWTSGGTRLDNDVGEVRSYYSLGGFLNMSGITPGTLIGPQ
                     FAIARGVYYRQVGRGGQGFLNVPVYLGTSVELGNVWNTRSDISVDSARLNGSLFVGLD
                     TLLGPVYFATGFDEGGGSAYYLFLGRTF"
BASE COUNT         1234 a         2070 c         1983 g         1244 t
ORIGIN      
        1 ccgagtgcgg ctgcgtcatc aggatgtaga catcgatggc gatcagggcc agaccaacga
       61 tggtgaatac ccggagcacc ggcgatcccg tgaaatagga atagaccagg cgcgcgacgg
      121 ctttcatcgg tgcagctcca cgaagatttc ctcgagcccc agggtctcca cttcgatctg
      181 caccgacgcc gacttctcga gcgcgtcctg tgcttccggc gtccagtcgc gcaccaccgc
      241 cgtggcgaat gcgccttcgc gccggaagtt gagaacgccg ggaatcttga acacggggag
      301 ttccgtgccc gtgccgcgga agtgcaggcg cacgatcgat tccttgagcg aatcgagatc
      361 gccctgccag tcgagccgcc cttccttgag gatccagatg cgattcgcga gccgctcgat
      421 gtctgaaacg atgtgcgacg aaaacacgat ggcgcgctgc tcatcggtcg agagcgtcag
      481 cagttgttcg aggaactgcc gccgtgcgat ggggtcgagg cttgccaccg gctcgtcgag
      541 tatcagcaga tcgggcttgt gcccgaacgc gagcagtatc gacagcttct gccgctcgcc
      601 caccgacatg cccttgatgc gtgccttcac gttcacgttc cactcggaac acaggcggtc
      661 gatgagcgcg gaatcccagt gcgaatagaa cgaggccacg acgcgcagct ggtcagccac
      721 ggtgagctgg tccagcaatt catcctgctg cggcacgaag ccgacgcgag ccttgatgtc
      781 gcctggcagg ctgtagctct cgtgcccgaa tacgcgcaca gcgcccgccg acggcgcgga
      841 gaatcccagc atcaactcga gcagcgtggt tttaccggcg ccattcttgc cgagcacgcc
      901 gatgacgtcg ccaggcgcga tgccgaaaga catgtcgcga agcacctgtt tcgctccgaa
      961 cgatttgctc aggttcgagg cttcgacagt cgcgttattc atcgttctgc tcccattcac
     1021 ggcggatcgc tttcacgagc tccgcttcgg tcagttgaag ttgtttggcg gccatgacgg
     1081 tttgtttgac cagcggatcg acctgctgca gccgcttcgg cagctgcgaa tggttacggg
     1141 actggttggc gacggtcatg ggccggccgc gattgcgctc gagcagccct tccgcttcca
     1201 gcaggctgta ggcgcgggag atcgtcatcg gattgatggt gtgccggatc gcgagatcgc
     1261 gaaccgacgg cagtgccgtg ccgggcgcga gctgaccgct cgccaccatg cgccttacct
     1321 gctcgaggat ctggcggtag atcggaacgc cggattgcgg gttgagtatg aacacggaga
     1381 gcctggtgac cttgtgtatc gagacattaa tacacttacc aggacactgt cgagcgccgc
     1441 gtcacactct cattgtcagc cgctgggcgg gtatctcttc cgcaattcgg cgatcgatgc
     1501 cttcgccagc ttcagcagca ccgcgcggtc gatgtcatcg agcgtcttga tgtaaagaca
     1561 gcccgcaccg gccttgtgtt tgccgagttt cgccatgaga gccctcgctt cgagaattgg
     1621 cgccatgtat agcgacaact cgttcttgcg cggcgcaaaa ccgatgagcg gcgcgtcgcc
     1681 ttcgcggcca ctcggataga catagtgata ggagtcgaat ccgacgatgc tcgggcccca
     1741 catcttcgcg ggtttgccgg tgatctcctt gaacagcttc accagttcct ggctgtcttt
     1801 caattgctgg ccggcggtct tcgctttcag gaatgccgcg acactgaccg tggtgggttt
     1861 ggtcttgttc tcagccattc ttgaaactca agcctgcttt agtgagtgac gcacgcaatt
     1921 tggcgatggc attcgggccc atgccatgca ggttgctcac ctgcgcttcg cttttcttcg
     1981 cgagccgggc cagcgtcgtg agccccgcgc cttccagcgc acgccgcgcg ggtgctgcga
     2041 gttcagcgat aaaaccttcg ggcttgcgtg ccgcctcgca ccgcggacaa ctcgagcatc
     2101 ccggcgtctt atagaagaca tgcccctcgg ggcagacctt gcgtacgcgc atcaaacatc
     2161 cttggcgtag acgatgaacc cgaagtgctt tgccaccttg tcgtacaacg tgcgtcccgg
     2221 tgtgttcgtc gtgtgcgtct gccagtacac gcgcttgcag cccgcctcgt tcgcgatttc
     2281 gtagacgcgc tcgataagcg cgcggccgat gccgtggccg cggtagtcag gcgccgtgaa
     2341 cagatcctgc aggtaacaca acaactccat gcgcgaagtg ctgcggtgat agaggtagtg
     2401 gcagatgccg acgaccttgt gctcgcgttc ggccacgatg caatgcaccg gctcgctggc
     2461 gtcgaagaag cggcgccaga gcgtgtcgtt catctcctcg gacagcgccg tgggccccgt
     2521 cctttcgtag aacgcgttgt attcatccca gagaggtctc catgcgtcgt ggtcctgagg
     2581 tgtgactggc cggacggtga tggacgcacg ttttttcgtc atttaccgga ttctactttc
     2641 ggcacggctg ccgtggtgtt ttccgcgggc acgaggttgc cgacacacat cggaaagaca
     2701 cgatatagct cgtccattcg ggccgcatag tcgtcgtagg tcagcttcgt gcgattcgcc
     2761 ttcagcgcgt cgaagttgag ccaccattcc tgaacgacgc ccacctgcca cgctttacgg
     2821 acgcgcgaga gctgcagccc gacggatgtc tgccccttct cggccacgat gtacacctgc
     2881 tccgtgcact gcggaccctc ttcgcacgga cagccagtga cgaccgggga tatgttcagg
     2941 aggactttcg gaatgtgttt cgcggccacg acctggatct cgcggacttc gttatccgag
     3001 atgttgagtt cgcgaagcgg tgtgtcccgg cgccgcggtt gcaattcgta ggcacgatgt
     3061 atcagtttca tctgctttgc ccgatacgcg tcgcgctcgg ccttcgacgg tggcgactga
     3121 gcgccggccg caatcgggaa taacagcgat accagcacaa tcgaaagctt gaccatgttt
     3181 gcgatctccc cgggtctgga tgctattggg cacggccgcg gccgcgcaaa tgcccgaacg
     3241 gattgagggt ttttttcggg caaaggttga agggaagcca tgacaaaacg gtaaagcctg
     3301 ccgcggggct gtctttctca aataagaata attatcatct ggccctcaaa ccggcccttc
     3361 agggccttcc gggaccagcc agtcactgct cgaccctcaa gggtccgagt ccgccagcca
     3421 tccccttctt atctatgtgg cttcaagcca ccgaaggatt ggtcatgcgg ttcagaactg
     3481 ttgtgttgtc tttggcgtgc ctcaccggcg cgctcgcctc cccggctttc gcggcagaac
     3541 ctgtgagcgc gaaagactcg aagacgttgc agacgatccg cgtcgaagca acggccgaag
     3601 ccacgagcac cgcggccaaa ctaccgctct cgttgcgcga aacgccgcaa tcggtaacgg
     3661 tcgtcacgcg cgaacgcatc gaggaccaga acctgctgtc gctgcaggac gtgctcgaca
     3721 acacacccgg catctacacc tacgcgtggg ataccgagcg cgtgatattc accgcgcgcg
     3781 gattcgtcat cgacaacctg atgtacgacg gcgtgccggc cgagggcaat ttcagcaccg
     3841 cgtcgatcga cgaaacgatc gacaccgcga tgttcgaccg catcgagatc gtgcgcggcg
     3901 cgacgggcct catgaccggc gcgggcagcc ccgccgcttc ggtcaacctg tatcgcaagc
     3961 acgcggacag cagggaacgg gcgacgagcc tgggattcac cgcgggttca tggaacgact
     4021 accgggtcga cgtggacgtc tcgacgccgc tcacgagcga cggcgacgtg cgcgcgcgct
     4081 tcatcggcgt gtaccaggac aacgagtcgt ttcagaattt ctatacgaaa gagaaggcgg
     4141 tgctttccgg catcgtcgac gccgacctgt cggacagcac caggctctcc gcaggattcg
     4201 attatcagga caacaagccg cgagcaaaca cctggggctc gttcccgctg ttcctcgccg
     4261 acgggactgc ggccaactgg tcacgctcgg tgacgacggc gcccgactgg gcgttctggg
     4321 atcgcaagac cacctccgcg ttcgccgagg tcgagcattc cttcgagaac ggctgggcat
     4381 tacgcagcac actgagctgg cgccggttcg aggaagacct cgccttgttc tacgtgtacg
     4441 gattcccgga tccccagacc ggcgaaggtc tcgaccccta cgcctatcgc gccgacggcg
     4501 agatcaccgc gctcgcgctc gatgtgcacg catccggcgt cgtgagcctg ttcggccgcg
     4561 aacatgagct ggtcatcggc tacaacggct cgaaggtgga aaacacgggc aacgagttcg
     4621 cgcacggcga actcgcgcct gtcggcaact tcttcgaatg ggatggctca tatcccgaac
     4681 ccgtgttcga aacggacggc tacctgctca ccgacatcga ctcgagccag aacggctttt
     4741 atgtcgccgg ccgcttcgtg ctcgccgacc gcctcaaggc gattgccggc gcgcgttacg
     4801 ccacctggga caccgaccac ttctatctat atgactcgcc cgacgtgacc ttccacgacg
     4861 aatacgaaaa ggtcattccc tacgccggcc tgatcttcga catcggcaag agtttttcgg
     4921 cctttgcgag cttcaccgag atcttcaagc cgcaggtcac gctggatgcg gactacaact
     4981 acctcgaccc gatcgacggc aacagcgtgg agttcggcat caagggtgaa cacttcgacg
     5041 cgcggctcaa cacggcgctt acgttgttcg aaacgaaaca ggacaacgtc gccgaggccg
     5101 cattcgacga agagggcaac cccatcatgc tcgacaacgg ggtgcaggcc tcgatcggca
     5161 tcgatggcac gcgtacacgt ggcttcgagt tcgaagcgtc gggcgctctc ggcgaacgct
     5221 ggaacgcgac gctcggctgg acgcgctacc tcatcgaaga tgcggacggc ctcgccgtgc
     5281 gcaccttcgt gccgcgcacg atggtccgcg gcttcacgac gtggcggccc gctgcgctcg
     5341 agaagctgac gctgggcgcc ggcatcaact ggcagtccga cagctacacg caggtggggg
     5401 gccccgatgg gggaatcctg ctgcgcgaag gcgacgtcac gcaggtcagc ctcatggcgc
     5461 gcttccaggt tacgccgaag atttcggtgc agctgaacgg cagcaacctg ctcgacgaga
     5521 agtactacgt gctcgacaac tacgacaatt cgcacttcgg cgagccgctg gcggtgtcgg
     5581 catccgtgaa cttgcgcttc tgagcgcccg ttgcgggaga aacttgcggc cggcagcgac
     5641 gcttcgtacc ctagcgccgc tgtcggctcc tgccgcacgg agaattcatg ggtattgccg
     5701 ctcctcacaa cctgcgtcgg cccgacgcgg gcattcagcg tcccttcggc gtgcgcatca
     5761 cgctgaaagc gggcgatccg ttccgcaagc tgctcggcgc ggactggaac aagacgcact
     5821 ggtatggcac cgccgccgaa cgcgatgcgg cgctgaccga gatggcgcgc aagcacgaat
     5881 attcacgctc cggcgacaag cccgcgctga agttcgacaa ggtcgagaag ctgcacgaga
     5941 gccgcgggct ctaagcgtaa gctgttagaa agttctacca aggaacaggt agtacgccga
     6001 gccaccgccc tcgtcgaagc ccgtggcgaa atacaccggg cctaacaacg tgtccaggcc
     6061 gacgaacagg ctgccgttca gccgcgcgga atccaccgag atgtcgctcc gggtattcca
     6121 gacgttgccc aattcgaccg atgtgccgag gtacacggga acgttgagga acccctgccc
     6181 gccccggccc acctgccggt agtagacgcc gcgcgcgatg gcaaattgcg ggcctatcaa
     6241 cgttccgggc gtgatacccg acatgttgag gaatccgccc aacgaataat acgagcgcac
     6301 ctcaccgaca tcgttgtcga gccgcgtgcc gccgctggtc cagaacagcg cggtattgcg
     6361 cccccaggaa tgcgcgtaga gctggtcgaa caccatcagg tccgcgctct gcgagtcgcc
     6421 gccgacttcg cgctcgccct gccagcccag ctcgaaggtc gcgccgcgcc gcgggaagtt
     6481 gacgttgtcg accgtgtcgt agcgaaactc gccgaagaac ttgttgccgt c
//
LOCUS       DATDEU010000121         6177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1918571, whole genome shotgun sequence.
ACCESSION   DATDEU010000121 DATDEU010000000
VERSION     DATDEU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918571"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="VIV63_02990"
     CDS             <1..1417
                     /locus_tag="VIV63_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123591.1"
                     /translation="YYVDGDTSEVTKRYLRESVTQMLEEYPDLDGIGVSHGEGMGGMT
                     PLARQQFVDEVYIAGALNAKRKQPVKLIHRVPFSSGLSSGPGVSADVEKITRAAMEKL
                     GKKFSGPIWAEMKFNWSHGHSTPKLVKVHGGKLGDTYFKPAPSNYKVVWQVRNEDFFA
                     LRWGVPDFIREHIALNGKQEYVGGYFVGSETYIPALDYFTASQKPVDWKWAFQRQWLF
                     YKLWGRLLYDPKTPDTLFQAEFTRRYGKRGENLLRAYALASNTQMRLASLYDSRWDFT
                     LYGEGMLALQGEETHYIGVDALINQPTLDPAYVSVADFVKATHAGTTFGAGRITPLQL
                     ADLLERDCAEAMRLVHGIEPDDDASLMYEVEDVRAWTQLGLHLANKLRGAVALATYRA
                     YGGEEVKMQAIRYLEMAQANWALLSARTRPIYRDMRLTHYNGNAFEANPDNLFHWERV
                     RAEVARDVEVAIAAKAPAAGN"
     gene            1443..1835
                     /locus_tag="VIV63_02995"
     CDS             1443..1835
                     /locus_tag="VIV63_02995"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6123592.1"
                     /translation="MKVIINIDVPELAPAIAFYTAALGLTHSRTLDDDVAELTGASAT
                     LYLLQKDPGTAAVRKPPIDRDYHRHWTPVHFDLVVPDVDAAAARALAAGAKQETGHID
                     WRGSRCISFSDPFGHGFCFIQFEHGQYD"
     gene            complement(1905..3278)
                     /locus_tag="VIV63_03000"
     CDS             complement(1905..3278)
                     /locus_tag="VIV63_03000"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY6123593.1"
                     /translation="MKHINRRLTTVAIAGAYVAMASAQTVAPSADFHRFKPSTLRVDP
                     EAAVTEGPAASTLSVPGEGGSGGTYREAPAAFDNRTNGFDPQGPAFDTIDEETVVPLR
                     SFNDNRFIFEEVEGVADGLGPTFNAQACRECHQNIVTGGASQIAEHRTGRLELLQFVE
                     SVGGSLIQSRSTHPDIFERVPFEDSVSTLRISTNTLGAGFIEAVSNSTLLNIRDRQPA
                     AIRGTALEVAVLEANNAPRLGRFGWKNQHASLESFAADAYLNEMGITTPVLPDENTSL
                     GRFVGFGTPYDPVADPEDDGIDVVAFANFMRATKAPPRGPINSTVRAGETLFTSVGCS
                     GCHVATLRTAAPGTRINGGALRVRDAVGNKIIHPYSDFLMHDIGTGDGIPLLPGAEFV
                     STASQIRTAPLWALRTRNRLMHDGLSFTKQEAIARHAGQAAGVKTAYDALTTAQKDQV
                     LAFLDSL"
     gene            complement(3415..4206)
                     /locus_tag="VIV63_03005"
     CDS             complement(3415..4206)
                     /locus_tag="VIV63_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY6123594.1"
                     /translation="MAKPPRTIPKAVRELCLAFPCAEEFESHGTPNFRAREGSRKGKV
                     FAMFTLNHHGDGHVALWLNTPAIEQSRLIASAPKHLYKPQYVGPSGWIGVELNRGFSW
                     KRVVELVHMAYANSSPARLVAQVPKPPTVAAPTVAMTPAEIDRLKTPKALKLRDRICK
                     ICLALPETDESTQFGSPVWRVGKKSFAVLYDYGKGLTVQFWVGIERQGPLEMDPRFSI
                     PAYMGHNGWIALDVSNSVNERELRDYLLESYRHFATRRALAKLET"
     gene            complement(4199..4756)
                     /locus_tag="VIV63_03010"
     CDS             complement(4199..4756)
                     /locus_tag="VIV63_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123595.1"
                     /translation="MRVWAYLLAGISVASVAVAEEDIASRMINKETNGAWHFQPEKPK
                     AKHIKAEVPGEYAFRVKATKGANPWDVQASSPIAGAINEGDVIMLHYFARAEVPADGG
                     SALTARIQLAAAPYTSVLDMTSKISGEWTSYCAFRVASASIGENKSNVSIHLATAAQV
                     IDLGPVLVFNFGKGYDTKKLAFCDG"
     gene            complement(4778..5671)
                     /locus_tag="VIV63_03015"
     CDS             complement(4778..5671)
                     /locus_tag="VIV63_03015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6123596.1"
                     /translation="MERKTAHDFDQELLILFDAYVHGALDRRGFLDKAAKYAVGGVTA
                     AMLLDQLSPKFVAAQVIKPEDPRIKTQYVEYDSPKGYGKMRGYLAQPAKAAGKLPGIL
                     VIHENRGLNEHIEDVARRAALGGYIAIAPDGLSVAGGAPADQEAARDLFAKTDAARIA
                     GDVLAGVAWLAGNPLCNGKIGAVGFCYGGGLALRAAIETEGVDAAVCFYGRQLSVEET
                     QRLKVPVLLNYAGSDERINAGIPAFRTALDTVGVPYSLNMYPGTQHGFHNDSSAARYN
                     EAAAKLAWSRTMDFFDTYLKT"
BASE COUNT         1109 a         1973 c         1916 g         1179 t
ORIGIN      
        1 ctactacgtc gacggtgaca cctccgaggt cacgaagcgc tacctgcgcg agagcgtgac
       61 ccagatgctc gaggagtatc cggatctcga cggcatcggc gtgtcgcacg gcgaaggcat
      121 gggtggcatg acgccgctcg cgcgccagca attcgtggac gaggtgtaca tcgccggcgc
      181 gctgaacgcg aaacgcaagc agcccgtgaa gctcatccac cgagtgccgt tctcctcggg
      241 cctttcttcc ggtcccggcg tcagcgccga tgtcgagaag atcacgcgcg cggccatgga
      301 gaaactcggc aagaagttct ccggccccat ctgggcggag atgaaattca actggtcgca
      361 cggccattcc acgccgaagc tcgtgaaggt gcacggcgga aagctgggcg acacctattt
      421 caaaccggcg ccctcgaact acaaggtcgt ctggcaagtg cgcaacgaag attttttcgc
      481 gctgcgctgg ggcgtgccgg atttcattcg cgagcacatc gcgctcaacg gaaagcagga
      541 atatgtaggc ggctacttcg tcggctcgga aacgtatatt ccggcgctcg actacttcac
      601 tgcctctcag aaacccgtgg actggaaatg ggctttccag cgccagtggc tgttctacaa
      661 actctggggc cgcctgctct acgacccgaa aacaccggat acgctgttcc aggcagaatt
      721 cacgcgccgc tacggcaaac gcggcgagaa cctgctccgg gcttatgcgc tggcatccaa
      781 tacgcagatg cgcctggctt ccttgtatga ctcgcgctgg gacttcacgc tgtatggcga
      841 gggcatgctc gcgctgcagg gcgaggagac gcactacatc ggcgtggacg ctctcatcaa
      901 tcagccgact ctggatccgg cgtatgtgtc ggtggccgac ttcgtgaaag ccacgcacgc
      961 aggcacgact ttcggcgccg gccgcatcac gccgctgcag ctcgccgacc tgctcgagcg
     1021 agattgcgcg gaggcgatgc gtctcgtgca tgggatcgag cctgatgacg acgcctcact
     1081 gatgtacgaa gtggaggacg tccgggcctg gacgcaactc ggcctgcatc tggcgaacaa
     1141 acttcgcggc gccgtggcat tggcaaccta tcgcgcctat ggcggcgaag aggtcaagat
     1201 gcaggccatc aggtacctcg agatggctca ggcgaattgg gccctgttga gtgccaggac
     1261 ccgacccatc taccgcgaca tgagactcac gcactacaac ggcaacgcgt tcgaagcgaa
     1321 ccccgacaac ctgtttcact gggaacgcgt ccgcgccgaa gtggcgcgcg atgtggaagt
     1381 cgcgatcgcc gcgaaggcgc ccgctgccgg gaattgagaa cgaggacccc aggagctttc
     1441 tcttgaaagt catcatcaat atcgacgtgc ccgagcttgc gcccgccatc gccttttaca
     1501 ccgcggctct cgggctcacc cattcgcgta cgctcgacga cgacgtcgcc gagctcaccg
     1561 gcgcttcggc gactctctat ctattgcaga aggacccggg cactgccgcg gtccgcaaac
     1621 cgccgatcga tcgcgactac catcggcact ggacccccgt gcatttcgac ctggtggtcc
     1681 ccgacgtcga cgccgcggcg gcgcgagcgc tggccgctgg cgcgaagcag gaaaccggcc
     1741 acatcgactg gcgcggctcg cgctgcatca gcttcagcga tccattcggc cacggtttct
     1801 gtttcatcca gttcgaacac ggtcaatacg actaagtcgg cgggccgcgg gtcagtccat
     1861 tgacccgcga acccgcctac tctctcgagc tgcttccctt cagctcacag tgagtcgagg
     1921 aatgccagta cctggtcttt ctgcgccgtc gtcagcgcgt cgtacgccgt cttcacgccc
     1981 gcggcctgcc ccgcatgacg cgcgatcgcc tcctgtttgg tgaatgacag gccatcgtgc
     2041 atgagccggt tgcgcgtgcg cagcgcccac agaggcgccg tgcggatctg gctcgcggta
     2101 gacacgaact ccgcgcccgg cagcaaggga atgccatcgc ccgtgccgat gtcgtgcatc
     2161 aggaaatcgc tgtacggatg aatgatcttg ttccccaccg catcgcgcac gcgcagcgca
     2221 cctccattga tacgcgtgcc gggagcggcc gttcgcaacg tcgccacatg gcagccgctg
     2281 caaccgaccg acgtgaacaa cgtctcgccc gcacgcacgg tcgagttgat cggcccgcgc
     2341 ggcggcgcct tggtggcgcg catgaagttg gcgaacgcca ccacgtcgat gccgtcatcc
     2401 tccggatccg cgaccggatc gtagggagtg ccaaacccca cgaaacgccc caacgacgtg
     2461 ttttcgtccg gcaacaccgg cgtggtgatt cccatctcgt tcagataggc gtcggcggca
     2521 aacgattcga gactcgcatg ctggttcttc cacccaaaac gcccgagacg cggcgcgttg
     2581 ttggcttcga gcaccgccac ttccagggcc gtcccccgga tcgccgcggg ttgccggtcg
     2641 cgaatgttca gcaacgtcga gttggacacg gcctcgatga agccggcacc gagcgtgttg
     2701 gtggaaatcc gcagcgtgct cacgctgtct tcgaaaggca cacgttcgaa gatgtccgga
     2761 tgggtcgaac gcgactggat cagcgagccg cccaccgact ccacgaactg cagcagctcg
     2821 agacgaccgg tgcggtgctc ggcgatctgg ctcgccccgc cggtcacgat gttctggtga
     2881 cattcgcgac aggcctgtgc gttgaacgtg ggaccgaggc cgtctgcaac tccttccacc
     2941 tcctcgaaga tgaatcgatt gtcattgaag gaccgaagcg gcacgacggt ctcctcgtcg
     3001 atcgtgtcga atgccgggcc ctgcgggtcg aagccattgg ttcgattgtc gaaagcggcc
     3061 ggcgcctcac ggtaagtgcc acccgagcca ccctctcccg gcacggacaa agtgcttgcg
     3121 gccgggccct ccgtcacggc cgcttcggga tcgacgcgca gcgtcgacgg tttgaaacga
     3181 tgaaagtccg cactgggcgc caccgtctgc gcagacgcca tggccacgta cgctcctgcg
     3241 atcgcgactg tagttagtct ccgattgatg tgtttcatga attctccctc catggagaaa
     3301 ccttgactgc ggaccgaaga tagtccgtag gaggaatctt gcaaatgact ccattgggtg
     3361 acacttttcg tggcaccgcg ggaattcact tgcgttggcg aaaggcaacg aacgctaggt
     3421 ttcgagcttc gcgagggcac gccgggtggc gaagtgccgg tagctttcca gaaggtaatc
     3481 gcgcagttcg cgttcattca cgctgttcga aacatcgagc gcaatccagc cgttgtgccc
     3541 catgtacgcc ggaatgctga atcgcgggtc catttcgagc ggaccctgtc gctcgatgcc
     3601 gacccagaac tgcacggtga gtccctttcc gtagtcgtag agaacggcaa acgatttttt
     3661 gcccacgcgc cacaccggag aaccgaactg cgtgctttcg tcggtttcgg gcaacgccag
     3721 gcagatcttg caaatgcgat cgcgcaactt gagcgccttc ggtgttttca accggtcgat
     3781 ttctgccggt gtcatcgcga ccgtcggcgc ggcgaccgtg ggaggtttcg gcacctgcgc
     3841 gacgagacgc gcgggcgaac tgttcgcgta ggccatgtgc acgagttcga ccacgcgctt
     3901 ccaggaaaat ccgcgattga gctcgacgcc gatccagccg gatggcccga cgtactgcgg
     3961 tttgtagagg tgtttcggcg ccgaggcgat gagccgcgac tgctcgatcg ccggtgtgtt
     4021 gagccacagg gctacgtggc catcaccatg gtgattgagc gtgaacatcg cgaatacctt
     4081 gcctttgcgg ctcccctctc gcgcgcggaa gttaggcgtc ccgtgcgact cgaattcctc
     4141 ggcgcacggg aacgccaggc acaactcgcg caccgctttc ggaatggtgc gcggcggctt
     4201 agccatcgca gaacgcgagc ttcttcgtgt cgtagccctt gccgaaattg aacacgagca
     4261 cgggccccag atcgatgacc tgcgcggccg tcgccaggtg gatggacacg ttgctcttgt
     4321 tttcgccgat gctggcgctg gcaacgcgga aggcgcagta actcgtccac tccccggaga
     4381 tcttcgacgt catgtcgagc accgacgtgt atggagcggc cgcgagctgg atgcgcgcgg
     4441 tgagcgcgct gccaccgtcc gccggcacct cggcgcgcgc gaaatagtga agcatgatca
     4501 cgtcaccttc gttgatggcg ccggcgatcg gtgaactcgc ctgcacgtcc cacgggttcg
     4561 cgcccttggt cgctttcacg cgaaaggcgt actcacccgg cacctcggcc ttgatgtgtt
     4621 tcgctttcgg cttttccggc tggaaatgcc aggcgccgtt ggtttccttg ttgatcatgc
     4681 ggctggcgat gtcttcctcg gcgacggcca ccgacgccac ggaaatacct gccagcagat
     4741 aggcccacac cctcatgcga tccccctgtt ttatcgatta tgttttgagg tacgtatcga
     4801 agaaatccat cgtccggctc cacgcgagct tcgcggccgc ttcgttgtag cgcgccgcgc
     4861 tcgagtcgtt atggaatccg tgctgcgtgc ccggatacat gttgagactg tagggcacgc
     4921 ccaccgtgtc gagcgcggtg cgaaaggcgg gaatgcccgc gttgattcgc tcatcggacc
     4981 cggcgtagtt gagcagcaca ggcactttca atcgctgcgt ttcttcgacg ctgagttgcc
     5041 ggccgtagaa gcagaccgcc gcatccacgc cttccgtttc aatggctgcg cgcagcgcga
     5101 gtccgccgcc gtagcagaat cccacggcgc cgatcttgcc gttgcagagc gggttgccgg
     5161 caagccatgc gacgcccgcg agcacgtcgc cggcgatacg cgccgcgtct gtcttggcga
     5221 agaggtcgcg cgccgcttcc tgatcggccg gcgcgcctcc ggcaacggag aggccgtcgg
     5281 gcgcaatggc gatgtagcca cccagcgccg cgcggcgcgc cacatcttcg atgtgctcgt
     5341 tcagtccgcg gttttcgtgg atgaccagga tgccgggcag cttgcccgcc gccttggccg
     5401 gctgcgccag gtagccgcgc atcttgccgt agcccttcgg cgagtcgtac tcgacgtatt
     5461 gcgtcttgat ccgcgggtcc tcaggcttga tcacctgcgc cgccacgaac ttcgggctga
     5521 gctgatcgag cagcatcgcg gcggtcacgc cgccgaccgc gtacttggcg gccttgtcga
     5581 ggaagccgcg gcggtcgagc gcaccatgca cgtaggcgtc gaacaggatg agcagctcct
     5641 ggtcgaaatc atgagcggtc ttgcgttcca ttttcagtcc tccagaaaat ccggcgagtc
     5701 cggcagggtg gatgccctcg tctgctggac ggtggcagat gcattactga aataccgggc
     5761 tgggcgctta catgcatttc ttacgcagcc ttttctgcgg ctactgagga gcttggccgg
     5821 caggtgattc ggcccccgtt ttgctgcgat gctccgctgc tggagtcctc gtccagcacg
     5881 tcctgcaagc gttccgtcgg cctgcggatc cccgccggcg gctgcagttc gggttgaggt
     5941 atggattctg agcggcggct cggcggtcgg gggtcggcgg agagcttcac aggaaccgcg
     6001 aagggcgatt gcggcgacat gccacttgtc tcaaaggcaa caggagaagg agagaaggag
     6061 aggagccaaa ctccgtcgcg ctcaggcgtt ggcgcgccaa ctcagagccg tcaatacaga
     6121 gaaggtattc tccccttctc ctcttctcct gttgcatttg agacgagcgg gtcaaga
//
LOCUS       DATDEU010000122         5301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1044164, whole genome shotgun sequence.
ACCESSION   DATDEU010000122 DATDEU010000000
VERSION     DATDEU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044164"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VIV63_03020"
     CDS             <1..574
                     /locus_tag="VIV63_03020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6123597.1"
                     /translation="GPGLADRHFYPLYEKMCELGVPAMIHVSEACNTPYFQTTGSYYL
                     AADTVAFMQMMTSDLFKDFPDLKLIIPHGGGAVPFHWGRFRGLADMLKRPPLAESVLG
                     NVYFDTCVYHQPGIDLLVKVIDPANILFGSEMLGAVRGIDLETGFHFDDTRRYIDALT
                     VPTDTKRRIFELNTRRVYPRLDAQLKARGL"
     gene            571..1455
                     /locus_tag="VIV63_03025"
     CDS             571..1455
                     /locus_tag="VIV63_03025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6123598.1"
                     /translation="MSQFQSDPGWLSYHASPRKPGFVPPPGAVDAHCHVFGPGDVFPY
                     APERKYTPTDAPKEKLWALRDFLGFERNVIVQGTCHGADNRALTDALRSSGGRARGVA
                     SVKRDITDSQLADLHDAGVRGVRFNFVKRLVDVTPPAVLLEVAQRIAPLGWHVVVYFE
                     AQDLPELAPFIASIPTIVVVDHMGRPDVTLRPDGPEFERFVGFMREHANVWSKVSGAE
                     RLSAIGPPDYSDFVPFARRIVETFPERVLWGTDWPHPNMKSHMPDDGDLVNLLPRIAP
                     TAELQRSLLVDNPMRLYW"
     gene            1452..1820
                     /gene="ligA"
                     /locus_tag="VIV63_03030"
     CDS             1452..1820
                     /gene="ligA"
                     /locus_tag="VIV63_03030"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HEY6123599.1"
                     /translation="MKSYADIPGTTVFDAEQSRKGYHLNMFCMSLMKAPNRERFKANE
                     RAYLDEWPMTEDQKLAVIARDYNRMISLGGNIYFLAKIFATDGKSFQHAAALMTGMSQ
                     EDYARMMLNGGRAYVPKEGV"
     gene            1820..2659
                     /locus_tag="VIV63_03035"
     CDS             1820..2659
                     /locus_tag="VIV63_03035"
                     /inference="COORDINATES: protein motif:HMM:NF009901.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HEY6123600.1"
                     /translation="MARITAGVSCSHVPAIGAAIDLGKGQEPYWAPLFAGFEFSKKWM
                     AANTPDVVFLVYNDHATAFSLDFIPTFAIGCAAEFTPADEGWGARPVPVVKGAPELAA
                     HIAASVIESDFDLTLVNKMDVDHGLTVPLSLMFGQPKAWPCLVIPFAVNVVQYPPPSG
                     NRCYQLGKAIRRAVDSFGQDLNVQVWGTGGMSHQLQGPRAGLINKDFDHKFLDGMVRD
                     PEALTRIPREQYLREAGSEGIELVMWLIMRGALEGPVREVHRFYHVPASNTAVGHLIL
                     EPA"
     gene            2656..3612
                     /locus_tag="VIV63_03040"
     CDS             2656..3612
                     /locus_tag="VIV63_03040"
                     /inference="COORDINATES: protein motif:HMM:NF040065.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6123601.1"
                     /translation="MTQPIKIVVAGAGAFGQKHLDAVKLIDGIEVASIVGRELAPTEE
                     IARKYGVAHATTDLAEALARPGVEAAILCTPTQLHAAQTIQCLNAGKHVQVEIPLADS
                     LADAETVARLAKSSGLVCMVGHTRRFNPSHQWVHRRITAGEIAIQQMDVQTYFFRRTN
                     MNALGQPRSWTDHLLWHHAAHTVDLFRYQTGSEIVRANALQGPVHPTLGIAMDMSIQL
                     LAANGALCTLSLSFNNDGPLGTFFRYICDRGTYIARYDELIDGKNNAIDVSKVDVSLN
                     GIELQDREFFAAIREKREPNSSVAQVLPCYRTLNDLEKQLCR"
     gene            3603..4718
                     /locus_tag="VIV63_03045"
     CDS             3603..4718
                     /locus_tag="VIV63_03045"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY6123602.1"
                     /translation="MPLTQPPFRADHVGSLLRPPSLTRARAEHKAGRIDDVALKAAED
                     EAILGAVRMQEKLGLQGITDGEMRRSSWHMDFLYQIGGVRKIQQNITVHFHSDKGDVD
                     FTPSGLRVEGKLGLDGCIFGDHFDYLKSVVTRGTPKLTIPSPSMMHYRGGRAAIDETI
                     YPRMEDFWRDLAAVYAREIAELGKRGCTYLQLDDTSLAYLNDPKQRDYVSSIGGDPNE
                     QHRIYIRNINAALSGKPAGMRVCTHMCRGNFRSAWAAEGGYDHVAEALFNELDVDGFF
                     LEYDDARSGGFEPLRFVPRNKFVVLGLVTTKRGALETKDDLKRRIDEAARFVPIEQLC
                     LSPQCGFSSTEEGNALSLDDQMAKLALVVETAREVWE"
     gene            4738..>5301
                     /locus_tag="VIV63_03050"
     CDS             4738..>5301
                     /locus_tag="VIV63_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6123603.1"
                     /translation="MKTKPVVRPIGLAIAAALSSGAFAQEQDVQRLEEITVTARKVQE
                     DLQKTPIAITALTGSALEDRQIFRTDVLDQVVPNLQFANNAPLAGNNSSSQVFIRGIG
                     QTDPTSTVDPGVGLYVDDVYIGNAVGASMALRDVANVQVLRGPQGTLFGRNTIGGAVL
                     ISTTDPGEDFGGEVRAELGNDSLLDAMV"
BASE COUNT          988 a         1766 c         1628 g          919 t
ORIGIN      
        1 cggccctggg ctcgcggatc ggcatttcta tccactctac gagaagatgt gcgagctcgg
       61 cgtgcccgcg atgatccacg tgagcgaggc ctgcaacacc ccgtatttcc aaacgacggg
      121 gtcgtactac ctcgcggcag ataccgtcgc cttcatgcag atgatgacgt cggatctgtt
      181 caaggacttc ccagacctca agctcatcat cccgcacggc ggcggcgccg tgccgtttca
      241 ttggggccgt ttccggggtc tcgccgacat gctgaagcgg ccgccgctcg cggagtcggt
      301 gctcggcaac gtgtacttcg acacgtgcgt gtatcaccag ccgggcatcg acctgctcgt
      361 gaaggtgatc gaccccgcga acatcctgtt cggttcggaa atgctgggcg ctgtgcgcgg
      421 catcgacctt gagaccggct ttcacttcga cgacacgcgt cgttacatcg acgcgctgac
      481 ggttcccacc gacaccaagc gccggatctt cgagctcaat acgcgccgcg tgtatccacg
      541 gctggacgcg caactcaagg cccggggcct atgagccaat tccagtcaga tcccggttgg
      601 ctcagctacc acgcgtctcc gcgcaagccc ggctttgttc cgccgccggg cgccgtggat
      661 gcgcactgcc acgtgttcgg ccctggcgat gtatttccct acgcgcccga acgcaagtac
      721 acgcccaccg atgcgccgaa ggaaaagctc tgggcattgc gcgatttcct cggcttcgag
      781 cgcaacgtga tcgtgcaggg cacctgccac ggcgccgaca atcgcgcgct gaccgatgcg
      841 ttgcgcagct cgggcggccg cgcacgcggt gtcgcgagcg tcaagcgcga tatcaccgac
      901 agccagctcg ccgatctgca cgacgccggc gtccgcggcg tccgcttcaa cttcgtcaaa
      961 cggctcgtcg atgtcacgcc acccgcggtg ctgctcgagg tcgcgcagcg catcgcgcca
     1021 ctcggctggc acgtggtcgt gtacttcgaa gcgcaggatc tgccggaact cgcgccgttc
     1081 atcgcctcca ttcccacgat cgtggtcgtg gatcacatgg gcaggcccga cgtgacactg
     1141 cgtcccgacg gtccggagtt cgagcgcttc gtcggattca tgcgcgagca cgccaacgtg
     1201 tggtccaagg tcagcggcgc cgagcggctg tcggcgatcg gaccgcctga ctactccgac
     1261 ttcgtgcctt tcgcgcgccg catcgtcgag acctttccgg agcgcgtgtt gtggggcacg
     1321 gactggccac atcccaacat gaagtcgcac atgcccgacg atggcgacct cgtgaacctg
     1381 ctgccgcgca tcgcgccgac ggccgaacta caacgatccc tgctggtgga caacccgatg
     1441 agactgtact ggtgaaaagt tacgctgaca ttccgggcac gacggtgttc gacgccgagc
     1501 agtcgcgcaa gggctaccac ctcaacatgt tctgcatgtc gctcatgaag gcgccgaacc
     1561 gcgagcggtt caaggcgaat gagcgcgcct acctcgacga gtggccgatg accgaagatc
     1621 agaagctggc cgtgattgcg cgcgactaca accgcatgat ctcgctcggc ggcaacatct
     1681 acttcctcgc caagatattc gccaccgatg gaaaaagctt ccagcatgcg gcggcgctca
     1741 tgaccggcat gagccaggaa gactacgcac gcatgatgct gaacggcggc cgcgcgtatg
     1801 tgccgaagga gggtgtgtaa tggcgcgcat caccgccggc gtaagctgct cgcacgtgcc
     1861 cgccatcggc gcggccatcg atctcggcaa aggacaggag ccgtactggg cgccgctgtt
     1921 cgccggcttc gagttttcga agaagtggat ggcggcgaac acgccggatg tggtgttcct
     1981 cgtctacaac gatcacgcca cggcgttcag cctcgacttc atcccgacgt ttgcgatcgg
     2041 ctgcgccgcc gagttcacgc ccgccgacga aggctggggc gcacgccccg tgccggtggt
     2101 gaaaggcgcg ccggaactcg ccgcgcacat cgccgcgagc gtcatcgaat ccgatttcga
     2161 cctgacgctc gtcaacaaga tggacgtgga ccacggcctg acggtgccgc tctcgctgat
     2221 gttcggccag ccgaaggcct ggccgtgcct ggtgattcct ttcgcggtaa acgtcgtgca
     2281 gtacccgccg ccgtccggca atcgctgcta tcaactcggc aaggccatcc gccgagcggt
     2341 agatagcttc ggccaggatc tcaacgtgca ggtctggggc acgggcggca tgagtcacca
     2401 gctccaggga ccgcgcgccg gcctcatcaa caaggacttc gaccacaaat tcctggacgg
     2461 catggtcagg gatcccgagg cgctcacgcg gatcccgcgc gagcaatacc tgcgcgaagc
     2521 gggttcggaa ggcatcgagc tcgtgatgtg gctgatcatg cgcggtgcgc tcgaagggcc
     2581 ggtccgcgaa gtccatcgtt tctatcacgt gcctgcgtcg aacaccgccg tgggccatct
     2641 catcctggag ccggcatgac tcagccgatc aagattgtcg tcgccggtgc cggcgccttc
     2701 ggtcagaaac atctcgatgc cgtgaagctc atcgacggca tcgaagtggc gtcgatcgtg
     2761 ggacgcgaac tggcgcccac cgaagagatc gcgcgcaagt atggcgtcgc tcatgccacg
     2821 acggatctcg ccgaggcgct ggcgcgcccg ggagtcgagg ccgcgatcct ctgcacgccg
     2881 acgcagttgc acgccgcgca gacgattcag tgcctgaacg ccggcaaaca cgtgcaggtg
     2941 gaaattccgc tcgcggattc cctggccgat gccgagaccg tcgcccggct tgcaaagagc
     3001 agcgggctcg tctgcatggt cggtcacacg cgccgcttca atccttcgca tcaatgggtt
     3061 catcgccgca ttaccgcggg cgagatcgcc atccagcaga tggacgtgca gacgtatttc
     3121 ttccgccgca ccaacatgaa tgcgctcgga cagccacgct cgtggacgga tcacctgctg
     3181 tggcaccacg ccgcgcacac ggtggatctg ttccgctatc agacgggctc tgagatcgtg
     3241 cgcgcgaacg cattgcaggg gcctgttcac ccgacgctcg gcatcgccat ggacatgtcc
     3301 atccagctgc tcgcggccaa cggcgccctc tgcacgttgt cgctgtcatt caacaacgac
     3361 ggtccgctgg gaacgttctt tcgctacatc tgcgaccgcg gcacctacat cgcgcgttac
     3421 gacgaactca tcgacggcaa gaacaacgcg atcgacgttt ccaaagtgga cgtctccctc
     3481 aacggcatcg agctgcagga ccgcgagttc ttcgccgcga tccgcgagaa gcgcgaaccg
     3541 aattcgagcg tggcgcaagt gctgccttgc taccggactc tgaacgacct ggagaaacag
     3601 ctgtgccgct gactcaaccg ccattccgtg ccgatcacgt gggcagcctg ctgcggccgc
     3661 catcgctgac acgcgcgcgt gccgagcaca aggccggccg catcgacgac gtggcgctga
     3721 aagccgccga ggacgaggcg attctcggcg ccgtacgcat gcaggaaaaa ctgggcctgc
     3781 aaggcatcac cgatggcgag atgcgccgct cgtcctggca catggatttc ctctaccaga
     3841 tcggtggggt gcgcaagatc cagcagaaca tcaccgtgca ttttcacagc gacaagggtg
     3901 atgtcgattt cacgccgtca ggactgcgcg tggaaggcaa gctcgggctc gacggctgca
     3961 tcttcggcga tcacttcgac tatctgaagt ccgtcgtcac acgtggcacg cccaagctca
     4021 cgattccgtc gcccagcatg atgcactacc gcggcggccg cgccgcgatc gacgagacca
     4081 tctatccgcg catggaagat ttctggcgcg acctcgctgc cgtgtacgcg cgtgagatcg
     4141 cggaactcgg caaacgcggc tgcacctatc tgcagctcga cgacaccagc ctggcctatc
     4201 tcaacgatcc gaagcagcgc gactacgtct cgagcatcgg cggcgacccg aacgagcagc
     4261 accgcatcta catccgcaac atcaacgcgg cgctctccgg caagccggcc ggcatgcgcg
     4321 tgtgcacgca catgtgccgc ggcaacttcc gctccgcatg ggccgccgag ggcggctacg
     4381 accacgtggc cgaagcgctg ttcaacgaac tcgacgtcga tggcttcttt ctcgaatacg
     4441 acgacgcgcg ctcgggcggt ttcgagccgc tgcgtttcgt gcccaggaac aagtttgtcg
     4501 tgctcggcct cgtgaccacc aaacgcggcg ccctcgaaac caaagacgat ctcaagcgcc
     4561 gcatcgatga agcggcgcgt tttgtaccga tcgagcagct gtgcctcagc ccgcaatgcg
     4621 gcttctcgtc gacggaagag ggcaatgcgc tctcgctgga tgatcagatg gccaagctcg
     4681 cgctcgtcgt ggagaccgcg cgcgaagttt gggagtagtt cacaaccggg gggaaacatg
     4741 aagacgaaac cagtagtcag gcccataggg cttgcgatcg cggctgcgct ctcgtccggc
     4801 gcattcgcgc aggaacagga cgtgcagcgt ctcgaagaga tcaccgtcac cgcacgcaag
     4861 gtccaggaag acctgcagaa aacgccgatc gcgatcaccg cgctcacggg cagcgcgctc
     4921 gaagaccgcc agatattccg caccgatgtg ctggaccagg tcgtgcccaa cctgcagttc
     4981 gccaacaacg cgccgctcgc gggcaacaac tcgtcctcgc aggttttcat ccgcggcatc
     5041 ggtcagacgg atcccacctc cacggtcgac cccggtgtgg gcctgtacgt cgacgacgtg
     5101 tacatcggca atgccgtcgg cgcgtcgatg gcgttgcgcg acgtcgcgaa cgtgcaagtc
     5161 ctgcgcggtc cgcagggaac cttgttcggc cgcaacacca tcggcggtgc ggtgctcatc
     5221 tcgaccaccg acccgggtga ggatttcggc ggcgaagttc gcgcggaact cggcaacgac
     5281 agtctgctgg acgccatggt c
//
LOCUS       DATDEU010000123         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_168265, whole genome shotgun sequence.
ACCESSION   DATDEU010000123 DATDEU010000000
VERSION     DATDEU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168265"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..>2624
                     /locus_tag="VIV63_03055"
     CDS             121..>2624
                     /locus_tag="VIV63_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123604.1"
                     /translation="MVRGVRGAQEAAIEIKRKTTEITDSIVMEDIGKLPDVTITDALQ
                     RVPGIQISRAAGEGSFLSIRGAPQIMATMNGERFVTAENILDSTVSFEDVPADQITGV
                     TVYKSQSANLTDGGLGGLLDLQSIRALKLPGEGLTLAGAAQAGWGSIADGVDQKYSGL
                     VGFKFNDRLAMALSGSFSDSAAASSFQSAELDLVDEFSGWINPGNVPSGNINGDGDAS
                     DEFLIPNGWNTSVNSREYSRDRLGLAYNFDMELSDSMELIADVFYNDMDEAQHGQQLF
                     VNGNFGGRQVFRPYTVATGQPSVLDSFDTDGLASRANFATDFIGYTNGLRGGVQSVFR
                     ETTALNTNLEFRWGKGERFSSTVRWVHADAERESRALTVAQQTDTQCFPTTQAQADAD
                     GCTDINPSAIPTTLTYEISNSLGSDQMNYVIGDQLRNLASSPSAWRMHSSWLERNATD
                     TNMDVLRYDGKLAFSENLSIDFGLRLSEREMNEARSDYFSPSGLNGLLAKYQEVGYGI
                     NQTSNTAGFDYDPLPRYTLASPELAGFVTTVGEFGVNGLVTGFPMINTRALEHPESIR
                     DALYGEGQYIDAPDRTYGVEEAQTSFYFMADFNSELTDAVDISGNVGVRVVNTNIRVF
                     QNVTDGTQLDPRILAGSDPNHTAYTDLGDVYTTTDRTRALPSFNLNFDFNNGMKLKTA
                     YYETQALQPLQNLGRGPITFYNGEQIGETWQRVSSIQRLGNPRLEPWLSRSLSTNLEW
                     YFREHSLLSAGVFYTEVDSYTFTRIGTDPTAPDSDGVVRLGAQTLDIAQGEGASYYGF
                     EFLYKATFDFLPGFLANTGIDLNYTFTPSQAGKDAST"
BASE COUNT          535 a          840 c          767 g          482 t
ORIGIN      
        1 ggtcgtcgcg ctgcgcgcgc cgcattcgta acggcggttt ctctggatct tctggccatt
       61 gccgcgccag tccatgcgca ggatcaggcg gcgtccgcag cggacgcact cgaagagatc
      121 gtggttcgtg gtgtccgtgg cgcacaggaa gccgccatcg aaatcaagcg caagacaacc
      181 gagatcaccg attccatcgt catggaagac atcggcaaac tgccggacgt caccatcacc
      241 gacgcgttgc agcgcgttcc cggcatccag atctcgcgtg ccgcgggaga aggctcgttc
      301 ctgtcgattc gcggcgcgcc gcaaatcatg gcgaccatga acggcgaaag attcgtgacc
      361 gccgagaaca tcctcgacag cacggttagc ttcgaagacg ttccggccga tcagatcacc
      421 ggcgtcacgg tctacaaatc gcagagcgcc aacctcaccg acggcggtct cggtggtttg
      481 ctcgatctgc agtcgatacg cgcgctcaaa cttccgggtg aaggcctgac gctcgccgga
      541 gccgcgcaag cgggctgggg cagcatcgcc gacggtgtcg accagaaata cagcggcctc
      601 gtgggtttca aattcaacga tcgcctcgcc atggcgttgt ccgggtcgtt cagcgacagc
      661 gccgctgcga gctctttcca gtccgccgaa ctcgacctgg tcgatgagtt ttccggctgg
      721 atcaaccccg gcaacgtgcc gagcggcaac atcaacggcg acggtgatgc ttccgacgag
      781 ttcctgatcc ccaacggctg gaacacttcg gtgaactccc gcgagtattc gcgcgaccgc
      841 cttggcctcg catacaactt cgacatggag ctgtccgact ccatggagct gattgccgat
      901 gtgttctaca acgacatgga cgaggcgcag cacggccagc agttgttcgt gaacggaaac
      961 ttcggcgggc gccaggtatt ccgtccttac acggttgcaa cgggacagcc ttcggtactc
     1021 gactctttcg acaccgacgg actcgccagc cgggccaatt ttgcgacgga cttcatcggc
     1081 tacaccaacg gcctgcgcgg cggcgtgcag tcggtgttcc gcgaaaccac cgcgctcaac
     1141 accaacctgg aattccgctg gggcaagggc gaaaggttct cgagcacggt gcgctgggtc
     1201 catgcggatg cagagcgcga atcgcgcgcg ctcaccgtgg cccaacagac cgacacgcag
     1261 tgttttccca ccacgcaggc gcaggccgac gcggatggat gcacggacat caatccgtcg
     1321 gccatcccga cgacgctgac ctacgagatc tcgaacagcc tgggttccga tcagatgaac
     1381 tacgtgatcg gcgatcagct tcgcaatctc gcttcatcgc cgagcgcgtg gcgcatgcat
     1441 tcgagctggc tcgagcgcaa cgccacggat accaacatgg atgtgcttcg ctacgacggc
     1501 aagctggcct tctccgagaa cttgtcgatc gacttcggtc tgcgtctatc cgagcgcgag
     1561 atgaacgaag cccgctctga ctacttttcg ccgtcgggcc tgaatggcct gctcgcgaag
     1621 taccaggaag tgggatacgg catcaaccag acctccaaca ccgccggctt cgactacgac
     1681 ccgctgccgc gttacacgct cgcgtcgcca gagctcgcgg gcttcgtgac gacagtcggg
     1741 gaattcggcg tcaacggcct ggtcacggga ttccccatga tcaacacgcg cgcgctggag
     1801 catccggaat ccattcgcga tgcgctgtat ggcgaaggtc agtacatcga tgcaccggat
     1861 cgaacttacg gagtcgaaga agcgcagacc agcttctact tcatggctga tttcaattcg
     1921 gaactgaccg atgcggtgga catctccggc aacgtcggtg tgcgcgtggt caataccaac
     1981 atccgcgtat tccagaacgt caccgacgga acgcagctcg atccgcgcat cctcgcgggc
     2041 agcgatccga accatacggc gtacacggac ctgggtgatg tctacacaac gaccgatcgc
     2101 acgcgcgcac tgccgagctt caacctgaac ttcgatttca acaacgggat gaagctgaag
     2161 accgcgtact acgaaacgca ggcgctgcag ccattgcaga acctcgggcg tggtccgatc
     2221 accttctaca acggcgagca gattggtgag acctggcagc gtgtaagcag catccagcga
     2281 ctcggcaatc cgcgcctgga gccatggctg tcgcgttcac tctccaccaa cctggagtgg
     2341 tatttccgtg agcacagcct gttgtcggcg ggtgttttct acaccgaggt cgacagctat
     2401 acgttcacgc gcatcggcac cgaccccacc gcgccggatt ccgatggcgt ggtgcgactc
     2461 ggcgcacaaa ccctggatat cgcgcagggt gaaggcgcga gctattacgg cttcgagttc
     2521 ctctacaagg ccacgtttga tttcctgcca gggttcctcg cgaacacggg catcgatctc
     2581 aactacacct tcacgcccag ccaggcgggc aaggatgctt cgac
//
LOCUS       DATDEU010000124         2998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1189790, whole genome shotgun sequence.
ACCESSION   DATDEU010000124 DATDEU010000000
VERSION     DATDEU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189790"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VIV63_03060"
     CDS             <1..627
                     /locus_tag="VIV63_03060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY6123605.1"
                     /translation="YPDHAVLAEESGASGDNDVVWIIDPLDGTTNFMHGFPTFAVSIA
                     CQIRGRLEHAVVFDPMRQEVFTASRGDGAQCEGRKIRVSKQVTLEGSLIATGFPYRTD
                     APYLDEYLAMLKAVMLKAAGLRRPGAAALDLAYVAAGRVDGFWEIGLKPWDTAAGTLL
                     ITEAGGRIGTIFGEEYKQGGHVIAGTAKVYAALVAELSPHAPASLREA"
     gene            complement(624..2444)
                     /locus_tag="VIV63_03065"
     CDS             complement(624..2444)
                     /locus_tag="VIV63_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6123606.1"
                     /translation="MGWRTKSIAQVQSEFDRGELKRTLGPIHLILFGIGCIIGAGIFV
                     RTGTAAALYAGPAVMLSFVIAGVVCAFAGLCYAELASMLPVSGSAYTYAYTTVGEFGA
                     WIMGALLLLEYALAASVVAVGWSGYAVSLLNDIGLFIPAELTQAVGKLVVDKASTFSM
                     TDPLVSLNGVTATLTDGSVVQFLSQGKATISGAFSTALAQYDVVDQAKAGGYVLINSL
                     DIAVNANSQAVLTEATKVMDPATAHLRDLAVGTQLSVPAGSIVQLPLATVVPVDPSSI
                     VTSLFNLPAVFITLSVTMLLVLGVSESALVNNIIVAIKVTVILAFIAVGAFFVNPDNW
                     HPFIPEPTGVENQFGMDGVIRAATIVFFAYIGFEAVSTAGQEAKDPRKDMPIGILGSL
                     IICTVLYMLTAAVLTGVVSFTKLNVAAPVATAVDTFGPEWGWLAKLIKIGAIAGLSSV
                     VLVLMFGQTRVFYAMSRDGLLPKTLARVHPKFKTPWINTLIVGVLVSLAAAVYDINKL
                     GDLTSVGTLVAFGLVCFSVIWLRFKRPDLPRHFKVWGYPVIPAIGVILCFVLAWIGVE
                     HAIRVWFAGFLVVTVAAYFLYPFWVSPMRNKAPDATTPAS"
     gene            complement(2581..>2998)
                     /locus_tag="VIV63_03070"
     CDS             complement(2581..>2998)
                     /locus_tag="VIV63_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY6123607.1"
                     /translation="AEEERKTRVFANFTAIVVQIAIIDIVFSLDSVFTAVGLATPDQL
                     PIMVGAIVLAIGVMMWVSGSISAFIERHPTIKILALAFLILVGVVLIAESFHFDVPKG
                     YLYFAMAFSVGVEMVNIRLRRLMDARRHRKDEEQGG"
BASE COUNT          591 a          942 c          986 g          479 t
ORIGIN      
        1 tatcccgacc acgccgtcct cgccgaggaa agcggcgcct cgggcgacaa cgacgtggtg
       61 tggatcatcg acccgctcga cggcaccacg aacttcatgc acggcttccc gacgttcgcc
      121 gtgtccatcg cctgccagat ccgcggccgg ctggagcacg cggtggtgtt cgacccgatg
      181 cgccaggaag tcttcacggc ctcgcgtggc gacggcgccc aatgcgaagg ccgaaagatt
      241 cgcgtcagca agcaggtcac gctcgaaggc tcgctgatcg ccaccggttt cccgtaccgc
      301 accgatgcgc cctatctcga cgaatatctc gcgatgctca aggccgtgat gctgaaggcc
      361 gcggggctgc gccgtcccgg cgcggcggcg ctcgatctcg cctatgtcgc ggcgggccgc
      421 gtcgatggat tctgggaaat cggcctgaag ccgtgggaca cggccgcggg cacgctgctc
      481 atcaccgaag ccggcggccg catcggcacc atcttcggcg aggagtacaa acagggcggc
      541 cacgtgatcg cgggcaccgc caaggtctac gcggcgctgg tggcggagct ttcgccccac
      601 gcaccagcga gtcttcgcga agcctaacta gcgggcgtcg tggcgtccgg ggccttgttg
      661 cgcatcgggc tcacccagaa cgggtagagg aagtacgccg cgaccgtcac cacgaggaat
      721 ccggcgaacc acacgcggat tgcgtgttcg acgccgatcc atgcgagcac gaagcacagg
      781 atgacgccga tcgcgggtat cacgggatag ccccacacct tgaagtggcg cggcagatcg
      841 ggccgcttga agcgcagcca gatcaccgag aaacacacca ggccgaaggc aaccagcgtt
      901 ccgaccgacg tgaggtcgcc gagcttgttg atgtcgtaca cggcggccgc cagtgacacc
      961 agcacgccga cgatgagcgt gttgatccac ggtgtcttga acttcggatg cacgcgggcg
     1021 agcgtcttcg gcaacaggcc gtcgcgcgac atggcgtaga acacgcgcgt ctggccgaac
     1081 atgagcacca gcaccaccga cgacaggccg gcgatcgcgc cgatcttgat cagcttcgcc
     1141 agccagcccc actcgggacc gaaggtgtcg accgccgtcg ccaccggagc ggccacgttg
     1201 agtttcgtga acgacacgac acccgtgagc acggcggccg tgagcatgta gagcacggtg
     1261 cagatgatca gcgagcccag gatgccgatg ggcatgtcct tgcgggggtc cttggcttcc
     1321 tggcccgcgg tcgagaccgc ctcgaagccg atgtacgcga agaacacgat ggtggccgcg
     1381 cggatcacgc catccatgcc gaattgattt tcgacgcccg tgggttccgg gatgaacgga
     1441 tgccagttgt cggggttcac gaagaacgca ccgaccgcga tgaacgcgag gatcaccgtg
     1501 accttgatgg cgacgatgat gttgttgacc agcgccgact cggagacgcc cagcaccagc
     1561 agcatggtca ccgacaacgt gatgaacacc gccggcagat tgaacagcga ggtcacgatc
     1621 gaagacgggt ccaccggaac cacggtcgcg agcggcaact gcacgatgga cccggccgga
     1681 accgagagtt gcgtgccgac cgccaggtcg cgcaggtgcg cggtggcagg gtccatgacc
     1741 ttggtggctt ccgtcagcac ggcctggctg ttggcattga cggcgatatc gagtgagttg
     1801 atgagcacgt atccaccggc cttggcctgg tcgacgacgt cgtactgggc cagcgccgtg
     1861 gagaacgcgc cggagatcgt cgccttgcct tgcgacagga attgcaccac cgagccatcg
     1921 gtgagcgtgg ccgtcacgcc attcaggctg accagaggat cggtcatgct gaacgtcgaa
     1981 gccttgtcga ccacgagttt gcccacggcc tgcgtgagct cggcgggaat gaagagtcct
     2041 atgtcattca gcagacttac cgcgtaaccc gaccatccca ccgccactac cgaggcggcg
     2101 agcgcatatt cgagcagcag cagcgcgccc atgatccacg cgccgaattc gccgaccgtg
     2161 gtgtacgcgt aggtgtaggc ggagccggag accgggagca tcgaggcgag ctcggcgtag
     2221 cagagtccgg cgaaggcgca gacgacgccc gcgatgacga aggacaacat gaccgcaggt
     2281 cccgcgtaca aggcggccgc ggtgccggtt cgaacgaaga tgccggcgcc gatgatgcag
     2341 ccgatgccga agaggatgag gtgaatcggt ccgagtgttc gcttgagttc gccgcggtcg
     2401 aactcggact ggacctgcgc aatcgacttg gtacgccaac ccatcgggga ccccgcaatg
     2461 ttgttattgg ccggattgta gggacgcagc gcaggttaag cgagtgattg ccgtccattt
     2521 agatgtacgg cgccgcttcg gtgccggttt ccgaagtggc gcgtgagcgc ctggcaataa
     2581 ttacccgccc tgctcttcgt ctttccggtg ccggcgcgca tccatgaggc gtcgcagccg
     2641 gatattcacc atttcgacgc ctaccgagaa cgccatggcg aagtacaggt aacccttcgg
     2701 cacgtcgaag tggaagcttt cggcgatcag caccacgccg accagaataa ggaatgccag
     2761 cgcgaggatc ttgatggtcg ggtgacgctc gatgaacgcg ctgatggacc ccgacaccca
     2821 catcatcacg ccgatcgcga gaactatggc gccgaccatg atgggtagct gatcgggcgt
     2881 cgcgaggccg accgccgtga acaccgagtc gagcgagaac acgatgtcga tgatggcgat
     2941 ctgcacgacg atggcagtga agttggcgaa gacgcgcgtc ttgcgttcct cttccgcg
//
LOCUS       DATDEU010000125         3081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1482020, whole genome shotgun sequence.
ACCESSION   DATDEU010000125 DATDEU010000000
VERSION     DATDEU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482020"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VIV63_03075"
     CDS             complement(<1..662)
                     /locus_tag="VIV63_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123608.1"
                     /translation="MKTLIALFAFMQGAAIAPAPKPADFPQQKQLAANVYVWSDIHPS
                     GLYTTNNLIVVTTDGVLVADGQKDADTTKKMVEGIAKLTKQPIKVVIIGSEHGDHTGG
                     NASFPSTVTYIKSPLSSGKETTLRMGNTQIQVLDRGRAHTGTDLEVYLPQEKILFASE
                     AYSNHIFPNMRAAVPAEWIQTVKALRAIDANTVIPGHGAIEDGPAMKASLAAFEQSLE
                     YIV"
     gene            complement(664..1845)
                     /locus_tag="VIV63_03080"
     CDS             complement(664..1845)
                     /locus_tag="VIV63_03080"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY6123609.1"
                     /translation="MHSTITHLQCSRGTCAKVVPHTTLQNLCPACKSPLLARYDLDAA
                     RQTLSLDALRTRPNTMWRYEEVLPGGPAITLGEGMTPLIHANRLGGRIGLPRFYIKDE
                     GLNPTASFKARGLSAAVTAAKVLGARTLALPTAGNAGGAAAAYAAKAGLACVIAMPAD
                     TPKANIIESRAFGADVRLIDGLISDCGKFIAEHAAREGWFEVSTLKEPYRIEGKKTMG
                     YELWEEFGGKLPDVIVYPTGGGVGLIGMWKAFEELEAMGFIGGERPRMISAQAAGCAP
                     IVQAFEQHRDSSDFFENASTVASGLRVPKPLGDFLILADLYASGGEAVPATDDEMLAA
                     CRDMAAFEGIFAAPEGGAGLVATTKLVKAKKIHDTDTVVLFNTGAGYKYLEAWQAALE
                     N"
     gene            1932..2777
                     /locus_tag="VIV63_03085"
     CDS             1932..2777
                     /locus_tag="VIV63_03085"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEY6123610.1"
                     /translation="MSDELANFDLEKAGAPGDVPPPPPPPTRNQLPVAALIVAAVLLF
                     GLTAAYVFLRRPASETPAPNVTKPPPAPAVQEQITLPPLDETDAVVRDLVRKLSSNPV
                     VAAWLTTDSLILNFVAVTSRIANGVTPVAELKSIGPVPRFATRTVRNSTYIDRASYRR
                     YDRYAQAVAALDARGVAETYNTLKPRVKEGYGRLTSGDDSFDPVLEGAIVELLRVPVV
                     DGEVELYPHGTVWAFADPQLQELSAAQKQLLRMGPQNVRTVQSKLREIATALGIPASR
                     LPPVK"
     gene            complement(2788..>3081)
                     /locus_tag="VIV63_03090"
     CDS             complement(2788..>3081)
                     /locus_tag="VIV63_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY6123611.1"
                     /translation="MGRDARESGLRAVFGTHDARLINAIQRHMDSTGEPRESAEFHLL
                     FGIQRAEQARLAREHYRARILVSYGEQWFPWYMRRLAERPANVLFVAKSMLSK"
BASE COUNT          524 a          956 c          996 g          605 t
ORIGIN      
        1 acgatgtatt caagcgactg ttcgaaggcc gcaagcgagg ccttcatcgc gggaccgtcc
       61 tcgatcgcac cgtggccggg tatcacggtg ttcgcgtcga tggcgcgaag ggccttgacc
      121 gtctgaatcc actccgcggg caccgctgcg cgcatgttgg gaaagatatg gttcgagtag
      181 gcctcgctcg cgaacagaat cttctcttgc ggcaggtaca cctcgaggtc ggtgccggta
      241 tgcgcgcggc cgcggtcgag cacctgaatc tgcgtattgc ccatgcgcag cgtcgtctcc
      301 ttgccgctgg agagaggact cttgatgtac gtgacggtcg acgggaacga cgcgttgccg
      361 cctgtgtgat cgccgtgctc agatccgata atcaccacct tgatgggctg cttcgtcagc
      421 ttcgcgatcc cctcgaccat tttcttcgtc gtgtccgcat ccttctggcc gtctgccacg
      481 agcacgccgt cggtggtgac gacgatcaga ttgttcgtgg tgtagaggcc cgacgggtgg
      541 atgtctgacc agacatatac gttggccgcg agctgtttct gctgcggaaa gtctgccggt
      601 tttggcgcgg gcgcaatcgc ggcgccctgc atgaacgcga aaagagcgat cagggtcttc
      661 atgtcaattc tccaatgcgg cctgccacgc ctcgaggtac ttatatccgg cgcccgtgtt
      721 gaacagcacg accgtgtcgg tgtcgtgaat cttctttgcc ttgacgagct tggtggtcgc
      781 caccaggccg gctccccctt cgggcgccgc gaagatgcct tcgaaggcgg ccatgtcgcg
      841 gcacgccgcg agcatttcgt cgtctgtcgc tggaaccgcc tcgccgccgc ttgcgtacag
      901 atccgcgagg atcaggaaat cgccaagcgg cttcggcaca cggaggccgg atgcgacggt
      961 tgaggcgttt tcgaaaaagt cggagctgtc gcggtgttgc tcgaaggcct gaacgatcgg
     1021 cgcgcaaccg gcagcctgcg ccgagatcat gcgcgggcgc tcgccgccga tgaagcccat
     1081 ggcttcgagc tcttcgaacg ccttccacat gccgatcagg ccgacgccgc cgccagtggg
     1141 gtagacgatc acatctggca gcttcccgcc aaattcttcc cacagctcgt atcccatcgt
     1201 ttttttgcct tcgatgcggt acggctcctt gagcgtagag acttcgaacc agccctcccg
     1261 ggcagcgtgt tcggcgatga acttgccgca gtcggagatc aggccgtcga tcaaccgcac
     1321 gtcagcacca aaggcgcgag actcgatgat gttcgctttc ggtgtgtcgg ccggcatcgc
     1381 gatgacgcat gccaagcccg ctttcgcagc gtaggccgct gcggctccgc cggcattccc
     1441 ggctgtcggc agtgcgagcg tccgtgcacc gagtaccttc gcggctgtga ccgcagccga
     1501 cagcccgcgc gccttgaacg acgcggtcgg gttcaggcct tcatccttta tatagaaccg
     1561 gggaagaccg atccgcccgc cgaggcgatt ggcgtgaatc agcggcgtca tcccctcacc
     1621 gagcgtgatg gccggcccgc caggcagcac ttcttcatag cgccacatcg tgttggggcg
     1681 tgttcggagc gcgtcgaggc tcagcgtctg ccgcgcggca tccaggtcgt agcgcgcaag
     1741 caagggggac ttgcaggcag ggcagaggtt ctgcaacgtc gtatggggca caacttttgc
     1801 gcacgtaccg cgcgagcatt gaagatgtgt gattgtcgag tgcatgagag ggagagtcta
     1861 gcagggctga gtccggaaaa tcaggtgcac ccgcgatggc ataagtcagg ccggtgtggg
     1921 atcctgtgct catgagtgac gagctggcaa atttcgatct cgagaaagcc ggtgctcctg
     1981 gtgacgtgcc gccgccgcct ccgccaccga ctcgaaacca gttgcccgtg gctgcactaa
     2041 ttgttgccgc cgttctgctg ttcgggctca ccgccgcgta cgtgtttttg cgccggcccg
     2101 cgtcggagac gccggcgccg aatgtgacga agcctccacc tgcgccggct gtgcaagaac
     2161 agattacgct gcctccgctg gacgagacgg atgcggttgt ccgcgacctt gtccgaaagt
     2221 tgtcgtcgaa tcccgtcgtt gcggcgtggt tgacgactga ttcgctgatc ctgaattttg
     2281 tggcggtgac atcgcggatc gcgaacggcg tcacgccggt ggccgagctg aagtccatcg
     2341 gcccggttcc gcgatttgcc accaggactg ttcggaacag cacctacatc gatcgggcga
     2401 gctaccggcg ctacgaccgt tacgcgcagg ctgtcgctgc cctcgacgcg cgaggcgttg
     2461 ccgagacgta caacacactg aaaccccgcg tcaaagaagg gtacggccgg ctcacatccg
     2521 gagacgacag cttcgatcct gtgctcgagg gcgccattgt cgagctcctc agggtgccgg
     2581 tcgtcgacgg cgaagtcgag ttgtatccgc acgggaccgt gtgggcgttt gcggacccgc
     2641 agttgcagga gttgtccgcg gcccagaagc agcttctgcg catgggtccg cagaacgtgc
     2701 gcacggtgca gagcaagctg cgcgaaattg cgacggctct cgggattccg gcgtcgaggc
     2761 tgccgccggt gaagtgatcg ggtgcgctca cttcgacagc atcgacttcg cgacgaacag
     2821 cacgttcgcc ggccgctcgg ccaggcgtcg catgtaccac ggaaaccact gttcgccgta
     2881 gctgacgagg atgcgcgcgc gatagtgctc tcgcgccaat cgcgcctgct cggcacgctg
     2941 gatgccgaag agcaggtgga actcggctga ctcgcgaggt tctcccgtcg aatccatgtg
     3001 gcgctggatg gcgttgatga ggcgcgcgtc gtgtgtaccg aacacggcgc ggaggcccga
     3061 ctctcgagcg tcacgcccca t
//
LOCUS       DATDEU010000126         4792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_606674, whole genome shotgun sequence.
ACCESSION   DATDEU010000126 DATDEU010000000
VERSION     DATDEU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606674"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2612)
                     /locus_tag="VIV63_03095"
     CDS             complement(<1..2612)
                     /locus_tag="VIV63_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY6123612.1"
                     /translation="MNARPESTVAPAEHLPAEFRSALELAQAAVWDWQVSADHFQVDE
                     VWLRALGIDMLVNAMPSAEWKRRIHPDDLGAFIAAADSCHHGGDRFECEYRLLAVGHR
                     WLWVLHRGRVVQRERDGSAQRITGLLLDIDRRKSEEVARSDSESRLATALWGARAAFW
                     QWHVPSNARTSSPLWLAMTGYSRAQWDTMPNPWFGNLHPDDRERVDRQLKEHAHGERD
                     SVEFEYRFQTAGGEYRWMQDRGRAVEWDLQGRPVLVIGVTLDIDAAKHAEAHLRSSES
                     MLETAAWGAGIGLWETNFITDKTRWFNDWCDRHDIHPCDGDDHVERWDSYLHPDEGPE
                     ATRRFSEHVAGKAEYYDAEYRIKTRKGAWRWVFERGRVVERDAQGKAVRMLGVCMDLD
                     ETKVAERRANARNERVEAALQLTTAGVWDWDVEHGVTNDTDGYYRVFGVDPAFARANI
                     HTWRQVLGTDPDREIERFRERLLHVDSAAQVLETEYRFRHTDGSWHWALDRAYVVDRA
                     PDGSTLRVLGLVVDITARKIRETGLSAADQRFRAIARELRCVIYEIDAETGLSSGEGV
                     DRVLGYSNTDLPRPGDWAALVHPDDQHLLRQWFDRGNESMVALQYRIRHRDGRYITLL
                     DSPCVVRDAAGKVVRIVGVAIDISEQANAQEALRASQELLQIVAAGTGDWLILVDTQR
                     CVQFINRDIRSHSRDSIIGRRVEEIAEPEDRPNILAALSQVLETGEPVDLQLASSPRD
                     GRIFDSRVRAVRSSNGITGAVINITEITDREAAMHLRETQARMFELLHEGVVVIDVNN
                     MIRMANPAFERMFGFTSGAAVDTAIGDLIAPTPGMLRERLDQQLFGNITEPPGLTPVE
                     FKCRRRDGSTFDAA"
     gene            2870..3667
                     /gene="gntF"
                     /locus_tag="VIV63_03100"
     CDS             2870..3667
                     /gene="gntF"
                     /locus_tag="VIV63_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis pre-guanitoxin forming
                     N-methyltransferase GntF"
                     /protein_id="HEY6123613.1"
                     /translation="MTRNPLVADYSEWKARDYFQTYYSEVVLPDEQAVLAYQIDVLRA
                     ARARFGRGLEYGCGPTMHRAIAASKYAFRIDMADWLADNLSVAREWLCADDSAPEWKR
                     FTRYVLACEGHARPSTARIVQREAQTRKVVRGLYVSDARLRQPLGPTREGFYDLVITG
                     FCIDAISSDRAVWRRCMRNVTSMLQPGGTFIIHALHQCKAYKCGDRMFPGSNLSIDDM
                     RDAMLENGFSPSSIDAQVIPCRENALYGYSGILTASGRKAPAKSRKA"
     gene            complement(3668..>4792)
                     /locus_tag="VIV63_03105"
     CDS             complement(3668..>4792)
                     /locus_tag="VIV63_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY6123614.1"
                     /translation="SPLVRRLSIALLLGLVATFSSPQHAHAHAAGTSYLILQLPEGEA
                     PVSVRWDLSMKDVVWTVFIDADYDGDVTWQEVQTAQSALGAAVLAQISVKRGPAACAL
                     SVKDFALASRGEENFLSVAMIAACPQPGLTTIGGALFMSGDASQRVLLSAGRGEEQLT
                     GVISVADAGWTEPAQASAWRSFARFIGEGVWHVLIGYDHIAFVLLLLLPSVMRPVGGK
                     WQGASGLSPVARDMVTIITAFTIAHSITLALAVTGTVKLPVRPIEIAIAASIAVAGVV
                     NLIPRLSRLRLPLAFGFGLVHGFGFANALSEIDASGSSLLPLLAGFNIGVEIAQLAIV
                     ALVLPVIYSMRATRWYANGVLPLGSCALGMAGLVWLFQRV"
BASE COUNT          851 a         1595 c         1537 g          809 t
ORIGIN      
        1 caggccgcat cgaacgtgga gccatcgcgg cgccggcact tgaattccac cggcgtgagg
       61 ccgggtggtt cggtgatgtt gccaaagagc tgctgatcga ggcgctcgcg aagcatgccc
      121 ggcgtcggcg cgatgagatc gccgatggcg gtatcgacgg ccgctcccga ggtgaatcca
      181 aacattcgct cgaaggcggg attcgccatg cgaatcatgt tgttgacgtc gatgaccacg
      241 acgccttcat gcagcagttc gaacatgcgc gcctgggttt cacgcaggtg catggccgct
      301 tcgcggtccg tgatctcggt gatgttgatc accgccccgg tgatgccgtt cgatgagcgc
      361 accgcgcgaa cgcgcgaatc gaatatgcgc ccgtcccgcg gactgctcgc gagttgcaga
      421 tccaccggct caccggtctc caggacctgg gagagcgccg cgaggatgtt cgggcgatcc
      481 tcgggctcgg cgatctcttc gacacgccgg ccgatgatgc tgtcgcgcga atgcgagcgg
      541 atgtcgcgat tgatgaactg cacgcaccgc tgggtatcga ccaggatcag ccagtcgccg
      601 gtgccggccg cgacgatctg cagcaattcc tgcgaggcgc gcaacgcctc ctgggcattg
      661 gcctgttcgc tgatgtcgat ggccacgccc acgatgcgca ccaccttgcc ggcggcgtcg
      721 cgcaccacgc acggagaatc gagcagcgtg atgtagcggc catcgcgatg cctgatgcgg
      781 tactggagcg cgaccatcga ttcgttgccg cgatcgaacc attgccgcag caggtgctgg
      841 tcgtccggat gaaccagcgc cgcccagtct cccggacgtg gcagatcggt attgctgtat
      901 ccgagtacgc ggtcgacgcc ttcaccggac gacaggccgg tctccgcgtc aatttcatag
      961 atgacgcaac gcagttcccg cgcgatcgcc ctgaaccgct ggtcggcggc cgacaacccg
     1021 gtctcgcgga tcttgcgcgc ggtgatgtcg accacgagac ccagcacgcg cagcgtcgag
     1081 ccatcgggcg cacggtccac gacgtaagcg cggtcgagcg cccaatgcca gctgccgtcg
     1141 gtatgccgga agcggtattc ggtttccagc acctgggccg cggagtcgac gtggagcaga
     1201 cgctcgcgaa acctctcgat ttcccggtcg ggatcggtgc ccagcacctg ccgccaggtg
     1261 tggatgttgg cccgggcaaa tgcggggtcg acgccgaaca cgcggtagta gccatcggtg
     1321 tcgttggtga cgccgtgttc cacgtcccag tcccagacgc ccgcggtagt tagctgcaga
     1381 gccgcctcga cgcgctcgtt gcgtgcattc gcgcggcgct cggcgacctt ggtttcgtcg
     1441 aggtccatgc acacgccgag catgcgcacg gccttgccct gggcgtcgcg ctcgaccacg
     1501 cggccgcgct cgaagaccca gcgccacgcg cccttgcgcg tcttgatgcg gtattccgca
     1561 tcgtagtatt cggccttgcc ggcgacgtgt tccgaaaagc gccgcgtggc ctcgggacct
     1621 tcatcgggat gcaggtacga gtcccagcgc tcgacgtggt cgtcgccgtc gcacggatgg
     1681 atgtcgtgcc ggtcgcacca gtcgttgaac cagcgcgtct tgtcggtgat gaaattggtt
     1741 tcccacaaac cgatgccggc gccccaggcc gcggtctcga gcatcgactc gctggaacga
     1801 agatgagcct cggcatgttt ggcggcatcg atgtcgagcg tgacgccgat gaccagcacc
     1861 ggccgtccct gcagatccca ttcgacggcg cggccacgat cctgcatcca gcggtattcg
     1921 ccacccgcgg tctggaagcg atactcgaac tccaccgaat cgcgctcgcc gtgtgcatgt
     1981 tctttgagct gtcggtcgac gcgttcgcga tcgtcgggat gcaggttgcc gaaccacggg
     2041 ttcggcatcg tgtcccattg cgcccgcgag tagccggtca tcgccagcca cagcgggctc
     2101 gacgtgcgcg cattgctcgg cacatgccat tgccagaacg cggcgcgcgc gccccacagc
     2161 gccgtggcca gccgcgactc gctgtcgctg cgcgcgacct cctcgctctt gcgccggtcg
     2221 atgtcgagca gcagtccggt gatgcgctgc gcggagccat cgcgctcacg ctgcacgacg
     2281 cggccgcgat gcaggaccca taaccatcga tggccgacgg ctaacaaccg gtattcacac
     2341 tcgaagcggt cgccgccgtg atggcaggaa tccgccgcag cgatgaatgc gccgagatcg
     2401 tcgggatgga tgcgccgttt ccattccgcg ctgggcatcg cattgacgag catgtcgatg
     2461 cccagtgcgc gcagccagac ttcatcgacc tggaagtggt ccgcggaaac ctgccagtcc
     2521 cagacagccg cctgcgcgag ctcgagcgcc gaccggaatt cggcaggcaa gtgctcggct
     2581 ggggccaccg tggattcggg acgggcgttc atgaccgatt attgttatct ctgcgacaag
     2641 cttgcacctt gatgtatctc cagctttgcg gatgccggca gtccgaaacg gggatttggc
     2701 cggctccgca tactcttatc cctgagatct cagccgctta ggcattccag taagggtcgt
     2761 acggctactg caccgcggtg gccgtcagta ttctctgaca taagcctttg acaaaaagct
     2821 tgtcagaaaa ctaacggatt agcggggtct cacagtggtt gacggaagca tgacgcggaa
     2881 tcccttggtc gctgactact ccgagtggaa agcacgtgac tacttccaga cctactacag
     2941 cgaggtcgtg ctcccggatg agcaggccgt cctcgcctac cagatcgacg tgcttcgcgc
     3001 ggcgcgtgcg cgcttcggcc gcgggctcga gtacggctgc ggccccacga tgcatcgcgc
     3061 gatcgcggca tcgaagtatg cgttccgcat cgacatggcg gactggctgg ccgacaacct
     3121 gtcggttgcg cgtgaatggc tgtgcgcgga tgacagcgct cccgaatgga aacgcttcac
     3181 gcgctacgtg ctcgcctgcg aaggacatgc gcgtccgagc accgcgcgaa tcgtgcagcg
     3241 cgaagcgcaa acccgcaaag tcgtgcgcgg cctgtacgtg agcgacgcgc gtctgcgcca
     3301 gccactcggc ccgacgcgcg aagggttcta cgacctcgtg atcaccggct tctgcatcga
     3361 cgcgatctcg agtgaccgcg cggtctggcg ccgctgcatg cgcaacgtga cctcgatgct
     3421 ccagcccggc ggcacgttca tcatccacgc gctccaccag tgcaaggcat acaagtgcgg
     3481 cgaccggatg ttcccgggct cgaacctcag catcgacgac atgcgcgatg cgatgctgga
     3541 aaacggcttc tcgccctcga gcatcgacgc ccaggtgatc ccgtgccgcg agaacgcgct
     3601 gtacggctac tcgggcatcc tcaccgcttc cggccgcaag gcgcccgcga agtcgcgcaa
     3661 ggcctgacta cactctctgg aagagccaga cgagtccggc catgccgagc gcgcatgagc
     3721 cgagcggcaa cacgccattc gcataccagc gcgtggcgcg catcgagtag atgaccggca
     3781 acaccagcgc cacgatcgcg agctgcgcga tttcgacgcc gatgttgaat ccggcgagga
     3841 gcggcagcag cgagctgccc gacgcatcga tctcgctgag cgcgttggcg aatccgaatc
     3901 catgcacgag accgaagccg aaggccagcg gcagccgcag ccgggacagc cgcggaatca
     3961 gattgaccac gcctgccacg gcgatggacg ccgcaatggc gatttcgatc ggacgaacgg
     4021 gcaacttcac cgtgccggtc accgccagtg cgagcgtgat cgaatgcgcg atggtgaagg
     4081 cggtgatgat ggtcaccatg tcgcgcgcca ccggcgaaag acccgacgcg ccctgccact
     4141 tgccacccac cgggcgcatc accgacggca gcaggagcag cagtacgaat gcgatgtggt
     4201 cgtagccgat gagcacatgc cacacccctt cgccgatgaa tcgcgcgaag ctccgccacg
     4261 cggacgcctg cgccggctcc gtccatccgg catcggccac ggagatgacg ccggtgagtt
     4321 gctcttcacc gcgcccggcg ctcaacaaaa cccgctgtga tgcgtctccg ctcatgaaca
     4381 gcgcgccgcc gatggtggtc aggccgggct gcgggcaggc cgcgatcatc gccacggaga
     4441 gaaagttttc ctcgccgcgg ctcgccagcg cgaagtcctt cacggaaagc gcacacgccg
     4501 cggggccgcg cttcacggag atctgcgcga gcacggcagc gcccagggcc gactgcgcgg
     4561 tctgcacttc ctgccaggtg acgtcgccgt cgtagtcggc atcgatgaag acggtccaga
     4621 cgacgtcctt catcgacagg tcccagcgga ccgacaccgg cgcctcaccc tcgggcaact
     4681 gaaggatgag atagctggtg cccgccgcat gcgcgtgcgc gtgctgtggc gaactgaacg
     4741 tcgcaaccag gcccaacaga agcgcgatgg atagccgtct cacgagcgga ct
//
LOCUS       DATDEU010000127         2708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1190257, whole genome shotgun sequence.
ACCESSION   DATDEU010000127 DATDEU010000000
VERSION     DATDEU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190257"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VIV63_03110"
     CDS             <1..1105
                     /locus_tag="VIV63_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6123615.1"
                     /translation="SGYYLWSETADLDPNDPVVGQESIATRIAEKLVPEVHSDSVAGT
                     RTQSDNLAARNLYLQGRYHLNQRTEEGLHKAVEFFEKAIVEDTQFSLAHSGLSDAYGL
                     LAHYGVLGPADVWAKAASCAASAVMLDGHSAEAHTSLAHVRATQDWDFSAAEPLFLRA
                     IQLNPRYSTARHWYAMSCLVPMARLDEALEQILLAQSLDPVSSIIARDVAVTHYYRRD
                     FEAALEQCDHTIELNPHFAPAYLTLGMIQEQRRDFDESAAAFRRAVDLAPNSLRMQSA
                     LARTLALSGKRERAIDALRTLAEMASTRYVSPVEFMTMAFAAGDPETGFRWLTKAGDD
                     RCFDLLALKVDPRFDGLRDDPRYAAVAGRIGLG"
     gene            complement(1147..1416)
                     /locus_tag="VIV63_03115"
     CDS             complement(1147..1416)
                     /locus_tag="VIV63_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123616.1"
                     /translation="MRGWAGIGPIRHDHPHRFAVDHQVADPLQAGTGIAANEQFLARN
                     LNYFDAIFFFGVREIDLDAQQRADLLSFRASCGTPSGCRRCTSKR"
     gene            complement(1591..2451)
                     /locus_tag="VIV63_03120"
     CDS             complement(1591..2451)
                     /locus_tag="VIV63_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY6123617.1"
                     /translation="MRSRFVFVMSLMVAAALFTLEAQGPPRGARGPQAPRSRKVVLAW
                     ADTRNGIAQHEFTSHALSVIEGLGYESGAWDTFIRTDSNIIANQPKRTTGEPASGGPS
                     LAMADAIFFLGHRDVPIDEAQQKELAAFVRDGKGFVAAHTALTAFDAWPEFGELLGGR
                     YDGHPWHAQGTVINEAADFPATRHFPPAFTINDEFYQAKAFSPNARVLLRLDVSKMPP
                     HPELRNKGFPLAWAKSYGKGRVFYSSFAHDAATWDHRDIKQMYFEAIKWALGMTEGDA
                     TPRAARPPAQ"
     gene            complement(2507..>2708)
                     /locus_tag="VIV63_03125"
     CDS             complement(2507..>2708)
                     /locus_tag="VIV63_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123618.1"
                     /translation="TTDGYFRAFDARNGAELWSTKLTVPGHSIPSTYMGKDGKQYVAI
                     TVGGGGFLRSPLSDEVVAFRLP"
BASE COUNT          474 a          884 c          880 g          470 t
ORIGIN      
        1 gagcggatac tacctgtggt cggagaccgc cgatctcgat ccgaacgatc cggtcgtcgg
       61 acaggaatcg atcgccacgc gcatcgccga gaaactggtg ccggaagtcc acagcgattc
      121 agtcgcaggg acgcgcacgc aatccgacaa cctcgccgcg cgaaatctct acctgcaggg
      181 ccgctaccat ctgaatcagc gaaccgaaga aggcctgcac aaggcggtcg agttcttcga
      241 gaaggccatc gtcgaggaca cgcagttttc cctcgcgcac agtggcctgt cggacgccta
      301 cggtctgctc gcacattacg gcgtgctcgg tcccgccgac gtgtgggcga aagcggcgtc
      361 ctgtgcggcg tccgcggtga tgctcgacgg acattcggcg gaagcccaca cctccctcgc
      421 ccacgtcagg gccacccagg attgggactt cagcgcggca gagccattgt tcctgcgcgc
      481 cattcaactg aacccgcgct actccaccgc ccgccactgg tatgcgatgt cgtgcctggt
      541 gccgatggct cgtctcgacg aagcgctcga acagatcctg ctggcgcagt cgctcgaccc
      601 ggtgtcgtcg atcatcgcca gggacgttgc ggtcacgcat tactaccgcc gggattttga
      661 agcggcgctg gaacagtgcg atcacacgat cgagttgaac ccgcacttcg cgcccgccta
      721 cctgacgctg ggcatgatcc aggaacagcg cagggacttc gacgaatcgg ccgcggcatt
      781 ccggagggcg gtcgacctgg cgccgaattc actgcggatg cagagcgcac tggcgcggac
      841 gctcgccctg tccgggaagc gcgaacgcgc gatcgatgcg ctgcgcacgc tcgcggagat
      901 ggcctcgaca cgctacgtgt cgccggtaga attcatgacg atggcgtttg ccgccggcga
      961 tccggaaacc gggtttcgct ggctcacgaa ggcgggtgac gaccgctgct tcgacctgct
     1021 cgccctcaag gtcgatccgc gattcgacgg gctgcgtgac gatccgcgat acgccgcggt
     1081 cgccgggcgc atcgggctcg gctaacgctc ccttcgactg cgctcagggc agggccatcg
     1141 accacttcag cgcttcgaag tacatcttct gcagccactc ggtgtcccac agctcgcgcg
     1201 gaacgacagc agatccgcgc gctgctgcgc atcgagatcg atctcgcgaa caccgaagaa
     1261 gaagatcgcg tcgaagtaat tcaggttgcg cgccaggaac tgttcgttgg cggcgatgcc
     1321 ggtgcctgct tgaagaggat cggcgacttg gtgatcgact gcgaatcggt gcggatgatc
     1381 gtgtcgtata ggcccgattc cagcccagcc tctcaatcgt gctcagcgcg tgggtgatgg
     1441 actcgtgctg gtagccgttg cggacatcgg cccatgccag cacatgcttg ggaaaggtcc
     1501 tcggagccgg ggattgggcc ccgaggacag tcagcgatag caacgcgagg acgacggcgg
     1561 gaaggccggc gatcctcatg ctggcgctga ctactgtgcg ggcggacggg ccgcgcgcgg
     1621 agtggcgtcg ccttcggtca tgccgagggc ccacttgatc gcttcgaagt acatctgctt
     1681 gatgtcgcgg tggtcccagg tggccgcgtc atgcgcgaac gaggaataga acacgcggcc
     1741 cttgccgtaa ctcttcgccc acgccagcgg gaagcccttg ttgcgcaact cggggtgcgg
     1801 cggcattttc gagacgtcga ggcgcagcaa cacgcgcgca ttgggtgaga acgccttggc
     1861 ctggtagaac tcgtcgttga tcgtgaacgc cggggggaag tgccgcgtcg ccgggaagtc
     1921 cgcggcctcg ttgatcacgg tgccctgcgc atgccacggg tggccgtcat aacggccgcc
     1981 cagcagctcg ccgaactccg gccacgcgtc aaatgccgtc agcgccgtgt gcgccgcgac
     2041 gaagcccttg ccgtcgcgca cgaacgctgc cagctccttc tgctgggcct cgtcaatcgg
     2101 cacgtcgcga tggccgagga agaagatcgc atccgccatc gcgaggctgg gaccgccgct
     2161 ggcgggctcg ccggtggtgc gcttgggctg attcgcgatg atgttcgagt cggtgcggat
     2221 gaaggtgtcc cacgcgccgg actcgtatcc aagaccctcg attaccgaga gcgcatgcga
     2281 ggtgaactcg tgctgcgcga tgccgttgcg ggtatcggcc cacgcaagca ccactttgcg
     2341 gctgcgcggc gcctgcggac cacgagcccc gcgcggcggc ccctgggcct cgagcgtgaa
     2401 cagcgccgcg gcgaccatca acgacatgac gaagacaaac cgtgatcgca tcatgcttcc
     2461 tgatccctgc tccctgatcc ccgatccctg atccctgatc cctgccctac ggcagccgga
     2521 acgctaccac ttcgtcactg agcggactgc gcaggaatcc gccgccgccc acggtgatgg
     2581 cgacgtattg cttgccatcc ttgcccatgt aggtcgatgg aatcgaatgt ccgggcacgg
     2641 tcagcttggt cgaccacaac tcggcgccgt tcctggcgtc gaaggcgcgg aaatagccgt
     2701 cggtggtc
//
LOCUS       DATDEU010000128         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1190401, whole genome shotgun sequence.
ACCESSION   DATDEU010000128 DATDEU010000000
VERSION     DATDEU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190401"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="ftsZ"
                     /locus_tag="VIV63_03130"
     CDS             complement(<1..999)
                     /gene="ftsZ"
                     /locus_tag="VIV63_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY6123619.1"
                     /translation="MFELMDAYSQNAVIKVIGVGGGGGNAVAHMVSSGIEGVEFLCIN
                     TDAQALKHSKVKTALQIGCNITKGLGAGADPEIGRQAAMEDRDRIIEMIEGCDMLFIT
                     AGMGGGTGTGAAPVVAQVAKELGILTVAVVTRPFEMEGGKRTLVANHGLAELGKYVDS
                     LITIPNQKLLTVLGAKTTLLDAFKSANHVLQGAVQGIAELITRPGLINVDFADVRTVM
                     SETGMAMMGSGVSRGENRAKEAAEMAVSSPLLEDINLSGAHGILVNVTAGMDLSIGEF
                     QEVGNIVKQFASDDATVVVGTVIDPELSDEMRVTVVATGLGKPEIAVSGRSPFGRDV"
     gene            complement(1054..>2085)
                     /gene="ftsA"
                     /locus_tag="VIV63_03135"
     CDS             complement(1054..>2085)
                     /gene="ftsA"
                     /locus_tag="VIV63_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY6123620.1"
                     /translation="ELMAGCEIRSVFAGIAGSHVRSLNSHGVVAIRDREVSEVDVQHV
                     VDAAKAVAIPADQKILHVLPQEFIIDGQEGIRDPIGMSGVRLEAKVHIVTGAESAANN
                     LEKCIQRCGLEVDDIVLEQLASSFACLTEDERELGVCIIDIGGGTTDLAVFSQGAIRH
                     TAVIPIAGDQVTNDIAVSMRTPTQYAEDIKVRYACALSQLANPDESIEVPSVGERPAR
                     RLARQTLAEVVEPRYEELYGLAREELRRSGFEEIIASGIVLTGGSAKMEGAIELAEEV
                     FHVPVRLGVPQHIKGLSDVVRNPIYSTGAGLLLYARDNALAGNRGKSISGNARTMAER
                     MKNWFQGNF"
BASE COUNT          345 a          690 c          639 g          411 t
ORIGIN      
        1 cacatcgcgc ccgaaaggcg agcgcccgct gaccgcgatc tcgggtttgc cgaggccggt
       61 ggccacaacc gtgacgcgca tttcatccga cagctcggga tcgatgacgg ttcccaccac
      121 gacggtggcg tcatccgagg cgaactgctt cacgatgttg ccgacttcct ggaactcgcc
      181 gatcgacagg tccatgcccg ccgtgacgtt gaccaggatg ccgtgcgcgc ccgacaggtt
      241 gatgtcctcg agcagcggcg aggaaactgc catttcggcg gcttccttgg cgcggttctc
      301 gccgcgcgac acgcccgagc ccatcatcgc catgccggtc tcgctcatga cggtgcgcac
      361 gtcggcgaag tccacgttga tgaggccggg acgcgtgatc agctcggcga tgccctgcac
      421 cgcgccctgc aacacgtggt tggcactctt gaaagcgtct aacaacgtgg tcttggcgcc
      481 gagcacggtg agcagcttct ggttgggaat ggtgatcagc gagtcgacgt atttgccgag
      541 ctccgccaac ccgtgattcg cgaccagcgt gcgcttgccg ccttccatct cgaacggacg
      601 cgtgaccacg gccaccgtga ggatgccgag ttccttcgcc acctgcgcga ccaccggcgc
      661 cgcgcccgtg cccgtgccac cacccatgcc cgcggtgatg aacaacatgt cgcaaccttc
      721 gatcatctcg atgatgcgat cgcggtcttc catcgcggcc tggcggccga tctccggatc
      781 ggcgccggcg ccgagaccct tggtgatgtt gcagccgatc tgcagcgcgg tcttcacctt
      841 ggagtgtttg agcgcctgcg catcggtgtt gatgcacagg aactccactc cctcgatgcc
      901 gctcgagacc atgtgtgcga ctgcgttgcc accgccgccg cctaccccta tgactttgat
      961 tacagcgttc tggctgtaag catccattaa ttcaaacatt gtgtcgctcc ttcactggta
     1021 aatcgaaagc tgtcagcttc gttttgaaca tcgtcagaaa ttcccctgga accaattctt
     1081 catgcgctcc gccatcgttc gcgcattacc tgaaatcgat ttgccgcgat tgcccgcgag
     1141 cgcgttgtcg cgcgcgtaca gcagcaggcc ggcgccggtc gaatagatag gattacggac
     1201 cacgtccgag agtcccttga tgtgctgcgg aacgccgagg cgcaccggca cgtggaacac
     1261 ttcctcggcg agttcgatcg cgccttccat cttggcgctg ccgccggtca gcacgattcc
     1321 cgacgcgatg atttcctcga agcccgagcg gcgcagttct tcgcgcgcca ggccgtagag
     1381 ttcttcgtag cggggctcga cgacttcggc gagcgtctgg cgcgcaaggc gccgcgcggg
     1441 acgttcgccc acggacggca cttcgatgct ctcgtcggga ttggcgagct gcgacaacgc
     1501 gcacgcgtat cgcaccttga tgtcttcggc gtactgcgtc ggcgtgcgca tcgacaccgc
     1561 gatgtcgttg gtcacctggt cgccggcgat cgggatcacg gccgtgtggc ggatggcgcc
     1621 ctgcgagaac actgcgaggt cggtggtgcc accgccgata tcgatgatgc agacaccgag
     1681 ctcgcgctca tcttcggtga ggcaggcgaa gctcgatgca agctgttcca gcacgatgtc
     1741 gtcgacttcg aggccgcagc gctggataca tttctcgagg ttgttggccg cactctccgc
     1801 gccggtgacg atgtgcacct tggcttcgag ccgcacgccc gacatgccga tgggatcgcg
     1861 gatgccttcc tggccgtcga tgatgaattc ctgcggcagc acgtgcagga tcttctgatc
     1921 cgccgggatg gccacggcct tggccgcgtc caccacgtgc tgcacgtcga cttcgctcac
     1981 ttcgcgatcg cggatggcga ccactccatg cgagttgagg ctgcgcacgt gcgaaccggc
     2041 gataccggcg aacaccgagc ggatctcgca gccggccatg agctc
//
LOCUS       DATDEU010000129        17908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_898483, whole genome shotgun sequence.
ACCESSION   DATDEU010000129 DATDEU010000000
VERSION     DATDEU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17908
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898483"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VIV63_03140"
     CDS             <1..1614
                     /locus_tag="VIV63_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6123621.1"
                     /translation="DICARKAAVFIERARAEDLVSQRDRRFQSVLESSAVPFIILSPV
                     RADTGQIVDFRFVFLNTAASIVMRVRAEEFAGRKVTDVLPRAWDDRARFHMYVDALET
                     NQVRQIERQSEADGNNNWYHIVASPLDGDLAVWFADITQRKLQERELLEADRRKDEFL
                     ATLAHELRNPLAPIRQAATIARNDAATDAQKRWSNTVIERQVQHMSLLLDDLLDVSRI
                     THGTLQLRKQQTDLQSIVSAAVETARPLIDERRHHLVVNVPDDLQVNADPLRLAQVLS
                     NLLTNAAKYTDPEGEIRVTAEQRDESLTMWVEDSGIGIATEDLPKVFGMFAQVRSAQE
                     HAAGGLGIGLALAKGIVELHGGRIEAASAGAGKGSRFTVRLPNRIVSVAPASAVDAGR
                     SMNGVSNKRILLADDNRDAAESLAILLRLEGHEVELAHDGQTALQSFAARRPDVALLD
                     IGMPKANGYEVARRIRAEPDGAGVLLIAITGWAQDADKAESRAAGFDHHLTKPIEPET
                     LMGLLDRVQEKIGTSHGGSKHPLVTEAGT"
     gene            1611..2162
                     /locus_tag="VIV63_03145"
     CDS             1611..2162
                     /locus_tag="VIV63_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123622.1"
                     /translation="MNYGPLEFANYLRRIDESQKDSATVEAARSAAPTTHPANRLTIV
                     SGGTLLTRVTRASQVEAVSVYEAVAVQAPGESGAGAVAVLVRATLRPVVLVLSSHQTV
                     HWHLALAPGAVLRAVLLSGYGESTVTGTGDAQVHSIGGFYAFRQGSAEFRHLEAEVLR
                     CTGRPIETFRSVYAGRSFEIGDD"
     gene            2431..4770
                     /locus_tag="VIV63_03150"
     CDS             2431..4770
                     /locus_tag="VIV63_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY6123623.1"
                     /translation="MKTRRMRANTAMGLALMSIAAGAADMDRTMLPIQEPKRPTYSEL
                     DARNAKPPERFQVKAPAGAPNVVIVLIDDIGFGGPSTFGGPIRTPTFDSLAANGLRLN
                     NFHTTALCSPTRVALKSGRNHHSANAGSIMETATAFPGNTGQIPNSVAPLAEMLRLNG
                     YSTGAFGKWHETAAWETSVSGPFDRWPTHQGFDKFYGFIGGETDQWYPLIYDGVKKID
                     PPKMKDYHFSVDMTNQTINWVKAQQSMTPDKPFFVYYATGAVHAPHHVPKEWADKYKG
                     QFDKGWDQIRVDTLARQKKLGLVPANTKLADRPEDLKAWKTLPEDQRRLFARQAEVFA
                     GFLEHTDDQVGRLKKALEDINEFDNTLFIYIAGDNGTSAEGGFVGMYNEMTYFNGIAE
                     KVEDLIPLIDKWGGPDTFPHMSAGWAVAFDTPFKWTKQVASDFGGTRNGVVISWPKGI
                     KEKGGLRTQFSHVIDIAPTVLEAAGLPEPKSVNGVVQIPLEGASLVYAFNDAKAPERH
                     TTQYFEMFGNRGIYSDGWFARTIHRAPWQTTNLPPLPTDVWELYNAKEDFALTSNLAA
                     KDPERLKKLQALFMSEAQKYNVLPIDDRTIERTNPTLAGRPDVLGDRKTLTLYEGMEG
                     MLENTFMNIKNKSSKVTAKVDVPAGASGAIIAQGGKFGGWSLYMKDGKPAYTYNFLGL
                     SRYTVTAPNALPAGPATVVADFAYDGGGPGKGGKLTLFVNGTAVAEGRVEKTQPNIFS
                     ADETADVGIDNQTPVAVGIGVGADTRFNGRIEEIALEVK"
     gene            complement(4881..5498)
                     /locus_tag="VIV63_03155"
     CDS             complement(4881..5498)
                     /locus_tag="VIV63_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6123624.1"
                     /translation="MNQVPTVFVLDDDVSFLQAVDRLLRAEHFVTRTFGSARQFLEQH
                     DPTTPGCLLTDLIMPDMTGLELQRELLERGCVRPIVFITGQGDMNTAVEGMRAGAVSF
                     LPKPVSRGPLVDCLREGLARDAAIRAARCETQRVASLLASLTPREREVLELVATGLLN
                     KQIAAQLGTAEKTIKVHRGRLMQKMGVKSAAALVQVLNHVEPMAL"
     gene            complement(5627..6898)
                     /locus_tag="VIV63_03160"
     CDS             complement(5627..6898)
                     /locus_tag="VIV63_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY6123625.1"
                     /translation="MSVTTIVLTTLLVTAFVLWIGANLATTEKRLLYRPRRLYTSGDA
                     DFRRALGILLGPPLVPGNHITPLVNGAQIYPAMLNAIRTAQSNITFETFVFRDEIGAT
                     FVDELSAAARRGIHVHMLLDWLGSRTMESNMLAAARAAGCDVQIYHPPSWYHLGRLNN
                     RTHRKIMVIDGKIGFTGGVGMGTEWREGLNGLPPWRETHFKAEGPVVAQMQAVFIDNW
                     IKATGRVLHGAEYFPKLSSTCGDMEAQMFGSSPVGGSESMHLMVLLALTAAKTSIEIQ
                     NAYFVPDRLTVEALCSAARRGVRVRIVVPGRHTDARIGRWAAQGLYGELLAAGIQIYE
                     YQPTMMHCKVLVIDGVWSSVGSANFDDRSFRLNDEANLNVFSEDLAREQVRLIDADIL
                     NSRRMVLNKWRHRKFGRRINERLALLLRSQL"
     gene            7031..7576
                     /locus_tag="VIV63_03165"
     CDS             7031..7576
                     /locus_tag="VIV63_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY6123626.1"
                     /translation="MARSRTRRSAPKDAIALLKADHRQVETWFEQFEKAKADQRKLDL
                     ATRICNALTVHTTIEEEIFYPVFLEATEDKDLHHEAEIEHDAAKKLIAEIEASGPEDD
                     YYDAKVKVLSEMIKHHVKEEEQPGGMFAEARKSDMDLVALGAEMAARKGELEAQGPRA
                     RPAARKSANSILSRIASVATR"
     gene            complement(7573..8751)
                     /locus_tag="VIV63_03170"
     CDS             complement(7573..8751)
                     /locus_tag="VIV63_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY6123627.1"
                     /translation="MSTPTPLADTTLRKAVILLSILAVGALLYFAQDVFIPVAIALFL
                     ALLLNPAVDRLQRWHVRRGLAVAIVLFVVFAAAAVAINAVWTPATDWLARAPQTMSKI
                     DPRLRPLREMFERVDDVAQRAGRLTQGGTAVSGKPAIVTPVDGESTALSITKSFFEWL
                     SIIPFTLFFMLGGPPLLARMGASLAGNEASAKTLRMTEAIREEVGRYFGTVALINLGL
                     GIATGLAMFALGMPNAILWGVLAGVLNFIPYLGPITACFILAGAALVTFPTLSQALAV
                     PGVFVLLHLIEGQLVQPLTVGRRCEVNALVVLLGVWFGFAFWGIAGVLLATPALVALK
                     VAAQHHPSWRVLGDFLSPNSLWNPILLKRRAQVASAREAGATNKMTPAPSPAPTRRSA
                     "
     gene            9015..9686
                     /locus_tag="VIV63_03175"
     CDS             9015..9686
                     /locus_tag="VIV63_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123628.1"
                     /translation="MKGRRSAALLCVLLVHAFVVYSLLNMRRPAARLHADDVFLTEPI
                     TLLLEPEFELEVEPLSAEIPAPSLVRPQAAPPTPARGETAPPAGVSAGVLPPTRIDWP
                     IEGKKSAARVLAAEAEAERIARMFAGPDGTWASLTKRQRSKISKFRFKPGVDGLERDA
                     AGNTIYHLSDGCVLVNFLFFGCAIGKPKVHDDMLENMRLYFDEQRLPRTDEGNGTEPG
                     AQPLP"
     gene            complement(9692..10252)
                     /locus_tag="VIV63_03180"
     CDS             complement(9692..10252)
                     /locus_tag="VIV63_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEY6123629.1"
                     /translation="MTRIVALALTCVLLTPATTFAAKDKAEEQAEVRKAAQSALDALY
                     AVQPAARKAVESAAGYAAFSNFGMKIMVAGSGTGKGMAVNNKTKKQTYMKMAEIQAGL
                     GFGVKKFKLIWVFATQAALDQFVNSGWELGAQATASAKSGDKGASYQGAVAISPDVYL
                     YQLSGNSLAAELTAKGTKYYKDNDLN"
     gene            complement(10268..10663)
                     /locus_tag="VIV63_03185"
     CDS             complement(10268..10663)
                     /locus_tag="VIV63_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123630.1"
                     /translation="MTSVGFRRMLAVMTMAGIACFATAAFAKPSGNWRISFNHQSDND
                     GAIVFRIAPVGGTPIDVEVKVPAKTTENNVADLVSAAIKATVGSEAYRVGVDDGEDVV
                     VKKRGKTPKFELSMVSTSLTGLEIKVGHN"
     gene            complement(10675..11535)
                     /locus_tag="VIV63_03190"
     CDS             complement(10675..11535)
                     /locus_tag="VIV63_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123631.1"
                     /translation="MTINRPATQIKQLTQLLAGSALLAMFASQAAAEDTQLTQQPFQI
                     SLGSFTNASEITIRADGEFGEGTEFDWGDTIGDVDGTTFRLDSYWRINDRHHVRFMYT
                     ENSNRRHKNLDREIEWNGEIIPVDAEVDTEFGFYVVEVAYEYDFSNREDRELVLTAGL
                     HYTAFSAEINGTYATPGGGGGTTTVGSEAKLGAPLPVVGARGMWNLGGNWWLDAQAQW
                     FQIKIDNIDGSILNYRAAVIWQPKQWLGFGVGYDSFGIDVEAEGDRERLRGTLEWTYS
                     GPQAFFNFAF"
     gene            11795..14215
                     /locus_tag="VIV63_03195"
     CDS             11795..14215
                     /locus_tag="VIV63_03195"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6123632.1"
                     /translation="MATESPTDTRSFVSESPAESLWRNSVARNVLAFCCFEVAYYFAY
                     RYGMSFSQDTGSPFWFPDSILLCALLRVPPRWWLPLLVATVPIRFFADVADGISNGML
                     ASTTLNDYAKAVLGATLLRRLMHDPIRFITVRDFGVYCLIAVLALPALSAFAGAAMRG
                     FGPQYWMHWEQWFLGDAMAHLIITPFIFYWVLRPSDQRNLTSAQWLEALILLAGLIAS
                     LALAFQPASTQLGFADSRMYAPVAFLFWAAIRFGMRGATAATLLLTIFAVKAALSGNA
                     TIQSTSMTEGASGLQEYLLLRAAPLYLVAVLLEQAWRIQRSLLESERRFRDMADNAPV
                     MIWITGEDSGCEFVNKGWLDFTGRALSQERGFGWIHGLHADDRQRCMAIYQSSFTARC
                     EFEVDYRLRRMDGVYRWILARGVPRYGEGGKFLGYVGSALDVTDRRRQETALRQSEER
                     YRAVVDSQTELVCRFTPDLTLTFVNEAYCRFLHKQREEVLGSKFSIHMPADTRTQLAL
                     SVVHALSDAGPGEWQCEITFADGSRGWQHWTCQAIHGAGEEPDELQAIGHDVTDRKRA
                     EEAQRRLAHTARLAAVGELTAMVAHEVNQPLCAILSNAEAAETLLSRENPPLDQIREI
                     IKDIRDDDLRADEVIRGIRSLVGRREISIQPIDLNRTVAHVLRLVSGDALFRRVRIQR
                     TLSENLPMVAADQSQIEQVLLNLIVNGMDAMRATPESTRELHLTTRQVDADFVEIAVL
                     DNGCGIPPQRLPELFDSFFTTKAEGMGVGLSIARWIVLAHGGRIWAANRAAGGAEFRF
                     TLRTSLTT"
     gene            14367..15290
                     /locus_tag="VIV63_03200"
     CDS             14367..15290
                     /locus_tag="VIV63_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6123633.1"
                     /translation="MGAPSFAWDIAQLATGHLDVERAVVPLDAAWIGSITVSRTSLHQ
                     VRTDSTCGLVVMPAPESDAIYFEGRRLLAGDSVFVPPDSQMELLTHAVGRIHFVAFPG
                     HSASRFRSRRPRWYCRSDGCAGLGFPEALGALLANAGSPNVDSYSAAALQTRFFNWLR
                     AASIDAESSGTEVPSLPRRRMAVERVRRFIHEHLSESMTLAELCRHAHLQARSLEYGF
                     RDLVGLSPFKYIKMLRLGEVRRRLQMSSPGERSVSEVALDCGFCHLSQFAADYKRVFL
                     ESPSATRRASGRARPARVARYETGPVAFAAR"
     gene            complement(15254..16228)
                     /locus_tag="VIV63_03205"
     CDS             complement(15254..16228)
                     /locus_tag="VIV63_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6123634.1"
                     /translation="MRAVADISRRGLIDTELETLLQPFPGWETEWIHLGPGPASLQGL
                     RVSSGPGTFTTLHTSSAGILRGTTARNSLGLIASLDTTARPRSHAHNIGGDVCLVLGP
                     AATLDVYLPSGGSAMIVGVPAAAPAIHAAAAAGEPARCRTLSSEEGALLSRCMEALEH
                     LRAESASSPAARDIQLRLREHLRTLTPVLMREAGSLAQTERARLQRHLAVIRACTFVD
                     AHLRAPIALMDLCTAAGVSTRALEYGFRDFYGLGPMAYVRNLRLCRVRHDLLDPSRND
                     HSVSGAARRWSFTHMGQFSHDYRALFGEMPSMTLAAHQRAAKATGPVS"
     gene            complement(16311..17873)
                     /locus_tag="VIV63_03210"
     CDS             complement(16311..17873)
                     /locus_tag="VIV63_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase F"
                     /protein_id="HEY6123635.1"
                     /translation="MQQAARRTRLSNYMIPWCAALAFALALSACGSGEQAASPASAAA
                     PDPAEVPTPPAETAATGPSLTGASWLGDLDGMTERRLVRMLVVYNKTNYFFDKAQQRG
                     MTYDMGMELEKWLNRGNKDRARPIRVVFIPTSRDRLLTDLAEGRGDIAAAGLAITPER
                     QRLVNFTAPFADDVSEMLITSADGAVPDTIDALSGRSVYVRRSSSYYTSLEALNARLK
                     ASGKAPVDIELADENLEDEDILEMVNAGLIETTVVDSYLARFWEQVFPNIRLHPELVL
                     RSNSEIAWAVRKDSPKLKAALDPFVAKNEAGTAMGNMILRKYLQTTKWARNATSAADM
                     KRFEELSKYFKKYATQYQFEWLLLVAQGYQESGLDQTTRSPVGAIGVMQVMPTTANDR
                     NVKIPDIHMVEPNIHAGAKYMRFLVDQYFDEPGIDAVNRHLFAFAAYNGGPNRIARLR
                     REAAAQGLDPNKWFNNVELLAAQQIGRETVQYVSNIYKYFVAYKLVVDRAEQRNAAKV
                     RAATASASEKAK"
BASE COUNT         3309 a         5511 c         5778 g         3310 t
ORIGIN      
        1 gacatctgtg cgcgcaaggc cgcggtgttc atcgagcgtg cccgcgccga agacctggtg
       61 agccagcgcg accgccgctt tcagtcggtg ctggaatcct cggccgtgcc cttcatcatc
      121 ctgtcgcccg tgcgggccga caccgggcag atcgtcgatt tccgtttcgt gttcctcaac
      181 accgccgctt cgatcgtcat gcgcgtgcgg gccgaggaat tcgcggggcg caaggtgacc
      241 gacgtgttgc cgcgggcctg ggacgaccgc gcacgattcc acatgtacgt ggatgcgctc
      301 gagaccaacc aggtccgcca gatcgagcgg caatcggaag ccgacggcaa caacaactgg
      361 taccacatcg tggcatcgcc gctcgatggc gacctcgcgg tgtggttcgc ggacatcacg
      421 cagagaaagt tgcaggaacg cgagttgctc gaggccgatc gccgcaagga cgagttcctg
      481 gcgacgctgg cacacgagct gcgcaatccg ctggcgccga tccgccaggc cgcgaccatc
      541 gcgcgaaatg acgccgcgac cgacgcgcag aagcgctggt ccaacaccgt gatcgagcgg
      601 caggtgcaac acatgtcgct gttgctcgac gatctgctcg acgtgtcgcg catcacgcat
      661 ggcacgttgc agttgcgcaa acaacagacc gatctgcaat ccatcgtgag cgcggcggtg
      721 gaaaccgcgc ggccgctcat cgacgagcgg cgtcatcacc tggtcgtcaa tgttccggac
      781 gacctgcagg tgaacgcgga tccgttgcgg ctcgcgcagg tgttgtcgaa tctgctcacc
      841 aatgccgcca agtacaccga tccggaaggc gaaatccgcg tgaccgccga acagcgcgat
      901 gaaagtctca cgatgtgggt cgaagacagc ggcatcggca tcgccaccga agatctgccc
      961 aaggtattcg gcatgttcgc acaggtgcgc tccgcgcagg agcacgccgc gggtggtttg
     1021 ggaatcggcc tggcgttggc gaaaggtatc gtcgaactgc acgggggtcg catcgaagcg
     1081 gcgagcgcgg gagcggggaa gggcagccgg ttcactgtgc ggcttccgaa tcgcatcgta
     1141 tccgtcgctc ctgcttccgc ggtggacgcg ggccgttcaa tgaatggcgt gtcgaacaag
     1201 cgaatcctcc tcgcggacga caatcgtgat gccgccgaaa gtctcgccat cctgctgcgg
     1261 ctcgaagggc acgaagtgga actcgcgcac gacggtcaga ccgcactgca aagtttcgcg
     1321 gcgcgccgcc ccgacgtcgc gctgctcgac atcggcatgc ccaaggccaa tgggtacgaa
     1381 gtggcgcgaa ggattcgcgc cgaacccgat ggcgccggcg tcctgttgat cgcgatcacc
     1441 ggatgggcgc aggacgccga caaggccgag tcgcgcgccg ccggtttcga tcatcacctc
     1501 acgaagccca tcgagccgga aacgctcatg ggcctgctgg atcgtgtcca ggagaagata
     1561 ggtactagcc acggtgggag taaacaccca ctcgtgacag aagcaggtac atgaactacg
     1621 gaccgctgga attcgcgaac tatctccgcc ggatcgacga gagccagaaa gactcggcaa
     1681 ccgtcgaggc ggcgcgttcc gcggccccaa ctactcatcc ggcgaatcgt ctcaccatcg
     1741 tctccggcgg tacgttgctg actcgcgtga cgcgagcgtc gcaggtcgaa gcggtcagcg
     1801 tctacgaggc ggtggcggtt caagcgcccg gtgaatccgg ggccggtgcg gtcgcggtgc
     1861 tagttagggc cacgcttcgg ccggtcgtgc tcgtgttgtc gtcgcaccag accgtgcact
     1921 ggcatctggc gctcgcgccg ggcgcagtgc ttcgtgccgt gctgctgtcg ggatatggag
     1981 aatccacggt gaccggtacg ggcgacgcgc aagtccattc gatcggtggc ttctacgcgt
     2041 tccggcaggg ctcggcggag ttcaggcatc tcgaggccga ggtgctgcgc tgcaccgggc
     2101 ggcccatcga gacctttcgc agcgtatacg ccgggcggtc attcgagatc ggcgacgact
     2161 gagagcggtg cgcggcgccg agaggcggca ccgtagccat gtccatgcgc tttgcgcatg
     2221 gacctttggt cccacgttca acacgtcgtg gacctgagaa tatttctcca aagcaacact
     2281 ggggctccgc tggggccacg cgccggaatc cgtgcgttgt gatgggcgca gcacgatcct
     2341 gattgcgata tcgattcttc gcacatcagc aagccgcggc gggccactaa ctacgatgct
     2401 cgggaacgga gccaacggga gagggtaatc atgaagacac gccgcatgcg cgcgaacacc
     2461 gcaatgggat tggcactcat gtccattgca gccggcgccg ccgacatgga tcggacgatg
     2521 cttcccatcc aggagcccaa gcgcccgacc tattccgaac tcgatgcgcg caacgcgaag
     2581 ccgccggagc gattccaggt caaggcgccg gcgggagcgc cgaacgtcgt catcgtgctc
     2641 atcgacgaca tcggcttcgg tgggccgagc actttcggtg gtccgattcg cacgccgaca
     2701 ttcgattcgc tcgcggccaa cggcctgcga ctcaacaact ttcacaccac cgcgctgtgt
     2761 tcgccgaccc gcgtcgccct gaagtccgga cgcaatcatc attccgcgaa tgccggctcc
     2821 atcatggaga cggcgactgc ctttccgggc aatacgggtc agatccccaa tagcgtcgcg
     2881 ccgctggcgg agatgttgcg gctgaatggc tacagcaccg gtgcgtttgg caagtggcac
     2941 gagaccgccg catgggaaac cagcgtgtcg ggtccgttcg accgttggcc cacccaccag
     3001 ggattcgaca agttctatgg cttcatcggc ggtgagaccg atcagtggta tccgctgatc
     3061 tatgacggcg tgaagaagat cgatcccccg aagatgaagg actaccactt cagcgtcgac
     3121 atgacgaacc agaccatcaa ctgggtcaag gcacagcagt cgatgacgcc tgacaagccg
     3181 ttcttcgtct actacgcgac cggcgccgta catgctccgc atcacgtgcc gaaggaatgg
     3241 gcggacaagt acaagggaca attcgacaag ggttgggacc agatccgcgt cgatacactc
     3301 gcgcgtcaga agaagctggg cctggtgccg gcgaacacga aactcgccga ccgccctgaa
     3361 gatctcaagg cgtggaagac attgccggaa gatcagcggc gcctgttcgc gcgccaggcc
     3421 gaagtgttcg cgggtttcct cgagcacacg gatgaccagg tgggtcgcct gaagaaggcg
     3481 ctcgaggaca tcaacgaatt cgacaacacg ctgttcatct acatcgccgg cgacaacggc
     3541 acgagcgccg aaggcggctt cgtcggcatg tacaacgaga tgacgtactt caacggcatc
     3601 gccgagaaag tggaagacct gattccgctc atcgacaagt ggggcggccc cgacactttc
     3661 ccgcacatgt ccgccggatg ggccgtggct ttcgacacac cgttcaagtg gaccaagcag
     3721 gtggcgtccg atttcggtgg cacccggaac ggggtggtca tcagctggcc gaaaggcatc
     3781 aaggagaagg gtggcctgcg cacgcagttt tcgcacgtca tcgacattgc gcccacggtg
     3841 ctcgaggcgg cgggtttgcc cgaaccgaaa agcgtgaatg gcgtggtgca gataccgctc
     3901 gagggagcga gcctggtcta tgcattcaac gacgccaagg cgcccgagcg ccacacgacg
     3961 cagtacttcg agatgttcgg caatcgcggt atctacagtg acggctggtt cgcgcgcacg
     4021 atccatcgcg cgccgtggca gaccaccaac ctgccgccgc tgcccactga cgtatgggag
     4081 ctgtacaacg cgaaagagga tttcgcgctg accagcaacc tcgccgcgaa ggatcccgaa
     4141 aggctgaaga agctgcaggc gctgttcatg tcggaagcgc agaagtacaa cgtgctgccc
     4201 atcgacgacc gcacgatcga gcgcacgaat cccacgctcg caggccgccc cgacgtgctg
     4261 ggcgatcgca agacgctcac gctgtacgaa ggcatggaag gcatgctcga aaacacgttc
     4321 atgaacatca agaacaagtc gagcaaggtc accgcgaaag tcgacgtgcc ggcgggcgcg
     4381 agcggcgcca tcatcgcgca gggcgggaag ttcggcggct ggtcgctgta catgaaggac
     4441 ggcaagccgg cgtacacgta caacttcctc gggctgtcgc gttacacggt aacggcgccg
     4501 aatgcgctgc ccgcgggccc ggcgaccgtg gtcgcggatt tcgcgtacga cggcggtggc
     4561 ccgggcaagg gcggcaagct gaccttgttc gtgaatggaa cggctgtggc cgaggggcgc
     4621 gtcgagaaaa cgcagcccaa tatcttctcg gccgatgaga ccgcggacgt gggcatcgac
     4681 aatcagactc cggtggcggt cgggatcggc gtcggtgcgg atacccgctt caacggcagg
     4741 atcgaggaga tcgctctcga ggtgaaatga cgcggcgaac gcctcgtcgc gcaactagct
     4801 agccgcggcc ggccgtttga gccggtcgtg ggagtgaacc tgggtgaggc gccgtccagg
     4861 cgctggcgcg cgtataagtc ttagagggcc atcggctcga catgattcaa cacctgcacg
     4921 agcgccgcgg cggacttcac acccatcttc tgcatcaatc ttccgcgatg caccttgatg
     4981 gttttctcgg cggtcccgag ttgcgcggcg atctgcttgt tgagcaaacc cgtcgcgacg
     5041 agttcgagca cttcgcgttc gcgcggtgtc agtgaagcaa gcaggctcgc tacacgttgc
     5101 gtctcgcatc gggccgcgcg gatggcggca tccctcgcga gaccttcgcg caggcagtcg
     5161 accagcggcc cacgcgacac gggtttggga aggaaactga cggcgccggc tcgcatgccc
     5221 tcgaccgcgg tgttcatgtc gccctggccg gtgatgaaca cgatgggccg cacgcaaccg
     5281 cgttccagca gctcacgctg tagttcgagc ccggtcatgt ccggcatgat gaggtcggtc
     5341 agcaggcaac ccggagtagt tgggtcgtgt tgttccagaa actgacgcgc cgaaccaaaa
     5401 gttctcgtga cgaaatgttc tgcgcgcagc aaccggtcga cggcctgcag aaacgaaacg
     5461 tcatcgtcga gaacgaagac cgtggggacc tggttcattg ctcactcccg atttgcggag
     5521 gtgggccccc gtctttgggt tgcgattatg agctagtcat cagccgatac ctactttcgt
     5581 ataggtacgg cgaccgcaaa tgattggttt cgcgcgtaaa tgattgctag agctgcgacc
     5641 gcagcaagag tgccagccgt tcgttgatcc ggcggccaaa tttccggtgc cgccacttgt
     5701 tgagcaccat gcgccgggaa ttcaggatgt cggcgtcgat caggcggacc tgttcccgtg
     5761 ccaggtcttc gctgaacaca ttgaggttgg cttcgtcgtt gagccggaac gagcggtcat
     5821 cgaaattcgc ggagcccacc gagctccaga cgccgtcgat caccaggacc ttacagtgca
     5881 tcatcgtcgg ctggtattcg tagatctgga tgccggcggc taacaactcg ccatacagcc
     5941 cttgcgcggc ccagcggccg atgcgcgcat cggtgtgacg gcccggcacg acgatgcgca
     6001 cgcgcacgcc acgcctcgca gcggaacata gcgcttccac cgtgagccgg tcggggacga
     6061 aataggcgtt ctggatttcg atcgaagtct tcgcggcggt gagtgccagc agcaccatga
     6121 gatgcatgct ctcggagcca ccgaccggcg agctgccgaa catctgtgct tccatgtcgc
     6181 cgcacgtgga ggagagcttg ggaaaatatt cggcgccgtg cagcacgcgc ccggtggctt
     6241 tgatccagtt gtcgatgaac accgcctgca tctgcgcgac cacgggtcct tcggccttga
     6301 aatgcgtctc gcgccacggt ggcaggccgt tgagtccttc gcgccattcc gttcccatgc
     6361 cgacgccgcc ggtgaaaccg atcttgccat cgatgaccat gatcttgcgg tgtgtgcggt
     6421 tgttgagccg gccgaggtga taccaggaag gcggatggta gatctgcacg tcgcaaccgg
     6481 ccgcacgcgc cgcggccagc atattggatt ccatggttcg cgagccgagc cagtcgagca
     6541 gcatgtggac gtggatgcca cggcgcgccg cggccgacag ctcatcgacg aatgtcgcgc
     6601 cgatctcgtc gcggaacacg aaggtctcga acgtgatgtt gctctgcgcc gtgcggatgg
     6661 cgttgagcat ggcgggatag atctgtgcgc cgttgaccaa tggcgtgatg tggttgcctg
     6721 gaacgagcgg cggaccgaga aggatgccga gcgcgcggcg gaagtcggca tcgccactgg
     6781 tgtagagccg gcgagggcga taaagcagtc gcttttcggt ggtggcgagg ttcgcgccga
     6841 tccacagcac gaacgcggtg acgaggaggg ttgtgaggac gatcgtggtg acggacacgt
     6901 gaattattta cagttgagaa ggagtcgcaa gtgacgtggg atgcacctta cacaggtctg
     6961 catgatcgtt tgctatcgac gctgttcgca agcgcctagt gtgcctcaca acacacgagg
     7021 agtagggcac atggccagat cgaggacgcg ccgctcggcg cctaaagacg cgattgcatt
     7081 gttgaaggcc gatcaccgcc aggtggaaac ctggttcgaa cagtttgaaa aggcgaaagc
     7141 cgatcagcga aagctcgatc tggcgacgcg catctgcaat gcgctcacgg tgcataccac
     7201 catcgaggaa gagattttct atccggtttt tctcgaagcc accgaagaca aggatcttca
     7261 ccacgaggcg gagatcgaac acgatgccgc gaagaaactg atcgccgaga tcgaggcgtc
     7321 gggacccgag gacgactact acgacgcgaa ggtcaaagtg ttgtccgaga tgatcaaaca
     7381 tcatgtgaag gaagaagagc agccgggcgg catgtttgcc gaggcgcgga agtcggacat
     7441 ggacctcgtc gcgctcggcg ccgaaatggc ggctcgaaag ggtgagctcg aagcgcaggg
     7501 tccgcgggcc cgtccggccg cgcgcaaatc cgcgaattcc atactttcgc gcatcgcctc
     7561 ggtggccact cgctaggctg aacggcgcgt tggggcggga ctcggggccg gcgtcatctt
     7621 gttggtggcg ccggcttctc tcgccgatgc gacttgcgcg cgccgcttga gcaggatcgg
     7681 attccacagc gaattcggcg acaggaaatc acccagcact cgccaggatg ggtgatgttg
     7741 cgcggctacc ttgagcgcaa ccagcgcagg cgtcgctaac aggacgccgg caatccccca
     7801 gaatgcgaag ccgaaccaca cgccgagcaa caccaccagc gcgttcacct cgcagcgtct
     7861 gcccacggtc aaaggctgca cgagttggcc ttcgatgaga tgcagcagga caaacactcc
     7921 cggcacggcc agtgcctggc tgagcgtcgg gaaagtcacg agcgccgcgc ccgcgagaat
     7981 gaagcaggcg gtgatgggtc cgagatacgg aatgaagttc agcacgccgg cgagcacgcc
     8041 ccacaggatc gcgttgggca tgccgagcgc aaacatggca aggccggtgg cgatacccaa
     8101 accgagattg atcagcgcga cggtaccgaa atagcgcccg acctcttcgc gtattgcttc
     8161 agtcatgcgc agggttttcg cgctcgcctc gttcccggcg agggatgctc ccatgcgtgc
     8221 gagaagcggt gggccgccga gcatgaagaa caatgtgaac gggatgatgg acagccattc
     8281 gaagaaggat ttggtgatgg acagcgcggt gctctcgcca tccaccggcg tcacgatggc
     8341 aggtttgccg ctgacggcag tgccaccctg ggtgagccgg ccggcgcgct gggccacatc
     8401 gtcgacacgc tcgaacattt cgcgcagcgg tcgcagccgc ggatcgatct tgctcatggt
     8461 ctgcggcgcg cgcgcgagcc aatccgtggc gggtgtccag actgcgttga tcgccacggc
     8521 ggcggcggcg aacaccacga acaggacgat ggccacggcg aggccgcgac gcacgtgcca
     8581 gcgctgcagc ctatctactg ccggattcag caggagcgcc aggaacagcg cgatcgccac
     8641 tggaatgaag acgtcctgcg cgaagtagag cagagcgccc accgcgagta tcgagaggag
     8701 aatcactgct tttcgcagcg tggtatcggc aagaggggtg ggggtgctca ccgcgccaat
     8761 gtcgggcata cgctgacacg cgggcaggcg agaaacctcc gcacactggg acggaatccg
     8821 cccggctcgt tgtaggccgg gtcccacacc ctcaagcccc gtatgatcgc tatatataag
     8881 agtggggtcg gttggcggtg tcgacgcgag atgcaggagt ccgggcaaac gtcgcaggcg
     8941 tcgaggaatt tatcgacgcc ggcaatcctg cgtcagcggg aaccggacat tcgccgcgga
     9001 gtcgtattcc atcaatgaaa gggcggcgtt cagcggcatt gctctgtgtg ctgctggtgc
     9061 atgcgttcgt tgtctattcg ctgctgaaca tgaggcgacc ggcagcacgc cttcacgctg
     9121 atgacgtctt tctcaccgaa cccatcacct tgctgctcga gcccgaattc gagctcgagg
     9181 tcgagcctct ttccgcggaa attccggcgc cgagtctggt gaggccgcag gcagcgccac
     9241 cgacacccgc tcgaggcgaa acggcgccac ccgcaggcgt ttccgccgga gtcctgcctc
     9301 ccacgcgcat cgactggcca atcgaaggta agaagtccgc ggcgagggtc ctggcggcgg
     9361 aggccgaagc ggagcgcatc gcaagaatgt ttgcaggtcc cgatgggact tgggcttcgc
     9421 tgacgaagcg gcagcgctcg aagatcagca agttccgctt caaaccgggc gtggacggac
     9481 tcgagcgcga tgcggcggga aacactatct accacctgag tgatggctgc gtgctcgtga
     9541 acttcctgtt cttcggttgc gcgatcggca agcccaaggt tcacgacgac atgctggaga
     9601 acatgcgcct gtatttcgac gaacagcgcc tgccgcgaac cgacgaaggc aatggaaccg
     9661 agcccggggc gcaaccgctg ccctgagaac ttcagttcag atcattgtcc ttgtaatact
     9721 tcgtgccctt cgcggtcagc tccgccgcga ggctgttgcc actcaactgg tacagataga
     9781 catccggcga gatggcgaca gctccctgat aggacgcgcc cttgtcaccg gatttcgctg
     9841 aagccgtcgc ctgtgctccc aactcccagc cggagttgac gaactggtcg agcgccgcct
     9901 gcgtcgcgaa aacccagatg agcttgaact tcttgacgcc gaatcccagg cccgcctgga
     9961 tctcggccat cttcatgtac gtctgtttct tggtcttgtt gttgaccgcc atgcctttgc
    10021 cggtgccgct gcccgcgacc atgatcttca tgccgaagtt gctgaacgcg gcatagccgg
    10081 cggccgactc cacggctttg cgtgccgccg gttgaacggc gtagagcgca tcgagcgcgg
    10141 actgcgccgc cttgcgaact tcggcctgtt cttcggcctt gtccttggcc gcgaacgtgg
    10201 ttgccggcgt caggagcacg catgttagtg ccaacgcgac gatacgagtc atcatgacct
    10261 tctcccgcta gttgtgaccg accttgatct ccaggccagt cagagatgtg ctcaccatgg
    10321 aaagttcgaa tttcggtgtc ttgccgcgct tcttcaccac cacgtcttcg ccatcgtcga
    10381 cacccacgcg ataggcctcg gaacccaccg tcgccttgat ggcggcgctc acgaggtcgg
    10441 cgacattgtt ctcggtggtt ttcgccggca ccttgacttc gacatcgatg ggcgtgccgc
    10501 ccaccggcgc aatgcgaaac acgatggcgc cgtcgttgtc ggactggtgg ttgaagctga
    10561 tgcgccagtt acccgagggt ttggcaaacg cggcagtggc aaagcaggcg attcctgcca
    10621 tcgtcatcac cgcgagcatt ctccggaagc ccaccgacgt catggctctc tccgtcaaaa
    10681 cgcgaagttg aagaacgcct gcggacccga gtacgtccac tcgagggtgc ccctcagccg
    10741 ttcacgatca ccttccgcct cgacatcgat gccgaaggag tcgtagccaa caccgaagcc
    10801 taaccactgc ttcggctgcc agatgaccgc ggcgcgatag ttgaggatgc tgccgtcgat
    10861 gttgtcgatc ttgatctgaa accactgcgc ctgggcgtcg agccaccagt tgccgccgag
    10921 attccacatg cctcgcgcgc cgactacggg aagcggcgcg ccgagcttcg cctcgctgcc
    10981 gaccgtggtg gtgccgccac cgccaccggg cgttgcatag gtgccgttga tctcggcgga
    11041 aaaggcggtg tagtgcaaac cggcggtcag tacgagctca cgatcctcgc gattcgagaa
    11101 gtcgtattca tacgcgacct ccacgacgta aaagccgaat tcggtgtcga cctcggcgtc
    11161 gaccgggatg atttcaccgt tccattcgat ttcgcgatcg agattcttgt gccggcgatt
    11221 ggaattctcc gtgtacatga agcgcacatg gtgccggtcg ttgatgcgcc agtagctgtc
    11281 gagcctgaat gtggtgccgt ccacatcgcc aatcgtatcg ccccagtcga actcggtgcc
    11341 ttctccgaat tcgccatcgg cgcggatcgt gatctccgac gcgttggtga aagagccgag
    11401 cgaaatctgg aacggctgtt gcgtcagctg tgtgtcttca gccgccgcct ggctcgcaaa
    11461 catggcgaga agcgcgctcc cggcgagaag ctgcgtgagt tgcttgatct gggtcgccgg
    11521 gcggttgatg gtcatgttcg attcctcgga atcttcggca ctgggggatt tcttcgtggt
    11581 tgatagtgag ccggcgccac tgggtgtcta tcgtgaaatc gcacgctgca gagcgtcatt
    11641 gcacgccgga atcctcgccg tggagctctc gtcccgcctg ttgtacgagc cggcgtcgcc
    11701 tccgtgaagt gggaccttgg tctgatggtg cgtcgcggag gggtgtttgg cggttgtcgt
    11761 tgttgcgcaa aggtcggacg ggcaagatgc gcccatggcc acggagtcgc cgaccgatac
    11821 ccgcagtttt gtcagcgaat cgcccgccga gtcgctgtgg cggaattcag tcgcccgtaa
    11881 cgtgctcgcc ttctgttgtt ttgaagtcgc gtactacttt gcctaccgct acgggatgtc
    11941 cttcagccag gacaccggct cgccgttctg gtttcccgac tccatattgc tctgcgccct
    12001 gttgcgagtc ccacctcgct ggtggttgcc gttgctcgtg gcgacggtgc ctatccgctt
    12061 cttcgcagat gtggccgatg gcatctcgaa cggcatgctc gccagtacca cgctgaacga
    12121 ctacgccaag gctgtgttag gcgccacgct gctgcggcgt ctcatgcacg acccgatccg
    12181 cttcatcacc gtgcgcgatt tcggtgttta ttgcctgatc gccgtgttgg cgctgccggc
    12241 attgagcgcg ttcgcaggcg ccgccatgcg gggcttcggc ccgcaatact ggatgcattg
    12301 ggaacaatgg ttcctgggcg acgcgatggc gcatctgatc atcacgccct tcatcttcta
    12361 ctgggtgctg cggccttcgg atcagcgcaa cctcacctcc gcgcagtggc tggaagccct
    12421 gatcctgctc gccggactga tcgcgagcct ggcgctggcg ttccaacccg cttcgaccca
    12481 gctgggcttc gccgattcgc gaatgtacgc gccggtggcg ttcctcttct gggcggcgat
    12541 tcgctttgga atgcggggcg cgacggcagc aacccttctg ctcacgatat tcgcggtcaa
    12601 ggcggcgctg tcgggcaatg ccacgatcca gagcacgtcg atgacggagg gcgcatcggg
    12661 tctgcaggag tacctgctgt tgcgcgcggc gcccctgtac ctggtggcgg tgctgctgga
    12721 acaggcctgg cgcatccagc gctctctgct cgagagcgag cggcgcttcc gtgacatggc
    12781 cgacaacgcg ccggtgatga tctggatcac cggcgaggac tcgggctgtg aattcgtcaa
    12841 caagggctgg ctcgatttca ccgggcgtgc gctgtcgcag gagcgcggat tcggctggat
    12901 tcatggcctg catgccgacg accggcaacg ctgcatggct atctaccagt ccagctttac
    12961 ggctcgatgc gaattcgaag tcgactatcg gctgcgccgc atggacggcg tgtatcgatg
    13021 gatcctcgcc cgcggcgtgc cgcggtatgg cgagggcgga aaattcctgg gctacgtcgg
    13081 ctccgcgctc gacgtcaccg accgccgccg gcaggaaacg gcgttgcgcc agagcgaaga
    13141 acgttatcgc gcggtcgtcg acagccagac ggagctggtg tgccgcttca cgccggacct
    13201 gacgctgacc ttcgtcaacg aggcctattg ccgctttctc cacaaacaac gcgaggaagt
    13261 gctgggctcg aaattcagca tccatatgcc tgccgatacg cgcacgcaac tcgcgctgag
    13321 tgtcgtgcac gcattgtcgg atgccgggcc cggcgaatgg cagtgcgaaa tcactttcgc
    13381 ggatggcagt cgcggctggc aacactggac ctgccaggcc atccacggcg ccggcgagga
    13441 gccggacgaa ttgcaggcga tcggccacga cgtcacggat cgcaagcgcg ccgaagaagc
    13501 gcagcgccgg ctcgcgcata cggctcgcct ggcggcggtg ggcgagctca cggccatggt
    13561 ggcgcatgaa gtcaatcaac ctctgtgcgc gattctcagc aacgccgagg ctgccgagac
    13621 gctgctgtcg cgcgagaatc ctccgctcga tcagattcgc gagatcatca aggacattcg
    13681 cgacgacgat ctgcgggccg acgaagtcat tcgtggaatt cgttcgctgg tcgggcgacg
    13741 cgagatatcg attcagccca tcgacctgaa ccgcaccgtg gcgcacgtgc tgcggctcgt
    13801 gtccggcgac gcgctgttcc ggcgtgtgcg cattcagcgc acgctgtccg aaaacctccc
    13861 gatggtcgcc gccgaccagt ctcagatcga acaagtgctg ctgaacctca tcgtcaatgg
    13921 tatggacgcg atgcgggcga cgcctgaatc gacacgcgag ttgcatctga cgacgcggca
    13981 ggtggatgcg gacttcgtcg aaatcgcggt cctcgacaac ggttgcggca tacctccgca
    14041 gcgattaccc gagttgttcg attctttctt caccaccaag gccgagggca tgggcgtcgg
    14101 cctgtcgata gcccgctgga tcgtgcttgc gcacggcggg cgcatctggg cggcgaatcg
    14161 cgccgcaggc ggcgccgaat tccgcttcac gctgcgcact tccctaacta cataagggga
    14221 aatggccgta ggaccaaggt ccgatgttcg ccgtggcgca cggtgtctta aaaagggcac
    14281 acggtcgcgc aacagcgcga cgtcctcggg agcggacggc ggcgatgctg gctatcgaac
    14341 acaaccctgc ggggtttggc gcagcaatgg gtgccccaag cttcgcctgg gacattgcgc
    14401 aactcgccac cggccacctc gacgtcgagc gggcagtggt gcccctggat gcggcctgga
    14461 ttggcagtat caccgtgagc cgcacgtcgc tgcaccaggt gcgaaccgat tcgacctgcg
    14521 ggttggtcgt gatgcccgcg ccggaatccg acgccatcta tttcgaagga cgccgcctgc
    14581 tggcgggcga ttccgtgttc gttccgcccg acagccagat ggagttgctc actcacgccg
    14641 tgggtcgtat ccatttcgtc gcctttccgg gccactctgc atcccgcttc cgatcgcgcc
    14701 ggcctcggtg gtattgccgc tctgacggtt gtgcgggcct gggatttccc gaagcactgg
    14761 gcgcgttgct cgcgaacgcg ggttcgccaa acgtggattc atattcagcg gcggcccttc
    14821 agacccgctt cttcaactgg ctgcgtgcgg cgtccataga cgccgagtcg tccggcaccg
    14881 aagttccatc gctgccgcgc cgccggatgg cggtggagcg tgttcgccgc ttcatccacg
    14941 aacacctgtc cgaatccatg acgctcgccg aactctgtcg ccatgcgcac ctgcaggccc
    15001 gctcgctcga gtatggattc cgcgatctcg tcggtttgtc accgttcaaa tacatcaaga
    15061 tgcttcggct cggcgaagtt cgccgccgtc tgcagatgag cagtcccggg gaacgcagcg
    15121 tctccgaagt ggcgctcgac tgcggcttct gccacttgag ccagttcgcg gccgactaca
    15181 agcgcgtatt cctggagagt ccttcggcca cgcgccgggc gtcaggccgc gcaaggcccg
    15241 cgcgagtcgc gcgttacgaa accgggccgg tcgcctttgc tgcccgctga tgcgcggcca
    15301 gcgtcatcga aggcatttct ccaaacaacg ctcgatagtc gtggctgaac tgacccatgt
    15361 gggtaaaact ccagcgacgc gcggctccgc tgaccgaatg gtcgttgcgc gaaggatcca
    15421 gcaggtcgtg acgcacgcgg cataggcgca gattgcgtac gtaagccatg ggcccaaggc
    15481 cgtagaaatc ccggaaacca tattcgagtg cgcgtgtgct cacgccggcg gcggtgcaca
    15541 ggtccatgag cgcgatcggc gcacggagat gggcgtcgac gaaggtgcag gcccggatca
    15601 cggcaaggtg gcgctgcaga cgcgcccgtt cggtctgggc aagcgagcct gcctcccgca
    15661 tcaacaccgg ggtcaatgtg cgcaggtgct ctcgcagccg aagctggata tcccgcgctg
    15721 ccggggacga ggcggattcg gctcgcagat gttccagcgc ctccatgcac cgcgacagca
    15781 gggcgccttc ttccgagctc agcgtgcggc atcgggcagg ttcgccggcg gcggcggccg
    15841 cgtgaatggc cggcgccgcg gcgggaacgc ccacgatcat ggcgctgccg ccggacggta
    15901 gatagacatc cagcgtcgcc gccgggccca gtaccaggca gacgtcaccg ccgatgttgt
    15961 gtgcgtggct gcgaggccgg gcagtggtat ccaggctcgc aatcagcccg agactgttgc
    16021 gcgccgtggt gccgcgcagg atgccggcgg aggaggtatg gagagtggtg aaagtcccgg
    16081 gaccggacga cacccgcaat ccctggagac tcgccggtcc aggacccaga tgtatccact
    16141 ccgtttccca gccggggaag ggctgcagga gcgtttccag ttcggtgtcg atgaggcctc
    16201 gtcgtgaaat gtctgccacc gcacgcatcc ttggcgactt ccttcgggga agtgctagtg
    16261 gaatcggtgg cggggcgcgt gactatcaag aaaacgtcac ttgcaccgtt ttatttcgct
    16321 ttttcggacg ccgaggccgt tgccgcccgg acttttgccg cgttgcgctg ctccgcgcgg
    16381 tcgaccacca gcttgtacgc gacgaagtac ttgtaaatgt tgctcacgta ttgaacggtt
    16441 tcccggccta tctgctgggc cgccagtagt tcgacgttgt tgaaccactt gttcgggtcc
    16501 agcccctgcg cggccgcctc acggcgcaac ctcgcgatcc ggttggggcc gccgttgtag
    16561 gccgcgaacg cgaacaagtg acgattgacc gcatcgatgc ccggttcgtc gaaatactgg
    16621 tcgaccagga agcgcatgta tttggcgccc gcatggatgt tcggctcgac catgtggatg
    16681 tcggggatct tcacgttgcg gtcattggcc gtggtgggca tgacctgcat gacgccgatc
    16741 gcccccaccg ggctgcgcgt ggtctgatcg agtccggatt cctggtatcc ctgcgccacc
    16801 agcagaagcc attcgaactg gtactgcgtg gcgtatttct tgaagtactt cgacagttct
    16861 tcgaagcgct tcatgtcggc cgccgacgtg gcattacgcg cccacttggt ggtctgcagg
    16921 tacttgcgca ggatcatgtt gcccatggcc gtaccggctt cgttcttcgc gacgaacgga
    16981 tcgagggctg ccttgagctt cggagaatcc ttgcgcacgg cccatgcgat ctcgctgttg
    17041 ctgcgcagga ccagttccgg atgcagacgg atgttcggaa acacctgttc ccagaaccgc
    17101 gcgaggtaac tgtcgaccac ggtggtctcg atgagcccgg cattcaccat ttcgagaatg
    17161 tcttcgtctt cgagattctc gtccgccagt tcgatgtcga cgggtgcctt gccggacgcc
    17221 ttgagcctgg cattcagcgc ttcgagactc gtgtagtaac tgctcgaacg ccgtacgtag
    17281 acgctgcggc cggaaagcgc gtcgatggta tcgggaacgg cgccgtcggc ggaggtgatc
    17341 agcatttcgc tgacgtcatc ggcgaaggga gcggtgaaat tcaccaggcg ctgtcgctcg
    17401 ggcgtgatgg cgaggcccgc ggctgcgatg tcaccgcgtc cttcggcaag gtcggtcagc
    17461 aggcgatcac ggctcgtggg aatgaatacg acgcgtatgg gccgcgcacg gtccttgttg
    17521 cctcggttga gccatttttc gagctccata cccatgtcgt aggtcatgcc gcgctgttgc
    17581 gccttgtcga agaaatagtt ggtcttgttg tagacgacca gcatgcgaac gagacggcgt
    17641 tcggtcatgc catcgagatc gccgagccac gatgcgccgg taagagacgg gccggtcgcg
    17701 gcggtctctg ccgggggcgt gggcacttcg gccgggtcgg gcgccgcggc ggatgcgggt
    17761 gacgcggcct gttccccgga gccgcatgcg gacaacgcca gcgcgaacgc cagcgccgcg
    17821 caccacggga tcatgtagtt agataatcgt gtgcgacggg ctgcttgctg catgggcaca
    17881 tgctatgagt cggctcaggc ggcagcaa
//
LOCUS       DATDEU010000130         4351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1336627, whole genome shotgun sequence.
ACCESSION   DATDEU010000130 DATDEU010000000
VERSION     DATDEU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336627"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VIV63_03215"
     CDS             complement(<1..414)
                     /locus_tag="VIV63_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123636.1"
                     /translation="MSTRALVTLFAVPLLVLAGCSREKGDWRSAQAADSPESYEQFIA
                     EHPDSSLVATARDRLQQLAEEKDWRAAAVTDSLEAYQQFLAQYPAGKWAKEAQVRIDN
                     FGNTAGATTAGAAASADAAATASPAGEGAALPDAAL"
     gene            475..873
                     /locus_tag="VIV63_03220"
     CDS             475..873
                     /locus_tag="VIV63_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6123637.1"
                     /translation="MSEEPKRRLVDRLGMSPTVIAEGTTIVGDIETRGPLMVSGHVRG
                     DGKIGGTLSVGKSAHWEGDISARQAVLAGKVTGRIVVEDKLEISASAVISGEIVAKIL
                     AIANGARIEGEVTVTSGKPILKFEEKRAAG"
     gene            complement(870..3407)
                     /locus_tag="VIV63_03225"
     CDS             complement(870..3407)
                     /locus_tag="VIV63_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY6123638.1"
                     /translation="MVFTASRLRAGLLIQALAVLLVSCGGGSDDDPPPPPPPAADTTA
                     PSIPGGVTAVAQSATQILVSWTASTDASGISGYRVYRNGGATALATVQTTNYTDSGLS
                     AATAYTYTVSAVDSAATPNESAQSGSVSATTTGGTPPAGGLDARPSNTSCLAGDPPAS
                     TVSLAVQRVFGGLPNFTQPIAMLQEPGNSARWYVVQKTGSVRVFDNTPSASTTREFIN
                     IASRLNSNPSSSNDERGLLGMAFHPNYPADPRVYLFYTGTDTTLGLVDRVSQFRLASN
                     GTTLDPTTELELFNVDDPESNHNGGHIAFGPDGFLYIGIGDGGSGGDPHGTIGNGQNR
                     QTMLGKVLRIDIAAASSTNTYTIPSNNPYVGNARCNATGTGTANCPEIFAYGFRNPWR
                     WSFDRGSGQLWLNDVGQGALEEVDLVVRGGNYGWRCLEGTSNTGDACGPNPSPIAPVA
                     QYGRTLGFSTTGGVVYRGSAIPALHGRYVFGDFGSGNLWHIARDTAPTLNLTADMALS
                     TGLQIASFGEDTNGEVYIVHLGGTLHRLAQGAGGGRQIPAQLSQTGCVNATTPTQPAS
                     GLIPYAPNAAFYSDGAVKARWLALPDGQRIVVNAENDFEFPNGSVLVKNFSIGTQLVE
                     TRLFMRHSNGAWAGYTYEWDAAGADATRVVGGKTVQVAGQTWQFPSEAQCLQCHSAAA
                     GRTLGLEIGQLNGDFGYPARTANQLTTLNAIDTLTPALTQTPAQLPLIPDPFGSAALG
                     SRARAYLHTNCAHCHRPGGPATGDLDFRYTTAIAATNACDVTPTFGNLGLTNARIIAP
                     GTAASSVVVARVNRVGTDAMPPLARHRIDTAGVQLLTDWVNGLANCN"
     gene            complement(3482..>4351)
                     /locus_tag="VIV63_03230"
     CDS             complement(3482..>4351)
                     /locus_tag="VIV63_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6123639.1"
                     /translation="ELLLECGDGVVLQAFHASPAKCGREPGKTLAVLLHGWEGSADST
                     YVLSVAQTLFERDFEVVRLNLRDHGTTHHLNRDLFHSCLLPEVVGAVLSLAQRFANLP
                     IVLVGFSLGGNFMLRVAADREARDLPLQRVLAVSPVLDPAVTMTTLERGSPLYHGYFV
                     RKWTRSLAKKQLAWPDHYNFDDLLRIRSLREMTRQLVERHTEYPSMDDYLAGYAITGD
                     RLTTLAAPATVLTSLDDPIIEARDLARLARSPRLDIVTTAHGGHCGFLENLGGSNWVD
                     TLLVKLLSHNPDR"
BASE COUNT          754 a         1330 c         1520 g          747 t
ORIGIN      
        1 cagcgctgcg tcgggcagcg cggcaccctc gcccgcggga ctggccgttg ccgccgcgtc
       61 ggctgacgct gcggcgccag cagtcgtggc gcccgcggtg ttgccgaagt tgtcgatgcg
      121 cacctgcgct tccttggccc acttgccggc cggatattgc gcgaggaatt gctgataggc
      181 ctcgagtgaa tcggtgactg cggcggcgcg ccaatccttt tcctcggcga gttgctgcag
      241 gcggtcgcgc gcggtcgcga ccagcgaact gtcgggatgc tccgcgatga attgctcgta
      301 cgactcggga gaatccgctg cctgcgccga gcgccagtcg cccttctcgc gactgcagcc
      361 ggcgagtaca agcagcggga ctgcgaacag cgtcacgaga gcacgagtgg acatggttat
      421 gatcccctgg aacgaacggt ttattgtaaa cgcagccgcc gaccggagat gcacatgagc
      481 gaagaaccca agcgcagact cgtcgaccga ttgggcatgt cccccaccgt cattgccgaa
      541 ggcaccacga tcgtgggtga catcgagacg cgtggaccgc tcatggtgtc aggtcacgtg
      601 cggggcgatg gcaagatcgg cggcacgttg agcgtgggca agagcgctca ctgggaaggt
      661 gacatttccg cgcgccaggc cgtgttggcc ggcaaggtca ccggcaggat cgtggtcgaa
      721 gacaagctcg agatcagcgc cagcgccgtc atctccgggg agatcgtcgc gaagattctc
      781 gccattgcca atggcgcgcg aatcgaaggc gaagtgaccg tgacgagcgg aaagccgatc
      841 ctcaaattcg aagagaagcg cgccgccggc taattgcagt tagccaggcc gttcacccag
      901 tcggtcaaca actgcactcc cgccgtatcg atccgatgcc gtgccaatgg cggcatggcg
      961 tccgttccca cgcgattgac ccgcgccacc accactgagc tcgcagccgt tcctggcgcg
     1021 atgatgcgcg cgttggtgag acccaggttc ccgaaggtcg gcgtgacgtc acaggcattg
     1081 gtggcggcga tcgccgtggt gtaccggaag tcgagatctc cggtcgccgg cccacccggg
     1141 cgatggcagt gcgcgcaatt ggtgtgtaga taggcgcggg cgcgagatcc caaagccgcg
     1201 ctgccgaaag gatcggggat cagcggaagt tgcgccgggg tctgggtcag cgccggcgtg
     1261 agcgtatcga tggcgttgag cgtggtgagc tgattggccg tgcgggcggg ataaccgaaa
     1321 tcgccgttga gctgacctat ctcgagtccc aacgtgcggc cagccgcggc cgaatggcat
     1381 tgcaggcact gggcctcgct cgggaattgc caggtttgac cggcgacctg cacggtcttg
     1441 ccaccgacga cgcgtgtggc gtcggcgccc gcggcatccc attcataggt gtaaccggcc
     1501 catgcaccgt tgctgtggcg catgaacagc cgcgtttcga cgagctgcgt gccgatgctg
     1561 aagttcttca ccagcacgct gccgttcggg aattcgaagt cgttttccgc gttgacgacg
     1621 atacgctgcc catcgggcag cgcgagccag cgcgccttca ccgctccgtc ggagtagaac
     1681 gcggcattgg gcgcgtacgg aatgagtccg ctggccggtt gagtcggcgt ggtggcgttc
     1741 acgcagcctg tctgggagag ctgtgcgggg atctgccggc caccgcccgc gccctgcgcc
     1801 aggcgatgca gcgtgccacc gagatggacg atgtacacct cgccgttggt gtcttcaccg
     1861 aaagacgcaa tctgaaggcc ggtcgaaagc gccatgtcgg cggtgagatt cagcgtcggc
     1921 gccgtgtcgc gcgcgatgtg ccagagattg ccgctgccga aatcgccgaa tacgtaacgg
     1981 ccgtgcagcg cgggaatggc gctgccgcga taaacgacgc cgcccgtcgt ggagaacccg
     2041 agagtgcggc cgtactgcgc cacgggcgca atcggcgatg gattgggacc gcaggcgtcg
     2101 ccggtgttgc tggtgccttc gaggcatcgc cagccgtagt tgccgccgcg aaccacgaga
     2161 tccacttctt cgagtgcgcc ctggccgaca tcgttgagcc agagctggcc gctgccgcga
     2221 tcgaagctcc agcgccaggg attgcggaag ccgtacgcga agatctcggg gcagttcgcc
     2281 gtgcccgtgc cggtggcgtt gcagcgcgcg ttgccgacgt agggattgtt ggaggggatg
     2341 gtgtacgtgt tcgtggagct tgcggccgcg atgtcgatgc gcagcacctt gcctaacatc
     2401 gtctgacggt tctggccgtt gccgatggtg ccgtgcggat cgccgccgct gccaccgtca
     2461 ccaatgccga tgtagagaaa accgtcgggg ccgaacgcga tgtggccgcc gttgtgattg
     2521 ctctccgggt catcgacgtt gaacagctcg agttcggtgg tgggatcgag cgtggtgccg
     2581 ttgctcgcca gccggaactg cgacacgcga tcgaccaggc cgagcgtcgt gtccgtgccc
     2641 gtatagaaga ggtacacgcg agggtcggcc ggatagttcg ggtggaacgc catgccgagc
     2701 agtccgcgct cgtcgttgga gctggacgga ttggagttga ggcgcgacgc gatgttgatg
     2761 aactcgcgcg tcgtcgacgc gctcggggtg ttgtcgaaaa cgcgcaccga tcccgtcttc
     2821 tgcacgacgt accagcgcgc tgaattgccc ggctcctgga gcatggcgat gggctgcgtg
     2881 aaattcggca ggccgccaaa cacgcgctgc acggccagcg acaccgtgct ggccggcgga
     2941 tcgccggcga ggcaggacgt attcgaagga cgggcgtcga ggccgccggc cggcggcgtt
     3001 ccaccggtgg tggtggcgct cacggaaccg gattgcgccg actcgttcgg cgtggccgcc
     3061 gaatcgacgg cgctcacggt ataggtgtag gcggtcgcgg cgctgagccc gctgtcggtg
     3121 tagttagtgg tctggacggt ggcgagcgcg gtggcaccac cgttgcggta gacgcgatag
     3181 ccgctgatgc cgctcgcatc cgtcgacgcg gtccagctca cgaggatctg cgtcgcggac
     3241 tgcgcgacgg cggtcacgcc gcccggaata ctgggcgccg tggtatcggc ggccggcggt
     3301 ggtggtggcg ggggatcatc gtcggagccg cccccacaac tcacgaggag gaccgcgagc
     3361 gcctgaatca gcagacccgc cctgagccgc gatgcggtaa agaccatctc caaagacctc
     3421 tgagcttcca ggacttgcgt ccgtgggttg ttagcagctc agcacactcg gctgaggctg
     3481 cctatctgtc cgggttatgc gacagcaatt tgaccaacag ggtatccacc cagttgctgc
     3541 cgccgaggtt ctcgagaaag ccgcagtgtc cgccatgcgc cgtggtcacg atgtccaggc
     3601 gcggcgagcg cgccagccgc gcgagatccc tggcctcgat gatggggtcg tcgagcgagg
     3661 tcagcacagt cgcaggcgcc gcgagagttg tcagacgatc gccagtgatc gcgtagccgg
     3721 cgaggtagtc gtccatgctc gggtattcgg tatgccgctc gacgagctgc cgcgtcattt
     3781 cgcgcaggct gcgaatgcga agcaggtcgt caaagttgta atggtcgggc caggctaact
     3841 gcttcttcgc cagcgaccgg gtccacttgc gaacgaagta gccgtggtac agcggggacc
     3901 cgcgttcgag tgtcgtcatg gtcaccgccg gatcgagaac cggcgacacg gcgagcacgc
     3961 gttgcagcgg gagatcgcga gcttcgcggt ccgcggcgac gcgcagcatg aagttgccgc
     4021 ccaacgaaaa cccgacgagc acgatcggca ggttcgcgaa ccgctgcgcc agcgaaagta
     4081 cggcgcccac tacttcgggc aggaggcagg agtgaaaaag atcgcggttc aggtgatgcg
     4141 tggttccgtg atcgcgcaga ttgaggcgca cgacttcgaa gtcccgctcg aacaacgtct
     4201 gcgcgaccga gagcacgtag gtggaatcag cactgccttc ccagccgtgc aacagcaccg
     4261 cgagagtttt gccgggttca cggccacact tggcggggct cgcgtgaaac gcctgcagga
     4321 cgacgccatc accgcattcg agcagcagtt c
//
LOCUS       DATDEU010000131         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1045317, whole genome shotgun sequence.
ACCESSION   DATDEU010000131 DATDEU010000000
VERSION     DATDEU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045317"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1056
                     /locus_tag="VIV63_03235"
     CDS             142..1056
                     /locus_tag="VIV63_03235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6123640.1"
                     /translation="MKHRASLVLLCALAAGAALAADPVDKKKPPTTAEVLESSQPSDW
                     RRPAPGQTLYLELAAGRVIIELAPSLAPRHVGVIRQLAAEKYYDGLPIARVQDNYVVQ
                     WGDVDEQRKIEPPLRPLPDESVVPWHERLGFVKLPDKDGYAPETGWIDGFPVGRNPRS
                     PATAWITHCYGVVGVGRNDPPDSGSGTELYVVIGQAPRHLDRNIVTVGRVLRGMEILS
                     SLPRGTGALGFYEKPEQRTPIKSIRVAMDVPEAERTNLEVLRTDTPLFTKFVEARRNR
                     REGWFTTPIGHIDVCNINIPVRDAPAPN"
     gene            1225..1947
                     /locus_tag="VIV63_03240"
     CDS             1225..1947
                     /locus_tag="VIV63_03240"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY6123641.1"
                     /translation="MGRIFEVRKHTMFARWDRMAKQFARISKDINMAVKAGGSDPNSN
                     PALRRAIQNSRNLNMPKDKVEAAIKRASGKDATAYEQIIYEGYAPHGIAILVEAATDN
                     PTRTVAQVRAILSKGGGNLGTTGSVAFQFRKMGVFRLKPDGIDHDDLELYLIDHGLEE
                     MGDSVGEKGEPQIVARCLFPEFGHMQEALEKRGIHPLSATHEYICTTPLELAEAQATE
                     VLELVDTLEQNDDVQNVYHSLV"
     gene            1954..>2318
                     /locus_tag="VIV63_03245"
     CDS             1954..>2318
                     /locus_tag="VIV63_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY6123642.1"
                     /translation="MEAIEALITRASAKTYGDTAPTPEHLATVLQAAVRAPDHGRLRP
                     WRFMLIEGDQRRKFGDMLAASALRRVPALSEGDLQRERDKALRAPLIIVVACRIVPGT
                     KVPAIEQILAAGAAAQNILL"
BASE COUNT          434 a          817 c          699 g          368 t
ORIGIN      
        1 cacacccatg agcgtgggcg gcgtcatcgc catcatgttc gtgtcgtcca tggtctgcct
       61 gtccaccgcg ttcctcatgt tcctcatcga ggtgcgcatc gccaccaaca cgctgcgcat
      121 cggcgttcag aagcacgaca aatgaagcac cgcgccagcc tcgtcttgct ctgtgccctg
      181 gctgccggcg cggcattggc tgccgacccg gtcgacaaga aaaagccacc gaccacggcg
      241 gaggtgctgg agtcttcgca gccttccgac tggcgtcgcc ctgctcctgg tcagactctc
      301 tacctggaac tcgcggccgg tcgcgtcatc atcgagctcg cgccatcgct cgcgccgcgg
      361 cacgtgggcg tcatccggca actcgccgcc gagaagtatt acgacggcct gcccatcgcg
      421 cgcgtgcagg acaactacgt cgtgcaatgg ggcgacgtcg atgaacagcg caagatcgag
      481 ccgccgttgc gaccgctgcc cgatgaatcg gtcgtgccgt ggcacgagcg cctgggcttc
      541 gtcaaactac ctgacaagga cggttacgcg cccgagaccg gctggatcga cggctttccc
      601 gtgggccgca accccaggtc gccggccacc gcgtggatca cgcattgtta cggggtcgtc
      661 ggcgtggggc gcaacgaccc gccggacagc ggcagcggca ccgaactcta cgtggtgatc
      721 ggccaggcgc cgcgacacct cgatcgaaac atcgtgaccg tcggccgcgt gttgcgcggc
      781 atggaaatcc tctcatcgct gccgcgcggc accggtgcgc tgggcttcta cgagaagccc
      841 gagcagcgca ctccgatcaa gtcgatccgc gtcgccatgg atgtaccgga agccgagcgc
      901 acgaatctcg aagtcctgcg caccgatacc ccgctcttca cgaagttcgt cgaagcccgg
      961 cgcaatcgcc gcgaggggtg gttcaccacg cccatcggcc acatcgacgt ctgcaacatc
     1021 aacatcccgg tgcgcgacgc acctgcccct aactagaaat ggggacattc ctgatttgct
     1081 agcaaatggg gacgcgcctc gaagggccgt caccgcccgg gctccactgt cgaaaagccc
     1141 cagccgcgtc cccttttgca gcaaatcagg aatgtcccca tttcgccttg ccattccgcg
     1201 ctttgctgcc agaattcgcg ccctatggga cgcatattcg aagttcgaaa acacacgatg
     1261 ttcgcgcgct gggaccgcat ggccaagcag ttcgcgcgca tctccaagga catcaacatg
     1321 gccgtcaagg ccggtgggtc cgacccgaat tccaatcccg cgttgcgccg tgcgatccag
     1381 aacagccgca acctcaacat gcccaaggac aaggtcgagg ccgccatcaa gcgcgcctcc
     1441 ggcaaggacg ccacggcata cgagcagatc atctacgagg gttacgcgcc gcatgggatc
     1501 gcgatcctcg tcgaagccgc cactgacaac cccacgcgaa cggtcgcgca ggtgcgcgcc
     1561 attctttcca aaggcggcgg caacctgggc acgacgggga gcgtcgcctt ccagttccgc
     1621 aagatgggcg tgttccgcct gaagcccgac ggcatcgacc acgacgacct cgagctctat
     1681 ctcatcgacc acggtctcga ggagatgggc gacagcgtcg gggagaaagg cgagccgcag
     1741 atcgtggcgc gctgcctgtt tccggagttc ggccacatgc aggaagcgct cgagaagcgc
     1801 ggcatccatc cgctgtccgc cacgcacgaa tacatctgca ccacgcccct cgagcttgcc
     1861 gaagcgcagg ccaccgaagt gctggagctc gtcgacacgc tcgaacagaa tgacgacgtg
     1921 caaaacgtct accattcgct cgtctagtta gccatggaag ccatcgaagc gttgatcacg
     1981 cgcgcctccg ccaagaccta cggcgacacg gcccccaccc cggaacacct cgcgaccgtc
     2041 cttcaggccg cggtgcgcgc gcccgatcac gggcggctgc ggccgtggcg cttcatgctc
     2101 atcgagggtg accagcgccg gaagttcggc gacatgctgg ccgcctcggc gctgcgccgc
     2161 gtccccgcgc tgagcgaagg cgatctgcaa cgcgagcgcg acaaggcgct gcgcgcgccg
     2221 ctcatcatcg tcgtggcctg ccgcatcgtg ccgggcacca aggtgccggc gatcgaacag
     2281 atcctcgccg cgggcgcggc cgcgcagaac atcctgct
//
LOCUS       DATDEU010000132        13761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1628133, whole genome shotgun sequence.
ACCESSION   DATDEU010000132 DATDEU010000000
VERSION     DATDEU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13761
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628133"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="VIV63_03250"
     CDS             <1..1424
                     /locus_tag="VIV63_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6123643.1"
                     /translation="YSVTIFEQWGVAGGLMRTNIPSFRLPAEVLNDEIAQIVEMGVDL
                     KLNHPITSLKELLTKGGFDAVFVGAGAPKGKELKLPGRAEGGANIHIGITWLESVAFE
                     HIKSIGEKVLIIGVGNTAMDCCRTSLRLGAKDVKVMARKPRQFFKASAWELEDAEEEH
                     VKIIINRSPKNFVIEGGKLKGMNFDVIEYDLDVKGAITAERVTGQEFIEADDVILAIG
                     QENSFPWIERDIGIEFDKWDVPKVDLKTFQSTRDGVFFGGDAAFGPKNIIWAVEHGHQ
                     AAISIHNLCQSKSVADRLPAGVNLQSTKMGMHEWAYANDYNPAERRLMPHVSLKERFK
                     KLNIEVELGFTIEQIEKEVQRCLNCDVQTVFETKLCIECDACIDICPVDCLTITHNGE
                     EADLRGRLKAPAKNVTQALYVSSALKHTGRVMVKDEDLCVHCGLCAERCPTAAWDMQK
                     STIQWPHATDEAAAAQAKLPKSA"
     gene            1426..3276
                     /locus_tag="VIV63_03255"
     CDS             1426..3276
                     /locus_tag="VIV63_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY6123644.1"
                     /translation="MPERVNNFAIKIATVNGTGSASANTLLMKSIFRSGVPVMGKNYF
                     PSNIQGLPTWYEIRVTKDSYVARSGKIDLMVAMNAETYTKDVREVTSGGYLIYDSTWP
                     RPALLARDDITVLGVPLAKLCNENFNGVRTRILMKNICYAGVLAALLDLDIAKIRELL
                     AETYAKKPALVDSNMKAIELGYNYTRENFQCPLPMHVAPGLLGASKTTGHVLIDGNTA
                     AGLGCVFAGATVGAWYPITPSTSLMDAYKMFSDKLRVDPETGKKNFAFIQAEDELAAI
                     GMVLGANWNGARAFTATSGPGLSLMNEFLGLAYYAEVPAVLFDIQRVGPSTGMPTRTQ
                     QCDILEGAYASHGDTKHVCLFPRDPEECFYFARDAFDLADRLQTPIMVMSDLDIGMND
                     WMCPDLKWDDHYVPDRGQVYNKDEIAKLEKFHRYLDVDGDGIPWRTLPGVHPKGAYFT
                     RGSGHTQYGVYTEDSAEYQIVLDRLTRKFATAKRLVPKPVIEQDAHIDIAVVAYGSSD
                     GAVREALDILADHGVKTNYMRLRSFPFGDEVEKFLEQQKLVFVVEQNRDAQMRSLLTL
                     ETRVEKAKLRSLLHYSGLPISSEFIVKGVLAEIEPKSRKLEAIPGKSGAA"
     gene            3273..4328
                     /locus_tag="VIV63_03260"
     CDS             3273..4328
                     /locus_tag="VIV63_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY6123645.1"
                     /translation="MTFLPKPKVRHPSLPKNSLGLTRRDYEGGLSTLCAGCGHDSITA
                     AIIEASWGIDIEPQNMVKLSGIGCSSKTTAYFVSGGHGFNSVHGRMPSIAMGANAANR
                     NLTYIGVSGDGDTLSIGLGQFCHAIRRNLNMAYIIENNGVYGLTKGQFSASADVGTKA
                     KKGEVNQQPPIDPVLLALTLGATFVARSFSGDKQQLVPLIQAALKHKGFALIDVLSPC
                     VTFNDHEGSTKSYQYTREHYDAAVHADFVPFQREIAADYAAGDVLPVVLHDGSRILLR
                     KLDAGYDPTNRDAATAAISERLKKGEFLTGLLYVDPEGKEFHDVNETPAIPLNQIPHT
                     RLSPGSAALAKVLARYR"
     gene            4378..4584
                     /locus_tag="VIV63_03265"
     CDS             4378..4584
                     /locus_tag="VIV63_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123646.1"
                     /translation="MAEEEKSQKFTSKVRRGLTWVSTVSSTELARVKQEAGGGRIPGM
                     TAAQLSDIEAALLWIAQNKEEANG"
     gene            complement(4786..6102)
                     /locus_tag="VIV63_03270"
     CDS             complement(4786..6102)
                     /locus_tag="VIV63_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123647.1"
                     /translation="MSGRPPFRSQHPAHVEEFWLLITIAPISAAIGLAFYGANSGRLW
                     LVAVAAAFGVAGTLIAWKSYERATTRKLERDRARADEIRQQDFGAGESFVVARRRSFV
                     RAIIATGVLVVAIISLSVLDLPAWKWAALGLLVPPTAIGWFLALRERGDILELSSSGI
                     RAAGHEVLWSDVANILHGYEWRDQGPYLRVLLNQPLPPQTLSRRIRSMLSRGESDREI
                     FVLLTRASESPAVIYDVALEVWGRMGGPAIRPAAQKIEAPRRPSFVVVAAALTIGVLF
                     IHGLSVDFLTSTAWSAWTGWVAAAVTILTLWWLHSCAGFPGILLSQRSTASYLMTLLV
                     MVPLMLLGSWAITARSLPDTATRWFGAQTQQVVLLHKVSRKSSRGCRLRITGELFFGR
                     TPPHYCVSGEEYERLPDRGPMKLTTTESWFGMHIHRIEPAEAATPE"
     gene            complement(6111..7208)
                     /locus_tag="VIV63_03275"
     CDS             complement(6111..7208)
                     /locus_tag="VIV63_03275"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEY6123648.1"
                     /translation="MSIQTRGAAITGWGKCLPPGVLTNADIATFLETSDEWITSRTGI
                     RERRVSHVGLGELMYVATARALACAGMDGKKIELIVAGTTSYDDQVPNVASGVQRRIG
                     ADGCAAMDVNTACTSFLYSLSTATALIKTGVVKNALVIGAEVISPFIDWSDRNTAVLF
                     GDGAAAVVLEATDLPEGLLAERLGCYGESREILRVHGMGAAYAHQNRIIGKTEWQFEG
                     QEIFKKAVHGMAMACGEALAKIGKTADDVDLVIPHQANMRIIESVAKKAGVPMERVFT
                     NVHRYGNMSAATVPVALCEALEEGRVKPGALLLMPSFGGGLTFTGHVVRWGQRVTPLG
                     VSDVELPPEQDTALERVAKFRAIKNLRVEAS"
     gene            7250..7882
                     /locus_tag="VIV63_03280"
     CDS             7250..7882
                     /locus_tag="VIV63_03280"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEY6123649.1"
                     /translation="MTEHNFSRWRPHPWHGLEVGEDAPRIVNAYIEITPFDMIKYEID
                     KVSGYLRVDRPQRSSSHPPALYGFIPRTYCGTRIANLAPGSERGDGDPLDICVISERP
                     IERSEVILRARVIGGIKLLDRGEADDKIIGVLEGDYVWDHVKDISQLAPVLVERLEHY
                     FGTYKMVPGEPNKMVISGKYGFDHAKAVIEAARADYEESFVDAMSERRRT"
     gene            complement(7879..8247)
                     /locus_tag="VIV63_03285"
     CDS             complement(7879..8247)
                     /locus_tag="VIV63_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123650.1"
                     /translation="MQTEAVNPTPPARGLRPGALLISGAINLLGALLVIFAMPGAASD
                     DRVASAWALVQFLSGVWAGVLGANSPFLHGLVAGLPALLLGLVLPSPLPKQFVVVAWF
                     LAPCAALLAAAIMRFMRRRA"
     gene            8592..10670
                     /locus_tag="VIV63_03290"
     CDS             8592..10670
                     /locus_tag="VIV63_03290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY6123651.1"
                     /translation="MLAISEIAAAAPKRIPTHEDIWLMKRVGAPQVSPDGRWLVVSVV
                     EPAYDDNAQLSDLWLIDTAARHSSRRLTSTRRPESGVIWSPDSSRIVFSAQRENDDVP
                     QIYSLDMRGGGEAHRLTNLSGGARNPSFSHDGRRLAFVSLMYPQAKDDAANKAMVDAY
                     RTRKSNARIFNGFPIRSWDHWLDERQVRIFIQELDGDGWPSGSPRDLLFGTALASSAG
                     FAGRQTDTGEELEVEFTPDNQSVVFGATTNRNAAAYAFTDSQLFVTSITGGEPKRITQ
                     GGHSWAQPRFTPDGRTLLAILETQGTSVYNASRIAAIPWPDTGGKYHIVTDGLDRSVG
                     TFAVSPDSREVYFTAEDSGNEKLYSTRLGGGTVQTIFGVEKGTYTNLTIPALAGKLAI
                     YANWESASAPGEIALVQPQSGRALILTKFNAARMAQLDLPPIENFWFTSSRGKRIHSF
                     VVRPPGFDSSKKYPLFVVIHGGPHGMWRDQFFVRWNYHLLAASGHVLLLTNYSGSTGF
                     GEAFSRSIQGDPLKGPGDEINEAADAAIKQYSFIDGTRQCAGGASYGGHLANWLQGTT
                     TRYKCLVSHAGLVNLEAQWGTSDTIYSREVNLGGPPWEKAPVWSEQNPIRYAAKFRTP
                     VLVTVGENDFRVPLNNTLEYWSALQRMQVPSRLVVFPDENHWIQKGEDSRLFYAEIAA
                     WLARWLKPAE"
     gene            10671..11441
                     /locus_tag="VIV63_03295"
     CDS             10671..11441
                     /locus_tag="VIV63_03295"
                     /inference="COORDINATES: protein motif:HMM:NF016973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HEY6123652.1"
                     /translation="MMSDDWDFYFARLNDAVSSIFVDLGIRDDVPVEARPWLLWVWVV
                     LQAPKDDGLATNEEGRVLHEIGESLDALVSATCGAQLVGRITGTGRREFYFYAAEPGE
                     LTTSVATAMKSFPQYKYETGSTFQPEWEQYLTLYPSETNLERMQNRRMLESLAKQGDA
                     HEVPRKIEHWLHFGDEPSRAACRDTLVAIDFAVEDEGMSEEEGAEMPFALVVSRVDSV
                     DTHTINGITLELARLAGEYCARYDGWECAVTRAEATST"
     gene            complement(11428..12033)
                     /locus_tag="VIV63_03300"
     CDS             complement(11428..12033)
                     /locus_tag="VIV63_03300"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY6123653.1"
                     /translation="MTPELLTSAAADRNKDPILTVLESVLPYSGSVLEIASGTGQHVC
                     FFAGELPLTRWQPTEPDAAAREAITIRIREAGLENVAQPIALNVLDPHWPVHERYDAV
                     LCINMIHISPWGATHALFRGVAKLLSTEGKLVLYGPYLENGTAVQSNLDFDASLKRRN
                     PEWGLRDLDEVTRVAAGHGFERQQVVRMPANNLTVVFVRST"
     gene            complement(12030..12209)
                     /locus_tag="VIV63_03305"
     CDS             complement(12030..12209)
                     /locus_tag="VIV63_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123654.1"
                     /translation="MKIAKPMLLVLTPLGVGWGLLEAWRFHWWLAVLMGALVSVISLF
                     MWMTVKRIRAERAPP"
     gene            complement(12219..13547)
                     /gene="hslU"
                     /locus_tag="VIV63_03310"
     CDS             complement(12219..13547)
                     /gene="hslU"
                     /locus_tag="VIV63_03310"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY6123655.1"
                     /translation="MTPRDIVSELDKHIVGQGAAKRSVAIALRNRWRRMQIEDPLRQE
                     ITPKNILMIGPTGCGKTEIARRLARLAKAPFVKVEATKFTEVGYVGREVDSIIKDLAE
                     VAVKMTRDEEIAKVRDRAADAAEERVLDALLPSPKMMGFSTDQEASPRDQETRQKFRK
                     MLREGALNDREIELDLRAIPPGVQIMAPPGMEEMQEQLSSMFANIGGQRTKPRKLKVR
                     EAQKLLIEEEAGKLVNEEELRARALANAEQNGIVFIDEIDKIARRQETMGADVSREGV
                     QRDLLPLVEGSTVSTKYGQIKTDHILFIASGAFHMSKPSDLIPELQGRFPIRVELNSL
                     GVDDFVRILTEPDASLTAQYKALMGTEKVEINFGADGVRRIAEIAFSVNEKTENIGAR
                     RLHTVMERLLETVSFDAAEQGGTRVDIDAAYVEQHLGNLAKDEDLSRYIL"
     gene            complement(13544..>13761)
                     /locus_tag="VIV63_03315"
     CDS             complement(13544..>13761)
                     /locus_tag="VIV63_03315"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939585.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEY6123656.1"
                     /translation="NLFVISGNGDVIEPDHDVVAIGSGGPFAQAAARALIENTELDAR
                     SVVERSLNIAADICIYTNRNLEIEELK"
BASE COUNT         2637 a         4405 c         4333 g         2386 t
ORIGIN      
        1 gttactccgt cacgatcttc gaacagtggg gagtcgcggg cggcctcatg cgcaccaaca
       61 tcccctcgtt ccgcctgccc gccgaagtgc tgaacgacga gatcgcgcag atcgtcgaga
      121 tgggcgtcga cctcaagctc aatcacccga tcacgtcgct gaaggagctg ctcaccaagg
      181 gcggcttcga tgccgtgttc gtgggcgccg gcgctccgaa gggcaaggaa ctcaaactac
      241 cgggacgcgc cgaaggcggc gccaacatcc acatcggcat cacctggctc gagtcggtgg
      301 cgttcgagca catcaagtcc atcggcgaga aggtgctcat cattggtgtg ggcaacacgg
      361 ccatggactg ctgccgcacg tcgttgcgcc tcggcgccaa ggacgtgaag gtcatggcgc
      421 gcaagccgcg gcagttcttc aaggcttccg cgtgggagct cgaggatgcc gaagaagagc
      481 acgtcaagat catcatcaac cgctcgccga agaacttcgt gatcgaaggt ggcaagttga
      541 agggcatgaa cttcgacgtc atcgagtacg acctcgacgt gaagggcgcc atcaccgccg
      601 agcgggtaac gggccaggaa ttcatcgagg ccgacgacgt catcctcgcc atcggccagg
      661 agaattcctt cccctggatc gagcgcgaca tcggcatcga gttcgacaag tgggacgtcc
      721 ccaaggtcga cctcaagact tttcagtcga cgcgcgatgg cgtgttcttc ggcggcgacg
      781 ccgcgttcgg ccccaagaac atcatctggg ccgtggagca cgggcaccag gcggccatct
      841 ccattcacaa tctctgccag tcaaagagcg tcgccgatcg cttaccggca ggtgtgaacc
      901 tgcagtccac caagatgggc atgcacgaat gggcttacgc caacgactac aacccggccg
      961 agcggcggct catgccgcat gtctcgctga aagaacgctt caagaaactc aacatcgaag
     1021 tcgagctggg tttcacgatc gagcagatcg agaaagaggt gcagcgctgt ctcaactgcg
     1081 acgtgcagac ggtgttcgaa accaagctgt gcatcgagtg cgacgcgtgc atcgacatct
     1141 gtccggtgga ctgcctcacc atcacacaca acggtgaaga ggcggacctg cgcgggcggc
     1201 tcaaggcgcc ggcgaagaac gtcactcagg ctttgtacgt atcgtccgcg ctgaaacaca
     1261 cgggccgcgt catggtcaaa gacgaagacc tgtgtgtgca ctgcgggctt tgcgcggagc
     1321 gctgtccgac cgcggcctgg gacatgcaga aatccacgat ccagtggcca catgccacgg
     1381 acgaagccgc tgctgcgcaa gccaagcttc cgaagagcgc ttgagatgcc agaaagagta
     1441 aacaacttcg cgatcaagat cgcgacggtg aacggtaccg gttccgccag cgccaacacc
     1501 ctgctcatga agtcgatctt ccgcagcggc gtgcccgtca tgggcaagaa ctacttcccc
     1561 tcgaacatcc agggcctgcc cacctggtac gagatccgcg tcaccaaaga ttcgtatgtc
     1621 gcgcgctcgg gaaagatcga cctcatggtc gcgatgaacg ccgagacata tacgaaggac
     1681 gtgcgcgaag tcacttccgg cggctacctc atatacgact ccacctggcc ccggcccgcc
     1741 ttgttagccc gcgacgacat cacggtgctc ggcgtgccgc tggcgaagct ctgcaacgaa
     1801 aacttcaacg gcgtgcgtac gcgcatcctc atgaagaaca tctgttacgc cggcgttctc
     1861 gcggcgttgc tcgacctcga catcgcgaag atccgcgagc tgctcgccga aacctacgcg
     1921 aagaagccgg cgctcgtgga ctcgaacatg aaggccatcg agctcggcta caactacacg
     1981 cgcgagaact tccagtgccc gctgcccatg catgtggcgc ccggattgct cggcgcctcg
     2041 aagaccacgg gccacgtcct catcgacggt aataccgcgg cgggcctggg ctgcgtgttc
     2101 gcgggcgcga ccgtaggcgc ctggtatccg atcacgccgt ccacctcgct catggacgcg
     2161 tacaagatgt tctcggacaa actgcgcgtg gaccccgaga ccggcaagaa gaacttcgcg
     2221 ttcatccagg ccgaagacga actggccgcc atcggcatgg tgctgggcgc caactggaac
     2281 ggcgcgcgcg cgtttaccgc gacctcgggt cctgggctct cgctgatgaa cgaattcctg
     2341 ggcctcgcgt attacgccga agtgcccgcg gtgttgttcg acatccagcg cgtgggtcct
     2401 tcgaccggca tgcccacgcg cacgcagcaa tgcgacatcc tcgagggcgc ttacgcgtcg
     2461 cacggcgaca ccaaacacgt ctgcctgttc ccgcgcgatc cggaggagtg tttctatttc
     2521 gcgcgcgacg ccttcgatct cgccgaccgt ctgcagacgc ccatcatggt catgtcggat
     2581 ctcgacatcg gcatgaacga ctggatgtgc cccgacctga agtgggacga ccactacgtt
     2641 cccgatcgcg gccaggtcta taacaaggac gagatcgcca agctcgagaa gtttcaccgc
     2701 tatctcgacg tggacggtga cggcattccc tggcgcacgc tgccgggcgt gcatcccaaa
     2761 ggcgcgtatt tcacgcgcgg ctccggccac acgcagtacg gcgtgtacac ggaagattcc
     2821 gccgaatacc agatcgtgct cgaccgcctg acgcgcaagt tcgccaccgc caaacggctg
     2881 gtgccgaagc cggtcatcga gcaggacgcg cacatcgaca tcgccgtggt cgcctacggc
     2941 agctccgacg gcgcggtgcg cgaggcgctc gacatcctcg ccgatcacgg cgtcaagact
     3001 aactacatgc ggttgcgctc gttcccgttc ggcgacgaag tggagaaatt cctcgagcag
     3061 cagaagctgg tgttcgtcgt cgagcagaac cgcgatgcgc agatgcgttc gctgctcacg
     3121 ctggagacgc gcgtggagaa agccaaactg cgttcgctgc tgcactacag cggactgccg
     3181 atttcctccg agttcatcgt caagggtgtg ctcgccgaga tcgagcccaa gtcccgcaag
     3241 ctcgaagcca tccctggcaa gtcaggtgcc gcatgacatt cctgcccaaa cccaaggtcc
     3301 gtcacccctc gctgccgaag aattcgctcg gcctcacgcg gcgcgattat gaaggtgggc
     3361 tgtccacgct ctgcgccggc tgcggccacg actcgatcac cgcggcaatc atcgaggcct
     3421 cctggggcat cgacatcgag ccgcagaaca tggtcaagct gtcgggcatc ggctgctcgt
     3481 cgaagaccac ggcctatttc gtgagcggcg ggcatggctt caattccgtg catggccgca
     3541 tgccttccat cgccatgggc gccaatgcgg cgaaccgcaa tctcacgtac atcggtgtct
     3601 ccggcgacgg cgataccctg tccatcgggc tcgggcagtt ctgccacgcg atccgccgca
     3661 acctcaacat ggcctacatc atcgaaaaca acggtgtgta cggcctgacg aaggggcagt
     3721 tctcggcatc cgcggacgtg ggcaccaagg cgaagaaagg cgaggtgaat caacagccgc
     3781 cgatcgaccc cgtgctgctt gcactgaccc tgggcgccac gttcgtcgcg cgcagttttt
     3841 ccggcgacaa acaacaattg gtgccgctca tccaggctgc gctcaaacac aagggtttcg
     3901 cgctcatcga cgtcctgtct ccctgcgtca cgttcaacga tcacgaaggg tccaccaaga
     3961 gttatcagta cacccgcgag cactatgacg ccgcggtgca cgcggacttc gtgccgttcc
     4021 agcgtgagat cgccgccgac tacgccgcgg gcgatgtttt acccgtcgtg ttgcatgacg
     4081 gcagccggat tctgctgcgc aaactcgatg cggggtacga cccgacgaat cgcgatgcgg
     4141 cgaccgccgc gatatcagaa aggttgaaaa aaggtgaatt cctgacggga cttctatacg
     4201 tcgacccgga aggaaaggag tttcacgatg tcaatgagac accggctatt ccgttaaacc
     4261 agattcccca tacccgactg tcgccagggt cggcggcatt ggcgaaagtt ctggccagat
     4321 atcgttagcc gcgctatgtt tcggcccgct atagagatcg gttgatcagg agtcgacatg
     4381 gccgaagaag agaagtccca gaaattcacc tccaaagtgc gccgcgggct cacttgggta
     4441 agcacggtgt cgtcgaccga gctcgcgcgc gtcaagcagg aagccggcgg tggccgtatc
     4501 cctggcatga ctgccgcaca gctctccgac atcgaagctg cgctgctatg gattgcgcag
     4561 aacaaggaag aagcgaatgg gtaagggtga ccgccgcacg aagcgcggaa agttgtacgc
     4621 gggctcgttt ggaaagtccc gcccgaaaga tccagccaag aaaaaagcaa aagccaaagc
     4681 gaagaagaaa gcctaggaaa gtaaacctag taaagaaagc caagagccag acgttcttag
     4741 cacaggtcga acgaagcccg cgaagcgcaa gcaacgcggg ctttgtcatt ctggggtggc
     4801 ggcttcggcc ggctcgatgc gatggatgtg catgccgaac cacgattccg tcgtggtcag
     4861 cttcatcggc ccgcggtccg gcagccgctc gtactcttcg ccgctcacgc agtagtgcgg
     4921 cggtgtgcgg ccaaaaaaca gttctccggt gattcgcaat cggcagcctc tcgagctctt
     4981 tcgcgacacc ttgtgcagga gcacgacctg ttgcgtctgc gcgccgaacc agcgcgtggc
     5041 cgtgtcgggc aatgaccggg cggtgatggc ccaactcccg agcaacatca gagggaccat
     5101 gaccagcaag gtcatcagat agctcgcggt gcttcgttgg ctgagcagaa ttccgggaaa
     5161 tcccgcgcaa gagtgcagcc accacaacgt gaggatcgtg accgctgcag cgacccaccc
     5221 ggtccaggcc gaccacgccg tcgaggtcag gaaatcgacg ctcaggccgt ggatgaagag
     5281 gacgccaatg gtgagggccg ccgccaccac gacgaagctc ggtctccgtg gcgcctcgat
     5341 cttctgcgcg gcaggccgga tcgcgggccc acccatgcgt ccccacacct ctaacgcaac
     5401 gtcgtagatg accgccgggg attcagacgc acgcgtcagc aggacgaaaa tttcgcgatc
     5461 cgattcgccg cgcgacaaca tcgaccggat gcgccgcgaa agcgtttgcg gtggcaatgg
     5521 ctggttgagc aacacacgga ggtaaggacc ctggtcgcgc cactcgtatc catgaaggat
     5581 gtttgcgacg tcactccaga gaacttcatg gccagcggcg cggatacccg acgacgacag
     5641 ttcgaggatg tcgccacgtt cgcgcagtgc gagaaaccag ccgatcgccg tcggcggcac
     5701 caacaagccc agcgcggccc atttccacgc gggcaagtcg aggacactca gtgagatgat
     5761 cgccaccacg agaacgccgg tggcaatgat ggcgcggacg aacgatctcc gtcgtgcgac
     5821 cacgaacgat tcccccgcgc cgaaatcctg ctgcctgatc tcgtcggcgc gggcgcgatc
     5881 acgttccagc tttcttgtgg tggcacgttc gtagctcttc caggcgatga gtgttccggc
     5941 gacgccgaat gcggcggcga ccgcgacgag ccagagccgg ccgctgtttg cgccgtagaa
     6001 agcgagcccg atcgcggcgg agatcggcgc gatcgtgatg agcagccaga attcttcaac
     6061 gtgagcggga tgctgcgaac ggaaaggtgg gcgcccgctc atgggcgcgc tcaggatgcc
     6121 tcgacgcgaa ggttcttgat cgcgcgaaat ttcgcgacac gttcgagcgc ggtgtcctgt
     6181 tcgggcggaa gttcgacgtc gctgactccg agcggcgtca cgcgctgacc ccagcgcacg
     6241 acgtggcccg tgaacgtgag cccgccgccg aacgaaggca tgagcagcag cgcgccgggc
     6301 ttcacgcggc cttcttccag cgcctcgcac agcgccacgg gcaccgtggc cgccgacatg
     6361 ttgccgtagc ggtgcacgtt ggtgaacacg cgctccatgg gcacgccggc cttctttgcc
     6421 accgattcga tgatgcgcat attggcctgg tgaggaatca cgaggtcgac gtcgtcggcc
     6481 gtcttgccga tcttggcgag tgcttcgccg caagccatgg ccatgccgtg tacggccttc
     6541 ttgaagattt cctggccttc gaactgccac tcggtcttgc cgatgatgcg gttctggtgg
     6601 gcataggcgg cgcccatgcc gtgcacgcgc aggatctcgc gcgattcgcc gtaacagccg
     6661 agacgctcgg ccagcaggcc ttccggaaga tcggtggcct cgagcaccac ggccgcggcg
     6721 ccgtcgccga acagcaccgc ggtgttgcga tccgaccagt cgatgaacgg cgagatgacc
     6781 tcggcaccga tgaccagcgc gttcttgacc acgccggttt tgatgagcgc cgtggcggta
     6841 gatagggaat agaggaagct ggtgcaggcg gtattcacgt ccatcgcggc gcagccgtcg
     6901 gcgccgattc gccgctggac accggaagcc acgttcggca cctggtcgtc gtagctggtg
     6961 gtgccggcca cgatgagttc gatcttcttg ccgtccatgc ccgcgcaggc gagggcgcgc
     7021 gcggtggcca cgtacatgag ttcgcccagg ccgacatgcg aaacgcggcg ttcgcggatg
     7081 ccggtgcgcg aggtgatcca ctcgtcgctg gtctcgagga aagtggcgat gtcggcgttc
     7141 gtgaggacgc ccggaggcaa gcacttgccc cagccggtga tggccgcacc cctcgtttgt
     7201 atggacatgg gctgggagga tatgcggaac tcccgtagag ttcacgccca tgaccgaaca
     7261 caacttttcc cgatggcgcc cgcatccctg gcatggcctg gaggtgggcg aggacgctcc
     7321 ccgcatcgtc aacgcctaca tcgaaatcac cccgttcgac atgatcaagt acgagataga
     7381 caaggtctcg ggctacttgc gggtcgaccg gccgcaacgc tcttcgtccc acccgccggc
     7441 gttatacggg ttcattccca ggacctattg cggcacgcgt atcgcgaacc tggcgccggg
     7501 gtccgagcgc ggggacggtg atccgctcga catctgcgtg atcagcgaac gtcccatcga
     7561 gcggtccgag gtcatcctgc gcgcgcgcgt catcggtggc atcaagctgc tcgaccgcgg
     7621 cgaggccgac gacaagatca tcggcgtgct ggaaggggat tacgtgtggg accacgtgaa
     7681 ggacatctcg cagctcgcgc ccgtgctcgt cgagcggctg gagcattact tcggcaccta
     7741 caagatggtg ccgggcgaac ctaacaagat ggtgatctcg ggcaagtacg gcttcgatca
     7801 cgccaaggcg gtcatcgaag ccgcgcgcgc cgactacgag gaaagtttcg tcgacgcgat
     7861 gtcggagcgc agacggactt aagcgcggcg gcgcatgaag cgcatgatcg ccgcggccag
     7921 caacgcggca cacggcgcca ggaaccacgc gacgaccacg aactgtttgg gcaacgggct
     7981 cggcagcacg agtccgagca acagcgccgg caggcccgcg acgagtccgt gcaggaacgg
     8041 agagttggcg ccaagtacgc cggcccagac tcccgacagg aattgcacca gcgcccatgc
     8101 cgacgccaca cgatcgtcac ttgcggcgcc cggcatcgcg aaaatgacga gcaacgctcc
     8161 gagcaggttg atcgcgccgc tgatcagcag cgcgccaggg cgcaggcctc gcgcgggcgg
     8221 agtcgggttt acggcttcgg tttgcaaatg aatctcttcc tgtcagaggc gcgaacactg
     8281 tcccgatcga ggaccgatag atagacgagg ccgcgttcgc cactcgccag gcgcgacggt
     8341 gcggcaatgt ccattgaaca aaagttgcac ggtacgaaac atgccgcaca cctcgccggg
     8401 tcgacgcggg atatggcaag cacccgctcg agcgcgctcg acgggttttt cacggcgcgt
     8461 agtctcgcgt tcgcgccggg ccgaggcaag tcatgcacca tcgggccgcg gtttgcgcta
     8521 agttgtacaa catgcaacat ctcgtaaacc ggcctgtaga ccggactatg gcgtgtttgt
     8581 ttgcctgtct gatgcttgcg atttccgaga tcgcagccgc ggcgcccaaa cgcatcccca
     8641 cacacgaaga catctggctc atgaagcgcg tgggcgctcc ccaggtgagt cccgatggcc
     8701 gctggctcgt cgtatcggtg gtcgaaccgg catatgacga caacgcgcag ctcagcgatc
     8761 tgtggctcat cgatacggcc gcgcggcatt cgagccggcg gctgacctcg acgcgccgtc
     8821 cggagagcgg cgtcatctgg agcccggaca gcagccgcat cgtgttcagc gcgcagcgcg
     8881 agaacgacga tgtgccgcag atctattcac tcgatatgcg cgggggcggc gaagcccatc
     8941 gcctcaccaa cctgtccggc ggcgcccgca acccctcgtt ctcgcatgac ggccggcggc
     9001 tggctttcgt gtcgctcatg tatccgcagg cgaaggacga cgcggctaac aaggcgatgg
     9061 tcgacgccta tcgcacccgc aaatccaatg cgcgcatctt caacggattc ccgatccgaa
     9121 gctgggacca ctggctggac gaacgccagg tgcgcatatt catccaggaa ctggacggcg
     9181 acggttggcc ttcgggcagt ccgcgcgacc tgctgttcgg caccgcgctg gcgtccagcg
     9241 ccgggttcgc cggccggcag accgacaccg gcgaagagct cgaagtcgaa ttcacgcccg
     9301 acaaccagtc cgtcgtcttc ggcgcgacca cgaaccgcaa tgccgccgcc tacgcgttca
     9361 ccgactctca gctgttcgtc acgagcatca ccggcggtga gccgaagcgg atcacccagg
     9421 gtgggcacag ctgggcccag ccacggttca cgcccgacgg ccgcacgttg ctggcgatcc
     9481 tcgaaacgca ggggacgagt gtctacaacg catcgcgcat cgcggcgatt ccctggcccg
     9541 acacgggtgg caaataccac atcgtcaccg acggcctcga tcgctcggtg ggcacgttcg
     9601 ccgtgagtcc cgattcgcgc gaggtctact tcaccgccga ggacagcggc aacgagaagc
     9661 tgtattccac gcgcctgggc ggcggcacgg tgcagacgat cttcggcgtc gagaaaggca
     9721 cctacaccaa cctcacgatc ccggcgctcg ccggcaagct cgcgatctac gccaattggg
     9781 aaagcgcgag cgcgcccggt gaaatcgcgc tcgtccagcc gcagagcggc agggcgctga
     9841 tcctcaccaa gttcaacgcg gcgcgcatgg cgcagctgga tctgccgccc atcgaaaact
     9901 tctggttcac gagcagccga ggcaagcgca tccacagctt cgtggtgcgg ccgccgggtt
     9961 tcgattcgtc gaagaaatat ccgctgttcg tggtcatcca cggcggcccg catggcatgt
    10021 ggcgcgacca gttcttcgtg cgctggaact accaccttct ggcggcctcc ggccatgtgc
    10081 tgctgctcac taactactcc ggctcgaccg ggttcggcga ggcgttctcg cgatccatcc
    10141 agggggatcc tctcaagggt cccggggatg agatcaacga ggcggccgat gcggcgatca
    10201 aacaatattc gttcatcgac ggcacccgcc agtgcgccgg tggcgccagt tatggcggcc
    10261 acctcgccaa ctggctccag ggcacgacga ctcgctacaa gtgtctggtg agtcacgccg
    10321 gcctcgtgaa cctggaggca caatggggca ccagcgatac catctattcg cgcgaggtga
    10381 atctgggtgg gccgccatgg gaaaaagcgc cggtgtggag cgaacagaat ccgatccgtt
    10441 atgccgcgaa gtttcgtacg ccggtactgg tcacggtcgg tgaaaacgac ttccgtgtgc
    10501 cgctcaacaa cacgctcgaa tactggagcg cgctgcagcg catgcaggtg cccagccggc
    10561 tcgtcgtttt ccccgacgag aatcactgga tacagaaggg tgaagacagc cggctcttct
    10621 acgcggaaat cgccgcgtgg ctcgcgcgct ggctcaaacc cgcggaatga atgatgtctg
    10681 acgattggga tttctatttc gcgcggctca acgatgcggt gtcgtcgatc ttcgtcgacc
    10741 tcggaatccg cgatgacgtg cccgtcgagg cccggccctg gttgctgtgg gtgtgggtgg
    10801 tgttgcaggc gccgaaggac gatggactcg cgaccaacga ggaaggccgc gtgctgcacg
    10861 agatcggcga atcgctcgat gcgctggtgt ccgccacctg cggcgcgcag ctcgtgggac
    10921 gcatcaccgg caccgggcgc cgtgagttct acttctacgc cgccgaaccg ggcgaactca
    10981 ccacctcggt tgccaccgcg atgaagtcct tcccgcagta caagtacgaa acgggcagca
    11041 ccttccagcc cgagtgggag cagtacctca cgttgtaccc ttcagaaacg aatctcgagc
    11101 gcatgcagaa ccgccgcatg ctcgagtcgc tggccaagca gggcgacgcg cacgaagtgc
    11161 cgcgcaagat cgaacactgg ctgcatttcg gcgacgaacc ctcgcgcgcc gcctgccgcg
    11221 acacgctcgt ggccatcgat ttcgccgtcg aggacgaagg gatgtccgaa gaggaaggcg
    11281 ctgaaatgcc gttcgcgctc gtggtgtcgc gcgtcgacag cgtggacacg cacaccatca
    11341 atggcatcac gctggaactg gcgcgcctcg cgggcgagta ctgcgcacgt tacgacggct
    11401 gggaatgcgc cgtgacgcgc gccgaagcta cgtcgacctg acgaacacca cggtgaggtt
    11461 gttagccggc atccgtacga cctgctggcg ctcgaacccg tggcctgccg ccacgcgcgt
    11521 gacttcgtcg aggtcgcgca gcccccattc cgggttgcga cgcttcagtg acgcgtcgaa
    11581 gtcgaggttc gactgcactg cggtgccgtt ctccaggtag ggaccgtaga gaaccagctt
    11641 cccttccgtg ctcaggagct ttgcgacgcc gcggaacagc gcgtgggtcg cgccccaggg
    11701 cgagatgtgg atcatgttga tgcacaacac cgcgtcgtaa cgctcgtgca cgggccagtg
    11761 ggggtcgagc acattcagcg cgatcggctg ggcgacgttc tcgagcccgg cctcacggat
    11821 gcggatcgtg atcgcctcgc gcgcggccgc gtccggctcg gtgggctgcc agcgcgtgag
    11881 cggcagttcg cccgcgaaaa aacagacgtg ctggcccgtg cccgaggcga tctcgagcac
    11941 gctcccgctg tacgggagca cggactccag gaccgtgagg atcggatcct tgttgcgatc
    12001 cgccgcggcc gacgtcagca gttcgggggt catgggggcg cacgctcggc gcggatgcgc
    12061 ttcacggtca tccacatgaa gaggctgatg accgacacca gcgcgcccat gagcaccgcc
    12121 agccaccagt ggaaacgcca cgcctccagc aaaccccagc cgacgccgag cggcgtcagc
    12181 actaacaaca tgggtttggc gattttcacc gggcgactct agaggatgta gcggctgagg
    12241 tcctcatcct tggcgagatt gccgaggtgc tgttccacgt aggccgcgtc gatgtccacg
    12301 cgcgtgccgc cttgctccgc cgcgtcgaac gacacggtct cgagcagccg ctccatgacg
    12361 gtgtgcagcc ggcgcgcgcc gatgttctcg gttttctcat tgacgctgaa ggcgatctcc
    12421 gcgatgcgac gcacgccatc agccccgaag ttgatctcca ccttctcggt gcccatgagc
    12481 gccttgtact gcgcggtcag cgacgcgtcg ggctcggtga ggatgcgcac gaagtcgtcc
    12541 acacccaggg aattgagctc cacgcggatc gggaaacggc cctgcaactc agggatgaga
    12601 tccgacggct tcgacatgtg gaacgcaccg gacgcgatga agaggatgtg atccgtcttg
    12661 atctgcccgt acttcgtcga cacggtgctg ccctcgacca gcggcagcag atcgcgctgc
    12721 acgccttcgc gtgacacgtc ggcgcccatg gtttcctggc ggcgcgcgat cttgtcgatc
    12781 tcgtcgatga acacgatgcc gttctgctcg gcgttcgcca gagcgcgggc acgcagctct
    12841 tcttcgttca cgagtttgcc ggcttcctct tcgatgagca gcttctgcgc ttcgcgcacc
    12901 ttgagcttgc gcggcttggt gcgctgtccg ccgatgttgg caaacatgct cgagagctgt
    12961 tcctgcatct cttccatgcc gggcggcgcc atgatctgga cgccgggcgg aatggcgcgc
    13021 agatcgagct cgatctcgcg gtcgttcagc gcgccttcgc gaagcatctt gcggaatttc
    13081 tgccgcgttt cctgatcgcg cgggctggct tcctgatcgg tggagaagcc catcatcttg
    13141 ggcgaaggca gcagcgcgtc gagcacgcgc tcttcggccg catcggcggc gcggtcgcgc
    13201 accttggcga tctcctcgtc gcgcgtcatc ttcacggcga cttcggcgag gtccttgatg
    13261 atggaatcga cctcgcggcc gacgtacccg acctcggtga acttggtggc ttccactttc
    13321 acgaacggcg ccttggcgag ccgcgcgagt cgccgcgcga tttcggtctt gccgcagccc
    13381 gtggggccga tcatgaggat gttcttgggc gtgatctcct ggcgcagcgg atcttcgatc
    13441 tgcattcggc gccagcgatt gcgcagcgcg atggcgaccg agcgcttggc ggcgccctgg
    13501 ccgacgatgt gtttgtcgag ttcggaaacg atgtcgcgcg gggtcacttc aattcctcga
    13561 tctccagatt gcgattggtg tagatgcaga tgtcggcggc gatgttgagt gagcgctcga
    13621 cgacgcttct ggcatcgagc tcggtgttct cgatgagcgc gcgcgccgca gcctgcgcga
    13681 agggaccacc ggagccgatg gccactacgt cgtggtcggg ctcgatgacg tcgccattgc
    13741 ccgagatcac gaacaggttc t
//
LOCUS       DATDEU010000133         2738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1190847, whole genome shotgun sequence.
ACCESSION   DATDEU010000133 DATDEU010000000
VERSION     DATDEU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190847"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VIV63_03320"
     CDS             complement(<1..1845)
                     /locus_tag="VIV63_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6123657.1"
                     /translation="MKITDDSPPTLSALDRPNSYIGRSVPRPNLARLTRGRAQFVSDV
                     TLPRMAHVAFVRSPHAHARITAIATAAAKTSPGVIAVVTGAELAKVITPWVGVLTHLK
                     GIKSAPQHAIAVERACWQGEAVCAVVARTRAEAEDGCELVDVGYEELPAVTDAETALD
                     AGTPVIHPELGDNLTFERVLVAGDPDQGFKDADAVVETTFVFGRHTGVTNEARAIVAD
                     WNSGDERLTVYQGTQAPHMMQNLFAKHLGLEEHQVRVLTKDVGGSFGIKVHTYADEMA
                     TVALSKLLKRPIKFVADRIESFVTDIHARDHRVKAKIGVKSDGSITAWEIDDLTGIGP
                     YSVYPRTSGIEANQIVNLTGGPYTCPNYRARARVVFQNKNVMCQYRAVGHPIATAVTE
                     GLVELAAAQIGMDPLEIRRRNLIPDDAHPSSGPSGIKFEALSQHESLAHLDAMMNYAG
                     LRAEQKRLRAQGIHRGIGFASFVEVTNPSAAFYGIGGAKISAQDGVTIRLDAQGAIIC
                     QTGLTEQGQGAEAVIAQVAASSFGVPIERVRIITGDTDNTPYGGGTWASRAAGIGGEA
                     AWQAGKALRANVLACAASILQAKPENLDIRNGAIVDSGTGTERLPLHEL"
     gene            complement(1842..2336)
                     /locus_tag="VIV63_03325"
     CDS             complement(1842..2336)
                     /locus_tag="VIV63_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6123658.1"
                     /translation="MNAQVHDIVMSVNGEETRATVDARKTLVDFLRDDAALTGSHIGC
                     EHGVCGACSVRVDGAVVRGCLMLAVQCDGARVETIEGVAETDEITDLQEAFIARNALQ
                     CGYCTPGMLLTAQELLAAGGVPSREQIRVYLSGNYCRCTGYQAIVDAVEAVAKARHSS
                     QGTR"
     gene            complement(2333..>2738)
                     /locus_tag="VIV63_03330"
     CDS             complement(2333..>2738)
                     /locus_tag="VIV63_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123659.1"
                     /translation="ADDFFKGLFETALTTRDVLTSIRIPAAGAQTRVGFAEFARRHGD
                     YAMVGLAASGRAYGKRLLDLRLAYFGVGSTPVRAHHAEPALADGDIDAAVRALAQDLN
                     PPDDVQASGAVKTHLAGVLLRRVARQLDRAWS"
BASE COUNT          440 a          886 c          933 g          479 t
ORIGIN      
        1 gagctcatgc aacgggaggc gctccgtacc ggtgccggaa tcgacaatag cgccattgcg
       61 gatgtcgagg ttttcgggct tggcctgcaa gatcgacgcg gcgcaggcga gcacgttggc
      121 gcgtagcgcc ttgccggcct gccaggccgc ttccccgccg atgccggccg cacgcgaagc
      181 ccaggtgccg ccgccatagg gcgtgttgtc ggtgtctccg gtgatgatgc gcacgcgctc
      241 gatcggcacg ccgaaggagg aggccgcgac ctgcgcgatg acggcttcgg cgccctggcc
      301 ctgctcggtc aagccggtct ggcagatgat cgcgccctgc gcgtcgagcc tgatggtgac
      361 gccgtcctgt gccgagatct tcgcaccgcc gataccgtag aaggcggcgc tcggattggt
      421 cacttcgacg aaggacgcaa atccgatgcc gcgatggatg ccttgcgcgc ggagccgttt
      481 ctgttcggcg cgcagcccgg cgtagttcat catggcatcg agatgagcaa ggctttcgtg
      541 ctgtgacagc gcctcgaact tgatgcccga gggaccgctg gacggatggg cgtcgtcagg
      601 aatgagattg cggcgccgga tttccagcgg gtccatgccg atctgcgcgg ccgcaagctc
      661 gaccagtccc tccgtcaccg cggttgcgat cggatggccg accgcacggt actggcacat
      721 gacgttcttg ttctggaaca ccacgcgggc tcgggcgcga tagttcgggc aggtatacgg
      781 ccccccggtg agattgacga tctggttggc ctcgatgccc gaggtgcgcg gatagaccga
      841 gtaaggaccg atgccggtga ggtcatcgat ctcccaggcg gtgatgctgc cgtcgctctt
      901 gacgccgatc ttcgccttca cgcggtgatc gcgggcatgg atatcggtga cgaagctctc
      961 gatgcgatcg gcgacgaact tgatcggacg cttgagcagt ttcgaaagcg caaccgtcgc
     1021 catctcgtcg gcataagtat ggaccttgat gccgaacgag ccgccgacgt ccttggtcag
     1081 gacgcggacc tgatgctcct cgagtccgag gtgcttggcg aacaggttct gcatcatgtg
     1141 cggagcctgg gtgccttgat agacggtgag ccgctcgtcg cccgagttcc aatcggcgac
     1201 gatggcgcgc gcctcattgg tgaccccggt gtgacgcccg aacacgaacg tcgtctccac
     1261 caccgcatcg gcgtccttga agccctggtc ggggtcgccg gcgacaagca cgcgttcgaa
     1321 ggtgagattg tcgccaagtt cgggatggat gactggcgtg ccggcgtcga gcgcggtctc
     1381 ggcatcggtg accgccggca attcctcgta gccgacatcc accagctcgc agccatcctc
     1441 ggcttcggcc cgtgtgcgtg cgaccacagc gcataccgcc tcgccttgcc agcacgcgcg
     1501 ctcgaccgca attgcatgct gcggcgccga tttgatgccc ttgaggtggg tgagaacgcc
     1561 gacccagggc gtgatgacct tcgccaattc agcgccggtc accaccgcga tgacgcccgg
     1621 cgacgtcttc gccgcggccg tcgcgatcgc cgtgatgcgc gcgtgggcgt ggggcgagcg
     1681 gacgaaggcc acgtgcgcca tgcgcggcag cgtcacatca ctcacgaact gggcgcgacc
     1741 acgagtgagc cgcgcgagat tgggccgcgg caccgatctg ccgatatagg agttcggccg
     1801 gtcgagggcc gagagcgtag ggggcgagtc gtcggtgatt ttcatcgcgt tccctgcgaa
     1861 gagtgccgcg ccttcgcgac ggcttccacc gcatccacga tcgcctgata gccggtacag
     1921 cggcagtagt tgccggagag atacacgcgg atttgttccc ggcttgggac gccgccggcg
     1981 gccagcaact cctgcgccgt cagcagcatg cccggcgtgc agtagccgca ctggagtgcg
     2041 ttgcgcgcga tgaatgcctc ctgcagatcg gtgatctcgt ccgtttcggc cacgccctcg
     2101 atcgtctcga cgcgcgcacc gtcacattgt acggcgagca tcaggcagcc gcgcaccacc
     2161 gctccgtcga cacgcacgct gcaggcgccg cagacgccgt gctcgcagcc gatatggctg
     2221 cccgtcaatg cagcgtcatc gcgcaggaag tccaccagcg tcttgcgcgc gtcaacggtc
     2281 gcgcgggtct cctcgccatt cacgctcatg acgatgtcgt ggacctgtgc gttcatgacc
     2341 acgcccgatc gagttggcga gcgacgcgcc tgagcagcac gccggcgaga tgggttttca
     2401 ctgcgcccga cgcttgcacg tcgtcgggcg ggttcaggtc ctgcgcaagc gcgcgcaccg
     2461 ccgcgtcgat gtcgccgtcc gccagggccg gctcggcatg gtgtgcgcgg accggcgtgg
     2521 agcctacgcc gaaataggcc aggcgcagat cgaggaggcg cttgccgtac gcgcgcccgc
     2581 tggcggcaag tcccaccatg gcatagtcgc cgtgacggcg cgcgaactca gcgaagccaa
     2641 cacgggtctg ggcgccggct gcgggaatgc ggatggacgt gagcacgtca cgggttgtga
     2701 gcgcggtttc gaacaggcct ttgaagaaat cgtcggcc
//
LOCUS       DATDEU010000134         3211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1920289, whole genome shotgun sequence.
ACCESSION   DATDEU010000134 DATDEU010000000
VERSION     DATDEU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920289"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /gene="uvrC"
                     /locus_tag="VIV63_03335"
     CDS             <1..1538
                     /gene="uvrC"
                     /locus_tag="VIV63_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY6123660.1"
                     /translation="LRDDKSFPYIYLPSGHEYPRLVFYRGARNLPGRYFGPFPGAGAV
                     KETLQSVQKIFRIRNCRDTFFENRSRPCLQHQIGRCSAPCVKLIAREDYARDIAAAVK
                     VLEGRNDEVNGELARAMEQAAESLQYERAAALRDQLAALKQVQSQQIVTAEGDRDIDV
                     FALVGEPGEFAITVMIIRGGRNLGATSYFPKGTLAEPGEALASFLMQYYAETPPPAEV
                     LVNIELGESDALNAALAQRAGTATRVWRPSRALPARWIAMTVENATQALRMRRALRAD
                     IEAMLQDLAGLLGLAAAPRRLECFDISHTAGEGTVASCVVYGPEGPIKKEYRRFNITG
                     VTPGDDYGALRQALRRRYERIKAGEVPAPDVLLIDGGLGQINEVYAELEQLGFGGLKL
                     VGVAKGPDRKPGQERLIVHGDASAIIPGPDSQGLRVVQRIRDEAHRFAITGHRKRRAR
                     RHNESVLETVPGLGPAKRRALLRHFGGMQGLLRAGVNDFEAVSGIGTTLARTLYDHLH
                     PGA"
     gene            1551..2180
                     /locus_tag="VIV63_03340"
     CDS             1551..2180
                     /locus_tag="VIV63_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY6123661.1"
                     /translation="MIWNLPNTLTWLRIFAIPLVIVLFYLPFKWADPAAGLLFALAGI
                     TDSLDGYFARRMGQTSRLGAFLDPVADKLIVAVALVLLVSKDMPMVIVPGLEGIDGGH
                     ATLRSETVRVILVLCSIVIIGREIAISALREWMAEIGQRGKVKVSQLAKYKTILQIVG
                     LSCMLFRWNLWILPVFEIGLVLTIIAAFLTLLSAISYLRAAWPDLRGSE"
     gene            2177..2758
                     /locus_tag="VIV63_03345"
     CDS             2177..2758
                     /locus_tag="VIV63_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY6123662.1"
                     /translation="MIRSVCVFCGSAPGSRPSYSRAAEEMGTALAKAGLTLVYGGGKL
                     GLMGIVADAVLKAGGRVVGVIPRMLIERECAHPNLTSQHVVNTMHERKTLMADMSDAF
                     VGLPGGMGTFDELVEIVTWAQLGLHAKPVILANIENYYAPLYAMLDNAVQEKFVTAES
                     RGRWRNAESVPDVMRILEKEGLLKEGLSRDGSP"
     gene            2801..2876
                     /locus_tag="VIV63_03350"
     tRNA            2801..2876
                     /locus_tag="VIV63_03350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2834..2836,aa:Gly,seq:gcc)
     gene            2907..2980
                     /locus_tag="VIV63_03355"
     tRNA            2907..2980
                     /locus_tag="VIV63_03355"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2939..2941,aa:Cys,seq:gca)
     gene            3000..>3211
                     /locus_tag="VIV63_03360"
     CDS             3000..>3211
                     /locus_tag="VIV63_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123663.1"
                     /translation="MRRWLMSVAVLLTVAAIAGLLVVLANLSRFDEPLLPELEALRTS
                     KSAPLDDNAYPFALGFLAAERRDPRAA"
BASE COUNT          562 a         1021 c         1047 g          581 t
ORIGIN      
        1 tgttgcgcga cgacaagagt tttccgtaca tctatctacc gtcgggccac gagtatccgc
       61 gcctggtgtt ctatcgtggc gcccgcaacc tgccgggccg gtatttcggt ccattcccgg
      121 gcgccggcgc cgtgaaggaa accctgcaga gcgtgcagaa gatcttccgc atccggaact
      181 gccgcgacac cttcttcgag aatcgcagcc ggccgtgcct gcagcaccag atcggccgct
      241 gttcggcgcc ctgcgtgaag ctgatcgcgc gcgaagatta cgcgcgggat atcgccgcgg
      301 cggtcaaggt gctggaaggg cgcaacgatg aggtcaacgg cgaactggcg cgcgccatgg
      361 agcaggccgc cgaatcgctg cagtacgaac gcgcggccgc gttgcgggac caactcgcgg
      421 cgctcaaaca ggtccagtcg cagcagatcg tcaccgccga aggcgatcgc gacatcgacg
      481 tgttcgcgtt ggtgggcgag cccggagaat tcgcgatcac ggtgatgatc atccgcggcg
      541 ggcgcaatct cggcgccacc agctactttc ccaagggcac gctggccgag cccggcgaag
      601 cgctggcgtc cttcctgatg cagtactacg ccgagacgcc accgcccgcg gaagtgctgg
      661 tcaatatcga actgggtgag tccgacgcac tgaacgcggc gctggcgcag cgcgccggca
      721 ccgccacgcg cgtgtggcgg ccgtcacgcg cactgccggc acgctggatc gcgatgacgg
      781 tggagaacgc cacgcaggca ttacgcatgc gccgtgcgct tcgcgcggac atcgaagcca
      841 tgctgcagga cctcgcgggg ttgctaggtc tcgcggcggc cccgcggcgg ctggagtgtt
      901 tcgacatcag ccacacggcg ggtgagggca cggttgcatc ctgcgtcgtg tacgggccgg
      961 agggtccgat caagaaggaa taccggcgct tcaacatcac gggcgtcaca cccggcgacg
     1021 attacggcgc tttgcgccag gcgctgcggc ggcgatatga acgcatcaag gccggcgaag
     1081 tgccggcgcc cgatgtgttg ctgatcgacg gtggactcgg gcagatcaac gaggtctacg
     1141 ccgaactcga acaactggga ttcggcggcc tgaagctggt cggtgtggcg aaagggccgg
     1201 atcgcaagcc cggccaggaa cgcctgatcg tgcatggcga cgcatccgcc atcatccccg
     1261 gtccggattc gcagggatta cgagtggtcc agcgcattcg cgacgaagca caccgcttcg
     1321 ccatcaccgg tcatcgcaag cgacgcgcgc gaaggcacaa cgaatcggtg ctggaaacgg
     1381 tgccgggatt gggacctgcc aagcgccgcg cactgttgcg gcacttcggc ggcatgcagg
     1441 gcttattgcg tgccggtgtc aatgacttcg aggcggtgag cggaatcggg acaacactgg
     1501 cgcgcaccct ttatgatcat ctgcaccctg gagcctgata acttcaccgg atgatctgga
     1561 acctccccaa cacgctgacc tggctgcgga tcttcgcgat cccgctggtc atcgtcctgt
     1621 tctatctacc gttcaagtgg gccgacccgg cggcggggtt gctgttcgcg ctcgccggaa
     1681 tcaccgactc gctggacggc tacttcgcac gccgcatggg acagacatcg cggctcggag
     1741 cgttcctcga tccggtggcg gacaaactca tcgtggcggt cgcactggtg ctgctcgtga
     1801 gcaaggacat gcccatggtc atcgtgcccg ggctcgaagg catcgacggc ggtcacgcca
     1861 cgctgcgttc ggaaacggtg cgcgtgatcc tggtgttgtg ttccatcgtc atcatcggcc
     1921 gcgagatcgc catctccgca cttcgcgagt ggatggcgga aatcggccag cgcggcaagg
     1981 tcaaggtgag ccagctcgcc aagtacaaga ccatcctgca gatcgtcggt ctttcctgca
     2041 tgttgttccg ctggaacctc tggatcctgc cggtgttcga gatcggcctg gtgctcacca
     2101 tcatcgccgc gttcctcacg ctgctctcgg cgatctccta tcttcgggcc gcgtggcccg
     2161 atctgcgagg gtccgaatga tccgttcggt ctgcgtgttc tgcggttccg ccccgggttc
     2221 gcgtcctagc tactcgcgcg ccgccgagga gatgggcacg gcgctggcga aagcggggct
     2281 cacgctggtt tacggcggcg gcaagctcgg gctcatgggc atcgtcgccg acgcggtgtt
     2341 gaaggcgggc gggagggtgg tgggagtgat cccccgcatg ctcatcgaac gcgaatgcgc
     2401 tcacccgaat ctcacgagcc agcacgtggt caacaccatg cacgagcgca agacgctcat
     2461 ggcggacatg tcggacgcct tcgtgggtct gcccgggggc atgggcactt tcgatgaact
     2521 ggtggaaatc gtcacctggg cgcagctcgg gctgcacgcc aaacccgtaa tcctcgccaa
     2581 catcgagaat tactacgcac cgctgtacgc catgctggat aacgccgtgc aggagaagtt
     2641 cgtcaccgcc gaaagccgcg gccgctggcg caatgccgaa tcggtgcccg atgtcatgcg
     2701 aatcctcgaa aaagaggggc tgctaaaaga aggacttagc cgagatggaa gtccttgact
     2761 tgccgggcgg cggccctaga atttgcgccc tctccaggaa gcgggaatag ctcagttggt
     2821 agagcacgac cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccaaatc
     2881 cgcaggtacc ctccgttcgg gcccgtggct aggtggcaga atggttatgc agcggcctgc
     2941 aaagccgtct atgccggttc gattccgacc ctagcctcca tcgagcgact cagtccctga
     3001 tgcgccgttg gctcatgtca gtggcggtgt tgctcacagt cgccgcaatc gcgggactcc
     3061 tcgtggtcct cgccaacctt tcccggttcg acgaacccct gttgcccgag ctcgaggcgc
     3121 tgcgaacttc gaagtccgca ccgcttgacg acaatgctta cccgttcgcg ctgggatttc
     3181 tggccgcgga gcgccgtgat ccacgcgctg c
//
LOCUS       DATDEU010000135         2773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1191147, whole genome shotgun sequence.
ACCESSION   DATDEU010000135 DATDEU010000000
VERSION     DATDEU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191147"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VIV63_03365"
     CDS             <1..1274
                     /locus_tag="VIV63_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123664.1"
                     /translation="LLDDFGDSIELADKVREAHKIWIALVNRTVELEGKARDRDSRVE
                     LLRFQVGELKALDLKEGEVTLLTEERTRHSNRGRLAEAAQAAVGLLYDSDEGNAHATA
                     SRALAGIKTLSSVDQRLAKVIPMVDEATIQIREAARELSRYLETLDIDPARQEEVEKR
                     LAAVEELSRKHRLPSAELAGRASILEKELAELDSADEQLNLLKKSQAESLEAYRKLAT
                     QLSATRQTSARALSKDITARMQTLGMAGGRFLIDVNQANGEPAAHGFDVVEFRVTANP
                     GQPLRPLAKVASGGELSRLSLAVQVACAAEDARCMVFDEVDSGVGGAVAEIVGRELKA
                     LGFSGQAVCVTHLPQVASQGHHHLRVAKLTDGKTTRTTISELTMDERIEELARMMGGK
                     EVTGKAREHAREMLHMAANPDQKVRGGKRKA"
     gene            complement(1311..1760)
                     /gene="fur"
                     /locus_tag="VIV63_03370"
     CDS             complement(1311..1760)
                     /gene="fur"
                     /locus_tag="VIV63_03370"
                     /inference="COORDINATES: protein motif:HMM:NF006999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEY6123665.1"
                     /translation="MESKDLRKAGLKVTGPRLKILEILEGSPTRHMSAEGIYKRLIES
                     NEDIGLATVYRVLTQFQAAGLVSRHHFEDGMAVFELNQGTHHDHIVCLDCGRVEEFID
                     ESIEERQEEIATRLGYELVDHAMTLYGHCMKPGCPSVKRAQKPQKSS"
     gene            1803..2327
                     /gene="bamE"
                     /locus_tag="VIV63_03375"
     CDS             1803..2327
                     /gene="bamE"
                     /locus_tag="VIV63_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY6123666.1"
                     /translation="MKRLALSTISVALGALLASGCVYKMNIQQGNYLVADSVSQLKEG
                     MTRSQVRFLLGTPMVPDAFDENRWDYYYFFSSQKFDEPLKRRLTVFFADDKVARFENL
                     GVPTQADLDQLERDLRKAVAENTKDKKSRGDKTETAAPASESATPSEAEKAPDPVPPS
                     APVPAPAGTPSGPT"
     gene            complement(2548..>2773)
                     /locus_tag="VIV63_03380"
     CDS             complement(2548..>2773)
                     /locus_tag="VIV63_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY6123667.1"
                     /translation="DKRVLMQFVSGPFRVLEGLWTLTPLGDLGCRVELEMRFEFANRV
                     AGALFEPLFEDTAASLVDAFVKRARETKTS"
BASE COUNT          554 a          911 c          837 g          471 t
ORIGIN      
        1 aactgctcga cgatttcggc gacagcatcg agcttgccga caaggtgcgc gaagcccaca
       61 agatctggat cgcgctcgtg aaccgtaccg tcgagctcga aggcaaggcg cgcgaccggg
      121 actcacgcgt ggagttgctc cgcttccagg tcggcgaact caaggcgctc gacctcaagg
      181 aaggcgaagt caccctgctc acggaagaaa gaacgcgcca ttccaatcgc ggccgtctcg
      241 ccgaagccgc ccaggcagca gtcgggcttc tgtacgactc cgatgaaggc aacgcccacg
      301 ccaccgcctc acgcgcattg gccggcatca agactctttc gagcgtcgat cagcggctcg
      361 ccaaggtaat tccgatggtg gacgaagcca ccatccagat tcgcgaagcg gcgcgcgagt
      421 tgtcgcgcta tctcgaaaca ctcgacatcg atccggcccg ccaggaagaa gtcgaaaagc
      481 ggctggccgc ggtcgaagaa ctcagccgca agcaccggct gccgtccgcc gaactcgccg
      541 gccgcgcctc gatcctcgaa aaagaactcg ccgaactcga cagcgccgac gaacaactca
      601 acctgctcaa gaagagccag gccgaatccc tcgaggccta tcgcaagctg gccacgcagt
      661 tgtcggcgac gcgccagact tccgcacgcg cgttgtcgaa ggacatcacc gcgcgcatgc
      721 agacgctcgg aatggctggc ggccgattcc tcatcgacgt caaccaggcg aacggcgaac
      781 ccgcggccca tggtttcgac gtcgtagaat ttcgcgtcac cgcgaatccc ggacaaccgc
      841 tgcgtccact ggccaaggtc gcgtccggtg gcgaattgtc acggttgtcg ttggccgtac
      901 aggtcgcgtg cgctgccgaa gacgcgcgct gcatggtgtt cgacgaagtg gactcgggcg
      961 tgggtggagc cgtggcggaa atcgtcggcc gcgaactcaa ggcactgggc ttctccggtc
     1021 aggctgtctg cgtcactcac ctgccgcagg tcgcgagtca gggccatcac cacctgcgcg
     1081 tggccaaact caccgacggc aagaccaccc gcaccaccat ctccgagctc accatggacg
     1141 agcgcatcga agaactggcg cgaatgatgg gcggcaagga agtcaccggc aaggcacgcg
     1201 agcatgcgcg cgagatgctg cacatggcag ccaatccgga tcagaaggtt cgcggcggaa
     1261 agcgcaaggc ctgaagtccg cgcatcgcgc gacgaccgct cggccgactt ctaactgctt
     1321 ttctggggtt tctgcgcgcg cttgacggac gggcaaccgg gcttcatgca atggccataa
     1381 agcgtcatgg cgtgatcgac gagctcatac cccagacggg tggcgatctc ttcctggcgc
     1441 tcttcgatcg attcatcaat gaactcttcg acgcggccgc agtccagaca cacgatatgg
     1501 tcgtgatgcg ttccctggtt gagctcgaat acggccatgc cgtcttcaaa atgatgccgc
     1561 gaaacgaggc ccgcggcctg gaactgcgtg agcacgcggt acaccgtcgc gagaccgatg
     1621 tcctcgttcg actcgatgag tcgcttgtag atgccttcag ccgacatatg gcgggtcggg
     1681 ctgccctcga gaatttcgag gatcttcagc ctcggccccg tgaccttgag cccggcctta
     1741 cgcaggtctt tgctttccaa gccagcagtc tccgaaaaaa cgatgcggta tgatgcgcca
     1801 ccatgaaacg ccttgcacta tcaaccattt cagtcgccct cggcgcgctt ctcgcctcgg
     1861 gctgtgtcta caagatgaac attcagcagg gcaactatct ggttgcggat tccgtatcgc
     1921 agctgaagga aggcatgacg cgcagccagg tgcgcttcct gctcggcact cccatggtgc
     1981 cggacgcgtt cgatgagaac cgctgggact actactactt cttcagctcg cagaagttcg
     2041 atgaaccgct gaagcgccgc ctgaccgtgt tcttcgcgga cgacaaggtg gcccggttcg
     2101 agaatctggg cgttcccacc caggcggatc tcgaccagct ggaacgcgac ctgcgcaagg
     2161 cggttgcgga gaacaccaag gacaagaaat cgcgcggcga caagacggaa accgctgcac
     2221 cggctagcga gtcggcgact ccttcggagg ccgagaaggc tcccgatcca gtgccgcctt
     2281 ccgcacccgt gcccgctccc gccgggactc cttcgggtcc aacctgagcg gacggtagag
     2341 ctcgatccgg tcgccatcgc gcggcacggt gctgcgctcg cacagttcgc caaagatgcc
     2401 gacgtccgca ttccagggga tgtctacgtc tgctgcctgc tcactcgccc tggcaatcgc
     2461 atccgcaacg gtcgcgtcgt cgggcaatac cactggccac gaccactgcc gcccgggcgt
     2521 cgcgaatgcc acggtgcacc gtttcatcta gctagtcttg gtctcgcggg cgcgcttcac
     2581 gaaagcgtcg accagcgagg ccgccgtgtc ttcgaacagc ggttcgaaca acgcgccagc
     2641 cacccggttg gcgaactcga agcgcatttc gagctcgacg cggcagccga ggtcgccgag
     2701 cggtgtcagc gtccacagtc cttcgaggac gcggaatggc ccactgacga actgcatgag
     2761 gacgcgtttg tcg
//
LOCUS       DATDEU010000136        14870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1045811, whole genome shotgun sequence.
ACCESSION   DATDEU010000136 DATDEU010000000
VERSION     DATDEU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14870
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045811"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1343)
                     /locus_tag="VIV63_03385"
     CDS             complement(201..1343)
                     /locus_tag="VIV63_03385"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY6123668.1"
                     /translation="MDDKARIDQQHSQLCSWLCDAAYPLWSTRGRDPAGGFHERLAQN
                     GEPLIEPRRSRVNPRQAYCFAVAPSLGWRGDSAALVKHALDYFVARYQRPDGLFRTLV
                     AADGSSLDDRALLYDQAFGLLGFNVAAAVGQSRAEREHQAQQLRALVLKTMKRAGPGF
                     DTGVPPSLPLQSNPHMHLFEAALAGCEMCSESSLWKPLADEIADLALSKFIDPGTGAL
                     REFFDESWTPAPGSPGRIVEPGHQFEWAWLLLRWGGAKNTRARTAALKLIDIGEGHGL
                     RNGLATNSMLDDFSAHDAGARLWPQTERLKAAAIAARLTGEARYFSMTVAAADGLLRY
                     LDCPTPGLWYDRIDADGKIVDEPAPASSFYHLVAAIAEISALARLA"
     gene            complement(1357..2637)
                     /locus_tag="VIV63_03390"
     CDS             complement(1357..2637)
                     /locus_tag="VIV63_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEY6123669.1"
                     /translation="MNSNYKGMRAGFATVTTLFFMWGFITVSIDPLIAALKALFDLSY
                     AQAMLTQFAFFMAYFVVSLPAAALIARLGNSKSIMFALGVMVLGCLIVPAATYFETYS
                     LVLVALFVIASGITILQVVANPLAAALGPPERSHFRLTFSQAFNSFGTWLAPRLVSGV
                     ILAGGIFAVTHGAEITPELRAESFRKIDSVFLAIAGALVLLALFIFAFRKLLDVTKPV
                     DEGQRPSVATALKSPWAVYGSAAIFLYVGAEVSIGSAMTNFLHEPDMLNISLEEAGKM
                     VSYYWGGAMVGRLIGSALLFVVKERAPWLLAAFALGAALLCVAVTQLNGSTAAYLALS
                     IGLFNSIMFPVIFTSTLERSTAAPAATSGLLCLAIVGGAILPYVYGLIADSAGLHAAY
                     FLPAVAYLLIVVFAVAATRAKTYQHGTATAAAGH"
     gene            complement(2684..3883)
                     /locus_tag="VIV63_03395"
     CDS             complement(2684..3883)
                     /locus_tag="VIV63_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY6123670.1"
                     /translation="MTERSGKHMRLSGTNLERAGDHNQRVTLHAIRVNGPITRAELAA
                     ITGLTAPAIANITKRLLNENLIQDAGRRRGGRGQPATKLVINPDSCFSIGINIDRDHI
                     TMLVLDFEGRVRARASREVNFAMPKDVQQFFAKGVGKLLTKVGIGRHQLVGVGVALPD
                     DMPKAALPHQPADYGVWSSTDVASLLRETLQLPVFVENDAAAATMGEMQFGLGKKYQT
                     FFYILITAALGGSVVIDGNHFRGASGRSGELGMLRGRDATGHERQIQNIVSLSALYSR
                     LAAQGISVSAPDELTDLDASGQAIIGAWVEVAVDTLLDTVLAINCLMNPEAVLIGGRL
                     PSGIVDQIANRLNQRLEAYAGTVPAISPVARAALSDDAPAVGAAILPFSHRLLPTRSA
                     LMKVTAP"
     gene            complement(3880..4830)
                     /locus_tag="VIV63_03400"
     CDS             complement(3880..4830)
                     /locus_tag="VIV63_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY6123671.1"
                     /translation="MSPATKLLAGVELGGTKCVCILGTGPDDVRAVERLPTGEREETL
                     RQIEAVLDRWRDQYGAARALGIASFGPVDLRTESPTYGYITSTNKPGWRNTDVAQRLG
                     RRAGVPMVFDTDVNGAALAEGRWGAAAGLDDFAYVTVGTGIGVGLIVRGKSIFGMNHT
                     ELGHIRVVRKPGDEFAGVCSFHGDCIEGLASGPAIEARAGMPASQLPPDHPAWDFVAH
                     GLAQLIHTMVLTTAPSRIFLGGGVMSAQTHLFERIQQELKRSLNRYVETPELEQGLAH
                     FIVPPGLGTMAGPLGALALAADAEGAATATKTPAITAASR"
     gene            complement(4830..6368)
                     /locus_tag="VIV63_03405"
     CDS             complement(4830..6368)
                     /locus_tag="VIV63_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6123672.1"
                     /translation="MEPDRRIRRVAIVGGGIAGWTAAVMLGKKLGGQCSIHVVEAPEA
                     VYPGLAEATQPTILELLRYLGIDQNDFIDKTQSTYSLGTRFTDWAAPGENFWHPFGSL
                     GALIERRPFYHFWHKGKALGLKPRLELFSHEVSMAMTNRFIFPTNSLGVAPHLRYALH
                     VDSGLTARYLRTIAERAGVILLARKVVSATRREDGFIDELQFEDGGKLRADLYIDCSG
                     SRAQLIGEILETPYDSWQKWLPCDRLVHAPSALEEVRPPFVRVRAREAGWQWRIPLQQ
                     NLSHALVYSSAHQSDESAMAELVGDLGATSPAVPRRQDFIQGRRRKFWDKNVVSLGTA
                     SSFLEPLVAADLHLTTNALFNLLDHFPDKQFDPANIASYNAQIGDELERIRDFTILHY
                     NLSRRDVSPFWQQSGKVDLPDALAQRIDLYRASGKIIQQRPELFSDVDWFWIFEGSGV
                     TPRDYDPLVDTVDFEQVKRLMLAISQKVTADSAAAPTHDSFFAQANSKLASARKAAAA
                     QTAG"
     gene            6631..9090
                     /locus_tag="VIV63_03410"
     CDS             6631..9090
                     /locus_tag="VIV63_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo 1,3/1,4-beta-D-glucan glucohydrolase"
                     /protein_id="HEY6123673.1"
                     /translation="MAVAEDPAAVHPEIWPSPKWPFEKDAALEEKVAALLKKMTVEEK
                     VGQVIQGDICCIKPEDMKKYHLGSILAGGGSSPGNNERSPAKDWLKLADEFYAASVDT
                     SNGGVGIPMIWGIDAMHGHSNIVGAVLFPHNVGLGATHNPKLLGDIARVTAEQVRTTG
                     IEWTFAPTVTVPQDDRWGRAYEGYSEDPKLVASYAGEFVKGLQGDPVSPDFLKGAHVI
                     SSTKHYLADGGTENGRDQGDSKASETELRDIHNAGYVPAIEAGVQTIMISFSSWQGQK
                     LSGHKGLITDVLKKRMNFDGFTVGDWNGHGQVAGCTNDNCAAAFNAGMDMFMAPDSWK
                     GLYENTLKQAKDGTISMERLDEAVTRILRVKFRAGVFDGAAPSKRPLGGDFKMLASPE
                     QRAIARDAVRQSLVLLKNNGGLLPLAANKHVLVTGDGADNISKQNGGWTITWQGTGLT
                     NADFPGATSVWGGVKAAVKAGGGSAELSADGSFKKKPDYAIVVFGENPYAEFQGDLKV
                     QTLPGSMTQQLEIMKKLQDEKIPVVAILFSGRPLWQNRELNLSQAYVAAWLPGTEGGG
                     ISDVLFRKKDGSVNYDFTGRLSFSWPRDATGTPLNVNKDPYDPLFPFGFGLSYTAAAD
                     LGPLPEDPGIPAELMSTGSFFDKGVPVQPWSLRVSSGSDTTRITTVPTDAVGGRVKVT
                     AVDDAVQEGGRRFQFAGDGPATIAITSEGAVDVSRESNGDVMLLVRLRRDADVPKDVT
                     LGVSCGAGCGGKVPFADALGGVASGKWQTVGVSLKCFVKAGADVSKLNETLSIESSGK
                     LDVSISQVKLGTVADKVVSCN"
     gene            complement(9238..9504)
                     /locus_tag="VIV63_03415"
     CDS             complement(9238..9504)
                     /locus_tag="VIV63_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HEY6123674.1"
                     /translation="MATGWAQDGAVQDQIDATVKDGIKRATSRLPSGPSLESCAECGA
                     KIPEARQQAIPGVRLCVTCQEKEDREIVNNAGYNRRGSKDSQLR"
     gene            complement(9577..10314)
                     /locus_tag="VIV63_03420"
     CDS             complement(9577..10314)
                     /locus_tag="VIV63_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY6123675.1"
                     /translation="MSQFSRRSLIAGSIGAGLALPALALADEQCGERPDTAPALQPPL
                     LLTDWAWLARYRDDNAKLLASGMKVDTVFLGDSITEGWARADTGFFSKGIVGRGISAQ
                     TTPQLLVRMHADVIALKPRAVHIMAGTNDIAHNTGPMTAQDSQNNLMAMCEIAKAHKL
                     RVVLGAVPPASKYWWRPEAQPKPEALALNEWMRGYAKQIGAKYADYAAVLTDANGDVK
                     PELAKDQVHPSPAGYAAMRKVAEAAIR"
     gene            complement(10311..11672)
                     /locus_tag="VIV63_03425"
     CDS             complement(10311..11672)
                     /locus_tag="VIV63_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY6123676.1"
                     /translation="MSESRSLAARNLSVIVTASLAGLLFGFDTVVMSGVTQSLRDVFH
                     LAPGSWWFGFAVASALFGTTLGAMFAGIPGDAFGSRDSLKAVGVLYVVSALGSALCWN
                     LPSFYVFRFIGGLAIGASSVLAPVYISEIAPANRRGALTGLFQFNIVFGILLAYVSNF
                     AVQWIAGDVDAWRWKLGVAAVPAFIFLLLMYTIPQSPRWLSLRGREGEAKASLERLGV
                     SDPAGMLAEFARAANEARSAAAQVLFSSRYSRPIVLAILLAMFNQLSGINAILYYLND
                     IFAAAGFDKMSSDLQAIAIGAANLIATMIALRVIDKFGRRKLLLIGAIGTAVAQAGVA
                     LIMGSGQGNEYLLAMLIMFIAFFAFSQGAVIWVYLAEIFPTAVRSRGQALGSTTHWLM
                     NALIATVFPMIAATAQGLPFAFFAVCMVVQFFVVLALFPETRGVELESMDKTIEKKTG
                     ASA"
     gene            11759..>14870
                     /locus_tag="VIV63_03430"
     CDS             11759..>14870
                     /locus_tag="VIV63_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY6123677.1"
                     /translation="MSKRSRAQVCASLTILLTLFSSAAPAQSPLAPLSGPFNVTILEG
                     GEGLTRDLDSGAVSLAANEPWAMSGWVNPQRIQPGGSVLFAVGGSQAEGRALILREGK
                     PALWAGGKDLSSDTAIVPGAWTFLAASHDGNRARLYVNGREVATHEAQIGAAAPKIHV
                     APVIQHIRDVPHFGGRLAGLSLHNEMLTAKQVRALYTARPNFDVVVFHEVGGHWPWQV
                     KQWRGLQDPQDPWTLPKATTPFSKPVAKPLTLEPALAPAGEGTWALRRWRLAEAPKVD
                     AKPAEISDANYSDTAWYAATVPGTVLTTLIDRGVYPDYDYGLNNMAIPESLARQDYWY
                     RTVFDAPASFDGQQLTLTFKGINYAAEVWLNGGRLGNIRGAFIRGVFDVTGRVAPGKR
                     NVIAVRVSPPPHPGIPHEESIAAGPGENGGWVALDGPTFAAAEGWDWIPGIRDRNTGI
                     WQDVELKASGRVRLLDPQVITRLNLPKNDSADVSILVAVDSSHIAPVQVTIDVSFEGV
                     KLTKSTSVSPGKSEVRFTAAEFPALTVKNPRLWWPNGYGEPALYHLALAVADRGRPSD
                     TKSLRFGIRHVTYEFSLFDSAGRLRRVEVDVAGGSARGERLIDIRHEAIKKTPQGWAA
                     SLTKVGEASPAVRELDDPLQFPHLTLRVNGVRILARGGNWGMDDSRKRIQRERLEPYF
                     RLQKDANLNIIRNWMGTNTQDSFYDLCDEYGMMVLNDFWASTQDFQVEPQDPQLFLQN
                     AEDTVQRYRNHPSIVLWFGRNEGVPQPILNEGLGDVVAKYDGTRHFTGSSNNVNLQGS
                     GPYNYRPPVGYFTDLACGFSVETGTPSLATLEAIEAMVPKADRWPLSDTIAYHDWHFG
                     GNGDIKTFMATLATRYGAGTSLEDFERKAQMMNYDTYRAMFEGLQAHLWTKNSGRLLW
                     MTHPAWPSNHWQIYSHDYDTHAAYFGVKKAVEPVHVQMNLPDFSVTVVNSTREARDGL
                     KLELRVFKLDGVQLFDHLHPVDARANDITPLGDLGIAERVAKEGMVLVALRLRDRTGA
                     LVSDNTY"
BASE COUNT         2795 a         4798 c         4725 g         2552 t
ORIGIN      
        1 cgacatgaaa gcccccacta acaatgtttc acgcgaccgc gacgaatgtc gcaacacgca
       61 tgagtcgatc ccaagtggtg gcgatcttgc agcgccccat ttcgccttgt caataaatac
      121 ttacatttgc tttcattaaa aaaacgacaa ttggtgcgac gcggcaatcg gtaccgctaa
      181 caacaatcag cgagggagtt tcaggcgagg cgcgcgagcg cgctgatttc cgcgatggca
      241 gcgacgagat ggtagaagct gctcgccggc gcaggctcgt cgacgatttt tccatccgca
      301 tcgatgcggt cgtaccagag tccgggagtg ggacagtcga gataacgcag caggccatcc
      361 gcggccgcca cggtcatcga gaaatagcgc gcctcgccag tcagacgcgc ggcgattgcc
      421 gcggccttga gcctttcggt ctgcggccac agtcgggcgc cagcatcgtg cgccgagaaa
      481 tcgtcgagca tgctgttggt cgcgagacca ttgcgcaggc catgaccttc accgatgtcg
      541 atgagcttga gcgccgcggt acgtgcgcgc gtgttcttcg ccccacccca tcgcaacagc
      601 agccatgccc attcgaactg gtgtccaggc tcgacgatgc gtccgggact tcccggcgcg
      661 ggggtccagc tctcgtcgaa gaactcccgc aacgcgccgg tgccgggatc gatgaacttc
      721 gacaacgcca ggtcggcgat ctcgtcggcc aacggcttcc acaacgagct ctccgaacac
      781 atctcgcatc ccgcaagcgc cgcttcgaac agatgcatgt gcgggttcga ctgcagcggc
      841 aggctcggcg gcacaccggt atcgaatccg ggccctgccc gtttcatggt cttgagcacc
      901 agcgcgcgca actgctgcgc ttgatgctcg cgctccgcgc gcgactgacc gaccgccgcc
      961 gccacgttga acccgagcag accgaaggcc tggtcgtaca ggagtgcgcg gtcatcgagc
     1021 gacgaaccgt cggccgcgac cagcgtgcgg aacaacccgt cgggtcgctg atatctcgcg
     1081 acgaagtaat cgagcgcgtg cttcaccagc gccgcggagt cgccgcgcca gccgagagac
     1141 ggcgccaccg cgaaacagta cgcctgacgt ggattgacgc gcgagcgccg cggctcgatc
     1201 agcggctcgc cgttctgcgc gagccgctcg tgaaagccac cggccggatc gcggccacgc
     1261 gtcgaccaga gtggataggc ggcatcacac agccagctgc agagctggct gtgttgttga
     1321 tcgatgcgcg ccttgtcgtc cataactctc ctggcttcaa tgacccgcgg cagcggtcgc
     1381 cgtgccgtgc tggtaggtct tcgcgcgggt tgcggccacc gcgaacacca cgatcagcag
     1441 ataggcgacg gccggcagga aatatgcggc atgcagcccg gccgaatccg cgatcaggcc
     1501 gtagacatac ggcagaatcg cgccgccgac aatggcaagg cacagaagcc ctgacgtcgc
     1561 cgcgggagcg gcggttgagc gttccagcgt cgacgtgaag atcacgggga acatgattga
     1621 attgaaaagg ccgatcgaca gcgcgagata cgctgcggtc gaaccgttca attgcgtcac
     1681 ggcgacacaa agcagcgcgg cgccgagcgc gaaggccgcg agcagccagg gcgcgcgttc
     1741 cttcacgacg aacagcaatg cgcttccgat caatcggccg accatcgccc caccccagta
     1801 gtagctcacc atttttcccg cttcttccag cgaaatattg agcatgtcgg gctcgtgcag
     1861 gaagttggtc atggcgctgc cgatcgatac ttcggcgccg acgtacagga aaatcgctgc
     1921 ggatccgtac accgcccacg gcgacttcag cgccgttgcc accgaggggc gctggccttc
     1981 gtcgaccggt ttcgtcacat ccagcagctt gcggaacgcg aagatgaaca gcgcaagcaa
     2041 aacgagtgct ccggcgatgg ccaggaaaac actgtcgatc ttccggaaag attcggcgcg
     2101 cagctccggc gtgatttcgg cgccgtgtgt cacggcgaag atgccgccag cgagaatcac
     2161 gcccgagacg agccgtggcg cgagccaggt gccgaacgaa ttgaaagcct gcgagaacgt
     2221 gagtcggaaa tgcgagcgct caggcgggcc tagcgctgcg gccagcggat tcgcgactac
     2281 ctgcaggatc gtgatgccgc tcgcgatgac gaacaacgcc acgagcacca acgaataggt
     2341 ctcgaagtac gtggccgccg ggacgatgag gcacccgagg accatgacgc ccagcgcgaa
     2401 catgatcgac ttgctgtttc cgagccgcgc aatcaacgcg gcagccggca aggagacgac
     2461 gaagtacgcc atgaagaatg caaactgcgt gagcatcgcc tgcgcgtaac tgagatcgaa
     2521 aagcgctttg agcgccgcga tgagcgggtc gatggaaacg gtgatgaagc cccacatgaa
     2581 gaacagtgtg gtcaccgtcg cgaaacccgc acgcatgcct ttgtagttag agttcatcgg
     2641 tcccctcgga agattgagcg aaagttctcg aacaggaaaa tttctagggc gccgtcactt
     2701 tcatgagcgc ggaacgcgtc ggcagcagcc gatggctgaa cggcaggatg gccgcgccga
     2761 cggccggtgc gtcatcggac aacgcggcgc gggccacggg cgagatcgcc ggtaccgtgc
     2821 ctgcataagc ctcgaggcgc tggttcagcc ggttcgcaat ctggtcgacg atgccggaag
     2881 gcagccggcc accgatgagt accgcctcgg gattcatcag gcagttgatg gccagcaccg
     2941 tgtcgagcag tgtgtcgacc gcgacttcca cccacgcgcc gatgatggcc tggccgctgg
     3001 catcgagatc ggtgagttcg tccggagccg agacgctgat gccttgcgcc gcgagccggc
     3061 tgtacagcgc cgacagcgac acgatgttct ggatctggcg ttcgtggccc gtggcgtcgc
     3121 gcccgcgcag catgccgagc tcgccactgc ggccactggc accgcgaaaa tgattgccgt
     3181 cgatcaccac gctgccgccg agtgcggccg tgatgagaat gtagaagaac gtctggtatt
     3241 tcttgcccag cccgaactgc atctcgccca tggtggcggc ggccgcgtcg ttctcgacga
     3301 acacgggtag ttgcagcgtc tcacgcaata gcgatgcgac atcggtcgat gaccagacgc
     3361 cgtagtcggc cggctgatgc ggcagcgccg ctttcggcat gtcgtcgggc agcgccacgc
     3421 cgacgcccac cagctggtgg cggccgatgc ccaccttggt cagtagtttg cccacgccct
     3481 tggcgaagaa ctgctgcacg tccttgggca tcgcgaaatt cacttcgcgt gaagcgcggg
     3541 cgcgaacccg gccctcgaaa tcgagcacca gcatcgtgat gtgatcgcga tcgatgttga
     3601 tgccgatcga aaagcagcta tccgggttga tgaccagctt cgtcgcgggc tggccacggc
     3661 cgccccggcg tcggcccgca tcctggatca ggttttcgtt gagcagccgc ttggtgatgt
     3721 tggcgatggc cggcgcggtg aggccggtga tcgcggcaag ctctgcgcgc gtgatcggcc
     3781 cgttgacgcg gatcgcgtgc aacgtgacgc gctgattgtg atcgcccgcg cgttcgaggt
     3841 tggtccccga caatcgcatg tgcttgccgg agcgttcggt catcgcgaag cggcggtgat
     3901 cgccggcgtt ttggtcgcag tggccgcgcc ttcggcatcc gccgcgagcg cgagcgcgcc
     3961 gagaggtccg gccatggtcc cgagtccggg aggcacgatg aaatgcgcga gaccttgttc
     4021 gagttccggc gtctcgacgt agcgattgag gctgcgcttg agttcctgct gaatgcgctc
     4081 gaacaggtgt gtctgggcac tcatcacgcc gccgccgagg aagatgcgcg acggcgcggt
     4141 ggtgagcacc atggtgtgaa tcagctgcgc gagcccatgc gccacgaagt cccaggcggg
     4201 atggtccggt ggcaattgcg atgccggcat accggcccgc gcctcgatgg ccggacccga
     4261 cgccaggcct tcgatgcaat cgccgtggaa cgaacacacg ccggcgaact cgtcgcccgg
     4321 cttgcgcacg acgcggatgt ggccgagctc ggtgtggttc atgccgaaga tcgatttgcc
     4381 gcgaacgatc aggcccacgc caatgccggt gcccaccgtg acgtaagcga aatcgtcgag
     4441 gcctgccgcg gcgccccagc gcccttcggc gagcgcggcg ccattcacgt cggtgtcgaa
     4501 aaccatcggc acgccggcgc gccgccccag ccgctgcgcc acgtctgtat tgcgccagcc
     4561 cggtttgttg gtcgaggtga tgtagccgta ggtcggggat tcggtacgaa gatcgacggg
     4621 accaaacgac gcgatcccta aggcgcgcgc ggcgccgtat tgatcacgcc agcggtcgag
     4681 caccgcctcg atctgccgca acgtctcttc acgctcgccc gtcggcaggc gctcgacggc
     4741 gcgcacgtcg tccgggccgg tgccgagaat gcacacgcat ttcgttccgc ccagttcgac
     4801 gccggcaagt agtttggtgg cgggcgacat cagccagccg tctgggccgc ggcggccttg
     4861 cgcgcactgg cgagcttcga gttcgcctgc gcgaagaagc tgtcatgcgt cggcgcggcc
     4921 gcggaatccg cagtgacctt ctggctgatg gccagcatca ggcgtttgac ctgttcgaag
     4981 tccacggtat cgaccagcgg atcgtagtcc cgcggcgtaa cgcccgagcc ttcgaagatc
     5041 cagaaccagt cgacgtcgga gaacagctcc gggcgctgct ggatgatctt gccggaggcg
     5101 cgatacagat cgatgcgttg cgcaagcgcg tccggcagat cgaccttccc gctttgctgc
     5161 cagaacggcg agacgtcgcg acgcgaaagg ttgtaatgca ggatggtgaa gtcacggatg
     5221 cgttccagct cgtcgccaat ctgggcgttg tagctcgcaa tattggcggg gtcaaactgc
     5281 ttgtccggaa agtggtcgag cagattgaac aatgcgttgg tagtcaggtg cagatcggcg
     5341 gcgaccagcg gctcgagaaa gctcgaagcg gttccgagcg acaccacgtt cttgtcccag
     5401 aatttgcggc ggcgaccctg gatgaaatcc tggcggcggg gcaccgcggg cgacgtggcg
     5461 cccagatccc cgaccagttc cgccatcgcg gattcatcgc tctggtgagc gctcgaatac
     5521 accagcgcgt gactcaggtt ctgctgcagc ggtatgcgcc actgccagcc ggcttcgcgc
     5581 gcgcgtacgc gcacgaacgg cggacgaact tcttcgagcg ctgacggcgc atgcaccagc
     5641 cggtcgcagg gcagccactt ctgccagctg tcgtaaggcg tctcgagaat ttcgccgatc
     5701 agttgcgcgc gcgaaccgct gcagtcgatg tagagatcgg cgcgcagctt gccgccgtcc
     5761 tcgaattgca gttcgtcgat gaagccgtct tcgcggcgcg tcgcgctaac aaccttgcgc
     5821 gccagcagga tgacgccggc acgctcggcg atcgtgcgca ggtaacgggc cgtgagcccc
     5881 gagtcgacgt gcagcgcgta acggaggtgc ggcgcgacac ccagcgagtt ggtcgggaag
     5941 atgaagcgat tggtcatcgc catggacact tcgtggctga agagctccag ccgcggcttg
     6001 agccccagtg ccttgccctt gtgccagaag tggtagaacg gccggcgctc gatgagtgcc
     6061 cccagcgagc cgaagggatg ccagaagttc tcgcccggag ccgcccagtc ggtgaaccgg
     6121 gtgccgaggc tgtaggtcga ctgcgtcttg tcgatgaagt cgttctggtc gatccccagg
     6181 tatcgaagca gttcgaggat ggtgggctga gtcgcctcgg ccagtcccgg atacacggcc
     6241 tcgggagctt cgacgacgtg aattgaacat tgtccgccga gctttttgcc cagcatgacg
     6301 gccgcggtcc acccagcgat accaccgcca acgatggcaa cccgcctgat tcttcgatcc
     6361 ggttccattt acttctttag acctatccgc cccaacgcgg cggcgaggct atccgtgcga
     6421 cccgaaacct gtcaacgaat cgccacttag cgcgcccaac cctcgaattt ccgccgcaga
     6481 gagcataggc tgtccgcgct caaaaacagg agtttttcga tgtccccttc catgtctcat
     6541 gtccgtaatc atggcgcgac actgttggtg ctcgctcctt tcctgttgat gcttggatgc
     6601 aaagaagcgg ccaaggaaga acccgccccc gtggccgtag ccgaagatcc ggcggcggtc
     6661 catcccgaaa tctggccctc tccgaagtgg cccttcgaga aagacgcggc actcgaagaa
     6721 aaggtagcgg cgctgctcaa gaagatgacg gtcgaggaaa aagtcggcca ggtcatccag
     6781 ggcgacatct gctgcatcaa gcccgaggac atgaagaaat accatctggg ctcgatcctt
     6841 gccggcggcg gttcctcgcc tggcaacaac gagcgctcgc cggcaaagga ctggctcaag
     6901 ctcgccgacg agttctacgc cgcgtccgtc gacaccagca atggcggcgt cggcatcccg
     6961 atgatctggg gcatcgatgc gatgcacggc cacagcaaca tcgtgggcgc cgtgctgttc
     7021 ccgcataacg tgggcctcgg cgccacgcac aatcccaagt tgttaggcga catcgcgcgg
     7081 gtgaccgccg agcaggtgcg caccaccgga atcgaatgga cctttgcgcc cacggtgacg
     7141 gtgccgcagg acgatcgctg gggccgtgcc tacgagggct actccgaaga cccgaaactc
     7201 gtggcgtcct atgccggcga gttcgtgaag ggtctgcaag gcgatcccgt ctcgcccgac
     7261 ttccttaagg gcgcgcacgt gatctcctcc accaagcact acctcgccga cggtggcacc
     7321 gagaatggcc gcgaccaggg cgactcgaag gcttcggaga ccgagctgcg cgacatccac
     7381 aacgcgggct acgtaccggc catcgaagcg ggcgtacaga ccatcatgat ctccttctcg
     7441 agctggcagg gtcagaagct ttcgggccac aagggcctga tcaccgacgt gctcaagaag
     7501 cgcatgaact tcgacggctt caccgtcggc gactggaatg gtcacggcca ggtggccggc
     7561 tgcaccaacg acaactgcgc cgcggccttc aacgccggca tggacatgtt catggcgccg
     7621 gatagctgga agggcctgta cgagaacacg ctcaagcagg cgaaggacgg aaccatctcg
     7681 atggaacgtc tggacgaggc cgtgacgcgc attcttcgcg tgaagttccg cgccggtgtg
     7741 ttcgatggcg ccgcgccgtc gaagcgtccg ctgggtggcg acttcaagat gctggcatcg
     7801 cccgaacagc gcgccatcgc gcgcgacgcc gtgcgccagt cactggtact gctcaagaac
     7861 aacggcggac ttctgccgct cgcggccaac aaacatgtgc tggtcaccgg cgacggcgcc
     7921 gacaacatca gcaaacagaa tggcggctgg accatcacct ggcagggcac cggcctcacc
     7981 aacgccgact tccccggcgc gacctcggtg tggggcggcg tgaaggccgc ggtgaaagcc
     8041 ggcggaggtt cggccgaact gtccgccgat ggcagcttca agaagaagcc tgactacgcc
     8101 atcgtcgtgt tcggtgagaa tccttacgcc gagttccagg gcgacctcaa ggtgcagaca
     8161 ctgcccggct cgatgactca gcaactcgag atcatgaaga agctgcagga cgagaaaatt
     8221 cccgtcgtgg ccatcctgtt ctcgggccgg cccttgtggc agaaccgcga gctgaatctt
     8281 agccaggcgt acgtcgcggc ctggctgccg ggtaccgaag gcggcggcat cagcgatgtg
     8341 ctgttccgca agaaggatgg ttcggtgaac tacgacttca ccggcaggct gtcgttttcc
     8401 tggccgcgtg atgccacggg cacgccgctc aacgtgaaca aggatcccta cgatccgctg
     8461 tttccgttcg gcttcgggct cagctacacg gcggctgctg acctggggcc gctgcctgaa
     8521 gatccgggca ttcccgcgga gctcatgtcc acgggttcgt tcttcgacaa gggcgtgccc
     8581 gtgcagccct ggtcgttgcg cgtttcgagc ggcagcgaca ccacgcgcat caccaccgta
     8641 cctaccgacg ccgtcggtgg ccgcgtgaag gtcaccgcgg tggacgacgc cgtgcaggaa
     8701 ggcggtcgcc gcttccagtt cgcaggtgac ggtcccgcga ccatcgcgat caccagcgag
     8761 ggcgcggtcg acgtgtctcg cgaatcgaac ggcgacgtca tgttgctggt gcgcctgcgt
     8821 cgcgatgccg acgtacccaa ggacgtgaca ctcggcgtga gctgtggcgc gggttgcggc
     8881 ggcaaggtgc cgttcgcgga cgcgctgggt ggcgtcgcga gtggcaagtg gcaaaccgtt
     8941 ggcgtgtcgc tcaagtgctt cgtcaaggcc ggcgcggacg tgagcaagct caacgagacg
     9001 ctcagcatcg agtcgagcgg caagctcgac gtctccatct cgcaggtcaa gctcggcacc
     9061 gtggccgaca aggtagttag ctgcaattaa tggggtcaga ccccatttcc aggtaatggt
     9121 gccggacctg ccggcagacg taaggggcgc tcattcgagc gccccttttt atttcgtcag
     9181 cgatgaatgc caacttgagg tccggcacaa tttgccagga aatggggtct gaccccatta
     9241 acgcagctgg ctgtccttgc tgccacgccg gttgtagccg gcgttgttca cgatctcgcg
     9301 gtcttccttt tcctggcagg tcacgcagag gcgaacgccg ggaatcgcct gctgtcgcgc
     9361 ctcgggaatc ttcgcgccgc attccgcgca cgactcgagc gacggccctg acggcaggcg
     9421 gctcgtcgcc cgcttgatgc cgtctttcac cgtcgcatcg atctgatcct ggactgcgcc
     9481 gtcctgtgcc caaccggttg ccatggcgct atttatggcg gcccatgggt ctattgcaag
     9541 cacaggccgg caccggcgtt ttcctacccc gacctgctac cgaatcgccg cctcggccac
     9601 cttccgcatc gccgcgtaac ccgcggggga cggatgcacc tggtccttcg ccagctccgg
     9661 cttcacgtcg ccattcgcat cggtgagcac cgcggcatag tccgcgtact tcgcgccgat
     9721 ctgcttcgcg taaccgcgca tccactcgtt cagcgcgagc gcttccggtt tgggctgcgc
     9781 ctcgggcctc caccagtatt tcgacgccgg ggggaccgcc ccgagcacca cgcgcagctt
     9841 gtgcgccttc gcgatctcgc acatcgccat gaggttgttc tgcgagtcct gcgccgtcat
     9901 cggccccgtg ttgtgggcga tgtcgttggt gcccgccatg atgtgcacgg cgcgcggctt
     9961 gagcgcgatg acatccgcat gcatgcgcac cagcagctgc ggcgtggtct gcgcgctgat
    10021 gccgcgcccc acgatgcctt tcgagaagaa cccggtatcc gcccgcgccc aaccttcggt
    10081 gatcgagtcg ccgagaaaca ccgtgtcgac tttcatgcca ctcgcaagca gtttggcgtt
    10141 gtcatcgcga taccgcgcca gccacgccca gtcggtgagc aacagcggcg gctgcaacgc
    10201 gggcgcggtg tcaggccgtt cgccgcattg ctcgtccgct agcgccaatg cagggagtgc
    10261 gagccctgcg ccgatggaac ctgcgatcaa cgatcgacga ctgaactgac tcatgcgctt
    10321 gctcctgttt tcttttcgat ggtcttgtcc atcgactcca gttccacgcc gcgcgtctcc
    10381 ggaaacaacg cgagcaccac gaagaactgc accaccatgc agaccgcgaa gaatgcgaac
    10441 ggcaggccct gcgccgttgc cgcgatcatc gggaacaccg tggcgatgag cgcgttcatg
    10501 agccagtgag tggtgctgcc cagcgcctgg ccgcgggagc gcaccgcggt cggaaagatc
    10561 tcggcgagat acacccagat caccgcgccc tgcgagaacg caaagaacgc gatgaacatg
    10621 atcagcatcg ccagcaggta ttcgttgccc tgcccgctgc ccatgatcag cgccacgccc
    10681 gcctgcgcca cggccgtgcc gatcgcgccg atcagcagca gcttcctgcg gccgaacttg
    10741 tcgatgacgc gcagcgcgat catggtggcg atgaggttgg cggcgccgat ggcgatggcc
    10801 tgcaggtccg acgacatctt gtcgaagccc gcggcggcga agatgtcgtt gaggtagtac
    10861 aggatggcgt tgatgccgga gagctggttg aacatcgcca gcagtatcgc gagcacgatc
    10921 ggccggctgt accgtgagga aaacagcacc tgcgcggcag ccgagcgcgc ttcgttcgcg
    10981 gcgcgcgcga actcggcgag catgcctgcc ggatcgctca cacccagcct ctccaggctc
    11041 gccttcgctt cgccttcacg gcctcgcagg ctgagccagc gcggactctg cggaatcgtg
    11101 tacatcaaca gcaggaagat gaaagccggc acggcggcca cgcccagttt ccatcgccag
    11161 gcgtccacgt caccggctat ccactgcacc gcgaagttgc tcacgtaggc gagcaggatt
    11221 ccaaacacga tgttgaactg gaacagtccg gtaagcgcgc cgcgacggtt tgccggcgcg
    11281 atctccgata tgtacaccgg cgccagcacc gatgaagcgc cgatcgcgag gccgccgatg
    11341 aaacggaaca cgtaaaacga cggcaggttc cagcacaacg cgctgcctaa cgccgacacc
    11401 acgtacaaca cgcccacggc tttcagggaa tctcgcgagc cgaaagcatc gccgggaatt
    11461 cccgcgaaca tggctccgag cgtggtgccg aacagcgcgc tcgccacggc gaagccaaac
    11521 caccagcttc cgggtgccag gtgaaagaca tcgcgcagcg actgcgtgac gcccgacatg
    11581 accaccgtat cgaagccgaa caacaggccc gcgaggctcg cggtgacgat tacgctgaga
    11641 ttcctcgcgg caaggcttct ggattccgac atcgtccccc cgacgtatat aagaagagag
    11701 cagctattaa tgaaagtcgt tttggttata gtaaggcatc ggtcacgggg gtgggaacat
    11761 gtcgaagcgg tctcgcgcgc aggtgtgcgc gtctctaaca atcttgctca cgctgttttc
    11821 ctcggccgcg ccggcgcaat cgccactcgc cccactctcc ggtcccttca acgtcaccat
    11881 actcgaaggt ggcgaaggcc tcacgcgtga tctcgacagc ggcgccgtgt cgctggccgc
    11941 gaacgaaccg tgggcgatga gcggctgggt gaatccccaa cggattcagc cgggcggctc
    12001 cgtgttgttc gcggtgggcg gatcgcaggc cgaaggccgc gcgctgattc ttcgcgaggg
    12061 caaacccgcg ttgtgggcag gaggcaagga cctttcgagt gatacggcga tcgtacccgg
    12121 cgcctggaca ttcctcgccg ccagccatga cggaaatcgc gcgcggctct atgtgaacgg
    12181 tcgggaggtc gccacgcacg aagcacagat cggcgctgcg gctccgaaga tccacgtcgc
    12241 gcccgtgatc cagcacattc gcgatgtacc ccacttcggc ggccggctcg cgggactgtc
    12301 gttgcacaac gaaatgctga cggccaaaca agttcgcgcg ctgtacaccg cgcgccccaa
    12361 cttcgatgtc gtcgtattcc acgaagtcgg cggccactgg ccctggcagg tcaagcaatg
    12421 gcgcggcctg caggacccgc aggatccgtg gacgctgccg aaagcgacga cgcccttcag
    12481 caaacccgtc gcgaagccgc tgacgctcga gcctgcgctc gcgcctgccg gcgaaggcac
    12541 ctgggcactg cggcgctggc gcctcgccga agcgcccaag gtggacgcga aacccgccga
    12601 gatctccgac gctaactact ccgataccgc gtggtacgcg gccaccgttc ccggcaccgt
    12661 actcaccacg ctcatcgatc gtggcgtgta cccggactac gactacggcc tcaacaacat
    12721 ggcgatcccg gagagcctgg cgcgccagga ctactggtat cgcacggtat tcgacgcacc
    12781 cgcatcgttc gatggccagc agctcacgct cacgttcaag ggcatcaact acgccgccga
    12841 ggtgtggctc aacggcggcc ggctcggcaa catccgcggc gcattcattc gcggcgtatt
    12901 cgatgtcacc gggcgtgtcg cgcccggcaa gcgcaacgtg atcgcggtgc gcgtgtcgcc
    12961 gcctccgcat cccggcattc ctcacgagga atccatcgcg gccggccccg gcgaaaacgg
    13021 cggctgggtc gcgctcgatg gccccacgtt cgcggcggca gaaggctggg actggattcc
    13081 cggcatccgc gaccgcaata cgggtatctg gcaggacgtc gagctcaagg cgtccggccg
    13141 cgtgcggtta ctggaccccc aggtgatcac gcgcctgaat ttgccgaaga acgacagcgc
    13201 cgatgtgtcc atcctcgttg cggtagatag ttcgcacatc gcgccagtac aggtgaccat
    13261 cgacgtcagc ttcgaaggcg tgaagctcac caagtccacg tcggtttcgc cgggcaagtc
    13321 ggaagtgcgc ttcaccgccg cggaatttcc cgcactgacg gtgaagaacc cgcgcctgtg
    13381 gtggccgaac ggctacggcg agcctgcgct gtaccacctc gcgcttgccg tcgcggatcg
    13441 cgggcgccct tcggacacga agtcgctgcg cttcggcatc cgccacgtca cctatgaatt
    13501 ctcgctgttc gattccgctg gtcgcctgcg ccgcgtcgaa gtggatgtgg ccggcggcag
    13561 cgcgcgcggt gagcgcctga tcgacattcg tcacgaagcg atcaagaaaa ctccgcaagg
    13621 ctgggcggca tcgctgacca aagtcggaga ggcttcgccc gccgtacgcg agctcgatga
    13681 tccactgcag tttccgcatc tcacgctacg cgtgaatggc gtgcgcatcc tcgcgcgcgg
    13741 cggcaactgg ggcatggacg attcgcgcaa gcgcatccag cgcgaacgtc tcgaacccta
    13801 cttccggctg cagaaagacg cgaacctcaa catcatccgc aactggatgg gcaccaacac
    13861 gcaggattcc ttctacgatc tctgcgatga atacggaatg atggtgctca acgacttctg
    13921 ggcgtcgacg caggatttcc aggtcgagcc gcaggatccg cagctcttcc tgcagaacgc
    13981 cgaggacacc gtgcagcgtt atcgcaacca cccgtcgatc gtgctgtggt tcggccgcaa
    14041 tgaaggtgtg ccgcagccga tcctcaatga aggactcggc gacgtcgtcg cgaagtacga
    14101 cggcacgcgg cacttcaccg gctcgtccaa caacgtgaac ctgcagggca gcggcccgta
    14161 taactaccgc ccgcccgtgg gttatttcac cgatctcgcg tgcggcttct cggtggagac
    14221 cggcacgccg tcgctggcga cactcgaagc catcgaggca atggttccca aggcggatcg
    14281 ctggccgctg tccgacacca tcgcctatca cgactggcat ttcggcggaa acggcgacat
    14341 caagacgttc atggcgacgt tggcgacgcg ttatggcgcg ggcacgagcc tggaagactt
    14401 cgagcgcaag gcgcagatga tgaactacga cacctatcgc gcgatgttcg aaggcctgca
    14461 ggcgcacctg tggaccaaga acagcggtcg gctgttgtgg atgacgcatc cggcgtggcc
    14521 gtcgaaccac tggcagatct actcgcacga ctacgacaca cacgcggcct acttcggtgt
    14581 gaagaaggcg gtcgaaccgg tgcacgtgca gatgaatctg ccggatttca gcgtcacggt
    14641 ggtcaacagc acgcgggaag cgcgtgacgg gctcaagctc gaattgcgcg tgtttaaact
    14701 cgatggtgtg cagcttttcg accacctgca cccggtcgac gccagggcca acgacatcac
    14761 accgctcggc gacctgggta ttgctgaacg tgtcgcgaag gaaggcatgg tgctggtggc
    14821 gctgcgtctg cgtgaccgca ctggcgcgct ggtgtccgac aacacctatt
//
LOCUS       DATDEU010000137         2816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2066667, whole genome shotgun sequence.
ACCESSION   DATDEU010000137 DATDEU010000000
VERSION     DATDEU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066667"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VIV63_03435"
     CDS             complement(<1..1161)
                     /locus_tag="VIV63_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123678.1"
                     /translation="MSTRSSTLIAAAVSLALAKHAAPAYAQEAATTSGEEIETVVVSG
                     IRASLGKSLEVKRESQAVVDVLTSEDVGKFPDKNVAEALQRVSGVSISREFGEGERVS
                     IRGTAPNLNRTLLNGHAIATADWFVLDQLSATRSFNYLMLPTEVIGSTEVYKGSQADI
                     EEGGIGGTVNIKTRNPLDLPSGLFTASLTGSYSDLADSTDPQIGALWSYKNDASTFGV
                     LLSGIYQERNIRRDGIEFLGYSDRVIADQGGATAAVPDLLGSALFEQDRVRTGFNFGL
                     QFRPSDAIDINVTGLYSKLDADNFNHNYMAWFSNMFGAGEQPTNTTVNAGGTLVGGTF
                     ANSASGWGVVYDAILRDAETETQGLDFDATFRSSDTLTWHTKVGYTEAEGATS"
     gene            1386..2129
                     /locus_tag="VIV63_03440"
     CDS             1386..2129
                     /locus_tag="VIV63_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY6123679.1"
                     /translation="MPTLSQVLPRLDESSSQPLYQQLQRALREAIENGVIGPDDALPP
                     ERDLADMLSVSRITVRKAIEELVEEGLLTRKQGSGTFVSNRVEKNFAKLTSFSEDMRA
                     RGREPRSVWLSRAEGTVTPEESLTLRSSPGTAVYRFSRIRYADDAPMALEYATVLRDC
                     LPSLETVETSLYEALEQNGNRPVRALQRLRAVLLTAEQAKLLKAQERDAGLLVARVGY
                     LKDGRAVEYSQSYYRGDIYDFVAELSASS"
     gene            2126..>2816
                     /locus_tag="VIV63_03445"
     CDS             2126..>2816
                     /locus_tag="VIV63_03445"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY6123680.1"
                     /translation="MTASTRMFREAAEGPQVVGAQLMLNAPLLRGLGARLRNDPPRAV
                     VTCARGSSDHAATYAKYLIESHTHVLTSSAAPSVSSIYDSQADLDGVLFIAISQSGKS
                     PDLLAATRHARDSGALTVALCNSPESPLMQVVDVAVPLHAGPETSVAATKSYIASLSC
                     IAQLVTNWTGDRKLLAALPQLPELLARAWKCDWTPAVEALRDAVNLFVVARGYGLGVA
                     QEAALKLKETCG"
BASE COUNT          505 a          864 c          903 g          544 t
ORIGIN      
        1 gctggtcgcg ccctcggctt cggtgtagcc gaccttggtg tgccaggtca gcgtgtcgct
       61 gctgcggaac gtggcatcga agtcgagacc ctgggtttcg gtttcggcgt cgcgcaggat
      121 ggcgtcgtac accacgcccc agccgctcgc gctattggcg aacgtgccgc cgaccagcgt
      181 gccgcccgcg ttcacggtgg tgttggtcgg ctgctcgccg gcaccgaaca tgttgctgaa
      241 ccacgccatg tagttgtggt tgaagttgtc ggcgtcgagc ttggagtaca ggcccgtgac
      301 gttgatgtcg atcgcatcgc tcggccggaa ctgcaggccg aagttgaagc cggtgcgcac
      361 gcggtcctgt tcgaacagcg ctgagcccag cagatccggt acggccgccg tggcgccgcc
      421 ctggtcggcg atcacgcggt cggaatagcc gaggaattcg atgccgtcgc ggcgaatgtt
      481 gcgttcctgg tagatgcccg agagcaacac gccgaacgtg ctcgcgtcgt tcttgtagct
      541 ccacaacgcg ccgatttgcg ggtcggtgct gtcggcgagg tcgctgtagg aaccggtgag
      601 cgacgcggtg aacaggccgg acggcagatc cagcggattg cgcgtcttga tgttcacggt
      661 gccgccgata ccgccttctt cgatgtcggc ctgcgagcct ttgtagacct cggtcgaacc
      721 gatgacctcg gtgggcaaca tcaggtaatt gaagctgcgg gtcgccgaca actggtcgag
      781 tacgaaccag tcggcggtcg cgatcgcatg gccgtttaac agtgtgcgat tcaggttggg
      841 cgccgttccg cggatggaaa cgcgctcgcc ctcgccgaac tcgcgtgaga tgctcacgcc
      901 gctgacacgt tgcagggctt cggccacgtt cttgtcaggg aacttgccga cgtcctcaga
      961 agtaagaaca tcaacgacgg cctgggattc acgtttgact tcgagcgact tgccgagact
     1021 ggcgcggata ccgctgacca cgacggtttc aatctcctcg cccgaggtgg ttgcggcttc
     1081 ctgtgcatac gcgggcgcgg cgtgtttcgc gagggccagc gagacggcag cggcaatcaa
     1141 cgtcgaactt ctggtgctca taaatcaggt tcccctattt ggttttcgta gataccaata
     1201 tggagccaat atagtaccaa tcagttcgaa aaaaccagtc tcttgcatac cattgtttcg
     1261 cagaatgggt ttgtcgcata aaaacagtgc gttatataag gcacgtgcat cacagaggtt
     1321 catattggta ttgctttggt tctatgcgaa tggcatggtt ccggtgagtt cagggggaaa
     1381 acgtcttgcc aacactttcg caggtactgc cgcggctcga tgaatcgagc tcgcagccgc
     1441 tttaccaaca actgcaacgc gcgctgcgcg aggccatcga aaacggggtg atcggtcccg
     1501 acgatgccct gccgcccgag cgcgacctcg cggacatgct ttcggtgtcg cgtatcaccg
     1561 tgcgaaaagc catcgaagag ctggtcgaag aaggactgct cacgcgcaaa caaggctcgg
     1621 gcacgttcgt gagcaaccgg gtggagaaga actttgcgaa gctcacttcc ttctccgagg
     1681 acatgcgcgc tcgtggccgt gagccgcgca gcgtgtggtt gagccgggcc gaaggcacgg
     1741 tgacgccgga agagtcactg accctgcgct cgagccccgg caccgcggtc tatcgcttca
     1801 gccgaattcg ttacgccgac gacgcgccca tggcgctcga atacgccacc gtgctgcgcg
     1861 actgcctgcc gtcgctggaa acggtggaaa cctcgctgta cgaggcgctg gaacagaatg
     1921 gcaatcgccc ggtgcgcgca ctgcaaaggc tgcgcgccgt tctgctgacc gcggaacagg
     1981 ccaaactcct caaggcccag gagcgcgacg ccgggttgtt agtcgccagg gtcggttatc
     2041 tcaaggacgg ccgcgcggtg gagtattcac agtcttacta ccgcggcgac atctacgatt
     2101 tcgtggcgga gctcagcgca tcatcgtgac cgcctcgaca cggatgttcc gcgaagcggc
     2161 cgaaggtccg caggtggtgg gcgcgcagct catgctcaat gcgccgctgc tgcgtgggct
     2221 cggcgcgcgt ctgcgcaacg accccccgcg cgccgtcgtg acctgcgcgc gcggcagttc
     2281 cgatcacgca gcgacctacg cgaaatacct gatcgaatcg cacacgcacg tgctcacttc
     2341 gtcggcggcg ccatcggtga gctcgatcta cgactcacag gcggatctcg acggcgtgtt
     2401 gttcatcgcc atttcgcagt ccggcaagag ccccgacctg ctcgccgcca cacgccatgc
     2461 gcgcgacagc ggcgcgctga ccgtggcctt gtgcaactcc ccggaatctc cgctcatgca
     2521 ggtcgtcgac gtcgccgttc cgctgcacgc cggacctgag acaagcgtgg ctgccacgaa
     2581 gtcgtacatc gcttctctga gctgcatcgc gcagctggtc acgaactgga ctggggaccg
     2641 aaagttgttg gccgctctgc cgcagctgcc ggagctactg gcgcgcgcct ggaaatgcga
     2701 ttggactccc gcggtggagg cgctgcgcga cgccgtgaat cttttcgtgg tcgcacgggg
     2761 ctatggcctc ggcgtcgcgc aggaagcggc gctcaaactc aaggaaacct gcgggc
//
LOCUS       DATDEU010000138        11845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1774621, whole genome shotgun sequence.
ACCESSION   DATDEU010000138 DATDEU010000000
VERSION     DATDEU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774621"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VIV63_03450"
     CDS             complement(<1..230)
                     /locus_tag="VIV63_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6123681.1"
                     /translation="MIREISVGELKARRDRGEKPLVIDVREDWELKLASIPGVVHVPM
                     NQLPARLGEFQRDAETIVMCHAGGRSMRVAHFL"
     gene            complement(237..887)
                     /locus_tag="VIV63_03455"
     CDS             complement(237..887)
                     /locus_tag="VIV63_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY6123682.1"
                     /translation="MQIEFARTQMITQQIRAWDVLDERVLDAMRRTPREFFVPERYVD
                     VAFADTDIPLRAGQHMLAPKIVGRLLQALAAEPGMRALVVGSGSGFVPACLSAMGASV
                     RAIEIDGELAIASRANLKRAGFGQVDVVTGDTFNLDMGKDYALIAVCGALPLYDDRFA
                     RALAVGGKLFVVVGSELPQEALLVTRTAESAWSSTALFETAVDPLDNARHPEAFQF"
     gene            1093..3471
                     /locus_tag="VIV63_03460"
     CDS             1093..3471
                     /locus_tag="VIV63_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6123683.1"
                     /translation="MSILGLICVLLALVATLLFVLFLFQRRDLRAISELSLTVQRAIS
                     GERLPQRIEISDEHESQLDVQALGVSINQLLLRASRTTGREKAGPKLFTELGERIHEA
                     VLVHRDVILYANSQFASFVGVDRVDLIDRRLSDLVAPEYAELVGENLRRRIAGSHGAE
                     RFEVEMVGLQGQVSLLELTTAQIDFEGAPALLVTGVEVIPTRKLRALASGKIDEKEAA
                     ARAALVAPPLPPPPPTPPPLQVFAMQSLSEAIVTTDIEGRLAYLNPAAERLLGVVAAQ
                     VIGRPLEDVVGLVDQNDRKQLSDPVKEAVGGGTGNPHNLSRRAVLLGKSSGEERAIEL
                     AASPLRVEDELVGAVILLHDVTELRGLHRQMSYQATHDALTGLVNRREFERRLDEGTE
                     AARRGEAAHMLCYLDLDRFKIVNDTSGHLAGDSMLREVAKLLREAVRDSDTVARLGGD
                     EFGMLLVGCPLDKARQIADDVCRSIATYRFVWHDRVFNIGVSIGLIEIGREAGSVEQL
                     LAAADSACYVAKKEGAGRVSVYSARDEALARSTGEIEWLQKLQSAIKEERFALYYQPI
                     VSAYGNETDGPSMEVLLRMLDETGAEIAPLEFVGAAERYRLMTSVDRWVVQTTLVALS
                     RNAFQLARDRSVAINISAQTLGDPLFLEFVVECLDRTGVSPGQVCFEIAESAVISNME
                     HARRFVGVLHGMGCKFTIDDFGSGVASFSSLKNLPLDYLKLDGSFMRNLARDSVSQTM
                     VTAMIKLARTLNFKIIAEQVEDSAALDVARKMGVDFVQGFVIARPAKLALAA"
     gene            3516..3749
                     /locus_tag="VIV63_03465"
     CDS             3516..3749
                     /locus_tag="VIV63_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123684.1"
                     /translation="MSVAQSQGSAPCRFRGQHPTVARPASRISLVSPAARERAGIWCA
                     ATQRGLRASSRPRLRDSRQRRPAAGNSPRRRTS"
     gene            3801..4328
                     /locus_tag="VIV63_03470"
     CDS             3801..4328
                     /locus_tag="VIV63_03470"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY6123685.1"
                     /translation="MRLPTARPGCVLRPWAPADKASLIANANNRNVWRNLADVFPHPY
                     TDADADAWFNLVSTPDRSITLAIEVDGMAVGGIAARSGTGIFVRTGHFGYWLGEPYWG
                     QGIVTAAGRALLDFLKQDARFVRLEAPVFEWNPASMRVLEKLGFERVALLRKSVTKDG
                     QLIDSVMYSCLLNAS"
     gene            4341..4745
                     /locus_tag="VIV63_03475"
     CDS             4341..4745
                     /locus_tag="VIV63_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6123686.1"
                     /translation="MLKTYQGSCHCGAVKFEADIDLALGTNKCNCSICTKTRNWNAII
                     KPDAFRLISGEDAVTDYRFGSKQGSHLFCRTCGVRSFERGHVKEIGGDYVSIKIASLD
                     DVELDEIIGAPVRYANGRDNAWWEPPKETRHL"
     gene            4759..5421
                     /locus_tag="VIV63_03480"
     CDS             4759..5421
                     /locus_tag="VIV63_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123687.1"
                     /translation="MKIVREISRRRVLLGTGACATVAALGGTAAAADKLRRTPTQVLG
                     PFYPLDKPLDADADLTLVKGRKGRAEGQVLHVMGRVLNADGVPVSGARIEIWQANARG
                     RYAHPADNSPAPLDPNFQGYCQLLTDADGRYRFKTIKPLGYPAGDIMRPAHIHFDVMG
                     KHNRLVTQMYFPDDPMNDSDVVLATATKNRKLLVADVRAPTPDLEPDSQLAHWDIVLE
                     NG"
     gene            complement(5428..6249)
                     /locus_tag="VIV63_03485"
     CDS             complement(5428..6249)
                     /locus_tag="VIV63_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6123688.1"
                     /translation="MEVATSSILILLVIATVCAVAGIALLGRVPRHNAGFQILRLIAW
                     LLLAFGTGLTALIAGLQLTGRLGPAQLWFRAPTFYGAAAILVLIAAWWFRRTLVHPAA
                     RLIRFGLPALALVLLGMLAIVQRLDGRSTPLSALMPTLRAEAPELSFHDSGGAIRRLS
                     EYRGKVVLVNFWATWCGPCRHEMPMLSSAQADFGGQGLVVIYLSLEEPEILEPFLRTH
                     HFEGIQGRLAQAADYYRAGQIYPLSYLISRDGRVAKRWSGRPAEGWLRDSIREEL"
     gene            complement(6326..7054)
                     /locus_tag="VIV63_03490"
     CDS             complement(6326..7054)
                     /locus_tag="VIV63_03490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6123689.1"
                     /translation="MSSVSDHYSQLLAPVYTWMSGSVEAALDAGKSEIDELRLQLPAG
                     ALVADLGAGFGKHAIPLARAGMRVLAVDSSTELLTELDRLTGGLSVETVADDLLSFRT
                     HLKEKAAAVFCMGDTLTHLPEHTHVDFLVQEVHEALAPGGYFVLSFRDYSEPLVGDAR
                     FIPVRSDERRILTCFLEYEEDTVVVHDILHERAGDAWETRVSSYRKLRLSVDRVVASL
                     ETIGFETRKQSGLRGMVQIIGTIP"
     gene            complement(7051..7650)
                     /locus_tag="VIV63_03495"
     CDS             complement(7051..7650)
                     /locus_tag="VIV63_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock and DUF1294 domain-containing
                     protein"
                     /protein_id="HEY6123690.1"
                     /translation="MRDQGRLTDWNDEKGFGFVTPDNGGLRAFVHIRAFSYRTRRPRN
                     GDVITYQLTWDPKGRPRAENVLFRGMTKPPSQPEPTHVVTASLLVAVFIAAVTALAIA
                     GRIPWFLPLVYLAVSLLTFCVYAFDKSAAMNRRWRTSEETLQGLSLFGGWPGALIAQR
                     LFFHKSKKADFQAGFWIIVVIHCAAVFLFGMRYVELPFR"
     gene            complement(7663..9189)
                     /locus_tag="VIV63_03500"
     CDS             complement(7663..9189)
                     /locus_tag="VIV63_03500"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY6123691.1"
                     /translation="MPMPETGEALELLAASLTRARGDNFFPALAAHLGEVLGATQSMV
                     CEAAANRRARTLGVWRDGAVASNYDYDLEGTPCAKVQAGQALAVELTPGSFPGAPQQR
                     GGYFGMPLAANDGAILGHFCAYLERPLPLSQTQRAVCDILANRAAAELRLVHVKRERA
                     LLRGQRRQLRAEIAALHNIDGLQGASAAHLRMMEEVRRAALISANVLVRGEAGTGKEL
                     VARTIHAGSARVNKPFVKIDCAALSIDADINALPQMLALAIGGTLFLDEVGVLSADMQ
                     TRLLAALPTSHGEPGSTNNINVRVLASSSRDLFRKVRDGDFLEGLYGRIAMLAIDIPP
                     LRTRVEDIPLLVQEFVHKRARRMGRRVQSVDPQSLAELANYGWPGNIRELENLVERAL
                     VMQDAPVLKITTDFLGTTAPAERAALVAAAGDSTSITRTTLAGPVDFDDTLSTGLHVV
                     QRDHILRVLNATHWVIEGSHGAALKLGLKPATLRHRMKKLGISRAANAGAPTSGGDAR
                     "
     gene            complement(9312..9767)
                     /locus_tag="VIV63_03505"
     CDS             complement(9312..9767)
                     /locus_tag="VIV63_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6123692.1"
                     /translation="MNSLTQAIHGHTAFANLLDRLRPWLLLGTRLWVSWQFLKSGWLK
                     LTTWDTTLELFSSEYQVPVLSPGVAAVLGTAGELFFPTLLVLGLFTRISALGLLAVNA
                     MAVISYWHVLGSEGFEAALAQHVLWGYMLAVILLCGAGALSLDEKLARR"
     gene            10065..10433
                     /locus_tag="VIV63_03510"
     CDS             10065..10433
                     /locus_tag="VIV63_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123693.1"
                     /translation="MQLSQMWREHLQEGCPAEMRSMNIAGMSAGALDAEITACVSACV
                     THSFRMDSRIERRLHDIAHALEDFQGQGPPEVAAYCTRLNKLVSAVLVNAGPTETVKE
                     TGESDPTAARQPVRPRMRRR"
     gene            complement(10485..10886)
                     /gene="msrB"
                     /locus_tag="VIV63_03515"
     CDS             complement(10485..10886)
                     /gene="msrB"
                     /locus_tag="VIV63_03515"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY6123694.1"
                     /translation="MSDETFPVEKSEQEWRKELSADQFRVLREHGTERAGASPLNFEK
                     RTGVFHCAACNQPLFESSTKYESGSGWPSFWKPLPGAVATTTDTTHGMTRVEVHCKNC
                     GGHLGHVFDDGPQPTGQRYCMNGLSLKFEPK"
     gene            complement(11019..>11845)
                     /locus_tag="VIV63_03520"
     CDS             complement(11019..>11845)
                     /locus_tag="VIV63_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY6123695.1"
                     /translation="TGMVVALDAYGPITDNAGGIAEMAGLDKKIRDITDPLDAVGNTT
                     KAVTKGYAIGSAGLAALVLFADYTHNLETHLVATGREMVQFSLSDPAVIIGLFIGGLV
                     PYLFGAMAMEAVGRAAGSVVEEVRRQFREIKGIMEGTAKPEYGRAVDLLTKAAIKEMI
                     IPSLLPILIPVVIAFGMTWLMGPGAGIKALGGLLIGTIVTGLFVAISMCTGGGAWDNA
                     KKYIEEGNFGGKGSDAHKASVTGDTVGDPYKDTAGPAINPLIKIINIVALLLVPLL"
BASE COUNT         2186 a         3720 c         3857 g         2082 t
ORIGIN      
        1 agaaaatggg cgacccgcat tgatctgccg cccgcgtggc acatgactat ggtttccgca
       61 tcgcgctgaa actcccctaa acgggccggt aactggttca tcggcacatg tacgacgccc
      121 ggaatgctcg caagtttcag ctcccagtcc tcgcgtacgt cgatcaccag cggcttttcc
      181 ccgcggtcgc ggcgcgcctt gagttccccg accgagatct cgcggatcat ggcgctctag
      241 aactggaagg cttcggggtg gcgggcattg tccagcgggt ccacggcggt ttcgaacaac
      301 gcggtgctgc tccaggcgct ctcggccgtg cgcgtgacca gcagggcttc ctgcggcaac
      361 tcgctgccga ccacgacgaa cagcttcccg cccacggcta acgcgcgagc aaagcgatcg
      421 tcgtacaggg gtagggcgcc acataccgca atgagcgcat agtcctttcc catatccaag
      481 ttgaaagtgt cacccgtgac cacgtcgacc tggccaaacc ccgcacgttt cagattggcc
      541 cggctcgcga tagcaagctc gccgtcgatc tcgatggcgc ggacgctggc gcccatcgcc
      601 gagagacacg cgggcacgaa cccggatccc gacccgacca cgagcgcgcg cattccgggc
      661 tccgcggcca gcgcctgcag gaggcgtccc acaattttcg gcgcgagcat gtgctggccc
      721 gcgcgcagcg gaatatcggt gtcggcgaag gcgacatcca cgtagcgttc gggcacgaaa
      781 aactcgcgcg gcgtgcgacg catggcgtcg agcacgcgtt cgtcgagcac gtcccacgcg
      841 cgtatctgct gcgtgatcat ctgcgtgcgc gcgaactcga tctgcatgtg caactcctac
      901 aaactggatg ctacttattc tgcccgaatc gacgacttgc agtggttcgt ttcgtcgcgg
      961 aaccagtcgt cacatcgctt atttttatgg ttaatgtcga atgagagctc gaccggataa
     1021 ggctattctg gccgcgggca cggatgctcc gcacaaaaag atgcactaat aacaacagga
     1081 ttacgtttcg ggatgtcgat tcttgggttg atctgcgtgc tgctcgcgct ggttgcgacg
     1141 ctgctcttcg tgctgttctt atttcagcgg cgcgatttgc gggcgatctc cgagttgtcg
     1201 ctcacggttc agcgtgcgat cagcggcgag cgtctgccgc agcgcatcga aatttccgac
     1261 gaacacgaat cacagctcga cgtgcaggcg ctcggcgtgt cgatcaacca gttgttgctg
     1321 cgcgcctcgc gcacaacggg tcgcgagaaa gccggcccga aactcttcac cgaactcggc
     1381 gagcggattc acgaagcggt gctcgtgcat cgcgacgtca tcctgtatgc caacagccag
     1441 tttgcgagtt tcgtcggcgt ggatcgcgtc gacctcatcg atcgccgtct gtccgatctc
     1501 gtggcgcccg agtacgccga actggtcggc gagaacctgc gccgccgcat cgccgggtcg
     1561 catggcgccg aacgtttcga ggtggagatg gtcggcctgc agggccaggt gagcctgctc
     1621 gaactaacta ccgcgcagat cgacttcgaa ggagcgcccg cgctgctcgt gaccggcgtc
     1681 gaagtcatcc ccacgaggaa gctgcgcgcg ctcgccagcg gcaagatcga cgagaaggag
     1741 gctgccgcgc gtgccgcgct ggtcgcgccg ccgctcccgc cgccgccgcc cacgccgccg
     1801 ccgctgcagg tgttcgccat gcagtcgctg agcgaagcca tcgtcaccac ggacatcgag
     1861 ggacgcctcg cgtatctcaa ccccgctgcc gagaggctgt tgggcgtggt cgccgctcag
     1921 gtgatcggcc gtccgctcga agacgtcgtc ggcctcgtcg accagaacga tcgcaagcag
     1981 ctcagcgatc cggtcaagga agccgtcggt ggcggcacgg gcaatccgca caatctcagc
     2041 cgccgcgccg tgttgctcgg caaatcttcc ggggaggagc gcgccatcga gctcgccgca
     2101 tcgccgctgc gcgtcgagga cgaactcgtt ggcgcggtga tcctgctgca cgacgtcacc
     2161 gaactgcgtg gcctgcaccg gcagatgtct tatcaggcga cccacgacgc gctgaccggc
     2221 ctcgtgaacc ggcgcgagtt cgagcgccgt ctcgacgagg ggaccgaagc ggcgcgccgc
     2281 ggcgaggccg cgcacatgtt gtgttacctc gatctcgatc gcttcaagat cgtcaacgac
     2341 accagcggcc atctcgcggg cgacagcatg ttgcgcgaag tggccaaact gctgcgcgaa
     2401 gcggtgcgcg actcggacac ggttgcgcgt ctcggtggtg atgaattcgg gatgcttctc
     2461 gtgggttgcc cgctcgacaa ggcgcggcag atcgccgacg acgtatgccg ctccatcgcg
     2521 acgtatcgct tcgtgtggca cgaccgcgtg ttcaacatcg gcgtgtccat cggcctcatc
     2581 gaaatcggcc gcgaagccgg ctccgtcgag caactgctgg ccgccgcgga ctcggcctgc
     2641 tacgtcgcca agaaagaagg cgcgggccgc gtgtccgtct actcggcgcg cgacgaggca
     2701 ctggcacgca gcaccggcga aatcgaatgg ctgcagaagc tgcagtcggc catcaaggaa
     2761 gagcgcttcg cgctgtacta ccagcccatc gtctcggcct atggcaacga gaccgacggt
     2821 ccgtcgatgg aagtcctgct gcgcatgctc gatgaaaccg gcgccgaaat cgcgccgctc
     2881 gaattcgtgg gcgccgccga gcggtaccgc ctcatgacgt cggtggatcg atgggtggtg
     2941 cagacgacgc tggtcgcgtt gtcgcgcaac gccttccagc tcgcgcgtga ccgcagcgtg
     3001 gccatcaaca tctccgctca gaccctggga gacccgctgt tcctcgagtt cgtggtcgaa
     3061 tgcctcgacc gaacgggcgt ctctcccggc caggtgtgct tcgagatcgc cgagagtgcg
     3121 gtcatcagca acatggagca cgcgcggcgt ttcgtcggcg tactgcacgg catgggctgc
     3181 aaattcacga tcgacgattt cggttcgggc gtcgcgtcgt tttcaagcct caagaacctg
     3241 ccgctcgatt acctcaaact cgacggctcg ttcatgcgca acctggcgcg cgattcggtg
     3301 agccagacca tggtgaccgc gatgatcaag ctcgcgcgta cgctgaattt caagatcatc
     3361 gccgagcagg tggaagacag cgccgcgctc gacgtcgccc gaaagatggg cgtggatttc
     3421 gtgcaagggt tcgtgatcgc ccggcctgcg aaactcgcgc tcgcggcctg atcacctttt
     3481 aactaacaag attgccgatt cggggagttt tcaagatgtc agttgcacaa agccagggca
     3541 gcgcgccgtg ccgcttccgc gggcaacatc ccaccgtggc gcgccctgcg tcgcgtattt
     3601 cactcgtcag ccccgccgca cgcgagcgtg ccggcatctg gtgcgcggcc acgcagcgcg
     3661 gcctgcgggc cagcagccgt ccgcgcctgc gtgattcgcg ccagcgccgt ccagccgcgg
     3721 gcaattcgcc ccgtcgccga accagctagt cgaacgcgcc gctctcggcg atcattcgcc
     3781 cctgccattg cgaggggcga atgagactgc caaccgccag accgggatgt gtattgcggc
     3841 cgtgggcgcc ggccgacaag gccagcctca tcgccaacgc caacaatcgg aacgtctggc
     3901 gaaacctcgc cgacgtattt ccgcatccct acaccgacgc cgacgccgac gcgtggttca
     3961 acctcgtgag cacaccggat cgcagcatca cgctcgccat tgaagtcgat ggcatggctg
     4021 tgggtggtat tgccgcgcgc tcgggaactg ggatcttcgt gcgcaccggg catttcggtt
     4081 actggctcgg agaaccctac tggggacagg gcatcgtcac ggcagcgggg cgtgcgttgc
     4141 tcgactttct gaagcaggac gcgcgcttcg tgcgactcga agcgcctgtg ttcgagtgga
     4201 acccggcatc catgcgggtg ctcgaaaaat tgggcttcga gcgcgtggcg ttgctgcgaa
     4261 agagtgtcac caaggacggg cagctcatcg acagtgtgat gtactcctgt ctgctgaatg
     4321 cttcctgacg gagagccaac atgttgaaga cctaccaggg cagctgtcat tgcggcgccg
     4381 tgaagttcga agcggacatc gatctcgcgc tgggcaccaa caagtgcaat tgcagcatct
     4441 gcaccaagac gcggaactgg aacgccatca tcaagcccga tgcgttccgc ctgatatcgg
     4501 gtgaagacgc ggtgactgac taccggttcg gcagcaagca gggctcgcat cttttctgca
     4561 ggacttgtgg cgtgcggagt ttcgagcgtg gccacgtcaa ggagatcggc ggcgactacg
     4621 tgtcgatcaa gatcgcgagc ctggacgatg tcgagctcga cgaaatcatc ggggcgcccg
     4681 tgcgctacgc gaacgggcgc gacaacgcct ggtgggaacc gccgaaagag acgcgccacc
     4741 tttgacaatg gagatcacat gaagatcgtg cgagaaattt cccggcggcg cgtactgctg
     4801 ggcacggggg cttgcgcgac ggtcgcggca ctcggcggca cggccgccgc ggccgacaag
     4861 ctccggcgca cgcccaccca ggtgttggga ccgttctatc cactcgacaa gccgctcgat
     4921 gccgacgccg atctcactct cgtcaagggc aggaaggggc gggccgaggg ccaggtgttg
     4981 cacgtgatgg gccgcgtgtt gaacgcggat ggcgtacctg tgagtggcgc gcgaatcgaa
     5041 atctggcagg cgaatgcgcg cggacgttac gcccatcccg ctgacaacag cccggcaccg
     5101 ctcgatccga attttcaggg ctactgccag ttgctcacgg atgccgacgg ccgataccga
     5161 ttcaagacca tcaagccgct gggttatccg gcgggcgaca tcatgcgtcc ggcgcacatc
     5221 catttcgacg tgatgggaaa gcacaatcgc ctggtgacgc agatgtattt tccggacgac
     5281 ccgatgaatg acagcgatgt ggtgctggcc acggccacca agaaccggaa actcctcgtg
     5341 gctgacgtcc gcgcgccgac gccggacctg gagccggatt cacagctggc gcactgggac
     5401 atcgttctcg agaacggctg accgaatcta aagttcctcg cgaatcgagt cgcgaagcca
     5461 tccttcggcg ggccggccgc tccagcgctt ggcgacgcgg ccgtcgcgac tgatgagata
     5521 actcaacggg tagatctggc ccgcgcggta atagtcggcc gcctgcgcga ggcggccctg
     5581 tatgccttcg aaatggtgcg tgcgcaggaa cggctcgagt atttcgggct cctcgagcga
     5641 tagatagatc accaccaggc cttgcccgcc gaaatccgcc tgcgcgctcg acagcatcgg
     5701 catctcgtgc cggcaggggc cgcaccacgt cgcccagaaa ttcacgagca cgaccttgcc
     5761 gcgatattcg gagagccggc gaatggcgcc gcccgaatcg tggaaactca attcgggcgc
     5821 ttccgcgcgc agcgtcggca tcagggccga caacggagtc gagcggccat cgaggcgttg
     5881 cacgatcgcc agcatcccga gcaacaccag cgccagcgcg ggcagtccga agcgaatgag
     5941 tcgcgcggcg gggtgtacca acgtgcggcg gaaccaccag gcggcgatca acacgaggat
     6001 tgctgccgca ccataaaaag taggcgcgcg aaaccagagt tgcgcggggc ctagccgacc
     6061 ggtgagttgc agcccggcga tcagtgcagt caaccccgtg ccgaacgcga gcaggagcca
     6121 ggcgatcaat cgaaggatct ggaagccggc gttgtgccgt gggacccggc ctaacaacgc
     6181 aatgccggcc acggcgcata cggtcgcaat gaccagcagg atcaggatgg atgaggtggc
     6241 tacttccatg cggcgtggat tgtcgcatcg ccttccggtg ggccgggctc aacccgctgt
     6301 aagaattgtc ctcaaggaat ggccctcaag ggatggtccc gatgatctgg accatgccgc
     6361 gcaggccgga ttgtttgcga gtttcgaatc cgatggtctc gaggctggcg acgacgcgat
     6421 ccacggacag ccggagcttg cgatagctgc tgacgcgcgt ttcccaggcg tcgccggcgc
     6481 gctcgtggag gatgtcgtgc accaccaccg tgtcctcttc gtattcgagg aagcaggtga
     6541 gtatgcggcg ctcgtcgctg cgcaccggga tgaaacgcgc atcaccgacg agcggctcgc
     6601 tgtagtcgcg gaacgagagc acgaagtagc cgccgggggc gagcgcctcg tggacttcct
     6661 gcacgaggaa atcgacgtgc gtgtgttcgg gaagatgcgt gagcgtatcg cccatgcaga
     6721 aaaccgcggc agccttctcc ttcagatgcg tgcgaaacga cagcagatcg tcggccacgg
     6781 tttcgacgga gaggccgccg gtgagccggt cgagctcggt gagcagctcc gtcgaactat
     6841 ccactgccag cacgcgcatg cccgcgcgtg cgagcgggat ggcgtgtttg ccaaaccccg
     6901 cgccgaggtc ggcgaccagg gcgccggccg gcaattgcaa tcggagctcg tcgatttcgg
     6961 atttgccggc gtcgagcgcg gcttcgacgc tgcccgacat ccaggtgtag accggggcga
     7021 gcagctgtga gtagtggtcg gagacggagc tcatctgaaa ggcaactcca cgtagcgcat
     7081 gccaaacaag aataccgcgg cacagtggat cacgacgatg atccagaaac ctgcctggaa
     7141 gtccgccttc ttcgacttgt ggaaaaacag ccgttgcgcg atcagggctc ctggccatcc
     7201 gccgaacaga ctcaagcctt gcagcgtctc ttcgctcgtt ctccagcgac gattcatggc
     7261 cgccgacttg tcgaatgcat agacacagaa agtcaggaga ctcaccgcga ggtacaccaa
     7321 gggcaagaac cagggaatcc ggccagcgat ggcaagcgcg gtgaccgccg cgatgaacac
     7381 cgccaccagc aaggaggcgg tcaccacatg cgtcggttcc ggttgcgaag gaggcttggt
     7441 catcccgcga aacaacacgt tctccgcgcg cggtcggccc ttcgggtccc aggtgagttg
     7501 ataggtgatg acgtcgccgt tgcgcggccg gcgtgttcga tatgaaaacg cacggatatg
     7561 gacgaaggcg cgaagccccc cgttatcagg ggtgacgaat ccaaaccctt tttcgtcgtt
     7621 ccagtcggtc aatcgcccct ggtcgcgcat cgcggctcgg tgttacctgg cgtcgccgcc
     7681 ggaggtcggc gcccccgcgt tcgccgcgcg cgagatcccc agcttcttca tgcggtggcg
     7741 caatgtcgcc ggcttgagcc cgagcttgag cgccgcaccg tgactgcctt cgatgaccca
     7801 gtgggtggcg ttgagcaccc gcaggatgtg atcacgctgt acgacgtgca gtccggtgct
     7861 cagtgtgtcg tcgaaatcca ccgggcccgc gagagtggtc cgcgtgatgg atgtcgaatc
     7921 gcccgcagcg gcgacgagcg cggcgcgttc cgccggcgcc gtggtgccca gaaaatcggt
     7981 ggtgatcttg agcaccggcg cgtcctgcat gaccagcgcg cgttcgacga gattttcgag
     8041 ctcgcggata ttgccgggcc agccgtagtt ggcgagttcg gccagcgatt gcggatcgac
     8101 gctctggacc cgccgtccca tcctgcgtgc tcgcttgtgc acgaactcct gcaccagcag
     8161 cggaatatcc tcgacgcggg tacgcaaggg cgggatgtcg atggccagca tggcgatgcg
     8221 gccgtagagt ccttcgagga agtcgccatc gcgcactttg cggaagagat cgcggctgct
     8281 cgaagcgaga acacgcacgt tgatgttgtt ggtgctgccg ggttcgccgt gcgaagtggg
     8341 cagcgcggcc aacagcctgg tttgcatgtc ggcggaaaga acaccgacct cgtcgagaaa
     8401 caacgtaccg ccgatggcca gcgccagcat ttgcggcagg gcgttgatgt cggcgtcgat
     8461 cgacagtgcg gcgcagtcga tcttcacgaa aggcttgttg acgcgtgcac tgccggcgtg
     8521 gatggtgcgg gcgacgagtt ccttgccggt gccggcttcg ccccgtacca gcacgttggc
     8581 ggaaatcagc gcggctcgcc gtacttcttc catcatgcgc aggtgcgcgg cgctcgcgcc
     8641 ctgaagtccg tcgatgttgt gtaaggcggc gatttccgcg cgaagctgcc gtcgttgtcc
     8701 gcgcaggagg gcgcgctcac gcttgacgtg gacgagccgc agttcggcag cggcgcggtt
     8761 cgcgagaatg tcgcagacgg cgcgctgggt ctggctgagc ggcagcggcc gttcgaggta
     8821 ggcgcagaaa tggccgagaa ttgcgccgtc gttcgcggcg agcggcatac cgaagtagcc
     8881 gccacgttgc tgcggcgcgc ccgggaacga gcccggcgtc agttcgacgg ccagcgcctg
     8941 gcccgcctgc accttcgcgc acggcgtgcc ctcgagatcg taatcgtagt tagacgccac
     9001 cgcgccgtcg cgccacacgc cgagcgtgcg cgcccggcgg ttggccgcgg cttcgcagac
     9061 catggactgc gtggcaccca gcacttcccc gagatgagcg gccagcgcgg gaaaaaaatt
     9121 gtcgccgcgg gcgcgagtca ggctcgccgc cagcagttcg agagcttcgc ccgtttcagg
     9181 catcggcatt tcaccaggac tctaataaga gtacggcgag tataggcgag cctttgcgcg
     9241 caacgaaatg cggccgcgtc gcgcaccttc tcagggcgtc acgtaggcac accgcctgcc
     9301 ggcatccagg atcagcggcg agctaacttc tcgtcgagcg aaagtgctcc ggcaccgcac
     9361 aacaggatca ccgcgagcat gtaaccccag agcacatgct gcgcgagcgc tgcttcgaaa
     9421 ccttcggagc cgagcacgtg ccagtacgag atgacggcca tcgcattgac cgcgagaagt
     9481 ccgagggcgc tgatccgggt gaacaggccg agcacgagta gcgtcgggaa gaacaattcg
     9541 ccggccgtcc cgaggaccgc ggcgacgccc ggcgacagga cgggcacctg gtactcgctg
     9601 ctgaacagtt cgagcgtggt gtcccaggtc gtgagtttca gccagccgga cttgaggaac
     9661 tgccagctca cccacaggcg ggtgcccagc agcagccagg ggcgaaggcg gtcgaggaga
     9721 ttcgcgaagg cggtgtggcc atggatggct tgcgtcaggc tgttcatcga gactcccctg
     9781 ggttgttagc ggtctagacc ggggacagga agccgatatt gcgcaatgac cggcaaaact
     9841 ttgcgaatgg ccctggcgca acgcgcaaag atcgacgcga ggatgtccgg aatcaaccac
     9901 ttaaggcgtc ttctgttacc ggatgtttgc gcgtgtggga ttggtgccac ggacagttct
     9961 gcgcggcgca cctcctgcag cacgcggcac gggaatttcc gcgggacgct tgctgcaatt
    10021 tgcggtaatt tcgcccgcaa tccggacttg gggagacgag agcgatgcaa ctgtcgcaaa
    10081 tgtggcgtga gcatctgcag gaaggctgtc cggccgaaat gcgcagcatg aatatcgcgg
    10141 gcatgtcagc gggtgcgctg gatgccgaaa tcactgcgtg tgtgagcgcg tgtgtaacgc
    10201 actcgttccg catggactca cgaatcgagc ggcgcctgca tgacatcgcg cacgcgctcg
    10261 aagattttca ggggcaggga ccgccggagg ttgctgcgta ctgcacccgt ctcaacaagc
    10321 tcgtcagcgc cgtgctcgtg aatgcggggc cgaccgaaac ggtgaaggaa actggcgaga
    10381 gcgatccgac ggccgcccgt cagccggtgc ggccgcgaat gcgtcgccga taagaaatgg
    10441 ggtcagaccc catttcctag caaaaggggc cggacctcta cgttctactt cggctcgaac
    10501 ttcagcgaca agccattcat gcagtagcgc tggccggtcg gctgcgggcc gtcatcgaac
    10561 acgtggccga gatggccgcc gcagttcttg caatgcactt ccacgcgcgt catgccatga
    10621 gtggtgtcgg tcgtcgtggc cacggcgccg ggcaacggtt tccagaagct gggccagccc
    10681 gaaccgcttt catacttggt gctggattcg aacagcggct gattacacgc cgcgcagtgg
    10741 aacacgccgg tgcgcttctc gaagttgagc gggctggcgc cggcgcgctc cgtgccgtgt
    10801 tcgcgcagca cgcggaactg gtccgcggac aattccttgc gccattcctg ctctgatttc
    10861 tcgaccggga acgtctcgtc cgacatatga ggtgctcctt cgcgcatacc aacaggaccg
    10921 ggtatgtggg gacgatattt cctcatgcca gaaagcggaa ggggacgaag cctttcgagc
    10981 cgcgtcccct tttgctagca aatcaggaat gtccccattt acaggagtgg caccagcagc
    11041 agcgccacga tgttgatgat cttgatgagc gggttgatgg ccgggccagc ggtgtccttg
    11101 tagggatcgc cgacggtgtc gcccgtgacc gaagccttgt gcgcatcgct tcccttgccg
    11161 ccgaagttgc cttcctcgat gtacttcttg gcgttgtccc aggcgccgcc gcccgtgcac
    11221 atggagatgg cgacgaacag tccggtgacg atggtgccga tcagcaatcc gcccagcgcc
    11281 ttgatgccgg cgcccggacc catcagccag gtcatgccga aagcgatgac caccggaatc
    11341 aggatgggca gcagcgaagg aatgatcatt tccttgatcg cggccttggt gagcaggtcg
    11401 acagcgcggc cgtactcggg cttggccgtg ccttccatga ttcccttgat ctcgcggaac
    11461 tgacgacgca cctcttcgac cacggatccc gcggcgcggc ccacggcttc catggccatg
    11521 gcgccgaata gatagggcac gagtccgccg atgaacaggc cgatgatcac ggccggatca
    11581 gacagcgaga actgcaccat ctcgcgtccg gtggcgacca gatgagtttc caggttgtgc
    11641 gtgtagtcag cgaacagcac gagagccgcg aggcccgccg agccgatggc gtaccccttg
    11701 gtcacggcct tggtggtgtt gcctaccgcg tcgagcggat cggtgatgtc gcggatcttc
    11761 ttgtcgagtc cggccatctc ggcgatgccg ccggcgttgt cggtgatcgg gccgtaggcg
    11821 tcgagcgcca cgaccatgcc ggtga
//
LOCUS       DATDEU010000139         2604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1337438, whole genome shotgun sequence.
ACCESSION   DATDEU010000139 DATDEU010000000
VERSION     DATDEU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337438"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..88)
                     /locus_tag="VIV63_03525"
     tRNA            complement(3..88)
                     /locus_tag="VIV63_03525"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Tyr,seq:gta)
     gene            complement(237..773)
                     /locus_tag="VIV63_03530"
     CDS             complement(237..773)
                     /locus_tag="VIV63_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123696.1"
                     /translation="MKNHLPLSFLGALVVAGPVAAEVRETRENGFTIETTVMAEAKPA
                     AVYRKLIKVAGWWDPAHTWSGSAANLRLWPEAGGCFCEKLAHNGSVEHARVIFAQPGK
                     MLRLEGALGPLQDMAVSAVLTFTLAPDGPGTRIKMTYRVAGALTMDSAKLAPLVDQVM
                     GTQLGRLRSFASGAPAGH"
     gene            complement(787..1536)
                     /locus_tag="VIV63_03535"
     CDS             complement(787..1536)
                     /locus_tag="VIV63_03535"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY6123697.1"
                     /translation="MSERLQKLLAGAGHGSRRGIEEWIRAGRVTLNDRVAVLGDRATS
                     ADRICLDGQPLDLGGSIESVEVLLYHKPVGEVTTRSDPEDRPTVFDRLPSPASGRWIV
                     VGRLDVNTSGLLLFTNDGALAHRLMHPSSEIRREYLVRLRGTPPAEVLERLRTGVELE
                     DGKANFDEIHAESTEGSHSWLRVCLHEGRNREVRRLFEHEGFVVSRLSRLRYGSVELP
                     RDLRGGAFKSLLPAEIAELSALAFAGPVAAG"
     gene            1624..2565
                     /gene="cysK"
                     /locus_tag="VIV63_03540"
     CDS             1624..2565
                     /gene="cysK"
                     /locus_tag="VIV63_03540"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY6123698.1"
                     /translation="MIYDNILGTIGRTPIVRINRLAPAGTTIYVKCEFFNPLSSVKDR
                     LAIAIIEDAERSGKLKPGQTVVEATSGNTGIALAMVCAAKGYPFVAVMVETFSIERRK
                     IMRMLGAKVILTPAAERGQGMVRKAAELAQKQGWFLASQFDNPANPAYHRNTTGPEII
                     QDFVGKRLDNFVTGWGTGGTLTGVGEVLKLTRPDVKIIATEPEAAQLLGGKPFAPHKI
                     QGWTPDFVPAVLNKNVPDRIVPVTDVEAIEAARALARSEGIFVGISSGGTFAAALKVA
                     REAGPGSVTLAMLPDTGERYLSTALFEGISDGGDPEP"
BASE COUNT          475 a          866 c          817 g          446 t
ORIGIN      
        1 aatggtggag ggggaaggat tcgaaccttc gaaggcgtaa gccggcagat ttacagtctg
       61 ctcccgttgg ccgcttgggt acccctcccg ggaaaaacga gccgcgtatt ctcgtttcga
      121 ccccccggag tgtcaacggc attggcgcgt attttgctgt ggaatttggg cctgtgacct
      181 ttcaccccac cggcccggat ggcggggaca catctatata gggcaaagcc ggggtatcaa
      241 tggccagccg gggccccgct tgcgaaggag cgcaatcttc cgagctgggt acccatgacc
      301 tggtcgacga gcggtgccag tttggccgaa tccatcgtca acgcgccggc tacccgataa
      361 gtcatcttta ttcgggtccc cggtccgtcc ggggccaggg tgaaggtcag aaccgcgctc
      421 accgccatgt cctggagcgg tccgagcgcc ccttcgagcc tcagcatctt gccgggctgg
      481 gcaaaaatca cgcgcgcgtg ctcgactgag ccgttgtgag ccagcttttc gcagaagcag
      541 ccgccggctt ctggccagag tctgaggttg gccgcggagc ccgaccaggt atgcgcgggg
      601 tcccaccagc cagcgacctt gatgagtttg cgatacaccg cggcgggctt ggcctccgcc
      661 atcaccgtgg tctcgatcgt gaacccgttt tcccgggttt cacgaacctc cgcggcaacc
      721 ggtccggcga cgaccagggc tccgaggaac gacagtggca ggtgattttt catggtccgg
      781 aggctatcac ccggccgcca ccggccccgc gaacgcgagg gcggagagct cggcgatctc
      841 cgccggaagc agcgatttga aagcgccacc gcgtaaatct ctaggcaatt caacgctacc
      901 gtaccgcagg cggctcaatc ggctgaccac gaaaccttcg tgttcgaaca gccggcggac
      961 ctcccggttg cgtccttcgt gcaggcagac gcggagccag ctatgactgc cctcggtgga
     1021 ctcggcatgg atctcgtcga aattggcctt gccgtcttca agctcgacac cggttcgcag
     1081 ccgttccagg acctccgccg gtggcgtgcc gcgcaggcgc accaggtact cgcgacggat
     1141 ctcgctcgag gggtgcatca gccgatgggc gagcgccccg tcattggtga acagcaggag
     1201 tcccgaggtg ttcacgtcca gccggccgac gacgatccac cgtccgctgg cgggtgacgg
     1261 cagccggtcg aagacggtcg gccggtcttc cgggtcgctg cgcgtggtca cctcgccgac
     1321 aggcttgtgg tacagcagca cttccacgga ttcgatggac ccacccaggt cgagcggctg
     1381 tccatcgagg cagatgcggt cggcgctggt ggcgcgatcg cccagcacgg cgacacggtc
     1441 attgagggtc acgcggccgg cgcggatcca ctcttcgatg ccacggcgag aaccgtggcc
     1501 cgcgcccgcg agaagttttt gcaatcgttc gctcatgagg gcagcagcat aaccaactgt
     1561 tatatggcta ttccaaggcc agcctcgtct cgcctcaggg acccgcctag agtcgcccgc
     1621 accatgatct acgacaacat tctcggcact atcggccgta ctcccatcgt ccgcatcaac
     1681 cgtctcgcgc ccgcgggcac gacgatctac gtgaagtgcg aattcttcaa tccgctctcg
     1741 tcggtcaagg atcgactggc catcgccatc atcgaagacg cggagcgcag cggaaagctg
     1801 aagcccggcc agacggtcgt ggaagccacg tccggcaaca ccggcatcgc actcgccatg
     1861 gtttgcgccg cgaagggtta tcccttcgtc gccgtgatgg tcgaaacctt ctcgatcgag
     1921 cgccgcaaga tcatgcgcat gctgggcgca aaggtcatcc tcacgcccgc cgccgagcgc
     1981 ggccagggca tggtgcgcaa ggctgccgag ctcgcgcaga agcaaggctg gttcctcgcg
     2041 agccagttcg acaatccggc gaatcccgcc taccaccgca acaccaccgg tccggaaatc
     2101 atccaggact tcgttggcaa gcgtctcgac aacttcgtca ccggttgggg cacgggcggc
     2161 acactcaccg gcgtcggtga agtactcaag ctcacgcgcc cggacgtgaa gatcatcgcc
     2221 accgagccgg aagccgcaca gttgttaggc ggcaagccgt tcgcgccgca caagatccag
     2281 ggctggacgc cggacttcgt gcccgcggtg ctcaacaaga acgttccgga ccgcatcgtc
     2341 cccgtcacgg acgtcgaggc catcgaagcg gcgcgcgcgc tcgcgcgcag cgaaggcatt
     2401 ttcgtcggca tctcgagcgg cggcacgttc gccgcggcat tgaaggtcgc gcgcgaagcg
     2461 ggcccgggct cggtcacgct ggccatgctg cccgacaccg gcgaacgcta tctatctact
     2521 gcgctgttcg agggaatctc cgacggcggc gaccccgagc catgagcgga caaccgcgcg
     2581 aaacattcgg tcatgccgaa tcga
//
LOCUS       DATDEU010000140         5677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_316218, whole genome shotgun sequence.
ACCESSION   DATDEU010000140 DATDEU010000000
VERSION     DATDEU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316218"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VIV63_03545"
     CDS             complement(<1..401)
                     /locus_tag="VIV63_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123699.1"
                     /translation="MSNDNTQPSRNETITTATSGSQIREGVLAVPGELKLHFGGALES
                     VSIAWRLAGPAGAPVVVALGGISASRRVWLPDEPRGGWWHEVVGPGLALDTTRFRVLS
                     FDYLGASADSTGPRDDAPFPTVSAYDQAELLL"
     regulatory      complement(499..620)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            728..1039
                     /locus_tag="VIV63_03550"
     CDS             728..1039
                     /locus_tag="VIV63_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEY6123700.1"
                     /translation="MKQHRNVTARQEALSERSLFAAVLTLKTVDECRAFFRDLCTPAE
                     LQAIADRWAVVDWLQKGVPYREIHRLTGVSVTTIGRVARCLADGNGGYSLAVNRLQRH
                     A"
     gene            1093..1998
                     /gene="hisG"
                     /locus_tag="VIV63_03555"
     CDS             1093..1998
                     /gene="hisG"
                     /locus_tag="VIV63_03555"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY6123701.1"
                     /translation="MKPIDKDRLKLAVQKSGRLTDPSLELLKSCGLKLSRGRDQLMGF
                     GENMPLDVLFVRDDDIPDLVQEDVCDLGLVGRNVLEEKRLELISRGHAATFTELRTLD
                     FGRCRLSLAVPDGFSYEGPRSIAGKRIATTYPFTLERYLRERNITADIVTLSGAVEIA
                     PRLGRADLICDLVSTGSTLQANHLREVETVLESQSLLIRTPVALAPAKQEWVERLLLR
                     IDGVQQVRESKYIMLHAPRSALPEIRRLLPGSESPTIIPLEGSNDKVAIHAVCRENVF
                     WETLEGLKRAGASSLLVLPVEKMLA"
     gene            1998..3308
                     /gene="hisD"
                     /locus_tag="VIV63_03560"
     CDS             1998..3308
                     /gene="hisD"
                     /locus_tag="VIV63_03560"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY6123702.1"
                     /translation="MKLIDWTTSSPDARRAALSRPAADSRDEVFRQAASIITAVRAEG
                     DAAVRRFTAQFGGASSGELRASAAEFREARSTLTPDQIAALERAVANVARFHEAQMSA
                     PLSVETMPGVRCERITRPIDSVGLYVPAGSAPLPSTAIMLAVPARIAGCNARAIASSP
                     GPDGKVHAAVLVAAELCGIDTVYKMGGAQAIAALAFGTESVRKVDKIFGPGSAWVTAA
                     KQIVAADPAGAAIDLPAGPSEVLVIADESANAKFVAADLLAQAEHDTIAQVILVTDSE
                     RLAQRVVVELAEQACTLSRRDIIFQSMASSRCIIVKDLAAAIDVSNEYAPEHLIIQTR
                     DPRAMLASVSCAGSVFLGEWSPESMGDYCSGTNHVLPTFGYARSYSGVSLVDYQKRIT
                     VQELSADGLRALGPTAVTLSGMEGLDAHGNAVKVRLDALSGGAA"
     gene            3305..4354
                     /gene="hisC"
                     /locus_tag="VIV63_03565"
     CDS             3305..4354
                     /gene="hisC"
                     /locus_tag="VIV63_03565"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY6123703.1"
                     /translation="MTSILALARPDIVALKPYSHASWDPAFERLHANELPWRAETDRS
                     VAGLNRYPEPHPHVLALRLAQLYGVSVEHLLPGRGSDESIDLLVRGFCRAGVDNVVLC
                     PPTFGMYAVAARIQGAEVREVPLVAERGFALDTAGVLAACDAHTKIVFLCSPNNPTGN
                     AMAPAAIEQLLVALSARSLVVVDEAYIEFSGEASLTSALARFPNLVVLRTLSKAFGLA
                     GARVGSLIAAPEIVALLSKVIPPYSIPQLTIEAVLATLAEPQLAIARERVAQIRAERE
                     RVRAALANCKGVQKIWPSVANFLLVDFADAAAVLDAARGAKLLIRDMRNVSPRSLRIS
                     VGSPEQNDRLIRSLA"
     gene            4351..5421
                     /gene="hisB"
                     /locus_tag="VIV63_03570"
     CDS             4351..5421
                     /gene="hisB"
                     /locus_tag="VIV63_03570"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HEY6123704.1"
                     /translation="MSKTRVLFVDRDGTLIEEPPDEQVDSIEKIRFMPDVFAAMREAQ
                     KAGFKLAMVTNQDGIGSETFPQKAFDVPHQFMMDAFSSQGVEFDAVFVCPHRKSDGCD
                     CRKPKTKLVDDWVREREVDLAGSAMIGDRETDMEFARNLGIRGLMVRRHGNKWETWPA
                     ILRELTERRATIRRKTKETDIHVTVNLDTTAPITIATGIGFFDHMLEQLAKHGGFSLE
                     LKCHGDLEIDEHHTVEDCALAVGEALREALGAKLGLARYGFMLPMDEAQVRVAIDLSG
                     RAYGVFEGKFAREAVGGLPTELVPHFFKSLAESLKAAIHVTIVGENSHHMIEACFKGV
                     GRALRMALRREGEELPSTKGVL"
     gene            5418..>5677
                     /gene="hisH"
                     /locus_tag="VIV63_03575"
     CDS             5418..>5677
                     /gene="hisH"
                     /locus_tag="VIV63_03575"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY6123705.1"
                     /translation="MSVPVAIIDSGGANIASLRAALSRLGAESVVTSDHGVIQRATRV
                     LLPGVGSAHNAMARLRGTGLDKLIPTLKQPLLGICLGMQILF"
BASE COUNT          985 a         1917 c         1786 g          989 t
ORIGIN      
        1 agcagtagtt cggcctggtc gtaggccgac accgttggga acggcgcgtc gtcgcgcgga
       61 ccggtggaat cggcgctggc gccgagatag tcgaagctca ggacgcggaa gcgcgtcgtg
      121 tccaacgcga ggccgggccc cacgacttcg tgccaccagc cgccgcgggg ctcgtcgggc
      181 aaccagacgc ggcgcgaagc cgagatgccg ccgagcgcga cgaccaccgg tgcgcccgcg
      241 ggacccgcga gccgccaggc aatcgagacc gattcgagtg cgccaccgaa atgcagcttg
      301 agttcgccgg gcaccgcgag cacgccttcg cggatctgcg aaccggacgt ggccgtggtg
      361 atcgtttcgt tgcgggaagg ctgtgtgttg tcgttgctca tccagatgct ccaaagctgt
      421 actcagcggg gcactcgaga gcagccgcgc gatatgcgcg gcacctgcgc tgcccggata
      481 tgccgggcgg cggaaggctg ctcatctgcc ggacccagat cttgttaaag agtggttccg
      541 caggaagtgg cacctttccg catttgcggc ggttgccccg gcgtcttcgg gccttatccc
      601 tcagccggtc tcgatgagtg cgcgaattct atgaggcggt ttcaccacaa gcaagcgcgg
      661 agcttgcatt tgtgcggtgt cacgggtcta atgcgccacc gtattaacgc gctattacat
      721 taattcaatg aaacagcatc ggaatgtcac tgcccgccag gaggcgcttt cggagcgaag
      781 cctgttcgcc gcggtgctga cgctgaagac ggtggacgag tgccgcgcgt ttttccgcga
      841 cctctgcacg cccgcggagc ttcaggccat tgccgaccgc tgggcggtcg tggactggct
      901 gcagaaaggc gtgccctatc gtgagatcca tcgcctcacc ggcgtcagcg tgaccaccat
      961 cgggcgcgtt gcgcgttgcc tcgcggatgg caatggcggc tattcgctgg ccgtgaaccg
     1021 tctgcagcgc cacgcctgag cccctttcgc ccgggagatc tcccgggctc acctcgcatc
     1081 caccgaaaac ctatgaaacc catcgacaaa gatcgcctca aactggccgt gcagaaatcg
     1141 ggacggctca ccgatccatc gctcgagctg ctcaagagct gcggcctcaa gctttcgcgc
     1201 ggacgcgatc agctcatggg tttcggcgag aacatgccgc tcgacgtcct gttcgtgcgc
     1261 gacgacgaca ttcccgatct cgtgcaggaa gacgtctgcg atctcggcct cgtcggacgc
     1321 aacgtgctcg aagagaagcg tctcgagctc atctcgcgcg gtcatgccgc caccttcacc
     1381 gaacttcgca cgctcgactt cggccgctgc cgattgtcgc tggccgtgcc ggacgggttc
     1441 agctacgaag gtccgcgttc tatcgccggc aaacgtatcg ccaccaccta tccgttcacg
     1501 ctcgagcgtt acctgcgcga gcgcaacatc accgccgaca tcgtcacgtt gtccggcgcg
     1561 gtggaaatcg cgccgcgcct cggccgcgcc gatctcatct gcgacctcgt gtcgacgggc
     1621 tcgaccttgc aggccaacca cctgcgcgaa gtggagaccg tgctcgaaag ccagtcactg
     1681 ctcattcgca cacccgtcgc gctcgcgccc gccaaacagg aatgggtcga acgcctgttg
     1741 ctgcgtatcg acggcgttca gcaggtgcgt gaaagcaagt acatcatgct gcacgcgccg
     1801 cgctcggcgc tgccggagat tcgccggctg cttccgggca gcgaatcgcc caccatcatt
     1861 ccgctcgaag gctcgaacga caaagtcgcg attcacgccg tgtgccgcga gaacgtgttc
     1921 tgggaaacgc tcgaaggcct caagcgcgcc ggcgcgagtt cactgctcgt tcttcccgtc
     1981 gagaaaatgc tggcctgatg aaactcatcg actggaccac ttcgtcaccg gatgcgcgtc
     2041 gcgcggcgct gtcgcgcccg gccgccgaca gccgcgatga agtcttccgc caggccgcga
     2101 gcatcatcac cgcggtgcgc gccgaaggcg acgcggccgt gcgccggttc accgcgcagt
     2161 tcggcggtgc ctcatcaggc gagttgcgcg ccagcgcggc ggagttccgc gaagcacgct
     2221 ccacgctgac tcccgatcag atcgccgcgc tcgaacgcgc ggttgccaac gttgcgcgtt
     2281 tccacgaggc gcagatgtcg gcgccgttgt ccgtcgagac catgcccggc gttcgctgcg
     2341 aacgcatcac gcgccctatc gacagcgtcg gcctttacgt tcccgcgggc tctgccccat
     2401 tgccttcgac cgccatcatg ctcgcggtgc cggcccgcat cgccggctgc aacgcgcgcg
     2461 cgatcgccag ttccccgggg cccgatggca aggtgcacgc ggcggtactc gtggccgcgg
     2521 aactctgtgg catcgacacc gtgtacaaga tgggtggcgc acaggcgatc gcggcgctcg
     2581 ccttcggcac cgagagcgtt cgcaaggtcg acaagatatt cgggcctggc agcgcctggg
     2641 tgaccgcggc caaacaaatc gtcgccgcgg accctgctgg cgccgccatt gacctgcccg
     2701 ccggtccgtc cgaagtgctg gtgatcgccg acgaatccgc caatgcgaaa ttcgtcgccg
     2761 ccgatctcct tgcgcaagcg gaacacgaca ccatcgcgca ggtgatcctc gtcaccgact
     2821 ccgagaggct cgcgcagcgg gtcgtcgtcg agctggccga acaggcgtgc acgctcagcc
     2881 gccgcgacat catctttcag tccatggcca gcagccgctg catcatcgtg aaagacctcg
     2941 ccgcggccat cgatgtgtcg aacgaatacg cgcccgaaca cctcatcatc cagacgcgtg
     3001 atccgcgcgc aatgctcgcg agcgtgagct gcgcgggctc ggtgttcctc ggtgagtggt
     3061 caccggaatc gatgggtgac tattgttcgg gcaccaacca cgtgttgccc actttcgggt
     3121 atgcgcgcag ctacagcgga gtttccctgg tcgactatca aaagcggatc accgtgcagg
     3181 aactcagtgc cgacggactg cgtgcgctcg gcccgaccgc cgtcacgctc tccggcatgg
     3241 aaggtctgga tgctcacggc aacgccgtga aagtccggct cgatgccttg tccggaggtg
     3301 cggcatgacc tccatcctcg cgctcgcgcg gcccgacatc gtcgcgctca agccctattc
     3361 gcacgcgagc tgggacccgg ctttcgagcg tctgcacgcc aacgaactac cgtggcgcgc
     3421 cgaaacggat cgctccgtcg ccgggctcaa tcgttatccc gagccgcatc cgcacgtact
     3481 ggcgctgcgg ctagcacagc tctacggcgt gtcggtcgaa cacctgttgc cgggccgcgg
     3541 cagtgacgaa tccatcgact tgctggtgcg cgggttctgc cgcgccggtg tcgacaacgt
     3601 cgtcctttgc ccgcctacct tcggcatgta cgcggtcgcc gcccgtatcc agggagcgga
     3661 ggttcgcgaa gtaccgttgg tggcggaacg cggctttgcg ctcgataccg ccggcgtgct
     3721 ggcggcgtgc gacgcccaca cgaagatcgt gttcctgtgc agccctaaca atcccacggg
     3781 caatgcgatg gccccggcgg ccatcgagca attgctggtg gcgctgtccg cgcgatcgct
     3841 cgtggtggtc gacgaggcat acatcgaatt ctccggcgaa gcatcgctga cgagcgcgct
     3901 ggcccggttt ccgaacctgg tggtgctgcg aaccttgtcg aaggcgttcg gcctcgcggg
     3961 tgcgcgagtc ggatcgctga ttgcggcgcc cgaaatcgtg gcactgctga gcaaggtgat
     4021 cccgccctat tccatccccc agctcaccat cgaggccgtg ctcgcgacgc tcgccgagcc
     4081 gcaactcgcc attgcgcgtg agcgcgtggc gcagatccga gccgaacgcg aacgggtacg
     4141 cgcggcgctt gcaaactgca aaggcgtgca gaagatatgg cccagcgtgg ccaacttcct
     4201 gctcgtcgat ttcgcggatg cggccgccgt gctcgatgcg gcgcgcggcg ccaaactgct
     4261 cattcgcgac atgcgcaacg tttcgccgcg ttcgcttcgc atatccgtgg gtagtcctga
     4321 acagaacgac agattgatcc ggagcctggc atgagcaaga cccgcgtgct gttcgtcgac
     4381 cgcgacggca ccctcatcga ggagccgcct gacgaacagg tcgactccat cgaaaagatc
     4441 cgcttcatgc ccgacgtgtt cgccgccatg cgggaagcgc agaaggccgg cttcaaactc
     4501 gccatggtca cgaaccagga tggcatcggc agcgaaacct tcccgcagaa ggccttcgac
     4561 gtgccgcacc agttcatgat ggatgcgttc agctcgcagg gtgtcgaatt cgacgccgtg
     4621 ttcgtctgcc cgcatcgcaa gagcgacggc tgcgactgcc gcaagccgaa gaccaaactc
     4681 gtggacgact gggtgcgtga acgcgaagtg gacctcgccg gcagcgcgat gatcggagat
     4741 cgtgaaaccg acatggagtt cgcgcgcaac ctcggcattc gcggactcat ggtccgccgt
     4801 catggcaaca agtgggaaac ctggcctgcc atcctgcgtg agctcacgga acgtcgcgcg
     4861 acgatccgcc gcaagacgaa ggagaccgac atccacgtca cggtgaatct cgacacgacc
     4921 gcgccgatca ccatcgcgac cggcatcggc tttttcgacc acatgctcga acagctcgcc
     4981 aagcacggcg gcttctcgct cgagctcaag tgccatggag atctcgagat cgacgaacat
     5041 cacaccgtcg aagactgtgc tctcgcggtg ggcgaggcat tgcgcgaagc gctcggcgcg
     5101 aagctcggcc tcgcacgtta cgggttcatg ctgcccatgg acgaagcgca ggtgcgcgtc
     5161 gcgatcgacc tctcaggccg cgcgtatggc gtattcgaag gcaagttcgc gcgcgaagcc
     5221 gtgggcggac tgcccaccga actcgtgccg cacttcttca agtcgctcgc ggaaagcctc
     5281 aaggccgcca ttcacgtcac catcgtcggc gagaactcgc accacatgat cgaagcctgc
     5341 ttcaagggcg tcggccgcgc gttgcgcatg gcgctgcgcc gcgagggcga agagctgccg
     5401 tcgaccaagg gcgtgttgtg agcgttccgg tcgccatcat tgatagtggc ggggccaaca
     5461 tcgcctcgct gcgcgccgcg ttgtcgcgcc tcggcgcgga atccgtcgtc accagcgacc
     5521 acggcgtcat ccagcgcgcc acgcgcgtgt tgcttccggg cgtcggctcg gcgcacaacg
     5581 ccatggcgcg cctgcgtggc accggcctcg acaagctgat cccgacactc aagcagccct
     5641 tgttaggcat ctgcctcggc atgcagatcc tgttcga
//
LOCUS       DATDEU010000141         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1191949, whole genome shotgun sequence.
ACCESSION   DATDEU010000141 DATDEU010000000
VERSION     DATDEU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191949"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /gene="greB"
                     /locus_tag="VIV63_03580"
     CDS             complement(<1..428)
                     /gene="greB"
                     /locus_tag="VIV63_03580"
                     /inference="COORDINATES: protein motif:HMM:NF002506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY6123706.1"
                     /translation="MTRYRPTEKPGSQFITAAGAARLKQELDELWKVERPRVTQAVSE
                     AAAQGDRSENAEYTYGKRRLREIDRRVRFLRKRLDGMTVVEKPPSDPKRVFFGAWVTL
                     EDDDGKQTKYRIVGPDEFDMGDGYISMDAPLARALMRKAL"
     gene            539..733
                     /locus_tag="VIV63_03585"
     CDS             539..733
                     /locus_tag="VIV63_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123707.1"
                     /translation="MAARYYTHFVMSAEPAHSSEWSGVIELTHPLGERRDSKELRRLL
                     AANFEIDAEDIKILQVARLH"
     gene            866..>2571
                     /locus_tag="VIV63_03590"
     CDS             866..>2571
                     /locus_tag="VIV63_03590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY6123708.1"
                     /translation="MDRLVVMASVAVLAGCAASTPKPATPPAAPAVAPLKSGLDLTGF
                     DRRVRPQDDLYRFAGGAWLANTPIPADRSNYGSFIILDDQAQEEVKQLIVAASTQANR
                     PTGSDAQKVGDFYLSYMDTARVESLGLAPLKEELARIDAITTARDVARYIGYAQRIGV
                     AQPFAWFSSPDNGNSTVYLGALYQNGLTMPDRDYYLSPDEKYATFRAKFAEYVEQMLA
                     RAGERNARSAAARIAALETRIANYQWTKVQNRDPVKTYNPMTLPEYQKLAPGFDWLAF
                     FEGMGAPVQRLDVNQPSFIKGMAQLVKTVPVSDWRVYFKFQLLDDYAPALSAQFADLE
                     FGFHQKTIAGVPEPRPRWRRAVDSMDNNMGELIGRMFVESHFGADAKASMLELVGNLL
                     KAFDSSIDGLEWMSPQTRAEAKKKLAKITVKIGYPDKWRDYSLLSVRNDDLVGNLLRA
                     AAFQHNRLVTRTGGPVDKGEWLMTPQTVNAYYNPTTNEITFPAAILRWPFFDKSADPA
                     VNYGGIGSVIGHEISHGFDDSGRQYDGDGNLRDWWTAEDGAKFKQRAGELVKQFAGYT
                     VL"
BASE COUNT          469 a          824 c          785 g          493 t
ORIGIN      
        1 tcgagcgcct tgcgcatgag tgcgcgggcc agtggcgcgt ccatgctgat gtagccgtca
       61 cccatgtcga attcgtcggg cccgacgatc cggtactttg tttgtttgcc gtcgtcatcc
      121 tcgagcgtca cccaggcgcc gaaaaacacg cgcttcggat ccgagggtgg cttctccacc
      181 acggtcatgc cgtcgaggcg ttttcgcaga aaacgcacgc gccggtctat ttcgcgcagg
      241 cggcgttttc cgtaggtgta ttcagcattc tcggagcgat cgccctgcgc cgcggcctcg
      301 gaaacggcct gggtgacgcg cgggcgttcg actttccaca gctcatccag ctcttgtttg
      361 aggcgtgctg cacccgcggc ggtgatgaat tgcgatccgg gtttctctgt aggtcggtaa
      421 cgagtcaagg cttttaaatc atatgtgatg ggtgtctcag tcacggcaca ccctggccgg
      481 ggggttctgt gcagcggctc acagccgttt cggggtagcc aatctacgct ttctacccat
      541 ggcagcccgt tactacacgc actttgtcat gagcgcagaa cccgcgcatt cgagcgagtg
      601 gagcggggtc atcgagctga cgcacccatt gggcgaacgc cgggattcga aggaactgcg
      661 ccggctgctg gccgcgaact tcgagatcga cgccgaggac atcaagattt tgcaggtagc
      721 acgcctgcac tgacgatttt ttgctgaatc ccctagcgga cttctttccc ttcatcgaaa
      781 gtccgccttc tgaagccctg agccccaaaa gctcggggct ttttttatac tcggccccat
      841 cgccttttca tcccggggtt ttcgtatgga tcgtctcgtc gtcatggctt cggtggccgt
      901 gcttgccggc tgcgccgcct caacaccgaa gcccgcgaca ccacccgccg cacccgccgt
      961 ggcgccgctc aaatccggac tcgacctcac gggcttcgac cgccgcgttc gtccgcagga
     1021 cgacctctac cggttcgccg gcggcgcctg gctggcgaat acgccgatac ccgcggatcg
     1081 atccaattac ggctcgttca tcatcctcga cgaccaggca caggaagagg tgaagcagct
     1141 catcgtcgcg gcgtccacgc aggcgaatcg accgaccggc tccgatgcgc agaaggtcgg
     1201 cgacttctat ctctcataca tggacaccgc gagggtcgag agcctggggc tcgctccgct
     1261 gaaagaggaa ctggcgcgta tcgacgccat caccaccgcg cgcgacgtgg cccgatacat
     1321 cggctatgcg caacgcattg gagtcgcgca gccgttcgcc tggttttcgt cgcccgacaa
     1381 cgggaactcc acggtctacc tgggcgcgct gtaccagaac gggctcacca tgcccgaccg
     1441 cgattactac ctctctcccg atgaaaagta cgcgaccttc cgcgcgaagt tcgccgagta
     1501 cgtcgagcag atgctggcgc gcgccggcga gcgtaatgcc cggtccgcgg cggcgcgcat
     1561 cgcggcgctc gagacccgca tcgccaacta ccagtggacc aaggttcaga accgcgatcc
     1621 ggtgaagact tacaacccga tgacattgcc ggaatatcag aagctcgcgc cgggcttcga
     1681 ctggctcgcg ttcttcgaag gcatgggcgc gcccgtgcag cggctcgacg tgaaccagcc
     1741 gtcgttcatc aagggaatgg ctcagctcgt gaagacggtg ccggtgtccg actggcgcgt
     1801 ctatttcaag ttccagcttc tcgacgacta cgcaccggct ctgtcggcgc aattcgccga
     1861 tctcgaattc ggtttccacc agaaaacgat cgcaggcgtg ccggagcccc ggccgcgctg
     1921 gcggcgcgcg gtggattcca tggacaacaa catgggcgag ctcatcggcc gcatgttcgt
     1981 cgagtcgcat ttcggcgcgg atgcgaaggc cagcatgctc gagctggtgg gcaacctgct
     2041 caaggctttc gacagttcga tcgacggcct cgagtggatg agcccgcaga cgcgtgccga
     2101 agcgaagaag aagttggcga agatcaccgt gaagatcggt taccccgaca agtggcgcga
     2161 ctacagcctg ctgtccgtgc gcaacgacga tctcgtcggc aacctgcttc gggccgccgc
     2221 tttccagcac aaccggttgg tgacccgcac gggcgggccc gtggacaagg gcgagtggct
     2281 gatgacgccg cagaccgtca acgcgtatta caacccgacc accaacgaga tcacgttccc
     2341 ggccgccatc ctgcgctggc cgttcttcga caagtctgcc gacccggccg tgaactacgg
     2401 cggcatcggt tccgtgattg gtcacgagat cagtcacggc ttcgacgatt ccggccgtca
     2461 gtacgacggc gacgggaatt tgcgcgactg gtggacggcc gaagacggcg cgaaattcaa
     2521 gcaacgggcc ggtgagctgg tgaagcagtt cgccggttac acggtattgg a
//
LOCUS       DATDEU010000142         4428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_316306, whole genome shotgun sequence.
ACCESSION   DATDEU010000142 DATDEU010000000
VERSION     DATDEU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316306"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="VIV63_03595"
     CDS             <1..1659
                     /locus_tag="VIV63_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123709.1"
                     /translation="MQRFGLAMDAAVNGAGQIVCRSQFDPAAAFAYDDTVQLSVDTLA
                     ADVAACAPLNPFGQGNISQAARDFVLQDTTSVGEIKQQVVSATLTGDSSALFSLPAGP
                     IGIALGFEHRTEENFFQADELVSSGITFYNALPLFDPQKFKVSEVFTELRVPLLKELP
                     FAEELTLNLAGRFADYDGNTGEVFAYNAGIDWAPISSLRFRAGTARAVRAPSLVDLYS
                     EQSQNFATINDPCSYRNVGAGASTRVANCAAAGIPGGPPASGGYDHVYTVTPEILSGG
                     NPELTEETSDSLTIGFVFQPEFAPGLSFSADYFDIEIDDVITAPTAQQIMNACYDASD
                     IDNQFCGLFQRWGAAGGPDFDGSPNHPESPYALVNGTLQQTLLNYASSTAKGIDFELG
                     YNHSAGESGTVKMRLLYTLMLERDDFLDPSNPVDADQTLLELGDPKDAFNFNVDYEVG
                     ELTLGYQLRYIGKQVLFNYEDTYGLQGEPPQNADYADVRFYPSVMIQDIRLAYDINEE
                     WNAYVGCDNFADVLPPLGLTGATEGGGIYDPRGRYFYAGVKVSF"
     gene            complement(1804..2523)
                     /locus_tag="VIV63_03600"
     CDS             complement(1804..2523)
                     /locus_tag="VIV63_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6123710.1"
                     /translation="MQMFGSLLFNVFLYAWMLVFAFTYCVVCPFLSFRGRVWMAGIMT
                     RVVFFAARVLCGIRFSVEGRENLPEGNHVIFMKHSSTWETFGIMILFPPLVWVMKREI
                     LWIPFVGWGCILASCIAIDRKAGASAVNQVLAQGKERLERGAWILVFPEGTRMAAGET
                     RRYGSSGTLLAAQENRKIVPVAHNAGYFWPRRGVLKKRGTIRVIIGPPVDPVGRDVRE
                     VNNEVQAWIESHVIPPADTHV"
     gene            complement(2544..>4428)
                     /gene="glyS"
                     /locus_tag="VIV63_03605"
     CDS             complement(2544..>4428)
                     /gene="glyS"
                     /locus_tag="VIV63_03605"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY6123711.1"
                     /translation="AQAIKLKGPPVSAAFDKDGRPTAAATKFAEKCGADVTALTRVTE
                     GKGEFLYFAGSKPGLATAGLLPAIVQKSLDSLPIPKRMRWGSSSAEFVRPVHWLVMLF
                     GADVIPARILDTDAGRATRGHRFMAPQEFALASPSDYETVLREKGKVIPDFGERRLRI
                     GAQVTAAAKSLSGEALLSDSLLDEVTALVEWPTAIAGSFEPRFLELPREVLISTLQQH
                     QRYFPVQSGAKLLPHFITVSNIESLEPAKVRAGNERVVRPRLSDAAFFWSQDRKQALA
                     ARLPGLDAVTFQAKLGSIGDKVRRVVTLSGEIALLIDANREKAERAANLAKCDLLSSM
                     VGEFPELQGIMGRYYAVADGEADDVAAAIDEHYLPRGAGGALPATGAGVAVALADKLD
                     TLAGIFAIGQKPSGTKDPFGLRRAAIGALRILVEKRVDLDLRALVTRACALQPVQNPA
                     AATEVWDYMVERLRAYFLDSGSAAGVASVTTEMFDAVRASAPVSPLDFAARLSALGNF
                     LGLAEAGSLTAANKRIANILKKAETGTGTVDVSALLEPAEKQLHEALAAMVPEVERAV
                     AKRDYAAALTRLATLRPVVDGFFDGVMVNAEDPALRRNRLALLAQVRHQFTRIADLSC
                     LPG"
BASE COUNT          807 a         1453 c         1420 g          748 t
ORIGIN      
        1 atgcagcgct tcggtctcgc catggacgcg gcggtcaacg gcgcgggcca gatcgtctgc
       61 cgctcgcagt tcgacccggc agcggctttc gcctatgacg acaccgtaca gctgtcggtc
      121 gacacgcttg ccgccgacgt cgcggcttgc gcgccgctga atccgttcgg ccagggcaac
      181 atctcgcagg cggcacgtga cttcgtgttg caggacacga cctcggtcgg cgagatcaag
      241 cagcaggtag ttagcgcaac gctgaccggc gactccagcg cgttgttctc gctgcccgcc
      301 ggcccgatcg gtatcgcgct cggcttcgag catcgcaccg aagagaactt cttccaggcc
      361 gatgagctgg tgtcgagtgg catcacgttc tataacgcgc tgccgctgtt cgacccgcag
      421 aagttcaagg tgagcgaagt gttcaccgag ttgcgcgtac cgctgctcaa ggaactgccg
      481 ttcgccgaag agctgacgct gaacctggcc ggccgtttcg ccgactacga cggcaacacg
      541 ggtgaagtgt tcgcctacaa cgcgggcatc gactgggcgc cgatctcgag cctccggttc
      601 cgcgctggca cggcacgcgc agtgcgtgcg ccgagcctcg tggacctgta ctccgagcag
      661 agccagaact tcgcgaccat caacgatccc tgctcctacc gcaacgtggg cgcgggtgcg
      721 tcgactcgcg tggcgaactg cgcggccgcg ggtattcccg gcggtccgcc ggcgtcgggt
      781 ggctacgatc acgtctacac ggtgacgccc gaaatcctga gcggtggcaa tccggaactc
      841 accgaagaga cttcggattc gctcacgatt ggcttcgtat tccagccgga gttcgcgcca
      901 ggcctgtcgt tctcggctga ctacttcgac atcgagatcg atgacgtgat cacggcgccg
      961 acggcgcaac agatcatgaa tgcctgctac gacgcttcgg acatcgacaa ccagttctgc
     1021 ggactgttcc agcgctgggg cgcggcggga ggcccggact tcgacggctc gccgaatcac
     1081 cccgagtccc cgtacgcact ggtcaatgga acgttgcagc agacgttgct gaactacgcg
     1141 agctcgacgg caaagggtat cgacttcgag ttgggctaca accactcggc gggcgaatcc
     1201 ggaaccgtca agatgcgcct gctctacacg ctgatgctcg aacgtgacga tttcctggat
     1261 ccgtcgaatc cggtcgatgc ggatcagacg ctgctggaac tcggcgatcc gaaggacgcg
     1321 ttcaacttca acgtggacta cgaagtgggc gagctgacgc tcggctacca gctccgctac
     1381 atcggcaagc aggtgctctt caactatgaa gatacctacg ggctgcaggg cgagccgccg
     1441 cagaacgcgg attacgccga tgtccgcttc tatccgagcg tgatgattca ggacatccgc
     1501 ctcgcctacg acatcaacga agagtggaat gcctacgtgg gctgcgacaa cttcgccgac
     1561 gtacttccgc cgttggggtt gaccggcgca acggaaggcg gcggcatcta tgacccgcgc
     1621 ggccgctact tctacgctgg cgtgaaggtg tccttctgag atgaagccgg gccggcaggc
     1681 gactgccggc ccgacgtgat tgccagagag ttactcgacc ttcgcggcaa acaggcctgc
     1741 cgcctcgccg cttgggtcaa agggccgggt taatacccgg cccctttttt tgtgcgccag
     1801 ctttcagacg tgggtgtcgg ccggcggaat cacgtggctc tcgatccacg cctgaacttc
     1861 gttgttaacc tcgcgcacgt cgcgacccac cggatcgacc ggaggcccga tgatcacgcg
     1921 aatggtgccg cgcttcttga gcacgccgcg gcgcggccag aaataaccgg cgttgtgcgc
     1981 caccggcacg atcttccggt tttcctgcgc ggctaacaaa gtaccgctgg acccgtagcg
     2041 gcgcgtttca ccagccgcca tgcgagtgcc ctcgggaaac accaggatcc aggcgccgcg
     2101 ctcgaggcgt tccttgccct gtgccagcac ctggttcacc gccgatgcgc cggccttgcg
     2161 gtcgatggcg atgcagctcg cgagaatgca tccccaaccc acgaacggaa tccacaggat
     2221 ctcgcgcttc atgacccaca ccagcggcgg gaacaggatc atgattccga aggtctccca
     2281 cgtcgaagag tgcttcatga agatgacgtg attgccttcc ggaagatttt cgcgcccctc
     2341 gacggagaag cggatgccgc acaacacgcg cgcggcgaag aacacgacac gcgtcatgat
     2401 gccggccatc cagacgcggc cgcggaacga gaggaacggg cacaccacgc aataggtgaa
     2461 cgcgaacacc agcatccacg catacagaaa gacgttgaat agaagcgacc cgaacatctg
     2521 catgaatgtc ttccggcggc gactcagccc ggcaggcagg acagatcggc gatgcgcgtg
     2581 aactgatggc gcacctgcgc cagcagcgcc agacgattgc ggcgcaacgc cggatcttcg
     2641 gcgttgacca ttacaccgtc gaagaaaccg tcgaccacgg ggcgcagggt tgccaggcgc
     2701 gtgagcgcgg ctgcgtagtc gcgcttcgcc actgcgcgct cgacctccgg gaccatggcc
     2761 gccagcgctt cgtgcagctg cttttcagcg ggctcgagca gcgccgaaac gtccacggtg
     2821 cccgtgccgg tttcggcctt cttcaggatg ttcgcgatgc gcttgttggc cgcggtgagg
     2881 cttcccgctt cggcgagacc caggaaattc ccgagcgcgg aaagccgcgc ggcgaaatcg
     2941 agaggagaga cgggcgcgct ggcgcgcacg gcgtcgaaca tctcggtggt gacgctggcg
     3001 acacccgcag cacttccact gtcgagaaaa tacgcgcgca ggcgttccac catgtagtcc
     3061 cacacttcgg tcgcggccgc cgggttctgc accggctgca acgcgcacgc gcgcgtcacg
     3121 agcgcgcgaa gatcgagatc gacgcgcttc tcgacgagga tgcgaagcgc gccgatggct
     3181 gcgcgccgca gtccgaaggg gtccttggtg ccgctcggct tctggccgat cgcgaaaatt
     3241 ccagccagcg tatcgagctt gtccgcgagc gcgaccgcca caccggcgcc cgtcgccggc
     3301 agggcgcccc cggcgccgcg cggtagatag tgctcgtcga tcgcggccgc gacgtcgtcg
     3361 gcctctccat cggcaaccgc gtaataccgg cccatgattc cctgaagctc gggaaattct
     3421 ccgaccatcg acgacaggag gtcgcatttc gcaagatttg cggcacgctc cgctttttcg
     3481 cgattggcgt cgatgagcaa cgcgatctca cccgacagcg tgacgacacg gcgcaccttg
     3541 tcgccgatcg agccgagctt ggcctggaag gtgaccgcgt cgagtcccgg caggcgggcc
     3601 gcgagcgcct gcttgcgatc ctggctccag aagaaggcgg cgtcggacag gcgcgggcga
     3661 accacacgct cgttgccggc gcgcaccttg gcgggctcga ggctttcgat gttgctcacg
     3721 gtgatgaagt gcggcagtag tttggcgccg gactgcacgg ggaagtatcg ctggtgctgc
     3781 tgcagcgtgg agatgagcac ttcgcgcggc agctcgagga accggggctc gaacgaaccc
     3841 gcgatggcgg tgggccactc cacgagcgcg gtgacttcat cgagcagcga atcactgagc
     3901 aacgcctcgc cggacaggga cttcgcggcc gccgtgactt gcgcgccgat gcgcaggcgg
     3961 cgctcgccga agtccgggat cactttgcct ttctcgcgca gcacggtttc gtagtcggaa
     4021 ggagaggcga gcgcgaactc ctgcggcgcc atgaaacgat gcccgcgcgt cgcccggccg
     4081 gcatccgtgt cgagaatgcg cgcgggaatc acgtccgcac cgaacaacat caccagccag
     4141 tgcacggggc gaacgaactc cgcgctcgat gatccccagc gcatgcgctt ggggatgggc
     4201 agggaatcca gcgacttctg cacgatggcc ggcaacagcc cagccgtcgc gagacccggc
     4261 ttcgagcccg cgaaatacag gaactcgccc ttgccctcgg tcacgcgcgt gagggcggtg
     4321 acatccgcgc cgcacttctc cgcgaacttg gtcgcggccg ccgttggtct accgtccttg
     4381 tcgaacgcgg cactgaccgg tgggcccttg agcttgatgg cctgggcc
//
LOCUS       DATDEU010000143         5655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1775437, whole genome shotgun sequence.
ACCESSION   DATDEU010000143 DATDEU010000000
VERSION     DATDEU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775437"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VIV63_03610"
     CDS             complement(<1..631)
                     /locus_tag="VIV63_03610"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEY6123712.1"
                     /translation="MMIHVPKVLTHEQVAECRRLLDSAEWSDGKATVGEQGALVKKNR
                     QLPELSPVGRQLGELILTALSRSPLFFSAALPLKTVPPLFNRYEGGEQYGTHIDGAVR
                     AVPGSSHFIRTDLSSTLFLTDPSEYDGGELTVFDTFGQHDVKLPAGDLVLYTSGSLHR
                     VNPVTRGARVCSFFWTQSMVADERHREVLYRLDQTIQTLRGKHGESDETV"
     gene            complement(670..2931)
                     /locus_tag="VIV63_03615"
     CDS             complement(670..2931)
                     /locus_tag="VIV63_03615"
                     /inference="COORDINATES: protein motif:HMM:NF007349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HEY6123713.1"
                     /translation="MSNLSIAVAVSLALSAAAAKAQTATEEAKTMPKVSVAAPEPVET
                     PKVDRVESPKFTQPLLDTPQTIAVVSKEVLKQQGATTLSQALRNTPGVTFLLGENGNT
                     ATGDSIFMRGFDTQGSIFIDGIRDLGTVTRDTFNTEQVEIAKGPAGPDYGRGAASGYV
                     NMASKVPTVDSFTGGTASYGTASNARITGDINHRFEGSGTALRLNVMGQDGEVDGRDF
                     IERKGWAVAPSIAFGLEGDTRSYFYLLHTEQNNVPDGGVTTLGVDGFYNRDLDPNPDP
                     PLPLPRAPGVIAAPVDSENYYGYRSDFEETSGTMFTARFEHDFSDNVSLRNSTRYGRL
                     EQFYVLTGVNAVTIPSPTPDTWTVNRTRQSKFQENTLRTNQTNLTINVAAGGVSHAIT
                     TGVEFISEEQYNPAYAAASLGTLEPANPYEPGLTDAPPLYDPVRNGVYTRGETQTIGA
                     YLFDTASFAEKWEVTGGFRFDTYDTDFESMALTGTVPNQVLTPAQLGTDDTLFSYKVG
                     VLYKPLPNGSIYLSHANSKQPPGGANFTLTPPNGANNANRTDLEPTEGENLELGTKWE
                     FREGALAFTAAIFDSTNKNELVPDATNPSIFVQVGEREVKGIELGIVGKLTENWDLSA
                     GLAKMDTEVVQGSSAQTGSQINWSPELTFSSWTTYRTPFGLTIGGGARYVDTVARSIS
                     NNVLRTTNVPTAPEDWVVDAMLGYQVNEKVTIQLNGYNLLDEEYIATLNNGGSRYIPG
                     APLSALLSVNFTF"
     gene            complement(3160..4623)
                     /gene="zwf"
                     /locus_tag="VIV63_03620"
     CDS             complement(3160..4623)
                     /gene="zwf"
                     /locus_tag="VIV63_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY6123714.1"
                     /translation="MAKVPAGVGPRFPQVVVLFGATGDLARRKLIPGLLHLARAGFIP
                     GCRIIGVSLDPFDADSFRNFAREALNEFSARKVEGDDWSSFANSLDYVPLSAGPAVLA
                     EAVKRAEVSIGAESRRLHYLSVPPNAALSAVRMLGDAGLVQNSRIIMEKPFGTDLASA
                     VQLNAKLHEVFAEEQIFRIDHFLGKEPAQNILAFRFANGLFEPIWNRNFIDHVQIDVP
                     ETLGLGKRSAFYESTGAYRDMVVTHLFQILGFMAMEPPTHLAPKPISEEKNKVFRSML
                     PIQPTDVVRGQYIGYRGEEGVAPESDTETFIALKCSIDNWRWAGVPFYLRTGKRLAEG
                     QRIISIAFREPPKSMFPEGSGIGAHGPDHLTFDLADAAKTSLSFYGKRPGPGMRLDKH
                     SLQFAMNDTGQIGDVLEAYERLILDAMRGDHTLFTTAEGIERLWEVSVPLLESPPPVR
                     TYSQGSWGPNAIHQLIAPHAWRLPFERTWRDPNKMGS"
     gene            complement(4699..>5655)
                     /locus_tag="VIV63_03625"
     CDS             complement(4699..>5655)
                     /locus_tag="VIV63_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEY6123715.1"
                     /translation="IVSRWDDLYNQISAGVSIGYSGLPNWTFDIGGFATESRYSTQKP
                     TAADLEEWRELNLRWFQFGAFAPLFRSHGEAPLREIFNLAPQGSEVFNALLWYDKLRY
                     RLLPYTYTLAADTFHRDGTIMRGLAMDFANDAAARDVRDEFLFGKAFLVAPVYEHKAR
                     TRDVYLPAGAEWYDFHTGVKSAGGQNLLAEAPLSRMPLYVRAGSIVPVGPDVQYSSEK
                     PGAPITLFVFTGTDGAFDYYEDDGVSYGYERGEFARIPLRYDSAKGTLTIGARSGSYS
                     GMPEERTFKVRWIKEGSRAPADLDAAADATVEYKGAEVTVTL"
BASE COUNT          950 a         1735 c         1876 g         1094 t
ORIGIN      
        1 ccacggtttc gtccgattca ccgtgtttgc cgcgcagcgt ctggatggtc tgatcgagcc
       61 ggtacaacac ttcgcgatgc cgttcgtcgg ccaccatgct ctgcgtccag aagaacgaac
      121 acacgcgcgc accgcgcgtc accgggttca cgcgatgcag gctgccggag gtgtacagca
      181 ccaggtcgcc ggccggtagt ttgacgtcgt gctggccgaa ggtgtcgaac acggtgagct
      241 cgccgccgtc gtattcggac ggatcggtga ggaacagcgt ggaagagagg tcggtgcgga
      301 tgaaatgcga gctgccgggt acggcgcgca cggctccgtc aatgtgtgtg ccgtactgct
      361 cgccgccttc ataacgattg aacagcggcg gcacggtctt gagcggaagc gcggccgaga
      421 agaacagcgg actgcgcgac agcgcggtga ggatgagctc gcctaactgt cttccgaccg
      481 gcgacagttc gggcaactgg cggttcttct tgaccagcgc gccctgctcc ccgacggtgg
      541 ccttgccgtc ggaccattcg gcggaatcga gcaggcggcg gcattccgcc acctgctcgt
      601 gggtcaggac cttcgggaca tgaatcatca ttcggccgat gctcgcatga gcggctcagt
      661 gcgtgaaggt cagaacgtga agttcaccga cagcagcgcc gacaacgggg cgccggggat
      721 gtaacgcgag ccgccattgt tgagcgtggc gatgtattcc tcgtcgagca ggttgtagcc
      781 gttcagctgg atggtcacct tttcgttgac ctggtaaccc agcatggcgt ccaccaccca
      841 atcctcgggc gcggttggga cgttcgtcgt ccgcagcacg ttgttcgaga tcgagcgagc
      901 cacggtgtcg acgtaacgcg caccgccacc gatggtgagt ccgaagggcg tgcgataggt
      961 cgtccagctc gagaacgtga gttcgggcga ccagttgatc tgtgagccgg tctgcgcgga
     1021 gctgccttgc acgacttcgg tgtccatctt cgcgaggccc gcggacagat cccagttctc
     1081 ggtgagcttg ccgacgatcc cgagctcgat acccttcact tcgcgctcgc caacctgcac
     1141 gaagatgctg gggttggtcg cgtcgggcac cagttcgttc ttgttggtgc tgtcgaaaat
     1201 cgcggcggtg aaagccagcg ccccttcgcg gaattcccac ttggtgccga gctcgaggtt
     1261 ttcgccttcg gtcggctcga ggtccgtgcg gttcgcgttg ttggcgccgt tcggaggcgt
     1321 gagcgtgaag ttggcgccgc ccggcggctg cttcgagttg gcgtgcgata gatagatgct
     1381 tccgttcggc agcggcttgt acaacacgcc gaccttgtac gagaacaacg tatcgtccgt
     1441 gccgagttgc gcgggcgtga gcacctggtt gggtaccgtg ccggtgagcg ccatgctttc
     1501 gaagtcggtg tcgtacgtgt cgaagcggaa accacccgtc acttcccact tctcggcgaa
     1561 gctcgcggtg tcgaacaggt aggcgccgat ggtttgggtt tcaccgcgcg tgtacacgcc
     1621 gttgcgcacg ggatcgtaaa gcggcggcgc gtcggtgagg cccggctcgt acgggttcgc
     1681 cggctcgagc gtgccgaggc tggcggccgc ataggcgggg ttgtattgct cctcggagat
     1741 gaactcgacg cccgtcgtga tggcatggct cacgccgccg gccgcgacgt taatggtgag
     1801 gttggtctgg ttcgtcctga gcgtgttctc ctggaacttg ctctgacgcg tgcggttcac
     1861 cgtccacgta tcgggagtcg ggctcggaat ggtcacggcg ttgacgcccg tgagcacata
     1921 gaactgttcg agacggccgt agcgcgtgct gttgcgcagg gatacgttgt cgctgaagtc
     1981 gtgctcgaaa cgggcggtga acatcgtgcc cgaggtttcc tcgaagtcgc tgcgataacc
     2041 gtagtagttc tcgctgtcca cgggcgcggc gatcacgccg ggcgcgcgcg ggagcggcag
     2101 cggcggatcc ggattcgggt cgaggtcgcg gttgtagaag ccatcgactc cgagagtcgt
     2161 gacaccgcca tccggcacgt tgttctgctc ggtgtgcagt agatagaaat agctgcgcgt
     2221 atcgccctcg aggccaaacg cgatcgacgg cgccacggcc cagcccttgc gctcgatgaa
     2281 gtcgcggcca tccacttcgc cgtcctggcc catcacgttg aggcggagcg cggtgccgct
     2341 gccttcgaag cgatggttga tgtcgcccgt gatgcgcgca ttcgacgcgg tgccgtagct
     2401 ggccgtgccg ccggtgaagc tatccaccgt gggcaccttg ctggccatgt tcacgtagcc
     2461 cgaagcggcg ccacggccgt agtccggacc cgccggaccc ttggcgattt ccacctgctc
     2521 ggtgttgaag gtgtcgcgcg tgacggtgcc caggtcgcga atgccgtcga tgaagatgga
     2581 accctgcgtg tcgaatcccc gcatgaaaat ggaatcgccg gtggcggtgt tgccgttctc
     2641 gccgagcagg aacgtgacgc ccggggtatt gcgcagcgcc tgtgagagcg tcgtggcgcc
     2701 ctgttgcttg aggacttcct tgctcaccac cgcgatggtc tgcggcgtgt cgagcagcgg
     2761 ctgggtgaac ttcggcgact cgaccctatc gacctttggc gtctcgaccg gctccggcgc
     2821 ggcgaccgaa accttcggca tcgtcttcgc ttcttcggtt gcggtttgag ctttggctgc
     2881 ggctgcggac agcgcaagac tcacggccac ggcaatggac agattcgaca cggttggctc
     2941 ccagatgcgg ttgttctgat ctcccgcgcg ctctggctat cccggtcggg agtggctggc
     3001 tatggacggg gtgctgaaac ggcgatcact ccgtatcagc aacggcccgc atcgtattgc
     3061 gaatcattct tgtttgcaac cgcgaatgat tgctatctga gacacttatt aatggtgcca
     3121 ggcaccgatt tctagaaaac ggtgcctggc accgtttctt cagctcccca tcttgttcgg
     3181 gtcccgccac gtgcgttcga acggcaggcg ccaggcatgc ggcgcgatga gctggtggat
     3241 ggcgttcggg ccccacgacc cttgcgaata ggtgcgcacc ggcggcggcg attcgagcag
     3301 cggcaccgag acttcccaca gccgctcgat accttcggcg gtggtgaaca gcgtgtgatc
     3361 gccgcgcatg gcatcgagga tcagcctttc ataggcttcc agaacgtcgc cgatctggcc
     3421 ggtgtcgttc atggcgaact gcaggctgtg cttgtcgagg cgcatgccgg gcccgggtcg
     3481 cttgccgtag aacgacagcg aggttttcgc ggcgtcggcg aggtcgaacg tcaggtgatc
     3541 ggggccatgt gcgccgatac ccgagccttc ggggaacatg ctcttcggcg gctcgcgaaa
     3601 cgcgatggag atgatgcgtt ggccttccgc cagccgcttg ccggtgcgta gatagaacgg
     3661 cacgccggcc cagcgccagt tgtcgatcga acacttcaac gcaatgaagg tttcggtgtc
     3721 ggactcgggc gccacgccct cctcgccgcg atagccgatg tactgcccgc gcaccacgtc
     3781 cgtgggctgg atcggcagca tgctgcgaaa caccttgttc ttctcttcgc tgatgggctt
     3841 cggcgcgaga tgcgtgggcg gttccatggc catgaacccg aggatctgga acaggtgcgt
     3901 gaccaccatg tcgcgatacg cacccgtgga ttcgtagaac gccgagcgtt tgccgagacc
     3961 cagcgtctcg ggcacgtcga tctgcacatg gtcgatgaag ttgcggttcc agatgggctc
     4021 gaacaatccg ttcgcgaaac ggaacgcgag gatgttctgc gccggctcct tgccgaggaa
     4081 atggtcgatc cgaaagatct gctcctcggc gaacacctcg tgcagtttgg cgttgagctg
     4141 caccgcgctc gcgaggtcgg tgccgaaggg cttttccatg atgatgcgcg agttctgcac
     4201 caggcctgca tcgcccagca tgcgcaccgc ggacaatgcc gcgttgggcg gcacggatag
     4261 atagtgcagg cgccggctct ccgcgccgat ggagacctcc gcgcgcttca cggcttcggc
     4321 caggaccgcc ggaccggccg acagcggcac gtagtcgagt gagtttgcga agctgctcca
     4381 gtcgtcgcct tcaaccttgc gcgcggagaa ttcgttcagc gcttcgcgcg cgaaattgcg
     4441 gaagctgtcg gcatcgaacg ggtcgagcga gacgccgatg atgcggcagc cggggatgaa
     4501 tcccgcgcgc gccagatgca gcaggcccgg aatgagcttg cggcgggcaa ggtcacccgt
     4561 cgcgccgaac agaaccacga cttgcggaaa tcgcggacct acgcccgccg gaaccttcgc
     4621 cacggtgcac tcccattctt attagtagag aacacccctc gtgacgaagg ttttaccaca
     4681 gtggggacag gccccgcttc acagggtgac ggtcacttcc gcgcccttgt actcgaccgt
     4741 ggcatccgcc gcggcgtcca ggtcggcggg cgccctggag ccctccttga tccagcgcac
     4801 tttgaacgtg cgctcctcgg gcatcccgga ataactgccc gatcgcgcac cgatggtcag
     4861 cgttcccttg gcggagtcgt aacgcagtgg gatgcgcgcg aattccccgc gctcgtagcc
     4921 gtagctcaca ccgtcatcct cgtagtagtc gaaggcacca tcggtgcccg tgaagacgaa
     4981 cagcgtgatt ggtgcgcccg gcttctccga ggagtactgc acgtccggac cgaccggcac
     5041 gatggaaccc gcgcgtacat aaagcggcat gcggctgagc ggcgcttccg ccagcaggtt
     5101 ttgtccgccg gcagacttca cgccggtatg gaagtcgtac cactcggccc cggccggtag
     5161 atagacatca cgtgtgcgcg ccttgtgctc atacaccggc gccacgagga acgccttgcc
     5221 gaacaggaac tcgtcgcgca cgtcgcgcgc ggccgcgtca ttggcgaagt ccatggcaag
     5281 tcctcgcatg atggtgccgt cccgatggaa cgtgtccgcc gccagcgtgt acgtgtaggg
     5341 caacagccgg taccgcagct tgtcgtacca cagcagggcg ttgaacacct ccgagccctg
     5401 cggagcgaga ttgaagatct cgcgaagggg ggcttcacca tgcgaacgga acagcggcgc
     5461 gaacgcgccg aactggaacc agcgcaggtt gagttcgcgc cactcctcga gatcggccgc
     5521 ggtcggcttc tgcgtgctgt agcggctctc ggtggcgaag ccgccgatgt cgaacgtcca
     5581 gttgggcagg cccgaataac cgatgctcac gcccgcggag atctggttgt acagatcgtc
     5641 ccagcgcgaa acgat
//
LOCUS       DATDEU010000144         3280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1192215, whole genome shotgun sequence.
ACCESSION   DATDEU010000144 DATDEU010000000
VERSION     DATDEU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192215"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="VIV63_03630"
     CDS             <1..1861
                     /locus_tag="VIV63_03630"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123716.1"
                     /translation="SIRKVSLEIQTDARFTTVGNSATQIEFTRPEEKLGFLTVRSGTT
                     LGKGKIRVIATSGKFRAEHEVWLEVRTPNAPSSRFLRATLAPGESWKGDLTAFGLPGT
                     QVANLEVSALPPINFDGRLEYLIHYPYGCLEQTTSSVFPQLYLPALIKLDQNRRLEVE
                     NNIRAGLARLRSLQHPSGGFAYWPGIWNTDGGRDWRNNWGTTYAGHFFLEAEKAGYTL
                     PGDMKSSWLRYQKDAAQQWNPRRDEHGKYAPDEVHAERYAQGYRLYTLALAGGAEIGA
                     MNRLRENASLTLPERWLLASAYKLAGKADVATALIEKDRLQALVFADANPYTFGSLLR
                     DRAVVLMGMTLLGREAETAALLDDVADQLSDGSWYSTQSVAFALVAVAHNTGTKPFTG
                     FSFDYSATAKKQSVKAESPVANIKLPAPPATGAPVTLTNTSDRKLYATVGIRAVPRSG
                     EEDISSNGLDLRVEYSDADGKVLDVRRIAQGSDLIAQLTVKNVSRRQLDNLALQQLVP
                     AGWEIRNDRLEGVDTQGERSTEPQPLGQFWWVPSEWRNQSMRTAEYVDIRDDRVQRFF
                     SLKPGESIFFETRLNAAYLGKFYLPGASVEAMYEATQHARVKGQWVEVVSAQR"
     gene            1831..>3280
                     /gene="pbpC"
                     /locus_tag="VIV63_03635"
     CDS             1831..>3280
                     /gene="pbpC"
                     /locus_tag="VIV63_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEY6123717.1"
                     /translation="MGRSRLRTALTRIVATRRRRVIAAVLAATCAAAAWFWAWLPDEL
                     FVSPESYVLEARDGTLLSARIASDGQWRFPPQATVPRKFRRALLVFEDKRFEQHPGVD
                     ALAVARAMRLNVSAGRVVSGGSTITMQLARLSRGGEPRNLANKLTEAALALRIEASYG
                     KDEILALYAGHAPFGGNVVGLEAAAWRYFGRDPETLSWAEAATLAVLPNNPALVHLAR
                     NRERLRSKRDFLLRRLHDAGDLTALDLDLALSEPLTAEPHDLPDLAPHLLDTLRAQDP
                     ARHRIVTTLDARLQRDATQLTREHSTALARQGVHNAAALIVDNTTFEVLAYVGNSSEA
                     LDASRGHAVDIIRRPRSTGSILKPMLYAAMLEDGSLTPRMLLPDVPTHYEGFAPENFD
                     RQYRGAVPADEALAHSLNVPAVRMLKTYGVARFADLLRAAGMTTLTRPGDDYGLTLIL
                     GGAEGNLGDVSGMYASLAGLARAGAADAPPAFR"
BASE COUNT          573 a         1142 c         1065 g          500 t
ORIGIN      
        1 gtcgatccgg aaagtctcgc tcgagattca gacggacgcg cgtttcacca ccgtcggcaa
       61 ttcggcgacg cagatcgagt tcacgcgtcc ggaggaaaag ctcggcttcc tcaccgtgcg
      121 gtcgggcacg acgctgggca agggcaagat ccgcgtgatc gcgaccagcg gcaagttccg
      181 tgcggaacac gaggtgtggc tcgaggtgcg cacaccgaat gcgccctcct cgcgcttcct
      241 gcgcgccacg ctcgcgcccg gagaatcgtg gaagggagat ctcacggctt tcggtctgcc
      301 cggaacccag gtcgcgaacc tcgaggtgtc agcgctgccg cccatcaact tcgatggcag
      361 gctcgaatac ctcatccact acccttatgg gtgcctggag cagaccacct cgagcgtgtt
      421 cccgcagctc tatctaccgg cgctgatcaa gctcgaccag aaccggcgcc tggaagtgga
      481 gaacaacatc cgcgcgggcc tcgcgcgcct gcgaagcctg caacacccca gcggcggctt
      541 cgcctactgg ccgggaatct ggaatacgga tggcggccgc gactggcgca acaactgggg
      601 caccacgtat gccgggcact tcttcctcga agccgaaaag gccggataca ccttgccagg
      661 cgacatgaag tcgtcgtggc tgcgttatca gaaggacgcc gcgcagcagt ggaatccgcg
      721 ccgcgatgag cacggaaaat acgcccccga cgaagtgcat gccgaacgct acgctcaagg
      781 ctaccggctc tatacgctgg cgttggccgg cggtgcggag atcggcgcca tgaaccgcct
      841 gcgtgagaac gcgtcgctca cactgccgga acgctggttg ctcgcctcgg catacaagct
      901 ggcgggcaag gccgacgtcg cgactgcgct gatcgagaag gaccggctgc aggccctggt
      961 gttcgcggac gccaatccct acacgttcgg atcgctgctg cgcgatcgcg ccgtggtgct
     1021 catgggcatg acgctgctcg gccgcgaggc tgaaaccgcg gcgctgctcg acgacgtggc
     1081 cgaccaactc tcggacggga gctggtacag cacgcagtcg gtggccttcg ccctggtggc
     1141 ggtcgcgcac aacacgggca cgaaaccgtt caccggcttc agcttcgact actcggccac
     1201 ggcgaagaag cagtcggtga aggcggagtc cccggtcgcg aacatcaaac tacctgcccc
     1261 gccggccacg ggtgcgccgg tcacgctcac gaacacgtcg gatcgcaagc tttacgccac
     1321 ggtcggcatt cgcgccgttc cgcgcagtgg cgaggaagac atctcatcca atggtctcga
     1381 tctccgggtc gaatacagcg acgccgacgg caaggtcctc gacgtgcgcc gcatcgcgca
     1441 gggcagcgac ctcatcgccc agctcacggt gaaaaacgtc agccggcgcc agctcgacaa
     1501 tctggcactc cagcagctcg ttccggcggg ctgggaaatc cgcaacgacc gactcgaggg
     1561 cgtcgacacc cagggcgaga ggagcacgga accgcagccg ctcggccagt tctggtgggt
     1621 accgtccgaa tggcgtaacc agtcgatgcg taccgcggaa tacgtggaca tccgcgacga
     1681 ccgtgtgcag cgattcttca gcctcaaacc gggcgagtcg atcttcttcg aaacgcggct
     1741 caacgccgcc tacctcggga agttctatct accgggcgcc agcgtcgaag ccatgtacga
     1801 ggccacccag cacgcgaggg tcaagggaca atgggtcgaa gtcgtctccg cacagcgctg
     1861 acacgcatcg tcgccacccg gcgccgccgc gtgatcgcgg cggtgctggc ggcgacctgc
     1921 gcggcggcgg cctggttctg ggcgtggtta cccgacgaac tgttcgtgtc gcccgagtcg
     1981 tacgtgctcg aagcgcgcga cggcacgttg ctgagcgcgc gcatcgccag cgacggccaa
     2041 tggcgttttc cgccgcaggc cacggtaccg cgcaaattcc gccgcgcctt gctggtgttc
     2101 gaagacaagc gcttcgaaca gcatccgggt gtcgatgcgc tcgcggtcgc gcgcgccatg
     2161 cggctcaacg tgagcgcggg ccgcgtggtc agtggcggca gcacgatcac catgcagctg
     2221 gcgcggctgt cgcgcggcgg tgagccccga aacctggcga acaagctgac cgaagcggcc
     2281 ctcgcactgc gcatcgaggc ttcgtacggc aaggacgaaa tcctggcgct gtacgccgga
     2341 catgcgccgt tcggcggcaa tgtcgtcggt ctcgaggcgg cggcgtggcg ctacttcggc
     2401 cgcgacccgg agacgctgtc gtgggccgag gccgcgacgc tcgcggtcct gcctaacaac
     2461 ccggcactgg tgcacctggc gcgcaatcgc gaacgccttc gcagcaagcg tgacttcctg
     2521 ctgcgccgcc tgcatgacgc cggcgacctc acggcgctcg acctcgatct cgcgctcagc
     2581 gagccgctga cggccgagcc gcacgacctg ccggatctcg cgccacatct gctcgacacc
     2641 ctgcgcgccc aggatccggc ccggcatcgc atcgtcacca cgctcgacgc acggttgcag
     2701 cgtgacgcca cgcaactcac gcgcgaacac tcgaccgcgc tcgcgcgcca gggcgtgcac
     2761 aacgccgcgg cgctgatcgt cgacaacacc acgttcgaag tgctcgccta tgtcggcaac
     2821 tccagcgagg cgctggacgc gtcgcgcggg cacgccgtgg acatcatccg tcgtccgcgc
     2881 agcacgggca gcattctcaa gccgatgttg tatgccgcca tgctcgagga cggctcgctg
     2941 acgccgcgca tgctgctgcc cgacgtaccg actcactacg aaggtttcgc gcccgagaat
     3001 ttcgatcgcc agtaccgcgg cgcggtaccc gccgacgaag cgctcgcgca ttcgctcaat
     3061 gtgccggcgg tccggatgct caagacctat ggcgtggcgc ggttcgccga cctgctgcgc
     3121 gcggccggca tgaccacgct cacgcgtccg ggcgatgact acggcctcac gctcatcctc
     3181 ggtggcgcgg aaggcaatct cggggacgtg tccggcatgt acgcgagcct cgcgggcctc
     3241 gcccgcgccg gagcggccga cgcgcccccg gcgttccgcg
//
LOCUS       DATDEU010000145         6410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_900377, whole genome shotgun sequence.
ACCESSION   DATDEU010000145 DATDEU010000000
VERSION     DATDEU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900377"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1693)
                     /locus_tag="VIV63_03640"
     CDS             complement(248..1693)
                     /locus_tag="VIV63_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123718.1"
                     /translation="MKFPEEFVIRILLVLAALISAGCVTRGGVAEEAEPTVIDAVPES
                     TVETATPVAEESKEQEGEVPLAVADSEQTDESLAEVVVQGRVRTRHEAIHDLVAEGQA
                     LLSDVDLKFVLTGKGKRQTLRGRPVAFALWSEAKQQWTVAQVELPRPPIKWKPGRKPL
                     SFRMLTPGIQARHVKGTGAERLMFAFTADGEPLKVYGRKFPVFDNKLVKRRQWASVVQ
                     SAKSIVYLPFTEDTFDPGFVSGGREFLQATVGKALEELRLAGVPSAAFPGELLADAIP
                     PQVVTTLAVIEQTDDRDYRQKQGVAFEEVLSQYGLKQEEAYRYSVSSAKALGPMQFTN
                     RRGNGTYALVVRRCRGAKLDPVFESGATNLLNAMKAAICLLDLELAGMRSDIREAYRD
                     NPVVLGIFPVAAYNGGPRNVTKLYNVLSRMKVGLDELTRPPAELPDSPVPCPCVWKTD
                     GDTARPVTIPKYNNENRWYIEKYQSIVSLFE"
     gene            complement(1690..2517)
                     /locus_tag="VIV63_03645"
     CDS             complement(1690..2517)
                     /locus_tag="VIV63_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6123719.1"
                     /translation="MKLWPLLLMALLPAALLAADSPPKPGTAFITGSDRGIGFALVQE
                     FESRGWQVIATTRDPAKATELNAFAKAHPRVTVEKLDVTDTAGIDALAVKLKDRPIDA
                     LVNNAGIAGTFEGQQIGTLDQDEFVRVMRVNSYGPLRISAAFLDHVAASKQRKIIGIS
                     SGYGSLASVESLASAGYPSSYFYAMSKAALNMGLREFALDPGAKRGVTTVLFSPGAVD
                     TDMQRTIRADVAQAGKPITLPTRTPAEVAHAMVLAIEQLTPEQNGRFYSVTGKEVPW"
     gene            2603..3058
                     /locus_tag="VIV63_03650"
     CDS             2603..3058
                     /locus_tag="VIV63_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6123720.1"
                     /translation="MKNKKFPAWMLAIAFCLMGSAQASPAPAHEEAVAQTLRLMYVAL
                     TQEDAAQLRAVTTADFYAFDGGEKMTGDELMALIKSAHAAGKNFVWTVTEPRVRIENA
                     VAWITYLNRGSVQDAAGKKDVSWLESAVLLKEGGTWRIQFFHSTRVPAK"
     gene            complement(3060..4643)
                     /gene="guaA"
                     /locus_tag="VIV63_03655"
     CDS             complement(3060..4643)
                     /gene="guaA"
                     /locus_tag="VIV63_03655"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY6123721.1"
                     /translation="MNPQTDIHSSRVLILDFGAQYTQLIARRVREQGVYCEILPWDVT
                     NDDVRKWKPKGIILSGGPESVTDKEPPRAPQSVFELGVPVLGICYGMQTMAQQLGGRV
                     VNSNHREFGYAEVTSIAPNKLLDGLFDRQDAEGRAVLDVWMSHGDKVDTLPAGFTAVA
                     RTANAPLAAMADESRKFYAVQFHPEVTHTLQGKQLLERFVREICGCQPLWSVGNIIED
                     AIARVRAQVGKGKVLLGLSGGVDSSVVAALLHKAIGDQLTCVFVDHGLMRLNEGDQVM
                     KIFADNLGVKVIRVDAEQRFLDALRGETDPEKKRKIIGGLFVKVFDEEAQKLTEVDWL
                     AQGTIYPDVIESAGSKTGKAHVIKSHHNVGGLPANMRMKLVEPLRELFKDEVRRLGVE
                     LGLPREMVYRHPFPGPGLGVRILGEVRKDYADLLRQADAIFIEELRRHDLYDKTSQAF
                     AVFLPVRSVGVMGDGRRYDYVVSLRAVETVDFMTAHWARLPYDFLDICSRRIVNEVKG
                     ISRVVYDISGKPPATIEWE"
     gene            complement(4667..6154)
                     /gene="guaB"
                     /locus_tag="VIV63_03660"
     CDS             complement(4667..6154)
                     /gene="guaB"
                     /locus_tag="VIV63_03660"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY6123722.1"
                     /translation="MPTQPPSPLRILEEALTFDDVLLVPAYSEILPREVDLGTRLTRD
                     IRLNLPLVSAAMDTVTEARLAITMAQEGGIGIVHKSMTIAGQAGEVARVKKFESGVIR
                     DPITVAPDMTIRQVIDLTRSKGISGVPVVQGSKVVGIVTHRDLRFEVKLDAPVSSVMT
                     PKERLVTVRENAPKDEVLLLLHKHRIEKVLVVNSDQELRGMITVKDFQKATEFPRACK
                     DPSGRLRVGAAVGTAQDTMERVAALREAGVDVVVVDTAHGHSRGVLDMIARIKSKWGD
                     LQVIGGNVATAEAARDLVKAGVDGIKVGIGPGSICTTRIVAGVGVPQISAVSNVAAAV
                     DNQVPVIADGGIRFSGDIAKAIVAGADCVMIGGLFAGTEESPGDVELYQGASYKAYRG
                     MGSLGAMAERHGSADRYFQDTAAELEKLVPEGVEGRVPYKGSLVSILHQLAGGLRAAM
                     GYTGSTSIAVMRTNPRFVRVTGAGMRESHVHDVQITKEAPNYRVS"
BASE COUNT         1111 a         2034 c         2036 g         1229 t
ORIGIN      
        1 ccctcgcctt tcacgtcttc gatcgacgcc agggaacgtt cgacgagttg ccggctcgtg
       61 agcttgccgg ctgccatctg gcgcgagagt tcggggagat tcatgacggc agggtcgcta
      121 accgatcgcg aacgggcaag acttttcgaa gtgccgagcg ccagggcggc gatcgaggcc
      181 gcgccggcgc gaacggcacc gcgcaggaat tggcgacgac ttcggaacat gtccccgaga
      241 atacccgcta ctcgaacagg ctgacgatgc tctggtactt ctcgatgtac caccggttct
      301 cgttgttgta tttcggaatc gtcaccggcc gtgcggtgtc gccgtccgtc ttccacacac
      361 agggacatgg caccggcgag tccgggagtt ccgctggcgg gcgagtcagc tcgtcgagcc
      421 cgactttcat ccgggacaac acgttgtaga gcttggtcac gttccggggc ccgccgttgt
      481 aagcggcaac gggaaagatt ccgagcacga ccgggttgtc gcgatacgcc tcgcgaatgt
      541 ccgaccgcat cccggccagt tccagatcca gcaggcagat cgcggccttc atggcgttca
      601 gcagattcgt cgcgccactt tcaaaaaccg ggtcgagctt tgcgcccctg cagcgccgca
      661 ctaccaatgc gtaggtcccg ttgccccggc ggttcgtgaa ctgcatggga cccagcgctt
      721 ttgcgctcga gacgctgtac cggtacgcct cctcctgctt gagaccgtac tggctcaaca
      781 cctcttcgaa ggcgacccct tgcttctgac ggtagtcccg atcgtccgtc tgctcgatga
      841 ccgccagtgt agtgactact tgcggtggaa tggcatcggc gagcagttca cccggaaagg
      901 ccgcgctcgg cacgccggcg aggcgcaact cttcgagcgc cttgccgacg gtggcctgca
      961 ggaattccct tccaccgctg acgaaacccg ggtcgaaggt gtcttcggtg aagggtagat
     1021 agacgatcga cttcgcggac tggacgaccg atgcccactg cctgcgcttg accagcttgt
     1081 tgtcgaagac cgggaacttg cgtccgtaga ctttcaaagg ctccccgtcc gcggtgaaag
     1141 cgaacatgag ccgctcggcg ccggtgcctt tcacgtgccg cgcctggata ccgggcgtga
     1201 gcatgcgaaa ggaaagcggc ttcctgccgg gcttccactt gatcggcgga cgggggagtt
     1261 ccacctgggc gacggtccat tgctgtttcg cctcgctcca cagcgcaaag gcgaccggcc
     1321 gcccgcgcag cgtctgacgc ttgcccttgc ccgtcagaac gaatttgaga tcgacgtcgc
     1381 tgagcagtgc ctgcccttcg gcgacgagat cgtggatggc ctcgtgacga gtgcggacgc
     1441 ggccctggac gacgacttcg gccaggctct cgtcggtctg ctcggaatcg gccacggcaa
     1501 gcggaacctc gccttcctgc tctttcgatt cttcagcgac cggagttgcg gtctcgaccg
     1561 tggattccgg cactgcgtca atcactgtcg gctcggcttc ctcggcgacg ccgccgcggg
     1621 tcacacatcc tgccgaaatc agcgctgcaa gtaccagcag gattcgaatc acgaactcct
     1681 ccggaaattt caccagggca cttccttccc cgtcaccgaa taaaacctgc cgttctgttc
     1741 cggcgtcagc tgctcgatgg ccaacaccat ggcatgtgca acttccgcgg gcgtgcgagt
     1801 cggcaatgtg atcggcttgc ctgcctgcgc gacgtccgca cgaatcgtgc gctgcatgtc
     1861 ggtatccacg gcgcccggcg agaacagcac cgtggtcacg ccgcgcttcg cccccgggtc
     1921 cagcgcgaac tcacgcaggc ccatgttcag cgcagccttg ctcatggcat agaaatacga
     1981 gctgggatat ccggcactcg ccagggattc caccgaggcg agcgaaccgt aaccactgct
     2041 gatgccgatg atcttacgct gcttgctggc cgccacgtga tcgaggaacg cagccgaaat
     2101 ccgcagcggt ccgtaactgt tcacgcgcat cacgcggacg aattcgtcct gatcgagcgt
     2161 gccgatctgc tgaccttcga aggtgccggc gatgccggcg ttgttgacca gagcatcgat
     2221 gggacgatcc ttcagcttga cggccagcgc atcgatgccc gccgtgtcgg tgacatcgag
     2281 cttctcgacc gtgactctcg gatgggcttt cgcaaatgcg ttgagttcgg tggccttcgc
     2341 gggatcccgc gtcgtggcga tgacttgcca accgcggctc tcgaactcct gcaccagcgc
     2401 gaaaccgata ccgcggtcgg acccggtgat gaatgcggtg ccgggcttgg gcggcgaatc
     2461 agcggcgagc aatgccgcgg gcaacagcgc catgagcaac aaaggccaga gtttcatcgg
     2521 cgaatttccg gtaacagcgg gtgtgaattc agtatgcctg atgccacttg agcgcgagcc
     2581 ctgcgctggt aaggtggccg ccatgaaaaa caaaaagttt ccagcctgga tgctcgccat
     2641 cgccttctgc ctgatgggtt cggcacaggc ctctccggca cccgcgcatg aagaagccgt
     2701 ggcgcagacg cttcgcctga tgtacgtcgc cttgacccaa gaggacgccg cccagctccg
     2761 agccgtaacc actgccgact tctatgcgtt cgatgggggc gagaagatga ccggcgacga
     2821 actcatggcg cttatcaaga gcgcgcatgc agccggcaag aatttcgtgt ggactgtcac
     2881 ggagcccagg gtgcgtatcg aaaacgccgt ggcgtggatc acctacctca atcgcggctc
     2941 cgtgcaggat gccgcgggga agaaggacgt gagctggctg gagtcggcgg tgctgctgaa
     3001 ggaaggcggc acctggcgca tccagttttt ccacagcacc cgggtgccgg cgaagtgacc
     3061 tactcccact cgatcgtggc ggggggcttt ccggagatgt cgtagacgac gcgcgagatg
     3121 cctttcactt cgttcacgat tcggcgtgaa catatatcga ggaagtcgta cggcagtcgc
     3181 gcccagtgcg cggtcatgaa atccacggtc tcgacggcgc gcagactgac tacgtaatcg
     3241 taacggcggc cgtctcccat gacacccacc gaacgcacgg gcaggaacac ggcgaaagcc
     3301 tgcgaggtct tgtcgtacag atcgtggcgc cgcagttctt cgatgaagat ggcgtcggcc
     3361 tggcgcagca ggtcggcgta gtccttgcgc acttcgccga ggattcgcac accgagcccc
     3421 ggacccggga acggatggcg gtacaccatt tcgcgcggca aaccgagttc cacgcctaac
     3481 cttcgcactt cgtccttgaa caactcgcgc agcggctcga cgagtttcat gcgcatgttg
     3541 gccggcaggc cgcctacgtt atggtgcgac ttgatcacat gagccttgcc ggtcttgctg
     3601 cccgcggact cgatgacgtc ggggtagata gtgccctgcg ccagccagtc cacttccgtc
     3661 agcttctgcg cctcttcatc gaacaccttc acgaagaggc cgccgatgat cttgcgcttc
     3721 ttttccgggt cggtctcgcc gcgcagcgcg tcgagaaagc gctgttcggc atccacgcgg
     3781 atgactttca cgccgaggtt gtcggcaaag atcttcatga cctggtcgcc ttcgttgagg
     3841 cgcatgaggc cgtgatcgac gaacacgcag gtcagctggt cgccgatcgc cttgtgcagc
     3901 agcgcggcga cgaccgagga gtcgacgccg ccggacaggc ccagcaacac cttgcccttg
     3961 cccacctgcg cacgcacgcg cgcgatggcg tcttcgatga tgttgccgac gctccacaaa
     4021 ggctgacatc cgcagatctc gcgcacgaag cgctcgagca gttgtttgcc ctgcagcgtg
     4081 tgagtgactt ccgggtggaa ttgcacggcg tagaacttgc gcgattcgtc ggccatggcc
     4141 gcgagcggcg cgttggcggt gcgcgcgaca gccgtgaaac cggcgggcag cgtgtcgacc
     4201 ttgtcgccgt ggctcatcca cacatcgagc acggcacggc cttccgcgtc ctggcggtcg
     4261 aacaggccgt cgagcagttt gttgggcgcg atgctggtga cttcggcgta gccgaactcg
     4321 cggtgattcg agttcacgac gcggccaccc agctgctgcg ccatggtctg cattccgtag
     4381 cagatgccga gcaccggcac gccgagctcg aataccgact gcggcgcgcg cggcggctcc
     4441 ttgtcggtga cggattcagg gccgcccgaa aggatgatgc ccttcggctt ccacttgcgg
     4501 acgtcgtcgt tggtgacgtc ccagggcagg atttcgcagt agacgccctg ttcccgcacg
     4561 cggcgcgcga tgagctgggt gtactgcgcg ccgaagtcca ggatgagtac gcgcgaggag
     4621 tggatgtcgg tttgtggatt catcggactc gccgcaacgc tgttcatcag gaaactcggt
     4681 agttaggtgc ttctttggtg atctgcacgt cgtgcacgtg gctctcgcgc atgccggcgc
     4741 cggtcacccg cacgaacctc gggttggtgc gcatgaccgc gatgctggtg ctgcccgtgt
     4801 agcccatggc ggcacgcagg ccaccggcga gctggtgcag gatggacacg aggctgcctt
     4861 tgtagggcac acgtccttcg acgccctcgg gaacgagctt ctcgagttcc gcggccgtgt
     4921 cctggaaata tcgatcggcc gagccgtggc gctctgccat ggcgccgagc gaacccattc
     4981 cgcgataggc cttgtacgat gcgccctggt agagctcgac gtctccggga gattcttcgg
     5041 taccggcgaa caggccgccg atcatcacgc agtccgcgcc cgcgacaatc gccttggcga
     5101 tgtcgcccga gaagcggatg ccaccgtcgg ctatgaccgg aacctggttg tccacggcgg
     5161 cggcgacgtt cgagacggcg gagatctgcg gaacaccgac acccgccacg atacgcgtgg
     5221 tgcagatgga gccgggtccg atgccgacct tgatgccgtc cacgccggcc ttcacgagat
     5281 cacgcgccgc ttcggcggtc gcgacgttgc cgccgatgac ctgcaggtcg ccccacttgg
     5341 acttgatgcg cgcgatcatg tcgagcacgc ccctggagtg accatgcgcg gtgtcgacca
     5401 cgaccacgtc gactccggcc tcacgcagcg ccgcgacgcg ctccatcgtg tcttgtgcgg
     5461 tgccgaccgc ggcgccgacg cgcaggcgac ccgaagggtc tttgcaggcg cgcgggaatt
     5521 ccgtggcctt ctggaaatct ttcacggtga tcatgccgcg cagttcctgg tcgctgttca
     5581 cgaccagcac tttttcaatg cgatgtttgt gcaacagcaa aagcacttcg tccttcggcg
     5641 cgttctcgcg cacggtgacg agccgctcct tcggggtcat gaccgaagac acgggcgcgt
     5701 ccagcttcac ttcgaagcgc aggtcgcggt gcgtgacgat gccgacgacc ttggagccct
     5761 gcaccaccgg tacgccggag atgcctttcg accgggtgag gtcgatgacc tggcggatgg
     5821 tcatgtccgg cgccacggtg atcggatcgc ggatcacacc gctttcgaat ttcttgacgc
     5881 gcgcgacttc accggcctgg ccggcgatgg tcatgctctt gtggacgatg ccgatgccgc
     5941 cttcctgcgc catggtgatg gcgagacgcg cttcggtgac ggtgtccatg gcagcggaca
     6001 cgagcggaag attgagccgg atgtcgcgcg tgaggcgcgt acccaggtcg acttcgcgag
     6061 gcaggatttc cgagtaggcc ggcaccagaa gaacgtcgtc gaaggtcaac gcttcttcga
     6121 gaattcgtaa cggagagggg ggttgggtgg gcatgcggga ttgtacgcaa gtgactaccg
     6181 cgggtaaacg ggtggggact ggctccgttt ttgtcttcaa aaacggtgcc tgtccccacg
     6241 caatcctgcg tgtgacgtag cgcacacccc gcgcgtcgct tgcccgcgac gctggcgccc
     6301 attcccaagc tgttagatca tggcgcggcc ctgtatccag ggctgtttgc ggagcttcga
     6361 atgcgccttg ggctgatcct gcttctgtgt cttctgaaca ccggctgcgc
//
LOCUS       DATDEU010000146         3504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_755101, whole genome shotgun sequence.
ACCESSION   DATDEU010000146 DATDEU010000000
VERSION     DATDEU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755101"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..762)
                     /locus_tag="VIV63_03665"
     CDS             complement(148..762)
                     /locus_tag="VIV63_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEY6123723.1"
                     /translation="MPDQAEQQEPGEVTRLLTRWTEGDASAIEALWPLVYDDLRRLAR
                     RQLAGERGDHTLQRTALVNEAFIRLSGQRAIDWLNREQFLSLAAKIMRRVLVDYARRR
                     GAQRRGDGAPHISMHDTQAALEVDQVQAIPVFEDNGVDVVAIDAALLRLEKIDAPQSH
                     IVELRYFGGLTLEETAGVVGISLASVKREWAMARAWLRRELAAG"
     gene            907..>3504
                     /locus_tag="VIV63_03670"
     CDS             907..>3504
                     /locus_tag="VIV63_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123724.1"
                     /translation="MAVRIGSVLCVFSLLVALAGCGGGDGASPPPPASNIPPPPPPPP
                     PPPPVVGAAGGTVTGAGASVVVPAGAFTAGTTVRISQDSTGAPAIPADLSPSGSMYMI
                     TPHGGEFAEGVEVRLPVPNVTLTPFQEFKIAKAQPGEPWIVLEDTALNEGALSAKVDS
                     FSFFMVVVITYPLQIVEFPPMSMSTSISCTDNQCSGLIGSTTATFTVTTNNGQTPGNC
                     TAPIYGVGTGAGSSISWPQTGSSIGVAPMAATGGSYTRALNPGPASQFKFGVGRKCGV
                     NGRYSDFGYSNYKYVSWAFSYGPTLRIVRMPEQVDVVEGLVANVDVVMSGGAAQPADP
                     QSSSNTQYSVPTRTNRAIIDWERSDDGGASWRVVARSYQDEANSLPAGVGYPWRYWGI
                     RHGFVATAADQGALLRLHACYASPTPTEALPCIDSAISRINVLQQSALPTIVESPRSM
                     LVRTGQTASLSATASGLPAPTLQWQSRPANSTGAWSNVTTGTGATAANYTTAALVPGD
                     NGTQYRIVATNAVGVAESSAVTVSVSDLDVAPTIATQPASLSVASGSDAIFAVDARGT
                     EALSYQWFRNGVAIAGANSPVLRLPAVTDANAGSYSIGVSNSAGNATSNAAVLTVSAS
                     APAAVAPSIVTQPVSVTANVGSTATLAVGVDGTGPFTFQWRRDGVNVTGATSAVLTFP
                     AVALPNAGSFSVLVTNSAGSVTSGNAVLDVRGDSTATIPTITSQPSTLIVPFRGSGVL
                     AVGATGSGPLSYQWSKDGAELPGATLPVLDFAIVADVDVGRYTVTVSNNVGSVVSSAA
                     DIILLGAPVITQQPTDVTALEGGNAMFFVAASSSGLRYQWSANGNPIPGATAATLNLN
                     SVVLANNASV"
BASE COUNT          609 a         1208 c         1074 g          613 t
ORIGIN      
        1 acgccacttc ggcgatcagc tccgaatctc ctctcgcctc ccgcttgagc aaatcggggc
       61 gttcagactc tggtacctcg agcgcagcgg cgaacaacgc cttcacctgc agccagcgtt
      121 gcggcgagat ctcggtgtcc aaacgattca gcctgccgcg agttcgcgac gcagccaggc
      181 acgcgccatg gcccattcgc gtttcacgga cgccagcgag atgccaacca caccggccgt
      241 ttcttcgagc gtgagcccgc cgaaatagcg cagctccacg atgtgactct gcggcgcatc
      301 tatcttctcg agccgcaaca gcgccgcgtc gatcgccacg acgtccacgc cgttgtcctc
      361 gaagaccgga atcgcctgaa cctggtcgac ttcgagcgcg gcctgggtgt cgtgcatgct
      421 gatatgaggc gcgccatcgc cgcggcgttg tgcgccacgc cggcgtgcat agtcgaccag
      481 cacgcggcgc atgatcttcg cggccagcga caggaattgc tcgcgattga gccagtcgat
      541 cgcgcgctgg cctgacaacc ggatgaaggc ctcgttgacc agcgcggtac gttgcagggt
      601 gtgatccccg cgttcgcccg caagctggcg gcgcgcgaga cgacgcagat cgtcatacac
      661 cagcggccac aacgcctcga tcgcggaagc atcaccttcc gtccagcgcg tgaggagtcg
      721 cgtgacttcg cccggttcct gttgttctgc ttgatccggc acgcaatcga ttgtggccag
      781 aaaacgcgtg gcgtcaatga gatacgcgaa ttcctgcgtt aagtaaggcg gaaagttcag
      841 gaactgtgag ccgttttgcc ttggcttgcc gcgttgatcc cacgggtaac tatcaaggtg
      901 ggcgtcatgg ctgtgcgtat cggttccgtt ttgtgcgtgt tcagcttgtt agtggcgctc
      961 gccgggtgtg gcggcgggga tggcgcatcg ccaccgccgc ctgcgtcaaa catcccgcca
     1021 ccaccgccac cgcccccacc accgccgccg gtcgtcggcg cggccggtgg aaccgtgacc
     1081 ggagccggcg cgtcggtcgt ggttccagca ggcgcattca cggccggtac caccgtacgc
     1141 atttcccagg actcgacggg cgcgccagcg attccagccg acctgtcccc aagcggcagc
     1201 atgtacatga tcacgccgca cggtggcgag ttcgccgagg gcgtcgaagt gcgtcttccg
     1261 gttccgaacg tgacgctgac gccatttcag gaattcaaga tcgccaaggc gcagcccggc
     1321 gagccctgga tcgtgctcga agacaccgca ctgaacgaag gcgcgctgag cgccaaggtg
     1381 gacagcttct cgttcttcat ggtcgtggtg atcacctatc cactgcagat cgtggagttc
     1441 ccgccgatga gcatgagcac gtcgatctcc tgcaccgaca accaatgcag cgggcttatt
     1501 ggctcgacga cggccacttt caccgtgacc accaacaacg gccagacacc cggcaactgc
     1561 accgctccca tctacggggt cgggacgggc gcgggcagca gtatttcctg gccccaaacc
     1621 ggatcttcga tcggcgttgc gcccatggcg gcaactggcg gctcctacac ccgtgcactg
     1681 aaccccggtc cggcatcgca gttcaagttc ggcgtcggcc gcaagtgcgg agtgaacggt
     1741 cgctactctg actttggcta ttccaactac aagtacgtgt cgtgggcatt ctcgtacggc
     1801 ccgaccctgc ggatcgtgcg gatgccggaa caggtggacg tggtcgaagg actcgtcgcg
     1861 aacgtcgacg tcgtgatgtc aggcggcgca gcccagcccg ccgatccgca atcgtcgtcc
     1921 aacacccaat acagcgttcc cacccgcacc aaccgcgcca tcatcgattg ggaacgcagt
     1981 gacgatggcg gcgcttcctg gcgagtggtc gcccggtcct accaggatga ggccaacagc
     2041 ctgcccgccg gcgtcgggta tccgtggcgc tactggggca ttcgccacgg tttcgtggcc
     2101 acggccgccg accagggtgc gctgcttcga ctccatgcct gttacgcatc gccaactccg
     2161 accgaggcac tcccgtgcat cgacagcgcg atatcgcgga tcaatgtcct gcaacagagt
     2221 gcgctcccga cgatcgtcga gtcgccacgc tccatgctgg ttcgaacggg ccagaccgcc
     2281 agcttgtccg caacggcttc gggccttcca gctccgacgt tgcaatggca gtcacgcccc
     2341 gccaattcta cgggcgcctg gagcaacgtg accaccggca ccggcgccac ggctgctaac
     2401 tacaccaccg cggcactggt gccgggtgac aacggaactc agtaccgcat cgtcgccacc
     2461 aatgcggttg gcgttgccga gagttccgca gtcacggtgt cggtcagcga cctcgatgtc
     2521 gcgccgacca ttgcgacaca acccgccagc ctgagcgtcg cgtccggcag cgacgcgatc
     2581 ttcgcggtcg atgcgcgcgg caccgaagcg ctgagttacc agtggttccg caatggcgtg
     2641 gcgatcgctg gcgccaacag tcccgtgttg cgcctcccgg cagtaacgga cgcgaatgcc
     2701 ggcagctaca gcatcggggt gagcaacagc gcgggaaatg ccaccagcaa cgcggccgtc
     2761 ctgaccgtgt ccgccagcgc gcccgccgca gtcgccccat cgatcgtcac gcagccggtg
     2821 agcgtcaccg ccaacgtcgg cagcaccgcc acgctcgcgg tcggtgtcga tggcaccggg
     2881 ccgttcacgt tccagtggcg acgcgatggc gtcaatgtca cgggcgcgac cagcgccgtg
     2941 ctgaccttcc ctgccgtcgc tctacccaac gccggaagtt tttcggtgct ggtaaccaac
     3001 agcgccggct ccgtcaccag cggcaatgcg gtgctcgacg tccgcgggga ttccaccgcg
     3061 accattccca ccatcacctc acaaccgtcc acgctcatcg tgcccttccg cgggtccggt
     3121 gtgctcgcgg tgggcgccac cggcagcggc ccgctgtcct atcaatggtc caaggatggc
     3181 gcggagcttc ccggcgcgac attgcccgtg ctcgacttcg caatcgtggc cgacgtggat
     3241 gtgggcaggt atacggtgac cgtgtccaac aacgtgggct cggtggtgag tagtgcggct
     3301 gacatcatcc tgctcggtgc accggtgatc acgcagcagc ccacggacgt gacagctctc
     3361 gagggcggca acgccatgtt cttcgtggcc gccagcagct cgggcctgcg atatcaatgg
     3421 tcagccaatg gcaatcccat ccccggcgcc acggccgcca ccttgaacct gaactcggtg
     3481 gtgctggcca acaacgcctc ggtc
//
LOCUS       DATDEU010000147         7075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_463790, whole genome shotgun sequence.
ACCESSION   DATDEU010000147 DATDEU010000000
VERSION     DATDEU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463790"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VIV63_03675"
     CDS             <1..806
                     /locus_tag="VIV63_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6123725.1"
                     /translation="GETIPAIGLGTSGPFEVGADDASRAPLREVLKEFFASGATLIDT
                     SPMYSTAETVLGDLLTADQQAKAFIATKVWTPSSGGHGEQKGIEQMQRSMALLKHRRI
                     ELMQVHNLVDLDAHLKTLRRWKAEGLIKYIGVTHYTTSSYPDLIAVIEREKIDFVQFN
                     YSVLTRDAEKRLLPLCADRGVAVLINRAFEDGSLFSKVQGKALPPWAAEFGAGSWAQV
                     FLKFVLAQPAVTCVIPATGKLRNLVDNLAAGMGALPDAKQRAQIVASLS"
     gene            complement(803..2335)
                     /locus_tag="VIV63_03680"
     CDS             complement(803..2335)
                     /locus_tag="VIV63_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY6123726.1"
                     /translation="MNRSVVLAALASFGLLLSACGGGGGDSPPPQNPPPPAADSTAPT
                     VPGGVTAVAQSASSVLVSWTASTDASGISGYRVFRDGSTTPLATVQTTTYTDTGLAAS
                     TSYSYTVAAVDGAATPNQSAVSTAAAATTQAAAPARIAAQRVFSGLTLFAAHSLLRVP
                     HDTSRWVVVQQDGHVVAFADTPNVTSTSNVLDISDRVVFRNVHGLLGLAFHPNYPTDT
                     RAYAAYTHEVSAGVIVLRISEFNTADGGATLSAASEQIMFEMAQPGGHNNGGHLLFGP
                     DGFLYLGIGDGGNDDSASGVAGNGQLTNNLLGKILRVDVSGTLGPRRYRIPADNPFAA
                     NPMCNLDGTGAQDCPEIFAWGFRNPWRWTFDRQGGQLWLGDVGSHTREEVNRVVRGGN
                     YGWRCFEGTLQTALNCGTPSTPLLPPVAEYIHPTGQSVVGGFVYRGTAIPALAGRYVF
                     ADYNLGYFWNIAADTAPTRTMVIADAWASGLNPASFAEDNDGELFFVDVRTGSIYKIV
                     AG"
     gene            complement(2494..3147)
                     /locus_tag="VIV63_03685"
     CDS             complement(2494..3147)
                     /locus_tag="VIV63_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY6123727.1"
                     /translation="MSNALPALIFDCDGVLIDSEYLASRVESELVGALGLTLGVEEAH
                     DLFLGKTVDGVLDAIASRTGQRPTAAWVYNWAFATAHTFLRELKSVDGVGVAVETLRR
                     RGHRMAVASQSPLARVQLSLHVAGLAGQFGEHIYVTSMVARPKPSPDIYLLAAQKLGV
                     EPHDCIVIEDSPASAAAALGAGMRVIGYAPDKTFDAMRASGATVIRTMDELIPTIGT"
     gene            complement(3155..4015)
                     /locus_tag="VIV63_03690"
     CDS             complement(3155..4015)
                     /locus_tag="VIV63_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEY6123728.1"
                     /translation="MAFTNNVFRGPGNSGSGGVELPIRKIITWAVVLVVAIIVVSVAG
                     CGIKVVDTGQRGVKTRFGEITSTSLPEGLYFFNPITSKIIEIDTRVKRTDGETESYTR
                     DVQQANIKYTLNYRLQQNQAHTMYRDVGTDWEQRLIPQVILGTLKEVVGKWDAVDLIS
                     NRDKATTTAFEQIRSDLAERFVEVSRFEITDISYTTEFETSVEQKVIAQQKAIEEQNR
                     TKQIEEQARQKVLSAEAEAKSMQIRAEALEQNAKLVEWEAVQKWNGVLPQYMLGSGAT
                     PFINLNPGKN"
     gene            complement(4038..4244)
                     /locus_tag="VIV63_03695"
     CDS             complement(4038..4244)
                     /locus_tag="VIV63_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123729.1"
                     /translation="MLFALLRLVGVIWIFGFIVLALVATSTFLYSTEDSAERNQRWQT
                     RLRMALIWPIALMSPAGRARLRRG"
     gene            complement(4253..5002)
                     /locus_tag="VIV63_03700"
     CDS             complement(4253..5002)
                     /locus_tag="VIV63_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY6123730.1"
                     /translation="MEAIVALLTDPQVWIAFFTLTALELVLGIDNIIFISILVDKLER
                     EQREKARRIGLFLAMFMRVGLLLVLSWIVGLTEPLFTITGTEISGRDLILIAGGIFLV
                     WKSTMEIHQLTEGEEGHASKSVKASFGAIIVQIIIIDMVFSLDSIITAVGLVDQVSVM
                     IAAVIASVGLMMLFARAIGDFVSAHPSIKMLALSFLLLVGVMLVAEGFDHKVPKGYIY
                     FAMAFSVAVEMLNIRMRKKKSPPVNLHEHYK"
     gene            complement(5027..5905)
                     /locus_tag="VIV63_03705"
     CDS             complement(5027..5905)
                     /locus_tag="VIV63_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123731.1"
                     /translation="MKRVRPRMTAAMAMAAALAFACVAQAVPLTANLSVSADDAGGTA
                     IDADFTVSPTEHLTLMAGAGHSESPARTVELRGTLLNAGASLYGDRAGVALGYDLFDD
                     NTSYHAGTLGARAWVRAGDFEFTLLARHRDMGVELTLDLPLRTVRREVEFSALGAGAQ
                     IAFSRGDFHVYAMTIGYDYDEQFDEFLDLAGSPLLDERPRIEALLGSFLTQAQGAIDR
                     QTGAGVEYMSGRHSLAVDFSYVHDAVLDSSSTSVVLTFSRAESAHLDWSLSAGMADSE
                     AYGGIAFLGVSFGLAN"
     gene            5988..>7075
                     /locus_tag="VIV63_03710"
     CDS             5988..>7075
                     /locus_tag="VIV63_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HEY6123732.1"
                     /translation="MVKTVVRILSAIVLLSFAPVSQARLLSGEVRVWEVQEITLAAAR
                     DYANPYADIECGVELEGPDFRRRVHGFWDGGRTFKVRVVATHPGEWTWRVGANRDDPG
                     LQGSGKFRAVAWNETDLARNPNRRGFVRNSENGHALRYADGTPFFLTGDTWLAASTWR
                     LPFKATRAADGYVPAEGISFEEAVAWRKRQGYNSISFIAAFPNWAADEHGATFANKDG
                     VYLRNAWEKFGHWAPNAKISTADGATTTAKDMHDEQGNRPFEIMADREGMANFDRINP
                     KYFASLDRKMRHLSDEGFVPFFETIRRDNAPSWKRYFDFNASYARFVQYLIARYGAYN
                     MVFSGIHLDWIPKDFSLTADEFNEALTW"
BASE COUNT         1305 a         2238 c         2298 g         1234 t
ORIGIN      
        1 cgggtgaaac cattccggcc atcggtctcg gcacgtcagg tcctttcgaa gtgggcgccg
       61 acgatgcttc ccgggccccg ctgcgcgaag tgttgaagga attcttcgcc agtggcgcca
      121 cgctcatcga cacctcgccc atgtattcca cggccgaaac cgtgctcggc gacctgctga
      181 ccgcggacca gcaggcaaag gcgttcatcg ccaccaaggt gtggaccccg agctcgggtg
      241 gccacggcga gcagaaaggc atcgagcaga tgcagcgttc gatggcgctg ctcaagcaca
      301 ggcgcatcga gctcatgcag gtacacaacc tcgtcgatct cgacgcgcac ctcaagacgc
      361 tgcggcgctg gaaggccgaa ggcctcatca agtacatcgg cgtcactcac tacacgacca
      421 gttcctatcc cgacctcatc gccgtcatcg agcgcgagaa gatcgatttc gtgcagttca
      481 actactccgt gctgacgcgt gatgccgaga agcggctgct gccgctgtgc gccgaccgcg
      541 gcgttgcggt gctcatcaac cgcgctttcg aggacggcag cctgttcagc aaggtgcagg
      601 ggaaggcttt gccgccttgg gcggcggagt tcggcgccgg aagctgggcg caggtgttcc
      661 tcaagttcgt tctggcgcag ccggccgtca cctgcgtgat cccggccacc ggcaagctca
      721 ggaatctggt cgacaacctg gccgcgggaa tgggcgcgct gcccgacgcg aagcagcgcg
      781 cgcagatcgt ggcttcgctg agctagcccg ccacgatctt gtagatagac ccggtgcgca
      841 cgtcgacgaa gaacagttcg ccgtcgttgt cttcggcaaa tgatgccgga ttcagccccg
      901 aagcccacgc atcggcgatc accatggtgc gcgtcggcgc ggtgtcggcc gcgatgttcc
      961 agaaataccc gaggttgtag tcggcgaaca cgtaacggcc ggcgagcgcg ggaatcgcgg
     1021 tgccgcgata cacgaaaccg ccgaccaccg actgcccggt cggatggatg tactcggcca
     1081 cgggcggaag cagcggcgtc gacggcgtgc cgcagttgag cgcggtctgc agggttcctt
     1141 cgaagcagcg ccagccgtag ttgccgccgc ggacgacgcg attcacttcc tcgcgcgtgt
     1201 gtgaccccac gtcgccgagc cagagttgtc cgccctggcg gtcgaaggtc cagcgccacg
     1261 gattgcggaa gccccaggcg aagatctccg ggcagtcctg cgcaccggtg ccgtccaggt
     1321 tgcacatcgg atttgccgcg aacggattgt ccgccgggat gcgataccgg cgagggccga
     1381 gcgtcccact cacgtccacg cgcaggatct tgccgagcag attgttggtg agctggccat
     1441 tgccggcgac accactggcg ctgtcgtcgt tgccgccatc accgatgccg aggtacagga
     1501 agccatccgg cccgaacagc aggtgtccgc cgttgttgtg tccgccgggc tgcgccatct
     1561 cgaacatgat ctgctcggac gcggcgctca gcgtggcgcc gccatcggcg gtgttgaatt
     1621 cggagatccg cagcacgatg acgccggcgc tcacctcgtg cgtgtacgcg gcgtaggccc
     1681 gcgtgtcggt ggggtagttc gggtgaaacg ccagacctaa caagccatgg acgttgcgga
     1741 acaccacgcg gtcgctgatg tcgagcacgt tgctggtgga ggtcacgttc ggcgtgtcgg
     1801 cgaaggccac gacgtggccg tcctgctgta cgaccaccca gcggctggtg tcgtgcggaa
     1861 cacgcaacag cgagtgcgcg gcgaacagcg tgagaccgga gaagactcgc tgcgcggcga
     1921 tgcgggcggg cgcggcggcc tgggtcgtcg cggcggccgc ggtggacacg gccgactggt
     1981 taggagtcgc ggcgccatcg acggccgcca cggtgtagct gtagctggtg ctggcagcga
     2041 gccccgtatc ggtgtacgtc gtggtctgga cggtggcgag cggtgtggtg gaaccgtcgc
     2101 ggaacacccg atacccggag atgccactgg catccgtcga ggcagtccag ctcacgagga
     2161 cggaacttgc cgactgcgcc accgcggtca cgccaccggg gaccgttggc gccgtgctgt
     2221 cggcggcggg cggcggaggg ttctgcggtg gcggactgtc tcctccgcct ccgccacagg
     2281 cagacaacag gaggccgaaa gacgcgagcg ccgcgagcac cacagaccga ttcattccga
     2341 ttcccgataa tagtttttgg acaggggcgg tgaatctgac gcccgtcccg tcttcgcgcc
     2401 gtcagacagg gcgcgcacac acgtgcgcgc ggctggagac cgcaggggga atccgcggaa
     2461 atcgcgtgta ggagagcgcc gctcggcgcg gcttcaggtc ccgatggtgg gaatcagctc
     2521 gtccatcgtc cggatgaccg tagccccgct cgcacgcatg gcgtcgaacg tcttgtcagg
     2581 cgcgtaaccg atgacccgca tgccggcgcc gagcgcggca gcggcgctgg cgggggaatc
     2641 ctcgatgacg atgcagtcgt gaggctcgac tccgagcttc tgcgccgcca gcagatagat
     2701 gtccggcgac ggtttgggcc gggccaccat cgaggtgacg tagatgtgtt caccgaactg
     2761 gccggcgagc cccgcgacgt gcagcgaaag ctgcacgcgc gcgagcggcg attgcgaggc
     2821 caccgccatg cgatgaccgc ggcggcgcag cgtttcgacc gccacaccca cgccatcgac
     2881 cgacttcaac tcgcgcagaa aagtatgggc cgtggcgaac gcccagttgt atacccaggc
     2941 ggcagtcggg cgctgtccgg tacgcgacgc gatagcgtcg agcacgccat cgaccgtctt
     3001 tccgaggaac aggtcatgcg cttcctcgac gccgagcgta aggccaagcg cgccgaccag
     3061 ctcggattcg acgcggctcg ccaggtattc actgtcgatg agcacgccat cgcagtcgaa
     3121 gatcagcgcg ggcagtgcgt tgctcaaact actctcagtt cttgccggga ttgaggttga
     3181 tgaaaggcgt ggcgccgctg cccagcatgt actgcggcag gacgccgttc catttctgca
     3241 ccgcttccca ctcgaccagc ttggcgttct gctcgagcgc ctcggcgcgg atctgcatgg
     3301 atttggcttc cgcctcggcc gacagcacct tctggcgcgc ctgctcctcg atctgcttgg
     3361 tgcggttctg ttcttcgatc gctttctgct gcgcgatcac tttctgctcg accgaggtct
     3421 cgaactccgt ggtgtacgaa atgtcggtga tctcgaagcg ggagacttcg acgaaccgct
     3481 cggcgagatc cgagcggatc tgttcgaacg cggtggttgt cgccttgtcg cgattggaga
     3541 tgagatcgac cgcgtcccac ttgcccacga cttccttcaa ggtgccgagg atcacctggg
     3601 gaatgagccg ctgctcccag tcggtgccca cgtcgcggta catggtgtgc gcctggttct
     3661 gctgcaggcg gtaattgagc gtgtacttga tgttcgcctg ctgcacgtcg cgcgtgtacg
     3721 actcggtttc gccgtccgtg cgtttcacgc gcgtgtcgat ctcgatgatc ttgctggtga
     3781 tcgggttgaa gaaatagagc ccttcaggca gcgacgtgga agtgatctcg ccgaaacgtg
     3841 ttttcacgcc gcgctggccg gtgtccacga ccttgatgcc gcaacccgcg acgctgacga
     3901 cgatgatggc gaccaccagc accaccgccc acgtgatgat cttgcggatg ggtagttcga
     3961 cgccgccgct gcccgaattg cccggaccgc ggaatacgtt gttcgtgaaa gccattgtcg
     4021 attctccctg gaactgatta accccggcgc aggcgtgcac gcccggccgg tgacatgagc
     4081 gcgatcggcc agatgagcgc catgcgcaat cgcgtttgcc agcgctgatt gcgctcggcg
     4141 gaatcttcgg tcgagtagag gaacgtcgac gtcgcgacca gcgcgagcac gatgaatccg
     4201 aagatccaga tgacgccgac gagacgcaac agggcgaaca gcatggctga ccctacttgt
     4261 agtgttcgtg cagattgacc ggcgggctct tcttcttgcg catgcggatg ttgagcattt
     4321 ccactgcaac cgagaacgcc atggcgaagt agatgtaacc cttcggcact ttgtggtcga
     4381 agccttcggc gacgagcatg acaccgacga gcagcaggaa agacagcgcc agcatcttga
     4441 tggagggatg tgccgacacg aaatcgccga tggcgcgcgc gaacagcatc atgaggccga
     4501 ccgaggcgat caccgccgcg atcatgaccg acacctggtc gaccaggccg accgcggtga
     4561 tgatggaatc gagcgagaac accatgtcga tgatgatgat ctgcacgatg atggcgccga
     4621 agctggcctt cacgctcttg gaggcatgac cttcctcacc ctcggtgagc tggtgaatct
     4681 ccatggtcga cttccacacc aggaatatgc cacccgcaat gaggatgagg tcgcggccgg
     4741 agatttccgt gccggtgatg gtgaacagcg gctcggtgag ccccacgatc cacgacaaaa
     4801 ccagtagcaa cccgacacgc atgaacatgg cgaggaacag gccgatgcgg cgcgccttct
     4861 cgcgctgctc gcgctcgagc ttgtcgacca ggatcgagat gaaaatgatg ttgtcgatgc
     4921 cgagcaccag ctcgagcgcc gtcaacgtga aaaaagcgat ccatacctgc ggatcggtca
     4981 ggagggcgac aatggcttcc atgggtggcc gccatattag atgaaatcag ttcgcgaggc
     5041 cgaagctcac gccgagaaag gcgatgcccc cgtaggcttc gctgtccgcc atgccggctg
     5101 atagcgacca gtcgaggtgg gccgattccg cacggctaaa ggtgagcacg acgctggtgc
     5161 tgctcgagtc cagcacggcg tcatgcacat atgaaaagtc caccgccagc gaatgccgtc
     5221 cagacatgta ttccacgcca gcgccggtct ggcggtcgat ggcgccctgg gcctgcgtca
     5281 gaaacgaccc gagcagcgct tcgatgcgcg gccgttcgtc gagcagcggc gaccccgcga
     5341 ggtcgaggaa ctcatcgaac tgctcgtcgt agtcgtagcc gatggtcatg gcatagacgt
     5401 gaaagtcgcc acgcgaaaac gcgatctgcg ccccggcgcc cagtgcggaa aattcgacct
     5461 cgcgccgcac cgttcgcagc ggcaggtcga gcgtgagctc gacgcccatg tcgcgatggc
     5521 gcgcaagcag cgtgaactcg aagtcacctg cccgcaccca ggcgcgggcg cccagggttc
     5581 cggcgtggta gctagtgttg tcgtcgaaga ggtcgtagcc cagcgcgacg cctgcgcggt
     5641 cgccatacag ggaagcgcct gcgttgagca gcgtgccgcg cagctcgacg gtcctggcgg
     5701 gactctccga atgcccggcg cccgccatca gcgtgaggtg ttcggtgggc gagacggtga
     5761 aatcggcgtc gatcgccgtt cctcccgcgt catcggccga aaccgaaaga ttcgcggtga
     5821 gcggcaccgc ctgcgcgacg caggcaaacg ccagcgcggc ggccatggcc attgccgccg
     5881 tcatacgcgg acgtacccgt ttcatgaaaa ctccctgagc cgatccttct gcgtacaacg
     5941 cgcttaggtc taatgggttt acgccgtgaa ggaggtacgc ttccgttatg gtcaaaaccg
     6001 tcgtccgcat cctctcggcc atcgtgctgc tttcattcgc gccggtctcg caggcgcggc
     6061 tgctctccgg tgaagtgcgc gtctgggagg ttcaggaaat aacgctcgcg gccgcgcgcg
     6121 actacgcgaa cccctatgcc gacatcgaat gcggggtgga gctcgaaggc ccggatttcc
     6181 ggcggcgtgt ccatggattc tgggacggcg gccgcacgtt caaagtgcgc gtggtcgcga
     6241 cacatccggg cgaatggacc tggcgcgtgg gtgcgaaccg cgacgatccc ggccttcagg
     6301 gatcgggaaa attccgcgcc gttgcctgga acgagaccga ccttgcgcgg aatcccaatc
     6361 ggcgcggctt cgtgcgcaac agcgaaaacg gccacgcgct tcggtacgcg gacggcacac
     6421 cgtttttcct cacgggtgat acctggctcg ccgcctcgac ctggcgtctg ccattcaagg
     6481 caacgcgcgc tgccgacgga tatgtgccgg ccgaaggcat cagcttcgaa gaagccgtag
     6541 cctggcgcaa gcgccagggc tacaactcga tctcgttcat agccgcgttt ccgaactggg
     6601 ctgcggacga acacggcgcg acgttcgcca acaaggacgg cgtctatctg cgcaacgcct
     6661 gggaaaagtt cgggcactgg gcgccgaatg cgaagatttc cacggccgat ggcgccacca
     6721 ccaccgccaa ggacatgcac gacgagcagg gcaaccgacc tttcgagatc atggccgacc
     6781 gcgaaggaat ggcgaacttc gatcgcatca atccgaagta tttcgccagc ctcgatcgaa
     6841 agatgcgcca cctctccgac gaaggattcg ttccgttctt cgagaccatt cgccgcgaca
     6901 acgcgccatc ctggaagcgc tatttcgact tcaacgcgtc ctacgcgcgc ttcgtgcaat
     6961 acctcattgc ccgctacggc gcgtacaaca tggtgttcag cggaattcac ctcgactgga
     7021 ttccgaagga cttcagcctc acggccgacg agttcaacga ggcgctgacc tggca
//
LOCUS       DATDEU010000148         2989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1338943, whole genome shotgun sequence.
ACCESSION   DATDEU010000148 DATDEU010000000
VERSION     DATDEU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338943"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VIV63_03715"
     CDS             complement(<1..229)
                     /locus_tag="VIV63_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6123733.1"
                     /translation="MKFSSYFIDRPVFASVLSAFILIAGAISLFKLPISEYPEVVPPS
                     VVVRAAYPGANPKVISETVAAPLEQAIVGVED"
     gene            complement(242..1426)
                     /locus_tag="VIV63_03720"
     CDS             complement(242..1426)
                     /locus_tag="VIV63_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6123734.1"
                     /translation="MPASNKTSIAITLMTPLAAMILASCARNEAADAPAAPPTVQAAK
                     VVSKSITEFDEFTGRFEAVERVEVRPRVSGYVMSTRFAQGHEVRKGDILYVIDARPYQ
                     ATLKHAQAELTRARTQAALAESERVRATKLIEKRAISQEEFDARTSGTEQAAANLQAA
                     EAAVESAALDLSFTEVRAPISGLVGRAEITAGNLVSGGQTLLTTVVSIDPIYVSFDGD
                     EQVYLKNVGMELRGETKSQRNTQNPVWAGLADEQGHPHEGVMVFLDNELDPATGTIHA
                     RGLFKNADRRFTPGMFARVKLIGSGKYDALLINDSSIGTDQSVKFVLRIGADNKIEYT
                     PVKLGPLVDGLRVVREGLKANDVIVVKGLQQVRPGMPVTPQLVAMGEPRRGGNNTMVA
                     QN"
     gene            1587..2102
                     /locus_tag="VIV63_03725"
     CDS             1587..2102
                     /locus_tag="VIV63_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY6123735.1"
                     /translation="MELVLQAAAFAAEKHRNGRRKDVEASPYINHPIQLAYILVQADV
                     EDPIVLAAALLHDTIEDTKTTHDEIEIVFGHEIANIVAECSDDTTLDKLVRKQAQVDR
                     AAHISKRAKLVKLADKIANVSDIDGAPPAGWSLERKREYFDWAKRVVDKMRGVHPKLE
                     ARFDAEYAKRP"
     gene            2099..2731
                     /locus_tag="VIV63_03730"
     CDS             2099..2731
                     /locus_tag="VIV63_03730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY6123736.1"
                     /translation="MTPVVRLLVFANLAVFAIQQMVRDELLTHFALWPLGSHFIAGFG
                     RVGFEPWQLITSSFLHGGVTHIALNLYALYAFGGMVERVLGTRRFALLYFASVLAGSL
                     VQLLVVTATLDQGVAPTVGASGGVFGVLLAFAALFPHSRVMLVFPPIPMKAWVLVAGY
                     AAVELMLGVFGSNQGVAHFAHLGGMLGAVLVLLAIGFRRRVQPRGDVRGP"
     gene            complement(2672..>2989)
                     /locus_tag="VIV63_03735"
     CDS             complement(2672..>2989)
                     /locus_tag="VIV63_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123737.1"
                     /translation="GDKITRRAFGDVMHGDYIAQEFSPPGERRRGSSPDCFKLDVRSY
                     AYDGVQQMLAARLYQGQTTNFRSAGGGFAPVYVLDEAGSQESSGSADVATRLHAPAET
                     DRQ"
BASE COUNT          481 a          943 c         1001 g          564 t
ORIGIN      
        1 tgtcctcgac gccgacgatg gcctgttcga ggggcgcggc cacggtctcg gagatgacct
       61 tgggattggc gcccgggtag gccgcgcgca cgaccaccga cggcggcacc acttccgggt
      121 actcgctgat cggtagtttg aacagcgaga tggcgccggc gatgaggatg aaggccgaca
      181 ggaccgatgc gaagaccggg cggtcgatga agtagctgga gaatttcatt gcaggatccg
      241 tttagttctg cgcgaccatc gtgttgttgc cgccgcggcg cggctcaccc atggcgacga
      301 gctgcggcgt gaccggcatg cccgggcgca cctgctgcag gcccttcacg acgatgacgt
      361 cgttggcctt caggccctcg cgcacgacgc gcaggccatc gacgagcggc cccaacttca
      421 cgggcgtgta ctcgatcttg ttgtccgcgc cgatgcgcaa cacgaacttc acgctctggt
      481 cggtgccgat ggagctgtcg ttgatgagca gcgcgtcgta cttgccgctg ccgatgagtt
      541 tcacgcgcgc gaacattccg ggggtgaaac gccgatcggc gttcttgaac aatccgcgcg
      601 cgtgaatggt gccggtggcg gggtcgagct cgttgtcgag gaagaccatg acaccttcgt
      661 gcgggtgacc ctgctcatcg gcgaggccgg cccacacggg gttctgggtg ttgcgctggc
      721 tcttggtctc gccgcgcagt tccatgccca cgttcttgag atacacctgc tcgtcgccgt
      781 cgaacgagac gtagatggga tcgatcgaca ccaccgtcgt cagaagcgtc tgaccgccgg
      841 agacgaggtt gcccgcggtg atttccgcgc ggcccacgag acctgagatc ggcgcgcgca
      901 cctcggtgaa cgacagatcg agcgccgcgg attccacggc cgcttcggcc gcttgcagat
      961 tcgctgccgc ctgttcggtg cccgaagtgc gcgcgtcgaa ttcctcctgg gagatcgcgc
     1021 gtttctcgat gagcttggtg gcgcggacgc gctcggactc ggccagcgcg gcctgggtgc
     1081 gggcgcgcgt gagttcggcc tgcgcgtgtt tcagcgtcgc ctggtagggg cgcgcgtcga
     1141 tgacatagag aatgtcgcct ttcctgacct cgtggccctg cgcaaagcgc gtggacatca
     1201 cgtagcccga cacccgcggc cggacctcga cgcgttcaac cgcctcgaag cggcccgtga
     1261 actcgtcgaa ctcggtgatc gatttggata caaccttggc ggcctgcacc gtcgggggcg
     1321 cggcgggcgc atcggcggct tcgttgcggg cgcaggaggc gaggatcatc gcggctagtg
     1381 gtgtcatgag cgtgatggca atcgaagtct tgttggatgc aggcacagaa gtctcccgtc
     1441 aggttgtggg acgcatcttc cgcgcatgga gctccgccga acattcaccg ggtgaggcac
     1501 ggcgcgggtc caattcggaa cggagtgcgc tatccctttc gggggaggct gctttgcggg
     1561 tacattcgcc cgcgatgcca actcatatgg aactggtact gcaggcggcg gcgtttgccg
     1621 ccgagaaaca ccgcaatggc cgccgcaaag atgtggaggc ctcgccgtac atcaaccacc
     1681 cgatccagct cgcctacatc ctggtgcagg cggacgtcga agatcccatc gtgctggccg
     1741 ccgcgctgct gcacgatacg atcgaagaca ccaagacgac tcacgatgaa atcgaaatcg
     1801 tgttcggtca cgaaattgca aacatcgtgg ccgagtgttc cgacgacacg acgctcgaca
     1861 agctggtgcg caagcaggcg caggtcgatc gcgcggcgca catcagcaag cgcgcaaagc
     1921 tcgtgaagct tgccgacaag atcgccaacg tgagcgacat cgatggcgcg cctcccgccg
     1981 ggtggtcact cgagcgcaag cgcgaatact tcgactgggc caagcgcgtc gtcgacaaga
     2041 tgcgcggcgt gcatccgaaa ctcgaagccc gtttcgacgc ggagtacgcg aagcgtccat
     2101 gacccccgtc gttcgcctgc tcgtgttcgc caacctcgcc gtattcgcca tccagcagat
     2161 ggttcgcgat gagctgctga cacacttcgc gttgtggccg ctcggctcgc acttcattgc
     2221 aggcttcggc agggtggggt tcgagccctg gcagctcatc acctcgtcgt tcctgcatgg
     2281 cggcgtcacg cacatcgcgc tcaatctgta cgcgctgtat gcgttcggcg gcatggtcga
     2341 gcgcgtgctg ggcactcgcc ggttcgcgct gctgtacttc gcctcggtgc tggccgggag
     2401 tctcgttcaa ctgctcgtgg tcacggccac gctcgatcag ggagtggcgc ccacggtggg
     2461 cgcatcgggc ggcgtgttcg gcgtgctgct ggcattcgcg gcgctgttcc cacatagccg
     2521 cgtgatgctg gtgttcccgc ccatcccgat gaaggcctgg gtgctcgtgg ccggttatgc
     2581 ggccgtcgaa ctcatgttag gcgtgttcgg ctcgaatcag ggcgtggcgc acttcgcaca
     2641 tctcggcgga atgctgggcg cggtcctggt gttactggcg atcggtttcc gccggcgcgt
     2701 gcagccgcgt ggcgacgtcc gcggaccctg acgactcctg cgatccggct tcgtcgagta
     2761 cgtacaccgg cgcaaatccg ccgcccgccg aacggaagtt cgtggtctgg ccctggtaca
     2821 ggcgagcggc gagcatctgc tgaacgccgt catacgcgta gctgcgcaca tcgagtttga
     2881 aacagtcggg cgacgatccg cgccggcgct ctccgggtgg cgagaactcc tgcgcgatgt
     2941 agtccccgtg catgacgtcg ccgaacgcgc gccgcgtgat cttgtctcc
//
LOCUS       DATDEU010000149         6326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_464018, whole genome shotgun sequence.
ACCESSION   DATDEU010000149 DATDEU010000000
VERSION     DATDEU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464018"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1193
                     /locus_tag="VIV63_03740"
     CDS             120..1193
                     /locus_tag="VIV63_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEY6123738.1"
                     /translation="MNIARFVLLGLLLLPTANAAELDAWSAAKLMGVGANIGNTLENT
                     TTWETGWGNPRITKEFVENLAKLGFKTVRVPVAWDTYAHDGRVDAAKIARVAEVVEWI
                     TAAGMFCVINIHWDGGWIDSSAKERFPKTFATFSAEAEQKFVSYWTQIAHHFAGTSER
                     VIFEALNEETNFEGAGSARKAHATLTRVNQLFIDTVRKTGDNNANRLLIVTGYATDIT
                     KTTSEDFALPTDTVPHRLLISVHYYTPWQFAGMTEDASWGKMQATWGSASDVSELNRL
                     FDAMQEFCKRHDLPAFIGEFGVTDKKETASRVRWMAAVAEAALSRKMVPVLWDTGGDL
                     SRKPPYGASAAVSQVLEKLGIAR"
     gene            1203..5099
                     /gene="hrpA"
                     /locus_tag="VIV63_03745"
     CDS             1203..5099
                     /gene="hrpA"
                     /locus_tag="VIV63_03745"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEY6123739.1"
                     /translation="MNSPRPVPELSIDLCLRRDQHRLRREIDRLRGDKLRGKDVAAAE
                     ATLTQKIAASAAARAARAASVPAIAYPEELPISQNRDEIRRAIEKHPVVIVCGETGSG
                     KTTQLPKICLEAGRGVAGIIGHTQPRRIAARAVASRIAEELKTPLGQLVGYKLRFQDH
                     SRPEGLIKLMTDGILLAETQGDRFLDSYDTIIIDEAHERSLNIDFLLGYLKYLLPKRP
                     DLKLVITSATIDPERFSRHFSDAPIINVSGRSYPVEVRYRPVELDEEDDETASVEQDA
                     IISAVDELWREQAGDILVFLSGEREIRETAESLRKHHPQGCEILPLYSRLSQEEQQRV
                     FRPSGRRRVVLATNVAETSLTVPGIRAVIDTGVARISRYSHRTRLQRLPIEKISQASA
                     NQRSGRCGRVGPGVAIRLYSEEDFAARPAFTEPEIQRTNLAAVILQMHALKLGDIEAF
                     PFVEPPDGRFVRDGQRTLRELGALSEEGTLTDTGRQLAKLPLDPRLGRMLLAAAAEHC
                     LEEVAIIASALSVPDPRDRPADKQTQADQKHAPLRDEQSDFLSLLKLWREYVKQREHL
                     TRAKLRGWCKENFLSYLRLTEWHDVHGQVMEVVKGELAMKLNAQPGEYASIHRALLAG
                     LLSQVANRREQGEYLGANGSKIFIHPGSGQFKARPAWIVSAEQVETTKVYARNVARVE
                     PAWIEQAGAHLVKRQHYEPHWERRASRAAIYERVTLFGLLLSSGRRVPYEQIDPVAAR
                     ELFIKHALVHMEYDSRAPFLAHNLKLLEDTEYLQQKGRRVDLLGDETQLYAFFDARIP
                     PGISTGAAFERWRRDAEAKEPKLLWLTERDISPGDAELDAERFPDHLSAGPLVIQLRY
                     RFEPGHEDDGVTALIPLHVLNQLPEEAFEWLVPGLLDEKIEALVRSLPKNLRVHFVPV
                     PEAVARALPLLERGRGSLYAQLADALLRTGGVPVPPDSFREELLPPHLRMNFVLLDDA
                     DKVIARSRSLAALREKHAGASQQHYAKQSQITTGAKTWVFGDLPERQDVTQGGRAQLG
                     YPALVDEGDSVGLRVFATPAEARISHQRGCARLIRLVMARDFKSYRRDLPVNVQAEIV
                     YRALVPHPMLNPELVAGRDLREDLLDRIVMTVFLEGREPLRDSAAFDARIAELRGGLG
                     LPAQEISRHVQTALERLSRIQAALPKADPAAAADIRSQLAWLVPAGFLLNTPGERLRE
                     FPRYLQAIEQRLEKLGRDPRRDAQLAAEIAPLETRYRNRVKAERGLRPPGDDEFRWML
                     EEFRVSLFAQQLGTRSRISARRLEDAWVERERTQPA"
     gene            5129..5569
                     /locus_tag="VIV63_03750"
     CDS             5129..5569
                     /locus_tag="VIV63_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6123740.1"
                     /translation="MSTATDPVRDALSSYCAAAYDKDVERFVSLYAADLHVFDMWNAW
                     ELRGIDAWRNMAAGWFGSLGTERVVVTVADVASQVAGDLALGHATLTYTAVSDDGKEL
                     RSLDNRITIALRRTGSEWKIFHEHTSGPIDHQSMSGKIKRESTK"
     gene            5566..5958
                     /locus_tag="VIV63_03755"
     CDS             5566..5958
                     /locus_tag="VIV63_03755"
                     /inference="COORDINATES: protein motif:HMM:NF020457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEY6123741.1"
                     /translation="MNAADPWHAHIYYDDESWNSAQRLHQELSDLLMGGTLPDLRFVG
                     SMAETGVGPHPLPQFEIHFLASGLEQITRVLKASGLRTLIHPLTDDDLADHTTLAVWL
                     GEPLVLDESVLDPPGHNQGIARFGKSDF"
     gene            6019..>6326
                     /locus_tag="VIV63_03760"
     CDS             6019..>6326
                     /locus_tag="VIV63_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6123742.1"
                     /translation="MKLLFVEYASAIGIDLEYQGFSQELATLPGKYAPPSGELLLASI
                     DGVPAGCVALRALDGATVEMKRLYVRDAARGLGLGKRLVEAAIGAARTGGYAELRLDT
                     L"
BASE COUNT         1156 a         2082 c         2043 g         1045 t
ORIGIN      
        1 gagaccgagc tcaacttcaa gttctatcgc caggccacga acaagatcgt gggcatctcg
       61 gcggcggcgg gaaaatttct ggaccgctgt ttccagcgct gaacgggtag agtgcgcgca
      121 tgaacatcgc ccgattcgta ctgctcggcc tgcttctgct gcccaccgcc aacgccgccg
      181 agctcgatgc ctggagtgcc gccaaactca tgggtgtggg agcgaacatc ggcaacacgc
      241 tcgagaacac caccacctgg gaaaccggct ggggcaatcc gcgcatcacg aaggaattcg
      301 tcgagaacct cgcgaaactc ggattcaaga ccgtccgcgt gccggtggcc tgggacacct
      361 acgcgcacga tggccgcgtc gacgcggcaa agatcgcgcg cgtggcggaa gtcgtcgaat
      421 ggatcaccgc ggcaggcatg ttctgcgtca tcaacatcca ctgggacggc ggctggatag
      481 attcgagtgc caaagagcgc tttccgaaaa cgttcgcgac cttcagcgcg gaagcggagc
      541 agaagttcgt ttcctactgg acgcagatcg cccaccactt cgccggcacg agcgagcggg
      601 tgatcttcga ggcgctcaac gaggaaacca acttcgaagg tgcgggctcc gcgaggaagg
      661 cgcacgccac gctgacccgt gtgaatcaac tcttcatcga cacggtgcgc aagacgggtg
      721 acaacaacgc gaaccgcctg ctcatcgtca cgggctacgc gaccgatatc accaagacga
      781 ccagcgagga tttcgcgctg cccacggaca ccgtgccgca tcgcctgctg atctccgtcc
      841 actactacac gccgtggcag ttcgcgggca tgacggaaga tgcgagctgg ggaaagatgc
      901 aggccacgtg gggcagcgcc agcgatgtgt cagagctcaa ccggttgttc gatgcgatgc
      961 aggaattctg caagcgccac gacctgccgg cgttcatcgg cgagttcggc gtgaccgaca
     1021 agaaggagac ggcgtcgcgc gtacgctgga tggccgccgt ggccgaagcc gcgttgtcgc
     1081 gcaagatggt tccggtgttg tgggacaccg gtggcgacct ctcgcgcaag ccgccctatg
     1141 gcgcgagcgc cgcagtgagc caggtgctgg agaagctcgg cattgcccgg taatgcagaa
     1201 tcgtgaattc gccccgtccc gtccctgagc tctccatcga tctttgcctg cgccgcgacc
     1261 agcatcgcct gcggcgtgag atcgatcgac tgcgtggcga caagctgcgc ggcaaagacg
     1321 tcgccgcggc tgaagcgacg ctgacgcaga agattgctgc atcggccgcc gcgcgagccg
     1381 cgcgcgcggc gagcgtcccg gccatcgcct atcccgaaga gctgccgatc tcgcaaaacc
     1441 gcgacgagat tcgccgcgcc atcgagaaac atccggtggt catcgtgtgt ggcgagaccg
     1501 gctcgggcaa gacgacgcag ttaccgaaga tctgcctcga ggccgggcgc ggcgtcgcgg
     1561 gcatcatcgg ccacacgcag ccacggcgca tcgccgcgcg cgccgtcgcg tcgcgcatcg
     1621 ccgaagaact caagacaccg ctcgggcagt tggtcggata caagctgcga ttccaggatc
     1681 acagccggcc ggagggcctg atcaaactga tgaccgacgg catcctgctc gccgagacgc
     1741 agggtgatcg attcctcgat tcgtacgaca cgatcatcat cgacgaggcg cacgagcgct
     1801 cgctgaacat cgatttcctg ctgggctatc tcaaatatct gttgccgaag cggcccgacc
     1861 tgaagctggt catcacgtcg gcgacgatcg atccggagcg gttctcgcgg cacttcagcg
     1921 atgcgcccat catcaatgtg tctgggcgca gctatccggt cgaagtccga tatcggccgg
     1981 tcgaactcga tgaagaagac gacgagacgg cgtcggtcga acaggatgca atcatttcgg
     2041 cggtcgatga gctctggcgc gaacaggccg gcgacatcct ggttttcctg agtggtgagc
     2101 gcgagattcg cgaaacggcc gagtcgctgc gcaagcacca tccgcagggt tgcgagatcc
     2161 tgcctttgta ttcacgactg tcgcaggaag aacagcagcg cgtgttccgg ccgtcgggcc
     2221 gccggcgcgt cgtactcgcc accaacgtgg ccgaaacctc gctcacggtg cccggcattc
     2281 gcgcggtgat cgacacgggc gtcgcgcgca tcagccgcta cagccatcgc acgcgtctcc
     2341 aacggctgcc catcgagaag atttcacagg cttcggcgaa ccagcgttcc ggccgatgcg
     2401 gccgcgtcgg gccgggcgtg gcgattcgcc tgtattccga ggaagacttt gccgcccggc
     2461 ccgcattcac ggagccggag atccagcgca cgaatctcgc cgcggtgatc ctgcagatgc
     2521 acgcgctgaa actcggcgac atcgaggcct ttccgttcgt ggagccgccc gatggccgct
     2581 tcgtgcgcga cggccaacgc acgctgcgcg agctcggcgc gctgtcggag gaaggcactc
     2641 tcaccgacac cgggcggcag ctcgccaaac tacccctcga tccgcgtctc ggccgcatgc
     2701 tgctcgcggc cgccgccgaa cactgcctgg aagaagtcgc gatcatcgcc tcggccttgt
     2761 ccgtgcccga tccgcgcgac cggcccgcgg acaaacagac gcaggccgac cagaaacacg
     2821 cgccacttcg cgatgaacaa tcggacttcc tgtcgctgct caagttgtgg cgtgaatacg
     2881 tgaagcagcg cgagcacctg actcgcgcga aactgcgcgg atggtgcaag gaaaacttcc
     2941 tctcctatct acgactgacc gaatggcacg acgtgcacgg gcaggtcatg gaggtggtga
     3001 aaggcgagct cgcgatgaag ctcaacgcgc agccgggtga atacgcgtcc atccaccggg
     3061 ctctgctcgc gggtttgctc tcgcaggtcg cgaatcgccg ggagcagggc gaatacctcg
     3121 gcgccaatgg cagcaagata ttcatccacc cgggttccgg tcagttcaag gcgcggccgg
     3181 cctggatcgt gagcgccgaa caggtggaga ccacgaaggt ctatgcgcgg aacgtggcgc
     3241 gcgtcgagcc cgcgtggatc gagcaggcgg gcgcacatct ggtgaaacgc cagcattacg
     3301 agccgcactg ggagcgccgt gcgtcgcgcg ccgccatcta cgaacgcgtg acgctgttcg
     3361 gactcctgct gtcgtcgggc cgccgcgtgc cctacgagca gatcgacccg gtggcggcgc
     3421 gcgagctgtt catcaaacac gcgctggtcc acatggagta cgactcgcgg gcgccattcc
     3481 tcgcgcacaa cctcaaactc ctggaggaca ccgaatacct gcagcagaaa ggccgccgcg
     3541 tcgacctgct gggcgatgaa acccagctct acgcattctt cgatgcgcga attccgcccg
     3601 gcatctccac tggcgcggcg ttcgaacgct ggcgccgcga tgccgaggcg aaggaaccca
     3661 aactactgtg gctgaccgag cgcgacatct cgccgggcga cgccgagctg gacgcggagc
     3721 gctttcccga ccatctgtcc gcaggtcccc tcgtcatcca gctccgctat cgcttcgagc
     3781 cgggtcacga ggacgacggg gttaccgcgc tcattcccct gcatgtgctg aaccagcttc
     3841 ctgaagaagc gttcgagtgg ctggtgccgg gcctgctcga cgagaagatc gaggcgctgg
     3901 tccgctcgct gcccaagaat ctgcgcgtgc acttcgtgcc ggtcccggaa gccgtggcgc
     3961 gagcgctgcc gctgctcgaa cggggccgcg ggtcgctgta cgcgcagctc gccgacgcat
     4021 tgctgcgcac cggtggcgtg ccggtgccgc ccgattcgtt ccgcgaggag ctgctgccac
     4081 cgcacctgcg catgaacttc gtgctgctcg acgatgcgga caaggtcatc gcgcgctcgc
     4141 gcagccttgc cgcgcttcgc gaaaagcacg ccggcgcttc ccagcagcac tacgcgaagc
     4201 aatcgcagat caccaccggc gcgaagacct gggttttcgg cgatctgccc gaacgccagg
     4261 atgtgacgca gggtgggcgc gcgcagctcg gctatccggc gttggtggat gagggcgaca
     4321 gcgtgggctt gcgagtgttt gcgacgccgg ccgaggcacg catcagccat cagcgtggtt
     4381 gcgcacggct catccgcctc gtgatggcgc gcgacttcaa gtcgtatcgc cgcgacctcc
     4441 cggtcaacgt gcaggccgaa atcgtctatc gcgcgctggt tccacacccg atgctcaacc
     4501 cggagctcgt tgccggccgc gacctgcgcg aggacctgct cgatcgcatc gtgatgacgg
     4561 tattcctgga gggccgcgaa ccgctgcgcg acagcgccgc cttcgacgcg cgcatcgcgg
     4621 agttgcgtgg cgggctcggt ctgccggcgc aggagatttc ccgccatgtg cagacggcgc
     4681 tcgagcgcct gtcccggatt caggccgcgc taccgaaggc cgacccggct gcggccgccg
     4741 acatccgttc gcagctcgcc tggctcgtgc ccgcgggttt tctgctgaat acgcccggag
     4801 agcggctcag ggaattcccg cgctacctcc aggcgatcga gcagcggctc gagaagctag
     4861 gtcgcgatcc acggcgcgac gcgcagctcg ccgccgagat tgcgccgctc gaaacccgct
     4921 atcgcaaccg ggtcaaggcc gagcggggcc tgcggcctcc cggcgatgac gaattccgct
     4981 ggatgctcga ggaattccgc gtgtcgctgt tcgcacaaca gttgggcacg cgctcgcgta
     5041 tttcggcgcg gcgcctcgaa gacgcctggg tcgaacggga gcgaacgcaa ccggcttgaa
     5101 agccgccgcg cgggttatgc tccgcgccat gagcacagca acagacccgg ttcgggacgc
     5161 tctttcgtcc tactgcgccg ctgcctacga caaggacgtc gagcggttcg tgtccctcta
     5221 cgctgccgac ctgcatgtgt tcgacatgtg gaacgcgtgg gagcttcgcg gcatcgacgc
     5281 ctggcgcaac atggccgccg gctggttcgg atcgctggga accgaacgcg tcgtcgtgac
     5341 ggtggccgac gtggcatcgc aggtagccgg cgatcttgcg ctgggccacg ccactctcac
     5401 ctatacggcc gtttccgacg atggcaaaga gctgcgctcg ctcgacaacc ggatcacgat
     5461 tgcgttgaga cgcacgggca gcgagtggaa gattttccat gagcacacct ccggcccgat
     5521 cgaccaccag tcgatgagcg gcaagatcaa gcgcgagtcc acgaaatgaa cgcggcggat
     5581 ccctggcacg cccatatcta ctatgacgac gagtcgtgga attccgcgca gcgtctgcac
     5641 caggaactca gcgacctgtt gatgggcggc acgctcccgg acttgcgatt cgtgggcagc
     5701 atggccgaaa ccggtgtggg gccgcatccg ctgccgcaat tcgagattca ttttctggcg
     5761 tccgggctcg agcagatcac ccgtgtgctc aaggccagcg gcttgagaac gctcattcat
     5821 ccgctgaccg acgacgatct cgcggaccac acgacactcg cggtgtggct cggcgagccg
     5881 ctggtgctcg atgagagcgt gctcgatccg ccaggccaca atcagggcat cgcgcgtttc
     5941 ggaaaatccg acttctgagg ctggcccatg tcgttggctc aagtcacgcc ggcgagatca
     6001 gagacggact tgaccgtcgt gaaactgctt ttcgtcgagt acgcatcggc catcggcatc
     6061 gatctcgaat accagggctt cagccaggaa ctcgcgacgc ttcccggtaa atacgcgccg
     6121 ccctcgggcg agttgctcct cgcgtccatc gacggagtac cagcgggttg cgtggcgctg
     6181 cgcgcactcg acggcgccac cgtcgaaatg aaacgtttgt acgtgcgcga tgcggcccgt
     6241 ggcttgggcc tcggcaagcg gctcgtcgag gcagccatcg gcgccgcgcg cacgggcgga
     6301 tacgcggagc tgcggctgga cacgct
//
LOCUS       DATDEU010000150         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_464257, whole genome shotgun sequence.
ACCESSION   DATDEU010000150 DATDEU010000000
VERSION     DATDEU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464257"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VIV63_03765"
     CDS             <1..487
                     /locus_tag="VIV63_03765"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HEY6123743.1"
                     /translation="APAAAAATPPGMVTVPATSTYIPAGAAPAISTDPGVVTPSHTPP
                     LAEEPVLSGQEPSVPPDYDARDYRPAITPAQANAVAAARREMSAPSRDEVVAQGTQLP
                     DLRLDLHVYDPAPAKRFVFVNMRKLREGDSTPEGVRLDTITQTGAKFSFRGTDFTIEA
                     N"
     gene            complement(504..983)
                     /locus_tag="VIV63_03770"
     CDS             complement(504..983)
                     /locus_tag="VIV63_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6123744.1"
                     /translation="MLNIRPATRADAPVIANLVRELADYEKLLDEAKATPQDFLRELE
                     SPNPVIHVLIAEWQGEPAGFALYFFNFSTFVGRPGLYLEDLFVRPAQRSHGIGRALLR
                     ALARIAKERGCGRMEWAVLDWNEPALRFYQSLDARQMKEWIIHRLTPVEIARLAAES"
     gene            complement(994..1926)
                     /locus_tag="VIV63_03775"
     CDS             complement(994..1926)
                     /locus_tag="VIV63_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123745.1"
                     /translation="MRVRALIFTVLLASACLPASAAGPNGAQQRGAEAFLAALAGGNV
                     MAVAQELHPDEVEKLRTRLLALFRAEASRSDNTYRSRLFGPGRSLAELESMTPVRFYA
                     ALSDRVRFRAREYDKVDWLGVVPDGKVVYVIGKGVPPKDRGAVKVLVTVALAQYGAQW
                     RAIIPSEVEAQLDDLIEGRVAAAPPPSGGAPGRSLVEERPAPLSPGISELLARAEASL
                     AAGHCEEYYSQFMSPNVTRAMSKSAKKTLITACSNSESTRETMITTLRVVQELTPKYD
                     LGGARAIYDVSGQGLPYDRFVLERDKDMRWYIAE"
     gene            2152..>2573
                     /locus_tag="VIV63_03780"
     CDS             2152..>2573
                     /locus_tag="VIV63_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123746.1"
                     /translation="MLPQSEFESPMLASAAAPVAIAAITQDRALIGLLRSVIDPSNDL
                     ILVTSEAELTPHLNGRRVSVALLDSMFIEGDLAAMAERMRETWPDLVLVVVGTAEEQS
                     KVAGQITSGVVYRFLHRPVSAPRVRLFVDAALRRHEVE"
BASE COUNT          430 a          867 c          825 g          451 t
ORIGIN      
        1 agcgcccgcg gccgcggccg caacgccgcc cggcatggtc acggttccgg ccacctcgac
       61 ttacatcccc gcgggcgccg cgccggcgat cagcaccgac ccgggagtcg tgacgccaag
      121 ccacacgccg ccactcgccg aggaacccgt gctgtccggc caggaacctt ccgtgccgcc
      181 ggactacgac gcgcgcgact accggccggc gatcactccg gcacaggcga acgcggtcgc
      241 ggcggcacgg cgtgaaatgt cggcgccgtc acgcgacgaa gtggtggcgc agggaacaca
      301 attgcccgac ttgcggctcg acctgcacgt gtacgacccc gctcccgcga agcgattcgt
      361 gttcgtcaac atgcgcaagc tccgcgaagg cgactccacg cccgagggcg tgcggctcga
      421 cacgatcacg cagacgggcg cgaagttctc gttccgcggc accgacttca cgatcgaggc
      481 gaactgagtc gaggctcgcc gggtcagctc tcggccgcga ggcgcgcgat ctcgaccggc
      541 gtgaggcggt ggatgatcca ctccttcatc tgccgcgcgt cgagtgactg gtagaagcgc
      601 agcgccggct cgttccagtc gagcaccgcc cattccatgc gcccgcagcc gcgttctttt
      661 gcgatgcgcg ccaacgcccg caacagcgcg cgcccgatgc cgtgactgcg ttgcgcgggg
      721 cgcacgaaca ggtcttcgag atacaggccc ggacggccga cgaacgtcga aaaattgaag
      781 aaatacagcg cgaaacccgc gggttctccc tgccattcgg cgatcagcac gtgaatcacc
      841 ggattcggtg actcgagctc gcgcaggaaa tcctgaggcg tcgccttcgc ctcgtcgagc
      901 agcttttcgt agtccgcgag ctcgcgaacc aggttcgcga tgaccggcgc gtccgcgcgc
      961 gtcgcgggtc ggatgttcag catgggtttc tctttactcc gcgatgtacc agcgcatgtc
     1021 cttgtcgcgt tccagcacga agcgatcgta gggcaggccc tggccgctca cgtcgtagat
     1081 cgcgcgtgcc ccacccagat cgtatttcgg cgtgagctcc tggacgacgc gcagcgtggt
     1141 gatcatggtc tcgcgcgtgc tctcgctgtt ggaacaggcg gtgatcagcg tcttcttcgc
     1201 ggatttcgac atcgcccggg tgacattggg gctcatgaac tggctgtagt actcctcgca
     1261 atggccggcc gcgaggctgg cttcggcacg tgccagcaat tcgctgatgc ccggcgaaag
     1321 cggcgcgggt ctttcctcga ccagcgatct gcccggcgcg cctcccgacg gcggcggcgc
     1381 ggcggcaacc cggccttcga tgagatcgtc gagctgcgct tcgacttcac tggggatgat
     1441 ggcgcgccac tgcgcgccgt actgggcgag cgcgacggtg accagcacct tcaccgcacc
     1501 gcgatccttg ggcggcacgc ccttgccgat gacgtaaacc accttgccgt ccggaaccac
     1561 gccgagccaa tctactttgt cgtattcgcg cgcgcggaaa cgcacgcggt cggacagcgc
     1621 ggcgtagaac cgcaccgggg tcatgctctc gagctccgcg agtgaccttc cgggtccaaa
     1681 caaccgcgag cgataggtgt tgtcggaccg cgaggcttcg gcgcgaaaca gcgcgagcag
     1741 gcgcgtccgg agcttttcca cctcgtcggg atgcagttcc tgggcgaccg ccatgacgtt
     1801 gccgccagcc agtgcggcga gaaaagcttc cgcgccacgt tgttgggcgc cgttgggccc
     1861 ggcggcggat gccgggagac aggccgaagc caacaacacc gtgaaaatca gggctctaac
     1921 gcgcatttgg cactccaatc gaatacggcg gcggcgatca taaccgggcg gcgaaaggcc
     1981 gccccaactg tgattgtggt ctcgttacca gacattggtg gcgggacgca gttcacggat
     2041 tacccgcgcg cgaggccttg gtgcgacctt ccccgataga attacatatc ttgttaaagt
     2101 gctttcgcac cctttgtttt cgctgctttt cgcgggtttg ccaccaccgt catgttgccg
     2161 cagtcggaat tcgaatcgcc catgctcgcc agtgccgcgg cgccggtggc gattgccgcg
     2221 attacgcagg accgcgcgct gatcgggctc ctgcgcagcg tcatcgatcc ttccaacgat
     2281 ctcatcctcg tcacctctga agcggagctc acgccgcatc tgaacggccg ccgcgtgtcc
     2341 gtggcgctgc tcgacagcat gttcatcgaa ggcgacctcg ccgcgatggc ggagcgcatg
     2401 cgtgaaacat ggccggatct cgtgctcgtg gtggtcggca ccgcggagga acagtcgaag
     2461 gtcgccggac aaatcacctc cggcgtggtg tatcgattcc tgcatcgccc ggtctctgcg
     2521 ccgcgcgtgc ggttgttcgt cgatgctgcc ctgcgccgcc atgaagtgga aaa
//
LOCUS       DATDEU010000151         4378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2068742, whole genome shotgun sequence.
ACCESSION   DATDEU010000151 DATDEU010000000
VERSION     DATDEU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068742"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2279
                     /locus_tag="VIV63_03785"
     CDS             <1..2279
                     /locus_tag="VIV63_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123747.1"
                     /translation="TELAEALSTAAPSLNFPRPSITDGTDHIRPATLRGLAPDQTLVL
                     VNSKRRHQSALVNVNGSVGRGSAAVDLNAIPLAALETVEVLRDGASAQYGSDAIAGVI
                     NLRLRDAREGGSATLTYGEYNTDVQTERGQRSEDDGPTLTASGWVGLPLGQEGFLTLS
                     GEYRDRDPTSRGDFDNRVPGSPVTSRYGDANVEDITAYVNAGVPLEGDWNIYGWAGYQ
                     QREGDSAAFPRIFNDVRNVPGIYPNGFLPLITTDIDDLALGWGVRGKLGSWDADVSLV
                     YGSNEVVYGVENSLNTSYGIDSQTEFDAGAMKYDQIVFNAGIVRTFEVGNLPEGINVA
                     FGIEARQENYQIEAGEPTSYNRGPIVGVAAGAQGFPGFQPSNEVDEDREAYGIYADVE
                     ARLTEKFLASVAVRAEDYSDFGSAVTGKLAGRYDFTDSFALRGAVSTGFRAPGLQQEF
                     FTSTATNFINGEPFEVGTFPATSDVAITLGAQPLDAEESVNYSLGTVFRFGGFEATID
                     AYRIDIDDRIVLSENLQSPQVAALIAPFGANIARFFINGVETKTEGIDLVTRYLLRTE
                     GLGKFEFVAAGNWNDTEVEEVPSTNVIDNICVGQPAPCTPPVLFARVNTLAFEEGTPD
                     SKVSLGVDWVMPLGGTNFGVNLKGTRYGDVIEPGVPSTAEINAGVPDSRDLHIQADWV
                     VDLTLTAKLLEDKLSFSLGADNLFDQYPDRIPNARVLPTPPGGTVNLNATNALAYSRY
                     SPYGFNGRFVFARMAYNW"
     gene            2395..2790
                     /locus_tag="VIV63_03790"
     CDS             2395..2790
                     /locus_tag="VIV63_03790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6123748.1"
                     /translation="MEGLRVCPYRFSVATTYRLPTLTSRREFFRLPYPVTTGATLAVE
                     GANYKVGEVSERGLRIVSDVDKFPLESAIHGTLILTMGLRCTVAGKVLRVEDGAVVVK
                     LDRGPTTYDVIREQRHLTKTFPDWKPLPA"
     gene            2922..>4378
                     /gene="typA"
                     /locus_tag="VIV63_03795"
     CDS             2922..>4378
                     /gene="typA"
                     /locus_tag="VIV63_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY6123749.1"
                     /translation="MSSMANRPIRNIAIIAHVDHGKTTLVDCLLRQSGTFAAHEKVTE
                     RVMDSNDIEKERGITILAKNCAIEYGGTRINIVDTPGHADFGGEVERVLSMVDTVLLV
                     VDAVEGPMPQTRFVTRKALALGLNAIVVVNKIDRPGARPDWVVDQTFELFDKLGATDR
                     QLDFPVIYASALEGWASTDHTKREPDMRAMYEAILHHVPAPKSEPEAPLQFQISTLDY
                     NSYVGRIGIGRIRRGTIKAGQTIQLWNGTEDHGTAKVQQVLTFKGLARESVDEAVAGD
                     IVAVTGIEDVHIGYTINEVDKPDALPPIAVDEPTLVMNFQVNTSPLAGKEGKFVTSRQ
                     IRERLMRELESNVALRVDMTADADVFRVSGRGELHLTILIENMRREGYELAVGKPRVL
                     TKTIDGVVHEPFEALTVDIDEGHQGGVMEALGRRKAELKDMANDGRGRVRIEYRVSAR
                     GLIGFQSEFMNATRGTGLQSHIFDGYAPLAGDIPDR"
BASE COUNT          827 a         1480 c         1337 g          734 t
ORIGIN      
        1 gcaccgaact cgcggaggcc ctatccaccg ccgcgccgtc gctgaacttc ccgcgcccgt
       61 ccatcaccga tggcacggac cacattcgcc ccgccacgct gcgcggtctc gcacccgatc
      121 agacgctggt gctcgtgaac agcaagcgcc ggcatcagtc cgcgctggtc aacgtgaacg
      181 gctcggtggg ccgcggctct gccgcggtcg atctcaacgc catcccgctg gcggcgctcg
      241 agaccgtcga agtgctgcgc gacggcgcgt cggcgcaata cggttcggat gccattgccg
      301 gtgtcatcaa cctgcgcctg cgcgatgccc gcgagggcgg cagcgccacg ctgacctatg
      361 gtgaatacaa caccgatgtc cagaccgagc gcggccagcg cagcgaagac gatggtccca
      421 ctctcacggc gtcgggttgg gtaggtttac cgctcggcca ggaaggcttc ctcacgttgt
      481 caggcgaata tcgcgatcgc gatcccacga gccgcggcga tttcgacaat cgcgtgccgg
      541 gctcacccgt gacctcgcgt tatggcgatg cgaacgtcga agacatcacc gcctatgtga
      601 acgccggcgt gccgctcgaa ggcgactgga acatctacgg ctgggccgga taccagcaac
      661 gcgaaggcga ctccgccgcg ttcccgcgca tcttcaacga cgtgcgcaac gtgccgggca
      721 tctacccgaa cggcttcctg ccgctcatca ccaccgacat cgacgacctg gccctgggct
      781 ggggcgtgcg cggcaagctg ggtagttggg atgccgacgt gagcctggtc tacggctcca
      841 acgaagtggt ctacggcgtc gagaacagtc tcaacacgtc gtacggaatc gactcgcaga
      901 ccgagttcga cgccggcgcc atgaagtacg accagatcgt tttcaacgcc ggcatcgtgc
      961 gcactttcga agtgggcaac ctgcccgagg gcatcaacgt cgcgttcggc atcgaagcgc
     1021 gtcaggagaa ctaccagatc gaagccggcg aacccacctc ttacaatcgc ggccccatcg
     1081 tcggcgtcgc ggcgggcgcg cagggcttcc ccggattcca gcccagcaac gaagtggacg
     1141 aagaccgcga agcgtatggc atctacgcgg atgtcgaagc gagactcacg gagaaattcc
     1201 tggcctcggt cgccgtgcgc gccgaagact attcggactt cggctccgcc gtcaccggca
     1261 aactggccgg acgttacgac ttcaccgatt cattcgcgtt gcgcggcgct gtctccacgg
     1321 gcttccgcgc cccgggactg cagcaggagt tcttcacctc cacggccacc aacttcatca
     1381 atggcgaacc gttcgaagtc gggaccttcc cggcaacttc ggatgtggcg atcacgctcg
     1441 gcgcacagcc gctcgatgcc gaagagtcgg tcaactattc gttgggcacg gtgttccgct
     1501 tcggcggctt cgaggcgacc atcgacgcgt atcgcatcga catcgacgat cgcatcgtgt
     1561 tgtcggaaaa cctgcagtcg ccgcaagtcg cggcgctcat cgcacctttc ggcgcgaaca
     1621 tcgcgcggtt cttcatcaac ggcgtcgaga cgaaaacgga aggcatcgac ctggtgacgc
     1681 gttacctgtt gcgcaccgaa ggtctcggca agttcgaatt cgtcgcagcc ggcaactgga
     1741 acgatacgga agtggaagaa gtgccctcca ccaatgtcat cgacaacatc tgcgtgggcc
     1801 agcccgcgcc gtgcacaccg cccgtgctgt tcgcgcgggt caacacgctg gctttcgagg
     1861 aaggcacgcc cgacagcaag gtcagcctgg gagtcgactg ggtcatgccg ctcggcggca
     1921 cgaacttcgg cgtgaatctc aagggcacgc gctacggcga cgtcatcgaa ccaggcgtgc
     1981 cgtcgacggc ggaaatcaac gccggcgttc ccgattcacg cgacctgcac atccaggcgg
     2041 actgggtcgt cgacctcacg ctgaccgcca aactactcga ggacaagctg tcgttcagcc
     2101 tgggcgccga caacctgttc gaccagtatc cggaccgcat cccgaacgcg cgcgtcctgc
     2161 cgacgccgcc cgggggcacg gtgaacctca atgccacgaa tgcgctggcc tattcgcggt
     2221 attcgccgta cggcttcaac gggcgcttcg tgttcgcccg catggcctac aactggtagt
     2281 gcggcgtata taaaggtgtc gaaaaggggc tgcctggcgt caggcagccc cgcggacggt
     2341 tcaattattt gacggagttc gcatttcggc tttcgcggga tgcgaacttt cccgatggaa
     2401 ggcctcaggg tttgcccgta tcgtttcagc gtggccacca cttatcgcct acccactctg
     2461 acgagccggc gcgagttctt tcgcctgccg tacccggtta ccacgggtgc gacgctggcg
     2521 gtggaaggcg ctaactacaa ggtcggcgaa gtgtccgaac gcggcctgcg catcgtctcg
     2581 gacgtggaca agttcccgct cgaatcggcc attcacggca cgctcatcct caccatgggc
     2641 ttacgttgca cagtggccgg caaggtcctg cgcgtcgaag acggcgccgt ggtggtgaaa
     2701 ctcgaccgcg gccccacgac ctacgacgtc atccgcgagc agcggcacct gaccaagacc
     2761 ttcccggact ggaagccgct gccggcctga gtccacggaa ttgcccggag ttcgggggca
     2821 gttcgcgtct ttccgcagcc ttctttggcg tttaaccgcc catgtggtac cgtgcgcgcc
     2881 ccttggcccc tgcctgcgcg ccccttccct cgcgtaagcc catgagttct atggcaaatc
     2941 gtccgattcg aaacatcgca atcatcgcgc acgtcgacca tggcaagacc acgctggtcg
     3001 actgcctgct gcgtcaatcc ggcaccttcg ccgcgcacga gaaagtgacc gaacgcgtca
     3061 tggactcgaa cgacatcgaa aaggaacgtg gcatcacgat cctcgcgaag aactgcgcca
     3121 tcgaatacgg cggcaccaga atcaacatcg tggacacgcc cgggcacgcc gacttcggcg
     3181 gtgaagtgga acgcgtgctg tcgatggtcg acaccgtgtt gctggtcgtc gacgccgtcg
     3241 aaggcccgat gccgcagacg cgtttcgtca cgcgcaaggc gttggcgctc ggcctcaacg
     3301 ccattgtcgt cgtgaacaag atcgaccgtc cgggcgcgcg ccccgactgg gtcgtggacc
     3361 agaccttcga actgttcgac aaactcggcg ccacggatcg tcagctcgac ttcccggtga
     3421 tctacgcctc ggccctcgaa ggctgggcga gcacggatca caccaagcgc gagcccgaca
     3481 tgcgcgccat gtacgaagcc atcctgcatc acgtgccggc gcccaagagc gagcccgagg
     3541 ctccgctgca gttccagatt tccacgctcg actacaactc gtacgtcgga cgcatcggca
     3601 tcggccgcat ccgccgcggc acgatcaagg ccggccagac gatccagctc tggaatggca
     3661 ccgaagatca cggcaccgcg aaggttcagc aggtgctcac gttcaagggc ctggcgcgtg
     3721 agtcggttga cgaagcggtc gccggcgaca tcgttgccgt cacgggcatc gaggacgtcc
     3781 acatcggcta cacgatcaac gaggtcgaca agcccgacgc gttgccgccg atcgcggtcg
     3841 acgagcccac gctcgtcatg aacttccagg tgaacacctc gccgctcgcc ggcaaggaag
     3901 gcaagttcgt caccagccgg cagatccgcg aacgcctcat gcgcgagctc gaaagcaacg
     3961 tcgcgctgcg cgtcgacatg accgccgacg ccgacgtgtt ccgcgtctcg ggccgcggcg
     4021 aactgcatct caccatcctc atcgagaaca tgcgccgcga aggttacgag ctcgccgtgg
     4081 gcaaaccccg cgtgctcacc aagaccatcg acggcgtcgt gcacgagccg ttcgaagcgt
     4141 tgaccgtcga catcgacgaa ggccaccagg gtggtgtcat ggaagcgctc ggccgccgca
     4201 aggcggagct caaggacatg gcgaacgacg gccgtggccg cgtgcgcatc gagtaccgcg
     4261 tgtccgcgcg cggactcatc ggcttccaga gcgagttcat gaacgccacg cgcggcaccg
     4321 gtctgcaaag tcatattttc gacggctacg cgccgctcgc gggtgacatc ccggaccg
//
LOCUS       DATDEU010000152         3225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1922456, whole genome shotgun sequence.
ACCESSION   DATDEU010000152 DATDEU010000000
VERSION     DATDEU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922456"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2358
                     /locus_tag="VIV63_03800"
     CDS             <1..2358
                     /locus_tag="VIV63_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123750.1"
                     /translation="GLFDLLLASKAPAPAAAPPPAPYESVLAEEAPSADAAAAATDES
                     ERITVTGNRSTLNSIQAVSRYAAGTVLQAGPGIPAWRYNSYNYFWTGPVETADTVRFI
                     YVGPVVMFFWRIIGTLAVTVLFLWLARLSFGGTWTLPSMPFARRAAATALLLVVAFAG
                     VSPARAQSQPNPAPSTPGTELLSELKSRLTAAPRCAPRCAEITAANVSVNGERLDVVL
                     QVSALANIAVAMPHASDRWQLDEVSVDSRAAVAIARNSDTSLWVPLTPGAHSVRLSGR
                     LASAESIQLVFPQPPRVIDVRASGWTVNGVNESRLVAGSLELARERTAQRAGDTLEAG
                     SEFPGFVRVERVFNLDLDWTIDTEVVRIAPQRAALSVEVPLVKGESVLTPGVEVRNGE
                     TALVGLGTGESSTRWHSGLARAETLELSVPADASRSEVWSFEVNPQWNVSFEGFAPVL
                     PQNVDAAVWSFRYMPRPGEKLTLKVTRPKPVSGTTLAIDAVTQTIEVGRRSSNTTLRF
                     SSRSTQGGRHVIKLPPDARVTSVLFDQQPQQLRPEKGELPLSLTPGSHEIEVRWERSE
                     DVGVRTHPPAVDLQSQASNISLGITLPESRWTLFAWGPGVGPAVLYWGELVVFVGIAW
                     LLGRWSRSPLRFSEWLLLGLGLSTLSWWVFTFTGLWLIAMKWREGWVPHEALSRTRFN
                     AVQALLALLTLFVITTLVFSGIRNGLLAAPDMGIISTVYAGGALSWFQDQTKGVIETP
                     TVISAPMWVYRALFFAWASWMAFALVRWLRWAFNAWKTNGLWRTE"
     gene            complement(2481..2861)
                     /locus_tag="VIV63_03805"
     CDS             complement(2481..2861)
                     /locus_tag="VIV63_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123751.1"
                     /translation="MKKQVASNDSDPGPRLHTVNQYLEKNFPDFFAEARFHVGNDDYF
                     LYARFGKYLASSIEHRRIKSEKISRGFTVLNKLARKAEHDPKVRHILVSGPLEEIVDE
                     PKARELARKRLSPVAQGYLEGLVE"
     gene            2982..>3225
                     /locus_tag="VIV63_03810"
     CDS             2982..>3225
                     /locus_tag="VIV63_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123752.1"
                     /translation="MKFLTLLVTASLLAACAAEPSDEERVRSVIESVEEAAEARDTSD
                     VLEHVADNYADARGMDKTQLQGFLRGYFLAHPKLELL"
BASE COUNT          521 a         1030 c         1078 g          596 t
ORIGIN      
        1 ggcctgttcg atctgctcct ggcttcaaag gcacccgcgc ctgcggccgc gccaccgccc
       61 gcgccgtatg agagcgtttt ggccgaagag gcgccgtctg ccgacgccgc ggccgcagcg
      121 acggatgaat ccgaacgcat cacggtcacc ggaaaccgaa gcacgctgaa ctccatccag
      181 gcggtgtcgc gttatgccgc gggcaccgtg ttgcaggcag ggcctggcat ccccgcgtgg
      241 cgatacaact cctataacta cttctggacc ggccccgtcg agaccgccga caccgtgcgc
      301 ttcatttatg tcgggccggt cgtcatgttc ttctggcgca tcatcggcac gcttgcggtg
      361 acagtgctgt tcctgtggct ggcgcgactg agtttcggcg ggacctggac tctgccctcg
      421 atgccgttcg ctcgccgcgc ggcggcgacc gcgctgttgc tggtcgtggc attcgcggga
      481 gtttcgccgg cacgcgccca gtcccagccc aacccggcgc cgtcgacccc cggaaccgaa
      541 ctgctgtccg agctcaagtc tcgactcacg gccgctccgc gttgcgcccc gcgttgcgcc
      601 gagatcaccg ccgccaatgt cagcgtcaac ggcgagcggc tcgacgtcgt gctgcaggtc
      661 agcgcgctcg ccaacatcgc cgtggcgatg ccgcatgcca gtgatcgatg gcagctcgac
      721 gaggtgagcg tggactcgcg cgccgcggtc gccatcgcgc gcaacagcga cacgtcgttg
      781 tgggtgccgt tgacgcccgg agcgcacagc gtgcgcctgt cggggcggct tgcgtccgca
      841 gaatcgattc agctcgtgtt cccgcagccg ccgcgcgtca tcgacgtgcg cgcctcggga
      901 tggacggtga atggcgtgaa cgagagccgg ctcgtcgcgg gctcgctgga gctggcgcgc
      961 gaacgcaccg cgcagcgcgc cggcgacaca ctcgaagccg gcagcgaatt cccgggcttc
     1021 gtgcgcgtgg agcgcgtctt caatctcgat ctcgactgga cgatcgatac cgaagtcgtg
     1081 cgcatcgctc cgcagcgcgc ggcgttgtcc gtggaagtcc cgctcgtgaa gggcgaatcg
     1141 gtgctcacgc ccggtgtgga agtgcgcaac ggcgagacgg cgctggtggg cctcggtacc
     1201 ggcgaatcca gcacgcgctg gcattcggga ctggcgcgcg ccgaaacgct ggagctgtcg
     1261 gtgccggcgg atgcgtcgcg cagcgaggtc tggagtttcg aggtcaatcc gcaatggaac
     1321 gtgtccttcg agggcttcgc gccggtgctg ccacaaaacg tggacgctgc cgtctggtct
     1381 ttccgttaca tgccgcgccc gggcgagaag ctcacgctca aggtgacacg acccaagccg
     1441 gtgagcggca ccacgctcgc catcgatgcg gtgacgcaaa ccatcgaggt gggacgtcgc
     1501 tcgtcgaaca ccacgctgag gttcagctca cgcagcacgc agggtggccg tcacgtcatc
     1561 aaactcccgc cggacgcgcg cgtgacctcc gtgctcttcg accagcagcc gcagcagctt
     1621 cgtccggaga agggcgagtt gccgctgtcg ctcacgccgg gtagtcatga gatcgaggtg
     1681 aggtgggagc gctccgagga cgtcggcgtc agaacccatc cgccggccgt cgacctgcaa
     1741 tcgcaggcga gcaacatctc gctcggcatc acgctgcccg agtcgcgctg gacgttgttc
     1801 gcgtgggggc cgggcgtcgg acccgcggtg ttgtactggg gcgagctcgt ggtgttcgtc
     1861 ggcattgcct ggctgctcgg gcgctggtca cgttcgccgc tgcgattctc cgagtggctg
     1921 ctgctgggac tggggctctc gacgctgtcc tggtgggtgt tcacgttcac cggcctctgg
     1981 ctgatcgcga tgaagtggcg cgaagggtgg gtgccgcacg aggcgctgtc gcgaacgcgc
     2041 ttcaatgccg ttcaggcgtt gctcgcgctg ctcacgttgt tcgtgatcac cacgctggtg
     2101 ttctccggca ttcgcaacgg attgctggcc gcgccggaca tgggaatcat ctccacggtg
     2161 tatgccggcg gcgcgctgtc gtggttccag gatcagacga agggagtcat cgagacgccc
     2221 accgtgatct ccgcgccgat gtgggtttac cgcgcgttgt tcttcgcgtg ggcgagctgg
     2281 atggcattcg cgctggtgcg ctggctgcgc tgggcgttca acgcctggaa gacgaacggc
     2341 ctgtggcgca cggagtagtg gaggcgaaat ggggacactc cccatttccg ggaaacgggg
     2401 ccgcacctgg gtgcggcctc atctgctgtc gttaccaacg aggtccgtcc ccatttccta
     2461 ggaaatgggg agtgtcccca ttattcgacg aggccttcga ggtagccctg cgcgaccggc
     2521 gacaggcgct tgcgcgcaag ttcgcgggcc ttgggctcat cgacgatctc ttccagcggg
     2581 ccggacacga gaatgtgccg caccttggga tcatgctccg ccttgcgggc gagcttgttc
     2641 agcacggtaa aaccgcggga gatcttttcg ctcttgatgc gccggtgctc gatggagctg
     2701 gcgaggtatt taccgaagcg ggcgtacaga aagtaatcgt cgtttccaac gtggaaacgg
     2761 gcttcggcaa agaagtcggg gaaattcttt tctagatact ggttaacagt atgaagacgg
     2821 ggtccagggt cgctgtcgtt cgacgcaact tgtttcttca tttaagtggt ccacctccaa
     2881 ctgggtccgg ggcaccattt ctggacgtca cgaccggttc ccaaccccgc aacgcgcgcg
     2941 aacgataggg ctaagcgact tcaactgcaa taggacgaaa catgaaattt ctcacactcc
     3001 tcgtcaccgc cagtctcctc gccgcgtgtg ccgcggagcc gagcgacgaa gaacgcgttc
     3061 gctccgtcat cgagagcgtc gaagaagcgg ccgaggcgcg cgatacgagc gacgtactcg
     3121 agcacgtcgc cgacaactac gccgacgcgc gcggcatgga caaaacgcag ctccagggat
     3181 tcctgcgcgg ctatttcctg gcgcatccga agctcgaact gctgg
//
LOCUS       DATDEU010000153         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1048244, whole genome shotgun sequence.
ACCESSION   DATDEU010000153 DATDEU010000000
VERSION     DATDEU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048244"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /locus_tag="VIV63_03815"
     CDS             complement(<1..1740)
                     /locus_tag="VIV63_03815"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY6123753.1"
                     /translation="MATAPQSLLSLPEISPAVADWVESVRKLTTPDKVQWCDGSPAEL
                     ERLKQTLEKSGELKKLNQTTFPGCHIAYSHPSDVARVEHLTFICTKNREDAGPNNNWM
                     APDEARAKMNALFSGCMKGRTLYVVPYCMGPIDSPYSRCGVEITDSAYVVINMSIMTR
                     MGRLALERIAREGTFVKGLHSIGELDPNRRFIMHFPEDLSIQSFGSGYGGNALLGKKC
                     HALRIASWQARDEGWLAEHMLIVGIENPQGETHYVAAAFPSACGKTNLAMLIPPDSMP
                     GWKIWTVGDDIAWLHPGPDGRLYAINPESGYFGVVPGTNRATNRNAFDMIHRDTLFTN
                     VALTADNEPWWEGRKLNTPVLDWQGKPFDPASGGVSAHPNSRFTVSARNNPIYSPRSE
                     DAMGVPISAIVFGGRRREVAPLVYEARNWQHGVVVGASVASETTAAATGAVGVVRRDP
                     MAMKPFAGYNFGDYWAHWLDMGKRLKSPPRLFHVNWFRRDASGKFLWPGFGDNLRVME
                     WILKRCAGKVGAEDSPIGYLPKPADLNLKGADISDATMQELLAVTPAAWRKETAEMRE
                     YLKEFGDRAPAEMS"
     gene            complement(1897..2256)
                     /locus_tag="VIV63_03820"
     CDS             complement(1897..2256)
                     /locus_tag="VIV63_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123754.1"
                     /translation="MNFQERMLEATAQLRARTAALATVAVTTARTQAQVAAKRVGGLK
                     GSLTALKTVGRELNKVARLHGTRFVKENSAIAVGAGKDLSALARTTYAALAKRPAAAR
                     KTRKAPAARKRAARKAA"
     gene            complement(2333..>3145)
                     /gene="phaZ"
                     /locus_tag="VIV63_03825"
     CDS             complement(2333..>3145)
                     /gene="phaZ"
                     /locus_tag="VIV63_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HEY6123755.1"
                     /translation="PKVEFVEVDGVNLRVASQTGNPGLPPLLLFNGIGANLELCFPFM
                     EAMPEREIVIFDVPGVGRSEMSWRPRRFSGLARLANKLLDRQGYREVDVIGVSWGGAL
                     AQQFARQYPQRCRRLILAATSPGVVMVPGRPSALSKMVTPRRYLSPSYMQKAAGDIYG
                     GEARRNPGLMSAHTSRIIPPQFMGYVYQLFAGMGWTSIHWLHRIRQPTLVMAGSDDPL
                     VPPVNSRIISFLIPNNRLHIVPGGGHLFMLYSIDKVVPVIRAFLESEAPLTV"
BASE COUNT          571 a          946 c         1049 g          579 t
ORIGIN      
        1 ggacatctcc gccggggccc gatcaccaaa ctccttcagg tattcacgca tctcggcggt
       61 ttccttgcgc caggccgcgg gcgtcacggc cagcagttcc tgcatcgtgg cgtcgctgat
      121 gtccgcgccc ttcaggttga ggtcggcggg cttcggtaga tagccgatcg gcgagtcttc
      181 cgcgccgacc ttgccggcgc agcgtttcag gatccattcc atgacgcgca ggttgtcgcc
      241 gaatccgggc cagaggaact tgccggaagc gtcgcgacgg aaccagttga cgtggaacag
      301 gcgaggcggg ctcttcaacc gcttgcccat gtccagccag tgagcccagt agtcgccgaa
      361 gttgtagccg gcgaatggct tcatcgccat cggatcacgg cgcacgacgc cgaccgctcc
      421 ggtggctgcg gccgtggtct cggaggcgac cgatgcgcca acgacgacgc cgtgttgcca
      481 gttgcgggct tcgtaaacca gcggcgcgac ttcccgtcgc cggccgccga acacgatggc
      541 cgagatggga acacccatcg cgtcttcgct gcgcggtgag tagataggat tgttccgggc
      601 ggacacggtg aatcgggagt tcggatgcgc cgagacgccg ccgctggccg gatcgaaagg
      661 tttgccctgc cagtcgagta ccggagtgtt gagcttacgc ccctcccacc agggttcgtt
      721 gtccgcggtc agcgcgacgt tggtgaacaa cgtgtcgcgg tgaatcatgt cgaacgcgtt
      781 acgattggtg gcgcgattgg tgccgggtac cacgccgaag taacccgatt cggggttgat
      841 ggcatacaac cgcccatcgg ggccgggatg cagccaggcg atgtcatcgc cgaccgtcca
      901 gatcttccag ccgggcatgg agtcgggcgg aataagcatc gcgagattgg tcttgccgca
      961 ggccgacgga aacgccgcgg cgacgtaatg cgtttcgcct tgcggattct cgatgccgac
     1021 gatcagcatg tgttccgcca gccagccttc atcgcgcgcc tgccagctgg cgatgcgcaa
     1081 tgcgtggcat ttcttgccga gcagcgcatt gccgccgtag ccggaaccga agctctggat
     1141 cgaaagatct tccggaaagt gcatgatgaa gcggcgattc ggatcgagct cgccgatgga
     1201 gtgcaggccc ttgacgaatg ttccctcacg cgcgatccgc tcgagggcga ggcgccccat
     1261 ccgcgtcatg atggacatgt tgatgacgac gtaggcgctg tcggtgatct cgacgccgca
     1321 gcgcgagtac ggagagtcga tgggacccat gcagtagggg acgacgtaca acgtccgacc
     1381 cttcatgcag ccgctgaaca gcgcgttcat tttcgcgcgc gcttcgtccg gagccatcca
     1441 gttgttgttg gggccggcgt cttcgcggtt cttcgtgcag atgaaagtca gatgctcgac
     1501 gcgcgccacg tccgaggggt gggagtaggc gatgtggcag cccgggaagg tagtttggtt
     1561 gagcttcttg agctcgcccg acttctcgag agtctgcttc aggcgttcca gttctgccgg
     1621 agagccgtca caccactgca ccttgtcagg tgtcgtgagc tttcgaacgg actcgaccca
     1681 gtcggcgacc gcgggggaaa tttccgggag agagagaagc gactggggcg ctgtggccat
     1741 cactgtacct tcgaatgtct gtctgtcctg acgagacaaa ggagtatagc ggcgcgaaaa
     1801 acgtcacggc ggtcagaaac gaatcgctcg atcacagaat aaggacgcgc cccgcgaggg
     1861 gcgcgtccaa tgtcacttgc ccggcctgta gcggaatcag gccgccttgc gggccgctcg
     1921 cttgcgcgcg gcaggcgcct ttcgcgtttt gcgcgcggct gcggggcgct tggccagcgc
     1981 ggcgtaggtc gtgcgggcga gggccgagag gtccttgcca gcgcccacgg cgatggccga
     2041 gttctctttg acgaaccgcg tgccatgcag gcgggcgacc ttgttcaact cgcggcccac
     2101 ggttttgagc gcggtcagtg agcctttcag gcccccaacc cgcttggcgg caacctgagc
     2161 ctgcgtgcgg gcggtcgtga cagcgacagt cgccaaggcg gccgtacggg cgcgcaattg
     2221 cgcggtggct tcgagcattc tttcctggaa attcatggtt tctcctatgc tgcgtcgcag
     2281 caatgatgcg gtgcatcata ctcacgagaa gatcggagtc aaggaccaga agctaaaccg
     2341 tgagcggcgc ctctgattcg aggaacgcgc ggatgaccgg gaccaccttg tctattgaat
     2401 agagcatgaa gaggtgaccg ccccccggga ctatgtgcag ccggttattg gggatgagga
     2461 aagagatgat gcgcgagttc actgggggca ccagagggtc gtcagacccc gccatgacga
     2521 gtgtgggttg gcgaattcgg tgcagccaat ggatgctggt ccagcccatg cccgcgaaca
     2581 gctggtacac ataacccatg aactgcggcg gaatgatgcg cgacgtatgc gcggacatga
     2641 ggcccggatt gcgccgggct tcgccgccgt agatgtcacc ggcggccttc tgcatataag
     2701 aaggtgagag gtagcgtcgc ggcgtgacca tcttcgacag cgccgacggg cgtcctggca
     2761 ccatgacgac gcccggagat gtcgccgcga gaatcagtcg ccggcaccgt tgcggatatt
     2821 gccgcgcgaa ctgctgggcg agtgcaccac cccaggaaac gccgatcaca tcgacttcgc
     2881 ggtagccctg ccggtcgagc agcttgttgg cgagccgcgc gagtccggaa aagcgccgtg
     2941 gccgccagct catctccgag cggccgacac cgggcacgtc gaagatcacg atttcccttt
     3001 ccggcatggc ctccatgaaa gggaagcaca gttccaggtt cgcaccgatg ccgttgaaca
     3061 gcagcaatgg cggcagcccc gggttgccgg tctggctcgc gacgcgaaga ttgacgccat
     3121 cgacctcgac gaactcgact ttggg
//
LOCUS       DATDEU010000154         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_901677, whole genome shotgun sequence.
ACCESSION   DATDEU010000154 DATDEU010000000
VERSION     DATDEU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901677"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VIV63_03830"
     CDS             complement(<1..624)
                     /locus_tag="VIV63_03830"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY6123756.1"
                     /translation="MDAIAALLSDPQVWIAFFTLTALELVLGIDNIIFISILVDKLER
                     VQREKARRIGLFLAMFMRIGLLMVLSWIVGLTAPLFTITGTEISGRDLILIAGGVFLV
                     WKSTMEIHQLTEGEEGHASKSVKASFGAIILQIIIIDMVFSLDSIITAVGMVDEVSVM
                     IAAVIASVGLMMLFARAIGDFVSAHPSIKMLALSFLLLVGVMLIAEGF"
     gene            674..>2486
                     /locus_tag="VIV63_03835"
     CDS             674..>2486
                     /locus_tag="VIV63_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HEY6123757.1"
                     /translation="MLKLIIRLFAAFVFLSLAMPATARLLSGEVHVWEIQEITLAATR
                     DYANPYADIECWVELEGPDFRRRVYGFWDGGRTFKVRIVATQPGDWTWRAGSNRDDPG
                     LAGSGKFRAVAWSEAERAQNPNRRGFVRASENGHALRYADGTPFFLVGDTWLAASTWR
                     LPFKGVRAADDSVPAEGISFEEAVSWRKRQGYNSISFISAFPNWAADEHGATFANKDG
                     VYLRNAWEKFGSWAPNAKISTADGATTTAKDMHDEQGNRPFEVFADREGLANFDRINP
                     KYFASLDRKMRHLSEQGFVPFLETIRRDNAPSWKRYFDFNESYARFVQYLIARYGAYN
                     LVFSGIHLDWIPKDFSLTAEEFNEALTFHHRKYGGLPFGQPYTTLIDRSTYKVFGHGS
                     QAPWLTMHTVGNNPRNHAIYASLEELFRLEPAYPAANLEPYYTGWNHSINRPGGESPA
                     ENSDRDNYFARAMMYGSVLSGALAGHVHGTAAYDVTSTGEPAGWRPHIWTALRYESGA
                     QMRHLKTFVLSEGPRYQELTLASQDLETRSIPDALADGLDGWSFLMRTPQADFALVYF
                     ENRAPATRLRGFTPGANYRWRWFDPREGRWGAELRIKA"
BASE COUNT          484 a          820 c          756 g          426 t
ORIGIN      
        1 aaagccctcg gcgatgagca tgacaccgac gagcagcagg aacgacagcg ccagcatctt
       61 gatggacgga tgcgccgaga cgaaatcccc gatcgcgcgc gcgaacagca tcatgagtcc
      121 gaccgacgcg atgaccgccg cgatcatgac ggagacctcg tcgaccatgc cgacggcggt
      181 gatgatcgag tcgagcgaga acaccatgtc gatgatgatg atctgcagga tgatggcgcc
      241 gaagctcgcc ttcacgctct tggaggcatg accttcctca ccctcggtga gctggtggat
      301 ttccatggtg gatttccaga ccaggaacac gccgccggcg atgaggatca gatcccggcc
      361 ggaaatctcc gtcccggtga tggtgaacaa cggtgccgtc agccccacga tccacgacag
      421 caccatcagc aggccgatgc gcatgaacat cgcgaggaac aggccgatgc ggcgcgcttt
      481 ttcccgctgc acgcgctcga gcttgtcgac caggatcgag atgaaaatga tgttgtcgat
      541 gccgagcacc agctcgagcg ccgtcaacgt gaaaaaagcg atccatacct gcggatcgga
      601 caggagggcg gcgatggcat ccatgagtgc cgccatatta gccgaggctc gcgaacgagg
      661 tacccttcgt cacatgctca aactcatcat caggctcttc gcggctttcg tattcctgtc
      721 gctggccatg ccggccaccg cgcggctgtt gtccggcgag gttcacgtct gggagataca
      781 ggaaatcacg ctcgcggcca cgcgggacta cgcgaatccg tacgccgaca tcgagtgctg
      841 ggtggagctc gaagggccgg acttccgtcg ccgcgtgtac gggttctggg atggcggccg
      901 cacgttcaag gtccgcatcg tggcgactca gcccggtgac tggacgtggc gcgcgggctc
      961 gaaccgcgac gatccgggtc ttgccggttc gggaaaattc cgcgccgtgg cctggagcga
     1021 ggccgagcgc gcgcagaatc cgaatcggcg cggcttcgta cgcgccagtg aaaacggcca
     1081 tgcgttgcgt tatgccgatg gcacgccgtt ctttctcgtt ggcgacacct ggctcgccgc
     1141 ctccacctgg cgcctgccgt tcaagggtgt ccgcgctgcc gacgactctg tgccggccga
     1201 aggcatcagc ttcgaagaag cggtgagctg gcgcaaacgc cagggttaca actccatttc
     1261 gttcatctcc gcattcccga attgggccgc cgacgaacat ggcgccacct tcgcgaacaa
     1321 ggacggcgtg tatctgcgaa acgcctggga gaagttcggc tcctgggcgc cgaacgcgaa
     1381 gatctccacc gccgacggcg cgaccacgac cgccaaggac atgcacgacg agcagggcaa
     1441 tcgtccattc gaagtgttcg ccgatcgcga aggcctcgcc aacttcgatc gcatcaaccc
     1501 gaagtatttc gcaagtctcg atcgcaagat gcggcacctg tccgagcagg gcttcgtgcc
     1561 tttcctcgag acgatccgcc gtgacaacgc gccttcatgg aagcgctact tcgacttcaa
     1621 cgaatcgtac gcgcgcttcg tgcaatacct catcgcccgc tacggcgcct ataacctggt
     1681 gttcagcggc attcacctcg actggattcc caaggatttc agcctcaccg ccgaagaatt
     1741 caacgaggcg ctgaccttcc accaccgcaa gtacggcggc ctgccattcg gccagcccta
     1801 caccacgctc atcgaccgtt cgacgtacaa ggtgttcgga cacggatcgc aggcgccctg
     1861 gctcaccatg cacacggtgg gcaacaatcc gcgcaaccac gccatctatg cctcactcga
     1921 ggaactgttc cgcctcgagc ccgcataccc ggccgcgaac ctcgagcctt actacacggg
     1981 ctggaatcac agcatcaatc ggcccggggg cgagtcaccg gccgagaatt ccgatcggga
     2041 caactacttc gcgcgcgcca tgatgtacgg ctccgtgctg tcgggcgcgc tggccggtca
     2101 cgtgcacggc acggccgcgt acgatgtcac cagcaccggc gaaccggcgg gctggcggcc
     2161 ccacatctgg accgcattgc gctacgagtc cggcgcacag atgcggcacc tgaaaacctt
     2221 cgtgctttcc gaagggccgc gttaccagga actcacgctg gcctcgcagg atctcgagac
     2281 gcgcagtatt ccggacgccc tggcggacgg actcgacggc tggtctttcc tcatgcgcac
     2341 gccgcaagcc gatttcgcgc tggtgtattt cgagaaccgg gcgccggcca cgcggctgcg
     2401 tggcttcacc cccggggcta actaccgttg gcgctggttc gacccacgcg agggccgctg
     2461 gggcgcggag ctgcgcatca aggcgg
//
LOCUS       DATDEU010000155         7432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1485786, whole genome shotgun sequence.
ACCESSION   DATDEU010000155 DATDEU010000000
VERSION     DATDEU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485786"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2190
                     /locus_tag="VIV63_03840"
     CDS             <1..2190
                     /locus_tag="VIV63_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123758.1"
                     /translation="DTPVAVTALSAAALERQQVVSTTDLDKVAPNIQFHSYGTLTGNN
                     SAAQVFIRGIGQSDATPAVDPGVGVYIDEVYMGRAVGAAMEFRDIANVQILRGPQGTL
                     FGRNTIGGAVLLTTNEPGTDAGNTVRVGLGEDNLVEAFGAFDLPLGDVWSARLAVGMR
                     QRDGYVTRQFDGEDLGDEDMRTGQLAVRWKPSDAFSLTLRGDYTEEDENGSPFVFRTM
                     NEGATFVGAASIAAGCPNILDPFPPPLLVGPLDDPRCGNDAQALGEFVNGGTYQASST
                     LENRGVSLVADWDVNDTWSLKSITADRRLEWTGTRDADNTPLLILHTNYTSESKQFSQ
                     EFQALVDTDRLDGVIGVYYFDEDSNDRLLVPLGNPGTSYDTQRVAMDNEAKAAFTEWT
                     FKFTEAFSATAGIRYTEETKGLQATMFNVAPASAPEPAIPTALCPFAGAPPTQTGCLF
                     LTTNRFAREFTATTKSASVQYRFNESAMTYLSWSEGFKSGGFNQRYNAAPPGNAPISF
                     GAETAESFEIGLKLDPLDTLRINIAAFTTDYDDIQMTYRLGVVPLLFNAGVASIDGGE
                     LELEFAPTADFRLDAALGYLDAKFDSITPPPPFGPISPTATATLSSRLPFTPEWQGHV
                     GMSYSFHPGSNWTLTPRVDVSYTDTQFFDAGNSPEIAQDEAVTLLNASLTLASDDDKW
                     RFVLGGNNVTDELYPVAGTSSLTTASGYSEIIYSRPRSLTFYATRNF"
     gene            complement(2269..2751)
                     /locus_tag="VIV63_03845"
     CDS             complement(2269..2751)
                     /locus_tag="VIV63_03845"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEY6123759.1"
                     /translation="MNGKHTLAVACILMQLPAAAADDVPRIEFAFEERVTLAPAVVLG
                     NTALGHRQYIPITGGTVAGPKLKGQVVPGGFDFQLTYTASDCTQLSADYFLKAEDGTV
                     IHVFNEGLVCPGGERSLFRPRLEAPKGPHEWLTRATFVATLEVEPSGLAVRIRFYQVK
                     "
     gene            3024..4529
                     /locus_tag="VIV63_03850"
     CDS             3024..4529
                     /locus_tag="VIV63_03850"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY6123760.1"
                     /translation="MLKRLLLCAAMMSTLPVHALDGETQVHDPSTVILHAGRYYTYGT
                     GNGLPILTSDDGWTWRRSGSLMTAVPGGKAGDAVLARGGNNTWAPDIIHIGDKFFVYY
                     SAPATQPRSAIGLLVGRTLDPQSPDYQWEDGGPVVWSDGIEDSNAIDPGVLLAPDGRL
                     WLVYGSYFGYIRLVELNPKTGQRLDPDRKAVNVAINSEAAILIHRDGWYYLLVTHGSC
                     CQGANSTYNIRMGRSRKVTGPYLDNMGVDMIEGGGKLFAASRGRHVGPGHFGLLDLGQ
                     GVEKFSLHYEADLDRGGVSVLDIRPLLWRDGWPVAGDNLVAGTYAIESARTGTVLELA
                     VQGFPVGGFRARRPGGGTPGSGPPPEPPPAGPPVADQDPAQVAANWTSASGVRLAPAM
                     LQAQQKWVIAPVANAGGYPGAPYFRITIAGTERSLTATTGGELSAASFTGAPEQLWRI
                     DQLADGSYRIGPKSIPGISETMVLSAVGSSMPTLSRFNPASDRQRWLLGAP"
     gene            4529..5224
                     /locus_tag="VIV63_03855"
     CDS             4529..5224
                     /locus_tag="VIV63_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123761.1"
                     /translation="MSNAAKRYALTILFCLVGSGSMAQTPPPAETKPRPAAPTLTRPK
                     GATQLVDARGFIHRWLVLEPVPVSGRLTEPAVQEALKLAALPDAPGAQPSDGKTVTVN
                     GTDHRWHALDTSNYNVNLYHFAWALSKPTSNVLFWAQTTVDSPREMQGVRLAIGSNAA
                     SRWWLNGEPVISLNDDRQAVIDDGVSRRITLRKGRNVIRAAIVNGGGATDFCARFLDE
                     NDLPIQGLSVSLP"
     gene            5221..6708
                     /locus_tag="VIV63_03860"
     CDS             5221..6708
                     /locus_tag="VIV63_03860"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEY6123762.1"
                     /translation="MKRTHSFALALAVLLAPAANAAQSCGALSKFSMPGHRVLIRQAQ
                     EKEGVCRVDGVIDERTGRDGKSYGIGFALTLPVKWNGRFLFQGGGGLNGSVQPPVGGA
                     YAGERSALARGFAVASTDSGHQSTGFDASFFRDQQAALNFLYQAVAEVTVVSKAIIAR
                     HYGKPQQHAYFVGCSTGGREAMMMSQRFPDYFDGIVAAAPAARTSFSNLGLRHATAAL
                     NSIAPRGSDGKPQTRAAFSDADRTLLTDGILEACDSLDGNRDGLIFAPQKCAFDPQSL
                     ACAAGKKEGCLSGAQVTAIRAVMQGPRTSTGRQVYPGYFYDTGIATTQGLPGVLAGPV
                     IPEGRPVDTTFDVDAAAVEAMDARAMAGDSSAWTNLSTFRGHGGKLLFLHGVSDPWFS
                     AQETVRYYELLATDNPESPLPEWSRLFLVPGMGHCGGGARTLDQFDMLSAIVDWVESG
                     RAPERVIATGKSMPGESRPLCPYPRHAHFAGSGDARVETSYDCRE"
     gene            complement(6854..>7432)
                     /locus_tag="VIV63_03865"
     CDS             complement(6854..>7432)
                     /locus_tag="VIV63_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123763.1"
                     /translation="VATKPIRGVLPNPVTLYVDGRTNNLGTTIASGFDFMGTYEFEAG
                     AAGNFELGLSSTYFDTYEVAITPSADRLDQLNNIYNPLRFKARASATWSRGGILAGLF
                     INYLDAYTNNLVNPTEDVDSLSTVDLNISYTFQGGGFTEGLRIGLDAVNLLDEDPPFV
                     NIAQSPNGGGGFDPTLTNPVGRIIGLTIAKKW"
BASE COUNT         1327 a         2458 c         2319 g         1328 t
ORIGIN      
        1 gacacgcccg tggcggtcac cgcgctcagc gccgcggcgc tcgagcggca gcaggttgtc
       61 agcaccaccg atctcgacaa ggtggcgccg aacatccagt tccattccta cggaacactc
      121 accggcaaca actccgcggc ccaggtgttc attcgcggca tcggccagag cgatgccact
      181 cccgcggtcg atcccggcgt cggcgtgtac atcgacgagg tctacatggg ccgcgcggtg
      241 ggtgcggcca tggaattccg tgacatcgcc aacgtgcaga tcctgcgcgg cccgcagggg
      301 accctcttcg gccgcaacac cattggcggc gcggtcctgc tgaccaccaa tgaacccggg
      361 accgacgccg gcaacaccgt gcgcgtgggc ctgggcgaag acaacctcgt ggaagcattt
      421 ggcgccttcg acctcccgct gggtgatgtc tggtcggcgc gcctcgctgt gggcatgcga
      481 cagcgcgacg gatacgtgac ccgccagttc gatggcgagg atctgggcga cgaggacatg
      541 cgcaccggac agctcgcggt gcgctggaag ccctccgacg ccttcagtct cacgctgcgc
      601 ggggactaca cggaggaaga cgagaacggc tcgccgttcg tgttccgcac gatgaacgag
      661 ggagccacct tcgtcggcgc cgccagcatc gcggcgggct gtcctaacat cctcgatccg
      721 ttcccgccac cgttgctggt gggcccgctc gacgatccgc gctgcggcaa cgacgcgcag
      781 gcgctcggag aattcgtgaa tggcggcacg taccaggcga gcagcacgct cgagaatcgc
      841 ggcgtgtccc tcgtagccga ctgggacgtg aacgacacct ggtcgctgaa gtcgatcacc
      901 gcggaccgcc gtctcgaatg gaccggcacc cgcgacgccg acaacacgcc gctgctgatc
      961 ctccatacca actacaccag cgagagcaag cagttcagcc aggaattcca ggcgctggtc
     1021 gacaccgatc gcctcgatgg cgtcatcggc gtctactact tcgacgaaga ttccaacgac
     1081 cggctgctgg tcccactggg caatcccggc acgtcgtatg acacgcaacg cgtcgccatg
     1141 gataacgagg cgaaagcggc attcaccgag tggacgttca agttcaccga agcgttcagc
     1201 gcgaccgccg gcattcgcta cacggaagaa accaagggcc tgcaggccac gatgttcaat
     1261 gtcgcgcccg ccagcgcgcc ggaaccggcc ataccgacag cgctatgccc gttcgccggc
     1321 gcgccaccca cgcagaccgg atgcctcttc ctcaccacca atcgctttgc gcgcgaattc
     1381 acggcgacga cgaagtcggc cagcgttcaa tatcgcttca atgaaagcgc gatgacctac
     1441 ctcagctggt cggaaggctt caagagcggt gggttcaatc agcgctacaa cgccgcaccg
     1501 ccggggaatg cacccatctc cttcggcgcc gaaacggcgg aatcgttcga aattggcttg
     1561 aagctcgatc cgctcgatac gctgcgaatc aatatcgccg cgttcaccac ggactacgac
     1621 gacatccaga tgacgtatcg cctgggcgtg gtgccgctgc tgttcaacgc cggtgttgcc
     1681 tcgatcgatg gcggcgagct cgagctggag ttcgcgccca ccgcggactt ccggctggac
     1741 gccgcgttgg gttatctcga cgcgaagttc gacagcatca cgccaccgcc gccgttcgga
     1801 cccatctctc cgacggcgac cgccacgttg tcgagccgcc tgcctttcac gcccgaatgg
     1861 cagggacacg ttggaatgag ttacagcttc catccgggct cgaactggac actgactccg
     1921 cgcgtcgatg tcagctacac ggacacgcag ttcttcgatg ccggcaactc gccggagatc
     1981 gcgcaggatg aagccgtcac gctgctcaac gcctcgctga cgctcgcgtc cgacgacgac
     2041 aagtggcgct tcgtgctggg tggcaacaac gtcacggacg agttgtatcc ggtggcggga
     2101 acttcgtcgc tcacgacggc ttccggctat tcggagatca tttactcgcg tccgcgcagc
     2161 ctgactttct acgccacgcg caatttttga ccgcgcagtt tcccctgcgg gcaaaagtgc
     2221 ctcgccgcgg tgatggtcgg gctgacaatc accgcggcgc aggcagcatt acttgacctg
     2281 atagaagcgg atgcgcaccg cgagtccgga gggttcgact tcgagcgtgg ccacgaaggt
     2341 cgcgcgcgta agccactcat gcgggccctt gggcgcctcg agccgtggcc ggaacaaact
     2401 acgttcgccg ccggggcaca cgagtccttc gttgaacacg tggatcacgg tgccgtcctc
     2461 ggccttcagg aagtagtccg ccgacagctg cgtgcaatca ctcgcggtgt aggtgagttg
     2521 aaagtcgaat ccaccgggta cgacctgccc tttcaacttg gggcccgcca ccgttccgcc
     2581 ggtgatcgga atgtattgcc gatgtccgag tgccgtgttg ccgaggacga cggcaggcgc
     2641 gagcgtgacg cgttcttcga atgcgaactc gatgcgaggc acgtcatccg ccgccgcggc
     2701 aggcagctgc atcaggatgc aggccaccgc gagcgtgtgt ttgccattca cgatatgcag
     2761 tccatgcgcc gggacgttac gcggctggcg gcgcatcaac catccgcgga cacgtatccc
     2821 gacatgtcgc ttttcacatg catacgttat gcaagtcgct ttcatacaag caaccgggaa
     2881 tcggcctgaa tgggcaggct gggcgacctt tgtcgatggg cgcttcgaaa cgccgtcggt
     2941 tagcatgcag gctgacggtt caagggcgac ctgaaggcgc cgctgcctga ctaactacat
     3001 ccgcggaaat cggtgaccaa cccatgctga aacgattgct attgtgcgcg gcgatgatgt
     3061 ccacgcttcc cgtccacgcg ctcgatggcg agacgcaggt gcacgatccc tcgaccgtga
     3121 tcctccacgc tggccgctac tacacctacg gaaccggcaa cggactcccg atcctcactt
     3181 ccgacgatgg ctggacgtgg cgtcgttcgg gctcgctcat gaccgccgtg ccgggcggca
     3241 aggccggaga tgccgtcctc gcgcgcggtg gcaacaacac ctgggcgccg gacatcatcc
     3301 acatcggcga caagttcttc gtctactact ccgcgccggc cacgcaaccg agatccgcaa
     3361 tcggattgct cgtcggccgc acgctcgatc ctcagtcacc cgactaccag tgggaagacg
     3421 gtggcccggt agtctggtcc gatggcatcg aagacagcaa tgccatcgat ccaggcgtgt
     3481 tgctcgctcc cgacgggcgg ctgtggttgg tctacggttc ctacttcggc tacatccgcc
     3541 tcgtcgaatt gaatcccaag acgggccagc gtcttgatcc cgatcgcaag gcagtgaatg
     3601 tcgccatcaa ttccgaagcg gccatcctca ttcaccgcga tggctggtac tacctgctcg
     3661 tcacgcacgg ctcctgttgc cagggcgcca attccaccta caacatccga atgggccgct
     3721 cgcgcaaggt caccggcccc tatctggaca acatgggcgt cgacatgatc gaaggtggag
     3781 gcaagttgtt cgccgcgtcg cgcggtcgtc atgtcgggcc cgggcacttc ggccttctcg
     3841 acctgggtca gggtgtggag aaattttccc tgcactacga agcggacctc gatcgcggcg
     3901 gcgtgagcgt gctcgacatt cggccattgc tgtggcgcga cggctggccg gtcgccggcg
     3961 acaatctggt cgctggcaca tacgccatcg aatccgctcg taccggcacg gtgctcgagc
     4021 tcgctgttca aggatttccc gtcgggggat ttcgcgcgcg ccgtccgggt ggcggaacgc
     4081 cgggtagcgg tccaccgccc gagcctccac ctgcgggtcc gccggtcgcc gaccaggacc
     4141 ccgcgcaggt ggctgcgaac tggacgtccg cgagcggcgt gcgcctcgcg cctgcgatgt
     4201 tgcaggctca gcagaaatgg gtgatcgcgc cggtcgcgaa tgccggcggt tacccgggtg
     4261 ctccgtattt ccgcatcacc attgcgggga cagaacgctc gctcaccgca acaaccggcg
     4321 gtgagctcag cgctgcttcg ttcaccggtg cacccgaaca actctggcgc atcgatcagc
     4381 tcgccgatgg cagttatcgc atcggcccga agtccatccc cggaatctcc gaaacgatgg
     4441 tgctgtccgc cgtcggcagc agcatgccga cgctgtcccg attcaatccc gccagtgacc
     4501 gccagcgctg gctgctggga gcaccatgat gagcaatgcc gcgaaaagat acgccctgac
     4561 aatcctcttc tgcctggtgg gttcggggtc catggcgcag acccctccgc cagcggaaac
     4621 gaagccgcgc cccgccgccc ccacactcac gcgcccgaaa ggcgccacgc aactcgtcga
     4681 tgcgcgcggc ttcattcatc gctggctggt gctcgagccc gtgcccgtgt cggggcggct
     4741 gacggaaccc gcggtgcagg aggcattgaa gctcgccgcc ctgcccgacg cgccaggcgc
     4801 gcagccgtcc gatggaaaga cggttacggt gaacggcacg gatcaccgtt ggcatgcgct
     4861 ggacacctct aactacaatg tgaacctgta tcacttcgcc tgggcgctct cgaaacccac
     4921 ctcgaacgtg ttgttctggg cgcagaccac ggtggattcg ccgcgcgaga tgcagggcgt
     4981 gcgcctggcc atcgggtcga acgccgcctc gcgctggtgg ctcaatggcg agccggtgat
     5041 ctcgctcaat gacgatcgcc aggccgtgat cgatgacggc gtgtcgcggc gcatcacgct
     5101 gcgcaagggc cgcaatgtca ttcgtgccgc catcgtgaac ggcggcggcg ccaccgattt
     5161 ctgcgcccgt ttcctcgatg agaacgatct gccgatccag ggcctcagtg tgtcactgcc
     5221 atgaaacgca cgcattcgtt cgctctcgcg ctcgccgtat tgctcgcccc cgcggcgaac
     5281 gccgcgcagt cttgcggggc tctctcgaag ttttcgatgc cgggtcatcg cgttctcatc
     5341 cggcaggcac aggagaagga aggcgtttgc cgcgtcgacg gcgtcatcga tgagcgcacc
     5401 ggccgcgatg gcaaatcgta tggcatcggg tttgccttga cgctgcccgt caaatggaac
     5461 ggacgcttcc tgttccaggg cggcggcgga ttgaacggca gcgtgcaacc gccggtcggt
     5521 ggcgcatacg ccggcgaacg atcggcgctg gcgcgcggat tcgccgtggc gagcaccgat
     5581 tccggccatc agagcacggg tttcgatgcc agcttcttcc gcgatcaaca ggcagcgctc
     5641 aactttctgt atcaggcggt cgctgaggtg acggtcgtct ccaaggccat catcgcccgg
     5701 cactacggca agccgcagca gcacgcctac tttgtcggct gctccaccgg cggccgcgaa
     5761 gcgatgatga tgtcgcagcg attccctgac tacttcgacg gcatcgtcgc ggccgcgccc
     5821 gcggcgcgca ccagcttctc gaatctcggt ttgcggcacg ccaccgccgc gctcaactcc
     5881 atcgcgccgc gtggttccga cggcaagccg cagacgcgcg ctgcgttcag cgacgcggac
     5941 cgcacattgc tgaccgacgg cattctcgag gcctgcgact cgctcgacgg caatcgtgat
     6001 ggtttgatct tcgcgccgca gaagtgtgcg ttcgatccgc aatcgctcgc ctgcgcggcc
     6061 gggaagaaag aaggttgcct gtcgggagcg caggtgaccg cgattcgcgc cgtcatgcag
     6121 gggcctcgca cgtccaccgg gcgacaggtt tatcccggct atttctacga caccggcatc
     6181 gcgaccaccc agggcctgcc gggcgtcctc gccggacccg tcatcccgga aggccggccc
     6241 gtggacacca cattcgatgt ggatgcggca gcggttgaag cgatggatgc gcgcgccatg
     6301 gcaggcgaca gcagtgcctg gaccaatctc agcacgttcc gcggccacgg cggcaagctt
     6361 ctctttctcc atggtgtcag tgacccgtgg ttctccgcgc aggagaccgt tcgctactac
     6421 gaactactcg ccaccgacaa tcccgaatcg ccactgcccg agtggagccg tctgttcctc
     6481 gtgcctggca tggggcactg tggcggcggc gcgcgcacgc tcgaccagtt cgacatgctg
     6541 agcgccatcg tcgattgggt ggaatccggt cgcgcgccag agcgcgtgat cgcgacggga
     6601 aaatccatgc ccggagagag tcgcccgctc tgcccgtatc cgcggcatgc gcatttcgcc
     6661 ggcagtggcg atgcgcgcgt cgagacgagt tacgactgcc gagagtgagc cactgtgggg
     6721 atatacagtg ctgcgtgcac tcttatatat aggtcgcagg accactcggc cccgactgca
     6781 aaagtagggg cagcctgtcg gccaggctgc ccctgctagc ctcgcgttct accggggggg
     6841 cagtcgcgag gcgctaccac ttctttgcaa tggtcaggcc gatgatgcgg ccgacgggat
     6901 tggtaagcgt cggatcgaag ccgccaccgc cattcggact ctgcgcgatg ttcacgaatg
     6961 gcgggtcctc gtccagcaga ttcacggcat ccagaccgat cctcaaaccc tccgtgaatc
     7021 cgccaccctg gaaggtgtac gaaatattga gatcgacggt gctcagggag tcgacatcct
     7081 ccgtggggtt cacgaggttg ttcgtatacg catccagata gttgatgaac aggccggcca
     7141 ggatgccgcc cctgctccac gttgcactcg cacgcgcctt gaagcgaagc gggttgtaga
     7201 tgttgttgag ctgatcgagg cggtcggccg acggtgttat cgcgacttcg taggtgtcga
     7261 aatacgtgga gctgagtccc agctcgaaat tgccggccgc gcccgcctcg aattcatagg
     7321 tgcccatgaa gtcaaagccc gaggcgatcg tcgtacccag attgttagtg cggccgtcga
     7381 cgtacagcgt gacgggattc ggaagtacgc cgcggatagg cttggtggcg ac
//
LOCUS       DATDEU010000156         8166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1193940, whole genome shotgun sequence.
ACCESSION   DATDEU010000156 DATDEU010000000
VERSION     DATDEU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193940"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VIV63_03870"
     CDS             <1..996
                     /locus_tag="VIV63_03870"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY6123764.1"
                     /translation="VIEGVSPGHFPLGELLASGRPAVLRGVASNWGLVRAGLESGEQA
                     RRYLASFYNGKPIDFSFGEPHIGGRTFYTDDFTQLNFTVRRDRLDKVLGEIEAHLQDE
                     RPPTYYVASLVVDSCMPGFRKDNGLEFGPHGIDPPPSIWIGNRTIASCHYDAPNNIAC
                     CAVGRRRFTLFPPEQIINLYPGPLDPTPGGQAVSVVDFANPDFEKHPRFRDALAAGQS
                     AELEPGDAVFVPSMWWHHVEGLSPFNTLVNYWWSSVPDFMPTPMNALYHAIWTMRDRP
                     EREKQAWKNVLEYYVFGSSERAGAHLPEQARGVLGPIDDKQARHIRAMLMNKLNR"
     gene            1058..2572
                     /locus_tag="VIV63_03875"
     CDS             1058..2572
                     /locus_tag="VIV63_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6123765.1"
                     /translation="MNTHQVRRVVIAGGGTAGWLAAAALSHQFHGILDIVLVESEEIG
                     TVGVGESTIPPIRTFHRLLNIDEQEFMRATAATFKLGISFEDWGKIGDRYVHPFGSTG
                     RSTWACNFHHFWLHGLKKGIQSDFGEFCVEHQAAKAGKFFISPKTEINYAYHLDASVY
                     AKFLRKFSEKFGVRRVEGKIKDVRQNSESGFIEALTLESGQVVEGDLFIDCTGFRGLL
                     IEQTLKTGYEDWSHWLPCDRAVAVQTESVGPAVPYTRAIAHEAGWRWRIPLQHRVGNG
                     LVYCSRYMSDEQATEKLLREVEGKTIIQPRVIRFKTGRRLNAWNKNVIALGLAGGFVE
                     PLESTSIQMIMTGMTRLIYLFPFPTVTQSIIDQYNEESRIEAERVRDFIILHYCATQR
                     DDSEFWRYCSTMKLPDTLEHRIRLFRERAHVWQRDGEIFRTDSWIQVMLYQGIWPEHY
                     HYLATAMPDQDLVKFLEGMRSTINRAVDQMPTHQEFLDRYARASDAIWNMSAAK"
     gene            complement(2588..2983)
                     /locus_tag="VIV63_03880"
     CDS             complement(2588..2983)
                     /locus_tag="VIV63_03880"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEY6123766.1"
                     /translation="MSTTRRIFIAAAATVAATNAGASPAVNNIPKLAHHVFFWLKNPQ
                     SKEDLARLLEGLRTLAKIESVRAVHFGVPASTEKRDVVDNSYSASEILFFDDTAGQKI
                     YQDHPIHKQFVADCSHLWEKVVVYDAISA"
     gene            complement(2980..4443)
                     /locus_tag="VIV63_03885"
     CDS             complement(2980..4443)
                     /locus_tag="VIV63_03885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY6123767.1"
                     /translation="MSPPRLTEASLHSVRSGTILPTYDRGATRIGIVHIGPGAFHRAH
                     QAFYVDTLLHSDKRWAISALSLKSTGLRDALAQQQGLYTLVELGAAPRARVIGAIREL
                     LVGATDTDAAFTRLTARDTRIVSLTVTEKGYCLDATNQLDAANPDIAHDLANPGTPRS
                     TIGWLVEGLRRRRDQGIPPYAVLSCDNLPDNGTVLKRALVTFARRSDADLARWIEAEV
                     TCPRTMVDSITPATDDELRKRAQAITGVLDAWPIQREPFTQWVVEDVAVMRDADWASV
                     GVTLAKDVGVYDRAKLRLLNGPHSTLAYLGLLRGHESVADAMRDEQLAQFVELLMTED
                     LSPSLGAAPGLDVEHYISAVLARFRNPGIRHLLSQIAWDGTKKLPVRLVVTIADALRA
                     GRPIHRLLVPIAAWMRFVVRQAKAGIPIVDPDAARLASIGAACTGDARSDLPRFASCE
                     SVLPPALMSDERFRKELETAYDRLGSPQVALTPELSS"
     gene            complement(4462..6258)
                     /locus_tag="VIV63_03890"
     CDS             complement(4462..6258)
                     /locus_tag="VIV63_03890"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEY6123768.1"
                     /translation="MASDQNSQRPSRRFRSRDWFDDPKHLDMVALYLERFMNYGLTAN
                     ELLSGKPIIGIAQTGGDLTPCNRVHLDTVKRVREGIRDAGGVPMEFPLHPIFENCRRP
                     TAALDRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPAQVMAASTVDIPAIVLSGGPML
                     DGWHDGDLVGSGTVIWRSRRKLAAGQITEKQFLDAAAASAPSLGHCNTMGTASTMNAV
                     AEVLGLSLTGCAAIPAPYRERGQISYETGRRIVELAYEDLRPSKILTRESFLNAITAI
                     AAIGGSTNAQPHLVAMARHAGVEITPRDWMEFGRDIPLLVNMQPAGKYLGERFHRAGG
                     VPAVLSELLRVGKLDGKALTVTGHTLEQNIGEYIAARGAIDREVITTYEKPVQEKAGF
                     LVLKGNLFDFAIMKTSVISPEFRKRYLGNGDTFECRAVVFDGSTDYHDRINDPALGID
                     ENTILVIRGSGPLGWPGSAEVVNMQPPDALLKRGITSLPTLGDGRQSGTSDSPSILNC
                     SPESAAGGGLAWLRTGDKIRIDLAAGTCDMLVGEEEIARRKTAGLPPVPESATPWQEL
                     YRSTVGQLDSGAVMEMAVKYRGVAKKTPRHNH"
     gene            complement(6248..7120)
                     /locus_tag="VIV63_03895"
     CDS             complement(6248..7120)
                     /locus_tag="VIV63_03895"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6123769.1"
                     /translation="MSDIECVWPIGAILGEGPIWSATEQALWFVDIKAPAIHRFHPAS
                     GAQRSWPAPARVGFIAFTKTGGMLAGLKTGLHLFNPDTGNFTLLHVVEPHTPSNRLND
                     GFVDSRGYLWFGSMDDTEEEPSGALYQLTEAGCVRRDPGYVVSNGPAESPDGRVLYHT
                     DTLAGIIYAFDRAPDGALSSKRVFTRFGPGEGFPDGPIVDAEGCVWTGVFGGWGLRRL
                     SPKGELLSTLRLPCSAVTKAAFAGDDLKTLYITTAHVALDAEQRKQQPLAGGLFRARV
                     DVPGLPQGIVSHGI"
     gene            complement(7126..7878)
                     /locus_tag="VIV63_03900"
     CDS             complement(7126..7878)
                     /locus_tag="VIV63_03900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6123770.1"
                     /translation="MTHAVYPSLANKTVVITGGGSGIGEVMVEGFSRQKAKVFFLDVA
                     DGESKALVARLGNGVQFINTDVTDIEALRVSIASIEAKAGPVSVLVNNAANDDRHKFE
                     DVTPAYWEQRIAVNLRHIFFGAQFVTPGMKKAGGGSIINLGSVSWHSAIPELSIYETA
                     KAGIEGMTRALARDLGESNIRVNCVVPGAIRTPRQMKLWHTPEEEAKILAQQCLKARV
                     DPVHVSAMVLFLASDDARMCTAHNYWVDAGYR"
     gene            complement(7906..>8166)
                     /locus_tag="VIV63_03905"
     CDS             complement(7906..>8166)
                     /locus_tag="VIV63_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6123771.1"
                     /translation="DYDEALAVANAGEFGLSAGIMTTSLKYARHFVRNVRAGMVMTNL
                     PTAGVDYHVPFGGTRKSSFGPREQGFAAVEFYTQVKTSYTWA"
BASE COUNT         1496 a         2556 c         2636 g         1478 t
ORIGIN      
        1 gttatcgagg gcgtgagccc cggccatttt ccgctcggcg agttgctggc cagcggacgc
       61 cccgccgttc ttcggggcgt cgcaagcaat tggggcctgg tgcgcgcggg cctcgagtcc
      121 ggcgagcagg cgcggcgata cctggcttcc ttctacaacg gcaaaccaat cgacttcagc
      181 ttcggcgagc cgcatatcgg cgggcgcacc ttctataccg acgatttcac ccagctcaat
      241 ttcacggtac gccgcgaccg cctggacaag gttttggggg agatcgaagc ccacctgcag
      301 gatgagcgtc cgccgactta ctacgtggcc tcactcgtcg tcgattcctg catgccgggc
      361 tttcgcaagg acaacggcct cgagttcgga ccgcatggca tcgacccgcc gccgagcatc
      421 tggatcggaa accgcacgat cgcttcctgc cactacgacg cgccaaacaa tatcgcctgc
      481 tgcgcggtcg gccgccggcg attcacgttg tttccaccag agcagatcat caatttgtat
      541 ccgggtccgc tcgatccgac gcccggcggt caggctgtca gcgtcgtcga tttcgcaaac
      601 cccgatttcg aaaagcaccc gcgcttccga gacgcgctgg ccgcggggca gagcgcggaa
      661 ctggaaccgg gcgacgcggt gttcgtgccc agcatgtggt ggcatcacgt cgagggcctg
      721 agcccgttca atacgctcgt gaactactgg tggagctccg tgccggactt catgccaaca
      781 ccgatgaatg cgctctacca cgcgatatgg acgatgcggg atcggcccga gcgggaaaag
      841 caggcgtgga aaaacgtact cgaatactac gtgttcggat cgtcggaacg cgccggcgcc
      901 cacttgccgg agcaagcccg cggcgtgctg ggcccgatcg acgacaagca ggcccggcac
      961 atccgggcca tgctgatgaa taaactcaat cggtaaaccg gcgcgcgcgg ggcatactcg
     1021 cagcagctcc tgaacggata gacgtgagta aaaacccttg aacacgcatc aagtgcgccg
     1081 cgtggtaatt gccggcggcg gaaccgcagg ctggctcgcc gccgcggcgt tgtcgcacca
     1141 gttccacgga attctcgaca tcgtcctggt cgagtcggaa gaaatcggca cggtcggcgt
     1201 gggcgaatcc accatccctc ccatccggac gtttcaccgc ctgctcaaca tcgacgagca
     1261 ggagttcatg cgtgcgaccg cggccacgtt caagctggga atctccttcg aagattgggg
     1321 aaagatcggc gaccgatacg tgcatccgtt cggcagtacc ggcagatcga cgtgggcctg
     1381 caactttcat cacttctggt tgcacggcct caagaaagga atccagtcgg acttcggcga
     1441 gttctgcgtc gagcaccagg cagccaaggc cggaaaattc ttcatcagtc cgaagaccga
     1501 gatcaactac gcctatcacc tcgatgccag tgtctatgcc aagtttctgc gcaagttttc
     1561 cgagaaattc ggagtccgtc gcgtcgaagg caagatcaag gatgtccggc agaattcgga
     1621 atccggattc atcgaggcgc tgacgctgga atccggccag gtggtcgagg gcgacctgtt
     1681 catcgactgc accggttttc gaggcctgtt gatcgagcag acgttgaaga ccggctacga
     1741 agactggagc cactggctgc cgtgcgatcg tgcggtcgcg gtgcaaaccg aatcggtggg
     1801 tcccgccgtg ccgtacacgc gcgcgatcgc gcatgaggcg ggctggcgat ggcgcatccc
     1861 gttgcagcac cgcgtgggca acggactcgt ctattgcagc cgctacatgt ccgatgagca
     1921 ggcgaccgaa aaactgcttc gcgaagtcga aggcaagacc atcatccagc cgcgtgtgat
     1981 ccgcttcaag accggccgca ggctgaacgc gtggaacaag aatgtgatcg cgctgggact
     2041 cgccggcgga ttcgtcgagc ccctggaatc gacgagcatc cagatgatca tgaccggcat
     2101 gacccggctc atctatctat tcccgtttcc caccgtcacc cagtcgatca tcgatcagta
     2161 caacgaagag tcgcgcatcg aggcggaacg ggttcgcgac ttcatcatcc ttcactactg
     2221 cgccacccag cgcgacgaca gtgagttctg gcgttattgc tcgaccatga agctccccga
     2281 cacgctggag catcgcatcc ggctgttccg cgagcgcgcg cacgtgtggc agcgggatgg
     2341 cgagatcttc cgtacggatt cgtggatcca ggtaatgctc taccagggca tctggcccga
     2401 gcactaccac tacctggcga cggcgatgcc cgaccaggat ctggtgaagt tcctggaagg
     2461 catgcgcagc acgatcaacc gggcggtgga tcagatgccg acccaccagg aattcctcga
     2521 tcgctacgcg cgcgcgagcg acgccatctg gaacatgtcc gcggcgaagt agccgccgcg
     2581 gctcaggtca ggcactgatc gcgtcgtaca cgaccacctt ttcccacaga tgcgagcagt
     2641 cggcgacgaa ctgcttgtgg atgggatggt cctggtaaat cttctgaccg gcggtgtcgt
     2701 cgaagaacag gatctcggac gcgctgtagc tgttgtccac cacgtcgcgc ttctcggtgc
     2761 tcgcaggcac gccgaagtgc acggcgcgca cggattcgat cttggcgagc gtgcgcaggc
     2821 cttcgagcag ccgtgccagg tcttccttcg actgcggatt cttcagccag aagaacacgt
     2881 gatgagcgag cttcggaatg ttgttcacgg cgggggatgc tccggcgttg gtcgccgcaa
     2941 cggttgcagc ggctgcaatg aagattcttc gcgtcgtgct catgaggata actccggggt
     3001 aagtgccact tgcggcgagc caagcctgtc gtaagcggtt tctagttcct tgcggaaccg
     3061 ctcgtcgctc atcagcgcgg gaggcagcac ggactcacag ctcgcaaacc gcggcagatc
     3121 cgaccgcgca tcgccggtgc aggcggcgcc gatgctcgcc aggcgtgcgg catcgggatc
     3181 gacgatcgga attcccgcct tggcctggcg cacgacgaat cgcatccacg cggcaatcgg
     3241 caccagcagc cggtggatag ggcggccggc gcgaagcgcg tcggcaatcg tgaccacgag
     3301 gcggacgggg agcttcttgg tgccatccca ggcgatctgg ctcagcagat gccggatgcc
     3361 gggattgcga aatcgggcga gcaccgccga gatgtagtgc tcgacgtcga gcccgggcgc
     3421 cgcgccgagg ctcggactca ggtcttcggt catgagcagt tcgacgaact gcgcgagttg
     3481 ctcgtcacgc atggcatcgg cgaccgactc gtgcccgcgc aacagaccga gataggcgag
     3541 cgtcgaatgc gggccattca gcaaacgaag tttggcgcga tcgtagacac cgacgtcttt
     3601 cgccagcgtg actccgaccg aggcccaatc ggcatcgcgc atgaccgcca cgtcttccac
     3661 cacccactgc gtgaacggct cgcgctgaat cggccacgca tcgagaacgc cggtgatcgc
     3721 ctgcgcacgc ttgcgcagct catcgtccgt ggcgggcgtg atgctgtcga ccatcgtgcg
     3781 cgggcaggtc acttccgctt ctatccatcg tgcgagatcg gcatcggacc tgcgcgcgaa
     3841 cgttacgagg gctcgtttga gcaccgtgcc gttgtccggc aggttgtcgc agctcaacac
     3901 cgcgtaaggc ggaattccct ggtcgcgccg gcggcgcagt ccttccacga gccagccgat
     3961 ggtgctgcgc ggtgtgcccg gattggcaag atcgtgcgcg atgtcgggat tggcggcgtc
     4021 cagctgattc gtcgcgtcca ggcagtaacc cttctcggtc acggtcagcg agacgatgcg
     4081 ggtgtcgcgc gcggtgaggc gagtgaaggc cgcgtcggta tccgtcgcgc ccaccagcaa
     4141 ctcgcgaatc gcgccgatga cgcgtgcgcg cggcgcggcg ccgagctcga ccagcgtata
     4201 caggccttgt tgttgcgcca gggcatcgcg cagcccggtg ctcttcagcg agagcgcgga
     4261 aatcgcccag cgcttgtcgg agtgcagcag ggtgtcgacg tagaacgcct ggtgtgcccg
     4321 atgaaaggcg cccgggccga tgtgtacgat cccgatgcgc gtggcgccgc ggtcataggt
     4381 cggcaagatg gtgccggatc gaacggaatg cagactcgcc tcggtgaggc gcggcggact
     4441 catcagaacc tcactaacta atcaatggtt gtggcgcggc gtctttttgg cgacgccgcg
     4501 atatttcacc gccatttcca tgacggcgcc gctgtcgagt tgcccgaccg tggagcggta
     4561 cagctcctgc cagggtgtgg cgctttccgg cacgggcggc aggcccgcgg tcttgcgccg
     4621 cgcaatctct tcctcgccca ccagcatgtc acaggtgccg gccgcgaggt cgatgcggat
     4681 cttgtccccg gtccgcagcc acgcgagtcc gccacccgcg gcgctttccg gagagcaatt
     4741 gagaatggaa gggctgtcag acgtcccgga ctgccggccg tcgcccagcg tcggaagact
     4801 cgtgatgcca cgcttgagca gcgcgtccgg cggctgcatg ttcaccactt ccgccgaacc
     4861 gggccagccg aggggtcccg agccgcgaat cacgagaatc gtgttctcgt cgatcccgag
     4921 cgccgggtcg ttgatccggt cgtggtagtc ggtggagccg tcgaacacca ccgcccgaca
     4981 ctcgaacgta tcgccgttgc cgaggtagcg cttgcgaaac tccggcgaaa tgacgctggt
     5041 cttcatgatg gcgaagtcga agagatttcc cttcaacacg aggaagccgg ccttttcctg
     5101 cacgggcttt tcgtaggtcg tgatgacctc gcggtcgatg gctccgcgcg cggcgatgta
     5161 ttcgccgatg ttctgctcga gcgtgtggcc ggtaaccgtc agcgccttgc catccagttt
     5221 gccgacacgc agcagttcgc tgagcactgc cggcacaccg ccggctcggt ggaatcgctc
     5281 gcccaggtat ttgcccgcgg gctgcatatt cacgagcagc gggatgtcgc gcccgaattc
     5341 catccagtcg cgcggcgtga tctcgactcc ggcgtgacgc gccatcgcaa ccaggtgcgg
     5401 ctgcgcattc gtcgagccac cgatcgcggc aatggccgtg atcgcgttga gaaacgactc
     5461 gcgcgtgagg attttcgacg gccgcagatc ctcgtaggcg agttcgacga tgcgccgccc
     5521 ggtctcgtac gagatctggc cacgctcgcg ataaggcgcg ggaatggccg cgcatccggt
     5581 cagcgagagt ccgagtactt cggcgacggc gttcatcgtg gaagccgtgc ccatggtgtt
     5641 gcaatggccg agtgagggcg ccgaggcggc agccgcgtca aggaattgtt tttcggtgat
     5701 ctggcccgca gcgagcttgc gacgcgaacg ccagatcacg gttccggaac cgacgagatc
     5761 gccatcgtgc cagccatcga gcatcgggcc gcccgaaaga acgatggccg ggatgtcgac
     5821 ggtcgacgcg gccatgacct gcgcaggcgt cgtcttgtcg catccggtgg tgagtactac
     5881 cgcgtcgatc gggtagccgt gcaggatttc gacgagcccc agataggcca ggttgcgatc
     5941 gagcgccgcg gtagggcgac ggcaattctc gaagatcgga tgcagcggaa attccatcgg
     6001 cacgccgccg gcatcgcgga tgccttcgcg cacgcgcttc accgtatcga ggtgaacgcg
     6061 attgcaaggc gtgagatcgc cgccggtctg ggcgatgccg atgatgggtt tgcccgacag
     6121 cagttcattg gccgtgaggc cgtagttcat gaagcgctcg agatacagcg ccaccatgtc
     6181 gagatgtttc ggatcgtcga accagtcgcg cgagcggaag cggcgggacg gtctttgcga
     6241 attctgatca gatgccatgc gacacgattc cctgtggcaa ccccggaacg tccacgcgag
     6301 cgcggaacag cccaccggcc agcggttgct gcttgcgttg ttcggcgtcg agcgcgacgt
     6361 gggcggtggt gatgtaaagc gtcttgagat cgtcgcccgc gaaggcggcc ttggtcacgg
     6421 ccgagcaggg cagtcgaagg gtcgagagca actcgccttt gggcgacagg cgccgaagtc
     6481 cccagccgcc gaacacgccg gtccacacgc aaccttccgc atcgacgatg ggtccatcgg
     6541 gaaaaccctc gccgggtccg aagcgcgtga acacgcgttt gctggacagc gcgccatccg
     6601 gagcacgatc gaacgcgtag atgatgccgg ccagcgtatc ggtgtgatac agcacgcggc
     6661 cgtcggggct ttcggccgga ccgttcgaca ccacgtagcc cggatcgcga cggacgcatc
     6721 ccgcttcggt gagttggtac agcgctccgc tcggctcttc ctcggtatcg tccatcgagc
     6781 cgaaccacag gtatccgcgc gagtcgacga agccgtcgtt cagacggtta ctcggcgtat
     6841 gcggctccac gacgtgcaac agcgtgaagt tgcccgtgtc ggggttgaag agatgcagtc
     6901 cggtcttgag accggccagc atgccgccgg ttttggtgaa cgcgatgaag ccgacgcgcg
     6961 ccggcgcggg ccaggagcgc tgggcgccgc tcgcgggatg gaatcgatga atcgccggcg
     7021 ccttgatatc gacgaaccac agcgcctgct cggttgccga ccagatcggg ccctctccga
     7081 ggattgcgcc tatcggccag acacactcga tgtcggacat gactcttagc ggtaacccgc
     7141 gtccacccag tagttgtgcg cggtgcacat ccgggcgtcg tcggatgcca ggaacaacac
     7201 catggcggag acgtggaccg gatcgacgcg cgccttgagg cactgctggg cgaggatctt
     7261 ggcctcttct tccggcgtgt gccagagctt catctggcgc ggcgtgcgga tcgcgccggg
     7321 aacgacgcaa ttgacgcgaa tgttcgactc gccgagatca cgggccagcg cgcgcgtcat
     7381 gccttcgatg ccggccttgg ccgtctcgta gatcgaaagt tcgggaatcg ccgaatgcca
     7441 cgacaccgag cccaggttga tgatcgatcc gccgccagct ttcttcatgc cgggcgtgac
     7501 gaattgcgcg ccgaagaaga tatgccgcag gttcacggcg atgcgctgtt cccaataggc
     7561 cggcgtcacg tcttcgaact tgtgccggtc gtcattcgcc gcgttgttca cgagcaccga
     7621 gaccgggccg gccttggcct cgatggaggc aatcgagacg cgcaacgcct cgatgtcggt
     7681 cacgtcggtg ttgatgaact gcacgccgtt accgaggcgc gcaaccagcg ccttcgattc
     7741 gccgtccgcg acatcgagaa agaagacctt ggccttctgt cgcgaaaatc cttccaccat
     7801 cacttcgccg ataccggagc caccgcccgt gatgaccacg gtcttgttgg cgagtgaggg
     7861 ataaacagcg tgcgtcatac tgatctttga acccttcttg gaactctagg cccaggtgta
     7921 gctagtcttg acctgcgtgt agaactcgac cgcagcgaaa ccctgttcgc gcggaccgaa
     7981 gctcgacttg cgggttccgc cgaacgggac gtggtagtcc acgccggcgg tcggaaggtt
     8041 cgtcatgacc atgccggcgc gcacgttgcg cacgaaatgc cgcgcgtatt tgagcgaggt
     8101 ggtcatgatg ccggccgaga ggccgaattc gcctgcgttc gcgacggcga gagcttcgtc
     8161 gtaatc
//
LOCUS       DATDEU010000157         7329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2069594, whole genome shotgun sequence.
ACCESSION   DATDEU010000157 DATDEU010000000
VERSION     DATDEU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069594"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VIV63_03910"
     CDS             <1..474
                     /locus_tag="VIV63_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123772.1"
                     /translation="RRREQRLAELPFVEAQRLRAEHLDSYPIWQAQLPRMTDERLAAL
                     ASYGIETAEDVLRMPETLSNVVHREWVDTLRAWAAALDAGYQVDAEVLRKAIASNEED
                     TKLQKEQSSLLRKLRSGPYQLHEARAEVTRRRNAAEQRFIEARRSLLRARSEPVT"
     gene            471..2396
                     /locus_tag="VIV63_03915"
     CDS             471..2396
                     /locus_tag="VIV63_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123773.1"
                     /translation="MSLHRASTGAVVHVGHVLGRGGEGAVHVVNGTPKLVAKLYHKPP
                     DAAKAEKLRLMTRSHSPGLLGVAAWPVDLLTDERGQVRGFLMQKISARQDAHRLYSPK
                     SRRRTFPGADFRFVVRAATNVARAFAQIHAAGHVIGDVNHGNALIGKDGTAVLIDCDS
                     IQVHERSRTFACDVGTPLFTPPELQGKAFRGLKRDQAHDRFGLAVLLFHLLFQGRHPF
                     AGVFAEGEMPLERAIAESRFAYGAKAESDGMTAPPGTLPLDTFGPDIAALFERAFAFP
                     GTAERPSAVEWIEPLQKLESKLAACNLRPRHFHPRDKECCWCDIEERTGARLFDDTQT
                     NSADVDAMTAEQLWNAIEGVEAPPPLETPEFAVKQEAALVGTNLFGRIRDGMFGGVLP
                     ILVGGGVAVALLGNSSASGSLVFLGLLTSGAVLMSLANSSGRHSRLRRAMRIEWESAI
                     SQWRAKGTAGPFSRARSKLEESRRTFISLGEQRKKELEKLTQRFLGRQREDFLDTFDI
                     SKAPLHEVTRAQVDKLWSRGIRSAGDLRRHRRKLPSLIPHAAIDELHTWASHCEQTFR
                     FDTNEPTFTAHAAKIEDKYQKERQAVLQQLRRGPAVLAEKREDVAMARARADEALREA
                     QEKLNRPRETKRETQDE"
     gene            2506..4254
                     /locus_tag="VIV63_03920"
     CDS             2506..4254
                     /locus_tag="VIV63_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123774.1"
                     /translation="MAKIYHEAPDPNKVSKLAVMARLAPAELLRIAAWPIDLLNDEHG
                     ATRGFLMRRVSSREDAHELYSPKSRRRAFPDADFRFVVHAAANVARAFAQMHSLGHVI
                     GDVNHGNALIGRDATVMLIDCDSFQVKDGESNFTCDVGVPLFTPPELQGQTFRGLERK
                     AEHDAFGLAVLLFHLLFAGRHPYAGSYADGEMPIERAIAESRFAYGSDAVARGMSAPP
                     ATLPLTAFGAPVAALFERAFAGPAVSRPTAVEWVEALTLVESTLTNCVAASVHQHPRG
                     LSCCWCELESRSSVAMFGRTARTASPIFDAGELKRLWQVIADEIVPPASKILRELYIE
                     VARARAADIKGGRRAIVLFVYVLVALMVASGLTSKLGVTWITLTTIGGFVLHRWLKRT
                     EGDSAKLQKELIQEMETLVSSESYNQVMTSLEKARRRLLKIQKDPASVMSGVAATHEL
                     ARREQILDSIPLTAEGAPALNSGERAALASSGVETVADVLAERSQLHHALRYAQVRDL
                     IQWAEARANELQLDPGVTAELKRQSAVEQALRAEANALLGELREGPHRLKHARDEILK
                     ARAQAELALTQGRHRQ"
     gene            4322..4999
                     /locus_tag="VIV63_03925"
     CDS             4322..4999
                     /locus_tag="VIV63_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6123775.1"
                     /translation="MTTLDQVPFNNVEFADNPEPRCACLLLLDTSGSMKGTKIAQLNA
                     GLTTFAEQLRADAMAAKRVEVAVVTFGPVRVVQNFVTADSFSPATLAAAGDTPMGAAV
                     ERAVALVSERKKAYRANGIGYYRPWIFLISDGEPTDNIAAATALIRKGETKKSFMFYS
                     VGVENADIGKLAQIAVRRPLRLKGLSFGQLFVWLSDSLSSVSRSQISDAPELTNPTAP
                     DGWAVAE"
     gene            4999..5769
                     /locus_tag="VIV63_03930"
     CDS             4999..5769
                     /locus_tag="VIV63_03930"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY6123776.1"
                     /translation="MLWRYASGQSTGSSHEKLGKPCQDRMACATANADATLIAVVADG
                     AGTAGLAHIGAEIAVATVSSVAQLGVRAGRDDYQDILREGAALARQRLIEAAHERKAE
                     PRDLACTLLAVIVAPIGGGALQIGDGVIVIGEQPLAWRWVFWPQKGEYANSTFFLSDE
                     KALTQAEVASLPDEVQDVALMSDGLEPLAMQFATRTAHEPFFRTVLSPLHAVAEKGEA
                     PLLSTGLSNLLASPGVRARTDDDASLVIATRRKTVHAT"
     gene            5896..>7329
                     /locus_tag="VIV63_03935"
     CDS             5896..>7329
                     /locus_tag="VIV63_03935"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY6123777.1"
                     /translation="MRLLHGFGGFTAIALSLAMAACGGGGKKPPPPPPNQAPVSNAGA
                     DQSVFKAAAVTLDGAASTDADSNPLSYRWTQTGGTAVILSSATASRPTFTAPTLSGAL
                     TFSLVVNDGRVDSTADTVQVMIANRAPVANAGADGTVNGGSLYTLDALGSADADLDAM
                     SFTWVQLAGPQVTLTTVGLGRVRFTAPIVNGRLDFAVTANDGEASSAQDIVSVIVGNS
                     LPNDAPVAYLDLGYRAPKRSPVSIYGYAYDPQGSPLTYSWTQVTGPTVTLTGAATPLL
                     SFTAPANPTTLEFELVVSDGYLSSEPARITILVENFAPQVMGVQLTPDAPRTLDDLLL
                     NAATEDPDGDPITITYTWRRNGVVVPSVTGTTYPASLTTRDDVIVATVTASDGTDSVA
                     VDATVSIADTPPTLSATAAPTDVNFGDVVSFQIVAGTDPDGDPIGDYIVTLGPANFAV
                     SNTGLVNWQASGPMFESTEDIAWGIGLS"
BASE COUNT         1349 a         2398 c         2352 g         1230 t
ORIGIN      
        1 cgccggcgcg agcaacgcct ggccgaacta ccgtttgtcg aagcccagcg cctgcgagcc
       61 gagcatctgg actcctaccc catctggcag gcacaactgc ctcgcatgac cgacgaacgg
      121 ctggcggcgc tcgcctcgta cggaatcgaa accgccgaag acgtactgcg aatgccggag
      181 acactttcga atgtcgtaca ccgggagtgg gtcgacacgt tgcgtgcatg ggcggcggcg
      241 ctcgacgccg gataccaggt ggacgccgag gtgttgcgca aggccattgc cagcaacgaa
      301 gaagatacga agttgcagaa agaacaatct tcgctgctgc gcaagctgcg ctccggcccc
      361 tatcaattgc acgaagcccg cgccgaagtc acgcgccggc gtaatgccgc cgagcagcgc
      421 ttcatcgagg cgcgccgttc gctgttgcgc gcaagatcgg agcccgtgac gtgagccttc
      481 atcgcgcttc gacaggcgcc gtcgttcacg tggggcatgt gctcggccgc ggcggcgagg
      541 gcgcggtgca cgtcgtcaac ggaacgccga aactcgtggc caagctctac cacaagccgc
      601 ccgatgccgc gaaggcggaa aagctcaggc tgatgacgcg cagtcattcg ccaggtttgt
      661 taggcgtggc ggcgtggccg gtcgacctcc tgaccgacga gcgcggccag gtccgtggct
      721 ttctcatgca gaaaataagc gcgcgccagg acgcgcaccg gctctacagc ccgaagagcc
      781 gccgccgcac gtttcccggc gcggacttcc gcttcgtggt gcgcgcggcc accaacgttg
      841 cgcgcgcgtt cgcgcagatt cacgccgcag gtcacgtgat cggtgacgtg aaccacggca
      901 acgcgctcat cggcaaggac ggcaccgccg tgctcatcga ttgcgattcc atccaggtgc
      961 acgagcgcag ccgcacattc gcctgcgatg tgggaacgcc gctgttcacg ccgccggaac
     1021 tgcagggcaa ggcgtttcgc ggcctgaagc gcgaccaggc ccacgaccgc ttcggactcg
     1081 cggtcctgct gtttcatctg ctgttccagg gccgccatcc cttcgcgggc gtgttcgcgg
     1141 aaggtgagat gccgctcgag cgcgcaatcg ccgaatcgcg cttcgcctat ggcgcgaaag
     1201 ccgaatcgga cggcatgacc gcgccacccg gcacgttgcc actcgatacc ttcgggcctg
     1261 acatcgcggc gttgttcgag cgtgcattcg cgtttccggg taccgcggag cgtccctccg
     1321 cggtcgagtg gatagagcca ctgcagaaac tcgaaagtaa actggcagcc tgcaatttgc
     1381 ggccgcgtca ctttcaccct cgcgacaaag agtgttgctg gtgcgacatc gaggagcgga
     1441 ccggcgcgag gctgttcgac gatacgcaga caaattcggc cgacgtcgac gcaatgacag
     1501 ccgagcaact gtggaacgca atcgagggtg tggaagcgcc tccgccactc gaaaccccgg
     1561 agttcgcggt caagcaggaa gcggcgctgg tcggcacgaa ccttttcggc aggatccgcg
     1621 atggcatgtt tggcggtgtg ttaccaattc tcgtgggtgg cggcgtggcc gtggcacttc
     1681 tcgggaattc cagcgcttcc ggctcgctcg ttttcctggg gctgctgacg agcggcgccg
     1741 tcctcatgag tctggcaaat tccagcggtc gccactcgcg gctgcgtcgc gcgatgcgga
     1801 tcgagtggga aagcgcaatt tcgcagtggc gcgcgaaggg cacggctggc cccttctccc
     1861 gcgcgcgttc caagctggaa gaatcacgac gcacgttcat ctcgctcggc gagcagcgca
     1921 agaaggaact cgaaaagctc acgcagcgtt tcctcggtcg gcagcgcgaa gatttcctcg
     1981 acacgttcga catcagcaag gcgccgcttc acgaagtgac gcgagcgcaa gtggacaagt
     2041 tgtggagccg cggaattcga tcggcgggcg atctgcggcg gcatcgccga aagctcccga
     2101 gtctcattcc tcatgctgcc atcgacgagc tccacacctg ggcctcgcat tgcgagcaga
     2161 cgttcagatt cgatacaaac gaaccgacgt tcactgccca cgccgccaag attgaagaca
     2221 aataccagaa ggagcgacag gctgttctcc agcagcttcg ccggggcccg gcggtgctgg
     2281 cggagaagcg tgaagatgtg gcgatggcgc gggcgcgggc ggacgaagcc ttgcgcgagg
     2341 cgcaggagaa gctcaaccgt ccgcgcgaaa cgaagcgcga gacccaggat gaatagtttg
     2401 gccaggctcc ggcggcaatc gaacggcgca tccgtgtcgc tggggaagga actgggcagc
     2461 ggtggtgagg ggacggtcta tccagtcgaa gatgctccgg agctcgtggc gaagatctac
     2521 cacgaggcgc cggaccccaa caaggtttcg aagctggccg ttatggcgcg gctggctccc
     2581 gcagaactgc ttcgcatagc ggcatggccg atcgatctgc tgaacgacga gcacggcgcg
     2641 acacgcgggt tcctcatgcg ccgggtcagc tcgcgagagg acgcgcacga gctgtacagc
     2701 cccaagagcc gccggcgcgc gttcccggac gcggatttcc gcttcgtcgt gcatgctgcg
     2761 gccaacgtcg cgcgcgcatt cgcgcagatg cattcactag gccacgtgat cggtgacgtc
     2821 aatcacggca acgcgctgat cgggcgcgac gccacggtaa tgctcatcga ctgcgattcg
     2881 ttccaggtga aagacggaga gtcgaacttc acctgcgacg tcggcgtgcc gttgttcacg
     2941 ccgccggaat tgcaggggca aacctttcgc gggctcgagc gaaaagctga acacgatgct
     3001 tttggtctgg cagttcttct gtttcacctg ctatttgccg gccgacatcc ctacgccggt
     3061 agttatgccg atggcgagat gccgatcgag cgtgcgattg ccgaatcgcg gtttgcctat
     3121 ggttccgatg ccgtcgcccg tggcatgtcg gcgccgcccg cgacgctgcc cctcacggct
     3181 ttcggcgcgc cggtcgcggc actgttcgaa cgggccttcg cagggccggc agtctcccgg
     3241 ccgaccgcgg tcgagtgggt tgaagcactg acactggtgg agagcacgct gacgaattgc
     3301 gtggccgcgt cggtgcacca gcatccgcgc ggcctttcgt gttgctggtg cgagctggaa
     3361 agccgcagct ccgtcgcgat gttcgggcga acggcgcgca cggcgtctcc gatcttcgat
     3421 gccggcgagt tgaaacggct ctggcaggta atcgccgacg aaattgtacc gccggccagc
     3481 aagatcctcc gcgagctcta cattgaagta gcgcgggcga gagccgccga catcaaagga
     3541 ggtcggcgtg cgattgtcct gttcgtctat gtcctggtgg cgctcatggt cgcttctgga
     3601 ctcacatcga aactgggggt gacctggatc accctgacaa ccatcggcgg gttcgtgttg
     3661 caccgctggc tgaagcgcac ggaaggagac tccgcgaagc tccagaagga attgatccag
     3721 gagatggaaa cgctggtttc cagtgagtcg tacaaccagg tgatgacgtc gcttgaaaag
     3781 gcgcggcgcc gattgttgaa aatccagaag gatccggcgt cggtcatgag cggagtggct
     3841 gcgacgcacg agttggcacg gcgcgagcaa atcctcgact ccattccgct gactgccgag
     3901 ggagcgccgg cgctgaattc cggcgaacgc gcggcgctgg cttccagcgg tgtcgaaacc
     3961 gttgccgacg ttctggccga gcgcagccag ctacatcacg cgttgagata cgcccaggtg
     4021 cgcgacctca tccagtgggc cgaagcccgc gccaacgaat tgcagctcga tcccggcgtg
     4081 acagcggaat taaagagaca aagcgccgtc gaacaggcgc tgcgcgcgga agccaacgca
     4141 ttgctgggtg agttgcgaga gggtccgcac aggctcaagc acgcgcgtga cgagattctc
     4201 aaggcccgcg ctcaagcgga gcttgcactc acgcaaggca ggcatcgcca gtgaacgtgg
     4261 acgccaggcc ggaacgtgtt cactgttctt gaggccagca tctgcttacc atcctccgca
     4321 catgaccact ctcgaccagg ttcccttcaa caacgtggag ttcgcggaca accccgaacc
     4381 ccggtgcgcc tgcctgctgc tgctcgacac ctcgggctcc atgaaaggca cgaagatcgc
     4441 gcagctcaat gccggcctca cgacattcgc cgaacaactg cgcgccgacg cgatggccgc
     4501 caaacgcgtc gaggttgcgg tggtcacgtt cggaccggtg cgcgtcgtgc agaacttcgt
     4561 caccgccgat tccttttcgc cggcaaccct cgctgccgcc ggagacacac ccatgggcgc
     4621 ggccgtggag cgcgccgtcg cgctggtgtc agagcgtaag aaagcctacc gcgcgaacgg
     4681 catcggctat taccggccat ggatttttct gatctccgac ggcgagccca ccgacaacat
     4741 tgccgccgcc accgcgctga tccgcaaggg cgagaccaag aaatcattca tgttctattc
     4801 cgtcggcgtc gaaaacgccg acatcggcaa gctcgcgcag atcgccgtac ggcggccact
     4861 gcggctcaag ggactttcat tcggccagtt gttcgtctgg ctgtcggatt cgctgagcag
     4921 cgtttcgcgc tcgcagatct ccgatgcgcc cgagctcacc aacccgaccg cgcccgacgg
     4981 ctgggcagtg gccgaatagt gctctggcgc tacgccagcg ggcagtccac ggggagctcg
     5041 cacgaaaaac tgggcaagcc ttgtcaggac cgcatggcct gcgccacggc caatgcggac
     5101 gccacactca tcgccgtcgt ggccgacggc gccggcacgg cgggactcgc gcacatcggc
     5161 gcggagatcg cggttgcaac ggtgagcagc gtggcgcaac tcggcgtgcg cgcggggcgc
     5221 gacgactatc aggacatcct gcgcgaaggc gccgcgcttg cgcgtcagcg gctcatcgag
     5281 gccgcgcacg aaagaaaagc ggagccacgc gatctcgcct gcacgctgct ggccgtgatc
     5341 gtcgcgccga tcggtggcgg cgcgctgcag ataggcgacg gcgtcatcgt gatcggcgaa
     5401 caaccgcttg cgtggcgctg ggtgttctgg ccgcagaaag gcgaatacgc caactccacg
     5461 tttttcctga gcgacgagaa ggcgctgacc caggccgaag tcgcttcact gcccgacgaa
     5521 gtgcaggacg tggcgctgat gtccgacggg ctggaacccc tggcgatgca gttcgcgacg
     5581 cgaaccgcac acgaaccatt cttccgcacc gtgctgtcgc ctttgcacgc cgtggcggag
     5641 aagggcgaag cgccgctgct gtcgaccggg ttgtctaatt tgctggcatc tcccggcgta
     5701 agggcccgca ccgacgacga cgcctccctg gtcatcgcga cccgtcgaaa gactgttcac
     5761 gcaacataat tctccgctgt cactgaacgg tctcccgcag cacaaagcat atggggtaga
     5821 ttgcctgcga gatcggcccc caagagcagt aaagctgccg tgcagttgcc gggaagagtt
     5881 tcgggagtca aaagaatgcg cttgttacac gggttcggtg gtttcactgc gatcgcgctc
     5941 agcctggcga tggccgcctg tggcggaggg ggcaaaaaac caccaccgcc gccgcctaac
     6001 caggcgcccg tttccaacgc cggcgccgat cagtcggtct tcaaggccgc ggcggtcacg
     6061 ctcgacggag ccgccagcac ggacgccgac agcaatccac tgtcctatcg ctggacccag
     6121 accggcggca cggccgtcat tctgagcagt gccacggcgt cgcgccccac gttcacggcc
     6181 ccgacgctgt ccggcgcgct gacattctca ctcgtggtca acgatggccg cgtcgattcc
     6241 accgccgaca ccgtgcaggt catgatcgcg aaccgcgcac cggtggccaa cgccggcgct
     6301 gacggcacgg tcaacggcgg ctcgctctac acgctcgatg cgctgggcag tgccgacgcc
     6361 gatctggatg cgatgagttt tacctgggtc cagctcgccg gcccgcaggt cacgctcacc
     6421 accgtcggtc tcggccgcgt gcgcttcacg gcgccgatcg tcaatggccg gctcgacttc
     6481 gccgtcacgg ccaacgacgg cgaggccagc tccgcgcagg acatcgtctc ggtcatcgtc
     6541 gggaattcct tgcccaacga cgcaccggtg gcttacctcg acctgggtta ccgggcgccg
     6601 aagcgatccc ccgtatccat ctatggctac gcctacgatc cgcaaggcag ccctttgacg
     6661 tattcgtgga cgcaagttac ggggccgacc gtgacgctga ccggtgccgc cactccgctg
     6721 ctgtccttca ccgcgccggc gaatcccacg acgctcgaat tcgagctcgt ggtgagcgac
     6781 ggttacctga gttccgagcc ggcccgcatc accatcctcg tggaaaactt cgcgccgcag
     6841 gtgatgggcg tgcagctcac gcccgatgcg ccgcgtacgc tcgacgacct gctgctcaat
     6901 gccgcgaccg aagacccgga cggcgacccg atcacgatca cctatacctg gcggcgcaat
     6961 ggcgtcgtcg tgccgtcggt gaccggcacc acctacccgg cgagcctcac tacccgcgac
     7021 gacgtgatcg tcgcgaccgt cacggccagc gacggaaccg actcggtcgc ggtcgacgcc
     7081 acggtgtcga tcgcggacac gccgcccacc ttgagcgcca cggctgcacc gacggacgtg
     7141 aacttcggcg acgtggtgtc gttccagatc gtggccggca ccgatcccga tggcgatccg
     7201 atcggcgatt acatcgtgac gctcggcccg gccaacttcg ccgtgtccaa caccggactc
     7261 gtgaactggc aggccagtgg tcccatgttc gaatcgaccg aagacatcgc ctggggcatc
     7321 gggctcagc
//
LOCUS       DATDEU010000158         8855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_610693, whole genome shotgun sequence.
ACCESSION   DATDEU010000158 DATDEU010000000
VERSION     DATDEU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610693"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1900)
                     /gene="sdhA"
                     /locus_tag="VIV63_03940"
     CDS             complement(116..1900)
                     /gene="sdhA"
                     /locus_tag="VIV63_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY6123778.1"
                     /translation="MKDYEFIDHTYDVVVVGAGGAGLRATLGLAEAGLSTACITKVFP
                     TRSHTVAAQGGISAALGNMGEDDWRFHFYDTIKGSDWLGDQDAIEFMCKEAPAAVIEL
                     EHYGVPFSRTNDGRIYQRPFGGMTTHYGQGTAHRTCAAADRTGHAMLHTLYQQSIKHH
                     AEFFVEYFALDLIMQDGACRGVVALDLATGRLHRYRAHTVILATGGYGRAYFSCTSAH
                     TCTGDGGGMALRAGLPMQDMEFVQFHPTGIYGSGCLITEGSRGEGGYLTNSDGERFME
                     RYAPNAKDLASRDVVSRSMTIEIRGGRGVGKDKDHIHLHLEHLGPEVIHARLPGIAET
                     ARIFAGVDVNKDPIPVLPTVHYNMGGIPCNVHGEVVTLKSGNPDTVVPGLMAVGEAAC
                     VSVHGANRLGSNSLLDLVVFGREAGRHAATVVRPGQSHAPLAKDAGDFAVSRLDRFRN
                     ARGSRATAEIRLEMQKTMQRDAAVFRTGDTLKEGVTALAKTHASFADVKVADRSLVWN
                     TDLVETIELDNLLDQATATIVSAENRKESRGAHAREDFKDRDDSNWQKHTLCWVDEAG
                     KTRIEYRPVHMNTLTKDVEPIPPKARTY"
     gene            complement(1911..2288)
                     /gene="sdhD"
                     /locus_tag="VIV63_03945"
     CDS             complement(1911..2288)
                     /gene="sdhD"
                     /locus_tag="VIV63_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY6123779.1"
                     /translation="MSLRTPLGKVLGRGSAGDGVSHWWEQRVTAIALIPLTVWFVIGL
                     LQRSAGKFEDIQFWLSQPWSALLALLLVVTLALHSKLGVQVIIEDYVHGKGAKTTLLL
                     LSTFLHFAAAFAGIFAILRMALG"
     gene            complement(2285..2680)
                     /gene="sdhC"
                     /locus_tag="VIV63_03950"
     CDS             complement(2285..2680)
                     /gene="sdhC"
                     /locus_tag="VIV63_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY6123780.1"
                     /translation="MSQRPISPHIGVYKFAYTMALSILHRIMGGALSVGFLVFVWWLM
                     ALAQGPDAYAQAVDILRSGFFKLLLGGWALAFIYHFCNGIRHLFWDAGVGLERHQARR
                     SGTLVVIATVVLFALFLWAAYCYTPQVSG"
     gene            2941..3798
                     /locus_tag="VIV63_03955"
     CDS             2941..3798
                     /locus_tag="VIV63_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123781.1"
                     /translation="MSTLSGVDLTSPTDGLGVLAIRGADAARFLQGQLSADVEKLGPG
                     ASTLSGLHNPQGRVIALLLLLRASPEEVFAVLPRELVGAVEQRLRKYILRAKVRIEDS
                     SLTLKVVGSLTAPPANPDVPPVRWGDRWLALVPAGQALAETQTRAAWERADIAAGLPQ
                     VYLATSEAFVAQMLNLDLLDAIAFDKGCYTGQEVIARAHYRGRVKRRLQRWHSAESAA
                     PAVGASVRSADGRSLTVVRVAPGAEGGWDLLAVGPFEAATAPGESPDAGSSAAVSGPL
                     ALPYSLPAN"
     gene            complement(3795..4667)
                     /locus_tag="VIV63_03960"
     CDS             complement(3795..4667)
                     /locus_tag="VIV63_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6123782.1"
                     /translation="MRERSGVAHDRAVMPMRYGFADTLSGQLHFRACGAGPDIVLLPW
                     LPASGRMFEYVMPHLARAGFRAIAFDLAGSGRSHKKCRNWTVQQYAADVLEACGSLHT
                     VPYTVVGGRFSASVAVEMLLASPAQVTGAVLDGVPGLAPEELRKMTGPTTGLSPKISD
                     DGAHRSFAFDVSVRTLKDWDPSFELTAESLPLVYELMRDYLELGFESIAAGVDADAKG
                     GKATHDVLARLGEVSQRVLVMTSDADSLAPAYNRALERARNSRGHKFVGNHPVLTPAR
                     AAEYAQVVVSFARR"
     gene            complement(4740..5540)
                     /gene="murI"
                     /locus_tag="VIV63_03965"
     CDS             complement(4740..5540)
                     /gene="murI"
                     /locus_tag="VIV63_03965"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY6123783.1"
                     /translation="MNAPIGVFDSGVGGLTVLRALIAELPGEDFLYLGDTARLPYGTK
                     SPETVARYSVRAAEALESRGIKALVVACNTASATALPSLRERFPTLPVIGVIEPGAQA
                     ACEASVSGRIAVLATEGTVRGGAYERAILALRPDAHVTQVSAQVFVALAEEGWSEGEA
                     VDAVTERYLAHLDARVDTVVLGCTHFPLLVGAITRQLGPTRRVVDSAATTARATALVL
                     AARNLAAQSTRTGTVRLLATDSPERFARVGARFLGSSIAEKTVQAIDL"
     gene            complement(5537..7060)
                     /gene="lysS"
                     /locus_tag="VIV63_03970"
     CDS             complement(5537..7060)
                     /gene="lysS"
                     /locus_tag="VIV63_03970"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY6123784.1"
                     /translation="MSDDTSKPVAPVDENKLIAERRAKLAAWREQAKTAGIPAFPNDY
                     RRDVLASLLQAEYGDKPAEWFDANPPVRLHVAGRMMFQRIMGKASFAKLQDRTGQIQI
                     FLQRDALGDAYEAFKKYDVGDILAATGTLFKTKTGELSVRVEELRLLTKSLRPLPDKW
                     HGMADVDTRYRQRYVDLIVNEQSRNVFIVRNRILRYLRDFLDSMDFLEVETPMLQTIP
                     GGAAARPFKTHHNALDMDMFLRIAPELFLKRLTVGGLERVYEINRNFRNEGVSTRHNP
                     EFTMLELYVAYADYKDLIIMVERAMQGIADVVFGKRQFDYQGRTYDLDKPFTRITVID
                     AVAANVPGFDMTRARDIAYLRELCTRSGIHFKPGDGAGKLQIELFEKFAEDTFMEPTF
                     AYAYPAEVSPLSRANDADPFLTDRFEFFVAGRELANGFSELNDAEDQAARFRAQVERK
                     DAGDDEAMYYDADYVRALEYGMPPTAGLGVGIDRLVMFFTDAPSIRDVLLFPHMRPE"
     gene            complement(7057..8070)
                     /gene="prfB"
                     /locus_tag="VIV63_03975"
                     /pseudo
     CDS             complement(7057..8070)
                     /gene="prfB"
                     /locus_tag="VIV63_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(8165..8638)
                     /locus_tag="VIV63_03980"
     CDS             complement(8165..8638)
                     /locus_tag="VIV63_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6123785.1"
                     /translation="MTPATAASYSIREGDPLDPRVRELIAELDAFNSALYPAESNHFD
                     PPEALAAARAIFLVVEQDGELVGCGAAKNCGGWAELKRMYLKPKARGGGIALAMLVKL
                     LDWARAEKLPLARLETGNVSVGALKLYRRAGFKEIPAFPPYKPDPLSIFMERALR"
     gene            complement(8635..>8855)
                     /locus_tag="VIV63_03985"
     CDS             complement(8635..>8855)
                     /locus_tag="VIV63_03985"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123786.1"
                     /translation="IKNARVVGDKHLKMWVTTGDQSRNFDAIAFNFKGSDENPALPEG
                     DVRLVYRLDINEYKGERRLQLLVDHVLP"
BASE COUNT         1529 a         2819 c         2879 g         1628 t
ORIGIN      
        1 tgacgaactt gcgaacgcgc ttcgcacccg ccggcgccgg atatctcttg ccggcggcct
       61 tgtcgatctt cgagttcgcg ggaagagtga actcaaccat tccagtcttc cataatcaat
      121 acgtcctcgc cttcggcgga atcggctcga cgtctttcgt cagcgtgttc atgtgcaccg
      181 gccggtattc gatgcgcgtc ttgccggctt catcgaccca gcacaacgtg tgtttctgcc
      241 agttggagtc gtcgcggtcc ttgaagtctt cgcgtgcatg ggcgccgcgg ctttccttgc
      301 ggttctcggc gctgacgatg gtggccgtgg cctgatcgag cagattgtcg agctcgatgg
      361 tctcgacgag gtcggtgttc cagacgaggc tgcggtcggc gactttgacg tccgcgaagg
      421 acgcatgcgt cttcgcgagc gcggtgacac cttctttcaa ggtatcgccg gtacggaaca
      481 ccgccgcgtc acgctgcatg gtcttctgca tctcgaggcg gatctccgcg gtagcgcgcg
      541 aacccctggc gttgcggaag cgatcgagcc gcgacacggc gaaatcgccg gcatctttcg
      601 cgagcggcgc atgcgactgg ccgggcctga cgaccgtggc cgcatgacgc cccgcttcgc
      661 ggccgaacac cacgaggtcg agcaacgagt tgctgccgag gcgattcgcg ccgtgcacgg
      721 aaacgcacgc ggcttcgccc accgccatga gcccgggcac cacggtgtcg ggattgccgg
      781 atttcagcgt gaccacttcg ccatgcacgt tgcagggaat tccgcccatg ttgtagtgaa
      841 ccgtgggcag cacgggaatg ggatccttgt tcacgtcgac gcccgcgaaa atgcgcgcgg
      901 tctcggcgat gccaggcaga cgcgcgtgga tcacttcggg accgagatgc tcgaggtgca
      961 ggtggatgtg gtccttgtcc ttgccgacgc cgcgaccacc gcggatctcg atggtcatcg
     1021 accgcgagac gacgtcgcgt gacgcgaggt ccttggcgtt cggcgcgtaa cgttccatga
     1081 aacgttcgcc atcggaattg gtgagataac cgccctcgcc gcgcgagcct tccgtgatga
     1141 ggcagcccga gccgtagatg cccgtcggat ggaactgcac gaattccatg tcctgcatcg
     1201 gaagcccggc gcgaagcgcc atgccgccgc catcacccgt gcaggtgtgc gccgaggtgc
     1261 aggagaaata ggcgcggccg taaccgccgg tcgccaggat cacggtgtgc gcgcgataac
     1321 gatgcagccg gcccgtggcc agatcgagcg ccacgacgcc gcggcaggcg ccatcctgca
     1381 tgatgagatc gagcgcgaag tattcgacga agaattcggc gtgatgcttg atcgactgct
     1441 gatacagcgt gtgcagcatc gcgtggccgg tgcgatcggc ggccgcgcag gtgcggtgag
     1501 ccgtgccctg gccgtaatgc gtggtcatgc cgccgaaagg acgctggtag atgcggccat
     1561 cgttggtgcg cgaaaacggc acgccatagt gctcgagttc gatgaccgcg gccggcgctt
     1621 ccttgcacat gaactcgatg gcgtcctggt cgccgagcca gtccgagccc ttgatggtgt
     1681 cgtagaagtg aaagcgccag tcgtcttcgc ccatgttgcc gagcgccgcc gagattccgc
     1741 cctgcgccgc gaccgtgtgg ctgcgcgtgg ggaaaacctt ggtgatgcac gcggtggaca
     1801 ggccggcttc ggcaaggccc agcgttgcgc gaagtccggc gccgccggcg ccgaccacga
     1861 cgacgtcgta agtgtgatcg atgaattcgt aatctttcat gcgagatctt tcagccaaga
     1921 gccatgcgca ggatcgcgaa gatgccggcg aaggccgcag cgaaatgcaa gaacgtggag
     1981 agcagcagca gcgtggtctt cgcgcccttg ccgtgtacgt agtcttcgat gatgacctgg
     2041 acgccgagtt tcgaatgcaa ggcgagcgtc acgacgagca gcaacgccag cagcgccgac
     2101 cagggctgcg acagccagaa ctgaatgtcc tcgaatttgc cggcggaacg ctgcagcagt
     2161 ccgatgacga accataccgt gagcgggatc agcgcgatgg ccgtgacgcg ttgttcccac
     2221 cagtggctca cgccatcgcc ggcggaaccg cgccccagca cttttccgag cggcgtacgc
     2281 aggctcatcc ggacacctgc ggtgtgtagc aatacgcggc ccagaggaac agcgcgaaca
     2341 acacgacggt ggcgatcacg acgagggtgc cgctgcgccg cgcctggtgc cgttcgagac
     2401 ccacgcccgc atcccagaac aggtgacgga tgccgttgca gaagtgatag atgaacgcga
     2461 gcgcccaacc accgagcagt agtttgaaga agccggaacg caggatgtcg acggcctgcg
     2521 cataggcgtc cggcccttgc gcgagcgcca tcagccacca cacgaacacc aggaagccca
     2581 cggaaagcgc gccgcccatg atccggtgca ggatcgacag cgccatcgtg tacgcgaact
     2641 tgtacacgcc gatgtgaggt gaaatgggtc gctgactcat cgtggggaaa ccttagaagt
     2701 cgacgcagcg gccatttttt ttcccagtcg ccgaagcgcg taggctccgg accctgcggg
     2761 ccgccgattt caacgcgcag cgccttctcg gcggtctgca acgcgcctgc atcaggcacg
     2821 gcagccgtct ggtcgtcggt ggcgctgggc gggactagct cggaaggggc attcggcacg
     2881 ggcgcgaatt ctgccagaat cggccgcgcg cgaccacatg aaactcctgc aaaaacaaag
     2941 atgagcacac tcagcggcgt cgatctgacg agcccgaccg atggattggg cgtgctggcg
     3001 attcgcggcg ccgatgccgc gcgctttctg cagggtcagc tatcggccga cgtggagaaa
     3061 cttgggccag gcgcatccac gctttccggg ctgcacaacc cgcaaggccg cgtgattgcc
     3121 ttgcttcttc tgcttcgggc ttctcccgag gaagtgttcg cggtcctgcc tcgcgaactc
     3181 gttggagcgg tcgaacaaag attgcgcaag tacatcctgc gcgccaaggt ccgcatcgaa
     3241 gactcaagcc tcacattgaa ggttgttggc tcgctgaccg ccccgcccgc aaatcccgac
     3301 gtcccgcccg ttcgctgggg tgatcgctgg ctcgcgctgg taccggccgg ccaggcgctt
     3361 gccgaaacgc agacgcgcgc agcctgggaa cgcgctgaca tcgcggccgg cctgccgcag
     3421 gtgtacctgg cgaccagcga agccttcgtc gctcaaatgc tcaatctcga cttgctggac
     3481 gcgattgcgt tcgacaaggg ttgctacacg gggcaggaag tcatcgcgcg ggcgcactac
     3541 cgcggccgcg tgaaacgccg gctgcaacgt tggcactccg cggaatccgc ggcgcctgct
     3601 gtgggagcga gtgtgcgcag tgccgatggg cgttcgctca ccgtggtgcg tgtggcgccc
     3661 ggcgccgagg gcggctggga tctgctggcc gtcggcccgt tcgaggcggc cactgcgccg
     3721 ggagaatcac ctgacgcggg ctcgagtgcc gcggtgagcg ggcctctggc cctgccctat
     3781 tcgttgccgg ctaactagcg ccgcgcgaac gacaccacga cctgggcata ttccgcggca
     3841 cgcgccggcg tgagcaccgg gtggttgccg acgaacttgt ggccgcgcga attgcgagcg
     3901 cgttcgagcg cgcggttgta ggcaggcgcc agactgtccg catccgaggt catgaccagc
     3961 acgcgctgcg acacttcgcc gagacgcgcg agcacgtcat gcgtggcctt gccacccttc
     4021 gcatccgcgt cgacaccggc ggcgatgctc tcgaatccga gctcgagata gtcgcgcatc
     4081 aattcataga ccagcggcag cgattcggcg gtcagctcga atgatggatc ccaatccttg
     4141 agcgtgcgga cgctgacgtc gaaggcgaag ctgcgatgcg cgccgtcgtc ggagatcttg
     4201 ggcgagagcc cggtggtggg cccggtcatc ttgcgcagct cttcgggagc aagtcccggt
     4261 acgccatcga gcacggcgcc cgtcacctgc gcgggcgagg ctaacaacat ttccacggcc
     4321 accgaggcgg agaaacgccc gccgaccacg gtgtaaggaa cggtgtgcag cgagccacag
     4381 gcttcgagca catcggccgc gtattgctga acggtccagt tgcggcactt cttgtgggat
     4441 cgcccggagc cggcgagatc gaatgcgatg gcgcgaaagc cggctcgcgc gaggtgcggc
     4501 atgacgtact cgaacatgcg gccgctggcc ggtagccagg gcagcaggac gatgtcgggg
     4561 ccggcgccgc aggcgcggaa gtgcaactga cctgacaacg tatccgcgaa gccataacgc
     4621 atgggcatca cggcgcgatc gtgcgcaact cctgatctct ctctcatcag cccttccctt
     4681 gttctcggtc gccgcccgcg tgtcggggga gccaagtgta accgcgttat ggaaagtatc
     4741 taaagatcga ttgcctggac ggtcttttca gcgatcgatg agcccagaaa acgcgcaccc
     4801 acgcgcgcga atcgctccgg actgtcggta gccagcaggc gcacggtacc ggtgcgagtg
     4861 gattgtgccg caagattccg cgcagcgagc acgagcgccg tggcgcgcgc ggtcgttgcc
     4921 gccgaatcga ccacgcggcg cgtcggaccc aattgccggg tgatggcgcc gaccagcagc
     4981 gggaagtgag tgcagccaag caccaccgta tcgacgcgcg catcgagatg ggcgagataa
     5041 cgctcggtca cggcatcgac ggcttcgcct tcgctccacc cctcttcggc cagcgcgacg
     5101 aacacctgtg ccgaaacctg cgtgacgtgc gcatccgggc gcagggccag tatggcgcgc
     5161 tcatatgcgc caccgcgcac cgtgccttcg gtcgcgagca ccgcgatgcg tccgctgacg
     5221 gacgcttcgc aggccgcctg tgcaccgggc tcgatgacgc cgatgaccgg caatgtggga
     5281 aaccgctcac gcaggctggg cagcgcggtg gccgatgccg tgttgcacgc gacgaccagc
     5341 gccttgatgc cgcggctctc gagcgcctcg gccgcgcgta ccgaataacg cgccacggtt
     5401 tccgggctct tcgtgccgta cggaagacgc gcggtgtcac ccaggtagag aaaatcttcg
     5461 ccgggtagct cggcgatcag cgcgcgcagc accgtgagtc caccgactcc ggagtcgaac
     5521 acgccgatcg gcgcgttcat tcgggacgca tgtgcgggaa cagcagcacg tcgcgaatcg
     5581 acggcgcatc ggtgaagaac atcacgagac ggtcgatgcc tactcccagc cccgccgtcg
     5641 gcggcatgcc gtattcgagc gcgcgcacgt aatccgcgtc gtaatacatg gcttcgtcgt
     5701 cgccagcgtc cttgcgttcg acctgcgcgc ggaaccgcgc cgcctggtcc tcggcatcgt
     5761 tgagctcgga gaagccgttg gcgagctcgc gtccggcgac gaagaactcg aaccggtcgg
     5821 tgaggaacgg atcggcgtcg ttcgcgcggg acagcgggct cacttccgcg ggatacgcgt
     5881 aagcgaacgt gggctccatg aacgtgtctt cggcgaactt ctcgaacagt tcgatctgca
     5941 gcttgccggc gccatcgccc ggcttgaagt ggatgccgct cctggtgcac agctcgcgta
     6001 gataggcgat gtcacgcgcg cgtgtcatgt cgaagcccgg cacgttggcc gccaccgcgt
     6061 cgatgaccgt gatgcgcgtg aacggcttgt ccaggtcata cgtcctgccc tggtagtcga
     6121 actggcgctt gccgaacacc acgtcggcga ttccctgcat ggcccgctcg accatgatga
     6181 tgaggtcttt gtagtccgca tatgcgacat acagttcgag catcgtgaat tcggggttgt
     6241 gccgtgtcga cacgccctcg ttgcggaaat tgcgattgat ctcgtacacg cgctcaaggc
     6301 cgccgaccgt caggcgcttg aggaacagct ccggcgcgat gcgcaggaac atgtccatgt
     6361 cgagcgcgtt gtgatgcgtc ttgaacggac gcgccgccgc gccgccggga atggtctgca
     6421 gcatcggcgt ctcgacttcc aggaagtcca tggaatcgag gaagtcgcgc aggtaacgca
     6481 ggatgcgatt gcgcacgatg aacacgttgc ggctttgttc gttgacgatg aggtcgacgt
     6541 aacgctggcg ataacgcgtg tcgacgtcag ccatgccgtg ccacttgtcg ggcaacggac
     6601 gcaggctctt ggtgagcagg cgcagttctt cgacgcgcac cgacaactcg ccggtcttcg
     6661 tcttgaacaa cgtgccggtg gccgcgagga tgtcgccgac gtcgtatttc ttgaacgcct
     6721 cgtacgcatc cccgagcgca tcgcgctgca ggaagatctg gatctggccg gtgcggtcct
     6781 ggagcttggc aaagctcgct ttgcccatga tgcgctggaa catcatgcgg ccggccacgt
     6841 gcagtcgcac gggcgggttg gcgtcgaacc actccgccgg cttgtcgccg tattccgcct
     6901 gcagcagcga ggccagcacg tcacggcgat agtcgttggg gaacgccgga atgcccgccg
     6961 ttttcgcctg ctcgcgccac gccgccaact tcgcgcgccg ctccgcgatg agcttgttct
     7021 cgtcgaccgg cgctaccggc ttgctggtgt cgtcgctcac agtcccgcct tcaacgaagc
     7081 ttccatgaaa ggatcgagat tgccatcgag caccttgttg gtgtcaccga tttccactcc
     7141 cgtgcgcaga tccttgatgc gcgactggtc gagcacgtac gaccggatct ggttgcccca
     7201 gctcacgtcg ctcttggagt cttccagaac tttcgccgct gcattgcgtt tattgaattc
     7261 cagctcgtac atcttcgcct tcagcatttt catggccgtg gcgcggttct tgtgctggct
     7321 gcgctcgttc tggcaggcaa cgacaatgcc cgtggggccg tgcgtcaggc gcaccgccga
     7381 ttcggtcttg ttgacgtgct ggccgccggc accgctcgaa cgatagacgt ccatcttgat
     7441 gtcggccgga ttcaggtcga ccttgatgtc gtcgtcgacc tccggcgaga tgaacaccga
     7501 ggcgaacgag gtgtggcggc gattgccgga gtcgaatggc gacttgcgca ccagccggtg
     7561 tacgcccgtc tccgtgcgca gccagccata ggcgtagtca cccttgatct cgatggtggc
     7621 gctcttcatg ccggcgactt cgcccgcctg ggagtcgatg acctcggcgt cgaagccgcg
     7681 cgacgccgcc catttcagat acatgcgcag cagcatctgc gcccagtcct gcgcctcggt
     7741 gccacccgcg cccgcctgga tgtcgaggaa cgcgttgtgc gaatccatct cgccgcggaa
     7801 catgcgtttg aattcgagct tgcgcacgtc gctttcgagc tttgcggcat cgcgctcgat
     7861 ttcggtggcc atgccctcgt cgttctcgct ctcggcgaga tcgagtagtt cggaggcact
     7921 ggagatggcg ccgatggcct tgtccatgtc gacgaggtca gtcgtgaggc gtgcgcgttc
     7981 cttgcccagc tcctgggcaa tctccggctt gttccagatt gcggggtctt cgagctctcg
     8041 gcttacttct tcgagccgtt ctttcttttg gtcgtactca aagaaacccc ctcaaggaag
     8101 acatacgttc ctcgagatct ttgagctgac gcttcagcat gttgatttcc atagggcgcg
     8161 gagtctatct gagcgcgcgc tccatgaata tcgataacgg atcgggtttg tagggcggaa
     8221 acgcaggaat ttccttgaat cccgcgcgcc ggtagagctt gagcgcaccc acgctgacat
     8281 tgccggtttc gaggcgcgcc agcggcagct tttccgcgcg cgcccagtcg agcagcttca
     8341 ccagcatggc gagcgcgatt ccgccgccgc gtgccttcgg cttgaggtac atgcgcttca
     8401 gttccgccca gccgccgcag ttcttcgccg caccgcaccc gacgagctcg ccgtcctgct
     8461 cgaccaccag gaatatggcg cgggcggcgg ccagcgcctc cggcggatcg aagtgattgc
     8521 tctccgccgg gtacagcgcg gagttgaagg cgtcgagttc ggcaatgagc tcgcgcaccc
     8581 gcgggtcgag cggatcgccc tcgcggatcg agtagctagc agcggtggcc ggggtcacgg
     8641 aagcacgtga tcgacgagca gctgcaggcg gcgctcgccc ttgtattcgt tgatgtcgag
     8701 ccggtaaacc aggcgcacgt caccttcggg caacgccggg ttctcgtcgc tgcccttgaa
     8761 gttgaacgca atggcgtcga agttgcgcga ctgatctccc gtggtgaccc acatcttgag
     8821 atgcttgtcg cccactacgc gcgcattctt gatcg
//
LOCUS       DATDEU010000159         5926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2069647, whole genome shotgun sequence.
ACCESSION   DATDEU010000159 DATDEU010000000
VERSION     DATDEU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069647"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VIV63_03990"
     CDS             complement(<1..527)
                     /locus_tag="VIV63_03990"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEY6123787.1"
                     /translation="MPSRIKTERRTRSVPEGLWIKCSACDAVLYRAELERNLNVCPKC
                     SHHMRIGGRERLARFLDEGGQVEIGANVSPEDPLKFRDSKRYRDRLVAAQKATGERDA
                     MIVLAGTLEALPIVACAFEFKFLGGSMGSVVGERFKRGVDHCIANKAPLVCFSSSGGA
                     RMQEALFSLLQMAKT"
     gene            complement(564..1364)
                     /gene="truA"
                     /locus_tag="VIV63_03995"
     CDS             complement(564..1364)
                     /gene="truA"
                     /locus_tag="VIV63_03995"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY6123788.1"
                     /translation="MPRFAAGLEYDGRAYSGWQFQPGLKTVQDVVQRAFSRVADSPVA
                     CACAGRTDAGVHALAQVVHFDSTAVRSERGWRLGANSYLPDDVSVAWVREVPEHFHAR
                     FGATARSYRYLVFNRDSRSALASGRATWERRPLDAARMHAAAQVLLGEHDFSAFRAVE
                     CQAKSPVRHVERIEVTRTAEWLTLAITANAFLHHMVRNVTGLLLSVGHGESPPERVAT
                     VLASRDRKTNAATAPPDGLYLTAVRYPAEFGLPGGNGLQSAIIGGTFE"
     gene            complement(1365..4223)
                     /locus_tag="VIV63_04000"
     CDS             complement(1365..4223)
                     /locus_tag="VIV63_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY6123789.1"
                     /translation="MKRMGPARVLLAFLLLPGISHALGLGDVHLNSPLNAPLDAEIEL
                     VNATPEDLATLDAKLASKETFERYGLEWPQFMSTVTVTRDRSASGGQVLRIRSSETVT
                     EPFLTLLIEASWARGRLVREYTVLLDPPVFAPNSVVAAQPSMPSAGGAVASGQISRPA
                     QPEPQSAAPVAAGGGDSYEVQRGDSLSAIARRLSASSGAQTSQLMVSIYRANTGAFEG
                     DMNRLRAGSVLRIPSGEEIAAVSPSEASGEVRRLASGWSTSSGESGAGRLRLVPPSDS
                     AGNPGASAGNAAEVDELQGRVRELEGQLTESKRMLELRNTELADLQAKLAASQQQPAA
                     AQPAPAQTPVEQPLPQPEATAPVPEAVAPETPATQEPAPTPAETPVAKPERPKPVAAE
                     TEPEGPSLVDTLMGYWWVLAAGVVLLLALVFMKSRGKRKTAEFDDNLGRLADHGEDRL
                     AQPMDSLSDTGRMRAPNMESIPDDILVEESGTHRALQETQDIPLAPTVRTDETISSET
                     AVNLDQGDPLAEADFHMAYGLYDQAADLVRIAIQREPDRRDLKLKLLEVFFVWGNKEQ
                     FLSMARELSDTRSESGLGEWEKIVIMGKQIAPEDSMFSDGGAGSAVGVDLNLDAGGSG
                     RVDFDPFDVSARMDVTGGGNADAVDLDLSNALRDPDATGEGLQLPNNPASGQTTREMT
                     VKMTPAGSEAPTVEQPALRPLDEPTIREKVEGAMRRKLSSDQTAELALDDLGLELGSL
                     EQTDSQIGLKPVGGMPRGPDANAPTMVAGLDENSQRLLAAAAARHSGGNADDALTEHG
                     ASGTWFLTERELGGDVDLTKGRAIDPGSTASMAKFEMPEAGFDISSTSRLAAIDRENL
                     DFPVEPTQQQPSMGRGKVDLDIGNGFDMPGSATEELAVPDLEPVTLSEVGTKLDLARA
                     YVDMGDPDGARNILNEVLSEGSASQKQEAQRLLESLPG"
     gene            complement(4336..5382)
                     /locus_tag="VIV63_04005"
     CDS             complement(4336..5382)
                     /locus_tag="VIV63_04005"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY6123790.1"
                     /translation="MNEVKKDPPKGYRVCVLGATGLVGDMMIRILEERDFPVSELLPL
                     ASSRSVGKRVEFRGRHIPVIDVATFDFSRAQIGLFSAGGDVSKQYAPKAAAAGCIVID
                     NTSEFRYEDDIPLVVPEVNPKAIGEYKKRGIIANPNCSTIQMVVALKPIYDAVGITRV
                     NVATYQSVSGAGKEAVDELAAQTTKLMSGEEPDKPQVIAKRIAFNCVPQIDKFMDNGY
                     TKEEMKMVWETRKILGDPAILVNATAVRVPVFFGHSEAVHIETRQKITAEAARELLRR
                     APGVEVLDERKPGGYPTAATEAANRDTVYVGRIREDISHERGLNLWIVSDNIRKGAAT
                     NSVQIAEILVREHI"
     gene            complement(5387..>5926)
                     /locus_tag="VIV63_04010"
     CDS             complement(5387..>5926)
                     /locus_tag="VIV63_04010"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY6123791.1"
                     /translation="ELEDLMWNIRGDGDSLGAKVTVLARGVPPGLGEPVFDRLDADLA
                     HAMMGINAVKAVEIGAGVASAAQMGSVQRDELTPTGFLSNNAGGILGGISSGQDVLVH
                     VTFKPTSSIQVPGKTIDVAGNAVDVVTTGRHDPCVGLRATPICEAMLAIVLMDHYLRH
                     RGQNADVRCDTPVITGQGR"
BASE COUNT          973 a         1914 c         1967 g         1072 t
ORIGIN      
        1 ctggtcttgg ccatctgcag cagcgaaaac aacgcctcct gcatgcgcgc accgccactc
       61 gacgagaaac acacgagcgg cgccttgttg gcgatgcagt ggtcgacacc gcgcttgaag
      121 cgctctccga ccaccgaacc catggagccg ccgaggaact tgaattcgaa cgcgcaggcg
      181 acgatgggca gcgcttcgag tgtgcccgcg agaacgatca tcgcgtcgcg ctctcccgtg
      241 gccttctggg ccgcgacgag acgatcgcgg taacgcttgc tgtcgcgaaa cttcagcgga
      301 tcttcgggcg agacattggc gccgatctcg acctggccac cctcgtcgag gaatcgcgcc
      361 agccgttcgc ggccgccgat gcgcatgtgg tggctgcact tcgggcagac attgaggttg
      421 cgctcgagct cggcgcggta cagcacggcg tcgcaggccg agcatttgat ccacagaccc
      481 tcgggcaccg aacgcgtgcg gcgctcggtc ttgatgcgcg agggcatgat tttctcgaac
      541 caagacatgt cgcgcggctt cccctattcg aaagtgccgc cgatgatagc ggattgcagc
      601 ccgttgccgc ccggcagtcc gaactcggcc ggataccgca cggccgtgag gtacaggcca
      661 tccggcggcg cggtcgccgc attggtcttg cggtcgcggc tggcgagtac cgtggccacc
      721 cgctccggcg gcgattcgcc atggcccacc gacagcagca ggccggtcac gttgcgcacc
      781 atgtgatgca ggaaagcatt cgccgtgatc gccagcgtca gccactccgc agtgcgcgtg
      841 acctcgatgc gctcgacgtg ccgcaccgga gatttggcct ggcattccac cgcgcggaac
      901 gcgctgaagt cgtgctcgcc cagcaacacc tgcgccgcgg catgcatgcg ggccgcgtcg
      961 agcgggcgcc gttcccaggt ggcccggccc gaggccagcg ccgagcgcga gtcgcgattg
     1021 aagacgaggt atcggtaact gcgcgcggtc gcgccgaaac gcgcgtggaa atgttcgggc
     1081 acttcgcgca cccaggcgac gctgacgtcg tcgggtagat agctgttggc gccgagccgc
     1141 cagccgcgtt cgctgcgcac ggccgtgctg tcgaagtgaa ccacctgggc cagcgcgtgg
     1201 acaccggcgt cggtgcgacc ggcgcacgcg caggcgacgg gcgagtcagc cacgcgcgag
     1261 aacgcgcgct gcacgacgtc ctgaacagtc ttgagtcccg gctggaattg ccagccggag
     1321 tacgcgcgcc cgtcgtactc gaggccggca gcgaatcgag gcatttagcc gggcagcgac
     1381 tctaacaagc gctgcgcttc ctgcttctgc gaggccgagc cttccgacag cacttcgttg
     1441 aggatgttgc gcgcgccgtc cggatcgccc atgtcgacgt aggcgcgagc caggtcgagc
     1501 ttggtgccga cttccgagag cgtcaccggt tcgagatccg gaaccgcgag ttcttcggtc
     1561 gcgctgccgg gcatgtcgaa gccgttgccg atgtcgagat cgaccttgcc gcggcccatg
     1621 gacggctgct gctgcgtggg ttcgaccggg aaatcgaggt tctcgcggtc gatggccgcc
     1681 aggcgcgacg tcgacgagat gtcgaagccc gcttccggca tttcgaactt cgccatcgaa
     1741 gccgtgctgc ccgggtcgat ggcgcggccc ttggtcaggt ccacgtcgcc gccgagttcg
     1801 cgttcggtca ggaaccacgt gccgctggcg ccgtgttcgg tgagcgcatc gtcggcattg
     1861 ccgccgctgt ggcgcgcggc cgcggcagcc agcaggcgct gggagttctc atcgagaccc
     1921 gcgaccatgg tcggggcgtt ggcatcgggg ccacggggca tgccgcccac gggcttgagg
     1981 ccgatctgcg aatccgtctg ttcgagcgaa ccaagctcga ggcccaggtc gtcgagcgcg
     2041 agttccgcgg tctgatccga gctcagcttg cggcgcatgg cgccttcgac cttctcgcga
     2101 atcgtcggct cgtcgagcgg acgcagcgcg ggctgttcaa cggtcggcgc ttccgagccg
     2161 gccggcgtca tcttcacggt catctcgcgc gtcgtctggc cgctcgcggg attgttgggg
     2221 agctgcaggc cttcacccgt ggcgtccgga tcacgcaagg cgttgctgag atcgaggtcg
     2281 acggcgtcgg cattgccgcc accggtcacg tccatgcgcg cgctgacatc gaacgggtcg
     2341 aaatcgacac ggcccgaacc accggcatcg agattgagat cgacgccgac cgcgctgccc
     2401 gcgccgccgt cggagaacat cgaatcttcg ggggcaatct gcttgcccat gatcacgatc
     2461 ttttcccact cgcccagacc ggactcggag cgcgtatcgg aaagctcgcg cgccatggag
     2521 aggaactgct ctttgttgcc ccagacgaag aagacttcga ggagcttgag cttgaggtcg
     2581 cgacgatcgg gctcgcgctg gatggcgatg cgaaccagat cggcggcctg gtcgtaaaga
     2641 ccataggcca tgtggaaatc ggcttcggcc agcggatcgc cctggtcgag attgacggcc
     2701 gtctcgctgc tgatggtctc gtcggtacgg accgtcggag ccagcgggat gtcctgggtt
     2761 tcctgcaacg cgcgatgagt gcccgattcc tcgacgagga tgtcgtcggg aatggattcc
     2821 atgttcggcg cgcgcatgcg gccggtgtcg gacagcgagt ccatcggctg ggcaaggcgg
     2881 tcttcaccgt gatcggcgag acggcccagg ttgtcgtcga actcggcggt cttgcgcttg
     2941 ccgcgcgact tcatgaatac cagtgcgagc aacagcacca cacccgcggc cagcacccac
     3001 cagtaaccca tcagcgtatc gacgagcgac gggccttccg gctcggtctc tgcggctacg
     3061 ggtttcgggc gctccggctt cgcgacaggg gtttcggccg gcgtcggcgc tggctcctgc
     3121 gtggcgggcg tttcaggcgc gacagcctcc ggcacgggcg cggtcgcctc gggctggggc
     3181 aacggctgtt cgacgggagt ctgagccggc gcgggttgcg cggccgcagg ctgctgctgc
     3241 gaggccgcca gtttggcctg cagatcggcg agttcggtat tgcgcagctc gagcatgcgc
     3301 ttggattcgg tcagctggcc ttcgagttcg cgcacgcggc cttgcagctc gtcgacttcg
     3361 gcggcattgc cagccgaggc gccgggattg ccagcggagt cgctcggggg cacgaggcgc
     3421 agacggccgg cacccgactc accgctcgag gtggaccagc cactggcgag acggcgcact
     3481 tcgccactcg cttcggacgg agaaaccgcg gcgatttctt cgccgctcgg aatacggagc
     3541 accgatccgg cgcgcaggcg gttcatgtcg ccttcgaaag cgcctgtgtt ggcacggtag
     3601 atagacacca tgagctgcga ggtctgggca ccgctgctcg cggacaggcg gcgcgcgatc
     3661 gccgacaggc tgtcgccacg ctgcacttcg taactgtcac caccacccgc ggcgacgggg
     3721 gccgcgctct gcggttccgg ctgcgccgga cgggagatct ggccggaagc caccgcgcca
     3781 ccggcggaag gcatgctcgg ctgcgccgcg accacgctgt tgggcgcaaa cacgggcgga
     3841 tcgagcagaa cggtgtattc gcggaccagg cggccgcggg cccacgaggc ttcgatgagc
     3901 aaggtgagga acggctcggt gaccgtttca ctggatcgga tgcgaagtac ctggccgccg
     3961 ctggccgagc gatcgcgggt caccgtcacc gtggacatga actgcggcca ttcgaggccg
     4021 taacgttcga atgtttcttt cgatgcgagc ttcgcgtcga gcgtggcgag atcttcgggc
     4081 gtcgcgttga cgagctcgat ctccgcgtcc agaggcgcgt tgagcggcga gttcagatga
     4141 acgtcaccga gccccagtgc atgggaaatt ccgggcaaca gcaaaaaagc cagcaaaacc
     4201 cgggcaggac ccatccgctt cattcgcgtc tccactacca ccgtcggcga tccaatatct
     4261 gcgtaatttc tttatcgaaa catgagctta gcgccgaaac ttccggctta agcatgaaac
     4321 cgcccgcaat ataacttaaa tatgctctcg caccaaaatc tcggcgatct ggacgctgtt
     4381 cgtggcagca cccttcctga tgttgtccga gacgatccat aagttaagtc cgcgttcgtg
     4441 acttatatct tccctaatgc gtccgacata aaccgtgtcc cggttcgcgg cttcggtggc
     4501 tgccgtgggg taaccaccgg gcttccgctc gtctaacacc tcgacacccg gggctcgcct
     4561 gagcaattcg cgcgcggctt cggcggtgat cttttgccgg gtttcgatgt gcacggcttc
     4621 ggaatggccg aagaacacgg gcacgcgaac cgccgtcgcg ttcacgagga tggccgggtc
     4681 accgaggatc ttcctcgtct cccacaccat tttcatttct tccttggtgt agccgttgtc
     4741 catgaacttg tcgatctgcg gcacgcagtt gaaggcgatg cgcttggcga tgacctgggg
     4801 cttgtccggt tcctcaccgc tcatgagttt cgtggtctgg gcggccagtt cgtccaccgc
     4861 ttccttgccg gcgcccgaca cggattgata ggtggccacg ttcacgcgcg tgatgcccac
     4921 ggcgtcgtag ataggcttga gcgccacgac catctggatg gtcgagcagt tgggattggc
     4981 gatgatgccg cgtttcttgt attcgccgat cgccttcggg ttcacctcgg gaacgaccag
     5041 cgggatgtcg tcctcgtaac ggaactcgga ggtgttgtcg atgacgatgc agccggcggc
     5101 cgcggctttc ggcgcgtact gcttcgacac gtcgccgccc gccgagaaca ggccgatctg
     5161 cgcacgcgag aaatcgaacg tggcgacatc gatgaccgga atgtgccggc cgcgaaattc
     5221 cacgcgcttg ccgaccgagc ggctgctggc gagcggcagc agctcgctca ccgggaagtc
     5281 acgctcttcg agtatgcgga tcatcatgtc gccgacgagt cccgtggcgc cgagcacgca
     5341 gacgcgataa cccttcggcg gatctttctt cacttcgttc atgagactat ctaccttgtc
     5401 cggtgatcac gggcgtatcg cagcgcacgt cggcgttctg tccgcgatgc cgcagatagt
     5461 gatccatgag cacgatggcg agcatggcct cgcagatggg cgtggcgcgc agacccacgc
     5521 aagggtcgtg ccggccggtc gtcaccacgt ccaccgcgtt gccggcgacg tcgatggtct
     5581 tgcccggaac ctggatgctc gaagtgggct tgaacgtgac gtgcacgagc acgtcctgcc
     5641 cggaggaaat gccaccaaga attccgccgg cgttgttgct caggaatccc gtcggcgtga
     5701 gttcatcgcg ctgcacgctg cccatctgcg cagcggaagc cacacccgca ccaatctcca
     5761 cggcttttac ggcattgatg cccatcatgg cgtgcgcgag gtcggcgtcg aggcggtcga
     5821 acaccggctc accgagcccc ggcgggacgc cgcgcgcgag caccgtgacc tttgcgccga
     5881 gtgagtcgcc atcgccgcgg atattccaca tgaggtcttc gagttc
//
LOCUS       DATDEU010000160         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_173118, whole genome shotgun sequence.
ACCESSION   DATDEU010000160 DATDEU010000000
VERSION     DATDEU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173118"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2378)
                     /locus_tag="VIV63_04015"
     CDS             complement(<1..2378)
                     /locus_tag="VIV63_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123792.1"
                     /translation="MTRSKLRKIRRNQSKQARALLTGFPLAGALFIGAPVVHAQQVEE
                     PSGGLEEVTVTALKTQQNLQDVPVSIQAIGTERLEELNITSFDDYVKYLPSVSYQSAG
                     PGFARVFMRGAAAGDNGNHSGPQPGVGQYLDEQPITTIQGALDIHMYDIARVEALAGP
                     QGTLYGASSQSGTIRIITNKPDPSGFDAAYSIQVSDFAHGDLGYLGEGFTNIPLSDNA
                     AIRLVGWVRHDGGYIDNVEATRTFPTSGISMNSLANADYNDADTFGARAALKIDLNDT
                     WSITPSLMYQKQKGNGIYAGEGGFGPEADLGDLEVAHWHPENSNDEWAQAALTVEGKF
                     SNFDITFASSYLKRDVDVNSDYSDYAFFYDTIAGYGTYFYDNAGDLINPSQFINGKDG
                     YTKTSNELRFSTSAENRWRFVGGLFAQRQTHDIQQEYKITGLNDDSDVTGWDDTFWLT
                     KQWRVDRDMAVYGEVTFKATDRLEFTGGARYFRTENSLEGFFGFGATNPYGSSTGEAS
                     CFDTGHVFETAPCTNLDRTVEQNDTTFRFNTTFHATDDVMFYATWSEGFRPGGVNRRG
                     TFPPYKADFLTNYEIGWKTAWADNRLRFNGAIFKGDWDDFQFSFLGENGLTNISNAGN
                     AQLEGIEMDVQWAATDAFTLYGGLAFQKAELGEDFCKTLDANGVPLNEADCLANDPAD
                     FAPEGTRLPTTPEFKASLTGRYEFDMNGLDAHFQASVVHNGDAATALLPAENDVLGDS
                     EAYTIVDFSFGFEREKWSAELFIDNVFDERASLYRYSECDVAICSNPLEAGIV"
     gene            complement(2429..>2714)
                     /locus_tag="VIV63_04020"
     CDS             complement(2429..>2714)
                     /locus_tag="VIV63_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY6123793.1"
                     /translation="VLPGRVHRVLYENMVEDTEAEVRRLLDYCGLPFEEAVLRFHENQ
                     RAVRTASSEQVRQPIFKEGVDHWRHFEPWLGPLEEALGDVVPAYPATPQF"
BASE COUNT          463 a          806 c          891 g          554 t
ORIGIN      
        1 acgattccgg cttccagcgg attggaacag atcgcgacat cgcactccga gtagcgatac
       61 aggctcgcgc gctcgtcgaa cacgttgtcg atgaacagct ccgccgacca cttctcgcgt
      121 tcgaagccga acgagaagtc gacgatcgtg taggcctccg aatcgccgag cacgtcgttc
      181 tctgccggca gcagcgccgt cgcggcatcc ccgttgtgca cgacggaagc ctggaagtgc
      241 gcatcgagtc cgttcatgtc gaactcgtat cgccccgtga gactggcttt gaattccggc
      301 gtcgtcggca ggcgtgtgcc ttccggcgcg aagtccgcgg ggtcgttggc caggcaatcc
      361 gcctcgttga gcggaacgcc attggcgtcc agcgtcttgc agaagtcttc gccgagctcg
      421 gccttctgga acgcgaggcc gccatacaac gtgaacgcat ccgtggctgc ccattgcacg
      481 tccatttcga tgccttcgag ctgggcgttg ccggcattgg agatgtttgt caggccgttt
      541 tcgccgagga acgagaactg gaagtcgtcc cagtcgccct tgaagatggc gccgttgaag
      601 cgcagacgat tgtccgccca cgcggtcttc cagccgattt catagttggt gaggaagtcg
      661 gccttgtaag gagggaacgt accgcggcgg ttgacgccgc ctgggcggaa gccttccgac
      721 cacgtggcgt agaacatgac gtcatcggtc gcgtggaacg tggtgttgaa gcggaacgtg
      781 gtgtcgttct gctccacggt gcgatcgaga ttggtacacg gcgcggtttc gaagacgtgg
      841 ccggtgtcga agcaagatgc ttcgcccgtg ctcgatccat acggatttgt ggcgccgaac
      901 ccgaagaatc cttcgagcga gttctccgtg cggaaataac gcgcgccacc ggtgaactcc
      961 agccggtccg tggccttgaa cgtgacctcg ccgtaaacgg ccatgtcgcg gtccacgcgc
     1021 cactgcttcg tgagccagaa cgtatcgtcc cagcccgtca cgtcgctgtc gtcgttgaga
     1081 ccggtgatct tgtattcctg ctggatgtcg tgcgtctgac gctgcgcgaa caggccgccg
     1141 acgaaacgcc agcggttttc cgcggacgtc gagaagcgca gttcattgct ggtcttggtg
     1201 tagccgtcct tgccgttgat gaactgggac ggattgatga ggtcgccggc gttgtcgtag
     1261 aaatacgtgc cgtaacccgc gatcgtgtcg tagaagaacg cgtagtccga gtagtcggag
     1321 ttcacgtcga cgtcacgctt gagataggag ctcgcgaacg tgatgtcgaa gttgctgaac
     1381 ttgccttcga cggtcaacgc ggcctgcgcc cactcgtcat tggagttttc cgggtgccag
     1441 tgggcgactt cgagatcacc cagatcggct tcaggaccga atccgccttc accggcgtag
     1501 atgccattgc ccttctgctt ctggtacatc agcgagggcg tgatgctcca ggtgtcgttg
     1561 aggtcgatct tgagcgcggc gcgcgcgccg aaggtgtcgg cgtcgttgta gtcggcgttc
     1621 gccagcgaat tcatggagat gcccgacgtc gggaacgtgc gggtggcttc cacgttgtcg
     1681 atgtagccgc cgtcgtgacg aacccagccg acgagccgga tggcggcgtt gtcgctcaac
     1741 ggaatgttcg tgaaaccttc gccgaggtag ccgagatcgc catgggcgaa atcgctgacc
     1801 tggatgctgt aggccgcgtc gaaacccgac ggatcgggct tgttggtgat gatgcggatg
     1861 gtgcccgact gcgagctggc gccgtacagc gtgccctgcg gaccggccag cgcttcgaca
     1921 cgcgcgatgt cgtacatgtg gatatcgagc gcgccctgga tcgtggtgat gggctgctca
     1981 tcgagatact ggccgacacc cggctgcggg ccggagtggt tgccgttgtc gcccgccgcg
     2041 gcgccgcgca tgaacacgcg cgcaaagcct gggccggcgg actggtatga cacgctcggc
     2101 agatacttca cgtagtcgtc gaaactcgtg atgttgagct cttcgagacg ctcggtgccg
     2161 atggcctgga tgctgaccgg cacgtcctgc aggttctgct gggtcttgag cgcggtgacg
     2221 gtcacttctt cgaggccgcc cgacggctcc tccacctgtt gcgcgtgcac gacgggggcg
     2281 ccgatgaaca gggccccggc gagcgggaat ccagtcagca gagcgcgcgc ctgcttcgat
     2341 tgattacgcc ggatcttgcg cagtttgctg cgtgtcatga gtgatccccg tgttgttgtg
     2401 gctggagtgt atgccagccg ccacgaattc aaaactgcgg cgttgcggga tacgccggaa
     2461 cgacatcgcc gagcgcctcc tcgagcggcc cgagccaggg ctcgaagtgt cgccagtggt
     2521 ccacaccttc cttgaaaatg ggctggcgga cctgttccga gctcgcggta cgcacggcgc
     2581 gctgattttc atgaaatcgc agcacggcct cttcgaacgg caggccgcag tagtcgagca
     2641 accgccgtac ctcggcttcg gtgtcctcga ccatgttttc gtagagcacg cggtggaccc
     2701 ggccgggcag cacg
//
LOCUS       DATDEU010000161         4396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2070118, whole genome shotgun sequence.
ACCESSION   DATDEU010000161 DATDEU010000000
VERSION     DATDEU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070118"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VIV63_04025"
     CDS             complement(<1..809)
                     /locus_tag="VIV63_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEY6123794.1"
                     /translation="MEKNTEEEWQFSATGLDTARAWLAEQPHHASERRLVTKPTLTLT
                     DSYYDSPDWMIFRAGFALRLRHESADAGDARTEVTLKSLNAPRNGLAHRTEISQRFDG
                     ADMSDVIASAGIGERIRELIGERPLALLFTANTRRERRQLLEAGSDLALAEVDLDETS
                     IEAPSGASQQLKRVEVECINATPAAIEPFVAQLRDAAQLEPVSMSKFHSGLETAGLRP
                     GAELEFASGEITARQPFADTQFALLRRYFALVIDLEPLARSGSPTALHEMRV"
     gene            complement(927..2228)
                     /gene="purD"
                     /locus_tag="VIV63_04030"
     CDS             complement(927..2228)
                     /gene="purD"
                     /locus_tag="VIV63_04030"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY6123795.1"
                     /translation="MKILIVGSGGREHALAWKCAQSAKVSEVLVAPGNGGTATEPRVR
                     NVNVAAEDVAGLVALAKAENVGLTIIGPEGPLVVGVVDAFAAAGLRCFGPRKAAAQLE
                     GSKAFTKEFLKRHGIPTAAYATFTRETFDADWVRRQRTPIVVKASGLAAGKGVVIVDT
                     AEEAIATAQSMFGGQFGSAGDQVVIEEFLPGEEASFIVMADGKNIVTMATSQDHKRLK
                     DADAGPNTGGMGAYSPAPVVDRAMHERIMREVIEPSIRGLAKDGTPYTGFLYAGIMVH
                     PDGTPNVLEYNCRFGDPETQPIMMRLKSDLVELCEAALDHRLDQVHADWDSRAALGVV
                     LAAENYPDNVRKGDVIAGLDVAAKLPGKVFHAGTRAEDGKVLTQGGRVLCAVGLGDDV
                     SAAQTQAYGLVDVISWAGMQCRRDIGYRAIERERAAKPTRS"
     gene            complement(2240..3805)
                     /gene="purH"
                     /locus_tag="VIV63_04035"
     CDS             complement(2240..3805)
                     /gene="purH"
                     /locus_tag="VIV63_04035"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY6123796.1"
                     /translation="MLVTIRRALLSVSDKTGLVEFARALAARKVELLSTGGTARSLRD
                     AGLEVIDVSAYTGFPEIMDGRVKTLHPRVHGGLLGRRGVDEAVMAKHDIPPIDLLVVN
                     LYPFAQTVAKPDCTYADAIENIDIGGPAMVRAASKNHESVTVVVDPADYGQVLGELDA
                     NDGATSIDTRSSLAAKAFAHTARYDTMVSNYLLGRESDDAASFPATLPLVYEKIQDLR
                     YGENPHQKAAFYRELGLTGSCVAGATMLQGKELSFNNIADTDTAVECVRVFNEPACVI
                     VKHANPCGVATAVSLADAYDRAYRTDPTSAFGGIIAFNRELDAETAIAITGRQFVEVI
                     AAPSVSAEAAAVLAGKANVRVLVIGPLSKRTPTELEYRSVVGGLLVQSRDTALAQPDS
                     FKIVTSKQPTPTQYADLWFAWRVCKYVKSNAIVFARDGMTIGVGAGQMSRVYSTRVAA
                     LKAKDEGLQVEGSSMASDAFFPFRDGIDVAAEYGIKAIVQPGGSKRDEEVIAAANEHG
                     MTMVFTGMRHFRH"
     gene            complement(3819..4103)
                     /gene="fis"
                     /locus_tag="VIV63_04040"
     CDS             complement(3819..4103)
                     /gene="fis"
                     /locus_tag="VIV63_04040"
                     /inference="COORDINATES: protein motif:HMM:NF001659.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HEY6123797.1"
                     /translation="MTAKTSRTRKDSGRNGALEVPLRNHAERALSDYFSNLNGHKPAH
                     LYDLVLREVEEPLFRAVLDYSSGNQCRAAAILGINRGTLRKKLRQFGLSG"
     gene            complement(4146..>4396)
                     /locus_tag="VIV63_04045"
     CDS             complement(4146..>4396)
                     /locus_tag="VIV63_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEY6123798.1"
                     /translation="TILAHLESLYAFYGEETGLRVARKHLGWYFEKLQDAPDVHRELM
                     AASTSPSQFALTKQSLDLWMQTRVETTATTWGGIAACV"
BASE COUNT          707 a         1381 c         1477 g          831 t
ORIGIN      
        1 acccgcatct catgcagcgc ggtgggggaa ccggatcgcg ccagcggttc aagatcgatg
       61 accaacgcga agtatcggcg cagcagcgcg aactgagtgt cggcgaacgg ctgccgcgcg
      121 gtgatctcgc cgctcgcgaa ctcgagctcc gcgcccgggc gaaggcctgc cgtttccagc
      181 cccgagtgaa atttggacat cgacaccggt tcgagctgcg cggcgtcgcg aagttgcgcg
      241 acgaagggtt cgatcgccgc cggtgtcgca ttgatgcatt cgacttcgac gcgcttcagt
      301 tgttgtgagg cgcccgaggg ggcttcgatg gacgtttcgt cgaggtccac ctccgcgagc
      361 gcgagatcag agccggcttc cagcaattgc cggcgctcgc gccgcgtatt ggctgtgaac
      421 agcagcgcca gcggccgctc gccgatgagt tcgcgaatcc gttcgccgat gccggcgctg
      481 gcgatgacgt cgctcatatc cgcaccatcg aagcgttgcg agatctccgt gcgatgagcg
      541 aggccgttgc gaggggcgtt cagcgacttg agcgtcactt ctgttcgcgc atcacctgcg
      601 tcggcgcttt cgtgccgcag gcgcagcgcg aagccggcgc gaaagatcat ccagtcgggt
      661 gaatcgtaat aactgtccgt gagcgtcagc gtgggcttgg tcacgaggcg gcgctcggaa
      721 gcatggtgcg gttgctccgc aagccaggct cgcgctgtgt cgaggccggt ggccgagaat
      781 tgccattcct cttcggtgtt cttttccatg ttcttgtgag accaccgcgt cggccgcgaa
      841 ttcacaggcg aactcggcgg tcactgtatg cccggctggc ggatcgagtc catcggggtg
      901 cgagcccgcc gcgcggggct cgtggttcag gagcgggtcg gcttcgcggc ccgctctctc
      961 tcgatggcgc gatagccgat atcgcgccgg cactgcatgc cggcccagct gatcacgtcc
     1021 accagtccgt acgcctgggt ctgcgccgcg gacacgtcgt ccccgaggcc caccgcgcag
     1081 agcacgcggc cgccctgcgt gagcaccttg ccgtcttctg ccctggtgcc cgcgtggaac
     1141 acttttccgg gtagtttggc cgcgacatcg agcccggcga tgacgtcgcc cttgcgcacg
     1201 ttgtcggggt agttctcggc cgcaagcacc acgccgagcg cagcgcgtga gtcccagtcg
     1261 gcgtgcacct ggtcgagtcg gtgatccagc gccgcttcgc agagctccac gaggtcgctc
     1321 ttgagccgca tcatgatggg ctgggtttcc gggtcgccga atcggcagtt gtactcgagc
     1381 acgttgggcg tgccgtcggg gtgaaccatg atgccggcgt acaggaagcc cgtgtacggc
     1441 gtgccgtcct tcgccagtcc gcgaatactg ggctcgatga cttcgcgcat gatgcgttcg
     1501 tgcatcgcgc ggtcgaccac gggcgcgggc gaatacgcgc ccatgccgcc ggtgttcggt
     1561 ccggcgtccg cgtccttgag gcgcttgtgg tcctgcgacg tcgccatggt cacgatgttc
     1621 ttgccatcgg ccatgacgat gaagctcgct tcttcgcccg gcaggaattc ttcgatgacg
     1681 acctggtcgc cggcggagcc aaactgtccg ccgaacatcg actgcgcggt ggcaatggct
     1741 tcttcggcgg tatcgacgat gactacgcct ttgcccgccg ccaggccgct cgccttgacg
     1801 acgatgggcg tgcgctgccg gcgcacccag tcggcatcga acgtttcacg agtgaacgtg
     1861 gcgtaggccg cggtgggtat gccgtggcgt ttcaggaatt ccttggtgaa cgccttggag
     1921 ccttcgagtt gcgcggcggc cttgcggggc ccgaagcaac gcaagcctgc ggcggcgaac
     1981 gcgtcgacca cgccgaccac cagcggaccc tcggggccga tgatggtgag cccgacgttc
     2041 tcggccttgg ccagcgccac gagaccggcg acgtcctcgg cggcgacgtt gacgttgcgc
     2101 acgcgcggtt cggtggcggt gccgccgtta ccgggcgcga cgaggacttc ggaaaccttc
     2161 gccgattgcg cgcacttcca cgcgagcgca tgttcgcgcc cgccagaacc cacgatcagg
     2221 attttcatga cgcccgggat cagtgacgga aatggcgcat gccggtgaac accatggtca
     2281 tgccgtgttc attggccgcg gcgatgactt cctcgtcgcg tttgctgccg ccgggctgca
     2341 cgatggcctt gatgccgtat tcggcggcga cgtcgatgcc gtcgcggaac ggaaagaagg
     2401 cgtccgaggc catggagctg ccttcgacct gcagaccttc gtccttcgcc ttgagcgcgg
     2461 ccactcgcgt ggagtacacg cgcgacatct gcccggcgcc gacgccgatg gtcatgccgt
     2521 cgcgtgcgaa cacgatggcg ttggatttga cgtatttgca cacgcgccac gcgaaccaca
     2581 ggtccgcgta ctgagtcggc gtgggctgct tcgacgtgac gatcttgaag gagtccggtt
     2641 gcgccagcgc ggtatcgcgg ctctgcacga ggagtccgcc gaccacgctg cgatattcga
     2701 gctcggtggg cgtccgcttc gacaacgggc cgatcaccag cacgcgcaca ttcgccttgc
     2761 ccgcgagcac ggccgcggct tccgcggata ccgacggcgc tgcgatgact tcgacgaact
     2821 ggcggcccgt gatcgcgatg gccgtctcgg cgtcgagttc gcgattgaag gcgatgatgc
     2881 cgccaaacgc agaggtggga tcggtgcggt acgcgcggtc gtaggcgtcg gccagcgaca
     2941 cggcggtggc gacaccgcac gggttggcgt gtttgacgat gacgcaggcc ggttcgttga
     3001 acacgcgcac gcattccacg gcggtgtccg tgtcggcgat gttgttgaac gacagttcct
     3061 tgccctggag catcgtggca cccgccacgc aggaacccgt aagaccgagt tcacggtaga
     3121 acgccgcttt ctgatgcggg ttctcaccat aacgcaggtc ctgaattttc tcgtacacca
     3181 gcggcagggt tgccggaaac gacgcagcgt cgtcgctctc acggccgagc aaatagttgg
     3241 acaccatcgt gtcgtagcgc gcggtgtgcg cgaacgcttt cgcggcgagg ctcgagcgcg
     3301 tgtcgatgct cgtggcgccg tcgttggcgt cgagttcgcc gagcacctgg ccgtaatcgg
     3361 ccggatcgac gacgaccgtg accgattcat gatttttgga ggcggcgcgc accatggccg
     3421 gcccgccgat gtcgatgttt tcgatggcgt ccgcgtacgt acagtccggt ttggcgacgg
     3481 tctgcgcgaa tggatacaga ttcacgacca ggaggtcgat cggcgggatg tcgtgcttgg
     3541 ccatgacggc ttcgtcgacg ccacgacggc ccaggaggcc gccatgcacg cgcgggtgca
     3601 gcgtcttgac gcgcccgtcc atgatctcgg gaaagcccgt gtaggcgctg acgtcgatga
     3661 cctcgaggcc ggcgtcccga aggctgcggg cggtgccacc ggtgctcagc agctcgacct
     3721 ttcgggcggc cagggcgcgg gcgaattcaa cgaggcccgt cttgtcggag accgacagca
     3781 gggcgcggcg aatggtgacg agcatcagtg ctctttgatc agccggaaag accgaactgg
     3841 cgcagttttt tgcgcagcgt gccccggttg atgccgagga tcgcggcggc gcggcactgg
     3901 ttgcccgagg agtagtcgag caccgcgcgg aacagcggct cttcgacttc acgaagcacg
     3961 aggtcgtaga ggtgggccgg cttatgaccg ttcagattgg aaaagtaatc ggacagcgca
     4021 cgctcggcgt gattgcgcaa aggcacttcg agcgcaccat tgcgtccgga gtctttacgt
     4081 gtccgtgaag ttttcgccgt cattccgcgc cccatcgtcg tgtccgagcc tgaacacttt
     4141 ctaccttaga cacacgccgc gatacctccc caggtcgtgg cggtggtctc gactctggtc
     4201 tgcatccaga gatccagact ctgcttcgtc aacgcaaact gtgacgggga cgtcgaagct
     4261 gccatcaact cccgatgaac atcgggcgcg tcctgcaact tttcgaaata ccaaccgagg
     4321 tgtttgcgcg ctactcgaag tcctgtctct tcaccgtaaa aagcgtagag ggattcgaga
     4381 tgcgcgagga tggtag
//
LOCUS       DATDEU010000162         2791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_319122, whole genome shotgun sequence.
ACCESSION   DATDEU010000162 DATDEU010000000
VERSION     DATDEU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319122"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VIV63_04050"
     CDS             complement(<1..464)
                     /locus_tag="VIV63_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY6123799.1"
                     /translation="MPPKFQTHRFDLPPDFAGRRLDQALAQLLPQYSRTRIQRWIEDG
                     AVLINGLKPRVRDLVTGGEAATVEARLPEESGVEAEKLPLDIVHQDAAIIVINKPSGL
                     VVHPGAGNRAHTLQNALLAHDAKLKRVPRAGLVHRIDKDTSGLLVVARTLEA"
     gene            573..1373
                     /locus_tag="VIV63_04055"
     CDS             573..1373
                     /locus_tag="VIV63_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY6123800.1"
                     /translation="MRTAGSLAILFLALSLSLTGCRSKDGREGPMTDPAAVYERAHRA
                     LIQADYGLAIRIYEALMARYPFAAESRQARLDVIYAYYRAGESESAIDAADTFIEENP
                     THPRVDYAWYLKGLTDFERMPNFMERWFRVDLNQRPPTQARRSFLAFATVVEQYPNSE
                     YAHDARRRMVYLRNRLADYEIAVARYYVDRGAYVAAAQRAKVAIEEFDGAPAVRTALE
                     IMVHCYEQMELKELAAQTRAMYRNNYEGEVGERKGGTKKKWYKLWLAS"
     gene            1507..2184
                     /locus_tag="VIV63_04060"
     CDS             1507..2184
                     /locus_tag="VIV63_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY6123801.1"
                     /translation="MPRTARAIVANGCYHVLNRANRKAQVFHERADYEQFAALISRAQ
                     ARLTVPILAACLMPNHVHFVMRPVAAGDLANWMHWVFTTHVRWYHAKYSTTGRVWQGR
                     FKAFAVQEDHHLLTLMRYVERNAMRARMVERAEDWEWGSLAWRRARHAPVALAESPVP
                     LPSYWRQFANDPQTAVELAEIRECANRQRPFGASDWVARQVEELGMESSVTPVGRPPR
                     SRRVPFC"
     gene            complement(2204..>2791)
                     /gene="nadE"
                     /locus_tag="VIV63_04065"
     CDS             complement(2204..>2791)
                     /gene="nadE"
                     /locus_tag="VIV63_04065"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEY6123802.1"
                     /translation="IGEMMKATEATLVDEFAGRKPDTAEENIQSRCRMLLLMGISNKT
                     GKMLLTTGNKSEMAVGYATLYGDMAGGFAPIKDCSKLLVYRLAEYRNSVAAVIPRRVI
                     ERPPSAELRPDQKDSDSLPPYEILDPILEAFIEDDLSVDEICAKGFERATVVRVLDLV
                     KRNEYKRRQAPPGVRVSRRAFGRDWRYPITSGYRR"
BASE COUNT          492 a          897 c          895 g          507 t
ORIGIN      
        1 tgtgcctcga gcgtgcgtgc gacgactaac aacccgctgg tgtccttgtc gatgcggtgc
       61 acgagtccgg cgcgcggtac gcgcttgagc ttcgcgtcgt gcgcgagcag cgcgttctgc
      121 agggtatgcg cgcggttgcc ggcgcccggg tgcacgacga gcccggatgg cttgttgatg
      181 acgataatcg cggcgtcctg gtggacgatg tcgagcggca gcttttcggc ctcgacgccg
      241 ctctcctcgg gcagccgcgc ctcgaccgtg gcggcttcgc ccccggtgac gagatcgcgc
      301 acccggggct tgaggccgtt gatcagcacg gccccgtcct cgatccagcg ctggatgcgc
      361 gttcgcgagt actgcggcaa aagctgggcg agcgcctgat ccagccgcct gccggcgaaa
      421 tccgggggca gatcgaagcg atgggtctgg aacttggggg gcaacctgtt ggtccgatgg
      481 ctggcggggc cgctatactt cacgcgtccg gcgaccattt caaactatag ataccggcaa
      541 cccgagagac ggcaaaacct tccatgcgag caatgcgaac cgccggtagc ctggcaatcc
      601 tgtttttagc gcttagcctc agcctgaccg gctgccgatc caaggacggc cgcgaaggcc
      661 ccatgacgga cccggcggcg gtttacgagc gcgcccaccg cgccctcatc caggcggact
      721 atggcctggc catccggatc tacgaagcgc tcatggcccg gtacccgttc gcggccgagt
      781 cgcggcaggc gcgcctggac gtcatctacg cgtactaccg tgcgggcgag tccgagtcgg
      841 ccatcgacgc ggcggacacg ttcatcgaag agaacccgac ccacccgcgt gtcgattacg
      901 cgtggtattt gaagggcctt acggatttcg agcgtatgcc caacttcatg gagcgctggt
      961 tccgcgtgga cctgaaccag cgtccgccca cgcaggcgcg ccgctcgttc ctggcgtttg
     1021 cgaccgtggt cgagcagtac ccgaacagcg aatacgcgca cgatgcgcgc cgccgcatgg
     1081 tgtacctgcg caatcgcctg gcggactatg aaatcgccgt ggcgcgttac tacgtggatc
     1141 gcggcgccta tgtcgcggcc gcgcagcgcg cgaaagtggc catcgaagaa ttcgacggcg
     1201 cgcccgcggt gcgcacggcg ctcgagatca tggtgcattg ctacgagcag atggagctga
     1261 aggagctcgc cgcgcagacg cgcgccatgt atcgcaacaa ctacgaaggc gaagtcggcg
     1321 agcgcaaggg cggcacgaag aagaagtggt acaagctctg gctcgcgagt taatggggac
     1381 attcctgatt tgctagcaaa aggggacgcg gctcaaattc gctcccgaca ctagcttcga
     1441 gaaactcttg attcgcgtgt gctgtgcaga tcgtgcacgc ggccccgcag atgtacgttg
     1501 cgcctcatgc cgcgcactgc ccgcgccatc gtcgccaatg gctgctacca cgtcctgaac
     1561 cgcgccaatc ggaaggcgca ggtcttccat gaacgggcgg attatgagca attcgcagcg
     1621 cttatctcgc gcgcgcaggc acgtctgacg gttccgattc tcgcggcgtg tctgatgccc
     1681 aatcacgtcc acttcgtcat gcgtcccgtc gccgcaggtg atctcgcgaa ctggatgcac
     1741 tgggtgttca ccacgcacgt gcgttggtat cacgccaaat actcgacgac cgggcgtgtg
     1801 tggcaggggc ggttcaaagc cttcgccgtc caggaagatc accatctcct gacactcatg
     1861 cgctacgtcg agcgcaatgc gatgcgggcc aggatggtcg agcgcgctga agactgggag
     1921 tggggaagcc tcgcgtggcg cagggcccgg cacgcgccgg tggcgctcgc cgagtcgccg
     1981 gttcccctac cctcgtactg gcgacaattc gccaacgatc ctcaaactgc cgttgagctc
     2041 gcggagatcc gagaatgcgc gaatcgccaa cggcctttcg gcgcatcgga ctgggttgca
     2101 cggcaagtcg aagaactggg catggagtct tcagtgactc ccgtgggacg accgccgcga
     2161 tcgaggcgcg tccccttttg ctagcaaatc aggaatgtcc ccattaccgc cggtagccgg
     2221 aagtaatcgg ataccgccag tcgcggccga acgcacggcg cgacacgcgc actccgggcg
     2281 gagcctgccg ccgcttgtac tcattgcgct tcacgagatc gagcacgcgc acgacggtcg
     2341 cgcgctcgaa acccttcgcg cagatctcat ccaccgacaa atcgtcttcg atgaacgcct
     2401 cgagaatcgg atcgaggatt tcgtacggcg gcagtgagtc gctgtccttc tgatccggcc
     2461 gcagttccgc tgacggcggc cgctcgatca cgcggcgcgg aatcacggcc gcgacgctgt
     2521 tgcgatattc cgccagccga tacacgagca gcttgctgca atccttgatc ggcgcgaaac
     2581 caccggccat gtcgccgtac aacgtggcgt aacccacggc catctcgctc ttgttgccgg
     2641 tggtcagcag catcttcccg gtcttgttgg agatgcccat gagcagcagc atgcggcagc
     2701 gcgactggat gttttcttcg gcggtgtcgg gcttgcgtcc ggcgaattcg tcgacgagcg
     2761 tggcctcggt ggctttcatc atctcgccga t
//
LOCUS       DATDEU010000163         6584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_611470, whole genome shotgun sequence.
ACCESSION   DATDEU010000163 DATDEU010000000
VERSION     DATDEU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611470"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VIV63_04070"
     CDS             <1..1176
                     /locus_tag="VIV63_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEY6123803.1"
                     /translation="MPAKDFEKEAARLYSQVEPLYKGLHCYARKRLQQRYGKEKVPDG
                     KPIPAHLLGNMWAQQWNRVYDDLLKPYPAASIESADRLLQKQKWDAVKMTKSAENFYT
                     SIGFPTLPQTFWERSMLTRPRDREVVCHASAWDIDDKDDVRIKMCIKPIEEDLFTVYH
                     ELGHVYYYIWYKDQPILFQDGAHDGFHEAIGDAINLSVTPAYLRKIGLVGDVVPSKEA
                     IINQQMKMALDKIAFLPFGKLIDEWRWGVFDGRIKPAEYNKAWWELREKYQGVSAPLA
                     RSEEDFDPGAKYHIPGNTPYTRYFLSFILQFQFHKALCDASGYKGPLNECSIFGSTEA
                     GKRYGAMLAQGASKPWQDTLQELTGGREMDASAIIDYFKPLEAWLEEQNKGQSCGWD"
     gene            1188..3155
                     /locus_tag="VIV63_04075"
     CDS             1188..3155
                     /locus_tag="VIV63_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY6123804.1"
                     /translation="MNVNVAPAPQLTARAIILSIVLAVILAAANTYLGLFAGMTIASA
                     IPAAVVSMAVLRALGGGGILENNIVQTGASAATSIASGVIFTVPALVIMGYWTDFKYG
                     WVCAIAGLGGLLGVLFSVPLRRSLIVDQQMAFPEGKAAAEVLRAGENPKEGIKVLGVS
                     ALIGGIGKMIAASGMRLVPDSALASGFIGKYLTYMGTNISPALLGVGYIVGLNVGIVV
                     VAGSILSFNIAIPIYHTFFLADNPELAATVAGACAGIGSAECAEATAQILRGAQIRYL
                     GVGAMLVGGLWALITLRKSIVSGVKSGLAAARAGSDAIVAHTDRDLPMKWVLVGIVAF
                     TIPLAILYTSIVGSVGVGLAMSVIMIVTGFLFCSVSAYMAGLVGSSNNPMSGITIATI
                     LFAALVLLGFLGKESAIGPVAAVMIGAVVCCAACVAGDNLQDLKAGYLVGATPWRQQV
                     MLAIGSVCCALVMAPVLNMLAESYGIGPRTAEHPRSLEAAQANLMASVAKGLFGGHLP
                     WTMIMIGAGIGIAVIILDQILKSRGSKFRVPVLAAAIGIYLPLETMVPIFLGGLLNHL
                     VTRTFGKNLTEDEVEKRNRIGTLFAAGLITGEALIGIFIGFAIYGTKSADVFALPAGM
                     QLGGSVGEWAGLLVLGVVGYWLYRAGKRSPA"
     gene            3299..4258
                     /locus_tag="VIV63_04080"
     CDS             3299..4258
                     /locus_tag="VIV63_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY6123805.1"
                     /translation="MNPAERSHRTKLIVCFATVYFVWGSTYLVASIGVHALPPILFGG
                     IRFICAGILLGLVAIALGRKFELDATEWRHLFIVAIGSVLIPNACTNWAMQYVASNQT
                     AILNASAALWIAMFATRGRRAHALDRRTVIGLVIGFAGAALIVWPRGSLSASYFDMQL
                     LILIAVFVWSATTVYIRNVHSKLDLLSFSALQMLLGGLMMTGVGLAMGEAGDWTWSKP
                     GLIAMAYLTIASSCLAYTAFAWLAKNTTPAMVGTYSYVNPAIAAILGWWFLDEHLGVN
                     QLTGMAIMFAGVALVNWPQKDDKQGAKRLDEDEQKDPSTAGCG"
     gene            complement(4262..5044)
                     /locus_tag="VIV63_04085"
     CDS             complement(4262..5044)
                     /locus_tag="VIV63_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123806.1"
                     /translation="MRIALMRVHFSAVASFMLVATAALGGDRSFTGYARSLDNGELLY
                     VESHAVSGSGSGESRVVLYRCAEDSAPFARKQLAYSAKRTMPTFDFEDARSGFAEGFA
                     RDSSGLKVFERAGAGAPMRSELISATAELVVDAGFDEFVRANWDSLERGAVSRVPFLV
                     PSLLRSIDFRIRKAGETVIDGEAASVIRLSLAGALGWFTPDIDVSYRKRDRRLMRYRG
                     LTNIRDAGGELLKAQIDFPDAAPATTAVNLAAMRALPLAPCP"
     gene            complement(5126..5500)
                     /locus_tag="VIV63_04090"
     CDS             complement(5126..5500)
                     /locus_tag="VIV63_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY6123807.1"
                     /translation="MVGFILRGLVAALGLWAATEMLNGFTIDTPMTLILAGLLLGVVN
                     AIVRPFALLLSLPALIFTLGLFLLVVNAAMLGLVALVLPGFHIAGFWTAVGGAIIVSI
                     VSWIGSFFIGSRGRVEIIRAKE"
     gene            complement(5505..5966)
                     /locus_tag="VIV63_04095"
     CDS             complement(5505..5966)
                     /locus_tag="VIV63_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY6123808.1"
                     /translation="MSGQYRSFAHRVDVGAPVERVWRAFTDSRMLERWTTAGATVTPR
                     EKGRMQVVFGEGVELDAHIDVLVTPQRIRLILLPTRGMPGTDAVIVEDFLFERASPSA
                     TVVRLLSSGVPSNQAWVTYYLSKRRYWELSLTRLKVFLEKGLDQEQPKPRY"
     gene            complement(5963..>6584)
                     /locus_tag="VIV63_04100"
     CDS             complement(5963..>6584)
                     /locus_tag="VIV63_04100"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY6123809.1"
                     /translation="HWFVDVILHLDAHLVELLRDYGMWVYAILFAIVFAETGFVVTPF
                     LPGDSLLFAAGALAAVDTSGTLSAPLVSLTLIIAAVGGNTTNYHIGKWIGPPAFTGKI
                     RFLKVEYLQRTEAFFVKHGSMTIVLSRFVPIIRTCAPFVAGVARMPYARFQAYNVFGG
                     ITWVLLLVWAGFFFGNIPIIKENFGVVTIGIIVISLLPLAWVALKK"
BASE COUNT         1195 a         2071 c         2162 g         1156 t
ORIGIN      
        1 atgcccgcca aggatttcga gaaagaagcc gcgcgtctct acagccaggt ggagccgctc
       61 tacaagggcc tgcactgcta cgcgcgcaag cgcctgcagc agcgctacgg caaggagaag
      121 gtgcccgacg gcaagccgat ccccgcgcac ttgttaggca acatgtgggc gcagcagtgg
      181 aaccgcgtct acgacgatct gctgaagccg tatccggccg ccagcatcga atccgccgac
      241 cgcctgctgc agaagcagaa gtgggacgcc gtgaagatga cgaagtccgc cgagaacttc
      301 tacacgtcca tcggcttccc gacgctgccg cagactttct gggagcgctc gatgctcacg
      361 cgtccgcgtg accgcgaagt ggtttgccac gccagcgcct gggacatcga cgacaaggac
      421 gacgtgcgca tcaagatgtg catcaagccc atcgaggaag acctgttcac cgtctaccac
      481 gagctcggcc acgtctatta ctacatctgg tacaaggacc agccgatcct gttccaggac
      541 ggcgcgcacg acggcttcca cgaagccatc ggcgacgcca tcaacctctc ggtgacgccc
      601 gcctatctac gcaagatcgg cctggtcggc gacgtggttc cgtcgaagga agccatcatc
      661 aaccagcaga tgaagatggc gctcgacaag atcgcgttcc tgcctttcgg caagctcatc
      721 gacgaatggc gctggggcgt gttcgacggc cgcatcaagc ccgccgagta caacaaggcc
      781 tggtgggagc tgcgcgagaa ataccagggc gtgtccgcgc cgctggcgcg cagcgaagag
      841 gatttcgatc cgggcgcgaa atatcacatc ccgggcaaca cgccgtacac gcgttacttc
      901 ctgtcgttca tcctgcagtt ccagttccac aaggcgctgt gcgacgcgtc gggctacaag
      961 ggcccgctca acgagtgctc gatcttcgga agcaccgagg ccggcaagcg atacggcgcg
     1021 atgctcgcgc agggcgcatc caagccctgg caggacacgc ttcaggaact gaccggaggg
     1081 cgcgaaatgg atgcgtccgc catcatcgac tatttcaagc cgctggaagc atggctcgaa
     1141 gaacagaaca aaggtcaatc ctgcggctgg gattgaggag tagctagatg aacgtgaatg
     1201 tggcaccggc gccgcagctg actgcgcgcg ccatcatcct gtcgatcgtg ctcgcggtga
     1261 tcctcgccgc ggccaacacc tacctcgggc tgttcgccgg catgaccatc gcgtcggcga
     1321 ttcccgccgc ggtggtttcg atggccgtgc tgcgcgcgct cggcggcggc ggcatcctcg
     1381 agaacaacat cgtgcagacc ggggcgtcgg cggcgacatc gatcgcctcg ggcgtcatct
     1441 tcacggtacc ggcgctcgtg atcatggggt actggacgga cttcaagtac ggctgggtgt
     1501 gtgcgatcgc cggactcggc ggcctgctgg gcgtgttgtt ctcggtgccg ctgcgccgtt
     1561 cgctcatcgt cgatcagcaa atggcgtttc cagaaggcaa ggcggcggcc gaagtgctgc
     1621 gcgccggcga gaatccgaag gaaggcatca aggtgctggg cgtttcggcg ctcatcggcg
     1681 gaatcggcaa gatgatcgcc gcgagcggca tgcgcctggt gcccgacagc gcgctggcca
     1741 gcggcttcat cggcaaatac ctcacctaca tgggcaccaa catctctccc gccttgttag
     1801 gcgtgggcta catcgtcgga ctcaacgtgg gaatcgtggt ggtcgcgggc agcatcctgt
     1861 cgttcaacat cgcgatcccg atctatcaca cgtttttcct ggcggacaac cccgagctcg
     1921 cggccacggt cgcgggcgct tgcgccggga tcggcagcgc cgagtgcgcg gaagccacgg
     1981 cgcagatcct gcgtggcgcg cagatccgct atctcggagt cggcgccatg ctggtcggcg
     2041 gtctgtgggc cctgatcaca ttgcgcaagt cgatcgtgtc cggcgtcaag agcgggctcg
     2101 ccgcggcgcg cgccggcagc gatgcgatcg tggctcacac cgatcgcgat ctgcccatga
     2161 agtgggtgct ggtcggcatc gtcgcattca ccattccgct tgcgattctg tatacgagca
     2221 tcgtcggcag tgtcggcgtc ggcctcgcca tgtcggtcat catgatcgtc acgggcttcc
     2281 tgttctgctc ggtgtcggcc tacatggcgg gcctggtcgg ctcgtcgaac aacccgatgt
     2341 cgggcatcac catcgcgacc atcctgttcg ccgcgctggt gttgttaggg ttcctcggca
     2401 aggaatcggc gatcggcccg gtggccgcgg tcatgatcgg cgcggtggtc tgctgcgccg
     2461 cctgcgtggc gggcgacaac ctgcaggatc tcaaggccgg ttacctggtc ggtgcgacac
     2521 cctggcgtca gcaggtcatg ctggccatcg gctcggtgtg ctgcgcgctg gtgatggcgc
     2581 cggtgctcaa catgctcgcg gagagctacg gcatcggccc gcgcaccgcg gagcatccgc
     2641 ggtcgctcga agccgcgcag gccaatctca tggcatcggt cgccaagggt ttgttcggcg
     2701 gacatctgcc gtggaccatg atcatgatcg gcgcgggcat cggcatcgcc gtcatcatcc
     2761 tcgaccagat tctcaagtcg cgcggctcga agttccgcgt tcccgtgctc gccgcggcca
     2821 tcggcatcta cctgccgctc gaaaccatgg tgccgatctt cctgggcggt ctgctcaatc
     2881 acctcgtgac gcgcactttc ggcaagaacc tgactgaaga cgaggtggag aagcgcaatc
     2941 gcatcggcac gttgttcgcg gccggcctca tcaccggtga agcgctgatc ggcatcttca
     3001 tcgggttcgc gatctacggc acgaagagcg cggacgtgtt cgcgctgccg gcgggcatgc
     3061 agctcggcgg ttcggtcggc gaatgggccg ggctgctggt tctgggtgtg gtcggctact
     3121 ggctgtatcg cgccggcaaa cgcagcccgg cttgatggct taatggggac attcctgatt
     3181 tgctagcaaa tggggacggg gctcgtagag tcccgtccct ttatcttgcg gacatccgcg
     3241 aagcgcctga cgtacgatcc ggcgcaatcg ggcacgtcgc ccagttcatc tcgtcatcat
     3301 gaatcccgcg gaacgctcgc accgaacgaa gctcatcgtc tgcttcgcca ccgtctattt
     3361 cgtctggggc tcgacctacc tggtcgccag catcggcgta catgcgttgc cgcccatcct
     3421 gttcggtggc atccgcttca tctgcgcggg catcctgctc ggactcgtgg cgattgcact
     3481 gggccgcaag ttcgagctcg acgccaccga gtggcggcac ctcttcatcg tcgccatcgg
     3541 cagcgtgctg atccccaacg cctgcacgaa ctgggcgatg cagtacgtcg cgtccaatca
     3601 gaccgcgatc ctcaacgcgt cggcagcgtt gtggatcgcg atgttcgcca cgcgcgggcg
     3661 gcgtgcgcat gcgctggacc ggcgcacggt catcggcctc gtgatcggct tcgcaggcgc
     3721 ggcgctcatc gtctggccac gcggcagtct ttcggccagt tatttcgaca tgcagctcct
     3781 gatcctcatc gcggtgttcg tgtggtcggc gaccacggtg tacatccgca acgtgcattc
     3841 gaagctggat ctgttgtcat tctccgcgct gcagatgttg ttaggcggac tgatgatgac
     3901 cggcgtcggc ctggcgatgg gggaagcggg tgattggacg tggtcgaagc cggggctcat
     3961 cgcgatggcc tatctgacca tcgcgagctc ttgcctcgcc tacaccgcgt tcgcgtggct
     4021 cgcgaagaac accacgcctg cgatggtcgg aacgtacagc tatgtgaatc cggcgatcgc
     4081 ggcgattctg ggctggtggt tcctcgatga acatctcggc gtgaaccagc tcaccggcat
     4141 ggcgatcatg ttcgcgggcg tcgcgctcgt gaactggccg cagaaagacg acaaacaagg
     4201 tgcgaagcgg ctcgacgaag atgaacagaa ggatccgtcg acggctggct gtggttagcg
     4261 gtcacggaca gggtgcgagc ggcagtgcgc gcatcgccgc gagattcacc gcggtggtcg
     4321 cgggggcggc gtcggggaaa tcgatctgcg ccttcagcag ctcgccgccc gcgtcgcgga
     4381 tgttcgtgag gccgcggtat cgcatgaggc ggcgatcgcg tttccgataa ctgacatcga
     4441 tgtcgggcgt gaaccagccc agcgcgcctg cgagcgacag gcggatgacg ctcgcagcct
     4501 cgccgtcgat gacggtctcg ccggccttgc gaatgcggaa gtcgatactc cgcagcaggc
     4561 tcggcacgag gaaaggcacg cgcgagacgg cgccgcgctc caacgaatcc caattcgcgc
     4621 gcacgaattc gtcgaagccc gcgtcgacca cgagttcggc ggtggcagag atgagctcgc
     4681 tgcgcatcgg tgcgcccgca cccgcgcgtt cgaagacctt gaggccgctt gagtcgcgcg
     4741 cgaatccttc ggcgaatccg ctgcgcgcgt cttcgaaatc gaaggtgggc atggtgcgct
     4801 tcgcggaata cgcgagttgc ttgcgtgcga acggcgcgga gtcctcggcg catcggtaaa
     4861 gcaccacgcg cgattcaccg gagccggaac cgctgaccgc gtgggattcg acgtacagca
     4921 attcaccgtt gtcgaggctg cgggcataac cggtgaagga gcggtcaccg cccagggcgg
     4981 cggtcgcaac gagcatgaaa gacgcaacgg cggagaaatg gacgcgcatg agggcgatac
     5041 gcaacgcggc gcaggccgga tgcgtgaggt gcggcccctt ttgctaggaa atggggagtg
     5101 tccccatttc cttccagtgt ccccattact ccttggcgcg gatgatctcg acgcggccgc
     5161 gggagccgat gaagaaagag ccgatccagc tcacgatgct gacgatgatg gcgccgccga
     5221 cggcggtcca gaaaccggcg atgtgaaacc ccggcagtac cagcgcgacc agcccgagca
     5281 tggcggcgtt cacgacgagc aggaacaggc cgagcgtgaa gatgagcgcc ggcaacgaca
     5341 gcagcagcgc gaagggccgc acgatcgcat tcacgactcc gagcagcaga ccggccagga
     5401 tcagcgtcat cggagtgtcg atggtgaaac cgttcagcat ttccgtcgcg gcccagaggc
     5461 cgagcgcggc aacgaggccg cgcagaatga atcccaccat gacttcagta cctcggtttc
     5521 ggctgttcct ggtcgagacc cttttcgaga aacaccttga gccgggtcag cgacagctcc
     5581 cagtagcggc gcttggacaa atagtacgtc acccacgcct ggttcgaggg tactccactg
     5641 ctcagcagcc gcaccacggt cgccgacggg gaggcacgct cgaacaggaa gtcttcgacg
     5701 atcacggcgt ccgtaccagg catgccgcgg gtgggcagca ggatcagccg gatgcgctgc
     5761 ggcgtcacca gcacgtcgat gtgcgcatcg agctccaccc cttcgccgaa caccacctgc
     5821 atgcggccct tctcgcgggg tgtgacggtt gcgccggcgg tcgtccagcg ctcgagcatc
     5881 cggctgtcgg tgaaggcacg ccacacgcgt tcgaccggcg cgccgacgtc gacacgatgg
     5941 gcgaagctgc ggtactggcc gctcacttct tcagcgccac ccacgccagt ggcaacaggg
     6001 agatgacgat gatcccgatg gtcacgacgc cgaaattctc tttgatgatg gggatgttgc
     6061 cgaagaagaa acccgcccag accagcagca gcacccaggt gatgccgccg aacacgttat
     6121 acgcctggaa gcgcgcgtac ggcatgcggg cgactccggc cacgaagggg gcgcaggtgc
     6181 ggatgatggg cacgaaacgc gacagcacga tggtcatcga cccgtgtttc acgaagaacg
     6241 cctcggtgcg ttgcaggtat tccaccttga ggaaacggat cttccccgtg aaagccggcg
     6301 gccctatcca tttgcctatg tggtagttag tggtgttgcc gccgaccgcg gcgatgatga
     6361 gcgtcagcga caccagcggg gcggacaacg taccgctggt atctaccgcc gccagtgcgc
     6421 ccgcggcgaa cagcagcgag tcgcccggca ggaatggcgt gaccacgaag ccggtctcgg
     6481 cgaacacgat ggcgaagagg atggcgtaca cccacatgcc gtagtcgcgc agcagctcga
     6541 ccaggtgggc gtcgaggtgg aggatgacgt cgacgaacca gtgg
//
LOCUS       DATDEU010000164         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1632553, whole genome shotgun sequence.
ACCESSION   DATDEU010000164 DATDEU010000000
VERSION     DATDEU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632553"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..334
                     /locus_tag="VIV63_04105"
     CDS             104..334
                     /locus_tag="VIV63_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY6123810.1"
                     /translation="MSEIEDQDNVDRELFKNVEKLRELRIAHRDLDEVISRLSLDFQT
                     DQLQLKRLKKRKLILKDQITRLESELIPDLNA"
     gene            331..1605
                     /locus_tag="VIV63_04110"
     CDS             331..1605
                     /locus_tag="VIV63_04110"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY6123811.1"
                     /translation="MKKLFEQLTTPEVMAEALALLMAALLATALAHYIKNWLRPMATR
                     DTLGRWPKRFVVAGMVLAPSLLGVLLILGLRFLFASFDLETALIDVAMDLATVLVLVR
                     FALHALSVSLGPNSWIRVWELRLTFALWAAISFQVLGWFNGIERTFDSIDLIPGKTTF
                     SLWALLKSIVVIVGFLVATSLIARTIERRVMKLEGLAISTRIGISKFSTFMLVGLGIL
                     LGINASGVDLTVLGLFTGAIGLGLGFGLQAIASNFVSGFVLLLDKSIKPGDVISFTGT
                     TGTSTENFGWVQELRGRYIVVRDRDGVETLVPNQNLITNSVINWSYSDQRVRIRLPVM
                     ISFQDDPEVALQVLIDAAANHPRILKDPGPVSRLMSFENYGMRVEVRFWIRDPMNGVN
                     NVRSDVNREIWRLFKKHGITIPVQQHEVRVVQ"
     gene            1605..2030
                     /gene="arfB"
                     /locus_tag="VIV63_04115"
     CDS             1605..2030
                     /gene="arfB"
                     /locus_tag="VIV63_04115"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY6123812.1"
                     /translation="MSLLVRPGVEIPDTDIEMSAIRSAGPGGQNVNKVATAVQLRFSV
                     ARNTTLREDVKTRLRALAGQRMTDADDLLIVARSHRSQEQNRREAEERLCDLVRRALI
                     VPKKRHATKPTRASKERRLDGKARSQRTKRMRGKIKFDD"
     gene            2043..2798
                     /gene="tfpZ"
                     /locus_tag="VIV63_04120"
     CDS             2043..2798
                     /gene="tfpZ"
                     /locus_tag="VIV63_04120"
                     /inference="COORDINATES: protein motif:HMM:NF041437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfpX/TfpZ family type IV pilin accessory
                     protein"
                     /protein_id="HEY6123813.1"
                     /translation="MIVWREKLLATAIHFGVTLLLAGIAAALIFLVWFPDPFQTMIGG
                     TELFLLVVGCDLALGPLISLVIYNSRKSRRELVTDYSIVGAVQIAALVYGVFILAGTR
                     PVYVAYSGDRFEIVTAREITETELAAARDPQYRSLPLTGPRYIGIQVPQQDHNDAVFQ
                     ALAGNEEHMRPKFYVSYESQLPKIHARAKKLEELLQKKPASTPLLDAAMHEVSIPAER
                     VRWLPVHHRKGFWTALIDIDDGKPVAYFDFDPY"
BASE COUNT          529 a          928 c          846 g          501 t
ORIGIN      
        1 ggatttccac gatccggccg tgccgtggaa tccccttggc cggtggctta agcttccgag
       61 cggtctcgag ccggtcatga atcgccttac cgaaccttag ccgatgagcg aaatcgaaga
      121 tcaggacaac gtcgatcgcg agcttttcaa gaacgtggaa aagctgcgcg agctgcgcat
      181 agcgcaccgc gatctcgacg aggtcatctc gcggctgtcg ctcgacttcc agaccgatca
      241 gctgcaactc aagcggctga aaaagcgcaa gctgatcctc aaggaccaga tcacgcggct
      301 cgaaagtgag ctcataccgg acctgaacgc ttgaagaagc tcttcgagca gctcaccact
      361 cccgaagtca tggccgaggc gctcgcgctt ctgatggcgg cgctgctggc gaccgcgctc
      421 gcgcactaca tcaagaactg gctgcggccg atggccacgc gcgacacgct gggacgctgg
      481 ccgaagcgtt tcgtggtcgc gggcatggtg ctcgcgccgt cgctgctcgg cgtgctgctc
      541 atcctcgggt tgcgcttcct gttcgccagt ttcgacctcg aaaccgcgct catcgacgtc
      601 gccatggacc tggccaccgt gctggttctc gtacgcttcg cgctccacgc cttgagcgtg
      661 tcactcggac cgaatagctg gatccgcgtg tgggaactgc gcctcacctt cgcgttgtgg
      721 gcggcgatct ccttccaggt gctcggctgg ttcaacggca tcgaacgcac gttcgacagc
      781 atcgacctca tcccgggcaa gaccacgttc agtctctggg cactgctcaa gagcatcgtc
      841 gtcatcgtcg gcttcctggt cgccacgagc ctgatcgcac gcaccatcga acggcgcgtc
      901 atgaagctcg agggcctggc gatctcgacg cgtatcggca tctccaagtt ctccacgttc
      961 atgctggtcg gcctcggcat cctgctgggc atcaacgcat cgggcgtgga cctcacggtg
     1021 ctcggcctgt tcaccggcgc catcggttta ggcctggggt tcggcctgca ggccatcgcc
     1081 agcaacttcg tctccggctt cgtgctgctg ctcgacaagt ccatcaagcc gggcgacgtc
     1141 atcagtttca cgggcaccac cggcaccagc accgagaact tcggctgggt ccaggaactg
     1201 cgcggccgat acatcgtcgt gcgcgaccgg gacggcgtcg agacgctggt gcccaaccag
     1261 aacctcatca ccaactcggt gatcaactgg agctactccg accagcgcgt gcgtatccgc
     1321 ctgccggtca tgatcagctt ccaggacgac ccggaagtcg cgctgcaggt gctcatcgac
     1381 gccgccgcga accatccgcg catcctcaag gatccgggcc ccgtctcgcg gctcatgagc
     1441 ttcgagaatt acggcatgcg cgtcgaagtg cgcttctgga tccgcgaccc catgaacggc
     1501 gtcaacaatg tgcgttcgga cgtcaaccgc gagatctggc ggctgttcaa gaaacacggc
     1561 atcacgatcc ccgtgcaaca gcacgaagtg cgcgtcgtcc agtaatgagt ctgctcgtga
     1621 gaccgggcgt cgagattccc gacaccgata tcgaaatgtc ggccatacgc tccgccggtc
     1681 ccggcgggca gaacgtcaac aaggtggcca cggccgtcca gctgcgtttc tcggtcgcgc
     1741 gcaacaccac gctgcgcgag gacgtgaaga cgcggctgcg agcgttggcc ggccagcgca
     1801 tgaccgacgc cgacgatctg ctgatcgtgg cgcgctcgca tcgcagccag gagcagaacc
     1861 ggcgcgaagc cgaagaacgc ctctgcgacc tggtgcggcg cgcgctgatc gtgcccaaga
     1921 agcgtcacgc caccaaaccc acgcgcgcct cgaaagaacg gcgcctcgat ggcaaggcac
     1981 gttcgcagcg gaccaagcgc atgcgcggca agatcaagtt cgacgactga cggagaagcc
     2041 ccgtgattgt gtggcgtgaa aaactcctgg cgactgcgat ccacttcggc gtgacgttgt
     2101 tgctggccgg aattgcggca gcgctcatct tcctggtgtg gttccccgat cccttccaga
     2161 ccatgatcgg tggaacggag ctgttcctgc tggtggtggg ctgcgacctc gcgttgggcc
     2221 cgctgatctc gctggtgatc tacaacagcc gcaaatcacg ccgcgaactc gtgaccgact
     2281 actccatcgt cggcgccgtg cagatcgcag cgctggtcta tggagtcttc atcctggccg
     2341 gcacgcgccc ggtgtacgtg gcgtacagcg gagaccgatt tgaaatcgtc acggcgcgcg
     2401 aaatcacgga aaccgaactc gccgcggctc gcgacccgca ataccgctcg ctgccgctca
     2461 ccggtccgcg ttacatcggc atccaggtgc cgcagcaaga ccacaacgat gcggtattcc
     2521 aggcactcgc gggcaacgaa gagcacatgc ggccgaagtt ctacgtgagc tatgaatcgc
     2581 agctcccgaa gatccacgcg cgcgccaaga agctcgagga gctgctgcag aaaaagccgg
     2641 ccagcacacc gctgctggac gccgcgatgc acgaggtttc cattccggcc gaacgcgtac
     2701 gctggctgcc ggttcatcac cgcaaggggt tctggacggc gttgatcgac atcgacgacg
     2761 gcaaacccgt cgcgtatttc gacttcgatc cgtactgacc ggga
//
LOCUS       DATDEU010000165         2181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_903236, whole genome shotgun sequence.
ACCESSION   DATDEU010000165 DATDEU010000000
VERSION     DATDEU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903236"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2181
                     /locus_tag="VIV63_04125"
     CDS             <1..>2181
                     /locus_tag="VIV63_04125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6123814.1"
                     /translation="LPHAHVLSSALREHFTRERDILAGLSDARIARFYDAGVTEAGQP
                     WIALEYVEGTPLAEYCEKHALTTRARVALVREVAAAVQAAHARLVVHRDLKPANVMAT
                     GGGHIKLLDFGIASLLDDSADGGVTQAGASAASPDYAAPEQLRGGTITVATDVFGLGV
                     MAYELLAGQRPFPPRSRLGRMLDERGEAPLASTRVEGRRRGELAGDLDAILARAMETE
                     PERRYASVEAFAEDLTRHLAHEPVQARHLNRWRRCLKFMRRHRRGAGVAAALVLALAA
                     GVTGVLWQGQRAAEEARRANAIRDFLVETFRASDPRIASDQPRGTITARALLDAGAAR
                     IESRFADDPAVQIELLRTVADLYAQLGEDERYEALQALQVRKVREHYGPLHRNILDSA
                     VESAQRACTRGDRERCSTLVVAADELLKKAGDDDPERRAQWWLARGWQLRSEDGQIDA
                     GGQAFANAVGLYREHLPLTRGHVTALHELANFETAMKLDHARAITTFQQALSLALSLP
                     DRNDAELQTLYGNLGIVYQQTADFRAAADAFQRSAEIAERTTGAGFPTAWAPRVQAAR
                     TLHLAGERDAAHREFSRLVPLLPTDGKYAFDVANVREVYGERLANEGRPGLGIPHLEA
                     ALAFHAKQGLFSFKARLVRRYLGEAYSRAGRHADAGRMLEASLDEYLSQQPDGQQPVM
                     AIRESWGRWLLVDGKPDEAAMQFEAIIAAAGDRALAHVAMAHAG"
BASE COUNT          359 a          730 c          749 g          343 t
ORIGIN      
        1 aattgcccca cgcgcacgta ctctcgagcg cgctgcgcga gcacttcacg cgggagcgcg
       61 acattctcgc cggcctgtcc gatgcgcgca tcgcgcggtt ctatgacgcg ggtgtgacgg
      121 aagccggaca gccgtggatc gcgctcgaat acgtcgaggg cacgccgctt gcggagtact
      181 gcgaaaaaca tgcactcacg acgcgcgctc gcgtggcgct ggtgcgcgag gtcgccgccg
      241 cggtgcaggc cgcgcatgcg cggctggtcg tgcatcgcga tctcaaaccc gccaacgtca
      301 tggcaaccgg cggtggccat atcaaactac tcgatttcgg cattgccagc ctgctcgacg
      361 attccgcgga tggcggtgtc acgcaggcgg gcgccagcgc tgcgtcgccg gactacgcgg
      421 ctcccgagca gctccgcggc ggaacgatca ccgtcgcgac cgacgttttc gggctcggcg
      481 tgatggccta cgaattgctc gcgggccagc gcccgtttcc gccgcgctcg cgcctcggcc
      541 ggatgcttga cgagcgtggc gaggcaccgc tcgcctcgac gcgtgtcgaa gggcggcggc
      601 gcggcgaact cgccggggat ctcgatgcca tccttgcgcg agcgatggaa acagagccgg
      661 agcggcgcta cgcgtccgtc gaggcgttcg ccgaagatct cactcgccac ctcgcgcacg
      721 agcccgtgca ggcgcggcac ctcaatcgct ggcggcgatg cctcaaattc atgcgacgac
      781 atcgccgcgg cgcaggcgtg gctgccgcgt tggtgctggc gctggcggca ggggtgaccg
      841 gcgtgttgtg gcagggacag cgcgcggccg aggaggcccg ccgtgcgaat gcgattcgcg
      901 atttcctcgt ggagacgttc cgtgcgagcg atccgcgcat cgcctcggat cagccacgcg
      961 gcaccattac cgcgcgcgcg ttgctggacg ccggcgccgc gcgcatcgag tcgcgcttcg
     1021 cagacgatcc cgccgtgcag atcgagctgc tgcgcacggt cgcggatctt tacgcgcagc
     1081 tgggggaaga cgaacgatac gaagcgctgc aggcactgca agtgcgcaag gtccgcgaac
     1141 attacgggcc actgcatcgg aatatcctcg acagcgccgt ggagtcggct cagcgcgcct
     1201 gcacgcgcgg tgaccgtgag cgctgctcga ctctggttgt ggcggccgac gaactactga
     1261 aaaaagccgg tgacgacgat cccgaacgac gcgcgcaatg gtggctggcg cgcggctggc
     1321 aactgcgaag cgaagacgga cagatcgacg ccggcggcca ggcgttcgcc aacgcggtgg
     1381 gtctttatcg cgaacacctg cccctgacgc gtggacacgt cacggcgctg cacgagctcg
     1441 cgaattttga gacggcgatg aaactcgacc acgcccgggc catcacgacc tttcagcagg
     1501 cgctttcgct cgccttgtcg ctcccggatc ggaacgacgc ggaattgcag acgctgtacg
     1561 gcaatctcgg catcgtctat caacaaaccg ccgacttccg cgccgcggcg gacgcgttcc
     1621 aacgctcggc cgagatcgcc gagcgcacca cgggcgccgg tttcccgact gcctgggcgc
     1681 cgcgcgtcca ggccgcgcgc accttgcatc tcgctggcga acgcgacgcg gcgcaccgtg
     1741 aattctcgcg gctcgttccc ttgttgccga ccgacggcaa gtacgcgttc gacgttgcca
     1801 atgtgcgtga agtgtacggc gaacggctcg cgaacgaggg ccgccccggg ctcgggatcc
     1861 cgcatctgga agccgcgctg gccttccatg ccaaacaagg tttgttttcg ttcaaggcgc
     1921 gactcgtgcg ccgctacctc ggtgaggcgt attcgcgagc cggccggcac gcggacgccg
     1981 gccgaatgct cgaggcgtcg ctagacgaat atctctcgca gcaaccggat gggcagcaac
     2041 ccgtaatggc gattcgcgag agctggggtc gctggctgct cgtcgatggc aagccggacg
     2101 aagctgcgat gcaattcgaa gcgatcatcg cggcggcggg agatcgggca ctggcgcacg
     2161 tcgcgatggc gcacgcgggt c
//
LOCUS       DATDEU010000166         2222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1778608, whole genome shotgun sequence.
ACCESSION   DATDEU010000166 DATDEU010000000
VERSION     DATDEU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778608"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VIV63_04130"
     CDS             complement(<1..828)
                     /locus_tag="VIV63_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY6123815.1"
                     /translation="MKAIRTDDIAASYRPLAGVADELKTADGSIRPVWRPFHDYLARL
                     PADELAQRFARGDSYLRDAGVYFRQYGQRGSIDRAWPLSHIPVLLHEDEWSQIASGLS
                     QRADLLELVAADLYGPGNLAANGFLPAAAIAGSAEWLRPMVGTQPASGHFLHFVAFDI
                     GRGPSGEWWVLEDRVQAPSGAGFALENRVATARVFSDYYAEAHVPRVAGFFRKFRESL
                     GGIRGADDNGIAILSPGPMNDTYYEHAYLARYLGLALLEGEDLAVEKGRVVVRSISGT
                     "
     gene            complement(846..>2222)
                     /locus_tag="VIV63_04135"
     CDS             complement(846..>2222)
                     /locus_tag="VIV63_04135"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY6123816.1"
                     /translation="QLEGYAPPHDPRVNVIRVAPDPGVIEVNIHPASSWRECVATTET
                     VYEEARQCRLGADKFMIDGRHTGTGGGNHVVVGGQTPGDSPFLRRPDLLRSLILHWQR
                     HPSLSYLFSGLFIGPTSQAPRIDEARHDGLYELETALEQIPAQGAAPLPWLVDRLLRN
                     LLVDVTGNTHRSEICIDKLFSPDGPTGRLGLVEFRGFEMPPNPRMSLAQQVLIRALIA
                     RMWQSPLTGKLTRWGTSLHDRFMLPHFLWRDFLEVLDDLRLHGFALRPEWYEAQAEFR
                     FPYCGEVEFDSVGLELRQALEPWHVLGETGAIGGTVRYTDSSVERLQVKLTAPDPARY
                     AVTCNRRRVPLQQTESSSVRVAGVRFKAWQPAMALHPVLPVNAPLTFDIYDNWSGRAV
                     GGCIYHVAHPGGRSYDTFPVNANEAEARRLARFVRSGHSGGQYEARVEVPSPEFPMTL
                     DLRRPEGL"
BASE COUNT          397 a          741 c          731 g          353 t
ORIGIN      
        1 cgtaccggaa atgctgcgca ccacgacgcg ccccttctcc accgcgaggt cctccccctc
       61 cagcagagcg agccccaaat agcgcgcgag ataggcatgc tcgtaatagg tgtcgttcat
      121 cggtcccggc gagaggatcg cgatgccatt gtcgtcggcg ccccgaatgc cgcccagcga
      181 ctcgcggaac ttgcgaaaaa aaccggccac gcgcggtaca tgcgcctccg catagtagtc
      241 ggagaacaca cgggcggtgg cgacacggtt ttcaagcgcg aagccggcgc cggatggagc
      301 ctgcacgcga tcctccagca cccaccattc gccgcttggg ccgcgcccga tgtcgaacgc
      361 gacgaaatgc aggaaatgcc cggatgccgg ctgcgtgccg accatcgggc ggagccactc
      421 agcgctcccc gcaatcgccg cggcgggaag gaatccgttc gcggcaagat tgcccggccc
      481 ataaagatcg gcggcgacga gttccaacag atcggcgcgc tggctgagac cggaagcgat
      541 ctggctccac tcatcctcgt gcaacagcac ggggatgtgg ctcagcggcc aggcccggtc
      601 gatcgagccg cgttgaccgt attggcggaa atagacgcct gcgtcgcgca gataggaatc
      661 gccgcgcgcg aaacgctgcg cgagttcatc ggcgggaaga cgcgccagat agtcgtggaa
      721 cggtcgccag acgggccgga tcgaaccatc tgccgtcttc aactcatccg ctacaccggc
      781 aagcggccga taactggccg cgatgtcgtc cgtcctgatt gctttcaaac cgggcgcccc
      841 cgccactaca gcccctccgg cctgcgcaga tcgagcgtca tcgggaattc cggcgacgga
      901 acttccacac gcgcctcata ctgcccgccg ctatgccccg agcgcacgaa gcgcgcaagc
      961 ctgcgcgcct cggcttcgtt cgcgttcacc ggaaaggtgt cgtagctgcg cccgccggga
     1021 tgcgcgacgt gataaatgca tccacccacc gcgcggccgc tccaattgtc gtagatgtcg
     1081 aaggtcagcg gcgcgttgac cggcagaacc gggtggagcg ccattgcagg ctgccaggcc
     1141 ttgaagcgga cgccggctac acgcacgctg ctggattcgg tctgctgcag cggcacgcgg
     1201 cgccggttgc aggtgacagc gtagcgggca gggtcgggcg cggtcagctt gacctgcagc
     1261 ctttccaccg agctgtccgt gtagcgcacg gtgccgccga ttgccccggt ttcgccaagc
     1321 acgtgccagg gctccagcgc ctgccgcaat tccagcccga cgctgtcgaa ctccacttca
     1381 ccgcaatagg ggaagcggaa ctcggcctgc gcctcatacc attccggccg cagcgcgaag
     1441 ccgtgcagcc tgagatcgtc cagaacctcc aggaaatcgc gccatagaaa gtgcggcagc
     1501 atgaagcggt catgcaggct cgtgccccaa cgtgtcagct tgcctgtgag aggcgactgc
     1561 cacatgcggg cgatgagcgc gcggatcagc acctgctggg ccaggctcat gcgcgggttt
     1621 ggcggcattt cgaagccgcg aaactcgacc aggcccagcc gcccggtggg gccgtccggc
     1681 gagaacagct tatcgatgca gatttcggag cggtgggtat tgcccgtcac gtcgaccagc
     1741 aggttccgta gcagcctgtc tacaagccag ggcaacggcg ctgcgccttg cgcaggtatc
     1801 tgctcaagcg cggtttccag ttcgtacagc ccgtcatgcc gcgcctcgtc gatgcgcggc
     1861 gcctggctgg tcgggccgat gaacaggccg gagaagaggt aggagaggct tggatgccgc
     1921 tgccaatgca ggatcaggct gcgcagcagg tcgggtcggc gcagaaacgg gctgtcgccc
     1981 ggcgtctggc cgccgaccac gacatggttg ccgccgccgg tgccggtgtg acgcccgtcg
     2041 atcatgaatt tatctgcgcc gaggcggcac tgccgggcct cctcgtagac cgtttcggtc
     2101 gtggcgacgc attcgcgcca ggatgaggcc gggtggatgt tgacctcgat cacgccgggg
     2161 tccggggcca cccgaatgac gttgacgcgc ggatcgtgcg gcggcgcgta gccttccagt
     2221 tg
//
LOCUS       DATDEU010000167         5013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1632969, whole genome shotgun sequence.
ACCESSION   DATDEU010000167 DATDEU010000000
VERSION     DATDEU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632969"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1126)
                     /locus_tag="VIV63_04140"
     CDS             complement(119..1126)
                     /locus_tag="VIV63_04140"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6123817.1"
                     /translation="MKGRIRAAALTVCASVLLGACGSGPEGEVKLLNASYDPTRELYE
                     DINVAFAKHWKETTGQEVRISQSHGGSGRQARAVIDGLPADVVTLALAGDIDTIASQG
                     KLLPLNWQQRLPHNSSPYVSTIVFLVRAGNPKNIRDWGDLVRPDVQVITPNPKTSGGA
                     RWNYLAAWAWAEKQPGGNEASAQEFVRKLYKNVPVLDTGARGSLTTFAQRGIGDVAIS
                     WENEAHLAMRERGNSVLQLVVPSISVLAEPSVAVVDKVVLRRGTRAVAQSYLEFLYTP
                     QGQEIAAQNFYRPTDPTVAAKYASTFAQIELVDIQHFGGWKLVQKQHFATGGVFDQIT
                     Q"
     gene            complement(1164..2627)
                     /locus_tag="VIV63_04145"
     CDS             complement(1164..2627)
                     /locus_tag="VIV63_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEY6123818.1"
                     /translation="MQATSHGVVTATLSDPDEWRVAIIGAGFSGTALAIQLLREAAST
                     VSRRSLRIELIDPRPEIGAGVAYATRDYPYPLNVAAGQMSVDGKRPGDFLDYLKSQGI
                     HAAASDYLPRQVYGDYLRARFEEARTAAPSHVEVVHHRASAWQLRRNDGRWVLWLDNG
                     SAVAANDVVLALGNPPPATPPELAHLVYNDRFIRDPWSIGNLANQEIGSVLLVGSGLT
                     MIDAALRLAALRPRVRHIHVLSRHGLMPQSQASDPLPAIKPNVSRALDAARGSTRRLV
                     SAFRALTRSVDDAGGDWREVLALARSQLVSQWQALDHTQRGRFLRHARAAWEVNRHRV
                     PAGPLGAVRNLSRSGVLDVHAGRLEKVNALDDALEVIWRPRGATRARAWLVDRIVNCT
                     GPDCRVDRLTDPLVQSLLSSGVICPDPLSLGILIDARGQPISRDGAPVDRLYYLGPWL
                     RARDWEATAVPELREHAAALARSLAHRNLVESQRRNC"
     gene            2791..3783
                     /locus_tag="VIV63_04150"
     CDS             2791..3783
                     /locus_tag="VIV63_04150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY6123819.1"
                     /translation="MRSTLSLVLALSLSVAAHAAPEKMQAVEVGQGGALSVQTRPVPR
                     PGTGEVLIKVRAAGVNPVDWKSAQRRLGMVPGADVSGTIDMLGEGVTGWKVGDQVLGV
                     ARQSGGYAEYAVIPVTTLARKPKSMTFEQAAGVPIAAETAYRALHETGKIARGQTVLI
                     HGAAGGVGSAAVQIAKAAGARVIGTASPNNHEFLRSIGADQVIDYKTQRFEDVVKNVD
                     LVLNTVDADTNARSVSVVREGGILVSVVGAPNPAACAAAKIRCGRPDRETGAPPADMM
                     AKVVELADAGKFKVHVDGTYTLADAAKAWVKSREGHTRGKLIIQVSEGPTMKHQ"
     gene            3803..>5013
                     /locus_tag="VIV63_04155"
     CDS             3803..>5013
                     /locus_tag="VIV63_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6123820.1"
                     /translation="MEKGVSRVALAAVLGAFCVQVQAAAPALENAARVLGVSQISSLE
                     FEARGRYFQFTQAPAPDLPWPPFDVDGYVATLDFGRATVHAKYHRVQVQEPGRERPHA
                     EATMDQYAAEGMAWNLAPGPVAIPTNLAERNAELWASPQGFIKSALSHYSVVTAQADG
                     SVRVVVTRDDGSFYDGVVSAAGEVLVVRALMDAPVLGDTPMEFRYSEYRDFNGVRFPA
                     RIERRVADLPWYDLAVSEVRINTAKPIAVPAEVTLNPAPSMTRVDVTELAPGVFMFGG
                     GSHNSVVVQQKSGIVVIEAPLNEARSLAVIAKVRAMFPRRKIRRVINTHAHFDHAGGL
                     RTFVAEGATVVTHERNARYYAQAWQQPRKLNPDLLEKRRRQPRFETFSAKHVIDDADR
                     PIEIHVIEGSG"
BASE COUNT          848 a         1586 c         1713 g          866 t
ORIGIN      
        1 aacttcctga acgaagggcc cggcgaaaag cgaggctcat cacccgcagg ggtcgcagtc
       61 gctcgccctt ctccgatcag ccccgtcccc ttttgctagc aaatcaggaa tgtccccatt
      121 attgggtgat ctggtcgaag acgccgccgg tcgcgaagtg ctgcttctgg acgagtttcc
      181 agccgccgaa gtgctggatg tccacgagtt cgatctgcgc gaacgtagat gcatatttcg
      241 cggcgacggt gggatccgtg gggcgataga aattctgcgc ggcgatttcc tggccttgcg
      301 gcgtgtagag aaattcgagg tatgactgcg ccacggcgcg tgtgccgcgg cgcaggacca
      361 ccttgtcgac cacggccacg gatggctcgg ccagcacgct gatggatggc acgactaact
      421 ggagcacgga gttgccgcgc tcgcgcatgg cgaggtgcgc ctcgttttcc caggagatgg
      481 cgacgtcgcc aatgccacgc tgggcgaagg tcgtcagcga gccgcgtgcg ccggtgtcga
      541 gcaccggcac attcttgtac agcttgcgca cgaactcctg cgcgctcgct tcgttgccgc
      601 ccggctgctt ctcggcccac gcccatgcgg cgaggtagtt ccagcgcgca ccgcccgagg
      661 tcttgggatt cggcgtgatc acctgaacgt cagggcgtac gagatcgccc cagtcgcgga
      721 tgttcttcgg attgcccgcg cgaaccagga acacgatggt ggagacgtag ggcgagctgt
      781 tgtgcggcag ccgctgttgc cagttgaggg ggagcagctt gccctgcgag gcgatcgtgt
      841 cgatgtcccc ggcgagcgcc agcgtcacca cgtccgcggg gagtccgtcg atgacggcgc
      901 gggcctggcg tccggaaccg ccgtgggact ggctgatgcg cacttcctgg ccggtggttt
      961 ctttccagtg cttcgcaaag gcgacgttga tgtcttcgta gagctcgcgc gtcggatcgt
     1021 aggacgcgtt gagcagcttc acttcgcctt cggggccgga gccgcaggcg cccagcaaca
     1081 cggaagcgca caccgtcagc gcggcggcgc ggattctgcc tttcaacatg ggtcccggta
     1141 cctcggagga gcggggcggg agactagcag ttccgacgtt gggattcgac gagattgcgg
     1201 tgcgccagcg atcgcgccag cgctgccgcg tgttcgcgca gttcgggcac cgcggtggct
     1261 tcccagtccc gcgcgcgcag ccagggtccc aggtagtaga gcctatctac cggagcgccg
     1321 tcgcgcgata tcggctggcc cctggcgtcg atcaggattc cgagcgacaa cgggtcggga
     1381 cagatgactc cgctcgacaa cagcgactgc accagtggat cggtgagtcg atcgacgcgg
     1441 cagtcgggtc ccgtgcaatt gacgatgcgg tcgaccagcc aggcgcgcgc gcgtgtggcg
     1501 ccgcgcgggc gccagatgac ttcgagagcg tcatcgagcg cattcacttt ctcgagacgg
     1561 ccggcgtgta catcgagtac gccggagcgc gacagattgc gcacggcacc cagcggtccg
     1621 gcgggcacgc ggtgtcgatt cacttcccag gccgcgcgcg cgtgccgcag gaatcgtccg
     1681 cgttgcgtgt gatcgagcgc ctgccactgc gagacgagct ggctgcgcgc cagcgccagc
     1741 acttcgcgcc agtcgccgcc ggcatcgtca acggatcggg tcaatgcacg aaacgcactg
     1801 accagccggc gcgtcgagcc tcgcgcggca tcgagagcgc gggacacatt gggcttgatc
     1861 gcggggagtg ggtcggacgc ctgtgactgc ggcatcaggc cgtgccgcga caacacatga
     1921 atgtgccgca cgcggggtcg gagcgccgcg agcctgagcg cggcgtcgat catggtgagc
     1981 ccgctgccta cgagcaatac gctgccgatc tcctggttcg cgagattgcc gatactccag
     2041 ggatcgcgga tgaatcggtc gttgtaaacg agatgcgcga gttcgggcgg cgtagccggc
     2101 ggtggattac ccagcgccag cacgacgtcg ttcgccgcca cggcgctgcc attgtcgagc
     2161 cataacaccc atcgtccgtc attgcgccgc agctgccagg cgctggcgcg gtggtgcacc
     2221 acttcgacgt gagacggcgc ggccgttcgc gcttcctcga agcgcgcacg tagatagtcg
     2281 ccatagacct gtcgcggcag gtaatcgctg gcggcggcgt gaatgccctg gctcttgaga
     2341 tagtcgagga agtcgccagg acgtttgcca tcgaccgaca tctggccggc ggcgacgttc
     2401 agcggataag gatagtcgcg ggtcgcgtag gccactcccg cgccgatctc ggggcgcgga
     2461 tcgatgagct cgatgcgcag gctgcgccgc gatacggtgg aagccgcctc ccgcaagagt
     2521 tgaatggcca gcgcggtccc ggaaaatccc gcgccaatga tggcaacgcg ccattcatcg
     2581 ggatcggata gggtggcggt gaccactcca tggcttgttg cctgcatgag ttgaagtatc
     2641 ggaaccacat ggtcacagcg tcgattccgg gcgggtatga atccggttac gtcatttggg
     2701 cgggaccgtg cacttttcgc cgattggccg gtctcatgtc cgctaccggt tcaccgacgc
     2761 cggtacacat taataatgag gagcttcttg atgcgttcca ctttgagtct cgttctcgcg
     2821 ctgtcgctca gcgtggcggc ccacgcggca cccgaaaaaa tgcaggccgt ggaagtgggc
     2881 cagggtggag cattgtcggt gcagacgcgg cccgtgccgc gcccgggcac aggcgaagtg
     2941 ctcatcaagg tgcgtgccgc cggtgtcaat cccgtggact ggaagtcggc acagcgccgc
     3001 ctcggcatgg tgccgggcgc ggatgtatcg ggaaccatcg acatgctggg cgaaggcgtc
     3061 accggctgga aggtgggtga tcaggtgctc ggggttgctc gccagagtgg cggctacgcg
     3121 gaatacgccg tcatcccggt gacgaccctc gcgcgcaagc cgaaatccat gaccttcgaa
     3181 caggccgccg gcgtgcccat cgccgccgag acggcgtatc gcgcgttgca cgagaccggc
     3241 aagatcgcac gcggccagac cgtgctcatt cacggcgccg cgggaggcgt gggttcggcc
     3301 gccgtgcaga tcgccaaggc cgccggcgcg cgcgtcatcg gcaccgcctc gcctaacaac
     3361 cacgagttcc ttcgctcgat cggcgccgac caggtcatcg actacaagac gcagcggttc
     3421 gaggacgtgg tgaagaatgt cgacctcgtt ctcaacaccg tcgatgccga caccaacgcg
     3481 cgttccgttt ccgtggtgcg cgagggcggg atcctggtct cggtcgtcgg ggcgccgaat
     3541 cccgccgcgt gcgccgccgc gaaaatccgt tgcggccggc cggatcgtga gacgggcgcg
     3601 ccccccgccg acatgatggc taaagtcgtg gagctcgcgg atgccggcaa gttcaaggtg
     3661 cacgtcgatg ggacctatac gctggccgac gccgccaaag cctgggtcaa gagccgcgaa
     3721 gggcacactc gtggcaagct catcatccag gtgtcggaag gcccgacgat gaagcatcag
     3781 tagaacttcg aagggggagc cggtggagaa gggggtatca cgcgtcgcgc tggcggccgt
     3841 gctcggagcg ttttgcgtcc aggttcaggc agccgcgccg gccctcgaga acgccgcgcg
     3901 ggtgctcggc gtctcgcaga tcagcagtct cgagttcgag gctcgcggca gatacttcca
     3961 gttcacgcaa gctcccgcac ccgaccttcc ctggccgcca ttcgatgtcg atggttacgt
     4021 cgccacgctc gatttcgggc gcgccaccgt gcatgcgaag taccaccgcg tgcaggttca
     4081 ggaacccggg cgtgagcggc cgcacgccga ggcgacgatg gatcagtacg ccgccgaagg
     4141 catggcgtgg aacctggcgc ccgggcccgt cgccattccc accaatctcg cggagcgcaa
     4201 cgccgagctc tgggcatcgc cccagggttt catcaagtcg gcgctgtctc actactcggt
     4261 cgtgacggcg caggccgacg ggagtgttcg cgtcgtggtc actcgcgacg acggctcgtt
     4321 ctacgacggt gtggtcagcg cggcgggtga agtgctcgtg gtgcgcgcac tcatggatgc
     4381 gcccgtgctc ggcgacacac ccatggagtt tcgctactcg gagtatcgcg atttcaacgg
     4441 cgtgcgcttc ccggcgcgta tcgaacgccg ggtggcggat ctgccgtggt acgacctcgc
     4501 cgtgagtgag gtgcgtatca acaccgcgaa gccgattgcg gttcccgctg aagtcacgct
     4561 gaacccggcg ccgtccatga cgcgcgtcga tgtcaccgag cttgcgccgg gtgtattcat
     4621 gttcggcggc ggttcgcaca acagcgtggt ggtgcagcag aagagcggca tcgtcgtcat
     4681 cgaagcgcca ctcaacgaag cgcgctccct cgccgtgatt gccaaggtgc gcgcgatgtt
     4741 tccgcgccgg aagatccgcc gcgtgatcaa cacgcatgcg catttcgatc acgcgggagg
     4801 cctgcggact ttcgtcgcgg agggcgcgac ggtcgtcacg cacgagagaa acgctcgtta
     4861 ctacgcgcaa gcgtggcagc agccgcgcaa gctcaacccg gatctgctgg aaaaacgccg
     4921 gcgtcagccg cgcttcgaaa cgttttccgc gaagcatgtg atcgacgatg ccgatcggcc
     4981 catcgagatc cacgtcatcg agggcagtgg cca
//
LOCUS       DATDEU010000168         7007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2071104, whole genome shotgun sequence.
ACCESSION   DATDEU010000168 DATDEU010000000
VERSION     DATDEU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071104"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VIV63_04160"
     CDS             <1..1058
                     /locus_tag="VIV63_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123821.1"
                     /translation="LIYRLAVSAAMGSTFSTSVVAWQALAMVGSVAAGFLLAHLYIRV
                     VERVTDVPSAIVMQFVGAFGIWILADEIGLSAIVTVVTFAITAARITPERTPASLRLP
                     SYAVWETVVFVLNVLAFVAIGLQVRPILDALEGDEQTHYLQIAGLVLATVVCVRIAWV
                     LIYNRAAWLKNRFFGAGSWPGEYIPTLAGSTIVGWCGMRGIVTLATAFALPPAFPYRE
                     LILLCAFTVVAGTLVAQGLTLRPLILLLGLKDDGTVEREIHIAQAQLARVAAEIIDKD
                     DSDAAQMLRAEFAMPTDGEVAEGTETEHSVRNRLRARIIAAQRRDLVRMRRAAEIGDD
                     AFHRIEERLDRTEVNTR"
     gene            1164..1544
                     /locus_tag="VIV63_04165"
     CDS             1164..1544
                     /locus_tag="VIV63_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123822.1"
                     /translation="MTEPLRRMLALDGITCLLMGLVLVLAADELSKLFALPVNLLVYA
                     GILLFPCAALMLAIAGRPWPPSTLVWMVIAGNALWVLASLVVALVLFEPSALGLSFLL
                     LQAAVVAVLLVYELRGLKARVSVR"
     gene            complement(1531..1983)
                     /locus_tag="VIV63_04170"
     CDS             complement(1531..1983)
                     /locus_tag="VIV63_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123823.1"
                     /translation="MRRRLVGGEILEVPVGDRFGYVQFLGVHPDYGDAVLVNPALHDR
                     QGRFASDFFSTGYVTFYPAAEAVASKKVEVVAQSSPPKLPKRFRRAKERDGAVESWVI
                     EGGWRKVVKETLSDEERRLPIASVWKHEHLRAQIASGWTPETDVASGR"
     gene            2101..4428
                     /locus_tag="VIV63_04175"
     CDS             2101..4428
                     /locus_tag="VIV63_04175"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY6123824.1"
                     /translation="MKSRKSQSNKTPPSTVSGPSRKPRSPANDPQGRKADATNALAKS
                     VPANPTKASEYGDAARKPTAGAHRDAPPEVTGSTLSENNSSAERLDRVRADASGQVLT
                     TNQGVPIADNQNSLKAGLRGPALLEDFILREKITHFDHERIPERIVHARGSAAHGYFE
                     CYAPQTKITRASVFAEAGKRTPVFVRFSTVAGERGSADTVRDVRGFAVKFYTDAGNWD
                     LVGNNMPVFFIQDAMKFPDLIHSVKPEPHHGMPQAASAHDTFWDFVSLMPESVHMQMW
                     AMSDRAIPRSYRMMQGFGVHTFRMVNARGDSVFVKFHWNPVAGTHSLDWDEAVKLCGA
                     DADFHRRDLWEAIEAGAYPEWELGLQVFTEEQAEKFSFDVLDATKIVPEELVPVKPIG
                     KMVLNRNPDNFFAETEQVAFCTAHIVPGLDFSNDPLLAGRIHSYVDTQISRLGGPNFH
                     EIPINSPVAPVANNQRDGMHRQAINRGRVAYEPNSLGGGCPFQAGAQGFTSFPQPINE
                     DKVRGKPEKFADHYTQAALFFDSQTPIEQAHIVRAFRFELTKVQVPAIRERVVSQLVN
                     VNADLARKVAEGLGIDVPKAQPPVRPRNLKPEVKTSPALSLFARPGDGSIRTRCIALL
                     VADGVQGDSLKKLHDKLSDEGAVPRYVGIRLGKVKTADGQPIEAEVTLETAPSVVFDA
                     MVVPDGEGGVTALTKVGQALEFVKDQFRHCKTILALGAGSQLLDAAGIPPAKKDKAPD
                     GGLLRLAAGDAAAKAFIAAVAKHRHFERAQDPPPV"
     gene            4425..4814
                     /locus_tag="VIV63_04180"
     CDS             4425..4814
                     /locus_tag="VIV63_04180"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEY6123825.1"
                     /translation="MTSSAIAADAVMAFHFAFIVFALCGSFLILRWPKVIWLHLPALA
                     WGAYIELSGEICPLTPLENHFRELAGEGSYYGGFITHYLGPIIYPANLTRGGQYLAFA
                     ILVAFNVTGYALVWRQRRRLRRSSVAL"
     gene            complement(4744..6678)
                     /locus_tag="VIV63_04185"
     CDS             complement(4744..6678)
                     /locus_tag="VIV63_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HEY6123826.1"
                     /translation="MARDAGKLSRRTITQDGQMRSAERRCDDYRNAFQLLLDEPFDDL
                     DKTIERMLKVLATTLNVGRVGFWTVDTNYTTIRCEHVFSVDSRRGLGLTMLKRSDCPA
                     YFDSLCQELVVAVEDARKDPRTRELSASYLEPLGVQSLLDVPVRAFGRYVGVLCHEQF
                     DSKRSWTREDEHFASGVATQIALAYERDRAKRAQEKLLERSLRDEDSQLANRLHLEQA
                     LTAYLQNPARAGAVLIMSGDQHSFVASCLGSRRMQVLLRQFAGRLAIAGPEGTLVARV
                     GTNEFAMLLRGVEEWEIPRILTQVNACLTTPLVSEGERLFFTLSGGYSIIDSETEQDA
                     ETLLTEAQMACHEARAAGGNRASPFTAEMRQSMRGRISLEQDLRRALDAAEFDLHFQP
                     IVPLKRGGEMSLEALLRWRHPRLGVLAPAAFIQVAIDSGVMLELGRRVLGAACSSLAK
                     LRSQKGLEQICMTVNMSAPEVLLPGTADAIHMELLRSGLPPQALTIEITETALMVDLD
                     RAAAAITEIREIGVSISLDDFGTAYSSLCWLRKLTIDKIKIDRSFVAGIEHEPKDLAI
                     VRSIIELSRVFGRNLVAEGVENLEQLRILRELGVDHAQGFLFARPEPLESFDAARLKA
                     LLTIDEAAGADATPARIQSR"
     gene            complement(6751..>7007)
                     /locus_tag="VIV63_04190"
     CDS             complement(6751..>7007)
                     /locus_tag="VIV63_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123827.1"
                     /translation="TVLVSLVSAFPQLVWLSEHKAGVFGIEGILLAASGVVIWRARRL
                     PCPVDPAAARTCMRLRRTSLALFFVSLAMFLGGATFAFVL"
BASE COUNT         1291 a         2303 c         2134 g         1279 t
ORIGIN      
        1 tgctgatcta ccggctcgcc gtcagtgcgg cgatgggcag tacgttcagc accagcgtcg
       61 tcgcctggca ggcgctggcg atggtcggca gcgtagccgc cggtttcctg ctcgcgcatc
      121 tgtacatccg cgtcgtcgaa cgcgtgaccg acgtgccaag cgccatcgtc atgcagttcg
      181 tcggcgcgtt cggcatctgg attctcgccg acgagatcgg tctttcggcc atcgtcaccg
      241 tggtcacgtt cgcgatcacc gccgcgcgca tcacgcccga gcgcaccccc gcatcgctgc
      301 ggctgccgtc atacgcggtg tgggaaaccg tggtcttcgt actcaacgtg ctggcgttcg
      361 tggcgattgg cctgcaagtt cggcccatcc tggatgcgct cgaaggcgac gaacagacgc
      421 actatctgca gatcgccggt ctcgtactgg ccaccgtagt ctgcgtgcgc atcgcctggg
      481 tgctcatcta caaccgcgcg gcctggctca agaaccggtt cttcggcgcg ggtagttggc
      541 ccggcgagta cattccgacg ctggccggca gcaccatcgt cggctggtgc ggcatgcgcg
      601 gcatcgtcac gctggccacc gcgttcgcgc tgccgcccgc attcccctat cgcgaactca
      661 tcctgctctg cgcgttcacc gtagttgcgg ggacactcgt cgcccaggga ctcacgcttc
      721 gaccgctgat cctgttgtta ggcctcaagg acgacggcac ggtggagcgc gaaatccaca
      781 tcgcgcaggc gcagttggcg cgcgtcgcag ccgagatcat cgataaagac gattccgacg
      841 ccgcgcagat gttgcgcgcg gaattcgcga tgccgaccga tggagaagtc gctgaaggta
      901 cggagaccga acactccgtg cgcaaccggc tgcgcgctcg catcatcgcc gcgcagcggc
      961 gagatctcgt gcgcatgcgc cgcgccgccg agatcggcga cgacgcgttt catcgcatag
     1021 aggaacggct cgatcgcacg gaagtgaata ctcgctagcg aagaaccccg gtgatgcgca
     1081 cgcgcaatta cccgcgaggt aattgaggcg gctacgcagc ttcgtcacag tgcgcccatc
     1141 gcaagaatgt gaaggagctg gaaatgactg agcccttgcg ccgaatgctc gcgctggacg
     1201 gcatcacttg tctgctcatg ggactcgtgc tggtcttggc ggcggatgaa ctgtcgaaac
     1261 tcttcgcgct gcccgtcaat cttcttgtct acgcgggaat tctgctgttc ccctgcgcag
     1321 cgttgatgct cgcgattgct ggcagaccct ggccgccgtc cacgctggtg tggatggtga
     1381 tcgccggcaa tgcgctctgg gtgctggcca gcctcgtcgt agcgctggtt ctgtttgagc
     1441 ccagcgcgct gggcctgtcg ttcttgctgc tgcaagcggc cgtggtcgcc gtcctgctcg
     1501 tctacgaact tcggggattg aaagcccggg tcagcgtccg ctagcgacgt cggtttccgg
     1561 cgtccagccg ctcgcgatct gcgcgcgcag gtgctcgtgt ttccacacgc tcgcgatcgg
     1621 caggcgtcgt tcttcgtcgg acagagtttc cttcacgacc ttgcgccagc cgccttcgat
     1681 gacccacgac tcaaccgcac catcgcgctc tttcgcacgg cgaaagcgct tgggaagttt
     1741 cggcggcgat gactgggcga cgacttcgac cttcttgctg gccacggctt cggcggccgg
     1801 atagaacgtc acgtagcccg ttgaaaaaaa gtccgaagcg aaccggccct ggcgatcgtg
     1861 cagcgcggga ttcacgagca ctgcatcgcc atagtcgggg tggaccccga gaaactgtac
     1921 atagccgaag cgatctccca cgggtacttc gaggatttcg ccgcccacca atcgtcttcg
     1981 catctgtgcc atgggctcat cttgtcacgt aatgaggtcg gacattgacg gcgattgact
     2041 cccgtttcta agcgtctgca cctacagcgt cggcttggtc aaaagagcgc taatggccgc
     2101 atgaaatcac gcaagtcgca gagcaataaa accccgccca gcaccgtcag cggtccgtca
     2161 cgcaagccac gttcgcccgc gaatgacccg cagggcagga aggcggacgc taccaatgca
     2221 ctggcgaagt ccgtacccgc caatccgacc aaggcgtcgg agtacggtga tgcggcgcgc
     2281 aagcccaccg caggtgcaca tcgcgacgcg ccgcccgaag tcacaggcag cacgctcagc
     2341 gagaacaact cttcagccga gcggctcgat cgggtgcgcg ccgatgcgtc cggacaggtg
     2401 ctcaccacca accagggtgt gccgatcgcc gacaaccaga actcgctcaa ggcgggcctt
     2461 cggggccccg cgcttctcga agacttcatc ctgcgagaga agatcacgca cttcgatcac
     2521 gagcgcattc ccgaacgtat cgtccacgct cgcggctccg ccgctcatgg ctacttcgag
     2581 tgttatgcgc ctcagacgaa gatcacacgc gcctcggtgt ttgccgaagc cggcaagcgc
     2641 acccccgtgt tcgtccgctt ctccaccgtc gcgggcgaac gcggctcggc cgataccgtg
     2701 cgcgacgtgc gcggcttcgc cgtgaagttc tataccgacg caggcaactg ggatctcgtc
     2761 ggcaacaaca tgcccgtgtt tttcatccag gatgcgatga agtttccgga cctcatccac
     2821 tccgtcaaac ccgagccgca tcacggcatg ccgcaggctg ccagcgcgca cgatacgttc
     2881 tgggacttcg tgtccctgat gcctgaatcc gtgcatatgc agatgtgggc gatgtcggat
     2941 cgcgccattc cacgcagcta tcgcatgatg cagggcttcg gcgtgcacac cttccgcatg
     3001 gtcaacgcac gcggcgacag cgtgttcgtg aagttccact ggaatccggt cgccggcacg
     3061 cattcgctcg actgggacga agcggtcaag ctctgcggcg ccgatgcgga tttccaccgc
     3121 cgcgatctct gggaggccat cgaagccggc gcctatcccg agtgggaact cggcctgcag
     3181 gtattcacgg aagaacaagc cgagaagttc agtttcgacg tgctcgacgc cacgaaaatc
     3241 gtgcccgaag aactggtgcc ggtgaagccg atcggcaaga tggttctcaa ccgcaatccc
     3301 gacaacttct tcgcggaaac cgagcaggtc gcattctgta cggcgcacat cgtgcccggc
     3361 ctcgacttca gcaacgatcc gctgctcgcc ggacgcatcc actcctatgt ggacacgcag
     3421 atctcccggc tgggcggacc taacttccac gagattccca tcaacagtcc ggtggcgccc
     3481 gttgccaaca accagcgcga cggcatgcat cgccaggcca tcaaccgcgg ccgcgtcgcg
     3541 tacgaaccga attcactcgg cggcggctgc ccgttccagg cgggcgcgca gggtttcact
     3601 tcgtttcctc agcccatcaa tgaagacaag gtgcgaggca aacccgaaaa gttcgccgat
     3661 cactacacgc aggctgcgtt gttcttcgac agccagacgc ccatcgaaca ggcacacatc
     3721 gtgcgcgcct tccgtttcga gctcacgaaa gtgcaggtgc cggcgattcg cgagcgagtc
     3781 gtgtcacaac tcgtgaacgt gaacgccgac ctcgcgcgca aagtcgcaga aggcctgggt
     3841 atcgatgtgc cgaaggccca gcctccggtt cgtccccgca acctgaaacc cgaagtgaag
     3901 acttcgcctg ccctgtcgct gttcgcgcgg ccgggtgacg gcagtatccg cacgcgttgc
     3961 attgcgttgt tggtcgccga tggcgtccag ggcgactcgc tgaaaaagct gcatgacaaa
     4021 ctgtccgacg aaggcgccgt gccgcgctac gtgggtatac gcctcggcaa ggtcaagacc
     4081 gctgacggtc agccgatcga agcagaagtc acgctcgaaa ccgctccgtc cgtggtattc
     4141 gatgccatgg tcgttcccga tggcgagggc ggagtcacgg cactcaccaa ggtgggacag
     4201 gcgctggagt tcgtgaagga ccagttccgt cactgcaaga ccattctcgc gctcggcgcc
     4261 ggcagccaac tactcgatgc tgctggcata ccaccggcga agaaagacaa ggcgcccgat
     4321 ggtggcctgt tgcggcttgc ggccggcgac gccgccgcga aggcgttcat tgcggcagtg
     4381 gccaaacatc gtcacttcga gcgcgcgcag gacccgccgc ccgtttgact tcgagcgcca
     4441 ttgccgccga cgccgtcatg gcgtttcact tcgcgttcat tgtttttgcg ctgtgcggat
     4501 cattcctgat cctgcgctgg ccaaaggtga tttggctgca tctgcctgcg ctcgcgtggg
     4561 gcgcgtacat cgaactcagc ggtgaaatct gcccgctgac gccgctcgag aatcactttc
     4621 gggaactggc gggagaaggt agttactacg gcgggttcat cacccactac ctcgggccca
     4681 tcatctatcc cgcgaatctc acgcgcggcg ggcaatacct ggcgttcgcg atcctggtcg
     4741 cgttcaacgt gactggatac gcgctggtgt ggcgtcagcg ccggcggctt cgtcgatcgt
     4801 cagtagcgct ttgagccggg cggcatcgaa gctttcgagc ggctcgggcc gcgcgaacag
     4861 aaagccctgc gcgtgatcca cgcccaactc gcgcaggatg cgcagctgct ccaggttctc
     4921 cacgccttcg gcgaccagat tgcgtccgaa tacacgcgag agttcgataa tggaccgcac
     4981 gatggccagg tctttgggct cgtgctcaat gccggcgacg aatgaccgat cgatcttgat
     5041 cttgtcgatg gtcagcttgc gcagccagca cagcgatgaa tacgccgtgc cgaaatcgtc
     5101 caggctgatg ctcacgccga tctcacggat ttcggttatg gccgccgcgg cgcgatcgag
     5161 gtcgaccatg agcgcggtct cggtgatctc gatggtcagc gcttgcggcg gcagaccact
     5221 gcgcagcagc tccatgtgga tggcgtcggc cgtacccggc aacaacactt ccggcgccga
     5281 catgttgacg gtcatgcaga tctgctcgag tcccttctgg cttcgcagtt tcgccaatga
     5341 actgcaggcc gcgcccaaca cgcgccgccc gagttcgagc atgactccgg aatctattgc
     5401 cacctggatg aaagcggcag gcgcgagaac acccagccgc gggtgccgcc agcgaagcag
     5461 cgcttccagc gacatctcgc cgccgcgctt caacggaacg atgggctgga aatgcagatc
     5521 gaattccgcg gcatcgagtg cgcgccgcag gtcttgctcc agtgagatgc ggccgcgcat
     5581 ggactggcgc atctcggccg tgaacggcga cgcgcgattg ccgccggccg cccgcgcttc
     5641 gtgacaggcc atctgcgctt cggtcaacag ggtttcggca tcctgctccg tttccgagtc
     5701 gatgatcgag taaccgccac tcagcgtgaa gaacaaccgt tccccttcgc tcacgagcgg
     5761 cgtcgtcagg catgcattca cctgcgtgag gattcgcgga atttcccatt cttcgacgcc
     5821 gcgcagcagc atcgcgaact cgttggtgcc gacgcgcgcg accagcgtgc cctcgggtcc
     5881 cgcaatcgcg aggcggccgg cgaactgacg cagcaatacc tgcatgcgcc gcgaccccag
     5941 gcaactggcc acgaaagaat gctgatcgcc ggacatgatg agcacggcgc cggcgcgtgc
     6001 ggggttctgc agataggcgg tgagcgcctg ttcgagatgc aggcggttcg ccaactgcga
     6061 atcttcatcg cgcagcgaac gctcgagcag tttttcctgc gcccgcttgg cgcgatcgcg
     6121 ctcgtaggcc agtgcgatct gcgtcgcgac gccggaggcg aaatgttcgt cttcgcgcgt
     6181 ccacgaacgt ttgctgtcga actgctcgtg gcacaacacg ccgacgtacc gcccgaacgc
     6241 gcgcaccggc acatcgagca gtgactggac gccgagcggc tccaggtacg acgccgacaa
     6301 ttcgcgcgtg cgtggatcct tgcgcgcgtc ttcgacggcc accaccagtt cctggcacaa
     6361 cgagtcgaag taggcaggac aatccgaacg cttcagcatc gtcagaccca ggccacgcct
     6421 ggagtcgacg gagaacacgt gttcgcaacg gatcgtggtg tagttggtgt cgacggtcca
     6481 gaaaccgacg cggccgacat tgagcgtggt ggcgagcacc ttgagcatgc gctcgatcgt
     6541 cttgtcgaga tcgtcgaagg gttcgtcgag caggagctgg aaggcattgc gatagtcgtc
     6601 gcaacggcgt tccgccgagc gcatctgccc gtcttgcgtg atcgttcgcc gactcaactt
     6661 tccagcgtct cgagccatgc gcctctccct tgcgcggcca agctcaaaac cactgtacgc
     6721 ctgccgcgtc cgcctcgcta ctgaccggca tcacagaacg aaagcgaagg tcgcaccccc
     6781 cagaaacatt gcaagcgaaa caaagaacaa ggcgaggctg gtccgccgca gccgcatgca
     6841 agtgcgcgcc gccgcagggt ccaccgggca tggaagacgc cgtgcccgcc agatcacgac
     6901 cccactcgcc gccagcaata tgccctcgat gccgaatacc ccggccttat gctcggacag
     6961 ccagacgagc tgcggaaacg ccgacaccag gctcacgagc accgtgc
//
LOCUS       DATDEU010000169         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2071239, whole genome shotgun sequence.
ACCESSION   DATDEU010000169 DATDEU010000000
VERSION     DATDEU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071239"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2707
                     /locus_tag="VIV63_04195"
     CDS             <1..2707
                     /locus_tag="VIV63_04195"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY6123828.1"
                     /translation="SHTGRALREYEDAIGKKPVPMPESQVAESPERYAARNSIVIHNA
                     REHNLRNIDVTIPREAVTVITGVSGSGKSTLAFDIIFNEGQRRYLESLNAYARQFVQP
                     AARPDVDAIYGIPPTVAIEQRTSRGGRKSTVATLTEIYHFLRLLYVKLGTQFCPKCDV
                     AIEPQTASTIAERLLRQHQDSRILLLAPLVVARKGYYTELAKWASARGHKQLRVDGEM
                     MSTSQFPRLNRFKEHTIELPMNQINVQKDSADLLERNVIRALDMGKGLLHVLDLDSTA
                     KKPKVQVYSVKRSCPSCGTSFAELDPRLFSFNSKHGWCEGCFGTGLELEGFDEEQSGE
                     ERAWSDSADGEVHTCETCDGQRLNQIALHVRFRDLNIADLTHLAIDETVSFVSKLRLA
                     KREAEIARDLMAEIKSRMAFLQRVGLGYLPLDRSAPTLSGGEAQRIRLAAQLGSNLQG
                     VCYVLDEPTIGLHPRDNKVLLDALAELAKNRNTLVVVEHDEDTIRRADHVIDLGPGAG
                     VRGGEIVAEGTIEQLKKNPASITGRFLKNPLKHSAEPRRATGPRAPRLILDDVDLHNV
                     RKEDIAVPVGRLVVVTGVSGSGKSTVARDVLYANLKRLTAKLPRGAKKPEITGCATVR
                     GAEHIDRVLEVDQTPIGKTPRSCPATYIGFWDDIRRIFANTTEAKIAGYSTSRFSFNT
                     GEGRCPACEGQGMQTIEMNFLPDVKVLCDSCRGRRFNSETMSILWRGKSVGDLLAMNI
                     DDAVEFFAAHASIHHALRLLQDVGLGYLTLGQPSPTLSGGEAQRIKLVTELAKVRGEE
                     RRAPRQKQQHTLYVLDEPTVGLHMADVEKLIHVLHRLVDAGNSVVVVEHNLDVMAEAD
                     WIIDMGPEAGAEGGKVVVAGPPSVVMKASASHTGTVLKEFLRDRR"
BASE COUNT          547 a          956 c          895 g          456 t
ORIGIN      
        1 ctcgcacacg ggtcgagcgc tgcgcgaata cgaagacgcc atcggcaaga agccggtgcc
       61 catgcccgaa agccaggtcg cggagagccc ggagcggtac gccgcgcgca actccatcgt
      121 catccacaac gcgcgcgaac ataacctgcg caacatcgac gtgacgatcc cgcgcgaagc
      181 cgtcaccgtc atcaccggtg tgtcgggctc gggcaaatcc acgctggcgt tcgacatcat
      241 cttcaacgaa ggccagcgcc gctatctcga atcgctgaac gcctatgcgc gccagttcgt
      301 gcagcccgcg gcgcggccgg acgtggatgc gatctacggc atcccgccca cggtcgccat
      361 cgagcagcgc accagccgcg gtggacgcaa gagcacggtg gccacgctca ccgagatcta
      421 ccacttcctg cggctgctct acgtgaagct cggcacgcag ttctgcccca agtgcgatgt
      481 cgccatcgaa ccgcagacgg cgtccaccat cgccgagcgg ctgctccggc agcaccagga
      541 ttcgcgcatc ctgctgctgg cgccgctggt cgtcgcccgc aagggttatt acaccgagct
      601 cgcaaaatgg gcgagcgcgc gcggccacaa acaactgcgc gtcgacggcg agatgatgtc
      661 gacgagccag ttcccgcggc tcaaccgctt caaggaacac accatcgaac tgccgatgaa
      721 ccagatcaac gtgcagaagg acagcgccga cctgctcgag cgcaatgtca tccgcgcgct
      781 ggacatgggc aaaggcctgc tgcacgtact cgatctcgat tccaccgcga agaagcccaa
      841 ggtgcaggtg tacagcgtca aacgctcctg cccttcctgc ggtacgagtt tcgccgagct
      901 cgatccgcgg ctgttctcgt tcaattccaa gcatggctgg tgcgaagggt gcttcggcac
      961 cggtctcgag ctcgaaggct tcgacgagga acagagcggc gaagagcgcg cgtggagcga
     1021 ctccgccgac ggcgaagtgc acacctgtga aacctgcgat gggcagcggt tgaaccagat
     1081 agcgctgcac gtgcgtttcc gcgacctcaa catcgccgat ctcacgcacc tcgccatcga
     1141 cgaaaccgtg agcttcgtga gcaagcttcg cctggcgaag cgggaggcgg agatcgcgcg
     1201 cgatctcatg gcggagatca agtcgcgcat ggcgttcctg caacgcgtgg gtcttggcta
     1261 tctaccgctg gatcgttcgg cgcccacctt gtcgggcggc gaagcgcagc gcatccgcct
     1321 cgcggcgcag ctcggctcga acctgcaggg cgtgtgttac gtgctcgacg agcccaccat
     1381 cgggctgcac ccgcgtgaca acaaggtgtt gctggacgcg ctggccgaac tcgcgaagaa
     1441 ccgcaacacg ctggtcgtcg tcgaacacga cgaggacacg atccgccgcg ccgaccacgt
     1501 catcgatctc gggcccggcg cgggcgttcg cggtggcgaa atcgtggccg aaggcaccat
     1561 cgagcagctc aagaagaacc cggcatcgat cacgggccgg ttcctcaaga atccgctgaa
     1621 gcattcggcc gaaccgcgcc gcgccaccgg ccctcgcgcc ccgcggctca ttctcgacga
     1681 cgtggatctg cacaacgtcc gcaaggaaga catcgccgtg cccgtcggca ggctggtggt
     1741 ggtcacgggg gtgtcgggtt ccggcaaatc cacggtggcg cgcgacgtcc tttacgcgaa
     1801 tctcaagcgg ctcacggcca aactgccgcg cggcgccaag aagcccgaga tcacgggatg
     1861 tgcgactgtg cgcggcgccg aacacatcga ccgcgtgctc gaggtggacc agactcccat
     1921 cggcaagacg ccgcgctcct gcccggctac ctacatcggc ttctgggatg acatccgccg
     1981 catcttcgcc aacaccaccg aggcgaagat cgccggctat tcgaccagcc gtttttcgtt
     2041 caacacgggt gaaggacgct gcccggcctg tgaaggccag ggcatgcaga ccatcgagat
     2101 gaacttcctg ccggacgtga aggtgctctg cgattcgtgc cgcggccggc gcttcaattc
     2161 cgagaccatg tcgatcctgt ggcgcggcaa gtcggtcggc gacttgttag ccatgaacat
     2221 cgacgatgcc gtggagttct tcgccgcgca cgcctccatc caccatgcgt tgcgactgct
     2281 gcaggacgtc gggctggggt atctgacgct cggccagccg agccccacgc tgtcgggtgg
     2341 cgaagcccag cgcatcaagc tcgtgaccga actcgccaag gtgcgcggcg aggagcggcg
     2401 tgcgccgcgg cagaagcagc agcacacgct gtacgtgctc gacgaaccga ccgtgggcct
     2461 gcacatggcc gacgtcgaaa agctcatcca cgtgctgcat cggctcgtgg atgccggcaa
     2521 ctccgtggtg gtggtcgagc acaacctcga cgtcatggcc gaggccgact ggatcatcga
     2581 catgggtccc gaggccggtg ccgaaggcgg caaggtcgtg gtcgcgggac cgccctccgt
     2641 cgtgatgaaa gccagcgctt cgcacaccgg cacggtgctc aaggagtttc tgcgcgaccg
     2701 tcgttagggg acactcccca ttttctagca aaaggggccg cacctcgcga gtacatcgtc
     2761 acttcaatcc ttcctgagag gtgcggcccc ttttgcgaga aattggggag tgtcccctaa
     2821 cgacggtcgc gcagaaactc cttgagcacc gtgc
//
LOCUS       DATDEU010000170         3914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_612381, whole genome shotgun sequence.
ACCESSION   DATDEU010000170 DATDEU010000000
VERSION     DATDEU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612381"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1065
                     /locus_tag="VIV63_04200"
     CDS             181..1065
                     /locus_tag="VIV63_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEY6123829.1"
                     /translation="MPELRIILLVVGVLFVAGIAGFEWWRSRGSRPLATGVPRDDGAA
                     DAPPRPAPAPLPDINVVRDSRVAMSDSLPVIELASTSESGTRRALGISISDDVAVDVA
                     HDSGSREPAKPRPEPTRNEPYIGQHDEISEVRIVDEQPVRGPQLVLSWPNETERRIVT
                     LRVIPRVDPRFQGRAVRQAFSAAGFWHGPLDIYHLPDEGGRVVLSAAALAQPGTFDPS
                     IMDSQRFSGLNLFAVLPGPRPERETFDELVHAARQLAERLDGTLADQHGEELTPQRIA
                     RLRQALEPPATSRASGEA"
     gene            1072..3123
                     /gene="ligA"
                     /locus_tag="VIV63_04205"
     CDS             1072..3123
                     /gene="ligA"
                     /locus_tag="VIV63_04205"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY6123830.1"
                     /translation="MAAPKTADSQAAQRAAALRDEINGHDYRYYVLSEPSVPDAEYDR
                     LMKELREIETAHPSLITPDSPTQRVSGVAAAEFGEVTHTIPMLSLENGFSDEDLSDFD
                     RKVRERLGVPGPIEYAAEPKLDGLAISVMYRNGEFLRAATRGDGVTGEDVSANVRTIR
                     GLPARLRGKPPALLEVRGEVFLPFAGFERMNRDAAARGDKLYVNPRNAASGSLRQLDP
                     RITASRPLDLFFYSLGVVEGGGIPDRHSELAAQLNAWGLRTCPEAKKVTGIEGCLDYY
                     RDIGARRARLPYQIDGVVYKVDSRADQEKLGFVSRAPRWALAHKFPADEELTILEDVI
                     FNVGRTGALTPAAKLKPVFVGGVTVSNATLHNMDEVARKGFMIGDTVVVRRAGDVIPE
                     VARYLPEKRPENARPIVMPTICPVCQSPVVRLEDQAVYKCTGGVLKCRAQRAEWIMHF
                     AARRAMDIEGLGEKLIEQLVNDGAVSSPADLYDLEAKSLAERERMGEKSAQNVVDAIT
                     KSKKTTLPRLLFALGIPQVGESTALALAQQFGTVEKLEGATAAEIEETPDVGPIVSRS
                     VFEFFQSELAKSVVERLKRSGVTYTPIAVEARASLPLAGLTIVITGTLAGMQRDAAED
                     ALRELGAKVSGSVSKKTSFLVVGADAGSKLAKAEALGVRILDEAALEQILKTKQPPAP
                     V"
     gene            complement(3162..>3914)
                     /locus_tag="VIV63_04210"
     CDS             complement(3162..>3914)
                     /locus_tag="VIV63_04210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6123831.1"
                     /translation="KLFEEYAQAIARRPLSELTPEDREQIKENLVRVELIAQEAEKNG
                     VAKDPDVITRLDLARLELLQQASAQKYMKEHTPTEAEMRAEFEQQIASAPLIEYQARH
                     ILVSSEDVAQKIIAQLKSGSDFAGLAKRMSADKVSAAKGGELGWFGPRDTDPAFTEAV
                     ALLKKGEFTKTPVQTSAGWHVIQLQATRDRTPPAYDDVKDRLAKIVVAKAFKSHSDEM
                     LKTAKIEPALTTLPAAAPAPAPAAAPAAPAKP"
BASE COUNT          656 a         1321 c         1289 g          648 t
ORIGIN      
        1 tgtgacatcg tgaaacagat gtccgagcgc gtgcagttca tcttcatcac gcacaacaag
       61 gccaccatgg agctcgcctc gcaattgata ggcgtcacca tgaccgaacc cggctgctcg
      121 cgcctggtgt cggtcgacgt cgacgaggcg gtgcgcatgg cgacgggcta accaccagcg
      181 atgcccgagc tgcgcatcat cctgctcgtc gtcggcgtgt tgttcgtcgc cggcatcgcc
      241 gggttcgaat ggtggcgctc gcgcggctcc cgtccgctcg ccacgggcgt gcctcgtgac
      301 gacggcgcag ccgacgcgcc gccgcgtccc gccccggcac cgctgccgga tatcaacgtg
      361 gtacgcgatt cgcgcgttgc gatgtccgat tcactgcccg tgatcgaact cgcgagcacg
      421 tccgagtccg gaacgcgccg cgcgctcggc atctccatct ccgacgacgt cgcggtggac
      481 gtcgcccacg acagcggttc gcgcgaaccc gcgaagccgc gccccgaacc cacgcgcaac
      541 gaaccttaca tcggccagca cgacgagatt tccgaagtcc gcatcgtcga cgagcagccc
      601 gtgcgcggac cgcagctcgt gctctcgtgg cccaacgaga ccgaacggcg catcgtcacg
      661 ttgcgtgtca tcccgcgcgt cgatccgcgc ttccagggtc gcgcggtgcg acaggctttc
      721 agcgccgccg gcttctggca cggcccgctc gacatctacc accttcccga cgaaggcggc
      781 cgcgtggtgc tgagtgcggc cgcactcgca cagcccggca ccttcgatcc gtcgatcatg
      841 gattcgcagc gcttctccgg gctgaatctg ttcgcggtgc tgcccggccc cagacctgag
      901 cgcgagacct tcgacgaact ggtccacgcg gcgcgacagc tcgccgaaag actcgatgga
      961 acgctggccg atcagcacgg cgaggagctc actccgcaac gcatcgcgcg actgcgccag
     1021 gcgctcgaac cgccggcgac ctcgcgcgct tccggagaag cgtgaacccg gatggccgcg
     1081 cccaaaactg cagatagtca ggccgctcag cgcgccgcgg cgctgcgcga tgaaatcaac
     1141 ggccacgact accgctacta cgtgctcagc gagccgtcgg ttccggatgc cgaatacgac
     1201 cggctgatga aggaactgcg cgagatcgaa accgcgcatc cctctctcat caccccggac
     1261 tcgcccacgc agcgtgtctc cggtgtcgcc gccgcggaat tcggcgaagt cacccacacc
     1321 atccccatgc tgtcgctgga gaacgggttc tccgacgagg acctttccga cttcgatcgc
     1381 aaggtgcgcg agaggctggg cgtaccgggc cccatcgaat acgcggccga acccaaactc
     1441 gacggactcg cgatttcggt gatgtaccgg aacggcgaat tcctgcgcgc cgccacgcgt
     1501 ggtgacggcg tcacgggcga ggacgtgtcg gccaacgtgc gaacgatccg cgggttgccc
     1561 gcgcgcctgc gcggcaagcc gccggctctg ctcgaagtcc gcggtgaagt gttcctgccg
     1621 ttcgccggat tcgagaggat gaatcgcgat gccgcggcgc ggggcgacaa gttgtacgtg
     1681 aacccgcgta atgccgcatc gggcagcctg cgccagctcg atccgcgcat caccgcgtcg
     1741 cggccgctcg atctcttttt ctactcgctc ggcgtggtgg agggcggtgg cattcccgac
     1801 cgtcacagcg agctcgcggc gcagctcaac gcgtggggcc tgcgcacctg tccggaggcg
     1861 aagaaagtca ccggcatcga aggttgcctc gactactacc gcgacatcgg cgcccggcgc
     1921 gccaggctgc cgtaccagat cgacggcgtg gtctacaagg tagatagccg cgcggaccag
     1981 gagaagctcg gattcgtgtc gcgggcaccg cgctgggcgc tggcgcacaa atttcccgcc
     2041 gacgaagagc tcacgatcct cgaggacgtg atcttcaacg tcggccgcac gggcgcgctc
     2101 acgccggctg cgaagctcaa acccgtgttc gtgggtggcg tgaccgtgag caacgcgacg
     2161 ctgcacaaca tggacgaagt cgcgcgcaag ggattcatga tcggcgacac cgtggtcgtg
     2221 cgccgcgcag gcgacgtgat tcccgaggtg gcgcgctatc ttccggaaaa acgtccggag
     2281 aatgcgcggc ccatcgtaat gcccaccatc tgcccggtgt gtcagtcgcc ggtggtgcgg
     2341 ctcgaggacc aggccgtcta caagtgcacg ggcggcgtgc tcaagtgccg cgcgcagcgc
     2401 gccgaatgga tcatgcactt cgctgcccgg cgcgcgatgg acatcgaggg cctcggcgaa
     2461 aagctcatcg agcagctcgt gaacgacggg gcagtgagct caccggccga tctctacgat
     2521 ctggaggcga agtcactcgc cgagcgcgaa cgcatgggcg aaaaatccgc gcagaacgtc
     2581 gtcgacgcca tcacgaagag caagaagacc acgctgccgc ggttgctgtt cgcgcttggc
     2641 attccgcagg tgggcgaatc cacggcgctg gcgttggccc agcaattcgg cacggtcgaa
     2701 aagctcgaag gcgcgaccgc cgccgagatc gaggaaacgc ccgatgtcgg cccgatcgtc
     2761 tcgcgctccg tgttcgaatt cttccagagc gaactcgcga agtcggtggt cgaacgtttg
     2821 aagcgtagcg gcgtcacgta cacgcccatc gcggtcgaag cacgtgccag cctgccgctc
     2881 gcgggtctca ccatcgtgat caccggcacg ctcgcgggca tgcagcgcga cgcggcggaa
     2941 gacgcgcttc gagagctcgg cgccaaggtg tcgggaagcg tgtcgaagaa gaccagcttc
     3001 ctcgtggtgg gggccgacgc cgggtccaag ctggccaagg ccgaggcgct gggcgtgcgc
     3061 atcctcgatg aggccgcgct cgaacaaatc ctgaaaacaa aacagccgcc ggccccggtc
     3121 tgatgacggg cgccggcggc tgtagttaga accgctgcgg tttaaggctt cgcgggcgcg
     3181 gccggcgccg cggcaggagc gggggccgga gccgccgccg gcagcgtggt cagcgccggc
     3241 tcgatcttgg cggtcttcag catctcgtcc gagtgcgact tgaaggcctt ggccaccacg
     3301 atcttcgcca gccggtcctt cacatcgtcg taggcgggcg gagtgcggtc gcgcgtggcc
     3361 tgcagctgga tcacatgcca tccagccgaa gtctgaacgg gcgtcttcgt gaattcgcct
     3421 ttcttcagca acgccacggc ttcggtgaat gccggatccg tgtcgcgcgg gccgaaccag
     3481 ccgagctcac cgcccttggc tgctgagacc ttgtcggccg acatgcgctt ggccaggccg
     3541 gcgaaatcgc tgcccgactt gagctgcgcg atgatcttct gcgccacgtc ttcgctgctc
     3601 accaggatat gtcgcgcctg gtattcgatg agcggggcgg aggcgatctg ctgttcgaac
     3661 tcggcgcgca tctccgcctc ggtcggcgtg tgttccttca tgtacttctg cgcggaggcc
     3721 tgctggagca gttcgagccg ggccaggtcg aggcgcgtga tgacatcggg gtccttcgcc
     3781 acgccgttct tctcggcttc ctgcgcgatg agttcgacgc ggacgaggtt ttccttgatc
     3841 tgctcacggt cttccggggt cagctcgctc aggggccggc gggcgatggc ctgcgcgtat
     3901 tcctcgaaga gctt
//
LOCUS       DATDEU010000171         8743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1195775, whole genome shotgun sequence.
ACCESSION   DATDEU010000171 DATDEU010000000
VERSION     DATDEU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195775"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2330
                     /locus_tag="VIV63_04215"
     CDS             <1..2330
                     /locus_tag="VIV63_04215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6123832.1"
                     /translation="RRDEAEARSLPDDYIVSLAEDRGGSLWIGTKSGGLAKWNPRTWS
                     FGHVRASAEEGYSDRNITSFADDKLGRLWIGTFGSGINLLDRNSGRITPVRHVSGVRT
                     SLSDERVMALLEGNTGEVWVGTMGGGLNRFDPRTLKAEVYQHDPGVPTSLAAPGVMSL
                     LEVDKQLWVGTFGGGVSRFDARTRRFENLRPGPEDGLHLSSGRVTALARDRTGHVWIG
                     TDGGGLNVWDQKTRRLYYYKRGAKQLDTLSADTIYSILVDDTGGVWIGTRGGGLDRVL
                     NPAEAPHGLRFENLSEAQGLPNNTVYGLRADGTGNIWISTNFGLARLDPRTQDIQRFH
                     RLHGLQGEEFNFGAHYRDRSGKLFFGGSAGYNTFYPEVLEFNERPPRVVLTQFLKLNT
                     PGIVGVPEDRIERLELGHKEDVITLKFAALDFADPRANRYEYKLDGFDSDWVRADERR
                     AATYTNLPGGQYVFRVRASNSDGVWSTQDLALPIDVAPSPWLSPFAFLGYALLAVFML
                     LAVWYAQQRRIARAAMQRQELEQQVSDRTYELAERNRELEDANRRLEMASLSDPLTGL
                     ANRRYLMQQFPKLIADRKGSSGLAIMIIDLDALKPINDQHGHAAGDEVIVEIAKTLRQ
                     AIRPDDVLARWGGDEFVVVAHAQHVEHACMLAERVRERIAKMKCVLPRGAVVRTSCSI
                     GLTCLPFVPGNPESVSWEQAIKIADLALYRAKRGRNAWRGWFGTEHVATLNSVISAVE
                     SNVDGLIANGVIIEQSSTRGNDDTVNALRVLREAR"
     gene            2356..4041
                     /locus_tag="VIV63_04220"
     CDS             2356..4041
                     /locus_tag="VIV63_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY6123833.1"
                     /translation="MDSDIRDALARSLPDLALIVRRDGVILSCVGGLAVRGLDTQEEA
                     IPGRTLDTLWPESVASHVLQTVRRILKTRKPAQQRYQDGDLKLEMRFQVQGFDRVLVV
                     CRDLGEGVTANDSGLYPQGASAMLPHRDGFEKSLRMALDMAALREDTVGVALIHIGGY
                     VTYQRVFDSGLANRLLEAAAKVIADNISEARTSLPRVARVSDDVIGVVLTDIRSRADA
                     DVAVERIVKALQHPVDLDGQIFQLAPQAGVTLSGTDGDRAIELIERALTTLDSGRRQG
                     EDRLVTFYSDTLRLASLTRLDWSQELTQAIQKDELELHYQPRLLLSTREIVAVEAFLR
                     WPHKIRGLVPTSEFLPIAELSGLSVQLGRWVLRRACRDLAVLAERGYPNIRVSVNVGR
                     QYLSAESLAEDVTNSAREAGIEPSQLDLEITEQMLSTGRSGLAALHQLRGHGVRVLID
                     DFGTGYVSLGKLRSSPLDGIMIDRSFVEEVGHDHEARAVCRAAIALGLAFGLRVVAEG
                     IETEAQAQFLIAEQCHEGQGHLFCAATPLQNLLDKILPAGKESPASVQRASRR"
     gene            4260..6344
                     /locus_tag="VIV63_04225"
     CDS             4260..6344
                     /locus_tag="VIV63_04225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY6123834.1"
                     /translation="MSCSAATSGTPKAAVVADAGQSHSYIVEADSTDSAARAVKAAGG
                     RVESRLGVIDAVEATLTSSQHQAVLAMQGVKQVTPNAPVTTLAAANVRDNFEVGSFAN
                     NNGTHRWYGDWIEQNDNNSPYNGKISIGWPERGGNRMIVSSNGAIYRKAATPSTASIV
                     TLKLNYSRASLETGDYVAVQASANGGSTWTEVGRITGAGNDWGFTAKSFNITAYRGRN
                     TAIRFVSSMSDTFPNDYVDLDDVEITYNTTYGEGDPTPVDVNAKRLHASGIRGREIGV
                     AVIDTGYWKLNSLDLDSNGLGRVAAQYDAITNTVVSSWAPVSTDTTGHGTHVTSLIAS
                     SRARNNTDYFGVAPDARIISIKAFGEDGSSTYATVIRAIDYVVTNKNALAIRVLNLSL
                     GAQARSRYWDDPLNKAVMRAWASGIVVVVSAGNSGPLPQTVGVPGNVPYVITVGAMTD
                     NYTTNKNDDRLASFSSTGPTFEGFVKPDLVAPGGHAWGFMATYMKIAVDRPTYMNNGD
                     FFTMSGTSQAAALVSGVAALVINQNPSLTPDQVKCRIIASGKPAVKADGTLAYSVLQQ
                     GTGVVDAERAVYGTANNCANQGLNINADLGGTQHFMGSVRQRPDGTFTVATPSGPITD
                     QSYLWDQSYLWGQGYLWASSYLWPQGYLWMNAYLWPMGYDIPWVDGYPTEIGTSTSTS
                     SPMSINFWVTPE"
     gene            complement(6371..7054)
                     /locus_tag="VIV63_04230"
     CDS             complement(6371..7054)
                     /locus_tag="VIV63_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6123835.1"
                     /translation="MSGLELDHIMFFCSSGAPEADALLGRGLHEGPGNSHPGQGTVNR
                     RFFFRNMYLELLWIENFVEAQQPEVQRTGLWDRWVNRGNGGCPIGLVFRPGPDASAVP
                     FPSWTYVPKYFPAGFSIEVASDIPPNEPLLFYLPFARPALVENVAPMPGGVQIGAVAG
                     TTLHLPETSNLSPALNALVANRVVTIEPGRQVLVDIHHHSESREIIDLRPKLPLRFLP
                     ADRRSKAPH"
     gene            complement(7062..7901)
                     /locus_tag="VIV63_04235"
     CDS             complement(7062..7901)
                     /locus_tag="VIV63_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY6123836.1"
                     /translation="MGDNKVDLKNEGVHWDLGTSQSYGEYLQLDKLLDAQKPNSIEQD
                     EMLFIIVHQVSELWMRLFLHELDHVFECVRRDNLDPSFKMLARISRLQEELLGAWSVL
                     STLTPFEYSSFRNTLGRSSGFQSHQYRLLEFKLGNKNRDVIAVQKRDPAGFEKLERAL
                     NAPSIYDEVLRLLSRRGYGIPEELIERDFSEPYVASKAVTAAWLGVYHNAEKDWDLYE
                     MAERLTDLDQKFQLWRFHHLKTVERVIGYKPGTGGTGGVSYLAKALELKFFPELWHVR
                     TSM"
     gene            complement(7913..8659)
                     /locus_tag="VIV63_04240"
     CDS             complement(7913..8659)
                     /locus_tag="VIV63_04240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY6123837.1"
                     /translation="MDLLTPNEGTENGVGPLSGSPIIRASSRARRLSIRVYPDARVEV
                     VVPPRARPREVELFIAAHREWIDDKRAIALRNRPAPQPFPPAALDLRALPEVFRLHVA
                     GGTGPLRVTDAGENLLRITGAFTHGKLRTALRAWLMRMARQRLAPRVAAIAAATGVSF
                     SQVSVRRQRSRWGSCSARGTISLNCCLLFQRPEVVDYLIVHELMHVKHMNHSARFWQA
                     VERHCADWRALDRELVQGWRHVPRWVFAEE"
BASE COUNT         1632 a         2893 c         2712 g         1506 t
ORIGIN      
        1 atcgtcgcga cgaagccgaa gcccgatccc tgcccgacga ctacatcgtg tcgctggccg
       61 aggaccgcgg tggctcgttg tggataggca ccaagtccgg cggactcgcg aagtggaatc
      121 cgcgtacctg gtcgttcggc cacgtacgcg cgagcgctga agaaggttac agcgaccgca
      181 acatcacttc gttcgccgac gacaagctgg gccggctgtg gatcggcacc ttcggcagcg
      241 ggatcaacct gctcgaccgc aactccgggc gaatcacgcc tgtccgccac gtgagcggcg
      301 tgcgcacttc gctgagcgac gaacgcgtca tggcactgct cgagggcaac acgggtgaag
      361 tctgggtcgg cacgatgggc ggcggcctca accgcttcga tccgcggacg ctgaaggccg
      421 aggtctatca acacgatccc ggtgttccca cgtcgctggc cgctccgggc gtcatgagcc
      481 tgctcgaggt cgacaaacaa ctctgggtgg gcactttcgg gggcggcgtc tcccgcttcg
      541 acgcgcgtac tcgccgcttc gaaaatctgc ggcccggccc cgaagacggt ctgcacctgt
      601 ccagcggacg ggtgacggcg ctggcacgcg atcgcaccgg ccacgtctgg atcggtaccg
      661 acggcggcgg cctcaacgtc tgggaccaga agacccgccg gctctattac tacaagcgcg
      721 gcgccaagca gctcgacacg ctcagcgccg acactatcta ttcgatcctg gtcgacgaca
      781 ccggcggtgt gtggatcgga acgcgcggcg gcggcctcga ccgcgtgctc aatcccgccg
      841 aagcgccgca tggcctgcga ttcgagaatc tgtccgaggc gcagggactg cctaacaaca
      901 cggtatacgg cctgcgtgcc gacggcaccg ggaacatctg gatcagtacc aacttcggcc
      961 tcgcgcggct ggatcccagg acccaggaca tccagcgctt ccaccggctg cacggcctgc
     1021 agggcgagga attcaatttc ggcgcgcact accgcgaccg cagcggcaag ctcttcttcg
     1081 gcggctccgc cggctacaac acgttctacc cggaagtcct cgagttcaac gagcgtccgc
     1141 cgcgcgtggt gctcacgcag ttcctcaagc tcaatacgcc gggcatcgtc ggcgttcccg
     1201 aggatcgcat cgagcgcctt gagctcggtc acaaggaaga cgtcatcacg ctgaagttcg
     1261 cggcgctgga tttcgccgat ccccgcgcga accgctacga atacaaactc gacggcttcg
     1321 acagcgactg ggtgcgcgct gacgaacgcc gcgccgcgac ctacaccaac ctgcccggtg
     1381 gccaatacgt gttccgtgtc cgcgcgagca acagcgacgg cgtctggagc acgcaggacc
     1441 tcgcgctgcc gatcgacgtt gcaccgtcac cctggctgtc gcctttcgcc ttcctcggct
     1501 acgcattgct cgccgtgttc atgctgctcg ccgtctggta cgcgcagcag cgccgcatcg
     1561 cgcgcgccgc gatgcagaga caggaactcg aacagcaggt cagcgaccgc acgtacgaac
     1621 tggcggagcg caaccgcgaa ctcgaagacg cgaatcgccg tctcgagatg gcaagtctca
     1681 gcgacccgct caccggactc gcgaaccgcc gctatctcat gcagcagttc cccaagctga
     1741 tcgccgatcg caagggaagt tcgggtctcg cgatcatgat catcgatctc gatgcgctga
     1801 agcccatcaa cgatcagcac ggtcatgccg cgggcgacga agtcatcgtg gaaatcgcga
     1861 agacgttgcg ccaggccatc cgtcccgacg acgtgctcgc gcgctggggc ggtgacgaat
     1921 tcgtcgtcgt ggcccacgcg cagcatgtcg aacacgcctg catgctcgcc gagcgggtgc
     1981 gcgaaagaat cgccaagatg aaatgcgtgc tgccgcgcgg cgcggtggtg cgcaccagtt
     2041 gttcgatcgg cctcacgtgt ctgccgtttg taccgggaaa ccctgagtcg gtcagctggg
     2101 aacaggccat caagatcgcc gacctcgcgc tgtatcgcgc aaaacgcggc cgcaatgcct
     2161 ggcgcggctg gttcggcact gaacacgttg ctacattgaa ttccgtgatc tccgcggtcg
     2221 agtcgaatgt cgacgggctg atcgccaacg gtgtcatcat cgagcagagt tcgactcgcg
     2281 gtaatgacga cacggtgaat gcgctgcgcg tactgcgcga agcgcgttga ggccactcct
     2341 acacagcgga agatcatgga cagcgatatt cgagacgcac tggcccgatc cctccccgac
     2401 ctcgcgctga tcgtgcggcg cgacggcgtc atcctgtcct gcgtcggagg tctcgcggtt
     2461 cgcggtctcg acacccagga agaagcaatc cccggccgca cgctcgatac gctgtggccc
     2521 gaatccgtcg cctcgcacgt actgcagacg gttcgcagaa ttctcaagac ccgcaagccg
     2581 gcgcagcagc gctaccagga cggcgatctc aagctcgaga tgcgcttcca ggtgcagggc
     2641 ttcgaccgcg tgctggtcgt ctgccgcgat ctcggcgaag gcgtcaccgc caatgactcc
     2701 ggcctctatc cgcaaggcgc cagcgccatg ttgccgcacc gcgacggttt cgaaaaatcc
     2761 ctgcgcatgg cgctcgacat ggcggcgctg cgtgaagaca ccgtgggcgt cgcgctcatt
     2821 cacatcggtg gctacgtcac ctatcaacgc gtgttcgact ccgggcttgc caatcgcctg
     2881 ctcgaggccg ccgcgaaggt gatcgccgac aatatttccg aagcgcgcac cagcctgcca
     2941 cgcgtcgcgc gtgtgtccga cgacgtcatc ggcgtggtgc tcaccgacat ccgatcgcgc
     3001 gccgatgcgg acgtggccgt cgaacgcatc gtcaaggcgt tgcagcatcc cgtcgatctc
     3061 gacgggcaga tcttccagct cgcaccccag gcaggcgtga cgctgtccgg caccgacggc
     3121 gatcgcgcca tcgaactcat cgagcgcgca ctcacgacgc tcgactccgg ccggcgccag
     3181 ggtgaagacc ggctggtcac cttctattcc gacaccctgc ggcttgcgtc gctgacgcgc
     3241 ctcgactgga gccaggagct cacgcaggcc attcagaaag acgaactcga actccactac
     3301 cagccgcgct tgctgctgtc gacgcgcgag atcgtcgcgg tcgaggcgtt cctgcgctgg
     3361 cctcacaaga ttcgcggact cgtgcccacc tcggagttcc tgccgatcgc cgaactgtcg
     3421 gggctctccg tgcagctcgg ccgctgggtg ctgcgccgcg cctgccgcga tctggcggtg
     3481 ctcgccgagc gcggttaccc gaacatccgc gtgtcggtga acgtcggccg ccagtatctc
     3541 tccgccgaga gtctcgccga ggacgtcacc aactccgcgc gcgaagctgg catcgagccg
     3601 tcgcagctcg atctcgagat caccgaacag atgttgtcga ccggccgctc gggcctggcg
     3661 gcgctgcatc agctgcgcgg acatggcgtg cgcgtgctca tcgacgactt cggcaccggc
     3721 tacgtgtcgc tcggcaaact tcgcagttcg ccgctcgacg gaatcatgat cgaccgctcg
     3781 ttcgtcgagg aagtcggcca cgaccacgag gcccgcgccg tgtgccgcgc ggcgatcgcg
     3841 ctcgggctcg ctttcggttt gcgcgtggtg gccgaaggca tcgagaccga agcgcaggca
     3901 cagttcctga tcgccgaaca gtgccatgaa ggccagggcc atcttttctg tgcggcgacg
     3961 ccactgcaaa acctgttgga caaaatattg cccgcgggca aggaatcgcc agcttctgtt
     4021 caacgcgctt cccggcgctg acagtcgttg acatagcgca cggatttaac cgggtcttat
     4081 cagacgccgg tcacactttt cccgagatcc gcgtcacaca cgcacgctgc tgaattgccg
     4141 ttcccgcctc taatgtcgcc gtctgcaaaa gcagactggg gccaactgaa gaggcgttga
     4201 tgtacacaag ggtcgaaatt gctgctcgga tcgcaggagc gttagcgctt gcggggctgg
     4261 tgtcttgttc tgccgccacc tcgggcacgc ccaaggcggc ggtcgtcgcc gatgcgggcc
     4321 agtcgcacag ctacatcgtg gaagccgaca gcacggacag cgccgcgcgc gcggtgaagg
     4381 ccgccggtgg ccgcgtggag tcgcgcctgg gcgtcatcga tgccgtcgaa gccactctga
     4441 cgagttcgca gcaccaggcc gtgctcgcga tgcaaggcgt caagcaggtc acccccaacg
     4501 cgccggtgac cacgctggcc gccgccaacg tccgcgacaa tttcgaagtc ggttctttcg
     4561 ccaacaacaa cggcacgcat cgctggtacg gcgactggat cgagcagaac gacaacaatt
     4621 cgccgtacaa cggcaagatc tcgatcggct ggccggagcg cggtggcaac cgcatgatcg
     4681 tcagcagcaa tggcgccatc taccgcaagg ccgccacacc gtcgaccgcg agcatcgtga
     4741 cgctcaagct caactattcg cgcgccagcc tcgaaaccgg tgattacgtc gccgtgcagg
     4801 cgagcgccaa cggcggctcc acgtggaccg aagtcggccg tatcacaggc gccggcaacg
     4861 actggggctt cacggcgaag agtttcaaca tcacggctta tcgtggccgt aacaccgcga
     4921 tccgtttcgt gagctcgatg agcgacacgt tccccaacga ctacgtcgac ctcgacgacg
     4981 tcgagatcac ctacaacacc acctacggtg aaggcgatcc cacgcccgtg gacgtgaacg
     5041 ccaagaggct gcacgccagc ggtattcgcg gtcgcgagat cggcgtggcc gtgatcgata
     5101 ccggttactg gaaactcaat tcgctcgacc tcgactcgaa cgggctcggc cgcgtcgccg
     5161 cacagtacga tgcgatcacc aacaccgtgg tgagcagctg ggcgccggtg tcgaccgaca
     5221 ccaccggtca cggaacacac gtgaccagcc tcatcgcgag cagccgcgcc aggaacaaca
     5281 ccgactattt cggcgtcgcg cccgacgcgc gcatcatctc gatcaaggcc ttcggcgaag
     5341 acggttccag cacttacgcc accgtgatcc gcgccatcga ctacgtggtc accaacaaga
     5401 acgcgctggc catccgcgtc ctgaatttgt cgctaggggc ccaggcgcga tcgcgctatt
     5461 gggacgatcc gctcaataaa gccgtgatgc gcgcctgggc ttccggcatt gtcgtcgtcg
     5521 tttcagcggg caatagcgga ccgctgccgc agaccgttgg cgttccgggc aacgtgccct
     5581 acgtcatcac ggtcggcgcg atgaccgaca actatacgac taacaagaat gacgaccgtc
     5641 tggcgtcgtt ctcctcgacc ggcccgacct tcgaagggtt cgtgaagcca gacctggttg
     5701 cgcccggcgg tcacgcctgg ggcttcatgg ctacgtacat gaagatcgcg gtcgatcgtc
     5761 cgacctacat gaacaacggc gacttcttca ccatgtccgg cacgtcgcag gccgcggcgc
     5821 tggtatcggg tgtcgccgcg ctggtgatca accagaatcc gtcgctcacg cccgaccagg
     5881 tgaagtgccg catcatcgcc agcggaaaac ccgcggtgaa agccgatggc accctcgcct
     5941 acagcgtgtt gcagcagggc accggcgtgg tcgatgcgga acgcgccgtc tatggcacgg
     6001 ccaacaactg cgccaaccag ggtctcaaca tcaacgccga cctcggcggc acgcagcact
     6061 tcatgggcag cgtgcgccag cggccggatg gaacgttcac cgtcgccacg ccgagcggcc
     6121 ccatcaccga ccagagttac ctctgggacc agagctatct ttggggacag ggctacctgt
     6181 gggcgagcag ctatctctgg ccgcagggat atctctggat gaacgcctat ctctggccca
     6241 tgggctacga cattccgtgg gtggatggct atcccacgga gatcggcacg tcgacgagca
     6301 cgtcgagccc gatgtccatc aacttctggg tgacacccga gtagtgaggc ggtcccgaac
     6361 gggacctcga tcagtgggga gctttgctcc ttcgatcggc ggggagaaaa cgcaaaggaa
     6421 gtttcggtcg taagtcgatg atttccctgg actctgagtg atggtgaatg tcgaccagca
     6481 cctggcggcc cggctcaatc gtcaccaccc ggttcgcgac cagtgcattc aacgccggcg
     6541 acagattgct ggtttccggt agatgcagcg tggtgccggc aaccgcgcct atctgcacgc
     6601 cgcctggcat cggcgcgacg ttttcgacca gcgccggccg cgcgaagggt agatagaaca
     6661 acagcggctc gttcggcgga atatcgctcg cgacttcgat ggagaagccg gccggaaagt
     6721 atttcggaac ataggtccag gagggaaagg gcacggctga tgcgtcgggt cccggacgga
     6781 atacgagacc gatcgggcaa ccgccgtttc cgcggttcac ccatctgtcc cagagcccgg
     6841 tccgctgcac ttccggctgt tgtgcctcca cgaagttctc tatccagaga agctcgaggt
     6901 acatgttgcg gaagaagaag cgccggttga ccgtgccctg ccccggatgt gaattgcccg
     6961 ggccttcgtg cagtcctcga cccagcaatg catccgcttc cggcgcgccg gaagaacaga
     7021 agaacatgat gtgatcgagc tccaagccgc tcaccgccgc cttacatcga tgtgcgcacg
     7081 tgccagagct ccggaaagaa cttgagctcg agtgccttgg cgagatacga aacgccgccc
     7141 gtgccaccgg tgcccggctt gtagccgatg acccgttcca cggtcttcag gtgatgaaag
     7201 cgccagagct ggaatttctg gtcgagatcg gtgaggcgct cggccatttc gtagaggtcc
     7261 cagtctttct ccgcgttgtg atagacgccc agccacgcgg cggtgactgc tttcgaagcc
     7321 acgtacggct ccgagaaatc gcgctcgatg agttcctcgg gaatgccgta gccgcgccgt
     7381 gacagcaggc gcaacacttc gtcgtagata gacggcgcgt tgagcgcgcg ctcgagcttc
     7441 tcgaagccgg cggggtcgcg tttctggacc gcgatcacgt cgcggttctt gttgcccagt
     7501 ttgaattcga gcaggcggta ctggtgcgac tggaaacccg acgaacggcc cagcgtgttg
     7561 cggaacgagg agtactcgaa cggcgtgagt gtcgagagca cgctccaggc tccgagcagt
     7621 tcttcctgca gccgcgagat tcgcgccagc atcttgaacg acggatcgag gttgtcgcgg
     7681 cgcacgcatt cgaacacgtg gtcgagctcg tgcaggaaca gtcgcatcca cagttccgac
     7741 acctggtgga cgatgatgaa caacatctcg tcctgttcga tggagttcgg cttctgggcg
     7801 tcgagcagct tgtcgagctg caggtactcg ccataggact gcgaggtgcc gaggtcccag
     7861 tgcacgcctt cgttcttcag atctaccttg ttgtcgccca tttgccttgt agtcactctt
     7921 ccgcgaagac ccatcggggc acgtgacgcc atccctgcac gagctcgcga tcgagcgcgc
     7981 gccagtcggc gcaatgtcgt tccacggcct gccagaaccg cgccgaatgg ttcatgtgtt
     8041 tcacgtgcat gagctcgtgc acgatgaggt agtcgaccac ctcgggacgc tggaacagca
     8101 gacagcagtt gaggctgatg gtaccccgcg ccgagcaact accccagcgc gaacgctggc
     8161 ggcgcaccga aacctgcgaa aagctcacgc ccgtggccgc ggcgatcgcc gcgacccggg
     8221 gtgcaagccg ctggcgtgcc atgcgcatga gccaggcccg caaggcagtg cgcaacttgc
     8281 cgtgggtaaa cgcacccgtg atccggagca ggttctcgcc tgcatcagtg acccgcagcg
     8341 gacccgtgcc accggccacg tgcaggcgaa agacttccgg cagcgcgcgc agatcgagag
     8401 ccgccggcgg aaagggctgc ggcgcaggac gattgcgcag cgcgatggcg cgtttgtcgt
     8461 ctatccattc acgatgcgcg gcgatgaata gttcgacttc gcgcggccgc gcgcgtggcg
     8521 gcaccaccac ttcgacacgc gcgtccggat agacgcggat cgacagacgc cgtgcccgcg
     8581 aactcgcgcg aatgatggga gaaccgctga ggggtccgac cccattttcc gtgccttcgt
     8641 tgggggtcag cagatccaac tgaaaatggg gtctgacccc attcttgtcg tcgctcataa
     8701 gcgcgccgcc agcgtgacgc cttcgcgact cgccctttcc gca
//
LOCUS       DATDEU010000172         2638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1779784, whole genome shotgun sequence.
ACCESSION   DATDEU010000172 DATDEU010000000
VERSION     DATDEU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779784"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..2319)
                     /locus_tag="VIV63_04245"
     CDS             complement(301..2319)
                     /locus_tag="VIV63_04245"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY6123838.1"
                     /translation="MRITRAPLIAALLLCASALADESAGPAYVPYQPSGIYALGETAG
                     WNVTLPWNSPTVTYVIRKNNLAEIGRGTLMPGKPARIEAKLDEPGMVYVEVTENNPGA
                     KPKALGAAVAPERIAPAIPAPADFDAFWAAKIKALRKVPAKPELAVKPSDKDGVDFAI
                     LKMNHVEGKHVWGQVAKPSDPTHKKKYPGLVILQWASPPYPLQKSWVTDRAAEGWLAV
                     NIEPHDVMPDQPKEYYDALPAELKSYNTIETRNRDKNYFLYMYLADVRAVDYLASRPD
                     WDGKTLVVMGTSMGGQQSLCAAGLNPKVTHMLINVPAGADANGNAHGRKIGYPFWDVN
                     DPQVLKTAAYFDTVNCAARIKAPSLVSMGFIDTVTTPVSIWAAFNQIKAPKEAAPMID
                     SPHNHLATPEQSQPWTQRSAAWLSALVAGRSPFEPSDVATPRTDANSKLAHEQLLAKR
                     KAGQIDVYFMGDSITRRWGATDPQYQDLLANWRANFQGWNAANFGWGADKTQHMLWRL
                     QNGELDGVNPKVVVLMAGTNNVGRATPLGDASARASEVARGVAAIVREIRQRAPDATL
                     IITGITPRNDNVAVMPVIDEANAQIARLADGKKIRYVNINGQLATQDHQLLPGMANDG
                     LHLTVKAYQVWADALKPLFTEILGPPAAVDRAPPPTGDPSAPKPAQSP"
     gene            complement(2340..>2638)
                     /locus_tag="VIV63_04250"
     CDS             complement(2340..>2638)
                     /locus_tag="VIV63_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123839.1"
                     /translation="GLPTWGDGRLFVLGTEGYIELRKYINVASGLEGGNHLYIVDKKQ
                     ARYIDCKNVQLPFGPQFVADIVNRTQVAQDQEQALLAAELVLKAQTIATPPISA"
BASE COUNT          400 a          789 c          903 g          546 t
ORIGIN      
        1 gtcgttgggg ccttcgcgtg tttcgagctg cacggacagc tcgggaatgt ccttgtcgtc
       61 ttcacccgcg atgtaaagcg cgctgccgat tccgaacagg atgacgagat ggacgacggc
      121 ggagatggtg agcgccgtgc tcacgtcgcg atgggggaga aatgcggtcc aacaccttgc
      181 cgacgcccga ccgatggtga gcgctgcgtc tcgatacgcg ttcgcgtacg cgggcgtgga
      241 cacccgagac ctccgaaaat tctcccctaa ggccgatctt gctccgttcg cgcggtcagg
      301 tcacggactt tgcgcgggct tcggagcgct gggatcgccc gtcggcggcg gcgcacggtc
      361 aaccgccgcc ggtggtccca gaatctcggt gaacagcggt ttcagggcgt cggcccagac
      421 ctggtacgcc ttcacggtca ggtgcaggcc gtcattggcc atgccgggca gcaattggtg
      481 gtcttgcgtc gccaactgcc cgttgatgtt tacgtaacgt atcttcttgc catccgcgag
      541 ccgcgcgatt tgcgcgttcg cctcgtcgat gacgggcatg acggcgacgt tgtcattgcg
      601 cggcgtgatg ccggtgatga tcaacgtggc atcgggagcc cgctgccgga tttctctgac
      661 aatggcggcc acaccgcgcg cgacttccga agcccgtgcg ctggcatcgc cgagcggcgt
      721 cgcgcggccg acgttgttag taccggccat cagcacgacg accttgggat tgacgccgtc
      781 gagctcgccg ttctgcaggc gccacaacat gtgctgcgtc ttgtcggcgc cccagccgaa
      841 gttcgcggcg ttccagccct gaaaattggc gcgccagttg gccagcaggt cctggtactg
      901 ggggtcggtc gcgccccagc gccgcgtgat ggaatcgccc atgaaataga cgtcgatctg
      961 gccggccttg cgtttggcga gcagctgttc atgcgcgagt ttcgaattcg cgtcggtgcg
     1021 tggcgtcgcg acgtccgacg gttcgaacgg cgagcggccg gcgacgagtg cgctgagcca
     1081 ggcggcggag cgttgagtcc agggctgcga ttgctcgggc gtggcgagat ggttgtgcgg
     1141 cgagtcgatc atgggcgccg cttccttggg cgccttgatc tggttgaacg cggcccagat
     1201 gctgacgggc gtggtgacgg tatcgatgaa gcccatggat accagcgacg gcgccttgat
     1261 gcgggcggcg cagttcacgg tgtcgaaata ggccgcggtc ttcaggacct gcgggtcgtt
     1321 cacgtcccag aacgggtagc cgatcttgcg gccatgcgcg ttgccgttcg cgtccgcgcc
     1381 ggcgggcacg ttgatgagca tgtgcgtgac cttgggattg aggcccgcgg cgcacagact
     1441 ttgctggccg cccatgctcg tgcccatgac caccagcgtc ttgccgtccc agtcggggcg
     1501 gctggccagg tagtccacag cgcgtacgtc ggccaggtac atatataaga agtaattctt
     1561 gtcccggttg cgcgtttcga tcgtgttgta gctcttgagc tcggccggca gcgcgtcgta
     1621 gtactccttg ggttggtcgg gcatgacatc gtgcggttcg atgttcaccg ccagccagcc
     1681 ttcggcggcg cggtcggtca cccatgactt ctgcagcggg taaggcgggc tggcccattg
     1741 caggatgacg aggccgggat acttcttctt gtgagtcggg tcgctgggtt tggcgacctg
     1801 accccagacg tgtttgccct cgacgtggtt catcttgagg atggcgaaat ccacgccgtc
     1861 cttgtcactg ggtttgacgg cgagttccgg tttcgcggga accttgcgca gcgccttgat
     1921 cttcgcggcc cagaacgcgt cgaaatccgc gggcgcgggt atggcggggg cgattctttc
     1981 aggtgccact gccgcgccca gtgctttcgg cttggcgccg ggattgttct cggtgacttc
     2041 gacgtacacc atgccgggct catcgagttt cgcttcgatg cgtgcgggct tgccgggcat
     2101 gagcgtgccg cggccgatct cggcgagatt gttcttgcga atcacgtagg tgaccgtggg
     2161 cgagttccac ggcagcgtca cgttccagcc ggcggtttcg ccgagcgcgt agatgcccga
     2221 gggctggtag ggcacgtagg ccggtcccgc cgattcgtcg gccagtgccg aggcacaaag
     2281 aagcaatgcc gcgatcagcg gcgcgcgtgt gattctcatg gtccccctct tgcgaaaaat
     2341 caggcgctga tcggcggtgt cgcgatcgtc tgcgccttga gcacgagttc ggcggctaac
     2401 aaagcctgct cctggtcctg cgcgacctgc gtgcggttca cgatgtcggc gacgaactgc
     2461 ggaccgaagg gcagctgtac atttttgcag tcgatgtaac gcgcctgctt cttgtcgacg
     2521 atgtacaggt gattgccacc ttcgaggccc gaggcgacgt tgatgtactt gcgcagttcg
     2581 atgtaaccct cggtgccgag cacgaacagg cgaccatcac cccaggtcgg gagaccat
//
LOCUS       DATDEU010000173         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_320726, whole genome shotgun sequence.
ACCESSION   DATDEU010000173 DATDEU010000000
VERSION     DATDEU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320726"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VIV63_04255"
     CDS             complement(<1..494)
                     /locus_tag="VIV63_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEY6123840.1"
                     /translation="MELRFRILNVFTSGARLSGNPLCVVEDGSGLDPAAMQALARQFN
                     VSETTFILPSKRAAALVRIFTPAYEMPFAGHPTLGTAHVCRALGLGGNQLGLEMRAGI
                     IPVTSNGDFWTLRAAAATSRLVEVQPPELARLLGLDSSDIGFQPLWMKAGREQLVIPL
                     TSEAA"
     gene            complement(570..1370)
                     /locus_tag="VIV63_04260"
     CDS             complement(570..1370)
                     /locus_tag="VIV63_04260"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY6123841.1"
                     /translation="MQKTDSPKKVSLRSVFVSDVHLGSRDCRARELLQFLEGVETDYL
                     FLVGDIIDFWSLRRSFYWPEEHNDVVRAILTKAKEGTQVIYVPGNHDDNIREFCGSLF
                     GNLSIRRRYVHATADGREFLVMHGDEFDSAVKCSRWLARFGATAYEVMMRINRGVNRC
                     RRAFGLRHWSLATYLKLRLANAVRYVEAFEHAAAQAARSRNLAGIVCGHIHRPGLRQI
                     DGVLYCNDGDWVESCTALVESNSGQLSLWHAENLYERREQQELIEIAA"
     gene            complement(1469..1828)
                     /locus_tag="VIV63_04265"
     CDS             complement(1469..1828)
                     /locus_tag="VIV63_04265"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEY6123842.1"
                     /translation="MAYVDGFLIPVPKKNAAKYKKMSTQAGKVWKDHGALEYHECVAD
                     DVQYGKYTSFPQGVKLKKGEQVWFSWIVYKSRKDRDRVNAKAMKDPRLAHMMDPKAMP
                     FDGKRMVVGGFKMVVDL"
     gene            complement(1804..>2748)
                     /locus_tag="VIV63_04270"
     CDS             complement(1804..>2748)
                     /locus_tag="VIV63_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6123843.1"
                     /translation="DLAKFLSYAIFMGTSVAALSEMWGELQRASGAMERTIELLHSRP
                     SIVTPAVPTPIEVSRGAITFEHLAFNYPSRPDTRALDDFDLSIAAGETVAFVGPSGAG
                     KSTVFQLLLRFYDPGSGRILIDGLDIAAADPSAVRARIGLVPQETMIFGATARENIRY
                     GRPGASDADIEAAARAAAADEFIARLPEGYDTFLGEKGARLSGGQKQRIAIARAILKN
                     PPILLLDEATSSLDSESEQLVRDALEKLMRDRTTLVIAHRLATAINSDRIVVVDRGRI
                     VGIGRHEELLRDNALYARLAALQFGERGDSPWPMSMDS"
BASE COUNT          494 a          893 c          856 g          505 t
ORIGIN      
        1 gccgcttcgc tggtgagcgg tatcaccagc tgctcgcgac ctgccttcat ccacagcggc
       61 tgaaagccga tgtccgagga gtcgagccct aacaagcgag cgagttcggg cggctggact
      121 tcgaccagcc gtgaggtcgc cgcggcggcg cgcagcgtcc agaaatctcc attcgaggta
      181 accgggatga tgccggcgcg catttcgagc cccaactggt tgccgcccag cccgagcgcg
      241 cggcacacgt gcgcggtgcc gagcgtggga tggccggcga atggcatttc gtaggccggc
      301 gtgaatatgc gcaccagcgc cgcggcgcgc ttggagggca ggatgaacgt ggtttccgac
      361 acattgaact ggcgggccag cgcctgcatg gcggcgggat cgagcccgct gccgtcttcg
      421 accacgcaca gcggattgcc ggagagccgg gcgcctgacg tgaacacatt gaggatgcga
      481 aagcgaagtt ccatggcgcg ggattatttc acaatcccgg acccgtctaa cgctacgaac
      541 cccgaatccc tgacaactgt actgaagtgt caggcggcga tctcgatcaa ctcctgctgc
      601 tcccggcgtt catagaggtt ttccgcatgc cacaacgaaa gctgtccgct gttggattcc
      661 accagggccg tgcaactctc cacccagtcg ccgtcgttgc agtacagaac gccatcgatc
      721 tgtcgcaggc ccggacgatg gatatggccg cacacgatgc ccgccagatt gcggctgcgt
      781 gccgcctgcg ccgcggcgtg ctcgaacgct tcgacatacc gcactgcgtt ggccaggcgt
      841 agcttgagat aggtggcgag cgaccagtgc cgcagtccga acgcacggcg gcaacgattc
      901 acgccgcggt tgatgcgcat catgacttcg taggcggtgg ctccgaaccg cgccagccaa
      961 cggctgcact tcactgccga gtcgaactca tcgccgtgca tgacgaggaa ctcgcggcca
     1021 tccgcggtcg cgtgcacgta ccggcggcga atactgagat tgccgaacaa cgacccgcag
     1081 aattcacgga tgttgtcgtc gtgattgccg ggcacgtaga tgacctgcgt gccttccttc
     1141 gccttggtca ggatggcgcg gaccacgtcg ttgtgttctt ccggccagta gaacgaccgg
     1201 cgaaggctcc agaagtcgat gatgtcgccg acgagaaata gatagtcggt ttcgacgcct
     1261 tcgaggaact gcagcagctc tctggcgcgg cagtcgcgcg accccaggtg cacgtcggac
     1321 acgaacaccg aacgcagact gaccttcttc ggactgtccg tcttctgcat gcccacacct
     1381 ctatggttca aggagtgggg ccgagtctgc ccaagtcccg tttcatcttt atgactccgg
     1441 gacttacgag actgtcttaa cgctttgcct agaggtcgac caccatcttg aagccaccga
     1501 ccaccatgcg cttgccgtcg aacggcatgg ccttcggatc catcatgtgc gcgagccgcg
     1561 gatctttcat cgccttcgcg ttcacgcgat cgcgatcctt gcgcgacttg tagacgatcc
     1621 acgagaacca cacctgctcg cccttcttga gcttcacgcc ctgcgggaac gaggtgtact
     1681 tgccgtactg gacgtcgtcg gccacgcact cgtgatactc gagcgcgccg tggtccttcc
     1741 agaccttgcc tgcctgcgtc gacatcttct tgtacttggc ggcgttcttt ttcggaacgg
     1801 gaatcaggaa tccatcgaca taggccatgg gctgtctcct ctttcgccga attgtagcgc
     1861 cgcgagtcga gcgtacaacg cgttgtcgcg cagcagctcc tcgtggcggc cgattcccac
     1921 gatccgcccg cggtcgacga ccacgatgcg atccgaattg atggcggtgg ccagccggtg
     1981 cgcgatgacc agcgtcgtgc ggtcgcgcat gagcttctcg agcgcgtccc gaaccagctg
     2041 ctcgctctcg gaatcgagcg agctggtcgc ctcgtcgagc agcaggatcg gcggattctt
     2101 gagaatggcg cgcgcaatgg cgattcgctg cttctgccca ccggacagcc gcgcgccctt
     2161 ttcgccaagg aaggtgtcgt aaccctcggg caatctcgca atgaattcgt cggccgcggc
     2221 ggcgcgcgcc gcagcctcga tatccgcatc gctcgccccg ggccggccat aacggatgtt
     2281 ttcgcgcgcg gtggcgccga agatcatcgt ctcctgcggt acgaggccga tgcgggcgcg
     2341 caccgcggaa gggtccgccg ccgcgatatc caggccgtcg atgagaatcc gtcccgaccc
     2401 cgggtcgtaa aagcgcagca gcaactggaa cacggtgctc ttgcccgcgc ccgacggacc
     2461 gacgaaggcg accgtttcac cggccgcgat cgacagatcg aagtcgtcga gggcacgcgt
     2521 gtcgggcctc gacggatagt tgaacgccag gtgttcgaag gtgatcgcgc cgcgcgagac
     2581 ttcgatgggg gtcggcaccg ccggcgtcac gatggacggc ctcgagtgca acagctcgat
     2641 ggtgcgctcc atggcgcccg acgcgcgctg cagctcgccc cacatctccg acaacgccgc
     2701 gacggaggtg cccatgaaga tcgcgtaact caggaatttc gcgaggtc
//
LOCUS       DATDEU010000174         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_29977, whole genome shotgun sequence.
ACCESSION   DATDEU010000174 DATDEU010000000
VERSION     DATDEU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29977"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VIV63_04275"
     CDS             complement(<1..752)
                     /locus_tag="VIV63_04275"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEY6123844.1"
                     /translation="MKRALAICGTVVVTVFIAGTAAVGQTPAPATAAPAAQGTANKTP
                     PFKKEELEAIVAPIALYPDALLAQIFMASTYPLEIVEAARWSKANPSVTGDAVAGAVQ
                     SQTWDPSVKSLCAFPTVLEKMNEKIDWTQKLGDAFLAQQKEVMQAVQVLRGKAKDTGN
                     LKSTKEVTVKEEPAPAGSPAPQTIIIESPDPEVVYVPTYNTATVYGAWGYPYYPPYSY
                     YPPGYAYGAAFWSFTAGVIVGGAIWGNCNWGG"
     gene            942..>2156
                     /locus_tag="VIV63_04280"
     CDS             942..>2156
                     /locus_tag="VIV63_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY6123845.1"
                     /translation="MTGTAVTFGTFDWAIVALYFVANTAICIWCAMQKEKDTADYFLA
                     SRSAGWFLIGSSIFASNIGAEHLIGLAGSGADSGMAFAHWELHSYMVLILGWVFAPFY
                     LRASVFTTPEFLERRYTPGTRTFLSFIFLVSYILTKASVTIFAGAFAIQTILGYETLD
                     LPLLGVTDFFWFSAFFLVIITGVFVVLGGMKSVLWTEAMHVPVLLGGSAVLLFVGLGE
                     IGGLGALKAANPETIHLWRPLSTTPETQGFPGFLFDPSNTPWLGVLLCSPIIGLWYWC
                     TDQYIVQRVLTARGLKDARRGTIFAAYLKLAPVFIFLLPGMIAVALYKQGFPGFEGIG
                     ENPQSAFPVLVSNLLPVGLRGLVLAGMLSALMSALASLFNSTATLFTVDFYKRLKPQS
                     SEQHLVTVGRVAT"
BASE COUNT          331 a          691 c          671 g          463 t
ORIGIN      
        1 ttgccgcccc agttgcaatt gccccagatc gcgccgccga cgatcacgcc cgcggtgaag
       61 gaccagaacg ccgcgccgta tgcgtatccc ggcgggtaat aggagtacgg cgggtagtac
      121 ggataacccc aggcgccgta caccgtcgcg gtgttgtacg tgggcacgta aaccacttca
      181 ggatccggcg actcgatgat gatggtctgc ggcgcgggcg agcccgcggg cgccggttct
      241 tctttgaccg tcacttcctt ggtgctcttg agattgccgg tgtccttcgc cttgccgcgc
      301 agcacctgca cggcctgcat gacttctttc tgctgggcga ggaacgcgtc gccgagcttc
      361 tgcgtccagt cgatcttctc gttcatcttc tcgagcacgg tcgggaacgc gcacagcgat
      421 ttcacgctgg ggtcccaggt ctggctttgc acggcgccgg cgacggcgtc gcccgtgaca
      481 ctcgggttgg ccttggacca acgagcggct tcgacgattt ccagcgggta cgtcgatgcc
      541 atgaaaatct gcgcgagcag ggcgtcggga tacaacgcga tgggcgcgac gatcgcctcg
      601 agctcttcct tcttgaacgg tggagtcttg ttcgccgtgc cttgcgcggc aggcgcggcg
      661 gtcgccgggg cgggcgtctg gccgactgcg gccgttccgg cgatgaatac ggtgactacg
      721 acggttccgc agatcgctaa tgcgcgcttc attatcttgt acctcgcgct gtgtcccggg
      781 cccggtaagc gctccttgct taatagtggg gcagacacaa acgccttcgt catgggacga
      841 agtgcctaca aattgtggcc ggttggcgcg gcatatctca cggcgcttca ccatcagcat
      901 ttcgcacact aagctgcggg ccgggctgaa cgggggagat catgacggga accgcagtta
      961 cattcggcac gttcgattgg gccatcgtcg ccctgtactt cgtcgccaac acggcgatct
     1021 gcatctggtg tgcgatgcag aaagagaaag acacggccga ctacttcctg gccagccgca
     1081 gcgccggctg gttcctcatc ggatcgtcga tcttcgcgtc gaacatcggc gcggaacatc
     1141 tgatcgggct cgcgggttcg ggtgcggact ccggcatggc cttcgcacac tgggagctgc
     1201 attcctacat ggtgctgatc ctgggatggg tgttcgcccc gttctatctg cgcgcgagcg
     1261 tgttcaccac gccggagttc ctcgagcgcc gttacacgcc aggtacgcgt acgttcctct
     1321 cgttcatctt cctcgtgagc tacatcctca ccaaggcatc ggtgaccatc ttcgctggcg
     1381 cattcgccat ccagaccatc ctgggttacg agacgctcga tttgccgctg ctcggcgtca
     1441 ccgacttctt ctggttctcg gcgtttttcc tggtgatcat caccggcgtg ttcgtggtgc
     1501 tcggtggcat gaagtcggtg ctctggaccg aggccatgca cgtgcccgtg ctgctcggcg
     1561 gttccgcggt gttgttattc gtggggctcg gggaaatcgg tggactgggt gcgctcaagg
     1621 ccgccaatcc tgaaacgatt cacctgtggc ggccgctgtc gacgacgccc gagacccaag
     1681 gcttcccggg attcctgttc gaccccagca acacgccctg gctgggcgtc ttgctgtgct
     1741 caccgatcat cggactctgg tactggtgca ccgatcaata catcgtgcag cgcgtgctca
     1801 cggctcgcgg cctcaaggac gcgcgccgcg gcaccatctt cgcggcctac ctcaagctcg
     1861 cgccggtgtt catcttcctg ttgcccggca tgatcgccgt cgcgttgtac aagcagggct
     1921 tcccgggctt cgaaggaatc ggcgagaacc cgcagagcgc gttcccggtg ctcgtctcca
     1981 acctgttgcc cgtcgggctt cgcggactgg tgctcgccgg catgttgtcc gcgctcatga
     2041 gcgcgctcgc gtcgctgttc aactcgaccg cgacgctgtt caccgtggac ttctacaaac
     2101 ggttgaagcc gcaatcgagc gaacagcacc tggtgaccgt cggccgcgtc gccacc
//
LOCUS       DATDEU010000175         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1926331, whole genome shotgun sequence.
ACCESSION   DATDEU010000175 DATDEU010000000
VERSION     DATDEU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926331"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VIV63_04285"
     CDS             <1..700
                     /locus_tag="VIV63_04285"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123846.1"
                     /translation="SVWAEIESLDNAVAADVQYGMFYRASRLLRHTSYWLLRERAGDL
                     HIDNSVRELRPGVEQLIDSIDAVIGGEARALHDATLAGLAGSGVPERLARRVARLSLL
                     EPALDIVALARAEKLPVADVARVYFELAVLLGLDWLHGEIDRLVVDGSWQATARSNLR
                     DAAMRAHRELTHQVLNTRGPKRVGERVERWSEQRAEALAGWQRTLSEMRATGAADFAT
                     LTVGVEAVRGLSSG"
     gene            704..1498
                     /locus_tag="VIV63_04290"
     CDS             704..1498
                     /locus_tag="VIV63_04290"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6123847.1"
                     /translation="MRLALVITTYERPDALAAVLDGVRRQSVAPAEIVIADDGSGPAT
                     RELVTAFIARSPIPARLVSQQHEGFRVTRLRNLAIAATTADYLVLLDGDMLPHPKFIA
                     DHLRHARPGFYTQGVRVHADETLTRRLIADPATLPSFSSAGLGGLRRLYLLRSPALSS
                     LTRSLANGLIAVKSCNQGIWRDDLIRVNGFDEAIEGWGAEDKELCARLENAGVRRQTL
                     LFGGIACHLHHAPASRAALPANLAALERTRREHRVRSERGLDAHFE"
     gene            1538..>2288
                     /gene="gpmA"
                     /locus_tag="VIV63_04295"
     CDS             1538..>2288
                     /gene="gpmA"
                     /locus_tag="VIV63_04295"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY6123848.1"
                     /translation="MPGKLILVRHGQSVWNVENLFTGWHDVDLSDLGRIEAKSAGVEI
                     RKAGLVPDIAFTSVLKRAIRTLWSVLDEMDLMWLPVERSWRLNERHYGALQGLDKAQT
                     VEKHGEAQVKIWRRSYDIPPPPLELDDKRHPRFDARYAKVDPRVLPASESLKDTLARV
                     LPFWNDRIVAELLAGKNVLVVAHGNSLRAVVKMLDNVSEAEITELNIPTGVPLVYELD
                     AGLKPIKSGYLGDPEAIKAAAEAVKKQTEKKK"
BASE COUNT          390 a          770 c          754 g          374 t
ORIGIN      
        1 ctcggtgtgg gcggagatcg agtcactgga caacgccgtg gccgcggatg tgcagtacgg
       61 catgttctac cgcgccagcc ggttgctgcg tcacaccagc tactggctgc tgcgcgagcg
      121 ggccggcgac ctgcacatcg acaattccgt gcgcgaactg cgccccggcg tcgaacagct
      181 catcgacagc atcgacgcgg tgatcggcgg cgaagcgcgc gctctgcacg atgcgacgct
      241 ggccggactc gcgggcagcg gcgtgccgga gaggctggcg cgccgtgtcg cgcgcttgtc
      301 gttgctcgag cctgccctcg acatcgtggc gctggcgcgc gctgaaaagc tgccggtcgc
      361 ggacgtggcc cgcgtgtatt tcgaactcgc cgtgttgctt ggactcgact ggttgcacgg
      421 agaaatcgac cggctcgtgg tcgacggttc ctggcaggcc accgcgcgct ccaacctgcg
      481 tgatgccgcc atgcgcgcgc atcgcgagct cacgcaccag gtcttgaaca cgcgagggcc
      541 gaagcgcgtc ggcgaacgcg tcgaacgctg gagcgaacaa cgtgccgagg cgctcgccgg
      601 atggcaacgc acgctcagcg aaatgcgggc caccggcgcc gcggatttcg ccacgctcac
      661 cgtgggagtc gaagcggttc gcggtctgtc gagcggctga gggatgcgcc tcgcgctggt
      721 gatcaccacc tacgagcgcc ccgatgcatt ggctgccgtc ctcgacggcg tccggcgtca
      781 aagcgtggct ccggccgaaa tcgtcatcgc cgatgacggc tcgggcccgg ccacgcgcga
      841 gctggtcaca gccttcatcg cccgctcccc gatacccgcg cgcctggtgt cccagcagca
      901 cgaaggtttt cgcgtcacgc gacttcgcaa cctcgccatc gcggcaacca cggctgacta
      961 cctggtcctg ctcgacggcg acatgctgcc gcatcctaag ttcatcgccg accatctgcg
     1021 tcacgcccgg ccgggcttct atacgcaggg tgtccgtgtt catgccgatg agacgctcac
     1081 gcgacggctg atcgcagacc cagccacact gccgtcgttt tcgtcggccg gtctcggcgg
     1141 gctgcgccgc ctctatctac tgcgttcgcc cgcgctgtcg tcgctcacgc gttcgctcgc
     1201 caacggtctc atcgccgtga agagctgcaa ccagggcatc tggcgcgacg acctcattcg
     1261 cgtgaacggt ttcgacgagg cgatcgaggg ctggggtgcc gaggacaagg agctttgcgc
     1321 aaggctcgag aatgccggcg tgcgccggca gaccttgctg ttcggcggca tcgcctgcca
     1381 cctgcaccat gcaccggcgt cgcgcgccgc actgcccgcg aacctcgcgg cgctcgaacg
     1441 gactcggcgg gaacaccggg tgcgcagcga acgcggcctc gacgcgcatt tcgaatgacc
     1501 tgcgggtaat attgcgctcc cttctggagg agcgcgcatg cccggtaaat tgatcctggt
     1561 ccgacacggc cagagcgtct ggaacgtcga gaatcttttc accggttggc acgacgtcga
     1621 cctgtcggac ctgggccgca tcgaggcgaa gtcggccggc gtcgaaatca ggaaggccgg
     1681 cctcgtcccg gacatcgcgt tcacgtccgt actcaaacgc gcgatccgca cgttgtggag
     1741 cgtgctcgac gaaatggatc tcatgtggct gcccgtagaa cgcagctggc gcctgaacga
     1801 acgccactac ggcgcgctgc aggggctcga caaggcgcag accgtcgaga aacacggcga
     1861 ggcgcaggtc aagatctggc gccgcagcta cgacattccg ccgccgccgc tcgagctgga
     1921 cgacaagcgc catccgcgct tcgacgcgcg ctacgcgaag gtcgatccac gcgtactgcc
     1981 tgcttccgag tcgctcaagg acacgctcgc tcgcgtgctg ccgttctgga acgaccgcat
     2041 cgtggcggaa ctgctggccg gcaagaacgt gttggtggtc gcgcatggca actctttgcg
     2101 cgccgtggtg aagatgctcg acaacgtgag cgaagcggaa atcaccgagc tcaacattcc
     2161 gaccggcgtg ccgctggtgt acgaactgga tgccggcctc aagcccatca agagcggcta
     2221 tctcggcgat ccggaggcca tcaaggccgc ggccgaagcc gtgaagaagc agacggaaaa
     2281 gaagaagt
//
LOCUS       DATDEU010000176         2140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1051961, whole genome shotgun sequence.
ACCESSION   DATDEU010000176 DATDEU010000000
VERSION     DATDEU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051961"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VIV63_04300"
     CDS             complement(<1..366)
                     /locus_tag="VIV63_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY6123849.1"
                     /translation="MKTKFPAPFVVTRQALSRRRFLQGVGVAMSLPILDAMQSRSARA
                     ASSSVTADATPRRMLGICNNLGLRPDLFFPTGAGRAYEASAYLKLLEAHRNDFTVFSG
                     VSHPNVDGGHPSDISFLTAA"
     gene            complement(363..>2140)
                     /locus_tag="VIV63_04305"
     CDS             complement(363..>2140)
                     /locus_tag="VIV63_04305"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY6123850.1"
                     /translation="WRGRNNEPIGIYAQSPGLTRPVGVMDFTPEPTVGEVEVQLAGGE
                     SIRTDAMRLFRTRVNGSDEQYVNPLATKEGIPGYAVQWLEVTGPLEDKHTASGYKLLF
                     GDLPMRRLGAGEKGGVPLEIVAPNTIPPVGGPTGAQVFGAGGPGAFPRSPISNVAVEV
                     ISKAPQRDAERLLRAFLETTYRRPVEKSEMQRFLGLFKRELELGSGFARSMLTAYTGA
                     LVSPGFVFVEEQPGKLDDRALATRLSLFLWNSTPDSALRARAERGELHKPQVLRKETE
                     RLLNDPKSRRFVDAFTDYWLDLRKIDDTSPSATIYNDYELDEPLKLAALDETQLFFAE
                     LLRADLPARNIVASDFTYLNERLANHYGIQGVAGAKMRRVALPPDSERGGLMTMASVL
                     KLTANGTTTSPVLRGHWITERILGIETRPPPPSVKAVEPDIRGAVTIRQQLAKHRDNP
                     SCASCHIKMDPPGFALESFDVMGARRDRYRAVAENVKPEPGYGMNGQAFAFHYGLPVD
                     SAGELPDGHAFRDIKEFKRLVLRDEVAIARNLARQLVVFSTGAPVRFSDRGELEQILQ
                     RASARQYGVRTLVEEIVQSELFQTK"
BASE COUNT          378 a          666 c          713 g          383 t
ORIGIN      
        1 tgcggccgtg aggaaactga tgtccgacgg atgcccgccg tccacattcg gatgcgagac
       61 gccgctgaag accgtgaagt cgttgcggtg tgcctccagc agctttagat aggcggaagc
      121 ctcgtatgcg cgacccgcgc cggtcgggaa gaacagatcg ggacgcaagc ccaggttgtt
      181 gcagatgccg agcatccggc ggggcgtggc atcggcggta acggacgaag acgcggcgcg
      241 cgcggacctg gactgcatcg catcgagaat cggcagcgac atcgcgacgc ccacaccttg
      301 caggaagcgc cggcgcgaga gcgcctggcg ggtgacaacg aagggagcgg gaaatttcgt
      361 tttcatttgg tttgaaacaa ctcgctttga acgatttcct cgaccagggt gcgcacgccg
      421 tactggcgtg cactcgcgcg ctgcaagatc tgctcgagtt cgccgcggtc ggagaagcgc
      481 accggtgcac ccgtcgagaa gaccactaac tgcctcgcga ggttgcgggc gatggccact
      541 tcgtctctca agacgaggcg cttgaactct tttatatcgc ggaaggcgtg gccgtccggt
      601 aactcaccgg cggagtccac gggaagccca tagtgaaagg cgaacgcctg accgttcatc
      661 ccataaccgg gttcgggctt cacgttttcg gcgaccgcgc gataacgatc gcggcgcgcc
      721 cccatcacat cgaaactctc cagcgcgaag cccggtggat ccatcttgat gtggcacgac
      781 gcgcagcttg gattgtcgcg atgcttggcg agctgctggc ggatggtcac cgcgccgcgg
      841 atatccggtt cgacggcctt cacgctcggc ggcggcggac gagtctcgat gccgagaatc
      901 cgctcggtga tccagtggcc gcgcagtacc ggcgaggtgg tcgtgccgtt ggccgtgagc
      961 ttgagcacac tggccatcgt catcaggccg ccgcgctcgc tgtcgggcgg aagcgcgacg
     1021 cgtcgcatct tcgcgcccgc cacgccctgg atgccgtagt ggttggcgag acgttcgttc
     1081 agataggtga aatccgaggc cacgatgttt cgcgccggca ggtccgctcg cagcagctcg
     1141 gcgaagaaca actgcgtctc gtccagcgcg gccagtttga gcggctcgtc cagctcgtag
     1201 tcgttgtaga tagtggcgga cggcgaggtg tcgtcgatct tgcgcaggtc gagccagtag
     1261 tcggtgaacg cgtccacgaa acggcgcgac ttcggatcgt tgagcagccg ctcggtctcc
     1321 ttgcgcaaca cttgaggctt gtgcaactcg ccgcgttcgg cgcgggcgcg cagcgcggaa
     1381 tctggcgtgg agttccagag aaacagcgac agacgagtgg cgagggcccg gtcgtcgagc
     1441 ttgcccggct gctcctcgac gaacacaaaa ccgggcgaca cgagcgcgcc cgtgtaagcg
     1501 gtgagcattg aacgtgcgaa gccggagccg agttcgagct cgcgtttgaa cagcccgagg
     1561 aaccgttgca tttcggattt ctcgacgggc cgccggtagg tagtctcgag aaatgcgcgc
     1621 aacaggcgct cggcgtcccg ttgcggggct ttggaaatta cttccaccgc cacgttgctg
     1681 atgggcgaac gcggaaacgc acctggccca cctgcgccaa agacttgagc gccggtggga
     1741 ccgcccaccg gtggaatggt attcggcgca acgatttcca gcggtacacc gcctttctca
     1801 cccgcgccaa gccgccgcat cggcaggtcg ccgaacagca gcttgtagcc actcgcggtg
     1861 tgtttgtctt ccagcggccc cgtgacttcg agccattgca ccgcgtagcc gggaatgccc
     1921 tccttcgtcg cgagcgggtt cacgtactgc tcgtcggagc cattgacgcg cgtgcggaag
     1981 agccgcatcg cgtcggtacg gatgctttcg ccgccggcga gttgcacttc gacttcgccc
     2041 accgtgggct ccggcgtgaa atccatgacg ccgacgggcc gcgtcaagcc cgggctctgc
     2101 gcataaattc cgatgggctc gttgttgcgc ccgcgccaga
//
LOCUS       DATDEU010000177         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1926800, whole genome shotgun sequence.
ACCESSION   DATDEU010000177 DATDEU010000000
VERSION     DATDEU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926800"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(731..1243)
                     /locus_tag="VIV63_04310"
     CDS             complement(731..1243)
                     /locus_tag="VIV63_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6123851.1"
                     /translation="MKAISSHLTRVAVSYALGFAAAATANSADVVRHPLPGGSTFPIA
                     RAVEVPAGKTIIFHSGTTPAPLDAKAEAGTAAYWGDTKTQALSTFARIKESLDAMKLD
                     FGNVVAMTVYLVGDPAKGGRMDFEGFMAAYSQFFGTKEQPNLPARSTVQVAGLVAPGM
                     LVEIEVQLVK"
     gene            complement(1256..1873)
                     /locus_tag="VIV63_04315"
     CDS             complement(1256..1873)
                     /locus_tag="VIV63_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6123852.1"
                     /translation="MKLRHSIAAKATLSFVLALSLGNASAGNDFDYCLLCHGANANGN
                     YGIRAPKLSGMEPWYLARQLENFANGARGTPADDAPGHEMRPVGLRLKQEGVIDAAVQ
                     FIVKLESRRPAPTVSGDVAHGKQLYENCASCHGARGEGNPALQSPALAARSDWYLVTQ
                     LANYQKGLRGADERDTYGAQMRAIVATLPDEKAITDVVAYINTLK"
BASE COUNT          344 a          616 c          660 g          413 t
ORIGIN      
        1 gtcggttcgg cgggtgccga gcacgtagat ctcctccaag ggctttgctt cctctgactc
       61 ggcgttcgcg tcgcccgctt cttgtgcctg tacctgcgag cccgcgagtg cgagcaagat
      121 gcccgcaggg attgcggata gacgggggaa tttttgtccg gatttcagtt tcataaactt
      181 gcgacctccc aacagtagtt tgattttttc actcgcgacg ttcccctcgt gtccgtcgcg
      241 gtttcgatgg tctagccgtg ccttctcgta gtgcgcaggg cgttgggtaa gtcattgcaa
      301 taagccggcc atcacgccgc tgaagtcgtt gcgaatacgc acttgcgtac cgacttcgac
      361 gccgacgagc gccgcagcaa gagatgtttt ctcttggctt tttcgggctt tattgcgcgg
      421 agcaacgacg accggaggag cgggaaaaag ttgttaggac gactcaagat gaacacagag
      481 tgttcaacct tgactgcact gtgtcaccgc atcgcgcacg aatccgctcc gttatcgcgc
      541 ctgcgcagat tctcgatgaa attttttcac gctgggccgt gttcggcgca tgcgcactcc
      601 tggtgcatgc gaagaagggc atccgggcgg cgggactgtg cgcgacgtgt cgcagaacgc
      661 actgcgcact ttgttagggc gcaaaaaaag ggcgagcagc ggtgctcgcc ctttcccgtt
      721 gaagagaaaa ctatttgacg agctgaactt cgatctcgac cagcatgccc ggcgccacga
      781 ggcccgcgac ctgcacggtg gagcgcgccg gcaggttcgg ctgttccttg gtgccgaaga
      841 actgcgagta cgcggccatg aaaccttcga agtccatgcg gccgcccttg gccggatcgc
      901 cgaccaggta gacggtcatc gccacgacat tgccgaagtc gagcttcatg gcatcgagcg
      961 attccttgat gcgcgcgaac gtggagagcg cctgggtctt cgtgtcgccc cagtaagccg
     1021 cggtgccggc ctcggccttg gcgtcgagcg gtgcgggcgt ggtgccgctg tggaagatga
     1081 tggtcttgcc ggctggcact tcgaccgcgc gcgcgatcgg gaacgtgctg ccgccaggca
     1141 acggatggcg gacaacgtcg gcggaattcg ccgtggctgc ggccgcgaag ccgagtgcat
     1201 agctcaccgc gacgcgcgtg agatgggaac tgatcgcttt catgaagcct cctgactact
     1261 tgagcgtgtt gatgtaggcc acgacgtccg tgattgcctt ttcatcgggt aaggttgcga
     1321 cgatagcgcg catctgcgcg ccataggtgt cgcgttcatc ggcgccgcgc aggcccttct
     1381 gatagttcgc gagctgcgtc acgagatacc agtccgatcg cgccgccagc gccggcgatt
     1441 gcagcgcggg attgccttcg cccctggcgc catgacagct cgcgcagttc tcgtagagtt
     1501 gcttgccgtg cgccacgtcg cccgacaccg taggcgcggg ccgtcgggac tcgagcttga
     1561 cgatgaactg cacggcggcg tcgatgacgc cttcctgctt cagcctcagg cccacgggcc
     1621 tcatctcgtg gcccggcgcg tcatcggccg gcgtgccgcg cgcaccgttc gcgaaattct
     1681 cgagctggcg cgcgagatac caaggctcca ttccggagag cttcggggca cggatgccgt
     1741 aattgccgtt ggcgttggcg ccgtggcaga gcaggcagta atcgaagtcg ttgccagcgc
     1801 tggcgttgcc gagcgagagc gccagcacga aactcaaggt ggcttttgcg gcgatcgaat
     1861 ggcgaagctt catgccgcca ccgccccgcc ggcgcgttcg cgggcgtcga tgtcggcgat
     1921 ggcgaaatgc gccgaggcga tggcgccttc ctgccaaccg gggtgataac tcacctggtc
     1981 gcccatcagg tagtggctgc cgaccggcgt ctgcagcgtc ttgaaccaac gtt
//
LOCUS       DATDEU010000178         3983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1635239, whole genome shotgun sequence.
ACCESSION   DATDEU010000178 DATDEU010000000
VERSION     DATDEU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635239"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VIV63_04320"
     CDS             <1..557
                     /locus_tag="VIV63_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123853.1"
                     /translation="RTDTHAVSIAMPEGEPSDGMRLGHLLIDEFTVLHAATTAHYITN
                     DQEKAFQLLDGFRSRLASSSITGLEDEKKLIDSLHSRIAFLSGHSSEGAQVAPYVKLW
                     GRWTVERINGGIDFKRGEVLEFTPDNSLLRVDEDEEEYQSNEKQIYLPESDLVFHYRI
                     KGDKLTLHHRGANVWVRLRRDTEG"
     gene            complement(481..1083)
                     /locus_tag="VIV63_04325"
     CDS             complement(481..1083)
                     /locus_tag="VIV63_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123854.1"
                     /translation="MRATSIGSILLATLIGVGAAFWLNAKEARKPWPKATPPPPPSPI
                     YMTSTEVAPNEAEIADMARDHNRRLEMEIGSALRQKDAARREAAFTFLLPELVQVDPD
                     RVVAMVANLTPGEARDTLRSEVSRQWIVKDPDAAIRWMKSLPEGDRRASATTAIESIA
                     AHSPGEARKLAAEFGVNPRCRVSDGPRHWRHGDEESACRP"
     gene            complement(1183..1935)
                     /locus_tag="VIV63_04330"
     CDS             complement(1183..1935)
                     /locus_tag="VIV63_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123855.1"
                     /translation="MKRTHLLAISAVLSAVAIGTAYLFGTDEAPAQRAVPESRIGVAR
                     SPDTVVTETVASTDEKRDAGEARVRQIALGIERALVARDLKQRETAFAFLLPELIELQ
                     PDRVIALVARQEPGETRDALREEVVRQWIVRDRNAAIEWMGTLEDEAERKASATLAMR
                     TLATTAPAEAIEVADQFGVGRDDGSLEHMVQIWATEKPEEAMRWIDSQPAADPRTAQL
                     RARIERVRAQFAAGTYDTGGPNRTPSDLSDDM"
     gene            complement(1998..3896)
                     /locus_tag="VIV63_04335"
     CDS             complement(1998..3896)
                     /locus_tag="VIV63_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY6123856.1"
                     /translation="MTIRTAVLGRSLSIAFVLGAAAFRAGPAAAQMAAGKDKFVGNVT
                     GNSVPSNFNTYWNQITPENATKWGSVEGTRNSMNWAGADAAYNHAQTNGYKFKFHTFV
                     WGSQYPGWLTGLSQTEQRAEIEQWIQAACTRYPNTWAIDVVNEPIKTAMPFKAALGGD
                     GATGWDWVITSFQLARQYCPNAKLLINEYGTENDPPVRNTYKTIINLLKARGLIDGIG
                     IQAHYFNIDNMNRAAVTTMLNDYATLALDVWVSELDIVGPGGDAGQLAKYQDVFPGFW
                     EHASVKGITIWGYIVGQTWRDGTGLVTTGGQERPAMTWLKGYVSGTPGGDTQAPSVPA
                     GLTSTATTSSSVTLAWTASTDNVGVTGYQVLRAPGASGGTFAQVGTPTSNSFVNTGLT
                     ANTTYRYQVRAVDAAGNASAVSNTVTVTTQTGGGGDTQPPSIPANLTAPSTTTSSISL
                     AWTASTDNVQVTGYQILRAPGTSGGTFAQIATSTTNSYVNTGLTANTAFRYQVRAVDA
                     AGNASAVSNTVSATTQSGGTGGGCTVAATVQTQWQSGYVIEPARVTNTGTATINSWTV
                     TFTLPAGHAVTGSWNAVLTVSGQTVTARNASYNGTVGAGGNTAFGFQVSRPNGNTQLP
                     SGYVCATP"
BASE COUNT          647 a         1310 c         1249 g          777 t
ORIGIN      
        1 cgcgcacgga cactcatgcc gtctccatcg cgatgcccga aggcgagccc agcgacggca
       61 tgaggctcgg acacctgctc atcgacgaat tcaccgtact gcacgcggcg acgaccgcgc
      121 actacatcac caatgatcag gaaaaagcct ttcagttgct cgacggcttc aggagccgcc
      181 tcgcgtcgag ctccatcacg gggctggagg acgagaagaa gctcatcgac tccctgcact
      241 cgcgcatcgc atttctgtcg ggtcacagca gcgaaggcgc gcaggtcgcg ccgtatgtga
      301 agctctgggg ccgctggacc gtggaacgca tcaacggcgg aatagatttc aagcgaggcg
      361 aagtcctgga gttcacgccg gacaacagcc tgttacgagt cgacgaggat gaagaggagt
      421 accagtccaa cgaaaagcag atctacctgc ccgagtcgga cctcgtgttt cactaccgca
      481 tcaagggcga caagctgact cttcatcacc gtggcgccaa tgtctgggtc cgtctgagac
      541 gcgacaccga gggttgacgc cgaactcggc cgccagcttc ctggcttcac ccgggctatg
      601 cgccgcgatc gattcgatcg cggtcgtggc gctggcccgg cgatcccctt ccggcaggga
      661 cttcatccat cgaatcgctg cgtccggatc tttcacgatc cactggcggc tgacttcgct
      721 gcgcaacgta tcgcgcgctt cgcccggcgt gaggttcgcg accatggcga cgacacgatc
      781 gggatcgacc tgcaccagct ccggcagcag gaatgtgaaa gccgcctctc ggcgcgccgc
      841 atccttctga cggagcgcgc tgccgatttc catttcgagc cgccggttgt ggtcccgcgc
      901 catgtcggcg atctccgctt cgttgggcgc gacctccgtc gaggtcatgt agataggtga
      961 cggcggtggt ggcggcgtgg ccttgggcca tggcttgcgc gcttccttcg cattcagcca
     1021 gaacgcggcg cccacgccga tcagcgtggc cagaaggatc gaaccgattg aagtagcgcg
     1081 cataaaaaaa gacgggcagc gtttggtcgc tgcccgcaag tttactcagg cccggagagg
     1141 tggtcgttgg ttgccgtgcc gtccgtgcct gacggggtat cgctacatgt cgtcagataa
     1201 gtccgatggt gtcctgttgg gaccccccgt gtcatacgta cctgccgcaa attgggctcg
     1261 cacccgctcg atacgcgcgc gcagctgcgc ggtgcgtgga tccgcggccg gttgactgtc
     1321 gatccagcgc atcgcttcct ccggcttctc ggtggcccag atctgcacca tgtgctcgag
     1381 cgagccgtca tcgcggccca cgccgaactg gtcggccacc tcgatggctt cggccggcgc
     1441 cgttgtcgca agtgttcgca ttgcaagcgt cgcactcgct ttgcgctccg cttcgtcttc
     1501 gagtgtgccc atccattcga tcgcggcgtt tcgatcgcgg acgatccact ggcgcaccac
     1561 ttcctcgcgc agcgcatcgc gcgtttcacc tggctcctgg cgcgctacca gcgcgatcac
     1621 gcggtcgggc tgtagttcga tgagctcggg cagtagaaat gcaaacgcgg tttcgcgttg
     1681 tttgaggtcg cgtgcgacca gcgcacgttc gattccaaga gcgatctgtc gaacacgcgc
     1741 ctcgccggca tcgcgctttt catccgtcga agccacagtc tctgtaacca ccgtgtcagg
     1801 cgagcgcgca acaccgatgc gcgattccgg caccgcccgc tgcgcgggcg cttcgtccgt
     1861 gccaaacaaa tacgccgtgc cgatcgccac cgcggacaac acggcagaga tggccaggag
     1921 gtgagttcgc ttcatgtttc gcgaagcgcg ggcagcgttg tcagcgctgc ccgccactta
     1981 catgcgttcc tgcaatctca aggcgtcgcg cacacgtatc cgctcggcaa ctgcgtgttg
     2041 ccgttcgggc ggctcacctg gaagccgaag gcggtgttgc cgccggcgcc aaccgtgccg
     2101 ttgtatgacg cgttgcgcgc cgtcaccgtc tgtccgctga cggtgagcac cgcgttccag
     2161 gatcccgtca ccgcatgtcc cgccggcaac gtgaacgtca cagtccagct gttgatcgtc
     2221 gccgttcccg tgttcgtcac gcgcgcgggt tcgatcacgt agcccgactg ccactgcgtc
     2281 tgcaccgtgg ccgcaaccgt gcaacctcca ccggttccac cactctgcgt cgtcgcgctg
     2341 acggtgttcg acaccgccga tgcattcccc gcggcatcga cggcgcggac ctggtagcgg
     2401 aacgccgtgt tcgccgtcag gccggtgttg acgtacgagt tggtcgtcga cgtggcgatc
     2461 tgcgcgaacg tcccaccact cgtgcccggc gcgcgcagga tctgataacc cgtcacctgc
     2521 acgttgtccg tcgatgcggt ccacgcgagc gagatcgaac tcgtggtcgt cgacggagcg
     2581 gtcaggttgg cgggaatgct gggcggctgg gtatcgcctc cacctccggt ctgagtcgtc
     2641 accgtgaccg tgttcgacac tgcggacgca ttgcccgccg catcgacggc tcgaacctgg
     2701 tagcgatagg tcgtgttcgc ggtgagaccg gtgttcacga acgagttcga cgtgggcgtt
     2761 cccacctgcg cgaacgtacc gccactcgca ccgggcgctc gcagcacctg gtagcccgtg
     2821 acgcccacgt tgtcggtgga tgcggtccac gccagcgtga ccgaactgct ggtcgtcgca
     2881 gtcgacgtca gtcctgcggg cacgctgggc gcctgcgtat cgccgcccgg agttccggag
     2941 acgtagcctt tgagccaggt catggcgggt cgttcctgac ctcccgtggt caccagcccc
     3001 gtgccgtcgc gccaggtctg cccgacgatg tatccccaga tcgtgatgcc cttgacggag
     3061 gcgtgctccc agaatccggg aaacacgtcc tggtacttcg cgagctgacc ggcatcgccg
     3121 ccgggcccga cgatgtcgag ttccgacacc cacacgtcga gcgccagcgt ggcgtagtcg
     3181 ttcaacatcg tcgtgaccgc ggcgcggttc atgttgtcga tgttgaagta gtgcgcctgg
     3241 atgccgatgc cgtcgatgag tccgcgtgcc ttgagcaggt tgatgatggt cttgtaggta
     3301 tttcggaccg gtggatcgtt ctccgtgccg tactcgttga tgagtagttt ggcgttcgga
     3361 caatactgcc gcgcgagctg gaacgaggtg atgacccaat cccagcccgt ggcaccatcg
     3421 ccgccgagcg cggccttgaa cggcatcgcc gtcttgatcg gctcgttgac cacgtcgatc
     3481 gcccaggtgt tgggataacg ggtgcaagcg gcctggatcc actgctcgat ctccgcgcgc
     3541 tgctcggtct gcgacaggcc cgtgagccag cccggatact gcgagcccca cacgaacgtg
     3601 tggaacttga acttgtagcc gttcgtctgc gcgtggttgt acgccgcgtc ggcgcccgcc
     3661 cagttcatgc tgttgcgtgt gccctcgaca gaaccccact tcgtggcgtt ctcgggcgtg
     3721 atctggttcc agtaggtgtt gaagttggac ggcacgctgt tgcccgtgac gttgccgacg
     3781 aacttgtcct tgcccgcggc catctgcgcc gcggccggcc cggccctgaa cgcggctgcg
     3841 cccaaaacga aggcaataga aagcgatcgg ccgagaacgg ccgtacgaat ggtcattgga
     3901 tgtccccctc gtgaaacgag tagtcgaaaa gtcccttttt gcgcccgctc ctgtgcgtcg
     3961 tcccggacgc gggccgacca tac
//
LOCUS       DATDEU010000179         4015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1927386, whole genome shotgun sequence.
ACCESSION   DATDEU010000179 DATDEU010000000
VERSION     DATDEU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927386"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VIV63_04340"
     CDS             complement(<1..285)
                     /locus_tag="VIV63_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123857.1"
                     /translation="MLGAILVILTLIAGAAYVAKVGTGFTLLFIGAIVFMAYKRLPLI
                     AFTVVFTLLLAGYTYIAPPAGIWKGFLWVLLAALWLFNLRPLRKAIISRPF"
     gene            complement(356..1270)
                     /locus_tag="VIV63_04345"
     CDS             complement(356..1270)
                     /locus_tag="VIV63_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6123858.1"
                     /translation="MNANDSPTLEVADLPLILALSRERTLAGAAERLDVDLSTVFRRL
                     NTLEKRLRVRLFDRSARGYQLTPAGERAASAAERVETELLALDRDISGRDQQLSGVLR
                     VTASETLSHAVLPRLFARFQEIHPRIQIVLTIDNRVLDLGRREADVALRVRRPTDPAL
                     FGRRLTGIAWAFYGPAGDGPNLRRDGGSFKFAHHSVIGWDEPSRIVVGDWLGANVPAD
                     RIGYRSNSLVNQLKAVRAGIGIALLPCYLADAEDGVRRISGVLPDLASELWIVTHQDL
                     KNTARIRAFLGVIGDAIAAERRSFEGQT"
     gene            1371..1808
                     /locus_tag="VIV63_04350"
     CDS             1371..1808
                     /locus_tag="VIV63_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6123859.1"
                     /translation="MSALLERGDATGAALLRVTLGVMFLAHSVVLKLFTFGLAGTAGY
                     FASIGLPGGLAYAVFAAEAIGGALLLANVATGWVSLALIPVLAGAFWVHAGNGWVFSA
                     AGGGWEYPLFLIVASVVVALQAFAARARTVSTPAVLGAVARQA"
     gene            1819..2580
                     /locus_tag="VIV63_04355"
     CDS             1819..2580
                     /locus_tag="VIV63_04355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6123860.1"
                     /translation="MSNAGLFATVAREYANFRPGYPDEMFEWLARVSPSRAAVWDCGC
                     GSGQASVALAAHFSKVFATDVAPEQIGAARPHERVSYSVASAERSGLAEHSVDLVTVA
                     QALHWFDVTAFYGEAARVARPGALLAVWNYPRPQFVDAELDRRFFTFYSQVVGPYWPA
                     ERRHIESGYRTLPFPFEEIETPEFAVELDWDIDHVAGYVSSWSATARYKAALGADPLP
                     ILRESLGGAWPAHGAQVRIRMPIGLRVGKLARVTS"
     gene            complement(2577..3554)
                     /locus_tag="VIV63_04360"
     CDS             complement(2577..3554)
                     /locus_tag="VIV63_04360"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY6123861.1"
                     /translation="MKPAVNVAITGAAGQIGYALAFRVASGQMLGADTPVNLHLLEVT
                     PALPQLAGVVMELNDCAFPTLNKIVATDDPRVAFKDCHAALLVGARPRGPGMERKDLL
                     MANAQIFSAQGKAMNDVADRNIRVLVVGNPANTNAMIARANAKDLNPRNFTAMTRLDH
                     NRAVSQLAEKTGSHVTRIRKMLIWGNHSSTQYPDLNICTVDGKPAHSLVDRAWYEETF
                     IPTVQQRGAAIIKARGASSAASAASAAIDHMRDWHLGTAADNSISMAVPSDGSYGIAE
                     GVVYSFPVTTANGDYKIVQGLAIDEFSRKRMDATDKELREERDGVRELI"
     gene            complement(3620..>4015)
                     /locus_tag="VIV63_04365"
     CDS             complement(3620..>4015)
                     /locus_tag="VIV63_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123862.1"
                     /translation="KVFSGSRWTVPVALAATLLLAFTVVFQAGMPARKAPTPEVTVQN
                     VAQETVAAPPARSAVVEAASRERSVAGTSMDAPVMAHAGDVPAWRRDAKSWQAEIQRL
                     RDAGDNAAAEAELAEFKRQQRAYAVAPDR"
BASE COUNT          660 a         1252 c         1387 g          716 t
ORIGIN      
        1 gaacggacgc gagatgatcg ccttgcgcag cggacgcagg ttgaacagcc acaacgcggc
       61 gagcagcacc cacaggaaac ctttccagat gccggcgggc ggtgcgatgt aggtgtagcc
      121 ggcaagcagc agcgtgaaca ccacggtgaa ggcgatgagc ggcagacgct tgtacgccat
      181 gaacacgatg gcgccgatga acagcagcgt gaagcccgtg cccaccttgg ccacgtaagc
      241 ggcgccggcg atcagggtta ggatgactaa tattgcgccg agcatagtga agcccctcga
      301 ttcgcgatgg cgaaactata gtcgtttccg catcgcggcg tgcagcgcgc cggccttatg
      361 tctgcccttc gaagctgcgg cgttcggcgg cgatggcgtc gccgatgaca cccaggaaag
      421 cgcggatccg cgcggtgttc ttgaggtcct ggtgcgtcac gatccacaac tcgctcgcga
      481 ggtccggcag gacaccggaa atgcggcgca cgccgtcttc ggcatcggcg agataacagg
      541 gcagcagcgc gatgcctatt ccggcgcgca cggccttgag ctgattcacg agactgttgc
      601 tgcgatagcc gatgcgatcc gcgggaacgt tggcgccgag ccagtcgccc acgacgatac
      661 gcgagggttc atcccagccg atgacggaat ggtgcgcgaa cttgaacgag ccgccatcgc
      721 gacgcagatt ggggccatcg ccggcgggtc catagaaagc ccaggcaatt ccggtgaggc
      781 gccggccaaa caaggcggga tcggtgggac ggcgcacgcg cagcgccacg tcggcttcgc
      841 gcctcccgag atcgagtacg cggttgtcga tggtgagcac gatctggatt cgcggatgga
      901 tctcctggaa gcgcgcaaac agacgcggca gtacggcatg tgacagcgtc tcggaggccg
      961 tcacgcgcag cacaccggac agttgctgat cgcggccgct gatgtcccgg tcgagagcga
     1021 gcagttccgt ctcgacacgc tcggcggcgc tcgcggcgcg ttctccggca ggggtgagtt
     1081 ggtagccgcg cgccgagcga tcgaacaggc gtacacgcag ccgcttctcc agcgtattga
     1141 ggcggcggaa tacggtggac aggtcgacat cgaggcgctc ggcagcgccg gccagcgttc
     1201 tctcgcgcga caacgcgagg atcagcggca gatccgctac ttcgagcgtc ggggagtcgt
     1261 ttgcattcat gcaaacgaga tttgccatac ggccaatgag tttgcaacgg cgccaaccta
     1321 ggatctgctc catcgcaatg tcgcgtattc aactgccctg ggagattgtc atgtccgcac
     1381 tgttagagag aggcgacgcc acgggcgccg cactgctgcg cgtaacgctc ggcgtcatgt
     1441 tcctggctca cagcgtcgtg ctgaagctgt tcacgtttgg cctggcgggc accgctggat
     1501 acttcgcttc catcgggttg ccgggcggcc tcgcgtatgc cgtgttcgcg gccgaagcca
     1561 tcggtggcgc gctgctgctg gccaatgtcg ccacgggctg ggtgtcgctc gcgttgatcc
     1621 cggtgctggc cggagcgttc tgggtccacg ccggcaatgg ctgggtgttc agcgccgccg
     1681 gtggcggctg ggaatatccg ctgttcctga tcgtggcgtc ggtggtggtg gcgctgcagg
     1741 cgttcgccgc acgtgccagg acagtcagca caccggccgt ccttggggcg gtggcccgtc
     1801 aggcgtaaca atagcggcat gtcgaatgcc ggattgtttg caaccgtcgc gcgcgagtac
     1861 gcgaacttca ggcccggata tccggacgag atgttcgagt ggctcgcgcg cgtttctccg
     1921 tctcgcgccg cggtctggga ttgcgggtgc ggcagcggtc aggccagcgt cgcgctcgct
     1981 gcacattttt cgaaggtgtt cgccaccgac gtggcgcccg agcagatagg cgcggccagg
     2041 ccccatgagc gcgtctcgta ctcggtggcg tccgccgaac gcagcgggct tgccgagcat
     2101 tccgtggatc tggtgaccgt cgcgcaggcg ctgcactggt tcgacgtgac ggccttctac
     2161 ggtgaggccg cacgcgttgc acgtcccgga gcgctgctgg ccgtctggaa ctaccccagg
     2221 ccgcaattcg tggatgcgga actcgaccgg cgtttcttca cgttctattc gcaggtggtc
     2281 gggccgtatt ggcccgcgga acgccggcac atcgaatcgg gttaccggac gctgccgttc
     2341 ccgttcgaag agatcgaaac gccggaattc gcggtcgaac tcgactggga catcgaccac
     2401 gtcgccggat acgtgagcag ctggtcggcc accgcccgtt acaaggcagc actgggcgcg
     2461 gacccgctac cgatcttgcg cgagtccctg ggcggcgcat ggccggcaca cggcgcccag
     2521 gtccggatcc gcatgcccat cggcctgcgc gtcgggaaac tcgcgagggt gactagttag
     2581 atcagctcgc ggacgccgtc gcgttcttcg cgcagttcct tgtcggtggc gtccatgcgc
     2641 ttgcgcgaga attcgtcgat ggcgagcccc tggacgatct tgtagtcgcc attggcagtg
     2701 gttaccggga aggaatagac cacgccttcc gcgatgccgt agctgccgtc cgacggcacg
     2761 gccatgctga tggagttgtc ggccgcggtg ccgagatgcc agtcacgcat gtggtcgatg
     2821 gcggccgagg cggccgaagc ggccgaggag gcgccacgtg ccttgatgat ggccgcgccg
     2881 cgctgctgca cggtggggat gaaagtttcc tcgtaccacg cgcggtcgac cagcgagtgc
     2941 gccggtttgc cgtcgacggt gcagatgttg agatccggat actgcgtcga ggagtggttg
     3001 ccccagatca gcatcttgcg gatcctggtg acgtggctgc cggtcttttc ggccaactgc
     3061 gagacggcgc ggttgtgatc gaggcgcgtc atcgcggtga agttgcgcgg gttcaggtcc
     3121 ttcgcattgg cgcgcgcgat catcgcgttc gtgttggcgg gattgcccac gaccagcacg
     3181 cggatgttgc gatcggcgac gtcattcatg gccttgccct gggccgagaa gatctgcgca
     3241 ttggccatca gcaggtcttt gcgttccatg ccgggtccgc gcggacgcgc gccgaccagc
     3301 agggcggcat ggcaatcctt gaatgcgacg cgcggatcgt cggtggcgac gatcttgttg
     3361 agtgtcggaa acgcgcaatc gttgagctcc atcaccacac ccgcgagctg cggcagggcc
     3421 ggcgtgacct cgagcaggtg caggttgacg ggcgtgtccg cccctaacat ctgtccggaa
     3481 gcgacgcgga aggccagggc atagccgatc tgaccggcgg caccggtgat ggcgacattg
     3541 actgcgggtt tcatctgtac tccggacggc cgaaaaaacg ggccggaatc ttaacagggt
     3601 agcgcttcgg cgggcgcctt cagcggtctg gcgccaccgc gtaggcgcgc tgctggcgct
     3661 tgaactccgc gagttcggct tccgcggcag cgttgtcacc cgcgtcgcgc aatcgctgga
     3721 tctcggcctg ccagctcttg gcatcgcgtc gccaggccgg aacatcgccc gcgtgggcca
     3781 tgacgggcgc atccatcgag gtccctgcca cactgcgttc gcgactcgcg gcttcgacca
     3841 cggcactgcg ggcaggcggc gcggccaccg tctcctgggc cacgttctgc acggtgactt
     3901 cgggcgtggg cgccttcctg gccggcatgc cagcctggaa aacgaccgta aaggcgagca
     3961 acagcgtcgc cgccagcgcc accggcaccg tccagcgcga accgctgaac acttt
//
LOCUS       DATDEU010000180         5361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1635810, whole genome shotgun sequence.
ACCESSION   DATDEU010000180 DATDEU010000000
VERSION     DATDEU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635810"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VIV63_04370"
     CDS             complement(<1..725)
                     /locus_tag="VIV63_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY6123863.1"
                     /translation="MNVLIVDDEPLARAGMRLLLDEESAITSIAEARNGAEAVETVKA
                     RRPDLLLLDVQMPGMDGFGVLKELGAEHMPPVIFVTAHDKYAIQAFEVNAIDYLLKPV
                     TRERFQQALARVRERVSTQGLDNQHVLSLLQQLSAPPKYLARVALRSSGRISFVNIDD
                     VLYAQAAENYVQLHLKAARHLLHVPIATLEASLDPQMFLRIHRSLIVNVRHIQELETG
                     PHGEFIVVLHGGARLQSSRSYHE"
     gene            complement(722..1837)
                     /locus_tag="VIV63_04375"
     CDS             complement(722..1837)
                     /locus_tag="VIV63_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6123864.1"
                     /translation="MNARKSRTWIFYFLAWTLVGLIYFAQNVTRRFYWDDPNPWQDLR
                     YWSVNIYLAALLTPLIVWAGRRWPLERQNLPRLIALHFGLSIAWETLRLLLEGAFHLT
                     WNEFWPIQPPINVKSEFTLLFIFGFHTGVVTYWVVLSIQTAIRNYARFQEREQAALRS
                     DLRASQLETQVAQARLGALKAQLQPHFLFNTLNAIVVLVRQQKGQQAEETLARFSDLL
                     RAVLADMDAQEVTLARELEYLRLYLSIEQLRFSDRLRVEFDVEPDLLDAAVPHMALQP
                     LVENSIRHGVGQLATPGVISIRAARVGDALHVVVKDDGPGFDAHGAGGGLGLGLTNTR
                     ARLKQLYGESSELRTETGPAGGAVVTMILPFHFVEAS"
     gene            complement(1857..2327)
                     /locus_tag="VIV63_04380"
     CDS             complement(1857..2327)
                     /locus_tag="VIV63_04380"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY6123865.1"
                     /translation="MKVRLSGQTAAAMIICLAAAVAFAAPAGQVFYPDTYREWRVAKF
                     RFESKGFLRYHYANPQALESWGKFRDGSIIVDEKVHAKLGDDGTWREEAIAHVAVMRK
                     DAAAHADTGGWYFNFFTANDTSTGLTPEQAKARCFDACHKAQEPRDFVFSDPRR"
     gene            complement(2427..3629)
                     /locus_tag="VIV63_04385"
     CDS             complement(2427..3629)
                     /locus_tag="VIV63_04385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY6123866.1"
                     /translation="MTSSASITTLPLLNADELAHSQRVTEHIRAFMQQHGGVIGFDAW
                     MRLALYAPGLGYYSAGATKLGSNGDFVTAPEIGSLFSRCLARQCADVLQVTGGDILEL
                     GAGSGRMAADVLTELAAMDSLPDRYSILEVSADLAERQRARIAELPGELSARVQWLDR
                     WPERVMRGVVLANEVLDAMPVERFILRGRPGGIDVRALGVALAGVGFEWRETSPSPEL
                     MHAVADIVEALPMPLPDGYVSEVCLAFQPWMASLAAQLEAGVALLVDYGLPRAHLYHP
                     DRAAGTLRCHFRHRAHSDPFINVGLQDITAWVDFTRVAEAASSAGLEVLGFASQAGFL
                     IGAGIESLLSTEMQLAGDDTRQQARLAGEARRLLLPGEMGEIFKVIALGRGYESPLTG
                     FSTQTLAL"
     gene            3641..4396
                     /locus_tag="VIV63_04390"
     CDS             3641..4396
                     /locus_tag="VIV63_04390"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEY6123867.1"
                     /translation="MKSSDGSLTGKTVLITGGARRVGAAIARMLHGAGANLVIHYRKS
                     ATEAASLADDLNAARANSTVIFQADLLDLGKLPALVEFAVRRFGALDVLVNNASTFYP
                     TKIGEITPQAWDDLMGSNLKAPLFLSQAAAPALKKSGGLILNIVDIHALRPLRDYTVY
                     CSAKAGLHMLTRSLAKELGPEIRVNGISPGPVMWPEHGGDEASRAKIIQRTILQKMGT
                     PEDIARTALFFAASAPFITGQILAVDGGRSVAW"
     gene            complement(4397..4909)
                     /gene="folK"
                     /locus_tag="VIV63_04395"
     CDS             complement(4397..4909)
                     /gene="folK"
                     /locus_tag="VIV63_04395"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY6123868.1"
                     /translation="MNLLKPGHVRVFVAAGSNLEPEKNLVRACAEIKHAWHDAVFSRA
                     YRNVAVGFDGPDFINLVLGFTTSQPLDPVIAHLRAIETRCGRPRFAPKWASRTMDLDV
                     LLFGDRVEKTADYTLPRPDLLKRPYMLGPMAEIAAEVMHPTAHKTIGQLWEEFDRDGH
                     QMTPVELPAG"
     gene            complement(4915..>5361)
                     /gene="folB"
                     /locus_tag="VIV63_04400"
     CDS             complement(4915..>5361)
                     /gene="folB"
                     /locus_tag="VIV63_04400"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY6123869.1"
                     /translation="LDVLLFGDRVEKTADYTLPSSASTPDKGDRIFLHGLAVECIIGF
                     IEWERRVKQTIVLDVEMPVDCTRAAASDDVADTLDYKRVAKRLISFVEGSEFKLVETL
                     AHRTALLVLQEFGIEWVRLSINKPGAIRGSKDVGVAVLRTRADLPK"
BASE COUNT          966 a         1768 c         1714 g          913 t
ORIGIN      
        1 ttctcgtggt agctgcggct cgactgcagc cgcgcgccgc cgtgcaggac cacgatgaac
       61 tcgccgtgcg gtcccgtctc gagctcctgg atgtgccgca cgttcacgat cagcgaacga
      121 tgaatccgca ggaacatctg cggatcgagt gacgcttcca gcgtcgcgat gggcacgtgc
      181 agcaggtggc gcgcggcctt cagatgcagc tgcacgtagt tctccgccgc ctgcgcgtac
      241 aacacgtcat cgatgttgac gaacgagatc cttccggacg atcgcagcgc gacgcgcgcc
      301 aggtacttcg gcggcgccga cagctgctgc agcagcgaca acacgtgctg gttgtcgaga
      361 ccctgcgtgc tcacgcgttc acgcacgcgc gccagcgcct gctgaaaacg ctcgcgcgtc
      421 accggcttca gcagatagtc gatggcattc acttcgaaag cctggatggc gtacttgtcg
      481 tgcgcggtca cgaagatcac cggcggcatg tgctccgcgc cgagttcctt gagcacgccg
      541 aacccgtcca tgcccggcat ctgcacgtcg agcagcagca gatccggccg ccgcgccttc
      601 acggtttcga ccgcttcggc gccattgcgc gcctcggcga tcgaggtgat cgcggattct
      661 tcgtccagca gcagtcgcat gccggcgcgc gccagcggct cgtcgtcgac gatcagtaca
      721 ttcatgacgc ttccacgaaa tgaaacggca ggatcatggt gaccaccgcg ccgcccgcgg
      781 gcccggtctc ggtgcgcagt tcggaagatt ccccgtagag ctgtttgagg cgggctcgcg
      841 tgttcgtgag cccgaggccc aggcccccgc ctgcgccgtg tgcatcgaat cccgggccgt
      901 catctttgac gacgacatgc agtgcatcgc ccacgcgcgc cgcgcgaatg ctgatgacac
      961 cgggcgtcgc gagctggccc acgccgtgac gaatggagtt ttccaccagc ggctgcagcg
     1021 ccatgtgagg caccgccgca tcgagcaggt cgggctccac gtcgaactcg acgcgcagcc
     1081 tgtccgagaa tcgcagctgc tcgatggata gatagaggcg gagatattcc agctcgcgtg
     1141 caagcgtgac ttcctgcgca tccatgtcgg cgagcaccgc gcgcagcagg tcgctgaagc
     1201 gcgccagcgt ttcctcggct tgctgaccct tctgctgacg cacgagcacc acgatggcgt
     1261 tcagcgtgtt gaagaggaaa tgcggctgca actgcgcctt caatgcgccg aggcgcgcct
     1321 gcgccacctg cgtctcgagc tgcgaggcgc gcaggtccga tcgcagcgct gcctgctcgc
     1381 gctcctggaa tcgcgcgtag ttgcggatgg cggtctgaat gctgagcacc acccagtatg
     1441 tgaccacgcc ggtgtgaaag ccgaagatga acagcagcgt gaattcactc ttgacgttga
     1501 tcggtggctg gatcggccag aactcgttcc acgtcaggtg gaatgcgccc tccagcaaca
     1561 accgcaacgt ttcccacgcg atgctcagac cgaagtgcag cgcgatgagg cgcggcaggt
     1621 tctgccgctc gaggggccag cggcggccgg cccagacgat gagcggcgtc agcaacgccg
     1681 ctagatagat gttgaccgac cagtagcgca ggtcctgcca cgggttgggg tcgtcccagt
     1741 agaaacgtcg cgtgacgttc tgcgcgaaat agatgagacc gaccagcgtc caggcaagga
     1801 agtagaaaat ccaggtcctg gatttgcgcg cattcatggg acgaagagct taactctcag
     1861 cgacgcgggt cgctgaacac gaagtcgcgg ggttcctgcg ccttgtggca ggcatcgaaa
     1921 catcgtgcct tggcctgttc cggggtcaat ccggtggaag tgtcattcgc ggtgaagaag
     1981 ttgaagtacc agccaccggt atccgcgtgt gcggccgcgt ccttgcgcat gacggcgacg
     2041 tgcgcaatgg cctcttcccg ccaggtgccg tcgtccccga gtttcgcgtg gaccttctcg
     2101 tcgacgatga tcgagccgtc gcgaaacttg ccccagcttt cgagcgcctg cggattcgcg
     2161 tagtggtagc gcaggaaacc tttcgattcg aaccggaact tggccacccg ccactcgcga
     2221 taagtgtccg ggtagaagac ctggccggca ggcgccgcga aggcaaccgc cgccgcgagg
     2281 cagatgatca ttgccgcagc ggtttgtccg gacaatcgca ctttcatgga caggagatac
     2341 ccgatatcgc gcgacgccgg gaaaggttgt ttgaccaact ggccgttttc gttgaccggc
     2401 gactggaact aattcggccc agcccgctac agggccagcg tttgcgtgct gaatcctgtt
     2461 aggggcgatt cgtacccccg cccgagcgcg atgaccttga agatctcgcc catctctccc
     2521 gggagcagca gccgccgcgc ctcaccggcg agccgcgcct gttgacgcgt gtcatcgccg
     2581 gccaactgca tctcggtgga aagcagcgac tcgatgccgg cgccgatgag aaaccccgcc
     2641 tggctcgcga agcccagcac ctcgagtccg gcgctgctgg cggcttcggc cacgcgcgtg
     2701 aaatcgaccc acgcggtgat gtcctgcaat cccacgttga tgaaaggatc gctgtgcgcg
     2761 cggtggcgga agtggcagcg cagcgtgccc gcggcgcgat ccgggtgata caggtgcgcg
     2821 cgcggcaggc catagtcgac gagcagcgcc acgcctgctt cgagctgcgc ggcgagactg
     2881 gccatccacg gctgaaacgc caggcacacc tcggacacgt acccatcggg caagggcatg
     2941 ggcagcgctt cgacgatgtc cgcgaccgcg tgcatgagtt cggggctcgg cgacgtctcg
     3001 cgccattcga agccaacacc cgcgagcgcg acgcccagcg cacgcacgtc gatgccacca
     3061 ggccgcccgc gcaggatgaa ccgctccacc ggcatcgcgt cgagcacctc gttggccagc
     3121 accacgccgc gcatcacgcg ctcgggccag cggtctaacc actgcacgcg cgccgacagc
     3181 tcgccgggca gctcggcaat ccgcgcgcgc tgacgctccg cgaggtccgc gctcacttcg
     3241 agaatggaat agcggtcggg cagagaatcc atggcggcga gttcggtgag cacgtcggcg
     3301 gccatgcggc ccgatcccgc accgagctcc aggatgtcgc caccggtcac ctgcaacacg
     3361 tcggcgcact gccgcgcgag acagcgcgag aacaggctgc ctatctccgg cgcggtcacg
     3421 aaatcgccgt tgctgcctaa cttggtggcg ccggcgctgt agtaaccgag acccggcgca
     3481 tacagcgcca gccgcatcca ggcatcgaag ccgatgacgc caccatgctg ctgcatgaaa
     3541 gcgcgaatgt gctcggtgac gcgttgcgaa tgcgcgagtt catcggcatt cagcagcggc
     3601 agggttgtga tggacgcgga agaagtcata cagtgaggag gtgaaaagca gtgacggcag
     3661 tctaaccggc aagacggtgt tgatcacggg cggcgcccga cgtgtcggcg cggccattgc
     3721 gcgcatgctg cacggcgcag gcgcgaacct cgtcatccac taccgcaagt cggccaccga
     3781 ggccgcgtcg ctggccgatg atctcaatgc ggctcgcgcg aattccaccg tgattttcca
     3841 ggcggacctg ctcgacctcg gcaaattgcc ggcgctcgtg gagttcgccg tgcgccgctt
     3901 cggcgcgctc gacgtgctcg tgaacaatgc ctccacgttc taccccacga agatcggcga
     3961 gatcacgccg caggcctggg acgatctgat gggcagcaat ctcaaggcgc ctttgtttct
     4021 ctcgcaggcc gcggcccccg cgctgaagaa gtctggcggc ctcatcctca atattgtcga
     4081 tatccatgcg ctgcggccac tgcgcgatta cacggtctat tgttcggcca aggccggact
     4141 gcacatgctc acgcgttcgc tggcgaagga actcgggccg gagattcgcg tgaatggaat
     4201 ttccccgggt cccgtgatgt ggcccgagca cggcggcgac gaagcctcgc gcgccaagat
     4261 catccagcgc accatcctgc agaagatggg cacgcccgaa gacatcgcgc gcactgcgct
     4321 gttcttcgcc gcctcggcgc cgttcatcac cggacagatt ctggcggtcg atgggggccg
     4381 cagcgtcgcc tggtagtcag cccgccggca actccacggg cgtcatctga tgcccgtcgc
     4441 gatcgaactc ttcccacaac tggccgatgg tcttgtgcgc cgtgggatgc atcacctccg
     4501 ccgcgatctc cgccatcggc cccagcatgt atggccgctt cagaagatcc ggacgcggca
     4561 gcgtgtagtc ggcggtcttc tccacgcgat cgccgaacag cagcacgtcg agatccatgg
     4621 tgcgcgacgc ccacttcggc gcgaagcgcg gccggccgca tcgtgtttcg atggcgcgca
     4681 aatgcgcgat caccggatcc agcggttgcg aggtcgtgaa tcccagcacc aggttgatga
     4741 aatccggacc gtcgaaaccc accgccacgt tgcgatacgc acgcgagaac acggcgtcgt
     4801 gccacgcgtg tttgatctcg gcgcaggcgc ggacgagatt tttctcgggt tcgagattgc
     4861 tgccggccgc gacgaacacg cgcacgtggc cgggcttgag taggttcatg gaaactactt
     4921 cgggagatca gcgcgcgtgc gcagcacggc cacgcccacg tctttcgaac cgcggatggc
     4981 gccgggcttg ttgatggaca gacgcaccca ctcgatgccg aactcctgga ggacgagcaa
     5041 cgccgtgcga tgcgccagcg tctccaccag cttgaactcc gacccctcca cgaaagagat
     5101 gagccgcttg gcgaccctct tgtagtcgag cgtgtcggcg acgtcgtcgc tcgcggcggc
     5161 acgcgtgcag tccaccggca tctcgacgtc gagcacgatg gtctgcttga cgcgccgttc
     5221 ccattcgatg aagccgatga tgcattccac ggcgaggccg tgcagaaaga tgcgatcgcc
     5281 cttgtcggga gtggacgccg aactcggcag cgtgtagtcg gcggtcttct ccacgcgatc
     5341 gccgaacagc agcacgtcga g
//
LOCUS       DATDEU010000181         8470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1490424, whole genome shotgun sequence.
ACCESSION   DATDEU010000181 DATDEU010000000
VERSION     DATDEU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490424"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VIV63_04405"
     CDS             <1..930
                     /locus_tag="VIV63_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6123870.1"
                     /translation="SRVLQGATAGVGVVIGRAIIRDLYEGAAAQRLMNLTTMIFGLAP
                     AVAPIIGGWVHVAFGWRAVFAVMVVCGIVFSLGSWWKLPETHPPQARIPFNARNLVAT
                     SATVLRHPEFLMLSLAAAINFCSIACFIGSAPAIIEKHWGMSETSYAALFLPVITGIM
                     LGAVISRRIAGRMDLTRQVHMGLAITLAASVLRVALHLTLENVPIPLQQALLFGSAIG
                     AQFAFPVLTLRMLDLFPAARGTAASAQSFVALLVTAFTLGIVAPKVLPHLEWIAWTSL
                     LLTALASVCWHFARRWEKPPFPPDEPRKLAAGA"
     gene            985..1461
                     /locus_tag="VIV63_04410"
     CDS             985..1461
                     /locus_tag="VIV63_04410"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEY6123871.1"
                     /translation="MRTLELIALGALVSCTSATFAKSPASGGFDWLAGRWCVQSGESF
                     IEEHWLSPRGDLMLGVGRTVKGGKTMNFEFMRIEIRDGVASLIAQPQGGAPTAFRMTA
                     SGDHWARFENPQHDFPKRVEYRRTPDGLHAEIAGPGKNGKEAVIPFEYRGCGAEGG"
     gene            complement(1458..5150)
                     /locus_tag="VIV63_04415"
     CDS             complement(1458..5150)
                     /locus_tag="VIV63_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6123872.1"
                     /translation="MAVPESVEPSRFFAQFAAFIALVLGAFVLAGWSFDMAALTNLVP
                     GWPRISRLTAIGFFVAGVSLWLATLPMIRAAAAASLLLTAIGLLVLLRYALSWDAYID
                     QLLLSPMPALAHGGMPPRMAPATAVGFCCFGMSILCALRGPTALAHQTLAILVIMLGW
                     MGISRHIFGGEALVPFVNVAAHGSIVFLLLGAGALTLRTDAGVAKLLASSGVGGSMAR
                     RLLPAAVLVPLLAGALTLHVERNGTLGHEAAVSLFAISAVIAFTAFVLINAARGERAD
                     ASRRTAERALRISEERNQLIVETALDGVVTINHKGIITGWNSHAEKLFGWMRADALGR
                     ELGELLVPERLREQHRQGLQRYLESGVARVLNKRIEMSALHRDGTEFAVELAITPIGF
                     GDDLVFSAFIRDITSRNRAEAALRESEQRFRTTANAAPVLIWMSGKDKRCTWFNQRWL
                     DFVGRDIEQEIGDGWCDNLHPADFDRTLDTYHAAFDARRAYEMEYRLQRDDGAWRWLL
                     ERGCPHVGPSGEFVGFIGTCIDITEHRETVEQLRENRARFKTLAESLPQMIWTCLRDG
                     YCDYLSRQWLDYTGRSEAQQVGTGWLEQVHPDDRVKVDAEWARVVGSGDTYDISFRIR
                     RFDGVYRWFKTRAVPLRDPAGRILKWFGSNTDIEDFVLAERRLKVQLERMQLLDRTTH
                     AIGAHHELRKVFEVVLRSLEDNLDIDFATLCVYQSEPELLAVSCVGERSAELARGIGI
                     VEQARIEVNETGLKRCVQGELVYEPDIQNSRLPFGARLAGAGLRSLVASPLMIESEVF
                     GVMIVAKRGSEAFTSDDCEFLRQLSSHVALAAHQARLYEALQGAYQDLRQTQQTVMQQ
                     ERLRALGQIASGIAHDINNALSPASLYTQSMLAHEPNLTDRSREQLAVIQRAIDDVAR
                     TVQRMRAFYLPRGLELTLAPVDVNQILMQVIDLTRARWSNMPQERGVVVNVESDLAAD
                     LPMILGAEHELRDAFTNLLLNAVDAMPEGGTITLRTRFVERDDQVVIEVQDTGIGMSE
                     TTRSRCLEPFFTTKGERGTGLGLPMVFGMTQRHGGDFEIESELGRGTTMRLLFSRAPT
                     GMTVSERQLAPPSKPLRLLLIDDDPLLLRSLRDALELDEHEVVTADGGQAGIDAFALA
                     VKEGTVFDAVITDLGMPYVDGRKVAARVRQLSARVPIIMLTGWGHRLIVTDDKPEHVD
                     RVLSKPPKMMELRNTLAELAGSRDAN"
     gene            complement(5227..6891)
                     /gene="ubiB"
                     /locus_tag="VIV63_04420"
     CDS             complement(5227..6891)
                     /gene="ubiB"
                     /locus_tag="VIV63_04420"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY6123873.1"
                     /translation="MKLRVLSRLIEIQRVLVRHGLDEFVRATHLYRPLRFLFLLSPWT
                     WAVRRGDAPRAERLRLALEELGPIFVKFGQALSTRRDLLPIDIADELAKLQDRVPPFD
                     GKIARAVIEAAYGRAAEQVFSTFDENPLAAASIAQVHAAELRPEVALNGAKTREVVVK
                     VLRPGMHEIIARDLEVLRALAAFAEANWEGSQRLKPVEVVAEYEKTIIDELDLMREAG
                     NASQLRRNFENSPLLYVPAVHWDFCRPGVMVMERIRGVQISNMAKLREAGTDIRKLAE
                     NGVEIFFTQVFRHNFFHADMHPGNIFVLIDNPAQPKYAAIDFGIVGTLDPRDQYYLAE
                     NFLAVFERDYRKVATLHLESGWVPEGSRVDEMESAIRTVCEPIFNKPLKEISFGTVLL
                     RLFEISRRFNVSIQPQLILLQKTLLNIEGLGRDLYPDLDIFKTAGPIMRDWVREKMSG
                     RAILENARSQLPEILTSLQTLAPLVRSAVQRAQDGRLRLAVEAPEINALRTEIRRTNR
                     RRDKVTIAAVLLLGGIVWIALGREPDWAGWILSGAGAAWLLGLLRR"
     gene            complement(6888..7511)
                     /locus_tag="VIV63_04425"
     CDS             complement(6888..7511)
                     /locus_tag="VIV63_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY6123874.1"
                     /translation="MLTQAIENLLNRNLPRSPRALELCAALAGKRVRVEARALSWVLI
                     IESLTTSVRLTKGEAGGPPADAEISGTLLNLAALAGSHPEEVIQRGDVEIRGDAEVAQ
                     QFRELAMLLKPDVEEELSKIIGDTPAHQALRLVRMVTGFGRRAAETGVRNVAEYLAHE
                     RRDLVPRAEAEDFYRGVERLREDLDRLEARTRLLGETETEDEDEISR"
     gene            complement(7511..8269)
                     /gene="ubiE"
                     /locus_tag="VIV63_04430"
     CDS             complement(7511..8269)
                     /gene="ubiE"
                     /locus_tag="VIV63_04430"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY6123875.1"
                     /translation="MTDHKKNADTDFGYQRVPWNEKQRRVRGVFDSVAGNYDLMNDLM
                     SGGAHRLWKEFTLSLTSLRPGQRALDVAGGTGDLTTGLAKQVGKTGVVVLSDINAAML
                     GEGRDRMLDAGVVGNVSCVLANAEKLPFADSSFDCVTIGFGLRNVTDKAAALADMRRV
                     LKPGGQLLVLEFSQPVVPLLKPLYDLYSFRVLPLIGKLVAKDEDSYRYLAESIRMHPD
                     QETLLGMLRDAGLEDCRYHNLSGGIVAVHRGYRY"
BASE COUNT         1421 a         2705 c         2857 g         1487 t
ORIGIN      
        1 tcccgcgtat tgcaaggcgc gaccgcgggc gtgggcgtgg tgatcggccg cgccatcatc
       61 cgcgacctct acgagggcgc cgccgcgcag cgcctcatga acctgaccac catgatcttc
      121 ggcctggcgc cggccgtcgc acccatcatc ggcggctggg tgcacgtggc cttcggctgg
      181 cgcgcggtgt tcgcggtcat ggtggtatgc ggcatcgtgt tctcgctggg ctcgtggtgg
      241 aaattgccgg agacgcatcc gccgcaggca cgcatcccgt tcaatgcacg caacctggtg
      301 gcgacgtcgg cgacggtgtt gcgccatccc gagttcctga tgttgtcgct ggccgcggcc
      361 atcaacttct gctccatcgc ctgtttcatc ggctccgcgc ccgcgatcat cgagaaacac
      421 tggggcatga gcgaaacgtc ttatgccgcg ctgttcctgc cggtcatcac cggcatcatg
      481 ctcggcgccg tgatttcgcg gcgcatcgcc ggacgcatgg acctcacgcg ccaggtgcac
      541 atggggctgg cgatcacgct cgccgcctcg gtgctccgcg tggctctgca cctgactctc
      601 gagaacgtgc cgatcccgct gcagcaggcg ttgctgttcg ggtcggccat tggcgcgcag
      661 ttcgcatttc ccgtgctgac gctgcgcatg ctcgacctgt ttccggcggc gcgcggcacc
      721 gccgcctcgg cgcagtcctt cgtggcgctg ctggtcacgg cgttcacgct cgggatcgtg
      781 gcgccgaagg tgttgccaca cctggagtgg atcgcgtgga cgtcgctact gctcacggcg
      841 cttgcgtcgg tgtgctggca cttcgcgcgc cgctgggaaa agccgccatt cccaccggac
      901 gaaccgcgaa aacttgccgc tggcgcctga tgaacctcgg cgggttcgct ggattcccgc
      961 gcggcggggg agcacagtac gcgcgtgcgt acactcgaac tgattgccct tggcgcgctg
     1021 gtctcatgca cgagcgccac tttcgccaag tcgccggcat caggcggatt cgactggctc
     1081 gcgggccgct ggtgcgtgca aagcggtgaa tcgttcatcg aggaacactg gctgtcacct
     1141 cggggcgacc tgatgctggg tgtcggccgg accgtcaaag gcggcaagac gatgaacttc
     1201 gagttcatgc gcatagaaat ccgcgacggt gtcgcaagtt tgattgcgca gccgcagggt
     1261 ggggcgccaa ccgcgtttcg catgaccgca tcgggcgacc attgggcgcg cttcgaaaac
     1321 ccgcagcacg atttcccgaa gcgcgtggaa taccggcgaa cgccggacgg gctgcatgcg
     1381 gaaatcgccg gccccggcaa gaacggcaaa gaagccgtga tcccgttcga gtatcggggc
     1441 tgtggcgccg aaggcggcta gttagcgtcg cgcgagccgg cgagctccgc cagcgtattg
     1501 cgcagttcca tcatcttcgg cggcttgctc agcacccggt cgacatgctc gggtttgtcg
     1561 tcggtgacga tcagccggtg tccccagccc gtgagcatga tgatgggtac tcgcgccgag
     1621 agttgccgca cacgtgcggc caccttgcgt ccatcgacat agggcatgcc gaggtcggtg
     1681 atcacggcgt cgaaaacggt cccttccttc acggcgagcg cgaacgcgtc gatgccggcc
     1741 tggccaccgt cggcagtgac cacttcgtgt tcgtcgagct cgagggcatc gcgcagcgac
     1801 cgcagcagca gcggatcgtc gtcgatcagc agcagccgca gcggcttcga cggcggcgcg
     1861 agttgccgct cgctgacggt catgccggtg ggcgcgcgcg agaagagcag gcgcatggtc
     1921 gtgccgcggc cgagctcgct ttcgatctcg aagtcgccgc cgtgccgttg cgtcatgccg
     1981 aacaccatgg gcaggcctag accggtgccg cgttcgccct tggtggtgaa gaacggctcg
     2041 aggcagcggc tgcgcgtggt ctcgctcatg ccgatgccgg tgtcctgcac ctcgatcacg
     2101 acctggtcgt cgcgctccac gaagcgggtg cgcaacgtga tcgtgccgcc ctcgggcatt
     2161 gcgtcgaccg cgttcaacag caggttggtg aaggcgtcgc gcagctcgtg ttcggcgccc
     2221 aggatcatcg gcagatcggc agccaggtcg ctctcgacgt tcacgacgac accgcgttcc
     2281 tgcggcatgt tgctccagcg cgcgcgtgtg aggtcgatca cctgcatcag gatctgattg
     2341 acgtccaccg gcgccagcgt gagctccagc ccccgcggta gatagaaggc gcgcatgcgc
     2401 tgcacggtac gcgcgacgtc gtcgatggcg cgctggatga cggcaagttg ctcgcgcgac
     2461 cgatcggtca gattcggttc gtgcgccagc atggattgcg tgtagagaga ggctggtgac
     2521 agcgcattgt tgatgtcgtg cgcgatgcca ctggcgatct ggccgagtgc gcgcaggcgc
     2581 tcctgctgca tgacggtctg ctgggtctgg cgaagatcct ggtaggcgcc ctgcagcgcc
     2641 tcatacagcc gcgcctgatg ggcagccagc gccacgtggc tcgagagctg gcgcaggaac
     2701 tcgcagtcgt cactggtgaa tgcctccgag ccgcgcttcg ctacgatcat cacgccgaac
     2761 acttcgctct cgatcatgag cggcgacgcc accagcgaac ggaggccggc gccggcgagg
     2821 cgcgcaccga atggaagccg ggaattctgg atgtcgggtt cgtagaccag ttcgccctga
     2881 acgcagcgct tcaggccggt ttcgttgacc tcgatgcgcg cctgttcgac gatgccgatc
     2941 ccccgcgcca gctctgcgct gcgctcgccc acgcagctga ccgcgagcag ttcgggttcg
     3001 gactggtaga cgcacaaggt ggcgaaatcg atgtcgagat tgtcttcgag gctgcgcagc
     3061 acgacttcga acaccttgcg caactcgtgg tgtgcgccga ttgcgtgcgt ggtgcggtcg
     3121 agaagctgca tgcgctcgag ctgcaccttg agcctgcgct ccgcgagcac gaaatcttcg
     3181 atgtcggtgt tagagccgaa ccacttgagg atgcggccgg ccgggtcgcg cagcgggacc
     3241 gcgcgcgtct tgaaccagcg gtagacgccg tcgaagcggc ggatgcggaa gctgatgtcg
     3301 taggtatcgc cgctgccgac gacgcgggcc cactctgcgt ccaccttcac gcggtcgtcc
     3361 ggatggacct gctcgagcca acccgtgcct acctgctgtg cctcgctgcg gccggtgtag
     3421 tcgagccact gccggctcag gtagtcgcag tagccgtcgc gaaggcaggt ccagatcatc
     3481 tgcggcagtg attcggccag tgtcttgaag cgcgcgcgat tctcgcgaag ctgttcgacc
     3541 gtctcacgat gttcggtgat gtcgatgcag gtgccgatga atcccacgaa ttcaccgctg
     3601 ggtccgacat gcggacatcc gcgctcgagc agccagcgcc acgcgccatc gtcgcgctgc
     3661 agccggtatt ccatttcata ggcgcggcgc gcatcgaacg cggcgtggta agtgtcgagc
     3721 gtgcgatcga aatcggccgg gtgcaggttg tcgcaccagc cgtcgccgat ctcctgctcg
     3781 atgtcgcggc cgacgaagtc gagccagcgc tggttgaacc acgtgcagcg tttgtccttg
     3841 ccgctcatcc agatgagcac cggcgccgcg ttggcggtgg tgcggaaacg ctgttcgctt
     3901 tcgcgtagcg ccgcttcggc gcggttgcgg ctggtgatgt cgcgaatgaa ggcgctgaac
     3961 acgaggtcgt cgccgaatcc gatcggtgtg atcgcgagct ccaccgcgaa ctccgtgccg
     4021 tcacgatgca gcgccgacat ctcgatgcgc ttgttcagca cacgcgccac gccggattcg
     4081 aggtaacgct gcaatccctg ccgatgctgt tcgcgcagac gctcgggcac gaggagttcg
     4141 ccgagttcgc ggcccagcgc gtcggcccgc atccagccga aaagcttctc ggcatgcgaa
     4201 ttccagccgg tgatgatgcc cttgtggttg atcgtgacga cgccatcgag cgccgtttcg
     4261 acgatgagct ggttgcgttc ctccgagatg cgcagcgcgc gctccgcggt gcggcgcgag
     4321 gcatcggcgc gttcgccgcg cgcggcgttg atgagcacga atgcagtgaa ggcgatcacg
     4381 gctgaaatgg cgaacagcga caccgcggct tcgtgaccga gcgtgccgtt ccgttccacg
     4441 tgcagcgtca gcgcgcccgc gagaagcggc acgaggacgg ctgcggggag caggcggcgc
     4501 gccatgctgc cgccgacgcc gctgctggcc agcaacttcg cgactcccgc gtcggtgcgc
     4561 aacgtgagcg cgccggcgcc gagcaacagg aagacgatcg atccgtgtgc ggcgacgttc
     4621 acgaacggca cgagcgcttc gccgccgaag atgtgccgcg aaatgcccat ccagccgagc
     4681 atgatgacga ggatcgccag cgtctggtgc gcgagcgccg tcggcccgcg caacgcgcag
     4741 aggatcgaca ttccaaagca gcagaagccg accgcggtcg ccggcgccat gcgcggcggc
     4801 atgcccccgt gagcgagcgc cggcatcggg ctcaggagca gctggtcgat gtaggcgtcc
     4861 caggacagtg cgtaacgcag cagcaccagc aagccgatcg ccgtcagcaa cagcgatgct
     4921 gcggcggcgg cgcgaatcat gggcagggtg gccagccaca gggagacgcc ggcgacgaag
     4981 aagccaatgg ccgtcaggcg cgagatgcgc ggccagcccg gcaccaggtt ggtgagcgcc
     5041 gccatgtcga acgaccagcc cgcgaggacg aacgcgccca ggaccagcgc gatgaaagcg
     5101 gcgaactggg cgaaaaagcg actcggttca acgctctcgg ggacggccat gatgagtcaa
     5161 actactccga tgcccgcagt gccgcaatta gttagaacaa gtctcgtaac taggtactta
     5221 ccgaagtcag cggcgcagca agcctaacaa ccatgcggcg ccggcgccgc tcagtatcca
     5281 ccctgcccag tcgggttcgc gaccgagcgc gatccacacg atgccgccga gcagcagcac
     5341 cgcggcgatg gtcaccttgt cgcgccgccg attggtgcgg cggatctcgg tgcgcagtgc
     5401 attgatttcg ggtgcttcga ccgcgagccg cagccggcca tcctgcgcgc gctgcaccgc
     5461 ggagcggacg aggggcgcca gcgtctgcag gctggtcagg atttccggga gctgcgagcg
     5521 cgcgttctcg aggatggcgc ggccactcat cttctcgcgc acccagtcgc gcatgatcgg
     5581 gcccgcggtc ttgaagatgt cgaggtccgg atacaggtcg cggcccagcc cttcgatgtt
     5641 cagcagcgtc ttctgcaaca ggatgagctg cggctggatg gacacgttga agcggcgcga
     5701 gatctcgaac agtcgcaaca gcacggtgcc gaaggagatc tccttcagcg gcttgttgaa
     5761 gatcggctcg cacaccgtgc ggatcgccga ttccatttca tcgacgcgcg aaccctcggg
     5821 cacccagccc gactcgaggt gcaacgtggc gaccttgcgg tagtcacgct cgaacaccgc
     5881 gaggaaattc tcggcgaggt agtactggtc gcgcggatcg agtgtgccga cgatgccgaa
     5941 gtcgatcgcg gcgtatttgg gctgcgccgg attgtcgatg agcacgaaga tgttgccggg
     6001 atgcatgtcg gcatgaaaga aattgtggcg gaacacctgc gtgaagaaga tctccacgcc
     6061 gttttcggcc agcttgcgga tgtcggtgcc ggcctcgcgc aacttcgcca tgttggagat
     6121 ctgcacgccg cgaatgcgtt ccatgaccat gacgcccggg cggcagaaat cccagtgcac
     6181 ggccgggaca tatagaagag gagagttctc gaagttccgg cgcagctgcg aggcgttgcc
     6241 ggcttcgcgc atcaggtcga gctcgtcgat gatcgtcttt tcgtattcgg cgaccacttc
     6301 cacgggcttg agtcgctggc tgccttccca gttcgcttcc gcgaatgcgg ccagcgcgcg
     6361 caacacttcg agatcgcgcg cgatgatctc gtgcatgccg ggccgaagga ctttgaccac
     6421 cacttcacgc gtcttcgcgc cgttcaacgc cacctcgggg cgcagttcgg ccgcatgcac
     6481 ctgcgcgatg gaagccgccg ccagcgggtt ttcatcgaac gtcgaaaaca cctgttcggc
     6541 ggcgcggccg tatgcggctt cgatgaccgc acgcgcaatc ttgccgtcga agggcggtac
     6601 gcgatcctgc agtttcgcga gctcgtcggc gatgtcgatc ggcagcagat cgcgccgggt
     6661 ggagagcgcc tggccaaatt tcacgaagat gggtcccagc tcctcgagcg cgaggcgcag
     6721 gcgttcggcg cgcggcgcat cgccacggcg aaccgcccag gtccacggcg acagcaggaa
     6781 caggaatcgc aacgggcggt agaggtgagt ggcgcggacg aattcgtcca ggccgtgccg
     6841 taccagtacg cgctgaatct cgatgagccg ggacaggacg cgcagtttca tcgtgatatt
     6901 tcgtcctcgt cctcggtttc cgtctcaccg agcaggcgag tgcgggcttc gaggcgatcc
     6961 aggtcttcgc gcaggcgttc gacgccccga tagaaatctt ccgcttcggc gcgcggcacg
     7021 aggtcccgcc gttcgtgggc gaggtattcg gcgacgttgc gcacaccggt ctcggcggca
     7081 cgccggccga atccagtgac catgcgcacg agtctcaacg cctggtgcgc cggcgtgtca
     7141 ccaatgatct tcgagagttc ctcttcgacg tcgggcttga gcagcatcgc gagctcgcgg
     7201 aactgctgcg ccacttcggc gtcgccgcgg atttcgacgt caccgcgctg gatgacttcc
     7261 tcgggatgcg aaccggccag cgccgcgagg ttcagcaggg tgccggagat ctccgcatcc
     7321 gcgggcggtc cgcccgcttc gcccttggtg agcctgaccg aagtggtcag cgactcgatg
     7381 atcagcaccc agctcaacgc gcgtgcctcg acgcgcacgc gctttccggc gagcgcggcg
     7441 cacagctcga gcgcgcgcgg actgcgcggc aggttgcggt tcagcagatt ctcgatcgcc
     7501 tgggtcagca tcagtagcga tagccgcggt ggaccgcgac gatgccaccc gagaggttgt
     7561 ggtagcggca gtcctcgagt ccggcgtcgc gcagcatgcc taacaaggtt tcctggtccg
     7621 ggtgcatgcg gatggattcg gcgagatagc gatagctgtc ttcgtccttc gccaccagct
     7681 tgccgatgag cggcagcacg cggaacgaat agaggtcgta gagcggcttg agcagcggca
     7741 cgaccggctg ggaaaactcg agcaccagca actggccacc cggcttgagc acgcggcgca
     7801 tgtccgcgag cgccgcagcc ttgtcggtga cgttgcgcag gccgaagccg atggtcacgc
     7861 agtcgaacga ggagtcggcg aaaggcagct tctcggcatt ggcgagcaca caggacacgt
     7921 taccgactac gcccgcatcg agcatccggt cgcggccttc gccgagcatg gcggcgttga
     7981 tgtccgagag gaccacgacg cctgtcttgc cgacctgttt cgcgagcccg gtagtgagat
     8041 cgccggtgcc gcccgcgaca tcgagcgcac gctggccggg acgcaggctg gtcagggaga
     8101 gcgtgaactc tttccagagc cggtgcgcac cgcccgacat caggtcgttc atgaggtcgt
     8161 agttgccggc gacggaatcg aaaacacctc gcacacggcg ctgcttctcg ttccacggaa
     8221 cgcgttggta gccgaaatcg gtgtcggcgt tcttcttatg gtcagtcaca gggcggccag
     8281 tatagacgga gtggcaaatg tggctcgaaa gcactggctt ttcgggtctg cattcgctac
     8341 aaagcccaac tattcgagcg ggggtcttgg gaagggcggg ttacctaacg gcaacccgcc
     8401 ctttgtttat gtagcgggtc gcagacccgc gttgtattcg cgccgtgagg cgctggcaag
     8461 gctgcggccg
//
LOCUS       DATDEU010000182         9061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1344791, whole genome shotgun sequence.
ACCESSION   DATDEU010000182 DATDEU010000000
VERSION     DATDEU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344791"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..2263)
                     /locus_tag="VIV63_04435"
     CDS             complement(17..2263)
                     /locus_tag="VIV63_04435"
                     /inference="COORDINATES: protein motif:HMM:NF023683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123876.1"
                     /translation="MKRLFAATALAAIVTAGCSSSVDNPPDVTPTDPTTGNLAPPSTT
                     TLSANRVLFSPNQGILPYPHDAYFTPTTGVPTDGTLNLPSSAFFPATLRLPSGVTEPV
                     VNALDGFSTQAPIKLRFSTPVDLTTSAAGVKLIEVHIDPATKATIGFPTTSSPVRRVL
                     VQDVDYRVETAPNIDAGGTLVQIVPLRPLLPNNDAPGAARPRDIGYLVLVTNALRAAN
                     GAAIAADADYATIKTAALANTCATITDARLNALCNLARAHFGAAGAAGVSPATIVVSS
                     SFTTQSVDTVLRVITGSVAASPPPATAVLAAPVLPTGAISPTGPNLSDLHFATITLPY
                     YLGVPVAPPAGTGTEPITSWWRATGAPGAATGLQDPKNERNLTRFNPFPQVTTQLTVP
                     MLVGVPRTTKPAAGWPILIYQHGITEDRGTLALIADTAAAAGFVIVGIDLPLHGIMPT
                     DPLYALSPTNPMGPAYAGIPTAYRVGEPTFNVDYVNNTTSAPGPDGTPDSSGQHFINL
                     ASVLTARDNLRQGAANLIALTRAAPFLDVDGDAAPDIDPARIHFFGWSLGGIIGSAYM
                     GTPLAIANVKSVSLFAAGCGIMETLRQSPGYSPVLNNGLAAAGFPPGTSIYWEFVHAA
                     QATVESGDGCNYAANWIAKPTLMQMIQGTAGNATRPTDQAVPNSSTLRLANLLGLPTI
                     TATLTSGTGARALTRFTEGGHGTIALPSPAPQTLASYVEANTQLASFLGSGGAQITIT
                     NAAILAPQ"
     gene            2320..3045
                     /gene="rsmD"
                     /locus_tag="VIV63_04440"
     CDS             2320..3045
                     /gene="rsmD"
                     /locus_tag="VIV63_04440"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY6123877.1"
                     /translation="MQNDAPNLSYWGCSVRLGPKRWQATRRGHWHLAKAKNNGKSKDA
                     SRAHDAAGGARTSGGGSRVLRIIAGRHRGRRLRFPAGVEIRPTPDRVRETLFNWLQPR
                     MDQSRVLDLFAGSGALGLEALSRGAAQATFVEKDRRAAAAIEEVTREWQEANARVVCD
                     SAIRWLEAQTREPPFDIVFLDPPYDSDLLATAAAALVRRLAPDARVYLERRAREPLPP
                     LPESWKELRAGRAGEVGYHLFAV"
     gene            3059..3541
                     /gene="coaD"
                     /locus_tag="VIV63_04445"
     CDS             3059..3541
                     /gene="coaD"
                     /locus_tag="VIV63_04445"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY6123878.1"
                     /translation="MKRSAVYPGTFDPITNGHQDLVRRAAGVFERVIVAIASNPNKAP
                     MFPLEKRVELARAVLGDIRGVEVMGYKGLTVDFAREHDVQVVVRGLRAVSDFEFEFQL
                     ANMSRHLHRELETVFLTPQEQFTFISSTLVREIAVLGGDVKEFVHPLVAAELKKMKRV
                     "
     gene            3541..5226
                     /gene="ggt"
                     /locus_tag="VIV63_04450"
     CDS             3541..5226
                     /gene="ggt"
                     /locus_tag="VIV63_04450"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6123879.1"
                     /translation="MRAFVCLVFALLALPLQAATKVTKIPAGGPGKAAIASAYPLASD
                     AGKEILAKGGNAFDAAVAVAAALAVVEPSSSGLGGGGFFLLRRAKDGLETMIDLREMA
                     PGAATRDMYLDKDGNVVAGLSRDTALAAGIPGEPAGIAHLAKKYGKLPLSVSLAPAIK
                     LARDGFPLYTRLRGGMEFKKAAFLKTPDATRVYLVNGEVPELGAVIRQPDLAASLEIL
                     AAQGADGFYKGAFAKKLVAGVQSLGGNWTEADLANYKIVERPPVVGHYRGARIVSGSP
                     PTSGGIALIDALNILDGFDLAKVDKVTRTHLIVEAMRRVHRDRAVYLGDPDFVDVPVE
                     RLINEYYAAGQRASIRMDKATPSASLPGVDAPSPGPSTTHFSVIDAKGNMVAATITLN
                     FFFGSGLMVPGTGILLNNQMDDFSVKPGVPNGFQLIGGDANAIAPGKRPLSSSTPTFV
                     EGPNGLMILGSPGGSYIPGMVILGTLNFMDGKSAQEIVAAPRFHHQFSPDVLQFETDA
                     LNADERKALEDRGHKLREGTRKWGNLQVVIWDYKTGKVEAASDPRGAGVGLVY"
     gene            complement(5223..6533)
                     /locus_tag="VIV63_04455"
     CDS             complement(5223..6533)
                     /locus_tag="VIV63_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6123880.1"
                     /translation="MSFRKLPFVALLAIACATTPPAWGADCRIGLYQLANQDFVDIAP
                     AGDSKLRWRRPDGTSGALSENTDGSWSSTLGWTGKPDGRRISFTSCDAGGIEFDGIAG
                     RRAALETRELKFESAGATLAGRLVMPAGKSKVPLVVLVHGSEQMSALEFYALQRLFPS
                     AGIGVFVYDKRGTGASTGTFTHDYHALAADAAEAVRTARKLAGARAKRVGFQGSSQGG
                     WVAPLAATLTPVDFIVVGYGLAVSPFDEDNEAVALDMTRHGFGEAETARALEVAHAAQ
                     AIVLNGFQSGYEELDAVRARYAHEPWLKFVRGNVTQVILSQPRDVLREHGPVLFAGIL
                     PHYDPMPVLRRLDTPQLWILAADDIDAPVAETVRRLRSLSKEGRPITTVIYPRTEHGL
                     YEFEVDAAGERLSTRQPGSYLPLMVDFIRRGNIRARYGDSTIYR"
     gene            complement(6578..7372)
                     /locus_tag="VIV63_04460"
     CDS             complement(6578..7372)
                     /locus_tag="VIV63_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123881.1"
                     /translation="MNTPASMCSPAELTAFKRRLAKIDPILANVIRAAGKFTHATSAQ
                     HSPFHSLARAIAHQQLNGIAAESIFGRFVGLYAVEGKAAALLEAELVLTTPDDKMRAV
                     GLSFAKIASIKDLAQKTIDGVVPPSEILHTLADEEIIERLTQVRGIGRWTVEMMLMSR
                     LGRPDVLPIDDFGVRNGFRLAYGLRGMPTPRALAEFGVCWAPYRSVAAWYLWRAVDLH
                     RLGKLPEPAMPTRIKIVKKKKAVKKVATKKAKRPKKKRAGKRATRR"
     gene            complement(7395..8210)
                     /gene="mutM"
                     /locus_tag="VIV63_04465"
     CDS             complement(7395..8210)
                     /gene="mutM"
                     /locus_tag="VIV63_04465"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY6123882.1"
                     /translation="MPELPEVETTRRGIEPHVTGRRIARLLVHDFRLRWPVDPQIVAA
                     VAGSAIRRAGRRAKYLLLETDAGTLILHLGMSGSLRVLPVLTPRITHDHVDIELDSGQ
                     TLRFNDPRRFGSLLFTTGDPSSHPLLKSLAPEPLEDDFNGDYLWKITRRRAVAIKQLI
                     MNSRLVVGVGNIYASEALFRARVRPRRQARKLSRAECNKLARAIKQTLALAVKVGGTT
                     LRDYVGADGNPGYFRQKLYVYERAGMACRVCDKPVKQFTQGGRSTYWCPNCQK"
     gene            complement(8502..8657)
                     /gene="rpmG"
                     /locus_tag="VIV63_04470"
     CDS             complement(8502..8657)
                     /gene="rpmG"
                     /locus_tag="VIV63_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY6123883.1"
                     /translation="MREKVKLLSSAGTGHFYVATKNKRLHPDKMNVKKYDPVVRKHVD
                     YKEAKIK"
     gene            complement(8661..8897)
                     /gene="rpmB"
                     /locus_tag="VIV63_04475"
     CDS             complement(8661..8897)
                     /gene="rpmB"
                     /locus_tag="VIV63_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY6123884.1"
                     /translation="MSRVCEVTGKSVAVGNRVSHANNKKRRRFLPNLHTQRFWLETER
                     RWVSLRISTSGLRTIEKNGIESVIAGMRAKGKKV"
BASE COUNT         1545 a         2819 c         3049 g         1648 t
ORIGIN      
        1 ggaaactgcc gcgagactac tgcggcgcga gtatcgccgc gttggtgatt gtaatctgcg
       61 cgccgccgga gcctaagaac gatgcgagtt gcgtgttggc ttcgacgtag gacgcaagcg
      121 tctggggtgc cgggctcgga agcgcgatcg tgccgtgtcc gccctccgtg aagcgcgtga
      181 gggcgcgcgc accggtcccg ctcgtgagcg tcgcggtgat ggtcggcaac cccagcagat
      241 tcgcaaggcg aagggtcgag gagttgggca cggcctggtc tgtcggacgc gtggcatttc
      301 ccgccgtgcc ctggatcatc tgcatgagcg tcggcttggc gatccagttc gcggcgtagt
      361 tgcagccgtc accggattcc accgtggcct gcgctgcgtg cacgaactcc cagtagatgg
      421 aggtgcctgg cgggaagccc gcggccgcga gaccgttgtt gagcaccggt gaatatcccg
      481 gcgactggcg cagcgtctcc atgattccgc agcccgccgc gaacagcgac accgacttca
      541 cgttcgcgat ggccagcggc gtgcccatgt aggcgctgcc gatgatgccg ccgagcgacc
      601 agccgaagaa atggatgcgt gcggggtcga tatccggagc ggcatcgcca tcgacatcga
      661 ggaacggcgc cgcgcgtgtg agcgcgatga gattcgccgc accctggcgc aggttgtcgc
      721 gcgccgtcag cacactcgcg aggttgatga agtgctgacc ggacgaatcg ggagtgccat
      781 cgggtccggg agccgacgtg gtgttgttca cgtagtccac gttgaacgtg ggttcgccga
      841 cacgataggc cgtcgggatg ccggcgtacg ccgggcccat gggattcgtg ggcgagagcg
      901 cgtacagcgg atcggtcggc atgatgccgt gcaggggcag gtcgatgccg acgatgacga
      961 agcccgcagc cgccgcggtg tccgcaatca gcgcgagggt gccgcgatct tcagtgatgc
     1021 cgtgctggta gatgagtatc ggccagcccg ccgcgggctt ggtggttctc ggcacaccca
     1081 cgagcatggg caccgtgagt tgcgtggtga cctgcgggaa tggattgaag cgcgtgagat
     1141 tgcgctcgtt cttggggtcc tggagtcccg tggccgcccc gggcgcaccc gttgcacgcc
     1201 accagctcgt gatcggctcg gtgccggtgc ccgcgggcgg cgctaccggc acgcccaggt
     1261 agtaaggcag cgtgatggtc gcgaagtgaa gatccgagag attcgggccg gtcggcgaga
     1321 tcgcgcctgt cggaaggacc ggcgccgcca gcacggcggt ggcgggaggc gggctcgcgg
     1381 caacggaccc ggtgatcacg cgcagcacgg tgtccaccga ttgcgtggtg aagctcgagg
     1441 aaacgacgat agtcgccggg ctcacacccg cggcacccgc ggcgccgaag tgcgcgcgcg
     1501 ccaggttgca gagcgcgttc agtcgcgcat cggtgatggt ggcgcaagtg ttcgccagcg
     1561 cagccgtctt gatggtggcg tagtcggcgt ccgcggcgat ggcggcgccg ttggcggcgc
     1621 gcagcgcgtt cgtgacgagc acgaggtagc cgatatcgcg cgggcgggcc gcgcccggcg
     1681 cgtcgttgtt cgggagcagc ggccgcagcg gcacgatctg cacgagcgtg ccaccggcat
     1741 cgatgttagg cgcggtctcg acgcggtaat cgacgtcctg caccagcacc cggcgcacgg
     1801 ggctcgaggt ggtcgggaag ccgatggtcg ccttggtcgc cggatcgatg tggacttcga
     1861 tgagcttgac gcccgcggcc gaagtcgtga gatcgaccgg cgtggagaaa cgcagcttga
     1921 tcggggcctg cgtggagaat ccatccagcg cattgaccac gggttcggtg acgccactcg
     1981 gcaggcgcag cgtggccggg aagaacgcgc tggacggcag gttcaacgtt ccgtcggtgg
     2041 gcacaccggt ggtcggtgtg aagtaggcgt cgtgcggata cggcaggatg ccctggttcg
     2101 gcgagaacaa cacacggttg gccgacagcg tggtggtgct ggggggtgcg aggttgcccg
     2161 tggtcggatc cgtgggggtg acgtcaggtg gattgtcgac ggaactcgaa caaccggccg
     2221 tgacgatggc agccagtgca gtggctgcaa aaagcctctt catgagttca ctcctcggaa
     2281 ttctgtttcg gagaatcagt ccggtcgcat tccctcgccg tgcagaacga tgcgccgaac
     2341 ttgtcgtatt ggggctgtag cgtacgcttg ggccccaaaa ggtggcaagc cacgcgacgc
     2401 gggcactggc atttggcaaa agcgaaaaac aacgggaaaa gcaaagatgc cagtcgcgcc
     2461 catgatgcag cgggtggtgc acgaacatcc ggcggcgggt cgcgcgtcct gcgcatcatc
     2521 gcgggccggc atcgcggtcg ccgcctgcgt tttcctgccg gcgtggagat tcgtccgaca
     2581 ccggacaggg tgcgggaaac cctgttcaac tggctgcagc cgcgcatgga ccagtcacgc
     2641 gtgctcgact tgtttgccgg cagcggagca ctcggtctcg aagcactgtc acgcggcgcc
     2701 gcgcaggcca cgttcgtcga aaaagatcgg cgcgccgcgg ctgccataga agaagtgacc
     2761 cgcgaatggc aggaggcgaa cgcgcgcgtc gtctgtgata gcgcgatccg gtggctcgag
     2821 gcacagaccc gcgaaccacc attcgacatc gtgttcctcg atccacccta cgattcggat
     2881 ctgctggcca cggcggccgc ggcattggtc cgccggctcg cgcccgacgc gcgtgtctat
     2941 ctcgaaaggc gtgcccggga gccgttgccg ccactgcccg agagctggaa agagcttcgc
     3001 gcgggtaggg ccggcgaggt cgggtatcat ctatttgctg tttgaaaccc aaggccacat
     3061 gaaacgcagc gccgtttatc cggggacctt cgatcccatc accaacggac accaggatct
     3121 ggtgcgccgc gccgcgggtg tgttcgaacg cgtcatcgtc gcgatcgcct cgaaccctaa
     3181 caaggcgccg atgttcccgc tggaaaagcg cgtggaactg gcgcgcgcgg tactcggcga
     3241 catccggggc gtcgaagtca tgggatacaa gggactcacc gtcgatttcg cgcgtgagca
     3301 cgatgtgcag gtcgtggtgc gcggacttcg cgcggtgtcg gacttcgaat tcgagttcca
     3361 gctcgccaac atgagccgcc acctgcaccg cgagctcgaa accgtgtttc tcactccgca
     3421 ggaacagttc accttcattt cctccacgct ggtgcgtgag atcgcggtcc tcggtggaga
     3481 cgtcaaggag ttcgtgcacc cgctggtcgc cgccgagctc aagaagatga agcgggtctg
     3541 atgcgcgcat tcgtttgcct cgtattcgcg cttctcgcac tgccgcttca ggcggcgaca
     3601 aaggtcacga agattcccgc gggcgggccg ggcaaggcgg ccatcgccag cgcctatcca
     3661 ctcgcgagtg acgcgggcaa ggaaattctc gccaagggcg gcaatgcatt cgatgccgcg
     3721 gtcgccgtcg ccgcggcgct tgccgtggtc gagccgtcga gttcgggcct gggcggcggc
     3781 gggttcttcc tgcttcgccg cgcgaaagac gggctcgaga ccatgatcga tctgcgcgaa
     3841 atggcgccgg gcgccgccac gcgcgacatg tatctcgaca aggacggcaa tgtcgtcgcc
     3901 gggctgtcac gcgatacggc gctcgcggcg ggaatacccg gcgagcccgc gggcatcgcc
     3961 catctcgcga agaagtacgg caagttgcct ctgtcggtca gcctcgcccc ggccatcaaa
     4021 ctggcgcgcg acggcttccc gttgtacacg cgcctgcgcg gcggcatgga attcaagaag
     4081 gccgccttcc tcaagactcc cgatgccacg cgcgtgtatc tcgtgaacgg tgaagtgccc
     4141 gaactcggtg ccgtcatccg gcagccggat ctggccgcca gcctcgagat actggccgcg
     4201 cagggtgccg acggtttcta caagggcgcg ttcgcgaaga agctggtggc cggcgtgcag
     4261 tcgctcggcg gtaactggac cgaagccgac ctggctaact acaagatcgt cgagcgtccg
     4321 cccgtggtcg ggcactaccg cggcgcacgc atcgtgtcgg gttcgccgcc gacgtcgggc
     4381 ggcatcgcgc tcatcgatgc gctgaacatc ctcgacggtt tcgatctcgc gaaggtcgac
     4441 aaggtcacgc gcacccatct catcgtcgaa gccatgcgcc gcgtgcatcg cgatcgggcc
     4501 gtgtacctcg gcgatcccga tttcgtcgac gttcccgtgg aacggctcat caacgagtac
     4561 tacgcggccg gccagcgtgc gtcgatccgc atggacaagg ccacgccgag cgccagcttg
     4621 ccgggcgtcg atgcgccgtc accgggcccg tcgaccacgc atttctcggt gatcgacgcc
     4681 aagggcaaca tggtggcggc gaccatcacg ctcaatttct tcttcggatc cggcctcatg
     4741 gttcccggca ccggcatcct gctgaacaat cagatggacg atttctccgt gaagccgggt
     4801 gtgcccaatg gcttccagct catcggcggc gatgcgaacg ccatcgcgcc cgggaaacgc
     4861 ccgctgtctt cttccacgcc cacgttcgtc gagggcccga acggcctcat gatcctgggc
     4921 tcgccgggcg gcagctacat cccgggcatg gtcattctcg gtacgctgaa tttcatggac
     4981 gggaagagcg cgcaggagat cgtcgccgcg ccgcgcttcc accatcagtt ctcaccggac
     5041 gtgctgcagt tcgaaaccga tgcgctcaac gcggatgaac gcaaggcgct ggaagaccgg
     5101 ggccacaagc tgcgcgaagg cacccgcaag tggggcaacc tgcaggtcgt catctgggac
     5161 tacaagaccg gcaaggtcga agccgcttcc gatcctcgtg gcgcgggcgt ggggctcgtc
     5221 tactagcggt agatagtgct gtcgccgtag cgcgcgcgga tgttgccgcg ccggatgaaa
     5281 tccaccatca atggcagata actcccgggc tggcgcgtag atagccgttc gccggccgca
     5341 tccacttcga attcgtacag gccgtgttcg gtgcgtgggt aaatcaccgt ggtgattggc
     5401 cggccctctt ttgatagcga gcgcaagcgg cgaaccgtct ccgccacggg cgcgtcgata
     5461 tcgtccgcgg cgagtatcca caactgcgga gtgtcgagcc ggcgcagcac gggcatgggg
     5521 tcgtagtggg gcaggatgcc ggcgaacagc acgggtccgt gttcgcgcag cacatcgcgc
     5581 ggctgcgaaa ggatgacctg ggtgacgttg ccacggacga acttcagcca cggctcgtgc
     5641 gcatagcgtg cccgcaccgc gtcgagttct tcgtaacccg actgaaatcc gttgagcacg
     5701 atcgcctgcg cggcatgcgc gacttccagc gccctggcgg tttcggcttc gccgaatccg
     5761 tgacgtgtca tgtcgagcgc gacggcctcg ttgtcttcgt cgaacggcga caccgccagt
     5821 ccgtaaccga ccacgatgaa gtcgacgggt gtcagcgtgg ctgcgagcgg cgcgacccaa
     5881 cctccctggc tgctgccctg aaacccgacg cgcttcgcac gtgcgccggc cagtttacgc
     5941 gccgtgcgca cggcttctgc cgcatcggcg gcgagcgcgt ggtagtcgtg ggtgaatgtg
     6001 ccggtggatg cgcccgtgcc acgcttgtcg tagacgaaca cgccgatacc cgcggacggg
     6061 aatagccgct gcaacgcata gaactcgagt gccgacatct gttcggaacc atgtaccagg
     6121 acgacgagcg gaactttcga tttgcccgcg ggcatcacga gacggccggc gagcgttgcg
     6181 ccggcgcttt cgaatttcag ttcccgcgtc tcgagcgcgg cccggcggcc ggcgatgcca
     6241 tcgaactcga taccgccggc gtcgcaggat gtgaatgaaa tccgtcggcc atccggtttt
     6301 ccggtccatc cgagggtgct gctccacgag ccatcggtat tttcactgag cgcgccgctc
     6361 gtgccgtcag gacggcgcca tcgcaacttc gaatcgccgg ccggcgcgat gtcgacgaaa
     6421 tcctggttcg cgagctgata cagcccgatg cggcagtcgg cgccccaggc aggtggcgtg
     6481 gttgcgcacg cgatcgcgag gagcgctacg aacggaagtt ttctgaagga cattactcac
     6541 cagcgggttg attgacgctg gctttagacg cggcagccta tcggcgggtt gcacgcttgc
     6601 cggcgcgttt tttcttcggg cgcttcgcct ttttcgtggc gactttcttc accgctttct
     6661 tcttcttcac gatcttgatg cgcgtgggca tcgcaggttc gggaagtttg cccagccggt
     6721 gcaggtccac ggcgcgccac agataccacg ccgccaccga gcgatacggc gcccagcaca
     6781 cgccgaactc ggcgagtgcg cgcggcgtcg gcatgccacg caatccgtag gcaaggcgaa
     6841 aaccattgcg cacgccgaag tcgtcgatgg gcagcacgtc gggacgaccg agccgcgaca
     6901 tgagcatcat ctcgactgtc cagcgcccga tgccacgcac ctgcgtgagc cgctcgatga
     6961 tttcctcgtc cgcgagcgtg tgcaggatct ccgacggggg cacgacaccg tcgatggttt
     7021 tctgcgcgag atccttgatg ctcgcgatct tcgcgaacga gagacccacc gcgcgcatct
     7081 tgtcgtcggg cgtcgtgagc acgagttccg cttcgagcaa ggccgccgcc tttccctcca
     7141 ccgcgtacag cccgacaaaa cggccgaaga tgctttccgc cgcgatgcca ttcaattgct
     7201 gatgcgcaat cgcgcgcgcc agcgaatgaa acggcgaatg ctgcgcgctg gtggcgtggg
     7261 tgaatttgcc ggcggcgcga atgacattcg caagaatcgg gtcgatcttc gcgaggcggc
     7321 gtttgaacgc cgtgagctcg gccgggctgc acatggatgc gggtgtgttc acgcgcggat
     7381 attagccgct cgctctactt ctggcagttc ggacaccagt aagtcgagcg gccaccctgc
     7441 gtgaattgtt tgacgggctt gtcgcaaacg cggcacgcca tccccgcgcg ttcgtaaacg
     7501 tagagcttct ggcgaaaata tcccgggttg ccatcggcgc cgacgtagtc gcgcaacgtg
     7561 gtgccaccga ctttcacggc caacgcgagc gtctgcttga tcgcgcgcgc gagcttgttg
     7621 cattcggcgc ggctcaattt gcgcgcctgg cggcgtggcc gcacacgcgc gcggaacaac
     7681 gcctcgctcg cgtagatgtt gcccacaccc acgaccagtc tcgaattcat gatgagctgc
     7741 ttgatcgcga ccgcgcgtcg ccgcgtgatc ttccatagat agtcgccgtt gaagtcatcc
     7801 tcgagcggtt cgggcgcgag actcttgagc aacggatggc tcgatggatc accggtggtg
     7861 aacagcaggc tgccgaagcg gcgcggatcg ttgaaacgca acgtctgccc cgagtcgagt
     7921 tcgatgtcga cgtgatcgtg cgtgatgcgc ggcgtcaaaa ccggcagcac gcgcaggctg
     7981 cctgacatcc cgagatgcag gatcaacgtg cctgcatcgg tttcgagcaa cagatatttc
     8041 gcgcggcggc ccgcgcgccg gatcgcgctg ccggcaacag ccgcaactat ttgcgggtcg
     8101 acgggccatc gcagccggaa gtcgtgcacc agcaagcgcg cgatgcgccg tccggtgaca
     8161 tgcggctcga tgccgcggcg cgtggtttcg acttcgggta gttcgggcac gtgtaggggc
     8221 gggtcagctc gacgcgaata tcgatccatt ggtcgctttg ggctgcaacc ctttgggctt
     8281 ttcgtgcgtc tatacgggta cgaacgattg tgggtggcgg cgggtccctc ctgcaagaga
     8341 ccccagtgat gacgctcacc agagctaatc gcgtacccgc cgccgcccca gaatcttcgt
     8401 tgtaattttt ggtgagtgtc gattaggcaa ataaaaaagg cccgctgttg ttgcagcggg
     8461 cctttttcgt atccggatcc gtcacgcctt gcggcgcgag atcacttgat cttcgcttcc
     8521 ttgtagtcga cgtgcttgcg cacgaccggg tcgtacttct tcacgttcat cttgtcgggg
     8581 tgcaggcgct tgttcttcgt ggccacgtag aaatggccgg tgccggcgga cgacagcagt
     8641 ttcacttttt cacgcatgac ttagaccttc ttgcctttgg cgcgcatgcc agcgatgacg
     8701 gactcgatgc cgttcttctc gatggtgcgc agaccgctgg tggaaatgcg caggctcacc
     8761 caccggcgct ccgtctccag ccagaagcgc tgggtgtgca gattcggcag gaagcggcgg
     8821 cgcttcttgt tattggcgtg ggagacgcgg ttgccgacag caacgctctt gcccgtgact
     8881 tcgcagacgc ggctcataaa aatcccagaa aatcagttgg ttactaacgg acgaccgggt
     8941 tacgggcgtc gaaagggccg cctttatacc agccggtcgg gttgtgggca actcaagcca
     9001 gctggcatcc gtcacatcgt ggcgatggtt cgcctgcgcc gagggcaagg ggagcgttca
     9061 t
//
LOCUS       DATDEU010000183         3786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1053329, whole genome shotgun sequence.
ACCESSION   DATDEU010000183 DATDEU010000000
VERSION     DATDEU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053329"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1212)
                     /locus_tag="VIV63_04480"
     CDS             complement(7..1212)
                     /locus_tag="VIV63_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6123885.1"
                     /translation="MEIRPILSALLRNRAGAILVSLQIALTLAIVVNAVYVTKQRVDK
                     IGRPSGIDDQNIFSFPATGFEKDFDFNAMVREDMALIRQMPGVIDAAPIQQVPLSGSG
                     SSSGYYSLPDKKGVESPANYFPTDEHGVNTLGLKLSGGATFDPSTVIFRKDYTQDPPP
                     LAIVSQGFASVLFPKENALGKTFYDDQSKPFKIVAVIEHMHGSWVGWDKVDRTVLFPM
                     VGPGPGARYIVRTQPGEIDRVMSAVETAVRKRDPNRVIGKLRKMSEHKKASYAGDSLM
                     AVTLSSVTGLVLIFSALGIFGLATFNVNTRTRQIGTRRAVGARRRDIVRYFMIENWMV
                     TTLGVVAGCGLALAAGFWLSTHFSLPRLDLYYLVAGVFGLWIVGQLAAWQPSLKAAKV
                     SPAMATRTV"
     gene            complement(1214..2560)
                     /locus_tag="VIV63_04485"
     CDS             complement(1214..2560)
                     /locus_tag="VIV63_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6123886.1"
                     /translation="MFGYYLRLAVQSFRRNPGLTALMVFAIALGISVCMITLTSFRAA
                     AHNPAGERAGILYAASIDSWDPDNAYDSDQKDPETGKSLAPTMLTYRDAQALYASSIP
                     DRKVIMFKAGGIFSRPDKGMEPEYIATRMTTGDFFPMFEVPFLYGGSWDAKADTGPEP
                     VVVLSKETNQKAFGGANSVGKTVLWHDREFRVVGVLDHFEPYPKYFDVSNGSFSDMEG
                     AYIPFAWGKVLELGSDGNTNCWKTEKIDSYQDFLNSECIWFHGWFEMRSADKARAFRE
                     FLDNYVTEQKKHGRFPRPLNNYLYDVSGWLKRNKVVEDDNKAMLVLAFAFLAVCLVNT
                     IGLMLAKFLNAAPVAGVRRALGASRRDIFWQHLTESGVVALAGGILGGLLGITGIWAL
                     RAWYGRFEDDTQRVLPFDEMNFLIVIAIAVFAGLIAGLYPAWRIGRAAPASYLKAQ"
     gene            complement(2564..3289)
                     /locus_tag="VIV63_04490"
     CDS             complement(2564..3289)
                     /locus_tag="VIV63_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6123887.1"
                     /translation="MTNVSKVFRTDLIETHALRNFSLQVKAGEFLAVTGPSGSGKTTF
                     LNIAGLLEPYETGSYKLDDVEVTALSDDARSHLRNQKIGFIFQSFNLMPDLNVYDNVD
                     VPLRYRRMKSAERDKRITNALEIVGLAARMKHVPAQLSGGQQQRVAIARAIAGDPKLV
                     LADEPTGNLDSLMARQVMDLLERINEMGTTIIMVTHDPELARRAHRNVQVVDGQISDF
                     KPYEPSAAPPHGATAHVTALAGA"
     gene            complement(3313..>3786)
                     /locus_tag="VIV63_04495"
     CDS             complement(3313..>3786)
                     /locus_tag="VIV63_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY6123888.1"
                     /translation="APLVTVVDLTALEIEFQVAESYANEIRSGMNAEITLDGRKLVGT
                     VAGISPDVRNAQVTGRVRFAEQPQGMRQNQRASVRIVLDERNDVLKVARSPFNDSDTR
                     FIYVVRDDDAVRTPVEFGAAAIGEIEIRNGLNVGDTVVLSDMRDYKDAPSVLIGN"
BASE COUNT          654 a         1132 c         1294 g          706 t
ORIGIN      
        1 agatgttcag acagttcggg tcgccatggc gggcgagacc ttggcggcct tcagggaggg
       61 ctgccaggcg gcgagctgac ccacgatcca cagaccgaac acgccggcga ccaggtagta
      121 caggtcgagt cgcggcagcg agaaatgggt ggatagccag aagccggcgg ccagtgcgag
      181 accgcagccc gcgaccacgc cgagcgtcgt gaccatccag ttctcgatca tgaagtaccg
      241 cacgatgtcg cgccggcgcg cgcccacggc gcggcgggtg ccgatctgcc gcgttcgcgt
      301 gttcacgttg aacgtggcga gtccgaagat gccgagcgcg ctgaagatca gcacgagtcc
      361 ggtgaccgaa gagagagtca ccgccatcag cgagtcgccg gcgtagctgg ccttcttgtg
      421 ttcggacatc ttgcgcagct tgccgatgac ccggttgggg tcgcgcttgc gcacggcggt
      481 ttccaccgcg gacatcacgc gatcgatctc gccgggctgc gtgcgcacga tgtaacgcgc
      541 gcccggaccg ggcccgacca tcgggaacag gacggtgcga tccaccttgt cccaacccac
      601 ccaggagccg tgcatgtgtt cgatgacggc gacgatcttg aacggcttgc tctgatcgtc
      661 gtagaaggtc ttgcccagtg cgttctcttt ggggaacagc acgctcgcga atccctggct
      721 gacgatcgcc agtggcggcg ggtcctgggt gtagtccttg cggaagatca cggtactggg
      781 gtcgaacgtc gcgccgcccg acagcttgag tcccagcgtg ttcacgccgt gctcgtcggt
      841 ggggaagtag ttggctggcg actccactcc cttcttgtcc ggcaacgagt agtacccgga
      901 ggaactgccg ctgccggaaa ggggcacctg ctggatgggt gcggcgtcga tcacgcccgg
      961 catctggcgg atcagcgcca tgtcctcacg caccatggca ttgaagtcga agtccttctc
     1021 gaaacccgtg gcggggaacg agaagatgtt ctggtcgtcg atgccgctcg gccggccgat
     1081 cttgtcgaca cgctgcttgg tgacgtagac ggcattgacg acgatggcga gcgtgagcgc
     1141 gatctgcagc gacaccagga tggcgccggc gcgattgcgc agcagcgcgg acaggatcgg
     1201 tcggatttcc atgttattgc gccttcagat aagaggcggg cgctgcacgc ccgatgcgcc
     1261 acgcgggata caggccggcg atcaggccgg cgaagaccgc gatagcgatg acgatgagga
     1321 aattcatctc gtcgaagggc aacacgcgct gcgtgtcgtc ctcgaagcgt ccgtaccagg
     1381 cgcgcagcgc ccagatcccg gtgatgccta acaatccgcc gaggatgccg ccggccagcg
     1441 ccacgacgcc ggattccgtc aggtgctgcc agaagatgtc gcgacggctc gcgccgagcg
     1501 cgcggcgcac gcccgcgacc ggtgcggcat tgaggaactt cgcgagcatg agaccgatgg
     1561 tgttcaccag gcacaccgcg aggaacgcga acgccagcac cagcatggcc ttgttgtcgt
     1621 cttcgacgac cttgttgcgc ttgagccagc cgctcacgtc gtacaggtag ttgttgagcg
     1681 gccgcgggaa gcggccgtgc ttcttctgtt cggtcacgta gttgtcgagg aactcgcgga
     1741 acgcgcgcgc cttgtcggcg ctgcgcatct cgaaccagcc gtggaaccag atgcattcgg
     1801 aattgaggaa gtcctggtag ctgtcgatct tctcggtctt ccagcaattg gtgttgccgt
     1861 cgctgccgag ctcgagaacc ttgccccagg cgaacgggat gtaggcgcct tccatgtcgc
     1921 tgaaagagcc gttgctcaca tcgaaatact tcggatatgg ctcgaagtgg tcgagtacgc
     1981 ccacgactcg gaattcgcgg tcgtgccaga gcacggtctt gccgacgctg ttggcgccgc
     2041 cgaaggcctt ctgattggtc tccttgctga gaacgaccac cggctcgggc ccggtatcgg
     2101 ccttggcgtc ccacgaaccg ccgtagagga acggcacctc gaacatcggg aagaaatcgc
     2161 ccgtggtcat gcgcgtcgcg atgtattcgg gctccatgcc cttgtccggc cggctgaaga
     2221 tgccgccggc tttgaacatg atgaccttgc ggtccgggat ggaagacgcg tacagcgcct
     2281 gtgcgtcgcg atacgtgagc atggtcggtg ccagtgactt gcccgtctcg ggatccttct
     2341 gatccgagtc gtaggcgttg tcggggtccc agctgtcgat cgacgcggcg tacaggatgc
     2401 cggcgcgttc gccggccggg ttgtgagccg cggcgcggaa actcgtgagc gtgatcatgc
     2461 acacggagat accgagcgcg atggcgaaca ccatgagtgc ggtgagcccc ggatttcgcc
     2521 ggaagctctg cacggccagt cgcaagtaat agccgaacat gactcaggct ccggcgaggg
     2581 cggtgacgtg ggccgtagcg ccgtggggag gagcggccga gggttcgtag ggcttgaagt
     2641 cggagatctg gccatcgacc acctgcacgt tgcggtgcgc gcggcgggcg agttcggggt
     2701 cgtgagtcac catgatgatg gtggtgccca tctcgttgat gcgctccagc agatccatga
     2761 cctggcgggc catgagcgag tcgaggttgc ccgtgggttc gtcggcgaga acgagcttgg
     2821 ggtcgccggc gatcgcgcgg gcgatggcca cacgctgctg ctggccgccg gacaactggg
     2881 ccgggacgtg tttcatgcgc gcggccaggc cgacgatctc gagcgcgttg gtgatgcgct
     2941 tgtcgcgttc cgcggacttc atgcggcggt agcgcaaggg cacgtccacg ttgtcgtaga
     3001 cgttgaggtc gggcatcaga ttgaagctct ggaatatgaa tccgattttc tgattgcgca
     3061 ggtgcgaccg cgcgtcgtcg gataacgccg tgacctcgac gtcatcgagc ttgtagctgc
     3121 ccgtctcgta gggttcgagc agaccggcga tgttgaggaa cgtcgtcttg ccggagccgg
     3181 aaggtccggt gaccgcgaga aactcccccg ccttcacctg cagcgagaaa ttgcgcagcg
     3241 catgagtttc gatgaggtcg gtgcgaaaaa ctttgctgac gttggtcatg gagagcatgg
     3301 agattcttcc tcttagttac cgatgaggac gctgggcgcg tccttgtagt cgcgcatgtc
     3361 ggagagcacc acggtgtcgc cgacattgag tccgttgcgt atttcgattt cgccgatggc
     3421 cgccgcgccg aattccacgg gggtgcgcac cgcatcgtcg tcacgcacca cgtagatgaa
     3481 gcgcgtgtcg gaatcgttga aggggctgcg ggccaccttc agcacgtcgt tgcgttcgtc
     3541 gagcacgatg cgcaccgagg cgcgctggtt ctgccgcatg ccctggggct gttcggcaaa
     3601 gcgcacccgg ccagtgacct gggcattgcg tacgtcgggt gaaatgccgg cgacggttcc
     3661 gacgagcttg cggccatcga gcgtgatctc ggcattcatg cccgaccgga tctcgttggc
     3721 gtagctctcg gccacctgga actcgatctc gagcgccgtg aggtcgacca ccgtgaccag
     3781 cggcgc
//
LOCUS       DATDEU010000184         2778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_322995, whole genome shotgun sequence.
ACCESSION   DATDEU010000184 DATDEU010000000
VERSION     DATDEU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322995"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VIV63_04500"
     CDS             complement(<1..283)
                     /locus_tag="VIV63_04500"
                     /inference="COORDINATES: protein motif:HMM:NF037334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123889.1"
                     /translation="MRFTAWMPGALALVALALPAGAKEKLRYMEKLDRGLVAVQQSDG
                     KVFLSWRLLASDPDGVAFNVYRETERPATAADPGRFAPASHTAQTGPVRV"
     gene            complement(280..1770)
                     /locus_tag="VIV63_04505"
     CDS             complement(280..1770)
                     /locus_tag="VIV63_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6123890.1"
                     /translation="MNTPARRVHFGHYLAYGSNDFLGAGAMSIIGFWILFFYTTFCGL
                     SATQATIIFATARLLDAFFSPVIGYISDRFHNTWLGKKFGRRRFFILLAIPLVPSFAL
                     MWVEGQSFWYYLITYCFFELVYAMEIIPYETLAAEMSPNYQTKAKFAGARILCGQIAN
                     IAAMWLPGVIIAQLGGKESGATFLYLGVIFSVFFVFVALAVFLFTWERPREEIASIAP
                     RDAGGSPFHVITDLFQELWATLRIRAFRLHLGMYIGGYISQDVLNAVLSYVIAFIFLG
                     SVATASSITTWMACAQLVSVGLAIWLTLRIHAAASYRIALGFFSLGILGFAALYFSGV
                     ADIRWFLAVAVIAGLGRGALNFIPWSVYNYMPDVDEIVTGRRREGAFAGVMTFIRKLL
                     QSAVVFAVGPILDASGLLRSVKIQPVHVVNTAVTIMLIGSLGLMIFGFLVSLRFRLNR
                     DTHTVLMDEIDRFKTRPGTAPTPENRAIVEDLTGWKYEELWGKGRS"
     gene            complement(1767..>2778)
                     /locus_tag="VIV63_04510"
     CDS             complement(1767..>2778)
                     /locus_tag="VIV63_04510"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEY6123891.1"
                     /translation="EFLLRLPDGRVIDTKGWNDWEWTNGVGLYGIYKYYQMTGDARCR
                     EIMLNWFRDRFAAGTPTKNINTVAPFLTLAYLYEESPDSSWLPYLDEWAEWLMDGLPR
                     TEENGFQHIVFNSENREQLWDDTLMMSVLPLAKIGKLLGRPQYVEEARRQFMLHIKYL
                     VDRKSGLWFHGWTFDGRHHFANALWARGNCWVTIAIPEFIELLDLPPGDALRDFLLET
                     HLQQIRTLVKYQDAGGLWHTLIDDPKSYLEASAAAGFAYGILKSVRKGYIDAKYEEAG
                     VRAVKAVLANIDKAGELKNVSFGTPVFNDLDGYRNIPLTSMPYGQAMAILALGEFKRA
                     FI"
BASE COUNT          545 a          846 c          900 g          487 t
ORIGIN      
        1 tgacgcgtac gggtccggtc tgcgcagtgt gggacgcggg agcgaagcgc ccgggatcgg
       61 cggcggtcgc gggtcgctcc gtctcgcgat acacgttgaa tgcgacgccg tcaggatcgc
      121 tcgcgagcag ccgccagctc aggaaaacct tgccatcgct ctgctgtact gcaacgagac
      181 cgcgatcgag cttttccata tagcgcagtt tttccttcgc gccggcgggc aacgcgagcg
      241 ccacgagcgc gagcgcgccc ggcatccatg ccgtgaatct catgacctgc ctttccccca
      301 gagttcttcg tatttccatc ctgtgaggtc ctcgacgatc gcgcggttct ccggagtggg
      361 cgcggtgccg ggcctggtct tgaaacgatc gatctcgtcc atgagcacgg tgtgcgtgtc
      421 gcgattgagc ctgaagcgca ggctcacgag gaagccgaag atcatgaggc ccaggcttcc
      481 gatcagcatg atcgtcaccg cggtgttcac cacgtgcacc ggctggatct tgacgctgcg
      541 gaggagcccg ctcgcatcga ggatcggacc cacggcgaac accaccgcgg actgcagcag
      601 cttgcggatg aacgtcatga caccggcgaa tgccccttcg cgccgccggc ccgtcacgat
      661 ttcatccacg tcgggcatgt agttgtagac gctccacggg atgaagttca gcgcgccgcg
      721 cccgaggccg gcgatgaccg ccacggccag gaaccagcgg atgtcggcca cacccgagaa
      781 ataaagcgcg gcgaacccca ggatgccgag gctgaagaat cccaacgcga ttcgatacga
      841 agccgcggcg tgaatgcgca gcgtgagcca gatcgcgaga ccgaccgaaa ccagctgcgc
      901 gcacgccatc cacgtcgtga tcgatgaggc cgtggcgacc gaccccagga agatgaatgc
      961 gatgacgtac gacaacacgg cgttcagcac gtcctggctg atgtatccgc cgatgtacat
     1021 gccgaggtgc aggcgaaacg cccggatgcg cagcgttgcc cagagttcct ggaacagatc
     1081 ggtgatcacg tggaacggcg aaccgccggc gtcgcgaggc gcaatgctcg cgatctcctc
     1141 gcgcggccgc tcccaggtga acaggaacac ggcgagcgcc acgaacacga agaacaccga
     1201 gaagatcacg cccaggtaga ggaacgtggc gcccgactcc ttgccgccga gctgcgcgat
     1261 gatcacgccc ggcagccaca tcgcggcgat gttcgcgatc tgcccgcaga ggatgcgcgc
     1321 gcccgcgaac ttcgccttgg tctggtagtt gggcgacatc tccgcggcca gtgtttcgta
     1381 cgggatgatc tccatcgcgt atacgagctc gaagaaacag taagtgatca ggtagtacca
     1441 gaagctctgg ccttcaaccc acatgagcgc gaagctcggc acgagcggga tcgccagcag
     1501 gatgaagaac cggcggcggc cgaacttctt ccccaaccac gtgttgtgga agcggtcgga
     1561 gatgtagccg atgaccggtg agaagaacgc gtcgagcagg cgcgcggtcg cgaaaatgat
     1621 cgtggcctgg gttgcgctga gtccgcagaa cgtcgtgtag aaaaacagga tccagaagcc
     1681 gatgatcgac atcgcaccgg cgcccaggaa gtcgttgctt ccataggcga ggtagtgccc
     1741 gaagtgaacc ctgcgcgctg gcgtgttcat atgaacgcgc gtttgaattc gccgagcgcg
     1801 aggatggcca tggcctggcc gtagggcatc gaggtcaacg gaatattccg atagccgtcc
     1861 aggtcattga acaccggcgt gccgaaggaa acattcttga gctcgccggc cttgtcgatg
     1921 ttggcgagca ccgctttgac ggcgcggact cccgcctctt cgtatttcgc gtcgatgtaa
     1981 cccttgcgaa cggacttcag gatgccgtag gcgaagcccg cggcggcgct ggcctcgagg
     2041 taggacttcg gatcgtcgat gagcgtgtgc cagagtccgc ccgcgtcctg gtatttcacc
     2101 agcgttcgga tctgctgaag gtgggtttcg agcaggaagt cgcgcaatgc atcgccaggc
     2161 ggaaggtcga gcagttcgat gaactctgga atggcgatgg tcacccagca atttccacgg
     2221 gcccacagcg cgttggcgaa atgatgccgg ccatcgaacg tccagccgtg gaaccacagg
     2281 ccggacttcc ggtcgaccag gtacttgatg tgaagcatga actggcggcg cgcttcttcc
     2341 acgtactgcg ggcggcctaa caacttgccg atcttggcga gcggcagcac gctcatcatc
     2401 aacgtgtcgt cccagagctg ctcgcggttc tcggagttga agacgatgtg ctggaagccg
     2461 ttttcctcgg tgcgcggcag gccgtccatg agccactccg cccactcgtc gaggtacggc
     2521 agccaggagc tgtcggggct ctcttcgtac agataggcga gcgtgaggaa cggcgcgacc
     2581 gtgttgatgt tcttggtggg agtgccggcg gcgaaacggt cgcggaacca gttcagcatg
     2641 atctcgcggc accgcgcgtc gccggtcatc tggtagtact tgtagatgcc gtacaggccg
     2701 acgccgttcg tccattccca gtcgttccag cccttggtgt cgatgacgcg gccatcgggc
     2761 aggcgcagca ggaattcg
//
LOCUS       DATDEU010000185         2852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1198961, whole genome shotgun sequence.
ACCESSION   DATDEU010000185 DATDEU010000000
VERSION     DATDEU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198961"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..829
                     /locus_tag="VIV63_04515"
     CDS             26..829
                     /locus_tag="VIV63_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6123892.1"
                     /translation="MATKSAPPRQPAPESPSRTSRYRAPALEKGLDVIELLAAEKSPL
                     NLSAISQRLGRSSGELFRMLQVLEFKGFITTSENGTGYVLTNKLFALAMAQAPVRSLV
                     ETALPIMRKLVQDIGQSCHIAVASEDQIVVITRIERPGDLGFSVRIGYRREIATATSG
                     LVLFAYQNEEVRRIWLKRCRLKGEAAESFVQRADQVRSRGHHEAASHFVRGIVDLSAP
                     ILRGDTAVAALTIPFVHSNPLQVEMAQAVEFVRAAAKQISSEIVHGDLP"
     gene            826..2115
                     /gene="fucP"
                     /locus_tag="VIV63_04520"
     CDS             826..2115
                     /gene="fucP"
                     /locus_tag="VIV63_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEY6123893.1"
                     /translation="MNDATTSKSTSFITSGYLFATILIVGLFFMWGVANSLNDVLIPQ
                     FRKTFQLDDFASSMVQSAFYFGYFCFAIPASLFMRRFGYRAAVLMGLGLYAAGALLFL
                     PAAQASQYYAFLIALYVIASGLAFLETSANPLIAVMGPAGSADQRLNFAQTFNPIGTM
                     AGSTIGGLLIFSDVNYTPEQLQALTPEAFAAFRATELDAVKLPYICIAAMVILWALMV
                     SVAKFPPVDRGRGADGEKEGGFRGLSKFPRYWLGVLAQFAYVGAQVGVWSFLIRYTQH
                     NFPGTLEKDALRWVPISFGVFFAGRLLGTLLMSRVNPAKLLASFAAINVVLCLVGAMA
                     GGLVGLTALIASSFFMSIMFPTIFSMSLRGLGIYTKSGSSFLVMAIIGGAVFTALMGY
                     ISRESSINLAYVVPAACFAVVLLFAWKSRRDTVTAIA"
     gene            2112..>2852
                     /locus_tag="VIV63_04525"
     CDS             2112..>2852
                     /locus_tag="VIV63_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6123894.1"
                     /translation="MRRRELGRTGLEVSELGFGGAAIGNLYRELADDDASAAVRESFA
                     AGVRYFDTAPFYGFGLSESRLGQALNGAQALPVISTKVGRRLVPTGPQDASVGREGYF
                     SPRPFAPVFDYGYESVMRSHAESLERLQVPRVDILLCHDIGHMTHGNAHAARVAEFLD
                     GGYRAMRELRDAGVVRAIGLGVNESEICVELLASREFDCILLAGRYTLLEQPALAALL
                     PLCEERNVSIICGGPFNSGGLAAGSRAGA"
BASE COUNT          442 a          962 c          883 g          565 t
ORIGIN      
        1 cattaccact agaactacag aatccatggc caccaaatcc gctcccccgc gccagccggc
       61 gccggaaagt ccctcgcgca ccagccgcta ccgtgccccg gcgctcgaga aaggtctcga
      121 cgtcatcgag ctgctggcgg cggagaagtc cccgctcaat ctatccgcca tatcgcagcg
      181 cctcggacgc tcgtcgggcg agctgtttcg catgttgcag gtcctcgagt tcaaggggtt
      241 catcaccacg tccgagaacg gcaccggcta cgtgctcacg aacaagctgt tcgccctcgc
      301 catggcccag gcgccggtgc gatccctggt cgaaaccgcg ttgcccatca tgcgcaagct
      361 ggtccaggac atcggccagt cctgccacat cgcggtggcg tccgaagacc agatcgtggt
      421 gatcacgcgc atcgagcggc ccggcgatct cggcttctcc gtccgtatcg gctaccggcg
      481 cgagatagcg accgccacct cagggctggt cctcttcgcc taccagaacg aagaagtgcg
      541 ccgcatctgg ctcaagcgct gccgactcaa gggcgaagcc gccgaatcct tcgtgcagcg
      601 cgccgaccag gtgcgctcgc ggggccacca cgaggcggcg tcgcatttcg tacgcggcat
      661 cgtcgatctg tccgcgccca tcctgcgcgg cgatacggcg gtcgcggccc tcaccattcc
      721 attcgtccac agcaatccgc tgcaggtcga gatggcccag gcggtggagt tcgtgcgcgc
      781 cgcggccaag cagatatcca gtgaaatcgt gcatggagat ctgccatgaa cgacgcgacg
      841 accagcaagt ccacgtcatt catcacctcg ggctatctct tcgcgacgat cctcatcgtc
      901 ggactgtttt tcatgtgggg cgtcgccaac agtctcaacg acgtgctgat cccgcagttc
      961 cgcaagacgt tccagctcga cgacttcgcc tcgagcatgg tgcagtcggc gttctacttc
     1021 ggctatttct gtttcgcgat tcccgcgtcg ctgttcatgc ggcgtttcgg ctaccgcgcc
     1081 gcggtgctca tgggcctcgg cctgtacgcc gccggcgcgc tgctgttcct gcccgccgcg
     1141 caggctagcc agtactacgc gttcctcatc gcgctgtacg tgatcgccag cggcctcgca
     1201 tttctcgaga catcggcgaa tccgctgatc gcggtcatgg ggccggcggg cagcgccgac
     1261 cagcgcctga acttcgcgca gaccttcaat ccaatcggca cgatggccgg ctccaccatc
     1321 ggcggcctgc tcatcttctc cgacgtgaac tacacgcccg agcaattgca ggcgctgact
     1381 ccggaggctt tcgccgcctt tcgcgccacc gagctggacg cagtcaaact accttacatc
     1441 tgcatcgccg cgatggtgat cctgtgggcg ctcatggtct ccgtcgcgaa gtttccgccc
     1501 gtggatcgcg ggcgcggcgc ggatggcgaa aaggaaggcg gcttccgcgg cctgtcgaag
     1561 tttccgcgtt actggctggg cgtcctcgcg cagttcgcct acgtgggcgc gcaggtgggc
     1621 gtctggagtt ttctcatccg ctacacgcag cacaattttc ccggcacgct ggaaaaggat
     1681 gcgcttcgct gggtgccgat ctctttcggc gtcttctttg ccggccgatt acttgggacc
     1741 ttgctgatgt cacgcgtgaa tccggcaaag ctgctggcca gtttcgccgc gatcaacgtg
     1801 gtgttgtgcc tggtcggcgc catggcgggc ggactcgtgg gcctgaccgc cctcatcgct
     1861 tccagcttct tcatgtccat catgttcccg accatcttct ccatgagcct tcgcggcctc
     1921 ggcatctata cgaagtcagg ttcgtcgttc ctggtgatgg ccatcatcgg tggcgcggtg
     1981 ttcaccgcgc tcatggggta catctcgcgc gagagctcga tcaacctcgc gtatgtcgtg
     2041 ccagccgcct gtttcgcggt ggtgctgctg ttcgcctgga agtcgcggcg agacacggtg
     2101 acggctatcg catgaggcgt cgcgaactgg gcaggaccgg gctcgaagtc agcgagctgg
     2161 gtttcggtgg cgccgccatc ggcaacctgt atcgcgaact ggccgacgac gatgcgagcg
     2221 ccgcggtgcg cgagtcgttc gccgccggcg ttcgctactt cgacaccgca cccttctatg
     2281 gtttcgggct cagtgaatcg cggctcggac aggcgctgaa cggcgcacag gccttgccgg
     2341 tgatctccac gaaggtggga cggcgtctcg tgcccaccgg accgcaagac gccagcgtgg
     2401 gacgtgaagg ctatttttcg ccacgtccct tcgcgccggt gttcgactac ggttacgaat
     2461 cagtcatgcg ttcgcatgcc gagagtctcg aacgtctgca ggtgcctcgc gtcgacatcc
     2521 tgctgtgcca cgacatcggg cacatgaccc atggcaatgc gcatgcggcg cgcgtcgcgg
     2581 aatttctcga cggcggctac cgcgcgatgc gcgagctgcg cgatgcgggc gtggtgcgcg
     2641 ccatcggcct cggtgtcaac gaatcggaga tctgcgtcga attgctcgcg tcgcgcgaat
     2701 tcgactgcat cctgctggcc ggccgctaca cgctgctcga acagccggcg ctggccgcgc
     2761 tgctgccgct gtgtgaggag cgcaacgtct ccatcatctg cggagggccg ttcaactcgg
     2821 gcggtctcgc cgccgggtcg cgcgccggcg cg
//
LOCUS       DATDEU010000186         4229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1491244, whole genome shotgun sequence.
ACCESSION   DATDEU010000186 DATDEU010000000
VERSION     DATDEU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491244"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2379
                     /locus_tag="VIV63_04530"
     CDS             <1..2379
                     /locus_tag="VIV63_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6123895.1"
                     /translation="EVVRLRDIARLELGAATYAMQSLLGNENAVAIPVYQSPGSNALE
                     LSTKVRADMETLKKSFPQGMDYKIIYDPTQFVRQSIDAVVETLLEAVLLVVLVVILFL
                     QTWRASIIPLVAVPVSVIGTFAVLYALGFSINTLSLFGLVLAIGIVVDDSIVVVENVE
                     RHIANGLKPLEATKKAMGEVSRPIIAITLVLCAVFVPVAFVEGLTGQFYRQFALTIAI
                     STVISAFNSLTLSPALAAVLLKPHGAKPDALTRGMDKVFGRVFARFNKMFTSASDAYS
                     RGAAKTVKRAPIAIAIYAGLIALTVLGFYKTPAGFIPQQDKLYLVGVVQLPPAASIDR
                     TAEVVRRMGEIAKQEPGVLDSVQFGGVSANGFAAQSSAALVFFPLKDFSEREGLSAGA
                     IAGALNQKFAGIQDAYIAVFPPPPVIGLGMLGGFKLNVQDRQNRGAEALYAAVQDALG
                     KAWQDPQLAGVFSSYQINVPQLDVDVDRLKVKRQGVNLNDVFQTLQVNLGSLYVNDFN
                     RFGRTYRVVAQADAPFRSQVDDILPLKTRNAAGEMVPLGSMISVKEAFGPDIVERFNG
                     YASADINGGPSPGHSSGESQAAISKILDENLPAGMTYEWTDLAFQQIDAGDAALFVFP
                     LCVLFVFLVLAAQYESFSLPLAIILIVPMALLAGLVGIYLQGGDNNIFTQIGFLVLVG
                     LASKNAILIVEFAKHLQEEGRDRVSAVLEAARLRLRPIVMTSIAFIMGVLPLVLADGA
                     GAEVRRAMGAAVFSGMIGVTVFGLLLTPVFYVLLRRDRKVEPAQLEEVSTHA"
     gene            2372..3784
                     /locus_tag="VIV63_04535"
     CDS             2372..3784
                     /locus_tag="VIV63_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY6123896.1"
                     /translation="MRKARFLLAPIAAAVLAACAVGPNYHAPETKAAEKFDNVEATYS
                     TEQGTAQFWQSFADAELDKLVSEALASNHDLRIALTRVKEARALRRDAAFDLAPSINA
                     SGGYTKTRTSSEGRLPGISRETELYDAGFDAFWELDFFGGVRRGLEASRAELGAEEAA
                     LQDVQVIVTAEVTRAYFELRGQQLQLDVARRNVANQQSTLDLAQVRLDAGSGTEFDTA
                     RAQAQLSATLGTIAPLEAAVARSIHRLSVLVGREPGALRAQLAPAQTLPPLPGIVPVG
                     DPAGLLRRRPDIRVAERELASATARIGVAVADLFPRVTFTGSAGYVASSSSALGDNGT
                     DAYVLAPGISWGIFDLGHVQARIGAAKWRKEGAFLRYEQTVLQALEETEDSLVTHARA
                     RDRLVHDEAAVRASNTAAGLARVRYENGASDFLQVLDAERTLLESEDRLARSRTEAAT
                     SLIAVYKSLGGGWEGAPHPN"
     gene            3826..>4229
                     /locus_tag="VIV63_04540"
     CDS             3826..>4229
                     /locus_tag="VIV63_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123897.1"
                     /translation="MSINPVDLAAAAEGILNTQYLLQRERLLSASAQASRLLLEAPDA
                     MKAMPEVLRLLGEAAQVDRTALAFAEIGPNGEKWLKIQAEWVTESLSDTCCPIAAGEC
                     TDPWNEPRSDQFCTMLQAGKSVVFCPDAARAI"
BASE COUNT          690 a         1461 c         1344 g          734 t
ORIGIN      
        1 gaagtcgtgc gtctgcgcga catcgcgcgc ctggagctcg gcgcggccac gtatgcgatg
       61 caatcgctgc tcggcaacga aaacgccgtg gccattccgg tctaccagtc tccgggttcg
      121 aacgcgctcg agctttccac gaaagtccgc gccgacatgg aaacgctcaa gaagagtttt
      181 ccccagggca tggactacaa gatcatctac gacccgacgc agttcgtgcg ccagtccatc
      241 gacgccgtgg tcgagacctt gttggaggcc gtgctgctgg tcgtgctcgt cgtcatcctg
      301 ttcctgcaga cctggagagc ctcgatcatt ccgctggtcg ccgtgccggt ctcggtcatc
      361 ggcaccttcg ccgtgttgta cgcgctcggc ttctccatca atacgctctc gctgttcggt
      421 ctcgtgctgg ccatcggtat cgtggtcgac gactccatcg tggtcgtcga gaacgtcgag
      481 cggcacatcg ccaatggact gaaaccgctc gaggccacca aaaaggccat gggcgaagtc
      541 agccgcccga tcatcgccat cacgctggtg ttgtgcgcgg tgttcgtccc ggtcgccttc
      601 gtcgaaggtc tcaccggcca gttctatcgc cagttcgcgc tgaccatcgc catctcgacg
      661 gtgatctccg cgttcaactc gctgacgctg tcgccggcgc tcgccgcggt gctgctcaag
      721 ccgcacggcg ccaagcccga cgcgctcacg cgcggcatgg acaaggtgtt cggtcgcgtg
      781 ttcgcgcgtt tcaacaagat gttcacgtcg gccagcgacg cctactcgcg cggcgccgcg
      841 aagaccgtca agcgcgcgcc gatcgccatc gcgatctacg ccggattgat cgcgctcacg
      901 gtactgggct tctacaagac gcccgcgggt ttcatcccgc agcaggacaa gctgtatctc
      961 gtcggcgtgg tccagttgcc acccgccgcg tccatcgatc gcaccgcgga agtcgtgcgc
     1021 cgcatgggcg agatcgccaa gcaggagccc ggcgtcctcg actccgtgca gttcggcggc
     1081 gtgtcggcca acggcttcgc cgcgcagtcg agcgccgccc tggtgttctt cccgctcaag
     1141 gatttctcgg agcgtgaagg cctttccgct ggcgccattg ctggtgcact gaaccagaag
     1201 ttcgcgggta tccaggatgc gtacatcgcg gtgttccccc cgcctccggt catcggcctc
     1261 ggcatgctgg gcggtttcaa gctcaacgtg caggaccgtc agaaccgcgg cgcggaggcg
     1321 ttgtacgccg ccgtgcagga cgcgctcggc aaggcctggc aggacccgca gctcgccggc
     1381 gtgttctcca gctaccagat caacgttccg cagctcgacg tcgacgtgga ccgcctcaag
     1441 gtcaagcgcc agggcgtgaa cctcaacgac gtcttccaaa cattgcaggt gaacctgggg
     1501 tccctctatg tcaatgactt caaccgattt ggccgtactt accgtgtggt cgcacaggcg
     1561 gatgcgccgt tccgttcgca ggtggatgac atccttcctc tcaagacgcg caacgccgcc
     1621 ggagaaatgg tgcccctcgg ctcgatgatc tccgtcaagg aagcgttcgg cccggacatc
     1681 gtcgaacgtt tcaacggcta cgcgagcgcc gacatcaacg gtggtccgtc gccgggtcac
     1741 agctcgggtg agtcgcaggc ggccatctcg aagatcctcg acgagaacct gcccgccggc
     1801 atgacctacg aatggaccga cctcgcgttc cagcagatcg acgcgggtga tgcggcactg
     1861 ttcgtcttcc cgctgtgcgt gttgttcgtg ttcctggtgc tggccgcgca gtacgagagc
     1921 ttctcgctgc cgctcgccat catcctgatc gtgccgatgg cgttgctcgc cggcctcgtg
     1981 ggtatctatc tacagggcgg cgacaacaac atcttcacgc agatcgggtt cctggtgctc
     2041 gtcggcctcg cgtccaagaa cgccatcctg atcgtggagt tcgccaagca cctgcaggaa
     2101 gaaggccgtg accgcgtgtc ggccgtgctc gaagccgcgc gcctccggtt gcgccccatc
     2161 gtgatgacgt ccatcgcgtt catcatgggc gtgttgccgc tcgtgctggc cgacggcgcc
     2221 ggcgcggaag tgcgccgcgc catgggcgcg gcggtgttct cgggaatgat cggcgtaacc
     2281 gtgttcggcc tgctgctgac tcccgtgttc tatgtgttgc tgcgccggga ccggaaggtc
     2341 gagccggcgc agctcgaaga ggtttccacc catgcgtaag gcccgattcc tgttagcccc
     2401 gatcgcagcg gccgtactgg ccgcctgcgc ggtgggcccc aactaccacg cgccggaaac
     2461 gaaggccgcg gagaaattcg acaacgtcga agccacctac tccaccgagc agggtaccgc
     2521 gcagttctgg cagagctttg ccgatgccga actcgacaag ctcgtgagcg aggcgctggc
     2581 ctcgaaccac gatctgcgca tcgcgctcac gcgtgtgaag gaagcccgcg cactgcgccg
     2641 cgacgccgcc ttcgatctcg cgccgagcat caatgccagc ggtggttaca cgaagacgcg
     2701 cacctcgtcc gaaggacggc tgcccggcat ttcgcgtgaa acggaactct acgacgcggg
     2761 cttcgatgcc ttctgggaac tcgacttctt cggcggtgta cgccgcgggc tcgaagccag
     2821 ccgcgccgaa ctcggcgccg aggaagccgc gttgcaggac gtgcaggtga tcgtcacggc
     2881 cgaagtcacg cgcgcctatt tcgaactgcg cgggcagcaa ctgcagctcg acgtggcccg
     2941 gcgcaacgtc gccaatcagc aatccacgct cgatctcgcc caggtacgtc tcgatgccgg
     3001 cagcggaacc gagttcgaca ccgcgcgcgc gcaggcgcag ttgagcgcga cgctcggcac
     3061 catcgcaccg ctcgaggcgg ccgtcgcgcg ctccatccat cgcctctcgg tgctcgtggg
     3121 ccgcgagccc ggcgcactgc gcgcccaact cgcccccgcg caaaccctgc cgccgctgcc
     3181 cggcatcgtg ccggtaggcg acccggcagg cctcctgcgc cgccgcccgg acatccgggt
     3241 cgccgagcgc gaactggcga gcgccaccgc gcgtatcggc gtcgccgtgg ccgacctgtt
     3301 cccgcgtgtc acgttcaccg gcagcgcggg ctacgtcgcc agctcgtcga gcgcgctggg
     3361 cgacaacggc accgacgctt acgtgctggc gcccggcatc tcctggggca tcttcgacct
     3421 gggccacgtg caggcgcgca tcggcgcggc caagtggcgc aaggaaggcg ccttcctgcg
     3481 ttatgagcag accgtgctcc aggcgctcga ggaaactgag gattcgctgg tcacccacgc
     3541 ccgcgcccgg gaccgcctgg tccatgacga ggcggccgtg cgcgccagca acaccgcggc
     3601 gggcctggcc cgggtacgtt atgaaaacgg cgccagcgac ttcctgcagg tgctggatgc
     3661 cgagcggacg ctgctcgagt ccgaggatcg cctcgcccgc agccgtaccg aagccgccac
     3721 cagcctgatt gccgtgtaca agtcgctcgg cgggggttgg gagggagcgc cccatcccaa
     3781 ttgagggagg gaaatcaaag ccttccacga gtaaagtgca tgtccatgag catcaatccc
     3841 gtagacctcg ccgccgcggc cgagggcatc ctcaacacgc aatacctgct ccagcgcgag
     3901 cggctgctgt cggcctcggc gcaagccagc cggctgctgc tcgaagcgcc cgacgccatg
     3961 aaggccatgc ccgaggtgct ccggttgtta ggcgaggccg cgcaggtcga ccgtaccgcg
     4021 ctcgcgttcg ccgagatcgg ccccaacggt gagaagtggc tcaagatcca ggccgagtgg
     4081 gtcacggaat cgctgagcga cacctgctgc cccatcgccg ccggtgagtg cacggacccg
     4141 tggaacgagc cgcgctcgga ccagttctgc accatgttgc aggccggcaa gagcgtggtc
     4201 ttctgccccg acgccgcacg cgccatcga
//
LOCUS       DATDEU010000187         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_323623, whole genome shotgun sequence.
ACCESSION   DATDEU010000187 DATDEU010000000
VERSION     DATDEU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323623"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VIV63_04545"
     CDS             complement(<1..746)
                     /locus_tag="VIV63_04545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; sigma 54 factor is
                     responsible for the expression of enzymes involved in
                     nitrogen assimilation and metabolism; the rhizobia often
                     have 2 copies of this sigma factor; in Rhizobium etli
                     RpoN1 shown to be involved in the assimilation of several
                     nitrogen and carbon sources during free-living aerobic
                     growth and RpoN2 is involved in symbiotic nitrogen
                     fixation; in Bradyrhizobium both RpoN1 and N2 are
                     functional in free-living and symbiotic conditions, rpoN1
                     gene was regulated in response to oxygen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY6123898.1"
                     /translation="MMKPSLQLKLGQTLTMTPQLQQAIRLLQMPALELQAHIREQLES
                     NVMLEPVDEYEATGTFETLEPHPMPEEKRVDAQAAPEVTVEAEVIEDNWSERSAGSDS
                     WRDDDERAAEVADDAQLSLQDHLVGQLEYANLAPRDLAIARAIVDAINDDGYLTDSGD
                     DIAATLLPEVTADADDVERVLRLVQTLDPAGVAARNLGECLALQLRTLHPDTPGLRLA
                     LAIADKHLDKLADRELTSLRRELAASDDELA"
     gene            complement(811..1533)
                     /gene="lptB"
                     /locus_tag="VIV63_04550"
     CDS             complement(811..1533)
                     /gene="lptB"
                     /locus_tag="VIV63_04550"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY6123899.1"
                     /translation="MSILRATHLAKAYKKRQVISDLSVEVESGQIVGLLGPNGAGKTT
                     AFYMIVGLVPPDRGTVTIDDRDITHLAMHQRAHAGIGYLPQEASVFRKLTVEQNVMAI
                     LETRDLDDTARQERLEQILGELRIAHVRKSLGMALSGGERRRVEIARALAAEPRFVLL
                     DEPFAGVDPLSVIDIQRIMNELTAKGIGVLITDHNVRETLGVCQRGYVVSGGTVMASG
                     TPEEILANPQVREVYLGQSFRM"
     gene            complement(1544..2203)
                     /gene="lptA"
                     /locus_tag="VIV63_04555"
     CDS             complement(1544..2203)
                     /gene="lptA"
                     /locus_tag="VIV63_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY6123900.1"
                     /translation="MATSNRIKLIAVSGALGACGLLGVAEGAAAPCVNQEIVLEAKPL
                     EMDYRTNNAVLRDVVITQCGMRIQAAEANIKGGLNFDDSKWTISGDVRITASGGSLTS
                     DKAIVSFRNKVISAATITGAPAQFEQQRDDGTTARGRANTIDYETSSGTVSFNTNAWL
                     SYGRNEITGQQLVYNIRTQSVQTQPKPGAARGDGRIRLVIQPDKPPQLPVPEKEKEKK
                     P"
BASE COUNT          346 a          677 c          759 g          460 t
ORIGIN      
        1 gcgagttcgt cgtcggaggc ggcgagttcg cggcgcagcg acgtgagctc gcgatccgcg
       61 agcttgtcga gatgtttgtc ggcgatcgcc agcgcgagac gcaggcccgg cgtatccgga
      121 tgcagcgtgc gcagctgcaa cgcgagacat tcgccgaggt tgcgcgccgc cactcccgcg
      181 gggtcgagag tctgcacgag gcgaagcacg cgctcgacgt cgtccgcgtc ggcggtgact
      241 tcgggcagca gcgtcgcggc gatgtcgtcg ccggaatcgg tgaggtaacc gtcgtcgttg
      301 atcgcatcga cgatcgcacg tgcgatggcg aggtcgcgcg gcgcgaggtt cgcgtattcg
      361 agctgaccga ccaggtgatc ctgcagcgag agctgcgcgt cgtcggcgac ttcggcggcg
      421 cgttcgtcgt catcgcgcca ggaatcggag cccgcgctgc gttcgctcca gttgtcttcg
      481 atgacttcgg cttcgaccgt gacttcggga gcagcctgcg cgtcgacgcg cttctcctcc
      541 ggcatcggat gcggttcgag ggtttcgaaa gtgccggtgg cttcgtattc gtccacgggc
      601 tcgagcatga cgttgctttc gagctgttcg cggatgtgcg cctgcagctc cagcgccggc
      661 atttgcagga ggcgaatcgc ctgctgcagc tgcggcgtca tggtcagcgt ctgacccagc
      721 ttcagctgca gggatggctt catcatcgac acaggcgtgg gtgtctcaac gctatgaaag
      781 aaaaagattt ttccgcagca tcgcggaacg ctacatgcgg aaagactgac ccaagtacac
      841 ctcacggact tgggggttgg caaggatttc ttcgggcgtt cccgaagcca tcacagttcc
      901 cccggaaact acgtaaccgc gctgacagac acctagggtt tctctgacgt tatggtcggt
      961 aatgagcacg ccgatgccct tggcggtgag ctcattcatg attcgctgga tatcgatgac
     1021 cgatagtggg tcgacacccg caaaaggctc gtcgagcagc acaaaacggg gctcggcggc
     1081 cagcgcacgc gctatttcga cgcgccggcg ctcgccgccc gacagcgcca tgccgaggct
     1141 cttgcgcacg tgcgcgatgc gcagttcgcc caggatctgt tcgaggcgct cctggcgcgc
     1201 ggtgtcgtcg aggtcgcgcg tctcgagaat cgccatgacg ttctgttcga cggtcagctt
     1261 gcggaacacc gacgcttcct gcgggaggta gccgatgccg gcatgcgcgc gctgatgcat
     1321 ggcgaggtgc gtgatgtcgc ggtcgtcgat ggtcacggtg ccccggtcgg gcggcacgag
     1381 tccgacgatc atgtaaaagg ccgtggtctt gcccgcgccg ttcgggccta acaacccgac
     1441 gatctgcccg ctctccactt cgacggagag atcggagatc acctggcgct tcttgtacgc
     1501 cttggcgaga tgagtggccc gcaagatgct caattctttg ggctcacggt ttcttctctt
     1561 tttctttttc gggcacggga agctgcggcg gtttgtcagg ctggatcacg aggcggatgc
     1621 gcccgtcgcc gcgggcggcg ccgggcttcg gctgcgtctg aacgctttgc gtgcggatgt
     1681 tgtagaccag ctgctgcccg gtgatttcgt tgcgcccgta cgagagccac gcgttggtgt
     1741 tgaaactcac ggtaccgctg ctcgtttcgt agtcgatggt gttggcccgg ccgcgcgcgg
     1801 tggtcccgtc gtcgcgctgc tgttcgaact gcgcgggcgc gccggtgatg gtggcggcgg
     1861 agatgacctt gttgcgaaac gagacgatcg ccttgtcgga ggtcaggctg ccgccgctgg
     1921 ccgtgatgcg cacgtcgccg gagatcgtcc acttgctgtc gtcgaaattg agtccgccct
     1981 tgatgttcgc ctcggcggcc tggatgcgca tgccgcattg cgtgatgacc acgtcgcgca
     2041 gcaccgcgtt gttggtgcgg tagtccatct ccaggggctt ggcctcgagc acgatttcct
     2101 ggttgacgca gggcgccgcg gcgccttcgg ccacgcctaa caacccgcag gcaccgaggg
     2161 cgcccgaaac ggcgatgagc ttaattcggt ttgaagtggc cattgacgtc cgattctaga
     2221 cgcagcgtgc cggccttcaa at
//
LOCUS       DATDEU010000188         3119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_177977, whole genome shotgun sequence.
ACCESSION   DATDEU010000188 DATDEU010000000
VERSION     DATDEU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177977"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VIV63_04560"
     CDS             <1..580
                     /locus_tag="VIV63_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123901.1"
                     /translation="LALFYMRRKDQQVATGEQLVPGDPLTFVQYTDNAAGGENYGLEG
                     TLQWRPVDPLLIDIRAAVLETRYIDYSNGERNLDGREQAHAPQYQYDLGLEYRGARGA
                     FVRVDFAGLDDFYFDDSHDERAPSRLLTHLKAGVSGKHWRAEVWVRNLFDRYYSQRGF
                     FFGNEPPDFTPTRYVQAGDPRHAGVTVTYTFR"
     gene            591..833
                     /locus_tag="VIV63_04565"
     CDS             591..833
                     /locus_tag="VIV63_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HEY6123902.1"
                     /translation="MNIGVEISLYPLHDDYIPPIRGFIERLNTDGRFKVLTNDMSTQV
                     FGQYEDVMDALTREIRPTFERDGKAIFVMKVLGPLG"
     gene            830..1420
                     /gene="pnuC"
                     /locus_tag="VIV63_04570"
     CDS             830..1420
                     /gene="pnuC"
                     /locus_tag="VIV63_04570"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEY6123903.1"
                     /translation="MSLSSEVLTAFEILAMITGVGYAVFAARRNRLCWVAGAISSGLA
                     AFLAGIRGLPMQSALQVFFVGMSAYGWMSWTRSAAKGELPVGLWPIPWHIGAALVVIL
                     LAFASAQVLAAETNAAWPLLDSLTTGFSLLATWLAARAKLENWIYWIAIDGVLVFLFY
                     AQDLPYLALLNVLFIGIAAGGFVAWRRRWHQQMVTA"
     gene            1417..2328
                     /locus_tag="VIV63_04575"
     CDS             1417..2328
                     /locus_tag="VIV63_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HEY6123904.1"
                     /translation="MIAGELLHNVPGCEDGEPPFSQELIGGGKVNRSFLVRTRRGRFV
                     VRLNENTTGDPGLDRDRELALHTVAAGAGIAPRVVYASPDRSSLITDFLDGRLWTPHY
                     FTRMRDLRSLGQRLRTLHSLTPPQVARFEPLNTARRYADIIVRSEPDEAGRIEFLLAN
                     GAEALTRTGGERRAPAVVHCDLHHGNVLTADRVYFIDWEYAQVGDPLIDLACIMAYYP
                     RALPHGALLLEAAGLTDAGVTPAMLGELTNVFTLLTYLWYRARRVARNVPATDLQIES
                     AALRRLLSLAPDAQPRHTSAANLRDSS"
     gene            2345..2659
                     /locus_tag="VIV63_04580"
     CDS             2345..2659
                     /locus_tag="VIV63_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6123905.1"
                     /translation="MATLQVIDRDGVSHDVECKPGLKVMEVLRELDYGVAAICGGMCS
                     CATCHIYVDPEWAGKLPPPMSDERDLLTELSHYRENSRLSCQVEITAALNGLKVTIAE
                     DE"
     gene            2677..>3119
                     /locus_tag="VIV63_04585"
     CDS             2677..>3119
                     /locus_tag="VIV63_04585"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEY6123906.1"
                     /translation="MLTSATWMYVCAIIGGVIAGWRIYQNLNKLRTRKNDTWDERLID
                     QLRKQGSDPFKPHDVDFFLAFPTKEGAEFLASRFKTEGFDSDVVDSPENGDLRYSLHL
                     HKSMLLVVPDMKELSRRLTDAAKEKEGRYDGWSAKPVARASVEPV"
BASE COUNT          548 a          995 c          991 g          585 t
ORIGIN      
        1 tctcgcactc ttttatatgc gccgcaagga tcagcaggtc gccaccggcg aacagctggt
       61 gcccggcgat ccgctgacct tcgtgcagta caccgacaat gcggcgggtg gcgagaacta
      121 cggcctggaa ggcaccttgc aatggcggcc cgtcgatccc ttgctgatcg acatccgcgc
      181 cgcggttctc gagacacgtt acatcgacta ctcgaacggg gagcgcaacc tcgacgggcg
      241 cgagcaggcg catgcaccgc aatatcaata cgacctgggc ctggagtatc gaggcgcgcg
      301 cggcgcgttc gtgcgcgtgg acttcgccgg cctcgacgat ttctacttcg atgattcgca
      361 cgatgaacgt gcgccgtctc ggctgctgac gcacctcaag gccggtgtct cagggaaaca
      421 ctggcgcgcg gaagtttggg tgagaaacct gttcgacagg tactattcgc agcgtggatt
      481 ctttttcggc aatgagccgc cggatttcac accgactcgt tacgttcagg cgggcgatcc
      541 gcgtcatgcg ggcgtgacgg tgacgtacac ttttcgctga cggagaacag atgaacatcg
      601 gcgtcgagat cagcctgtac cccttgcacg acgactacat accgccgatc cggggattca
      661 tcgaacgcct caataccgac ggccgtttca aggttctgac caacgacatg agcacgcagg
      721 tcttcggcca gtacgaagac gtcatggatg cgctcacgag ggaaatccgg cccacgttcg
      781 aacgcgatgg caaagccatt ttcgtcatga aggtcctggg ccctctcggt tgagcttgtc
      841 ttccgaagtg ctcacggctt tcgagatcct ggcgatgatc accggggtcg ggtacgccgt
      901 gttcgccgcg cggcgcaacc ggttgtgctg ggtggccggg gccatcagct caggcctggc
      961 tgccttcctc gcgggaatcc gcgggctgcc gatgcagtcg gcgctgcagg tctttttcgt
     1021 cggcatgtcg gcatacggct ggatgagctg gacgcgcagc gcggcgaagg gcgagttgcc
     1081 ggtggggttg tggcccattc cgtggcatat cggtgcggcg ctcgtggtca tattgctggc
     1141 gttcgcttcc gcacaggtgc tggccgctga gaccaatgcc gcctggccgc tgctcgattc
     1201 actaactacc ggcttcagcc tgctggccac gtggctcgcg gcgcgggcca agctcgaaaa
     1261 ctggatctac tggatcgcca tcgacggagt actggtgttc ctcttctacg cgcaagacct
     1321 gccatatctc gccttgctca acgtgctgtt catcggcatc gcggccggcg gtttcgtcgc
     1381 ctggcgccgg cgctggcacc agcagatggt gaccgcatga tcgccggaga gctgctgcac
     1441 aacgtgccgg gttgcgaaga cggggaaccg cctttctcgc aggagctcat cggcggcggc
     1501 aaggtcaacc ggagtttcct ggtgcgcacg cgccgcggcc gtttcgtcgt gcgtctcaac
     1561 gaaaacacca ccggagatcc gggcctggat cgcgatcgtg agcttgcgct gcacacggtc
     1621 gcagcgggcg cgggcatcgc gccgcgagtg gtctacgcct ctcccgaccg ctcgagcctg
     1681 atcaccgact ttctggacgg gcgcctgtgg acaccgcatt acttcacgcg catgcgtgat
     1741 ctgcgttctc tcgggcagcg cctgcgcacg ttgcattcgt tgacgccgcc gcaggtggcg
     1801 cgattcgagc ccctgaacac cgcacgccgt tatgccgaca tcatcgtccg cagcgaaccg
     1861 gacgaagcag ggcgcatcga gttcttgtta gccaatggcg ccgaagcgct gacgcggacc
     1921 gggggcgaaa ggcgcgcgcc ggccgtcgtg cactgcgacc tgcaccacgg caatgtgttg
     1981 accgccgacc gcgtgtactt catcgactgg gaatacgcgc aggtgggcga cccgctgatc
     2041 gaccttgcct gcatcatggc gtattacccg cgcgcgttgc cgcacggtgc gctgctgctc
     2101 gaagcggccg ggctcaccga cgccggtgtc acaccggcca tgctgggcga gctcaccaac
     2161 gtattcacgc tgctgacata tctctggtac cgcgcgcgcc gcgtggctcg caacgtgccc
     2221 gccaccgacc tgcagatcga atcggcggcc ctgcggcgcc tgctttcgct ggcgcccgac
     2281 gcgcaacctc ggcacactag cgccgcgaat ttgcgggact cttcttaaag ttttgggaac
     2341 tggaatggcg acgctgcagg tgattgatcg ggacggtgtt tcccacgatg tggagtgcaa
     2401 gccgggtctg aaagtgatgg aggtgttgcg cgagctggac tacggcgtcg cggccatctg
     2461 cgggggcatg tgctcctgcg ccacctgcca catctacgtc gatccggagt gggccggcaa
     2521 actgccgcct cccatgtccg acgaacgcga tctgctcacc gagctttcgc actatcgcga
     2581 gaactcgcgg ctgtcctgcc aggtcgaaat caccgcggcc ttgaacgggc tcaaagtcac
     2641 cattgccgaa gacgaataac gcgtagggac ctgcccatgc tgacttccgc cacctggatg
     2701 tacgtgtgcg ccatcatcgg cggcgtcatc gcgggctggc gcatctacca gaacctcaac
     2761 aagctgcgta cgcgcaagaa cgacacctgg gacgagcgcc tcatcgacca gttgcgcaag
     2821 caggggtcgg accccttcaa gccgcacgac gtggatttct tcctggcgtt cccgacgaag
     2881 gaaggcgccg aattcctcgc gtcgcggttc aagaccgaag gtttcgactc ggacgtggtc
     2941 gactcacccg agaacggcga cctgcgttac agcctgcacc tgcacaaatc catgctgctc
     3001 gtggtgccgg acatgaaaga gctgagccgg cgtctgacgg acgcggccaa ggaaaaggaa
     3061 gggcgttacg acggctggag cgccaagccc gtggcgcgcg ccagcgtcga acccgtcta
//
LOCUS       DATDEU010000189         3253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_324055, whole genome shotgun sequence.
ACCESSION   DATDEU010000189 DATDEU010000000
VERSION     DATDEU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324055"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /gene="infB"
                     /locus_tag="VIV63_04590"
     CDS             <1..1627
                     /gene="infB"
                     /locus_tag="VIV63_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY6123907.1"
                     /translation="PIDRDTATLVVEEMGHIAKVLKGDQVEEDLQGEEAPETEAPRPP
                     VVTVMGHVDHGKTSLLDYIRTTKVAAGEAGGITQHIGAYHVETAKGMVTFLDTPGHAA
                     FTAMRARGAKATDVVVLVVAADDGVMPQTIEAIQHAKAAGVPIIVAVNKIDKPDAEPD
                     RVRTELSKHDVIAEEWGGSNMFVQVSAKTGQGVDALLDAILLQAEVLELTAPADGLAT
                     GIVIESSIEKGRGAVATVLVKKGTLKLGDPIIAGNEFGRVRAMFDENGKAVDSAAPSI
                     PVQVLGLQGSPNAGDEFLAVENERKAREVALYRQGKFRDVKLAKQASKMGDVFAQMEE
                     QKLGVIPILLKTDVQGSAEALRDALNKLATAEVQVKIIASGVGGITISDVQLAAASKA
                     RIIGFNVRADAAARDAMKDNEVEVQYFSIIYEAIDYVKALLSGLLAPEIKETIVGTAQ
                     VREVFRSSKFGVVAGCLVTEGTVKRNNPIRVLRDSVVIFEGALESLRRFKDDAGEVRA
                     GTECGIGVKNYQDVRVGDQIECYARVEVARSIQ"
     gene            1656..2036
                     /gene="rbfA"
                     /locus_tag="VIV63_04595"
     CDS             1656..2036
                     /gene="rbfA"
                     /locus_tag="VIV63_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY6123908.1"
                     /translation="MSGKTQRLGRIEAEMQRVLSTLVSREVRDPRVGNITITTVSVAP
                     DMSTARIFFVPFANKHTPEEVTEGLNRASGFLRGALGRALSLRHAPRLEFIYDQQIEN
                     ADKLTRLIDGAVKSDKHDPDPPSE"
     gene            2042..2929
                     /gene="truB"
                     /locus_tag="VIV63_04600"
     CDS             2042..2929
                     /gene="truB"
                     /locus_tag="VIV63_04600"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY6123909.1"
                     /translation="MPNGIILLDKPQGLTSNGALQRVRRAYGAASAGHVGTLDPMATG
                     MLPLCLDEATKVIAEIESGGKCYEFTVQLGARTDTGDAEGQVVEEAAIPPLDATLIER
                     ALAGFRGTIQQVPPMYSALKREGRPLYELARKGIEVERAARTIEIRRLELLAVRPDAL
                     DLVCECAKGTYIRVLGEDIARALGTLGHLTRLRRTWVEPFREMPMLGLEEVLAGAPDA
                     SGLLKPEVALRGLPEAWVTAEQVVALRHGQAVRSAVNPPAPGRRVCLYGPGGVFLGLA
                     EPKPDGWLQPRRLMAQKST"
     gene            3013..>3253
                     /gene="rpsO"
                     /locus_tag="VIV63_04605"
     CDS             3013..>3253
                     /gene="rpsO"
                     /locus_tag="VIV63_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY6123910.1"
                     /translation="MSLATEKKTGVVAQFQRATNDTGSPEVQIALLSERINGLGDHFG
                     AHKRDHASRRGLVKLVNQRRKLLDYLKATKPAKYQE"
BASE COUNT          632 a         1044 c         1060 g          517 t
ORIGIN      
        1 gcccatcgat cgcgacaccg cgacgctggt ggtcgaagag atgggccaca tcgccaaggt
       61 tctgaagggc gaccaggtcg aagaggatct gcagggcgaa gaagcgcccg agaccgaagc
      121 gccgcgtcct cccgtggtca ccgtcatggg ccacgtcgat cacggcaaga cttcgctgct
      181 cgattacatc cgcaccacca aggtggccgc gggtgaagcg ggcggtatca cgcagcacat
      241 cggcgcgtac cacgtggaaa cggcgaaggg catggtcacg ttcctcgaca cgcccggcca
      301 cgcggctttc accgcgatgc gcgcgcgcgg cgccaaggcg accgacgtcg tggtgctcgt
      361 ggtggctgcc gacgacggcg tcatgccgca gaccatcgaa gcgatccagc atgcgaaagc
      421 ggctggcgtt ccgatcatcg tggccgtgaa caagatcgac aagcccgatg ccgaaccgga
      481 tcgtgtccgc accgaacttt cgaagcacga cgtcatcgcc gaagaatggg gtggctcgaa
      541 catgttcgtg caggtgtcgg ccaagacggg tcagggtgtg gatgcgttgc tcgacgccat
      601 cctgctgcag gccgaagtgc tcgagctcac ggctccggcc gacggcctcg ccaccggcat
      661 cgtcatcgaa tcctccatcg agaagggccg tggcgccgtt gccaccgtgc tcgtgaagaa
      721 gggcacgctg aaactcggcg acccgatcat cgcgggcaat gagttcggcc gcgtacgcgc
      781 catgttcgac gagaatggca aggcggtaga tagtgccgcg ccgtccattc ccgtgcaggt
      841 gctgggcctg cagggctcgc ccaacgccgg cgacgaattc ctcgccgtcg aaaacgaacg
      901 caaggcgcgc gaagtcgcgt tgtatcgcca gggcaagttc cgcgacgtga aactcgcgaa
      961 gcaggcctcg aagatgggcg acgtgttcgc gcagatggaa gagcagaagc tcggcgtcat
     1021 cccgatcctg ctcaagaccg acgtgcaggg cagcgccgaa gcgctgcgtg acgcgctcaa
     1081 caagctcgcc accgcggaag ttcaggtgaa gatcatcgcc agtggcgtgg gtggtatcac
     1141 catttccgac gtgcagctgg ccgcggcttc caaggcgcgc atcatcggtt tcaacgttcg
     1201 tgccgacgcg gccgcccgcg acgccatgaa ggacaacgaa gtcgaagttc agtacttcag
     1261 catcatttac gaagccatcg actacgtgaa ggctctgctg tccggcctgc tcgcgcccga
     1321 gatcaaggaa accatcgtcg gcaccgccca ggtgcgcgaa gtgttccgct cgagcaagtt
     1381 cggcgtggtg gcgggctgcc tggtcaccga aggcaccgtc aagcgcaaca acccgatccg
     1441 cgtgctgcgc gacagcgtgg tgatcttcga aggcgcgctg gaatccctgc gccgcttcaa
     1501 ggacgacgcc ggcgaagtgc gtgccggcac cgagtgcggc atcggcgtga agaactatca
     1561 ggacgtccgc gtgggcgatc agatcgagtg ttacgcgcgc gtcgaggttg cccgcagcat
     1621 ccagtgatgc gggcaatcca gatgagcgcc caagaatgag tggaaagaca caaaggttag
     1681 gccgcatcga agcggagatg cagcgcgtcc tgtcgacgct cgtgtcgcgc gaagtgcgtg
     1741 atccgcgcgt cggcaacatc acgatcacca cggtcagcgt cgcgccggac atgtcgaccg
     1801 cgcgcatctt cttcgtgcct tttgccaaca aacacacgcc cgaagaagtc acggaaggtc
     1861 tgaaccgcgc ctcgggtttc ctgcgcggcg cgctcggacg cgcgctgtcg ctgcggcatg
     1921 ctccccggct cgagttcatc tacgaccagc agatcgaaaa cgccgacaag ctcacgcgcc
     1981 tcatcgacgg cgccgtgaaa tcggacaagc acgatcccga tcctccgtcg gaataggatc
     2041 catgccgaac ggcatcatcc tgctcgacaa gccccagggc ctcacgtcga acggcgcgct
     2101 gcagcgcgtg cgacgcgcct atggcgccgc gagcgccggc cacgtcggca cactcgatcc
     2161 gatggccacc ggcatgctgc cgctatgcct cgacgaggcc accaaggtca tcgcggaaat
     2221 cgaatctggc ggcaagtgct acgaattcac ggtgcagctg ggtgcccgga cagatacggg
     2281 tgatgccgag gggcaggtgg tcgaagaggc ggcaattccg ccgctcgacg ccacgctcat
     2341 cgaacgggcg ctcgcgggtt tccgcgggac gatccagcag gtccctccca tgtactccgc
     2401 gctcaagcgc gagggccggc cgctgtacga actcgcgcgc aagggaatcg aagtggagcg
     2461 cgcggcgcga acgatcgaga tccgccgcct ggaattgctg gccgtgcggc cggatgcgct
     2521 ggacctcgtc tgcgaatgtg ccaaggggac ctatatccgc gtgctcggcg aggacatcgc
     2581 acgtgcgctc gggacgctgg gccatctgac ccgcctgagg cggacctggg tggagccttt
     2641 ccgcgaaatg cccatgctcg gtctcgagga agtcctggcc ggcgcaccgg acgccagcgg
     2701 gctgctgaag cccgaggtgg cgttgcgcgg gctgcccgag gcctgggtca cggcggagca
     2761 ggtcgtggcg ctgcggcacg gacaggctgt gcgaagcgcc gtcaatccgc cggctcccgg
     2821 gcgtcgtgtt tgcctgtacg gcccgggagg ggttttcctg ggcctggccg agccgaaacc
     2881 ggacggctgg ttgcagccgc gacggctcat ggctcaaaaa tccacgtaag cctaggcttt
     2941 gcaggacctt ttctgtagta ttcgcgcccg aaaatccagg taataaattc gttctgaagg
     3001 atcaaattta agatgtctct cgccactgaa aagaagaccg gtgtggtcgc ccagttccaa
     3061 cgtgccacca acgacaccgg gtctcccgaa gtccagatcg cgctgctttc cgagcgcatc
     3121 aacggcctcg gcgaccactt cggcgcacac aagcgcgacc atgcttcgcg tcgcgggctc
     3181 gtcaagctcg tgaaccagcg ccgcaagctc ctcgactatc tcaaggccac caagcctgcg
     3241 aagtaccagg agg
//
LOCUS       DATDEU010000190         7014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_178507, whole genome shotgun sequence.
ACCESSION   DATDEU010000190 DATDEU010000000
VERSION     DATDEU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178507"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1038)
                     /locus_tag="VIV63_04610"
     CDS             complement(292..1038)
                     /locus_tag="VIV63_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6123911.1"
                     /translation="MDYAVVGNALQVVSEELCETVNLCQDQRVLDVATGNFNAALAAA
                     RRWCDVTATDHASDLLDRRRQRLEAESTGVRIVDGDVDGLPFGDQSFDAVLSCFGAMY
                     AADQERAASEMIRVCRRGGQVGLANWTPDGFIGQLFRMVSRHAPSGAAAMTCDWGTQG
                     RIDELFGAYGNVEAVARRIAFRCRTPMDWVDKLRAVYLPVHGIFATLDADRKRALRSE
                     LLELVAKFNRATDGSMVVDAAYLEVTITRR"
     gene            1180..3186
                     /locus_tag="VIV63_04615"
     CDS             1180..3186
                     /locus_tag="VIV63_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123912.1"
                     /translation="MGTSVESLEFRLLGPLQLSRGGEVLKLPSSRKVRALLGYLVLAS
                     RPVARSQVCELLWDVPNDPRGELRWCLSKIRGLIDEKGRKRVIADGSTIRLDLTDCVV
                     DTLEVTRAPEHGIQKLGVDRQQALAAHFAGELLEGLEIARSPMFDAWITAERRRFRGI
                     QTVLLENLARSLPHETAASYIDEWLKLSPFDRNAHELLLGSLARQGRIADGEAHLASA
                     IKLFEADGLDGAPLRDIWRRTREKHAAPAAPKPALEIISTSLTEIRQVSPSTPAATRR
                     ASIAVMPFVDRSTEADKRGGAADALAYDMITRLAKLRSMFVIAQGTTFALRDRAIGPE
                     EAGRMLNVDYVVSGSVRRAGRKFTVSADLIETRTARIVWAEVFDEKVDDTLDVLDEIG
                     NRIVASVAHEIEMVERNRAILKPPNSLDAWEAHHRGLWHMYRFNKTDNERARHFFAQA
                     VDLDPTFARAYAGLSFAHFQNAFLGWKKPAPEIDRAFDAAGKSLMVDDRDPAAHWAMG
                     RAMWLRGGQDQAVMELERAVDLSPNYATGHYTLAFVHSQTGDPHAAISYSDHSRHLSP
                     FDPLLFAMLGTRAVSLARLGRYEEAAEWAMKAAARPNAHHHIMAIAAFTLGLAGRVPD
                     AAAYKARILERVPNYAMTDFLAAFRYTPDAEALFKQAGKKVGIS"
     gene            3197..3835
                     /locus_tag="VIV63_04620"
     CDS             3197..3835
                     /locus_tag="VIV63_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017398157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY6123913.1"
                     /translation="MMPAHELMLFALAALIMVLTPGPNMIYLVSRSLCQGRKAAVISL
                     FGVAAGFLVHMFAAAAGLTAMFMAIPLAYEILKWLGAAYLLWLAWQAVRPGAGSSPFD
                     PRDLPADSPARLFVMGFLTNALNPKIAVFYLAVFPQFIRPEHGAVLGQSVQLGLIQIA
                     ISFAVNLALILTAAHLAAWFARNPLWLAAQRWVMGFVLAGLAVRLALDSRRN"
     gene            3881..4225
                     /locus_tag="VIV63_04625"
     CDS             3881..4225
                     /locus_tag="VIV63_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123914.1"
                     /translation="MKTLERLFVIALLGFAPLAVAQINMPNPNQPGASMESAVRVVVT
                     SDLMVDRFIERWLRTHYPGWDADPHEFMEVGPERFAVVYITSPNNPGRRVYFRVQKHQ
                     NEDDGMGMPPPL"
     gene            4329..4508
                     /locus_tag="VIV63_04630"
     CDS             4329..4508
                     /locus_tag="VIV63_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY6123915.1"
                     /translation="MSTENPYAQYHVVKKGDTLSKIAEEYYGDKMLYPKIFEANKDVL
                     TDPNKIKPGQKLRIP"
     gene            4526..5128
                     /locus_tag="VIV63_04635"
     CDS             4526..5128
                     /locus_tag="VIV63_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123916.1"
                     /translation="MRIANLFVALAATTLMLAGCASQKEPAEKALAQVESSLAEFRAD
                     AEKYATEELQAVDNSIAKMKASVANKDYSAVVKAAPALNSTISSLKQTVATKKADAEA
                     MLAAAQAEWTDLSTKVPQMVEAIQSRVDTLGKSRKLPANVDKATWETVKTNFETMKTE
                     WTDATAQFAAGEAGEAVRKAREVKSKGDEILRQLGMTPNA"
     gene            complement(5125..6234)
                     /locus_tag="VIV63_04640"
     CDS             complement(5125..6234)
                     /locus_tag="VIV63_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123917.1"
                     /translation="MNKPALFAALLLGVATGAPAADLVVHEWGTITTIHAADGTPKGG
                     LNSIDEADVLPDFVHRYEPETTRQKPGLILGKLPNIPGRPDVTMRLETPVIYFHPPPN
                     QKFTLPIDISVRFRGGVINEFYPDAEASVALDTERVLSKRRAGVIKQWDGDVLNNYVV
                     GSLDWKGLRLHDTVVAPLTKSEIWLAPREVSSVAVFNPTAGEGDQYLFYRGVAHLDAL
                     LSTKTSRNSVALATPANLTWLESGAAVLPNVWLAEVRADGVIAFRAHPAVTLKTENAG
                     KALAQIKRFSEGDFTAAGAPQLRSSLKKALIAQGLFADEAEAMLNTWKISYFQKPGLR
                     VFYIVPREWTDYFLPLEFSVPARVTRVIVGRIDLN"
     gene            complement(6293..>7014)
                     /locus_tag="VIV63_04645"
     CDS             complement(6293..>7014)
                     /locus_tag="VIV63_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123918.1"
                     /translation="TRLLRSQAETALGELVSAGLVNADSYSGLRALLIPSDKKRQLVA
                     RRRRVALFGLEDAGRWSLIRKGATRNDAESLEQVAEILLRRYGVVFRKLLEREADWLP
                     PWHSLLRVFRRLEAQGKIRGGRFVAGISGEQYALPDAVSSMRAIRRQEAKGDFVSLSA
                     ADPLNLTGIITPGARVPALARNRVLYRDGVPVATHAGGQSEFIVALPPEKEWEARQAL
                     VRRNIQPGARAAAARGERSAH"
BASE COUNT         1290 a         2326 c         2202 g         1196 t
ORIGIN      
        1 ggagaacgga agatttccaa cgtaaagttt catgaatgaa ttccaatgga cagtggacct
       61 acggaaaatc ccgtcggtcg gttcgcgaat gctttgaggt gactgactct ggccggtaat
      121 gcgtcaggcg acgcgggtcc agggacggac aagacgaagg atcacaagcc gggtctcaag
      181 gtactccaaa tgcgccaacc gcatgggtct aaatgggcgc tgcggcgcca aatgcgcccg
      241 gcagagcccg cagatccctg tttttgcgcg gtttccgggc aggcgctcgc gtcagcgccg
      301 agtgatcgtg acctcgagat aggccgcgtc cacgaccatg cttccgtcgg tcgcgcgatt
      361 gaatttcgcg acaagctcta acagctccga gcgcagcgcg cgtttgcgat ccgcgtcgag
      421 cgtcgcgaag atgccgtgga cgggaagata gacggcgcgc agtttgtcga cccagtccat
      481 gggggtgcgg cagcgaaacg cgatgcgcct ggcgaccgct tccacattgc cgtaggcgcc
      541 aaacaattcg tcgatgcggc cctgcgttcc ccagtcgcag gtcatggcgg ccgctcccga
      601 tggcgcatgg cggctgacca tcctgaacag ctggccgatg aacccgtccg gcgtccagtt
      661 ggcgagaccc acctgtccac cgcgccggca cacacggatc atttcggacg cggcgcgctc
      721 ctggtcggcg gcatacatgg ccccaaagca ggacaacacc gcgtcgaagc tctggtcgcc
      781 gaagggcagc ccgtcgacat cgccgtcaac gatgcggacg cccgtggatt cggcctcgag
      841 gcgctgacgg cgccgatcca gcagatccga agcgtgatcc gtcgcggtga cgtcgcacca
      901 gcggcgggcg gcggcgaggg cggcgttgaa attgcccgtg gccacgtcga gcacgcgctg
      961 gtcctggcac aaattcacgg tttcgcagag ttcctcgctg accacctgca gtgcattgcc
     1021 gacgacggca taatccatgc tcgcgtacgg cagctgccgg cgcgcaacga ctgcccgatg
     1081 atcgattcgc ggagcgtttc tcgaaagaac tgcgttcatg acatccctct tccatccctg
     1141 ttcctccttc aaatctacgg atctcgcgtg gtagcgggcg tgggaacgtc cgtggaatcg
     1201 ctcgaattcc gcctgctggg tccgctgcag ctttcgcgcg gcggcgaggt cctgaagctc
     1261 ccgtcatcgc gcaaggtacg cgccctgctc ggctacctcg tgctggccag caggccggtg
     1321 gcgcgctcgc aggtgtgcga actcctgtgg gacgtgccta acgacccgcg cggcgagctg
     1381 cgctggtgtc tctcgaaaat tcgcgggctc atcgacgaga aaggccgcaa acgcgtcatc
     1441 gccgatggca gcacgatccg gctcgacctc accgactgcg tggtcgacac gctcgaagtc
     1501 acgcgcgcgc ccgaacacgg aatccagaag ttaggcgtcg accggcaaca ggcgctggcc
     1561 gcgcatttcg ccggcgaact gctcgaaggg ctggaaattg cccgaagccc catgttcgac
     1621 gcgtggatca ccgccgagcg ccggcgattc cggggcatcc agaccgtgct gctcgagaac
     1681 ctcgcgcgtt cgctgccgca cgagaccgct gcgtcctata tcgacgagtg gctcaaactc
     1741 tcgccgttcg accgcaatgc gcacgaactg ctgctcgggt cgctcgcccg gcagggacgc
     1801 atcgccgacg gcgaggctca cctggcgtcg gccatcaaat tattcgaagc cgacgggctc
     1861 gacggcgcgc cgctgcgcga catctggcgg cgcacgcgtg aaaaacatgc ggccccggct
     1921 gcgccaaagc cggcgctgga aatcatcagc accagcctca cggagatccg ccaggtctca
     1981 ccgagcacac ccgcggcgac gagacgcgct tcgatcgcgg tcatgccgtt cgtcgatcgc
     2041 tcgacggaag ccgacaagcg tggcggcgcg gccgacgcgc tggcgtacga catgatcacg
     2101 cggctcgcga aactgcgcag catgttcgtg atcgcccagg gcaccacgtt cgcgctgcgc
     2161 gatcgcgcca tcgggcccga agaagccggc cgcatgctca acgtcgacta cgtggttagc
     2221 ggctcggtgc gccgcgccgg gcgcaagttc accgtgagcg ccgatctcat cgagacgcgc
     2281 accgcgcgca tcgtctgggc agaagttttc gacgagaaag tcgacgacac actcgatgtg
     2341 ctcgacgaaa tcggcaaccg catcgtcgcc tccgtggcgc acgagatcga gatggtcgag
     2401 cgcaatcgcg ccatcctcaa gccgcccaat tcgctcgacg cgtgggaagc gcatcaccgc
     2461 ggcctctggc acatgtaccg gttcaacaag accgacaacg agcgggcaag gcatttcttc
     2521 gcgcaggccg tcgatctcga ccccacgttt gcacgcgcct acgccggact gtcgttcgcg
     2581 catttccaga acgcgttcct cggctggaag aagcccgcgc cggaaatcga tcgcgcgttc
     2641 gatgccgccg gcaagagcct catggtcgac gaccgcgatc cggccgcgca ctgggccatg
     2701 ggccgcgcca tgtggctgcg cggcggacag gaccaggcgg tcatggaact cgagcgcgcc
     2761 gtcgatctct cgccgaatta cgccaccggt cactacacgc tggcgttcgt ccattcgcag
     2821 acgggcgatc cgcatgcggc catctcatac tcggatcatt cgcggcatct gtcgcccttc
     2881 gatccgctgt tgttcgcgat gctcggcacg cgtgccgtca gcctggcccg gctcggccgc
     2941 tacgaggaag ccgccgaatg ggccatgaag gccgcggcgc gtcccaacgc gcatcaccac
     3001 atcatggcca tcgccgcgtt cacgctgggc ctcgcgggac gcgtgccgga cgccgcggcc
     3061 tacaaggcgc gaatcctcga gcgtgtaccc aactatgcga tgacggactt cctggcggcg
     3121 tttcgttaca cgccggacgc cgaggcgctg ttcaaacaag ccggcaagaa ggtcggcatt
     3181 tcatgaggcc ctcacgatga tgcctgccca cgagttgatg ctgtttgcgc tcgccgcgct
     3241 catcatggtg ctcacgccag ggccgaacat gatctacctc gtgtcgcgtt cgctgtgcca
     3301 gggcagaaag gcagcggtca tctcgctgtt cggcgtggcg gccggattcc tcgtgcacat
     3361 gttcgccgct gccgcgggac tcaccgccat gttcatggcc attcccctcg cctacgagat
     3421 cctcaagtgg ttaggcgctg cgtacctgct gtggctcgcg tggcaggccg tcaggcccgg
     3481 cgccgggtcg tcgccgttcg atccccgtga cctgcccgcc gactcgccag cccggctttt
     3541 cgtcatggga ttcctgacca atgcgctgaa tcccaagatc gcggtgttct acctcgcggt
     3601 gttcccccag ttcattcgcc cggaacacgg ggcggtgctc gggcagagtg tccagctcgg
     3661 gctcatccag atcgccatca gttttgccgt aaacctggct ttgatcctca cggccgccca
     3721 tctggcggcc tggttcgcgc gaaatcccct gtggctcgcc gcacaacgct gggtgatggg
     3781 tttcgtgctg gcgggtctcg cggtacggct ggctctggat tcaagacgca attgacgatc
     3841 ctcggatggc tatgatttat tggccaggca agggggcccc atgaaaacgc tcgagcgact
     3901 cttcgtcatc gcattgttag gtttcgcgcc gctggccgtc gcgcagatca acatgccgaa
     3961 tccaaaccag cccggcgcct cgatggaaag cgcggtccgc gtcgtcgtca ccagcgacct
     4021 gatggtggac cgcttcatcg agcgatggct gcgcacccac taccccggct gggatgccga
     4081 tccgcacgaa ttcatggaag tgggtccgga acggttcgcg gtggtctaca tcacctcacc
     4141 gaacaaccct ggacgccgcg tctacttccg tgtgcagaag caccagaacg aagatgacgg
     4201 catgggtatg ccgccgccgc tctgagcgct tgctgcgttg cgattgacgt cagagcaatc
     4261 tgatagctag tccgggtacg ccggtccata ctcgccgcgc cctggctcaa cgactcgagg
     4321 tcgccagaat gtccaccgaa aatccttacg cgcaatatca cgtcgtgaag aagggtgaca
     4381 cgctctcgaa gatcgccgag gaatattacg gcgacaagat gctctacccg aagatcttcg
     4441 aggcgaacaa ggacgtcctg acggacccta acaagatcaa acccggccag aagctgcgga
     4501 ttccataatt tttgaggaga ccctgatgcg tatcgcaaac ctgttcgtgg cgcttgccgc
     4561 caccacactg atgctggccg gctgcgccag ccagaaggaa cccgccgaga aggcgctggc
     4621 ccaggtcgaa tcctcgctcg cggaattccg cgccgatgcg gagaaatacg ccaccgaaga
     4681 gttgcaggcc gtcgacaatt cgatcgccaa gatgaaggcc agcgtggcca acaaggatta
     4741 cagcgccgtc gtgaaggccg cgccggcgtt gaactcgacc atctcctcgc tcaaacagac
     4801 ggtcgccacc aagaaggcgg acgcggaagc catgctggcc gcggcgcagg cggaatggac
     4861 cgacctctcg accaaggttc cgcagatggt cgaagccatt cagagtcgcg tcgacacact
     4921 gggcaagtcc cgcaagctgc ccgccaacgt ggacaaggcg acgtgggaga cggtcaagac
     4981 gaacttcgag accatgaaga ccgaatggac cgacgccacc gcgcagttcg ctgccggaga
     5041 agcgggcgaa gcggtgcgca aggcgcgcga agtgaagtcc aaaggcgacg agatcctgcg
     5101 tcagctcggc atgacgccga acgcctaatt gaggtcgatg cgaccgacga tcacgcgcgt
     5161 gacgcgcgcc ggcacggaaa attcgagcgg caggaaatag tcggtccact cgcgcggcac
     5221 gatgtagaac acgcggagcc ccggcttctg gaagtagctg atcttccagg tgttcagcat
     5281 cgcctccgct tcgtcggcga aaaggccctg ggcgatcagg gcctttttca atgaacttcg
     5341 aagttgcggc gcgccggcag ccgtgaagtc cccctcggaa aagcgcttga tctgcgccag
     5401 cgccttgcca gcgttctctg tcttcagtgt cacggccgga tgtgcgcgga acgcgatgac
     5461 accgtcggcg cgaacctcgg cgagccagac gttcggcagg actgccgcgc cggattcgag
     5521 ccacgtgagg ttggccggcg tcgcgagcgc aaccgaattc cgggaggtct tcgtcgacag
     5581 cagcgcatcg agatgcgcga ctccgcgata gaaaagatat tggtcgcctt ccccggccgt
     5641 gggattgaac accgcaaccg aactcacttc gcgcggcgcg agccagattt cactcttcgt
     5701 cagcggcgcc accaccgtgt cgtgcaggcg cagccccttc cagtcgagcg accccaccac
     5761 gtagttgttg agcacgtcgc catcccactg cttgatgacg cccgcccggc gtttcgacaa
     5821 caccctttcg gtgtcgagcg cgaccgacgc ttccgcgtcg ggataaaact cgttgatcac
     5881 accgccgcga aaccgcaccg agatgtcgat gggcagcgtg aacttctgat tcggtggcgg
     5941 gtgaaaatag atgaccggtg tttcaagccg catcgtcacg tccggccggc ccgggatgtt
     6001 gggcagcttg ccgaggatca ggcccggctt ctggcgcgtg gtttccggtt catagcgatg
     6061 cacgaaatcc ggcaggacat cggcttcgtc gatgctgttg agaccgccct tgggtgtgcc
     6121 gtcggcggca tggatggtgg tgatcgtgcc ccactcgtgc accacgagat cggcggcggg
     6181 ggcgccggtg gcgacaccca gcagcaaggc agcaaacaaa gcgggcttgt tcatcgcgcg
     6241 aatctccggc ggccatgtcg gaagactacc gcgcgcgaac aaggttcgct cttcagtgag
     6301 ctgaacgctc gccgcgcgct gctgcggcgc gcgccccggg ctggatattc ctgcgcacca
     6361 gcgcctggcg cgcctcccat tctttttccg gcggcagcgc gacgatgaat tccgactgcc
     6421 cgcccgcgtg cgtcgccacc ggaacgccgt cgcgatagag cacgcgattg cgcgccagcg
     6481 ccggcacccg tgcgcccggc gtgatgatgc cggtgagatt cagcgggtcc gccgcgctca
     6541 gcgatacgaa gtcgcccttc gcttcctgcc ggcggatcgc gcgcatggac gacaccgcat
     6601 cgggcaacgc gtactgctcg ccgctgatgc ccgcgacgaa acgtccgccg cgaatcttgc
     6661 cctgcgcttc gagccggcgg aacacgcgca acagcgaatg ccacggcggc agccagtcgg
     6721 cctcgcgttc cagcagcttg cggaatacga caccgtatcg gcgcaagagg atctcggcca
     6781 cctgctccag cgactccgcg tcgttacgcg tggcaccctt gcggatgaga ctccagcgac
     6841 ccgcgtcttc gagaccgaac aacgccacgc gccggcggcg cgccacgagc tggcgcttct
     6901 tgtcgctcgg gatcagcagc gcgcgcaggc ccgaatagct gtcggcgttg accaggcccg
     6961 cggataccag ttcgccgagc gcggtttccg cctgcgatcg cagcagacgt gtgc
//
LOCUS       DATDEU010000191         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1346258, whole genome shotgun sequence.
ACCESSION   DATDEU010000191 DATDEU010000000
VERSION     DATDEU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346258"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VIV63_04650"
     CDS             complement(<1..122)
                     /locus_tag="VIV63_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY6123919.1"
                     /translation="MKFVDEARVKVQAGNGGRGCVSFRREKFVPFGGPDGGDGG"
     gene            complement(195..452)
                     /gene="rpmA"
                     /locus_tag="VIV63_04655"
     CDS             complement(195..452)
                     /gene="rpmA"
                     /locus_tag="VIV63_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY6123920.1"
                     /translation="MAHKKAGGSTRNGRESHSKRLGVKKFGGEAVLAGNILVRQRGTT
                     MRAGTNVGIGTDHTLFALKHGRVKFSKKGAEQHTFVSVDAE"
     gene            complement(491..802)
                     /gene="rplU"
                     /locus_tag="VIV63_04660"
     CDS             complement(491..802)
                     /gene="rplU"
                     /locus_tag="VIV63_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY6123921.1"
                     /translation="MYAVIATGGKQYRVENGTILKVEKLDAEPGATIEFGEVLLIGNG
                     DDIKIGAPLLKGGKVTATVEKHGKGEKKVIVKFRRRKHYLRQGTHRQQFTQVKITGIS
                     A"
     gene            1010..1978
                     /gene="ispB"
                     /locus_tag="VIV63_04665"
     CDS             1010..1978
                     /gene="ispB"
                     /locus_tag="VIV63_04665"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF008140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEY6123922.1"
                     /translation="MPLEEIRALVGADLTAVDAMIRARLKSAVPLVDQVAEHIIGGGG
                     KRLRPLLCVLSGRACGIETDKHIEAAAFIEFVHTATLLHDDVVDGSQKRRGRATANNI
                     FGNQASVLVGDFVYSRAFQMMATVGSQRVMEIMSDATNVIAEGEVLQLMNAHDPETTE
                     QRYLEVIYRKTGRLFEAGAEVAAVLAGAPAAQQAALARYGKHLGTAYQLVDDVLDYRS
                     DPATRGKNLGDDLAEGKPTLPLLHALRHGNDEQRALIRLAIEQGGLAQLGPIVEAIEA
                     TGGLEYAAGFAQRETAQALAALEALPDTPYSRALASLARFAQERTS"
     gene            complement(1999..>2455)
                     /locus_tag="VIV63_04670"
     CDS             complement(1999..>2455)
                     /locus_tag="VIV63_04670"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEY6123923.1"
                     /translation="GLVLDGLATLLIESPLLPKWEHRYFMGEALHLLAVPVLAAGYVS
                     AVVVAFQSGLGRRLLMPFGAVGRMALTNYLTQSLVIGFVLFGIGPGLALAGKIGTLAI
                     AGIVIAFFAVQVIVSRWWLGRYAYGPMEWVWRALTYGERPPMRRSGPVC"
BASE COUNT          459 a          829 c          732 g          435 t
ORIGIN      
        1 tggcctccat cgccgccatc cgggcctccg aaaggcacga acttctcgcg acggaagctc
       61 acgcagccgc gaccgccgtt accggcctga acttttacgc gtgcttcgtc gacgaatttc
      121 atgatgtatt tcctgcgggt acaaaaaacc cggcctgacg ccgggttttt cgatcgcgaa
      181 cagcgacggc gcgattactc ggcgtcgacg ctcacgaagg tgtgctgctc ggcgcccttc
      241 ttggagaact tgacgcggcc gtgcttgagg gcgaacagcg tgtgatcggt gccgatgccg
      301 acgttcgtgc ccgcgcgcat cgtggtgccg cgctggcgga ccaggatgtt gccggcgagt
      361 acggcttcac cgccgaactt cttcacgccc aagcgtttgc tgtgcgattc gcggccgtta
      421 cgagtactgc cgcctgcctt tttatgtgcc atgtgagtcg ggctccaact aactaatagg
      481 acagcagcgc ttaggcgctg atgccggtga tcttgacctg cgtgaactgc tggcgatgcg
      541 tgccctggcg caggtagtgc ttgcggcgac ggaacttcac gatgaccttc ttctcgccct
      601 tgccgtgttt ttcaacggtg gccgtgacct tgccgccctt cagaagagga gcgccgatct
      661 tgatgtcgtc gccgttgccg atgagcagga cttcgccgaa ctcgatcgtt gcgcctggct
      721 ctgcgtcgag cttctccacc ttgaggatgg tgccgttctc gacccgatac tgtttaccgc
      781 cagtggcgat gaccgcgtac atcttgataa ctccggaaac ccgttcgaaa ccaaagcctt
      841 aagccctgtg ccatttcgaa gggaggcgca ttctacgtat cgcccccggg ccggtcaaac
      901 acccggtaaa cagataaata atcccctaga atcaacgccc taaatgagct ccaaagtaac
      961 atcgctgccc acccagcgcg ccgcaccccg ccccaccgac ccgcctgcca tgcccctcga
     1021 ggagattcgc gccctggttg gcgccgacct taccgcggtg gacgccatga tccgggcgcg
     1081 cctgaagagc gcggttccgc tcgtggacca ggtcgccgaa cacatcatcg gcggcggcgg
     1141 gaaacgactg cgccctcttc tatgtgtgct gtcgggccgc gcctgcggca tcgaaaccga
     1201 taaacacatc gaggccgcgg cgttcatcga gttcgtccat accgccacgc tgctccacga
     1261 cgacgtggtg gatggctcgc agaagcggcg cggccgcgcc acggctaaca acattttcgg
     1321 caaccaggcg agcgtgctgg tcggggactt cgtctactca cgcgcgttcc agatgatggc
     1381 caccgtcggt tcgcagcggg tcatggagat catgtcggac gccacgaacg tgatcgccga
     1441 aggcgaagtg ctgcagctca tgaacgcgca cgacccggag accaccgagc agcgttatct
     1501 cgaagtgatc taccgcaaga ctggccgctt gttcgaggcg ggcgcggaag ttgccgcggt
     1561 tctcgccggc gctccggctg cccagcaggc ggcactcgcg cgttacggca agcatctcgg
     1621 taccgcctat caactcgtcg acgacgtgct cgactaccgg tcggatccgg ccacgcgtgg
     1681 caagaatctg ggcgacgatc tcgccgaggg caaacccacg cttccgttgt tgcatgcctt
     1741 gcgacacggc aacgacgagc agcgcgcgct cattcgcctc gccatcgaac agggcggact
     1801 cgcgcagctc ggcccgatcg tcgaagccat cgaagcgacc ggtggcctgg aatacgccgc
     1861 cggattcgcg cagcgcgaaa ccgcgcaggc gctcgcggcg ctcgaggcct tgccggatac
     1921 gccgtattcg cgcgcgctgg cttcactggc tcgcttcgcg caggaacgca cctcgtaaat
     1981 ggggtcagac cccatttgct agcaaacggg gccggacctg cgcatgggcg gccgctcgcc
     2041 ataggtcaat gcgcgccaca cccattccat cggcccgtag gcataccggc ctaaccacca
     2101 gcgactgacg atcacctgaa cggcaaagaa cgcaatcacg atgccggcga ttgccagcgt
     2161 gcctatcttc cccgccagcg ccagccccgg accgatgccg aacagcacga aaccgatcac
     2221 caggctctgg gtcagatagt tagtcagcgc catgcgcccg acggctccga acggcatcaa
     2281 cagcctgcgc ccgagtccgc tctggaacgc gacgacgacg gccgacacat agccggcggc
     2341 gagcacgggc accgccaaca ggtgcagcgc ttcccccatg aagtaccggt gctcccactt
     2401 cggcagcaag ggtgattcga tgagcagcgt cgccaaacca tcgagcacga ggccc
//
LOCUS       DATDEU010000192         5875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1929292, whole genome shotgun sequence.
ACCESSION   DATDEU010000192 DATDEU010000000
VERSION     DATDEU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929292"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VIV63_04675"
     CDS             complement(<1..254)
                     /locus_tag="VIV63_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123924.1"
                     /translation="MVAFIKFTRGRIIFIAAVIALFAAYTLFGFFGVPRLLHSNATGF
                     VAKEYGRKLDLGEIEFNPFTLVLEVHGLSFPDTEARPLVG"
     gene            367..579
                     /locus_tag="VIV63_04680"
     CDS             367..579
                     /locus_tag="VIV63_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123925.1"
                     /translation="MARGSTTVFVECRRCGHKGQREVAFLPLPAGARWRCSMCRNREP
                     GTIRTWHTGGSSSARVISFPKGNRRS"
     gene            626..2044
                     /locus_tag="VIV63_04685"
     CDS             626..2044
                     /locus_tag="VIV63_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6123926.1"
                     /translation="MGRHAVSRWAWLAWTLLAGTCVAAEPNEIWATDLDDLRERRVVR
                     ILVAYGKTFYFVDSGQPHGISYELGAALEKDLNRNNPDRARPVRVMFIPVRRDRMLPS
                     LIEGRGDIAAVNLTITPERLELVDFSAPFAEGVSEIFVTSNKGGAPLSVYDLSGRSVF
                     VRRSSSYFASLEELNRVLRAKGKPPAKIVLADENLEDEDILEMLNADLIDATVVDSYV
                     ASFWSQLLPNIRAHPQAAVRGAAQIAWAIRKDSPLLKAELATFVRNNRVGTTTGNILL
                     RRYLRDTRWAKNATAESDMRRFSELSKYFQKYATQYRFEWLMLVAQGYQESGLDQNAR
                     SPVGAIGVMQVMPTTAGDRAVNIPDIHLVDQNIHAGVKYLRYLVDQFFNEPGIDLLNR
                     HLFAFAAYNAGPTRIAKLRREAAAAGLDPNTWFYNVELIAAREIGRETVQYVSNVFKY
                     YIAYKLVVERARERAAIKRQAG"
     gene            2131..3411
                     /locus_tag="VIV63_04690"
     CDS             2131..3411
                     /locus_tag="VIV63_04690"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY6123927.1"
                     /translation="MFKPTFTALGLVACAFIQPAQAGPSQYRENDPAVITEWNSIAES
                     VIPASAGVTLPRTYAMMHIAMFDAVNSIDGGYTAYHVRIPAWDGASSEAAAAQAAHDV
                     LTALLPAGTATYDAALAARLAKIHSGRAYHGASVGREVAKKILEWRSTDGWSTPQTFT
                     PPALPGVWQPTPPAFAAAAFVQAGDARPFALPTPYYYLPRRPPSLNSQEYADAVNEIK
                     AIGGATSTVRTAEQTLQARLWASVGYKDLWSSVWNQVTRFMATNRNLSIGESARLFAL
                     VNASMQDGVQTAQASKFVFQLWRPVTAIQRAGEDLNPMTDADPTWMPLLTTPPYPSYA
                     GNMACIGASTARALALYFGTNDLPFTVQWTGLNGNADVARPFPGFWQLAEHQGASREY
                     GGIHYHFDTTASQETCPKVAGYVFANYMRPKKDW"
     gene            complement(3500..3880)
                     /locus_tag="VIV63_04695"
     CDS             complement(3500..3880)
                     /locus_tag="VIV63_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123928.1"
                     /translation="MNADLERQPNPVEQARNLLRVVFKHEQARDPRGSDAGTLERMLG
                     HLHNIEKSVTASMEDHVNASKRLRDFAETLERMKERLSSAPHQEQIPQINALVASLRK
                     ASTELGASGAPPAWASSTPRRPPT"
     gene            4065..4406
                     /locus_tag="VIV63_04700"
     CDS             4065..4406
                     /locus_tag="VIV63_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6123929.1"
                     /translation="MKTPEVIGALGALAHEYRLAIYRLLVEQGPAGLPAGVIGERVGL
                     VPSSLTFHLKSLQSAGLVTQLRASRQLIYSADFAVMNALVGYLTDKCCSAGESCAVEC
                     KPARPRRRNAA"
     gene            4403..4831
                     /gene="arsN2"
                     /locus_tag="VIV63_04705"
     CDS             4403..4831
                     /gene="arsN2"
                     /locus_tag="VIV63_04705"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HEY6123930.1"
                     /translation="MSIISARPSLAAVQSLLESAQLPTADLTADHCEHFFYLGSPATP
                     AGLVGLELFGDVALLRSLAVTPADRASGNGSALLEHAETYARSAGVKFLYLLTTTAED
                     FFARRGYTRTARDAAPPAISTTREFAGICPASSAFMSKPL"
     gene            4845..5357
                     /locus_tag="VIV63_04710"
     CDS             4845..5357
                     /locus_tag="VIV63_04710"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY6123931.1"
                     /translation="MSEQPFNVLILCTGNSARSILGEALINHWGRGKFIGYSAGSTPK
                     GQVHPIALDLLRQLKMPTDGLRSKSWNEFAAPGAPRLDFVFTVCDNAAGESCPYWPGQ
                     PMTAHWGVEDPAAVEGTDTEKWLAFRTAFRELENRIKVFTALPIRTLDRAKLQAHLHE
                     IGGRSLKEVG"
     gene            5357..>5875
                     /locus_tag="VIV63_04715"
     CDS             5357..>5875
                     /locus_tag="VIV63_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY6123932.1"
                     /translation="MQRAVAAEFLGALLLTAAVVGSGIMGEQLAGGNAAVALLANTGA
                     TVAALATLIAVFGPVSGAHFNPVVTLVAFHQRQLTLPRTVAFIGAQVAGCCAGAMLAN
                     VMFELPAIAASTHVRTGSSQWLSEVVATAGLVYIAFSSPASAAAWRVPAWIGAAYWFT
                     ASTSFANPAITLG"
BASE COUNT         1039 a         1983 c         1801 g         1052 t
ORIGIN      
        1 aaacccacga gcggccgcgc ttcggtgtcg ggaaaggaca aaccatgcac ctcgagcacc
       61 agcgtgaacg ggttgaactc gatttcgccg agatcgagct tgcggccata ttccttcgcc
      121 acgaacccgg ttgcgttcga atgcagcagg cgcggcacgc cgaaaaagcc gaacagcgtg
      181 tacgcggcga acaacgcgat gacggcggcg atgaagatga tccgtccgcg cgtgaacttg
      241 atgaacgcca ccatgattcg gggattacac ctttctttat gtgactgtac aggggcctaa
      301 cggcctatac aggccggaag cctcgcgctt gctgtggaca ttgtgtcgca aagtacgctt
      361 cgaggcatgg ccaggggatc caccaccgtc ttcgtcgaat gccgccgctg cggccacaag
      421 gggcagcgcg aagtagcgtt cctgcccctg ccggccggcg cgcgctggcg ctgctccatg
      481 tgccggaacc gcgagcccgg aaccatcagg acctggcata cgggcgggtc gtcgtcggcg
      541 cgcgtcatct cgttcccgaa gggcaaccgc cgtagttaga tcgtcgtgct ggcgatcggc
      601 cggccagcag gagtagtctc gcgccatggg acgacacgcg gtatcgcgat gggcctggct
      661 ggcgtggacg ctgttagccg gcacctgcgt cgccgccgaa cccaacgaga tctgggccac
      721 cgatctcgac gacctcaggg agcgccgcgt ggtgcgcatc ctcgtggcgt acggcaagac
      781 tttctatttc gtcgacagtg gtcagcccca cggcatttcc tacgagctcg gcgccgcgct
      841 cgagaaagac ctgaaccgca acaacccgga ccgcgcgcgg ccggttcgcg tgatgttcat
      901 tcccgtccgg cgcgaccgca tgctgccgtc gctcatcgaa ggccgcggcg acatcgccgc
      961 cgtgaatctc acgatcacgc ccgagcgcct ggagctcgtg gatttcagcg cgccgttcgc
     1021 cgaaggcgtc agcgagatat tcgtcacgtc caacaaaggt ggcgccccgc tgtccgtcta
     1081 cgatctttcg gggcgcagcg tgttcgtgcg ccgttcgagc agctacttcg ccagcctcga
     1141 ggaactgaac cgagtgctca gggcaaaggg caaaccaccg gcgaagatcg tgctggccga
     1201 cgagaacctc gaagacgaag acattctcga gatgctgaac gccgacctca tcgacgccac
     1261 cgtggtcgat tcatacgtcg cgagcttctg gtcgcagctc ctgcccaaca tccgcgccca
     1321 tccccaggcg gcggtgcgag gcgccgcgca gatcgcctgg gccatccgca aggacagtcc
     1381 gctactgaaa gccgaactcg ccacgttcgt caggaacaat cgcgtcggca ccaccaccgg
     1441 caacatcctg ttgcgccgct atctgcgaga tacacgctgg gcgaagaacg ccaccgccga
     1501 gtccgacatg cgccgcttca gcgagttgtc gaagtacttc cagaagtacg ccacgcaata
     1561 ccgtttcgaa tggctgatgc tggtggcgca gggttaccag gaatccgggc tcgatcagaa
     1621 cgcgcgaagt cccgtgggcg cgatcggcgt catgcaggtc atgcccacga ccgccggcga
     1681 ccgtgccgtc aatattcccg acatccacct cgtggaccag aacatccatg ccggcgtgaa
     1741 gtacctgcga tacctggtcg accagttctt caacgagccc ggcatcgacc tgctgaatcg
     1801 ccacctgttc gccttcgccg cctacaacgc gggccccacc cgcatcgcga aattgcgccg
     1861 cgaggccgcc gccgcgggac tcgaccccaa cacctggttc tacaacgtcg aactcatcgc
     1921 cgcgcgtgaa atcggccgtg agaccgtcca gtacgtcagc aacgtcttca agtactacat
     1981 cgcgtacaag ctcgtcgtcg aacgggcgcg cgaacgggcc gccatcaaaa gacaggccgg
     2041 ctgagaaatt ccacggcgcc tcccacgccg gcttccacgc gcggcgcgca tcgttcgcgc
     2101 cgtcaacaac acctcagttt ggagtcgatc atgttcaagc cgacattcac ggcgctgggc
     2161 ctcgtggcct gcgccttcat ccaacctgcg caggcaggcc cctcgcagta tcgcgaaaac
     2221 gatccggccg tcatcaccga gtggaattcg attgccgaaa gcgtcattcc cgcttcggct
     2281 ggcgtcacgc tgccgcgcac ctacgcgatg atgcacatcg ccatgttcga cgcggtgaac
     2341 tcgatcgatg gcggatacac cgcctatcac gtgcgcatcc cggcatggga tggcgcttcg
     2401 agcgaagccg ccgcggcgca ggctgcccac gacgtgctga ccgcgctgct gccggccggc
     2461 acggcgacct acgatgccgc gctcgccgcg cgcctcgcca agatccattc gggccgcgcg
     2521 tatcacggtg cgagcgtcgg ccgcgaagtg gcgaagaaga tcctggaatg gcgcagcacc
     2581 gatggctggt cgacgccgca gaccttcacg ccgccggcgc tgcccggcgt gtggcaaccg
     2641 acaccgccgg ctttcgccgc cgcggccttc gtgcaggcgg gagatgccag gcctttcgcc
     2701 ctgcccactc cttattacta cctgccgcgc cgcccgccct cgctgaacag ccaggaatac
     2761 gcggatgccg tgaacgagat caaggctatc ggcggcgcca ccagcaccgt gcgcacggcc
     2821 gaacagacgt tgcaggctcg cttgtgggcc agcgtcggat acaaggacct gtggagcagc
     2881 gtgtggaatc aggtcacgcg tttcatggcg acgaaccgca acctgtcgat cggcgagtcc
     2941 gcgcgtctgt tcgcactggt gaacgccagc atgcaggacg gtgtgcagac cgcgcaggcc
     3001 agcaagttcg tgttccagct ctggcgcccg gtgacggcca tccagcgcgc gggtgaagac
     3061 ctgaacccga tgacggatgc cgatccgacc tggatgccgc tgctaactac tccgccctat
     3121 ccttcctatg cgggcaacat ggcgtgtatc ggtgcgagca cggcgcgcgc gctggcgctg
     3181 tacttcggca cgaacgacct gccattcacg gtgcagtgga ccggcctcaa cggcaatgcc
     3241 gacgtggcgc gtccgttccc ggggttctgg cagctggcgg aacaccaggg cgcgagccgt
     3301 gagtacggcg gcattcatta ccatttcgat acgacggcga gccaggagac ctgcccgaag
     3361 gtggcgggct acgtcttcgc gaattacatg cgtccgaaaa aggactggta acccgcggtt
     3421 acgcgcaata gcacttccct gaagacattg gcgcctcgaa agaggcgcct tttttttggc
     3481 ccttcacggg cccagggttt caggtcggag gccgccgcgg cgtgctgctg gcccaggccg
     3541 gaggggcgcc cgaggcgccg agttcggtgc tcgccttgcg caacgacgcc accagcgcgt
     3601 tgatctgcgg aatctgctcc tggtgcggtg ccgaagaaag acgctctttc atgcgttcca
     3661 gcgtttcggc gaaatcgcgc aggcgtttgg acgcattcac atggtcttcc atggatgcgg
     3721 tcaccgattt ttcgatgttg tgcaggtgcc ctagcatgcg ttccagggtt ccggcgtcgc
     3781 tgccccgcgg atcgcgggcc tgttcgtgtt tgaagactac ccggagcaga ttgcgggcct
     3841 gttctacggg atttggctgt cgttcgaggt ctgcgttcat gtggccggaa cagtggcagc
     3901 cgtaagcgga gttgtctatc ggaatttcag cctcctgaag gctcgcaacc tcgcgatctg
     3961 gtaagaattt tcaaactcgc accggcggcg agcagcgcgg ccagcggaca ccggccgtcg
     4021 ccgcgcttga tatttcgata atcgtcgtac tatcgtacgg ccccatgaaa acccctgaag
     4081 tcatcggcgc gctcggcgct ctcgcgcacg aataccgcct cgcgatctat cgacttctcg
     4141 ttgaacaagg cccggcgggt ctccctgccg gcgtcatcgg cgaacgcgtg ggattggtgc
     4201 cctcgtcgct cacgtttcat ctgaagtccc tgcaaagcgc cgggctcgtc acgcagcttc
     4261 gcgccagccg gcagctcatc tacagcgccg acttcgccgt gatgaacgca ctggtgggct
     4321 atctgaccga caagtgctgc agcgccggcg agtcctgcgc cgtggagtgc aagccggcgc
     4381 ggccgcgacg aaggaacgcc gcttgagcat catcagcgca cgtccttcgc tggcggccgt
     4441 ccagtcactg ctggagtcgg cgcaattgcc cacagcggat ctcaccgccg atcattgcga
     4501 gcattttttc tacctgggtt cgccggctac tcccgcgggc ctcgtgggcc tggagttgtt
     4561 cggagacgtc gcgctgcttc gttcgctggc cgtgaccccg gccgatcgcg cttccgggaa
     4621 cggttcggcc ttgctggaac atgccgaaac ctacgcacgt tccgccggcg tgaaatttct
     4681 ctacctgctg acgaccacgg ctgaagattt ctttgcgcgt cgcggataca cgcgaaccgc
     4741 gcgggatgcg gctccgccgg ccatcagcac cacgcgcgaa ttcgccggca tctgcccggc
     4801 cagctccgcg ttcatgtcca aaccactttg acgggaagcc aaaaatgtcc gaacaacctt
     4861 tcaacgtcct cattctctgc accggcaact ccgcgcgcag cattctcggc gaggccctca
     4921 tcaatcactg gggccgaggc aagttcatcg gttacagcgc gggcagcact cccaagggcc
     4981 aggttcatcc catcgcgctc gacctgctcc ggcaattgaa gatgcccacc gacggattgc
     5041 gcagcaagag ctggaatgag tttgcggcgc cgggcgcacc caggctggat ttcgtgttta
     5101 cggtctgcga caacgccgcc ggcgaatcct gtccgtactg gccaggacaa ccgatgaccg
     5161 cgcattgggg cgtcgaagac cccgccgcgg tcgaaggcac cgacaccgaa aagtggctgg
     5221 ccttccgcac cgcattccgc gaactcgaga accgcatcaa ggtgttcacc gcgctgccca
     5281 tccgcacgct ggatcgcgcg aagctccagg ctcatctgca cgagatcggc ggccgttccc
     5341 tgaaagaagt cggctgatgc aacgcgcggt ggcagcggaa tttctgggcg ccctgctgct
     5401 cacggccgcc gtcgtcggct cgggaatcat gggtgagcag ctcgccggcg gtaatgccgc
     5461 cgtggcgctg ctcgccaata ccggcgcgac cgtcgccgcg ctcgccacgt tgatagccgt
     5521 gttcggtcct gtgagcggcg cgcatttcaa tcccgtcgtc accctggtcg cgttccatca
     5581 gcgccagctg accctgcctc gaaccgtggc cttcatcggc gcccaggttg ccggctgctg
     5641 cgcaggcgca atgctggcca atgtcatgtt cgaactaccg gcgatcgccg cctcgacgca
     5701 cgtgcgcacc ggcagttctc agtggctttc agaagtcgtt gccaccgccg gcctcgtgta
     5761 catcgcgttc agctcgccgg cgtctgccgc cgcgtggcgc gttcccgcgt ggatcggcgc
     5821 cgcgtactgg ttcacggcct ccacttcctt tgcgaacccg gcgatcactc tcggc
//
LOCUS       DATDEU010000193         2777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_178911, whole genome shotgun sequence.
ACCESSION   DATDEU010000193 DATDEU010000000
VERSION     DATDEU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178911"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VIV63_04720"
     CDS             complement(<1..559)
                     /locus_tag="VIV63_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6123933.1"
                     /translation="MNALRAFEASARLGSFVAAATELRVSPAAVSQQVRRLEEYLDTT
                     LFQRLARGLTLTDQGRDYLPELSAGFDLLGESTARVRAKRADGVITVTTLAAFANGWL
                     LPRLHRFRERAPRIDVVLRTSRQVMDFRRDAIDLAIRFAPAPGRGLQGELPSREELFP
                     VASPQLFRGGCIPDTLSALVEYPLLH"
     gene            681..1289
                     /locus_tag="VIV63_04725"
     CDS             681..1289
                     /locus_tag="VIV63_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6123934.1"
                     /translation="MHLYDYLDSGNGYKVRLLLAQLGQKYDWTDVDIDKGLTRTPEFL
                     KRNPNGRIPTLELDDGTNLPESNAILWYLAEGSRFVPATQIGRAQVLQWMFFEQYSHE
                     PYVATPRYICRHFAADHPRRAELPDRIAKGRAAFAVMEQHLSTRPFFVNDRYSIADIA
                     LYAYTHVAEDGGHNLEAYPNMREWLGRVAGQPDYVPLLARPK"
     gene            1286..1627
                     /locus_tag="VIV63_04730"
     CDS             1286..1627
                     /locus_tag="VIV63_04730"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY6123935.1"
                     /translation="MSAAGFARTPAPPYYAVIFTSTRTTVDEGYGAMADRMVELAAKQ
                     PGFLGVESTRGADGLGITVSYWESLEAISAWKAVAEHRVAQSSGHKKWYEHFETRVAR
                     VERAYSGPRTT"
     gene            1742..2200
                     /locus_tag="VIV63_04735"
     CDS             1742..2200
                     /locus_tag="VIV63_04735"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6123936.1"
                     /translation="MRKFLFLNSAQRFSDFTLLLMRLFVGLFLIWGVWDNITDSARMQ
                     EFVAFLTKHDFAAPKILAPVSVYLQFAIGLGFVLGLFTRWAGIACVVVFVVAIVMVDR
                     FGGMRGIFPSGCLVAIGLYLATYGAGRFSVDAAMKANEVPRANGGVRFKH"
     gene            2213..2731
                     /locus_tag="VIV63_04740"
     CDS             2213..2731
                     /locus_tag="VIV63_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEY6123937.1"
                     /translation="MARNIEIKARIENVALLAPKVAEVASEGPLEIAQDDTFFKCDTG
                     RLKLRALSNDFGELIFYRRVNQAGPKESFYLRSPTSSPENLRESLSLAYGQIGRVRKY
                     RTLFLVGRTRVHLDRVEDLGHFLELEVMLVDDETPEQGILEASQLMDRLGIQPEQLID
                     GAYLDLLLAQSG"
BASE COUNT          486 a          906 c          883 g          502 t
ORIGIN      
        1 catgcagcag cgggtattcg accagtgccg acagcgtgtc cgggatgcag ccgccgcgaa
       61 aaagctgcgg gctcgcgacc ggaaacagct cctcgcggct cggcagttcg ccctgcaggc
      121 cccgtcccgg tgccggggcg aaccggatgg cgaggtcgat ggcgtcgcgg cggaaatcca
      181 tgacctgtcg cgacgtccgc agcaccacgt cgatgcgcgg cgctcgctcg cgaaaccggt
      241 gcagacgcgg caacagccag ccgttggcga aggcggcgag cgtggtcacg gtgatcacgc
      301 cgtcggcgcg cttggcccgc acgcgggccg tggactcgcc caacaggtcg aagcccgccg
      361 acagttccgg tagatagtcg cggccctggt cggtgagcgt gaggccgcgc gccaggcgct
      421 ggaacagcgt ggtgtcgaga tattcctcga ggcggcgcac ctgctggctc acggcagcgg
      481 gcgacacgcg cagttccgtg gcggcggcga cgaagctgcc gagccgcgcg gaagcttcga
      541 aggcacgaag tgcgttcaag ggtggaagcc gtcttgccat gccagagcaa gaataactta
      601 gcctcaggga aaagccttgg ctgtcgcgcc ttttgccggg cggccggata cttcgttgcc
      661 agacacacgg gaagcgatcc atgcacctct acgactatct cgactccggc aacggctaca
      721 aggtgcgcct gctgctggcg cagctcggcc agaaatacga ctggaccgat gtcgatatcg
      781 acaagggcct gacgcggacg cccgagtttc tcaagcgcaa tccgaacggg cgcattccga
      841 cgctcgagct cgatgacggc acgaacctgc ccgagtccaa cgccatcctc tggtatctcg
      901 ccgaaggctc gcgcttcgtg ccggccacgc agatcggccg cgcgcaggtg ttgcagtgga
      961 tgttcttcga gcagtacagc cacgagccct acgttgccac gccgcgttat atctgccggc
     1021 acttcgcggc cgatcatccg cgccgcgccg agctgccgga tcgcatcgcc aagggccgcg
     1081 ccgcgttcgc agtgatggag cagcacctgt cgacccggcc atttttcgtg aacgaccgct
     1141 acagcatcgc ggacatcgcg ctctacgcgt acacgcacgt ggccgaagac ggtggtcaca
     1201 acctcgaggc ctatccgaac atgcgcgagt ggttagggcg tgtggccggc cagccggatt
     1261 acgtcccgct gctcgccagg ccgaaatgag cgcagcgggt ttcgcgcgta ccccggcgcc
     1321 gccgtactac gcggtcatct tcacctccac gcgcacgacc gtggacgagg gttacggcgc
     1381 aatggccgac cgcatggtgg agcttgcggc caaacaaccc gggtttctcg gggtggagag
     1441 cacgcggggt gccgacggcc tcggcattac ggtgtcttac tgggagtcgc tcgaggccat
     1501 ctccgcctgg aaggcggttg ccgaacatcg ggtcgcgcag tcgtcgggcc acaagaagtg
     1561 gtacgagcat ttcgagaccc gggtggcccg ggtcgaacgg gcttacagcg gtccgcgcac
     1621 cacgtagcgt cgctacccgg cgacgtctac tcaagtccca cttgtacgac gactattggt
     1681 tgtcgcgccc cataacgtta gtcttccggg ccgatcacca tttccgggcc cgaggggact
     1741 catgcggaag tttctgttcc tgaattccgc gcagcgcttt tccgacttca ctctcctgtt
     1801 gatgcggctg ttcgtcggcc ttttcctcat ctggggcgtc tgggacaaca tcaccgatag
     1861 cgcgcgcatg caggaattcg tggcttttct gaccaaacac gacttcgcgg cccccaaaat
     1921 ccttgccccc gtctcggttt acctgcaatt cgccatcggc ctcgggttcg tgctcggcct
     1981 gttcacacgc tgggccggca tcgcctgcgt cgtcgtcttt gtcgtggcca tcgtcatggt
     2041 cgaccggttt ggcggtatgc gcggcatctt cccgtcgggc tgcctggtcg ccatcgggct
     2101 gtacctcgcg acctacgggg ccggccgttt ctcggtggac gcggccatga aggccaacga
     2161 ggtcccgcgc gccaacggtg gcgtgcgctt caagcactga cgaggcgcca ggatggcccg
     2221 caacatcgag atcaaggccc gcatcgaaaa cgtggcgctg ctcgcgccga aagtggccga
     2281 ggtggcgagc gaagggccgc tcgagatcgc ccaggacgac accttcttca agtgcgatac
     2341 cggccgcctc aagctgcgcg ccctgtccaa cgattttggc gagctcattt tctatcggcg
     2401 agtgaaccag gcggggccga aagagtcctt ctatctacgg tcccccactt cgtcacccga
     2461 gaatcttcgt gaatcattat cgctggccta cggacaaatc ggtcgcgtcc gcaaataccg
     2521 cactctcttc ctggttggac gtacccgcgt tcacctggac cgcgtcgagg acctgggcca
     2581 ttttctcgag ctcgaagtca tgctggtgga tgacgaaacc ccggagcagg gaattctcga
     2641 agcgagtcag ctcatggacc ggctcggcat tcagcccgag cagctgatcg acggcgccta
     2701 cctggacctg ctgctcgccc agtcgggctg aatggggaca tggataaatg gggacactcc
     2761 ccatttgcta gtaaaag
//
LOCUS       DATDEU010000194         2564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_178981, whole genome shotgun sequence.
ACCESSION   DATDEU010000194 DATDEU010000000
VERSION     DATDEU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178981"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VIV63_04745"
     CDS             <1..405
                     /locus_tag="VIV63_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6123938.1"
                     /translation="RVMYRIFVDWLRRTTRSPVVFAGRSGSTGAFPEDEEDGDDDQAS
                     DETWNPETLTEQAFERERIEEAWSHLQKQHRVVLSLHEIEGYSLEEVAQIIEVPLGTA
                     KSRLSRARNRLRALLTEGTNPPAPTCNPSETS"
     gene            436..954
                     /locus_tag="VIV63_04750"
     CDS             436..954
                     /locus_tag="VIV63_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123939.1"
                     /translation="MRARLSAMTVPEPSRGFEDRVLAKASGREPPQVRGFRAAIRRPA
                     TWWAAGAGALAASVAWMALLFVQSGGVREPNLVLALNESRDVPLVIDAERNLDDATIR
                     LSVSGSVGLAGFGEQQQIEWTTSLTQGANLVSLPVFARSPGNGLVVAEIEHEGRTRRL
                     SVALQVVRIGEG"
     gene            961..1410
                     /locus_tag="VIV63_04755"
     CDS             961..1410
                     /locus_tag="VIV63_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123940.1"
                     /translation="MNSEGIMRGARRLYLASLLALIVPAVFAQSPPASNRDELDVTMH
                     VIVDPDAKLPDEIVRRIPLPKPAQPAQPATGTDTTKPGKPSEPGAPGQQRGTDAREKG
                     REFGQQVSEDAKQRSEEARNNNKKPPPERPGRPPPPSPPGRPPSPGG"
     gene            1523..>2564
                     /locus_tag="VIV63_04760"
     CDS             1523..>2564
                     /locus_tag="VIV63_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6123941.1"
                     /translation="MILYEQSVALGMQGPAVHYNIGVAAYRKGDLARAQSAFEEVART
                     PAMAALAHYNLGLVALKRGEQKAARQWMERALRETSDEKLTTLAQNRLAELPEAVPSA
                     EWSLYARSGVGYDDNVALRSGSIDTIGSGQDDAFGELMLAGSYSFLPSWRVDGAVGLL
                     RYADLDDFDQDALSVGVVHGMTFNAWLLEPGVFASQYSLGGDVYERSVAASFDATRPL
                     FGHGMLRAQLRFSAVNGDGDFSGLTGSRSELGLKYEWGQQSWRFGAHARTELNNSEDD
                     VFASRWLEFGADARWAATPLWTFVAGATLRQTRHPAQSTTQDAWDDDRTVLRLEATRL
                     LWQHVQLYVRYQH"
BASE COUNT          466 a          795 c          862 g          441 t
ORIGIN      
        1 cgcgtgatgt accggatatt cgtggactgg ctgcgccgca cgactcgctc cccggtggtg
       61 ttcgcgggcc gcagcggctc gacgggcgcg tttcccgaag acgaagaaga tggcgacgac
      121 gaccaggctt ccgacgagac gtggaacccg gaaaccctta ccgaacaggc tttcgaacgc
      181 gagcgaatcg aggaggcgtg gagccacctg cagaagcagc accgcgtggt gttgtcgctg
      241 catgaaattg aggggtacag tctggaagag gtggcgcaga tcatcgaggt cccgctcgga
      301 accgccaagt cccgcctgag ccgcgcgcgt aaccgcctcc gcgcattgtt aaccgaggga
      361 acgaatccac ctgctccgac gtgtaatcct tcagagacat cttgagacta caacgtggtt
      421 gatgatcttc aaaaattgcg ggcgagactg agcgcgatga cggtgcccga gccgtcgcgt
      481 ggcttcgaag atcgcgtgct ggcgaaggcg tcgggtcgcg agccgcccca ggtgcgcggc
      541 tttcgcgctg ccatccggcg gccggccacc tggtgggctg cgggcgccgg tgcgctcgcc
      601 gcctcggtgg catggatggc gctgttgttc gtgcaatcgg gcggtgtgcg cgagccaaac
      661 ctcgtgctgg cgctcaatga aagccgtgat gttccgctgg tcatcgacgc ggagcgcaat
      721 ctcgacgacg ccaccatccg gctgtcggtg agcgggagcg tcgggcttgc cgggttcggc
      781 gagcagcagc agatcgaatg gaccacctcg ctcacgcagg gcgccaacct ggtgtcgctg
      841 ccggtgttcg cgcgctcgcc gggcaacgga ctggtggtgg cggagatcga gcacgaaggc
      901 cgtacgcgtc gcctgagcgt ggcgttgcaa gtcgtgcgga tcggtgaagg atgacatcgc
      961 atgaatagtg agggcatcat gcggggtgcg cgtcgcctgt atctcgcttc gttgctggcg
     1021 ctcatcgtgc cggcggtgtt tgcgcagtcg ccgccagcga gcaatcgtga cgaactcgac
     1081 gtcacgatgc atgtcatcgt ggatccggat gcaaaattac ccgacgaaat cgtgcgcaga
     1141 attccgctgc ccaagccggc gcaacctgcg cagccagcca ccggcacgga cacgaccaag
     1201 cctggcaaac cgagcgagcc cggcgcgcca ggtcagcagc gcggtacgga cgctcgcgag
     1261 aagggtcgcg aattcggtca gcaggtttct gaagacgcga aacagcgctc cgaagaagcg
     1321 cgaaacaaca acaagaaacc tccacccgaa agacccggca gaccaccgcc tccctcaccg
     1381 cctggtcgtc cgccctcgcc gggcggttga gttttttgcg ctcctgcttt tcagcagcgg
     1441 agcgcatgca gccgtcaccg aagcccaggc tcgcttcgcc gaagcgcgca ccgcattcga
     1501 ggcccaggat ttttcgcgcg cgttgatcct gtacgaacag agcgtggcgc tcggtatgca
     1561 agggcccgcg gttcattaca acatcggcgt cgcggcgtat cgaaagggcg acctcgcgcg
     1621 cgcgcagagc gccttcgagg aagtcgcccg aacgcccgca atggcggcgc tcgcgcacta
     1681 caacctcggc ctggtcgcgc tgaagcgcgg cgaacagaaa gcagcgcgcc aatggatgga
     1741 gcgcgccttg cgcgaaacct ccgacgagaa actcacgacg ctggcgcaaa accgtctcgc
     1801 cgaactaccg gaagccgtgc cgagtgcgga gtggtcgttg tatgcgcgca gcggcgtggg
     1861 gtacgacgac aacgtggctt tgcgctcggg gtcgatcgat acgatcggca gcggacagga
     1921 cgacgcgttc ggtgaactca tgctggcggg tagttattcg ttcctcccgt cctggcgtgt
     1981 cgacggcgcc gtgggattgc tgcgttacgc cgatctcgat gacttcgacc aggacgcgct
     2041 gtcagtcggc gtggtccatg gcatgacgtt caatgcgtgg ttgctggaac ccggcgtctt
     2101 cgcatcgcag tattcgctgg gcggcgatgt ctacgagaga tccgtggccg cttcgttcga
     2161 tgcgactcgc ccgctgttcg gtcatggcat gttgcgtgcg cagttgcgct tcagcgccgt
     2221 caatggtgat ggcgacttca gcgggctcac gggctcgcgt tcggagctcg ggctcaagta
     2281 cgaatggggc cagcagtcgt ggcgcttcgg cgcgcacgca cgcacggaac tcaacaacag
     2341 cgaagacgac gtgttcgcct cgcgctggtt ggaatttggc gcggacgccc gctgggcagc
     2401 aacaccgctg tggaccttcg tcgcgggcgc caccctgcgt cagacgcgac atccggcgca
     2461 gtccacgacc caggatgcct gggacgacga ccggacggtg ttacgactcg aagcgactcg
     2521 attgctctgg cagcacgtac agctctacgt gcgatatcag cacg
//
LOCUS       DATDEU010000195         4996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1347059, whole genome shotgun sequence.
ACCESSION   DATDEU010000195 DATDEU010000000
VERSION     DATDEU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347059"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VIV63_04765"
     CDS             <1..502
                     /locus_tag="VIV63_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6123942.1"
                     /translation="LLALNAAVEAARAGEQGRGFAVVATAVRTLAGRSATAAKEIKSL
                     IQDSVARVDAGSKLVDESGRTLEDIVNAVKKVTDIVAEIAAASREQSSGIEQVNRAVM
                     QMDQTTQQNAALVEEASAASQAIVEQAQALNVMIARYNVGDGSARAPRSSNRSSIADE
                     HVAHVA"
     gene            524..1045
                     /locus_tag="VIV63_04770"
     CDS             524..1045
                     /locus_tag="VIV63_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY6123943.1"
                     /translation="MSQSTAAVEPTAVQANAPGSSQYVTFVCAGVEYGVEILRVQEIK
                     GWDGVTRVPYTPPYVLGVMNLRGVIVPVIDLRARFGLESKAFDSSTVVIVVRVDAAVG
                     EKTVGIVVDAVSEVYNVAADAVKPTPDLGASADAACVSGLTSVDDKMVMLLDMNKLVA
                     SSIDLSAPSQMAD"
     gene            1065..1931
                     /locus_tag="VIV63_04775"
     CDS             1065..1931
                     /locus_tag="VIV63_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY6123944.1"
                     /translation="MNAGDTSEFAMAGADYELTDSQFHVIRGLVREHTGISLSDAKRQ
                     LVYGRLVRRLRALKLDSFGEYIELLERGVATELEEFTNAITTNLTSFFREPHHFEYLA
                     SDVLPQIVAKDSGLRRARIWCCAASTGEEPYSIAMVLREAQELLTGFDIKLLATDLDS
                     AVLATASAGVYNAERLKSVSGARASRFFTKGSGAQAGKFRAQDELRKLITFKQLNLMN
                     EWPVRGPFDAIFCRNVIIYFDKETQRVLFERMAALQRPGDILFLGHSESLYRVSDKYE
                     LIGRTIYRRNAA"
     gene            1952..3061
                     /locus_tag="VIV63_04780"
     CDS             1952..3061
                     /locus_tag="VIV63_04780"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEY6123945.1"
                     /translation="MGNKKIRVLVVDDSALVRQILVEVLNAARDVEVVGTASDPFMAR
                     DRIKELNPDVLTLDVEMPRMDGLTFLANLMRLRPMPVVMVSSLTEQGAETTLKALELG
                     AIDFVSKPKIDLAGTLADFGEEILAKIRIAAGARVMARSAPITAGRVAPKYSADAVLP
                     AGSGGKMLRTTDRIIAIGASTGGTEAIREVLMGLPADCPAIVIAQHIPEAFSLPFTRR
                     MDSLAAMSVVEPSDGQYIMAGHVYIAPGAKHLLVERDGARYRCRLNNGPPVNRHCPSV
                     DVLFRSVAQNVGPNAVGVILTGMGDDGARGLKEMLDAGARTMAQDEASSVVWGMPGAA
                     VRLGGAQDVLPLSRVAEHIMRLAQSVGAPAIAAAS"
     gene            3076..3438
                     /locus_tag="VIV63_04785"
     CDS             3076..3438
                     /locus_tag="VIV63_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6123946.1"
                     /translation="MARLIMTVDDSTSMRQMVKATLQSAGYDVVEAADGQEALDFARE
                     NPVDLVITDVNMPRMDGITLVSELRLLPTYRLTPVLLLTTESSMDKKMEGKKAGATGW
                     LVKPFNPAQLLATLTRVL"
     gene            3447..3923
                     /locus_tag="VIV63_04790"
     CDS             3447..3923
                     /locus_tag="VIV63_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123947.1"
                     /translation="MSELANTPVSALLELASSQLEVAAHTTDSQVATLANSIAALVTL
                     GAELQKVGAGTASDALSRKIVEQAQSALVAMQFHDQLVQRVAHVRDALTELNEALSGA
                     PVAEVDWATVLAGIRFRYSMEDERMLFDRIIGAAACPDMNAHDSLHDSLRGSVELF"
     gene            3923..4618
                     /gene="cheD"
                     /locus_tag="VIV63_04795"
     CDS             3923..4618
                     /gene="cheD"
                     /locus_tag="VIV63_04795"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF010013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HEY6123948.1"
                     /translation="MNAPVTAHTRLPQHARRVRTAPDNSAPPPALPQFSHVSRYWDTQ
                     NKCFAARLLPGEYYVTTQHEMIATVLGSCVSACIRDTRIGVGGMNHFMLPLDASQGNS
                     AWGAAVSAATRYGNVAMERLINDILKLGGRRENLELKLTGGGRVLAAMSTDIGARNIE
                     FVRQYVKDEGFNVVGEDLGDIYPRKVHYFPDTGKIRVKRFAAAKDETVVERERSYLNE
                     LDSTPIGGEVELF"
     gene            4637..>4996
                     /locus_tag="VIV63_04800"
     CDS             4637..>4996
                     /locus_tag="VIV63_04800"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY6123949.1"
                     /translation="MQTRLPDTRSAVRPETRMRDFTAMLEVEIAGDHIDLPSFPEIAL
                     RVRRALSDEDASIDHVVRVVSAEPSLVVRLLQLGNSAALNPSGRRLTDLRAAITRIGF
                     NMARSATIAFAMSQLRRA"
BASE COUNT          913 a         1622 c         1653 g          808 t
ORIGIN      
        1 tctgctggcg ctcaatgccg cggtggaagc cgcgcgtgcc ggcgagcagg gtcgcggctt
       61 cgccgtggtc gccaccgcag tccgcaccct cgcgggacgc tcggcgacgg cggccaaaga
      121 gatcaagtcg ctcatccagg acagcgtcgc gcgcgtggat gcgggcagca aactcgtcga
      181 tgaatccggc cgcacgctcg aagacatcgt gaacgcggtc aagaaggtca ccgacatcgt
      241 ggcggaaatc gccgcggcat cgcgcgaaca gtcgagcggc atcgagcagg tcaacagggc
      301 cgtgatgcag atggaccaga cgacgcagca gaacgccgcg ctggtggaag aggcgtcggc
      361 cgcgagccag gccatcgtcg aacaggcgca ggcgctcaac gtgatgatcg cgcgctacaa
      421 cgtcggcgac ggcagtgcgc gtgcgccgcg atcgtcgaac cgctccagta ttgcagacga
      481 gcatgtcgcc cacgtggcgt gacatcgaga ggaaaccgaa atgatgagtc aatctaccgc
      541 tgccgttgaa cccaccgccg tgcaggcgaa cgctccgggt agttcgcagt acgtgacttt
      601 cgtgtgcgcg ggcgtggagt acggcgtcga gatcctgcgc gtgcaggaga tcaagggctg
      661 ggacggcgtt acgcgcgtgc cctacacccc gccgtacgtg ctcggtgtca tgaacctgcg
      721 cggcgtcatc gttccggtga tcgacctgcg cgcgcgcttc ggtctcgagt cgaaggcctt
      781 cgacagctcg acggtcgtca tcgtggtgcg cgtcgacgcc gccgtcggtg aaaagaccgt
      841 cggtatcgtg gtcgacgcgg tgtccgaggt ctacaacgtc gccgccgatg cggtgaagcc
      901 gacgccggac ctgggcgcga gcgccgatgc cgcctgcgtg agcggactca cctcggtgga
      961 cgacaagatg gtgatgctgc tcgacatgaa caagctggtc gcctcgagca tcgacctgag
     1021 cgcgccatca cagatggcgg actgagcgcg acccaggcac gaccatgaac gccggagaca
     1081 ccagtgaatt cgcgatggcc ggagccgact atgagctcac ggactcgcag ttccatgtca
     1141 tccgcgggct cgtgcgcgaa cacaccggca tctcgctgtc cgacgccaaa cgtcagctcg
     1201 tctatggccg cctggtgcgg cgcctgcggg cgctgaagct ggattccttc ggtgagtaca
     1261 tcgagttgct cgagcgtggc gtcgccaccg agctcgagga attcacgaac gcgatcacca
     1321 ccaacctcac ttcgttcttt cgcgagcctc atcactttga atacctggcg agcgacgtgc
     1381 ttccgcagat cgtcgcgaaa gattccggcc tgcggcgcgc acgtatctgg tgctgcgcgg
     1441 cgtccacggg cgaggagcca tactccatcg ccatggtgct gcgcgaagcg caggaattgc
     1501 tgaccggctt cgacatcaag ttgctggcca cggatctcga ttcggcggtg ctcgcgaccg
     1561 cctcggccgg tgtctacaac gccgagcggc tgaagtcggt gagcggggcg cgcgcatcgc
     1621 gcttcttcac caaggggagc ggcgcgcagg cgggcaagtt ccgcgcgcag gacgaactgc
     1681 gcaaactcat caccttcaag cagctgaacc tgatgaacga gtggccggtg cgcgggccct
     1741 tcgatgccat cttctgccgc aacgtgatca tctatttcga caaggaaacg cagcgcgtct
     1801 tgttcgaacg catggccgcg ctgcagcgtc cgggcgacat cctgttcctg ggccactcgg
     1861 aaagcctgta ccgcgtcagc gacaaatacg aattgatcgg tcggactatc taccggcgca
     1921 acgccgcctg atggactccg ccggagactt tatgggaaac aagaaaatca gggtgctcgt
     1981 ggtcgacgac tccgcactgg tgcggcagat cctcgtcgag gtgctgaacg ccgccaggga
     2041 cgtcgaggtc gtgggtaccg ccagcgatcc gttcatggcg cgcgaccgca tcaaggagct
     2101 gaatccggac gtgctgacgc tcgacgtcga aatgccgcgc atggacgggc tcacgtttct
     2161 tgccaatctc atgcgcttgc ggcccatgcc cgtcgtcatg gtgtcctcgc tgaccgagca
     2221 gggcgccgag acgacgctca aggcactcga gctcggcgcc atcgatttcg tttccaagcc
     2281 gaagatcgac ctcgccggca cgctggcgga tttcggcgaa gagatcctcg ccaagatccg
     2341 cattgccgcc ggtgcgcgcg tgatggcgcg cagcgcgccc atcaccgcgg gacgggtcgc
     2401 gcccaaatat tccgccgacg ccgtgctgcc cgcgggctcg ggcggcaaga tgctgcggac
     2461 cacggatcgc atcatcgcca tcggcgcgtc cacgggcgga acggaggcga ttcgcgaagt
     2521 gctcatgggt ctgccggcag actgcccggc gatcgtgatc gcgcagcaca ttccggaagc
     2581 gttcagcctg ccgttcacgc ggcgcatgga ctcgctcgcc gcgatgtcgg tggtcgagcc
     2641 ttccgatggc caatacatca tggccggaca cgtctacatc gcgcccggcg cgaaacacct
     2701 gctggtggaa cgcgatggcg cgcggtatcg ctgccgtctg aacaacggac cgcccgtcaa
     2761 ccgtcactgc ccgagcgtgg atgtgttgtt ccgctcggtc gcgcagaacg tgggacccaa
     2821 tgccgtggga gtcatcctca ccggcatggg agacgacggc gcacgtggtt tgaaagagat
     2881 gctcgatgcc ggcgcgcgca ccatggcaca ggacgaagcg tccagcgtgg tgtggggaat
     2941 gccgggcgcg gcggtgagac tcggtggcgc gcaggacgtc ctgccgctca gccgggtagc
     3001 cgagcacatc atgcgactgg cgcaatccgt cggcgcaccg gccatcgcgg ccgccagtta
     3061 acgggagaga ggactatggc tagattgatc atgactgtgg acgattcaac ctcgatgcgg
     3121 cagatggtga aagccacgct gcagtcggcc ggctacgacg tcgtcgaagc cgccgacggg
     3181 caggaggcgc tcgacttcgc gcgcgagaat ccggtggacc tggtgatcac cgacgtgaac
     3241 atgccgcgca tggacggcat cacgctggtg agtgagctgc gtctgctgcc tacctaccgg
     3301 ctgacgccgg tgctgctgct gaccaccgag tcgagcatgg acaagaagat ggaaggcaag
     3361 aaggccggcg ccacgggctg gctcgtcaaa cccttcaatc cggcgcagtt gctggcgacg
     3421 ctcacgcggg tgctgtaagc ccgacgatga gcgaactcgc caacacgcct gtctccgcgt
     3481 tgctcgagct ggcctcgagc cagctcgaag tcgccgcgca cacgaccgac tcgcaagtgg
     3541 caacactcgc gaactcgatc gctgcgctgg tcaccttggg cgcggaattg cagaaggtcg
     3601 gcgccggcac ggcgtccgat gcgctttcgc ggaagatcgt cgagcaggcg cagagcgcgc
     3661 tggtggcgat gcagtttcac gaccagctcg tacagcgtgt ggcgcatgta cgcgacgcgc
     3721 tgacggaact caacgaggcg ctgagcggtg cgcccgtagc cgaggtcgac tgggccacgg
     3781 tgctggccgg catccgtttc cgctacagca tggaggacga gcggatgttg ttcgaccgga
     3841 tcatcggcgc cgccgcgtgt cccgacatga atgcgcacga ctccctgcac gacagcctgc
     3901 gcggaagcgt agagctgttc tgatgaatgc acccgtgacg gcccacacca gacttccgca
     3961 gcacgcccgg cgcgtccgga cggcgccgga caacagtgcg ccgccgccgg cgctgccgca
     4021 gttctcgcat gtgagccgct actgggacac ccagaacaag tgtttcgcgg cgcgcctgct
     4081 gccgggcgag tactacgtga ccacccagca cgagatgatc gccacggtgc tcggctcctg
     4141 cgtgtccgcg tgcattcgcg acacccgtat cggagtcggc ggcatgaatc acttcatgtt
     4201 gccgctcgat gcctcgcagg ggaacagcgc ctggggcgcg gccgtcagcg ccgcgacgcg
     4261 ttacggcaac gtggccatgg aacgcctcat caacgacatc ctcaagctgg gcggccgccg
     4321 cgagaatctc gaactgaaac tcacgggcgg cggacgcgtg ctcgcggcca tgagcaccga
     4381 catcggcgcg cgcaacatcg agttcgtgcg ccagtacgtg aaggacgaag ggttcaacgt
     4441 ggtcggtgag gacctgggcg acatctatcc gcgcaaggtc cattattttc ccgacaccgg
     4501 caagatccgc gtcaagcgtt tcgcggcggc gaaggacgaa accgtggtgg agcgcgagcg
     4561 cagctacctc aacgaactcg acagcacacc catcggaggc gaagtcgagc tgttctgaac
     4621 ggcccgaccc gcacccatgc aaacccgcct ccccgatacg cgcagcgcag tacgtccgga
     4681 gacgcggatg cgcgacttca cggcgatgct cgaagtcgag atcgccggcg atcacatcga
     4741 cctgccgagt ttccccgaga tcgcgttgcg cgtgcgccgc gcactctcgg acgaagatgc
     4801 ctcgatcgat cacgtcgtcc gcgtggtcag cgccgagccg tcactggtgg tccgcctgct
     4861 gcagctcggc aactcggcgg cgctcaatcc gagcggccgc cgcctgacgg atctgcgcgc
     4921 ggccatcacg cgtattggct tcaacatggc gcgcagcgcc acgatcgcgt tcgccatgtc
     4981 gcagcttcgc cgcgcg
//
LOCUS       DATDEU010000196         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1784780, whole genome shotgun sequence.
ACCESSION   DATDEU010000196 DATDEU010000000
VERSION     DATDEU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784780"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1357
                     /locus_tag="VIV63_04805"
     CDS             185..1357
                     /locus_tag="VIV63_04805"
                     /inference="COORDINATES: protein motif:HMM:NF020505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monodechloroaminopyrrolnitrin synthase PrnB
                     family protein"
                     /protein_id="HEY6123950.1"
                     /translation="MSNLSDAFDHWIRGSFMEINTELENLYFIQGDRADVTRCGAERK
                     SQLRDEGRLHVVALLAEGDTGDGFETAFGVLGNVGMYLAALRRHELTNPAREERSPFP
                     EASSLAMHVGAALGMAPRFSTAHIAHLNFSEAGVRKSFTSLPDEILFIDENTRGILLL
                     QLAADALNKVVPLGISSPIVDVPFAAARHALEAVIESNQRLFDKLDVERFFFSVRPYY
                     KPYKVGRQEYRGANAGDFSGINEIDLLLGLCRANDPYYAQLLVDKMQFMPPQDQARLR
                     ECMRHESFLDELLALAPAHAREDWFRQNARAYLGLCDLFGRYASQHHDDLVKRFIEAP
                     AARIDAAHLEGVTASGPPLPVLLRSLEVLRDMRLAANRADIATRHADLERLRKMVP"
     gene            complement(1354..2487)
                     /locus_tag="VIV63_04810"
     CDS             complement(1354..2487)
                     /locus_tag="VIV63_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123951.1"
                     /translation="MKTQGRLGWLAGLGLLAVSAVAIAAPPPVYKIEGVRITDIGTLG
                     GKWAVAWDINNSGNIVGSSKTSLGVDHAFLYKGGTMYDISPAFSLAGSEARAINQADT
                     VAANYGSSGTALGDHGYRWAGGVAYPLTDTDPAGIPGKSRVWGISDKGLIAGQRVALT
                     PASTVAAVWTSDTTYVPLTAPGGSPSYARDVNASGLIVGFQSSDGTPRRWKVLWGGIV
                     TSDIAPSPLPVSMFGIGGLEGVNKSGAVAGQISCCIGTPSVRRNAWTWNGSSAAGVRI
                     GLLWVGGFSQGEDINNNGIVAGYAQRPSPPDPYSDEWDAAILYHADFGIYQLPALSSF
                     FQSCRASALNDLSGGRLQVVGVCLHVLGEYRAVRWDVKVSQVP"
BASE COUNT          469 a          844 c          793 g          462 t
ORIGIN      
        1 agcgacttcg gccactgagc atcgtccggc tttccccaga gtgctgcgac gcgagctaac
       61 gcttcagcaa tccgctcgcc cttccccgga aacatgccga tggagtggta caggaagtag
      121 cccgaaccat cgtcgatgaa cttggccatt tgcagagcat gtcacgattc ggcaggctgc
      181 cggcatgagc aacctttcgg acgccttcga ccactggatc cgcggctcct tcatggagat
      241 caacacggag ctggagaatc tctatttcat acagggcgac cgcgctgatg tgacgcgatg
      301 cggcgcagaa cggaaatcgc agctgcgcga tgaaggtcgc ctgcacgtgg tcgcgctgct
      361 ggcggagggt gataccggcg acgggttcga aaccgccttc ggcgttctcg gcaatgtcgg
      421 catgtacctg gccgcgctgc ggcgtcacga gctcacgaac ccggcgcgag aggaacgctc
      481 gccgtttccc gaagcgtcat cgctggcgat gcacgtcggt gcggcgctcg gcatggcgcc
      541 gcggttttcg acggcgcaca tcgcgcatct caatttttcc gaggcgggcg tgcgcaagag
      601 tttcacgtcg ttgccggatg agatcctgtt catcgacgag aacacgcgag gcatcctgct
      661 gctgcaactc gcggccgatg cgttgaacaa ggtggtgccg ctcggtatct cgagcccgat
      721 cgtcgatgtt ccgttcgccg ccgcccggca tgcgctcgaa gcggtcatcg aatccaacca
      781 gcggctgttc gacaagctcg atgtcgagcg attctttttt tccgtgcgtc cgtattacaa
      841 gccttacaag gtgggacgcc aggaatatcg cggcgcgaac gcgggcgact tctccggcat
      901 caatgaaatc gatctgttgt tagggctgtg ccgcgccaac gacccgtact acgcgcagct
      961 cctggtcgac aaaatgcagt tcatgccgcc gcaggaccag gcgcgactgc gcgaatgcat
     1021 gcggcacgaa agttttctcg acgaactgct ggcgctggcg cccgcgcatg cgcgcgagga
     1081 ctggttccgt cagaacgcgc gcgcgtatct cgggctttgt gatctgttcg ggcggtacgc
     1141 cagccagcat cacgacgacc tcgtgaagcg cttcatagaa gcgcccgcgg cgcgcatcga
     1201 cgcggcgcat ctcgaaggcg tcaccgcgag cggcccgcca ctacccgtgc tgcttcggtc
     1261 gctcgaagtg ctgcgtgaca tgcggctggc ggcaaatcgc gccgacatcg ccacgcgcca
     1321 cgcagacctc gagcgcctcc gcaaaatggt gccctaagga acctgcgaca ccttcacatc
     1381 ccatcgtacg gcgcgatact cgccgagcac gtgcaggcac acgccgacga cctgcagccg
     1441 gccacccgaa agatcgttga gcgccgacgc gcggcagctt tgaaagaagc tcgacaacgc
     1501 cggtaactgg tagataccga aatccgcgtg atagaggatc gccgcgtccc actcgtcgct
     1561 gtacggatcg ggcggcgacg ggcgctgcgc atagcccgcg acgattccat tgttgttgat
     1621 gtcttctccc tggctgaacc cgccgaccca caacaacccg atgcgcacgc cggcggcact
     1681 cgatccgttc caggtccacg cattgcgccg cacggacggc gtgccgatgc agcacgagat
     1741 ctgcccggcg acggcgccac tcttgttcac cccttcgagg ccgccgatcc cgaacatcga
     1801 taccggcaat ggcgaaggcg cgatgtcgct ggtgacaata ccgccccaca gtactttcca
     1861 gcgtcgcggc gtcccgtcgg aactctgaaa cccgacgatc aatcccgacg cgttgacgtc
     1921 ccgggcgtaa ctgggcgaac caccgggagc cgtgagcggg acgtaagtcg tgtcactggt
     1981 ccagacggcc gcgaccgtcg acgcgggggt cagcgccacg cgctgcccgg caatcaaccc
     2041 tttgtccgaa attccccaga cgcgggactt gccgggaatt ccggccggat ccgtgtcggt
     2101 gagtggatag gcgactccgc cggcccatcg gtatccgtgg tcaccgagtg cggtgccgga
     2161 actgccgtag ttggcggcca cggtgtcggc ctggttgatc gcgcgcgctt cactgccggc
     2221 cagcgagaac gccggcgaga tgtcgtacat cgtgccgccc ttgtagagga acgcgtgatc
     2281 gaccccgagc gaggttttcg atgacccgac gatgttgccg ctgttgttga tgtcccaggc
     2341 cacggcccat ttgccgccca gcgttccgat gtcggtgatt cttacacctt cgatcttgta
     2401 aaccggcggc ggtgcagcaa tggcgaccgc cgatacggcg agcagaccca gtcccgctaa
     2461 ccaccccaac cttccctgag ttttcattgg catcctctcc atccgatctg ctgcccagcg
     2521 cagccggcga agaatggaag gggcgcgcca ggggcgcagt ggctagtc
//
LOCUS       DATDEU010000197         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_617951, whole genome shotgun sequence.
ACCESSION   DATDEU010000197 DATDEU010000000
VERSION     DATDEU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617951"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VIV63_04815"
     CDS             <1..685
                     /locus_tag="VIV63_04815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6123952.1"
                     /translation="KTPDGICDAHFVHPAKATAAVLVWPDIYGLRPAFRAMGKRLAES
                     GYAVLTVNPFYRSIKGAPASREDAFKYAGSLNATTHVSDAKAFVTWLDAQKAVDTKRK
                     IGTTGYCMGGAIVMRTAASLPDRIGAGCSFHGGRLTTADADSPHLLIPKMKASMLIAI
                     ADNDDQKEPASKDILRKAFDDAKVSAEIEVYKGSNHGWMPPDSQNHNATGAERGWERK
                     LALFKKALA"
     gene            complement(716..1525)
                     /locus_tag="VIV63_04820"
     CDS             complement(716..1525)
                     /locus_tag="VIV63_04820"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6123953.1"
                     /translation="MSAGKADEYLKIEHLCKTFGSGESRSEVLRDISLDVARGEYIAI
                     IGHSGCGKSTLLNIVAGLTEATSGYVFLEGREVHQPGPDRAVVFQNHSLLPWLTVYDN
                     VRLAVDKIFRGSKTSAERHEWTLRNLELVHMTQAMKKRPAEISGGMKQRVGIARALAM
                     SPKVLLLDEPFGALDALTRAHLQEQVMQIHADLGNTVLMITHDVDEAVLLSDRIVMMT
                     NGPAAQVGELHTVPLARPRNRLELIADASYIESRGKVLEFLHKKSHYVEAA"
     gene            complement(1522..>2122)
                     /gene="ntrB"
                     /locus_tag="VIV63_04825"
     CDS             complement(1522..>2122)
                     /gene="ntrB"
                     /locus_tag="VIV63_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HEY6123954.1"
                     /translation="LFWHLYASLKRVSLGYAFAASAGIALGVLIGSSKWALRALDPLF
                     QVLRTIPPLAWLPLSLAAFRDGQPSAIFVIFITAIWPIIINTAVGVRNIPQDYRNVSR
                     VLRLNGFEFFTHIMLPAAAPYIFTGLRIGIGLSWLAIVAAEMLIGGVGIGFFIWDAWN
                     SSLISDIILALVYVGIIGFLLDRAVAFTGKLVGRGTVSV"
BASE COUNT          417 a          705 c          653 g          347 t
ORIGIN      
        1 caaaaccccc gacggcatct gcgacgccca cttcgtgcat cccgccaagg ccaccgccgc
       61 cgtgctcgtg tggcccgaca tctacggtct tcgccccgca ttccgcgcca tgggcaagcg
      121 gctcgcggaa tccggctacg ccgtactcac ggtcaacccg ttctaccgtt ccatcaaggg
      181 agcgccggcc agccgcgagg acgccttcaa gtacgcgggc tcgctcaatg ccaccacgca
      241 cgtgtccgac gcgaaggcct tcgtgacctg gctcgacgcg cagaaggccg ttgataccaa
      301 gcgcaagatc ggcaccacgg gctactgcat gggcggagcc atcgtgatgc gcaccgccgc
      361 atcgctgccg gaccgcatcg gcgccggctg ctcgttccat ggcggtagat tgaccacggc
      421 cgacgcggac agccctcacc tgctgatccc gaagatgaag gccagcatgc tgatcgccat
      481 cgcggacaac gacgatcaga aggagccggc gtcgaaggac atcctgcgca aggcgttcga
      541 cgatgcgaag gtgtcggcgg agatcgaggt gtacaagggc tcgaaccacg gctggatgcc
      601 gccggattcg cagaaccaca acgcgacagg cgccgagcgt gggtgggagc gcaagctggc
      661 gctgttcaag aaggccctgg cgtagagagc tggcatcgat tgccgggcgt cgaactcacg
      721 cggcttcgac gtaatggctc ttcttgtgca ggaactcgag caccttgccg cggctttcga
      781 tgtacgaggc atccgcaatc agctccagtc ggttcctcgg cctggccagc ggcacggtgt
      841 gcagctcgcc gacttgcgcg gccgggccgt tggtcatcat cacgatgcgg tccgatagca
      901 agactgcctc atccacgtcg tgagtgatca tgagcacggt gttgcccagg tcggcgtgaa
      961 tctgcatcac ctgctcctgc aggtgcgccc gcgtgagcgc gtccagcgcg ccaaacggct
     1021 cgtccagcaa caacaccttc gggctcatgg cgagagcgcg tgcgataccc acgcgctgtt
     1081 tcatgccgcc ggagatttcc gccggccgct tcttcatggc ctgcgtcatg tggacgagct
     1141 ccagattgcg caaagtccac tcgtggcgtt cggcgctggt cttgctaccg cggaatatct
     1201 tgtccacggc gagacgcacg ttgtcataga ccgtgagcca cggaagcagc gaatgattct
     1261 ggaacaccac ggcgcgatcg ggccccggct gatgaacctc gcggccttcc aggaacacgt
     1321 agccgctggt cgcctcggtg agacccgcca cgatgttgag cagcgtcgac ttgccgcacc
     1381 cggaatgccc gatgatggcg atgtactcgc cccgcgccac atccagcgag atgtcgcgca
     1441 gcacttcact gcgcgattca ccgctgccga acgtcttgca gaggtgctcg atcttcagat
     1501 attcgtccgc tttgccggca ctcatacgct caccgtccca cgaccgacca gcttgcccgt
     1561 gaaggccacc gcgcggtcca acaagaagcc aatgattccc acgtacacca gcgccaggat
     1621 gatgtcgctg atcagcgagc tgttccacgc atcccagatg aagaagccga tgcccacgcc
     1681 gccaatcagc atctcggccg cgacgatggc gagccacgag aggccgatgc cgatgcgcag
     1741 gcccgtgaag atgtacggcg ccgcggccgg caacatgatg tgcgtgaaga actcgaagcc
     1801 gttcagtcgc aacacacgcg acacgttgcg atagtcctgc ggaatgtttc gcacgcccac
     1861 cgcagtgttg atgatgatgg gccagatcgc cgtgatgaag atgacgaaga tcgccgaggg
     1921 ctggccgtcg cgaaaggccg cgagcgacag cggcagccag gcgagcggtg gaatcgtccg
     1981 cagcacctgg aacagcggat ccagcgcgcg caacgcccac ttgctgctgc caatgagtac
     2041 accgagtgcg atgccggcgc tcgccgcgaa cgcgtatccc agcgacacgc gcttcaacga
     2101 ggcgtagaga tgccagaaca at
//
LOCUS       DATDEU010000198         6654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_909930, whole genome shotgun sequence.
ACCESSION   DATDEU010000198 DATDEU010000000
VERSION     DATDEU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909930"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..708
                     /locus_tag="VIV63_04830"
     CDS             118..708
                     /locus_tag="VIV63_04830"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY6123955.1"
                     /translation="MQNPIRALAFIIAGATASATFAAPVTYNVDPEHTFPSFEADHFG
                     GMSVWRGKFNQNSGKVVLDREAQTGTVDITIDTNSIDFGLEKLHTHVKGSDAGMLDVA
                     KFPTATYKGKLAKFKDGAPTEVQGELTLHGVTKPVTLTIRSFKCMAHPMKKKEFCGAD
                     AVGSFSRADFGVDWGKAFGFGMDVKLAIQIEAFKAD"
     gene            729..2084
                     /locus_tag="VIV63_04835"
     CDS             729..2084
                     /locus_tag="VIV63_04835"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY6123956.1"
                     /translation="MRKTFCALALLAFGMTAASADKDGATALFDGKTLSGWKTLGGGA
                     DYNVIDGAIVGSSRPGVPNSFLVTEKVYRDFIFEFDVRQDVGPTNSGVQFRSLSTPEF
                     ENGRVHGYQSDIDPSPRQWSGSIYEEAQRGWFYTGELHPPAKALYKFGEWNHYRIEAI
                     GPRLRVWINDSPAADVIDDVKKEGFFGLQVHSINSAEEAGRTTSWKNLQVQTKNLKPR
                     PPMEIFIRNNLPNNLDADEKAQGWRLLWDGKTTRGWRSANAEGFPAKGWSFADGALAV
                     QAKGGGGDIMTDEEFGAFELQMDFKVSAGANSGIFYLLTSPRDPASGAPLGLEYQILD
                     DELHPDAKLGIDGNRTIASLYDIYPRAKLMTNVGIAPKVDVWQHARIVARPDGTVEHW
                     LNGVKVLEFKRGSDDYRTHVAASKFKSTPGFGEAQKGRILLQDHGDAVAFRSIKVREL
                     K"
     gene            complement(2085..2849)
                     /locus_tag="VIV63_04840"
     CDS             complement(2085..2849)
                     /locus_tag="VIV63_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY6123957.1"
                     /translation="MHRAKGRKILKDRRAFLALGLAALGASLPAWAAEHPPPPKPKPK
                     PTGAAPAAKPPAQRARLPRRLAADFRSHIETRLPHLRSSFEAAGRDTGLRWRMLAALG
                     YQESRWRPAAVSPKGAQGVMMLMPVTATKMGVKNVFSPDENIMAGSRYLAYMKERIPK
                     RIRDPDRTLLAMAAYNIGIGHLEDARIITQMRKKNPDSWPDVRANLPRLSDPVWHSRV
                     KRGYANGRETVQFVERVSQFSAILEASVPEAVIARD"
     gene            2919..3011
                     /locus_tag="VIV63_04845"
     tRNA            2919..3011
                     /locus_tag="VIV63_04845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2954..2956,aa:Ser,seq:cga)
     gene            3202..4620
                     /locus_tag="VIV63_04850"
     CDS             3202..4620
                     /locus_tag="VIV63_04850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY6123958.1"
                     /translation="MDEARITWTPAERESFFDAIARHRRAAWQVSAVSLLASSIVALV
                     VALLLSPLFYVALSLVCDLANLFVPTINLAGIAFARISPMLDDPAGVTAAQWLGLAAF
                     AALPGLVFMGATLFVTRRAVILSGMLDGALFTAVAPDVLELEEQRFRNVVAEMAVAAG
                     LAEPRVLIADWPSSNGAVFGADDGSAVVVVSRQLIQTVDRAQLQAIAAHLVGSIANGD
                     MAIGVRVATTLGLFGLIARLGGAFTQRASMARLMRALARVTFRPDKAAARAYVDEIAQ
                     PFRQEDDPPGSATPATAQDWRAIAWLPFAGTIFATGFFGGIISLFALEPLVSLTWRRR
                     KFLADATAVRLTRDPNALACALSGTGGGETPLGVWGAHLCVVRPAARSGGLLGGSMVP
                     AIPATDRRLRALATLGADAAPAAKRLPMAKILIIAPLCLLVAVLLSAVLGLLVWLSVA
                     LSMMFLGLPFGALHLLLRWMGH"
     gene            4690..5205
                     /locus_tag="VIV63_04855"
     CDS             4690..5205
                     /locus_tag="VIV63_04855"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY6123959.1"
                     /translation="MTHAVAQHFCCTLDSPVGALSLVATDQALVAVVWRREGHRHVPF
                     ENAVEDPNHSLLRETQRQLREYFASKRRAFDLPLEFRGTDFQRRAWAALLTIPYGETR
                     TYRQMAEQIGNPTAVRAVGAANGRNPISIIAPCHRVIGMNGDLTGFGGGLDAKAHLLS
                     LEAPQRQLLAG"
     gene            complement(5222..6496)
                     /locus_tag="VIV63_04860"
     CDS             complement(5222..6496)
                     /locus_tag="VIV63_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6123960.1"
                     /translation="MGPQARARHRHADRSPAVRGGALTDEVAAVFRREYGRAVAVLVR
                     FLGDIGLAEESVQDAFTTAITHWPLTGIPASPAGWIITTARNRALDHLRREATRDARQ
                     DAAMLLLEQEAVEEHDVRDDRLRLIFTCCHPAIAPEAQLALTLRLLGGLSTAEIARAF
                     LVPEPTMAQRLSRAKAKIRDAGIPYRVPDNSELPARLGAVLAVVYLIFNEGYSATSGD
                     ALVRAELCDEALRLGRLLVELLPEESEAIGLLALMLLIDARRHARVDGAGEFVRLAEQ
                     DRRAWNRTRIEEGQTLLRVCLARNRPGPYQLQAAINAVHSDARSAADTDWQQILGLYD
                     HLLALAPSAVIALNRAVVVAELEGPERGLVLVDALDLPRYHLLHAVRADLLRRCGRDA
                     DAAVAYQAAIEQCGNERERRFLQRQYESIASH"
     gene            complement(6427..>6654)
                     /locus_tag="VIV63_04865"
     CDS             complement(6427..>6654)
                     /locus_tag="VIV63_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY6123961.1"
                     /translation="WIFGGGLTPPDSATVVRHQGGGLITTDGPFIESKEQIGGITILK
                     APDLDEALAWGRKLARAIGTPIEVRPFAEGH"
BASE COUNT         1127 a         2171 c         2180 g         1176 t
ORIGIN      
        1 ggcgggagct gacgtaacgg acggacgcgg cgccatagtt atttaggagg aggagatcga
       61 gaggtcggtg tgctagcgtc gcgctccttc cgccgcctct catgaaggat ttacaccgtg
      121 caaaacccaa ttcgtgcgct cgcgttcatc atcgctggcg ccactgcctc cgccacgttc
      181 gccgcacccg tgacctataa cgtggatccc gagcacacct ttcccagttt cgaagcggac
      241 cactttggtg gcatgtccgt gtggcgcggc aagttcaatc agaacagcgg caaggtcgta
      301 ctcgaccgcg aagcccagac cggaacggtc gacatcacca tcgacacgaa ctcgatcgac
      361 ttcgggctcg agaagcttca cacgcatgtg aaaggctcgg atgcgggaat gctcgatgtc
      421 gcgaaattcc ccaccgccac gtacaaggga aaactcgcca agttcaagga cggcgcgccg
      481 acggaagtgc agggcgaact cacattgcac ggtgtcacca agccggtgac gctcaccatc
      541 cgttcgttca agtgcatggc gcaccccatg aagaagaagg aattctgtgg cgccgacgcc
      601 gtgggcagtt tcagccgggc ggacttcggc gtcgactggg gcaaggcgtt cggcttcggg
      661 atggacgtga aactcgccat tcagatcgaa gcgttcaagg ccgattgatc taaaacgagg
      721 ggattcggat gcggaagact ttttgcgcgc tggcgttgct ggcgttcggt atgactgcgg
      781 ccagcgcgga caaggatggc gccacggcgc tgttcgacgg caagacgctg agcggttgga
      841 aaacgctggg cggcggcgcc gattacaacg tgatcgacgg cgcaatcgtc ggttcctcgc
      901 gtcccggagt gcccaacagc ttcctcgtca ccgaaaaggt ctaccgcgat ttcatcttcg
      961 agttcgacgt gcgccaggac gtcggaccga cgaactcggg tgtgcagttc cgcagcctga
     1021 gcacgccgga attcgagaat ggacgcgtgc acggctatca gtccgacatc gacccgtcgc
     1081 cgcgccagtg gagcggcagc atctatgagg aagcgcagcg tggctggttc tacaccggcg
     1141 aactgcatcc gccggccaag gccctctaca agttcggcga gtggaatcac tatcgcatcg
     1201 aagccatcgg tccgcgcctg cgcgtgtgga tcaacgacag tcccgccgcg gacgtgatcg
     1261 acgacgtgaa gaaggaaggc ttcttcggct tgcaggtcca cagcatcaac tcggcggaag
     1321 aggccgggcg tacgaccagc tggaagaacc tgcaggtgca gacgaagaac ctgaagccgc
     1381 ggccgcccat ggaaatcttc atccgcaaca acctgcctaa caacctcgac gccgacgaga
     1441 aggcgcaggg ctggcggctg ctgtgggatg gcaagaccac gcgcggctgg cgcagcgcca
     1501 acgcggaagg gtttccggcc aagggctggt cgttcgccga tggtgcgctc gcggtccagg
     1561 ccaaaggcgg tggcggcgac atcatgaccg acgaggaatt cggcgccttc gagctgcaga
     1621 tggacttcaa ggtgtccgcc ggcgccaaca gcggcatctt ctatctactg acttcgcccc
     1681 gggatccggc gagcggcgcg ccgctcggcc tcgaatacca gatcctcgat gacgagctgc
     1741 accccgacgc caagctgggc atcgacggca atcgcaccat cgcctcgctc tacgacatct
     1801 atccgcgcgc caagctcatg accaacgtgg gcatcgctcc caaggtcgac gtgtggcagc
     1861 acgcgcgcat cgtcgcgcgg cccgatggca cggtcgagca ctggctgaac ggcgtcaagg
     1921 tgctggagtt caagcgcggc tcggacgact accgcacgca cgtcgcggcc agcaagttca
     1981 agtcgacgcc gggtttcggc gaggcgcaga agggccgcat cctgctgcag gaccacggcg
     2041 acgcggttgc gttccgcagc atcaaggtcc gcgagctcaa gtagctagtc gcgggcgatc
     2101 acggcctcgg gcaccgaggc ttcgaggatg gccgagaact gcgacacgcg ctcgacgaac
     2161 tggacggtct cccggccgtt ggcatagccg cgtttcacgc gcgagtgcca caccggatcg
     2221 gaaaggcggg gcaggttggc gcgcacgtcg ggccagctgt cgggattctt cttgcgcatc
     2281 tgcgtgatga tgcgggcgtc ttcgagatgc ccgatgccga tgttgtaggc cgccatggcg
     2341 agcagggtgc ggtccggatc gcggatgcgt ttcgggattc gctccttcat gtacgccagg
     2401 tagcgcgagc ccgccatgat gttctcgtcg ggcgaaaaga cgttcttgac gcccattttc
     2461 gtcgccgtca ccggcatcag catcatcacg ccctgagcac ccttgggcga taccgccgcc
     2521 ggacgccagc gcgattcctg gtaacccaag gcggccagca tccgccagcg aagtcccgta
     2581 tcccggcccg cggcctcgaa tgacgagcgc aggtggggca gccgggtttc gatatggctg
     2641 cggaagtcgg ccgcgagccg cctcggcagg cgtgcccgct gcgccggggg tttggcggcc
     2701 ggcgccgctc ccgtgggctt gggcttcggt ttgggcggag gcgggtgttc ggcggcccac
     2761 gccgggaggg atgcccccag agccgcgagc ccgagggcca ggaatgcccg ccggtccttc
     2821 aggattttcc gccccttggc tctgtgcatc gttcggctag aatacgcgcc ccgcgaaggg
     2881 cggccctaat ttaagaggtt gtcccaaggt tcaggcgcgg agaggtaccg aagcggtcat
     2941 aacggcgccg actcgaaatc ggatggtcgg gtaattccgg cacgtgagtt cgaatctcac
     3001 cctctccgcc atttattctt tgtaccgtca tcgcgggtga gattcgaact cacgtgcggt
     3061 aaatgaggac gagctcgcgc tttgcagagc aaagatggac agattcgcct cctggcgaat
     3121 ctggtcccga gcaatctcac ccgtcctgcg agcaaccgcg tccctctgtc cgaatacttc
     3181 gccaatcacg tagtcctgtg catggatgaa gcacgaatta cgtggacccc cgccgaacgc
     3241 gagagtttct tcgacgccat cgcgcgtcac cgccgcgcgg cctggcaggt aagcgccgtg
     3301 agcctcctcg cgagctcgat cgttgcactc gtggtggcgc tgcttttgtc gccgctgttc
     3361 tacgtggcgc tgtcgctcgt ctgcgacctc gcgaacctct ttgtccccac gatcaatctc
     3421 gccggcatcg ccttcgcgcg tatctcgccg atgctggatg acccggccgg cgtcaccgcg
     3481 gcgcagtggt tagggctcgc cgcattcgcg gcgctgcccg ggcttgtctt catgggcgcg
     3541 accctcttcg tgactcgccg cgcggtcatc ctctccggaa tgctcgacgg agcgctgttc
     3601 acggccgttg ctcccgatgt cctcgagctc gaggagcagc gtttccggaa tgtggtcgca
     3661 gaaatggctg tcgccgcggg gcttgcggag ccacgcgtgc tcatcgcgga ttggccctcg
     3721 tcgaatggcg ccgtattcgg cgccgacgac ggcagtgcgg tagtcgtggt gtcgcgccag
     3781 ctcatccaaa ccgtggatcg cgcccagctg caggcgatcg ctgcgcacct cgtgggctcg
     3841 atcgccaacg gcgacatggc catcggagta cgcgtcgcaa cgacgctggg attgtttggc
     3901 ctgatcgcgc ggctcggcgg tgcattcacg cagcgcgcga gcatggctcg actgatgcgg
     3961 gctttggcgc gcgtgacatt ccggccggac aaggcggctg cgagagccta cgtcgacgag
     4021 attgcgcagc cgttccggca ggaggacgac ccgccgggca gcgccacgcc cgcgactgcg
     4081 caggactggc gcgccatcgc ctggctgccg ttcgccggga cgattttcgc gacgggattt
     4141 ttcggcggca tcatcagcct gttcgcgctc gagccactgg tgtcgctgac gtggcgacgg
     4201 cggaaatttc tcgcggatgc gacggccgtg cgactcactc gcgacccgaa tgcactggct
     4261 tgcgcattgt cagggacagg cggcggcgaa acgccgctcg gcgtgtgggg cgctcatctg
     4321 tgcgtcgtcc ggccggccgc gcgaagtggt ggcttgctcg gcggctccat ggtccctgcg
     4381 attcccgcca ccgatcggcg cctgcgcgct ctcgcgacgc tcggcgcgga tgccgcgccg
     4441 gccgccaaac gcctgccgat ggcgaaaatt ctcatcatcg cgccgctgtg tcttctggtc
     4501 gccgtcctgc tctcggccgt gctcgggctg ctcgtatggt tgtccgtcgc attgagcatg
     4561 atgttcctcg gacttccttt cggtgcattg catctgttgc ttcgctggat gggccactga
     4621 gcgatttcta cgcaaagggc actgaaaccc ggcttcacgt gttcgagatc cacacgctaa
     4681 tctccacaca tgactcacgc cgtcgctcaa cacttctgct gcacgctgga ttctccagtc
     4741 ggtgccctct cgctcgtcgc cacagaccag gcgctcgtcg ccgtcgtgtg gcgaagggaa
     4801 ggccacaggc acgtgccgtt cgagaatgca gtcgaagacc cgaaccattc gctgctgcgc
     4861 gagacgcagc gtcagttgcg cgaatacttc gcgagcaagc gccgcgcttt cgatcttccg
     4921 ctcgagtttc gaggaacaga tttccagcgc cgcgcgtggg ctgcgctgct caccattccc
     4981 tacggcgaaa cgcgcactta ccggcagatg gccgaacaga tcggcaatcc caccgcggtt
     5041 cgcgccgtcg gcgcggcgaa cggaagaaac cccatttcga tcatcgcgcc gtgtcatcgg
     5101 gtcatcggca tgaacggcga tctcaccggc tttggtggcg gcctcgatgc caaggcgcac
     5161 cttctctcgc tcgaagcgcc gcagcgccaa ttactcgcgg gctgaggccg gccggcgagc
     5221 gtcaatggct cgcgatcgac tcgtactggc gctgaagaaa acgtctctcg cgttcattgc
     5281 cgcactgttc gatggccgcc tggtacgcca ccgccgcgtc ggcgtcccgt ccgcagcggc
     5341 gcagcagatc ggcgcgcacc gcatgcagga ggtgatagcg gggcaggtcg agcgcatcca
     5401 ccagcaccag gccgcgttcc ggtccttcga gttcggccac gaccacggca cgattcagcg
     5461 cgatcaccgc gctcggcgcc agtgcgagca agtgatcgta gagcccgaga atctgttgcc
     5521 agtccgtatc cgcggcgctg cgcgcatcgc tgtgcaccgc gttgatcgcc gcctgcagct
     5581 gatagggacc gggccggttg cgcgcgaggc acacgcgcaa cagggtctgt ccttcctcga
     5641 tgcgcgtgcg gttccacgcg cggcgatcct gctcggccag ccgcacgaat tcgccggcgc
     5701 cgtcgacgcg ggcgtgtcgc cgtgcatcga tgagcaacat cagcgcaagc aagccgatcg
     5761 cttcggattc ctcgggcagc agctcgacca gcagccggcc gagacgaagc gcctcgtcgc
     5821 agagctcggc gcgaaccagc gcgtcacccg acgtggcgct gtagccttcg ttgaaaatca
     5881 ggtagacgac ggcgagcacc gcgccgaggc gcgccggcaa ttcgctgttg tccggaaccc
     5941 gatacggaat gcccgcatcg cgaatcttgg ccttggcgcg cgacagccgt tgcgccatgg
     6001 tcggctcggg gaccaggaac gcgcgcgcga tctccgcggt cgagaggcca cctaacaggc
     6061 gcagcgtcag cgccagctgc gcttcgggcg cgatcgccgg gtggcaacac gtgaagatca
     6121 ggcgcagccg gtcgtcgcgc acgtcgtgct cctccacggc ttcctgttcc agcagcagca
     6181 tcgcggcgtc ctggcgggca tcgcgcgtgg cttcgcgacg cagatgatcg agcgcgcggt
     6241 tgcgcgccgt ggtgatgatc cagccagccg gtgatgccgg tatgcccgtg aggggccagt
     6301 gcgtgatcgc cgtggtgaat gcgtcctgca ccgattcctc ggcgagcccg atgtcgccca
     6361 ggaaacgcac gaggacggcg accgcacggc cgtactcgcg gcggaagacc gctgcgacct
     6421 cgtcggtcag tgcccctccg cgaacggccg gacttcgatc ggcgtgccga tggcgcgcgc
     6481 gagcttgcgg ccccaggcca gcgcctcgtc gagatcgggc gccttgagga tggtgatgcc
     6541 gccgatctgt tccttgctct cgatgaaggg tccatcggtg gtgatgagcc cgccgccctg
     6601 gtggcgcacc acggtggctg aatccggtgg tgtaagcccg ccgccgaaga tcca
//
LOCUS       DATDEU010000199         4674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_764834, whole genome shotgun sequence.
ACCESSION   DATDEU010000199 DATDEU010000000
VERSION     DATDEU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764834"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3520)
                     /locus_tag="VIV63_04870"
     CDS             complement(<1..3520)
                     /locus_tag="VIV63_04870"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6123962.1"
                     /translation="MPLPQFHPAVQAWFASTFPNGATPAQLDAWPAIASGNHALIAAP
                     TGSGKTLAAFLAAIDSLAREGAEGRLRDVTRVVYVSPLKALSNDIQRNLEQPLAGVSA
                     EMLKLGSRAPEIRAAVRTGDTTQTERAAMRRTPPHILVTTPESLYLLLTSASGREMLT
                     TVRTVIVDEIHAVAQSKRGAHLTLTLARLSANAGQPVQRIGLSATQKPLEEVAKFLVG
                     EAVPRGKGDRSIFPQGDLPFDQPPEKIDLSPFSTRIVNSGHVRERDINLVMPSAPLEA
                     VMSGEVWATVYDQIAKLIEEHHTTLVFANTRRMVERVSRHLAERIGEENVAAHHGSLS
                     KETRFDAEQRLKAGKLKVMVATASLELGIDIGDVELVCQLGTPRSISVFLQRVGRANH
                     SVGGVPKGRIFPGSRDELIDCVALLDSVRRGELDRLRIPEHPLDVLSQQIVAEVSARE
                     YGEDELFTLVRSAHPYRNLDRKDFNAVVRMLAEGIDTKRGRRGTYLHRDAVNQVLRAR
                     KGARLTAITCGGAIPDNADYQVILEPAGIFVGTLNEDFAIESLAGDVFQLGNTSYRIL
                     RVEAGRVRVEDAKGQPPSIPFWLGEAPARTDELSESVSRLRADIEALLPEVTAESLAR
                     AIDIVIERYHLPRPAAHQMVEYLAAAKAVLGQLPKRDTLVFERFFDESGGQQLIIHAP
                     FGARVNRAFGLSLRKKFCRTFNFELQAAATEDAIVLSLGETHSFDLESVARFLNSKTV
                     EDTLIQAMLDSPMFTARWRWNASISLAIRRNMGGKRTPPQIQRMASEDLIAVLFPDQI
                     ACAENLSGPREIPDHPLVKQTLQDCLHDAMDIDGLRNLLIGLETGTIKVVAKDLIEPS
                     PLAAEILTARPYAYLDDAPLEERRTLAVSQRRWLDPESAADIGKLDPAAIAAVREQAW
                     PEVTNPDELHDAMHATGVITEQEGASAGWTRHLDALIDARRATRLHTASHVFWVCAEC
                     LPLVQAVYPEGRREPQVEPPAEKAEKIWQREEAITDLVRGRLQSVGPITARDMAATLG
                     LALSEINIAFIELESEGFVLRGRFTPEASTTEAMQTGDETLEWCERRLLARIHRYTIK
                     TLRAEIEPVSGADFMRFLLDWQGVTRQPKPEGVESLAAVITQLEGYEIPAAAWESDVL
                     AARLNDYDPHWLDSLCLSGRALWARLT"
     gene            complement(3648..4112)
                     /locus_tag="VIV63_04875"
     CDS             complement(3648..4112)
                     /locus_tag="VIV63_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY6123963.1"
                     /translation="MKDTTVNASMLIQRPPGEVFAAFVNPQQLRKFWLSDASGPLAPR
                     ARVVWTFMVPGATAKVAVTHFESPHHLAFDWDDGLHVDMTFEVFGEGGTRVGVGVSGF
                     DGADTLSQATDTIEGFAIVLCDLKTLLETGESAGLVRAKADLIIAAQAKQGG"
     gene            complement(4180..>4674)
                     /locus_tag="VIV63_04880"
     CDS             complement(4180..>4674)
                     /locus_tag="VIV63_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123964.1"
                     /translation="MQNWRDQVQIGMPGYRDRIVHVCHSDSEGGMNLNMDPKVIEDLA
                     EGGAGAAGLLRKAFVPGDDPLAGGWYNHRRIRMRTLLAGIDQKLRRINIVLGRPGSPG
                     WVDVVSDADSDAYQFKSEKHRALAKEVLQDLAQLGRKLDESGIDLADGVPRPESEWRP
                     TPRV"
BASE COUNT          768 a         1506 c         1543 g          857 t
ORIGIN      
        1 gggtcaaccg tgcccacaac gcgcgccccg aaaggcacag gctgtccagc cagtgcggat
       61 cgtaatcatt gaggcgcgcg gcgagcacgt cgctttccca cgccgcggcc gggatctcgt
      121 agccctcgag ctgcgtgatc acggcggcga ggctctcgac gccttcgggt ttgggttgtc
      181 gcgtgacgcc ctgccagtcg agcaggaagc gcatgaaatc cgcgcccgac acgggttcga
      241 tttccgcgcg cagcgtcttg atggtgtagc ggtggatgcg cgccagcaat cgccgctcgc
      301 accactccag cgtctcgtcg ccggtctgca tcgcctcggt cgtgctggct tcgggcgtga
      361 agcggccacg cagcacgaat ccttcgcttt cgagttcgat gaacgcgatg ttgatttccg
      421 agagagcgag cccgagggtc gcggccatat cgcgagccgt gattggcccc acgctttgca
      481 gccggccacg cacgagatcg gtgatggcct cctcgcgctg ccagattttt tccgccttct
      541 ccgccggcgg ctcgacctgc ggttcgcgcc ggccttcggg gtacaccgcc tgcacgagcg
      601 gcagacattc agcgcacacc cagaagacgt gtgatgcggt gtgcaggcgc gtcgcgcggc
      661 gtgcatcgat gagcgcatcg aggtgccgcg tccagcccgc actcgcacct tcctgctcgg
      721 tgatcacacc cgtcgcgtgc atggcatcgt gcagctcatc gggattcgtg acctcgggcc
      781 acgcctgttc gcgcacggcg gcgatggcgg ccggatcgag cttgccgatg tccgcggcgc
      841 tttccgggtc gagccaacgg cgctgagaaa ccgcgagcgt gcggcgctct tcgaggggcg
      901 cgtcgtccag ataggcgtag ggacgcgcgg tgaggatttc agcggcgagt ggtgacggct
      961 cgatgagatc tttggcgacg accttgatgg tgccggtctc gaggccaatg agcagattgc
     1021 gcagcccgtc gatgtccatg gcgtcatgca ggcagtcctg cagcgtctgc ttcacgagcg
     1081 ggtgatccgg gatctcgcgc ggccctgaga gattctccgc acaggcgatc tggtccggga
     1141 acaacaccgc gatgagatct tcggaagcca tgcgctggat ctgcggtggc gttcgcttgc
     1201 cacccatgtt gcggcggatc gcgagcgaga tgctcgcgtt ccagcgccag cgcgcggtga
     1261 acatcggcga atcgagcatg gcctggatca atgtgtcttc gacagtcttg ctgttgagga
     1321 agcgcgccac gctttccaga tcgaagctgt gggtctcgcc cagcgacagc acgatggcgt
     1381 cttccgtggc agcggcctgc agttcgaagt tgaacgtgcg gcagaatttc ttgcgcaatg
     1441 acaggccgaa cgcgcgattc acgcgcgcgc cgaagggcgc atggatgatg agctgctgcc
     1501 cgcccgattc gtcgaagaac cgctcgaaca ccagcgtgtc gcgtttcggg agctggccga
     1561 gcacggcttt cgccgccgcg aggtattcga ccatctgatg cgcggccgga cgcggcaaat
     1621 gataacgctc gatcacgatg tcgatcgcgc gcgcgagaga ttccgccgtc acttccggca
     1681 gcagcgcttc gatatccgcg cgcagccgcg acacggattc cgacagttcg tcggtgcgcg
     1741 ccggcgcctc gcctaaccag aacgggatgc tgggcggctg gcccttcgcg tcttcgacgc
     1801 gcacgcggcc tgcttcgacg cgcaggatgc ggtacgaggt attgccgagc tggaagacat
     1861 cacccgcgag gctctcgatg gcgaagtctt cgttgagcgt gccgacgaag atgcccgcgg
     1921 gctcgaggat cacctggtag tcggcgttgt cggggatggc gccgccacag gtgatcgccg
     1981 tcagccgcgc gcccttgcgc gcgcgcagca cctggttgac ggcgtcacga tgtagatagg
     2041 tgccccgccg gccgcgcttg gtgtcgatgc cttcggcgag catgcgcacc acggcgttga
     2101 agtccttgcg gtcgagattg cgatacggat gcgcgctgcg cacgagcgtg aagagttcgt
     2161 cctcgccgta ttcgcgcgcc gaaacctcgg cgacgatctg ctgcgacaac acgtcgagcg
     2221 ggtgttcggg gatacgcagg cggtcgagct cgccgcggcg cacggagtcg agcagcgcga
     2281 cgcagtcgat gagttcgtcg cggctgcccg ggaaaatgcg acccttcggc acgccaccca
     2341 cggagtgatt ggcgcggccg acgcgttgca ggaaaaccga gatgctgcgc ggcgtgccga
     2401 gctggcacac caactccacg tcgccgatgt cgatgccgag ctcgagcgac gccgtggcca
     2461 ccatgacctt gagtttgccc gccttgagcc gctgttcggc gtcgaagcgc gtttccttgg
     2521 aaagactgcc gtggtgagcc gcgacgtttt cttcgccgat gcgttcggcc agatggcgcg
     2581 agacgcgctc gaccatgcgg cgcgtgttcg cgaacaccag cgtcgtgtga tgttcttcga
     2641 tgagcttcgc gatctggtcg taaaccgtgg cccacacctc gcccgacatc actgcttcga
     2701 gcggcgccga cggcatcacc aggttgatgt cgcgttcgcg gacgtgcccc gagttgacga
     2761 tgcgggtcga aaaaggggac agatctattt tttctggtgg ctggtcaaat ggcaggtcac
     2821 cctgcggaaa aatagatctg tccccttttc cgcgtggtac cgcttcgccc accaggaact
     2881 tcgccacctc ttcgaggggc ttctgcgtcg ctgacaaacc gatgcgttgc accggctgcc
     2941 cggcattggc gctgaggcgc gcgagcgtca gcgtcaggtg ggcgccacgc ttgctctgcg
     3001 cgacggcatg gatttcgtcg acgatgaccg tgcgcaccgt cgtgagcatc tcgcgccccg
     3061 aagcactggt cagcagtaga tagagcgatt cgggcgtggt gacgaggatg tgcggcggcg
     3121 ttcgccgcat cgccgcgcgt tcggtctgcg tggtgtcacc cgtacgcacc gccgcgcgga
     3181 tttccggcgc acgcgacccc agcttcagca tctccgccga cacgcccgcc aggggctgtt
     3241 cgagatttcg ctggatgtcg ttcgagagcg ccttcagcgg cgacacgtac acgacgcgag
     3301 tgacgtcgcg cagccgccct tccgcgcctt cacgcgccag cgaatcgatg gccgcgagaa
     3361 aagccgccag cgtcttgccc gagcccgtgg gcgccgcgat cagtgcgtgg ttgccgctgg
     3421 cgatcgccgg ccaggcgtcg agctgcgctg gcgtcgcacc attcggaaaa gtgctggcga
     3481 accacgcctg cacggcggga tggaactgcg ggagtggcat ggatgggact ctatatagag
     3541 gcgtcaggcc gttccatcca gcctctccgc gttctcaccc ctgtatatcg cccacgcact
     3601 taggaaatcg agcgaaatcg cgcagatacg cgaagagccg gcgcgcttca gccgccctgc
     3661 ttcgcctgcg ccgcgatgat gaggtcggcc ttggcgcgga ccaggcccgc ggactcgccg
     3721 gtctcgagca aagtcttgag atcgcacagc acgatggcaa aaccttcaat cgtgtcggtg
     3781 gcctggctca gcgtatccgc accgtcgaag ccgctgactc ccacaccgac gcgcgtgccg
     3841 ccttcaccaa aaacctcgaa cgtcatgtcg acgtgcagtc cgtcatccca gtcgaacgcg
     3901 aggtggtgtg gtgattcgaa atgcgttacg gccactttcg cagtggcgcc cgggaccatg
     3961 aaagtccaga ccacacgcgc gcgtggcgcg agcggaccac tggcgtcgct caaccagaac
     4021 ttgcgaagct gctgcggatt gacgaaggcg gcgaacacct ctccgggtgg ccgctgaatc
     4081 aacatgctgg cgttgacggt cgtgtctttc atgccgtcat ttgaacgcgg gaaaacctcg
     4141 gtgaaagtag gcgcacgccg ggtctgctgg ttcaaacctt catacgcgcg gcgtgggccg
     4201 ccactccgat tccggccgcg gcaccccatc ggctagatcg atcccgctct cgtcgagctt
     4261 gcggcccagt tgcgcgagat cttgcaggac ctctttcgcg agggcccgat gtttctcgct
     4321 cttgaactgg taggcgtcgc tgtcggcatc cgagacgacg tcgacccagc caggtgagcc
     4381 cgggcggccg agcacgatgt tgatcctgcg caacttctgg tcgatgcccg cgagcagcgt
     4441 ccgcatgcgg atgcgacggt ggttgtacca gccgcccgcg agaggatcgt cgccgggaac
     4501 aaacgccttg cgcagcaggc cggcggcgcc agctccgccc tcggcgagat cttcgatgac
     4561 cttcggatcc atgttgagat tcatgccgcc ttcgctgtcc gagtggcaga cgtgcacgat
     4621 tcgatcgcga tagccgggca tgccgatctg cacctggtcg cgccagttct gcat
//
LOCUS       DATDEU010000200         7784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_618853, whole genome shotgun sequence.
ACCESSION   DATDEU010000200 DATDEU010000000
VERSION     DATDEU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618853"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VIV63_04885"
     CDS             complement(<1..853)
                     /locus_tag="VIV63_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6123965.1"
                     /translation="MTSARAAHSSLALTLRRIDLDRGERAVLRDVNWRIQPGQRWLLI
                     GGNGAGKTQLLKLLAGSVWPKPTGKELRRYRWRGENFDTPAGVSEEIAYVGAERQDRY
                     EHYEWNFRARDVVGTGIQRTDIPMRELTAAESKQVGALLQRLGISALADRRFLTLSYG
                     ERRLVLIARALASRPRLLLLDEVANGLDARNHAQFLTWLNRTTRSSLPWVFATHRLPD
                     VPDSMTHLLELEQGTVKRSGAMRPDKARELLQQDARAFLSRKTHTRVRSRSRRSLVTL
                     RHADVHVD"
     gene            complement(898..1761)
                     /gene="phhA"
                     /locus_tag="VIV63_04890"
     CDS             complement(898..1761)
                     /gene="phhA"
                     /locus_tag="VIV63_04890"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEY6123966.1"
                     /translation="MARLPAAPTQAPVKLRGDYAHAAADFRLAQKYDDYTADEQAIWR
                     TLYERQLGMLGEHAAPEFMAGLRLLGADAHRIPVLEEASERLKRLTGFEVIGVPGLIP
                     EADFFAHLAERRFPVTVWMRRRDELDYLVEPDLFHDFFGHLPMLAHTVFANFIQAYGA
                     IGAHARSPAALKMLARLYWYTVEFGLVRTSRGLRAYGAGILSSSGETIYATRATGPQR
                     IAFDLNRVLRTNYLIDSYQKTYFVLDDIEQLFDSVLAADFETLFAHADEPAYEPDARL
                     PGDQPTSLNLS"
     gene            1876..2352
                     /locus_tag="VIV63_04895"
     CDS             1876..2352
                     /locus_tag="VIV63_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY6123967.1"
                     /translation="MKLDKTDRRILDVLQRDGRISVTDLAERVGLSATPCARRIKQLE
                     ESGLIQGYAAIVDPKRAGQTIQAIVQIKLEQHSDEIVERFRRTLIDRPEVLACYTMTG
                     EMDFLLHVIVRDIEALADFTMRRLLRVSGVRDVTSSIVLETVKRSPVIPLEELAPE"
     gene            complement(2583..2659)
                     /locus_tag="VIV63_04900"
     tRNA            complement(2583..2659)
                     /locus_tag="VIV63_04900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2623..2625),aa:Arg,seq:tcg)
     gene            complement(2696..4681)
                     /locus_tag="VIV63_04905"
     CDS             complement(2696..4681)
                     /locus_tag="VIV63_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6123968.1"
                     /translation="MTLRKPVRALILLSAVGSTVAFAADPASTIDTIVISASPITPED
                     KLATIVETVDRAEILRAGGANLADALANTPGVTGSGFASGASRPVIRGFDSSRVRTLE
                     GGIGSFDVADVGPDHGIPIDPLSAQRIEVIRGAATLRYGSQAIGGVINAINNRVPARL
                     PDDPFAAELTGMYGTNADTRQGSALVDGRIGSFALHADGFKRETDDYDIPGGTMENSF
                     FRGDGYSLGGSYLFDKNRIGAAVIRYDSEYGIPGEGAYIDMKQTKELLRTSFAPQIGA
                     FHTLTVEGGHADYEHSEIELDTGEVGSTFTDDEWDARAEALFGVIGPFSAAALGVQFQ
                     NREFAALGEGADYLLPTETRTAAAFAFADAPLSAQLHLHLGARVESVDVAGTPLDDVA
                     TSRSFTPISASAGLLFDASDALQLGFTLSSAARAPGQTELYARGPHEATATFETGNAG
                     FDVERANSLEATLRFDTGRASFEAAVWGSDFTDYIYGNLTGRTCDDDGVCVDDDSGEL
                     DELIYEQRDARYWGAEARATVEVAEVGGVPLRGIVLADYVRATLDAGGEVPRISPYHL
                     GAGLDWNGALFGGGFTFKYTGPREHNLAVAETPTPGFVSLDAQATWRPFGQNPDVQFS
                     LVGRNLTDRTQRNAISLNKDEVVMPGRDVRLMVWAKF"
     gene            complement(4745..5026)
                     /locus_tag="VIV63_04910"
     CDS             complement(4745..5026)
                     /locus_tag="VIV63_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123969.1"
                     /translation="MHVLALAFLFAQLGMVAHASSHLNSDLHAKPTAGQLCGECASFS
                     PVQNMVGGAPIVVLAVQVSHDRAAECAAVVSLPHRAFSAFRSRAPPIPS"
     gene            complement(5177..6889)
                     /gene="araD"
                     /locus_tag="VIV63_04915"
     CDS             complement(5177..6889)
                     /gene="araD"
                     /locus_tag="VIV63_04915"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEY6123970.1"
                     /translation="MKRRLRSQDWFGKQDKDGFIHRSWLKNQGYPDDMFDGRPVIGIC
                     NTWSELTPCNGHFRELAEFVKRGVLEAGGFPLEFPVMSLGETQMKPTAMLFRNLASMD
                     VEESIRGNPMDGVVLLMGCDKTTPSLLMGAASCDLPTIGISGGPMLSGRFRGKQIGSG
                     TGVWQMSEDVRAGKMSQEEFTAAESCMHRSKGHCMTMGTASTMASMVEALGVSLPDNA
                     AIPAVDSRRYRLAQLSGRRIVEMVKEDLILSKILTRQAFENAIKANAAIGGSTNSVVH
                     LLAIAGRLGVKLELEDWDKHGSTLPCLVNLQPSGQYLMEDFYYAGGLPAVLKEIEGVL
                     NKDALTANGKTIGENIASAPVWNRDVIKAFDDPFKANAGIAILRGNLAPDGAVIKPSA
                     ATPKLLKHRGKAVVFETIEELKARIDDENLDIDENSIMVLKGCGPKGYPGMAEVGNMP
                     LPPKVLRKGITDMVRISDARMSGTAYGTVVLHVAPEAAAGGPLALVQTGDIIELDVPA
                     RKLHLDVSDTELARRRAAWKAPEPPKRGYYKLYVEHVQQADKGADLDFLVGGSGTTVT
                     RDSH"
     gene            6991..7767
                     /locus_tag="VIV63_04920"
     CDS             6991..7767
                     /locus_tag="VIV63_04920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6123971.1"
                     /translation="MPADRQDATYPSLRGRSVFITGGGSGIGGCLTEAFARQGSRVAF
                     VDYAEAPSLELVSRLDKAGLVKPWYRKTDVTDVSALQAAIRDAAQALGDFHVLINNVA
                     SDDRHSLMDVTPEYYDARIAVNQRPAFFAIQAVVPGMKKMGGGSIINFGSNSYGAKGR
                     NMPVYTTAKSSVIGLTRGLAAELGESRIRINVITPGWIMTERQIEKWLTPEGELEIKR
                     NQVLPDKVRPEDVAPMALFLASDDSRACSAQEYIVDAGWL"
BASE COUNT         1407 a         2497 c         2491 g         1389 t
ORIGIN      
        1 cgtccacgtg aacgtcggca tggcgcagcg tgaccagcga tcgccggctg cgcgaccgca
       61 cgcgcgtatg ggtctttcga gacaggaaag ccctggcgtc ctgctgcaac agctcgcggg
      121 ccttgtccgg ccgcatcgcg ccgctgcgct tcaccgtgcc ctgctcgagc tcgagcaggt
      181 gagtcatcga atccggcacg tccggcaggc gatgggtggc gaacacccac ggcagggatg
      241 aacgcgtcgt gcgattcagc caggtgagga actgcgcgtg gttacgcgca tcgaggccat
      301 tggcgacctc atcgagcaac aacaacctgg gccggctggc gagcgcgcgg gcaatgagca
      361 ccagccgccg ctcgccataa gaaagagtga ggaaacggcg atccgcgagc gcgctgatcc
      421 ccagacgttg cagcaaggcg ccgacttgtt tgctctcggc agccgtgagc tcgcgcatcg
      481 gaatgtccgt gcgctgaatg ccggtgccca cgacatcgcg cgcgcgaaag ttccactcgt
      541 agtgctcgta gcgatcctgg cgttccgcgc cgacgtacgc gatttcttcg ctcacacctg
      601 ccggcgtgtc aaagttttcg ccgcgccatc gataacgccg caactccttg cccgtcggct
      661 tgggccacac gctgcccgcg agcaacttga gcaattgcgt cttgccggcg ccgttaccgc
      721 cgatcaacag ccaccgctgg cccggctgga tgcgccagtt cacgtcgcgc agcaccgcgc
      781 gctcgccgcg atcgaggtcg atgcgccgaa gcgtcagggc cagggaagaa tgtgctgcgc
      841 gcgcgctcgt catcggcgcg ggagcttacc ggcgaaagcc cgccggcgcg aggcgcgtca
      901 ggaaaggttc aacgaggtcg gctggtcgcc gggcaggcgc gcatcgggtt cgtaggccgg
      961 ttcgtcggca tgcgcgaata acgtctcgaa gtccgccgcc agcacgctgt cgaacaactg
     1021 ttcgatgtcg tcgagcacga agtacgtctt ctggtacgaa tcgatgaggt agttggtgcg
     1081 caggacgcga ttcagatcga aggcgatgcg ctgcgggccg gtggcgcggg tcgcgtagat
     1141 cgtctcgccg ctcgacgaca ggatgccggc gccatacgcg cgcaagccac gcgaggtgcg
     1201 taccagtccg aactccacgg tgtaccagta caggcgcgcc agcatcttga gcgcggcggg
     1261 cgaacgcgca tgcgcgccga tcgcgccata cgcctggatg aagttcgcga acacggtgtg
     1321 cgcgagcatc gggagatggc caaagaagtc gtggaagaga tccggctcga ccaggtagtc
     1381 gagttcgtcg cggcggcgca tccacaccgt tacggggaaa cgcctttcgg cgagatgagc
     1441 gaagaagtcc gcctcgggga tgagcccggg cacgccgatc acttcgaaac ccgtcaggcg
     1501 cttcaggcgc tcgctcgcct cttccagcac ggggatgcgg tgggcgtcgg cgcctaacaa
     1561 gcgcaggccg gccatgaact cgggcgcggc atgttcaccc agcatgccga gctgacgctc
     1621 atacagcgtg cgccagatgg cctgttcgtc ggcggtgtag tcgtcgtatt tctgggcgag
     1681 ccggaaatcg gcggcggcgt gggcgtaatc gccacgcagt ttcacgggag cctgggtagg
     1741 agcagctggt aatcgggcca tgcggcatct tcctccgccc gctttgcaga aaaatggcaa
     1801 ttttcgtcag gaaatacctg aaaaagtggc gtcattctct atttttttga ctgattgtgg
     1861 ttaaatccgc cacttatgaa gctcgacaag acggataggc gcattctcga tgttcttcaa
     1921 cgggatgggc ggatcagcgt caccgacctc gcggagcgcg tcggcctttc ggcgactccc
     1981 tgcgcgcgcc ggatcaaaca gctcgaagag tcgggactca tccagggtta cgcagccatc
     2041 gtcgacccga agcgcgcggg acagacgatc caggcgatcg tgcagatcaa gctcgaacaa
     2101 cattccgacg aaatcgtcga acgcttccgg cgcacgctga tcgaccggcc cgaggtcctc
     2161 gcctgctaca ccatgacggg cgagatggac ttcctgctgc acgtgatcgt gcgcgacatc
     2221 gaagcactcg ccgacttcac catgcgacgg ctgctgcggg tcagcggcgt tcgcgatgtc
     2281 acctcgtcca tcgtgctcga aaccgtcaag cgctcgccgg tcattccgct ggaggaactc
     2341 gcgcccgagt aagggagctc tgaaaacgaa ttggcgcgcc cggacggatt gttcgccgcg
     2401 gaccgcggcg aatccctcgc taacgctcgg gaccaaacgc gcaaagccgc gtttgtccaa
     2461 ctcggggtga ctggtgatac caggtcgtca ggcagccgcc gagttgtcga accaatccac
     2521 cacccacaag gtgttggccg atgagtccga aaatcgaccg gcagtccgct ctaaaaaaga
     2581 attggcgcgc ccggacggat tcgaaccgcc gaccacctgg ttcgaagcca ggtgctctat
     2641 ccagctgagc tacgggcgcg tcacgggcat tctagccgcg agcgcccgcg ccggttcaaa
     2701 actttgccca gaccatcagc cgcacgtcgc gcccgggcat caccacctcg tccttgttga
     2761 gcgaaatcgc attgcgttgc gttcgatcgg tcagattccg gcccaccagg gagaactgca
     2821 cgtcggggtt ctgcccgaac gggcgccacg tggcctgcgc atccagcgat acgaatccag
     2881 gtgtcggggt ttcggcaacg gccaggttgt gttcgcgggg accggtgtat ttgaacgtga
     2941 aaccgccccc aaacaatgcg ccattccagt cgagtccggc gcccaggtgg tatggcgaaa
     3001 tgcgcggaac ctcaccgcct gcatcgagcg tcgcgcgcac gtagtccgcg agcacgattc
     3061 cacgcagcgg cacgccgccc acttcggcga cctcgacggt cgcgcgcgcc tcggcgcccc
     3121 aatacctcgc atcgcgttgt tcgtagatga gctcatcgag ctctccgctg tcgtcgtcga
     3181 cgcaaactcc gtcgtcgtcg caggtgcgtc cagtcagatt tccgtagatg tagtccgtga
     3241 agtccgagcc ccagacggcc gcttcgaagc tggcgcgtcc ggtatcgaag cgcagcgtgg
     3301 cctcgagcga attcgcgcgc tccacgtcga agccggcatt gccggtttcg aaggtggccg
     3361 tcgcttcatg cggcccgcgc gcatacagct cggtctgccc cggcgcccgc gcggcgctcg
     3421 acagcgtgaa tccgagctgc aacgcatcgc tggcgtcgaa caacaatccc gccgatgcgc
     3481 tgatgggcgt gaagctgcgt gaagtcgcaa cgtcgtccag cggcgttccg gcgacgtcca
     3541 cggattccac gcgcgcgccc aagtgcaggt gcagctgcgc ggatagcggc gcatccgcga
     3601 atgcgaacgc tgccgccgtc ctggtctcgg tcggcagtag atagtccgcg ccttcgccca
     3661 gcgccgcgaa ctcgcgattc tggaactgca cgccgagcgc cgccgcggag aacggcccga
     3721 tgacaccgaa caacgcctcg gcgcgcgcgt cccattcgtc atcggtgaac gtcgaaccca
     3781 cctcgcccgt atcgagttcg atttcgctgt gttcgtagtc cgcgtggccg ccttcgaccg
     3841 tcagcgtgtg gaacgcgccg atctgcggtg caaacgacgt gcgcagcagt tccttcgtct
     3901 gcttcatgtc gatgtaggcg ccttcgcccg gaattccgta ttcggagtcg tagcggatga
     3961 ccgcggcccc gatccggttc ttgtcgaata gataggaacc gccgagggaa tagccatcgc
     4021 cgcgaaagaa cgaattctcc atcgtgccac cggggatgtc gtagtcatcc gtttcccgct
     4081 tgaacccgtc ggcatgaagg gcgaacgagc cgatgcgccc gtccacgagc gccgaaccct
     4141 ggcgcgtatc cgcgttcgtg ccgtacatcc cggtgagctc cgccgcgaac ggatcgtcgg
     4201 gcaaacgcgc cggcacgcgg ttgttgatcg cattgatgac accgccgatc gcctggctgc
     4261 cataacgcag cgtcgccgct ccgcgtatca cctcgatgcg ctgcgccgac aacggatcga
     4321 tcggaatgcc gtgatccggc ccgacatcgg ctacatcgaa gctgccgatg ccgccttcga
     4381 gtgtgcgcac ccggctcgaa tcgaagccgc gtatcaccgg tcggctggcg ccggacgcga
     4441 aaccactgcc ggtcacaccg ggagtattcg cgagcgcatc ggcgaggttg gcgccgccgg
     4501 ctcgcaggat ctccgcccgg tcgacggttt cgacgatggt ggcgagcttg tcctcgggcg
     4561 tgatcggcga ggcgctgatg acgatggtgt cgatggtgga cgcgggatcg gccgcaaagg
     4621 cgaccgtgct gccgacggcc gacagcagta tcagagcccg aacgggcttg cgtagggtca
     4681 tggaaatctc cttccgaaac taacaaacac gcggcgcaca gcgcggcgtg ggattacttg
     4741 tcgttcagga agggatcggt ggagcgcggg aacggaacgc cgagaaggcg cgatgcggaa
     4801 ggctgacgac cgcggcgcat tccgccgcgc gatcgtggga aacctggact gccagtacga
     4861 cgatgggcgc gccgcccacc atgttctgca ccggcgagaa ggaggcgcac tcgccgcaga
     4921 gctgtcccgc agtcggcttg gcgtgcagat ctgaattgag gtggctgctg gcgtgcgcca
     4981 ccatgccgag ctgggcgaac aggaacgcca gcgccagcac atgcacgaac cagttgcgat
     5041 tgcgtcggat cagatcagcc tccgcgggag agtattcccc cgatagacgc ccgccgacaa
     5101 gctgacccta cccattttta tggagccgcc ccgtgcttcc atggaggggc ggcgtttccg
     5161 atgtgggcgt cagccctcaa tgagagtcgc gcgtcaccgt ggtgccgctg ccgcccacca
     5221 ggaagtcgag atcggcgccc ttgtcggcct gctgcacgtg ctcgacataa agcttgtaat
     5281 aaccgcgctt gggtggctcc ggcgccttcc acgccgcgcg tcgcctggcg agttcggtat
     5341 cgctgacatc gaggtgcagc tttcgcgccg gaacatcgag ctcgatgatg tcaccggtct
     5401 gcaccagcgc gagcgggccg cccgcggccg cctcgggcgc cacgtgcagt acgaccgtgc
     5461 cgtacgcggt gccactcatg cgcgcgtcgg agatgcgcac catgtcggtg atgcccttgc
     5521 gcagcacctt cggtggcagc ggcatgttgc cgacttccgc catgccggga taacccttcg
     5581 ggccgcagcc cttgagcacc atgatggagt tctcgtcgat gtcgagattc tcgtcgtcga
     5641 tcctggcttt cagctcttcg atggtctcga acaccacggc cttgccgcgg tgcttgagta
     5701 gtttgggcgt cgcggccgac ggcttgatca ccgcgccgtc gggcgcgagg ttgccgcgca
     5761 ggatggcgat gccggcgttg gccttgaagg gatcgtcgaa ggccttgatg acgtcgcgat
     5821 tccacacggg agcgctggcg atattttcac caatcgtctt gccgttggcg gtcaacgcgt
     5881 ccttgttgag cacaccctcg atttccttga gcaccgcggg caacccgccg gcgtagtaaa
     5941 aatcttccat gaggtactgg cccgaaggct gcaggttgac gaggcacggc agcgtggagc
     6001 cgtgtttgtc ccagtcttcg agttcgagct tgacgcccag ccggcccgcg atggctaaca
     6061 agtgcaccac ggagttggtg gagccgccga tcgccgcgtt ggccttgatg gcgttctcga
     6121 acgcctggcg cgtgaggatc ttcgacagga tgaggtcttc tttcaccatc tcgacgatac
     6181 gccggccgga cagctgcgcg agccggtaac ggcgcgagtc gacggccgga atggcggcgt
     6241 tgtcgggtag tgagacgccc agcgcctcga ccatgctggc catcgtcgac gccgtaccca
     6301 tggtcatgca gtgacctttc gatcggtgca tgcaggactc ggcggcggtg aattcttcct
     6361 gcgacatctt gccggcgcgc acgtcttccg acatctgcca gacaccggtg cccgagccga
     6421 tctgtttgcc gcggaagcgg cctgacaaca tcgggccgcc ggagatgccg atcgtcggca
     6481 gatcgcaact cgccgcgccc atgagcaacg atggcgtggt cttgtcgcag cccatgagca
     6541 gcacgacgcc atccattgga ttgccgcgaa tcgattcttc gacatccatg ctcgcgagat
     6601 ttcggaacaa catcgcggtg ggcttcatct gcgtttcacc cagcgacatc accggaaact
     6661 cgagcggaaa accgccggct tcgagcacgc cacgtttgac gaattcggcg agttcgcgga
     6721 agtgcccgtt gcagggagtg agctcgctcc acgtgttgca gatgccgatg accggacggc
     6781 catcgaacat gtcgtccgga taaccctggt tcttgagcca gcttcgatgg atgaagccgt
     6841 ctttgtcctg tttgccgaac cagtcctgac tgcgcaaccg ccgcttcatc ttcggaaccc
     6901 gtgaatgaga aggggcgcac cttcgcacaa gcagcgccgg aatatctata cttcagcgcc
     6961 tttcgaacac cgcaatcgag gctcattcgc atgcccgccg atcgtcaaga cgccacctac
     7021 ccctccctgc gcgggcgcag tgttttcatc accggcggcg gctcgggaat tggcggatgt
     7081 ctcaccgagg cgttcgcgag acaaggatcg cgggtggcgt tcgtggacta tgccgaagcg
     7141 ccgtctctgg aactggtctc gcggctggac aaggccggtc tggtgaaacc ctggtaccgc
     7201 aaaaccgacg tcacggatgt cagcgcgctg caggccgcga tccgcgacgc cgcccaggcg
     7261 ctgggcgact tccacgtact catcaataac gtggccagcg acgaccgcca ttcactgatg
     7321 gacgtgacgc ccgagtatta cgacgcgcgc atcgcggtga atcagcgccc ggcgttcttc
     7381 gcgatccagg cggtggtgcc gggcatgaag aagatgggtg gcggctccat catcaatttc
     7441 ggcagcaact cttatggcgc caaggggcgc aacatgccgg tgtatacgac ggcgaaatcc
     7501 tcggtcatcg gcctgacgcg cgggctggct gccgaattgg gtgaatcacg cattcgcatc
     7561 aacgtcatca cgcccggctg gatcatgacc gagcgccaga tagagaagtg gctcacgccc
     7621 gagggcgagc tcgagatcaa gcgcaaccag gtgctgcccg acaaggtacg gccggaagac
     7681 gtggcgccca tggcattgtt cctcgcttcc gacgattcgc gcgcatgctc ggcgcaggaa
     7741 tacatcgtcg atgcgggttg gttatgagac tgctgcaatt cacc
//
LOCUS       DATDEU010000201         6220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_35285, whole genome shotgun sequence.
ACCESSION   DATDEU010000201 DATDEU010000000
VERSION     DATDEU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35285"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VIV63_04925"
     CDS             <1..400
                     /locus_tag="VIV63_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123972.1"
                     /translation="PYRYPGIAPFGENEASWDAARVQAYLQLPVDWANKHGVPKNRLV
                     ATEFGCVRRWPDCARYLEDVLSAIEVDGVHWAFFSFRGEWDGFDYELGAGKLPWQYWE
                     AQEKGLPYELKRGPNEAFEPILRRLRGVSQ"
     gene            complement(348..1775)
                     /locus_tag="VIV63_04930"
     CDS             complement(348..1775)
                     /locus_tag="VIV63_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123973.1"
                     /translation="MNVGLDTSVFESPDFADALRVQYRRASNGAIELDPVFESIAPIL
                     AAGQPARDGDLHFAMRQLSQNPSRQPIDTIGLLVASSHVDDNRMFGLMFDQGLAAVPG
                     DINAAVPREGAAVFVDTIAGRRTPEDVANEVLFTAIHELGHAFNLSHAPQEPPTFMSR
                     SAVNDRAYDGSAFFFNQRQQAWLSSADSDFRVRPGESVFLDRDSRNSAEIPSASGDDD
                     LKLAASLHPAECTRFEPIHLDISLSIKGRADRRVPRMLDPSHSAFRIFIEDPAGRSRL
                     YKNSVHCCATPRRLRVSRSAPFRRDIVLTIEGGHYTFREPGVYRVRVEFDCSPDCTLR
                     SNVAEINILAKPDKSFATTAKMLAMPSATRLLQYRCLPNAKVVNAMSGLIDSWKGRWD
                     ERQSLIAYRWALSVLREGVKPVSPALILQARRYLERCRSLLPHREERVEELLASVARH
                     SRLSPSRLATATRREAGAVSARMPR"
     gene            complement(1808..2893)
                     /locus_tag="VIV63_04935"
     CDS             complement(1808..2893)
                     /locus_tag="VIV63_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123974.1"
                     /translation="MRLKISITMLVATFALAGCASWVSVEKVTNDNPKVKGLRYSLST
                     PYLLMTPKADGTVTYEWLHLPDMSKQYAITKHSMLSTSTLEITLEHGLLKKIVGKHDS
                     TAVATAALGQAGTILAADETAKKTAADAQKTAAKAETEKLKTAVATAQAAQRAADLDF
                     KKAQAKRDFLNDQKPVDDKALADAELEVKMAQIDLNAAEADLARANASLAATGSTNGG
                     ANTDDKAQQFLRPLLFRVVQKDGGAVDLQAVNNPAEFPDYSGIASAGGSAGGAAPAAF
                     DCQWDATKKVLVLSAAEEATIVKDRITLNLGAAALAVKATLDPDKAAKSWKITFDKKL
                     APGKYHMQFPYKVGGADKTGAVDFTVK"
     gene            complement(2896..3423)
                     /locus_tag="VIV63_04940"
     CDS             complement(2896..3423)
                     /locus_tag="VIV63_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123975.1"
                     /translation="MPLNYRYHALRLAAAALPLMLGACAGLTVEGVGNDDQTTDEKAR
                     GVRFYQEAPFLFIYPDGQGGTAAEIRWLPDTRQVMSAKPYALLASNETTMEFVGSALT
                     SMILVTDETAVVKASLEALGKVMLAANTAGEDKGDQTAPVPRLYRIIVNGDGGVVGLG
                     TSQTLDVHGMSFAGK"
     gene            complement(3556..4314)
                     /locus_tag="VIV63_04945"
     CDS             complement(3556..4314)
                     /locus_tag="VIV63_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6123976.1"
                     /translation="MTKTITTLAAVALGAASTVVAAGAGNNRTTVVLVHGAFVDGSGW
                     AGVHRILRDDGYQVIVVQNPTTSLADDVAVTRRAIAAAEGNVILVGHSYGGVVITEAG
                     SDAKVSGLVYVAAFAPDQGESVSSLNANAPPGAPALPILPPVDGYLLLDRTKFPAVFA
                     ADVAPDTARFMADSQVPWSVQALEGKISSPAWKTKPSWYLIPQDDRVIPPAAQRAMAG
                     RAHASIREISGSHAFYVANPNAVAALIKEAVSKL"
     gene            complement(4311..4739)
                     /locus_tag="VIV63_04950"
     CDS             complement(4311..4739)
                     /locus_tag="VIV63_04950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY6123977.1"
                     /translation="MRFSSVFISLPLALFAVIALGAPAVTPKGTGIRYAEIPAGAYSV
                     VAEVRARPGKERQLRDATLSLVAQVRAEPNNLLYFLHEDREAPGHFVFYEIFASKADF
                     EAHNATPHVQAWFAKLPELADGGVKVVRMEILGNRAGEGS"
     gene            complement(4761..6095)
                     /locus_tag="VIV63_04955"
     CDS             complement(4761..6095)
                     /locus_tag="VIV63_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEY6123978.1"
                     /translation="MLDSANPYLAGVHTPMNQELTIENLSVTGTIPAALEGRYLRMGP
                     NPMAPNPAKYHWFSGDGMIHGIRIENGRALWYRNRWIRSERVSKALGEPRVDGPRHAF
                     DTVNTNVVQFAGKTLGLVEAGSTPVEIGETLETLRYTDFAGTLDGSFAAHPHVDPLTD
                     EMHGICYDVSRRRSVRYVVVRPDGKVRREVDIPIRHGPMIHDCAFTQRFAIILDLPVT
                     FSLLSAITRYPFPFRWNSSHAARVGLLPREGGAGDIIWCPIDPCYVFHAVNSYDAADG
                     TVILDVIVHDRMFSRIKAGPDSQRVSFERWIVDPATRNVKRVVIDPTPQEFPRPDERR
                     LGQPYRYAYTMAMTGWFLGSGLYKHDLEKRTRQFHDFGPHRHPCEFMFVPAHPLAEED
                     EGWLFGFVIDASERTTDLVILDARNFEGPPQASIRVPHVVPPGFHGNWLQDA"
BASE COUNT         1135 a         1923 c         1968 g         1194 t
ORIGIN      
        1 tccctatcga tatccgggca tcgcgccatt tggcgagaac gaagcgagtt gggatgcagc
       61 gcgggtccaa gcctatctac aactgcccgt cgactgggcc aataaacacg gcgtgccgaa
      121 gaaccggctc gtggccaccg agttcggttg tgtgcggcgc tggccggatt gcgcgcggta
      181 ccttgaagac gtgctctccg cgatcgaagt tgacggcgtt cactgggcat ttttctcctt
      241 ccgcggcgaa tgggacggct tcgactacga actcggcgcg ggcaagttgc cctggcagta
      301 ctgggaggcg caggaaaaag gtcttccgta tgaattgaag cgagggccta acgaggcatt
      361 cgagccgata ctgcgccggc ttcgcggcgt gtcgcagtag cgagccgcga gggcgagagc
      421 cgggagtgcc gcgccaccga tgcgagcagt tcctccactc gttcctcccg gtgcggaagc
      481 aacgaccggc atcgctcgag ataccgccgg gcctggagga tgagcgcggg cgataccggc
      541 ttgacgcctt ctcgcaggac ggacagcgcc catcgatagg cgatcaggct ctggcgctca
      601 tcccaacggc ccttccagga atcgatgagg cctgacatcg cgttcacgac tttcgcgttc
      661 ggcagacaac ggtactggag cagccgcgtg gcgctcggca tcgccagcat ctttgcggta
      721 gtcgcgaatg acttgtcggg cttcgccaga atgtttatct ccgcgacatt cgaacgaagc
      781 gtgcagtcgg gcgagcaatc gaactcgaca cgcacgcgat agacgcccgg ctcacggaaa
      841 gtgtagtggc cgccttcgat cgtgaggacg atgtcgcggc ggaatggcgc ggatcgcgac
      901 acgcgaagtc gccgcggggt ggcgcagcag tgaacggaat tcttgtagag cctggaacgc
      961 ccggcgggat cctcgatgaa gatccggaag gcactgtgcg acgggtcgag catgcgcggc
     1021 acgcgccggt ccgcacggcc tttgatcgag agcgagatgt ccagatgaat cggctcgaac
     1081 ctcgtgcact ccgccggatg gagcgacgcc gcgagtttca gatcgtcatc gcccgaggcc
     1141 gatgggatct cggctgaatt gcgcgagtcg cgatcgagaa acacggactc gccgggccgc
     1201 acgcgaaaat cagagtccgc actggatagc caggcctgct gccgctgatt gaagaagaag
     1261 gcgctgccat cgtaggcgcg atcgttgacc gcgctgcgcg acatgaatgt cggcggctcc
     1321 tgcggcgcgt gactcagatt gaatgcatgt ccgagttcat gaatggcggt gaagaggacc
     1381 tcgttggcaa cgtcctccgg cgtgcggcgg ccggcaatgg tatcgacgaa caccgctgcg
     1441 ccttccctcg gtaccgcggc attgatatcc cctggaaccg cggcaagacc ctgatcgaac
     1501 atcaggccga acatccggtt gtcgtctacg tgtgatgagg cgactaacag accgatggtg
     1561 tcgatgggct gtctcgaggg gttctgcgaa agctgcctca tggcgaaatg caggtcaccg
     1621 tcgcgtgccg gctgcccggc ggcgaggatg ggtgcaatgc tctcgaatac gggatcgagt
     1681 tcgatggcgc cgttcgaagc gcggcggtat tgcacgcgca acgcgtccgc gaaatcaggc
     1741 gactcgaaga cactggtatc cagcccgaca ttcaaggccg cgattttgca ggtcagccgc
     1801 accggcatta cttcacggtg aaatcgaccg ctcccgtctt gtcagcaccg cccaccttgt
     1861 aggggaactg catgtggtac ttaccgggcg ccagcttctt gtcgaaggtg atcttccagg
     1921 acttggcagc tttgtccggg tcgagggtgg ctttcacggc gagtgcagcg gcgccaagat
     1981 tcagcgtgat ccggtctttg acgatcgtcg cctcttccgc cgcgctcagc accaggactt
     2041 tcttcgtggc atcccattgg cagtcgaatg ccgcgggcgc cgcacctccg gccgatccgc
     2101 ccgcagaagc aatacccgag tagtcaggga actcggccgg gttgtttaca gcctgcagat
     2161 caacagcacc accatctttc tgaactactc gaaagagcaa ggggcgcagg aactgctgtg
     2221 ccttgtcatc ggtgttcgcg ccaccattgg tcgagcccgt cgcggcgagc gacgcgttgg
     2281 cgcgcgcgag atctgcttcc gcggcgttca gatcgatctg cgccatcttc acttcgagtt
     2341 cagcgtcagc cagggccttg tcatcgacgg gcttctgatc attgaggaaa tcccgcttcg
     2401 cctgggcctt tttgaagtcg aggtcggccg cgcgctgtgc ggcttgcgcc gtggcgacgg
     2461 cggttttcag tttttcggtc tcagccttcg ccgccgtctt ctgggcgtcg gccgccgtct
     2521 ttttcgccgt ttcatccgcc gcgagaatgg tgcccgcctg ccccagcgcg gcggtggcga
     2581 ccgcagtgct gtcgtgcttg ccgactatct tcttgagaag accgtgttcg agcgtgattt
     2641 ccagggtgct cgtcgacaac atgctgtgct tggtgatggc gtactgcttg ctcatgtccg
     2701 gcagatgcag ccattcgtag gtgacggtgc cgtccgcctt cggcgtcatg agcaggtaag
     2761 gcgtcgacag tgaatagcgc aggcctttca ccttggggtt gtcgttggtg actttttcta
     2821 cgctcaccca gctcgcacaa ccggcgagcg cgaacgtggc aacgagcatc gtgatcgaaa
     2881 tcttgaggcg catgactatt tccccgcgaa tgacatgccg tgaacatcga gcgtctgcga
     2941 cgtgccgagt ccgacgacgc cgccatcgcc gttgacgatg atccggtaga gcctcgggac
     3001 tggtgccgtc tggtcgccct tgtcttctcc ggcggtgttc gccgcgagca tcaccttgcc
     3061 gagcgcctcg agcgacgcct tgaccacggc cgtctcgtcg gtgaccagga tcatcgacgt
     3121 gagcgcgctg ccaacgaact ccatcgtggt ttcattgctg gcaagcagcg catagggctt
     3181 tgccgacatc acctggcgag tgtccggcag ccacctgatc tccgcggcag tgccaccctg
     3241 accgtcggga tagatgaaca ggaatggcgc ttcctggtag aaacggaccc cgcgggcctt
     3301 ctcgtcggta gtttggtcgt cgttgccgac cccttccacg gtgagtccgg cgcaggcgcc
     3361 gagcatcaac ggaagtgcgg ccgccgcaag acgcagcgcg tgatatcggt agttcaatgg
     3421 catggttccg ctcccggccg acgcgcgggc attgaagcgc gtcacttatg actgccgtca
     3481 ggagcggatt gctttcacct cctcatggaa ttttttccgc ttctcccgga gcgaggccgc
     3541 cgcggccagc caccgtcata acttactgac cgcttctttg atcaatgccg cgacggcatt
     3601 gggattcgcg acataaaacg catggcttcc ggagatttcg cggatcgacg cgtgagcccg
     3661 acctgccatg gcgcgttgcg cggccggcgg aatgaccctg tcgtcctgcg ggatgaggta
     3721 ccaactcggc ttggtcttcc acgccgggct gctgatcttt ccctccaggg cctgaacgct
     3781 ccagggaacc tgcgaatccg ccatgaatcg cgcggtatcg ggcgccacat ccgccgcgaa
     3841 caccgccggg aacttcgtcc tgtcgagaag cagatagccg tcgactggtg gaaggatcgg
     3901 gagcgcggga gcgcccggtg gcgcgttggc gttcagcgaa gagacggact caccctggtc
     3961 gggcgcaaag gcagcgacgt aaacgagccc cgaaaccttc gcgtcagatc cggcctcggt
     4021 aatgacgacg ccaccgtacg aatgaccgac gagaatgacg tttccttccg cggccgcgat
     4081 cgctcgccgg gtaaccgcaa cgtcgtcggc cagcgaggtc gtcgggttct gaacgacgat
     4141 gacctgatag ccgtcatcgc gcaggatgcg gtggacaccg gcccagccgg acccgtcgac
     4201 gaacgcgcca tgtacgagca ctaccgtcgt gcggttgtta cctgcgcccg ccgcgacaac
     4261 cgtggacgcg gcgccgagcg ccacggccgc aagcgtagta atggtcttcg tcatgatcct
     4321 tctcccgcac gattgccgag gatttccatg cgcacgacct tgactccgcc gtcggcgagt
     4381 tcgggcagct tcgcgaacca ggcctgcaca tgcggagtcg cgttgtgcgc ttcgaaatcc
     4441 gccttgctgg cgaagatctc gtaaaacacg aagtgcccgg gcgcctcgcg atcctcgtgc
     4501 aggaaataga gcaggttgtt aggttcggcg cggacctgcg cgaccagcga gagcgtggcg
     4561 tcgcgcagtt gacgctcctt gcccggtctg gcgcgaactt cggcgacgac cgaatacgcg
     4621 cccgccggga tttcggcata acgaatgccc gtgccttttg gtgtcacggc tggcgcaccc
     4681 agggcgatga ccgcgaacag cgcgagcggc aaagatataa acaccgatga aaatcgcatt
     4741 gcatacctcc tgtttaactc tcaagcgtcc tgcaaccagt tgccatgaaa gcccggtggc
     4801 accacgtgcg ggacgcggat gctggcctgc ggtgggccct caaaattgcg cgcgtcgagg
     4861 atgacgaggt ccgtcgtccg ctcgctggca tcgatgacga agccgaagag ccagccctcg
     4921 tcttcctccg ccagcggatg agccggcacg aacatgaact cgcagggatg acgatgcggt
     4981 ccgaagtcat gaaactgtcg cgttcgtttc tccagatcgt gcttgtacaa acccgatccg
     5041 aggaaccatc ccgtcatggc catcgtgtag gcgtagcgat acggctgacc caggcgccgt
     5101 tcatccggac gcggaaattc ctgcggagtg ggatcgatca cgacacgctt cacgtttcgg
     5161 gttgcgggat ccacgatcca tcgttcgaaa ctgacgcgtt gcgaatcagg tccggccttg
     5221 atcctcgaga acatccggtc gtggacgatg acgtccagaa tcacggtgcc atccgccgcg
     5281 tcgtagctgt tgacggcgtg aaagacataa cagggatcga tcgggcacca gatgatgtcg
     5341 cccgccccgc cctcacgcgg caacagcccc acgcgggccg cgtgcgacga attccagcga
     5401 aacggaaatg ggtaacgagt gatcgccgac agcagcgaga acgtgacggg caagtcgaga
     5461 atgattgcga agcgctgcgt gaaagcgcag tcgtgaatca tgggcccgtg cctgatcggg
     5521 atatcgactt cgcgccggac ctttccgtcg ggcctgacta ccacgtaccg gaccgaacgc
     5581 cggcggctga cgtcatagca gatgccgtgc atctcgtccg tcaacggatc gacatgcggg
     5641 tgcgccgcaa agctgccatc cagcgttccg gcgaaatcgg tgtagcgcag agtctcgagc
     5701 gtttcgccaa tctcgacggg cgtgctcccc gcttcgacga gacccagcgt cttcccggcg
     5761 aactggacga cattggtgtt gaccgtgtcg aatgcgtgac gaggaccatc gacccgtggc
     5821 tctcccagag ccttgctcac cctctccgat cggatccatc gattgcggta ccagagtgcc
     5881 cggccgttct cgatccggat cccatggatc attccgtcgc cggagaacca gtgatatttg
     5941 gccggattgg gcgccatcgg attcgggccc atgcgcaggt agcggccctc gagtgcggcg
     6001 ggaatcgtgc cggtgacgga cagattctcg atcgtcagtt cctgattcat cggcgtgtgc
     6061 acgccggcca ggtagggatt cgcggagtcg agcatcgtga tctcctgact acttcgtgct
     6121 ggtcgctgtc agccgcttgc gagcctgcag tgcatcgtcg aaagtccgct tcacgggtcc
     6181 gagcgcctgc acgaactcga gctgttcgcc ctcgggcccc
//
LOCUS       DATDEU010000202         4752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_35471, whole genome shotgun sequence.
ACCESSION   DATDEU010000202 DATDEU010000000
VERSION     DATDEU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35471"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VIV63_04960"
     CDS             <1..598
                     /locus_tag="VIV63_04960"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY6123979.1"
                     /translation="LGSKANPGHRLFGVSGHVNRPGLYERPTGYPMEKLIFEDAGGIL
                     GGKPLKAVIPGGSSTPVLTAGEIEALTLDAPSLQQAGSMLGSGGVIVIAEGTCMVRLL
                     QVLTRFYRHESCGQCTPCREGTAWMDDIIARLISGRGEDADLANLQAIPAGILGNTLC
                     ALGDAAAMPVLSFAKKFRSEFEYFVENRRSLNGGRLEA"
     gene            607..2055
                     /locus_tag="VIV63_04965"
     CDS             607..2055
                     /locus_tag="VIV63_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6123980.1"
                     /translation="MELTIDDRTIEARSGESVLQAALRHGIEIPHFCYHPHLSIAGAC
                     RICLVKVEGVPKLMAACNLSVAPNMKVTTLVPEVGRAREHVMQFLMLNHPVDCGICDK
                     AGECRLQDYEYAYGLPRSRSTDAKHHKRKLHDLSPRIQLDNERCILCSRCVRFTREVS
                     KSNMLGIVERGAHAFVERADPTSADDPYSDNVIGLCPTGALLSRDFLYQSRVWFLEPV
                     RSVCTGCSRGCSIDLWRRKKEWRQRSLGEERNSMVYRVTAHENPEINGPWLCNKGFDQ
                     HKLMSRERTLTPMLGDAAASIDEALARARDLIAGARSPAALVSALASNEELDAFKRTL
                     GAHFRVYAREDSVPQPEEVVEDDFLIKSDKNPNSHGVRERFGWRALTPTDAQAHDLFF
                     IWGDWGDYESFGHARVIHLASFARQRDRHADVHIPLSTTFERSGTFSNFEGKQNRFEK
                     VLDKPPLVQHAEDVFAQLQAQLVGPQRHPEAP"
     gene            2052..3215
                     /locus_tag="VIV63_04970"
     CDS             2052..3215
                     /locus_tag="VIV63_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEY6123981.1"
                     /translation="MIPDLVVHGAFMLYAIMFMMLLATVFTWVERKQSAIMSDRIGAN
                     RCYIRIPFTNIKFVAWGLFHGIADGGKLLLKENFTPLTYDRLCYNLAPWLAAVPVLLV
                     FAVVPFGGVLVPSRLFDADTFPSLAAGLQGFFGERRYTMQVAGLDAGLLFVLAISGIG
                     VLGTMLAGWSSNNKFSLMGASRAASQMISYEVAMGLALISMVVTYGTLDLEAMVKWQS
                     GLLIGAVPAWGIFVQPLAFVLFLTASIAENKRIPFDLPEAESELVAGYFTEYTSMKMG
                     LFMLSEFIEIVVISALVVTLFLGGYNLPYLTDAGFAFPGGATWSLPHVAVVILQVLTF
                     AVKTFLVGCFQIQIRWTLPRFRYDQLMSLGWKVLLPLAAVNLVVTVVLRWVML"
     gene            3212..3781
                     /locus_tag="VIV63_04975"
     CDS             3212..3781
                     /locus_tag="VIV63_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY6123982.1"
                     /translation="MSERAAKKRYWNEPRMGLRERVYIIEVVRGLRITGGVFFGNMWK
                     WMTGRKGALTTYYPEERRSDYSPNNRGRHVLTQRPDGEVQCVSCNLCATACPAYCIEI
                     LSRANFADPAHPKAPERFEIDYSRCIFCGFCVEACPEDAIRMVKDVPDLPGFDRTAMW
                     GKMDLLMQWHPITNPKKPYPPAPPRADAT"
     gene            3814..4278
                     /locus_tag="VIV63_04980"
     CDS             3814..4278
                     /locus_tag="VIV63_04980"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY6123983.1"
                     /translation="MALIFALLVVILRQPMRAALALIAHMVSLAAIFACLEVHVVALF
                     QILIYVGAVMVFMVYAIMLLDDRDDSYVHRFSRWSVPAVIASLALIAALGAMLVQGAA
                     LAPPAAAVGPAPFSFSTFSVAFMTQYWFHFEVASVLLVIGIVAAWTVITERR"
     gene            4278..4580
                     /gene="nuoK"
                     /locus_tag="VIV63_04985"
     CDS             4278..4580
                     /gene="nuoK"
                     /locus_tag="VIV63_04985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY6123984.1"
                     /translation="MDRYEFLIALAVLMFATGLGGVVIRRNLLVVVMCVEVMLNSVVL
                     AFVAFAARTGTLAGPAMTFFIYIAASCEIALAMAIVVRLVERRGTLDLAADYELKG"
BASE COUNT          750 a         1617 c         1498 g          887 t
ORIGIN      
        1 gctcggcagc aaggcgaacc ccggccatcg cctgttcggc gtatccgggc acgtcaaccg
       61 gcccggactc tacgagcggc ccaccggcta tccgatggag aagctcatct tcgaggacgc
      121 cggcggcatc ctcggcggca agccgctcaa agctgtcatt cctggcggaa gttcgacgcc
      181 ggtgctcacg gccggcgaga tcgaggcact tacgctcgac gcgccgtcgc tgcagcaggc
      241 cggcagcatg ttgggatccg gcggcgtcat cgttatcgcc gaaggcacgt gcatggtgcg
      301 cctgctgcaa gtgctgacgc gcttctatcg ccatgaatcc tgcggccagt gcacgccctg
      361 ccgcgaaggg acggcctgga tggacgacat catcgcaagg ctcatcagcg ggcgcggcga
      421 ggacgccgac ctggcgaacc tgcaggcgat tcccgcaggc atcctcggca acacactgtg
      481 cgccctgggc gatgccgctg cgatgcccgt gctgagcttc gcgaagaagt ttcgcagcga
      541 gttcgagtac tttgtcgaaa accgccgctc gctcaacggc ggccgcctcg aagcctgagg
      601 gagcacatgg aactgacgat cgacgaccgc acgatcgaag cccgctcggg tgagagcgtg
      661 ctgcaggcgg cgctgcggca tggcatcgag atcccgcact tctgttatca cccgcacctg
      721 tcgatcgccg gcgcctgtcg tatctgtctg gtcaaggtcg aaggcgtccc gaagctgatg
      781 gccgcgtgca acctgtcggt cgccccgaac atgaaagtga cgacgctggt gccagaggtc
      841 ggccgcgcgc gcgagcatgt gatgcagttc ctcatgctca atcacccggt ggactgcggg
      901 atctgcgaca aggcaggcga atgccgcctg caggactacg aatatgccta cggtctgccg
      961 cgctcgcgct cgacggacgc caagcaccac aagcgcaagc tgcacgatct gagcccgcgc
     1021 atccagctcg acaacgagcg ctgcatcctc tgcagccgct gcgtgcgctt cacgcgagag
     1081 gtctcgaagt cgaacatgct cggcatcgtc gagcgcggcg cacacgcatt cgtcgagcgc
     1141 gccgacccga cctccgccga cgacccgtac tcggacaacg tcatcggcct gtgcccgacg
     1201 ggtgccttgt tgtcgcgcga cttcctctac cagagccgcg tgtggtttct cgaaccggtg
     1261 cgttcggtgt gcacgggctg ctcgcgcggc tgcagcatcg atctgtggcg gcgcaagaag
     1321 gaatggcggc agcgctcgct cggcgaggag cgcaacagca tggtgtaccg tgtcaccgcc
     1381 cacgagaacc ccgaaattaa cggcccctgg ctttgcaaca agggtttcga tcagcacaag
     1441 ctgatgagca gggagcggac gctgacgccc atgctcggcg acgccgcagc ctcgatcgac
     1501 gaggcgctcg cccgggctcg tgacctgatc gcgggggcgc gaagtccggc cgccctcgtc
     1561 tcggcgctcg cctccaacga ggaactcgac gccttcaaga ggacgctcgg cgcgcacttc
     1621 cgcgtctacg cgcgcgagga cagcgtccct cagcccgagg aggtggtcga ggacgatttc
     1681 ctgatcaaat ccgacaaaaa cccgaacagc catggcgtgc gagagcgctt cggctggagg
     1741 gcgcttactc ccactgatgc gcaggcccac gatctgttct tcatctgggg cgactggggt
     1801 gactacgaaa gcttcggcca cgcgcgcgtg atccacctcg cgtcgttcgc gcggcagcgc
     1861 gatcgccacg ccgacgtgca catcccgctg tccacgacgt tcgagcgcag cggaaccttc
     1921 tccaacttcg agggcaagca aaaccgcttc gaaaaggtac tcgacaagcc gccgctcgtg
     1981 cagcacgccg aggacgtgtt cgcgcagctc caggcgcaac tcgtcggccc gcaacggcat
     2041 ccggaggcgc cgtgattcct gacctcgtcg tccacggggc gttcatgctt tacgccatca
     2101 tgttcatgat gctgctggcg accgtcttca cgtgggtgga acgcaagcaa tcggcgatca
     2161 tgtccgaccg catcggcgcc aaccgctgct acatccgaat tccgttcacg aacatcaagt
     2221 tcgtcgcgtg gggcctgttc cacggcattg cggacggcgg caagctcctg ctcaaggaga
     2281 acttcacgcc gctgacctac gaccgcctct gctacaacct cgcgccctgg ctcgccgcgg
     2341 tgcccgtgct cctggtcttt gccgtcgtgc ccttcggcgg cgtactcgtg ccaagccggc
     2401 tcttcgatgc ggacactttt ccgtccctgg cggccggtct gcagggcttc ttcggcgaac
     2461 gccgctacac gatgcaggtg gccgggctcg acgccggcct gctgttcgtg ctcgcgatct
     2521 cggggatcgg cgtgctgggc acgatgctcg ccggctggtc ctcgaacaac aagttctcgc
     2581 tgatgggcgc ctcgcgcgcc gcctcgcaga tgatctccta cgaggtcgcg atgggactcg
     2641 cgctcatcag catggtcgtg acctacggca ccctcgatct cgaagcgatg gtgaagtggc
     2701 agtcggggct gctgatcggc gccgtgccgg catggggcat tttcgtgcag ccgctcgcct
     2761 tcgtcctctt cctcacggcg agcatcgcgg agaacaagcg catcccgttc gaccttcccg
     2821 aagccgagtc ggagctcgtc gccggctact tcaccgagta cacctcgatg aaaatgggcc
     2881 tgttcatgct gagcgagttc atcgagatcg tcgtcatctc cgcactcgtc gtgacgcttt
     2941 tcctcggcgg ctacaacctg ccgtatctga ccgacgcggg cttcgccttc cccggcggcg
     3001 ccacctggtc gcttccgcac gtcgccgtcg tgatcctcca ggtcctgacc ttcgcggtca
     3061 agaccttcct cgtcggctgc ttccagatcc agatccgctg gactctgccg cgcttccgct
     3121 acgaccagct gatgtcgctc ggctggaagg tcctgctccc gctcgcggcg gtgaacctcg
     3181 tcgtgaccgt cgtgctgcgc tgggtgatgc tgtgagcgaa cgagcggcaa agaagcgtta
     3241 ctggaacgag ccgcgcatgg gcctgcggga gagggtctac atcatcgagg tggtgcgggg
     3301 acttcgcatt accgggggcg tcttcttcgg caacatgtgg aagtggatga cgggtcgcaa
     3361 gggcgcgctg accacctact accccgagga gcggcgcagc gactactcac cgaacaatcg
     3421 ggggcgtcat gtcctgacgc aacgcccgga cggcgaagtc cagtgcgtgt catgcaacct
     3481 ctgcgcgacc gcctgccccg cctactgcat cgagatcctg tcgcgcgcga atttcgccga
     3541 tccggcgcat ccgaaggcgc ccgagcgctt cgaaatcgac tactcacgct gcatcttctg
     3601 cggcttctgc gtcgaggcct gcccagagga tgcgatccgc atggtcaagg acgtgcccga
     3661 cctgccgggc ttcgatcgca cggccatgtg gggaaagatg gacctgttaa tgcagtggca
     3721 tccgatcacg aatccgaaga agccctaccc gccagcgccg ccgcgcgccg acgctacatg
     3781 atcagcaacg tcctcatcgt cgccttcggc gccatggcgt tgatcttcgc gctgctcgtg
     3841 gtgatcctgc ggcagccgat gcgcgcggcg ctcgcgctca tcgcgcacat ggtcagtctg
     3901 gccgcgatct tcgcctgcct cgaggtgcac gtcgtcgcgc tgttccagat cctgatctat
     3961 gtcggcgcgg tgatggtgtt catggtctac gcgatcatgc tgctcgacga ccgcgacgac
     4021 tcctacgttc atcggttctc gcgttggtcc gtgcccgcgg taatcgcctc cttggcgctg
     4081 atcgctgcgc tcggcgcgat gctcgttcag ggggctgcgc tcgcgccgcc tgcggccgcc
     4141 gtaggcccgg cgccattctc gttctcgacc ttctccgtcg cgttcatgac gcagtactgg
     4201 ttccacttcg aggtcgcttc ggtcctgctc gtcatcggga tcgtcgccgc ctggacggtg
     4261 atcacggagc gtcgctgatg gacaggtatg agttcctcat cgcgctcgcc gtgctcatgt
     4321 tcgcgaccgg tctcggcggc gtggtgatcc gccgtaacct cctggtcgtc gtgatgtgcg
     4381 tcgaggtgat gctcaattcg gtggtcctcg cctttgtcgc attcgcggcg cgcacgggca
     4441 cgctggccgg ccccgcgatg accttcttca tctacattgc ggcctcctgc gaaatcgcct
     4501 tggccatggc gatcgtcgtg aggctcgtcg agcggcgcgg aacgctcgac ctcgcggccg
     4561 actacgagct gaagggctga gatgaagccg ccacgctcac ttcgatcgct gccccccggg
     4621 ggggcgccca gctcctgcgg aacggccggg cggtactgag atggcctcgc tcgcgctcat
     4681 tgccgcgctg ccgctgctgg gcttcctcgt caacgggctc ttcggcgcgc ggctgccccg
     4741 gccgctggtc gc
//
LOCUS       DATDEU010000203         3810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_35853, whole genome shotgun sequence.
ACCESSION   DATDEU010000203 DATDEU010000000
VERSION     DATDEU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35853"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VIV63_04990"
     CDS             <1..181
                     /locus_tag="VIV63_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY6123985.1"
                     /translation="VSGWYFANPEAKYFAVGPIDLDQMQHYAARKGWSASEARKWLVS
                     NLEGRVTADPHDEAA"
     gene            complement(186..608)
                     /locus_tag="VIV63_04995"
     CDS             complement(186..608)
                     /locus_tag="VIV63_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123986.1"
                     /translation="MQMIWLGTAIAGTVGYHLVLKLIPAGANPFLSLAVTFAIGSMAF
                     MGIYAVAPGSAPLRESFTQLNWTTVVLAAVIVLLDLSFLMLYRSGFEVSLGQLISQSA
                     AALLLLLLGVAFFAEKLSLANIAGILLCIAGLWLINRK"
     gene            692..1906
                     /locus_tag="VIV63_05000"
     CDS             692..1906
                     /locus_tag="VIV63_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY6123987.1"
                     /translation="MTIPDGDNAAEQRLTFGRYVLDLRRGSLLLDGREVALRPKTFAV
                     LRFLVENPGRLVSKEELLEAVWPNLVVTDDTLVQSIGELRRTLGEAGARLIVTVPRRG
                     YRFEATEAPPDRRKARGIGALRFRWKYGILAPLAIGITVLAMWLVPKLLDASDPPVAI
                     AAKPAIAVLPFQNQGDDPTREYLADGITQDIINSLGRFSALTVMSWNAVAPYKGAAVR
                     PGEIARVLAVRYQVEGSVNYTDNRLRVSAQLVDDQGRVLWSSRFDEAPTDVFALQDRI
                     TREIAGALAIRVTQFEQRRVSAKPTSSFDAYDFVLRARPALQRPERARIVEARALLRQ
                     AIALDPNYAAAHSALGETFHAAISLGWAESPDEYWKRVEYQANEALRLDETDVRARIL
                     LARRLISYHRYK"
     gene            1985..2359
                     /locus_tag="VIV63_05005"
     CDS             1985..2359
                     /locus_tag="VIV63_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123988.1"
                     /translation="MWLGKTDAAIETLELAQRIDPELNAFDRFALSLAYYLKRRYAES
                     IEQAELNQHKTPAANFNVVMLAAAYAQADRPADAARMVDNLRRADPTFDAYTFGNKFL
                     NPQDLAQLREGLRKAGLFDPDP"
     gene            complement(2356..2682)
                     /locus_tag="VIV63_05010"
                     /pseudo
     CDS             complement(2356..2682)
                     /locus_tag="VIV63_05010"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
     gene            2919..>3810
                     /locus_tag="VIV63_05015"
     CDS             2919..>3810
                     /locus_tag="VIV63_05015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY6123989.1"
                     /translation="MKRTNSFYGLLLITLLGASAPAWADAVTDWNEITVQAVTTGRPG
                     PIGIVDIALVQVAVHDAVQAIEQRFEPYHVELDARPGQRLKGRLSAAVAAAAHDVLVG
                     MYPAQAASLDTTYFNYLADKGLNDDPGIEIGQRVAERTLPLRRVNPDPLPPPFIGGTG
                     VGAWRPTPSFLGNPPSPPPFSPMAASWMAKFDPFTLTSASRFRAPPPPALTSERYARD
                     YDEVKEFGSLVGGKRTAAQTDLAYFYSDNIPAQWNRALRGIATQQLRRSGDTARLFAL
                     ANLATADALISSWDSKTFYNV"
BASE COUNT          711 a         1248 c         1215 g          636 t
ORIGIN      
        1 ggtcagcggc tggtacttcg cgaatcccga agcgaagtac ttcgctgtcg ggcccatcga
       61 cctcgaccag atgcagcact acgccgcgcg caagggctgg tcagctagcg aggcgcgcaa
      121 gtggctcgtc agcaatctcg agggacgggt taccgccgat ccgcacgacg aagcggcgta
      181 actgcctact tccgattgat gagccacaag cctgcgatac acagcaggat gccggcaatg
      241 ttcgcaagcg acagcttctc ggcaaagaac gccacgccga gaagcaggag cagcagcgcc
      301 gcggccgact gggagatcag ctgcccgaga ctgacttcga agccactgcg atagagcatc
      361 aggaagctca ggtcgagcaa cacgatgacc gcggcgagca ccaccgtggt ccagttcagc
      421 tgcgtgaaag actcgcgcag cggcgcggaa cctggcgcca ccgcgtagat gcccatgaat
      481 gccatgctgc ctattgcgaa ggtgacggcc agtgaaagaa acgggttcgc gccggcaggt
      541 atcagcttga gcaccaggtg ataaccgacg gtgcccgcga tcgcagttcc gagccagatc
      601 atttgcatgg acgccatcct cctttgtccg gcgcgtagtt tgccgcccgg acgattgcga
      661 agccattggc aggacctcat gatcgtccgc gatgaccatc cccgacggcg acaacgcggc
      721 ggagcagcgg ctgactttcg gccgctacgt gctcgacctt cgccgcgggt cgttgctgct
      781 cgatgggcgc gaagtcgcgc tgcggccgaa gaccttcgcg gtgctgcgtt ttctcgtcga
      841 gaatccgggc cggctcgtct ccaaggaaga attgctcgaa gcggtgtggc cgaatctcgt
      901 cgtcaccgac gacacgctgg tgcagagcat cggtgaattg cggcgcacgc tcggcgaggc
      961 cggcgcccga ttgatcgtca ccgtgccgcg ccgcggctac cgcttcgaag ccaccgaggc
     1021 accgcccgac cggcgcaagg cgcgcggcat cggcgcgctg cgcttccgct ggaagtacgg
     1081 catcctggcg ccgctcgcga tcggcatcac ggtgctggcg atgtggctgg tgcccaagct
     1141 gctggacgca tcggatccgc cggtcgcgat tgccgcaaaa cctgccatcg ccgtgctgcc
     1201 gttccagaac cagggcgacg atccgacacg tgaatatctt gccgatggca tcacgcagga
     1261 catcatcaac tcattgggac gtttctcggc actcacggtg atgtcgtgga atgcggtggc
     1321 gccttacaaa ggcgcggcag tgcgcccggg agaaatcgcg cgcgtgctcg cggtgcgtta
     1381 ccaggtcgaa ggcagcgtga actacacgga caatcgcctg cgcgtcagcg cccagctcgt
     1441 cgacgatcag ggccgtgtgt tgtggtcgtc gcgtttcgac gaggcgccca ccgatgtgtt
     1501 tgcgctgcag gaccgcatca cgcgcgaaat cgcgggcgcg ctcgcgatcc gcgtcacgca
     1561 gttcgagcaa cggcgcgtat cggcgaaacc gacgtcgagc ttcgatgcct acgacttcgt
     1621 gctgcgcgcg cggccggcgc tgcagcggcc ggagcgtgcc cgcatcgtcg aagcgcgtgc
     1681 gctgctgcgc caggccatcg cgctcgatcc gaactacgcc gccgcgcatt cggcgctcgg
     1741 cgaaaccttc catgcggcga tatcgctggg ctgggcggag tcgcccgatg agtactggaa
     1801 gcgcgtcgag tatcaggcca acgaggcgct gcggctcgac gaaaccgacg tgcgcgcgcg
     1861 cattctgctg gcgcgccgac tgatctccta ccaccgctac aagtaagccg aggcgcagat
     1921 cgatcgcgcg atcgcggtca atccgaacga cgccgacgcg ctcgcgggcc gcggcaacat
     1981 actcgtgtgg ctcggcaaga ccgacgctgc catcgaaact ctcgagctgg cgcagcgtat
     2041 cgacccggag cttaatgcgt tcgaccggtt cgcattgagc ctggcttact acctcaagcg
     2101 ccgctatgcc gaaagcatcg agcaggccga gctgaaccag cacaagacgc cggccgcgaa
     2161 cttcaacgtg gtgatgctgg ccgcggccta tgcgcaggct gatcgtccgg cggacgcggc
     2221 gcgaatggtg gacaacctcc ggcgcgccga tcccaccttc gacgcctaca cgttcggcaa
     2281 caagtttctc aacccgcagg atctggccca attgcgcgaa gggttgcgca aggccgggtt
     2341 gttcgatccc gacccctaac gtgtcggcag catcgggcgg tgctcgttgg cgcgctcgat
     2401 attctgattc aggcccttgc gaaacaattc ggggcccgcc ggtttgccga agagatacaa
     2461 cttcccgtca ctgatcagcc agtactcggg attggcttcg cgcacttcgc cgcgcgcgag
     2521 cgccaccgcg cagaagttcg cgaattgcgg cgcgtaacgc accgggtccg ccttgaacag
     2581 gtcgcgattc tgcgcgctcg agaacagcca gcgatgctca tcccagtcgt gcgcgaagtg
     2641 cgcgtagccg ggtgtcgcgc gctgcagcgt gaagtaggcg accgcgtcat agccctagat
     2701 ggcgagcaag tccgactcat gcgcctcgac ggagccacag agcagtacgc ctggcgtgag
     2761 cgaaagaaag actcggcgcg aaacgatgga catggaacca aacatacctc cggaatggcc
     2821 gcgtgcgtca tcattcggcg cagtttcggc gtttcttccg ggttccttcg ttacgccagc
     2881 cggcaaatcg cttctcctgc gtgccggagg aacaaaccat gaaacgaacc aatagttttt
     2941 acgggctcct gttgatcacc ctgctcggag catcagcacc cgcctgggcc gacgcggtga
     3001 cggactggaa tgagatcacc gtgcaggcgg tcacgaccgg acgcccgggt cccatcggta
     3061 tcgtcgacat cgcgttggtg caggtcgcgg tgcacgatgc cgttcaggcc atcgaacagc
     3121 gcttcgagcc ttatcacgtc gaactcgacg cacggccggg ccaaagactc aagggcagac
     3181 tgtccgcggc ggtcgccgct gcggctcatg acgtgctcgt cggcatgtat ccggcccagg
     3241 ccgcgtcgct cgacacgacg tacttcaact acctcgccga caaaggtctg aacgacgatc
     3301 ccggaatcga gatcgggcag cgggtcgccg agcggactct gccgctgcgg cgtgtcaatc
     3361 ccgatcccct gccaccgcca ttcatcggcg gcaccggggt gggtgcctgg cgaccgacgc
     3421 cgtcattcct cggcaacccc ccgtcacctc cgccgttctc gccgatggcg gcatcgtgga
     3481 tggcgaagtt cgatcccttc acgctcacga gcgcatcgcg atttcgcgct ccgccgccgc
     3541 cggcgttgac cagcgaacgc tacgcgcggg actacgacga agtgaaggag ttcggatcac
     3601 tggtcggcgg caaacgcacc gccgcgcaga cggatctcgc ctatttctac agcgacaaca
     3661 tcccggcgca gtggaaccgc gcgctgcggg gcatcgccac gcaacagctg cggagaagcg
     3721 gcgatacggc gaggctgttt gcgctcgcga acctggccac ggccgatgcg ttgatatcga
     3781 gctgggacag caagacgttt tacaacgtct
//
LOCUS       DATDEU010000204         3358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_619904, whole genome shotgun sequence.
ACCESSION   DATDEU010000204 DATDEU010000000
VERSION     DATDEU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619904"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..577)
                     /locus_tag="VIV63_05020"
     CDS             complement(20..577)
                     /locus_tag="VIV63_05020"
                     /inference="COORDINATES: protein motif:HMM:NF023089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HEY6123990.1"
                     /translation="MRKLAEALIASAWLTLCACASVPPQAETAQRPLVAPASLGSDRV
                     VSQVVRGAFGAREITIHCVVTVKDGRMTLVGLNSLGLRLFTVSYDGKAVTSETASAMS
                     GPLMPERLLADLQLVFWPLTSLEKPMASAGWSLSEPAAGIRRLRRGDQLVAEAHYGSQ
                     DPWSGRSWLVNFEFGYSLQIDSQAL"
     gene            complement(574..1797)
                     /locus_tag="VIV63_05025"
     CDS             complement(574..1797)
                     /locus_tag="VIV63_05025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6123991.1"
                     /translation="MVDVLIIGAGPSGSMAAALLASRGYEVLVLEKQQFPRFSIGESL
                     LASTAELLSEAGMLDAVMACGFQYKNGAAFVRDDLYSEFNFAEKFSAGYPFTFQVPRA
                     QFDQVLANEAARKGATIRHEVEITAIDVSGERPVVTAKAGDEITQHRPRFVLDASGFG
                     RTLPRLLDLDRPSDFPERAALFVHVKDNIVSGEFDRQKIRVGVHPTQGDVWSWLIPFS
                     NGNASLGVVASREHHAKFTGTNEERLWKLIAEEPPLKKLLKDAHTVRPVGEITGYAAK
                     VSRLHGPGFALLGNAAEFLDPVFSSGVTIALKSAKLAADLLDRQFRGESTDWETEFAA
                     PLMYGVETFRSFVNGWYNGDLQEVIFFEQKDPVIRRMICSVLAGYAWDAKNPYTGPQS
                     ARRLRALAETVRAVR"
     gene            complement(1790..>3358)
                     /locus_tag="VIV63_05030"
     CDS             complement(1790..>3358)
                     /locus_tag="VIV63_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6123992.1"
                     /translation="SIETIAVVLLLLLVFASMRPLLLSVLTLALAITAAFTVVHLVFG
                     KVHILALVFGSSLIGSVIDYSIHFFADRFREPSQWTPAAALPHVGPAILLGLTTTLVG
                     YLVLAAVPFPGLEQIAVFCITGLVVGCGSVLCLYPVLAHSGGTVPVLGPRIGTAIDRV
                     LQGWRWTNTKWALTATLLIVVALGLARVKTQDDIRALQQSPAQLVNEEQRVRELLGSG
                     IETRFFLVTGESAQAVLETEERLAQALEGLEREGVIAAWQGVSTSVPSIAIQRRNHEL
                     LAQRVYAPGQLLERVMGTLGFEPAAIERRRAEFEAAEAPLTIDEWLGSPSSQGARHLW
                     LGQVGSRVATVVTLGGITDVSALTKVQIPGVQLIDRVAQTTQTLSRYRQVMTLLLAAV
                     YAAAGVILVVRFGWRDMPRMLLPSVAATLTTIGIFGWLGVPFNLFTLLALWLMLGLGI
                     DYGIFLRHGRHHRVTAILSVTLSACTTLIAFGLLAFSATPFIRSIGLTLLFAIPLSWL
                     FALLACMTEKKIHG"
BASE COUNT          648 a         1117 c         1056 g          537 t
ORIGIN      
        1 gtctgaagca gccatcgaac tacaaggcct gactatctat ctgaagcgag tagccgaact
       61 cgaaattcac gagccagctc cggccgctcc acggatcctg gctgccgtag tgcgcctccg
      121 ccacgagttg atcgccgcgg cgcaggcggc gaatgcccgc cgccggttcg ctcaacgacc
      181 agccggccga ggccatcggc ttttcgagac tggtcagcgg ccagaagacg agctgcagat
      241 ccgccagcaa tcgctccggc atcaagggtc ccgacatcgc ggaggcggtc tcggatgtca
      301 cggctttccc gtcgtagctg acggtgaaca ggcgcaagcc gagcgagttg agacccacga
      361 gcgtcatgcg accgtccttc accgtcacca cgcagtggat ggtgatctcg cgcgcgccga
      421 atgctccacg cacgacctgg ctcacgacgc gatcgctgcc gagcgatgcg ggcgcgacca
      481 ggggacgttg tgcagtctcg gcctgcggcg gaacactggc gcatgcgcaa agcgtcagcc
      541 acgcagaggc aatcaacgct tccgccagct ttctcatcgc accgcccgga cggtttccgc
      601 cagcgcgcgc agccggcgcg cggactgcgg gcctgtatag ggattcttcg cgtcccaggc
      661 ataacccgcg agcacggaac agatcatgcg tcggatgact ggatcctttt gctcaaagaa
      721 gatgacctcc tgcaggtcgc cgttgtacca gccattgacg aaactgcgaa aggtttcgac
      781 gccgtacatg agcggcgcgg cgaactccgt ttcccagtcg gtcgactcgc cgcggaattg
      841 cctgtccagc aggtcggcgg ccagtttcgc ggacttcagc gcgatggtga cgcccgagga
      901 aaagaccgga tccaggaatt cggccgcgtt gcctaacaac gcgaaacccg ggccgtgcag
      961 ccggctcact ttcgccgcgt agccggtgat ctcgcccacc ggtcgcacgg tgtgcgcatc
     1021 tttcagcagc tttttcagcg gcggttcctc ggcgatgagc ttccacaacc gctcttcgtt
     1081 cgtccccgtg aacttcgcgt ggtgttcgcg ggaggcgacc acgccgagcg aggcgttccc
     1141 gttcgagaac ggtatcagcc acgaccagac gtcaccctgc gtcggatgca cgccgacacg
     1201 aatcttctgg cggtcgaact cacccgacac gatgttgtcc ttcacgtgga cgaacagcgc
     1261 ggcgcgctcg ggaaaatccg acggccgatc gagatcgagc agtctcggca aggtgcggcc
     1321 gaagccgctg gcgtcgagca cgaatctggg ccggtgctgc gtgatttcgt cgcccgcctt
     1381 cgcggtgacc accggacgct cgcccgaaac gtcgatggcg gtgatctcca cctcatgacg
     1441 gatcgtcgcg cctttacgcg cagcctcgtt cgccagcacc tggtcgaact gcgcacgcgg
     1501 cacctggaac gtgaacggat aaccggccga gaatttttcg gcgaagttga attcggaata
     1561 cagatcgtca cgcacgaagg cggcgccgtt cttgtactgg aagccgcagg ccatgaccgc
     1621 gtcgagcatg cccgcttcgc tcaacagttc cgccgtgctc gcgagcaggc tctcgccgat
     1681 cgagaagcgc gggaattgct gcttttcgag cacgagcacc tcgtatccgc ggctcgccag
     1741 caatgccgcc gccatcgagc ccgacgggcc cgcaccgatg atcaacacat caaccatgga
     1801 tctttttctc cgtcatgcag gccagcagtg caaacaacca ggacaggggg atcgcgaaca
     1861 gcagtgtcag gccgatgctg cgaatgaaag gtgtggcgct gaacgccagc agcccgaatg
     1921 cgatgagcgt cgtgcacgcc gacagcgtga cgctgagaat ggccgtgacg cgatgatggc
     1981 ggccgtggcg caggaagatt ccgtagtcga tgccgagtcc gagcatcagc cacaacgcga
     2041 gcagcgtgaa gagattgaag gggacgccga gccagccgaa gattccgatc gtggtgagcg
     2101 tggccgccac cgacggcagc aacatgcgcg gcatgtcgcg ccagccgaaa cggaccacga
     2161 ggatgacgcc ggcagcggca tacacggccg ccagcaacag cgtcatcacc tgtcgatagc
     2221 ggctcagcgt ctgcgtggtc tgcgcaacgc ggtcgatgag ctgcacgccg ggtatctgca
     2281 ccttcgtcaa tgccgataca tcggtgatgc cgcccagagt cacgacggtc gcgacgcgcg
     2341 atccgacctg gcccagccac aggtgcctgg caccctgcga cgacgggctg ccaagccatt
     2401 cgtcgatcgt gagcggcgcc tccgctgcct cgaattccgc gcggcgccgt tcgatggccg
     2461 ccggctcgaa ccccagcgtg cccatcaccc gctctaacaa ctggcccggc gcgtatacac
     2521 gctgggccag cagctcgtgg ttgcggcgct gaatcgcgat cgacggcacg ctggtcgata
     2581 cgccctgcca ggccgcgatg acgccctcgc gttccagacc ctcgagcgcc tgcgcgagac
     2641 gctcttcggt ttcgagcacg gcctgcgccg actcgcccgt cacgaggaag aagcgggttt
     2701 cgatgccgct gcccaggagt tcgcgcacgc gctgttcttc gttcaccagt tgcgccggcg
     2761 actgctgcaa tgccctgatg tcgtcctggg ttttcacgcg cgccaggccc agcgcaacca
     2821 cgatgagcag cgtggccgtt aacgcccact tcgtgttcgt ccagcgccag ccttgcagca
     2881 cgcgatcgat cgccgtgccg atccgcggcc cgagtacggg taccgtgccg cccgagtggg
     2941 cgagcacggg atacagacac aacacgctgc cgcagcccac cacgagtccc gtgatgcaga
     3001 acacggcgat ctgttcgagc ccgggaaacg gcactgcggc cagcaccagg taaccgacca
     3061 gcgtagtagt gaggcccagc aggatggccg gacccacatg gggcagagcc gctgccggcg
     3121 tccactgcga gggttcgcga aagcggtcgg cgaagaagtg gatcgagtag tcgatgacac
     3181 tgccgatgag gctcgagccg aagaccagcg cgaggatatg gaccttgccg aacacgaggt
     3241 gcaccaccgt aaacgccgcg gtgatcgcca gcgccagcgt cagcacgctg agtaatagcg
     3301 gccgcatcga ggcgaagacg agtaacagca acaacaccac cgcgatcgtc tcgatgga
//
LOCUS       DATDEU010000205         5523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2078851, whole genome shotgun sequence.
ACCESSION   DATDEU010000205 DATDEU010000000
VERSION     DATDEU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078851"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VIV63_05035"
     CDS             complement(<1..1321)
                     /locus_tag="VIV63_05035"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY6123993.1"
                     /translation="MRAVLEPADLERYNELNDTQGAMRAHWRPLIDRLRADDSPDAVR
                     RSLELTRRLVVENGVTYNVYADPQGADRPWALDPLPFVLPAAEWKTIEAGVAQRARML
                     NALLADLYGPQRLLAEGHLPAELPFGHPNYLWPCHDLRPADGAWLHVYAADLARAPDG
                     RWWLLADRTQAPSGAGYALENREILEQVHPEAIPDMSVRRVRGFFGALRERMLDAAGD
                     ESPLAVILTPGPYNETYFEHAYLARQLGMPLVEGHDLTVRGDTVYLKTLGGLRRVHSI
                     LRRLDDDYCDPLELRGDSALGVPGLIGALRAGRVVVSNALGTGVLESAAWLGFLPPIA
                     ERLIGETLLLPEIATWWLGEKPALEYVLANLDRLVIKPTYPNQRFEPMFGREFEGRAR
                     DLLIERLRNRPYAYVAQEHVRLSQAPVWKSAATSELGERALTIRVYAV"
     gene            complement(1340..4675)
                     /locus_tag="VIV63_05040"
     CDS             complement(1340..4675)
                     /locus_tag="VIV63_05040"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY6123994.1"
                     /translation="MSIHVALNHVTHYRYDRLVALGPQVVRLRPAPHCRTRIISYSLK
                     VTPSKHFINWQQDPEANYLARYVFAEKTKELRIEVDLVAEMAVLNPFDFFLEPYAETF
                     PFAYQGVDKRDLAPYLVMFRGEPKFKAYLGTIPRDRKRTIDFLVELNAKLANDIKYLI
                     RMEPGVQTPELTLTKCSGSCRDSAWLLVQLLRHMGLAARFVSGYLIQLTADMKSLDGP
                     SGPEKDFTDLHAWCEVYLPGAGWVGLDPTSGLLAGEGHIPLACTPEPGAAAPVTGAVD
                     KAEVEFEHHMKVERIWEAPRVTKPYTEEQWKEIELLGHAIDAQLIAGDVRLTMGGEPT
                     FVSVDDPDGAEWNTTAMGPDKRRLAIDVYSRLKEKYAPQGLSHFGQGKWYPGEQLPRW
                     SLNCFWRRDGEPIWQNPALLDNEKEDRGITAEIAGVFLAGVAERLGLPPEYVFAAFED
                     AFYYMWRERRLPSNVDPFKSKLEDSVERARLARIFEQGLDAVVGHVLPVSRHVTGDRW
                     QTGDWFLRRERCYLVPGDSPLGLRLPLDSMPWVKKEDYPYTSPPDPMAPPSPLAAQAE
                     IRRQLRETEVRDAKAAATRTSGKARDGVVSPSMVPLAQKSATEITRTAMCAEPRNGVL
                     YVFMPPTDKLEDYLELVAAVEATAEALDTPVVLEGYEPPKDPRLNRFSVTPDPGVIEV
                     NVHPSGSWDELVDRTTHLYDAARQCRLTTEKFMIDGRHTGTGGGNHFVLGGATPADSP
                     FLRRPDLLRSLLAYWHNHPALSYLFSGLFVGPTSQAPRIDEARNDSLYELELAFKQLP
                     PLGQPVAPWVVDRLFRNLLIDSTGNTHRSEFCIDKMFSPDGNTGRLGLLEMRAFEMPP
                     HERMSLTQQLMLRSLIARFWREPYEPARLARWGTELHDRFMLPYFIDRDLNDVIAEQN
                     AAGFPLKAEWFAPHAEFRFPKYGDFSVYGAEVELRQALEPWHVMGEEGAAGGAVRYVD
                     SSVERLQVLVTGLAPDRYALTCNGRRIPLRSTGPVGEFVAGVRYKAWAPPSALHPLIP
                     AHSPLIFDLVDTWMNRSMGGAQYHVAHPGGRSHETFPVNAFESESRRLARFVRMGHTP
                     QKVTPQVDTPNIEYPFTLDLRKV"
     gene            4835..>5523
                     /locus_tag="VIV63_05045"
     CDS             4835..>5523
                     /locus_tag="VIV63_05045"
                     /inference="COORDINATES: protein motif:HMM:NF016090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY6123995.1"
                     /translation="MKTDWTSYPSPGFHDELVGPSGGARAAGRQLMRYLTDMDGNEIA
                     VRQQAAELAIKAMGITFTVYHEQDGSIDRAWPLDIIPRTITRKEWQKIEAGLIQRAAA
                     LNLFINDVYHEQKFIKDGLMPASIIATSKNFRASARGIKPPLGTWAHICGSDLIRDKD
                     GTVYVLEDNLRVPSGVSYMLENRQVVKRVFPELFESAAILPVDDYPSQLYDMLAALSP
                     RKETRPEVVVLT"
BASE COUNT          993 a         1735 c         1862 g          933 t
ORIGIN      
        1 ccacggcgta cacgcggatc gtgagcgcgc gttcaccgag ctcggaagtc gccgcggatt
       61 tccagaccgg cgcctgggag aggcgtacgt gttcctgcgc gacgtaggcg tatgggcggt
      121 tgcgcagccg ctcgatcagc agatcgcgcg cccggccttc gaactcgcgg ccgaacatcg
      181 gctcgaagcg ctgattcggg taggtgggct tgatgaccag ccggtcgaga ttggcgagca
      241 cgtattcgag cgccggcttc tcgcctaacc accaggtggc gatctccggc agcagcagcg
      301 tttccccgat gagcctttcg gcgatcggcg gcaggaagcc gagccaggca gcggactcga
      361 gcacaccggt gccgagcgcg ttcgacacca cgacgcgccc ggcgcgcaac gcgccgatga
      421 gaccgggcac accgagcgcc gagtcgccgc gcagttcgag cggatcgcag tagtcgtcgt
      481 cgagacggcg caggatcgag tgcacgcggc gaaggccgcc gagcgtcttg aggtacaccg
      541 tgtcgccgcg cacggtgaga tcgtggcctt cgaccagcgg catgccgagc tggcgggcca
      601 ggtaggcatg ctcgaaatag gtttcgttgt acggtccggg cgtgaggatg acggcgagcg
      661 gcgattcatc tcccgcggcg tcgagcatcc gttcgcgcag ggcgccgaag aagccgcgca
      721 cgcggcgcac cgacatgtcg ggaatggcct cgggatggac ctgttcgagg atctcgcgat
      781 tttcgagcgc ataaccggcg ccactcggcg cctgcgtgcg atcggcgagc agccaccagc
      841 ggccatcggg cgcgcgcgcc aggtcggcgg catagacgtg cagccaggca ccatcggccg
      901 gacgcaggtc atggcagggc caaaggtagt taggatggcc gaaaggcagt tcggccggca
      961 ggtgcccttc ggcgagcagt ctctgcggac cgtagagatc cgccaggaga gcattcagca
     1021 tgcgcgcgcg ttgcgccacg ccggcttcga tggtcttcca ttcggcggct ggcagcacga
     1081 agggcaacgg atcgagcgcc cacggacggt cggcgccctg cgggtcggca tacacgttgt
     1141 aagtgacgcc gttctcgacg acgaggcggc gcgtgagttc gagcgaacgg cgcacggcgt
     1201 cgggcgaatc gtcggcgcgc agacggtcga tgagcggccg ccagtgtgcg cgcatcgcgc
     1261 cctgggtatc gttcagctcg ttgtatcgtt caaggtcggc gggctcgagt acggcacgca
     1321 tttcactaat gctcacccat caaactttac gcagatcgag cgtgaacggg tactcgatgt
     1381 ttggcgtatc cacctgcggt gtgaccttct gcggcgtgtg acccattctg acaaatcgcg
     1441 ccaggcgacg gctttcggat tcgaacgcgt tcaccgggaa ggtttcgtgg cttcggccgc
     1501 cgggatgcgc gacgtgatat tgcgcgccgc ccatcgatcg attcatccag gtatcgacca
     1561 gatcgaagat cagcggtgaa tgtgccggga ttagcggatg cagcgcggaa ggcggcgccc
     1621 aggccttgta acgcacgccc gcgacgaact cgcccaccgg tccggtggag cgcaacggga
     1681 ttcgtcgccc gttgcaggtg agcgcgtatc gatcgggtgc gaggccggtg accagcacct
     1741 gcagtcgctc caccgacgaa tccacgtaac gcaccgcgcc gccggcagcg ccctcttcgc
     1801 ccatgacgtg ccagggctcg agcgcctggc gcagctcgac ttccgcgccg tagaccgaga
     1861 agtcgccgta cttggggaaa cggaattccg catgcggcgc gaaccattcg gccttgagcg
     1921 gaaagcccgc ggcattctgc tcggcgatga cgtcgttgag atcgcgatcg atgaaatacg
     1981 gcagcatgaa gcggtcatgc agttcggtgc cccagcgcgc gaggcgcgca ggctcgtagg
     2041 gctctcgcca gaaacgagcg atcaaggatc gcagcatgag ctgctgcgtg agactcatgc
     2101 gctcgtgcgg cggcatctcg aaggcacgca tctcgagcaa ccccaggcgg ccggtgttgc
     2161 catcgggcga gaacatcttg tcgatgcaga actcggagcg atgcgtgttg cccgtggagt
     2221 cgatgagcag gttgcggaac agccggtcga cgacccacgg cgccacgggc tggcccagcg
     2281 gcggcagttg tttgaacgcc agctcgagtt cgtacaacga atcgttgcgc gcctcgtcga
     2341 tgcggggcgc ctgcgaggtg ggacccacga acaaaccgct gaacagatag gacagcgcgg
     2401 gatggttgtg ccagtaggcc agcagactgc gcagcagatc gggccggcgc aggaacggcg
     2461 aatcggccgg cgtggcgccg cccagcacga agtggttgcc gccgccggtg ccggtatggc
     2521 ggccgtcgat catgaatttt tcagtagtca ggcggcactg gcgcgcggca tcgtagagat
     2581 gcgtcgtgcg atccacgagt tcatcccagc ttccggacgg gtggacgttc acctcgatga
     2641 cgcccggatc cggcgtcacg ctgaaccggt tgagtcgcgg atccttcggt ggctcataac
     2701 cttcgagcac caccggagtg tcgagcgcct cggcagtcgc ttcgacggcc gcgacgagct
     2761 ccaggtaatc ctcgagcttg tccgtgggcg gcatgaacac gtagagcacg ccgttacgag
     2821 gttcggcgca catcgcggtg cgcgtgatct ccgtcgccga cttttgcgcc agcggcacca
     2881 tgctgggaga aacgacgcca tcgcgcgcct tgccactggt acgggtcgcc gccgccttcg
     2941 cgtcgcgcac ttccgtctct cgaagctggc ggcggatttc cgcctgcgcg gcgagcggac
     3001 tcggcggcgc catgggatcg ggcgggctgg tgtatggata gtcttctttc ttgacccacg
     3061 gcatggagtc gagcggcagc ctcaatccca gcggcgaatc gccgggaacg agatagcagc
     3121 gctcgcgccg caggaaccag tcgccggtct gccagcggtc gccggtgacg tggcgtgaca
     3181 caggaagaac gtggccgacc accgcgtcga gcccctgctc gaatattcgc gccaggcgcg
     3241 cgcgttcgac cgagtcttcg agtttcgact tgaacggatc gacgttggac ggtagacggc
     3301 gttcgcgcca catgtaatag aaagcgtctt cgaacgccgc gaacacgtat tcaggcggca
     3361 ggcccagtct ttcggcgaca cccgcgagga acacgccagc gatctcggcg gtgatgccgc
     3421 ggtcttcctt ctcgttgtcg agcagcgccg ggttctgcca gatgggctca ccgtcgcggc
     3481 gccagaagca gttgagcgac cagcgcggca actgttcgcc gggataccac ttgccctggc
     3541 cgaagtgcga cagaccttgc ggcgcgtatt tctccttgag ccggctgtac acgtcgatgg
     3601 cgaggcggcg cttgtcgggc cccatcgccg tggtgttcca ctccgcgcca tccggatcat
     3661 cgacagacac gaacgtgggc tcgccgccca tcgtgagtcg cacgtcgccg gcgatcagtt
     3721 gcgcgtcgat ggcgtggccg agtaattcga tctccttcca ctgctcttcg gtgtaaggct
     3781 tggtgacgcg cggcgcttcc cagatgcgct ccaccttcat gtggtgctcg aactcgacct
     3841 cggccttgtc gaccgccccg gtgaccggcg ccgcggcgcc aggctcgggc gtgcacgcga
     3901 gcggaatgtg gccttcgccc gcgagcagtc cggaggtggg atcgaggccg acccagccgg
     3961 cgccaggtag atagacttcg caccaggcat gcaggtcggt gaaatccttc tccggacccg
     4021 acgggccgtc cagcgacttc atgtcggcgg tgagctggat caggtagccg gagacgaagc
     4081 gcgccgcgag acccatgtgg cgcaagagct ggaccagcag ccaggccgaa tcgcggcacg
     4141 aacccgaaca cttggtgagc gtgagctcgg gcgtctgcac gccgggctcc atgcggatga
     4201 ggtacttgat gtcgttcgcg agcttggcgt tgagctcgac cagaaagtcg atggtgcgct
     4261 tgcggtcgcg cggaatggtg ccgaggtagg ccttgaactt cggctcgccg cggaacatca
     4321 cgaggtacgg cgcgaggtcg cgcttgtcca cgccctggta ggcgaacgga aaagtctcgg
     4381 cgtacggctc gaggaagaag tcgaaaggat tgagcaccgc catctcggcg accaggtcca
     4441 cttcgatgcg cagctctttc gtcttctcgg cgaacacgta acgcgcgagg tagttagcct
     4501 ccgggtcctg ctgccagttg atgaagtgct tggaaggcgt gaccttcagc gagtaggaga
     4561 tgatgcgcgt ccggcagtgc ggcgcgggcc gcaggcggac gacttgtggc cccagcgcca
     4621 cgaggcggtc gtagcgatag tgcgtgacgt gattgagcgc gacatgtatg gacacgcggc
     4681 cattataggc agaggtctaa cacacgcgtt cgcaccaatt tagccagaaa acagcatcac
     4741 ggcaccatcg ggatgcgcgc agactgaggc ctcgcacttt tttgaacacg gccttttggc
     4801 tctggtattt ttgggccgag aacgcacgcc aacaatgaaa accgactgga cgtcataccc
     4861 ttcccctgga ttccacgatg agctcgtggg cccgagcggc ggcgcacgcg cagccgggcg
     4921 ccagctgatg cgttatctga cggatatgga cggcaacgag atcgccgtgc gccagcaggc
     4981 ggccgaactg gccatcaagg ccatgggcat aaccttcacg gtctaccacg agcaggacgg
     5041 gtcgatcgac cgcgcgtggc cgctcgacat catcccccgg accattaccc gcaaggaatg
     5101 gcaaaagatc gaggcaggcc tcatccagcg cgccgccgcg ctcaatctct tcatcaacga
     5161 cgtctaccac gagcagaaat tcatcaagga cggcctcatg ccggcctcga ttatcgcgac
     5221 atccaagaat ttccgggcaa gcgccagggg catcaaacca ccgctcggca cctgggcgca
     5281 catctgcggc tcggacctga tccgcgacaa ggacggcacg gtttacgtgc tcgaagacaa
     5341 cctgcgcgtg ccctccggcg tgtcgtacat gctcgagaac cgccaggtgg tcaaacgcgt
     5401 gttcccggag ctgttcgaga gcgccgccat cctgcccgtg gacgactatc cctcgcagct
     5461 ttacgacatg ctggcggcgt tgtcgccgcg caaggagacg cggcccgaag tcgtggtgct
     5521 tac
//
LOCUS       DATDEU010000206         2073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_327438, whole genome shotgun sequence.
ACCESSION   DATDEU010000206 DATDEU010000000
VERSION     DATDEU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327438"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VIV63_05050"
     CDS             complement(<1..416)
                     /locus_tag="VIV63_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6123996.1"
                     /translation="MARQYFAPLFVLFAGLASAQDIPNPLIDYGAFQSGVERVGSVRE
                     NLRVTEREFLRLAADPATVILDARSAEKFRLLHVKGAKNLSLPDITEEELAKVIPSKS
                     TRILIYCNNNFVNEPQAFPSKNLAASLNVYTLNVLFA"
     gene            complement(497..>2073)
                     /locus_tag="VIV63_05055"
     CDS             complement(497..>2073)
                     /locus_tag="VIV63_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6123997.1"
                     /translation="GTDLTNPRDSDNDGTPDYLDTDSDNDTLPDCLEGSVNDNDTLLD
                     TDGDGAADLVDVDSDNDGILDSFEGTPAALTDTDGDGIPDVRDLDSDNDGLTDVTEAG
                     LIDTDADSLMDAGQTRTNLPRDTDGDGVPDFRDVATASFLAGADRDQDGIRDAVDGAP
                     QEFGTFVDRDGDGVAGSRDLDLDNDGIPDAADGSDDTDGDGRPNLADLDSDGDGITDI
                     IEAGGVDANGDGLVDNFVDVNGNGLSDSFESGLGGRALALPDTDSDGIDNHRDLDSDG
                     DSLPDSREGRGDFDNDGIPDSIDKQGSIQTAVRGAGAFDPLSLLGLLAVIAWVALRRM
                     GRGAAKALPVVAMAALASAQAGAADVLSKGWYLGFDTGMSRLEPQNDDGGYKVDDGQS
                     FGFRLTAGYAWNANWGVEGFYADGGDAGISSDNPAVGHLGDIGYKVFGVGFQWLPFDG
                     GINEQWFPLVKFGASHIQNSATSDLIVYEKLNDVGVYFGGGAGLRFGESWVAQAEVVS
                     YDADEMFFTVGLRKHF"
BASE COUNT          360 a          676 c          656 g          381 t
ORIGIN      
        1 gcgaacaaca cgttgagcgt gtacacgttg agcgacgcgg cgaggttctt ggacggaaac
       61 gcctgcggct cgttgacgaa gttgttgttg cagtagatga ggatgcgcgt ggatttcgag
      121 ggaatgacct tcgcgagctc ctcttccgtg atgtcgggga ggctcaggtt cttcgcgccc
      181 ttcacatgca gcagtctgaa cttctcggcg ctgcgcgcat cgaggatgac cgtcgccgga
      241 tcggcagcca ggcgcaggaa ctcccgttcc gtcacgcgca ggttttcgcg aaccgatccg
      301 acgcgctcca cgccactctg aaaagcgccg taatcgatga gcgggttcgg aatgtcttgc
      361 gccgacgcga gacccgcaaa caggacaaac aagggagcga agtactgcct ggccatgacc
      421 gcctccgtgg gtgacgcggt tttggacagg gaatccgcgc gcaagtgccg cgcggagtta
      481 ccttatttcg cctctctcag aagtgcttgc gaaggcccac ggtgaagaac atctcgtccg
      541 cgtcgtagct gacgacttca gcctgggcca cccagctctc gccgaagcgc agtccggcgc
      601 cgccgccgaa gtacacaccg acgtcgttga gcttctcgta cacgatcagg tccgaggtgg
      661 cgctgttctg gatgtgcgaa gcgccaaact tcacgagcgg aaaccattgc tcattgatgc
      721 cgccatcgaa cggcagccac tggaaaccga cgccgaacac cttgtagccg atgtcgccca
      781 gatgaccgac agccgggttg tcagacgaaa tgcccgcgtc gccgccatcg gcgtagaagc
      841 cctcgacgcc ccagttcgca ttccacgcgt agccggcggt gaggcgaaac ccaaagctct
      901 gaccatcgtc gaccttgtag ccgccatcgt cgttttgcgg ctcgagtcgc gacatgcccg
      961 tatcgaagcc gaggtaccag cccttgctga gcacatcggc ggcgccggcc tgcgccgacg
     1021 cgagtgcggc catcgccacc accggcagag ccttcgcggc gccgcggccc atgcggcgca
     1081 gggcgaccca cgcgatcacc gccagcagac ctaacaagct cagcggatcg aacgcacccg
     1141 cgccccgtac cgcggtctgg atgctgccct gcttgtcgat cgaatcggga atgccatcgt
     1201 tgtcgaagtc accgcggcct tcgcggctgt ccggcagcga gtcgccatcg ctgtcgaggt
     1261 cgcggtggtt gtcgatgcca tcgctgtcgg tgtccggcag ggccagcgcc cggccgccga
     1321 gaccgctctc gaacgaatcc gacagaccat tgccgttgac gtcgacgaag ttgtccacga
     1381 ggccgtcacc gttcgcatcg acaccgccgg cttcgatgat gtcggtgatg ccgtcaccat
     1441 cgctgtcgag atccgccagg ttcggccggc cgtcgccatc ggtgtcgtcc gagccatccg
     1501 cagcgtcggg aatgccgtcg ttgtcgaggt cgagatcgcg cgagccggcg acgccgtcgc
     1561 cgtcacgatc cacgaaagtg ccgaactcct gcggcgcgcc gtccacggcg tcgcgtatac
     1621 cgtcctggtc gcggtccgct cccgccagga agctggcggt agcgacatcg cggaagtcag
     1681 gcacgccgtc gccatccgta tcgcgcggaa gattggtacg tgtctggccc gcatccatga
     1741 gcgaatccgc atcggtgtcg atgaggccgg cttcggtcac gtcggtgagg ccgtcgttgt
     1801 ccgaatccag atcgcgtacg tcggggattc catcgccatc ggtatcggtg agcgcggccg
     1861 gcgttccctc gaatgaatcg aggatgccgt cattgtccga gtcgacatcg acgagatccg
     1921 cagcgccgtc gccatcggtg tccagcaacg tatcgttgtc gttcacactg ccttcgaggc
     1981 agtcgggcaa cgtgtcgttg tcgctgtcgg tgtccaggta gtcaggcgtg ccgtcattgt
     2041 cactgtcgcg cgggttggtg aggtcggtcc cca
//
LOCUS       DATDEU010000207        10499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1933206, whole genome shotgun sequence.
ACCESSION   DATDEU010000207 DATDEU010000000
VERSION     DATDEU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10499
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933206"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VIV63_05060"
     CDS             <1..374
                     /locus_tag="VIV63_05060"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY6123998.1"
                     /translation="IVIAIVGILASIAYPAYSRYIVRTHRTAAKACMAEVAQFMERYY
                     TTNMSYVDANPVAACESEAGLDQLYDIRVDDIAARAYTVTAEPIGTQATRDAQCGTLT
                     LDEAATRGKSGSADFEYCWSR"
     gene            complement(371..1132)
                     /locus_tag="VIV63_05065"
     CDS             complement(371..1132)
                     /locus_tag="VIV63_05065"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6123999.1"
                     /translation="MNFHAVQAIYRFEMSRTWRTLFQSIATPVMTTSLYFVVFGSAIG
                     SRMMSIDGVSYGAFIVPGLIMLTVLMESVSNGSFGIYMPKFAGTIYELLSAPVGLAET
                     LLGYTGAAATKSVILGLVILATARLFVPFTIEHPFWMIAFLVLTSVTFALLGFLIGIW
                     ADSWEKIQIVPMMVVTPLTFLGGSFYSISMLPPIWQKITLFNPVVYLVNGFRWSFYGK
                     SDVNVALSFGMTLGFLLLCLGVVHWMFRTGYKLKN"
     gene            complement(1129..2052)
                     /locus_tag="VIV63_05070"
     CDS             complement(1129..2052)
                     /locus_tag="VIV63_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6124000.1"
                     /translation="MSVLTVSGLSKTYASGFHALKNVDLEIRRGEIFALLGPNGAGKT
                     TLINIICGIVTASTGTVTADGHDTVRDFRAARATIGLVPQELTTDMFETVWNTLTFSR
                     GLFGKPRNPAIIEKVLRDLSLWDKKDAKIMTLSGGMKRRVMIAKALSHEPNILFLDEP
                     SAGVDVELRRDMWEMVRKLRASGVTIILTTHYIEEAEEMADRIGVISKGSLIVVEEKT
                     ALMRKLGKKQLTLQLQAPLAAIPPGLAGLPLELAKEGTELVYSFDTQASETGIAGLLR
                     KLNETGIEFKDLHTAESSLEDIFVNLVRSRP"
     gene            2161..3276
                     /locus_tag="VIV63_05075"
     CDS             2161..3276
                     /locus_tag="VIV63_05075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY6124001.1"
                     /translation="MQRSWMAYISAGLGGAALLGVISFHFPTLLTSRDFRAVYTEDFA
                     RNLLLLGLVLSFTLGTVAVLRRRSVRVALTGVCLAALAVFLGGATIELDPIRSTAFSL
                     GLDWFVISLFFSALVFIPLERMLAVRTLSPLRPEWRTDLVYFFISHVLVQFILIAVTA
                     STTTVAALVLFPAVKTWIQALPVWLQFLMAVFLADLFQAGLHRIYHKVPWLWRFHAVH
                     HSSRNLDWLAGSRVHIVEIVLTRSAVLLPLVVLGFSQPAVNAYVILVGIQAVLAHANM
                     RTHFGWLEYLLVTPRYHHWHHARHVDYMDVNYAIHLPLVDMLMGTFKRPPPDVWPDEY
                     GVMKLETVPRGIWAQTLMPMGPKKTFDDHVGAETPST"
     gene            3276..3902
                     /locus_tag="VIV63_05080"
     CDS             3276..3902
                     /locus_tag="VIV63_05080"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEY6124002.1"
                     /translation="MAVRAFLVALACLCLPTAAGAAGGADIVVAARGQVGVTVVYDPA
                     YRSLGYPGGDVPADRGVCTDVVVRALRTARAMDLQRRVHEDMKAHWDAYPHHRRWGLS
                     KPDPNIDHRRVPNLMTYFKRAGYETRLTRTAADYLPGDIVAWDLGRGILHIGIVSDVK
                     SVTGTPLVVHNIGSGAREEDILFRFTIIGHYRLPPPPVAAARASVASR"
     gene            3945..5807
                     /locus_tag="VIV63_05085"
     CDS             3945..5807
                     /locus_tag="VIV63_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6124003.1"
                     /translation="MVATPRIAKQAEVHGESLNSPRQFFGVFKYSKRALELLWTTSRP
                     LAFFLGFMTVLAGVLPSGVAWVGARIVDSVLEATRTHSAGSDVNITPVLMWVLAEALI
                     LVVITGAHRGITFCSALLKAKLSNRVNVLILEKALTLELTHFEDSEFYDKLTRARREA
                     SSRPLSLVMRTSGLFQNAIAIASFSVLLAGFSPWAVVVLIFGGLPAFFAETKFSGDAF
                     RLFRWRAPETRMQSYLETALAREDHAKEVKLFDLGKLFLGRYKAIYEGLYEKDRNLTV
                     RRDAWGFAFTLVSIAALYSAYAWCAVEAVRGVITLGEMTMYIMLFRQGQAAVTASLGA
                     IGGMYEDNLYLSTLYEYLDQPVEESKGTAVSGPDPTAGVQFEDVEFRYPGSDQVAITG
                     VNLQIRLGESLALVGENGSGKTTLIKLLTRLYKPTHGRVLLDGRDLQEWDTTVLRRRV
                     GVIFQDFVRYQLMVGENIGAGDVRAFEDEARWKEAANSGKAAPFIDDMPLRFHTQLGK
                     WFKDGRELSGGQWQKIALSRAFMRSDADILVLDEPTAAMDAAAEAEVFEHFRSLMGNR
                     IAIVISHRFSTVRMADQIVVLDQGHIVERGNHELLMKQDGIYSKLFTLQARGYR"
     gene            5887..8658
                     /locus_tag="VIV63_05090"
     CDS             5887..8658
                     /locus_tag="VIV63_05090"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY6124004.1"
                     /translation="MPTNHRVLASLTLVVALATPAWAAKKPKAAPAPPPVAAPAAPER
                     VTTVEGITEYRLANGLRVLMFPDQSKQTITVNMTILVGSGHENYGETGMAHLLEHMVF
                     KGSPRHPDIPQELTAHGSRPNGTTSWDRTNYFETFNATEENLRWALDLEADRMVNSFI
                     AKKDLDSEMTVVRNEFEAGENQPISVLFKRLLSVAYDWHNYANTPIGARSDIEGVPIE
                     RLQAFYRTYYQPDNAVLLVAGKIDEAATLKMVQEYFGPIPRPTRVLPALHTSEPTQDG
                     ERRVSLRRVGDVQATVVAYHAPAGSHEDFPALQVLGQIMADAPAGRLYKEIVEKGKAA
                     SVFPVPLQLREPGIFAFGAQVRAEQSVDTALDLIVANLEGPAAKPITDEEVERARTAI
                     LKQIELSLNNSERIGLELSNWAGMGDWRLLFLNRDRLRAVKTADVQRVWRLYFKPSNR
                     TAGVFYPTKTPDRAEIPATPDVVALVNNYKGDAAKEEGEAFDATPANIEARTKRVKLP
                     GGLDLAMLPKKTRGGAVFAALSLRFGDEASLRNMGNVPPMTTSMLMRGTKKHTRQQLS
                     DEFDKLKARISFNNWGSGQYTSIETTRENLPAVLTLLTEVLREPAFDPKELEQLRTEW
                     LASMEQQRSDPSALAFTAFQKHLKPYRADDIRYVESIDESIATTKAVTRDQLVKFHQA
                     FFGAQPAQMAVVGDFDAEQIQKQVATLLGDWKNARPFKRVASEYFEVPAKEVIIETPD
                     KAQAFFVAGMNLALRDDDPDYPALVIGNYLLGGGFLNSRLMTRIRGKDGLSYGVGSQL
                     QGDNFDKSGLFLTYAIYAPQNLAKLQQAFNEEIARVLNEDFTREEVDAAKSGWLQGRG
                     VSRAQDNELVGSLAHGMFVGRTLAWDAEFDKKVMAVDPAQIRAAFQRHLVPAKFTVVK
                     AGDFKGAAK"
     gene            8812..>10499
                     /locus_tag="VIV63_05095"
     CDS             8812..>10499
                     /locus_tag="VIV63_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HEY6124005.1"
                     /translation="MLTKFAMRGALFSALFLLASCGGGGGGGGAAPGPGPAVPAVLTV
                     VVVTQPSGGEATTPFATQPVVHLRTDGTTNTGNNVTVVTASIAPGTGTAGAALTGTAT
                     ATVVAGVATFANLGVNTPGTGYQLRFSAVDYGNATSAAFTITAPPPTPGAIIPPSTPP
                     ASQVSFAIDSTQDVRAISRFIYGMNGWDPDVRPANLTLSRSGGNRMTAYNWENNASNA
                     GSDWFNQNDDFLGGGNTPNGAVGPGITDARTAGAGIVVTVPTIGYVAADKNGGGDVNV
                     TPNYLAVRFHQSRPRKGSAFVMTPDTTDAFVYQDEYVNFLNQTYPGALASTTAPVFLS
                     LDNEPDLWHSTHARLRGDAVGQNGTNVTYAEIVQRTVDYAAAAKDVNSSVQIFGPANY
                     GWQGFLRLQNAADHQDRDFLNFYLAQMAQAGTTAGRRLLDVLDVHWYPEARGACSTNP
                     MDGCRVSDGGTGANESAVIAARKQAPRSLWDPAYQETSWILDTLPNGDKPIRLLPRLK
                     AKIAANYPDTKLAITEYNYGGGNHISGGIAQADVLGVFGREGLYAATLWRLIND"
BASE COUNT         1896 a         3446 c         3343 g         1814 t
ORIGIN      
        1 tgatcgtcat tgccatcgtg ggcatcctgg cctccatcgc ctatccggcg tactcccggt
       61 acatcgtgcg gacgcaccgc acggcggcca aggcgtgcat ggcggaagtc gcgcagttca
      121 tggagcggta ctacacgacc aacatgtcgt atgtggatgc gaatccggtc gcggcctgcg
      181 agtccgaagc cggcctcgac cagctctacg acatccgggt ggacgacatc gcggcgcgtg
      241 cgtacacggt gaccgccgag ccgatcggca cgcaggccac gcgcgacgcg cagtgcggaa
      301 cgctgacgct cgacgaggcc gcgacgcgcg gcaagtcggg ttcggccgac ttcgagtact
      361 gctggtcgcg ctagttcttc agtttgtagc cggtgcggaa catccagtgg accacaccca
      421 ggcatagcag caggaagccg agcgtcatgc cgaagctcag ggccacgttg acgtccgact
      481 tgccgtagaa gctccagcgg aagccgttca ccaggtagac caccgggttg aacagcgtga
      541 tcttctgcca gatgggcggc agcatcgaga tcgagtagaa gctgccgccg aggaaggtca
      601 gcggcgtcac gaccatcatg ggcacgatct ggatcttctc ccagctgtcg gcccagatgc
      661 cgatcaggaa gccgagcagc gcgaacgtga ctgacgtgag caccaggaac gcgatcatcc
      721 agaagggatg ctcgatggtg aaaggcacga acaggcgcgc ggtcgcgagg atgaccagcc
      781 ccaggatcac cgacttggtg gccgcggcgc ccgtataacc caacaaggtc tcggcaagcc
      841 ccacgggcgc cgacaacagc tcatagatgg tgccggcgaa cttcggcatg tagatgccga
      901 aggagccgtt ggagacgctt tccatgagca ccgtgagcat gatgagcccg ggcacgatga
      961 acgcgccgta actcacgccg tcgatgctca tcatgcgcga gccgatcgcc gagccgaaca
     1021 ccacgaagta gagcgacgtg gtcatgaccg gcgtggcgat gctctggaac aacgtgcgcc
     1081 aggtacgcga catctcgaaa cggtagatag cctggacggc gtggaagttc atgggcgtga
     1141 tctcacgagg ttcacgaaga tgtcttcgag ggagctctcg gccgtgtgca gatccttgaa
     1201 ctcgatgccg gtctcgttga gcttgcgcag cagcccggcg atgccggtct cgctggcctg
     1261 cgtgtcgaag ctgtagacca gctcggtgcc ttccttggcc agctcgagcg gcagccccgc
     1321 gagccccggc ggaatggcgg ccaggggtgc ctgcagttgc agcgtgagct gtttcttgcc
     1381 gagtttgcgc atgagcgcgg ttttttcctc caccacgatc agcgagccct tgctgatgac
     1441 gccgatgcgg tcggccatct cctcggcttc ttcgatgtag tgggtagtga ggatgatggt
     1501 gacgccgctg gcgcgcagct tgcggaccat ttcccacatg tcgcggcgca gctccacgtc
     1561 cacgcccgcc gacggttcgt cgaggaacag gatgttgggc tcgtgggaca gcgccttggc
     1621 gatcatcacg cggcgcttca tgccgcccga gagcgtcatg atcttcgcgt ccttcttgtc
     1681 ccatagcgaa aggtcgcgca gcaccttctc gatgatggcg gggttgcgcg gcttgccgaa
     1741 caggccgcga ctgaacgtga gcgtgttcca caccgtctcg aacatgtcgg tggtgagttc
     1801 ctgcggcacg aggccgatcg tggcccgggc ggcgcggaag tcgcgcacgg tgtcgtggcc
     1861 gtcggcggtg acggtgcccg tggacgccgt gacgatgccg cagatgatgt tgatgagcgt
     1921 ggtcttgccg gcgccgttcg gacccagcag cgcgaatatt tcgccgcgcc gtatttccag
     1981 gtcgacgttc ttgagcgcgt ggaagccgga ggcgtaggtc ttggaaagac ccgaaactgt
     2041 taacacggac atgggcggct aagttagcga tgcacaggcc gcgactcaat ggtccattgg
     2101 gggtagggcg gctagacttc gtgaatctgg attaagcgac acttgctacc cgagggccct
     2161 atgcaacgtt cctggatggc ctacatttcc gcgggccttg gcggcgccgc gctgctcggc
     2221 gtcatcagtt ttcattttcc gacgctgctc accagccggg acttccgcgc cgtctacacc
     2281 gaggatttcg cgcgcaacct gctgttgtta gggctggtgc tgtccttcac gctcggcacg
     2341 gtggcggtcc tccgccgccg cagcgtcagg gtggcgctga cgggtgtgtg tctcgcggcg
     2401 ctggcggtgt tcctcggcgg ggccaccatc gaactcgacc ccatccggag caccgcgttt
     2461 tcgctggggc tcgactggtt cgtgatctcg ctgttcttct cggcgctggt gttcattccg
     2521 ctcgagcgca tgctggcggt acgcacgttg tcgccactgc ggcccgaatg gcgcaccgac
     2581 ctcgtgtatt tcttcatcag ccacgtgctg gtccagttca tcctcatcgc cgtcacggct
     2641 tccaccacga cggtcgcggc gctggtgctg ttccccgccg tgaagacgtg gatccaggcg
     2701 ttgccggtgt ggttgcagtt cctcatggcc gtgttcctcg ccgatctgtt ccaggccggt
     2761 ttgcaccgca tctaccacaa ggtgccgtgg ctctggcgct tccacgccgt gcatcactcc
     2821 agccgaaatc tcgactggct ggccgggtcg cgcgtgcaca tcgtggagat cgtgctgact
     2881 cgcagcgccg tcctgttgcc gctggtggta ctcggtttct cgcagccggc ggtgaatgcc
     2941 tacgtcatcc tggtcggcat ccaggccgtg ctggcgcacg ccaacatgcg cacccacttc
     3001 ggctggctgg aatacctgct cgtgacgccg cgttatcacc actggcacca tgcgcgccat
     3061 gtcgactaca tggacgtgaa ctacgccatc catctgccgc tggtcgacat gctcatgggc
     3121 acgttcaagc gtccgccgcc cgacgtctgg cccgatgaat acggcgtcat gaagctcgag
     3181 accgtgccgc gcggcatctg ggctcagacg ctgatgccga tggggccgaa gaagaccttc
     3241 gacgatcacg tgggcgcgga gacgccgtcc acctgatggc cgtccgcgcg tttctcgttg
     3301 cgctcgcgtg cctgtgcctg ccgacggccg cgggcgctgc cggcggcgcg gacatcgtcg
     3361 tcgccgcgcg cggccaggtc ggcgtcaccg tggtttacga cccggcgtac cggtccctgg
     3421 gatatcccgg cggcgatgtg cccgcggatc gcggcgtatg caccgatgtc gtggttcgcg
     3481 cgctgcgcac ggcgcgagcc atggacctgc agcgccgggt gcacgaggac atgaaggccc
     3541 actgggacgc gtatccgcat catcgccggt ggggtctgtc gaagccggat cccaacatcg
     3601 atcaccggcg cgtgccgaat ctcatgacgt atttcaagcg cgcgggctac gaaacgcggc
     3661 tcacgcgaac cgccgccgac tatctacccg gcgacatcgt cgcctgggat ctgggacgcg
     3721 gcatcctgca catcggcatc gtgagcgacg tcaaatcggt gaccggcacg ccgctcgtgg
     3781 tacataacat cggttccggc gcgcgggaag aggacatcct gtttcgattc acgataatcg
     3841 ggcattaccg cctgccgccg cccccggtcg ccgccgccag ggcatctgta gcctcacgct
     3901 gacgccattc gagcatcgat tcacggtacg cttcgcccgc agacatggtc gccacgcccc
     3961 gcattgccaa acaagccgaa gtccacggcg aatcgctgaa ttcaccgcga cagttcttcg
     4021 gtgttttcaa gtacagcaaa cgagcgctcg agctcctctg gaccacgagc cggccgctgg
     4081 cgttcttcct gggattcatg accgtccttg ccggcgtgct gccctcgggc gtggcctggg
     4141 tgggcgcccg catcgtcgac agcgtgctcg aggcaacgcg gacgcattcc gcgggatccg
     4201 acgtcaacat cacgcccgtg ctcatgtggg tgctggccga ggcactgatc ctcgtggtca
     4261 tcacgggtgc gcaccggggc atcacgttct gcagcgcgct gctcaaggcc aagctctcga
     4321 accgggtcaa cgtgctcatc ctcgagaagg cgctgacact cgagctcacg catttcgaag
     4381 actcggagtt ctacgacaag ctcacgcgcg cgcggcgcga agcctcgagc cggccgctgt
     4441 cgctggtcat gcgcacgtcg ggtctcttcc agaacgccat cgccatcgcc agcttctcgg
     4501 tgctgctcgc cggtttctcg ccctgggccg tggtcgttct catcttcggc ggtctgcccg
     4561 ccttcttcgc cgagacgaag ttctccggtg acgcattccg cctgttccgc tggcgcgcgc
     4621 ccgagacgcg catgcagagt tatctcgaaa cagcgctcgc gcgcgaagat catgccaagg
     4681 aagtcaaact gttcgacctc ggcaaactct tcctcggccg ctacaaggcg atctacgaag
     4741 ggttgtacga gaaggaccgc aatctcacgg tccgccgcga cgcgtggggc ttcgcgttca
     4801 cgctggtgag catcgcggcg ctgtacagcg cctacgcgtg gtgcgcggtg gaagcggtgc
     4861 gcggagtcat cacgctcggc gagatgacca tgtacatcat gctgttccgc cagggccagg
     4921 cggccgtcac cgccagcctc ggcgccatcg gtggcatgta cgaggacaac ctctatctct
     4981 cgacgctgta cgaatacctc gatcagccgg tggaggagag caagggaacc gcggtgagcg
     5041 gccccgaccc gacggcgggc gtgcagttcg aggacgtcga gttccgctat ccgggctccg
     5101 accaggtggc gatcaccggc gtcaatctgc agatcagact cggcgaaagc ctggcgctcg
     5161 tgggagaaaa cgggtcgggc aagaccacgc tgatcaagct gctcacgcgc ctgtacaagc
     5221 ccacgcatgg ccgcgtgctg ctcgatggcc gcgacctgca ggaatgggac accacggtgc
     5281 tgcgccggcg cgtcggcgtg atcttccagg atttcgtgcg ttatcagctc atggtgggcg
     5341 agaacatcgg cgccggtgac gtgcgcgcct tcgaagacga agctcgctgg aaggaagccg
     5401 ccaactccgg caaggccgcg ccgttcatcg acgacatgcc gctcaggttc cacacgcagc
     5461 tcggcaagtg gttcaaggac ggccgggaac tctccggcgg ccagtggcag aaaatcgcgt
     5521 tgtcgcgcgc tttcatgcgc agcgacgccg acatcctggt gctcgacgag cccactgccg
     5581 ccatggacgc ggccgccgaa gcggaagtct tcgaacactt ccgttcgctc atgggcaatc
     5641 gaatcgccat cgtcatttcg caccggttct ccaccgtgcg catggccgac cagatcgtgg
     5701 tgctcgacca ggggcacatc gtcgagcgcg gcaaccacga actgctcatg aagcaggacg
     5761 gcatctactc caaactattc acgctgcagg cgcgcgggta taggtagacc cggcgtcgtt
     5821 cacggcgcgt tcagatacaa tcgcgcacct ttccgtacct cactagctag agcagggagt
     5881 gcccacatgc ctacgaacca tcgtgtcctg gcgtcgttga cgttggtcgt ggcgctcgcc
     5941 accccggcct gggccgcgaa aaaaccgaag gctgctcccg cgccgccgcc cgtggcggcc
     6001 cccgccgcgc cggaacgcgt caccacggtc gaaggcatca ccgaataccg gctggcgaac
     6061 ggcctgcgcg tgctcatgtt cccggaccag tcgaagcaga ccatcacggt gaacatgacc
     6121 atcctggtcg gctcgggcca cgaaaactat ggcgagaccg gcatggcgca tctgctcgaa
     6181 cacatggtgt tcaagggctc gccgcgccac cccgacatcc cgcaggaact cacggcgcac
     6241 ggctcgcggc cgaacggcac cacgtcctgg gaccgcacca actacttcga gacgttcaac
     6301 gccaccgagg aaaacctgcg ctgggcgctc gatctcgagg ccgaccgcat ggtcaattcc
     6361 ttcattgcga aaaaggatct cgacagcgag atgacggtgg tgcgcaacga attcgaggcc
     6421 ggcgaaaacc agcccatcag cgtgttgttc aagcggctgc tgtcggtggc ctatgactgg
     6481 cacaactacg ccaacacacc catcggcgcg cgctcggaca tcgaaggcgt acccatcgag
     6541 cggctgcagg cgttctatcg cacctattac cagcccgaca acgccgtgct gctggtggcc
     6601 gggaagatag acgaggcggc cacgctcaag atggtccagg aatatttcgg ccccattccg
     6661 cgacccacgc gcgtgttgcc cgcgctgcac accagcgaac ccacgcagga cggcgagcgg
     6721 cgcgtgtcgt tgcgccgcgt gggcgacgtg caggcgacgg tcgtcgcgta tcacgcgccc
     6781 gcgggctccc acgaagattt cccggcgctc caggtgctcg ggcagatcat ggccgacgct
     6841 cccgcgggac ggctgtacaa agagatcgtg gagaaaggca aggcggcgtc tgtctttccg
     6901 gtcccgctgc agttgcgcga gccgggcatc ttcgccttcg gcgcccaggt gcgggcagag
     6961 cagtcggtcg ataccgcgct cgatctgatc gtcgcgaacc tcgaaggtcc ggcggcgaaa
     7021 cccatcaccg acgaggaagt cgaacgcgcg cgcacggcga tcctcaaaca gatcgagctc
     7081 agcctgaaca actcggagcg catcggcctc gagctgtcga actgggcggg catgggcgac
     7141 tggcgcctgt tgttcctgaa tcgcgaccgg ctgcgcgccg tcaagaccgc cgacgtgcaa
     7201 cgcgtctggc ggctctattt caagccgtcg aaccgcacgg ccggcgtttt ctatcccacc
     7261 aagacgcccg atcgagcgga gattccggcc acgcccgatg tcgtcgcgct ggtcaacaac
     7321 tacaagggtg atgcagccaa ggaagagggc gaggcgttcg acgccacgcc ggccaacatc
     7381 gaggcgcgta cgaagcgcgt gaaactgccg ggcggcctgg atctcgccat gctgcccaag
     7441 aagacgcgcg gtggcgcggt gttcgccgcg ttgtcgctgc gtttcggcga tgaggcgagc
     7501 ttgcggaaca tgggcaacgt gccgccgatg accacgtcca tgttgatgcg cggcacgaag
     7561 aagcacacgc gccagcaact ctccgatgag ttcgacaagc tcaaggcgcg catcagcttc
     7621 aacaactggg gctcgggcca atacacctcc atcgaaacca cgcgcgagaa tcttcccgcg
     7681 gtgctcacgc tgctcaccga ggtcctgcgc gagccggcct tcgatccgaa ggagctggag
     7741 cagttgcgca ccgagtggct cgccagcatg gaacagcagc gcagcgatcc gtcggcgctg
     7801 gcgttcacgg ctttccagaa acatctgaag ccgtatcggg ccgacgacat ccgctacgtg
     7861 gagtccatcg acgagagcat cgccaccacg aaggcggtga cgcgcgatca gctggtgaag
     7921 ttccaccagg ccttcttcgg cgcgcagccg gcgcaaatgg ccgtggtcgg cgacttcgac
     7981 gccgaacaga tccagaagca ggtcgcgact ttgttaggcg actggaaaaa cgccaggccg
     8041 ttcaagcgcg tggcgagcga gtacttcgaa gtgcccgcga aggaagtgat catcgagacg
     8101 ccggacaagg cgcaggcgtt cttcgtcgcc ggcatgaacc tcgcgctgcg cgacgacgat
     8161 cccgactatc cggcgctggt gatcggcaac tatctgctgg gaggcggctt cctcaactca
     8221 cgtctcatga cccgcatccg cggcaaggat ggcctgagct acggtgtggg ttcgcagctt
     8281 cagggcgaca acttcgacaa gtccgggttg ttcctcacat acgccatcta cgcgccgcag
     8341 aatctcgcca agctgcagca ggcgttcaac gaagagatcg cccgtgtgct caacgaggat
     8401 ttcaccaggg aagaagtgga cgccgccaag tcgggctggc tgcaggggcg cggcgtctca
     8461 cgcgcgcaag acaacgagct ggtgggatcg ctcgcgcacg ggatgttcgt cggccgcacg
     8521 ctcgcctggg atgcggagtt cgataagaag gtgatggcgg tcgatcccgc gcagattcgc
     8581 gccgcgttcc agcgtcacct ggtgccggcg aagttcaccg tcgtgaaggc cggcgacttc
     8641 aagggagccg ccaagtaaat ggggacattc ctgatttgct agcaaaaggg gacgcacctc
     8701 aggggccgcg cccccgataa atgtgacgcc tatcgcgtct cggaccgccg acacattgtg
     8761 atgcgcgcga agccgcggtt atggttgcct gaggcataac cgggggcttg aatgctgacg
     8821 aaattcgcca tgcgcggtgc gctgttctca gcgctgttct tgttagcgtc atgcggcggt
     8881 ggtggcggtg gtggcggcgc ggcgcccggt cccgggcccg ccgtacctgc ggtactcacc
     8941 gtggtcgtcg tcacccagcc ctcgggcggc gaggccacga cgccattcgc cacccagccc
     9001 gtcgtgcatc tgcgaaccga cggcaccacc aacaccggca acaacgtcac ggtggtcacg
     9061 gcctccattg ccccgggcac cggcactgcc ggcgcggcac tcaccggaac cgcgaccgcc
     9121 acggtggtcg ccggcgtcgc cacgtttgca aatctcggcg tcaacacccc gggaacgggc
     9181 tatcaactgc gcttctcggc tgtcgactac ggcaatgcca cgagcgcggc cttcaccatc
     9241 accgcaccac cgcccacccc cggtgcgatc atcccgccat ccacgccccc cgcgagccag
     9301 gtcagcttcg ccatcgacag cacgcaggac gtgcgcgcga tctcccgctt catctacggc
     9361 atgaacggct gggatccgga cgtgcgcccc gccaatctca cgttgtcgcg ctcaggcggc
     9421 aatcgcatga cggcttacaa ctgggagaac aacgccagca acgccggcag cgactggttc
     9481 aaccagaacg acgatttcct gggcggtggc aacacgccga atggcgctgt aggtccgggc
     9541 atcaccgacg cgcggaccgc cggggcgggt atcgttgtca cggtgccgac catcggctac
     9601 gtggcggccg acaagaacgg cggcggtgac gtcaacgtca ctcctaacta tctcgccgtg
     9661 cgattccacc agagccggcc gcgcaagggc agcgcattcg tgatgacccc ggataccacc
     9721 gatgcgttcg tctaccagga cgagtacgtc aacttcctga accagaccta tccgggcgcg
     9781 ctcgcatcca ccacggcgcc tgtctttctc agcctcgaca atgagcccga cctctggcac
     9841 tccacgcacg cgcggttacg cggcgatgcg gtggggcaga acggcacgaa cgtgacctac
     9901 gccgagatcg tgcagagaac cgtcgactac gcggccgccg cgaaggatgt gaattcctcg
     9961 gtacagatat tcggaccggc caattacggc tggcagggct tcctgcgcct gcagaacgcc
    10021 gcggaccacc aggaccgcga cttcctgaac ttctatctcg cgcagatggc gcaggctggc
    10081 accacagcgg gacggcggct gctcgacgta ctcgacgtgc attggtatcc ggaagcgcgc
    10141 ggcgcctgca gcaccaatcc catggatggc tgccgagtca gtgacggcgg cacgggcgcc
    10201 aacgaaagcg cggtcatcgc ggcgcgcaag caggcgccgc gtagtctctg ggaccctgcc
    10261 taccaggaga ccagctggat tctcgacacg ctaccgaacg gtgacaagcc catcaggctg
    10321 ctgccgcggc tcaaggcaaa aattgcggcc aactacccgg acacgaagct ggcgatcacc
    10381 gaatacaact acggtggcgg caaccacatt tccggcggca ttgcccaggc cgacgtgctg
    10441 ggtgtcttcg gacgcgaagg gttgtacgcc gcgactctat ggcggctgat caacgacaa
//
LOCUS       DATDEU010000208         8163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_36918, whole genome shotgun sequence.
ACCESSION   DATDEU010000208 DATDEU010000000
VERSION     DATDEU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36918"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VIV63_05100"
     CDS             complement(<1..390)
                     /locus_tag="VIV63_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6124006.1"
                     /translation="MAILPELETPRLILRRLEFDDAPFLVKLLNEPSFLENIGDRGVR
                     TIEDAHRYVREGPMAMYDRYGFGLWHTASREGVPMGMCGLLKRDILPDVDVGYAFLPQ
                     FWGQGFAYEATAATLRHGARKFGLRRVV"
     gene            complement(374..787)
                     /locus_tag="VIV63_05105"
     CDS             complement(374..787)
                     /locus_tag="VIV63_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6124007.1"
                     /translation="MNQDIQGGCMCGAVRYSATTNPANSMVCHCQSCRRSAASPVVAW
                     VTFAKAEFKFTRGTPREFESSAKVKRRFCGDCGSPLTYEHIDTKDFVDVTTCSLDAPW
                     GFPPTHHSWLSDDIGWVKFGDGLPTFERSRFGDPS"
     gene            complement(1016..2116)
                     /locus_tag="VIV63_05110"
     CDS             complement(1016..2116)
                     /locus_tag="VIV63_05110"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY6124008.1"
                     /translation="MNSSIAILIGGVVVVLVGIVIWLRATKQKPTPVDPIESTTGSNR
                     ALTGAQPTGAVASLNWDEALRRFVAYALDDVPRESLSRTPDPTHAPVFIAVQQILERI
                     EARPEYIPRRPSLLPKLLATVNDNEATMAEMSRIIAQDPALTGNLLRIANSPVYRTSS
                     LPVDSIDRAVTLVGVQGIRSIIATALLQPVMSSASGAAFAKFPELVWEHTLYAAMAGE
                     AHATQVENAEPFVSQLIGLLYGLSAIVVFRIVRDQFALHPHLNPDPGSIARMLESWVA
                     PTAGRIAVSWELSTRVQYALESQTLAAELQMENSLGRSLKFGRVTGALIVLCRLGRIS
                     EQDARAIVVSGEARRSKVERLWDRLATGYLKP"
     gene            2238..2717
                     /locus_tag="VIV63_05115"
     CDS             2238..2717
                     /locus_tag="VIV63_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6124009.1"
                     /translation="MPTLSLRVNGKDLAADVDPNTPLLWVLRDALGLVGTKFGCGMAL
                     CGACTVLADGEPVRACATPVSSMAGKNITTIEGLAKSATQLHPLQEAWIEHDVPQCGY
                     CQAGQLMSASALLARTPQPTDAEIDSAMAGNLCRCGTYQRIRAAIHTAAGKMAKGKA"
     gene            2717..4984
                     /locus_tag="VIV63_05120"
     CDS             2717..4984
                     /locus_tag="VIV63_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6124010.1"
                     /translation="MNARLNPAAAADEALRELMRDVELGASSLPMRAVKVDRRTFLKL
                     TGMAGGGLTLGFLVGGDAGAAAAKSGTGDFVPNAFVRISSKGSILIYNKGPEIGQGIK
                     TAFPLIIAEELDALWTDVVVEQAPVDPAVYGRQSAGGSRSIPDSWDQLRKAGAVARSM
                     LVSAAAAAWKVPVEECTARDSAVWNGKKKLTYGALAAKAAALPVPDPATVKLKERKDY
                     RLLGKSYTGVDNRKVVTGAPLFGIDQQLPGLKFAVYEKCPATGGKVRSANLDEIKKLP
                     GVRDAFVIEGNGKPTEVMPGVAIVADSTWAAFEARKQLRVDWDESAASKDSWSAITKR
                     AAELGKQAAGETTTLSVGATGDALKGAAKSVEGFYTYPFVSHAPLEPQNCTAWNHDGI
                     LELWSPTQTADRSLQTLAGALGVPVEKIKINQTRVGGGFGRRLMNDYACEAGAIAQRF
                     AGPVKLMWTREQDMAHDFYRPGGFHSLAGGVDASGKLVAWRNHFISFTTDGKNPVSGG
                     NLPDQEFPAQNVPNYQLTQTLLPLATPCGPWRAPRSCSVAWVIQSFLHELSAAANRDH
                     RDFLLEVMGEPRLLPGGLNTGRAADVIKLVTEKAGWGRKLPAGHGMGLAFHFSHAGHF
                     AEIAEVSVDANKRIRLHRMVVVGDIGPIVNMSGANNQCEGGVIDGFSTMLGLEITMEN
                     GRVEQSNFHQYPILRIADQPKVEVFFIQSDNPPTGVGEPALPPVAPAICNAIFAATGH
                     RVRTLPLSKEGFSVA"
     gene            5108..6496
                     /locus_tag="VIV63_05125"
     CDS             5108..6496
                     /locus_tag="VIV63_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY6124011.1"
                     /translation="MEARLVFHDDAHTRVAATIMETLVRAGIAVAPDGACAAIPTLVF
                     FRAADDELLVKLDGLRDAGCERILAIAMHERPEQAQVWRILDSGASDLLPWSGGDDDA
                     RTLAARLMRWRQVDELLQSSLVAGSMIGKSGRWQSTLRQIVEVARFTPASALVLGESG
                     TGKELVARLIHALDPRPGKGELIVVDCTTVVPELSGSEFFGHERGAFTGAAGTRDGAF
                     ALARGGTLFLDEVGELPLPLQAQLLRAVQEQTFKRVGGNTWFKTDFRLVCATNRDLLA
                     AVQRGEFRHDLYHRIANWVFTLPPLRERTEDVVPLARHFANQRQTGNKQVDLDAAVEE
                     CLLRRDYPGNVRDLRQLVMRIMDRHVGEGAITAGDLPPAERPQDAGNFDWASDENFLR
                     SIQLALSQGIGMKEIGRAAEEGAMKLALREADGNLQRAANRLGVTDRALQLRRASARQ
                     SAQPQQPTGAAS"
     gene            complement(6463..7323)
                     /locus_tag="VIV63_05130"
     CDS             complement(6463..7323)
                     /locus_tag="VIV63_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6124012.1"
                     /translation="MIIDCHCHAGKGDGLTGPWDTRAPLKAYLQRASQAGIRRTVLFA
                     AFTSDYESANREVARIVRSRPGQFYGFAFVNAVRDAGRVRRLVEVAVRQYGFVGIKVH
                     RHEGRITREICDTARAFGLPVLYDVMGEVSACELLAREYPDVPFIIPHLASFAGDWRA
                     HLALIDQLVRHRNMHTDTSGVQRFDLLAEAAQRAGARKILFGTDGPWLHPAVELAKVR
                     ALELSHTEESLILAGNFLRLIEGVVTQPRFTPRRLRTVTAAAAWQGETRDPWLRQTTD
                     QEAAPVGCCG"
     gene            complement(7327..>8163)
                     /locus_tag="VIV63_05135"
     CDS             complement(7327..>8163)
                     /locus_tag="VIV63_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY6124013.1"
                     /translation="LNESGASTAKILLFYAAHQMLFDLEEMVKAGGIKKTADLRKKAN
                     AEWAKFTCKPHFDWLFKFTESAGPLKVEMSDALNTTMANIVDEKSSTPNASRLILRLS
                     FEYLASLAWQSGLRHPSRKGVWYGSTYCQTGGAPAPISGACHVAGGTESGCGQNQSRI
                     IWTKDPLGHRSVRGSALSYVTYMTLLAQARLVNKGMSARIEALLKRACGFIQRVVPGM
                     RANKCGLTNTLFHDAAIIEHDKVRYAVAYMFKSKSLADPLKGKVLDLMREIDKIIVAA
                     NP"
BASE COUNT         1458 a         2633 c         2667 g         1405 t
ORIGIN      
        1 gacgacacgc cggagtccga atttcctggc accgtggcgc agcgtcgcgg cggtggcttc
       61 atacgcgaat ccctggcccc agaactgcgg caggaacgca taacccacgt cgacatccgg
      121 caggatgtcg cgcttgagca gcccgcacat gcccatcggc acgccctcgc gcgacgccgt
      181 gtgccacagg ccgaagccgt accggtcgta catcgccatc gggccttcgc gcacgtagcg
      241 atgcgcatct tcgatagtgc gcacgccgcg atcgccgatg ttctcgagga acgacggctc
      301 gttgagcagc ttcacgagga acggagcgtc gtcgaactcg agacgccgca atatcaggcg
      361 cggggtttcg agctcaggaa ggatcgccaa accgtgatcg ctcgaatgtc ggcaggccat
      421 cgccgaattt cacccagccg atgtcgtcgc tgagccagga gtgatgtgtc ggcgggaatc
      481 cccacggcgc atccaggctg cacgtggtca cgtcgacgaa atctttggtg tcgatgtgtt
      541 cgtacgtcag cggcgagccg cagtcgccgc agaaacggcg cttcaccttc gccgaggatt
      601 cgaactcgcg cggcgtgccg cgcgtgaact tgaattccgc cttggcgaac gtgacccagg
      661 caaccaccgg cgatgcggcc gagcggcgac acgactggca gtgacagacc atgctgtttg
      721 ccggattggt agtagcggaa taacgcacgg cgccgcacat gcagccgccc tgaatgtcct
      781 ggttcaaatc cttttcccct gttttgaagc cgcagcttac cgtgagtgat gcctgcaaaa
      841 aaaagagccc cgcctgagcg gggctctagt cgagcggaaa cgggggtctt gttccgctgc
      901 tctggtctgt ggaaaccagt agggttccgg tgtccacgaa tactctgtgt cacgaggggg
      961 cgttttctcc caccctgcgg acacctttat tgagcgtaat gcgaaggatt gccggttacg
     1021 gcttcaggta accggtggca aggcgatccc agaggcgctc gaccttcgac cggcgggcct
     1081 cgccagacac gacgatggcc ctggcgtcct gttcggagat ccggccaagt cggcagagca
     1141 ctatcaacgc accggtaacc cgcccgaatt tcagcgatcg gcccagcgaa ttctccatct
     1201 gcagttccgc cgccagtgtc tggctctcga gcgcgtactg gacccgggtc gacaactccc
     1261 agctcacggc gatgcgtccg gcggtcggcg ccacccagct ttccaacatg cgggcgatgc
     1321 tgcccggatc gggattgagg tggggatgga gcgcgaactg gtcgcgcacg atgcggaaca
     1381 ccacgattgc ggaaagtccg tatagcaatc cgatgagctg cgacacaaag ggctcggcat
     1441 tctcgacctg ggtcgcgtgc gcttcacccg ccatcgcggc atacaacgtg tgctcccaga
     1501 ccagttccgg gaatttggcg aaggccgcgc cggaagccga ggacatgacc ggctgaagca
     1561 atgccgttgc gatgatggat cggattccct gcacgcccac cagggtcacg gcccggtcga
     1621 tgctgtccac gggcaggctg ctggtgcggt agacagggct gttcgcgatc cgcagcaagt
     1681 tgccggtaag cgccggatcc tgcgcgatga tgcgcgacat ctccgccatg gtcgcttcgt
     1741 tgtcgttgac cgtcgccagc agtttcggga gcagcgacgg ccgtcgcgga atgtattcgg
     1801 gccgcgcctc gatgcgctcg aggatttgct gcaccgctat gaaaaccggt gcgtgcgtgg
     1861 gatccggcgt ccggctcaac gactctcgag gcacgtcatc gagcgcgtaa gcgacgaacc
     1921 tgcgcaacgc ttcatcccag ttcagcgacg ccacggcgcc cgtgggttgc gcacccgtca
     1981 gagcgcgatt cgagcccgtg gtcgactcga tgggatcgac tggagtcggt ttttgtttgg
     2041 tagcgcgaag ccagatgacg atgccgacca gcaccaccac gaccccgccg atcaggatgg
     2101 cgattgacga attcatgccg gaaggctacc cggtcgcgcc ccccacccgc cagttgcgcc
     2161 ttttgtctcg ttgccgcggc acccgcacgg cgaatacgat cgcccattct ccggctcacc
     2221 tctaaggaag tctccgcatg cccaccctca gcttgcgagt gaatggcaag gacctggccg
     2281 ccgacgtcga tccgaacacg ccgctgttgt gggtgttgcg cgatgcgctg gggctcgtcg
     2341 gcacgaagtt cggctgtggc atggcgctgt gcggcgcgtg cacggtgctc gccgacggcg
     2401 aaccggtccg cgcctgcgcg acgccggtgt cgagcatggc ggggaagaac atcacgacca
     2461 tcgaaggtct cgcgaagtcc gcgacgcaac tgcatccgct gcaggaagcg tggatcgaac
     2521 acgatgtccc gcaatgtggt tactgccagg cgggccagct catgtcggct tcggcactgc
     2581 tcgcgcgcac gccgcaaccg accgatgccg agatcgactc ggcgatggcg ggcaatctct
     2641 gccgctgcgg cacgtaccag cgcatccgcg cagccatcca taccgctgcc ggcaagatgg
     2701 cgaaggggaa ggcctgatga acgctcgcct gaatcccgcg gccgcagccg acgaagcgtt
     2761 gcgcgaactc atgcgcgatg tagagctcgg tgcatcatcg ctgccgatgc gcgcggtcaa
     2821 agtcgaccgg cgcacctttc tcaaactcac cggcatggcg ggcggtggac tcacgctcgg
     2881 cttcctcgtc ggcggtgatg ccggcgccgc cgccgcgaaa agcggcaccg gcgatttcgt
     2941 gcccaacgcc ttcgtgcgca tttcgagcaa gggctcgatc ctcatctaca acaagggtcc
     3001 ggaaatcggc cagggcatca agaccgcgtt tccgctgatc atcgccgagg agctggacgc
     3061 gctctggacg gacgtcgtcg tcgagcaggc gccggtcgat ccggcggtgt acggacggca
     3121 gagcgccggc ggttcgcgct ccatccccga cagctgggat cagcttcgca aggcgggcgc
     3181 ggtggcgcgc tccatgctgg tgtcggccgc ggccgccgcg tggaaggtgc cggtcgaaga
     3241 atgcaccgct cgcgacagcg cggtctggaa cggcaagaag aaactcacgt atggcgcgct
     3301 ggccgcgaaa gccgcggcgc tgccggtgcc cgatccggcc acggtcaagc tcaaggagcg
     3361 caaggactac cggttgttag gcaagtcgta caccggcgtc gacaatcgca aggtcgtcac
     3421 gggcgcgccg ctgttcggta tcgaccagca gcttccgggc ctcaagttcg cggtgtacga
     3481 gaaatgcccg gcgacgggtg gcaaggtccg ttcggcgaat ctcgacgaga tcaagaagct
     3541 gccgggcgtg cgcgacgcgt tcgtcatcga aggaaacggc aaacccaccg aggtgatgcc
     3601 cggcgtcgcc atcgtcgcgg attccacgtg ggccgcgttc gaggcgcgca agcagttgcg
     3661 cgtggattgg gacgaatccg ccgcgtcgaa ggatagctgg agcgccatca cgaaacgcgc
     3721 cgcggaactc ggcaagcagg cggccggtga gacgacgacg ctcagcgtcg gggccacggg
     3781 cgatgcgctc aaaggcgccg cgaagtccgt cgaaggtttt tatacctacc cgttcgtgtc
     3841 gcacgcgccg ctcgagccgc agaactgcac cgcatggaac catgacggga ttctcgagtt
     3901 gtggtcgccg acgcaaaccg cggaccgctc gctgcagaca ctcgccgggg cgctcggcgt
     3961 ccccgtcgag aagatcaaga tcaaccagac acgcgtcggc ggtggattcg gccgccggct
     4021 catgaacgac tacgcctgcg aagccggcgc catcgcgcag cgtttcgccg gccccgtgaa
     4081 gctcatgtgg acgcgcgaac aggacatggc gcacgacttc tatcgcccgg gcggattcca
     4141 ctcgctggcc ggcggcgtcg acgcgtccgg caaactcgtc gcctggcgca atcacttcat
     4201 atcgttcacc accgacggca agaacccggt gagcggcggc aacctgcccg accaggaatt
     4261 tccggcgcag aacgttccta actaccagct cacgcagacc ctgctgccgc tggcgacgcc
     4321 gtgtggtccg tggcgcgcgc cccgctcctg ctcggtcgcg tgggtcatcc agagtttcct
     4381 gcacgaactg tccgcggcgg cgaatcgcga tcatcgcgac ttcctgctcg aggtgatggg
     4441 tgagccgcga cttctgccgg gtggcctgaa tacgggacgc gcggccgacg tgatcaaact
     4501 ggttacggag aaagccggct ggggccgcaa gctgccggcg ggccatggca tgggactggc
     4561 gttccacttc agtcatgccg ggcatttcgc cgagatcgcc gaagtcagcg tggatgcgaa
     4621 caagcgtatc cggctgcatc gcatggtggt ggtcggcgac atcggcccca tcgtcaacat
     4681 gagcggcgcc aacaatcagt gcgaaggcgg cgtcatcgac gggttcagca cgatgctggg
     4741 tctcgagatc acgatggaga atggccgcgt cgagcaatcc aactttcacc agtacccgat
     4801 cctgcgcatc gcggatcagc ccaaggtcga agtgtttttc atccagagcg acaacccgcc
     4861 gacgggcgtg ggtgagccgg cattaccgcc ggtggcaccg gcgatctgca acgcaatatt
     4921 tgctgcaacc ggtcaccgcg tgcgcacgct gcctctttcg aaggaaggtt tttcggtggc
     4981 ctgagttctg caatgcacag gacttttgtc tcgtcttgac gagaaaccca ccgacccgcg
     5041 caggcaatcg ccggtacacg cgggctaatc tcgggtcgtt cgcaaggaag ttgaacaccc
     5101 gaggaacatg gaagcccgtc tcgttttcca cgacgacgcc cacacacgcg tcgccgcgac
     5161 aatcatggaa acactggtgc gcgccggcat tgccgttgcg cccgacggcg catgcgcggc
     5221 catccccacc ctcgtgttct tccgcgcggc cgacgacgaa ttgctggtga agctcgatgg
     5281 cctgcgtgac gcgggttgcg aaagaatact ggccatcgcg atgcacgagc ggccggagca
     5341 ggcgcaggtc tggcggatcc tcgattcggg tgcgtccgat ctcctgccct ggtcgggcgg
     5401 cgatgatgac gcgcgcacgc tggctgcgcg gctcatgcgc tggcgacagg tggacgaact
     5461 actgcaatcc tcgctggtgg ccggatccat gatcggcaag agcggacgct ggcagtcgac
     5521 gttgcggcag atcgtcgaag tggcgcgctt cacgcccgcg agtgcgctgg tgctcggcga
     5581 gagcggcacg ggcaaggaac tcgtcgcgcg tctgatccac gctctcgacc cgcggcctgg
     5641 caaaggcgag ctgatcgtcg tcgactgcac cacggtcgtg cccgaattgt cgggcagtga
     5701 attcttcgga catgaacgcg gggcgttcac cggcgccgcc ggcacgcgcg acggcgcctt
     5761 cgcgctggcg cgcggcggca cgttgttcct cgatgaagtg ggagaactgc cattgccgtt
     5821 gcaggcgcaa ctgctgcgcg cggttcagga gcagacgttc aagcgtgtgg gtggcaacac
     5881 ctggttcaag acggattttc gcctggtgtg cgccaccaat cgcgacctgc tggccgccgt
     5941 gcagcgcggt gaattccgcc acgacctgta tcaccgcatc gccaactggg tattcacgct
     6001 gccgccgctg cgagagcgca ccgaagatgt ggtcccgctg gcgcggcatt tcgccaatca
     6061 gcggcagacc ggaaacaaac aggtcgatct cgatgccgcg gtggaagaat gcctgctgcg
     6121 ccgggactat ccgggcaacg tgcgcgatct tcgccagctc gtcatgcgca tcatggaccg
     6181 gcacgtgggt gaaggcgcca tcaccgctgg cgatctgccg cccgccgaaa ggccgcagga
     6241 cgcaggaaac ttcgactggg cgagcgacga aaacttcctg cggtccatcc agcttgcgct
     6301 gtcgcagggt atcggcatga aggagatcgg gcgcgccgcc gaagaaggcg cgatgaaact
     6361 cgcgctgcgc gaagcggatg gcaatctgca acgtgcggcg aaccggctcg gcgttacgga
     6421 tcgcgcactg caattgcggc gcgccagcgc gcgacagtcg gctcagccgc agcagcccac
     6481 cggcgccgct tcctgatcgg tagtttggcg cagccaggga tcgcgggttt cgccctgcca
     6541 cgcggcggct gccgtcacgg tccgcaacct gcgtggcgtg aaacgcggct gcgtgaccac
     6601 gccctcgatg agccgcagga aattgccggc cagtatcaga ctttcttcgg tgtgcgaaag
     6661 ctcgagcgca cgcaccttcg cgagctccac cgcgggatgc agccagggtc cgtccgtgcc
     6721 gaagagaatc ttgcgcgcgc cggcgcgttg cgcggcttcg gccagcaagt cgaagcgctg
     6781 cacgcccgac gtgtcggtgt gcatgttgcg gtgacgcacg agctgatcga tgagcgccag
     6841 gtgcgcgcgc cagtcgccgg cgaaactcgc aaggtgcgga atgatgaacg gcacgtcggg
     6901 gtattcacgc gccagcagtt cgcaggccga cacttcgccc atgacgtcgt agagcaccgg
     6961 caacccgaat gcgcgcgccg tgtcgcagat ttcacgcgtg atgcggcctt cgtgccgatg
     7021 caccttgatg cccacgaagc cgtactggcg tacggcgacc tccaccagcc ggcggacgcg
     7081 gcccgcatcg cgcaccgcgt tgacgaacgc gaacccgtag aactggcccg gccggctccg
     7141 gacgatacgc gccacctcgc ggttggcgct ttcgtagtcg gaggtgaacg cggcgaacag
     7201 taccgtgcgc cggatgccgg cttgcgacgc gcgctgtaga taggctttca acggcgcacg
     7261 cgtgtcccag ggaccggtga gcccgtcgcc cttccccgcg tggcagtggc agtcgatgat
     7321 catgggtcac gggtttgcgg cgacgatgat cttgtcgatc tcgcgcatca ggtcgagcac
     7381 cttgcctttg agcggatccg cgaggctctt cgacttgaac atatacgcga ccgcgtagcg
     7441 caccttgtcg tgttcgatga tggccgcgtc gtggaacaac gtgttcgtca gtccgcactt
     7501 gttggcgcgc atgcctggca ccacgcgctg gatgaacccg cacgcgcgct tgagcagggc
     7561 ttcgatgcgt gccgacatgc ccttgttcac cagccgcgcc tgggccagca gtgtcatgta
     7621 ggtcacatag gaaagcgcgg agccgcgcac gctcctgtga ccgagaggat ccttggtcca
     7681 gatgatgcgg ctctgattct ggccacaacc ggactcggtt cctccggcca cgtggcaggc
     7741 gccactgata ggtgccggcg cgccacccgt ctggcaatac gtgctgccat accagacacc
     7801 cttgcgcgac ggatgccgca ggcccgactg ccacgcaagc gatgccaggt attcgaaact
     7861 caagcgcagg atgagtcgcg acgcattggg cgtgctgctc ttctcgtcga cgatattcgc
     7921 cattgtggtg ttgagcgcat cactcatctc caccttgagc ggcccggcgc tctcggtgaa
     7981 cttgaacagc cagtcgaagt gcggcttgca ggtgaacttc gcccactccg cgttcgcctt
     8041 cttgcgaagg tccgcggtct tcttgatgcc gccggccttc accatctctt ccagatcgaa
     8101 cagcatctgg tgcgcggcgt agaacagcag gatcttggcg gtgctggcgc ccgactcgtt
     8161 gag
//
LOCUS       DATDEU010000209         6829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1059102, whole genome shotgun sequence.
ACCESSION   DATDEU010000209 DATDEU010000000
VERSION     DATDEU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059102"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /locus_tag="VIV63_05140"
     CDS             complement(<1..1740)
                     /locus_tag="VIV63_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124014.1"
                     /translation="MNPDFRKQLAQAFGLGSETEAEALIAKLRAGDAVDAQVLRKACL
                     ALIDNLPETRAAHVDARLKVALEASNLGLWDWDLTTGNIFVDRTYFAFLGLERDAQVL
                     PITELMGLVSPEDLDAFRSAIADALRGAAPLLQVQHRMRHADGRWVWIETSGTVSSRD
                     SSGRVTRMTGTNANVTERKQLERALSNTLRVLRALLETLPLPVILRDAERRITLVNDA
                     FEKMTGIPRQEIIGKLLDEYSGRIPVPNHRETDDRLFNERKSLRYETSIKGADGTVFD
                     VIVAKTPLMAEDGTITGVASVLTDISEQKRTAEIQEKARVAAEAAVLAKSRFLANMSH
                     ELRTPLNGVVGMASLLENTALDAKQRRFVRTLKSSAEALITLINDVLDLSKAEAGKLT
                     LASGPFELRRELEQVVGLFGARAHDKGIEIAAHIARGVPATVHGDPIRLRQVLGNLVN
                     NAVKFTDAGAVLLAVTMVAPDKGPAMVEFSVTDTGVGIAPAEQKRIFDAFEQADGSVT
                     RKFGGTGLGLAISRQLVELMRGAMDLTSEPGRGSRFSFRIPMGVNALELPPVVPAADL
                     GAIVIGLHPVIRSA"
     gene            complement(1853..2698)
                     /locus_tag="VIV63_05145"
     CDS             complement(1853..2698)
                     /locus_tag="VIV63_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124015.1"
                     /translation="MKKTLALLAAMTFAATAQAQTTAHKLTKLWESEATLKVPEAVRL
                     DAKRKVLYVSNIDGQPWEVDGKGSIAKVGLDGKVIDAAWITGLDCPKGLVLSANGKLL
                     YVADAGGIVVIDIKAGKIKSRIAIPEGIQLNDLAGDDHDTLYVSDSKGKKIYVVKDGK
                     AQVFLDETVLKGPNGLLVHGGTLYVLDNASLNQVQPDKSLKVLADGMPGGVDGLENVK
                     GNDFIVSVWSGAVWYVNGDGSKELLFDGKAVQTSTADIGWDPKTSTVYVPTFFKNSII
                     AFKVD"
     gene            2754..3371
                     /locus_tag="VIV63_05150"
     CDS             2754..3371
                     /locus_tag="VIV63_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEY6124016.1"
                     /translation="MPVSTLFATRVYTKSLTRRGSDPLNARLLRECRQLSVEDAAGIR
                     WSAANYPGGYTSYSSLSRMHQMSPTFAALEKRIDTHVRTFARTLDFDLVGRKLSMTDC
                     WVNVMTRYVVHGLHLHPLASLSGTYYVRTPRGCSGLKFEDPRLDRFMASPPRKARARR
                     ENQVWVTVPAEQGNLVMFESWLRHEVAPNPTEAERISISFNYSWF"
     gene            complement(3435..4043)
                     /locus_tag="VIV63_05155"
     CDS             complement(3435..4043)
                     /locus_tag="VIV63_05155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY6124017.1"
                     /translation="MNFSFSATPASWLILGAFVVISVIALRVAPGIIERNLLRPYWLL
                     RKRQYDTIITSGFIHGDFAHLFFNAFTMFSFGPALERHIGTPKFVALYFVGLLLSSLG
                     TVYKQRNNPDYAALGASGAILSVVFAYVVYYPKNILFLFFVPMPAIVFAVVYLGYTIW
                     ASQNRRDNIAHDAHLDGALTGLIFVGLTDFAAWKRFFGIFLG"
     gene            complement(4048..4911)
                     /locus_tag="VIV63_05160"
     CDS             complement(4048..4911)
                     /locus_tag="VIV63_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124018.1"
                     /translation="MPTTLRAILVFSSLVFSAAAHAGQTTPTVIPLWEEGAPGFESRR
                     AEPEQHKDWWYKNIHNPSLTVFLPPAGKANGTAIVVAAGGGHRELVFNPEGVEPAQYL
                     ASLGVTAFALKYRLFREPGSKYTIDNTAEDIRRAMRTVRSRAAEWNIDPRRIGVMGWS
                     AGGEVAALVAYPPADGKPGAADPVERVSARPDFQILIYPGPLGIPAKVPANAPPLFLL
                     GAADDEYVANVLFDLTRKYHDAGAPIETHIYAQGKHGFNMGQRSQLVSIQRWPQRLAE
                     WLQDRGYTKAN"
     gene            complement(4938..5390)
                     /locus_tag="VIV63_05165"
     CDS             complement(4938..5390)
                     /locus_tag="VIV63_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124019.1"
                     /translation="MMLVLVGGFVAFRLFSKEEAPPRDFTALEFAGADASHTGSCYSL
                     DTVLVANRSGSDATRTWSSPRDDVWTLKLDEIYQGNGGPVRVFQIYTFEKDGDQARLT
                     SVEASEGQSTDVNRNIDLLIKDSKALRATPVDRCLEPGATGYLFKPRR"
     gene            complement(5414..5932)
                     /locus_tag="VIV63_05170"
     CDS             complement(5414..5932)
                     /locus_tag="VIV63_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124020.1"
                     /translation="MLALGPLTACQRSLDEQEVREFIDKADNAARKRFAPEICELRGE
                     TFELHLTFQGHEENFEPTDVTMDRKLFCKEAGKFSRLRQYKLERNSTAIDVANDGRTA
                     RVTSQYVETLPYYEPGMTPTTPDDFREFQVVETTDESVVGIEGGNLVFLRTDASSSQS
                     LVGKSTVDLPYD"
     gene            complement(6574..>6829)
                     /locus_tag="VIV63_05175"
     CDS             complement(6574..>6829)
                     /locus_tag="VIV63_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124021.1"
                     /translation="LIVLRAEPEFAELSVANPGPTLHPETASRLFESLWQSRVESDKR
                     PHFGLGLYIVRLIAEFHGGTARAENLPDNTGAVFTVRLTR"
BASE COUNT         1251 a         2056 c         2182 g         1340 t
ORIGIN      
        1 ggcgctacga atgaccggat gcaggccgat gacgatggcg cccaggtcgg cggccggcac
       61 gaccggcgga agttcgagtg cgttgacacc catgggaatg cggaacgaga agcggctgcc
      121 gcggccgggc tcgctcgtga gatccatggc gccgcgcatg agctcgacca actgccggct
      181 gatggcgagc cccagccccg tgccaccgaa cttgcgcgtg acgctgccgt cggcctgttc
      241 gaatgcgtcg aagattcgct tctgctcggc tggcgcgatg ccaactccgg tgtcggtgac
      301 cgagaattcg accatcgccg gccccttgtc gggcgccacc atcgtcacgg ccagcaacac
      361 ggcgcccgca tccgtgaact tgacggcgtt gttcaccaga ttgccgagca cctggcgcag
      421 gcggatggga tctccgtgca cggtcgccgg cacgccgcgc gcgatgtgcg cggcaatctc
      481 gatgcccttg tcgtgggcgc gcgcgccgaa caggccgacg acctgctcga gctcgcgccg
      541 cagctcgaaa ggcccgctgg cgagcgtcag ctttccggct tccgccttgg acagatcgag
      601 cacgtcgttg atgagcgtga tgagcgcctc cgccgaggac ttgagcgtgc gcacgaagcg
      661 tcgctgcttg gcgtcgagag ccgtgttctc gagtagtgag gccatgccca ccacgccatt
      721 gagcggcgtg cgcagctcat gactcatgtt ggcgaggaag cgcgatttcg ccagcaccgc
      781 ggcttccgcg gccacgcggg ccttttcctg gatttcggcc gtgcgcttct gctccgagat
      841 gtccgtgagc acggaggcga caccggtgat ggtgccatcc tcggccatca atggagtctt
      901 ggcgacgatc acatcgaaga cggttccgtc cgctcccttg atcgaggttt catagcggag
      961 cgactttcgt tcgttgaaca accgatcgtc ggtctcgcgg tgattcggaa ccgggattcg
     1021 ccccgaatac tcatcgagca gtttgccgat gatttcctgg cgcgggatac ccgtcatctt
     1081 ctcgaacgcg tcgttgacca gcgtgatacg gcgttcggcg tcgcgcagga tcacgggcag
     1141 tggcagggtt tccagcagcg cgcgcaagac acgcagcgtg ttcgacaacg cgcgctcgag
     1201 ttgcttgcgc tccgtgacgt tggcattcgt gcccgtcatg cgcgtgacac gtccgctcga
     1261 atcgcggctg ctcacggtgc cggaggtttc tatccagacc caacggccat ccgcgtgcct
     1321 catgcgatgt tgaacctgga gcagtggcgc cgcgccccgg agtgcgtcgg cgatcgcgct
     1381 acggaacgcg tcgagatctt cgggcgacac gaggcccatg agttcggtga tcggcaacac
     1441 ctgggcgtcg cgttcgagcc cgaggaacgc gaaataggtc cggtcgacga aaatgtttcc
     1501 ggtggtgaga tcccaatccc atagaccgag attggatgcc tccaatgcga ctttgagccg
     1561 ggcatcgacg tgggccgcgc gcgtctcggg aagattgtcg atcagtgcga ggcatgcctt
     1621 gcgcagaacc tgcgcgtcca cggcatcgcc ggcgcgcagt ttcgcgatca gggcttcagc
     1681 ctcggtttcc gaaccgaggc cgaaggcctg cgccaattgc ttcctgaaat cggggttcac
     1741 ctgatacgcg cgcgcatggc gcaacctcgg acgctccact cctgtaaacg gtgtcggcag
     1801 agcggccgat tactgagccc cggcggtcag tggtttgtgc gatgggtcac aatcagtcaa
     1861 ctttgaaggc gatgatcgag ttcttgaaaa aagtcggcac gtaaaccgtg ctggttttcg
     1921 ggtcccagcc gatgtcggcg gtgctggtct gcacggcctt gccatcgaac agcaattcct
     1981 tcgatccgtc gccgttcacg taccagaccg cgccgctcca cacggacacg atgaagtcgt
     2041 tgcccttcac gttttcgaga ccgtcgacgc cgccgggcat gccatcggcg agcaccttga
     2101 gcgacttgtc gggctgcacc tgattgaggc tggcgttgtc gagcacgtac aaagtgccgc
     2161 cgtgaactaa caacccgttc ggacccttga gcacggtctc gtcgaggaac acctgcgcct
     2221 tgccgtcctt cacgacgtag atcttcttgc ccttcgagtc ggacacgtac agcgtgtcgt
     2281 gatcgtcgcc ggcgagatcg ttgagctgta tgccttcggg aatggcgatc ctgctcttga
     2341 tcttgccggc cttgatgtcg atgacgacga tgccaccggc atccgccacg taaagcagct
     2401 tgccgttggc ggagagcacg aggcccttcg ggcaatcgag gccggtgatc cacgcggcgt
     2461 cgatgacctt gccatcgagc ccgaccttgg cgatggaacc cttgccgtcg acttcccagg
     2521 gctggccgtc gatgttcgag acgtacaaca ccttgcgctt cgcgtcgagg cgcacggctt
     2581 cgggcacctt gagagtggct tccgattccc agagcttcgt gagcttgtgt gccgtagttt
     2641 gggcctgggc agtggcggcg aaagtcatcg cggccagcag tgcgagagtc ttcttcatgg
     2701 gtacgtcctt gatggaaggg gcggaaagta tagtggcgcg ttatgctcgc cgcatgcccg
     2761 tgagtacctt gtttgccacc cgtgtctaca ccaaaagtct aacgcgccga ggttcggacc
     2821 cgttgaatgc gcggttgctg cgtgagtgcc gccagctcag cgtcgaggac gctgccggca
     2881 tccgctggtc ggcggctaac tatcctggtg gatacacgtc gtacagctcg ctgtcgcgca
     2941 tgcaccagat gtcgccgaca ttcgcagcgc tcgagaagcg catcgacacc catgtccgca
     3001 ccttcgcgcg cacgctcgac ttcgatctcg tgggccgcaa actttcgatg acggattgct
     3061 gggtcaacgt gatgacccgc tacgtggtgc acggcctgca cctgcatccg ctggcgagcc
     3121 tgagcggcac gtactacgtg cgcacgccac gcggctgctc cgggctcaag ttcgaagacc
     3181 cgcgtctcga tcgcttcatg gcgtcaccgc cgcgcaaggc acgcgcacgc cgcgagaatc
     3241 aggtgtgggt tacggtgccc gcggaacagg ggaacctggt gatgttcgag agctggctgc
     3301 ggcacgaagt cgcgccgaat cccaccgagg cggagcgcat ctccatctcg ttcaactact
     3361 cctggttcta gccgattaaa aggggacaga tctattttcc gcggcggaaa atagatctgt
     3421 ccccttttaa tcaactagcc gagaaatatc ccgaagaagc gcttccacgc cgcgaagtcc
     3481 gtcagcccca cgaagatgag tccggtcagc gcgccgtcca gatgcgcgtc gtgcgcgatg
     3541 ttgtcgcgcc ggttctgcga cgcccagatg gtgtagccga gatagaccac ggcaaacacg
     3601 atggccggca tcggcacgaa gaacaggaag agaatgttct tcgggtaata cacgacgtag
     3661 gcgaacacca ccgagagaat ggcgcccgaa gcgccgagcg ccgcgtagtc agggttgttg
     3721 cgctgcttgt agacggtgcc gaggctgctc agcagcaagc cgacgaaata tagcgcgacg
     3781 aacttcggcg tgccgatgtg gcgttcgagt gccggcccga acgagaacat cgtgaacgcg
     3841 ttgaagaaca gatgcgcgaa atcaccgtgg atgaagccgc tggtgatgat ggtgtcgtac
     3901 tgccgcttcc ggagcagcca gtaaggacgc agcagattgc gttcgatgat tccgggcgcg
     3961 acgcgcaacg cgatcacgct gatcaccacg aatgcgccga ggatcagcca cgacgcgggc
     4021 gttgcggaga aactgaagtt catgagctca attcgccttg gtgtatccgc ggtcctgtag
     4081 ccactccgcc agccgttgcg gccagcgctg gatgctcacc agttgcgagc gctgccccat
     4141 gttgaacccg tgtttgccct gcgcgtagat atgcgtctcg atgggcgcgc cggcatcgtg
     4201 gtatttgcgc gtgagatcga acagcacgtt cgccacgtat tcatcgtcgg cggcgcctaa
     4261 caggaacagc ggcggcgcgt tggccggcac cttcgcggga atacccagcg ggcccgggta
     4321 gatgaggatc tggaagtcgg ggcgcgcgct cacgcgttcg acaggatcgg cagcgcccgg
     4381 tttgccgtct gcgggtggat aggccacgag cgccgcgact tcaccgccgg ccgaccagcc
     4441 catgacgccg atgcggcgcg gatcgatgtt ccactccgcg gcgcgcgagc gcacggtgcg
     4501 catggcgcgg cggatgtctt cggcggtgtt gtcgatggtg tatttcgagc cgggctcgcg
     4561 gaacaggcgg tatttcagcg cgaatgcggt gacgccaagg ctcgcgagat attgcgcggg
     4621 ctcgacacct tccggattga acacgagttc gcgatgtccc ccgccggccg ccaccacgat
     4681 ggccgtgccg ttggccttgc ccgcgggtgg caggaacacg gtgagcgatg gattgtggat
     4741 gttcttgtac caccagtcct tgtgttgctc cggttcggcg cggcgggatt caaaacccgg
     4801 cgcgccttct tcccacaggg gaatcacggt cggtgtggtc tgcccggcat gggcggcggc
     4861 gctgaaaacg agtgaggaga aaacgagaat cgcgcggagt gtcgtgggca tggacgaacc
     4921 ttgccacaac tgttgcgtca ccgtctcggc ttgaagagat agccggtcgc gccaggctcg
     4981 aggcatcgat cgacgggcgt cgctcgcagt gccttcgaat ccttgatcag caggtcgatg
     5041 tttctgttca cgtcggtcga ttgaccttcc gacgcttcaa ccgacgtgag gcgtgcctga
     5101 tcgccatcct tctcgaaggt gtaaatctgg aagacccgca ccggcccacc gttgccctgg
     5161 tagatctcgt cgagcttcag ggtccagacg tcatcgcgcg gcgacgacca ggttcgagtt
     5221 gcgtcgctgc ccgagcgatt ggcgaccagc acggtatcga gcgagtaaca cgagccggtg
     5281 tggctggcgt cggcgcccgc gaattcgagc gccgtgaaat cgcgcggtgg cgcttcttct
     5341 ttcgagaaga gccggaaagc gacgaagccg ccgaccagca ccaacatcat tgcgggaccg
     5401 gcgagtggac gcatcagtcg tacggcaggt cgacagtgct cttgccgacg agcgactggc
     5461 tggagctggc gtcggtacgc aggaaaacga gattgccgcc ttcgataccg acgacggatt
     5521 cgtcggtggt ttcgacgacc tggaactcgc ggaaatcatc cggcgtggtg ggcgtcatgc
     5581 ccggttcgta atacggcagt gtttcgacgt actgcgacgt gacgcgcgcg gtcctgccgt
     5641 cgttcgccac gtcgatggcg gtggagtttc gttccagctt gtactgccgg agccgcgaga
     5701 actttcccgc ttccttgcag aagagcttgc gatccatggt cacgtcggtg ggttcgaaat
     5761 tctcttcgtg gccctggaac gtcaggtgca gctcgaaagt ctcgccgcgc agctcgcaga
     5821 tctccggcgc gaaacgcttg cgtgcggcgt tgtcggcctt gtcgatgaat tcgcgcactt
     5881 cctgttcgtc gagacttcgc tggcacgcgg tgagcgggcc cagggctaac aagattgcga
     5941 gtagtgaggt gatgcgggaa atggggacat tcctcgattt gctgtaacaa tcgcgaacac
     6001 tatgcgacgg caccaggccg cgcaggtttg ttgcatgaaa atgaggaatg tccccatttc
     6061 cagccaaaaa aaagccccgc agagcggggc ttgttagtag gtcgtcgtcg acttaggtaa
     6121 tcgctgtcag tcgatcgcgt cgactacacg gactgctata ttttcggctg ggacgttggt
     6181 gacctttcgg cctccggcta aacaattaag cccgcgtgtc agtccacgct ttgatcgtcc
     6241 tcgccagtag ccaaaactaa gctaccgact gaacccgtgc ccgtgagcaa atcctgaagt
     6301 ttttctgtat cactaaaccg ttgtgttctc cgcgcatcag aaagtgttgc agtgcagaac
     6361 aaacaaacct tcgtaatcct ttcaaccgga ctaacaacct tgtattcagg tacttagctg
     6421 tggtttcgag actacgcgag acgggatcat acgccgaatc tttcttgcca caatgtctca
     6481 atggcgcgac atcgagcaat cttgtgaact gtatcgacag aatttacggg ggctggaggt
     6541 gaccggatgc gcgaaatcgg aggcgggatc gcctcaacgg gtcaggcgca cggtgaaaac
     6601 cgccccggtg ttgtcgggca ggttctcggc ccgggcagtg ccgccatgga attcggcgat
     6661 gagcctcacg atgtagaggc cgagcccgaa gtggggccgc ttgtcgcttt cgacgcgtga
     6721 ctgccacaac gactcgaaca gccggctggc ggtttccgga tgcagcgtgg gcccaggatt
     6781 ggccaccgag agctcggcga actccggctc cgcgcgcaga acgatcaat
//
LOCUS       DATDEU010000210         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_621159, whole genome shotgun sequence.
ACCESSION   DATDEU010000210 DATDEU010000000
VERSION     DATDEU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621159"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VIV63_05180"
     CDS             complement(<1..1102)
                     /locus_tag="VIV63_05180"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEY6124022.1"
                     /translation="MQSHAIALLDTLSIGIMLGALLVLAGIMSSLVATRFGAPLLLVF
                     LIVGMLAGEAGPGGIKFDDVRVAYVVGSIALALILFDGGLRTRLTTFRSVLAPAAALA
                     TVGVMITAGLTAPVARYLLDMSWSESLLVGAVVASTDAAAVFFLVHARGLRLRPRVGA
                     TLEVESGSNDPFAVLLTVLLVEYLSAGEGSWGHVLAVLAEQAVLGTIIGILGGRAIVF
                     VLNRLALAQGLHAPFVTTSALVIFGLASGLHASGFLAVYLAGLVVGNRPTRAHNTVVV
                     FLDAVTWLVQIVMFVMLGLLVWPQRLLESIWPALAIAATLMFIARPVAVFVCLAPFRY
                     HWREKAFISWVGLRGAVGIFLASIPLLVDLPYA"
     gene            complement(1241..>2363)
                     /locus_tag="VIV63_05185"
     CDS             complement(1241..>2363)
                     /locus_tag="VIV63_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6124023.1"
                     /translation="LFALALPIAITLGKVAAFVAIMLIVGKRVIPWILHYVAHTGSRE
                     LFRLSVLAIALGVAFGAAMLFDVSFALGAFFAGMMLSESELSQRAASETLPLRDAFAV
                     IFFVSVGMLVDPTIVLREPIPLLATVLIIVVGKSLAAFVIVRLFGHPTSTAFTIAASL
                     AQIGEFSFILAGLGIELTLLPDRGRDLILAGAIVSILLNPLLFAALDHWLAKAEAKSA
                     TDAAGPAEIGARASVPHTRLTDHVVLVGHGRVGSFISAAMPPQTALLVIEDDADRVAT
                     LQGEGREAIAGNAADPEVIGAANLAAARCLLVAIPDAFEGGQVVAQARELNGALPIIA
                     RAHSEVEIEHLRKHGATKVVMGEHEIAKAMIADIPPGRA"
BASE COUNT          489 a          790 c          792 g          292 t
ORIGIN      
        1 aggcgtaagg cagatcgacg agcagcggaa tcgacgccag gaaaatgccg accgctccgc
       61 gcaggccgac ccaagatatg aaagccttct cccgccagtg ataacggaac ggcgcgaggc
      121 agacgaacac cgccaccggg cgggcaatga acatgagggt ggccgcgatc gcgagcgcgg
      181 gccagatgct ctcgagcagg cgctgcggcc acaccagcag gccgagcatc acgaacatca
      241 cgatctgcac aagccaggtg acggcatcga gaaacaccac cacggtgttg tgcgcgcggg
      301 tcgggcggtt gccgaccaca aggccggcga gataaacggc aagaaagccc gaggcatgca
      361 gccccgaagc gaggccgaag atgacgagcg cgctcgtggt cacgaacggc gcgtgcaggc
      421 cctgcgccag cgcgagccgg ttgagcacga acacgatggc ccgcccgccg agaatgccga
      481 tgatggtgcc gagcacggct tgctcggcca gcaccgccag cacgtgtccc cacgaccctt
      541 cgcccgccga cagatactcg accagaagga cggtgagcag gaccgcgaac ggatcgttgc
      601 tgccggattc gacctcgagc gtagcgccga cgcgggggcg caggcgcaag ccgcgggcat
      661 ggaccaggaa gaacaccgcc gcggcatcgg tcgaggccac gaccgcaccc accagcaacg
      721 actcgctcca gctcatgtcg agcaggtatc tggcgaccgg ggcggtcaac cccgccgtga
      781 tcatgacgcc gacggtcgcc agcgcggccg cgggcgccag cacgctgcgg aaggtcgtaa
      841 gccgcgtacg caagccgccg tcgaacagga tcagcgccag cgcaatcgag ccgaccacat
      901 aggccacccg cacgtcgtcg aacttgatgc cgcccggtcc ggcctcgccc gcgagcatgc
      961 cgacgatcag gaacaccagc agcagcggcg ccccgaaccg ggtcgcaacc aggctcgaca
     1021 tgatgccggc caggaccaga agcgcaccga gcatgatgcc gatgctgagc gtgtcgagga
     1081 gggctatcgc gtgcgactgc atgattccca ttggaaaggc tgcaacgcgc agcggcaatg
     1141 ataaacattc ggcaggtcgc aaaatttccg ccggcgcgtg gcgccggact gggacgacgt
     1201 ccgaaccggg ctgggattgc gcgaagctgg ccgacccgcg tcaggcgcgt ccgggcggaa
     1261 tgtcggcgat catcgccttg gcgatctcat gctcgcccat gaccaccttg gtggcgccgt
     1321 gcttgcgcag gtgctcgatc tcgacctcgg aatgggcccg ggcgatgatc gggagcgcgc
     1381 cgttgagctc gcgagcctgc gccacgacct ggccgccctc gaacgcatcg gggatcgcga
     1441 ccagcaggca ccgggcggcg gccaaattcg cggcgccgat cacttcggga tcggcggcat
     1501 tgcccgcgat cgcctccctc ccctcaccct gaagcgtggc gacccggtcg gcgtcatcct
     1561 cgatcaccag caatgcggtc tgcggcggca tcgccgcgct gatgaagctg ccgacgcggc
     1621 cgtggcccac cagcaccacg tgatcggtga gccgggtgtg tggaacagat gcgcgggcac
     1681 ctatttccgc tgggccggct gcgtccgtgg cggatttcgc ctcggccttc gccaaccaat
     1741 ggtcgagcgc ggcaaacaac aacggattga gcaggatcga aacaatagcc ccggcgagga
     1801 tcagatcgcg ccctcggtcc ggcaacagcg tcaactcgat gccaagcccg gcgaggatga
     1861 acgaaaactc cccgatctgc gccaggctcg ctgcgatggt gaacgcggtg gaggtgggat
     1921 gaccgaacag ccgcacgatc acgaacgccg ccagcgattt tcccaccacg atgatcagca
     1981 cggtcgccag cagggggatt ggttcccgca gcacgatggt ggggtcgacc agcatgccga
     2041 ccgaaacgaa gaagatcacc gcgaaggcgt cgcgcagggg tagcgtctcg gaggctgcac
     2101 gctggctcag ctcggattcg ctcagcatca tgccggcgaa gaatgcgccg agcgcgaacg
     2161 acacgtcgaa cagcatcgcc gcgccgaagg cgacgccgag cgcgatggcc agcacggaca
     2221 gacgaaacag ctcgcgcgag ccggtatgcg ccacgtaatg caggatccag gggatcacgc
     2281 gcttgcccac gatcagcatg atggcgacga acgccgcgac cttgcccagc gtgatcgcga
     2341 tcggaagggc gagcgcgaac aag
//
LOCUS       DATDEU010000211         6581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1059479, whole genome shotgun sequence.
ACCESSION   DATDEU010000211 DATDEU010000000
VERSION     DATDEU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059479"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1509)
                     /locus_tag="VIV63_05190"
     CDS             complement(85..1509)
                     /locus_tag="VIV63_05190"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6124024.1"
                     /translation="MFHRRFTGRISDWRRAFESESLEQRRAAAEDLMRACFEEKNPTD
                     AVPLIESLLRDPDDEIGECATSALCRCGTAALPVVERMSASASAHLRERACYIIGHLS
                     CETQPLHAVVFAALSDLEPGVRAQAAFALSRMADRSRATVEALAAAVHDPAPKVRLWA
                     LEAFRVFSLDEEVPASLASHGATILSALADEDPDVRKSALSTLEEFEMPATQILTALL
                     DRLRVESNKEVLMNIRLVIFSRELQEDLAQHLPALLTAADANPSASALVFEICGDLGV
                     RATAALPQLETVAASPGTSYEAVEALWKITGSSAKCVPALERLLERDWLDASRALELL
                     SKMTDSRRYVIPMLRHALPDSPDEAAGMICDLGTAAAPLLPELARVIEEHWEEPDWDL
                     MWALVDALYAIESSETLAVSTLGKLLTHPSPRVRVIAVKGLGRAGPAARAALGTLEKA
                     VQDEDREVAKAARAAIREVGRPAN"
     gene            1610..2005
                     /locus_tag="VIV63_05195"
     CDS             1610..2005
                     /locus_tag="VIV63_05195"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY6124025.1"
                     /translation="MTNDIYLTDREADVMQVLWDHGPSVVNEVKEKLHDELAYTTVLT
                     ILRTLEQKGYVKHEEEGRVHRYAAAVKENAARKSALQHLTGKLFKGSSELLFTHLVSD
                     QKLSADQIKRMRDVLAESTPKKADSKKEK"
     gene            2006..3598
                     /locus_tag="VIV63_05200"
     CDS             2006..3598
                     /locus_tag="VIV63_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY6124026.1"
                     /translation="MLAWMLYVIMVTLLLSVGAYVAERAARLKRGGTRWIWITAIVAS
                     LLLPTVIASVSVQLPGFMTPEVSQKMVALRDATTQALSPVFWISGSAAEPTGWRDFDA
                     VLKSVWRAASVAMLLALIASGIHLFSRKRRWHQGTVAGAAVYITDEVGPAVVGLVRPR
                     IVVPRWVTMALPSHQAAVIAHEQSHLEARDPQLFTFALALLVFMPWNLPLWWQLRRLR
                     YAIEIDCDARVLKGGVDPAHYGETLIVVGERQSAYVGAVAAMSESKSFLEERIEHMIR
                     KPVRWRRLGAATLATFAIAVTALAAQVSPPNAETPAPVAGKPTPVERKEIKLDNAVLD
                     RYAGSYKLGDTMIFTVKREGKYLQAQLTGQQFIEMYPESDTKFFYKAIDAQLEFKPDS
                     GGGTESATLFQNGQVVTMPRIDAAVAQQIQTDVAAKIAGGAPTPGSEVALRRLSDGIA
                     SGKPDYDVMSPFLADALKQQLPHMQPGIAALGPLKSVTFRNVTGQGHDLYLATYEKGT
                     LAWRISMSPDGKIANALVTPDF"
     gene            complement(3662..4636)
                     /locus_tag="VIV63_05205"
     CDS             complement(3662..4636)
                     /locus_tag="VIV63_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY6124027.1"
                     /translation="MPRAIRIQEVGGPEVMLLKQVDVPAPAAGEVQVEHTAIGLNYID
                     VYDRTGLYAQAMPGGLGREAAGRVSAIGKRVRGFRVGDRVAYVMSPPGTYADLRNVPS
                     TRVVKIPSGVSDEHAAVLALKGMTACFLLRHCFRVERGDVIVVHAAAGGVGSLLSQWG
                     RKLGAVVIGVVGSEEKAALAKRSGCKHVLVRGRDKIAASVRKLTMGLGAHVVYDGVGK
                     DTFIESLDSLRKRGMMVLYGSASGPVPPFSPGELVKRGSLFLTRPTMFDYTESREDLE
                     AIARETFAAAKKGWIKVQVNQRYKLVDAAQAHRDLEARKTTGASVIIP"
     gene            complement(4661..5974)
                     /locus_tag="VIV63_05210"
     CDS             complement(4661..5974)
                     /locus_tag="VIV63_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY6124028.1"
                     /translation="MRALSIRVPAALGLLLCPILSFAQEATPGVPAADTGDTAWIIAA
                     TALVLFMTLPGLALFYAGLVRSKNVLSILMQCFAITGVASLVWLGVGYSLTFSDGGAA
                     QGFIGGLDKAFLMGVTRDAVSGTIPETLFFMFQMTFAIITPALVIGGFAERMRFSAVL
                     GFTVLWLLFVYVPLAHWVWGGGWLAKLGVMDFAGGIVVHVSAGVAALVSAVVLGRRRG
                     FPHTAMTPHSLPLTVAGAGMLWVGWFGFNAGSALAANGSAGMAMLTTHMGASAGALAW
                     MCVEWKKYGKPSMLGVVTGMVAGLGTITPASGFVGPLGAVCIGLIAGTVCFFATQLLK
                     RKLEIDDSLDVSPVHGVGGVIGSLLTGVFAAVWLGGTGFTVQTSVAAQLGVQALGVVA
                     AAAWCALLTWLILKLLDAIFGLRVGEEKETEGLDLAEHGERGYSP"
     gene            complement(6149..6487)
                     /gene="glnK"
                     /locus_tag="VIV63_05215"
     CDS             complement(6149..6487)
                     /gene="glnK"
                     /locus_tag="VIV63_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY6124029.1"
                     /translation="MKLVTAIIKPFKLDDVRAALSEIGVSGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFVPKTKIEVAVRSDLVDQVVEAILKAAKTGKVGDGKIFITDIDRVIRI
                     RTGETDNSAL"
BASE COUNT         1290 a         2131 c         2016 g         1144 t
ORIGIN      
        1 accgacatcg tcgaagtcgt gcgctcgggc gcgctggcgg tcggccgcaa tgcgcgggcg
       61 ctgcacgtcg tacgctgatc gcggctagtt agccggcctc ccgacctcgc gaatggccgc
      121 gcgcgcggcc ttcgcgactt cccggtcttc gtcctgaacc gctttttcga gggtgccgag
      181 cgcggcacgc gcggcgggac ctgcccggcc gagtcccttg actgcgatga cgcggactcg
      241 cggactaggg tgcgtcaaca gtttcccgag agtcgacacg gccagggttt cgctgctctc
      301 gatcgcgtac agcgcgtcga cgagtgccca catgagatcc cagtcgggtt cttcccaatg
      361 ttcttcgatg acgcgcgcaa gctccggcaa gagcggcgcg gcggccgtcc cgagatcgca
      421 gatcatcccg gcggcttcat cgggtgagtc cggaagcgcg tggcgcaaca tgggaatcac
      481 atagcggcgg ctgtccgtca ttttcgacag cagttcgagc gcgcgcgatg cgtccagcca
      541 atcgcgctcc agcagtcgct cgagcgcggg aacgcatttc gccgagcttc cggtgatctt
      601 ccagagggct tcgacagcct cgtatgacgt acccggactc gctgcgacgg tttcgagctg
      661 cggaagcgcc gcagtggctc gtacacccag gtccccgcag atttcgaata ccaacgccga
      721 ggccgacggg tttgcatcgg ccgcggtaag cagcgccggc agatgctgcg ccaggtcttc
      781 ctgcagttcg cgactgaaga tgacgagccg aatattcatc agcacttctt tattcgattc
      841 gacccgcagc ctgtcgagca gagccgtcag aatctgcgtt gcgggcatct cgaactcttc
      901 gagcgtggac agcgccgact tgcgcacgtc ggggtcctca tccgcgagcg cggaaagtat
      961 cgttgcgcca tgggacgcaa gcgaggctgg tacctcttca tcaagactga atacgcgaaa
     1021 cgcttccagt gcccacaagc gaactttggg cgccgggtcg tgcacggctg ccgccaacgc
     1081 ttcgacggtc gcccgcgatc gatccgccat gcggctcagc gcgaacgccg cttgcgctcg
     1141 cacgcccggc tcgaggtcag aaagcgcagc gaacaccacg gcatgcaacg gctgcgtttc
     1201 gcacgagaga tgaccgatga tgtaacaggc acgttcgcgc agatgggctg aggctgacgc
     1261 agacatgcgc tcgaccactg gcaacgccgc cgtgccgcat cggcacagtg cggaagtcgc
     1321 gcactcacct atctcatcgt ccggatcccg cagcagcgac tcgatcaagg gaaccgcgtc
     1381 cgtcggattc ttttcctcga agcatgcccg catgaggtct tcggccgcgg cacggcgctg
     1441 ctcgagcgat tccgactcga acgcgcggcg ccaatcggaa attctgccgg taaatctgcg
     1501 gtgaaacatg cgccggacca taacacgcgg tctcactact agacttctag ttgacaactc
     1561 gggggcggtt ggcgtttact actagacatc tagtagtgag gcgcctggca tgaccaacga
     1621 catctatctc accgaccgcg aagccgacgt catgcaggtg ctgtgggatc acggcccttc
     1681 ggtggtcaac gaggtgaaag agaagctgca cgatgagctc gcctacacca cggtgctcac
     1741 gattctgcgc acgctcgagc agaagggtta cgtgaagcat gaagaggaag gccgtgtgca
     1801 tcgttatgcc gctgcggtga aggagaacgc cgcacgcaag agcgcgctgc agcacctcac
     1861 cggaaagttg ttcaagggct catccgaact cctgttcacg cacctggtgt cggaccagaa
     1921 attgtccgcg gaccagatca agcgcatgcg cgacgtgctc gcggaaagca cccccaagaa
     1981 agccgactcc aagaaggaga agtaaatgct tgcctggatg ctgtacgtca tcatggtcac
     2041 gttgctgttg tcggtcggcg cgtacgttgc cgagcgcgca gcgcgcctca agcgcggcgg
     2101 cacgcgctgg atctggataa ccgcgatcgt cgcctcacta ctgctgccca ctgtcatcgc
     2161 gtcggtgtcc gtgcagttgc caggtttcat gacccctgag gtctcgcaga agatggtggc
     2221 cttgcgtgac gccaccacgc aggcgttgtc ccctgtcttc tggatctcgg gcagcgccgc
     2281 ggagcctaca ggctggcgcg atttcgatgc cgtactgaaa agcgtttggc gtgcagcatc
     2341 tgtcgcgatg ctgctggcgc tgatcgcgag cggcatccac ctgttctctc gcaaacgccg
     2401 ctggcaccag ggcacggtcg ccggcgcggc ggtgtacatc accgatgaag tgggtcccgc
     2461 ggtagttggg ctcgtccggc cgcgaatcgt cgtgccgcgc tgggtcacga tggccctccc
     2521 gtcgcaccag gcggcggtca tcgcccacga gcaatcccat ctcgaagcgc gagatcccca
     2581 gctattcacg ttcgcactcg cgctgctggt tttcatgcca tggaacctgc cgctctggtg
     2641 gcagctgcga cgtctgcgct acgccatcga aatcgattgc gacgcacggg ttctcaaggg
     2701 cggggtagac cctgcgcact acggcgagac gctgatcgtc gtgggtgaac gtcaatcggc
     2761 gtacgtcggc gcggttgcgg cgatgtcgga atccaaatca tttctcgaag agaggatcga
     2821 acacatgatc agaaaacccg tgaggtggcg tcgactcggg gcggccacgc tcgcaacttt
     2881 tgccatcgcc gtcaccgcgc tcgccgcaca ggtcagccca cccaatgccg agacgccagc
     2941 gccggtggcc ggaaagccga cgccggtcga acgcaaggag atcaagctcg acaacgcggt
     3001 gctcgatcga tatgccggga gctacaagct cggcgacacc atgattttca ccgtgaagcg
     3061 cgaagggaaa tacctgcagg cgcagcttac cggccagcaa ttcatcgaga tgtatcccga
     3121 gagcgatacg aagttcttct acaaggcgat agatgcgcag ctggaattca agccggattc
     3181 cggcggtgga accgaatccg ccaccctgtt ccagaacggc caggttgtga ccatgcctcg
     3241 tatcgacgcc gctgtggctc aacagattca gacggatgtc gccgccaaga tcgccggcgg
     3301 tgcaccgaca cccggcagcg aagttgcgct gcgacgcttg agtgacggca tcgccagcgg
     3361 caagcctgac tacgacgtga tgagcccgtt cctggcggac gccctgaagc agcagcttcc
     3421 gcacatgcag ccgggtattg cggcgctagg cccgctgaag tccgtcactt tccggaatgt
     3481 caccgggcag gggcatgatc tgtatctcgc gacgtacgag aagggcactt tggcctggcg
     3541 aatatccatg tcgccggacg gcaagatcgc gaacgcgttg gtgacgccgg acttctgaac
     3601 ggcccactcg agcgctcaca ccaccggcac ctgctgcaac aggggccggt ggtttgcgcg
     3661 tttagggaat gatgacgctc gcgcccgtgg tcttgcgcgc ctcgagatcg cgatgcgcct
     3721 gcgcggcatc gaccagcttg tagcgctgat tgacctgcac cttgatccag cctttcttcg
     3781 ccgccgcgaa cgtctcacgc gcgatcgcct cgagatcttc acgcgactcg gtgtagtcga
     3841 acatcgtcgg ccgcgtcagg aacagcgagc cacgcttcac gagttcaccc ggagagaacg
     3901 gcggcacggg tccggaggcg ctgccgtaca gcaccatcat gccgcgcttg cgcaacgagt
     3961 cgagcgactc gatgaaagtg tccttgccga cgccgtcgta cacaacgtgc gcgccgagcc
     4021 ccatggtgag tttcctgacg ctcgccgcga tcttatcgcg gccgcgcacc agcacgtgtt
     4081 tgcagccgct cctcttcgcc agcgcggctt tctcttcgct gccgacgacg ccgatgacca
     4141 ccgcgcctaa cttcctgccc cactgtgata gcagtgaccc aacgccaccc gcagctgcgt
     4201 gaaccacgat gacgtcaccg cgttcaacgc gaaagcagtg acgcagcaga aaacaggcag
     4261 tcatgccctt gagtgccagc actgcggcat gttcatcgct cacgccagaa gggatcttca
     4321 cgactcgcgt cgagggaacg ttgcgcagat ccgcataggt tccaggcggc gacattacgt
     4381 acgcgacacg gtcgccgacg cgaaatccgc gcacacgctt tccaatcgcg gacacacgcc
     4441 cggcggcttc gcgcccgagc ccgcccggca tcgcttgcgc atacagaccc gtgcgatcgt
     4501 agacgtcgat gtagttgagc ccgatggccg tgtgctcaac ctgcacctcg cctgcagcag
     4561 gcgcgggtac gtcaacttgc ttgaggagca tgacctcggg gccaccgact tcttgaatac
     4621 gaatggcgcg tggcatcggc tgctcctcga atgggcggaa tcaaggagag taaccccgct
     4681 caccgtgctc ggcgagatcc agtccttcgg tttctttctc ttcgccgacg cgcagaccga
     4741 agatggcatc gagcaacttg aggatcagcc aggtcagcaa cgcgcaccac gccgccgccg
     4801 cgaccactcc gagtgcctgc acgcccagct gcgcagcgac gctcgtctgc accgtgaacc
     4861 cggtgcctcc gagccagacg gctgcgaaca cgcccgtcag caacgaacct atgacaccgc
     4921 cgacaccgtg cacgggggag acgtcgagcg agtcgtcaat ctccagcttg cgcttgagga
     4981 gttgcgtggc gaagaagcag accgttcccg caatgagacc gatgcataca gctcccagcg
     5041 ggccgacgaa tccggacgcc ggcgtgatgg tgccaagccc ggccaccatt ccggtaacca
     5101 cgccgagcat cgacggcttg ccgtatttct tccactcgac acacatccac gcgagcgcgc
     5161 cggcggacgc gcccatgtgg gtggtcagca tggccatgcc ggcggagcca ttcgcggcca
     5221 gcgcactgcc agcgttgaac ccgaaccagc ccacccagag catgcccgcg cccgcgacgg
     5281 tgagcggcag gctgtgcggc gtcatcgcgg tatgcggaaa gccgcggcgc ctacccagca
     5341 cgaccgcact gacgagcgcg gccacaccgg cgctgacatg caccacgatg ccgcccgcga
     5401 aatccatgac gccgagcttg gctaaccacc cgccgcccca gacccagtgc gccagcggca
     5461 catagacgaa caacagccag agcacggtga acccgagcac cgcagaaaag cgcatgcgct
     5521 cggcgaagcc gccgatgacg agcgcgggcg tgatgatggc gaaagtcatc tggaacatga
     5581 aaaacagggt ttcggggatc gtgccgctga ccgcgtcgcg cgtgacaccc atcaggaagg
     5641 ctttgtcgag cccgccgatg aaaccctgcg cagcgccgcc atcggagaac gtgagcgagt
     5701 agcccacgcc cagccagacc agcgacgcga caccggtgat ggcgaagcac tgcatgagga
     5761 tggagagcac gttcttggag cgcaccaggc ccgcgtagaa caacgcgaga ccgggaagag
     5821 tcatgaagag gaccagggcc gttgcggcga tgatccaggc ggtgtcgccg gtatccgcgg
     5881 cgggtacgcc aggagttgct tcctgcgcga aagaaagaat cgggcacagc agcaagccga
     5941 gcgccgccgg cacccggata gatagagcac gcatttttag atccccactc gtcagtggct
     6001 cacgccacgt cacgaattca atcgcccgcc cgctgcctga attggcagaa ggtcttgtta
     6061 gtggtcagca atcacatcga aacaacggcc tgccgctgga agggcagcag gccgtcgtac
     6121 atcaactgtt gagagacttc gaaccccgtt acagggcgct gttgtccgtc tcgcccgtac
     6181 ggatacggat gacgcgatcg atgtccgtga tgaagatctt gccgtcgccg actttgccgg
     6241 tcttggctgc cttgaggatg gcttcaacca cttggtcgac gaggtcgcta cgcacggcga
     6301 cttcgatttt ggtcttgggg acgaagtcga cgacgtattc agcgccgcga tagagctcag
     6361 tgtggccgcg ctggcggccg aagcccttaa cctccgtcac cgtcatgccg gaaacgccta
     6421 tctcggacag cgctgcacgt acgtcgtcga gtttgaaagg cttgatgatg gcggtgacca
     6481 atttcatttc tttatctccc aattctttag tgaatctgga cgccgacatc ggcgagcctt
     6541 aatcgcagtg caccttccgt gccagtatcc cgaaagctca a
//
LOCUS       DATDEU010000212         4624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_475985, whole genome shotgun sequence.
ACCESSION   DATDEU010000212 DATDEU010000000
VERSION     DATDEU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475985"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VIV63_05220"
     CDS             <1..551
                     /locus_tag="VIV63_05220"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6124030.1"
                     /translation="FIDAPVSGGVEGAQKGTLAIMTGGDPAAFEAARPILAAMGKTIE
                     HFGPAGSGQATKATNQIMCAGIIRACAEAMAFAGAHQLPLDRVVSTLGAGAGSSWYFV
                     HRAPNMVRGAYPAGFRIRLHAKDLGICRDMAARFGVSLPVVDSMLAEYAQLMDQGYGD
                     EDISATHRLKQALFREAAKPSA"
     gene            548..1621
                     /locus_tag="VIV63_05225"
     CDS             548..1621
                     /locus_tag="VIV63_05225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6124031.1"
                     /translation="MNRPPPPSPSPGEEPRQLYLPNFCSPAAALTIIVICELTALLLA
                     LARNDAALGFWSDLGLTSMFLLWIALCGAALLCLLRGYLARKSVAVSAAIVLTITVGL
                     VIIVSAAAYAYGAGVLFPDFHWSFLVRNAGITFVVTALALRYFYVTHEWRHNVELQAK
                     ARVHALQARIRPHFLFNSMNTIAALTRSNPARAEEAVQDLADLFRANLNEKRNQIALA
                     EEIDVARTYQRMEQLRLGDRLRVDWKIDSLPNEALVPGLTLQPLLENAIYHGIEPLAE
                     GGVVTVTGEFNKGMITIVVRNPLPVANLTVRDGNRLALANIKERLELMYGDRATVKAG
                     RFDDQYIVTLRFPFTETPVTGVT"
     gene            1624..2355
                     /locus_tag="VIV63_05230"
     CDS             1624..2355
                     /locus_tag="VIV63_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY6124032.1"
                     /translation="MRVLIVDDEPPARERLRSMLAEAGDFEVAGEAGNGEQAIDMVDR
                     LTPDVVLLDVRMPGVDGLEVARHLAGLPTPPAIIFTTAFDEYALQAFDSEAVAYLLKP
                     IRAEKLKAALAKAEKLSRAQLQQVAAATSATAHRTHIGARGRDGLKLIPIEEVFCFQA
                     DQKYTTVKHLKGEDLIEDSLKTLEDEFAAYFLRIHRNALVNTKYLERIARDASGQHFV
                     HLRGLPVALEVSRRMAGDLKDRFRI"
     gene            complement(2356..2847)
                     /locus_tag="VIV63_05235"
     CDS             complement(2356..2847)
                     /locus_tag="VIV63_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124033.1"
                     /translation="MRLRTLTVVSGAVLLTACAPYRIDHRHLVLDETPGLVVLERSTT
                     AVDSQGEPLKSGKVGFPTEVVLKRERYDVLIELSPSSVPMLFLSARTAQGTVLNVEGA
                     HVRRVHPDAEVLGYQFGFMVEEADGEPLEFVVRAADGTELGRERLTYKIRSRGVAYGI
                     EWI"
     gene            complement(2858..3451)
                     /locus_tag="VIV63_05240"
     CDS             complement(2858..3451)
                     /locus_tag="VIV63_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6124034.1"
                     /translation="MSGNAQTEFHVHIEPGGHDIRVPSGENVLAAALAAGIPLPYSCR
                     AGRCASCKAKLLAGAIEYPAGIPPGITPAEVERGEVLLCQARPRSDLSIESRRVPVRA
                     SNAAMCEIVALDPLPLGALRVQLRFTEGTLEARPGQFVDVRNHAGDSERMAVIGARPG
                     EIDVECLQDGSVLRAWLDADAVSGSSLCVAGPFDRPR"
     gene            complement(3448..4380)
                     /locus_tag="VIV63_05245"
     CDS             complement(3448..4380)
                     /locus_tag="VIV63_05245"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HEY6124035.1"
                     /translation="MRLVNNLANITAAWLLCAATAVPAFAQDSPEAEPELVADSTEPV
                     AEEAAIPELKFATGDIVLPNKVATLHLGEKYHYLAPEEASQLLQLWGNPPDNSTQGAI
                     YPAGVNPLKQGGWAVFLTYLDEGHVDDSDAKDIDYDDMLKDMKEGTESNNDARKEAGY
                     PAMHLIKWAEPPRYDAATKKLYWAKEISTDDSKTHYLNYDVRVLGREGVLSMEAVATM
                     DQLQQVRTDMRPLLDVAEFNEGYRYAEFDSKTDRVAAYGLGALIAGGVAAKAGLFAKL
                     FALLLAGKKFIIIGVIALGGVLAKMFGKKKDEAA"
     gene            4434..>4624
                     /locus_tag="VIV63_05250"
     CDS             4434..>4624
                     /locus_tag="VIV63_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY6124036.1"
                     /translation="MFENPTKPEIDEFLRRIRTVAILGLSETPGRPSHNVAKALQKFG
                     YRIVPVNPTATEILGERAWP"
BASE COUNT          816 a         1577 c         1425 g          806 t
ORIGIN      
        1 gtttcatcga cgcgcccgtc agcgggggtg tcgaaggcgc gcagaagggc acgctcgcca
       61 tcatgacggg cggcgacccc gccgccttcg aggcggcacg acccatcctc gcggcgatgg
      121 gcaagaccat cgaacacttc ggtccggccg gctcgggcca ggccaccaag gccaccaacc
      181 agatcatgtg cgccggcatc atccgcgcct gcgcggaagc catggcattc gcaggcgcgc
      241 accagttgcc gctcgatcgc gtggtgtcga cgctcggcgc cggcgcgggg tcgagctggt
      301 acttcgtgca tcgcgcgccg aacatggttc gcggcgccta ccccgccggc ttccgaatca
      361 ggctgcatgc caaggatctc ggcatctgcc gtgacatggc ggcgcgtttc ggcgtgtcgc
      421 tgccggtggt cgactccatg ctcgccgaat acgcgcagct catggaccag ggttacggcg
      481 atgaagacat ctccgcgacg caccggctca agcaggcgtt gttcagggaa gccgcgaagc
      541 cttcggcatg aaccgcccgc cgccaccctc accttctccc ggcgaggaac cgcgacagct
      601 ctatctaccc aatttctgct cgccggccgc ggcgctcacc atcatcgtca tctgcgagct
      661 caccgcgctg ctgctggcgc tggcgcgcaa cgacgcggca ctgggtttct ggagcgatct
      721 cggactcacc tccatgttcc tgctgtggat cgcgctgtgc ggcgcggccc tgctctgcct
      781 gctgcgcggt tacctcgcgc gaaagagcgt cgcggtcagc gccgccatcg tgctgaccat
      841 cacggtgggc ctggtcatca tcgtgtccgc ggcggcctat gcgtatggcg ccggcgtgtt
      901 gttcccggac ttccactggt cgttcctggt tcgcaacgcc ggcatcacgt tcgtggtcac
      961 cgcgctcgcg ctccgctatt tttacgtcac gcatgaatgg cggcacaacg tggaactgca
     1021 ggccaaggcg cgcgtgcatg ccttgcaggc gcgcatccgt ccgcacttcc tcttcaacag
     1081 catgaacacc atcgcggcgc tcacccgcag caacccggcg cgcgccgaag aggcggtgca
     1141 ggatctcgcc gatctgttcc gcgccaacct gaacgaaaag cgcaaccaga tcgcgctggc
     1201 cgaagagatc gacgtggcgc ggacgtacca gcgcatggaa caacttcgcc tcggcgatcg
     1261 cctgcgtgtg gactggaaaa tagattcgtt gccgaacgaa gcgctggtgc ccggcctcac
     1321 actgcagccc ctgctcgaaa acgccatcta tcacggcatc gagccgctgg ccgaaggcgg
     1381 cgtggtcacg gtcacgggcg aattcaacaa gggcatgatc accattgtcg tgcgcaaccc
     1441 gttgccggtc gcaaacctca ccgtgcgtga cggcaatcgc ctggcgctcg ccaacatcaa
     1501 ggaaaggctc gagctcatgt atggcgatcg cgccacggtc aaagccggcc gcttcgacga
     1561 tcaatacatc gtgacgctgc gtttcccgtt cacggaaacg ccggtgacgg gcgtcacgtg
     1621 agcctgcgcg tgctcatcgt cgatgacgag cctccggcac gcgagcgcct gcgctccatg
     1681 ctcgcggaag ccggcgactt cgaagtcgcg ggcgaagccg gcaatggtga gcaggccatc
     1741 gacatggtgg accggctgac gcccgatgtg gtcctgctcg acgtgcgcat gcccggggtc
     1801 gatggcctcg aagtggcgcg ccatctcgcc gggcttccca cgccgccggc gatcatcttc
     1861 accacggctt tcgacgaata cgcgctgcag gcgttcgatt ccgaagccgt ggcctatcta
     1921 ttgaagccca tccgcgccga gaaactgaag gccgcgctcg ccaaggcaga gaagttgtcg
     1981 cgggcgcagc tgcagcaggt ggccgccgcc accagcgcca cggcgcatcg cacgcacatc
     2041 ggcgcgcgtg gccgtgacgg gctcaagctc attcccatcg aagaagtgtt ctgctttcag
     2101 gccgatcaga aatacacgac ggtcaaacac ctgaaaggcg aggacctcat cgaggattcg
     2161 ctcaagacgc tcgaagacga attcgccgcg tacttcctgc gcatccatcg caatgcgctg
     2221 gtgaacacga aatacctgga gcgcattgcg cgcgacgcca gcggccaaca cttcgtgcac
     2281 ctgcgcgggc tgccggttgc gctcgaagtg agccgtcgca tggcgggcga tctcaaagac
     2341 cgcttccgga tctagtcaga tccattcgat accgtacgcg acgcctcgcg agcggatctt
     2401 gtacgtcagc cgctcgcgtc ccagctccgt gccatccgcc gcccgaacga cgaactccag
     2461 cggctcgccg tctgcttcct cgaccatgaa accaaactga tatcccagga cttcggcgtc
     2521 cgggtgtacg cgacgcacat gagcgccttc cacgttgagc accgtgcctt gcgcggtgcg
     2581 ggcactcagg aacagcatcg gaaccgaact cggcgacaac tcgatgagaa cgtcgtagcg
     2641 ctcgcgcttg agtacgactt ccgtcgggaa gcccactttt ccactcttga gaggttcgcc
     2701 ctgggaatcg accgccgtcg tcgaccgctc gaggacgacg aggcccggcg tttcatcgag
     2761 taccaggtgc cgatggtcga tgcggtatgg cgcgcaggcc gtcagaagga ctgcgcctga
     2821 cacgaccgtc agcgttcgca aacgcatgga tggcacctca gcgcggccgg tcgaaaggcc
     2881 cggcgacgca caaggatgaa cccgaaaccg cgtccgcgtc caaccacgca cgcagtacgc
     2941 tgccgtcctg aagacattcc acgtcgatct cgcctggccg cgcgccgatg accgccattc
     3001 gctccgagtc tcctgcgtgg ttgcgcacgt cgacgaactg gccggggcgc gcctcgagcg
     3061 tgccttcggt gaaccgcagc tgcacacgca gcgcgcccag cggcaacgga tcgagcgcca
     3121 cgatctcgca catcgcggca ttcgaagcgc gtaccggcac ccggcgtgac tcgatggata
     3181 ggtcgctgcg cggccgcgcc tggcatagca gcacctcgcc gcgctcgacc tccgcgggcg
     3241 tgatgccggg cggaatgccc gcggggtatt cgatggctcc cgccagcagc ttcgccttgc
     3301 acgaagcgca tcggcccgcg cggcagctat agggaagcgg tatgccggcg gcgagcgccg
     3361 ccgccagcac gttctcgccg gacggcacac gaatgtcgtg accgcccggc tcgatgtgga
     3421 cgtgaaattc cgtctgcgcg ttcccgctca ggccgcctcg tccttcttct tgccgaacat
     3481 cttggcgagg acgccgccca acgcgatgac gccgatgatg atgaacttct tgcctgcgag
     3541 cagcagggca aacaacttgg cgaacaggcc cgccttcgcg gccacaccac cggcgatcag
     3601 cgcgccgagg ccgtacgccg ccacgcgatc ggtcttggag tcgaactcgg cgtagcggta
     3661 gccctcgttg aactcggcga cgtcgagcag cggacgcatg tcggtgcgca cctgctgaag
     3721 ctggtccatg gtcgcgacag cctccatcga caacacgcct tcgcggccga gcacgcgtac
     3781 gtcgtagttg agatagtggg tcttgctgtc gtcggtcgat atttccttcg cccagtagag
     3841 cttcttcgtt gcggcatcgt aacgaggcgg ctctgcccac ttgatgaggt gcatggccgg
     3901 gtagcccgct tccttgcgcg cgtcgttgtt cgactcggtg ccttccttca tgtccttgag
     3961 catgtcgtcg tagtcgatgt ccttggcgtc ggaatcgtcg acgtgccctt catcgaggta
     4021 ggtgaggaaa accgcccagc cgccctgctt cagcggattg actcccgccg ggtagatcgc
     4081 gccctgcgtg gagttgtcgg gtggattgcc ccacagctgc agcagctggc tcgcctcttc
     4141 cggggccagg taatgatatt tctcaccgag atgcagcgtg gctaccttgt taggcaacac
     4201 gatgtcgccc gtggcgaact tgagttccgg tattgccgct tcctcggcca ctggttcagt
     4261 ggagtcggcg accaactctg gttcggcctc cggcgaatcc tgcgcgaacg cgggaacggc
     4321 ggtcgcggcg caaagcagcc acgcagccgt tatattggcc agattattga caagtctcat
     4381 cacgaccctc ccaggttgtt cgacgggacg ctagcacaac acgaggccac gccatgttcg
     4441 agaaccccac gaaaccggaa atcgacgaat tcctgcgccg catccgcacc gtggccatcc
     4501 tcggcctttc cgaaacaccg ggacgcccga gccacaacgt ggcgaaggcg ctgcagaaat
     4561 tcggttaccg gatcgtcccg gtcaacccga ccgccacgga aatcctcggc gaacgcgcct
     4621 ggcc
//
LOCUS       DATDEU010000213         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_37544, whole genome shotgun sequence.
ACCESSION   DATDEU010000213 DATDEU010000000
VERSION     DATDEU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37544"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2759)
                     /locus_tag="VIV63_05255"
     CDS             complement(<1..>2759)
                     /locus_tag="VIV63_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124037.1"
                     /translation="QVVGRGSDEPLSQGKDAASLAANRRVDIVIEGSRFEANAPLELV
                     NRGSVAEDIATSGVILRGPRTEGARVTRFKPMEDKLGMGVVVDVEKLKPGNAWLAPLA
                     NATAPISVTKVAIQHLPGQSVELTVNGKKADALGFDGVDVNGPGTVAVSKWRSVDLVD
                     GENLMVARVLDKEGNEVWRAERNVHFGGGPVRAEIDVAASRLIADGRTAPVIALRMFD
                     KYGKPARPGTFAAFTVDSPYRSMWEVESLNDNQILSNGPREPQVEVRDGGLALIELEP
                     TTTTGNAIVRIRYNDRQNDEYKVWLAPAARDWILVGIAEGTAAYNTISGNMETASAAD
                     REEGFEEGGRVAFFAKGRIKGDFLLTMAYDSARKKEDARDSLKGTIEPDRYYLLYGDG
                     TEQRFEAASQNKLYLKIERRQFVALFGDFDTGFTVTELARYNRSLSGIRSDFAGDRVS
                     VSGFAARTDTGLVQDELRGDGTSGLYRLSRAPIVIGSDKLHIEIRDRFEITRVIETRE
                     LSRFIDYDLDYERGAVFFKEPVQNRDENLNPIFIVVDYEVRTGGEDETVAGVRVATRF
                     GGEKLEVAASAIQEGASAGDTRVVGGDLTWRVSDGTKVRAEVAQSQSDDPLRADSATA
                     WLVEGRHATDRIEARAWARETENDFGFGQQLTADTGTRSAGIDARYKLTETFSASGEV
                     QHQQVLTSDATRMLASAEVRMQKESWGLGAGLRHVGDEDASGDERVSDLAFLTASVDV
                     WDGRVTLRGEADAAIGGKDASVDYPARNLLGIDYHLSNDTTLFAEYEASSGDQLAADM
                     TRIGMRTRPWERTVVNSSINSQATEFGPRTFANFGLTQGFRYKENWGFDVGIDQTNTL
                     RGPDLEPLNPNAPLASGSMTDDFFAAFVGASYHQELWQMTSRLEHRNSDSEERWSSTT
                     GWYREP"
BASE COUNT          432 a          906 c          894 g          527 t
ORIGIN      
        1 ggctcgcgat accagcccgt ggtcgagctc cagcgttcct cgctgtctga attgcggtgt
       61 tcgaggcggc tcgtcatctg ccacagttcc tggtgatagg acgcgccgac aaatgccgcg
      121 aagaagtcgt cggtcatgga acctgatgcg agcggcgcat tcggattgag cggctcgaga
      181 tcggggccac gcaacgtatt cgtctggtcg atgcccacgt cgaaccccca gttctccttg
      241 tatcggaaac cctgcgtgag cccgaagttc gcgaacgtgc gggggccgaa ttcagtggcc
      301 tgggaattga tgctggagtt gaccaccgtg cgctcccaag ggcgggtacg catgccgatt
      361 cgcgtcatgt ccgctgcaag ctggtcgccc gaggaagcct cgtattcggc gaacagcgtc
      421 gtgtcattcg agaggtggta gtcgatgccc agcagattcc gcgccgggta gtcgacgctg
      481 gcgtccttgc cgccgatcgc cgcatcggcc tcgccgcgca gcgtgacgcg tccatcccag
      541 acgtcgacgc tcgccgtgag gaaagcgaga tccgagacgc gctcatcgcc actcgcgtct
      601 tcgtcgccga cgtggcgcag gccggcgccc agtccccagc tttccttttg catgcgcacc
      661 tcggcgctgg ccagcatccg ggtggcgtca ctcgtgagca cctgctgatg ctgcacctcg
      721 cccgaggcgc tgaaagtctc ggtcagcttg tagcgcgcgt cgatgccggc gctgcgagtg
      781 ccggtatcgg ccgtcagctg ctgaccgaag ccgaaatcgt tttctgtttc gcgggcccag
      841 gcgcgcgctt cgatgcgatc ggtggcgtgc ctgccttcga cgagccacgc ggtcgccgaa
      901 tcggcgcgca gagggtcatc cgactgtgac tgcgccactt cggcgcgcac tttcgtgccg
      961 tccgacacgc gccacgtgag atcgccgccc accacgcgcg tgtcgcctgc ggacgcgcct
     1021 tcctggatcg cgctggcggc cacctcgagt ttttcgccgc caaaccgcgt ggcgacgcgc
     1081 acgccggcaa ccgtttcatc ttcaccgccg gtgcgcactt cgtagtcgac gacgatgaag
     1141 atcgggttca ggttttcatc ccggttctgc acgggctcct tgaagaacac cgcaccgcgc
     1201 tcgtaatcga ggtcgtagtc gatgaagcgc gacagctcgc gggtctcgat gacgcgcgtg
     1261 atttcgaacc ggtcgcggat ctcgatgtgc agcttgtcgg agccgatcac gatcggcgcg
     1321 cgtgacagcc ggtagaggcc agacgtgccg tcaccacgca gctcgtcctg cacgagtccg
     1381 gtgtcggtgc gggccgcaaa gccgctgacc gacacgcggt caccggcgaa gtcactgcga
     1441 atacccgaga ggctgcggtt gtaacgggcg agctcggtta ccgtaaagcc cgtgtcgaag
     1501 tcgccgaaca gcgcgacgaa ctggcgacgc tcgatcttga ggtacagctt gttctgcgag
     1561 gcggcctcga agcgctgctc cgtcccgtcg ccgtagagaa gatagtaacg atcgggctcg
     1621 atcgtgccct tgaggctatc gcgcgcatct tctttcttgc gcgccgagtc gtaggccatc
     1681 gtcagcagga aatcgccctt gatgcggccc ttggcgaaga acgcgacgcg cccgccctct
     1741 tcgaagccct cttcgcggtc cgcggcgctc gcggtttcca tgttgcccga gatcgtgttg
     1801 tacgcggcgg tgccttcggc aatgccgacg aggatccagt cgcgtgcggc cggggccagc
     1861 cacaccttgt actcgtcgtt ctgccgatcg ttgtagcgga tgcgcacgat ggcattgcca
     1921 gtcgtcgtgg tcggctcgag ttcgatcagc gcgaggccgc catcgcgaac ttcgacctgc
     1981 ggctcgcgcg gtccgttgga gagaatctgg ttgtcattca gcgattcgac ttcccacatc
     2041 gagcggtacg gcgaatcgac ggtgaacgcc gcgaacgttc cgggacgcgc gggcttgccg
     2101 tacttgtcga acatgcgcag cgcaatcacc ggcgcggtgc ggccgtcagc gatcaatcgc
     2161 gatgccgcca cgtcgatttc ggcgcgcacc gggccgccac cgaaatgcac gttgcgttcg
     2221 gcgcgccaca cttcgttgcc ttccttgtcg agcacgcgcg cgaccatcag gttctcaccg
     2281 tcgacgagat cgacggagcg ccacttgctg accgccaccg tgccgggacc gttcacatcg
     2341 acgccgtcga agccgagcgc atcggccttc ttgccgttga cggtgagttc gaccgactgg
     2401 ccgggcaggt gctggatcgc gaccttggtg accgagatcg gcgcggtggc attggctagc
     2461 ggcgccagcc aggcgttacc gggcttgagt ttttcgacgt cgacgacgac acccatgccg
     2521 agcttgtctt ccatcggctt gaatcgcgtg acgcgtgcgc cttcggttcg cggaccgcgc
     2581 aggatcacgc cggacgtcgc aatgtcttcg gccactgagc cgcggttcac cagctcgagt
     2641 ggcgcattgg cttcgaaacg cgagccttcg atgacgatgt cgacgcggcg attggcggcc
     2701 aggctcgcgg cgtccttgcc ctggctcagc ggctcatcgg aaccgcggcc gacgacctg
//
LOCUS       DATDEU010000214         2955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1788332, whole genome shotgun sequence.
ACCESSION   DATDEU010000214 DATDEU010000000
VERSION     DATDEU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788332"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="ccmC"
                     /locus_tag="VIV63_05260"
     CDS             complement(<1..582)
                     /gene="ccmC"
                     /locus_tag="VIV63_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEY6124038.1"
                     /translation="MATWTWFHRLASPPHVYAFARRWTPWFGGFAAVAMCVALYGGLV
                     LAPEDYQQKDAFRMVYVHAPTAWLSLMIYTTMAIAAAVGLIWRMKVAHAAAAACAPVG
                     AWFTVAALGTGMLWGKPMWGAYWVWDPRLTAELVLLFLYFGYMGLRAGIEDPVKADKA
                     SAVLAVVGVINVPIIKFSVEWWNSLHQTATVMQM"
     gene            complement(593..1273)
                     /gene="ccmB"
                     /locus_tag="VIV63_05265"
     CDS             complement(593..1273)
                     /gene="ccmB"
                     /locus_tag="VIV63_05265"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY6124039.1"
                     /translation="MKATALTAARLVAVRDLRLAFRKPSQIIQPLMFFMIVATLFPLT
                     LTPEMSRLRDAAPGVLWIGALLSSLLALEFLFRDDAVDGTLEQFALSGQSLTWMLFAK
                     TCTHWMLTGLPLALSAPIAAAALGVSMEAMGGVVASLALGSFAMSLVGAIGAGLTLGA
                     RRGGVLLSLLTLPLAVPILIFGARATQLAISGGDIAPPMYLLGAIAVLGLTLAPLAAA
                     AAVRIGLE"
     gene            complement(1284..1901)
                     /gene="ccmA"
                     /locus_tag="VIV63_05270"
     CDS             complement(1284..1901)
                     /gene="ccmA"
                     /locus_tag="VIV63_05270"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEY6124040.1"
                     /translation="MLTVQGVHLWRGDRHVLRGLSFEGQPGKCVLLTGKNGAGKTTLI
                     RAIAGLLDPEEGQVFWRGVPARTQRDEFHSELAYLGHEPPLKGDLSARENLKYSIGIR
                     RAVTIAEIDAALLRTGALSFADRPTRMLSAGQKRRVALAGVLLANAVLWLLDEPTTNL
                     DADGQQLVRDLISEQLARHGIVVAAVHHSLALPEGQLLAVELGES"
     gene            complement(1918..2004)
                     /locus_tag="VIV63_05275"
     tRNA            complement(1918..2004)
                     /locus_tag="VIV63_05275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1968..1970),aa:Leu,seq:taa)
     gene            complement(2020..2093)
                     /locus_tag="VIV63_05280"
     tRNA            complement(2020..2093)
                     /locus_tag="VIV63_05280"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2059..2061),aa:Cys,seq:gca)
     gene            complement(2124..2199)
                     /locus_tag="VIV63_05285"
     tRNA            complement(2124..2199)
                     /locus_tag="VIV63_05285"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2164..2166),aa:Gly,seq:gcc)
     gene            complement(2242..2808)
                     /locus_tag="VIV63_05290"
     CDS             complement(2242..2808)
                     /locus_tag="VIV63_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY6124041.1"
                     /translation="MIRSVCVFCGSAPGSRPSYSRAAEEMGTALAKAGLTLVYGGGKL
                     GLMGIVADAVLKAGGRVVGVIPRMLIERECAHPSLTTQHVVTTMHERKTLMADMSDAF
                     IGLPGGMGTFDELVEIVTWAQLGLHAKPVILANIDSYFEPMYGMLDHAVREGFVTAES
                     RARWRNADSVASVMRILSKEGLTGDGNP"
BASE COUNT          612 a          970 c          903 g          470 t
ORIGIN      
        1 catctgcatg acagtcgcgg tctgatgcag cgaattccac cactccaccg agaatttgat
       61 aataggcaca ttgattaccc cgaccaccgc gagcaccgcg ctcgccttgt cggccttgac
      121 cggatcttcg atgccggccc gcaaacccat gtagccgaag tacaggaaca gcaaaaccag
      181 ctcggcggtg agccttggat cccagaccca gtatgcgccc cacatgggtt tgccccagag
      241 catgccggtg ccgagcgcgg caaccgtgaa ccacgcgccg accggcgcgc atgcggcggc
      301 ggcggcatgc gccaccttca tgcgccagat gagacccacc gcggctgcga tcgccatggt
      361 ggtgtagatc atgagtgaca gccacgcggt cggtgcatgg acgtacacca tgcggaacgc
      421 atctttctgc tggtagtcct cgggagccag caccagtcca ccgtacaggg ccacgcacat
      481 ggcaaccgcg gcgaatccac cgaaccacgg cgtccagcgc cgcgcgaacg catagacatg
      541 cggcggtgat gccagtcggt gaaaccaggt ccaggtggcc actaacgaat cctcattcca
      601 gtccgatacg caccgcggcg gccgcggcca ggggagccag cgtcaatccc agcacggcga
      661 tcgcgcctaa caagtacatc ggcggcgcga tgtctccgcc gctgatcgcg agctgcgtag
      721 cccgcgcacc aaaaatcagg atgggcaccg ccagcggaag cgtcaacaag gacagcagca
      781 cgccacctct cctcgctccc agcgtgagtc ccgctccgat ggcgccgacc aggctcatgg
      841 cgaaactgcc gagcgccagg gacgccacca ctccacccat tgcttccatc gacacaccca
      901 gtgccgcggc cgcgatgggc gccgacaagg cgagcggaag tcccgtgagc atccagtgcg
      961 tgcaggtctt cgcgaacaac atccatgtca gcgactgtcc cgataacgcg aactgctcga
     1021 gcgtcccatc taccgcgtcg tcacggaaca ggaactcgag cgccagcagc gaggataaca
     1081 acgcgccgat ccacaagaca cccggcgcgg cgtctcgcag tcgcgacatt tcgggggtta
     1141 atgtcagagg gaacagcgtg gccacgatca tgaagaacat gagtggctga atgatctggg
     1201 atggtttcct gaaagcgaga cgcaggtcac gcacggctac cagccgcgcg gcagtcagag
     1261 ccgttgcttt catggcgcgc ccatcacgat tcaccgagtt ccacggccag gagctggccc
     1321 tcgggcagcg ccagcgaatg atggacggcg gctaccacga tgccgtggcg ggccaactgc
     1381 tcgctgatca ggtcgcggac cagctgttgc ccgtccgcat cgagattggt ggtcggttcg
     1441 tcgagaagcc acaacacggc gttcgccagc aacactccgg ccagcgccac gcggcgtttc
     1501 tgcccggcgg acaacatgcg ggtgggccga tccgcgaacg acagcgcacc ggtgcgcaga
     1561 agcgccgcat cgatttcggc gatggtgact gcccggcgga tgccaatgga atacttcagg
     1621 ttctcgcgcg ccgacaaatc acctttgagc ggcggctcat gcccgaggta ggcgagttct
     1681 gaatgaaatt cgtcgcgctg cgttcgagcc ggcacacccc gccagaaaac ctgcccttcc
     1741 tcgggatcga gcagcccggc gatggcgcga atcagcgtcg tcttgccggc gccgttcttg
     1801 cccgtgagca acacgcattt ccccggctgg ccttcgaagc tcaggccgcg aagcacatga
     1861 cgatcaccgc gccacaggtg aacaccctga acggtcagca tttcatggtc atggaggtgg
     1921 tgccccgggc cggaatcgaa ccggcatggc cttgcggccg acggatttta agtccgttgc
     1981 gtctaccaat tccgccaccg gggctttgga acatggggtt ggaggctagg gtcggaatcg
     2041 aaccggcata gacggctttg caggccgctg cataaccatt ctgccaccta gccacgggcc
     2101 cgaatgatga ggaccggagg gattggagcg ggaaacgaga ctcgaactcg cgaccccgac
     2161 cttggcaagg tcgtgctcta ccaactgagc tattcccgct tcctggagag ggcgcaaatt
     2221 ctagggccag cgcccggcaa gtcaaggatt tccatctccg gtaagccctt ctttcgaaag
     2281 gattcgcatg accgaggcca cggaatccgc attgcgccag cgcgcgcggc tttccgcggt
     2341 gacgaatccc tcccgcaccg cgtgatcgag catgccgtac atgggctcga agtagctgtc
     2401 tatgttcgcg aggatgaccg gtttcgcatg taatccgagc tgcgcccagg tgacgatttc
     2461 gaccagttcg tcgaacgtac ccattccgcc cggcaggccg atgaaggcat cggacatgtc
     2521 ggccatgagc gtcttgcgtt catgcatcgt ggtcacgaca tgctgggtcg tgaggctcgg
     2581 atgtgcgcac tcgcgctcga tgagcatgcg cgggatgaca cccactaccc gcccgccggc
     2641 cttgagcacg gcatcggcga cgatgcccat gagcccgagt ttgcccccgc cataaaccag
     2701 cgtgagcccc gctttcgcca gcgccgtacc catctcctcg gccgcgcgcg agtagctagg
     2761 acgcgaaccc ggggcggaac cgcagaacac gcagaccgaa cggatcattc ggaccctcgc
     2821 agatcgggca acgcggctcg aagataggag atcgccgaga gcagcgtgag gaacgcggcg
     2881 atgatggtga gcaccaggcc gatctcgaac accggcagga tccagaggtt ccagcggaac
     2941 aacatgcagg aaaga
//
LOCUS       DATDEU010000215         3268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_913101, whole genome shotgun sequence.
ACCESSION   DATDEU010000215 DATDEU010000000
VERSION     DATDEU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913101"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..968)
                     /gene="lpxL"
                     /locus_tag="VIV63_05295"
     CDS             complement(69..968)
                     /gene="lpxL"
                     /locus_tag="VIV63_05295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HEY6124042.1"
                     /translation="MKAAYLHPKYWPTWLGIGALRLFEPLPHRLLYLLGRGLGLFIFL
                     FPTPFKRIARRNLELALPELVPEARERILREHFAGLGCALFETAMSWWSSNERIRHMT
                     LMTGLENLKAAQATGRGVLLLSAHFNSIEISCRALAARMPLNVMYRPTKNPLVGEFLH
                     SRRAVQTQRAIPRDDVRTLIKALKAGDVVWYAPDQSFRKKGAEMVKLFGIPAATNTAT
                     SRIAGMTNALVLPYFFERLPGGGYRGTIHPALDNFPTDDAVADTERFNRIVEAEVRRM
                     PDQYLWIHRRFKGLTPDYPRYYD"
     gene            complement(973..1326)
                     /locus_tag="VIV63_05300"
     CDS             complement(973..1326)
                     /locus_tag="VIV63_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124043.1"
                     /translation="MKPSLLVFALLLAAHDAGAACAEKRSRDASFVELAVPDGAIRPA
                     GVQDFGFVTDDTTVDALIAKVGPPDASQGSRTTRLIWCFADSTELWLDTPDRVSIESI
                     RHKGKTLYQRGKKKK"
     gene            complement(1332..2366)
                     /locus_tag="VIV63_05305"
     CDS             complement(1332..2366)
                     /locus_tag="VIV63_05305"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY6124044.1"
                     /translation="MSGPVVGPFGTLPDGQKVEVHTLGSADGLQAEVLTLGGTLRSLS
                     LPVRGKRIPLLLTLPDLPAYLKDTSYLGQLVGRYGNRIAGAAFELDGKQYKLTANNGP
                     NTLHGGQVGFGKYVWKVLGAGGGKNTYVKLGHRSPAGINGFPGNLDVTALISVYEDTL
                     TLAYEATTDAPTPISFTWHPYFNISGDPGRPIDEQQLMMAASHYLPISESRVPTGEIA
                     PVAGTPFDFRKAKSVRVPPPTSHPQIKLTGGFDHCWVVDQPAATSSGPIAAELYSPAS
                     GVRMRVKSNLPGLQVYGAYHLKTAYPGWHAICLEPENFPDAPNHANFPSSILKPGETH
                     RSFMSFSFSG"
     gene            complement(2363..>3268)
                     /gene="yjfF"
                     /locus_tag="VIV63_05310"
     CDS             complement(2363..>3268)
                     /gene="yjfF"
                     /locus_tag="VIV63_05310"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF008630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HEY6124045.1"
                     /translation="MYGTGLANYENFGYPQVLLNIFVDNAFLLVVAVGMTFVILSGGI
                     DLSVGSVIALTTMILATLLNAGWNPYAAMGAVLLLGAAFGALQGFLIQRFNLAPFLVT
                     LAGMFMARGCCYLISTDSIPIDDATFVAIAQARVPLGFDASLTIGGLIAIAVVVAAMR
                     LAHSSEFGRTVYAIGGGESSARLMGLSVDSTKIGVYLLSGACAALGGIVMTFYMLSGY
                     SLHAVGLELDAIAAVVIGGTLLAGGVGYVAGTLFGVLILGIIQTLIAFDGSLSSWWTR
                     IVVGALLLLFCLLQRLFEARTPGKS"
BASE COUNT          599 a          996 c         1133 g          540 t
ORIGIN      
        1 cccagcaaaa ggggtcagcc cctggtcgag aggactgacc cctttcgcca ggaaaagggg
       61 gctgccccct agtcgtaata gcgagggtag tccggcgtca aacccttgaa tcgtcgatga
      121 atccagaggt actggtcggg catgcgccgc acctcggcct cgacgatccg gttgaacctt
      181 tcggtgtcgg ccaccgcgtc gtcggtgggg aaattgtcga gcgcggggtg gatggtgccg
      241 cggtagccgc cgccgggcag ccgctcgaag aagtagggca gcaccagggc attcgtcatg
      301 ccggcaatgc gcgaagtggc ggtattggtg gccgcgggga tgccgaagag cttcaccatc
      361 tcggcgcctt tcttgcggaa gctctggtcg ggcgcgtacc agaccacgtc gccggccttg
      421 agcgccttga tgagcgtgcg cacgtcgtcg cgcgggatcg cacgctgggt ctgcacggcg
      481 cggcgcgaat gcaggaattc gcctaccagc ggattcttgg tcggccggta catgacgttg
      541 agcggcatgc gagccgccag cgcgcggcag ctgatctcga tggaattgaa atgcgccgac
      601 agcagcaaca ccccgcggcc cgtggcttgc gcggccttga ggttctccag cccggtcatg
      661 agcgtcatgt gccggatgcg ttcgttcgac gaccaccagc tcatcgccgt ctcgaacagc
      721 gcacagccga ggccggcgaa gtgttcgcgc aggatgcgct cgcgcgcctc tggcaccagc
      781 tcgggcaacg cgagctcgag gttgcgccgc gcgatccgtt tgaacggcgt cggaaacagg
      841 aagatgaaca ggcccaggcc gcggcccagt agatagagaa ggcggtgggg cagcggttcg
      901 aacagccgca acgcgccgat gccgagccag gtgggccaat acttgggatg caggtaggcg
      961 gccttcaaaa cgctacttct ttttcttgcc gcgctggtag agggtcttgc ccttgtggcg
     1021 aatcgactcg atcgacacgc ggtcgggtgt gtcgagccac agttccgtcg agtcggcgaa
     1081 acaccagatg agccgcgtgg tgcgtgagcc ctgcgaggcg tcgggcggcc cgaccttcgc
     1141 gatgagcgca tcgaccgtcg tgtcgtcggt gacgaatccg aaatcctgga ctcccgcggg
     1201 acggatggcg ccatcgggaa cggccagttc gacgaacgat gcgtcgcgcg agcgcttctc
     1261 ggcacaggcg gcgccggcgt cgtgggcggc taacagcaat gcgaaaacga gcagcgatgg
     1321 tttcatgcgc gtcagcctga gaaagagaag ctcatgaacg aacgatgcgt ctcgccgggc
     1381 ttcaggatgc tgctcgggaa attcgcatgg ttgggcgcgt cggggaagtt ttccggctcg
     1441 aggcagattg catgccatcc cggatacgcg gtcttgaggt ggtaggcgcc gtacacctgg
     1501 agtccgggca ggttggactt cacgcgcatg cgcacaccgc tggccggtga atagagctcg
     1561 gcggcgatgg gcccgctcga agtcgccgcc ggttggtcga ccacccagca gtggtcgaag
     1621 ccgccggtga gcttgatctg cggatgcgag gtgggcggcg gcacgcgtac ggacttcgcc
     1681 ttgcggaaat cgaacggcgt gccggccacg ggcgcgattt cgcccgtggg cacccgcgac
     1741 tcgctgatgg gtagatagtg gctcgcggcc atcatgagct gctgctcgtc gatggggcgc
     1801 cctggatctc ccgagatgtt gaagtaggga tgccacgtga agctgatggg cgtcggcgcg
     1861 tcggtggtgg cttcatacgc cagcgtcagc gtgtcttcgt agacgctaat gagtgcggtg
     1921 acgtccaggt ttcccggaaa accgttgatg ccggccggcg aacggtggcc gagcttcacg
     1981 taggtgttct tgccaccgcc ggcgcccaac accttccaca cgtatttccc gaatcccact
     2041 tggccgccat gcagcgtgtt cgggccgtta ttggccgtga gcttgtactg tttgccgtcg
     2101 agttcgaagg cggcgccggc gatgcggttg ccgtagcgcc ccacgagctg gcccagatag
     2161 gaggtgtcct tgagataggc gggcaggtcg gggagcgtca gcagcagcgg aatgcgtttg
     2221 ccgcgcaccg gcaaggagag gctgcgcagc gtgccgccga gcgtgaggac ctcggcctgc
     2281 agaccgtcgg cgctgccgag cgtgtgtact tcgactttct gaccatcggg cagtgtgccg
     2341 aaggggccga ccacggggcc gctcatgatt tcccgggcgt gcgcgcctcg aaaagccgct
     2401 gcaacaggca gaagagcaag agcaaagctc cgacgacgat tcgtgtccac catgagctca
     2461 gactcccgtc aaacgcgatg agggtctgga tgatgccgag aatcagcacg ccgaacaacg
     2521 tgcccgctac gtagcccacg ccgcccgcca gcagcgtgcc gccgatgacc acggccgcga
     2581 tcgcgtcgag ttcgagaccc accgcgtgca ggctgtaacc cgacagcatg tagaacgtca
     2641 tcacgatgcc gccgagcgct gcgcaggcgc cgctcagtag atagacgccg atcttcgtgg
     2701 aatcgaccga cagtcccatg agccgcgccg acgactcgcc gccgccgatg gcatacacgg
     2761 tgcggccgaa ttcgctggag tgcgcgaggc gcatcgccgc caccaccacg gcgatcgcga
     2821 tcagcccgcc gatggtcagc gaggcgtcga agcccagggg tacccgcgcc tgggcaatgg
     2881 cgacgaaggt ggcgtcgtcg atcggaatgg aatcggtgct gatgaggtag cagcagccgc
     2941 gcgccatgaa catgccggcg agagtgacga ggaaaggcgc gagattgaag cgctggatca
     3001 ggaaaccctg cagcgcgccg aaagccgcgc cgagcaacag cacggcgccc atcgccgcgt
     3061 acggattcca gccggcgttg agcagcgtgg ccaggatcat cgttgtcagt gcgatgaccg
     3121 agcccaccga cagatcgatg ccgcccgaca ggatcacgaa cgtcatgccc accgcgacga
     3181 cgagcaggaa cgcgttgtcg acgaagatgt tcagcagtac ctgggggtac ccgaagttct
     3241 cgtagttggc caggccggtg ccgtacat
//
LOCUS       DATDEU010000216         5373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1204944, whole genome shotgun sequence.
ACCESSION   DATDEU010000216 DATDEU010000000
VERSION     DATDEU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204944"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VIV63_05315"
     CDS             <1..317
                     /locus_tag="VIV63_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124046.1"
                     /translation="NLVLFYQDSKFQARVAANYLSEQYQGTFSHWSFTAPNSGLAQWQ
                     DETLYIDAGASYDFNEHFQVFLQGSNLTEEAPTNYVQWKDAHLNWNQYERVVTAGVRV
                     KF"
     gene            408..1928
                     /locus_tag="VIV63_05320"
     CDS             408..1928
                     /locus_tag="VIV63_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6124047.1"
                     /translation="MKSIVIVGGGTAGWMAAATLVERFGANRHTRITLVESPEIGTVG
                     VGEATVPHIREFLKHLKINEVDFVKRTSATFKLAIGFEGWAGEGSQFFHPFSEHGVPL
                     LGTSFQHYWVKARQLGKADVIDRYVLSSELARAGKFAVPKPGAGQGFLFFNYALHFDA
                     TLVARYLKSWSIMAGVRHVEGRVVDVNLNAESGNVESVELDGGRKVAGDLFIDCSGFQ
                     GLLIEQALQTGYDDWSHWLPCDRAVALPCEGRLAPLPYTRSIASQAGWQWRIPLQHRV
                     GNGYVFCSRYLSADEAIANLRASVEGPSLAEPRLLPFRTGIRRKIWNRNVFCLGLASG
                     FMEPLESTSIYLVQRGLQYLLGSFPQAVPNPALQENVNRRNRTHWEHLRDFIVLHYKL
                     NRREGEPFWDECRNMEIPGSLGDTMQLFRETGRLRQESIEFFRASSWLAMFAGFGILP
                     KYYSPLVDDVPEADVVSELENMAAGIAVAVAKASSHEDFIRANCLSGVPVTSAANG"
     gene            1941..2636
                     /locus_tag="VIV63_05325"
     CDS             1941..2636
                     /locus_tag="VIV63_05325"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEY6124048.1"
                     /translation="MPDFDVNPDVQVDVRRVGRDRLPVIVIDSFSMDPESLVARAIGA
                     AFIDAGSTYPGIRAPAPQGYVETLLGASASLIEEHFGAAAPEALDMCAYSMVTTPPAH
                     LRAIQRIPHFDGPDENRIAFLHYLCAPHQGGTSFYRHCATDLEQVTRNRAEAYRATVV
                     AELRTESAAQEYVVDDTRFFERIETVEAAFNRLIIYRGNALHSGDISGRTVLTDDPRR
                     GRLTINGFGFLAN"
     gene            2761..3339
                     /locus_tag="VIV63_05330"
     CDS             2761..3339
                     /locus_tag="VIV63_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6124049.1"
                     /translation="MKKLVHAALLSAMVLSACPVVHAGDAVKRGGFLAQLDLDFGGDD
                     LATLSFTNGESQNVKAGQGVAFGIGGYFRPVESVPFELQGILGYKVVLTAADNADIKV
                     TRTTLQLNGIYRFASHWYVGAGYVQHMSPELDGDGFFEDIEFDDANGFNAEIGWKWVG
                     LHYTSMDYSSDGYEDVDASHVGIRFTYRFGAN"
     gene            3417..4031
                     /locus_tag="VIV63_05335"
     CDS             3417..4031
                     /locus_tag="VIV63_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124050.1"
                     /translation="MNRKCTVAVFVAASILSGTAVAQDPAAAKVRARGGVAVQLDFSV
                     GGDELTSTANDPPYTAQAILGEGMTLSLGGFYRLSESKPWELQALVGYKGGWAVPVRG
                     GGYDADVSRWVFQLLAEYRQQDKWYFGGGLVFHGNPKWDNTAPGLADVHFDDAVGATI
                     EGGWSWMGLQCTYIEYSASGRGSLDASNCGVRFTWRFRKWGALR"
     gene            complement(4038..5054)
                     /locus_tag="VIV63_05340"
     CDS             complement(4038..5054)
                     /locus_tag="VIV63_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY6124051.1"
                     /translation="MSRITIHDVSKAAGVSIKTVSRVLNSEPNVRSETRARVLKLVEE
                     LNYHPSLSARSLAGRSSNLIGLIYQNPSANYVVDLQKGALERCHTRGYRLLVHPCEGT
                     DLSSVRDLAGLVLESHLDGVVLAPPLSDSTEVLAELQKHRIRFVRIAPTVDLDAYSCV
                     FMNDVAAAREMTEYLIALGHERIGFINGPSGHPVTEQRYLGYTLALRSHGLDVDPQLI
                     DRGEFTFDSGLAAARRLLSRATRPTAVFASNDDMAAGTIMAAHEMGLTIPSRLSVAGF
                     DDSYVAQVIWPRLTTVHQPTYEMAHQAADMLFRSLGDEAPPKSMEIPYRMVCRESTGP
                     RPAN"
BASE COUNT          989 a         1610 c         1719 g         1055 t
ORIGIN      
        1 tgaacctggt gttgttctac caggacagca agttccaggc tcgcgtggcg gccaactacc
       61 tcagcgagca ataccagggg acattcagcc actggtcatt cacggcgccg aactccggcc
      121 tcgcgcaatg gcaggacgag acgctgtaca tcgacgccgg tgcctcgtac gacttcaacg
      181 agcacttcca ggtgttcctg cagggatcga acctcacgga agaagcgcct accaattacg
      241 tccagtggaa ggatgcgcac ctgaactgga accagtacga gcgagtggtc acggccggtg
      301 tgagagtgaa gttctaaacc cgagggttcc ccaagtgctc tcgggttggc ggggcagcat
      361 ggctgccccg tctttctttt cgacgggaat gaatgcctga caatcggttg aaatcgatcg
      421 tgatcgtcgg cggaggaacc gccgggtgga tggcggccgc cacgctggtg gagcggttcg
      481 gcgccaatcg ccacacgcgc atcacgctgg tggagtcgcc ggaaatcggg acggtgggtg
      541 tcggtgaagc aaccgtgccg cacatccgcg agttcctgaa gcacctcaag atcaacgagg
      601 tggacttcgt gaagcgcacc agcgccacgt tcaagctcgc gatcggcttc gaaggctggg
      661 ccggtgaggg ctcgcagttc tttcacccgt tttccgaaca cggggtgcct ttgttaggaa
      721 cctcgtttca gcactattgg gtcaaggcgc gtcaactggg caaggccgac gtcatcgacc
      781 gctacgtgct ttcatccgaa ctggcgcgcg cgggaaagtt cgccgtaccg aagccgggcg
      841 caggccaagg gttcctgttc ttcaactacg cgctccattt cgacgcgacc ctggtcgctc
      901 gttatctgaa gagctggtcg atcatggccg gcgtcaggca tgtggagggc agggttgtcg
      961 acgtaaacct gaacgcagag tcgggcaatg tcgagtcggt ggagctcgat ggtggcagga
     1021 aagtcgcggg cgatctgttc atcgactgtt ccggcttcca gggcctgctc atcgagcagg
     1081 cgttgcagac gggttacgac gactggagcc attggctgcc gtgtgatcgc gcggtggcat
     1141 tgccctgcga aggccggctc gcgcctttgc cctacacgcg ctccattgca tcgcaggccg
     1201 gatggcagtg gcgcattccg ttgcagcacc gcgtcggcaa tggatatgtg ttctgcagcc
     1261 gctatctttc cgcggacgaa gcgattgcca acctgcgcgc gagtgtcgaa gggccgtcgc
     1321 tggcggaacc gagactgctc ccgttccgga ccggtatccg ccgcaagatc tggaaccgga
     1381 atgtcttctg tctcggattg gccagcgggt tcatggagcc gctcgagtcg accagcatct
     1441 acctcgtgca gcgcggcctc cagtatttgt taggcagttt cccgcaggcg gttccgaacc
     1501 cggcgttgca agaaaacgtg aaccgccgca atcgcacgca ctgggaacac cttcgcgatt
     1561 tcatcgtgct ccactacaag ctcaaccggc gcgaaggcga acctttctgg gacgagtgcc
     1621 ggaacatgga aatccccggc agtctcggcg acacgatgca gttgttccgc gagaccggcc
     1681 gcttgcgcca ggaatccatc gagttcttcc gcgccagttc gtggctcgcg atgttcgcgg
     1741 ggttcggaat cctgccgaag tattacagcc cgttggtcga cgatgtgcct gaagccgatg
     1801 tcgtttccga gctcgaaaac atggcggccg gcattgccgt cgcggttgcg aaagcctcct
     1861 cgcacgagga cttcatccgc gccaattgtc tgagcggcgt accggtgacg tcggccgcga
     1921 acggctgatc cccggtaacg atgcccgatt tcgacgtcaa tccggatgtc caggtggacg
     1981 tgaggcgtgt cgggcgagat cgcctgccgg tgatcgtcat cgattccttt tcgatggacc
     2041 cggaatcact ggttgcacgc gcaatcggag cggcgttcat cgacgcgggt tccacctatc
     2101 cggggatccg cgcgccggcg ccccaggggt atgtcgagac attgttaggc gcgagcgcgt
     2161 cgctcatcga agagcacttc ggcgccgcgg cgcccgaagc gctcgacatg tgcgcttact
     2221 ccatggtgac gactccgccg gcccacctgc gcgccattca gcgcattccc cacttcgacg
     2281 ggccggatga gaatcgcatc gccttcctgc actatttgtg cgcccctcac cagggcggca
     2341 cctcgtttta ccgtcattgc gccacggacc tggaacaggt gacgcgcaat cgcgcggagg
     2401 cgtatcgcgc caccgtggta gccgagctca ggactgaatc ggccgcccag gaatacgtcg
     2461 tcgacgatac gcgattcttc gagcgtatcg aaaccgtcga ggcggccttc aaccggctca
     2521 tcatctatcg cggcaacgct ctgcactccg gtgacatctc cgggcgcacg gtactcaccg
     2581 atgatccacg tcgcggacgc ctgacgatca acggattcgg cttcctggct aactaacact
     2641 cccttactac ccggctgtga cgcgtgtcat gagtcgcgcc acgagcattg acgcactcgg
     2701 tgaaaccacg agaatgcacg agcatcctgc cgggagcgca ttcacgaggg ggagggttcg
     2761 atgaagaagt tagttcacgc tgcgttgctg tccgccatgg tcttgtccgc ctgcccggtc
     2821 gtgcacgctg gcgatgccgt caaacgcggt ggcttcctgg cgcaactcga cctcgacttc
     2881 ggcggcgacg acctcgccac cttgtcgttc accaacggcg agtcgcagaa cgtcaaggcc
     2941 gggcagggcg tcgcgttcgg catcggtggc tatttccggc cagtcgaatc cgtgcctttc
     3001 gagcttcagg gaattctcgg ctacaaggtg gtcctcaccg ccgcggacaa cgccgacatc
     3061 aaggtgacac gcacgacatt gcagctcaac ggcatctatc gcttcgccag ccactggtac
     3121 gtgggcgccg gctacgttca acacatgagc ccggagctcg acggcgatgg tttcttcgaa
     3181 gacatcgaat tcgacgatgc gaacggattc aacgcggaga tcggctggaa gtgggtcgga
     3241 ctccactaca caagcatgga ttattcgagt gacggatacg aagacgtcga cgcgagccac
     3301 gtgggcatcc ggttcaccta tcgcttcgga gcgaactgat ggagaggggt ggcggcgccc
     3361 gttctgtcac cgtttcgcga gtcccggcag agaactccca accgggagtt gtctgaatga
     3421 atcgcaaatg caccgtggcc gtgttcgtgg ccgcatcgat cctgtcggga actgcggttg
     3481 cgcaggatcc tgcggcagcg aaggtgcgtg cccgaggcgg cgtcgccgta cagctcgatt
     3541 tttccgtagg cggcgacgag ctcacgagca ctgccaatga cccgccctat accgcgcagg
     3601 cgattctcgg cgagggcatg accttgtctc tgggcggctt ttatcgactg agcgagtcga
     3661 agccgtggga attgcaggcg ctcgttggat acaaaggggg ctgggcagtg cctgtgcgcg
     3721 gtggcggcta tgacgccgat gtctcgcgtt gggtgtttca gctactcgcg gagtaccggc
     3781 agcaggacaa gtggtatttc ggtggtggac tcgtattcca tggcaacccg aaatgggata
     3841 acaccgcgcc gggcctcgcc gatgtccact tcgacgacgc tgtcggtgcg acgatcgagg
     3901 gcggctggag ctggatgggt ctgcagtgca cgtacatcga gtacagcgca tcgggtcgcg
     3961 ggtcgctcga tgcgagcaac tgcggcgtgc gattcacgtg gcgcttccgc aagtggggag
     4021 cgctccgcta ggaccctcta attcgccgga cggggtccgg tggattcgcg acacaccatg
     4081 cgatacggaa tttccatgct cttgggcgga gcttcatcgc ccaacgagcg gaacaacatg
     4141 tccgccgcct ggtgcgccat ctcgtacgta ggctggtgca cggtggtcag gcgcggccag
     4201 atgacctgcg ctacatagct gtcgtcgaag cccgcaacag acagtcggct ggggatcgtc
     4261 agacccattt catgggcggc catgatggtg ccagcggcca tgtcgtcgtt gctcgcaaac
     4321 acggcggtcg gccgtgtcgc acgagacaac aatcgccgcg ccgcggccaa acccgaatcg
     4381 aatgtgaact cgccacgatc gatgagttgc gggtcgacat cgagtccgtg ggatcgcagt
     4441 gccagcgtgt agccgaggta acgctgctcg gtcactggat gtcctgacgg gccattgatg
     4501 aagccaatgc gttcatgtcc cagcgcgatg aggtattccg tcatttcacg cgcggcggcg
     4561 acgtcgttca tgaacacgca ggaatacgcg tccaggtcga ccgtgggcgc gatgcgcacg
     4621 aagcggatcc ggtgcttctg caattccgcg agcacttcgg tcgagtccga cagcggcggc
     4681 gcgagcacca cgccgtcgag gtgggattcg agcacgaggc ccgcgagatc gcgaacgctc
     4741 gatagatccg tgccttcgca cgggtgcact aacaaccggt agccgcgcgt gtggcagcgt
     4801 tcgagcgcgc ctttttgcag gtcgacgacg tagttggcgc tgggattctg gtagatgagc
     4861 ccgatgagat tcgagctgcg gccggcgagg ctgcgtgcgg aaaggctcgg gtggtagttg
     4921 agttcttcaa cgagcttgag cacccgggcg cgggtttcac tgcgcacgtt tggctcgctg
     4981 ttgagtacgc gcgagacagt cttgatagac acgcccgcag ccttggatac gtcatgaatc
     5041 gtaatacggc tcatatcaac gcccccctgt cgacgaagaa ttgtcttgtt gtcagacagc
     5101 gttgtcaaac gggtccaggt tctgcctgcc gattcgcgcc gttacgttaa ttactgggta
     5161 gaatcgccag cccaattggt tgacagcgtt gtctgcgcga gcgcagtctt cgcggtcttt
     5221 cggagcaaga tggtgacagt gaatacagcg ctttccggcg cggattccga gcgcgaacac
     5281 ttcgaccgct ccgcggtttt ggggctggtc gcggacgtcg gcggcacaca catccgcttc
     5341 ggcatcgcgg tcggcggctc gagcaccgac ggc
//
LOCUS       DATDEU010000217         6641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1351226, whole genome shotgun sequence.
ACCESSION   DATDEU010000217 DATDEU010000000
VERSION     DATDEU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351226"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1003)
                     /locus_tag="VIV63_05345"
     CDS             complement(44..1003)
                     /locus_tag="VIV63_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6124052.1"
                     /translation="MLRRTITHTASFVAIVAALTLAACSKTAEAPKEEAAPPAPAAVA
                     ETPPPAPANVKEFKIGELSAMALLDGGIEVPNDNKVFAVGRTPEEVAAVLGGASLPTD
                     KLNLTIQPLLVKSADKVLLFDTGAGGNFGPGAGKLAASFGEAGVDPQAVTDIFISHSH
                     GDHIGGLVNAQGALAFPNAKIHLSKPEWKALSGNEQMAAVVAAIKPKVEAFTPGAELV
                     PGVVKAVEIKGHTPGHSGYLISSGAESLLYVGDSMHHYVVSVQKPDWTIAFDGDAPTA
                     QKSRADLIAASAASGQRIYAVHFPFPGLGKFEKQGDHFAWVAE"
     gene            1175..2227
                     /locus_tag="VIV63_05350"
     CDS             1175..2227
                     /locus_tag="VIV63_05350"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY6124053.1"
                     /translation="MKTSELLRLRLHNQQLTNVQARKPGEVVSSLVAMQAQEYAMSRW
                     AIALRMSGSPRDADIERAFNAGEILRTHVLRPTWHFVAPEDIRWLLALTAPRVHAANA
                     YMYRQLDLDASVFKRSNAVLVRALQGEQYLTRSALQTVLAKAGVKASGPRLGYLMMNA
                     ELEALICSGPREGKQFTYALLETRVPAAKAKSRDVALAELSHRYFASRGPATAQDFAM
                     WSGLTLTEARAGIESLGAEFVHETVGRQTHVFRPANGKPRTAAGFAFLMPDYDEYGMS
                     YKDRRALLLPALARRKSRDITLAYNRMIVVDGQIVGSWRRGESAGIETAYLAPLSRTK
                     LRAVTRAIKRFTAFMR"
     gene            2290..2694
                     /locus_tag="VIV63_05355"
     CDS             2290..2694
                     /locus_tag="VIV63_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY6124054.1"
                     /translation="MVPFKIKFAPGQSLFDQVCFAAVKSILAGELKAGEAFPSVRTLA
                     LDLKIHPNTAHKVVQHLIQERWLVAQPGVGTVVAQPPKARAGDRKRLLEDEVEKLVVE
                     AMRVGAGLDEVQQAVESTWTDLRGLKAVSSDR"
     gene            2684..3583
                     /locus_tag="VIV63_05360"
     CDS             2684..3583
                     /locus_tag="VIV63_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6124055.1"
                     /translation="MIVETQSVAKRFGRFEAIEDLSLSVPEGSVFALIGPNGAGKTTT
                     IRMLMNILRPDRGDITVLGTPSRELEPRDFARIGYVSESQKLPEGLGLSQYFDYLRSL
                     YPSWDTSLENDLRQQFELPPSRKIKHLSHGMRMKTLLVGALAFRPQLLVLDEPLSGLD
                     TLVRDEVVNGLLQQAADTTILISSHELTEIETFTTHVAFMQNGRLLLQEAIETLQTRF
                     RTVLVTLSAEKALPDPLPAGWLLPEIEGHRLRFVASNHEDDQQLFRQLTQHFGAVRME
                     CEPMPLRAIANALMQQRKRNLER"
     gene            3580..6084
                     /locus_tag="VIV63_05365"
     CDS             3580..6084
                     /locus_tag="VIV63_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124056.1"
                     /translation="MNFRILAAIIRKDVLSLAPIVALVALLYLADALIERLHLLPAWT
                     LYKSPAMLAALVVLILSVFQTDSPASVNDDWLCRPVPKRELIAAKLALLLGTVYLPHA
                     VGTLVADLSLGFSWAESLLDAVLVRYDPDLMLLPVLLFTAIVTRTLVQGFGVLFAIFL
                     FLIVLPTPFVRPPSPLTPGLREELWFSGMPWLAWTPAVLTSLMLLAVGFWLMYWRRHV
                     TAARSLMALSVSISFLFLVLPMALLPLKTIFAVQTALSPTSPADTAHIFLRSPRACFP
                     AARRADLSTNAAFDAAWEKEALQDVGPNSIAFLTHIEARGLPLDWRVKLTYVQANYSA
                     GGATLFSLRPAQYITDKGGGGLLTHEWMLPESAIRGLRGAQSRLELEYALSLLRPIEH
                     LVPADGKRHALPGLGFCSAVVDEPGNRIEVDCFSAFTRTAQISAELIDIPASRVYGSA
                     DFAPTSVKWLFGKHVRLDIGSPRLARHDSIKITSWEATNYFAKSLTLPGILGADLETC
                     PLPSSERDGFQKASWRDAAPHEASSIRVDEGVQLEVLDFGGTGSPILLLPGLGATAHS
                     YDELAPLLAKKHRVFAMTRRGTGYSSKPDFGFDTARLGQDVLQVMDALSLPKVLLVGH
                     SIAGDELTWLGGKHPERFSALVYLDAAYDRSGDPKSPDALRLRELNRTLPPEPPRPPQ
                     AMLSFDAMTKMLLERGHVRIPEGELISLHRVNDPLFAGTPSIEPRTQQAISAAIQAPD
                     YAAVKIPALAVYAIEDPHKPLPPWYDPNDKELAANLAERARLVDAMKRESIELFRRGV
                     EKGQVLELTNASHYLIQSNQQEVLSAIEQFVAGLGL"
     gene            complement(6081..>6641)
                     /locus_tag="VIV63_05370"
     CDS             complement(6081..>6641)
                     /locus_tag="VIV63_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY6124057.1"
                     /translation="MNDATQWKPWLIRDQRFVSERPDVASWKSAPLEKAVHIMGAPEV
                     ELFAATTGTDSDWVVKLIDVYPNDVPEVAAQGAKPGMAGYELPIGIEIFRGRYLKSFA
                     VPAPLKAGKVERYSWKLPDVDHVFLPGHRIMVQIQSSLFPLYDRNPQTYVENIMYAKP
                     GDYKKATQSVWFGGATATAVVLPVVP"
BASE COUNT         1210 a         2163 c         2021 g         1247 t
ORIGIN      
        1 tcagggccgg agacatccgt ctccggccct ttttgtcgac taactattca gcgacccacg
       61 cgaaatggtc gccctgcttc tcgaacttgc ccagtcccgg gaacgggaag tgcaccgcgt
      121 agattcgctg gccgctcgcg gccgacgcgg cgatgaggtc ggcgcggctc ttttgcgccg
      181 tgggcgcatc accgtcgaac gcaatggtcc aatccggctt ctgcaccgag accacgtagt
      241 gatgcatcga gtcgccgacg tagagcagcg attccgcacc cgaggagatc aggtaacccg
      301 agtgacccgg cgtgtggccc ttgatctcga ccgctttcac gacgcccgga accagttctg
      361 cgcccggcgt gaacgcctcg accttgggct tgatcgccgc cacgaccgca gccatctgct
      421 cgttgcccga cagcgccttc cactccggct tcgacaggtg gatcttcgcg ttcggaaacg
      481 ccagcgcgcc ttgcgcgttc acgagtccgc caatgtgatc gccgtgcgaa tgcgagatga
      541 agatgtcagt gacggcttgc ggatcgacac ccgcttcgcc gaatgacgct gccagcttgc
      601 ccgcgcctgg cccgaagttg ccgcccgcgc cggtgtcgaa cagcaacacc ttgtcggcag
      661 atttcacgag caacggctgg atggtgagat tcagcttgtc agtgggaaga ctggcaccgc
      721 cgagcactgc ggccacttcc tcgggcgtac ggccgacggc aaacaccttg ttgtcgttcg
      781 gcacttcgat gccaccgtcg agtagcgcca tcgcggagag ttcgccgatc ttgaattcct
      841 tgacgttcgc gggggcaggc ggcggggttt cggccacggc tgccggcgcc ggtggcgcgg
      901 cctcttcttt cggcgcttcc gcggtttttg aacacgctgc cagagtcagg gcagccacga
      961 tggcaacgaa cgacgccgta tgagtaatgg ttctacgcag catgagcctc tcctgaatcg
     1021 gggtggctaa cttaataggc tttgaagcgc cttcgcaacg cctctaacgg gagagcgcaa
     1081 tcagcgtttc tgactccgtg atacagggct tcatctaacc cgtattccga ggggcggcgc
     1141 aaccgcctgc cgagtctggc aaagttcgta cgccatgaag acgtctgaat tgctccgcct
     1201 ccggttgcac aaccagcagc tcaccaacgt ccaggctcgg aagcccggtg aagtcgtgag
     1261 ctctctcgtg gcgatgcagg cccaggaata tgcgatgtcc aggtgggcaa tcgccctgcg
     1321 aatgtccgga tcgccgagag acgccgacat cgaacgggca ttcaacgccg gagaaatcct
     1381 gcgcacgcat gtgttgcgcc ccacctggca cttcgtcgcg ccggaggaca ttcgctggtt
     1441 gttagcgttg accgcacctc gcgtgcacgc agccaatgcg tacatgtatc ggcaactcga
     1501 cctggacgca tcggttttca aacgcagcaa tgccgtgctg gttcgcgcgc tccagggcga
     1561 gcagtacctg acacgctctg cactgcaaac cgtgctggca aaagcgggag taaaagcgtc
     1621 gggaccgcgc ctgggctacc tcatgatgaa cgcggagctc gaagccctca tctgcagcgg
     1681 cccgcgggaa gggaaacagt tcacttacgc gttgctcgaa acgcgcgtgc cggccgccaa
     1741 ggcgaagagc cgcgacgtcg cgctcgcgga gctcagtcac cgctacttcg ccagccgcgg
     1801 accggccacc gcccaggact tcgccatgtg gtccgggctg acgctgaccg aagcccgcgc
     1861 gggtatcgaa tcgctgggcg ccgagttcgt tcatgaaacc gttggcaggc agactcatgt
     1921 gtttcgtccc gccaacggga aaccgcgtac tgctgccggc ttcgcgttcc tcatgcccga
     1981 ctacgacgaa tacggcatga gctacaagga tcgccgggca ttgctcctgc cggcgttggc
     2041 acggcggaaa tccagagaca tcacgctggc ttacaaccgc atgatcgtcg tcgatggtca
     2101 gatcgtcggc tcctggcgtc gtggcgaatc ggccggaatc gaaacggcct atctcgcgcc
     2161 gttgagccgc acgaagcttc gtgcggttac ccgcgccatc aaacgtttca ccgcattcat
     2221 gcgctgagca tcgggagttt acgcgcccac tcctatgtgt cacagtattc atgtgacacc
     2281 ggaggacgca tggttccctt caagatcaag ttcgcgcctg gccaatccct cttcgatcaa
     2341 gtgtgcttcg cggccgtcaa atccatattg gccggcgaac tgaaggccgg tgaggcgttc
     2401 ccttccgtcc gcacgctcgc gctggatctc aagatccatc cgaacaccgc acacaaggtg
     2461 gtgcagcacc tgatccagga acgctggctg gtggcgcaac ccggtgtagg aacggtggtg
     2521 gcgcagccac cgaaagcgcg cgcaggagat cgcaagcgcc tgctcgagga cgaggtggag
     2581 aagctcgtgg tcgaagccat gcgcgtcggc gcgggcctcg atgaagttca gcaggccgtg
     2641 gaaagcacgt ggaccgacct tcgcggcctg aaagcggtgt ccagtgatcg ttgagacgca
     2701 aagcgtcgcc aaacgtttcg gtcgattcga agcgatcgaa gacctgagcc tgagcgtgcc
     2761 ggaaggctcg gtgttcgcgc tgatcggccc gaatggcgcc ggcaagacca ccaccattcg
     2821 catgctcatg aacatcctcc gccccgaccg cggcgacatc accgtgctgg gcacgccgtc
     2881 acgcgagctc gagccccgcg atttcgcgcg catcggctac gtgtcggaga gccagaaact
     2941 gccggaaggg ctcgggcttt cacaatattt cgactacctc cgtagccttt atccctcctg
     3001 ggacacgagc ctcgaaaacg atctgcgcca gcaattcgag ctgccaccgt cccgcaagat
     3061 caagcacctc tcgcatggca tgcgcatgaa gacgctgctg gtaggtgcgc tcgcgttccg
     3121 cccccaacta ctggtgctcg acgagccgct gagcggcctc gacaccctgg tgcgcgacga
     3181 ggtcgtcaat gggctgcttc aacaggccgc ggacaccacg atcctgattt cctcgcatga
     3241 actcacggaa atcgaaacgt tcaccacgca cgtggccttc atgcagaacg gcaggctcct
     3301 cttgcaagaa gcgatcgaaa ccctgcagac gcgattccgg actgtactgg tgacgctttc
     3361 ggccgagaag gcgttgccag atccgctgcc cgctggctgg ctgctgccgg aaatcgaagg
     3421 gcaccggctg cgtttcgtcg cttctaatca tgaagacgat cagcagctgt tccggcaact
     3481 gacgcaacac ttcggcgctg taagaatgga atgcgagccc atgccgctac gcgcgatcgc
     3541 gaacgcgctc atgcaacagc gcaaacggaa tctggagcga tgaacttcag gatcctcgcc
     3601 gccatcatcc ggaaagatgt gctgagcctc gcgcccatcg tggcgctcgt tgccctgctc
     3661 tacctcgccg acgcgctcat cgagcggctg cacctccttc ctgcgtggac gttatacaaa
     3721 tcaccggcga tgctggcggc gctggtcgtg ctgatcctgt cggtgtttca aaccgattcg
     3781 cccgcgagcg tcaacgacga ctggctgtgc cgcccggtgc cgaagcgcga gctgatcgcc
     3841 gccaagctgg cgctgctgct gggaactgtc tatctacctc acgccgtggg caccttggtc
     3901 gcggacctga gtctcgggtt ttcgtgggcg gagtcgctcc tggacgcggt gttggtgcgg
     3961 tacgacccgg acctgatgct gctgcccgtc ctgctgttca cggccatcgt cacgcggacg
     4021 ctcgtccagg gcttcggcgt gctgttcgcg atcttcctgt tcctgatcgt gctcccgacg
     4081 cctttcgtgc ggccgccgag tccgttgacc ccggggctcc gcgaagagct gtggttctcc
     4141 ggcatgccgt ggctggcttg gacgccggcc gtgctcacgt cactgatgct gctcgccgtc
     4201 ggattctggc tgatgtactg gcgcaggcac gtgaccgcag cgcgatcgct gatggctctc
     4261 tcggtcagca tttcgttcct gttcctggtg ttgccaatgg cgctgttgcc attgaaaacc
     4321 atttttgccg ttcagaccgc gctcagcccg acgtcgcccg ccgatacggc ccacattttc
     4381 ctgcgtagtc cacgcgcctg ctttccggcc gcgcgccgcg cggacctgtc gacgaatgcg
     4441 gcgttcgatg ccgcgtggga aaaggaggcg ctgcaagatg tcggaccgaa ctccatcgcg
     4501 tttctcaccc acatcgaagc gcgaggcttg ccgctcgatt ggcgcgtgaa gttgacctac
     4561 gtgcaagcga actactctgc cggcggcgcg acgctcttct ctctccggcc cgcccaatac
     4621 atcaccgaca agggcggcgg cggattgctg acgcatgaat ggatgttgcc cgagagcgcg
     4681 atacgaggac tgcggggcgc gcaatcccgg ctcgagctcg agtatgcgct gtcgctgctg
     4741 aggccaatcg agcacctggt gcccgcggat ggcaagcgcc acgcgttgcc gggcctcggg
     4801 ttttgcagcg ccgtggtcga tgagccaggc aatcgcatcg aggtcgactg cttcagcgcg
     4861 ttcacgcgta ccgcgcagat cagcgcggag ctgatcgaca tccccgcgag ccgcgtctac
     4921 ggtagtgcgg acttcgcccc gacttccgtg aaatggcttt ttggcaaaca cgtgagactc
     4981 gacattggct cgccacgact cgcgcgccac gacagcatca agatcacatc atgggaagcg
     5041 acgaactatt tcgcgaagtc cctgacgttg cccggcatcc tcggcgcaga cctcgagact
     5101 tgtccgttgc cctcgagcga gcgcgacggc tttcagaaag cgagctggcg tgacgccgcg
     5161 ccgcacgagg cttcctcgat acgcgtcgat gagggcgtgc agctcgaagt gctggatttc
     5221 ggcggcacgg gctcgcccat cctgctgctg cccggccttg gcgcgacggc gcacagctac
     5281 gatgaactcg ccccgttgct cgcgaagaag catcgcgtgt ttgccatgac gcgccggggt
     5341 actggctact cgagcaagcc ggatttcggt ttcgacaccg cgaggctggg acaagacgta
     5401 ctgcaagtga tggatgcgct gagcctgccg aaggtgttgc tcgtgggtca ttccattgcc
     5461 ggcgatgagc tgacctggct gggcggaaaa catcccgagc gcttcagcgc gctcgtctac
     5521 ctggacgcgg cttacgatcg ttctggcgat cccaaatccc ccgacgcatt gcggctgcgt
     5581 gaactcaacc ggacgctgcc acccgaacca ccacgcccgc cgcaggccat gctgagtttc
     5641 gacgcgatga caaagatgct gctcgagcgc ggacacgtgc gcattccgga gggcgaactc
     5701 atctctttgc accgggtgaa cgatccgctt ttcgccggca ctcccagcat cgaaccgcgc
     5761 acacagcagg cgatcagcgc cgcgatccag gcgccggact atgcggcggt gaagatcccc
     5821 gcattggcgg tctatgcgat cgaggatccc cacaaacccc ttccgccctg gtacgacccg
     5881 aacgacaagg aattggccgc aaacctggcg gagcgcgccc ggctcgtcga cgccatgaag
     5941 cgcgagagca tcgagctatt caggcgaggt gtcgaaaaag gccaggtgct ggagctaacg
     6001 aacgcctcgc actacctcat ccagtcgaat cagcaggaag tattgagcgc catcgagcaa
     6061 tttgtcgccg gcctcggtct ctagggaacg accggcaaca ccaccgcggt cgccgtcgcg
     6121 ccgccaaacc acacgctctg cgtcgccttc ttatagtcgc ccggcttcgc gtacatgatg
     6181 ttctcgacgt aggtctgcgg attgcgatcg tagagcggga acaggctcga ctggatctgc
     6241 accatgatcc ggtgacccgg caggaacacg tggtcgacgt cgggaagttt ccagctataa
     6301 cgctcgacct tgccggcctt gagcggcgcc ggcacggcga agctcttcag gtaacgtccg
     6361 cgaaagatct cgatgccgat cggcaattca taacccgcca tgcccggctt cgcgccctgc
     6421 gccgcgactt ccggcacgtc gttcggatag acgtcgatga gtttcacgac ccagtcgctg
     6481 tccgtgccgg tcgtagcggc aaacaactcg acctcgggcg cgcccatgat gtggacggcc
     6541 ttctcaagcg gtgcgctctt ccaggacgcc acgtcgggcc gctccgacac gaaacgctga
     6601 tcgcggatga gccacggctt ccactgcgtc gcgtcgttca t
//
LOCUS       DATDEU010000218         5093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_767620, whole genome shotgun sequence.
ACCESSION   DATDEU010000218 DATDEU010000000
VERSION     DATDEU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767620"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..541)
                     /locus_tag="VIV63_05375"
     CDS             complement(137..541)
                     /locus_tag="VIV63_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124058.1"
                     /translation="MKLQLRIRVDLAPGCSVGPGKVALLEAIERDGSLSVAARTLGLS
                     YRRAWNLLADLNRSFTDPVVATAVGGSRGGGAQVTEFGRSLIAAFRALEKGALRLASA
                     RMRRFKVDPRSRVEPAARRRVSGRRGKAGKNR"
     gene            complement(547..1068)
                     /locus_tag="VIV63_05380"
     CDS             complement(547..1068)
                     /locus_tag="VIV63_05380"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY6124059.1"
                     /translation="MKLQENWRDIRRLFTQVFQTSLHYSMATVTPDGMPHVTPVGSLL
                     LREPGHAIYFEEFTQRMPANFRDNQNVCVLAVRSGRWFWLSSLLRGGFRSPPAIRLHG
                     TVGPHRAATEEEIAAWQRRVGRLRFTRGHRMMWANMRTVRDVYLTRADGIYLGEMTRE
                     CWAHFGAGDAATG"
     gene            complement(1065..1625)
                     /locus_tag="VIV63_05385"
     CDS             complement(1065..1625)
                     /locus_tag="VIV63_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124060.1"
                     /translation="MNKAVNISAIAAAVFLAIAGQALAHEPKVDGCDAASPDIALELA
                     VMRSPAIPVTAVSGKDSPYPELQLDKHYAISLIAQSDLEFPAKPRRSARNQAPRGGIL
                     RFEVPAAGRYRISTTSRHWIDVVDGKSVVTSASHEGPECEILHKIVEFDLPAARPLTL
                     QISGRDDAIIGLAITLSPTAPKQDSK"
     gene            1707..2495
                     /gene="modA"
                     /locus_tag="VIV63_05390"
     CDS             1707..2495
                     /gene="modA"
                     /locus_tag="VIV63_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6124061.1"
                     /translation="MRIPRPSLRWIMPCALALSFMGTARSEEPQKPELLVLAAASLTN
                     VLGELAPAWEKTSGVRVKLSFAASSVLARQIEAGSKADVFVSADQEWMDYLQARRLIA
                     PDSRVNLVRNRLVLIAPADSPIRLEIKPGFDLAGALAGGRLATGDPDTVPVGRYARAA
                     LTSLGVWDQVADRLVRADNVRGAMLFVSRGEAPLGIVYSTDAQIDNKVRTVATFPDHT
                     HPAITYPAAATAIAGPESAAFLAFLGGSEAAPVWTKYGFLEIAK"
     gene            2492..4087
                     /locus_tag="VIV63_05395"
     CDS             2492..4087
                     /locus_tag="VIV63_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY6124062.1"
                     /translation="MKCSLTLRAAVALALGLGLSPAFADDAALKAEVDALRAAISQQR
                     AALDAQAKLLEAQQAQLEALTKQLGQPKVASTEPQKIAAPEAPKLTLNNNRPTITSAD
                     GKSSFAVRANVQLDGAMYGEDKEGPLATDYRRGSVGATANRENNAARDFSDGFYFRRA
                     RFGVEGTISRDFNYRLLLELGGSGTEGPTRINDAWIAYTGLAPFTFQLGAFSPSANMD
                     DGTTPEDLVFLERASSSELSRSLGGADGRIGLALKANGKRWMSAFTITTRTVNDAEVF
                     DGQLAAVGRAGGLVATGSDFNVHLGASGTYVFSPPDQGSSAAPPRHPLRFRDRPEIRV
                     DSVRLIDTGTLDAAHASVLGMEFGANWKAFYLQGEHFWYDVERRGAPALPDPDFTGYY
                     VQGSWMLTGESRRYNPVTGSFQNPRPKDVFSRGGGAGAWELAARYSNMNLNFQEGAEG
                     TANVPGSVRGGEQNVLTLGVNWYLNPNFKVMMNYLLINVERLNPAGPGNLAPFGPAPA
                     TPPVGVEIGQDLNVIALRTQYSF"
     gene            complement(4091..>5093)
                     /locus_tag="VIV63_05400"
     CDS             complement(4091..>5093)
                     /locus_tag="VIV63_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY6124063.1"
                     /translation="TEHVHGARTLSAYCRPDNLLRIDDVVEIMFKCAKALHYAHTRGV
                     IHRDIKPSNIMLTQDSDVRIIDFGIALVADSEISRIEGIAGSPSYMSPEQVQSLELTH
                     HSDLYSLGAVMYELLTGIRPFRAGNLVKLLHQIVYATPPPIHTQRKDVPEELEEIVAQ
                     AMLKTPDKRQKSGADFAAELTRVHQRLREQNARIDQQEQFDVLRRLRFFHEFSHAEIW
                     EVLRASAMQDYPAGEEIVKEGEMDDRFYILVNGRCQVERHGKALGFIDSGDCFGETSY
                     VRGARRTATIKAETSVSVLRVSSTLLEQVSAACQLRFNRVFLRALIGRLQGNDQQTE"
BASE COUNT          903 a         1692 c         1615 g          883 t
ORIGIN      
        1 gcagcagcac gagaagtcgg tcggcgtagc cacgcgccac ctgcgttcga atgaaccgac
       61 atgtcgcgtt cgccggcaat atccctcctg atcgcatgcc tttcctgcgc ggggctcgca
      121 cacgcgggcg cgcggctcag cggttctttc cagcctttcc acgccggccg ctgacgcggc
      181 ggcgagcggc cggttccact ctcgagcgcg ggtccacctt gaagcggcgc atacgcgcgg
      241 aagcaagccg cagcgcacct ttctccagcg cgcgaaacgc cgcgatcagg gaacggccga
      301 actccgtgac ttgagcgccg ccgcctcgtg agcctcccac cgccgtggcc accaccggat
      361 cggtgaatga gcgattgaga tccgcgagca ggttccacgc gcgtcggtag ctcaagccca
      421 gagttctggc tgcgaccgag agcgacccgt cgcgctcgat ggcttccagc aacgcgacct
      481 tgcccggccc caccgagcag cccggggcga gatcgacgcg gattcgcagc tgcaacttca
      541 tcgcagctac cccgtcgccg cgtcgccggc tccaaaatgc gcccagcatt cacgagtcat
      601 ttcgccgagg taaatgccgt ccgcacgtgt cagatacaca tcccgcaccg tgcgcatgtt
      661 ggcccacatc atccggtggc cgcgcgtgaa acgcagccgg ccgacccggc gctgccacgc
      721 cgcgatctct tcttccgtgg ccgcgcgatg agggcccacc gtgccgtgaa gtcgaatcgc
      781 cggcggcgat ctgaaaccgc cgcgcaacag cgacgagagc cagaaccagc gtccactgcg
      841 caccgccagt acgcagacgt tctggttgtc acggaagttg gccggcatgc gctgcgtgaa
      901 ttcctcgaag tagatagcgt gtcccggctc gcgcagcagc agcgacccca cgggagtgac
      961 atgcggcatg ccgtccggcg tgacggtcgc catcgagtaa tgcagcgagg tctgaaagac
     1021 ctgggtgaag aggcggcgga tgtcccgcca gttttcctgc agcttcattt cgaatcctgt
     1081 ttcggcgctg tgggagacag ggtgatcgcg aggccgatga tcgcatcgtc cctgccgctg
     1141 atctgcagcg tcaacgggcg cgctgccggc aggtcgaact cgacgatctt gtggagaatc
     1201 tcgcactcgg gaccttcgtg cgatgcggag gtcaccaccg acttgccatc caccacgtct
     1261 atccagtggc gcgatgtggt ggaaatccgg tagcgacccg cggccggaac ttcgaatcgc
     1321 agaatgcctc cgcgcggcgc ctggttacgc gccgaacgcc tgggtttggc cggaaattcc
     1381 aggtcgctct gcgcgatcag gctgatggcg taatgcttgt cgagctggag ctccgggtag
     1441 ggagaatctt tcccggatac ggccgtcacc ggaattgccg gtgaacgcat gacggccagt
     1501 tcgagagcga tgtccgggga ggctgcatcg caaccgtcca ctttcggttc atgcgccagg
     1561 gcctgcccgg cgattgccag aaacacggcg gcggcgatcg cagagatgtt gacggccttg
     1621 ttcatcggta gactccgatc cgttcgtaat gcttgacggt atatagcgga aactggatca
     1681 caacgacgcc cggagtgccc gctctcatga gaattcccag accttcgctg cggtggatca
     1741 tgccatgcgc gctcgcgctg tcgttcatgg gtactgcccg ttccgaggaa ccgcagaagc
     1801 ccgagctgct ggtcctggcc gccgcctcgc tcaccaacgt gctcggtgaa ctcgcgccag
     1861 cctgggaaaa gacttccggc gtcagggtga aactgtcgtt tgccgcaagc tcggtgctcg
     1921 cgcggcagat cgaagcgggc agcaaggcag acgtctttgt ctccgccgat caggaatgga
     1981 tggactacct gcaggcgcgc aggctcatcg cgccagactc acgcgtcaac ctcgtgcgaa
     2041 accggctggt gctcatcgcg cccgccgaca gtcccattcg gctggagatc aaaccggggt
     2101 tcgaccttgc cggcgcgctc gccggcggcc ggctcgccac gggcgatccc gacaccgtgc
     2161 ccgtgggcag gtacgcgcgt gcagcgctca cttcgttagg cgtgtgggat caggtggcgg
     2221 accgcctggt gcgcgcggac aacgtgcgcg gcgcgatgct gttcgtttcg cgcggcgaag
     2281 caccgctcgg catcgtttat tcgactgacg cacagatcga caacaaagtg cgaaccgtcg
     2341 ctactttccc cgatcacacg catccggcga ttacctatcc tgcggcggca accgccattg
     2401 cggggccgga atcggccgcg tttctcgcct ttctgggcgg cagcgaggcc gcgcccgtct
     2461 ggacgaaata cggattcctg gagatcgcga aatgaagtgc tcgctcaccc tgcgcgccgc
     2521 cgtggcgctg gccttgggcc ttggcctgag tcccgcattc gccgacgacg ccgctctcaa
     2581 ggccgaagtc gacgctttgc gcgccgccat cagccagcag cgagcggctc tcgatgcgca
     2641 ggccaaacta ctggaagcgc agcaggcgca gctcgaagcg ctcacgaagc agctcggcca
     2701 gccgaaagtc gcctcgaccg agccgcagaa aatcgccgcc ccggaagcgc cgaaactcac
     2761 gctcaacaac aaccggccca cgatcacttc cgccgacggc aagtcgtcgt tcgccgttcg
     2821 cgccaacgta cagctcgacg gggcgatgta tggcgaggac aaggaaggcc cgctcgccac
     2881 ggattaccgc cgcggctcgg tcggcgcgac cgcgaatcgc gagaacaacg cggcgcgaga
     2941 tttcagcgac ggcttctact tccgccgcgc ccgtttcggt gtcgaaggca ccatctcgcg
     3001 cgacttcaac taccgcctgc tgctcgagct cggcggcagc ggcactgaag gtcccacgcg
     3061 tatcaacgac gcgtggatcg cgtacacggg tctcgcgccc ttcacgtttc agctcggcgc
     3121 attttcgcca tccgccaaca tggacgacgg caccacgccc gaggatctgg tgttcctcga
     3181 acgcgccagt tcatccgaac tctcgcgctc gctgggtggc gccgatggcc gcatcggctt
     3241 ggcgctcaag gccaacggca agcgctggat gagcgccttc acgatcacca cacgcaccgt
     3301 gaacgatgcg gaagtcttcg acggccagct tgcggcggtc ggccgcgccg gcggactcgt
     3361 cgccaccggc agcgacttca acgtgcatct cggcgcctcg ggcacctatg tgttcagtcc
     3421 gccagaccag ggctcgtccg cggctccgcc gcgtcacccc ctgcgtttcc gcgaccgccc
     3481 cgaaatccgc gtcgacagcg tgcgtctcat cgataccgga accctggacg ccgcgcacgc
     3541 cagcgtgctc ggcatggaat tcggcgcgaa ctggaaggcg ttctatctac agggcgaaca
     3601 cttctggtac gacgtcgaac gccggggcgc gccggccctg cccgatccgg atttcaccgg
     3661 ctactacgtg cagggaagct ggatgctcac gggcgagagt cgccggtaca acccggtcac
     3721 gggctcgttc cagaatccgc gcccgaaaga tgtgttctcg cgcggaggcg gcgccggcgc
     3781 gtgggaactc gccgcgcgct acagcaacat gaatctgaac ttccaggaag gcgccgaagg
     3841 caccgccaat gtgcccggca gcgtacgcgg aggcgagcag aacgtgctca cgctcggcgt
     3901 gaactggtat ctcaacccga acttcaaggt aatgatgaat tacctgctga tcaacgtgga
     3961 gcgcctgaat cccgcgggcc ctggcaacct cgcgcctttt ggccccgcgc ccgcgacgcc
     4021 gccggtcggc gtcgagatcg gtcaggatct caacgtcatc gcgttgcgaa cgcaatacag
     4081 tttttgaggt ttattcggtc tgctgatcgt tgccctgcag gcgcccgatg agcgctcgca
     4141 ggaacacgcg attgaagcga agctgacagg cggccgacac ctgttcgagc aacgtcgagc
     4201 tcacgcgcag cacggacacg gaagtctcgg ccttgatggt cgcggtgcgg cgggcgccgc
     4261 gcacgtagct ggtctcaccg aagcagtcgc cgctgtcgat gaaacccagc gccttgccat
     4321 ggcgttccac ctggcaccgg ccgttgacga gaatgtagaa gcgatcgtcc atctcgcctt
     4381 ccttgacgat ctcctcgccc gccgggtagt cctgcatcgc gctggcgcgc agcacttccc
     4441 agatttcggc gtgggagaac tcatggaaga agcgcagacg gcgtaacacg tcgaactgct
     4501 cctgctgatc gatgcgcgcg ttctgctcgc gcagccgctg atgaacgcgc gtgagttcgg
     4561 cggcgaagtc ggcgcccgat ttctggcgtt tgtccggcgt cttgagcatc gcctgcgcga
     4621 cgatctcttc gagctcttcg ggaacgtcct tgcgctgcgt gtggatcggc ggcggcgtcg
     4681 cgtagacgat ctggtgcagc agcttgacga ggttgccggc gcggaacgga cggatgccgg
     4741 tcagcagttc gtacatgacc gcgcccagcg aatacagatc cgagtggtgc gtgagttcga
     4801 ggctctgcac ctgctcgggc gacatgtagg aaggcgaacc ggcgatacct tcgatacgcg
     4861 agatctcgga atcggccacc agcgcgatac cgaagtcgat gatgcgcacg tccgaatcct
     4921 gcgtgagcat gatgttcgac ggtttgatgt cgcggtggat cacgccacgc gtatgagcgt
     4981 agtgcaacgc cttggcgcac ttgaacatga tctcgacgac gtcgtcgatg cgcagcagat
     5041 tgtcgggccg gcagtacgca gaaagagtcc gcgctccgtg tacgtgctcg gtg
//
LOCUS       DATDEU010000219         5415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1497295, whole genome shotgun sequence.
ACCESSION   DATDEU010000219 DATDEU010000000
VERSION     DATDEU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497295"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1019)
                     /locus_tag="VIV63_05405"
     CDS             complement(102..1019)
                     /locus_tag="VIV63_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEY6124064.1"
                     /translation="MNSRGWSTLPFLMVGAMLVAGATHAADTTRQLRDDSGESVTLSS
                     APCRIVSLAPGTTAMLFAAGAAHCMVGTIAHSNEPAEAAKVPVIGDAETLDFEQLIAL
                     RPTVVVVAVDVVQRVRIDRIRALGIPVYQVHVTRLAQMPESLRGLGKLAGTEAAAHGK
                     ASELAAELAALDTHYRNRAPVRVLYQIWDRPIYTIGGRHVINDALTLCGAVNVFADLG
                     TAAPAVTREAAILRNPELILASAPPASAQEWLAEWRKFPALAAVRDRQLVSYVDERMD
                     RMGPSVIAATGNLCGVIDKARAARAAAGS"
     gene            complement(1016..1261)
                     /locus_tag="VIV63_05410"
     CDS             complement(1016..1261)
                     /locus_tag="VIV63_05410"
                     /inference="COORDINATES: protein motif:HMM:NF042692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6587 family protein"
                     /protein_id="HEY6124065.1"
                     /translation="MAQQIIVAAIVVIAFAASAWKLAPARRRLQALMALDRWAARHPR
                     LAGWRERALKPRILRAAGTGCGGCAANVGVRPHHPPR"
     gene            complement(1271..3145)
                     /locus_tag="VIV63_05415"
     CDS             complement(1271..3145)
                     /locus_tag="VIV63_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEY6124066.1"
                     /translation="MDAVVSTPSLRLALVGVPNCGKTALFNRLTGSRQKVANYPGVTV
                     ERREGERVALDGRVLRILDLPGTYSLTPTTLDEAITRDVLLGRVADAPAPELVVCVVD
                     AVHLPIGLRLALEVKRLGLPMVLALNRMDVAKRRGVHIDTARLSAELGVPVLPTIAVK
                     RGGADALIEAIQPARWEGHRQVAAPAWQESTAEDAEQTQSDLRGVLAAVGYNAPRENR
                     WFERIDSILLHPVWGLLVLAAVLFVMFQAVFSWAEAPMDWIDGGLARLGEFTAASLPE
                     GPLSSLLVNGVIAGAAAVLVFLPQILILFFFILALEDSGYLPRAAYLLDRMMGGVGLS
                     GRAFIPLLSSFACAIPGIMAARTIPSWRDRLTTIMIAPLMTCSARLPVYALLISALIP
                     ARDVGPFNLQGLVLFALYLAGIVGALAVAWVMKHFAGVRQYSPLMLELPDYQWPHAGN
                     LLLGLWERVKIFLSRVGGIILALMIVLWFLSSYPAPPDGATGPPIQYSFAGRLGAMLE
                     YIFAPIGFNWQISIALVPGLAAREVAVSALGTVYAMSATGDDVAGRLEPVIASTWSLA
                     TACSLLVWYVFAPQCLSTLAVVKRETNSWRYPLIMAGYLFALAYVFSFITYRAVLAFT
                     "
     gene            complement(3176..3523)
                     /locus_tag="VIV63_05420"
     CDS             complement(3176..3523)
                     /locus_tag="VIV63_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEY6124067.1"
                     /translation="MKSPDSNAISRPLAELRRGEAAIVAGLAEVTGFDDGLGSGAILL
                     ARLRDLGFVAGARCQVVARMWPVGDPLVVRIGGSTFALRRAEAAAVRVTPLEAPPVTR
                     SQMAPKDTSAALA"
     gene            3653..4291
                     /locus_tag="VIV63_05425"
     CDS             3653..4291
                     /locus_tag="VIV63_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6124068.1"
                     /translation="MNYIAERRLEEKERRRAEIVDAAEAAGREVGLDALTMDDVARRA
                     RLSRALLYVYFQDRSDLMFGLAERAMGMLHARFVEAAERHRTGLEQVSAMGRAYVAFS
                     QEFPVLFDALARCELQSSEPTEGSPSEQACAVGGDRLQATLVASIETGVRDGSIRGDV
                     GSPILMSVTLWGFMHGIIQLTTTKAHALTHHGVTAKALIDHAISMITRDLKS"
     gene            4359..>5415
                     /locus_tag="VIV63_05430"
     CDS             4359..>5415
                     /locus_tag="VIV63_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6124069.1"
                     /translation="MRVAGLCLGLALTALPLPSFAQGVVAEPGRPLAAVIDEYVREGL
                     RANLSLRAQSLEVERSEAALDGARARYFPEVGLDARYSRSEGGRTIELPLGQALNPAY
                     QTLNDLLIAQGQAPQFPAVQDETIAFLREREQDTRITLRMPLVHLAIPAAVRAQRELL
                     GASEYAQVALARQLKRDITVGYLHWLSAVRTRGIVDASVVLLNENLRVNDSLHRNGKI
                     TQDQVLRARAELLAVTQQSREAQNLAAQAQSNLNFLLNRPLDTALENADVAAEVSART
                     QALADLRTAALANRPELAELTHLTRASEAQVNIARADRWPTLSLGADGGIQGEEYEFG
                     RGSNYATVSLLLNWTFFD"
BASE COUNT          993 a         1789 c         1835 g          798 t
ORIGIN      
        1 cggccgatgc aaaaagattc ctgccgccgc gggggacgca agatacccgc cacgggcaat
       61 ccggtacaac cgtccggtca ggcggggccc ggaactggcc ttcagctacc ggcggcggcc
      121 cgggcggcgc gcgccttgtc gatgaccccg cacagattgc cggtcgcggc gatgaccgag
      181 gggcccatgc ggtccattcg ctcgtcgaca taactcacca gttgccggtc gcgcaccgcg
      241 gccagcgccg gaaacttgcg ccactcggcc agccactctt gcgccgaagc cggcggtgcc
      301 gaggcgagaa tcagttcggg attgcgcagg atcgccgcct ctcgcgtgac cgcgggcgcc
      361 gcggtgccca gatccgcaaa cacgttgacc gcgccgcaca aggtgagcgc gtcgttgatc
      421 acgtgccgtc cgccgatggt gtagataggc cggtcccaga tctggtacag cacgcgaacc
      481 ggcgcgcggt tgcggtaatg ggtatccagc gccgcgagtt cggcggcgag ttcactggcc
      541 ttgccgtgag ccgcggcttc cgtgcccgcg agttttccca ggccgcgcag cgattccggc
      601 atctgcgcca ggcgcgtcac atgcacctgg tagacaggaa tgccgagcgc gcgaatgcga
      661 tcgatgcgca cgcgctgcac gacgtccacg gccaccacga ccacggtggg acgcagcgcg
      721 atgagttgct cgaagtcgag tgtctccgcg tcgccgatca ccgggacttt ggccgcctcg
      781 gcgggctcgt tgctgtgcgc gatcgtgccc accatgcaat gcgccgcgcc ggcggcaaac
      841 aacatggcgg tggtgcccgg ggcgagcgac acgatgcgac acggtgcgga actcaacgtc
      901 accgactcac cggagtcgtc gcgaagttgc cgggtggtgt cagcggcgtg cgtcgcgccc
      961 gcgacgagca tcgccccgac catgagaaac ggaagcgtgc tccagccgcg agaattcacc
     1021 gcggtggatg atgcggccgg acgccgacat tcgcggcgca gccaccgcaa cccgtgcccg
     1081 cggcgcgcag gatgcgcggc ttcagcgcgc gttcgcgcca gccggccagt cgcggatgac
     1141 gtgcggccca gcggtcgagc gccatcagcg cctgcagacg ccttcgggca ggggcgagtt
     1201 tccacgcact ggccgcgaag gcgatgacga cgatggctgc gacgatgatt tgttgcgcca
     1261 tggagtgacc ttacgtgaag gccaggacgg cgcgataggt aatgaacgaa aacacgtacg
     1321 ccagcgcgaa cagatagccc gccatgatga gcgggtaacg ccacgagttg gtttcgcgtt
     1381 tcaccaccgc gagcgtcgac agacactggg gcgcgaagac ataccagacg agcaacgagc
     1441 aggcggtagc caggctccag gtgctcgcga tcaccggttc gagacggccc gccacgtcat
     1501 cgcccgtggc cgacatggca tacacggtac cgagcgcgct cacggcgact tcgcgcgcgg
     1561 cgagtcccgg caccagcgcg atggagatct gccagttgaa gccgatgggc gcgaagatgt
     1621 attcgagcat ggcgccgagg cgcccggcga agctgtactg gatgggcggg ccggtggcgc
     1681 cgtcgggtgg cgccgggtag ctcgacagga accacaacac gatcatcagc gcgaggatga
     1741 tgccgccgac gcggctcagg aagatcttca cgcgctccca caggcctaac aacaggttgc
     1801 cggcgtgtgg ccactggtaa tcgggcagct cgagcatcaa cggcgaatac tggcgcacgc
     1861 ccgcgaagtg tttcatgacc cacgccaccg cgagggcgcc gacgatgccg gccaggtaca
     1921 gcgcgaacag gaccaggccc tgcaggttga acggcccgac gtctctcgcg gggatcagcg
     1981 cactgatcag caacgcgtac accggcaggc gcgccgaaca ggtcatgagc ggcgcgatca
     2041 tgatggtggt gagccggtcg cgccagctcg ggatcgtgcg cgcggccatg atgccgggaa
     2101 tggcgcaggc gaaactggag agcagcggga tgaacgcgcg gccggacagg ccgacgccac
     2161 ccatcatgcg gtccagtaga taggccgcgc gcggcaggta gccggagtcc tcgagcgcga
     2221 ggatgaagaa gaagaggatg aggatctgcg gcaggaacac cagcaccgcg gcggcgccgg
     2281 cgatgacacc gttgaccagc aggctcgaca acggaccttc gggcaggctc gcggcggtga
     2341 attcgccgag ccgcgcgagg ccaccgtcta tccagtccat cggcgcttcc gcccagctga
     2401 acacggcctg gaacatcacg aacaggaccg cggccagcac cagcaggccc cagacgggat
     2461 gaagcagtat cgaatcgatg cgttcgaacc agcggttttc gcggggcgcg ttatagccca
     2521 cggccgccag cacgccgcgc agatcgctct gcgtctgttc ggcgtcttcg gcggtggatt
     2581 cctgccacgc gggcgcggca acctggcgat ggccttccca gcgcgcgggt tgaatggctt
     2641 cgatgagcgc atcggcgcca ccgcgtttca cggcgatggt cggcagcacc ggtacgccga
     2701 gttcggccga caggcgcgcg gtgtcgatgt ggacaccgcg gcgtttggcg acatccatgc
     2761 gattgagcgc cagcaccatg ggcaggccca gccgtttcac ctcgagagcg aggcgcaggc
     2821 cgatgggcag gtgcactgca tcgaccacgc acaccacgag ttccggagcc ggcgcatcgg
     2881 cgacacggcc gagcaacaca tcgcgcgtga tggcttcgtc gagtgtcgtc ggtgtgaggc
     2941 tgtaggtgcc cggcaaatcg aggatgcgca gcacgcgccc atcgagcgcg acgcgttcgc
     3001 cttcacgtcg ttcgacggtc acgcccgggt agttagcgac cttctggcgg ctgccggtca
     3061 ggcgattgaa gagcgccgtc ttgccgcagt tggggacgcc gaccagcgca agacgcagcg
     3121 acggggtgga aacgacggcg tccatggcgt ggccgactgc tcgagcccga aagctctaag
     3181 cgagtgctgc gctggtgtcc ttgggcgcca tctgcgaacg cgtcacgggc ggagcttcga
     3241 ggggcgtgac gcgcacggca gccgcttcgg cgcggcgcaa tgcgaacgtc gaaccgccga
     3301 tacgaaccac gagcggatcg ccgaccggcc acatgcgcgc gacgacctgg caacgggcgc
     3361 cggccacgaa tccaagatcg cgcagccggg ccagcaggat ggcgccactg ccgagcccgt
     3421 catcgaaacc cgtgacttcg gcaagtccgg ccacgatggc ggcttcgccg cgacgcagtt
     3481 cggcgagcgg tcgggaaatg gcattcgaat caggcgattt cacggcccgc agtatggagt
     3541 cagggcccct cggatgtaaa tgcgatgggt tcgcatttgc gaatagtcgc gacttgcaca
     3601 catctctact aattgacact acgtcaggga gaagcctagg ctctcggccc atatgaatta
     3661 catcgctgaa cgcagactcg aggaaaagga gcgccgtcgc gcggagatcg tcgacgccgc
     3721 ggaagcggcg ggccgggaag tcggtctcga cgctctcacg atggacgacg tggcgcgccg
     3781 cgcacgtctg tcgcgcgctc tcttatatgt gtacttccaa gaccgctccg atctcatgtt
     3841 cgggctggcc gagcgcgcca tgggcatgct gcatgcgcgc ttcgtcgaag ccgccgagcg
     3901 gcatcgcacg ggcctcgaac aggtgagcgc catgggccgc gcctatgtcg ctttttcgca
     3961 ggaattcccc gtgcttttcg acgcactcgc acgctgcgag ctgcaatcgt cggaaccgac
     4021 cgaaggctcg ccgtccgagc aggcgtgtgc ggtcggtggc gaccggctgc aggccacctt
     4081 ggtggcgtcg atcgaaaccg gcgtacgtga cggcagcatc cgcggcgacg tgggctctcc
     4141 cattctcatg agcgtcacct tgtggggttt catgcacgga atcatccagc tcaccaccac
     4201 caaggcgcac gcgctgacac accacggcgt cacggcgaaa gcgctcatcg atcacgccat
     4261 cagcatgatc acccgagatc tgaagagctg accccgaagg acttcgagac atggattgtt
     4321 tgcgcccacg attagacatg cgtccagcga ctaaacaaat gagagtcgcg gggctgtgct
     4381 tgggtctggc gctgaccgcc ctgccgctgc cgtctttcgc ccagggagtg gttgccgagc
     4441 cgggccgccc gctggcggcg gtcatcgacg aatacgtgcg cgaaggcctg agggccaacc
     4501 tgtccttgcg cgcgcaatcg ctcgaagtcg aacgatccga agccgccctc gacggcgcgc
     4561 gtgcgcgcta cttccccgaa gtgggcctgg acgcacggta ctcgcgatcc gaaggcggcc
     4621 gcacgatcga actgcctctg gggcaagcgc tgaacccggc ctaccagacg ctcaacgatc
     4681 tgttgatcgc gcagggccag gcgccgcagt tcccggcggt gcaggacgag accatcgcgt
     4741 tcctgcgcga gcgcgaacag gacacgcgca tcacgctgcg catgcccctg gtgcacctgg
     4801 ccatcccggc ggcggttcgc gcccagcgcg agttgttagg cgccagcgaa tacgcgcagg
     4861 tggcgctggc gcgccagttg aagcgcgaca tcacggtggg ttacctccac tggctctcgg
     4921 cggtacgaac ccgcggcatc gtcgatgcga gcgtcgtgct gctcaacgaa aacctgcgcg
     4981 tcaacgactc cctgcatcgc aacggcaaga tcacccagga ccaggtgctg cgcgcgcgcg
     5041 cagagttgct ggccgtcacg cagcagtctc gcgaagcgca gaacctcgcg gcgcaggcgc
     5101 agagcaacct caacttcctg ctgaaccgcc cgctcgacac cgcgctcgag aatgccgacg
     5161 tggcggccga ggtcagcgca cgtacccagg cgctggccga tctgcgcacc gcggcgctgg
     5221 ccaaccggcc cgaactcgcc gaactcacgc acctgacccg cgccagcgag gcgcaggtga
     5281 acatcgcgag agccgatcgc tggccaacgc tgtcgctggg cgccgatggc ggcatccagg
     5341 gtgaggaata cgaattcggc cgcggcagca attacgccac ggtatcgctg ctgctcaact
     5401 ggactttctt cgatg
//
LOCUS       DATDEU010000220         4432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1497430, whole genome shotgun sequence.
ACCESSION   DATDEU010000220 DATDEU010000000
VERSION     DATDEU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497430"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VIV63_05435"
     CDS             complement(<1..965)
                     /locus_tag="VIV63_05435"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEY6124070.1"
                     /translation="MVALIFLAVIAAVFTVVVALEGGDPAFERRPKGLLTWIASGNWP
                     AKIGGGLMVVGVGALLRYAAINFDIPATFKIALGIAAAAALGFGSFLTSAHPQRRVIS
                     LALGGAAFGVAYLTAYSAFALFGYLPTLQGLGLLVLTAVGAGVFAVSRSAVSLAVLSM
                     VGAFMAPAFAVDDPGPGVVYGYYVAISVLTLAMVSFRGWRPLIHLSFLFTLAGSAFFA
                     WTADYFVPENAPVLYPLLTMLVAVHVAMPLVERRWTRGTFVESLDTLYLLALPLVAAI
                     TALAIAPSRASLAMQLCWYGAIWIAAAGWLYSQRREGMATHAIIGL"
     gene            1217..3118
                     /locus_tag="VIV63_05440"
     CDS             1217..3118
                     /locus_tag="VIV63_05440"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY6124071.1"
                     /translation="MLVACGGGGGSGSSSSSSGGANTPPVANAGANQTVNSGATVTLN
                     GTASSDANGTIASYGWTQTAGTAVILANPTTSQPTFTAPNVAAATTLTFSLVVTDNSA
                     AVSPAAVTSVTVNPSVAGNTTVTGNVTFARVPFSTTGNRGLDYANPVQQPARGVIIRA
                     VDAAAPATVLATGSTDNLGNYSLSVNGNTNITIQVVARMTRTAAPVWDVRVQDGVSAS
                     APYSYTEPGSFNSSAGVAHNVAIPTGISAAGVASGDRPSGPFAILDTVYKGFETVVAV
                     APGTSFPALIVDWGSQATGTFFSSGPTQYISLVSTLAADTDEFDQHVIAHEFGHYIEF
                     NFSRADNIGGPHGLGDKLDARVAFGEGFGYAFAAIVLNDPDARDSAKNNTLFYSTGFN
                     IEFNPPANPANAVDGDYGCWCSESSVWSILWDLHDNAADANDNLSIGFAPIWQVLTGA
                     QRTTPAYTTIFPFITALKIARPADAAAINTLVAAQNIDATNIDAFGTGETHVPANVAA
                     AGALPLFTTITTGAPVVVRNVNDAGVHNKLGSRRFLRFTPAASGSVTISLTTSNPNSP
                     DPDFTLQRAGTYLLIEDDPPSTPQAETGTLTVTAGTTYVLDVYDCANGCDSAAANGSG
                     DFDLTVTIN"
     gene            3127..3777
                     /locus_tag="VIV63_05445"
     CDS             3127..3777
                     /locus_tag="VIV63_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124072.1"
                     /translation="MRTNKSGAVLLALLLAGCDKPAPENTPVPPSDVPGQPAPAVPDA
                     NPPMDVPAPDAAPAPPPAPDAPPPAEPSVVPKPASSKEPPVDSMLAAIPSAKMSVAVD
                     LRYSFDGPVLPNQPVNVHLAAIPRNAGAKLTVSVQEAPGLQVAASELTVQKTSASGVY
                     RQQFSLTRMAPETKQLSVLVILETAAGSGFGYFTVPLESSPVDGTNPQKQDSVKQR"
     gene            3812..>4432
                     /locus_tag="VIV63_05450"
     CDS             3812..>4432
                     /locus_tag="VIV63_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124073.1"
                     /translation="MARSNTRKIRLGVSSLLALAICALPQANAACVGTQTGDLAEIEE
                     RAFRDPASALPELANTITSTADMPPTRRAALHAIAAEAARQLGFSRQSITHADAGLAL
                     LPAGDMSDLAVRMRTVRAVVSTNVGGIDAATVELTRVIEAVRDRPLALGCVLRDRGWL
                     NFRDGNPDQALDDLLRSYSLLRRNASREEAMVAAGRLSMAQFSVRDY"
BASE COUNT          834 a         1566 c         1361 g          671 t
ORIGIN      
        1 aggccgatga tggcgtgggt ggccatgccc tcgcgtcgct ggctgtacaa ccagccggcc
       61 gcggcgatcc agatcgcgcc gtaccagcac aattgcatcg cgagcgatgc gcgcgatggc
      121 gcgatcgcca gcgccgtgat cgccgcgacc agcggcagcg ccagtagata gagcgtgtcg
      181 agcgattcga cgaatgttcc gcgcgtccag cgccgctcga ccagcggcat ggccacgtgt
      241 accgcgacca gcatggtcag cagcggatac agcaccggcg cattttccgg aacgaagtag
      301 tccgcggtcc aggcgaaaaa ggcactgccc gcgagcgtga acaggaaact gagatggatg
      361 agcggccgcc agccgcggaa cgacaccatg gccagcgtga gcacgctgat cgccacgtag
      421 tagccgtaca cgacgcccgg acccggatcg tcgaccgcga acgccggcgc catgaacgcg
      481 cccaccatcg agagcaccgc gagcgacacg gcgctgcgcg agaccgcgaa cacacccgcg
      541 ccgacggccg tgagcaccag caggccgaga ccctgcagcg tgggcagata tccgaacagc
      601 gcgaaggcgc tgtaagccgt gaggtaggcg actccgaatg ccgccccgcc gagcgccagc
      661 gagatgacgc ggcgctgcgg atgcgccgag gtcaggaacg agccgaagcc gagcgccgcg
      721 gccgcggcga ttcccagcgc gatcttgaaa gtcgcgggaa tgtcgaagtt gatcgccgcg
      781 taccgcagca atgcgccgac accgacgacc atgaggccgc cgccgatttt cgcgggccag
      841 ttgccgctgg cgatccaggt gagcagacct ttcggccgcc gctcgaatgc cgggtcgccg
      901 ccctcgaggg cgaccaccac cgtgaacacc gccgcaatca ctgccaggaa tatcaacgcc
      961 accatgaatt gtccctcccg taggtagcga gacgcatggt gcccgtttgc cgggtttgtt
     1021 ggtggaaatc ctgaaagtgc cggattccgc gtcccccggg tatcaagggc cgtgaccatc
     1081 cgcacacttc gcgcacaccc gggtccctga cgccgtattg agacgccttg gcactcccct
     1141 cacgcttcgc gcgccggtat aagacggtat gcgccaatcc tcgccaggcc taactcttgc
     1201 cggcatagcc gccctgttgc tcgtcgcctg tggcggtggt ggcggaagcg gaagcagcag
     1261 tagcagcagc ggaggcgcca atacgccgcc cgtggccaac gcgggtgcga accagaccgt
     1321 gaattccggc gcgaccgtca cgctcaacgg caccgccagc agcgacgcca acggcaccat
     1381 cgccagctac gggtggacgc agacagcggg caccgccgtc attctcgcca accccaccac
     1441 ctcgcagccc acgttcacgg cgccgaacgt tgccgccgcg acgacgttga cgttctcgct
     1501 ggttgtcacg gacaacagcg cagcggtcag ccccgccgcc gtcaccagcg tcacggtcaa
     1561 tccgtccgtc gctggaaaca cgaccgtgac cggcaacgtg actttcgcga gagtgccgtt
     1621 ttccacaacc ggaaaccgcg ggctcgacta cgcaaacccg gttcagcagc ccgcgcgcgg
     1681 agtgatcatc cgcgccgtcg atgcggcagc gcctgcgacc gtgctcgcca ccggcagcac
     1741 ggacaacctc ggcaactact cgctgtcagt caacggcaat accaacatca cgatccaggt
     1801 tgtcgcgcgc atgacgcgca ccgccgcgcc cgtatgggac gtgcgtgtgc aggatggtgt
     1861 ttcggcaagc gctccttatt catacaccga accgggcagc ttcaactcca gcgccggcgt
     1921 cgcgcacaac gtggctattc ccaccggcat cagtgccgct ggagtggcca gcggtgatcg
     1981 cccgtccggg ccgttcgcga ttctcgacac ggtttacaag ggattcgaga ccgtcgtcgc
     2041 cgtcgcgccg ggcaccagct ttccggcatt gatcgtcgac tggggatcac aagccaccgg
     2101 cacgttcttc tcgtccggcc ccacgcagta catctcgctg gtgtccacgc tggccgcgga
     2161 caccgacgaa ttcgatcagc acgtcatcgc gcatgaattc ggccactaca tcgaattcaa
     2221 tttctcgcgc gccgacaaca tcggcggacc acacggcctc ggcgacaagc tcgatgctcg
     2281 agtcgccttc ggcgagggat tcggttacgc gttcgcggcc atcgtgctca acgatcccga
     2341 cgcgcgcgac agcgcgaaga acaacaccct cttctattcg accggattca acatcgaatt
     2401 caatccaccg gccaacccgg cgaacgccgt cgacggcgat tacggctgct ggtgcagtga
     2461 gtcgtcggtg tggtcgatcc tctgggacct ccatgacaac gcggccgatg ccaacgacaa
     2521 cctgtccatc ggcttcgcac cgatctggca ggtgctgacg ggtgcgcaac ggaccacgcc
     2581 cgcgtacacc acgatctttc cgttcatcac cgcgctgaag atcgcgcggc cggccgatgc
     2641 ggccgccatc aacacgctcg tcgcggcgca gaacatcgac gcgaccaaca tcgacgcgtt
     2701 cggcaccggc gaaacacatg tgccggcgaa tgtcgccgcg gcaggcgcgt tgccgctgtt
     2761 caccaccatc accacgggcg cgccggtggt cgtgcgcaat gtcaacgacg caggcgtgca
     2821 taacaagctc ggcagtcggc gattcctgcg gttcacgccg gcggccagtg gctccgtcac
     2881 gatttcactc acgacatcga acccgaattc gccagatccg gacttcacgc tgcaacgcgc
     2941 cggcacttac ctgctcatcg aagacgaccc gccgtcgacg ccacaggcgg aaaccggcac
     3001 gctcactgta accgcgggca cgacctacgt actcgatgtc tacgattgcg ccaacgggtg
     3061 cgacagcgca gcggcgaatg gcagcggcga cttcgacctc acggtcacga tcaattgagg
     3121 cgatacatgc gaactaacaa gtctggcgcc gtgttgttag ccctgctgct cgcgggttgt
     3181 gacaaacccg cgccggagaa caccccggtt cccccgtccg acgtgccggg acaacctgca
     3241 cccgccgttc ccgacgcgaa tccgcccatg gacgtgcccg cccccgatgc cgcgccagcg
     3301 cctccgcccg cgcccgatgc gccgccgccg gccgagccgt ccgtggtccc caagccggca
     3361 tcgtcgaagg agccacccgt cgattccatg ctcgcggcca ttcccagcgc gaagatgagc
     3421 gtcgcggtcg acctgcgtta cagcttcgac gggccggtgc tgccgaacca gcccgtcaac
     3481 gtgcatctcg cggcgatacc ccgcaatgcg ggagccaaac tgaccgtgag cgtgcaggaa
     3541 gcgcctggat tgcaggtcgc cgccagcgag ctgacggtgc agaaaaccag tgcatcaggc
     3601 gtgtatcgcc agcaattttc gctgacccgg atggcacccg agacgaagca gttgagcgtg
     3661 ctggtcatct tggaaacggc tgcgggatcg ggcttcgggt acttcacggt tcccctggaa
     3721 tccagtccgg tcgacggaac aaatcctcag aaacaggatt cggtaaaaca gcgctgaccg
     3781 cactgcagcg cgggcgcgta gactacgcgc cgtggcccgt tcgaatacca ggaagatccg
     3841 gctcggggtc agcagcctgt tggcgctggc catctgtgca ttgccgcagg caaacgccgc
     3901 gtgcgtgggg acgcaaaccg gcgatctcgc cgagatcgaa gagcgtgcct tccgcgatcc
     3961 cgccagcgcg cttccggaac tcgccaacac catcacttcc acggccgaca tgccgccgac
     4021 gcgtcgcgcg gcgttgcacg ccatcgccgc agaagccgcg cgccaactcg gcttcagccg
     4081 ccagagcatc acacacgcgg atgccggcct cgcgctgctg cccgccggtg acatgtccga
     4141 cctcgcggtc cgcatgcgca cggtacgcgc cgtggtcagc acgaatgtcg gtggcatcga
     4201 cgccgccacg gtcgaactca cgcgcgtcat cgaagcggtt cgcgaccggc cactggcgct
     4261 cggctgcgtg ctgcgcgatc gcggctggct gaatttccgc gatggcaatc ccgaccaggc
     4321 actcgacgac ctgttgcgct cgtactcgct gcttcgccgg aacgcgagcc gcgaagaggc
     4381 catggtggcc gcgggccgcc tgtcgatggc ccagttcagc gtgcgcgact at
//
LOCUS       DATDEU010000221         6165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2081024, whole genome shotgun sequence.
ACCESSION   DATDEU010000221 DATDEU010000000
VERSION     DATDEU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081024"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VIV63_05455"
     CDS             <1..1083
                     /locus_tag="VIV63_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124074.1"
                     /translation="NFEGRVHPEIKMNFLASPPLVVAYALAGTMDVDLQTDVLGTGPD
                     GKPVLLANIWPSDKEVADAVHATIDSGMFRKSYANVFAGEANWQAIEIPLGKIYDWKE
                     ESTYVRNPPYFEGMTMTPRPVTDVTGARCLALLGDSVTTDHISPAGDIAKTSPAAKYL
                     VSKGVQPLDFNSYGARRGNHEVMMRGTFANIRLRNLLAPGTEGGVTVHVPTGEQMSIF
                     DAAMRYKQDSTPLVILAGKEYGTGSSRDWAAKGTMLLGVRAVIAESFERIHRSNLIGM
                     GVLPLMFPVGKGALSLGLTGNEVFEITGLDNAEAKTVQVVATPPKGSPIKFEARVRID
                     TPKERDYYRHGGILQYVLRQLASKAA"
     gene            complement(1157..2122)
                     /locus_tag="VIV63_05460"
     CDS             complement(1157..2122)
                     /locus_tag="VIV63_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY6124075.1"
                     /translation="MKRRVAFLLFPDFQLLDAAGPISAFEIAERYAPGSYELRVIAKQ
                     PGAVTSTSGASMNAVSLSRPASVDTLVVVGGEGTRAASKDARTVKFVAACGAQARRVA
                     SVCSGAYILAATGLLDGRAATTHWTRSNDFAQKFPRVKLDADRIFVKAGKFWSSAGIT
                     AGIDLSLALIAEDVGEGVARKVAQQLVVYYRRPGGQSQFSTLLELERADGKFAPLLDY
                     VRTHLRERLSVDELAAKACMSPRNFARLFQGETGTSPARAVMRLRAETARARIESGGA
                     SVQEIARDCGFGDPERMRRAFISLFGRPPSALRMGTVPPILSKRG"
     gene            2225..2917
                     /locus_tag="VIV63_05465"
     CDS             2225..2917
                     /locus_tag="VIV63_05465"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY6124076.1"
                     /translation="MTAPFHVVFALFPRITQLDFTGPYEVLTRLPGARCVLASVQGGD
                     LEVDPVMRFSGLRRLDSVDECDLLCVPGGFGTVAAVEDQAYLAALRRLGAKARYITSV
                     CTGSLLLAAAGLLQGKRAACHWAWRDKLADFGVTQDPARVVRDGIVFTGGGVTAGIDM
                     ALEVMAEIGGRDLAETVQLAIEYAPAPPFESGRPELAREPVIRAARARLDSMRVERDA
                     AIGRAAAALNAA"
     gene            complement(2924..4309)
                     /gene="creD"
                     /locus_tag="VIV63_05470"
     CDS             complement(2924..4309)
                     /gene="creD"
                     /locus_tag="VIV63_05470"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEY6124077.1"
                     /translation="MAERFRFIPPLVAKALVIGFLIVLLLVPLGQVEDLVGERVGMRQ
                     TAAMRVSESLGGAQTTAGVLLAIPVDTTRLVVEQTAAGRETQRTEISRNVLYVLPDTL
                     KVSANAETDYRTVGLYQTPVYKAQVQVEGEFVNGDFAQLLQPRQGREVKWGEARLLVL
                     NSESRALRAVDDLEVAGERLPVSADGYAGSAGISAAVPAAAVRESASIPFRMKLTLVG
                     TSQLNFLPLARKAEISIKSLWPHPKFEGAPAPLDSEITDKGFSARWSVLEINRNFGQS
                     WYDDQVRPGEPAEMSFAQSRVGVAFYEPVDIYQRNYRAVHYAVLLIVITFLTFFLWEH
                     VSGIPIHGMQYLMVGLALALFYLLLLALSEHMTFDRAYAVSAGALVALITVYLTGVLR
                     RVWLAVGAGAGLAMLYTMLYWILRSEDYSLLMGSLLLFGVLATLMIATRRIDWANVTP
                     RMRAKELGEES"
     gene            4420..5067
                     /locus_tag="VIV63_05475"
     CDS             4420..5067
                     /locus_tag="VIV63_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY6124078.1"
                     /translation="MAIAFFDLDGTITHRDTLFPLVLRLLAWPRPWRLLKLLRVAPAA
                     VRFAFDHDRAALKQSLLRATLRGASRDELHKACEVFVTDTIAHRCFADALAAVRRHRD
                     AGHYLVLMSASVDFYVPEFGRQLGFDQVISTGVAWNGDRLDGTLTTANRRGDEKARCL
                     RALVDGRRDSEVFAYGNAASDLPHLRLTRHGLLVNGSVAARREAASVGVACAEWI"
     gene            5269..>6165
                     /locus_tag="VIV63_05480"
     CDS             5269..>6165
                     /locus_tag="VIV63_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY6124079.1"
                     /translation="MARAACAALACLFSSAALASPPLNVQRYSLEEGLSQQSVNSIVQ
                     DSEGFMWFGTEDGLNRFDGYEFQQLRHDRGDNETLPNGWISSLVASDEGLWIATDGGG
                     VVFRSALTGKLDAPALLRDTPDLQRVRTLARDRLGRLWIGTRDSGIGIFDVRSKELHR
                     IRHSPSQPNSLSDNSIFSIIHLRSGDTLIGTATGLDRLTASNLDVSHIAWPVELVPAG
                     QPLRVRALTETPDGMVWVGTDAGLGRYDARSARWRVYRETPADSPNHLSSLPDNRIQS
                     LLLDSQGRLWVGMLRGLAWFDSA"
BASE COUNT         1065 a         2097 c         1930 g         1073 t
ORIGIN      
        1 aacttcgaag gccgcgtgca tccggaaatc aagatgaatt tcctcgcctc gccgccactc
       61 gtcgtcgcct acgcgctcgc gggcaccatg gacgtcgatc tccagacgga cgtgctcggc
      121 accggtcccg atggcaaacc cgtgctgctc gccaacatct ggccttccga caaggaagtc
      181 gccgacgcgg tgcacgccac gatcgactcg ggcatgttcc gcaagagtta cgccaacgtg
      241 ttcgcgggcg aagccaactg gcaggccatc gaaattccgc tgggcaagat ctacgactgg
      301 aaagaagaat cgacgtacgt acgcaatccg ccgtatttcg aaggcatgac catgacgccg
      361 cgcccggtca cggacgtcac cggcgcgcgc tgcctcgcct tgttaggcga ctcggtgacc
      421 accgaccaca tctcgcccgc cggcgacatc gccaagacca gcccggctgc gaaatacctg
      481 gtatccaagg gcgtgcagcc cctcgacttc aattcctacg gcgcgcgccg cggcaaccat
      541 gaggtcatga tgcgcggcac gttcgccaac atccgcctgc gcaacctgct cgccccgggc
      601 accgaaggcg gcgtcaccgt gcacgtgccg acgggcgagc agatgtccat cttcgatgcc
      661 gccatgcgct acaagcagga cagcacgccg ctcgtcattc tcgcgggcaa ggaatatggc
      721 accggctcgt cgcgcgactg ggccgccaag ggcaccatgt tgttaggcgt ccgcgccgtc
      781 atcgccgaga gcttcgagcg catccatcgc tccaacctca tcggcatggg cgtgttgccg
      841 ctcatgttcc cggtcggcaa gggtgcgttg tcactcggcc tcacgggcaa cgaggtgttc
      901 gagatcaccg gtctcgacaa cgccgaagcc aagaccgtgc aggtggtggc gactccgccg
      961 aagggttcgc ccatcaaatt cgaggcgcgc gtgcgcatcg acacgccgaa ggagcgcgac
     1021 tactaccgcc atggcggcat cctgcagtac gtgttgcggc agctggcctc caaggcagcc
     1081 tagttagcaa ggggacggtc cccaattcct gcctttgcaa aaggggctgg acctgaagca
     1141 ggtccggccc tttttgctag cctcttttgg aaaggattgg ggggactgtc cccattctta
     1201 aggcgctcgg cggccgcccg aacaggctga tgaacgcgcg ccgcatgcgc tcgggatcac
     1261 cgaagccgca gtcgcgcgcg atttcctgca cggaggcgcc gccgctttcg atgcgcgcgc
     1321 gggcggtttc ggcgcgaagt cgcatgacgg ccctggccgg gctggtgccc gtctcaccct
     1381 gaaacaggcg cgcgaaattc cgcgggctca tgcacgcctt tgccgcgagc tcatcgaccg
     1441 atagccgttc gcgcaggtgc gttcgcacat agtcgagcag cggcgcgaat tttccgtcag
     1501 cacgttcgag ttcgagcagc gtcgagaatt gcgactgtcc gcccggccgc cggtaataga
     1561 ccacgagctg ttgcgccacc ttgcgcgcca ccccttcgcc cacgtcttcg gcgatgagtg
     1621 cgagcgacag atcgatgccc gcggtgatgc cggccgagct ccagaatttc cccgccttca
     1681 cgaagatgcg atcggcatcg agcttcacgc gcggaaactt ctgcgcgaaa tcgttgctgc
     1741 gcgtccagtg cgtcgtggcg gcacgcccat cgagcaggcc ggtggcggcc agaatgtagg
     1801 cgcccgagca cacgctcgcg acgcgacgcg cctgtgcgcc gcaagcggcc acgaacttca
     1861 ccgtgcgtgc atccttcgat gcggcgcgcg taccttcccc gcctacgacg acgagcgtgt
     1921 ccaccgaggc cggcctcgat agcgacacgg cgttcatgct cgcgccggat gtgctcgtca
     1981 ccgcacccgg ctgcttcgcg atcacgcgca gctcgtaact tcccggcgcg tagcgctcgg
     2041 cgatttcgaa agcggaaatc ggccccgccg cgtcgagcag ctggaaatcg ggaaagagca
     2101 ggaaggcaac gcgtcgcttc atccgggcac cgttggcata aaacgaggga acaatggcct
     2161 ttccgccagc agggtatggg cccaccatca cgccgtcaat cgcgccatag cccaggagtt
     2221 caccgtgacc gccccattcc atgtcgtctt cgccctcttc ccgcgcatca ctcaactcga
     2281 tttcaccggt ccgtacgagg tgctcacccg cctgcctggc gcacgctgcg tgctggcatc
     2341 ggtgcaaggt ggcgacctcg aagtggatcc ggtcatgcgc ttctccggac tgcgccggct
     2401 cgacagtgtc gacgaatgcg atctgctttg cgtccccggc gggttcggga cggtggccgc
     2461 cgtcgaggac caggcctatc tagccgcgtt gcgccgcctc ggcgcgaagg cgcgctacat
     2521 cacctcggtg tgcaccggct cgttgttgct ggcggcggca ggacttctgc agggcaagcg
     2581 cgcggcctgc cactgggcgt ggcgcgacaa gctcgccgac ttcggcgtca cgcaggatcc
     2641 ggcgcgcgtg gtgcgcgacg ggatcgtctt caccggcggc ggcgtcaccg cgggaatcga
     2701 catggctctc gaggtcatgg ccgagatcgg gggccgcgac ctcgccgaga ccgtgcagct
     2761 cgccatcgag tacgcaccgg cgccgccgtt cgaatccgga cggccggagc tggcgcggga
     2821 gccggtcatt cgcgccgcgc gcgcccggct cgattccatg cgcgtcgagc gcgacgcggc
     2881 cattggacgg gccgccgccg cgctcaacgc agcctgagcc cgttcaactt tcctcgccca
     2941 attccttcgc gcgcatcctg ggcgtgacat tcgcccagtc gatgcgccgc gtggcgatca
     3001 tcagcgtggc caggactccg aacagcaaca gcgagcccat gagcaacgag tagtcctcgg
     3061 agcgcaggat ccagtacaac atggtgtaga gcatcgcgag gccggcgcct gcgcccacgg
     3121 ccagccagac gcgtcgcaat accccggtga gatagacggt gatcaacgcg accagcgctc
     3181 cggcggatac cgcatacgcg cgatcgaagg tcatgtgttc cgacaacgcc agcagcaata
     3241 gatagaacag ggccagcgcc aggccgacca tcaggtactg catgccgtgg atgggaatgc
     3301 cgctcacgtg ttcccagaga aagaacgtga ggaaggtgat gacgatcagc aggaccgcgt
     3361 agtgcacggc gcgatagttg cgctggtaga tgtcgacggg ctcgtaaaag gcgacgccca
     3421 ccctgctctg ggcgaacgac atctcggccg gttcgccggg ccgcacctgg tcgtcgtacc
     3481 agctctgccc gaaattgcgg ttgatctcca gcaccgacca gcgggcgctg aatcccttgt
     3541 cggtgatttc gctgtcgagc ggcgccggcg ctccctcgaa cttcggatgc ggccataggg
     3601 acttgatcga gatctccgcc ttgcgcgcca acggcaggaa attgagctgg ctggtgccca
     3661 ccagcgtgag tttcatgcgg aagggaatcg acgcgctttc gcgcaccgcc gccgcgggta
     3721 ccgccgcgga gatgccggcg gatcccgcat atccgtcggc cgacaccggc agccgctcgc
     3781 ccgccacctc gaggtcgtcc accgcgcgca gcgccctgga ttccgaattg agcaccagca
     3841 gccgcgcctc gccccatttc acttcgcggc cctgccgcgg ttgcagtagt tgggcgaagt
     3901 cgccattcac gaactctcct tcgacctgca cctgcgcctt gtagacgggc gtctggtaaa
     3961 gcccgacggt gcgatagtcc gtctcggcat tggcgctgac cttgagcgtg tccggcagaa
     4021 cgtaaagaac gttgcgcgag atttccgtcc gctgcgtttc acgtcccgcg gcggtctgct
     4081 cgacgaccag acgcgtggtg tccacgggta tcgccagcaa cacaccggcg gtcgtctgcg
     4141 cgccacccag cgactccgac actcgcatcg ccgcggtctg ccgcatgcca acgcgctctc
     4201 ccacgagatc ttccacctgg ccgagcggca ccagcagcag gacgatcagg aagccgatca
     4261 ccagtgcctt tgcaaccagg ggtggaatga atcggaatct ttcggccatc tcgttctccc
     4321 acacattgtt ttcgctgccg ggagtctcgg gaccaatgtg gccattggcg cggcgcaatt
     4381 gtggcgaatc gtggcaacgt cccgttggta acatgcggca tggccatcgc cttcttcgac
     4441 ctcgacggca cgatcactca tcgggacacc ctgtttccgc tggtgctgcg actgctggcg
     4501 tggccccggc cgtggcgctt gctgaaattg ctgcgcgtgg cgccagccgc cgttcgcttc
     4561 gcgttcgatc acgatcgggc cgctctcaaa caatccttgc tgcgcgccac cttgcgcggc
     4621 gcctcgcgcg acgaattgca caaggcctgc gaggtcttcg tgaccgacac gatcgcgcac
     4681 cgctgcttcg ccgacgcgct ggctgccgtg cgccggcatc gcgacgcagg ccactacctg
     4741 gtgctgatgt cggcgagcgt cgacttctac gtgcccgaat tcggccgaca gctcggcttc
     4801 gaccaggtca tctccaccgg cgtcgcctgg aacggcgacc gtctcgatgg aaccctgacg
     4861 acggccaacc ggcgtggcga cgaaaaagcc cgatgcctgc gcgcgctggt agatggacgc
     4921 cgcgacagcg aggtcttcgc ctacggcaac gccgcttcgg acctgccgca cctgcggctc
     4981 acgcgccacg gcctgttggt gaatggcagc gtggcggccc gacgcgaagc ggcgtcggtg
     5041 ggtgtggcgt gtgcagaatg gatctgatct gcacctaagg cccgccgggg tcgtgtgatc
     5101 tgcatcacag atatcaagag gtagcgctcc aacacggggc gggtaagcct tcgcatccga
     5161 cgccagtcac atagcccgtg agcacctcca actacgatga cggctggagt cgtccgtcgc
     5221 ttctggacaa tgtcaacaac acggggaaaa aaccggcact tcagattgat ggcgcgcgcc
     5281 gcatgcgccg cgctcgcctg tcttttttcg agtgccgcac tcgcctctcc gccgctcaac
     5341 gttcagcgct acagcctcga ggaaggcctg tcgcagcagt ccgtgaattc gatcgtccag
     5401 gacagcgaag gcttcatgtg gtttggcacc gaggacggac tcaaccggtt cgacggttat
     5461 gaatttcagc agttgcgcca cgaccgtggc gacaacgaaa cactgcccaa cggctggatc
     5521 tcttcgctgg tcgcgagcga tgaaggattg tggatcgcga ccgatggtgg cggcgtggtg
     5581 tttcgcagcg cgctcaccgg caaactcgac gcgccggcat tgttgcgcga tacgccggac
     5641 ctgcagcgcg tgcgcaccct ggcgcgagac cggctcggcc ggctgtggat cggtacgcgg
     5701 gactcgggca tcggcatctt cgacgtgcgc agcaaggagc ttcatcgcat ccggcattcg
     5761 ccttcgcaac cgaactcgct ctccgacaat tcgatcttca gcatcattca cctgcgcagt
     5821 ggcgacaccc ttatcggcac ggcgactggc ctcgatcgcc tgaccgcctc gaacctcgac
     5881 gtctcacaca tcgcgtggcc cgtcgaactg gttcccgcgg gccagccgtt gcgagtgcga
     5941 gccctcaccg aaacaccgga tggcatggtg tgggttggca cggatgcggg cctcggtcga
     6001 tacgatgcgc gtagtgcgcg ctggcgcgtc tatcgggaaa cacccgccga ctcgcccaat
     6061 cacttgagtt cgcttccgga caaccgcatc cagtcgctgc tcctcgacag ccagggccgt
     6121 ctttgggtgg gcatgctccg cggcctcgcg tggttcgatt cggcc
//
LOCUS       DATDEU010000222         4354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_476806, whole genome shotgun sequence.
ACCESSION   DATDEU010000222 DATDEU010000000
VERSION     DATDEU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476806"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1111
                     /locus_tag="VIV63_05485"
     CDS             122..1111
                     /locus_tag="VIV63_05485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6124080.1"
                     /translation="MGANKNDPGTMIPEASIVIGTLDRCDLLDRAIRSILDQGCDPAR
                     YEIVVVDNGSRDRTRLVVEKIMATAHNVRYVSEPRLGLSIARNRGIAESRAPIIGFFD
                     DDGTAEPGWLAKMLELFRQEPDIGAAGGLIKVAWPESQPEWMPMDLQGYYAGCDYGTT
                     RRYLDFPQYPYGPNMMIRRSLLDAIGGFNEHVGPKGQNIMSAGELDLFQRLYEQPIKV
                     VYEPTAVVHHWVPAERATRKWLLRRAYKHGFSSTRMKTVSKGRTRVAWFSLMTRALLR
                     SGMGCLAGTLGAATRAGKPVVTSRYAHMTYWGGVARGSLDGVLHGSALAPAPR"
     gene            1116..2123
                     /locus_tag="VIV63_05490"
     CDS             1116..2123
                     /locus_tag="VIV63_05490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6124081.1"
                     /translation="MSKEGWGMPEGEEKENEVTLEVSIVLCTLDRATQLTNAIRSILD
                     QDCDPRRYEIVVVDNCSKDGTPQVVQEFQSRASNVRYVYEGRLGLSTARNRGVAESRA
                     PIVAFFDDDGTADPGWLEALLEVFRSDGDAGAAGGLIHVGWPAEKPKWMPVDFQGYYG
                     ACDYGDERRSLAFPQYPYGANMMIRRQFLDAIGGFNDELGAKGQNIMQGDELDVFQRL
                     SDMKTKVVYEPRAVVHHWVPAERVTRKWLLRRAYKHGYSNTRLSSVRTGATRLTWTAL
                     LMRASFNCMVALLAVAAGATMARNVSVVTSRVAYVSYWAGVARGASDGLMHGLTKTAT
                     L"
     gene            2211..2834
                     /locus_tag="VIV63_05495"
     CDS             2211..2834
                     /locus_tag="VIV63_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY6124082.1"
                     /translation="MPLDTFLLYLATWTLVALSPGPAVMFSMSQAARHGMRGAAAGTA
                     GILLGHVVVFGAVALGLAALLASYSGAVTAIRIVGALYLMYLGAKMLFARARPANLPV
                     AAVAPPRHGGLALQGLVVQLTNPKLLLFVLALLPQFISPDYPLPLQLAIMLAVTVVID
                     GIALLAYAHLAARGARALKGSRALAWLERAFGGALIFFGLKLLVARK"
     gene            2960..3574
                     /locus_tag="VIV63_05500"
     CDS             2960..3574
                     /locus_tag="VIV63_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY6124083.1"
                     /translation="MTIRNVVFDVGGVLLEWNPPTVIARLYPDPATQAVIRSQIFEHA
                     DWLEFDRGTYSEDTAAEHFAKATGLTADQTRGLIRATRESLVPIAGTIKLVEELAGSG
                     VHLYLLSNMPVSTFDYLIQKHRFFNHFKHLVISGAILLVKPEPAIYKHLVDQTGILPG
                     ESVFIDDLLKNVIAARECGFHAIQFSDPASCREQLRAYLPGVSP"
     gene            3590..4249
                     /locus_tag="VIV63_05505"
     CDS             3590..4249
                     /locus_tag="VIV63_05505"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEY6124084.1"
                     /translation="MRSTRRIFLQQSAGIALAAHAGTAMAAEPAAPRAGGDLLSRMTW
                     LNPPAAEHYGQGVLTVRSKGKTDFWRKTFYGYVTDNGHFMHLPMLGEFTMQARINGNY
                     SALYDQAGLMVRLDEKHWMKCGSEFVEGKRWASVVVTHDFSDWSTMEDLSQTGAVWWR
                     VVRKKDSIEAQCSKDGEKFTTIRQGYFPADVKVMAGVMCAAPEGAGFDAVFDQLTIEK
                     A"
BASE COUNT          801 a         1389 c         1352 g          812 t
ORIGIN      
        1 gtccggccgg tcgcaccaag ttgcgacacg ccgcgcccga caattatgtc ggctgtcgga
       61 agtgttgaca gaccgctatt ttcccgctgt ctttcgcccg gctgcttgct agagtcgatg
      121 catgggagcg aacaagaatg accccggaac catgattccg gaggcgtcga tcgtcatcgg
      181 cacgctcgac cgttgtgact tgttagaccg ggcgatccgc agcatcctcg accagggttg
      241 cgatccggcc cgctacgaaa tcgtggtggt ggacaacggc tcccgggacc gcacgcgcct
      301 ggtcgtcgaa aagatcatgg ccacagctca caacgttcga tacgtgagcg aaccgcggct
      361 cggcctgtct atcgcgcgca atcgcggcat cgccgaatcg cgcgcgccca tcatcggatt
      421 cttcgacgac gacggcaccg ccgagcccgg ctggctcgcg aagatgctcg agctgttccg
      481 gcaggaaccg gacatcggcg ccgccggcgg gctcatcaag gtggcgtggc cggaaagcca
      541 gcccgaatgg atgccgatgg acctgcaggg ttattacgcc ggctgcgact acggcacgac
      601 gcgccgctat ctcgattttc cgcagtatcc ctacggtccc aacatgatga tccggcggtc
      661 gctgctcgac gcgatcggcg gattcaacga acacgtcggc ccgaagggcc agaacatcat
      721 gtccgccggc gaactcgacc tcttccagcg gctctacgag cagccgatca aggtggtcta
      781 cgagccgacc gcggtggtgc atcactgggt tcccgccgag cgtgcgaccc gcaaatggct
      841 gctgcgccgt gcctacaagc atggattcag cagtacccga atgaagaccg tcagcaaggg
      901 gcgaactcga gtcgcctggt tcagcctgat gacacgtgca ttgctgcgtt cgggaatggg
      961 ttgtcttgcc gggacattgg gcgcggccac gcgcgcggga aaacctgtcg tcacttcacg
     1021 ctacgcgcac atgacgtact ggggaggcgt cgctcgcggc tcgctcgatg gtgtactgca
     1081 cggatcagcg ctggcgcccg cgccgcgttg atgaagtgtc gaaagaaggg tggggaatgc
     1141 cggaagggga agaaaaggaa aacgaagtaa cgctcgaagt ctcgatagtt ctctgcacgc
     1201 tcgatcgcgc cacgcaactc accaacgcca ttcgcagcat cctcgaccag gattgcgatc
     1261 cgcggcggta cgagatcgta gtcgtcgaca actgctccaa ggatgggacg ccgcaggtcg
     1321 tgcaagaatt tcaaagccgc gcgtccaacg ttcgttatgt ttatgaaggc cggctcggcc
     1381 tgtccaccgc gcgcaatcgt ggtgtggcgg agtcgcgcgc accgatcgtg gcgttcttcg
     1441 atgatgacgg caccgccgat ccaggctggc tggaagcgct gctcgaggtc tttcgcagtg
     1501 acggcgatgc cggcgctgcc ggcggcctca tccacgttgg ctggcccgcc gaaaagccca
     1561 agtggatgcc cgtcgatttt caggggtact acggcgcctg cgactatggc gacgagcgcc
     1621 ggagtctcgc gttcccgcaa tacccgtatg gcgcgaacat gatgatccgc cgccagttcc
     1681 tcgacgccat cgggggcttc aacgacgaac tcggcgcgaa gggccagaac atcatgcagg
     1741 gcgacgagct cgatgtgttc cagcgcctgt ccgacatgaa aaccaaggtg gtctacgaac
     1801 cgcgcgcggt cgtgcaccac tgggttccgg ccgaacgtgt cacgcgcaag tggctgctac
     1861 gacgcgcgta caaacatggc tacagcaata cgcgcctctc atcggtgcgc acgggagcga
     1921 cgcgcctcac gtggaccgcg ctgttgatgc gggcatcgtt caattgcatg gtggccctgc
     1981 tggcagtggc cgccggcgcg accatggcga gaaacgtttc ggtggtcacc tcgcgggtcg
     2041 cgtacgtgag ttattgggcg ggcgtcgcgc gcggcgccag cgacggtctc atgcacggcc
     2101 tcaccaaaac cgcgacgctc tgatcccgaa gccgcgtccg atacagcgac cgcgggaacc
     2161 gcgcgaactg tcacgggact tggtctctgg ctcccgctac tgtattcggg atgccactcg
     2221 atacttttct gctgtatctg gcgacctgga cgctggtcgc tctttcgccc ggtcccgcgg
     2281 tgatgttctc catgtcgcag gcggcgcgtc acggcatgcg tggcgcagcg gcgggcaccg
     2341 ccggaattct cttgggtcac gtggtcgtct tcggcgccgt tgcgctggga ctcgcggcgc
     2401 tgctggcaag ttatagcggc gcagtcaccg ccatccgcat cgtcggcgcg ctgtacctga
     2461 tgtatctcgg cgcaaaaatg ctgtttgccc gggcgcgccc cgcgaatctg cccgtcgcgg
     2521 cagtcgcccc gcctcgtcac ggcggcctcg cgctgcaggg actcgtcgtg cagctcacga
     2581 atcccaagct gctgctgttc gtgctcgcgt tgctgccgca gttcatcagt cctgactacc
     2641 cgctgccgct gcagctcgcc atcatgctgg ccgtcacggt cgtgatcgac ggcattgcac
     2701 tgctggccta cgcacacctc gcggcccgcg gcgcgcgcgc actcaagggc tcgcgtgcgt
     2761 tggcgtggct ggagcgcgcc ttcgggggcg cactgatttt cttcggattg aagctgctcg
     2821 tcgcccgcaa gtaggcggaa aaaaggggac ggaaaaaagg ggacagatct attttttgcg
     2881 gatacctgcg tatccgcaaa aaatagatct gtcccctttt ttccgtcccc ttttttcgcc
     2941 ctgcttgcat actacgggca tgaccataag gaacgttgta ttcgatgtgg gcggtgtgtt
     3001 gctcgagtgg aacccgccca ctgtcatcgc ccgcctctat ccagatcccg cgacccaggc
     3061 ggtcatccgc agccagatct tcgaacatgc cgactggctc gaattcgatc gtggcaccta
     3121 tagcgaagac acggccgccg aacatttcgc caaggccacc gggctcacgg ccgaccagac
     3181 gcgcggactc atccgcgcca cgcgcgagtc gctggtcccc atcgcaggca cgataaagct
     3241 ggtcgaagaa ctcgcaggtt caggcgtgca tctctacctg ctgtcgaaca tgccggtgag
     3301 cacgttcgac tacctcatcc agaaacaccg gttcttcaat cacttcaaac acctggtgat
     3361 ctccggcgcc atcctgctgg tgaaacccga acccgccatc tacaaacacc tggtcgacca
     3421 gaccggtatc ctgcccggcg agagtgtgtt catcgacgac ctgctcaaga acgtgatcgc
     3481 cgcgcgcgag tgcggcttcc acgccatcca gttcagcgac ccggcgagct gccgcgagca
     3541 gctccgcgcc tatctacccg gcgtctcacc ctgacggagg actctgttca tgcgctccac
     3601 gcgaaggatc tttctgcagc agtcggccgg catcgcgctg gccgcgcacg ccgggacggc
     3661 catggccgcc gagccagccg cgccgcgtgc cggcggcgat ctcctgagcc gcatgacctg
     3721 gctcaatccg ccggccgccg aacactacgg ccagggcgtg ctcacggtgc gctccaaggg
     3781 caagaccgat ttctggcgca agaccttcta cggctacgtc accgacaacg gccatttcat
     3841 gcatctgccg atgctcggcg agttcacgat gcaggcacgc atcaacggca actattcggc
     3901 gctctatgac caggccgggc tcatggtccg actcgacgag aagcactgga tgaagtgcgg
     3961 ttccgaattc gtcgaaggca agcgctgggc gagcgtggtg gtcacgcacg atttctccga
     4021 ctggtcgacc atggaagatc tgtcgcagac cggcgcggtg tggtggcgcg tggtgcgcaa
     4081 gaaagattcc atcgaagcgc agtgctccaa agacggtgaa aaattcacga cgattcgcca
     4141 gggctacttt ccggccgacg tgaaagtgat ggccggcgtg atgtgcgctg caccggaggg
     4201 tgcgggtttc gatgcggttt ttgaccaatt aacgatcgaa aaagcttaag ttcgatcacg
     4261 gtggttgggc gtatgtgagg cattccacct ttccccgccc cgggcactac agatgaaggc
     4321 agaggggccg ttaaacggga gtcgtgaaca gcct
//
LOCUS       DATDEU010000223         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1060457, whole genome shotgun sequence.
ACCESSION   DATDEU010000223 DATDEU010000000
VERSION     DATDEU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060457"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VIV63_05510"
     CDS             complement(<1..261)
                     /locus_tag="VIV63_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124085.1"
                     /translation="MATATADTKLGKVIQVIGPVIDVEFESGYLPNIYNALRIVATLP
                     TGEHIDIITEVEQHLGEGRVRTVAMKPTDGLQRGMDVQDTGAP"
     gene            complement(270..1181)
                     /gene="atpG"
                     /locus_tag="VIV63_05515"
     CDS             complement(270..1181)
                     /gene="atpG"
                     /locus_tag="VIV63_05515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY6124086.1"
                     /translation="MPSLIDIRRRVKAVKSTQQITKAMKMVSTSKLRRAQERIVKSRP
                     YAKEMLRVFNHLATRTENSAHPLLNDDPEAGRTLLIVITADRGLAGSFNTNVAKAALQ
                     FTIDQPKDPQGRDVAMALIGRKGRDFFMRRGFDVLYEETGIFQNIKWSHAQAIAQTAI
                     KEFLGPDISSVYLVYNEFRSVMSQRVVIEKLLPIPRLNEAEAANEGKPAASADAFGSP
                     SVDYLFEPEPEVLLNMLLPMHVAVQVQRALLESSAAEHAARMQSMDSATRNAKEMVER
                     LTLYMNKVRQAAITREIIEVVSGAQAT"
     gene            complement(1186..>2049)
                     /locus_tag="VIV63_05520"
     CDS             complement(1186..>2049)
                     /locus_tag="VIV63_05520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY6124087.1"
                     /translation="VAATASDPAPMLYISPYSATAMGEYFRDSGRHALLIYDDLSKHA
                     QSYREISLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDKLGGGSLTSLPIIETQAGD
                     LSAYIPTNVISITDGQIFLEADLFNQGVRPAINVGNSVSRVGGSAQIKAMRQVAGTLR
                     LDLAQFRELSAFAQFGSSDLDPATQRQLNRGARLVEVLKQKQYAPLSVDQQVTILYAG
                     VNGFLDEIAVKDVGAAQDELVKFMTTRHNGVLSAIKEKKNLDDALKADLNAALTEFQA
                     HFKSMAGMAKA"
BASE COUNT          337 a          655 c          646 g          411 t
ORIGIN      
        1 cggcgcgccg gtgtcctgca cgtccatgcc gcgctgcaga ccgtcggtcg gcttcatcgc
       61 gaccgtgcgg acgcgacctt cgccgaggtg ctgttcgacc tcggtgatga tgtcgatgtg
      121 ctcgccggtg ggcagcgtcg ccacgatgcg aagggcgttg tagatgttgg gaaggtagcc
      181 gctctcgaac tcgacgtcga tgacgggtcc gatgacctgg atgaccttgc cgagtttcgt
      241 atctgccgtt gctgtagcca tatttgtctc tacgtagctt gcgcgcccga aacgacttcg
      301 ataatttccc gcgtgatagc cgcctgtctc accttgttca tgtagagggt gaggcgctcg
      361 accatttctt tcgcgttgcg tgtcgcgctg tccatcgact gcatgcgcgc cgcatgttcc
      421 gccgccgatg attcgagcaa cgcgcgctgg acctgcaccg ccacgtgcat cggcagcagc
      481 atgttcaaca gcacctccgg ctcgggctca aacaggtaat ccaccgaggg cgacccaaat
      541 gcgtccgcgc tcgcggcagg cttgccttca ttcgccgcct ccgcctcgtt gaggcgggga
      601 atcggcagca gcttttcaat caccacgcgc tgcgacatca ccgagcggaa ctcgttgtag
      661 accaggtaga ccgaactgat gtcgggtccg aggaactcct tgatcgcggt ctgcgcgatc
      721 gcctgggcgt gcgaccactt gatgttctgg aagatgccgg tctcttcgta gaggacgtcg
      781 aaaccgcggc gcatgaagaa gtcgcgtccc ttgcggccga tcagcgccat cgccacgtca
      841 cgcccctgcg gatccttggg ctgatcgatc gtgaactgca gcgcggcctt ggcgacgttg
      901 gtgttgaagc tgccggccag accacggtcg gcggtaatca cgatcagcag cgtgcggccg
      961 gcttccggat cgtcgttcaa cagcggatgc gcgctgttct cggtgcgcgt cgccaggtgg
     1021 ttgaacaccc gcagcatctc cttcgcgtac gggcggctct tgacgatgcg ctcctgcgcg
     1081 cggcgcagct tcgacgtcga gaccatcttc atggccttcg tgatctgctg cgtcgacttg
     1141 accgctttga cgcggcgccg gatgtctatg agtgacggca tgctgctacg ccttcgccat
     1201 ccccgccatc gacttgaagt gcgcctggaa ttccgtcaac gccgcattga ggtcagcctt
     1261 caacgcatcg tcgaggttct tcttttcctt gatcgccgac agcacgccgt tatgacgcgt
     1321 ggtcatgaac ttgacgagct cgtcctgcgc ggcgccgacg tccttgaccg caatttcatc
     1381 gaggaatccg ttgacgccgg cgtacaggat ggtgacctgc tgatcgaccg acagcggcgc
     1441 gtactgcttc tgcttcagca cttcaacgag gcgcgcgccg cgattcagct ggcgctgcgt
     1501 cgccggatcg aggtcgctgc tgccgaactg cgcgaacgcg gacagctcgc ggaactgcgc
     1561 aaggtcgagc cgcaacgtgc cggccacctg ccgcatcgcc ttgatctgcg ccgagccgcc
     1621 gacgcgcgac accgagttgc cgacgttgat cgccgggcgg acgccctggt tgaagaggtc
     1681 cgcttccagg aagatctgcc cgtcggtgat cgagatgaca ttggtcggaa tgtacgccga
     1741 caagtcgccc gcctgcgttt cgatgatcgg cagcgaggta agcgaaccgc cgcctagctt
     1801 gtcgttcagc tttgccgccc tctccagcag acgcgaatgc aggtagaaga cgtcgccggg
     1861 ataggcctcg cggccgggcg gacggcgcag cagcaacgag atttcacgat acgactgcgc
     1921 gtgcttcgag aggtcgtcgt aaatcagcag cgcgtgccgg cccgagtcgc ggaagtactc
     1981 gcccatcgcg gtcgccgagt acgggctgat gtagagcatc ggcgccggat ccgacgcggt
     2041 cgcggccac
//
LOCUS       DATDEU010000224         5283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1934851, whole genome shotgun sequence.
ACCESSION   DATDEU010000224 DATDEU010000000
VERSION     DATDEU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934851"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VIV63_05525"
     CDS             <1..385
                     /locus_tag="VIV63_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY6124088.1"
                     /translation="QRNLAVNDFARRDHEYVRADCIAWLRDAVAERRSYDLIFLDPPT
                     FSNSKRMEDILDVQRDHASLIDSCMALLAPGGKLVFSTNAQKFKLDGMLGERYKVTDI
                     SRATLPKDFERNPRIHQCYELESGK"
     gene            382..804
                     /locus_tag="VIV63_05530"
     CDS             382..804
                     /locus_tag="VIV63_05530"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008008030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY6124089.1"
                     /translation="MIKKGSAAWKGSLKEGTGSISTETGVLKNAPYGFKARFEDGPGT
                     NPEELIAAAHAACYSMALSAGLGKEGFTADSIETSAAVRLEKIGDGFAITHSDLICLA
                     KVPNISEQKFAEIAEITKSGCPVSKVLNAKISLVAKLI"
     gene            963..3002
                     /locus_tag="VIV63_05535"
     CDS             963..3002
                     /locus_tag="VIV63_05535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY6124090.1"
                     /translation="MKATFCAGVGAAILFASAAGAAALPEAQRLHSIDVFQLEYTDDV
                     QISPDGNRIVYVRISHDIMTDRARRNLWIVNADGTNNRPLRSESKNFSSPRWSPDGTR
                     LAYVSAAEGSPQLYVRWMDSGQTALLTNLVESPGAIAWSPDGKSIAFTQLVPAKQTPL
                     ATPPEKPEGAQWAPPVRVVDTVTYRADGAGWLEAGYQHVFLVSSDGGTARQLTAGDFN
                     HEGPLSFTPDSKNIVFSANRDADWERNPQASNVFAVNTDTAKLTQLTTREGPDNSPVV
                     SPDGQKIAYLGFDDRLQGYQVTHLYVMDVDGKNPRLVTGEFDLDVDATLWSSDSRSLY
                     FSFDERGVKKLGVVSLDGKVRTLAVGLGGADLGRPYTSGSFSVSKNGRAAFTHNTTGR
                     PADVALYSGGSQRVLTSLNDDLLGHKKLGDVREVNWKSSKDQRDIQGWVITPPDFDKS
                     KKYPLILEIHGGPFTAYGPNYTTELQLFAAAGYIVLYANPRGSTSYGETFGNLIHHAY
                     PGDDYDDLMSGVDAVIAEGNVDTNNLFVTGGSGGGVLTAWIVGKTDRFRAAVVAKPVI
                     NWASFALTADMSNFFYKYWFGAQPWEQPEQYWKRSPLSLVGNVKTPTMLLTGESDYRT
                     PISESEQYYQALKLRKVDTVMVRIPEASHGMTARPSNLIAKVDNILAWFEKYRKK"
     gene            3017..3967
                     /locus_tag="VIV63_05540"
     CDS             3017..3967
                     /locus_tag="VIV63_05540"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY6124091.1"
                     /translation="MRFAILGAGALGTILGAHLSRAGHDVVMIARGERARVLQRQGLV
                     LNGLSDTKARPNVIDDPSKLIDTDTLIVATKAIDSARSLDTLKHLRLENAFSVQNGVL
                     KNELLARVFGYSRVLGAMADFSGELLANGEVKFTRNVCLHLGEEKGGSSARAENMAAL
                     IDAAGVRCVSASNIRTREWSKFTGWIAQFPLAVLTRQITWKFLMDESSAMVIVRIVRE
                     AAALAAAMKIELTDMPPLPVPSIVHGTDAQAIEIVRGIGQKFLDTSPEHRMSAQQDVL
                     RGSRLEYEETLGYALAKARELNVPMPTLDVCYRIIAVAHP"
     gene            4120..4824
                     /locus_tag="VIV63_05545"
     CDS             4120..4824
                     /locus_tag="VIV63_05545"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124092.1"
                     /translation="MPGAPWSEATFKSGAVITVDAQHAFSGNQAMHIFTPRGAKYRRG
                     YVAIHLKTPVPDAMPGMYGRAMVYLDEAPVALPGAPPVHWTLLQGEGRSADDRYNSIY
                     RLGVEGRGGTQLMANFETTPPVTTDCKQNSTRTLPVKRWTCVEWHMEVATNEMQFWLD
                     GRKVVHVAGKTAAANACRGNDLQGEWRAPPKFDSFYIGFERYADSANDQNLWIDDVAL
                     SKTRVGCSVPKRGEDR"
     gene            4821..>5283
                     /locus_tag="VIV63_05550"
     CDS             4821..>5283
                     /locus_tag="VIV63_05550"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6124093.1"
                     /translation="MKYFSLAALFLVLGSAVAADAAKPVPAPVPAAPVPPPPALVDPA
                     AIDSALKSMIDSRKVIGVSALVYERGQEAYFGAFGLADRENNKPMARDTVVQIFSMTK
                     PVTGVALMQLYERGKFELDAPLSVYLPEFADVRVYAGLDANGQPKYEPPKRP"
BASE COUNT         1018 a         1752 c         1628 g          885 t
ORIGIN      
        1 tcagcgcaat ctggcggtga atgacttcgc gcggcgcgat cacgaatacg tccgcgccga
       61 ctgcatcgcc tggctcaggg acgcggtggc ggaacgccgc tcctacgacc tgatttttct
      121 cgatccgccc acgttttcca acagcaaacg catggaggac atcctcgatg tgcagcgcga
      181 tcacgcctcg ctgatcgata gctgcatggc gttgctggcg ccgggcggca agctggtgtt
      241 ctcgaccaac gcgcagaagt tcaagctgga cggcatgctc ggtgagcgtt acaaagtcac
      301 cgacatttcg cgcgccacgc tgccgaaaga cttcgagcgg aatccgcgca ttcatcagtg
      361 ttacgaactg gagtcaggaa aatgatcaag aaaggttccg cagcctggaa gggcagtctc
      421 aaggaaggca cgggcagcat ctccaccgaa accggcgtgc tcaagaatgc gccctacggt
      481 ttcaaggcgc gcttcgaaga tggtccgggc accaaccccg aagagctcat cgccgccgcg
      541 cacgccgcgt gttattcgat ggcgctgtcg gcgggcctcg gcaaggaagg gttcaccgcg
      601 gactccatcg agaccagcgc ggcggtgcgg ctggaaaaaa tcggggacgg ctttgccatc
      661 acgcacagcg atctcatctg cctcgccaag gtgcctaaca tcagcgagca gaaattcgcg
      721 gagattgccg agatcaccaa gtccggttgc ccggtctcga aagtgctgaa cgccaagatc
      781 tcgctggtgg cgaagctgat ctagcgcagg tttcaggacg ccggtggcag aaggccggcg
      841 tcctcctccg aaagtcgaat agcccggccg gggcgcgagc ggctggcaaa aagtacacgc
      901 gccccgccgc agggcgtggt ccctcggtta aagtagccgc actttcctat tcgggacgct
      961 ccatgaaagc cactttttgc gccggggtcg gcgcggccat tttgtttgct tccgcggcgg
     1021 gcgctgccgc cctgcccgaa gcgcagcgcc tgcactccat cgatgtgttc cagctcgaat
     1081 acaccgatga cgtgcagatt tctcccgatg gcaatcgcat cgtctacgtg cgcatcagcc
     1141 acgacatcat gaccgaccgc gcgcggcgca atctgtggat cgtcaacgcg gacggcacta
     1201 acaatcggcc gctgcgttcc gaatcgaaaa atttcagctc gccgcgctgg tcgccggacg
     1261 gcacgcgcct cgcgtatgtg tccgcggccg aaggcagtcc gcagctctat gtacgctgga
     1321 tggattccgg gcagacggcg ctcctcacca atctcgtcga gtcgccgggc gccatcgcct
     1381 ggtcgccgga cggaaagtcg atcgcattca cccagctcgt gccggcgaag cagactccgc
     1441 tggccacccc accggaaaag cccgaaggcg cgcaatgggc gccgccagtc agggtcgtcg
     1501 acaccgtcac gtatcgcgcc gacggcgcgg gttggctcga agccggctac caacacgtgt
     1561 tcctcgtctc gtcggatggc ggtacggcgc gccagctcac cgcgggcgac ttcaaccacg
     1621 aaggtccgct gagcttcacg cccgacagca agaacatcgt gttctccgcc aatcgtgacg
     1681 ccgactggga acgcaatccg caggcgagca acgtgttcgc cgtgaacacc gacaccgcga
     1741 aactcacgca gctcaccacg cgtgagggac cggacaactc accggtggtg tcgcctgacg
     1801 gccagaaaat cgcctacctg ggtttcgacg accgcctgca gggttaccag gtcactcatc
     1861 tatatgtgat ggatgtcgac gggaaaaacc cgcgactcgt gaccggcgaa ttcgatctcg
     1921 acgtggatgc cacgctgtgg tcgtccgaca gccgcagcct gtatttctct ttcgacgaac
     1981 gcggcgtgaa aaagctcggc gtcgtctcgc tcgatggcaa ggtgcgcacg ctggctgtgg
     2041 gcctgggtgg cgccgacctc ggccggccct acacctcggg ctcattcagc gtctcgaaga
     2101 acggccgcgc ggcattcacg cacaacacca ccggccgccc ggccgatgtc gcgttgtact
     2161 ccggcggttc gcagcgcgtg ctgacctcgc tcaacgacga cctcctgggc cacaagaaac
     2221 tgggcgacgt tcgcgaggtc aactggaagt cgtcgaagga tcagcgcgac atccagggct
     2281 gggtcatcac gccgcccgat ttcgacaagt cgaagaaata tccgctcatc ctcgagatcc
     2341 acggcgggcc gttcaccgcc tacggtccta actacaccac cgagctgcag ctgttcgccg
     2401 cggccggcta catcgtgctg tacgccaacc cgcgtggcag caccagctac ggcgaaactt
     2461 tcggcaacct gatccaccac gcctatcccg gcgacgacta cgacgacctc atgtccggcg
     2521 tggatgcggt catcgcggaa ggcaatgtcg ataccaacaa cctgttcgtc accggcggct
     2581 ccggcggcgg cgtgctcacg gcgtggatcg tcggcaagac cgaccggttc cgcgcggcgg
     2641 tggtcgccaa gccggtcatc aactgggcca gcttcgcgct cacggcggac atgagcaatt
     2701 tcttctacaa atactggttc ggcgcgcagc cctgggagca gcccgagcag tactggaagc
     2761 gttcgccgct atcgctggtg ggcaacgtga agacgccgac catgctgctc acgggcgagt
     2821 ctgactaccg cacgccgatc tcggaatcgg agcagtacta ccaggcgctg aagctgcgca
     2881 aggtggacac ggtgatggtg cgtattcccg aagcgtcgca tggcatgacc gcgcggccca
     2941 gcaatctcat cgccaaggtg gataacatcc tcgcctggtt cgaaaagtac cggaaaaagt
     3001 aggagcaagg aggctcatgc gtttcgcaat cctcggcgcc ggcgcccttg gcaccatcct
     3061 gggcgcgcac ctgtcgcgcg ccggacatga cgtcgtgatg atcgcgcgcg gtgagcgcgc
     3121 ccgagtcctg cagcgccagg gtctggtgct caacggactt tccgacacca aggcgcggcc
     3181 caacgtcatc gacgatccgt cgaagctcat cgacaccgac acgctcatcg tggccaccaa
     3241 ggcgatcgac tccgcgcgca gcctcgatac gctcaagcac ctgcggctcg agaacgcgtt
     3301 ttcagtgcag aatggcgtgc tcaagaacga actgctcgcg cgggtcttcg gctattcacg
     3361 cgtgctcggc gccatggcgg atttcagcgg cgaactgctg gccaatggcg aagtgaagtt
     3421 cacgcgcaat gtctgcctgc acctcggcga ggagaagggc ggcagcagcg cacgcgcgga
     3481 gaacatggcc gcgctcatag acgccgccgg cgtgcgctgc gtgtcggcgt ccaacatccg
     3541 gacgcgcgag tggtcgaagt tcacgggctg gatcgcgcag ttcccgctgg ccgtgctcac
     3601 gcggcagatt acctggaaat tcctcatgga cgagagcagc gcgatggtca tcgtgcgtat
     3661 cgtgcgtgaa gccgcggcgc tcgccgcggc catgaagatc gagctgaccg acatgccgcc
     3721 gctgcccgtg ccgagcatcg tgcacggcac ggacgcccag gccatcgaaa tcgtgcgcgg
     3781 aatcggccag aagtttctcg acacctcgcc cgaacaccgc atgtccgcgc agcaggatgt
     3841 cttgcgcggc agccggctcg aatacgagga gacgctcggc tacgcgctgg ccaaggcgcg
     3901 cgaactcaac gtgcccatgc ccaccctcga cgtctgctat cgcatcatcg ccgtggccca
     3961 cccgtgatgg gcgctgcgaa atggggacat tcccgatttg ctagcaaatg gggacgcgcc
     4021 tcgttttccg tggccgcgat tttcgccgct agcgccgcgc tcgctgagcc accgcgcatc
     4081 ctgttcaacg ataatttcga agcgcacgca acaggcgcaa tgcccggcgc cccctggagc
     4141 gaagccacct tcaaatccgg cgccgtcatc accgtcgacg cccaacacgc gttcagcggc
     4201 aaccaggcga tgcacatctt cacaccccgc ggcgcgaaat accgtcgcgg ctatgtcgcg
     4261 atccacctga aaaccccggt gcccgacgcc atgcccggca tgtacggccg cgccatggtc
     4321 tatctcgacg aggcgccagt ggcgcttccc ggcgctccgc ccgtgcactg gacgctgctg
     4381 caaggcgaag gccgctccgc cgacgatcga tacaactcta tctaccggct cggcgtcgaa
     4441 gggcggggcg gtacgcagct catggccaac ttcgaaacca cgccgccggt caccaccgac
     4501 tgcaagcaga actcgacgcg gaccctgccg gtgaagcgtt ggacctgcgt cgaatggcat
     4561 atggaagtcg ccacgaatga aatgcagttc tggctcgatg ggcgcaaagt cgtccacgtc
     4621 gccggcaaga ccgcggcggc caacgcctgt cgcggcaacg acctgcaggg cgagtggcgc
     4681 gcaccaccga agttcgacag cttctacatc ggcttcgagc ggtacgcgga ttccgccaac
     4741 gatcagaacc tgtggataga cgacgtcgcg ctgtcgaaga cacgcgtcgg atgttccgtg
     4801 ccgaaacgag gagaggatcg atgaagtatt tcagtctggc cgcattgttt ctggtgctgg
     4861 gttcggcggt tgccgcggac gcggccaaac ccgtgccggc tcccgtccca gctgctcccg
     4921 tgccgccgcc gcccgcgctc gtggacccgg cggccatcga cagcgcgctc aagagcatga
     4981 tcgacagccg gaaggtcatc ggcgtttccg cgctggttta cgagcgcggc caggaagcct
     5041 acttcggtgc cttcggactg gcggatcgcg agaacaacaa gccgatggcg cgcgataccg
     5101 tcgtgcagat cttttcgatg acgaagcccg tcaccggcgt cgcgctcatg caactctacg
     5161 agcgcggaaa attcgaactc gatgcgccgc tctcggtgta tctgccggag ttcgccgacg
     5221 tgcgggttta tgcgggcctc gatgccaacg gccagccgaa gtacgagccg cccaagcggc
     5281 cca
//
LOCUS       DATDEU010000225         3789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1061009, whole genome shotgun sequence.
ACCESSION   DATDEU010000225 DATDEU010000000
VERSION     DATDEU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061009"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VIV63_05555"
     CDS             <1..284
                     /locus_tag="VIV63_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEY6124094.1"
                     /translation="FVNAHRIPAIYQAAMFADAGGLMAWSPDQVEQQRMAARLAAKIL
                     KGAKPGDLPVTYPTGYGLTINADTARKTGITLPPALLAQAARVIPASGS"
     gene            complement(281..1048)
                     /locus_tag="VIV63_05560"
     CDS             complement(281..1048)
                     /locus_tag="VIV63_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY6124095.1"
                     /translation="MRTTDHRATRLFIFLAGFFLTNALIAEFVGVKIFSLEDSVGAAP
                     FHWSLFGINGTLNFTAGVLLWPFVFIMTDVINEYFGVRGVRLISWIAVGFIAYAFVAA
                     YAAISLAPATFWVDVNAGLGVPDIQKSYALIFGQGLWTIGGSIIAFLVGQLIDVAIFH
                     RIQRITGERFVWLRATASTAVSQLLDSFIVLYIAFVIGPQHWSIPQFLAVGTVNYAYK
                     MTAAIALIPLLYLARAGIRAYLGQGEAERLRREAAGD"
     gene            complement(1062..1496)
                     /locus_tag="VIV63_05565"
     CDS             complement(1062..1496)
                     /locus_tag="VIV63_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY6124096.1"
                     /translation="MRPKPTDHEDVEGAEGGGTSLRIDRWLWCARMFKSRTLAADAVA
                     GGKVHVNGQRVKPAHSVRVGDRLSLKRPGYEFECEVLKLPDRRGSAPVAQACYSETDA
                     ARAAREKFAEQSRLVSAFAQPRSLDRPDKHGRRELRRLRGRD"
     gene            1544..2731
                     /locus_tag="VIV63_05570"
     CDS             1544..2731
                     /locus_tag="VIV63_05570"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY6124097.1"
                     /translation="MDPAFYQRFDTQLDELRQQGLFKTERVIASPQAGQIRANDADVI
                     NLCANNYLGLASHPAIREAAHRAIDRYGYGMASVRFICGTQTVHKQLEAALSRFLGTE
                     DTILYSSCWDANGGLFETVLGEQDAIISDGLNHASIIDGIRLCKARRLRYANRNLAEL
                     EQHLKDTQDCRTRLIATDGVFSMDGSIAPLREICDLADKYGALVMVDDSHATGFMGAG
                     GRGTPEHCGVASRIDFLTGTFGKALGGGSGGYTSGKANAIAWLRQRSRPYLFSNSIPP
                     VVAAVTLRALDLVENMPDLRATLFGHARKFRAGMQAAGFKLLPGEHPIIPVMIGDAAL
                     AGQFADRLLGEGVYVIGFSFPVVPKGEARIRTQMSAGLNDAQLERALAAFIKVGRELE
                     LIK"
     gene            2728..3762
                     /gene="tdh"
                     /locus_tag="VIV63_05575"
     CDS             2728..3762
                     /gene="tdh"
                     /locus_tag="VIV63_05575"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY6124098.1"
                     /translation="MKALVKSEAKPGIWMKDIPEPTVGPNDVLIKIAKTAICGTDMHI
                     YHWDAWAQKTIPVPMAVGHEYFGRIVDMGSEVRGFAIGDRVSGEGHLTCGFCRNCRAG
                     RRHLCRNTVGVGVDRQGCFAELLVIPASNAFKLPDAVTDDIASILDPFGNATHTALAF
                     NMVGEDVLITGAGPIGIMAVAISKKIGARHVVITDVNDYRLGLAQKMGATRAINVTKQ
                     TLDDTMNELGMQEGFDVGLEMSGNPAAFRDMLRTMHHGGSVALLGIPPGETAIDWNQV
                     IFKGLNMRGIYGREMFETWYKMAALLQAGLDITPVITHHFPIQEFEQGFKTMGSGQSG
                     KVILDWGTLS"
BASE COUNT          731 a         1265 c         1182 g          611 t
ORIGIN      
        1 acttcgtcaa cgcgcatcgc atccctgcca tctaccaggc ggccatgttc gcggacgcag
       61 gcgggctcat ggcctggtca ccggatcagg tcgagcagca gcgcatggcc gcgaggctcg
      121 cggcgaaaat cctcaagggt gcgaagccgg gcgacctgcc cgtgacctat cccaccgggt
      181 acggtctcac catcaatgcc gatacggccc ggaaaaccgg cattacgttg ccacccgcat
      241 tgctggcgca ggccgcgcga gtgatccccg cgtccggtag ctagtcgccg gccgcttcgc
      301 ggcgcaggcg ctccgcttca ccctgcccga ggtacgcacg aatgcccgcg cgcgcgagat
      361 acagcaaggg aatcagcgca atggcggccg tcatcttgta tgcgtaattc accgttccca
      421 ccgcgaggaa ctgcggaatg gaccagtgct gcggcccgat gacgaaggcg atgtacagca
      481 cgatgaaact gtcgagcagc tgcgagacgg cggtggacgc ggtggcgcgc agccacacga
      541 aacgctctcc cgtgatgcgc tggatgcggt ggaaaatcgc gacgtcgatg agctgcccga
      601 cgagaaacgc gatgatggat ccgccgatcg tccacagtcc ctggccgaag atgagcgcgt
      661 aggatttctg gatatcgggc acgccgagcc cggcgttcac gtcgacccag aacgtggcgg
      721 gcgccagcga aatggccgca taggcagcca cgaatgcata ggcgatgaac ccgaccgcga
      781 tccacgagat gaggcgcacg ccccgcacgc cgaagtattc gttgatgacg tcggtcatga
      841 tgaacacgaa cggccacaac aacacgccgg cggtgaagtt cagggttcca ttgatgccga
      901 acagcgacca gtgaaacggc gctgcaccga cgctgtcttc cagcgaaaag attttcacgc
      961 cgacgaactc ggcgatcagc gcattcgtga ggaagaatcc cgccaggaag ataaacagac
     1021 gggtcgcgcg gtgatcggtg gtgcgcatgc gtggctccgc gtcagtcgcg gccgcgaagg
     1081 cggcgcagtt cgcggcggcc gtgtttgtcg gggcggtcca gcgaacgtgg ttgggcgaat
     1141 gccgacacca ggcgagactg ctcggcgaat ttctcccgcg ccgcgcgcgc ggcatcggtc
     1201 tcggaataac acgcctgcgc gacgggcgcc gaaccgcggc ggtcaggcag tttcaggact
     1261 tcgcattcga attcataacc gggtcgtttg agcgaaagcc ggtcgccgac ccgcacggaa
     1321 tgcgccggtt tgacgcgttg gccgttcaca tggaccttgc cgcccgccac ggcgtcggcc
     1381 gccagcgtgc gcgacttgaa catccgggcg caccacaacc agcggtcgat gcgcagcgag
     1441 gtgccaccgc cttcggcgcc ctccacgtcc tcgtgatctg tcggcttggg tctcacggcg
     1501 gccagtataa ttgcgccaac ttcaccttac ccgaggttcc ttcgtggacc ctgcgttcta
     1561 ccagcgattc gacacccagc tcgacgaact tcgccaacaa ggcctcttca agaccgaacg
     1621 cgtcatcgcg agcccccagg ccgggcagat tcgtgcgaac gacgccgacg tcatcaacct
     1681 ctgcgctaac aactacctgg gcctcgcgag ccatccggcc atccgcgaag ccgcccatcg
     1741 cgccatcgac cgctacggtt acggcatggc gtcggtgcgc ttcatctgtg gcacgcagac
     1801 ggtgcacaag cagctcgagg ccgcgctgtc ccgctttctc ggcaccgaag acaccatcct
     1861 ctattcatcg tgctgggacg ccaacggcgg tctgttcgaa accgtgctcg gcgaacagga
     1921 cgccatcatt tcggatggcc tgaaccacgc cagcatcatc gacggcatcc ggctgtgcaa
     1981 agcgcggcgc ctgcgctacg ccaatcgcaa tctcgccgaa ctcgagcagc atctgaagga
     2041 cacgcaggat tgccgcacgc ggctcatcgc caccgacggc gtgttctcga tggacggcag
     2101 catcgcgccg ctgcgcgaga tctgcgatct cgccgacaaa tacggcgcac tggttatggt
     2161 cgacgactcg cacgccaccg gcttcatggg ggccggcggt cgcggcacgc cagaacactg
     2221 cggtgtcgct tcgcgcatcg acttcctcac aggcaccttc ggcaaggccc tgggtggcgg
     2281 cagcggcggc tacacgtcgg gcaaggccaa cgccatcgcc tggcttcgac agcgctcgcg
     2341 cccttacctg ttctcgaaca gcattccgcc cgtggtcgcg gccgtcacgc tgcgcgcgct
     2401 cgatctcgtg gagaacatgc ctgacttgcg cgcgacgctg ttcggccatg cacgcaagtt
     2461 ccgggcgggc atgcaggcgg ccggcttcaa actactgccc ggcgagcacc ccatcatccc
     2521 ggtgatgatc ggcgacgcgg cattggccgg acaattcgcc gaccggctgc tgggcgaagg
     2581 cgtgtacgtc atcggtttct cattccccgt ggtcccgaag ggcgaagcgc gcatccgcac
     2641 gcagatgtcc gcggggctca atgacgcgca gctcgaacgt gcgcttgccg ccttcatcaa
     2701 ggtgggccgc gaattggagc tcatcaaatg aaggcactgg tcaagagcga agcaaaaccc
     2761 ggcatctgga tgaaggacat tcccgagccc acggtcggtc ccaacgacgt gctcatcaag
     2821 atcgccaaga ccgcgatctg cggcaccgac atgcacatct accactggga tgcgtgggcg
     2881 cagaaaacca ttccggtgcc catggccgtg ggtcacgagt atttcgggcg catcgtcgac
     2941 atgggcagtg aagtgcgcgg gttcgccatc ggcgatcgcg tgtccggcga aggccacctc
     3001 acctgcggct tctgccgcaa ctgccgcgcc ggccgccgcc acctgtgccg caacaccgtg
     3061 ggcgtgggcg tcgaccgcca gggatgtttc gcggaactgc tggtcatccc cgcgagcaac
     3121 gcgttcaagc tcccggacgc cgtcaccgac gacatcgcca gcatcctcga tccgttcggc
     3181 aacgccacgc acaccgccct cgcgttcaac atggtgggtg aggacgtgct gatcaccggc
     3241 gccggaccca tcggcatcat ggcggtcgcc atctcgaaga agatcggcgc gcgccacgtg
     3301 gtcatcaccg acgtcaacga ctaccgcctg ggtctcgcgc agaagatggg cgccacgcgc
     3361 gccatcaacg tcacgaaaca gacgctcgac gacaccatga atgagctcgg catgcaggaa
     3421 gggttcgacg tggggctcga gatgtcgggc aaccccgccg cgttccgcga catgctgcgc
     3481 accatgcatc acggcggcag cgtggccttg ttaggcatac cgccgggcga gacggccatc
     3541 gactggaacc aggtgatctt caagggcctc aacatgcgcg gcatctacgg ccgcgagatg
     3601 ttcgagacct ggtacaagat ggcggcactg ctgcaggccg gcctcgacat cacgccggtg
     3661 atcacgcatc acttcccgat ccaggaattc gagcagggtt tcaagaccat gggatcgggt
     3721 cagtcgggaa aggtcatcct cgactgggga acgctcagct aactacctaa cgtcgcagaa
     3781 gcagtaggc
//
LOCUS       DATDEU010000226         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1206263, whole genome shotgun sequence.
ACCESSION   DATDEU010000226 DATDEU010000000
VERSION     DATDEU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206263"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..832)
                     /locus_tag="VIV63_05580"
     CDS             complement(185..832)
                     /locus_tag="VIV63_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY6124099.1"
                     /translation="MRRRDFLSATSVLAASASLGGASSASAPASRTLSVFNNVSLDGY
                     FTDEAGDMSWAHDMDPEWTKFMSTNAGGDAELVYGRKTYEMMAAFWPSKEAAQAMPDV
                     ARGMNRMRKTVFSRTLKSVNWENSRLAQGDLVTEMRRMKAEPGPGLLILGSGEIVAQL
                     TQAGLIDSYQLVVVPIVVGRGRTLFEGVTGRPRLKLTQTREFSNGNVVCWYERVR"
     gene            949..2088
                     /locus_tag="VIV63_05585"
     CDS             949..2088
                     /locus_tag="VIV63_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY6124100.1"
                     /translation="MQTRQSLRFVSFVCVLLAHSALSQTTPPAPATPPARPAPPTRPA
                     DGPGAPPFTAITGKGANAPINKNGDFVIGPDYAPAPELNVVEGVPQGKIQQFVMDSKD
                     SKFYPGIAREVFGTVDPNNPKTLIVETHAKPYLRTITVYVPAQYKSGKKAPFIVTHDG
                     PALDKVDPALPRILDNLIAQKRVPAMIAVMIQNGGGDAQGSQRGLEYDTMSGKFAEFI
                     EAEVLPAVEKMADVKLTRKASGRAVMGCSSGAAAALSMAWNHPEWYSKIISYSGTYVN
                     QQWPFNQATPGGAWGYHESIIPNAKAKPLRIWMHVGDRDLYNPNVMRDDMHDWVEANH
                     RMAKVLKTKGYDYQYVYALDSGHCDKKVREQTLPQALEWVWRGYR"
     gene            2088..>2294
                     /locus_tag="VIV63_05590"
     CDS             2088..>2294
                     /locus_tag="VIV63_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124101.1"
                     /translation="MNFNKVWRLALLPALASFTLLGMWTTASAEAKVAPEFLKIFADW
                     KAASDAHDINKIMSVFDKSVVLSFQ"
BASE COUNT          461 a          732 c          705 g          396 t
ORIGIN      
        1 ctcattcttc ggctcatcga accaccgctc gaccgcgcgc ggaatacgaa tgctggagtc
       61 cgacacgcgc gcaccgaaga tctcaacgaa ctggtcgatg tccatctccg cgccggtctc
      121 gcggagatac ttcatatacg cggcctggat ctgggctgag tagcacatgg ggcgcattgt
      181 ggcctcaccg gaccctttca taccagcaca cgacgttgcc gttcgaaaac tcgcgcgtct
      241 gcgtgagctt gagccgcggc ctgccggtga cgccctcgaa caacgtacgt ccgcgcccga
      301 caacaatagg cacgacaacc agctggtatg aatcgatcaa ccccgcctgc gtgagctgcg
      361 cgacgatctc gccgctgccc aggatcaaca accccggccc gggctcagct ttcatcctgc
      421 gcatttcggt cacgaggtca ccctgcgcca gccgcgagtt ctcccagttc accgacttga
      481 gcgtccgcga gaacacggtc ttgcgcatgc ggttcatgcc gcgcgcaacg tccggcatgg
      541 cctgcgccgc ttctttcgag ggccagaacg cggccatcat ctcgtaggtc ttgcggccgt
      601 atacgagctc ggcgtcgcca cccgcgttgg tcgacatgaa cttcgtccac tcggggtcca
      661 tgtcgtgcgc ccagctcatg tcgccggcct catcggtgaa atacccgtcg agcgaaacat
      721 tgttgaacac ggacagcgtg cgtgacgccg gcgcgctggc gctcgacgcg ccaccgaggc
      781 tggcggatgc cgcgagcacg gaagtggccg acaggaaatc tcgacgacgc atgggattct
      841 ccaggctttc gaatccggat tggattcatg gcctaaacga acgggaacgc gcggattcga
      901 cagccaggcg ttacgatacg gcgcgcattt tttacgaggg gaatccgcgt gcagaccagg
      961 caatcattgc gttttgtgag tttcgtttgc gtgctgctgg cgcattccgc gctgtcgcag
     1021 accacgccgc ctgcaccggc caccccaccc gctcgccccg ctccgccgac gcgtcccgcg
     1081 gacggtccag gcgctccgcc attcacggcc atcaccggca agggcgcgaa cgcgcctatc
     1141 aacaagaatg gcgatttcgt catcggtccg gactacgcgc ccgcccccga gctcaacgtc
     1201 gtggaaggcg tgccgcaggg caagatccag cagttcgtca tggactcgaa ggacagcaag
     1261 ttctatcccg gcatcgcgcg cgaggtgttc ggtacggtcg accctaacaa cccgaagaca
     1321 ttgatcgtgg agacacacgc caagccctat ctacgcacga tcacggtcta tgtcccggcg
     1381 cagtacaaat caggcaagaa ggcgccgttc atcgtcacgc acgacggccc cgcgcttgac
     1441 aaggtagacc cggcgctgcc gcgcatcctc gacaacctca tcgcgcagaa gcgcgtgccg
     1501 gcgatgatcg ccgtgatgat ccagaacggt ggcggcgacg ctcaagggtc acagcgcgga
     1561 ctcgagtacg acacgatgtc cggcaagttc gcggagttca tcgaggccga ggtgctgccg
     1621 gcggtcgaga agatggccga cgtaaaactc acgcgcaaag cgagcggccg cgcggtcatg
     1681 ggctgcagct cgggcgcggc ggcggcgctg tcgatggcct ggaaccatcc ggagtggtac
     1741 tcgaagatca tcagctactc gggcacctac gtgaaccagc aatggccctt caaccaggct
     1801 acgccgggcg gcgcctgggg ttatcacgag tcgatcatcc cgaatgcgaa ggcgaagccc
     1861 ctccgcatct ggatgcacgt gggcgaccgc gatctctaca acccgaacgt aatgcgcgac
     1921 gacatgcacg actgggtgga ggctaaccac cgcatggcga aggtgctgaa gacgaagggg
     1981 tacgactacc agtacgtgta cgcgctggat tcagggcact gcgacaagaa ggtgcgggag
     2041 cagacgctgc cgcaggcgct ggagtgggtg tggcgaggct accgatgatg aatttcaaca
     2101 aggtgtggcg attggcgttg ctgccagcac tcgcgtcgtt cactctgctg ggcatgtgga
     2161 caaccgcaag cgcggaggca aaagtggcgc cggagtttct gaagatcttt gcggactgga
     2221 aagcggcatc cgacgcacac gacatcaaca agatcatgtc agtgttcgac aagtctgtgg
     2281 tcctgagctt tcag
//
LOCUS       DATDEU010000227         3824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2082067, whole genome shotgun sequence.
ACCESSION   DATDEU010000227 DATDEU010000000
VERSION     DATDEU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082067"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..829)
                     /locus_tag="VIV63_05595"
     CDS             complement(128..829)
                     /locus_tag="VIV63_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY6124102.1"
                     /translation="MLDALPELERERLFPHLKLVAMPLGMVLYESGAALRHIYFPTNS
                     IVSLLYVMQDGASAEIAVVGNEGAIGVSLFMGGETTPSRAIVQSAGHAYRLTGKRLKQ
                     EFNRHGEMLHVLLRYTQSLITQMAQTAVCNRHHSLDQQLCRWLLLSLDRLTGNTLQMT
                     QELIANMLGVRREGVTEAAGKLQKLGVIKYVRGKITVLDRQHLERLSCECYAVVKRET
                     DRLLAPHQVERPEAR"
     gene            1202..2032
                     /locus_tag="VIV63_05600"
     CDS             1202..2032
                     /locus_tag="VIV63_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6124103.1"
                     /translation="MSVIINSLFAPLAKVASLVNGHHAVRAPAIVLPLQVPDARADEI
                     ATISHELRNSLSVVRNAARLLRSPVGADGVEGARILIERHVGQMTRHIEDLLDASHRD
                     VRKKVLRRTHIDLRTIVEFAVSAIAPDLARRGHNLVLSLPAEPLWVEADSARMEQVFS
                     NLLINAAKYTPDGGDIVLTMESLEKRASIVIRDSGIGIEPAVLLRVFDMFAQADAAAI
                     CAEGGSGIGLAVVRDLVEMHGGTVRAASAGLGLGSEFTVLLPAVWSKVEVISTREVEQ
                     "
     gene            2299..2781
                     /locus_tag="VIV63_05605"
     CDS             2299..2781
                     /locus_tag="VIV63_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124104.1"
                     /translation="MRTPAHAHADDTFSSGWRCDRGFRQNQDRCEAIAVPVDGFAADS
                     SFGRGWDCVRGFQAVGDTCVRLQIPEHGYLLSSGNEWGCERGYDRHNGKCVAVIVPAN
                     GYLDSRGTDWRCERGFGRNANTCQALVLPDNSHIDYTGNDWTCLDGFRRQSGACLPRQ
                     "
     gene            2901..3305
                     /locus_tag="VIV63_05610"
     CDS             2901..3305
                     /locus_tag="VIV63_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEY6124105.1"
                     /translation="MNFQFKRASITRRRFRIALPALLMFAGAAMAAPPIPVTNIQAAE
                     LAIANAERVDAATHAGVELGEARGKLAAAQNAVQDKKMVVAQQFADEARAGAELAAAK
                     AGAVKALAVNEDIKRSTATLVEEMQRKSGDTR"
     gene            3302..>3824
                     /locus_tag="VIV63_05615"
     CDS             3302..>3824
                     /locus_tag="VIV63_05615"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEY6124106.1"
                     /translation="MNNSKNFPIRSMIAVAVATALIAGCASAPMKPAGSAEVRAKLTA
                     LQSDPSLANSAPVAMKDAETAVTVAEMVQKDLALAAHRVYIANRKVDTARALAQTQTA
                     ETERKALTEAGEKARLNARTLEADKAKNDALVARAESAEQKLIAGQARENADASQLAA
                     NASQQQALELQRQL"
BASE COUNT          700 a         1203 c         1213 g          708 t
ORIGIN      
        1 gagtttggcg gcgacggccg gattcgcacg gcgaccggcg tcgcggagca cacgcactca
       61 gtcgcgcgaa cgggccgcgc gcctgtcttt cactaccgga tttcgcactg gatccaggcg
      121 aacgcgctca cctcgcctca gggcgttcca cctgatgcgg agccaacagg cgatcggttt
      181 cgcgcttgac cacggcataa cattcgcagg acagccgctc gagatgctgc ctgtccagca
      241 cggtgatctt gccgcgcaca tactttatga cgcctaactt ctgcagcttg cccgccgcct
      301 cggtgacgcc ttcgcgccgc acgccgagca tgttggcgat gagttcctgg gtcatttgca
      361 gcgtgttgcc ggtcagccga tccagcgaga gcaggagcca gcggcacaat tgctggtcga
      421 gcgaatgatg gcggttgcaa accgcggtct gcgccatctg ggtgatcagc gactgcgtgt
      481 agcgcagcag cacgtggagc atttcaccgt gtcggttgaa ttcctgcttc aggcgcttgc
      541 cggtcagccg gtaagcgtgg ccggcgcttt gcacgatggc ccggctgggc gtggtttcgc
      601 cgcccatgaa taaagagacg ccgatggcgc cttcgttacc gaccacggca atctccgccg
      661 aagcgccgtc ttgcatgaca tacagcagcg atacgattga attcgttggg aagtagatgt
      721 ggcgcagcgc agcgcccgac tcgtacagca ccatgccgag gggcatggca accagcttca
      781 ggtgaggaaa cagccgctca cgttcgagct ctggcagtgc atcgagcacg tgattctggc
      841 gcggatcctg gggcttgttc attgacctgc aatctacgct ctatcgcgcg gctttgtacg
      901 ctaccgctca caccggcccg ggaagtgccg ctgtgtacgg agacgcacag accgccagcc
      961 ttacatccac gtaggcttcc gccgatgcac ggtaaaccaa taagaatcgc tatctggggg
     1021 ctcgcaatca tcgttagctc acttctctac atcaactcgt cgctgctgcc tcacccggag
     1081 ggcggcccgt ggctggcttt gagcggaatc cttttcgtac tggctgcagt tgcactgggt
     1141 gcgcgccgac accttccatt cggtgggcga atctgaaaac caactcgttt cggagagcgt
     1201 cgtgtctgta atcatcaact ctctattcgc accgctggcc aaagtcgcct ctctggtcaa
     1261 cggccatcac gcggttcgtg cgccggccat cgtccttccg ctgcaagtgc cggatgcgcg
     1321 tgccgatgag attgcgacca tctcgcacga gttgcgcaat tcgctgagcg tggttcgtaa
     1381 cgccgcgcgg ttgttgcgtt cgccggtcgg ggccgatggc gtcgaaggcg cgcgaatcct
     1441 catcgaacgc cacgtgggcc agatgacgcg gcacatcgaa gacctgctcg acgcctcgca
     1501 ccgggatgtt cgcaagaaag ttctgcggcg cacgcacatc gatctgcgga ccatcgtgga
     1561 atttgccgtc agcgccatcg cgccggacct ggcgcggcgc ggccataacc tggtgctcag
     1621 cctgcctgcg gagccgctgt gggtggaagc tgattccgcg cgcatggaac aggtgttttc
     1681 gaatctgctg atcaacgccg cgaagtacac gccggatggc ggcgatatcg tgctgaccat
     1741 ggagagtctc gaaaaacgcg ccagcatcgt cattcgcgac tccggcatcg gcatcgagcc
     1801 tgccgtgctg ttgcgcgtgt tcgacatgtt cgcccaggcg gatgcggcgg cgatttgcgc
     1861 cgagggtggc agcggaatcg gccttgccgt ggtgcgggac ctcgtcgaaa tgcatggtgg
     1921 caccgtgcgg gcagcgagcg ctggccttgg cctgggcagt gagttcaccg tacttctgcc
     1981 ggccgtatgg tcgaaggtgg aagtgatttc aactcgcgaa gtcgagcaat gaaaaaagcg
     2041 ccgcgattgc gcgcttcaat tccgctgctc gcgcttctgg tcttcaccgc cgcgtctgct
     2101 gcgagtggaa atgcagagcg tcccgccaat tccgtccagc gccaatccgg cgtcggttgg
     2161 gattgtgcgc ggggatttcg caagtccgcg gatctttgtc aggcgatcgc cgtacccgcg
     2221 aatggctttc tcagtttcag tggaaccgac tggacctgcc accggggcta cctcaagacc
     2281 acgcgaggtt gcgaagcggt gcgaaccccg gcgcacgcgc acgctgacga caccttcagc
     2341 tccggctggc gttgcgatcg tggcttccgg caaaaccagg atcggtgcga ggcgattgcc
     2401 gtgcccgtcg acggctttgc cgcggactcc tctttcggtc gcggctggga ttgcgtgcgc
     2461 gggttccagg ccgtaggtga cacgtgcgta cgcctgcaga ttccggaaca cggctatcta
     2521 ctgagttccg gaaacgagtg gggatgcgag cgcgggtatg accggcacaa cgggaagtgc
     2581 gtggcggtga tcgttccggc caatggctac ctcgacagcc gcggcacgga ttggcgctgc
     2641 gaacgtggct tcggccgcaa cgctaacacc tgtcaggcgc tggtactgcc agacaactcc
     2701 cacatcgatt acacgggcaa tgactggacc tgtctggacg gttttcggag gcaatccggc
     2761 gcctgtctgc cacggcaata gtggcctaac aaggttatat gttcggttcc gcaccgacag
     2821 atgtgcccgc acgcgtgcac actgcgttct ctggtggatg tcgccccggt gtaatccatc
     2881 gtcatcagta gagaacgtca atgaatttcc agttcaagag agcgagcatc acacgacgcc
     2941 gcttccgcat cgcgttgccc gcgttgctca tgtttgccgg cgccgccatg gccgcgcccc
     3001 cgattcccgt caccaacatt caggccgccg agctggcgat tgcgaatgcg gagcgcgtcg
     3061 atgccgccac ccatgcgggt gtcgagctcg gtgaagcccg gggcaagctg gccgctgctc
     3121 agaacgcggt ccaggacaag aagatggtcg tcgcgcagca gtttgccgac gaagcgcgcg
     3181 ccggcgcgga actggccgcc gctaaggccg gtgcggtcaa agccctcgca gtgaacgagg
     3241 acatcaagcg cagtaccgcg acgctggtgg aagaaatgca gcgcaagtca ggagataccc
     3301 gatgaacaat tccaaaaact ttcccatccg ctcgatgatt gcggtcgcgg tcgcgacggc
     3361 ccttatcgcc ggctgcgcct cggcgcccat gaaacccgcg ggttccgctg aggtgcgcgc
     3421 caagctcacc gcgctgcagt ccgacccctc gctcgccaac agcgcgccgg tcgccatgaa
     3481 ggatgccgag accgccgtga ccgtggccga gatggtgcag aaggatctcg cgctggccgc
     3541 gcatcgcgtg tacatcgcga atcgcaaggt ggacaccgcg cgagcgcttg cccagacgca
     3601 gaccgcggaa actgagcgca aggcgctcac cgaggcgggc gagaaggctc gtctgaatgc
     3661 acgcaccctg gaagcggaca aagccaagaa cgacgcgctg gttgcgcgcg cggaaagtgc
     3721 cgagcagaag ctcatcgccg gccaggcgcg tgagaacgcc gacgcgtcac agctcgccgc
     3781 caatgcctcg caacagcagg cgctcgaact gcaacgccaa ctcg
//
LOCUS       DATDEU010000228         2449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1061466, whole genome shotgun sequence.
ACCESSION   DATDEU010000228 DATDEU010000000
VERSION     DATDEU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061466"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VIV63_05620"
     CDS             <1..350
                     /locus_tag="VIV63_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEY6124107.1"
                     /translation="ARRYFRASPRTPVFPEDNGPPGIRHGRAGRQADGTAVFFELQIA
                     DGIVKSARFSAYGCPHTVAVVAWLCEVVEGSRLEAGIPGTPADWARQFDVPAEKLGRL
                     LIVEDALRAALNR"
     gene            373..696
                     /gene="iscA"
                     /locus_tag="VIV63_05625"
     CDS             373..696
                     /gene="iscA"
                     /locus_tag="VIV63_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEY6124108.1"
                     /translation="MAISLTPAAAERVKTFIASRGKGLGLRLGVRKTGCSGFAYVVNY
                     ADDAAAQDLVFEDQGVKVFVDPESLPLINGTTVDFVKQGLNEAFRFHNPNIKGECGCG
                     ESFNV"
     gene            809..1237
                     /gene="ndk"
                     /locus_tag="VIV63_05630"
     CDS             809..1237
                     /gene="ndk"
                     /locus_tag="VIV63_05630"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY6124109.1"
                     /translation="MALERTLSIVKPDGVARNLIGQVIGRFEKVGLKVVAARMVHLSQ
                     RDAEGFYAVHAARPFFKDLVKYMTSGPVLVQVLEGEGAILKNREVMGATDPKKAAPGT
                     IRADLAESIEANTVHGSDSAENAAIEIAYFFRTTELCPRG"
     gene            1245..2360
                     /gene="rlmN"
                     /locus_tag="VIV63_05635"
     CDS             1245..2360
                     /gene="rlmN"
                     /locus_tag="VIV63_05635"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY6124110.1"
                     /translation="MSAVQENPKTNLLGLPKAELEAFVDSMGSKPFRARQLMNWMYKR
                     GEGDIAAMTDLAKDFRAELVLRAEVAVPEIVKTQIASDGTRKWLLSGGNGQAYEMVYI
                     PEEDRGTLCISSQVGCALDCSFCSTAQQGFNRNLTTAEIVGQVWLANRELGWQVGDNR
                     IITNIVLMGMGEPLANFRNVVPAIRIFLDDLGFDISRRRVTLSTSGLVPQIYKLAEEV
                     NCALAVSLHAPNDDLRSQLVPINRKHNIAELLAACWHYLDEQNGRSVTFEYVMLDGIN
                     DRPEHARELAKLLRDKPAKLNLIPFNPFPGTHYRRSPAPAIEKFRDELMQRGIIVTVR
                     KTRGDDIDAACGQLAGQVTDRTIVRLGSKTFGLQVRA"
BASE COUNT          463 a          793 c          761 g          432 t
ORIGIN      
        1 aggcgcgccg ctatttccgg gcgtcgccgc gcaccccggt attccctgag gacaatgggc
       61 cgcctggaat ccggcacggg cgggcaggca ggcaggccga cgggacggcg gtatttttcg
      121 agctgcaaat cgccgatggg attgtgaaaa gcgcccgttt cagcgcgtac ggttgccccc
      181 atactgtggc tgtcgtggcc tggttatgtg aggtcgtgga aggctcccgg ctcgaggccg
      241 gcattcccgg tactcccgcc gactgggccc gccaatttga tgtcccggcc gagaaacttg
      301 gccggctgct gatcgtcgaa gatgcattgc gcgcggcgtt gaatcgctga cgcgtccagg
      361 aaagttcgca aaatggctat ttcgcttaca ccggctgcgg ccgaacgcgt caagaccttc
      421 atcgcctcgc gcggcaaggg actgggtctg cgtctgggtg tgcgcaagac aggctgctcg
      481 ggcttcgcct acgtcgtgaa ctatgccgac gacgccgcgg cccaggacct ggttttcgag
      541 gaccaggggg tcaaggtgtt cgtcgatccc gagagcctgc cgctcatcaa cggcaccacc
      601 gtggatttcg tcaaacaggg cctgaacgag gccttccgct tccacaaccc gaacatcaaa
      661 ggggaatgtg gctgcgggga atccttcaac gtctagcaag gcgggccgaa gttcctttcg
      721 gcgttgaatt tcagcaggtt actcgcgggg agggcgtccg cccggtacaa tcgcgcgcct
      781 tcaagacccc actcaaggaa tcattcgcat ggcgctcgag cgcactctgt ccatcgtcaa
      841 gcccgacggc gttgcccgta acctcatcgg ccaggtcatc ggccgcttcg aaaaagtcgg
      901 cctcaaggtc gtcgccgcgc gcatggtgca tctgtcgcag cgtgacgccg aaggtttcta
      961 cgccgtgcac gccgcgcgtc cgttcttcaa ggacctcgtg aagtacatga cctcgggtcc
     1021 cgtgctcgtg caggtgctgg aaggcgaagg cgccatcctc aagaatcgcg aagtcatggg
     1081 cgccaccgat ccgaagaaag ctgcgccggg caccatccgc gccgatctcg ccgagagcat
     1141 cgaagcgaac accgtgcacg gttcggactc cgccgagaac gccgcgatcg aaatcgcgta
     1201 cttcttccgc acgacggagc tctgcccgcg cgggtaacgc tcagatgtcc gctgtccagg
     1261 aaaaccccaa gacgaacttg ttaggtctgc ccaaggcgga gctcgaggcg ttcgtggatt
     1321 ccatgggcag caaacccttc cgcgcgcgcc agctcatgaa ctggatgtac aagcgcggcg
     1381 agggcgacat cgcggcgatg accgatctcg ccaaggactt ccgcgcggaa ctcgtgttgc
     1441 gtgcggaagt cgcggttccg gaaatcgtga agacgcagat tgcctcggac ggcacacgca
     1501 agtggctgct gtccggaggc aacggccagg cctacgaaat ggtctacatc cccgaggaag
     1561 atcgcggcac gctgtgtatc tcctcgcagg tgggctgcgc gctcgactgc tcgttctgtt
     1621 cgacggcgca gcagggcttc aaccgcaatc tcaccactgc cgagatcgtc ggccaggtct
     1681 ggcttgcgaa tcgggagctc ggctggcaag tcggtgacaa ccgcatcatc accaacatcg
     1741 tgctcatggg catgggcgag ccgctggcca acttccgcaa cgtggtgccg gccatccgca
     1801 tcttcctcga tgacctgggg ttcgacatct cgcgccgccg cgtgacgctg tcgacgtcgg
     1861 ggctcgtgcc gcagatctac aagctggccg aagaagtgaa ctgcgcgctg gccgtatccc
     1921 tgcacgcgcc caacgacgac ctgcgcagcc agctcgtgcc gatcaaccgc aagcacaaca
     1981 tcgcggagtt gttggcggcc tgctggcact atctcgacga gcaaaatggc cgcagcgtca
     2041 cgttcgaata cgtgatgctc gacggcatca acgaccggcc ggagcatgcg cgcgagctcg
     2101 cgaagctgct gcgcgacaaa ccggccaagc tcaatctcat tccgttcaat ccgttccccg
     2161 gaacgcacta tcgccgctct cccgcgccgg cgatcgagaa gttccgcgac gaactcatgc
     2221 aacgcgggat catcgtcacc gtccgcaaga cgcgcggcga cgacatcgat gcggcctgcg
     2281 gacaactcgc gggccaggtc accgaccgca ccatcgtgcg gctgggttcg aaaaccttcg
     2341 gattgcaggt gcgcgcatga acgccagatg gatcctcgtc ctttcggtct gcctgttcgc
     2401 cggatgtgtc accaccgacg gcagcaagcc gcgcaagcag gaagacccg
//
LOCUS       DATDEU010000229         2686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1206836, whole genome shotgun sequence.
ACCESSION   DATDEU010000229 DATDEU010000000
VERSION     DATDEU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206836"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="VIV63_05640"
     CDS             complement(<1..1465)
                     /locus_tag="VIV63_05640"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HEY6124111.1"
                     /translation="MNAAAASLPTSESLRDSGARLVTPELIVFPVRHHSPACAWHLRA
                     LMDSHQPSVVLVEGPRSFTPLMPLLTHPDARMPLAIYTYVVQRAKGDEAPPRRASYFP
                     FCDYSPELVALRAAQELGIPARFIDLDFAEQCQIEPDDGDADSNSLLDERHLERSAHL
                     RLLAQQLGCRNHEELWEHLFECDAASRDALVHVANVAAYCQLARLDNTEEELRVDGTL
                     AREAEMAWHIREALKARERGAGPVLAVVGGFHAVVLPELVAGDTPRPQISRSAVSEEN
                     SALIRYSFDRLDRLNGYASGMTSPAWHQLLWERQLKLERIRTAASPRVRQDAALSVLF
                     DIAELLRDKHRVPIPVPTMAAAFRHALELARLRRRPSPVRDDVVDAVTSCFVKGDIEA
                     EGPVIFGAVHQVLTGVVTGKVPPGAPKPPLLRDFEYRARRQRLKIDDAEPRRSLLDIY
                     RRPEHRVTSRLLHGLVLLGVPFAFRTAGPDFVNGTGLD"
     gene            complement(1471..2562)
                     /locus_tag="VIV63_05645"
     CDS             complement(1471..2562)
                     /locus_tag="VIV63_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6124112.1"
                     /translation="MPTDILRAPAEQTFAGELARLAEKDKESKPTGWKLSPRAVRSFI
                     LGEPKLDVRRKFFGDDALIDRCVVTLMSDRGLLLVGEPGTAKSMLSELLSAAISGQSI
                     CTIQGTAGTTEDQIKYSWNYALLLAEGPSPRALVPGPIHEAMSTGCLCRFEEVTRVQP
                     EIQDCLISLMSDKLLHVPELDGDAATVFAARGFNILATANIRDRGVHEMSSALKRRFN
                     FETVRPIADRKLEIQLVREQTDSLLKRADIEVDLQPDVIELLVTAFHDLRAGTTAEGV
                     VVEKPTAVMSSAEAVSVGYAACLDAHYLGDGTVNGEHIARQLIGTVLKDNPDDGKKLG
                     HYFDVVVKQRAQRQPQWKRMLEARRELNR"
BASE COUNT          440 a          858 c          922 g          466 t
ORIGIN      
        1 ggtcgaggcc cgtgccgttc acgaagtcgg gccccgcggt gcggaacgcg aacggcacgc
       61 ctaacaacac gagtccgtgg agcagccggc tggtcacccg atgttcgggc cggcggtaga
      121 tgtcgagcag cgaccggcga ggctcggcgt cgtcgatctt gaggcgctgc cgccgcgcgc
      181 ggtattcgaa gtcgcgcagc agcggcggtt tgggcgcgcc cggcggcacc ttgcccgtga
      241 cgacaccggt gagcacctgg tgcaccgcgc cgaagatcac cgggccttcg gcttcgatat
      301 cgcctttcac gaagcagctc gtgacggcat cgaccacatc gtcacgaacc ggggagggac
      361 ggcggcgcag cctcgcgagc tcgagcgcgt gccggaacgc tgcggccatc gtcggcaccg
      421 ggatcggcac gcggtgtttg tcgcgcagca gttccgcgat gtcgaacaac acggacagcg
      481 ccgcatcctg gcgcacccgc ggactggccg cggtcctgat gcgttcgagc ttgagctgac
      541 gttcccacag caactggtgc cacgctggcg acgtcatgcc cgaggcatat ccgttcagtc
      601 ggtctagccg gtcgaagctg taacggatga gcgcggaatt ttcctcgctg accgcagacc
      661 gcgagatctg cggacgcggt gtgtcgccgg ccacgagttc cggcaacacc acggcgtgaa
      721 atccgccgac cacggccagt accggtcctg cgccgcgctc acgcgctttc aacgcttcgc
      781 gaatgtgcca ggccatttcc gcctcgcggg cgagcgtgcc gtcgacgcgc agctcttctt
      841 ccgtgttgtc gaggcgtgcg agctggcagt acgcggccac gttcgccacg tgaacgagcg
      901 cgtcgcgaga cgccgcatca cattcgaaca ggtgctccca gagctcttcg tgattgcggc
      961 agccgagctg ttgcgcgagc agccgcagat gcgcgctgcg ctcgaggtgc cgctcgtcga
     1021 gcagcgagtt cgaatccgcg tcgccgtcgt cgggctcgat ctggcactgc tcggcgaagt
     1081 cgagatcgat gaagcgcgcg ggaatgccga gctcctgcgc cgcgcgcagc gccacgagct
     1141 cgggcgagta gtcgcagaac ggaaagtagg acgcgcgccg cggcggcgct tcgtcacctt
     1201 tcgcgcgctg gaccacgtag gtgtagatgg cgagcggcat gcgcgcatcg gggtgagtga
     1261 gcagcggcat caatggagtg aacgagcgcg gaccttcgac cagcacgacg gacggttggt
     1321 gcgaatccat caacgcgcgc agatgccagg cgcaggccgg gctgtgatgc cgcacgggaa
     1381 agacgatgag ctcgggcgtc acgagccgtg cacccgaatc tcgcagggat tcggaggtcg
     1441 ggagactggc ggccgcggcg ttcacggcgc tcaacgattc aattcacgcc gtgcctctaa
     1501 catgcgcttc cattgcggct gccgctgggc gcgctgcttg accaccacgt cgaagtaatg
     1561 gcccagcttc ttgccgtcgt cggggttgtc cttgagcacc gtgccgatga gctggcgcgc
     1621 gatgtgttcg ccgttcaccg tgccgtcgcc gagatagtgc gcgtcgaggc aggccgcata
     1681 acccaccgag acggcttcgg ccgacgacat gaccgcggtg ggtttctcga cgaccacgcc
     1741 ttcggccgtg gtgccggcgc gaagatcgtg gaaagccgtg accagcagtt cgatgacgtc
     1801 gggttgcagg tccacttcga tgtcggcgcg cttgagcagg ctgtcggtct gttcgcgcac
     1861 gagctggatt tcgagtttgc ggtcggcgat cggccgcacc gtctcgaaat tgaagcggcg
     1921 cttgagcgcg ctcgacatct cgtgcacgcc gcgatcgcgg atgttggcgg tggcgaggat
     1981 gttgaaaccg cgcgcggcga acaccgtggc ggcgtcgccg tcgagctcgg gtacgtgcag
     2041 cagtttgtcg gacatgagac tgatcaggca gtcctggatc tccggctgca ctcgcgtcac
     2101 ttcctcgaag cggcagagac aaccagtgct catggcttcg tgaatgggac cgggcaccag
     2161 cgcgcgtggc gaagggccct cggccagcag cagcgcgtag ttccagctgt acttgatctg
     2221 gtcctcggtc gtgccggccg tgccctggat ggtgcagatc gactgtccgg aaatggcggc
     2281 ggagagcagc tccgacaaca tggacttcgc ggtgccgggt tcgccgacca gcagcaggcc
     2341 gcggtcgctc atgagcgtga ccacgcaacg gtcgatgagc gcatcgtcgc cgaagaattt
     2401 gcgccgcaca tcgagcttgg gctcgccgag gatgaaactg cgcaccgcgc gcggcgagag
     2461 cttccagccg gtgggtttcg attccttgtc cttctccgcg agccgcgcga gttcgcccgc
     2521 gaaagtttgc tcggccggtg cgcgcaggat gtcagtaggc actcttcttc tcccagtccg
     2581 gatcgaaacc ggtgccgtcc gcggccatga gccgcatgtc gttgtaacac tcggacagca
     2641 gcactttcgg aatcttcttg agcggaatct cgctgcgctc ccagct
//
LOCUS       DATDEU010000230         3994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_914958, whole genome shotgun sequence.
ACCESSION   DATDEU010000230 DATDEU010000000
VERSION     DATDEU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914958"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1714)
                     /locus_tag="VIV63_05650"
     CDS             complement(83..1714)
                     /locus_tag="VIV63_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124113.1"
                     /translation="MSTNEFFEIRNIRLLLVEDNPADARVVERHLKDAGLNQVACDWV
                     QSAAAASERLQKVEYDLVLLDLGLPDAQGLEALRALRAVADLTPIIVLTGSDDYKQGL
                     SAVREGAQDYLEKRRVNAGMLSRIVSYSVERNNFHRDLVRATRRYQDLFNNVPVALFT
                     ANQDGVLKTANAAFVKLLSCQGIPLDKVNFLDLLFEPGKRATFLDQIAKHRAVEHWET
                     SIQDCKGGKLNVLINTAPLYDATQAFAGWEGCITDISMLKQTLEERDRLEDNLRQAQK
                     LEAIGQLAAGIAHEINTPTQYVGDNLRFLKESFGELDGLLTQLVEAGGAPAAKLIGEA
                     DFEYLKEEIPRALNQSLEGVDRVAKIVRAMKEFSHPAREKTATDLNRAIQSTITVASN
                     EWKYVAEIEMDLDTGLPAVHCSPAEFNQVVLNIVVNAAHAISDVVGDGGKGKGKIRVR
                     TRADGDFAVIEITDSGSGMPPHVQQRIFEPFFTTKEVGKGTGQGLAIAHNVIVDKHGG
                     TIKVASSPGAGTTFTIRLPIGGSKVEAEPVAGATA"
     gene            2030..3055
                     /locus_tag="VIV63_05655"
     CDS             2030..3055
                     /locus_tag="VIV63_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124114.1"
                     /translation="MTNGPRDALFVAHPVPSLLFDRQYLVVAANTAAQTLFRCSAAEI
                     EGFDMVTFFPELGASLRATRQVTAMRSVAVRRPDGSNFIARLQLVVPGADALTLATIE
                     DMTEHEQELSAANKEFETFTSAAGHDLRGPLRILKGFTEALEDECGESLNEEGKSFLK
                     EILKASDRMEGLIDGLLALSRANRVDMACEKLDLTTLVELVTYELRHGKSAREVECTV
                     DPGINGWGDVRLMMTALRILFGNAWKYTSRIDNGKIRFHAEERDGRKWYCVTDNGAGF
                     DMSHASRLFQPFIRLHRQDEFPGHGIGLATVQRIVKRHGGEIEAQSAVNQGTTIRFWL
                     PPTPHGR"
     gene            3225..>3994
                     /locus_tag="VIV63_05660"
     CDS             3225..>3994
                     /locus_tag="VIV63_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124115.1"
                     /translation="MRGKTSALLRYLTAPFLAALGGHAFAQSADTTAAPTAPYLVDAS
                     QISTGDTSWMLTSTALVLLMTLPGLALFYGGMVRKKNLLSTMAQSFAIACLVTVLWVV
                     IGYSLAFTESSPWLGGTTRFALEGLFYAKGGQVSVSHLAPTIPESVFMMFQMMFAIIT
                     PALITGAFAERIKFSSMLVFMTLWSLCVYVPMAHWVWSPDGWLASLGVLDFAGGTVVH
                     INAGVTGLACVIMLGERKGYGTDPIFPHNLALTITGAAL"
BASE COUNT          664 a         1254 c         1288 g          788 t
ORIGIN      
        1 tcgttgcgct tgggccgaag catgcgcttc aggccctcga gcacgcgtgg ttcgtcgtcg
       61 acgaagatga cgcggattgc gttcaagcag tggccccggc aaccggctcc gcttccactt
      121 tcgagccgcc gatgggcagg cggatggtga acgtagtgcc agcgcccggg ctgctcgcca
      181 ccttgatcgt gccgccgtgc ttgtcgacga tcacgttgtg ggcgatggcc aggccctggc
      241 ccgtgccctt gccgacttcc ttggtggtga agaacggctc gaagatgcgc tgctgcacgt
      301 gcggcggcat gccggagccg gagtcggtga tctcgatgac ggcgaaatcc ccgtcggcgc
      361 gtgtgcgcac gcggatcttg cctttgccct tgccgccgtc gccgaccacg tcggagatgg
      421 catgcgcggc gttcacgacg atgttgagca ccacctggtt gaactcggcc ggtgagcagt
      481 gcacggcggg caggccggtg tcgagatcca tttcgatctc ggcgacgtac ttccactcgt
      541 tgctggccac cgtaatggtg ctctggatcg cccggttcag atcggtggcg gtcttctcgc
      601 gcgccgggtg cgagaactct ttcatggcgc gcacgatctt ggccacgcgg tcgacgcctt
      661 cgagagactg gttgagcgcg cggggaattt cttccttgag atattcgaag tcggcttcgc
      721 cgatcagctt ggctgccggc gcgccgccgg cttcgacgag ctgcgtcagc aggccatcga
      781 gttcgccgaa cgactccttg aggaatcgca ggttgtcgcc cacgtactgc gtcggcgtgt
      841 tgatttcgtg cgcgatgccg gcggcgagct ggccaatggc ttcgagcttc tgcgcctggc
      901 gcaggttgtc ttccagccgg tcgcgttcct cgagcgtctg cttgagcatc gagatgtcgg
      961 tgatgcagcc ttcccaaccg gcgaaggcct gggtggcgtc atacaacggg gcggtattga
     1021 tgagtacgtt gagcttgccg cccttgcagt cctggatcga agtttcccag tgctcgaccg
     1081 cgcggtgttt ggcgatctga tccagaaacg tcgcgcgttt gccgggttcg aacaaaaggt
     1141 cgaggaagtt gaccttgtcg agcggtatgc cctggcagga aagcagtttc acgaacgcgg
     1201 cgttggcggt cttgaggacg ccgtcctggt tcgcggtgaa cagtgccacg ggcacgttgt
     1261 tgaagagatc ctggtaacgg cgcgtggcgc gcaccaggtc gcgatggaaa ttgttgcgct
     1321 ccaccgaata cgaaacgatg cgcgagagca tgccggcgtt gacgcggcgc ttctcgagat
     1381 agtcctgcgc gccttcgcgc accgcggaca ggccctgctt gtagtcgtcg gaaccggtga
     1441 gaacgatgat gggcgtgaga tccgccacgg cacggagcgc gcgcagcgct tcgaggccct
     1501 gcgcatcggg aagcccgaga tcgagcagca ccaggtcgta ttcgactttc tgcagtcgct
     1561 ccgatgcggc ggccgcggac tgcacccagt cgcacgcgac ctggttgagt ccggcgtcct
     1621 tgaggtgacg ctcgaccacg cgggcgtcgg ccgggttgtc ctcgactaac aacaaccgga
     1681 tattcctgat ctcgaagaat tcgttggtgc tcatcgcgcg gtttctacac ttggtgttgc
     1741 ggggatgtga tcaatcggtg cggcgcgtac atcccggcgt tacgccacgc tcagtaatat
     1801 cggcccgtgg agccaatacc ttaggcggcg gcgacgcatt gccgcctgtc gcgatgccaa
     1861 tcacatatcg gccgcaaacc gtgcgcctaa accctgcggg ggccgaatct gggcgcctgc
     1921 tcacaaagaa ttcacccgcg cctatccaga ctcggcggcg cgtgccgatt tcatttcccg
     1981 acacagtctt atgacgcaac tcgcagaaga aaatctggcg gacacgacct tgacgaacgg
     2041 gcctcgtgat gcgctgttcg tcgcgcaccc cgtgccgtcg ctgctgttcg atcgccaata
     2101 cctggtcgtt gcggccaaca ccgccgcgca gacgctgttt cgttgcagtg cggcggagat
     2161 cgaaggtttc gacatggtga ccttctttcc cgagctcgga gcctcgctgc gggcgacgcg
     2221 ccaggtcacc gccatgcgca gcgtggcggt gcgccggccg gatggcagca acttcatcgc
     2281 gcgacttcag ctcgtggtgc cgggcgccga tgcgctgacg ctcgccacca tcgaggacat
     2341 gaccgaacac gagcaggagc tctctgcggc gaacaaggag ttcgagactt tcacctccgc
     2401 ggccggccac gatctgcgcg gaccgttgcg tatcctcaag ggattcaccg aagcgctcga
     2461 agacgaatgc ggcgagtcgc tgaacgagga gggcaagtct ttcctcaagg agatcctcaa
     2521 ggccagcgat cgcatggaag gactcatcga tggcctgctc gcgctgtcgc gcgccaatcg
     2581 cgtcgacatg gcctgcgaaa aactggatct gacgacgctg gtggagctcg tgacctacga
     2641 gcttcgacac gggaaatccg cgcgtgaagt cgaatgcacg gtggatcccg gcatcaacgg
     2701 ctggggcgac gtgcgcctca tgatgacggc gctgcgcatc ctgttcggca acgcgtggaa
     2761 atacaccagc cgcatcgaca acggcaagat ccgtttccat gccgaagagc gcgacggccg
     2821 caaatggtat tgcgtgaccg acaacggcgc gggtttcgac atgtcgcatg ccagccgtct
     2881 cttccagccg ttcatccgtc tgcatcggca ggacgaattt ccggggcacg gcatcgggct
     2941 cgccacggtt cagcgcatcg tgaaacgcca cggcggcgag atcgaggcgc agtcggcggt
     3001 gaaccagggc acgacgatcc gcttctggct tccgccgacg ccgcacggcc gctgacgcca
     3061 gtgagaactg gcgcacatcg cgcctctttt cacggcatcc gggcgcgccg gcgtggtgcg
     3121 cactgcggca gatgtgtttt gttagacata gcgcacattc ggaaatccgt ttcgtggtgg
     3181 caagcgcaaa gtcctagggc ttgccatttg ccagaatggc gcgcatgaga ggcaaaactt
     3241 ccgcacttct ccgttacctg acggccccgt tcctggccgc gctgggcggt cacgcgttcg
     3301 cgcagtccgc ggatacgacg gctgccccga cggcgcctta cctcgtcgat gccagccaga
     3361 tcagcacggg cgacacgtcc tggatgctca cctccacggc gctggtgttg ctcatgacct
     3421 tgccgggcct cgcgctgttc tacggcggca tggtgcgcaa gaagaacctg ctgtcgacga
     3481 tggcgcagag tttcgccatc gcctgtctcg tcaccgtgct gtgggtcgtc atcggctaca
     3541 gcctcgcgtt caccgagagt tcgccgtggc tcggcggcac gacgcgcttc gcgctcgaag
     3601 gactcttcta tgcgaagggc ggccaggtgt cggtcagcca tctcgcgccg accatccccg
     3661 aatccgtctt catgatgttc cagatgatgt tcgccatcat cacgcccgcg ctcatcaccg
     3721 gcgcgttcgc cgagcgcatc aagttttctt cgatgctcgt cttcatgacg ctgtggtcgt
     3781 tgtgcgtgta cgtgccgatg gcccactggg tgtggtcgcc ggatggctgg ctggcgtcac
     3841 tcggcgtgct cgacttcgcg ggcggcaccg tggtgcacat caacgccggc gtcaccggtc
     3901 tcgcctgcgt gatcatgctg ggtgagcgca aaggttatgg caccgatccg atcttcccgc
     3961 acaacctcgc gctcaccatc acgggcgcag cgct
//
LOCUS       DATDEU010000231         8678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1498860, whole genome shotgun sequence.
ACCESSION   DATDEU010000231 DATDEU010000000
VERSION     DATDEU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498860"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VIV63_05665"
     CDS             <1..210
                     /locus_tag="VIV63_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124116.1"
                     /translation="PFVDLLLPVDLGATRGVVLWVKFIIELCYFIFAIHTFGKLPLWN
                     TVLRCILVLGAGNALLIFLMSKAWQ"
     gene            complement(218..856)
                     /locus_tag="VIV63_05670"
     CDS             complement(218..856)
                     /locus_tag="VIV63_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124117.1"
                     /translation="MPGTVLKADVFGRVRRDGNTVIREAGCARPWARALAMHLLRREH
                     RALSRLALGTGIDGIPRILDFSATQLTRSWIDGSPMQIAQPRDPAYFRAALRLLRRLH
                     AAKVIHNDLAKETNWLVTPDGRPALVDFQLAMTLTKRGTLARALGHDDLRHLLKHKRS
                     YLPEKLTAREKRILARHSLVSRIWMASGKKVYLFVTRRILRWRDREGAGDRQ"
     gene            complement(860..2797)
                     /locus_tag="VIV63_05675"
     CDS             complement(860..2797)
                     /locus_tag="VIV63_05675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6124118.1"
                     /translation="MELPEQVPGAEASQFRLLGTRRFLPFFLTQFLGAFNDNLFRNAV
                     VVSITFGAAAASDAGVLANVSQGLFILPFFLFSALSGQLADKYEKSRLIRQTRFAEVV
                     LMCCGAVALYVGQISILLGVIFLLGVLATIFGPLKYSLMPQHLRQSELVGGNALVDAG
                     TFIAILVGTIAGGLLAPTASEGTAPTGGVDAHLAAAITMVVVAVVMYLCSRAIPRAEA
                     TDPTLKINFNPITSTFEVIRFAAKTRAIFLSLLGISWFWLVGALILAQLPAYAKDVIG
                     GDKTVYTLLLAAFSIGTALGSLACERLSGHKVEIGLVPLGSIGMTVCLLDLYFEQPGV
                     HEAGAAVVSWTQFLAAGGWDVALDCALIGLFGGLFIVPLYALILQRSAESHRARIIAC
                     NNIMNAGFMVIAALLAIVWLEVLDFTIPQLFILAAVLNAIVATYIYTLVPEFLMRFLS
                     WVFVNIMYRIKVRGLEHIPETGPVLIVCNHVSFMDPLVIGGSVRRPVRFVMDHNIFKI
                     PVMSFIFRTARAIPIAPAHEDPQALQKAFDQIDAELADGEIVCIFPEGKLTKHGELNE
                     FKKGVEKILERRPVPVVPMALRGLYGSFFSRKGGKAPMTRPPQRFWSRIEVVVTAPVP
                     GDDASATSLQRIVGGLRGEWQ"
     gene            2896..3204
                     /gene="yecR"
                     /locus_tag="VIV63_05680"
     CDS             2896..3204
                     /gene="yecR"
                     /locus_tag="VIV63_05680"
                     /inference="COORDINATES: protein motif:HMM:NF025358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HEY6124119.1"
                     /translation="MNRLLILASFALLAACTTYKLWNEAGSDQDARTVDLSYEYRKYE
                     SPQLDERAGVELARERCKDWGYPSARRKAEARECIDGTEDNCSKWRVTRQYLCTAPEK
                     "
     gene            complement(3219..3674)
                     /locus_tag="VIV63_05685"
     CDS             complement(3219..3674)
                     /locus_tag="VIV63_05685"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY6124120.1"
                     /translation="MKTTGVKTLPLLLCALLGGGILTTSCTTPAEPVNQAATQPLLNT
                     PWRLTQLGGEVIDNPPGNAAVNFLLQPSSTNLVGFSGCNRMFGRYALEGSSLKFDGLG
                     GTRMFCEATMPLEQRFLAMFAQVARWEITGSTLRLLDANGKSAATFATP"
     gene            complement(3686..4264)
                     /locus_tag="VIV63_05690"
     CDS             complement(3686..4264)
                     /locus_tag="VIV63_05690"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY6124121.1"
                     /translation="MTESPSTPRLRATFAIYLALIAATAVIGPQGIPKAWHYLTGLLG
                     FACVAMACLGRIWCSLFIAGHKDEVLVTTGPYAACRHPLYSFSVLGALGLGLTSRSAL
                     LCIVVVLLITALVVYAASCEEQFLADAFPDEFKAYLAATPNKWLPVWRDSPLPAVLDV
                     RPAVFWKAFLDAGSFLLLWILVALAAEYRIWV"
     gene            4346..4981
                     /gene="msrA"
                     /locus_tag="VIV63_05695"
     CDS             4346..4981
                     /gene="msrA"
                     /locus_tag="VIV63_05695"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY6124122.1"
                     /translation="MALLSKKTSIPAESDALPGRNTPLPLSGKHFVNGSSIKQPAAGG
                     LEEAVFGLGCFWGAERKFWQIPGVVTTAVGYAGGYTPNATYEEVCSGRTGHTEVVRVI
                     YDPNKVSYDTLLKAFWESHDPTQGMRQGGDVGTQYRSAIYTTTDAQLKAAEASKRSYQ
                     DALTQAHRATITTEIKDAPEFYYAEEYHQQYLAKNPNGYCGLGGCGVKYGA"
     gene            4995..5726
                     /locus_tag="VIV63_05700"
     CDS             4995..5726
                     /locus_tag="VIV63_05700"
                     /inference="COORDINATES: protein motif:HMM:NF044878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124123.1"
                     /translation="MDSDIKVQCDTHGETSAAFVCQHLLDGTELGFNVGVDPETPDEI
                     CPDAWCDGCEGRFAEVGEWTDELYAQADIKLVCTSCYSDIRERNWIQDDEAFDDLLQE
                     SIDHLNAKQDTLIPEFKLDKHARWDWHQETAQLVFSNDGKPAVICDIIFVGSISRTGD
                     TWLWSWANESLDEIVKTRMREVRAYGEEQRFEKLAGAFWSGTPEDGWHMTAIAAQLLN
                     AIGAYRTPDEKGFTYLVITRAAWAQ"
     gene            complement(5701..6444)
                     /locus_tag="VIV63_05705"
     CDS             complement(5701..6444)
                     /locus_tag="VIV63_05705"
                     /inference="COORDINATES: protein motif:HMM:NF025175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HEY6124124.1"
                     /translation="MPFTISHAAAVLPLRKLRLPLAAMMIGSMSPDFAYFVPGQTERV
                     ETHSIPGVFWFCWPVSLALWLLFVRVLEQPTTALLPESWRSRFPPSNRELTFATWAAA
                     SAAVVIGAMTHLLWDSFTHRGTGAVQLIPALASVAFHFDGWRIRWFVVLQHLSTIFGF
                     LILMIWAWRQPSGRYPRPAAPTFSNATRIRCAAIIVATSLGLAITGYVLNSDVWFTRR
                     LFHFAIGGMTGFAVAWLAIALITAPRLRA"
     gene            6708..7223
                     /locus_tag="VIV63_05710"
     CDS             6708..7223
                     /locus_tag="VIV63_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124125.1"
                     /translation="MRYFGAWAFAPLNEPVYRLALFIIPSSFYFFARWATQPSERFRP
                     IYLLHLLPVVLLFVVPLKVALPILLLSGIGYLLWVAWFIFGLRSQFKQFLFEFLCFTV
                     LWSTAMAMLAYGLLIPFMDDAFFHLFSANGIGLCLAIILLVLIVNAATASSGEATHSK
                     GRERSHHPPRR"
     gene            7518..8414
                     /locus_tag="VIV63_05715"
     CDS             7518..8414
                     /locus_tag="VIV63_05715"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY6124126.1"
                     /translation="MSKLRGVLALTIALLGAFAVPAVAQSKKILFLAGPRDHGAPGRH
                     EYERDLRTLATSFENASNLSGVTTQVILGRLPRDLAAVQDAAVIVIDSSSDRAENEVH
                     PLFPPNPTTNGRGYDEDTTAYLKALDERIRQNKIGIVILHYANWAENWRAREYHLSWT
                     GGLWVQMASRNPQDDWSMTLENKKHPILRGVKPWKYRDEVFCRFLLPNDQRRTDLVLA
                     SPKVDKFGIGPQIASWAYQRDDGGRGFVFGGLDFRDNLALDNYRRFLMNGIAWAGHIE
                     IPRGGIVSPTPDVSDVTPRKAQ"
     gene            8415..>8678
                     /locus_tag="VIV63_05720"
     CDS             8415..>8678
                     /locus_tag="VIV63_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124127.1"
                     /translation="MLVLRLMGVVIAFAACSAAAGAEWPDYGGTPDQSKYAVTRDITK
                     KNVSQLEVAWIYPTSDERAYQFNPVIVDNVMYVLAKNSSLVAID"
BASE COUNT         1704 a         2708 c         2780 g         1486 t
ORIGIN      
        1 ccgttcgtgg acctgctgtt gcccgtggac ttgggggcca cgcgcggtgt cgtgctctgg
       61 gtcaagttca tcatcgagct ttgctatttc atcttcgcga ttcacacctt cggaaagctg
      121 ccgctgtgga ataccgtcct gcggtgcatc ctggtgctgg gcgccgggaa cgcattgctg
      181 atcttcctca tgagcaaggc ctggcagtga tctgccgtca ctgccggtcg ccggcgcctt
      241 cgcggtcgcg ccagcgcagg atccggcgcg tgacgaatag atagaccttc ttgccactgg
      301 ccatccagat ccgcgacacc agcgaatgtc tggcgaggat ccgcttctcg cgcgcggtga
      361 gcttttcggg aagatagctg cgcttgtgct tgagcaggtg gcgcaggtcg tcgtggccca
      421 gcgcgcgggc gagcgtgccg cgtttcgtca acgtcattgc gagctggaaa tcgacgaggg
      481 cggggcggcc atcgggcgtc accagccagt tggtttcctt ggcgagatcg ttgtggatga
      541 ccttggccgc gtgcagccgg cgcagcaggc gaagcgcggc gcgaaagtag gcggggtcgc
      601 ggggctgcgc gatctgcatg ggcgacccgt cgatccacga acgcgtgagc tgcgtcgcgc
      661 tgaagtcgag gatgcgcgga atgccgtcga tgccggtgcc cagcgcgaga cgcgacagcg
      721 cgcgatgttc gcggcgcagg agatgcatcg ccagcgcgcg cgcccagggg cgcgcgcagc
      781 cggcttcacg gatgacggtg ttgccgtcgc ggcgcacgcg gccgaacaca tcggccttca
      841 aaacggtgcc aggcaccatt tactgccact cgccgcgcag gccgccgacg atacgctgca
      901 ggctcgtggc gctggcgtcg tcgcccggaa cgggcgcggt gaccacgact tcgatgcgcg
      961 accagaaacg ttgcggcgga cgcgtcatcg gcgccttgcc acccttgcgg ctgaagaagc
     1021 tgccgtacag cccgcgcagc gccatcggca ccacgggcac gggccggcgc tcgagaatct
     1081 tttcgacgcc tttcttgaat tcgttgagct caccatgttt ggtgagtttg ccctcgggga
     1141 agatgcagac gatctcgcca tcggccagct cggcgtcgat ctggtcgaat gccttctgca
     1201 acgcctgcgg atcttcgtgt gccggcgcaa tcggaatggc gcgggcggtg cggaagatga
     1261 agctcatgac cggaatcttg aagatgttgt ggtccatgac gaaacgcact ggccggcgca
     1321 cgctgccgcc gatcaccagc gggtccatga aactcacgtg gttgcagacg atgagcacgg
     1381 ggccggtctc cggaatgtgc tcgagtccac gcaccttgat gcggtacatg atgttgacga
     1441 acacccagga caggaaccgc atcaggaatt cgggcaccag cgtgtagata tacgtggcca
     1501 cgatggcgtt gagcaccgcc gccaggatga agagctgcgg gatggtgaag tccagcactt
     1561 cgagccagac gatggctaac aacgccgcga tcaccatgaa accggcgttc atgatgttgt
     1621 tgcacgcgat gatgcgcgcg cgatgactct cggcgctgcg ctgcaggatc agcgcgtaga
     1681 gcggcacgat gaacaggcca ccgaacaagc cgatcagcgc gcagtcgagt gcgacatccc
     1741 agccgcccgc ggccagaaat tgcgtccacg agaccacggc ggcgcctgct tcgtgcacgc
     1801 cgggctgttc gaagtacaga tcgagcaggc acaccgtcat cccgatggac ccgagcggca
     1861 ccaggccgat ctccaccttg tgaccggaca ggcgctcgca ggccagcgag cccagcgccg
     1921 tgccaatcga gaaagccgcg agcagcagcg tgtacacggt cttgtcgccg ccgatcacat
     1981 ccttcgcata ggcaggcaac tgcgccagga tcagtgcacc caccagccag aaccacgaga
     2041 tgcctaacaa cgaaaggaag atcgcacgag tcttcgccgc gaaccggatc acttcgaatg
     2101 tcgaggtgat cgggttgaag ttgatcttga gtgtgggatc ggtggcttcc gcgcgcggaa
     2161 tagcgcgcga gcacaggtac atgaccaccg cgaccaccac catggtaatc gccgccgcga
     2221 gatgcgcatc gactccgccc gtcggcgcag tgccttcgct tgcggtaggc gcgagcaggc
     2281 cgccggcaat cgtgcccacc aggatggcga tgaaggtgcc ggcgtccacg agggcgttgc
     2341 cgccgacgag ctcgctctgg cgcagatgct gcggcatcag cgaatacttg agcgggccga
     2401 agatggtggc cagcacgcct aacaggaaga tcacgccgag caggatcgaa atctgtccca
     2461 cgtacagcgc caccgcgccg cagcacatga gcacgacttc ggcgaagcgc gtctgccgaa
     2521 tcagccggct cttctcgtat ttgtccgcga gctggccgga caatgccgaa aacaggaaga
     2581 acggcaggat gaacaggccc tgcgacacgt tggccagcac gccggcatcg ctggccgcgg
     2641 cagcgccgaa cgtgatggac acgaccaccg cgttgcgaaa caggttgtcg ttgaaggcgc
     2701 cgagaaactg ggtgaggaaa aacggcagga agcggcgcgt tcccagcagc ctgaactgcg
     2761 acgcttccgc acccggaacc tgttcgggga gttccatcag gcgcgtgccg gccggcgact
     2821 atgcgttgac atccggcggc atatatacca gaaccccgct gtcggtccgg tactgaaaaa
     2881 cgctacagtg agtctatgaa ccgtctattg atcctggcca gtttcgcgct gcttgcggcc
     2941 tgcaccacct acaagctgtg gaatgaggcc ggcagcgacc aggacgcgcg tacggtcgac
     3001 ttgtcatatg agtaccgcaa gtacgaaagc ccgcaattgg acgagcgcgc cggcgtggag
     3061 ttggcgcgcg agcgctgcaa ggactggggc tacccgtcgg cgcggcgcaa ggccgaagcc
     3121 cgcgagtgta tcgacggcac cgaagacaat tgcagcaagt ggcgtgtgac gcggcagtac
     3181 ctctgcacgg caccagagaa gtaacccggc ccagcttgtt agggcgtggc gaacgtcgcc
     3241 gctgatttcc cgttcgcgtc gagcaggcgc agcgtcgagc cggtgatttc ccagcgagcc
     3301 acttgcgcga acatcgccag gaatcgctgc tccagcggca tggtcgcctc gcagaacatc
     3361 ctcgtgccgc cgagcccgtc gaacttcagg gaactgccct cgagcgcata acgcccgaac
     3421 atgcggttgc acccggagaa gccgacgaga ttcgtgctcg acggctgcaa caggaaattc
     3481 accgctgcgt tgccgggcgg attgtcgatg acttccccgc cgagttgcgt gagccgccag
     3541 ggggtgttga gcagcggctg cgtggcggcc tggttcacgg gctcagccgg ggtggtgcac
     3601 gaggtagtta ggatgccgcc gccgagcaag gcgcacagca gcagggggag ggttttcacg
     3661 ccggtggttt tcatggcgca aaatcctaca cccagatgcg gtactccgct gccagggcga
     3721 ccagtatcca caacagcagg aacgacccgg catccaggaa cgccttccag aacacggccg
     3781 ggcgcacgtc cagcaccgcc ggcaacggtg agtcgcgcca cacgggcagc catttattgg
     3841 gggtggcggc aaggtaggcc ttgaattcgt ccggaaaggc gtcggcaagg aactgctctt
     3901 cgcacgaagc cgcgtagacc acgagcgcgg taatcagcag caccaccacg atgcacaaca
     3961 acgcggaccg gctggtgagc ccgagcccca gcgcgccgag cacggagaat gaatagagcg
     4021 ggtggcggca cgccgcatag gggccggtgg tgaccagcac ttcatccttg tggccggcga
     4081 tgaacagcga gcaccagatc cggccgaggc aggccatcgc cacgcacgcg aaacccagga
     4141 ggccggtcag gtaatgccag gccttgggga tgccctgcgg gccaatgacc gcggtcgccg
     4201 cgatcagggc gaggtagatc gcgaacgtgg cgcgcaggcg cggtgtgctg ggggattctg
     4261 tcatcggcgg cgcagtctag cagttgagaa cgcgcaccca agcccccaca cttgcgcacc
     4321 tctcccgcga taccaggagc cgcgcatggc actcctttcc aagaagacat ccatccccgc
     4381 cgaaagcgac gcactgccgg gccgtaacac gccgctgccg ctgtcgggca agcacttcgt
     4441 gaacggcagc agcatcaagc agcccgccgc gggcggcctc gaagaagccg tgttcgggct
     4501 cggttgcttc tggggcgcgg aacgcaagtt ctggcagatc ccgggcgtgg tcaccaccgc
     4561 ggtcggatat gcagggggtt acacgcccaa tgccacctat gaagaggtgt gttcgggccg
     4621 caccggccac accgaagtcg tgcgcgtgat ctacgaccct aacaaggtca gctacgacac
     4681 actgctcaag gcattctggg aatcacacga tccgacccag ggcatgcgcc agggcggaga
     4741 cgtgggcaca cagtatcgct cggctatcta caccaccacg gatgcgcagc tcaaagccgc
     4801 ggaggccagc aagcgctcct accaggacgc cttgacgcag gcacatcgcg cgaccatcac
     4861 caccgagatc aaggacgcgc cggagttcta ctacgccgag gaatatcacc agcagtacct
     4921 cgccaagaac ccgaacggtt attgcgggct cggcgggtgt ggagtgaagt acggagcctg
     4981 acagcaccgc ctcgatggat agcgacatca aggtccagtg cgacactcat ggcgaaacca
     5041 gtgccgcgtt cgtctgccag catctgctcg acggcacgga actgggtttc aacgtcggcg
     5101 tggatcccga aacgcccgat gaaatctgcc cggacgcgtg gtgcgacggc tgcgagggcc
     5161 gtttcgcgga agtcggcgag tggacggatg aactgtacgc acaggcggac atcaagctgg
     5221 tgtgcacgtc gtgctactcg gacatccgcg agcgcaactg gatccaggac gacgaagcct
     5281 tcgacgatct gctgcaggag tcgatcgacc acctgaacgc caagcaggac acgctgatcc
     5341 cggagttcaa gctggacaaa cacgcgcgtt gggactggca tcaggaaacg gcgcagctcg
     5401 tgttctccaa cgacggcaag cccgcggtga tttgcgacat catcttcgtc ggcagcatct
     5461 ccaggaccgg cgacacctgg ctctggtcct gggccaatga atcgctggat gaaatcgtca
     5521 agacgcgcat gcgcgaggtg cgcgcctatg gcgaagaaca gcgcttcgag aaactggccg
     5581 gggcgttctg gtcgggaacg ccggaagacg gctggcacat gacggctatc gcggcgcagc
     5641 tgctgaacgc catcggcgct taccggacgc cggatgaaaa aggcttcacc tacctggtga
     5701 ttacgcgcgc agcctgggcg cagtaatcag cgcgattgcc agccacgcaa cggcaaagcc
     5761 cgtcatgccg ccgatcgcga agtgaaacag gcggcgcgtg aaccagacat cggagttgag
     5821 cacatacccc gtgatcgcga gtccgagcga cgtggccacg atgatggcgg cgcaacgtat
     5881 gcgtgtcgcg ttggaaaaag taggcgccgc gggtcgggga tagcggccgg atggttgcct
     5941 ccatgcccag atcatgagga tcaggaaacc gaagatggtg ctcaggtgct gcagcaccac
     6001 gaaccagcgg atgcgccagc catcgaaatg aaacgcgacc gaggccagtg cgggaatcaa
     6061 ttgaaccgcg ccggtgccgc gatgcgtgaa cgaatcccac aagagatgcg tcatggcgcc
     6121 gatgacgacc gcggccgatg ccgccgccca tgtcgcgaac gtcaattccc gattggaagg
     6181 cgggaagcgt gaacgccagc tctccggcag cagcgcagtc gtaggttgtt cgagcacgcg
     6241 cacgaacagc agccagagcg cgaggctcac cggccagcag aaccagaaca cgcccgggat
     6301 gctatgcgtc tcgacgcgct cggtttgacc cggcacgaaa tacgcgaaat cgggtgacat
     6361 gctgccgatc atcatcgcgg cgagcggcag tcggagtttg cgcagcggaa gcactgccgc
     6421 tgcgtgggaa atggtaaacg gcacgcgcgc gatcctatca gccgcgcctg cgcgaaatgg
     6481 tgaccaattg tggcgatcag cccggagttg cgggccgggc aggtgcgcgg ttagtttcgt
     6541 cgcccatgat caagctcagc atcgcaattg ccggattttc cgtcgtcgct tccgtgttgc
     6601 tcttcttcat ctacgctttc ttccggcgcg gccccggtag atccgcctac tccatatttt
     6661 tctgcgctgt gttactggcg gcgattgtca cggtccaggt ttttcacatg cggtacttcg
     6721 gcgcgtgggc gtttgcgcct ctcaacgagc ccgtttacag actcgcactg ttcatcattc
     6781 cttcatcgtt ctatttcttc gcccgatggg cgacgcagcc gtcggaacgc ttccgtccca
     6841 tctacctgct gcacctgctg ccagtcgtgc tgctcttcgt cgttccgctg aaagtcgcgc
     6901 tgcccatact gctgctgtcc ggcatcggtt acctgctgtg ggtggcctgg ttcatattcg
     6961 gcctgcggtc ccagttcaaa caattcctct tcgagttcct gtgtttcacc gtgttgtggt
     7021 cgaccgcgat ggcgatgctc gcctacggcc tgctcattcc attcatggat gacgctttct
     7081 tccacctctt cagcgcgaac ggcatcggcc tgtgccttgc gatcatcctg ctggtgctca
     7141 tcgtgaatgc agccacggca tcatcgggcg aagccactca tagtaaaggg cgtgaacgct
     7201 cgcaccaccc accgcgccgg tgagacgcaa ccgctcgcgc gcagtgactg cgcgcgagtg
     7261 gttgacgagt gaaccgaatc agacggacgt cagttcggtg ttggcgcgag acccttcctg
     7321 cccattgctg gacccccctc agtcagagtg atgacggcaa cttcgtctgt tgctcccgct
     7381 gctctcgccc gttccgcgtt cgggcatggt ttagtaacac aagccatcga aactctgcaa
     7441 tcgaccgagg tcgaaggtcg tacgttggcc cggttgaatg cagaatgggc cacaaatcca
     7501 aggttgcagg ggttttcgtg tccaagttac ggggcgtttt ggcgctgacg atcgccttgc
     7561 taggagcgtt tgccgttccg gcggtcgcgc agagcaagaa gatcctgttt ctggccggac
     7621 cgcgcgatca cggcgcaccg ggccgtcatg aatacgaacg cgacctgcgc acgctggcga
     7681 catcattcga gaacgcgtcg aacctgagcg gcgtcaccac gcaagtcatc ctgggccgac
     7741 taccccgcga tctcgccgcc gtacaggacg cagccgtcat cgtgatcgac agcagttcgg
     7801 atcgtgcgga gaacgaagtc catcctttgt ttccaccgaa tcccacgacc aacggccgcg
     7861 gctacgacga agacaccacg gcctatctaa aggcgctgga cgagcgcatc cggcagaaca
     7921 agatcggcat cgtcatcctg cactacgcga actgggccga gaactggcgc gcgcgcgaat
     7981 accacctgag ctggaccggc ggcctgtggg tgcagatggc gtccaggaat ccgcaggacg
     8041 actggtcgat gacgctcgag aacaaaaagc atccgatcct gcgcggcgtg aagccgtgga
     8101 aatatcgcga cgaggtcttc tgccggttcc tgctgcccaa cgaccagcgg cgcacggatc
     8161 tggtgttggc ctcgcccaag gtggacaagt tcggcatcgg tccgcagatc gcctcgtggg
     8221 cctaccagcg tgacgacggt ggccgcggct tcgtgttcgg cggcctggat tttcgcgaca
     8281 acctcgcgct cgacaactac cgccgctttc tcatgaacgg cattgcctgg gccgggcata
     8341 tcgaaatccc gcgcggcggc atcgtctcgc cgacgccgga cgtcagcgac gtcactccgc
     8401 gcaaggcgca ataggtgctc gtcttgcgtt tgatgggcgt ggtgatcgcg ttcgcggcct
     8461 gttcggcggc cgccggcgcc gagtggccgg attacggcgg cacgccggat caatccaaat
     8521 acgcggttac ccgggacatc acgaagaaga atgtctcgca gctcgaagtc gcctggatct
     8581 atccgacgag cgatgagcgc gcttaccagt tcaatccggt catcgtcgac aacgtcatgt
     8641 acgtgctcgc gaagaacagc tcgctggtgg ccatcgat
//
LOCUS       DATDEU010000232         6690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1790361, whole genome shotgun sequence.
ACCESSION   DATDEU010000232 DATDEU010000000
VERSION     DATDEU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790361"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VIV63_05725"
     CDS             <1..861
                     /locus_tag="VIV63_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6124128.1"
                     /translation="LSFFIIRLAPGGPFDQPQALPPEIRANLDAAYGFDKPLISQYGH
                     YLAGVVQGDLGPSLRYKDFNVTELIARGLPLSLTIGLAAALLAFFAGVLLGAIAAWRA
                     NSFADHAVMGFSMLGVVLPGFVVGPLLALIFGIYWPVFRVAGYEPGVVSFLVLPVVTL
                     ALPVAAYVARLMRNSLEEVLRANYIRTARAKGLGASAIFLRHALRPALIPVVSYLGPA
                     VAFVVTGSLVVESVFGLPGSGRFLVQGAVDRDYPLVMGMILVYGVLTLTCNLIADLLY
                     AWLDPRVRLA"
     gene            858..1679
                     /locus_tag="VIV63_05730"
     CDS             858..1679
                     /locus_tag="VIV63_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6124129.1"
                     /translation="MSVINGKGTRTALVVLALLAALALLAPWITPYSHEHIDWEHQAA
                     PPQLEAAHWLGTDRLGRDLFARSMQALRVSLLIGVLAALVSVCIGVAWGATAGYVGGR
                     VDAFMMRFVDIVYALPYVFIVIILSTMFERGSIAALFIAIGAVGWLTMARIVRGQTLG
                     LRRREFIDAAIASGVTTPAILWRHIVPNVIGPVIAYATLTIPQMILFESFLSFLGLGV
                     QEPFASLGSLINEGAQEMESSAWMLLVPATLLTVLLIAFNLLGNGLRAALESRDE"
     gene            1672..3483
                     /locus_tag="VIV63_05735"
     CDS             1672..3483
                     /locus_tag="VIV63_05735"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6124130.1"
                     /translation="MNDGALLIVEDLAVSYGATPAVESVSLEIAPGRCLGVIGESGAG
                     KTQAFLAMMGLLPAHARVAGRARLEGEDLLGPAAARLRGRRVAMIFQDPMTSLTPHMR
                     IGDQIAEPLVTHQGFRWQDARAKAAVLLEQVRMNDVPRRLRQYPHELSGGMRQRAMIA
                     MALACDPKLLIADEPTTALDVSVQAQILALLRQLVAERGMSLAVVTHDMGVIAALADD
                     VVVMRDGRIVERGPVAQILANPAHEYTRALLAATPKLETPGAGSVQDGEPDAAPITPL
                     SVHDLRVHHRLRGGWLRPKVTLAAVDGVSLSIQAGEALGIVGESGCGKSTLSRALLRL
                     GPVTGGEIVWLGRAIEHLAGEELRTLRTGMQIVFQDPFASLDPTMRVHDIVAEPLRAL
                     RPELDAAQRSARVVAMLTSVGLGPEFADRRSRELSGGQCQRVAIARAMVLEPQLLVCD
                     EAVSALDVSIQAQLLELFAAIKREHGTSILFVSHNLAVVRQLCERVLVMYLGRVVEEG
                     PAEEVFLRPRHPYTRMLLDSVPLLDPARERARLSALAVQGETPSAVDRPSGCHFHTRC
                     PHALPTCARDSPEPEVVGKSHQVACLRWREIAGDIVK"
     gene            3562..4836
                     /locus_tag="VIV63_05740"
     CDS             3562..4836
                     /locus_tag="VIV63_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6124131.1"
                     /translation="MSQETHIRPSEGLRHVKYEIRGRLARRAQELERQGYEIVPLNIG
                     NPGAFGFRTPETMRLAMIENMAVAEGYCHQKGIFPAREAVVMQQQSRGVSGISADEVF
                     IGNGVSELIDLCLRALLNPGDEVLIPSPDYPLWSAAVTLNGGKAVYYPCRPENDFVPD
                     PEEIAALVTRKTRAIVVINPNNPTGAVYPRAVCEGIARLAEANKLVVFSDEIYDQMTY
                     EGAESIPMATLVRDTVCCTMSGLSKIYRACGYRVGWAAFSGNLRGAGDYLSALELLSS
                     LRLCSNVPAQWAVQTALGGYQSVKELLTPGGRLFESRRAILEATAASRFLSLAPPKGS
                     MYAFVRVKTDELPDFDDQTFALDLLEQKHVLLAPGTSFNVPYRDHFRTTLLPDAALLK
                     KVFVRIDELLSTYASAPAGDKARVVHAESRFK"
     gene            4850..>6690
                     /locus_tag="VIV63_05745"
     CDS             4850..>6690
                     /locus_tag="VIV63_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124132.1"
                     /translation="MNSKRLPAPLVDGLETGATLVVPSPQRQAAVRAAWVLAQRDADR
                     TWWATPCILTFGQFAEGVLQEQWARANLPDRLLPPGAEWACLRDARRDAGGPAEARAL
                     LNSVRTLSDWRIAASPRLLGVSPESELLIDALATLERLSTDANRKPMRAWLDDLQRPP
                     ETLVAAGIANLPSAQRETLLRLGAFAPPALSAAVAEDLASVSMATAQNDEHELELIAG
                     WCRSELERDPGQRLLIVDAKLRQRRGLYERLLSQTLAPSEWISPAARTASSVFAIEGG
                     RPLAEFPLIAHALLCLRLLTGRLRFDEVVRLLRLPFLDSGDVMAGAAIEACLRDGRKL
                     EFHGEDLAAFLERAGGAAPAAFAARLQQALVTLAGQRRTPAEWAPRLLSALRQLGWHG
                     TRALRSDEQQTVNRWHALLDEYSALGPWLRQSSAAEAVATLADLAGERNFDAASVEAP
                     VTLTESHDDPIVRYDGIWGAGLDAAQWPPAPRPDVFIPLRLQVAAGVPWASAAAQTRA
                     ARQSLAAWRSSTDRLVCSWARLEGDAHRTPSPLLIHLRERNDYAPPSRIVPLAAALHR
                     PELETLEDGQGVKIDTSRPVAGGVKPLALQAECGFRAYGEMRLGADVL"
BASE COUNT         1017 a         2222 c         2237 g         1214 t
ORIGIN      
        1 ttgtcgttct tcatcatccg cctcgccccc ggcggaccct tcgatcaacc gcaggcgttg
       61 ccgcccgaga tccgcgccaa tctcgatgcc gcgtacggtt tcgacaagcc gctgatttcg
      121 cagtacggac attacctggc cggagtggtg cagggcgatc tcggcccctc gctgcgttac
      181 aaggatttca acgtcaccga gctcatcgcg cgcggcctgc cgctcagcct aactatcggg
      241 ctcgcggcgg cgctgctggc gtttttcgcc ggcgtgctgc tcggtgcgat cgcggcctgg
      301 cgcgccaatt cctttgccga tcatgccgtc atgggtttct cgatgctggg cgtggtgctg
      361 ccggggttcg tcgtcggtcc gttgctcgca ctgattttcg gaatctactg gccggtcttt
      421 cgagtcgccg gctacgagcc cggggtggtg agttttctcg tgctgccggt cgtcacgctg
      481 gcgctgccgg tcgccgccta tgtcgcgcgc ctgatgcgca acagcctcga agaggtgctg
      541 cgggcgaact acatccgcac cgcgcgcgcc aagggattgg gcgccagcgc catcttcctg
      601 cggcacgcgt tgcgtccggc gctcatcccc gtcgtgagtt acctcgggcc cgcggtcgcg
      661 ttcgtggtca ccggatcgct ggtcgtcgaa tcggtgttcg gtttgcccgg ctccggccgc
      721 tttctcgtgc agggcgcggt cgatcgcgac tatccgctgg tcatgggaat gatcctggtc
      781 tacggcgtgc tgacgcttac gtgcaatctc atcgccgatc tgctgtacgc ctggctcgat
      841 ccgcgcgtgc gcctcgcatg agcgtcatca acggaaaggg cacacgcacc gcgctcgtcg
      901 tcctcgcgct gctcgcggcg ctggcactgc tcgcgccgtg gatcacgccg tacagtcatg
      961 aacacatcga ttgggaacac caggcggcgc caccgcagct cgaagccgcg cactggctgg
     1021 gcacggaccg cctgggccgc gacctgttcg cgcgcagcat gcaggcgctg cgcgtgtcgc
     1081 tgctgatcgg cgtgctcgcg gcgctggtca gcgtgtgtat cggcgtggcc tggggcgcga
     1141 ccgccggtta cgtgggcggg cgggtggatg cgttcatgat gcgcttcgtc gacatcgtct
     1201 acgcgttgcc ctacgtgttc atcgtcatca tcctgtcgac gatgttcgag cgcggcagca
     1261 tagcggcgtt gttcatcgcg atcggcgccg tcggctggct caccatggcg cgcatcgtgc
     1321 gcgggcagac gctgggattg cggcgccgcg agttcatcga tgccgccatt gcaagcggtg
     1381 tgacgacgcc tgcgatcctc tggcggcaca tcgtgcccaa tgtcatcggg cccgtgatcg
     1441 cctacgcgac gctcaccatc ccgcagatga tcctgttcga gagtttcctg agtttcctgg
     1501 ggctgggcgt gcaggagccg ttcgcgagtc tcggcagcct catcaacgag ggcgcgcagg
     1561 agatggaatc gtcggcgtgg atgttgctgg tgccggcgac gctgctgacc gtgctgctga
     1621 tcgcgttcaa cctgctcggc aatggattgc gcgccgcact cgagtcccgc gatgaatgac
     1681 ggcgcgttgc tgatcgtcga ggacctggcg gtgagttacg gcgccacacc cgccgtcgaa
     1741 tcggtgtcgc tcgagatcgc gccgggtcgc tgtctcggtg tcatcggcga atccggcgcc
     1801 ggcaagacgc aggcattcct cgcgatgatg ggtctgcttc ccgcgcatgc gcgagtcgcc
     1861 ggccgggcgc gtctcgaagg cgaggatctg ctcgggcccg cggcggccag gctgcgcggt
     1921 cgccgcgtgg cgatgatctt ccaggacccg atgacctcgc tgacgccgca catgcgcatc
     1981 ggcgatcaga tcgccgagcc gctggtcacg caccagggct tccgctggca ggatgcccgt
     2041 gcgaaagccg cggtgctgct cgagcaggtc cgcatgaacg acgtgccgcg ccgtctgcgc
     2101 cagtatccgc atgaactctc gggcggcatg cggcagcggg ccatgatcgc catggcgctc
     2161 gcctgcgatc cgaaactgct gatcgccgat gagccgacca cggcgctcga cgtgtcggtg
     2221 caggcgcaaa tcctcgcgct gcttcggcag ctcgttgccg aacgcggcat gtctctggcg
     2281 gtggtcacgc acgacatggg cgtcatcgcc gcgctcgccg acgacgtggt cgtgatgcgc
     2341 gatggccgaa tcgtcgaacg cggaccggtt gcgcaaatcc tggcgaatcc cgcccacgaa
     2401 tacacacgcg cgctgctcgc ggcaactccg aaactggaaa cgccgggagc ggggagtgtg
     2461 caagacggag aacctgacgc ggcgcccatc acccctctgt cggttcacga ccttcgtgtg
     2521 catcaccgcc tgcgcggtgg ctggttgcga ccgaaggtca cgctcgccgc cgtcgatggt
     2581 gtgtcgctga gcatccaggc gggcgaagca ctgggcatcg tcggcgagtc tggctgtggc
     2641 aagtcgacgc tcagccgcgc gctgctgcgg ctcggtccgg tcacgggcgg agagatcgtg
     2701 tggttaggcc gcgccatcga gcacctggcg ggtgaggaac tgcgcacttt acgcaccggc
     2761 atgcagatcg tgtttcagga tccttttgcg agcctggatc cgaccatgcg cgtgcacgac
     2821 atcgtcgccg aacccttgag agcgctgaga ccggagctgg acgccgcgca acgctcggcg
     2881 cgagtcgtgg ccatgctcac cagcgtcggt ctcggacctg aattcgcgga ccggcgcagc
     2941 cgcgagctct ccggcggcca atgccaacgc gtggcgatcg cccgtgcgat ggtcctcgaa
     3001 ccccaactac tggtgtgcga cgaggcggtc agcgcgctcg acgtgtcgat ccaggcgcag
     3061 ctgctcgagt tgttcgcggc gatcaagcgt gaacacggca ccagcatcct gttcgtgagc
     3121 cacaacctgg cggtagttcg ccagctctgc gagcgggtcc tggtgatgta cctgggccgt
     3181 gtcgtggaag aaggcccggc cgaggaggta ttcctgcgcc cccggcaccc gtacacgcgc
     3241 atgttgttag attcagtgcc gctgctcgat ccggcccgtg aacgcgcgcg cttgtcggcg
     3301 ctcgccgtgc agggcgaaac gccgtcggcc gtggatcgcc cgagcggttg ccacttccac
     3361 acgcgctgtc cgcacgcgct acccacctgc gcgcgcgaca gtccagagcc ggaagtagtc
     3421 ggaaaatcgc accaggtggc ctgcctgcgc tggcgcgaga ttgccggtga tatcgtgaaa
     3481 taggccggat ttccgcggcc ggatacgcaa ggacggcggg ttcgcgttat tctccggcat
     3541 cgtttttcaa gcggtagcgt catgtcccag gaaacccaca tccgtccgag cgaaggcctg
     3601 cgtcacgtca agtacgagat acgcggccgg ctcgctcgac gcgcccagga gctcgagcgc
     3661 cagggctacg agatcgtgcc gctgaacatc ggcaatcccg gcgctttcgg tttccgcacg
     3721 cccgagacca tgcgcctcgc catgatcgag aacatggcgg tggccgaggg ctattgccac
     3781 cagaagggca tcttcccggc ccgcgaagcc gtggtcatgc agcagcagtc gcgtggcgtg
     3841 tccggcatca gcgccgacga agtgttcatc ggcaacggcg tcagcgaact catcgacctg
     3901 tgcctgcgcg cgctgctcaa tccgggcgac gaagtgctca ttccgagccc tgactacccg
     3961 ttgtggtcgg cggcggtgac gttgaacggc ggcaaggcgg tttactaccc gtgccgtccc
     4021 gagaatgact tcgtacccga tcccgaagag atcgcggcgc tggtcacacg caagacgcgc
     4081 gcgatcgtcg tcatcaatcc caacaatccg accggcgccg tctatccacg tgcggtgtgc
     4141 gaaggcatcg ccagactcgc ggaagcgaac aagctcgtgg tcttcagcga cgaaatctat
     4201 gaccagatga cgtacgaggg cgccgagtcc atcccgatgg cgacgctggt gcgcgacacg
     4261 gtgtgctgca cgatgtcggg cctgtcgaag atctaccgcg cctgcggcta tcgcgtcggc
     4321 tgggccgcgt tctccggcaa cctgcgcggc gcgggcgact atctatccgc gctcgagctc
     4381 ctgtcgtcat tgcggttgtg ctcgaacgtt ccggcgcagt gggcggtgca gaccgcgctc
     4441 ggtggctacc agagcgtcaa ggaactgctg actccgggcg gccggctgtt cgagtcgcgt
     4501 cgcgcaatcc tcgaggccac cgcggccagc cggttcctgt cactcgcgcc gcccaaaggc
     4561 tccatgtatg ccttcgtgcg cgtgaagacg gacgaattgc ctgacttcga cgaccagact
     4621 ttcgcgctcg atctgctcga gcagaaacac gtgctgctgg cgccgggcac cagcttcaac
     4681 gtgccgtatc gcgatcattt ccggaccacg ctgctgccgg acgcggcgtt gctcaagaaa
     4741 gtcttcgtga gaatcgacga gttgctgtcg acgtacgcca gcgcgcctgc cggcgacaaa
     4801 gcgcgcgtgg tacacgcgga atctcgcttt aagtaacagt cgtaagttat tgaacagtaa
     4861 gagactaccg gctccacttg tcgatggcct ggagacgggc gcgacactcg ttgtcccgag
     4921 tccgcagagg caggcggcgg ttcgcgccgc atgggtactc gcgcagcgcg acgcggaccg
     4981 gacctggtgg gccacgccat gcatcctgac tttcggccag tttgccgaag gcgttcttca
     5041 ggagcagtgg gcgcgcgcca atctacccga ccgtttgctg ccgcccggcg ccgagtgggc
     5101 gtgcctgcgc gatgcgcgcc gcgacgcggg tggcccggcg gaagcacgag cgctgctcaa
     5161 ctcggtccgc acactctccg actggcgcat tgccgcctcg ccgcgactcc tcggagtttc
     5221 gccggaatcc gaattgctca tcgacgccct ggcgacgctc gagcgcctgt ccactgacgc
     5281 aaaccggaaa cccatgcgcg cgtggctcga cgacctccag cgaccgccgg aaaccctggt
     5341 ggccgccggc atcgccaatc tgccgtcggc gcagcgggag accttgcttc ggctgggcgc
     5401 gtttgcaccg cccgcgcttt ccgccgcggt cgccgaagac cttgcgtcgg tatcgatggc
     5461 cacggcgcaa aacgacgagc atgaactcga actcatcgcg gggtggtgca gatccgaact
     5521 cgagcgcgac cctggacaac ggctgctgat cgtcgatgcg aagctgcgcc agcggcgcgg
     5581 tctctatgag cggttgctgt cgcaaaccct ggcgccgtcc gaatggattt caccggcggc
     5641 gcgaacggca tcatcggtgt tcgcgatcga aggcgggcgg ccgctcgccg aattcccgct
     5701 catcgctcat gcactgttgt gtctgcggct tctgaccgga cgcctgcggt tcgacgaagt
     5761 cgtgcgcctg cttcgccttc cgtttctcga cagcggcgac gtcatggctg gcgcggcgat
     5821 cgaggcctgc cttcgcgacg gccgaaagct cgaattccac ggcgaagatc tcgcggcgtt
     5881 cctcgaacgc gccggcggag cggctccagc cgcgtttgcc gcgcgtctgc aacaggcgtt
     5941 ggtcacgctc gccggccagc ggcgcacgcc ggcagagtgg gcgccgcggc tgctctcggc
     6001 gttgcggcaa ctcggctggc atggcacgcg tgcgttgcgc agcgacgaac aacagaccgt
     6061 gaaccgctgg cacgccttgc tcgacgagta ctccgcgctc ggcccgtggt tgcgacagtc
     6121 ttcggccgcc gaggccgtgg ccacgctcgc ggatctggcg ggcgaacgga acttcgatgc
     6181 ggccagcgtc gaagcgcccg tgacgctcac cgaatcgcat gacgatccga tcgtgcgcta
     6241 cgacggtatc tggggtgcag gactcgatgc cgcgcaatgg ccaccggcgc cccgcccgga
     6301 cgtcttcatt ccgttgcggt tgcaggtggc ggctggcgtg ccctgggcga gcgcggcggc
     6361 gcagacgcgc gcggcgaggc agtcactggc cgcctggcgt tcaagtaccg accggctggt
     6421 ctgttcgtgg gcgcggctcg aaggtgatgc gcatcggacg cccagtcctt tgctcatcca
     6481 tctgcgcgaa cgcaacgact acgcgccgcc gtcgcgcatc gtgccgctcg ccgctgcgct
     6541 gcatcggccg gaactcgaga cgctcgaaga cgggcagggc gtgaagatcg acacatcgcg
     6601 gcccgttgcc ggtggcgtca aaccactcgc gttgcaggcg gaatgtggat ttcgcgccta
     6661 tggggagatg cgtctcggcg ccgatgtact
//
LOCUS       DATDEU010000233        11427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_478317, whole genome shotgun sequence.
ACCESSION   DATDEU010000233 DATDEU010000000
VERSION     DATDEU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478317"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VIV63_05750"
     CDS             <1..526
                     /locus_tag="VIV63_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6124133.1"
                     /translation="QDSGVSAASLELEITESAMMLDPARALRVIQEIDELGVFISIDD
                     FGTGYSSLGYLRDLPVHSLKLDKSFVMGMRNNSDDRVIVESTAQMAHALKLELVAEGV
                     ETEWDAQFLAAAGYDYAQGFRYTRALPDDQCLAWIVEFNATAMLTLGDADVTATLRSR
                     AARDDEDDFESRTA"
     gene            complement(523..1635)
                     /locus_tag="VIV63_05755"
     CDS             complement(523..1635)
                     /locus_tag="VIV63_05755"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HEY6124134.1"
                     /translation="MKNKLARVAACGLALAVGCVNSAQADESGRFYGMLRSRDLTPFG
                     FLRLDMRPAHAVNIEKHTFAFEAELGYQNTWALSENVEKYLTSLESSGRREIGPAEIA
                     AIRDLPGENYLLDLESATLDLAVHYKISSQWTAYAFATAVSYQGGFLDSTIEGFHDLV
                     GLSSFGRPAIARNGTHVIFDLKSSQLVMNEPPSSSGFLDPTFGLRYSGFSLPGRWQMS
                     VETAVKIPISGARPLLSTGNTDYGMQASVRRLGERNALHIDFAAVYYAGEDLPAPNES
                     QIIPTIVVGWERQMSARTNLNLQAYASTSVYKPAQTDLDELLSDKFQLSLGVRHRFDC
                     CLVSFGITENLQNFNNTPDIGFQMGFAWVPRLKPQT"
     gene            1890..4784
                     /locus_tag="VIV63_05760"
     CDS             1890..4784
                     /locus_tag="VIV63_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6124135.1"
                     /translation="MKKQSKNRPALTALLPSLLVAATASAQDAPADPTMQEVVVTGSR
                     IIQSSANSQQPISIIDRATIDKTGLASIGELLQQVTAGGKALNAKFNSSGNFGYPADG
                     GGIGAGSAQVDLRNLGSKRVLVLVDGIRWVNESSASGVSGAADLNTIPMSIIERVEVL
                     EDGASAIYGSDAIAGVVNIITRKEFEGVELNGYFGEYELGGSTTDASFTLGGGGERFR
                     GLFVASYYDQEGIDSGEWDQSSFPQPNAGLSAGSSATPQGRYGFCDPARPAASYGSCS
                     ASQEFFYDVTLNNGTTTPVWNPADPSAGSYHDFSGADRFNYAPYNLLLTPSERKSMFA
                     RMSFDVSDMVTLNVKGLFNNRRSTNRAAPEPIFVGPYAGTGGLADTISISALNPFNPF
                     GIDLDASSNFGWVTRRPVEVGPRLFEQDVDTWYFNLGLEGKFGSDNRFHWDLNFVSSE
                     NNADQTFQNGYNIAKVELALGDPAICALIAGCTPLDLFGGQGRQMTPEMINYIRATQI
                     DSSKQTLELVTANITGDLFDIGDRTAGFAVGAEHRKYVGEFSPDPLRQTGESQDSFAS
                     PVSESYDVNEVYAEFSVPLLETLDVSAAVRWSDYSTFGSETTGKAGLRWQPIEALAFR
                     GTYSKGFRAPNLGELFGLTQFAATLVDPCGPTGTVVVNDADGLNTTALETACRAQGVP
                     SGFEQANTQITTFTGGNPELQPEKSDSYTAGIVHDATWAESWSSRLTFEITYFSHEID
                     SGIQARDIQALINACLAAGGADPTLCSPFTRQAGGNLNPPNNFLDNLGTIETDGFDFK
                     IDWRGTERSWGTMSVGLQATFVNDYTATDTDGITSQRTVGIEVSDSAIPELQANMQIG
                     WERGAWEASFLTRFIDSVDEYCGNSLTTNVPGCSTGQQFHTLGSAIYNDAQVGWSKAF
                     GLENFKLALSVNNIFGEDPPICYSCSLNGYDAGTYDLPGTFWAVTAKFGF"
     gene            complement(4959..5303)
                     /locus_tag="VIV63_05765"
     CDS             complement(4959..5303)
                     /locus_tag="VIV63_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEY6124136.1"
                     /translation="MRMTIYGIRNCDTMKKARAWLESHGVAHEFHDYKIAGIDRAKLE
                     AWAKIVGWEVLLNRAGTTFRKLPEDAKTGLTEARAIKLMIEQPSMIKRPVLERGKSLI
                     VGFSPEKYSALK"
     gene            complement(5318..6595)
                     /locus_tag="VIV63_05770"
     CDS             complement(5318..6595)
                     /locus_tag="VIV63_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6124137.1"
                     /translation="MTELERSTMARVTWRLLPFLLLLYIISWLDRVNVGFAKLQMNSD
                     LGMSDTAYGFGAGIFFLAYAACEIPSNLLLVRFGARRWIARIMITWGLISAGMMFVQG
                     EKSFYVMRLLLGAAEAGFLPGIVYYLSQWFPRPERARAVSWFMIGIPLSIVFGGPLSG
                     WLLGFEGHLGLHGWQWMFLLEGLPAVVLGFVVLGYLTDKPADAKWLTAEQREWLSRTI
                     EAEHLVAQARHRVSVREVLEHPTVWLLAAIMFCCQTGSYGLTLWVPTIVKGLSGFNNF
                     EVGLISALPYIAAAAGMVLIGRSSDRTGERFMHVAIPTAIGAIGFVFTAILKSPIPAM
                     IALTVAAVGDYGTRGPFWAMPGKFLTGSAAAAGIALINAMGAVGGFVGPYAVGYLKDT
                     SGNFESGLFLLAGILFAGSVLTLFLRKSATLAD"
     gene            6651..7742
                     /locus_tag="VIV63_05775"
     CDS             6651..7742
                     /locus_tag="VIV63_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6124138.1"
                     /translation="MSEPAGEWIDVGAAEDFKRTPLREAKLGERLVAVSYQDGKFGVI
                     SNVCNHVGGPLGKGRLDGEFVVCPWHQYKFNRCTGEGEPGYEADKVPGYGVREENGRL
                     LVTAKPTSKRGHLPHEPHPLTRPTERAPGGLRIGGISTTAMDAKNPRFSTSEHLLGVA
                     LDHARESLGAETKLIRLNDLNFRNCEGYYSKAAKACTWPCSVTSMDKTDQLDQVYEMA
                     VHWADILLIATPIRWGAASSLYYKMVERMNCIQNQITINNKVLIRNKVAAFIITGGQD
                     GIQAVAGQMMGFFSEIGYLLPQFPFIAHSLGWEFEEMERNMDFVQRSEGLRAGARELV
                     TRASEMSKCLLEREIAHATIERGGRKASA"
     gene            complement(7777..9033)
                     /locus_tag="VIV63_05780"
     CDS             complement(7777..9033)
                     /locus_tag="VIV63_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HEY6124139.1"
                     /translation="MSSLDTWTMPPEATAILVALGAGLLIGVERERRKADDAERAAAG
                     VRTFAMTSLLGVLVALANSPVLLGAAALGVVLLTAFSYFKSGSADPGLTTEVAMLATF
                     VTGVLAATQPGLAAAAGVIIVVLLVARGALHGFARRELSEQELQDGVLLAAAALLVLP
                     LLPDHAIDPWQVVNPRLVWKLTVLIMLVDAAGYVAQRLVGPRAGLPISGLLGGFVSST
                     VVVATMGTRSRSEPEVLPAAVAGAALSQVATVIQLALVLAVSDAVLLYELRWALLATG
                     AAMVVYGFVMYRASKGHAEGPPVAGRAFRLRTALLFAGAFSGIALLVAWLQSSFGPAW
                     ALAGVVVGGFADAHSTSASVGTLASQGQMNRDLAAIAVGLIVTTNTLSKLAFARAGGS
                     AYFWRLAPGLVLLIATFWAAGWLIPR"
     gene            complement(9076..10101)
                     /locus_tag="VIV63_05785"
     CDS             complement(9076..10101)
                     /locus_tag="VIV63_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY6124140.1"
                     /translation="MLARVVENVYWLSRYLERAENTARIIGVNTNLLLDLPGGIAPGW
                     LPLVDISGNRAEFDVKGTKGEERDVVNFLIADKENPGSICSSLVAARENARTLREILP
                     TEAWELLNEFFAEFNKDLAAGINKRTRFDFLKRVVVTLQTLSGMLDGTMNRNDAHTFS
                     TLGRNLERADMTSRIVDVRSAQLLPAETPELRPFETVQWMSVLKSLSGYQMYRLKMRT
                     RVKRTDVLQFLLRDDQFPRSCLYCLTQLESSLSPLPRSEGALEVLDGAAKFIQRAPLA
                     TLDQPGLHELIDDIQLRISNVHSRLAEIYFPSGEISGMQRMSGQSQSQSQSQRQTSLS
                     FGESAKA"
     gene            complement(10101..>11427)
                     /locus_tag="VIV63_05790"
     CDS             complement(10101..>11427)
                     /locus_tag="VIV63_05790"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY6124141.1"
                     /translation="IAVRQQAAELAIKAMGITFTVYHEQDGSIDRAWPLDIIPRTITR
                     KEWQKIEAGLIQRAAALNLFINDVYHDQKFIKDGLMPASIIATSKNFRASAKGIKPPL
                     GTWAHICGSDLIRDKDGTVYVLEDNLRVPSGVSYMLENRQVVKRVFPELFESAAILPV
                     DDYPSQLYDMLAALSPRKETRPEVVVLTPGIYNSAYFEHSYLARQMGCELVEGRDLAV
                     DSDDCVYMQTVRGWTRVDVIYRRIDDDFLDQEVFRKDSSLGVPGLMRAWAAGKVALAN
                     APGAGVADDKLVYTYVPDMIKYYLGEDAILPNVPSYRCGDAKQLEHVLANLDHLVVKP
                     VDGSGGYGMLMGPMSTKAERAKFADSLKADPRSFIAQPIVTLSTVPTLVNDRLEPRHV
                     DLRPFILSGPQTAVTTGGLTRVALRKGSLVVNSSQGGGSKDTWIVDTDN"
BASE COUNT         2117 a         3633 c         3704 g         1973 t
ORIGIN      
        1 tcaagactcc ggcgtttccg ccgcctcgct cgaactcgag atcacggaaa gcgcgatgat
       61 gctcgacccg gcgcgcgcgc ttcgcgtcat ccaggaaatc gacgagctgg gcgtgttcat
      121 ctccatcgat gacttcggta ccggctattc gtcgctcggt tacctgcgcg acctgcccgt
      181 gcactcgctc aaactcgaca agtcgttcgt catgggaatg cgcaacaact ccgacgatcg
      241 cgtgatcgtg gaatccaccg cgcagatggc gcacgcgctc aagctggagc tggttgccga
      301 aggcgtggaa accgagtggg atgcacagtt cctggcggcc gccggctacg actacgcgca
      361 gggtttccgt tacacccgcg cgttgcccga cgaccagtgc ctggcgtgga tcgtggagtt
      421 caacgccacc gcgatgctga cgctgggcga tgccgacgtg accgccacgc tgcgatcacg
      481 cgccgcgcgc gacgacgaag acgatttcga atcgcgaacc gcctaggtct gcggtttgag
      541 ccgtggcacc cacgcgaatc ccatctggaa gccgatgtcc ggcgtgttgt tgaaattctg
      601 caggttctcg gtgatgccga acgacaccag gcagcaatcg aagcggtggc gcacgcccag
      661 gctcagctgg aacttgtcgc tgagcaattc gtcgagatcg gtctgcgcgg gcttgtagac
      721 gctggtgctc gcatacgcct gtaggttcag gttggtacgc gcggacatct gccgttccca
      781 gccgaccacg atcgtcggaa tgatctgcga ttcgttcggc gccggcaggt cctcgccggc
      841 gtaatagacc gcggcgaaat cgatgtgcaa cgcattgcgt tcgccgaggc ggcgaaccga
      901 ggcttgcatc ccgtagtcgg tgttgccggt cgacagcagt gggcgcgcgc cgctgatcgg
      961 tatcttcacc gcggtttcca ccgacatctg ccatcggccc ggcagcgaga agccgctgta
     1021 gcgcagtccg aaagtcggat cgaggaagcc tgaactgctc ggcggttcgt tcatcaccag
     1081 ctgcgagctc ttcaggtcga agatgacatg cgttccgttg cgcgcgatgg cggggcggcc
     1141 gaatgagctc aagcccacca ggtcgtggaa tccttcgatg gtcgagtcga ggaagccgcc
     1201 ttgataggac acggcggtgg cgaacgcgta cgcggtccac tggctggaga tcttgtagtg
     1261 cacggccagg tcgagcgtcg cggactcgag atcgagcagg tagttttcgc ccggcagatc
     1321 gcggatggcg gcgatttccg ccggaccgat ctcgcggcgg ccagaactct cgagcgacgt
     1381 gaggtacttc tccacgttct cgctgagcgc ccaggtgttc tggtagccga gctcggcttc
     1441 gaacgcgaaa gtgtgtttct cgatgttgac ggcgtgcgcg ggacgcatgt ccaggcgcag
     1501 gaatccgaaa ggcgtcagat cacgcgagcg caacatgccg tagaagcgac cgctttcatc
     1561 ggcttgtgcg gaatttacac atcccaccgc caaggccaga ccacacgcgg ccaccctggc
     1621 cagcttgttc ttcatcgaga ctccctttcc ctcgcgacga cagatttacc agcgtgcgcg
     1681 cggcgtggca atcggacagc ggagagacaa tccgcgggga catgagcgtt gcatgcaatc
     1741 cacaaccggt cgttcatgct ggcgcaaagc ccagtttccg gtcgcacatt agccacagca
     1801 gggctttcct tttgtcccgg tgaagcgtat ttatgccacc acttcgccgg caggagggga
     1861 cggcgatcca acacaccagg aaggtctgta tgaaaaagca aagcaagaat cggcccgcat
     1921 tgacggctct gcttccctcg ctgctggtcg ccgccaccgc gtcggcccag gacgcgcccg
     1981 cggaccccac gatgcaggaa gtagtcgtca ccggctcacg catcatccag tcgagcgcca
     2041 actcccaaca acccatttcg atcatcgacc gcgccaccat cgacaagacc ggcctcgcca
     2101 gcatcgggga gctgctgcag caggtcacgg cgggcggcaa ggcgctcaac gccaaattca
     2161 actcttccgg caacttcgga tatcccgctg acggcggcgg catcggcgcg ggttccgcgc
     2221 aggtcgacct tcgcaacctc ggcagcaaac gcgtgctcgt gctcgtcgac ggcatccgtt
     2281 gggtgaatga atcgtcggcg tccggcgtgt ccggcgcggc cgacctcaac accattccca
     2341 tgagcatcat cgagcgcgtc gaagtgctcg aagacggcgc gtcggccatc tacggttccg
     2401 atgccatcgc cggcgtggtg aacatcatca cgcggaagga attcgaaggc gtcgagctca
     2461 atggttattt cggcgaatac gaattgggcg ggtcgaccac cgacgccagc ttcacgctcg
     2521 gcggcggagg cgagcgcttc cgcggtttgt tcgtcgcgag ttactacgac caggaaggca
     2581 tcgattcggg cgagtgggac cagtcctcgt tcccgcagcc gaacgcgggc ctctcggccg
     2641 gcagctcggc gaccccgcag ggccgttacg gcttctgcga tccggcgcgc cctgccgcca
     2701 gctatggctc gtgcagcgcc agccaggaat tcttctacga cgtcacgttg aacaacggca
     2761 ccacgacgcc ggtctggaac cccgccgatc cgtccgcggg ctcgtatcac gacttcagtg
     2821 gcgcggaccg tttcaactac gcgccttaca acctgctgct gacaccctcc gagcgcaagt
     2881 cgatgtttgc gcgcatgtcg ttcgatgtga gcgacatggt caccctcaac gtcaaaggcc
     2941 tgttcaacaa ccggcggtcg accaatcgcg cggcgcccga gcccatcttc gtgggtccct
     3001 atgccggcac cggcggcctc gccgacacca tctcgatttc ggcgctgaat ccgttcaacc
     3061 cgttcggcat cgacctcgac gcgagcagca acttcggctg ggtcacgcgc cgtcccgtcg
     3121 aagtcggccc acgcctcttc gagcaggacg tcgacacctg gtatttcaat ctcggactcg
     3181 aaggcaagtt cggcagtgac aaccgcttcc actgggatct gaacttcgtc agttccgaga
     3241 acaacgccga ccagacgttc cagaacggct acaacatcgc caaggtcgag ctcgcgctcg
     3301 gcgatccggc aatctgcgcg ctgatcgcgg gctgcacgcc gctcgacctg ttcggtggcc
     3361 agggccggca gatgacgccg gagatgatca actacatccg cgcgacacag atcgactcgt
     3421 cgaagcagac gctggaactg gtgaccgcca acatcaccgg tgacctgttc gacatcggcg
     3481 atcgcaccgc gggtttcgcg gtgggtgccg aacatcgcaa gtacgtgggc gagttctcgc
     3541 ccgatccgct acgccagacg ggtgagtcac aggattcgtt cgcctcgccg gtgtcggaga
     3601 gttacgacgt gaacgaggtg tacgccgagt tcagcgtgcc gttgctcgag accctggatg
     3661 tcagtgccgc ggtgcgctgg tccgactact cgacgttcgg cagcgaaacc accggcaagg
     3721 cgggtctgcg ctggcagccc atcgaagcgc tcgcgtttcg cggcacgtac tcgaagggct
     3781 tccgagcgcc gaacctgggt gagctgttcg gcctgacgca attcgccgcg acgctggtcg
     3841 atccttgcgg accgacgggc acggtggtgg tgaacgacgc cgatggtctc aacaccacgg
     3901 cgctcgaaac cgcctgccgc gcgcagggcg tgccctcggg cttcgaacag gcgaacacgc
     3961 agatcaccac tttcaccggt ggcaacccgg agctgcagcc cgagaaatcg gacagctaca
     4021 ctgcaggcat cgtgcacgac gccacgtggg ctgagagctg gagctcgcgc ctgacgttcg
     4081 agatcaccta cttcagccac gagatcgaca gcggcatcca ggcgcgcgac atccaggcgc
     4141 tgatcaacgc ctgcctcgcg gccggcggcg ccgatccgac gttgtgttcg ccgttcacgc
     4201 gccaggcggg cggcaatctc aatccgccta acaacttcct ggacaacctc ggcaccatcg
     4261 aaaccgacgg cttcgatttc aagatcgact ggcgcggcac cgaaagatcc tggggcacga
     4321 tgagcgtggg cttgcaggcg acgttcgtga acgactacac ggccacggac accgacggca
     4381 tcacctcgca gcgcaccgtg ggcatcgagg tgagcgacag cgccatcccg gagctgcagg
     4441 ccaacatgca gatcggctgg gagcggggcg cctgggaagc cagcttcctg acgcgcttca
     4501 tcgattcggt ggatgagtac tgcggtaatt cgctgaccac gaacgtgccg ggttgcagca
     4561 cgggccagca gttccacacg ctcggctccg ccatctacaa cgatgcacag gtgggctgga
     4621 gcaaggcgtt cggtctggaa aacttcaagc tggcgctgtc ggtcaacaac atcttcggcg
     4681 aggatccgcc gatctgctac agctgctcgc tgaacggata cgacgcaggt acctacgacc
     4741 tgcccggcac gttctgggcg gtgaccgcca agttcgggtt ctaagaagtc gaggggggac
     4801 tggagacggg ccgggcggat ttcagcggaa tccagcgcga gtctcctttg aatcgggcgc
     4861 cgtccggagt aaccgggcgg cgcctttttc gttccgcaaa aaaaggggac agatctattt
     4921 ttgtctttcc gttcgcaaaa atagatctgt cccctttttt acttcagcgc cgagtacttc
     4981 tccgggctga agcccacgat cagcgacttg ccgcgttcga gcaccggccg cttgatcatc
     5041 gacggctgct cgatcatgag tttgatggcg cgggcctcgg tcagcccggt cttcgcgtct
     5101 tcgggcaact tgcgaaacgt ggtgccggcg cgattcagca gcacctccca acccacgatc
     5161 ttcgcccaag cttcgagttt cgcgcgatcg atgccggcga tcttgtagtc gtggaattcg
     5221 tgcgccacgc cgtgcgactc gagccaggcg cgcgccttct tcatggtgtc gcagttcctg
     5281 atgccgtaga tagtcattct catgggcgac gatggaccta atcggcgagc gtcgcggact
     5341 tgcgcaggaa cagcgtgagg acggagcctg cgaacaggat tcccgcgagc aggaacagtc
     5401 cgctctcgaa gttgccggat gtgtccttga gataacccac ggcgtacggg ccgacgaagc
     5461 cgccgacggc gcccatggcg ttgatcagcg caatgcccgc ggcggcggca ctgccggtca
     5521 ggaattttcc cggcatcgcc caaaacgggc cgcgcgtgcc gtagtcgccg accgcggcca
     5581 ccgtcagcgc aatcatcgcg ggaatcggtg acttcagtat cgcggtgaag acgaagccga
     5641 tcgcgccgat ggcggtggga atggcgacgt gcatgaatcg ctcaccggtg cgatcggagc
     5701 tgcgcccgat gagcaccatg ccggccgccg ccgcgatgta tggcagcgcg gagatcaggc
     5761 ccacttcgaa attgttgaac cccgaaagtc ccttgacgat cgtgggcacc cagagcgtga
     5821 gtccgtaact gccggtctgg cagcagaaca tgatggcggc gagcagccac accgttggat
     5881 gctcgagcac ctcgcgcacg ctcacgcggt gccgcgcctg cgccacgaga tgttcggctt
     5941 cgatcgtgcg actcagccac tcgcgctgtt ccgcggtcag ccatttcgca tcggcgggtt
     6001 tgtcggtgag gtaacccagc acgacgaacc cgaggaccac cgcgggcagt ccttcgagca
     6061 ggaacatcca ttgccagccg tgcagtccga gatggccctc gaagcctaac aaccatcccg
     6121 acagcggccc gccgaacacg atggacagcg ggatgccgat catgaaccac gacaccgcgc
     6181 gtgcgcgctc cggccgcggg aaccactggc tcaggtagta gacgatgccg gggagaaacc
     6241 ccgcttcggc ggcgccgagc agcagccgca tgacgtagaa actcttctcg ccctggacga
     6301 acatcatgcc ggcggagatg agtccccacg tgatcatgat gcgcgcgatc cagcgccgtg
     6361 cgccgaaacg cacgagcagc aggttgctgg ggatttcgca ggccgcatag gcgaggaaga
     6421 agatgcccgc gccgaaacca taggccgtgt cgctcatgcc gaggtcgctg ttcatctgca
     6481 gcttggcgaa gccgacgttg acgcgatcca gccaggaaat gatgtacagc agcagcagga
     6541 agggcagcag gcgccaggtc acgcgcgcca tggtcgagcg ttcgagttcg gtcatcgccc
     6601 gagtgtaggg gatatgattg cggccagctt caggattgcg cggagtggat atgagcgagc
     6661 cagcggggga gtggatcgac gtcggcgcgg ccgaagattt caagcgcacg ccgttgcgtg
     6721 aggcgaagct cggcgagcgg ctcgtggccg tctcgtacca ggacggcaag ttcggcgtga
     6781 tctccaacgt gtgcaatcac gtcggcggac ccttgggcaa aggccggctc gatggtgagt
     6841 tcgtggtctg tccgtggcac cagtacaaat tcaatcgctg caccggcgag ggtgagcccg
     6901 gctacgaggc cgacaaggtt cccggctatg gcgtgcgcga agagaacggc cgcttgttag
     6961 tcacggccaa accgaccagc aagcgcgggc acctgccgca cgagccgcat ccgctcacgc
     7021 ggcccaccga acgcgcgccc ggcggcctgc gcatcggcgg catttccacc accgccatgg
     7081 acgcgaagaa cccgcgattc tcgacgtccg aacacctgct gggcgtggcg ctcgaccatg
     7141 cgcgcgagtc gctcggcgcg gaaacgaagt tgatccgtct caacgatctc aacttccgca
     7201 attgcgaggg ctactactcc aaagcggcga aggcctgcac ctggccgtgt tcggtcacgt
     7261 cgatggacaa gaccgaccag ctcgaccagg tctacgagat ggccgtgcac tgggccgaca
     7321 tcctgctcat cgcgacgccc atccgctggg gcgcggccag ttcgctctac tacaagatgg
     7381 tcgagcgcat gaattgcatc cagaaccaga tcaccatcaa caacaaggtg ctgatccgca
     7441 acaaggtggc ggcgttcatc atcacgggcg gacaggacgg catccaggcg gtggcggggc
     7501 agatgatggg attcttcagc gagatcggct atctactgcc gcagttcccg ttcatcgccc
     7561 actcgctcgg ctgggagttc gaggagatgg agcggaacat ggatttcgtg cagcgtagtg
     7621 agggcctgcg cgccggtgcg cgcgaactcg tgacgcgcgc gtcggagatg tcgaagtgcc
     7681 tgctcgaacg cgagatcgcc catgccacca tcgagcgtgg cggtagaaaa gcctccgcct
     7741 gacggcgccc aggagctgcc ctggttcgcc attgcatcaa cggggaatga gccaccctgc
     7801 cgcccagaac gtcgcgatga gcaacacgag tcccggcgcg aggcgccaga aataggcgct
     7861 gccccccgca cgcgcgaacg ccagtttcga cagggtgttg gtggtcacga tgagtccgac
     7921 ggcaatcgcc gcgaggtcgc ggttcatctg gccttgcgag gcgagcgtgc ccacggacgc
     7981 ggacgtggaa tgcgcgtcgg cgaagccgcc gaccacgaca ccggcgagcg cccatgccgg
     8041 cccgaagctc gattgcagcc aggcgaccag cagcgcaatg ccgctgaaag cgccggcgaa
     8101 caacagtgcg gtgcgcaggc gaaacgcacg gccggccacc ggcggcccct ccgcatgacc
     8161 cttggaggcg cgatacatca cgaatccgta gaccaccatc gctgcgccgg tcgcgagcag
     8221 cgcccagcgc agttcataga gcagcaccgc gtcgctcacg gccagcacca gcgcgagctg
     8281 gatcacggta gccacctgcg acagcgccgc gccggccacc gcggccggca gcacctcggg
     8341 ctcgctcctg gaccgcgtgc ccatggtggc gaccacgacc gtgctcgaca cgaacccgcc
     8401 taacaacccg gagatcggaa gcccggcgcg cgggcccacg aggcgctgcg ccacgtatcc
     8461 cgcggcgtcc acgagcatga tgagtacggt cagcttccag acgaggcgcg ggttcacgac
     8521 ttgccaggga tcgatggcgt gatcaggcag gagtggcagc acgagcagcg cggccgccgc
     8581 cagcaatacg ccgtcctgga gctcctgttc cgagagctcg cgccgcgcga acccgtgcag
     8641 ggcgccgcgc gccaccagca gcacgacgat gatcacgccg gcggctgccg ccagtcccgg
     8701 ctgcgtcgcg gccagtacgc ccgtgacgaa agtcgcgagc atcgcgacct cggtagtgag
     8761 accgggatcc gcgctgcctg atttgaaata ggaaaaggcg gtgagcagca ccaccccgag
     8821 cgccgcggcg ccgagcaaca ccggtgagtt ggcgagagcc accagcacgc ccagcaatga
     8881 agtcattgcg aaggtgcgta ccccggcggc cgcgcgttcg gcatcgtcgg ccttgcggcg
     8941 ctcacgctcg acgccgatga gcaggccggc cccgagcgcg accaggatgg cggtggcctc
     9001 gggaggcatc gtccaggtgt ctagtgatga catggacgcg ctgcatatca cgcagcgccg
     9061 cgagccgcaa cgcgctcagg ctttcgcgct ttctccgaac gacagcgatg tctgccgctg
     9121 cgactggctt tgcgattgac tctggccgct catgcgctgc atgccgctga tctctcccga
     9181 agggaagtag atctcggcca gccgcgagtg aacgttggag atgcgcagct ggatgtcgtc
     9241 gatgagttcg tgcaggccgg gctgatcgag agtagcgaga ggtgcgcgct ggatgaactt
     9301 ggcggcgccg tccaggactt cgagtgcgcc ttcgctgcgc ggcagcggcg agagcgagct
     9361 ttcgagttgc gtgaggcaat acaggcagga acgcgggaac tggtcgtcgc gcagcaggaa
     9421 ctggagcacg tcagtgcgct tgacgcgtgt tcgcatcttc agccggtaca tctggtagcc
     9481 cgacagcgac ttgagcacgc tcatccattg cacggtttcg aacggccgca gctcgggagt
     9541 ctccgccgga agtagttggg ctgagcgcac gtcgacgatg cggctggtca tgtcggcccg
     9601 ctcgagattg cgccccagcg tgctgaaggt gtgcgcgtca ttgcggttca tcgtgccgtc
     9661 gagcatgccg gacagggtct gcagcgtgac gaccacgcgc ttcaggaagt cgaagcgggt
     9721 gcgcttgttg atgcctgccg caagatcctt gttgaactcg gcgaagaatt cgttgagcag
     9781 ttcccacgct tcggtgggca ggatctcgcg cagcgtgcgc gcattttcgc gcgccgcaac
     9841 caacgagctg cagatcgacc cgggattttc cttgtcggcg atgaggaagt tgacgacatc
     9901 gcgctcttcg cccttcgttc ccttgacgtc gaactcggcg cggttgccgg agatgtcgac
     9961 cagcggcagc cagcccgggg caatgccacc ggggagatcg agcagcaggt tggtgttcac
    10021 gccgatgatg cgcgcggtgt tctccgcgcg ttcgaggtag cgggacaacc agtaaacgtt
    10081 ttcgacgacg cgggctaaca tcagttgtcc gtatccacga tccaggtgtc cttgctgccg
    10141 ccgccctgcg aggaattcac caccagcgag cccttgcgca gcgcgacgcg cgtgaggccg
    10201 cccgtggtga cggcggtctg cggaccggag aggatgaacg gacgcaggtc cacgtgccgc
    10261 ggttcgagcc tgtcgttcac cagcgtcggc acggtagata gtgtgacgat gggctgcgcg
    10321 atgaagctgc gcggatccgc cttgagcgaa tcggcgaact tggcgcgttc ggccttggtc
    10381 gacatggggc ccatgagcat gccgtagccg cccgagccgt cgaccggctt cacgacgagg
    10441 tggtcgaggt tggcgagcac gtgttcgagc tgtttggcgt cgccacatcg gtacgacggc
    10501 acgttgggca ggatggcgtc ctcgcccagg tagtacttga tcatgtcggg cacgtacgta
    10561 tagacgagct tgtcgtcggc gacgcccgct ccgggtgcat tcgccagcgc cactttgccc
    10621 gcggcccagg cgcgcatgag tcccggcacg cccagcgagg aatctttgcg gaagacttcc
    10681 tggtcgagga agtcgtcgtc gatgcgccgg tagatgacgt ccacgcgagt ccagccgcgc
    10741 acggtctgca tgtacacgca gtcgtcgctg tcgaccgcga gatcgcggcc ttcgacgagt
    10801 tcgcaaccca tttgtcgcgc gaggtaggaa tgttcgaaat acgccgagtt gtagatgccc
    10861 ggcgtgagca cgaccacttc agggcgagtt tccttgcgcg gagacagtgc agccagcatg
    10921 tcgtagagct gcgaggggta atcgtccacc ggcaggatgg cggcgctctc gaacagctcc
    10981 gggaacacgc gtttgaccac ctggcggttc tcgagcatgt aggaaacgcc ggaagggacg
    11041 cgcaggttgt cttcgagtac gtagaccgtg ccgtccttgt cgcgtatcag gtccgagccg
    11101 cagatgtgcg cccaggtgcc gagcggcggt ttgatgcctt tggcgctcgc gcggaagttc
    11161 ttggaggtcg cgattatcga ggccggcatg aggccgtcct tgatgaattt ctgatcgtgg
    11221 tagacgtcgt tgatgaacag attgagcgcc gcggcgcgct ggatgagccc cgcctcgatc
    11281 ttttgccatt ccttgcgggt gatggtccgg ggaatgatgt cgagcggcca ggcgcggtcg
    11341 atcgacccgt cctgctcgtg gtagaccgtg aacgttatgc ccatggcctt gatggccagt
    11401 tcggccgcct gctggcgcac ggcgatc
//
LOCUS       DATDEU010000234         6525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1207214, whole genome shotgun sequence.
ACCESSION   DATDEU010000234 DATDEU010000000
VERSION     DATDEU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207214"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VIV63_05795"
     CDS             <1..293
                     /locus_tag="VIV63_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6124142.1"
                     /translation="DAEIIDVRTMSPLDIDPICSSVKKTGRCVVAHEATRFSGFGAEL
                     SASIQEECFWYLEAPIQRVAGWDTPYPHAFEWEYFPGQARVALALRAAMGQE"
     gene            304..1584
                     /locus_tag="VIV63_05800"
     CDS             304..1584
                     /locus_tag="VIV63_05800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY6124143.1"
                     /translation="MSRYVFKMPDLGEGTVSAEVVAWHVKPGDLVQEDQVMCEVMTEK
                     AAVEMPAPVTGRILSIQGAPGDMVAVGSELVVFDTDATSAAAGAEPVAKTPLPPATEK
                     QPGEKQPGEKQQVAAAAAVNGGNGNDKASRVMVSPASRRRAAEAGLDLSTVAGTGPAG
                     RIEPGDIDSALAAGAARGTPPSINARANAPHEPRNGTEEVKIIGLRRVIAERLTESAR
                     SIPQYSYVEECDLTRLEGLRKHLNDRRPPSAPPLTFLPFIVAALARVLDKFPQCNALY
                     DSTRGVLVKHKAVHVGVATQTPQGLKVPVVRNAESRALHDLAAEIRRVAEAARTNKSP
                     REELTGSTITITSLGKLGGIASTPMINMPEVSIIGINKAIERAVVVDGQICVRLMMNL
                     SSSFDHRFVDGFDAASMIQALKDSLEQPATIFID"
     gene            1590..2987
                     /locus_tag="VIV63_05805"
     CDS             1590..2987
                     /locus_tag="VIV63_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124144.1"
                     /translation="MRSLCLALLLLVVNRADAQYKGLSGTAQVRVEITRSGSEWVAEY
                     QFDREAPVWVFPRTDVTREDRQEWRPLSWTVETRGVRLERRGWYDVLVARDKVPRNVR
                     IRFTPFPQGLQADYTPALEFTDDSVALYIAHFETFPMESIARVKALPSDLNNQLVPDA
                     NVTYVFRDRAGPVWLRGKRLAVAETTDKNTYVFFGSMKPIETRDMVGFIDPELPEWIR
                     GSLVETVPDMLARYSRELGAPPGMKPTIMLSWAGPTPGLTSRAGSVLPGLIVLTYEGS
                     GVLSEDDAARIEGLQFIAHEAAHFWLGQTVAYEYAKDAWITEGGADLLAMRLMESAHV
                     GYDWRTDFNRAIEECVGFTRRRGIESARERNETRAYYACGAVFGLIAESASGRSFFTF
                     VKRIVDSNRADGIVTRAEWLAALDSASRKPDLSRDIARLLDRGAPDPKVFIAALFTRA
                     GVQHSIDAEGMPHLP"
     gene            3061..3345
                     /locus_tag="VIV63_05810"
     CDS             3061..3345
                     /locus_tag="VIV63_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124145.1"
                     /translation="MHSRCKSCLYCGFVNYVSLGDPPPITKWKRPVLFVAGIALAVCT
                     VLALRFAWERTDGTFFAAVSALLNIIALIGVAIAIRGCDVCVARYLGKTL"
     gene            3595..5541
                     /locus_tag="VIV63_05815"
     CDS             3595..5541
                     /locus_tag="VIV63_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEY6124146.1"
                     /translation="MKTGLLLGLLSVLTAASQAAEPAPGDWPNYGRTPGGDRHSPLEQ
                     IDRANVGKLTLAWEFKTGEADIKTGNPTALEATPLVVDGVMYLSTPLGKVFALDPVTG
                     KQRWVRDVGVKRERHFGDWVSRGVSYWRDSRAKPTAKCAQRIFFGVVDARMVALDAAT
                     GEFCRDFGNAGEINLVPLLRNPQSYGDEYEQTSPPAVIGDLIVAGSAIADNNSNVGST
                     GEVRAFDARTGALRWTWNPVPQDPTDPAYKTWAGSAAHRTGGANTWSIIAADPQRDLV
                     FLPTTSPAVDYYGVTRLGDNRYANSVVALRASTGKLVWHFQTVHHDLWDYDNAAPPAL
                     ITLPDGRAAVLQGTKTAQLFVLDRETGAPLFPVTEIPVPKSDIPGEVTSPTQPLSSLS
                     TGFRTLTAADIWGATPEDLAECRARFATLRYDGPFTPPSERGTVQVPANIGGSHWGGV
                     AYDSERGIAIVPNNRLAAVVKLIPRETWKVLREKLTSGERVGKEFTDMQGTPYIMQRE
                     TWLSSRRSPCTAPPFGTLTAISLKTGKTLWDVPLGNSDGLDKIGIPPLPADSGMANLG
                     GAITTSGGIVFIGATLDAWFRAFDIESGRELWKFKLPAGGKATPSTYLGADGRQYVVI
                     SAGGDGKSWGKADSVMAFALPKTP"
     gene            5588..>6525
                     /locus_tag="VIV63_05820"
     CDS             5588..>6525
                     /locus_tag="VIV63_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124147.1"
                     /translation="MAQTFDIRFARSAGLAALLEAPENRFRWKGGGLLRIDAQSISFT
                     VKRGLLALFAGKQTRRIPAEDLKAVYREGDALRVEFQTDETARMVLPFWADDREAAAQ
                     IVRLLPTSQTVEIEHTTGSSTPRADGRVMLLIGAALAALVAVAWVLYPRTELPVAAVT
                     VPEIADAAIADATSPGAADVVPSVPLPTSVDDFLIEKPAASSQSYIPELAIPDSVKQP
                     PVLVEPPARLPLSPDRARSEDFVIRIERDTAAFHVAKTQLAAFEQDANQLEARWEALR
                     DSFKASALTAEEFAAQLDDLEMRWWDVSFRLFDNEAL"
BASE COUNT         1158 a         2176 c         2107 g         1084 t
ORIGIN      
        1 tcgacgccga gatcatcgac gtgcggacga tgtcgccgct cgacatcgac ccaatctgtt
       61 cttcggtcaa aaagaccggc cgctgcgtgg tcgcgcatga agccacgcgg ttcagtggtt
      121 tcggcgcgga actctcggcc agcatccagg aagaatgctt ctggtatctc gaagcgccga
      181 tccagcgcgt ggccggctgg gatacgccat acccgcacgc gttcgaatgg gagtattttc
      241 ccggacaggc acgagtggcg ctcgcgctgc gcgcagcgat gggccaggaa tgagaggcag
      301 gaaatgagcc gctacgtatt caaaatgccg gacctgggtg aaggcacggt ctcggccgaa
      361 gtcgtcgcct ggcacgtgaa gcccggcgat ctggtgcagg aagaccaggt catgtgcgaa
      421 gtcatgaccg aaaaggccgc ggtggaaatg ccggcgccgg tcacgggccg catcctctcc
      481 atccagggcg cgcccggcga catggtcgcc gtgggttcgg agctcgtggt gttcgacacc
      541 gacgccacat ccgccgccgc gggcgcggag ccggtcgcaa aaacgccgct gccgcctgcc
      601 accgaaaagc agcccggaga aaagcaaccc ggagaaaagc aacaagtcgc cgcggctgcc
      661 gccgtgaacg gcgggaatgg caacgacaag gcctcgcgcg tcatggtctc gccggcgtca
      721 cgccgtcgcg ccgccgaagc cggcctcgat ctatctaccg tcgcgggcac gggtcctgcg
      781 ggccgcatcg aaccaggcga catcgactcc gcgctcgccg ccggtgcggc gcgtggtacg
      841 ccgccgtcca tcaatgcgcg cgccaacgcg ccgcacgagc cgcgcaacgg cactgaagaa
      901 gtgaagatca tcggcttgcg gcgcgtcatc gccgagcgcc tcaccgaatc cgcgcgcagc
      961 atcccgcagt actcctatgt agaagagtgc gacctcacgc gcctcgaagg actgcgcaaa
     1021 catctcaacg accgccggcc gccttccgcg ccgccgctca cgttcctgcc gttcatcgtt
     1081 gccgctctcg cacgtgtcct cgacaaattc ccgcaatgca atgcgctgta cgactcgaca
     1141 cgcggcgtgc tggtcaaaca caaggccgtg cacgtgggcg tcgccacgca gactccgcaa
     1201 ggcttgaagg tgcccgtggt gcgcaacgcc gagtcgcgcg cgctgcacga tctcgccgcc
     1261 gagatccgcc gcgtcgccga agccgcgcgc accaacaaat cgccgcgcga agagctcacg
     1321 ggatccacga tcacgatcac cagcctcggc aagctgggcg gcatcgcctc gacgcccatg
     1381 atcaacatgc ccgaggtgtc catcatcggc atcaacaagg ccatcgaacg cgcggtggtg
     1441 gtcgacggcc agatctgcgt gcgcctgatg atgaatctgt cctcgtcgtt cgatcatcgt
     1501 ttcgtcgacg gcttcgacgc cgcctcgatg atccaggcgc tcaaggattc gctcgaacaa
     1561 cccgcgacga tcttcatcga ctgaacgcga tgcgcagcct ctgtctcgcg ctgctgctgc
     1621 tcgtcgtcaa ccgcgccgac gcccaataca aagggctgtc cggcactgcg caggtccgcg
     1681 tcgagatcac gcgcagcggc agcgagtggg tcgctgaata ccagttcgat cgcgaagcgc
     1741 ccgtgtgggt tttcccgcgc accgacgtca cgcgcgaaga ccggcaggag tggcggccac
     1801 tgagctggac cgtcgagacg cgcggcgtgc gtctcgagcg ccgcggctgg tacgacgtgc
     1861 tcgtcgcgcg cgacaaggtg ccgcgcaacg tgcgcatccg tttcacaccg ttcccgcagg
     1921 gactacaggc cgactacacg cctgcgctcg aattcaccga cgattcggtc gcgctgtaca
     1981 tcgcgcattt cgaaaccttc ccgatggaat cgatcgccag ggtcaaggcg ctgcccagcg
     2041 atctcaacaa tcagctcgtg ccggacgcga atgtcacgta cgtgtttcgc gatcgcgcgg
     2101 ggcccgtgtg gctgcgtggc aagcggctgg ccgtcgccga gaccaccgac aagaacacct
     2161 acgtgttctt cggctcgatg aagcccatcg aaacgcgcga catggtcggt ttcatcgatc
     2221 cggaactgcc ggagtggatt cgcggctcgc tcgtcgaaac cgtgccggac atgctggcgc
     2281 gatactcgcg tgaactcggc gcgccgcccg gcatgaagcc cacgatcatg ctgagctggg
     2341 cggggccgac accgggtctc acgagccgcg ccggcagcgt gttgccggga ctcatcgtgc
     2401 tcacctacga agggtcgggc gtcctgtcgg aagacgatgc ggcgcgcatc gaaggactgc
     2461 agttcatcgc gcatgaggcc gcgcacttct ggctcggcca gacggtggcc tatgaatacg
     2521 cgaaggacgc gtggatcacc gaaggcgggg ccgacctgct cgccatgcgg ctcatggaat
     2581 cggcgcatgt cgggtacgac tggcgaaccg acttcaaccg cgcgatcgaa gagtgcgtgg
     2641 gtttcacgcg gcggcgcggc atcgagtccg cgcgcgaacg aaatgagacg cgcgcgtact
     2701 acgcctgcgg cgcggtgttc gggctcatcg ccgaaagcgc ttcgggccgg tccttcttca
     2761 cgttcgtgaa gcggatcgtc gatagcaacc gggcggacgg catcgtgacg cgggccgagt
     2821 ggctggccgc gctcgattcg gcgtcccgca agcctgattt gagccgcgac atcgctcgcc
     2881 tgctggatcg cggagcgccg gatccgaagg tgttcattgc ggcactgttc acacgggctg
     2941 gcgtgcagca cagcatcgat gcggaaggca tgccgcattt gccctagtgg gctggcgaca
     3001 cctacaaaaa actgcgaacg ggtaccgcct cgtccgcggc cccggtggca catttccatc
     3061 atgcattcgc gctgcaaatc ctgcctgtac tgcggcttcg tcaactacgt gagcctcggc
     3121 gacccgccgc ccatcacgaa gtggaagcgg ccggtgctgt tcgtggcggg aatcgcgctc
     3181 gcggtttgca ccgtactcgc gctgcgcttc gcctgggaac gcaccgacgg cacgttcttc
     3241 gcggcggtct cggcgctgct gaacatcatc gcgttgatcg gcgtggcgat cgcgatacgc
     3301 ggctgcgatg tgtgcgtggc gcggtatctc gggaagacgc tgtgacggtg cgcccggtcg
     3361 tttcctgcaa tccgaagagc cggcgcaagg tgaagaagcg cccgctggga cttcgcgcgc
     3421 cactgcgatc tgcctgccgc tatgcgtcga tctggcagtg ccagtattcc tcgccccgtc
     3481 atcgtcccga tgcggactca aacgccgacg tttgagtgac aatgcgccgg ggattcaaac
     3541 gaatccctcg ccccggcagt ctgatcatga cggccggaaa gatcggggac aagaatgaag
     3601 acgggtctgc tgctcggatt gttgtcggtc cttaccgcgg cctcgcaggc tgccgagccc
     3661 gcgcccggag actggcctaa ctacggacgc acgccgggcg gcgaccggca ttcaccgctc
     3721 gagcagatcg accgcgccaa cgtgggcaag ctcacgctcg cctgggaatt caagactggc
     3781 gaagccgaca tcaagacggg aaatccgacc gcgctcgagg cgacgccgct ggtcgtcgac
     3841 ggcgtcatgt acctgtccac gccgctcggc aaagtgttcg cgctcgatcc ggtcaccggc
     3901 aaacaacgct gggtgcgcga tgtcggcgtg aaacgcgagc gtcacttcgg cgactgggtg
     3961 agccgcggcg tgtcgtactg gcgcgattcg cgcgcgaagc ccacggcgaa gtgtgcgcag
     4021 cgcatcttct tcggagtcgt cgacgcgcgc atggtcgcgc tcgatgcagc cacgggcgag
     4081 ttctgccgcg acttcggcaa cgcgggcgaa atcaatctcg tgccgctgct gcgcaacccg
     4141 caatcctatg gcgatgaata cgagcagacc tcaccgcccg cggtgatcgg cgatctcatc
     4201 gtcgccggct ccgccatcgc cgacaataat tccaacgtgg gctcgactgg tgaagtgcgc
     4261 gccttcgacg cgcgcaccgg cgcgctgcgc tggacctgga atccggtgcc gcaggacccg
     4321 actgacccgg cttacaagac ctgggccgga tccgcagcgc atcgcaccgg cggcgccaac
     4381 acctggtcga tcatcgccgc cgatccgcag cgtgacctcg tattcctgcc gaccacgagt
     4441 ccggctgtcg actattacgg cgttacgcgt ctcggcgaca accgctacgc gaactcggtg
     4501 gtggcgctgc gcgcctcgac cggaaaactg gtctggcact tccagaccgt gcatcacgac
     4561 ctgtgggact acgacaatgc cgcgccgccc gcgttgatca cgctgcccga cggacgcgcc
     4621 gcggttctgc agggtacgaa gaccgcgcag ctcttcgtgc tcgatcgcga gaccggcgcg
     4681 ccgctgtttc cggtcaccga gatcccggtg ccgaagagcg acattccggg cgaggtcacg
     4741 tccccgacgc agccactgtc gtcgctgagc acgggcttcc gcacgctcac cgccgccgac
     4801 atctggggcg cgacgcccga agacctggcc gagtgccgcg cgcgtttcgc cacgctgcgt
     4861 tacgacgggc cgttcacgcc gccgtccgag cgcggaacgg tgcaggtgcc ggccaacatc
     4921 ggcgggtcgc actggggtgg cgtcgcctac gacagcgaac gcggcatcgc catcgtgcct
     4981 aacaaccgcc tggcggcggt ggtcaaactc atcccgcgcg agacctggaa ggtcctgcgt
     5041 gaaaagctca cgagcggtga gcgcgtcggc aaggaattca ccgacatgca gggcacgccg
     5101 tacatcatgc agcgtgagac ctggctgtcg agccggcgtt cgccgtgcac cgcgccgcct
     5161 ttcggcacgc tgaccgccat cagcctcaag accggcaaga cgctgtggga cgtcccgctc
     5221 ggcaacagcg atggactcga caagatcggc attccgccgc tgcccgcgga cagcggcatg
     5281 gccaacctgg gcggcgccat caccacgtcg ggcggaattg ttttcatcgg cgcgaccctg
     5341 gacgcctggt tccgtgcgtt cgatattgaa tcgggccggg aactctggaa attcaaacta
     5401 ccggcgggcg gcaaggccac gccttcgact tatctcggcg ccgatggccg gcaatatgtg
     5461 gtgatctcgg cgggcggcga tggaaagtcc tggggcaagg cggattccgt catggcgttt
     5521 gcactaccga agactccgta gttggaggat agtcggcgtc gatgaggtct cgggggagga
     5581 cgcgagtttg gctcagacgt tcgacatccg gttcgccagg tccgcggggc tcgcagcctt
     5641 gctggaagct cccgagaatc gttttcgctg gaaaggcggc ggcctgttgc gcatcgacgc
     5701 gcagagcatc agcttcactg tcaaacgcgg actgttggcg ctgttcgccg gcaagcagac
     5761 acggcgcatc cccgccgaag acctgaaagc ggtctatcgc gaaggcgatg cgttacgcgt
     5821 ggagtttcag accgatgaaa ccgcgcgcat ggtcctgcca ttctgggcgg atgatcgcga
     5881 ggctgccgcg cagatcgtgc gtctgctgcc gaccagccag acggtggaga tcgaacacac
     5941 cactggcagt tccacgccac gcgccgacgg acgcgtgatg ctgctaatcg gggcggcact
     6001 cgcagcgctg gtggcggtag cctgggttct ctatccccgt accgaattgc cggtggccgc
     6061 ggtcaccgtt ccggagatcg ctgacgcggc catcgccgac gcgacgtcgc cgggcgctgc
     6121 tgacgtcgtg ccatcggtcc cgttgcccac gtccgtggat gattttctga tcgaaaaacc
     6181 cgctgcgtcg tcccagtcgt acatacccga attggcgata ccggattccg tcaagcaacc
     6241 acccgtgctc gtggagccgc ccgcccggct gccgttgtca cccgaccgtg cgcgttccga
     6301 ggacttcgtc atccgcatcg agcgcgacac ggccgcgttc cacgtcgcga aaacgcagct
     6361 cgcggctttc gaacaggatg ccaatcagct cgaagcccgt tgggaagcac tgcgcgattc
     6421 attcaaggcc agcgcgctta ccgccgaaga attcgccgcg caactcgacg atctcgaaat
     6481 gcgctggtgg gacgtgagct tccggctgtt cgacaacgaa gcgct
//
LOCUS       DATDEU010000235         3627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1499896, whole genome shotgun sequence.
ACCESSION   DATDEU010000235 DATDEU010000000
VERSION     DATDEU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499896"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VIV63_05825"
     CDS             <1..534
                     /locus_tag="VIV63_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6124148.1"
                     /translation="LARRTNVGDIAGGDTLIEIADLSEVWVELHAIGNAAARVGVGQK
                     ARISSATSDLHADTTISALLPLATRGQTVIARARVPNTDGRWLPGMTVEADVAVASRE
                     VPLAVRESGLQRFREFTVVFAQSGDTYEVRMLKLGARDGEYAEVLEGLEPGTPYVTQQ
                     SFLIKADIEKSGASHDH"
     gene            545..>3627
                     /locus_tag="VIV63_05830"
     CDS             545..>3627
                     /locus_tag="VIV63_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY6124149.1"
                     /translation="MLERIIRSAVAHRWIVLMLVLGVSALGVWSYSRLPIDAVPDITN
                     VQVQINTEAPGYSPLESEQRITFPVETALAGLPRLQYTRSISRYGLSQVTVVFEDGTD
                     IYFARQQVAERLQQAAMQLPPDVEPTLGPVTTGLGEIFMYTVEGAEPWTQTELRTLQD
                     WVIRPQLRTLKGVTEVNTVGGHVRQIHVMPDPARLIAYELTMADVQRAIERNNSSVGA
                     GYVERSGEQYLIRIPGQIPDIASLGDIVVKSRDGIPLRLADVATIAEGSELRSGAATR
                     DGREIVIGTVFMLIGENSRDVAQRAGAKLREIEATLPPGVTARAIYDRTNLVDRSIAT
                     VRKNLLEGALLVIAVLFVLLGNLRGALITAAVIPVSMLMTISGMVQNRVSANLMSLGA
                     LDFGLIVDGAVIIVENCLRRFGEQQKALGRTLTRAERHELAAQASSEVIRPSLFGLFI
                     IAAVYLPIFALSGVEGKTFHPMAITVVMALTAAMALSLTFVPAAVAQFVSGNVAEQET
                     RVMRAAHGFYAPALQKAIRHRTGLIAAAALLTVFAGWLATRLGTEFIPNLDEGDIAMH
                     ALRIPGTSLTQAVGMQKQLEARLKQFPEVRVVVTKLGTADIATDPMPPSVADTFVLLK
                     DRNEWPDPRKPKSALIAELEEAVHAIPGNNYEFTQPVQMRMNELIAGVRAEVAVKIHG
                     DDLDVLLEAGERIEAVANRIEGAADVKVEQVTGLPLLIIKPDRAALARHGLALADVQD
                     TVAAALAGAHAGQMFEGDRRFDIVVRLPEKMRQDPKTLSTLPIALPAPLAAEDHGTAA
                     PPSFVPLGAVAQIAIEPGPNQISRENGKRRVVVTANVRGRDLGSFVAELRERVSSEVE
                     LPESYWVSYGGTFEQLISASERLSVVVPVVLVLIFGLLFMAFGSARDALVVFSGVPLA
                     LTGGVFTLWLRDIPFSISAGVGFIALSGVAVLNGLVMVSFIRKLLDDGESLDDAIQNG
                     AVSRLRPVLMTALVASLGFVPMALNVGTGAEVQRPLASVVIGGIISSTLLTLFVLP"
BASE COUNT          634 a         1220 c         1175 g          598 t
ORIGIN      
        1 ctcgcgcgac gaacgaacgt cggcgacatc gccggcggcg acacgctcat cgagatcgcg
       61 gacctgtcgg aagtctgggt ggagctgcac gcgatcggca acgccgcggc acgagtcggc
      121 gtcggtcaga aggcacgcat cagttccgcc acgagcgatc ttcacgccga cacgaccatc
      181 agcgccctgt tgccgctggc cacgcgcggc cagaccgtga tcgcccgcgc gcgcgtgcct
      241 aacacggatg gacgctggct gcccggcatg acggtggaag ccgacgtcgc cgtggcgtcg
      301 cgcgaagtgc cgctggccgt gcgcgagtcc ggcctgcagc gattccgcga attcaccgtc
      361 gtgttcgcgc aatccggcga cacctacgaa gtacgcatgc tgaagctggg tgcgcgcgat
      421 ggcgaatacg ccgaagtgct cgagggcctc gagcccggca ccccgtacgt gacccagcag
      481 agtttcctca tcaaggccga catcgagaaa tccggtgcca gccacgatca ctgagaaaac
      541 caccatgtta gaacgcatta tcaggtcggc ggtcgcccac cgctggatcg tcttgatgct
      601 cgtgctcgga gtgtccgcac tcggagtgtg gagctactcg cgcctgccca tcgacgcggt
      661 gccggacatc accaacgtcc aggtgcagat caacaccgag gcgcccgggt attcaccgct
      721 cgaatcggag caacgcatca ccttcccggt ggaaacagcg ctcgcgggcc tgccgcgatt
      781 gcaatacacg cgctccatct cccgatacgg cctgtcgcaa gtcaccgtcg tgttcgagga
      841 cggcaccgac atctacttcg cacgccagca ggtcgccgag cgtctgcaac aggccgcgat
      901 gcaattgccg ccggacgtgg aaccgacgct gggaccggtc accacgggcc tcggcgaaat
      961 cttcatgtac acggtggaag gcgcggagcc gtggacgcag accgaactgc gcacgctcca
     1021 ggactgggtg atccggccgc agctgcgcac gctcaagggt gtcaccgagg tgaacacggt
     1081 cggcggacac gttcgccaga ttcacgtgat gcccgacccc gcgcggctca tcgcctatga
     1141 gctgacgatg gcggacgtgc agcgcgcgat cgaacgcaat aactcgagcg tgggcgcggg
     1201 ctacgtcgaa cgcagcggcg agcaatacct gatccgcatc cccgggcaga tacccgacat
     1261 cgcgagtctc ggcgatatcg tggtgaagtc gcgcgacggc atcccgctgc gcctggccga
     1321 tgtcgccacc atcgccgagg ggtcagaact gcgcagcggc gcggcgacgc gcgacggccg
     1381 cgaaatcgtc atcggcacgg tgttcatgct catcggcgag aacagccgtg acgtggcgca
     1441 acgcgccggc gcgaaactgc gcgaaatcga agccacgctg ccgcccggcg tgaccgcgcg
     1501 cgcgatctac gaccgcacca acctcgtcga ccgcagcatc gccacggttc gcaagaacct
     1561 gctggaaggt gcgctgctgg tcatcgcggt cctcttcgtg ctgctcggca atctgcgcgg
     1621 cgctctcatc accgcggcgg tcatacccgt ctccatgctc atgaccatca gcggcatggt
     1681 gcagaaccgc gtgtcggcca atctcatgag cctgggcgcg ctcgacttcg gactcatcgt
     1741 cgatggcgcg gtcatcatcg tggagaactg cctgcggcgg ttcggcgagc agcagaaggc
     1801 gctggggcgg actctcacgc gcgccgaacg ccatgaactc gccgcgcagg cgagcagcga
     1861 ggtcatacgg cccagcctgt tcgggctctt catcatcgcc gcggtctatc taccgatatt
     1921 cgcgctgagc ggcgtcgagg gaaagacctt ccatcccatg gccatcaccg tggtcatggc
     1981 cctcaccgcc gccatggcgc tgtcgctcac cttcgtgccc gcggccgtgg cgcagttcgt
     2041 cagcggcaac gtcgccgagc aggaaacccg cgtgatgcgc gcggcgcacg gcttttatgc
     2101 accggcgctg cagaaagcca tccgccaccg caccggcctc attgccgccg cggcgttgct
     2161 gaccgtattc gcgggctggc tcgccacgcg tctcggcacg gagttcatcc cgaatctcga
     2221 cgaaggcgac atcgcgatgc acgcgctgcg cattcccgga accagcctca ctcaggccgt
     2281 cgggatgcag aagcagctcg aagcgaggct caagcagttt ccggaagtgc gcgtggtggt
     2341 gacgaaactc ggcacggccg acatcgccac ggacccgatg ccgccgtcgg tggccgacac
     2401 cttcgtgctg ctcaaggacc gcaacgaatg gccggatccg cgcaaaccca agagcgcgct
     2461 gattgcggaa ctcgaagagg ctgttcacgc catcccgggt aacaactacg agttcacgca
     2521 gcccgtgcag atgcgcatga acgaactcat cgccggcgtg cgcgccgaag tcgccgtcaa
     2581 gattcatggc gacgatctcg acgtactgct cgaagccgga gaacgcatcg aggcggtggc
     2641 caatcgcatc gaaggcgctg ccgacgtgaa ggtcgaacag gttacgggct tgccgctgct
     2701 catcatcaag cccgatcgcg ccgccctcgc gcggcacggc ctggcactgg ccgatgtgca
     2761 ggacaccgtg gcggcggcgt tggctggcgc tcacgcgggc cagatgttcg aaggcgatcg
     2821 acgattcgac atcgtcgtgc gcctgccgga aaagatgcgg caggatccga agacgctgag
     2881 cacgttgccg atcgcgttgc ccgcgccgct cgcggccgaa gatcacggca ccgcggcgcc
     2941 acccagtttc gtgccgctcg gcgccgtggc gcagatcgcg atcgaacccg ggcctaacca
     3001 gatcagccgc gagaatggca aacggcgcgt ggtcgtcacc gccaacgtgc gaggccgcga
     3061 cctgggctca ttcgtggcgg aactgcgcga gcgcgtatcc agcgaagtcg aactgccgga
     3121 aagttactgg gtgtcgtacg gcggcacgtt cgaacaactc atctccgcga gtgagcggct
     3181 gagcgtcgtg gtgcccgtgg tgctcgtgct gatcttcggc ctgctgttca tggctttcgg
     3241 ttccgcacgc gatgcgctgg tcgtcttcag tggtgtcccg ctcgcgttga ccggcggcgt
     3301 gttcacgctc tggctgcgcg atattccgtt ctccatttcc gccggcgtcg ggtttatcgc
     3361 gctctcggga gtcgccgtgc tcaatgggct cgtgatggtg agcttcatcc gcaagctgct
     3421 cgatgacggc gagtctctcg acgacgcgat ccagaacggc gcggtctcgc gcttgcggcc
     3481 cgtgctcatg accgcactcg tggccagcct ggggttcgtg ccgatggcgt tgaacgtcgg
     3541 cacgggcgcc gaagtgcaga ggccgctggc ctccgtggtg atcgggggca tcatctcgtc
     3601 caccctgctg acgctgttcg tattgcc
//
LOCUS       DATDEU010000236         8137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_770636, whole genome shotgun sequence.
ACCESSION   DATDEU010000236 DATDEU010000000
VERSION     DATDEU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770636"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VIV63_05835"
     CDS             complement(<1..798)
                     /locus_tag="VIV63_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6124150.1"
                     /translation="MASLASSGNRSSAGARTGLLEGKVAVVYGAAGTMGSAVARAFAR
                     EGAHVELAGRTLEKVQSLATEIAHAGGSVAAAKVDALSPDSIRAHLDAVIVARGRVDI
                     SFNLIGFGGPQGESVLTMPARDVVEPVESGVRTHLLTATAAAHHMSKRHSGVILALTA
                     QVARKPYTGSGGFGVACAAIEGLCRQLAVDLGGMGIRVVALRSSGSPDAPGVSAAIGE
                     HARLAGVTREQFEARIAEKTMLKRMPRMAEVADAAVLMASDRARAITA"
     gene            complement(859..1614)
                     /locus_tag="VIV63_05840"
     CDS             complement(859..1614)
                     /locus_tag="VIV63_05840"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6124151.1"
                     /translation="MNRVVSQQEWIVERKRLMAEEKEFTRRRDALAEKRRAMPWVKIE
                     KDYEFDSPEGRVRFADLFRGRSQLWVQSVMQGPGQPLQCVGCALGIDHIEGLLPHLDN
                     HDVSYVAVARATMPEITALRERMGWRVPFYSSYGSDFNYDFGVSFRPEDLKAGRAVYN
                     YQPCDPGIEDLSGDNVFYRDDAGNIFHTYSCFARGGEDFMTIYRLLEVTPKGRNESPE
                     RGMTDWVRPHDMYGKGGEVAPNGRFTPPVSCCM"
     gene            complement(1625..2257)
                     /locus_tag="VIV63_05845"
     CDS             complement(1625..2257)
                     /locus_tag="VIV63_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6124152.1"
                     /translation="MAETPENSRTRLLTATIDLVRANGYAATRVEDVCAAAGVTKGSF
                     FHHFASKEDLAIAAAAQWNDRASQLFAQAPYLSRPESLDRLLGYVSYRKELLDGEIWE
                     WSCYAGTTIQETHETHPRIRDACAASITGHVVMLGWMIDDVMREHPVEGLRAETLAIH
                     IQAVLQGAFVMAKARQDVEAARASIDHLYRYVELLFGRSTSSRKASKSQR"
     gene            complement(2328..3389)
                     /gene="leuB"
                     /locus_tag="VIV63_05850"
     CDS             complement(2328..3389)
                     /gene="leuB"
                     /locus_tag="VIV63_05850"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY6124153.1"
                     /translation="MKATIAVIAGDGIGPEVVAEGVRVLEQIGKRFGHEFLLQEYPFG
                     GIAIDLHGDPLPQTTLDACLAADAVLLGAIGGPKWGPSAAVRPEQGLLRLRRELGVYA
                     NLRPIKLHPALRDASVLKPEILDGVDLVFVRELTGGIYFGAKTRTPTLATDLCSYSAS
                     EIERITRVAGRLAMQRRKKIVSIDKSNVLETSRLWREIASHVIHSEFPEVDLEHMLVD
                     SAAMHLLKRPREFDVMLTENMFGDILTDEGAMLASSLGVLPSASIGDGSRGVYEPIHG
                     SAPDIAGKDIANPIGTILSVALLLRHSLKLEREAATVEQAVDRCIANGARTADIAEHG
                     RPAMTTRRMGDAVVAALQG"
     gene            complement(3386..3961)
                     /gene="leuD"
                     /locus_tag="VIV63_05855"
     CDS             complement(3386..3961)
                     /gene="leuD"
                     /locus_tag="VIV63_05855"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY6124154.1"
                     /translation="MAAVKTIRSRTVALPATNVDTDQIIPARFLTSTSKAGFGKHLFA
                     DWRYDTDGNPKPDFVLNKPEAKGAAILVAGRNIGCGSSREHAPWALQDFGFQAVVSTE
                     FADIFRTNSLKNGLLPVVVDEQTHTWLLGNPGVEVTIDVESTTLTLPDGTEVKFPLEG
                     FARYCLLNGVDELGFLLSQNGAISAYEQSHR"
     gene            complement(3967..5352)
                     /gene="leuC"
                     /locus_tag="VIV63_05860"
     CDS             complement(3967..5352)
                     /gene="leuC"
                     /locus_tag="VIV63_05860"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY6124155.1"
                     /translation="MFEKVWENHIVVPETADTPAVLFIDLHLTHEVTTPQAYTELRSR
                     GLKLRRPDLTLATMDHSTPTRTEQVFGGVPIAVDSAAKQVRQLEINAEEFGVELFNLK
                     DSRRGIVHIIGPELGITQPGKTIVCGDSHTSTHGAFGALAFGIGTTEVGHVLATQCLL
                     QRRPKTFAINVTGRLSAGVTAKDLILAIIGKIGVSGGTGYVLEYRGVAIEAMSMDERM
                     TICNMSIEAGARAGMIAPDATTFEYLAATPRAPKGADWDAAVAHWSKLPTDAGATFDR
                     SIEIDAAELVPMVTYGTHPGMVAPITGSVPSQGDAVFEKALKYMGLKAGEPIVNVPVN
                     NVFVGSCTNGRLSDLRNAARVLRGHKLAEGVKMLVVPGSQSVKRAAEAEGLHTVFLDA
                     GAEWRESGCSMCLGMNGDLVPTGHYSISTSNRNFEGRQGQGARTLLASPETAAASAIR
                     GRVTDPREFSN"
     gene            complement(5451..6407)
                     /locus_tag="VIV63_05865"
     CDS             complement(5451..6407)
                     /locus_tag="VIV63_05865"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY6124156.1"
                     /translation="MPIPATKYIWFNGKLVPWEKATVHVLSHALHYGSSVFEGVRAYE
                     TPRGPAIFRLRDHTKRLFDSAKIYRINLPFSEDQINHACREVISQNGLARGAYLRPVA
                     FRGYGEIGVVPKIDPPVEVAIAAWEWGKYLGAAEDEGVDVCVSSWQRVAPNTIPAMAK
                     AGGNYLSSQLIGLEAKRLGFVEGIGLSSDGTVSEGAGENLFLIKDGVLYTPGIAHSLL
                     NGITRHTVMTLAKEQGIEVREIAIPREMLYLADELFFTGTATEVAAIRSVDGIQIGNG
                     KRGAITHKLQAAYFALVQGKSEDKRGWLDYVDMNAPRAAVAS"
     gene            complement(6432..8006)
                     /locus_tag="VIV63_05870"
     CDS             complement(6432..8006)
                     /locus_tag="VIV63_05870"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY6124157.1"
                     /translation="MSHRERVMIFDTTLRDGEQSPGCSMAQPEKLRVARALADLGVDV
                     IEAGFPAASRGDWESVNAVAREVQGPIICGLARCNRDDITHVAKAIAPAANRRIHVFL
                     ATSAIHRQFKLNMAQEEIIRTAVEGVKFAREFCDDVEFSPEDASRTELDFLTQVVEAV
                     IEAGATTVNIPDTVGYTVPDEFAELFRYLRKNVRGIDKIRLSVHCHDDLGMAVANSLT
                     AVVAGARQIECTINGIGERAGNCSLEEVVMALKTRDAFFNLDTGIDTTRLYPTSRMLA
                     NIVGVPIPRNKAIVGENAFAHESGIHQHGMLKHHSTYEIMRPQDVGLSRSSLVLGKHS
                     GRHALRDRIKELGFELDDVEFARVFEEFKALADKKKELYDGDIEALVLQAEHSASGPW
                     SLVNLKTASDGSKNAEATVRLRNNGREVEESATGDGPVDAALKAIERATGVQVVLRKF
                     EVRSVSAGEDAQGEALVYVEYNQRTYRGSSVSTNIIESATQAFLEVINRIELAQGASR
                     TREERTGAARVAQAVV"
BASE COUNT         1345 a         2502 c         2713 g         1577 t
ORIGIN      
        1 ggcggtgatg gccctcgcac ggtctgacgc catgagcacg gcggcatcgg ctacctccgc
       61 catcctcggc atgcgcttga gcatcgtctt ctcggcgatg cgcgcctcga actgttcgcg
      121 cgtcacgccg gcgagccgtg catgttcgcc gatcgccgcc gacactccgg gtgcgtcggg
      181 cgatccggac gagcgcagtg ccacgacgcg gatgcccatg ccgccaagat ctaccgcgag
      241 ttgccggcac aatccttcga tggcggcgca cgcgacgccg aagccgccac tgccggtgta
      301 aggcttgcgt gccacttgcg cggtgagcgc gaggatgacg cccgaatgtc gcttcgacat
      361 gtgatgcgcc gcggcggtgg cggtgagaag gtgcgtacgc acgccgcttt caacgggctc
      421 cacgacatcc ctggctggca tcgtgagcac cgattcgcct tgcggaccac cgaagccaat
      481 gaggttgaac gaaatatcga cgcggccgcg tgccacgatg acagcgtcga gatgagcgcg
      541 tatggagtcc gggctgagtg cgtccacctt tgcggcggcc accgagccac ccgcgtgggc
      601 gatttccgtc gccaacgact gcaccttttc cagcgttcgg cccgcgagtt cgacgtgagc
      661 gccttcccgt gcaaacgcac gggccaccgc gctgcccatg gtcccggcag cgccataaac
      721 caccgcgacc ttgccttcca gcaacccggt gcgggcgccc gctgacgatc tgttgccaga
      781 tgaagcgagg gacgccatcg gtaccgcgac agcggcgcct gcggcgatga attcacggcg
      841 cgacgcggct tccatgcttc acatgcagca ggagacggga ggagtgaagc ggccgttagg
      901 cgcgacttcg ccgccttttc cgtacatgtc gtgcggacgc acccagtcgg tcatgccgcg
      961 ctcgggtgat tcgttgcgtc ccttcggtgt cacctcgaga aggcgataga tagtcatgaa
     1021 gtcttcgcca cctcgtgcga aacacgagta ggtatggaag atgttgccgg cgtcgtcgcg
     1081 gtagaacacg ttgtcgcccg acagatcctc tatgcccgga tcgcaaggct ggtagttata
     1141 gacggcacgg ccagccttca ggtcttcggg cctgaaggac acgccgaagt cgtagttgaa
     1201 gtcggagccg tacgacgaat aaaaaggcac gcgccagccc atgcgctcgc gcagcgccgt
     1261 gatctccggc atggttgcgc gcgctacggc gacgtatgac acgtcgtgat tgtcgagatg
     1321 cggcaacagg ccttcgatgt gatcgatgcc caaagcgcag cccacacatt gcagcggctg
     1381 gccgggcccc tgcatgacgc tttgaaccca gagttgcgag cggccgcgga acagatcggc
     1441 gaacctgacc cggccttccg gcgagtcgaa ctcgtagtcc ttctcgatct tgacccacgg
     1501 catcgcgcga cgcttttcgg cgagcgcgtc gcgacggcgc gtgaactcct tttcctccgc
     1561 catgaggcgc ttgcgttcga cgatccactc ttgctggctc acgacccggt tcatggcgcc
     1621 tccttcagcg ttgcgatttt gatgccttgc gactactggt cgaacggccg aacagcaatt
     1681 cgacataccg atacaggtga tcgatgcttg cgcgcgcggc ttcgacgtcc tggcgcgcct
     1741 tggccatcac aaaagcaccc tggagcacgg cctggatgtg gatcgcgagt gtttcggcgc
     1801 gaagaccttc cacggggtgc tcgcgcatca cgtcgtcgat catccagccc agcatgacga
     1861 cgtggccggt gatcgaggcg gcgcaggcgt cgcgaattcg cggatgggtc tcgtgcgttt
     1921 cctggatggt cgtgcccgcg tagcagctcc attcccagat ctcgccgtcg agcagttcct
     1981 ttcggtagct cacgtagccg agcaggcgat cgagcgattc aggtctggag aggtagggcg
     2041 cctgcgcgaa gagttgcgac gcgcgatcgt tccactgcgc cgccgctgcg atggcgagat
     2101 cttccttgct ggcgaagtgg tggaagaagc tgcccttggt gacgccggcg gccgcgcaga
     2161 catcttccac gcgtgtggcg gcgtaaccgt tggcgcgcac caggtcgatg gtggcggtca
     2221 gcagtcgcgt gcgggaattc tcgggagttt cggccacggc atcttccgta ccagcaggta
     2281 tggcggacca taccggccgg tacgtaggcc gtcaagagcc gggcgggtca gccctgcagc
     2341 gctgcgacca ccgcgtcgcc catgcggcgc gtggtcatcg ccggccgtcc gtgttcggcg
     2401 atgtccgcgg tccgcgcacc gttggcgatg cagcgatcga cagcctgttc gaccgtcgcc
     2461 gcctcgcgtt cgagcttgag cgaatgccgc agcagcagcg cgacgctcag gatggtgccg
     2521 atcgggttgg cgatgtcttt gccggcgatg tcaggcgcgg atccgtggat gggctcgtag
     2581 acgccgcggc tgccatcgcc gatggatgcc gacggcaaca cgccgagcga gctcgcgagc
     2641 atggcgcctt cgtcggtcag gatgtcgccg aacatgtttt cggtgagcat gacgtcgaat
     2701 tcgcgcgggc gtttcagcag gtgcatggcg gcggaatcca ccagcatgtg ttcgagatcc
     2761 acttcgggaa actccgagtg gatgacgtgc gaggcgattt cgcgccacag gcgcgaggtc
     2821 tcgagcacgt tcgatttgtc gatggagacg atcttcttgc ggcgctgcat ggcgagccgg
     2881 ccggccacgc gcgtgatgcg ttcgatctcg ctggcggagt agctgcacag atccgtggca
     2941 agtgtcgggg tgcgcgtctt ggcgccgaag tagatgccac cggtgagttc gcgcacgaac
     3001 accaggtcca ccccgtcgag gatttccggc ttgagcacgg acgcatcgcg gagggcaggg
     3061 tgaagcttga tcgggcgcag gttggcgtat acgccgagtt cgcggcgcag ccggagcaag
     3121 ccctgctcgg ggcgcacggc ggccgacggt ccccatttcg ggccgccgat ggcgcctaac
     3181 aacaccgcat cggccgccag gcaggcgtcg agcgtggttt gcggcagcgg gtcgccgtgc
     3241 agatcgatgg cgatgccacc gaagggatac tcctggagca ggaattcgtg tccgaagcgt
     3301 ttgccgatct gctcgagcac gcgtacgcct tcggcgacga cttccggccc gatgccatcg
     3361 ccggcgatga ccgcaattgt tgctttcatc ggtgactttg ctcgtatgcg ctgatggcgc
     3421 cattctgcga gaggaggaag ccgagctcat cgacgccgtt caacaggcag taacgcgcga
     3481 aaccttcgag tggaaatttc acctcggtgc catcgggcag cgtgagcgtg gtgctttcaa
     3541 cgtcgatcgt cacttccacg cccggatttc ccagcagcca cgtgtgcgtc tgctcgtcga
     3601 caacgaccgg cagcaagccg ttcttgagac tgttggtgcg gaagatgtcg gcgaactcgg
     3661 tgctgacgac ggcctggaaa ccgaaatcct gcagcgccca cggagcatgt tcgcgtgagg
     3721 atccgcagcc gatgttgcgg ccggcgacga ggatggcggc gcctttcgcc tcgggcttgt
     3781 tgagcacgaa atcgggtttc ggatttccgt cggtgtcgta gcgccagtcg gcgaacaggt
     3841 gtttgccgaa accggctttc gacgtggagg tgagaaaccg cgccggaatg atctggtcgg
     3901 tgtcgacatt ggtcgcgggc agggcgaccg tgcgtgagcg aatggttttt acggcagcca
     3961 tgtttttcag ttcgagaatt cgcgcggatc ggtgacgcgg ccacggatgg cggacgcggc
     4021 agcggtttcg ggactggcga gcagggtgcg cgcaccctgg ccctggcgtc cttcgaagtt
     4081 ccggttgctg gtgctgatgg agtagtgccc ggtgggcacg aggtcgccgt tcatgccgag
     4141 gcacatcgag caaccgctct cgcgccactc ggcgcccgca tccagaaaca cggtgtgcag
     4201 gccttcggct tcagcggcgc gtttgacgga ttgcgagccg ggcacgacga gcatctttac
     4261 gccctcggcc agcttgtgac cgcgcagcac gcgcgcggcg ttgcgcagat ccgagagacg
     4321 gccattggtg cagctgccga cgaacacgtt gttcaccgga acgttgacga tcggttcgcc
     4381 cgccttgaga cccatgtact tcagcgcttt ttcgaacacg gcatcgccct gcgagggaac
     4441 ggagccggtg atgggcgcca ccattccggg atgggtgccg taggtgacca tgggaacgag
     4501 ttccgccgcg tctatctcga tggagcggtc gaaggtggcg ccggcatcgg tgggtagttt
     4561 gctccagtgc gcgaccgccg cgtcccagtc cgcgcccttg ggcgcccgcg gcgtggccgc
     4621 gagatattcg aacgtggtgg cgtccggcgc gatcatgccc gcgcgcgctc cggcctcgat
     4681 ggacatgttg cagatggtca tgcgctcgtc catcgacatg gcttcgatgg ccacgccgcg
     4741 atattcgagc acgtaaccgg tgccgcccga tacgccgatc ttgccgatga tggcgagaat
     4801 gaggtctttg gcggtgacgc ccgcggaaag cctgccggtg acattgatgg cgaaggtttt
     4861 cggcctgcgc tgcaggagac attgcgtggc cagcacgtgg ccgacctcgg tggtgccgat
     4921 gccaaaggcg agggcgccga acgcgccgtg cgtggaggtg tgtgagtctc cgcagacaat
     4981 ggtcttgccc ggctgcgtga tgccgagttc gggcccgatg atgtgcacga tgccgcggcg
     5041 gctgtccttg agattgaaca actcgacgcc gaattcttcg gcattgatct cgagttgtct
     5101 cacctgcttc gccgccgaat ccaccgcgat gggcacgccg ccgaacacct gttcggtgcg
     5161 cgtgggcgtc gagtgatcca tggtggcgag cgtgaggtcc ggacggcgca gcttcaggcc
     5221 gcgcgagcgc agttccgtgt acgcctgcgg cgtcgtcact tcatgcgtga gatgcagatc
     5281 gatgaacagg acggcggggg tatcggcggt ctcgggaacc acgatgtggt tttcccagac
     5341 tttttcgaac atcgttttgg gttggctatt cacagagtcg tcttggtgaa gaatggggtc
     5401 aggcaccatt ttcttgaaga caagaaatat ggagccagac ccctgactaa ctagcttgct
     5461 actgcggctc tcggcgcgtt catatcgacg taatcgagcc agccacgctt gtcttccgac
     5521 ttgccctgca ccagcgcgaa atacgcggcc tgcagcttgt gcgtgatggc tccacgcttg
     5581 ccgttgccga tctggatgcc gtccaccgag cggatcgccg ccacttcggt ggcggtgccg
     5641 gtgaagaaca gttcgtcggc gaggtagagc atctcgcgcg ggatggcgat ctcgcggact
     5701 tcgatgccct gttccttcgc gagcgtcatg acggtgtgac gcgtgatgcc gttgagcagc
     5761 gagtgggcga tgcccggcgt gtagagcacg ccatccttga tgaggaacag attctcgccg
     5821 gcgccttcgc tgacggttcc gtcggaagac aggccgatgc cctcgacgaa gcccaggcgc
     5881 ttggcctcga gcccgatgag ctggctggac aggtaattgc cgccggcctt ggccatggcg
     5941 ggaatggtat tcggcgcgac gcgctgccag gacgacacgc agacatcgac gccttcgtct
     6001 tccgcagcgc cgaggtactt gccccattcc caggcggcga tcgccacttc cacgggcggg
     6061 tcgatcttcg gcaccacgcc gatttcaccg tagccgcgga acgccacggg gcgcaggtac
     6121 gcaccacgcg ccaggccgtt ctgcgaaatg acttcgcggc aggcgtgatt gatctggtct
     6181 tcggagaagg gcaggttgat ccggtagatc ttcgccgaat cgaacaggcg tttcgtgtgg
     6241 tcgcggaggc ggaagatggc cggaccgcgc ggggtctcgt aggcgcgtac gccttcgaac
     6301 accgacgagc cgtagtgcag cgcatgcgag agcacgtgca ccgtggcctt ttcccagggc
     6361 acgagtttgc cgttgaacca gatgtatttc gttgcgggta tgggcatgag aagactccga
     6421 gaacctttgg tctacaccac ggcctgcgca acacgcgcgg cgccagtgcg ctcttcgcgc
     6481 gtgcgggatg cgccctgcgc gagttcgatg cgattgatca cttcgaggaa ggcctgtgtt
     6541 gcggactcga tgatgttggt actgacgctc gagccgcgat aagtgcgctg attgtactcc
     6601 acgtacacca gtgcttcgcc ttgcgcgtct tcaccggccg atacgctgcg cacttcgaac
     6661 ttgcgaagca ctacctgcac tcccgtggcg cgttcgatcg ccttgagcgc ggcgtcgacc
     6721 ggaccgtcgc cggtggcgct ctcttcgact tcgcggccgt tgttgcgcag gcgtaccgtc
     6781 gcttcggcat tcttgcttcc atctgaagcg gtcttgaggt tgacgagcga ccaggggccc
     6841 gaagcactgt gttcggcctg gagcacgaga gcttcgatgt cgccgtcgta gagttccttc
     6901 ttcttgtccg ccagcgcctt gaattcctcg aacacgcgcg cgaattccac gtcgtcgagt
     6961 tcgaaaccga gttccttgat gcggtctcgc agcgcatggc ggccgctgtg tttgcccagc
     7021 acgagcgagg agcgcgacag acccacgtcc tgcgggcgca tgatctcgta cgtcgagtga
     7081 tgtttgagca tgccgtgctg gtgaatgccc gattcgtgcg cgaacgcgtt ctcgccgacg
     7141 atggccttgt tgcgcggaat gggcacgccg acgatgttcg ccagcatgcg tgaagtgggg
     7201 tagaggcggg tggtgtcgat gcccgtgtcg agattgaaga atgcatcgcg cgtcttgagc
     7261 gccatgacga cttcttccag cgagcaatta ccggcgcgtt cgccgatgcc gttgatggtg
     7321 cattcgatct gccgcgcacc ggccacgacc gcggtgagac tgttggccac cgccatgccg
     7381 agatcgtcgt ggcagtggac cgacaggcgg atcttgtcga tgccgcgcac gttcttgcgc
     7441 aggtagcgga acagctccgc gaactcatcg ggcacggtgt agcccacggt gtcgggaatg
     7501 ttgaccgtgg tcgctccggc ctcgatcacc gcttcgacga cctgcgtcag gaagtcgagc
     7561 tccgtgcgcg aggcgtcttc gggcgagaac tccacgtcgt cgcagaattc acgcgcgaac
     7621 ttcacgcctt cgaccgcggt gcggatgatt tcttcctgcg ccatgttcag cttgaactgg
     7681 cgatgaatgg cgctggtcgc gaggaacacg tggatgcggc gattcgccgc cggcgcgatc
     7741 gccttggcca cgtgcgtgat gtcgtcgcgg ttgcagcgcg cgaggccgca gatgataggt
     7801 ccctgtacct cgcgcgccac cgcattcacg ctttcccagt cgccgcgcga ggcggcgggg
     7861 aaacccgctt cgatcacgtc gaccccaagg tccgcaagcg cacgcgccac ccgaagtttt
     7921 tcgggctgcg ccatgctgca ccccggcgat tgctcgccgt cgcgcagcgt cgtgtcgaag
     7981 atcatgactc gttcacgatg ggacatgggg gtctacctcc gtcagataag ccgcgggttg
     8041 ttagccgcga ccctgttcca gccacggcat gcgggcgcgc agttccttgc ccacggtctc
     8101 gatctgatga tcgagatccg cctgcatgag gcggttg
//
LOCUS       DATDEU010000237         5008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2083594, whole genome shotgun sequence.
ACCESSION   DATDEU010000237 DATDEU010000000
VERSION     DATDEU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083594"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VIV63_05875"
     CDS             <1..554
                     /locus_tag="VIV63_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HEY6124158.1"
                     /translation="SVPSAQQTPPVPAVDVSPNYIVGPGDTLQIFVWRNPELSTTVPV
                     RPDGKITTPLVQDMVAVGKTPSELARDIEKVLQEYIRSPQVNIIVSNPVSAFSQIKVV
                     GQVQSPQSMAFREGMTVLDAVLASGGLGPYAAGNRAKVVRQVNGKQTEIKIKLNDLVN
                     KGAMKHNIKLQPGDVLVVPESRL"
     gene            562..2169
                     /locus_tag="VIV63_05880"
     CDS             562..2169
                     /locus_tag="VIV63_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HEY6124159.1"
                     /translation="MHPVVEKISQEARGMWRFRRYALITAWAVCLLGWFVVFIIPDTF
                     EARARVNVDTKTPLTPLLRDIAVDSDVPTQLNIVRQALLGATTLDGVAHKLGLDGAAQ
                     SAGNKQQLLASLSSRLEIALEPPATRDPRVPNTFYRITFRDGNRDTAIAVVDALLNEF
                     VEKTMGSEYTGTQKAQRFFETKVKEYGEQLSELEAQLADFKKKNMGLVPGEEGDYFQR
                     LTATRTEIDRIEAQLSIATSRRAELQRQLRGETPFVPTSSAGSSTRGGAAGGSSDTAS
                     RIAETETRLNELLLRFTDKHPDVLATRETLEQLKARQTEEIAALRRGDAGAAAASGAA
                     SNPVFQNIQLQANQTDVEIAALRVQLAERKRIEGDLRRVADTAPEVEAEYQSLTRNYT
                     VTQSQYKSMIERLERARLSEDAAETGVIRFDIVDPPTAALKPIFPNRTLLLAVVLLGG
                     IVVGLGVAWLMHMLRPVFSSARSITELTGLPVLGSVSRTWLEKQKAALRNGFLRYSAL
                     AGLLFVLFIVVEVIKNPASRYLRELLG"
     gene            2179..3093
                     /locus_tag="VIV63_05885"
     CDS             2179..3093
                     /locus_tag="VIV63_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HEY6124160.1"
                     /translation="MSLVEQALKKLQQARDAGQPARQPEHHQPTLSSSARDPLAQTMP
                     TRIVVPQQSLTVNTSALRAAGFLAPDSQERRIANEFRNIKRPLIAQALGRGGQKIQNG
                     RLIMVTSSLPGDGKTFTSLNLALSMAQEMDLSVLLVDSDVAKRHISRMLGVEGERGLM
                     DVLRDETADIDAVVHGTDIPGLNVLPAGKPSENATELLASPRMEQVIKHLGSRADSRI
                     VLFDSPPLLLTSESRALAASVGQIVLVVCAGVTPQRAVFDSLDILGEGKAVSVVLNQA
                     DEGRSAEYGYSYYGDTAPEERGSPGARE"
     gene            3099..4418
                     /locus_tag="VIV63_05890"
     CDS             3099..4418
                     /locus_tag="VIV63_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124161.1"
                     /translation="MSAVSKARFITAALVGVIAALHAAGAQAQVEYGFTAGYGHSDNV
                     FRLSDDEITSDIATAGVELRWEEDRTRFDADVRADLDFNHYLNVDDNFDVDDQVTGNA
                     NGQLTFGIIPERFTWLLEDTFGQTQQDPLVPATPESLETTNYLTTGPDLIFVLGSTNS
                     LRFYGRYSLTTYEISPFDNTRAGGGVTFQRQMSGRSSLAFNVDSDDVDYDDEGNVDFK
                     RSSASATYELDASRTDISARLGYTQMDLDDGSEKTGPLVDVSVRRQVSNSSLLNLRFG
                     TQLSDAAEALRTSIEADEDFGGDGSGVVATSSTFENQFVSLAWEFDRPRTSFEVSAGY
                     DKDVYESGTDLDRERLLFEAGAERHLNNRLSLRARASYDEETYELDGSVIEELRFILG
                     GSYRFGRDTGMELWGERLKRDSNTTSGGGHSVENRIFLTLFYRPAAR"
     gene            4415..>5008
                     /locus_tag="VIV63_05895"
     CDS             4415..>5008
                     /locus_tag="VIV63_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6124162.1"
                     /translation="MNAAAATVNAMTVDVEDYFHVSALAEVIDRKDWDRMEYRAEQST
                     ERLLTLFAEKGIKATFFVLGWVTKRSPNLVRKIHAAGHEVACHGLTHELVYRQTPEVF
                     RDETRVSKAMLEDTIGAPVLGYRAASYSITRESLWAIDILCDLGFRYDSSIFPIAHDR
                     YGIPGAETRPGIMKDSRGNGIVEFPLSTVKFMGRRLPC"
BASE COUNT          941 a         1726 c         1514 g          827 t
ORIGIN      
        1 tctcggttcc gtctgcccag cagacgccgc cggttccggc agtcgacgtc agccccaact
       61 acatcgtcgg ccccggcgac acgctgcaga tcttcgtctg gcgcaacccc gagctcagca
      121 ccacggttcc ggtacgcccc gacggcaaga tcaccacgcc gctggttcag gacatggtgg
      181 ccgtcggcaa gacgccatcg gaactggcgc gcgacatcga gaaggtgctc caggaataca
      241 tccgttcacc gcaggtgaac atcatcgtca gcaatccggt gagcgcgttc agccagatca
      301 aggtggtcgg ccaggtgcag tcgccgcagt cgatggcgtt tcgcgaaggc atgaccgtgc
      361 tcgacgccgt gctcgcgtcc ggcggactcg gtccttacgc cgcgggcaac cgcgccaagg
      421 tcgtgcgcca ggtcaacggc aagcagaccg agatcaagat caagctcaac gacctggtga
      481 acaagggcgc catgaaacac aacatcaagc tgcagcccgg cgacgtgttg gtcgtccccg
      541 aatcgcggct ctgagaaacc tatgcatcca gtcgtagaaa agatttcgca ggaagcgcgc
      601 ggcatgtggc gcttccgccg gtacgcgctc atcaccgcgt gggccgtctg cctgctcgga
      661 tggttcgtgg tgttcatcat cccggatacc ttcgaagccc gcgcgcgcgt caacgtcgac
      721 accaagacgc cgctgacgcc gctgctgcgt gacatcgccg tcgacagcga cgtgccgacg
      781 cagctcaaca tcgtccgcca ggccttgtta ggcgcgacca cgctcgacgg cgtggcgcac
      841 aagctgggtc tcgacggcgc cgcgcagtcg gccggcaaca agcagcagct tctggccagt
      901 ctctcgtcgc gcctggaaat cgcgctcgag ccgccggcca cgcgcgatcc gcgcgtaccg
      961 aacactttct atcgcatcac tttccgcgac ggcaatcgcg acacggccat tgccgtcgtc
     1021 gatgcgctgc tcaacgaatt cgtcgagaag acgatgggtt cggagtacac gggcacgcag
     1081 aaggcgcagc gtttcttcga aaccaaggtg aaggaatacg gcgagcagct gagcgaactc
     1141 gaagcccagc tcgcggactt caagaagaag aacatgggcc tggtgccggg cgaagagggc
     1201 gactacttcc agcgcctgac ggccacgcgt accgagatcg accgcatcga agcgcagctt
     1261 tcgatcgcca ccagccgtcg cgcggaactg cagcgtcagc tgcgcggcga aacaccgttc
     1321 gtcccgacgt cctccgcggg ttccagtact cgcggtggtg cggccggcgg gtcttcggac
     1381 acggcgtcgc gcatcgcgga aaccgaaacg cggctcaatg aactgctgct gcgcttcacc
     1441 gacaaacacc cggatgtcct cgcgactcgc gagacgctcg agcagctcaa ggctcgccag
     1501 acggaagaga tcgcggcgct tcgccgcggc gatgcgggcg cggccgccgc gtccggcgcc
     1561 gccagcaatc cggtgttcca gaacatccag ctccaggcca accagacgga cgtggaaatc
     1621 gccgccttgc gcgtgcagct cgccgagcgc aagcgcatcg aaggcgacct gcgccgcgtc
     1681 gccgacacgg cgcccgaagt ggaagccgaa taccagagtc tgacgcgcaa ctacacggtc
     1741 acgcagtcgc agtacaagtc gatgatcgag cgtctcgaac gcgcgcgtct gtcggaagac
     1801 gcggccgaaa cgggcgtcat ccgtttcgac atcgtcgacc cgcccaccgc cgcgctcaag
     1861 ccgatcttcc cgaaccgcac gctgctgctc gcggtcgtgt tgttaggcgg cattgtcgtc
     1921 ggtctcggag tcgcctggct catgcacatg ctgcgcccgg tgttctcgag cgcacgctcg
     1981 atcacggaac tcaccggtct cccggtgctc ggttcggtga gccgcacctg gctcgagaag
     2041 cagaaggccg ccttgcgaaa tggattcctg cgctacagcg cccttgcggg cctgctgttc
     2101 gtattgttta tcgtggtgga agtcatcaag aaccccgcct cgcgctacct gcgcgaactt
     2161 ctgggctagg agcgccgaat gagtctcgtc gaacaagcct tgaagaaact ccagcaggcg
     2221 cgtgacgccg gccagccggc gcgtcagcca gagcaccacc agccgacgtt gtccagcagc
     2281 gcgcgcgatc cgctcgcaca gaccatgccg acccgcatcg tggtgccgca gcagtcgctg
     2341 accgtcaaca ccagcgcctt gcgcgccgca ggtttcctgg cgcccgattc ccaggaacgg
     2401 cgcatcgcca acgaattccg caacatcaag cgtccgctca tcgcgcaggc gctgggccgt
     2461 ggcggccaga agatccagaa cggccgtctg atcatggtga ccagttcgct gcccggcgac
     2521 ggcaagacct tcaccagcct caatctcgca ctgtccatgg cccaggaaat ggacctgagc
     2581 gtgctgctgg tcgactccga cgtggcgaag cggcacatca gccgcatgct cggcgtcgaa
     2641 ggcgagcgcg gtctcatgga cgtgctgcgc gacgagaccg ccgacatcga tgccgtcgtt
     2701 cacggcaccg acattcccgg tctcaacgtg ttgccggccg gcaagccttc ggagaacgcc
     2761 accgaactcc tcgccagtcc gcgcatggaa caggtcatca aacacctggg ttcgcgcgcc
     2821 gattcgcgca tcgtgctgtt cgactcgccg ccgctgctgc tcaccagcga atcacgtgcg
     2881 ctggccgcgt cggtcggtca gatcgtgctc gtcgtctgcg ccggcgtgac gccgcagcgc
     2941 gccgtgttcg actcgctcga catcctgggc gaaggcaagg cggtctccgt cgtgctgaac
     3001 caggccgacg aaggacgcag cgccgaatac ggttattcct actacggcga caccgcgccg
     3061 gaagaacgcg gttctccggg cgcgcgcgag tgaggcagat gtccgcggtc agcaaagcac
     3121 gattcatcac cgccgctctc gtgggcgtca tcgccgcact acacgcggcc ggcgcacagg
     3181 cgcaggtgga atacggcttc accgcgggtt acggccactc cgacaacgtc tttcgactct
     3241 cggacgacga gatcacctcc gacatcgcga ccgccggggt cgagctgcgg tgggaagaag
     3301 accgcacgcg cttcgacgcg gacgttcgcg ccgacctcga cttcaatcac tacctgaacg
     3361 tcgacgacaa cttcgacgtc gacgaccagg tcacgggcaa cgccaacggc cagctgacct
     3421 tcggcatcat tcccgagcgc ttcacctggc tgctcgaaga cactttcggc cagacccagc
     3481 aggacccgct ggtaccggcg acccccgagt cgctcgagac cactaactac ctgaccaccg
     3541 gccccgatct catcttcgtg ctcggctcca ccaactcgtt gcggttctac ggccgctact
     3601 cgctgaccac ttacgagatc agccctttcg acaacacgcg tgccggcggc ggtgtgacgt
     3661 tccagcggca gatgtccgga cgcagttcgc tcgcgttcaa cgtggattcc gacgacgtcg
     3721 actacgacga cgaaggcaac gtcgatttca agcgcagcag tgccagcgcc acgtacgaac
     3781 tcgacgcgtc ccgcaccgac atcagcgccc ggctcggtta tacgcagatg gacctcgacg
     3841 acggctcgga aaagaccggc cccctcgtgg atgtctcggt gcgtcgccag gtctccaatt
     3901 cctcgttgct caacctccgc ttcggcacgc agctcagtga tgccgccgaa gcgctgcgca
     3961 ccagcatcga agcggatgaa gacttcggcg gcgatggttc cggtgtcgtc gccacgtcga
     4021 gcaccttcga aaaccagttc gtttcgctgg cctgggaatt cgatcgcccg cgaacctcgt
     4081 tcgaagtctc cgcgggctac gacaaggacg tgtacgaatc cggtaccgat ctcgatcgcg
     4141 aacgcctgct gttcgaggct ggcgctgagc ggcacctcaa caaccggctg tcgctgcgcg
     4201 cgcgcgcctc ctacgacgag gaaacgtacg aactcgacgg cagcgtcatc gaggaactgc
     4261 gattcatcct cggcggttcc tatcgcttcg gccgcgacac cggcatggaa ttgtggggcg
     4321 agcgcctgaa gcgcgattcc aacaccactt cgggtggtgg ccattcggtc gaaaaccgga
     4381 tcttcctgac tctcttctac cgtcccgcgg cgcgatgaac gccgccgccg cgaccgtcaa
     4441 cgccatgacc gtggacgtgg aagactactt ccacgtatcc gcgctggccg aggtcatcga
     4501 tcgcaaggac tgggaccgca tggaataccg tgcggagcag agcaccgagc ggttgctcac
     4561 gttgtttgcc gagaagggca tcaaggccac gtttttcgtg ctcggctggg tcacgaagag
     4621 aagtccgaac ctcgtccgca agatccacgc cgccggtcac gaagtggcct gccacggtct
     4681 cacgcacgaa ctcgtctacc ggcagactcc cgaagtgttt cgcgatgaga cgcgcgtttc
     4741 gaaggccatg ctcgaggaca ccatcggcgc gccggtgctc ggctaccgcg cggccagcta
     4801 ctccattacg cgcgaatcgc tgtgggccat cgacatcctc tgcgatctcg gcttccgcta
     4861 cgactccagc atcttcccga tcgcgcacga ccggtacggc attcccggcg ccgagacgcg
     4921 ccccggcatc atgaaagaca gccgcggcaa cggcatcgtc gagttcccgc tatccaccgt
     4981 gaagttcatg ggccgccggc tgccgtgt
//
LOCUS       DATDEU010000238         4356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1937251, whole genome shotgun sequence.
ACCESSION   DATDEU010000238 DATDEU010000000
VERSION     DATDEU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937251"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VIV63_05900"
     CDS             complement(<1..588)
                     /locus_tag="VIV63_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6124163.1"
                     /translation="MLSIQGLNQFYSGSHILWDVKLEVPEGSCTCLMGRNGMGKTTLL
                     KCIMGLLPIASGAMSYGLGAESTDLSKIAADRRARLGIAYVPQGREIFSQLTVEENLR
                     VGFGARRGNKINEVPEHIFEWFPVLKKMLRRRGGDLSGGQQQQLAIGRAMVLDPKLLI
                     LDEPAEGIQPNIVAEIGDIIMRLNREAKLTVLLVEQ"
     gene            complement(595..1434)
                     /gene="urtD"
                     /locus_tag="VIV63_05905"
     CDS             complement(595..1434)
                     /gene="urtD"
                     /locus_tag="VIV63_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEY6124164.1"
                     /translation="MNAAQAVKQGWQKVFGAGTGSDRPMPGDASHGTILYVEDITVSF
                     DGFRALNNLTLYIDVGELRCIIGPNGAGKTTMMDVITGKTRPDAGSAWFGITTDLLTL
                     TEPEIAEAGIGRKFQKPTVFEQHSVLQNLALAAAGNKSVWHTLFKPIARTQLARIDEV
                     LVTVGLKDQRDLRAGILSHGQKQWLEIGMLLMQDPRLLLVDEPVAGMTPQEVERTAEL
                     LLSLAGKHSVVVVEHDMEFVRSIARTVTVLHEGSVLAEGSMDAVQNDPKVVEVYLGEP
                     QGK"
     gene            complement(1431..2564)
                     /gene="urtC"
                     /locus_tag="VIV63_05910"
     CDS             complement(1431..2564)
                     /gene="urtC"
                     /locus_tag="VIV63_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEY6124165.1"
                     /translation="MTRGPLLNLLVGERGTAVFLVIVAVVGVIVPVLHLVPAEGSALQ
                     LSGYTMTLIGKYMTYALLAVAVDLVWGYCGILSLGHAAFFALGGYAMGMYLMRQIGTR
                     GVYGDPVLPDFMVFLNYKELPWFWHGFDMFWFAMLMVAVVPGLLALIFGWLAFRSRVT
                     GVYLSIITQALTYALMLAFFRNDMGFGGNNGMTDFKDILGFAINTPTTRVALFVASAI
                     ALIVGYLACRYITLSRAGRVVQAIRDAESRTRFIGYRVESYKLWIFTFSAVLAGIAGA
                     LYVPQVGIINPSEFQPINSIEVVIWVAVGGRGMLYGAAAGAVIVNYAKTFFTAALPEF
                     WLYALGLLFVVVTLFLPRGFVGLLDRFGRKPPASPPEAKAVPA"
     gene            complement(2566..4212)
                     /gene="urtB"
                     /locus_tag="VIV63_05915"
     CDS             complement(2566..4212)
                     /gene="urtB"
                     /locus_tag="VIV63_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEY6124166.1"
                     /translation="MFLRRFGYVAVFTLIAVLFAAAVRAQTTAPAPGDAFASAVAGLA
                     AESFPEKSAAVSTLTELKHPNGRVVLAALLDGKLYVRTSDTKVFITDGGETQLALTDP
                     LTLAASGSGNPEEFSRITTNNSLRKTLRSAVASFALSDPDAGVRLAAVREMLRSIDEE
                     SVGMLRASVEKESDDDVRKAMREGLALADLDSTEADTRLSAVQALKDSLSPDVYNRLT
                     TMVTAVDGAFPEPDERVRTTAREVIASIDSSRRVYSTIETLFFGLSLGSVLVLAAIGL
                     AITFGVMGVINMAHGELMMLGAYTTYVVQLAMPNHIGMSIFVAIPLAFLVSACVGMLI
                     ERGVVRFLYGRPLETLLATFGVSLILQQVVRDVFSANNRPVETPGWMAGSMQINEALS
                     ITYNRLYVILFMIVVFVILQLVLQKTSLGLKVRAVAQNRNMARAMGVRTQWVDAMTFG
                     LGSGIAGVAGVALSQLTNVGPNLGQQYIIDSFMVVVFGGVGNLWGTLIGGMSLGVVNK
                     VLEPWAGAVLAKILVLIALILFIQRRPRGLFPQKGRAAEN"
BASE COUNT          842 a         1419 c         1330 g          765 t
ORIGIN      
        1 ctgttccact aacagcacgg tgagtttcgc ctcgcggttg aggcgcatga tgatatcgcc
       61 gatctcggcc acgatgttgg gctgaatgcc ctccgccggt tcatcgagga tcagcagctt
      121 cggatcgagc accatggccc gcccgatggc cagctgctgc tgctgcccgc ccgacaggtc
      181 gccgccgcgg cgcctcagca ttttcttgag cacgggaaac cattcgaaga tgtgttccgg
      241 cacctcgttg atcttgttgc cgcggcgcgc tccgaatccg acgcgcagat tctcctcgac
      301 ggtgagctgc gagaagatct cgcggccttg cggcacatac gcgatgccga gccgcgcccg
      361 ccggtccgcc gcgatcttcg acagatcggt gctctcagcg ccaagtccgt agctcatggc
      421 gcccgatgcg atcggcagca gacccatgat gcatttcagc agcgtggtct tgcccatgcc
      481 gtttcggccc atgaggcacg tgcacgagcc ctcgggcact tcgagtttca catcccagag
      541 gatatggctg ccgctgtaaa actggttgag accctggatg gacagcatgg ccggctactt
      601 cccctgcggc tcgccgagat acacctcgac gaccttcgga tcattctgca ccgcgtccat
      661 gctgccttcg gcgagcaccg agccttcgtg caacaccgtg accgtgcgcg caatcgaacg
      721 cacgaactcc atgtcgtgtt cgaccaccac caccgaatgt ttgccggcca gcgacagcag
      781 cagctccgcc gtgcgctcga cttcctgcgg cgtcatgccc gccaccggct cgtcgaccag
      841 cagcaaccga gggtcctgca tgagcaacat gccgatctcc agccactgct tctggccgtg
      901 cgagagaatt cccgcgcgaa gatcgcgctg atctttcagc ccgaccgtga ccagcacctc
      961 atcgatgcgt gcgagctgcg ttcgtgcaat cggcttgaac agcgtgtgcc agaccgactt
     1021 gtttcccgcg gccgcgagcg ccaggttttg caggacactg tgctgctcga aaaccgtcgg
     1081 cttctgaaat ttccgtccaa ttccggcctc cgcgatttca ggttcagtga gcgtaagcag
     1141 atccgtggtg atgccgaacc aggcggagcc cgcatcgggc cgcgtcttgc cggtgatcac
     1201 gtccatcatc gtcgtcttgc ccgcgccgtt cgggccgatg atgcagcgca gttcgcccac
     1261 atcgatgtac agcgtgagat tgttgagcgc gcggaaacca tcgaagctca cggtgatgtc
     1321 ttcgacgtaa aggatcgtgc cgtgcgaggc gtcgcccggc atgggccggt cactgcccgt
     1381 gccggcgccg aacaccttct gccaaccttg tttgacggcc tgtgcggcgt tcatgcgggc
     1441 accgctttgg cttccggcgg actcgccggc ggttttctgc cgaaccggtc gagcaggccc
     1501 acgaatccgc gcggcaggaa cagcgtcacc acgacgaaca acaagccgag cgcgtagagc
     1561 cagaactccg gcaacgccgc ggtgaagaat gtcttcgcgt agttcacgat gacggcgccc
     1621 gctgcggcgc cgtacaacat tccgcggccg ccgaccgcca cccagatcac cacttcaatg
     1681 gagttgatgg gctggaactc gctggggttg atgatgccga cctgtggcac atacagcgcg
     1741 ccggcgattc cggccagcac cgccgagaat gtgaagatcc acagcttgta cgactccacg
     1801 cggtagccga tgaatcgcgt gcggctttcc gcgtcgcgga tggcctgcac cacgcgcccg
     1861 gcgcgggaca gcgtgatgta gcggcacgcg agatatccca cgatcagcgc gatggcgctg
     1921 gccacgaaca gcgccacgcg cgtcgtcggc gtgttgatgg cgaaccccag gatgtccttg
     1981 aagtccgtca tgccgttgtt gccgccgaaa cccatgtcgt tgcggaagaa tgcgagcatc
     2041 agcgcatagg tcagcgcctg cgtgatgatg gatagataga caccggtcac gcgcgagcgg
     2101 aacgccagcc agccgaaaat gagcgcgagc agtcccggaa ccacggccac catgagcatg
     2161 gcaaaccaga acatgtcgaa gccgtgccag aaccacggca gttccttgta gttcaggaac
     2221 accatgaaat ccggcagcac cggatcgcca tacacgccgc gcgtgccgat ctggcgcatc
     2281 aggtacatgc ccatggcgta accgccgagc gcgaagaacg ccgcgtgtcc cagcgacagg
     2341 atgccgcaat agccccagac cagatccacg gccacggcga gcagcgcgta cgtcatgtac
     2401 ttgccgatca gcgtcatggt gtagcccgag agctgcagcg ccgagccttc ggccgggacg
     2461 agatgcagca ccggcacgat gacgcccacc accgcgacga tcacgaggaa cacggcggtg
     2521 ccgcgctcgc cgaccaacag gttgagaaga ggtccacgcg tcatttcaat tctccgccgc
     2581 ccggcccttc tgcgggaaca acccgcgcgg ccgtcgctgg atgaagagga tgagcgcgat
     2641 cagcacgagg atcttcgcca gcaccgcgcc ggcccagggc tccagcacct tgttgaccac
     2701 gcccagcgac atgccgccga tcagcgtgcc ccagagattg ccgacgccac cgaacaccac
     2761 caccatgaac gagtcgatga tgtattgctg gccgagattg ggacccacgt tggtgagctg
     2821 actgagcgcg accccggcga cgccggcgat gcccgagccg agcccgaacg tcatggcgtc
     2881 cacccactga gtacgcacgc ccatggcgcg cgccatgttc cggttctgcg ccaccgcgcg
     2941 aaccttgaga cccagcgagg tcttctgcag caccagctgc aggatcacga acacgacgat
     3001 catgaacagg atcacgtaca ggcggttgta ggtgatcgac agcgcctcgt tgatctgcat
     3061 ggagccggcc atccagcctg gcgtttccac gggacgattg ttggcgctga acacgtcgcg
     3121 caccacctgc tggagtatca ggctgacgcc gaacgtggct aacaaggttt cgagaggccg
     3181 gccatagagg aaacggacca cgccgcgctc gatcagcatg ccgacgcagg ccgacaccag
     3241 gaatgcgagt ggaatggcga cgaagatcga catcccgatg tgattgggca tcgccagctg
     3301 caccacatac gtggtgtagg cgccgagcat catcagctcg ccgtgggcca tgttgatgac
     3361 gcccatgacg ccgaaggtga tcgcgagccc gatggcggcg agcaccagca ccgatccgag
     3421 actcaggccg aagaagagag tctctatcgt cgaatacacg cggcgcgagg aatcgatgga
     3481 cgcgatgact tcgcgtgccg tggtgcgcac ccgttcgtct ggctccggga aggcgccatc
     3541 gacggcggtc accatggtcg tgagccggtt gtagacgtcg ggactcagcg aatccttcag
     3601 cgcctgcacc gctgagaggc gcgtatccgc ttcggtgctg tcgagatcgg ccagcgcgag
     3661 gccttcgcgc atggccttgc gcacgtcgtc gtcggattcc ttctcgacac tggcgcgcag
     3721 catcccaacg ctctcttcgt cgatcgaacg caacatctcg cgcaccgccg ccaggcgcac
     3781 accggcatcc ggatcggaca acgcgaaact ggcaacggcg ctgcgcaatg tcttgcgcag
     3841 gctgttgttg gtcgtgatgc gtgaaaactc ttcggggttg ccgctgccac tcgcggcgag
     3901 cgtcaaagga tcggtgagcg cgagttgggt ctcgccgcca tccgtgatga aaactttggt
     3961 gtctgaagtg cggacataca gcttgccgtc gagcaacgcc gccagcacca cccggccgtt
     4021 cgggtgtttg agttcggtca gcgtcgacac cgcggccgac ttctcgggaa aactttcggc
     4081 ggcgagcccg gcgacagccg aggcaaacgc atcgccaggt gcgggcgcgg tagtttgggc
     4141 ccgcacggcc gcggcgaaca atacggcgat cagcgtgaag accgcgacgt agccgaatcg
     4201 ccgcagaaac aaaagagaag gtcccgggat tgctcccggg acctcaaatg acttttcaac
     4261 cagctttttc agaatttttg ttctttgatt gcttacttgt aattctggcc cgagcactgc
     4321 ttggtcttgg tgttgtagtt gccgcacttc aacgtg
//
LOCUS       DATDEU010000239         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1937287, whole genome shotgun sequence.
ACCESSION   DATDEU010000239 DATDEU010000000
VERSION     DATDEU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937287"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..838
                     /locus_tag="VIV63_05920"
     CDS             188..838
                     /locus_tag="VIV63_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6124167.1"
                     /translation="MYKLHAFAQSGNTYKVAVLLQALRQPWTAIHLPFEEFAKGVQRS
                     DAWRQEVNPMGEIPVLEVGDKKLTQSAAIMLFLSNKHGAYGGQSEDERQEVLRWMFFD
                     NHKFTSYLATLRFLKSFGASAPDPAVMKFLAGRVDGAFGIVEKHLSNREYIVGSQPTI
                     ADMSLCGYLYFPAGESGYDIARSYPAMAEWLERLKSLPGWKPPYDMRPGQQIPPRW"
     gene            1091..>2216
                     /locus_tag="VIV63_05925"
     CDS             1091..>2216
                     /locus_tag="VIV63_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124168.1"
                     /translation="MLRSFKRFAGAWLVAVMLGGIAPQAFAQATYYTNSDNENNAGTH
                     VADNDMDVSLGNAAGIHPIEFNINVATLPQSSVVLTMRALDVDEEDGEVDEVYLNGHY
                     LGHLTGANNVWSSTAFNVNPAWLVAGQNLVRINVDVSGDPTAWVTTIDWAQLLTDGGG
                     ATDGDTQSVQITGTSVNAGNVTINTSTSVHSITGGNYRLQISLITPSGDAVTVLTQDF
                     AVAAGADVTRTANPTYPLNSVSGTYTIQAQLFWLDPSQGNFPVQQDIAIAQFTHTFGS
                     GASNFSNDSDGDGLLDSEEATLGTDANDADTDGDGTSDGAEVGPNLSSPVDTDGDGVI
                     NALESSIVDTDGDGVMNQIDPANSNPCIPNASFGSCPAGDL"
BASE COUNT          435 a          716 c          672 g          393 t
ORIGIN      
        1 catcgcggtg ctgctcgcca tgaactacgt gatcctgcca ctgtcggcca tcggtcgcgg
       61 catttatccg ctggcgcaga tgcacatcca cgcgttctgg cacatcgtgc tggtgggcct
      121 gccgacggcg ttcttcgtct cgcgcgcatt gaaggctccg acgcgctgac gatacactcg
      181 cggcacgatg tacaaacttc atgccttcgc ccaatccggc aatacctaca aggtcgcggt
      241 tctccttcag gcactccggc aaccgtggac ggctatccac ctgccgttcg aggaattcgc
      301 gaagggcgtt cagcgctcgg acgcctggcg ccaggaagtc aacccgatgg gcgaaatccc
      361 cgtgctcgag gttggcgaca agaagctcac gcaatccgcg gcgatcatgc tgttcctgtc
      421 gaacaaacac ggtgcgtacg gcggccaatc ggaggacgaa cgccaggaag tcctgcgctg
      481 gatgttcttc gacaaccaca agttcacgag ctatctcgcg acgctgcggt tcctgaagtc
      541 gttcggagcg tcggcgccgg atccggccgt catgaagttt ctggccggcc gcgtggacgg
      601 cgcgttcggc atcgtcgaaa aacatctctc gaaccgcgaa tacatcgtgg gttcgcaacc
      661 gacgatcgcc gacatgtcgc tgtgcggtta tctgtacttt ccggccgggg agagcggcta
      721 cgacatcgcc cggagttatc cggcgatggc ggaatggctc gagcggctca agtcgctgcc
      781 gggctggaaa ccgccgtacg acatgcggcc agggcaacag atcccaccgc gctggtagcg
      841 cgcatccgta gctaatagat aagagtgggg cccgcacagg gccccgcacg ccggccgacg
      901 ttatgtccgc ccgcttaaga ccaatcgcgg tctgctgtcc caccgttatt tgaatctcct
      961 ctccttctct ctcctaaggc agcccgccac aacgccgata cgaggcctgc acacgaggaa
     1021 tccgcaaaag gttgcggcgt aagcgccaag acattggcgt cgggttggta gtacgggagt
     1081 tgtcataagc atgcttcgtt cattcaaacg gttcgcgggc gcctggttgg ttgcggtcat
     1141 gctgggcggg attgccccgc aggcatttgc gcaggcgact tactacacga actcggacaa
     1201 cgagaacaac gcgggcacgc atgtcgccga caacgatatg gacgtttcgc tgggcaacgc
     1261 cgccggcatc cacccgatcg aattcaacat caacgtcgcg acactgccgc agagctccgt
     1321 ggtgctcacc atgcgcgcgc tcgacgtgga cgaagaggac ggcgaggtcg acgaggtcta
     1381 cctcaacggc cactacctgg gtcatctcac gggcgccaac aacgtctgga gctccaccgc
     1441 tttcaatgtg aatcccgcct ggctggtcgc cgggcagaat ctcgtgcgca tcaatgtcga
     1501 cgtgagcggt gatcccacgg cgtgggtcac gacgatcgac tgggcgcagt tgctgaccga
     1561 cggcggcggc gccaccgatg gcgatacgca gagcgtgcag atcaccggta cgtcggtgaa
     1621 cgcgggcaac gtcaccatca acacttcgac gtcggtgcac tcgatcaccg gcggcaacta
     1681 tcggctgcag atctccctca tcacgccgag cggcgatgcg gtgacggtgc tcacgcagga
     1741 cttcgcggtc gccgcaggcg cggacgtgac gcgcacggcg aaccccactt atccgctgaa
     1801 cagcgtctcg ggtacctaca ctatccaggc gcagctcttc tggctcgatc cctcgcaggg
     1861 caatttcccc gtgcagcagg acatcgcgat cgcgcagttc acgcacacat tcggatcggg
     1921 cgcgagcaac ttcagcaacg acagcgacgg cgatgggctg ctcgacagcg aggaagcgac
     1981 gctcggcacc gacgccaacg atgcggatac cgacggtgac ggcaccagcg acggcgctga
     2041 agtcggcccg aacttgagct cgccggtcga caccgacggt gatggcgtca tcaacgcact
     2101 cgaatcgtcg atcgtcgata ccgacggcga cggcgtcatg aatcagatcg atcctgccaa
     2161 ttccaatccg tgcatcccga acgcgagctt cgggtcttgc ccggcaggcg acctcg
//
LOCUS       DATDEU010000240         2848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1937668, whole genome shotgun sequence.
ACCESSION   DATDEU010000240 DATDEU010000000
VERSION     DATDEU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937668"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..472)
                     /locus_tag="VIV63_05930"
     CDS             complement(35..472)
                     /locus_tag="VIV63_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6124169.1"
                     /translation="MPRRLRMAMTVAGGAWLAASWVAHGETPAATVDSRPPGQRLYAS
                     HCLSCHQADGGGVPNMQPAIAGGTWVKGDAKALALFVMSGGFDSASRKDSAEGNVMPP
                     FSQLADADLAEILTYIRQKFGEGASAVSAAEVAEARTSLVAPP"
     gene            complement(472..1719)
                     /locus_tag="VIV63_05935"
     CDS             complement(472..1719)
                     /locus_tag="VIV63_05935"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY6124170.1"
                     /translation="MRNAWLARVVSAAGIGYTMAAAGAAFAADNDGLKLAEGFSAIVV
                     HEGQGTARHLDVAANGDIYIAGRNGLSAMRDTNRDGKADIVSAFGDVKGTEVRVFKNW
                     LYVSDDVGVYRYPLKKGQLAPTGNRETVVSEFPKERQHSDKTFALDAKGTLYINVGAP
                     SNSCQEKDRQEGSLGLNPCPILEKYGGVWVFDGTKLNQTPANGRRFATGMRNAVAIEW
                     NAAQNALFSVIHGRDSLDTLFPALYNAEDNATRQAEEFHQIVDGGNYGWPNTFFDNKL
                     GKRVLAPEYGGDGTKEPEAGKYPAPLVAYPAHWAPNDLLFYSGKKFPAKYRNGAFIAF
                     HGSWNRAPEPQAGYKVVFQPMKDGKPSGDYEVFADGFAGEMKDNSPRNAQYRPVGLAM
                     TPDGSLLISDSQKGRIWRISYGK"
     gene            2050..2661
                     /locus_tag="VIV63_05940"
     CDS             2050..2661
                     /locus_tag="VIV63_05940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6124171.1"
                     /translation="MPLVDPVIERVLADFERRAEDEQRRTTTPGTNLDELLLSVGREA
                     GTLLYLLATGAQSRRILELGTSYGYSTVWLAAAARTTGGKVLSLELKDYKIEHARQAL
                     TRAGLSTRVEFHAGDCLDSLRTLPGPFDFVLLDVWKDLYLPCFELVHPKLASGGIIAA
                     DNMLLPESARPHADAYRARVRAAGDMDSVLLNVGNGIEISRKR"
     gene            complement(2665..>2848)
                     /locus_tag="VIV63_05945"
     CDS             complement(2665..>2848)
                     /locus_tag="VIV63_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124172.1"
                     /translation="LQIGANIAGIIFVIASMHVLYLNTHVLPVALRPPMWRRVALVGM
                     ALFYGYFVTLVARTFL"
BASE COUNT          499 a          954 c          888 g          507 t
ORIGIN      
        1 ctaggacgga attggggact gacccccaac ttccttatgg gggcgccacc aaactagtac
       61 gcgcttcggc gacttccgcg gcgctcaccg cggaagcacc ctcgccgaac ttctgccgga
      121 tgtaggtgag gatctcggcg aggtcggcat cggcaagctg gctgaacggc ggcatcacgt
      181 tcccttccgc gctgtccttg cgcgaggccg aatcgaagcc gccgctcatc acgaacagcg
      241 cgagagcctt cgcatcaccc ttgacccaag tcccgccggc gatggcgggc tgcatgttgg
      301 gcacgccgcc gccgtccgcc tgatggcagc tcagacaatg actggcatag agacgctggc
      361 ccggcggccg tgaatcgacc gtcgccgcgg gcgtttcgcc gtgggcaacc catgacgccg
      421 cgagccacgc gccacccgcc acggtcatcg ccatgcgcaa tcgacgcggc atcatttccc
      481 gtacgagatg cgccagatgc ggcccttctg cgagtcggaa atcagcagcg aaccatccgg
      541 cgtcatcgcg aggcccaccg gccggtactg cgcattgcgc gggctgttgt ccttcatctc
      601 gccggcgaat ccgtcggcga acacttcgta gtcaccgctc ggcttgccgt ctttcatggg
      661 ctgaaacacg accttgtaac cggcctgcgg ctcgggagcg cgattccagc tcccgtgaaa
      721 ggcgatgaag gcgccgttgc gatacttcgc cgggaatttc ttgcccgaat agaacagcag
      781 gtcgttaggc gcccaatgcg cgggatacgc caccagcggc gccgggtact tgccggcctc
      841 gggttccttc gtaccgtcgc cgccgtattc gggtgcgagc acgcgcttgc cgagcttgtt
      901 gtcgaagaac gtgttcggcc agccgtagtt gccgccgtcg acgatctgat gaaactcttc
      961 ggcctggcgc gtcgcattgt cttcggcgtt gtagagcgcg gggaacagcg tgtcgagcga
     1021 gtcgcgaccg tggatcacgg agaacagcgc gttctgcgcg gcattccact cgatggccac
     1081 ggcattacgc atgccggtgg cgaagcgccg gccgttcgcc ggtgtctggt tgagcttcgt
     1141 gccgtcgaac acccatacgc cgccgtattt ctcgaggatg ggacaggggt tcaacccgag
     1201 cgaaccttcc tgccgatcct tttcctggca ggagttcgaa ggcgcaccca cgtttatgta
     1261 gagcgtgcct ttcgcatcca gcgcgaaagt cttgtcggaa tgctggcgct ccttcggaaa
     1321 ttcgctgacc acggtttcgc gattgccggt gggcgcgagc tggcccttct tgagcggata
     1381 gcggtacacg ccgacgtcgt cggagacgta cagccagttc ttgaacacgc gaacttcggt
     1441 gcccttcacg tcgccgaagg ccgaaacgat gtcggccttg ccgtcgcgat tcgtatcgcg
     1501 catcgcggag aggccgttgc ggccggcgat gtagatgtcg ccgttggcgg ccacgtcgag
     1561 gtggcgggcg gtgccctggc cttcgtgcac cacgatcgcg gaaaaccctt ccgccagttt
     1621 caaaccgtcg ttgtcggcgg cgaacgccgc gccagcggcg gccatcgtgt agccgatacc
     1681 tgcggcacta actactctag cgagccacgc attgcgcatg tttccattcc ttgcgatctg
     1741 gttgattcga cgaaggtcgc aacggaaaac gcccgcgacc cctcgtccgg acggcgatag
     1801 tgcgggcgaa tttcccgcaa cgcaacggcg gcggcgccgg gccggcgcac ccccgggcta
     1861 gcacgcgttt ccggcctgcg ccagtccaaa agtgtcatga cgtcctacaa accccggggg
     1921 attcgtgcac gaatttcgcg ctccatgact ggctagcaaa ctttttcgcg gaggctcgcc
     1981 aacccgcgga caaacatgca ctatgcggcc cgtcgaggcc ccaaggcccc gcgaaccaga
     2041 agactccgca tgcctctagt cgatcctgtc atcgaacgcg tgctcgcaga tttcgagcgc
     2101 cgcgcggaag acgaacaacg ccgcacgacg acccccggca ccaatctcga cgaactcctg
     2161 ctgtcggtcg gccgcgaggc cggcaccctg ctctatctac tggcgacggg agcgcaatcg
     2221 cggcgcatcc tcgagctcgg cacctcgtac ggctactcca cggtgtggct ggccgcggcg
     2281 gcacgcacca ccggcggcaa ggttctgtcg ctcgaactca aggactacaa gatcgaacat
     2341 gcgcggcagg cgctgacgcg cgccggcctt tccacgcgcg tggagttcca cgcgggtgac
     2401 tgcctcgatt ctttgcgcac gttgcccggc ccgttcgact tcgtgctgct cgacgtctgg
     2461 aaagacctgt atctcccctg cttcgagctc gtgcacccca agcttgcgag cggcggcatc
     2521 atcgccgcgg acaacatgtt gttgcccgag agcgcgcgcc cgcacgccga cgcctatcgc
     2581 gcgcgcgtgc gcgccgcggg agacatggat tcggtgctgc tcaacgtggg caacggcatc
     2641 gagatcagca ggaagcgctg acgctcacaa gaacgtgcgc gccaccagcg tgacgaagta
     2701 gccgtagaac agcgccatgc cgacgagcgc cacccggcgc cacatcggcg gccgcagcgc
     2761 gaccggcagt acatgtgtgt tcaggtacag aacatgcatc gaagcgatca cgaagatgat
     2821 cccggcgatg ttcgcgccga tctgcagt
//
LOCUS       DATDEU010000241         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1063945, whole genome shotgun sequence.
ACCESSION   DATDEU010000241 DATDEU010000000
VERSION     DATDEU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063945"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1980
                     /gene="htpG"
                     /locus_tag="VIV63_05950"
     CDS             49..1980
                     /gene="htpG"
                     /locus_tag="VIV63_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY6124173.1"
                     /translation="MSGNPAAETTPPEGNGPSGTPTGFETRGFKAEVRELLKLMIHSL
                     YSHREIFLRELISNASDANDKLRFEALSKPDLMTGDTELAVWIEADAEKKTLTIRDNG
                     IGMSREEAVSHLGTIARSGTAEFFKKLSGDQQKDSQLIGQFGVGFYSAFIVAERVEVL
                     TRRAGLPASDGVRWESAADGDFTVETLELAERGTTIVLHLKDDAKEFLDAWRLRSLVR
                     RYSDHIAFPVRMPKEGEATVEYEAVNRGTALWTRPKGDIKDEEYVEFYRSLSHDSGEP
                     LTWSHNRVEGKRDYTSLVFIPAAAPFDLWQRDGARGLKLYVRRVFIMDDAEQFLPLYL
                     RFVKGVLDSADLPLNVSRELLQSDSEVEAMRAALTKRVLDLLAKLAKDDAAKYQGFWN
                     EFGAVLKEGLAEDYGNRDKLLPLLRFASTNDAGDTEAVSLADYVARMQTGQDKVYYVV
                     GENFAAARSHPAIEALRARNVEVLLLGRRIDAWVMDHLQEFEGKQFRDASRGDLDLGA
                     LASTEDKEKAEAQIKESEPLLKRVKDALGDRVSEVRVSTRLKDSAAVLVAAEHDISAP
                     LRRALEAAGQKAPAGKPVLELNVDHHLVKYLGQRDDTADFGELALVIFEQAMLAEGAT
                     LADPGAFVQRLNRMWARLQ"
     gene            2026..>2489
                     /locus_tag="VIV63_05955"
     CDS             2026..>2489
                     /locus_tag="VIV63_05955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY6124174.1"
                     /translation="MHRKSLANSFTLMLTALAIVACSRNESPESAEASAPTAEAAPAP
                     ATEPAVTPSTGTAPMPLQKTELAPGKGADIKSGQTALVHYTGWLFDAAAPDNKGKQFD
                     SSVGGEPFEFPLGAGAVISGWDQGVVGMQVGGKRRLVIPPEMGYGNRGAGGV"
BASE COUNT          472 a          818 c          817 g          382 t
ORIGIN      
        1 cggcgcgccc ctatgctcgg cggtccactg ccgcgcgaga gaaggtgcat gtccgggaac
       61 cctgccgctg aaaccacccc gccggagggc aatggcccat ccggaacccc cactggcttc
      121 gagacacgtg gcttcaaggc cgaggtgcgt gagctgctga agctcatgat ccattcgctc
      181 tacagccacc gggaaatctt cctgcgcgag ctgatctcga atgcctccga tgccaacgac
      241 aagctgcgct tcgaggccct gagcaaaccc gatctcatga ccggcgacac cgaactcgcc
      301 gtctggatcg aggcggacgc cgagaagaag acgctgacca tccgcgacaa cggcatcggc
      361 atgagccggg aggaagccgt gtcgcacctg ggcacgatcg cccggtccgg gaccgcggaa
      421 ttcttcaaga agctctcggg cgaccagcag aaggattcgc agctcatcgg ccagttcggc
      481 gtgggtttct actcggcgtt catcgtcgcc gagcgcgtcg aggtactgac gcggcgcgcg
      541 ggtctgccgg ccagcgacgg cgtgcgctgg gaatcggccg cggacggcga cttcacggtg
      601 gagacgctcg aactcgcgga acgcggcacg accatagttc tgcacctgaa agacgacgcg
      661 aaggagttcc tcgacgcctg gcgcctgcgc tcgctggtgc gccgctactc ggaccacatc
      721 gcgttcccgg tgcgcatgcc gaaagaaggc gaggccacgg tcgagtacga ggccgtcaac
      781 cgcggtacgg cgctctggac gcgtcccaag ggcgatatca aggacgagga gtacgtcgag
      841 ttctaccgca gcctgtccca tgactcgggc gagcctctga cctggtcgca caaccgcgtc
      901 gaaggcaaac gtgactacac cagtctcgtg ttcatcccgg cggcggcgcc gttcgacctc
      961 tggcagcgcg atggcgcgcg cgggctcaag ctgtacgtgc gccgcgtgtt catcatggac
     1021 gacgcggagc agttcctgcc gttgtacctg cgcttcgtca aaggcgtgct cgattccgcc
     1081 gacctgccgc tgaacgtgtc gcgcgaactc ctgcagtcgg acagcgaagt ggaagccatg
     1141 cgtgcggcac tgaccaaacg cgtgctcgac ctgctggcca aactcgccaa ggacgacgca
     1201 gcgaaatatc agggcttctg gaacgagttc ggtgcggtcc tcaaggaggg actggccgag
     1261 gattacggca atcgcgacaa gctcctgccg ctgctgcgct tcgcatcgac gaacgacgcg
     1321 ggcgacaccg aagcggtctc gctggccgac tacgtggcgc gcatgcagac gggccaggac
     1381 aaggtctact acgtggtggg cgagaatttc gcggcggcgc gcagccaccc ggccatcgaa
     1441 gcgctccggg cgcgcaacgt cgaggtgctg ttgttaggcc ggcgcatcga cgcctgggtc
     1501 atggaccacc tgcaggagtt cgaaggcaag cagttccggg acgcctcgcg aggtgatctc
     1561 gatctcggcg cgctcgcgag caccgaggac aaggaaaagg ccgaggcaca gatcaaggag
     1621 agcgaaccac tgctcaagcg cgtcaaggat gcgctgggcg accgggtgtc cgaggttcgc
     1681 gtgagcacgc ggctcaagga ttccgcggcg gtgctcgtgg ccgccgaaca cgacatctcc
     1741 gcgccgctgc ggcgagcact ggaggccgcc ggccagaagg cgccggccgg caagccggtg
     1801 ctcgagctaa atgtcgacca ccacctcgtg aaatatctcg ggcaacgtga cgacaccgcg
     1861 gacttcggtg agctggcgct ggtgatattc gagcaggcaa tgctcgcgga aggtgcgacg
     1921 cttgccgatc cgggcgcgtt cgtccagagg ctcaatcgca tgtgggcgcg cttgcagtag
     1981 agattgtttg caataatgcc gctcccgcaa ttccggagtc tcgcgatgca ccgcaagtca
     2041 ctggcgaatt cattcacgct gatgttgacc gcgctcgcca tcgtggcctg cagcaggaac
     2101 gagtcaccgg aaagcgcgga ggcatccgcg cccaccgccg aagccgcgcc ggcgcccgcc
     2161 acggaacccg ccgtaacacc ttccacagga accgcaccca tgcccctgca gaaaactgaa
     2221 ctcgctcccg gcaaaggcgc cgacatcaaa tccggccaga ccgcgctggt gcactacacc
     2281 ggctggctgt tcgacgccgc cgcgcccgac aacaagggca aacaattcga cagctccgtg
     2341 ggcggcgagc ccttcgagtt tcctctcggt gccggcgcgg tcatctccgg ttgggaccag
     2401 ggcgtggtcg gcatgcaggt cggtggcaaa cgccgcctcg tcatcccgcc tgaaatgggc
     2461 tacggcaacc gcggcgccgg cggcgtgat
//
LOCUS       DATDEU010000242         2888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_333138, whole genome shotgun sequence.
ACCESSION   DATDEU010000242 DATDEU010000000
VERSION     DATDEU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333138"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..937)
                     /locus_tag="VIV63_05960"
     CDS             complement(248..937)
                     /locus_tag="VIV63_05960"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEY6124175.1"
                     /translation="MAEITNSASQRIRSRLQAKKQRFHANDNISAFIEPGELEELQKE
                     VAAKMAGVLESLVIDVEHDHNTRETARRVAKMYVNEVFRGRFVPMPNVTEFPNASHLN
                     ELMIMGPVTVRSACSHHLCPIMGRLWIGVMPNQQSNLIGLSKYSRLVEWIMTRPQIQE
                     EAVLQLADILVERVNPDGLAVVMEADHFCMHWRGVKDNASKMTNSVMRGVFLKDGALR
                     REFLSLINLKT"
     gene            1017..>2888
                     /locus_tag="VIV63_05965"
     CDS             1017..>2888
                     /locus_tag="VIV63_05965"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124176.1"
                     /translation="MNAKITPIHGPLKFLAGGGEMGELIRNFDWANSVLGPPERWPSG
                     LRTSLRTMLTTQHPVFIFWGAELTCFYNDAYSRSLGPEKHPSILGMPAQQAWPEIWHI
                     IGSQIDQVMRGDTATWHENQLVPIIRHGELQDVYWTYSYGPIDEPAAPHGVGGVLVIC
                     TETTQQILTERRITAERERFAQLFEQAPTFMCVLRGPDHVFELANPGYMRLIGHRSII
                     GKTVAEALPEVIEQGYIKLLDQVYESGEAFLAIAAPYAYQAQPDAPATQVFLDFVYQP
                     IKDADGSVSGIFVEGADVTPRAMADSALRDADRRKDEFLAMLAHELRNPLAPISTAAE
                     VLSRRIVNDETARRAADVVRRQATQLTRIVDDLLDVSRISMGHVELKRETLLISDVID
                     LAVETVTPLWRAKQHQITTHASLEPVYVRGDRARLVQSLGNVMTNAAKYTDAGGEIGI
                     DVSATADSVTVEISDTGAGISPEFMPRLFELFAQADRTLDRSQGGLGIGLSVVKKLVE
                     MHGGDISARSDGLGRGSVFQIVLPRVEAPLPQSAEAGAAAVKARRILIVDDNADAASS
                     LSELLKMDGHQTQPAFSAEEALSLAQSFDPDIVLLDIGLPRMDGYEVARRLRHSIVRK
                     "
BASE COUNT          532 a          950 c          887 g          519 t
ORIGIN      
        1 gacctcgatc atcggcccct tgagataact catcagggcc tgcctcaccg gctcggccac
       61 atcggcgctc gcgccgttgg gctggccgag ctgcgcccgg ggcatcgcca ccccgaccgt
      121 gagtttggtg tcggcaaaag cggcgttcac gagcccgagg cccagaaaag ccgacgtgag
      181 acagattgtt actgcgcgca tacatcgtcc tcctgatgta tggagcgtaa aaactatcgg
      241 cgagggatca cgtcttcagg ttgatgagcg acaggaactc gcgccgcaac gcgccatcct
      301 tgaggaacac gccgcgcatg acgctgttgg tcatcttcga ggcgttgtcc tttacgccgc
      361 gccagtgcat gcagaaatgg tccgcctcca tcaccacggc aaggccgtcg gggttcacgc
      421 gctccacgag gatgtcggcg agctgcagca cggcctcttc ctggatctgc ggccgcgtca
      481 tgatccactc cacgagccgc gaatacttgg agagcccgat gaggttcgac tgctggttag
      541 gcatgacgcc tatccacagg cggcccatga tgggacacag gtgatggctg caggcgctgc
      601 gcaccgtcac cgggcccatg atcatgagtt cgttcaggtg tgaggcattc ggaaattccg
      661 tgacgttcgg catgggcacg aaccgcccgc ggaacacctc gttcacgtac atcttggcga
      721 cgcgccgcgc ggtttcgcgc gtgttgtggt cgtgctcgac gtcgatgacg aggctctcga
      781 gcaccccggc catctttgcc gccacctcct tctgcagctc ctcgagctcg cccggctcga
      841 tgaacgcgga gatgttgtcg ttggcatgaa atcgctgctt tttcgcctgc aggcggctgc
      901 ggatgcgctg cgacgcggaa ttggtaattt ctgccatttc atccaccgac taagggctgc
      961 gggaaggccg cagagccttg catgttcgcc ctcgtctgac aaacttggcc acctcgatga
     1021 acgccaaaat cactcccatt cacggcccgc tcaagttcct ggctggcggc ggcgagatgg
     1081 gcgagctcat tcgcaacttc gactgggcca attcggtgct ggggccaccc gagcgctggc
     1141 cgtcgggcct gcgcacgtcg ctgcgcacga tgctcacgac ccagcatccg gtgttcatct
     1201 tctggggcgc cgagctcacc tgcttttata acgacgccta cagccgatcg ctgggcccgg
     1261 aaaaacatcc gtcgatcctc ggcatgcccg cccagcaggc ctggcccgag atctggcaca
     1321 tcatcggttc gcagatcgac caggtgatgc gcggcgacac cgccacctgg cacgaaaacc
     1381 aactcgtccc catcatccgc cacggcgagt tgcaggacgt ctactggacc tacagttacg
     1441 gccccatcga cgagccggcc gcgccacatg gtgtcggcgg cgtcctggtg atctgcaccg
     1501 agaccaccca gcagatattg accgaaaggc gcatcaccgc cgaacgcgaa cgtttcgcgc
     1561 agctcttcga gcaggctccc acgttcatgt gtgtgctgcg cggacccgac catgtgttcg
     1621 agctggcgaa tccgggctac atgcggttga taggccatcg gtcgatcatc ggcaaaacgg
     1681 tagccgaggc gctgcccgag gtcatcgaac agggctacat caaactgctc gaccaggttt
     1741 acgaatcggg agaagcgttc ctggcgatcg ccgctcctta tgcgtaccag gcgcagcctg
     1801 acgctccggc aacccaggtt ttcctcgact tcgtctatca accgatcaag gacgccgacg
     1861 gcagcgtctc cggcatcttc gtcgaaggcg ccgacgtcac cccgcgcgcc atggcggact
     1921 ccgcgctgcg cgacgccgac cgacgcaagg acgaattcct ggcgatgctc gcgcacgaac
     1981 tgcgcaatcc gctggcgccg atcagcacgg cggccgaagt cctctcgcgg cggatcgtca
     2041 acgacgagac ggcgcggcgc gcggccgatg tcgtgcggcg gcaggccacg cagctcacgc
     2101 gtatcgtcga cgacctgctc gacgtatcgc gcatctccat gggtcacgtc gaactcaaac
     2161 gcgaaacgct gctgatatcc gacgtcatcg atctcgcggt cgaaaccgtg acgccgctgt
     2221 ggcgcgcgaa gcagcatcag atcacgacgc acgccagcct cgaacccgtg tatgtacgcg
     2281 gtgatcgcgc gaggctcgtg cagagtctcg gcaacgtgat gacgaacgcc gcgaaataca
     2341 ccgacgcggg cggtgagatc ggcatcgacg tctccgcgac cgcggactcg gtgaccgtgg
     2401 aaatcagcga caccggcgcc gggatctcgc cggaattcat gccgcgactc ttcgagcttt
     2461 tcgcacaagc ggatcgcacg ctcgaccgct cgcaaggcgg cctggggatc ggtttgtcgg
     2521 tggtcaagaa actcgtggag atgcacggcg gagacatttc ggcgcgcagc gatggcctgg
     2581 gacgcggctc cgtgttccag atcgtgcttc cgcgcgtcga agcgcccttg ccgcaatcag
     2641 ccgaagcggg cgccgccgcc gtgaaggcgc gccgcatcct gatcgtcgat gacaatgccg
     2701 acgccgcgag ttccctgagc gagctgctga aaatggacgg acaccagaca cagccggcgt
     2761 tctccgcgga agaagcgctg tcgctggcac agtccttcga tccggacatc gtgctgctcg
     2821 atatcggttt gccgcgcatg gatggttacg aagtcgcgcg gcggctgcgt cattcgatcg
     2881 ttcgcaag
//
LOCUS       DATDEU010000243         3865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2084858, whole genome shotgun sequence.
ACCESSION   DATDEU010000243 DATDEU010000000
VERSION     DATDEU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084858"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VIV63_05970"
     CDS             <1..236
                     /locus_tag="VIV63_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124177.1"
                     /translation="PLGNEGIELVDGVHAMISAEGADMAAKLNAAARDPDYVARLGAA
                     AAAHIRTQFRQEAVATRLIDLLRSPSRPGELAG"
     gene            332..1072
                     /locus_tag="VIV63_05975"
     CDS             332..1072
                     /locus_tag="VIV63_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6124178.1"
                     /translation="MSTAWGYQAYMAMRWGTQRRDAPRVNPVNTVLKTRAEVSAAVAE
                     ATRLKLPQFSDPPKTWDTLGALHEIVSRTDTNAKILDAGAEMYSRILPWLYLYGYRNL
                     TGNNLIFDKSKTVKRGTIAYEYGDITATRFDDKHFDAITCLSVVEHGVDLQKYFAEMS
                     RILKPGGVLVTSTDYFEQPTDTRGLSAYGTPIRVFTRADIEKAVEGAKKVGLRLTQPI
                     DYSSQERVVTWKQFGLSYSFIIFSMIRQ"
     gene            complement(1080..2636)
                     /locus_tag="VIV63_05980"
     CDS             complement(1080..2636)
                     /locus_tag="VIV63_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6124179.1"
                     /translation="MLLHELLDRSASVTPEAVALSYRASSSTYAEVRAASLRLAAGLA
                     GLGIERGDRIAVHLANRPEAVELALAASRIGAIFMPLNPGLRARQLAHVLKDSGARML
                     VTSGAIAPQLIGTTPLPALEFLVVSDALTDVPDAGVRAMRLQDLHERGSFSDTASHGA
                     SPYDLAALFYTSGSTGLPKGVMLSHANLTSGAETVSRYLGNEPTDRILVALPWSFDYG
                     FNQIATALAVGACAVLTNYASAAALINEVYDERITALAGVPTMWMQLSAARWRATPPE
                     TLRYITNSGGALHAATIRALQALLPGTRIFCMYGLTEAFRSTYLDPAELPRRIGSIGK
                     AVPGQQVVVLDESGRECAPDEIGELVHRGSFVSRGYWNDDTLTQRRFRPFPGDRNGEL
                     AVWSGDLARRDADGFLWFVGRGDDQIKISGVRVSPTEIETVAAEVQGVEMCVAVGVAD
                     ELLGQRIVLFVTAASPSVDLTDRIREHCRRQLPNYAAISAVVFEPDLPLTATGKPDRA
                     VLAARAADSC"
     gene            2709..3722
                     /locus_tag="VIV63_05985"
     CDS             2709..3722
                     /locus_tag="VIV63_05985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6124180.1"
                     /translation="MHSLKQLIRRSIASALYYTGALYLYARVVARNRVVVLTYHRVLP
                     SDKAAASFSSSAIIVTPDTFAMHMETVARWFKPLNAGTFAGHSGGRACLVTFDDGWYD
                     NHVYALPVLERTRVPMLLFVATNYIGTSDCFWQETLAAHLYAARGPRGNGEAKLVFDE
                     LGARELGHLDGDEARRAALAAVARVKARKLNPAHLLQRLREAVPATAKSASPEDRFLT
                     HAELKKLMASPCITLGSHSMTHSRLPLESDAVIEAELEGSRDILIEWTGKRPATLAYP
                     NGDHDERVVAAARAAGYRVAFTTETGPHVPGSDLLRVRRFNMHEAAVNTKPLFLSRLL
                     GVL"
BASE COUNT          703 a         1303 c         1224 g          635 t
ORIGIN      
        1 ccccgctcgg caacgagggc atcgaactcg tcgatggtgt acacgccatg atcagcgccg
       61 aaggcgccga tatggccgcc aagctcaatg ccgcggcgcg cgacccggat tacgtcgcaa
      121 ggcttggcgc cgccgccgcc gcccatattc gaacccaatt ccgccaggag gccgtagcca
      181 cgcgcctgat cgatttattg cgctccccgt cacgccccgg agaactggcg ggttgacggc
      241 cgcatgggcc ctggcccgga cagttaagat ttcgccgctt tttctacttc aaacgagccc
      301 aaacatgtcg attcccggcc gagatgcatt gatgtccacc gcctggggct atcaggccta
      361 catggccatg cgctggggca cccagcgtcg cgatgcgccc cgcgtgaacc cggtcaacac
      421 cgtgctcaaa acccgcgccg aagtcagcgc tgccgtggcc gaagccacgc gcctgaagct
      481 gccgcagttc agcgatccgc cgaagacctg ggacaccctg ggcgcgctgc atgaaatcgt
      541 cagccgcacc gacaccaatg cgaagattct cgatgccggc gcggagatgt actcgcgcat
      601 cctgccgtgg ctctatctat acggttaccg gaacctcacc ggcaacaacc tgattttcga
      661 caagagcaag accgtcaagc gcggcacgat cgcctatgaa tacggcgaca tcacggccac
      721 gcgattcgac gacaaacact tcgacgccat cacctgtctg tcggtggtcg agcatggcgt
      781 ggatctgcag aaatacttcg ccgagatgtc gcgcatcctg aagccgggtg gcgtgctggt
      841 cacgtccacg gactacttcg agcagcccac cgacacgcgc ggactgtccg cgtatggcac
      901 gccaatccgc gtgttcacgc gcgccgatat cgagaaggca gtcgaaggtg cgaagaaggt
      961 cggactgcgg ctcacgcagc ccatcgacta cagctcgcag gagcgcgtcg tcacctggaa
     1021 gcagttcggg ctgtcgtact cgttcatcat cttttcgatg attcgtcagt aaacctggtt
     1081 cagcaggaat cggcggcacg cgcggcaagc acggcccggt ccggcttgcc cgtggccgtc
     1141 aacggcaggt ctggctcgaa taccacggcc gagatcgccg cgtagttagg gagttggcgg
     1201 cggcaatgtt cgcgaatgcg gtcggtgaga tccacagagg gcgatgcggc cgtgacgaag
     1261 agcacgatgc gctgccccag cagttcgtcc gcgacgccca cggctacgca catttcgacg
     1321 ccctgcacct cggcggccac cgtttcgatt tccgtgggac tcacccgcac accggagatc
     1381 ttgatctggt catcgccgcg gcccacgaac cacaggaatc catcggcgtc gcgccgtgcg
     1441 agatcgccgg accacaccgc cagttcgccg ttccggtcgc ctgggaaggg ccggaagcgc
     1501 cgttgcgtga gggtgtcgtc attccagtag ccccgcgaga cgaacgagcc gcgatgcacc
     1561 agctcgccaa tttcgtccgg cgcacattcc cggcccgatt cgtcgagcac cacgacctgc
     1621 tggccgggca cggccttgcc gatcgagccg atgcggcgcg gcagttcggc cgggtcgaga
     1681 taggtggagc ggaacgcctc ggtcagaccg tacatgcaga agattcgcgt gccgggcaaa
     1741 agtgcctgca acgcgcggat ggtggccgca tgcaacgcgc cgccggaatt ggtgatgtaa
     1801 cgcagcgtct ccggcggcgt tgcgcgccag cgggcggcag agagctgcat ccacatcgtc
     1861 ggcacgccgg ccagcgccgt gatgcgttcg tcgtagacct cgttgatcaa cgccgcggcc
     1921 gatgcgtagt tagtcagcac cgcgcacgcg ccgacggcca atgccgtggc gatctggttg
     1981 aagccgtagt cgaaactcca cggcagcgcc acgaggatgc ggtcggtggg ttcgttgccc
     2041 aggtaccggg aaaccgtttc cgcgccggac gtgagattgg cgtgcgacag catgacgccc
     2101 ttcggcaggc cggtgctgcc cgacgtatag aagagtgccg cgaggtcgta agggctggcg
     2161 ccatgcgagg cagtgtcgct aaaggagcca cgctcatgca gatcctgcag ccgcatcgcg
     2221 cgaacaccgg catctggaac gtcagtcagc gcatccgaca cgaccaggaa ctcgagggcg
     2281 ggcaacggcg tggtgccgat gagctgcggg gcgatggcgc cgctcgtaac cagcatgcgt
     2341 gcgccgctgt ccttgagcac gtgcgcaagc tggcgcgcgc gcagtccggg attgagcggc
     2401 atgaaaatgg cgccgatgcg gctggccgcc agcgcgagct cgaccgcctc gggacggttg
     2461 gccaggtgca cggcaatccg gtccccgcgt tcgatgccga gcccggcgag acccgcggcc
     2521 aggcgcaacg aggccgcgcg tacctcggca taggtcgacg acgaagcacg atagctcagg
     2581 gccacggctt ccggcgtgac actcgcgctt cggtccaaca actcgtgcag caacatctgg
     2641 gcgtgatgtt aacctcacgc cgcgaaacgc gcgcgtgatg cggtgcgcgt taccgccccg
     2701 gtacctccat gcatagtttg aaacagctca ttcgccggtc gattgcctcc gcgctctact
     2761 acacgggcgc gttgtacctg tacgcgcgcg tcgtcgcccg caatcgcgta gtcgtactca
     2821 cctatcaccg tgtgctgccg tccgacaagg cggcggctag cttctcctcg tcggcgatca
     2881 tcgtcacgcc cgacactttc gccatgcaca tggaaaccgt ggcgcgctgg ttcaaacctc
     2941 tgaacgccgg cacgttcgcc gggcactcgg gcggccgcgc ctgtcttgtc acgttcgacg
     3001 acggctggta cgacaaccac gtgtacgccc tgcccgtgct ggaacgcacc agggtgccca
     3061 tgctgctgtt cgtggccact aactacatcg gcaccagcga ctgcttctgg caggagaccc
     3121 tggccgccca tctgtacgcc gcgcgcggcc cgcgcggcaa tggcgaggcg aaactggtgt
     3181 tcgatgaact gggcgcgcgc gaactgggcc atctggacgg cgacgaagcg cggcgcgcgg
     3241 cgctggcggc cgttgcacgc gtcaaggcgc gcaagctcaa ccccgcacac ctccttcagc
     3301 ggctgcgcga agccgtgcct gcgacggcca aatccgccag tcccgaagac cgctttctca
     3361 cacatgccga attgaagaag ctgatggcga gcccctgcat cacgctgggc tcgcactcca
     3421 tgacgcactc gcggttgccg ctcgagagcg acgccgtcat cgaagccgaa ctcgaaggtt
     3481 cgcgcgacat tctcatcgag tggaccggca agagacccgc gacactggcg tatccgaacg
     3541 gcgatcatga cgagcgcgtg gtcgccgccg cgcgcgccgc cggttatcgc gtggcattca
     3601 ccaccgaaac gggaccgcac gttcccggca gcgacctgct gcgggtacgg cgtttcaaca
     3661 tgcacgaggc cgcggtcaat acgaagccgt tgttcctcag ccgcttgtta ggtgtgctct
     3721 agcgcatgcg cgtactcgtc accgacagcg acaatcgttc ggcacttgcc accacccgca
     3781 gcctcggccg cgcgggttac gaagtgctgg tcatgggcga gcgcgaagcc agcatcgccg
     3841 ccgcctcgcg gtattgcagc cgttt
//
LOCUS       DATDEU010000244         2921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_481190, whole genome shotgun sequence.
ACCESSION   DATDEU010000244 DATDEU010000000
VERSION     DATDEU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481190"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VIV63_05990"
     CDS             <1..299
                     /locus_tag="VIV63_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY6124181.1"
                     /translation="KTMSDIGNLNSAFHLALIVPHQQPVTREVLSRLHTLSQRYVRMH
                     LLPVGRVRRATREHNALYEAWAEGDSRQARQLTAQHIEETRDELAQFLEGDSRL"
     gene            complement(359..742)
                     /locus_tag="VIV63_05995"
     CDS             complement(359..742)
                     /locus_tag="VIV63_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124182.1"
                     /translation="MDRQPITIAIVDDDLGIRQALERMLNIAGFRVLAFGSGEEFTAS
                     VPTTEADCAVIDVDLGTMCGLDLARHPAVIAAKLPLIFVSGSADEAVRGRAIAVGCVE
                     FLRKPFTPIELLDAIGRATLELPIP"
     gene            905..1924
                     /locus_tag="VIV63_06000"
     CDS             905..1924
                     /locus_tag="VIV63_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEY6124183.1"
                     /translation="MKRNTRLPRALGVMTRLAFAHARAHGIATAPLLARAGLSRRQIE
                     DPKVRIRVRDQVEFLNLVATAAKDDVLGFHLAHHCELRTVGLYFYVLASSETVKDVCE
                     RGARYSGLINEGVTQHLVDDAQIGIRTQFTSFSRIDDRHQVEFWTVALVRMLRQFTGT
                     RLIPRLVSFMHPRKRAVSEMCRYFGCEVQFGAAVDEVIFEKGAGKLPVVNSDPHLNRV
                     LVAICEDTLTRDVASRASVRTRVENEIATLLPHGKARAALVATRLGMSQRTLARHLAD
                     QGSSFSALLARIRRDLASRYLEDESLPISQIAWLLGFQDPGAFSHAFKRWHGTAPRHA
                     AVRVR"
     gene            complement(2009..2093)
                     /locus_tag="VIV63_06005"
     tRNA            complement(2009..2093)
                     /locus_tag="VIV63_06005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2057..2059),aa:Leu,seq:cag)
     gene            2182..>2921
                     /locus_tag="VIV63_06010"
     CDS             2182..>2921
                     /locus_tag="VIV63_06010"
                     /inference="COORDINATES: protein motif:HMM:NF036509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY6124184.1"
                     /translation="MKRRNGKSRGSQENPRLAHKGGRRPAAPAGSSAQGHGRVAEGIV
                     SANRAGFGFVRIEGQDEAVFLPPPQMAGVMHGDRVRVSVERGRDGRYSGRLERILEHA
                     TKAFVGTLEVHGRTAFVTAADRRIGMRCMVPPEELAGARHGDWVIAAVTRYAGSGASP
                     QARVVRRLDPEKPVELACESAIARFDLPREFSPEAVREAEAYGNEVDPAEAARRVDLR
                     DLPLVTIDGEDAKDFDDAVYCEPHAKGFR"
BASE COUNT          532 a          925 c          961 g          503 t
ORIGIN      
        1 ccaagacgat gagcgacatc ggcaacctca acagtgcctt tcacctggcg ctcatcgtgc
       61 cgcaccagca gccggtcacg cgcgaagtgc tgagccggct gcatacgctg tcccagcgtt
      121 acgtgcgcat gcatctcctg ccggtaggcc gcgtgcggcg cgccacgcgc gaacacaatg
      181 cattgtacga agcctgggca gaaggggatt cccgccaggc gcggcagctc acggcgcagc
      241 acatcgagga aacccgcgac gagctggcac agtttctcga gggcgacagc cggctatagg
      301 ccctttgtcc cattgagctg gcggcgcccc aggcgggtag atagagaatg gcaccacgtc
      361 acggaatcgg gagttccagt gtcgctcggc caatggcgtc gagcagctcg atgggcgtga
      421 acggcttgcg caggaactcg acgcagccga cggcgatcgc ccggccgcga actgcttcgt
      481 ccgcgctgcc gctgacgaag atgagcggaa gtttggcggc aatgaccgcg ggatggcggg
      541 ccaggtcgag cccgcacatg gtgccgagat cgacgtcgat gaccgcgcaa tcagcttcgg
      601 tggtgggcac gctggccgtg aattcctcgc cgctgccgaa tgcgagcacg cgaaaaccgg
      661 cgatgttgag cattcgttcc agtgcctgcc gtatcccgag atcgtcgtcg actattgcga
      721 tcgtgattgg ttgtctgtcc atgcctgtct ggtcgggtac tcggccgcct ggtccgatag
      781 tgagttccct cggcgcgagt cccggttccc ggttgaagtt gcgcgtctta tacgggcggc
      841 gtacatcagc tcgcttgttg cccccagcca gaaacttgtc gcttcgcgcc aggggccttt
      901 ccccgtgaaa cgaaacacac gactgccgcg cgcgctcggt gtcatgacgc gtctcgcgtt
      961 cgcacatgcc agggcccatg gcatcgccac ggcgccgctg ctcgcgcgcg ccggattgtc
     1021 gcggcggcag atcgaagacc cgaaggtgcg tatccgcgta cgcgatcagg tcgaattcct
     1081 gaacctggtc gcaacggcgg cgaaagacga tgtgctcggg tttcacctgg cgcatcactg
     1141 cgagctgcgc accgtgggac tctatttcta tgtgctcgcg tcctccgaga ccgtgaagga
     1201 cgtttgcgag cgcggcgcgc gttacagcgg cctgatcaac gaaggggtca cgcaacacct
     1261 cgtcgacgat gcgcagatcg gcatccgcac acaattcacc agcttcagcc gcatcgatga
     1321 ccggcaccag gtggagttct ggaccgtggc gctggtgcgc atgctccggc agttcaccgg
     1381 cacgcgcctg attccacgac tcgtgagttt catgcacccg cgcaagcgcg cggtcagcga
     1441 gatgtgccgc tacttcggtt gcgaggttca attcggcgcc gccgtcgacg aagtgatctt
     1501 cgaaaaaggc gcgggcaagt tgccggtcgt gaactccgat ccgcatctca atcgcgtgct
     1561 ggtcgcgatc tgcgaagaca ctctgactcg cgacgtcgca agccgtgctt ccgtgcgcac
     1621 gcgtgtggag aacgaaatcg ccacgctcct gcctcatggc aaggcgcgcg cggcactggt
     1681 ggcgacccgg ctaggcatga gtcaacgaac cctggcgaga catctcgccg atcagggcag
     1741 cagtttttcc gcgctgctgg ccaggatccg gcgcgacctc gcgagccgct acctggaaga
     1801 cgaaagcctg ccgatcagcc agatcgcgtg gttattggga tttcaggatc ctggcgcctt
     1861 ctcgcatgcg ttcaaaagat ggcacggaac ggcgcctcgc cacgccgcgg ttcgcgtgcg
     1921 ctgactcaac tgtgcgtaga atccgcgcgg tcgcgcccgg agcttagggg cgctggcggt
     1981 cgaagaccgc gtaatccatt gatttgattg gtgcccggga gaggactcga acctccacga
     2041 tgttactcgc tagtacctga aactagtgcg tctaccaatt ccgccacccg ggcagggtgc
     2101 gaaggacggc gaaaggtacg caccactttg taagctgtca atcgatgggg gaaggccccc
     2161 tctcagacca ggaagaaata gttgaaaaga agaaacggaa agtcacgcgg aagccaggaa
     2221 aatccgcgcc tcgcgcacaa gggcggccgc cgtccggcag cgccggctgg ctcgtcggcc
     2281 caaggtcacg ggcgggtcgc cgaaggcatc gtgagcgcca accgcgcggg ctttggcttc
     2341 gtgcgcatcg aaggacagga cgaagcggtg ttcctgccgc cgccgcagat ggccggtgtc
     2401 atgcacggcg accgcgtacg cgtatcggtc gagcgtgggc gtgacggacg atattcgggc
     2461 cgcctggaaa gaattctcga acacgccacc aaggctttcg tcggcacgct cgaagtacac
     2521 ggccgcacgg cgttcgtgac cgcggcggat cggcgcatcg gcatgcgctg catggttccg
     2581 ccggaagaac tcgcgggcgc gcgccacggc gattgggtga tcgcggccgt cacgcggtat
     2641 gcgggttcgg gtgcatctcc gcaggcgcgc gtcgtcaggc ggctggatcc cgagaagccg
     2701 gtggagctcg cgtgcgagtc cgccattgcg cgcttcgacc tgccgcgcga attctcgccc
     2761 gaggcggtcc gcgaagcgga agcctatggc aatgaagtcg atcccgccga agccgcgcgg
     2821 cgcgtggacc tgcgcgacct gccgctggtc accatcgacg gcgaagacgc caaggacttc
     2881 gacgacgcgg tctattgcga accgcacgcc aaaggtttcc g
//
LOCUS       DATDEU010000245         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1210830, whole genome shotgun sequence.
ACCESSION   DATDEU010000245 DATDEU010000000
VERSION     DATDEU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210830"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VIV63_06015"
     CDS             complement(<1..211)
                     /locus_tag="VIV63_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124185.1"
                     /translation="MTITIGAALGGCHVLPEYQFARTLPLSRSRTPVNIEGAHGAVSR
                     AEGARVTRKLADTGDTNLLDYHLAAM"
     gene            513..812
                     /locus_tag="VIV63_06020"
     CDS             513..812
                     /locus_tag="VIV63_06020"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEY6124186.1"
                     /translation="MDPAVEELNRIVEQAEALLSSLGNEGSEAAEAVRLRVSQTLNQA
                     KSKLAATASQAEEVAVSLADRADGYVRSNPWQAIAIAALLGSVVTLLIAKSTNRT"
     gene            812..1342
                     /locus_tag="VIV63_06025"
     CDS             812..1342
                     /locus_tag="VIV63_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124187.1"
                     /translation="MEEPTDAPAEESAEMEEEPGTARALTLHVLRMIETRMDAAGIAL
                     QSEIHSFTARLQFRMLAAAAFFLALWGGIVLLAIALPPHLRIPVLSAVVAAFVIGGIW
                     AYLAANRAVSSHDVGSMTWFLDGLKQDVDVLSRSLAQSRQQRNPQAQAPSPPPPEPPP
                     AAANETTRSDPNDLAA"
     gene            1326..1565
                     /locus_tag="VIV63_06030"
     CDS             1326..1565
                     /locus_tag="VIV63_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124188.1"
                     /translation="MISPLERARLLDAEMRLQRAWLELSLRELTGAARDSPWAALKPL
                     KLGKMGLSFLKHRSLWLAGLSLLVNFYRRHTHKEK"
     gene            1567..2148
                     /locus_tag="VIV63_06035"
     CDS             1567..2148
                     /locus_tag="VIV63_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY6124189.1"
                     /translation="MKTNLKAHVEPASGESAFISDLSALRARAREQMMKGAVTPSYPE
                     ADRKAIVELLNTALATEIVCVLRYKRHYYAAHGIRAKFVSAEFLEHAREEQAHADQIA
                     ERIVQLGEDPDLNPATLLSRSHSEYDQSTSLRQMLEADLFAERIAIESYREMINFIGP
                     RDTTSRRMLESILAQEEEHADDLVDLLEDLPKD"
     gene            2150..>2616
                     /locus_tag="VIV63_06040"
     CDS             2150..>2616
                     /locus_tag="VIV63_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6124190.1"
                     /translation="MQQDDELLGPELEARIAARTEELSTLSTHLLRKTEMERSALAKE
                     LHDELGGLITAAKMDMSWLSARLGGALDAESREKLESVMQMLNQAMMLKRRVVENLRP
                     SLLDHFGLAVAMRSHFDENCARAGIECVSTLPDEPLELDVVTQLSLFRVAQEAL"
BASE COUNT          485 a          833 c          862 g          436 t
ORIGIN      
        1 gcatcgccgc gaggtgatag tcgaggaggt tggtgtcgcc cgtgtcggcg agcttgcgcg
       61 tgacgcgcgc gccttccgcg cggctgaccg cgccgtgggc gccttcgatg ttgaccggtg
      121 tgcgcgatcg cgacagcggc agggtgcgcg cgaactggta ttcgggaagg acgtggcatc
      181 cgccgagcgc ggcgccgatc gtgatcgtca cgagccaccc cgcccaccgg ccgtgggacc
      241 ggtgggcgga tggcccggtg gtcgcatgac gcgaattcat ttcgagggga gggctcgtcg
      301 cgtcactcgt ccacttctcc actagacagg cgcgagtatc gctcccgcac gcgttgcggc
      361 gcgcccggca ggtgtgccat cgtgacgtcg gtagagtcct acaacccgtg cacagcaaat
      421 ccgaatgtga cccgatgaga cagggtccat tgtgtgatgc ataaggagca acaatgcctg
      481 acaccgccaa aaaaccagag ccaaacaaac ccatggatcc agccgtcgaa gaattgaatc
      541 gcatcgtcga gcaggccgag gcgctgctga gctcgttggg caacgagggc agtgaagccg
      601 ccgaggcagt acgcctgcgc gtcagccaga cgctgaacca ggccaagtcg aaactcgcgg
      661 cgacggccag ccaggccgaa gaggtcgcgg tgtcgctggc cgatcgcgcc gatggatacg
      721 tgcgcagcaa cccgtggcag gccatcgcca tcgcggcgtt gttaggcagt gtcgtcacgc
      781 tgctcatcgc caagtcgacg aatcgcacgt gatggaagaa ccgacggacg cgccggcgga
      841 ggaatccgcc gaaatggagg aagagccggg gactgcgcgc gcgctcacgc tgcatgtgct
      901 gcgcatgatc gagacgcgca tggacgcggc gggtattgca cttcaatcgg agatccactc
      961 gttcactgcg cggctgcagt tccgcatgct cgcggccgct gcgtttttcc tcgccttgtg
     1021 gggcggcatc gtcctcctgg ccatcgcctt gccgccgcat ctgcgaattc cggtcctttc
     1081 cgcggtggtg gctgccttcg tgatcggtgg catctgggcc tacctcgccg cgaatcgggc
     1141 ggtgtcgtca catgacgtgg gctccatgac ctggttcctc gacggcctga agcaggacgt
     1201 cgacgtgttg tcgcgcagcc tcgcgcagtc gcggcagcag cggaatccgc aggcccaggc
     1261 gccgtccccg ccgccgccag agccgccgcc ggcggccgcc aacgaaacca cccggagcga
     1321 tcccaatgat ctcgccgctt gaacgcgccc gactgctcga tgcggagatg cgcctgcagc
     1381 gcgcgtggct cgaactttcc ctgcgggagt tgaccggcgc cgcgcgcgac tcgccctggg
     1441 ccgccctgaa gccgctgaag ctcggcaaga tggggctttc gtttctgaag catcgatcgc
     1501 tgtggctggc gggactgagc ctgctggtga acttctaccg gcgccacacg cacaaggaga
     1561 aatgacatga aaaccaatct caaagctcac gtggagcccg cgtccgggga atcggccttc
     1621 atctccgacc tgagcgcgct gcgcgcgcgg gcccgcgagc agatgatgaa aggcgcggtc
     1681 acgccgagtt atcccgaggc cgaccgcaag gccatcgtcg aactactgaa caccgcgctc
     1741 gcgacggaaa tcgtctgcgt gctgcgttac aagcgccact actacgcggc gcatggcatt
     1801 cgcgcgaaat tcgtatcggc cgagttcctc gaacacgcca gggaagaaca ggcgcacgcc
     1861 gaccagatcg ccgagcgcat cgtgcagctc ggggaagacc ccgacctgaa tcccgcgacg
     1921 ctgctgtcac gttcgcattc ggaatacgac cagtcgacct cgttgcgcca gatgctggag
     1981 gcggatctgt tcgccgagcg tatcgccatc gaaagttatc gcgaaatgat caacttcatc
     2041 ggcccgcgcg acaccacgtc ccgccgcatg ctcgagtcca tcctggcgca ggaagaagag
     2101 catgctgatg acctcgtgga tcttctcgag gacctgccga aggattgaca tgcagcaaga
     2161 cgacgaacta ctcgggccgg agctcgaggc gcgtattgct gcgcgtacgg aagaactgtc
     2221 cacactgtcg acgcacctgt tgcgcaagac ggagatggag cgctcggcgc tcgccaagga
     2281 actgcacgac gaactgggcg gattgatcac ggccgccaag atggacatgt cgtggctgtc
     2341 ggcccggctc ggtggtgcgc tcgatgccga gagccgcgag aaattagagt cggtgatgca
     2401 gatgctcaac caggccatga tgttgaagcg gcgcgtggtc gaaaacctgc ggccgtcgct
     2461 gctcgatcac ttcggcctgg cggtggcgat gcgcagccat ttcgacgaaa actgcgcgcg
     2521 cgccggcatc gagtgcgtgt ccacgcttcc cgatgaaccg ctggagctcg atgtggtgac
     2581 gcagctcagc ctgtttcgcg tggcgcagga agcgct
//
LOCUS       DATDEU010000246         3522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1502909, whole genome shotgun sequence.
ACCESSION   DATDEU010000246 DATDEU010000000
VERSION     DATDEU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502909"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VIV63_06045"
     CDS             <1..480
                     /locus_tag="VIV63_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY6124191.1"
                     /translation="RAGDVYWDYSDNVRYRYDEIEDRFGVAPERFADFLALMGDSVDN
                     IKGVPGVGSKTASALMKVFVSLEEIYDNLDRVADIPVRGAGKLAAKLVEHREAAFFAR
                     RLTEIACNMPLEVGRNDLVRRAPDVSALTSFFDRHNFGPMLRRQAERLALLPPAAAA"
     gene            complement(496..1356)
                     /locus_tag="VIV63_06050"
     CDS             complement(496..1356)
                     /locus_tag="VIV63_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY6124192.1"
                     /translation="MRTACCLLLIALTPATALAWGDGCKLRADRAAGIEAKGVEKVII
                     RTGAGDMKVVGRGNAVRIEARGVACAAKQELLDASQISVRREGNVVYVEATLPQDDDN
                     WSWGNNDYAYIDIGIALPSSIPVEATDSSGDATFEDIHALNLQDSSGDLNLNRIAGLV
                     DVGDSSGDIEIESAGSVRVRDSSGDIDIDDVRADIEVVLDSSGDIEIAKVGGSVKVQQ
                     DSSGEIRIEDVKGSVNIDSDSSGDIYAGIVGGDFTVSEDSSGSINHKSITGKIHVPDN
                     KRDDDDRDVE"
     gene            1474..>3522
                     /gene="acs"
                     /locus_tag="VIV63_06055"
     CDS             1474..>3522
                     /gene="acs"
                     /locus_tag="VIV63_06055"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY6124193.1"
                     /translation="MSSENIQSVLIEERSFPPSPDFTASARLKPADVAALRREAAEDY
                     LGFWARQARTELRWHKPFTVTLDDSQAPNYRWFTDGELNVSYNCLDVHLEERGHKTAV
                     TFVGEPGDVRHVSYRELHADVCRFANALLAQRVKAGDRVIIYMPLTPEAVVAMHACAR
                     IGAIHSVVFGGFSALSLRDRIEDAGAVMLITADGGFRGGKIVELKEAADKALSQGCKT
                     IRSVIVLKRTAHEVPMKGGRDVWWHDAVEGQSPVCEPHWVDAEHPLFLLYTSGSTGKP
                     KGIQHASAGYLLGAKLTSKWVFDLNEEDVFWCTADIGWVTGHSYVAYGPLAAGATIVL
                     YEGAPTTPHPGRFWSICESQGVTIFYTAPTAIRALMKLGDDIPARYDLTRLRLLGSVG
                     EPINPEAWMWYHENVGRMRCPIVDTWWQTETGAIMISPLPGVTATKPGSCTQPLPGID
                     ATIVDARGNEVQGADAGGYLVVRKPWPSMLRTIWGDNERYLKTYWETFQNRFYVAGDS
                     AHRDKDGFFWIMGRIDDVLNVAGHRLGTMEIESALVAHPKVAEAAVVGKQHEIKGESV
                     FAFVVLRGDRPAAEKIEALIKTLRDWVTEQLGAIARPDDIRFSDNLPKTRSGKIMRRL
                     LRAIARNEDITQDLSTLENPGIIDQLRGGETPVKAAASKKVIPKKAAPKKKAVTRKKA
                     A"
BASE COUNT          637 a         1163 c         1098 g          624 t
ORIGIN      
        1 cgcgccggcg acgtgtactg ggactactcc gacaatgtcc gctatcgcta cgacgaaatc
       61 gaggaccgct tcggcgtcgc gcccgagcgt ttcgcggatt tcctcgcgct catgggcgac
      121 agcgtcgaca acatcaaagg cgtgccgggt gtcggctcca agaccgcgtc ggcgctgatg
      181 aaagtgttcg tgtcgctcga ggagatctac gacaacctag atcgcgtggc ggacatcccg
      241 gttcggggcg cgggcaaact cgccgcgaag ctcgtcgagc acagggaggc cgccttcttc
      301 gcgcgccgcc tcaccgaaat tgcctgcaac atgccgctgg aagtgggacg caacgacctc
      361 gtgcgccgcg cccccgatgt gagcgcgctc acctcattct tcgaccggca caatttcggc
      421 ccgatgctgc gccgtcaggc ggagcggctg gccctgctgc cgcccgctgc ggctgcctga
      481 ggcctgcctc tttttctatt ccacgtctcg atcgtcgtca tcgcgcttgt tgtccggcac
      541 gtgaatcttg ccggtgatcg acttgtggtt gatggaccct gagctgtctt cgctcaccgt
      601 gaaatcgccg ccgacgatcc ccgcgtagat gtcgccggag ctgtcggaat cgatgttgac
      661 gctgcccttc acgtcctcga tgcgaatctc ccccgagctg tcctgctgca ctttcacgct
      721 gccgccgacc ttggcgatct cgatgtcacc cgaactgtcg agcacgactt cgatgtcggc
      781 gcgcacatcg tcgatgtcga tgtcgcccga gctgtcacgc acacgcacgc tgccggcgct
      841 ttcgatttcg atgtcgcccg aactgtcgcc gacgtcgacg aggccggcga tgcggttgag
      901 gttcaggtcg cccgagctgt cctgcaggtt gagcgcatgg atatcctcga aggtggcatc
      961 gccgctcgaa tcggtggctt ccacggggat gctggacggc agggcaatgc cgatgtcgat
     1021 gtaggcgtag tcattgttgc cccaggacca gttgtcgtcg tcctgcggca gcgtggcttc
     1081 gacataaacc acgttgcctt cgcggcgaac actgatctgc gaggcgtcga gaagctcctg
     1141 cttcgcggcg caggcgaccc cacgcgcttc gatgcgaacc gcgtttccgc gcccgaccac
     1201 cttcatgtcg cccgcgcccg tgcggatgat caccttttcg acgcccttgg cctcgatgcc
     1261 cgcggcgcga tcggcccgaa gcttgcaccc gtccccccag gcaagggcgg tggcgggggt
     1321 cagggcgatc aacagcagac aacaggcggt acgcatggga attctccgga aatgctgggc
     1381 ggctatggtt aagactgacg agaaggcgtt tcggtttaca ggaccgaaca attgatacgg
     1441 tatccttcgg gccgcccctg aagcagccag aacatgagca gcgaaaacat ccaatccgtt
     1501 ctcatcgaag aacgcagctt tcccccttcc cccgacttca ccgccagcgc gcgcctcaaa
     1561 cccgcggacg tcgccgcgtt gcggcgcgaa gccgccgaag attacctcgg attctgggcg
     1621 cgccaggcgc gcaccgagct gcggtggcac aagccattca ccgtcacgct cgacgattcg
     1681 caggcgccga actaccgctg gttcaccgac ggcgaactca acgtttcata caactgcctc
     1741 gacgtgcacc tcgaagagcg cgggcacaag acggcagtga ccttcgtcgg ggagccgggc
     1801 gacgtacgcc acgtctccta ccgcgaactg catgccgacg tctgccggtt cgccaacgcg
     1861 ctgctggcgc agcgcgtgaa ggcgggcgat cgcgtcatca tctacatgcc gctgactccc
     1921 gaggcggtcg tcgcgatgca cgcctgcgcg cgcatcgggg caatccactc cgtggttttc
     1981 ggcggtttct ccgcgttgtc gctgcgcgat cgcatcgagg acgccggcgc ggtgatgctc
     2041 atcaccgctg atggcggatt ccgtggcggc aagatcgtgg agctcaagga agcggccgac
     2101 aaggcgcttt cgcagggctg caagaccatt cgcagcgtca tcgtgctcaa gcgcaccgcg
     2161 cacgaagtgc ccatgaaagg ggggcgcgat gtctggtggc acgacgccgt cgaaggccag
     2221 tcgccggtct gcgaaccgca ctgggtcgac gccgaacatc cgctgttcct gctgtacacc
     2281 tccggctcca ccggcaaacc caagggcatc cagcacgcga gcgcgggcta tctactcggc
     2341 gcgaaactca ccagcaagtg ggtgttcgat ctcaacgaag aagacgtgtt ctggtgcacg
     2401 gcggacatcg gctgggtcac cggccacagt tacgtggcct acgggccgct ggccgcgggc
     2461 gccaccatcg tgctgtacga aggcgcgccg acgactccgc atccgggacg cttctggtcc
     2521 atctgcgagt cgcagggcgt gaccatcttc tacaccgcgc ccaccgcgat ccgcgcactc
     2581 atgaaactcg gcgacgacat ccccgcacgc tacgacctca cgcggctgcg gctgctcgga
     2641 tcggtgggcg agcccatcaa tccggaagcc tggatgtggt accacgagaa cgtcgggcgc
     2701 atgcgctgcc cgatcgtcga cacctggtgg cagacggaaa ccggcgccat catgatttct
     2761 ccgctccccg gcgtcaccgc caccaaaccg ggttcttgca cgcagccttt gcctggcatc
     2821 gacgcgacta tcgtcgatgc acgcggaaac gaagtgcagg gcgcggatgc cggcggttac
     2881 ctcgtggtcc gcaagccctg gccgtcgatg ttgcgcacga tctggggcga caacgagcgt
     2941 tacctcaaga cctactggga aactttccag aaccgctttt acgtggcggg cgactcggcg
     3001 catcgcgaca aggatggctt cttctggatc atgggccgca tcgacgacgt gctgaacgtc
     3061 gccggccatc gcctgggcac catggagatc gaatccgcgc tcgtggcaca tccgaaagtc
     3121 gcggaagccg cggtggtcgg caagcagcac gagatcaagg gtgaatcggt gttcgccttc
     3181 gtggtgttgc gcggcgaccg gcccgctgca gaaaagatcg aagcgctgat caaaacgctt
     3241 cgtgactggg tgaccgagca gttaggcgcc atcgcgcgac cggacgacat ccgtttctcg
     3301 gacaacttgc ccaagactcg ttcgggcaag atcatgcgcc gcctgctgcg cgccatcgcg
     3361 cgcaacgaag acatcacgca ggatctctcg acgctcgaga atccggggat catcgaccag
     3421 ctgcgcggcg gcgagacgcc tgtgaaggcc gcggcttcca agaaagtgat tccgaagaaa
     3481 gcggcgccga agaaaaaggc cgtcaccagg aaaaaggcgg cg
//
LOCUS       DATDEU010000247         6365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_189527, whole genome shotgun sequence.
ACCESSION   DATDEU010000247 DATDEU010000000
VERSION     DATDEU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189527"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VIV63_06060"
     CDS             complement(<1..513)
                     /locus_tag="VIV63_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124194.1"
                     /translation="MSAKPDYVVSKLTRPAGGAATGGFVINLTSSTTPMALSQPKDPT
                     LSQYTFFVSRRLEDGRERFRLHMGYFESMQAAEEMLLVVRDVYPGAWASEAPGKKLKA
                     NNPAPAPAAAAPAPAARAPAPAPVQQPKPAAAAPAPVQQPKPAAATPTPAPVAPAPVA
                     PAPVAAAPGGG"
     gene            complement(738..1127)
                     /locus_tag="VIV63_06065"
     CDS             complement(738..1127)
                     /locus_tag="VIV63_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY6124195.1"
                     /translation="MRNRGETLADFALRLQGTPYRYGGATRDGFDCSGLVFYAHRQLG
                     LVVPRTSSDQAEEAQGIKPRKLRRGDLVFFKIGSRKVNHVGIYIGDHRFVHAPGAGKP
                     VTINSLDDEFYSERFSSAGRFWNRLPN"
     gene            complement(1294..3834)
                     /locus_tag="VIV63_06070"
     CDS             complement(1294..3834)
                     /locus_tag="VIV63_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6124196.1"
                     /translation="MSAHPYNSVLRSGLTRRLWVIITLAMLVPVSFSVLSRWFDAEES
                     RASLQNQELTALSRDMASTLLFSSRVVPPDFATGLDGRYLVVLDGTGSARYRSSPVPD
                     ELVELFGRRAPESGQAPSGTTILAWYAAGREWRGAVTYLPPGVPGNPLSSDTIVVFAP
                     EATFGSTTSGLVPTALGLLALTILISFAVAAIVSERYLPPLRALERGLARLRERRFET
                     LPRSTVEEFTTLEREFNATSISLQRDWRAFEVLGEVDRALLAASEIDRALDVVLPKFR
                     ELTRSQCVGVILLDPTAHAHGRLFMAALGADELPVQRVTFDSAMIATVREAPEGLTVA
                     RIEESRHAFLIAMRDVGAEFFWLWPVVDEERLSALLVVGYHGEPSRDPAVADYGAAFA
                     ERLRAALSNSARDERLYRQAHFDPLTQLPNRALFRDRLAQELAAAATGLTRGALMYVD
                     LDHFKRVNDTLGHSAGDQLLSVVAHRLKACTKEGDTVARLGGDEFTVLLRNVGDAETA
                     RSIADRVIRSLALPANLGGRDYQMRASVGVTLFPDDGVDLEDVIRQADLAMYRAKAQG
                     RGRAIFFERDMVQRRASFTDSGLHRALRRRELSLFYQPQFSLADNRLCGVEALLRWQT
                     RYDGMKLPGEFIPAAEHSGLITDIGGFVLDSACGQFAEWSRAGIAPRMFSVNISLQQL
                     KDSNFVRMVQGALQKHGIAPASLELEVVESVLADAEVEEPLRAIADLGVKLALDDFGT
                     GYSSLKYLRRYPVHTVKLDRSFLDEIPQNDSAGALVESVITMAHTLGKRVTAEGVESG
                     GQLEFLRSRGCESAQGFYLARPMSAAALTEILESRRPGSEIDDAREAG"
     gene            complement(3960..4679)
                     /locus_tag="VIV63_06075"
     CDS             complement(3960..4679)
                     /locus_tag="VIV63_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY6124197.1"
                     /translation="MSTTPKIALVAGATGLVGGLLLKTLLDAPDYTRVYALTRRPFGK
                     EHPKLANRVVVFDRMAEQLKGLVAQDAFCCIGTTIADAGSQEAFRETDVDAVLLFARA
                     ARAAQATRFVVVSSVGASSQSKKFYLRTKGEMEEAVSDLGFVSVDILQPSLLLGPRKA
                     LRPIELAGAVLAPLINPLLTGTREPLRAISAETVAKAMLGAARRGARGTYRYTYSAIR
                     QLSEIRPPQPVGMQPPKKKPA"
     gene            complement(4707..5327)
                     /locus_tag="VIV63_06080"
     CDS             complement(4707..5327)
                     /locus_tag="VIV63_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEY6124198.1"
                     /translation="MLLVIDLIGVFVFALAGATAAVRQRLDLFGVLVLSFATATTGGI
                     LRDLLIGAVPPAAFADYRYLAAAVLAGLVTFTWHPLIERMRNPVRVFDALGLGLFAVA
                     GTQKALVAGLHPVMAALLGMLTGIGGGVMRDLLLTRVPVVFQSDIYALAALAGATIVV
                     LGDWLNWPAMPTAIAGTLLCFGLRILAMWFNWHMPRARGDDGPHKD"
     gene            5480..6316
                     /locus_tag="VIV63_06085"
     CDS             5480..6316
                     /locus_tag="VIV63_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124199.1"
                     /translation="MHVPSYPRVAALLLMLASFAGGAHAVEFDEKIRAPMVREPATLR
                     TQALSYAEKFKALHAASPQDVIRNRALTAERFDITWQIQQAIDTGRPLGDLSAIGLEK
                     KENGSYEIDFNANPEWDRPDFAFLTVLKHENQQAIDLVLTSRGFREADLATLKQYVST
                     HDLDQTVRSESLPLTLGFSKIVKKYDKLKRPVDDAAVLSYIYQREKLAAENGRRWAEG
                     VLNVLDAQRARILLSFMGEMSIKGIWAPSDQRAGIDEQLRLMRLPNFEQLVVAEAAGV
                     TP"
BASE COUNT         1211 a         2001 c         2100 g         1053 t
ORIGIN      
        1 cccccccccc ggagccgcgg ccacgggcgc gggagccacg ggcgcgggag ccacgggcgc
       61 gggagtcgga gtagcggctg cgggcttggg ctgttgcaca ggcgccggag cagcggccgc
      121 gggcttgggc tgttgcacag gcgcaggcgc cggagcgcgc gcagccggtg cgggagccgc
      181 agcggcagga gccggcgcgg gattgttggc cttcagtttc ttgccgggtg cttcgctggc
      241 ccacgcgcct ggatagacgt cgcgaaccac gagcaacatt tcttcggcgg cctgcatgga
      301 ttcgaagtaa cccatgtgca ggcgaaaacg ctcgcggccg tcttcgagac ggcggctgac
      361 gaaaaatgtg tattgagaga gcgtcgggtc tttcggctgc gacaacgcca tcggcgtagt
      421 cgaggacgtc aggttaatga caaatccgcc cgtcgcggct ccgccggcgg gccgcgtcag
      481 cttactgacg acgtagtctg gcttggcact catgtttaaa ccgttccccg aataatcgac
      541 accgaaccac gccccttccc gaacgcgatt ctttttcact caatttggag tgtgcgaacg
      601 taggcatata cggtcaaccg tcgaactgag cacgctgtcg cagaacgcag acctaaatat
      661 tcattccctt acggaaaatt cgacataacg gtgtggcaat ttttgctgcg aagcattaag
      721 gctgatcgtg agtcccgcta attcggcaga cggttccaga agcggccggc cgaagaaaac
      781 cgctcggaat agaactcgtc gtccagcgaa ttgatcgtca cgggttttcc cgcgccgggt
      841 gcgtgcacga accggtgatc accgatgtag atgccgacgt gattcacctt gcggctgccg
      901 atcttgaaga acacgaggtc gccgcggcgg agcttgcgcg gcttgatgcc ctgcgcttct
      961 tcggcttgat cgctcgaggt tcgcggcacc acgagcccga gctgccggtg cgcgtagaac
     1021 acgaggccgc tgcaatcgaa gccgtcgcgc gtcgcgccac cgtagcggta tggcgttccc
     1081 tgcagccgca gcgcgaaatc cgcgagtgtc tcgccgcgat tgcgcatgcc ggaggcgcca
     1141 ccggcgccgt cccattcgcg cgcgggttca ggcgctggct gagagcgggg ctcgggggtg
     1201 gacgagcagg cggtcagcag tagcagaatg aaaaagggga cagatctatt tttcagggac
     1261 gccagaccct gaaaaataga tctgtcccct ttttcatccc gcttcgcggg catcatcgat
     1321 ctcgctgccg ggccgccggg actcgagtat ctccgtgagc gcagccgccg acatgggtcg
     1381 cgccaggtag aagccctggg ccgattcgca gccgcgcgaa cgcaggaatt cgagctggcc
     1441 gcccgattcc acgccttcgg ctgtcacgcg ttttccaagc gtatgcgcca tggtgatgac
     1501 tgattcgacc agggcaccgg ccgaatcgtt ctgtgggatt tcatccagaa agctgcggtc
     1561 cagttttacg gtatgcacgg gatagcggcg cagatatttg agagacgaat atcccgtgcc
     1621 gaaatcgtcg agcgccagct tcacgccgag gtcggcgatg gcgcgcagcg gttcttcgac
     1681 ctcggcatcc gccagtaccg attcaaccac ctcgagttcg aggctggccg gcgcgatacc
     1741 gtgtttctga agcgcgccct gcaccatgcg gacaaaattg ctgtccttga gctgctgcaa
     1801 cgagatgttg accgagaaca tgcgcggcgc gattccggca cgcgaccact cggcgaattg
     1861 cccgcaggcg gagtcgagga cgaagccacc gatgtcggtg atgagccccg aatgctcggc
     1921 ggcgggaatg aactcgccgg gcagcttcat gccgtcgtaa cgcgtctgcc agcgcagcag
     1981 cgcctcgacg ccacaaaggc gattgtcggc gagcgagaac tgcggctgat agaacaacga
     2041 cagctcgcgc cggcgcagcg cgcgatgcag cccgctgtcc gtgaagctgg cgcgccgctg
     2101 aaccatgtca cgctcgaaga agatcgcacg tccgcgccct tgtgccttgg cgcgatacat
     2161 cgcgaggtcg gcttgacgga tcacgtcctc gagatccacg ccgtcatcgg gaaacagcgt
     2221 cacgccgacg ctggcgcgca tctggtagtc gcgtccgccg agattggcgg gcagcgccag
     2281 cgaacggatg acgcgatcgg caatgctgcg cgccgtttcg gcgtcgccga cattgcgcaa
     2341 caacacggtg aattcgtcgc cgcccagccg cgcgaccgtg tcgccctctt tcgtgcaggc
     2401 cttgaggcga tgcgcgacca ccgacagcag ttgatcgccc gcgctgtggc cgagcgtgtc
     2461 gttgacgcgc ttgaagtgat cgaggtccac gtacatgagc gcgccgcgcg taagtcccgt
     2521 ggccgccgcg gccagttcct gcgcgagccg gtcgcgaaac aacgcgcgat tcggcaactg
     2581 ggtgagcgga tcgaaatgcg cctggcggta cagacgttcg tcgcgcgcgc tgttcgacag
     2641 cgccgcacgc agccgctcgg cgaacgccgc gccgtagtcg gcgaccgccg gatctcgcga
     2701 tggctcgccg tgataaccga ccacgagcaa tgcactgagt ctttcttcgt cgaccaccgg
     2761 ccatagccag aagaattcgg cgccgacgtc gcgcatggcg atgaggaacg cgtgacggga
     2821 ttcttcgatg cgcgccaccg tgagcccttc gggcgcttcg cggaccgtgg cgatcatcgc
     2881 ggaatcgaag gtcacgcgct gcacgggcag ttcatcggcg ccgagcgccg ccatgaacaa
     2941 ccggccgtgt gcgtgcgcgg tcgggtcgag caggatcaca cccacgcact ggctgcgcgt
     3001 gagctcgcgg aacttgggca gcaccacgtc gagcgcgcga tcgatttcgc tggcggcgag
     3061 cagcgcgcga tcgacttcgc cgagtacctc gaaggcgcgc cagtcacgct gaagcgaaat
     3121 ggacgtggcg ttgaactcgc gttcgagcgt ggtgaattct tcgacggtgc tgcgtggcaa
     3181 cgtctcgaag cggcgttcac gcaggcgcgc gagcccgcgc tccagcgcgc gcagcggcgg
     3241 cagataacgc tcgctcacga tggcggcgac cgcaaaggaa atgagaatgg tgagcgccag
     3301 gagaccgagc gccgtcggca ccagccccga ggtggtggaa ccgaaggtgg cctcgggcgc
     3361 gaacaccacg atggtgtcgg acgagagggg attgcccgga acgcccggcg gcagataggt
     3421 gaccgcgccg cgccattcac gtcccgcggc gtaccacgcg aggatggtcg tgccgctcgg
     3481 cgcctgtccg gactccggcg cgcggcgtcc aaacaactcc acgagttcgt cgggcacggg
     3541 actgctgcga taacgcgcgg atccggtgcc gtcgagcacg acgagataac gtccatccag
     3601 tcccgtcgcg aaatccggcg gaacgacgcg cgagctgaac agcagcgtgg aagccatgtc
     3661 gcgcgagagc gcggtcagct cctgattctg caggctggca cggctttctt cggcgtcaaa
     3721 ccagcgcgac aacaccgaga aactcacggg cacgagcatc gccagggtga tgatcaccca
     3781 cagacggcgc gtgagtccgc tgcgcaagac tgagttgtac ggatgggcgc tcatcgactt
     3841 cccatgggtg tgcgttgcgc acctgcaacg caagccaggc attcctgttc ttgcgccgac
     3901 tttacggaat gcggaggcgc gcaaatgcgc gccgcgtcac ggttgcggtt gcaccgtatt
     3961 caggcaggct tcttcttggg cggttgcatg ccgaccggct ggggcgggcg aatctcggag
     4021 agctggcgga tcgccgaata ggtgtagcga taagtgcccc gcgcgccacg ccgggccgcg
     4081 cccagcatgg ccttggcgac ggtctccgcg gagatggcgc gcaagggttc gcgagtgccc
     4141 gtgagcaatg gattgatgag cggcgcgagg acggcgccgg cgagttcgat ggggcgcagc
     4201 gccttgcgcg gacctaacag cagactcggc tgcaggatgt ccacggatac gaaacccagg
     4261 tccgagacag cctcttccat ctctcccttg gtgcgcaggt agaacttctt cgattgcgag
     4321 ctcgcgccta ccgacgacac cacgacgaaa cgcgtggcct gcgccgcgcg cgccgcgcgc
     4381 gcgaacagca gtaccgcatc gacatcggtt tcgcgaaacg cttcctgcga accggcgtcg
     4441 gcgatcgtcg tgccgatgca gcagaaagcg tcctgtgcca ccagaccctt gagctgttcc
     4501 gccatgcggt cgaagacgac gacgcgattg gcgagcttcg gatgttcctt gccgaagggc
     4561 cggcgggtca gtgcatagac acgcgtgtag tccggtgcat cgagcagggt tttcagcagt
     4621 aatccgccga cgagaccggt tgcgccggcg accagcgcta tttttggtgt tgtgctcacc
     4681 gggcgaagct taacaagatt ctgcggtcaa tccttgtgag gaccgtcgtc gccgcgagct
     4741 cgcggcatgt gccagttgaa ccacatcgcg aggatgcgca gaccgaaaca cagcagcgtg
     4801 cccgcgatcg cggtcggcat cgccggccag ttcaaccagt cgcccagcac cacgatcgtc
     4861 gcacccgcca aagccgcgag cgcgtagatg tcggactgga acacgaccgg cacgcgcgtc
     4921 agcagaaggt cgcgcatcac gccgccacca attcccgtaa gcattcccag cagcgccgcc
     4981 atgaccggat gcagacccgc caccagcgcc ttctgcgtgc cggcgacggc aaacaacccg
     5041 agacccagcg cgtcgaaaac gcgcaccgga ttgcgcatgc gctcgatcag cggatgccag
     5101 gtgaacgtga cgagaccggc gagcaccgcc gcggccaggt accggtagtc ggcgaacgcg
     5161 gcgggcggca ccgcgccaat caggaggtcg cgcaggatgc cgccggtcgt cgcggtggcg
     5221 aacgacagca ccagcacgcc gaagagatcg agccgctggc gcacggccgc cgtcgcgcca
     5281 gccagcgcga agacgaagac accaatgagg tcgatgacca gtaacaccgg cgcattgtgc
     5341 accacggaac cgaaagtggg gcttcgcgtg gcttttgccg gctgctcacc cgaccgggtg
     5401 atgcggcgtc aggcgccttg attatgctgt cgcgtgggaa aggagtcctt acaaccttca
     5461 acgaacaagg agtgaataca tgcatgtccc tagctatcca cgcgtcgcgg cgttgctgct
     5521 gatgctcgcg agtttcgcgg gcggcgccca cgcggtggag ttcgacgaaa agatcagggc
     5581 gcccatggtc agggaaccgg ccacgctgcg cacccaggcg ctgtcatacg ccgagaagtt
     5641 caaggcgttg cacgccgcgt cgccgcagga tgtcattcgc aatcgcgcgc tgacggccga
     5701 acgcttcgac atcacgtggc agatccagca ggcgatcgac acgggccgac cgctgggtga
     5761 tctgtcggcg atcgggctgg agaagaagga aaacggcagc tacgagatcg acttcaatgc
     5821 gaacccggag tgggatcggc cggacttcgc gttcctcacg gtgttgaagc atgaaaacca
     5881 gcaggccatt gatctggtgc tgacaagccg cggctttcgc gaagcggatc tcgcgacgct
     5941 caagcagtac gtttcaacac acgatctcga ccagacggtc cgaagtgagt ccctgccgct
     6001 gacgctcggc ttcagcaaga tcgtcaagaa gtacgacaag ctcaagcgtc ccgtcgacga
     6061 cgccgcggtg ctctcataca tatatcagcg agagaaactg gcggccgaga acggccggcg
     6121 atgggcggag ggcgttctga acgtcctcga tgctcagcgt gcgcgaatcc tgctgtcctt
     6181 catgggagaa atgtccatca agggtatctg ggctcccagt gaccagcgcg ccggaatcga
     6241 cgagcaattg cgccttatgc gtcttccaaa cttcgagcag ttagtggtgg cggaagccgc
     6301 gggagtgacg ccatgaactt tctgatttcg gttcgtcgct gtgcagcggc actcgtactc
     6361 ggggc
//
LOCUS       DATDEU010000248         2988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_773920, whole genome shotgun sequence.
ACCESSION   DATDEU010000248 DATDEU010000000
VERSION     DATDEU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773920"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VIV63_06090"
     CDS             <1..581
                     /locus_tag="VIV63_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY6124200.1"
                     /translation="LRALTALNSSRGNDTVVVFLASHGLSDKQGNYYFVPRDARKPDI
                     DTLLDGGTLPTASSLVSWLDVSEALRKTSGRRLLIVDTCQARQISGHFADDPVIKRSA
                     SSRLAFILASKGDEESQEYASGRHGLFTYGLIEGLEPGVTVAEWFASAARTVDTLRDR
                     RIGPQTPQFMAPASLAGMQLMSKPPGGPRIPQ"
     gene            594..2111
                     /locus_tag="VIV63_06095"
     CDS             594..2111
                     /locus_tag="VIV63_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124201.1"
                     /translation="MSSTPIRRFLAWLAAPALFCLAGNALAQVDQMMPPVGGPGGGQF
                     AARCATDEILNGFELRVGDDVDAIRAICARATSPTSIASRSLHASSAGGPGGRVVQVV
                     CPDNTPAITSMAIGWEGVQTFIVNSVRLYCGIAAVNQWPAEFPTVAFDGPKVVAGTGF
                     GTTIAMGLLLETTRCAPGLVPVGIHGRSGKWLDAVGFVCGELRLDLSGYPKPAAPAKP
                     KLTPRGGAQFSIKVEWHRPAPANNIEWFSIDQWKNEEWVKLAKRIDARDLQTHIEFPD
                     VRSPDERQAFRVCSENAGGRACSPETWYYPFGKAQAEVSSTPIIAAAQQAAPAPEPPP
                     APITVANLQAMMARGLELTNKDPLAAELRRRLPDTAGRHGFDIGMGIWAGNTAPGPGK
                     QRYRAATLSESRYGFDIAAAFSLPRNKYAAQSNVGLSIARADRAVARERLSQDDPFFW
                     LGFDIASGIFGDPKAGAQGNTATGPGSQAIRNDLNAAGQRGFDAATAFHLARRYQ"
     gene            2122..>2988
                     /locus_tag="VIV63_06100"
     CDS             2122..>2988
                     /locus_tag="VIV63_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124202.1"
                     /translation="MKMKFRHFGAWTLTMLGIAGTAHGQADQLLPPTGGKGGAQYFAR
                     CALGEILNGVELRTGDDVDAIRPICAVAYSKAVIGPRIPYPQSFGGTGGVVTRLVCPD
                     NAPAIAGMFVASEGEDTEVVNNIWLYCSEALPTLAKPDYPSARFDGEEIGRTRDGWLA
                     RLLAFPAKTIFLDPHMHHCPAGLVPVGINGRSGIWLDSLGLTCGVLHIAPPDPNAVKS
                     IGRVGSPSAPRPARHICDAARDARARNSPAAPNLEAQCRAAPPPVTPPATPQSAPAPA
                     AITAADMQMVMAR"
BASE COUNT          521 a         1046 c          922 g          499 t
ORIGIN      
        1 tgcttcgtgc gctgacggca ctcaactcgt ccaggggcaa cgacaccgtc gtcgtgttcc
       61 tcgcatcgca tggcctgagc gacaaacagg gcaactacta tttcgtgccc cgtgacgcgc
      121 gcaagccgga catcgacacg ctgctcgacg gcggcacgct gcccaccgcg tcgtcactcg
      181 tgagctggtt agacgtatcc gaagcgctgc gtaaaacgtc cggccgccgc ctgctcatcg
      241 tcgacacctg ccaggcgcgg cagatctccg ggcacttcgc cgatgatccc gtcatcaagc
      301 gttcggcgtc gtcgcggctt gcgttcatcc tggcgtccaa aggcgatgag gagtcgcagg
      361 agtacgcttc cggccggcac gggcttttca cgtatggcct gatcgaggga ctcgagcccg
      421 gcgtcacggt cgccgagtgg tttgccagcg ccgcgcgcac cgtcgacacg ctgcgagacc
      481 ggcgcatcgg cccccagacg ccgcagttca tggcgcccgc atcgctcgcg ggcatgcagc
      541 tcatgtccaa accacccggc ggtccgcgta ttcctcaatg aggagtaccc gacatgagtt
      601 ccacgccgat ccgccgattc cttgcttggc tcgccgcgcc ggcattgttt tgcctcgccg
      661 gtaacgcgct ggcgcaagtc gatcagatga tgccgcccgt cggtggcccc ggcggcggcc
      721 aattcgcggc gcgctgcgcc acggacgaaa ttctcaacgg attcgaactg cgcgtgggcg
      781 acgacgtcga tgcgattcgc gcgatctgcg cccgcgcgac atcacccacg tccatcgcgt
      841 cgcgcagcct gcatgccagc agcgccggtg gtccgggcgg acgagtggtt caagtcgtgt
      901 gcccggacaa tacgcccgcg atcaccagca tggcgatcgg gtgggagggc gtgcagacct
      961 tcatcgtcaa tagcgtccgc ctgtattgcg gcatcgccgc cgtcaatcaa tggcccgcgg
     1021 agtttcccac cgtggccttc gacggtccca aggtcgttgc tggcactggc ttcggcacca
     1081 ccatcgcaat ggggctgctt ctcgaaacta cccggtgcgc gcccgggctc gtgccggtcg
     1141 gtattcatgg ccgctcgggc aaatggctgg acgcggtcgg cttcgtctgc ggcgaattgc
     1201 gcctggatct gtccgggtat ccgaagcccg ccgcgcccgc caaaccgaaa ctgacgcctc
     1261 gcggcggcgc ccaattcagc atcaaggtgg aatggcaccg gccggcaccg gctaacaaca
     1321 tcgagtggtt ctcgatcgac cagtggaaaa acgaggagtg ggtgaagctc gcgaagcgca
     1381 tcgatgcgcg cgacctgcag acccacatcg aatttccgga cgtgcgcagc cccgacgaac
     1441 gccaggcgtt ccgcgtgtgc tcggagaacg ccggcggcag ggcctgctcg cccgaaacct
     1501 ggtactaccc tttcggcaag gcgcaggcgg aggtgagcag cacacccatc attgcagcgg
     1561 cccagcaagc tgcgcccgct ccagaacccc cgcctgcgcc gatcaccgtg gctaatctgc
     1621 aagcgatgat ggcgcgcggt ctcgaactga ctaacaaaga tccgctcgca gccgaacttc
     1681 gccggcgcct gcccgatacc gccggccgtc atggcttcga tatcggcatg ggcatctggg
     1741 ccggcaacac ggcgcccggg cccggcaagc aacgttatcg cgctgccacg ctcagcgaat
     1801 cacgctacgg ctttgacatc gccgccgcgt tctcgctgcc acgaaacaaa tatgcggcgc
     1861 agagcaatgt cggcctctcg atagccaggg ctgatcgcgc cgtcgcgcgt gagcgcctct
     1921 cgcaggacga cccgttcttc tggctcggct tcgacatcgc cagtggcatc ttcggcgatc
     1981 ctaaggccgg cgcgcagggc aacactgcta cgggcccggg ctcacaggcc atccgcaacg
     2041 acctcaacgc agccggtcag cgcggcttcg acgccgcgac cgcattccat ctcgcgcgcc
     2101 gctaccagtg agaccaacgc catgaaaatg aaattcaggc acttcggagc atggacgttg
     2161 acgatgctcg gcatcgccgg cactgcgcac gggcaggcgg atcaactgct gccgcccacg
     2221 ggcgggaaag ggggcgcgca atatttcgcg cgctgcgcgc tcggagaaat cctgaacggc
     2281 gtcgagctgc gcaccggcga cgacgtcgac gccattcgcc cgatctgcgc ggttgcgtac
     2341 tcgaaggctg tcatcggccc gcgtattccc tatccgcaaa gcttcggcgg gacgggcgga
     2401 gtcgtcacga ggctcgtctg cccggacaat gcgccggcca tcgccggaat gttcgtggcg
     2461 tccgaaggtg aagacaccga ggtcgtgaac aacatctggc tgtactgcag tgaagcgctg
     2521 ccaaccctcg cgaaacccga ttatccgtcc gcgcgattcg acggcgaaga gatcggccgt
     2581 acgcgtgacg gctggctggc ccgactattg gcattcccgg caaaaaccat ctttctggat
     2641 ccgcacatgc accattgtcc cgccggcctc gtgcccgtcg gtatcaatgg acgctcgggc
     2701 atctggctcg attctttggg actcacctgc ggcgttctgc acatcgcccc gcccgatccg
     2761 aacgcagtaa agtccatcgg ccgcgtaggt agtccttccg caccgcgacc cgcaagacac
     2821 atctgcgatg ccgcgcgcga tgcgcgtgcc cgcaattcgc cggccgcgcc caatctggag
     2881 gcgcagtgcc gggccgcgcc gcctcctgtc acaccgcccg cgactccaca gtcggcaccc
     2941 gcgcctgctg caatcaccgc agccgacatg cagatggtca tggcgcgc
//
LOCUS       DATDEU010000249         2015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2086701, whole genome shotgun sequence.
ACCESSION   DATDEU010000249 DATDEU010000000
VERSION     DATDEU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086701"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VIV63_06105"
     CDS             <1..1174
                     /locus_tag="VIV63_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY6124203.1"
                     /translation="LITHRTKYMKNVRYRHIQSLHGQGLLLSEAADWRRQRVITQPAF
                     KPEAIDNSVGWMAEHVDHFLHRWDGYADRGEVFDVEPEFNRLAQLLSGRYLLGEPFAE
                     IADEFCEVAAAVKKHWPQAPRNIVRAFIPHSKELIARFDTAIAALDACILGFIQRHRP
                     TDFEGCGVLKRIVTASRAEGNEFDDKSLRDQLSTLFFAGHETSATGLCWIHWALSRHP
                     QFRQKMVAEVNEVLGSRLATAEDVHKLQYTGQLIDESLRLHSPIHSISRVAMVDNTVG
                     GFHIPAGATVAVSMYAIHRQAHLYPNPEVFDPTRFAPEQVATRHRFAYLPFAAGHRNC
                     IGAGQALVELKLIVSRIAQRFELDLAPGHKVEPAPGTTMFPRYGMKMKIRHIKAAR"
     gene            1171..1926
                     /locus_tag="VIV63_06110"
     CDS             1171..1926
                     /locus_tag="VIV63_06110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6124204.1"
                     /translation="MSVPADSRSEQARQFYNAAASTSTDFAFMNYGYAPLASDLDGTA
                     EPEKYCLQLYRRLLGNVDLRGKKVVEVSCGRGGGAAHVAATYQPASYLGIDISERNLS
                     LAMHRFPGVSSLSFQVGNAEALPLPDASFDALLNVEASHLYDNPARFFAETFRVLKPG
                     GTFFHVDLAWKDKDPPAMIAAAGFKIASVEDITKNVVLALDLDSVRREAIVMSFPENM
                     RNDFRDWSGVKGFRAYNRLQNGEWVYRITRGKR"
BASE COUNT          369 a          667 c          615 g          364 t
ORIGIN      
        1 gctgatcacg catcgcacga agtacatgaa gaacgttcgg taccggcaca tccagtcgct
       61 gcacggtcag ggactgctgc tctccgaggc cgccgactgg cgccgtcagc gcgtcatcac
      121 gcagccggcg ttcaagcccg aagccatcga caactccgtc ggctggatgg ccgaacacgt
      181 cgatcatttt ttgcatcgct gggacggcta cgccgatcgc ggcgaggtgt tcgacgtcga
      241 accggaattc aaccggctgg cgcaattgct gtcgggccgc tatctactcg gtgaaccgtt
      301 cgccgaaatc gccgatgaat tctgcgaagt ggccgcggcc gtcaagaaac actggccgca
      361 ggcgccgcgc aatatcgtgc gcgcgttcat cccgcattcg aaagagctga tcgcgcgctt
      421 cgacaccgcc atcgccgcgc tcgacgcctg catcctcggg ttcatccagc gccaccgccc
      481 caccgatttc gaaggctgcg gcgtgctcaa gcgcatcgtc accgcgagcc gtgccgaggg
      541 caacgagttc gacgacaagt cgctgcgcga ccagttgtcg acgctgttct tcgcgggtca
      601 cgagacctcc gccaccgggt tatgctggat ccactgggcg ctgtcgcgtc acccgcagtt
      661 ccgccagaag atggtggccg aggtcaacga agtgctcggc agccggctgg ccaccgccga
      721 agacgtgcac aaactgcaat acaccggcca gctcatcgat gagtcgctgc gcctgcattc
      781 gccgatccac tccatctcgc gcgtggctat ggtggacaac acggtcggtg gttttcacat
      841 cccggccggc gccacggtgg ccgtgtcgat gtatgcgatc caccggcagg cgcatctcta
      901 tccgaacccc gaagtcttcg atcccactcg cttcgcgccc gagcaggtgg cgacgcgcca
      961 tcgcttcgcg tatctgccgt ttgccgccgg tcatcgcaac tgcatcggcg ccggccaggc
     1021 gctggtggag ctcaagctca tcgtgtcgcg catcgcccag cgcttcgaac tggacctcgc
     1081 gccgggccac aaggtggaac cggctcccgg caccacgatg ttcccgcgtt acggcatgaa
     1141 gatgaagatc cggcacatca aggccgcgcg atgagcgttc cggcggattc ccgcagcgaa
     1201 caggcgcgcc agttctacaa cgcggctgcg agcacctcga ccgatttcgc gttcatgaat
     1261 tacggatacg cgccgttggc ctccgatctc gacggcactg cggagcctga aaaatattgc
     1321 ctgcaactct accggcgcct gctcggcaac gtggatctgc gcggcaagaa ggtggtggaa
     1381 gtgagctgcg gccgcggtgg cggtgcggca cacgtcgccg ccacgtacca gcctgcgagt
     1441 tacctcggca tcgacatcag cgaacggaat ctgagtcttg cgatgcaccg gttccccggt
     1501 gtttcttcgc tgagtttcca ggtgggtaac gccgaagcgc tgccgctgcc ggatgcgagc
     1561 ttcgacgcgc tgctcaatgt ggaagcctca cacctctatg acaacccggc gcggttcttc
     1621 gccgagacgt ttcgcgtcct gaagccgggc ggcacgtttt tccacgtgga cctcgcctgg
     1681 aaggacaagg atccgccggc catgatcgcg gccgcgggat tcaagattgc gtcggtggaa
     1741 gacatcacga aaaacgtcgt gctggcgctg gacctcgaca gcgtgcggcg cgaagccatc
     1801 gtcatgagtt tccccgagaa catgcgcaac gacttccgcg actggtctgg cgtgaagggt
     1861 ttccgcgcgt acaaccgcct gcagaatggc gagtgggtct accggatcac gcgcggcaag
     1921 cggtaatggg gacactcccc atttgctagc aaaaggggcc gcacctcccc gatcgcggac
     1981 gcaaacgcgt tccgtgtaac gatttctcgt gaccc
//
LOCUS       DATDEU010000250         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1503509, whole genome shotgun sequence.
ACCESSION   DATDEU010000250 DATDEU010000000
VERSION     DATDEU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503509"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VIV63_06115"
     CDS             complement(<1..579)
                     /locus_tag="VIV63_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY6124205.1"
                     /translation="MTKSSSLATAPQNLSDRVAHHLMDHIRTNRLASGERLPSEVRLS
                     ADLKISRGIVREAYRALRSAGILEISNGRSPRVGGLRSTSLIHLLQHALATQQASPEQ
                     ALDLRSAIEVRAAELAAEKRSDKDIEVLTRSVRGMKRARRNIDDFVKHDVRFHECLGT
                     ATGNPLFSLISSALSEAMAISVRAGMESRSDQA"
     gene            complement(599..1630)
                     /locus_tag="VIV63_06120"
     CDS             complement(599..1630)
                     /locus_tag="VIV63_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY6124206.1"
                     /translation="MAASTVTFGEIMLRLTPPGFERLLQSPTMHASFGGGEANVAASL
                     ASFGLPATFVTILPQANPLADAAIAELRRFGVDTSQIARSAGRMGIYFLETGASQRAS
                     RVVYDRAGSAFALASTSIDWPRVLAGAGWLHVTGITPAVSEAAANLAMEAARTARGMG
                     VTVSCDLNFRKNLWKWGKTASEVMPELVKLSDILIANEEDIQMALGLASDVDVHAGTL
                     DRSSFCGLTEAVMREYGHLRGIAVTLRESKSASHNVWSACLHDGREFLLSRTYDIANI
                     VDRVGAGDSFAAGLIYGMQSGLGSQSALDFAVAASCLKHSIPGDINRVSVDEVKALLD
                     GGGSGRVQR"
     gene            complement(1630..2280)
                     /locus_tag="VIV63_06125"
     CDS             complement(1630..2280)
                     /locus_tag="VIV63_06125"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005119.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEY6124207.1"
                     /translation="MALVRLDILNKIVGPGLVAVIRASSAEQAARIADACVKGGIQAL
                     EVTYTVPNATAVIAELSKRYASQPVAIGAGTVLDPETARAAILAGAQFIVSPGSDAAT
                     ARLCRRYQAPYLPGAATPTEIIRALEDGADIVKVFPGEVLGPAFVKAVRGPLPQAPLM
                     PTGGVSAENAAEWIRSGCVALGVGGELTKGAADDEAISERARLLLEAIRKARATVA"
BASE COUNT          480 a          875 c          845 g          461 t
ORIGIN      
        1 ggcttggtcg ctgcggctct ccatgcctgc gcgtaccgag atcgccattg cctcgctgag
       61 cgcgctcgaa atcagcgaaa acaacggatt gccggtcgcg gtgccgaggc actcgtgaaa
      121 gcgcacgtcg tgcttgacga agtcgtcgat gttgcggcgc gcgcgcttca tcccccgcac
      181 tgaacgggtc aggacctcga tgtccttgtc gctgcgtttt tcggcagcga gctccgccgc
      241 gcgcacctcg atcgccgagc gcagatccag ggcctgctcg ggtgacgcct gctgcgtcgc
      301 cagcgcgtgc tgcagcagat ggatcaatga agtgctgcgc aggccaccaa cacgcggcga
      361 tcgcccgttg gaaatctcga ggatccccgc gctgcgcaac gcccgatacg cttctcgcac
      421 gatgccgcga ctgatcttca agtcggcgct caggcgcacc tcggacggca agcgttcgcc
      481 ggaagcgaga cggttggtgc ggatgtgatc catcaggtgg tgagccaccc gatcggacag
      541 attctgcggt gcggtcgcga gcgacgacga tttggtcatc gaaggttaaa gctgtcgatc
      601 aacgttgcac gcgcccggat ccgcctccgt ccaacagcgc tttgacttca tcaacactga
      661 cgcgattgat atcgccgggg atcgaatgct tcaggcagct ggcggcgacg gcaaaatcga
      721 gcgcactctg tgaccccagg ccgctttgca tcccatagat gagtcccgcg gcaaagctgt
      781 cgccggcgcc gacccgatcg acgatgttcg caatgtcgta agtgcggctg aggaggaact
      841 cgcggccgtc atgcaggcac gccgaccaga cgttgtgcga cgcgctcttc gactcgcgca
      901 gcgtcaccgc gatcccccgc aggtgaccgt actcgcgcat cacggcttcg gtcaggccgc
      961 aaaagctcga gcgatccagc gtacccgcgt gcacgtcgac gtccgatgcc aggccgagcg
     1021 ccatctgaat gtcttcttcg ttcgcgatga ggatgtccga gagcttcacg agctccggca
     1081 ttacctcgga cgcggtcttt ccccatttcc agaggttctt ccgaaaattc agatcgcacg
     1141 agacggtgac gcccatgccg cgcgctgtac gcgcggcctc catcgcaagg ttcgccgccg
     1201 cctcgctcac cgcgggcgtt atcccggtga cgtggagcca tccggcgccg gcaaggacgc
     1261 gaggccagtc gatgctggtg gacgcgagag cgaaggcact gcccgcgcgg tcgtagacga
     1321 cccgtgatgc cctctgactt gcgcccgttt ccaggaagta gatccccatg cggcctgccg
     1381 accgcgcaat ctgtgacgtg tcgacgccaa agcggcggag ctcggcgatc gccgcgtccg
     1441 ccagaggatt cgcctgcggc aggatggtca caaacgtcgc cggcagaccg aagctggcga
     1501 gcgacgccgc aacgttggct tcgccgccgc cgaaggacgc gtgcatcgtg ggactctgca
     1561 gaagacgctc gaatcccgga ggcgtcaacc ggagcatgat ctcgccaaac gtgacggtcg
     1621 aagcggccat tatgctaccg tcgctcgcgc tttccggatc gcctcgagca gcaggcgcgc
     1681 cctttccgag atcgcctcgt cgtctgctgc gcccttggtc agctcgccgc ccacaccgag
     1741 cgcgacacac cccgagcgga tccactccgc ggcgttctct gctgagactc cgccggtcgg
     1801 catcagcggt gcctgcggca gcggcccgcg cacggccttg acgaacgcag gcccaagcac
     1861 ttcaccagga aagaccttga cgatgtcggc accatcttcg agggcgcgga tgatctcggt
     1921 cggcgttgcc gcgcccggaa ggtacggcgc ctggtagcgg cgacaaagcc gcgcggtcgc
     1981 ggcatccgag ccgggactga cgatgaactg cgcgccggcg agaatcgcgg cgcgggcggt
     2041 ctcgggatcg agcaccgtgc ccgcaccgat tgcgaccggc tgcgaggcgt agcgcttcga
     2101 caactcggca atcaccgccg tggcattcgg cacggtgtag gtcacttcta atgcctgaat
     2161 gccgcccttg acgcaggcgt ccgcgatgcg cgccgcctgt tcggcgctcg acgcgcgaat
     2221 caccgccacc agccccggcc ctacgatttt gttgaggatg tccagtctga ccaaagccaa
     2281 tccggcgcag tatgtcacct ggtcacgacc tattcaatag gttcgagtat tgacacctct
     2341 gaccggaccc gtgtgaaccg tcgtcgtaaa aggggccgga aggaatctgg gcatagcgag
     2401 actccttccg gcgcaaacgc atgatgcctt cgccgcccgc ttccgccttc ggcgggagcg
     2461 ttgggaagaa cgcgatgctg aaggaggaat cggtgagctt acagcggctg gaactgctgc
     2521 tgcgagaact gctgcgagag gcagaacgac aggccagtcg tgcggctggg gaacagcgtt
     2581 tcgatgaatg cgcccgtctt catgcttgac gtagaagcta tcagccgcgc tggtgcgaat
     2641 gcaatagatg acgcacttgc a
//
LOCUS       DATDEU010000251         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_628292, whole genome shotgun sequence.
ACCESSION   DATDEU010000251 DATDEU010000000
VERSION     DATDEU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628292"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="VIV63_06130"
     CDS             complement(<1..1491)
                     /locus_tag="VIV63_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HEY6124208.1"
                     /translation="MQSSSNCWHCGEALSGAVIHARVAGEQRPMCCQGCRAAAEWIEQ
                     LGLAGYYDLRTAPAQKPGPDANPAHDSWDEESSRHVIRDVGNGLRESLLLVEGVRCTA
                     CVWLIERSLGTMPGVVRVQVNAVAQRARVIWRASVISLPQLLRALARIGHRAQPLDAR
                     GLDDLRRRESRDALKRLLVAGFGAMQAMMYATAIYLGAADALDAPTRDLLRWLGLLVA
                     TPVVLYSARPFYTGAWRSLKAHRVGMDVPVALAICAVYLASVIEAVRGSGEVYFDSVS
                     MFVFFLLAGRYLEMRARHRARDLTDALARLTPRCADRRRVDGTLQRVGIHELRAGDCV
                     RVAEGGIVPADGVLQSERCRVDEALLSGESSPVTKRRGEMLIAGSVLEDGPVFVRVER
                     VGADTALAGIAALVGRAHTLRPRLQVAGERAAGRFVARILALTALTSVAWSFIDPTRA
                     FPAAIAVLVISCPCAFALAIPAALTRALGAMARCGVLVVKPDAVQSL"
     gene            complement(1614..>2024)
                     /locus_tag="VIV63_06135"
     CDS             complement(1614..>2024)
                     /locus_tag="VIV63_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6124209.1"
                     /translation="TILTSILDGRNGVMPPLAGALGHNGVNEVAAYVVSAAGTQVPPG
                     WAAAGKTRFDALCSACHGVDGRGNPALGAPNLFDNAWLYGSSIESVVASVRDGRSGVM
                     PAWRQRLSTDEARMITAWIVAQGMREGMREGRRE"
BASE COUNT          351 a          715 c          691 g          267 t
ORIGIN      
        1 gagtgactgg acagcatccg gcttcacgac gagcacgcca caccgggcca tcgcgcccag
       61 cgcgcgcgtg agcgcggcgg gaatggcgag cgcaaacgcg caggggcagg aaatcacgag
      121 caccgcaatc gcggcgggga aagcacgcgt gggatcgatg aaactccagg cgacggacgt
      181 aagggcggtc agcgccagta tccgcgcgac gaagcggccg gcagcgcgct cgccggcaac
      241 ctgcagccgg ggacgcagtg tgtgcgcgcg gccgacgagc gcggcgatgc cggccagcgc
      301 cgtgtcggcg cccacgcgct cgacgcgcac gaacacgggc ccatcctcga gcacgctgcc
      361 ggcgatcagc atctcgccgc ggcgcttggt cacgggagac gactcaccgc tcagcagtgc
      421 ttcatcgacc cggcagcgtt cgctctgcag cacgccatcg gcaggcacga tgcctccctc
      481 ggccacgcgc acgcagtcgc cggcgcgcag ctcgtggatg cccacgcgtt gcagcgttcc
      541 gtcgacccgt cttcgatccg cgcatcgcgg cgtcagacgc gccagcgcat cggtcagatc
      601 tcgcgcgcga tgccgcgcgc gcatttccag gtaacgaccc gcgagcagga agaacacgaa
      661 catcgacacc gaatcgaagt acacctcacc gctgccgcgc accgcttcga tgacgctcgc
      721 caagtacacc gcgcagatcg ccagtgcgac cggcacgtcc attcccacgc ggtgcgcctt
      781 gagagagcgc caggcgccgg tgtagaacgg ccgcgccgag tagagaacca ccggtgtggc
      841 caccagcaat ccgagccagc gcagcagatc gcgcgtgggt gcatcgagtg catcggcagc
      901 gcccaggtag atggcggtgg catacatcat ggcctgcatg gcgccgaaac cggcgaccag
      961 caatcgcttg agcgcatcgc gcgactcgcg gcgacgcagg tcatcgaggc cgcgagcatc
     1021 gagtggctgg gcgcgatggc cgatgcgcgc gagagcccgc agcagctgcg gcagcgagat
     1081 gaccgaggcg cgccagatga cgcgtgcgcg ttgcgcgacc gcattcacct gcacgcgcac
     1141 cacgcccggc atcgtgccga gcgaccgttc gatcagccag acgcaggcgg tgcaacgcac
     1201 gccttcgacc agcaggaggg attcgcgcag gccattaccc acatcgcgaa tgacgtggcg
     1261 ggagctttcc tcgtcccatg agtcgtgcgc cgggttcgca tcagggcccg gcttttgcgc
     1321 gggcgccgtc cgcaagtcgt agtagccggc aaggccgagc tgctcgatcc attccgccgc
     1381 ggcacggcag ccctggcagc acatcgggcg ctgctcgccc gcgactcgcg catgaatgac
     1441 ggcgcccgaa agcgcctctc cgcaatgcca gcagttagag gagctctgca cgggcgcggc
     1501 tcatgcggag ttcgccgcga agatcagggc gacgcagccg gccagcgagc cgagcaggat
     1561 gacgacgccc agccacatca cgggcggtcg gtaccagggt tcgccatcgc ggttcattcg
     1621 cgcctgccct cgcgcattcc ctcgcgcatt ccctgggcga cgatccaggc cgtgatcatg
     1681 cgcgcctcgt cggtcgaaag gcgctggcgc cacgcgggca tgacgccact gcgcccatca
     1741 cgcacgctgg ccacgacgct ttcgatgctg ctcccgtaca gccaggcgtt gtcgaagaga
     1801 ttgggtgcgc cgagggccgg gtttccgcgg ccgtcgacac cgtgacaggc ggagcacagc
     1861 gcgtcgaacc gcgttttgcc tgccgccgcc cagccgggcg gcacctgcgt gccggccgcg
     1921 ctaactacat aggcggcgac ttcgttcacg ccgttgtggc cgagcgcgcc cgcgagcggg
     1981 ggcatgacgc cgttgcggcc atccaggatg ctggtgagga tggt
//
LOCUS       DATDEU010000252         3834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1649103, whole genome shotgun sequence.
ACCESSION   DATDEU010000252 DATDEU010000000
VERSION     DATDEU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649103"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2309
                     /gene="dnaE"
                     /locus_tag="VIV63_06140"
     CDS             <1..2309
                     /gene="dnaE"
                     /locus_tag="VIV63_06140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY6124210.1"
                     /translation="RVIEYVAQKYGKERVSQIITYGTMAAKAVVRDVGRVLGMGYGYV
                     DKIAKLIPFELGITLDDALEKEPELKRLYKEDEEIKNLMDLARSLEGLTRNAGMHAGG
                     VVIAPSKLTEFAPLYADDKGGSVVTQFDKDDVEAAGLVKFDFLGLRTLTVIDRAVKII
                     NARRAAAGEALLDISQLPMDDQATYDLMAEAKTTAVFQLESRGMKDLIRRLKPDRFED
                     IVALVALFRPGPLESGMVGDFIDRKHSRNDPHAPPIDYLHPSLQPVLAPTYGVILYQE
                     QVMQIAQVLASYTLGGADLLRRAMGKKKPEEMAKERGKFVSGSVKNGVPESQAAHIFD
                     LMEKFAGYGFNKSHSAAYALLSYQTGWLKTHEAASYMAAVLSADMDHTDKVVGMLYDC
                     RKEVGLTVLPPHVNLSSYEFAVVDDKTIRYGLGAIKGVGQGAVESIVSERKANGDYGS
                     LEDLCRRLDLSKINRRVLEALIKSGSLDGLAANRATLMHRLDAALALGEQNSKANETG
                     QHDIFGLSAAHVPEAVRASAPDQPEWTDSVRLRSERETLGRAISGHPIDRFMNDLPRF
                     ITGRIVDYIDAEKPQASAEGGRSFFGGKPAAIAGVVEELRKRGPRTSVVLDDDSGRIE
                     CSFFDEQYQQYREILLKDALLLIEGKVRFDEFANTWVLRASKVSELERLREKEARRIV
                     LKLKSTDKLPLEKLQAILAQYRGGACQVSVQYYAAGARGTFSFGAEWNVRPAPALTEE
                     LEKLLGRGRIAVLYSPPPVSAGVTSING"
     gene            2327..3292
                     /locus_tag="VIV63_06145"
     CDS             2327..3292
                     /locus_tag="VIV63_06145"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY6124211.1"
                     /translation="MAVNFLDFEQPIAELEAKIEELKHVPSGSAVNIQEEITRLQAKS
                     EQLTQSIFANLTPWQITQLARHPQRPYTLDYAGLIFKDFQELHGDRMYGDDLAIIGGP
                     ARLGDTPVMLIGHQKGRDTKERVRRNYGMPKPEGYRKALRLMRLAERFRLPLITFIDT
                     PGAYPGVGSEERNQSEAIARNLFEMSVMGIPIISCVIGEGGSGGALAIGVCDRLLMLQ
                     FSTYSVISPEGCASILWKSADKKEVAADAMNLTADRLFKLKLVDEVLPEPVGGAHRDA
                     TAVANTIKTALVKHLSQLEKLPPDKLRAQRNTRIDGFGVFTEVAS"
     gene            3335..>3834
                     /gene="tilS"
                     /locus_tag="VIV63_06150"
     CDS             3335..>3834
                     /gene="tilS"
                     /locus_tag="VIV63_06150"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY6124212.1"
                     /translation="MIDPVAKLLELAGPKPRVALAISGGIDSTVLAHLLVKQRRKFGS
                     VRLLHVDHGLQAASGEWSRHCQQLARQWKVPIVCLKASIVRGKDSPEAAARDARYSLL
                     AGAMEPGVVLVTAQHRDDQAETLLLQLLRGAGVPGLAAMPPFAPFGPGFIARPLLAVT
                     RAELEAV"
BASE COUNT          734 a         1241 c         1225 g          634 t
ORIGIN      
        1 accgcgtcat cgaatacgtg gcgcagaagt acggcaagga acgcgtctcg cagatcatca
       61 cctacggcac catggccgcg aaggccgtgg tgcgcgacgt gggccgcgtg ctcggcatgg
      121 gctacggcta cgtcgacaag atcgccaagc tcattccctt cgagctcggc atcacgctcg
      181 atgacgcgct cgaaaaggag cccgagctca agcgcctcta caaggaagac gaggagatca
      241 agaacctcat ggacctcgcg cgctcgctcg aaggtcttac gcgcaacgcc ggcatgcatg
      301 ccggtggcgt ggtcattgcg ccttcgaagc tcacggagtt cgcgccgctg tacgccgacg
      361 acaagggcgg cagcgtcgtc acgcagttcg acaaggatga cgtcgaagcc gcgggcctcg
      421 tgaagttcga cttcctgggg ctgcgtacgc tcacggtcat cgaccgtgcc gtgaagatca
      481 tcaatgcgcg gcgcgcggcg gcgggcgaag cgctgctcga catcagccag ttgccgatgg
      541 acgaccaggc cacctacgac ctcatggcgg aggcgaagac cacggccgtg ttccagctcg
      601 aatcccgcgg catgaaagac ctgatccggc gcctgaagcc ggaccggttc gaagacatcg
      661 tggcgctggt ggcgctcttc cgcccgggtc cgctcgaatc gggcatggtc ggcgacttca
      721 tcgatcgcaa gcacagccgc aacgatccgc atgcgccgcc gatcgactat ctacatccgt
      781 cgctgcagcc cgtgctcgca ccgacctatg gcgtgatcct gtaccaggaa caggtgatgc
      841 agatcgcgca ggtgctcgcg agctacacgc tcggcggcgc cgacctgctg cgccgcgcga
      901 tgggcaagaa gaagcccgaa gaaatggcga aggagcgcgg caaattcgtc agtggttcgg
      961 tcaagaacgg tgtgcccgag tcgcaggccg cgcacatctt cgacctcatg gagaaattcg
     1021 ccggttacgg cttcaacaag tcgcactcgg cggcgtacgc gttgttgtcg taccagaccg
     1081 gctggttgaa gacgcacgaa gccgcgtcgt acatggcggc ggtgttgtcg gccgacatgg
     1141 accacaccga caaggtggtc ggcatgttgt acgactgccg caaggaagtg ggactcaccg
     1201 tgctgccgcc gcacgtgaat ctgtcttcgt acgaattcgc ggtggtcgac gacaagacca
     1261 ttcgctacgg cctcggcgcc atcaagggcg tggggcaggg cgcggtcgag tcgatcgtca
     1321 gcgagcgcaa ggcgaacggc gactacggca gtctcgaaga tctttgccgc cggctcgacc
     1381 tgtcgaagat caaccggcga gtgctcgagg cgttgatcaa gtccggaagt ctcgacggac
     1441 tcgcggcaaa tcgagccaca ctcatgcatc ggctcgatgc ggcgctggcg ctcggcgagc
     1501 agaacagcaa ggccaacgag accgggcagc acgacatctt cggcctgagc gccgcgcatg
     1561 tgcccgaagc cgtgcgcgcg tccgcgcccg accagccgga gtggaccgac agtgtgcggc
     1621 tgcgctccga gcgagagacg ttgggacgcg ccatctcggg ccatcccatc gaccgcttca
     1681 tgaacgatct gccgcggttc atcaccgggc gcatcgtcga ctacatcgac gctgaaaagc
     1741 cccaggcgag cgccgagggt ggccgctcct tctttggcgg caagccggcg gcgattgccg
     1801 gcgtggtcga ggaattgcgc aagcgcggac cccggacctc agtggtgctc gacgacgact
     1861 cgggccgcat cgaatgcagt ttcttcgacg agcagtatca gcagtaccgc gagatcctcc
     1921 tgaaggacgc gctgctgctc atcgagggca aggtgcgatt cgacgagttc gcgaacacct
     1981 gggtgctgcg tgccagcaaa gtcagcgagc tcgaacgtct tcgagagaag gaagcacgcc
     2041 gcatcgtgct caagttgaaa tcgaccgaca aattgccgct cgaaaagctt caggccatcc
     2101 tggcgcaata ccggggtggc gcctgccagg tgtccgttca gtactacgct gcgggggcac
     2161 gcggcacgtt ctcgttcggt gccgagtgga acgtccgtcc cgcgcctgcg ctgaccgagg
     2221 agctcgagaa gttgttgggt cgcggccgga tcgcggtgct gtacagcccg ccgccggtga
     2281 gcgccggagt cacctccatc aacggatagg gtattctcgc gcccctatgg cagtgaattt
     2341 cctcgatttc gagcagccga tcgccgaact tgaagccaag atcgaagaac tgaaacacgt
     2401 tccttcgggc tccgcggtca acatccagga agagatcacc cggctgcagg ccaagagtga
     2461 gcagctcacc cagagcatct tcgcgaacct gactccgtgg cagatcaccc agctcgcgcg
     2521 tcatccgcag cggccctata cgctcgatta cgccgggctc atcttcaagg acttccagga
     2581 gctgcatggc gatcgcatgt atggcgacga tctcgccatc atcggcggcc ccgcgcggct
     2641 cggcgacacg cccgtcatgc tcatcggcca ccaaaagggc cgcgacacga aagagcgcgt
     2701 gcgccgcaac tacggcatgc cgaaaccgga aggttatcga aaggcgctgc gcctcatgcg
     2761 cctcgcggaa agattccggc tcccgctcat cacgttcatc gacacgccgg gcgcgtatcc
     2821 gggcgtgggt tcggaagaac gcaatcagtc cgaagccatc gcgcgcaatc tgttcgagat
     2881 gtccgtcatg ggcattccca tcatcagctg cgtgatcggt gagggtggat cgggcggcgc
     2941 gctggccatc ggcgtctgcg accggttgct gatgctgcag ttctccacct actcggtgat
     3001 ctcgccggaa ggctgcgcgt ccattctctg gaagagcgca gacaagaaag aagtggcggc
     3061 ggacgccatg aacctcacgg ccgatcgcct gttcaagctc aagctcgtcg acgaagtgtt
     3121 gccggagccc gtgggcggcg cgcatcgcga tgccaccgcg gtcgccaaca ccatcaagac
     3181 cgcgctcgtg aaacatctct cgcagctcga gaaactgccg cccgacaagc tgcgtgcgca
     3241 gcgtaatacc cgcatcgacg gtttcggcgt tttcaccgaa gtcgcgagtt aatggggaca
     3301 ctccccattt gctagcaaaa ggggccgcac ctcaatgatc gatccggtcg cgaagctcct
     3361 cgagctcgcg ggaccgaagc cgcgcgtggc gctcgccatc agcggcggca tcgactccac
     3421 ggtgctcgcc catctgctgg tgaagcagcg gcgaaaattc ggcagtgttc gcctgctgca
     3481 tgtcgaccac ggactccagg ccgcgagcgg cgagtggagc cggcactgcc agcaactcgc
     3541 gcgccaatgg aaggtgccca tcgtctgcct gaaggccagc atcgtgcgcg gcaaggactc
     3601 tccggaagcc gccgcgcgtg acgcccgtta ctcgctgctg gcgggcgcca tggagcccgg
     3661 cgtagtgctg gtcaccgccc aacatcgcga cgaccaggcg gagacgctgc tgttgcagct
     3721 gctgcgtggc gccggcgtcc ccggcctcgc ggcgatgccg ccattcgcgc cattcggccc
     3781 tggcttcatc gcgcgcccat tgcttgcggt cactcgcgcc gaactcgaag ccgt
//
LOCUS       DATDEU010000253         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1649811, whole genome shotgun sequence.
ACCESSION   DATDEU010000253 DATDEU010000000
VERSION     DATDEU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649811"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VIV63_06155"
     CDS             <1..784
                     /locus_tag="VIV63_06155"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY6124213.1"
                     /translation="YTWYEFCDWYLELTKPVLQGENSTEAQKRGTRRTLVTVLEALLR
                     ALHPMMPFITEEIWQRVHPLAAPLLSAQNSRANQKSVDSVMAAAYPAASDYARDTDAE
                     TQVTLLKEYILKVRQIRGEMNIAPSRKIPLLVRDADEQAQILIRNNAHYLHRLAGLES
                     LKVMNAGDAEPVSASAMVAGTTLLVPLAGLIDVAAEIDRLTKVIAKNESDIGKLRGKL
                     GNESFVKNAKPELVEADRAKLAELIAQNESMGKQLERLRRLG"
     gene            837..1778
                     /locus_tag="VIV63_06160"
     CDS             837..1778
                     /locus_tag="VIV63_06160"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6124214.1"
                     /translation="MNPPSTTVNPALTAALAALDRVILGKPEAVRLALTCLLARGHLL
                     IEDVPGVGKTTLAHALAQVLGLAWQRVQFTSDLLPADIIGVSVFDAASGGFSFRKGPM
                     FTQLLLADEVNRASPKTQSALLEAMEERQVSVDVTTYRLPDPFFVVATQNPFEQLGTF
                     RLPESQLDRFLMRISLGYPDAANERALLAEGDRRDLLSGMTPALNPDGVRELQAAAQR
                     VHVAPALLEYVQTLVASTRQRTDVLLGLSPRAGQGLVRAARAWAMLAGRDMVIPEDVQ
                     AVFAPVATHRLERAGVATAIDHAAFIAELKRGVPVPV"
     gene            1796..>2158
                     /locus_tag="VIV63_06165"
     CDS             1796..>2158
                     /locus_tag="VIV63_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124215.1"
                     /translation="MREVSGTLKENLRRRMGAWARRRQGDDRLPIVVNSRRLYILPTR
                     AGLAFAALLLVMMLAGLNYSNSIALLITFLLAGFGLIAMHLTHRNLVGVTLRAVASVD
                     AFVGEHGRLLVTLDNSADT"
BASE COUNT          377 a          776 c          669 g          336 t
ORIGIN      
        1 atacacctgg tacgagttct gcgactggta cctggaactg acgaaaccgg tgctccaggg
       61 tgagaactca accgaagcgc agaagcgcgg cacgcgccgc acgctggtga cggtgctcga
      121 ggcgctgcta cgcgcgctgc atcccatgat gccgttcatc accgaagaaa tctggcagcg
      181 cgtgcatccg ctggcggcgc cgctgctctc ggcgcagaac tcgcgcgcca accagaaatc
      241 cgtggacagc gtgatggccg cggcctaccc ggccgccagc gattacgcgc gcgacacgga
      301 cgccgaaacg caggtcaccc tgctcaagga atacatcctg aaggtccggc agatccgcgg
      361 cgagatgaac attgcgccct cgcgcaagat tccgctgctg gtccgtgacg ccgacgaaca
      421 ggcgcagatc ctcatccgca acaacgcgca ctatctacac cgcctggccg gcctcgagag
      481 cctcaaggtc atgaacgcgg gtgatgcaga acccgtgtcg gccagtgcca tggtggcagg
      541 caccacgctg ctggtgccgc tggcgggact catcgatgtc gcggcggaga tcgaccggct
      601 caccaaggtc atcgcgaaaa atgaatccga catcggcaag ctgcgcggca agctcggcaa
      661 cgagagtttc gtgaagaacg ccaagccgga actcgtcgaa gccgaccggg ccaaactcgc
      721 cgaactcatc gcccagaacg aaagcatggg caaacagctc gaaaggctcc ggcgcctcgg
      781 ctgaagcttg cgcgccatca cgaatattcg accgcggcga aggatatagt cgggtcgtga
      841 acccaccgag caccacggtc aaccccgccc tcaccgccgc cctggccgcg ctggatcgcg
      901 tcatcctcgg caaacccgag gcggttcggc tggccctcac ctgcctgctg gcccggggtc
      961 acctgctcat cgaagacgtg cccggcgtgg gcaagaccac gctcgcgcat gcgctcgcgc
     1021 aggtactcgg cctcgcctgg caacgcgtgc agttcaccag cgacctcttg cccgcggaca
     1081 tcatcggcgt gtcggtcttc gatgccgcca gcggcggctt ctcgttccgc aagggcccga
     1141 tgttcactca gctcctgctg gcggacgaag tgaaccgcgc cagcccgaag acacagagcg
     1201 cgctgctcga agccatggaa gaacggcagg tcagcgtcga cgtgactacc taccgcctgc
     1261 ccgatccgtt cttcgtggtc gccacgcaga atcccttcga gcagctcggc acattccgcc
     1321 tgccggaatc gcagctcgac cggttcctca tgcgcatttc gctcggctac ccggacgcgg
     1381 ccaacgagcg cgcactgctg gccgaaggcg accgccgcga tctgctctcc ggcatgacgc
     1441 cggcgctcaa ccccgacggt gtgcgcgagc tgcaggcggc ggcgcaacgc gtgcacgtcg
     1501 cgcccgccct gctcgaatac gtgcagacgc tggtcgcgag cacccgccag cgcaccgacg
     1561 tgttgttagg cctgagcccg cgcgccggcc agggcctggt gcgcgccgcg cgtgcctggg
     1621 ccatgctcgc cggccgcgac atggtgatcc ccgaagacgt ccaggccgta ttcgcgcccg
     1681 tggccacgca ccgtctcgaa cgcgccggtg tcgccacggc gatcgatcac gccgccttca
     1741 tcgccgagct caagcgcggc gttccggttc cggtgtagcg ctcatgggcg ccgcggtgcg
     1801 cgaagtatcc ggcaccctca aggaaaatct gcgtcgccgc atgggcgcct gggcgcgccg
     1861 tcgccagggt gacgaccggc tgcccatcgt cgtcaactcg cgccgcctgt acatcctccc
     1921 cacgcgcgcc gggctggcat tcgccgcgct gttgctggtc atgatgctcg ccgggctcaa
     1981 ctactcgaac agcatcgcgt tgctcatcac cttcctgctc gccggcttcg gcttgatcgc
     2041 gatgcatctc acgcaccgga acctcgtggg cgtcaccttg cgcgccgtgg cgagtgtcga
     2101 tgcgttcgtc ggcgaacacg gccggctgct ggtcacgctc gacaactccg ccgatacc
//
LOCUS       DATDEU010000254         3123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1649934, whole genome shotgun sequence.
ACCESSION   DATDEU010000254 DATDEU010000000
VERSION     DATDEU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649934"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..745
                     /locus_tag="VIV63_06170"
     CDS             272..745
                     /locus_tag="VIV63_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124216.1"
                     /translation="MRTAFVSLLCLVASVAAASNYREVARAVNDDQMLQTAGVTPAEA
                     QIRQQCSAAIIETDDRPEFFDCVYVQTDKDLNLFSLEDGYLMSELQLKLHNMDGVALQ
                     RMGRYSQVQIFSRDQVTVFYIYGKSWIDPVQTENVYQWLTNHGVPARQPRKWIGP"
     gene            1013..2107
                     /locus_tag="VIV63_06175"
     CDS             1013..2107
                     /locus_tag="VIV63_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6124217.1"
                     /translation="MSLHKLMPEKTGDASVNVRVSMNHTINDIGEAIVHSRAIQAFDH
                     AAKSFPIHRMVRCAVRRKVADVFDDLALQMNCAAQRIDEGTLLLDAPGMFVFAEGHTK
                     GEYTSCSIRLWTDSIARCDEVRQRLLRVVGDQRMRDQMFVVDWNFCNSSGYLVSTSFD
                     EIADEVLLDEAYPSLGKSVAEFVQGYLDARETVLILQGPPGTGKTRLVRAVLAAMSRR
                     KGDSAKVMFTADKRAIENDEIFVDFITGSHDAFVIEDADHLLKARTSGNFELHRFLSV
                     ADGVVRAQNRKVIFTTNLPNINDIDDALLRPGRCFAVKNLRSMDLAEADRLVERLCGG
                     DAARLARARSSLESVGGKSRSVAQVYRACA"
     gene            complement(2120..2542)
                     /locus_tag="VIV63_06180"
     CDS             complement(2120..2542)
                     /locus_tag="VIV63_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124218.1"
                     /translation="MIREGRKFGFALGLLAPCLLAVPCIATAGDDAARQSNKTAAPLE
                     IPHTTNNELDARAKKKRAPGNLEKTALLDLDPALTPLPFPKNSDMRARLLTPELRRTP
                     LLGWIAENLYRSKSEKGWCLEADPGQGEYVVFYRLHLK"
     gene            2757..>3123
                     /locus_tag="VIV63_06185"
     CDS             2757..>3123
                     /locus_tag="VIV63_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HEY6124219.1"
                     /translation="MTIDERINATLAPIADWLGKVVFFAVPVGEAQLPLIVAWLIMGG
                     VVCTIAFRFVNLRGFGHSARVIRGDYSSKSHAGEATPFQALSTAVSGTVGLGSSGGVA
                     VAVTLGGPGATRWMMLAGFL"
BASE COUNT          545 a          963 c         1008 g          607 t
ORIGIN      
        1 agattgcttt atctagatcg tctgcggaat gttctcctcc cccctcgtga gactttttgc
       61 tggacagacg cgtacaggaa cggaagggcc tgtacgcgtt tttttttgcc caaaggaaat
      121 ggggcatgga agtgttggga ccgacttaag tctgtcccca ctcgtttagc tagcaaatgg
      181 ggacgcggct cagttcgcgt cggcacaaat ccgctccggt cgtaatgctc actgacaggg
      241 ccgccgcgag ccgaagaata aagtcggccc catgcgtacc gctttcgttt cattgctctg
      301 cctcgtcgca agcgtggccg ccgcctcgaa ctatcgcgag gtggccaggg ccgtgaacga
      361 cgaccagatg ctccagaccg cgggtgtcac cccggccgaa gcgcagatcc gccagcagtg
      421 ttctgccgcg atcatcgaga ccgacgatcg gcccgagttt ttcgactgcg tttatgtcca
      481 gacggataag gacctcaacc tcttcagtct cgaagatggg tacctgatgt ccgagctgca
      541 gctcaagctg cacaacatgg atggcgtggc gctgcagcgc atgggccgat actcccaggt
      601 gcagatcttc agccgcgacc aggtgaccgt gttctacata tatggcaaat cgtggatcga
      661 tccggtgcag accgagaacg tgtaccagtg gctcaccaat cacggtgtgc cggcgcgcca
      721 gccgcgcaag tggatcggcc cctagcaatt tcttgacgcg gggcttgaca cccgcgttat
      781 cgcgcctaga atgcgcgccc tcatgaaacc taactacctt agtttccagt tcacgcatgc
      841 gttcgcggcc ccgccgcgcg acttcgtgcg cgaacgcaat atttgctagc ccttcattta
      901 cggagggcgg cattcggctg ctctccggca agtctcttac gaggccccgg tcggctgccg
      961 ccaccggggc ctcgtcattt ggggcgaggc cccagcgcca ggaacaagag tcatgtcact
     1021 gcacaagttg atgcccgaga agaccggcga cgcgtcggta aacgtacgcg tctcgatgaa
     1081 ccacacgatc aacgacatcg gtgaagccat cgtgcattcg cgcgcgatcc aggctttcga
     1141 tcatgccgcg aagtcctttc cgatccatcg catggtgcgt tgtgccgtgc gccgcaaggt
     1201 cgccgacgtg ttcgacgatc tcgcgctgca gatgaactgt gccgcgcagc gcatcgacga
     1261 aggcacgttg ctcctcgacg ctccgggcat gttcgtattc gccgagggtc acaccaaggg
     1321 tgagtacacc tcgtgctcga tccgcctgtg gacggattcc atcgcgcgtt gcgacgaagt
     1381 gcgccagcgc ctgctgcgcg tggtcggcga tcagcgcatg cgcgaccaga tgttcgtggt
     1441 ggactggaac ttctgcaatt cgagcggtta tctcgtgagc acgtcgttcg acgaaatcgc
     1501 cgacgaggtg ctgctggacg aggcctatcc ctcgctgggc aagtccgtcg ccgaattcgt
     1561 gcagggttat ctcgacgcgc gcgagaccgt gttgatcctt caggggccgc cgggcacggg
     1621 caagacgcgg ctggtgcgcg cggtgctggc cgcgatgtcg cgccgcaagg gcgacagcgc
     1681 caaggtcatg ttcacggcgg acaagcgcgc gatcgagaac gacgagatct tcgtggactt
     1741 catcaccggg tcgcacgatg cgttcgtgat cgaggacgcg gatcatctgc tgaaggcgcg
     1801 caccagcggc aacttcgagc tgcaccgctt cctgagcgtg gccgatggcg tcgtgcgtgc
     1861 gcagaaccgc aaggtcatct tcacgaccaa cctgccgaac atcaacgaca tcgacgatgc
     1921 attgctgcgg ccgggccggt gtttcgcggt gaagaacctg cggagcatgg atctcgcgga
     1981 ggcggaccgg ctcgtggagc ggctgtgcgg cggcgatgcc gcgcgcctgg cgcgtgcgcg
     2041 ttcatcgctg gagtcggtgg gcgggaagtc gcgctcggtc gcgcaggtct atcgcgcctg
     2101 cgcgtgaggt ctggccccgt tacttgaggt gcaggcggta gaagacgacg tattcgccct
     2161 ggcccggatc cgcctcgagg caccagccct tttcgctctt gctgcgatag aggttctcgg
     2221 cgatccagcc gagcagcggc gtgcggcgaa gctcgggcgt gagcaggcgc gcgcgcatgt
     2281 cgctgttctt cgggaagggc agcggcgtga gtgcgggatc gagatcgagc agcgcggtct
     2341 tctcgagatt gccgggggcg cgcttctttt tcgcgcgggc gtcgagctcg ttgttggtgg
     2401 tgtgcggaat ctcgagaggc gcggcggtct tgtttgactg cctggcggcg tcgtcgccgg
     2461 cggtagcaat gcaaggcacc gccagcaggc agggcgccag caatcccaac gcgaacccga
     2521 acttgcgacc ctcgcggatc atcgaacgac ctcgccagtc tttccgttgc gatcaggtta
     2581 ggagcccgtc cgcatggcta gcaataggtg aatccaccca ggcgcggctt tcgagaaaaa
     2641 agtcgggaat attgtctgat tgcaccgttc cggccgccac attccgccct caaagcgccg
     2701 ccggattgac agcgaaatgt gagccagtcg cacgcctttg gttacactgc agcgcaatga
     2761 cgatagacga acgaatcaac gcgacactgg cgcccatcgc cgactggttg ggaaaagtgg
     2821 tcttcttcgc ggtgcccgtc ggcgaggcac agcttccgct gatcgtcgcg tggctcatca
     2881 tgggcggcgt cgtctgcacc atcgcgttcc ggttcgtgaa tctgcgcggc tttggccact
     2941 cggcgcgcgt catccgcggc gactactcca gcaaatcaca cgccggcgaa gccacgccgt
     3001 tccaggcgct gagcacggcg gtgtcgggca ccgtgggtct cggcagcagc ggcggtgtcg
     3061 ctgtcgcggt cacgctcggc ggccccggcg ccacgcggtg gatgatgctt gccggcttcc
     3121 tcg
//
LOCUS       DATDEU010000255         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2088075, whole genome shotgun sequence.
ACCESSION   DATDEU010000255 DATDEU010000000
VERSION     DATDEU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088075"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VIV63_06190"
     CDS             complement(<1..380)
                     /locus_tag="VIV63_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate pyruvate-lyase family protein"
                     /protein_id="HEY6124220.1"
                     /translation="MRETFERVIADDDSLSLPQKVLLTTDGTVTQLLEIYTGERIRVE
                     KLEHALVTGGPAPLGVSATEPVLSRTILLRGASRPYMYAHSWLVPSRMPRGMQETILT
                     TDTPIGQLWKASRLETFREIIDFRR"
     gene            complement(388..1629)
                     /locus_tag="VIV63_06195"
     CDS             complement(388..1629)
                     /locus_tag="VIV63_06195"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF006587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEY6124221.1"
                     /translation="MSGSEKRVVVTGFGGITALGHDWPTIERALRANVSGIVRMDAWD
                     RYPELNTRLGGPVRPFDLPPQYNRKVLRTMGRVAMLATRATEMALQDAKLEGDPVVGS
                     GEMGVAYGSCTGSTDAIREFGSMLTTGDVSGINATTYLRMMPHTAAANISVYFGIKGR
                     LIPANSACTSGSQSIGYAYEAIRFNRARLMVAGGAEELCASEAAVFDTLFATSTRNDT
                     PTETPSPFDRDRDGLVIGEGACTLVLEELEHARARGAPIYAEIVGFGSNTDGDHPTHP
                     NTQTMERAMRMALDDASLPPDAIGYVNAHATATEQGDIAETQAMQRLFARRIPVSSAK
                     GHLGHTLGACGAVESWFTIQMMRGGWFMPTLNLRNVDDRCGDLDYVTNGGLNLDCEYA
                     MKNNFAFGGVNTSLIYRRWQG"
     gene            complement(1637..>2081)
                     /locus_tag="VIV63_06200"
     CDS             complement(1637..>2081)
                     /locus_tag="VIV63_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6124222.1"
                     /translation="AFPALTDEDWDTVLRTNLDGFYNVLHPAIMPMIRRRAPARIVTM
                     SSVSGITGNRGQVNYSASKAGIIGATKALAVELASRGITVNCVAPGLIRTDMIQDAPV
                     EEALKMVPMGRVGEPGEVAGVINFLMSDEAAYITRQVIGVNGGMV"
BASE COUNT          331 a          685 c          662 g          403 t
ORIGIN      
        1 cgccggaagt cgatgatctc ccggaaggtt tcgaggcggc tcgctttcca taactggccg
       61 atcggcgtgt cggtagtcag gatcgtctcc tgcatgccgc gcggcatgcg cgatggcacc
      121 agccatgaat gcgcgtacat gtacggcctc gaagcaccgc gcaacaggat cgtgcggctg
      181 agcacgggtt ccgtggcgga aacgccgagc ggcgcgggcc caccggtcac cagtgcatgt
      241 tcgagtttct ccacgcggat tctttcaccg gtgtagatct cgagcagttg cgtgaccgtg
      301 ccgtcagtcg tcagcaggac cttttgcggc aggctcaggg agtcgtcgtc ggcgatgacg
      361 cgctcgaatg tctcgcgcac caggtcttca gccctgccag cggcgataga tcagcgaggt
      421 attcacgcca ccgaaggcga agttgttctt catggcgtat tcgcaatcga gattgaggcc
      481 gccattcgtc acgtagtcga gatcgccgca gcggtcgtcg acattgcgca gattgagggt
      541 cggcatgaac cagccgccgc gcatcatctg gatcgtgaac cagctttcga cggcgccgca
      601 cgcgccgagc gtgtggccca ggtggccctt cgcggagctc acgggaatcc tgcgcgcgaa
      661 cagccgctgc atggcctgcg tttcggcgat gtcgccctgt tcggtggccg tggcgtgcgc
      721 attcacgtaa ccgatcgcat cgggcggcag tgacgcatcg tcgagcgcca tgcgcatcgc
      781 ccgctccatg gtctgcgtat tcggatgcgt ggggtggtcg ccgtcggtgt tgctgccgaa
      841 gccgacgatc tccgcgtaga taggcgcacc gcgcgcgcgc gcatgttcca gttcttccag
      901 caccagtgtg caggcgccct cgccgatgac gaggccgtcg cggtcgcgat cgaacgggct
      961 cggcgtttcg gtaggcgtgt cgttgcgcgt gctggtcgcg aacaacgtgt cgaacaccgc
     1021 cgcttcgctc gcgcagagct cttcggcgcc gcccgccacc atgagcctgg cacggttgaa
     1081 tcggatggcc tcgtaggcgt agccgatgct ctggctcccc gaggtgcagg cgctgttcgc
     1141 cgggatgagc cttcccttga tgccgaagta cacactgatg ttggccgccg cggtgtgcgg
     1201 catcatgcgt agataggtgg tggcgttgat gccgctcacg tcgccggtgg tgagcatgga
     1261 accgaattcg cggatcgcat cggtgctgcc ggtgcacgat ccataggcca cgcccatctc
     1321 gccgctgccg acgaccgggt cgccttcgag ctttgcatcc tgcagcgcca tctccgtggc
     1381 gcgcgtcgcg agcatggcga cccggcccat cgtgcgcagc accttgcggt tgtactgcgg
     1441 cggcaggtcg aacggacgca cggggccgcc caatcgcgta ttgagttccg gataccggtc
     1501 ccacgcgtcc atgcgcacga tcccgctgac attggcgcgc agtgcccgtt cgatggtggg
     1561 ccagtcatgc cccagggccg tgatgccacc gaagcccgtc accaccactc gtttttccga
     1621 tccgctcatc gcttcctcaa accattccgc cattgacgcc gatgacctgc cgcgtgatgt
     1681 acgccgcttc gtcggacatc aggaaattga tgactcccgc cacttcgccc ggctcgccca
     1741 cgcgccccat cggcaccatc ttgagcgcct cttccaccgg cgcatcctgg atcatgtcgg
     1801 tccggatcaa tccgggcgcc acgcaattca cggtgatgcc gcgactcgcg agctccaccg
     1861 ccagagcctt ggtcgcgccg atgattccgg ccttggatgc gctgtagttg acctggccgc
     1921 gattgccggt gattcccgag accgacgaca tagtcacgat acgcgccggc gcgcgccgcc
     1981 ggatcatcgg catgatcgcc ggatgaagca cattgtagaa gccgtccaga ttcgtgcgca
     2041 gcaccgtgtc ccagtcttca tcggtaagtg ctggaaaagc g
//
LOCUS       DATDEU010000256         6466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_775409, whole genome shotgun sequence.
ACCESSION   DATDEU010000256 DATDEU010000000
VERSION     DATDEU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775409"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VIV63_06205"
     CDS             complement(<1..338)
                     /locus_tag="VIV63_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY6124223.1"
                     /translation="MSATPQEEEKPPRLPPKADRQFDVGEADDAIHHITELAAIHGDI
                     FRFYSPSQRADLWLINHPDDVKRVLVSNHKNYTKGLGLDRVKILLGHGIMTSEGDLWK
                     RQRYMMQPSF"
     gene            411..1163
                     /locus_tag="VIV63_06210"
     CDS             411..1163
                     /locus_tag="VIV63_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6124224.1"
                     /translation="MPTDPEDAGGLRNTLYKKETRSKRRMTPARRFVWKIVAAIGVFL
                     LKSFWRTCRVVRVVGDEHTAAALAASPSLIPVYWHQHQMFCAKYLLEQRSRGLKLGFL
                     ISPSVDGDIGVMVVSYFGGHSIRGSTTHTGARALRDYYDALVKQGISPSITPDGPKGP
                     RFVFKPGAILLSQMSQRPILPMAWCASRATLFAWDKFVLPWPFSRIVIAVGPPRQVPR
                     TMQPAALEAMQLEMAAELNRLFGVARDSLSAP"
     gene            1160..1885
                     /locus_tag="VIV63_06215"
     CDS             1160..1885
                     /locus_tag="VIV63_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6124225.1"
                     /translation="MSLDFSVVVPVCNEEENVEPLAREIDAAMNSRSYEMIFVDDGST
                     DNTVAILKKLKDSLPALRVLRHSFRSGQSAAVASGVRAARAPWIATLDGDGQNDPADI
                     PRLIASRDSPEGRGVQLFMGNRKASRKDTAFRKLQSNIANSVRSGLLGDGTPDTGCGI
                     KLFSREVFMDLPRFDHMHRFLPALFQRHGARVISVPVSHRARMRGTSKYGMLNRLWVG
                     IVDIIGVMWLRRRYKSGLLVRED"
     gene            1908..2216
                     /locus_tag="VIV63_06220"
     CDS             1908..2216
                     /locus_tag="VIV63_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6124226.1"
                     /translation="MSGVIGVYFGVVLTPWKLVGFLGALMFAGRWVIQAWATKRAGRP
                     MIPRSFWIISLMGSAMVTSYFIWGKNDSVGVLTNLLPASVAFYNLVMDIRAGRAGQHA
                     "
     gene            2293..4299
                     /locus_tag="VIV63_06225"
     CDS             2293..4299
                     /locus_tag="VIV63_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY6124227.1"
                     /translation="MQPAVVAREVAAPFLAPVDRGIAVATAVVSTRLPAEPEAVEPQT
                     AVPEAVVPEAVETAPATVPEIAAAIPTEAASPPPAPATPFQPSGGKGAPAMPSQPAGV
                     PPIRLRDLLWLALAFLIIIGTGLGIRDPWPADEPRFASLARDMALSGEWMFPRVGGDL
                     YQDKPPLYFWLLAICYSLFGSFKGWFLIPSFLAAGGTLFLIYDFGRRSVSREAGLAAA
                     LISVCTLQFVMVMRGAQIDATLCFLTTFSLYALLRHLLLGPAWRWYFIGGFVAGLGVF
                     TKGVGFLPVLLLIPFFSLRALRWKGLPALDAGAGGWRWWLAPVAMLIAISLWFVPMLL
                     TVAASNSAEYAAYRDEILFKQTVGRYAAAWHHVKGWYYFILEVIPALWLPWSLLLIWL
                     APRFKAAFQERNARVWLPLFWALLVLVFFSASPGKRGIYIFPALPALAFAAMPFLESL
                     LTRAGVRRAGFVLAGFFFVAAAALAVGFAVEAKFAMKAIEAVGPRIVMAIHLYLVLCA
                     AGLVYAFLRAPLAAWPVALASLAIVFSYVIAPSMDGERSGKDFTQGVLGQLKPGESLA
                     LVAYKEQFLLYLDRPTINFGHRRWLEGPQESYDSSQWLNAAPNRVLLVPESTLKKYPC
                     FLVAVTPAGRASSEDWFLVRAPAERSCAEQGDSSRAISYAAAGR"
     gene            4479..>6466
                     /locus_tag="VIV63_06230"
     CDS             4479..>6466
                     /locus_tag="VIV63_06230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6124228.1"
                     /translation="MAQAPEHPGDLTASDVFATGVWSARGGKPTLGVDIACTKIAIDL
                     QNMRRGSIESCIRESLNSLRDSSGVDTAFVWLFSPDGSTIDEVYSAHGALAQCRVEAW
                     RSATIDTYPWLKGRLDHLRLSEIRDSAAPRREQLIESQRLMELQIGSVLMISFRVGGV
                     QAGILGLANALPRGAWDVNLQLLLKLLGNSMAAGLERLRVEGRLHKLEERLELAQGAA
                     NDGLWDFDIESNEVHFSPRWKQMLGYPVDALLDSPDWRSLVHPEDMSRVQSAIRDHVA
                     GKSPIFESVHRMKHRNGDWRWVVSRAKARVDEHGRLLRLVGVELDITERKLYEEALFR
                     EKESAQITLQSIGDGVITTDANSTVDYINPVAEQLTGWRLEDAMGRPIEEIYRAFHEE
                     TCEPLENPLSVSIRRTRPIKSVRPMLLIRRDGNELYVESTAAPIRDGAGAVSGGVLVF
                     HDVTESRELNRRLSYHASHDLLTGLVNRREFESRVERALKSAKARESSYALCYLDIDQ
                     FKIINDTCGHSAGDVLLGQVGALLKSKVRWRDTLARLGGDEFGILLEACSLDEALRTA
                     EVLRESVRNFRFTWEDRVFRLGASVGVVPIAADNEDVASIISAADSACAAAKEGGRNR
                     VHSFAENDIELMRRRREMQWAARINAALEEGRFELYRM"
BASE COUNT         1045 a         2063 c         2087 g         1271 t
ORIGIN      
        1 tggaacgaag gctgcatcat gtagcgctga cgtttccaga ggtcgccttc gctggtcatg
       61 atgccgtggc cgagcaggat cttgacccgg tccagcccta atcccttggt gtagttcttg
      121 tggttcgaca ccagtacgcg cttgacatca tcgggatggt tgatgagcca caggtccgcg
      181 cgttgcgacg gggagtagaa acggaagata tcgccgtgga tggcggcaag ttcggtgatg
      241 tggtggatgg cgtcgtcagc ttcgccgacg tcgaactgtc tgtccgcctt cgggggcaaa
      301 cgcgggggct tttcttcttc ctggggggtc gcggacatgt gcccggattg taattgcagc
      361 gataggccga ttcatcgaca attcgcgcct cttttgccgg cctcatccgg atgccaacag
      421 atcccgaaga cgcaggcgga ctgcgcaaca ccctctacaa gaaggagacc cgatcgaaac
      481 gtcggatgac tcctgcgcgt cgttttgtct ggaaaatcgt cgcggcgatc ggggtgttcc
      541 tgctcaagtc gttctggcgc acctgccgcg tggtgcgtgt ggtcggcgac gagcacacgg
      601 cggctgcgct ggctgcttcg ccgtcgctga tcccggtcta ttggcaccag caccagatgt
      661 tctgcgcgaa atacctgctc gagcagcgat cgcgcggcct gaagttaggc ttcctcatca
      721 gtccttcggt cgatggcgac atcggcgtga tggtcgtgtc gtatttcggg ggccattcca
      781 tccgcggctc caccactcac accggcgcac gcgccctgcg cgattactac gacgcgctgg
      841 tgaagcaggg catatctccc agcatcacgc cggacgggcc gaaaggtccg cgattcgtgt
      901 tcaagcccgg cgccatcctg ctgtcgcaga tgtcgcagcg gccgatattg cccatggcct
      961 ggtgcgcgag ccgcgccacg ttgtttgcct gggacaagtt cgtgctgccg tggccgttct
     1021 cacgcatcgt gatcgcggtg ggtccgccgc gccaggtacc gcgcacgatg cagcccgcgg
     1081 cgctcgaagc catgcagctg gaaatggccg ccgagctcaa tcggctgttc ggcgtggcgc
     1141 gcgattcgct gagtgcgcca tgagcctcga tttttcggtc gtggtgccgg tgtgtaatga
     1201 agaggagaac gtggagccgc tggcgcgcga aatcgacgcc gccatgaatt cgcgttcgta
     1261 tgaaatgatc ttcgtggacg atggcagcac cgacaacacc gtggccatcc tgaagaagct
     1321 caaggactcg ctgccggcgc tgcgtgtctt gcgccactcg tttcgcagcg ggcagagcgc
     1381 ggcggtggcc agcggcgtgc gcgcggcgcg cgcgccgtgg atcgccacgc tcgacggcga
     1441 tggccagaac gatccggccg acattcccag gctcatcgct tcgcgcgatt cgcccgaggg
     1501 ccgtggggtt cagctgttca tgggtaaccg caaggccagc cggaaagaca cggcgtttcg
     1561 caagctgcag tcgaacatcg ccaacagcgt gcgcagcggg ttgttaggcg acggcacgcc
     1621 cgacacgggc tgcggcatca agctcttctc gcgcgaagtg ttcatggacc tgccgcgctt
     1681 cgaccacatg catcgcttcc tgccggcgct gttccagcgc cacggcgcgc gcgtcatctc
     1741 ggtgcccgtg agtcaccgtg cccgcatgcg gggcacgtcg aaatacggaa tgctcaatcg
     1801 cctgtgggtg ggcatcgtcg acatcatcgg cgtcatgtgg ctgcggcggc gatacaagtc
     1861 cggcctgctg gtgcgcgaag actaaaaatg atgaggagcg tcccatgatg agcggcgtga
     1921 ttggcgtcta tttcggtgtc gtgctgaccc cgtggaaact cgtgggcttc ctcggcgcgc
     1981 tgatgttcgc gggccgctgg gtcatccagg catgggccac gaagcgcgcg ggccggccga
     2041 tgatcccgcg cagtttctgg atcatcagcc tcatgggcag cgccatggtc acgtcgtatt
     2101 tcatctgggg caagaacgac tcggtcggcg tgctcaccaa tctgctgccg gcctcggtcg
     2161 ctttctacaa cctcgtgatg gacatccggg cgggtcgcgc cggtcaacat gcctgatatc
     2221 ggtcttctcc agggtggcgc gccgaagccg cgcgccgttc cgcctgtcaa agtttctgtg
     2281 ccggatgatg acgtgcagcc agccgtggtc gcgcgcgaag tcgcggcacc gttcctggcg
     2341 cccgtggatc gcggaatcgc ggtggccact gcggtagtta gcacgcggct gccagcagaa
     2401 cccgaagccg ttgaaccgca gacggccgtc ccggaagccg tcgtaccgga agccgtcgag
     2461 accgcacctg cgacagtccc cgagatcgcg gcggccatcc cgacggaagc cgcgtcacca
     2521 cctcccgcgc cggccactcc tttccagcca tcaggcggga agggcgcgcc agcaatgcca
     2581 tcacaaccgg caggcgtgcc tccgatccgc ctgcgcgatc tgctgtggct ggcgctggcc
     2641 ttcctcatca tcatcggtac cggtctcggc atccgcgacc cgtggcccgc tgacgaaccg
     2701 cggtttgcct cgctggcgcg cgacatggcg ctttcgggcg agtggatgtt cccgcgcgtc
     2761 ggcggcgacc tctaccagga caagccgccg ctgtacttct ggttgctcgc catctgttac
     2821 tcgttgttcg gctcattcaa aggttggttc ctcattccat cttttctcgc cgcgggcggc
     2881 acgttattcc tgatctacga cttcggccgc cgctcggtca gccgcgaggc gggcctcgcc
     2941 gccgcgctga tcagcgtgtg cacgctgcaa ttcgtgatgg tcatgcgcgg tgcgcagatc
     3001 gacgcgacgt tgtgcttcct caccacgttc tcgctgtatg cgctgctgcg tcacctgctg
     3061 ctgggccccg catggcgttg gtatttcatc ggcggcttcg tcgcggggct gggtgtgttc
     3121 accaagggcg tcggcttcct gcccgtgctg ctgctgattc ccttcttctc gctgcgcgcg
     3181 ctgcgctgga aagggctgcc cgccctcgac gcgggcgcag gtggctggcg ctggtggctc
     3241 gcgccggtcg cgatgctgat cgccatctcg ttgtggttcg ttccgatgct gctcacggtg
     3301 gcggccagca attcggcgga gtacgccgcg tatcgggacg agatcctctt caagcaaacc
     3361 gtcgggcgtt acgccgcggc ctggcatcac gtcaaaggct ggtactactt cattctcgaa
     3421 gtgatccccg cgctctggct gccgtggagc ctgctgctca tctggctggc gccgcgattc
     3481 aaggccgcat tccaggaacg caacgcgcgc gtgtggctgc cgctgttctg ggcgctcctg
     3541 gtgctggtgt ttttctcggc gagccctggc aagcgcggca tctacatctt cccggcattg
     3601 ccggcgctgg cttttgcggc gatgccattc ctcgaatcgt tgctgacacg cgccggcgtg
     3661 cggcgtgcgg gcttcgtcct ggcaggtttc ttcttcgtcg ccgcggccgc gctcgcggtg
     3721 gggtttgccg tcgaggcgaa attcgcgatg aaggccatcg aggcggtcgg cccccgcatc
     3781 gtcatggcga tccaccttta tctcgtgctg tgcgcggcgg ggctcgtcta cgccttcctg
     3841 cgtgcgccac tggcggcgtg gccggtggca ttggcttcac tcgccatcgt cttctcctat
     3901 gtcatcgcac catcgatgga tggcgaacgc tcgggcaaag acttcacgca gggcgtgctc
     3961 gggcaactga aacccggaga gtcactggcg ctggtcgcct acaaggaaca gtttctgctg
     4021 tacctcgatc ggccgaccat caacttcggc catcgtcgct ggctcgaagg cccgcaagag
     4081 tcctacgact cgtcgcagtg gctcaacgcg gcgccgaatc gagtgttgct cgtgccggaa
     4141 tccacgctga agaagtaccc gtgtttcctg gtggcggtga cgccggcggg ccgcgcatcg
     4201 agcgaagact ggttcctcgt gcgtgctccg gcggagcgtt cctgcgcgga acagggcgat
     4261 tcatcacgtg ccatctcata cgcggccgcg ggacgttgag acttaacact gttcgaatct
     4321 tgagcaattg cgattcattc tctcgacgga ccatgcactt agcttgtgtt cttcgattct
     4381 tatgacagaa tgcatcgcaa ctttgggagg aggctggcgt tggaatctgt ggcccggttc
     4441 ggagcggcac atggatctac gctgtagctt gcaaaagagt ggctcaagca cctgaacatc
     4501 ctggtgatct gaccgcatcc gacgtgttcg ccacaggcgt gtggtcggcg cgcggtggaa
     4561 agccgacgct gggcgtcgat atcgcctgca ccaagatagc catcgacctg cagaacatgc
     4621 gtcgcggctc catcgagagc tgcatccgcg agagcctcaa ttcccttcgt gattcctcgg
     4681 gcgtcgatac cgccttcgtg tggctgttct cgccggatgg ctcgacgatc gatgaggtgt
     4741 actccgccca tggcgcgctc gcgcaatgcc gcgtggaagc ctggcgcagc gccaccatcg
     4801 acacctaccc ctggctcaag ggccgcctcg accaccttcg cctctccgaa atccgcgaca
     4861 gcgccgctcc gcgccgcgaa cagctcatcg agagccagcg cctcatggag ctgcagatcg
     4921 gatcggtgct catgatttcg ttccgcgtcg gcggcgtgca ggccggcatc ctcggcctgg
     4981 cgaacgcgtt accgcgtggc gcctgggacg tgaacctcca gctcctgctg aagctgctcg
     5041 gaaattcgat ggccgccggt ctcgagcgcc tgcgcgtcga gggccgtctg cacaagctgg
     5101 aagaacgtct ggaactcgcg cagggcgcgg ccaacgacgg cttgtgggac ttcgacatcg
     5161 agagcaacga agtccacttc tcgccgcgct ggaaacagat gctcggctac ccggtggacg
     5221 cgttactgga ttccccggac tggcgcagcc tcgtgcatcc cgaagacatg tcgcgcgtgc
     5281 aatcggccat ccgcgatcac gtcgccggca agtcgccgat tttcgaaagc gtgcatcgca
     5341 tgaagcaccg caacggcgac tggcgctggg tggtgagccg cgccaaggcg cgcgtggatg
     5401 agcacggacg gctgttacgt ctcgtcggag tcgagctcga catcaccgaa cgcaagctct
     5461 acgaagaagc gctgttccgc gagaaggaaa gcgcgcagat cacgctgcag tccatcggtg
     5521 acggcgtcat cacgacggat gcgaactcca cggtggatta catcaatccc gtggccgagc
     5581 agttgaccgg ctggcgcctc gaagacgcca tgggccggcc gatcgaagaa atctaccgcg
     5641 cgttccacga agaaacctgc gagccgttag agaatccctt gagcgtctcg atccgccgca
     5701 cgcgtccgat caagagcgtg cgcccgatgc tgctgatccg ccgcgacggc aatgagctgt
     5761 acgtcgagag caccgcagcg cctattcgcg acggcgcggg cgcggtatcc ggcggcgtgc
     5821 tcgtgttcca tgacgtgacg gagtcgcgcg agctgaatcg ccgcctctcg tatcacgcgt
     5881 cgcacgactt gctgacgggc ctcgtgaacc gacgcgaatt cgagagccgc gtcgaacgtg
     5941 cgctcaagag cgccaaggcg cgcgagtctt cgtatgctct gtgctacctg gacatcgacc
     6001 agttcaagat catcaacgac acctgcgggc actcggccgg cgacgtgttg ttaggccagg
     6061 tcggcgcgct gctgaagtcg aaggtccgct ggcgcgatac gctcgcgcga ttgggcggtg
     6121 acgaattcgg catcctgctc gaggcctgtt cgctggatga agccctgcgc acggccgaag
     6181 tgctgcgcga gtccgtccgc aatttcagat tcacctggga agatcgcgtg tttcgtctgg
     6241 gcgcatccgt cggcgtggtg ccgatcgccg ccgacaacga agacgtggca tcgatcatct
     6301 cggccgcgga cagcgcctgc gctgccgcga aagaaggcgg ccgcaaccgc gtgcacagct
     6361 tcgccgagaa cgacatcgaa ctcatgcgcc gccgccgcga gatgcagtgg gccgcgcgta
     6421 tcaacgccgc gctggaagag ggccgcttcg agctgtatcg catgca
//
LOCUS       DATDEU010000257         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_336643, whole genome shotgun sequence.
ACCESSION   DATDEU010000257 DATDEU010000000
VERSION     DATDEU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336643"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..847)
                     /gene="otsB"
                     /locus_tag="VIV63_06235"
     CDS             complement(83..847)
                     /gene="otsB"
                     /locus_tag="VIV63_06235"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY6124229.1"
                     /translation="MMQLAERDAAAVHAVPQPAGHWCLFLDVDGTLLELADHPQAVAV
                     QPHLMPLIERLRAAANGAVALVSGRTISDLDSLFGHPELPIAGLHGCERRDARGQLHV
                     AAIAREQLADVRDGLLRLIKRHTGLLLEDKGAGLALHFLKAPHLEHELRAEVALLAAP
                     LVPRFTVLGGHAVLEVKPAAHTKDSAVSAYLLEEPFRGRMPIFIGDDQTDYDGFAAVR
                     RANGMAIAVGPRVRSEYWLPGPAAVHHWLEQLAEHS"
     gene            complement(968..2260)
                     /locus_tag="VIV63_06240"
     CDS             complement(968..2260)
                     /locus_tag="VIV63_06240"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY6124230.1"
                     /translation="MGKFVIIIGTQWGDEGKGKVVDLLTERAAAVARFQGGHNAGHTL
                     VIGGKKTVLSLIPSGILHKNVQCLIGNGVVLSLEALFRESQTLIDTGVDVFARLRISP
                     NCPLILPSHVALDKAREVARGANAIGTTGRGIGPAYEDKVARRAVRVQDLFQREKFAA
                     KLGEILDFHNFTLQHYFKQPAVDFQKTLDEQLSYADRVRPLVSDITQTLASLREKKAD
                     VMFEGAQGAMLDVDHGTYPYVTSSNTTGGFAATGTGLGPRAFDYVLGIVKAYTTRVGG
                     GPFPTELFDEYGEHLSRVGHEFGAVTGRKRRCGWFDAVALRRSIINSSVSGLCLTKLD
                     VLDGLDTLQICVGYKSDGKVSENPPLFADAFAEVEPVYEEMPGWKESTIGVTSYDALP
                     LNARNYLGRLQQLAGVPIDIISTGPDREQTIVLRHPFD"
BASE COUNT          388 a          735 c          753 g          388 t
ORIGIN      
        1 ggacgcggca ggcacgccca caccatgcga gcctgctcgt cgatgagcgc cgccacctgg
       61 caattgccga tcacgccgta gttcacgaat gctccgcgag ctgctcgagc cagtgatgca
      121 ccgcggcggg accgggcagc cagtactcgg atctcacacg gggacctaca gcaatggcca
      181 tgccgtttgc gcgcctgacg gctgcaaacc cgtcgtagtc ggtctggtcg tcgccgatga
      241 agatcggcat gcgtccgcga aagggttctt ccaggagata ggcggacacg gcgctgtcct
      301 tggtgtgcgc cgcgggcttc acttcgagca ccgcgtggcc gccgagcacg gtgaaacgcg
      361 gcaccagcgg cgcggcgagt aacgcgactt ctgcgcgcag ctcgtgttcg aggtgcggcg
      421 ccttcaggaa atgcagcgcg aggcccgctc ccttgtcctc gagcaggagg cccgtgtggc
      481 gtttgatgag tcgcaggagg ccgtcgcgaa cgtcggcgag ctgctcacgc gctatcgccg
      541 ccacgtgcaa ctggccgcgc gcatcgcgcc gctcgcagcc gtgcagcccc gcgatcggca
      601 gttccgggtg gccgaacagg ctgtcgagat cggagatggt tcggccgctg acgagcgcca
      661 cggcgccgtt tgccgcggcg cgcaaccgct cgatgagcgg catgaggtgg ggctgcaccg
      721 ccacggcctg cgggtgatcg gccagttcga gcagtgtccc gtcaacgtcg agaaacaggc
      781 accagtgacc cgcgggctgc ggcaccgcgt gcacggcggc cgcatcgcgc tctgccaatt
      841 gcatcatggg cgcagtataa gaaccgcgac gcgcccgttg cggtaaatct cgggtagtta
      901 ggggtgagac ttttcctgca agcggacgtg aacttcggct gccgtcagac ggcgcctaca
      961 cgaagcttca gtcgaacgga tgccgcagca cgatcgtctg ctcgcgatcg gggcccgtgg
     1021 agatgatgtc gatgggcacg cccgccagtt gctgcaggcg cccgagatag ttgcgcgcat
     1081 tcagcggcag cgcgtcatac gaggtgacac cgatggtgga ttccttccag cccggcatct
     1141 cttcgtacac cggctccacc tccgcgaacg catcggcgaa caacggcgga ttctctgaaa
     1201 cctttccatc ggacttgtaa cccacgcaga tctgcagcgt gtcgagcccg tcgagcacgt
     1261 cgagcttggt caggcacagc cccgacacgc tcgaattgat gatggagcgt cgcaacgcga
     1321 cggcatcgaa ccatccgcag cggcgcttgc ggcccgtgac cgcgccgaat tcatggccga
     1381 cccgagagag atgctcgccg tattcgtcga acagctccgt ggggaacgga ccaccgccca
     1441 cgcgcgtggt gtaagccttc acgatgccga gcacgtaatc gaacgcacgc ggtcccaggc
     1501 ccgtgccggt ggccgcgaat ccgccggtcg tattggagga cgtgacgtac ggataggtgc
     1561 cgtggtcgac gtcgagcatc gcgccctgcg cgccttcgaa catcacgtcg gccttcttct
     1621 cgcgcagcga cgccagcgtc tgcgtgatgt cgctgaccag cgggcgcacg cgatcggcgt
     1681 acgacaactg ctcgtcgagt gtcttctgga aatcgaccgc cggctgcttg aagtagtgct
     1741 gcagcgtgaa attgtggaag tcgaggatct cgccgagctt cgcggcgaat ttctcgcgct
     1801 ggaagaggtc ctgcacgcgc accgcgcggc gcgccacctt gtcttcgtag gccggaccga
     1861 tgccgcgccc ggtggtgccg atggcattgg cgccgcgcgc cacttcgcgc gccttgtcga
     1921 gcgccacgtg cgacggaagg atcagcggac agttgggcga aatccgcagg cgcgcgaaca
     1981 cgtccacgcc ggtatcgatg agtgtctgcg attcgcggaa cagcgcttcg agcgacaaca
     2041 cgacgccgtt gccgatgagg cattggacgt tcttgtgcag gattcccgaa gggatgagcg
     2101 acaacacggt cttcttgccg ccgatgacca gcgtgtgacc ggcgttgtgt ccgccctgga
     2161 agcgcgccac cgcggccgcg cgttcggtga gcaggtcgac gaccttgccc ttgccctcgt
     2221 caccccattg ggtgccgatg atgatgacga atttgcccat ggtt
//
LOCUS       DATDEU010000258         3999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1504868, whole genome shotgun sequence.
ACCESSION   DATDEU010000258 DATDEU010000000
VERSION     DATDEU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504868"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VIV63_06245"
     CDS             <1..896
                     /locus_tag="VIV63_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY6124231.1"
                     /translation="IYKVLVFLMTPVTWFTNKAAYGFLRLFGVKAATEGDQSMSPDEL
                     RSVVAEAGPMIPTRHRQMLLSILDLEHVTVNDIMIPRQEIASINADDNWEDILDQLRQ
                     TPHTRLPLYEGDLDNLVGIVHMKRVAQELARDTLSRERLIELARTREPYFVPEGTSLT
                     VQLNQFQRNRRRMAFVVDEYGDIQGFVTLEDILEEIVGEFTTDPATITHKHVHREAPG
                     VFIVNASATIRALNRSLGWQLPVDGPKTLNGLLVEQLETLPEAGDRVEVGEYQFEVLQ
                     IGENTIRTVRARRAAAPAAAG"
     gene            complement(897..2243)
                     /gene="radA"
                     /locus_tag="VIV63_06250"
     CDS             complement(897..2243)
                     /gene="radA"
                     /locus_tag="VIV63_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY6124232.1"
                     /translation="MAKPAIVFVCAECGAETLKWQGQCPQCAQWNTLEQRTVARRATA
                     QTAPLAASTDLAELGTAELPRLQTGMGELDRVLGGGLIPGSVTLLGGEPGIGKSTLLL
                     QLANHAALVTPTLYLSAEESAAQIGLRARRLGVAGSHLRVVADTDLEAMMARASSEGV
                     KLLIVDSIQTVQLSSLGTAAGAITQLKECTAQLVRFAKSSGVSVVIVGHVTKDGAIAG
                     PRVLEHLVDTVLYFESDASSRFRNVRATKNRFGSVGELAFFHMGESGLREVRNPSAIF
                     LARSGVPAPGSVVAVTRDGRRPLLVELQGLVDRMRFGSPRRVAQGLDAQRVAMLLAVM
                     NRHGGVSLQEYDVFVNLVGGLEVTETACDLPVALALASSLADKPIAQDLVAFGELGLT
                     GELRPVAYGEERLREAAKQGFTTALIPKDNAPRTPIEGLRVREFTRIDDVLKAALG"
     gene            2563..3387
                     /locus_tag="VIV63_06255"
     CDS             2563..3387
                     /locus_tag="VIV63_06255"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEY6124233.1"
                     /translation="MSLKGKLSFVGQTDTGRVREHNEDTIASDVDVGLLVLADGMGGY
                     NAGEVASGIAVKTITNLVREGLAREDLGALDRATGLTRPSIVLRDAITRANKIIYQTA
                     RSQAECEGMGTTVVAALFYDNKISIAHVGDSRLYRQRGSQIAQVTMDHSLLQELVDRG
                     FYSPEEAQRAANKNYVTRALGVEPQVEVEVQEHPVDKGDIFILCSDGLSDMVEDEDIR
                     LTISTFGANLDTVAKQLIQLANENGGRDNVSVVLAQAVEPFPASRGVMDKLLGWFS"
     gene            3400..>3999
                     /locus_tag="VIV63_06260"
     CDS             3400..>3999
                     /locus_tag="VIV63_06260"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY6124234.1"
                     /translation="MARLVLSLDNQVLAEYNMNKERYTIGRLPDNDIRIDNSAVSGHH
                     SLIINILNDSFLEDLNSTNGTYVNGKLIKKHAMQHGDVVTVGHHQLRFVEDDEAQDEF
                     EKTMVIQPSSRPAAEKMQAAKDRAEKVSADVSASGVRKGLDGKTPVKKAKLQVLSGSF
                     AGRELELNKALTTLGRPGIQVAAITRRQDGFFIVHVDSDT"
BASE COUNT          773 a         1285 c         1254 g          687 t
ORIGIN      
        1 acatctacaa ggtgctcgtc ttcctgatga cgcccgtcac gtggttcacg aacaaggcgg
       61 cctatggatt tcttcgattg ttcggcgtga aggcggcgac cgagggcgac caatcgatgt
      121 ctcccgacga actgcgctcg gtggtcgccg aggccggacc gatgatcccg acgcgccacc
      181 gccagatgct gttgtcgatc ctcgatctcg agcacgtgac ggtgaacgac atcatgatcc
      241 cgcgacagga gatcgcgtcg atcaacgccg acgacaactg ggaggacatc ctcgaccagc
      301 ttcgccagac gccgcatacg cgcctgccgc tatacgaagg cgatctcgac aacctggtcg
      361 gcatcgtcca catgaagcgc gtcgcgcagg agctggcgcg cgacactctg tcgcgcgagc
      421 gcctcatcga actggcgcgc acgcgtgagc cgtacttcgt gcccgaaggc acgtcgctga
      481 ccgtgcaact caaccagttt cagcgcaacc ggcgccgcat ggcgttcgtc gtcgacgagt
      541 acggcgacat ccagggtttc gtcacgctcg aggacatcct cgaagagatc gtcggcgagt
      601 tcaccaccga cccggccacg atcacgcata aacacgtgca tcgcgaggcg ccgggggttt
      661 tcatcgtcaa cgccagcgcc accatccgcg cgctcaaccg gtcgctcgga tggcagttgc
      721 cggtcgacgg acccaagacg ctcaacggcc tcctggtcga acagctggaa acgctgccgg
      781 aagccggcga ccgcgttgaa gtgggcgagt accagttcga ggttctgcag ataggcgaaa
      841 acaccattcg cacggtgcgc gcgcgtcgag ccgcggctcc cgcggcggcc ggctagtcag
      901 ccgagcgcgg ccttcagcac gtcgtcgatg cgcgtgaact cccgcacgcg cagcccttcg
      961 atgggcgttc gcggtgcgtt gtccttcgga atgagcgcgg tcgtgaatcc ctgtttggcg
     1021 gcttcgcgca gtcgctcttc gccgtatgcc accggacgca attcgccggt gagcccgagt
     1081 tcgccgaagg cgaccaggtc ctgcgcgatg ggcttgtcgg ccaggctcga cgccagcgcc
     1141 agcgccaccg gcagatcaca cgccgtctcc gtgacttcga ggccgcccac caggttcacg
     1201 aacacgtcgt attcctgaag cgacacgccg ccatgccggt tcatgactgc cagcaacatc
     1261 gcgacgcgct gcgcgtccag accctgcgcc acgcgccgcg gagacccgaa gcgcatgcgg
     1321 tcgaccagcc cctgcagctc gaccagcagc ggacgccggc catcgcgcgt caccgccacg
     1381 acactgcccg gcgccggcac gcccgagcgc gccaggaaaa tggccgacgg gttgcgtacc
     1441 tcgcgcagac ccgattcgcc catatgaaag aacgcgagtt cgccgaccga gccgaagcgg
     1501 tttttggtgg cccgcacgtt gcggaagcgg ctggacgcat cgctctcgaa ataaagcacg
     1561 gtgtcgacca ggtgctcgag cacacgagga ccggcgatgg cgccgtcctt ggtgacgtga
     1621 ccgacgatga ccacactcac gccggaactc ttggcgaagc gaaccagttg cgcggtgcat
     1681 tccttgagct gcgtgatggc cccggcggct gtgccgagcg agctgagctg cacggtctgg
     1741 atggaatcga cgatgagcag cttcacaccc tccgagctgg ctctcgccat catggcctcc
     1801 agatcggtgt cggcgacgac gcgcaggtgt gacccggcaa cgccgagccg gcgggcacgc
     1861 aagccgatct gcgccgccga ctcctcggcc gacaggtaga gcgtgggcgt gacaagcgcc
     1921 gcatggttgg cgagctgcaa aagcagcgtg gatttgccga tgccgggctc gccacctagc
     1981 aacgtgaccg agccggggat gagacccccg cccaggaccc ggtccagttc ccccatgccg
     2041 gtctgcaagc gcgggagctc agccgttccg agttcggcca ggtccgtcga cgccgccagc
     2101 ggcgccgtct gggctgttgc ccggcgggcc accgtacgtt gttccagcgt gttccattgc
     2161 gcgcactgcg ggcactgacc ctgccatttg agggtctcgg ccccgcactc ggcgcagacg
     2221 aacacgatcg ccggtttggc catcctaaaa ccctcgggcg cgagccccgg aaagcgtgac
     2281 gaagttcatg ccttggaagt cgtcggagtc ggtgaggcac ctagtgtgca gtagtccggt
     2341 gcgaagcagc cctaatccca ttagcattgc gtccctaggt cgttgatttg ccgatgtgcg
     2401 agggtgtgaa gtgtgactta ttgcgaaggc ggtcgccgtc gctgctggta ggttacgacc
     2461 cttgcgggga agtctacgga cgcgaatcgg ccgtataggt ctttttttgg gcattggtgt
     2521 gatcgaagaa cacgtcgaac aactccggtt gctgggtcac ccatgagcct caaggggaaa
     2581 ctgtcctttg tcgggcagac cgacacgggt cgcgtccgcg aacacaacga ggacaccatc
     2641 gcgtcggacg tcgacgtggg cttgttagtt ctcgctgacg gcatgggcgg atacaacgcg
     2701 ggcgaagtcg cgagcggcat cgccgtcaaa accatcacca acctggttcg tgagggtctt
     2761 gcgcgcgagg atcttggcgc gctggatagg gccaccggcc tcacccgtcc ttccatcgtg
     2821 cttcgcgacg ccatcacgcg cgccaacaag atcatctatc agaccgcccg gtcccaggcc
     2881 gagtgcgaag gcatgggcac gaccgtggtc gccgccctct tctacgacaa caagatttcg
     2941 atcgcgcacg tgggcgactc gcggctgtat cgccagcgcg gcagccagat cgcgcaggtc
     3001 acgatggacc actcgctgct ccaggagctc gtggaccgcg gcttctactc gccggaagaa
     3061 gcccagcggg cagccaacaa gaactacgtg acccgggcct tgggcgtcga accccaggtc
     3121 gaggtcgaag tgcaggagca tccggtcgac aagggcgata tcttcattct gtgttcggac
     3181 ggcttgtcgg acatggtcga agatgaagac atccgtttaa ctataagtac ctttggtgct
     3241 aatctagata cggttgctaa gcagttgatt cagttagcca acgagaacgg tggtcgtgac
     3301 aacgtctcgg tggtgctcgc gcaggcggtt gaaccgtttc cggcgagtcg cggagtgatg
     3361 gataaactac ttggctggtt cagttaaggg acgaaaacaa tggcacgtct ggtcttaagc
     3421 ctggataacc aggtcctcgc cgaatacaac atgaacaagg agcggtacac cattggccgg
     3481 ctccccgata acgacatccg catcgataac tcggcggtgt cgggtcatca ctcgctgatc
     3541 atcaacatcc tcaacgattc gtttctcgag gatctgaaca gcaccaacgg cacgtacgtc
     3601 aacggcaagc tcatcaagaa gcatgcgatg cagcacggcg acgtggtcac cgtcggtcat
     3661 caccagctgc ggttcgtcga agacgacgaa gcccaggacg aattcgaaaa gaccatggtc
     3721 atccagcctt cttcgcgccc cgcggccgag aagatgcagg cggccaagga ccgtgcggag
     3781 aaagtctcgg cggatgtctc cgcgagcggc gtgcgcaagg gtctcgacgg caagacgccc
     3841 gtcaagaaag ccaagctgca ggtgctctcc ggttccttcg cgggtcgcga actcgaactg
     3901 aacaaggcgc tcaccacgct cggccgtccg ggcatccagg tggccgcgat cacgcgccgc
     3961 caggacgggt tcttcatcgt gcacgtcgac agcgacacg
//
LOCUS       DATDEU010000259         7062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1213166, whole genome shotgun sequence.
ACCESSION   DATDEU010000259 DATDEU010000000
VERSION     DATDEU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213166"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..629)
                     /locus_tag="VIV63_06265"
     CDS             complement(252..629)
                     /locus_tag="VIV63_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6124235.1"
                     /translation="MGTSKNERTGRTGANKPGLLTLTIKDKSALYLAYMPFVKNGGLF
                     IPTNSNYRLGDEVFMLLNLMGEDEKLPVAGRVIWLTPKGAQGKRTAGIGVQFSDQDRG
                     QTQKKIETYLAGALAGDKATHTM"
     gene            complement(569..1564)
                     /gene="holB"
                     /locus_tag="VIV63_06270"
     CDS             complement(569..1564)
                     /gene="holB"
                     /locus_tag="VIV63_06270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY6124236.1"
                     /translation="MNTIPRPPWFDAPCGEVNAAFAADRLAHGLLIHEDPGAGGLEFA
                     RWIAQRVNCREPSRAPCGECQDCRWIAADQHPDVTRLSPEEDSTQIVIHQVRDLSAEL
                     ALTAHGRGYKIAIVSPAEAMNHFAANALLKTLEEPPRRTLVLLVTSQPSRLLPTLRSR
                     CSRLRLPGPTREVAAGFLEAARGAGPWADALSATGAGPFSLLDADPAALAQLRSDTIS
                     TLREIGSGNVQPPAVAERWAKKDDFAIRLSCLESWVTDRILETAPIRDVTHLSGAALP
                     PKICRLFEFSDAIRDMRKLAHTSINKTMAVEALLWRWARQRMREQGVPAPTSPDC"
     gene            complement(1561..2208)
                     /gene="tmk"
                     /locus_tag="VIV63_06275"
     CDS             complement(1561..2208)
                     /gene="tmk"
                     /locus_tag="VIV63_06275"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY6124237.1"
                     /translation="MTPAKFITLEGIEGAGKTTVADRLTQALRARGLTVHATREPGGT
                     KVAERVRALVLERGEEHISATAETLLMFGARQVHVDNLIRPSLTRGEWVLCDRFTDAT
                     HAYQGGGRGVDRKLIDQIAHAVHGDLNPDCTILLDVPVRLGLERMQARRGAVDRFEIE
                     SSQFFDRVRNRYLELARANPARFRVVDATAKLDQVCDDALAALEECLGLPAAAKA"
     gene            complement(2228..3244)
                     /gene="mltG"
                     /locus_tag="VIV63_06280"
     CDS             complement(2228..3244)
                     /gene="mltG"
                     /locus_tag="VIV63_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY6124238.1"
                     /translation="MRKLLWILGLAVVLAGVAAAIYWQRANAAMTADGPHLDSVPLMV
                     KPGTTVRAALAELETRGALADRRAVEIYLRVRGSPQIKTGHYEIPAQASPQEILRQLA
                     EGRVVLESLTVIEGWTFADMRRAVEAHTQINVTLRGKDIAALMTAIGHAGEHPEGRFY
                     PDTYRFAAGTSDRDLYALAYRKMAETLETAWSARAAALPLANAYEALTLASIVEKETG
                     LASERPRIAGVFTTRLRRNMRLQTDPTVIYGLGANYDGNIRERDLRTDTPYNTYTRSG
                     LPPTPIALPSREAIEAVARPLETGDIFFVATGIGDGSHVFSATLEAHNAAVARYLARL
                     RARD"
     gene            complement(3237..4064)
                     /gene="pabC"
                     /locus_tag="VIV63_06285"
     CDS             complement(3237..4064)
                     /gene="pabC"
                     /locus_tag="VIV63_06285"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:NF004761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEY6124239.1"
                     /translation="MSAALGCWIDGIRGEVLPADDRGLQYGDGIFETIVVRAGRPRFL
                     ESHLARLTLGCARLAIPFAALAELRAEIALASSEAPSLAILKIIVTRGSATRRGYAPQ
                     GTETPRRLVSLWPMTAAGEWGSGVKLRVATLRLGENPSLAGIKHLNRLENVLAAAEAC
                     EPDNYESLLLDASGHLVSGAMSNVFIARDGRVATPRIDRCGVSGVMRGIVLRECALLG
                     IAASEERLSLEDLFSADEVFITNARIGVVPVRRVGEHSFGMNTLALRLAAHIEPLDA"
     gene            complement(4061..5419)
                     /locus_tag="VIV63_06290"
     CDS             complement(4061..5419)
                     /locus_tag="VIV63_06290"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF006563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY6124240.1"
                     /translation="MSGEALRSLAASRPAQYPVFFDSAANGPLARFSMLACKPSATLF
                     RDASGRLSAQGRSLVAGGFLDNLEAWFRDEATPATPSATPKALPFVGGWFVYLGYEVA
                     AEIEPRLKLPPADSPFSAFALRVDTFAIHDLGTDRVYAISEKADSATHAELMADLASA
                     HGQPASAGSRLELSALDEEAAELFLARVRAAQEHIAAGDLYQANLSRRWRLKLRHPES
                     AARVYDALRTANPAPFAASVQVQGMAIFSSSPERLLRIEGREISTRPIAGTRARQGSA
                     EQDLRDTAELVAHPKERAEHIMLIDLERNDLGRVCEAGSVTVDEYMVTESYAHVHHIV
                     SNVRGRLRADCSPVDALRALFPGGTITGCPKFRCMQIIAALEGEGRGAYTGSLGWLGT
                     DGDADFNILIRTLTMRDDVIELRAGAGIVADSVPEKELDETRAKARGMLRAFATPAGE
                     TR"
     gene            complement(5451..6698)
                     /gene="fabF"
                     /locus_tag="VIV63_06295"
     CDS             complement(5451..6698)
                     /gene="fabF"
                     /locus_tag="VIV63_06295"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012149092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY6124241.1"
                     /translation="MSKRRVVVTGLGIVSPVGSDVTTAWSSILAGKSGIKPITRIDVT
                     PFATKFAGEVSGFDVATYISPKEARRMDPFMHYGMGAGIQAAADSGLDFAKENGDRCG
                     AIMGSGIGGLAGIEDEAITWERTHNPKKTSPFYIPSTIGNMVAGHLSIRFGLRGPNLG
                     VVSACTTSTHAIGLAMRCIQYGDADIIIAGGSECATTPTALAGFSQAKALSERNDDPE
                     GASRPWDKDRDGFVMGDGGGAVILEELEHAKARGARIYAECIGFGMSGDAYHITAPPE
                     DGAGARLAMVNALRDAQLNPTDIQYLNAHATSTPLGDKAETVAIKHAFGDYAYKMSIS
                     STKSMTGHLLGAAGVVEAIFSILAIRDGVAPPTTNYHTPDPDCDLDYTPNTARKLAIE
                     AALSNSFGFGGTNGSLIFRRFKG"
     gene            complement(6780..7019)
                     /gene="acpP"
                     /locus_tag="VIV63_06300"
     CDS             complement(6780..7019)
                     /gene="acpP"
                     /locus_tag="VIV63_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY6124242.1"
                     /translation="MSTVEQQVKAIVAEQLGVKAEQVVNDASFVDDLGADSLDTVELV
                     MALEEEFEIEIPDEDAEKITTVSQAITYISDRRKS"
BASE COUNT         1191 a         2251 c         2355 g         1265 t
ORIGIN      
        1 aagctaacag cggagcactt gtcagaatcg cgagccagat tgcgaggagc acgcgcatga
       61 ggccatctca caccgtgaag gcggcagata gcgtgattgc cgccgcattc cggccgcgct
      121 gcggagagtt ctgcttcgca attccggcga gtgacagggc ctaatgagaa gcacggcaca
      181 gatctccatg tgccgcccga ctcgcgaatc aggggattgc ggaaaggcgc gacgagcccc
      241 gtggacccgg cttacatcgt gtgcgtggct ttgtcgcccg cgagcgcgcc ggctagatag
      301 gtctcgatct tcttctgcgt ctgcccgcga tcctggtcgg agaactgcac gccgatgccg
      361 gcggtgcgtt tgccctgcgc gcctttcggc gtcagccaga tcacgcgacc ggccacgggc
      421 agcttttcgt cttcacccat gaggttcaac agcatgaaca cttcgtcgcc gaggcggtaa
      481 ttgctgttgg tgggaatgaa cagcccgccg ttcttcacga acggcatgta ggcgagatac
      541 agcgcgctct tgtccttgat ggtgagcgtc agcagtccgg gcttgttggc gccggtacgc
      601 cctgttctct cattctttga cgtgcccagc gccacagcag cgcttctacg gccatggtct
      661 tgttgatgga ggtgtgggca agcttgcgca tgtcgcgaat ggcgtccgag aactcgaata
      721 ggcgacatat cttgggtggc aacgccgccc ctgacaagtg cgttacgtcg cggattggcg
      781 cggtttcgag gatgcgatct gtgacccagc tctcaaggca cgagaggcgt atcgcgaagt
      841 catctttctt cgcccagcgc tcggccaccg cgggcggctg aacgttgccg ctgccgatct
      901 cgcgcagcgt ggaaatggtg tcgctgcgca gttgcgcgag cgccgccggg tcggcatcca
      961 gcagcgaaaa gggccctgcc cccgtggcgg acagtgcgtc cgcccacggc cctgccccgc
     1021 gcgccgcttc gagaaaaccc gcggcgactt cgcgagtggg tccgggcagg cgcaggcgcg
     1081 agcagcggct gcgcagtgtc ggcagcaatc gcgaaggctg gctggtcact aacaacacca
     1141 acgtccgtct gggtggctct tcgagagtct tgagcagcgc gttggcggcg aaatggttca
     1201 tcgcctcggc tggcgagacg atcgcgatct tgtaaccgcg tccatgcgcg gtcagtgcga
     1261 gttcggccga cagatcgcgt acctggtgaa tgacgatctg cgtggaatct tcttcgggcg
     1321 acagccgcgt gacatccgga tgctggtcgg ctgcgatcca gcggcagtcc tggcactcgc
     1381 cgcaaggcgc acgcgagggc tcgcgacaat tcacgcgctg cgcgatccat cgcgcgaact
     1441 cgagtccgcc ggcgcccggg tcttcatgga tcaacaatcc gtgagccagc cggtccgctg
     1501 cgaaggcggc attcacttcg ccacagggtg catcgaacca gggtggccgg ggaatcgtat
     1561 tcaagctttg gccgcggccg gcaggcccag acactcttcg agcgctgcca gggcgtcgtc
     1621 gcagacctgg tcgagtttcg cggtggcatc gacgacgcgg aagcgcgcgg gattggcgcg
     1681 cgccagttcg aggtagcgat tgcgcacccg gtcgaagaat tgcgatgact cgatttcgaa
     1741 gcgatcgacc gcgccgcgcc gcgcctgcat gcgttcgagg ccgaggcgca ccggcacatc
     1801 gagcaggatg gtgcagtcgg gattgagatc gccgtgcacc gcgtgcgcga tctggtcgat
     1861 gagcttgcga tcgacgccgc gcccgccgcc ctggtaagcg tgcgtggcgt ccgtgaaccg
     1921 gtcgcagagc acccattcgc ctcgcgtgag cgagggccgg atgaggttgt cgacgtgcac
     1981 ctggcgcgcg ccgaacatga gcagggtctc ggcggtggcg ctgatgtgtt cttcgccgcg
     2041 ctcgagcacg agcgcgcgca cgcgttcggc aaccttggtt ccaccgggtt cgcgcgtggc
     2101 atgcacggta aggccgcgcg cgcgcagcgc ctgcgtgagg cgatcggcga ccgtcgtctt
     2161 gccggcgccc tcgattcctt cgagcgtgat gaatttggcc ggcgtcatct gggtactctt
     2221 gctcctctca atcgcgcgcc cggaggcgtg caagataccg cgctaccgcg gcattgtgcg
     2281 cttcgagcgt agccgaaaag acatgtgatc cgtcgccgat tcccgtggcc acgaagaaga
     2341 tgtcgcccgt ctcgagcggt cgcgcgaccg cttcgatggc ttcccgcgac ggcagcgcga
     2401 tcggcgtcgg cggcagtccg cttcgcgtgt aggtgttgta cggcgtatcg gtgcgcaggt
     2461 cgcgttcgcg gatgttgccg tcgtagttgg cgccgagtcc gtagatgacg gtggggtcgg
     2521 tctgcagccg catgtttcgc cgaagacgcg tggtgaacac gccggcgatg cgcggccgtt
     2581 cgctggcgag gcccgtttcc ttttccacga tcgacgcgag cgtgagcgct tcgtacgcgt
     2641 tggcgagcgg cagcgcggcg gcacgcgcac tccacgcggt ttcgagggtc tccgccatct
     2701 tgcgataggc gagcgcgtag agatcgcgat cggaagttcc ggccgcgaat cgataggtgt
     2761 ccgggtagaa gcgcccttcg ggatgttcgc ccgcgtgacc gatggcggtc atgagcgcgg
     2821 cgatatcctt gccgcgcagc gtcacgttga tctgcgtatg cgcttcgacg gcgcgacgca
     2881 tgtccgcaaa cgtccagcct tcgatgaccg tgagcgattc cagcaccacc cgcccctcgg
     2941 cgagctggcg caggatttcc tgcggactcg cctgcgcggg aatttcgtag tgcccggtct
     3001 tgatctgggg agagccccga acgcgcaggt agatctccac ggcacgacgg tcggccaggg
     3061 cgccgcgagt ctcgagttcg gcgagcgccg cgcgtaccgt ggtgccgggt ttgaccatga
     3121 gaggcacgga atcgaggtgc gggccgtccg ctgtcatggc cgcgttggcg cgttgccagt
     3181 agatagccgc cgccacgccg gcgagcacga ccgcgagtcc aaggatccag agcagcttac
     3241 gcatccagcg gctcgatgtg cgcggcgagg cgcaatgcca gggtattcat gccgaacgaa
     3301 tgctctccca cgcgccgcac cggcacaacc ccgattcggg cattggtgat gaatacctca
     3361 tcggcggaaa acagatcctc gagcgacaag cgctcctccg aggcggcgat gccgagcaag
     3421 gcgcactcgc gaaggacgat gccgcgcatc acacccgaca ctccgcaacg gtcgatgcgc
     3481 ggtgtggcca cgcggccatc gcgcgcgatg aacacattgc tcatcgcgcc cgacaccagg
     3541 tgtcccgacg cgtcgagcag cagggattcg tagttatcgg gttcgcaggc ttccgccgcg
     3601 gccaacacgt tctcgagccg gttcaggtgt ttgattccgg caagcgacgg gttctcaccg
     3661 agccgcagcg tggccacgcg aagtttcacg cccgaccccc actcgcctgc agccgtcatg
     3721 ggccacagtg atacgagacg ccgcggcgtt tccgttccct gcggcgcata cccgcgccgc
     3781 gtcgcgctgc cgcgagtcac gatgatcttg aggatggcca aagacggcgc ctccgaactc
     3841 gcgagggcga tctccgcgcg aagctcggcc agcgccgcaa agggaatggc gaggcgtgcg
     3901 caaccgagcg tcagccgcgc aagatgcgat tcgaggaagc gcggacgtcc cgcgcgaacc
     3961 acgatggttt cgaaaatgcc gtcgccatat tgaagtccgc gatcatcggc cggcagcacc
     4021 tcgccgcgga ttccgtctat ccagcaaccg agcgcggcgc tcatcgcgtt tctccggccg
     4081 gtgtcgcaaa cgcgcgcaac atgccgcgcg ccttggctcg cgtttcatcg agttccttct
     4141 cgggaacgga gtcggcgacg attccggcgc cggcgcgcag ttcgatgacg tcgtcacgca
     4201 tggtcagcgt gcgaatgagg atattgaagt ccgcgtcgcc gtcggtgccg agccagccga
     4261 gcgaaccggt gtaggcgcca cgcccctcgc cttccagcgc cgcgatgatc tgcatgcaac
     4321 ggaacttcgg acacccggtg atggtgccgc cggggaacag cgcccgcagc gcgtccacgg
     4381 gcgagcagtc ggcgcgcaac cggccgcgca cgttggagac gatgtgatgc acgtgtgcat
     4441 aggactcggt caccatgtat tcgtccacgg tcacgcttcc ggcctcacag acgcggccga
     4501 ggtcgttgcg ttcgaggtcg atcaacatga tgtgttcggc gcgctccttg ggatgcgcca
     4561 ccagttcggc cgtatcacgc agatcctgtt cggccgagcc ctggcgcgcg cgggtaccgg
     4621 cgatgggcct cgtggaaatt tcacggccct cgatgcgcag caggcgttcc ggcgatgacg
     4681 agaaaatcgc catgccctgg acctgcacgc tggccgcgaa tggcgccggg ttggccgtgc
     4741 gaagtgcgtc gtacacgcgg gcggcgcttt cgggatgccg cagtttcaga cgccagcgac
     4801 gcgacaggtt cgcctggtag agatcccctg ccgcgatgtg ttcctgtgcg gcgcggacac
     4861 gcgcgaggaa aagctcggca gcttcttcat ccagcgcgct gagttccagt ctcgagcccg
     4921 ccgacgccgg ctggccgtgg gcggacgcga ggtcggccat gagctccgca tgcgttgcgg
     4981 aatcggcttt ctcggagatc gcatacacgc gatccgtgcc gaggtcgtga atcgcgaagg
     5041 tgtcgacacg caatgcgaat gcggaaaaag gactgtccgc cggcggcaac ttcagccgcg
     5101 gttcgatctc ggccgcgact tcgtaaccca ggtagacgaa ccagccgccc acgaacggca
     5161 aagctttggg cgtggccgaa ggtgttgccg gagtcgcttc gtcgcgaaac caggcttcga
     5221 gattgtcgag gaatccgccg gcgacaaggc ttcggccctg cgcgctcaag cgcccgctcg
     5281 cgtcgcgaaa caacgtggcg gaaggcttgc aggccagcat gctgaaacgc gccaggggcc
     5341 cgttcgcggc gctgtcgaag aacacgggat attgcgcagg ccgcgacgcg gcgagcgaac
     5401 gcagtgcttc gcccgacacc tgaacggcgg tgatgagccg ccccagggcg tcagcccttg
     5461 aagcggcgga agatcagcga gccgttggtg ccgccaaacc cgaaggagtt cgacagcgcg
     5521 gcttcgatgg cgagcttgcg cgccgtattc ggcgtgtagt cgagatcgca gtccggatcc
     5581 ggcgtgtgat agttagtcgt cggcggcgcg acgccgtcgc gaatggccag gatcgagaag
     5641 atggcttcca cgactcccgc cgcacccagc aggtggccgg tcatcgactt ggtcgagctg
     5701 atcgacatct tgtacgcgta gtcgccgaag gcgtgcttga tcgccacggt ttcggccttg
     5761 tcaccgagcg gtgtcgaggt cgcatgggcg ttcaggtact ggatgtccgt gggattgagt
     5821 tgcgcatcgc gcagcgcatt caccatggcg agacgagcac ccgcgccgtc ttccggcggc
     5881 gccgtgatgt gataggcgtc gccgctcatg ccgaagccga tgcactcggc gtagatgcgc
     5941 gcgccgcgcg ctttcgcatg ttcgagttct tcgaggatga cggcgccgcc gccatcgccc
     6001 atgacgaagc catcgcggtc cttgtcccag ggacggctcg cgccctcggg atcgtcgtta
     6061 cgctcggaca gtgccttcgc ctggctgaag cccgccagtg ccgtcggcgt ggtggcgcac
     6121 tcggatccgc ccgcgatgat gatgtccgca tcgccgtact ggatgcagcg catcgcaagg
     6181 ccgatggcat gcgtggacgt cgtgcaggcg ctgaccacgc ccaggttcgg accgcgcagc
     6241 ccgaaacgga tcgacaggtg gccagccacc atgttgccga tggtgctcgg gatgtagaac
     6301 ggcgaagtct tcttcgggtt gtgggtgcgt tcccaggtga tcgcttcgtc ttcgatgccc
     6361 gcgaggccgc cgatgcccga acccatgatg gcgccacaac gatcgccatt ttccttggcg
     6421 aaatcgagac ccgaatccgc cgccgcctga attcctgcgc ccatgccgta gtgcatgaac
     6481 gggtccatgc gccgcgcttc cttgggcgag atgtaggtgg caacgtcgaa ccccgacact
     6541 tcgcccgcga acttcgtggc gaacggagtg acgtcgattc gcgtgatcgg cttgatgccg
     6601 ctcttgcccg cgagaatgga cgaccacgca gtggtgacgt cactgcctac cggcgaaacg
     6661 ataccgagcc cggtaacgac gacacgtcgc ttggacacac tatcctctgt gtattgaagg
     6721 cggatggatg gcggcactgg cgcggctcat gagctgcgca ccaggccgcg aaacaaatct
     6781 caggacttgc ggcgatcaga gatgtacgtg atcgcctggc tcacggtcgt gatcttctcg
     6841 gcatcttcgt cggggatctc gatttcgaac tcttcttcga gagccatgac cagctcgacc
     6901 gtatcgagcg aatcggcgcc gaggtcatcg acgaacgaag cgtcattcac aacctgctca
     6961 gccttgacgc ccaactgttc ggcgacaatc gccttgacct gctgttcaac cgtgctcatt
     7021 tctgattcgc cctcaaagtg tgtgtagtcg ccgcaacccc cg
//
LOCUS       DATDEU010000260         3839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_484427, whole genome shotgun sequence.
ACCESSION   DATDEU010000260 DATDEU010000000
VERSION     DATDEU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484427"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VIV63_06305"
     CDS             complement(<1..1118)
                     /locus_tag="VIV63_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6124243.1"
                     /translation="MSLFDRCDFDFGLGPELDEVRNQVRRFARERIAPRAEEIDRSNE
                     FPRDLWPELGSAGLLGITVQERWGGATLGYLAHIICMEEISRASGSVGLSYGAHSNLC
                     MNQIMLNGNDAQRDRYLPPLVEGVHVGALAMSEPGAGSDVVSMSLRAEKRGDAYALTG
                     SKMWITNGPDADVVVVYAKTDPAAGARGITAFIVEKGYAGFTTAQKLDKLGMRGSSTS
                     ELVFDNCLVPAENMLGQLNGGVRVLMKGLDYERAVLGGGPLGLMAAALDLVLPYIHER
                     KQFGQPVGTFELMQAKVADMYASLNAARAYSYAIGRACDAGHCSRQDAAAAILFGADH
                     ATQNCLQAIQALGGNGYINDYAAGRLLRDAKLYEIGAGT"
     gene            1176..2789
                     /locus_tag="VIV63_06310"
     CDS             1176..2789
                     /locus_tag="VIV63_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY6124244.1"
                     /translation="MPLLDSKVDRKSAEYAANQAAMQALVTELRSRQARIGAGGGEAA
                     RAKHVARGKLLPRDRVEMLLDPGAPFLELSPLAAFGMYGDESPGAGIITGIGRIAGRE
                     CVVVCNDPTVKGGTYYPVSIKKHLRAQRIAEENRLPCVYLVDSGGAHLPSQDEVFPDA
                     EHFGRIFYNQANMSAAAIPQIAVVMGSCTAGGAYVPAMSDESIIVRNQGTIFLGGPPL
                     VKAATGEVVTAEELGGGDVHARISGVVDHLAENDGHALGIARRIIGGLPADPLSNAPM
                     PAAAFEEPKYPAEDLYGLIPADTRKPYDAREIIARLVDASDFDEWKARFGVTLVTGFA
                     RIHGMPVGILANNGILFSDSAQKGAHFIELCCQRRVPLVFLQNITGFMVGKKYENEGI
                     ARHGAKMVTAVACARVPKFTVIVGGSFGAGNYGMCGRAYAPRFLWTWPNSRISVMGGE
                     QAANVLATVRRDAIEAKGGAWSGEEEAAFKAPIREQYETQGNPYYATARLWDDGVIDP
                     ADTRMCLALGLGVATRQPIPETRFGVFRM"
     gene            2803..3597
                     /locus_tag="VIV63_06315"
     CDS             2803..3597
                     /locus_tag="VIV63_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY6124245.1"
                     /translation="MTSYQHIEIEQRGAVRWLWLNRPEVRNAFNDVLVAEIAAAFADV
                     EASTDTRVVVVGARGPAFCAGADLNWMRSMASFSHADNHADALRVARMFAAVHACSRP
                     VIARVQGDAFGGGVGLVAACDVVVAVEAASFVLSEVKLGIVAATISPHLVRAMGARAA
                     SRYMLTAEKFSAAQARDLGLVHEVTLSEGLDREVERLVHHLLAASPAALAATKRLLAD
                     VVEAPLDDVLLAATAKCIADARVSPEGREGIAAFLEKRPPPWSPKK"
     gene            3604..>3839
                     /locus_tag="VIV63_06320"
     CDS             3604..>3839
                     /locus_tag="VIV63_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY6124246.1"
                     /translation="MFAKLLIANRGEIACRIARTARRMGVRTVAVYSDADARAAHVAA
                     CDEAVHIGGPRPQESYLRGDVILEAARRTGADAI"
BASE COUNT          630 a         1243 c         1305 g          661 t
ORIGIN      
        1 gtgccggcgc cgatttcata gagcttggcg tcgcgcaaca gtcttccggc cgcgtagtcg
       61 ttgatgtagc cattaccgcc cagtgcctgg atggcctgca ggcaattctg cgtggcgtga
      121 tcggcgccaa acaggatggc cgcggcggcg tcctggcggg aacagtgccc ggcgtcgcag
      181 gctcggccga tcgcgtacga ataggcgcgc gcggcgttca gcgacgcgta catgtcggcc
      241 actttcgcct gcatgagttc gaacgtgccc acgggctggc cgaattgttt gcgttcgtgg
      301 atgtagggca acaccaggtc gagtgccgcg gccatgagac cgagaggccc gccgccgagc
      361 acggcgcgtt cgtagtcgag gcccttcatg agcacgcgca cgccgccgtt caactggccc
      421 agcatgtttt ccgcgggcac gaggcaattg tcgaacacga gttccgacgt ggacgagccg
      481 cgcatgccca gcttgtcgag cttctgcgcg gtggtgaaac cggcgtagcc cttttccacg
      541 atgaaggccg tgatgccgcg cgcacccgcg gcgggatccg tcttggcgta aaccacgacc
      601 acgtcggcgt cggggccgtt ggtgatccac atcttgctgc cggtcaacgc gtacgcgtca
      661 ccgcgttttt cagcccgtag cgacatgctc acgacgtccg agccggcgcc gggttcgctc
      721 atggcaagcg cgccgacgtg cacgccttcc accagcggcg gtagatagcg gtcgcgttgc
      781 gcgtcgttgc cgttgagcat gatctggttc atgcacaggt tggagtgcgc accataggag
      841 aggcccaccg agccggaggc gcgcgagatc tcctccatgc agatgatgtg cgcgaggtaa
      901 ccgagggtgg cgccgcccca acgctcctgg acggtgatgc ctaacaagcc ggcggacccg
      961 agctccggcc acaggtcgcg cgggaattcg ttgctgcggt cgatttcctc ggcgcgcggc
     1021 gcgatccgct cacgggcgaa tcgccgcacc tggttgcgga cctcgtcgag ctcaggcccc
     1081 aggccgaagt cgaaatcgca gcggtcaaag aggctcatgt ttttgactca cttggcttgc
     1141 cttcaaatgt cgggttgtac tattgtccga caatcatgcc gctgctcgat tccaaagtcg
     1201 ataggaaaag cgctgagtac gccgccaacc aggcggcgat gcaggcgctg gttaccgaat
     1261 tgcgcagccg gcaggcccgg atcggcgcgg gcggaggcga ggcggcgcgc gccaaacacg
     1321 tggcgcgtgg aaaattgctg ccccgcgatc gcgtggagat gctgctcgat cccggcgccc
     1381 cgttcctcga gttgtcgccg ctcgcggcct tcggcatgta cggcgacgaa tcgcccggcg
     1441 cgggaatcat caccggcatc ggccgcatcg ccggccgcga atgcgtggtg gtgtgcaacg
     1501 acccgacggt gaagggcggc acgtactatc ccgtcagcat caagaagcac ctgcgcgcgc
     1561 agcgcatcgc cgaagaaaac cggctcccct gcgtctacct cgtggactcc ggtggcgcgc
     1621 atctgcccag ccaggacgaa gtctttcccg acgccgagca tttcggccgc atcttctaca
     1681 accaggccaa catgtcggcc gcggccattc cgcagatcgc ggtggtcatg ggttcgtgca
     1741 cggcgggcgg cgcctatgtg ccggcgatga gcgatgaatc gatcatcgtg cgcaaccagg
     1801 gcaccatctt tctcggcggg ccgccgctgg tgaaagccgc cacgggcgaa gtggtgacgg
     1861 ccgaagaact gggtggcggc gacgtacacg ccaggatctc gggtgtcgtc gatcacctcg
     1921 ccgagaacga cggtcatgcg ctcggaatcg cgcggcggat catcggcgga ctgccggcgg
     1981 atcctctctc gaatgcgccg atgccggccg cggcattcga ggaaccgaag tatccggccg
     2041 aagatctgta cgggctcatt cccgccgaca cgcgcaagcc gtacgacgcg cgcgagatca
     2101 tcgcgcgcct cgtcgacgcc agcgacttcg atgaatggaa ggcgcgattc ggcgtcacgc
     2161 tggtcacggg attcgcgcgt atccacggca tgcccgtcgg catcctcgcc aacaacggca
     2221 tcctgttctc cgattccgcg cagaagggcg cccatttcat cgagctgtgt tgccagcggc
     2281 gcgtgccgct ggtcttcctg cagaacatca ccggcttcat ggtcggcaag aagtacgaga
     2341 acgaaggcat cgcgcgccac ggcgcgaaga tggtcacggc cgtcgcctgc gcgcgcgtgc
     2401 cgaagttcac ggtcatcgtc ggtgggagtt tcggcgccgg aaattacggc atgtgtggac
     2461 gggcctatgc gccgcgcttc ctgtggacct ggccgaactc gcgcatcagc gtcatgggcg
     2521 gcgagcaggc ggcgaatgtg ctggcaaccg tgcgccgtga cgccatcgag gcgaagggtg
     2581 gagcctggag cggcgaagaa gaggccgcgt tcaaggcgcc gattcgcgag cagtacgaga
     2641 cgcagggcaa tccctattac gccacggcgc gcctgtggga cgatggcgtc atcgaccccg
     2701 ccgatacgcg catgtgcctc gcgctgggcc tgggcgttgc cacgcggcag ccgatccccg
     2761 agacgcgctt cggcgttttc cgaatgtgag cggatgtgat gcatgactag ctaccagcac
     2821 atcgagatcg aacagcgcgg ggcggtgcgc tggttgtggc tcaaccgccc cgaagtgcgc
     2881 aatgcgttca acgacgtgct ggttgctgag atcgccgccg cgttcgcgga tgtcgaggca
     2941 tcgaccgaca cccgcgtggt ggtcgtcggc gcgcgcggtc cggccttttg cgctggagcc
     3001 gatctcaact ggatgcgctc gatggcttcc ttcagccacg ccgataacca cgccgatgcc
     3061 ctcagggtgg cgcgcatgtt cgccgcggtg cacgcgtgtt cgcgtccggt catcgcccgc
     3121 gtgcaaggcg acgcgtttgg tggtggcgtc gggctcgtgg ccgcctgcga tgtcgtggtc
     3181 gcggtagaag ccgcgagctt cgtgttgtcc gaggtcaagc tgggcatcgt cgcggcgacc
     3241 atctcgccac acctcgttcg cgccatgggc gcgcgcgccg cctcgcggta catgctcacc
     3301 gccgaaaaat tctccgccgc ccaggcacgc gacctggggc tggtgcacga ggtcacgttg
     3361 tcggaaggtc tggatcgcga agtcgagcgc ctggtgcatc acctgctggc ggcaagcccc
     3421 gcggcactcg ccgcgaccaa gcggttgctc gcggacgtgg tcgaagcgcc gctggacgac
     3481 gttctgctgg cggcgaccgc aaaatgtatt gccgatgcgc gagtctcgcc tgaaggccgt
     3541 gaaggcatcg cggcgtttct cgagaagcgc ccgccgccct ggtcgccgaa gaagtagctt
     3601 ctcgtgttcg ccaaactgct gatcgcgaac cggggagaga ttgcctgccg cattgcgcgc
     3661 acggcgcggc gcatgggtgt ccggaccgtg gcggtctact cggatgcaga cgcgcgagcc
     3721 gcgcatgtcg ccgcctgcga tgaagccgtc cacatcggcg gcccgcggcc ccaagagagt
     3781 tacctgcgcg gcgatgtcat tctcgaggcc gcgcgtcgca cgggcgcgga tgccatcca
//
LOCUS       DATDEU010000261         6591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_192007, whole genome shotgun sequence.
ACCESSION   DATDEU010000261 DATDEU010000000
VERSION     DATDEU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192007"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1920)
                     /locus_tag="VIV63_06325"
     CDS             complement(73..1920)
                     /locus_tag="VIV63_06325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6124247.1"
                     /translation="MTGRHTRRWFASIPITGRFYIMLAILVLAEAGLVAGCLRAMELQ
                     TDASADLARVAAVQRSLDRALTLHTNIGTELQSAATMPSKTPNNLVGALRAQIDLTWA
                     LPASAEVTAITDSLKVPAAQYLESVEEYVRQGGGAGSATAWAFADLEPRRVGLETALQ
                     DSMTRMRGVLQRVESRVLAEAGRAGTVKAWLIGGALAAAIVAMLVFWAIAKSIQNALR
                     DVGEVAQALAAGDMDNRCDTHGSDEVSGLARMINLLADTMQGMLTRLRSEAGRSTFSN
                     QLVEALEMADSEAEADRVIARAMSAISDVHPMELLLADSSRAHLERACVHPKNGSPNC
                     SVESPYSCIAVRRGHPQIFPDAEALNACPKLLGRMGGSLSAACVPVNFMGRALGVLHV
                     AGAKGKPPNPEQIAQLTALGVHAGTRIGTLRAFERSQLQASTDSLTGLNNRRAAEEVL
                     SNLMAVDSAFAVVMADLDYFKRLNDTQGHESGDRALKLFADTARGVVRDRDHVVRWGG
                     EEFALLLPGANAERAAEVVGRLRAALAEAHLLSGTPVFTASFGISDSTMTRDRETLIR
                     LADEALYRSKQAGRDCCTVGDPQVLSIPGVRRERDQGAVVDITQVARSS"
     gene            complement(2059..5208)
                     /gene="putA"
                     /locus_tag="VIV63_06330"
     CDS             complement(2059..5208)
                     /gene="putA"
                     /locus_tag="VIV63_06330"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEY6124248.1"
                     /translation="MTKFPPLERPDSIAKAINPQYLTEEQPLVRALADQARLTEAAGR
                     NAGARALALVQAVRAARGSGGALDAFLREYSLASREGVILMCLAEALLRIPDGETADR
                     LIADKIPSGAWDEHLGDSDSMLVNASTWGLMLTGRVVALDRNEVGEARSWYARLVGKL
                     GEPVARAALKQAMRILGHQFVMGRDIESALTRAAGKEERDYRYSFDMLGEAALTRADA
                     ERYREKYSAAIAAVGKAVETRESITARHSISIKLSALHPRYEFAHAPRVMAELYPVIE
                     TLVREARDAGIGVTLDAEEAERLELSLLLVNKLLDSDVTRGFAGFGLAVQAYQKRAYA
                     TIEWLMQRLRETDRRITLRLVKGAYWDSEIKRAQERGLAGYPVFTRKPNTDVSYLACA
                     RLLESAGERVYPQFATHNAHTIAHVAEVFGGDTNRFEFQRLHGMGAELYDSVVRGPWG
                     KYPCRVYAPVGAHEDLLPYLVRRLLENGANTSFVNRIVDASLPAEEVVADPVAEVDAL
                     ERVAHPRIPLPVNLFAPERANSAGFNFADGQAVDGLMRDMDRASQQPWSAAPLLSGKS
                     QTGKTSALFNPADTAEQIGSVTSADAATVDGAIAAALRAQEDWDDFGGEKRAAILERA
                     ATLFEEHTAAFVARCVREAGKTLPDAIGEVREAVDFLRYYAARARRDFSHESALPGPT
                     GERNLLRLRGKGVFGCISPWNFPLAIYTGQVAAALAAGNTVVAKPAEQTPLTAAYATG
                     LLLQAGVPPEALQFVPGDGAEVGGALTRDPRLAGVVFTGSTDTARLIERSMAARPGAI
                     GTLIAETGGLNVMLADSSALAEQLVLDTVHSGFNSAGQRCSALRVLLVQEEIAPRVKT
                     LLAGCMDDLILGDPARLGTDVGPVIDDDALAMLEKHAAQIVKGSKWSHRARPATHTRG
                     RFFAPLAVEIESLSSLQREVFGPVVHVVSYRARDLDAVVGAINALGYGLTLGIHTRID
                     GLAQRIARRLRVGNVYINRNMIGAVVGVQPFGGMGLSGTGPKAGGPHYLHRMATEQTI
                     TTNTAAIGGNAGLLSLAAR"
     gene            5256..6074
                     /gene="trxA"
                     /locus_tag="VIV63_06335"
     CDS             5256..6074
                     /gene="trxA"
                     /locus_tag="VIV63_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY6124249.1"
                     /translation="MSASVSAVTTATFPAEVVGASASQPVLVDFWAPWCGPCRALGPV
                     LEQLADENVGKIKVVKVNTDENQDLAQQFQIRGIPAVKLFRNGRVVDEFVGAQPLSQV
                     RAFLEPHLPRASAAEHIAAQEHAGRGDFAGAVTQLRKIAGTDPANLDARRDLSRYLAL
                     SGEVVEASKVLGQLPPQVQNEPATNALRALIHFAALATEEAARTDSLRAGAARSLLGG
                     SPDAAIESLLTRMQGDRVFATRAGREDLLQAFALLPADDARVTGWRRRLAALLN"
     gene            6074..6322
                     /locus_tag="VIV63_06340"
     CDS             6074..6322
                     /locus_tag="VIV63_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124250.1"
                     /translation="MNDDITTAVKVLAVLGGATVLLWIGVVMVKAAKRGGGGMQALGA
                     ALMMLGWGNMRDPSNNTVAEAQDGRIRKGTHSGDPLEK"
     gene            6365..>6591
                     /locus_tag="VIV63_06345"
     CDS             6365..>6591
                     /locus_tag="VIV63_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124251.1"
                     /translation="MKTRPVPRAASRRQFVGRLALLAGAAPFLTGRAADQKSTRVPRI
                     GYLGGDFPEHRDAFMTELRTQGFVDGQNVAL"
BASE COUNT         1107 a         2232 c         2145 g         1107 t
ORIGIN      
        1 tccccgagga agttcttcga cttcagctga atggggacac tccccatttg ctggcaaaag
       61 gggccgcacc tgctagctgc tgcgcgccac ctgcgtgata tcgacgacag caccctgatc
      121 gcgctcgcgt cgcacgcccg ggatgctcaa cacctgcgga tctccgacag tgcagcagtc
      181 gcggcctgcc tgcttcgaac ggtacagcgc ttcgtccgcc aggcgaatca gcgtttcccg
      241 gtcgcgtgtc atcgtcgaat cggagatgcc gaagctcgcc gtgaacaccg gcgtcccgga
      301 cagcaagtgc gcttcggcaa gggcggcacg caaccggccg acgacttccg cggcacgctc
      361 ggcgttcgcg ccgggtagca gcaacgcgaa ttcctcgcct ccccagcgca ccacgtggtc
      421 gcgatcgcgg accacgcctc gcgcggtatc cgcgaacagc ttgagcgcgc ggtcgccgct
      481 ctcatgaccc tgcgtgtcgt tgaggcgctt gaagtagtcg agatcggcca tgacgacggc
      541 gaaggccgag tccaccgcca tgagattgct gagcacttct tcggcggcgc gccgattgtt
      601 gagtccggtc aggctatcgg tgctcgcctg caactgcgaa cgctcgaacg cgcgcagcgt
      661 tccaatgcgc gtgccggcat gtacgccgag cgcggtgagc tgcgcgatct gttcgggatt
      721 cggcggcttc cccttggcgc ccgcgacgtg caacacgccg agcgcgcggc ccatgaagtt
      781 cacgggaacg caggccgcgg acaacgaacc acccatgcgg cccaacaact tcggacacgc
      841 gttgagcgct tcggcatcgg gaaaaatctg cggatggccg cgacggaccg cgatgcagct
      901 gtacggcgat tccaccgagc aattgggcga accgtttttg ggatgcacgc aggcgcgttc
      961 gagatgcgca cgcgaggagt cggccagcaa cagttccatc gggtgcacgt cggagatcgc
     1021 actcatggcg cgcgcgatca cgcggtcggc ctccgcctcg gagtcggcca tctcgagcgc
     1081 ttccacgagc tggttgctga acgtgctgcg gcccgcctcg gatcgcaggc gcgtcaacat
     1141 tccctgcatg gtgtcggcga gcaggttgat catgcgtgca agaccgctga cctcgtcgga
     1201 tccatgcgtg tcgcagcgat tgtccatgtc gccggcggcc agcgcctgcg ccacttcgcc
     1261 cacgtcgcgc agcgcattct gaatggattt cgcgatggcc cagaaaacca gcatcgcgac
     1321 gatcgcggcc gcgagcgcgc caccgatcag ccacgccttc acggttccgg cgcgccctgc
     1381 ttcggcgagc acgcgcgatt cgacacgctg aaggactccg cgcatgcgcg tcatcgaatc
     1441 ttgcagcgcg gtttcgagcc cgacacgccg gggttcgaga tccgcaaacg cccatgccgt
     1501 cgcgctaccc gcgccgccgc cctggcgcac gtactcttcg acgctttcga gatattgcgc
     1561 ggccggcacc ttgagtgaat cggtgatcgc ggtcacttcg gcgctggccg gcagtgccca
     1621 ggtgagatcg atctgcgcgc gcaacgcgcc gaccaggttg ttaggcgtct tgctcggcat
     1681 cgtcgcggcg gattgcagct ccgtgccgat gttggtgtgc aacgtgagtg cgcggtcgag
     1741 cgagcgctga acggccgcga cccgcgcgag atccgcgctc gcatcggtct gcaattccat
     1801 cgcacgcagg cagccggcaa ccaggcccgc ttcggcgagc acgagaatcg cgagcattat
     1861 gtagaaacgc ccggtgatgg gaatggacgc aaaccaccgg cgcgtgtgtc ttccagtcat
     1921 tgcagtacgc ccctccggcg attcgcacag aagggtatcg gctgcgccgg ggtggacctt
     1981 agggcggcaa aacgcaaacc gccccgcccc gcgcgacgca gtgctcagat tccaccgtac
     2041 agggcgctgt ctggcgtgtt accgggccgc cagcgacaac aagccggcgt tgcctccgat
     2101 ggccgcggta ttggtggtga tcgtctgctc ggtagccatg cgatgcaggt agtgcggacc
     2161 acccgccttc gggcccgtgc ccgaaaggcc catgccgccg aatggctgca cgccgaccac
     2221 tgcgccgatc atgttgcgat tgatgtagac gttgccgacg cgtagccgtc gcgcgatgcg
     2281 ttgcgccagg ccgtcgatac gcgtgtggat gccgagcgtc agtccgtagc cgagtgcgtt
     2341 gatcgcgccg accaccgcgt cgagatcacg cgcgcgataa ctgacgacgt gcaccaccgg
     2401 accgaacact tcgcgctgca acgacgacag cgattcgatc tccacggcca gcggcgcgaa
     2461 aaaccgtccg cgcgtgtggg tggccggccg cgcgcgatgg ctccatttcg aacccttgac
     2521 gatctgcgcg gcgtgtttct cgagcatcgc gagcgcatcg tcgtcgatca ccggaccgac
     2581 atcggtaccg agtcgcgccg gatcgccgag tatgagatcg tccatgcagc cggccagcaa
     2641 cgtcttcaca cgaggagcga tctcctcctg caccagcagc acccgcagcg ccgagcatcg
     2701 ctgccccgcg ctgttgaatc ccgagtgcac ggtgtcgagc acgagttgtt cggcgagcgc
     2761 cgaactgtcg gcgagcatca cgttgaggcc gccggtctcg gcgatcagcg taccgatggc
     2821 gccgggccgc gcggccatcg atcgctcgat gagacgcgcg gtgtcggtgg atcccgtgaa
     2881 caccacgccc gccagccgcg ggtcgcgggt cagcgcgcca cccacttcgg cgccatcgcc
     2941 cggtacgaac tgcagtgctt cgggcggaac gccggcctgc agcagcaacc cggtggcgta
     3001 ggccgcggtc agcggagtct gctcggcggg cttggcgacg acggtgttgc cggccgcgag
     3061 cgccgcggcc acctggcccg tgtagatagc cagcgggaaa ttccagggac tgatgcaacc
     3121 gaacacgccc ttgccgcgca ggcgcagcag gttgcgctcg cctgtcgggc ccggcagcgc
     3181 agattcgtgc gagaaatcgc gacgcgcacg cgccgcgtag taacgcagga aatccaccgc
     3241 ttcacgtact tcgccgatgg cgtcgggcaa cgtcttgccg gcttcgcgca cgcagcgcgc
     3301 cacgaaggcg gcggtgtgct cctcgaacaa tgtcgcggcg cgctcgagaa tcgcggcgcg
     3361 tttttcgccg ccaaaatcat cccaatcttc ctgcgctcgc agcgctgcgg cgatggcgcc
     3421 gtcgacagtg gcggcatctg cagacgtcac gctgccgatc tgctcggcgg tatccgcggg
     3481 attgaacagc gcactcgttt tgcccgtttg cgacttgccg gacaggaggg gcgccgcgct
     3541 ccagggttgt tgggaggcgc gatccatgtc gcgcatcagg ccatccaccg cctggccatc
     3601 ggcaaaattg aagcccgccg aattggcgcg ttcgggcgcg aacaggttca cgggcagcgg
     3661 aatgcgcgga tgcgccaccc gctccagtgc gtcgacttcg gccaccggat ccgccaccac
     3721 ctcctcagcg ggcaggctcg catcgacgat gcgattgacg aacgacgtgt tggcgccgtt
     3781 ctcgagaaga cgccgcacga ggtacggcaa caggtcttca tgcgcgccga cgggtgcgta
     3841 gacgcgacat ggatatttgc cccagggccc gcgcaccacg ctgtcgtaaa gctccgcgcc
     3901 catgccatgc aggcgctgga actcgaagcg attggtgtca ccgccaaaca cctcggccac
     3961 gtgcgcgatg gtgtgcgcat tgtgtgtggc gaactgcgga tacacgcgct cgccggcact
     4021 ttcgagcagg cgtgcgcagg caaggtagga gacgtcggtg ttcggtttgc gcgtgaacac
     4081 cgggtagccc gccagcccgc gctcctgcgc gcgcttgatc tcgctgtccc agtacgcacc
     4141 cttgacgaga cgcaacgtga tgcgccggtc ggtctcgcgc agccgttgca tgagccattc
     4201 gatcgtggcg taggcgcgct tctgatacgc ctgcaccgcg agtccgaaac ccgcaaagcc
     4261 tcgcgtcacg tcgctgtcga gcagcttgtt gaccagcagc agtgacagct ccaggcgttc
     4321 ggcctcttcc gcgtcgagcg tcactccgat gccggcgtcg cgcgcctcgc gcaccagggt
     4381 ctcgatgacg ggataaagct ccgccatgac tcgcggcgcg tgggcgaatt cataacgcgg
     4441 atgcagcgcg gacaacttga tggagatgct gtggcgcgcg gtgatggact cgcgcgtttc
     4501 cacggccttg cccacggccg cgatggcggc cgaatatttt tcgcgataac gctccgcatc
     4561 ggcgcgcgtc agcgcagcct cgccgagcat gtcgaacgaa taacgataat cgcgttcttc
     4621 ttttcccgcg gcgcgcgtca acgcgctttc gatatcgcgc cccatgacga actggtggcc
     4681 gagaatgcgc atggcctgtt tgagcgcggc gcgtgccacc ggctcgccga gtttgcccac
     4741 gagccgtgca taccaggacc gggcttcgcc cacctcgttg cggtcgagcg cgaccacgcg
     4801 gccggtgagc atgagacccc acgtggacgc gttcaccagc atcgagtcgg agtcgcccaa
     4861 gtgttcgtcc cacgcgccgc ttggaatctt gtcggcgatg agccggtccg cggtctcgcc
     4921 gtcgggaata cgcagcagcg cttcggcgag gcacatgagg atgacgccct cgcgcgaagc
     4981 caagctgtac tcgcgcagga aggcgtccag cgcgccgcca ctgccgcgcg cggcgcgtac
     5041 ggcctgcacc agggccagcg cccgcgcacc ggcgttgcgg cccgcagctt cggtcaggcg
     5101 cgcctgatcc gcgagggctc gtacaagtgg ttgttcttcg gtgagatatt gcggattgat
     5161 cgctttggcg atggagtccg gccgttcgag cgggggaaac tttgtcatgg cgaaattcta
     5221 ttgaattagc cggcgggcgt cccaatagcc gggccatgag tgcatcagta tccgccgtca
     5281 ccacggccac cttccccgcc gaggtcgtgg gcgcctccgc ttcccaaccg gttctggtcg
     5341 acttctgggc tccatggtgc gggccctgtc gcgcactcgg accggtgctc gaacaactgg
     5401 ctgacgagaa cgtcggcaag atcaaagtgg tcaaggtcaa caccgacgag aaccaggacc
     5461 tcgcacagca gttccagatt cgcggcattc cagccgtcaa actgttccgc aatggacgcg
     5521 tcgtcgatga attcgtcggc gcccagccgc tctcgcaggt gcgcgccttc ctcgagccgc
     5581 atctgccgcg cgcgtcggcc gccgaacaca tcgccgcgca ggaacatgcc ggtcgcggcg
     5641 acttcgcggg cgccgtgacg caattgcgca agattgccgg gacggatccc gccaatctcg
     5701 atgcgcgccg cgacctctcg cgatatctcg cgctctcggg cgaagtcgtc gaggcctcga
     5761 aggtgctcgg ccaattgccg ccgcaggttc agaacgaacc cgctaccaat gcacttcgcg
     5821 cgctcatcca cttcgcggcg ctcgccaccg aagaagcggc gcgcacggac agcctgcgtg
     5881 ccggcgccgc gcgttcgctg ctcggcggaa gtcccgatgc cgccatcgaa tcgctgctca
     5941 cacgcatgca aggcgatcgt gtgttcgcca cgcgcgccgg acgcgaagac ctgctgcagg
     6001 cgttcgcgct gttgcccgcc gatgacgcgc gcgtgaccgg ctggcgccgg cggctcgcgg
     6061 ccttgctcaa ctgatgaacg atgacatcac cacggcggta aaagtgctgg ccgttctcgg
     6121 tggcgccacg gtgctcctct ggatcggcgt cgtcatggtg aaggccgcga agcgcggcgg
     6181 cggcggcatg caggcgttgg gcgccgcact catgatgctc ggctggggaa acatgcgcga
     6241 tccaagcaac aacacggtgg ccgaggcgca ggacggccgc atccgcaagg gcacgcactc
     6301 cggcgatccg ctcgaaaaat aggcttccat ggtttttccc gcgtagagag cggaagctcg
     6361 cctaatgaaa accaggccag ttccacgcgc ggcgtctcgc cgccagtttg tcggaaggct
     6421 cgcgctgctc gcgggcgccg cgccgttcct caccggtcgc gcggcagatc aaaagtccac
     6481 gcgcgtaccg cgcatcggct atctcggcgg cgattttccc gaacaccgcg atgcgttcat
     6541 gacggaattg cgcacgcagg ggttcgtcga cggccaaaac gtggctctcg a
//
LOCUS       DATDEU010000262         5217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_46415, whole genome shotgun sequence.
ACCESSION   DATDEU010000262 DATDEU010000000
VERSION     DATDEU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46415"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VIV63_06350"
     CDS             <1..869
                     /locus_tag="VIV63_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124252.1"
                     /translation="PGERARASDVNGNFNNLKIAVEAAERRNTGLQNRIRDLEAALSN
                     VRTLDAVLTVETINGVRTVRLTGVNLQVVNGLSRTETINGAGNVIIGYDEANSFTTRP
                     ICSQASALNGTAITDEAGCLAAGGVFSTQHKSGSHNLVLGTQNGYSSYGGIVGGRFNF
                     INEIFATVVGGVDNRASGRFSAIVAGQGNLTQDSAAILGGNGNQALARNSSITGGIGN
                     VASGDNASITGGERNTVSGRASTVSGGLLNTASGSGANVSGGRDNVASGTHSSILGGS
                     GRTLAGANASQP"
     gene            879..1043
                     /locus_tag="VIV63_06355"
     CDS             879..1043
                     /locus_tag="VIV63_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124253.1"
                     /translation="MRTFARLAITLLAASLLGACGGSGEGAAPAGDSPAVAESHAWDS
                     WNWDEGSFGD"
     gene            1091..1435
                     /locus_tag="VIV63_06360"
     CDS             1091..1435
                     /locus_tag="VIV63_06360"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY6124254.1"
                     /translation="MNISTFAALADPMRLALIEALRDGEQPVNELVERVRIQQSGVSR
                     HLGILSRAGLVSVRPEGQQRFYSLRQQPFDELDEWIARIRAHWEARFDRFGAELERRK
                     AADTTKKKRSTK"
     gene            1432..1917
                     /locus_tag="VIV63_06365"
     CDS             1432..1917
                     /locus_tag="VIV63_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY6124255.1"
                     /translation="MKKRINLQRTFNAPLDLVWELWTTKEGIESWWGPPGFEVKVSKI
                     ELRAGGALEYVMTCVGADQIAFMKQHNQPLTTPLKARYTEVSPKTLAAWMNLADFIPG
                     VDPYEVETRLLLTALGADSVRVDLQLDAMHNEFYTSMATQGWESEMGKLAAVIDSRRK
                     S"
     gene            1914..2273
                     /locus_tag="VIV63_06370"
     CDS             1914..2273
                     /locus_tag="VIV63_06370"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6124256.1"
                     /translation="MKALIPFLWFADQAEEAARYYVGIFPGAKMGEIRRFPANSRGPE
                     GAVMTASFTLGDQTLVALNGNPRFTFSPAMSLLYPCASSDEAAALTEKLAEGGEKQPG
                     GWLRDKYGVSWQVHAIS"
     gene            complement(2270..3430)
                     /locus_tag="VIV63_06375"
     CDS             complement(2270..3430)
                     /locus_tag="VIV63_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY6124257.1"
                     /translation="MESDPAVRSRPKILFVDDEPRVLEGLVVLLRKDYEVHVATSGEQ
                     ALQKMRELQQLAVVISDMRMPQMDGATFLQAAMQRCPHAIRMLLSGEAGRDAAILAVN
                     KGQIFRFLSKPCPIEDLKTALEAAVAQHRLLNAERAVLQETLIGCIGALMEVLALANP
                     AAFGRANHIKRLACELAGRIGCTDFWQLEAAALLSQVGYLSLPPEVVEKVQQGVPLTP
                     DEQTLANGVPAVAIKLLDHIPRLDPVLQILTALTWTDAQITRLGEGTIGLGTRILGLT
                     LEFDALQAQGKSKDAALQSLSFRVGRFGAKLFGQLGHCPATDDSSQDSLEIPLRRVVP
                     GMTVLQELRTRMGILLAPKGFEVTRSFVERISNLAPELLDVNIRVAPKARAS"
     gene            3681..3824
                     /locus_tag="VIV63_06380"
     CDS             3681..3824
                     /locus_tag="VIV63_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124258.1"
                     /translation="MCAQAIDATEMELEVEFAEDCAHGVRDFHLHLPCFAAWEIERKV
                     APE"
     gene            complement(3843..4295)
                     /locus_tag="VIV63_06385"
     CDS             complement(3843..4295)
                     /locus_tag="VIV63_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124259.1"
                     /translation="MHISRTTAIHEAAHAVAAIRAGLVFDHVTAVPDYDEELEGALHW
                     TELHLSGDVEMSIEASAMVLLAGPCAEARDRRLRFDRVFAGEAATDDREAVASLGLSD
                     DQFVAASREALAIIERDWALIERIADELVKGGLLEFDEVEAIVLGAGG"
     gene            complement(4420..4899)
                     /locus_tag="VIV63_06390"
     CDS             complement(4420..4899)
                     /locus_tag="VIV63_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124260.1"
                     /translation="MRHSSLFPLLTLLATPTFAVDSTCEIYLTAAEKSAQQPARHTVT
                     EPGDGSSLEAIILDGQFFAKLEGKWMKLPGSPGLQGERKLVASIRSGKYPMTGCRKLD
                     SDRIDGMPTTVISYVLELPGNPAEETRAYIGADGLVHQQTSGKTRVRHRYTGVRAPM"
     gene            complement(4986..>5217)
                     /locus_tag="VIV63_06395"
     CDS             complement(4986..>5217)
                     /locus_tag="VIV63_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124261.1"
                     /translation="RKVDDALRESGTALAKWRRVSGFRDVRMEPYLQRVRADALAANG
                     QLAAAQSLEDLAATASARYDAAESPSTQRRQF"
BASE COUNT          963 a         1620 c         1693 g          941 t
ORIGIN      
        1 agcccggtga gcgggcgcgg gcctccgacg tcaacggcaa cttcaacaac ctcaagatcg
       61 cggtcgaagc cgcggagcgg cgcaacaccg ggttgcagaa ccgcattcgc gatctggaag
      121 cggcgctctc gaacgtgcgc acgctcgacg ccgtgttgac agtcgaaacc atcaatggag
      181 tgcgcacggt gcgcctgacc ggcgtcaacc tgcaggtggt caacggcctc tcgcgcaccg
      241 agacgatcaa tggtgcgggc aacgtcatca tcggctacga cgaagcgaac agtttcacga
      301 cgcgtccgat ctgctcccag gcgagcgcgc tcaatggaac ggcgatcacc gacgaagcag
      361 ggtgtctcgc ggcgggcggc gtgttctcga cacagcacaa atccggttct cacaacctgg
      421 tgctcggcac gcagaatggt tactcctcgt atggcggcat cgtcggtggc cgtttcaact
      481 tcatcaacga gatctttgcg accgtcgtgg gtggcgtcga caatcgcgcc agtggccggt
      541 tctcggcgat cgtcgcgggg caggggaatc tcacgcagga cagcgccgcg atactcggcg
      601 gaaacggcaa ccaggcgttg gcgcgcaata gttcgatcac cggcggcatc ggcaatgtgg
      661 cgagcggtga caacgcctcg atcaccggcg gcgagcgcaa caccgtgtcg ggccgtgctt
      721 cgacggtgtc gggtggtctt ctcaatacgg cttcgggttc gggagccaat gtcagcggcg
      781 ggcgcgacaa cgtggcctcg ggaacccata gcagcattct cggcggcagc ggtcgcacgc
      841 tggccggcgc caacgcgtcg cagccgtaag ggggcgccat gagaacattt gccaggcttg
      901 cgatcacgct gctcgcggca agtttgttag gggcttgcgg cggatccggc gagggtgccg
      961 cgccggccgg cgactcaccg gcggtcgccg agtcccatgc ctgggacagc tggaactggg
     1021 acgagggttc gttcggcgac tgagcggttc cggttgactt caggatcggt gaatgtgtat
     1081 atacacattc atgaatatat cgaccttcgc cgctctcgcc gatcccatgc gtctcgcgtt
     1141 gatcgaggca ttgcgtgacg gcgaacagcc ggtcaacgaa ctcgtcgagc gcgtccgcat
     1201 ccagcaatcc ggcgtctcgc gccatctcgg aatattgagc cgcgccggtc tggtcagcgt
     1261 gcgccccgag ggccagcaac gtttctattc attgcgccag cagccattcg acgaactcga
     1321 cgaatggatc gcgcgcattc gcgcccattg ggaagcacgg ttcgaccgct tcggcgccga
     1381 gctcgagcga cgcaaggcgg ctgatacaac caagaagaag cggagcacca agtgaaaaaa
     1441 cgcatcaacc tgcagcgcac gttcaacgcg ccgctcgatc tcgtctggga actgtggacc
     1501 acgaaagagg gcatcgagtc ctggtggggg ccgccgggat tcgaagtgaa ggtcagcaag
     1561 atcgagctgc gcgctggcgg cgcgctcgaa tacgtcatga cctgcgtggg cgccgaccag
     1621 atcgctttca tgaagcagca caaccagcca ctcaccacgc cgctcaaggc gcgctacacc
     1681 gaagtcagtc cgaagacgct ggcggcgtgg atgaatctcg cggatttcat tccgggcgtc
     1741 gatccgtacg aggtcgagac gcgcctcctg ttgacggcgc tgggcgcgga ctccgtgcgt
     1801 gtcgatctcc agctcgacgc catgcacaac gagttctaca catcgatggc cacgcagggc
     1861 tgggagagcg agatgggcaa gctcgccgct gtcatcgaca gccggaggaa gtcatgaagg
     1921 cattgatacc gttcctgtgg ttcgccgacc aggcggaaga ggccgcgcgc tactacgtcg
     1981 gcatcttccc gggcgcgaag atgggtgaga tccggcgctt tccggccaac agtcgcggcc
     2041 ccgagggcgc tgtcatgacc gccagcttca cgctgggcga tcagacgctg gtcgcgctca
     2101 atggcaatcc acggttcacg ttctcgccgg cgatgtcgtt gctgtatccc tgcgccagca
     2161 gcgacgaagc cgcggcgctc acggagaaac tcgccgaagg cggcgagaag cagccgggtg
     2221 gctggctgcg cgacaagtac ggcgtgtcgt ggcaggtcca cgcgattagc taggaagcgc
     2281 gcgctttcgg ggcgacgcgg atgttgacat cgagcaattc cggcgcgagg ttggagatgc
     2341 gctcgacaaa actccgcgtg acttcgaatc ctttcggcgc gagcagtatg cccatccgag
     2401 tgcgcaactc ctgcaggacg gtcattccgg gaacgactcg gcgcagcgga atctcgagcg
     2461 aatcctgcga gctgtcgtcg gtcgccggac aatgtccgag ctgaccgaag agttttgcgc
     2521 cgaagcgccc cacccggaag ctcagtgatt gcaatgcggc gtccttggat ttcccctgcg
     2581 cctggagcgc atcgaactcg agggtcaggc caaggatgcg ggtgccgaga ccaatggtgc
     2641 cttcgccgag gcgcgtgatc tgcgcgtcgg tccacgtgag cgccgtcagg atctgcagca
     2701 ccggatcgag acgcgggatg tgatcgagta gcttgatggc gaccgcgggt actccgttgg
     2761 cgagcgtctg ctcatcgggt gtcagcggca caccctgctg gaccttctcg acgacttcag
     2821 gtggcaacga tagatagccc acctgcgaca gcaatgctgc ggcctctagc tgccagaaat
     2881 cggtgcagcc aatccgcccg gcaagttcgc acgccagccg cttgatgtga ttggcgcgac
     2941 cgaaggcggc cggattcgcc agcgcgagca cctccatgag tgcgccgatg cagccgatca
     3001 gcgtttcctg gagcacggcc cgctcggcgt tgagcaggcg atgctgggcc actgcggcct
     3061 cgagcgccgt tttgaggtct tcgatggggc agggcttcga cagaaagcgg aatatctgcc
     3121 ccttgttgac ggcgagaatg gcggcatcac gccccgcctc gccactcagg agcatccgga
     3181 tagcgtgcgg acagcgttgc atggccgcct gcagaaacgt cgcgccatcc atctgcggca
     3241 tgcgcatgtc cgagatcacg accgccagtt gctgcagttc gcgcattttc tgcagcgcct
     3301 gttcaccgct ggtcgcgacg tgcacctcgt agtcctttcg cagcagcacg acaagccctt
     3361 cgaggactcg cggttcatcg tccacgaaca ggattttggg ccgcgatcgc accgccgggt
     3421 ctgattccat cccttcagta tccagagcca gcccgttccc cgagcgtgga atgtacgtaa
     3481 aaagagaatc aatggaacaa acggcgggcc ccaaacgtat tcagtgtagg gacgcgctta
     3541 cgaggaattt gatgcaaacc gcttatcgag atgaacagga gctccgccgc tgcgcccgca
     3601 cggcgctgaa cgagggcctg atccctcgcg aacgcccgaa tagcctgtgg ggcggacatg
     3661 ggaccggaga tacctgccca gtgtgcgcgc aagccatcga tgcgaccgag atggagctcg
     3721 aggtggagtt cgccgaggac tgcgcgcacg gcgtgcgcga tttccatctg cacctgccgt
     3781 gtttcgccgc ctgggaaatc gaaaggaagg tggcgcccga gtaagagctg cgcgactacg
     3841 ggtcagccgc ccgcgcccag aacaatggcc tcgacttcgt cgaactcgag aaggccgccc
     3901 ttcacgagct catcggcaat gcgctcgatg agcgcccagt cgcgctcgat gatcgcgagc
     3961 gcttcgcggc tggcggcgac gaactggtca tcgctcaggc ccagcgatgc cacggcctcg
     4021 cgatcgtcgg ttgccgcttc gcccgcgaac acgcgatcga atcgcaggcg ccggtcgcga
     4081 gcttcggcgc aggggcccgc gagcaacacc atcgcggatg cttcgatcga catctcgacg
     4141 tcgcccgaca ggtgcaactc ggtccagtgc aacgctcctt cgagctcttc gtcgtagtcc
     4201 ggcacggcgg tgacgtgatc gaaaacgagg ccggcacgaa tcgccgcgac ggcgtgcgca
     4261 gcttcatgga tcgccgtcgt tctggagatg tgcatgttcg tcgcggtccc acccgttact
     4321 gtcgtatccg atatggtgcg tagcgtggcg ttccttatgg acgttgtcga tgcccgcgta
     4381 gccgggagtc attcgcgcac ggtggtgtcc gccaggtcac tacattggcg cccgaacacc
     4441 ggtgtagcgg tgtcgcaccc gggtctttcc ggacgtctgc tgatggacga gtccatcggc
     4501 gcctatgtag gcgcgggttt cctcggcagg attgccgggt agttcgagta cgtatgagat
     4561 caccgtggtc ggcattccgt cgattcgatc agagtcgagt ttgcggcagc ccgtcatggg
     4621 atatttcccg gaacgaatcg acgctacgag cttgcgttca ccctgcaacc ccggactgcc
     4681 cggcagtttc atccacttgc cttcgagctt cgcgaagaac tggccgtcga ggatgatcgc
     4741 ctcgaggctg ctcccatcac ctggctcggt gacagtgtgt cgggcaggtt gttgcgcgga
     4801 tttctcggcg gccgtcagat agatctcgca cgtggaatcg acggcgaaag tcggggtggc
     4861 gagcaatgtg agcaacggaa agagggatga gtggcgcatg gtggtttcct cggggtgaat
     4921 gcactctcac ccgaacgcct tctgcgcgcg gaaccggaca tgacgtccgc agtggggctc
     4981 ggaattcaga attgccttcg ttgcgtgctc ggcgattccg cagcgtcgta acgcgcgctc
     5041 gccgtggcag ccagatcttc gagtgactgg gctgctgcca gctgtccgtt cgcggcgagt
     5101 gcatctgcgc gcacacgctg aaggtacggc tccatgcgca cgtcgcgaaa tccggaaact
     5161 ctcctccact tcgcgagcgc cgttccactc tcacgcagcg cgtcgtccac ctttcgc
//
LOCUS       DATDEU010000263         8126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_630420, whole genome shotgun sequence.
ACCESSION   DATDEU010000263 DATDEU010000000
VERSION     DATDEU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630420"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VIV63_06400"
     CDS             complement(<1..1329)
                     /locus_tag="VIV63_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY6124262.1"
                     /translation="MNKSTRRDALKSLLVGGAVVATPAADAKSRSKAPDACAAALRTD
                     WSDLRWGKGFEGQRIADLGDGTFLNPVFSGDRPDPTILKDGDDYWMTFSSFDSYPGIV
                     VWHSRDLVNWNPVGPALTKPVGSVWACDIARHGGRYFIYFPARTAGRRSNYVVHAASM
                     RGPWSDPIDLNLPAHIDPGHIVGEDGKRYLFLSGGDRVKLKDDGLSTDGAVEHVYDPW
                     RYPDDWVVESFSPEGPKVLKHGQYFYMVTAVGGTAGPPTGHMVIAARSKSINGPWENC
                     PANPLVRTTDAREKWWSRGHASLVEGPAGDWWSVYHGYENAYWTLGRQTLLDRIEWTD
                     DGWFRFLGKDLSAPIPKPAKLRNQSHGIALSDDFAKDKFGIQWAFYAPGDNERARVAY
                     DNGALVMTGKGDSPSNCSPITCITGDQAYRCEVEIEVEGAATGGVLFFYNK"
     gene            complement(1376..1642)
                     /locus_tag="VIV63_06405"
     CDS             complement(1376..1642)
                     /locus_tag="VIV63_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124263.1"
                     /translation="MDRKIAEQIVALVNGSIDQLIATLEPVEAAVSPEEFAAYKRGIA
                     KVIAAFDTGVIDLVAREYPDLKPADDDSDHATPPESDLPKASRN"
     gene            complement(1684..3519)
                     /gene="glmS"
                     /locus_tag="VIV63_06410"
     CDS             complement(1684..3519)
                     /gene="glmS"
                     /locus_tag="VIV63_06410"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY6124264.1"
                     /translation="MCGIVGAIAERNVVPILMEGLRRLEYRGYDSAGIAVLNGTAKLK
                     RLRTVGKVKMLEQAIEADPTHGQVGIAHTRWATHGVPSERNAHPHISHDGLAIVHNGI
                     IENHEELREELKKAGYTFTSETDTEVIAHRVHFHLAAQKDLFKAVRATVSELEGAYAL
                     AVVSEAEPERIILAREGCPVVVGLGVGENFVASDVAALLPVTRRFMFLEEGDVADIHR
                     TSIKVLDKNGDTAERPVRESELSADAAEKGQYRHFMLKEIHEQPRAVADTLAERVANG
                     RLLEAAFGPAATQIFKHTEHVHIIACGTSYHSGVVARYFIEQICKIPCTVEIASEYRY
                     RNPVVPKNSLFVTISQSGETADTLAALRLAKQAGYLSSLAICNVPESSLVRESELVML
                     TRAGPEIGVASTKAFTTQLTALSMLVIALAKQRTADAERERSLVQRLIEIPSVIEKTL
                     ALDPIIYDLAKRFADKHHALFLGRGALHPIAMEGALKLKEISYIHAEAYPAGELKHGP
                     LALVDADMPVITVAPNNDLLEKLKSNLMEVRARGGELIVFADPDSGFVSSDGVTVIQM
                     PKHVSYFQAPAVYTVPLQLLAYHVAILKGTDVDQPRNLAKSVTVE"
     gene            complement(3676..4101)
                     /locus_tag="VIV63_06415"
     CDS             complement(3676..4101)
                     /locus_tag="VIV63_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124265.1"
                     /translation="MMVGALLCIAGAIYGLLGALHALYTFLDMRDPRRIVPDDPAVMT
                     AMQNSKIRLTRGESTMWQGWVGFNLSHSLGALMLSAACFIVVACFRIVAFSPWALFAL
                     AAVSSLYLLMAAQYWFRIPILGTATATACLAAAWLLYLL"
     gene            complement(4098..4547)
                     /locus_tag="VIV63_06420"
     CDS             complement(4098..4547)
                     /locus_tag="VIV63_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124266.1"
                     /translation="MFPEGAQTPPAHTHPLWFAALVAAVSAPLAYWIVLAAIELWIGG
                     GSISDVIMRTLGMVAMIGVPVSIIAMFVFGYPLALLLRKLGTLSALSLCTGAVVIGAA
                     FAAGIAKMFFPTNPVDVVTPLLGAATGLFAGIVFCLVAGIRFRRSPR"
     gene            complement(4537..5139)
                     /locus_tag="VIV63_06425"
     CDS             complement(4537..5139)
                     /locus_tag="VIV63_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6124267.1"
                     /translation="MTLRVLGRKTSINVRKVLWTCAEADLKFDHVESDAKLLAQNPNA
                     MVPVIQDGDFVLWESNAICRYLAGKQARSTLLPEDPQARAVVEQWMDWQASELNVAWR
                     YAFLGLARKSPAHADPQAIAASVESWNRHMRILDEHFTQGGQFVTGEFFTLADVVLGL
                     STQRWLLTPMDRPHLDAVHGYYQRLSVRPAFRAQIDLNVP"
     gene            complement(5146..5463)
                     /locus_tag="VIV63_06430"
     CDS             complement(5146..5463)
                     /locus_tag="VIV63_06430"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEY6124268.1"
                     /translation="MKRSLAMASVLFLLPLAASADESFRCGNWIASSSMSVEELLQKC
                     GEPTSRSTQTEDVMVRNRDNGLMTKVGETQIETWIYERGSQAAAMVVTIVDGRIKKLE
                     RQK"
     gene            complement(5531..5917)
                     /locus_tag="VIV63_06435"
     CDS             complement(5531..5917)
                     /locus_tag="VIV63_06435"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEY6124269.1"
                     /translation="MSVRIVRLGTPRAANEGTRLGTVRRPPRGVPRKDFGRLDWYDVW
                     FPVLAPSAAIMKLGLSADSEREWKAFARQYRAEMAAPAASQSLDVLAALSHHADFSVG
                     CYCENPERCHRSLLRELLVERGAKLE"
     gene            complement(5922..6389)
                     /locus_tag="VIV63_06440"
     CDS             complement(5922..6389)
                     /locus_tag="VIV63_06440"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY6124270.1"
                     /translation="MRIDITERQPVKVAYLRYTGPFGEPLGRFWRGPVATWLAELGML
                     DCPRYGVTLDNSLNTPPEKCRYDACVEVPKGMTLPGAGETVIAGGRYAVTHFKGNGAE
                     IGAAWGAFVIAALADPANRADPARHPFEHYPRGAVFDGKTGVFACELCLPLAE"
     gene            complement(6399..6914)
                     /locus_tag="VIV63_06445"
     CDS             complement(6399..6914)
                     /locus_tag="VIV63_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY6124271.1"
                     /translation="MPETALICRVPESERYIAHYRQRFDPSARRNVPAHVTILYPFLA
                     PDLIDTEVLATLTGIANSVPCFSYRLAKIERFPVAMYLAPDPPVHFAALTDGVFRAFP
                     DYPPFEGKFDTVVPHVTVAHGDEPLLCELEVELRIALPGAGVQARCSELVLIENSSGR
                     WEQMHVFALGA"
     gene            complement(6907..7158)
                     /locus_tag="VIV63_06450"
     CDS             complement(6907..7158)
                     /locus_tag="VIV63_06450"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEY6124272.1"
                     /translation="MLQLRPNCECCNTDLAGDATDAYICSFECTFCARCTHEKLQGRC
                     PNCGGELLRRPPRAADKLMKYPASAERVYKPEGCGKPVA"
     gene            complement(7173..7676)
                     /locus_tag="VIV63_06455"
     CDS             complement(7173..7676)
                     /locus_tag="VIV63_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124273.1"
                     /translation="MKRILLSMCAAGMAVSAGAASTWCLGLNGLGPIRAGMTVEQVMR
                     LADFSGLERKHPAGECWYLNYRGGSSAFDLMIIGGAVVRLELKRDSKLHTFAGARIGT
                     TEEELKALYGARLDIQPHKYDTNGHTITMKSSGGEYGLRFETAGGRVTAIQSGPWEHL
                     HYVEGCG"
     gene            complement(7673..>8126)
                     /locus_tag="VIV63_06460"
     CDS             complement(7673..>8126)
                     /locus_tag="VIV63_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY6124274.1"
                     /translation="EKHYEAFPAEFVHWAPASWDGVPSEAFTAIEQICHVRDIEVEGY
                     QVRFRRTLDELSPLLPSIDSETVAKQRNYGSADPATVFADFRAAREQTLVLLRRLSEP
                     DLDRPAVFEGYGPVTMRALIHYLCSHDQQHLAGLQWLLGKIDAERHRT"
BASE COUNT         1433 a         2502 c         2740 g         1451 t
ORIGIN      
        1 cttgttatag aagaagagaa cgccgcccgt ggccgcgccc tcgacttcga tctccacctc
       61 gcagcgatag gcctgatcgc cggtgatgca ggtgatgggt gagcagttag aggggctatc
      121 gcccttgccg gtcatcacca gcgcgccgtt gtcgtacgcg acgcgtgcgc gctcgttgtc
      181 tcccggcgcg tagaacgccc actggatgcc gaatttgtct ttggcgaaat cgtccgagag
      241 tgcgatgccg tgcgattgat tcctgagctt cgcgggtttc gggatcggcg cggagagatc
      301 cttgccgaga aaccggaacc agccgtcgtc cgtccactcg atgcggtcga gcagcgtctg
      361 gcggccgagc gtccagtacg cgttttcgta gccgtgataa acgctccacc agtcgcctgc
      421 aggaccttcg accagcgagg cgtggccgcg tgaccaccat ttctcgcgcg catcggtggt
      481 gcgcacgagc gggttggccg ggcagttttc ccacgggccg ttgatcgact tcgagcgcgc
      541 ggcgatgacc atgtggccgg tgggcgggcc cgcggtgccg ccgacggcgg tcaccatgta
      601 gaaatactgg ccgtgtttga ggaccttcgg cccttcgggc gaaaagctct cgacgaccca
      661 gtcgtcggga tatcgccagg ggtcgtagac gtgctccacg gcgccgtcgg tagacaggcc
      721 gtcgtccttg agcttcacgc ggtcgccgcc ggagaggaac aggtagcgct tgccgtcctc
      781 gccgacgatg tggccgggat cgatgtgcgc cggcaggttg aggtcgatcg gatcgctcca
      841 cgggccgcgc atcgacgccg catgcacgac gtagttagac cgcctgccgg ccgtgcgcgc
      901 cgggaagtag atgaagtagc ggccgccgtg tctggcgatg tcgcaggccc agacggagcc
      961 gacgggtttg gtcaaagccg gaccgacggg attccagttc acgaggtcac gcgagtgcca
     1021 gacgacgatg cccgggtacg agtcgaatga cgagaacgtc atccagtagt cgtcgccgtc
     1081 cttgaggatg gtgggatcgg gccggtcgcc cgagaacacg gggttgagga aggtcccgtc
     1141 gccgagatcg gcgatacgct ggccttcgaa gcctttgccc cagcgcaggt ccgaccagtc
     1201 ggtgcgcagg gctgcggcgc aggcgtcggg ggctttcgat ctggatttcg cgtcggcggc
     1261 gggagtggcc acgacggcgc cgccaaccag caatgatttc agcgcatcgc gccgcgtgct
     1321 cttgttcatc gcggcacggt aaccggtgtc aggctggcct ggcaacggcc gggaatcagt
     1381 tgcgcgaagc cttgggcaga tcagattcgg gtggcgtggc gtgatcggaa tcgtcgtcag
     1441 ccggcttcag atccggatat tcgcgtgcca ctagatcgat gacgccggtg tcgaaggcag
     1501 cgatgacctt ggcgatgccg cgcttgtagg ccgcgaactc ttcgggcgag acggcggctt
     1561 caacgggctc gagagtggcg atcagctggt cgatgctgcc gttcacgagc gcaactatct
     1621 gctccgctat cttgcgatcc atgaggtccg gccccttttg ctaggaaatg gggagtgtcc
     1681 ccattactcg acggtgacgg acttcgcgag gttgcgcggc tgatccacgt ccgtcccctt
     1741 gaggatggcg acgtgatacg caagcagctg cagcggcacg gtgtacacgg ccggcgcctg
     1801 gaagtagctc acgtgcttcg gcatctggat gacggtgacg ccatcggacg aaacgaagcc
     1861 cgagtccggg tcggcgaaca cgatgagctc gccgccgcgc gcgcgcactt ccatgagatt
     1921 gctcttgagc ttctcgagga ggtcgttgtt gggcgccacg gtgatgacgg gcatgtccgc
     1981 atccacgagc gcgagcgggc cgtgcttcag ctcgccggcg ggataggcct cggcgtggat
     2041 gtaggaaatt tccttgagct tgagcgcgcc ttccatcgcg atgggatgca gagcgccgcg
     2101 cccgaggaac aacgcgtggt gcttgtcggc aaaacgcttg gcgaggtcgt agatgatcgg
     2161 atcgagcgcc agcgtcttct cgatgacaga aggaatctcg atgagccgct gcacgaggct
     2221 gcgctcgcgc tcggcatcgg ccgtgcgctg cttggcgagc gcgatgacga gcatgctgag
     2281 cgcggtgagc tgcgtggtga acgccttggt cgacgcaacg ccgatttccg gaccggcgcg
     2341 cgtgagcatc accagttcgg attcgcggac gagcgagctc tccggcacgt tgcagatggc
     2401 cagcgacgac aggtaacccg cctgcttcgc gagccgcagc gcggccagcg tgtcggcggt
     2461 ttcgccggac tgggagatgg tcacgaacag cgagttcttg ggcaccaccg ggttccggta
     2521 gcggtattcg ctggcgattt cgaccgtgca ggggatcttg cagatctgct cgatgaaata
     2581 acgcgcgacg acgccggagt ggtagctcgt gccgcaggcg atgatgtgaa cgtgttcggt
     2641 gtgcttgaag atctgcgtcg cggcgggacc gaaggcggcc tccagcaagc ggccattggc
     2701 cacccgctcg gccagcgtgt cggcgacggc gcgcggttgc tcgtggattt ccttgagcat
     2761 gaagtggcgg tactggccct tctcggccgc atccgcagac aactcgctct cgcgcacggg
     2821 acgctcggcg gtgtcgccgt tcttgtcgag caccttgatc gaagtccggt gaatgtcggc
     2881 gacatcaccc tcttccagga acatgaagcg gcgcgtcacg ggcagcagcg cggcgacgtc
     2941 ggaggccacg aagttttcgc cgacgccgag gccgacgacc accgggcagc cttcgcgcgc
     3001 caggatgatg cgctcgggtt ctgcctcgct aactaccgcc agcgcgtacg ccccttcgag
     3061 ttccgagacg gtggcgcgga cggccttgaa gagatctttc tgcgcggcga gatggaagtg
     3121 cacgcggtgc gcgatgactt cggtgtcggt ttccgacgtg aacgtgtaac cggccttctt
     3181 cagttcttcg cggagctctt cgtgattctc gatgatgccg ttgtggacga tggcgaggcc
     3241 gtcgtgcgag atgtgcgggt gcgcgttgcg ctcggaaggc acgccgtgcg tggcccagcg
     3301 cgtgtgcgcg atgccgacct ggccgtgcgt gggatcggct tcgatggcct gctcgagcat
     3361 cttgaccttg ccaacggtgc gcagacgctt gagcttcgcc gtgccgttga gcaccgcgat
     3421 gccggcggag tcgtagccgc ggtattcgag gcggcgcagt ccctccatga ggatgggaac
     3481 gacgttgcgc tcagcgatgg ctccgacgat gccgcacatt taacagtgtc cgaaaatttg
     3541 ggggcggcta gtgtgcctga gtgcagccac ggaacgcagt ggcaaaccac cccttaaagg
     3601 gacttctgat accccgctca caaacggtcg ctaaatgagc caactggtat tacgaatcaa
     3661 caccttacgg gtgccctaga gaaggtaaag cagccaggcg gcggccaggc aggccgtggc
     3721 ggtcgccgtt cccaggatcg ggatgcgaaa ccagtattga gcggccatca gcaggtacag
     3781 cgagctgacg gcggccagcg caaacagcgc ccagggcgag aacgcgacga tgcggaaaca
     3841 ggccacgacg atgaagcagg cggcggagag catcagtgcc ccgaggctgt ggctcaggtt
     3901 gaagcccacc cagccctgcc acatggtgga ttcgccgcgc gtcagccgga tcttcgagtt
     3961 ctgcatggcg gtcatgaccg ccggatcgtc gggcacgatg cgccgcggat cgcgcatgtc
     4021 gagaaacgtg tacagcgcgt gcagtgcccc taacaagccg tagatggcac cggcgataca
     4081 cagaagcgcg ccgaccatca tcggggtgac cgcctgaagc gaatgccggc gaccaggcag
     4141 aacacgatgc ctgcgaacag gcccgtggcc gcacccagaa gcggggtcac aacatcgacg
     4201 ggattggtgg ggaagaacat ctttgcgatc ccggccgcga atgcggcgcc gatgacgacg
     4261 gcgccggtgc acagactgag cgccgacagc gtgcccagct tccgaagcaa cagcgccagt
     4321 ggatagccaa acacgaacat ggcgatgatg gacaccggaa cgccgatcat cgccaccatg
     4381 ccgagggtgc gcatgatcac gtcactgatc gatccgccgc cgatccaaag ctcgatggcg
     4441 gcgagcacta tccagtaggc gagtggcgct gacaccgcgg cgacgagcgc ggcgaaccac
     4501 aaaggatgtg tatgcgccgg cggcgtctgc gcgccctcag ggaacattca gatcgatctg
     4561 cgcccggaac gcggggcgca ccgacaggcg ctggtagtag ccgtgaaccg cgtcgaggtg
     4621 cggccgatcc atgggcgtga gcagccagcg ctgggtagat agccccagca ccacgtcggc
     4681 cagcgtgaag aattcgccgg tgacgaactg cccgccctgg gtgaagtgtt cgtcgaggat
     4741 gcgcatgtgg cggttccagc tctcgacgct ggcggcgatc gcctgcgggt ccgcgtgtgc
     4801 cgggctcttg cgcgccaggc ccagaaacgc atatcgccag gccacgttga gttcggaggc
     4861 ctgccagtcc atccactgct cgacgaccgc gcgagcctgc ggatcttccg gcagcagcgt
     4921 ggatcgggct tgtttgcccg cgagatagcg gcagatggca ttcgattccc acaacacgaa
     4981 atcgccgtcc tggatgaccg ggaccatcgc attgggattt tgcgcgagca gcttcgcgtc
     5041 ggattcgacg tgatcgaact tgaggtcggc ttcggcgcag gtccacagca ccttgcgcac
     5101 gttgatggac gtttttcggc ccaggacccg gagtgtcatt gcgcgttact tctggcgctc
     5161 gagcttcttg atgcggccat cgacgatggt gacgaccatc gctgccgcct gcgacccgcg
     5221 ctcgtagatc caggtttcga tctgcgtttc gccgaccttc gtcatcaatc cgttgtcacg
     5281 gttacgcacc atgacgtctt cggtctgggt gctgcgcgag gtcggctcgc cgcacttctg
     5341 aaggagctcc tcgacggaca tggaactgga ggctatccag tttccacagc ggaagctttc
     5401 atcggcggag gccgcgaggg gcagtagaaa cagcaccgaa gccatggcga gtgaacgttt
     5461 catggccggg atggtagcac cccgttcatt cggggacgac gcctgtcaag actgtactca
     5521 cgtttgctaa ctactccagt ttcgcgcctc tttcaaccag gagttcgcgg agcagtgaac
     5581 ggtggcaacg ttccgggttc tcgcaataac aacccaccga gaaatcagcg tgatgagaca
     5641 gcgcagccag cacatcgagc gactggctgg cggcgggggc ggccatttcc gcccggtatt
     5701 gtcgggcgaa ggctttccat tcccgctcag agtccgcgga cagcccgagt ttcatgatgg
     5761 cggcgctggg cgccagcacc gggaaccaga cgtcgtacca gtcgagccgt ccgaagtcct
     5821 tcctgggcac ccctcgcggt ggtcgtcgca cggtgccgag ccgcgtgccc tcgtttgcag
     5881 cgcgcggagt gcccagccgc acgatgcgca cgctcacgcc gtcactccgc caagggcagg
     5941 cacaactcgc aggcgaacac gccggtcttg ccatcgaaca cggcgccgcg cgggtagtgt
     6001 tcgaacggat gccgtgccgg atccgcacga ttcgccggat cggcgagcgc ggcgatcacg
     6061 aacgcgcccc aggcagctcc gatctccgcc ccgtttccct tgaaatgcgt gacggcgtat
     6121 cggcctccgg caatgactgt ttcgccggcg ccgggcagcg tcatgccttt cggcacttcg
     6181 acacacgcgt catagcggca tttctcgggt ggcgtgttca gggagttgtc gagcgtcacg
     6241 ccgtaacgcg ggcaatcgag catgccgagt tccgcgagcc aggtggcgac gggcccgcgc
     6301 cagaaacgcc cgagtggttc gccaaacgga ccggtgtagc gaaggtaggc aactttgacc
     6361 ggttgccgct cggtgatgtc tattcgcacg cgacgagttt acgcgccgag cgcaaacacg
     6421 tgcatctgct cccagcgtcc gctcgagttc tcgatgagca ccagctcgga acagcgcgcc
     6481 tgcacacccg cgccgggcag cgcgatgcgc aactccactt cgagttcgca caacagcggc
     6541 tcgtcgccgt gcgcgacggt aacgtgcggc accacggtgt cgaacttgcc ctcgaacggt
     6601 gggtagtcgg ggaatgcacg aaacacgccg tcggtgagcg cggcgaaatg cacgggggga
     6661 tccggggcga ggtacatggc cacagggaat cgctcgatct tcgcgaggcg atagctgaaa
     6721 cacggcacgg agttcgcgat tcccgtgagg gtggccagca cctcggtgtc gatcagatcc
     6781 ggcgcgagga atggatagag gatggtgacg tgcgccggca cattgcgccg cgcggaggga
     6841 tcgaatcgct gacggtaatg cgcgatatag cgctcggact ccggcacacg gcagatgagc
     6901 gcggtctcag gcaacgggct taccgcaacc ttcgggcttg tacacgcgct cggccgacgc
     6961 cgggtacttc atgagtttgt ccgccgcgcg cggcgggcgc cgcagcagtt cgccgccgca
     7021 gttggggcac ctgccctgaa gtttttcgtg tgtgcatcgc gcacagaacg tgcactcgaa
     7081 cgagcagatg tacgcatcgg ttgcgtcacc cgcgagatcg gtgttgcagc attcgcaatt
     7141 gggccggagt tgcagcatgg gatctcccgg cgtcagccgc atccttcgac gtagtggaga
     7201 tgttcccagg ggcccgactg gattgccgtc acgcggccgc ccgcggtttc gaagcgcaag
     7261 ccgtattcgc cacccgatga cttcatcgtg atggtgtggc cgttcgtatc gtacttgtgc
     7321 ggctggatgt cgagccgtgc gccatacagg gctttcagtt cttcttcggt ggttccgata
     7381 cgcgcgccgg caaaggtatg cagcttgctg tcgcgcttga gctccaggcg tacgaccgcg
     7441 ccgccgatga tcatgagatc gaaggccgaa ctaccgccgc ggtagttcag gtaccagcac
     7501 tcccccgcgg gatgtttgcg ctcgagcccg gagaagtcgg cgaggcgcat gacctgctcg
     7561 acggtcatgc cggcgcggat gggtccgagg ccgttgagcc ccaggcacca cgtagaggct
     7621 gcacccgctg agaccgccat tcccgccgcg cacatcgaca gcaggatccg tttcatgtcc
     7681 gatgcctttc cgcgtcgatc ttgcctaaca accattgcag gcccgccagg tgctgctggt
     7741 cgtggctgca gaggtagtgg atcagggctc gcatcgtgac gggtccgtaa ccttcgaaca
     7801 ccgcggggcg gtccaggtcc ggttcggaaa gccggcgaag cagcaccagc gtctgctcgc
     7861 gcgccgcgcg aaaatccgcg aacaccgtgg cggggtccgc gctgccgtag ttgcgctgct
     7921 tcgccacggt ctcgctatcg atggacggca gcagcggcga cagctcgtcg agcgtgcggc
     7981 ggaaccgcac ctggtaaccc tcgacctcga tgtcacgcac gtggcagatc tgctcgatgg
     8041 cggtgaatgc ttcgctgggc acgccatccc acgatgccgg cgcccaatga acgaattccg
     8101 ccggaaaagc ctcgtaatgt ttctcg
//
LOCUS       DATDEU010000264         4362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1797292, whole genome shotgun sequence.
ACCESSION   DATDEU010000264 DATDEU010000000
VERSION     DATDEU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797292"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3419
                     /locus_tag="VIV63_06465"
     CDS             <1..3419
                     /locus_tag="VIV63_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124275.1"
                     /translation="YTEQLFDGYDNGFHGDGEPLALASMTHARGVALDAQGNVYIADT
                     DAHRVRKITAINGVINPEESIITTIAGNGQWYSEEENVPATSTALSFPEALAIDAAGN
                     VYVHERGSNRVRRISVADGTISTYAGGAPWGYTGDGGPATEAHIELINGNIVLDAASN
                     LYLAEMGANRLRRVNAATGIIDTVLGNGTMTFCKEVSPRREACLGGPQGVAVDAGGNV
                     YISDSRNLRIRRIDASTGELRTIAGNSFGYTHGGDGGPATAASFAAEPGGISFDSAGN
                     LLIAGSWANNVRRIDMTTGIITTIAGTGVQGFAGDGGPATAARFASVQDVVADAAGNL
                     YLSDSYNYRIRKISAAGIVSTIAGNGINAFGGDGGPATAASLQWATDIRVDAAGNVFF
                     ADNARIRRIDAATGIIRTVAGNGGNWSAGEGVPATYVGIGWSFAFDLDGSGNIIINTG
                     NYLRRVDAVTGLITSVGSSNGMNTPDGIGIQSPAAMKFGLGGLFVTDDNSDLVFRIDG
                     VPSGGPDTTPPVITYVVNGIAGVEDWYRSDVTVTFTVSDPESPILSMSGCHTRSLTHD
                     TTGGAFICRAGSIGGESSRSVTIRRDTVAPVVTPEVPSPPDAYGWYNRYVTLWFYTSD
                     ALSGVYSSDPDGALYMNQEGTGLTGQAVVTDRAGNTTTYTSPPINIDLYPPAVASSRP
                     GETGNNGWYTTDVPVDWSIWEPISPIRSSEGCDDFVLSTDTPGTTFSCTATSAGGTLT
                     GTLTLKRDATPPTLVFGSPSPAPNASGWHNGDVSFPFTTSDGTSGVASTSSSGPVVIT
                     GEGAGLTATVTVTDAAGNSATFTTPPVNINRAPPPTVTPVVTGTPGNNGWYRSNVQLS
                     WTLTGDILSSTGCGNSSVTSDTAGETFTCSATSAGGTASSSVTIKRDVTAPTLTFGTR
                     TPAANSRGWNNTNVSFPFTTADALSGVASTSSPSPVVITGSGVGLTGQVTVSDAAGNS
                     AVFTSAAVNIDRTVAVITPNIVGTLGTNGWYRSNVQVSWTIDELPGSIDWTSGCGTTN
                     RTTDTTGVSYSCGVQSAAGYTSRTVSIKRDATAPVVTITRPANNATYTVGQSVTSSFG
                     CTDARMQSCVGTTTNGAPLDTSSAGTKSFTVNALDLAGNTTTLVRTYTVQ"
     gene            complement(3454..>4362)
                     /locus_tag="VIV63_06470"
     CDS             complement(3454..>4362)
                     /locus_tag="VIV63_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6124276.1"
                     /translation="LALAKGYDGKDRSYLEAWGIGATGKEDALYTALAASQPDSDATK
                     WPATYADLVWRLTPRAAVPAFAARAGADVLSVSERSKATTALGFIPSSEAATALLDVA
                     QHGGSDIKQNSALWWLMNYKDSRWAGQGIDAELKRRGLYDPDTVAINEVTVPAPDEKS
                     TLPAVADIAKLRGDAKRGATAAGACLMCHRIQGKGVDYAPALDGFASRQTRDVVITAI
                     VTPSSDIAHGYEGTEITLQDGRKIHGLVLSGGNPLIVQSTGGVTQMIPASQVRERKRL
                     GRSLMLSAGQLGLSAQQVADIVAYLR"
BASE COUNT          778 a         1473 c         1389 g          722 t
ORIGIN      
        1 ggtacaccga gcagctgttc gatggctacg acaatggctt tcacggcgac ggtgagccgc
       61 tcgcgctggc cagcatgacc cacgctcgcg gagtggcgct ggacgcgcag ggaaatgtgt
      121 acatcgcgga caccgacgcc catcgtgtgc gcaagatcac ggcgatcaac ggagtgatca
      181 atcccgaaga gtcgatcatc accacgatcg cgggcaacgg ccagtggtac tccgaagaag
      241 aaaatgtgcc ggctacgtcc accgccctgt cgtttcccga agcgctcgcc atcgacgccg
      301 ccggaaacgt ctacgtccac gagcggggct cgaaccgcgt gcggcgcatc tcggtcgccg
      361 acggcaccat ctccacctac gccggcggcg ctccgtgggg ctacaccggc gacggcggtc
      421 ccgccacgga agcgcacatc gagttgatca acggcaacat cgtcctcgat gcggcatcca
      481 atctgtatct cgcggagatg ggcgcgaacc gtctgcgccg cgtgaacgcg gccacgggca
      541 tcatcgatac cgtgctcggc aacggcacca tgactttctg caaggaagtc tcgccacgcc
      601 gcgaagcctg cctgggcggg ccgcagggcg tcgccgtcga tgccggcggt aacgtctaca
      661 tttccgattc gcgcaacctg cgcattcgcc gcatcgacgc cagcaccggt gaacttcgca
      721 ccatcgcggg caacagcttc ggttacacgc atggcggcga tggcggaccg gcgacggccg
      781 cgagcttcgc ggcggaaccc ggcggcattt ctttcgactc ggccggtaac ctgctcatcg
      841 ccggaagctg ggccaacaac gtgcgccgca tcgacatgac gaccggcatc atcaccacca
      901 tcgcgggcac gggcgtccag ggcttcgcgg gtgacggggg tcccgcgacc gccgcgcgct
      961 tcgccagcgt gcaggacgtg gtggccgatg ccgccggcaa cctctacctc tccgattcct
     1021 ataactaccg gatccggaag atcagtgccg ccggcatcgt ttcgaccatt gccggcaacg
     1081 gcatcaatgc cttcggtggc gacggtggcc cggcaaccgc ggcgtcactg cagtgggcga
     1141 cggacatcag ggtcgatgcc gcgggcaatg tgttcttcgc cgacaatgcg cgcatccggc
     1201 gcatcgatgc ggccaccggc atcatccgga ccgtcgccgg aaacggcggc aactggagcg
     1261 cgggcgaagg cgtgcctgcc acctacgttg gcatcggatg gtcgtttgcg ttcgacctcg
     1321 atggctccgg caacatcatc atcaacacgg gcaactatct tcgtcgtgtc gacgccgtaa
     1381 ccgggctcat cacctcggtc ggctcgtcga atggcatgaa tacgccggat gggattggca
     1441 tccagtcacc cgccgcaatg aagttcggtc tgggcggcct gttcgtcacg gacgacaact
     1501 ccgatctcgt atttcgaatc gacggcgtgc cgagtggtgg accggacacc acaccgccgg
     1561 tgatcacata cgtggtcaac ggaatcgccg gggtcgaaga ctggtaccgc agcgacgtca
     1621 cggtgacgtt tacagtgagc gatccggagt caccgatact ttcgatgagc ggttgccaca
     1681 cgcgatcgct cacccacgac accacgggcg gcgcattcat ctgtagggcc gggtcgatcg
     1741 gtggcgagtc gtcgcgttcc gtcacgatcc gccgcgatac cgtggcgccg gtcgtgacgc
     1801 cagaggtccc ttcgcctccg gatgcctacg gttggtacaa ccggtacgtc acgctctggt
     1861 tctatacatc cgacgcactt tcgggcgtgt attcgtcgga tcccgatggc gcgttgtaca
     1921 tgaaccagga aggcaccggc cttaccggcc aagccgttgt gaccgaccgt gccggcaaca
     1981 ccacgacata cacctcgcca cccatcaaca tcgaccttta tccgccggcg gtcgcttcat
     2041 cgcggccggg tgagaccggc aacaacggct ggtacacgac tgatgtgccg gtggactgga
     2101 gcatctggga gccgatctcg cccattcgtt ccagcgaagg ttgcgatgac ttcgtgctga
     2161 gcaccgacac tcccggcacg acgttctcat gcaccgccac ctcggcaggc ggaacgctga
     2221 ccggcacgtt gaccctcaag cgtgacgcga cgccacccac gctggtgttc ggctcacctt
     2281 cgccggcgcc gaatgcatcg ggctggcaca acggcgacgt gagtttcccg ttcaccacgt
     2341 cggatggcac atccggagtc gcgagcacca gcagcagcgg acccgtagtg atcaccggag
     2401 aaggcgcggg actcaccgcc accgtgacgg tgaccgatgc agccggcaac tcggcgacct
     2461 tcaccacgcc gccggtgaac atcaaccgcg cgccgccgcc caccgtgacg ccagtcgtca
     2521 ccggcacgcc cgggaacaac ggctggtatc gcagcaacgt gcagctgagc tggacgctca
     2581 ccggcgacat cctgtcgagc acgggttgcg ggaacagcag cgtgacctct gatacagctg
     2641 gcgagacctt cacttgcagc gcgacttccg cgggcggcac ggcgtcgagt tccgtgacta
     2701 tcaagcggga tgtgacggcg cccactctca cgttcggcac gcggactccc gcggccaaca
     2761 gccgcggctg gaacaacacc aacgtgagct tcccgttcac gaccgcggac gcactgtccg
     2821 gcgtggcgtc gactagctcg ccgagcccgg tggtcatcac cggcagcggt gtgggactta
     2881 cgggccaggt cacggtgagc gatgcggccg gcaacagcgc ggtgttcacc tcggctgcgg
     2941 tcaacatcga tcgcaccgtg gcggtgatca cgcctaacat cgtgggcacg ctcggcacca
     3001 acggctggta tcgcagcaac gttcaggtca gctggacgat agatgaactc ccgggaagca
     3061 tcgactggac cagcggttgc ggcacgacga accggacgac ggacaccacg ggcgtgtcgt
     3121 attcctgcgg tgtgcagtct gcggccggtt acacgtcgag aacggtctcg atcaagcgcg
     3181 atgccaccgc gccggtcgtg acgatcacga ggccggcgaa caacgccacc tacaccgtcg
     3241 gccagagtgt gacgagcagc ttcggctgca ccgatgcgcg catgcagagc tgcgtgggaa
     3301 ccacgacgaa cggcgcgccg ctcgatacga gcagtgcggg caccaagtcg ttcaccgtga
     3361 acgcgctgga tctcgcgggc aacacgacca cgctggtccg cacgtacacg gtccagtagc
     3421 ggccacgtca acaaacgggg tcaggcaccg ttttcaacgt agataggcga cgatatccgc
     3481 gacctgctga gcgctgagcc ccaactggcc ggcgctcagc atcagcgagc ggccgagccg
     3541 cttgcgctcg cgcacctgcg acgcggggat catctgcgtg acgccgccgg tgctctgcac
     3601 gatcagtgga ttgccgccgc tcagcacgag cccatggatc ttgcggccgt cctgcaacgt
     3661 gatctccgtg ccctcgtagc catgcgcgat gtcgctggac ggagtgacga tggcggtgat
     3721 gacgacgtcg cgcgtctggc ggctcgcgaa accgtcgagc gccggtgcgt aatccacgcc
     3781 tttgccctgg atgcgatggc acatgaggca cgcgcccgcg gcggtggcgc cgcgcttcgc
     3841 gtcgccgcgt agtttggcga tgtctgcgac tgctggcagc gtggacttct cgtccggcgc
     3901 aggcaccgtg acctcgttga tcgccaccgt gtccggatcg tagagaccgc ggcgcttgag
     3961 ctccgcgtcg ataccctgcc ccgcccagcg cgaatccttg tagttcatga gccaccacag
     4021 cgcggagttt tgtttgatgt cactgccgcc gtgctgggcc acatcgagca atgccgtggc
     4081 ggcttcgctc gacgggatga atccgagcgc ggtcgtcgcc ttgctgcgct ccgatacgga
     4141 caaaacatcc gcgccggcgc gcgcggcgaa cgcgggcacg gccgcgcgtg gtgtcaggcg
     4201 ccacacgaga tccgcataag tcgccggcca tttcgtggca tcgctgtcgg gctgtgaggc
     4261 cgccagcgcc gtgtacagag cgtcttcctt gccggtcgca ccgatgcccc aggcttccag
     4321 gtagctgcgg tccttgccgt cgtacccttt ggccagcgcg ag
//
LOCUS       DATDEU010000265         3942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_46665, whole genome shotgun sequence.
ACCESSION   DATDEU010000265 DATDEU010000000
VERSION     DATDEU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46665"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VIV63_06475"
     CDS             <1..262
                     /locus_tag="VIV63_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124277.1"
                     /translation="GAGGAVAVELAAARPKVVRKVCLLGSPGKEANATAPGAEDLQDA
                     ASDWPADRRKLLKQPVLSLENETLDGSVKALTGKLAEFLSAQ"
     gene            complement(256..999)
                     /locus_tag="VIV63_06480"
     CDS             complement(256..999)
                     /locus_tag="VIV63_06480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY6124278.1"
                     /translation="MKTPPEPQRFNEFGQPIGFDLPGWKPPPFPPHVALQGRYCRIEP
                     LNAEKHARDIYEAQLEDTDGARWTYLFHGPYDDFAEYEKWAVGAQASRDPQFYALVDL
                     SSGRAVGTCAYMRIEPKHGVIEIGNIYFSPRLGRTRAATEAMYLLMANAFDLGYRRYE
                     WKCDSCNLPSRAAATRFGFTYEGLFRQAIVNKGRNRDTTWYAIIDRDWNAGLRDAYLR
                     WLDPANFDAGGAQKRKLSELTAPFVDQAH"
     gene            1138..3186
                     /locus_tag="VIV63_06485"
     CDS             1138..3186
                     /locus_tag="VIV63_06485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY6124279.1"
                     /translation="MLRSTILLTTLFCSTWVCAQTPAPDNSRVLTAEKMWALKRLGDP
                     AITPDGLTAVVPVTTYDIKENKGLTDLWLIPVAGGAARQLTADKASDTQPTVSPDGKW
                     IAFVSKRGDDTENQVYVIAVDGGEARRVTNVPTGVSVPKWFPDSSRLAFVSDIWPDLV
                     RWEDQGARKKERADSKMTARVWNRSPISYFDHYLDDREPHLFSIALEGGEPAAITRMS
                     GFHLSKLEIDASSYDISPDGLEVAFAADTDTTGIDSNYDLILIETCGCKPARNISAAN
                     KADEGYPRYSPDGRRLAFTQKRTMRFYADRDRLMIFDRAAGTTVGVTENWDRSVDGLV
                     WERDSRTLLGAIDDAATRRVYRFRLDGGVPTAITKASSFGSLALSRNGKALVGLRQSF
                     TEPPTLVAIAPRGGAATKLSTFNDAALAGFDQGKVESVVYKGAKDADIQMWVVYPPGF
                     DAAKKYPAMMLLHGGPHSAITDAVQWRWNAEVFASWGYVVTWHNFHGSSGFGNDFTDS
                     INPDQISMPYEDTIKAAEWLMAKPFIDKERMVAAGGSYGGFLATTLLGRKHPFKAMVA
                     HAAVFNRFTQLGADYGAETERFFNFWDKPEEFARLSPHTSAGNFNTPTLVIHNQNDLR
                     VPLNHGIELFNILQKRGVPSKLVYFPDENHWVLKPQNSLFWYQTVRDWVATYAKPGPY
                     "
     gene            3201..>3942
                     /locus_tag="VIV63_06490"
     CDS             3201..>3942
                     /locus_tag="VIV63_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6124280.1"
                     /translation="MKRRTLLTLAAAGAATLALPSWAAKKPKPLKILVLGGTRFIGLH
                     MTALALKRGHTLTFFNRGKTKTDRYPEIERIKGDRNGEIDGLKDREWDAVIDNSGYVP
                     RHARLSAELLAPKVKQYVFVSSISVYPNFSVARDESSPVGKLPDETVEKVDGESYGPL
                     KALCEQAVEKALPGRTTIIRPGLIVGPDDNTDRFTYWPARAARGGEFIAPGAPSDPFQ
                     VIDVRDLAAFTINAIENGTTGTYNLVSNV"
BASE COUNT          749 a         1255 c         1259 g          679 t
ORIGIN      
        1 tggcgcgggc ggcgcggtgg ccgtcgagct cgccgccgcc cggccgaaag tggttcgcaa
       61 ggtttgcctg ctggggtcgc ccggcaagga ggccaatgcc acggcgcccg gcgccgaaga
      121 ccttcaggat gcggcttccg actggcctgc cgatcgccgg aaactgctca agcaaccggt
      181 gctgtccctc gagaacgaga ccctcgacgg ctcggtgaag gcgctcaccg gcaagctcgc
      241 ggagttcctg tccgctcagt gagcctggtc gacgaacggc gccgtgagtt cggagagctt
      301 gcgcttctgc gcgccgccgg catcgaaatt cgcggggtcg agccaacgta gataggcgtc
      361 ccggagcccg gcgttccagt cgcggtcgat gatggcgtac cacgtggtgt cgcggttgcg
      421 gcccttgttg acgatggcct ggcggaacag gccttcatac gtgaagccga aacgcgtggc
      481 cgcggctcgt gatggcaggt tgcagctatc gcacttccat tcgtagcggc gatatcccag
      541 atcgaaggca ttcgccatca gcagatacat cgcctcggtg gccgcgcgcg tacgcccgag
      601 gcgcggcgag aagtagatgt tgccgatctc gatgacgccg tgtttcggtt cgatgcgcat
      661 gtaagcgcag gtccccaccg cgcgcccact cgaaaggtcg acgagcgcgt agaactgcgg
      721 atcacgcgag gcttgcgcac ccactgccca tttttcatat tcggcgaagt cgtcgtacgg
      781 cccgtgaaac agataagtcc agcgcgcgcc atcggtgtct tcgagctgcg cttcgtagat
      841 atcgcgagca tgtttttcgg cgttcagtgg ctcgatgcgg caatagcggc cctgcaaggc
      901 gacgtgcggc ggaaacggcg gcggcttcca gccgggcaga tcgaaaccga tgggctggcc
      961 gaattcgttg aagcgctgcg gctctggcgg ggttttcatg cccgcgattc tcgcatcgct
     1021 ccgggaccaa acgaagggac actacggcga gacgagcgcg aaaagcttgc ctcgacaaat
     1081 cgaatacgca gaatggcgca aacgcgcgat tccgcgcaaa caagaatcag gaaatccatg
     1141 cttcgttcca cgatcctgct caccacgttg ttctgctcca cctgggtttg cgcgcagacg
     1201 cccgcgcctg acaattcccg cgtgctcacc gccgaaaaga tgtgggcgct caagcggctg
     1261 ggcgatccgg ccatcacgcc ggatggcctc accgccgtgg tgccggtcac cacgtacgac
     1321 atcaaggaga acaagggcct cacggatctc tggctgattc ccgtggccgg cggcgccgcg
     1381 cgccagctca ccgccgacaa ggccagcgac acgcagccga cggtgagccc ggacggcaag
     1441 tggattgcgt tcgtctccaa gcgcggcgac gacaccgaaa accaggtcta tgtcatcgcg
     1501 gtcgatggcg gcgaagcccg gcgcgtcacc aacgttccga ccggcgtgtc ggtacccaag
     1561 tggtttccgg acagcagccg cctcgccttt gtgagcgaca tctggccgga tctcgtgcgt
     1621 tgggaagacc agggcgcgcg caagaaggag cgcgcggact cgaagatgac cgcgcgcgtc
     1681 tggaaccgtt cacccatctc ctatttcgat cactatctgg acgatcgcga accgcatctg
     1741 ttttccattg cgctggaagg cggcgagccg gcagccatca cccgcatgtc cggcttccac
     1801 ctgtcgaagc tggaaatcga cgcgtcgtcc tatgacattt ctccggatgg gctggaggtg
     1861 gctttcgccg ccgacaccga caccaccggc atcgacagca actacgatct catcctgatc
     1921 gagacctgcg gctgcaagcc ggcacgcaac atctccgcgg ccaacaaggc ggacgagggt
     1981 tatccgcggt acagcccgga tgggcggcgg ctcgcgttca cgcagaaacg cacgatgcgg
     2041 ttctacgccg atcgcgatcg cctcatgatc tttgatcgcg ccgcgggaac caccgttggc
     2101 gtcaccgaga actgggaccg ttccgtggac ggcctcgtgt gggaacgcga ctcgcgcacc
     2161 ttgttaggcg ccattgacga tgccgccacg cgacgcgtgt accgcttccg gctcgatggc
     2221 ggcgtgccca cggccatcac caaggcttcg agcttcggat cgctggccct gtcgcgcaac
     2281 ggcaaggcgc tggtgggtct ccgccagagt ttcaccgaac cgccgacgct cgtggcgatc
     2341 gcgccgcgcg gcggtgcggc gaccaagctg tcgacgttca acgacgcggc gctggcggga
     2401 ttcgaccagg gcaaggtcga gagtgtcgtc tacaagggcg cgaaggatgc cgacatccag
     2461 atgtgggtcg tctatccacc gggtttcgac gcggccaaaa aatatcccgc gatgatgttg
     2521 ctgcacggcg gcccgcatag cgccatcacc gatgcggtgc agtggcgctg gaatgcggag
     2581 gtgttcgcga gctggggcta cgtcgtgacg tggcacaact tccacggctc gagcggcttc
     2641 ggcaacgact tcacggattc catcaacccc gaccagatct ccatgccgta cgaagacacc
     2701 atcaaggccg ctgaatggct gatggcgaag ccgttcatcg acaaggagcg catggtggcc
     2761 gccggcggca gctacggcgg attcctcgcg accacgttgt taggccggaa acatccattc
     2821 aaggccatgg tcgcgcacgc cgcggtgttc aaccggttca cgcaactggg cgcggactac
     2881 ggcgcggaaa ccgagcggtt cttcaatttc tgggacaagc ctgaagagtt cgcgcgtttg
     2941 tcaccgcaca cgtcggccgg aaacttcaac acaccgacgc tggtcattca caaccagaac
     3001 gacctgcgcg tgccgctgaa ccatggcatc gaactgttca atatcctgca gaagcgcggc
     3061 gtgccctcga agctggtgta tttccccgac gagaaccact gggtgctgaa gccgcagaac
     3121 tcgctgttct ggtatcagac cgtgcgcgac tgggttgcaa cttatgccaa gcccggcccg
     3181 tattgatcga ggagacgccc atgaaaagac gaacactgct gacactcgcc gcggccggcg
     3241 cggccacgct ggcgctgccg tcctgggcgg cgaagaagcc gaagccgctc aagatcctgg
     3301 ttctgggcgg cacccgcttc atcgggctgc acatgaccgc gctcgcgttg aagcgcggcc
     3361 acacgctcac gttcttcaac cgcggcaaga ccaagacaga ccgctatccc gagatcgaac
     3421 gcatcaaggg cgatcgcaat ggcgagatcg acggcctgaa ggatcgcgag tgggacgcgg
     3481 tcatcgacaa ctccggctat gtgccgcgcc atgcgcggtt gtccgcggag ttgctggcgc
     3541 cgaaagtgaa gcagtacgtg ttcgtgtcgt cgatctcggt gtacccgaac ttcagtgtgg
     3601 cgcgtgacga atcgtcgccg gtcggaaaac tccccgacga gaccgtcgaa aaagtcgatg
     3661 gtgagagtta cgggccgctg aaggcgctgt gcgagcaggc cgtcgagaag gcattgcccg
     3721 gccgcaccac gatcatccgg ccggggctca tcgtcgggcc cgacgacaac acggatcgct
     3781 tcacgtattg gccggcgcgt gcggcgcggg gtggtgaatt catcgcaccg ggcgcgccca
     3841 gcgatccgtt ccaggtgatc gacgttcgcg accttgcggc attcacgatc aacgccatcg
     3901 agaatgggac taccggcact tacaacctcg tgtcgaacgt ca
//
LOCUS       DATDEU010000266         7760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_630876, whole genome shotgun sequence.
ACCESSION   DATDEU010000266 DATDEU010000000
VERSION     DATDEU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630876"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2132)
                     /locus_tag="VIV63_06495"
     CDS             complement(<1..2132)
                     /locus_tag="VIV63_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6124281.1"
                     /translation="MNIKRRLLGFGSSGMTCALAGFAIAASAQVSAQEAAPPPEEMEE
                     VVVSGIRASMRDSLGIKRESIQVVDAISAEDVGDFPDKNIGEAIQRVPGVQINRQDGE
                     GRSVSIRGADQSLIRVELNGVSALSLTVGGGRDVDFRDLPVEFVSRVEVVKTPTPEMT
                     EGGIGTVRVVTRHPFDQPEGFVAGSMQGVYSDLADAYDPKIALIGSKIFLDGTLGVLL
                     STQYETRHIDSNNARTTGWLRRAPTATGAGATPGRGTDVNADGTLDWVPEIPRYIIDR
                     RETTRQAFNAVVDWRPTDDFSLYLDGTYAKGREEVSSMLMQLGASGGLIDYANSSVGA
                     DNTVDHIELTSSAAFPMDLAFRNINGQLEREQYNGILGADWTVGNWKFDGRVSYARGE
                     VQNDEKNSTATIFGVPRAIIDYTGGEGAPNFSFPGIDTTDGALVNNVAAVFNPRTNDQ
                     QEEGARFNVEFKPELDWLTSVKAGVEKRDVTMESLLYARTIQISSRTPTPASAGATTT
                     VAVGQSVLAGIVNDNSGTNDVPFFDTGDLGFNGGIKYWNDNGDATYDATIAASGLTMD
                     PYGANANPGTNGTFQNYLDTWSVEEETLAGYLQAALAWDNAVVPVSALLGVRYYDTNT
                     LSEGYNRVQQGTVITFPTASQAGGYNDWLPSINVRFDFTEKFVGRMTAGEVMARPNPS
                     QLAFRRSTDIVGLTGSRGNPDLLPFKAN"
     gene            2603..3805
                     /locus_tag="VIV63_06500"
     CDS             2603..3805
                     /locus_tag="VIV63_06500"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HEY6124282.1"
                     /translation="MTGHRVVRLSTEAGTRSMYFHQNSVTPDGRYVITEGTAGIIAIE
                     IATRKNTLIVPGKVRALFVGRKTGLVYFSQSEGAGNSEQRTPTTISTIAATGGTPRRI
                     AQIERGFIGSVNADETLLLGVYAERAYQLEQGPRDPRFDANYAATGKDGKPLTFAEAK
                     EVRLLEREQARIPMAMFTVNTATGAQKIIHRSTDWLNHLQFSPTDPQELIFCHEGPWH
                     RVDRIWAMKLGGKPRIVHRRTMNMEIAGHEFFDVDGKTVWYDLQTPRGEVFWLASAAP
                     DGTRRWYHVDRDHWSVHFQISPDGKTFAGDGGDEEMVAHAKDGKWLYSFTPEAIPDVA
                     GISAPNAGDLVHPGTLRAERLVDMKKHDYRMEPNIIFTPDGRWLIFRSNMHGDVHTYM
                     VELAKATP"
     gene            complement(3806..5167)
                     /locus_tag="VIV63_06505"
     CDS             complement(3806..5167)
                     /locus_tag="VIV63_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY6124283.1"
                     /translation="MSALIAVFDVGKTNAKLAVVDPALGQEIWSTRRANNLVEGPGGR
                     ELDIVAIEAWLLESLREAPQRERIGVIVPIAHGAAAVLVDHEGVVLAAPDYEDPRFDE
                     VSAEYEPLRDNYSLTFSPGLPQGLNLGRQLFYLQTRRAEMFQRAAHLLLYPQYWSWRL
                     SGTMAAEVTSLGTHTDLWRPHENTYSTLARKQGWARMMPPQHRANARLGRIRPEIAAA
                     TGLDPTCEVACGIHDSNASYLRFLMDREREAFTVVSSGTWTIVMANRGDLRRLREDRD
                     MLANVNAFGAPVATARYMGGREYEAIAQSADEPTVDGVIEVLSQGSIALPSFASAGPY
                     SGRRGRLEIVDTLMGSQRAALATLYSALMTAVLIESLGTAGEIFVDGPLARNPLFARL
                     LAACVPVGTVRTYPEGGGTRVAAHLAGLPSPAAGALTSVAPLRLPGLLEYQANWRHRL
                     EER"
     gene            complement(5164..6114)
                     /locus_tag="VIV63_06510"
     CDS             complement(5164..6114)
                     /locus_tag="VIV63_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6124284.1"
                     /translation="MKPANLLRWESMLAAVLVLEFVVFSTISPYFLSVDTLSDATFNF
                     TERAIVALPLALLIIAGEIDISVAAIIALASVAMGLASAHGMDTAGITLVGLGTGLLA
                     GCINGALVTLGRVPSIVATIGTMTLFRGAAYAVLGDRVLKDYPESFAAFGQGYLAGPV
                     SIELALFALFAILFGVFLHRTRWGRTIFAIGANPVASRFAGVAVNQYRFALFALTGLF
                     SGLAAVLLTSRLGSTRPSIAQGWELEIISMVILGGVSVWGGKGSILGVVLAAVVFGLV
                     TFGLGLMNIPGIVMSIFTGTLLILVVAVPALVNRSPGKRP"
     gene            complement(6111..7076)
                     /locus_tag="VIV63_06515"
     CDS             complement(6111..7076)
                     /locus_tag="VIV63_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6124285.1"
                     /translation="MNSQSRRDLAFAALTLALFLAVTFAFPGFAAPRNLAGLLDDTAI
                     LIMLALGQMLVILVRGIDLSIAANLALCGMIAAVCDRAWPGMGVAPVLVLTLVSGALL
                     GAFNGLLVWKLRLPPIVVTLGTMSIYRGAIYLLSKGAWVNENEMSREFLAFPRAGFLG
                     LSLLSWMALIVAGVFIYALRHSRVARDLYAAGGNPEAANYSGIDAGRMQFIAYTVCGA
                     IAGVCGYLWVARFAVAYTDIALGFELSVIAACLIGGVAIAGGVGSAVGVVLGCLFIGI
                     IRSSLPLVGISAFWQMFINGVVILVAVLLSARQREFKRTILEVRA"
     gene            complement(7073..>7760)
                     /locus_tag="VIV63_06520"
     CDS             complement(7073..>7760)
                     /locus_tag="VIV63_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6124286.1"
                     /translation="PGAELLRVENLSRAGEFEGISFALRSGEILGIYGLVGAGRTELA
                     EVLFGINAADSGSVTSSADIALVPEDRQSQGSILPFSVAANIALPNLAALAPRGWCDS
                     TVEAALARQWIDRLAIKAHGPDQPVQNLSGGNQQKVVIAKWLARRPGVLILDEPTKGI
                     DVGAKAAVHAVTSEFTRDGGGVLMISSDLPEILGMSDRVLVMRRGRLRGEFTRAEATG
                     ENLLRAASDA"
BASE COUNT         1476 a         2499 c         2483 g         1302 t
ORIGIN      
        1 tgattggcct tgaacggcag cagatccgga ttgccgcgcg aaccggtgag accgacgatg
       61 tcggtcgagc gccggaatgc gagctgagac ggattcggcc gggccatcac ttcgcccgcc
      121 gtcatgcggc cgacgaattt ctcggtgaag tcgaaacgca cgttgatgga aggcagccag
      181 tcgttgtatc cgccagcctg agaggccgtc gggaacgtga tgaccgtgcc ctgctgaacg
      241 cggttgtagc cttccgacag cgtgttcgtg tcgtagtagc gcacgcctaa caacgcgctg
      301 accggcacga cggcgttgtc ccaggcgagc gcagcctgca gatagcccgc cagtgtctct
      361 tcctcgaccg accacgtatc cagatagttc tggaacgtgc cattggttcc cggattagca
      421 ttggcgccgt aaggatccat cgtgagaccg ctggcggcga tggtggcgtc gtaggtcgcg
      481 tcgccattgt cgttccagta cttgatgccg ccgttgaaac ccaggtcgcc ggtgtcgaag
      541 aacggcacgt cgttggttcc cgagttgtca ttgacgattc cggcgagcac ggactggccc
      601 acggccacgg tcgtggtggc gccagcgctg gccggtgtcg gggtgcggct gctgatctgg
      661 atggtgcgcg cgtagagcag gctctccatg gtgacatcgc gcttctcgac gcccgccttg
      721 acggacgtca gccagtcgag ctcgggcttg aactccacat tgaagcgcgc gccctcttcc
      781 tgctggtcat tggtgcgcgg gttgaacacc gcggcgacgt tgttcaccag cgcgccgtcg
      841 gtggtgtcga tcccggggaa agagaagttg ggagcgcctt cgccgcccgt gtagtcgatg
      901 atggcgcgag gcacgccgaa gattgtcgcg gtggaattct tttcgtcgtt ctggacttcg
      961 ccgcgcgcat agctgacacg gccgtcgaac ttccagtttc ccaccgtcca gtccgcgccg
     1021 aggatgccgt tgtactgctc gcgctcgagc tggccgttga tgttgcggaa cgccaggtcc
     1081 atcggaaaag ccgcgctgct ggtcaattcg atatggtcca cggtgttgtc cgcgccaacc
     1141 gaagaattcg cgtagtcgat gagaccgccg gaggcgccga gctgcatgag catgctgctc
     1201 acttcctcgc ggcctttcgc atacgtgccg tcgagataca gcgagaagtc gtcggtcgga
     1261 cgccagtcga ctaccgcgtt gaacgcctga cgcgtggtct ctcgccgatc gatgatgtag
     1321 cgaggaattt ccggaaccca gtcgagcgtt ccatccgcgt tgacgtcggt gccacggccc
     1381 ggggtcgcgc ccgcgccggt tgccgtgggg gcgcgacgca gccagcccgt ggtgcgcgcg
     1441 ttgttgctgt cgatgtggcg ggtttcgtac tgcgtggaca gcaacacgcc gagcgtgccg
     1501 tccaggaaaa tcttgctgcc gatcagcgcg atcttcggat cgtaggcatc ggcgagatcg
     1561 ctgtagacgc cctgcatcga gccggcgaca aagccttccg gctgatcgaa gggatgacgc
     1621 gtcacgacgc gtaccgtgcc gataccgcct tcggtcatct ccggagtcgg cgtcttcacg
     1681 acttcgacgc gggacacgaa ttccacaggc agatcgcgga agtccacatc gcggccgcca
     1741 ccgacggtca gcgacaacgc ggagacaccg ttgagctcga cacggatcaa cgactggtcc
     1801 gcgccacgaa tgctgacgct gcggccttcg ccgtcctgcc ggttgatctg gacgcccggc
     1861 acgcgctgga tcgcttcgcc gatgttcttg tcgggaaagt caccgacgtc ttcggcggaa
     1921 atggcgtcga cgacctggat cgattcgcgc ttgattccga gtgagtcgcg catcgaggca
     1981 cgaatgccgc tgacgacgac ctcttccatt tcctcgggcg ggggcgcggc ctcctgagcc
     2041 gaaacctgcg cgctcgcagc gatcgcgaaa cctgccagtg cgcaggtcat tcccgacgaa
     2101 ccgaaaccca ataaacgacg cttgatgttc accacagctg gccccccata gtggttgatg
     2161 gacgcaggcg tgttagcgcg gcgacgtccg ttgttgaatc agactgtgcg cgaatttgct
     2221 cctgattgat cgctattgca aatttttgtc tggaggattc cctgactgcg gatgtgagcg
     2281 gtcacattcg cggctggctg ttgccacaaa cccacgaccc gccgcgacgc ggattcctac
     2341 attttcaagg ccactcacgc gtgtctggcc gaagccctgt gagttttctg tcgcaagtga
     2401 cgccagaacg ccgactttgc cgtatcgatc aggattgtgc atattcgctc accgcacgcc
     2461 gcccggcgtg ttagtcgagg ggaccgacat gcgaacgatt gggcaggctg cgtcgtgggg
     2521 tttgatcgcg ctcctgggaa gcatccgcgc tgattccgcg gaacccctga aggcagctcc
     2581 gccagccgag tggatagaac ccatgaccgg ccaccgcgtg gtccgcctgt cgaccgaggc
     2641 cggaacccgg tcgatgtact ttcaccagaa cagcgtcacg cccgacggtc gatacgtgat
     2701 caccgaaggc acggcgggga tcatcgccat cgagatcgcg acacgaaaga acaccctgat
     2761 cgtcccgggc aaggttcgcg ctctgttcgt cggccgcaaa accggcctgg tgtatttctc
     2821 gcagagtgag ggcgccggaa attccgagca gcgcacaccc accaccattt ccacgatcgc
     2881 ggcaaccggc ggaacgccgc ggcgcattgc gcaaatcgag cgggggttca tcggttcggt
     2941 caacgccgac gaaacgctgt tgttaggtgt gtacgccgaa agggcgtatc aactcgagca
     3001 agggccgcgc gatccgcgat tcgacgccaa ctacgcggcg accggcaagg acggcaagcc
     3061 attgacgttc gccgaggcga aggaagtgcg tctgctcgag cgcgagcagg cgcgcatacc
     3121 gatggccatg ttcaccgtga acactgctac cggcgcacag aagatcattc accgctccac
     3181 ggactggctg aaccatctgc agttctcgcc cacggatccg caagagctca tcttttgtca
     3241 tgaaggaccg tggcaccgcg tcgatcgcat ctgggcgatg aagctcggcg gcaagccgcg
     3301 catcgttcat cggcggacca tgaacatgga aatcgccggc catgagttct tcgacgtcga
     3361 cgggaagacc gtctggtacg acctgcagac gccgcgcggt gaggtgttct ggctggcttc
     3421 ggccgcgccc gatggcacac ggcgctggta ccacgtcgac cgcgaccatt ggtcggtgca
     3481 tttccagatc tcgccggacg gcaagacttt cgccggcgac ggcggcgacg aggaaatggt
     3541 cgcgcacgcg aaggacggaa aatggctgta ttcattcacg ccggaggcga tccccgacgt
     3601 ggcgggaatc tcagcgccga acgccggaga tctcgtgcat ccgggaacgc tgcgcgccga
     3661 gcgcctggtc gacatgaaga aacacgacta ccggatggag cccaacatca tcttcacgcc
     3721 cgacggccgg tggctcattt tccgctcgaa catgcacggt gacgtgcaca cgtacatggt
     3781 ggaactggcg aaggcgacgc cctgactacc gctcctcgag gcgatgccgc cagttggcct
     3841 ggtattcgag cagacccggc aggcgaagtg gcgccacgga agtcagcgcg ccggccgcgg
     3901 gtgaaggcag gcccgccagg tgcgcggcga cgcgcgtgcc gccgccctcg ggatacgtgc
     3961 gcacggtgcc gaccggcacg cacgcggcta acaatcttgc gaacaatgga tttcgcgcga
     4021 gcgggccgtc gacgaatatt tcgccggccg tgcccagcga ttcgatgagc accgcggtca
     4081 tcagcgcgga atagagcgtg gccagcgcgg ctcgttgtga gcccatcaat gtatcgacaa
     4141 tttccagacg cccgcgccgg ccggagtacg gccccgcgct cgcgaatgac ggcagcgcga
     4201 tcgatccctg cgacagcact tcgatcaccc catccacggt gggctcgtcg gcgctctgcg
     4261 caatggcttc gtattcgcgc ccgcccatat aacgcgccgt ggcaaccggg gcgccgaacg
     4321 cgttgacgtt ggccagcatg tcgcggtctt cacgcagacg ccgcaggtcg ccgcgattcg
     4381 ccatgacgat ggtccaggtg ccactcgaca ccaccgtgaa cgcctcgcgc tcgcggtcca
     4441 tgaggaatcg caggtaggaa gcattggaat cgtggatacc gcaggccact tcgcacgtgg
     4501 gatcgagccc ggtcgcagcg gcgatctcgg gcctgatgcg ccccagccgc gcgttcgccc
     4561 ggtgttgcgg cggcatcatg cgtgcccatc cctgcttgcg tgccagcgtg gaataggtgt
     4621 tctcgtgcgg acgccacagg tccgtatgcg tcccgagcga ggtcacttcc gccgccatcg
     4681 tccccgacag gcgccatgac cagtactgcg ggtagaggag cagatgcgcg gcgcgctgga
     4741 acatttcagc gcggcgcgtc tgcagataga agagctggcg gccgagattg agtccctgcg
     4801 gaagacccgg cgaaaaagtc agtgaatagt tatcgcgcag cggctcgtat tccgcgctca
     4861 cttcatcgaa gcgcggatct tcgtagtcgg gcgccgccag cacaacgcct tcgtgatcga
     4921 ccagaacggc tgccgcgccg tgcgcgatgg gcacgatgac gccgatacgt tcgcgctgcg
     4981 gagcttcgcg cagggactcg agaagccagg cttcgatcgc gacgatatcg agctcacgtc
     5041 cgcccgggcc ttcgacgaga ttgttagccc gccgggtgct ccagatttcc tgtcccagcg
     5101 cgggatcgac cacggcgagc ttcgcgttgg tcttgccgac gtcgaaaaca gcgatgagcg
     5161 cgctcatggt cttttcccag gggagcgatt caccaaagcc ggcacggcga ccacgaggat
     5221 gagcaacgtg ccggtgaaga tcgacatcac gatgccggga atgttcatga gtccgagtcc
     5281 gaaggtcacc agtccgaaca ccaccgcggc gagcaccaca cccaggatgc tgcccttgcc
     5341 gccccacacc gatacgccac ccaggatgac catgctgatg atctccaact cccatccctg
     5401 cgcgatcgaa ggccgcgtcg accccaggcg cgatgtcagc agcacggcgg cgagtccgct
     5461 gaacaatccg gtgagtgcga acaaggcgaa acgatattga ttcaccgcga cacccgcaaa
     5521 ccggctcgcc accgggttgg cgccgattgc gaatatcgtg cggccccagc gagtgcgatg
     5581 caggaacacg ccgaacaata ttgcgaagag cgcgaacaac gccagctcga tggacacggg
     5641 cccggccagg tagccctggc cgaatgccgc gaaactttcc gggtaatcct tgagcacgcg
     5701 atcgcccaga accgcgtatg ccgcgccgcg aaacagcgtc atggtgccga tggtggcgac
     5761 gatggaaggt acgcggccga gggtcacgag ggcgccattg atgcagccgg cgagcagccc
     5821 ggtgcccagg ccaacgagcg ttattccggc cgtgtccatc ccgtgcgcgg acgccagacc
     5881 catggccacg gacgcaagcg cgatgatggc cgccaccgag atatcgattt cgccagcgat
     5941 gatcagcagc gccagtggca gcgccacgat ggccctttcg gtgaaattga aggtggcgtc
     6001 cgaaagcgtg tcgacactca ggaaatacgg cgagatggtc gagaacacca cgaactcgag
     6061 caccagcacc gcggccagca tcgattccca gcgaagcagg ttcgccggct tcaagcgcgc
     6121 acctcgagga tggtgcgctt gaactcgcgt tgccgcgcgc tcaacaacac cgcgacaaga
     6181 atgacgacgc cattgatgaa catctgccag aacgcggaga tgcccacgag cggcaagctc
     6241 gagcgaatga tcccgatgaa caggcatccc agcaccacgc ccacggccga gcccacacca
     6301 ccggcaatcg cgacgccgcc gatcaggcag gcggcaatca ccgagagctc gaagcccagc
     6361 gcgatatcgg tgtaggcgac cgcgaagcgc gccacccaca ggtatccgca cacgccggcg
     6421 atggcgccgc agacggtgta ggcgatgaac tgcatgcgcc cggcatcgat gcccgagtag
     6481 tttgccgcct cggggttccc gcccgccgcg tagagatcgc gtgccacgcg cgagtggcgc
     6541 aacgcgtaga tgaacacccc ggcgacgatc agcgccatcc aggagagcag cgacaggccc
     6601 aggaacccgg cgcgcgggaa tgcgaggaac tctcgcgaca tctcgttctc attgacccag
     6661 gcgcctttcg acagcaggta gatggcgccg cgatagatgg acatggttcc gagcgtcacg
     6721 acgatgggcg gcagccgcag tttccacacc agcaggccgt tgaaagcgcc taacaaggcg
     6781 ccggatacca gcgtgaggac gagaaccggc gccacaccca tgccgggcca ggcgcggtcg
     6841 catacggccg cgatcattcc gcacagcgcg agattcgccg cgatcgacag atcgatgccg
     6901 cgcacgagga tgaccagcat ctggcccagc gccagcatga tgaggattgc cgtgtcgtcg
     6961 agcaggccgg cgagatttcg cggcgccgcg aatccgggga acgcgaacgt gacggcgaga
     7021 aacagcgcca gtgtgagcgc ggcaaatgcc aggtcgcggc gcgattggga gttcatgcgt
     7081 cgctcgcggc gcgcaggaga ttctcgccgg tcgcctcggc gcgtgtgaac tcgccgcgta
     7141 aacgcccgcg ccgcatcacc agtacccgat ccgacatgcc gaggatttcc ggcaggtcgg
     7201 acgagatcat caggacgccg ccaccatcgc gcgtgaattc gctggtcacg gcatgcacag
     7261 ccgctttcgc gcccacgtcg atacccttgg tcggttcatc gaggatcagc acgccgggcc
     7321 gccgggccag ccacttggcg atcacgacct tctgctggtt gccaccggag aggttctgca
     7381 ccggttgatc ggggccgtgc gccttgattg ccagccggtc tatccactgc cttgcgagcg
     7441 cagcctcgac agtcgagtcg caccagccgc gcggcgcgag cgccgcgaga ttcggcaatg
     7501 caatgttcgc ggctaccgag aacggcagga tgctgccctg ggactggcgg tcttccggca
     7561 ccagcgcgat atccgcgctc gaggtgacgc tcccggaatc ggcggcgttg atgccgaaca
     7621 gcacctcggc gagttcagtt cgaccggcgc cgacgagacc gtagatgccg agaatctcgc
     7681 cgcttctcaa cgcgaaggaa atgccttcga actccccggc ccggctcagg ttttccacac
     7741 ggagcaactc ggcgccaggc
//
LOCUS       DATDEU010000267         4840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_923004, whole genome shotgun sequence.
ACCESSION   DATDEU010000267 DATDEU010000000
VERSION     DATDEU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923004"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VIV63_06525"
     CDS             <1..588
                     /locus_tag="VIV63_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY6124287.1"
                     /translation="EDPALVFSEAAVLLRPGAPSRMAEARELAATLEAEFPKVLQLSE
                     GFADGGDILVTPDGVLIGLSARTNDAGAADLQRLLESIGMKSKVVAVPRGTLHLKSDC
                     SLVDEETILATPELAGSGLLEGYRTLVVPDDERHATNAIRVNDVVFIRAGCPRTLELL
                     QRHGLNVLPLQATEIAKIDAGLSCMSLRWFGRLST"
     gene            complement(581..1981)
                     /locus_tag="VIV63_06530"
     CDS             complement(581..1981)
                     /locus_tag="VIV63_06530"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6124288.1"
                     /translation="MLLRVTSIAMFVFAAAAQAQTADTGKAAFRGIYQEMVEIDSSPT
                     TGSCTKVVRAAESRLQAAGFAPADIHVVIPDGKPDDGNIVAHIRAPGAKKKGVLLLAH
                     IDVVDAKRADWERDPFKLIEEDGYFYGRGTADDKAMAAVFLDLMIRLKAERGFKPKRD
                     LILALTCGEETSNRVNGVDYLLQNKRELIDAAFAINEGAGGLLAADGKKHLVLQVQAG
                     EKIHQVYSLEVTNKGGHSSRPERENAIYRLARATLAIENLAFPLEITPVVREYFRVTG
                     PTLGGEIGAAMSAVGKDPGDKAALAPLTNDPSYNAQLHTTCVATQVDAGHAPNALPQR
                     ATATLSCRVMQGTTPEQVKESIEKIVGDPQVKVEIVRRRSGSSAPELTDEIMKPVRAA
                     AAKLWPGVPIAPSMTAGATDGRFLMSAGIPTYGMSGMFGVSGENNAHGLNEKIRVTSL
                     YEGREFLESIVRAYVR"
     gene            complement(2054..2965)
                     /gene="rarD"
                     /locus_tag="VIV63_06535"
     CDS             complement(2054..2965)
                     /gene="rarD"
                     /locus_tag="VIV63_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEY6124289.1"
                     /translation="MARNSGASTASGGFTAAIAAFVIWGLFPLYLIGLLQVSAMQITA
                     HRIVWSCVFVVAWLAVRRELGGLWAAVTRDGGLIRLTARAFFIAVNWLGFVWAVGQHR
                     VLEVSLAYFMGPLLNILLGIFVLSERLDRTQWIAVGFAAAGVAYLTFIAGHAPWIALM
                     VGSSFALYGLIRKTVSIDALPGLAVETILLAPIAVGYLIWCEIQGTGALGHSGWRVDA
                     LLLAGGIVTSVPLFLFSYGARRVPFSTIGVLQFIAPTLQLIGGLVVFGEPFESARATG
                     FVLIWIGLSIYAGNALWRARSLKPAAT"
     gene            3077..4684
                     /locus_tag="VIV63_06540"
     CDS             3077..4684
                     /locus_tag="VIV63_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6124290.1"
                     /translation="MMAPALKLRLLGTLAVSQHGEAVDLPASRKARALVGFLAFASRA
                     TPRSRLCELLFETVGDPRGELRWYLSKVRGVVGATRVRTTENSVRIDLADSFVDAVEI
                     QDAARAGFANLAPEKARALLAMFDGDFLEGLDVDGCPGIAGWLLAQRRRFHAWRIALL
                     QRLVETAPESELFAHLEKWVELAPFDVRAHEQLMRALVRRGRFQEAKEHLATSMKLFL
                     AEDLDSAPLGKAWRTAKAEGATPALRPGDRECELAYDCFLQGRQHLARMMQRGLESSR
                     HMFVRAIELDANYGPAWAGLATVHSCLGEWFDAGSVSLAHAEQTSRRALEAAPHLAES
                     HVARGLARSLSKHHDEANREFEEAIRLDPYLFDAYYYYARTMFARGDLARAAELFESA
                     AHLRQEDFQSPMLCGMALRGLGREDAARDAVQMGIRRAERTLVLNPLDGRALSLGAGA
                     LVDDDQTERALEWSQKAYELYPDDTSALVNIACVYSRLDQRDEALALLEGVFSRGCGK
                     RDWVEHDPDYSNLRGEPRFHRLISGLR"
BASE COUNT          854 a         1524 c         1610 g          852 t
ORIGIN      
        1 gaggaccccg cgctggtatt cagcgaggcg gcggtgttgc tgcgtcccgg tgcgccctca
       61 cgcatggcgg aggcacggga actggcggcc acgctcgagg cggaattccc caaggtcttg
      121 caattgagcg agggcttcgc cgacggcggc gacatcctcg tcacgcccga tggcgtgctc
      181 atcggcctgt ccgcgcgaac caacgatgcg ggcgcggccg atctgcaacg cctgctcgag
      241 tcgattggca tgaagagcaa agtggtggcc gttccgcgcg gaacgctgca tctcaaatcc
      301 gactgctcgc tcgtggacga ggagacgatc ctcgcgacgc cggagcttgc gggttcaggg
      361 ctgctcgagg gatatcgcac gctggtcgtg cccgacgatg aacgccacgc gaccaatgcg
      421 atccgcgtca acgatgtggt gttcatcagg gcagggtgcc cgcgaacgct ggagcttctt
      481 cagcgacacg gactgaatgt attgccgttg caggcgaccg agatcgccaa aatcgatgcc
      541 gggctatcct gcatgtcgct gcgctggttc ggccggctat ctacctgacg taggcccgta
      601 cgatgctttc gagaaattcg cgtccttcgt acagcgacgt cacgcggatc ttctcgttca
      661 gcccgtgcgc attgttttcc ccggacacgc caaacatgcc gctcatgccg taggtcggga
      721 ttcccgcgct catgaggaat cggccgtccg tagcgcccgc cgtcatcgac ggcgcgatcg
      781 gaacaccggg ccacaacttc gcggccgcgg cccgcaccgg cttcatgatt tcgtcggtga
      841 gttccggcgc gctcgagcca ctgcgccggc gcacgatttc cactttcacc tgggggtcgc
      901 cgacgatctt ttcgatcgat tccttcacct gctcgggagt cgttccctgc atgacgcggc
      961 aggacagagt cgcagtcgcg cgttgtggca acgcgttcgg cgcgtgaccg gcatcgactt
     1021 gcgtggcgac gcaggtggtg tggagctggg cgttgtagct ggggtcgttc gtgagcgggg
     1081 ccaacgcagc tttgtcgccg ggatcttttc caaccgccga catcgcggcg ccgatctcgc
     1141 cacccagcgt cgggccggtg acacggaaat attcgcgcac gacgggcgtg atctcgagcg
     1201 ggaacgccag gttctctatc gcaagcgtcg cgcgtgccag tcgatagatg gcgttctcgc
     1261 gttccggccg cgacgaatgc ccgcccttgt tggtcacttc cagtgaatag acctggtgaa
     1321 tcttctcgcc ggcctgcacc tgcaacacga gatgtttctt gccgtcggca gcgagcagac
     1381 cgcctgcgcc ttcgttgatc gcaaatgccg cgtcgatgag ttcgcgtttg ttctgcagca
     1441 gatagtccac gccgttcacg cggttcgagg tttcttcgcc gcaggtgagc gccaggatga
     1501 gatcgcgctt cggcttgaaa ccgcgctcgg ccttcagccg aatcatgagg tcgagaaaca
     1561 ccgcggccat tgccttgtcg tcggccgtgc cgcgtccata gaagtagccg tcttcctcga
     1621 tcagcttgaa gggatcgcgc tcccagtcgg cgcgtttcgc atccaccacg tcgatgtgcg
     1681 cgagcagcag cactcctttt ttcttggcgc cgggcgcacg gatgtgcgcg acgatgttgc
     1741 cgtcatccgg cttgccgtcg ggaatcacga cgtggatgtc ggcaggtgcg aatcccgcgg
     1801 cctgcagtcg cgactcggcg gcgcgcacga ccttcgtgca ggaaccggtc gtcggtgacg
     1861 aatcgatctc gaccatttcc tggtagatcc cccgaaacgc tgccttgccg gtgtcggcgg
     1921 tctgcgcctg cgcagcggcg gcgaaaacga acatcgcgat ggacgtgact cggaggagca
     1981 tggggggtct cgatgaatgg cgtggaaggc gagcctccca caactcgggt gcggagacga
     2041 gacgcgggtg tcgttacgtc gcggcgggct tcagcgaacg cgcccgccac aaagcgttgc
     2101 cggcgtagat cgaaagcccg atccagatga gcacgaatcc ggtggcgcgc gcggattcga
     2161 acggctcccc gaataccacg aggccaccga tgagttgcaa agtcggggca atgaactgca
     2221 ggacgccaat cgtcgagaac ggaacccgtc gcgcgccata agaaaagagg aataacggta
     2281 cggaggtgac gatgccgccg gccaggagca gggcatcgac gcgccagccg gaatgtccca
     2341 gcgcgccggt tccctggatc tcgcaccaga tcaggtagcc gaccgcgatc ggcgcgagca
     2401 ggatcgtttc gaccgcgagg ccgggcagcg cgtcgatgct caccgtcttg cggatcaatc
     2461 cgtacagcgc aaaggaggag ccgaccatga gcgcgatcca tggagcatga ccggcgatga
     2521 acgtgagata ggcgacgccg gcggcggcga agccgacggc tatccactgc gttcgatcca
     2581 gccgctccga caacacgaag atgcccagca ggatgttgag cagcgggccc atgaagtacg
     2641 caaggctcac ttcgagcacg cggtgctgac ccacggccca cacgaagccg agccaattga
     2701 cggcgatgaa gaacgcgcgg gcggtcagtc gaatcaggcc gccgtcgcgc gtgacggccg
     2761 cccacagacc gccgagctct cgcctgacgg cgagccacgc gaccacgaac acgcaggacc
     2821 acacgatgcg atgcgcggtg atctgcatcg ccgagacctg caacaggcct attaaataga
     2881 gtgggaagag gccccagatg acgaacgcgg cgatcgccgc ggtaaacccg ccgctggccg
     2941 tggatgcccc ggagtttcgt gccatgcggc attgtgccga agtaccggcc accaggcgtc
     3001 taccggatga gggaccccgg cgaaatcccg gtgttgtccc gggactcgtg ggcgccgtca
     3061 ctcgtatcct cggttgatga tggcccccgc cctgaaattg cgcctgctcg gaacgctcgc
     3121 tgtctcgcag cacggcgagg cggtggactt gccagcctcg cgaaaggcac gagcgctcgt
     3181 cggctttctc gcgtttgcgt cgcgcgccac gccccgctcc cgcctttgcg aattgctttt
     3241 cgagaccgtg ggcgacccgc ggggtgagct gcgctggtat ctcagcaagg tgcgtggggt
     3301 cgttggagcc acccgcgtac gaactaccga aaattcagtg cgcatcgacc tcgcggacag
     3361 cttcgtcgac gcggtcgaaa tacaggacgc cgcgcgcgcg ggtttcgcga accttgcacc
     3421 cgagaaagcg cgggctttgc tggcgatgtt cgatggcgac ttcctcgaag ggctcgatgt
     3481 cgatgggtgc cctggcatcg cgggttggct gctcgcgcaa cggcggcgtt tccacgcgtg
     3541 gcgcatcgcg ttgcttcagc ggctcgtcga gaccgcaccg gaatccgagt tgttcgcgca
     3601 tctcgagaag tgggtcgagc tcgcgccgtt cgacgtgcgc gcgcacgaac aactgatgcg
     3661 cgcgctggtg cgtcgcggcc gcttccagga agccaaggag catctcgcca cttcgatgaa
     3721 gctttttctc gcggaggatc tcgacagcgc tccgctgggc aaagcctggc gcacggccaa
     3781 ggcggaagga gcaacgcccg ctctcaggcc gggcgatcgc gagtgcgaac tggcgtacga
     3841 ctgcttcctg cagggacggc aacacctcgc acgcatgatg cagcgagggc tggagtcgag
     3901 ccgtcacatg ttcgtccgcg cgatcgagct ggacgcaaac tacggacctg cctgggcggg
     3961 ccttgcgacg gtgcatagct gtctcggcga gtggttcgat gccggaagtg tttcactcgc
     4021 ccatgcggag caaacgagcc ggcgcgcact ggaagccgcg ccgcatctcg ccgagtcgca
     4081 tgtcgcgcga ggcctggcgc gttcgctgtc gaagcaccat gacgaggcca atcgcgaatt
     4141 cgaagaagcg atccggctcg acccctatct gttcgatgcg tattactact atgcgcgcac
     4201 gatgttcgcg cgtggcgacc tcgcacgggc ggccgagttg ttcgagtcgg cggcgcatct
     4261 gcgccaggaa gactttcaaa gccccatgct gtgcggcatg gcgttgcggg gtctgggacg
     4321 cgaagatgcc gcgcgcgacg ctgtccagat ggggatccgg cgcgccgagc ggacactcgt
     4381 gctgaatccg ctcgacggcc gcgcgttgtc actcggcgct ggagcgctcg tcgacgatga
     4441 ccagacggag cgcgctctcg aatggtcgca gaaggcgtat gaactctatc cggatgacac
     4501 cagtgcgctc gtgaacatcg cctgcgtgta ttcgaggctg gatcagcggg acgaagcgct
     4561 ggccctgctg gagggtgtgt tctcccgcgg atgcggaaaa cgcgattggg tggagcacga
     4621 tccggactat tccaatctgc gtggcgagcc gcgctttcat cgattgatca gcgggcttcg
     4681 atagcgccag gcgcgtcagg cacaccgctg cgcacgcacc agcaggagcg cgccgcgagg
     4741 ggaagtgatg gcggcatgca cggttccggc ttcagccacg tgcatatctc cggcgcacag
     4801 cgggtgagcg ccgatgaaga tctcgccttc gaggaccagg
//
LOCUS       DATDEU010000268         4758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_47641, whole genome shotgun sequence.
ACCESSION   DATDEU010000268 DATDEU010000000
VERSION     DATDEU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47641"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VIV63_06545"
     CDS             <1..1088
                     /locus_tag="VIV63_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6124291.1"
                     /translation="GHRVEVFEKSPGIIANGTGILLQPAGIDVLHGLDLADSAFERGC
                     AIERVVARSRGARRMDLNYADLRPGLCALGIRRPALAALLHDAARSAGAVFHFGVAVE
                     RLSEAPDHVSLLERGGSTAHGPFDIAVICNGLNSRLRDRVSPGAKVRPHSRGVFSVVA
                     PMPAGLSGEVLLQAMNGWRDGVGLLPIGRGDGATPLVSFFWNARESDRPRLETDGYPA
                     WCDYIDGFCPEAGGLLRSLSGFDALTWSTTADVALSRWHGARTLVMGDAALALNPQLG
                     LGATRALLDAQALSEVLRDGGVPAALPAYQARRRPQVSRYARVSRFWSRLDGSGFSPL
                     RRRLFLAAANGFPALRRRMLQHVCGYA"
     gene            complement(1094..3424)
                     /locus_tag="VIV63_06550"
     CDS             complement(1094..3424)
                     /locus_tag="VIV63_06550"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEY6124292.1"
                     /translation="MITRRQTLKGGIALGACATSAFRVAGAAEAGASPLTLWYQKPAE
                     QWTEALPIGNGRLGAMIFGGIARERLQINEDTLYAGGPYDPSNPDALLALPEVRALIA
                     AGKYAEAQDLAEQKMMARPIRMPSYQTVGDLILTFGASSYAQDYRRDLDLQSACAHVR
                     FQRDGVKFTRESFATAVDQVIVTRITADRPGALNFQAGFETPMPAAVAIDGAHLVLNG
                     RNVEQQGIAAALRFQARALVIPQGGQMQLRDGEFVVTGADSALVLVAAATSFRRFDDV
                     SGNPEAITRAQIQAAAGKSFDALHAAHVADHRKLFGRVSFELPATPAAEQPTDLRIRN
                     SQTSDDPQLAALYFQYGRYLLIGCSRPGTQPANLQGLWNDKLLAPWGSKYTININTEM
                     NYWPAEPANLAECVQPLVAMVKDLAVTGAITARVNYGAGGWVAHHNTDLWRASGPIDG
                     AKWGMWPTGGAWLCKHLWDHYDYGRDRAYLADVYPLLRGAAQFFLDTLVKDPSGKYLV
                     TSPSISPENLHPHGAAICAGPAMDSQILRDLFANTIHAAEILGKDAEFRAACAATRAK
                     LPPDRIGKAGQLQEWMEDWDMEAPERDHRHVSHLYALFPGEQITVRRTPELAAAVKRS
                     LELRGDLSTGWAIAWRINLWARLRDAEHTHGVIKLLLDPSRTYPNMFDAHPPFQIDGN
                     FGGANGMMEMLLQCVDGEIELLPALPRAWSTGSVRGLRARGGFELDMAWRDGELVSAQ
                     LRGAPGGVAQVRYREKVAKVRVAKGRSLTLTPAKFG"
     gene            3561..4382
                     /locus_tag="VIV63_06555"
     CDS             3561..4382
                     /locus_tag="VIV63_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124293.1"
                     /translation="MPYVTTDDHTQLYYKEWGRGRPVVLLHGWPLSADSWDDQAFALA
                     NAGYRVIAYDRRGFGRSSQPWNGYDYDTLTDDLRSVLKFCQVSEAALVGFSMGGGEVA
                     RYMSKYNGAGIAQAGLIASIVPYKLKTEDNPHGTELKALEETTAAIKEDRPRFLAEFF
                     KKFYGVDKAEKPVSDEVLQWSANVAMQAGLKATLACADSFFKTDFRPDLASFKVPTLI
                     IHGTADQNVSIDASARPAAQGIRNSKLIEYEGSPHGLLATDKQRVIDHLLAFLKG"
     gene            complement(4389..4727)
                     /locus_tag="VIV63_06560"
     CDS             complement(4389..4727)
                     /locus_tag="VIV63_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124294.1"
                     /translation="MKNIKLAFAAIILGLTSSLAVAAPTTYTFDTLTSIKMNITEPTL
                     SGVQRNATTPSTIYFIDQTNVSFRFVVNRCVPIFLTMMEKPGRYWLHVTVDPALSNVQ
                     LISCELELRS"
BASE COUNT          820 a         1536 c         1545 g          857 t
ORIGIN      
        1 agggccatcg cgtcgaagtc ttcgagaagt ctcccggcat catcgccaac ggcaccggca
       61 tcctgctgca accagccggg atagacgttc tgcacgggct cgatcttgcg gactccgcat
      121 tcgagcgcgg ttgcgcgatc gaacgtgtgg tggcgcgttc gcgcggcgct cgtcggatgg
      181 atctcaacta cgctgatctg cgtcctggcc tttgcgcatt gggcatccgg cggcccgcgc
      241 tcgcggcgct cctccacgat gccgcgcggt cggcgggtgc ggtgttccat ttcggtgtgg
      301 ctgtcgaaag gctgtcggag gcacccgatc acgtctcact actcgagcgc ggcggatcga
      361 ccgctcacgg gccgttcgac atcgcggtga tctgcaatgg tctcaattca cgattgcgtg
      421 accgcgtttc gcctggcgcg aaggtccggc cgcattcgcg cggtgtgttt tccgtggtgg
      481 cgccgatgcc ggcgggactg tccggcgagg tcctgctgca agccatgaac ggctggcggg
      541 atggcgtcgg tctgctgccg atcggacgcg gcgacggagc gacaccgctc gtgagtttct
      601 tctggaatgc gcgcgagagc gaccggccgc gtctcgaaac cgacggatat ccggcgtggt
      661 gcgattacat cgacggcttc tgccccgagg cgggagggct gctgcgaagt ctttcaggat
      721 tcgacgcact cacgtggtcg accacggccg atgtcgcatt gagccgctgg catggcgcac
      781 gcacgctggt gatgggcgat gcggcccttg cgttgaatcc acaactcgga ttgggcgcga
      841 cgagggcgtt gctggatgcg caggccctga gcgaagtgtt gcgcgatggc ggtgtcccgg
      901 cagcgctgcc ggcctatcaa gcccggcgca gacctcaggt cagccgctac gcgcgcgtaa
      961 gccgcttctg gtcgcggctc gatggcagcg gattctcacc gctgcgccgc cgactgttcc
     1021 tggccgccgc gaacggcttc ccggcgctgc gccgccggat gctgcaacac gtctgcggct
     1081 acgcgtagcg cgatcagcca aacttcgcgg gcgtcagcgt gagactgcga cctttcgcga
     1141 cgcggacctt ggcaaccttc tcccgatacc gaacctgcgc gactccgccc ggtgcgccgc
     1201 gaagctgcgc cgagaccagt tcgccatcgc gccaggccat gtccagctcg aagccgccgc
     1261 gtgcgcgcaa gccccgtacc gaccccgtgc tccaggcgcg cggcagcgcc ggcaatagct
     1321 cgatctcgcc gtccacacac tgcagcagca tttccatcat gccgttggcg ccgccgaagt
     1381 tgccgtcgat ctgaaacggc ggatgcgcat cgaacatgtt gggataggtg cgcgacggat
     1441 cgagcaatag tttgatgaca ccgtgcgtgt gctcggcatc gcgcaaccgc gcccagagat
     1501 tgatgcgcca cgcgatcgcc cagcccgtcg acagatcccc gcgcaactcg agcgagcgct
     1561 tcacggcagc ggccagttcc ggcgtgcgcc gcaccgtgat ctgttcaccc ggaaacagcg
     1621 catagagatg agacacgtgg cgatggtccc gttcgggcgc ttccatgtcc cagtcttcca
     1681 tccactcctg gagctggccg gccttgccga tgcgatccgg cggaagcttc gcgcgcgtgg
     1741 cggcgcaggc cgcgcggaac tcggcatcct tccccaggat ctccgcggcg tgaatcgtgt
     1801 tggcgaacag gtcgcgcagg atctggctgt ccatggctgg cccggcgcag atggccgcgc
     1861 cgtgcggatg gaggttctcg ggcgagatcg acgggctcgt gaccaggtat ttgcccgacg
     1921 ggtccttgac cagcgtatcg aggaagaatt gcgccgcgcc cctcagcagt ggatagacgt
     1981 cggcgagata ggcgcggtcg cgcccgtagt cgtagtgatc ccagaggtgt ttgcacagcc
     2041 aggcgccgcc cgtgggccac attccccact tcgcgccgtc gatgggaccc gaggcacgcc
     2101 acaggtcggt gttgtgatgc gcgacccagc cgcccgcacc gtagttgacg cgcgccgtaa
     2161 tcgcgcccgt caccgcgaga tccttgacca tcgccaccag cggctgcacg cactcggcga
     2221 ggttcgcagg ctccgcgggc cagtagttca tttcggtgtt gatgttgatc gtgtacttcg
     2281 agccccaggg cgccaacagc ttgtcgttcc acaagccctg gagattcgcg ggctgcgtcc
     2341 ctgggcgcga gcaaccgatc agaagatagc gcccatactg gaaatacagc gccgcaagtt
     2401 gcgggtcatc gctggtttgc gagttgcgga tgcgcaggtc ggtgggctgc tccgctgcgg
     2461 gcgtcgcggg tagttcgaac gacacgcggc caaacagttt gcggtggtcg gccacgtgcg
     2521 ccgcatgcag tgcatcgaaa ctcttgcccg ccgccgcctg gatctgcgcg cgcgtgatgg
     2581 cttcgggatt gccggacaca tcatcgaagc ggcggaagct cgtggcggcc gctaccagca
     2641 ccagcgcgga gtccgcgccg gtcacgacga actcgccgtc gcgcagttgc atctgcccgc
     2701 cctgcggaat caccagcgcc ctcgcctgaa agcgcagcgc ggcggcgatg ccctgctgct
     2761 cgacgttgcg gccgttgagc acgaggtgtg caccatcgat ggccacagcg gccggcatcg
     2821 gcgtctcgaa accggcctgg aagttcaacg cgccgggtcg atccgccgtg atgcgtgtga
     2881 cgatgacctg atcgaccgcc gtcgcaaacg actcgcgcgt gaacttcacg ccgtcgcgct
     2941 gaaagcggac gtgtgcgcac gcggattgca ggtcgaggtc acgccggtag tcctgcgcat
     3001 aggatgacgc gccgaaagtc aggatcagat cgccgaccgt ctggtatgac ggcatgcgga
     3061 tcggccgcgc catcatcttc tgctcggcga gatcttgcgc ctcggcatat ttgcctgcgg
     3121 cgatcagcgc acgcacctcc ggcaatgcga gcaatgcatc gggattcgac gggtcatatg
     3181 gcccgcccgc gtacagcgtg tcttcgttga tctgcaaacg ctcgcgcgcg ataccgccaa
     3241 agatcatggc gccgagccgg ccattgccga tgggcagtgc ttcggtccat tgttccgcgg
     3301 gtttctgata ccacagcgtg agtggcgacg cgcccgcctc cgccgcgcct gctactcgaa
     3361 atgcactcgt ggcgcacgca cccagcgcga tgccaccctt caatgtctgc cggcgcgtaa
     3421 tcatggtcgc gaaaggtact acggccccaa cgtcgctggg aatgtgaatg tcagcatggg
     3481 accacgccga ctatcagata tgtcccctgc cgctttgcga acggcgcgcg aatactccgc
     3541 ccctgaccga aggagagccc atgccgtacg tgaccaccga cgaccacacg cagctctact
     3601 acaaggagtg gggccgcggc cggcccgttg tgttgctcca tggctggccg ttgagcgcgg
     3661 actcctggga cgaccaagcc ttcgcgctgg ccaacgccgg ttatcgggtg atcgcctatg
     3721 accgtcgcgg atttggcagg tcctcacaac cctggaacgg ttacgactac gacacgctga
     3781 ccgacgatct gcgctccgtc ctcaagttct gccaggtgtc cgaggccgcg ctcgtcggct
     3841 tctccatggg cggcggcgaa gtcgcgcgct acatgagcaa gtacaacggc gccggcatcg
     3901 cgcaggctgg cctcatcgcg tccatcgttc cctacaaact caagaccgaa gacaacccgc
     3961 acggcaccga actcaaggcg ctcgaggaga ccacggccgc catcaaggaa gaccggccca
     4021 ggttcctggc ggagttcttc aagaagtttt acggcgtgga caaggctgaa aaaccggtga
     4081 gcgacgaagt gttgcagtgg tcggcgaatg tcgccatgca ggccggactc aaggccacgc
     4141 tggcttgcgc ggattcgttt ttcaagacgg atttccggcc cgatctcgcg tcgttcaaag
     4201 tgccgacgct catcattcat ggcaccgcgg atcagaacgt atcgatcgat gcatcggcgc
     4261 gaccggccgc gcaaggcatc cggaactcga agctcatcga gtacgaaggc tcaccgcatg
     4321 gactgctggc gacagacaag caacgcgtga tcgatcacct gctggcgttt ttgaaaggct
     4381 gacgccgctc agcttcgcag ttcgagctcg caggaaatga gctgcacgtt ggacaacgcc
     4441 ggatcgacag tgacgtgcaa ccagtaccgg ccgggctttt ccatcatcgt gaggaagatc
     4501 ggaacgcagc ggttcacgac gaaccggaag ctcacgttcg tctggtcgat gaaatagatg
     4561 gtcgatggcg tcgtcgcgtt gcgctggacg ccggaaagcg tcggctccgt gatgttcatc
     4621 ttgatcgacg tcagcgtgtc gaacgtgtag gtcgtgggcg ccgcgaccgc gagggatgac
     4681 gtgagcccca ggatgatggc ggcaaacgca agtttgatgt tcttcatgag agctccagat
     4741 cagggaattt cgtcgtca
//
LOCUS       DATDEU010000269         3958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1506944, whole genome shotgun sequence.
ACCESSION   DATDEU010000269 DATDEU010000000
VERSION     DATDEU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506944"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /gene="ilvN"
                     /locus_tag="VIV63_06565"
     CDS             complement(<1..441)
                     /gene="ilvN"
                     /locus_tag="VIV63_06565"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY6124295.1"
                     /translation="MRHIIAIHLQNEAGALTRVTGLFSTRGYNIESLSVAATDDPSVS
                     RVTLVTTGADSVILQIVNQLNKLIDVVAVEDLTRGEHIERELVVLKLRVEPQLVDSVR
                     GYVVRAGGRVLEPSPEGFVVELTASEAEVNQFVSDLGERTDIVEV"
     gene            541..2430
                     /gene="ilvB"
                     /locus_tag="VIV63_06570"
     CDS             541..2430
                     /gene="ilvB"
                     /locus_tag="VIV63_06570"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY6124296.1"
                     /translation="MAKPPTKSTNIRDLGASAKPAAPTIAHPLAGQKMTGAEMIVQVL
                     ADEGVSTIFGYSGGAILPTYDAVFCFNEDRAKKGEPQMPLIVPANEQGAGFMAAGYAR
                     ASGRVGVAIVTSGPGATNTVTPIRDSMADSIPIVVICGQVPTSAIGSDAFQEAPVPSI
                     MGSVAKHIFLVTDPDKLETTVRTAFEIARTGRPGPVVIDIPKDVQNWSGEFKGTGTLP
                     MPGYRKRLETLTTDIIDATEANHFFEMLGESQRPLIYAGGGVINGNASEELREFSETF
                     NIPVVTTLMGIGGVDTTSPLAMRMLGMHGQAFANYSVDDCDFLIAIGARFDDRVAGNP
                     AKFAPGAKYIAHFDIDRAEINKVKRVQWSHVGMLPEALRMLTSHGKRSGFTRDRSKWL
                     AYLADLKRTYAMNYDRASEKIQPYYVIEEINRVTKGEAIISTGVGQHQMWAAQYCDFR
                     HPRLWLTSGSMGTMGFGLPAAIGAQFAQRDKLVIDIDGDASVRMNFGELETVTTYDLP
                     VKVVILNNNGDGMVKQWQKLFFKGRLSASEKTLHTKDFIKAAQADGFKYAVRLDKKAD
                     VPRVIEEFLAFKGAAFLEVIIDADAGVYPMVGPGQPYEAMITGDWIPSRKKVDVKPPG
                     ASEMF"
     gene            2445..2864
                     /locus_tag="VIV63_06575"
     CDS             2445..2864
                     /locus_tag="VIV63_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6124297.1"
                     /translation="MKYLHTMVRVTDVDASLKFYCDALGLEVLKRNDYPNGKFTLIFL
                     AAPGDTTAQVELTHNWEPEAYGGGRNFGHLAYAVDDIYATCERLQKHGVTINRPPRDG
                     RMAFVRSPDNISIELLQKDGSLPPREPWVSMANTGVW"
     gene            2858..3589
                     /locus_tag="VIV63_06580"
     CDS             2858..3589
                     /locus_tag="VIV63_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY6124298.1"
                     /translation="MVSTEVPERRAAFRAGQRAILPSLPGAFAWGMVSGVAMVKGGLS
                     VPWAILMSLTVYAGSAQLAALPLIAAGAPLWVIVATGLITNLRFVIYSAALRPHFADE
                     PLRKRAALGFFMTDFTFTRFMRAVQEGELPRHRDSWFAGVCANNWITWQVSAMTGIIG
                     ASYIPTDWGLEFTGTLALVALVGPSLNARPALVGAVVAAVVALIAHPMPFKLGLFCGA
                     IAGIVAATISDALQQRSPPQESERA"
     gene            3586..3927
                     /locus_tag="VIV63_06585"
     CDS             3586..3927
                     /locus_tag="VIV63_06585"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY6124299.1"
                     /translation="MIEPVAGIALALVIIGLALSTFVTRTSFLIAGAKLRLGPRVEVA
                     LRYAPVCTLAAIIVPDVLIHPPGHAIDWSLLNPRLIGTLAAALWLCWSRNIIGCLVLG
                     MGAYSLARLYL"
BASE COUNT          695 a         1325 c         1242 g          696 t
ORIGIN      
        1 gacttcgacg atgtcggtgc gttcgcccag gtcgctcacg aactggttga cctcggcttc
       61 actggccgtg agttcgacca cgaagccttc gggcgagggc tcgagtaccc ggccgccggc
      121 cctgaccacg taaccccgca ccgaatcgac gagctgcggt tcgacccgta gtttgagcac
      181 caccaattcg cgctcgatgt gttcgccgcg cgtcaggtct tcgaccgcca ccacgtcgat
      241 gagcttgttg agctggttga cgatctggag gatcacgctg tccgcgcccg tggtcaccag
      301 cgtgacgcgc gataccgagg ggtcgtccgt cgccgctacc gacagcgact cgatgttgta
      361 gccgcgggtg gaaaacaggc ccgtgacgcg ggtgagcgcg ccggcctcgt tctgcaggtg
      421 gatggcgatg atatgacgca tggttctggg catctggcgg ggcggaaacg gcggccagaa
      481 tagcggtttg cggccaatcc attcaatcaa acaatacaaa atgctaggct cccgcgggcc
      541 atggcaaaac ctcccacgaa atcgacaaat atccgtgact taggcgcatc cgcgaagccg
      601 gccgcgccca cgatcgccca tccgcttgcg ggtcagaaga tgaccggcgc cgagatgatc
      661 gtgcaggtac tcgcggacga gggtgtatcc accattttcg gttacagcgg cggcgccatc
      721 ctgcctacct acgacgccgt cttctgcttc aacgaagacc gcgccaagaa gggcgagccg
      781 cagatgccgc tcatcgtgcc ggccaacgag cagggcgcgg gattcatggc cgcgggttac
      841 gcgcgcgcca gcggccgcgt gggcgtggcc atcgtcactt caggtcccgg cgccaccaac
      901 accgtgacgc cgatccgcga ttccatggcg gactccatcc cgatcgttgt catctgcggc
      961 caggtgccca ccagcgccat cggctcggac gcgttccagg aagcgccggt gccttccatc
     1021 atgggttcgg tcgccaaaca catcttcctg gtcaccgacc ctgacaagct cgagaccacg
     1081 gttcgcaccg cgttcgagat cgcgcgcacc ggccgtccgg gcccggtggt catcgacatc
     1141 ccgaaagacg tgcagaactg gtcgggcgaa ttcaagggca cgggcacgtt gcccatgccg
     1201 ggctaccgca agcgtctcga gacgctgacg accgacatca tcgacgccac cgaggctaac
     1261 cacttcttcg agatgctcgg cgagtcgcag cgtccgctca tctacgcagg cggcggcgtc
     1321 atcaacggca atgcgtcgga agaactgcgc gaattcagcg aaaccttcaa cattcccgtg
     1381 gtcaccacgc tcatgggaat cggcggcgtc gacaccacga gcccgctcgc catgcgcatg
     1441 ctgggcatgc acggtcaggc tttcgcgaac tactcggtcg acgattgcga cttcctcatc
     1501 gccatcggcg cacgcttcga cgatcgcgtc gcgggcaatc cggcgaaatt cgcgccgggc
     1561 gccaagtaca tcgcgcactt cgacatcgac cgcgcggaga tcaacaaggt gaagcgcgtg
     1621 cagtggagcc acgtgggcat gttgcccgag gcgctgcgca tgctgacttc gcatggcaag
     1681 cgttccggct tcacgcgtga ccgttcgaag tggctcgcct acctcgcgga cctcaagcgc
     1741 acgtacgcca tgaactacga ccgcgccagc gagaaaatcc agccttacta cgtcatcgaa
     1801 gagatcaacc gcgtcacgaa aggcgaggcc atcatctcca caggcgtcgg ccagcaccag
     1861 atgtgggccg cgcagtactg cgatttccgc catccgcgtt tgtggctcac ctcgggttcc
     1921 atggggacca tgggctttgg cttgcccgcc gccatcggcg cgcagttcgc gcagcgcgac
     1981 aagctggtca tcgacatcga tggcgacgcc tcggtgcgca tgaacttcgg cgagctcgag
     2041 accgtgacga cgtacgacct gcccgtgaag gtcgtcatcc tcaacaacaa cggcgatggc
     2101 atggtgaagc agtggcagaa gctgttcttc aagggccgcc tgtcggcgtc cgagaagacg
     2161 ctgcacacca aggacttcat caaggccgcg caggccgacg gcttcaagta cgccgtgcgg
     2221 ctcgacaaga aggccgacgt gcctcgcgtc atcgaagaat tcctggcgtt caagggcgcc
     2281 gcgttcctcg aggtcatcat cgacgcggat gccggcgtgt atcccatggt gggcccgggc
     2341 cagccgtacg aagccatgat caccggcgac tggattccgt cgcgcaagaa ggtcgacgtg
     2401 aagccgcccg gcgccagcga gatgttctga cccgctgagc gacgatgaaa tacctgcaca
     2461 ccatggtgcg agtcaccgat gtcgatgcct cgctgaagtt ctactgcgac gcgctcggcc
     2521 tggaagtcct caagcgcaac gactatccga acggaaagtt cacgctgatc ttcctcgcgg
     2581 cgcctggcga caccaccgcg caggtcgagc tcacgcacaa ctgggagccg gaggcttacg
     2641 gtggcggccg caacttcggc cacctggctt acgccgtcga tgacatctac gcgacgtgtg
     2701 aacgactgca aaaacacggc gtgaccatca accgtccgcc gcgcgacggc cgcatggctt
     2761 tcgtgcgttc gcccgacaac atctccatcg aactactgca gaaagacggc tcgctgccac
     2821 cgcgcgagcc ctgggtgtcg atggccaaca ccggcgtgtg gtaagcacgg aagtccctga
     2881 acgccgcgcg gcgtttcgag cgggccagcg cgccatcctc ccttcgttgc ccggcgcatt
     2941 cgcgtgggga atggtcagcg gcgtcgcgat ggtcaaaggt ggcctgagcg ttccctgggc
     3001 catcctgatg tcgctcacgg tgtacgcggg ttcggcgcag ctcgcggcgc tgccgctcat
     3061 cgccgccggt gcgccgctgt gggtcatcgt cgccaccggg ctcatcacca accttcgctt
     3121 cgtcatctac agcgccgcgc tgcggccgca cttcgccgac gagcccctgc gcaaacgcgc
     3181 cgcgctcggc ttcttcatga cggactttac gttcacgcga ttcatgcgcg cggtccagga
     3241 aggggagctg ccccggcacc gcgactcgtg gttcgcgggc gtctgcgcca acaactggat
     3301 cacgtggcag gtgtcggcga tgacgggaat catcggcgcc agctatatcc ccacggactg
     3361 gggactcgag ttcaccggca cgctggcgct cgtggcgctc gtcgggccca gtctcaacgc
     3421 gcggcccgca ctcgtcggcg ccgttgtcgc ggccgtggtg gctttgatcg cgcatcccat
     3481 gccgttcaag ctcgggttgt tctgcggcgc catcgcgggc atcgtcgccg cgacgatttc
     3541 cgatgcgctc cagcagcgct cgccgccaca ggagtcggag cgcgcatgat cgaacccgtt
     3601 gcgggaatcg cgctggcgct ggtgatcatc ggcctcgctc tatctacgtt cgtgacgcga
     3661 acctcgttcc tgatcgccgg ggcgaagctg cgcctggggc cgcgcgtcga ggtggcgctg
     3721 cgctacgcgc cggtgtgcac gctggcggcc atcatcgtgc cggacgtgct catccatccg
     3781 ccggggcatg cgatcgactg gtcgctgctc aatccgcggc tcatcggaac gctggcggcc
     3841 gcgctgtggc tctgctggtc gcgcaatatc atcggctgcc tcgtgctcgg catgggcgcg
     3901 tattcactgg ccagactata tctatgattg cgcggcttgc cgtagatagt cggccggc
//
LOCUS       DATDEU010000270         5408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_193682, whole genome shotgun sequence.
ACCESSION   DATDEU010000270 DATDEU010000000
VERSION     DATDEU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193682"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VIV63_06590"
     CDS             complement(<1..346)
                     /locus_tag="VIV63_06590"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY6124300.1"
                     /translation="MARETSTAGLQLLLAMAIFSTAPSRADEDCPPTAAERAEADIKP
                     KPPAPDVPAEPPDERVTIEADDDDFKIDADGNAVVSGNVVLRQGDKVIRGDRMEYNAQ
                     SGQAKIDGAVEFT"
     gene            368..1666
                     /locus_tag="VIV63_06595"
     CDS             368..1666
                     /locus_tag="VIV63_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY6124301.1"
                     /translation="MLKNDARLSHIRAWLTRDLGWRIGRISVASADASFRRYFRVSRG
                     DVDPAAWAPKADTLIIMDAPPGKEDIAPYLKVTSLLEQAGAHVPHVHAADAKRGFIVM
                     EDLGDTQYLSLLKTGRGVDKLYGDALTTLANIQVRGLPASMALEPYDRAPIERELNLM
                     PEWFLAKHLRLELSPEERALLTVTNEFLINEALLQPQVFVHRDFHSRNLMVLPKGGPG
                     VIDFQDALRGPIGYDLVSLLKDCYISWSRERVERWVKGYRRVLGNLGANVGDSEYQFM
                     RWFDLIGAQRHIKVLGVFARLWHRDGKIGYLADLPLVLDYVIDACRRYPELVEFGRWL
                     EWRVLPELPLANDRQKEKPVRRRAKPKAKTKAKTKTRPKPKRRKPVKAKAKVKVRKKT
                     KVKAKSARVVRKSSKTRKARKKVRSRKLVVRRRRTRRSHK"
     gene            1672..2337
                     /locus_tag="VIV63_06600"
     CDS             1672..2337
                     /locus_tag="VIV63_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY6124302.1"
                     /translation="MVLAAGRGERMRPLTDREPKPLLRAGGKRLIEYHLERLAAGGFR
                     EVIVNTAWLGDMIEAALGTGKQFGLSITYSHERPEALETGGGIFRALPLLGSAPFLLV
                     NGDVWTDIDLGALRRPPPEGSLAHLVLVPNPPQHARGDFLLENGLVAEGEGLRRTYSG
                     VGIFRPELFAGCEPGKFPLLPLLRKAIAQRALSGELHEGRWYDIGTTERLEALDAQLS
                     RVS"
     gene            2347..3795
                     /locus_tag="VIV63_06605"
     CDS             2347..3795
                     /locus_tag="VIV63_06605"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEY6124303.1"
                     /translation="MKKLSIAVVILVVVLLLVTPWGVGGFAEKRMDTGLEQLVEAAPY
                     LTVVERKYTKGWFKSEHVVTFEAFGGWMKALSPKAVEDDAADDAGTVEVPEEPAKPEE
                     PLRFTVRNEVLHGPVLGLTGFGLARVNSHLVFDDKVRKELEEVFGPKAPLEVTTRLGF
                     FGGGRTTFKSEGRTIKPKDSQTEITWETFKLAVGYSGNLDEYDVDGTWPKLEMSDKSA
                     RTQFVMTNMTMDGDGKRVRGDLYDGDFDLSVEKVTFFDDNQNEVEVSQAQYLVTSESK
                     GEFTGMSAKLGTGEIKSKVLSAQGVEIKEAHYDLSVRRLHTETLEKVITAFKATYAKP
                     LATTVEGDEVMFAPLKAHGMELLKYDPEFVIDRIGVATPEGDGYVMGVVKLKGATAED
                     FGVGSMALIGKIDADITFNVSEKMIQKFPNGSTAAGAAVDSGYAKREGERLICKIVFK
                     NGELTVNGKPQAIPGLGGPPPGVESMEPPPQE"
     gene            3811..4734
                     /gene="lipA"
                     /locus_tag="VIV63_06610"
                     /pseudo
     CDS             3811..4734
                     /gene="lipA"
                     /locus_tag="VIV63_06610"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            4930..>5408
                     /gene="lipB"
                     /locus_tag="VIV63_06615"
     CDS             4930..>5408
                     /gene="lipB"
                     /locus_tag="VIV63_06615"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013357092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY6124304.1"
                     /translation="MQRITDARDENTPDEIWLLEHPRVFTLGLNADPAHVLGAGDIPV
                     VKIDRGGQVTYHGPGQLVVYPLIDIRRAGMGVRDLVTALERAVIAYCASLGITAECRK
                     NAPGVYVAGKKIASVGLRIRRGASYHGLDFNVNMDLEPFQRINPCGYAGLQMTQLAEL
                     "
BASE COUNT         1062 a         1693 c         1756 g          897 t
ORIGIN      
        1 aggtgaactc caccgcgccg tcgatcttgg cctggccgct ctgcgcgttg tattccatgc
       61 gatcgccgcg aatgaccttg tcgccctggc gcaggacgac attgcccgac accaccgcat
      121 tgccgtccgc gtcgatcttg aaatcgtcgt cgtcggcttc gatggtgacg cgttcgtcgg
      181 gtggttcggc gggaacgtcg ggagccgggg gtttgggttt gatgtcggcc tcggcccgtt
      241 cggcggcggt cggcgggcag tcctcgtcgg cccgggacgg cgccgtcgaa aaaatggcca
      301 tcgccaatag caattgcagt cccgcagtcg aagtctctct cgccatttgc gcactatact
      361 tatacgaatg ctcaagaacg acgcgcgact ctcacacata cgtgcttggc tgacacgcga
      421 cctgggttgg cgcataggcc ggatcagcgt cgcgtcggct gacgccagct tccgccgcta
      481 cttccgtgtc tcccggggcg acgtcgaccc ggcggcgtgg gcgccgaaag ccgacacgct
      541 catcatcatg gacgcgccgc ctggcaagga agacatcgcg ccctatctca aggtcacctc
      601 gctgctggag caggccggag cgcacgtgcc ccacgtccac gccgcggacg cgaagcgtgg
      661 cttcatcgtc atggaagatc tgggcgacac ccagtatctc tcgctactca agacgggccg
      721 cggcgtcgac aaactctatg gcgacgcgct cacgacgctg gccaacatcc aggtgagggg
      781 tcttcccgcc tcgatggcgc tcgagcccta tgaccgtgcg ccgatcgagc gcgaactcaa
      841 cctcatgccg gaatggttcc tcgccaaaca tctgcggctc gaactctcgc ccgaagagcg
      901 tgcgctgttg acggtcacca acgagttcct gatcaacgag gcgctgctgc agccgcaggt
      961 gttcgttcac cgcgacttcc actcgcgcaa cctcatggtc ctgcccaaag gcggtccggg
     1021 cgtcatcgat ttccaggacg cgctgcgagg gcccatcggc tacgacctcg tctcgctgct
     1081 caaggactgc tacatcagct ggtcgcgtga gcgggtcgaa cgctgggtga agggttatcg
     1141 ccgcgtgctc ggcaacctgg gcgcgaacgt cggcgacagc gaataccagt tcatgcgctg
     1201 gttcgatctc atcggcgcgc agcggcacat caaggtgctc ggcgtcttcg cgcggctctg
     1261 gcatcgcgac gggaagatag gctacctcgc cgacctgccg ctggtgctcg actacgtgat
     1321 cgacgcgtgc cgccgctatc ccgagctcgt cgagttcggg cgatggctcg agtggcgcgt
     1381 gttgccggag ctgccgctcg cgaatgaccg gcagaaagag aagccggtgc ggcgcagggc
     1441 gaagcccaag gcgaagacga aagcgaaaac caagaccagg ccgaagccga agcgccgcaa
     1501 gccggtcaaa gccaaagcca aagtcaaagt caggaagaag acaaaggtaa aggcgaagtc
     1561 cgcgcgagtc gttcgcaagt cgagcaagac ccgcaaggcg cgcaagaagg tccgcagccg
     1621 aaagctcgtc gtccggcggc gtcggacgcg acgttcacac aagtgaaggc catggtgttg
     1681 gccgcgggac gcggcgaacg catgcgtccg ctgaccgacc gcgagcccaa acctctgttg
     1741 cgcgccggcg gcaaacgact catcgaatat catctcgagc ggctggccgc cggcggtttc
     1801 cgcgaagtga tcgtcaacac cgcgtggtta ggcgacatga tcgaggccgc gctcggcacc
     1861 ggaaagcaat tcgggctttc cataacctat agccacgagc ggccagaggc cctggaaacg
     1921 ggcggaggca tttttcgcgc gctgccgctg ttaggatccg caccgttcct gctggtgaac
     1981 ggcgatgtct ggaccgacat cgatctaggc gcgctgcgcc gtccaccgcc cgagggttcg
     2041 cttgcgcacc tggtgctggt gccgaacccg ccgcaacacg cccgcggcga tttcctgctc
     2101 gagaacggac tcgtggcgga aggcgaggga ctgcggcgca cgtattccgg tgtcggcatt
     2161 tttcgacccg aattgttcgc gggctgcgag ccaggaaaat ttccgctgtt gccattgctg
     2221 cgcaaggcga tcgcccagcg cgcgctcagc ggtgaattgc atgaaggccg gtggtacgac
     2281 atcggcacga cggagcggct cgaggcgctc gacgcgcagc tgtcgcgagt ttcttaggga
     2341 ggcagagtga aaaagttaag tattgcggtc gtgatcctcg tggtggtgct cctgttggtg
     2401 acgccgtggg gcgtgggcgg attcgctgag aagcggatgg acacgggact cgagcagctg
     2461 gtcgaagccg cgccgtatct caccgtcgtc gaacgcaagt acacgaaggg ttggttcaag
     2521 tccgaacatg tagtcacgtt cgaggcattc ggtggctgga tgaaggcact gagccccaag
     2581 gcagtcgaag acgatgccgc cgatgacgcg ggaacggtcg aggttcccga agagccggcc
     2641 aaacccgagg agcctctgcg gttcaccgtg cgtaatgaag tgctgcatgg cccggtgctc
     2701 ggactcacag gcttcggttt ggcgcgcgtg aattcgcacc tggtgttcga cgacaaggtc
     2761 cgcaaggaac tcgaagaagt cttcggcccg aaagccccgc tcgaagtcac cacgcgtctc
     2821 ggtttcttcg gcggcggaag gaccaccttc aagtccgaag gccgcacgat caagccaaag
     2881 gattcgcaga ctgaaatcac ctgggagacc ttcaagcttg ccgtgggcta ctcgggcaac
     2941 ctcgacgagt acgacgtcga tggcacgtgg ccgaaactcg agatgagcga caagagcgcc
     3001 aggacgcagt tcgtcatgac gaacatgacg atggacggcg atggcaaacg cgtccgcggc
     3061 gatctgtacg acggcgactt cgatctgtct gtcgagaagg tcaccttctt cgacgacaac
     3121 cagaacgaag tcgaggtcag tcaagcgcag tacctggtga ccagcgaaag caaaggcgag
     3181 ttcacgggca tgagcgccaa gctgggcacg ggcgaaatca agtcgaaagt cctctcggcg
     3241 cagggcgtcg agatcaagga ggcgcactac gacctgtcgg tgcgccggct gcataccgag
     3301 acgctcgaga aagtcatcac ggccttcaag gcgacgtatg cgaagccgct cgcgaccacg
     3361 gttgaaggcg acgaagtcat gttcgcgccc ctcaaggcgc acggcatgga gttgctcaag
     3421 tacgacccgg agttcgtcat cgatcgcatc ggggtcgcga cacctgaagg cgatgggtac
     3481 gtcatgggtg tggtcaaact caagggcgcc acggccgaag actttggcgt cggcagcatg
     3541 gccctcattg gaaaaatcga cgccgacatc acattcaatg tgtcggagaa gatgatccag
     3601 aaattcccca acggatcgac cgccgcgggt gcggccgtcg attcgggcta tgccaaacgc
     3661 gagggcgaac ggctcatctg caagatcgtc ttcaagaacg gcgagctgac cgtgaacggt
     3721 aaacctcagg caattccggg acttggcggc ccaccgcccg gcgtggaatc gatggagccg
     3781 cctccgcagg agtagaattc ggaccccacc atggcgtcac tcaaggacat tcccgtcgtc
     3841 accgaatcgc gcgcgattcg cagtggcgag aagtacgtta ccccgcaggg ctttaccgcc
     3901 atcaaggacg gtcagaagca gcgtggcgcc agtgctccgg ccaccggccg caaaccggcc
     3961 tggatccggg cgcagcttcc ggtgggcgcc gggttcggcg cggtgaaggg gatcgtgtcc
     4021 gagcaccggc tcgcgaccgt ctgcgaagaa gccaagtgtc cgaacatcgg tgaatgctgg
     4081 aacgccggca cggccaccat catgttgatg ggcgccgtgt gtacgcgggc ctgccgtttt
     4141 tgcagcgtgg acaccggcaa cccgcgcggc tggctggatg cggaagagcc cgagaacacc
     4201 gcgcgcagcg tcgagctcat gaagctcaag tacatcgtgc tcacctcggt gaatcgcgac
     4261 gatctgcccg acggaggcgc ggagcactac gccgcggcca ttcgcgcgat caagcggcgc
     4321 acaccacacg tggccgtcga agcgctgacg ccggatttcc agggcgtgat gcgcgacgtc
     4381 gagacggtcg tggattcggg cctggaggtg ttcgcgcaga acgtcgagac cgtgaagcgc
     4441 ctgacgcacc cggtgcgcga tgcgcgtgcg agctacgagc agacgctcgc ggtgctcgaa
     4501 catgcgaaga agcatcgtcc ggaagtgctg accaagacca gcctcatgct cgggctcggc
     4561 gagaccgacg aagagattgc cgcgaccatg gacgacttgc gcgccgtgaa cgtggacctg
     4621 ctgacgctgg ggcaatacct gcgtcccacc gtgcatcacc tcgaagtgca gcgtttcgtg
     4681 acgcccgccg aattcgacca gtaccgcgag tgggcgctgg ccaaaggttt ccgcgagtgc
     4741 gtcgctggcc ccctcgtgcg ctccagctac cgcgccgaac aggcgctggc cggcaacaac
     4801 gccgggttga agaatgtaaa aaatggggac actccccatt tgctggcaaa aggggccgca
     4861 cctgccgata gcggcgtgcc tgccgtcaag tttctcggac gcgtggacta cgaacccacc
     4921 tggcgcgaga tgcagcgcat cacggacgcg cgcgacgaaa acacgccgga tgaaatctgg
     4981 ctgctcgagc acccgcgggt gttcacgctc gggctcaatg ccgaccccgc gcacgtgctc
     5041 ggcgccggtg acatcccggt ggtcaagatc gatcgcggcg gccaggtcac ctatcacggt
     5101 cccggccagc tcgtcgtcta tccactcatc gacatccgcc gcgccggcat gggcgtgcgc
     5161 gacctcgtga ccgcgctcga acgcgccgtc atcgcctact gcgcgagcct cggcatcacc
     5221 gccgagtgcc gcaagaacgc gccgggcgtc tacgtggccg gcaagaaaat cgccagcgtc
     5281 ggcttgcgca tccgccgcgg cgcgagttat cacggcctcg atttcaacgt gaacatggat
     5341 ctcgagccgt tccagcgcat caatccctgc ggatatgcgg gcttgcagat gacgcagctc
     5401 gcggagct
//
LOCUS       DATDEU010000271         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1652917, whole genome shotgun sequence.
ACCESSION   DATDEU010000271 DATDEU010000000
VERSION     DATDEU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652917"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VIV63_06620"
     CDS             <1..289
                     /locus_tag="VIV63_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEY6124305.1"
                     /translation="EMLRNVIARGDGMQNDAEFAEEVRSVVLPPGSWCIGKCVDEVRK
                     AGADVAFTGIRRHGILGREPTGDTEFQDGDIVVIYGTPEALEHAEAVLLAG"
     gene            307..627
                     /locus_tag="VIV63_06625"
     CDS             307..627
                     /locus_tag="VIV63_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124306.1"
                     /translation="MPGWKVVAWMSPFLAVLFPLLHGWSVGFKPEAMVLLAAAGAALG
                     VIAAPTIEPASFAYPVAWQVFFSVLACIFVAIQQQAGVPGFAFALVGGVVIGLTAKWW
                     TQGL"
     gene            complement(635..1567)
                     /locus_tag="VIV63_06630"
     CDS             complement(635..1567)
                     /locus_tag="VIV63_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6124307.1"
                     /translation="MNQEKGRQRLGMLFVAASAILFASKGLFGKALYQRGVGFELLVT
                     VRALLAMPLFAWVAWRANTPARSGHERRAMPWRAVAASVIAGVTCYYVGAFLDFWALT
                     LIDASIERVLLFSYPAMVVLLGSFLTRRLPERRVVVAMLITYAGIWFVMGGIDLRELQ
                     QNLFGAGLVLIAALTTAIYFLIGERYTHELGSTRFAAIGMGAAAVVLALHFAMFRSFD
                     ELRTLQGYDWFLLAVLGVACMFVPGLLQAEGMRRVGAQRAAIASTIGPPTTIFLAALF
                     LNERLNGWQLLGSAMIVGSVLVLSWPKRVVADEP"
     gene            1637..>2381
                     /locus_tag="VIV63_06635"
     CDS             1637..>2381
                     /locus_tag="VIV63_06635"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY6124308.1"
                     /translation="MKRDDIQFPARHSALRDDVHVLGALIGDVLKEQGGDTLFDLVEK
                     DRRLSIRRRAGDKEAAAELTVQLRGRAPQVARDLARAFSSWFRAVNLAEKVHRIRRRR
                     GYFLEASERAQPGGVEAALIELKNRGFSLAQVLELLKKVSVEPVFTAHPTESARRTML
                     RKNQRIAELLLDRLDPTLTPQELRQNWSRVRTEVTTAWQTDDHPRERLPVADEREHVV
                     FYLAEKVHRIRRRRGYFLEASERAQPGGVE"
BASE COUNT          411 a          828 c          784 g          358 t
ORIGIN      
        1 cgagatgctg cgcaacgtca tcgcgcgtgg cgatggcatg cagaacgacg cggagttcgc
       61 cgaagaggtg cgctcggtgg tgttgccgcc cggttcgtgg tgcatcggca aatgcgtgga
      121 cgaagtacgc aaggccggcg ccgatgtcgc gttcaccggc atccgccgcc atggcattct
      181 cggccgcgag cccacgggtg ataccgagtt tcaggacggc gacatcgtgg tgatctacgg
      241 cacacccgag gcgctcgaac acgccgaagc ggtgttgctg gcgggttgat gccgcgtcaa
      301 cgccccatgc caggctggaa agtggtggcg tggatgtcac ctttcctggc cgtgttgttt
      361 cccctgctgc atggctggtc cgtgggcttc aaacccgaag cgatggtgct gctcgccgcc
      421 gcgggcgccg cgctcggcgt cattgccgcg ccgaccatcg aacccgcgtc cttcgcctat
      481 cccgttgcct ggcaggtgtt cttctcggtg ctcgcctgca tattcgttgc catccagcag
      541 caggcgggcg tgcctggctt cgccttcgcg ctcgtcggcg gtgtcgtcat cgggctgacc
      601 gcgaagtggt ggacccaggg cctgtgacgc ttcgctaagg ctcgtcagcg accacgcgct
      661 tcggccagct cagcaccagc acgctgccga cgatcatcgc gctgcctaac aactgccagc
      721 cgttcaaccg ctcgttgaga aacagcgccg ccaggaagat ggtggtgggc gggccgatgg
      781 tgctggcgat ggcggcgcgc tgcgcgccca cgcgccgcat gccttcggcc tgaagcagcc
      841 cggggacgaa catgcaggcc actccgagca cggccagcag gaaccagtcg tagccctgca
      901 gcgtgcgcag ctcgtcgaac gagcggaaca tcgcgaagtg caaggccagc accacggccg
      961 cggcgcccat gccgatggcg gcgaaacggg tgctgccgag ttcgtgcgtg taacgctcgc
     1021 cgatcaggaa gtagatagcc gtggtcagcg ccgcgatgag cacgaggccg gcgccgaaca
     1081 ggttctgctg gagctcgcgc aggtcgatgc cgcccatgac gaaccagatg ccggcgtacg
     1141 tgatgagcat ggcgaccacg acgcggcgct ccggcagccg ccgcgtcagg aatgagccga
     1201 gaagcacgac catggcggga tagctgaaca gcagcacgcg ctcgatggac gcatcgatga
     1261 gcgtcagcgc ccagaaatcc aggaacgcgc cgacgtagta gcaggtgacg ccggcgatga
     1321 ccgaggcggc cacggcgcgc cagggcatgg cgcggcgctc gtgcccactc cgggcgggcg
     1381 tattggcacg ccacgcaacc catgcgaaca gcggcatggc gagcagggcc cgcacggtca
     1441 ccagcagctc gaaacccacg ccccgctggt acagggcctt gccaaacagc cccttgctgg
     1501 cgaacaagat ggccgaagcg gcgacgaaca gcatgcccag cctctgccgg cccttttcct
     1561 ggttcatgac tccatgattc cccagcgcgc cgcactgcgc catccctttc gcattcactg
     1621 tagaatcccg cgcttcgtga agcgcgacga catccagttc ccggccagac actcagcgtt
     1681 gcgcgacgac gtgcacgtgc tcggcgcgtt gatcggcgac gtgctcaagg agcagggcgg
     1741 cgacacgctg ttcgacctcg tcgaaaagga ccgccgtcta tctatccgcc gccgcgccgg
     1801 cgacaaggaa gccgcggccg agctcaccgt ccagcttcgc ggccgtgcgc cgcaggtcgc
     1861 acgcgatctc gcgcgagcct tctcgagctg gttccgcgcc gtcaatctcg ccgagaaagt
     1921 gcaccgcatc cgccgccgcc gcggctactt cctcgaggcc agcgaacgcg cgcagcccgg
     1981 tggcgtggaa gctgcgctca tcgagctcaa gaaccgcggc ttctcgctcg cccaggtgct
     2041 cgagttgctg aagaaggtga gcgtcgaacc ggtgttcacc gcgcatccca ccgagagcgc
     2101 ccgccgcacc atgctgcgca agaaccagcg catcgccgag ctgctgctcg accgcctgga
     2161 ccccacgctg accccgcagg agctgcgcca gaactggagc cgcgtgcgca ccgaggtcac
     2221 caccgcgtgg cagaccgacg accatccgcg tgaacgcctc ccggtcgccg acgagcgcga
     2281 gcacgtcgtg ttctatctcg ccgagaaagt gcaccgcatc cgccgccgcc gcggctactt
     2341 cctcgaggcc agcgaacgcg cgcagcccgg tggcgtggaa g
//
LOCUS       DATDEU010000272         4544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1070925, whole genome shotgun sequence.
ACCESSION   DATDEU010000272 DATDEU010000000
VERSION     DATDEU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070925"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="mreC"
                     /locus_tag="VIV63_06640"
     CDS             <1..870
                     /gene="mreC"
                     /locus_tag="VIV63_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY6124309.1"
                     /translation="FRFFCYALLSVVLMFYDRRGQELGRGWLDTARYGLQAAIYPLQL
                     AVNSPSAAWRWLEESFTTRETLRAEVDRLREDLRNQQLVTLRQSALERENATLRGLNA
                     LLPNVIDKRLVGEVINVEVSTLRQRLLVNRGGTHGVYKAQPVVTGDGVLGQVFRTGPF
                     SSEIILITDAEHALPVQVVRSGVRTIALGTGRSTSLELPYVPQNYDVKVGDLLVTSGL
                     GRVFPFGLPVARVTKVERDPTQPLAQIHAVPLAGIESDREVLFIWERTDHPASTATPD
                     ALAVEAAPPVKKP"
     gene            867..1346
                     /gene="mreD"
                     /locus_tag="VIV63_06645"
     CDS             867..1346
                     /gene="mreD"
                     /locus_tag="VIV63_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY6124310.1"
                     /translation="MNLARREPRWAVTFTVLLALIAQVLPLPDWLSVVRPSFVALVII
                     YWSIYAPHAGGIFAPWLAGLALDVFKGEVLAQNALAIALVAYIAMSLHQRLRNQTLVQ
                     QSLFVFAVLTLNEFVVWGIEGWSGHSVSTGWRWIQPMVGAMLWPFIALLVGRTHTRT"
     gene            1348..3318
                     /gene="mrdA"
                     /locus_tag="VIV63_06650"
     CDS             1348..3318
                     /gene="mrdA"
                     /locus_tag="VIV63_06650"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY6124311.1"
                     /translation="MGTVRIKDHWAEQRLFESRVLAAAFIIAGLTIILIGRLVVLQVM
                     RYDYYAELSQGNRVRVEPLPAQRGLILDRKGLVLAENRPAFQLELVRERVPDVEATLK
                     RLIEIGVLPADDLDETRRLVRSRRSFESVPIRLRLTDEEIARFAVHRFEFPGVDIATR
                     LTRFYPHGEHAVHALGYVAAISENDVKRLEKEKELSNYAGTTLIGKLGIEASYEKQMH
                     GVDGSRQILVNAAGRSVQRQGSMTPDLQEIKPVAGRDVVTSIDLPTQLVAEQGLIGRR
                     GSVVAIDPSTGDVIALVSTPGFDPNAFGRGLTKAEYALLRDNIDIPLLNRAIRGEYPP
                     GSTVKPVIALAGLANGVTTPQRTRACGGYFTLPGSRHRFRDWRPAGHGIVSMEAAIAQ
                     SCDVYFYELANMLGVDRLSEFLSHFGLGEVTGIDISGERKGILPSPAWKKAYFKTAAN
                     QVWFPGETVIFGIGQGYLIVTPLQLAHMTSIVASRGKSYRPRLVTAYREQSTGKIEPI
                     PPKLLETIDVATPENWQIAVNGMIRVMSGEGGTGRRSQVGAEYQIAGKTGTAQVFTVK
                     QNENYKAMEARLNERLLDHGWFVAFAPADAPKLAVAVLIENGKHGTAAAPIARRVLDQ
                     YLLGRTTTPEIPPHVVAPTGEVVVAPDAPGDE"
     gene            3324..4463
                     /gene="rodA"
                     /locus_tag="VIV63_06655"
     CDS             3324..4463
                     /gene="rodA"
                     /locus_tag="VIV63_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY6124312.1"
                     /translation="MYEPIEVGSRSRRTFTAAGRILGALKLDGPLLVGLGLITLYGLV
                     VLYSASGQNWNRVIDGTIRAALGAVAMCALAQVKPAFLRRLALLMWIVGVVLLIVVDI
                     TGHIGKGAQRWLDLGFIRFQPSEIMKLAVPMTCAWWLHDRPLPPDWKSLLALAVIILV
                     PTTLTIIQPDLGTGGLILIGGMLVVILAGLQFRVIFGLAAAGVAAGWLAWQYVLYDYQ
                     KQRVFTLLDPESDKLGAGYHIIQSQIAIGSGGVFGKGLLNGSQGQLEFLPERTTDFIY
                     AMVGEELGLLGASILLLLYVFVVGRALYLAVETQDTFARLLAGSIALTFFVYVFINAG
                     MVSGLLPVVGVPLPLVSYGGTSMVTLLASFGILMSLYSHRKLVAS"
BASE COUNT          750 a         1557 c         1409 g          828 t
ORIGIN      
        1 tttcgcttct tctgctacgc cctcctctcg gtcgtactca tgttctatga ccgtcgcggg
       61 caggagctgg gccgtggctg gctcgacacc gcgcgttatg gcctgcaggc ggctatctac
      121 ccgctgcagc tcgcggtcaa ctccccgtcc gccgcctggc gctggctcga agaaagtttc
      181 acgacgcgcg agaccctgcg cgccgaagtc gaccgcctgc gcgaagacct gcgcaaccag
      241 cagctggtca cgctgcggca gtcggcgctc gaaagggaaa acgccacatt gcgcggcctc
      301 aacgcgctgc tgccgaatgt catcgacaag cggctggtcg gcgaagtcat caacgtcgaa
      361 gtgtccacgc tgcgccagcg cctgctggtc aatcgcggcg gcacgcatgg tgtctacaag
      421 gcgcagccgg tggtcacggg tgacggcgtg ctgggccagg tgttccgcac cggcccgttc
      481 agttcggaga tcatcctcat caccgacgcg gagcacgcat tgcccgtgca ggtcgtccgc
      541 tccggcgttc gcaccatcgc cctcggcacc ggccgcagca cttcgctcga acttccctat
      601 gtaccgcaga actacgacgt gaaggtcggc gatctgctgg tcacctccgg cctcggccgc
      661 gtgttcccgt ttgggctgcc cgtcgcgcgc gtcacgaaag tggaacgcga tccgacgcag
      721 ccgctggcgc agatccacgc cgttcctctc gccggtatcg aaagcgatcg cgaagtgctg
      781 ttcatctggg aacgcaccga tcatcccgcc tctaccgcga ctcctgacgc cctggccgtc
      841 gaagcggccc caccggtgaa aaaaccatga acctcgcgcg tcgcgaaccg cgctgggcgg
      901 tcactttcac cgtgttgctg gcgctcatcg cgcaagtgct gccgctgccg gactggctgt
      961 cggtggtccg gccctcgttc gtcgcactgg tcatcatcta ctggagcatc tacgcgcccc
     1021 atgccggcgg catcttcgcg ccgtggctcg cgggtctcgc gctcgacgtg ttcaagggcg
     1081 aagtgctggc gcagaacgcg ctggccatcg cgctggtcgc ctacatcgcc atgagtctgc
     1141 accagcggct gcgcaaccag acgcttgtgc aacagtcgct gttcgtgttc gccgtgctca
     1201 cgctgaacga attcgtggtc tggggcatcg aaggatggag cgggcactcg gtgtccaccg
     1261 ggtggcgctg gattcagccg atggtcggcg caatgctgtg gccgttcatc gcgttgctgg
     1321 tcggccgcac tcacacacgt acctgacatg ggcacggtcc gcatcaagga tcactgggct
     1381 gagcagcgtc tcttcgagag ccgcgtgctc gccgccgcgt tcatcatcgc cggcctcacg
     1441 atcatcctca tcggccggct ggtcgtgctg caggtcatgc gctacgacta ctacgccgag
     1501 ctctcgcagg gcaatcgcgt gcgggtcgag ccgctgcccg cgcaacgcgg cctcatcctc
     1561 gatcgcaagg gcctggtgct ggcggaaaac cgtcccgcgt tccagctcga actggtgcgc
     1621 gaacgcgtgc ccgatgtcga agcgacgctg aagcggctca tcgaaattgg cgtgttgccg
     1681 gccgacgatc tcgacgaaac ccgcaggctg gtgcgttcgc gccgcagctt cgaaagcgtt
     1741 ccgatccggc tgcgcctcac ggacgaggaa atcgcccgct tcgccgtgca ccgtttcgaa
     1801 ttccccggcg tggacatcgc cacgcgcctc acgcgtttct atccgcacgg cgaacacgcc
     1861 gtgcacgcgc tcggctacgt tgcggcgatc agcgaaaacg acgtcaagcg tctcgagaag
     1921 gagaaggaac tctccaacta cgccggcacc acgctgatcg gcaaactcgg catcgaggcg
     1981 tcgtacgaaa agcagatgca tggcgtggac ggatcgcggc agatcctcgt caatgccgca
     2041 ggccggtcgg tgcagcgcca gggatccatg acgccggatt tgcaggagat caaaccggtc
     2101 gcgggccgcg acgtggttac cagcatcgac ctgccgaccc aactcgtggc ggaacaagga
     2161 ctcatcggcc gccgcggctc ggtggtcgcc atcgatccca gcaccggcga cgtcatcgcg
     2221 ctcgtctcga ctcccggctt cgatccgaac gctttcggcc gcggactcac caaggcggaa
     2281 tacgcgctgc tgcgcgacaa tatcgacatt ccgctgctga accgcgctat ccgcggtgaa
     2341 tatccacccg gctccaccgt caagccggtg atcgcgctgg cgggcctcgc caatggtgtg
     2401 acgacgcctc aacgcacgcg cgcctgcggt ggctacttca cgctgccggg ttcgcggcat
     2461 cgcttccgcg actggcgtcc tgcgggtcac ggcatcgtct ccatggaagc cgcgatcgcg
     2521 cagtcctgcg acgtctattt ctacgagctc gccaacatgc tcggcgtcga ccggctgtcg
     2581 gagttcctgt cgcacttcgg cctgggcgaa gtgaccggca tcgacatcag cggcgaacgt
     2641 aaaggcatcc tgccgtcgcc cgcgtggaag aaggcctact tcaagaccgc cgcgaaccag
     2701 gtgtggtttc cgggcgagac cgtgatcttc ggcatcggcc agggttacct catcgtgacg
     2761 ccgctgcagc tcgcgcacat gacctccatc gtcgcctcgc gcggcaagag ctaccggccg
     2821 cgcctggtca cggcgtatcg cgaacagtcc acgggcaaga tcgagccgat cccgcccaaa
     2881 ctactcgaga ccatcgacgt ggccacgccg gagaactggc agatcgcggt caacggcatg
     2941 atcagggtca tgagcggcga aggcggcacc ggcaggcgca gccaggtagg cgccgagtac
     3001 cagatcgccg gcaagacggg caccgcgcag gtgttcaccg tcaagcagaa cgagaactac
     3061 aaagcgatgg aagcgaggct caacgaacgt ctgctcgacc atggctggtt cgtcgcgttc
     3121 gcccctgcgg atgcgccgaa actcgccgtg gccgtgctca tcgaaaacgg caaacacggc
     3181 accgccgccg cgcccatcgc gcgccgcgtg ctcgaccagt acctgctcgg caggaccacc
     3241 acgccggaga ttccgcctca cgtggtcgcg cccacgggtg aagtcgtggt cgcgcccgac
     3301 gcgcccggag acgaatagtg gccatgtacg aacccatcga agtcggctcg cgcagccgcc
     3361 gcacgttcac cgccgcgggc cgcatcctgg gcgcgctcaa gctggatggt ccgctgctcg
     3421 tgggtctcgg gctcatcacg ctgtacggcc tcgtcgtgct ctacagtgcc tcgggccaga
     3481 actggaaccg cgtcatcgac ggcaccatcc gcgcggcact cggcgcggtc gccatgtgcg
     3541 cgctcgcgca ggtgaaaccc gcgttcctgc gccggctggc gctgttgatg tggatcgtgg
     3601 gcgtcgtgtt actcatcgtg gtcgacatca ccggccacat cggcaagggc gcacagcgct
     3661 ggctcgacct cggattcatc cgcttccagc catccgaaat catgaagctc gccgtgccca
     3721 tgacctgcgc gtggtggctg cacgatcggc cgctgccgcc ggactggaaa tcgttgctgg
     3781 cgctcgcggt catcatcctg gtgccgacga cgctcaccat catccagccc gacctcggca
     3841 ccggcggcct cattctcatc ggcggcatgc tggtggtgat cctcgccggt ctgcagttcc
     3901 gcgtgatctt cgggctcgcc gcggcaggcg tcgcagccgg ctggcttgca tggcaatacg
     3961 tgctttacga ctaccagaag cagcgcgtgt tcacgctgct cgacccggag tccgacaagc
     4021 tcggcgcggg ttaccacatc atccagtcgc agatcgccat cggctcgggc ggcgtgttcg
     4081 gaaagggcct gctcaatggc tcgcaaggcc agctcgagtt cttgccggag cgcacaacgg
     4141 acttcatcta cgccatggtc ggtgaagaac tcggcctgct cggagcctca atacttcttc
     4201 tgctgtacgt cttcgtcgta ggacgagccc tgtaccttgc ggtggaaacg caggacacat
     4261 tcgcccgatt gctggccggc agcattgcgc tcaccttctt cgtctacgtt ttcatcaatg
     4321 cgggcatggt cagcggcctt ctgccggttg tcggtgttcc gctaccgctg gtcagttacg
     4381 gcggaacatc gatggtgacc ctgctggcga gcttcggtat cctgatgtcc ctctactcgc
     4441 atcggaagct cgtcgcctcg tgatgcgcaa tgcgctcgca ctgacatcaa tcctcgtttt
     4501 ttcttcgggt tccgctctgg cggacggctc gttcgtcggc cgca
//
LOCUS       DATDEU010000273         2273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_48996, whole genome shotgun sequence.
ACCESSION   DATDEU010000273 DATDEU010000000
VERSION     DATDEU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48996"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VIV63_06660"
     CDS             complement(<1..507)
                     /locus_tag="VIV63_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY6124313.1"
                     /translation="MATPAPIEPVREFDLVPPDNSRLANLCGPLDENLRLIEDRMAVS
                     VKRRGSNFRISGARAHAAEDVVKVLFEATRHEDVSLERVHLALRDSRDMPGALGVHEP
                     ADDGGSVRVLRGTIRARGKNQKDYLTNIHTRDLSFGIGPAGTGKTYLAVACAVEALQA
                     DKIRRIILV"
     gene            complement(507..1877)
                     /gene="miaB"
                     /locus_tag="VIV63_06665"
     CDS             complement(507..1877)
                     /gene="miaB"
                     /locus_tag="VIV63_06665"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY6124314.1"
                     /translation="MPGRYYIKTFGCQMNEYDSTRMGDVLTAGADLTPTNDPAEADVL
                     LMNTCSVREKAEDKVYSLLGEWRALKKLKPHVLIGVGGCVASQEGEGITKRAPFVDLV
                     FGPQTLHRLPDMIGEKKRTGRAVVDVSFPEIEKFDNLPAPRAEGTSAYVSIMEGCSKY
                     CTFCVVPYTRGDEVSRSFDSVMDEVRSLASQGVGEITLLGQNVNAYAGAMDGTNIDLA
                     TLIHYVAEVDGLQRIRFTTSHPVEFGDSLIEAYANVPKLANQLHLAVQSGSDRILSAM
                     KRGYTALEFKEKLRRLRAVRPTISVSTDIIVGFPGETDRDFEATLKLVREANFDQSYT
                     FIYSQRPGTPAASLPDDVTLEVKQERLARLQDQLNSQARAISGQMVGSTQRVLVARPS
                     KKDAAELAGRTENGRWVNFAGPPGLIGHFVDVVIVEARPQSLRGRFMSATATTKPVGV
                     QRLAAN"
     gene            complement(1956..>2273)
                     /locus_tag="VIV63_06670"
     CDS             complement(1956..>2273)
                     /locus_tag="VIV63_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124315.1"
                     /translation="RSPLRSARGSLMELIDITIQRARRSERFAWAQYFTTAACIAYVI
                     AMYFSGIGDSMAAYHDAERATVALTIFALYGVGVGFYHYYARRRARRFIELRRALAPS
                     AAS"
BASE COUNT          397 a          726 c          742 g          408 t
ORIGIN      
        1 cacgaggatg atgcggcgga tcttgtcggc ctgcagcgct tcgaccgcgc atgccacggc
       61 gagataggtc ttgcccgtgc cggcgggacc gatgccgaac gacagatccc tggtgtggat
      121 gttggtgagg taatcctttt gattcttgcc acgcgcgcgg atcgtgccgc gaaggacgcg
      181 cactgaaccg ccatcgtcag cgggttcatg cacgccgagc gcgcccggca tgtcgcgcga
      241 atcgcgcagc gccagatgca cgcgctcgag cgatacgtct tcgtggcgcg tcgcctcgaa
      301 gagaaccttg accacatcct cggccgcgtg cgcacgcgcg ccggatatgc ggaagttcga
      361 gccgcgccgt ttcaccgaca cggccatgcg gtcttcgatc aggcgcaggt tttcgtcgag
      421 cgggccgcag aggttcgcga gccgcgaatt gtcgggcggc acgagatcga attcgcgcac
      481 cggttcgatg ggagcgggag tggccatcag ttggcggcga ggcgctgcac gcccacgggt
      541 ttcgtcgtag ccgtggcact catgaaacgg ccgcgcagcg attgcggccg cgcttcgacg
      601 atgaccacgt cgacgaaatg accgatgagc cccggcggac ccgcgaaatt cacccagcgg
      661 ccgttctcgg tccgccccgc gagttccgcg gcatcctttt tcgacgggcg cgcgaccaga
      721 acgcgctgcg tgctgccgac catctgcccg ctgatggcgc gcgcctggct gttcagctga
      781 tcctgcaggc gcgcgaggcg ttcctgcttc acttccagcg tgacgtcgtc cggcagcgaa
      841 gccgcggggg ttcccgggcg ctggctgtag atgaaggtat acgactggtc gaagttggct
      901 tcgcgcacga gtttcagtgt ggcctcgaag tcgcgatccg tctcgcctgg aaacccaacg
      961 atgatgtcag tggacaccga tatcgtcggg cggacggcac gcaggcgccg cagtttttcc
     1021 ttgaattcga gcgcggtgta gccgcgcttc atcgcgctga ggatgcggtc cgatcccgac
     1081 tgcactgcaa gatgaagctg gttcgcgagt ttcggcacgt tggcgtacgc ctcgatgagg
     1141 ctgtcgccga actccaccgg gtgcgaggtg gtgaagcgga tgcgctgcag cccgtccacc
     1201 tcggccacgt aatgaatgag cgtcgccagg tcgatgttcg tgccgtccat cgcgcccgca
     1261 tacgcattga cgttctggcc taacaaagtg atctcgccca cgccctgcga cgccagcgaa
     1321 cgcacctcgt ccatcaccga atcgaaggac cgcgacactt cgtcgccgcg cgtatacggc
     1381 accacgcaga acgtgcagta cttgctgcag ccttccatga tcgaaacgta tgcgctcgtg
     1441 ccctcggcgc gcggcgccgg caggttgtcg aacttctcga tctccgggaa ggacacatcg
     1501 accacggcgc ggccggtgcg cttcttctcg ccgatcatgt cgggcagtcg atgcagggtc
     1561 tgtgggccga acacgaggtc cacgaaaggc gcgcgtttgg tgatgccctc gccttcctgg
     1621 ctcgccacgc agccgcccac gccgatgagc acgtgcggct tgagtttctt gagcgcgcgc
     1681 cattcaccca gcagcgaata gaccttgtcc tcggctttct cacgaaccga gcacgtgttc
     1741 atgagcagca cgtcggcttc ggccgggtcg ttcgtcgggg tcaggtcggc accagcggtc
     1801 agcacgtcgc ccatgcgggt cgagtcgtac tcgttcatct ggcagccgaa agtcttgatg
     1861 tagtagcggc cgggcatcga ggagtcgagg ggaaaagcgg gcgctagggt atcgcagttc
     1921 gagtagccgc cagattactc cataaaaaca atggtttatg acgccgctga gggggccaaa
     1981 gcccgacgca actcgatgaa acggcgcgcc cggcggcgcg cgtagtagtg atagaaaccg
     2041 acgcccacgc catacagcgc gaagatggtc aacgccaccg tcgcccgttc cgcatcgtgg
     2101 tacgccgcca tcgagtcacc gatgcccgaa aaatacatgg cgatgacgta cgcgatgcac
     2161 gccgcggtgg tgaagtactg cgcccacgca aagcgttcgc tgcggcgcgc ccgctggatc
     2221 gtgatgtcga tgagctccat gaggctgccc ctggcgctgc gcagcggcga gcg
//
LOCUS       DATDEU010000274         3590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_779286, whole genome shotgun sequence.
ACCESSION   DATDEU010000274 DATDEU010000000
VERSION     DATDEU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779286"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1128)
                     /gene="ada"
                     /locus_tag="VIV63_06675"
     CDS             complement(46..1128)
                     /gene="ada"
                     /locus_tag="VIV63_06675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEY6124316.1"
                     /translation="MLHLMTPSPETTPSPGDDARWNAVQRRDHAADGTFVYSVRTTGV
                     YCRPSCAARLPRRENVAFHQSCADAERAGFRPCKRCRPNEPARADRQAAAVATACRLI
                     EEAEEVPSLDVLAQAADMSRFHFHRVFKSVTGVTPKAYAEAHRGNRVREELAARDTVT
                     QAIYGAGFNSGSRFYAASSGLLGMTPTQFRAGGSGNVIRFAVGECSLGAVLVAATDKG
                     VCAIDLGDDPDRLVRGLQDRFPNARLVGGDEAFEQLVAKVVGFVDAPAQGLDLPLDIR
                     GTAFQRRVWDAIRGIPAGSTASYAEIARRIGASKAVRSVAQACASNALAVAIPCHRVV
                     RTDGQLSGYRWGVARKRALLAREARA"
     gene            1443..>3590
                     /gene="leuS"
                     /locus_tag="VIV63_06680"
     CDS             1443..>3590
                     /gene="leuS"
                     /locus_tag="VIV63_06680"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY6124317.1"
                     /translation="MTTERYNAREAEPRWQKVWDQRNIFATRNDDPRQKYYVLEMFPY
                     PSGRIHMGHVRNYTMGDVVARYKRAIGLNVLHPMGWDAFGMPAENAAMERRVHPKAWT
                     YQNIAAMKAQLKSMGLSLDWAREIATCDPSYYKHQQKMFLDFLQAGLVERKLGKVNWD
                     PVDRTVLANEQVIDGRGWRSGALVEQREMPQWYFRITKFSEDLLQALDGLERWPEKVR
                     VMQRNWIGRSEGLHLRYALDPTTTPSGEDELDVFTTRHDTLFGAKFMALSPDHPLASA
                     AAAKNPALAEFIAECKRHGTAQQVIDTAEKQGFDTGMKAIHPFDPTWKLPVYVANFIL
                     MDYGTGAIFGCPAHDQRDLDFANKYGLGNTPVVCPPEQDPATFVITDTAYEGDGRMIN
                     SRFLDGMTIEQAKEEVARRLEHTSGGNRPVATRQVNFRLRDWGVSRQRYWGCPIPVIH
                     CEHCGIVPVPESDLPVTLPDDVDFDRPGNPLERHPTWKNVACPQCGGPARRETDTMDT
                     FVDSSWYFARFTDPWTESPTNRAVADRMMPVDQYIGGIEHAILHLLYSRFFTRAMRAT
                     GHIDLDEPFAGLFTQGMVIHETYQAANGEWVMPAEVKIEGAGEQRRATLRSTGEPVVI
                     GAIEKMSKSKRNTVDPDDIIDSYGADTARWFMLSDSPPERDVIWTEEGVQARWAFVQR
                     LWRLVREAGDVAAAPDSPRPNNFSEPALALRKTA"
BASE COUNT          668 a         1238 c         1117 g          567 t
ORIGIN      
        1 gcgcagaagc aggtgcacgc tcgcgttcag cggtctgtgc gcgggtcatg ctctcgcctc
       61 ccgcgccaat agagcccgct tgcgtgcaac gccccagcgg tagcccgaga gctggccgtc
      121 cgtgcgcacg acccgatgac acggaatggc caccgcaagc gcgttcgatg cgcacgcctg
      181 cgccaccgag cgcaccgcct tcgacgcgcc aatccgccga gcgatctcag catagcttgc
      241 ggtcgatccc gccgggatgc cgcggatggc gtcccatacg cgccgctgga aagcggtgcc
      301 gcgaatgtcg agcgggaggt cgagaccttg cgcgggggca tcgacgaagc ccaccacctt
      361 ggccacgagc tgctcgaagg cctcgtcgcc gccgaccagc cgcgcgttcg ggaaacgatc
      421 ctgcagaccg cgcaccagcc gatccggatc gtcgccgagg tcaatcgcgc agacgccctt
      481 gtccgtcgcg gcgaccagga ccgctccgag cgagcattcg ccgaccgcaa agcgaatgac
      541 gttgccgctg ccgccggcgc ggaattgcgt cggcgtcatg ccgagcaacc cggacgacgc
      601 ggcatagaag cggctgccgg aattgaagcc cgccccgtag atcgcctggg tgacggtgtc
      661 tcgcgcggcc agctcctcgc gcacgcgatt gccgcgatga gcttcggcgt aggccttggg
      721 cgtcaccccg gtgaccgact tgaacacccg atggaagtga aagcggctca tgtcagccgc
      781 ctgcgcgagc acgtcgaggc tgggcacctc ctccgcctct tcgatcagcc ggcacgccgt
      841 cgccacggca gcagcctgcc ggtcggcccg tgcgggctca ttcgggcggc agcgtttgca
      901 cggccgaaat cccgcccgtt cggcgtcggc gcagctctga tggaacgcga cattctcgcg
      961 ccggggcagg cgcgccgcgc acgacgggcg gcaataaaca ccggtcgtcc gcaccgaata
     1021 gacgaaggtg ccgtcggccg cgtgatcgcg ccgctgcact gcgttccagc gcgcgtcatc
     1081 gccgggcgag ggcgtcgttt ccgggctcgg tgtcatcaga tgcaacatcg ttttgctcca
     1141 tcaggttggc acggaacgca cggttccgcc tctgccggag caatctaggg atcgcaggac
     1201 tgcaagcact ccggtccttg ctctcaaact aaacataggc ccgccacccg gaaaccgcag
     1261 cccgcacgcc tgccggaccg gtcgatggtt ctgcagctcc ggccgttgac ccgtccggcg
     1321 ctttcatgtc caagtgcggc ccgaaaaccg gacgaaacgc acgatttatt agcgtcgaag
     1381 gcttatttct tgcgtctcgt tctttaaatc tcaagacctc aaggactcca gcggccgagc
     1441 caatgaccac cgaacgctac aacgcccgcg aggcggagcc gcgttggcag aaggtgtggg
     1501 accagcgaaa catcttcgcc acccgcaacg acgacccgcg ccagaaatac tacgtgctcg
     1561 agatgttccc ctatccgtcg gggcgcatcc atatggggca cgtgcgcaat tacaccatgg
     1621 gcgacgtggt cgcgcgctac aagcgcgcga tcggtctcaa cgtgctccat cccatgggct
     1681 gggacgcctt cggcatgccg gcggagaacg ccgccatgga gcgcagggtt catcccaagg
     1741 catggaccta ccagaacatc gcggcgatga aggcgcagct caagtcgatg ggactgtcgc
     1801 tggactgggc acgtgaaatc gcgacctgcg atccgtccta ctacaagcat cagcagaaga
     1861 tgttcctcga cttcctccag gcgggactgg tcgagcgcaa gctcggcaag gtgaactggg
     1921 acccggtcga tcgcaccgtg cttgccaacg agcaggtgat cgacgggcgc ggatggcggt
     1981 cgggcgcatt ggtcgaacag cgggagatgc cgcaatggta tttccgcatc accaaatttt
     2041 ccgaagatct ccttcaggcg ctcgacggcc tcgagcgctg gccggagaag gtgcgcgtca
     2101 tgcagcgcaa ctggatcggc cgctcggaag gattgcacct gcgctacgcg ctcgacccca
     2161 cgaccacccc gtcgggagaa gacgagctcg acgtcttcac cacacgccac gacaccttgt
     2221 tcggcgccaa gttcatggcg ctctcgcccg atcatccgct cgccagcgct gcggcggcca
     2281 agaatcctgc gcttgccgaa ttcatcgcgg aatgcaaacg ccatgggacc gcgcagcagg
     2341 tcatcgacac cgccgagaag cagggttttg acaccgggat gaaggcgatc catcccttcg
     2401 atccgacctg gaagctcccg gtctatgtcg cgaatttcat cctgatggac tacggcaccg
     2461 gcgccatctt cggctgcccg gcacacgatc aacgcgatct cgatttcgcc aataaatacg
     2521 ggctcggcaa cacccccgtg gtctgcccgc cggagcagga tccggcgacc ttcgtcatca
     2581 ccgataccgc ctatgaaggc gacggccgca tgatcaactc gcgctttctc gacggcatga
     2641 ccatcgagca agcgaaggag gaggtggcgc gacggctcga gcacacgtcc ggcggaaatc
     2701 gcccggtcgc cacgcgccaa gtcaatttcc gcctgcgcga ctggggcgtt tcccgccagc
     2761 gctactgggg ctgcccgatc ccggtgatcc actgcgagca ctgcggcatc gtgccggtgc
     2821 cggagagcga tctacccgtg acgttgccgg atgacgtcga cttcgaccgg ccaggcaatc
     2881 cgctcgagcg ccatcccacc tggaagaacg tcgcgtgtcc gcaatgcggt gggccggcac
     2941 gccgcgaaac cgacaccatg gacacgttcg tcgactcgtc ctggtacttc gcacgcttca
     3001 ccgacccgtg gaccgagagt ccgaccaatc gcgcggtcgc agaccgcatg atgcctgtcg
     3061 atcagtatat cggcgggatc gagcatgcga tcctgcacct gctctacagc cggttcttca
     3121 cccgcgcgat gcgtgccacc gggcacatcg atctcgacga accgttcgcc ggccttttca
     3181 cccagggcat ggtgatccac gagacctacc aagccgccaa cggcgaatgg gtcatgccag
     3241 ccgaagtgaa gatcgaaggc gcgggcgagc agcgccgcgc cacccttcgc agcaccggcg
     3301 aaccggtcgt gatcggcgcg atcgagaaaa tgtcgaagtc gaaacgcaac accgtcgacc
     3361 ctgacgacat catcgacagc tacggcgccg acaccgcgcg ctggttcatg ctctcggact
     3421 caccgcccga gcgggacgtg atctggaccg aggaaggcgt tcaggctcgc tgggcgttcg
     3481 tgcagcggct ctggcgcctg gtgcgggagg cgggcgacgt ggccgccgcc cccgattcgc
     3541 cgcggccaaa caacttcagc gaaccggcgc tcgccctgcg caagaccgcc
//
LOCUS       DATDEU010000275         2974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_633562, whole genome shotgun sequence.
ACCESSION   DATDEU010000275 DATDEU010000000
VERSION     DATDEU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633562"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VIV63_06685"
     CDS             <1..995
                     /locus_tag="VIV63_06685"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124318.1"
                     /translation="IVLSGQAERESIMRALPVTHQFLHKPCDGETLRAVIEQTHALHK
                     LMQNPALTALVGKLERIPAVPSTYTELARLAADPKADSASFAKVIEMDAAVCAKVLQL
                     VNSAYFGLGQKIVSIRPAVTYLGVEIIKSLVLGSTSFSDKAISEVKGFSPDRLQHHSM
                     LTAVLAKKIVSNPSLADAAFTAGLLHDIGSLVLLHAAPPDYVRSLERKKELNGDSAAA
                     EREIFGVTHAEVGAYLLGLWGIPFPIVEAVAFHHRPNEVAPESRPLVAAIHIASGLID
                     EMTESDATKRPPGDSIDSSSRIDVSFLREAGLEEAFKKNRAEAEKLLGNVSDRS"
     gene            1051..2121
                     /locus_tag="VIV63_06690"
     CDS             1051..2121
                     /locus_tag="VIV63_06690"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEY6124319.1"
                     /translation="MRIARIAAVGLSLAAGAAGALLVSRPDDARAADSPRSPAAATAA
                     NAFLKSLPADLRAIASFPLESPQRTTWNFVPMDRAGVSLLKLDDSQSELLGPLLATAL
                     SPEGLLAARGVMKHENILRRVETEAGVGNASRRDPGLYYTSVFGKPAAGGAWGWRFEG
                     HHLSVNVTELPGEAPIVAPMFVGANPARVLDGPNAGFRLLAAEEDLGRELIKMLPASR
                     RATATIRDTAFAEILTGNDPKVQGLELAGLAAADMTAEEKTQLRRLIELYAGRMAPAS
                     ARDALMRLDRAGFEKVRFAWAGGIEPGQPHYYRVHGPTMLIEYDDTQNNANHIHTVYR
                     DLERDFGGDVLRAHYRGDLSKP"
     gene            2188..2700
                     /locus_tag="VIV63_06695"
     CDS             2188..2700
                     /locus_tag="VIV63_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEY6124320.1"
                     /translation="MGKKNTAASVPTMAAPLIDIGIKGKDREKIVAGLSSVLADTYTL
                     YLKTHNFHWNVEGPMFNTLHLMFMDQYTELWNALDAIAERIRSLGYPAPGTGKEFSKL
                     TSIAETEGVPDALEMVRLLVAGHEAVTRTARAAFPAAEKAGDESSCDLLTQRLQIHEK
                     TAWMLRSLLK"
     gene            complement(2728..>2974)
                     /locus_tag="VIV63_06700"
     CDS             complement(2728..>2974)
                     /locus_tag="VIV63_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124321.1"
                     /translation="SGLRSSLSALTKPYEGGMWCFFKTLMVSRAKSDCRLPSGSVPLS
                     GRSSKVSATLSFDSAAATEQNSASAATSRNFRCGPFC"
BASE COUNT          554 a         1023 c          925 g          472 t
ORIGIN      
        1 gtatcgtgct gtccggccag gccgagcgcg aatccatcat gcgcgcgctg cccgtgaccc
       61 accagttcct gcacaagccc tgcgacggcg aaacgctgcg cgcggtcatc gagcagaccc
      121 atgcgctgca caagctcatg cagaacccgg cgctgaccgc gctcgtgggc aagctcgaac
      181 gtattcccgc ggtgccgtcg acgtacaccg aactcgcgcg cctcgcggcc gacccgaagg
      241 ccgacagcgc ttcgttcgcc aaggtcatcg agatggacgc cgcggtttgc gccaaggtgc
      301 ttcagctcgt gaactcggcg tacttcggcc tgggtcagaa gatcgtcagc atccggcccg
      361 ccgtgactta cctcggtgtc gagatcatca agtcgctggt gctcggctcc acgtcgttct
      421 ccgacaaggc catcagcgaa gtgaaaggct tctcgcccga ccgcctgcag caccactcga
      481 tgctcacggc cgtgctcgcc aagaagatcg tgtcgaatcc gtcgctggcc gatgcggcgt
      541 tcaccgccgg cctgctgcac gacatcggct cgctggtcct gctgcatgcc gccccgcccg
      601 actacgtgcg atcgctcgaa cgcaagaaag agctcaacgg cgacagcgcc gcggcggagc
      661 gcgagatctt cggcgtcacc cacgccgagg tcggcgccta tctactgggc ctgtggggca
      721 tccccttccc catcgtcgag gcagtcgcgt tccatcaccg gcccaacgaa gtggcgccgg
      781 aatcgcgtcc gctggtcgcg gcgatccaca tcgcgagcgg cctcatcgac gagatgaccg
      841 agagcgacgc cacgaaacgc ccgccgggcg acagcatcga cagctcgagc cgcatcgacg
      901 tcagcttcct gcgcgaagcc gggctcgaag aggcgttcaa gaagaaccgc gccgaggcgg
      961 aaaagctgct cggcaacgtc tcggatcgaa gctagccaac gcgtcacgcg tacggatagg
     1021 atcgcgctcc ccggggggaa ggagagcgcg atgcgtattg cccgaattgc agcggtcgga
     1081 ctgtcactgg ccgcaggcgc cgcgggggcg ttgctcgtct cgcgcccgga cgacgcgcgc
     1141 gccgccgata gccccaggag tccggccgcg gccaccgccg ccaacgcttt cctgaagtca
     1201 ctccccgctg accttcgcgc catcgccagt tttccgctgg agtccccgca gcgcacgacc
     1261 tggaatttcg tgcccatgga tcgcgccggc gtctcgctgc tgaaactcga cgattcgcag
     1321 agcgaactgc tgggcccgtt gttagccacg gcgctcagcc ccgaaggtct gctcgcggct
     1381 cgcggcgtca tgaaacacga gaacatcctg cgccgcgtcg agaccgaggc cggcgtgggc
     1441 aacgcgtcac gtcgcgatcc cggtctctac tacaccagcg tcttcggcaa gccggcggcg
     1501 ggcggcgcct ggggctggcg attcgaaggg catcacctgt ccgtcaacgt cacggagctg
     1561 cccggcgagg cgcccatcgt ggcgcccatg ttcgtgggcg cgaaccccgc gcgcgtgctc
     1621 gacgggccca acgccggatt ccgcttgttg gccgccgaag aggatctggg ccgcgaactg
     1681 ataaagatgc tgcccgcctc gcgccgcgcc acggctacga ttcgcgacac cgccttcgcc
     1741 gagatcctta ccggcaacga tcccaaggtg cagggcctcg agctcgccgg actcgccgcg
     1801 gcggacatga cggccgaaga gaagacgcag ttgcgccgcc tcatagagtt gtatgcggga
     1861 cgcatggcgc ccgcgtcggc cagggatgcg ttgatgcgcc tggatcgggc aggcttcgag
     1921 aaagtgcgct ttgcatgggc aggtggcatc gaaccgggcc agccgcatta ctaccgcgtg
     1981 cacggtccca cgatgctcat cgaatatgac gatacgcaga acaacgccaa ccacattcac
     2041 accgtctatc gcgatctcga gcgcgacttc ggcggtgacg tgttgcgggc acactatcga
     2101 ggcgacctat caaagccata ggcatttgcc attggcaggg cgcgcgaggc atggctagac
     2161 tcgccgcatc gcgtgaggag taacgacatg ggcaagaaga acaccgccgc cagcgtcccg
     2221 acgatggccg ctccgctgat cgacatcggc atcaagggca aggaccgcga gaagatcgtc
     2281 gccggcctgt cgagcgtgct cgccgacacg tacacgctgt atctcaagac tcacaacttc
     2341 cactggaacg tggaaggccc catgttcaac acgctgcatc tcatgttcat ggaccagtac
     2401 accgagttgt ggaacgcact cgatgcgatt gccgagcgaa tccggtcgct gggttacccc
     2461 gcgccgggca caggcaagga attctcgaag ctcacgtcca tcgcggagac cgaaggcgtg
     2521 cccgacgcgc tggagatggt gcggttgtta gtcgccggtc atgaagccgt aacgcgcacg
     2581 gcgcgcgccg ccttccccgc cgccgaaaag gccggcgatg aatcgagctg cgacctgctg
     2641 acgcagcgcc tgcagattca cgaaaagacc gcctggatgc tccgcagcct cctgaagtga
     2701 aaccgaaatg gggacactcc ccatttgcta gcaaaagggg ccgcacctga aattcctgct
     2761 cgtcgccgcg ctcgcgctgt tctgttccgt cgcggccgcg gagtcgaagc tcaaggtcgc
     2821 gctgaccttc gacgacctgc cgctcaatgg cacgctgcca ctcggcaagc ggcagtccga
     2881 cttcgcgcgc gacaccatca aggtcttgaa gaagcaccac atcccgccct cgtacggctt
     2941 cgtcaatgcc gacaagctcg agcgcaatcc ggat
//
LOCUS       DATDEU010000276         3385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1800424, whole genome shotgun sequence.
ACCESSION   DATDEU010000276 DATDEU010000000
VERSION     DATDEU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800424"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VIV63_06705"
     CDS             <1..928
                     /locus_tag="VIV63_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6124322.1"
                     /translation="ISSVELEDGQQVRGDLFIDCSGFRGLLIEQTLKAGYDDWSHWLP
                     ANRAVAVPCAKSGEMTPYTRATAREAGWQWRIPLQHRTGNGHVYASSFTTDERARELL
                     MANLDAPALAEPRLIKFTTGRRKQLWNRNVVCMGLASGFLEPLESTSIHLIQVTIQRL
                     ILHFPHLGNVEERRREFNRAAAAEYDYIRDFIILHYHANNRVGEPFWDACRNMSIPDS
                     LRHRIELFRECAGIFCATDDLFQLTSWLQVLWGQGVRPQATHAFVEAVAPKDRAGYLD
                     DLRQLYSRAAKALPGHAEFIARHCAATPANAA"
     gene            complement(930..>3385)
                     /gene="pulA"
                     /locus_tag="VIV63_06710"
     CDS             complement(930..>3385)
                     /gene="pulA"
                     /locus_tag="VIV63_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA];
                     GO_process: GO:0005983 - starch catabolic process
                     [Evidence IEA];
                     GO_process: GO:0051678 - pullulan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pullulanase-type alpha-1,6-glucosidase"
                     /protein_id="HEY6124323.1"
                     /translation="LRARFPHLANLAAFKLPADAVAAAAQSLKGQLAVGAARPGVGVD
                     ATSLQIPGVLDDLYAANAGPVTLGPSFKHGQTTLRVWAPTARSLAVRVFADSATADFT
                     THPMTLDSASGIWSYTAPTGSVHGKFYLYEAQVYVRSTNRVETNIVTDPYSVSLARNS
                     TRSQIVSLDDAALQPRGWNKLNKPWLAAPEDIVLYELHVRDFSATDTTVPENLRGTYS
                     AFTQRRSDGMKHLATLGLAGVTHVHLLPAFDFATTNEDKSAWQAPSFDTLAGFPADSP
                     EQQALVAATSDRDAFNWGYDTLHYNVPEGSYATDADGGARVREFREMVQSLNQTGLRV
                     VMDVVYNHTNSAGQNPNSILDKLVPGYYHRLNGDGIVLGESCCADTATEHAMMEKLMV
                     DSVVLWAKAYKVDGFRFDIMGFHLKSNMLKVRDALHALTLARDGVDGKSIYLYGEAWN
                     FGVMANNGRGENAIQVNMAGTGIGSFSDRIRDAVRGGGPFSPRRDQGFATGLYVEPNG
                     TFGGSAADAKTELFRLTDLVRLGLAGNIASYEITDGSGVTRPGSEVDYFGMPGAYGAQ
                     PEDTIQYIGVHDDPDWFDAMNLKLSTTVPRADRVRMHRLGLSVVALSQGVPFFMAGDE
                     ILRSKSADRNSYNSGDWFNRIDWTLKSNNWGVGLPPAQGNQDDWSIIGPALADPNLKP
                     NAKDIEGTYRHMLEMLVIRKTSKLFRLRTADEIKKAVSFYNVGPSQTPGLIVERIRNT
                     DKFCLGASEAVVLVNATPQTQAFTDAAFRKEPLLLHPIQALSRDPVVRTSKFQRSTGT
                     FTIPARTTAVFIEPCFGPH"
BASE COUNT          553 a         1081 c         1146 g          605 t
ORIGIN      
        1 catctcgtcg gtcgaactgg aggacggcca gcaggttcgc ggcgacctgt tcatagactg
       61 cagcggcttc cgcggcctgt tgatcgagca gacattgaag gccggctacg acgactggag
      121 ccactggctg ccggccaacc gcgccgtcgc cgtgccctgc gccaagtcgg gggagatgac
      181 gccctacacc cgcgccaccg cgcgcgaggc tggctggcag tggcgcatcc cgctgcagca
      241 ccgcaccggc aatggtcatg tctacgcgtc gtcattcacc accgacgaac gcgcgcgcga
      301 gttgctgatg gcgaacctcg atgcgccggc gctcgcggag ccgcggctca tcaagttcac
      361 gacgggcagg cgcaagcagc tctggaaccg caacgtggtg tgcatggggc tcgccagcgg
      421 attcctcgag ccgctcgaat ccacgtccat ccacctgatc caggtgacga tccagcggct
      481 gatcctgcat ttcccgcatc tcgggaacgt cgaggagcgg cgccgggaat tcaaccgcgc
      541 ggcggcggcg gaatacgact acatccgcga cttcatcatc ctgcactacc acgcgaacaa
      601 ccgcgtgggc gagccgttct gggacgcgtg ccggaacatg tcgattcccg attcgctgcg
      661 tcatcgcatc gagctgtttc gcgaatgcgc cggcatcttc tgcgcgaccg acgacctgtt
      721 ccagctcacg agctggctgc aggtcctgtg ggggcagggc gtgcggccgc aagcgacgca
      781 tgcgttcgtc gaggcagtcg cgcccaagga tcgcgcgggt tacctcgacg atctgcgcca
      841 gttgtattcg cgggcagcga aggcgttgcc gggccacgcc gagttcatcg ccaggcactg
      901 cgccgccaca cccgccaacg ctgcctgagt cagtgcggac cgaagcaggg ttcgatgaag
      961 accgccgtcg tgcgcgccgg gatcgtgaac gtgccggtgc ttcgctggaa cttcgacgtg
     1021 cggaccaccg gatcccgcga aagcgcctgg atgggatgca gcaacagcgg ttccttgcgg
     1081 aacgcggcat ccgtgaatgc ctgcgtctgc ggcgtggcat tcaccagcac gacggcttcg
     1141 gacgcgccca ggcagaactt gtcggtgttc cggatgcgct cgacgatgag ccccggcgtc
     1201 tgtgaaggac cgacgttgta gaaactcacg gccttcttga tctcgtcggc cgttcgcagc
     1261 cggaagagtt tgctggtctt gcgaatcacg agcatctcca gcatgtggcg gtacgtgccc
     1321 tcgatgtcct tcgcgttggg cttgaggttc ggatcggcga gcgcgggccc gatgatcgac
     1381 cagtcgtcct ggttgccctg cgccggcggc aggccgacgc cccagttgtt ggacttcagc
     1441 gtccagtcga tgcggttgaa ccagtcaccc gagttgtagc tgttccggtc cgccgatttg
     1501 gaccgcagga tttcgtcgcc ggccatgaag aagggcacgc cttgcgatag cgccacgacc
     1561 gacagtccca ggcggtgcat gcgcacgcgg tcggcgcgcg gcacggtggt ggagagcttc
     1621 aggttcatgg cgtcgaacca gtccggatcg tcgtgcacgc cgatgtactg aatcgtgtct
     1681 tcgggctgcg cgccgtaggc gccgggcatg ccgaagtagt cgacctcgct gccgggcctg
     1741 gtcacgccgc tgccgtccgt gatctcgtag ctcgcgatat tgccggcgag ccccagtcgc
     1801 acgaggtcgg tgagacggaa caattcggtc ttcgcgtccg ccgcggaacc accgaaagtg
     1861 ccattgggtt cgacgtacag gcccgtcgcg aagccctggt cgcgccgcgg gctgaacggt
     1921 ccgccgccgc gcaccgcgtc acggatgcga tcggagaacg agccgatgcc ggtgccggcc
     1981 atgttcacct ggatcgcgtt ctcgccgcgg ccattgttcg ccatgacgcc gaagttccag
     2041 gcctcgccgt atagatagat ggacttgccg tcgacgccgt ccctggccag cgtgagtgcg
     2101 tgcaacgcat cgcgcacttt cagcatgttg gatttgaggt gaaagcccat gatgtcgaag
     2161 cggaagccgt cgaccttgta tgccttggcc cacaacacga ccgagtcgac catgagcttt
     2221 tccatcatcg cgtgttcggt cgccgtgtcg gcgcagcagc tttcgccgag cacgatgcca
     2281 tcgccgttca gccggtggta ataaccgggc accagtttgt cgaggatgga gttcggattc
     2341 tggcccgccg aattggtgtg gttgtacacc acgtccatga cgacgcgcag gccggtctgg
     2401 ttgagcgact gcaccatctc gcggaactcg cgcacgcggg cgccgccatc ggcgtcggtc
     2461 gcgtagctgc cctcgggcac gttgtagtgc agcgtgtcgt aaccccagtt gaaggcatcc
     2521 cgatccgacg tggccgcgac cagcgcctgc tgttcgggag aatcggcggg gaagcccgcc
     2581 agcgtgtcga acgaaggcgc ctgccatgcg gacttgtctt cgttggtggt cgcgaagtcg
     2641 aacgcgggca agaggtgcac gtgcgtcacg ccggcgagtc ccagcgtcgc caggtgcttc
     2701 atgccgtccg agcgccgctg cgtgaatgcc gaataggtgc cgcgcaggtt ctcgggaacc
     2761 gtggtgtcgg tggcgctgaa gtcgcgcaca tgcagctcgt acagcacgat gtcttcgggc
     2821 gcggcgagcc agggcttgtt caacttgttc cagccgcgcg gctgcagagc cgcatcgtcg
     2881 agcgacacga tctggctgcg cgtgctgttg cgcgccagcg agaccgaata gggatcggtg
     2941 acgatgttgg tctcgacgcg gttggtcgaa cgcacgtaga cctgcgcttc gtacagatag
     3001 aacttgccgt ggacactgcc cgtcggcgcg gtgtagctcc agatgccgct tgcggagtcg
     3061 agcgtcatgg gatgcgtcgt gaaatccgcg gtcgccgagt ccgcgaagac gcgaaccgcg
     3121 aggctgcggg cggttggcgc ccagacgcgc agggtcgtct gtccgtgttt gaagctgggt
     3181 ccgagcgtga ccggccccgc gttggcggcg tacaggtcgt cgagaacacc cgggatctgc
     3241 agcgacgtcg catcgacgcc cacgccgggc cgcgccgcac cgacggcgag ctggcctttg
     3301 aggctttgag cggctgccgc gacggcatcg gcaggcagct tgaaggccgc cagattcgcc
     3361 agatgcggaa atcgggcgcg cagcg
//
LOCUS       DATDEU010000277         4614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_340795, whole genome shotgun sequence.
ACCESSION   DATDEU010000277 DATDEU010000000
VERSION     DATDEU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340795"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2647
                     /locus_tag="VIV63_06715"
     CDS             <1..2647
                     /locus_tag="VIV63_06715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY6124324.1"
                     /translation="AGRAWLAVELTRAGRDRQSRAHGRELAAALNLPLTAAGDVHMHV
                     RERRRLQDALTAVRHGVSVAEAGTRLHPNGERHLRDRARLARLYPPELLAETVKIAAL
                     AHFSLDELRYQYPQELVPAGETAASHLRKLTEAGARKRWPAGVPQKMRAQIDYELKLI
                     NDLSYESYFLTVQDIVSEARRLSILCQGRGSAANSVVCYALGVTEVDPSRGELLMERF
                     ISRERNEPPDIDIDFEHERREEVIQYIYRKYGRDRTALTATVISYRPRSALRDMAKVM
                     GLDAVQAERLAGAMQWWDTAVDEDRIREAGFEPGNPKLKMLLDLAGQLQGFPRHLSQH
                     VGGFVISQGLLEELVPIENAAMADRTVIEWDKDDLDELGLLKVDVLGLGMLSALRRGL
                     ALVSDFRRKPFTLADVPKEDARVYDMISRADTIGVFQVESRAQMSMLPRLKPKEFYDL
                     VIEVAIVRPGPIQGGMVHPYLRRRQGLEPVTYPSDAVKGVLSRTLGVPIFQEQVMQLA
                     IVAAGFTPGEADQLRRSMAAWKRRGGLGHFEQRLIDGMRHRGYDESFARQIFSQIQGF
                     GEYGFPESHAASFALLVYSSAWLKRHEPAAFTCALINSQPMGFYAPSQLVRDARDHGV
                     EVRPIDVCVSDWDCTLERQPDGEPALRLGLRLVKGLSEEATDRIVTARAQTQFASVQD
                     LTLRADLARRDLESLADAGALRELSGNRHLTFWQVAGSEQELPLAPVPQSAEGTPLLA
                     RPTEGQNIASDYRTLGLTLGRHPLALLREELMTAHIVTSADLRDLPHGRVVRVAGIVT
                     ARQRPQSAGGVMFITLEDETGYVNLIVWERVWSRARRIASGSRMLEVAGMLQKEGLVT
                     HVVAQRLFDRTGMLGTIVTQSRDFR"
     gene            complement(2657..3076)
                     /locus_tag="VIV63_06720"
     CDS             complement(2657..3076)
                     /locus_tag="VIV63_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6124325.1"
                     /translation="MTTDAPPRKPIVLGPGEGRAYPMGRIDAVFKADGAETGAGYSIS
                     EWWLEPHTRGPGAHSHPEDDIFYVIAGTMSVLVGNEWKDAAPGSFVLIPGGVTHDFEN
                     RGEVRAGVLNFYAPGTFEEHMPEIARWWRENPPGNTD"
     gene            complement(3136..3408)
                     /locus_tag="VIV63_06725"
     CDS             complement(3136..3408)
                     /locus_tag="VIV63_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124326.1"
                     /translation="MNEKPESEEFDEDEEEVAFEATSEVDFDRLAKDLDAAKKRGQKL
                     GDPAWRRLERRFEDKHTAELTSDFDDYNIDDDSTAVRKMNNTKNAS"
     gene            complement(3490..>4614)
                     /gene="gspF"
                     /locus_tag="VIV63_06730"
     CDS             complement(3490..>4614)
                     /gene="gspF"
                     /locus_tag="VIV63_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY6124327.1"
                     /translation="LLRERQLLPVTVAEVKHGEAKRDRSFTVRRGVSASDLSLLTRQL
                     ATLVKAGLPLEEALLAVSQQSEKPRVQSIMLGVRGKVMEGHTLADGVAEFPKVFPEIY
                     RSTVAAGESSGRLDGILERLADYTENREVTRQKVLGAMLYPIVLSIICLGIVTGMMVY
                     VVPKVIEVFESSKGKLPFATQVLIAVSEFLRNWGIYLALGIIGGSIFFVYWLKNPDNR
                     RRFHRFQLRLPLFGRLVRGFNTARFTRTFSILTASAVPVLEAMRIAGEVVTNLPMRDA
                     VAAAAARVREGAPIGKSLAHSKLFPPMTVHLISSGESSGELDTMLERAATNQERELDS
                     MMGALVGLLGPLLIVVMGMFVMGIVFAMLMPIFEMNNLIR"
BASE COUNT          801 a         1498 c         1506 g          809 t
ORIGIN      
        1 cgccggccgc gcctggctcg ccgtggagct cacgcgcgcc ggccgcgatc gccaaagtcg
       61 ggcgcatggc cgcgagcttg ccgcggcgct gaacctgccg ctgaccgccg ccggcgacgt
      121 gcacatgcac gtgcgcgagc ggcgccgcct gcaggatgcg ctcacggcgg tgcgtcacgg
      181 tgtctcggtc gccgaggccg gcacgcggct gcatccgaac ggcgaacgtc acctgcgtga
      241 tcgcgcacgt ctcgcgcggc tctatccacc cgagctgctg gcggaaaccg tgaagatcgc
      301 cgcgctcgcc cacttctcgc tcgatgaact gcgttatcaa tatccgcagg aactggtgcc
      361 ggccggagaa acggcggcct cgcacttgcg caaactcacg gaagccggcg cgcgcaaacg
      421 ctggcccgcg ggtgttccgc agaagatgcg cgcgcagatc gactacgaac tgaaactcat
      481 caacgacctc tcttacgaat cctatttcct cacggtgcag gacatcgtga gcgaggcgcg
      541 ccgcctcagc atcctctgcc agggccgcgg ttccgccgcc aactcggtgg tgtgttatgc
      601 gctgggagtc accgaggtgg atccgtcgcg cggcgaactg ctcatggagc gtttcatctc
      661 gcgcgagcgc aacgaaccgc ccgacatcga catcgacttc gaacacgagc ggcgcgaaga
      721 agtcatccag tacatctacc gcaagtacgg ccgcgatcgc accgcgctca ccgccaccgt
      781 tatcagctac cggccgcgca gcgcgctgcg cgacatggcc aaggtgatgg ggctcgacgc
      841 ggtccaggcc gagcgtctcg caggcgccat gcaatggtgg gacaccgcgg tcgacgaaga
      901 ccgcatacgc gaagcgggct tcgagcccgg caatccgaag ctgaagatgt tgctcgacct
      961 ggcaggccag cttcagggat tcccgcggca cctctcgcaa cacgtgggcg gcttcgtgat
     1021 ctcgcaaggc ctgctggaag aactcgtgcc catcgaaaac gccgccatgg ccgatcgcac
     1081 cgtcatcgaa tgggacaagg acgacctcga cgaactcggc ctgctgaagg tggacgtgct
     1141 ggggctcggc atgttgtcgg cgctgcgccg cggtctcgcg ctggtgtcgg acttccggcg
     1201 caagccgttc acgctcgcgg atgtgcccaa ggaagatgcg cgcgtctacg acatgatttc
     1261 gcgcgccgac accatcggcg tcttccaggt cgaatcgcgc gcgcagatgt ccatgctgcc
     1321 gcgtctcaag cccaaggagt tctacgacct cgtcatcgaa gtcgccatcg tgcgcccggg
     1381 tcccatccag ggcggcatgg tccatcccta cctgcgccgg cggcagggcc tcgagccggt
     1441 cacctatccc agcgacgcgg tgaagggtgt gctctcgcgc accttagggg tgcctatctt
     1501 ccaggagcag gtgatgcagc tcgccatcgt cgcggccggt ttcacgccgg gcgaagccga
     1561 ccagttgcgc cggagcatgg cggcctggaa gcggcgaggg ggtctcgggc atttcgagca
     1621 gcggctcatc gacggcatgc gccatcgcgg ctacgacgaa agcttcgcgc gccagatctt
     1681 cagccagatc cagggtttcg gcgaatacgg ctttcccgag tcgcatgccg cgagtttcgc
     1741 gctgctggtg tacagctccg cgtggctcaa gcggcatgaa ccggcggcgt tcacctgcgc
     1801 gctcatcaac agccagccca tggggtttta tgcgccgtct caactggtgc gcgatgcgcg
     1861 cgatcacggc gtcgaagtcc gtcccatcga tgtctgcgtc agcgactggg attgcacgct
     1921 cgaacgccag cccgacggcg agccggcgtt gcggctgggt ctgcgcctgg tgaaaggcct
     1981 gagcgaggag gccaccgatc gcatcgtcac ggcgcgtgcg caaacacagt tcgcgagcgt
     2041 ccaggacctc acgctgcgcg ccgacctggc gcgccgcgac ctcgaatcgc tggcggatgc
     2101 cggcgcgctg cgcgaactgt caggaaaccg tcatctcacg ttctggcagg tggccggcag
     2161 cgaacaggag ctgccgctcg cgcccgtccc gcaatccgcc gaaggcacgc ctctgctcgc
     2221 gcggccgacc gaggggcaga acatcgcctc cgactaccgc accctgggcc tcacgctggg
     2281 gcgccatccg ctggcgttgc tgcgcgagga gctcatgacg gcccacatcg tcacctcagc
     2341 cgatctgcgc gacctgccgc atgggcgcgt ggtccgtgtg gcgggcatcg tcacggcgcg
     2401 ccagcgcccg cagagcgccg gtggtgtcat gttcatcacg ctcgaggacg aaaccggcta
     2461 cgtgaacctc atcgtctggg aacgcgtgtg gtcgagggcg cggcgcatcg ccagcggatc
     2521 gcgcatgctc gaagtcgccg gcatgctgca gaaagagggg ctggtcactc acgtggtcgc
     2581 gcagaggctg ttcgaccgga cgggcatgct cgggacgatc gtcacgcaat cgcgcgactt
     2641 cagatgagtc cgtgtatcag tccgtgttgc cggggggatt ttcccgccac cagcgcgcga
     2701 tctcgggcat gtgttcttcg aacgtgcccg gtgcatagaa attgagcacg ccggcgcgca
     2761 cctcgccgcg gttctcgaag tcgtgcgtca cgccgccggg gatgagcacg aacgaacccg
     2821 gcgccgcgtc cttccactcg ttgcccacga gcacgctcat ggtccccgcg atcacataga
     2881 agatgtcgtc ttcaggatgt gaatgcgcgc ctgggcccct ggtgtgcggc tcgagccacc
     2941 actcggagat ggaataaccc gcgccggtct cggcgccatc ggctttgaac accgcatcga
     3001 tgcggcccat gggataggcg cggccttcgc cgggtccgag cacgatgggt ttgcggggag
     3061 gtgcgtcggt cgtcatggaa tcgatcgtaa gcccgaagct accggcgcac cgtgaaaatg
     3121 tgtcgctgtc cctaatcagc tcgcgttctt ggtgttgttc atcttgcgca cggcggtgct
     3181 gtcgtcgtcg atgttgtagt catcgaagtc gctggtgagt tcggccgtgt gtttgtcttc
     3241 gaaacgacgc tcgagacggc gccaggcagg atcgcccagc ttctggccac gcttctttgc
     3301 agcgtccaga tcttttgcaa gacgatcgaa gtcgacttcg ctggtagctt cgaaggcaac
     3361 ctcttcctcg tcctcgtcga actcttctga ttccggtttc tcgttcatga cgcgaaggcc
     3421 tcagatgggc gcgttataga acgagagccc cgcgtcatgc aatggccaaa acaaggattc
     3481 gatcaaggtt cagcgaatca aattgttcat ctcgaagatt ggcatcagca ttgcaaaaac
     3541 gatgcccatg acgaacatgc ccatcacaac aatgagaagt gggcccagca ggcccaccag
     3601 cgcgcccatc atggagtcga gttcgcgctc ctggttggtc gcggcgcgct cgagcatcgt
     3661 gtccagctcg ccgctcgatt ctccggaaga aatgaggtgc acggtcatgg gcggaaagag
     3721 tttgctgtgc gccagcgact tgccgatggg ggcgccttca cgcacccggg cggcggccgc
     3781 ggcgaccgcg tcgcgcatgg gtagattggt gacgacttcc ccggcgatgc gcatggcttc
     3841 gagcaccggc acggcgctgg cggtcaggat gctgaacgtg cgcgtgaagc gggcggtgtt
     3901 gaagccgcgc accagccgtc cgaacagcgg caaccgcagc tgaaaacggt ggaaacggcg
     3961 ccggttgtcc ggattcttga gccagtacac gaagaatatg ctgccgccga tgatgccgag
     4021 cgcgagataa atgccccagt tgcgcaggaa ttcgctgacg gcgatgagca cctgggtggc
     4081 gaacggtagt ttgcccttgc tcgattcgaa cacctcgatg accttcggca ccacgtagac
     4141 catcatgccg gtgacgattc cgaggcagat gatcgacaac acgatgggat agagcatggc
     4201 gccgagcact ttctgccgcg tcacttcgcg gttctcggtg tagtcggcga gccgttcgag
     4261 gatgccgtcg agccggccgg agctttcgcc cgcggcgacg gtggagcgat agatttcggg
     4321 gaagaccttg ggaaattccg ccacgccgtc ggccagcgtg tggccttcca tgacctttcc
     4381 acgcacgccg agcatgatgc tctggacgcg cggcttctcc gattgctggg agaccgcgag
     4441 cagcgcctct tcgagtggca ggccggcctt gaccagcgtg gcgagctggc gcgtgagcag
     4501 cgagaggtcc gatgccgaga caccgcgccg caccgtgaag ctgcgatcgc gtttcgcctc
     4561 gccgtgtttg acctcggcca ccgtgaccgg caacagctgc cgttcgcgca ggag
//
LOCUS       DATDEU010000278        10521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_925417, whole genome shotgun sequence.
ACCESSION   DATDEU010000278 DATDEU010000000
VERSION     DATDEU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925417"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VIV63_06735"
     CDS             <1..795
                     /locus_tag="VIV63_06735"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY6124328.1"
                     /translation="IENSLFQAAANADLSDRVAFDLAEIFQYDIDFVLDIQSGDRFTV
                     VYEEVFQDGVPLRIGNILAAKFVNEGREYRAVRYTDAQGRGQYFSPDGKSLRKAFIRA
                     PVQFSRISSRFNPSRRHPVLNRIRAHRGVDYAAPVGTPVRAAGEGRVRFVGQQGGYGN
                     VIELEHGSGVVTVYGHLSRFAAKLRRGQHVDLAQVIGYVGKTGLATGPHLHYEYRIRG
                     VHKNPQTVPLPDAEPIPGAERDRFLTGTAELVNMLDLPAGPALVAR"
     gene            859..2943
                     /gene="ppk1"
                     /locus_tag="VIV63_06740"
     CDS             859..2943
                     /gene="ppk1"
                     /locus_tag="VIV63_06740"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY6124329.1"
                     /translation="MDQPDLRSVEHYINRELSLLEFNGRVLAQAMDEKVPLLERLRFL
                     CISSNNLDEFFEIRVAGLKQRLELGSDQPGPDGLTITAQLRAIHDRAHGLVEAQYACL
                     TDVVLPALRRQGIELLARATWDAATAQWLERYFEREIEPVLSPLALDPARPFPRIQNK
                     SLNFIVRLAGQDAFGRDTGLAIIQAPRSLPRVVALPEENGHKRFVLLTGIVQHFVSRL
                     FTGMEVLGCYQFRVTRNSDLFVDDEEVDDLRRALEGELAHRRYGAAVRLEVSRDCPPD
                     LDSFLMRQFALNEVDFYEVSGPVNLNRLDALYDLAQRPDLKYPIFTPGLPRRVAGSTD
                     LLAVIRQKDVLLHHPFHSFGPVLDFLRGAAMDPRVLAIKQTLYRTGNESPIVDALVTA
                     AQSGKDVTVIVELRARFDEEANIELSNRLQEAGAHVMYGVVGFKTHCKMSMVVRREAD
                     GLRRYCHLGTGNYHPRTARGYTDYSLFTCDPAIGQDVHELFLQLTSLTQTPRLTKLLQ
                     SPFGLHDAVVQKIDREAQFASFGKPGRILAKMNSLVDPQVIEALYRASRAGAQIDLII
                     RGLCALRPGIPGVSDNIRVRSIVGRFLEHSRVFFFGNDGNSELFLASADWMERNFFRR
                     VEIAFPVREETHRERIVRDLNSYLSDNTQAWTLGRDGRYTRCERGDDMARDAQGSLLA
                     RYAAGSAPVVVP"
     gene            complement(2956..4473)
                     /gene="ppx"
                     /locus_tag="VIV63_06745"
     CDS             complement(2956..4473)
                     /gene="ppx"
                     /locus_tag="VIV63_06745"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY6124330.1"
                     /translation="MARRAQKSVPDVLAAVDLGSNSFHMVVARYTHGQLVILDRLREM
                     VRLGAGLEADGRLNKDVAARALACLERFGQRLRDMRPDSVRVVGTNVFRRARRKEAFV
                     ERAREAIGHPIEIVSGIEEARLIYSGVSHTMAAGSGRRLVCDIGGGSTEIIIGEGLEP
                     LELESLQIGCVRLSEEFFADGRLSQKRMQRARVAARQAIEPYQAAFRRRGFDEAVGSS
                     GTVRAIGDTIRELDPTAVDIGADGLERIIHELSGLEHIRDLKLNAITEDRKLSFAGGV
                     AILAEVFEQLGIESMRVSDGAMREGLLYDLLGRYTDEDARERTVRSMQERYHVDLAQA
                     ERVEATAVDFLSQVEESWPLADPSIEQLVRWATRLHEIGLDVAHSGYHRHGAYLLANA
                     DMAGFPREEQKLLAIMVGGHRRKPAIEEADELTAPWDKRAPAMTLLLRLAVLLHRGRS
                     SVALPAIKLSARADALEMRFPPEWLDDHPLTVADLHQEIETLKSVGFRLRVFTAK"
     gene            complement(4490..4834)
                     /gene="erpA"
                     /locus_tag="VIV63_06750"
     CDS             complement(4490..4834)
                     /gene="erpA"
                     /locus_tag="VIV63_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY6124331.1"
                     /translation="MSEIETETLKFTDAAARKVGDLIRGEGNPNLKLRVFVQGGGCSG
                     MQYGFEFDEQEQDGDTCVENLGVKLLIDPMSVQYLTGAEIDYREGLDGAQFVIRNPNA
                     QTTCGCGSSFSA"
     gene            complement(4845..5312)
                     /locus_tag="VIV63_06755"
     CDS             complement(4845..5312)
                     /locus_tag="VIV63_06755"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6124332.1"
                     /translation="MSIRSERIARERVMFGRRKQTSTRIDTLLGKTATLNGDLEFSGG
                     LHLDGRVNGNVRSNAADGGALSVSESGFIEGNVEVTNIVMNGTVNGDMHASDRLVLGG
                     SARVNGNVHYGVIEMAPGAVITGKLIPLGKGQAVSAESTPEAEPPVAAAKSGA"
     gene            complement(5296..5946)
                     /locus_tag="VIV63_06760"
     CDS             complement(5296..5946)
                     /locus_tag="VIV63_06760"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEY6124333.1"
                     /translation="MLIVAGGVYGMFEFGRYRAGFDVVSGLKQSAELRNEISTQETTI
                     SELRAKVAQLESSTVGQTREREEVQRMIVDLQGQVARANQELAFFRGIVTQNANSAEV
                     KIQQARMVATATANKFRIRVTLVQPMKPDTVVTGVVVLSVDGEVDGKPGRADFATLTG
                     GKRREITFTFRYLENIDEEITMPPGMKPEQLLVEVRSNRRGSAPVQQSYVWSVDPQ"
     gene            complement(6027..6575)
                     /locus_tag="VIV63_06765"
     CDS             complement(6027..6575)
                     /locus_tag="VIV63_06765"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY6124334.1"
                     /translation="MSDWIDSQGYRANVGIVLMRDNGEVFLGQRTGARGWQFPQGGVR
                     QGEPVEDALFRELEEEIGLVREHVTLIASTSDWIRYRLPKRYVRRGRGPTCIGQKQRW
                     FLLRLAVPEDSAAFTFTNTTDPEFDGWRWANYWEPVREVIYFKRPVYVRMLTELAPTA
                     FPGGPPPLPEWWQSEIAATAEE"
     gene            6640..7626
                     /locus_tag="VIV63_06770"
     CDS             6640..7626
                     /locus_tag="VIV63_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY6124335.1"
                     /translation="MTSRPPTTLLLGLLVAIAAIVACLGLVVGSTEIGVRPAIAALFG
                     DIAEPARTVVLELRAPRLLSAFTIGALLALAGVLLQALFRNPLADSYVLGVSGGSSVG
                     ALFALLFGASLWIVQCSAAVGAMAATFLVLALARGGATVRVLLTGVVVASACGALVTL
                     VLVLADNGQLRGMMFWLAGDLGWAAHPWVMLVAALLATAAVTMIARPLDVLATGETTA
                     QSLGLDLRRWRVVIVVLAGLLTAGAVIQGGTIGFIGLVIPHLVRLAFGTAAHRVVVPA
                     AALAGGTLLAAADLVARTAAAPRQLPVGAIMALLGVPMFLVLLRRLEGPRAG"
     gene            complement(7630..8739)
                     /locus_tag="VIV63_06775"
     CDS             complement(7630..8739)
                     /locus_tag="VIV63_06775"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY6124336.1"
                     /translation="MSSRDRHAGPEEELRERLRVLTEEVARNELLLRKTQAREVELLR
                     AHSLGELFDRIVTGLRDAYALDFVTLSLFDPQHELRHLSGNENLTSAVIDEVRFIDAV
                     SPLAPQLVQFDRPWLGRYEARDHELLVSPAMKQGSIALIPLPRDNRAIGVVAFASRDQ
                     NRFTGDLAADFLAHLGVIVAISLENAVNRARLVRSGVTDFLTGWHNRRYFHNRLREEL
                     ARSERTGKTLACLMIDVDNFKEINDRFGHLAGDEALKEVARRAEAEIRAGDTGARFGG
                     DEFAILLAGAEIVHAMKLAERIHHGVGSTPIRVHGGAHVTVTLSVGLATARPQPRSHD
                     YKALAERLIAEADAALYRAKGAGRNRVEMSTHVIG"
     gene            complement(8742..9854)
                     /locus_tag="VIV63_06780"
     CDS             complement(8742..9854)
                     /locus_tag="VIV63_06780"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HEY6124337.1"
                     /translation="MSSLDDLRRRINELDRQILELVGKRQETSREIIRAKRATGHATR
                     DYNREREVLLAAREAGKQYGVSPQVAENILRLLIRTSLTTQEQASVAAQGAGNGRTAL
                     IIGGAGKMGRWFAEFMASQGFVVEIADPTGAPPGFAHLPDWHSSPLNHDFIVIATPLG
                     LTDKILRELAMRRPKGVIFDVGSLKSPLRAGLNALKSHGCRVTSLHPMFGPNTELLSG
                     RHVIFVEAGSPEATAAARELFASTMVEQVVMSLDEHDRLIAYVLGLSHALNIAFFTAL
                     AESGEAAPRLAKLSSTTFDSQLDVASLVAQESPELYFEIQSLNDYGSESLEALSQAVE
                     RLRTAVISRDFESFATLMRRGREYLEDRRTVAERRA"
     gene            9945..>10521
                     /locus_tag="VIV63_06785"
     CDS             9945..>10521
                     /locus_tag="VIV63_06785"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HEY6124338.1"
                     /translation="MELFNDLLPDPLPTEPMALASAWLATALAARQQPNPDAMVLATS
                     TASGQPSARVVLCKEFDVELGRVSFVTNYDSRKGREIAENARAALVFHWDHAHRQVRV
                     EGVVLKAPEPESDEYFASRNWQRRIGAWASAQSQPLRSRDQLTSAVRAAASRFGVPEP
                     TLDNINDPPAAAIPRPPFWGGYYVWADVVELW"
BASE COUNT         1792 a         3476 c         3298 g         1955 t
ORIGIN      
        1 attgagaatt cactgttcca ggcggccgcg aacgccgact tgtccgaccg cgtggctttc
       61 gacctcgccg agatttttca gtacgacatc gacttcgtac tcgacatcca gtccggtgat
      121 cgcttcaccg tcgtatacga agaagtgttc caggacggcg ttccgctgcg catcggcaac
      181 atcctcgccg cgaaattcgt caatgagggg cgcgagtacc gcgccgtgcg ctacaccgac
      241 gcacagggac gcggtcaata cttctcgccg gatggaaaga gccttcgcaa ggcgttcatt
      301 cgcgcgccag tgcagttttc acgaatcagc tcgcgcttca atccgtcacg gcgccacccg
      361 gtgctgaacc gcatccgcgc gcatcgcggc gtcgactacg ccgccccggt gggcacgcct
      421 gtgcgcgctg ccggcgaagg tcgcgtgcgc ttcgtggggc agcaaggcgg ctacggcaac
      481 gtcatcgagc tggagcatgg ctctggcgtc gtcaccgtct acggccatct ttcgcgcttc
      541 gccgcgaagc tgcgccgcgg tcagcatgtc gacctcgcgc aagtcattgg gtatgtgggc
      601 aagacgggcc tcgccaccgg cccacacctg cactacgaat atcgaattcg cggcgttcac
      661 aagaatccgc aaaccgtgcc gcttcctgat gcagaaccca ttccaggtgc ggaacgggat
      721 cgtttcctga ctggcacggc cgagctcgtg aacatgctgg acttgccggc cggccctgcc
      781 ctggtagcgc gctagccaac tgtcacagtt ctccggccgg agctgtgatt tcatagcgcg
      841 aacccaatct accgcgccat ggaccagcca gaccttcgct ccgtcgagca ctacatcaat
      901 cgcgagttat cgctgctcga attcaacggg cgcgtgctcg cgcaagccat ggacgagaaa
      961 gtgccgttgc tcgagcggct ccggttcctg tgtatctcgt ctaacaatct cgacgagttc
     1021 ttcgagatcc gcgttgccgg cctcaaacaa aggctcgagc tcggctcgga ccaacccggc
     1081 cccgatggac tgacaatcac cgcacaactt cgggccatcc acgatcgtgc acatggcctg
     1141 gtcgaggccc aatacgcctg cctcacagac gtggtgctgc cggcgttacg ccgacagggc
     1201 atcgaacttc ttgcgcgcgc gacgtgggac gcggcgacgg cccagtggct cgagcgctat
     1261 ttcgaacgtg agatcgagcc cgtgctttcg ccgctcgccc tcgacccggc ccgcccgttt
     1321 ccgcgcatcc agaacaagag cctgaatttc atcgtccgcc tcgccggtca ggacgcattt
     1381 ggtcgcgaca cagggctcgc catcatccag gcgccgcgct cgctgccgcg tgtggtcgca
     1441 ttgccggaag agaacggaca caaacgtttc gttctgctga ccggcatcgt ccagcatttc
     1501 gtctccaggc tcttcacggg catggaagta ctgggctgtt atcagttccg tgtaacccgc
     1561 aacagcgacc tgttcgtcga tgacgaggaa gtcgacgacc tgcgccgcgc actcgaaggc
     1621 gagcttgccc atcggcgtta tggagcggcc gtgcgcctcg aggtgtcgcg cgattgccca
     1681 ccggatctcg attccttcct catgcggcaa ttcgcgctga acgaagtcga tttttacgaa
     1741 gtctcaggac cggtcaatct gaaccggctc gacgctctct acgatctggc tcagcggccg
     1801 gatctgaaat atccaatctt cacgccgggc ctgcccaggc gcgtcgccgg gtccactgac
     1861 ctgctcgcgg tgatccgtca gaaggacgtg ttgctgcacc atccgttcca cagtttcggc
     1921 ccggtcctgg atttcctgcg cggcgcggcc atggacccgc gcgtactcgc catcaagcag
     1981 acgttgtaca ggacaggcaa cgagtcaccg atcgtcgatg cactggtgac ggcggcccag
     2041 tccggcaagg acgtgacagt gatcgtcgag ttgcgcgcgc gcttcgacga agaagccaac
     2101 atcgaactgt cgaaccgcct ccaggaagca ggcgcccacg tgatgtacgg agtcgtggga
     2161 ttcaagactc actgcaagat gtcgatggtg gtgcgccgcg aagcggatgg cttgcgcaga
     2221 tactgccatc tgggcaccgg caactatcat ccgcgcacgg cacgcggcta taccgactac
     2281 agcctgttca cctgcgaccc cgcgatcggc caggacgtgc acgaactttt cctgcagctc
     2341 accagcctga cgcagacgcc gcggctcacc aaactactgc agtcgccgtt cggacttcat
     2401 gacgccgtgg tgcagaaaat agatcgcgag gcgcagttcg ccagtttcgg gaaaccaggg
     2461 cgcatcctcg ccaagatgaa ttcgctggtg gatccgcagg tcatcgaagc cctgtatcgc
     2521 gcttcgcgcg ccggcgcgca gatcgacctg atcatccgcg gcctgtgtgc ccttcgcccg
     2581 ggaattccag gggtttcaga caatatccgc gtacgttcga ttgtcggacg ttttctcgaa
     2641 cactcgcggg tgttcttctt cggcaacgac ggcaacagcg aactatttct cgccagcgcg
     2701 gactggatgg agcgcaactt cttccggcgc gtggagatcg ccttcccggt gcgcgaggag
     2761 acccatcgcg agcgcatcgt gcgcgacctc aattcctacc tgtccgacaa tacacaagcc
     2821 tggacactcg gacgggatgg gcgctacacg cgctgcgagc gcggtgacga catggcgcgt
     2881 gacgctcaag ggtcgctgct ggcgcgctac gccgccggca gcgcaccggt ggtcgttccc
     2941 tgaagagacg cgacattatt tcgcggtgaa tacccgaagc cggaacccga cgcttttcag
     3001 cgtctcgatt tcctggtgca gatcggcgac ggtgagcggg tgatcgtcga gccattcggg
     3061 cggaaagcgc atttccaacg catcggcgcg agcgctgagc tttatggcgg gaagcgccac
     3121 gctgctgcgc ccgcgatgca ggagcaccgc cagccgcaac agcagcgtca tcgccggagc
     3181 ccgcttgtcc cagggggccg tgagctcgtc agcttcctcg atcgccggct tgcgacggtg
     3241 cccgcccacc atgatggcca gcagtttctg ttcttcacgc ggaaagcccg ccatgtccgc
     3301 attcgccagt aggtaggcgc cgtggcggtg atacccgctg tgcgccacat caagtccgat
     3361 ctcatgaagc cgcgtcgccc agcgtacgag ctgctcgatc gacgggtccg ccagtggcca
     3421 cgattcttcc acctgcgaca ggaaatcgac cgccgtcgct tcgacccgtt ctgcctgcgc
     3481 gagatcgacg tgataacgct cctgcatgct gcgcaccgtt cgctcgcggg catcttcgtc
     3541 ggtgtaccgg cccagaaggt catacagcag gccttcgcgc atcgccccgt ccgacacgcg
     3601 catggactcg atgccgagct gttcgaacac ctcggccagg atcgccacgc caccggcgaa
     3661 tgaaagcttg cgatcttcgg tgatcgcatt gagcttcaga tcacggatgt gctcgaggcc
     3721 actgagctca tggatgatgc gttccagtcc gtcggcgcca atgtcgacgg cggtcggatc
     3781 gagttcgcgg atggtgtcgc cgatggcgcg cacggtaccc gagctcccga cagcttcatc
     3841 gaatccgcgg cggcggaacg ccgcctggta cggctcgatg gcctggcggg ccgcgacacg
     3901 tgcacgctgc atgcgttttt gcgaaaggcg accgtcggcg aaaaactctt cgctcaggcg
     3961 aacacagccg atctgcaggc tctcgagttc gagcggctcc aggccttcgc caatgatgat
     4021 ctcggtgctg ccgccgccaa tgtcgcagac cagccggcgc ccggaaccgg cggccatcgt
     4081 gtgcgagaca ccggaataga tgagtcgcgc ctcttcgata cccgagacga tttcgatggg
     4141 atggccaatg gcttcccgag cgcgctccac gaaggcttcc ttgcggcgcg cgcgccggaa
     4201 cacgttggtt ccgaccacgc gaacgctgtc gggccgcatg tcccgcaagc gctggccaaa
     4261 cctctcgaga caggcgagcg cacgggccgc aacgtccttg ttcaatcgtc catcggcttc
     4321 gagacccgca ccgaggcgca ccatctcccg gagccggtcg agaatgacca gttggccgtg
     4381 cgtgtaacgc gcgaccacca tgtggaagct gttggaaccg agatcgacgg cggcaagtac
     4441 gtcggggacg gacttctgag cgcgtcgcgc cacgtcagtt aggctgacgt caggcggaga
     4501 aggaagaccc gcagccgcag gtcgtttgcg cgtttggatt gcgaatgacg aactgggctc
     4561 cgtcgagccc ctcgcggtag tcgatttccg cacccgtgag gtattggacg ctcatcggat
     4621 cgatgaggag ctttacgccc agattttcga cgcaggtgtc accgtcctgc tcctgttcat
     4681 cgaattcgaa gccgtattgc atccccgagc aaccgccgcc ctgcacgaac acgcgcagct
     4741 tgaggttggg gttgccttcg ccacggatga ggtcgccgac cttgcgggcg gcggcatcgg
     4801 tgaatttcag ggtttcggtc tcgatttcgc tcatggactg cacactacgc gccggatttc
     4861 gctgccgcaa ccgggggttc ggcctccggc gtcgattctg cggataccgc ctggcctttg
     4921 ccgagcggaa tcaacttgcc cgttatgacg gcgcccgggg ccatttcaat gacgccgtag
     4981 tgaacgtttc cgttgacgcg ggcagacccg cccagcacca ggcgatcgct cgcatgcatg
     5041 tcgccgttga cggtgccgtt catcacgata ttcgtgactt cgacgttgcc ttcgatgaag
     5101 ccgctctcgc tgaccgaaag cgcgcctccg tcagccgcat tgctgcgcac attgccgttc
     5161 acacggccgt cgagatgcag gccgccggaa aattcgaggt cgccattgag cgtcgcggtc
     5221 ttgccgagaa gcgtatcgat gcgcgtggat gtctgcttgc gtcgaccgaa cattaccctc
     5281 tcccgagcga tccgctcact gcggatcgac actccacaca tacgactgct gcactggcgc
     5341 ggacccgcgg cgattggatc ggacttcgac cagaagctgt tcgggtttca tgcccggagg
     5401 catggtgatt tcctcgtcaa tattctcgag ataccgaaac gtgaaggtta tctcacgccg
     5461 cttgccgccc gtcagtgtcg cgaaatccgc acgcccgggc ttgccatcga cctcgccatc
     5521 gaccgacagg acaacgacgc cggtcaccac ggtgtccggc ttcatgggct ggaccagggt
     5581 gacgcggatt cgaaacttgt ttgccgtggc cgtggccacc atacgcgcct gctgaatttt
     5641 tacttcggca gagttcgcat tttgggtgac gatcccccgg aaaaaggcca actcctgatt
     5701 cgcacgcgcc acctggccct gcaggtcgac gatcatgcgc tggacctctt cccgctcccg
     5761 ggtctgccct acggtggagg attccagctg cgccactttg gcgcgcaatt cggaaatggt
     5821 ggtttcctgg gtggaaattt cgttgcgcaa ttcggcgctc tgcttgaggc ccgagacgac
     5881 gtcgaatccg gcgcggtaac ggccgaactc gaacatgccg taaacaccgc cggcgacgat
     5941 gagcaatagc agcacgatgg tgatccagcg gcgcgtcggt gcgtaggtcg taacgacgag
     6001 tttgggaggc agtccgggca tcgcgcctac tcttccgcgg tcgccgcgat ttccgattgc
     6061 caccactcgg gcaacggcgg cggaccgccc ggaaacgcgg taggcgcaag ctcggtcagc
     6121 atgcgcacgt atacgggtcg tttgaagtag atcacttcac gcaccggctc ccagtagttt
     6181 gcccatcgcc agccatcgaa ctccgggtcg gtcgtgttcg tgaaggtgaa cgcggcggag
     6241 tcttcgggca ccgcgagtcg cagcaggaac cagcgctgtt tctggccgat gcaggtgggt
     6301 ccgcggccgc gacgcacgta gcgtttcggc agccgatagc ggatccagtc ggaggtcgac
     6361 gcgatcaacg tgacgtgctc gcgcacgagg ccgatctcct cttcgagctc acgaaagagc
     6421 gcgtcctcca cgggctcgcc ctggcgcacg ccgccctgcg gaaattgcca gccgcgcgcg
     6481 ccggtgcgtt gacccaggaa tacttcgccg ttatcgcgca tcagcacgat accgacattg
     6541 gcccggtatc cttgtgaatc gatccaatcg ctcatggcgc atgttttacc gggtgacacg
     6601 cggccgaaca aggtgcgagt ttttgtcaga caatgcagcg tgacttcgcg accccctaca
     6661 accctgctgc tcggcttgct cgttgcaatc gcagccatcg tcgcctgcct cgggctggtc
     6721 gtgggatcca ccgagatcgg cgtgcgtccg gccatcgccg cgctcttcgg tgatatcgcg
     6781 gaacccgccc gaacggtcgt gctggaactg cgcgcaccgc gcttgctgtc cgccttcacg
     6841 atcggcgcgc tgctggcgct cgcgggcgtg ctgctgcagg ccttgttccg caatccactc
     6901 gcggactcgt atgtgctcgg cgtctccggc ggctcgtccg tcggcgcgtt gttcgcgctg
     6961 ttgttcggcg cttcgctgtg gatagtgcaa tgttcggcgg ccgtcggcgc gatggccgcc
     7021 acgtttctcg tgctcgcgct ggcacgtggc ggcgccacgg tgcgcgttct gctcacgggc
     7081 gtcgtggtcg caagcgcgtg cggagcactg gtgaccctgg tcctggtgct cgccgacaac
     7141 ggccagctcc gcggcatgat gttctggctc gccggagatc tcggctgggc ggcacatccg
     7201 tgggtgatgc tcgtggccgc cttgttagcc acggcggcgg tcacgatgat cgcgcgtcca
     7261 ctcgacgtgc tggccaccgg agagaccacc gcgcagagtc tgggactcga cctgcgtcgc
     7321 tggcgggtgg tcatcgtcgt gctcgccggc ctgctcaccg cgggcgcggt gatccagggt
     7381 ggcaccatcg gtttcattgg tctggtgatc ccgcacctgg tacggcttgc tttcgggacg
     7441 gcggcacatc gcgtggtggt accggccgcg gcgctcgccg gcggcacgtt gttagccgcg
     7501 gcggacctgg tggcgcgcac ggcggccgcg ccccgccagc ttccggtggg cgccatcatg
     7561 gcgttgttag gcgtaccgat gttcctggtg ctgttgcggc ggctcgaagg cccacgcgcc
     7621 ggctgaggct cagccgatga catgggtcga catctcgacc cggttgcgcc cggcgccctt
     7681 ggcgcggtac agggcggcgt ccgcttcggc gatgagccgc tccgccagcg ccttgtagtc
     7741 gtgcgaacgc ggctgcggcc gcgccgtagc cagccccacc gacagcgtca cggtcacgtg
     7801 cgcaccgcca tgcactcgga tcggcgtcga accgaccccg tggtgaattc gctcggccag
     7861 cttcatcgca tgcacgatct ccgctccggc caggaggatc gcgaattcgt caccaccgaa
     7921 acgcgcaccg gtatcacccg cacgaatctc cgcctcggcg cgccgcgcga cttccttgag
     7981 agcttcgtcg ccggccaggt ggccgaagcg atcgttgatc tccttgaagt tgtcgacgtc
     8041 gatcatgaga caggccagcg tcttgccggt gcgctccgac cgcgcgagtt cttcgcgcag
     8101 ccgattgtgg aaatagcggc ggttgtgcca gccggtcagg aagtcggtga ctccactgcg
     8161 aaccagccgc gcgcggttca cggcgttttc aaggctgatg gcgacgatga cgccgaggtg
     8221 cgcgagaaaa tccgcggcca gatcgccggt gaaacgattc tgatcgcggc tcgcgaacgc
     8281 cacgacgccg atggcgcggt tgtcgcgcgg cagcggaatc agcgcgatgc tgccctgctt
     8341 cattgccggc gaaaccagca gttcgtggtc ccgcgcctcg taacggccga gccagggccg
     8401 gtcgaactga acgagttggg gcgcgagcgg cgacaccgca tcgatgaagc gcacctcgtc
     8461 gatcaccgcg ctcgtcaggt tctcgttgcc ggagagatgc cgcagctcgt gctggggatc
     8521 gaacagcgaa agcgtcacga agtcgagtgc gtaagcatcc cgcagcccgg tgacgatccg
     8581 atcgaacagc tcacccagcg agtgagcgcg aagcagttcg acttcacgcg cctgcgtctt
     8641 gcgcagcagg agttcgttgc gcgcgacttc ctcggtgagc acccgcaggc gctcgcgcaa
     8701 ttcttcttcc gggcccgcgt gacgatcgcg cgagctcact ctcacgcacg ccgctcggcg
     8761 acggtgcgcc ggtcttcgag atattcgcgg ccgcgccgca tgagcgtggc gaacgactcg
     8821 aaatcccggg agatcaccgc ggtgcgcagc cgctcgaccg cctgtgacag cgcctcgagc
     8881 gactccgagc cgtaatcgtt gaggctctgg atttcgaagt acaactccgg actttcctgc
     8941 gccacgagag acgccacgtc gagctgtgaa tcgaacgtgg tcgacgacaa cttggcgagg
     9001 cgaggcgcgg cttcgccgct ttcggcgagc gccgtgaaga aggcgatgtt cagtgcatgg
     9061 gaaagcccca gcacgtaggc gatgaggcgg tcgtgctcgt cgagcgacat gacgacctgc
     9121 tccaccatcg tcgacgcgaa cagttcgcgc gccgccgcgg tggcttcagg tgagcccgcc
     9181 tcgacgaaga tgacgtgccg gcccgaaaga agctctgtat tcggaccgaa catcggatgc
     9241 agcgaagtca cgcggcagcc atgggatttg agcgcgttga gcccggcgcg caacggtgac
     9301 ttgagcgacc ccacgtcgaa gatgacgccc ttcgggcgac gcatggcgag ctcgcgcagg
     9361 atcttgtcgg tgagaccgag tggcgtcgcg atgacgatga agtcgtggtt gagcggcgac
     9421 gagtgccagt ccggaaggtg cgcgaacccc gggggcgcgc cggttggatc ggcgatctcc
     9481 acgacgaagc cctgtgaggc catgaactcc gcgaaccagc gacccatctt gccggcgccg
     9541 ccgatgatca gcgccgtgcg cccgttgccc gcgccctggg ccgccacact cgcctgctcc
     9601 tgggtagtta gagacgtgcg gatcaggagc cgcaggatgt tctccgcgac ctggggtgac
     9661 acgccgtact gcttgcccgc ctctcgcgcc gccagcaaca cttcgcgctc gcggttgtag
     9721 tcgcgcgtcg catgcccggt ggcacgtttg gcgcgaatga tttcgcggct ggtctcctgg
     9781 cgtttgccga ccagctcgag gatctgacga tcgagttcgt tgatccttcg gcgcaggtcg
     9841 tcgagcgagc tcatgattca ccctgtatta cgtattccga cctagctttt atcggctgcg
     9901 aagcacgact gcaaggctac cgcgtcacgg ataacatatc ggccatggaa ctcttcaacg
     9961 acctgctgcc cgatcccctg cccaccgaac cgatggcgct tgccagtgcc tggctcgcga
    10021 ctgccctggc cgcccggcag caacccaatc ccgacgccat ggtgctggcc acctcgaccg
    10081 cgtcaggcca gccttccgcg cgagtcgtcc tgtgcaagga attcgacgtc gaactgggcc
    10141 gcgtctcctt cgtcaccaac tatgactccc gcaagggccg cgaaattgcc gagaacgccc
    10201 gcgccgcgct ggtgttccac tgggaccacg cgcaccgcca ggtccgggtc gagggagtcg
    10261 tgctgaaagc gcccgaaccc gagagcgacg aatacttcgc ctcgcgcaac tggcagcggc
    10321 gcatcggcgc gtgggccagt gcgcaaagtc agccgctgcg gtcgcgcgac cagctcacct
    10381 ccgccgtacg cgccgccgcg agccgcttcg gcgtgcccga gcccacgctc gacaacatca
    10441 acgatccccc ggcagccgcc attccgcggc cgccgttctg gggtggctac tacgtctggg
    10501 ccgacgtggt cgaactctgg g
//
LOCUS       DATDEU010000279         5707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_634259, whole genome shotgun sequence.
ACCESSION   DATDEU010000279 DATDEU010000000
VERSION     DATDEU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634259"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1617)
                     /gene="gspE"
                     /locus_tag="VIV63_06790"
     CDS             complement(124..1617)
                     /gene="gspE"
                     /locus_tag="VIV63_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY6124339.1"
                     /translation="MNDVAAPPIETPRPQRVGFAFAKRHGVLVNRVHDGIAECVYRPG
                     VAPLAVAEVRRYLGIPIRLEKVEETTFDSLLRQAYEAGNDAMQAAEGVEESTDLAHLA
                     QDLPEQADLLESEDDAPIIRLINAVLTQAVKENASDIHVEPFENRLVVRFRVDGVLRE
                     VLQSRRAVAPLVVSRIKVMSKLDIAEKRLPQDGRISLKIAGRSVDVRVSTLPSGHGER
                     VVMRILDKQAGRLHLSALGMDPVTEQRIADLFRKPHGIILVTGPTGSGKTTTLYAGLE
                     HLNDNTQNIMTVEDPIEYYIDGIGQTQVNTKVEKTFARGLRAILRQDPDVVMVGEIRD
                     LETAQIAVQASLTGHLVLSTLHTNTAVGALTRLRDMGIEPFLLSSSLIGVVAQRLVRT
                     LNPDAKQPFVAGEYERRLLGLGPDDPSPTLYRPGTGPNGFRGRTGIYELVIVDDQMRT
                     MIHDEVAEHELERYARTMTPSIRDDGLERVLRGETTVEEVLRVTRED"
     gene            complement(1614..3701)
                     /gene="gspD"
                     /locus_tag="VIV63_06795"
     CDS             complement(1614..3701)
                     /gene="gspD"
                     /locus_tag="VIV63_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY6124340.1"
                     /translation="MTRKSFRTILLSALLAFVATGLVSAQNNNGQRITPSFKDAEIGQ
                     VIEAVAAATGKTIIPDPRVRAQVTMLSQTPMTPDAFYEAFLALLSVHQFVAVESGGII
                     KIVPDANARQMPNIDLPSRVKSGSDELITQVIAVRNVNAAQLVPVLRPLMPQAAHMAA
                     YPNGNILILSDRASNVARVMRIIERIDQQGDNDVDIINLQHASSSEVVRVVNTFFQQQ
                     SAAEGGGSPSRVIADDRSNSVLVGGEKTLRLRIKALIAHLDTPLESGGDTQVRYLLYA
                     DAEKIATKLKEQISATVAITTGAPPAGGGGAASSVAASADRSTTIWAEPETNALVITA
                     PAKVMRSLMSVVDKLDIRRMQVLVEAIIVDINVNKNAELGVNWAVWSDDDSDAPLGTF
                     QQPVGGVNLATLARSIDDPSNASPFLGTGTTFGIGRIATSGVNFAAKLRAIQGDSNTN
                     VIATPSAVTMDNQEAELKVAQEVPFVTGSYSNTGNTGNNGEVNPFTTVQREEVGTILK
                     ITPQVNGSGALVQLKIELESSSLTGDSGDANSLITNKRTISTNVLIEDGGIVVLGGLI
                     QDSDIRGEQRVPYLGRIPVLGALFKTRSRQTDKTNLMVFIRPKILRDAFATAVETNSK
                     YNYIRDEQRAAGRPKSEILPLLPFSKEPLLPPLPQPLPDATPQPMPPSEVAPPPPAAT
                     SPSSTPENTPPKP"
     gene            complement(3734..4696)
                     /gene="gspC"
                     /locus_tag="VIV63_06800"
     CDS             complement(3734..4696)
                     /gene="gspC"
                     /locus_tag="VIV63_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEY6124341.1"
                     /translation="MEMTANFWNPGASGGGDWRSTLLARAPQVVTFVLALALAAQLAF
                     IVVQMTSRSRQAPPPAIAPLPPPPQLDIGGMVNAHLFGNAAVQASGDAANAPPSSMPL
                     VLAGVLATEDPKEGMAIIGESAQAAKVVAVGQQVPGGAQLHSVYSDRAIIERGGALES
                     VFLPKRTGGAIVGPAPAPMPAASADSNEAAIDRMRKLVNDDPGALSQVLRPQPVFAGG
                     EMKGFRVYPGANRQAFARMGLRAGDMVTAINGTPLNDKDRAQEIFGTLASSTDARVTI
                     TRNGRQQELVLNIAQIAAEAERLGGGGDGNIPVEQAPEPVPGNE"
     gene            4808..5137
                     /gene="clpS"
                     /locus_tag="VIV63_06805"
     CDS             4808..5137
                     /gene="clpS"
                     /locus_tag="VIV63_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY6124342.1"
                     /translation="MPMSETERPDHGGAGLAVEEAEPKTKQPPLYQVVLLNDDYTPME
                     FVVDVLEKFFRMDRMTATRVMLEVHTQGKGICGVFTYEIAETKVALVTSYARDHHHPL
                     MCTLEEA"
     gene            5180..>5707
                     /locus_tag="VIV63_06810"
     CDS             5180..>5707
                     /locus_tag="VIV63_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY6124343.1"
                     /translation="MLSQELEFCLNDAFAKAREARHEFLTVEHLLLSILDTPKVREIL
                     RACGADLAKLKDSLNEHLDRTTPRLPNDEDREVQPTLGFQRVLQRAVFHVQSSGKKEV
                     GVANVLIAIFSEKQSHAVHLLNRAEVTRLDVVNFVSHGLSKIAEEKTAKEEAGSEGAD
                     RDADGGSALDKYTANL"
BASE COUNT          946 a         1756 c         1912 g         1093 t
ORIGIN      
        1 gcaacagctg ccgttcgcgc aggagctgcc gcacgtgttt gacggtgtcg ccttcgacca
       61 caccgttctg ttcgcggccc gcggtatcga gagcggtgta ttcgaaagcg cccacgagag
      121 tgatcagtct tcgcgcgtga cgcgcaggac ttcttcgacc gtggtctcgc cgcgcagcac
      181 gcgctcgagt ccatcgtcgc gaatcgacgg cgtcatcgtg cgcgcgtagc gctcgagttc
      241 gtgttcggcg acttcgtcgt gaatcatggt gcgcatctgg tcgtcgacga tgacgagttc
      301 gtagatgccg gtgcggccgc ggaacccgtt cggccccgtt cccggccggt acagcgtggg
      361 cgagggatcg tcgggtccga gcccgagcag tcgccgttcg tattcacccg ccacgaaggg
      421 ttgcttggcg tccgggttga gcgtgcgcac caggcgctgc gcaacgacgc cgatgaggct
      481 cgacgacagc agaaacggtt cgatacccat gtcgcgcagg cgcgtgagcg cgcccacggc
      541 ggtgttggtg tgcagcgtgg acaacacgag atggccggtg agtgacgcct gtaccgcgat
      601 ctgcgcggtt tcgaggtcgc ggatttcgcc gaccatgacg acgtccgggt cctggcgaag
      661 gatggcgcga aggccgcgcg cgaatgtctt ctcgaccttg gtgttgacct gcgtctggcc
      721 gatgccgtcg atgtagtact cgatcggatc ctcgaccgtc atgatgttct gcgtgttgtc
      781 gttcaggtgc tcgaggccgg cgtacagcgt cgtggtcttg ccggagccgg tagggcccgt
      841 caccaggatg attccgtgcg gcttgcggaa cagatcggcg atgcgttgct cggtgaccgg
      901 gtccatgccg agcgccgaga ggtgcaggcg gcccgcctgc ttgtcgagga tacgcatgac
      961 gacgcgttcg ccatgtccgg acggcaaggt cgagacgcgc acgtcgaccg agcgtccggc
     1021 gatcttgagg ctgatgcgtc cgtcctgcgg aagacgcttc tcggcgatgt cgagcttcga
     1081 catgaccttg atgcgcgaga cgaccagcgg tgccacggca cggcggcttt gcaggacttc
     1141 gcgcagtaca ccgtcgacgc ggaagcgcac caccaaacgg ttttcgaaag gctcgacgtg
     1201 gatgtcggac gcgttttcct tgaccgcctg cgtgagcacg gcattgatca gacgaatgat
     1261 gggcgcatcg tcttcgctct cgagcaggtc ggcctgctcg ggaagatcct gggcgagatg
     1321 cgcgagatcg gtggactctt cgacgccttc ggcggcctgc atcgcgtcgt tgccggcttc
     1381 ataagcctgg cgcagcaatg agtcgaaggt ggtttcctcg actttctcga gcctgatggg
     1441 aatgccgaga taacgacgga cttccgccac cgccagcggt gcgacgcccg gccggtagac
     1501 acattcggcg atgccatcgt gcacgcggtt cacgagcacg ccgtggcgct tggcgaacgc
     1561 gaagccgaca cgctgcggac gaggcgtctc gatcggcggg gcggcgacgt cgttcatggc
     1621 ttgggcggag tattttccgg cgtggacgaa ggcgacgtgg ccgccggcgg cggaggcgcc
     1681 acttccgacg gtggcatcgg ctgcggcgtc gcatcgggca gcggctgcgg caacggcggc
     1741 agcaagggct ccttgctgaa cggcagcagc ggaaggatct ccgacttcgg ccggcccgcg
     1801 gcgcgctgtt cgtcgcgaat gtagttgtat ttcgagttgg tctcgaccgc ggtcgcgaag
     1861 gcatcgcgaa ggatcttcgg acggatgaag accatgagat tggtcttgtc ggtctggcgg
     1921 ctgcgggtct tgaacaacgc tccgagcacc gggatacggc ccaggtacgg cacgcgctgt
     1981 tcgccgcgga tgtcgctgtc ctggatcaaa ccacccagca cgacgatgcc gccgtcttcg
     2041 atgagcacgt tggtgctgat ggtgcgcttg ttggtgatga ggctgttggc gtcgcccgaa
     2101 tctccggtga gcgaggagct ctcgagttcg atcttgagct gcacgagcgc gccgcttccg
     2161 ttcacctgcg gcgtgatctt gagaatggtg ccgacttctt cacgctgcac ggtggtgaac
     2221 ggattcacct cgccgttgtt gccggtgttt cccgtgttgc tgtacgagcc cgtgacgaac
     2281 ggcacttcct gcgcgacctt cagctccgcc tcctggttgt ccatggtgac ggccgaaggc
     2341 gtggcgatga cgttggtgtt ggagtcgccc tggatcgcgc gcagcttggc ggcgaagttc
     2401 acgcccgacg tcgcgatgcg gccgataccg aacgtggtgc cggtgccgag aaacggactc
     2461 gcgttcgaag gatcgtcgat cgaacgcgcg agcgtcgcga ggttcacgcc gccgacgggc
     2521 tgctggaacg tgccgagcgg ggcatcggaa tcatcgtcgg accacaccgc ccagttcacg
     2581 ccgagttcgg cgttcttgtt cacgttgatg tcgacgatga tggcttcgac gagcacctgc
     2641 atgcggcgga tgtcgagctt gtcgaccacg ctcatgagcg agcgcatcac cttggccggc
     2701 gcggtgatga ccagcgcatt ggtttcgggt tcggcccaga tggtcgtgga gcggtcggcg
     2761 ctggcggcca cgctgctggc ggcaccgcca ccgccggccg gaggcgcgcc cgtggtgatg
     2821 gccaccgtcg cgctgatctg ttccttgagt ttggtggcga tcttttccgc atctgcgtac
     2881 agcaggtatc tcacctgcgt gtcgccaccg ctttcgagcg gcgtatcgag atgcgcgatg
     2941 agcgccttga tgcgcaggcg cagggtcttc tcgccgccga cgagcacgct gttcgagcga
     3001 tcgtcggcga tgacgcgcga cggcgagccg ccgccttcgg ccgcggactg ctgctggaag
     3061 aacgtattca ccacgcgcac gacttcgctc gacgaagcgt gctgcaggtt gatgatgtcg
     3121 acgtcgttgt cgccctgctg gtcgatgcgc tcgatgatgc gcatcacgcg ggccacgttg
     3181 ctggcgcgat cggagaggat gaggatgttg ccgttgggat aggcggccat gtgcgcggcc
     3241 tggggcatca gcggacgcaa caccggcacg agctgcgcgg cattcacgtt gcgcacggcg
     3301 atgacctgcg tgatcagctc atcggaaccg gacttcacgc gcgagggcag gtcgatgttc
     3361 ggcatctgcc gcgcgttggc atccggcacg atcttgatga tgccgcccga ttccaccgcg
     3421 acgaactggt gtaccgacaa cagcgcgagg aacgcttcgt agaacgcgtc gggcgtcatg
     3481 ggcgtctgcg acagcatcgt gacctgcgcg cgaacgcgcg ggtcggggat gatggtcttg
     3541 ccggtggcgg ccgcgacggc ttcgatgacc tggccgatct cggcgtcctt gaagctcggg
     3601 gtgatgcgct ggccgttgtt gttctgggca ctgaccagcc cggtcgcgac gaacgcgagc
     3661 agtgccgaga gcaggatcgt ccggaaggat tttcgggtca ttgtcactct gttcattatt
     3721 gttcgttctc gagtcattcg ttcccgggaa ccggctcggg cgcctgctcg accgggatat
     3781 tgccatcgcc gccgcccccg aggcgttccg cctccgcggc gatctgcgcg atgttcaaca
     3841 ccagttcctg ctgccggccg ttgcgggtga tggtgacgcg cgcgtccgtg gaggaggcga
     3901 gcgtgccgaa gatttcctgg gcccggtcct tgtcgttcaa cggggtgccg ttgatcgccg
     3961 tcaccatgtc gccggcgcga aggcccatgc gggcaaaggc ctgacgattg gcgcccgggt
     4021 agacgcggaa acctttcatt tcgccacccg cgaacaccgg ttgcggccgc aacacctggc
     4081 tcagggcgcc cgggtcgtcg ttcacgagtt tgcgcatgcg gtcgatcgcg gcctcattgg
     4141 agtccgctga agccgccggc atgggtgccg gggcggggcc gacgatggct ccgccggttc
     4201 tcttgggaag gaacacggat tccagcgcgc cgccgcgttc gatgatggcg cggtcgctgt
     4261 agaccgaatg cagctgggct cccccaggca cctgctgacc gacggcgacg accttcgccg
     4321 cctgggcgct ttctccgatg atggccatac cttccttggg atcttcagtg gccagtaccc
     4381 ccgcgagcac caggggcatg ctggacggag gcgcgttcgc tgcatcgccg ctcgcctgaa
     4441 cggcggcgtt gccgaataaa tgcgcgttca ccatgccgcc gatatccagc tgcggcggtg
     4501 gcggcagcgg ggcgatcgcc ggcggcggcg cctggcggct ccggctggtc atctgcacca
     4561 cgataaacgc cagttgcgcc gccaaagcca gtgcaagaac gaaagtgacg acctgcggcg
     4621 cacgcgccag cagcgtcgag cgccagtcgc ccccgccgct cgcgcccgga ttccagaagt
     4681 ttgccgtcat ttccatgatg atacgtttcg ctctgcagca gcctcaaggc tttgttttgt
     4741 cttggcaaac cgactacgcc ggtagcgcac ttgtgttgcc attcgacgac ccatataaag
     4801 agcgccaatg cccatgtccg aaaccgaacg ccccgatcac ggcggcgcgg gcctcgccgt
     4861 cgaagaagcc gagcccaaaa ccaagcagcc gcccttatat caggtggtgc tgctcaacga
     4921 cgattacacg ccaatggagt tcgtcgttga cgtgctggag aagttctttc gtatggaccg
     4981 catgactgcg acccgcgtca tgctggaggt acatacgcag ggcaaaggca tctgcggtgt
     5041 gttcacatat gaaatagcgg aaaccaaagt ggcgctggtg acctcttatg ctcgtgacca
     5101 tcatcatcca ttgatgtgta cgctcgaaga ggcctgagtt ccgactctcc ggtgatctaa
     5161 cggtcgagag gttgaaaagg tgttatcgca agagctcgaa ttctgtctca acgatgcctt
     5221 cgccaaggcg cgcgaggccc gccatgagtt cctcacggta gagcatctgc tgctgtccat
     5281 tctcgacacc cccaaggtgc gcgaaatcct gcgtgcctgc ggcgcggatc tcgcaaaact
     5341 caaggactcg ctcaacgaac atctcgaccg caccacgccg cgcctgccga acgacgaaga
     5401 ccgcgaagtg cagccgacgc tcggtttcca gcgcgtcctg cagcgtgccg tgttccacgt
     5461 gcagtcttcg ggcaagaaag aagtgggcgt cgccaacgtg ctcatcgcca tcttcagtga
     5521 aaaacagagt cacgcagttc acctgctgaa tcgcgccgag gtcacgcgcc tcgacgtcgt
     5581 caatttcgtc tcgcacggtc tctcgaaaat cgccgaggaa aaaacggcga aggaagaggc
     5641 gggcagcgag ggtgccgatc gcgacgccga cggcggcagc gcgctcgaca aatacaccgc
     5701 caatctc
//
LOCUS       DATDEU010000280         4757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_195897, whole genome shotgun sequence.
ACCESSION   DATDEU010000280 DATDEU010000000
VERSION     DATDEU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195897"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..941
                     /gene="lpxA"
                     /locus_tag="VIV63_06815"
     CDS             165..941
                     /gene="lpxA"
                     /locus_tag="VIV63_06815"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016976867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY6124344.1"
                     /translation="MSGIDPRAIVSPKAVLAADVTVGPFTVIGDGVTIGAGTWIGPHA
                     VINGPTSIGAGNKIFQFASIGDAPQDLKYKGEPTRLEIGDRNVFREFTTMNRGTVGGG
                     GLTTIGSDNLFMAFTHVAHDCRVGNKCVMANYATLAGHVELGDWVIMGGYAGTHQFTK
                     IGAHAFLGNNSAATRDVPPYVMVVGTPAVPHSINSEGLKRRGFTPEQIRNLKNAYRVL
                     YRSDLKLADAVAELQSRVATQPELKIFVDFIGESTRSLVR"
     gene            956..2110
                     /gene="lpxB"
                     /locus_tag="VIV63_06820"
     CDS             956..2110
                     /gene="lpxB"
                     /locus_tag="VIV63_06820"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY6124345.1"
                     /translation="MRIALVAGETSGDVLGAALIEALRRKFPHAEFAGVAGPKMKAAG
                     CTAWHGIEELAVMGLTEVLTHLPRLMRLRATLTRQILAWRPDVFIGVDYKEFNLGLAK
                     KLKRAGLTTVQYVSPQVWAWRQGRVRTIGASVDLVLCLFPFEPDFYREHGVRAAFVGH
                     PLADQIPLDVDRGAARAELGIDPSARVLAVLPGSRHGEVDKLAGVFAGAAQLLAKRFD
                     GLVTIAPMVTPALRDLFAAHCARNAPDARVRLLDGQARRALAASDVVLVASGTATLET
                     ALSKRPMVVAYKLGAITAFLLRTLGLVKIKHFSQPNLLARKELVPEFFQEAASAENLA
                     NALAHWLEHPAEVAAVQQEFSAIHARLRCDGADRAAAEVADLLATRAMPA"
     gene            2107..2718
                     /gene="rnhB"
                     /locus_tag="VIV63_06825"
     CDS             2107..2718
                     /gene="rnhB"
                     /locus_tag="VIV63_06825"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY6124346.1"
                     /translation="MKLRVRLRVAGVDEAGRGPLAGPVVAAAVILNPARPIRGLADSK
                     VLEPEERESLAILIRERALCYAVAWADAEEIDSINILQATMLAMRRALLGLAVPPQQV
                     FIDGNRCPCADGLGFECLFEAVIKGDAKVPSISAASILAKTTRDALMRDLDNRYPGFS
                     LSGHKGYPTAAHVAALNALGPSPLHRRSFAPVRVREAFVGEEF"
     gene            2946..>4757
                     /gene="dnaE"
                     /locus_tag="VIV63_06830"
     CDS             2946..>4757
                     /gene="dnaE"
                     /locus_tag="VIV63_06830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY6124347.1"
                     /translation="MPSAVFVHLRLHTEFSLQDSVVRIPELVERTVALGMPAVAVTDQ
                     NNLFAMVKFYREGLKHGVKPIIGVDVLLRSGERLPPNRLTLLCKDPGGYQNVANLVTR
                     AWLEGQEKGVPLLSRDWLTRETTAGLIALSGFAEGDVGRAIANGREADALVVAREWKE
                     LFGDRYYLELQRLGRADDEGMVAKTVALSQKAGIPVVATNDVRFLSASDFESHEARVC
                     ISDGAQLADPGRQRKYTEAQFLRSPEEMAALFADIPEALQNSVEIARRCSLPLKLGES
                     RLPNYPLPEGTTVEAHIRAESARGFAEREKVFDASQLERITVYRDRLERELGVICQMG
                     FSGYFLIVADFIRWARANGVPVGPGRGSGAGSLVAYSLGITDIDPLKYDLLFERFLNP
                     ERVSMPDFDIDFCMDGRDRVIDYVSQKYGRERVSQIITYGTMAAKAVVRDVGRVLGMG
                     YGYVDKIAKLIPFELGITLDDALEKEPELKKLYKEDEEIKNLIDLARSLEGLTRNAGM
                     HAGGVVIAPSKLTEFAPLYADDKGGSVVTQFDKDDVEAAGLVKFDFLGLRTLTVIDRA
                     VKIINARRAQNGEAPLDIGALPMDDKATYELMKACKTT"
BASE COUNT          818 a         1558 c         1547 g          834 t
ORIGIN      
        1 gtcgaacccg gcgatacgct caccctcacc gcgaagctcg aacgctccat gcgcggcatc
       61 tggaagttcg ccgtcgtggc atacgtgggg gatgatgagg taacttctgc cgaacttatg
      121 gtcgcgcccg aaaccaacaa gacttcggca aaggcaccgc cggcgtgagc ggcatcgatc
      181 cccgcgccat cgtttctcct aaggctgtgc tggccgcgga tgtcaccgtg gggcctttca
      241 ccgtcatcgg cgacggtgtg accatcggcg cgggcacgtg gataggcccg cacgcggtga
      301 tcaacggccc caccagcatc ggcgccggca acaagatctt ccagttcgcg tccatcggcg
      361 atgcgccgca ggacctcaag tacaaaggcg agcccacgcg cctggaaatc ggcgatcgca
      421 acgtgtttcg cgagttcacg acgatgaatc gcgggacggt gggcggcggc ggcctcacca
      481 ccatcggcag cgacaacctg tttatggctt tcacccatgt cgcccacgat tgccgtgtgg
      541 gcaacaagtg cgtcatggcc aactacgcca ccctcgcggg ccacgtggag ctcggcgact
      601 gggtcatcat gggcggctat gccggcaccc accagttcac caagatcggc gcacatgcgt
      661 tcctcggcaa caattccgcg gcgacgcgcg atgtgccgcc ctacgtcatg gtggtcggca
      721 cgcccgcggt gcctcactcg atcaattcgg aaggcttgaa gcgccgcggc ttcacgcctg
      781 aacagatccg caatctcaag aacgcctacc gcgtcctcta tcgctcggac ctcaagctgg
      841 ccgatgcggt ggccgagctt cagtcgcgcg tggccacgca gccggagctg aagatcttcg
      901 tggacttcat cggcgagtcc acgcgcagcc tcgtgcgtta agaaccgagt tgccattgcg
      961 catcgcactg gtcgcgggcg aaacctccgg tgacgtactg ggcgccgcgc tcatcgaggc
     1021 actgcgccgc aagtttcctc acgctgaatt cgccggagtc gctggcccga aaatgaaggc
     1081 cgcaggctgc acggcctggc acggcatcga agagctggcg gtcatgggac tcaccgaggt
     1141 gctcacgcat ctgccgcgcc tcatgcggtt gcgcgccacg ctcacgcgac agattctcgc
     1201 gtggcggccc gatgtgttca tcggcgtcga ctacaaggaa ttcaacctgg gtctcgcgaa
     1261 gaagctcaag cgcgccggcc tcactaccgt tcaatacgtc agcccgcagg tctgggcatg
     1321 gcggcagggg cgggtgcgca ccatcggcgc gtccgtggat ctcgtgttgt gcctgtttcc
     1381 attcgagccc gatttttatc gcgaacatgg cgttcgtgcc gcctttgtcg gtcatccgct
     1441 cgccgaccag attccactgg acgtcgatcg cggcgcggcg cgcgcggaac tcggcatcga
     1501 tccgtcggcg cgggtgctcg cggtgttgcc cggcagccgt catggcgaag tcgacaagct
     1561 tgccggtgtt ttcgccggcg cggcgcagct gctggcgaag cgattcgacg gactggtcac
     1621 cattgcgccc atggtcacgc cggccctgag agacctgttc gcggcgcatt gcgcccggaa
     1681 tgcgcccgat gcgcgcgtgc ggctgctcga cggccaggcg cgccgtgcgc tggcggcgtc
     1741 cgacgtcgtc ctggtggcgt cgggcacggc gacgctcgag acggcattgt ccaaacgacc
     1801 gatggtcgta gcctacaagt taggggccat caccgcgttc ctgctccgca cgctcggtct
     1861 cgtgaaaatc aaacatttct cgcaaccgaa cctgctggcg cgcaaggaac tcgtgccgga
     1921 gtttttccag gaagcggcga gtgcggagaa tctcgcgaat gcgctggcgc actggctcga
     1981 acatcccgcg gaggtggcgg ccgttcaaca ggagttctcg gccatccatg caaggctgcg
     2041 gtgcgatggc gcggaccgcg cggcggccga agtggccgac ctgctcgcga cgcgggcgat
     2101 gcccgcatga agctgcgtgt acggctgcgg gtggcgggag tcgacgaagc agggcgcgga
     2161 ccgctggcag gtcccgtcgt ggccgccgcg gtcatcctga atccggcgcg gccgattcgc
     2221 gggctggccg attccaaggt cctcgagccc gaggaacgcg agagcctggc catcctcatc
     2281 cgggaacggg cgctgtgtta cgcggttgcc tgggcggacg ccgaagaaat cgacagcatc
     2341 aacatcctgc aggcgaccat gctggcgatg cgccgcgcgt tgttaggcct cgccgtgccg
     2401 ccgcagcagg tgttcatcga tggcaatcgt tgtccgtgcg cggacggtct cggcttcgaa
     2461 tgcctgttcg aggcggtgat caagggcgac gccaaggtgc cgtcgatcag cgcggcgtcc
     2521 atcctcgcca agaccacgcg cgacgcgctg atgcgcgatc tcgacaatcg ctatcccgga
     2581 ttttcgttgt ccgggcacaa gggatatccc acggctgcgc acgtggcggc gttgaacgcg
     2641 cttggcccga gtccgctgca tcgcaggagt ttcgcgccgg tgcgcgttcg ggaagccttc
     2701 gtcggcgaag agttctagcc cttccctggg atttggcggt gacctcgaga acggccggag
     2761 tctcaacgcc gagttgagcg cggccgtctt cctcacggac gagatcgtcg ccggcgcgga
     2821 gtttcgccag aagccggaca acctgggctc gttcggcggc agcacccgtt caagttcccg
     2881 gcccatctcg ttgatccttt tgcctaccgc aataacgcgt gcgttcgtgt gtaaactcgc
     2941 gcgccatgcc atccgctgtt ttcgttcatc tccgcctgca caccgagttt tcgttacagg
     3001 acagcgtggt gcgcatcccg gaactcgtgg agcgcaccgt ggctctcgga atgccggcgg
     3061 tcgcggtcac ggaccagaac aatctgttcg cgatggtcaa gttctatcgc gagggcttga
     3121 agcacggcgt caagcccatc atcggcgtgg acgtgctgct gcgttcaggc gaacgccttc
     3181 cgcccaaccg tctgacgttg ctgtgcaagg atccgggcgg ctaccagaat gtcgcgaatc
     3241 tcgtcacgcg cgcgtggctc gaaggtcagg aaaagggagt gccgctgctc tcgcgcgact
     3301 ggctgacgcg tgagacgacc gcaggattga tcgcattgtc cggtttcgcc gaaggcgacg
     3361 ttggccgggc gattgccaac ggacgcgagg ccgacgcact cgtcgtggcg cgcgaatgga
     3421 aagagctgtt cggcgaccgc tattacctcg agctccagcg tctcggccgc gccgatgacg
     3481 aaggcatggt cgcgaagacg gtggccctga gccagaaggc cggcatcccg gtcgtcgcca
     3541 ccaacgacgt gcgcttcctg tccgccagtg atttcgagtc gcatgaagcg cgcgtctgca
     3601 tctccgacgg cgcccagctc gccgatccgg gccgccagcg caagtacacc gaggcgcagt
     3661 tcctgcgttc gcccgaggaa atggcggcgc tgttcgccga tatccccgag gcgctgcaga
     3721 actcggtgga gatcgcgcgc cgctgttcgc tgccgctcaa actcggcgag tcgcggctgc
     3781 ctaactatcc gctgcccgaa ggaaccaccg tcgaggcgca catccgcgcc gaatccgcgc
     3841 gcggattcgc ggagcgcgaa aaggtgttcg acgcctcgca gctcgagcgc atcacggtgt
     3901 atcgcgatcg cctggaacgc gagctcggcg tcatctgcca gatggggttt tcgggttact
     3961 tcctcatcgt cgcggacttc atccgctggg cgcgcgccaa cggcgtgccc gtgggtccgg
     4021 gacgcggttc gggcgccgga tcgctggtgg cctacagcct cggcatcacc gacatcgatc
     4081 cgctgaaata cgacctgctg ttcgagcgct tcctgaatcc ggaacgcgtg tcgatgcccg
     4141 acttcgacat cgacttctgc atggacggac gcgaccgcgt catcgactac gtctcgcaga
     4201 agtacggccg cgagcgcgtc tcgcagatca tcacctacgg caccatggcg gcgaaggccg
     4261 tggtgcgcga cgtaggccgc gtgctcggca tgggttacgg ctacgtcgac aagatcgcca
     4321 agctcatccc gttcgagctc ggcatcacgc tcgacgatgc gctcgagaaa gaacccgaac
     4381 tcaagaagct ctacaaggaa gacgaggaga tcaagaatct catcgacctt gcccgctcgc
     4441 tcgagggcct gacgcgcaac gccggcatgc acgccggcgg cgtggtcatc gcgccgtcga
     4501 agctcacgga attcgcaccg ctgtacgccg acgacaaggg cggcagcgtc gtcacgcagt
     4561 tcgacaagga cgacgtcgag gccgcgggcc tcgtgaagtt cgacttcctg gggctgcgca
     4621 cgctcacggt catcgaccgc gccgtgaaga tcatcaacgc gcgccgtgcg cagaacggcg
     4681 aagcgccgct ggacatcggc gcgctgccga tggatgacaa ggccacctac gagctgatga
     4741 aggcctgcaa gacgacc
//
LOCUS       DATDEU010000281         2937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_341515, whole genome shotgun sequence.
ACCESSION   DATDEU010000281 DATDEU010000000
VERSION     DATDEU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341515"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..661)
                     /gene="msrB"
                     /locus_tag="VIV63_06835"
     CDS             complement(98..661)
                     /gene="msrB"
                     /locus_tag="VIV63_06835"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY6124348.1"
                     /translation="MDEANNSRRGFLLGALLSPVALAVSACARGAEPPKPANVTIESF
                     SAAGKSQGKATVPRLVKADEEWRRQLSADSYNVTRRSGTERPFSGKYNTNHDDGLYRC
                     ICCDTALFDSRTKFESGTGWPSFWKPISSANVLEHGYSAISQGTEVNCKLCDAHLGHV
                     FDDGPEPTGLRYCMNSVALNFVERAAG"
     gene            complement(676..2445)
                     /gene="msrA"
                     /locus_tag="VIV63_06840"
     CDS             complement(676..2445)
                     /gene="msrA"
                     /locus_tag="VIV63_06840"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY6124349.1"
                     /translation="MLRTLSVGLALVASVLSVNACGSEAAVKIPAPAFDAPAAPGKLQ
                     TAVVAGGCFWGVQGVYQHLKGVKSVLSGYAGGDGSRADYESVSAGDSGHAESVQIVYD
                     PAQVTYGQILQVFFSVAHDPTQLNRQGPDTGTQYRSAIFYTSDTQKKIAQDYIAQLDK
                     GRLFPRQIVTRVDPLKQFYEAESYHQDYLLNHPSNPYIVINDLPKVRNFQSTLPSLYV
                     DKPVTVRASKRDAKAAPAAPVGGGAMMMAAAEKPAEGGAMMSAQGASGPSKTEGPLPP
                     LTGATGWLNSKPLTPEGLRGKVVVVDFWAYSCINGLRAMPYVNAWYRHYKDSGLVVLG
                     VHSPEFAFEKDIENVRAAVKKFDIQYPVALDSGLEIWKAFNNRFWPAHYFVDAKGQIR
                     GHHFGEGKYAKSEREIRRLLVEAGAKNLPDPLDEAAGEGVAKPADMANVASPETYVGY
                     ARAENFVSPGAFARDAKKSYAIPKALTLNQWALAGPWQVAREHARLDGAPGRIAFRFK
                     ARDLHLVLGPASSGKPVRFRVTLGGAPPKTDAGMDVDANGNGVVREHRLYQLIREKGP
                     VAEQEFVIEFLDAGVDAYSFTFG"
     gene            complement(2665..>2937)
                     /locus_tag="VIV63_06845"
     CDS             complement(2665..>2937)
                     /locus_tag="VIV63_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HEY6124350.1"
                     /translation="GAGIGERPRATPATGIDAYVWVKPPGESDGSSTAIPNDEGKGFD
                     RMCDPTYGGNPRNNNNSTGALPNAPLSGHWFQAQFQQLLQNAYPPL"
BASE COUNT          523 a          934 c          918 g          562 t
ORIGIN      
        1 gaatcgaaat aactcggcca tcggaaattc accttctggt ttgtcgtact aaccagagcg
       61 gaatttccac ctcgcgggga tcatcccggc gggaagatca gccggcggcg cgttccacga
      121 agttgagcgc gaccgagttc atgcaataac gcaggccggt cggctcggga ccgtcatcga
      181 acacgtgccc gaggtgcgcg tcgcacagtt tgcagttgac ctcggtgcct tgtgagatgg
      241 cgctgtaacc gtgctcgaga acattggcgc tcgaaatcgg cttccagaag ctgggccagc
      301 cggtgccgga ctcgaacttc gtgcgcgaat cgaacaacgc cgtgtcgcag cagatgcagc
      361 gatacagccc gtcgtcgtgg ttcgtgttgt atttgccgct gaacggacgc tcggtgcccg
      421 agcggcgcgt cacgttgtat gaatcagcgg agagttgccg gcgccattcc tcgtcggcct
      481 tgaccagccg cggcacggtg gccttgccct ggctcttgcc cgccgctgaa aaactctcga
      541 tcgtcacatt ggcgggtttc ggtggttcgg cgccgcgtgc gcaggctgaa accgccaacg
      601 ccaccggcga cagcaacgcg cctaacagga atccgcgccg tgaattgttt gcctcgtcca
      661 tcaagtcctc cgcgctcagc cgaaagtgaa tgaataggcg tcgacgcccg cgtcgaggaa
      721 ttcgatgacg aactcctgtt cggccaccgg ccctttctcg cgaatgagct ggtacagacg
      781 atgctcgcgc accacgccat tgccattggc gtccacgtcc atacccgcat cggtcttcgg
      841 cggcgcgcca ccgagagtca cgcggaaccg cacgggcttg cccgacgacg cgggacccag
      901 cacgagatgc agatcacgcg ccttgaagcg gaacgcgatg cgacccggtg cgccgtcgag
      961 ccgtgcgtgt tcacgcgcga cctgccacgg accggccagt gcccactggt tgagcgtgag
     1021 cgccttggga attgcgtagc tcttctttgc gtcgcgcgcg aaggcacccg gcgagacgaa
     1081 attttccgcg cgagcgtaac ccacgtacgt ttccggtgac gcgacgttcg ccatgtctgc
     1141 aggcttggcg acaccctcgc ccgcggcttc gtcgagtgga tcgggcagat tcttcgcgcc
     1201 cgcttcgacg agcagcctgc ggatttcacg ctccgacttc gcgtatttgc cttcaccgaa
     1261 gtgatggcca cggatctgac ccttggcgtc gacgaaatag tgcgcgggcc agaaccggtt
     1321 gttgaacgct ttccagatct cgaggccgct gtcgagtgcg acgggatact ggatgtcgaa
     1381 cttcttgacg gccgcgcgca cgttctcgat gtccttctcg aacgcaaact cgggcgaatg
     1441 cacgccgagc acgacgagac cggaatcctt gtaatggcga taccacgcgt tcacgtacgg
     1501 catcgcgcgc aggccgttga tgcacgagta cgcccagaaa tcgacgacca cgaccttgcc
     1561 gcgcaggcct tcgggcgtga gcggcttgct gttgagccag cccgtggcgc cggtgagcgg
     1621 cggcaatggc ccttcggttt tcgaaggccc gctggcgccc tgcgccgaca tcatggcgcc
     1681 gccttcagcc ggtttttcag cggccgccat catcatggcg ccgcctccca ccggcgcggc
     1741 gggcgccgct ttcgcgtcgc gcttcgacgc acgcaccgtc accggtttgt cgacgtacag
     1801 cgaaggcagc gtgctctgga agttgcgcac cttcggaagg tcgttgatga cgatgtaggg
     1861 attggacgga tgattgagca ggtagtcctg gtgataactt tctgcttcgt agaactgctt
     1921 cagcggatcg acgcgcgtca cgatctggcg cgggaacagg cggcctttgt cgagctgcgc
     1981 gatgtaatcc tgcgcgatct tcttctgcgt gtcgctcgta taaaagatgg cggagcgata
     2041 ctgcgtgccc gtgtcggggc cctggcggtt caattgcgtg ggatcgtgcg ccacggagaa
     2101 gaacacctgc aggatctggc catacgtcac ctgcgcgggg tcgtagacga tctgcaccga
     2161 ctcggcgtgg cccgaatccc cggcgctcac cgattcatag tcggcgcggg agccgtcacc
     2221 cccggcatac ccggacagca cgcttttgac acctttcagg tgttgataaa ccccctgcac
     2281 tccccagaag caaccgcctg cgacgacggc cgtttgcagc ttgcccgggg ccgccggagc
     2341 atcgaaggcc ggcgccggta ttttcaccgc cgcttcggag ccgcaggcgt tgacgctcaa
     2401 tacgctcgcc acgagcgcca ggcccactga aagtgttcgc aacatgatga ttcctcgcag
     2461 gtcgatacgc aggagaccgg cgcttcggat gcattatttc gccacggccc cgtttctcaa
     2521 ggggtggcgg attgtggcat gcggacgagg aatcgaaagt cgagtgacag acgcaggcac
     2581 gtcctgtgcc tacgtctgcc actcgccaag acgtgtggtt cagcagggag ggtcgctgaa
     2641 ccgcacgtct taaaaccgcc tcacctagag cggtggatag gcgttctgca acaactgctg
     2701 gaactgcgcc tggaaccagt gtcccgagag cggcgcattc ggcaacgcac cggtcgagtt
     2761 gttgttgttg cgcgggttac cgccataggt cggatcgcac atgcgatcga aacccttgcc
     2821 ctcgtcgttc ggaatggccg tgctcgagcc atccgattca cccggcggct tcacccagac
     2881 gtaggcatcg atacccgtgg cgggcgtcgc acgcggacgc tcgccgatgc cggcacc
//
LOCUS       DATDEU010000282         2914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_341545, whole genome shotgun sequence.
ACCESSION   DATDEU010000282 DATDEU010000000
VERSION     DATDEU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341545"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VIV63_06850"
     CDS             complement(<1..640)
                     /locus_tag="VIV63_06850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6124351.1"
                     /translation="MTAGVPVTPTLSICIPTYNRAANLRNLFRSLTEVKTLFREQIEI
                     CISNNASPDDTAAVIAEHEAALDLKVAHQTENIGATRNIIAVTKMMSGNWGIWVGDDD
                     EVLPAAIAELLKYLGTIDRNGWVLVETQNLEGGDQYLKQFREGEYQSPEYRRALLKHG
                     IYSLSFMGVHVFPRSAVPAFSVLTVEEGQPWPQIAVLMRLLIEAGQKMYILKK"
     gene            complement(637..1728)
                     /locus_tag="VIV63_06855"
     CDS             complement(637..1728)
                     /locus_tag="VIV63_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6124352.1"
                     /translation="MNVPAGTQLFSDDAKTAQSLKTAISIVECPSCSLVQLSGEPVAY
                     ENVSSSSSFVSGQLTEHRLEQLRELFKMKGAKAGTGRLLEIGCGDGHLLENSKGLFEK
                     SVGVEPTKKNAESAKSRGLDVHQMFMSKDATVAGGPFDYFCSFHVLEHVTDICSVLQG
                     ISKALTDDAVGIIEVPSTEAAIENKRFGDFMPDHLNYFVEGTLRMALEWNGFVVERIY
                     RDWGREHLVAYVRKRSKVSSLNYIVDRQAALKDLVAKVNARKLPFAVWGVSHHLLPYI
                     PTLSGIGSFIAVDGSSSKIGKFIPSTDISVRPKAALLDLPHCYVALTAPRFKDEIMGE
                     LSTIYKSTVEDTQLSTWLGFSVYECKSAA"
     gene            2028..>2914
                     /locus_tag="VIV63_06860"
     CDS             2028..>2914
                     /locus_tag="VIV63_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124353.1"
                     /translation="MIVKVGGWLFIVNALLALWSLMLPMPFGGGASIAASLFTAALFG
                     SVGVGLLNQKNWARWLALGMSLLTWTLGTAAVLYALPGTLRLVFAASGMFSFLIVIFV
                     FVAAIVALVIIINFKLFHYLNSDEGRFEFDTPDTETQAVLKSAGVYALVLFAFFMADY
                     RSSKASVDSAALERALRDSRDRDAEERRHEAARRDAVARAEDERARVEQALQEQEPVQ
                     EFAMEPSADSSQYAPAPDAPSPPDASYGSAAPVEAEPRESEQEPQTNKILKCRDSSGA
                     VSFTQGYCPPGTQRVETPNA"
BASE COUNT          547 a          868 c          922 g          577 t
ORIGIN      
        1 ctttcttcag gatgtacatc ttctggccgg cttcgatgag cagccgcatc agcaccgcga
       61 tctgcggcca gggctgacct tcctcgaccg tgagaacgct gaaggccggc accgccgatc
      121 gcgggaacac atgaactccc atgaagctca gcgagtagat gccgtgcttc agcaatgccc
      181 ggcgatattc cgggctctga tattcgcctt cgcgaaattg cttgaggtat tggtctccac
      241 cctcgaggtt ctgggtttcc accagcaccc agccattgcg gtcgatcgtg cccaggtact
      301 tcagcaactc ggcgatggca gcgggaagca cttcgtcgtc gtcaccgacc cagattcccc
      361 agttacccga catcatcttc gtgaccgcga tgatgttgcg cgtcgcgccg atgttctcgg
      421 tctggtgcgc cactttcaga tcgagcgccg cctcgtgctc ggcgatgacc gccgcggtgt
      481 catcgggcga tgcgttgttc gagatgcaga tctcgatctg ctcgcggaac agcgtcttca
      541 cttcggtcag ggaccggaag agatttcgaa ggttggctgc ccggttgtag gtaggtatgc
      601 agatcgacag ggtaggcgtg acaggaactc cggcggtcat gcggcggatt tgcactcgta
      661 gacgctgaag ccgagccacg tcgaaagctg cgtgtcctcg acggtcgatt tatagatggt
      721 cgacagctcg cccatgatct cgtccttgaa ccgcggcgcg gtcagggcga catagcagtg
      781 cggaaggtcg agcagtgccg ctttaggccg gaccgagatg tccgtggacg gaatgaactt
      841 gccgatcttc gacgaagagc cgtcgacggc gatgaagctg ccgatgcctg acaaggtcgg
      901 gatgtaagga agcaggtgat ggctcacgcc ccagacggcg aacggtagct tgcgcgcatt
      961 caccttggcg acgagatcct tgagcgccgc ctgccggtcg acgatgtagt tgaggctcga
     1021 aaccttcgaa cgcttgcgca cgtatgcgac caggtgttcg cggccccagt cacgatagat
     1081 gcgttcaacg acgaagccgt tccactcgag cgccatgcgc agcgtgcctt cgacgaagta
     1141 gttcaggtgg tcgggcatga aatcgccaaa ccgcttgttc tcgatcgcgg cctcggtcga
     1201 cgggacttcg atgatgccga cggcgtcgtc ggtcagagcc ttggaaattc cctgcagcac
     1261 cgagcagatg tcggtcacgt gttcgagcac gtggaacgag cagaagtagt cgaacgggcc
     1321 gccggcaacc gtcgcgtcct tcgacatgaa catctggtgg acgtcgaggc cgcgcgactt
     1381 ggcgctctca gcattcttct tcgtgggctc gacgcccacg gatttctcga acaggccctt
     1441 cgaattctcc agcaggtgcc cgtcgccgca gccgatttcc agcagccggc cggttccggc
     1501 cttggcgccc ttcatcttga acagctcccg caactgctcg agccgatgct ccgtgagctg
     1561 gcccgaaacg aacgacgacg acgacgaaac gttttcgtag gcgacgggtt cgccggatag
     1621 ctgtaccagc gagcaggagg ggcattcgac gatggaaatg gctgtcttga gcgactgcgc
     1681 ggtctttgcg tcatcggaaa agagctgggt tcccgccgga acgttcaaaa cgatggattt
     1741 gcggtcatcg gcggggaaat tacaaagtct gcagtgcatc acagcgagaa gcctccacaa
     1801 ggaatggccg gcattttagc tcgaaaaccg ggctccgggc gggaacaagc acccagggtt
     1861 agccccaaac cgccgccccg gcggtgccgc ccgtgcctgg cgcgatcggc cgggcggcgc
     1921 cggcagtcga acttcagcca gggcaaaggg tgtgaccgac ttcacgggat ctagttagtt
     1981 cgcacatccg gccgccatta ccatcggtgg cacccgggag gtggttcttg atcgtcaaag
     2041 tcggtggctg gctcttcatc gtcaacgcgt tgctcgcgtt gtggtcgctc atgctgccaa
     2101 tgccgtttgg cggcggtgca tccattgccg cgtcgctgtt cacggccgca cttttcggca
     2161 gtgtgggcgt gggattgctg aaccagaaga actgggcgcg ctggctcgcg ctcggcatgt
     2221 cactgctgac gtggacgctc ggaacggccg cggtgctcta tgcgctcccc ggtacgctgc
     2281 gcctcgtgtt cgcggcgagc ggcatgttct cgttcctgat cgtgatattc gtgttcgtgg
     2341 cagcgatcgt cgcgttggtg atcatcatca acttcaagtt gttccactac ctgaattccg
     2401 atgaaggcag gttcgagttc gacacaccgg ataccgagac gcaggccgtg ctcaagtctg
     2461 ccggtgtcta cgccctcgtg ttgttcgcat tcttcatggc cgactatcgg tcgtcgaagg
     2521 catcggtgga ttcggcggcg ctggagagag cgctgaggga ttcgcgggat cgtgatgcgg
     2581 aagaacggcg acacgaggca gctcgaagag acgcggtggc gcgtgccgaa gacgaacgcg
     2641 cgcgggtcga gcaggcgctg caggaacagg agccggtgca ggagttcgcg atggaaccgt
     2701 cagcggatag ttcgcaatac gcacctgccc ccgatgcgcc gagtccgcca gacgcgtcct
     2761 atggatctgc agcccccgtc gaggcagaac ctcgcgagag cgaacaagag ccccagacaa
     2821 acaagattct caagtgccgt gattcttccg gcgccgtctc attcacgcag ggttactgcc
     2881 cgcccggaac ccagcgggtc gaaactccca acgc
//
LOCUS       DATDEU010000283         6536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_341571, whole genome shotgun sequence.
ACCESSION   DATDEU010000283 DATDEU010000000
VERSION     DATDEU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341571"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="efp"
                     /locus_tag="VIV63_06865"
     CDS             complement(<1..527)
                     /gene="efp"
                     /locus_tag="VIV63_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY6124354.1"
                     /translation="MSSYTTNEIRGGMKLLIDGDPYAVIDNEYVKPGKGQAFNRIRVR
                     NLKTGRTVEKSYRSGESIEAADVMDQEMQYLYKDGDFWAFMNPENYEQLTAGEAAVGD
                     SAKWLKDGIVCIVTLWNGVPLVVTPPPHIDLKVVETDPGLKGDTATGGQKVAKLETGA
                     SVRVPLFINEGELLR"
     gene            727..2793
                     /locus_tag="VIV63_06870"
     CDS             727..2793
                     /locus_tag="VIV63_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6124355.1"
                     /translation="MTDNSAVPLIVFSPQREPSEFINSLMRKAGEPVHCTWIPAVRDL
                     GEALEQLNPELLICAYVSETDAKQAATVRDQLAPQVPMILVRPQISEGDIAVGMRMGA
                     RDVVSLSATDRVQAVVSRELRSFRLERALNTTLQSASDYRRQLQSVLQRSNDAILQVQ
                     EGIVVDANQTWLELFGYADATAVVGQPLMDLFEDRSHAALKGALVACAQGKWTDHPLK
                     VTALLGDSTQLQLDLVLTPGEFENEPCVRIMVPARKRDDRQLTTDLSDAVKRDATTGL
                     LLRRPLLDAVGVRIGAPPAGGVRFMALVRPDKFAELEKDIGARASEQLVGEYATTLKS
                     HLGPNDIAGRFSGVSFLALLERGNTHDVEAWAEQVLARFSENTSTVGQKSLHSSCTIG
                     LTTVPTAGSNIDAIVADVVEAVRRGRSQGGNQIFHLDKADTDTRVQAYDKIWVKHIKS
                     ALMENRFRLVQQPVASLQGDDPNMFDVLVRMLDHQGKEVLPAEFMAAAERNDLLKNID
                     RWVVGASLSFAAKMKPGALFVRLSRDSVLDAGFTDWLKTQLANGTHHPERICFQITES
                     IFEQYMNAAVTQLGALEKMGFRLALERFGSGRDPVKLLTAVPLDFIKIDGALVQDLAG
                     NFETQTMVKKLVEHAGKLKIETIAERVEDANTMAVLWQLGVQFIQGYFVNAPEEVVIT
                     DGQGRR"
     gene            2801..3694
                     /locus_tag="VIV63_06875"
     CDS             2801..3694
                     /locus_tag="VIV63_06875"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124356.1"
                     /translation="MSKFLLLAMTMLPLAATAAAPPAVERNIVYGMYSGLALLLDVHR
                     PAKPNGFGVIFISGSGWQSGLEYGAAPLKEQQVPLWGPPLLSAGYTVFAINHRASPRF
                     HYPAAVDDVQRAIRFVRFHARDYGIDGTRLGGVAGSSGAHLLGLAAMLGAAGNPQDTD
                     PVNRESATLAAVVLRAPPLDLRTIDTPEGTAYIVSFMEAPKSNASAAQSYAAASPITH
                     VGARSPPVLLLHGDADTVIPVDQSVAMKAALEAARVPVKLVTIPGGIHGADFGAAQKR
                     ADWPDYFGETIAWLDRYLRGK"
     gene            complement(3703..4722)
                     /locus_tag="VIV63_06880"
     CDS             complement(3703..4722)
                     /locus_tag="VIV63_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY6124357.1"
                     /translation="MAPRTRVLFLLVCSMTASVTPAQTAERQAAPRWALAIHGGAGVI
                     ERGDLTPEKEVAYRAAMLNAMGAGARVLKRGGSSLDAVEAVIRLLEDDPLFNAGRGAV
                     FTAEGRNELDASIMDGSTRKAGAVAGVTRTRNPISLARAVMEKSRHVMLAREGADQFS
                     VEQGLEQVDPGYFRTEQRWQQLLDWRRDNAQLLDPTHSRGTVGAVAIDVNGHVAAGTS
                     TGGMTGKRWGRIGDSPVVGAGTYAADGNCAVSATGSGEFFIRTSAARQLCDRIAWRGD
                     SVQVAASATIADIGSIGGDGGLIALDGQGNIAFAMNSSGMYRGWVTSAVGAATAIYSN
                     EAGPK"
     gene            4825..5439
                     /locus_tag="VIV63_06885"
     CDS             4825..5439
                     /locus_tag="VIV63_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY6124358.1"
                     /translation="MTIYGLLTFCAVYALAVAAPGPGIAAIVARGLAHGTKGAPAFIG
                     GFIVGDLLWFAIAATGLAALARTAAALFVAIKWAGVAYLLYLAYKLWTAPAKRVEVDA
                     ADRKQHGWNAFLASLMLTLANPKAILFFLALLPTVIDLESMNTVRFLEISAAIIVVQP
                     LVLGSDALLAARARELFTTPKAVQRLNRSSGIAMASAAVVVATR"
     gene            complement(5442..>6536)
                     /locus_tag="VIV63_06890"
     CDS             complement(5442..>6536)
                     /locus_tag="VIV63_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY6124359.1"
                     /translation="GLLAAYLNLDEVIRIIRREDEPKPVLMKRFKLTEIQAEAILETK
                     LRHLAKLEEMKIRDEQKKLAEEREELEGILKSKARLKKLVASEIEADAEKYGDDRRTK
                     IVEREAAQAIDESELVANEPATIVLSKGGFVRSAKGHEIDARSLTYKAGDEYQHAARG
                     RSVQQAVFLDSTGRAYSLVAHSLPSARGLGEPLSSRVDPPDGAKWRGVMIGEPEDLWL
                     VASDAGYGFTVRLKEAYASKKAGKTLLNIPDGALVLPPAPVPGDDALVAVTSEGGKLL
                     VFPVKDVPELARGKGNKLFDIPSKKFESREDFLTGMTVVPKDKSLVVRSGDRKMTIDW
                     KDLKDYRGQRAQRGSVLPRGWRTVDLLETE"
BASE COUNT         1080 a         2262 c         2047 g         1147 t
ORIGIN      
        1 atgcgcagca attcaccttc gttgatgaac aggggcactc gcaccgaggc gccggtttcg
       61 agcttggcga ccttctggcc accggtggcg gtgtcgccct tgaggcccgg gtcggtctcg
      121 acgaccttga ggtcgatgtg cggcggcggg gtcacgacca gcggcacgcc attccacaac
      181 gtgacgatgc agacgatgcc gtccttgagc cacttcgccg agtcgccaac cgcggcttcg
      241 ccggcggtga gctgctcgta gttctcggga ttcatgaagg cccagaagtc gccgtccttg
      301 tagaggtact gcatctcctg gtccatgacg tcggcggctt cgatcgattc gccggagcga
      361 taactcttct cgaccgtgcg gcccgtcttc agattgcgaa cgcgaatgcg attgaacgcc
      421 tggcccttgc ccggtttcac gtactcgttg tcgatcacgg cgtacggatc gccgtcgatc
      481 agtagcttca ttccgccgcg gatttcattg gtcgtgtaac tggacatggg gattctcgta
      541 gttgcccgaa attgggcctg caaaaaagac cgcgcatgat actcaccccg ggaaatcccc
      601 gccatacgaa acagtcgcga tcaactgtga gccggcgctc agccagtacc ccgggggacg
      661 ggtgctaaac tcgccccgct tgtttgaacc gccttgcacg ctgggcgcac cttccgggga
      721 ctcaaattga cggacaattc cgccgttccg ctcatcgttt tctcaccgca gcgtgagcct
      781 tccgaattca tcaacagcct gatgcgcaag gccggcgagc ccgtgcactg cacctggatc
      841 ccggcggttc gcgacctcgg cgaggcgctg gaacagctca accccgaact cctgatctgc
      901 gcgtacgtgt ccgaaaccga cgccaagcag gccgcgaccg tgcgcgacca gctcgcgcca
      961 caggtgccga tgatcctcgt gcgtccgcag atttccgaag gcgacatcgc ggtcggcatg
     1021 cgcatgggtg cgcgcgacgt ggtgtccctg tcagcgaccg atcgcgtgca ggccgtcgtg
     1081 tcgcgcgagc tgcgctcgtt ccggctcgag cgcgccctga acacgacgtt gcagtcggcg
     1141 agcgactatc ggcgccagtt gcagtcggtt ctgcagcgct ccaacgacgc catcctgcag
     1201 gtgcaggaag gcatcgtcgt cgatgccaac cagacctggc tcgaactgtt cggttacgcg
     1261 gacgccaccg cggtcgtcgg ccagccgctc atggacctgt tcgaggatcg ctcgcatgcc
     1321 gcgctcaagg gtgcgctcgt ggcctgcgcc cagggcaaat ggaccgacca tccgctgaaa
     1381 gtcacggcgt tgctcggcga cagcacgcag ctgcagctcg acctggtgct gacgcccgga
     1441 gaattcgaga acgagccctg cgttcgcatc atggtgcccg cgcgcaagcg cgacgatcgc
     1501 cagctcacga cggacctgag cgatgcggtg aaacgcgatg cgaccaccgg cctgctgcta
     1561 cgccggccgc tgctcgatgc cgtgggcgtg cgcatcggcg cgccacccgc gggcggcgtg
     1621 cgattcatgg cgctggtgcg cccggacaaa tttgccgagc tcgaaaagga cataggcgcg
     1681 cgcgccagcg aacaactcgt cggtgagtac gcgaccacgc tcaaatccca cctcgggccc
     1741 aacgacatcg cgggccgttt ctccggcgtg tcgttcctcg cgctgctcga acgcggcaat
     1801 acccacgacg tcgaggcctg ggccgaacag gtcctggcgc gcttcagcga aaacacctcg
     1861 accgtcgggc agaaatccct gcactcgagc tgcaccatcg gtctcaccac cgtacccacg
     1921 gccggctcca acatcgatgc catcgtcgcc gatgtcgtcg aagccgtgcg tcgtggacgc
     1981 agccagggcg gcaaccagat tttccacctc gataaggccg acaccgacac gcgcgtgcag
     2041 gcgtacgaca agatctgggt gaaacacatc aagtcggcgc tcatggagaa ccgcttccgc
     2101 ctcgtgcagc agccggtggc cagcctgcag ggcgacgacc cgaacatgtt cgacgtgctg
     2161 gtgcgaatgc tcgatcacca gggcaaggaa gtcctgccag ccgaattcat ggcggccgcc
     2221 gagcgcaacg acctgctcaa gaacatcgac cgctgggtgg tgggcgcgtc gctgtcgttc
     2281 gccgcgaaga tgaagccggg cgcgctgttc gtgcgccttt cgcgcgacag cgtgctcgac
     2341 gcgggattca ccgactggct caagacccag ctcgccaatg gcacgcacca tcccgaacgc
     2401 atctgcttcc agatcaccga gagcatcttc gagcagtaca tgaacgccgc ggtcacgcag
     2461 ctcggcgcgc tcgagaaaat gggcttccgc ctcgcgctcg agcggttcgg ctcgggccgc
     2521 gacccggtca aactactcac ggcagtgccg ctcgacttca tcaagatcga cggcgcgctg
     2581 gtgcaggacc tcgccggcaa tttcgagacg cagaccatgg tcaagaaact ggtcgaacac
     2641 gcgggcaagc tcaagatcga gacgatcgcc gaacgcgtcg aagacgccaa caccatggcg
     2701 gtgctctggc agctcggcgt gcagttcatc cagggatact tcgtcaacgc gccggaagaa
     2761 gtggtcatca ccgatggcca gggccgccgc taacccccgc atgtcgaaat tcctgctgct
     2821 cgcgatgacg atgctgccgc tcgcggcaac ggccgcggcg ccgcctgcgg tcgagcgcaa
     2881 catcgtctac ggcatgtact cgggtctcgc gctgctgctc gacgtacacc ggcccgcgaa
     2941 gccaaacggt ttcggcgtga tcttcatttc cggcagcggc tggcaatccg ggctcgagta
     3001 cggcgccgcg cccctcaagg aacagcaggt tccgctctgg ggcccgccgc tgctctcggc
     3061 cggttacacg gtgttcgcca tcaatcatcg ggcctcgccg cgatttcatt accccgccgc
     3121 ggtcgatgac gtgcaacgcg ccatccgctt cgtgcgattc cacgcgcgcg actacggaat
     3181 cgacggcacg cgactgggtg gcgtcgccgg ctcctccggc gcgcacttgt taggactggc
     3241 cgccatgctg ggcgccgcgg gcaatccgca ggataccgac cccgtgaatc gcgaatccgc
     3301 gacgctcgcg gccgtggtgc tgcgggcacc gccgctcgat ctgcgcacca tcgacacccc
     3361 ggaaggcacg gcctacatcg tgtcgttcat ggaagcgccg aagtcgaatg cctcggccgc
     3421 gcaatcgtac gcggcggcct cgcccatcac gcacgtgggc gcacgctcgc cgccggtgtt
     3481 gctgctgcat ggcgacgcgg acacggtcat tccggtcgat cagtccgtcg cgatgaaggc
     3541 cgcgctcgaa gcagccaggg tgccggtgaa actggtgacg attccgggcg gcatacatgg
     3601 cgcggacttc ggcgccgcgc aaaaacgggc cgactggccg gactacttcg gcgaaaccat
     3661 cgcctggctc gaccgctacc tgcgcggaaa atagcccgcg atctacttcg gccctgcttc
     3721 gttcgagtag atagccgtgg ccgcccctac ggcgctggtc acccagccgc gatacatgcc
     3781 actcgagttc atcgcgaaag cgatgttgcc ctggccgtcc agggcgatca ggccaccgtc
     3841 tcctccgatg gatccgatgt cggcgatcgt agcggacgcc gccacctgca cgctgtcacc
     3901 gcgccaggcg atgcgatcgc agagctgccg ggccgcgctc gtacgtatga aaaattctcc
     3961 cgaaccggtg gctgataccg cgcagttgcc atctgccgcg taggtgcccg cgcccacgac
     4021 cggcgaatcg ccgatcctcc cccagcgctt gccggtcatg ccgcccgtcg acgtgcccgc
     4081 ggccacgtgt ccgttcacgt cgatggccac ggcgccgacc gtcccgcgcg aatgcgtggg
     4141 gtccagcaat tgggcgttgt cacgccgcca gtcgagcagc tgctgccaac gctgctcggt
     4201 gcggaaatag cccgggtcga cctgttcgag cccttgctcc accgaaaact ggtcggcgcc
     4261 ttcgcgcgcc agcatgacgt gccgtgactt ctccatgacc gcgcgcgcca ggctgatggg
     4321 attgcgcgtg cgcgtgacgc cggccacggc gcccgccttg cgcgtcgacc cgtccatgat
     4381 cgacgcatcg agctcattgc gtccctcggc ggtgaacacc gcgccacgtc cggcgttgaa
     4441 caacgggtcg tcttcgagca ggcggatcac ggcctcgacc gcatcgaggc tcgaaccgcc
     4501 gcgcttcagc accctggcgc ccgcgcccat ggcattgagc atcgcggcgc gataagcgac
     4561 ttccttctcg ggcgtcaggt cgccgcgttc gatgacgcca gcgccgccgt gtatggcgag
     4621 cgcccagcgt ggcgccgcct ggcgttcggc ggtttgcgca ggcgtgacgg atgccgtcat
     4681 cgaacagacc agcagaaaca gtacgcgggt acgcggggcc atgtcctcat cccgggactc
     4741 gccgaagtgg cgccgcgcga ttatgcgcgc gaggtcgacg gcgcgcccct gccgctccta
     4801 gaattgccac gaccgaggtg aaccatgacg atctacggac ttctcacatt ctgcgcggtg
     4861 tatgcgctcg cggtcgccgc gcccgggccc ggcatcgcgg ccatcgtcgc gcgcggcctc
     4921 gcgcacggca cgaagggcgc gcccgcgttc attggcggat tcatcgtcgg cgacctcctc
     4981 tggttcgcga tcgccgcgac cggcctcgcg gccctggcga ggaccgcggc ggcgctcttc
     5041 gtggccatca agtgggccgg cgtcgcctat ctactgtacc tggcgtacaa gctgtggacc
     5101 gcgccggcga agcgcgtcga agtcgatgcc gccgaccgca agcagcatgg ctggaatgcc
     5161 ttcctcgcca gcctgatgct cacgctggcg aatccgaagg ccatcctgtt cttcctcgcg
     5221 ctgctgccca ccgtcatcga ccttgagtcg atgaatacgg tgcgcttcct cgagatctcg
     5281 gcggccatca tcgtggttca gccgctggtg ctcggctccg atgcgttact cgcggcaaga
     5341 gcccgcgagc tgttcaccac gccgaaggcg gtccagagac tgaaccgcag ttcgggtatc
     5401 gcgatggcta gcgccgcggt ggtcgtcgcg acgcgctagg actactcggt ctcgagcagg
     5461 tcgacggtgc gccagcctcg cggcagcacg gagccgcgct gcgcgcgctg accgcggtaa
     5521 tccttgaggt ccttccagtc gatcgtcatc ttgcgatcgc cggatctgac caccagcgac
     5581 ttgtccttcg gcaccaccgt catgccggtg aggaagtcct cgcgcgactc gaacttcttc
     5641 gacgggatgt cgaacagctt gttgcccttg ccgcgggcga gctcgggcac gtccttgacc
     5701 gggaacacga gtagtttgcc gccctccgat gtcaccgcga ccagcgcatc gtcaccgggc
     5761 accggcgccg gcggcagcac gagtgcgcca tcgggaatgt tcaacaacgt cttgccggcc
     5821 ttcttgctcg cgtaggcttc tttcaggcgg accgtgaaac catagcccgc gtcgctcgcg
     5881 accagccaga gatcctccgg ctcgccgatc atcacgccgc gccacttcgc gccatcgggc
     5941 ggatcgacgc gcgaggacag cggctcgccc aatccgcgtg ccgatggcag cgagtgcgcg
     6001 accaggctgt acgcgcgccc ggtggaatcg aggaacaccg cctgctgaac gctgcgcccg
     6061 cgcgccgcgt gctggtattc gtcgcccgcc ttgtaagtga gcgagcgcgc atcgatctcg
     6121 tgccccttgg ccgagcgcac gaagccgccc ttcgacagca cgatggtggc cggctcattc
     6181 gcgaccagct cggattcgtc gatggcctgc gccgcttcgc gctcgacgat cttcgtacgg
     6241 cggtcatcgc cgtatttctc cgcatccgct tcgatctcgc tggccacgag cttcttgagt
     6301 cgcgccttcg atttgaggat gccctcgagt tcttcgcgct cttcggcgag cttcttctgc
     6361 tcgtcgcgga tcttcatctc ttcgagcttg gcaagatggc gcagcttggt ttcgagaatc
     6421 gcctcggcct ggatctcggt gagcttgaac cgcttcatga gcacgggctt gggctcgtcc
     6481 tcacggcgga tgatgcggat cacctcatcc aggttgagat acgccgccag caggcc
//
LOCUS       DATDEU010000284         2927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_780765, whole genome shotgun sequence.
ACCESSION   DATDEU010000284 DATDEU010000000
VERSION     DATDEU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780765"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="moaA"
                     /locus_tag="VIV63_06895"
     CDS             <1..1054
                     /gene="moaA"
                     /locus_tag="VIV63_06895"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY6124360.1"
                     /translation="GENIVPLSALGQEKFPLDTLRRPIHDLRISVMDRCNFRCPYCMP
                     RETFHESYRFLKSNERLDFVELQRLARVFVRAGVKKLRITGGEPLLRPNLSDFIGDLT
                     SLPGIEDVALTTNGILLAKYASELKAAGLKRITVSLDTLDPDIFAKMSGGFGGVAEVL
                     DGIEHARRAGLAPVKINTVVQRGVNDHTVLDLLAHFRGTGVIVRFIEYMDVGTRNHWN
                     PALVVPSRELAAMIHARWPIAPREREYRGEVAERHAYVDGAGELGFISSVSQPFCGDC
                     SRARISSDGSFYTCLFATQGVDLRGPLRAGATDDEMFELIRGVWTGRRDRYSELRASM
                     RGSSELHKIEMNYIGG"
     gene            1059..1565
                     /gene="moaC"
                     /locus_tag="VIV63_06900"
     CDS             1059..1565
                     /gene="moaC"
                     /locus_tag="VIV63_06900"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY6124361.1"
                     /translation="MPRPRQKLTHLDAANRPTMVDVGAKTASSREATAEAIVKLPPAV
                     ARELARTGHRTKKGPVFDTAIVAGVMAAKRTHEIIPFCHPLALERCSVDIASGAAGRI
                     RIVCSVAVHHKTGVEMEALMGATAAALTIYDMCKALSHDIVIEGVQLLRKTGGKRDFQ
                     RTARRKKK"
     gene            1562..2164
                     /locus_tag="VIV63_06905"
     CDS             1562..2164
                     /locus_tag="VIV63_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY6124362.1"
                     /translation="MNIPRIHGLVLAGGKSSRMRRDKAAIEFRAGETQLDAAMRLLEG
                     RVARSYVSVRADQQDEARSRYPRIIDRGDAEGPIAGISAAFASYPDAAWLVLACDLPF
                     LDAQTLDTLLLARDPKFDATAFRSSHDGLPEPLCAIYEPQSRKALDAQIAAGRNCPRK
                     FLINAHTQLLEQPNPRALDNVNTVEEYEHAMSALKQTSAR"
     gene            2170..2418
                     /locus_tag="VIV63_06910"
     CDS             2170..2418
                     /locus_tag="VIV63_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY6124363.1"
                     /translation="MPELTIRVQYYALLREQAGRSDETLTTGARDARELYAELSERYP
                     FTLPAEMLRVAINAEFGEWSQPLKAGDAVVFIPPVAGG"
     gene            2415..>2927
                     /locus_tag="VIV63_06915"
     CDS             2415..>2927
                     /locus_tag="VIV63_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY6124364.1"
                     /translation="MNPFQFSTTPLDTAALQREMRDVTCGGFAAFEGWVRDHNEGMVV
                     TRLEYEAYAELAEKEGARIVQDAIAKFGVTRAACVHRVGSLDLTDVAVWVGVSAAHRD
                     EAFRACRYIIDEVKHRVPIWKKEHYENGDSGWVNCERCAAPAAHESGYAAPAAHEREY
                     AAPMAYARQTA"
BASE COUNT          538 a         1017 c          919 g          453 t
ORIGIN      
        1 cggcgaaaac atcgtccccc tgtccgccct cggccaggaa aaattcccgc tcgacacgtt
       61 gcgccggcct atccacgacc tgcgcatctc ggtcatggac cgctgcaatt tccgctgccc
      121 ctattgcatg ccgcgcgaaa cgttccacga gtcataccgg ttcctgaagt ccaacgagcg
      181 gctcgatttc gtcgaactgc agcggctggc gcgcgtgttc gtgcgcgccg gcgtgaagaa
      241 gttgcgcatc acgggcggcg aaccgctgct gcgcccgaac ctttcggatt tcatcggcga
      301 cctcacgtcg ctgcccggca tcgaggacgt tgcgctcacg accaacggca tcctgctggc
      361 caaatacgcg agcgaactca aggcagcggg cctcaagcga attaccgtca gtctcgacac
      421 gctcgacccc gacatcttcg cgaagatgag cggcggcttc ggcggcgtgg ccgaagtgct
      481 ggacggcatc gaacacgcgc gtcgcgcggg actcgcgccc gtcaagatca acaccgtcgt
      541 gcaacggggt gtcaacgatc acaccgtgct cgacctgctc gcgcatttcc gcggcaccgg
      601 cgtcatcgtg cgcttcatcg agtacatgga cgtcggcacg cgcaatcact ggaatcccgc
      661 gctcgtggtc ccgtcgcgcg aactcgcggc gatgatccac gcgcgctggc cgatcgcgcc
      721 gcgcgagcgc gaataccgcg gcgaagtcgc cgagcgtcat gcctacgtgg atggcgcggg
      781 cgaactcggc ttcatctcct cggtctcgca gcccttctgc ggcgactgtt cgcgcgcacg
      841 catttcctcg gacggatcgt tctacacctg cctgttcgcc acccagggtg tcgatctgcg
      901 gggtccgctg cgcgccggcg ccaccgacga cgaaatgttc gaactcatcc gcggggtctg
      961 gaccggtcga cgcgaccgtt acagcgaact gcgcgcctcg atgcgcggct cttccgagct
     1021 gcacaagatc gagatgaatt acatcggcgg ttgatcccgt gcccagaccc agacaaaaac
     1081 tgacccatct cgacgccgcg aaccgcccca ccatggtgga cgtcggcgcc aagaccgcga
     1141 gttcgcgcga agcgaccgcg gaggccatcg tcaaactacc gccggcggtg gcacgcgagc
     1201 tcgcgcgcac cggccatcgc acgaagaaag gcccggtgtt cgacaccgcc atcgtcgcgg
     1261 gcgtcatggc ggcgaagcgc acgcacgaga tcatcccgtt ctgccatccg ctggcgctgg
     1321 agcgttgctc cgtggacatc gccagcggcg cggccggacg catacgcatc gtctgcagcg
     1381 tggccgtgca tcacaagacc ggcgtcgaga tggaggcgct catgggcgcc accgccgcgg
     1441 cgctcaccat ctacgacatg tgcaaggcgc tgtcacacga catcgtcatc gaaggcgtcc
     1501 aactgctgcg caagaccggc ggcaagcgcg acttccagcg caccgccagg aggaaaaaga
     1561 aatgaacatc ccgcgcatcc acggactggt gctggccggc ggcaaaagct cgcgcatgcg
     1621 gcgcgacaag gccgcgatcg aatttcgcgc cggcgaaaca cagctcgacg ccgccatgcg
     1681 cctgctggaa gggcgcgtcg cgcgctccta cgtttcggtg cgcgccgatc agcaggatga
     1741 ggcgcgctcg cgttacccgc gcatcatcga tcgcggcgat gcggaaggcc ccatcgccgg
     1801 catcagcgcc gcctttgcga gctacccgga cgccgcatgg ctggtgctcg cctgcgacct
     1861 gccctttctc gacgcgcaga cgctcgacac cctgctgctc gcgcgcgatc cgaaattcga
     1921 cgcgacggcg tttcgttcca gccacgacgg cctgcccgaa ccgctgtgcg cgatctacga
     1981 gccgcagtcg cgcaaggcgc tcgacgcgca gatcgccgcc ggccggaatt gtccgcggaa
     2041 attcctcatc aacgcacaca cgcaactgct cgagcagccc aatccgcgcg cgctcgacaa
     2101 cgtgaacacc gtggaagagt atgagcacgc catgtcggcc ctgaagcaaa cttccgcccg
     2161 ttgagcgcca tgcccgaact gaccattcgc gttcagtact acgccctgtt gcgcgaacag
     2221 gccgggcgca gcgatgagac cctcactacc ggcgcgcgcg atgcgcgcga gttgtacgcg
     2281 gaactgagcg agcgatatcc gttcacgctg cccgcggaga tgctgcgcgt agccatcaac
     2341 gcggaattcg gcgaatggtc gcagccgctc aaggccggcg atgccgtcgt cttcattccc
     2401 ccggtggccg gcggatgaat ccctttcagt tcagcaccac gccgctcgac accgcggcgc
     2461 tgcaacgcga gatgcgcgac gtcacctgcg gtggattcgc ggccttcgaa ggctgggtgc
     2521 gcgatcacaa cgaaggcatg gtcgtcacgc gcctcgaata cgaggcgtac gcggaactcg
     2581 ccgaaaagga aggtgcgcgc atcgtgcagg acgccatcgc gaagttcggc gtgacgcgcg
     2641 ccgcctgcgt gcatcgcgtg ggttcgctgg acctgaccga tgtcgccgtg tgggtgggcg
     2701 tcagcgcggc gcatcgcgac gaggcattcc gcgcgtgccg ctacatcatc gatgaagtca
     2761 aacatcgcgt accgatctgg aagaaagagc actacgaaaa cggcgactcg ggctgggtga
     2821 actgcgagcg ttgcgccgcg ccagcggcgc acgaaagcgg atacgccgcg ccagcggcgc
     2881 atgaacggga gtacgccgca ccgatggcat acgcgcgcca gaccgcg
//
LOCUS       DATDEU010000285         9935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_196655, whole genome shotgun sequence.
ACCESSION   DATDEU010000285 DATDEU010000000
VERSION     DATDEU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196655"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..785
                     /locus_tag="VIV63_06920"
     CDS             219..785
                     /locus_tag="VIV63_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124365.1"
                     /translation="MKAILLGVFAVAAVSACTVNGQGPMTAWGKPNVSKVDYGTDVGM
                     CTGLAAMQGSGNGANTAGGINGSNGSARSTNGGDAAVAGGQTGGGATGAPTTIGGGTY
                     QGMASQDMVQRAATQESSQAMAARLAREATLKHCLVERGYQEFRLTPEQRSHLATLKV
                     GSNEYHEYLYALGADASVLNKQGLASTK"
     gene            complement(786..2342)
                     /locus_tag="VIV63_06925"
     CDS             complement(786..2342)
                     /locus_tag="VIV63_06925"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6124366.1"
                     /translation="MSRRAIKCRVTSEKSALTEPAGDARKRLQDIVSKALGGNFAEAE
                     AGARALLDKPVAAQAWSALSEIYANTQRWTEARMALDVALQQSPDSRDLRQRRALLLE
                     KQGRDDEALTELSTLAAEAMDSPQLLVHLGRALLFAGRAVEAITLLEGGIERWPTDAH
                     VHRLLAHAYWQRGDGESCTRELERAIARFPVELPLRLVAADVQRNAGNPRKALELLQP
                     GVDIAPNHPALLTSMGVLLDLLDRPDEALPLLRAAVASAPNSGQAKRNLITTLLRVSA
                     HDEAATLCDELLAGSPDDQLLIAHRATAYRQADDARYAWLHDYDRLVRAYRLTPPPEF
                     AGIAGFNTQFANRLELLHRAQRHPLGQSLFGGTQTDRNLPADDPLVAQFLAMIDAPIR
                     DYISRLRANDREHPADRRVTRDYRIAGSWSVQLKPGGFHTSHVHPQGWISSAYYVELP
                     AASPGDAARSGWLKFGEVPSSALEWPANHFIEPEPGMLVLFPSYLWHGTVPFENGGRR
                     LTAAFDVVPA"
     gene            2512..5724
                     /locus_tag="VIV63_06930"
     CDS             2512..5724
                     /locus_tag="VIV63_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6124367.1"
                     /translation="MSAKKSRISELSILALGATLLAPYAIAQDQDAAATNTATTAKSD
                     EDLTEILVTGIRKALQTSAEIKKESDTVVDSITASDIGAFPDKSVAEALQRMSGITVT
                     RFAASGDTTHFSAEPSGVVIRGLPQVRSEFNGRDSFNANSSRGLSFADVSPELMAGVD
                     TYKNSTAEMIEGGIAGTVNLRTHVPFDSEGFVAAFSGELGYGDLAEETKPAGSMLISS
                     RWETGIGDIGLMANAAYSEVVTESQGVQLGRFYRVDGVAAYGGGTKWMSGGVDIRQNT
                     YTRTRKGGSLAAQWASPEDTLLATLQYNRSEYENDWEEYSLSAGIGNSQTAQDLVLTS
                     QLGFAPAGTPAYEFDSRGVFTSGVINDNSGNWAGQTNPTMGHPNGVYNNGTYLEWYCH
                     SWQGNDVDGDACPATRGIGLSADTRSSTSENITEDLSLNLRWALNDRVGLNFDIQKID
                     STVVNFDNSTNNKTATDLYMDLSGGKPRFEFRAPTGFGFTAGGFGDPQNWYHEWEMEH
                     AEDSKGEELAYRLDADIKLSEGGWMDSLRVGVRRAERDQDVNWSTYNWASVQPLWGIE
                     QDTPLFLNGGAWAGSYEAKDLGSELVGGGVFGGGTFLHPAFAVVSDYQATIDLYGNGI
                     SHEWVPLAQRTNSPRPASQCPVIPGTLFCPAEVLGVNEDTSAAYVMLKFGGDDTKWGN
                     LPVKGNIGVRYVETDVNSTGSIQYPGFTRPIPDPGAPSVPIDFLVPIADAQFMNYGSF
                     AQSGGSKTEHWLPSLNVRLGLTDDQFLRFAASRAMARPDMGLYKYYYGISMVENNCTS
                     GTVTYSEPGNCNLPSVPVSVTPRYTASVGNPFLKPTTADQFDFTYEWYFSNVGQFTAA
                     LFYKKFNDYVTSASYVQDFTNNGVTRPVTITAPVNTDGASLKGFELAYQTFFDRLPSP
                     WNGFGIQTNFTYVDNQGVNNAGLTSVSGGGGTQQDPLITFTDLPLEGFSDTSYNIVLM
                     FEKEKYSARLAYNWRSEYMISQSDCCIKLPIWQDAYGQLDGSFHFKPSGNWDVYLDLQ
                     NILKAETVLRQQVNQDGLLLPRSWFTNDRRISLGLRYRIQ"
     gene            5807..7351
                     /locus_tag="VIV63_06935"
     CDS             5807..7351
                     /locus_tag="VIV63_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6124368.1"
                     /translation="MRTPLNIVIVGGGTAGWMTAAAFASVYPAAQRRIRLIESSEIGI
                     VGVGEATLPHIRFFNKRIGINEPELMAKTQATFKLGIEFADWARIGDSYIHPFGAYGS
                     RIAGVPFHHYWLRQQQQGDTTPIEDYSLAIVASRQNRFAMPSEDPSAVLSTFGYAFQF
                     DASLYAAYLRTFAEARGVQRTDGKIVDVNLRGEDGWIESVKLNDGSVVAGDLFIDCSG
                     FRGLLIEQALKTGYDEWTRWLPCDRAAAVPCENEGPLPPYTRATAREAGWQWRIPLQH
                     RTGNGYVYSSNFISDDEAGSKLMARLPGKALADPRFLRFTAGRRRKTWNRNCVAIGLS
                     GGFLEPLESTSIYLIQIAITTLIDYLVDRPDGADSSHYDPRIVDAFNRWIEMEYDRVR
                     DFLILHYHATERNDAPIWDYCRTMDIPDSLKHKMELFRERARVVTYKDGLFLEPSWLA
                     VYFGQRVMPRGYDSLADAAQGDELSKHLQGVREQIRNAVAHMPRHEDFLQAYCPAGAA
                     PARSTM"
     gene            7348..8838
                     /locus_tag="VIV63_06940"
     CDS             7348..8838
                     /locus_tag="VIV63_06940"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6124369.1"
                     /translation="MSVQNVVVVGGSPSAWLAACVLRRAFRHRDLDVLVVDPGASADA
                     PSGRWTLPSLRGLHALVGINENDFLRQTGASYKLAVEHCDWQGPGSHFVHAHAEIGTG
                     AETLPFYKYLLAQRLHGRAANPEDYSVGAAAARAGKFARPLSDGPALAKNFTYAFHLG
                     EAAYLAYVRAHAAKLGVRHIAGTVAEVALREDGGIDAVILEDGARVTGGFFIDCSGAK
                     EVLMQRLPSSGIDDWSQWLPCDRMWSGLATALPEPKALTTTVATDAGWTWSAPLANGR
                     IAGYVYSSAIRDDDAAMRELSAAVGGQEISAPSLRTLRSGRRREFWVKNCVALADTAV
                     TLEPLAGADLHLAQLGIVNFVELFPLGAGAVEAVEYNRLMGEHADALRDFTLAHYRLN
                     RRAGSFWNSVRAKALPDRLAHKLDLFAASGRIILLDQESFEEVDWAWVLLGSGLTPAA
                     LELQTRTLLDPVTDAQVAPMRATVQQLVASMPRHIDYLSRQGPARP"
     gene            8835..>9935
                     /locus_tag="VIV63_06945"
     CDS             8835..>9935
                     /locus_tag="VIV63_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6124370.1"
                     /translation="MNRTVARIVIVGGGTAGWMAAAACSKILDTRKYPVEVIESDEIG
                     TVGVGEATIPPIMDFNRTLELDEAEFVRATQASFKLGIEFVDWTRLGDSYLHPFGPYG
                     VPMHGIFFHHFWLRYRRDGGKLPNSAFNVSATAAREGNFSPPNPQERSPLPPHAYAYH
                     FDAGLYAAYLRKYAEARGVVRHEGRIVDVRLHGEDGFIESVVTTDGRIIAGDLFLDCS
                     GFRGLLIEQALKSGYEDWREWLPCDRAMAVPCERLATTTPFTRSTAREAGWQWRIPLQ
                     HRIGNGYVYCSNYLPDDEAARLLLTRLDGQTSAEPRPLRFIAGRRREVWKKNCVALGL
                     ASGFLEPLESTSIHLVQSAIARLMFLFPGQGFD"
BASE COUNT         1789 a         3287 c         3141 g         1718 t
ORIGIN      
        1 tcgccgcgac cggcaatctg tgcggagtca tcgacaaggc gcgcgccgac cgggccgccg
       61 ccggtagctg aaggcccgtt ccgggccccg cctgaccgga cggttgtacg ggattgcccg
      121 tggcgggtat cttgcgtccc ccgcggcggc aggaatcttt ttgcatcggc cgcggtcata
      181 catgggagcc ggatgtcacg gcagcagggg agaatccgat gaaagcgatt ttgttaggcg
      241 tgttcgcggt ggccgcggtc agtgcgtgca cggtcaatgg ccagggcccg atgacggcct
      301 ggggcaaacc gaatgtgtcg aaggtcgatt acggcaccga tgtcggcatg tgcacgggcc
      361 tcgcggccat gcagggcagt ggcaacggcg cgaataccgc cggcggcatc aacggctcga
      421 acggttcggc gcgttccacc aacggcggtg acgccgcggt ggcgggcggt cagaccggcg
      481 gcggcgccac gggcgctccg acgaccatcg gcggcggcac ttaccaggga atggcgagcc
      541 aggacatggt ccagcgcgcc gcgacccagg aaagctccca ggccatggcc gcccggctgg
      601 cgcgtgaagc aacgctcaag cactgcctgg tcgagcgcgg atatcaggaa ttccgtctga
      661 cgcccgagca gcgctcgcac ctcgccacgc tcaaggtcgg cagcaacgaa taccacgagt
      721 atctctacgc gctcggcgcc gatgcctcgg tgttgaacaa gcagggtctc gcttcgacga
      781 aatagttagg caggcaccac atcgaaggcg gccgtcagcc ggcggccgcc gttttcgaaa
      841 ggcacggtgc cgtgccacag gtacgacggg aacagcacca gcattcccgg ttccggctcg
      901 atgaaatgat tcgccggcca ttcgagcgct gacgacggca cctcgccgaa cttcagccat
      961 cctgaccgcg ccgcgtcgcc tggcgacgcc gccggcaatt ccacgtagta agccgagctg
     1021 atccagcctt gcggatgcac gtggctggta tgaaaaccgc cgggcttcag ctgcaccgac
     1081 cacgagcccg cgatgcggta atcccgcgtc acgcggcgat cggcgggatg ttcgcgatcg
     1141 ttcgcgcgca gtcgcgaaat gtagtcgcga atcggcgcgt cgatcatcgc caggaactgc
     1201 gcgaccagtg gatcatccgc tggcaggttg cggtccgtct gcgtgccgcc gaacagactc
     1261 tggccgagcg gatgccgctg cgcgcggtgc agtagttcga gacggttcgc gaattgcgtg
     1321 ttgaagccgg caattcccgc gaattccggc ggcggcgtga gccggtatgc gcgcaccagc
     1381 cggtcgtagt cgtgcaacca cgcgtatcgc gcgtcgtccg cctgccggta cgcggtcgcg
     1441 cgatgcgcga tcagcagttg atcgtcgggc gaccccgcga gtagttcgtc gcagagcgtc
     1501 gcggcttcat cgtgcgcgga cacacgcagc agcgtcgtga tcaggttgcg cttcgcctgt
     1561 cccgagttcg gcgcactcgc cacggctgcg cgcagcagcg gcagcgcttc atcggggcgg
     1621 tccagcagat ctaacaacac gcccatcgat gtgaggagcg cagggtggtt cggcgcgatg
     1681 tcgacaccag gctgcagcag ttcgagcgcc ttgcgcggat tgccggcatt gcgttgcacg
     1741 tcagccgcca ccagccgcaa tggcaactcg accgggaacc tcgcgatcgc acgttcgagc
     1801 tcccgtgtgc aggactcgcc gtcgccgcgc tgccaatacg catgggctag caacctgtgg
     1861 acgtgcgcat cggtgggcca ccgttcgatg ccgccttcca gcaacgtgat cgcttcgaca
     1921 gcgcggcccg cgaacagcag cgcgcggccg agatggacta acaactgcgg cgaatccatc
     1981 gcctcggcgg ccagcgtgga caattccgtc agcgcttcgt cgtctcgtcc ctgcttttcc
     2041 agcaaaagcg cgcgtcgttg acgcaaatcc cgggaatcgg ggctttgttg cagcgcgaca
     2101 tcgagcgcca ttctggcctc agtccagcgt tgcgtatttg catagatttc actcaatgcg
     2161 ctccacgcct gggcagcgac aggtttgtcc agaagggcac gcgcgcccgc ttcggcctcg
     2221 gcgaaatttc cgccaagcgc tttcgaaaca atatcttgca ggcgctttcg cgcatcgccg
     2281 gccggctcag tcaaagcgct tttctcactc gtgacgcgac attttatggc tcgccgagac
     2341 atgcggcccg gacacaactt ttggcaaata gggtcttgct tgtgcgcgcg atctcgctag
     2401 actccaaaaa aattctaaca atcggtttcc gaattaatta gctcgtaata gtttctatac
     2461 agggggggca ttgcagtgtc caaagcatct aaaagattgc ggcgtgaagc cgtgagcgcg
     2521 aagaagtcgc gcatctctga attatccatc cttgctctcg gcgccacgct cctcgcacct
     2581 tatgcgatag cgcaggatca ggacgccgcg gcaaccaaca ccgccacgac ggccaagagc
     2641 gacgaagatc tcaccgagat cctcgtcacc ggcattcgca aggcgctcca gacctcggcg
     2701 gaaatcaaga aggaatcaga cacggtcgtc gattccatca ccgcgtccga catcggcgcg
     2761 ttccccgaca agagcgtggc cgaagccctg cagcgcatga gcggtatcac cgtcacgcgc
     2821 ttcgcggcgt cgggcgacac cacccacttc tccgccgaac cttcgggcgt cgtcatccgc
     2881 ggtctgccgc aggtgcgcag cgagttcaac ggccgtgaca gcttcaacgc gaacagctcg
     2941 cgcggtctgt cgttcgccga cgtctcgccc gaactgatgg ccggcgtgga cacctacaag
     3001 aactcgaccg cggaaatgat cgaaggcggc atcgccggca cggtcaatct gcgtacgcac
     3061 gtgcccttcg actccgaagg cttcgtggcg gcgttctccg gcgaactggg ttacggcgac
     3121 ctcgccgaag agaccaagcc cgcgggttcc atgctgatca gcagccgctg ggaaaccggc
     3181 atcggcgaca tcggcctcat ggccaacgcc gcttactccg aagtcgtcac cgaatcgcag
     3241 ggcgtgcagc tcggccgctt ctaccgcgtc gacggcgttg ccgcgtacgg cggtggcacc
     3301 aagtggatgt ccggcggtgt cgacattcgc cagaacacct acacgcgcac gcgcaagggt
     3361 ggatcgctgg ccgctcaatg ggcgagcccc gaagacacgc tgctcgccac cttgcagtac
     3421 aaccgttccg agtacgaaaa cgactgggag gaatattccc tcagcgccgg tatcggcaac
     3481 tcgcagacgg cgcaggacct ggtcctcacc tcgcagctcg gcttcgcacc ggcgggaacg
     3541 cccgcgtacg agttcgacag ccgcggcgtg ttcacgagcg gtgtgatcaa cgacaactcg
     3601 ggcaactggg cgggtcaaac aaacccgacc atggggcacc cgaatggcgt gtacaacaac
     3661 ggcacgtacc tcgagtggta ttgccattcg tggcagggca acgacgtcga cggcgacgct
     3721 tgccccgcga cccgcggcat tggcctcagc gccgacacgc gttcgtccac gtccgagaac
     3781 atcaccgaag atctttcgct gaacctgcgc tgggcgctca acgaccgcgt cggcttgaac
     3841 ttcgacatcc agaagatcga ctcgaccgtc gtcaacttcg acaacagcac gaacaacaag
     3901 acggcgaccg acctgtacat ggacctctcg ggcggcaagc cccgattcga gttccgcgca
     3961 ccgacgggct tcggcttcac ggccggcggt ttcggcgatc cgcagaactg gtaccacgaa
     4021 tgggaaatgg agcacgccga agacagcaag ggtgaagaac tcgcctaccg tctcgacgcc
     4081 gacatcaagt tgagcgaagg cggctggatg gactcgttgc gcgtaggcgt acgccgcgcg
     4141 gaacgcgatc aggacgtcaa ctggtcgacg tacaactggg cttcggtgca accgctctgg
     4201 ggtatcgagc aggacactcc gctgttcctg aatggtgggg catgggctgg ctcctatgaa
     4261 gcgaaggacc tcgggtccga gctcgtcggc ggcggcgtct tcggcggtgg cacgttcctg
     4321 cacccggcct tcgccgtggt gagcgactac caggcgacca tcgacctgta cggcaacggc
     4381 atcagccacg agtgggtgcc gctggctcag cgcaccaatt cgccgcgtcc ggcgtcgcag
     4441 tgtccggtca tccctggcac gctgttctgc ccggccgaag tgctgggtgt gaacgaagac
     4501 accagcgccg catacgtcat gctgaagttc ggcggcgacg acaccaagtg gggcaacttg
     4561 ccggtcaagg gcaacatcgg cgttcgctat gtcgaaaccg acgtgaactc gacgggcagc
     4621 attcagtacc ccggcttcac gcgtccgatc ccggacccgg gtgcaccttc cgttccgatc
     4681 gacttcctcg ttccgatcgc cgacgcacag ttcatgaact acggttcgtt tgcgcaatcc
     4741 ggcggtagca agaccgagca ctggctgccc agcctgaacg tgcgattggg cctcacggac
     4801 gatcagttcc tgcgctttgc cgcgtcccgt gcgatggctc gccccgacat gggcttgtac
     4861 aagtactact acggcatctc gatggtggag aacaattgca cctcgggtac ggtcacgtac
     4921 tccgaacccg gcaattgcaa cctgcccagc gtgccggtct cggtcacgcc gcggtacacg
     4981 gccagcgtgg gtaatccgtt cctcaagccc accacggctg accagttcga cttcacgtac
     5041 gagtggtact tctcgaacgt gggtcagttc acggcggcgt tgttctacaa gaagttcaac
     5101 gactacgtga cctcggcgag ctacgtgcag gacttcacca acaacggtgt gacgcggccg
     5161 gtgaccatca ccgcgccggt gaacaccgac ggcgcaagcc tcaagggctt cgagctggct
     5221 taccagacgt tcttcgatcg tctgccctcg ccgtggaacg ggtttggcat ccagaccaac
     5281 ttcacgtacg tggacaacca gggcgtgaac aacgccggcc tgaccagcgt gtcgggtggt
     5341 ggtggtacgc agcaggatcc gctgatcacg ttcacggatc tgccgctcga aggcttctcg
     5401 gatacttcgt acaacatcgt gctgatgttc gagaaggaga agtactcggc gcgtctcgcc
     5461 tacaactggc gctcggagta catgatcagt cagtctgact gctgcatcaa actgcccatc
     5521 tggcaggatg catacggcca gctcgacgga tcgttccact tcaagccgag tggcaactgg
     5581 gacgtctatc tcgacctcca gaacatcctc aaggccgaga cggtgctccg tcagcaggtc
     5641 aaccaggatg gcttgctgct gccgcgttcg tggttcacga acgatcgccg aatctcgctg
     5701 ggcctgcgtt accgcatcca gtaattcgcg aagctgagaa gaccgggcgc gccgtatacg
     5761 atacggcgcg cccttttctt ttcccgaccg aggagctccg tcgcccgtgc gtaccccgct
     5821 caacatcgtc atcgtcggcg ggggcactgc aggctggatg accgccgcgg cgtttgcctc
     5881 tgtttacccg gcggcgcagc ggcgcatccg tctcatcgaa tccagtgaaa tcggcatcgt
     5941 cggcgtaggc gaagccacgc tgccgcacat ccgcttcttc aacaaacgaa tcgggatcaa
     6001 cgagcccgag ctcatggcca agacccaggc cacgttcaag ctcggcatcg aattcgccga
     6061 ctgggcgcgc atcggcgaca gctacatcca cccctttggc gcctacggct cgcgcatcgc
     6121 cggcgtgccg ttccatcact actggctgcg ccaacagcag cagggcgaca ccactcccat
     6181 cgaggactac tcgctggcca tcgtggcgtc gcgccagaac cgttttgcca tgccgtcgga
     6241 agatccgtcc gcggtgctct ccacgttcgg ctacgcgttc cagttcgatg cgtcgctgta
     6301 tgccgcctat ctgcgcacgt tcgccgaggc gcgcggcgtg cagcgcaccg acggcaagat
     6361 cgtcgacgtc aatctgcgcg gcgaagacgg ctggatcgaa tccgtgaagc tcaacgatgg
     6421 ctcggtcgtg gccggcgacc tcttcatcga ctgctcgggt ttccgcgggc tgctcatcga
     6481 gcaggcgctg aagaccggct acgacgaatg gacgcgatgg ctgccgtgcg atcgcgccgc
     6541 ggcggtaccc tgcgaaaacg aaggaccgtt gccgccgtac acgcgcgcca cggcgcgcga
     6601 agccgggtgg caatggcgca ttccgctgca gcaccgcacc ggcaacggtt atgtctacag
     6661 cagcaacttc atcagcgacg atgaggccgg atcgaaactc atggcgcggc tgccgggcaa
     6721 ggcgctcgcc gatccgcgtt tcctgcgttt caccgcgggc cggcgccgca agacgtggaa
     6781 tcgcaactgc gtcgccatcg gtctctcggg cggattcctc gagccgctcg agtcgaccag
     6841 catctatctg atccagatcg cgataaccac gctcatcgac tatctggtgg accgcccgga
     6901 cggcgcggac agcagtcact acgatccgcg catcgtcgac gccttcaatc gctggatcga
     6961 aatggaatac gaccgcgtgc gtgatttcct gatcctgcat taccacgcga ccgagcggaa
     7021 cgacgcgccg atctgggact actgccggac gatggatatc cccgacagcc tcaagcacaa
     7081 gatggaactc ttccgcgagc gcgcgcgcgt cgtcacctac aaggacggac tgttcctcga
     7141 gccgagctgg ctcgcggtgt acttcggtca gcgcgtcatg ccgcgcggct acgattcgct
     7201 ggccgatgcc gcccagggcg atgagctctc gaagcacctg cagggtgttc gcgaacagat
     7261 ccgcaacgcc gtggcgcaca tgccgcgcca cgaagatttc ctgcaagcct attgccccgc
     7321 gggcgccgcg ccggcacgga gcacgatgtg agcgtgcaaa acgtggtcgt ggtgggcgga
     7381 agtccctcgg cctggctcgc ggcctgcgtg ttgcggcgcg cgttcaggca tcgcgatctc
     7441 gacgtgctcg tggtggatcc gggcgcgagc gccgatgcgc cgtcgggacg ctggacgttg
     7501 ccgtcgttgc gcggtctgca cgcgctggtc ggtatcaacg agaacgactt cctcaggcag
     7561 accggtgcct cctacaaact ggccgtcgaa cactgcgact ggcaggggcc gggcagtcat
     7621 ttcgtgcacg cgcatgcgga gatcggcacg ggcgccgaaa cgctgccttt ctacaagtac
     7681 ctgctcgcgc aacggcttca cgggcgcgcc gcgaatcccg aggactattc ggtcggagcg
     7741 gccgccgcgc gtgccggaaa attcgcacgt cctctcagcg acggacccgc gctcgcgaaa
     7801 aacttcacgt atgcgttcca ccttggtgag gccgcctatc tggcctatgt gcgtgcgcat
     7861 gcggcgaagc tcggcgtgcg gcacatcgcc ggcaccgtcg cggaagtcgc gttgcgcgaa
     7921 gacggcggaa tcgacgcggt cattctcgaa gacggcgcgc gcgtgaccgg cggattcttc
     7981 atcgactgtt cgggcgcgaa agaagtcctc atgcagcggc tgccgtcatc gggcatcgac
     8041 gactggtcgc aatggctccc ctgcgatcgc atgtggtcgg gactcgcgac ggcgctgccc
     8101 gaacccaagg ctttgacgac gacggtggcg accgatgccg gctggacctg gagcgcgccg
     8161 ctcgccaacg gccgcatcgc cggctatgtc tactcgagcg cgatccgcga tgacgacgcg
     8221 gcgatgcgcg agctctcggc cgccgtcggt ggccaggaga tcagcgcgcc ttcgctgcgc
     8281 acactgcgct caggacgccg tcgcgagttc tgggtgaaga attgcgtggc gctggcggat
     8341 acggccgtca cgctcgagcc gctggcaggc gccgacctgc acctggcgca actcggcatc
     8401 gtgaacttcg tggaactgtt cccgctcggc gccggcgccg tggaggcggt cgagtacaac
     8461 cgattgatgg gcgaacacgc cgatgccctg cgcgacttca cgctcgcgca ctatcggctc
     8521 aaccggcgcg ccgggagttt ctggaactcg gtgcgcgcca aggcgctgcc cgaccggctg
     8581 gcgcacaagc tcgacctgtt cgccgcgagc gggcggatca tcctgctcga ccaggaatcg
     8641 ttcgaggaag tggattgggc gtgggtgctg ctcggcagtg gcctcacgcc cgcggcgctg
     8701 gagctgcaga cgcgcacgct gctcgacccc gtcaccgatg cgcaggtggc gccgatgcgc
     8761 gcgacggtgc agcagctcgt ggcatcgatg ccgcggcaca tcgactatct ttcccggcag
     8821 gggcccgcgc gaccatgaac aggacagtag ccaggatcgt gatcgtcggt ggcggaaccg
     8881 ccggctggat ggccgccgcc gcctgctcga agattctcga cacccgcaaa taccccgtcg
     8941 aagtcatcga gtccgatgag atcggcaccg tcggcgtcgg cgaagccacc attccgccga
     9001 tcatggattt caatcgcacg ctggaactcg acgaagccga gttcgtgcgc gcgacgcagg
     9061 cgagcttcaa gctcggcatc gagttcgtcg actggacgcg tctcggcgat tcctatctgc
     9121 atcctttcgg gccctatggc gtgccgatgc acggaatctt cttccaccat ttctggctgc
     9181 ggtaccgccg cgacggcgga aaacttccga acagcgcgtt caacgtgagt gccaccgcgg
     9241 cgcgcgaagg caacttctcg ccgccgaatc cgcaggagcg atcgccgctg ccgccccatg
     9301 cgtatgcgta ccacttcgac gccggcctgt acgccgcgta cctgcgcaag tacgccgagg
     9361 cgcgcggtgt cgtgcgtcac gaaggacgca tcgtcgacgt ccgtctgcac ggcgaggatg
     9421 gtttcatcga gtccgtcgtc accaccgacg gccgcatcat tgccggcgac ctgttcctcg
     9481 attgctcggg ttttcgcgga ctgctcatcg agcaggcgct gaagtcgggc tacgaggact
     9541 ggcgcgagtg gctgccctgc gatcgcgcca tggcggtgcc ctgcgagcgt ctcgccacca
     9601 ccacaccgtt cacgcgctcc acggcgcgcg aagccggctg gcagtggcgc atcccgctgc
     9661 agcaccgcat cggcaacggc tacgtgtact gcagcaacta tctacccgac gacgaggccg
     9721 cgcgcctgct gctgacgcgc ctcgacggtc agacgtccgc cgagccgcgc ccgctgcggt
     9781 tcatcgccgg gcggcggcgc gaagtgtgga agaagaactg cgtggcgctc ggcctggcca
     9841 gcgggttcct cgagccgctc gagtcgacca gcattcacct ggtgcagtcg gccatcgcgc
     9901 gcctcatgtt cctgttcccc gggcaggggt tcgat
//
LOCUS       DATDEU010000286         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_342546, whole genome shotgun sequence.
ACCESSION   DATDEU010000286 DATDEU010000000
VERSION     DATDEU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342546"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /locus_tag="VIV63_06950"
     CDS             <1..1909
                     /locus_tag="VIV63_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEY6124371.1"
                     /translation="AETMSAILREDPPDPSTLNVTVAPGVLRALRRCLEKRPQERFDS
                     ARDLAFALESAVDSSSASGAAALPPLRDRRWLVGAVAAVTLGAVLGFIGARAIGRSAA
                     ASDTSLQAQFRRLTFDKGTIPDGRFTPDGQSIIYGAAWNGQPLKLFMVRSDNPESAPL
                     SLPDARLLSVSKTGELAISLGHTYEGWMGEGTLARSSLLGTAPRVMAEHVREAEWTPD
                     GSDLAVVRRVDGFERLEFPLGKVLYQTSGFISDIRFSPSGDRIAFADHPLYSDDAGLI
                     AVVDRDGHRTALSDDGWTSLHGLAWTRDGSEIWFGATRGVDVNATGIYAVTLGGQLRP
                     VIAGPTRYKLLDIASNGRVLVSHDRDDRVVEGLMAGSATPVDISLRANSSANWVAEDG
                     RSTLIGDLSAASYQTYFLKAGATPVKLGTGTPTSLSPDGRWAVAIPVEGRPLFLHPTG
                     PGESRKLPDPDNIVFDNVGWLDTTHIIGFGQKIGERSQGYIQDISGGPPRRFTPEGAT
                     VKALKWWTLPISSDGTRVIAMNGHDAVEIYPVNGGAPQPVPHLKTGDVVVQWSADDRG
                     LLVAHRDGLPWVVERLDLASGQRTPAATIRAHDAAGLRLSIFGISRDAKYFVHTYARL
                     LSDLYVVDGLK"
     gene            2052..>2403
                     /locus_tag="VIV63_06955"
     CDS             2052..>2403
                     /locus_tag="VIV63_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY6124372.1"
                     /translation="MPTRSQQSRVELLQGTLDLLILRTLRWGPAHGHAIAKAIEHGSD
                     DVLLVEQGSLYPALHRLIERGWITFDDGTSENNRRAKFYRLTAKGRKQLAIETSKWDT
                     LAAAVGRILRPAAQE"
BASE COUNT          405 a          778 c          783 g          437 t
ORIGIN      
        1 agcggagacg atgtcggcga ttctgcgtga ggatccgccc gacccgtcga cgctcaacgt
       61 gacggtcgcg ccgggtgtgc tgcgcgcgct tcgacggtgt ctcgagaagc gtccgcagga
      121 acgattcgac tcggcgcgcg atctggcgtt tgccctggag tccgcagtcg acagcagcag
      181 cgcatcgggc gccgcggcgt tgccgccgct ccgcgatcga cgatggctgg tcggcgccgt
      241 cgctgccgtg acgctcggcg ctgtcctcgg cttcatcggc gcgcgcgcaa tcggtcgttc
      301 ggcggccgca tccgacacgt cgttgcaggc ccagtttcgc cgcctgacgt tcgacaaggg
      361 tacgatcccc gacgggcggt tcacacccga cggacagtcg atcatctatg gcgccgcgtg
      421 gaacggccag ccgctcaagc tgttcatggt ccggtccgac aacccggaat cggcccctct
      481 gtcgttgccc gatgcacggt tgttgtcggt gtcgaaaacg ggcgagctgg cgatttcgct
      541 cggtcacacc tacgaaggat ggatgggcga gggcaccctc gcgcgatcgt cgctgctggg
      601 cacggcgccg cgcgtgatgg ccgagcatgt tcgcgaagcc gaatggaccc cggatggcag
      661 cgatctggcg gtcgtgcgtc gcgtcgacgg cttcgaacga ctggaattcc cgctcggcaa
      721 agtcctgtat caaacatccg gtttcatcag cgacattcgc ttctcgccat cgggcgatcg
      781 catcgccttt gccgatcatc cgttgtactc cgacgatgca ggactcatcg cggttgtcga
      841 tcgagacggc caccgcacgg cattgtcgga cgacggctgg acatctctcc acgggcttgc
      901 gtggaccaga gacggatcgg aaatctggtt cggtgcaacc aggggtgtgg acgtcaacgc
      961 gaccggcatc tatgcggtga cgctgggcgg acagttgcgt ccggtgattg ccggccccac
     1021 gcgctacaag ctcctggaca tcgcgtcgaa cggtcgggtg ctggtcagcc atgatcgcga
     1081 tgaccgcgtg gtcgagggat tgatggccgg cagcgcgacg ccagtggaca tctcgctgcg
     1141 ggcgaattcc agcgcgaatt gggtggccga ggacggtcgc agtacgctca tcggcgacct
     1201 gtctgcggcg tcataccaga cgtacttcct caaggcgggc gccacgcccg tcaaactcgg
     1261 gaccggcaca ccaacatcgc tgtctcctga tggtcgctgg gcagtggcca taccggtcga
     1321 aggccgccca ctgtttctgc atcccaccgg tcctggcgag tcgcgcaagc ttcccgaccc
     1381 tgacaacatc gtgttcgaca atgtcggctg gctggatacg acccacatca tcggtttcgg
     1441 ccagaagatc ggcgagcgct cgcagggcta cattcaagac atcagcggtg gtccgccgcg
     1501 gcgtttcacg cccgaagggg cgacagtcaa ggcgctcaag tggtggacgc tgccgatttc
     1561 ctcggatggc acgcgcgtca tcgcgatgaa cggccatgac gcggtcgaga tctacccggt
     1621 caacggcggt gcgccgcagc ctgttcctca cctcaagact ggcgacgtcg tcgtgcaatg
     1681 gagcgcggac gaccgcgggc tgctggtggc acatcgtgac gggctgccgt gggtcgtcga
     1741 gcggctggac ctcgccagcg gtcagcgaac gcccgcggcg acgattcgtg ctcacgacgc
     1801 ggccgggttg cgcctgagca ttttcgggat ctcgcgcgac gcgaaatact tcgtccacac
     1861 ctacgcgcgt ctcctgtccg atctctacgt cgtcgatgga ttgaagtaga ccgaggcgca
     1921 tcgcatcgcc ccttccggtc cgatctcaca cgtgtcgcgg cgtaccgcct ggttgcgacc
     1981 gggttgcgga atgcctgccg gttcgcacac cttgtcgttc taggtgaatc gatgtatcgt
     2041 cgtcacctag aatgcctacc agaagccagc agagccgcgt cgaactcctg cagggcacgc
     2101 tcgacctcct gattctgcgc acgctgcgat ggggacccgc ccacggccac gccatcgcga
     2161 aagcgatcga acatggctcg gatgacgtgc tcctggtgga acagggctcc ctctatccag
     2221 cccttcaccg gctgatcgaa cgtgggtgga tcacgttcga cgacggcact tccgagaaca
     2281 atcgacgcgc caagttctat cgactgacgg cgaagggtcg gaagcagctc gcgatcgaaa
     2341 ccagcaagtg ggacacgctc gcggcagccg tggggcggat cctccgccct gctgcccagg
     2401 agg
//
LOCUS       DATDEU010000287         6647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1656310, whole genome shotgun sequence.
ACCESSION   DATDEU010000287 DATDEU010000000
VERSION     DATDEU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656310"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1150
                     /gene="hemH"
                     /locus_tag="VIV63_06960"
     CDS             137..1150
                     /gene="hemH"
                     /locus_tag="VIV63_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY6124373.1"
                     /translation="MKWGVLLVNSGTPASLATRDVRAFLAGLLGDRRVVELPRLLWWP
                     ILHGIILRVRPRASAKKYAAIWTPQGSPLAIESEALRLGLEKSLHENEIPVALAMLYT
                     GGASVPDAIEALREAGADGILVIPMFPQYCGASTGAVYDQVSAALGEMRHVPSLRFVS
                     SYHDDAAYIEALAASVRDHQREFGVTSHLLMSFHGVPERFVTQGDPYRDQCHRTAILL
                     AQRLGLSDEAWSVSFQSRFGRARWLQPYTSEVLAAFPQRGVKSVSVICPGFVADCLET
                     LEEIGMENRDVFLAAGGEKYHYIAALNARADHIAALGALISRELGVSPSPALGKEPSA
                     CFG"
     gene            1138..1569
                     /gene="hemJ"
                     /locus_tag="VIV63_06965"
     CDS             1138..1569
                     /gene="hemJ"
                     /locus_tag="VIV63_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEY6124374.1"
                     /translation="MLWLKAFHIVGVVSWFAALLYLPRLFVYHAQIQGASIDDDAGNA
                     RFKIMERKLFMIMTIGAVVAVCFGVAMLAMAPDYLLMRWLHVKLALVLILVGYHGACF
                     ALLQDFAADRNSHSERWYRLFNEVPALLLIAIVILVVVKPF"
     gene            1686..1874
                     /locus_tag="VIV63_06970"
     CDS             1686..1874
                     /locus_tag="VIV63_06970"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEY6124375.1"
                     /translation="MRTSHIEESPPAATPPSTARPASERTVSTKELLGGAHRLWIQHA
                     AERYLLQVTRTGKLILTK"
     gene            1992..2303
                     /locus_tag="VIV63_06975"
     CDS             1992..2303
                     /locus_tag="VIV63_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124376.1"
                     /translation="MRIPVIGRVPATAVHHDIERARSVVLQCSHTLFRDARDADAWHA
                     LGVALVSLGDRVAAFIALRNAALLDGRRANTQLALGNLLFDSGRVDDAVRCFECVESE
                     G"
     gene            2400..4142
                     /locus_tag="VIV63_06980"
     CDS             2400..4142
                     /locus_tag="VIV63_06980"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY6124377.1"
                     /translation="MRATVQLAAACLTVTAFLVGCSGEREAKPAESAAPAAANMDAGW
                     PAFVDAFIEARFKADPYFAVQAGRHEYDGQMPDWSRAALDADVAELRKSATALAAFDP
                     MTLTPGQRFEHEYLQWVIDSQLFWVASAESPYRNPAWYLDKLDPSMYLTREYAPLPKR
                     LEGFLGYSRAIPALAANIRANLRSPLPRAFIERGAAGFGGYATFFRGEMPAVFAQLTD
                     EKLKADLIAANAAAATAMEELTAWLESQRSAATEDFALGSARFLEMLRQTERVDITLE
                     DLELVGRKDLERNLAALKEACGAFAPKATLSACVEKMRANKPSGGTVEGARAQLAELR
                     QLVLDKKLVTIPSQEQALVAESPPYNRGNFAYISIPGPYENPAVKVTYYVAPPDARWS
                     AAERNAYLPGKAYLLFVSAHEVWPGHYLQSQYMNANPSRTLALWWDYAFGEGWAHYTE
                     EMMYDAGLGAGQPEMRVGMLTNALLRDVRFLSAICLHSGCMSLEESEKMFREQAFADA
                     GNARQQALRGTYDPGYLAYTLGKLMIRKLRADWLAANAGATPQQFHDKFLSFGAPPIP
                     LVRNQMLGTSEGAL"
     gene            4316..4702
                     /locus_tag="VIV63_06985"
     CDS             4316..4702
                     /locus_tag="VIV63_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124378.1"
                     /translation="MGVALAQVQASSSVYMIDVDPARYSGPAGQRVILRVGDAPRHVT
                     IGTQDEICVQLQRVEGNSEVISARVRALRPEPQTATDVRLVGPNISPGTYRSTPWGLL
                     LRAMPGKVEVPGVSAQGTDNAPVCTL"
     gene            complement(4752..5624)
                     /locus_tag="VIV63_06990"
     CDS             complement(4752..5624)
                     /locus_tag="VIV63_06990"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6124379.1"
                     /translation="MRVAFLGLGKMGSGMAARLLAAGHSLAVYNRTPARAAPLAKLGA
                     RVADTPGAAAHKADVIISMTADDESSRALWLGEQGALAADNVPHALAIECSTLSHDWV
                     LELAVKVRERKFRFVDSPVTGLPDAAANGTLTLLVGADAADLDAARPIFASLATRVLH
                     FGAVGQGTAYKLMVNLLGAVQIASAAEAIVLAERAGLDLKLVTDAVASGQAASPQVVR
                     NVRRFVAGDHGTNVNFTPALRLKDIEYALRLAAKLGVDCPFGNVAAELYRKLCAQGGG
                     GDNESRIIEALRSS"
     gene            complement(5624..>6647)
                     /locus_tag="VIV63_06995"
     CDS             complement(5624..>6647)
                     /locus_tag="VIV63_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124380.1"
                     /translation="GQPAHAAPDHVDITWMSIANMHFDLGAQQILADGYITRIPAEIF
                     HGGASGFAATYRATRPDDAAVREVFDAMGGKSSFNLLLTGHSHFDHSFDTATWAQFSG
                     ARIIGSPTTCLQTRAAKIPADRCTPVYGGEKFTLQPGVDVYVIRWNHSGDPARNPEQH
                     NPVELSAVPKPDSAGAMRAGLAEDFPNGGGNRAYLFTVDGPQGRFSWLFQDSGSAVDL
                     RDPVVVGGKDFGAPLENLRAAMKAAKLESVDLWIATGGLDVASLVLPVVKPKAYLPVH
                     WDGLFGAFKAGPPGAYSDPALDAVLVKQGIRQIVPTQYMDKWRLDRQGVRALDNSAIK
                     RKLGFN"
BASE COUNT         1168 a         2136 c         2071 g         1272 t
ORIGIN      
        1 cgctgctcat gtcgatgccg ccacgtgcgg agtggcgcta cgactatcgt ccggcgccgg
       61 gttcgcccga agcggcgctg gccgactacc tgaagccgcg cgactgggtg gcgtgagcag
      121 ccatgaggat atcgtcatga aatggggcgt cctgctcgtg aattccggta cgccggcgtc
      181 gctggcgacc cgtgacgtgc gcgcgtttct cgccgggttg ttaggcgacc ggcgcgtggt
      241 cgagctgccg cgtctgctgt ggtggcccat cctccatgga ataatcctgc gcgtccgccc
      301 acgcgcgagc gcgaagaagt acgccgccat ctggacaccc cagggctcgc cgctcgccat
      361 tgaaagcgag gcgcttcgcc tgggtctcga gaaatcactg cacgagaatg aaatccccgt
      421 agcacttgcc atgctctaca ccggtggcgc cagcgtgccc gatgccatcg aggcgcttcg
      481 cgaggcgggc gcggatggca tcctcgtcat cccgatgttt ccgcagtact gcggtgcttc
      541 gacgggcgcc gtgtatgacc aggtcagcgc tgccctcggc gagatgcgcc acgtcccttc
      601 gctgcgattc gtttccagct accacgatga cgccgcgtac atcgaggcac ttgccgcgag
      661 tgttcgggat catcagcgcg agttcggtgt gacgagccat ctgctcatgt cgtttcacgg
      721 cgtgcccgag cgattcgtga cgcagggcga tccgtatcgc gatcaatgcc atcgcacggc
      781 catactgctc gcacagcggc ttggcctcag cgacgaggcg tggagcgttt ccttccagtc
      841 gcgctttggc cgggcacgtt ggctgcagcc ctataccagc gaagtactcg ccgcctttcc
      901 ccagcgcggc gtgaaatccg tgagcgtcat ctgccccggg ttcgtggccg actgcctcga
      961 gaccctggag gagatcggca tggagaatcg ggacgttttc ctcgcggccg gcggcgagaa
     1021 ataccactac atcgccgcgc tcaatgcgcg tgcggatcat atcgctgcac tgggcgcatt
     1081 gatttcgcga gagctgggtg tttcaccgag ccccgccctg ggcaaggaac cttccgcatg
     1141 ctttggctga aggcgtttca catcgtcggc gtggtgagct ggttcgcggc gctgctctac
     1201 ctgccgcggc tgttcgtcta ccacgcgcaa atacagggcg cttccatcga cgatgatgcg
     1261 ggcaacgcgc gcttcaagat catggaacgc aagctgttca tgatcatgac catcggcgcc
     1321 gtcgtggcgg tctgtttcgg cgtcgccatg ctggccatgg cgcctgacta tctactcatg
     1381 cggtggctgc acgtgaagct ggcgctggtg ctgatcctcg tgggatatca cggcgcctgt
     1441 tttgcgctgc tccaggactt cgcggccgac cgcaattcgc actccgagcg ctggtaccgg
     1501 ctgttcaatg aagtacccgc gctgctgctg atcgccatcg tcatcctggt ggtcgtgaaa
     1561 cctttctgac ttaacgcgcg ggcgagtgga cttgtaactc actgttttag caggggcttg
     1621 caatgttcac ggaccggttc tattctctgg ccgcaaatga gaagcgttct tatttgaggt
     1681 ctcgaatgcg caccagccat atagaagagt cgcctccggc ggccacaccg ccttctaccg
     1741 cacgacccgc atcggagcgg acggtctcca ccaaagagtt gttgggcgga gcccaccgcc
     1801 tgtggatcca gcacgccgcc gagcgctacc tgttgcaggt cacgcgcacc ggcaaactga
     1861 tcctgaccaa gtagcgccga atactaacta ctcttccgtt ccggtcagcc acccaggcac
     1921 tgccgagccc ccgctcccag tttccccagg cagccaaccc tcgcgcgtca cgcgctttcc
     1981 gagaggttgg catgagaatt cccgtcatag gccgcgtgcc ggcgactgcg gttcaccacg
     2041 acatcgagcg tgcgcgcagc gtggtgttgc agtgctcgca caccctgttc cgggacgctc
     2101 gtgatgccga tgcctggcac gctctcggtg ttgcgctcgt gtctcttgga gatcgagtcg
     2161 ccgcgttcat cgcgctgcga aatgccgcgt tgctcgatgg aagacgcgcg aatactcagc
     2221 tggcgctcgg taatctgctg ttcgacagtg gacgcgtcga cgatgcggtg cgctgctttg
     2281 agtgcgtcga aagcgaaggg tgattccgct ttccaatgcc ggtgccgtca tgcgcaaacc
     2341 ggcgcccgcc cgctgattca tttacgatcg atccccacca accttgtggg agatcgagaa
     2401 tgcgcgccac ggtccagctt gctgctgctt gcctcactgt caccgcgttc ctcgtgggtt
     2461 gctccggtga acgcgaggcc aagcctgcag aaagcgcggc tccagccgcc gccaacatgg
     2521 acgccgggtg gcccgcattc gtagatgcat tcatcgaagc gcgcttcaaa gccgatccgt
     2581 acttcgcggt tcaggcggga cgccacgaat acgatggcca gatgccggac tggagccgcg
     2641 cggccctgga cgcggacgtc gcggagctgc gcaaatcggc tacggcgctc gccgctttcg
     2701 atcccatgac gctgacgcct ggccagcgat tcgaacacga atacctgcaa tgggtgatcg
     2761 actcacaact cttctgggtc gcgagtgccg agtcgccgta tcgcaacccg gcctggtatc
     2821 tcgacaagct cgatccgtcg atgtacctca cacgggagta cgcgccgctg ccgaaacggc
     2881 tcgaaggctt tctcgggtac tcgcgcgcga ttccggcact cgccgcgaat attcgcgcca
     2941 acttgcgctc gccattgccg cgagccttca ttgaacgagg agccgccggc ttcggtggct
     3001 acgcaacctt cttccgcggc gaaatgccgg ccgtttttgc gcaactcacc gacgagaaac
     3061 tcaaggctga tctgatagcg gcgaatgccg ccgccgctac cgcgatggaa gagctgaccg
     3121 cgtggctgga gtcgcagcga agtgccgcca cggaagactt cgcgctcggc tcagcgcggt
     3181 tcctggagat gcttcggcag acggagcgcg tcgacatcac gctcgaagac ctggagctcg
     3241 tgggccgcaa ggatctcgag cggaatctcg cggctctcaa ggaggcttgt ggagcattcg
     3301 cgccgaaagc aacactctcc gcgtgcgtcg agaaaatgcg cgctaacaaa ccatcgggcg
     3361 gcacggtcga aggtgcgcgc gcccaactgg ctgagctgcg ccaactcgtg ctcgacaaaa
     3421 aactggtcac tatccccagc caggaacagg cgctcgtggc tgaatcgccg ccgtacaatc
     3481 gcggcaactt cgcttacatc agcatccccg gtccctacga gaatccggcg gtgaaggtca
     3541 cgtattacgt ggcgccccct gacgcccggt ggagcgccgc ggaacgtaac gcctatcttc
     3601 ccggcaaggc ctatcttctt ttcgtctccg cgcacgaggt atggccgggc cactatctcc
     3661 agtcgcagta catgaacgcg aatccgtccc ggacgctcgc tctatggtgg gactacgcgt
     3721 tcggcgaagg ctgggcgcac tacacggaag agatgatgta cgacgcgggg ttgggcgccg
     3781 gtcagccgga gatgcgcgtg ggcatgttga ccaatgcgct gctgcgcgac gtgcgatttc
     3841 tctcggcaat ctgtctgcac agcggctgca tgtcgctcga ggaatcggaa aagatgtttc
     3901 gtgagcaggc cttcgcggat gcgggcaatg cgcggcagca ggcgctgcgc ggcacctacg
     3961 accctggtta tctggcgtac acgctgggca aattgatgat ccgcaagttg cgagccgact
     4021 ggctcgcggc gaatgccggc gctacgccgc agcaattcca cgacaagttt ctctcttttg
     4081 gtgcgccacc cattccgctc gtgcgcaatc agatgcttgg cacgtccgaa ggtgcactct
     4141 gaagtgacgc actaactaca cgtttgtgac tttttgtcgc gaagcgtgac gttgtgtatg
     4201 tcggtggacg ccgacaggat gtcagcgtat atgctgaggg aacttcttac gcctaaagtg
     4261 ctccgatacc tttatgcgca aaacagtttt atccgcggcc atctgtgctg cactcgtggg
     4321 agttgcgctc gcgcaggttc aggcgagcag cagcgtgtac atgatcgacg tcgacccggc
     4381 gcgttactcg gggcccgctg gtcagcgagt catccttcgc gtcggtgatg ctcctcgaca
     4441 cgtaaccatc ggcacgcagg acgaaatctg tgtgcagctg cagcgcgtcg aaggaaactc
     4501 ggaagtcatc agtgcgcgag tgcgtgcatt gcgtcccgaa cctcagaccg caaccgatgt
     4561 acggctggtc ggccccaaca tctctcccgg cacctaccgc agcacgccgt ggggcttgct
     4621 gctgcgcgcc atgccaggaa aggtcgaggt acccggcgtc agtgcgcagg gcaccgacaa
     4681 cgccccggtc tgcacgctct gacgagttcc ggcctgatga ggtctggcct gatcaggcga
     4741 agccgtgcgc gctaggaact ccgcagcgcc tcgataatgc ggctctcgtt gtcgccaccg
     4801 ccgccttgcg cgcagagttt gcggtagagc tcggccgcta catttccaaa cgggcaatcc
     4861 acgcccagct tggctgcgag tcgcagcgcg tactcgatat ctttcagccg cagcgcagga
     4921 gtgaagttga cgttcgtacc gtgatctccg gccacgaaac gccgtacatt gcgcacgacc
     4981 tgcggactcg ccgcctggcc actcgcgacc gcgtcggtca ccaacttcag atccaatccc
     5041 gcacgctccg ccagaacgat tgcttcggcg gcagatgcga tctgcaccgc gccgagcagg
     5101 ttgaccatca gtttgtaggc cgtgccctgg ccaacggcgc caaagtgcag cactcgtgtc
     5161 gcgagcgacg cgaagatcgg acgggcggcg tcgaggtcgg cggcgtcggc gcccacgagc
     5221 aacgtgagcg tcccgttcgc cgccgcatcc ggcaggcccg ttacgggtga atccacaaac
     5281 cggaattttc tctcgcgcac tttgaccgcg agctccagca cccagtcatg cgacagcgtc
     5341 gagcactcga tggcgagtgc atgcgggaca ttgtccgccg ccagggcacc ctgttctccg
     5401 agccacaggg cccgtgaaga ttcgtcgtcg gcggtcatgc tgatgatgac atcggccttg
     5461 tgcgcggcag cgcccggggt gtccgcgact cgcgcgccga gtttcgcgag aggtgccgcg
     5521 cgcgccggcg tccggttgta caccgccagc gagtgacccg ccgcgagcag gcgcgcggcc
     5581 atgccgcttc ccatctttcc gagcccgagg aacgcaacgc gcatcagttg aaacccagtt
     5641 tccgtttgat cgccgaatta tccagtgcgc ggacaccctg gcggtcgagc cgccacttgt
     5701 ccatgtactg cgtcggcacg atctggcgaa ttccctgttt caccagcacc gcatcgagcg
     5761 cggggtcgga ataagcgcct ggcggccccg ccttgaaggc accgaacaac ccatcccagt
     5821 gcacaggtag ataggccttc ggcttcacga ccggcagcac cagcgacgcc acgtcgagcc
     5881 cgccggtcgc gatccacaaa tcgactgact ccagctttgc cgctttcatc gccgcgcgca
     5941 ggttttcgag cggagcaccg aagtccttgc caccgaccac gacagggtca cgcagatcca
     6001 cggcgctgcc cgaatcctgg aaaagccagc tgaagcggcc ttggggtccg tcgacggtga
     6061 acagataggc gcgattgccg ccgccattcg gaaagtcctc ggcgagccct gcgcgcatgg
     6121 ctccggcgga atccggtttc ggaacggcac tgagctcgac cggattgtgt tgttcgggat
     6181 tccgcgcggg atcgccgctg tggttccagc ggatcacgta cacgtcgacg ccgggttgga
     6241 gcgtgaattt ctcgccgccg tacaccggcg tgcagcggtc ggcagggatc ttcgcggcac
     6301 gagtctgcag acatgtggtt ggcgagccga tgattcgagc gcccgaaaac tgagcccagg
     6361 tcgcggtgtc gaacgagtgg tcgaagtggc tatggcccgt gagcagtaga ttgaaggagg
     6421 acttcccgcc catcgcgtcg aagacttcgc gaacggccgc atcgtctggc cgtgtagcac
     6481 ggtaggtcgc ggcgaatccg ctggcgccgc cgtgaaagat ctccgccggg atgcgcgtga
     6541 tgtatccgtc agcgaggatc tgttgcgcac ccaggtcgaa atgcatgttg gcgatcgaca
     6601 tccacgtgat gtcgacgtga tcgggcgccg cgtgtgctgg ttggcca
//
LOCUS       DATDEU010000288         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_197283, whole genome shotgun sequence.
ACCESSION   DATDEU010000288 DATDEU010000000
VERSION     DATDEU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197283"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VIV63_07000"
     CDS             <1..767
                     /locus_tag="VIV63_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124381.1"
                     /translation="RKVNGAFVTLAERPLALALNTKHRLRLSVAGSRIDVEVDGASML
                     SANDTSFSRGRAALLTYRARADFDNVVASPTAPLNVNFNDWVSYWYGFGRPFTEVGGN
                     WEITGQENPEGLSQTNTTDLAYAINGVATDTQVIRAHIRLDSFAAQSGAWFGLLARYV
                     DEGNHYFLSIRSSNQLQIRKVVNGVTTVLRAVAFTPAPGAMREYTFSVLGNELHAYVD
                     GQLVATALDDSLPRGRYGMGTFRAAATWQDFSVDQP"
     gene            complement(764..2074)
                     /locus_tag="VIV63_07005"
     CDS             complement(764..2074)
                     /locus_tag="VIV63_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6124382.1"
                     /translation="MIKLTRYQWTVFFAAWLGWGFDIFDGLLFNFVAPNAIPTLLDIP
                     IGGPEAKAAVPYWNGILNSILLLGWAAGGVIFGPLSDRFGRRRVLMITMLLYALGTAC
                     CAFVTEMWQLIVFRIIASLGIGGEWAAGSAMVAEVVPEDKRVEAGALLYTSAPLGLFL
                     ADFVNREVAGDWFLHDPSMSWRYVLMFGLIPAAVAFLVRAFIKEPERWAAAAATAAPA
                     RVREIFAPDIRRRTFSALIVAVIALITWWTCNAFIQAMTNSLAGAESAARGLDATATA
                     VLKRDWMTLATYSFCWGGLIGTMLTIPLAKMVGRRTMYAVYFAGSALALLTTFGLDLP
                     AETRLRMYFFIGLTVFGVFGSFTFYLPELFPTRLRSTGSGFCYNIGRVVTAGGVFAVG
                     MVQQVAKGDPGVFVNTMFMIGFVPILGLLLLPWVIETRGQKMPD"
BASE COUNT          431 a          727 c          724 g          360 t
ORIGIN      
        1 agcgcaaggt gaacggcgcg ttcgtgacgc tcgccgagcg gcccttggcc ctggcgctca
       61 acacgaaaca tcgcctgcgg ctttcggtcg ccggctcgcg catcgacgtc gaagtcgatg
      121 gcgccagcat gctgtccgcg aacgacacgt ctttttctcg cggccgcgcc gcgcttctca
      181 cctaccgcgc gcgtgcggac ttcgacaacg tcgtcgccag cccgaccgcg ccgctgaacg
      241 tcaacttcaa cgactgggtg tcctactggt acggtttcgg ccggccgttc acggaagtcg
      301 gcggcaactg ggagatcacc ggccaggaga atccggaagg cttgtcgcag accaacacca
      361 cggacctcgc gtacgcaatc aatggtgtcg ccacggatac ccaggtgatc cgcgcccaca
      421 tccgcctcga ttcattcgcc gcgcagtcgg gtgcgtggtt cggcctgctc gcgcgatacg
      481 tggacgaggg caatcactac ttcctgtcga tccgcagctc caatcagctg cagatccgca
      541 aggtcgtgaa cggtgtaacc acggtgctca gggcagtggc attcacgcct gcacccggcg
      601 ccatgcgcga atacacattc agcgtgctcg gcaacgagtt gcatgcctac gtcgacgggc
      661 agctcgtcgc gacggcgctc gatgattccc tgccgcgcgg ccggtacggc atgggcacgt
      721 ttcgcgccgc ggcgacgtgg caggacttct cggtggatca accctagtcg ggcatcttct
      781 ggccgcgagt ctcgatgacc cagggaagca gcagcaaccc gaggatggga acgaagccga
      841 tcatgaacat ggtgttcacg aacacgccgg ggtcgccctt ggccacctgc tgcaccatgc
      901 cgaccgcgaa tacgccacct gcggtgacca cgcggccgat gttgtagcag aagccgctgc
      961 cggtggagcg caggcgcgtc gggaacagtt ccggtagata gaacgtgaaa ctgccgaaca
     1021 cgccgaacac ggtgagtccg atgaagaaat acatgcgcag ccgcgtctcc gccggcagat
     1081 cgagaccgaa ggtcgtgagc agcgcgagcg cggaccccgc gaagtagacg gcgtacatgg
     1141 tgcgcctgcc caccatcttg gccagcggaa tggtgagcat cgtgccgatg agtccgcccc
     1201 agcagaagct gtaggtagcc agtgtcatcc agtcgcgctt gagtaccgcg gtggccgtcg
     1261 catccaggcc gcgcgccgcg gactcggcgc cggccaggct gttggtcatg gcctggatga
     1321 atgcgttgca cgtccaccag gtgatcaatg caatcaccgc gacgatgagc gcgctgaacg
     1381 tgcgccgtcg tatgtccggc gcgaagattt cgcgtacgcg tgccggcgcg gccgtggctg
     1441 ccgccgcggc ccatcgctcc ggctccttga tgaacgcgcg tacgaggaaa gcgaccgcgg
     1501 ccggaatgag accgaacatc agcacgtatc gccacgacat ggacggatcg tggaggaacc
     1561 agtcgccggc gacttcgcgg ttgacgaaat cggcgaggaa caggccgagc ggcgcgctgg
     1621 tgtacagcaa tgctccggcc tcgacgcgtt tatcctcggg gacgacttca gccaccatgg
     1681 ccgagccggc cgcccactca ccaccgatac ccagactggc gatgatgcgg aagacgatga
     1741 gctgccacat ctcggtgacg aaggcgcagc aggcagtgcc gagcgcgtag agcagcatgg
     1801 tgatcatgag cacgcgccgg cggccgaagc gatcgctcag cggtccgaag atcacgccgc
     1861 ccgcggccca tcccagcaac aggatggagt tcagaattcc gttccagtac ggcacggccg
     1921 cctttgcttc aggcccgccg atggggatgt ctaacaacgt gggaatcgcg ttcggcgcca
     1981 cgaagttgaa tagaagcccg tcgaagatgt cgaagcccca gccgagccag gccgcgaaga
     2041 acaccgtcca ttgatagcgc gtgagcttga tcatgcagcg cgagtgtaaa tggggacact
     2101 ccccatttcc tagcaaaagg gggctgcccc ctacgcgtgc ttgaacatga tcggaatgcc
     2161 ggacggcgtg gtgtacgtct tgccctcggt gaacgggcgg tcgcgtcctt ccggcgcgcg
     2221 gtccgccacc gcggcgagca cg
//
LOCUS       DATDEU010000289         2491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1656992, whole genome shotgun sequence.
ACCESSION   DATDEU010000289 DATDEU010000000
VERSION     DATDEU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656992"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /gene="folD"
                     /locus_tag="VIV63_07010"
     CDS             complement(<1..762)
                     /gene="folD"
                     /locus_tag="VIV63_07010"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF008058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY6124383.1"
                     /translation="MTAQILDGKAVAAKERTKSAARAADFVSRFGRSPGLAVVKVGED
                     PASAVYVRNKRKACEECGIASFAHDLPANTTREELLSLVKTLNNDYRVDGILLQLPLP
                     KGLDSTEIMDAIDPAKDVDGFHPTNTGLLAQKRPGLRPCTPWGVIKLAEEYKLDLVGL
                     RACVVGASNIVGRPMALELLLARSTVTVCHTGTRDLRGEVERAELVVAAVGKAAYIPG
                     EWIREGAIVMDVGINRGADGKLCGDVAFAAASQRAA"
     gene            complement(773..2197)
                     /gene="ahcY"
                     /locus_tag="VIV63_07015"
     CDS             complement(773..2197)
                     /gene="ahcY"
                     /locus_tag="VIV63_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY6124384.1"
                     /translation="MNAVVKPGTGADYVIADIKLAEWGRKELNIAETEMPGLMAIREE
                     YAKTQPLKGARIAGSLHMTIQTAMLIETLQALGAEVRWASCNIYSTQDHAAAAIAVKG
                     TPVFAVKGETLKDYWEYTHKIFEWPGAAEAQANMILDDGGDATLLLHLGTKAEKDQSL
                     LANPGSEEEIELFNSIKATLKKDAKWYSTRLKQIKGVTEETTTGVHRLYEMHKKGELA
                     FPAINVNDSVTKSKFDNLYGCRESLVDAIKRATDVMVAGKIAVVAGYGDVGKGSAQAL
                     RALSAQVWVTEIDPINALQAAMEGYRVVTMDYAADKGDIFVTATGNFQVINHSHMKAM
                     KHNAIVCNIGHFDNEIDVASLSKYQWEEIKPQVDHVIFPDGKRIIMLAKGRLVNLGCG
                     TGHPSYVMSSSFANQTIAQIELFTQTAKYPLGVYVLPKHLDEKVARLQLKTLNATLTE
                     LTDVQAKYIGVSKSGPYKTDHYRY"
     regulatory      complement(2220..2296)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(2311..>2491)
                     /locus_tag="VIV63_07020"
     CDS             complement(2311..>2491)
                     /locus_tag="VIV63_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HEY6124385.1"
                     /translation="SQHFDLRPKGIVQMLDLLRPIYFKTAAYGHFGRNEPEFTWEKTD
                     KAAALAADAGVRKTA"
BASE COUNT          387 a          815 c          758 g          531 t
ORIGIN      
        1 ggccgcgcgc tgggacgccg ccgcaaacgc cacgtcgccg cagagttttc cgtccgcgcc
       61 gcgattgatg cctacgtcca tgacgatggc gccttcgcga atccattcac ccgggatgta
      121 cgcggccttg cccaccgccg ccaccacgag ttcggctctt tccacttcgc cgcgcagatc
      181 acgcgtaccc gtgtggcaca ccgtgaccgt ggagcgcgcg agcaacagct cgagcgccat
      241 cggacgcccg acgatgttcg acgcccccac cacgcaggcg cgcaggccga cgaggtcgag
      301 tttgtattct tccgccagtt tgatgacgcc ccagggcgtg cacgggcgca gtcccgggcg
      361 tttctgcgct aacaagccgg tgttggtcgg atgaaatcca tcgacgtcct tggccgggtc
      421 gatggcatcc atgatctccg tgctgtcgag acccttgggc agcggcagct gcagcaggat
      481 gccgtcgaca cggtagtcat tgttcagcgt cttgacgagc gacagcagtt cttcgcgcgt
      541 ggtgttcgcc ggcaggtcgt gcgcgaacga ggcaatgccg cactcctcgc aggccttgcg
      601 cttgttgcgc acgtagaccg cggaggccgg gtcttcaccc accttgacca ccgcgaggcc
      661 cggcgaccgg ccaaacctcg aaacgaagtc ggcggctctc gccgccgact tcgttcgctc
      721 ttttgctgcg acggcttttc cgtcgaggat ctgtgccgtc atcagtaggt gatcaataac
      781 gatagtgatc ggtcttgtac gggccgctct tggaaacgcc gatgtacttc gcctgcacgt
      841 cggtgagctc ggtcagcgtc gcattcagcg tcttgagctg caggcgcgcc accttctcgt
      901 cgaggtgctt cggcaggacg tacacaccca gcgggtactt cgcggtctgc gtgaacagtt
      961 cgatctgcgc gatggtctgg ttcgcgaagc tcgagctcat cacgtagctc gggtggccgg
     1021 tgccgcaacc gagattcacg aggcgaccct tcgccagcat gatgatgcgt ttgccgtccg
     1081 ggaagatcac gtgatcgacc tgcggcttga tctcttccca ctggtacttc gagagcgacg
     1141 cgacgtcgat ctcgttgtcg aagtggccga tgttgcagac gatcgcgttg tgcttcatcg
     1201 ccttcatgtg cgaatgattg atcacctgga agttgccggt ggccgtcacg aagatgtcgc
     1261 ccttgtcggc ggcataatcc atggtcacca cgcggtagcc ttccatcgcg gcctgcagtg
     1321 cgttgatcgg atcgatctcc gtcacccaca cctgggccga cagcgcgcgc agcgcctgcg
     1381 ccgaaccctt gcccacgtcg ccgtagcctg cgaccacggc aatcttgccg gcgaccatca
     1441 cgtccgtcgc gcgcttgatc gcgtcgacca gtgactcgcg gcagccgtac agattgtcga
     1501 acttcgactt cgtcaccgag tcgttgacgt tgatcgcggg gaacgcgagt tcgcccttct
     1561 tgtgcatctc atagaggcga tgcacgccgg tcgtcgtttc ttccgtcacg cccttgatct
     1621 gcttgaggcg cgtggaatac cacttcgcat ccttcttcag cgtggccttg atcgagttga
     1681 acagctcgat ctcttcctcg ctgcccgggt tcgccagcag gctctgatcc ttctcggcct
     1741 tggtgcccag gtgcagcagc aaggtggcgt cgccgccgtc gtccaggatc atgttcgcct
     1801 gcgcctcggc agcgcccggc cactcgaaga tcttgtgggt gtattcccag tagtccttga
     1861 gcgtctcacc cttcacggcg aacaccggcg tgcccttcac ggcgatcgcc gcggcagcgt
     1921 ggtcctgcgt cgagtagatg ttgcacgagg cccagcgcac ttccgctccg agcgcctgca
     1981 gcgtctcgat gagcatcgcg gtctggatcg tcatgtgcag cgaacccgcg atgcgggcgc
     2041 ccttgagcgg ctgggtcttg gcgtattcct cgcggatcgc catgaggccg ggcatctcgg
     2101 tctcggcgat gttgagctcc ttgcggcccc actcggcgag tttgatgtcg gcaatcacgt
     2161 aatcagcgcc cgttccgggc ttcacaacag cattcatatg aacctctcta gttagttcca
     2221 tttagagagc gccgttggaa tcgatcgtcc cggccccgag cctcgcgtct tcgacgctgc
     2281 agcgctcctc ggggctggaa ctttggaaac ctatgccgtc ttgcgcactc cggcatccgc
     2341 ggcgagcgcg gcggccttgt cggtcttttc ccaggtgaac tccggctcgt tgcggccgaa
     2401 gtggccgtac gccgccgtct tgaagtagat ggggcgcagc aggtcgagca tctgcacgat
     2461 gcctttcggg cgcagatcga aatgctggct c
//
LOCUS       DATDEU010000290         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2094914, whole genome shotgun sequence.
ACCESSION   DATDEU010000290 DATDEU010000000
VERSION     DATDEU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094914"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VIV63_07025"
     CDS             <1..361
                     /locus_tag="VIV63_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124386.1"
                     /translation="IKWKDEFKQRATLFHLQPGQGAYMPSTSPHMVENDDNPSITVSF
                     TYYTDATRRNSRLHGLHQRMRNAGMSPAPVGTRAARDAVLGSLTAVSTGVRDTVARLR
                     SSNARVDTARYAHAQVG"
     gene            375..>2162
                     /locus_tag="VIV63_07030"
     CDS             375..>2162
                     /locus_tag="VIV63_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY6124387.1"
                     /translation="MSHPQSSARRTLADICAVQADSAGVAFHEVALDAPIADTLRNEK
                     DTRAALVRAWTATVAALVGEEPGDSGVWCFDGAAAPEAIVVWRATIKPTGPITLRVES
                     TRLDKNTLSALAGLLMRALAQPDSFTLLDASARETVLNGHHAPLRAIAPGDTVHALFS
                     AQAHRTPNAPAVEAGDVTLSYADLDGRSGAWAAALRERGVSAGATVAVLMQRSADAIV
                     ALLAILKAGATYLPLDQAYPPARLRFMLEDSGATLLLTDATPGDILPTGCRALAVQDL
                     AGARGSFSDAGNVSDVAYVMYTSGSTGTPKGAEIPHAAIVRLVREPGYITLDTATVFL
                     HAAPLGFDASTLEIWGPLLNGGRCVVHDEILPTGAGLAATIARHRVTSAWLTAALFNA
                     IVDENPAQLAGIAELLTGGEALSVAHVRRAYAALPSTQLINGYGPTESTTFAATWRIP
                     RDFAAANRSVPIGLAIPDTTIYILNRRLEPIPDGFIGELFIGGRGLARGYRNRPELNA
                     EKFVADPFAGHGARLYRTGDLVRRLPGGAIDFIGRGDGQVKIRGYRIETGEIEACLRE
                     HAGVRSGAVVVREDRPGDKRLVAYVVADDS"
BASE COUNT          344 a          803 c          690 g          325 t
ORIGIN      
        1 gatcaagtgg aaggacgaat tcaaacagcg cgcaaccctg ttccacctgc agcccggcca
       61 gggcgcatac atgccgtcga ccagtccgca catggtggag aacgacgaca acccctcgat
      121 cacggtgagt ttcacgtact acaccgacgc gacgcgccgt aacagccggc tgcatggact
      181 gcaccagcgc atgcgcaacg ccggcatgtc gcccgccccg gtgggcacac gcgccgcgcg
      241 cgacgccgtg ctcgggtccc tgacggcggt cagcaccggc gtacgcgata cggtcgcgcg
      301 ccttcgcagc agcaacgcgc gtgtggacac cgcgcgttac gcgcacgcac aggtcgggtg
      361 aaagcgtgcg ggacatgagt cacccgcaaa gctccgcgcg gcgcacgctc gccgacatct
      421 gcgcggtgca ggccgattcc gcgggcgtcg cattccacga agtcgcgctc gacgcgccga
      481 tcgccgacac gctgcgcaac gagaaagata cgcgggcggc attggtgcgc gcgtggaccg
      541 ccacggtcgc cgcgctggtc ggcgaagaac cgggcgacag cggcgtgtgg tgcttcgatg
      601 gcgccgccgc ccccgaggcg atagtcgtct ggcgcgcgac gatcaaaccc acgggaccca
      661 tcacgctgcg cgtcgaatcc acgcgcctcg acaagaacac gctgagcgcg ctcgccggat
      721 tgctgatgcg tgcgcttgcg cagcccgact cgttcacgct gctcgacgcc tccgcgcgcg
      781 aaaccgtttt gaacggccac catgcgccgc tgcgcgcgat cgcccctggc gataccgtgc
      841 acgcgctgtt ttccgcgcag gcgcaccgca cgcccaatgc accggccgtc gaagccggcg
      901 acgtcacgct cagttatgcg gacctcgacg gccgctcggg tgcctgggcc gcggccctcc
      961 gcgagcgcgg tgtatcggcc ggcgccacag ttgcggtgct catgcagcgc tccgccgacg
     1021 ccatcgtcgc cctgctcgcc atcctcaagg ccggcgccac ctatctaccg ctcgaccagg
     1081 cgtatccgcc ggcgcgcctg cgtttcatgc tcgaggacag cggcgcgacg ctgctgctca
     1141 ccgacgcgac gcccggcgac atcctgccga ccggctgccg cgcactggcc gttcaggacc
     1201 tcgccggcgc gcgtggcagc ttcagtgatg cgggcaacgt cagcgacgtc gcctacgtca
     1261 tgtacacctc cggctcgacc ggaactccga aaggcgcgga gattccgcac gccgccatcg
     1321 tgcggctggt gcgcgagccg ggttacatca cactcgatac cgccaccgtt ttcctgcacg
     1381 ccgctcctct cggcttcgac gcttcgacgc tcgagatctg gggcccgctg ctgaatggcg
     1441 gccgctgcgt ggtgcatgac gaaattctgc ccaccggcgc cggcctcgcc gccacgatcg
     1501 cgcggcatcg cgtcacctcc gcgtggctca ccgcggcgct gttcaatgcc atcgtcgatg
     1561 aaaaccccgc gcaactcgcc ggcatcgccg aactgctgac cggtggcgag gcgttgtcgg
     1621 tcgcgcacgt gcggcgcgca tacgccgcgc ttccatcgac gcaactcatc aatggctacg
     1681 gcccgacgga gtcgacgacg ttcgccgcga cgtggcgcat tccgcgggac ttcgccgcag
     1741 ccaaccgctc ggtgcccatc ggcctcgcga ttcccgacac cactatctac attctcaacc
     1801 gccgcctgga accgattccc gatggtttca tcggcgagct gttcatcggc ggccgcggac
     1861 tcgcgcgcgg ctatcgcaat cgccctgaac tcaacgccga aaagttcgtc gccgacccgt
     1921 tcgcgggcca cggcgcgcgg ctctatcgca ccggtgatct cgtgcgccgg ctgcccggcg
     1981 gcgccatcga tttcatcggc cgcggcgacg gccaggtgaa gatccgcggc taccgcatcg
     2041 aaaccggcga gatcgaagct tgtttgcgcg aacacgccgg cgtgcgaagc ggcgcggtgg
     2101 tggtgcgcga agaccgcccg ggcgacaaac gcctcgtggc ctatgtcgtg gccgacgact
     2161 cg
//
LOCUS       DATDEU010000291         3553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1803156, whole genome shotgun sequence.
ACCESSION   DATDEU010000291 DATDEU010000000
VERSION     DATDEU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803156"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..2194
                     /locus_tag="VIV63_07035"
     CDS             209..2194
                     /locus_tag="VIV63_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6124388.1"
                     /translation="MGASSFKSKRPMAVIATPDLDLWAQLGPMLESTCQLRHADSLSS
                     AANMLKAGTRTILVADLRGMNPEDFAPITASPHNPVVIAIRDAASAGTVDALMLEGAI
                     QALVDSPVESGPMLRAMNDAARISSTADALSAVTAPSGGSAGGEEKSGKSPVMMIGVV
                     VAVLAAAAGGWFFMSKGSGDAPAAADSGAAPSASSAAPAAGKSPAAGSTAPAASLDEV
                     LEKARLAMDERRYIEPAKNNALEHFRTVLQLEPGNAEATEGLKRLGGLLIARARTALD
                     ERRFDNALAELEAARSIDPNDARLVEVDARLDSMRAQTALAQIQATLAAQNFDRAQTL
                     LEAAQKDALLPPAQIAQLSKDLDSKRRSINISRFVSSAESRLTNGRLVTPAGESAKDA
                     IQSLREAGASAELVTRLNNDLNQRLLASARDAAAKGDEAGMKAMLAAARENGVSAAVI
                     NAANRDITAAAQKTQRTNDDIARFAKAAQDRLASGNLIQPANDSAVSNAERLRLLDVR
                     HPTTVQVVRDVRARLISDARARLTAGRPTDASALLDAAESLGGDSDLGDLRAAITNAQ
                     QAVATAAAAPKLPPLKMARPPRPKYPASAKGVEGWVRVEFYVTPEGRTERVRVLSSEP
                     AGLFDAAAISAMEGARFEEFESTESRLAVQRIVFKPE"
     gene            2231..>3553
                     /locus_tag="VIV63_07040"
     CDS             2231..>3553
                     /locus_tag="VIV63_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124389.1"
                     /translation="MTKKFSSRMQGARAVWVAVGILALSFLVGVLAPAWYFAGRLVEN
                     NTAVRAIGELQQQPSRMQAALNSIQDRLRARGFVRDSIEQLKRATQQFETSTASLKAG
                     GSVSIGGFGSGDDVQKTIDEMNAAWRKYQPALAPVANFTSIPYSDSEREGTQLNMDGR
                     ELQRAVTNAATQARAYTPVLEKNLSQIIAGLQSSSDSLATALRSVVLIGVGLAAALAA
                     LVGYLSLARGKQAAAALAARQQTEDILRTVREGLFLLDADLKIGEIHSDATGSLFQRE
                     SIAGITFEDLLRNLVSAKTLAIATKFVKVLWSERTKENLIRSINPLNEVEVITGGDGK
                     QDKGETRYLEFNFHRVRRDGAITHVLVSVSDVTARVALAAQLKGAQDNAQTQMDAFLS
                     ILHVDPNQLSSFLDDSDVAFKMINATLRDPARDSASFRRKIDGLFRQIH"
BASE COUNT          648 a         1235 c         1119 g          551 t
ORIGIN      
        1 gtatgtgact cagtgccgat caacggcaac gcactgactt aatactggcc ctcactgtcg
       61 ttccccggag acgggccagg aaatggccgg cgccgcgagg aagagccatc gtcgcaaaac
      121 tagttcgatg agaggtcgag ggtccatgca agcacgttca gaattaggcg cgaccgcgca
      181 caatgacacc gcgggaaggg gcaataacat gggtgcaagc tcattcaagt cgaagcgtcc
      241 gatggccgtc atcgccacgc cagacctgga cttgtgggcg cagctcgggc ccatgctcga
      301 atccacctgc cagcttcgcc acgcggacag cctcagctcc gccgccaaca tgctgaaagc
      361 cggaacgcgc accatcctgg tcgccgacct gcgcggcatg aatcccgaag acttcgcacc
      421 gatcaccgcg agccctcaca accctgtcgt catcgcgatt cgcgatgccg cctcggccgg
      481 cacggtcgat gccctgatgc tcgaaggcgc catccaggcg ctggtcgatt cgccggtcga
      541 atccggcccg atgctccgcg cgatgaacga cgcggcgcga atttcctcca cggctgacgc
      601 gctgagcgcg gtcaccgctc cctcgggcgg aagcgccggc ggagaagaga agtccggcaa
      661 gtcgccggtc atgatgatcg gcgtcgtggt cgcggtgctt gccgccgccg cgggtggctg
      721 gttcttcatg tccaagggtt cgggcgatgc cccggccgcc gccgattccg gcgccgcgcc
      781 ttccgcgtcg tccgctgctc cggccgctgg caagtcgccg gccgctgggt cgactgcgcc
      841 ggccgcttcg ctcgacgaag tgctcgagaa agcgcgcctc gcgatggacg aacgccgcta
      901 catcgaacct gccaagaaca acgcgctcga acatttccgc accgtgctgc aactcgagcc
      961 gggcaatgcg gaagccaccg aaggcttgaa gcgcctcggc ggcctgctga tcgcccgcgc
     1021 ccgtaccgcc ctcgacgaac gccgtttcga caacgcgctc gccgaactcg aagccgcgcg
     1081 cagcatcgat ccgaacgatg cgcgcctggt cgaggtcgac gcgcgtctcg actccatgcg
     1141 tgcccagact gcgctcgcgc agatccaggc gacgctcgcc gcgcagaact tcgatcgtgc
     1201 gcagaccctg ctggaagccg cgcagaaaga tgcgctgctg cccccggcgc agatcgcgca
     1261 gctttccaag gatctcgaca gcaagcgccg ctcgatcaac atcagccgct tcgtatccag
     1321 cgcggaatcg cgactgacca acggtcgcct ggtgacgccg gctggcgagt ccgcgaagga
     1381 cgcgatccag agcctgcgtg aagcgggcgc ttcggccgaa ctcgtcacgc gactcaacaa
     1441 cgacctgaat cagcgcctgc tggcttccgc gcgcgacgcg gccgccaagg gcgacgaggc
     1501 gggcatgaaa gccatgcttg ccgcggctcg tgaaaacggc gtctcggccg cggtcatcaa
     1561 cgccgcgaat cgtgacatca ccgctgccgc ccagaagacg cagcgtacga acgacgacat
     1621 cgcgcggttc gccaaggcgg cgcaggatcg cctcgcgagc ggcaacctga ttcagccggc
     1681 caacgattcg gcggtttcga acgccgaacg gctgcgtctg ctcgacgtgc gccatccgac
     1741 gacggtgcag gtggtgcgtg acgtgcgtgc ccgcctcatc agcgacgccc gcgcccgtct
     1801 gaccgcgggc cggccgaccg acgcttccgc gctgctcgat gcggccgaga gcttgggcgg
     1861 cgactccgat ctcggcgatc tgcgcgccgc gatcaccaat gcccagcagg cggttgccac
     1921 cgcggctgcg gcccccaaac taccgccact caagatggcg cgtccgccgc ggccgaaata
     1981 tcccgcgtcc gccaagggcg tggaaggctg ggtgcgcgtc gagttctacg taacaccgga
     2041 aggccgcacc gagcgtgtgc gcgtgttgtc gagcgagccc gcgggcctgt tcgacgcagc
     2101 ggccatctcg gcaatggaag gtgcccggtt tgaagaattc gagtcgactg aatcgcgtct
     2161 cgcggttcag cgcatcgtgt tcaagccgga gtgatggagg gtgagctctg tgagtaaaga
     2221 taaagcaaaa gtgaccaaga aattttcgtc acgcatgcag ggcgctcgcg ccgtgtgggt
     2281 ggcggtaggt atcctggccc tgtcattcct ggtcggcgtt ctcgcgcccg cctggtactt
     2341 cgccggtcgc ctggtggaga acaacactgc ggtccgcgcc atcggcgaac tgcagcagca
     2401 gccgtcgcgc atgcaggcgg cgctcaactc catccaggat cgactgcgag cccgcggctt
     2461 cgtgcgcgac tccatcgaac agttgaagcg cgccacgcag cagttcgaga cgtccactgc
     2521 ctcgctcaag gcgggcggca gcgtcagcat cggcggcttc ggcagcggcg atgacgtgca
     2581 gaagaccatc gacgagatga acgcggcctg gaggaagtac cagcccgcgc tcgccccggt
     2641 cgccaacttc acctcgatcc cgtactcgga cagcgagcgt gaaggcacgc agctcaacat
     2701 ggacggccgc gaactccagc gcgccgtgac caacgccgcc acccaggcgc gcgcttatac
     2761 cccggtactc gagaagaacc tctcgcagat catcgccggc ctgcaaagct cctccgattc
     2821 gctggcgacc gcgctgcgtt cggtggtgct catcggcgta ggcctcgcgg ccgcactggc
     2881 cgcgctcgtc ggttacctct cgctggcgcg cggcaaacag gccgcggccg cactggccgc
     2941 ccgtcagcag acggaagaca tcctgcgcac cgtgcgcgaa ggtctcttcc tgctcgacgc
     3001 cgacctcaag atcggtgaga ttcactccga cgcgaccggc tcgctgttcc agcgcgagtc
     3061 catcgccggc atcactttcg aagacctgct gcgcaacctg gtgtccgcga agacgctggc
     3121 cattgccacc aagttcgtca aagtgctgtg gagcgagcgc accaaggaaa acctgatccg
     3181 ctcgatcaat ccgctcaacg aagtggaagt gatcaccggc ggcgacggca aacaggacaa
     3241 gggcgagacc cgctacctgg aattcaactt ccaccgcgtt cgccgcgacg gcgccatcac
     3301 ccacgtgctg gtgtcggtat ccgacgtgac cgcgcgcgtg gccctggctg cgcagttgaa
     3361 gggcgctcag gacaacgcgc agacgcagat ggacgcgttc ctctcgatcc tgcacgtcga
     3421 tccgaaccag ttgagctcgt tcctcgacga ctcggacgtc gcgttcaaga tgatcaacgc
     3481 cacgctgcgc gacccggctc gcgacagcgc ttcgttccgc cgcaagatcg acgggctgtt
     3541 ccgccagatc cac
//
LOCUS       DATDEU010000292         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1803307, whole genome shotgun sequence.
ACCESSION   DATDEU010000292 DATDEU010000000
VERSION     DATDEU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803307"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1956
                     /locus_tag="VIV63_07045"
     CDS             <1..1956
                     /locus_tag="VIV63_07045"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124390.1"
                     /translation="RDGLHLYTTNPDGTDTQLHYGAVSHMTGTQVAGVNTAVEFMRAR
                     EMSDGRLMVLTRQRTNVDFGGNLTIIDANRFVENTQTTLAAAGSPGPAQTPATLNDVR
                     TVPGPSPGGRFTSGFPLWDGSGRILVSWTQCRLLDDVDPTRIVPCTTDRLAAPNVQTA
                     PPLYSIWMFTPSQNTILPIMPPVEGVMVTEAVAAQPRTPLPAVIVDKQPPFLDADLVA
                     EGTGLLSIRSVYDIMGSTGTVSIPSISNPTNPGPYNARLVRFIRIEKPVSIPDDDDSA
                     DPDNAAFGPTGVMREIVAYAPVEPDGSVRMKVPANIAFQISLLDANGRRVNNVNGSDN
                     RHRSWLTVRPGEVLECNGCHTRNNATPMVHGRKDLFTAAYNGGSAGPFPGTVTSISAE
                     SGDTMARARSRPTSSCPDSTLAERPAACGLQSITPSADVVFEEIWAPGALPATSFAWS
                     HQDLATARPTNASCYPVWTPTCRITIHYQTIGARPGHIMPLWTVDRVPADPLGIDTNG
                     DGILEPTYKCTSCHDRVSVVAAVQLPAASLELLPGTACNAQNLYDNTYCQLLRPRDEL
                     VLDVGGNVVTAQSLIPLDANGQPTSIVPLITAPMTATNARGSRFFNVMNTAPHQGMLT
                     PAELRLLSEWLDIGAQYYNDPFPPTPE"
     gene            2126..>2658
                     /locus_tag="VIV63_07050"
     CDS             2126..>2658
                     /locus_tag="VIV63_07050"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124391.1"
                     /translation="MFHVVAREQSVDVLHRRTDWFKVRDESGVEGWARARDMRRTKLA
                     DGSPFVFNLGDRAGFTTHDWEIGIGGGDYGGANLIAVMGAYSLTDNMKVDLSVSQFLG
                     NASNGWKAELGLQHVLFPEWRFSPFLTLGTGYVETKPRATIVLPLDRADQTAYAGAGA
                     RFYLTRRFFLRADYRWHT"
BASE COUNT          467 a          924 c          800 g          467 t
ORIGIN      
        1 cgcgacggcc tgcacctgta caccacgaac cccgacggca ccgacacgca gctgcattac
       61 ggcgcggttt cgcacatgac cggcacgcag gtcgcgggcg tcaacacggc ggtcgaattc
      121 atgcgcgcgc gcgagatgag cgacggccgc ctcatggtgc tcacgcgcca gcgcaccaac
      181 gtcgacttcg gcggcaatct cacgatcatc gacgcgaacc gcttcgtcga gaacacgcag
      241 accacgctcg ccgccgcagg ctcgccgggt cccgcgcaga ctcccgcgac gttgaatgac
      301 gtgcgcaccg ttcccggccc gtcgccgggc ggccgcttca cttcgggctt cccgctgtgg
      361 gacggctcgg gtcgcatcct cgtgagctgg acgcagtgcc gcctgctcga tgacgtcgac
      421 ccgacgcgca tcgtgccctg caccaccgac cgtctcgccg cgccgaacgt gcagacggcg
      481 ccgccgctgt acagcatctg gatgttcacg ccttcgcaga acaccattct tcccatcatg
      541 ccgcccgtcg aaggcgtcat ggtcaccgag gccgtggccg cgcagccgcg cactcccctg
      601 ccggccgtga tcgtggacaa acaacctccg ttcctcgatg ccgacctcgt cgccgaaggc
      661 acgggtctgc tctcgatcag gagcgtctac gacatcatgg gctccacggg aaccgtgagc
      721 attccgagca tctcgaaccc gacgaatccc ggcccgtaca acgcgcgtct ggtccgcttc
      781 atccgcatcg agaaaccggt atcgatcccg gacgatgacg acagcgccga tcctgacaac
      841 gccgcattcg gcccgaccgg cgtcatgcgc gagatcgttg cctatgcacc cgtcgaaccc
      901 gatggttcgg tgcgcatgaa agtgcccgcc aacatcgcgt tccagatcag cctgctcgat
      961 gcgaacggtc gtcgtgtgaa caacgtgaac ggctcggaca accggcatcg ctcatggctc
     1021 accgtgcgtc cgggcgaagt gctcgagtgc aacggctgcc atacgcggaa caacgcgacg
     1081 ccgatggttc acggccgcaa ggacctgttc acggccgctt acaacggcgg aagcgctgga
     1141 cctttcccgg gaacggtgac ctccatttcc gccgagagcg gcgacaccat ggcgcgcgcg
     1201 cgttcgcgcc ccacgtcgtc gtgcccggat tcgacgctcg ccgaacgccc tgcggcgtgc
     1261 ggtctgcaat ccatcacgcc gagcgcggat gtcgttttcg aagaaatctg ggctccgggc
     1321 gcattgcctg cgacgtcctt tgcctggagt caccaggatc tcgcgaccgc gcgaccgacc
     1381 aacgcgagtt gttatccggt ctggacgccg acctgccgca tcaccatcca ctaccagacg
     1441 atcggcgccc gtcccggaca catcatgccg ttgtggacgg tggatcgcgt tccggccgac
     1501 ccgctgggca tcgacacgaa cggcgatgga attctcgaac ccacttataa gtgcacgagc
     1561 tgccacgacc gtgtgagcgt ggtcgcagcc gtgcaattgc cggctgcgtc cctcgagttg
     1621 ttgccaggca ccgcatgcaa tgcccagaac ctctacgaca acacgtactg ccagttgttg
     1681 aggccgcgcg acgaactcgt tctggatgtc ggcggcaacg tggtcacggc acagagcctc
     1741 atcccgctgg acgcgaatgg ccagcccacc tcgatcgttc ccctgatcac ggcaccgatg
     1801 accgcgacca atgctcgggg ctcgaggttc tttaacgtaa tgaacacggc gccgcatcaa
     1861 ggtatgctca cgcccgctga actgcgcttg ctttcggagt ggctggatat tggagcccag
     1921 tattacaacg acccgttccc gccgactcct gagtaacctc gcgttcgcca ccgtcttcgc
     1981 gtgtcttctt tcggtcccgt cgttagcgaa acagaccgag gaggaaaagg ccgcaaaggc
     2041 agcagcggcc caggagaagc tgctacaggt cttcgtcagc gagccgtacc tcgagcttgc
     2101 gaccgggccc ggccgcggct atcccgtgtt tcacgtggtg gcgcgcgagc agtccgtcga
     2161 cgtgctccat cgccgcacgg actggttcaa ggttcgcgac gaatcgggag tcgaaggctg
     2221 ggcgcgcgcg cgcgacatgc gccgcacgaa gctcgccgac gggtcgccgt tcgtcttcaa
     2281 tctcggcgac cgcgccggct tcacgacgca cgattgggaa atcggcatcg gtggcggcga
     2341 ctacggcggt gcgaacctca tcgcggtgat gggcgcttat tcgctcaccg acaacatgaa
     2401 ggtcgacctg tcggtctcgc agttcctcgg caatgcgtcg aatggctgga aagccgagct
     2461 cggcttgcag catgttttgt ttcccgaatg gcggttctcg ccgttcctga cgctgggcac
     2521 cggctatgtg gagaccaagc cccgcgccac gatcgtgctg ccgctcgatc gcgctgatca
     2581 gaccgcctac gcaggagcgg gggcacgttt ttatctcacc cggcgctttt tcttgcgcgc
     2641 cgactatcgc tggcacac
//
LOCUS       DATDEU010000293         3619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1803355, whole genome shotgun sequence.
ACCESSION   DATDEU010000293 DATDEU010000000
VERSION     DATDEU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803355"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2076)
                     /locus_tag="VIV63_07055"
     CDS             complement(<1..2076)
                     /locus_tag="VIV63_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY6124392.1"
                     /translation="MDHVYPPGPATVPPNLTAATTAYKHRAWLAMAGLAAFIALYFAL
                     SGWFAWTAWRLFDGMFGQGGHFDLWQFVAAVCSTFLAVFMLKALFFIQHRFDVEDIEI
                     TRQDQPQLFDFIDRLADEARAPRAHKVYLSARVNAAVFYDLSLLNLIVPSKKNLEIGL
                     GLVNVVTLGELKAVLAHEFGHFAQRSMAVGRWVYISQQIAGHVVARRDALDKLLQQLS
                     RFDLRIAWVGWILSVIVWSIRSMMDVLFRGVLLAQRALSRQMEFQADLVAVSLTGSDA
                     LIHALHKLNAADDAWDKALSFANSECRAKRGITDLFAVQNRIIERMREILNQPLYGQV
                     PPLPATARDSHRVFKTELAQPPRMWGTHPPSADRENNAKQRYVAAPVDERSAWEVFRN
                     PQGLKEQMSAHVFREATVEPTAMEKTFQHLEKQYGRAFLNRAYRGVYLHRSPVRYAGS
                     VDALYGAETPPANLARALDSLYPEDLANKVELLNEKLEEKHALEALRDQVAQAPGGII
                     RHNGAEIRRTELPRAIASLEREIMVVRGQLETHDKLCRSAHLSAARVLEKGWPEYLRS
                     LAAMLHYADHTEANLRDVQGFVANIYGVVTADGRVSSKELNRLLEGCQQLYAVLSTVH
                     GAAKEVTLDRTLLRRLEVESWDALLEEFKLPPPNRENIGQWLNVIDGWTGAAIAAAAR
                     LRDASLEQLL"
     gene            2282..3592
                     /locus_tag="VIV63_07060"
     CDS             2282..3592
                     /locus_tag="VIV63_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6124393.1"
                     /translation="MTLERAGWMCGACSSGYPVIGEIPWLFPEPRQALAEWRGRLGLL
                     TQHLASEAKAMRASLADSALDPGTRRRLEHVARAHDEQISRLRQILAPLGLDQATVAE
                     ATHRGLGTRLPTEQGLTNYYVNLHRDWSWGDEENQAAIGELTALLGGSVAGLGRVLVQ
                     GAGAGRLAYDLHAASDATLTVATDFNPLLLFAARELFAGRTVELYEFPIAPRSIEDHA
                     VLRRLAAPAAARPGLELVAADALQPPFADGAFDTVVTPWFIDIIGEPFARVAARVNLL
                     LKPGGRWINYGSLAFSRAAHAERLSLEEVRGLMPKAGFAGFEMREASIPYMRSPASRH
                     ARIESTISWVANKVGPPSETPRARALPDWLLQPDKPIPRTPALEMQAISSRVHAFLLA
                     LINGERSMRDMARVLVEQRLMSAQEAEEQVRLFLARLHEESESQ"
BASE COUNT          597 a         1137 c         1250 g          635 t
ORIGIN      
        1 cagcaactgc tcgagcgacg catcacgcag ccgggcggcc gcggcgatcg cggcgccggt
       61 ccagccgtcg atgacgttga gccactggcc gatgttctcg cggttgggcg gcggtagttt
      121 gaactcctcg agcagcgcat cccagctctc gacttccagg cgacgcagca gcgtgcgatc
      181 gagcgtgacc tctttcgcgg ctccgtggac ggtgctgagc accgcgtaga gctgctgaca
      241 accttcgagc aaccggttga gctccttgga gctcacgcgg ccgtccgcgg tgaccacgcc
      301 gtagatgttg gccacgaagc cctgcacgtc gcgcaggttc gcttcggtgt ggtcggcgta
      361 atgcagcatg gccgcgaggg agcgcaggta ttccggccat cccttctcga gaaccctggc
      421 ggctgacaga tgcgcgctgc ggcagagctt gtcgtgggtt tcgagttgac cgcgcacaac
      481 catgatctcg cgctcgagac tggcgatggc gcgtggcaat tcggtgcggc ggatctccgc
      541 gccgttgtga cggatgatgc cgcccggcgc ctgcgcgacc tggtcgcgta acgcttcgag
      601 cgcgtgcttt tcctcgagct tctcgttgag cagctcgacc ttgtttgcga gatcttccgg
      661 atacagcgag tcgagcgcgc gagccagatt ggccgggggc gtttccgcgc catacagggc
      721 atcgacggag ccggcatatc gcaccggcga ccgatgcaga tagacgccgc ggtatgcgcg
      781 gttcaggaac gcgcggccgt attgcttctc gaggtgctgg aaagtctttt ccatggccgt
      841 cggctcgacg gtggcctcgc ggaaaacgtg tgccgacatc tgctccttca atccttgcgg
      901 attgcggaac acttcccacg cgctgcgctc gtccacgggc gccgcgacgt aacgttgttt
      961 ggcgttgttc tcgcggtcgg cgctgggtgg atgcgtgccc cacatgcgcg gcggctgcgc
     1021 cagttccgtc ttgaacacgc gatgcgaatc gcgcgcggtg gccggaagcg gcggcacctg
     1081 gccatacagc ggctggttca ggatctcgcg catgcgttcg atgatccggt tctgcacggc
     1141 gaacagatcg gtgatgccgc gtttcgcgcg gcactcggaa ttcgcgaatg acagcgcctt
     1201 gtcccaggcg tcgtcggcgg cgttgagctt gtgcaacgcg tggatcagcg cgtcgctgcc
     1261 cgtgagcgac acggcgacga ggtccgcctg gaactccatc tggcgtgaca acgcgcgctg
     1321 cgccagcagc acgccgcgga acagcacgtc catcatcgag cggatcgacc agacgatgac
     1381 ggagaggatc cagcctaccc aggcgatgcg caggtcgaaa cgcgagagct gctgcagcag
     1441 cttgtcgagc gcatcgcggc gcgccacgac atggccggcg atctgctgcg agatgtagac
     1501 ccagcggccg accgccatgg agcgctgcgc gaaatgtccg aactcgtgcg caagcacggc
     1561 cttgagctcg ccaagcgtga ccacgttgac caggcccagt ccgatctcga ggtttttctt
     1621 cgaggggacg atgaggttca acaacgacag gtcgtagaac accgccgcgt tcacgcgcgc
     1681 cgagaggtac accttgtgag cgcgcggcgc gcgggcttcg tccgccagcc ggtctatgaa
     1741 gtcgaagagt tgtggctggt cctggcgcgt gatttcgatg tcttcgacat cgaaacgatg
     1801 ctggatgaag aacagcgcct tgagcatgaa cactgcgagg aaggtggagc agaccgcggc
     1861 gacgaactgc cagaggtcga aatgcccgcc ctggccgaac atgccgtcga acagtcgcca
     1921 ggcggtccag gcgaaccagc ccgacagtgc gaaatacagg gcgatgaagg cggcgagacc
     1981 cgccatggcg agccacgcgc ggtgtttgta ggcagtggtg gcggccgtga ggttgggcgg
     2041 cacggtcgcc gggcccggtg ggtagacgtg atccatccag aactccctcg atatatgcgg
     2101 ctcagtctga gtgagcggtg cgggttcgtg aaggaatctg tcacgcgaat gccaactcgg
     2161 acacgctgcc gggtgcggcg ggaattgacc tccggccagc cgacggccag cattgccgcg
     2221 cttgtcacaa acacctacct tgtcgacctt gtttgcctgc ccccggtgcc atcgaggccc
     2281 ggtgacactg gagcgcgcgg gctggatgtg tggcgcctgc agcagtgggt atccggtcat
     2341 cggcgaaatt ccgtggctgt tcccggaacc gcgccaggcg ctggcggaat ggcgggggcg
     2401 tctgggtctg ctgacgcagc acctggcgtc cgaagccaag gcgatgcgcg cgagtctcgc
     2461 ggacagcgcg ctggatcccg gcactcgtcg aaggctcgag cacgtggcgc gggcgcatga
     2521 cgagcagatc tcgcgattgc ggcagatcct ggcgccgctc gggctggacc aggccactgt
     2581 cgccgaggcc actcaccgcg ggctgggcac acgtctgccc accgagcagg gtctcactaa
     2641 ctactatgtg aacctgcacc gcgactggtc gtggggcgat gaagagaacc aggccgcgat
     2701 cggcgaactg acggcgctgc tcggcggtag cgtggccggt ctcgggcgcg tgctggtgca
     2761 aggcgcgggc gcagggcgcc tggcctacga tctgcacgcc gcgtccgatg ccacgctgac
     2821 cgtggccacg gatttcaatc ccctgttgtt gttcgcggcg cgcgagttgt ttgccggacg
     2881 gaccgtcgag ctctacgaat ttccgattgc cccgcgcagc atcgaagatc acgccgtgtt
     2941 gcgccggctt gccgcgcccg cggccgcgcg gccgggcctc gaactcgttg ccgcggacgc
     3001 gttgcagccc ccattcgccg acggtgcttt cgataccgtg gtcacgccgt ggttcatcga
     3061 catcatcggc gagccgttcg cgcgcgtggc ggcgcgcgtg aacctgctgc tcaaacccgg
     3121 cggccgctgg atcaactacg gttcgctggc gttttcacgc gccgcgcacg cggaaagact
     3181 gagcctcgaa gaagtgcgcg ggctcatgcc gaaggcgggc ttcgcgggtt tcgagatgcg
     3241 cgaggcgtcg attccgtaca tgcgatcgcc ggccagccgg cacgcgcgta tcgagagcac
     3301 gatcagctgg gtggctaaca aagtggggcc gccgtcggaa accccgcgcg cgcgcgcgct
     3361 tccggattgg ctgctgcagc ctgacaagcc cataccgcgt acgccggctc tcgagatgca
     3421 ggcgatctcc agccgcgtgc atgcgttcct gctggcgctg atcaatggcg agcgttcgat
     3481 gcgcgacatg gcgcgggtgc tcgtggagca gcgcctgatg tccgcgcaag aggccgaaga
     3541 gcaagtgcgc ttgtttctcg cccgcctgca cgaagagagc gagtcgcagt aatgggggta
     3601 atggggacat tcctgattt
//
LOCUS       DATDEU010000294         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_52924, whole genome shotgun sequence.
ACCESSION   DATDEU010000294 DATDEU010000000
VERSION     DATDEU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52924"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..440)
                     /locus_tag="VIV63_07065"
     CDS             complement(99..440)
                     /locus_tag="VIV63_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY6124394.1"
                     /translation="MGRKLYVGNLPYQTGETELTELFSRAGTVESARIMRDMATGRAR
                     GFGFVEMASDEEAQRAISELNEFKMEGRPLVVNEARPKPMGGGGGGGGGFERGNRGGG
                     GGGGGRREPRW"
     gene            complement(715..981)
                     /locus_tag="VIV63_07070"
     CDS             complement(715..981)
                     /locus_tag="VIV63_07070"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY6124395.1"
                     /translation="MARTVFCAKLQKDLPGLDEAPWPGELGTRIYDNISVQAWKMWED
                     RMKMILNEYRLMPWQKEAQQLMAKQMEEFLFSGTDALPPEYKPK"
     gene            1028..>2158
                     /locus_tag="VIV63_07075"
     CDS             1028..>2158
                     /locus_tag="VIV63_07075"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY6124396.1"
                     /translation="MSVSSTVERGRQGNFEDNVNAYFNHAATFCNYAPGLLEQIKVCN
                     SVYAFAFPVRHADGSIEVVHAWRAEHSHHKLPTKGGVRYSPQVDESEVKALAALMTYK
                     CALVDVPFGGAKGAVQIDPARYTVEQLERITRRYTHELDRKQFIGPGIDVPAPDYGTG
                     EREMSWIADTYSQLHTGELEALACVTGKPVTEGGIYGRREATGRGLMFALAEACRSAD
                     DMRALGLSVGLDGKRLVVQGLGNVGYHAAKFCREQGAIIVALAEREGAIQNPKGLNED
                     EVFNFRKRTGSLLGFPGATDILRSADALELECDVLVPAALENVLTSENAARIKARIIL
                     EGANGPTTPEADDVFRKKGIMVIPDVFANAGGVTVSYFEWLKN"
BASE COUNT          396 a          700 c          654 g          408 t
ORIGIN      
        1 aggtgcaacg ggtgcgaggt gcaacgagtg cgcacctgtt gcagccattg caccggtcgc
       61 acccattgca cctgtcggtc gcacttattg catctgatct accagcgtgg ctcccggcgt
      121 ccgccgccgc caccgccgcc cccgcggttg ccgcgttcga agccgccgcc accgccgcca
      181 ccaccaccca tgggtttggg ccgcgcctcg ttcacgacca gcgggcgtcc ttccatcttg
      241 aattcgttca gctctgagat agcgcgctgt gcttcctcat cgctggccat ctcgacgaat
      301 ccgaatcccc gtgcccggcc cgtggccatg tcgcgcatga tcctcgcgct ttccacggtc
      361 ccggcccgac tgaagagctc ggtgagctcg gtttcgccgg tctgataggg aaggtttccc
      421 acgtacaact tgcgacccat aacgctgact cctcggcccc ctgtgggacc cataaaaaat
      481 cccggaacgg accggacggt ccgtgccgat cgttcacctc gctgagttgt gtgcggagtg
      541 tggagacaga ggctgggggg tcagctcgtt cggcactgcg cagaaaccgt cggtcgggcg
      601 cacgacagct tcagtatacg cctcacaacg ggttggcggt tcgcagttcg cggttggtgt
      661 gaaagctgta ctgaattgcg acgctaccaa cccgccaacc ccaaccacca accactattt
      721 gggtttgtac tccggtggca acgcgtctgt gccgctgaag aggaactcct ccatctgctt
      781 cgccatgagc tgttgggctt ccttctgcca cggcatcaga cggtactcgt tgaggatcat
      841 cttcatgcgg tcctcccaca tcttccacgc ctgcaccgaa atgttgtcgt aaatacgcgt
      901 gcccagttcg ccgggccacg gcgcttcgtc gaggccgggc aggtccttct gcaacttcgc
      961 gcaaaacacg gttcgtgcca tgcggcaatc ctatcagaag ggcagagggc cctgtcataa
     1021 tcaccccatg tctgtttctt ctactgtcga gcgcgggcgt caaggcaatt tcgaagacaa
     1081 cgtcaacgcg tatttcaatc acgcggccac gttctgcaat tacgcgccag ggttgctcga
     1141 gcagatcaag gtgtgcaact cggtgtacgc gtttgcgttt cctgtccggc acgcggacgg
     1201 ctcgatcgaa gtcgtgcacg cgtggcgggc ggagcatagc catcacaagc tgccgaccaa
     1261 agggggcgtc cggtacagcc cgcaggtcga tgagtcagag gtcaaggctc ttgcggcgtt
     1321 gatgacgtac aagtgcgcgc tcgtcgacgt ccctttcggt ggagcgaaag gcgccgttca
     1381 aatcgacccc gcccgttaca cggtagagca gctcgagcgc atcacgcgcc gctatacaca
     1441 cgagctcgat cgcaagcagt tcatcggccc cggcatcgac gtccccgcgc ccgactacgg
     1501 aaccggcgag cgcgagatgt cgtggatcgc ggatacgtac agccagctgc acaccggcga
     1561 gctcgaggcg ctcgcctgcg tcaccggcaa gcccgtcacc gaaggcggca tttacggccg
     1621 ccgtgaagcg accgggcgcg gattgatgtt tgccttggcc gaggcgtgcc gttcggccga
     1681 cgacatgcgc gcgctcggat tatcggtcgg cctcgacgga aagcggctgg tcgtccaggg
     1741 actcggcaac gtcggatatc atgcggccaa gttctgccgc gagcagggcg cgatcatcgt
     1801 cgcgctggcc gagcgcgaag gcgcgattca gaacccgaag ggcttgaacg aagacgaggt
     1861 cttcaatttc cgcaagcgca ccgggtcact ccttggattt cccggcgcaa cggacatcct
     1921 gcgcagcgcc gacgcgctcg agctcgagtg cgacgttctc gtgcctgcgg cgctggagaa
     1981 cgtgttgacc tccgagaacg cggcccgcat caaggccagg atcatcctcg aaggcgcaaa
     2041 cggcccgacg acgcccgaag cagacgacgt gttcaggaag aagggcatca tggtgatccc
     2101 ggatgtcttc gccaacgcgg gcggcgtcac cgtttcgtac ttcgagtggc tgaaaaat
//
LOCUS       DATDEU010000295         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_491573, whole genome shotgun sequence.
ACCESSION   DATDEU010000295 DATDEU010000000
VERSION     DATDEU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491573"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VIV63_07080"
     CDS             <1..426
                     /locus_tag="VIV63_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124397.1"
                     /translation="EAHAFWFEKSLDEAQPFFDGGQSIQRAQELLHMPRPAFRFYVFA
                     FAQFVMSEAAIGDPDAASGFLNNLIAREERDPGSVAQLFARLEPTVDFVAGSQSRFDA
                     SHDIYGDFAEKAAVLKKLMGTTHSPLDPEDQMLDSTDDA"
     gene            426..878
                     /locus_tag="VIV63_07085"
     CDS             426..878
                     /locus_tag="VIV63_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY6124398.1"
                     /translation="MTDTPDYEAMLEVAIAEARRGLDEGGIPIGAALFHNDGRLLGCG
                     HNRRVQEGDPSVHGETDAFRKAGRQRGYRNTIMVTTLAPCWYCSGLAVQFRIGTVVVG
                     ESRTFQGGIDWLRERGVKVVDLQSYECEKMLGDYIAANPEVWNEDIGE"
     gene            complement(893..1294)
                     /locus_tag="VIV63_07090"
     CDS             complement(893..1294)
                     /locus_tag="VIV63_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124399.1"
                     /translation="MRFTSGNSRNQLRERQRRDRATAQVMRVMYPQFASLRLKFDFSE
                     SDPEISALAPHLLVMHPPSQAYFVFPCPYADCDGEFDLTRAVADLARSQEPHASGNQK
                     CGGHRVRDKNGRTPCQLTLEYTLAPAAEPAA"
     gene            complement(1291..1467)
                     /locus_tag="VIV63_07095"
     CDS             complement(1291..1467)
                     /locus_tag="VIV63_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124400.1"
                     /translation="MAKTNYNQAKRQKENARKARQQEKLAKKLARVAGEPETTGTETA
                     EAVPEVATEPKAAP"
     gene            complement(1454..1843)
                     /locus_tag="VIV63_07100"
     CDS             complement(1454..1843)
                     /locus_tag="VIV63_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124401.1"
                     /translation="MLRLNFRSIKGDAPTLTVATFFRFHADGTLRGPENYLVARCVDG
                     CWQLSGRMHRELDCEGPVKLRFSMGANDVPVMLGPFAHVRTAAGMLYGDDSCLNIFVP
                     GRNPGTSAACHELTLLSANGDRPLGKD"
     gene            complement(1862..2143)
                     /locus_tag="VIV63_07105"
     CDS             complement(1862..2143)
                     /locus_tag="VIV63_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY6124402.1"
                     /translation="MKLYVGNLPFSATEDSVRALFAEHGNVEKVALVNDRDTGQPRGF
                     GFVEMANADAQKAMQTLNGRDFGGRPLKVNEAQDKPRGGGGGGGGYNRR"
     gene            complement(2414..>2635)
                     /locus_tag="VIV63_07110"
     CDS             complement(2414..>2635)
                     /locus_tag="VIV63_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124403.1"
                     /translation="RKQVAFRFRTPMDWVDKLRTAYLPVAGIFATLDAGGKRSLRSEL
                     LELVTGFNRATDGTMVVDATYLEVSIKRR"
BASE COUNT          478 a          838 c          832 g          487 t
ORIGIN      
        1 gaggcgcatg cgttctggtt cgaaaagtcg ctggatgagg ctcaaccctt tttcgacggt
       61 ggtcaatcca ttcagcgtgc gcaggaattg ctgcacatgc cccggcccgc gtttcggttc
      121 tacgtcttcg cgttcgcgca gttcgtgatg tcggaggccg ccatcggcga cccggacgca
      181 gccagcggct tcctgaacaa cctgatcgcg cgcgaggagc gtgatcctgg cagcgtcgcc
      241 cagctcttcg cgcgtctcga accgacggtc gatttcgtgg ccgggagcca gtcgcgcttc
      301 gacgcgagcc acgacatcta cggcgacttc gccgaaaagg ccgcagtgct caagaagctg
      361 atgggaacca cacattcgcc actcgatccc gaggatcaaa tgctcgactc cacggacgac
      421 gcctgatgac tgacacacct gactacgaag ccatgctcga ggtcgccatc gccgaagcga
      481 ggcgcggtct cgacgaaggc ggcatcccca tcggcgccgc gttgttccac aacgacggca
      541 ggttgttagg ctgcggccac aaccggcgcg tgcaggaagg cgacccgtcg gtgcacggcg
      601 agaccgacgc attccgcaag gccgggcggc aacgcggtta ccgcaacacc atcatggtca
      661 cgacgctcgc gccctgctgg tactgcagcg gccttgcggt gcagttccgg atcggcacgg
      721 tggtcgtggg tgagtcgcgc acgttccagg gtggcatcga ctggctgcgc gagcgcggcg
      781 tgaaggtcgt cgacctccaa tcgtacgagt gcgaaaagat gctgggcgac tacatcgccg
      841 ccaatcccga agtgtggaac gaagacatcg gcgagtgacc gccggcgttt cgtcaggcag
      901 cgggttccgc cgcgggcgca agcgtgtatt cgagcgtcaa ctggcacggc gtgcggccgt
      961 tcttgtcgcg cacccgatgg ccaccgcact tctggtttcc cgacgcatgc ggctcctgcg
     1021 agcgagcgag gtccgccacc gcgcgcgtga gatcgaactc accgtcgcaa tcggcatagg
     1081 ggcacggaaa aacgaaatac gcctgtgaag gcggatgcat gaccagcagg tgcggcgcca
     1141 gcgcggaaat ttccgggtcc gattcgctga aatcgaattt cagccgcaac gacgcgaact
     1201 gcggatacat cacccgcatg acctgcgccg tggcgcggtc gcgtcgctgg cgctcacgca
     1261 actggttgcg actgttgccg ctggtgaatc tcacggcgcc gccttcggct ccgtcgcgac
     1321 ctcgggaacc gcttcggcgg tctcggtacc ggtcgtttcg ggttcgcccg ctacgcgggc
     1381 cagcttcttt gccagttttt cctgctgacg ggccttgcgc gcgttctctt tctgacgctt
     1441 cgcctggttg tagttagtct ttgccaagag ggcggtctcc gttcgcggaa agcagcgtca
     1501 attcgtgaca ggccgcggat gtgccgggat ttctcccggg cacgaagatg ttgagacagg
     1561 agtcgtctcc ataaagcatg cccgcggccg tgcgcacgtg cgcgaacggt ccgagcatga
     1621 cgggcacgtc gttcgcgccc atggaaaacc gcaacttcac gggaccttcg cagtcgagct
     1681 cgcggtgcat ccgcccgctc agctgccagc atccgtccac gcagcgcgcc accagataat
     1741 tctccggacc tcgcaacgtt ccgtccgcgt gaaagcggaa gaacgtggca acggtcagag
     1801 tgggcgcgtc acctttgatg gatcggaagt tcagtcgcag catgtagttg cggtttacct
     1861 atcagcgccg gttgtacccg ccgccgccac caccaccgcc gcgcggcttg tcctgcgctt
     1921 cgttcacctt gagcgggcgg ccaccgaagt cgcggccgtt caacgtctgc atggcctttt
     1981 gcgcatccgc attcgccatt tcgacgaagc cgaatccacg cggttgaccg gtatcgcggt
     2041 cattgacgag tgcaactttt tcgacattgc cgtgctcggc gaataacgcg cgcacggagt
     2101 cttcggtcgc ggagaaaggc agattaccaa cgtaaagttt catggatgaa tcccaatgag
     2161 cagtggacct acggaaaatc ccgtcggtcg gttcgcgaat gctttgaggt gactgactct
     2221 ggccggaaag cgtctagcga cgcgggtcca gagacggaca agacgaagga tcacaagccg
     2281 ggtctcaagg taccccaaat gagctcgccg tacgggtcta aatggcccta cgtcccgccc
     2341 ttccgcgccg aattattgcg gtgaggcccc ggctgaacgg gacctcgacg tcctgtccgg
     2401 gcgcgccgcc gcatcaacgg cgcttgatcg agacctcgag gtaggtcgca tcgacgacca
     2461 tggtcccgtc agtcgcgcgg ttgaatcccg tcaccagctc cagcagctcg gagcgaaggc
     2521 ttcgtttgcc gccggcatcc agcgtcgcga agattcccgc gacgggaaga taggcggtgc
     2581 gcagcttgtc gacccagtcc atgggcgtgc ggaagcggaa ggccacctgt ttgcg
//
LOCUS       DATDEU010000296         6044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_491673, whole genome shotgun sequence.
ACCESSION   DATDEU010000296 DATDEU010000000
VERSION     DATDEU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491673"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VIV63_07115"
     CDS             <1..416
                     /locus_tag="VIV63_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124404.1"
                     /translation="HCVPPKDVQLALESLFGASVGQIKVIQYSIFVRFHWRAIATTRT
                     NRIYLRGSGKEFFADGPLVLHEYFHVIRQWATGDLTVPRYLLECFRRGYWDNRYEVEA
                     REYTDTEIFRYRALLAQHRTAELPPGVHPIRKKFG"
     gene            complement(423..1607)
                     /locus_tag="VIV63_07120"
     CDS             complement(423..1607)
                     /locus_tag="VIV63_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6124405.1"
                     /translation="MSLRGIWAVFRKEFRENLRDKRTLLSALVFGPVVSPILVAGLVQ
                     FGISHAETQSDESISVAVTHAESAPNLIDYLSARGVTIEKVKFDEAAARNAVATQTHK
                     IILEIPEDFGKRLQEGQPAPIVLYSDSSRAFERRGVGRVRALVSQYGIEIAQLRFLAR
                     GIDPISVLPISVQEIDVSTPTGRSVLVLNMMTYLVLLSMLFGGLYLAIDATAGERERG
                     SLEVLLTTPVPREHLIYGKIGAAASYMLISLVLTVAMFAVAMSFVGLEQLGITTNLGP
                     AAAAKMVACCAPLILFGSAYLTIISAFAKSYREAQTYVQLMITIPTMPLIFAGMMGLQ
                     PKLGLMFVPFLSQHLLMTSVVRAEEILPLHMLVSVVSTLVLGALLAYIAGRLYRREAL
                     LG"
     gene            complement(1604..2347)
                     /locus_tag="VIV63_07125"
     CDS             complement(1604..2347)
                     /locus_tag="VIV63_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6124406.1"
                     /translation="MLEARGLFKSFGKVAAVRGVSFSARDGQITGLLGPNGAGKSTTL
                     RMLYTVLKPDQGDAFINGTSVTEDPLAARRSIGVLSHGAGVYNNLTARENIAYFGELH
                     GLTRADRDARASELIEMLEMQSFADRLAKGFSQGQKLKTALARALVHRPRNLLLDEPT
                     NGLDVMAVRNLRKLLGQLRDAGHCVLFSSHVMQEVEQLCDEVVVINGGQVVAAGTLAD
                     IRARAANSHLEDAFVSLMHGDDAGNGESK"
     gene            complement(2352..3824)
                     /locus_tag="VIV63_07130"
     CDS             complement(2352..3824)
                     /locus_tag="VIV63_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6124407.1"
                     /translation="MMRLCALAGFLGFAAIATATNAVPALPLKPCRLEHPARMLALSA
                     ECGVLKVPENPDKPGGRSIELFVARVPAISLNKKTDPLFLIAGGPGTSAVDLYTSSSG
                     PFERVRRDRDIILVDQRGTGRSHRLDCDYGNDNLFERIDEIEVGAANIKCRDELSKKS
                     DLRMYTTSIAVRDLDKVREALGYQLINLYGNSYGTRVAQHYARRFPKSTRTVILDGVV
                     NPEVVLGPAIAIDAERALERILGRCSADAACAKAFTDPSADYRALRSQLTAKPMKTMV
                     SDAATGRPIHFDFTARHLSAVLRFASYNDDQAALLPLSLHLATHEDNFTPLASQFRVF
                     AYSLQAAFAYGMHNSVACSEDAPLIDEAKLDLVALNATHMGAEQVQQLVEACKDWPKG
                     VVDSDLHAPLKSPAAALLLSGADDPVTPPQYAAIAQKAFADSKHVIVGGHGHGLLSAP
                     CVDRIIANFVTAGSAAGLDATCVDKLKPMPFFITLAGPAP"
     gene            complement(3938..4261)
                     /locus_tag="VIV63_07135"
     CDS             complement(3938..4261)
                     /locus_tag="VIV63_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124408.1"
                     /translation="MNKTTLLAGIGLALSLAMVGTASADTPRYDARQQNQRQRIYNGV
                     GSGELTLRETRRLATGQVHLNRVERRAEADGVVTGRERAHLQHEANQQSRRIYRQKHD
                     RQDRG"
     gene            4385..>6044
                     /gene="mnmG"
                     /locus_tag="VIV63_07140"
     CDS             4385..>6044
                     /gene="mnmG"
                     /locus_tag="VIV63_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY6124409.1"
                     /translation="MTSAGVFDVIIVGGGHAGTEAALAAARMGARTLLLTQSIETLGQ
                     MSCNPAIGGIGKGHLVKEIDALGGVMARGADAAAIQSRTLNASKGPAVRATRVQADRI
                     LYRAFIRRALENQPNLTLFQQECGDLIVDNGVVRGVVTVTGIEFRARSVVLTVGTFLG
                     GRIHVGLDNYSGGRAGDPPSNKLAERLRALPFRTARLKTGTPPRIDGRTIDYSQLATQ
                     FSDDPRPVFSYLGRTSDHPSQLPCHITATTERTHEIIRAATDRSPMFTGKIEGVGPRY
                     CPSVEDKVVRFADKTSHQIFIEPEGLNTHEVYPNGISTSLPYDVQFEFVRTIKGFENA
                     HITRPGYAIEYDFFDPRDLKSSLETKVLAGLFFAGQINGTTGYEEAAAQGLIAGINAA
                     RAAREQDAFIPKRSESYVGVLIDDLVTRGTREPYRMFTSRAEHRLLLREDNADLRLTP
                     AGRELGLVDDARWAFFESKRDGSARELARFETTRIKAADIPAAWSTRVLGDTTTRDAL
                     SAFDLLRRPEVSYDDVVELIGPMNAVDDDRLPAQIRVQLEVAAKY"
BASE COUNT         1107 a         1959 c         1924 g         1054 t
ORIGIN      
        1 accattgtgt cccgccgaaa gacgtgcagc tcgcgctcga gagcctgttc ggcgcgtccg
       61 tgggccagat caaggtcatc cagtacagca tcttcgtgcg ctttcactgg cgcgccatcg
      121 ccaccacccg gacgaaccgc atctatcttc gcggcagcgg caaggaattc ttcgccgacg
      181 gcccgttggt actgcacgaa tactttcacg tcattcggca gtgggccacg ggcgatctga
      241 ccgtgccgcg ctatctgctg gaatgttttc gccgcggcta ctgggacaat cgctacgaag
      301 tggaagcacg cgaatacacg gacaccgaaa tcttccgcta ccgcgcgttg ctcgcgcagc
      361 accgcacggc ggaactgccg ccgggcgttc atccgatccg caagaaattc ggctgagact
      421 tcctagccca gcagggcttc gcggcgatag agccggccgg cgatgtacgc cagcagcgcg
      481 cccaaaacca gtgtgctgac gaccgacacc agcatgtgca gcggcaggat ctcttccgcg
      541 cgcacgacgg aggtcatcag caggtgctga ctcaggaacg gcacgaacat gagtccgagc
      601 ttcggctgca gtcccatcat gccggcgaaa atgagcggca tggtcgggat ggtgatcatc
      661 agctgcacat aggtctgcgc ttcgcgataa ctcttggcga acgccgagat gatcgtcaga
      721 tacgcggagc cgaagagaat cagcggcgcg cagcaggcga ccatcttcgc ggccgcggcc
      781 ggccccaggt tcgtggtgat gccgagctgc tcgaggccga cgaaactcat cgccaccgcg
      841 aacatggcca cggtgagcac cagcgagatc agcatgtacg acgcggcggc gccgatcttg
      901 ccgtagatca gatgctcgcg aggcactggc gtggtcagca gaacctcgag cgatccgcgc
      961 tcgcgctcgc ccgcggtggc gtcaatggca aggtaaagac cgccaaacaa catcgacagc
     1021 aggaccaggt aggtcatcat gttgagcacc agcaccgaac ggccggtggg cgtcgacaca
     1081 tctatctcct gcacggagat gggcaacacc gaaatcgggt cgatgccgcg cgccaggaat
     1141 cgaagctgcg caatctcgat gccgtactgc gacaccagcg cacgcacgcg tcccacgcca
     1201 cggcgctcga aggcgcgcga gctgtcggag tacagaacga tcggtgcggg ctgaccttcc
     1261 tgcaggcgct tcccgaaatc ctcggggatc tccaggatga tcttgtgcgt ctgcgtcgcc
     1321 acggcgttgc gcgcggccgc ctcgtcgaat ttcaccttct cgatggtgac gccgcgggcg
     1381 ctcagatagt cgatgagatt gggcgcactt tccgcatgcg tcaccgccac cgaaatgctt
     1441 tcgtccgatt gcgtctccgc gtgcgaaatg ccgaactgga cgagtcccgc gacgagaatg
     1501 gggctgacca ccgggccgaa caccagtgcc gacagcagcg tgcgtttgtc gcgcaggttc
     1561 tcgcggaact ccttgcgaaa aaccgcccag atgccgcgaa gactcacttc gactcaccgt
     1621 tgccggcgtc gtcgccgtgc atgaggctca cgaaggcgtc ctcgagatgg ctgttggcgg
     1681 cgcgcgcgcg gatgtccgcc agcgttcccg ccgccaccac ctggccaccg ttgatcacca
     1741 ccacctcatc gcacagctgt tcgacttcct gcatgacgtg gctcgagaac aatacgcagt
     1801 gacccgcatc gcgaagctgc cctaacaatt tgcgcaggtt gcgcaccgcc atcacgtcga
     1861 gtccgttggt gggctcatcg agcagcaggt tgcgcgggcg atgcaccagc gcgcgagcca
     1921 gcgccgtctt gagcttctgg ccctgcgaaa agcccttcgc caagcggtcg gcgaacgact
     1981 gcatctccag catctcgatg agctcgctgg cgcgcgcatc gcggtccgcg cgcgtaaggc
     2041 catgcaactc gccgaaatac gcgatgttct cgcgcgcggt gagattgttg tagacgccgg
     2101 caccgtgtga caacacaccg atcgatcggc gcgcggccag cggatcctct gtaacgctgg
     2161 ttccgttgat gaacgcgtcg ccttggtcgg gcttgagcac cgtgtacaac atgcgcagcg
     2221 tcgtcgattt gccggcgccg ttgggcccta acaagccggt gatctgtcca tcgcgcgcgg
     2281 agaagctcac gccgcgcacg gcggccacct tgccgaagga cttgaacaga ccgcgggctt
     2341 ctaacatggc gtcacggcgc aggacccgcc agcgtgatga aaaacggcat gggcttgagc
     2401 ttgtcgacac aggtggcgtc gagtccggcg gccgaacccg ccgtcacgaa attcgcgatg
     2461 atgcgatcga cgcagggagc gctgagcagt ccgtgcccgt ggccgccaac gatcacgtgt
     2521 ttgctgtccg cgaatgcttt ctgcgcgatg gcggcgtatt gcggcggcgt caccggatcg
     2581 tccgcgcccg acaacagcag cgcggcagcc gggctcttga gcggcgcgtg cagatcggaa
     2641 tcgaccaccc ctttcggcca gtccttgcag gcctcgacga gctgctgcac ctgctcggcg
     2701 cccatgtggg tggcgttgag cgcgaccaga tccagcttgg cctcatcgat gagcggcgcg
     2761 tcttcgctgc aggccacgct gttgtgcatg ccgtacgcaa acgccgcctg cagtgaatac
     2821 gcgaacacgc ggaactggct cgcgagcggc gtgaaattat cttcgtgtgt cgccaggtgc
     2881 agcgacagcg gcagcaatgc cgcctggtcg tcgttgtagc tcgcgaatct cagcaccgcg
     2941 gacaggtgcc gcgcggtgaa atcgaaatgg atcgggcggc ccgtggcagc gtcgctgacc
     3001 atcgtcttca tcggtttggc cgtgagctgt gaacgcagcg cccgatagtc tgccgatgga
     3061 tcagtgaaag ccttggcgca cgcggcatcg gcggagcatc gcccgagtat tcgttccagc
     3121 gcgcgttccg cgtcgatggc gatcgcgggt cccagcacca cttcgggatt cacgactcca
     3181 tcgagaatca ccgtgcgcgt ggatttcggg aagcggcgcg catagtgctg cgcgacgcgc
     3241 gtgccgtagg agttgccata cagattgatg agctggtacc ccagcgcctc gcgaaccttg
     3301 tcgaggtcgc gcaccgcgat gctggtggtg tacatgcgca ggtcggactt cttcgacaac
     3361 tcgtcacggc atttgatatt ggccgcgccc acttcgatct cgtcgatgcg ctcgaacaga
     3421 ttgtcgttgc cgtaatcgca gtcgagccgg tgcgagcgtc ccgtcccgcg ctgatccacg
     3481 aggatgatgt cgcgatcgcg gcgcacgcgc tcgaagggcc cggacgatga cgtatagaga
     3541 tccacggccg atgtgccggg accacccgcg atgagaaaca gcgggtcggt cttcttgttg
     3601 agactgatgg ccggcacgcg cgccacgaag agctcgatgg agcggccgcc cggcttgtcg
     3661 ggattttccg ggaccttgag cacgccacat tcggcgctca gcgccagcat gcgcgcggga
     3721 tgttcgagcc ggcacggttt gagcggaagc gccggcaccg cgttcgtcgc tgtcgcgatg
     3781 gcggcgaagc ccaggaatcc tgcgagagcg caaagtcgca tcatgcggcg cgcaggatac
     3841 acgagcccca aacgagcgcc gttgcgccac ccacgaaaaa gggcgtgacc ttgcgagtca
     3901 cgccctcatc gacttcgaga ttactgagcc gcgaacctta tccacgatcc tggcggtcgt
     3961 gcttctggcg atagatgcga cgcgactgct ggttcgcttc gtgctgcagg tgggcgcgct
     4021 cgcggcccgt gaccacacca tcggcttcgg cgcggcgctc gacgcggttc agatgcacct
     4081 ggccggtggc gaggcgacgg gtttcgcgca gggtcagctc accgctgccg acgccgttat
     4141 agatgcgctg gcgctggttc tgctggcgcg cgtcgtaacg gggggtgtcg gccgaagcag
     4201 tacccaccat cgcgagtgaa agggcgagac cgataccggc caaaagggtt gtcttgttca
     4261 tggttctctc ctgctgagcc gaaattcggc actgttgcca atacaacggg ctggcagcgg
     4321 tggcgttgac accccgggcg agctaccatt cgccgcctta actcattgat ctggcggcga
     4381 ttccgtgact tcggcaggcg tttttgacgt aatcatcgtt ggcggcgggc acgcgggcac
     4441 tgaagccgcg ctggcggctg cccgcatggg cgcgcgcacg ttactgctga cgcaatcgat
     4501 cgagacgctg ggccagatga gctgcaatcc ggccatcggc ggcatcggca agggacatct
     4561 cgtcaaagag atcgatgcgc tcggcggcgt catggcgcgc ggcgccgatg cggcggcgat
     4621 ccagtcgcgc acgttgaacg ccagcaaggg tcccgcggtg cgcgcgaccc gtgtacaggc
     4681 cgatcgcatt ctctacaggg ccttcatccg gcgcgcgctc gagaaccagc cgaacctcac
     4741 gctgttccag caggagtgtg gcgacctcat cgtcgacaac ggcgtggttc gtggagtcgt
     4801 gacggtcacc ggcatcgaat tccgtgcgcg ctcggtagtg ctcacggtcg gcacgttcct
     4861 cggcggccgc attcacgtgg gtctcgacaa ctattcgggt ggccgcgccg gcgatccgcc
     4921 gtctaacaaa cttgccgaac gcctgcgcgc attgccgttt cgtacggcgc gcctcaagac
     4981 cggcacgccg ccgcgcatcg acggccgcac catcgactac tcgcaacttg cgacacagtt
     5041 ctccgacgat ccgcgaccgg tgttcagcta cctgggtcgt acctccgatc acccgtcgca
     5101 gttgccctgc cacatcacgg cgacgaccga gcgaacgcac gagatcatcc gcgcggcgac
     5161 agatcgatcg cccatgttca caggcaagat cgaaggagtg ggtccgcggt actgcccgtc
     5221 cgtggaagac aaggttgtcc ggttcgcgga caagacctcg caccagatct tcatcgagcc
     5281 cgaaggactg aacacgcacg aggtgtatcc gaacggcatc tcgacgagct tgccgtacga
     5341 cgtgcagttc gaattcgtgc gcaccatcaa gggtttcgag aacgcgcaca tcacgcggcc
     5401 cggctacgcc atcgaatacg atttcttcga tccgcgcgat ctcaagtctt cgctcgaaac
     5461 gaaagtgctt gcgggcctct ttttcgcggg ccagatcaac ggcaccacgg gatacgagga
     5521 agccgcggcg cagggcctca tcgccggcat caacgcggcg cgagcagcgc gcgaacagga
     5581 tgcgttcatc ccgaagcgca gcgaaagcta cgtgggcgtg ctcatcgacg acctggtgac
     5641 gcgcggcacg cgcgagccgt atcgcatgtt caccagccgc gccgaacatc gcctgctgct
     5701 gcgcgaagac aatgccgatc tgcgactcac acccgccgga cgcgagctgg gcctcgtgga
     5761 cgatgcgcgc tgggcgttct tcgaatccaa gcgcgatggc agcgcgcgcg aactcgcgcg
     5821 cttcgagacc acgcgcatca aggccgccga cattcctgcg gcctggtcga cgcgagtcct
     5881 cggtgacacc accacgcgcg acgcgttgtc cgcgttcgat ctgctgcgcc ggcccgaagt
     5941 ttcgtatgac gacgtggtag agctcatcgg gcccatgaac gcggtcgatg acgaccgcct
     6001 tcctgcgcag atccgcgtgc agctcgaagt ggcggccaag tact
//
LOCUS       DATDEU010000297         5012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1949464, whole genome shotgun sequence.
ACCESSION   DATDEU010000297 DATDEU010000000
VERSION     DATDEU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949464"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VIV63_07145"
     CDS             <1..1168
                     /locus_tag="VIV63_07145"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY6124410.1"
                     /translation="WVLEYSPETFSMTELPVALDACNAATRAWDAGPGRPIIMNLPTT
                     VENATPNIFADQIEWMSTRLERREHVVLSVHTHNDRGTGVAAAELGMLAGAQRVEGCL
                     FGNGERSGNVDLVTLALNFYSQGIHPGLDFSDINSVARAVEEATRLPIHPRHPYVGDL
                     VFTAFSGSHQDAIKKGMAAQKPDALWEVPYIPVDPADLGRTYDSVIRVNSQSGKGGIA
                     YLLERDYGVVMPRRMQVEFSALVQREMDASETEMTSQQLWTLFESTYLATDFEVTYHA
                     HHLFEEGDKQGIELEVTVSGTRRKLRGVGSGPISATVAALALPIRIDNYEERALEAGT
                     DATALAIVEAARDGVTGIRYGAGRHANIATASVLAVLSAARRLGVSASDLELRR"
     gene            complement(1157..1591)
                     /locus_tag="VIV63_07150"
     CDS             complement(1157..1591)
                     /locus_tag="VIV63_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124411.1"
                     /translation="MLKHDLSTSDREFRAAFEAGAFAPADFSHRAHVRLAYVYLAESD
                     VDLALERMRAALVSFLSHHDIPASKYHATLTRAWILAVHHFMHRCPEAASADDFIERN
                     PLLLDSKIMLTHYSAELLFSDQARADFVEPNLDPIPRHRLTA"
     gene            complement(1647..2693)
                     /locus_tag="VIV63_07155"
     CDS             complement(1647..2693)
                     /locus_tag="VIV63_07155"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY6124412.1"
                     /translation="MKMSSTDQTERNIKNPVVAIAGATGAVGVELMQCLEQRKFPLVE
                     LRLLASARSAGKSMKFRGGDIAVQELTDASLEGVDIALFSAGGAQSKRFAPAAVRAGA
                     VVVDNSSFFRMNTDVPLVVPEINAAAMNKHNGIIANPNCVAIVAATPLWPLHQKRRIK
                     RITMSTYQAASGAGAAAMEELSESTRAHLEGRAFTPKVLPHPYAFNVFSHNTKVDPET
                     GYNEEETKVMQETRKIFGEPDLRVTATCVRVPVLRAHCIALNIECEEPLSVAEARAIL
                     AAAPGVRLVDDMERNYFPMPHDASGKDDVLVGRIRRDVSDPSGRTLALFVAGDQLRKG
                     AALNAVQIAERLLA"
     gene            complement(2933..3142)
                     /locus_tag="VIV63_07160"
     CDS             complement(2933..3142)
                     /locus_tag="VIV63_07160"
                     /inference="COORDINATES: protein motif:HMM:NF022587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEY6124413.1"
                     /translation="MIKILIIITLFAIIASLGSALFHLSRGKGDSKKMARALTIRVGL
                     SVALFILLMLAYYNGLITPHGIAPR"
     gene            3228..3905
                     /locus_tag="VIV63_07165"
     CDS             3228..3905
                     /locus_tag="VIV63_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY6124414.1"
                     /translation="MTLITLVLLTLFVGLGRWQWHRGEAKQAVWSEYERNAAASSIGT
                     REFDSIDRFARLELNGEFEPDHQFLLDNRSHAGKPGYEVLTPFRLADGRRVLVNRGWI
                     PFGGYRDRLPDVAMSTVAPALISGRIDDLPSGGLASGHAAPDANAPWPRLTSFPTHAE
                     LADSLGVKLENRMLLLDAGVPGGYLREWSPPGMPPSRHFSYAIQWWGFAAVLLVLYFG
                     LNFRKVS"
     gene            3905..4489
                     /locus_tag="VIV63_07170"
     CDS             3905..4489
                     /locus_tag="VIV63_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124415.1"
                     /translation="MQDSRARGRRTLLIVAAVFLLPVAVAFSLYYGKLWRPANSSSKG
                     DLIDPARPLTVSGLRHADGTPADISAFTGKWTLLYIGDGRCDDACRTALVFGRQTRLA
                     LNNEMTRVQRMILATGNCCDTDYFAREQAGLVALDASAPDAASFLAQFPEGREHTLFI
                     VDPLGNLMMRHDARVTTKDLLSDLKKLLKLSHIG"
     gene            4505..>5012
                     /locus_tag="VIV63_07175"
     CDS             4505..>5012
                     /locus_tag="VIV63_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY6124416.1"
                     /translation="MREIIWIRRLALIGAILCFGVVVFGGYTRLSNSGLGCPDWPGCF
                     GHIAPTGSAEHYASNADVRKAWIEMIHRYFASTLGLIIVVIAALSIRARREPGVSVTF
                     ALVLLVLVVLQGMLGMLTVTWLLKPLIVTGHLVGGLTTFALLLWLWLSKHAQGRPVDG
                     YTVLAGNRL"
BASE COUNT          860 a         1573 c         1630 g          949 t
ORIGIN      
        1 atgggtgctc gagtactcgc ccgagacctt cagcatgacc gaactgccgg tggcgctcga
       61 cgcctgcaac gccgcgacgc gcgcctggga cgccgggccc gggcggccca tcatcatgaa
      121 tctgccgacg acggtggaaa acgcgacgcc gaatatcttt gctgaccaga tcgagtggat
      181 gtcgacgcgc ctggagcggc gcgagcacgt ggtgctctcg gtgcacacgc acaacgatcg
      241 cggcacgggc gtagccgcgg cggagctcgg catgctcgcc ggcgcgcagc gcgtcgaagg
      301 ttgcctgttc ggcaacggcg agcgatcggg caacgtggac ctggtgacgc tcgcgctcaa
      361 cttctattcg cagggaatcc accccggcct cgatttctcc gacatcaact ccgtggcgcg
      421 cgccgttgaa gaggccacgc ggctgccgat tcatccgcgg catccctacg tcggcgatct
      481 cgtgttcacg gccttttccg gctcgcacca ggacgccatc aagaaaggca tggcggcgca
      541 gaaacccgat gcgctctggg aggtgccata tatcccggtc gaccccgccg acctcggacg
      601 cacgtacgac agcgtcatcc gcgtgaacag ccagtctggc aagggtggca tcgcctatct
      661 tctcgagcgc gactacggcg tcgtgatgcc acggcgcatg caggtggaat tcagcgcgct
      721 ggtccagcgc gagatggacg cgtcggaaac cgagatgacc tcacagcagc tctggacgtt
      781 gttcgagagt acgtatctcg ccaccgactt cgaagtgacc taccacgcgc atcacctgtt
      841 cgaagagggt gacaagcagg gtattgagct cgaagtcacc gtctcgggta cgcggcgcaa
      901 gctgcgcggc gtggggtcgg gccccatctc ggccacggtc gccgcgctcg cacttcccat
      961 ccgcatcgac aactatgaag agcgcgcgct cgaggcggga accgatgcaa ccgcgcttgc
     1021 gatcgtcgag gcggcgcgtg atggtgtaac gggcattcga tacggcgcgg gacgccacgc
     1081 gaacatcgcg acggcatccg tgctggcggt gttgtcggcc gcgcggcgtc ttggcgtcag
     1141 cgcgagcgac ctcgagttac gccgttaggc ggtgacgggg gatcggatcg aggttcggct
     1201 cgacgaagtc cgcacgcgcc tggtccgaga acaacagttc cgccgagtaa tgcgtcagca
     1261 tgatcttgct gtcgagcagc agcggattgc gttcgatgaa atcgtccgcg gacgccgctt
     1321 ccggacagcg atgcatgaag tgatggacgg cgaggatcca cgcgcgcgtg agcgttgcgt
     1381 gatacttcga tgccggaata tcgtggtgcg agagaaagct cacgagcgcg gcgcgcatgc
     1441 gttccagtgc gaggtcgacg tcgctctcgg ctagatagac gtacgcgagt cgaacgtgcg
     1501 cgcgatggga aaaatccgcg ggcgcaaatg cgcccgcttc aaaagccgca cgaaattcac
     1561 gatcactggt cgataagtcg tgtttcaaca tgtcggcatg gcctcgctaa ctcttcctct
     1621 catcccccca tcgaaatctt ccgcgctcag gccagcagcc gctccgcgat ctgcaccgcg
     1681 ttgagcgccg cacccttgcg cagctggtcg ccggccacga acagcgcgag cgtgcgaccc
     1741 gatggatcgc tgacgtcgcg gcggatgcgg ccgaccagca catcgtcctt gccgctcgcg
     1801 tcgtgcggca tcggaaaata gttgcgctcc atgtcgtcga ccaaccggac tcccggagcg
     1861 gcggcaagga tagcgcgtgc ttcggcaacc gacagcggtt cttcgcactc gatgttcagc
     1921 gcgatgcaat gtgcacgcaa aacgggcacg cgcacgcagg tggcggtgac gcgcaggtcg
     1981 ggttcgccga aaatcttgcg cgtttcctgc atcaccttgg tctcttcctc gttgtatccg
     2041 gtttccggat cgaccttggt gttgtgcgag aacacgttga acgcatacgg gtgcggcagc
     2101 accttcggcg tgaacgcgcg gccttcgaga tgcgcccgcg tggactcgct gagctcttcc
     2161 atggcggcgg cgccggcgcc ggaagcggcc tggtacgtgg acatggtgat ccgcttgatg
     2221 cggcgcttct ggtgcagcgg ccacagcggc gtggcggcaa cgatggccac gcagttcgga
     2281 ttcgcgatga tgccgttgtg tttgttcatg gccgcggcgt tgatctcggg aaccacgagc
     2341 ggcacgtcgg tgttcatgcg gaagaacgac gagttgtcga cgaccaccgc accggcgcgc
     2401 acggcggcgg gagcgaaacg cttcgattgc gcgccaccgg cggagaacag cgcgatgtcc
     2461 acgccttcga ggctggcgtc cgtgagctcc tgcacggcga tgtcgccgcc gcggaacttc
     2521 atcgattttc cggcggaccg ggccgaggcg aggaggcgca gttcgacgag cgggaatttg
     2581 cgctgctcga ggcactgcat gagctcgacg ccgacggcgc cggtggcgcc cgcaatggcg
     2641 acgacgggat tcttgatgtt gcgttcggtc tgatcggtcg atgacatttt catgggctcc
     2701 aaatgaaaaa ggccccggga gtttcctcgc ggggccttcg ggtagttctt tgccgttcag
     2761 atcaacgcgc gcaaatcccc aaggcccctg cgggcttggt cttgagcgtc tttttgatcg
     2821 tgttgcgatt catgcggggg cggaatgtag cggagtagtt cgctgccgta aagcaaaaac
     2881 cggcaggtcc gacccctttt tctaggaaat ggggagtgtc cccatttcct tttcagcggg
     2941 gagcgatgcc gtggggggtg atgaggccgt tgtagtaggc cagcattaac agaatgaaga
     3001 gcgcgaccga caggcctacg cggatggtca gggcccgggc catcttcttc gagtcgccct
     3061 tgccccggga aaggtgaaac aacgcgctgc caaggcttgc gatgatggcg aacagcgtga
     3121 tgatgatgag gattttgatc acgtccataa gggcgcactt tatactcccg gcccccatgc
     3181 cgacccgact ttccgcgttc ggaagggtct tcgcgccttc ctggcccatg accctcatca
     3241 ccctcgtcct gctgacactt ttcgtcgggc tgggccgatg gcagtggcac cgtggcgaag
     3301 cgaagcaggc ggtgtggagc gagtacgaac gcaacgcggc ggcatcaagc atcggcactc
     3361 gcgagttcga cagcatcgat cggttcgccc gcctggagtt gaacggagag ttcgaacccg
     3421 atcaccagtt cctgctggac aaccggtcgc acgcgggcaa gcccgggtac gaagtgctca
     3481 ctccatttcg gcttgccgat ggacggcgag tgctcgtcaa tcgcggctgg attccatttg
     3541 gcgggtatcg cgaccgcctc cccgatgtgg ccatgtcgac tgtcgcgcct gcgctgatct
     3601 cgggccgcat cgacgatttg ccgagcggcg ggctggcgag cggtcatgcc gcgccggatg
     3661 ccaacgcgcc ctggccgagg ctcacgagtt ttcccacaca tgcggaactg gcggactcgc
     3721 tgggcgtcaa gctcgagaac cggatgttgc tgctcgacgc cggcgtgccc ggcggttacc
     3781 tgcgagagtg gtcaccgccc ggcatgccgc catcgcgaca cttctcttat gcaatccaat
     3841 ggtggggttt tgcagccgtg ctgctcgtgc tctacttcgg cctcaatttt cgcaaggtgt
     3901 cctgatgcaa gattcccggg cgcgcggtcg gcgcactctg ctcatcgtcg cagccgtttt
     3961 cctgctgccc gtggcggtag cgttctctct ttattacggc aagctctggc gtcccgcgaa
     4021 ctcgtcgagc aaaggcgatc tcatagatcc ggcacggcca ctgaccgtga gcggtctgcg
     4081 ccacgcggac ggcactcccg ccgacatttc ggcgttcacg ggcaaatgga cactccttta
     4141 tataggtgac ggccgctgtg acgacgcctg ccgcacggcg ctggttttcg gccggcaaac
     4201 gcggctggcg ctcaacaacg agatgacccg cgtgcagcgc atgatcctcg ccaccggcaa
     4261 ctgctgcgat acggactatt tcgcgcgcga acaggccggc ctcgtcgcgc tcgatgcgtc
     4321 ggcaccggac gctgcgtctt ttctcgcgca gtttcccgag gggcgcgaac acacgctgtt
     4381 catcgtcgat ccgctcggca acctcatgat gcgccacgac gcgcgcgtca cgaccaaaga
     4441 cctgctcagc gatctcaaga agctgctcaa gctctcgcac atcggctgag actctcccgg
     4501 acacatgcgc gaaataatct ggatccgacg gctcgcgctc atcggcgcga tcctgtgttt
     4561 cggcgtggtc gtgttcggtg gctacacgcg gctgtcgaat tccggtctcg gctgtcccga
     4621 ctggcccggc tgcttcggac acatcgcgcc cacgggcagc gcggagcatt acgcgagcaa
     4681 tgccgacgtg cgcaaggcgt ggatcgagat gatccatcgc tacttcgcga gcacgctggg
     4741 tctcatcatc gtcgtgattg ccgcgttgtc gattcgcgcg cggcgtgaac ccggcgtgag
     4801 cgtcaccttc gcgctggtgc tgctggtgct cgtggtgttg cagggcatgc tcggcatgct
     4861 caccgtcacc tggctgctca agccgctgat agtcaccggg cacctggtgg gcgggctaac
     4921 taccttcgcg ctgctgttgt ggctctggct gtcgaagcat gcccagggac ggccggtgga
     4981 tggctacacc gtactcgccg gcaaccggct gg
//
LOCUS       DATDEU010000298         2918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1366513, whole genome shotgun sequence.
ACCESSION   DATDEU010000298 DATDEU010000000
VERSION     DATDEU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366513"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VIV63_07180"
     CDS             complement(<1..218)
                     /locus_tag="VIV63_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124417.1"
                     /translation="MSERLTKLTTILAWTAAAAFAASWFLPVIPDVPGWMAFRYALAP
                     LVPFRDAGAIAWDDSAPQVMSALTNVVF"
     gene            complement(234..722)
                     /locus_tag="VIV63_07185"
     CDS             complement(234..722)
                     /locus_tag="VIV63_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124418.1"
                     /translation="MNKSIFVAISVFALAACETPNGAGTGSGNVRGTQKPVAFTWESD
                     DSNSGDITATFGSGRVFTGEYVQISRDISEKNLDPLWDGWDRPRSKNEWRYWNASSRS
                     EFVRENSGRVLANLHDAKGEYMRCRFTLANPERGMKGGGEGRCQLSDSGKEINAEFER
                     QS"
     gene            complement(772..1395)
                     /locus_tag="VIV63_07190"
     CDS             complement(772..1395)
                     /locus_tag="VIV63_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY6124419.1"
                     /translation="METPTAYHEGMRELQDLRETRALADRLEKVTMRAAFTEDDQAFI
                     QRCRMFFLATADTQGQPDCSYKGGLPGFVRVLDDRTLAIPDYDGNGQYRSWGNVLVNQ
                     QVGLLFVDFETPKRLRVNGAAVVSHDDPLLAEFPGAVLLVRVTAQRIFPNCPRYIHKM
                     QLVEESVYSPRPQYTPPVPAWKTFDVFKDALPARDRPTETRDKTDKP"
     gene            complement(1404..2099)
                     /locus_tag="VIV63_07195"
     CDS             complement(1404..2099)
                     /locus_tag="VIV63_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY6124420.1"
                     /translation="MLDIREDDIEMILRLLAALGSGALIGYERSFHGRPAGLRTHVLV
                     CLASALLMLVTVYEDHWVRSQGESRLDPTRMAQGIMTGIGFLGAGVIVKEGLNVRGLT
                     TAASIWITAAIGVIAGVGLYLPLVVSVILTLAVLSVFRWIEMRVPTQAYFNFDVKYEN
                     DGNLSEAEMRALIQKHKFSIANFSYRLDSEGRVLRHSMTLQTTDRSAASRLAHTLKEN
                     PTVLEFRLSPTGD"
     gene            complement(2110..2493)
                     /locus_tag="VIV63_07200"
     CDS             complement(2110..2493)
                     /locus_tag="VIV63_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124421.1"
                     /translation="MKNTSAFAMAIAFALLAGCQSATVPGPSPRGPTTKNFKYGLGDG
                     QTMATAVEIRTRSETDGGIMIMDWIKQRYPGYAITEQELIEQRDRAYNMITIIGPSNT
                     PQSIYFDISTYYRRIGNDNFPKPLG"
     gene            complement(2530..>2918)
                     /locus_tag="VIV63_07205"
     CDS             complement(2530..>2918)
                     /locus_tag="VIV63_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124422.1"
                     /translation="FDTFSYKSYLLLPRNFREFWPRHDVPTPEPRAALINQLATETYG
                     QDWNPERGVVVRSGQKRMRATAAPLVLGPGADPALEFFSRANPGHADGDMLVCLCPLT
                     LSNWISVGRKALERLHRPGMIDGGRI"
BASE COUNT          504 a          901 c          964 g          549 t
ORIGIN      
        1 atgaacacga cgttggtgag cgcgctcatg acctgcggcg cggaatcgtc ccaggcaatc
       61 gcaccggcgt ctcggaaagg caccagcggc gccagcgcgt atcgaaacgc catccatccc
      121 gggacgtcag gaatgaccgg caggaaccac gaggcggcaa acgccgccgc cgcggtccag
      181 gccagtatcg tcgtcagttt cgtcaggcgc tcagacactc ctcacggagc gtatcaagac
      241 tggcgctcga actccgcgtt gatttccttg cccgagtccg aaagctggca gcggccttcg
      301 ccaccaccct tcatgccgcg ttccggattg gcgagcgtga aacggcagcg catgtattcg
      361 cccttcgcgt cgtgcaaatt ggcgagcacg cggccgctgt tttcccgcac gaactcggaa
      421 cgcgaactgg cattccagta acgccactcg ttcttgctcc gcgggcggtc ccagccgtcc
      481 cacagcgggt ccaggttctt ctcgctgatg tcgcgcgaga tctgaacgta ttcgccggtg
      541 aacacacggc cgctgccgaa cgtcgcggtg atgtcaccgg aattgctgtc gtcgctttcc
      601 caggtgaatg ccacgggttt ctgcgtgccg cgcacgttgc cggaaccggt tcccgcgccg
      661 ttgggagttt cgcaagcggc cagcgcgaag accgagattg cgacgaagat tgatttgttc
      721 atggtggcat tgtgggtgcg ccgccgcggg cgcgactgcc agacaaacaa gtcagggctt
      781 gtcggttttg tcgcgggttt ccgtaggacg atcgcgcgcc ggcagcgcgt ccttgaatac
      841 gtcgaaagtt ttccaggccg gcaccggcgg cgtgtactgc ggccgcggcg aatacaccga
      901 ctcttcgacg agctgcatct tgtgaatgta gcgcgggcaa ttggggaaga tgcgctgggc
      961 cgtcacgcgg accagcaaca ccgcgcccgg gaactccgcc agcagcggat cgtcgtgact
     1021 aactaccgcg gcgccattca cgcgcaggcg cttgggtgtt tcgaagtcca cgaacagcag
     1081 gcccacttgc tggttgacca gtacgttgcc ccagctccgg tactggccgt tcccgtcgta
     1141 gtcgggaatt gcgagagtac ggtcgtcgag cacgcgcacg aaccccggca atccgccctt
     1201 gtacgagcag tcgggctggc cctgggtatc ggcggtggcg aggaagaaca tgcggcagcg
     1261 ctggatgaac gcctggtcgt cttcggtgaa cgcggcccgc atcgtgactt tttccagccg
     1321 gtcggcgagc gctcgcgttt cgcgaaggtc ctgcagctcg cgcattccct cgtggtaagc
     1381 cgttggggtt tccatgcgac ctctcagtca ccggtgggtg acaggcggaa ctcgagaacc
     1441 gtcggatttt ccttgagcgt gtgcgcgagc cgcgaggcgg cgctgcggtc ggtggtctgc
     1501 agcgtcatgc tgtggcgaag cacgcgaccc tcggagtcga gccggtagct gaaattggcg
     1561 atgctgaact tgtgcttctg gatgagcgcg cgcatctccg cttccgagag gttgccgtcg
     1621 ttctcgtatt tcacgtcgaa gttgaaatac gcctgtgtcg gcacgcgcat ctcgatccag
     1681 cggaacaccg acagcacggc gagcgtcagg atgaccgaca ccaccagcgg caggtacagg
     1741 ccgacgccgg caatcacgcc gatggcggcg gtgatccaga tggacgcggc cgtggtgagg
     1801 ccgcgcacat tgaggccttc cttgacgatg acgccggcgc cgagaaagcc gatgccggtc
     1861 atgatgccct gcgccatgcg cgtgggatcg aggcgggact caccctggct gcgcacccaa
     1921 tggtcctcgt acacggtcac cagcatcagc agcgcggatg cgaggcatac gagcacgtgg
     1981 gtacgaaggc ccgccggccg gccatgaaac gaacgttcgt agccgatgag tgcaccggac
     2041 ccgagcgcgg ccagcagccg caggatcatc tcgatgtcgt cttcgcggat gtccagcatg
     2101 cgcgtgcggt cagcccaacg gcttagggaa attgtcgttg ccgatgcgac ggtagtaagt
     2161 cgagatgtcg aagtagatgc tctgcggcgt gttgctgggc ccgatgatgg tgatcatgtt
     2221 gtaggccctg tcgcgctgtt cgatgagctc ctgctccgtg atcgcgtagc ccgggtatcg
     2281 ctgcttgatc cagtccatga tcatgatgcc gccgtccgtc tcgctgcgcg tccggatttc
     2341 cacggcggtc gccatggtct gcccgtcgcc gagtccatat ttgaaattct tggtggtcgg
     2401 tcctcttggc gaggggccgg gaacggtcgc cgactggcag ccggctaaca atgcaaatgc
     2461 gattgccatc gcgaaggcgc tggtgttttt catgggtact ctctcggtcc ttgtcggcaa
     2521 agggtcgcgc tagatgcgac cgccgtcaat cattccgggc cggtgcagcc gctccagcgc
     2581 cttgcgcccc acgctgatcc agttcgacag ggtcagcggg cagaggcaaa ccagcatatc
     2641 gccatccgcg tggcccggat tggcccggga aaagaactcg agcgccggat ccgcgcccgg
     2701 accgaggacc agcggcgcgg cggtggcgcg catgcgcttc tgaccggacc tgaccacgac
     2761 cccgcgttcg ggattccagt cctgtccata ggtctcggtg gccagctgat tgatgagcgc
     2821 ggcgcgcggc tcgggagtgg ggacgtcgtg gcgcggccag aactcgcgga agttgcgcgg
     2881 cagcagcagg taactcttgt agctgaaggt gtcgaaga
//
LOCUS       DATDEU010000299         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_491909, whole genome shotgun sequence.
ACCESSION   DATDEU010000299 DATDEU010000000
VERSION     DATDEU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491909"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2051)
                     /locus_tag="VIV63_07210"
     CDS             complement(<1..2051)
                     /locus_tag="VIV63_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6124423.1"
                     /translation="MSDFSMRRLHPLLQAQLRELRARVTGGRVSAHELLEMLSRYYDT
                     VDDERRAMVRSMQLMSDDARSLGVEIAEQGAAQLQVILDHIKDVVITVNAEGVIDRAN
                     PMTERVFDYPPGELSGQNIAVLVPAIAHDGSVQAGLSRLTESSDTFRGMRVSPEVTAR
                     RSDGTPFPAEIAVSRAGHGRGEVFVICLRDISERASAQEALRDSEARYRSLVDHAPEA
                     ITVIDPDSQSFVEANEPALRLFKMTRDQLLAVHLDAVSAEMQPDGQPANAPRQHLKLA
                     AAGESQVFEWIHRDSTGRDIPCEVRLARLQGGSKALVRASITDISERKRAEIIMENER
                     AFFALLASNAGLPAVLDVISALVQAVYPRCRCTISVLSPDGSCFALTIARHLPPMLAA
                     VLERTPIEPRRGSCAAAVYSACDVFVPDVANDAHWADRRQVVLDSGYRAVWSMPIKGA
                     SGKLLGSVAIYRPEPGLPDQREQVLQSHATRLAALAIERNLAEEALRASEAKFRGLFE
                     GVIEGVYQSTRDGRLVSVNSAFVKMLGYSSAEEMYALPGSVMLYWSATDRADFVRRVD
                     SDGEVRSMEVVLRRRDGTQVVALENSRGVRDGSGRIVGYEGTVSDITERKRAEQAIFA
                     EKDRALVTLQSIGDAVISTDANARIDYLNPVAERLTGWSVHEARGCPIGDVLQLIDES
                     TR"
     gene            complement(2204..>2489)
                     /locus_tag="VIV63_07215"
     CDS             complement(2204..>2489)
                     /locus_tag="VIV63_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124424.1"
                     /translation="TALPVEVALVPHQKFTMLRVHFETHEGLTIATGDTYGPVTDVSS
                     EKPLTHPLVLLPSKEGMFMVTVAVETEGDEGNITRIFSIPVIVSASAAAR"
BASE COUNT          427 a          808 c          829 g          425 t
ORIGIN      
        1 cgcgtggatt cgtcgatgag ctgcagcacg tcgccgatcg gacagccgcg tgcctcgtgc
       61 accgaccagc ccgtgaggcg ttcggcgacc ggattcagat agtcgatgcg ggcgttggca
      121 tcggtgctga tcacggcgtc accgatcgac tgcagcgtga ccagcgcgcg atccttttcc
      181 gcgaagatcg cctgctcggc gcgcttgcgt tcggtgatgt cggagaccgt gccttcgtag
      241 ccgacgattc gtccgctgcc gtcacgcacg ccgcgcgaat tctcgagcgc cacgacctgc
      301 gtgccatcgc gccggcgcag cacgacttcc atggagcgca cttcgccgtc cgagtcgacg
      361 cgccggacga aatcggcgcg gtcggtcgcg ctccagtaga gcatcaccga acccggcaat
      421 gcatacatct cttcggccga gctgtagccc agcatcttca cgaatgcgct gttcaccgag
      481 accagccggc catcgcgcgt gctctggtac acaccttcga tgacgccctc gaacagtccg
      541 cggaatttcg cctcgctggc gcgcagcgct tcctcggcga gattgcgctc gatggcgagc
      601 gccgccaggc gggtcgcatg cgactggagc acctgctcgc gctgatcggg cagacccggc
      661 tcggggcggt agatggccac cgagcctaac aacttgccgc tcgcgccctt gatgggcatc
      721 gaccagacgg cgcgatatcc ggaatcgagc accacctggc gacggtcggc ccagtgcgcg
      781 tcgttggcca cgtcgggaac gaacacgtcg caggccgaat agacggcggc ggcgcacgaa
      841 ccgcggcgcg gttcgatggg agtgcgctcg agcactgccg ccagcatcgg cggcagatga
      901 cgggcgatcg tcagcgcgaa gcaacttccg tcgggcgaca acacggagat ggtgcagcgg
      961 caacgcgggt acacggcctg cacgagcgcg gagatgacgt cgagcaccgc tggcagtccg
     1021 gcattggagg cgagcagcgc gaagaaggcg cgctcgttct ccatgatgat ctccgcgcgc
     1081 ttgcgctcgg agatgtcggt gatgctggcg cgcaccagcg ccttcgagcc gccctgcaac
     1141 cgggcgagcc ggacttcgca gggaatgtcg cggccggtgg aatcgcgatg gatccactcg
     1201 aacacctgcg actcgccggc cgcggcgagt ttcagatgtt gccgtggcgc gttcgcgggc
     1261 tgaccgtcgg gctgcatttc ggcgctgacc gcgtcgaggt ggacggcgag caattggtcg
     1321 cgcgtcatct tgaacagccg cagcgcaggt tcgttggctt ccacgaaact ctgcgaatcg
     1381 ggatcgatga cggtgatggc ttccggcgcg tggtccacca gcgagcgata tcgcgcctcg
     1441 ctgtcgcgca acgcttcctg tgcgctggcg cgttcggaga tgtcacgcag gcagatgacg
     1501 aacacttcgc cgcggccatg gcccgcgcgg ctcaccgcta tttccgccgg gaacggcgtg
     1561 ccgtcactgc ggcgcgcggt gacttcgggg ctcacgcgca tgccgcggaa cgtgtcgctc
     1621 gattcggtga gccgggaaag ccccgcctgg accgacccat cgtgcgcgat cgccggcacg
     1681 agtacggcga tgttctgccc tgacaattca ccgggtggat agtcgaacac gcgttcggtc
     1741 atcggattgg cgcggtcgat gacgccttcg gcgttgacgg tgatcaccac gtccttgatg
     1801 tggtcgagga tgacctgcag ctgggccgcg ccctgctcgg caatctcgac gccgagcgaa
     1861 cgggcgtcat ccgacatgag ctgcatggac cgcaccatcg cgcgacgctc gtcgtccacc
     1921 gtgtcgtagt agcggctcag catctccagc agttcgtggg cgctgacgcg tccgcccgtc
     1981 acccgcgcac gcaactcccg caactgcgcc tgcaacagcg gatgcaggcg ccgcatggag
     2041 aaatcgctca tatccacgcg gccctgagcc gatgccgtgg aatcagagct tccgtcagtg
     2101 ggggtgccct cggcgggttt tgacataaca atgcaagcga agaccaaatt gcatgatatg
     2161 tccgcgaacg ggcgtaccgc gtgaagcggc tcccggtttt gactcagcgc gcggctgcgg
     2221 aggccgagac gattacggga atggagaaga tccgcgtgat gttgccctca tcgccttcgg
     2281 tctcgacggc cacggtcacc atgaacatgc cctccttgga gggaagcagc acgagcgggt
     2341 gagtgagcgg cttttcgctg ctgacatcgg tcaccggccc atacgtatcg ccggtggcga
     2401 tggtcaaccc ctcatgggtc tcgaaatgca cgcgcaacat ggtgaacttc tgatgcggaa
     2461 cgagagcgac ctcgactggc aaggcggta
//
LOCUS       DATDEU010000300         4855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1220503, whole genome shotgun sequence.
ACCESSION   DATDEU010000300 DATDEU010000000
VERSION     DATDEU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220503"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VIV63_07220"
     CDS             complement(<1..496)
                     /locus_tag="VIV63_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEY6124425.1"
                     /translation="MRALEDELPGEHFNMWVRPLQAVESSGALKLLAPNRFAVDWVNA
                     HLLSRLGEIVRRFVEGDAPIVTVEVGSRAPELVQPVEPAANGAPRPKRSEGIVVGVRL
                     NPDFTFNGFVEGKSNQLAKAAAVQVAENPGRAYNPLFIYGGVGLGKTHLMQAVGHTIK
                     ARDPE"
     gene            701..835
                     /gene="rpmH"
                     /locus_tag="VIV63_07225"
     CDS             701..835
                     /gene="rpmH"
                     /locus_tag="VIV63_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY6124426.1"
                     /translation="MKRTYQPSKIKRKRATGFRARMKTTGGKKVLARRRSKGRKKIVP
                     "
     gene            867..1238
                     /gene="rnpA"
                     /locus_tag="VIV63_07230"
     CDS             867..1238
                     /gene="rnpA"
                     /locus_tag="VIV63_07230"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY6124427.1"
                     /translation="MQPTPRRPGFGAQLRLRSKLQFDAIYAGGRRIDDRFFGLRVKPN
                     GLTHPRVGLAVAVKTAGNAVKRNRLRRVVKESFRLAQFDLPAVDIVVAAKFQAREASP
                     ASLRASLATLWQRVATTCAPS"
     gene            1223..1459
                     /gene="yidD"
                     /locus_tag="VIV63_07235"
     CDS             1223..1459
                     /gene="yidD"
                     /locus_tag="VIV63_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY6124428.1"
                     /translation="MRSVLKLLIRGYQLAISPLLGPRCRFYPSCSHYAIEAIETHGAT
                     RGVWLTAKRISRCHPWHEGGFDPVPACLNHSHDQ"
     gene            1449..3161
                     /gene="yidC"
                     /locus_tag="VIV63_07240"
     CDS             1449..3161
                     /gene="yidC"
                     /locus_tag="VIV63_07240"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY6124429.1"
                     /translation="MTNNIRVFLWLSLLLALWLNYSQWQIDYGAKAATSASATDITGA
                     PKPADINDSVPQVAQTAATGTTPTDAVPTASTPETAAVPSAVESTGARKIRVTTDVLA
                     LDISLTGGTLVRAELPGYPIVKGEAAPVVLFNTDQASTNYVLRTGLSNPKGADAPTHQ
                     ATFAAAADSYTLAPGQTELRVPLTWTDGQGITVTKTFVFRRGQFIIDLEYEIDNASQA
                     PWSAASYARIQRLDPPVERSMFKVESYATRGPVIYDGTKYRKLDIESKEDTTLAIDVT
                     NGWIGGMQHHFVSAVVPNHETPQKVTLAVTGREYFLTTVSATAETVAPGTKKTLKEGL
                     FVGPKLQAQLETMHPELSRVADYGALTLLSKPLFWLLDHAHSIVKNWGLAIILVTFLL
                     KLLFYPLSEKAGRSMARMRALAPRMKALQETYKDDRTKLGQATMELYKTEKVNPLAGC
                     LPMLIQMPVFLAFYWVLLESVEMRQAPFFGWINDLSARDPFFILPVLNGLAMWGQSKL
                     NPPPPDPVQARIFQLMPIVFSVMFALFPAGLVLYWVTNTGLSILQQWNINRKITAEDK
                     ARKK"
     gene            3248..4600
                     /gene="mnmE"
                     /locus_tag="VIV63_07245"
     CDS             3248..4600
                     /gene="mnmE"
                     /locus_tag="VIV63_07245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY6124430.1"
                     /translation="MSHPDTIVAAASPPGRGGVGVVRVSGPKTPEIAATLVGELPKPR
                     HATLSRFVDRAGNPIDIGIALFFPGPLSYTGEHVLELQGHGGPIIVEQLIERCLELGA
                     RRARPGEFTERAFHNDKIDLAQAEAVADLIDAGSREAARAAMRSLQGEFSGMVQGLTD
                     SLIDLRTYVEAAIDFPEEEVDFLGDKELHERLATVRAHFDAVSAAASQGRLLRDGMTV
                     VLAGRPNSGKSSLLNRLAGYEAAIVTAIPGTTRDVLRERIDIDGLPLHVLDTAGLRDS
                     DDVVEREGVRRAAAEMSRADRVLFVIDTSTDPHGRAFELERERLPADVPVTLIFNKID
                     LGEGLPVGDTISGPPRIHVSAVTGKGLDLLRAHLKDSVSFQTAGSGTVSARRRHLEAL
                     ARARECVETAASTLNQRRGGELVAEELRQGQQALEEITGIFSADDLLGRIFGSFCIGK
                     "
     gene            4602..>4855
                     /locus_tag="VIV63_07250"
     CDS             4602..>4855
                     /locus_tag="VIV63_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124431.1"
                     /translation="MWVGNRCYPPTDVQQALESLFGESVGQIKVIQYSIFVRFHWRAI
                     ATTRPNRIYLRGSGKEFFADGPLVLHEYFHVIRQWGTGDMT"
BASE COUNT          893 a         1602 c         1487 g          873 t
ORIGIN      
        1 cttccggatc gcgcgccttg atggtgtgac ccacggcctg catgagatgc gtcttgccga
       61 gaccgacgcc cccatagatg aaaagcgggt tgtaggcgcg gccaggattt tctgcgacct
      121 gcaccgccgc ggccttggcg agctgattgc tcttgccctc gacgaaaccg ttgaacgtga
      181 aatccggatt gagccgaact ccgacgacga tgccttcgct gcgttttggg cgcggagcgc
      241 cgttggcggc gggctcaacg ggctgcacca gctccggcgc tcgcgacccc acctcgacgg
      301 tcacgatcgg ggcgtctccc tcgacgaacc gccgcacgat ctcgcccaga cgcgacaata
      361 agtgggcatt cacccagtcc acggcaaagc ggttgggtgc gagcagcttg agggctccgc
      421 tgctctcgac cgcctgcagg gggcgcaccc acatgttgaa atgctcaccc gggagctcgt
      481 cttccagagc tcgcacgcaa cgcgtccaga gcgtgccgcc atccagctgt gatgtatcgc
      541 gagacgctgc agtcacaggt ggatccgttt gggactcggg ccggaaaaaa gggtgcgaag
      601 tctagccttt cctggccgga ttgtattgac acccgaaggc ccggctgcgt aaccttcccg
      661 cccttttttc cgggatatca gcggcttcgg cccgatcacc atgaaacgca cttatcagcc
      721 gagcaagatc aagcgcaaac gcgccacggg ttttcgggcc cgcatgaaaa ccacgggtgg
      781 gaagaaggtc cttgcacgcc gccgttccaa gggccgcaag aagatcgttc cctaaagacg
      841 cgagagctcg tcttcagcgg gccgcaatgc agcccacacc acgtcgccca ggctttggcg
      901 cgcagctgcg cctgcgcagc aagttgcagt tcgatgcgat ctacgccggt ggccggcgta
      961 tcgacgaccg attcttcgga cttcgcgtca agcccaatgg gctcacgcac cctcgcgttg
     1021 gtctcgccgt cgccgtcaaa accgcgggca atgccgtgaa gcgaaatcgc ctgcggcgcg
     1081 tggtcaagga gagcttccgt ctcgctcaat tcgacctgcc cgctgtcgac atcgtggtcg
     1141 ccgcaaaatt tcaagcgcgt gaagcatcgc cggccagctt gcgtgctagc ctcgcgaccc
     1201 tttggcaacg cgtcgcaacc acatgcgctc cgtcctgaaa cttctgattc gcgggtatca
     1261 gctcgccatc agcccgttac tcgggccgcg ctgccggttc tatccgtcgt gttcgcacta
     1321 cgcgatcgaa gccatcgaga cgcatggcgc cacgcgcggt gtgtggctca ccgccaagag
     1381 aatttcccgt tgtcacccct ggcacgaggg cggattcgat ccggtcccgg cctgcctcaa
     1441 tcattcgcat gaccaataac atccgtgtct tcctgtggct gagtctcctg ctcgccctgt
     1501 ggctcaacta ttcgcagtgg cagatcgact acggcgcgaa ggcggccacg agcgccagcg
     1561 ccactgatat caccggcgct ccgaaaccag ccgatatcaa cgactctgtt ccgcaggtgg
     1621 ctcaaactgc ggcgacaggg actacgccca cggatgcggt tccaaccgcc tccacgcccg
     1681 agaccgccgc cgtgccgtcc gctgtcgaga gcaccggcgc ccgaaagatc cgggtgacca
     1741 ccgacgtgct ggccctggat atctccctca ccggcgggac actggttcgc gccgaattgc
     1801 cggggtaccc gatcgtcaaa ggtgaagccg ccccggtggt gttgttcaac accgaccagg
     1861 cttccactaa ctacgtcctg cgcaccgggc tatccaaccc gaagggagcc gatgcgccca
     1921 cgcaccaggc gacctttgca gccgcggccg acagttacac gctggcgccg ggtcagacag
     1981 agttgcgcgt cccactgacg tggaccgacg gccaaggcat cacggtcacc aagacgttcg
     2041 tcttcaggcg cggtcaattc atcatcgacc tcgagtacga gatcgacaac gccagccagg
     2101 ccccgtggtc ggcagcttct tacgcgcgca ttcagcgcct ggatccgccc gtcgaacgct
     2161 cgatgttcaa ggtcgagagt tacgcgactc gcggaccggt gatctacgac ggcaccaaat
     2221 accgaaagct cgacatcgaa agcaaggaag acacgacgct ggcgatcgac gtgacgaacg
     2281 gctggatcgg cggcatgcag catcacttcg tgagcgccgt cgttccgaat cacgagacac
     2341 cgcagaaggt gaccctcgcg gtgaccggac gcgagtattt cctgacgacc gtgagcgcga
     2401 ccgcggagac cgtggcgccg ggcacgaaga agacgctgaa ggaagggctg ttcgtcgggc
     2461 cgaagctgca ggcgcagctc gagacgatgc atcccgaact cagccgcgtc gccgactatg
     2521 gcgcgctcac gttgctttcg aaaccgctgt tctggttgct cgaccacgcg cattcgatcg
     2581 tgaagaactg gggcctggcc atcatcctgg tcacgttcct gctgaagctg ctcttctatc
     2641 cgctgtccga gaaagccggt cgctcgatgg cgcgcatgcg cgcgctcgcg ccacgcatga
     2701 aggcgctgca ggaaacctac aaggacgacc gcaccaagct cggccaggca accatggagt
     2761 tgtacaagac cgagaaggtg aacccgctcg ccgggtgctt gccgatgctc atccagatgc
     2821 cggtgttcct ggcgttctac tgggtgctgc tcgagagcgt ggaaatgcgc caggcgccgt
     2881 tcttcggctg gatcaacgac ctgtcggcgc gtgatccgtt tttcatcctg ccggtcctga
     2941 acggcctggc gatgtggggt cagtcgaaac tgaatccgcc gccgccggat ccggtccagg
     3001 cgcgaatctt ccagctcatg cccatcgtgt tctcggtgat gttcgcgttg ttccccgccg
     3061 gcctcgtgtt gtactgggtg acgaacaccg gcctgtccat cctgcagcag tggaacatca
     3121 accggaaaat cacggccgaa gacaaggcgc ggaagaaata gccgcgcctg tctcccgagg
     3181 ctgatgcgct gcagcgtgcg gcaacgggcg tagactgccc gtcggcggtg cctgtgccgc
     3241 tgcattcatg agtcaccccg acaccatagt ggcagcggca tctccgcccg gacgcggcgg
     3301 cgtcggtgtc gtgcgtgtgt cggggcccaa gactcccgag atcgccgcga cgctcgtcgg
     3361 cgaattgccc aagccccgcc acgcaacgct gtcgcgtttc gtcgatcgcg ccggcaatcc
     3421 gatcgacatc ggcatcgcgt tgttttttcc aggaccgctg tcgtacaccg gcgaacacgt
     3481 gctcgagctg caaggtcacg gcggtccgat catcgtcgag cagctcatcg agcgctgtct
     3541 cgagcttggc gcgcgccgcg cgcgtcccgg cgaattcacc gagcgcgcat tccacaacga
     3601 caagatagat cttgcgcagg ccgaagccgt cgcggatctc atcgatgccg gttcacgtga
     3661 agcggcacgt gccgccatgc gttcgctgca aggtgagttc tcgggaatgg tgcagggcct
     3721 taccgactca ctgatcgacc tgcgcacgta cgtcgaagcc gccatcgact ttcccgaaga
     3781 ggaagtcgat ttcctcggcg acaaggaatt gcacgagcgc ctggctaccg tgcgcgctca
     3841 tttcgacgcc gtctccgcgg ccgcgagcca ggggcggctg ctgcgcgatg gcatgaccgt
     3901 cgtgctggcg ggccggccga actccggcaa gtccagcctg ctcaaccggc tcgcgggcta
     3961 cgaggccgca atcgtgaccg ccattccggg aaccacgcgc gacgtgttac gcgagcgcat
     4021 cgacatcgac ggcctgccac tgcacgtgct cgacaccgcc gggttgcgcg actcagacga
     4081 tgtggtcgag cgcgaaggcg tccgacgagc cgcggctgaa atgtcgcgcg cggatcgtgt
     4141 gttgttcgtc atcgacacct cgaccgatcc tcacggccgc gcgttcgaac tcgagcgcga
     4201 gcgtctgccc gcggatgtac ccgtcacgct gatcttcaac aagatcgacc tgggcgaagg
     4261 actgccggtg ggcgatacca tctccggtcc gccgcgcatt catgtctcgg cggttaccgg
     4321 aaagggactg gaccttctgc gcgcgcacct caaagatagc gtcagctttc agaccgcggg
     4381 cagcgggacc gtgtccgcgc gccgccggca tctcgaagca ttggcgcgcg cgcgcgaatg
     4441 cgtcgaaacc gccgcgagca cgctcaacca gcggcgcggt ggcgaactgg ttgccgaaga
     4501 attgcgccag ggccagcagg ccctcgagga aattaccggc atcttttccg ccgacgattt
     4561 gttaggacgt atcttcggct cgttctgcat agggaagtga catgtgggtc ggtaatcgtt
     4621 gttatccccc gacagatgtt caacaggcgc tggagagcct gttcggcgaa tccgtgggcc
     4681 agatcaaggt catccagtac agcatcttcg tgcggtttca ctggcgcgcc atcgcgacga
     4741 cgcgacccaa ccgcatctac ctgcgtggca gtggcaagga attcttcgct gacggcccgc
     4801 tggtgctgca cgaatatttt cacgtcatcc gccagtgggg cacgggcgac atgac
//
LOCUS       DATDEU010000301         5287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_929010, whole genome shotgun sequence.
ACCESSION   DATDEU010000301 DATDEU010000000
VERSION     DATDEU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929010"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIV63_07255"
     CDS             complement(<1..224)
                     /locus_tag="VIV63_07255"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEY6124432.1"
                     /translation="MKISLVAVAAVALVAGCATGPKLDYTDYAGEPVRSFYMNNFDGW
                     TVVSKDQLVVWSGMNKAYLLRITGYCPDLL"
     gene            complement(302..3445)
                     /locus_tag="VIV63_07260"
     CDS             complement(302..3445)
                     /locus_tag="VIV63_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY6124433.1"
                     /translation="MPQFFIDRPVFAWVIAIIITLGGAFAIRSLPAEAYPDVAPPQVS
                     ITATYPGANADVVERTVVQVIEQQLTGIDNLMYFSSSSSNGAGSITLYFESGTNPDTA
                     AVQTQNRVTLAEPRLPREVVAQGLQVAKVNSGFLMAVSLRSKDNSVPSDVLNNIVAAQ
                     VLDPISRIPGVGQTQQFGAEYSMRIWLNPDKLRSFRMSAADVLAKVRGQNVQFATGAL
                     GEQPAIQGQQVVAPVSAEAQFSTKEEFENVILRTEPNGTSVRLKDVARVELGLNSYSF
                     DVRLDNQPVAGFGVLLSPGANALDVARLVKERMNELAPSFPAGVEWFLPFDATIFINA
                     AIHEVVVTLLIAVVLVFIVMLVFLQSFRATLIPTLVVPVALMGAFIGMAVFGFSINQL
                     TLFGMVLAIGIVVDDAIVVIESVERIMREEHLSPKEATRKAMTQITNPIIAISVVLAA
                     VFIPSALQTGSVGMIYQQFAMTIAISMGFSAFLALSLTPALCGTLLRPEHLKENRFFA
                     LFNRGYGKTQDSYIRSVKYSLRHQRFWLAGFAVLLLVGGYTFTQVKGSFVPEEDQGFT
                     IGMVLMPPGTSQPRTREFMRKVSEQLRTHEAVDSVFEVTGFSFIGSGEGVGMFFIKLK
                     DYGDRKATATEFNGFASGLGFSQDDGIGIFVNFPTIPGLGNFGGFEFWLEDRKSAGRP
                     ALYGAMGALLGKAGESPVLAGVQPNELPPAPQLKLSLDREQAESMGLAIGDVYAAVQL
                     MLAPVYVNDFLFEGRVLRVTMQADAPFRMNEDSLQRFYLPAGTTSTTNSFAFAGDSAS
                     DGMVPLSSVLRSNWTVAAPSQARFNGFPAIQLNGGPKEGKSSGEAMTEMERIVREDLP
                     PGFGYDWAGQSFQERLSGAEAPMLFALSILVVFLCLAALYESWATPIAVLLVVPVGII
                     GAVFAAKLAGMPNDVFFKVGLITIIGLAAKNAILIVEFALEEQKRGRPLYESVTEAAR
                     LRLRPILMTSFAFILGVLPLVFATGAGANARRALGTGVVGGMLSAAILGVLLAPVFYV
                     AIRRMVGDKLTLPVKPD"
     gene            complement(3450..4622)
                     /locus_tag="VIV63_07265"
     CDS             complement(3450..4622)
                     /locus_tag="VIV63_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6124434.1"
                     /translation="MLSRVQLISAAAAALFVAACSKQEAPQQPPPPEVGVVVVQGGPV
                     PLVREASGRLAPTRASDVRARVPGVLQKRIYKEGSMVKEGDPLVIIDTAPYRAALAQA
                     NAALEQAEASATNAKVTADRNRELAKQNLVSRMQLDDSQALERSSAAQVSAARAQVQT
                     ARINLGYATITAPISGRASQMRVLEGALVGQGEATLLTTIEQVDPIYVFFDQPASDFE
                     RLQRAQASGAVTLAQGNLAEVRLLRADGSLYDEKGTLDFSDYSVNPTTGAVAFRGKVA
                     NPGRVLLPGMYVNVRLSVGTLNKGYRVPQLAVQRDGQGPYVLVVGADGMVAQKRVQTA
                     SVVDNDWVISDGLADGDQVIVNNLQKIGPGMPVKAKPAGAAEPEKPAAAPAPAAAG"
     gene            complement(4683..>5287)
                     /locus_tag="VIV63_07270"
     CDS             complement(4683..>5287)
                     /locus_tag="VIV63_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEY6124435.1"
                     /translation="LEYAVEARWVTDESSALEWIGGIGDASLGTLDLPVLARLLAAWK
                     QGDHDSVLRWNAFLIACRETEELRAEDRHLGAALLRVLVELQLTTGLFSANAAKSVVG
                     VSHATAFAFAGARWNFDPEACLQTYAWAWIENQVLAAVKLIPLGQSAAQRMLHALIAR
                     IPPLVERAQKLADSDIGISTALSAVASGRHEAQYSRLFRS"
BASE COUNT          974 a         1696 c         1706 g          911 t
ORIGIN      
        1 tacaggaggt ccgggcaata gccggtgatc cggagcagat acgccttgtt catgccggac
       61 cagacgacga gttgatcctt ggagaccacc gtccagccgt cgaagttgtt catgtagaac
      121 gatctgaccg gctcgcccgc gtaatcggtg taatccagct tgggcccggt ggcgcagcct
      181 gcgaccagag ccacggccgc cacggcaacc agtgaaatct tcataaacac ctcttgcgaa
      241 ttacgactca cctgtcgtga gtggagttcc ccgggcgctc ggttcctgaa gacatgtcga
      301 ttcagtcggg tttgaccggc aacgtcagct tgtcgcccac catgcgccgg atggccacat
      361 agaagacggg cgccagcaac acgccaagaa tcgccgccga caacatgccg ccgacgacgc
      421 ccgttccgag tgcgcggcgc gcattggcgc cggcgccggt cgcgaacacc agcggcaaca
      481 cgcccaatat gaaagcgaac gaggtcatca ggatcggtcg aagtcgcaga cgcgcggcct
      541 cggtgacgga ctcgtacaac ggcctgcccc gtttctgttc ttcgagcgcg aactcgacga
      601 tcaggatggc gttcttcgcc gcgaggccaa tgatggtgat gagtcccacc ttgaagaaca
      661 cgtcgttggg catgccggcc agcttcgcgg cgaacaccgc gccgatgatg cccacgggca
      721 ccaccagcaa caccgcgatg ggtgtcgccc agctttcata tagcgccgcg aggcacagga
      781 acacgacgag gatggacaac gcgaacagca tcggcgcctc ggcgccggac agccgttcct
      841 ggaaggactg ccctgcccag tcatatccga agcccggcgg cagatcctcg cgcacgatgc
      901 gctccatctc cgtcatggcc tcgccagaac tcttgccctc tttgggtccg ccattgagct
      961 gaatggccgg aaatccgttg aaacgcgcct gtgaaggcgc cgccacggtc cagttggaac
     1021 gcagcaccga agacagcggc accatgccgt ccgacgcgga gtcgcctgca aaggcgaagc
     1081 tgttggtagt cgaggtggtg ccggccggta gatagaagcg ctgcagcgag tcttcgttca
     1141 tgcggaacgg cgcgtcggcc tgcatggtca cgcgcaatac gcggccttcg aacaggaagt
     1201 cgttcacgta caccggggcg agcatgagct gcacggcggc atacacgtcg ccgatcgcaa
     1261 gtcccatcga ttccgcctgc tcgcgatcca gtgacagttt cagctgcggc gcgggcggca
     1321 gttcgttcgg ctgcacaccg gcgaggaccg ggctttcccc ggccttgccg agcagagctc
     1381 ccatggcgcc atacaacgcc ggccgtcccg cggatttacg gtcctcgagc cagaactcga
     1441 agccgccgaa attgccgagt ccggggatcg tggggaaatt gacgaagatg ccgatgccat
     1501 cgtcctggct gaatcccaat ccggaagcga aaccgttgaa ctcggtggcc gtggcttttc
     1561 gatcgccata gtccttgagc ttgatgaaga acatgcccac gccttcaccg ctgccgatga
     1621 agctgaagcc ggtgacttcg aagaccgagt cgaccgcttc gtgcgtgcgc agttgttcgc
     1681 tgaccttgcg catgaactcg cgcgtgcgcg gctggctcgt gccgggcggc atcagcacca
     1741 tgccgatggt gaagccctgg tcttcttcgg gcacgaagct gcccttcacc tgcgtgaacg
     1801 tgtagccgcc cacgagcagc aggaccgcga accccgcgag ccagaagcgc tgatggcgca
     1861 ggctgtattt caccgagcgg atgtacgagt cctgagtctt gccgtagccg cggttgaaca
     1921 gcgcgaagaa acggttttcc ttgaggtgct cgggacgcag cagcgtgccg cacagcgccg
     1981 gcgtaagcga cagcgcgagg aacgcggaga agcccatgga gatcgcgatg gtcatcgcga
     2041 actgctggta gatcattccc accgagccgg tctgcaacgc gctcgggatg aacaccgccg
     2101 cgagcaccac cgagatcgcg atgatcgggt tggtgatctg cgtcatcgcc ttgcgcgtgg
     2161 cttccttggg cgagagatgt tcctcgcgca tgatgcgttc gacggattcg atgaccacga
     2221 tggcgtcgtc gaccacgatg ccgatggcca gcaccatgcc gaacaaggtg agctggttga
     2281 tcgagaatcc gaacacggcc atgccgatga acgcgcccat gagcgccacc ggcaccacca
     2341 gcgtgggaat gagcgtggcg cggaaactct gcaggaacac cagcatcacg atgaacacga
     2401 gcacgacggc gatcagcagc gtgaccacca cttcgtgaat ggcggcgttg atgaagatgg
     2461 tcgcatcgaa cggcaggaac cactcgacgc cggccgggaa gctgggcgcg agttcattca
     2521 tgcgctcctt gacgagccgc gccacgtcga gcgcatttgc gccgggtgac agcaacacgc
     2581 cgaagccggc gacgggctgg ttgtcgagcc gcacgtcgaa cgaatagctg ttgaggccga
     2641 gctcgactcg cgccacgtct ttcaatcgca cgctggtgcc attgggctcg gtgcgcagga
     2701 tgacgttctc gaactcttcc ttggtgctga attgcgcctc ggcggagacc ggcgccacga
     2761 cctgctgacc ctggatcgcc ggctgttcgc cgagcgcgcc cgtggcgaac tgcacgttct
     2821 ggccacgcac tttcgccagc acatcggcgg cgctcatgcg gaagctgcgc agcttgtccg
     2881 ggttcagcca gatgcgcatc gaatattcgg cgccgaactg ctgtgtctgg ccgacgccgg
     2941 gaattcgcga gatcgggtcg aggacctgcg ccgcgacgat gttgttgagc acgtcggaag
     3001 gcacgctgtt gtccttcgaa cgcagactca ccgccatgag gaagccggag ttcaccttcg
     3061 cgacctgcag gccctgcgcc accacttcgc gcggcaggcg cggctcggcc agcgtcacgc
     3121 ggttctgcgt ctgcacggcc gccgtatcgg ggttggtccc cgattcaaaa taaagagtga
     3181 tgctgccggc gccgttggac gacgacgacg agaaatacat gaggttgtcg atgccggtga
     3241 gctgctgctc gatgacctgc acgaccgtgc gctcgaccac gtcggcattg gcaccgggat
     3301 aggtggcggt gatcgacacc tgcggtggcg cgacgtccgg atacgcctcg gccggcagcg
     3361 agcgaatggc gaacgcacca cccagggtga tgatgatggc gatcacccac gcgaataccg
     3421 ggcggtcaat gaagaattgc ggcatgggcc taaccagcgg cggcgggagc gggagccgcg
     3481 gctggcttct cgggctcggc agcgccagcc ggtttcgcct tgaccggcat ccccggccct
     3541 atcttctgca ggttgttgac gatcacctgg tcgccatcgg cgaggccatc ggagatcacc
     3601 cagtcgttgt cgaccaccga cgcggtctgc acacgcttct gcgcgaccat gccgtccgcg
     3661 cccacgacga ggacgtaagg accctgccca tcgcgctgca cggcgagctg cggcacgcga
     3721 taacccttgt tcagcgtacc gacgctcagc cgcacgttca cgtacatgcc cggcagcagc
     3781 acgcgtcccg gattcgccac cttgccgcgg aacgccacgg cgcccgtggt cgggttcacg
     3841 ctgtagtcgg agaaatcgag cgtgcctttc tcgtcgtaga gcgaaccgtc ggcacgcagc
     3901 aggcgcacct cggcgaggtt gccctgggcc agcgtgacgg cgcccgaggc ctgcgcgcgc
     3961 tgcaggcgtt cgaagtcgga ggccggctgg tcgaagaaca cgtagatggg atcgacctgc
     4021 tcgatggtag ttagcagcgt cgcttcgccc tgccccacga gcgcgccttc gagcacgcgc
     4081 atctggctcg cgcgtccgga gatcggcgca gtgatggtcg cgtatccgag attgatgcgt
     4141 gcggtctgca cctgggcacg cgcggcggag acctgcgcgg ccgaggaacg ctcgagagcc
     4201 tgcgagtcgt cgagctgcat gcgcgacacc aggttctgtt tcgcgagttc gcggttgcgg
     4261 tccgcggtga ccttcgcatt ggtggctgac gcttcggctt gctcgagcgc cgcgttggcc
     4321 tgcgcaagcg cggcgcgata cggcgccgtg tcgatgatca cgaggggatc gccctccttg
     4381 accatgctcc cttccttgta gatgcgcttc tggagcacac ccggaacgcg cgcgcgtacg
     4441 tcggaggccc gcgtgggtgc gagtcgccct gaggcttcgc gcacgagcgg cacaggcccg
     4501 ccctggacta cgacaactcc gacttcaggc ggaggcggtt gctgcggcgc ctcctgcttg
     4561 ctgcaagcgg cgacaaacaa ggcggcagca gccgcggata tcagctgaac gcgtgaaagc
     4621 attttgtttt tgggcgcggc gggggttgga atgtccaccg gctaggcggc ggattctagc
     4681 ccttacgatc gaaacagtcg cgagtactgc gcttcgtgac ggccgctggc gaccgcgctc
     4741 aacgcggtgg atatcccgat gtcggaatct gcgagttttt gtgcgcgctc taccaaagga
     4801 gggatgcgag cgatgagcgc atgcagcatc ctttgcgcag cgctttgccc caatggaatg
     4861 agcttcacgg ccgcgagaac ctggttctcg atccacgccc aggcgtaggt ttgcagacac
     4921 gcttccgggt cgaaattcca ccgcgctccc gcgaaggcaa acgcggtcgc atgcgacacg
     4981 ccgacgacac tcttggcggc gttcgccgaa aacaatccgg tcgtaagttg cagctcgacg
     5041 agcacacgca acagcgcggc gcccagatgc cggtcttcgg cacgcaactc ttcggtttcg
     5101 cgacaggcga tcaggaacgc attccatcgc agcacgctgt cgtgatcacc ctgtttccac
     5161 gcggccagca ggcgcgcaag caccgggagg tcgagcgtgc ccaggcttgc gtcgccgatg
     5221 ccgcctatcc actcgagcgc gctcgattcg tcggtcaccc agcgtgcttc gaccgcgtac
     5281 tcgaggc
//
LOCUS       DATDEU010000302         5107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_637536, whole genome shotgun sequence.
ACCESSION   DATDEU010000302 DATDEU010000000
VERSION     DATDEU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637536"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VIV63_07275"
     CDS             <1..496
                     /locus_tag="VIV63_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6124436.1"
                     /translation="VILNFARAPIVDEKAIIAALDDARLQGYVCDFPTNALKDHPKVV
                     TLPHLGASTNEAEDNCAVMVADTVKDYLENGHIKNSVNFPEVVLPRAPKTARICIANS
                     NVPNMVGQISTVLAGASLNIAELLNKSRGEYAYTVIDTDGAVDPGVIDKVRAISGVLA
                     ARVI"
     gene            544..1638
                     /gene="pheA"
                     /locus_tag="VIV63_07280"
     CDS             544..1638
                     /gene="pheA"
                     /locus_tag="VIV63_07280"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY6124437.1"
                     /translation="MAKVADPLAGLRAQIDGVDARIHALLNDRAALARQAGVSKHADG
                     HTVDYYRPEREAQVLRMARERNKGPLRTEEILRLFREIMSACLAQEEPLKVGYLGPEG
                     TFSQTATLKHFGHSVRALALASVNEVFHEVEAGLADFGVVPVENSTEGAVNHTLDTLL
                     GSPLKICGEVELRIHQFLMGGMDNLAKIKRICSHPQSLAQCRGWLDEHLPDLPRIPLS
                     SNAEAARRARDEEGTAAIAGETASDVYGLKLLATEIEDSDDNTTRFFVVGRKLFKPSG
                     DDRTTILCSALDTESPGALFRLLEPLAKHKISLTRIESRPSRRKKWDYVFFFDIVGHA
                     EEVHVAKALTALKKRASLFRVLGSYPRAVS"
     gene            complement(1664..2683)
                     /locus_tag="VIV63_07285"
     CDS             complement(1664..2683)
                     /locus_tag="VIV63_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY6124438.1"
                     /translation="MRSRDLSFAWGCVLLLAGCSHDRLRPETAQCAVDANQEKALWYM
                     GNASNVRSGALVFTKLQDDKSMIEVWRGCNQTQRKTVALTVSNVGHLAVSESGRYAVA
                     SGSAAGVEVVDLESGKAIAHVPDGLGPGLFSPDERFVLFNTWGAGVRIFRVGIDEITP
                     MANYKTLGSVYAMSADYPFKRLAIGDVEGKIQLLDIRETAAGLELINPRVIDEGNRAR
                     ISGFGFSPTSDALYVLSGDGRLTVWDVKSGAKRHSWKTGLKWVGSVTFLRGTPRAVVF
                     GTVEPSGLAGPLGIVIDTESGRTRSFVTRQNYLWGAYIPRSDELFVTGFWQKPYLEKD
                     ISVIP"
     gene            2787..3905
                     /gene="hisC"
                     /locus_tag="VIV63_07290"
     CDS             2787..3905
                     /gene="hisC"
                     /locus_tag="VIV63_07290"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY6124439.1"
                     /translation="MPTNTSDPGLLAVDNVRTLAPYVPGKPIEELQRELGLTDIIKLA
                     SNENPFGASPKALAAMHSAIADTWLYPDGSGHLLKQKLAAKLGVDSAQITLGNGSNDV
                     LVMLAEAFLGPGVEAIYSQYGFAVYPIAVQATGAIAVVTPALPADSPMPLGHDLDAMA
                     RAITPRTRVVFIANPNNPTGTWVPAAPLKAFIAAVPGHVLVALDEAYFEYTGGLDLQD
                     GVEWLREFPNLVVFRTFSKAYGLAGVRVGYAVSHPSVAGMLNRVRQAFNVSVVGLAGA
                     AAALDDTEHLANAVRVAVAERTRVTTLLQGGGTRVLPSAGNFLMLHAGPNAKDRFEAL
                     LRRGIIVRPVGNYGLSEHLRVTLGTPEQNDRFLKAWEA"
     gene            3921..4616
                     /gene="cmk"
                     /locus_tag="VIV63_07295"
     CDS             3921..4616
                     /gene="cmk"
                     /locus_tag="VIV63_07295"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY6124440.1"
                     /translation="MSPDTSSVPIVTIDGPSSSGKGTISRIVASRVGWNLLDSGALYR
                     LVALGGILKDLDPDDVEEHVSVARSMRVEFGSVGEGQEQVLLDGRDVTQKIRTEQAGA
                     GASRVAAWPAVRTALTDRQKSFAKPPGLVADGRDMGTVIFPEARLKVFLTASAEERAQ
                     RRHKQLIGKGSAASLAALSREIAERDLRDSTRQVAPLKPAPDAQMLDSSGLSIEAVVE
                     RVISLGRERNLWR"
     gene            4745..>5107
                     /locus_tag="VIV63_07300"
     CDS             4745..>5107
                     /locus_tag="VIV63_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY6124441.1"
                     /translation="MTESFAQLFEQSIANQRIRPGMILTGLVVSVGDDMVIVNVGLKS
                     EAVIPLEQFKNERGEVEVKAGDSIEVALDAMEDGTGETRLSREKAKRARTWTRLEESF
                     NKSEIVTGTITGRVKGGFT"
BASE COUNT          997 a         1676 c         1547 g          887 t
ORIGIN      
        1 cgtcatcctg aacttcgcgc gcgcgcccat cgtcgatgag aaagccatca tcgccgcgct
       61 cgacgacgcc cgcctgcagg gctatgtctg cgacttcccg accaacgcgc tcaaggatca
      121 ccccaaggtc gtcacgctgc cgcatctcgg cgcctcgacc aacgaagccg aagacaactg
      181 cgcggtcatg gtggccgaca ccgtcaagga ctacctcgag aacggccaca tcaagaacag
      241 cgtcaacttc ccggaagtgg ttctgccgcg cgcgccgaag accgcgcgca tctgcatcgc
      301 caacagcaac gtgcctaaca tggtcggcca gatctcgacc gtgctcgcgg gcgcctcgct
      361 gaacatcgcc gaactgctca acaaatcccg cggcgaatac gcctacaccg tcatcgatac
      421 cgatggcgcc gtggatcccg gggtcatcga caaggtgcgt gcgatcagcg gcgtgctggc
      481 cgcgcgggtg atataacgac ttcgatggcc aggaagtcga aaagcggacc gaaagcggcg
      541 ggggtggcaa aggttgccga cccgctcgct ggtcttcgcg cgcagatcga tggggtggat
      601 gccaggatcc acgcgctgct caacgatcgc gcggctctcg cacggcaggc cggcgtctcc
      661 aaacacgccg acggccatac ggtggactac taccggcccg aacgcgaagc gcaggtgctg
      721 cgcatggcgc gagagcggaa caagggtccg ctgcgcaccg aagaaatcct gcgcttgttc
      781 cgcgaaatca tgtccgcgtg cctcgcgcag gaagaacccc tcaaggtggg gtacctcggg
      841 cccgagggca cgttctcgca gaccgccaca ctcaagcact tcggccactc ggtgcgcgcg
      901 ctggcgctcg cttcggtcaa cgaagtgttc catgaggtcg aagcggggct cgccgacttc
      961 ggcgtggtgc ccgtggagaa ctccaccgaa ggcgccgtca accatacact cgacaccttg
     1021 ttaggctcgc cgctcaagat ctgcggcgaa gtcgagctgc gcatccacca gttcctcatg
     1081 ggtggcatgg acaacctggc gaagatcaag cgcatctgca gccatccgca gtcgctcgcg
     1141 caatgccgcg gctggctcga cgagcacctg cccgacttac cgcgcatccc gctgtcgagc
     1201 aatgccgaag ccgcgcgccg cgcgcgtgac gaagagggca cggccgccat cgccggtgaa
     1261 acggcgtcgg atgtttacgg cctgaaactg ctcgccaccg aaatcgaaga cagcgacgac
     1321 aacaccacgc ggttttttgt agtaggacgg aagctcttca aaccttccgg agatgaccgc
     1381 accaccatcc tctgttcggc tctcgataca gagtcgccgg gtgcgttgtt ccgtctgctc
     1441 gaaccgctgg ccaagcacaa gatcagcctc acgcgcatcg aatcgcggcc gtcgcgccgc
     1501 aagaagtggg actacgtgtt cttcttcgac atcgtgggcc acgcggaaga agtgcacgtc
     1561 gcgaaggcgc tcaccgcgct caagaagcgt gcctcgctgt tccgcgtgct gggctcatac
     1621 ccgcgcgccg tgtcgtaacc gacaccgacc tgcggttcga gcgtcaggga atgacgctga
     1681 tatctttctc gaggtaaggc ttctgccaga agccggtgac gaacaattcg tcgctgcgag
     1741 ggatatacgc gccccacaga tagttctgac gagtcacgaa cgagcgcgtc cgtccggatt
     1801 cagtgtcgat gacgatgccg agcggacctg caaggcccga gggttcgacg gtgccgaaaa
     1861 ccaccgcgcg cggagtgccc cggagaaagg tcacgctccc gacccatttc aatccagtct
     1921 tccagctatg ccgcttcgcg cctgatttca cgtcccatac ggtgaggcgt ccatcgccag
     1981 aaagcacata gagcgcatcc gatgtgggcg aaaacccaaa tccggatatc cgcgcacgat
     2041 tgccttcgtc gatcacgcgc ggattgatga gttcaaggcc tgccgcggtc tcccggatgt
     2101 ccagcaattg aatcttgccc tcgacatcgc cgatggcgag ccgtttgaac gggtaatcgg
     2161 cggacatcgc atacacggaa cccaacgtct tgtagtttgc catcggcgtg atctcgtcga
     2221 tcccgacacg aaagatccgc actcccgcgc cccacgtatt gaagagaacg aaccgctcgt
     2281 cgggcgaaaa cagtccggga cccaagccat cgggaacgtg agcgatggcc ttgccgcttt
     2341 ccagatccac gacttcgacg ccggcagcgc tgccgctcgc cactgcgtag cgtccgcttt
     2401 cggacaccgc gagatggccc acgttcgaga ccgtgagggc caccgttttc cgctgcgtct
     2461 ggttgcagcc gcgccagacc tcgatcatcg atttatcgtc ctggagtttc gtgaatacca
     2521 gcgctccgga ccggacgttc gacgcgtttc ccatgtacca aagcgctttt tcctggttgg
     2581 catcgaccgc gcactgtgcg gtttccggcc gaagccgatc gtggctgcaa ccggcgagca
     2641 gaagcacgca accccaggcg aaactcagat ctcttgaccg catggcgcgc gcttttgcta
     2701 gtgaggacac ctttaattga agcccgggat tctggcaggc gtgtggcgcc aagcaggcga
     2761 atacggaatt cactaggatt cgggccatgc ctacgaatac atcggatccg ggtttactgg
     2821 ccgtcgacaa cgtccgcacg ctcgcgccct atgtgcccgg caagcccatc gaagaattgc
     2881 aacgtgagct cggactcacg gacatcatca aactcgcgtc gaacgagaat ccgttcggcg
     2941 cgagcccgaa ggcgctggcc gccatgcaca gcgccatcgc cgacacctgg ctgtatcccg
     3001 acggcagcgg acacctgctc aagcagaagc tcgcggcgaa actcggcgtc gacagcgcgc
     3061 agatcacgct cggcaacgga tccaacgacg tgctggtgat gctcgccgaa gccttcctgg
     3121 gtcccggcgt cgaagctatc tactcgcagt atggtttcgc cgtgtatccc atcgccgtgc
     3181 aggccaccgg cgccatcgcg gtcgtcacac cggcattacc cgccgattcg cccatgccgc
     3241 tcggccacga tctcgacgcg atggcacgcg ccatcacgcc gcgcacgcgc gtggtgttca
     3301 tcgccaaccc taacaacccc acgggaacct gggtgccggc cgcgccgctc aaggctttca
     3361 tcgccgccgt gccgggtcac gtgctggtcg cgctcgacga agcgtatttc gaatacaccg
     3421 gcggactgga cctgcaggac ggcgtcgaat ggttgcgcga gtttccgaac ctcgtggtgt
     3481 tccgcacgtt ttcgaaagcc tacggtctcg ccggcgtgcg cgtgggttac gcggtgagcc
     3541 acccgtcggt tgccggcatg ctcaatcgcg tgcgccaggc attcaacgtg tcggtggtgg
     3601 gcctggcggg cgccgcggcg gcactggatg acaccgaaca cctcgccaac gccgtgcgtg
     3661 tcgcggtcgc ggaacgaacg cgcgtgacga ccctcctgca aggcggtggc acccgcgtgt
     3721 tgccctcggc cggcaatttc ctgatgttgc atgccggtcc caacgccaag gatcgattcg
     3781 aggcattgct ccgacgcggc atcatcgtgc ggccggtggg caactatggc ttgtccgaac
     3841 acctgcgcgt cacgctcggc actccggagc agaacgatcg tttcctgaaa gcctgggaag
     3901 cttgatagcc tctcgggcca atgagcccag acacatcttc tgttcccatc gtcaccatcg
     3961 acggtccctc gagttccggt aaaggcacca tcagccgaat cgtcgcttca cgcgtcggct
     4021 ggaacctgct cgacagcggc gccctgtatc gactcgtcgc gctgggcggc attctcaaag
     4081 acctcgaccc cgacgacgtg gaagaacacg tgtccgtggc ccgttccatg cgcgtggagt
     4141 tcggctccgt gggcgaaggg caggagcagg tgctgctcga cggtcgggac gtcacccaga
     4201 agatccgcac ggagcaggcc ggggcgggcg cctcccgggt agccgcgtgg ccggcggtcc
     4261 ggacggccct gaccgaccgg caaaagtcct tcgccaaacc cccggggttg gtcgccgacg
     4321 gccgggatat gggcacggtc atctttccgg aggcccggct gaaggttttc ctcacggcga
     4381 gcgccgagga aagggcccaa aggcgtcata agcagttgat cggcaagggc tccgctgcta
     4441 gtcttgccgc cctttcgcgg gagatcgcgg agcgcgattt gcgggattcc acccgccagg
     4501 tcgctccgct caaacccgcg cccgacgcac agatgctcga ctcctccggg ttgagcatcg
     4561 aagcagtcgt cgagcgagtc atctcgctcg ggcgcgagcg caatctctgg cgttgaggtt
     4621 tctaggttgc tctgcaagga cgcggagctg tgtgtagcta ccttggggga agaggatttc
     4681 ccgcgagcgt caattgtcac ggcacctcac tcaaaaaaat ggtgaccgtg cgaatggaaa
     4741 ctaaatgact gaaagttttg cccagctctt cgaacagagc atcgctaacc agcgcatccg
     4801 ccccggaatg atccttacag gcctcgtggt cagtgtcggc gacgacatgg tgatcgtgaa
     4861 cgtgggtctg aagtccgaag ccgtcatccc cctcgagcag ttcaagaacg agcgcgggga
     4921 agtcgaagtc aaagccggcg acagcatcga agtcgcgctc gacgcaatgg aagacggcac
     4981 tggcgaaacg cgcctctcgc gcgaaaaagc caagcgtgcc cgcacctgga cgcgtctcga
     5041 agagtctttc aacaaatccg aaatcgtcac cggcaccatc acgggccgtg tgaagggtgg
     5101 cttcacc
//
LOCUS       DATDEU010000303         3642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_53726, whole genome shotgun sequence.
ACCESSION   DATDEU010000303 DATDEU010000000
VERSION     DATDEU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53726"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..2920
                     /gene="uvrA"
                     /locus_tag="VIV63_07305"
     CDS             77..2920
                     /gene="uvrA"
                     /locus_tag="VIV63_07305"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY6124442.1"
                     /translation="MTTQQIRIRGARTHNLKNISVDLPRDKLIVITGLSGSGKSSLAV
                     DTLYAEGQRRYVESLSAYARQFLSMMDKPDVDSIEGLSPAIAIEQKATSHNPRSTVGT
                     VTEIYDYLRLLFARAGIARCPDHGIDLAAQTVSQMVDQVLRMPDGLAVLLLAPIVIDR
                     KGEHAEVFEQLRGQGFVRVRIDGRVHELDALPKIDAKKKHTIDVVVDRLRIRADAAQR
                     LSESLETALKLAGGTARLAPYEAEKVSRPEDETLFSARQACPKCGYSVPALEPKMFSF
                     NSPAGACPTCDGIGLKDFFDPLRVVAYPHLSLAGGAIKSWDRKNAYYFALITAMAKHY
                     KFDPETPWENLSKKAQQVMLFGSGNDEVEFRYVHGNGRATKKKHRFEGIIPNLERRYK
                     ETDSLPVREELAKYLGSQPCPDCHGTRLNRTARNVFVGTHSLPEIAHLTVGDAKAMFG
                     SLDVAGWRAEVADKIVKEVGDRLKFLVDVGLDYLTLDRSAESLSGGEAQRIRLASQVG
                     SGLTGVMYILDEPSIGLHQRDNQRLLATLKHLRDLGNTVLVVEHDEEAIRAADAVLDI
                     GPGAGVHGGQIVAQGTPAEIEAHPDSLTGKYLSGRTKIALPGLRTPRDPKRVIKVVGA
                     TGNNLKKVDAEFPLGLFTCVTGVSGSGKSTLVNDTLFTHANHKLNGTVMDMAPCQEIK
                     GLEAIDRIIDIDQSPIGRTPRSNPATYTGLFGPLRELFAEVPESRTRGYDAGRFSFNV
                     KGGRCEACQGDGLIKVEMHFLPDVYVPCDVCQGKRYNRETLDIRWRGKNIHEVLDMNV
                     EDALRLFQNVPGVGTRLQTLMDVGLSYLQLGQSATTLSGGEAQRVKLARELAKRATGR
                     TLYILDEPTTGLHFHDVAQLLDVLHRLRDEGNTVVVIEHNLDVIKTADWIIDLGPEGG
                     AGGGNILATGTPEEVARHPKSYTAQYLRPLVGS"
     gene            complement(2917..>3642)
                     /locus_tag="VIV63_07310"
     CDS             complement(2917..>3642)
                     /locus_tag="VIV63_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124443.1"
                     /translation="STWTTDSNMDGEDDSRTTWDVEYDTNHRPVTERYETDDDADGVI
                     DSRETNTFMPAANGRDVSEVRLSDLDADGIVEIRTQTDWVLNSTGGTRTVSVEVEYDF
                     DEDEVVESRYRDVSTLDADGRIVDTVVSEDYDGDGVVNSVTHRTNTYDGYGNLLVAVS
                     ETDSGNDGQWETRAITQYEYGAGAELLGAVRTLHEMGGPGVPYGTIRATNLLLANGVL
                     MLAQRYLGGISFGGAVATDAIVN"
BASE COUNT          709 a         1178 c         1117 g          638 t
ORIGIN      
        1 caggtactgg ttatgcatga gccggcctcg ccgcttagac tccgcggttt ccattcattc
       61 gcgcgagaaa ttcccgttga ccacgcaaca gatccggatt cgcggtgccc gcacacacaa
      121 cctcaagaac atcagcgtcg acctgccgcg cgacaaactc atcgtgatca ccgggctgtc
      181 gggatccgga aaatcctcgc tggcagtcga cacgctgtac gcggaaggcc agcgccgtta
      241 cgtcgaatcg ctgtcggcct acgcgcggca attcctgtcg atgatggaca agcccgacgt
      301 cgacagcatc gaaggcctgt cgccggccat cgcgatcgag cagaaggcca cctcgcacaa
      361 tccgcgctcg acggtcggca ccgtcacgga aatctacgac tatctacgcc tgctgttcgc
      421 gcgcgcgggt attgcgcgct gcccggacca cggcatcgat ctcgcggcgc agaccgtcag
      481 ccagatggtc gaccaggttc tcaggatgcc cgatggcctc gccgtgttgc tgctggcgcc
      541 catcgtcatc gaccgcaagg gcgaacacgc tgaagtgttc gagcagctcc gtggccaggg
      601 cttcgtgcgc gtgcgaatcg acggccgtgt ccacgagctc gatgcgctgc cgaagatcga
      661 cgcgaagaag aaacacacca tagacgtggt ggtagataga ttgcgcatcc gcgccgacgc
      721 ggcgcagcgc ctgtcggaat cgctggagac ggcgctgaag ctggctggcg gcacggcccg
      781 cctcgcgccc tacgaagcgg agaaggtttc aaggcccgag gacgagacgc tgttctccgc
      841 gcgccaggcc tgcccgaagt gcggctacag cgttccggcg ctcgagccga agatgttttc
      901 gttcaacagc ccggccggcg cctgcccgac ctgcgacggc atcggcctca aggatttctt
      961 cgatccgttg cgcgtcgtgg cctatcccca tctgtccctg gcgggaggcg cgatcaagag
     1021 ctgggatcgc aagaacgcgt attacttcgc attgatcacg gccatggcga agcactacaa
     1081 gttcgacccg gaaacgccgt gggaaaacct ctcgaaaaag gcgcaacagg tcatgttgtt
     1141 cggcagcggg aacgacgaag tggaattccg ctacgtccac ggcaacgggc gcgccacgaa
     1201 aaagaaacac cgcttcgaag gcatcattcc gaatctcgaa cggcgataca aagaaaccga
     1261 ttcgctgccg gtgcgcgagg aactcgcgaa gtatctcggc agccagccct gccccgattg
     1321 ccacggcacg cggctgaatc gcacggcgcg caacgtgttc gtcggcaccc acagcctgcc
     1381 ggaaatcgcg cacctcacag tgggcgatgc gaaggcgatg ttcggctcgc tggacgttgc
     1441 cggctggcgc gcggaagtgg ctgacaagat cgtcaaggaa gtgggcgatc gcctgaagtt
     1501 cctggtcgac gtgggtctcg actacctcac gctggatcgc agcgcagaat cgttgtcggg
     1561 cggcgaagcg cagcgcatcc gcctcgcgag ccaggtgggc tcgggcctca cgggcgtcat
     1621 gtatatcctc gatgagccgt cgatcggcct tcaccagcgg gacaaccagc ggttgttagc
     1681 cacgctcaaa cacctgcgcg acctcgggaa cacggtgctc gtcgtggagc atgacgaaga
     1741 agcgatccgc gccgccgatg ccgtgctcga cattggcccc ggcgccggcg tgcacggcgg
     1801 ccagatcgtc gcgcagggga cgccggctga aatcgaagcg catccggatt cgctcacggg
     1861 caagtatctt tccggccgca cgaagatcgc gctgccgggg ctgcgtacgc cgcgcgatcc
     1921 caagcgcgtg atcaaggtcg tgggcgcgac gggcaacaat ctcaagaaag tcgacgcgga
     1981 atttccgctg gggctgttta cctgtgtcac cggcgtgtcc ggctcgggca agtccacgct
     2041 ggtcaacgac acgttgttca cgcacgccaa tcacaagctg aacggcacgg tgatggacat
     2101 ggcgccctgc caggagatca agggcctcga agccatcgat cgcatcatcg acatcgacca
     2161 gagtccgatc ggccgcacgc cgcgctccaa cccggcgaca tacaccggct tgtttggacc
     2221 gctgcgcgag ttgttcgccg aagtgccgga gtcgcgcacg cgcggctatg acgccggccg
     2281 cttcagcttc aatgtgaaag gcggccgctg cgaggcctgc cagggcgatg gcctcatcaa
     2341 ggtcgaaatg cacttcctgc ccgatgtgta cgtgccctgc gacgtgtgcc agggcaaacg
     2401 ctacaaccgc gaaacgctcg acatccgctg gcgcggcaag aacatccacg aagttctcga
     2461 catgaacgtc gaggacgcgc tgcgtctgtt ccagaacgtg cccggcgtgg gcacacggct
     2521 gcaaaccctc atggatgtgg gtctgtccta tctacaactg ggtcagagcg ccaccacgct
     2581 ttccggcggc gaagcccagc gcgtcaaact ggcccgggaa ctggccaagc gggcgacggg
     2641 ccgcactcta tatatattgg acgaacccac caccggcctt cacttccacg acgtggccca
     2701 gttgctcgat gtgctgcacc ggttgcgcga cgagggcaac acggtggtgg tcatcgaaca
     2761 caatctcgat gtgatcaaga ccgccgactg gatcatcgat ctcggccccg aagggggcgc
     2821 cggcggcggc aacatcctcg cgacgggcac gcccgaggaa gtggcgagac acccgaagtc
     2881 gtataccgcg caatacctgc ggccgctcgt aggtagttag ttaactatcg cgtcggttgc
     2941 gaccgctccg ccgaagctga ttcctcccag gtaccgctgg gcaagcatca gcacgccatt
     3001 cgcgagcagc agattggtgg cgcgaatcgt tccatagggc actcccggtc cgcccatctc
     3061 atgcaaggtc ctgaccgcgc ccagcagctc cgctccggca ccgtactcat actgcgtgat
     3121 ggcgcgggtc tcccattggc cgtcattgcc cgaatcagtc tcggagacgg cgacgagaag
     3181 attcccgtag ccgtcgtaag tattcgtacg gtgggtgacc gagttcacga cgccatcgcc
     3241 atcgtagtct tcggaaacaa cggtatcgac aatgcgaccg tcggcgtcca gcgtcgacac
     3301 atcgcgatat cgcgattcca ccacttcgtc ttcgtcgaaa tcgtattcga cctctaccga
     3361 gaccgtgcga gtgccgccag tggaattcag gacccagtca gtttgcgtgc ggatctcgac
     3421 gatgccgtcg gcgtccagat cgctgagacg gacttcgctg acgtcgcgtc cgttggcggc
     3481 aggcatgaac gtgttggtct cccgcgaatc gatgacgcca tccgcgtcat catcggtctc
     3541 atatcgctcc gtcaccggtc gatggttcgt gtcgtattcg acgtcccagg tcgttcgcga
     3601 gtcgtcctcg ccgtccatgt tcgaatccgt cgtccaggtg ct
//
LOCUS       DATDEU010000304         2017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1075945, whole genome shotgun sequence.
ACCESSION   DATDEU010000304 DATDEU010000000
VERSION     DATDEU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075945"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VIV63_07315"
     CDS             complement(<1..668)
                     /locus_tag="VIV63_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY6124444.1"
                     /translation="MSGSINLTQSLVQKLGSSIVRGELPAGKPLPIEAELGKKFGASR
                     TVMREAVKILSTKGLIGQRPRVGTYVHPEEKWDLLDAEVLKWILDRHFSHRLVREFLE
                     VRIGIEPAAAALAATNATEEDKELLRLKLEKMKAANEGHFDPVSADIAFHATILEISH
                     NRFFHQLTPIIETALRFSIRLTNKVKGTLPDYDAHEKIYRAIRNGNPEAAFRSCRELI
                     KEAL"
     gene            complement(880..1611)
                     /locus_tag="VIV63_07320"
     CDS             complement(880..1611)
                     /locus_tag="VIV63_07320"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6124445.1"
                     /translation="MQNVRKGFRNEKVNLQVIEGLDFEVREGEFVAIVGPSGCGKTTL
                     MNIMAGFLSPDSGAVMVDGKPRPEPNAKGILITQQGSVFPWLTVKQNLLFGLPDELPN
                     RDALADEYAALVGLKGFENNYPHELSGGMLKRAEIARALVVKPEILYMDEPFSALDAL
                     MSQRMQNELLRILSIEKHTVMLITHDVEEAIHLADRILVLSPRPARIQASFDVPFAHP
                     RRVSSPEAQALRVAILKELGVTETV"
     gene            complement(1634..>2017)
                     /locus_tag="VIV63_07325"
     CDS             complement(1634..>2017)
                     /locus_tag="VIV63_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6124446.1"
                     /translation="FIASVFPMIVQTTSGVHTIEKRYLRAADNFGVSRKKLFLQVVFP
                     ATLPQIIVGMRIGLGVAWLVVVAAEMIALRSGLGYMIIDSRNAGNRYDLVIAGMIIIG
                     LIGLLLDGLMRLLESMKVVRWRYAH"
BASE COUNT          373 a          613 c          626 g          405 t
ORIGIN      
        1 atgagcgctt ccttgatgag ctcgcggcac gagcggaaag cggcttccgg attaccgttg
       61 cggatggcgc ggtagatttt ttcgtgcgcg tcgtaatcgg gcaacgttcc cttcaccttg
      121 ttggtcagac gaatggagaa acgcagcgcc gtttcgatga tgggcgtgag ctggtgaaag
      181 aagcggttgt ggctgatctc gaggatggtg gcgtgaaacg cgatatcggc cgacaccgga
      241 tcgaaatgcc cttcgttggc cgccttcatt ttctcgagct tcagccgaag cagttccttg
      301 tcttcttccg tggcattggt ggctgccaac gccgcggccg ccggctcgat gccgatgcgg
      361 acctcgagga attcacgcac cagccggtgg gagaagtgac ggtcgagaat ccatttgagg
      421 acctcggcat ccagcaggtc ccatttttct tccgggtgca cgtacgtgcc cacgcgcggc
      481 cgctggccga tgagcccctt ggtggacagg atcttcaccg cttcgcgcat caccgtgcgg
      541 ctcgcgccga atttcttgcc gagttccgct tcgatgggca gcggctttcc ggcgggcagt
      601 tcgccgcgga cgatggacga tcccagcttc tgcacgagcg actgggtcag gttgatgctt
      661 ccggacatga gcccgattac ctctctggat ttcgcgcggc cgtccctgac cggatcgtcg
      721 atacgctatc acgagagcgg cccggttttg attattaatc ggactattcg cggaaagggc
      781 gggaatgaag cgattcggcc acctcgggac agcggatgtc tcgtcaggac aggcgccgcc
      841 gggtggcggc cgggcgcgcg cgcccggtag ttaggccgtt cacaccgttt cagtgacgcc
      901 cagttccttg agtatcgcca cgcgcagtgc ctgcgcctcc gggctcgaga cgcggcgcgg
      961 atgcgcgaac ggcacatcga aggacgcctg gatgcgcgcc ggccgcgggc tcagcaccag
     1021 gatccggtcc gccaggtgga tggcctcttc gacgtcgtgc gtgatcagca tgacggtgtg
     1081 cttctcgatc gacaggatgc gcagcagctc gttctgcatg cgctggctca tgagcgcatc
     1141 gagcgccgag aacggctcgt ccatgtagag gatttcaggt ttgaccacca gcgcgcgcgc
     1201 gatctcggcg cgcttcaaca tgccgccgga gagttcgtgc gggtagttgt tttcgaatcc
     1261 cttgagaccg acgagcgccg cgtattcatc cgcgagcgca tcgcgattcg gaagttcatc
     1321 cggcaagccg aacagcaggt tctgtttcac ggtcagccac ggaaaaaccg aaccctgctg
     1381 ggtgatgaga attcccttgg cgttaggctc tggacgcggc ttgccgtcga ccatgacggc
     1441 gcccgaatcc ggtgacagaa agccggccat gatgttcatc agcgtcgtct tgccgcaacc
     1501 ggatggtccg acgatggcca cgaactcgcc ttcgcgcacc tcgaagtcga ggccttcgat
     1561 gacctgcaga ttcaccttct cgttccggaa gcccttgcgg acgttctgca cgccaatctt
     1621 tattgaagaa ggtttagtgc gcataacgcc accgtacgac tttcatgctc tccagcaacc
     1681 tcatcaaacc atcgagcagc aggccgatga gcccgatgat gatcatgccg gcgatcacca
     1741 ggtcgtagcg atttccggcg ttgcgcgaat cgatgatcat gtagcccagg cccgagcgca
     1801 gcgcgatcat ctcggccgcc accacgacca gccaggcgac acccaggcca atccgcatgc
     1861 cgacgatgat ctgcggcagc gtggccggaa acaccacctg gagaaagagc ttcttgcgcg
     1921 acacgccgaa attgtccgcg gcgcgcaggt atcgcttctc gatggtgtgc acacccgagg
     1981 tggtctgcac gatcatcgga aaaaccgagg ctatgaa
//
LOCUS       DATDEU010000305         3966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1367063, whole genome shotgun sequence.
ACCESSION   DATDEU010000305 DATDEU010000000
VERSION     DATDEU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367063"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1886
                     /locus_tag="VIV63_07330"
     CDS             333..1886
                     /locus_tag="VIV63_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY6124447.1"
                     /translation="MTEPTPKKPQSSLRILVRNVLSNWTGLVLNMAVAFFMSPFLVHS
                     LGNVYYGLWVLIRSTTGYMGMLDAGVRVAVVKFVARAEASGDTTELNRTVSTAHFLFS
                     CAAAVAGVGTVIIAIAFPYIFKIDPETLGTARIVVLIAGVSLGVSLLSSVSGGILTGM
                     QRFDISNQIGIATLVIRTIVIVAMITMGYGIVALALTHLSMQIGNWIVINLVARRLRP
                     DIKANLSDANRQSAKQLYGYGSANVISGMGDLLLFRTGEILAAMFIGPAAVTYYAIAG
                     TLLEYLQKLVFTMAQTLHPFSSAKQAKGDERALQAVALIGTKLCFLIALPVTVGIVIF
                     GHDFIAAWMGASYALQAGPLLMAMAAGRLFWIAQSGTFQILLGAGRHKQAMIINVLTG
                     AFSFLGGLLCIQKYGLLGMTIGAAVPVVIIHGFITPIYTMRTLHIPLWRYAMESMLRP
                     MAATIPFAAALLAIYHWVHPQTLWGIALAGIAAAPLLVVSSYYLAFNREERLRYLHRF
                     LPKTRARPA"
     gene            1883..3037
                     /locus_tag="VIV63_07335"
     CDS             1883..3037
                     /locus_tag="VIV63_07335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6124448.1"
                     /translation="MKTRSLILMKWPTNVGYAIGALEKLFYEVALELADGDTSAVHFT
                     YTGVDAGAPSTLPGGFSQFYPLDINNLTPSALANLGEICRRERIDFAMPFDIQPVHPM
                     FPAMRSAGVRTIVPYWGAPISGMSPPWKRLIKRALLRLNSSRADGLIFESQAMADFAV
                     LGRGVPADDVDVVKLGVDIDRFKPDRGSRYVHESLGIPADRKVFVFTGHCTVRKGIST
                     LIDAAIEVLHKRGRQDICFLLCGNAGDESKPYEARYADLPIAPWIRFLGYRRDVLQIF
                     QSAYCGIIPSSGWDSFTLSSVEMAACGLPIIASRLQGLAEAVLHERTGLLFEPGNVTA
                     LADCIERMADNPERTRAFGDAGRQRAVSELTLGHQKEGMLRAIRRRLPAG"
     gene            complement(3038..>3966)
                     /locus_tag="VIV63_07340"
     CDS             complement(3038..>3966)
                     /locus_tag="VIV63_07340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6124449.1"
                     /translation="RSRRHLAPYDLLKVIRQLKPDIVHSHGEPPLAAAGPLGYLGLMP
                     FWAHSFHYGNYPYPESKRMFVERVLSRPAKALIAVADQQRGLIIKHHGVNPDRIRTLW
                     NGVKPNPFAGDAAHRARMRAEFGMADDDLVVGTVAVLSKQKGVTHLLQAARTVLASHP
                     KAKFLIAGGGPLEQALRDEAQALGLGPRVHFTGWRKDVQQILPAFDMFVMSSLWEAMP
                     LTLLEAMAARQALVVTDVGDNRLLVGNGEFALVVPPADSAALAGAITRLLDDRVLAAK
                     FAAAGAQAFAKQFTVGHMIAAHERFYESSVRV"
BASE COUNT          669 a         1359 c         1236 g          702 t
ORIGIN      
        1 cttcggtctg gctgtcatcg agaacgcgga tattttccat tcaaccatct cgccacaggg
       61 ggacaatcac acgccacata aggggaccgg gccatcttca tgggcgtgag ccaattcaca
      121 aattaccccg aagggacgcg catgtcgtcc tgctgcggcg ccgattcctg cagtctgaaa
      181 tctctccacc ggggaatcgc gaaaaagcct agcacgaagc cggccctgcg ggcgtgaacg
      241 aggcataaag cgaattcgaa cagggactac agcactgtga tgttcataat ccggcggcgg
      301 cgccaagccg ccgtccattc gcgcgcctga agatgaccga accgacccca aagaagccgc
      361 aatcgagcct gcgcatcctg gtgcgcaacg tgctgtccaa ctggaccggc ctggtgctca
      421 acatggcggt ggcgttcttc atgtcgccgt tcctggtgca cagcctgggc aacgtctatt
      481 acggcctctg ggtactcatc cgctccacca ccggctacat gggcatgctg gacgccggcg
      541 tgcgcgtggc ggtcgtcaag ttcgtggcgc gagccgaagc ctcgggcgac acgaccgaac
      601 tcaaccgcac ggtgagcact gcgcatttcc tgttctcgtg cgccgcggcc gtggccggcg
      661 tgggcacggt catcatcgcc atcgccttcc cttacatctt caagatcgat ccggagaccc
      721 tcggcaccgc gcgcatcgtg gtgctcatcg ccggtgtgtc gctgggcgta agcctgctgt
      781 cgtcggtctc gggcggcatc ctcaccggca tgcagcggtt cgacatctcg aaccagatcg
      841 gcatcgcgac gctggtgatc cgcaccatcg tcatcgtcgc catgatcacc atgggctacg
      901 gcatcgtcgc gctggcgctc acgcacctgt cgatgcagat cggcaactgg atagtcatca
      961 atctcgtcgc gcggcgcctg cgccccgaca tcaaggccaa cctgagcgac gcgaatcgcc
     1021 agtccgcgaa gcagctgtac ggctacggct cggcgaacgt gatcagcggc atgggcgacc
     1081 tcctgctgtt ccgcaccggc gaaattctcg cggccatgtt catcggcccc gccgcggtca
     1141 cctactacgc cattgccggc acgttgctcg aatacctgca gaagctggtg ttcacgatgg
     1201 cgcagacgct gcatccgttt tcgagcgcca agcaggccaa gggtgacgaa cgcgcgctgc
     1261 aggccgtcgc gctcatcggc accaagctgt gttttctcat cgcgttgccg gtgaccgtcg
     1321 ggatcgtgat cttcggccac gacttcatcg ccgcgtggat gggcgcgagt tacgcgctgc
     1381 aggccgggcc cttgctcatg gccatggccg cgggccgcct gttctggatc gcgcagtccg
     1441 gcacgttcca gatcctgctc ggcgcgggcc gccacaaaca ggccatgatc atcaacgtcc
     1501 tcaccggcgc gttcagcttt ctcggcggcc tcctctgcat ccagaagtac ggcctgctcg
     1561 gcatgacgat cggcgcggca gtgccggtgg tcatcatcca cgggttcatc acgcctatct
     1621 acacgatgcg cactctgcac atcccgctgt ggcgctacgc catggaatcg atgttgcggc
     1681 cgatggcggc caccattccc ttcgccgcgg cgctgctggc gatctaccac tgggtgcatc
     1741 cgcagacgct ctggggcatc gcgctcgcgg gcatcgccgc cgccccgctg ctggtggtgt
     1801 cgtcttacta cctggcgttc aaccgcgagg aacgcctgcg ctacctgcat cgcttcctcc
     1861 cgaagacgcg agcccggccg gcgtgaagac gcggagcctg atcctcatga agtggccgac
     1921 gaatgtcggc tacgccatcg gggctctcga gaagctcttc tacgaggtcg cgctggagct
     1981 ggccgacggc gacacctccg ccgtgcactt cacctacacc ggcgttgacg cgggcgcgcc
     2041 gtcgacgctg cccggcggct tctcgcagtt ctacccgctc gacatcaaca atctcactcc
     2101 ctcggcactc gcgaacctgg gtgagatctg ccggcgcgag cgcatcgact tcgccatgcc
     2161 gttcgacatc cagcccgtgc atcccatgtt cccggcgatg cgttcggccg gtgtgcgcac
     2221 catcgtgccg tactggggcg cgccgatttc cggcatgagc ccgccgtgga aacggctcat
     2281 caagcgtgcg ttgctgcgcc tgaactcttc gcgagccgac ggcctcatct tcgaatcaca
     2341 ggccatggcc gatttcgccg tgctgggccg cggcgttccc gcggacgacg tggacgtggt
     2401 gaagctgggc gtggacatcg accgcttcaa gcccgatcgc ggcagccgct acgtgcatga
     2461 gtcgctcggc atcccggccg atcgcaaggt cttcgtgttc accggccatt gcacggtgcg
     2521 caagggcatc agcacgctga tcgacgcggc catcgaagtc ctgcacaagc gcggacgcca
     2581 ggacatctgc ttcctgctat gcggcaacgc cggcgacgag agcaagccgt acgaggcgcg
     2641 ctacgcggat ttgccgatcg ccccgtggat ccgtttcctc ggctaccgcc gcgacgtcct
     2701 gcagatcttc cagagcgcgt actgcggcat cattccttcg tccggctggg attccttcac
     2761 gctgagctcc gtggagatgg cggcctgcgg attacccatc atcgcctccc gcctgcaggg
     2821 cctcgcggaa gcggtgttgc acgaacgtac gggcctgctg ttcgaacctg gcaacgtcac
     2881 ggcgctggcc gactgcatcg aacgcatggc cgacaatccg gaacgcacgc gcgccttcgg
     2941 cgatgcggga cggcaacgcg cggtcagcga gctcacgctc ggccatcaga aagaaggaat
     3001 gttgcgggcc attcgccggc ggctgccggc gggctaacta gacgcggacc gagctctcgt
     3061 agaagcgctc gtgcgcggcg atcatgtgac cgaccgtgaa ctgcttcgcg aaggcctgcg
     3121 cgcccgcggc agcaaacttc gcggccagca cccggtcatc gagcaggcgc gtgatcgcgc
     3181 cggccagcgc cgcggaatcc gccggcggca ccaccagcgc gaattctcca ttgcccacga
     3241 gcaggcgatt gtcgcccacg tcggtcacca ccagcgcctg gcgcgccgcc atggcctcga
     3301 gcaacgtgag cggcatggct tcccagagtg acgacatgac gaacatgtcg aacgccggca
     3361 ggatctgctg aacgtccttg cgccagcccg tgaagtgcac gcgaggcccg aggcccagcg
     3421 cctgcgcttc gtcgcgcagc gcctgctcga gcggaccacc gccggcgatc aggaatttcg
     3481 ctttcgggtg actggcgagc accgtgcgcg ccgcctgcag caggtgcgtg acacccttct
     3541 gcttcgacaa caccgcaacg gtcccgacaa cgagatcgtc gtcggccatg ccgaattccg
     3601 cgcgcatgcg cgcgcggtgc gcggcgtcgc cggcaaaagg attcggcttc acgccgttcc
     3661 acaacgtgcg gatgcgatcg ggattcacac catgatgctt gatgatgagc ccgcgctgct
     3721 ggtcggccac cgcgatgagc gccttggccg gacggctcag cacgcgttcg acgaacatgc
     3781 gcttcgattc cgggtaggga tagttgccgt agtggaaact gtgcgcccag aaaggcatga
     3841 ggccgaggta accgagcggg ccggccgccg ccaacggcgg ctcaccgtga ctgtgcacga
     3901 tgtccggctt taactgccgg atgaccttca gcaggtcgta gggcgcgagg tgccggcggc
     3961 tgcgct
//
LOCUS       DATDEU010000306         4204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1513201, whole genome shotgun sequence.
ACCESSION   DATDEU010000306 DATDEU010000000
VERSION     DATDEU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513201"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VIV63_07345"
     CDS             <1..272
                     /locus_tag="VIV63_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124450.1"
                     /translation="PFEVRIATRATDSGDVEVSVSDLGPGLAGEAEQKIFEPFFTTKA
                     SGTGLGLAISRSIIKAHDARLGYRANQPRGACFYFVLPAHQEAPS"
     gene            269..886
                     /locus_tag="VIV63_07350"
     CDS             269..886
                     /locus_tag="VIV63_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124451.1"
                     /translation="MKDLPQTVFVVDDDDAVRDSLRMLLKSAGVHAEACASAQEFLNA
                     YDASQPGCLVLDVRMPGMSGLEMQHELNLRGLTMPVIFITGHGDIPMAVEAMQHGAFD
                     FLQKPFRDQELLDRVQRALVRDTENRARLRHTDRIRERLASLSPREREVLDLVTQGKA
                     NKMVAGDLGVSQRTVEIHRAHVMQKMEAGSLAELVRMMMAVAPPP"
     gene            956..1366
                     /locus_tag="VIV63_07355"
     CDS             956..1366
                     /locus_tag="VIV63_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124452.1"
                     /translation="MAGTNENGSGLNGDRGTQRVRTIIVAGQSSQRTREVLRALSGFT
                     NVIWPTSLESVLAQTRSKDAGCLVIADDLREEPIDEFLQEARELQRSLPIFVITDPDD
                     VAEAVSAMRHGATAVIEIPPSYAALCEEVSRAMQ"
     gene            1501..1956
                     /locus_tag="VIV63_07360"
     CDS             1501..1956
                     /locus_tag="VIV63_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124453.1"
                     /translation="MSAQLANVVALKLHEPSPHGLILHVDDDEAIRLSTAMLLRSAGF
                     ETREAAYGEQALAQAESLRGRLDVLIVDYDLGRSMTGTEVAEALARLLGHAVPTVILT
                     GDPANAEVPWLADAPVWVSRKPLAPNTLLAALPPLVAFRRAVAAAPHIR"
     gene            complement(1967..2776)
                     /locus_tag="VIV63_07365"
     CDS             complement(1967..2776)
                     /locus_tag="VIV63_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY6124454.1"
                     /translation="MNRTKLLSLIVAAALFSSGCETVKTTSAGAVGVDRKQQMLVPSA
                     TIEQGAAEAYEAELKAARDKGVLNTDKAQLARVTTIARRIVGATPAFRGDAAAWNWQF
                     NVQKTKDLNAYCMPGGRIMVYSGLIENLDLSDAELATVIAHEVAHALREHTRERVSRA
                     YAQQLVLSGAAAVTGVGEGAANIANMVAEVTFQLPFSRDQESEADQIGLELMARAGYD
                     PRAAITLWNKMSAASSGGQPKFLSTHPAAKDRIKDIEKNLPRVLPLYAQRR"
     gene            3180..>4204
                     /locus_tag="VIV63_07370"
     CDS             3180..>4204
                     /locus_tag="VIV63_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6124455.1"
                     /translation="MSESIKIAVKNAIRFRARAAFAAAALYGFASAAVAQDANPADAP
                     EGAEQIEEVVVTGFRASLNKALEAKQEQVGSIDMIVAEDIADFPDLNLAESLQRVPGV
                     VIARDAGEGRQISVRGLGPQFTRVRINGIEAMSANGSTDASGGTNRGRNFDFNTFASE
                     LFSNITVRKTAAAETEEGSLGATVDLQTGRPFDFDGFTMAGSLQAGYNDLTEESDPRA
                     AFLISNTFADGKFGALMSIAYTDRKLADDGSSTVRWQNSGTCPGTGCFGPLELGYTSA
                     PTLAQLNAAFRPRIPRYDKYEHDQQRLGVTGSLQFAPTDATSFNLDVLFAQFDAERDE
                     IFLETPV"
BASE COUNT          798 a         1365 c         1262 g          779 t
ORIGIN      
        1 cgccgttcga agtgcgcatc gcgacccgcg ccaccgactc gggcgacgtc gaagtctcgg
       61 tgagcgacct gggaccgggc ctggctggcg aggctgaaca gaagatcttc gaaccgtttt
      121 tcaccaccaa ggcatcgggc acgggattag ggctggcgat cagccgctcc atcatcaagg
      181 ctcacgacgc ccgcctgggc tatcgcgcca atcagccgcg aggcgcatgc ttctacttcg
      241 tccttccggc ccaccaggag gctccatcat gaaagacctg ccgcaaaccg tgtttgtggt
      301 cgatgacgac gacgcggtac gcgactcgct gcggatgctg ctcaagtccg ccggggttca
      361 cgcggaggcc tgtgcatccg cgcaggaatt tcttaacgcc tacgacgcct ctcagcccgg
      421 ctgcctggtg ctcgacgttc gcatgcccgg aatgagcgga ctcgagatgc agcacgagct
      481 gaatctgcgt ggactaacca tgccggtcat attcatcacg ggccacggcg acattccgat
      541 ggcggtcgaa gccatgcaac acggcgcctt cgatttcctt cagaagccat tccgggatca
      601 ggaattactc gaccgcgtgc aacgcgccct ggtgcgtgac acggaaaacc gggcgcgcct
      661 ccggcacacc gacaggattc gcgagcggct cgcatccctt agtccgcgcg agcgcgaagt
      721 gctcgatctc gtcacgcagg gcaaggcaaa caagatggtg gcgggcgacc tgggtgttag
      781 tcagcgcacc gtggagattc accgcgcgca cgtgatgcag aaaatggaag ccgggtcgct
      841 ggctgagctg gtccgcatga tgatggctgt tgcgcctcca ccatagagtc gcgcgcacga
      901 cacgaaaaag tacgtattta tcagaccaca gcccgatgca atgctccggc ccgaaatggc
      961 gggcaccaat gaaaacggct cggggttgaa cggcgatcgc ggaacccaac gcgtgcgcac
     1021 catcatcgtc gccggtcaat cttcccagcg cacccgtgaa gtcctgcgcg cgctgagtgg
     1081 cttcaccaac gtcatctggc caacatccct ggaatccgtg ctggcgcaga cgcgctcgaa
     1141 ggacgcgggc tgcctcgtca tcgcagacga cctgcgcgag gaacccatcg acgagttcct
     1201 gcaggaagcg cgcgaactgc agcgaagcct gcccatcttc gtgataaccg atccagacga
     1261 tgtcgcagaa gccgtttccg ccatgcggca cggcgcaact gcggtcatcg aaattccgcc
     1321 gtcgtacgcg gctctttgcg aagaagtcag tcgcgcaatg cagtaagcgc agtccgacag
     1381 cgacacaatc gtgtcatccg gcaactgcat catcgtcttg tccgtattga cgccttgatc
     1441 gcgcgagaga aaacttgcgc ggcagcatct cctcactacc gtagtcacgc agggcgaacc
     1501 atgtccgcgc aactcgccaa tgttgttgca ctcaaactcc acgaaccatc tccgcacggg
     1561 ctcatcctgc acgtcgacga cgacgaagcg atacggctct cgacggcgat gttgttgcga
     1621 tcggctggtt tcgaaacgcg ggaagcggcc tatggcgagc aggcgctcgc gcaggcggag
     1681 tcgcttcgcg gccggctcga cgtgctgatc gtcgactacg acctcgggcg ctcgatgacc
     1741 ggcaccgagg tcgccgaggc gctcgcaagg ttgttagggc acgcggtgcc cacggtgatc
     1801 ctcaccggcg atcccgccaa cgccgaagtt ccgtggctcg ccgatgcgcc agtgtgggtg
     1861 tctcgcaaac cgcttgcgcc aaacacgcta ttggcggcgc tacccccgct ggtcgcattc
     1921 cggcgcgccg tagcggcagc gcctcacatt cgctgacgcg gatttttcag cggcgctgcg
     1981 cgtacagcgg cagtacgcgc ggcagattct tctcgatgtc cttgatccga tccttcgcgg
     2041 cgggatgcgt gctcaggaat ttcggctggc cgccgctcga ggcggctgac atcttgttcc
     2101 acaacgtgat cgcggcccgc gggtcgtaac cggcgcgcgc catgagctcg aggccgatct
     2161 ggtccgcttc ggattcctgg tcgcggctga agggcaactg gaacgtcact tcggcaacca
     2221 tgttggcgat gttcgccgcg ccctcgccga ctcccgtgac cgcggcggcg ccggacagca
     2281 ccagttgctg cgcatacgcg cgcgatacgc gctcgcgcgt gtgttcacgc agcgcatgag
     2341 ccacctcgtg cgcaatcacg gtagcgagct ccgcgtcgga gaggtccagg ttttcgatca
     2401 ggcccgaata caccatgatg cggccgcccg gcatgcagta ggcgttcaga tccttcgtct
     2461 tctggacatt gaactgccag ttccaggcgg cagcgtcgcc gcgaaacgcc ggtgtcgcac
     2521 cgacgatgcg ccgcgcaatc gtcgtgacac gcgcgagctg tgccttgtcc gtgttgagta
     2581 cgcctttgtc gcgcgcggcc ttgagctccg cctcgtaggc ttcggcagca ccctgttcga
     2641 tggtggcgct cggcaccagc atctgctgct tgcgatcgac gccgacggcg cccgcgctgg
     2701 tggttttgac ggtttcgcag ccgctcgaga agagcgcggc ggcgacaatg aggctcagga
     2761 gtttcgttcg gttcatcgcc tggatactcc acgttgctgt ttgcgctaac aatcaacctt
     2821 cgcgacgcaa gaagttagga catgcgcgat tgtcgtgcag gttttctcct actaaccctc
     2881 gtacggtgca aggggaaccg actaggagtt ggtcacgaat gttaggcctg tgaactccgt
     2941 cgcaaaacgt cgcacaaatc acacagacgt gaaacacgag tgcgtgcaca attcggcccg
     3001 ttggactcac atatacgagt gaaaacatag gcttcagtcg tattgccctg gtgatgttgt
     3061 caccggtgtc atttttgtgg gataaagcgc gtcggcagct ccttagtcgc ggctgccgat
     3121 gcacaccgtt cacttgtttg cgtacgcctt ggatgaggcg catgtctgga gggaccaaga
     3181 tgtccgaatc gatcaaaatc gccgtaaaaa atgccattcg tttccgcgcg cgagccgctt
     3241 tcgctgcagc cgccctgtac ggcttcgcaa gcgccgccgt cgcgcaggac gccaatcccg
     3301 ctgacgcgcc cgaaggcgcg gagcagatcg aggaggtcgt agtaaccggt ttccgcgcga
     3361 gcctcaacaa agcactcgaa gcgaagcagg aacaagtcgg ttcgatcgac atgatcgtcg
     3421 ccgaggacat cgccgatttt ccggatctca accttgccga atcactgcaa cgcgttcccg
     3481 gtgtcgtgat cgcgcgtgac gccggcgaag gccgccagat ctccgtgcgc ggactcggcc
     3541 cgcagttcac gcgcgtgcgc atcaacggca tcgaagccat gagcgccaac ggcagcaccg
     3601 acgcgtcagg cggcacgaac cgcggacgca atttcgactt caatactttc gcttccgaac
     3661 tcttcagcaa catcacggtg cgcaagactg ccgccgctga aaccgaagaa ggatcgctcg
     3721 gcgccacggt cgatctgcag accggccgtc cgttcgattt cgacggcttc accatggccg
     3781 gctcgctgca ggccggctac aacgacctca ccgaagaaag cgacccgcgt gccgcgttcc
     3841 tcatcagcaa cacgtttgcc gatggcaaat ttggcgcgct gatgtcgatc gcctacaccg
     3901 accgcaaact ggccgacgat ggatccagca cggttcgctg gcagaactcg ggcacttgcc
     3961 cgggcaccgg ctgcttcggt cctctcgagc ttggctacac gagcgcgccg acgttggccc
     4021 agctcaacgc cgcgttccgc ccgcgcatcc cgcgctacga caaatacgag catgaccagc
     4081 agcgcctcgg cgtcaccggt tcgttgcagt tcgcgcccac ggacgcgacc tcgttcaatc
     4141 tcgatgtgct gttcgcccag ttcgatgccg agcgcgacga aatcttcctc gaaactccgg
     4201 tgtt
//
LOCUS       DATDEU010000307         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1367563, whole genome shotgun sequence.
ACCESSION   DATDEU010000307 DATDEU010000000
VERSION     DATDEU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367563"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /locus_tag="VIV63_07375"
     CDS             complement(<1..2018)
                     /locus_tag="VIV63_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124456.1"
                     /translation="MRTEGLLKQSQELAGELQTQQVELQQTNEEMATKARLLAEQNAE
                     VERKNQEIEQARKALEEKAAELALTSRYKTEFLANMSHELRTPLNSILILGQQLSENP
                     EANLSARQVEFAKTIHAAGTDLLNLISDILDLSKIESGTVTVESEDLAFSQLRETIER
                     NFRHEAESRHLTFTTDFDPRLGRNMVTDAKRLMQIVKNLLSNAMKFTAQGSVRLHVGP
                     AMLGWTADHPVLSQAKHVVEFAVTDTGIGISPEKQRIIFEAFQQADAGTARKYGGTGL
                     GLAISRELAHLLGGEIRLKSSPGSGSTFTLYLPLTYQGAAYGRGTTPTFIKQPSAALP
                     APALPAPHIERVEDDRDSIEEGESTVLIVEDEPRYARMLLEAAHGKGFKVLVAQNGAD
                     ALSLARKYQPDAVTLDVFLPDMLGWTVLNQLKHDPATRHIPVQVLTVEEERQYGLERG
                     AFSFMTKPTTVEGLQSVFTRIQEFIEPRVRELLVIEDDSAERLSVAALIEASDIRIST
                     AESGARALELLRERKFDCVILDLKLPDISGFELLTEIQRDERLREIPIVVFTGRELTH
                     EEEGELRQRAKSIVLKGVQSPERLLDETALFLHRVVGDLPPAKREMLRNLHQSDASLL
                     GKKVMVVDDDVRNIFAISSILERHGMDVVTANNGQEAIDRVEQEPDIA"
BASE COUNT          296 a          666 c          671 g          406 t
ORIGIN      
        1 atggcgatgt ccggctcctg ctccacccgg tcgatcgcct cctggccgtt gttggcggtc
       61 accacgtcca tgccgtggcg ctccaggata ctgctgatgg cgaagatatt gcggacgtcg
      121 tcgtcgacca ccatcacctt cttgcccagc agcgaggcat cggactggtg caggttgcgc
      181 aacatctcgc gcttggccgg cggcaggtca ccgacgacac gatgcaggaa cagcgccgtc
      241 tcgtcgagca ggcgttcggg gctctgcacg cctttcagca cgatgctctt cgcgcgctgg
      301 cgcagttcgc cttcttcctc atgcgtgagc tcgcgcccgg tgaacaccac gatcgggatc
      361 tcgcgcagcc gttcgtcgcg ctgtatctcg gtcagcagtt cgaacccgga aatgtccggc
      421 agcttgagat cgaggatcac gcagtcgaac ttccgttcgc gcagcagctc tagcgcgcgg
      481 gcgccgctct cggccgtgga aatgcggatg tcggaggcct cgatgagcgc cgcgacgctc
      541 agccgctcgg ccgagtcgtc ctcgatgacg agcagctcgc gcacacgcgg ttcgatgaat
      601 tcctggatcc tcgtgaacac ggactgcagc ccttccacgg tggtcggctt ggtcatgaac
      661 gagaacgcgc cgcgctcgag cccatactgg cgctcttctt cgaccgtgag cacctgcacg
      721 gggatgtggc gcgtggccgg gtcatgcttg agctggttga gcaccgtcca ccccagcatg
      781 tcgggcagga acacgtcgag cgtgaccgcg tcgggctggt acttgcgcgc gagcgagagc
      841 gcgtccgcgc cattctgcgc gaccagcacc ttgaagccct tgccgtgcgc ggcttccagc
      901 aacatgcggg cgtagcgcgg ctcgtcttcg acgatcagca ccgtgctctc gccttcctcg
      961 atggaatcgc ggtcgtcctc gacgcgctcg atgtgcggcg ccggcagcgc cggagccggc
     1021 agcgcggcgg acggctgctt gatgaacgtc ggcgtcgtgc cgcgaccgta ggccgcgccc
     1081 tggtaggtca gcggtagata gagcgtgaac gtgctgcccg atcccggcga gctcttgagg
     1141 cggatttcgc cgcccagcag gtgcgcgagt tctcgcgaga tggcgagccc caggccggta
     1201 ccgccgtact tgcgggccgt gcccgcgtcc gcctgctgga acgcctcgaa gatgatcctc
     1261 tgcttctccg gggagatgcc gatgcccgtg tcggtgaccg cgaattcgac cacgtgcttg
     1321 gcctgcgaca gcaccggatg atcggcggtc cagcccagca tcgcgggacc cacgtgcagg
     1381 cgcacgcttc cctgcgccgt gaacttcatg gcgttcgaca gcaggttctt cacgatctgc
     1441 atgagacgct tcgcgtccgt gaccatgttg cggccgaggc gtggatcgaa atcggtcgtg
     1501 aacgtgagat ggcgcgattc cgcctcgtgg cggaagttgc gctcgatcgt ctcgcgaagc
     1561 tgcgagaacg cgagatcttc actctccacc gtcaccgtgc ccgactcgat cttcgacagg
     1621 tcgaggatgt cgctgatgag gttcaggagg tcggtgccgg cggcgtgaat ggtcttggcg
     1681 aattccacct ggcgtgccga cagattggct tccgggttct cggagagctg ctgccccagg
     1741 atcaggatgc tgttgagcgg cgtgcgcagc tcgtgcgaca tgttggccag gaactcggtc
     1801 ttgtagcgcg acgtgagcgc gagctcggcc gccttttcct cgagggcctt gcgggcctgc
     1861 tcgatttcct ggttcttgcg ctccacttcc gcgttctgct cggccagcag ccgggccttg
     1921 gtggccattt cctcgttcgt ctgctgcagc tcgacctgct gcgtctgcag ctcgccggcg
     1981 agctcctgcg actgcttgag cagaccctcg gtgcgcatcg tcgcttcgat ggtgttcag
//
LOCUS       DATDEU010000308         4477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1805432, whole genome shotgun sequence.
ACCESSION   DATDEU010000308 DATDEU010000000
VERSION     DATDEU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805432"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VIV63_07380"
     CDS             <1..321
                     /locus_tag="VIV63_07380"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124457.1"
                     /translation="DLGAIRGCGFEDVGDEPFDIIINATAAGLQGAVPNVDAAIIASH
                     SVCYDMSYSKASTPFVTWAIEQGCARAHKGWGMLVEQAAEAFTLWRGVRPDTEPVLAV
                     LGNK"
     gene            318..2336
                     /locus_tag="VIV63_07385"
     CDS             318..2336
                     /locus_tag="VIV63_07385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY6124458.1"
                     /translation="MTDANISGTTLPAFDRINAEGVEPAIRELLQRNRGEVATLESQP
                     QPTFANTVLPLEQLSHRLSRTWSPISHLNGVLNSDALRASYNACLPLLSNYWTDLAQS
                     EPLFRAYTAIAVNESGSLDPAQRRVIERALEDFRLAGVGLPPERKARYKTVVQELATL
                     AAKFEENVLDAMNAWTRHVTDETELAGINPVIVEQARVRAAEKGVPGWLFALEQPAYV
                     AVMTDAHSEGLRRDFYEAWSTRASERGPTAGKFDNTPVMNRILALRHEAAQLLDFPNY
                     AAYALATRMAPSNQAVFDFLRKLVGVARPAAKREFTELEAFAGRPLNAWDVSYYSQKL
                     QQKLFSISQEELRPYFPLPRVLEGLFGVAEQLFGVKIAERKGVPVYHPDARFFEILDA
                     KGEVRAGFYLDACARAKKRAGAWMDDCVGRKDFGAEHTLPVAYLVCNFLPAAPGSPAL
                     LTHDDVVTMFHEFGHGLHHMLTRVGYPSIAGINGVSWDAVELPSQFMENFAWRPEVLA
                     SMARHYETGAPLPAEKQAQLLGSRTFHAGLATMRQLEFALFDFRLHAEYDPAQGARVH
                     EILAEVRDEVAAFAVPEWNRFPHNFGHIFAGGYAAGYYSYKWAEVLSADAFAAFEEQG
                     VFDKAVAQRFLDSILSQGGAKKALDAFVDFRGRPPDVNALLRRQGLVQ"
     gene            2333..3175
                     /gene="xth"
                     /locus_tag="VIV63_07390"
     CDS             2333..3175
                     /gene="xth"
                     /locus_tag="VIV63_07390"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY6124459.1"
                     /translation="MNAATDNGGSAAKGEFSIATWNVNSLRVRGEQLLEWLRANSPDV
                     VALQETKMQDPDFPHAEFTAAGYHSVFSGQKTYNGVALLSRHPMTDIVRDIPDFDDPP
                     RRVLAASIVGPWGDFRVINLYVPNGQAPGSEKFAYKLRWLEALTRWLKLEASMHPGLV
                     VLGDFNVAPEDRDVHDPAAWVGNVHVSPEERAALARITDLGLVDAFRHFEQPEKSFSW
                     WDYRQGAFRRNNGLRIDLILAHRQLVPRLAGCRIDVEPRRVERASDHTPVIVSFGMGT
                     LPIS"
     gene            3368..4366
                     /locus_tag="VIV63_07395"
     CDS             3368..4366
                     /locus_tag="VIV63_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124460.1"
                     /translation="MIAESRRSDYDVTDTVLVSSAPDVRAAIEELFAKVWPDQKFPAF
                     AHVFGDFERMFSGRMPGYFGVDTVYHDQQHTLDITLATARLIVGHEMQVPEEQKFGAE
                     RAMLGIVVALFHDVGYLRTTRDTDVPNGAEFTTVHVSRGAAFLTDYLPKINLGQWTKV
                     AAEIIHYTGYERPFKNIVAPDPRDHKLGHLVGTADLMAQMADRCYLEKCRDRLYAEFV
                     LAGVALPIGENGHVKVRYASGLDLLRQTPKFVFDMRAKRLDGEFNRAYRYLEVLYKGR
                     NPYMESIDRNLEYLQRILRSENWRLLRRQPPVFTGVEDSMATTRTLMLGAIKKVWG"
BASE COUNT          806 a         1523 c         1382 g          766 t
ORIGIN      
        1 gatctcggcg cgatccgtgg ctgcggattc gaggacgttg gcgacgagcc cttcgacatc
       61 atcatcaacg ccaccgccgc cgggttgcag ggcgcagtgc cgaacgtcga tgcggcgatc
      121 atcgcgagcc actccgtctg ctacgacatg agttacagca aggcatccac tcctttcgtg
      181 acctgggcca ttgaacaggg ctgcgccaga gcccataaag gctggggcat gctcgtcgaa
      241 caggcggccg aagcgttcac cttgtggcgc ggcgtgcgcc ccgacaccga acccgtcctg
      301 gccgtccttg gaaacaaatg accgacgcaa acatcagcgg gaccacgctt cccgcgttcg
      361 accgcatcaa tgctgaaggc gtggaacccg ccatccgcga gctcctgcag cgcaaccgcg
      421 gcgaggtcgc gacgctcgag tcgcagccac agccgacgtt cgccaacacc gtgttgccgc
      481 tcgagcagct ctcgcatcgc ctgtcgcgca cctggtcgcc catcagccac ctgaatggcg
      541 tgctcaattc cgacgcgctg cgcgcgagct acaacgcctg cctgccgctg ctgtctaact
      601 actggaccga cctcgcgcag agcgagccgc tgttccgcgc ctacacggcc atcgccgtca
      661 acgaatccgg ctcgctcgat ccggcgcagc gccgcgtcat cgaacgcgca ctcgaggact
      721 tccgtctcgc cggcgtaggc ctgccgcccg agcgcaaggc gcgctacaag accgtggtcc
      781 aggaactggc gacgctcgcc gccaagttcg aagagaacgt gctcgacgcg atgaatgcgt
      841 ggacacgtca cgtcaccgac gaaaccgaac tcgccggcat caaccccgtg atcgtcgagc
      901 aggcgcgcgt gcgcgccgcc gaaaagggcg tgccgggatg gctgttcgcg ctcgaacagc
      961 ccgcctacgt cgccgtgatg acggacgccc actccgaagg attgcgccgc gatttctacg
     1021 aggcgtggtc gacacgcgcg tcggagcgcg gaccgaccgc cgggaagttc gacaacaccc
     1081 cggtcatgaa tcgaatcctc gcgttgcgcc acgaagccgc gcagctgctc gatttcccca
     1141 actacgcggc gtatgcgctg gcgacgcgca tggcgccgtc caaccaggcg gtgttcgatt
     1201 tccttcgcaa gctggtgggt gtcgcgcgcc cggccgcgaa gcgcgaattc acggagctcg
     1261 aggcgttcgc cggccgcccg ttgaacgcct gggacgtcag ttactactcg cagaagctgc
     1321 aacagaagct gttttcgatc tcgcaggaag aattgcggcc ctacttcccg ttgccgcgcg
     1381 tgctcgaagg cctcttcggc gtggccgagc agttgtttgg cgtgaaaatc gcggagcgca
     1441 aaggcgtacc ggtttatcac cccgacgcac gcttcttcga aatcctcgat gcgaaaggcg
     1501 aggtccgcgc cggtttctac ctggatgcct gcgcgcgcgc gaagaaacgt gccggcgcgt
     1561 ggatggacga ctgcgtgggc cgcaaggact tcggcgccga acacacgctg ccggtggcct
     1621 acctcgtgtg caatttcctg cctgccgcac cgggctcgcc ggcgctgctc acgcacgacg
     1681 acgtcgtgac catgttccac gagttcggcc atgggcttca ccacatgctc acgcgcgtgg
     1741 gatatcccag catcgccggc atcaacggcg tgtcctggga cgcggtcgag ctcccgagcc
     1801 agttcatgga aaattttgcg tggcgccccg aggtgctcgc gtccatggct cgccattacg
     1861 aaacaggcgc accgctaccc gcggagaaac aggcgcagtt gttaggctcg cgcactttcc
     1921 acgccggcct cgcgaccatg cgtcagctcg aattcgcgct gttcgacttc cgcctgcatg
     1981 ccgaatacga cccggcccag ggtgcgcgcg tccacgaaat cctcgccgag gttcgtgacg
     2041 aagtcgccgc gttcgccgtg ccggagtgga atcgcttccc gcataacttc gggcacatct
     2101 tcgccggcgg atacgccgcg ggttactaca gctacaaatg ggctgaagtg ctcagcgccg
     2161 acgcattcgc cgcgttcgaa gagcagggcg tgttcgacaa ggccgtcgcg cagcgcttcc
     2221 tcgactccat cctgtcgcag ggtggcgcga agaaggcgct cgatgcgttc gtcgactttc
     2281 ggggccgccc gccggatgtg aacgcgctgt tgcgccggca gggcctcgtc cagtgaacgc
     2341 cgccaccgac aacggtggct cagcggcgaa gggtgagttc tcgatcgcca cctggaacgt
     2401 gaacagcctg cgcgtgcgcg gcgaacaact gctcgaatgg ctgcgcgcga attccccgga
     2461 cgtggtcgcg ctgcaggaaa cgaagatgca ggacccggac ttcccgcacg cggagttcac
     2521 cgccgcggga tatcacagcg tcttcagcgg gcagaagacc tacaacggcg tcgccctgct
     2581 ctcgcgccac ccgatgacgg acatcgtgcg cgacatcccg gacttcgacg atccgccgag
     2641 gcgtgtactg gccgccagca tcgtgggtcc gtggggcgac ttccgcgtca tcaatctcta
     2701 cgtgcccaat ggccaggcgc cgggttctga aaaatttgca tacaagctcc ggtggctcga
     2761 ggcgctgaca cgctggctga aactcgaggc ttcgatgcat cccgggctgg tggtgctcgg
     2821 cgatttcaat gtcgcgcccg aggaccgcga tgtgcacgac cctgccgcgt gggtgggcaa
     2881 cgtacacgtg agccccgagg agcgcgccgc gctcgcgcgc atcacggacc tgggccttgt
     2941 ggatgccttc cggcatttcg agcagccgga gaaatcgttc agctggtggg actatcgaca
     3001 gggcgcattc cggcgcaaca acggtctgcg catcgatctc atcctggcgc accgtcaact
     3061 ggtgccccgg cttgccggtt gccgcatcga cgtggagccc cggcgcgtgg agcgggcgtc
     3121 ggatcacacc cccgtcatcg tgagcttcgg aatggggaca ctccccattt cttagcaaat
     3181 tgggccgcag ctgaagcggg tccgtcggag cgctttacag taataagttc cccgcggcga
     3241 ttgtggcttc gccccaccgt gaagcgtgtc acgcctgctc cagactcaga ttccgcccgc
     3301 tggtctttca gcccccccct ccctagactc gcttcatctt gttagtgctc gaaggcatgg
     3361 cacggccgtg atcgcagaat cccgccgcag cgactacgac gtaaccgaca cggtgctcgt
     3421 gagctcggct cccgacgtcc gtgcggctat cgaagagctg ttcgccaaag tctggccgga
     3481 ccagaaattt cccgcgttcg cccacgtttt cggtgacttc gaacgcatgt tctccgggcg
     3541 catgcccggg tacttcggcg tcgacaccgt ctatcacgac cagcagcaca cactcgacat
     3601 cacgctcgcc acggcgcggc tcatcgtcgg ccacgagatg caggtcccgg aggagcagaa
     3661 gttcggcgcc gagcgcgcca tgctcggcat cgtggtcgcg ctgttccacg acgtcgggta
     3721 cctgcgcacc acgcgcgaca ccgacgtgcc caatggcgcc gaattcacca ccgtgcacgt
     3781 ctcgcgcggc gcggcattcc tgacggacta tctacccaag atcaatctcg ggcagtggac
     3841 caaggtggcc gccgagatca tccactacac cggctacgag cggccgttca agaacatcgt
     3901 cgcgcccgac ccgcgcgatc acaagctcgg gcacctggtc ggcaccgccg atctcatggc
     3961 gcagatggcc gatcgctgct atctcgagaa gtgccgcgac cggctctatg cggagttcgt
     4021 actcgccgga gttgcgctgc cgatcgggga aaacggccac gtgaaggtgc gctacgcgtc
     4081 gggtctcgac ctgctgcgcc agacgccgaa gttcgtgttc gacatgcgcg ccaagcggct
     4141 cgacggtgaa ttcaaccgcg cgtaccgcta cctggaagtc ctgtacaagg gccgcaaccc
     4201 gtacatggaa tccatcgatc gcaacctcga gtacctgcag cgaatcctgc gcagcgaaaa
     4261 ctggcgcctg ctgcggcgtc agccgccggt gttcacgggc gtcgaagact ccatggccac
     4321 cacgcgcacg ttgatgctcg gggccatcaa gaaagtctgg ggttgagccg aaaggctcac
     4381 caaaaaaaga cagtctcccc tcaggcgagc cgtggcgaca gcaacgccag ctcaggcgcc
     4441 gcgtccacgt tgaccacata ggtcgcgtag ttgcgat
//
LOCUS       DATDEU010000309         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1221877, whole genome shotgun sequence.
ACCESSION   DATDEU010000309 DATDEU010000000
VERSION     DATDEU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221877"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VIV63_07400"
     CDS             <1..817
                     /locus_tag="VIV63_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124461.1"
                     /translation="MFFPRVVAFDRLRGRFELFAPCGVAAGIIARCDESTPVWAAAEG
                     DAAVLRHGMRPAWNVNNADRVRLANAGINLLQAVRLAPKFATSPRTLASGSAAVSDWR
                     YLSARRLALFIVTSIERGTRWMLFERNSQPTWTMARAQVEAFLDSLYGEGAFAGRSGE
                     QSYFVVCDERINTEESIRAGKMNLAFGFAATKPGEYHAFIITHQPSGSRAKVISVNRF
                     ANYSPSVDLEIESSILRGLVPLAPVVPPLGPVAEMPEPTTTVMEAPLAPYREA"
     gene            complement(836..1243)
                     /locus_tag="VIV63_07405"
     CDS             complement(836..1243)
                     /locus_tag="VIV63_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124462.1"
                     /translation="MTAESKTSVPVRNSTHILIVDDNREAGKSLAQMLTDSGCDEIRT
                     VRSAARALAIAATFQPAIAFLGIPTPDGLEGNLAALLQKNAREKNIRLIGLTDLAEPP
                     GREEARALGVERFISKPVTQLELDKALRKSPGA"
     gene            complement(1333..>2178)
                     /locus_tag="VIV63_07410"
     CDS             complement(1333..>2178)
                     /locus_tag="VIV63_07410"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY6124463.1"
                     /translation="LLLALPTAALYVRLFILQHDCSHGSFFRSSRANRWLGASLGLLT
                     LFPFAYWKKTHGVHHATSGNLDQRELGDIRTMTVSEYRASSPWMRFCYRFYRSMPVML
                     GIGPFYQFVAKHRFPLGMPWSWKKEWRSVALNNLSLLALGVVLSVWLGWGVVLAAHLP
                     VVAIAGALGVWLFYVQHSFEESYWVRRSDWDPKAAAVDGSSFYDLPAVLRWITGNIGY
                     HHIHHLAPRIPNYHLRAAHEAIPSLKPTVRMGLRESLACARMKLWDESLGRMVGFPRS
                     AARRT"
BASE COUNT          365 a          709 c          740 g          364 t
ORIGIN      
        1 catgtttttc ccgcgcgtgg tggcgttcga ccggctgcgt ggccgcttcg agctgttcgc
       61 gccctgcgga gtggcggcgg gcatcatcgc gcgctgcgac gaaagcacgc cggtgtgggc
      121 agcggcggaa ggcgatgccg ccgtcctgcg ccacggcatg cggccggcct ggaacgtgaa
      181 caacgccgac cgcgtgcgcc tcgcgaatgc cggcatcaac ctgctgcagg cggtgcgcct
      241 cgcgccgaaa ttcgcgacca gcccgcgcac gctcgcgagc ggctccgccg ccgtcagcga
      301 ctggcgttac ctgtcggcgc gccgcctcgc attgttcatc gtgacttcca tcgagcgcgg
      361 cacgcgctgg atgctgttcg agcgcaattc gcagcccacc tggaccatgg cgcgcgccca
      421 ggtcgaagcc ttcctcgatt cgctgtacgg cgagggcgcg ttcgccggcc gtagcggcga
      481 gcagagctat ttcgtcgtgt gcgacgagcg catcaatacc gaagagtcca tccgcgcggg
      541 caagatgaac ctcgccttcg gattcgcggc caccaagccg ggcgaatacc acgcgttcat
      601 catcacgcac cagccgagcg gctcgcgcgc gaaggtgatc agcgtgaatc gcttcgcgaa
      661 ctattcgccg agcgtggatc tggagatcga atcgagcatt ctgcgcgggc tggtgccgct
      721 ggcgccggtc gtcccgccgc tcgggccggt cgctgaaatg cccgagccga cgacgacggt
      781 catggaagcg ccgctcgcgc cttaccgcga agcgtagatt tcgcgcgcgc ggcggtcagg
      841 cgccaggcga tttcctcaaa gccttgtcga gttccagctg cgtcaccggc ttgctgatga
      901 atctctcgac gcccaaggcc cgtgcttctt cccggcccgg cggttccgcc aggtcggtga
      961 ggccgatgag ccggatgttc ttctcgcggg cattcttctg cagcagcgcc gcgaggtttc
     1021 cttcgagccc gtcgggcgtc gggataccga ggaacgcgat cgccggctga aacgtggcgg
     1081 caatcgccag cgcgcgtgcg gcgctgcgta cggtgcggat ctcatcgcag ccgctgtcgg
     1141 tgagcatctg cgccagcgat tttcccgcct cgcgattgtc atcgacgatg aggatgtgag
     1201 tgctgttgcg gacgggaacg gaagtcttgg actcggcggt catcgggcgt gtactccagg
     1261 gcgccggatg cgcgggccag caggatggca ggtcttgagt ttcctgcccg ggtattctga
     1321 attcggggcg attcaggtgc ggcgggccgc cgaacgggga aatccgacca tccgccccag
     1381 gctctcatcc cagagcttca tgcgcgcgca ggccaggctc tcgcgcagcc ccatgcgcac
     1441 ggtgggcttg agcgagggaa tggcctcgtg cgccgcgcgc aggtggtagt taggaatgcg
     1501 cggcgcgaga tggtggatgt ggtgatagcc gatgttgccg gtgatccagc gcaacacggc
     1561 cggcaggtcg tagaacgagc tgccgtcgac cgccgcggct ttcggatccc agtcgctgcg
     1621 gcgtacccag taactttcct cgaagctgtg ctgaacgtag aacagccaga cgccgagcgc
     1681 gcccgcgatc gccacgaccg gcagatgcgc cgcgagcacc acgccccagc cgagccacac
     1741 ggacagcacg acgccgagcg cgagcagcga gagattgttg agcgccacgc tgcgccattc
     1801 cttcttccag ctccagggca tgccgagcgg aaaccggtgc ttggcgacga actgatagaa
     1861 cggcccgatg ccgagcatca cgggcatgct gcgatagaag cggtaacaga aacgcatcca
     1921 cggtgagctc gcgcggtact cgctgacggt catcgtgcgg atgtcaccga gctcgcgctg
     1981 atcgagattc cccgatgtcg cgtgatgcac gccgtgcgtc ttcttccaat acgcgaacgg
     2041 gaacagcgtg agcagcccaa ggctcgcacc cagccaacgg ttggcgcggg agcttctgaa
     2101 gaacgagccg tgactgcagt cgtgctgaag gatgaagagg cgcacgtaca gcgccgcggt
     2161 cggcagcgcc agcagcaa
//
LOCUS       DATDEU010000310         2911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1222824, whole genome shotgun sequence.
ACCESSION   DATDEU010000310 DATDEU010000000
VERSION     DATDEU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222824"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VIV63_07415"
     CDS             complement(<1..870)
                     /locus_tag="VIV63_07415"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEY6124464.1"
                     /translation="MIDLSRARAWRRAWLCLVPLLGVFSLSMGYAQTQAGRSEAEATM
                     KRATQFMVDKVAYRGGYLWNYLPDMSRRWGEMEARETMIWLQPPGTSSMGHVFLDAYH
                     ATGDDYYYRAAEQVAGALIWGQHPSGGWNYVIDFAGDRSLRDWYATVGKNAWRLEEFQ
                     HYYGNATFDDQTTTDAATFLLRLYMEKLDSRYRAPVERAIQFVLDSQYPIGAWPQRYP
                     LQHEFSHHGKPDYTSFLTFNDDVAAENMDFLLKCYQALGDARMIDPIVRGMNAFLVTQ
                     QGAPNPGWSLQYTL"
     gene            complement(893..>2911)
                     /locus_tag="VIV63_07420"
     CDS             complement(893..>2911)
                     /locus_tag="VIV63_07420"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY6124465.1"
                     /translation="NRGFQACPGAWDHAWSTTIEIVMPRTLVKVLSYLLLAVPVFGAA
                     AEPQFDNPLLRQRADPHAMLHTDGYYYFIATAADWDRIELRRTQDLNALSTAETKVIW
                     RKHGTGPMSAHIWAPEIHYIDGKWYIYFTASRSDAIWEVRPYVLENASPDPFKGEWKE
                     LGRIDTGWDSFSLDGTTFKHKNKRYFVWTQRGRTPEEGRGTNIYIAQMSSPTKITGKV
                     AMLTRPEYEWEKRKYDVNEGPAVLIRNGRVFMTFSASAIDANYCMGLLTASADADLVD
                     AASWKKSPEPVFKSSEATGQYGPGHNSFTTTRDGKTDLLVYHAREYRDIVGSELADPN
                     RNTRAQVLRWKRDGTPDFGVPEADIGKVAAKPLYRDPLFDGAADPVIVHAGSNGRWYM
                     LYTNRRANAQGTKGVEWVHGTRIGIAESMDAGVSWRYIGEADIDLPPQFGGSKATHWA
                     PDVIRDDLGTFHMFLTVVPGIFEDWKHPRHIVHLTSKDLRSWRDAREVRLPTNNPIDA
                     SVVRLPGGGWRMFYNNEADNKAIWFADSPDLENWTDRGKLITDQAGEGPKAFQWNGKS
                     WLITDVWRGLAVYRSDDGLTWARQPKNLLEAPGRGADDQVKGAHADVVVSGGRAWLFY
                     FTHPGRKDSTADTYDTRRSSIQVVELRQDGDWLSADRDAETRVSLRSQ"
BASE COUNT          476 a          921 c          924 g          590 t
ORIGIN      
        1 gagcgtgtac tgcagcgacc agcccgggtt cggcgcgccc tgctgcgtga ccaggaacgc
       61 gttcatgccg cggacgatcg ggtcaatcat tcgcgcatcg ccgagcgcct ggtagcactt
      121 gagcaggaag tccatgtttt ccgcggcaac gtcgtcgttg aacgtgagga acgacgtgta
      181 gtcgggcttg ccgtggtgtg agaactcgtg ctgcagcgga tagcgctgcg gccacgcgcc
      241 gatcggatac tggctgtcga gcacgaactg gatggcgcgt tccaccggcg cgcggtatcg
      301 cgagtcgagc ttttccatgt acaggcgtaa caggaatgtg gccgcgtccg tcgtggtctg
      361 gtcgtcgaag gtcgcattgc cgtagtagtg ctggaactcc tcgagacgcc acgcgttctt
      421 gccgacggtt gcgtaccagt cgcgaaggct gcggtcaccc gcgaaatcga tgacgtagtt
      481 ccagccgccg ctgggatgct ggccccagat cagggcgccc gcgacctgct cggccgcgcg
      541 gtagtaatag tcatcgccgg tggcgtgata cgcgtccagg aacacgtggc ccatgctcga
      601 cgtgcccggc ggctgcagcc agatcatcgt ctcgcgcgct tccatctcgc cccagcgacg
      661 ggacatgtcg ggtagatagt tccagaggta gccgccgcga tacgcgacct tgtcgaccat
      721 gaattgcgtc gcgcgcttca tggtggcttc ggcttcggac ctgccagcct gcgtctgcgc
      781 gtaacccatc gacagcgaaa agacaccgag cagcggaacg agacacagcc atgcgcgccg
      841 ccaggcgcgt gcccgcgata aatcgatcat cgtcgaaccc ccgggttcag atctattggg
      901 agcgcagtga gactcgtgtt tccgcgtcac gatcggcact gagccagtcg ccatcttgcc
      961 tcagctccac cacctgaatc gagcttcgtc gtgtgtcgta cgtatcagcc gtcgaatcct
     1021 tgcggcccgg atgcgtgaaa tagaacagcc aggcccggcc accgctcacc acgacgtccg
     1081 cgtgtgcgcc tttgacctgg tcgtccgcgc cccgtcccgg cgcttcgagc agattcttcg
     1141 gctgtcgcgc ccaggtcagg ccatcgtccg agcggtagac cgcaagaccg cgccagacgt
     1201 ccgtgatcag ccacgacttg ccattccact gaaacgcttt cggaccttcg ccggcctggt
     1261 cggtgatgag tttgccgcga tcggtccagt tctcgaggtc cggactgtcc gcgaaccaga
     1321 tcgccttgtt gtccgcttcg ttgttgtaga acatgcgcca gccgccgccg ggcaggcgca
     1381 cgaccgacgc atcgatcggg ttgttagtcg gcagccgcac ttcccgcgca tcgcgccagc
     1441 tgcgcaggtc cttgctcgtc aggtgcacga tgtggcgcgg atgtttccag tcttcgaaga
     1501 ttccgggcac cacggtgagg aacatgtgga atgtacccag gtcatcgcgg atgacatcgg
     1561 gcgcccagtg cgtcgccttg ctgccgccga actgcggcgg caggtcgatg tccgcttcac
     1621 cgatgtatcg ccagctgacg ccggcatcca tcgattcggc gatgccgatg cgcgtgccat
     1681 ggacccattc cacgcccttt gtgccctgcg cgttcgcgcg ccgattggtg tagagcatgt
     1741 accagcggcc gttggagccg gcgtgcacga tcacgggatc ggcggcgcca tcgaacagcg
     1801 gatcgcgata cagcggcttg gccgccacct tgccgatgtc ggcctcggga acaccgaaat
     1861 ccggtgtgcc gtctctcttc cagcgcagca cctgcgcgcg cgtgttgcga ttcggatccg
     1921 cgagctccga gcccacgatg tcgcggtatt cgcgggcgtg atagacgagc agatcggtct
     1981 tgccgtcacg cgtcgtggta aagctgttgt ggccgggtcc gtattgcccc gtggcctcac
     2041 tactcttgaa caccggttcg ggcgatttct tccacgacgc ggcatcgacc aggtctgcat
     2101 cggcggaagc cgtgagcagc cccatgcaat agttggcatc gatggcgctg gccgagaacg
     2161 tcatgaacac ccggccgttg cggatcagca cggccggccc ttcgttgacg tcgtacttgc
     2221 gcttttccca ctcgtattcg ggacgagtca gcatcgccac cttgccggtg atcttcgtcg
     2281 gcgagctcat ctgtgcgatg tagatgttgg tgccccttcc ctcctccggg gtacgtccgc
     2341 gttgggtcca cacgaaatag cgcttgttct tgtgtttgaa cgtggttccg tcgagcgaga
     2401 agctgtccca gccggtgtcg atccggccga gttccttcca ctcgcccttg aacggatccg
     2461 gggaggcgtt ttcgagcacg tacggccgca cttcccagat cgcgtcgctg cggcttgctg
     2521 tgaaatagat gtaccacttg ccgtcgatgt agtggatttc cggcgcccag atgtgcgcac
     2581 tcatcggacc cgtgccgtgt ttgcgccaga tgaccttggt ttccgcggtg gatagggcgt
     2641 tcaggtcctg cgtgcgccgc agttcgatgc gatcccagtc ggcggcggtg gcgatgaagt
     2701 agtagtagcc atccgtgtgg agcatggcgt gcggatcggc gcgctggcgc agcagcggat
     2761 tgtcgaactg cggttcggcc gccgcgccga agacgggcac ggccagcaac agatacgaca
     2821 ggactttgac cagcgttcgg ggcatgacga tctcgatggt ggtactccac gcatggtccc
     2881 atgcacctgg gcacgcttga aatcctcgat t
//
LOCUS       DATDEU010000311         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1660434, whole genome shotgun sequence.
ACCESSION   DATDEU010000311 DATDEU010000000
VERSION     DATDEU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660434"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /gene="obgE"
                     /locus_tag="VIV63_07425"
     CDS             <1..1005
                     /gene="obgE"
                     /locus_tag="VIV63_07425"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY6124466.1"
                     /translation="RDCFGRGGQDSLVMVPVGTTVRDAETGEVLGDLNKADMELLVAR
                     GGKGGWGNQKFKNSKNRSPRQYGPGLPGEQRTLELELKVIADVGLLGLPNAGKSTLIS
                     AVSAARPKIADYPFTTLYPNLGVISVSDQRSFVMADIPGLIEGAADGAGLGIRFLKHL
                     QRTRVLLHLVDIAPTDPSADPVRDAIAIVGELGKFSEDLLAKPRWLVINKSDLLPPEE
                     AAAKAVSIAVALKYRGPAFLISGATHAGTRDLTEAVMRFIETGEAPAQGNLPLPEIRE
                     PRKPAAKKPAPKKKSAPKKKTAAKKPARKKVTPKKAPTRKKPAPKRKKAAAQKRKSRR
                     "
     gene            complement(1063..1326)
                     /gene="rpsT"
                     /locus_tag="VIV63_07430"
     CDS             complement(1063..1326)
                     /gene="rpsT"
                     /locus_tag="VIV63_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY6124467.1"
                     /translation="MANTKSAEKAARRAETNRNRNVALRSRMRSALRKVVDAVATGNK
                     ADASTALKEAQPVIDSLVNKNVIHRNKAARHKSALTARIKAMK"
     gene            1468..3030
                     /gene="murJ"
                     /locus_tag="VIV63_07435"
     CDS             1468..3030
                     /gene="murJ"
                     /locus_tag="VIV63_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY6124468.1"
                     /translation="MSGAFFKSTAVTGVATLVSRITGLAREMIFAQAFGASAFMDAWL
                     VAFKIPNFLRRLFAEGSFSQAFVPVISEFKIKRSKDEARELVDSVAGTFGVLLLVVTL
                     LGVIAAPLIILAFAPSWHNEPDKWDLATSMLRWTFPYLFFISLSALFSGVLNSYGRFA
                     LPAYTQVIMNVVMIIVALWIAPHSENPGVALAMGVFVSGLLQLGFQVPAVVKLGLFRW
                     PRWRPAAEGVRRIGRLMLPGVFGSSVAQVSLLLDTQIATFLITGSVSWLYYADRLMEF
                     PLGVFSIALATVILPGLSRHHAGESKEHFTHTLDWALKLVVLLVSPAAVAMLVFAGPL
                     TATTFGYGAFLPRDVQMASYALMAYSWGLLGFSLVKVLAPGYFARQDTRTPVRVGLIA
                     LGCNMALNVLVVLPAHYMGFPYPHILLATSTCISAAINTTLLWRGLVRAGVYQPGPGW
                     GVLLARVIFANAVMAALLIWLGGDIAGWLAASPWERAARLTFCVLAGAAAYFAALFVC
                     GVRMRHMRNAST"
     gene            3033..>3370
                     /locus_tag="VIV63_07440"
     CDS             3033..>3370
                     /locus_tag="VIV63_07440"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY6124469.1"
                     /translation="MDFIRGIQGLKPHHRGAAITVGSFDGIHLGHGALIARACALAAH
                     AGKPAIMLTFEPLPREYLSARATPPLEPPARLTDLRERWRVLSHSDLSAVCVLGFNEK
                     LRQLSGEQFV"
BASE COUNT          530 a         1088 c         1088 g          664 t
ORIGIN      
        1 cgcgattgct tcggacgcgg tggccaggat tcgctcgtga tggttccggt cggcaccacc
       61 gtgcgcgacg ccgaaaccgg tgaagtgctc ggtgacctga acaaggcgga catggagttg
      121 ctcgtggccc gcggcggcaa gggcggctgg ggcaatcaga aattcaagaa cagcaagaat
      181 cgatcgcccc gtcagtacgg cccgggcctg cccggcgaac agcgcacgct ggagctcgag
      241 ctcaaggtga tcgcggacgt gggcttgtta gggctgccca acgccggcaa gtcgacgctg
      301 atctccgcgg tctcggccgc ccggcccaag atcgccgact atccgttcac cacgctctat
      361 ccgaacctcg gcgtcatctc cgtgagcgac cagcgcagtt tcgtgatggc cgacattccc
      421 ggtctcatcg aaggtgccgc cgatggcgcc ggcctcggga tccgtttcct caagcatctg
      481 cagcgcacac gcgttctgct gcacctggtg gatatcgcgc ctacggaccc gtcggctgat
      541 ccggtgcgcg acgcgatcgc catcgtcggc gagctcggaa aattcagcga ggacctgctc
      601 gcgaaaccgc gctggctggt gatcaacaag agcgacctgc tgccgccaga ggaagctgcc
      661 gccaaggcgg tgtcgatcgc cgttgctttg aaataccgcg ggccggcttt cctgatctct
      721 ggcgccacgc atgcgggcac gcgtgatctc acggaggccg tgatgcgttt catcgagacg
      781 ggcgaggctc cggcgcaggg caacctgccg ttgcccgaaa tccgcgagcc ccgtaaaccg
      841 gccgcgaaaa agcccgcgcc aaagaagaaa tccgcgccga agaaaaagac tgccgcgaag
      901 aagcctgcgc gcaagaaggt cacgcccaag aaggcgccga cccggaaaaa acccgcgccc
      961 aagcgcaaga aagccgcagc ccagaaacga aaaagccggc gttagccggc tgtttcgtgc
     1021 tccacgccaa ctcctttgca ggaggggcgc cgaaggcttg tcttacttca tcgccttgat
     1081 gcgggcggtc agcgcgctct tgtggcgggc ggccttgttg cgatgaatca cgttcttgtt
     1141 gaccagcgag tcgatgaccg gctgggcttc cttgagcgcc gtggacgcat cggccttgtt
     1201 gccggtagcg acggcgtcga cgaccttgcg cagggcagag cgcatacgtg aacgcagcgc
     1261 gacgttgcga ttgcggttgg tttcagctcg acgggcggct ttttcggccg acttggtatt
     1321 cgccacgaag caatctccag caattcaggg tcgttttgag gccgcgtagt gtgatgaccg
     1381 acccctacct tgtcaacgct tcatcagcca gtccggtata gtgcgcgcct tgcgcagttt
     1441 ttcctttatt tttcggggcc ttgcagggtg agtggagcgt ttttcaagag caccgccgtg
     1501 accggggtgg cgacactggt gtcgcgtatc accggcctgg cccgcgagat gatctttgcc
     1561 caggcctttg gcgccagcgc cttcatggac gcctggctgg tggccttcaa gatcccgaac
     1621 ttcctgcgcc gcctgttcgc cgaaggctcg ttctcgcagg ccttcgtacc tgtcataagc
     1681 gaattcaaaa tcaagcgctc caaggacgag gcgcgcgaac tcgtcgattc ggtcgccggc
     1741 accttcggcg tgctgctgct cgtcgttacc ttgttaggcg tcattgccgc gccgctcatc
     1801 atcctggcgt tcgcgccgag ctggcacaac gagcccgaca agtgggacct ggcgacgtcg
     1861 atgctgcgct ggacgttccc gtacctgttc ttcatctcgc tgagcgcatt gttctccggc
     1921 gtactgaaca gctatggcag gttcgcgctg ccggcgtaca cgcaggtgat catgaacgtg
     1981 gtcatgatca tcgtggcgct gtggatcgcg ccgcattccg aaaatcccgg cgtggcgctc
     2041 gccatgggcg tgttcgtcag cggcctgctg caattgggat tccaggtgcc ggcggtcgtg
     2101 aagttaggcc tgtttcgctg gccgcgctgg cggccggccg cagagggcgt gcggcgaatc
     2161 ggccggctga tgttgcccgg cgttttcggt tcttcggtcg cgcaagtcag cctgctgctc
     2221 gacacgcaga tcgccacgtt cctgatcacc ggcagcgtct cgtggctgta ctacgcggac
     2281 cggctcatgg agtttccgct cggcgtattc agcattgcgc tcgccaccgt gatccttccc
     2341 ggcctttccc ggcatcacgc cggcgagtcg aaggaacact tcacgcacac gctcgactgg
     2401 gcgttgaaac tggtggttct gctcgtgtcc ccggccgcgg tggcaatgct ggtgttcgcc
     2461 ggtccgctga ccgccaccac gttcggctac ggcgcattcc tgccgcgcga cgtgcaaatg
     2521 gcgagttacg cgctcatggc ctactcatgg ggcttgctcg ggttcagcct ggtgaaggtc
     2581 ctggcgcccg gatacttcgc gcgccaggac acgcgcacgc cggtgcgggt cgggctgatc
     2641 gcgctcggct gcaacatggc gctcaacgtg ctcgtcgtgc tgcctgccca ctacatgggc
     2701 ttcccctatc cgcacatcct gctcgccacc tcgacctgca tctcggccgc catcaacacc
     2761 acgctgctct ggcgcgggct ggttcgtgcg ggcgtctacc agccagggcc gggttggggc
     2821 gtgttgcttg cgcgagtgat cttcgcgaac gccgtgatgg ccgcgttgct catctggctc
     2881 ggtggtgaca tcgcaggctg gctggcggca tcgccgtggg aacgcgcggc gcgactgacc
     2941 ttctgcgtgc tggccggcgc cgccgcctac tttgcggcgc tgttcgtttg cggcgtgcgc
     3001 atgcgccaca tgcgcaacgc gagcacctga ccatggattt catccgtgga atacagggtt
     3061 tgaagcccca tcatcgggga gcggccatca cggtcggcag cttcgatggc atccacctcg
     3121 ggcatggcgc gctgattgcg cgtgcctgcg ccctggcggc gcatgccggc aagcccgcga
     3181 tcatgctgac gttcgagccg ctgccgcgcg agtacctgtc cgcccgggca acgccgccgc
     3241 tggagccgcc cgcgcggctc acggatctgc gcgagcgctg gcgcgttctc tcgcattcgg
     3301 acctgtccgc ggtctgtgtt ctgggcttca acgagaagtt gcggcagcta tcgggggagc
     3361 agttcgttaa
//
LOCUS       DATDEU010000312         3194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1806508, whole genome shotgun sequence.
ACCESSION   DATDEU010000312 DATDEU010000000
VERSION     DATDEU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806508"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VIV63_07445"
     CDS             <1..875
                     /locus_tag="VIV63_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY6124470.1"
                     /translation="PAVHEMLSSKHPSAANYLSSFDEKWTACRQAALDHDLVTWPVAP
                     VRYVPIPAHAREAAASLYYLNYRSPAPFDSHAMFDYVVPAIEGLTGDALEGRLRIMND
                     SAITLNHVVHHGAIGHHVQNHHAYAGKSRVGRVAAVDTANRIAMFSGGSLAEGWACYV
                     CDLMEEIGFLTPLERIAQQHTRVRIAARAVADLSIHTGAITLPQAARLYADRAYLSEA
                     AARGEAVRNTMFPGTAVMYWLGTRGLHRLRASMRSRQGEEFSLRAFHDRVLSYGATPV
                     MLIAKLMLAEEAAS"
     gene            872..2515
                     /locus_tag="VIV63_07450"
     CDS             872..2515
                     /locus_tag="VIV63_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6124471.1"
                     /translation="MIRPSLRVLVVASAVVAVAAACGSGSETATSSAPAASPSTRATR
                     LVVGYDREPDTLNRFSTHILEDIETCVVEGLVTTDESMTIVPLLAAAIPTIDNGGVVL
                     RQDGGMDVTWRLRPGVKWHDGTPHTSADVKFTVEAINSPAYNPESTDGFDRISSVDTP
                     DPLTAVLHYREVYAPYALQFVRGTLPKHLLEGRDIDSANDYNRGPLGTGPYRVAEWKS
                     GEYIRLEAVPNYWRGAEVPAIQTLLFKFIANTNTRINQLKSGEVDMVVMFPWDKHREV
                     AAIPGVTVHRINGNGYEHVTLNQRALPAFADVRVRQALTHAIDRPLISTTILEGLAPV
                     THGPVQPVSWAHNPNVRTYGFDPVRAKALLDEAGWRDSDGDGIRDQGGRRFSFTLITQ
                     AGFAVRENVAQVLQRQFRDVGIEAAVQLHDGTSISKLWFEGRFDAMLHWWQLPADPEL
                     TLFFAKDRMPPRGRNINYVADDALTELVYQADRTVNQSERKRILGEAQLRIADLAVEI
                     PLYGVTKLDAVPTRLRGFTGNPTNTGPFWNVHAWTLTEP"
     gene            2494..>3194
                     /locus_tag="VIV63_07455"
     CDS             2494..>3194
                     /locus_tag="VIV63_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6124472.1"
                     /translation="MDVDGTVTSGFAANSRVGPLIGLAARRAAEAVPLLFLVSGIAFG
                     LLQLVPGGPLEVYLSNPGVRAEDLERLRRALGLDRSVGEQYVSWLAAFVRGDWGFSFS
                     DGRPVFDRIVERLPASIELLAASLVLALSAAIPAGIVAALTRRSVIDRSISTVAAVGI
                     SMPTFWLGLMLQLTFTIALGWLPSSGRGADSTGIARLQYLIMPAAVLAFAHAAAWSRY
                     LRASLIDVLNAPFMAA"
BASE COUNT          519 a         1064 c         1055 g          556 t
ORIGIN      
        1 ggccggccgt ccacgaaatg ttgtcgtcga agcacccgtc ggcagcaaac tacctgtcgt
       61 ccttcgatga gaagtggacg gcgtgccggc aggccgcact cgaccacgac ctcgtgacct
      121 ggcccgtcgc gccggttcga tacgtgccga ttcccgcgca tgcgcgcgag gctgccgctt
      181 cgctttacta tctcaactac cgatcgccgg cgccgtttga ttcgcacgcg atgttcgact
      241 acgtcgttcc cgcgatcgag gggctgaccg gtgacgcgct cgaggggcgc cttcgcatca
      301 tgaacgacag cgcgatcacg ctcaatcacg tcgtacatca cggagcgatc ggtcatcacg
      361 tgcagaacca tcacgcgtat gcgggaaagt cgcgcgtcgg ccgggtggcc gccgtcgata
      421 cggcgaaccg cattgcgatg ttttcgggcg gcagcctcgc cgaggggtgg gcgtgctatg
      481 tctgtgacct gatggaagag atcggatttc tgacgccgct cgagcgcatc gcgcagcagc
      541 acacgcgcgt gcgcatcgcc gccagggcgg tcgccgactt gtccattcac accggtgcta
      601 tcaccctgcc gcaggccgcg cgtctgtatg ccgatcgggc gtacctgagc gaggcggcgg
      661 cccgcggcga agccgtgcgc aacaccatgt tcccaggcac ggcggtgatg tactggctgg
      721 gcacgcgcgg actgcatcgc ctgcgcgcct cgatgcggtc gcggcagggc gaggagtttt
      781 cgctgcgcgc gtttcacgat cgcgtgctgt cctatggagc gactccggtg atgctgatcg
      841 cgaagttgat gcttgctgaa gaggcggcgt cgtgatccga ccatcgttgc gggtcctcgt
      901 cgtggcgagc gcggtcgtcg ccgtggccgc cgcgtgcggc agcgggtcgg agacggccac
      961 gtcgagcgca ccggcggcgt cgccatcaac gcgcgcgact cgactggtcg tcggctacga
     1021 ccgcgagccc gacacgctca atcgcttcag cacgcacatt cttgaagaca tcgagacctg
     1081 cgtggtggag ggactggtca cgaccgatga gtcgatgacc atcgtgccgc tgctggcggc
     1141 ggccattccg acgatcgaca acggtggggt cgtgctgcgt caggacggcg gcatggacgt
     1201 gacgtggcgg ctgcgccccg gcgtcaagtg gcacgacggc acgccgcaca cctccgccga
     1261 cgtgaaattc acggtcgagg cgatcaacag ccccgcttac aaccccgaaa gcaccgacgg
     1321 gttcgatcga atcagcagcg ttgacacgcc tgacccgctg accgcggtgt tgcactaccg
     1381 cgaggtctac gcgccgtacg cactgcaatt tgtccgcggc acactgccaa agcacctgct
     1441 cgaaggccgc gacatcgata gcgcgaacga ctacaatcgc gggccgctcg gcactgggcc
     1501 gtatcgcgtc gccgagtgga agagtggcga atacatccgg ctcgaagcgg tgccgaatta
     1561 ttggcgcggc gcggaagtgc cggcgatcca gacgctgctc ttcaagttca tcgccaacac
     1621 caacacccgc atcaatcaac tgaagagcgg cgaagtcgat atggtggtga tgttcccctg
     1681 ggacaagcat cgcgaggtcg ccgccatccc cggcgtgacc gtgcatcgta tcaacggcaa
     1741 cggctacgaa catgtcacgc tcaaccagcg cgcgctgccg gcattcgccg acgttcgcgt
     1801 ccgccaggcc ctgacgcacg ccatcgaccg gccgctcatc tcgacgacca tcctcgaggg
     1861 gctggcgccc gtcacgcacg gcccggtgca gccggtgtcg tgggcacaca accccaacgt
     1921 gcgtacctac gggttcgatc cggtccgcgc caaggcgctg ctggacgagg ccggctggcg
     1981 cgactccgac ggcgacggga ttcgcgacca gggtggcagg cgcttctcgt tcacgctcat
     2041 cacgcaagcg ggtttcgccg tccgcgagaa cgttgcccag gtgctgcagc gccagttcag
     2101 ggacgtcggc atcgaggccg cggtgcaact gcacgacggc acgagcatca gcaagctgtg
     2161 gttcgagggc cgcttcgacg cgatgctgca ctggtggcaa ctgcccgcgg atccggaact
     2221 gaccctgttc ttcgccaagg atcggatgcc gccgcgcggc cgcaacatca actacgtcgc
     2281 agacgatgcg ctgacggagc tggtctacca ggccgataga acggtgaatc agtccgagcg
     2341 aaagcgcatc ctcggcgaag cgcagctgcg catcgccgac ctggcggtcg agattcctct
     2401 ctacggagtg accaagctcg atgcggtgcc aacccggctg cgcggtttca cgggcaatcc
     2461 caccaacacc gggcccttct ggaacgtgca cgcgtggacg ttgacggaac cgtgacgagc
     2521 gggttcgctg cgaactcgcg cgttggtccg ctgatcggcc tggccgccag acgcgcggcc
     2581 gaggcggtgc cattgctgtt cctcgtgtcc ggcatcgcgt ttggcctgct gcaactcgtc
     2641 cctggtggac cgctcgaagt ctatctgtcc aaccccgggg tgagggccga ggacctcgag
     2701 cggctgcgac gcgcgctcgg cctcgatcga tcggtcggcg agcagtacgt cagctggctg
     2761 gccgcgttcg tgcgcggcga ctgggggttc agcttcagcg acggccggcc cgtcttcgat
     2821 cgcatcgtcg aacgcctgcc cgcgtcgatt gaattgctgg cggcatcatt ggtattggcg
     2881 ctttcggccg ccataccggc gggcattgtc gcggccctga cgcgtcgctc cgtgatcgac
     2941 cgctcgattt caaccgtcgc cgcggtcggc atatcgatgc cgacgttctg gcttggcctg
     3001 atgctgcagc tcacgtttac gatcgcgctc ggctggctgc cgtcgtcggg ccgaggcgcc
     3061 gacagcaccg gcatcgcgcg cctgcagtat ctgatcatgc cggcggcggt gctggcgttt
     3121 gcccatgcgg ccgcgtggtc gcgctacttg cgcgcctcgc tcattgacgt actgaacgcg
     3181 ccgttcatgg cggc
//
LOCUS       DATDEU010000313         3988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1806586, whole genome shotgun sequence.
ACCESSION   DATDEU010000313 DATDEU010000000
VERSION     DATDEU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806586"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VIV63_07460"
     CDS             complement(<1..398)
                     /locus_tag="VIV63_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6124473.1"
                     /translation="MADRIECVVIGAGVVGLAVARALALAGREVLLLEAETHAGTITS
                     ARNSGVIHAGLYYTPGSFKARFCVAGNRALYAYCQARGVDHHNTGKLIIANGSEEEKV
                     LLHLLERARTNGVEGVRMVSATEAHELEPEV"
     gene            413..1048
                     /locus_tag="VIV63_07465"
     CDS             413..1048
                     /locus_tag="VIV63_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124474.1"
                     /translation="MQTSLIRVLAALALIAVASCSGTSGNPYAAIQNPPLLQARLHTV
                     TLATDSANAAEALQVKGYQASQFASNYPVSNSVEATLWGVPLPAVEGVTHFRSPNAAD
                     PGVRVVKMAMAARGSKPDAKVEKAFFRNVLGSDVPQWPAGIERNDGIRVQVWTFVISD
                     VMAANKSLRENGVPVVFDPVSITTAYLGDHKTMAIRAPDGAIVELVQTAAQ"
     gene            1144..>3988
                     /locus_tag="VIV63_07470"
     CDS             1144..>3988
                     /locus_tag="VIV63_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY6124475.1"
                     /translation="MDRCRYVAAFAVSVGVALALSLTACGGGDDAPPEAPPPVVTPPP
                     PGTLIGAAGGTVTGPAGTSVVIPAGALASEVRILIEQSTTGAPPLPSGFSLAGQTFAL
                     TPHGTTFATPVTVTLPFNPATVPAGVTPVFYKTTNNQTVWQEIAGAVFGPSSVSVQVT
                     SFSIAGVVLPPLSAGLPSREWHANILRGDALVEEEIAHDGSTSGPLNLIFDFGGILPW
                     PGGVSVPLDGFAVGDITASEDGADWSLGTESPTGFGGVVNDPIGSSVDYKQTMSFHKR
                     TENATLSITFKEAFLETVDANGILGRGCPGVRRNGLACDMILAELYVHVEAFLVPEDI
                     DPDQPFDVFFRVAGGATLMGIAGSWSSNASTARESWVPLWNVEDFDFTVDALGEHEEA
                     HITMQLRAPRTIQVDLSSVPTGQPFTLQSFAFVRAYNRAVAASEFATGARAFLRDPLS
                     FEGATVVHTGLDVIEPVESDGPNDAPVTPAACTPGPAPDPSAGTLQFAAANYTQLESN
                     GTPLITVTRTGGTRGAVTATISSSDGTAVAGVDYTALNTSVFFADGDATPRTLAVRVT
                     DNNAHSEPDKTVNLTLSQPGGCAAIGTPATTVVTLRDDDAPPPPPTFTISGTVSGLVG
                     TGLVLQDLHFRPITPGNGAFTMPSPTRVGDPFAVTVLTQPINPVQVCTVVNGSGTMPN
                     APVTNVLVNCVTPQVSGALDLGFGGTGKVSSPFGGDDTRMVLQPDGKIIMVGGNAGTG
                     SNFLIARYDTDGSLDESFGGAGTGFTTTDIAGGADEALGVALQSDGRIIVVGSARVGS
                     NDDFAVVRYDTNGVLDATFGTLGKVTTDFAGDRDRAFAVAVQPDDRIVVVGDAIMPAP
                     GNTDFAVARYTANGAPDPTFDGDGRLTADIGGGVDIAQSVLLQGNQGAILVSGVLTLG
                     NSPSLEHGGIARFTSSGALDTSFATAGKRTFPSL"
BASE COUNT          665 a         1366 c         1297 g          660 t
ORIGIN      
        1 acttcgggct cgagctcatg ggcctcggtg gccgagacca tgcgtacacc ttcgacgcca
       61 ttggttcgtg cgcgttcgag cagatgcaat agcaccttct cctcttcgct gccgttggca
      121 atgatgagct tgccggtgtt gtgatgatcg acgccgcgcg cctggcagta ggcatacagg
      181 gcgcggttgc cggcgacgca gaagcgcgcc ttgaagctgc cgggcgtgta gtagagcccc
      241 gcgtgaatga cgccgctgtt gcgcgccgag gtgatggtgc ccgcgtgtgt ctccgcctcg
      301 agcagcagga cttcgcgacc ggccaatgcc aatgcacgcg cgacggcgag gcccaccacg
      361 cccgcaccga tgacgacgca ttcgatcctg tctgccatat actcaattta tcatgcaaac
      421 atccctgatc cgcgtcctcg cggcgcttgc gctcatcgcc gtggcatcct gcagcggcac
      481 ctccggcaac ccgtatgcgg ccatccagaa tccgccgctg ctgcaggccc ggctgcacac
      541 cgtgaccctg gcgaccgaca gcgcgaatgc cgccgaggcg ctgcaggtga agggctacca
      601 ggccagccag ttcgcctcta actatcccgt ttcgaattcc gtcgaagcca cgctctgggg
      661 cgtgcccctg ccggcggtcg aaggggtgac ccacttcagg tcgccgaatg ccgcggaccc
      721 gggcgttcga gtggtgaaga tggcgatggc ggccaggggc tcgaagccgg atgcgaaagt
      781 cgagaaggcg tttttcagga acgtgctggg cagcgacgtg ccgcagtggc cggccggcat
      841 cgaacggaac gatggcatcc gcgtccaggt gtggaccttc gtgatttccg acgtgatggc
      901 ggctaacaag agcctgcgcg aaaacggtgt gccggtggtg ttcgatccgg tcagcatcac
      961 caccgcctat ctcggcgatc acaagaccat ggccatccgc gcgccggacg gcgccatcgt
     1021 cgagctggtg cagaccgcgg cgcagtagcc gcgaaaactc ccgtgcgggc gttgtcccct
     1081 tcgggcggca aaagccgtta gcactcctgc ggggccattt gcgtaccccc agcaggagtt
     1141 cacatggatc gttgcagata tgtggccgcg ttcgcggtgt ccgtcggcgt tgcgctggcg
     1201 ctttcactca cggcgtgtgg aggcggcgac gatgccccgc ccgaggcgcc gccacccgtg
     1261 gtcacgccgc cgccgcccgg aacactgatc ggtgccgccg gcggaacggt gacgggtccc
     1321 gcgggtacga gcgtggtcat tcctgccggc gcgctcgcat ccgaagtgcg catcctcatc
     1381 gaacagagca cgaccggtgc gccgccgttg ccgagcggct tctccctcgc gggacagacg
     1441 ttcgcactca cgccgcatgg aaccacgttc gcgacgcccg tgacggtgac gttgccattc
     1501 aatccggcca cggtgccggc gggcgtcacg ccggtgttct acaagaccac caacaaccag
     1561 accgtctggc aggaaatcgc gggcgcggtc ttcgggccat cgagcgtgag cgtgcaggtg
     1621 acgagctttt cgattgcggg cgtcgtcctg ccgccactgt cggccggtct gccgtcgcgg
     1681 gagtggcacg cgaacattct gcgcggcgac gcgctggtcg aagaagagat cgcgcacgac
     1741 ggatcgacct ccggcccctt gaacctgatc ttcgacttcg gcggaatcct tccctggccc
     1801 ggcggcgtat ccgtgccgct cgacggcttc gcggtgggtg acatcacggc gtcggaggat
     1861 ggcgcagact ggtcgttggg caccgaatct cccaccgggt tcggcggcgt cgtcaacgat
     1921 ccgatcggca gctccgtcga ctacaagcag acgatgagtt ttcacaagcg gaccgagaac
     1981 gcgacgctct cgatcacgtt caaggaagcc tttctcgaga cagtggacgc caacggcatc
     2041 ctcggccgcg gatgtcccgg cgtgcgccgc aacggcctcg cctgcgacat gatcctcgcc
     2101 gagctctacg tgcacgtcga agctttcctg gttcccgaag acatcgatcc cgaccagccg
     2161 ttcgacgtgt tctttcgcgt cgcgggcggc gccacgctca tgggtatcgc cggatcgtgg
     2221 agcagcaatg cgagcactgc gcgggaatcg tgggtgccgc tgtggaacgt cgaggacttc
     2281 gacttcacgg tcgacgcatt gggcgaacac gaagaggccc acatcacgat gcaattgcgc
     2341 gcaccgcgca ccatccaggt ggacctgtcg tcggtcccga cgggtcagcc cttcacgctg
     2401 caatcattcg cattcgtgcg cgcctacaac cgcgccgttg ccgccagcga gttcgccacc
     2461 ggagcgcgcg cgttccttcg cgacccgctg agcttcgaag gcgcgaccgt ggttcatacg
     2521 gggctcgacg tcatcgagcc ggtcgaatcg gacggtccca acgacgcgcc cgtgacgccc
     2581 gccgcgtgca caccgggtcc ggcccccgat ccgtcggccg gcacgctgca gttcgccgcg
     2641 gctaactaca cacagctcga atcgaatggg acgccgctca tcacggtcac caggaccggc
     2701 ggaacgcgcg gcgccgtgac agccaccatt tcctcgagcg acggcacggc cgtggccggc
     2761 gtggactaca cggcgctcaa tacctcggtg ttcttcgccg atggcgacgc gacaccgcga
     2821 accctggccg tacgcgtcac cgacaacaac gcgcactccg agcccgacaa gaccgtgaat
     2881 ctcacgctct cgcagcctgg cggttgcgcg gcgatcggca cgccggccac cacggtggtg
     2941 acgctgcgcg acgacgatgc gccgccacca ccgccgacct tcacgatcag cggcacggtc
     3001 agcgggctcg tcggaaccgg gctcgtcctg caggacctgc atttccggcc gatcacgccc
     3061 ggcaacggcg cgttcacgat gccttcgccc acgcgtgtcg gcgacccctt cgcggtgacc
     3121 gtgctgaccc agccgatcaa ccccgtgcag gtctgcactg tggtgaacgg cagcggcacg
     3181 atgccgaacg cgcccgtcac caatgtgctg gtgaactgcg tgacgccgca ggtcagtggc
     3241 gcgctcgatc ttggcttcgg cggcaccggc aaggtgagca gccccttcgg cggggacgac
     3301 actcgcatgg tgctgcagcc cgatggcaag atcatcatgg tgggcggcaa cgcgggcacg
     3361 ggaagcaatt tcctgattgc gcgttacgac accgatggca gtctcgacga atccttcggc
     3421 ggtgccggca ccggcttcac gacgaccgac atcgccggtg gcgcggatga agcactgggc
     3481 gtggcgctgc agagtgacgg acgcatcatc gtggtcggca gcgcgcgcgt cggcagcaac
     3541 gatgacttcg cggtggtgcg ctacgacacc aacggtgttc tcgacgcgac gttcggcaca
     3601 ctcggcaaag tcaccacgga tttcgcgggc gaccgcgatc gtgcgtttgc cgtcgccgtg
     3661 cagcccgacg atcgcatcgt ggtcgtgggc gacgcgatca tgcccgcgcc cggcaacacc
     3721 gacttcgcgg tggcgcgtta caccgccaac ggcgcgccgg atccgacgtt cgatggagac
     3781 gggcgcctga ccgccgacat cggcggcggc gtcgacatcg cgcagagcgt gctgctgcag
     3841 ggaaaccagg gcgccattct cgtctccggc gtgctcacac tcggcaattc gccgtcactc
     3901 gaacatggcg gcatcgcgcg cttcacctcg agtggcgcac tcgacacgag cttcgccacg
     3961 gcgggcaaac gcacgttccc cagcctgt
//
LOCUS       DATDEU010000314         5617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_201526, whole genome shotgun sequence.
ACCESSION   DATDEU010000314 DATDEU010000000
VERSION     DATDEU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201526"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VIV63_07475"
     CDS             <1..831
                     /locus_tag="VIV63_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY6124476.1"
                     /translation="VAVVTRPFEMEGNKRARAADSGIAELSKYVDSLITIPNQKLLTV
                     LGAKTTLLDAFKSANHVLQGAVQGIAELITRPGLINVDFADVRTVMSETGMAMMGSGS
                     ARGENRAKEAAEMAVSSPLLEDINLSGAHGILVNVTAGMDLSIGEFQEVGNIVKQFAS
                     DDATVVVGTVIDPELSDEMRVTVVATGLGKPEIAVSGRSPFGRDVPTLSKAVPQAAAP
                     AAEPAMRVVPRRPLTAADYAALDKPAVQRQRAVGDGLHASAESEDVLDIPAFLRRQAD
                     "
     gene            989..1915
                     /gene="lpxC"
                     /locus_tag="VIV63_07480"
     CDS             989..1915
                     /gene="lpxC"
                     /locus_tag="VIV63_07480"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY6124477.1"
                     /translation="MLGQRTLKNSIRAQGVGLHTGQKVLMTLRPAAPDTGIVFRRTDL
                     NPPAEVRAHALNVGDTQLGTTLINGEARVSTVEHLLSAFAGLGIDNAHVDVSAPEVPI
                     MDGSAGPFVFLLQSAGFEDQRTPKKFVRIKQRVRVEDGDKWAEFRPFDGFKVNFEIEF
                     NHPLFKRRGNKASMDFSTTSFLKEVSRARTFGFMRDLEMLRARNLALGGNFDNAIVLD
                     DFRVLNEDGLRYEDEFVKHKILDAIGDLYLLGHSLIGEFSGYKSGHKLNNNLLRKLIS
                     EPQTWEAVTFEKPADAPIAYVQPPPVAAGSAI"
     gene            2041..2613
                     /locus_tag="VIV63_07485"
     CDS             2041..2613
                     /locus_tag="VIV63_07485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY6124478.1"
                     /translation="MDSGNGEKLNRRALLAGAVFLLGGAAALSRFTRQPADGADADGP
                     VLSADQFALLEQISETMVPATDTAGAIAAGVPAFIREMLSAWGSRATRAEIAQVLEAI
                     EQNAWQKFGMNFLEMPAERRLAVLKEFDATRIAAHDGAYRKFKWLVLIGYYHSEVGAT
                     HELRFELVPGAWRACLPITEVGRASAFYGH"
     gene            2619..4313
                     /locus_tag="VIV63_07490"
     CDS             2619..4313
                     /locus_tag="VIV63_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY6124479.1"
                     /translation="MDFDAIVIGSGISGGWAAKELCERGLRTLLIERGRHVTHKSDYQ
                     DFAAPWELSHRGQVPEDEARRDYAVQSTCYAFNSATKQWWVKDSEHPYLTPEGREFLW
                     LRGYHLGGRSLTWARQTYRFSDIDFGANQKDGHGVDWPIRYADVSPWYDRVETFAGIS
                     GNSEGLEQLPDGRFLKPMELNCAELDFKRKLEGSHPSRRVTIGRCANLTEPTPEHIAL
                     GRGPCQLRSVCERGCGYGGYFSTLSATLPAAQRTGNLTVVTDAIVERLDYDNAKRRIS
                     AVRVIDAKTREHRSYQARVVFLCASTIPTAQVLLNSRSDFFPNGLANRSDQVGRNLMD
                     HVHGIGAIGNVPGFLDRYYYGRRPTGLYIPRYANVTEDGRGEYTRGWAFQGKGERPNW
                     DRALDMPSVGAELKTELREPGPWQMVLFGFAEMLPRPDNRVTLHESRTDAAGMPLVHI
                     DCTFGENERKLAAAADRDALAMLTTAGCTNVRALFPHLSPPGTSVHEMGTARMGHDPA
                     TSVLNRHNQAHDVPNLFITDGSCMTSSSCVNPSLTYMALSARAAHYAADRLASGEL"
     gene            4800..>5617
                     /locus_tag="VIV63_07495"
     CDS             4800..>5617
                     /locus_tag="VIV63_07495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY6124480.1"
                     /translation="MNIIFFSRREGRARHFNLAHPFAVAAFAAVLVAILATAFSLGFK
                     IGESKAPPRGVASWTATLAEQQSEIKELKAELQRRVDAVAMRIGQMNAHVIRLDALGK
                     RLTQMANIDNREFDFESQPAIGGPESDIGAAAMQAPDLNSLLNGLEQKISSRDAQLAA
                     LENALLSKKLDEQIRPDGRPVRAGHISSYFGERQDPFNGHQAFHKGIDFASPKGSDVV
                     AVAAGVVTFAGEKAGYGNLVEVSHGNGLTTRYGHNQGLAVGVGRTVARGDTLAFV"
BASE COUNT         1023 a         1887 c         1740 g          967 t
ORIGIN      
        1 gttgcggtcg tgacccgtcc cttcgagatg gaaggcaaca agcgggcgcg cgcggccgac
       61 agcggcatcg cggaactgag caagtacgtc gactcgctga tcaccatccc caaccagaag
      121 ctgctcacgg tgctcggcgc caagaccacg ttgttagacg ctttcaagag cgccaaccac
      181 gtgttgcagg gcgcggtgca gggcatcgcc gagctgatca cgcgaccggg tctcatcaac
      241 gtggacttcg ccgacgtgcg taccgtcatg agcgagaccg gcatggcgat gatgggttcg
      301 ggatcggcgc gcggcgagaa ccgcgcgaag gaagcggccg agatggccgt ctcctcgccg
      361 ctgctcgagg acatcaacct gtcgggtgcg cacggcatcc tggtcaacgt caccgcgggc
      421 atggacctgt cgatcggtga attccaggaa gtcggcaaca tcgtcaaaca gttcgcgtcg
      481 gatgacgcca cggtagtcgt gggaaccgtc atcgaccctg agctgtccga tgaaatgcgg
      541 gttacggtgg tcgccaccgg actcggcaag cccgaaatcg cggtcagcgg ccgttcgccg
      601 ttcggacgcg acgttcccac cctgtccaaa gcggtaccgc aggcggcagc gccggcggcg
      661 gaacccgcca tgcgcgtcgt tccgcgccgt ccgctgacgg cggctgacta cgccgcgctg
      721 gataagccgg cggtacagcg tcaaagggca gtgggtgacg gactccatgc gagtgcggaa
      781 tccgaagatg tgctggatat tccggccttt ctgcggcgtc aggctgactg acgccggaga
      841 ctgattaagg caactgtgtt acggtagcgc cccttttggg ggcacggtgc cgcaggcgac
      901 tgcggccgct ccccgatgcc cccaacgttt cgcgacgaag gtggcgctac ccgagcaccg
      961 tgttaacggc agcgggagat agtttcagat gctcggacaa cgaactctca aaaactccat
     1021 ccgcgcccaa ggtgtcggtc tgcataccgg tcagaaggtc ctcatgacct tgcggcccgc
     1081 ggccccggac accggcatcg tcttccgtcg taccgacctg aatcctccgg ccgaggtgcg
     1141 cgcccacgcg ctgaacgtcg gcgacaccca gctcggcacc actcttatta atggtgaggc
     1201 ccgcgtctcc acggtggagc acctgctgtc cgccttcgcc ggcctcggta tcgacaatgc
     1261 gcacgtggac gtcagcgcac ccgaagttcc catcatggac ggcagcgccg gtccgttcgt
     1321 attcctgctg cagtcggcgg gcttcgaaga tcagcgcacc ccgaagaaat tcgtgcgcat
     1381 caagcagcgc gtgcgcgtcg aagacggcga caaatgggcg gagttccggc ccttcgacgg
     1441 cttcaaggtc aatttcgaaa tcgagttcaa ccatccgctg ttcaagcggc gcggcaacaa
     1501 ggcctcgatg gatttttcga ccacttcctt cctcaaggaa gtgtctcgcg cccgcacttt
     1561 cggtttcatg cgtgatctcg agatgctgcg cgcgcgcaat ctcgcgctcg gcggcaattt
     1621 cgacaacgcc atcgtgctcg acgatttccg cgtgctcaac gaagacggcc tgcgctacga
     1681 agacgagttc gtgaagcaca agatcctcga tgcgatcggc gatctctatc tactcgggca
     1741 cagcctcatc ggcgaattca gcggttacaa gtccggccac aagctgaaca acaacctgct
     1801 gcgcaagctc atttccgagc cccagacctg ggaggcggtc acgttcgaga agcccgccga
     1861 cgcacccatc gcctacgtcc agccgccgcc agtcgcagcc ggctccgcca tctaggcggc
     1921 aattccttgc cgcgcctgta gtcccttttc catgccgccc cggactttga accggcgcac
     1981 gcattcaaaa ttcagctcga agcaacttcg ccgatagaac ggggcggagg aacttacctg
     2041 gtggactcgg gaaatggcga aaaactgaat cggcgtgcct tgctggcggg tgccgtgttc
     2101 ctgctgggcg gggcggccgc gctgtcgcgt ttcacgcggc agcccgcgga cggcgccgat
     2161 gccgatgggc cggtgctctc cgccgatcaa ttcgcattgc tcgaacagat cagcgagacc
     2221 atggtcccgg ccaccgacac cgcgggcgcc atcgccgccg gcgtgccggc cttcattcgc
     2281 gaaatgctct ccgcgtgggg atcgcgcgcc acgcgggccg agatcgcgca ggtgctcgaa
     2341 gccatcgagc agaatgcatg gcagaagttc ggcatgaact ttctggagat gcccgcggaa
     2401 cgccgtctcg ccgtgctcaa ggaattcgat gccacgcgca tcgccgcgca cgacggcgcc
     2461 tatcgcaaat tcaaatggct ggtgctcatc ggctactacc actccgaagt cggcgcgacc
     2521 cacgagctgc gcttcgagct ggtccccggc gcctggcgcg cgtgcctgcc gatcaccgaa
     2581 gtcggccgcg cttcggcgtt ctacggacac tgaagccgat ggatttcgat gccatcgtca
     2641 tcggctctgg aatcagcggc ggctgggccg cgaaagagct gtgcgagcgt ggcctgcgca
     2701 cgctgctcat cgaacgcggc cggcacgtca cgcacaagtc cgactaccag gacttcgccg
     2761 cgccgtggga gctcagccat cgcggccagg tgccagagga cgaggcgcgc cgcgactacg
     2821 cggttcaaag cacctgctac gcgttcaact ccgccaccaa acaatggtgg gtgaaggaca
     2881 gcgaacatcc gtatctcacg cccgaaggac gcgagttcct ctggttgcgc ggctatcacc
     2941 tcggcggccg ttcgctgacc tgggcgcgcc agacctatcg attcagcgac atcgacttcg
     3001 gcgccaacca gaaagacggt catggcgtcg actggccgat tcgttatgcc gacgtttcgc
     3061 cgtggtacga ccgtgtcgaa actttcgcgg gcatttcggg caacagcgaa ggcctcgagc
     3121 aattgcccga cggccgattc ctcaagccga tggagctcaa ctgcgccgag ctcgacttca
     3181 agcgcaagct cgaaggcagc cacccgtcgc gtcgcgtcac catcggccgc tgcgccaatc
     3241 tcaccgagcc cacgcccgaa cacatcgcgc tcgggcgagg tccctgccag ttgcgcagcg
     3301 tgtgcgagcg aggatgcgga tacgggggat atttctcgac gctgagcgcc acgttgccgg
     3361 ccgcgcagcg taccgggaac ctgacggtcg tcaccgacgc cattgtcgag cgcctcgact
     3421 acgacaatgc caagcgccgc atctcggcgg ttcgcgtcat cgacgcgaaa acccgcgaac
     3481 atcgcagtta ccaggcccgc gtggttttcc tgtgtgcctc gaccatcccg accgcccagg
     3541 tcctgctcaa ctccagatcg gatttctttc cgaacggcct cgcgaaccgg tcggaccagg
     3601 tgggccggaa tctcatggac catgtgcacg gcatcggcgc catcggcaac gttcccggat
     3661 tcctcgatcg ctactactac ggccgccggc ccacgggtct ctacattccg cgctacgcga
     3721 acgtcaccga ggacggtcgc ggcgagtaca cacgcggctg ggctttccag ggcaagggcg
     3781 agcggccgaa ttgggaccgc gcactcgaca tgccgagcgt gggcgcggaa ctcaagaccg
     3841 agctgcgcga acccggtccc tggcagatgg tgctgttcgg ctttgccgag atgctcccgc
     3901 ggcccgacaa ccgcgtcacg ctgcatgaga gcaggaccga cgccgcggga atgccgctcg
     3961 tccacatcga ctgcacattt ggcgagaacg aacgcaagct cgcggccgcg gcggatcggg
     4021 acgcgctggc catgttgacc accgcgggtt gcaccaacgt gcgcgcgttg ttcccgcacc
     4081 tgtcgccgcc ggggaccagc gtccatgaga tgggaacggc ccgcatgggg cacgaccccg
     4141 cgacctccgt gctgaaccgg cacaaccagg cccacgacgt accgaacctg ttcatcaccg
     4201 atggctcttg catgacctcg tcgagctgcg tgaacccgtc gctgacctac atggcgctgt
     4261 cggcacgcgc cgcgcattac gcggcggacc ggctcgcctc aggagagctg tgacatggcc
     4321 taccggggtg ccgactgccg tggccggacg cgcacgacga gttcgcgaaa cccgggggtc
     4381 acctcgcgca accggggctc gatttcggcc agcgcgaacc gcatccgggc ggcccaggca
     4441 gaggattccg cgatgaccgt aatccttccg ctatccaggc tgcaccgatt taccttattc
     4501 gcgagctctt ctcccagcgc cgtcattact gcgacctgcc acacggacgg ttcggacctc
     4561 gaaagggcac gctgggcgag agctggcagg cgcgccgaga ttaagtctcc cgcagtcctg
     4621 ggtttggagc ccggacggac ggtttgcttg tttcggtcag gtcttttggg catagtttcg
     4681 cgcctgctgg gcctaatggt tttgcggaca tccaggttgc cagcgaggta caagtctttg
     4741 aactacaaag aacaggatcg taaaggtccg atttgttacc tgctcccgga gctggcgcga
     4801 tgaacatcat cttcttctcg cgccgcgaag gccgtgcccg tcacttcaat ctcgctcatc
     4861 cgttcgccgt ggccgctttt gccgccgtgc tcgtcgccat tctggcgacc gctttcagtc
     4921 tcggattcaa gatcggtgag agcaaggcgc ccccgcgtgg ggtcgcttcg tggaccgcga
     4981 ccctcgctga acagcaatcc gaaatcaagg agctcaaggc cgagcttcag cgccgcgtcg
     5041 acgcggtggc catgcgtatc ggccagatga acgcccacgt catccgcctc gacgcgctgg
     5101 gcaagcgcct cacgcagatg gcgaacatcg acaaccgcga attcgacttc gagagccagc
     5161 ccgccatcgg cggacccgaa tccgacatcg gcgcagccgc catgcaggcc cccgacctca
     5221 actcgttgct gaacggtctc gagcagaaga tctcctcgcg cgacgcccag ctcgccgcgc
     5281 tcgaaaacgc actgctgtcg aagaagctcg acgagcagat ccgcccggac ggccggccgg
     5341 tgcgcgcagg ccacatctct tcgtatttcg gcgagcgcca ggatccgttc aacggtcacc
     5401 aggcgttcca caagggcatc gatttcgcga gccccaaggg ttccgatgtc gtggcggtcg
     5461 ccgccggcgt cgtcaccttc gcgggcgaga aggccggcta cggcaacctc gtcgaggtca
     5521 gccatggcaa cgggctgacc acgcgttacg ggcacaacca gggtcttgcg gtcggcgtgg
     5581 gccgtaccgt ggcccgcggt gacaccctgg ccttcgt
//
LOCUS       DATDEU010000315         6214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_495159, whole genome shotgun sequence.
ACCESSION   DATDEU010000315 DATDEU010000000
VERSION     DATDEU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495159"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..906)
                     /locus_tag="VIV63_07500"
     CDS             complement(160..906)
                     /locus_tag="VIV63_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6124481.1"
                     /translation="MKTLLACALLSLAVPAFAAAATPIETAIANPERTAKDRERDALE
                     KPAEVLAFAGAKPGMTIADLFAGGGYYSELLAGVVGPNGKVLSVNNVPYSAYAKDAVA
                     ARFTEGRLKNVERRLVEASYMNLPPKSVDLIVICMSYHDAFWIDEKEGWPEISIDGFV
                     DSMKRMLKPGGKLLIIDHNAPAGTGREMAGKAHRLNEDFAKQSLTSRGFVLEKTYDGL
                     RNKSDQLDKLVFDPAVKGKTDRYVHLYKVK"
     gene            1016..1717
                     /locus_tag="VIV63_07505"
     CDS             1016..1717
                     /locus_tag="VIV63_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6124482.1"
                     /translation="MRNSFKPIVLTLLLATTCTAFGAGGGEMPNRSSGSASRLDSAKE
                     TPEEKARNAYNAGVRLVQKADELSVDAAHQADERKKAKGRDKAQQTYTSAMKKFTSAT
                     ELAPTDFQAWNYLGYTYRKLGQYDVALAAYDRSLKLQPNYANAIEYRGHAYLGLNRLD
                     DAKAAYLALFAGNRKLAATLLAAMQEWVSDRRSNAAGIDGNTLESFASWVSERNTIAS
                     QTASLTREGAAAAWH"
     gene            1740..2720
                     /locus_tag="VIV63_07510"
     CDS             1740..2720
                     /locus_tag="VIV63_07510"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY6124483.1"
                     /translation="MNLAKRIAVMAAVAAVAACAQAATGEFRWQLPAGFPEPAVPVEN
                     PMSDAKVALGEKLFADSRLSMTGQHSCASCHSPERAFTDGLARSRGAAGDSLPLNAPT
                     LLNVAYNASLGWRDANVRTLEQQMRGPLFNEHPHELGLAGRERHVERELLADDTMVRS
                     FGAAFPGEDQPVSIDNVIRAIAAYERTLFAGHSAFDRYVFEGDHRALSNRQKAGMELF
                     YSARTGCAGCHGGVNFAGTWVDRHHREAEPVFADTGTGVAVRVPTLRNLSATAPYLHD
                     GRLQSLDAVLDHYERLAQDPAADPRLRRAALTTAERESLREFLLCLTDTR"
     gene            2740..3312
                     /locus_tag="VIV63_07515"
     CDS             2740..3312
                     /locus_tag="VIV63_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEY6124484.1"
                     /translation="MNLGLTLGRHILLPHPVSEPGALQDLVHELSVSVVREQAGQLKL
                     IYRLAGDLDALQLPEPRAAVRTDGLWRHSCFEAFIGHAGGPDYREYNFSPSGAWAAYH
                     FSAYREGMAPLMKGSPPTVTPRIGSETVELTALLDLSWLPRSAADAGLRLGLAAVVED
                     RARVLSYWALKHPAEKPDFHHADSFVVELD"
     gene            3346..4554
                     /locus_tag="VIV63_07520"
     CDS             3346..4554
                     /locus_tag="VIV63_07520"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY6124485.1"
                     /translation="MIQFGIDRLLGDMKLLRELEGRRVALLAHPASMTAEFEHSLDAL
                     RGVRGLDITAAFGPQHGLRGDKQDNMIESPDFRDPVHGIPVFSLYGQVRRPTEDMMDH
                     FDVLLVDLQDVGCRIYTFITTLLYVLEAAAKHGKTVWVLDRPNPAGRPVEGLALREGW
                     ESFVGAGPIPMRHGLTLGELGMWFVSHFHLAVDYRVIPMRGWKPDEAPGFGWPIGERN
                     WVNPSPNAANLSMVRAYAGTVMLEGTTLSEGRGTTRPLEMLGAPGLDPRTLLTAMDRI
                     VPGWIRGCRLRPCWFEPTFQKHSGKLCSGFQIHVEDGAYAHGEFRPWRLIAGAFKCIR
                     RLRGEYPLWRDFPYEYEHSRLAIDLINGSELLRQWVDDPAATPADLEALAAADEAAWE
                     EQRSELLLYR"
     gene            complement(4574..4954)
                     /locus_tag="VIV63_07525"
     CDS             complement(4574..4954)
                     /locus_tag="VIV63_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEY6124486.1"
                     /translation="MTRLNSAFVLRRTSERLTAGAAAIGVTFLMLLTVDVRAAETIDN
                     PPRISVSYQDTEFATQKGTANVYRKLKMAARKVCGLAPGGALTLDQRHNAEKCVDGAL
                     ADAVQRINRAMLTSVHEASSRDLG"
     gene            complement(5187..6194)
                     /locus_tag="VIV63_07530"
     CDS             complement(5187..6194)
                     /locus_tag="VIV63_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY6124487.1"
                     /translation="MAAKKKAPSKGKTKGKRATKLAPNAIKRGLSAIEVALALDHADI
                     AAVVDLVNKAGGAPLGAYREPLGGRPLLLASLPFSAVQPTPFQRDRSPTHAKRLAGAI
                     EATAAFLDPLIVVRGEDGRFWTPNGRHRLAAAKMLGLKQITVLISPDETLSYRILALN
                     TEKAHNLKDKSLEVVRMARNIAKREPARTEASLAAEFEAAELLTLGLVYEQNLRFGGG
                     AYSPVLKKVDRLTTKTLPVGLRERTAYASRLQEIDTEVKRIVAELQARGMKSPYLRSY
                     VVARINPVRFHKVKAGDKKPVMPIAQALLRMVAAAKKFDLGKVNMSDLAFVAAGAESA
                     E"
BASE COUNT         1069 a         2024 c         1969 g         1152 t
ORIGIN      
        1 tgccggcgat ggctttctca tccttgcgca cgatggcctt gtcccagtca tccgacagcc
       61 gcgtgagcgt cgcgacgtcg ctcgcgtcgt cagccagggc ggcattcgcg ccgcctaacc
      121 acaacatcgc gaatacgagc aacgcgagcc gcgtcatgcc tacttcacct tgtacaggtg
      181 cacgtaacga tccgtcttgc ctttgacggc cggatcgaag accagcttgt cgagctgatc
      241 gctcttgttg cgcaggccgt cgtaggtctt ctcgagcacg aagcctcgcg acgtcaggct
      301 ctgcttcgcg aagtcttcgt tgagacggtg tgccttgccc gccatctcac gcccggttcc
      361 ggccggcgcg ttgtggtcga tgatcagcaa ttttccgccg ggcttcagca tgcgcttcat
      421 cgaatcgacg aagccgtcga tgctgatctc gggccagcct tccttctcgt cgatccagaa
      481 cgcgtcgtga taggacatgc agatcacgat gaggtcgacg ctcttcggcg gcaggttcat
      541 gtagctcgct tccaccaggc ggcgctcgac gttcttgagc cggccttcgg tgaagcgcgc
      601 cgcgaccgca tccttcgcat atgcggaata gggcacgttg ttgaccgaca gcaccttgcc
      661 gttggggccc accacacccg ctaacagctc ggagtaatag ccaccgcccg cgaagagatc
      721 ggcgatggtc atgccgggct tcgcgccggc gaaagccagg acctctgcgg gcttctcgag
      781 cgcatcgcgt tcgcgatcct tggcagtgcg ttcgggattg gcgatggcgg tttcgatcgg
      841 cgttgccgcc gcggcgaacg cgggtacggc cagtgagagc agggcgcagg cgagcagtgt
      901 tttcatgagg ttctccttgc gtgcattatg cgaaacccgg cgatgacatt ggaacctcgc
      961 ccatcgcgcg ggttactcat tctggcgacc gacaagtcaa tcttcgggag atcccatgcg
     1021 caattcattc aaaccgatcg ttctgaccct gttgctggcc acgacttgta ccgctttcgg
     1081 ggcgggcggt ggcgagatgc cgaatcgttc gagcggctcg gcctcgcggc tcgactcagc
     1141 caaggaaacg ccggaagaaa aggcgcgcaa cgcatacaac gcgggtgtgc gactcgtgca
     1201 gaaggcggac gaactctctg ttgacgcggc gcatcaggcc gatgagcgca agaaagccaa
     1261 gggacgtgac aaggcccagc agacgtacac gtcggcgatg aagaaattca ccagcgccac
     1321 cgagctggcc ccgacggatt tccaggcgtg gaactacctg ggctacacct atcgcaagct
     1381 gggtcagtac gacgtcgcgc ttgccgcgta cgaccggtcg ctcaaactcc agccaaacta
     1441 cgccaacgcc atcgagtatc gcggacacgc gtatctcggg ctgaaccgcc tcgacgacgc
     1501 gaaagcggca tacctcgctt tgtttgccgg caatcgcaag ctcgccgcca cactgcttgc
     1561 tgccatgcag gaatgggtga gtgaccgtcg cagcaatgcc gcgggaatcg acggcaacac
     1621 actcgaatcg tttgcgtcct gggtgagcga gcgcaacacc atcgcgagcc agaccgcgtc
     1681 gctgacacgc gagggcgctg ccgccgcctg gcattgatat ccactcgggc tcacgccgca
     1741 tgaatctcgc gaaacgtatc gcggtcatgg ccgccgtcgc agcggtggcg gcctgcgcgc
     1801 aggcggctac cggggagttc cgctggcaat tgcccgcggg ttttcccgaa cccgcggtgc
     1861 cggtcgaaaa tcccatgagc gatgcgaagg tggcactcgg cgagaagttg ttcgccgatt
     1921 cgcgcctgtc gatgaccgga caacattcct gtgcgagctg ccattcgccg gagcgggcct
     1981 ttaccgacgg cctcgcccgt tcgcgcgggg ctgccggcga ctcactgccg ctcaatgcgc
     2041 cgaccctgtt gaatgtcgcg tacaacgcat cgcttggctg gcgcgatgcg aacgtgcgaa
     2101 cactcgagca gcagatgcgt ggcccattgt tcaacgagca tccgcacgag ctcggactcg
     2161 cgggacgcga acgtcacgtc gagcgcgagt tgctggccga tgacaccatg gtgcgcagct
     2221 tcggcgctgc gtttccaggc gaggaccagc cggtcagcat cgacaacgtc atccgcgcca
     2281 ttgccgcata cgaacgtacg ctgttcgcgg gccactccgc gttcgaccgg tatgtgttcg
     2341 agggcgatca tcgtgcgctg agcaaccggc agaaagccgg catggagctg ttctattccg
     2401 cgcgcacggg atgcgccggc tgccacggcg gcgtgaattt cgcgggcacg tgggtagacc
     2461 ggcatcatcg tgaggccgaa cccgtgttcg cggacaccgg cacgggagtg gcagtgcgcg
     2521 tccccacgct gcgcaatctt tcggcgaccg cgccctacct gcacgacggg cgcctccaat
     2581 cgctcgatgc cgtcctcgat cattacgagc gcctcgccca ggatccggcc gccgacccgc
     2641 gcctccggcg cgcggcacta actaccgccg aacgcgaaag ccttcgggag ttcctcctct
     2701 gcctgacgga cacccggtag cgtatgcttg cacgccagca tgaaccttgg actcactctc
     2761 ggccgccaca tcctgctgcc gcatcccgtc tccgagccgg gcgcgctgca ggacctcgtg
     2821 catgagctgt ccgtatcagt cgtccgcgag caggccggcc agctgaaact catctatcgc
     2881 ctggcgggcg acctcgacgc actgcagcta ccggaacccc gtgccgcggt gcgcacggac
     2941 ggcctgtggc gtcacagctg cttcgaggcg ttcatcggac atgcgggcgg acccgactac
     3001 cgggaataca atttttcccc gtccggtgcc tgggccgcct atcacttcag cgcttatcgc
     3061 gaaggcatgg cgcccctgat gaagggatcg ccgcccacgg tcacgccccg catcggcagt
     3121 gagaccgtcg agctcactgc actgctcgat ctttcctggc ttccacgttc ggccgccgat
     3181 gcgggactgc gtctcggtct cgcggccgtc gtcgaagacc gggcgagagt gctgtcatac
     3241 tgggcgctca aacatccggc ggagaaaccc gatttccatc acgcggacag cttcgtggtc
     3301 gaactcgatt gaccgccgca ttgcgcattt cctgaagggc cattcgtgat ccagtttgga
     3361 atcgaccgcc tgttaggcga catgaaattg ctgcgcgagc tggaaggccg gcgcgtggcg
     3421 ctgctggccc acccggcctc gatgacggcg gaattcgaac attcgctcga tgcattgcgc
     3481 ggcgtccgcg gtctcgacat caccgcggct ttcggaccgc agcacggcct gcgtggcgac
     3541 aagcaggaca acatgatcga gtcgccggat ttccgcgatc cggtccacgg catcccggtg
     3601 ttcagtctct acggccaggt gcgccggccg accgaagaca tgatggacca cttcgacgtg
     3661 ctgctggtcg acctgcagga cgtcggctgc cgcatctata ccttcatcac cactcttcta
     3721 tatgtgctgg aagcggccgc caaacacggc aagaccgtgt gggtgctgga ccgccccaat
     3781 ccggcaggcc gtcccgtcga ggggctcgcg ctgcgtgaag gctgggagag tttcgtcggc
     3841 gccgggccta tcccgatgcg ccacggtctc acgctcggcg aactcggcat gtggttcgtg
     3901 agtcacttcc acctggccgt ggactatcgc gtcattccca tgcgcggctg gaaacctgac
     3961 gaggcgccgg gtttcggctg gccgatcggc gagcgcaact gggtcaatcc cagccccaac
     4021 gcggcgaacc tctcgatggt gcgcgcgtat gcgggcacgg tgatgctcga gggcaccacg
     4081 ttgtcggagg gtcgcggcac cacgcggccg ctggagatgt taggcgcgcc cgggctggat
     4141 ccacgcacgc tgctgacggc catggaccgc atcgtcccgg ggtggatccg cggctgccgt
     4201 ctgcggcctt gctggttcga accgactttt cagaaacact ccggcaagtt gtgctcgggg
     4261 ttccagatcc atgtcgaaga tggcgcgtac gcacacggcg agttccgtcc ctggcgcctg
     4321 atcgccggag cgttcaagtg catccggcgc ctgcgtggcg agtacccgct gtggcgagac
     4381 ttcccgtacg aatacgagca ttcacgtctc gccatcgacc tcatcaacgg cagcgaattg
     4441 ctgcgtcagt gggtggacga tccggccgcg accccggcgg atctcgaagc tctggccgcg
     4501 gccgacgaag cggcctggga agagcagcgt tcagagctgc tgctctaccg ctgagcgcgg
     4561 ccaccttccg caatcaaccc aggtcgcgcg acgatgcttc gtgcaccgac gtcaacatcg
     4621 cccggttgat gcgctgcacg gcgtccgcca gtgcaccatc gacgcacttt tcggcgttgt
     4681 gccgctggtc gagcgtcaat gcgccgcccg gggcgagacc gcagaccttg cgggccgcca
     4741 tcttgagctt gcggtacaca ttcgccgtgc ccttctgggt ggcgaactcg gtgtcctggt
     4801 agctcaccga gatacggggc gggttgtcga tcgtctccgc ggcgcgcacg tcgaccgtga
     4861 gcagcatgag gaacgtgacg ccgatcgcgg cggcacctgc ggtcaggcgc tcggaagtgc
     4921 gacgcagcac aaaggcggaa ttcaggcgag tcatggctaa tcctccggtt atatagagcg
     4981 gaaaacctgc gatgacgcgt gccgactcat cgcttgccgg cgtgagaggc gagttcgaaa
     5041 cgtgtcaatc agaacgctga tatagacgcg cgagaacgcg aaacggttgc aaagaaagtc
     5101 gggggattgc gcctaagcgt tgaagggcag cgccaggcgg aaagtgtccg gtgcggacac
     5161 cctggaaaag gtttgaatcg gccgaactac tcggccgatt cggcgcccgc ggccacgaac
     5221 gcgaggtcgc tcatgttcac cttgcccagg tcgaacttct tcgcggccgc caccatgcgc
     5281 agcagcgcct gcgcgatggg catgacgggt tttttgtcgc ccgctttcac cttgtggaaa
     5341 cgtaccgggt ttatacgcgc caccacgtag ctgcgcaggt agggcgactt catgccacgc
     5401 gcctgcagtt ccgcgacgat ccgcttcact tccgtgtcta tctcctgcag gcgcgaggcg
     5461 tacgccgtgc gttcccgcaa ccccaccggc agtgtcttgg tggtgagcct atctaccttc
     5521 ttgagcaccg ggctgtaggc gccaccaccg aaacgaaggt tctgctcata caccagcccg
     5581 agcgtcagca actccgcggc ttcgaactcg gccgcgagcg aggcttcggt gcgcgcaggt
     5641 tcgcgtttgg caatattgcg cgccatgcgc acgacttcca ggctcttgtc cttgaggttg
     5701 tgcgcttttt cagtgttcag cgcgaggatg cggtaggaca gcgtttcatc cggcgaaatg
     5761 agcaccgtga tctgcttgag acccagcatc ttggcggcgg ccagtcgatg gcggccgttc
     5821 ggagtccaga aacgaccgtc ttctccgcgc accacgatga gcgggtcgag gaatgcagcg
     5881 gtggcttcga tggcccccgc caatcgcttg gcatgggtcg gggaccggtc gcgctgaaac
     5941 ggagtcggtt gcaccgcgct gaaaggcagc gaggcgagca gcagcgggcg gcccccgagc
     6001 ggctcgcgat aggcgccgag cggcgcgcca ccggccttgt tcaccaggtc gaccaccgcg
     6061 gcaatgtcgg cgtgatcgag ggccagcgcc acttcgatcg ccgacaggcc tcgcttgatg
     6121 gcgttcgggg cgagcttcgt cgcgcgtttg cctttcgtct tacccttgga cggtgctttt
     6181 ttcttggcgg ccatcggtgt ccctcacctc gtct
//
LOCUS       DATDEU010000316         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1370027, whole genome shotgun sequence.
ACCESSION   DATDEU010000316 DATDEU010000000
VERSION     DATDEU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370027"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /gene="rseP"
                     /locus_tag="VIV63_07535"
     CDS             <1..720
                     /gene="rseP"
                     /locus_tag="VIV63_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY6124488.1"
                     /translation="QYWTPPFPAVLGTVEAGGPADQAGLKVDDEIKSVDGTAIRSFGE
                     FRDLVRARPGQEISVTVKRAGGEVTRRVNIASETDGGQTVGRLRIGPKDTGVPYPPEM
                     RTHTPVGPLSALSYSVAKAWQMTAAQAKFFVRMLTGDVSWKNTSGFIGIAKSVGEAAE
                     SGVFTFLMMMVLLSLSLGLLNLLPIPILDGGQVVFQLVEWVKGSPLSDRAYLVGQQAG
                     LLLLVLLMGVALFNDLSNLNF"
     gene            745..>2633
                     /gene="bamA"
                     /locus_tag="VIV63_07540"
     CDS             745..>2633
                     /gene="bamA"
                     /locus_tag="VIV63_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY6124489.1"
                     /translation="MTIRFATLCAASGIVLAAPLHAQEGPAFTVGDIRVEGLQRVTEG
                     TVYNYLPINIGDNLSAQRIREAVRALYDTGFFRDVELRRDGNTLIVVVNERPTIESFE
                     LKGNKDIKTEDLSKSLRNVGLAQGKTFDRSVLEDVKSFLQDQYGARGKYAARIDAQVE
                     EVAGNRVKIKIDIVEGKRAKIRQINIVGNETFKEKDILEAFELKTPNLLSFYKQDDRY
                     SRESLQGDLEKLRSFYMDRGYANFEVESTQVAIAPEKDDIFITVNVNEGEVHKVKEVK
                     LAGTFVVPEPLLRRYLLVQPGDTFNRKAITTTQELLQNRLGEDGFAFAKVDPVPTTIG
                     DPANKELSLTFFVDPGNRVYVRHIAFNGVEKINDEVLRREMRQLEGGWLSNAALDRSK
                     QRLERLPYIKKVESETKPVAGSTDLVDVTYEIEEGPSAQLGGGVGYSESQSFILSGNY
                     ADANFLGTGRRVSIDLNSGKYSKVYGVSMTEPYFTANGVGLTGNVTYRDQTQFVSASS
                     DFSSETIGAGFDIGYPIGENQGIRMGLQFQKSNLLTSSGGSARQAIEWVAMNGDSTRE
                     CIDYDPRVPTCDVELWGTEYNTVEFTTSWNYDSRNRSLFADRGMRHSFGLAYALPGVS
                     DVQYYV"
BASE COUNT          522 a          831 c          814 g          466 t
ORIGIN      
        1 caatactgga cgccgccttt tcccgcggtg ctcggtaccg tggaagcggg tggaccggcc
       61 gaccaggctg gcctcaaggt ggacgacgaa atcaagagcg tcgacggcac ggcgatacgc
      121 agcttcggcg agttccgcga cctcgtacgc gcccgcccgg gtcaggaaat ctccgtcacc
      181 gtgaagcgcg ccggcggcga ggtcacgcga cgcgtcaata tcgcgagcga aacggatggc
      241 ggacagacgg tcgggcgcct gcgcatcggc cccaaggaca cgggcgttcc ctatccaccg
      301 gaaatgcgca cgcacacgcc ggtcggtccg ctgagcgcgc tttcctattc cgtcgccaag
      361 gcctggcaga tgacggcggc gcaggccaag tttttcgtgc gcatgcttac cggcgatgtg
      421 tcgtggaaga acacgtccgg attcatcggc attgcgaagt cggtgggtga agccgccgaa
      481 tcgggcgtat tcacttttct gatgatgatg gttctgctgt cgctgtcgct gggcttactc
      541 aacctgctgc ccatcccgat cctcgacggt ggccaggtcg tgttccagct ggtcgagtgg
      601 gtgaagggaa gcccgctgtc cgaccgcgcc tatctggtgg gccagcaggc gggactgctg
      661 ctgctcgtgc tgctcatggg cgtggcgctg ttcaacgatc tgtcgaatct gaatttttaa
      721 gaacgatttc tccaggggac tgacttgacc atacggttcg cgactctttg tgccgccagc
      781 gggattgtgc tggcagctcc actgcacgcc caggagggcc cggcgttcac cgtcggcgac
      841 atccgcgtcg aaggcctgca gcgcgtcacc gaaggaacgg tctacaacta tctacccatc
      901 aacatcggcg acaacctgtc ggcgcagcgt atccgcgagg cggtgcgcgc gttgtacgac
      961 acgggtttct tccgggacgt ggaattgcgc cgcgacggca acacgctgat cgtcgtggtg
     1021 aacgagcgcc cgaccatcga gagtttcgag ctgaagggca acaaggacat caagaccgag
     1081 gacctcagca agtcgctgcg caacgtgggc ctggcacagg gcaagacctt cgaccgctca
     1141 gtgctcgagg acgtgaagtc gttcctgcag gaccagtacg gcgcgcgcgg caaatacgcg
     1201 gcgcgcatcg acgcgcaggt cgaggaagtc gccggcaatc gcgtcaagat caagatcgac
     1261 atcgtcgaag gcaaacgcgc caagatccgc cagatcaaca tcgtcggcaa cgagacgttc
     1321 aaggaaaagg acatcctcga ggcgttcgag ctcaagacgc ccaacctgct gtcgttctac
     1381 aagcaggacg accgctactc gcgcgaatcg ctgcagggcg atctcgaaaa actgcgctcg
     1441 ttctacatgg accgcggtta cgcgaacttc gaagtcgaat ccacccaggt cgccatcgcg
     1501 ccggagaagg acgacatctt catcacggtg aacgtcaacg aaggcgaggt tcacaaggtc
     1561 aaggaagtga agctggccgg cacgttcgtc gtgcccgagc cgctgcttcg gcgctacctg
     1621 ctggtccagc cgggcgatac cttcaaccgc aaggccatca ccacgacgca ggaactgttg
     1681 cagaaccgcc tgggcgaaga cggtttcgcg ttcgccaagg tcgacccggt ccccaccacc
     1741 atcggtgacc cggctaacaa ggagctgtcg ctcaccttct tcgtcgatcc gggcaatcgt
     1801 gtctacgtgc gccacatcgc gttcaacggc gtcgagaaga tcaacgacga agtgcttcgc
     1861 cgcgagatgc gccagctcga aggcggctgg ctgtcgaatg ccgcgctcga tcgctccaag
     1921 cagcgtctcg aacgtctgcc gtacatcaag aaggtcgaat ccgaaaccaa gcccgtggcc
     1981 ggttccaccg atctcgtcga cgtcacctac gaaatcgagg agggcccgtc cgcgcagttg
     2041 ggcggtggtg tcggctactc cgaatcgcag tcgttcatcc tcagcggcaa ctatgcggat
     2101 gcgaacttcc tcggcactgg ccgtcgcgtg tcgatcgacc tcaactcggg caagtacagc
     2161 aaggtgtacg gcgtctcgat gaccgagccg tacttcacgg ccaacggcgt gggcctgacc
     2221 ggcaacgtca cgtatcgcga tcagacgcag ttcgtttcgg catcgtccga cttctcgtcc
     2281 gagaccatcg gcgcgggttt cgacatcggt tacccgatcg gcgaaaacca gggcatccgc
     2341 atgggcctgc agttccagaa gtcaaacctg ctcaccagct cgggcggcag cgcgcgccag
     2401 gccatcgagt gggtggcgat gaacggcgac tcgactcgcg agtgcatcga ctacgacccg
     2461 cgcgttccga cgtgcgacgt cgagctgtgg ggaaccgaat acaacaccgt cgagttcacc
     2521 acctcgtgga actacgacag ccgcaatcgc tcgttgtttg ccgatcgcgg catgcggcat
     2581 tcgttcggcc tcgcctacgc actgccgggc gtcagcgacg tccagtacta cgt
//
LOCUS       DATDEU010000317         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_347891, whole genome shotgun sequence.
ACCESSION   DATDEU010000317 DATDEU010000000
VERSION     DATDEU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347891"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VIV63_07545"
     CDS             complement(<1..469)
                     /locus_tag="VIV63_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY6124490.1"
                     /translation="MSLGWSRRAPVDTLVGTEAVVDFTIPLAELPRVSHELVSADGEA
                     KGQVRFSRQVGQAVADLEVSAQPEVVCQRCMQPMRWPVTVKSRIALVSDYTAADRVPE
                     GMEVFLVEADSVSVRDLVDEEILLALPHVARHEEGSECADSAMQLPGQESEDEE"
     gene            537..1133
                     /locus_tag="VIV63_07550"
     CDS             537..1133
                     /locus_tag="VIV63_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY6124491.1"
                     /translation="MSPNAPTRPLILASTSRYRASLLQRFGLPFTTHDPAVDETERHG
                     ESPRARAFRLSEAKAEAVATQFPQAIVIGGDQVPATSTTILNKPGNAANCREQLKLLS
                     GSTAEFYTACTLRCLETGVKLSHVDTTAVKLRPLADSEIDRYIEREKPFDCAGGFKAE
                     ALGIVLFERIDTEDPTAIIGLPLIWLAGALRAVGFAVP"
     gene            complement(1130..>2024)
                     /locus_tag="VIV63_07555"
     CDS             complement(1130..>2024)
                     /locus_tag="VIV63_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY6124492.1"
                     /translation="FLARILKDVPKTHIFRVIRKGEVRVNGKRAKPDQRLLASDIVRV
                     PPVRTGAAAPPRRAPPAMVQGLTGAIIYEDPRLLVIDKPAGVAVHGGSGVSFGVIEAL
                     RAARPDETLELVHRIDRDTSGLLMISRKPAALRTLHALMRDGKVEKRYLALVKGKWEL
                     GRKRIDVPLRTDIRVGGERTVKAHETGKEAVSVFKPVQFFGKKATLVEVSLETGRTHQ
                     IRVHAAHAGYPLAGDEKYGEEDFNAMAKEWGLKRMFLHAHQLSFTWPDTGVEFNASAP
                     LPADLAAVLDALSAPKEKKSS"
BASE COUNT          333 a          709 c          615 g          367 t
ORIGIN      
        1 cctcttcgtc ttcggattcc tgtccgggca gctgcatggc gctgtccgca cattcgcttc
       61 cttcttcatg ccgcgccacg tgcggcagcg cgagcaagat ctcttcatcg acgaggtcgc
      121 gaacgctgac gctatcggcc tcgaccagaa aaacttccat cccctcgggc acccggtccg
      181 ccgcggtgta atcgctgacg agcgcgatgc gcgacttcac ggtcaccggc cagcgcatgg
      241 gttgcatgca gcgctggcaa actacttcgg gctgcgcgct gacctcgaga tccgcaacgg
      301 cctgcccgac ctggcgcgag aagcgaacct ggcctttcgc ttccccatcc gcgctcacca
      361 actcatgaga aacccggggc aactcggcaa gcgggatcgt gaaatcgacc accgcttcgg
      421 tgcctaccaa ggtgtcgaca ggagcgcgac gggaccagcc cagtgacatg gggcgcgtat
      481 gatagggacc gcatcccgcc aagtcaaaac aacattaact ctaagccatt gtttttatga
      541 gccccaatgc gccgactcgc ccgttgatcc tggcgtccac ctcccgatac cgcgcaagcc
      601 tgctgcagcg cttcggcctg cccttcacga cgcacgatcc ggccgtggac gaaaccgagc
      661 ggcatgggga atcgccccgt gcgcgggctt tccggctgtc tgaggccaag gccgaggcgg
      721 tggccaccca gttcccccag gccatcgtga tcggcggcga ccaggttccg gccaccagca
      781 ccaccatcct caacaagccc ggcaatgcgg cgaattgccg cgagcagctc aagctgctgt
      841 ccggctcgac ggccgagttc tacacggcct gcaccctgcg ctgcctggaa accggcgtga
      901 agctctcgca cgttgatacg acggcggtga agctccggcc gctggcggac tcggaaatcg
      961 accgctacat cgagcgcgag aagcccttcg actgcgccgg tggtttcaag gccgaggcgc
     1021 tggggatcgt gctgttcgag cgcatcgaca ccgaggatcc gacggccatc atcggcctgc
     1081 cgctcatctg gctggccggc gccctgcgcg ccgtcgggtt cgcggttccc taactagatt
     1141 tcttttcctt cggcgcgctc aacgcgtcca ggacggcggc aaggtccgcc ggcagtggcg
     1201 cgctcgcgtt gaactcgacg ccggtatccg gccacgtgaa gctcaactgg tgcgcgtgca
     1261 ggaacatgcg tttgagtccc cactccttcg ccatcgcgtt gaaatcttct tcgccatatt
     1321 tctcatcgcc cgcgagtgga tagccggcat gcgcggcgtg cacgcggatc tggtgcgtgc
     1381 gtccggtctc cagcgacacc tcgaccagcg tcgccttctt gccgaagaac tgcacgggct
     1441 tgaagacgct taccgcttcc ttgcccgttt cgtgggcttt caccgtgcgc tctccgccca
     1501 cgcggatatc cgtgcgcagc ggcacgtcga tgcgtttccg gcccagctcc catttgccct
     1561 tcacgagcgc gagatagcgc ttctccactt tcccgtcccg catcaacgca tgcagcgtgc
     1621 gcagcgccgc gggcttgcgt gaaatcatca gcaacccgct ggtgtcgcga tcgatgcggt
     1681 gaaccagctc cagcgtttcg tcgggccgcg cggcgcgcag cgcctcgatg acgccgaagc
     1741 tcacgccgct gccaccgtgc acggcgaccc cggcgggctt gtcgatgacc agcagtcgcg
     1801 ggtcctcata gatgatggcg cccgtgaggc cctgcaccat cgcgggcggc gcgcggcgcg
     1861 gcggtgcggc agcaccggtt cggaccggcg gcacgcgcac gatgtcgctc gccagcaacc
     1921 gctggtcggg tttcgcgcgc tttccattca cgcggacctc gcctttgcgg atcacacgga
     1981 agatatgagt cttgggcacg tccttgagga ttcgggccag aaag
//
LOCUS       DATDEU010000318         2509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_57503, whole genome shotgun sequence.
ACCESSION   DATDEU010000318 DATDEU010000000
VERSION     DATDEU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57503"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VIV63_07560"
     CDS             complement(<1..432)
                     /locus_tag="VIV63_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124493.1"
                     /translation="MIIQRPDGKTGELVLLFHGVGSCANDLVPLGATAANAMPQAMVV
                     SVNAPHPSSLGRGREWFSVLGVTEQSRRGRVAQATGAFVDAVAHWQAEAGATPARTTL
                     IGFSQGAIMSLEAARLGPVLAGRIVSIAGRFFSAVPQNIPTS"
     gene            complement(437..1261)
                     /locus_tag="VIV63_07565"
     CDS             complement(437..1261)
                     /locus_tag="VIV63_07565"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY6124494.1"
                     /translation="MEHLPSLFISHGAPTYALEPGLAGAQLNALGRVLPKPRTVLVVS
                     PHWMTREAQVTGGIRPRTIHDFGGFDPALYDMSYPADGHPALAERAVELLRAEGWAAS
                     VNHDRGLDHGAWVPLMHLYPNHDVPVIQASMPSRLDAQSAFKYGRALAQLGKGGVLVV
                     GSGSLTHNLFEFRQGSESQADYASAFTAWIREAVLAGDEDKLLAALENAPHAARAHPT
                     TEHFLPLLIALGAAPSALPATVVPGGIVHGVLSMESYVFGAHVQLNLEREPAVRHA"
     gene            complement(1295..1747)
                     /locus_tag="VIV63_07570"
     CDS             complement(1295..1747)
                     /locus_tag="VIV63_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017068661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6124495.1"
                     /translation="MTNAIVQRLLAPGSGWGPLALRIPVGLIFMAHGAQKLFGAFGGY
                     GLEGTGQWMGSIGLAPGYLMALLAGSAEFFGGLALVLGLLVRPAAAALAFAMLVAIFA
                     VHIDKGLFVANNGYEFGLALFAVAVSLVVSGAGRASLDAALVSRSTQR"
     gene            complement(1765..>2509)
                     /locus_tag="VIV63_07575"
     CDS             complement(1765..>2509)
                     /locus_tag="VIV63_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEY6124496.1"
                     /translation="DNQSINLQFISLRGTFKVQLWRRKRLSHIRIFKGLIMKITILGS
                     GNMATAFVKQLNAAGHQVRVTGRNQAKASELAGQHRGVRSVAPNAAAQDADAIIVATP
                     YEAAADALRSLGDVRGKVVIDITNPLTPDYMGLTIGHDTSAAEKIAQATPGAEVVKGF
                     NTLFAQVLAAGADFGDGRKATVFLASDSERAKQTAQALAESMGFETLDAGPLKNARYL
                     EPVAGLNIYFGYGAGLGTTVAPTWLRNAA"
BASE COUNT          442 a          821 c          825 g          421 t
ORIGIN      
        1 ggacgtgggt atgttctgcg ggacagcaga aaaaaacctg cccgcgatcg atacgatcct
       61 tccggcgagc actggcccca gccgggccgc ctccagcgac atgatcgcgc cctgcgaaaa
      121 gccgatcagc gtcgtgcgcg ccggcgtggc gcccgcctct gcctgccaat gggcgacggc
      181 gtcgacgaag gcacccgtcg cctgggccac gcgaccccgg cggctttgtt ccgtgacgcc
      241 caagacggag aaccactcgc gcccgcggcc cagtgacgaa gggtgcggcg cgttcacact
      301 caccaccatc gcctggggca tggcgttggc cgctgttgcg ccgagcggca cgagatcgtt
      361 ggcgcaagac ccgacgccgt ggaacagcag caccagttcg ccggtcttcc cgtctgggcg
      421 ttggatgatc atggagtcat gcatggcgaa ccgccggttc ccgctcgaga ttcagctgca
      481 cgtgtgcccc gaacacatat gactccatcg agaggacgcc atgcacgatc ccgcccggca
      541 caactgtcgc cggcagggcc gagggtgcgg cacccaacgc aatgagcagc ggcaggaaat
      601 gttccgtggt gggatgcgca cgcgccgcgt gcggggcgtt ttccagtgcc gcgagcagtt
      661 tgtcctcgtc acccgccaga actgcttcgc gtatccacgc cgtgaaggca ctcgcatagt
      721 cggcctgcga ttcgcttccc tggcggaact cgaacaggtt gtgcgtgagg ctgcccgagc
      781 ccaccacgag caccccccct ttcccgagct gtgccagggc gcgcccgtac ttgaatgccg
      841 actgcgcgtc gagccgggaa ggcatcgagg cctggatcac gggaacatcg tggttcgggt
      901 acaggtgcat cagcggaacc caggcgccgt ggtccagtcc gcgatcatgg ttgaccgagg
      961 ccgcccaacc ttcggcacgc agcaactcca ccgcgcgctc cgccaaagcg ggatggccgt
     1021 ccgcggggta gctcatgtcg tagagcgcgg gatcgaagcc gccgaagtcg tggatcgtgc
     1081 gcgggcggat gccacccgtg acctgcgctt cccgcgtcat ccagtgcggc gacactacga
     1141 gcacggtcct cggtttggga agcacccggc ccagggcgtt cagctgggca ccggcgaggc
     1201 cgggctccaa cgcataggtg ggtgcgccgt gggaaatgaa aagactcggc aggtgttcca
     1261 tgatgggact ctccttgaaa gcgattcaca tacgttagcg ctgggtgctc ctggacacga
     1321 gcgcggcgtc cagcgatgcg cggccggcgc cgctgaccac cagcgacacg gccacggcga
     1381 acagggccag gccgaactcg tagccgttgt tggcgacgaa caggcccttg tcgatgtgca
     1441 cggcgaagat cgccaccagc atggcaaagg ccagtgcggc ggcggccggg cggaccagca
     1501 ggcccaaaac cagcgccagg ccaccgaaga attcggcgct gccggccagc aaggccatca
     1561 ggtagccggg tgccagaccg atggatccca tccactggcc cgtgccttcg aggccgtagc
     1621 caccgaacgc gccgaacaat ttctgggcgc catgcgccat gaagatcagc cccaccggga
     1681 tgcgcagggc cagcgggccc caaccggagc cgggggctag caggcgttga acaatagcat
     1741 ttgtcatttc agactccttg atggtcaggc ggcattgcgc agccaagtgg gggcgacagt
     1801 cgtccccagg cccgcgccgt agccgaaata gatgttgagg ccggcaactg gctcgaggta
     1861 tctggcgttc ttcagcggcc ccgcatccaa ggtctcgaac cccatgctct cggccagtgc
     1921 ctgcgcggtc tgcttggccc gctcgctgtc actggccagg aacacggtgg ccttgcggcc
     1981 gtcgccgaaa tccgcgcccg ctgccagcac ttgggcgaac agggtgttga atcccttgac
     2041 cacttccgcg ccgggggtcg cctgcgcgat cttctcggct gcggaggtgt cgtggccgat
     2101 ggtcaggccc atgtagtcgg gtgtgagcgg gttggtgatg tcgatcacca ccttgccgcg
     2161 cacgtccccc agggaccgca gcgcatccgc agcagcttcg tagggcgtag cgacgatgat
     2221 cgcgtccgcg tcctgcgccg ctgcattcgg tgcgacggac cggacaccgc ggtgttggcc
     2281 tgccagttcg ctggccttgg cttggttgcg tcccgtcacg cggacttggt gacccgcggc
     2341 attcaactgc ttaacgaatg cggtggccat gttgcctgag cccaggatgg tgatcttcat
     2401 gatgagtcct ttgaaaattc ggatgtgcga gaggcgtttt cgccgccaca actgcacttt
     2461 aaatgtcccg cgcaaagaga taaactgcag attgatagat tgattgtct
//
LOCUS       DATDEU010000319         5875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_348643, whole genome shotgun sequence.
ACCESSION   DATDEU010000319 DATDEU010000000
VERSION     DATDEU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348643"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VIV63_07580"
     CDS             <1..237
                     /locus_tag="VIV63_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124497.1"
                     /translation="LLPKTRQIRGQEAHGWEFASAGGNIVLWATGEGLPLEMTMGGSA
                     QIQLDFIFDFDLPLDAQLFSTAIPAGYSRAEPED"
     gene            241..636
                     /locus_tag="VIV63_07585"
     CDS             241..636
                     /locus_tag="VIV63_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017217706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6124498.1"
                     /translation="MQTLGMVSLVVADYDDALAFYVGKLGFRLVEDSFVPEQNKRWVV
                     IEPPGADGQGAKLLLARGSNPEQVSRVGNQTGGRVFLFLYTDDFWRDFNMYKARGIEF
                     SREPSEAPYGTVAVFKDLYGTLWDLVQLK"
     gene            758..3517
                     /locus_tag="VIV63_07590"
     CDS             758..3517
                     /locus_tag="VIV63_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6124499.1"
                     /translation="MFKLDRDAVARFRGGLNKAFTLGNLGIAPRLALAFASVAILAAT
                     SNLLVENGVAIVESVTRPGVFSPALSQEIGAQGPAFEKADARVKASRLVLALGKFDKA
                     VLEHVEAGTPDSTARFARARTEVRRFANAFEERHVQITGAPPRAFLAGLKTHEKSAAA
                     LIQTAQDRRVALTSYSTTFSRMKERLDGSMDRAWKIMGRVVARQSLLKLTAELDEIQT
                     RFATRNASGTAGDGQARLMNAEQAFAATLQQNEAALRRSQGAEWLAALRSDVTQLQGA
                     RAALAQVEQRQAFLTQTFSRETEPLVEVLVRISDKELKRAEVLPPPAPATQAPESAMP
                     AAAPVPAPKSMMQTRIRPVVAWVTVIVLGLLAYICVMTTLSVVRPVRRLLRATTKIAA
                     GRDVAPVPDGGIRELDTLSQAFNAMAKQLAAARAANREAQQSLEGRVEERTRQLQELA
                     ERDPLTGLANRRQLFAALNASIARTASTTKLIGAFFLDIDNFKTLNDSMGHAFGDRVL
                     TAIAHRLESAAKSFGFAARLGGDEFMLVHEGAESIDEIESSGNKIVDAFHHPIQIDGR
                     ELIVSVSVGASVYPLHAQDAEALLRAADAALFDAKAAGRSRLTIFTPELLVRASVKFR
                     TEQRLRRAIEKGEFELFYQPEVSVKNLEVSLVEALIRWRMPDGSYQCPADFLAVAEES
                     GLILEINDWVMRTAIETASQWHIGPWPEARVAINVSPRQFLDFRFVEKLQRLLEEFQL
                     PPRCLELELTESVLQTGPATIATLERLRAMGVAIALDDFGTGYSSIASLEQLPLTRIK
                     LDRSLIARMDSNPRSASIARATIGLCSELGLEVTAEGVERLEQFTALLGYESMSLQGF
                     LIARPVPRDQLLASVKGAAAHCQDLMLMSRKAEPGANEAVFTDAESRAVPAMGPTRLH
                     PPRRP"
     gene            complement(3521..4420)
                     /locus_tag="VIV63_07595"
     CDS             complement(3521..4420)
                     /locus_tag="VIV63_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124500.1"
                     /translation="MKSTFFAGLALALSLLAPAFSAPAKFEVGALQVEQTGKSEPAVI
                     LIPGLACGPWVWRDTAARLAKDHAVYLLTLPGFDGRAPQAGVTLETLQGDLLALIESK
                     HIRKPVIAGHSLGGTLALGFATRHSSSIAGVIAVDGLPVFPGTERMTGDRRPLGEQMR
                     AQIGGQTREQFVLGQQAYMKQIGSIDEAVAKMLAEQSSRSDTGATADFGAQMMMVDLR
                     AQLPQIKVPVVEISPFNAPDFAAMNVDEAGKTAYYRALLTGVGKLEVVSISPARHFVM
                     FDQPAKFAEALDHALASMAEPLR"
     gene            complement(4423..4617)
                     /locus_tag="VIV63_07600"
     CDS             complement(4423..4617)
                     /locus_tag="VIV63_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018766546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6124501.1"
                     /translation="MKNSVRELRTERGWSQGDLADKLEVSRQTVNAIETGRYDPSLPL
                     AFAIAKLFGKPIEKIFSTEK"
     gene            complement(4614..5000)
                     /locus_tag="VIV63_07605"
     CDS             complement(4614..5000)
                     /locus_tag="VIV63_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124502.1"
                     /translation="MKEQEANRKYRMEIGASMLLYLGVLTGSLFLAKPMEEGAARTLL
                     LLLPALPVFLAVWAIARHMRRMDEFLRLRSLEGLAIAAAVTAGLSLTYGFLESAGFPK
                     LSMFWVWGVMGFVWGAVACLRSLFSR"
     gene            complement(5108..5566)
                     /locus_tag="VIV63_07610"
     CDS             complement(5108..5566)
                     /locus_tag="VIV63_07610"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6124503.1"
                     /translation="MKRAPVATGGCQCGAVRYAFYSPLENAHVCHCRMCQRATGGVFA
                     ALAGTAPENFAWTRGTPSFFASSNLAKRAFCDKCGTPLSFKYDTANARVYATIGSLDH
                     PEQLTLVKQFGIESQLPWVKFCEDVPAERTGESAAAQEFLATLQDNQRAD"
     gene            complement(5563..>5875)
                     /locus_tag="VIV63_07615"
     CDS             complement(5563..>5875)
                     /locus_tag="VIV63_07615"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY6124504.1"
                     /translation="VRAAQQRSSESDANAFQVKMELQADCYAGIWAHHADRSRQLVEA
                     GDIDEALGAASAVGDDTIQKRVQGHVNQESFTHGSGAQRQQWFRKGLGSGLVQDCDTF
                     Q"
BASE COUNT         1058 a         1883 c         1887 g         1047 t
ORIGIN      
        1 ctcctgccga agacccggca gattcggggc caggaggcgc atggatggga atttgcttcg
       61 gctggcggaa acatcgtgtt gtgggcaacc ggtgaagggc tgccactcga aatgacgatg
      121 ggtggaagcg cgcagataca gctcgacttc atcttcgatt tcgacctgcc gctcgacgcg
      181 caattgttca gcaccgccat tcccgcgggc tatagtcgcg ccgaaccgga ggactgagag
      241 atgcagacat tgggaatggt gtcattggtg gtcgcggact acgacgacgc gctggccttc
      301 tatgtcggca agctcggctt caggctggtg gaagacagtt tcgtgcccga gcagaacaaa
      361 cgctgggtgg tcatcgagcc acccggcgcc gatggtcagg gcgccaaact actgctggcg
      421 cgtggctcga acccggagca ggtctcgcgc gtgggcaacc agaccggcgg gcgcgtgttc
      481 ctcttcttat ataccgacga cttctggcgc gacttcaata tgtacaaagc caggggtatc
      541 gaattttcgc gcgagccgtc cgaggcgccg tacggcacgg tcgcggtatt caaggacctg
      601 tacggcacgc tctgggacct ggtgcagctt aagtagtagg ggccggaatc cgccggattc
      661 cgcttacaac aaagcgtgaa ctgtgcacgt agttggcgga caattcgccc cgttggcgtg
      721 accgccgaac tacttttgac ggcaaactag cgcaccgatg ttcaagttgg accgagatgc
      781 tgtggcgcgc ttccggggtg gcctgaataa ggcgttcacc ctgggcaatc ttggcatcgc
      841 cccgcgtctg gcactggcat tcgcgagcgt cgcgatcctg gccgcgactt ccaacctcct
      901 cgtcgaaaac ggcgtggcca tcgtcgagtc ggtgacgcgc cccggcgtgt tctcgcccgc
      961 gctttcgcag gaaattggcg cgcagggccc ggcattcgag aaggcagatg cgcgcgtcaa
     1021 agcctcgcgc ctggtcctgg cgctgggaaa gttcgacaag gcggtactcg aacacgtcga
     1081 agcaggcacg ccggattcga cggcgcgttt cgcacgtgca cgaaccgaag tcaggcgttt
     1141 tgcgaatgct ttcgaagagc ggcacgttca aatcaccggc gccccgccgc gggccttcct
     1201 ggccggattg aaaacgcacg agaagagcgc ggcggcattg atccagaccg cgcaggaccg
     1261 ccgcgtcgcg ctcaccagtt actccacaac cttttcgaga atgaaagagc gactcgatgg
     1321 gtccatggac cgggcgtgga agatcatggg ccgcgtggtc gcccggcaat cactgctgaa
     1381 gctgaccgcc gagctcgacg aaatccagac tcgcttcgct acgcgtaacg cttcaggcac
     1441 cgccggcgac ggccaggccc ggctcatgaa cgcggagcag gccttcgccg ccacgctgca
     1501 gcagaacgaa gccgcgttgc gccgctcgca gggcgcggaa tggttagccg ccttgcgcag
     1561 cgacgtcacg caactgcagg gcgcgcgcgc ggcactcgca caggtcgaac agcgccaggc
     1621 atttctcacg cagacgtttt cccgggagac ggagccgctg gtcgaggtgc tcgtcaggat
     1681 ttcggacaag gaattgaaac gcgcggaggt gctgccgcca cctgcgcccg cgacacaagc
     1741 gccggaatcc gcgatgcccg ccgccgcgcc agtgccggcg ccgaaatcga tgatgcagac
     1801 gcgcattcgc ccggtcgtcg cgtgggtcac cgtcatcgtg ctcggcttgc ttgcgtacat
     1861 ctgcgtcatg accacgctca gcgtcgtgcg gcccgtgcgg cgtctgctca gggcgaccac
     1921 gaagatcgcg gcgggccgtg acgtggcgcc ggtgcccgac ggcggaattc gcgagctcga
     1981 cacgttgagc caggcattca acgccatggc gaagcagctc gccgcggcgc gcgcggcgaa
     2041 ccgcgaggcc cagcagtctc tggaaggcag agtcgaagaa cgaacgcgcc agcttcagga
     2101 actcgccgag cgcgatccgc tgaccggact cgccaatcgc cggcagttgt tcgcggcgct
     2161 caacgcgtcc atcgcacgca cggcttcgac gaccaaactc atcggcgcgt tcttcctcga
     2221 catcgacaat ttcaagacgt tgaacgacag catggggcac gcgtttggcg accgcgtttt
     2281 gaccgcgatc gcgcaccggc tcgaatccgc cgcgaaatct ttcggattcg cggcacggct
     2341 cggcggcgac gaattcatgc tggtccatga gggcgcggag tcgatcgacg aaatcgaatc
     2401 gtccggcaac aagatcgtcg atgcgttcca tcacccgatc cagatcgatg gcagggaatt
     2461 gatcgtcagc gtgagcgtcg gcgccagcgt ctatccactt cacgcccagg acgccgaggc
     2521 actgctgcgc gccgcggacg cggcgctgtt cgatgcgaag gcggcgggac gcagccggct
     2581 cacgatcttc acgccggaat tgctggttcg cgcctcggtg aaattccgca ccgagcagag
     2641 attgcggcgc gccatcgaga agggcgaatt cgagctcttc tatcagccgg aggtcagcgt
     2701 caagaacctc gaggtatccc tcgtcgaagc actgattcgc tggcgcatgc cggatggaag
     2761 ctaccagtgc cccgcggatt ttctcgcggt ggccgaagag tccggcctca ttctcgagat
     2821 caacgactgg gtgatgcgta ccgccatcga aaccgcatcg cagtggcaca tcggtccgtg
     2881 gcccgaggcg cgcgtggcca tcaacgtatc gccgcgccag ttcctcgact tccggttcgt
     2941 cgaaaagctg cagcggctgc tggaggaatt ccagttaccg ccccgctgcc tcgagctcga
     3001 actgacggaa tccgtattgc agaccggccc cgcgaccatc gcgacgctcg aacgcctgcg
     3061 cgcgatgggt gttgcgattg cgctcgacga cttcggcacg ggctattcat ccatcgcctc
     3121 actcgagcag ttgccactga cgcgcatcaa gctcgaccgc agcctgatcg cacgcatgga
     3181 ctccaatccc cgttccgcat cgatcgcgcg cgcaaccatt ggtctgtgca gcgagctcgg
     3241 actcgaggtg accgccgaag gcgtcgagcg gctcgaacaa ttcacggcgt tgctgggtta
     3301 tgagtccatg tcgctgcagg gcttcctgat cgcccggccg gttccgcgcg accagctgct
     3361 ggcttcggtg aaaggcgcgg ccgctcattg ccaggacctc atgttgatgt cgcgcaaggc
     3421 tgagccgggc gcgaacgaag cggtgtttac cgacgctgag tctcgggccg ttcccgcaat
     3481 gggcccgacg cggctgcatc cgccgcgccg gccctgagct ctaccggagt ggctcggcca
     3541 tcgatgccag ggcgtggtcg agcgcctcgg cgaacttcgc gggctgatcg aacatcacga
     3601 agtgacgtgc cggcgagatg gaaacgacct ccagctttcc aacgcccgtg agcagcgcgc
     3661 ggtagtaggc cgtcttccca gcctcgtcga cattcatcgc cgcgaaatcc ggcgcattga
     3721 agggcgagat ttccacgacc ggcaccttga tctgcggtag ctgcgcgcga aggtcgacca
     3781 tcatcatctg cgcgccgaaa tcggcggttg cgcctgtgtc gctgcggctc gattgctccg
     3841 cgagcatttt cgcgacggcc tcgtcgatgc ttccgatctg tttcatgtac gcctgctgtc
     3901 cgagcacgaa ttgctcgcga gtctgcccgc cgatctgcgc gcgcatctgc tcgccgagcg
     3961 gtcgccgatc gcccgtcatc cgctcggtgc ccggaaatac cggcagcccg tcgaccgcga
     4021 tcacgccggc aatggacgag gaatgccggg tcgcaaaacc cagggccaga gtgcctccca
     4081 ggctgtgtcc ggcgatcacc ggcttgcgaa tgtgcttcga ttcgatgagc gcgagcaggt
     4141 cgccttgcag ggtttcgagc gtgacgccag cctgaggcgc acgtccatcg aagccgggca
     4201 acgtcagtag atagaccgcg tgatccttgg ccagccgcgc ggccgtatca cgccacaccc
     4261 acggcccgca ggcgagtccc gggatcagga tgacggcggg ttcgctcttg ccggtctgct
     4321 cgacctgcag tgcgcccact tcgaatttcg ccggcgcgga aaacgcgggc gcgagcaggg
     4381 agagcgcgag cgcgaggccc gcgaagaatg tcgacttcat ggttatttct ccgtggaaaa
     4441 gatcttttct atcggcttgc cgaaaagttt cgcgatggcg aacgccagcg gcaggctggg
     4501 gtcgtagcgg cccgtctcga tggcgttgac ggtctggcgc gacacctcca gtttgtcggc
     4561 gagatcgccc tggctccagc cgcgttccgt gcgcaattcg cgcaccgagt ttttcatcgc
     4621 gagaacaggc tgcgcaggca ggcgactgcg ccccagacga agcccatcac gccccatacc
     4681 cagaacatgc tcaacttcgg gaatcccgcg gactcgagaa agccgtaggt gaggctcagt
     4741 cctgcggtca cggccgcggc aatcgcgagg ccttcgaggc tgcgcaaacg caggaattcg
     4801 tccatgcgcc gcatgtgacg cgcgatcgcc cacaccgcga ggaataccgg caacgccggc
     4861 aggagcagca gcagagtgcg tgcggctcct tcctccatgg gtttggcgag gaacaacgag
     4921 cccgtcagta cgccgaggta cagcagcatc gaagccccga tttccatgcg gtatttccga
     4981 ttggcttcct gctctttcat cgtcgtctcc tgcctgggtc tgtaaaggat gcttgacagg
     5041 ctagacgtcg cgaaacgcct tgtcaagctt cctttacata gggagatcag gcggcggccg
     5101 ccggaagtta gtcggcgcgc tggttgtcct gaagcgtcgc caggaattcc tgcgccgcgg
     5161 cggattcgcc cgtgcgctcc gccggcacgt cctcacagaa tttcacccag ggcaattggc
     5221 tttcgatgcc gaattgcttc accagcgtga gttgctcggg atgatcgagc gagccgatgg
     5281 tcgcgtacac gcgagcgttc gccgtgtcgt acttgaagga caaaggcgtg ccgcatttgt
     5341 cgcagaacgc gcgcttggcg agattggagc tcgcgaaaaa cgacggtgtg ccgcgtgtcc
     5401 acgcgaagtt ttccggagcg gttcccgcga gcgccgcgaa gacgccgccg gtcgcgcgct
     5461 ggcacatgcg gcagtgacac acgtgtgcat tctcgagagg ggaataaaac gcgtagcgca
     5521 cggcgccgca ctgacatccg cccgtggcca ccggcgcccg cttcactgga acgtgtcgca
     5581 gtcctgcacg agccccgaac ccaggccctt gcggaaccac tgctggcgct gagcgccgga
     5641 accgtgcgtg aacgactcct ggttcacgtg gccctgcacg cgtttctgaa tggtgtcgtc
     5701 gccgacggcc gatgcggcgc cgagcgcttc gtcgatgtca ccggcttcga cgagctggcg
     5761 tgaccggtcg gcgtggtgtg cccagatgcc ggcgtagcag tccgcctgca gttccatctt
     5821 cacctggaac gcgttggcgt cgctttcaga tgagcgttgc tgggccgcac ggact
//
LOCUS       DATDEU010000320        13316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1808723, whole genome shotgun sequence.
ACCESSION   DATDEU010000320 DATDEU010000000
VERSION     DATDEU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808723"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2511
                     /locus_tag="VIV63_07620"
     CDS             <1..2511
                     /locus_tag="VIV63_07620"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY6124505.1"
                     /translation="YVAKVGTGFTLLFIGAIVFMAYKRLPLIAFTVVFTLLLAGYTYI
                     APPAGIWKGFLWVLLAALWLFNLRPLRKAIISRPFLKAYMRLLPSMSQTEKEALEAGT
                     VWWDGELFTGKPKFEKLLASKAPQLNAEEQAFIDGPCEELCRMYDDWEVTHKLGDMPT
                     HVWDYLKHKGFFAMIIPKKYGGLEFSAYAHSCVLVKLASRSGLLAATTAVPNSLGPAE
                     LLNHYGTEEQKNHYLPRLARGEEVPCFALTHPRAGSDAASLPDTGVVCKGLYQGKEIV
                     GLKLNFSKRYITLAPIATVVGLAFRLYDPDKLLGGDKSDYGITCALIPRATPGLIIGR
                     RHFPLNVPFQNGPLSGKDVFVPLDTIIGGPKMAGQGWRMLVEQLSVGRCISLPSNATG
                     GAKAGVFASGAYTRIRRQFNMPVGKFEGVEAVIARMVGYTYTMDAARSVTAGAIDGGE
                     KPSVPSAMLKYHVTEMGRQVSNDAMDVHGGKGIQLGPRNYLGRSYQVVPVAITVEGAN
                     ILTRSLIIFGQGAIRCHPFVLKEMNAARNPDRKQGVDQFDTALFGHIGFTISNAVRSF
                     VMALTHARFTHVPDVGDTRRYYQHIVRFSASFAFAVDVAMLTLGGYLKKKENLSARLG
                     DVLSAMYLASMVLKHHENQGRPADDLPVVEWACRNQLYKAQDQLHDFLRNFPNRFLGW
                     IMRALIFPGGRAYSAPSDRLGRTLADAVLNPSEVRDRLCRFVYRTLEPGNPLGLLQEA
                     LVMSQTAEPIEKRIRVDGVKTGKVTALDVPGQITQALSEGIISDTEAAMLRDYDRKVM
                     ELISVDDFESHELAAQAVQPDDKTSGSVSDLFPSGQVA"
     gene            2521..3543
                     /locus_tag="VIV63_07625"
     CDS             2521..3543
                     /locus_tag="VIV63_07625"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEY6124506.1"
                     /translation="MSSPPVPVVSPPETPQPLTLVCANCHAPLNGEYCSACGQRHEPH
                     IHTVVHFAGEAFESVSHADSRLWRTIGYLLTRPGYLTREFFAGRRVAYLPPFRLYLVI
                     SVLFFLVAGVPKDAEIVRIDAEATSETAGADQKPKVAFSIDAPKDAAGELNAAIAEAK
                     KAAAEGQPGVDVSDGVRDFCDQFKDQPTSDNEARNNLRDRCRAIASDGGRELAASIVH
                     NVPRAMFIFLPIIAFIMKLMYWRPKRYYVEHLLFLIHNHAFVFLVLIPLVLIGRIPYV
                     GDYSGWLWFAAIVYMIWYTYRGMRNYYSQGRALTLTKYFTLGFSYLITTFVVFLLVAI
                     YSAMMA"
     gene            3573..4004
                     /locus_tag="VIV63_07630"
     CDS             3573..4004
                     /locus_tag="VIV63_07630"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEY6124507.1"
                     /translation="MNNRKLVSFLCGSALAFGSLLALAREPSPAGAEVYIVSPKDGAK
                     VKGPVTVVFGLKGMGIAPAGIKFDNTGHHHLLLDADAPADLSLPLPADPHNIHFGKGQ
                     TETTLTLAPGTHTLQLLLADSLHTPHDPAVISKKITITVEK"
     gene            4019..4942
                     /locus_tag="VIV63_07635"
     CDS             4019..4942
                     /locus_tag="VIV63_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY6124508.1"
                     /translation="MNWRIHCAAAALATLAAATAAAQQVPDREYRPAIGKPEYASGQG
                     PVVCVDEAHHNFHTLAERFWAFGSLLKQDGYQVVPSVSAFDASSLARCAVLVISNAQP
                     SDAEWDQYPYPTPSAFTDAEIAAVHRWVGKGGALLLIADHMPLAGAAAKLAAAFDVEF
                     MDGFAVRDPQSREPDLFRAANQTMRDHVIVRGRNAPESVASIRTFTGQAFRAPKAEPL
                     LVIPAGYVGLMPKKAWEFTADTRRVAIEGWLQGAVQEVGKGRAAFFGEAAMFSAQLAG
                     PDKQPMGMNAPGAESNFRFTLNLLHWLTRTL"
     gene            4952..6226
                     /gene="dinB"
                     /locus_tag="VIV63_07640"
     CDS             4952..6226
                     /gene="dinB"
                     /locus_tag="VIV63_07640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY6124509.1"
                     /translation="MSAPRAILHVDMDAFYASVEQRDRPELRGQPLIVGGTSNRGVVA
                     AASYEVRKFGVRSAMPIREALQRCPHAICVKPRMSVYREVSHQVFAVFHEYTPIVEGL
                     SLDEAFLDVTASLSLKGDAITIARGIKQRIREETQLAASVGVAPNKLVAKIASELEKP
                     DGLTVIHGGNLRAVLDPLSVRRLPGLGRKLGERVEAAGLATFGQLRTAPDTVLWPIFG
                     RDSQRMRERASGIDERPVMSEWDEKSISAEETFFTDLADHSRMHSEVLRLADRAGTRM
                     RAQNLATGCVAVKIRRADFTTFTRQRRFEPSTTDSRTIAKIAAELLTAWLAEQPAARV
                     RLLGVGVNHLHAADQMDLFAAPAAPGAANTGATALDATVDLIRERFGNLALRRGSALP
                     EARGEEEPAEDMSEKAPGRGKPRGHAPTGGRG"
     gene            6314..7915
                     /locus_tag="VIV63_07645"
     CDS             6314..7915
                     /locus_tag="VIV63_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6124510.1"
                     /translation="MYSSFFGLNEKPFSITPDPRYLFLSERHAEALAHLVYGVNEAGG
                     FIQLTGEVGTGKTTVVRSLLAQAPKHAEIALILNPRMTPAEFLLAICEELRLTVAAGS
                     EHSIKDLVDLLSRHLLKAHAEGKRIVLVVDEAQNLSVEVLEQVRLLTNLETETQKLLQ
                     IILIGQPELRELLGRVELRQLAQRITGRYHLDPLSGPETAAYVRHRLRVAGSTREIFS
                     NGALREIQRLSGGVPRLINIISDRALLGAFTEDRHLVNAGVVRRAAGEVLGRSFQPRW
                     LPWTAGAGVAAIAIASAVLMLPRLLTTQTPAPVAAAAPAPAPAPVAPPPNLDQLLGQY
                     PLETDPDNAFNKLFALWGLRYVAGSVDPCSQALQAGLECLTQRGSLAQLRLFNRPAVL
                     NVIDSAGHAHQLVLAGLDDEHAQVDLGGAQREVGIGDLSRSWFGDYVLLWRPATGGSQ
                     PLALGARNARVRWLRDSLRRVNGLPADTVPNDRFDAELATLVEDFQRKNRLAVDGIAG
                     VQTQVALDAALNDAATPFLRNSGGS"
     gene            7917..8627
                     /locus_tag="VIV63_07650"
     CDS             7917..8627
                     /locus_tag="VIV63_07650"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HEY6124511.1"
                     /translation="MSFILDALKKSENDRQRQSAPALFEVKVAAPRRRFPLWAAGLAI
                     LLGVNILALLWFVMREPAAPVAPASAAIAPAPAPAAAPPGMVTVPATSTYIPANAPPA
                     VTVGTDTGIVAPSTAPPLAEEPMLSGQEPSVPPDYDARDYRPAITPDQAAAVAAARRE
                     GAAPSRDEVLAEGTQLPDLRLDLHVYDPDPSKKFVFINMRKLREGESLPEGVQVISIT
                     QTGARLTYRGTQFTLEAN"
     gene            complement(8624..9019)
                     /locus_tag="VIV63_07655"
     CDS             complement(8624..9019)
                     /locus_tag="VIV63_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6124512.1"
                     /translation="MNRLVTLLVGALLFAATPAFADDSNAAPITQVELLKRLEQKDPN
                     LVVLDVRTADEFAAGHVPGARNVSHDQLASRLDELAGARDKQVVLYCRSGRRTLLAED
                     ILRKAGFKNLAHLEGDYLAWEAEKRPVER"
     gene            complement(9036..9524)
                     /locus_tag="VIV63_07660"
     CDS             complement(9036..9524)
                     /locus_tag="VIV63_07660"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6124513.1"
                     /translation="MERMLNIRPATKADAPTIANLVRELADYEKLLHEAKATPEDFLR
                     EIESPNPVIHVLIAEWDGAPAGFALYFFNFSTFVGRPGLYLEDLFVRPEQRSHGIGRA
                     LLRALARIAEERGCGRMEWAVLDWNEPALRFYKSLDARPMKEWIVHRLTPVEIARLAA
                     EA"
     gene            complement(9524..10324)
                     /locus_tag="VIV63_07665"
     CDS             complement(9524..10324)
                     /locus_tag="VIV63_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124514.1"
                     /translation="MSVAQELHPDEVEKLRTRLLTLFRAEASRSDNTYRSRLFGPGKS
                     LAELERMTPVAFYAALSDRVRFRARLYEKVDWLGTVADGKVVYVIGKGIQPKERGAVK
                     VLVTVALAQYGAQWRAIVPTEVEAQLDDLIEGRVAAAPPPTGGAPGRSLVEERPQPLA
                     PGISELLARAEASLAEGHCEEYYSQFMSPNFNRATSKSARKTLVTACTNSESTRETMI
                     TTLRVVQELSPKYDTGGARAIYDVSGQGLPYERFVLERDKDMRWYIAE"
     gene            10682..12826
                     /locus_tag="VIV63_07670"
     CDS             10682..12826
                     /locus_tag="VIV63_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6124515.1"
                     /translation="MLPQSEFESPMLANAAAPVAIAAITQDRALIGLLRSVIDPANDL
                     ILVTSEAELTPHLNSRRVSVALLDSMFIEGDLASMAERLRETWPDLVLVVVGTAEEQS
                     KVAGQITSGVVYRFLHRPVSAPRVRLFVDAALRRHEVENVERTLEQTRPDFTRFEKAA
                     KKTGAPSPIRAVLPGIIGAVVLIAAAGFWFARPGSRQEAAPEAVAQVDSPPADSAPAD
                     TASAEPVAQPAPPPRESEPQALAPEPVSAPVVAQAAPDESVSERLRPATSQPAVVQAP
                     EELAPRVPPQPVLSFEQRLAEQLAHAESALQRGELASPPGRNAVELFRGALDLDPTNA
                     LAKGGLVRVADRLLSAAERAITAGSVEDAKKMVAVAESLTPATARGAFLMMQIEREQE
                     RAALTQAKNSDAQDKLAKGATYLRLAESRLASGALIEPSGDNARFYFEAARQLIPDDP
                     GLRETERALQKQLLDRASTAADAGNAAETERWLANADGAGAPRAEMASIRRSMHDTLN
                     GARASRVTTLTRSFTNALAANRLLQPADDNAKAHLLAMTNTDAGNPAVATARQSLGSA
                     LLGQGRDALTRGDLTAADTWLNEARSIGFSSDELKNAEAEIATAREKATQRTAATAVV
                     GEKSLERLEYVAPKFPAATRNRGVSGWVELEFTVLADGSTSDVVVTNSNPRKTFDASA
                     VTAVSQWRYKPVVRSNKVVEQRVAVRIRFSDQ"
     gene            12827..>13316
                     /locus_tag="VIV63_07675"
     CDS             12827..>13316
                     /locus_tag="VIV63_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124516.1"
                     /translation="MDAPRIQRFLIVETDTRYAQWLQHTVEAGWSNDSIVIMDWSSFA
                     RVRTAMTVRDYDIVMLSMVFDEGIEDPTTDSFDWIRKLRSQPNFPEMIILADGGSELA
                     AIRTLRLGAADYLPKRLLTPPRLQRSIKLTLRAIEKEALRRAERNAQPAAKTETDKLA
                     ESL"
BASE COUNT         2299 a         4589 c         4171 g         2257 t
ORIGIN      
        1 tacgtggcca aggtgggcac gggcttcacg ctgctgttca tcggcgccat cgtgttcatg
       61 gcgtacaagc gtctgccact catcgccttc accgtggtgt tcacgctgct gctcgccggc
      121 tacacctaca tcgcaccgcc cgccggcatc tggaaaggtt tcctgtgggt gctgctcgcc
      181 gcgctgtggc tgttcaacct gcgcccgctg cgcaaggcga tcatctcgcg gcccttcctc
      241 aaggcctaca tgcgcctgct gccctcgatg tcgcagaccg agaaggaagc gctcgaggcc
      301 ggcacggtgt ggtgggacgg cgagctgttc accggcaaac ccaagttcga gaaattgctg
      361 gccagcaagg cgccgcagct caacgccgag gaacaggcct tcatcgacgg cccgtgcgaa
      421 gagttgtgcc gcatgtacga cgactgggaa gtcacgcaca agctcggcga catgccgacc
      481 cacgtgtggg actacctcaa gcacaagggt ttcttcgcga tgatcatccc gaagaaatat
      541 ggcggcctcg aattttccgc ctatgcgcac tcctgcgtgc tggtcaaact agccagccgg
      601 tcgggattgc tcgcggccac caccgcggtg ccgaattcac tcggcccggc cgagctgctg
      661 aaccactacg gcaccgaaga gcagaagaac cactacctgc cgcgcctcgc gcgtggcgag
      721 gaagtgccgt gctttgcgct gactcacccg cgcgccggct cggatgccgc gtcgcttccg
      781 gacacgggag tcgtctgcaa aggcctgtac cagggcaagg agatcgtcgg gctcaagctc
      841 aacttctcga agcgttacat cacgctcgcg cccatcgcca cggtcgtggg cctggcgttc
      901 cggctctacg atccggacaa gttgttaggc ggcgacaagt ccgactacgg catcacctgc
      961 gcattgatac cccgcgcaac gccgggcctg atcatcggcc gccgtcactt cccgctcaac
     1021 gtgccgttcc agaatggccc gctctccggc aaggacgtgt tcgtcccgct cgacactatt
     1081 attggtggcc cgaaaatggc aggtcagggc tggcgcatgc tggtcgaaca gctttcggtc
     1141 ggccgctgca tctcgttgcc gtccaacgcg acgggcggcg ccaaggcggg cgtgttcgcc
     1201 tcgggcgcgt acacacgcat ccgccggcag ttcaacatgc cggtgggcaa gttcgagggc
     1261 gtagaggccg tcatcgcccg catggtgggc tacacctaca ccatggacgc ggcacgctcc
     1321 gtgaccgcag gcgccatcga tggcggcgag aagccgtcgg tgccttcggc catgctcaag
     1381 tatcacgtca ccgaaatggg ccgacaggtt tccaacgacg ccatggacgt tcacggtggc
     1441 aagggcatcc agttaggccc tcgcaactat ctcggccgca gctaccaggt ggtgcccgtg
     1501 gccatcacgg tcgaaggcgc gaacatcctc acgcgctcgc tcatcatctt tggccagggt
     1561 gcgatccgct gccatccgtt cgtactcaag gaaatgaacg ccgcgcgcaa cccggatcgc
     1621 aaacagggtg tcgaccaatt cgataccgcc ttgttcggcc acatcggctt caccatcagc
     1681 aacgccgttc gctcgttcgt catggcgctc acgcacgcgc gcttcacgca cgtgcccgac
     1741 gtgggcgaca ccaggcgtta ctaccagcac atcgtgcgtt tctcggcgtc gttcgcgttc
     1801 gccgtggacg tcgccatgct gacgctgggt ggttacctga agaagaagga gaacctctcc
     1861 gcgcggctgg gtgacgtgct gtcggcgatg tacctcgcat cgatggtgct caaacatcac
     1921 gagaaccagg gccgccccgc cgacgacctt cccgtggtcg aatgggcttg ccgcaatcag
     1981 ctctacaagg cgcaggacca gctgcacgat ttcctgcgca atttccccaa tcgcttcctc
     2041 ggctggatca tgcgcgcgct cattttcccg ggcggccgcg cctactccgc gcccagcgac
     2101 cgcctcggca gaacgctcgc cgacgcggtg ctcaatccga gcgaagtgcg cgaccgcctc
     2161 tgccgcttcg tctatcgcac cctggaaccc ggcaatccgc tcggcctgtt gcaggaagcg
     2221 ctggtgatgt cgcagacggc cgagcccatc gaaaagcgca tccgcgtcga cggcgtgaaa
     2281 accggcaagg tcaccgcgct cgatgtgccc gggcagatca cgcaggcgct gagcgaaggc
     2341 atcatctcgg acaccgaagc ggcgatgctg cgcgactacg atcgcaaggt catggagctc
     2401 atcagcgtcg acgacttcga atcgcacgag ctggcggcgc aggcggtaca gcctgacgac
     2461 aagacctccg gcagcgtcag cgatctcttt ccatccggcc aggtcgcctg acgggagacg
     2521 atgagctcac ctcccgttcc ggtagtcagt ccgccagaga cgccccagcc actgacgctg
     2581 gtgtgcgcga actgtcacgc gccgctgaac ggcgaatact gctcggcctg cgggcagcgg
     2641 cacgagccgc acatccacac cgtcgtgcac ttcgccggcg aggcgttcga gagtgtctcg
     2701 cacgcggact cgcgcctctg gcgcacgatc ggctatctac tgacgcgtcc cggctatctc
     2761 acgcgcgaat tttttgccgg ccgccgcgtc gcgtacctgc cgccgttccg gctttacctg
     2821 gtcatcagcg tgttgttctt cctggtcgcg ggcgtgccca aggacgccga gatcgtgcgc
     2881 atcgacgccg aagccacctc ggaaaccgcg ggtgccgacc agaaaccgaa agtcgccttc
     2941 agcatcgacg cgccgaaaga tgcggctggg gagctcaacg ccgccattgc cgaggcgaag
     3001 aaggccgccg ccgaaggcca gcccggggtc gacgtgtcgg acggcgtgcg cgacttctgc
     3061 gaccagttca aggaccaacc cacctcggac aacgaagcgc gcaacaacct gcgcgaccgt
     3121 tgtcgcgcca tcgccagcga cggcggacga gagctggcgg cgagcatcgt gcacaacgtg
     3181 ccgcgcgcca tgttcatctt cctgccgatc atcgcgttca tcatgaaact catgtactgg
     3241 cgcccgaagc gctattacgt cgagcacctg ctgttcctca tccacaatca cgcgttcgta
     3301 ttcctcgttc tcattccgct ggtgctcatc ggcaggattc cctacgtggg cgattactcc
     3361 ggctggctgt ggttcgccgc catcgtctac atgatctggt acacctatcg cggcatgcgc
     3421 aattactaca gtcagggccg cgcgctcacg ctcaccaagt atttcacgct cggtttttcg
     3481 tacctgatca cgaccttcgt cgtctttttg ctcgttgcca tctacagcgc gatgatggcc
     3541 tgaccgtcgc gcccccatcg aaggagatct ccatgaacaa ccggaaactg gtttcattcc
     3601 tctgcggcag cgcactcgca ttcggctcgc tgctggcgct cgcgcgcgag ccgtcgccgg
     3661 ccggcgccga ggtctacatc gtgtcgccga aagatggcgc gaaggtcaaa ggaccggtga
     3721 cggtggtgtt cggcctcaag ggcatgggca tcgcgcccgc cggcatcaag ttcgacaaca
     3781 ccggccacca ccatctgctc ctcgacgccg atgcgcccgc ggatctgtcg ctgcctctgc
     3841 ccgccgatcc gcacaacatt catttcggca agggccagac cgaaaccacg ctgacgctgg
     3901 cgccgggcac acacacgctg caactgttgc tggcggactc gctgcacacg ccgcacgatc
     3961 cggccgtgat ctcgaagaag atcacgatca cggtcgagaa atagcgcggg gagacgcggt
     4021 gaactggcgg attcattgcg ccgcggcggc gctggccaca ctcgcggctg ccacagcggc
     4081 tgcgcagcag gtgccggatc gcgaataccg gcccgccatc ggcaaacccg aatacgcgtc
     4141 gggccagggt ccggtagttt gcgtggacga agcgcaccac aacttccaca cgctggcgga
     4201 acgcttctgg gcgttcggct cgttgctgaa gcaggatgga taccaggtcg tgccgagtgt
     4261 ttcggccttc gatgcctcat ctctcgcgcg ctgcgccgta ctcgtgatct cgaacgcgca
     4321 accgagcgat gccgagtggg atcagtatcc ataccccacg ccatccgcgt tcaccgatgc
     4381 ggaaatcgcc gcggtgcatc gctgggtggg gaaagggggc gcgctgctcc tgatcgcgga
     4441 tcacatgccg ctggccgggg ccgcggcgaa actcgcggcg gcgttcgatg tcgagttcat
     4501 ggatggtttc gcggtacgcg atccgcagag ccgcgaaccc gacctctttc gcgcggccaa
     4561 tcagacgatg cgcgaccacg tgatcgtgcg tgggcgcaac gcgccggaat ccgtcgccag
     4621 catccgcacc tttaccggcc aggcatttcg cgcgccgaag gccgagccgc tgctggtgat
     4681 ccccgccggc tacgtggggc tcatgccgaa aaaggcctgg gagttcacgg ccgacacgcg
     4741 ccgcgtcgcc atcgaaggct ggctgcaggg tgcggtgcag gaagtcggca agggccgcgc
     4801 ggccttcttc ggcgaagcgg ccatgttttc cgcgcaactg gcgggcccgg acaagcagcc
     4861 catgggcatg aacgcgccgg gagcggaatc gaattttcga ttcacgctga atcttctgca
     4921 ctggctgacc cgaacgctgt aaaacggcgc catgagtgct ccgcgcgcca tcctgcatgt
     4981 cgacatggac gcgttttacg cgtcggtcga gcagcgtgac cggcccgaac tgcggggcca
     5041 gccgctcatc gtcggcggca ccagcaaccg cggcgtggtc gccgcggcga gctacgaggt
     5101 gcggaagttc ggcgtgcgct cggccatgcc gatccgcgag gcgctgcagc gctgtccaca
     5161 cgccatctgc gtgaagccgc gcatgtcggt gtaccgcgaa gtctcgcacc aggtcttcgc
     5221 cgtgtttcac gagtacacgc ccatcgtcga agggctttcg ctcgacgagg cttttctcga
     5281 cgtcaccgcc agcctctcgc tcaagggtga cgccatcacc atcgcgcgcg gcatcaagca
     5341 gcggatccgc gaagagacgc agctcgcggc ttccgtcggc gtggcgccta acaaactggt
     5401 ggcgaagatc gcctcggagc tggagaagcc cgatggcctc accgtcatcc atggcggcaa
     5461 ccttcgcgcg gttctcgatc cgctgtcggt gcgccgcctg ccgggtctgg gccggaagtt
     5521 aggcgagcgc gtcgaggccg ccggcctcgc caccttcggg caactgcgca ccgcgcccga
     5581 tacggtgttg tggccgattt tcggccgtga ctcccagcgc atgcgcgagc gcgcgtccgg
     5641 catcgacgaa cgtccggtga tgtcggagtg ggacgagaag tcgatcagcg ccgaggaaac
     5701 cttcttcacc gatctcgcgg atcactcgcg catgcattcg gaagtgctgc ggctcgcgga
     5761 tcgtgccggc acccgcatgc gcgcgcagaa cctcgccacc ggctgtgtcg cggtcaagat
     5821 ccggcgtgcc gacttcacca cgttcacgcg gcagagacgc ttcgagccct ccaccaccga
     5881 ttcgcgcacg atcgcgaaga tcgccgccga gctgctcacc gcgtggctgg cggagcaacc
     5941 cgcggcgcgc gtgcgattgt taggcgtcgg tgtgaatcac ctgcacgcgg ccgaccagat
     6001 ggacctgttc gcggcgcccg ccgctccggg ggcggcgaac accggcgcca ccgccctgga
     6061 cgccaccgtg gacctgatcc gcgagcgctt cggcaacctc gccctgcgac gcggaagcgc
     6121 cctgccggag gctcgcggcg aggaagaacc ggccgaggat atgtccgaaa aagcccccgg
     6181 cagaggtaag ccccgcgggc acgcgccaac gggcggccgc ggctagccct tccggtagcc
     6241 ggggtcaaaa agcccgtgta atactcccgg cccgttcaat cattgcggcg attcagcact
     6301 agaccgcata gccatgtatt cctccttctt cggcctaaac gagaagccct tctcgataac
     6361 ccccgacccg cgttacctgt tcctgtcgga acggcacgcg gaggcgctcg cgcacctggt
     6421 gtacggcgtg aacgaggccg gcggcttcat ccagctcacc ggagaggtcg gcaccggcaa
     6481 gaccacggta gttcggtcgc tgctggccca ggcgcccaaa cacgccgaaa tcgcactcat
     6541 cctgaatccg cgcatgacgc cggccgaatt cctgctggcc atctgcgaag agctgcggct
     6601 taccgtggct gcgggttcgg aacacagcat caaggatctc gtcgatctgc tgagccggca
     6661 cctgctcaag gcgcatgccg agggcaaacg catcgtgctc gtggtcgacg aagcgcagaa
     6721 cctgagcgtc gaagtgctcg agcaggttcg cctgctgacg aacctggaaa cagaaacgca
     6781 gaagctgctg cagatcatcc tgatcggcca gccggagctg cgtgagttgt tgggccgtgt
     6841 cgaactgcgg cagctcgcgc agcgcatcac cggccgttac cacctcgatc cgttgtcggg
     6901 tcccgaaacc gctgcctacg tgcgccaccg tctgcgcgtc gccggctcca cgcgcgaaat
     6961 cttctccaac ggcgccctgc gggaaattca acggctctcg ggtggcgtac cgcgtctcat
     7021 caacatcatc agcgatcgcg ccttgctggg cgcattcacc gaagatcgcc acctggtcaa
     7081 tgccggcgtc gtgcgccgcg ccgcgggtga agtgctcggc cgcagtttcc agccgcgctg
     7141 gctgccctgg accgccggcg cgggcgtggc cgccatcgcg atcgcaagcg cggtgcttat
     7201 gttgccgcgc ctcctgacta cccagacacc ggcgcccgtc gcggccgcgg cgcccgcacc
     7261 ggcgcctgcg ccggttgcgc cgccgccgaa tctcgaccag ttgttaggtc agtacccctt
     7321 ggaaaccgac cccgacaacg ccttcaacaa gttgtttgcg ctgtgggggc tgcggtacgt
     7381 ggccggcagc gtcgacccgt gcagccaggc gctgcaggcg ggtctcgagt gcctgacgca
     7441 acgcggttcg ctggcgcagc tgcggctctt caaccgtccc gccgtgctca acgtcatcga
     7501 ttccgcaggc catgcccacc agctcgtgct cgcgggtctc gacgacgaac acgcgcaggt
     7561 ggatctgggc ggcgcgcagc gcgaagtcgg catcggcgat ctttcgcgca gctggttcgg
     7621 cgactacgtg ttgttgtggc ggccggccac cggtggctcg cagccgctgg cactcggcgc
     7681 gcgtaacgca cgcgtgcgct ggctgcgcga cagccttcgt cgcgtcaatg gcctgccggc
     7741 cgatacggtt ccgaacgatc gcttcgacgc ggagctcgcg accctggtcg aggatttcca
     7801 gcgcaagaat cgcctcgccg tcgatggcat cgccggcgtg cagacccagg tcgcgctcga
     7861 tgccgcgctc aacgatgccg ccactccctt cctgcgcaac agcggcggta gctgacatgt
     7921 cgttcattct cgatgccctg aagaaatccg agaacgaccg ccagcgccag tcggcgccgg
     7981 cgctgttcga agtcaaggtg gccgcaccgc ggcgccgctt cccgctgtgg gccgccggac
     8041 tcgccatctt gttaggcgtc aatatcctcg cgctgctctg gttcgtgatg cgcgagcctg
     8101 cagcgccggt cgctccggct tccgccgcga ttgcgcctgc accagcaccc gccgcggcgc
     8161 cgcccggcat ggtcacggtt cccgcgacgt cgacttacat tcccgcgaat gctccgcccg
     8221 ccgtgaccgt cggaacggac acgggaatcg tggcgccgag caccgctccg ccgctcgccg
     8281 aggaacccat gttgtccgga caggaaccgt cggtgccgcc ggactacgat gcccgcgatt
     8341 accgtccggc gatcacgccc gatcaggcgg ctgcagtcgc ggccgcacgg cgcgaaggtg
     8401 ccgcgccgtc gcgcgacgag gtgctggcgg agggcacgca gcttccggac ctgcggctcg
     8461 atttgcacgt gtacgacccg gatccttcca agaaattcgt gttcatcaac atgcgcaagc
     8521 ttcgcgaggg tgaatcactg cccgaagggg tgcaggtgat ctcgatcacc cagacgggtg
     8581 cgcggctcac ttaccgcggc acgcagttca cgctcgaagc gaactagcgc tcaaccggcc
     8641 gcttctcggc ttcccaggca agatagtcgc cctcgaggtg cgccaggttc ttgaagcccg
     8701 ccttgcgcag gatgtcctcg gctaacaagg ttcgtcgccc gctgcggcag tagagcacca
     8761 cctgcttgtc gcgtgcgccc gccagctcgt cgaggcgcga agccaactgg tcgtgcgaca
     8821 cgttacgcgc gccgggcacg tgccccgcag cgaattcgtc ggcggtgcgc acgtcgagca
     8881 ccacgagatt cggatccttc tgctccaggc gcttgagcag ttccacctgc gtgatggggg
     8941 ccgcgttgga atcgtccgcg aacgcaggcg ttgcggcgaa cagcaatgct ccgactaaca
     9001 aagtaacgag gcgattcatg atcctctcct tggggtcagg cttcggcagc caggcgggcg
     9061 atctcgacag gcgtcagacg atggacgatc cattccttca tcggccgtgc atccagcgac
     9121 ttgtagaaac gcagcgcggg ttcattccag tcgagcacgg cccactccat gcgtccgcaa
     9181 ccgcgttctt ccgcgattcg cgccaacgcg cgcagcaacg cgcgtcctat tccatgcgag
     9241 cgctgctcgg gccgcacgaa caggtcttcg aggtacaggc ccggccggcc gacgaacgtc
     9301 gaaaagttga agaaatacag cgcgaacccc gcgggagcac cgtcccactc ggcgatcagc
     9361 acgtgaatga ccgggttggg agactcgatc tcccgcagga aatcttcggg cgtcgccttg
     9421 gcctcgtgca gcagcttttc gtagtccgcg agttcgcgca ccaggttcgc gatggtcggc
     9481 gcgtccgcct tcgtggcagg ccggatgttc agcattcgtt ccatcactcc gcgatgtacc
     9541 agcgcatgtc tttgtcacgc tcgagcacga agcgttcgta gggcagccct tgtccgctca
     9601 cgtcgtagat ggcccgcgct ccgcccgtgt cgtatttcgg ggagagctcc tgcacgacgc
     9661 gcagcgtggt gatcatggtc tcgcgcgtgc tctcgctgtt ggtgcaggcc gtgaccagcg
     9721 tcttgcgcgc ggatttcgac gtggcccggt tgaagttggg gctcatgaac tggctgtagt
     9781 actcctcgca atgaccctcg gcgaggctgg cctcggcgcg cgccagcagt tcgctgatac
     9841 ccggtgccag cggctgcggc cgttcttcga ccagcgaccg gcccggcgcg ccaccggtcg
     9901 gcggcggcgc cgcggcgacg cggccttcga tgagatcgtc gagttgcgct tcgacctcgg
     9961 tcggcacgat ggcgcgccac tgcgcgccgt actgggcgag cgcgaccgtc accaggactt
    10021 ttaccgcgcc acgctccttg ggctggatcc ccttgccgat gacatacacc accttgccgt
    10081 ccgcaacggt gccgagccag tcgaccttct cgtatagacg ggcgcgaaag cgcacgcgat
    10141 ccgacagggc ggcataaaag gccacgggcg tcatgcgctc cagttcggcc agcgacttgc
    10201 ccggtccaaa caaccgcgag cggtaggtgt tgtcggatcg cgaggcttcg gcgcgaaaca
    10261 gcgtgagcag gcgcgttcgc agcttttcca cttcgtcggg atgcagctcc tgggctacgg
    10321 acatgacgtt gccgccaccc agtgcagcca ggaatgcctc cgcaccacgc tgttgagccc
    10381 cgttagggcc cgcggcggac gcgggaaggc aggcggtcgc cgccaacacc gtcaaaatca
    10441 atgctctaag ggccatttga cgctccattc gaatacggcg gcggcgatca taaccggctc
    10501 gggaaccgcc gccccaactg tgaaagtggt ctcgttacga gacattggtg gcgggacgca
    10561 gttcacggat tgccggagcg aaaggctttc gcgcggcctt ccccgataaa ctgacatatc
    10621 ttgttaaagt gctttcgcac cctttgtttt cgctgctttt tgcgggtctg tcaccaccgt
    10681 catgttgccg cagtccgaat tcgaatcgcc catgctcgcc aacgccgcgg cgccggtggc
    10741 gattgccgcg attacgcagg atcgcgcact gatcggactg ctgcggtcgg tcatcgatcc
    10801 tgctaacgac ctcatcctgg tcacttccga agccgaactc actccgcatc tcaacagccg
    10861 ccgcgtttcc gtagcgctgc tcgacagcat gttcatcgaa ggcgatctcg cctcgatggc
    10921 cgagcgcttg cgcgagacct ggcccgatct cgtgctcgtg gtggtcggca ccgcggaaga
    10981 acaatcgaaa gtcgccggac agatcacctc cggcgtggtg tatcgctttc tgcatcgtcc
    11041 cgtgtccgcg ccgcgcgtgc gcttgttcgt cgacgccgcg ttgcgccgtc acgaagtcga
    11101 gaatgtcgag cgcacgctcg agcagactcg tccggatttc acccgtttcg aaaaggccgc
    11161 gaagaagacg ggcgcgccga gcccgatccg cgccgtgctg ccgggcatca tcggcgccgt
    11221 ggtcctcatt gccgccgccg gattctggtt cgcgcgtcca ggttccaggc aggaagccgc
    11281 gcccgaagcc gtcgcccagg tagatagtcc gccggccgac tccgcaccag ccgatacggc
    11341 ttccgccgaa ccggtcgccc agccggcccc gcccccgcgc gagtccgagc cgcaggcact
    11401 cgcgcctgaa cccgtatccg cacccgtggt cgcgcaggcc gcgccagatg aatccgtgag
    11461 cgagcgtctg cgacccgcca cttcgcaacc ggccgtcgta caggcacccg aagaactggc
    11521 gccacgcgtc ccgccgcagc cggttctctc attcgagcaa cgcctggccg agcagctcgc
    11581 gcatgccgaa tcagccctgc agcgcggcga actcgcctcg ccgcccggcc gcaacgccgt
    11641 cgaattgttc cgcggcgcac tcgacctcga ccctaccaac gcactcgcca agggtggcct
    11701 ggtgcgcgtg gccgacagac tgctgagtgc cgccgagcgc gcgatcaccg cgggcagcgt
    11761 cgaagacgcg aagaagatgg tggcggttgc cgaatcgctg acgcccgcga cggcgcgggg
    11821 cgctttcctg atgatgcaga tcgagcgcga gcaggaacgc gcggcgctca cgcaggccaa
    11881 gaacagcgac gcacaggaca agctggcgaa aggcgcgacg tatctgcggc tggcggagtc
    11941 gcgcctggcc agtggcgcac tcatcgaacc ttccggcgac aacgcacgct tctatttcga
    12001 agcagcgcgc caactcattc ccgatgatcc gggcctgcgc gaaaccgagc gtgcgctgca
    12061 gaaacagctg ctcgaccgcg cctcgaccgc ggccgacgcg ggcaacgcgg ccgagaccga
    12121 acgctggctg gccaacgccg atggcgccgg cgcaccgcgc gcggaaatgg cgagcattcg
    12181 ccgctcgatg cacgacacgc tgaatggcgc acgggccagc cgcgttacca ccctgacccg
    12241 ttccttcacc aacgcgctgg cggcgaaccg cctgctgcag cccgcggatg acaatgcgaa
    12301 ggcgcacctg ctggcgatga ccaacaccga tgccggcaat ccggccgttg ccacggcgcg
    12361 ccagagtctc ggcagcgcat tgctcggcca gggccgcgac gcgctgacgc ggggcgacct
    12421 gaccgcggcc gacacctggc tgaacgaggc tcgcagcatc ggcttcagca gcgacgaact
    12481 gaagaacgcc gaggccgaga tcgccacggc gcgcgaaaag gccacccagc gcaccgcggc
    12541 caccgccgtc gtcggcgaga aatcgctgga gcgcctggaa tacgtggcgc cgaaatttcc
    12601 ggccgccacg cgaaatcgcg gcgtcagcgg ctgggtcgaa ctggagttca cggtgctcgc
    12661 cgacggctcc accagcgacg tcgtcgtgac caactccaat ccgcgcaaga ctttcgatgc
    12721 ctcggcggtg accgccgtga gtcagtggcg ctacaagccc gtggtgcgct cgaacaaggt
    12781 cgtcgagcag cgagtcgccg tccgcatcag attctcggat caatagatgg acgcgccccg
    12841 cattcaacgt ttcctgatcg tcgagaccga cacacggtac gcgcagtggc tgcagcacac
    12901 ggtggaagca ggctggtcca acgattccat cgtcatcatg gactggagtt ctttcgcgcg
    12961 cgtgcgcacc gccatgaccg ttcgggacta cgacatcgtc atgttgtcga tggtcttcga
    13021 cgaaggcatc gaagatccga ccaccgacag tttcgactgg atccgcaagc tgcgcagcca
    13081 gccgaacttt ccggagatga tcatcctcgc cgatggtggc agcgagctgg ccgccatacg
    13141 cacgctgcgc ctgggcgccg cggactatct acccaagcgg ttgctgacgc cgccgcggtt
    13201 gcagcgctcc atcaagctca cgctgcgcgc catcgagaag gaagcgctgc gccgcgccga
    13261 gcgcaacgcc cagcctgccg cgaaaaccga aacggacaaa ctcgccgaga gcctga
//
LOCUS       DATDEU010000321         5663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_349190, whole genome shotgun sequence.
ACCESSION   DATDEU010000321 DATDEU010000000
VERSION     DATDEU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349190"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..503)
                     /locus_tag="VIV63_07680"
     CDS             complement(165..503)
                     /locus_tag="VIV63_07680"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEY6124517.1"
                     /translation="MSVQAKFENGARNAESTVIELNRIVAQAEELLKTLGEEGGAAVE
                     AVRQRVLRTVSQAKVKLADASTRARGAANEAADVTDRYVHNNPWKSIAYGAAAGAAVA
                     LIATALVRRD"
     gene            817..1221
                     /locus_tag="VIV63_07685"
     CDS             817..1221
                     /locus_tag="VIV63_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124518.1"
                     /translation="MIKTNSSWTLAAAAAVSIFALAGCNQRESANETAKDVAEARQDA
                     NANVAEEKREAGQVAMEGSEDRAAAEYDVAVAQADGQRKVAKEKCETLGADAQEACKD
                     QAEATYESAKAQAQATLDAAKRSGSAAPAPTN"
     gene            1353..2027
                     /locus_tag="VIV63_07690"
     CDS             1353..2027
                     /locus_tag="VIV63_07690"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY6124519.1"
                     /translation="MKRAPLKLLAAIAIAAPAFAVQAWAVSLPAAGPARSMPEDTANP
                     ASLVPVETWTAGSASLVPLMATDVQANQMTTVESPSTGNGGAMGKPLDDIAFVRQATE
                     SGRKEIDSARDALPQLQRPELKRIAGMLVSDHGGANARLTKIADAKGWPLPATESTDS
                     PPSGTASGDFDAKWTAEMIAGHEKSVALYRAQAQGGEDKDVRKYARDTLPTIEHHLAE
                     LRRLQK"
     gene            2024..2248
                     /locus_tag="VIV63_07695"
     CDS             2024..2248
                     /locus_tag="VIV63_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124520.1"
                     /translation="MTRTENSMLTMKIIKLLGIAGIAILAASFMAARVQRRDEEQRRR
                     LNERLEKDRWESEGGASRSGPATPSAVTAH"
     gene            complement(2317..3201)
                     /locus_tag="VIV63_07700"
     CDS             complement(2317..3201)
                     /locus_tag="VIV63_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY6124521.1"
                     /translation="MDHDEKVVQWFNAVDWIQAGSRIAAILAVSFLLVFIARRLISGL
                     EKFTATHAHDVEAQRRAATVSNVLRKLATILISCIAVLAIFNTLGISILPFLATAGVA
                     GIAIAFGAQSLVKDFFRGFFLLAENQIRQGDVVEVAGKSGLVEDLTLRYVQLRDYEGN
                     VHYVPNGEIGVVTSSSRTFAYAVVDVTIALEQEIAPVIEVMRETGAQLRANAEFGEKI
                     LEDLDIAGIEAWNDRGITLRARMKVRALEQSNVKREYLLRLKHAFDKADIQQPRATVT
                     IIDRGAPAKSTTPEGPPT"
     gene            3341..3685
                     /locus_tag="VIV63_07705"
     CDS             3341..3685
                     /locus_tag="VIV63_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124522.1"
                     /translation="MTSGNRVYLVSLALFGLLAAADAGRVGEDGTGAMWIAPELLEAA
                     SPAASVACTTPAVVHVTDLSSAGRVAFEWNPATSELSFHAQGIPRQVTLPEDWLSGAQ
                     ELSLATMSCGSR"
     gene            complement(3612..4139)
                     /locus_tag="VIV63_07710"
     CDS             complement(3612..4139)
                     /locus_tag="VIV63_07710"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY6124523.1"
                     /translation="MIADLFEVHVPVLELMLRGTLVYWLLFLIFRFLLRRDVGAVGIA
                     DILLLVIVADAAQNAMSGGYDTFAEGAILVSTIVARNWLLDVLAFRFSAVRRFASPGR
                     LTLIKQGVPQRRNLRREYITMDELFEKLREQGIEKMSEVKVAYLEGDGQISVIRNSSS
                     PETAPARRTASLPGA"
     gene            complement(4175..4444)
                     /locus_tag="VIV63_07715"
     CDS             complement(4175..4444)
                     /locus_tag="VIV63_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY6124524.1"
                     /translation="MGILLWLVIGGVVGWLASIVMKRDGSQGIILNVVVGIVGALLGG
                     WLLSPMLGAPSINSGVISMGSLLVSFLGAVILLAIVNLISRSRVR"
     gene            complement(4492..5538)
                     /locus_tag="VIV63_07720"
     CDS             complement(4492..5538)
                     /locus_tag="VIV63_07720"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6124525.1"
                     /translation="MSSNFFPGDNGAGLASSAYSALDDLDDCALRLIGNSAGMQKVRE
                     LITRVAPTDSAVLICGESGSGKECVAESIHAMSTRASKPLVALNCAAIPAALIEAELF
                     GHERGSFTGALRTREGVFERANGGTLLLDEITEMPLDLQSRLLRVLETKQLSRIGGSS
                     DVPVDVRVIASTNQFPSRAFEEGRLREDIYYRLAVLTIELPPLRDRGDDILTLADFFL
                     GALNMRNGTRKRLSSELRDQFMRHRWPGNVRELRNAVERAFILCDGELNLSEHEMPTL
                     RSPDSAAGGGDGIRVTIGTTLDEIERTAILATLKHFDGDKRRAADVLGCSLKTLYNKL
                     HAYQRERARQAAMN"
BASE COUNT         1053 a         1826 c         1793 g          991 t
ORIGIN      
        1 ctgctgcagg gccgtgggtc ctgacttcgc atcgcgcgtc ggcggcagcg gcggcgcctc
       61 ctgcggagcg ttggcaaaca gcaggaccaa cgcgagggtt tcgttcatct attcctccga
      121 agccgcggcg ccggtcatgt gccggacgcc gcgggatcct tcactcagtc gcgacgaacg
      181 agcgccgtgg cgatgagcgc aacagccgcg ccggcagccg cgccataggc gatcgacttc
      241 cacgggttgt tgtgcacgta acggtcagtg acgtccgccg cttcgttggc ggcgccacga
      301 gcgcgcgtgc tcgcatccgc gagcttgacc ttggcctggc tcacggtccg cagcactcgc
      361 tgccggactg cttcaacggc cgcgccgccc tcttcgccca gcgtcttcag cagctcttca
      421 gcctgcgcga cgatgcgatt gagttcgatc acggtggatt cggcgttgcg tgcgccattc
      481 tcgaattttg cctgtacgga catgaaactc ctccttaaac ggggttcagg gtgatggtga
      541 tgtgcctcgc gtaccttaag gcgtgtgcag gggcagcact gtcaggagat gcacggcatg
      601 tgctgccagc ggctcaccga aacctgtggg tcgacgctga cagagccggg atacgcagcc
      661 tgtcgccggg tggcgtcgtt tgcaggagat atcgcagtgc ggtcggtcgc gtctgacgcg
      721 cattacgcgt ttcgtccgcc tgaatggtcg atcgcagcgg agcaggatgc tcgcgacgaa
      781 tgattgccat tcgaaccatt ttcaggagat gtaacagtga tcaagacaaa cagcagctgg
      841 acattggcag cggccgcagc ggtaagcatt ttcgcgctcg ccggctgcaa tcagcgtgaa
      901 tcggccaacg agaccgcgaa ggacgttgcc gaggcgcgtc aggatgcgaa cgcgaatgtc
      961 gccgaagaga agcgtgaggc tggccaggtc gcgatggaag gctcggaaga ccgtgccgcc
     1021 gccgagtacg acgtggccgt agcgcaggcc gatggccagc gcaaggtcgc caaggaaaag
     1081 tgcgagaccc tgggcgccga tgctcaggaa gcttgcaagg accaggcgga agccacgtat
     1141 gaatccgcca aggcccaggc tcaggcgacg ctcgatgccg ccaagcgctc gggctcggcc
     1201 gctcctgctc cgaccaacta agagggcaca acacgcaatc gccagggtga tgcccacctg
     1261 cgagacctgc taattgtgat tgcgtggcag gccgcccggc gaagctaact acttcgccgg
     1321 gcggtcgact tatacgagga agtggaggaa acatgaaacg cgcaccgttg aaattgctgg
     1381 ctgcgatcgc catcgccgcg ccggccttcg ccgtgcaagc gtgggcggtc agtcttccgg
     1441 cggcgggccc tgcccgttcg atgcccgaag acaccgcgaa tcccgcctcg ctcgtcccgg
     1501 tcgaaacctg gacggcaggc agcgcctcgc tcgtgccgct gatggcgacg gacgtgcagg
     1561 ccaaccagat gactaccgtg gagtcgccgt cgaccggaaa cggcggagcc atgggcaaac
     1621 cgctcgatga catcgcgttc gtgcggcagg cgaccgaaag cggccgcaag gaaatcgatt
     1681 ccgcgagaga cgcgctgccg cagctgcagc gccccgaatt gaaacgaatc gccgggatgc
     1741 tggtgagcga ccacggcggc gcgaatgcgc gcctgacgaa gatcgccgat gccaagggct
     1801 ggccgttgcc ggccacggag tcgacggatt cgccgccctc gggcaccgcg agcggcgact
     1861 tcgacgcgaa gtggaccgct gaaatgattg ccgggcacga gaagtcggtg gcgctgtatc
     1921 gcgcccaggc ccagggcggc gaagacaagg acgtgcgcaa gtacgcgcgc gacacgctgc
     1981 cgaccatcga acaccacctg gccgagctca ggagattgca gaaatgaccc gcacggagaa
     2041 cagcatgctg accatgaaga tcatcaagct actgggaatt gccggcatcg cgatactggc
     2101 ggcgtcattc atggccgcgc gagtgcagcg ccgcgatgag gaacaacgcc gccgtctcaa
     2161 cgagcgcctc gagaaggatc gttgggagag cgaaggcggc gcgagccgtt ccggccccgc
     2221 caccccgagc gccgtcaccg cccactaacg gaaactgcga aaaggggaca gatctatttt
     2281 tccgcgaaac ggaaaaatag atctgtcccc ttttcgctag gtgggtgggc cctcgggcgt
     2341 agtcgatttt gccggcgcgc cccggtcgat gatggtgacc gtggcgcgcg gctgctgaat
     2401 atccgccttg tcgaacgcgt gcttcagccg cagcaggtat tcacgcttca cgttcgactg
     2461 ctccagcgcc cgcaccttca tgcgcgcgcg cagtgtgatt ccccgatcgt tccacgcttc
     2521 gattcccgcg atatcgaggt cttcgaggat cttctccccg aactccgcgt tggcgcgtaa
     2581 ctgcgccccg gtctcgcgca tgacttcgat gaccggcgcg atctcctgct cgagcgcaat
     2641 ggtgacgtcg accaccgcgt acgcgaacgt gcggctcgag ctcgtgacca cgccgatctc
     2701 gccattcggc acgtagtgca cgttgccttc gtagtcgcgc agctgcacgt aacgcagcgt
     2761 caggtcttcg accaggccgc tcttgcccgc cacttcgacc acgtcgccct gccggatctg
     2821 gttctcggcc agcaggaaga atccgcgaaa gaaatccttg accagtgact gcgcaccgaa
     2881 ggcgatcgcg atacccgcca caccggcggt cgccaggaac ggcaggatcg agatgcccag
     2941 cgtgttgaag atggccagca ccgcgataca gctgatcagg atggtggcca gcttgcgcaa
     3001 cacgttgctc acggtggcgg cgcgcctctg cgcttccacg tcgtgtgcat gcgtggccgt
     3061 gaacttctcg agcccggaga tcagccgtct tgcgatgaac accagcagga aggacaccgc
     3121 caggatcgcc gcgatacgcg agcccgcctg tatccagtcc accgcgttga accattgaac
     3181 gactttctca tcgtgatcca tcttttactc cgtgacctcg ggccctaccg gggcccgcac
     3241 ggtagcgtaa atggtcccgt ccgcgcatgc ggcatgttcc tacaccgtcc accgcttcgc
     3301 tccgaacggc aggccgcacg cgggaattca tgctcgggcc gtgacatcag ggaaccgggt
     3361 ctatcttgtt tcattggcat tgttcggcct gttagcggcc gcggatgccg gccgcgtggg
     3421 cgaagacggc acgggcgcaa tgtggattgc gccggaactg ctggaagctg cctcgcctgc
     3481 cgcgtcagtg gcctgtacga ctccggccgt cgtccacgtc acggatctga gctccgccgg
     3541 ccgtgtggca ttcgagtgga atcccgcaac cagcgagttg tcgttccatg cccagggcat
     3601 cccgcggcag gtcacgctcc cggaagactg gctgtccggc gcgcaggagc tgtctctggc
     3661 gacgatgagt tgcggatcac gctgatctgc ccgtcgcctt cgaggtacgc cactttcact
     3721 tcggacatct tctcgattcc ctgctcgcgc agtttctcga acaactcgtc catggtgatg
     3781 tactcgcgcc gcaggttgcg acgttggggt acgccctgtt tgatgagcgt caggcgtcca
     3841 ggggatgcaa agcggcgtac cgctgaaaac ctgaacgcca gcacgtcgag cagccagttc
     3901 ctcgcgacga tggtcgatac gaggatggcg ccttcggcga acgtgtcgta gccgccggac
     3961 atcgcgttct gcgccgcatc ggcgacgatg acgagcagga ggatatcggc gattcccact
     4021 gcgccgacgt cacggcgcag caggaagcgg aaaatcagga acagaagcca gtaaacgagc
     4081 gtgccgcgca gcatgagctc gagcaccgga acgtggactt cgaagaggtc cgcgatcaag
     4141 caccctcact catgccgagc gatacgcgat gcgctcagcg cacccggctg cgcgagatca
     4201 gattgacgat ggcgagcagg atcaccgctc ccaggaacga cacgagcagg ctgcccatgc
     4261 tgatcacacc cgaattgatg gacggtgcgc cgagcatcgg gctcaacaac caaccaccca
     4321 gcaatgcacc gacgatgccg acgacgacgt tcaggatgat gccctgacta ccgtcgcgct
     4381 tcatgacgat gctggcaagc cagcccacca cgccgccgat caccaaccac agtaaaattc
     4441 ccatctctct ctccttttgc gcgcggtcgc gcgcgtttta gttcgatccc ttcagttcat
     4501 cgcggcctgc cgcgcgcgtt cccgttggta cgcgtgcagc ttgttgtaaa gcgtcttgag
     4561 gctgcagccg agcacgtcgg ctgcgcgccg cttgtcgcca tcgaagtgct tgagcgtggc
     4621 gagaatggcg gtgcgctcga tttcatcgag cgtcgtgccg atcgtcacgc gaatgccatc
     4681 accgccgccg gccgcgctat cggggctgcg cagcgtgggc atctcgtgtt cgctcaggtt
     4741 gagttcgcca tcacacagga tgaacgcgcg ctcgacggca ttgcgcagct cgcgcacgtt
     4801 gcctggccag cgatgacgca tgaattgatc gcgcagctcg ctggaaaggc gtttgcgcgt
     4861 cccgttgcgc atgttgagcg cgccgaggaa gaaatccgcg agcgtcagga tatcgtcgcc
     4921 gcgatcgcgc agcggcggca gctcgatggt caggaccgcc agccggtagt agatgtcttc
     4981 tcgcaaccgg ccttcttcga acgcacgcga tgggaactgg ttggtggagg caatcacgcg
     5041 cacgtcgacg ggcacatcgc tcgacccgcc gatgcggctc aattgcttgg tctccagcac
     5101 gcgcaacagg cgtgactgga gatcgagcgg catctcggtg atttcgtcga gcagcaacgt
     5161 accgccgttg gcacgttcga agaccccttc ccgcgtgcgc aacgcaccgg tgaagctgcc
     5221 tcgctcatga ccaaacaact cggcttcgat gagcgcggcg ggaatggcgg cgcagttgag
     5281 tgccaccaac ggtttgctgg cccgcgtact catggcgtga atggactcgg ccacacactc
     5341 cttgccgctg ccgctctcgc cgcagatcag caccgccgaa tcggtaggcg ccacgcgagt
     5401 gatcagctct cgcaccttct gcatgcctgc agagttgccg atgagccgca gcgcacagtc
     5461 gtcgagatcg tcgagcgcgg agtacgcgct cgacgccaat cccgcgccgt tgtcgcctgg
     5521 aaagaagttg ctgctcacgg caccccggcc gcacgacccg gaatggcggc atcgccattc
     5581 gagacgatga tttccacccg gcggttcagt tgacggctgc ccgtgttcgc gttgctcgcc
     5641 acgggaaact cttcaccgta gcc
//
LOCUS       DATDEU010000322         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1955063, whole genome shotgun sequence.
ACCESSION   DATDEU010000322 DATDEU010000000
VERSION     DATDEU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955063"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VIV63_07725"
     CDS             <1..791
                     /locus_tag="VIV63_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6124526.1"
                     /translation="SRAIADLEQSVGLQLMKRTTRRIFLTAAGEAVLPHARAIGVEVE
                     KVRQLSQTFSTSREGPLRVIADPAYGRVLLAPLVPRFLDSFPDIALDVVLDSGEGADW
                     DVAIRAGVNEDPRLGARLLGAPPAVLCATPAYLLKHPAPDRPEGLRDHALLTPAGEGA
                     EYRFQMTKASARAEVRVRPKLAVDDPAVLHSATVAGLGIGLLPEFLCRQGVATAKLRR
                     VLADWQAPSAAPLYAVFPAGLENDHRVRSFVEFAAANIVPALAN"
     gene            814..>2609
                     /locus_tag="VIV63_07730"
     CDS             814..>2609
                     /locus_tag="VIV63_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY6124527.1"
                     /translation="MYRAPVKDIRFVLDELIGADLLRACPEFADYSSETADAVLGEAA
                     RFAEVVLEPICKTGDREGAKWSPNGVTMPEGYKDAYRQFCENGWPALRCKTEYGGQNV
                     PTMLGTAVEELWAASNLAFKLCPMLTQGAIEALQHFGSPQQKQLYLPKMVSGEWTGTM
                     NLTEPQAGSDLAQVRTRATPNGPNYRLYGQKIFITYGEHDMTENIVHMVLARIDGAPP
                     GVRGISLFIVPKFWVNADGLPGPRNDVNCASIEHKLGIHASPTCVMMYGENEGALGLL
                     VGEPNRGLEYMFVMMNAARLSVGLEGYAQGERAFQQAVDWARTRVQGKPPVPLARISG
                     SASMGTSSSPAPIIGHPDVKRMLLGMKSTVEACRALALYTAHQLDIGAAHPDEAVRAA
                     HQLRGDLLIPVVKGFCTESGIEIASLGIQVHGGMGYVEETGAAQTLRDVRITSIYEGT
                     TGIQSNDLIGRKFGRDRGAAMMAFIDEMEASLTALAPLHASALHARSAAQDALVRLRK
                     TSDSMLKALATAPDRAMAVSVPFLKFCGLAVGGWLMARAADIAAQRLVAGASDREFFE
                     AKLASAHFYSSQVLPQVLTLENICTQGSEAVSA"
BASE COUNT          448 a          876 c          849 g          436 t
ORIGIN      
        1 tcagccgcgc catcgccgac ctcgaacaat ccgtgggact gcagctcatg aagcgcacga
       61 cccgaaggat cttcctcacc gctgccggtg aagcggtgtt gccgcatgca cgggccatcg
      121 gcgtcgaggt cgagaaggta cgccagctct cgcaaacctt ctctacctct cgcgagggac
      181 ctctgcgcgt gatcgccgat cctgcctatg gccgcgtgtt gctcgcgccg ctggtgccgc
      241 gttttctcga cagctttccc gacatcgcgc tcgacgtcgt gctcgactcc ggcgaaggcg
      301 ccgactggga cgtggcgatc cgcgccggcg tcaacgaaga ccccaggctc ggcgcgcggc
      361 tgctgggcgc gccgccggcc gtgttgtgcg ccacgccggc ctacctgctg aaacaccccg
      421 cgccggaccg gcccgaaggc ctgcgcgatc acgccctgct cacgcccgcg ggtgaaggcg
      481 cggaatatcg ctttcagatg accaaggcct cggcgcgtgc cgaagttcgc gtgcgcccca
      541 aactcgcggt cgacgatccg gccgtgctgc acagcgcgac cgtcgcaggg ctcggcatcg
      601 gactactgcc cgaattcctg tgccgccagg gtgtggccac cgcgaagctg cgcagggtgc
      661 tcgccgactg gcaggcgccg tcagccgcgc cgttgtatgc ggtttttccg gccggcctcg
      721 aaaacgacca tcgagtgcgc agcttcgtgg aattcgctgc tgcgaacatc gttccggcgc
      781 tggcaaacta gccaaaaatc ttaaggagtg cagatgtacc gcgcaccggt caaggacatc
      841 cggtttgtgt tggatgagct cattggcgcc gatctgttgc gcgcctgccc ggagttcgcc
      901 gactactcga gtgaaaccgc cgacgccgtg ctcggcgaag cggcgcgatt cgcggaggtg
      961 gtgctggagc cgatctgcaa gaccggcgat cgcgaaggtg cgaaatggtc gccgaacggc
     1021 gtgaccatgc ccgaaggcta caaggacgct tatcggcagt tctgcgaaaa cggctggccg
     1081 gcgctgcgct gcaagaccga atacggcggt cagaacgtgc cgaccatgct cggtaccgcg
     1141 gtcgaagagc tctgggccgc gtcgaacctc gcattcaagc tttgccccat gctcacgcag
     1201 ggcgccatcg aggctctcca gcatttcggc tcaccacagc agaaacagct ctatctgccg
     1261 aagatggtga gcggcgagtg gaccggcacc atgaacctga ccgagcccca ggccggcagc
     1321 gatctcgcgc aggtgcgcac ccgcgccacg cccaatggcc ctaactaccg tctctacggc
     1381 cagaagatct tcatcaccta tggcgagcac gacatgaccg agaacatcgt gcacatggtg
     1441 ctggcgcgca tcgacggcgc gcctccgggt gtccgcggca tctcgctgtt catcgtcccc
     1501 aagttctggg tcaacgccga tggccttccg ggcccacgca acgacgtgaa ctgcgcgtcc
     1561 atcgaacaca agctcgggat ccacgcgagc ccgacctgcg tgatgatgta cggagaaaac
     1621 gagggtgcgc tcggcctgct ggttggcgag ccgaatcgcg ggctcgaata catgttcgtg
     1681 atgatgaacg ccgcgcgcct gtccgtgggc ctcgagggct acgcgcaggg cgagcgcgca
     1741 ttccagcagg ccgtcgattg ggcgcggacg cgtgtgcagg gcaaaccgcc ggtgccgctc
     1801 gccaggatct cgggctcggc gtccatgggc accagctcgt cgccggcgcc gatcatcggt
     1861 caccccgacg tcaagcgcat gttgttaggc atgaagtcca cggtggaagc gtgccgtgcg
     1921 ctggcgctgt ataccgcgca tcagctcgac atcggcgccg cgcatcccga cgaggccgtg
     1981 cgcgctgcgc accagttgcg cggtgacctg ctgattcccg tcgtcaaagg cttctgcacg
     2041 gagagcggca tcgagatcgc ctcgctcgga atccaggtcc acggtggcat gggctacgtc
     2101 gaggaaaccg gggccgcgca gacattgcgc gacgtacgca tcacctcgat ctacgagggc
     2161 accaccggca tccagtcgaa cgatctcatc ggtcgaaaat tcggccgcga ccgcggtgcc
     2221 gcgatgatgg cgttcatcga cgagatggaa gcgtcgctga cggcgctcgc gccgctgcat
     2281 gcatcggccc tgcacgcgcg cagtgcagcg caggatgctc tcgtcaggct gcgcaagact
     2341 tcggactcca tgctcaaggc gctggccacc gcacccgatc gcgccatggc cgtgtccgtc
     2401 ccgttcctca agttctgtgg gctcgctgtc ggcgggtggc tcatggcgcg agccgcggac
     2461 attgccgcgc aacggctggt ggccggcgcg agcgatcgcg aattcttcga agcgaagctg
     2521 gcgagcgcgc acttctactc gtcgcaggtg cttccccaag tgctgacgct cgagaacatc
     2581 tgcacgcagg gaagcgaggc ggtgtcggc
//
LOCUS       DATDEU010000323         3928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1955076, whole genome shotgun sequence.
ACCESSION   DATDEU010000323 DATDEU010000000
VERSION     DATDEU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955076"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VIV63_07735"
     CDS             complement(<1..752)
                     /locus_tag="VIV63_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6124528.1"
                     /translation="MQDERQREAAIVEAEMDARRAERLYQESDAMRWKLPAEVLSNAL
                     ERSAEKAFAGRAPSDTELDRYFDALHLADLALACACALGRDEAWDYFVREFRPGMYRA
                     ADAIDPSGGAREIAEALYAELFGLKELDGKRQSVFRYFHGRSSLATWLRSLIAQRHVD
                     RHRETRRLEPLPEDSSPASIPSQSKAEHPNRGRFVAAMHAVLSAAIGALAPRDRLRLA
                     CYYAQEMTLARIGRLTGEHEATVSRQLARTRRS"
     gene            797..1375
                     /locus_tag="VIV63_07740"
     CDS             797..1375
                     /locus_tag="VIV63_07740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY6124529.1"
                     /translation="MLNVGIILGSTRPNRNGEAVAKWVLEQTRSRKDASYELVDIKDF
                     ELPLLDEPIPASQGKYSQPHTKRWAEKIASFDAFVFVTPEYNHSTSGALKNAIDFLYR
                     EWNNKAAGFVSYGSAGGARAVEALRLIMAEIQIADVRAQVMFSLFTDFKNFSQFEPDP
                     RHDAELDAMLRQLVRWGNALKTVRSDAAKPAA"
     gene            complement(1439..>3928)
                     /locus_tag="VIV63_07745"
     CDS             complement(1439..>3928)
                     /locus_tag="VIV63_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6124530.1"
                     /translation="SFFARVGKQDDTINNPPQFPDLAPRRQRQFNNWGGAVGYDAVLS
                     PTLTNSFRYGFTKIDENNAGVTDANYVVFRFISPYEGKGDTNTFTDTRQTPTQNFVND
                     LSWFKGSHTMKVGTNLRFTRVPKNRFQASYLSATINPSWVAGIGRRNMPGSAFCTVPG
                     CSIPAVATAFQAGYADAWLNIIGVLSQATQRANYDQNGVPQAPGTAVAREYASDEYEW
                     YIQDAWQLRPNLTVTAGVRYSLYSPPYETNGLQVAPSVSMGQWFDQRAQNAANGIPST
                     ASEIVTFDLAGPKNNRPGFYAWDKNNFAPRLAVAWTPAERLVIRGGYSKVFDRVGVGL
                     ATTFDEGFAFGMSTQISSPFGAAYETNPAARFVNITTMPSTMPAAPAGGFPQTPPVRA
                     GIITSSIDDTLVTPSAHMTSATIGYDLSRNFSIEASYIGRHGRDMLVRRDLAMPLNLV
                     DPASRTDYFTAAQNIINAAQAAGIGSGSATGAYAGLPAVAYWENIFPGAAGGGLTATQ
                     AITRAFMQNGPDWITALYDMDTACSPACSKFGPYAYFAEQYDSLAAISSIGRANYNGM
                     NVSLRRRFADGVQFDINYTLAKSEDMGSQVERGGNFSSGYSNGGNTGFLINSFDPELN
                     YGRSDFDIRHQVNTNWLAELPFGQGKRYGGGVGDAVNAIIGDWSIAGLLRWTSGFPFN
                     VANCRSCWATNWNVQGNAMLVDPDRLPETETVENAVDGRPSPFVNATEAISFFRRQLP
                     GESGIRNLFTGDGYFNIDLSLSKAFRVGIADHRLRFRWDVFNVTNAAKFDVANLTNTP
                     DTAGFGRYNGTLATCDAQAGRCMQFALRYEF"
BASE COUNT          681 a         1239 c         1299 g          709 t
ORIGIN      
        1 gatcgccttg tgcgcgccag ttgacgcgac accgtggctt cgtgctcgcc ggtgagcctg
       61 ccaatccgcg cgagcgtcat ctcctgcgca tagtagcagg cgaggcgaag gcgatcgcgc
      121 ggtgccagcg cgccgatggc ggcggacagc acggcgtgca tcgccgcgac gaaacggccg
      181 cgattgggat gctcggcctt cgactgactc gggatcgatg cgggagacga gtcttcgggc
      241 aacggctcga ggcgccgtgt ttcgcgatga cgatcgacat gccgctgcgc gatcagcgag
      301 cgcagccagg tcgccaggct gctgcggcca tgaaaatacc ggaacaccga ctggcgtttg
      361 ccgtcgagct ccttcaggcc gaacagctcg gcgtaaagcg cctcggcaat ctcccgcgcc
      421 ccgccggacg ggtcgatggc atcggccgcg cgatacatcc ccggccggaa ctcgcgcacg
      481 aaataatccc acgcctcgtc gcggccgagc gcgcacgcgc acgccagcgc gagatccgca
      541 aggtgcaacg catcgaaata tcgatcgagc tcggtgtctg agggcgcgcg tcccgcgaat
      601 gctttctcag cgctgcgctc gagcgcattc gagagaacct cggcaggcaa tttccatcgc
      661 atcgcgtccg actcttgata cagccgctcg gcacgccggg catccatctc agcctcaact
      721 atagcggctt ccctctggcg ctcgtcttgc accaactgcg cgttgcgcct ccgatctgcg
      781 cgcgggagac agaatcatgc tcaatgtggg aatcattctc ggcagcacac gcccaaaccg
      841 gaacggtgag gcggtggcga aatgggtact cgaacaaacc aggagccgca aggacgcgtc
      901 ctacgagctc gtcgacatca aggatttcga gctgccgctg ctggacgagc cgatcccggc
      961 ttcgcagggc aagtactcac agccgcatac caagcgctgg gctgagaaga tcgcgtcgtt
     1021 cgacgcgttc gtgttcgtca cgcccgagta caaccattcg acctcgggcg cgctgaagaa
     1081 cgcgatcgat ttcctgtatc gcgaatggaa caacaaggcc gcgggtttcg tgagttatgg
     1141 cagcgccggc ggcgcgcgag cggtcgaagc gctgcggctg atcatggcgg agattcagat
     1201 cgccgacgtg cgcgcgcagg tgatgttctc gctgtttacc gacttcaaga acttctcgca
     1261 gttcgagccc gacccgcgtc atgacgccga gctcgacgcc atgctgaggc aactggtgag
     1321 gtgggggaac gccttgaaga ccgtgcgcag cgacgccgcg aagcctgcgg cctagcgccg
     1381 gaatgaagac gattgagacc caggcggccg aagccgcccg ggtccccgcc aaacccgctt
     1441 agaactcgta gcgcaacgca aactgcatgc agcggccggc ctgcgcgtcg caggtcgcca
     1501 gcgtgccgtt gtagcggccg aagccggccg tgtcgggagt gttggtcagg ttggcgacgt
     1561 cgaactttgc cgcgttggtg acgttgaaca cgtcccagcg gaatcgcagg cggtggtcag
     1621 caattccgac acggaacgcc ttgctcaggc tgaggtcgat gttgaagtag ccatcgcccg
     1681 tgaacaggtt ccggatgccg gactctcccg gcaactgtcg gcggaagaag ctgatggcct
     1741 ccgtggcgtt gacgaacggg ctcggccggc cgtcaaccgc gttttcaact gtctcggtct
     1801 ccggcaaccg atccggatcg acgagcatcg cgttgccctg gacgttccag ttggtggccc
     1861 agcacgagcg gcaattggca acgttgaacg ggaagccgct ggtccagcgc agcaggcccg
     1921 cgattgacca gtcgccaatg atggcgttga ctgcgtcgcc gacgccgcca ccgtatcgct
     1981 tgccttgccc aaacggcagt tccgcgagcc agttggtgtt gacctggtgc cggatgtcga
     2041 aatcggacct gccgtagttc agttccgggt cgaacgagtt gatcaggaat ccggtgttgc
     2101 cgccattgct gtagccgctg ctgaaattgc cgccgcgctc gacctgcgac cccatgtctt
     2161 cggacttcgc cagcgtgtag ttgatgtcga actgcacgcc gtcagcgaag cgccggcgca
     2221 gggacacgtt catgccgttg tagttggcgc ggccgatcga gctgatcgcg gcgagcgaat
     2281 cgtactgttc ggcgaagtat gcgtagggcc cgaacttgct gcacgccggc gagcatgcgg
     2341 tgtccatgtc gtaaagggcc gtgatccaat cgggcccgtt ttgcatgaac gcgcgggtaa
     2401 tcgcctgcgt ggcagtcagc ccaccgccgg ccgcgccagg gaagatgttc tcccaatacg
     2461 ccaccgccgg gagtccggcg taggcgccgg tcgccgaacc gctgccgatg ccggcagcct
     2521 gcgccgcgtt gatgatgttc tgggccgcgg tgaaatagtc cgtgcgcgac gcgggatcga
     2581 ccaggttgag cggcatcgcc aggtcacggc ggaccagcat gtcgcggccg tggcgtccga
     2641 tgtagctcgc ctcgatcgag aagttccggc tcaagtcgta gccgatggtg gcgctggtca
     2701 tgtgcgccga gggcgtcacc agcgtgtcat cgatgctcga cgtgatgata cccgcacgca
     2761 ctggcggggt ctgcgggaag ccacccgccg gcgccgccgg cattgtggac ggcatcgtcg
     2821 tgatgttgac gaagcgcgcg gcgggattgg tttcataggc ggcgccgaag gggctgctga
     2881 tctgcgtcga catgccgaat gcgaagcctt cgtcgaaggt cgtggccagg ccgacgccga
     2941 cgcgatcgaa caccttcgag tagccgccgc gaataaccaa ccgctccgcc ggcgtccacg
     3001 ccaccgcgag gcgcggcgcg aagttgttct tgtcccatgc gtagaagcct ggccggttgt
     3061 tcttcggtcc ggccagatcg aaggtgacga tctcgctggc ggtcgacggg atgccgttcg
     3121 ccgcgttctg tgcgcgctgg tcaaaccatt ggcccatgct gaccgagggc gccacctgca
     3181 agccattggt ttcgtagggc ggcgagtaga ggctgtaccg gacccccgcg gtcacggtca
     3241 gattgggccg cagctgccac gcatcctgga tgtaccactc gtactcgtcc gaagcatact
     3301 cgcgcgcgac cgccgttcca ggcgcctgtg gaacgccgtt ttggtcgtag ttggcccgct
     3361 gtgtcgcctg tgacagcacg ccgattatgt tcaaccaggc gtccgcgtag ccggcctgga
     3421 acgccgtggc gaccgccgga atgctgcagc ccgggaccgt gcagaacgcg ctgccaggca
     3481 tgttgcggcg gccgatgccg gccacccatg acgggttgat cgtcgcgctc aaatacgacg
     3541 cctggaaccg attcttcggc acgcgtgtga agcgaaggtt ggttccgacc ttcatcgtgt
     3601 gactgccctt gaaccacgac agatcgttga cgaagttctg cgtcggcgtc tggcgtgtgt
     3661 cggtaaacgt gttggtgtcg cccttgccct cgtacggcga gatgaaccgg aacaccacgt
     3721 agttcgcatc ggtgacgccg gcgttgttct cgtcgatttt cgtgaacccg tagcggaagc
     3781 tgttggtcag cgtcggcgac agcaccgcgt cgtaacccac cgcgccgccc cagttgttga
     3841 actgacgctg gcgccgcggc gccaggtccg gaaattgcgg cgggttgttg atggtgtcgt
     3901 cctgcttgcc gacgcgcgcg aagaagct
//
LOCUS       DATDEU010000324         2663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2101049, whole genome shotgun sequence.
ACCESSION   DATDEU010000324 DATDEU010000000
VERSION     DATDEU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101049"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VIV63_07750"
     CDS             complement(<1..435)
                     /locus_tag="VIV63_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY6124531.1"
                     /translation="MISQGDKVPGRKEHDSFGEIEVPADALWGAQTERARFSTMIFGG
                     QMPRDFIFAVAQIKGAAARANARLGLLPADIAGAIEGAVKDILNFRHQDQFPIDVFQT
                     GSGTSTNMNVNEVIAHLASRYAGVAVHPNDHVNMSQSSNDVIP"
     gene            475..1839
                     /gene="purB"
                     /locus_tag="VIV63_07755"
     CDS             475..1839
                     /gene="purB"
                     /locus_tag="VIV63_07755"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY6124532.1"
                     /translation="MDSAALLALSPVDGRYRKGADPLRAVLSESGLIRERVRIEAAWL
                     LSLAESVPQLLAQPLSDSARQVANTLALEPGEDAPAAVKAIESRINHDVKAVEYFVRD
                     KLAQGGATPAQLELVHFGCTSEDINNLAYARMLRAARGVMSGELEKVIDKLRQFAHEH
                     AALAMLSRTHGQTASPTTLGKEFANVVARLRRARARLDKVEILAKWNGAVGNYNAHVA
                     ALPHVDWPAVSRQFIESQGLVWNEFSTQIEPHDWIGEYCDALAGINTIIIDFARDTWG
                     YISLGYLRQRAVAGEVGSSTMPHKVNPIDFENAEGNLGVANALLHHFAAKLPISRWQR
                     DLTDSTVLRNVGVALAHSLIAWNSLQRGLGKIAADPKTIAADIERAHEVLGEALQTAL
                     RAAGIPNGYELLKDFTRGTQVDSAGLGAFIDKLPLPAPERARLKALAPQQYLGLATEM
                     ARSI"
     gene            2092..>2663
                     /locus_tag="VIV63_07760"
     CDS             2092..>2663
                     /locus_tag="VIV63_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124533.1"
                     /translation="MPDVSGEQNQIRVISSLEEMRAAVDAVAASAQRLMSIYTPDLEP
                     DLYDQNGFLEIVKRFVLARRFAKARVLLSDSGRLLRDNNRFIAMGRRLTSCIDIRPMA
                     GGAKQRAHAYLIADDRAIVYRVHPDRWDGVADLNNPPPHGDEAVVVAEQSSRVREQHA
                     GLGEAPRQHEAFDDLEKAVLVVQVGFEIGRV"
BASE COUNT          483 a          890 c          804 g          486 t
ORIGIN      
        1 cggaatcacg tcgttcgagc tctggctcat gttgacgtga tcgttgggat gcacggccac
       61 gccagcgtag cggctcgcga ggtgcgcgat cacctcgttc acattcatgt tggtgctggt
      121 gccgcttccg gtctggaaaa cgtcgatggg aaactgatcc tggtggcgaa agttcaggat
      181 gtctttcacc gcgccttcga tcgcaccagc gatgtccgcc ggcagcagac ctagcctcgc
      241 gttcgcgcgg gcggcggcgc ccttgatctg tgccacggca aagatgaaat cccgcggcat
      301 ctggccgccg aaaatcatgg tgctgaagcg ggcgcgctcg gtctgagctc cccacaacgc
      361 gtccgccggc acctcgatct cgccgaagct gtcgtgctct ttccggccag gaaccttgtc
      421 gccttgggat atcatgcgcg gccagtctat cgctcgtatc gaggcatgtc tttcatggat
      481 tccgcagcac tcttggcgct ctctcccgtc gacggccgtt atcgcaaggg ggccgacccg
      541 cttcgcgcgg tgttgtcgga aagcggcctc atccgcgaac gcgtgcgcat cgaagccgcg
      601 tggttgctgt cactcgccga atccgtgccg caactgctgg cgcaaccgct ctccgactcg
      661 gccagacagg tggccaacac gcttgcgctc gagcctggcg aagatgctcc ggccgcggtg
      721 aaggcgatcg agagccgcat caaccacgac gtcaaggccg tggaatattt cgtgcgcgac
      781 aagctcgccc aggggggcgc cacgcctgcc cagctcgaac tcgttcattt cggctgcacc
      841 tccgaagaca tcaacaacct tgcgtacgca cgcatgttgc gcgcggcgcg cggcgtgatg
      901 tcgggcgaac tcgaaaaggt catcgacaaa cttcgccagt tcgcccatga acatgccgcg
      961 ctcgccatgc tgtcgcgcac tcacggccag actgcgagtc cgactacgct cggcaaggaa
     1021 ttcgcgaacg tggttgctcg cctgcgccgg gcacgcgcgc ggctcgacaa ggtcgagatc
     1081 ctcgccaagt ggaacggcgc ggtcggcaac tacaacgcgc atgtggctgc gttaccccat
     1141 gtcgattggc cggccgtgag ccgccagttc atcgagtcgc agggcctcgt gtggaacgaa
     1201 ttctccacgc agatcgaacc gcacgactgg atcggcgaat actgcgacgc gctcgccggc
     1261 atcaacacca tcatcatcga cttcgcgcgc gacacgtggg gttacatctc gctcggctac
     1321 ctgcgccagc gcgccgtcgc cggcgaggtg ggctcgtcga ccatgccgca caaggtgaac
     1381 cccatcgact tcgagaatgc cgagggcaat ctcggcgtcg ccaacgcgct gctgcaccac
     1441 ttcgccgcca aactacccat ctcgcgatgg caacgcgacc tcaccgactc cacggtgctg
     1501 cgcaatgtcg gcgtcgcgct ggcgcacagc ctcatcgcct ggaattccct gcagcgtggc
     1561 ctcggcaaga tcgccgcgga cccgaagacg atcgcggcgg acatcgagcg cgcccacgag
     1621 gttctcggcg aagcgttgca gacggcgctg cgcgccgccg gcattcccaa tggctacgaa
     1681 ctcctcaagg acttcacgcg tggcacgcag gtcgattcgg ccggactcgg cgctttcatc
     1741 gacaaactac cgctgccggc gccggaacgc gcacggctca aggctcttgc gccgcagcag
     1801 tacctcggcc tcgcgacgga aatggcgcgc tcgatctaga gccccgctac ccataacggt
     1861 atgtgctgat gccgttacgt atatgtgacg cgtaaagtgc gaaccagcac acgtttttcc
     1921 gtcggtttcg cataaaggcc gatgaaagcg tgacgcattt ggcactggaa tacgcatgtg
     1981 ccccctagtc ttgatcgggc acaggtcaca aagcgaccta ggtctcacat gtcgaccggg
     2041 gaattagatt tcaacaaatt ttcgcagcgc gtttcagcgc cgggccacgc tttgcccgac
     2101 gtttccggcg agcagaacca gatccgggtg atcagctccc tcgaagaaat gcgtgccgcc
     2161 gtcgacgccg tcgcggccag cgcccagcgc ctcatgtcta tctacacgcc cgatctcgaa
     2221 cccgatctct acgaccagaa tggtttcctc gagatcgtca aacgtttcgt gctggcgcgc
     2281 cgtttcgcca aggcgcgcgt gttgctgtcg gattcgggcc ggctgctccg cgacaacaac
     2341 cgcttcatcg ccatggggcg gcggctcacg agctgcatcg acatccgtcc gatggcgggc
     2401 ggcgccaaac aacgtgcgca cgcctacctg atcgctgacg accgcgccat cgtctaccgc
     2461 gtacatccgg atcgctggga cggcgtcgcc gatctcaaca atccgccgcc ccatggcgat
     2521 gaagcggttg ttgtcgcgga gcagtcgtcc cgagtccgag agcagcacgc gggccttggc
     2581 gaagcgccgc gccagcacga agcgtttgac gatctcgaga aagccgttct ggtcgtacag
     2641 gtcgggttcg agatcgggcg tgt
//
LOCUS       DATDEU010000325         2484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_788970, whole genome shotgun sequence.
ACCESSION   DATDEU010000325 DATDEU010000000
VERSION     DATDEU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788970"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VIV63_07765"
     CDS             complement(<1..570)
                     /locus_tag="VIV63_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124534.1"
                     /translation="MDSLISTAGRALAAGDPLGALKLISLRDDAPALALRGIAMAQLG
                     EFARAKVLLRGAARAFGTKEAVARARCVVAETEVALASRDLGWRSHALESARATLESN
                     GDLANAAHARYLEVRRLLLIGHLDDAEAAVAELDATRLTPALRAAHELVIAGIAIRRI
                     RTKEARAALMRAERAARDAGIPALWAEVDV"
     gene            684..1409
                     /locus_tag="VIV63_07770"
     CDS             684..1409
                     /locus_tag="VIV63_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124535.1"
                     /translation="MSLLAMAAAMPSLAAETTLDHMPAALETQFALSAAPPGLRAKAS
                     VYLLDPKKGYYLAHQGSSGVACAVQRTQWEFADFRNDIYYGLCYDAAGVKTYLQVVMD
                     SAALRAQGMSPAALKAEIEKRFRDKTYLPPAKPGLSYMVGPIMRTIGPPDLKVKTMAM
                     PHLMFYAPYLTNEDIGATPSLNDRASLVYPFVDKQGIAEHTYMIQLIGEAETARILAD
                     EKPLVDALCAYRKLLCLAHDSHH"
     gene            1423..2184
                     /locus_tag="VIV63_07775"
     CDS             1423..2184
                     /locus_tag="VIV63_07775"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEY6124536.1"
                     /translation="MHTTGTRKEWLAARLDLLKEEKELTRRSDELSERRQSLPWVHID
                     KEYRFETDSGSASLKDLFRGRSQLMVYHFMFGPDYKAGCPSCSMIADGFEGFAVHLAN
                     HDVSLCAVSRAPLPKLQAYKRRMGWTFPWASSAGGEFNFDFNISFTEEEQRAGTIEYN
                     FNRGGHAMDASSKWPEPVVQFAATCGTDAPTYTRDRPGMSTFVMKDGAIYHTYSTYAR
                     GLDGLWGSYQWLDRAPLGRNETGVWWKRHDEYAKA"
     gene            2162..>2484
                     /locus_tag="VIV63_07780"
     CDS             2162..>2484
                     /locus_tag="VIV63_07780"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEY6124537.1"
                     /translation="MTNTRRPDRALLGTAALVFSASSAVTILWCGSMSAMPGMEMPGG
                     WTMSMAWMRMPGQSWSGAALTFIGMWAVMMVAMMLPVLVPMLMRYRRALREPRLRKGR
                     LTVIVA"
BASE COUNT          450 a          833 c          764 g          437 t
ORIGIN      
        1 cacgtcgact tccgcccaga gcgcgggaat gcctgcatct cgcgcagcgc gttcagcacg
       61 catgagcgcg gcgcgtgctt ccttcgttcg gatgcgccgg atcgcgattc ccgcgatcac
      121 gagctcatgc gcggcgcgta gcgcgggcgt aagtcgcgtg gcgtccagtt ccgcgaccgc
      181 ggcttcagca tcgtcgagat ggccgatgag cagcagccgg cgcacttcga gataccgcgc
      241 gtgcgcggcg ttggcgagat cgccattgga ctcgagcgtg gcgcgcgcgg attcgagcgc
      301 gtgcgagcgc cagccgagat cgcgcgacgc cagcgccact tcggtttcgg ccaccacgca
      361 tcgcgcacgc gccaccgcct ccttcgtgcc gaacgcgcgc gcggcgccac gcagcagcac
      421 cttcgcacgc gcgaattcgc cgagctgcgc catggcgatg ccgcgcaatg cgagcgcggg
      481 cgcgtcgtcg cgcagtgaaa tcagtttcag cgcgcccagc ggatcgcccg cagccagcgc
      541 gcgaccggcg gtggagatca gcgaatccat gcgcgacccg atgttttgaa tcccgacaaa
      601 cttgtcactc ccatctgttc gagcctcggg cctaaattta tcccatgaac aaacacccac
      661 acgcgattac tttactcgcc gccgtgagcc tgctcgcaat ggccgctgcg atgccctcac
      721 tggccgccga gacgacgctc gatcacatgc ctgccgctct cgaaacgcag ttcgcattga
      781 gcgccgcgcc gccgggattg cgcgccaaag catcggtata cctgctcgat ccgaagaagg
      841 gttactacct ggcccaccaa ggttcgagtg gcgtcgcctg cgcggttcag cgcacgcagt
      901 gggagttcgc cgatttccgg aacgacatct actacgggct ttgttacgac gccgcgggcg
      961 tgaagactta ccttcaggtg gtcatggact ccgccgcgtt gcgtgcgcag ggcatgagtc
     1021 cggcggcgct gaaagccgag atcgagaaac gctttcggga caagacctat ctgccaccgg
     1081 cgaagccagg cctgtcgtac atggtcggac ccatcatgcg caccatcggt ccgccggacc
     1141 tcaaggtgaa aacgatggcc atgccgcacc tcatgttcta cgcgccctat ctcaccaacg
     1201 aagacattgg cgcgacgcca tcgctgaatg atcgcgccag cctggtctat ccattcgtcg
     1261 acaagcaggg aatcgccgaa cacacctaca tgatccagct catcggtgag gctgagaccg
     1321 cgcggatact ggccgacgag aaaccgctcg tcgacgcgct ctgcgcctat cgaaaactct
     1381 tatgcctcgc ccacgattcg catcactaac cggagatttg atatgcacac cactggtacg
     1441 cgaaaagaat ggctcgccgc ccgcctcgac ctgctcaagg aggagaagga actcacacgc
     1501 cgcagcgacg agctgtccga acggcggcag tccctgccct gggtccatat agataaggag
     1561 taccgcttcg agaccgattc cgggtcggcc tccctcaagg acctgttccg cggccgctcg
     1621 cagctcatgg tttaccactt catgttcgga cccgactaca aggcgggctg cccttcgtgc
     1681 tccatgatcg ccgacggctt cgaaggcttc gcggtacacc tggccaacca cgacgtgtcg
     1741 ttgtgcgcgg tctcgcgcgc accgctgccg aagctgcagg cctacaagcg tcgcatgggc
     1801 tggacattcc cgtgggcatc gtcggccggc ggagaattca acttcgactt caacatctcg
     1861 ttcaccgaag aggagcaacg cgccggcacg atcgagtaca acttcaatcg cggcggccac
     1921 gccatggacg caagctcgaa gtggccagag ccggtggtgc agttcgccgc cacctgcggc
     1981 acggacgcgc ctacctacac gcgcgaccgc ccgggcatga gcacgttcgt gatgaaggac
     2041 ggcgctatct accacaccta ctccacctac gcgcgcgggc tcgacggact ctggggctcg
     2101 tatcagtggc tcgatcgcgc gccactcggc cgcaatgaaa ccggcgtctg gtggaagcgt
     2161 catgacgaat acgcgaaggc ctgatcgcgc gttgctcggc accgcggcgc tggtcttctc
     2221 ggccagctcc gcggtgacca tcctctggtg cggctccatg tcggcgatgc ccggcatgga
     2281 gatgcctggc ggctggacga tgtcgatggc gtggatgcgg atgccgggcc agtcatggtc
     2341 cggcgccgct ttgacgttca tcggcatgtg ggcggtgatg atggtcgcga tgatgttgcc
     2401 cgtcctcgtg cccatgctga tgcgttatcg ccgagcgttg cgcgagcccc gcctgcgtaa
     2461 gggcaggttg accgtgatcg tagc
//
LOCUS       DATDEU010000326         6882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1372248, whole genome shotgun sequence.
ACCESSION   DATDEU010000326 DATDEU010000000
VERSION     DATDEU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372248"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VIV63_07785"
     CDS             <1..382
                     /locus_tag="VIV63_07785"
                     /inference="COORDINATES: protein motif:HMM:NF020471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEY6124538.1"
                     /translation="AAWRRFKQQHTLSPLDKQVVSVISEHPEYHVIVESAAADLANYS
                     PRSGQLNPWLHMGLHLAIREQVSTNRPHGIADIHAKLAKQAGDAHEAEHRMLEILAEQ
                     IWEAQRAGKAPDENAYLERLRSIV"
     gene            complement(399..2477)
                     /locus_tag="VIV63_07790"
     CDS             complement(399..2477)
                     /locus_tag="VIV63_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124539.1"
                     /translation="MTRSIARLVLICLTATLPSGVLVAANAPGKEFHAPRGLCERVAR
                     LESSKSLQSILLQPATVDEAHGLRTYSIDINNDGRRDRLEVRRSAEDAHAELEVFDAS
                     GAALDFGEWNSNSNFADLGFLEHTGRHYVVVRNRASYPQRVRESLAYVARIGPDNVVH
                     PVCHFGMERSRSSHLPVGAAPVCQAVMEGRVHYTPFDTPFSFSPTQSQPDALLDYVPV
                     TDRAAIVDIDNDGDRESVARVEHSIQLSHGCGYHQLAVLDPQRTALQPDALPRPGNYE
                     GEVCADESLQPFVFEDATYIERRFNPAFPRDLHDVVQIKDGQWLTACSFDVRRRYVVA
                     SAFQVFAGDHEASLGYPRDDARDADEILELWRSLVEETDGKLADDMWKSEPGFRVALA
                     DPAQAYPILLTAIDAGRPEMVAWLLRRGVSPSVANGEESGGSSALEEAMRAESAEIVL
                     LLLRHGADPADAVSDLVSFAEDFPAEGRRALMLAVRRLKYLPDDVIRHAAENDPRLLS
                     ELASSGLPIERGDFGWQNGIEDPVDESSWTPGDDLSASGRASVLSSLRQLKRAAVRPG
                     SDSDVVAGYEVEGQSWVVQGSARSESDVENFLMFATSLCALHLPADCGPGTLLRKARR
                     WFRDLDECAPESRELYGERTCRLVDYARRVEADNEMSWRSFEGRTLAVKRPLGTWQDV
                     TLHYPAKDWP"
     gene            2607..3368
                     /locus_tag="VIV63_07795"
     CDS             2607..3368
                     /locus_tag="VIV63_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124540.1"
                     /translation="MSLSETIIAAIIGALATMLTAIVQLIRNRAPSETRPKKNRMRSL
                     MATIALMIGCVVGGYAWSSLRAVSAKDDLRMTLEAEFTKQFAAHAARQDPEGQAASAT
                     GSTNANGAAIPARNGEAGSAESLAHLPPCKINAQPDEVGPVTCTDRVAQSVALCAAIP
                     SAAHATNVRVQARVPKSESPWLERDAGAPTLGSLHVAAAPAEYPISADQRSVCLDVSN
                     WSVDDTLAVRVIVEYAFDGAPSAELTAAAPTAHSL"
     gene            complement(3372..4766)
                     /gene="lpdA"
                     /locus_tag="VIV63_07800"
     CDS             complement(3372..4766)
                     /gene="lpdA"
                     /locus_tag="VIV63_07800"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY6124541.1"
                     /translation="MLVLGAGPGGYTAAFRAADLGLKVTLVERWPMLGGVCLNVGCIP
                     SKALLHAAKVIDEVGEMAHHGVEYGAPKIDLAKLLEWKNSVVKKLVGGLSVLAKQRKV
                     EVVTGVGKFASPHVMEVTGADGKSQKIRFEQCIIAAGSEAIKLPFIPDDPRVIDSTGA
                     LELGGVPKRLLVIGGGIIGLEMATVYAALGAKLSVVELTGQLMPGADPDLVRPLEKRL
                     KARYEQILLNTKVTGCTAKPEGLAVDFEDASGKRTDIFDRVLVAVGRSANGKRIGLEN
                     ADIDVSDRGVIPVDKQMRTNLPHVFAIGDLVPGPMLAHKAMHEAKTAAEVAAGHKSYF
                     DARVIPSVAYTDPEVAWVGLTETDAKKNGTQYKKGQFPWAASGRSLALGRDEGFSKLL
                     FDPVSHRVLGGGMVGPNAGELVAEVALAIEMGADATDIGLTIHPHPTLSETVGLSAEA
                     FEGTITDLYMPKKA"
     gene            complement(4953..5168)
                     /locus_tag="VIV63_07805"
                     /pseudo
     CDS             complement(4953..5168)
                     /locus_tag="VIV63_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
     gene            complement(5165..6802)
                     /locus_tag="VIV63_07810"
     CDS             complement(5165..6802)
                     /locus_tag="VIV63_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEY6124542.1"
                     /translation="MSSVEVKVPDMGNFDTVTVIDVLVKPGDTIDIDTPLVTLETDKA
                     TMDVPSTAKGVVEKVHIAKGGAVSAGALIVTVKAEAGAAAPAPAAKPAAPTPAPAAAP
                     AAAPVAAATPTSIDVNVPDMGSFESVAVIDVLVKVGDTVDIDSPLVTLETDKATMDVP
                     STAKGVVEKVHVQKGGKLSPGALVVTVKGVAAVAAAAPSPAAPAATPDKPAAQAARPA
                     APQAVQQMMAPAARGGLPPIDEASFGAAHASPSVRKFARELGVNLGSVRGSGEKGRIL
                     LDDVKAFVKMVMAGGGPVAAAPALPKAHYADPSAFGAVEVKPLNRVQKISGPRLQAAW
                     INIPHVTQFDESDITDLETLRGSLKEKAAAAGVKVTPLAFIIEAAVRAMREFPVLNSQ
                     LDESGQNLIYKKFFNVGFAADTPNGLLVPVIKNADKLDVYAVAKALGDLSGKAREGKL
                     PGADMQGAGFTISSLGGVGGTMFTPIINSPEVAILGVSKSSMKPVWDGKQFVPRLMLP
                     LSFSYDHRVIDGAMGARIAKYLADTLANPKDLAGAMS"
BASE COUNT         1164 a         2330 c         2180 g         1208 t
ORIGIN      
        1 cgccgcctgg cgccgcttca agcagcagca caccctctcg ccgctcgaca agcaggtcgt
       61 gtcggtgatc tccgagcacc ccgaatacca cgtcatcgtc gaatcggccg ccgccgatct
      121 cgctaactat tcgccgcgca gcgggcagct caatccctgg ctgcacatgg gactgcatct
      181 cgcgatccgc gagcaggttt ccacgaatcg gccgcatggc atcgccgaca tccacgcgaa
      241 gctggcaaaa caggcgggcg acgcgcacga ggccgaacac cgcatgctgg agatcctggc
      301 cgagcagatc tgggaagcgc agcgcgccgg caaggcgccg gatgagaatg cttacctgga
      361 gcggctgcga tccatcgttt gatggcgctg gccatcactc agggccaatc cttggctgga
      421 tagtgcagcg tcacgtcctg ccacgttccg agaggacgct tcaccgccag cgtccgtcct
      481 tcaaaactgc gccaactcat ctcgttgtcc gcctccacgc gcctggcgta atcgacgaga
      541 cggcaggtgc gctcgccgta gagttcgcgt gactccggtg cgcattcatc caggtccctg
      601 aaccagcggc gcgcttttcg cagcagcgtg cccgggccgc aatccgccgg cagatgcagc
      661 gcgcacaacg atgtggcgaa catcaggaaa ttttcgacgt ccgattcgct gcgagcgctg
      721 ccttgcacga cccagctctg gccttccacc tcgtaaccag ccaccacgtc actgtctgaa
      781 cccggcctca ccgcggcgcg cttgagctgc cgcaggctgc tcaggaccga cgcacgcccg
      841 ctcgccgaga gatcgtcgcc aggcgtccag gagctttcat cgacgggatc ctcgattccg
      901 ttctgccacc cgaaatcgcc ccgttcgatc ggcaaaccac tcgatgcgag ctcgctcagc
      961 aaccggggat cgttctcggc cgcatggcga atgacgtcgt cgggcaagta cttcagtcgc
     1021 cgcaccgcga gcatgagtgc gcggcgtccc tccgcgggaa aatcctcagc gaagctcaca
     1081 aggtccgaaa ccgcatccgc cgggtccgcg ccatgacgca acagcagaag cacgatctcg
     1141 gcggactcgg ccctcatcgc ttcctcgagc gcgctgctgc caccactctc ttcgccgttc
     1201 gcgacgctgg gtgatacacc gcgccgcagc agccacgcga ccatctcggg ccggccggca
     1261 tcgatcgcgg tcaacaggat gggataggcc tgagcgggat ctgccaacgc gacgcggaag
     1321 ccaggctcgg atttccacat gtcatcggcg agtttgccgt cggtctcttc caccagactc
     1381 cgccacagct cgaggatttc atcggcgtcg cgtgcgtcgt cccgcggata gccgagactc
     1441 gcttcgtgat cgccggcaaa gacctggaaa gcgctggcga cgacgtagcg gcgccgcacg
     1501 tcgaagctgc aggccgtgag ccattgcccg tccttgatct gtaccacgtc gtgaaggtcg
     1561 cgcgggaacg cgggattgaa tcgccgctcg atgtacgtag cgtcctcgaa gacgaagggc
     1621 tgcaggctct cgtcagcgca aacctcgcct tcgtagttcc cggggcgcgg gagagcatcg
     1681 ggttgcagcg ccgtacgttg ggggtcgagt accgcgagtt ggtgataccc gcagccatgc
     1741 gacagctgga tgctgtgttc gacacgggcg actgactccc gatctccatc gttatcgata
     1801 tcgacgatcg ccgcccggtc ggtgacggga acgtaatcga gcaacgcgtc tggctgcgac
     1861 tgcgtgggag aaaatgaaaa cggagtgtcg aacggtgtgt agtgcacccg gccttccatc
     1921 actgcctggc agaccggcgc ggcgcccacg ggcagatggc tgctgcggct cctttccata
     1981 ccgaaatgac acaccgggtg aaccacattg tcgggcccga tccgggctac gtaggcgagc
     2041 gactcgcgca cgcgttgtgg atagctcgcc cggttgcgga cgacgacgta gtggcgtcct
     2101 gtgtgctcca ggaaacccag gtccgcaaaa ttcgaattcg agttccactc gccgaagtcg
     2161 agcgccgcgc ccgacgcatc gaacacttcg agctccgcgt gcgcgtcctc ggccgatcgc
     2221 cgaacttcca gccgatcgcg gcggccatca ttgttgatgt cgatggaata agtccgcagc
     2281 ccgtgagcct catcgacggt ggccggctgc aacaggatgg actgcagcga tttcgaggat
     2341 tcgagccggg cgacccgctc gcaaagtcca cgcggcgcgt gaaactcctt gccgggcgcg
     2401 ttggccgcga ccagtacgcc cgagggcagc gtggccgtca ggcagatcaa aaccaggcgt
     2461 gcgattgatc gggtcatggc agtcagacgg cacagcatcc ggaaatcttg gcgggaaagt
     2521 gtgaaccagt gcccacaaag gcagccctcc gcgctgccgc gcgacaggca cggactaaac
     2581 tcccctcact tgtttgaggt tccataatga gcctgtccga aaccatcatt gcggccatca
     2641 tcggcgcgct cgcgaccatg ctgacggcca ttgttcagct gatccgcaac cgcgcgccca
     2701 gcgaaacacg gccgaagaaa aaccgcatgc gctcgctcat ggcgaccatc gcgctgatga
     2761 tcggctgtgt cgtcggtggt tatgcgtggt cgtcgttgcg cgcggtgagt gccaaggacg
     2821 acctgcggat gaccttggag gccgaattca ccaagcagtt tgcggcgcac gccgcgcggc
     2881 aggatccgga aggccaggcg gcttccgcga ctggcagtac gaacgccaac ggcgccgcga
     2941 tcccggctcg caacggcgag gcgggctcgg ccgaatcact cgcgcacctg ccgccctgca
     3001 agatcaacgc ccagcccgac gaagtcgggc cggtcacctg caccgatcgc gtggcgcaat
     3061 ccgtagcgct gtgcgcggcg attccttccg cggcgcatgc caccaacgtg cgcgtgcagg
     3121 cgcgcgtacc gaagagtgaa tcgccgtggc tggaacggga tgctggggcg ccgacgctcg
     3181 gcagcctgca cgtggctgcc gcaccggccg aatatccgat cagcgccgat cagcgctcgg
     3241 tgtgtctcga cgtctcgaac tggagcgtgg acgacacgct ggccgtgcgc gtcatcgtcg
     3301 aatatgcgtt cgacggagcg ccgtccgcgg aactgaccgc ggcagcgccc actgcgcatt
     3361 ccctctagct actacgcctt cttcggcatg tagagatcgg tgatggtgcc ttcgaaggct
     3421 tcagccgaca gcccgaccgt ctccgacagc gtcggatgcg gatgaatcgt gaggccgatg
     3481 tcggtcgcgt ccgcgcccat ctcgatggcc agcgccactt cggcgaccag ctctccggcg
     3541 ttcggtccca ccatgccgcc acccagcacg cggtgcgaca ccgggtcgaa cagtagtttg
     3601 gagaagcctt cgtcgcggcc cagcgccagc gaacgcccgc tggcggccca cggaaactgg
     3661 cccttcttgt actgcgtgcc attcttcttc gcgtcggttt cggtcaggcc cacccaggcc
     3721 acttccggat ccgtgtacgc caccgacgga atcacgcgcg cgtcgaaata gctcttgtgg
     3781 cccgcggcga cttccgccgc cgtcttggct tcgtgcatgg ccttgtgcgc cagcatgggg
     3841 cccggcacga ggtcaccgat cgcgaacacg tgcggcaggt tggtgcgcat ctgcttgtcc
     3901 acggggatca cgccgcgatc gctgacgtcg atgtccgcgt tctcgaggcc gatgcgcttg
     3961 ccgttggcgc tgcgtcccac ggccaccagc acgcgatcga agatatcggt ccgcttgcca
     4021 ctggcatctt cgaagtccac cgccagccct tcgggcttcg cggtgcaacc ggttactttc
     4081 gtattgagca ggatctgttc gtaacgcgcc ttgagccgct tctccagcgg gcgcacgaga
     4141 tccgggtccg cgcccggcat gagctgaccg gtgagctcga ccaccgagag cttcgcgccc
     4201 agcgccgcgt acaccgtggc catctcgaga ccgatgatgc caccgccgat gaccagcagg
     4261 cgcttcggca ccccgccgag ttcgagtgcg cccgtcgaat cgatgacgcg cggatcgtcg
     4321 gggatgaacg gtagtttgat ggcctcggag ccggcggcga tgatgcattg ctcgaagcgg
     4381 atcttctgcg acttgccatc ggcgccagtc acttccatca cgtgtgggct ggcgaacttg
     4441 cccacgcccg tgaccacttc gaccttgcgc tgcttcgcga gcacagacag gccgcccacc
     4501 agcttcttga cgacgctgtt cttccactcg agcagcttgg ccagatcgat tttcggcgcg
     4561 ccgtattcca cgccgtgatg agccatctcg ccgacttcgt cgatgacctt ggcggcgtgc
     4621 agcaacgcct tggaaggaat gcaaccgacg ttgaggcaga cgccacccag catgggccag
     4681 cgttcgacga gcgtcacctt gagcccgaga tcggcagcgc ggaacgcggc ggtgtaaccg
     4741 ccgggaccgg cacccagcac taacaactgg gtttcgcgat cgtgcgggac atcgggcttg
     4801 agcggcctcg acggcttggg agctggcgca ggtgcggccc gcggcgcggg cgcagcggct
     4861 gcagtcggcg ccggcgcggg agcggccggt gcggcggcag cgggcgcctt cgcaggcgcc
     4921 gcggcagccg cggttccctc gatgcgcgcg atcaccccac ccttgccgat cttgccgccc
     4981 ttctgcacca gcacttccgt gacggtgcca gccgcagtcg acggcacatc catcgtggcc
     5041 ttgtcggttt cgagcgtgac cagcggcgtt tcgatttcta tcttgtcgcc caccttcacc
     5101 agcacgtcga tgattgcgac ctcgttgaaa tcgccgagat cgggaaccgt cagatcgatc
     5161 ttgctcacga catcgccccg gcgagatcct tcgggttggc gagcgtatcg gccaggtatt
     5221 tggcgatgcg ggcacccatc gcaccatcga tgacgcgatg gtcataggag aacgacagcg
     5281 gcaacatgag ccgcggcacg aactgcttgc catcccacac cggtttcatc gacgacttcg
     5341 aaacaccgag gatggcgact tcgggtgagt tgatgatggg cgtgaacatg gtgccgccca
     5401 cgccgccgag gctcgagatg gtgaatcccg cgccctgcat gtcggcgccc ggtagtttgc
     5461 cctcgcgcgc cttgccactc aggtcgccca gcgccttcgc tacggcgtac acatcgagct
     5521 tgtcggcatt cttgatgacc ggcaccagga ggccgttggg cgtatccgcc gcgaaaccca
     5581 cgttgaagaa cttcttgtag atgaggttct ggccggactc atcgagctgg ctgttgagca
     5641 cggggaattc gcgcatcgcg cgcaccgccg cctcgatgat gaacgccagc ggcgtgacct
     5701 tcacgcccgc agccgcggcc ttctccttga gcgagccgcg cagcgtctcg agatccgtga
     5761 tgtcggattc gtcgaactgc gtgacatgcg gaatgttgat ccacgccgcc tgcagacgcg
     5821 gaccggagat cttctgcacg cggttgagcg gcttgacctc gaccgcgccg aatgcggacg
     5881 ggtcggcgta gtgagccttg ggtaatgccg gtgcggcggc cacggggcca ccgcctgcca
     5941 tcaccatctt cacgaaggct ttgacgtcat cgagcaggat gcggcctttc tcgccgctgc
     6001 cacggacgct gccgagattc acacccagct cgcgcgcgaa cttgcgtacc gatggggaag
     6061 cgtgcgccgc gccgaaactc gcttcgtcga tgggcggcag accaccgcgg gccgccggcg
     6121 ccatcatctg ttgtacggcc tggggcgcag ccggtcgcgc ggcctgcgcc gcgggcttgt
     6181 caggcgtcgc ggccggagcc gctggcgagg gagccgccgc ggcaaccgcc gccacaccct
     6241 tgaccgtcac cacgagcgcg ccgggagaaa gctttccgcc tttctggaca tgcaccttct
     6301 cgacgacgcc cttggccgtc gacggcacat ccatcgtggc cttgtcggtc tcgagcgtaa
     6361 ccagcggcga gtcgatgtcg accgtgtcgc ccaccttcac cagcacgtcg atgaccgcga
     6421 ccgactcgaa gctgcccatg tcgggcacgt tgacgtcgat gctggtcggc gtcgcggcag
     6481 cgaccggcgc cgcggctggc gcggctgcgg gagcaggagt gggggccgcg ggcttggcgg
     6541 ccggcgctgg cgcggcagcg cccgcctcgg ctttcacggt gacgatcaac gcacctgcgg
     6601 acacggcacc gcccttggcg atgtgcacct tctccaccac gcctttggcg gtggacggca
     6661 catccatggt ggccttgtcg gtctccaacg tgaccagcgg cgtgtcgatg tcgatggtgt
     6721 cgccgggctt caccaacacg tcgatgacgg tgaccgtatc gaaattcccc atgtcgggga
     6781 ctttgacttc cacgctgctc atgctttctg cggatccagc ttgttggcgt cgaggcccag
     6841 tttgcccagt gcgctcttga gtgtgccctt gtcgagcgcg cc
//
LOCUS       DATDEU010000327         5589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_497991, whole genome shotgun sequence.
ACCESSION   DATDEU010000327 DATDEU010000000
VERSION     DATDEU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497991"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="VIV63_07815"
     CDS             <1..1749
                     /locus_tag="VIV63_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6124543.1"
                     /translation="KAHAEAGTLHGLFEASADKSGNAPAAYVGGWSVSYQDIEQQANR
                     IAQTLVARGVTPGAVVALCEEPGQSFLAGLLAILKTGGACLLLDPGVATARAASALSI
                     ARATAIVGNSRFKDIGAGRELTTLWLDADAAEIANASPARLAARASDTNAAAILLDLV
                     GSPMSPRIVTLSHATLTNTLLGVRDAVGITAQQRCLVAGALSAESTVLEWLLPWSAGA
                     ASVMVKNETLRDLDALQREIASGSVNFLHLPTEIWSALVARGFRPGNDLLGMVYGERP
                     SLELGEQLAGTGRIFRAWGFAEAGIWTTLSRIERAEDALLLGRPIDNVQVDVRDEQHL
                     TCPVNVWGELTLHVPGISSAPGPRVYGRWRTDGRLERQSQADDAVYAANSRIDLRAFQ
                     TLLTAQTGVDDAYCQLRFDAAARPVLVAHVAGQGNDLRDQLREVLAGQTPPVMSQSEV
                     IVLAKLPRTPDGRIDTRALPQGDAEASQGAANEFVAPATDKEKMLAEIWCDLLGLPEV
                     STRHNFFDLGGHSLLAMQAIATMEKKTGRQIAARRYMFQTLAQLAASYDEPAAEPPPK
                     QGLVGRLLGALRGGRK"
     gene            1760..2518
                     /locus_tag="VIV63_07820"
     CDS             1760..2518
                     /locus_tag="VIV63_07820"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124544.1"
                     /translation="MIPVRFGAETREMLGFYHAPARNVASAPAVLLCSPFGPEAIRSY
                     RMLRLLADRLANAGCAVLRFDYHGTGDSPGDDDVASLAGWAGDIGVAHRELAARSGKN
                     TVAWIGLRLGASLCALAAQTARPGLGLVVLWDPVIVGIPYLEIYRSHSGPNIGVAFET
                     MGFEFSDRFRNDLSTLNLSQLTLPSGARVSAIVGSEDSHATALRESLTRSEVRLDWTV
                     SPAVAEWNSEKALNSAVIPMQLLQGIESKVMEPV"
     gene            2515..3360
                     /locus_tag="VIV63_07825"
     CDS             2515..3360
                     /locus_tag="VIV63_07825"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124545.1"
                     /translation="MSETTLAFGANSGLMGTLSAPPQPSAGIGALLFNAGVVPRIGPN
                     RLNVRIARALQARGIPALRFDLSGRGDSAPARGMESYEQQAVTDIRAAMDLLHERTGV
                     NRFAIMGLCSGAENAFHAALADERVIGVALMDSYHYPTLRTHFNRFRQRASMQGGLFG
                     AGAAWIRRRVRRVTNGAARAREGASNSFGSIRPTPEAFAGQLGKLIARGVKIDLLFSG
                     SFLETYNYENQFRDGFGRFGIVDHMHIEYRPELDHTLSTAIMQNEMVTRTARWCSALS
                     EKPAP"
     gene            complement(3411..4082)
                     /locus_tag="VIV63_07830"
     CDS             complement(3411..4082)
                     /locus_tag="VIV63_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY6124546.1"
                     /translation="MQTKKYIGLVAVAALGFGSAAAQAALVDRGGGLIYDDVLNVTWL
                     QDARYAVTTGAAPFGSLTWQDAKDFADGMSYVDEVRGVVWDDWRLPQVFDAGAEGYDV
                     LGQSSELAYMYYINLGYAPNYDPHTTDPVPESSAYNPFINISYRGYWTGNEAPFAGRA
                     WYEHFHFGFGGVTGQGDYNQVWAVRDGDVGAAVSAVPLPGTLGLFGAALGLAGFIRRR
                     KPALA"
     gene            complement(4228..4662)
                     /locus_tag="VIV63_07835"
     CDS             complement(4228..4662)
                     /locus_tag="VIV63_07835"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6124547.1"
                     /translation="MSDFRLDHVCLAVRKIAPARAMLEKMLGYQPRTEVVHNTRQRVN
                     VQFMSKAGSLDIKLIEPDSLDSPLVDFIKSRGGGLHHLAFSTESVEEGVNDLAARQAR
                     IVAPPAPGEAFDDETIAFAFLGAGLNIELIDTDRRRAEIDSK"
     gene            complement(4659..>5589)
                     /locus_tag="VIV63_07840"
     CDS             complement(4659..>5589)
                     /locus_tag="VIV63_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6124548.1"
                     /translation="RGLMLYGSLHLPDAATSPGTHKDLPVVVLLSPGVKMRVGPGRLY
                     VPLTELLTSQGFRVLRFDFFGLGDSEGDLDETVLADVYNNIEVGRYVDDTLAALNWLA
                     SEHGAKRFVLGGLCGGAITAILAAERDVRVDSLLSIGMTVTLASNAAKPGQYLTRAQL
                     DDRRRRYLLKMFKPEAWWRFITFRSEYDVIWKAMKRAFIKDKPVAAAQVTLGTAAPPV
                     EQRGNANPLFPKAYFTFLARGGKALMLFSERDRLQSEYEEKFAQPFAAQLAPHAGQIE
                     QHVIPHANHVLSFREWQEQMLALSRSWLARLYT"
BASE COUNT          943 a         1864 c         1839 g          943 t
ORIGIN      
        1 aaggcgcacg ccgaagccgg cacgctgcat ggcctgttcg aagcgtccgc cgataaatcc
       61 ggcaacgcac cggcggccta cgtcggcggc tggagcgttt cctaccagga catcgaacaa
      121 caggcgaacc gcatcgccca gactctcgtc gcgcgcggcg tgacgccggg cgcagtcgtt
      181 gcactgtgcg aagagccggg gcagagcttc ctggccgggt tgttagccat cctcaagacc
      241 ggcggcgcct gcctgctgct cgatccgggc gtcgccacgg ctcgtgccgc cagtgcgctg
      301 tcgatcgcgc gcgccacggc aatcgtcggc aattcgcgat tcaaggacat cggcgcgggc
      361 cgcgaactca cgacgctctg gctggacgcg gacgccgccg agatcgccaa tgcttcgccc
      421 gcgcggctgg ctgcgcgcgc ctccgatacg aatgccgccg cgatcctgct cgacctcgtc
      481 ggcagcccca tgtcgccgcg catcgtgacg ctgtcgcatg ccacgctgac caacacgttg
      541 ctgggcgttc gcgacgcagt gggtatcacc gcgcagcagc gctgcctcgt ggccggcgcc
      601 ttgtcggcgg aatcgacggt cctcgaatgg ctgttgccct ggagcgccgg cgccgcgagc
      661 gtcatggtca agaacgagac gctgcgcgat ctcgacgcct tgcagcgcga aatcgcgagc
      721 ggcagcgtga acttcctgca cctgcctacc gagatctgga gcgcgctcgt ggcgcgtggc
      781 ttccgtccgg gcaacgatct tctcggcatg gtctacggcg agcgtccgtc gctcgagctc
      841 ggcgaacaac tcgcgggcac cggccgcatc ttccgcgcct ggggattcgc cgaagcggga
      901 atctggacca cgctgtcgcg catcgaacgc gccgaggacg cgctgttgtt aggccgcccg
      961 atcgacaacg tgcaggtcga cgttcgcgac gaacagcacc tgacctgccc cgtcaatgtc
     1021 tggggtgagc tcacgctcca cgtcccgggc atttcatcgg cgccgggtcc gcgcgtgtac
     1081 ggtcgctggc gcaccgatgg ccgcctcgaa cggcagtccc aggccgacga cgccgtctac
     1141 gccgcgaatt cgcgcatcga cctgcgcgcg ttccagactt tgctcacggc gcaaaccggt
     1201 gtcgatgacg cgtattgcca gttgcgattc gatgcggcgg cacgtccggt gctggtcgcc
     1261 cacgtcgccg gccagggcaa cgacctgcgc gaccagttgc gcgaggtgct cgccgggcag
     1321 actccaccgg tgatgagcca gagcgaagtc atcgtgctcg ccaaactgcc tcgcacgccc
     1381 gacggacgca tcgacacgcg cgcgctgccg caaggcgacg cggaagcgtc acaaggcgcc
     1441 gccaacgagt tcgtcgcgcc ggccaccgac aaggaaaaga tgctcgcgga gatctggtgc
     1501 gatctgctcg gcctgcccga agtcagcacg cgccacaact ttttcgactt gggcggtcac
     1561 tcgctgcttg ccatgcaggc gatcgcgacc atggaaaaga agaccggccg gcagatcgcc
     1621 gcgcgccgct acatgttcca gacgctggct cagctcgcgg cgtcttacga cgaaccggcc
     1681 gcggaaccgc cgccgaagca gggcctggtg ggccgactgc tcggcgcgtt gcgaggcgga
     1741 cgcaagtgag tcgctggaag tgatacccgt ccgcttcggc gcagaaaccc gcgagatgct
     1801 gggtttctat cacgcgccgg cgcgcaacgt ggcgagcgcg ccggcggtgt tgctatgcag
     1861 ccctttcggg cccgaggcca tccgcagcta ccggatgctc cgcctgctcg ccgaccggct
     1921 ggccaacgcc ggctgcgccg tgctgcgctt cgattaccac ggcaccggcg actcgccggg
     1981 cgacgacgat gtcgcatcgc ttgcgggttg ggccggtgac atcggcgtcg cgcatcgcga
     2041 gctcgccgcg cgctcgggca agaacaccgt ggcctggatc ggcctgcgtc tgggcgcctc
     2101 gctgtgcgcg ctcgccgcgc agaccgcccg accaggcctt ggcctcgttg tcctgtggga
     2161 cccggtgatc gttggtattc cctacctcga aatctatcga agccattcag gacccaatat
     2221 cggcgtggca ttcgagacca tgggtttcga attctccgat cgcttccgca acgatctctc
     2281 cacgctcaac ctgtcccagc tgactttgcc gtcgggtgcg cgcgtatcgg ccatcgtcgg
     2341 cagcgaggac tcccacgcga ccgcgctcag ggagtcgctc acacgctccg aagtccgcct
     2401 cgactggaca gtgtcgccag cagtggcaga gtggaattcc gaaaaggcac tcaacagcgc
     2461 ggtcattccc atgcagctcc tgcagggcat cgaatcgaaa gtgatggagc ccgtgtgagc
     2521 gaaaccaccc tggcgttcgg cgccaactcg ggactgatgg gaacgctcag cgcgccgcca
     2581 caaccgtcgg ccggcatcgg cgcattgttg ttcaacgccg gcgtggtacc ccgcatcgga
     2641 cccaaccggc tgaacgtgcg catcgcgcgc gcgctgcagg cgcgtggcat tcccgcgttg
     2701 cgcttcgatc tttccggccg cggcgacagc gctccggcgc gcggcatgga gtcatacgaa
     2761 caacaggccg tgacggacat ccgcgcggcc atggatctgc tgcacgagcg caccggcgtc
     2821 aaccgcttcg cgatcatggg gctctgctcc ggcgcggaaa atgcgtttca tgcggcgctc
     2881 gccgacgaac gggtgatcgg cgtggcgctg atggactcgt accactaccc gacgctgcgc
     2941 acgcacttca accgcttccg ccagcgcgcc agcatgcaag gcgggttgtt tggcgcaggt
     3001 gccgcctgga tccggcggcg ggtgcgacgg gtcacgaacg gcgccgcgcg cgcgcgcgag
     3061 ggagcgtcga acagtttcgg atccatccgg ccgacgccgg aggctttcgc cgggcagctc
     3121 ggcaagctca tcgcccgcgg cgtgaagatc gacctgctgt tctcgggcag ctttctcgag
     3181 acctacaact acgaaaacca gtttcgcgac gggttcgggc gtttcggcat cgtcgaccac
     3241 atgcacatcg aatatcggcc cgaactcgat cacacgctct cgacggccat catgcagaac
     3301 gagatggtca cgcggaccgc gcggtggtgc tcggccctct cggaaaagcc ggcgccataa
     3361 aaaaggcccg ccagaggcgg gcctttgagt cgcaagaact ctctttgttt ttaggcaagg
     3421 gccggcttgc gccggcggat gaatccagca agtcccagcg cagcgccgaa gaggcccagc
     3481 gtgccgggca gcggtaccgc cgagacggca gcgcccacgt cgccgtcacg aacagcccag
     3541 acctggttgt agtcgccctg gccggtgacg ccgccaaatc cgaagtggaa gtgctcgtac
     3601 caggcacggc cagcgaacgg ggcttcgtta ccggtccaat agccgcggta gctgatgttg
     3661 atgaacgggt tgtacgcgct cgactccgga accgggtccg tggtgtgcgg atcgtagttc
     3721 ggggcgtagc cgaggttgat gtagtacatg taggcgagct cgctgctctg cccgagcacg
     3781 tcatagcctt cagcgccggc gtcgaacacc tgcggcagac gccagtcgtc ccagaccaca
     3841 ccacgcactt catcgacgta gctcatgccg tccgcgaagt ctttcgcgtc ctgccaggta
     3901 aggctcccga aaggcgccgc gcccgtggtg accgcgtagc gcgcgtcctg gagccaggtg
     3961 acgttcagga cgtcgtcata gatgaggccg ccgccgcgat cgacgagggc tgcctgggcg
     4021 gctgcggaac cgaatccgag cgctgcgact gccacaaggc cgatgtactt cttcgtttgc
     4081 aatttctctc tcccttctcg ccggtctacg acgtgcagtg aatctatccg tgcgtgcccg
     4141 gcgcaggggg tcacggcggg ggtgcgatta gggaaagtgt gacgtaggtc atacgcggat
     4201 ttgctgaaca taatgcaacc gcgacagtta cttggagtcg atttccgccc gcctgcggtc
     4261 ggtatcgatg agctcgatgt tgagcccggc gccgagaaac gcgaatgcga tggtttcgtc
     4321 gtcgaaggct tcgccaggag ccggcggagc gacgatgcga gcctgcctgg cagcgaggtc
     4381 gttcacgccc tcctcgaccg actccgtgct gaaggcgagg tgatgcagcc cgccgccgcg
     4441 gctcttgatg aaatcgacca gcggcgaatc cagcgaatcc ggctcgatga gctttatgtc
     4501 gagcgagccc gccttggaca tgaactgcac gttcacgcgt tggcgggtgt tgtgcacgac
     4561 ttccgtacgc ggctggtaac ccagcatctt ctcgagcatg gcgcgcgccg gcgcgatctt
     4621 gcggaccgcg aggcacacgt ggtcgagcct gaagtcgctc atgtgtacag ccgggcgagc
     4681 caggagcggc tcagcgccag catctgttcc tgccactcgc ggaacgacaa cacgtggttg
     4741 gcgtgcggaa tgacgtgttg ctcgatctga cccgcgtgcg gcgcgagttg cgccgcgaac
     4801 ggctgcgcga atttttcttc gtattcggac tgcaggcggt cgcgctcgct gaagagcatg
     4861 agcgccttgc cgccgcgcgc gaggaacgtg aagtacgcct tggggaacag cggattcgca
     4921 ttgccgcgtt gttcgaccgg cggcgcggca gtgcccagag tcacctgcgc ggccgcgacc
     4981 ggcttgtcct tgatgaacgc gcgcttcatg gctttccaga tcacgtcgta ttcgctgcgg
     5041 aacgtgatga aacgccacca cgcctccggc ttgaacatct tgagcaggta gcggcgccgg
     5101 cgatcgtcga gctgcgcgcg cgtcaggtac tggccgggct tcgcggcatt actcgcgagc
     5161 gtcaccgtca tgccgatcga cagcagcgag tcgacgcgga catcgcgctc cgccgccagg
     5221 atggccgtga tggcgccgcc acacaggccg ccgagcacga agcgtttcgc gccgtgttcg
     5281 gacgccagcc agttcagcgc ggcgagcgtg tcgtcgacat agcgccccac ttcgatgttg
     5341 ttgtagacat cggcgagtac ggtctcgtcg agatcgcctt ccgagtcgcc gaggccgaag
     5401 aagtcgaagc gcaacacgcg aaagccctgc gatgtcagca gttcggtgag cggaacatag
     5461 agacgtccgg gcccgacgcg catcttcaca cccggcgaca gcaacaccac gaccggcaga
     5521 tccttgtgcg tgccggggga cgttgcggca tcgggcaggt gcaggctgcc gtagagcatg
     5581 agcccgcgc
//
LOCUS       DATDEU010000328         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1373054, whole genome shotgun sequence.
ACCESSION   DATDEU010000328 DATDEU010000000
VERSION     DATDEU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373054"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VIV63_07845"
     CDS             complement(<1..1106)
                     /locus_tag="VIV63_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY6124549.1"
                     /translation="MKVVKVSTSHIQAVTGTTFVSTLSSLLFGYCTTVIAGVVGAIDY
                     NFIAPRGMHETAANALLGLTVCAALGGTILGALVARRTAELLGRKKPMILASILFIVS
                     AFGSAFPETGISAIGAMGPDAIWPFIFYRVVGGVAVGLASVIAPMYVAEFAPRAVRGQ
                     LGAYQQIAIAGGIALVLFVNWGIALQGDDAWVLTTGWRYMMVSMAVPALAFFWLSFSV
                     PESPSWLVKHGRVEAARRMLSRSADSDEVEAMLADLAKDHSVHVKPAPLLAFGARVVF
                     IGVALSVLQQLMGLNAISYYGPEILQRMGFHMDDAFLGVLIARSLNLLATMMVVLIVD
                     RVGRKPLLIFGALTMGLSMMALGALFENDNTGAYG"
     gene            complement(1114..>2137)
                     /locus_tag="VIV63_07850"
     CDS             complement(1114..>2137)
                     /locus_tag="VIV63_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124550.1"
                     /translation="IDESRSGNFELETTVLTADEKRVDLRWVASCEQSENTGQHKVLR
                     GMMLDVTDQRRLETELAQAQKLESVGRLAAGVAHEINTPVQFVSDSVHFVREAMDDLS
                     GIVDKYRELRTATENGTDVKAAAKAVEEAEDDADLDYILENAPVALDRARDGLGRVAA
                     IVRSMKEFAHPDRKEMAQSDLNQAIASTLVIASNEYKYVAEVETRFAELPLVNCYAGE
                     INQVVLNLIVNAAHAIGDVVKGTAAKGKITVTTRVLDASVEIAIGDTGKGIPVEVRGR
                     IFDPFFTTKEVGKGTGQGLAIARTVVVDKHGGTLHFETEVGRGTTFFIRLPIDGPTKA
                     TATAAA"
BASE COUNT          376 a          697 c          692 g          372 t
ORIGIN      
        1 ccgtaggcgc cggtgttgtc gttctcgaac agcgcgccca gcgccatcat cgacaggccc
       61 atggtcaacg cgccgaagat cagcagcggt ttgcggccca cgcgatcgac gatgaggacg
      121 accatcatgg ttgcgagcag attgagcgac cgcgcgatca gcacgcccag gaaggcgtcg
      181 tccatgtgaa agcccatgcg ctgcaggatt tccggaccgt aatacgagat ggcattgagc
      241 cccatgagct gctgcagcac gctcagcgcg acgccgatga acaccacacg cgcgccgaat
      301 gccagcagcg gcgccggctt cacgtgcacg ctgtggtcct tggcgagatc cgcgagcatc
      361 gcctcgacct cgtcggaatc ggccgagcgg gaaagcatgc ggcgcgcggc ctcgacacgg
      421 ccgtgcttca ccagccagct cggggattcc ggcacgctga aactgagcca gaagaacgcg
      481 agcgccggca ccgccatcga caccatcatg taacgccagc cggtcgtcag tacccaggcg
      541 tcatcaccct gcagggcgat gccccagttc acgaacagca ccagggcgat gccgccggca
      601 atcgcgatct gctggtaggc gcctaactgg ccgcgcaccg cgcgcggcgc gaactcggcc
      661 acgtacatcg gagcgatgac cgacgccagg cccacggcca cgccgccgac gacacggtag
      721 aagatgaagg gccagattgc atccggcccc atggcgccga tggcggagat gcccgtttcc
      781 gggaatgccg atccgaacgc cgagacgatg aacaggatcg aggccaggat catcggcttc
      841 tttcggccta acaactccgc ggtcctgcgg gcgaccagcg cgcccaggat ggtgccgccg
      901 agagccgcgc agaccgtgag tccgagcaac gcgttggcgg cggtttcatg catgccgcgt
      961 ggcgcgatga agttgtagtc gatggcgccg accacgccgg cgatgaccgt cgtgcagtag
     1021 ccgaacaaca gcgaactcag cgtcgatacg aatgtcgtgc cggtgacggc ctggatgtga
     1081 ctggtggaaa ccttgacgac tttcatgatg tcctcaggcc gcggcagtcg ccgtcgcctt
     1141 cgtcgggccg tcgatcggca accggatgaa gaacgtggtg ccccgcccca cttcggtttc
     1201 gaaatgcagt gtgccgccgt gtttgtcgac caccacggtg cgcgcgatcg cgagtccctg
     1261 gcccgtgccc ttgccgactt ccttggtggt gaagaacgga tcgaagatgc ggccacgaac
     1321 ctcgacggga atgcccttgc ccgtgtcgcc gatggcgatt tcgaccgatg cgtcgagcac
     1381 gcgcgtggtc acggtgatct tgcccttggc cgccgtgcct ttgacgacat cgccaatggc
     1441 atgcgccgca ttcacgatga ggttcaacac cacctggttg atctcgcccg cataacaatt
     1501 cacgagcggc agctcggcga agcgcgtctc gacttcggcg acgtacttgt attcgttgga
     1561 ggcgatgacc agcgtgctgg cgatcgcctg gttgaggtcg gactgcgcca tctccttgcg
     1621 atcgggatgc gcgaattcct tcatggaacg cacgatggcg gcgacgcggc cgagaccgtc
     1681 gcgtgcgcga tcgagcgcga ccggcgcgtt ctcgaggatg tagtcgagat cggcgtcgtc
     1741 ctcggcctcc tcgacggcct tggcggcggc cttcacgtcg gtgccgttct ccgtggcggt
     1801 gcgcagctcg cggtacttgt cgacgatgcc ggacaggtca tccatggctt cgcgcacgaa
     1861 gtgcacgctg tcggaaacga actgcaccgg ggtattgatt tcgtgggcca cgcccgcggc
     1921 caggcggccc acgctctcga gcttctgcgc ctgcgcgagt tcggtttcga ggcggcgctg
     1981 atcggtgacg tcgagcatca tgccgcgcag caccttgtgc tggccggtgt tctcgctctg
     2041 ttcgcaactg gccacccagc gcagatccac gcgtttttcg tcggccgtaa gcacggtggt
     2101 ctcgagctcg aaattgccgg acctgctctc gtcgatg
//
LOCUS       DATDEU010000329         2276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1082120, whole genome shotgun sequence.
ACCESSION   DATDEU010000329 DATDEU010000000
VERSION     DATDEU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082120"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="ppnN"
                     /locus_tag="VIV63_07855"
     CDS             <1..868
                     /gene="ppnN"
                     /locus_tag="VIV63_07855"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF038390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HEY6124551.1"
                     /translation="TKKVGYELGLRGLDVCTGCGPGAMKGPMKGATIGHAKQRIVGGR
                     YIGLTEPGIIAAEPPNPIVNQLVIMPDIEKRLEAFVRLAHGVVVFPGGVGTAEEILYL
                     LGILLEPANAGQPLKVVLTGPKSTSDYFIQIRRFIEGTLGTKAAELLHVIVDDPAEVA
                     RYMVSTMAQARYFRRNNSDSYNYNWLLKVPAPFQHPFEVSHESMRALRLYRDQPAHEL
                     AADLRRAFSGIVTGNVKENGVRAIEKLGPYELTGDASVMKLLDELLTAFVAQKRMKLP
                     GSAYRPCYRLVA"
     gene            1071..1292
                     /locus_tag="VIV63_07860"
     CDS             1071..1292
                     /locus_tag="VIV63_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124552.1"
                     /translation="MSASDRNRGNLANGTPPPVGTDAYAAWLERMQRTRGRHAAITKN
                     LYTWSSYKHWADKVKGSWEEEPVPAPTKK"
     gene            complement(1298..>2276)
                     /locus_tag="VIV63_07865"
     CDS             complement(1298..>2276)
                     /locus_tag="VIV63_07865"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HEY6124553.1"
                     /translation="VARGSRKYYQEGLFPNVTTATDPGLIPWRHWFADAKRIPVDPDE
                     NGVGRFEGAYYMPIGFYRPKRDSFMRHLDAPIGEVNAEAWLRALYRALPPLRASLPAH
                     RTVIGLPGETLDFRIASEWPAKIMAVRWYIDGVEAEGARDAWRYGLQADGLTHEVRVS
                     IEDRTGLIRDPDANEHKGSAVWTVSDDHRPMRMAKASQGHRISGWVRMRVDSTGHTVV
                     GVTQTEARSLHRLGTAADSGFESTLFDAEGALLSRQRIADPRMVRGPLPGRGMAAAGH
                     GIATLESGYYLIEIPEGADARKLRIRAVTGAEKTEPMTEGSPTELWLDL"
BASE COUNT          439 a          752 c          696 g          389 t
ORIGIN      
        1 caccaaaaag gtgggctacg agctcggcct tcgcggcctg gatgtgtgca cgggctgcgg
       61 tccgggcgcg atgaagggac ccatgaaggg cgccaccatc ggccacgcca agcagcgcat
      121 cgtcggtggc cgctatatcg gcctcaccga gcccggcatc atcgccgcgg agccgccgaa
      181 cccgatcgtg aaccagctcg tgatcatgcc ggacatcgaa aagcgcctcg aagccttcgt
      241 acggctcgcg cacggcgtcg tggtgttccc gggtggcgtg ggtaccgccg aggagatcct
      301 gtatctgctc ggcatcctgc tcgagcccgc caacgcgggg cagccgctca aagtcgtgct
      361 cacgggtccg aaatccacgt ccgactactt catccaaatc cggcgtttca tcgaaggaac
      421 cctggggaca aaggccgccg agctgctcca cgtgatcgtg gacgacccgg ccgaagtagc
      481 gcgctacatg gtttcgacca tggcgcaggc gcgctatttc cgcaggaaca acagcgactc
      541 gtacaactac aactggctgc tcaaggtccc cgcgccattc cagcatccgt tcgaggtgtc
      601 gcacgaatcc atgcgcgcgc tgcgcctcta tcgcgaccag ccggcgcatg agctggccgc
      661 ggatctgcgg cgcgcgttct ccggcatcgt caccggcaac gtgaaggaaa acggcgtgcg
      721 cgccatcgag aagctaggcc cgtacgagct caccggcgac gcgtcggtga tgaaactact
      781 cgatgaattg ttaaccgcgt tcgtcgcgca gaagcgaatg aaacttccgg gaagcgctta
      841 ccggccgtgc taccggctcg tcgcgtgacg ccgctcgtct cgcaaacaac acaataacca
      901 actacaacgt taaatcgccg cgtgcgtttc gcgcgtcggc gatacataac gaaaaaatct
      961 gtgcgaacca gcgcgcatcg cccgcaagct gtcgtgggcg cgtgaatcag ttcctgtcgt
     1021 tttggtgcga cgaaatcgta ggcttacgtc aaccgaaagg gagtaagccc atgagtgcct
     1081 ctgacagaaa ccggggaaat ttagcgaacg gcacgccgcc tccggtgggc accgacgcgt
     1141 atgccgcctg gctcgagcgc atgcagcgca cgcgcgggcg tcatgctgcg atcaccaaga
     1201 atctctacac ctggtctagc tacaagcatt gggcagacaa ggtgaagggt tcgtgggaag
     1261 aagagccggt cccggctccg accaagaagt agacgctcta gagatcgagc cacagttccg
     1321 tcggcgatcc ctcggtcatc ggctccgtct tttccgcccc cgtcaccgca cgaatccgca
     1381 acttgcgggc gtcggcacct tccggaatct cgatcaggta atagccactt tccagtgtgg
     1441 caatgccatg cccggccgca gccatgcccc ggcccggcaa cggcccgcgg accatgcgcg
     1501 gatcagcaat gcgctgccgg gataacaagg ctccttctgc atcgaagagc gtggactcga
     1561 acccggagtc cgcggcagtg cccaggcgat ggaggctgcg cgcttcggtc tgggtgacgc
     1621 ccacgacggt atgccccgtc gaatcaacgc gcatacgaac ccatccgctg atgcggtgcc
     1681 cttgcgacgc cttggccatc ctcatcggac gatggtcgtc cgataccgtc cagacggcgc
     1741 tgcccttgtg ttcgttggca tccgggtcgc ggatgagccc ggtgcgatct tcaatcgaca
     1801 cccgcacctc gtgagtcagg ccgtcggcct gcaacccgta gcgccacgcg tcgcgcgcgc
     1861 cttcggcttc cactccgtcg atgtaccagc gcaccgccat gatcttcgcc ggccactcgc
     1921 tggctatcct gaagtccagc gtctcgcccg gaaggccaat caccgtccga tgtgccggta
     1981 acgaagcgcg caacggaggc agcgcacgat agagcgcacg cagccaggct tccgcgttca
     2041 cctcgccgat gggcgcgtcc aggtggcgca tgaagctgtc gcgcttgggt cgatagaacc
     2101 cgatgggcat gtagtacgca ccttcgaaac gcccgacgcc gttctcatcc ggatcgaccg
     2161 gaatgcgctt cgcatcggca aaccagtggc gccaggggat gaggccggga tcggtggcgg
     2221 tggtgacgtt agggaagagc ccttcctggt agtacttccg cgatccgcgc gcgacg
//
LOCUS       DATDEU010000330         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1810588, whole genome shotgun sequence.
ACCESSION   DATDEU010000330 DATDEU010000000
VERSION     DATDEU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810588"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VIV63_07870"
     CDS             <1..187
                     /locus_tag="VIV63_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124554.1"
                     /translation="DAQAAQAAGMYSLIAGFGYIGDDDRADEWFQHGWLNTPLDLLAW
                     LDNPRTLPRAKRHNGSA"
     gene            184..1488
                     /gene="rmuC"
                     /locus_tag="VIV63_07875"
     CDS             184..1488
                     /gene="rmuC"
                     /locus_tag="VIV63_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY6124555.1"
                     /translation="MSSYWFIGAIVAIAAAVIGYLVGTLRAGRRATELGNALAEARAR
                     VASEAEQLARTEELLKQADAQVRAAVESASRAALNANSETFLKLAREVFGRDQEVANA
                     TLQARELAIAQLVEPIKAALAKQEEQTQALERERRETFGVMRTQIESMSSGQALLQRE
                     TRNLVTALRRPEVRGRWGELTLRRVVELSGLSEHCDFTEQEHLAATGERGALRPDLLV
                     HMPEARDLVVDAKTPLDGYLDAIEAQDDITRSAALARHAMQVEQRVRELGQKSYWEQF
                     EQSPEFAVLFLPGDQFLSAALVERPDLLENALKQSIIIATPSTLMALLKVVAYGWRQA
                     AASENAAEIRELGAELHKRLTTFVGHLQKVGRSLDNALGAYNSAVGSFERNVTPQARR
                     FTELGATPDTLPTVDQIEQVVRLPAGNPALESDSTDKTRTPS"
     gene            1485..2243
                     /locus_tag="VIV63_07880"
     CDS             1485..2243
                     /locus_tag="VIV63_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HEY6124556.1"
                     /translation="MTTFDPRRHQPSPGELRGRVIMVTGAGSGLGRAVALSLAGAGAE
                     VILLGRTVRKLEAVHAEIAAAGGPEASIVPLDLERALAADYEAVAAAVEKRYGRLDGL
                     LHNAALLGALTPIEQYEVPMFMRVMHVNLTAAFVLTQHLLPLLRASKDAAVLFTSSGV
                     GTRGRAYWGAYSVSKFAVEGLTQVLAHELEGTSTVRVNIIDPGKVRTPMRRQAYPSEA
                     AESLPTPESLTGPYIALLGPASAGITGQRFNAQG"
BASE COUNT          413 a          779 c          734 g          351 t
ORIGIN      
        1 cgatgcgcaa gccgcgcagg ccgccggcat gtattcattg atcgcgggtt tcggatacat
       61 cggcgacgac gaccgggccg atgagtggtt ccagcacggc tggctcaata cgccgctcga
      121 cctgctcgcc tggctcgaca atccccgcac gctcccgcgc gccaagcggc acaacggaag
      181 cgcatgagtt cctactggtt catcggggcc atcgtcgcga tcgcggccgc cgtgatcggc
      241 tatctggtcg gtaccctgcg cgcaggtcgg cgcgccaccg aactcgggaa cgcgctggct
      301 gaagcgcgcg ccagggttgc ttccgaagca gaacaactcg cgcgcaccga agaactgctc
      361 aagcaggccg acgcacaggt gcgcgccgcg gtcgagagtg cgtcgcgcgc agcgctgaac
      421 gccaacagcg aaactttcct caagcttgcg cgcgaagtgt tcgggcggga ccaggaagtg
      481 gccaatgcca cgttgcaggc acgcgagctc gccatcgcgc agctcgtcga gccgatcaag
      541 gccgcactcg ccaagcagga agaacagacc caggcgctgg agcgggaacg gcgcgaaacc
      601 ttcggcgtca tgcgcacgca gatcgaatcc atgtcgtcgg gccaggcgct gctgcagcgc
      661 gaaacccgca acctggtcac cgcgctgcgc cgcccggaag tccgcggccg ttggggcgaa
      721 ctcacgctgc ggcgcgtggt cgagctctct ggcctttccg agcactgcga tttcaccgag
      781 caggagcacc tggccgccac cggcgaacgc ggcgccttgc gcccggatct tctggtccac
      841 atgcccgagg cacgcgatct cgtggtcgac gccaaaacgc cgctcgacgg ctatctcgat
      901 gcgatcgaag cgcaggacga catcacgcgc agcgccgcac ttgcgcgcca cgccatgcag
      961 gtggagcaac gcgtgcgcga actcgggcag aagagttact gggaacagtt cgagcaaagt
     1021 ccggaattcg ccgtgttgtt cctgccggga gatcaattcc tgtccgccgc gctcgtcgaa
     1081 cggcccgatc tgctcgagaa cgcactcaag caaagcatca tcatcgcgac gccctccacg
     1141 ctcatggcgc tgctcaaggt cgtggcctat ggctggcgcc aggccgcggc ttcggaaaac
     1201 gccgcggaaa tccgcgagct cggcgcagag ctgcacaaac gactcaccac tttcgtgggg
     1261 catttgcaga aggtgggccg ctcgctcgac aacgcactcg gcgcgtacaa ctcggcggtg
     1321 ggttcgttcg agcgcaacgt caccccgcag gcgcggcgat tcaccgaact cggcgcgacg
     1381 ccggacacgc tgcccaccgt cgatcagata gagcaggtag ttcggttgcc tgcgggcaat
     1441 cccgccctcg aatccgacag caccgacaag acacgcacac cttcatgact acttttgatc
     1501 cgcgccggca tcagccgtcg cctggcgaat tgcgcggccg cgtcatcatg gtcacgggtg
     1561 cgggctcagg gctgggccgt gccgtggcgc tctcgctcgc gggagccggc gccgaagtca
     1621 tcctgctcgg ccggacggtg cgcaaactcg aagccgtgca tgcggagatc gccgcggcgg
     1681 gcggccccga agccagcatc gtgccgctcg acctcgaacg tgcgctggcg gcggactatg
     1741 aagccgtggc cgcggccgtc gaaaaacgtt acggccgcct cgacggcctc ctgcacaacg
     1801 ccgcattgtt aggcgcgctc acgcccatcg agcagtacga agtgccgatg ttcatgcgcg
     1861 tcatgcacgt caacctcacc gccgcgttcg tgctcaccca acatctgctc ccgctgctgc
     1921 gagcttcgaa agacgccgcc gtgctgttca cgtcgagcgg cgtcggcacg cgaggccgcg
     1981 cctactgggg cgcgtactcc gtttcgaagt tcgcggtgga aggtctcacg caggtattgg
     2041 cgcatgagct cgaaggcacg agcacggtgc gcgtgaacat catcgatccg ggcaaggtac
     2101 gcacgcccat gcggcggcag gcctatccat ccgaagcggc cgaatcgctg ccgactccgg
     2161 aatcgctgac ggggccgtat atcgcgttgt taggccccgc gagcgccggg atcacgggcc
     2221 agagattcaa cgcgcaagga tgatcgcgcg gggctgaccg cccggcgctt caatctt
//
LOCUS       DATDEU010000331         5696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_499068, whole genome shotgun sequence.
ACCESSION   DATDEU010000331 DATDEU010000000
VERSION     DATDEU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499068"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VIV63_07885"
     CDS             <1..666
                     /locus_tag="VIV63_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6124557.1"
                     /translation="NRVRSFVFTSTTSTFGDALTPPAGAPAVWITEDVVPIPKNIYGA
                     TKVAAEDLCQLFHRNQKLPCLILRTSRFFPEDDDVPEKRAAFEEANLKTNELLYRRVD
                     VEDVVSAHVLAMERAPALGFGKFIITATTPFARDDAPELGVDAAAVLRRRVPGFEAIY
                     ATQGWKMLPALDRVYDNARARTVLGWAPKYDFARALHSLSAGIDPRSELARAIGSKGY
                     HPT"
     gene            663..1343
                     /locus_tag="VIV63_07890"
     CDS             663..1343
                     /locus_tag="VIV63_07890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6124558.1"
                     /translation="MTQLLDNIMWNCLSGPHAKFATGSGAVRRYAPGFSPIVGCEDPQ
                     QPDFDTLQKYCEPGDSFYFDIWSGPAPAGWHIDKEAHMFKMVWEAPMPAEDPAPDAMA
                     LRPEHYEQAVSLAQLTNPGPFGIRTPELGEYFGFFEGDRLVAMAGERLCAGDLQEVSG
                     ICTHPDFQGRGLARKLTLKLVRRQMQRARISFLHVVSHNAPARGLYEKMGYRNYLETV
                     VRVVTRVI"
     gene            1350..2015
                     /locus_tag="VIV63_07895"
     CDS             1350..2015
                     /locus_tag="VIV63_07895"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY6124559.1"
                     /translation="MLIIPLGRKGSDGIPLVTLFVCVACIIVHVFANAPADRLALAFH
                     PDQLDPLKMFTSVFTHADILHLLGNLFFFYCFARTIEARITTTGFILAFVVFVLVTSL
                     SYAATAREPIPTVGLSGVVWGYMGMFLLRYPKDRIECWVLFLNKIEVPASIFILAFLA
                     LDIAAYRHTEIAPVNYIAHFSGFAAGALFKLAFWNTFTTEKPEPRKKTPFPRLVHGRP
                     ARR"
     gene            2073..2414
                     /locus_tag="VIV63_07900"
     CDS             2073..2414
                     /locus_tag="VIV63_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124560.1"
                     /translation="MDKKRFIGLAGLAVPAAMLGCSTSKPTPSVQTEAIEWRSTQLIP
                     PPPAMEPRGQFCLLGTTCLAMDPRPFEPCLLSTKHCGDKAREPMEVAPPREVPDSGAE
                     ASRIITVIAPQ"
     gene            2424..2804
                     /locus_tag="VIV63_07905"
     CDS             2424..2804
                     /locus_tag="VIV63_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124561.1"
                     /translation="MRTLSLVLMALGVSALTSAETTIPSREAELTAIPTADHPYAGFW
                     KSNDCTDRWGLAISPSSSPGLYSVSFCGPGGCFTPGTYRPETTLTNDEKYWVIDVNTI
                     EVGGCGTFEGSFTRYVRCPGREVP"
     gene            2940..3371
                     /locus_tag="VIV63_07910"
     CDS             2940..3371
                     /locus_tag="VIV63_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6124562.1"
                     /translation="MKLNSATVKRLREAKSWTQEHLASAAGVSLRTVQRLEADGSASA
                     ESRLAIAAALGVPVDSIHLAPDSVESASAAELRRVAVGTRWGYTGVAIGTLASAAGIV
                     SGSASPEQAATALGILGAFAGITAGAIGVLAQRAVRLASDS"
     gene            3377..3748
                     /locus_tag="VIV63_07915"
     CDS             3377..3748
                     /locus_tag="VIV63_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6124563.1"
                     /translation="MTREEARAFAATWATAWNDREIERVLGFFAEDVEFVSPTALAVT
                     GTATVRGKRALRAYWNSALSGIHSLRFTVDRVMWDASTRELAIIYVSHIDGRAKRVSE
                     NLTFGEGGLVVSAEVFHGVPA"
     gene            3745..4068
                     /locus_tag="VIV63_07920"
     CDS             3745..4068
                     /locus_tag="VIV63_07920"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HEY6124564.1"
                     /translation="MKMFYLLCAIAGAVIPYSQFVPWVAEHGLDVRLLAVELFSTRIG
                     AFFGLDVLVSAVALLAFIAWEGRRRGMKMLWLPAAATCLIGVSCGLPLFLFMREQQPI
                     LAEVK"
     gene            complement(4166..5590)
                     /locus_tag="VIV63_07925"
     CDS             complement(4166..5590)
                     /locus_tag="VIV63_07925"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEY6124565.1"
                     /translation="MNTKLLVNGKLVKGKGEELGVLDPATGKVIAQVAEASEEQIAAA
                     VKAAAKAFPAWAATVPKDRSTLLLKLADRIEADALEFAKLESQNCGKPLNAALNDEIP
                     AIADVFRFFAGAARSMHGALAGEYLPGFTSMIRRDPIGVVASIAPWNYPLMMAAWKMG
                     PALAAGNTVVLKPSEQTPLTALKLATLCAELFPPGVVNIICGRGNTVGAPLVAQPEVA
                     MISLTGSIASGEKVLAAAVNGTKRTHLELGGKAPVIVFDDADLESVVGGVRTFGFYNA
                     GQDCTAACRIYAGPKIYDKLVADLSSAASSIKMGTQEEEGVEMGPLITSRQRSRVAGF
                     VQRATEKRHIEVTAGGKARTGSGFFYEPTVIAGAKQTDEIVQKEVFGPVVSVTRFKDA
                     DQAIEWANDSDYGLASSVWTKDVGKAMKVAARLQYGCTWVNTHFMLVSEMPHGGMKRS
                     GYGKDLSVYALEDYTAVRHVMVKF"
BASE COUNT          990 a         1830 c         1788 g         1088 t
ORIGIN      
        1 aaccgggtgc gctcgttcgt gttcaccagc accacaagca ctttcggcga cgcgctcacg
       61 ccgccggccg gcgcgcctgc cgtgtggatc acggaagacg tggtgccgat cccgaagaat
      121 atttacggcg ccaccaaggt cgcggctgaa gacctgtgcc agctcttcca ccggaaccag
      181 aagttgccct gcctcatctt gcgcacgtcg cggttctttc ccgaagacga cgacgtgccg
      241 gagaaacgag cagcgttcga agaggcgaat ctcaagacga acgaactcct gtatcgccgc
      301 gtggacgtgg aagacgtggt cagcgcgcat gtgctcgcca tggagcgggc gccggcgctg
      361 ggtttcggaa aattcatcat caccgcgacg acgccgttcg cccgcgacga cgcacctgaa
      421 ctcggtgtgg atgcggcggc cgtactgcga aggcgagttc ccggattcga ggctatctac
      481 gccacgcagg gatggaagat gcttcccgcg ctcgaccgcg tgtatgacaa cgcgcgtgcg
      541 cgaaccgtgc tcggctgggc gccgaaatac gacttcgccc gcgcgctgca cagcctgtcg
      601 gccggcatcg atccgcgcag cgaactcgcg cgtgccatcg gatcgaaggg ctaccacccg
      661 acgtgacaca gttgctcgac aacatcatgt ggaattgtct ttcaggtccg cacgcgaaat
      721 tcgcgaccgg atccggcgcc gtcaggcgct acgcgcccgg tttctcgccc atcgtgggtt
      781 gcgaggatcc gcagcagcct gacttcgaca ccttgcagaa atactgcgag cccggcgaca
      841 gcttctattt cgatatctgg tccggcccgg cgccggccgg ctggcacatc gacaaggaag
      901 cgcacatgtt caagatggtc tgggaagcac ccatgccggc cgaagatccg gcgccggacg
      961 ccatggcact tcgtcccgaa cattacgagc aggccgtgag cctggcgcaa ctcaccaacc
     1021 cgggtccttt cggaattcgc acgccggaac tcggggagta cttcggcttc ttcgagggtg
     1081 accggctcgt tgcgatggcc ggcgaacgcc tgtgtgcggg cgatctgcag gaagtcagcg
     1141 gcatctgcac gcacccggac ttccagggcc gcgggctcgc gcgcaagctg acgctgaagc
     1201 tcgtgcgccg gcagatgcag cgcgccagga tttcattcct tcatgtcgtg agtcacaacg
     1261 cgccggcgcg tggcctgtac gaaaagatgg gctatcgaaa ctatctcgag accgtcgtcc
     1321 gcgtcgtgac gcgtgttata taggcgctca tgctgatcat ccctctcggc aggaaaggtt
     1381 cggacggcat tcccctggtc acgctgttcg tgtgcgtggc ctgcatcatc gtccacgtgt
     1441 tcgccaacgc gccggccgac cggttggcac tcgcgtttca ccccgatcaa ctcgacccgc
     1501 tcaagatgtt cacgtccgtg ttcacacacg cggacatcct gcacctgctg ggcaacctgt
     1561 tcttcttcta ctgtttcgcg cgcacgatcg aagcgcgcat caccacgacg gggttcatcc
     1621 tggctttcgt ggtgttcgtg ctggtcacca gcctctccta cgccgccacg gcgcgcgagc
     1681 ccattcccac cgtcggcttg tcgggcgtcg tgtggggata catgggcatg tttctgttgc
     1741 gctatccgaa agaccgtatc gaatgctggg tgttgttcct gaacaagata gaagtgccgg
     1801 cttcgatctt catacttgcg ttcctcgcgc tcgacatcgc cgcctatcgg cacactgaaa
     1861 tcgctccggt gaactacatc gcgcacttct cgggttttgc tgcgggcgcg ttgttcaaac
     1921 tggctttctg gaacacgttc accaccgaaa agccggagcc caggaagaag acgccgttcc
     1981 cgcgcctggt gcacggccgg ccggcgcgaa gataacgagt ggattgaacc ccggcgggcc
     2041 acgtgggtcg tttgtgtatc ggagggatac acatggacaa gaagaggttc atcgggcttg
     2101 ccggtctggc agtgcccgcc gcaatgctgg gatgcagcac atcgaagccg actccttcgg
     2161 tgcagacgga agcgatcgag tggcgcagta ctcaactcat cccgcctcct cccgccatgg
     2221 aaccgcgcgg tcagttttgt ttgctaggca cgacctgcct ggcgatggat ccccgtccgt
     2281 tcgagccctg cctgctttcg accaagcact gtggcgacaa ggcgagggaa ccgatggaag
     2341 tcgcgccgcc gcgcgaggtt ccagattccg gtgctgaagc gagccggatc atcacggtca
     2401 tcgcgccgca gtagggaatc ttcatgcgga cactttcact cgtgctcatg gccctgggtg
     2461 tctcggcgct gacgtcggcg gagaccacga tcccctctcg cgaagcggaa ctcaccgcga
     2521 taccgaccgc ggaccatcca tacgccggat tctggaagtc gaatgactgc accgaccggt
     2581 ggggccttgc gatctcgcct tccagcagcc cgggtttgta ttcggtttcc ttctgcggcc
     2641 cgggtggatg ttttacccca ggcacctacc ggcccgagac cacgctcacc aacgacgaaa
     2701 agtattgggt catcgacgtg aacacgatcg aggtgggggg ctgcgggaca ttcgagggct
     2761 cattcacgcg ctatgttcgc tgcccgggac gcgaagtccc ctgacaacct gactgcgccc
     2821 tgacggctct ctgacgcgaa cctggcggcc ggttgtcggg tgatgtcgta tgctgttgcc
     2881 gccgcaactg ccacgctccc gggccccgca aacacccgga gagaaaaatg gagagcgaag
     2941 tgaaactgaa ttccgccacc gtcaaacggc ttcgcgaggc gaagtcatgg acccaggaac
     3001 atctggctag cgcagccggc gtcagcctgc gcaccgttca gcgcctggaa gcggacggat
     3061 cggcgtccgc ggaatcgaga ctcgccattg ccgccgctct cggcgtcccc gtggacagta
     3121 tccacctcgc gccggattcg gtcgaaagtg ccagcgctgc tgaattgcgt cgcgtcgcgg
     3181 tcggcacgcg ctggggctac accggcgttg ccattggcac gctggcatcc gccgcgggca
     3241 tcgtgagtgg gagcgccagt cctgagcagg cagccactgc cttgggaatt ctcggagcgt
     3301 ttgccggcat caccgcgggc gccatcggcg tgctggcgca gcgcgcggtc agactcgcaa
     3361 gcgactcctg acaatcttga cccgcgaaga agcacgagca ttcgccgcca cgtgggcgac
     3421 tgcgtggaac gaccgggaga tcgagcgcgt gctcggtttc ttcgcggaag acgtcgagtt
     3481 tgtgagcccc accgcgctcg ccgtgaccgg cacggcgacg gtgcgaggta agcgcgcgct
     3541 tcgggcctat tggaattccg cgctgagcgg aatccactcg ctgcgattca ccgtcgaccg
     3601 cgtgatgtgg gacgcttcca cgcgcgagct cgcgatcatc tatgtgtccc acatcgatgg
     3661 ccgagcgaaa cgcgtgtcag aaaatttgac gttcggcgag ggtggtctcg tggtcagcgc
     3721 cgaagtgttc cacggagtcc ccgcatgaag atgttttacc tgctctgcgc catcgccggc
     3781 gcagtcattc cctacagtca gttcgtgcca tgggtcgcgg agcacggtct cgacgtgaga
     3841 cttctggcgg tcgaattgtt ctcgacacgc atcggcgcat tcttcggact cgatgtgctg
     3901 gtgtcggccg ttgccttgct ggcgttcatc gcctgggaag gacgacgacg cggcatgaaa
     3961 atgctttggc tgccggcagc cgccacctgc ctgatcggcg tgtcttgtgg actgccactg
     4021 ttcctgttca tgcgtgaaca gcaacccatt ctcgcggagg tgaaatagca tggctacggg
     4081 gaccgtcaac ctcggctggc aggaatcact ggcgcagctc gcgcatctcg tcgagcccga
     4141 aattccggac tgacgaaatc ccggactaga acttcaccat gacgtgtcgc acggcggtgt
     4201 agtcctcgag cgcgtagacg ctcaggtcct tgccatagcc cgaacgcttc atgccgccat
     4261 gcggcatctc gctcacgagc atgaagtggg tgttcaccca ggtgcatccg tactgcaggc
     4321 gcgcggccac tttcatggcc ttgcccacgt ccttggtcca cacggaagac gcgaggccgt
     4381 agtcggaatc gttcgcccac tcgatggcct ggtccgcgtc cttgaagcgc gtgacgctga
     4441 ctaccgggcc gaagacttcc ttctgcacga tctcgtcggt ctgtttggcg cccgcgataa
     4501 cggtgggctc gtagaagaat ccgctgcccg tgcgcgcctt gccgcccgcg gtcacctcga
     4561 tgtggcgctt ctcggtggcg cgctgcacga aacctgcgac tcgcgaacgc tgccgggagg
     4621 tgatgagggg ccccatctcg acgccctctt cctcctgcgt gcccatcttg atgctgctgg
     4681 cggcgctcga cagatccgcc acgagcttgt cgtagatctt gggaccggca tagatgcggc
     4741 aggcggcggt gcagtcctgg cccgcgttgt agaagccgaa ggtacgaacg ccgccgacca
     4801 cggactcgag atccgcgtcg tcgaacacga tgacgggtgc cttgccaccc agttcgagat
     4861 gcgtgcgttt cgtgccgttc accgcggccg cgaggacttt ctcgccgctg gcgatggagc
     4921 cggtgagcga gatcatcgcc acttccggct gcgcgacgag cggcgcgccg acggtgttgc
     4981 cgcgaccgca gatgatgttg acgacacccg gcgggaacag ctccgcgcac agcgtggcga
     5041 gtttgagtgc cgtgagcggc gtctgctcgg aaggcttgag caccacggtg ttgcccgcgg
     5101 cgagcgccgg gcccatcttc caggccgcca tcatgagtgg atagttccag ggcgcgatgg
     5161 acgcgaccac gccgatgggg tcgcggcgaa tcatgctggt gaagccgggc aggtattcgc
     5221 ctgccagcgc accatgcatg gagcgtgcgg cgcccgcgaa gaagcggaac acgtcggcga
     5281 tagccgggat ctcgtcattc aatgccgcgt tcaatggctt gccgcagttc tgcgactcga
     5341 gtttcgcgaa ttccagggcg tccgcctcga tgcgatccgc gagtttcagc agcaacgtgc
     5401 tgcgatcttt cggcaccgtc gcggcccagg cgggaaatgc tttcgccgcc gctttcacgg
     5461 ccgcggcgat ctgttcctcc gatgcttcgg ccacctgcgc gatcaccttg cccgtggccg
     5521 gatcgagcac gcccagctct tcacccttgc ctttgaccag cttgccgtta acgagcagtt
     5581 tcgtgttcat cgtcgggcct cgtgaggtag taagcgagca caatcggaat cagcgtcacg
     5641 gcgatgacga tgaccgccac cacgttggtc accggccggt cgcgcggccg gaacag
//
LOCUS       DATDEU010000332         2628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_790403, whole genome shotgun sequence.
ACCESSION   DATDEU010000332 DATDEU010000000
VERSION     DATDEU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790403"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VIV63_07930"
     CDS             <1..223
                     /locus_tag="VIV63_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124566.1"
                     /translation="LAALKPPVFWGRGTNDDVIPEFLVDHTTEWLPGHAELSGRVYPG
                     LTHSVSEQELDDVRVFLDKQLEAMPTLGG"
     gene            507..1211
                     /locus_tag="VIV63_07935"
     CDS             507..1211
                     /locus_tag="VIV63_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6124567.1"
                     /translation="MPAHTLTPSIILDRVSFAWPDGSPALTGLSGAFGDGRTGLVGRN
                     GSGKSTLLRTIYGDHAVASGGSIVRAGIVPGVALEKFKLRTAYVAPHLQTWHMPKMPV
                     MEVVASGRYASIGLNDSITARDRKHAERALRRFGMFALRKRTLAEMSYGQARRILFAR
                     AWAREPALALLDEPFAGLDRATRADLTRRLNEWLAEGGSCVIATHHREEWPAHTTHVL
                     ELKAGRAIASREVGEA"
     gene            1208..2212
                     /locus_tag="VIV63_07940"
     CDS             1208..2212
                     /locus_tag="VIV63_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY6124568.1"
                     /translation="MKKLGLLAVVAVIAAIAFVMFRSGESAPERIVVKLVPGPPQPKV
                     TPAEAGIDMAAIQAAVDYAGKRNTSALLIGQGGHLVFEKYWGDGTLDTPLSASQFGRV
                     LVPLLVGSAMNDRLIISLDEPVSNYLPEYADTPAGKATLRDHLGQRVEGSMPGSWEML
                     ALVLERVTKQSFDVLVTERLWKPLEAGEFSMERNGDNEVRKGAAKAGCCVVARIGDWM
                     RIGEMLANDGVFEGNQFAPPRYVTLMQKPTHNLSRLGLGMNVGGDFAADVAWLADGTQ
                     RLWVVPSLRLVILRVGDAPDEADGWDEVMIPDSIIRGTSGWQPRSVGEGLDPKKFAPH
                     "
     gene            complement(2294..>2628)
                     /locus_tag="VIV63_07945"
     CDS             complement(2294..>2628)
                     /locus_tag="VIV63_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY6124569.1"
                     /translation="AQLGAAGFFVFTLSHGFTSCLTESRMFCPALGIPEDPVSGNAHG
                     LLGAYLAEQGLLPHAGPMAQFTGAQGHHVHRAGRVDVELEFSESKLDGVWIVGQAVEI
                     FETEMTFP"
BASE COUNT          453 a          826 c          895 g          454 t
ORIGIN      
        1 gcttgcggcg ttgaagcctc cggtgttctg gggccgcggc acgaatgacg acgtcatccc
       61 ggagtttctc gtcgaccaca cgaccgagtg gctgccagga cacgcagagc tgagcggacg
      121 ggtgtatccc ggcctcaccc acagcgtctc ggagcaggag ctcgacgacg tgcgcgtctt
      181 tctcgacaag cagctcgagg cgatgccgac cctgggcggc tgacgccaga ccgcaccggc
      241 aggcggctac gagggcccga aacacggcct gatccgctat cgtggctgtc gaccttgttc
      301 cgagtgtgag gaagtccgtt gaagcagatc gtcgcgaccg cgctgtaacc gcgccgctcc
      361 gtcgtttctc gacgttgtcg gcgcacccgt ttccgcgctg accgtcgaat ctgccgctcc
      421 actctctcgg cctctgccgg ttcacgccgg tcgtcgggcg cttcgcggcc tcggtgtcag
      481 cgcatcactg acaccccgga ggcctcatgc ccgctcacac tctcacccct tccatcatcc
      541 tcgaccgcgt atcgttcgcg tggccggacg gttcacccgc cctcacgggc ctttccggcg
      601 ccttcggcga cggccgcacc ggactcgtcg gccgcaacgg ctcgggaaag tccacgctgc
      661 tgcggactat ctacggcgat catgccgtgg catcgggtgg cagcatcgtg cgcgctggca
      721 tcgtgccggg cgttgcgctg gaaaaattca aactgcgcac ggcctacgtc gcgccgcatc
      781 tgcaaacctg gcacatgccc aaaatgccgg tgatggaagt ggtggcatcg gggcgatacg
      841 cgagcatcgg gctcaacgat tcgatcaccg cccgtgaccg caaacatgcg gaacgcgcac
      901 tgcggcgctt cggaatgttc gcgctgcgca aacgaacgct ggcggaaatg tcgtatggcc
      961 aggcgcggcg catcctgttc gcgcgtgcgt gggcgcgaga gcctgcgctg gcgttgctcg
     1021 acgagccgtt cgccggcctg gatcgcgcga cgcgcgcgga cctgacccgg cgtctcaatg
     1081 aatggctggc ggaaggcggg agctgcgtga tcgccacgca tcatcgcgag gagtggccgg
     1141 cgcatacgac ccatgttctg gaactgaaag cgggacgcgc aatcgcgagc cgcgaagtgg
     1201 gtgaggcatg aagaagttgg ggctgctggc ggtggtggcg gtaatcgccg cgattgcctt
     1261 cgtgatgttt cggtccggcg agagcgcgcc cgaacgcatc gtcgtcaagc tcgtgcccgg
     1321 tccaccgcaa cccaaggtca ctcccgctga agccggcatc gacatggcgg caatccaggc
     1381 ggccgtggac tatgccggca agcgcaatac atcggcgctg ctcatcgggc agggcgggca
     1441 cctggtgttc gagaaatatt ggggcgatgg cacgctcgac acaccgcttt cggcgagcca
     1501 gttcggccgc gtgctggttc ccctgctggt gggctcggcc atgaacgacc ggctcatcat
     1561 cagcctggat gagcccgtgt ctaactatct gccggagtac gccgatacgc cggcgggcaa
     1621 ggcgacgctg cgcgaccacc tcgggcagcg cgtcgaaggc tccatgcccg gctcctggga
     1681 gatgctggcg ctcgtgctcg agcgcgtcac gaagcaatcg tttgacgtgc tggtgaccga
     1741 gcgtctctgg aagccgctcg aggccggaga gttctcgatg gagcgcaatg gcgacaacga
     1801 agtgcgaaag ggcgcggcga aggccgggtg ctgcgtggtt gcgagaatcg gcgactggat
     1861 gcgcatcggc gagatgctgg ccaatgacgg cgtgttcgaa ggcaaccagt tcgcgccgcc
     1921 gcgatacgtc acgctcatgc agaagccgac tcacaacctg tcgcgattgg gtctgggcat
     1981 gaacgtcggc ggtgactttg ccgccgatgt ggcgtggctg gccgacggaa ctcaacggct
     2041 ttgggtggtg ccgtcgctgc gcctggtgat cctgcgcgtg ggcgatgccc cggatgaagc
     2101 ggacggctgg gacgaggtga tgattcccga cagtatcatc cgtgggacga gcggctggca
     2161 gcccaggtca gtgggcgagg ggctggaccc gaagaagttc gcgccccact gaaaaagggg
     2221 acagatctat ttattcacgg acgccggcgt gcgcgtgctg cgacgagagg aataaataga
     2281 tctgtcccct ttttcagggg aacgtcatct cggtctcgaa gatctcgacg gcctggccca
     2341 cgatccacac gccatcgagt ttggattcgc tgaactcgag ttcgacatcg acgcgtccgg
     2401 cgcgatgcac gtggtggccc tgtgctccgg tgaactgcgc catgggacct gcatgcggca
     2461 gcaggccctg ctcggcgaga tacgcgccta acaacccgtg agcgttgccg ctgaccggat
     2521 cttcggggat gccgagcgcc gggcagaaca tgcgcgactc ggttaggcaa ctcgtgaaac
     2581 cgtgcgacag cgtgaagacg aagaagccgg cggcgccaag ctgcgcgg
//
LOCUS       DATDEU010000333         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_790697, whole genome shotgun sequence.
ACCESSION   DATDEU010000333 DATDEU010000000
VERSION     DATDEU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790697"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VIV63_07950"
     CDS             complement(<1..1074)
                     /locus_tag="VIV63_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY6124570.1"
                     /translation="MIKITDAPAELADASVVGHKFARQQQLRDAGVSVPPFFCVVRET
                     PWTAIADVVGTFPGVGASVEKLTAWAEAARSAAEHVRLMPETETEIRDRYAALGSPDV
                     AVRACVVGRHAQPGEDDAADPFAGLTDSYLYVGAEALIGAVSACTASLFSVRSVLYRA
                     RRGIDARGLSICVGIQRMMDGERSFVAFTHDPATGARETVVAAVYGIGEGAVAERADI
                     DHFFVRPGGIERLISHKKRMLRRAPGGGVAEYRVPDGLADLPVFSDAQISQIAALAEA
                     TERLLSGPQDIEGVVTADLAIHLVQARPATSPQTPGTTIEWTNHNLTESFPGVTTAMT
                     YSHARVFYRMSFRSFYRIVGVPET"
     gene            complement(1074..1697)
                     /locus_tag="VIV63_07955"
     CDS             complement(1074..1697)
                     /locus_tag="VIV63_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY6124571.1"
                     /translation="MTDRVAMYILRTLLALTAIGLTIAVATHLVGGRLAAAAFVAAYL
                     VWLLSEARITVGTPTQSAAEARTLVPYAVARVTTALTAAYSAPAVSDKFGIALAAVFV
                     AGVTLRAWAIHELGVQYSHRVLRISEGGLISSGPYGVLRHPAYAGMLLANIGFAVYFS
                     SPASIAALTVLVAAVLWRIRIEEDILTESPQYRAFANTRCRIVPGVW"
     gene            complement(1722..>2318)
                     /locus_tag="VIV63_07960"
     CDS             complement(1722..>2318)
                     /locus_tag="VIV63_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124572.1"
                     /translation="CELDAPVEVSATRETVGNLFEFIQSGRSIVRGLLGRRETALPAE
                     LAPAPAFGNGSIPSELVHRADPGNIAVGPLTVEAGRVWVRVHEQAAIGRIPPRASAVA
                     SAIEAARQFVIAILHLWGQQPMGSKMIFVGLTAEVPTAVPQGHMTLALSWQPTPPEQT
                     RKLHIDLHAVGDRAIKVGSIVIATRCVDEVVYAPLRAG"
BASE COUNT          448 a          717 c          732 g          421 t
ORIGIN      
        1 ggtctcaggg actccgacga tgcgatagaa gctgcggaaa ctcatgcggt agaagactcg
       61 tgcgtgcgag tacgtcatcg cggtggtcac acccggaaag ctctcagtca gattgtgatt
      121 ggtccactcg atcgtggttc ccggcgtttg gggcgacgtg gcgggccgtg cctgcaccag
      181 gtgaattgct aagtcggccg tgacgacgcc ctctatgtcc tggggtccgg acaacaaccg
      241 ctcggtggcc tccgccagcg ctgcgatctg gctgatttgt gcatcggaga acaccggtag
      301 gtcggccagt ccatccggca cccgatattc ggcaacgcca ccgcccggcg ctcggcggag
      361 cattcgtttc ttgtgagaga tgagcctctc gataccgccc gggcgtacga agaagtggtc
      421 gatgtcggca cgttcggcta ccgcaccttc gccgatgccg tagaccgcag cgaccacagt
      481 ttctctggct ccggtagcag gatcatgggt gaacgcgaca aacgaccgct cgccatccat
      541 cattcgctga attccgacgc agatgctgag gcctcgggcg tcgatccctc gccgcgcgcg
      601 gtagagcacc gatctgacgc tgaaaagtga tgcggtgcag gccgaaacgg cgccgatgag
      661 cgcttcggca ccaacgtaca ggtaactatc tgtgaggccc gcgaacgggt cggcggcgtc
      721 atcttcgccc ggctgtgcgt ggcggccgac cacgcatgct cggactgcga cgtcgggtga
      781 gccgagggcg gcgtatctgt cccggatctc ggtttctgtc tccggcataa ggcgcacgtg
      841 ttcggcagcg ctgcgagccg cctcggccca ggccgtcaat ttctcgacac tggcgccgac
      901 accaggaaac gtgccaacca catcggcgat ggctgtccac ggagtttctc tgacgacgca
      961 gaagaacggc gggaccgaaa ctccggcatc gcgtaactgt tgctggcgtg cgaacttgtg
     1021 cccaaccacg ctagcgtcag ccagttctgc aggtgcgtca gtgatcttga tcatcaccac
     1081 acccccggca caatccggca tcgtgtgttg gcgaaggcgc gatattgggg cgattcagtg
     1141 aggatgtctt cttcgatcct gatgcgccag agaaccgcgg cgacaaggac agtcagtgcc
     1201 gcgatactcg caggactcga aaagtaaacg gcaaaaccga tgttggcgag caacatcccg
     1261 gcataagcag gatggcgcag caccccatac ggcccggatg atatgaggcc accctcggag
     1321 atccgtaaca ctcggtggga atattggacg ccgagttcgt gaatcgccca tgcgcgtaac
     1381 gtcacaccag caacgaaaac cgccgcgagc gcgataccga acttgtcgga aaccgcgggc
     1441 gcggagtacg cagccgtaag tgccgtcgtg actcgcgcta ccgcgtatgg aacgagggtg
     1501 cgagcctccg cggccgactg cgttggtgtc ccgacagtga ttcgtgcttc ggaaagcagc
     1561 cacaccagat atgcggccac gaaagccgct gcagcgaggc gcccgccgac aaggtgtgtc
     1621 gcgaccgcaa tggtaaggcc gatagcggtc agtgccaaca gagtccgaag aatgtacatg
     1681 gcgactcgat cggtcattac cagcctccat cgagtcagtg tctagcccgc gcgcaagggc
     1741 gcatacacga cttcgtcgac gcaccgggtc gcgatgacga tcgagccgac tttgatcgct
     1801 cgatcgccta ctgcgtgcag gtcgatgtgg agtttgcgcg tctgttctgg cggagttggc
     1861 tgccacgaca acgccagcgt catgtgaccc tgcggcaccg cagtgggcac ctctgcggtg
     1921 agcccgacga atatcatctt tgaccccatt ggctgctgac cccataagtg aagaatcgcg
     1981 atgacgaact ggcgggcggc ctcgatagcg gaggcgaccg cgctggcccg cggcggtatc
     2041 ctgccgatgg cagcctgctc atgtacgcgg acccaaacgc gtccggcctc tactgtgagc
     2101 gggccgactg cgatatttcc ggggtcggcc cggtgcacca gttcactcgg gatcgagccg
     2161 ttcccaaatg ctggcgccgg ggcaagctct gcaggcagcg cggtctcgcg tcggcccagc
     2221 agtcccctta ctatgctgcg gcctgattgg atgaattcga ataggtttcc aacggtttcg
     2281 cgggtcgcac tcacctcaac cggggcgtcg agctcgca
//
LOCUS       DATDEU010000334         2562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_60862, whole genome shotgun sequence.
ACCESSION   DATDEU010000334 DATDEU010000000
VERSION     DATDEU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60862"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /gene="gltX"
                     /locus_tag="VIV63_07965"
     CDS             <1..1403
                     /gene="gltX"
                     /locus_tag="VIV63_07965"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY6124573.1"
                     /translation="QGPITTRFAPSPTGELHLGNARTALFSWLFARHHGGRFVLRIED
                     TDTERSKEAYTTALMEDLQWMGLQWDAGPGRAREGEEYRQSRRGQVYARHSATLDASG
                     LTYPCYCTPLELDLSRKAQLAAGRPPRYAGTCRELSAEARVRKSAQGISPTTRFRVPT
                     GRRVEFDDMVHGAQSFATDDIGDFILRRADGSAAFFFSNAIDDAEMGITHVLRGEDHL
                     TNTPRQLLVLEALGLRAPTYGHLSLLVGADGSPLSKRHGATSVREFRDRGYLPAALAN
                     HLFRLGHSSGENGVLSLDAMAKHFTVKHLGRAPARFEESQLNVWQREVAHHMPLEESE
                     RWLSAETPGDIDVERRRAFIAAIRPNVLLPRDVAQWREVVFGPSPLLEGDALARVREA
                     GPQFFRAAAEAAASDPSLDAIVSAVKAATGAKGQALWKPLRLALTGAPEGPELAPLLK
                     AMAAPSIHERLSRFAA"
     gene            1400..>2562
                     /gene="cysS"
                     /locus_tag="VIV63_07970"
     CDS             1400..>2562
                     /gene="cysS"
                     /locus_tag="VIV63_07970"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY6124574.1"
                     /translation="MIRIHNSLTGEKQELVPVEPGHVRMYSCGVTVYDFFHVGNARML
                     VVFDMVSRYLRHRGLRVTYVRNITDVDDKIINRAREAGIDWRVHAKKFTDAMHEDLDQ
                     LSCLRPEKEPKASEYIDEMLKMISQLIDNGFAYQASNGDVMYDVHKFGPYGRLSGKKL
                     EDLRAGSRVEVDSAKRDPLDFVLWKSAKPGEPSWPSPFGDGRPGWHIECSAMSTKELG
                     EHFDIHGGGLDLKFPHHENEIAQTCGATGGKFAEIWMHNGFLNIDNEKMSKSLGNFFT
                     TREVLAKIRHPEVLRFFLLSSHYRGPMNYSPDQLDQAEAGLNRLYTALRDVPEVGGEL
                     YSPTAATRAFDVAMDDDFNTPEAIAALQGLARELNSVRKPDADSHKAKLLAGEL"
BASE COUNT          455 a          874 c          824 g          409 t
ORIGIN      
        1 cacagggacc catcaccact cgcttcgcgc ccagccccac gggcgagctc catctcggca
       61 acgcgcgcac cgcgctgttc agctggctgt tcgcgcgcca tcacggcgga cgcttcgttc
      121 tgcgcatcga ggacacggat accgaacgca gcaaggaggc ttacaccacc gcgctcatgg
      181 aagacctcca gtggatggga ttgcagtggg acgcgggtcc cggccgcgcc cgagagggcg
      241 aggaataccg gcagtcgcgc cgcggccagg tctacgcgcg ccattcggcc acgctcgacg
      301 cgagcgggct cacgtacccc tgctattgca ccccgctcga gctcgacctt tcgcgcaagg
      361 cgcaactcgc cgcgggccgg cctccgcgtt atgccggaac ctgccgcgag ctctccgccg
      421 aggcgcgggt gcgcaagagc gcgcagggca tctcgccgac cacgcgtttc cgcgtgccga
      481 ccggccggcg cgtggagttc gacgacatgg tgcacggagc ccagagcttc gccaccgacg
      541 acatcggcga tttcatcctg cggcgcgcgg atggcagcgc ggcctttttc ttcagcaatg
      601 ccatcgacga tgccgagatg ggcatcactc acgtcttgcg cggcgaagac cacctgacca
      661 ataccccgcg ccagctgctg gtgctggagg cgctgggcct gcgcgcgccc acctatgggc
      721 acctgtcgct gctggtgggc gcggatggct cgccactgtc caagcgccat ggcgcgacca
      781 gcgtgcgcga attccgcgat cgcggctatc tacccgcggc gctggccaat cacctgttcc
      841 ggctggggca ctcgagcggt gagaacggcg tgttgtcact ggacgccatg gcgaaacatt
      901 tcacggtcaa gcatctcggg cgcgcgccgg cgcgtttcga ggaatcccag ctcaatgtct
      961 ggcagcgcga agtcgcgcac cacatgccgc tggaggaaag cgagcgctgg ctgtcggccg
     1021 agacccccgg ggacatcgac gtcgagcgcc gccgggcctt catcgccgcc atccggccca
     1081 atgtgctgct gcccagggac gtggcgcagt ggcgcgaggt ggtgttcggg ccttcacccc
     1141 tgctcgaggg tgatgcgctg gcacgggtgc gcgaagccgg cccccagttc ttccgtgccg
     1201 ccgccgaagc cgcggccagc gatccctcgc tcgacgccat cgtcagcgcg gtcaaggccg
     1261 ccaccggcgc caagggccag gccctgtgga aaccgctgcg gctggcgctc acgggcgcac
     1321 ccgaggggcc cgagctcgcg ccgctgctca aggccatggc cgcgcccagc attcatgaac
     1381 gactgagccg cttcgccgca tgatccgcat tcacaattcc ctgacgggcg aaaagcagga
     1441 actcgtcccc gtcgagcccg gccacgtgcg catgtacagc tgcggcgtca ccgtctacga
     1501 cttcttccac gtgggcaatg cccgcatgct ggtcgtgttc gacatggtca gccgctacct
     1561 gcggcatcgt ggcctgcgcg tcacctacgt gcgcaacatc accgacgtgg acgacaagat
     1621 catcaaccgc gcccgtgaag ccggcatcga ctggcgcgtc cacgcgaaaa aattcacgga
     1681 tgccatgcac gaggatctcg accagctcag ctgcctgcgt ccggagaagg agcccaaggc
     1741 gtcggagtac atcgacgaga tgttgaagat gatttcccag ctcatcgaca acggttttgc
     1801 gtaccaggcg tcgaacggcg acgtgatgta cgacgtacac aagttcggtc cctatggccg
     1861 gctgtccggc aagaagctcg aagacctgcg cgccggctcg cgcgtggaag tggattcggc
     1921 caagcgcgat ccgctggact tcgtgttgtg gaagtccgcc aagcccggcg aacccagttg
     1981 gccttcaccg ttcggcgacg gccgtcccgg ctggcacatc gagtgttcgg ccatgagcac
     2041 gaaggagctg ggcgaacatt tcgacattca tggcggcggg ctggatctca aattcccgca
     2101 ccacgaaaac gagatcgcgc agacctgcgg cgccaccggc ggaaaattcg ccgagatctg
     2161 gatgcacaac ggttttctca acatcgacaa cgagaagatg tcgaagtcgc tgggcaactt
     2221 cttcaccacg cgtgaagtgc tggcgaagat ccggcatccc gaagtgctgc ggttcttcct
     2281 gctgtccagc cactaccgtg gcccgatgaa ctactcgccg gatcaactcg accaggcgga
     2341 ggcggggctc aatcgcctgt acacggcgct gcgtgacgtg ccggaagtgg gtggtgaact
     2401 gtattcgccc acggcggcca ccagggcctt cgacgtggcc atggacgatg acttcaatac
     2461 gcccgaggcg atcgccgcgc tgcagggcct ggcgcgtgag ctcaacagcg tgcgcaagcc
     2521 ggacgcggat tcgcacaagg ccaaactcct ggcgggcgag ct
//
LOCUS       DATDEU010000335         3359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1228095, whole genome shotgun sequence.
ACCESSION   DATDEU010000335 DATDEU010000000
VERSION     DATDEU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228095"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VIV63_07975"
     CDS             <1..355
                     /locus_tag="VIV63_07975"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HEY6124575.1"
                     /translation="ALAITSAVKAVKVKQVGFSGLMLPVMEDKRLAQRWSENTYDIDS
                     VLAYSAVCGTGLDTVPLPGDVSEERLVKIFGDVAALAWKWNKPLSARLQPVSGKKTGE
                     MTEFESQYLFNTTLH"
     gene            457..906
                     /locus_tag="VIV63_07980"
     CDS             457..906
                     /locus_tag="VIV63_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124576.1"
                     /translation="MNTLRGAVSSARAITSVSGGGDNSPVQTTHILLFKVDGRQVKLS
                     SASPAMISDNDIVVLAGIEKDGAFVALAYRNETTGVIGNAGKLGPAIGVLVGLVGAAF
                     AFIVFGSSSFGVVPKLIGLVFLAVATFSAIRFMKIKSAADLLAATRS"
     gene            1007..1651
                     /locus_tag="VIV63_07985"
     CDS             1007..1651
                     /locus_tag="VIV63_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6124577.1"
                     /translation="MKKHRKARGWTQEQLAIVANVTVRTIQRLESGESPAPETLVAVA
                     QAFGMKAEHLNPTSDSVSDAASRRYVHLLPRLATVSDLTAVISRAHQCQYEHDDTADP
                     RSITTMKDILEAVGEDLARLQDARPEERPVIEAELSRELEALQSLGYYLFGIMRVVPK
                     FFDEHVMLMSVATIYITHSRSPKIVKANNMMVIPAILPEEVTFIGDPAFVLPVY"
     gene            complement(1663..2454)
                     /locus_tag="VIV63_07990"
     CDS             complement(1663..2454)
                     /locus_tag="VIV63_07990"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY6124578.1"
                     /translation="MKTKKEIVDNWLPRYTGTPIESFGEYFLLTNFGNYLDIFAERTG
                     AEIMGLGRPMPNATSDGISVINFGMGSANAATVMDLLSARMPKAVLLLGKCGGLKKKV
                     GIGDLVLPIAAIRGDGTSNDYLLPEVPALPAFSLQRAVSTVIRDLGLDYWTGTIYTTN
                     RRVWEHDGEFKDYLRRTRAMAIDMETATIFAAGFANRIPTGALMLVSDQPMVPEGVKT
                     EASDRKVTDNFVQRHIEIGVEALKLIRRHGKSVRHLRFEDGSGGD"
     gene            complement(2552..>3359)
                     /locus_tag="VIV63_07995"
     CDS             complement(2552..>3359)
                     /locus_tag="VIV63_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEY6124579.1"
                     /translation="EYLPRIYPVRRLLPDLKFLATERISFDTPSFPIFEPDPPAGASC
                     FNSEAEEDGAQDCLRFDQIIANVGKGPVEIAYQVPAGETPSEDEPFPVSQRVYNSDGN
                     FVDQPGGSVNFHGVHGHFHYSSFANALLWQSNKKGTKLGSAPVAAAHKVSFCMADIRI
                     DAWGKKGDGPRKYYAPDCLFPFASDGVTDEYRQGINNGWSDVYDWYIPDQYVEVSGIV
                     DGYYLLEFCADPFNEIEEEREDNNCATNHIRLSGMGTADKSVVVVLPQKR"
BASE COUNT          649 a         1004 c         1013 g          693 t
ORIGIN      
        1 ggcgctggcc atcacctcgg ccgtcaaagc cgtgaaggtg aagcaggtcg gtttttcagg
       61 attgatgctg ccggtgatgg aagacaagcg tctcgcgcaa cgctggtctg agaacactta
      121 cgacatcgat tcggtgctcg cgtattccgc ggtgtgcggc acggggctcg acacggttcc
      181 gctgccgggc gacgtcagcg aggaacgtct ggtgaagatc ttcggcgacg tcgcggcgct
      241 ggcgtggaaa tggaacaagc cactctcggc gcgcctgcaa ccggtcagcg gcaagaagac
      301 gggcgagatg acggagttcg aaagtcagta cttgttcaat acgacgctgc actgagaagc
      361 gccgaacggc gtttccatta cagcgctcgc tatctattca cgcctgccat acgattccga
      421 ccctggaggc gacatgcgta cttgggaagg aattccgtga acacactcag gggcgcagtc
      481 tcttcagcgc gggctatcac ttcggtttcc ggtggcggcg ataattcacc ggttcaaact
      541 acccacatcc tgcttttcaa agtggacggg cgccaggtaa agctctcatc ggcgagcccc
      601 gccatgatca gtgacaacga catcgtcgtc ttggccggca tcgagaagga tggcgccttc
      661 gtcgccttgg cctatcgcaa tgagacaacg ggagtgatcg gcaacgccgg aaaactgggg
      721 cccgcaatcg gtgtgctggt cggcctcgtc ggggccgcct tcgcgttcat agtgttcggg
      781 agttcctcgt ttggcgtcgt ccccaaactg attgggttgg tattccttgc ggtagcaacc
      841 ttcagcgcca tccgcttcat gaagatcaaa tccgcggccg atctgctcgc ggcgacccgc
      901 tcgtaggagt cgaggcccgg tccagtgtcg tgcccgtgtc gtgttcacgc gccccgctaa
      961 ctcgtaatct gcccgcacca tgtcagacac cgagagaggg caagtcatga agaaacatcg
     1021 caaggctcgc ggttggactc aggagcagct cgccatcgtc gccaacgtga cggtgcgtac
     1081 gatccagcgg ctcgagagcg gcgaatcacc ggcgccagag accttggtgg cggtggcgca
     1141 agccttcggc atgaaagcgg agcatctcaa tccgacatcg gattccgttt cggatgcggc
     1201 ctcgcgaagg tacgttcatt tactcccacg actggcgacc gtcagtgatc tgaccgccgt
     1261 catctcgaga gctcaccagt gtcaatacga gcacgatgac accgcggatc cgcggtcgat
     1321 caccacgatg aaggatattc tcgaggcggt cggcgaggat ctggcgcggt tgcaggacgc
     1381 caggccagaa gaaagacccg tcatcgaggc agagctgtcg agagagctcg aggcattgca
     1441 gagtcttggg tactacctgt tcgggatcat gcgggtagtc cccaagttct tcgacgagca
     1501 cgtcatgctg atgtccgtgg caacgatcta catcacccat tctcgatcgc cgaagatcgt
     1561 gaaggccaac aacatgatgg tgattcccgc gatcttgccc gaagaggtaa cgttcatcgg
     1621 cgacccggca ttcgtgctgc cagtctactg actggctact ggctagtcgc cgccagaccc
     1681 atcttcaaac cgcagatgcc tgacgctctt cccatgccgc cggatcagct ttagcgcttc
     1741 gactcctatc tcaatgtgcc tctgcacgaa gttgtcggtg accttccgat cagaagcctc
     1801 ggtcttcact ccctccggca ccatgggttg atcactgacg agcatcagcg ccccggtcgg
     1861 aatgcgattg gcgaaacccg ccgcaaaaat tgtcgcggtt tccatatcga tggccatagc
     1921 gcgagtgcgg cgaagatagt ccttgaactc accatcgtgc tcccagacgc gccgattggt
     1981 cgtgtagatg gtgcccgtcc agtagtcgag ccccagatcg cggatcaccg tcgacacggc
     2041 tctctgcaga ctgaacgcgg gcagcgccgg cacctcggga agcaggtagt cgttgctggt
     2101 tccgtcgcct cggatggccg caatcggcaa taccaggtcg ccgataccca ccttcttctt
     2161 taacccgccg cacttgccga gcagtagaac ggctttcggc atccgcgcag acaacaagtc
     2221 catgacggtg gcggcgttgg cgctgcccat gccgaagttt attaccgaga tcccgtcaga
     2281 cgtcgcgttc ggcatggggc ggccgaggcc catgatctcg gcgcccgtgc gctccgcgaa
     2341 gatatcgagg tagttgccga agttcgtcag caggaagtat tcgccgaaac tctcgatggg
     2401 cgtgcccgtg tatcgaggca gccagttgtc cacgatctct ttctttgttt tcacgatcaa
     2461 ggccttccgc aagtcgatgc agggatgcca ttgaacctgt cggcgacgtg atcttcaaca
     2521 atacctgttc aacccgatgg tacggctgcc ctcaccgctt ctgcggaagc accaccacca
     2581 cgctcttgtc cgccgtcccc atgccgctca agcggatgtg gttggtcgca cagttgttgt
     2641 cttcgcgttc ttcctcgatt tcgttgaagg ggtcggcgca gaattccagc aggtagtagc
     2701 cgtcgacgat gccgctgact tccacgtact ggtcagggat gtaccagtcg tacacatccg
     2761 accaaccgtt gttgatgccc tggcgatact catcggtgac accgtcgctg gcgaatggaa
     2821 acaggcaatc cggtgcgtag tacttgcgcg ggccgtcgcc cttcttgccc caggcatcga
     2881 tgcggatgtc ggccatgcag aagctgacct tgtgcgcggc ggccaccggc gcgctgccca
     2941 gcttcgtgcc cttcttgttg gactgccata acagcgcgtt ggcgaaactc gagtagtgga
     3001 agtgcccgtg aactccgtga aagttcacgc tgccgccagg ctggtcgacg aaattgccgt
     3061 cgctgttgta cacgcgttgc gaaaccggaa acggctcgtc ttcactgggc gtctcgccgg
     3121 cagggacctg gtaggcaatt tccacgggac ccttgccgac gttggcgatg atctggtcga
     3181 agcgcaggca atcctgcgcg ccgtcttctt cagcttcgct gttgaagcag ctggcgccgg
     3241 cgggcggatc cggttcgaag atcgggaagg agggtgtgtc gaagctgatg cgttcggtgg
     3301 cgaggaactt gaggtccggc agcaggcgac ggactggata gatgcgcggc aggtactca
//
LOCUS       DATDEU010000336         4643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1520295, whole genome shotgun sequence.
ACCESSION   DATDEU010000336 DATDEU010000000
VERSION     DATDEU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520295"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VIV63_08000"
     CDS             complement(<1..1091)
                     /locus_tag="VIV63_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6124580.1"
                     /translation="MTHRTGTGPITRKVLIVDSGLAGTGAAARRVRALADELGTRNIS
                     VTESTSYEDGLATVVSDSSIHCLLLNWSQGGTDQHALGEATELLRTVRKRNAKIPIFL
                     MASRALSGTLNVEVMTLADEFIWILDDTATFISGRVQGAIERYAQTLLPPYFAALMRY
                     DREKEYSWAAPGHQGGTAFLKSPVGRVFFDFYGENLFRTDMGIERGALGSLLGHSGPV
                     GESERYAARVFGAARSYTVLNGTSASNRAIMSACVGDDEIALCDRNCHKSIEQGLAIT
                     GGIPVFLAPTRNRYGIIGLIPPERLEPDHIAKIIAANPLAKAAGGKRPVYSVVTNSTY
                     DGMCYNATDAQARLAKSVDRIHFDEAWYG"
     gene            complement(1198..2535)
                     /gene="guaD"
                     /locus_tag="VIV63_08005"
     CDS             complement(1198..2535)
                     /gene="guaD"
                     /locus_tag="VIV63_08005"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEY6124581.1"
                     /translation="MSTSTAIRGPVLTYTGDAFLDGVESTMRYERDAIVAMAGGKITH
                     FGPASEVRAQLPAGTVIREYGSDSLIMAGFIDCHVHYPQTQIVGAFGKQLIDWLNQYT
                     FIAEQQFAVEEHARAVARVFLRECLRAGTTSAAVYCTVHPQSVDAFFEESGKLGMRMI
                     AGKVLMDRHAPEALTDTPQKGYDESRALIAKWHGKHRNLYAVTPRFAASSSPAQMEMA
                     GALWHEHPGTYLQSHVSENRDEVSWVKQLYPERAGYLDVYDHYGQLGPRSIYGHGIWL
                     TEQELLRCHESGTAIAHCPTSNLFLGSGLFNMASAMNPGRPVRVGLATDIGAGTSFSM
                     LQTLNEAYKVAQLIGNSLSGAQAFYLATRGTAKALYLDDKIGSIAPGMEADVIVLDLN
                     STPLIDFRMQHCRDIDEALFVLMTMGDDRAVKATYISGQLQYQRPDYPTQSAI"
     gene            complement(2547..3104)
                     /locus_tag="VIV63_08010"
     CDS             complement(2547..3104)
                     /locus_tag="VIV63_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY6124582.1"
                     /translation="MSGSLPAAEPPKPLPVLVATVSKVNDGDSIEVNLDSGPGRVRFS
                     AIDTPEYDQPYGPQSSSALKAMLPVGSVVELEVVTQDQFHRFVATVWLVSDGKRTNIN
                     EAMLRDGHAWAYRRYMKDPRFCDIEAEARDNKRGLWAQPVSEWVYPPEWRLLKNGEIR
                     TLPAPYPETREKCVAVLGLAGSATY"
     gene            3488..4643
                     /locus_tag="VIV63_08015"
                     /pseudo
     CDS             3488..4643
                     /locus_tag="VIV63_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
BASE COUNT          840 a         1438 c         1456 g          909 t
ORIGIN      
        1 tagccgtacc acgcttcgtc gaaatgaatg cggtccacgc tcttggcgag ccgcgcctgc
       61 gcgtcggtcg cgttgtagca catgccgtca tacgtgctgt tcgtgaccac ggagtacacg
      121 ggccgcttgc cgcctgcggc cttggccagc ggattggcgg caatgatctt cgcgatgtgg
      181 tcgggctcga gtcgttccgg cggaatcagt ccgatgatcc cgtagcgatt acgcgtgggc
      241 gccaggaaca ccgggatgcc accggtgatc gccaggccct gctcgatcga cttgtggcag
      301 ttgcgatcgc acagcgcgat ctcatcgtcg cccacgcacg ccgacatgat ggcgcggttg
      361 gaagccgagg tgccgttcag caccgtatac gatcgggccg cgccgaagac gcgtgcggcg
      421 taacgctcgc tctcgcccac cggcccgctg tgccctaaca aagagcccag cgcaccgcgc
      481 tcgatgccca tgtccgtgcg gaacaggttc tcgccgtaga aatcgaagaa cacgcggccg
      541 acgggcgact tgaggaacgc cgtgccgccc tggtgacccg gcgccgccca cgaatattcc
      601 ttctcgcggt cataccgcat cagcgccgcg aagtatggcg gcagcagcgt ttgtgcataa
      661 cgctcgatcg cgccctgcac gcgtccgctg atgaaggtgg cggtgtcgtc gagaatccag
      721 atgaactcgt cggcgagcgt catcacctcg acgttcagcg ttccggagag tgcgcggctg
      781 gccatgagaa agatgggtat cttggcgttg cgcttgcgca cggtacgcag cagttcggtc
      841 gcttcgccaa gggcgtgctg gtcggtgcca ccctgggacc aattgagcag caggcaatga
      901 atgctggaat cggacacgac ggtcgcgagt ccatcttcat aagacgtgga ctccgtgacg
      961 gaaatgttcc gggttccgag ctcatcggcg agcgcgcgaa ccctgcgcgc ggcggcgccc
     1021 gtgccagcga gaccactgtc gacgatcagg accttgcggg tgatcggtcc ggttccggtt
     1081 cggtgcgtca tgtcgattcc ttcgagcgac ggctctaaaa cgacgatgac tgtaggaatc
     1141 caccgtaggt cggtatatgg gacctagggg ctagcgctgg cggcgcgagt gcgggattta
     1201 gatagcgctt tgtgtcgggt agtcaggccg ctgatattgg agctgtcccg aaatgtacgt
     1261 cgccttcacg gcgcggtcgt cgcccatcgt catcaggacg aaaagagctt catcgatgtc
     1321 gcggcaatgt tgcattcgga aatcgatgag cggagtcgag ttgagatcga gcacgatgac
     1381 gtcggcttcc atgccaggtg caatgcttcc gatcttgtcg tcgagataca gcgccttcgc
     1441 ggtaccacgc gtggcgaggt agaacgcctg agcgcccgac agcgagttgc cgatcagctg
     1501 cgcgaccttg tacgcctcgt tgagggtttg cagcatcgag aacgatgtgc cggcgccgat
     1561 gtccgtcgcc agtcccacgc gcaccggccg cccgggattc atggcactgg ccatattgaa
     1621 gagcccgctg ccgagaaaca ggttggatgt cgggcaatgc gctatggcgg tgccggactc
     1681 atgacagcgc aggagttcct gctcggtgag ccagatgccg tggccgtaga tagaccgtgg
     1741 tccgagctgg ccgtagtgat cgtagacatc gaggtagccg gcacgctccg gatacaactg
     1801 cttcacccat gagacttcat cgcgattctc ggacacgtgt gactgaaggt acgtacccgg
     1861 atgttcgtgc cacaacgctc cggccatttc catctgcgcg ggagagctgc tcgccgcgaa
     1921 tcgcggagtc acggcgtaca gattccggtg cttgccatgc catttcgcaa tgagcgcccg
     1981 ggattcgtcg taacccttct gtggtgtatc ggtgagcgcc tcgggcgcat gacgatccat
     2041 gagcaccttg cccgcgatca tccgcattcc caacttgccg gactcctcga agaacgcatc
     2101 gaccgactgc ggatgcaccg tgcagtagac cgcggcggag gtcgtaccgg cgcgcaggca
     2161 ttcccggagg aacacgcgtg ccaccgcgcg cgcatgttcc tcgacagcga actgctgctc
     2221 ggcgatgaat gtgtactggt tcagccagtc gatgagttgt ttgccgaagg cgccgacgat
     2281 ctgcgtctgg ggatagtgca cgtggcaatc gatgaacccg gccatgatca gcgaatcgct
     2341 gccgtactcg cggatcaccg tgccagccgg cagttgcgcg cgcacttcac tcgccggacc
     2401 gaaatgggtg atcttgcccc cggccattgc cacgatcgcg tcgcgttcat aacgcatcgt
     2461 gctctcgacg ccgtcgagga acgcatctcc cgtgtacgtc aggaccgggc cgcgaatcgc
     2521 agtcgaagtg ctcatcgaat tcaaggctag taggtggccg acccggcgag ccccagaacc
     2581 gccacgcact tctcacgtgt ttccggatac ggcgcgggca acgttcggat ctcgccgttc
     2641 ttgagtaacc gccactcggg tggatagacc cattcgctga cgggttgagc ccacagtccg
     2701 cgtttgttgt cacgagcctc ggcttcgatg tcgcagaacc tcggatcttt catgtaacgc
     2761 cggtacgccc aggcatgacc gtcgcgcaac atcgcttcat tgatgttggt gcgtttgccg
     2821 tccgacacca gccagacggt ggcgacgaac cggtgaaact ggtcctgggt gaccacttcg
     2881 agctccacga ccgaccccac gggcaacatt gctttcagcg cagaggacga ctgcggaccg
     2941 tacggctggt cgtactccgg cgtgtcgatg gccgagaaac gcactcgccc ggggccggaa
     3001 tcgaggttta cttcgatgga atcgccatcg ttgaccttgc tgaccgtggc gacgagtacg
     3061 ggcagcggct tgggaggctc ggccgcaggt aaacttccgg acaaagtcaa gagccagaga
     3121 accccggttt ttttatgcat ggcccacggt ttagcacacc gcgatgaaaa aaaaaccttc
     3181 gccgatagag ggggatcatc ggcgaaggtt cgcaatgaac cccgcctggg ggttccatgt
     3241 ccgagtgcgt cgagtcttcc ccactcctaa cagggttaca tcagtatttg tatctaactg
     3301 cccgcatcgc gaagagcttg tgccgtgcag cagcctggtt aggccgcgca ggttcatgca
     3361 tacgtattcc aacgtcgaac accgtgtgcg cgggtcatac tgagtcgtgc aattgaagtc
     3421 gcagcgcccc gccgaatcgg cgccagtacg ttccgtggtc atcgtgggcg gtggcaccgc
     3481 cggttggatg accgccgcct cgctcgccca tcggctgcag cggctgggtg tctcgatcac
     3541 cgtcatcgaa tcgtcatcga tcggcaccat cggtgtcggc gaagccacgg tgccggcgat
     3601 ccgtcgctac tttcaatcgc tcggtctcga cacgttcgag gtcatgaagg ccacgaacgg
     3661 ctcgatcaag ctgggaatcg aattcgacgg ctggcgacat gcgggccatt cgttcatgca
     3721 tccattcggc cgcttcggtc tcgaagccgg tcccgtggcg ttccacgatc tctggaaccg
     3781 cttgagccgc gagtcgaatg gcgatccgaa tttcgcgggg acgctcgacc actagtccat
     3841 gggcacgcaa ctcgcgcgcg cgggccgcat gaccctgcct cctgaaaacc cgcgcgtgga
     3901 tttcgagaac ttcgattggg ccgtgcattt cgacgccgga cggtttgcga cgttcctgcg
     3961 cgggttcgca gaagcgcgag gtgtgcggcg catcgacgcg cgcgtggccg aagtgctgcg
     4021 cgacggtgaa acggaaactc ttcgcggcgt ccggctcgat tccggggaca tcctcgaggc
     4081 ggcgctgttc atcgactgca gcggatttca ccgtctgctc atcgaccgca cgctgaacgc
     4141 gggtttcgtc gactggggtc actggctcaa atgcgaccgc gccgtcgcgc tgccctgctc
     4201 ggccgccgaa aactctttca tgccatacac gcggtcgcga gcgatggacg caggctggac
     4261 ctggcgaatc ccgttgcaga atcgcgtggg caacggctac gtgtattcct cgcagcacat
     4321 cggcgacgag caggcgctcg cggccttacg cgcgtcgctc gaaggcccgg tgctcggcga
     4381 gcccaacttc gtgaagtttc gcaccggtca tgtgcgccgc ttctggatcg gcaattgcgt
     4441 cgccatcggc ctctccggtg gcttcctcga gccgctggaa tccaccagca tctcgctgat
     4501 ccagatgggt atcgacaaac tactgcactt ctggccggac gaggggatgc ccgccgagct
     4561 cgcggcccag ctctcgggcg aatacaaccg gctgtcggtc gtcgaattcg agcggattcg
     4621 tgatttcatc atccttcact aca
//
LOCUS       DATDEU010000337         3454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_645195, whole genome shotgun sequence.
ACCESSION   DATDEU010000337 DATDEU010000000
VERSION     DATDEU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645195"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..789
                     /locus_tag="VIV63_08020"
     CDS             70..789
                     /locus_tag="VIV63_08020"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6124583.1"
                     /translation="MNYNAALYGFGALLLGAVGIYFHDFAMQWQPVPAGIAGRTALAY
                     ASGALLVVCGAAILLRRYERIGVLLLASFYWLWMLVLNLPLAIKSYTHIGAWNSPAEI
                     AFLAAGGTALVATSAGQLRNTLTLVARIVAGVSALVFGLAHFNYAEFTASMVPAWIPY
                     KLFWAYATGAGHFAAGLALVSGIRARLAATLLAGMMGSFVVLLHIPRVIASPDQHVEW
                     IMLAIASSLSGAALLVRKYAT"
     gene            complement(770..1429)
                     /locus_tag="VIV63_08025"
     CDS             complement(770..1429)
                     /locus_tag="VIV63_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124584.1"
                     /translation="MKNTSTLPLGLKIFLAAFSLTLIAYNTLFQGVHSLLYFCNVALF
                     LGTIGICLERALPVAMAAVGVVMIQLLWSVDLALTAAEVSPVRMTAFMLNDNLPVFKK
                     VLALFHAWLPFVLIFAMSRLGYDPRALVSWTVVTWALLLVSYLFLPRPPAPPDDPTLS
                     VNINFVFGLNMAGPQQRMPELAWLGAMMLALPLLVFLPSHFVFKRLFGGEPAAVTLRT
                     S"
     gene            1576..1836
                     /locus_tag="VIV63_08030"
     CDS             1576..1836
                     /locus_tag="VIV63_08030"
                     /inference="COORDINATES: protein motif:HMM:NF018500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HEY6124585.1"
                     /translation="MSDDTDSPAGPPIQVSYRDLSSGALQGVIESYVLREGTDYGERD
                     VPFESKVAQVRRQLETGEAAIYFDPETESIDIKPVTGARSAR"
     gene            1939..3048
                     /locus_tag="VIV63_08035"
     CDS             1939..3048
                     /locus_tag="VIV63_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY6124586.1"
                     /translation="MKHVAIFIGAALCAAASQAMAAGATIHVANNGLDTATCGSRNAP
                     CRSIVRGADRASAGDTVLVAPGKYGDLNGDGLLGGPGEEVSSGPAAVRIGKPLRVVSS
                     AGAALTVIDAGGGVWWTVDIDADGVTFGIAGGGFTMVGGQFEGLNCDGQTNVRIGGNI
                     ASGSPAHGFVVYSNGTIEMSDNIAHGNTGAGIIIGSSLETSRVIVRNNQSYSNGTGIV
                     SGTYGRHEIAYNEVSNNTGVGISVDHTPSFIHHNVVTGNDMGIASNSWSPDQPPSAGP
                     VLYRNSLIGNTRFGIMVSVGPPVRTVVKENNFFGNGVSPDEFGRNCGLANFTNETLNA
                     VNSYWGASSGPGAEPADVACGNNPVVTTPFARSPN"
     gene            3191..>3454
                     /locus_tag="VIV63_08040"
     CDS             3191..>3454
                     /locus_tag="VIV63_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124587.1"
                     /translation="MPEREVTWRDKLRYRFENTLSKGTIAIIGWLALLSTAIVMLAAF
                     VLAIMRVGQDPADHEHPYGFFEGMWQSMLRTLDPGVVAGDNGWQ"
BASE COUNT          684 a         1102 c         1078 g          590 t
ORIGIN      
        1 tggctccgtt ttgctccgca aaacggtgcc tgaccccatt cttcgtacca ctgccgcagg
       61 aaataacaaa tgaactacaa cgccgccctc tacggcttcg gcgcgctgct gctcggcgcc
      121 gtcggaattt actttcacga cttcgccatg cagtggcagc ccgtgcccgc cggtatcgcc
      181 ggccgcacag cacttgctta tgccagcgga gcactgctcg tcgtctgtgg cgcggcaatc
      241 ctcttgcgca ggtacgaacg catcggcgtg ttgctactgg cgtcgttcta ctggctgtgg
      301 atgctcgtgc tgaatctgcc gttggccatc aagtcgtaca cgcacatcgg cgcgtggaac
      361 agtcccgccg aaatcgcatt tctcgccgcc ggaggtactg cgctggtcgc gaccagcgcg
      421 ggccagcttc gcaacacgct caccctcgtg gcgcgcatcg tcgccggcgt gagcgccctg
      481 gtcttcggcc tcgcgcattt caactacgcg gaattcaccg cgagcatggt gccggcctgg
      541 attccctaca aacttttctg ggcctacgcc accggcgccg gccatttcgc ggcgggtctc
      601 gcgctggtga gcggcatccg cgcgcggctc gcggcgacgt tgttagccgg catgatggga
      661 tcgttcgtgg tgctgctgca tattccgcgc gtcatcgcat cgccggatca gcacgtcgag
      721 tggatcatgc tcgccatcgc gagctcgctg tccggcgcgg cgctgctggt caggaagtac
      781 gcaacgtaac ggcggccggt tcgccgccga acagccgctt gaacacgaag tgcgagggca
      841 ggaagacgag caggggcagc gcgagcatca tcgcgccgag ccacgccagt tccggcatgc
      901 gctgctgggg gccggccatg ttcaggccga acacgaagtt gatgttcacc gacagcgtcg
      961 gatcgtcggg cggcgccggc gggcgaggca ggaacagata gctcaccagc agcaaggccc
     1021 acgtgaccac agtccacgag acgagggcgc gcgggtcata cccgagccgc gacatcgcga
     1081 aaatgagcac gaacggcagc cacgcatgaa acagcgcgag gacttttttg aataccggca
     1141 ggttgtcgtt gagcatgaac gcagtcatgc gcactggaga aacctccgcc gcggtcagcg
     1201 ccagatccac gctccacagt agctggatca tcacgacgcc caccgccgcc atcgccaccg
     1261 gcaacgcacg ctcgagacag attccgatcg tgcccaggaa cagcgccacg ttgcagaagt
     1321 acagcaggct gtgcacgccc tgaaacaggg tgttgtaggc gatcagcgtg agcgaaaacg
     1381 ccgcaagaaa tatcttgaga ccgagcggta gcgtggatgt gttcttcatt gcggcgagcc
     1441 tttgcgctgt cgcgggcgcc aggcgcccat aaacagcgtt aacctacaat gcaaagacgc
     1501 tatccatgcg cgaacgaggt caggaaatgg cgaaaatctt gattcgcatc ccacaaaatg
     1561 ctctgtcacg aaaccatgag tgatgacaca gatagcccgg ccggaccgcc gatccaggtg
     1621 tcctacaggg atctgagcag cggtgcactg cagggggtca tcgagtcata cgtgcttcgc
     1681 gaaggcacgg attacggtga acgggacgtt ccgttcgaat cgaaggttgc ccaggtgcgg
     1741 cgccagctcg aaaccggcga ggccgccatc tacttcgatc cggaaaccga aagcatcgat
     1801 atcaaaccag ttacgggcgc gcgttccgcc cgttgaatgc gctgcccgat aggcgaatca
     1861 acgaccgcat gtaatagttc cgcccgcggc ggacggcccg cggaagttag aacgaacatc
     1921 aacaaggaca gggaaatcat gaagcacgtt gcaatcttca tcggcgccgc gttgtgcgcg
     1981 gccgcttcgc aggccatggc cgccggcgcg actatccacg tggccaacaa cgggctggac
     2041 acggccacct gcggcagccg caatgcgccg tgccgatcca tcgtccgcgg cgccgatcgc
     2101 gccagtgccg gcgatacggt gctggtggcg ccgggcaagt acggcgatct caatggcgac
     2161 ggcttgttag ggggtccggg cgaggaagtg tcatcgggcc cggctgccgt gcgaatcggc
     2221 aagccgctgc gcgtcgtttc gagcgcgggc gccgcgctga ccgtcatcga tgcgggcggc
     2281 ggcgtgtggt ggaccgtgga catcgatgcc gacggcgtga ccttcggtat cgcggggggt
     2341 ggattcacga tggtgggcgg gcagttcgaa ggcctcaact gcgatggcca gaccaacgtg
     2401 cgcatcggcg gaaacatcgc ctcgggctcg cccgcgcacg gcttcgtcgt ctactcgaat
     2461 ggcaccatcg aaatgagcga caacatcgcg catggcaaca ccggcgcggg aataataatc
     2521 ggtagttcgc tcgagaccag ccgcgtgatc gtgcgcaaca atcagagcta ttcgaacggc
     2581 accggcatcg tgtccgggac gtacggacgt cacgaaatcg cctacaacga agtgtcgaac
     2641 aacacaggtg tcggcatctc ggtggaccac actccgtcct tcatccacca caacgtcgtc
     2701 accggcaacg atatgggcat cgcttcgaac tcctggtcgc ccgaccagcc gccgagcgcg
     2761 ggccccgtcc tctatcgcaa ctcgctgatc ggcaatacgc gtttcggcat catggtttcc
     2821 gtgggtcccc cggtgcgcac cgtggtcaag gaaaacaact tcttcggcaa cggcgtcagc
     2881 cccgacgaat tcggcaggaa ctgcggcctc gcgaacttca cgaacgagac cctgaatgcc
     2941 gtgaacagct actggggtgc gtccagtggt cccggcgccg aaccggccga tgtcgcgtgc
     3001 ggcaacaacc ccgtcgtcac gacaccgttc gcccgatcgc ctaactgacg atttcgagtc
     3061 acacatggac tcgcctccgg ccggggcgag tccatcagta cctccgctaa caaacctcga
     3121 accgcggcgt ccacggttcg ctactggaag cagcgcaagt tagactctcg cccctaggga
     3181 ggcgaggttc atgccggaac gcgaagtgac gtggcgcgac aaattgcgct accggttcga
     3241 gaacacactt tcaaaaggca ccatagccat catcggctgg ctggcgctgc tgtccaccgc
     3301 catcgtgatg ctcgccgcgt tcgtgctggc gatcatgcgc gtagggcagg atccggccga
     3361 ccacgagcat ccatacgggt tcttcgaggg catgtggcag agcatgctgc gaacgctcga
     3421 cccgggcgtg gtggccggcg acaacggctg gcag
//
LOCUS       DATDEU010000338         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_352616, whole genome shotgun sequence.
ACCESSION   DATDEU010000338 DATDEU010000000
VERSION     DATDEU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352616"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VIV63_08045"
     CDS             <1..445
                     /locus_tag="VIV63_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6124588.1"
                     /translation="DAPVVGGLGGTFAGSPLACAAGLAVLEVLEKEKLNERAEQMGGK
                     IAARLRQMQAKFNCIGEVRSLGMMVAMELVKNRRADAPDAELTKALVQAAGRRGLILL
                     SCGIYSNVIRILAPLTIPEAQLEEGLGLLEQALAEATQGAATAVA"
     gene            490..873
                     /locus_tag="VIV63_08050"
     CDS             490..873
                     /locus_tag="VIV63_08050"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY6124589.1"
                     /translation="MAKASKPETVDEYIAAAPKPIRPRLREMHALIMASAPGATTSLK
                     WSKPAYSYRRILVVFGAFKRHIGFFITPAVKRAFEKELAGFKTASSSVQLPFDKPLPK
                     SLIRKIVRFRVKQERSQDAKWRVEI"
     gene            1031..1531
                     /locus_tag="VIV63_08055"
     CDS             1031..1531
                     /locus_tag="VIV63_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124590.1"
                     /translation="MKMTTISLGLAAALGATTAFAHGDHDPSQGHDKPPPFAILQLDQ
                     VRDATARFIDVAAAEAAGYHDIGLFVPNMGWHYMKDALVDAKFDPTKPELLVYADDPC
                     GGKRKLVAVEYAVPLSLSKRAPAGFVGRADEWDANQQFQLWTLHAWLFEFNGDGVFAP
                     FNPRLP"
     gene            complement(1606..1905)
                     /locus_tag="VIV63_08060"
     CDS             complement(1606..1905)
                     /locus_tag="VIV63_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124591.1"
                     /translation="MEPIADTFDAAFSKTVDLANKMADKDKDADLWDIADGMLAGAIQ
                     YWLYSRQPCGDTRCQDCMPISTAEGRMSELKRLIEQLASESEYFHTPTDSNAGRA"
     gene            complement(1986..>2792)
                     /locus_tag="VIV63_08065"
     CDS             complement(1986..>2792)
                     /locus_tag="VIV63_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124592.1"
                     /translation="ENAGHAPLFFMVTLGVLLTMREHPRFPGARLYALAGLIGVGAGF
                     LSEVIQKPLARDASWEDVVADTIGVVCALASYALFDRRLKLRTWQKLGVLAVSLSCIA
                     IFITPIVRMTRAYVHRNGQFPVLADFHSRIELYWTLSVGVNREIVNNALDVELVAEEF
                     PGVAFHEPVPDWRRFKILVIDVENPDTVPLNLGVRVHDRRHNRSFNDRFNRRFNLAAG
                     ERRTLRIPLEDIRNGPRLRLMDMAHISDITLFRGGPAGSRHLRVYSLRLE"
BASE COUNT          521 a          886 c          903 g          482 t
ORIGIN      
        1 ggacgcgccg gtggtcggcg gcttgggcgg cacgttcgcc ggttcgccgc ttgcctgcgc
       61 cgcggggctg gccgtgctcg aagtgctcga gaaagaaaaa ctgaatgaac gtgccgagca
      121 gatgggcgga aaaatcgcgg ctcgcctcag gcagatgcag gccaagttca actgcatcgg
      181 cgaagtgcgt tcgctcggca tgatggtggc gatggaactg gtgaagaacc gccgtgccga
      241 tgcgcccgac gccgagctca ccaaggccct ggtgcaggcc gcgggacgcc gcggactgat
      301 cctgctgtcc tgcggcatct actcgaacgt catccgcatc ctcgcgccgc tcaccattcc
      361 cgaggcgcag ctcgaggaag gtctggggct gctcgagcag gcgctcgccg aagcgaccca
      421 gggcgccgcc accgccgtcg cgtaaatggg gacactcccc atttcctagc aaaaggggcc
      481 ggacctgcca tggctaaggc gagcaagccc gagaccgtcg acgaatacat cgccgccgca
      541 cccaagccca tacgtccgcg attgcgcgag atgcatgcgc tcatcatggc ctcggctccc
      601 ggcgccacca cgtcgctgaa atggagcaag ccggcctatt cctaccggcg catcctcgtg
      661 gtcttcggcg cgttcaagcg ccacatcggg ttcttcatca cgcccgcggt gaaacgggcg
      721 ttcgagaaag agctcgcggg attcaagacc gcgagctcat cggtgcagct ccctttcgac
      781 aagccgctgc cgaaatcgct gatccggaag atcgtccgct ttcgcgtgaa gcaggagcga
      841 tcgcaagacg cgaagtggcg cgtcgagatt tagtcggaag acgatcgggc atgaacggcg
      901 gacgcgcgag catgagcgcg gacgacgggt catcgtcgcc ggacggccgg tcattcgcgg
      961 cgagatcctg aatggttagt ctgctcccgt ttcccggggg ctcgaccaga ctaactatca
     1021 ggagatgaca atgaagatga caacgatttc gctggggctc gccgccgccc tcggggcgac
     1081 caccgctttc gcgcatggcg atcacgatcc ctcgcaggga cacgacaagc cgccgccgtt
     1141 cgcgattctg cagctcgacc aggtgcgcga tgccaccgcg cgcttcatcg atgtcgcggc
     1201 ggccgaagcg gccgggtatc acgacatcgg cttgttcgtg ccgaacatgg gctggcacta
     1261 catgaaggac gcgctggtcg acgcgaagtt cgatcccacg aagccggagc tgctcgtgta
     1321 cgccgacgat ccgtgcggcg gcaagcgcaa gctggtggcg gtggagtacg ccgtgccgct
     1381 gtcactctcg aagcgcgcgc ctgccggttt cgtcggccgc gctgatgagt gggatgcgaa
     1441 tcagcagttc cagttatgga cgctgcatgc gtggctgttc gagttcaacg gggatggcgt
     1501 gttcgcgccg ttcaatccgc ggttgccgtg aaatgaaaag gggacgcgtt tgaggcgcgt
     1561 ccccttttgc tagcaaatca ggaatgtccc cattacgccc gctgactaag cccggccggc
     1621 gttcgaatcc gtcggcgtgt ggaagtactc gctctcggac gcgagctgct cgatgaggcg
     1681 cttcagttcc gacatgcggc cttcagcggt gctgatgggc atgcagtcct ggcagcgcgt
     1741 gtcgccgcaa ggctggcgcg aatacagcca gtactggatc gcaccggcga gcatgccatc
     1801 ggcgatgtcc cagagatccg cgtccttgtc cttgtccgcc atcttgttgg caagatccac
     1861 ggtcttggag aaggcagcat cgaaggtgtc ggcgatcggt tccatggggc gcagtatagg
     1921 cacccccacc cgtgcgcgaa aagcctcaat tgggcccgcg agcatcctat gttcaacgca
     1981 gcgcgttact ccagccgcaa agagtaaaca cgcaagtggc gcgatcccgc cggaccgccg
     2041 cgaaacagtg tgatgtccga gatatgcgcc atgtccatga gccgcagtcg aggcccattc
     2101 cgtatgtctt ccagcggtat ccgcagcgtg cgccgctcgc cggccgccag attgaagcgc
     2161 cggttgaagc ggtcgttgaa gctgcggttg tggcgcctgt cgtgcacgcg cacgccgaga
     2221 ttcagcggca ccgtatccgg attctccacg tcgatgacca gaatcttgaa acggcgccag
     2281 tccggcaccg gttcgtggaa cgccacgccg gggaattctt ccgcgaccag ttccacgtcg
     2341 agcgcgttgt tcacgatctc gcggttcacg ccgacgctca gcgtccagta gagctcgatg
     2401 cgcgagtgga agtcggcgag caccggaaac tgcccgttgc ggtgcacata ggcgcgcgtc
     2461 atcctaacaa tcggcgtgat gaatattgcg atgcaggaga gcgagacggc gagcacgccg
     2521 agcttctgcc acgtgcgcaa tttcaggcgc cgatcgaaca gcgcgtacga cgccagcgcg
     2581 cagaccacgc cgatggtgtc cgcgaccacg tcttcccacg acgcgtcacg cgccagcggc
     2641 ttctggatca cctcgctcag gaatcccgcg ccgactccga tcaggcccgc gagcgcatag
     2701 aggcgtgcac cgggaaagcg cggatgttcg cgcatggtga gcaggacacc gagcgtcacc
     2761 atgaagaaca gcggtgcgtg tcccgcgttc tc
//
LOCUS       DATDEU010000339         3818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_645886, whole genome shotgun sequence.
ACCESSION   DATDEU010000339 DATDEU010000000
VERSION     DATDEU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645886"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VIV63_08070"
     CDS             <1..1379
                     /locus_tag="VIV63_08070"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6124593.1"
                     /translation="AEIRAVRAGADPTNAAALLANEPLNDLLRNRVQAEAAVSSMETR
                     LTDNHPTLVKQREVLSSINQTLEKQLARVESEAAASVNSWQRQVNDLSGAVNSETSNK
                     ETQDKVSSALPALIAEAQVKRTVFETVLNRYQTQMAERGYSQPTAVIVSRAVPPAKPS
                     FPRTSLFLAIGLVASVLGGAFMAILVQLMRPGSPSLNSLADAVGLRSLVAIPRFRNES
                     REPGQVKIRDPRLYIESIRSLRHAIFELQSARETKVCLFTSVAPSQGKTLVAMSVARS
                     MARSGVRTLFLEMDLRCPAASKLARVGECTRGVAAVLEMRASINDVLQVDESTGLDML
                     LAEKNASQALDKLTVQAFSGLMARLRQRYDAIIVDSPPAGLLSDALTLACVADQTVIV
                     ARNGETSNSELATTTRLLRERGATLAGLVLTHVDPRDMAFAEKSMSRYVLGLTPRLTA
                     VPTIQRAS"
     gene            complement(1384..2457)
                     /locus_tag="VIV63_08075"
     CDS             complement(1384..2457)
                     /locus_tag="VIV63_08075"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEY6124594.1"
                     /translation="MAAFLGGTAHAASAAPAVAMDAAAASRFAELALKCVHQEYPNKI
                     AHVIATDADVRPPRELYPAFYGCYDWHSAVHGHWLLVRLMERFPDAAFVPRARAALAR
                     SLTKENIAVEAAYLQRGGRASFERPYGLAWLLQLSAELRAWKDPQSQEWAKHLAPLEA
                     ESAARIKRWLPDLYYPIRVGEHDQTAFSFGLIWDWANVAGDAEMRRLLTDAAQRFYIT
                     NRNCPLTYEPSGQDFLSPCLAEADFMRRVLEPATFSKWLKTFLPVIPTNPRTEWLTPA
                     IVTNRSDPKLAHLDGLNLSRAWMLEGILHGLPEGDRRIPALADAAKRHRDAALPQVTG
                     EHYEGGHWLGTFAVYLVTGPGNW"
     gene            complement(2481..3650)
                     /locus_tag="VIV63_08080"
     CDS             complement(2481..3650)
                     /locus_tag="VIV63_08080"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY6124595.1"
                     /translation="MSQQAGLLRSREGHARVTYVELFFDLVFVFAVTQLSHSLVAHLT
                     LGGALQTLFLLLAVWWVWMYTCWFTNWIDPDKPAVRMLLFTLMLAGLLMSAAIPNAFG
                     HEGLLFAISYAFIQVTRSVFMCVATRGRDEVNHRNFLRITAWLMLSAVFWIAGGLAEG
                     YERVAAWCIGLSLEIGGPFIGYFTPGLGRSNTTDWKVDGAHMAERCALFVIIALGESI
                     LVTGATAASLPATTAAVCAFLVAFMGSVAMWWIYFNIGAERNSRQIAGSADPGRFART
                     VYTYVHIPIVAGIVVCAVADEITIAHPGGHLELPAAIALLGGPALYLLGNVFFKRASA
                     KYYPLSHLVGLGLLAGIAPFALLFTPLALGAATTAVLITVAVWETRSFANRGHPA"
BASE COUNT          707 a         1279 c         1259 g          573 t
ORIGIN      
        1 tcgcggagat ccgcgcagtg cgcgccggcg ccgacccgac caacgcggcc gccctgctcg
       61 cgaacgaacc cctcaacgac ctgctgcgca accgcgtgca ggccgaggcc gcggtgagct
      121 cgatggaaac gcggctgacc gacaaccatc cgacgctggt caagcaacgc gaagtcctgt
      181 ccagcatcaa ccagacgctg gaaaagcagc tcgcgcgcgt cgagagcgaa gcggccgcca
      241 gcgtgaactc ctggcagcgc caggtgaacg acctgtcggg cgcggtcaac agcgagacca
      301 gcaacaagga aacgcaggac aaggtgtcgt cggcactgcc ggcgctcatc gccgaagcac
      361 aggtgaaacg cacggtgttc gagaccgtgc tcaatcgcta ccagacccag atggccgagc
      421 gcggctattc gcagcccacc gcggtcatcg tgtcgcgcgc ggtgccaccc gccaaaccgt
      481 cgttcccgcg aacgtcgctg ttcctcgcca tcggcctggt ggcgtcggtg ctcggcggcg
      541 cgttcatggc catcctcgtg cagctcatgc gaccgggttc gcccagcctc aactcgctgg
      601 ccgacgccgt cggcctgcgc tcgctggtcg ccattccgcg cttccgcaat gaatcgcgcg
      661 aaccgggcca ggtgaagatc cgcgacccgc gtctctatat cgagagcatc cgctcgctgc
      721 gtcacgcgat cttcgaactg cagtccgcgc gcgagaccaa ggtgtgcctg ttcacgtcgg
      781 tagcgccgag ccagggcaag acgctggtgg ccatgtcggt cgcgcgatcc atggcgcgct
      841 cgggcgtgcg cacgctgttc ctcgagatgg atctgcgctg ccccgccgcc agcaaactgg
      901 cgcgcgtcgg ggagtgcacg cgcggcgtcg ccgcggtgct cgagatgcgc gcgtcgatca
      961 acgacgtcct gcaggtcgac gagagcaccg gtctcgacat gctgctcgcg gagaaaaacg
     1021 cgtcgcaggc gctcgacaag ctgacggtcc aggccttctc gggcctcatg gcccgcctgc
     1081 ggcagcggta cgacgcgatc atcgtcgaca gtccgccggc gggcttgctg tcggacgcgc
     1141 tcacgctcgc ctgcgtggcc gatcagaccg tgatcgtggc gcgcaacggc gagacgtcca
     1201 attcggagct cgcaacgacc actcggctgc tgcgcgaacg cggcgccacg ctcgcgggct
     1261 tggtgctcac gcacgtggat cctcgggaca tggcgttcgc ggagaagtcg atgtcgcgct
     1321 acgtgctcgg cttgacgccg cggctgaccg ccgttccgac gattcaacgc gcgagttagc
     1381 cgtctaccaa ttgcccgggc cggtcaccag gtagaccgcg aacgttccga gccaatgacc
     1441 gccttcatag tgctcgcccg tgacctgcgg aagcgcggcg tcgcggtggc gcttcgcggc
     1501 gtccgcgagc gccgggatac gtcgatcgcc ctcaggcagc ccgtgcagga ttccctccag
     1561 catccacgcc cggctcaggt tgagaccatc caggtgcgcg agcttgggat cgctgcgatt
     1621 cgtgacgatg gccggcgtca gccattcggt gcgcgggttc gtcgggatca cgggcagaaa
     1681 agtcttgagc catttcgaga atgttgccgg ctcgaggaca cggcgcatga agtcggcttc
     1741 ggccagacat ggcgacagga aatcctgacc tgaaggttca taagtcagcg gacaattccg
     1801 attggtgatg tagaagcgtt gcgcggcatc cgtcagcagt cgccgcatct ccgcatcgcc
     1861 ggctacattc gcccagtccc agatgaggcc gaatgaaaac gccgtctgat cgtgctcgcc
     1921 cacgcggatc gggtaataca gatccggcaa ccagcgcttg atgcgcgcgg ccgactccgc
     1981 ttcgagcggc gcgaggtgct tcgcccattc ctgcgactgc gggtctttcc acgcacgcag
     2041 ctccgccgaa agctgcagca gccacgcgag tccgtacggc cgctcgaacg aagcgcgacc
     2101 gccgcgctgc agataggccg cttcgaccgc gatgttttcc ttcgtgagac tgcgcgcgag
     2161 cgcggcgcgg gcgcgcggta cgaaggctgc gtccggaaaa cgctccatga gccgtacgag
     2221 cagccaatgg ccgtgcaccg ccgaatgcca gtcgtaacag ccgtagaacg cgggatacaa
     2281 ctcgcgcggt ggacggacgt cggcatccgt cgcgatgacg tgcgcgatct tgttcggata
     2341 ttcctgatgc acgcatttga gcgcgagctc ggcgaagcgc gacgcggccg cggcgtccat
     2401 ggccaccgcg ggcgcggccg aagcagcgtg agcggtgccg ccgagaaaag cggccacaac
     2461 tccgatcagc agaaagtgtt tcacgccgga tgccctcggt tcgcgaacga tcgtgtttcc
     2521 cagaccgcga cggtaatgag caccgccgtg gtcgcggcgc cgagcgccag cggggtaaac
     2581 agcagggcga acggcgcaat gccggccagc aggcccaggc ccaccaggtg cgagagcggg
     2641 tagtacttcg cactcgcgcg cttgaagaac acgttgccca gcaggtagag cgcaggtcca
     2701 cctaacaacg cgatggccgc gggcagctcc aggtgtccgc ccggatgtgc gatggtgatc
     2761 tcatccgcca cggcgcagac cacgatgccg gcgacgatcg ggatgtggac gtaggtgtac
     2821 acggtgcgcg cgaaccgccc cgggtccgcc gatcccgcga tctggcggct gttccgttcg
     2881 gcaccgatgt tgaagtagat ccaccacatc gccacgctgc ccatgaacgc gacgaggaat
     2941 gcgcagacgg ccgcggtcgt cgccggcagc gatgccgcgg tggcgccggt gaccaggatg
     3001 gattcaccga gcgcgatgat gacgaacaat gcacatcgct cggccatgtg cgcgccgtcc
     3061 accttccagt ccgtggtgtt cgagcgcccg aggcccggcg tgaaatagcc gatgaacggc
     3121 ccgccgattt cgagcgacaa cccgatgcac cacgccgcta ccctttcgta gccttcggca
     3181 agtccgccgg caatccagaa caccgccgac aacatcagcc acgccgtgat gcgcagaaag
     3241 ttgcggtggt tcacctcgtc gcgaccgcgc gtcgccacgc acatgaacac cgagcgagtg
     3301 acctggatga acgcgtacga gatcgcgaac agcagccctt cgtggccgaa cgcgttggga
     3361 atcgcggccg acatcagcag gcccgcgagc atcagcgtga acagcagcat gcggaccgcg
     3421 ggtttgtccg gatcgatcca gttggtgaac cagcaggtgt acatccatac ccaccacacg
     3481 gcgagcagca ggaacaatgt ctgcaacgct ccgccgagcg tcaggtgcgc gaccagcgag
     3541 tgcgagagct gggtcaccgc gaacacgaac acgaggtcga agaacaactc gacgtaggtg
     3601 acgcgcgcgt ggccttcgcg agaacgcagc aggccggctt gttggctcaa ggcgaggctc
     3661 cgaatgcggg cggcaacatc tctgcgaaac acgaggtgtc gagcgtgcgg gcggcggcgc
     3721 cgtcgagaaa gcggaccatc accgcgtcga ggcaggtgtc gagaccggtg aggccgtcga
     3781 gcacatgcgc gccgttcggc acgcgcacga ccaggccc
//
LOCUS       DATDEU010000340         2688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1375383, whole genome shotgun sequence.
ACCESSION   DATDEU010000340 DATDEU010000000
VERSION     DATDEU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375383"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..458
                     /gene="nrdR"
                     /locus_tag="VIV63_08085"
                     /pseudo
     CDS             18..458
                     /gene="nrdR"
                     /locus_tag="VIV63_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
     gene            548..1657
                     /gene="ribD"
                     /locus_tag="VIV63_08090"
     CDS             548..1657
                     /gene="ribD"
                     /locus_tag="VIV63_08090"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY6124596.1"
                     /translation="MSFSAFDEAAMRRALELAERGLYSTHPNPRVGAVLARDEAIVGE
                     GWHERAGEAHAEPIAIRAAGERARGATAYVTLEPCSHHGRTPPCVDVLLAAGVRRVVY
                     AVGDPNPRVNGAGAARLKEAGVIVEAGLLSSESEALNEGFLMRMRQRRPFVRLKSGAS
                     LDGRVALANGKSKWITSEAARADVQHWRARSGAVLTSAATVLADDPRLDVRIEAPRQP
                     LRVVLDRRRTLRKSARVLAPPGDALVFSAPTATRKAGPGDEFLGAARLERMRVKRSHL
                     DLTAVFARLAELEINDVLVEAGPGLSGALLAAGLVDEWLLYVAPKLLGKDAKPIASFA
                     RLTRLEAAPEFALLESKAVGPDLRLRLRPLAREPG"
     gene            1658..2266
                     /locus_tag="VIV63_08095"
     CDS             1658..2266
                     /locus_tag="VIV63_08095"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY6124597.1"
                     /translation="MFTGIVQEIGAIRRIETRSAAGASEDQRIEVSFAAIARDRLNLG
                     DSISVDGVCLTVAALNAFSFHADVSGETLRVTTLGAKPEGSPVNLEPALRAGDSLGGH
                     WVSGHVDGIADVLATANDARSLRVEFAAPHALARYIARKGSVTLDGVSLTVNEVDGVK
                     FSVNLIPHTLEVTTLGRLAAGARVNLEIDTLARYVERLSEYK"
     gene            2263..>2688
                     /locus_tag="VIV63_08100"
     CDS             2263..>2688
                     /locus_tag="VIV63_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY6124598.1"
                     /translation="MKPLNTIEEILADIAAGRMVVIMDDEDRENEGDLIMAAQKVRAD
                     DINFMARFGRGLICLTLTAERCRQLRLPLMVSDTHREHRTNFTLSIEAAEGITTGISA
                     HDRAQTVIAAVRPDARPEDLRQPGHIFPVMAQPGGVLTRA"
BASE COUNT          481 a          889 c          906 g          412 t
ORIGIN      
        1 cataacgagg gcgtgccatg cactgtccct tctgcgcgca cgacgacacc aaggtaaacg
       61 actcgcggct ggcggccgaa ggcacgcaga ttcgccggcg ccgcgagtgc ctggcctgcg
      121 gcgaacgatt caccacgttt gaaacagcgg agctggtcat gccgctggtg gtgaagaacg
      181 atcagcgccg cgtgccgttc gacgagaaca agttgcgcgg tggcctgcac aaggcgctcg
      241 agaaacgccc ggtgagccgc gaatcggtgg aagaagccgt cgggcacatc acgcgaaagc
      301 tgcgcagcct gggcgaacgt gaagtcacct cgcgccagat cggcgaaatc gtgatggaag
      361 agctgcacaa gctcgatgaa gtggcctatg tgcggtacgc gtcggtctat cgtcatttcc
      421 aggacgtcga agcctttcgc gacgaggtgg accggctgcg gcaccggcgc ggcaaggcgc
      481 cgagcgaagg ccagctatcg ttgctacccg gcgacctgtc gccgaagaaa accggcaagg
      541 agcgcaagtg agtttcagcg cattcgatga agccgccatg cgccgcgcgc tagagctcgc
      601 ggagcgtggc ctgtacagca cgcaccccaa tccgcgcgtc ggcgcggtgc tggcgcgaga
      661 cgaagcgatc gtcggcgaag gctggcatga acgcgccggc gaggcgcacg ccgagcccat
      721 cgccatacgc gcggcgggcg aacgtgcgcg cggcgccacg gcctacgtca cgctcgagcc
      781 ctgcagccac catgggcgca cgccgccctg cgtggacgtg ttgctggccg cgggcgtgcg
      841 gcgtgtggtg tacgcggtgg gcgacccgaa tccgcgtgtc aacggcgccg gcgcggcgcg
      901 cctcaaggaa gcgggcgtca tcgtcgaagc cggactgctg tcgtcggagt ccgaagcgct
      961 caatgaaggt ttcctgatgc gcatgcggca acgccggccg ttcgtgcgcc tgaagtcggg
     1021 cgccagtctc gacggacgcg tcgcgctcgc caacggcaag agcaaatgga tcacgagcga
     1081 agcagcgcgc gccgacgttc aacactggcg tgcgcgaagc ggcgcggtgc tcaccagcgc
     1141 cgccacggta ctcgcggacg atccacggct cgacgtgcgc atcgaagcgc cgcgtcagcc
     1201 gctgcgcgtg gtgctcgacc ggcgccggac tttgcgcaag tcggcgcgag tgctcgcgcc
     1261 acccggcgac gcactggtgt tttccgcgcc gacggccacg cgcaaggcag gccctggcga
     1321 tgaattcctg ggtgccgcgc gcctcgaacg catgcgagtg aagcgatcgc atctggatct
     1381 gacggcggtg ttcgcgcgcc tggccgagct cgagatcaac gacgtactgg tggaggccgg
     1441 gccggggctg tccggagcgc tgctcgcggc cgggctcgtg gacgagtggt tgttgtacgt
     1501 cgcgcccaaa ttgttaggca aggatgcgaa acccattgcc tcgttcgcga gactcaccag
     1561 actcgaggcc gcgcctgaat tcgcgctgct cgaatcgaag gccgtgggac ccgacctgcg
     1621 gcttcgtttg cgtccgctcg ccagggaacc aggataaatg ttcaccggta tcgtccagga
     1681 gatcggcgcc atccggcgca tcgagacgcg atcagccgcg ggcgcctccg aggaccagcg
     1741 catcgaggtg tcgttcgccg ccatcgcgcg cgaccggctc aacctgggag acagcatcag
     1801 cgtggacggc gtctgtctca ccgtcgccgc cctgaacgcg ttcagttttc atgccgatgt
     1861 gtcgggcgaa actttgcggg tcaccacgct cggcgcgaaa cccgaaggtt cgcccgtcaa
     1921 tctcgagccg gcgctccggg ccggcgacag tctcggtggt cattgggtgt ccggccacgt
     1981 cgacggcatc gccgacgtac tcgccaccgc gaacgacgcg cggtcgctgc gcgtggaatt
     2041 cgcggcgccc cacgcgctgg cacgttacat cgcgcgcaag gggtcggtca cgctcgacgg
     2101 cgtgagcctc acggtcaacg aagtggatgg cgtgaaattc tcggtcaatc tcatcccgca
     2161 cacgctcgaa gtcaccacgc tcggcaggct tgccgcgggc gcgcgcgtga atctcgaaat
     2221 cgacaccctg gctcgctacg ttgagcggct atcggaatac aaatgaaacc gctcaacacg
     2281 atcgaagaaa ttctcgccga catcgccgcc gggcgcatgg tcgtcatcat ggacgacgaa
     2341 gatcgcgaga acgaaggcga cctcatcatg gcggcgcaga aggtgcgcgc cgatgacatc
     2401 aacttcatgg cgcgtttcgg ccgcggcctc atctgcctga cgctgaccgc cgaacgatgc
     2461 cgccagctgc gcttgccgct catggtcagc gatacgcatc gcgaacatcg caccaacttc
     2521 acgttgtcca tcgaggccgc cgaaggcatc accaccggca tttccgcgca cgatcgcgcg
     2581 cagaccgtga tcgccgcggt gcggccggat gcgcgccccg aagatctgcg tcagccagga
     2641 catatcttcc ccgtgatggc acagccgggc ggcgtgctca cgcgcgcc
//
LOCUS       DATDEU010000341         2509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1229400, whole genome shotgun sequence.
ACCESSION   DATDEU010000341 DATDEU010000000
VERSION     DATDEU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229400"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..890)
                     /locus_tag="VIV63_08105"
     CDS             complement(171..890)
                     /locus_tag="VIV63_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY6124599.1"
                     /translation="MIAFTTAGLALAGAARADGVQLLSGSIGGTTDFVFRGLSLTRGK
                     PAAQVSLDLEFPREYYAGAFVATADPNVGPSPPVEIDFWAGRYWRMSENFSGDLRLSQ
                     YTYPDDPRRVSYNRTELTATLGFHNRFYFAAIYSPNTKAVGSSSGYEEGNVWAVELSG
                     RHPFNERLSLAAGVGHYGLDEVYHDSYNYWNVTLIATLAPFELQLAYLGVDGEAAEHF
                     VEDSVENRVALTALWRFSSNH"
     gene            1087..>2509
                     /locus_tag="VIV63_08110"
     CDS             1087..>2509
                     /locus_tag="VIV63_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY6124600.1"
                     /translation="MNALIARLAARWARPLFAIVVIYSAVCVLFMFTGWGGEAVTDYI
                     GAWSSFPVSIVICLVLWPSLSGASLTPLRRRAWRLIFVALVLDLIASVGWGYSALTEN
                     VTFGSWPDVLYIFYYPLAAWAFVLLYIDLGGRIRTPRALIDFTTVAIGFGSLLWFTAL
                     APVADMSAAELMDNWSVVGYGIGDAIAIITGAMVAMQITDWRAERPIVWLLVAMVATL
                     VSDLLWVNAELRGAYVIGGASEIGYSVFYLCMFAAVLYQLGHRQAIASTLGLQGDLRG
                     SLPIFALMVGAIALLDDRLHLASGQSPVLIWVMIAATLLVLAGQAFATREVVSLHREV
                     ATRRFDERLTELVRRSSDMIAICDADGVIRYASPSSEQLLGVAPGELAGQRLDDVLGP
                     EAPHVRQVFNEVIRAAHSEQTANFAIPQTDGEKRSYKMVIANLCHVDSIRGVTLNIRD
                     ISDATRLHDQLRTLAFHDSLTLLA"
BASE COUNT          389 a          785 c          840 g          495 t
ORIGIN      
        1 tggtccttcg accgggcgat gcgttccagc agttcgcgtt cggaggcgtc cgggccgggg
       61 ctgacggccc tcggtccttg agtttcaacg cttttgagtg ccacggcagc ggaccttgat
      121 cgacttactg ctcatgagta ccgcggatcg ggaaccgggt tcccggcgga tcagtggttg
      181 gacgaaaagc gccacagggc ggtcagggcc acccggttct ccaccgaatc ttcgacgaag
      241 tgttcggcgg cttcgccatc gacacccagg taggcgagct gcagctcgaa gggggccagg
      301 gtggcgatga gcgtgacgtt ccagtagttg taactgtcgt ggtagacctc gtccagaccg
      361 tagtggccga cgccggccgc cagtgacagg cgctcgttga acgggtggcg gccggagagt
      421 tccacggccc agacgttgcc ttcctcgtag ccggacgacg agcccacggc cttggtgttg
      481 ggagagtaga tagccgcgaa atagaagcgg ttgtggaagc ccagcgtggc ggtgagttcg
      541 gtgcggttgt agctcacgcg ccgcgggtcg tcgggatagg tgtactgcga aagtcgtaga
      601 tcgccggaaa aattctccga catgcgccag tagcggccgg cccagaaatc gatttccacg
      661 ggcgggctgg ggccgacgtt ggggtcggcc gtggccacga acgcgcccgc gtagtactcc
      721 cgcgggaatt cgaggtcgag tgagacctgc gccgcgggct tgccgcgcgt gagcgacagg
      781 ccgcggaata cgaaatcggt ggttccgccg atgctgccgc tcagcaattg cacgccatcc
      841 gcgcgcgccg caccggcaag tgcaagcccg gctgttgtga acgcaatcac aatcagcgtt
      901 tgccagggtc gcgtcacagc agggtttctc tcaaggcctt ccttatgccg cgggcagtct
      961 gccgagtcgt tcccgtgctg gcaaccattt ccagccgaaa agcctgcgta tcagcgctcg
     1021 tgtgccgatg tcttgtgact gcgatcagcg cgaatgcggc gctgatttgg gagcatcgac
     1081 gatccgatga atgcacttat tgccagactt gcagcccgct gggcgcggcc gttgttcgcg
     1141 atcgtggtga tctacagtgc tgtctgcgtg ctgttcatgt tcacgggatg gggcggcgaa
     1201 gccgtcacgg actacatcgg cgcatggtcc tcgttccccg tgagcatcgt gatctgcctg
     1261 gtgctgtggc cgtcgctgtc cggtgcgtcg ctgactccgc tgagaaggcg cgcgtggcgt
     1321 ctcatcttcg tcgcgctggt gctcgacctc atcgcgagcg tcggctgggg atacagcgcg
     1381 ctcaccgaga acgtcacctt cggctcgtgg ccggacgtgc tgtacatttt ttactatcca
     1441 ctcgcggcgt gggcattcgt gctcctgtac atcgatctcg gtggccgcat ccgcacaccg
     1501 cgcgcgctca tcgatttcac cacggtcgcg atcggtttcg gctcgctgct gtggttcacc
     1561 gcgctcgcgc cggtcgccga catgtcggcc gcggagctca tggataactg gtcggtcgtc
     1621 ggttacggaa ttggtgatgc gattgccatc atcaccggcg ccatggtcgc catgcagatc
     1681 accgactggc gcgccgagcg gccgatcgtg tggctgctcg tcgccatggt ggccacgctg
     1741 gtctccgacc tgctgtgggt caacgccgaa ctgcgcggcg cgtacgtgat cggcggcgcg
     1801 agcgagatcg gctattccgt gttctatctc tgcatgttcg cggcggtgct ctaccagctg
     1861 gggcatcgcc aggcgatcgc atcgacactg gggctgcagg gcgatctgcg cggatcgttg
     1921 cccatcttcg cgctcatggt cggcgccatc gcgctgctcg atgaccgtct gcacctggcg
     1981 agcggacagt cgcccgtgct gatctgggtg atgatcgccg ccacgctgct ggtgctggcg
     2041 gggcaggcct tcgcgacgcg cgaggtcgtg agtctgcatc gcgaagtggc cacgcgccgg
     2101 ttcgacgagc ggctcacgga gctcgtgcgc cgctcgagcg acatgatcgc catctgcgat
     2161 gccgatggcg tcatccgcta cgccagtccg tcgtcggagc agttgttagg tgtggccccc
     2221 ggggagctcg ccggccagag actcgacgac gtgctcgggc cggaagcgcc gcacgtgcgc
     2281 caggtcttca acgaagtgat tcgcgccgcg cattccgagc agaccgccaa tttcgcgatc
     2341 ccgcagacgg acggcgagaa acgttcctac aagatggtca tcgcgaacct ctgccacgtg
     2401 gactccatcc gcggcgtgac gctgaacatc cgcgatatct ccgacgcgac gcggctgcac
     2461 gaccagctgc gcacgctcgc gtttcacgat tccctcacgc tgctcgcca
//
LOCUS       DATDEU010000342        11622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_62456, whole genome shotgun sequence.
ACCESSION   DATDEU010000342 DATDEU010000000
VERSION     DATDEU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62456"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="VIV63_08115"
     CDS             complement(<1..1564)
                     /locus_tag="VIV63_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY6124601.1"
                     /translation="MTVALALLAGALMGLFSASLPANSWLLTAGISALLVFATRLRKV
                     AMACHLATLLVGMVLGWVSTLQWLSLKVPAASGDERVFIQGVIRGVPAREGPELRFDV
                     DAIIIEGRAASDARSRRARLVWRHPAVEPRPGESWRLLVRLAPPGEVRNFSGPDTARF
                     AFRDGVHFAGRVLPSALNERVALANSSVDTLRSRVAARIGEGVADPDAAALLTALAVG
                     LTDRLSADQWRVFNATGTTHLVAISGLHVTLFALVAFVAARHAWRWLPFARRVEREPF
                     AMLLGLAAAGGYALLSGFSVPAQRTWLMLAIFASVRLCARHAGAGRVWSLALVAVLCM
                     DPRAPLSAGFWLSFVAVGIILMTAAASAGLANVVRLQFAMLLALAPLTFAVFDALSLT
                     GFVVNLVAIPLVSFVLVPLVLAGAIAALLAPGLCHLFFGLAALLYEGFWPALTWSADL
                     DVSLWRSTPPFWWFPVGVLAALVAVRPWPLPLRGSAICAILPLLFAPSRMPEASPARI
                     EVLDAGRGAATLV"
     gene            1598..2116
                     /locus_tag="VIV63_08120"
     CDS             1598..2116
                     /locus_tag="VIV63_08120"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY6124602.1"
                     /translation="MPRRLFRKITPRSETLRTHWALKPFKRFFGDPRLWSLQRRTVTP
                     AFGAGLAICFVPLPIHIPIAAIVAIFCRIHVPTIMLTLMLMNPLTFLPLFLLAYKVGA
                     IATGAPPSNFHFEMSWDWVQNGLGANWKPFLVGCGLTGALFGLVGWAVLDLLWRYNVR
                     KRYRERAGSTSD"
     gene            complement(2117..2812)
                     /gene="lolD"
                     /locus_tag="VIV63_08125"
     CDS             complement(2117..2812)
                     /gene="lolD"
                     /locus_tag="VIV63_08125"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEY6124603.1"
                     /translation="MNEKVAEMVMEATGVERAFRQGPIDLQVLQGVNLAVRAGERLAI
                     IGASGSGKTTLLQILGGLDRPTAGIVKIAGSDIHALDEKARGELRNRTLGFIYQFHHL
                     LPEFSALENVAMPLLVRRVPRQEAENTARAILERVGLGERLIHRPHELSGGERQRAAV
                     ARALVTNPRIVLADEPTGNLDGGNAESVFELMLQLNRELATSLIVVTHDRRLAARMDR
                     ILELERGNLLERG"
     gene            complement(2805..4055)
                     /locus_tag="VIV63_08130"
     CDS             complement(2805..4055)
                     /locus_tag="VIV63_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY6124604.1"
                     /translation="MMYEWLIGTRYLRSTHRSGFVSFVASMSVIGLALGVAVLIVVLS
                     VLNGFERELRSRMLTVTSHATITGIDGEMANWRQAQTDAAREPGVVAVVPYVESRGLL
                     ANGERVAGAMVRGILPEEEGKAVGLGGRMKEGALSDLQPGKYQVILGSALATELALKV
                     GQSVVLMTPEGSATPAGFAPRMRRFTVIGIFESGMYEFDRGLALTHMADAAKLYRLGD
                     RVTGLRLALEDPFLAPLMVRTVARAIDYDGNGYFVNDWTRDHANFFRSIELTKSMMFF
                     ILLILVVVAAINLVATLVMIVKEKQTDIAILRTIGAAPANVLKIFLVQGALIGLVGTF
                     AGACLGWVLALNVTEVIHAIEKAFGIQFLDASVYLMSDLPSEVRLGDVLQVSGVALAL
                     SALATIYPAWRASRTLPAEALRHE"
     gene            complement(4052..5302)
                     /locus_tag="VIV63_08135"
     CDS             complement(4052..5302)
                     /locus_tag="VIV63_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY6124605.1"
                     /translation="MLNSLPLFVGLRYVRARSHKFFVSFITWVSLLCVCLGVTALIVI
                     LSVMNGLEGELRDRLLALSAHARVFVPPSATTAPDWNRLAEIVRRAPHVTGAAPFIEI
                     EALAVRKPEMLPVRLRGIDPSHEGEVDRVAKALVEGNLADLKPGSDHVIVGRGIAQML
                     GLGVGDSISVLVPTTDANGVPEPRLREFLVAGVFDAAVQDYDGELLIAAIEDVRALLP
                     NPDARMSLHVNFADALTASENSAALAKLLPADVTVRDWTVDHASYFRAIRIEKTMVAI
                     ILMLIVAVAAFYLVAMLAMVVTDKRTDIAILRTLGTSPRRVMAIFLIQGSVIAWFGVA
                     LGVLLGCWLGYYAGAAAAFVERLFRFEIFSSDVYMVTRIPSELHLEQIVWIAGIAMLI
                     TLAATIYPAYRASRVPPADALRYE"
     gene            5445..5732
                     /locus_tag="VIV63_08140"
     CDS             5445..5732
                     /locus_tag="VIV63_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124606.1"
                     /translation="MLARTMKMTVVALALGAAVPTFAETLAIEGQVAIKPAGIETPQR
                     GSSMTAVEQKFGAPANKAGPVGNPAITKWFYPNFVVVFENDKVLHAVVVAS"
     gene            complement(5772..6422)
                     /locus_tag="VIV63_08145"
     CDS             complement(5772..6422)
                     /locus_tag="VIV63_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124607.1"
                     /translation="MFRRLLSLLVVASPLAMAAGEPEAVEPLPDCIDAVVLARFLDYR
                     PGPIGPLEDDQIVMSWTWDVDVRVREVYFGDIDTGRLTIAAVLHTRFDDELRQPVLFL
                     TRKSDTWNLAYIEFAARDASGNRVLPIFEAPNERYEFSPQGWKPHDYAKWLAPVRYKG
                     SDVAAFEDVAEEETPAEDEWLRVAGGRKIAKRGFRVEDIPAMLAERRAVECARANP"
     gene            6483..9983
                     /gene="mfd"
                     /locus_tag="VIV63_08150"
     CDS             6483..9983
                     /gene="mfd"
                     /locus_tag="VIV63_08150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007966.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY6124608.1"
                     /translation="MPSLLHPPIPAPQARQRWHQLYGSARSLALAEAVESDNRPYLVI
                     ARDARELDQLRSELAFFLGGSRAIHVLPDWEVLPYDLFSPHPDIISERLAALAELPRL
                     KSAVLLAAADTLGQRIAPRGYVDGRTFNLAIGDKLPIEPLRARLVESGYASVSQVSAP
                     GEFALRGSLFDVYPMGASTPLRVDLFDDEIEVIREFDPETQRSGESLKQVRLLPGREM
                     PLDADSVKAFRLRYRKRFEGDPTKSVIYRGVSDGLAPPGIEFYLPLFFDTTDTLLDYL
                     PKDTVVCAGAEVPAAVDRAARSIAERFEERRHDVERPVLAPDELYLPPEELQARLDTF
                     TRVDLESFKIEDETKGFNFATAAPQEWRVDLRSERPLAPLENFLDSFPGRVLLAADSA
                     GRREVLLEMLRKHGLKPVGVAGWHEFVDSKAAFALCIAPEMAGLNILTPPIAVLGEAQ
                     LFGQRARQERRRRRAQSDPQAILRDLTALGPGSPVVHEEYGVGRYLGLQVMQVAGQDG
                     EFVVLEYAGGDKLYVPVQQLHLVSRYSGAAPESAPLHKLGTDQWARARKRAADKIRDV
                     AAELLDLYAQRQAKRGPSLPQRELEYQAFASSFPFEETADQAEAIRQVLQDLASEKPM
                     DRVVCGDVGFGKTEVAMRAAFVAAQAGKQVAVLVPTTLLAEQHTNNFRDRFADWPVRI
                     ESLSRFRTGKESNTVLEGIERGTVDIVIATQKLLHANVRFKDLGLIIVDEEHRFGVRD
                     KEKLKTLRAEVHVLTLTATPIPRTLNMALGGLRDLSLITTPPAERLAIKTFVTEWHAP
                     TLREAALREFRRGGQVYFVHNVVETIEKTAQEIARLIPEAEVRVGHGQMRERDLEQLM
                     VDFYHRRFNFLVCTTIIESGIDVPTANTIMIDRADRLGLAQLHQLRGRVGRSHHRAYA
                     YLLTPPRKALSLDAVKRLEAIESLEDLGAGFVLATHDLEIRGAGELLGESQSGELTEI
                     GLTMYLDMLEQAVKALKEGREPALDRPLYATAEVELRLPALLPESFVADVPVRLALYK
                     RLAAAPDNAAIDGLTEEIVDRFGPLPPPATNLLRVARLKLAARSIGIRRLDLGPQGGY
                     ALFEEQNQVDPRAVIRLVQHPDRDYRLEGPLKLRISRENEEDTERFEFAESFLAELKG
                     GQKQPDPKKPAAKPPAPGKR"
     gene            10006..10614
                     /locus_tag="VIV63_08155"
     CDS             10006..10614
                     /locus_tag="VIV63_08155"
                     /inference="COORDINATES: protein motif:HMM:NF022425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HEY6124609.1"
                     /translation="MTTTVRTLLLALILGAALPPASAQTAPNARVYTIELIVFRNMSG
                     QGGPEDWSVKPVARGPDTPDAPVTGKFVQTVPASQFQLNEVARKLQNTANYQPIAHFA
                     WQQTASSWGSRAGFTVAKLAGSAPGLSGIIYLESGTYLHLGMSLNYQTSNPPSGLAAA
                     PGTVFTLSEARRIRVDKPSYYDHPAFGVVALVTPANRSTGGR"
     gene            10737..>11622
                     /locus_tag="VIV63_08160"
     CDS             10737..>11622
                     /locus_tag="VIV63_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEY6124610.1"
                     /translation="MGMSRRDFTLLMSSATAALVTPPLLAAPLKVRRGIHTLSSAELD
                     VFRAGISEMKALPTEDFRSWMYQAGVHGSSVAEQAAIPDGATYWRQCKHGSPHFLTWH
                     RWYLLFCEEILRLLADDCHFTMPYWDYIADNFLPEPLRLPADASNSLYDGTRNGLVNN
                     GTNGISGLHTDALDELDFDTFSNDLASNPHNTVHNQIVGNMGAVATAGRDPVFWLHHC
                     NVDRYWECWIRQGGGRTHPGSPWSDEEFPFHSLTGRRDVIVADGLRTAHLGYTYDNLP
                     CGRRVIWELPDWIKTLKFE"
BASE COUNT         2116 a         3991 c         3705 g         1810 t
ORIGIN      
        1 acaccagcgt tgccgcgccc cgccctgcat cgagcacctc gatgcgcgcc ggcgaagctt
       61 ccggcatgcg cgaaggcgcg aacaacagcg gcagaatcgc gcagatcgcg gacccgcgaa
      121 gcggcagcgg ccagggtcgc accgcgacca gcgccgccag aactccgacc ggaaaccacc
      181 agaacggcgg cgtagaccgc cacagcgaga cgtcgagatc ggctgaccat gtcaacgcgg
      241 gccagaaccc ttcgtagagc aacgccgcga gtccgaagaa caggtggcac aaccctggcg
      301 cgagcagtgc cgcgatggcg cccgccagca ccagcggcac cagcacgaac gagaccagcg
      361 gaatggccac gaggttcacg acgaatcccg tgagcgacag cgcgtcgaat acggcgaacg
      421 tcagcggcgc gagcgcgagc agcatggcga actggagccg gaccacgttg gcgagacccg
      481 ccgacgctgc cgcggtcatc aggatgattc ccaccgccac gaatgacagc cagaaaccgg
      541 cggacagcgg cgctcgtgga tccatgcaca acaccgccac cagcgccagc gaccacacgc
      601 ggccggcgcc ggcatggcgt gcgcagagcc gcactgacgc gaagatggcg agcatgagcc
      661 acgtgcgctg cgcgggcacc gagaaaccgg acaacaacgc gtagcccccc gcggccgcga
      721 ggcctaacaa catcgcgaag ggttcacgct cgacgcggcg cgcgaacggc agccagcgcc
      781 acgcgtgacg cgcggcgacg aacgcgacca gtgcgaacag cgtgacgtgc agccccgaga
      841 tcgccacgag gtgcgtcgtt cccgtcgcgt tgaacacgcg ccattgatcg gcgctcagcc
      901 gatcggtgag tcccacggcc agtgccgtga gcaacgccgc cgcatcgggg tctgccaccc
      961 cctcgccgat acgcgctgcc acgcgcgagc gcagcgtgtc caccgagctg ttggccagcg
     1021 ccactctctc gttgagcgcc gatggcaaca ctcgtccggc gaagtgcacg ccatctcgaa
     1081 atgcgaatcg agcggtgtct ggcccggaga agttgcgcac ctcgccgggc ggcgcgagcc
     1141 ggaccagcag tcgccagctt tcacccggcc gcggctcgac agccggatgg cgccacacca
     1201 gccgcgcgcg gcgcgatcgt gcgtcgctcg ccgcgcggcc ttcgatgatg attgcgtcga
     1261 cgtcgaatcg cagctcgggc ccttcgcgcg ccggcactcc gcggatgact ccctggatga
     1321 acacacgctc atcgccagac gcggcaggca ccttgagcga aagccactgc agagtcgaca
     1381 cccaccctaa gaccattccc acgagcagcg tggcgagatg acacgccatc gcgaccttgc
     1441 gcaggcgcgt cgcgaatacg agcaatgcgc tgatcccggc cgtcagcaac caggaattcg
     1501 ccggcagcga cgcggagaaa agccccatca gagcgcccgc aagcagtgca agcgcgacgg
     1561 tcacgcgtgc aagcgcccga tgctagagtg cgccccgatg ccacgccgac tgttccgcaa
     1621 aataacgccc cgcagcgaaa cacttcgcac gcactgggcg ctcaaaccgt tcaagcgatt
     1681 cttcggcgac ccgaggttgt ggtcgctgca gcggcgcacg gtgacgccgg ccttcggcgc
     1741 gggactcgcc atctgcttcg taccgctgcc catccacatt ccgatcgcgg cgatcgtggc
     1801 gatcttctgc cgcattcacg ttcccaccat catgctgacg ctgatgttga tgaacccgct
     1861 gacgtttctg ccgctgtttc tgctggcata caaagtgggc gccattgcga ccggagcgcc
     1921 acccagcaat ttccatttcg agatgagctg ggactgggtg cagaacggcc tgggcgccaa
     1981 ttggaaaccc ttccttgtgg gttgtggact caccggcgcg ctgttcggcc tggtgggttg
     2041 ggcggtgctc gacctgctgt ggcgttacaa cgtgcgcaag agatatcgcg aacgcgcggg
     2101 atccaccagc gactaatcag ccgcgttcga gcagattccc gcgttccagt tcgaggatgc
     2161 gatccatgcg cgccgccagg cgtcggtcgt gcgtcacgac gatgaggctg gtcgccagct
     2221 cccgattcag ctggagcatg agctcgaaca cggattcggc gttgccaccg tcgagatttc
     2281 cggtgggctc atccgcgagc acgatgcgag gattggtgac gagcgcgcgc gcgaccgcgg
     2341 cccgctgacg ctcgccgccc gacaattcat gcggcctgtg gatcaaccgc tcgccgagtc
     2401 ccacgcgctc gagaatcgcc cgcgccgtgt tctccgcttc ctgtctcggc acccgtcgca
     2461 ccagcaacgg catcgccacg ttttccagtg ccgagaactc gggcaacagg tgatggaact
     2521 ggtagatgaa acccagcgtg cgattgcgca gttcgccgcg cgctttttcg tcgagcgcat
     2581 ggatgtcgct gccggcgatc ttcacgatac ccgcggtcgg ccgatcgagg ccgcccagga
     2641 tctgcagcaa cgtggtcttg ccggaacccg aagcgccgat gatggcgagg cgctcgccag
     2701 cgcgcaccgc caggttcacg ccctgcagca cctgcagatc gatgggtccc tggcggaacg
     2761 cgcgctcgac gccggtggct tccatcacca tttcagcgac cttctcattc atggcgcagg
     2821 gcctccgccg gaagcgtgcg cgatgcgcgc cacgcggggt agatagtagc cagcgccgac
     2881 agcgccagcg cgacgcccga cacttgcagt acgtcgccga ggcggacttc ggaagggagg
     2941 tcgctcatga gatacacact cgcatcgagg aattgaatgc cgaaagcctt ttcgatcgcg
     3001 tggatgactt cggtgacgtt gagggccagc acccagccga gacacgcacc ggcgaacgtg
     3061 ccgacgagac cgatcagcgc accctgcacc aggaagatct tcaacacatt ggccggcgcc
     3121 gcgccgatgg ttcgcaggat ggcgatgtcc gtctgctttt ctttcacgat catcacgagc
     3181 gtcgccacca ggttgatggc cgcgaccacc acgaggatga gcaggatgaa gaacatcatc
     3241 gacttcgtga gctcgatgga acggaagaaa ttcgcgtggt cgcgcgtcca gtcgttgacg
     3301 aagtatccat tgccgtcgta gtcgatggcg cgcgccacgg ttcgcaccat gagcggcgcg
     3361 aggaacggat cttcgagcgc gaggcgcagg ccggtcacgc gatctcccag ccggtagagc
     3421 ttcgcggcat cggccatgtg cgtgagggcg agcccgcgat cgaactcgta catcccggat
     3481 tcgaagatgc cgatcaccgt gaagcggcgc atgcgcggcg cgaagccggc cggcgtggcg
     3541 ctgccttcgg gcgtcatgag caccacgctc tgcccgactt tgagcgcaag ctcggtggcc
     3601 agtgcgctgc cgaggatcac ctggtatttc ccgggctgaa ggtccgacag tgcgccctct
     3661 ttcatgcggc cgccgaggcc gaccgccttg ccttcttctt cgggcaggat gccgcgcacc
     3721 atggctcccg ccacgcgctc gccattggcg agcagaccac gcgactccac gtacggcacg
     3781 accgccacga cgcccggttc gcgcgcggcg tctgtctggg cctggcgcca gttggccatc
     3841 tcgccgtcga tgcccgtgat cgtggcgtgc gaggtcacgg tcagcatgcg gctgcgcagc
     3901 tcgcgctcga agccgttgag caccgacagc acgacgatca gcaccgccac gcccagcgcg
     3961 agcccgatga ccgacatcga ggcgacgaac gacacgaatc cgctgcggtg cgtggaccgc
     4021 aggtaccgcg tgccgatgag ccattcgtac atcattcgta tcgcagcgcg tcggcgggtg
     4081 gaacgcgcga ggcgcggtac gccgggtaga tagtggcggc cagcgtgatg agcatggcga
     4141 tgccggcgat ccacacgatc tgctcgagat gcagctccga gggaatgcgc gtcaccatgt
     4201 acacatcgga actgaagatc tcgaaacgga acagccgttc cacgaatgcg gccgccgcac
     4261 ccgcgtagta gcccagccag caaccgagca gcacgccgag cgcgacaccg aaccaggcga
     4321 tgacgctgcc ctggatgagg aatatcgcca tgacgcggcg tggtgacgta cccagcgtgc
     4381 ggagaatcgc aatgtccgtg cgcttgtcgg tcacgaccat cgccagcatg gccacgagat
     4441 aaaacgccgc aaccgcgacg atcagcatga ggatgatcgc gaccatggtc ttctcgatgc
     4501 ggatggcgcg gaagtaactc gcgtgatcca ccgtccagtc gcggaccgtg acgtccgccg
     4561 gcagcaactt cgcgagcgcc gccgaattct cgctggccgt cagtgcgtcg gcgaagttca
     4621 cgtgcagcga catgcgcgca tcgggattcg gcagcagcgc ccgcacgtcc tcgatggcgg
     4681 cgatcagcaa ctcgccgtcg tagtcctgca ccgccgcgtc gaacacaccg gccaccagga
     4741 attcgcgcag gcggggttcc ggcacgccgt tcgcatccgt cgtcggcacc agcaccgaaa
     4801 tcgaatcgcc cacgcccagc cctaacatct gggcgatgcc gcggccgacg atgacgtgat
     4861 cggaacccgg cttgagatcg gcgagattgc cctcgacgag ggccttcgcc acgcggtcga
     4921 cttccccctc gtgactcggg tcgatgccgc gcagccgtac gggcagcatc tccggcttgc
     4981 gtaccgcgag ggcctcgatc tcgatgaacg gcgcggcgcc ggtgacatgg ggtgcccgcc
     5041 gcacgatctc ggcgagacgg ttccagtcgg gcgccgtggt cgccgaaggc ggcacgaaga
     5101 cgcgcgcgtg ggcggagagc gccagcaagc ggtcgcgcag ctccccttcc agcccgttca
     5161 tgaccgagag gatgacgatg agcgccgtca cgcccaggca cacgcaaagc agcgagaccc
     5221 aggtgatgaa cgagacgaag aacttgtggg accgggcccg cacgtagcgc agaccgacga
     5281 acaggggcag ggaattcagc atcgagcggg attctgggcc aaaactctga cgggcatcac
     5341 aaaagcgctg ttagtttggg tcggtacatg gcgacagggc tgactttgcc atagcggggg
     5401 gtatattgcc gccacggtcg agtcgccggc cgcaggagaa agccatgctt gcccgcacga
     5461 tgaagatgac agtggttgca ctggccttag gcgccgcggt gcccaccttt gctgaaacgc
     5521 tggccatcga gggccaggtc gccatcaagc ccgccgggat cgaaaccccg cagcgcggat
     5581 cctccatgac cgccgtcgaa cagaaattcg gcgccccggc caacaaggcg ggtccggtag
     5641 gcaatcccgc catcaccaag tggttctacc cgaacttcgt ggtggtgttc gagaacgaca
     5701 aggtgttgca cgcagtcgtc gtcgccagct gaacgtccgc gccgggcggc ctcgccgccc
     5761 ggtatttctc attacggatt ggcgcgcgcg cattcgaccg cgcgacgctc cgcgagcatc
     5821 gcgggaatgt cttccacccg aaacccacgc ttggcaatct tgcgtccgcc ggcgacgcgc
     5881 agccattcgt cttcggcggg cgtttcctct tcagcgacat cttcgaacgc ggccacgtcg
     5941 gagcccttgt atcgcacggg cgcgagccac ttcgcgtaat catgtggctt ccagccctgg
     6001 ggcgagaact cataccgttc gtttggcgct tcgaagatcg gcaggaccct gttgcccgac
     6061 gcgtcgcgcg ccgcgaattc gatgtacgcc aggttccagg tgtcggactt gcgcgtgagg
     6121 aacagcacgg gctgcctgag ctcgtcatcg aagcgggtat gcagcaccgc ggcgatagtt
     6181 agccggccgg tatcgatgtc gccaaagtag acctcccgca cgcggacgtc gacgtcccag
     6241 gtccagctca tgacgatctg gtcgtcttcg agcgggccga tcggccccgg ccgatagtcg
     6301 agaaatcgcg ccagcacgac ggcatcgatg caatcgggca gcggctcgac cgcctcgggc
     6361 tccccggcgg ccatggcaag cggcgacgcc accaccagaa gcgacaagag tctgcgaaac
     6421 atgccgcgag tatcaaccat aggcagaacg cccgtctgcc ggtaagctgc cgcgctcccg
     6481 aaatgccctc cctgcttcac ccccccattc ccgcgcccca ggcgcgccag cgctggcatc
     6541 agctgtacgg ctccgcgcgc agcctggcgc tggccgaagc cgttgaatcg gacaaccggc
     6601 cctacctcgt catcgcgcgc gacgcgcgcg aactcgacca gcttcgttcg gagctcgcgt
     6661 tctttctcgg gggcagccgc gccattcacg tgttgccgga ctgggaagtc ctgccgtacg
     6721 acctgttctc tccgcacccg gacatcatct ccgaacgcct tgccgcgctc gccgagctgc
     6781 cccgcctgaa gtcggccgtc ctgctggccg ccgcggacac gctgggccag cgcatcgcgc
     6841 cgcgcggcta tgtcgacggc cgcaccttca accttgcgat cggcgacaag ctgcccatcg
     6901 agccgctgcg ggcacggctc gtggagtccg gctacgcgtc cgtgtcgcag gtcagcgcgc
     6961 ccggcgaatt cgcgttgcgc ggatcgctgt tcgacgtcta tcccatgggc gccagtacgc
     7021 cgctgcgtgt cgacctgttc gatgacgaaa tcgaagtcat ccgcgagttc gatcccgaaa
     7081 cccagcgctc cggcgagtcc ctgaagcagg tgcgattgct gccgggccgc gagatgccgc
     7141 tggacgccga ctccgtcaag gcgttccggc tgcgttaccg caagcggttc gagggtgacc
     7201 cgacgaagag cgtgatctat cgcggcgtca gcgacggcct cgcgccgccg ggcatcgagt
     7261 tctacctgcc gctgttcttc gacaccaccg acaccctcct cgactatcta ccgaaggaca
     7321 ccgtggtctg cgcgggcgcg gaagtgccgg cggccgtgga ccgcgcggcg cgcagcatcg
     7381 ccgagcgttt cgaggagcgg cggcacgacg tcgaacggcc ggtgctcgcg cccgatgagc
     7441 tgtacctgcc ccccgaggaa ctgcaggcgc gactcgacac cttcacgcgt gtcgacctcg
     7501 aaagtttcaa gatcgaagac gaaacgaaag gtttcaactt cgccaccgcc gcgccgcagg
     7561 agtggcgcgt cgacctgcgt tcggagcggc cgctcgcgcc gctcgagaat tttctcgatt
     7621 cgtttccggg acgtgtattg ctggccgcgg actcggccgg gcggcgcgaa gtgctgctcg
     7681 agatgctgcg caagcatggg ctcaaacccg ttggcgtcgc cggctggcac gagttcgtcg
     7741 acagcaaagc ggcgttcgcg ctgtgtatcg cgccggagat ggccggtctc aacatcctca
     7801 cccctccgat cgcggtgctc ggcgaggccc agctcttcgg ccaacgcgcg cggcaggagc
     7861 ggcggcggcg gcgcgcccaa tccgatccgc aggcgatcct gcgcgacctc accgcactcg
     7921 gaccgggctc gcccgtcgtg cacgaagaat atggcgtggg ccgctatctc ggcctgcagg
     7981 tcatgcaggt cgccgggcag gacggcgaat tcgtggtgct cgaatacgcg ggcggcgaca
     8041 agttgtacgt gccggtgcag cagctccacc tcgtgtcgcg ttattccggc gccgcgcccg
     8101 aaagcgcgcc gctgcacaaa ctcggcaccg accagtgggc gcgtgcgcgc aaacgcgccg
     8161 ccgacaagat ccgcgacgtg gccgccgaat tgctggacct gtacgcgcag cgccaggcga
     8221 agcgcggccc gtcgttgccg cagcgcgaac tggaatacca ggcgttcgcg agcagcttcc
     8281 cgttcgagga aaccgccgac caggccgagg ccatccgcca ggtgctgcag gacctggcct
     8341 ccgagaagcc catggatcgc gtggtctgcg gcgatgtcgg cttcggcaag accgaagtcg
     8401 cgatgcgcgc ggcttttgtc gccgcgcagg ccggcaagca ggtggcggtg ctcgtgccca
     8461 cgacgctgct cgccgaacag cacaccaaca atttccgcga ccggttcgcc gactggccgg
     8521 tgcgcatcga gtcactgtcg cggttccgca cgggcaagga gtcgaacacg gtgctcgaag
     8581 gcatcgaacg cggaaccgtg gacatcgtca tcgcgaccca gaagctgctg cacgccaatg
     8641 tgcgcttcaa ggatctcggg ctcatcatcg tcgacgagga acatcgcttc ggcgtacgcg
     8701 acaaggagaa gctgaagacg ctgcgcgccg aagtgcacgt gctgacgctc acggccacgc
     8761 cgattccgcg cacgctgaac atggcgctcg gcggcctgcg cgacctgtcg ctgatcacca
     8821 cgccgcccgc cgaacgtctt gccatcaaga ccttcgtcac cgagtggcac gctcccacgc
     8881 tgcgcgaagc cgcgctgcgc gaattccgcc gtggcggcca ggtgtacttc gtgcacaacg
     8941 tcgtcgagac catcgagaag acggcgcagg aaatcgccag gctcataccc gaagccgaag
     9001 tgcgcgtggg ccatgggcag atgcgcgagc gcgacctcga gcagctcatg gtcgatttct
     9061 accatcgccg tttcaatttc ctggtgtgca ccaccatcat cgaaagcggc atcgacgtac
     9121 ccacggccaa caccatcatg atcgaccgcg ccgaccggct gggcctggcg cagttgcatc
     9181 agctgcgtgg ccgcgtcggc cgctcgcatc accgcgcgta tgcctacctg ctgacgccgc
     9241 cgcgcaaggc gctgtcgctc gatgccgtga aacggctgga ggccatcgag tcgctggagg
     9301 acctgggcgc cggcttcgta ctcgccacgc acgacctcga gatccgcggc gccggtgagt
     9361 tgttaggcga gtcgcagagc ggcgaactca ccgagatcgg cctcaccatg tacctcgaca
     9421 tgctcgagca ggccgtgaag gcgctcaagg aagggcgcga gccggcgctc gaccgtccgc
     9481 tctacgccac ggcggaagtg gagttgcggc tgccggcgtt gttgccggag agcttcgtcg
     9541 ccgatgtccc ggtgcgcctg gccctgtaca agcgcctggc cgccgcgccc gacaatgccg
     9601 ccatcgatgg gctgaccgag gagatcgtcg accgcttcgg accgctgccg ccgcctgcca
     9661 cgaatctgtt gcgcgtcgca agactcaaac tagccgcgcg ctccatcgga atccgccgac
     9721 tcgatctcgg gccccagggt ggttacgcgc tgttcgagga acagaaccag gtggaccccc
     9781 gcgcggtcat acggctggtg caacacccgg atcgcgacta tcggctggaa ggccccctca
     9841 agctgcgcat atcgcgcgag aacgaagaag acacggagcg cttcgagttc gccgagtctt
     9901 tcctggcgga gctcaagggc ggccagaagc agccggatcc gaagaagccc gcggcgaagc
     9961 cgcccgctcc gggcaaacga tagccaaccg gtatacttcg cgcccatgac gaccaccgtc
    10021 cgtaccctgt tgctcgcgct gatcctcggc gccgccctgc ccccggcctc ggcgcagacc
    10081 gcgcccaacg cccgggtcta caccatcgag ctcatcgtgt tccgtaacat gagcggccag
    10141 ggtggtcccg aagactggag cgtgaagccc gtggcgcggg gtccggacac gccggatgcg
    10201 ccggtcaccg gcaagttcgt gcaaaccgtg ccggcatcgc agttccagct caacgaggtc
    10261 gcgcggaaac tgcagaacac ggctaactat cagcccatcg cgcacttcgc gtggcagcag
    10321 accgcgagtt cctggggttc gcgcgccggg ttcacggtgg ccaaactcgc cgggtccgcg
    10381 cccggcctgt ccggcatcat ctatctcgag agcggcacgt accttcacct cggcatgtcg
    10441 ctcaactacc agacgtcgaa tccgccctcc ggcctcgccg ccgcgccggg cacggtgttc
    10501 acgctgagcg aggcacggcg catccgcgtc gacaagccga gttactacga ccatcccgcg
    10561 ttcggtgtcg tcgcgctggt cacgcccgca aaccgctcga cgggcggaag atagttagtt
    10621 tccgcttccg tcgatggaag ttgccgcgct gcaagacgcg aaactgagac aaaattccat
    10681 ggtgcaggca tccgcgtcgc gagcagtatc cctgcaaacg cgacaaggag tgcgccatgg
    10741 gaatgtcgag acgtgatttc acgctgttga tgtcgtccgc cacggcggcg ctcgtcaccc
    10801 caccattgct cgcggcgcct ctcaaggtcc ggcgcggaat ccacacgctg agctcggccg
    10861 aactcgacgt tttccgtgcc ggcatcagcg agatgaaggc gctgcccacc gaggatttcc
    10921 gcagctggat gtaccaggcc ggagtccacg gcagctccgt ggccgaacag gccgccattc
    10981 ccgacggtgc cacctactgg cggcaatgca aacacggtag cccgcatttc ctgacctggc
    11041 atcgctggta cctgctgttc tgcgaggaaa tcctccggct cctcgccgat gactgtcact
    11101 tcacgatgcc gtactgggac tacatcgccg acaacttcct gcccgagccg ctgcgtttac
    11161 cggcggacgc gtccaactcg ctgtacgacg gcactcgcaa tggcctggtc aacaacggca
    11221 cgaacggaat ctcgggattg cacaccgatg cgctcgatga actggacttc gacacattct
    11281 cgaatgacct ggcctcgaat ccacacaaca cggtgcacaa ccagatcgtc ggcaacatgg
    11341 gcgcggtcgc caccgcgggc cgcgacccgg ttttctggct gcaccactgc aacgtggatc
    11401 ggtactggga gtgctggatc cgtcagggtg gcggacgcac gcatccgggc agcccgtggt
    11461 ccgatgagga attcccgttt cattcgctga ccggccggcg cgacgtcatc gtggccgacg
    11521 ggctgcgtac cgcgcacctc ggctacacct acgacaacct gccgtgcggc cgccgcgtga
    11581 tctgggaact gccggactgg atcaagacgc tgaaattcga gc
//
LOCUS       DATDEU010000343         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1959161, whole genome shotgun sequence.
ACCESSION   DATDEU010000343 DATDEU010000000
VERSION     DATDEU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959161"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VIV63_08165"
     CDS             complement(<1..1556)
                     /locus_tag="VIV63_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124611.1"
                     /translation="MRPLRDALYAALITFSTSVTGLSIIYLQARDAQMGAVRSELLNL
                     ARTTAAQVDGDLHRTIVSPSQAGSPEHLRALAPLVRMHRAAKDVIYVYTCVQRNGRLN
                     WVLDTSYQYRVPGKDASSTPFPFMMEYRVKDAEFMRAMNEHIAIANTLPVRSEHHSYI
                     SAFAPVYDSQGEFVAILGVDMVLDALDARMASLRRASAVALFVITILSCVAGYVALRI
                     RKFSAAIVAKLRAARARAEENAAAAESASRAKAVFLAMMSHEIRTPMNGMLGVAELLR
                     TMSPDPAQRKLLDILGSSGESLLRIINDILDFSKIEAERLELRPKPFEIRGLLDELDM
                     LLNAQARPKQVTFVIDADPQLPAALNGDRQRLSQILLNLGTNAVKFTDRGEVRLVLRV
                     LAIAEGNARIAFTMRDTGIGMNPDALGRLFSPFTQVAEAGRHRGGTGLGLVITQKLVN
                     LMGGEIQVASEPGKGSSFTFSLELPVAEVAAKAATARVQHLQSLTVLVAEDNAVNQVI
                     IEAMLKQLGH"
     gene            1751..>2805
                     /locus_tag="VIV63_08170"
     CDS             1751..>2805
                     /locus_tag="VIV63_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124612.1"
                     /translation="MLRKTLLTLAGTGCLMAAGWCQAGINEWTHTGPEGGIAKAIAFH
                     PTEPDTVLVSTGAGIFRSTDNGLHWERTLRNTEFDVTDIRFDPTSASRVVAFNQAVGF
                     SENGGQTFSGFYLPNNEPLSSMAVSPNGTVYVVAFSGAMFRTTSFGPTWAQITVPWGG
                     GNPFHRITADPGNANVLYVCRPGTGLYKTIDAGVTWTLLPGSPGSRNEVDVAWQLAVK
                     PGDSNVLVAATPDGFMRSGDGGATWATQMPGTYPASVAFDPATPANVIAISFAGDILR
                     STDSGATWPVNLQRPRLRANNSTQIVVHPSVVGRLFVGSSDGPLFSADGGATLETRAN
                     GIYAARIRNFSAAFNGD"
BASE COUNT          503 a          893 c          916 g          493 t
ORIGIN      
        1 ctgtggccca gctgcttcag catggcctcg atgatgacct ggttcaccgc attgtcttcg
       61 gccacgagca ccgtcaggct ttgaaggtgt tgcacgcggg cggtcgcggc cttggcggcg
      121 acctccgcca cgggtagttc gagactgaac gtgaacgacg aacctttccc gggttcgctg
      181 gccacctgga tttcgccgcc catgaggttc acgagcttct gcgtgatgac caggcccagg
      241 cccgtgccgc cgcgatgccg gccggcctcg gcaacctgcg tgaacggcga gaagaggcgg
      301 ccgagcgcat ccggattcat gccgatgccg gtgtcccgca tggtgaaggc gatgcgggca
      361 ttgccttcgg cgatcgcgag cacacgcagg accagccgta cctcgccgcg atccgtgaac
      421 ttgacggcat tggtgccgag attgagcagg atctgcgaga gccgctgacg atcgccattg
      481 agcgccgcgg ggagctgcgg atcggcgtcg atgacgaacg tcacctgctt gggtcgcgcc
      541 tgtgcgttga gcagcatgtc gagctcgtcg agcagtccgc ggatctcgaa cggtttcggc
      601 cgcagctcga gccgctccgc ttcgatcttc gagaagtcga ggatgtcgtt gatgatccgc
      661 agcagcgact cgccgctgct gccgagaatg tcgagcagct ttcgctgcgc ggggtccggt
      721 gacatcgttc tcagtagttc ggccacgccc aacatgccgt tcatcggcgt gcggatttcg
      781 tggctcatca tcgcgaggaa cacggccttg gcgcggctcg cggattcggc cgccgcggca
      841 ttttcctcgg cgcgcgcccg cgcggcacgc aacttcgcga cgatggcggc actgaatttg
      901 cggatgcgca gcgccacgta gccggcgaca cacgagagga tcgtgatgac gaacagcgcc
      961 acggccgatg cacggcgcag cgatgccatt cgtgcatcga gtgcgtcgag caccatgtcc
     1021 acgcccaaga ttgcgacgaa ctcgccctgg ctgtcataca ccggcgcgaa cgccgatata
     1081 taggagtgat gctcggaacg caccggcaat gtgtttgcaa tggcgatgtg ttcgttcatc
     1141 gcgcgcatga actcggcgtc cttgacgcgg tactccatca tgaaagggaa cggtgtcgac
     1201 gatgcatcct tgcccgggac gcggtattgg tacgacgtat cgagcaccca gttgagccgg
     1261 ccgttccgct gcacgcaggt gtacacgtag atcacgtcct tcgcggcgcg atgcatgcgc
     1321 accagcggcg cgagcgcgcg cagatgttcc ggacttcccg cctgcgacgg cgacacgatc
     1381 gtgcgatgaa gatcgccgtc gacctgcgcg gcagtggttc gcgccaggtt cagaagctcg
     1441 ctgcgtacgg cgcccatctg cgcgtcgcgt gcctgcagat agatgatgga aagccccgtg
     1501 accgacgtgg aaaaagtgat gagcgccgcg tacagcgcgt cgcgcagcgg gcgcactttt
     1561 ctactggtgg tgggagtagg ggcagacacc caccgatcat gcccgtacgg gcttgcggcg
     1621 tcggtgagcg ccgtcgcagg cggggcgcgg cgaaaaactc gaaaaaccct agggaaaaag
     1681 cgttctggag tgtcaccgat cggccgttcg tgacaccgta ctcaatgaca tatccatttt
     1741 gggtttcacg atgttaagga aaacgctgct gacgctggcc ggtacgggtt gtttgatggc
     1801 cgcgggctgg tgccaggccg gcatcaacga atggacgcac acgggtccgg aaggcggcat
     1861 cgccaaggcg atcgccttcc atccgacaga gccggacacc gtgctcgtca gcacgggcgc
     1921 cggcatcttc cgcagcaccg acaacggact gcattgggag cgcacgctcc gcaacaccga
     1981 attcgacgtc accgacatcc ggttcgaccc gacctcggcg agccgcgtgg tggcattcaa
     2041 tcaggccgtg ggattcagcg aaaacggcgg gcagaccttc tccggctttt atctcccgaa
     2101 caacgagccg ctgagctcga tggcggtgtc gccgaacgga acggtatacg tggtcgcctt
     2161 cagcggcgcg atgttccgca ccaccagctt cggaccgacc tgggcccaga tcaccgtgcc
     2221 ctggggaggc ggcaatccgt tccaccggat caccgccgat cccggcaacg cgaacgtgct
     2281 gtacgtctgc aggcccggca ccgggctata caagaccatc gatgccggcg tcacgtggac
     2341 gctgctgccg ggcagtcccg gcagtcgcaa tgaggtcgat gtggcctggc aactcgcggt
     2401 caaacccggc gacagcaacg tgctcgtcgc ggcgacgccc gacggattca tgcgctcggg
     2461 tgatggtggt gccacctggg caacgcagat gcccggcact tatccggcat cggtggcgtt
     2521 cgatccggcc acgcctgcca acgtgatcgc gatttcgttt gccggcgaca tcctgcgcag
     2581 caccgacagc ggcgcaacgt ggcccgtgaa tctgcaaagg ccgcggctgc gagctaacaa
     2641 ctcgacgcag atcgtcgtgc atccatccgt ggtcgggcgc ctcttcgtgg gctcgtccga
     2701 cgggccgctg ttcagcgccg acggcggcgc cacgctcgag acgcgagcca acgggatcta
     2761 cgcggcacgc atcagaaact tttccgcagc gttcaatggc gacat
//
LOCUS       DATDEU010000344         5527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_208292, whole genome shotgun sequence.
ACCESSION   DATDEU010000344 DATDEU010000000
VERSION     DATDEU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208292"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /gene="clpX"
                     /locus_tag="VIV63_08175"
     CDS             complement(<1..861)
                     /gene="clpX"
                     /locus_tag="VIV63_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY6124613.1"
                     /translation="MTEDTRGRHDDGKLLYCSFCGKSQHEVRKLIAGPSVFVCDECVE
                     LCNDIIREELEDRAERARDKLPKPSEIKKVLDEYVIGQERAKKTLSVAVYNHYKRLQT
                     RGGTAGRGKDDVEISKSNILLIGPTGCGKTLLAETLARLLNVPFTIADATTLTEAGYV
                     GEDVENIIQKLLQKCDYDVEKAQTGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALL
                     KLIEGTMASVPPQGGRKHPQQEFLQVDTSNILFIVGGAFAGLEKIVLNRTQKSGIGFT
                     SDVRSVSTRPH"
     gene            complement(1045..1662)
                     /gene="clpP"
                     /locus_tag="VIV63_08180"
     CDS             complement(1045..1662)
                     /gene="clpP"
                     /locus_tag="VIV63_08180"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEY6124614.1"
                     /translation="MNERALNLVPIVVEQTARGERSYDIYSRLLKERVVFAVGPVEDY
                     MANVIVAQLLFLESENPEKDIALYINSPGGSVSAGLAIYDTMQFIKSDVSTICIGMAA
                     SMGAILLAGGAKGKRFMLPNSRAMIHQPSGGYQGQASDIHIQAQEILKIRGRLDEILA
                     HHTGQTVEKIKLDSERDNFMSPTEAVAYGLVDKLLDKRVAAPTPA"
     gene            complement(1681..2985)
                     /gene="tig"
                     /locus_tag="VIV63_08185"
     CDS             complement(1681..2985)
                     /gene="tig"
                     /locus_tag="VIV63_08185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY6124615.1"
                     /translation="MQVSLTATGGLERRLEVAIPAAQIDGEVSQRLTKISRTARLKGF
                     RPGKAPLAVIRQQYGEQVHGEVVNDLMRASFSEAVAREKLNPAGGPRIEPIAMNPGAD
                     LKYAAVFEVLPEVKLKPLADIAIERPVASVADKDLDAMIDTLRKQRPAFNEVSRGVVA
                     TDRVTVDFTGKIDGVEFDGGSGEGVPIVIGANQVMKEFEDALLGASASDTREFNATFG
                     ADHTNKKLAGQTATFNVKIVKVEEQVPAPLDEAFAKGFGIADGNIETLRAEVRANMER
                     ELAEAIRQKVRTQILEGLYTQNPLELPRQMVEEQIQELQVEMLRRAGVKDAKQLPPRE
                     PFEQPARRRVALGLLMSELVRNANLKVSREAVQEKLNELAASYSNPDEVRRAYLQNAD
                     MMRQIESQVLETQAIEWVLGQAKVADKATTFAELTQFGQGDA"
     gene            complement(3023..3107)
                     /locus_tag="VIV63_08190"
     tRNA            complement(3023..3107)
                     /locus_tag="VIV63_08190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3071..3073),aa:Leu,seq:tag)
     gene            3255..3626
                     /locus_tag="VIV63_08195"
     CDS             3255..3626
                     /locus_tag="VIV63_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124616.1"
                     /translation="MGQLGNLVVVGLLTIASGLLDARGFVYASRAWPAGQLDLKVALA
                     SLAAFCGGITAYIIAIKFMQNAGILSVTLQTGIWFVITAVGISVMDGTIVQWTRSQQV
                     VGIGVAVALGWLITTTRAAEG"
     gene            3648..3845
                     /locus_tag="VIV63_08200"
     CDS             3648..3845
                     /locus_tag="VIV63_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY6124617.1"
                     /translation="MDSRLIEMLACPVSKAPVFFSEGRQLIVCPTSKLAYPIKDGIPA
                     MLPEDAIKLEDNDPLLTQKTS"
     gene            complement(3862..5226)
                     /locus_tag="VIV63_08205"
     CDS             complement(3862..5226)
                     /locus_tag="VIV63_08205"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6124618.1"
                     /translation="MAKQPSRDDPSKPKAHGYAYKKQFYQSADVAADYDEHRFRTPKR
                     QRRNARKWDAIQKALALTENVKTIVDLPCGTGRFTGNLARAGYQVVGSDISIEMMQQA
                     AKLPAVQHANIAGYVRADAEALPFRTKSADCLMSIRFLFHVDGETRRRMLREFGRVSR
                     RWVIADYRHKYSARYGVWKIASTLGLTKRPFERVSVKSMKAEFEDAGLRVLKIVPVRR
                     WLSDKWVVLAEAGHVDPASLAEKLKGTEYADLQVTEKLGEGKRSVVYRARWRGREIGL
                     KVYKPAAIANHARKHKLPLAEFENRRNRAFFEARGMAKYVAEPLGFVVEPGFQMSLQE
                     CLDGEVYYFAQRDHADFISPRFMDDLAELVSLSHQAKLYDIDLHAMNVMVDRKAGTPK
                     LFDFNQIPFTERPQNPFVGLALKLGLLGRGSRDLRKLARFKNFDRVERKLLKFYKESS
                     TSKA"
     gene            complement(5271..5346)
                     /locus_tag="VIV63_08210"
     tRNA            complement(5271..5346)
                     /locus_tag="VIV63_08210"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5311..5313),aa:Lys,seq:ctt)
BASE COUNT          976 a         1714 c         1712 g         1125 t
ORIGIN      
        1 gtgcggccgc gtggacaccg agcgcacgtc ggaagtgaag ccgatgccgc tcttctgcgt
       61 gcggttcagg acgatcttct cgagaccggc gaacgcaccg ccgacgatga acaggatgtt
      121 cgaggtgtcg acctgcagga actcctgctg cggatgcttg cggccaccct gcggcggcac
      181 cgaggccatc gtgccctcga tcagcttcag cagcgcctgc tgcacgcctt cacccgacac
      241 gtcacgcgtg atggacgggt tgtcggactt gcgggaaatc ttgtcgattt cgtcgatata
      301 gacgataccg gtctgcgcct tctcgacgtc gtagtcgcac ttctgaagga gcttctgaat
      361 gatgttctcg acgtcttcgc ccacgtaacc ggcttcggtc agcgtggtgg cgtccgcaat
      421 cgtgaacgga acgttgagca gacgcgcgag cgtctcggcg agcagggtct tgccgcagcc
      481 ggtggggccg atcagcagga tgttgctctt ggaaatttcg acatcgtcct tgccacggcc
      541 ggcggtgccg ccgcgggtct gcagacgctt gtagtggttg tacaccgcga ccgagagcgt
      601 cttcttcgcg cgctcctggc cgatgacgta ttcgtcgaga actttcttga tctcggaagg
      661 tttcggaagt ttgtcgcgcg cgcgttcggc gcgatcttcg agttcttcac gaatgatgtc
      721 gttgcagagc tcgacgcatt cgtcgcagac aaaaaccgac ggaccggcga tcagcttccg
      781 aacttcgtgc tgactcttgc cgcagaagga gcagtacaac aacttgccat cgtcgtgccg
      841 tccgcgagtg tcctcggtca tcgtcgctcc gtgtggcgct tacaggcatg tgcctgcgag
      901 ctttgggatt ctatatatca cggctgttcc gcagaaccca aggggcactc tggcggaagc
      961 cgttgtttat acgggatttg tcgagaactg cgaggcggct cgcaaatgac ggccgcagcc
     1021 ggccgccgtc tggaaactct tggactaggc cggtgtcgga gccgccacgc gcttgtctag
     1081 taacttatct acaagcccgt aggcgaccgc ctcagtgggg ctcatgaaat tgtcgcgttc
     1141 ggaatcgagt ttgatcttct cgaccgtctg gccggtgtgg tgcgccagga tctcgtccag
     1201 acgcccgcga atcttgagga tttcctgggc ctggatgtgg atgtcgctgg cctggccctg
     1261 ataaccgccc gacggctggt gaatcatggc gcgcgagttc ggcagcatga atcgcttgcc
     1321 cttggcgccg ccagccagaa ggatcgcgcc catggacgcc gccatgccga tgcagatggt
     1381 gctgacgtcg gacttgatga actgcatggt gtcgtagatg gccaggcccg cgctcaccga
     1441 tccgccgggc gaattgatgt acagagcgat gtccttttcg gggttctccg actcgaggaa
     1501 caggagctgg gcaacgatga cgttggccat gtaatcctcg accggaccca cggcaaacac
     1561 cacgcgctct ttgaggagcc gcgagtagat gtcgtaagag cgctcgccac gagcggtctg
     1621 ctccacgacg atcggcacca gattcaaagc gcgttcattc atcgtctact cctaattact
     1681 tcaggcgtcg ccctgaccaa actgtgtgag ctcggcgaaa gtcgtggcct tgtccgccac
     1741 cttcgcctgg cccaacaccc attcgatggc ctgggtttcg agtacctgcg actcgatttg
     1801 acgcatcatg tctgcattct gcaagtaagc gcgtcgcact tcatcagggt ttgaataaga
     1861 cgccgccagt tcgttcaact tttcctgcac ggcttcgcgg ctcaccttga gattcgcgtt
     1921 gcgcacgagt tcgctcatca gcaggccaag cgcgacgcgg cgccgggcgg gttgttcgaa
     1981 cggttcgcgc ggcggcagtt gtttggcgtc cttcacaccc gcgcgacgca gcatttcgac
     2041 ctgcaattcc tggatctgct cttcgaccat ctgccggggc agttcgagcg ggttctgcgt
     2101 gtacagccct tccaggatct gggtccgcac cttctggcgg atcgcctcgg cgagctcgcg
     2161 ctccatgttg gcgcgcactt cggcgcgcag cgtttcgatg ttgccgtcgg cgatgccgaa
     2221 acccttggca aaggcctcgt cgagcggcgc gggcacctgc tcttccacct tcacgatctt
     2281 cacgttgaac gtggcggtct ggccggccag tttcttgttg gtgtggtcgg cgccgaaagt
     2341 ggcgttgaac tcgcgcgtgt cggaagccga ggcgccgagc agcgcgtctt cgaattcctt
     2401 catcacctgg ttggcgccaa tgacgatcgg cacgccctca ccactgccgc catcgaattc
     2461 cacgccgtcg atcttgcccg tgaagtcgac ggtgacgcga tcggtggcga cgacgccgcg
     2521 cgaaacttcg ttgaacgccg gacgctgctt gcgcaacgtg tcgatcatgg cgtcgagatc
     2581 cttgtcggcg acgctggcca ccgggcgctc gatggcgatg tcggcgagcg gcttcaactt
     2641 cacttcgggc aacacttcga agaccgccgc gtacttgaga tccgcgccgg gattcatcgc
     2701 gatgggttcg atacgcgggc caccggccgg attcagcttc tcgcgcgcga cggcctcgga
     2761 gaacgaagcg cgcatgagat cgttgacgac ctcgccgtgc acctgctcgc cgtactgctg
     2821 acggatgacc gccagcggcg ccttgccggg gcggaaaccc ttgagccgcg cggtgcgcga
     2881 gatcttggtc aggcgttgcg acacttcacc gtcgatctgc gccgcgggaa tcgcgacttc
     2941 gagacggcgt tcgagtccgc ccgtggcggt cagagaaacc tgcatatcaa ttcctttcgt
     3001 caaacttgtt cacgcaggcg agtggtgcga aaggagagac tcgaactctc acgggttacc
     3061 ccactggaac ctaaatccag cgcgtctacc agttccgcca ctttcgcccg gcccgtattg
     3121 cctaaaaaag gcggccacgc gcgaggggcg cggattatag cgaaacccgc ccgctcaacc
     3181 tacgcgttgc acgccgaggg cgggttatcg gggatttggc tatgcttccc gcgcctcagg
     3241 aggagggaca taagatgggt caattgggaa atctcgtcgt ggtggggctt ttgaccatcg
     3301 catccggcct gctcgacgcc cgcggcttcg tatatgcctc gcgcgcctgg ccggcgggcc
     3361 agctcgacct caaggtcgcc ctggcatccc tggccgcatt ctgcggcggg attacggcct
     3421 acatcatcgc catcaagttc atgcagaacg ccgggatcct gagcgtgaca ctgcagaccg
     3481 gcatctggtt cgtgatcacg gccgtcggca tttcggtgat ggacggcacc atcgtgcagt
     3541 ggacccgcag ccagcaggtg gtcggcatcg gggtcgcggt ggccctgggg tggctcatca
     3601 ccaccacgcg agcggcggaa ggctgaacaa agctctatcc ttcgcccatg gactcaagac
     3661 tcatcgaaat gctcgcgtgc ccggtgtcga aagcgccggt gttcttctcg gaaggccgcc
     3721 agctgatcgt ctgcccgacc agcaagctcg cctatccgat caaggacggc atcccggcga
     3781 tgctgcccga agacgccatc aagctcgaag acaacgatcc gctgctcacg cagaagacga
     3841 gctgaaccgc acggcggccg ctcaggcctt cgaggtgctc gattccttgt agaacttgag
     3901 cagcttgcgt tcgacgcggt cgaagttctt gaagcgcgcg agcttgcgca ggtcgcggga
     3961 tccgcgtcct aacaatccca gcttgagcgc gagaccgacg aacgggttct gcggacgctc
     4021 ggtgaacggg atctggttga agtcgaacag cttgggcgtg ccggctttgc ggtcgaccat
     4081 cacgttcatg gcatgcaggt cgatgtcgta gagcttcgcc tggtgactca gcgacaccag
     4141 ctccgccagg tcgtccatga aacgcggcga aatgaaatcc gcgtgatcgc gctgcgcgaa
     4201 gtaatagacc tcgccgtcca ggcattcctg cagcgacatc tgaaacccgg gctcgaccac
     4261 gaaacccagc ggctcggcca cgtacttcgc catgccccgc gcctcgaaga aggcccggtt
     4321 gcggcgattt tcgaactcgg ccaacggaag tttgtgtttg cgcgcgtggt tggcgatcgc
     4381 cgcgggcttg tagaccttga gtccgatctc gcggccgcgc cagcgcgcgc gataaaccac
     4441 cgaacgtttg ccctccccca gcttttccgt cacctgaagg tccgcgtatt cggtgccctt
     4501 gagtttctcg gcgaggctcg cgggatcgac gtgcccggct tccgccagca ccacccactt
     4561 gtccgacagc cagcggcgca cgggcacgat cttgaggacg cgcaagccgg cgtcttcgaa
     4621 ctccgccttc atgctcttga ccgacacgcg ctcgaacggc cgtttcgtga gacccagcgt
     4681 gctcgcgatc ttccacacgc cataccgggc cgaatacttg tggcgatagt cggcaatcac
     4741 ccagcggcgc gacacacggc cgaactcgcg caacattcgc cggcgcgtct caccgtcgac
     4801 atgaaacagg aaccggatgc tcatgaggca gtccgccgac ttcgtgcgga acggcaacgc
     4861 ctcggcatcg gcgcgcacat accccgcgat gttggcgtgc tgcacggccg gcaacttcgc
     4921 cgcctgctgc atcatctcga tggagatgtc gcttcccacc acctggtagc cggcgcgcgc
     4981 gagattgccg gtgaatcgcc cggtgccaca gggcagatcg acgatggtct tcacgttttc
     5041 ggtgagcgcg agcgccttct ggatggcgtc ccacttgcgc gcgttgcgac gttggcgctt
     5101 gggcgtgcga aaccggtgtt cgtcgtagtc ggccgcgacg tcggcgcttt gatagaactg
     5161 cttcttgtac gcgtagccgt gcgctttcgg cttggaagga tcgtcgcggg aaggttgctt
     5221 tgccattggc ggaatttacc tgccgcctca ggagtctgcg cgcgaaatga tggtggggcg
     5281 tgtaggggtc gaacctacga cctactgatt aagagtcagc tgctctacca actgagctaa
     5341 cgccccatat ttcgggggcg cgactgtacc agagtgaaaa gcggaatcct aagaagtttc
     5401 aaacgcggtc tcgcgagcgg gatgagttcg cgtaaatccg cgcggcgaag gtagggtgcg
     5461 ttccggcacg tatatatatg taagagctca gtcggcggtt ctgcgaaacg ctggcgcaaa
     5521 tcgatca
//
LOCUS       DATDEU010000345         3398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1522438, whole genome shotgun sequence.
ACCESSION   DATDEU010000345 DATDEU010000000
VERSION     DATDEU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522438"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VIV63_08215"
     CDS             <1..535
                     /locus_tag="VIV63_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6124619.1"
                     /translation="NALRWLVSEADEREKLAMCSINLSGQSFGDDKFLPFVIEQFQKS
                     GIDAAKICFEITETAAVASFSQANRFIQALKELGCRFALDDFGTGLSSFGYLKHFPVD
                     FLKIDGSFVREILHDPIDREMVRSINEIGHLTGKKTIAEFAENAEIIQMLTSLGVDYA
                     QGYGIAQPQRVLKAVNA"
     gene            622..2985
                     /locus_tag="VIV63_08220"
     CDS             622..2985
                     /locus_tag="VIV63_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEY6124620.1"
                     /translation="MRLPRIVGLVFLFAAPLAHSQALTLWYDKPATDWEKEALPIGNG
                     RMGVMVFGGVESERLQISEKSLWTGGPGTEGGYDYGLPADSQAALMRSIGKQLTDGTQ
                     LAPEAVARQLGRKMHNYGDYQSFGDLIIERENDGSPVTGYRRDLDLDTATARVSFKQG
                     DAGYRREYLVSYPDQALIARWYGTSVQKLRVRFAVPDNRTVNVRAEGTRIVISGALKS
                     NGLQYAAELRVIPECGTVNVDGDSLRIESECQITFVLAARTNYRMQYPDYREPSADPV
                     AAVAKEAAGAARERFAAVSSRHTQDYQALFRRVSLRLDSGATKLPTDRLRAQYGSGNA
                     AADRQLEQLYFQYGRYLLIASSRTGSLPANLQGVWNDKATPPWNADYHVNINLQMNYW
                     PAEVANLSETAPPLFDFVDHLVTPGKLAAQRFFGASGWTMFLNTNAWGYVGPIDWPTA
                     FWQPEASAWLAQHFYEHYLFSQDAEFLKKRAWPVMKGAAEFWLDALVTDPRDGKLIVS
                     PSYSPEHGPFTAGAAMSQQIVADLFVNTSEAAQLVGDRAFAKRVDDALAKLDRGLRIG
                     KWGQLQEWKADLDDAKDDHRHVSQLFALHPGRAIDPVKNPELVAAARATLDARGDAST
                     GWSRAWKINFWARLRDGDRAHKLLAGLLRDSTLPNLWDTHPPFQIDGNFGATAGIVEM
                     LLQSHNGELHVLPALPKAWSTGSVKGIRARGDVTADIDWDSCGLVKLVLTTGHAGPVS
                     VRSSSFEKGFSGSVKTEGAADWRRFMARRGGEYTFMRTAAAACAGVP"
     gene            3031..>3398
                     /locus_tag="VIV63_08225"
     CDS             3031..>3398
                     /locus_tag="VIV63_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6124621.1"
                     /translation="MNPYIVPWMDDDLEVFRDTVVRFIETEMVPHDARWREQHHVDRE
                     TWRKAGDTGLLLLDVPAGYGGGGGDFRHEAILYTELARRGIAAFGQGVHSMCAHYVLN
                     HGSEDQKQRFLPRMASGELIG"
BASE COUNT          629 a         1126 c         1072 g          571 t
ORIGIN      
        1 gaacgcgctg cgctggctgg tcagcgaagc cgacgagcgc gagaagctcg ccatgtgctc
       61 catcaatctt tccggccaga gtttcggcga cgacaagttc ctgccgttcg tcatcgagca
      121 gttccagaag agcggcatcg acgccgcgaa gatctgcttc gagatcacgg agaccgcggc
      181 cgtggccagc ttctcccagg ccaaccgatt catccaggcg ctgaaggaac tcggctgccg
      241 cttcgcgctc gacgacttcg gcaccggcct gtcgtcgttc ggttacctca agcacttccc
      301 ggtggacttc ctcaagatcg acggcagctt cgtgcgcgaa atcctgcacg accccatcga
      361 ccgcgagatg gtgcgttcga tcaacgaaat cggccacttg accggcaaga agaccatcgc
      421 cgagttcgcc gagaacgccg agatcatcca gatgctcacg tcgctgggcg tcgactacgc
      481 gcagggttat ggcatcgccc agccgcagcg cgtcctgaag gccgtcaacg cttgaggaag
      541 gcctgacggc cacttcggaa aacgccgccg gagcggcggc gtccatgatt gttatgatcc
      601 cggtgtcatc agggggatca catgcgactg ccgcgaatcg ttggccttgt cttcttgttt
      661 gccgccccgc tcgcgcattc gcaggcgctg accctctggt acgacaaacc cgccacggac
      721 tgggaaaaag aagcgctgcc catcggcaac ggacgcatgg gcgtcatggt gttcggcggc
      781 gtcgagtcag agcgactgca gatctccgag aagtccttgt ggaccggcgg tcccggcacc
      841 gaaggcggtt atgactacgg cctgccggcc gactcccagg ccgcgctcat gcgctccatc
      901 ggcaaacaac tgaccgatgg cacgcaactg gcgccggaag ccgtggccag acaactcggc
      961 cgcaagatgc acaactacgg cgactaccag agctttggtg acctcatcat cgaacgcgag
     1021 aacgacggct cgcccgtgac gggttatcgc cgcgatctcg acctggacac ggccaccgcg
     1081 cgcgtcagct tcaagcaggg cgatgccggc taccgacgcg agtatctcgt ctcgtatccc
     1141 gaccaggcgc tgatcgcgcg ctggtatggc accagcgtgc agaagctgcg cgttcgtttc
     1201 gcggtgcccg acaatcgcac ggtcaacgtc cgtgccgagg gcacgcgcat cgtgatatcg
     1261 ggcgcgctga aatcgaacgg gctccaatac gccgcggagc ttcgcgtgat cccggagtgc
     1321 ggcaccgtca acgtcgatgg cgacagcctg cgcatcgaaa gcgaatgcca gatcacgttc
     1381 gtgctggccg cgcgcaccaa ctaccgcatg caataccccg actatcgcga gccgtcggcc
     1441 gatccggtcg ccgcggtggc gaaagaggcg gccggcgccg cccgggaacg gttcgctgcc
     1501 gtctccagcc gtcacacgca ggactaccag gcgctgttcc gccgcgtgtc actgcggctg
     1561 gactccggcg cgaccaaact acccacggac aggctgcgcg cccagtacgg cagtggcaat
     1621 gccgccgcgg accgccagct cgagcaactg tacttccagt acggccgcta cctgctgatc
     1681 gcttcgtcac gcaccggctc gctgcctgcg aacctgcagg gcgtgtggaa cgacaaggcg
     1741 actccgccgt ggaatgccga ctaccacgtc aacatcaatc tgcagatgaa ctactggccg
     1801 gccgaagtgg cgaacctttc cgagaccgcg ccgccgctgt tcgatttcgt cgatcacctc
     1861 gtgactcccg gcaaactcgc ggcgcagcgc tttttcggcg ccagcggctg gacgatgttc
     1921 ctcaacacca acgcctgggg ctatgtggga cccatcgact ggcccacggc gttctggcag
     1981 ccggaagcct cggcgtggct cgctcagcac ttctacgagc actatctatt cagccaggat
     2041 gccgagttcc tgaagaaacg tgcctggccc gtcatgaagg gcgccgccga attctggctg
     2101 gatgcgctgg tcacggatcc tcgcgatggc aaactcatcg tgagcccgag ctactcgccc
     2161 gaacacggtc ctttcaccgc gggcgcggcg atgtcacagc agatcgtcgc ggatctgttc
     2221 gtcaatactt ccgaagccgc gcaactggtg ggtgatcggg cgttcgcaaa gcgcgtcgac
     2281 gacgcgctcg cgaagctcga ccgcggcctg cgcatcggga agtggggcca gctgcaggag
     2341 tggaaggccg acctcgacga cgccaaggac gatcaccgtc acgtgtcgca actgttcgcg
     2401 ctgcatcccg ggcgtgcgat cgatccggtg aagaatcccg agctggtcgc cgctgctcgc
     2461 gcgacactcg atgcgcgcgg cgatgccagt acgggctgga gccgcgcgtg gaagatcaac
     2521 ttctgggcgc gcttgcggga cggcgaccgc gcgcacaaat tgctggcagg cctgcttcgc
     2581 gacagcacgc tgcccaacct gtgggacaca catccgcctt ttcagattga cggcaacttc
     2641 ggcgccaccg cgggcatcgt cgagatgctg ctgcagagtc acaacggcga actgcacgtc
     2701 cttccggcgc ttcccaaggc gtggagtacc ggcagcgtca aaggtattcg cgcacgaggc
     2761 gacgtcaccg ccgatatcga ctgggattcg tgcgggctgg tgaaactggt attgaccacg
     2821 ggccacgccg gccctgtaag cgttcgatca tcgtccttcg aaaagggttt cagcggcagc
     2881 gtgaagaccg aaggcgccgc ggattggaga cgtttcatgg ccaggcgggg cggtgaatac
     2941 accttcatgc gcacggccgc cgcggcgtgt gccggggtgc cctgacgtct tcggtaggcg
     3001 tacgcggggc cgcgcgctag gcttggcgcc atgaacccct acatcgttcc ctggatggac
     3061 gacgacctcg aagtgtttcg cgacacggtc gtgcgtttca tcgaaaccga aatggtcccg
     3121 cacgacgcgc gctggcgcga gcaacatcat gtcgaccggg aaacctggcg caaggccggc
     3181 gacaccggac tcctgttgtt agacgtcccg gccgggtacg gcggtggcgg cggcgacttc
     3241 cgccacgaag caatcctgta caccgaactc gcgcgccgcg gcatcgccgc cttcggccag
     3301 ggcgtgcaca gcatgtgcgc gcactacgtg ctgaatcacg gcagcgaaga tcagaagcag
     3361 cgcttcctgc cgcgcatggc gtcgggtgag ctcatcgg
//
LOCUS       DATDEU010000346         7146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1667956, whole genome shotgun sequence.
ACCESSION   DATDEU010000346 DATDEU010000000
VERSION     DATDEU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667956"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..450)
                     /locus_tag="VIV63_08230"
     CDS             complement(46..450)
                     /locus_tag="VIV63_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124622.1"
                     /translation="MANRLPHIAVVDDEESVRLALARMLRASSFDVSIYRSGEEFLES
                     LKSAIPDCVVLDFQMPGLTGRDIQRALSVAKVALPIIIVTAHDQPSLREKVLADGAVA
                     YFTKPLRRDTLIAALDQAIRRAPLSTPSQTGE"
     gene            complement(499..2616)
                     /locus_tag="VIV63_08235"
     CDS             complement(499..2616)
                     /locus_tag="VIV63_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEY6124623.1"
                     /translation="MSAQTIQESSETGKAISRHPLGHAREGLLGTPAAAHVLGATALS
                     AGYYATAQLGEALAFPNAPVSALWLPNALVMGALLIVPRRFWWFYLCAVLPAHLAAQI
                     PLPDVNLARVTIQYVLNCATAFIGAFALTYFEPGDQRFDRVLQSWRLVFFGGLVAPFT
                     TSIVMAALFLTTDVDKSFWLTATVRALTNSFAILTVVPLMVHLSDRLHQQRQHIDLAR
                     VIEASLLALGLVAASLTALFAPQTGAVASAAMLCLPMPMLLWAAARFGVSGSAAATLL
                     FGIVAISGALAGEGPFMSQPDAHGTLSVVLFLVVTGVPLLLLGAALDERRALENARAA
                     SDALHGAVLASIQDKIVILDSSGRIVDANEAWRQLAERIPGTPFVNLKLGEHLLHHCL
                     EVGAKGDAVATRLGTCVTEVIAGRSRLHQLEYAAHAPGGITWFEVTIEPLRRPEGGAV
                     LVWTDVTFRKQAEGKERAQQQQLVHLGRAAVLGELSGAFAHELTQPLTSILGNAEAAL
                     ELLKKPNADLREIGSMVRDIVADDVRAAEVIQRLRAMLTRGEISREPVDLANVIKDVL
                     FLARIDLLTRHVSVDVVCDPYLPLVLGDRIQLQQVVLNLVVNACEAMSNIDVHQRRIT
                     ITARFEGSSCEVVCAIHDRGAGIASEDLERIFQPFVTTKTQGLGMGLAICRSIIEAHG
                     GRLWAENAADGGATFNFTARMGS"
     gene            2783..3379
                     /locus_tag="VIV63_08240"
     CDS             2783..3379
                     /locus_tag="VIV63_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HEY6124624.1"
                     /translation="MLRWSEATFWIAGATLCSLFALAQADSAIGSANALEDFSATLVP
                     PDTSLWAAGRVRDYQASLKEPLQAVAVLTIPSLKLEVPVFDRDDDLALNRGAGLVPGM
                     GLPDQGGNIGIAGHRDGYFRALKDIKVGDRIEIRTHIKVHRFRVTSIDIVDKADNRLL
                     ADTELPVVTLVTCYPFYFLGSAPQRYIVAGAYEWTSET"
     gene            3396..3968
                     /locus_tag="VIV63_08245"
     CDS             3396..3968
                     /locus_tag="VIV63_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124625.1"
                     /translation="MRRSTYLSFIGVTALLGGASVPALAADCASAQFSDEVLARFPNI
                     RAVCSEVIEKDGQQYAVVKGDLVRTGNNAVYMKFKMADGTKSDTRKIETRPEFRVKVD
                     GKPVSVRNLAVGQELTAYVKVSDPVIALEPYESVALETTPLESAPATMASNETGDMPR
                     TASIVPGLGTLGALLLAFAGMLTFFRLRRE"
     gene            4209..4865
                     /locus_tag="VIV63_08250"
     CDS             4209..4865
                     /locus_tag="VIV63_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6124626.1"
                     /translation="MKFTSMMVWAAGLSVLSAGVCAQSRDPGWEFGGDVVYQSSQDVD
                     FDNGSTSFDDDIGVSLFAGYRMSPRLEFQFGLDWSNVGYTGDFVPEDFPGISVSVDAD
                     IEAFTPFAKVNFNFLEGPITPFVTGNVGWSFIDTNIPDGPPQTGCWWDPWYGQVCSTF
                     QETRSTDSFTYGLGLGLRWDLSTGYSLRFVYEKQWYDIDQADSAPDFDRFKIGIVFVY
                     "
     gene            4890..5996
                     /locus_tag="VIV63_08255"
     CDS             4890..5996
                     /locus_tag="VIV63_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY6124627.1"
                     /translation="MRSLCHSCLGLLSLSASPGFANDFPVNTDTELVHDEPSGQTKKK
                     VNFVLAPIPIVNPTIGNGLALAALLLYKFDPKSPASMTAVAAGYTDTDSWGVGILQDS
                     KFSEDRWRVKAGAALAVARYDLYVEEIAPDFHFSTEQRLGGVMAQALRRVTARLYAGL
                     RYQRATVEFPAPDAVQDLIPEDGLDLDIGGLGLVAEWDTRDHSFQPGSGSYLTFRSNF
                     ARDDFGSDSEYDTYAMAFNFFHGGFRDQDVLAWRVALCETSDDTPFFERCQFGSAHDL
                     RGYPVGRYYDDAMYAAQVEYRVPLWKRLGAVAFAGVGGVAESFGDFGSDDTLGAAGVG
                     MRVLASKDQRLNVSIDYAVGRDDSTFYVYIGEAF"
     gene            6027..6836
                     /locus_tag="VIV63_08260"
     CDS             6027..6836
                     /locus_tag="VIV63_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124628.1"
                     /translation="MRSLPLIAGAMSLASLLPSNAPAQEISDDWQFAATIYGWFPDIG
                     GDTSLPPGSSSSIDVDINAILDHLKMTAQGSFSIQKGRWGALTDVVYLDVGDSNSRTR
                     NVEIGGVPLPASVTAAMDFDLKSVFLTLGATYRVTASEEATLDVLLGARLASLKPSLE
                     WEFTGNFGPVTPPPLVGSLEESIEQWDAIVGVRGQFAFGADQKWVVPYHLDVGTGDSD
                     LTWQAMVGLAYGFGWGDLGVAWRYLEYDVGSDGPITDINFSGPAIGATFRW"
     gene            6867..>7146
                     /locus_tag="VIV63_08265"
     CDS             6867..>7146
                     /locus_tag="VIV63_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124629.1"
                     /translation="MNLSIKLMTVTALCSLASVAVAQAPASKPLSTSLGVVVFPAKGQ
                     TPEKQSQDEGECFAWSKQQTGFDPMNPSASAPPPPPAQAPPPQQGGQRV"
BASE COUNT         1288 a         2223 c         2322 g         1313 t
ORIGIN      
        1 acatattgga agttaggcac aactacttat ttaccggcct gcgagttatt caccggtctg
       61 cgagggcgta ctaaggggcg cacgcctgat cgcctggtcc agcgccgcaa tcagcgtgtc
      121 gcgccgcaat ggcttggtga aatacgcgac ggcaccatcg gcgagcacct tttcacgcag
      181 cgacggctga tcgtgcgccg tcacgatgat gatcggcaac gcgaccttgg ccacggatag
      241 cgcccgctgg atgtcgcggc ccgtcaggcc cggcatctgg aagtcgagca cgacgcaatc
      301 cgggatcgcc gacttcagcg actcgaggaa ttcctcgccg cttcggtaga tagagacgtc
      361 gaacgatgac gcccgtaaca ttcgcgcgag cgccaggcgt acggattcct cgtcgtcgac
      421 gacggcaatg tgcggcaggc gatttgccat gcacagattt ggcgtgtacg ccgcgcgggt
      481 cgatagtgga cctttgtcct atgaccccat gcgcgcggtg aaattgaacg tggcgccgcc
      541 gtcggcggcg ttctcggccc agagccggcc gccatgcgct tcgatgatac ttcggcagat
      601 cgcgagcccc atgccgagtc cctgtgtctt ggtggtgacg aacggctgga aaatacgctc
      661 cagatcttcg ctcgcgatgc cggcgccgcg gtcgtggatc gcgcacacca cctcacaact
      721 actgccttcg aaccgcgccg tgatggtgat gcggcgctgg tggacgtcga tgttcgacat
      781 ggcttcgcag gcgttcacca cgaggttcag caccacctgc tgcaattgaa tgcgatcgcc
      841 gaggacgagc ggcagatagg gatcgcagac gacgtcgacc gacacatggc gcgtgagcag
      901 atcgatgcgg gcgaggaaca gcacatcctt gatgacattg gcgagatcga ccggctcgcg
      961 cgagatctcg ccgcgcgtga gcatggcgcg aaggcgctgg atgacttcgg cggcgcgcac
     1021 atcgtcggcg acgatgtccc gcaccatgct gccgatttcg cgcaggtccg cgttcggctt
     1081 cttgagcagc tccagcgcgg cttccgcgtt accgaggatc gacgtcagcg gctgggtcag
     1141 ctcatgcgcg aaggcgccgg aaagctcgcc cagtacggcg gcgcggccga ggtgcacgag
     1201 ctgttgctgt tgcgcgcgtt ccttgccttc ggcctgcttg cggaaggtga cgtcggtcca
     1261 tacgagcacg gcgccacctt ccgggcggcg cagcggctcg atggtgactt cgaaccaggt
     1321 gatgcctccc ggcgcatggg cggcgtattc gagctgatga agccgcgagc ggccggcgat
     1381 gacctccgtg acgcaagtgc cgagccgcgt ggcgacggcg tcgcccttgg cgccaacctc
     1441 gaggcaatgg tgcaagaggt gttcgccgag cttgagattg acgaaaggtg tgccgggaat
     1501 gcgttcggcg agctgccgcc aggcctcgtt ggcatcgacg atgcggccgc tgctgtccag
     1561 tatcacgatc ttgtcctgga tcgacgcgag caccgcgccg tgcagcgcat cgctggcggc
     1621 gcgtgcattc tcgagcgcgc ggcgctcatc gagcgcggct ccgagcagca gcagcggaac
     1681 accggtaacc acgaggaaca gcacgaccga cagggtaccg tgcgcatcgg gctgcgacat
     1741 gaacgggcct tcacccgcca gagctccgct gatggcgacg atgccgaaca acagcgtcgc
     1801 cgccgcgctg ccgctcacgc cgaatcgcgc ggcggcccac agcagcatgg gcatcggcag
     1861 gcacagcatc gccgcggaag ccaccgcgcc ggtctggggc gcgaacagcg cggtgaggct
     1921 ggccgcgacg agaccgagcg cgagcagcga ggcttcgatg acgcgggcga ggtcgatgtg
     1981 ctggcgttgc tgatgcaggc ggtcggacaa gtgcaccatc agcgggacca ccgtcaggat
     2041 ggcgaagcta ttggtgagcg cgcgaaccgt ggcagtgagc cagaaactct tgtcgacgtc
     2101 ggtagtgagg aacagggctg ccatcacgat gctcgtcgtg aagggcgcga ccaggccgcc
     2161 gaagaacacc aggcgccacg actgcaagac ccggtcgaat cgctgatcgc cgggctcgaa
     2221 gtaggtcagt gcgaaggcgc cgatgaacgc cgtggcgcag ttgagcacgt actggatggt
     2281 cacccgggcg aggttcacat cgggaagcgg tatctgggcg gcgagatgcg ccggcaacac
     2341 cgcgcacaga tagaaccacc agaagcggcg gggcacgatc agaagcgcgc ccatcaccag
     2401 ggcattgggg agccacaatg ccgataccgg tgcattgggg aaggcgagcg cttcgccgag
     2461 ttgcgccgtc gcgtagtaac cggccgaaag cgccgtggcg ccgagaacgt gagcggcggc
     2521 gggtgttcct aacaatcctt cgcgggcatg gccgagcggg tgccgcgaaa tggccttccc
     2581 cgtttcggac gactcttgga tggtctgagc ggacattgca ttccctgctg cgccgcgcgc
     2641 agtatgcctt cgcgcggcct cgtccgatcc ccccgacaga tcggaccaag gtcccataca
     2701 caagcggcgt gctgcttttc atagtcatgc cgtgactcac ttcccgcatc gcagtcctgg
     2761 ccgcaagccc ggactcgccc gcttgctccg ctggtccgaa gccaccttct ggatagccgg
     2821 cgcaacgtta tgttcgctgt tcgcgctggc gcaggcggac agtgcgatcg gcagtgcgaa
     2881 tgcactcgaa gatttctccg ccacgctcgt tccgcccgat acgtcgctgt gggccgcggg
     2941 acgcgtgcgc gattaccagg catcgctgaa ggagcccttg caggcggtcg cggtgctcac
     3001 catcccgtcg ctcaaactcg aagtgccggt gttcgatcgc gacgatgacc tggccctcaa
     3061 tcgcggcgcc gggctggtcc ccggcatggg cttgcccgac cagggcggca acatcggcat
     3121 cgccggtcat cgcgacggct atttccgcgc gctcaaggac atcaaggtcg gcgaccgcat
     3181 cgagatccgc acgcacatca aggtgcatcg tttccgcgtg acctccatcg acatcgtcga
     3241 caaggccgac aaccggctgc tcgccgatac cgaactaccg gtggtgacgc tggtcacctg
     3301 ttacccgttt tattttctcg gcagcgcgcc ccagagatac atcgttgccg gtgcatacga
     3361 atggacgagc gaaacctaac aaacggagat agatcatgag acgttccacg tacctgtcat
     3421 tcattggtgt tactgcgctg ctcggtggcg cctcggtgcc cgcgctggcg gccgactgcg
     3481 cttccgcgca gttcagcgac gaggtcctgg cccggttccc caacatccgc gccgtctgtt
     3541 ccgaagtcat cgagaaggat ggccagcaat acgcggtggt gaagggagac ctcgtgcgta
     3601 ccggcaacaa cgcggtgtac atgaagttca agatggccga cggcacgaag agcgatacgc
     3661 gcaagatcga gacgcgcccg gagttccgcg tgaaggtcga cggcaagccg gtcagcgtgc
     3721 gcaatctcgc ggtcggccag gagctcacgg cttacgtgaa ggtttccgat ccggtcatcg
     3781 cgctcgagcc gtacgagtcc gtggcgctcg agacgacccc gctggagtcg gcgcccgcca
     3841 cgatggcttc gaacgagact ggcgacatgc cgcgtaccgc gagcatcgtg ccgggtctcg
     3901 gaacgctcgg cgcgctgctg ctggctttcg ccggcatgct gacgttcttc cggcttcgcc
     3961 gcgagtagtc gagtccggct gatctgctct gtgttagtcg tacggggcgc cgcatgggcg
     4021 gcgccccccg acctgcagtc cattcgcagt taaatcatcg cgaaatgatt gccgtcgcga
     4081 tcgtcacgaa agaactcggg cgcgacgtgg ggccgcaaac ccctccccgg tgcgtactcg
     4141 cgccgcagct cgagtcgtgg cgatcgcgca cggcctgaag gatccgattc aataaaggag
     4201 tatgtgcaat gaaatttact tcgatgatgg tttgggcagc gggtctgtcc gtgttgtcgg
     4261 ccggtgtgtg cgcgcaatcg cgcgacccgg gctgggaatt cggtggcgac gtggtttacc
     4321 agagcagcca ggacgtcgat ttcgacaacg gttcgacgtc cttcgatgac gacatcggag
     4381 tttccctgtt cgcggggtat cgcatgagcc cgcgcctgga gttccagttc ggcctcgact
     4441 ggtcgaacgt cggttacaca ggagatttcg tgcccgagga tttcccgggc atttcggtga
     4501 gcgtggatgc ggacatcgag gcgttcacgc cgttcgccaa ggtcaatttc aattttctcg
     4561 aggggccgat cacgccattc gtgactggca atgtcggttg gagcttcatc gataccaata
     4621 ttcccgacgg tccgccgcag acaggctgct ggtgggatcc ctggtacggg caggtgtgca
     4681 gcaccttcca ggagactcgc agtaccgatt cattcacgta cggactcgga ctcggactcc
     4741 gctgggactt gtcgacgggt tactccttgc ggttcgtcta cgagaagcag tggtacgaca
     4801 tcgatcaggc cgacagcgct ccggacttcg accgattcaa gatcgggatc gtgttcgtct
     4861 actgacgacc gcctcgggcc aggaatgaca tgagatcgtt atgccattcc tgcctcggat
     4921 tgctctcact gtcggcctcg ccgggattcg ccaacgattt cccggtaaat acggataccg
     4981 aactcgttca cgacgagcct tccggacaaa cgaagaagaa agtgaacttc gtgctcgcgc
     5041 cgattccgat cgtgaatccg acgatcggca acgggctcgc gctggccgcg ctgctgctct
     5101 acaagttcga tccgaagtct ccggcgtcga tgacggccgt agccgccggc tacaccgata
     5161 ccgatagctg gggtgtcggc atcttgcagg actccaagtt ttccgaggat cgctggcgcg
     5221 tcaaggcggg tgccgcgctc gcggtcgcgc gatacgacct gtacgtcgaa gagatcgcgc
     5281 cggattttca tttttcgacc gagcaacgcc tgggcggcgt gatggcgcag gcgcttcggc
     5341 gcgtcaccgc gcgcctctac gccggtctgc gttatcagcg cgccaccgtc gagtttccag
     5401 ctcccgatgc ggttcaggac ctgatacccg aggacggtct cgatctcgac atcggtggcc
     5461 tggggctggt ggcggaatgg gatacgcgcg atcacagctt ccagccaggg tccggcagct
     5521 atctcacatt ccgcagcaac ttcgcgcgcg acgatttcgg cagcgactcc gaatacgaca
     5581 cctatgccat ggcattcaat ttctttcacg gcggatttcg cgaccaggac gtgctggcgt
     5641 ggcgcgtggc gctgtgcgag acgagcgacg acacgccgtt cttcgagcgt tgccagttcg
     5701 gttcggcgca cgacctgcga ggttatcccg tcggccgtta ctacgacgat gcgatgtatg
     5761 ccgctcaggt cgagtatcgc gtgccgctgt ggaagcggct gggtgcggtc gccttcgcgg
     5821 gcgtgggcgg tgtcgccgaa tctttcggcg acttcggatc ggatgacacg ctgggcgccg
     5881 ctggcgtcgg catgcgtgtg ctcgcttcga aggatcagcg cctgaacgtg agcatcgact
     5941 acgcggtcgg tcgcgatgac tccacgttct acgtgtacat cggcgaagcg ttttgatttt
     6001 caggatggag aagaggacat gaggacatga gatccttgcc attgattgcc ggtgcaatga
     6061 gtcttgcatc gttgctgcct tcgaacgccc cggcgcagga aatttccgat gactggcaat
     6121 tcgccgccac gatctacggc tggtttcccg acatcggcgg cgacaccagt ctgccgcccg
     6181 gcagctccag cagcattgac gtcgacatca acgcgatcct cgatcacctg aagatgaccg
     6241 cccaggggtc gttcagcatc cagaagggcc gatggggcgc gctcacggat gtggtgtatc
     6301 tcgacgtcgg agattcgaat tcacgcacgc gcaacgtcga gatcggaggt gtgccattgc
     6361 cggcgagcgt cacggcggcg atggacttcg acctcaaaag tgtcttcctg actctcgggg
     6421 cgacctatcg cgtgaccgcc agcgaagagg ccacgctcga tgtgttgtta ggcgcacggc
     6481 tcgccagtct gaaaccgtcc ctcgagtggg agttcaccgg caacttcgga ccggtgacgc
     6541 caccgccgct ggtcggcagc ctcgaggaat ccatcgaaca atgggacgcg atcgtcggcg
     6601 tccggggtca attcgcgttc ggtgccgatc aaaagtgggt ggtgccttac cacctcgacg
     6661 tgggtaccgg cgattccgac ctcacgtggc aggcgatggt gggcctcgcc tatggattcg
     6721 ggtggggaga cctcggcgtc gcgtggcgct atctcgagta cgacgtgggt tccgacggac
     6781 ccatcaccga catcaacttc agtggcccgg cgatcggcgc cacattccgc tggtaacacg
     6841 gcggatcaac tcccaaggag ctcgcaatga atctctctat caaactcatg accgtgacgg
     6901 cgttgtgctc gctcgccagc gtcgccgttg cccaggcacc ggcgtccaag ccgctgtcga
     6961 cctcactggg cgtcgtggtg tttcccgcga agggacaaac acccgagaag cagagccagg
     7021 acgaaggcga atgtttcgcg tggtccaagc agcagacggg cttcgatccc atgaacccga
     7081 gcgcgtccgc accaccgccg ccaccggccc aggcgccgcc gccgcaacag ggcgggcagc
     7141 gcgtgc
//
LOCUS       DATDEU010000347         2390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_794035, whole genome shotgun sequence.
ACCESSION   DATDEU010000347 DATDEU010000000
VERSION     DATDEU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794035"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VIV63_08270"
     CDS             complement(<1..182)
                     /locus_tag="VIV63_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124630.1"
                     /translation="MTSEITVTGTTLISLMPALPEIYLTAAICVLLLADVFFGDKNRG
                     LAPTLTLLILAVGAGLT"
     gene            complement(179..1696)
                     /locus_tag="VIV63_08275"
     CDS             complement(179..1696)
                     /locus_tag="VIV63_08275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY6124631.1"
                     /translation="MQEFPLLSFLTWLPIAGGVLVLLFGDRGIRAGRWVALGIALATF
                     GASLPLYCGFDSSSFAFQFTEQLPWIPAFHATYYLGVDGISLPLILLTTFITIPVVIA
                     AWTVIETRPAQYYAAFLIMEGLMIGVFSALDGLLFYFFWEAMLIPMFLIIGVWGGPRR
                     VYATLKFFLYTFLGSVFMLVALIYMLMKTSDFSIAAFHALPLTAAEQMWIFFAFLAAF
                     AVKIPMVPVHTWLPDAHVEAPTGGSVVLAAIMLKMGGYGFLRFALPITPDASREFDWL
                     LILLSLIAVVYIGFVALVQTDMKKLIAYSSIAHMGFVTLGSFLVYDIIDGTGTVQGAG
                     MGIDGAMVQMVSHGLISGALFLCVGVMYDRVHSREISAYGGVINTMPKFAAFMVFFAL
                     ANAGLPGTSGFVGEFLVIIAAFKANFWYAFLAAVTLILGAAYTLWLVKRVIFGEVANH
                     HVAELQDLNYREFIILGVLAVAVLVVGLWPAPLLEVMQATVQHLVQQIAASKLSL"
     gene            complement(1732..>2390)
                     /locus_tag="VIV63_08280"
     CDS             complement(1732..>2390)
                     /locus_tag="VIV63_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124632.1"
                     /translation="HDDHSHDDHAHAHASHDDHGHGHHGGPPLREMPTMTWPLIALAI
                     PSVIIGAWTFKALLFGGGFGESIYFNSEHHEALHEIGESVGDWLHFGLHAVTNPVFWI
                     MALGVFTSWALYIWKPHLPGLIDSKLKPIRVVLENKYFFDWFNENVLAAGSRLLGKVF
                     WKVGDQLIIDKIAVDGSAHTVGFVAGIVRRVQSGFLYAYAFWMVIGLAVMLGWFLTRA
                     "
BASE COUNT          535 a          711 c          779 g          365 t
ORIGIN      
        1 gtgaggccgg caccgaccgc taagatgagc agcgtgagcg tgggcgccaa cccacggttc
       61 ttgtcgccaa agaacacgtc ggccagcagc agcacgcaaa tggcggccgt gagatagatc
      121 tccggcaacg cgggcatcag gcttatcagg gtcgtccccg tcactgtgat ttcggaagtc
      181 atagggacaa cttgctggcg gcgatctgtt ggacgagatg ctggacggtg gcctgcatga
      241 cttcgagcag cggcgcgggc cacaggccca cgaccagcac ggccacggcc agaacaccca
      301 ggatgatgaa ttcgcggtag ttgagatctt gcagctcggc gacgtggtgg ttcgccactt
      361 caccgaagat gacgcgcttg accagccaca acgtgtaggc cgcgcccagg atcagcgtga
      421 ccgcggcgag gaaggcgtac cagaagttgg ccttgaacgc ggcgatgatg acgaggaact
      481 cgccgacgaa acccgaggtg cccggcaggc cggcgttcgc cagcgcgaag aacaccatga
      541 atgcggcgaa cttcggcatg gtgttgatca cgccgccgta ggcgctgatc tcgcgcgagt
      601 gcacgcggtc gtacatgacg cccacgcaca ggaacaacgc gccggagatg aggccgtgcg
      661 acaccatctg aaccatggcg ccgtcgatgc ccatgcccgc accctgcacg gtgcccgtgc
      721 cgtcgatgat gtcgtacacg aggaaagagc ccagcgtgac gaagcccatg tgcgcgatcg
      781 acgaataggc gatgagcttc ttcatgtcgg tctgcacgag cgcgacgaag ccgatgtaca
      841 ccaccgcgat gagcgagagc aggatcagca gccagtcgaa ttctctcgaa gcgtcgggcg
      901 taataggtag cgcgaaacgc aggaaaccgt atccgcccat cttcagcatg atcgcggcca
      961 gcaccaccga gccgccggtc ggcgcctcga cgtgcgcatc gggcaaccac gtgtgcaccg
     1021 gcaccatcgg aatcttgacg gcgaaggccg cgaggaacgc gaagaagatc cacatctgct
     1081 cggcggccgt cagcggcagc gcgtggaacg ccgcgatgct gaagtcgctg gtcttcatca
     1141 gcatgtagat gagcgcgacc agcatgaaca cggaacccag gaacgtgtac aggaagaact
     1201 tgagcgtggc gtacacgcgc cgcgggccgc cccagacacc gatgatgagg aacatcggga
     1261 tcagcatggc ttcccagaag aagtagaaca gcagaccgtc gagcgcggag aacacgccga
     1321 tcatcaggcc ttccatgatg aggaaggcgg cgtagtactg cgccgggcgc gtctcgatca
     1381 cggtccacgc ggcgatgacc acgggaatgg tgatgaacgt ggtcagcagg atgagcggca
     1441 gcgagatgcc atcgacaccc aggtagtaag tggcgtggaa cgccggaatc cacggcaact
     1501 gttccgtgaa ctggaatgcg aagctcgacg aatcgaatcc gcagtacagc ggcagcgagg
     1561 caccgaacgt ggcaagcgcg atgccgagcg ccacccagcg ccctgccctg atgccgcgat
     1621 cgccgaacaa cagcacgagc acgccgcccg cgatcgggag ccaggtcagg aaagaaagaa
     1681 gcggaaactc ctgcatgtgc cttttagtct cggacttggg ttgttgttag atcaggcgcg
     1741 ggtcaggaac cagccgagca tgacggcgag tccgatgacc atccagaacg cataggcgta
     1801 gagaaaaccg ctttgcacgc ggcgcacgat gccggcgacg aagccgaccg tgtgcgcgga
     1861 gccatcgacc gcgatcttgt cgatgatgag ctgatcgccc accttccaga aaaccttgcc
     1921 gaggagccgg ctgccggcgg cgagcacgtt ttcgttgaac cagtcgaaga agtacttgtt
     1981 ctcgagcacg acgcgaatgg gcttgagctt cgagtcgatg aggcccggca ggtgcggttt
     2041 ccagatgtac agcgcccagg acgtgaacac accgagagcc atgatccaga acaccgggtt
     2101 cgtcaccgcg tgaaggccga aatgcagcca gtcgcctacg ctctcgccaa tctcgtgaag
     2161 cgcttcgtgg tgctcggaat tgaagtagat ggactcgccg aatccgccgc cgaacagcag
     2221 cgccttgaag gtccaggcgc caatgattac cgatgggatg gccagcgcga tgagcggcca
     2281 ggtcatcgtc ggcatttccc tgagcggcgg accgccatga tggccgtggc catggtcgtc
     2341 atgcgaagcg tgggcgtgcg catggtcgtc gtgcgaatga tcgtcatgcg
//
LOCUS       DATDEU010000348         3090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1814567, whole genome shotgun sequence.
ACCESSION   DATDEU010000348 DATDEU010000000
VERSION     DATDEU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814567"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VIV63_08285"
     CDS             <1..752
                     /locus_tag="VIV63_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124633.1"
                     /translation="DYPPDHFRLLALRSREAAEDRPWVFVTGGVHGYETSGVHGALQF
                     LEQRAADYGEEINLLAVPCVSPWAYERILRWNANAVDPNRSFGDGGAAGESAALLRLI
                     APLRGRVLLHIDLHETTDSDESEFRPALAARDGKPFEPDVTPDGFYLVDDSENPQPEF
                     QRAIIDAVSQVTHIAPADGKNEIIGSEVVAPGVIRYPLRKLGLCAGITGARYTTTTEV
                     YPDSPRATPEQCNAAQVAATCAAIEFVLRSK"
     gene            complement(755..1111)
                     /locus_tag="VIV63_08290"
     CDS             complement(755..1111)
                     /locus_tag="VIV63_08290"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY6124634.1"
                     /translation="MMLMKMKEATENMVPPTEEAMLAMHNYNEALIAAGILKDKVYGG
                     LMPTSFGKLLKYEGKNLTVIDGPFTETKEVVAGFALWEVKSIDEALEWARKCPVMMDC
                     EAELRPVYCPEMFDPK"
     gene            1217..2479
                     /locus_tag="VIV63_08295"
     CDS             1217..2479
                     /locus_tag="VIV63_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6124635.1"
                     /translation="MSVTDRPRHEMHDPAHIDLALSSARPKAVAALLRYFRDLDTAEE
                     AFQEACLRALQTWPQKGFPVSPTAWLVTVGRNAALDTKRRNRSIEPLADVDSIADPGS
                     ESTPEGLDESHYRDDILRLLFVCGHPTLPATQQIALALRIVSGLTVEQIAAAFLVTPA
                     AMEQRITRAKRTIANVGVEFETPGAAERRTRLAAVSTMIYLVFNEGYGSTPESASSRA
                     ALAAEAIRLGRLLLSLFPADPEVHGLLALMLLQQSRAPARFDANGEIVLIDEQDRSLW
                     NRTQIDEGRLLLERSAIYQNPGPYQLQAAIAALHVHAARPEDTAWKEIDSLYQLLESL
                     QPSPVTTLNRAVAVWKLRGAEVALQMIDPLAGELDAYFYFHGLRGALLKELNRVDEAR
                     EAMNRAIALAKSVAEAKHIRRELDRLGR"
     gene            complement(2499..>3090)
                     /locus_tag="VIV63_08300"
     CDS             complement(2499..>3090)
                     /locus_tag="VIV63_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124636.1"
                     /translation="SDRCSIVMGETSVRPAQRPFRAQSDHDLAAAFESALAAGDGVAG
                     AHCIHERWMRGHFAAHIEAALDLLWKRAGPSIPDWLPMRYVSWLPVAYDVAARFTAKG
                     RGRSNIYLVLLDYADRRGDDHGVYVGMSRYSPAARFDQHKAGIRAAGSVLKRGIEVLT
                     GPTLHLQHIKRGDAARIEAELALALADAGIHVEGGH"
BASE COUNT          566 a         1003 c          955 g          566 t
ORIGIN      
        1 tcgactatcc accggaccac tttcgactac ttgcgttgcg cagccgcgag gccgcagaag
       61 atcgaccgtg ggtgttcgtc acgggcggcg tgcacggcta cgagacgagc ggcgtgcacg
      121 gcgcactgca gttcctcgag caacgcgcgg cggactacgg agaggaaatc aatcttctcg
      181 cggtgccttg cgtgagcccc tgggcttacg agcgaattct tcgctggaac gcgaatgccg
      241 tcgatccgaa ccgatcgttc ggcgatggcg gcgccgcggg cgaatcggcg gccctgctgc
      301 gtctgattgc gcctttgcgc ggccgcgtgc tgctccacat cgatcttcat gagacgaccg
      361 actccgacga aagcgaattc cgccccgcgc tcgccgcgcg cgatggaaaa ccgttcgagc
      421 cggacgtcac accggatggg ttctatctcg tcgacgacag cgagaatccg cagcccgaat
      481 ttcagcgcgc gatcatcgat gcggtgtcgc aggtcacgca catcgctccc gcggacggca
      541 agaacgagat catcggctct gaagtcgtgg cgcccggcgt gatccggtat ccgcttcgca
      601 agctcggcct gtgcgccggc atcaccggag cgcgctacac gaccaccacc gaggtctatc
      661 cagacagccc tcgcgcgact cccgagcagt gcaatgccgc gcaggtcgcg gcgacgtgcg
      721 cggcgatcga gttcgtgttg cgatcgaaat gacgctattt ggggtcgaac atttccgggc
      781 aatacacggg acgtagttcg gcttcgcagt ccatcatcac gggacacttc ctcgcccact
      841 cgagcgcctc gtcgatggac ttgacctccc acagcgcgaa gcctgcgacc acttctttgg
      901 tctcggtaaa tggcccgtcg atcaccgtga gattcttgcc ctcgtatttg agcagtttgc
      961 cgaaactggt cggcatgagg ccgccgtaga ccttgtcttt caggatgcca gcggcgatga
     1021 gagcctcgtt gtaattgtgc atggccagca tcgcctcttc agtgggcggg accatgtttt
     1081 cagtggcttc cttcattttc atcaacatca tgactttcat cgaacggctc cgggttacgg
     1141 tggattcagc ttcaaggacg cccgtaatgt ccccgtgccg acaacggcat gaaaatatat
     1201 tggtagggtg tctgccatga gcgtaaccga tcgtcctcgg cacgagatgc acgatcccgc
     1261 gcacatcgac ctggcgcttt catcggcgcg accgaaggct gttgccgccc tcctgcggta
     1321 cttccgcgac ctggacacgg ccgaggaggc tttccaggaa gcctgtcttc gcgccctcca
     1381 gacctggccg cagaaagggt tccccgtgag cccgacagcc tggctcgtga cagtcgggcg
     1441 gaatgcggct ttggacacca agcgtcgcaa cagatccatc gaacctctgg cggacgtgga
     1501 ttccattgcg gatcccggca gcgaatcgac gccagagggc ctcgatgaaa gccactatcg
     1561 cgacgacatc ctgcggctgc tgttcgtgtg cggtcatccc accctgccgg caactcagca
     1621 gatcgcgctc gcgctgcgaa tcgtatccgg actgaccgtg gagcagattg ccgcggcgtt
     1681 cctcgtgaca cccgccgcga tggagcagcg aatcacccgc gccaagcgga ccatcgcgaa
     1741 cgtcggcgtc gaattcgaaa cccccggcgc cgccgagagg cgcacgcgac tcgcggcggt
     1801 ctcgacgatg atctatctgg tcttcaacga aggatacgga agcaccccgg aatccgcttc
     1861 gtcgcgcgcg gcgcttgcgg ctgaggccat ccgcctggga cggctgcttt tgagcctctt
     1921 tccggccgat ccggaggttc acggactcct ggcgctgatg ttgcttcagc agtctcgcgc
     1981 acccgcgcgc ttcgacgcga acggcgagat cgtgctgatc gacgagcagg accgcagcct
     2041 ctggaatcgt acgcagatcg acgaaggccg attgttgctg gaaaggtccg ctatctacca
     2101 gaacccgggg ccgtaccagc ttcaggcggc catcgctgct ctgcacgtgc acgcggcgag
     2161 acccgaagac accgcatgga aggaaatcga ttcgctgtac cagctactcg aatcgctgca
     2221 gccatcaccc gtgacgacgc tcaatcgcgc cgtcgcggtc tggaagttgc gaggcgcgga
     2281 ggttgcgctg cagatgatcg acccgctggc cggtgagctc gacgcctatt tctattttca
     2341 cggactgcgc ggcgcgctgc tcaaggaact gaaccgtgtc gacgaggcca gggaagccat
     2401 gaatcgtgcc atcgcgcttg cgaaatccgt cgcggaagcg aagcacatcc gacgagagct
     2461 ggatcgtctc ggccgttgag cctgctcaac tcctgagctc agtggccacc ttcgacgtga
     2521 atgccggcat ccgcgagcgc gagagccagt tctgcttcga tgcgcgccgc gtcaccgcgc
     2581 ttgatgtgct gaagatgaag cgtcgggcca gtcagcactt cgatgccgcg cttcagcacg
     2641 ctcccggcag cgcggattcc ggccttgtgc tgatcgaagc gcgccgccgg ggaatatcgg
     2701 ctcattccga catacacgcc gtggtcatcg ccgcgccgat cggcatagtc gagcagcacc
     2761 agatagatgt tcgagcggcc gcggcccttc gcggtgaagc gcgcggcgac gtcgtacgcc
     2821 acgggtagcc agctgacgta gcgcatcgga agccagtccg ggatcgatgg ccccgcacgc
     2881 ttccacagaa gatcgagcgc ggcctcgatg tgcgccgcga agtggccacg catccagcgt
     2941 tcatgaatgc agtgcgcgcc cgccacgccg tcgccggcgg caagcgcgga ctcaaacgcc
     3001 gccgcgaggt cgtggtccga ctgagcgcgg aacggtcgtt gagcgggtcg tacagaagtt
     3061 tcacccatca cgatgctgca tcggtccgat
//
LOCUS       DATDEU010000349         4147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1523367, whole genome shotgun sequence.
ACCESSION   DATDEU010000349 DATDEU010000000
VERSION     DATDEU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523367"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VIV63_08305"
     CDS             <1..705
                     /locus_tag="VIV63_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6124637.1"
                     /translation="WAPNDKTLFWLAGSRAIRAPTPFDTDVQEFVGGQLFVLGDPDFK
                     PEKVTAYEFGYRGNPDAALSLSASVFYNEYDDLRTIEITPVVFLPLRWGNLMEGSAYG
                     FEAWADFQITSWWRLSPGVRTLHKRLEFSDGASGITGVRQAGNDPSSRYYLKSSMDFR
                     HFTLDVMVRNVSSLPDPALDEYSEMNARFAWRASERLELAVKGFNLLNEIHREYPDPS
                     GFAIHRSVMAELRYNY"
     gene            785..1243
                     /locus_tag="VIV63_08310"
     CDS             785..1243
                     /locus_tag="VIV63_08310"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEY6124638.1"
                     /translation="MSAHLQERRATQDRRRSVLRALWHGNFARRRHAPRRSLERHFAV
                     TDWFHAQWLVVGIGILLLCAADAILTLTLIARGASEINPLMDPLVRGSGHSFAYWKIG
                     LTAMGVLVLTLLARVRLWGRAVGTILYVVLGAYVVLVAYELFLLRKIPLD"
     gene            1335..>4147
                     /locus_tag="VIV63_08315"
     CDS             1335..>4147
                     /locus_tag="VIV63_08315"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY6124639.1"
                     /translation="MAPSLRELTASTPLSGGNAPYVEALYEQFLADPQSVEPAWRDYF
                     HHLQDGLKLRDGAGAEQVHSTVQAGIVQRAAKPRMATAPGMLSTESAAKQGAVSRLVQ
                     IYANRGHLIANLDPLGLEKRPRPRVLELGYFGLTDADLDTEFLTGSRPNELAPRLKLR
                     EIIAQLEHAYCSTIGAEFAHVSNTEERLWLQDQYLLGRLRGKLTTEEQKNILWQLSAA
                     EGLERYLHTKYVGQKRFSLEGGDSLIPLMDDLIQRGGEQGVEECVIGMAHRGRLNVLV
                     NVLGKSPSVLFNEFEGKYDTAHMQGSGDVKYHKGFSSDLRTPGGNVHVALAFNPSHLE
                     VVNPVVEGSVRARQERRGDTTGSRVLPILIHGDAAFAGQGVVMETLQLSQARAFFTGG
                     SVHIIVNNQVGFTTSDPHDARSTMYCSDVAKMLETPIFHVNSDDPDAVLFVTRLALAY
                     RQRFHKDVVIDLVCYRRHGHNEADEPSATQPMMYQIIRKHLTTRKLYADKLVAMGVIT
                     DAEAAAMTESYRQGLDEGRPQARASLGMIGNKYTVDWSKYLGNDWSERVKGGNDMARL
                     KSLGARIVKVPENFALHPRVAQVMANRVKMLAGELPLDWGCAETLAYASILEEGNAIR
                     FTGQDAGRGTFFHRHAVLHEQSTGARYIPLQHIADRQPSFQIIDSVLSEEAVLGFEYG
                     YSTTDPSALVIWEGQFGDFVNGAQVIIDQFISSGEAKWGRLCGLTLFLPHGYEGQGPE
                     HSSARLERFLQLCAENNMQVCVPSTPAQMYHMIRRQILRSLRKPLIVMTPKSLLRHEL
                     SVSSLEDLANGEFATVIDEIDKLTPANVRRVVLSSGKVYFDLLKARRATSNNEVALVR
                     VEQLYPFPVPEMEAVLARYPNAREVVWCQEEPQNQGAWYQIRHRLATLVGKRELLYAG
                     RAPAAAPSTGITKIHETEQRGLI"
BASE COUNT          749 a         1370 c         1257 g          771 t
ORIGIN      
        1 tgggcgccga atgacaagac gctgttctgg ctcgccggtt cccgcgccat ccgcgcaccc
       61 acgccgttcg acaccgacgt gcaggagttc gtcggtggcc agctcttcgt actcggtgat
      121 ccggatttca agcccgagaa agtgacggcg tacgagtttg gctatcgagg caacccggat
      181 gccgcgttgt cgctgtccgc cagcgtgttc tacaacgagt acgacgatct gcgcaccatc
      241 gagatcacgc cggtcgtgtt cctgccgctg cgctggggaa atctcatgga aggcagcgcc
      301 tacggcttcg aggcgtgggc cgatttccag atcacttcgt ggtggcgtct ttcgcccggc
      361 gttcgcacgc tgcacaagcg cctcgagttc agcgacggcg cgtccggtat cacgggagtg
      421 cgccaggccg gcaacgatcc ctcttcacgt tactacctga agtcatcgat ggattttcgt
      481 cacttcacgc tcgacgtcat ggtgaggaac gtgagttcgc tgcccgaccc cgcgctcgac
      541 gaatattccg aaatgaatgc ccgcttcgcc tggcgcgcca gcgaacggct cgagctcgcc
      601 gtcaagggct tcaatctact taacgaaata caccgcgagt atcccgatcc gtcaggcttc
      661 gcgatccatc gcagcgtcat ggcggagctg cgctacaact actgagagaa atgggacttg
      721 gctcacacgc gatagaggcg ctcgggcgtg tacgggcttc gcgtgctcat agaatgatcg
      781 gcagatgtca gcacacctgc aggaacgacg cgccactcag gatcgacgcc gttccgttct
      841 tcgcgccctg tggcacggaa atttcgcccg gcgccgccat gctccgcgtc gaagcctgga
      901 acgccatttc gccgtcaccg actggtttca cgcgcaatgg ctcgtggtgg gcatcggaat
      961 cctgcttctc tgcgccgccg acgcgattct cacgctgacg ctcatcgcgc gcggcgcttc
     1021 cgagatcaat ccgctcatgg acccgctggt ccgaggttcg gggcacagct tcgcgtactg
     1081 gaagatcggg ttgaccgcga tgggggtgct cgtgctcacc ctgttggccc gtgttcgttt
     1141 atggggcagg gcggttggaa ccatccttta tgtggttctc ggggcttatg tggtcctggt
     1201 ggcttacgaa ctatttcttt tacgtaagat ccccctagat taggcactgg tcgcctttcg
     1261 gctgcttccg gcatccgcta acataccgcc cccttatcgc tccaggacct tgagtccata
     1321 ggattttttc tcgtatggcc cccagccttc gcgagcttac ggcttccaca ccgctgtctg
     1381 gtggcaacgc tccttacgtc gaagctctct atgagcagtt cctcgctgat ccccagagtg
     1441 tcgagcccgc gtggcgcgac tactttcacc accttcagga cggcctgaag ctgcgggacg
     1501 gcgccggcgc cgagcaggtg cactccaccg tgcaggcggg catcgtgcag cgtgcggcca
     1561 aaccgcgcat ggccacggcg cctggcatgc tttccacgga atccgccgcc aagcagggcg
     1621 ccgtctcgcg cctggtgcag atctatgcaa accgcggcca cctcatcgcc aatctggatc
     1681 cgctgggtct cgagaagcgt ccgcgtccgc gggttctcga acttggttac ttcggcctga
     1741 ccgacgccga tctcgatacc gaattcctca ccggttcgcg ccccaatgag ctggctccgc
     1801 ggctcaagct gcgcgaaatc atcgcccagc tcgaacacgc ctactgcagc accatcggcg
     1861 ccgagttcgc gcacgtatcg aacaccgaag agcgcctgtg gctgcaggac cagtacctgc
     1921 tcggccgcct gcgcggcaag ctgactaccg aagaacaaaa gaacatcctg tggcagctct
     1981 cggctgccga aggtctcgag cgttacctgc acaccaagta cgtcggccag aagcgcttct
     2041 cgctcgaagg cggcgactcg ctcatcccgc tgatggacga cctcatccag cggggcggcg
     2101 agcagggcgt cgaagagtgc gtcatcggca tggcccatcg cggccgcctc aacgtgctcg
     2161 tcaacgtgct cggcaagtct ccgtcggtgc tgttcaacga attcgaaggc aagtacgaca
     2221 ccgcgcacat gcagggctcg ggcgacgtga agtaccacaa gggcttctcg tccgatctgc
     2281 gtacgccggg cggcaacgtt cacgtggcgc tggcgttcaa cccctcgcat ctcgaagtag
     2341 tcaatcccgt ggtcgaaggt tcggtgcgcg cacgccagga gcgccgcggc gacaccaccg
     2401 gttcgcgtgt gttgcccatc ctcatccacg gcgacgcggc cttcgcggga cagggcgtgg
     2461 tcatggagac gctgcagctt tcgcaggctc gcgcgttctt cactggcggc agcgtccaca
     2521 tcatcgtgaa caaccaggtg ggtttcacca cctccgatcc gcacgacgcg cgttcgacca
     2581 tgtattgctc ggacgtcgcc aagatgctcg agacgccgat cttccacgtg aacagcgacg
     2641 acccggacgc ggtgttgttc gtgacgcgcc tggcgctcgc gtatcgccag cgtttccaca
     2701 aggacgtggt catcgacctg gtctgttatc gccgccacgg tcacaacgag gcggacgagc
     2761 catcggccac gcagccgatg atgtaccaga tcattcgcaa gcacctgacc acgcgcaagc
     2821 tctacgcgga caagctcgtg gccatgggcg tcatcaccga cgccgaagcc gcggcgatga
     2881 ccgagtctta ccgccagggt ctcgacgaag gccggccgca ggcgcgcgca tcgctcggca
     2941 tgatcggcaa caagtacacg gtcgactgga gcaagtacct cggcaacgac tggtccgagc
     3001 gcgtgaaggg tggcaacgac atggcccgcc taaagtcatt gggcgcgcgc atcgtgaagg
     3061 tgccggaaaa cttcgcgctg catccgcgcg tggcgcaggt catggccaat cgcgtgaaga
     3121 tgctcgcggg cgaattgccg ctcgactggg gctgcgcgga aacgctggcc tacgcgagca
     3181 tcctcgaaga aggcaatgcc atccgcttca cgggccagga cgccggccgt ggcacgttct
     3241 tccatcgcca cgcggtactg cacgagcagt ccaccggtgc gcgttacatc ccgctgcagc
     3301 acatcgcgga tcgccagcct tcattccaga tcatcgactc ggtgttgtcg gaagaagccg
     3361 tgctcggttt cgaatacggt tactccacca ccgatccgtc ggcgctggtc atctgggaag
     3421 gccagttcgg cgacttcgtg aacggtgcgc aggtcatcat cgaccagttc atcagctcgg
     3481 gcgaagcgaa gtggggccgg ctctgcggtc tcacgctgtt cctgccgcac ggttacgaag
     3541 gccagggccc ggaacattcc tccgcgcgcc tcgagcgctt cctgcaactc tgcgccgaga
     3601 acaacatgca ggtgtgcgtg ccgtcgacgc ccgcgcagat gtatcacatg atccgaaggc
     3661 agatcctgcg cagcctgcgc aagccgctca tcgtcatgac gccgaagagc ctgctgcgtc
     3721 acgaactgtc ggtgtcctcg ctcgaagacc tggccaacgg cgagttcgcc acggtcatcg
     3781 acgaaatcga caagctgacc ccggccaacg tgcgccgcgt ggtgctgtcg tccggcaagg
     3841 tgtacttcga cctgctcaag gcgcgccgcg ccaccagcaa caatgaagtg gcgctggtgc
     3901 gtgtcgagca gctctatcca ttcccggtgc cggaaatgga agcggtgctc gcccggtatc
     3961 cgaacgcgcg cgaagtggtg tggtgccagg aagagccgca gaatcagggc gcctggtacc
     4021 agatccgcca tcgcctggcc acgctcgtcg gcaaacgcga actgctgtac gcgggccgcg
     4081 cgccggccgc ggcgccgtcc acgggcatca ccaagatcca cgaaaccgaa cagcgcgggc
     4141 tcatcga
//
LOCUS       DATDEU010000350         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1814770, whole genome shotgun sequence.
ACCESSION   DATDEU010000350 DATDEU010000000
VERSION     DATDEU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814770"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VIV63_08320"
     CDS             <1..1386
                     /locus_tag="VIV63_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6124640.1"
                     /translation="DLESPKRIDRLKVAASDFVATAENGTEVGIVSFASDAETGSGRV
                     SVPIAALGANRSAWSDEIDDMAPDSMTNIGAGLQKAKDMIVAAGGVTANTYVVLMTDG
                     VNNEPGGGATAQADLDAKIADLLASGIPVFVTCTGGDLGLQSQCAEIASGTNGTYSDS
                     ADAARLPQNFVDFHERITGHQGVGTIDSKLKSIAGGHGVKFLVDQYSESASFSLLWDN
                     KASSANAVLVDPSGTRHQTRAIPQGRYARIRNPLAGEWTMIINTDGPDSRAIARAYVQ
                     NRISNFVTSTRNPTQEPGEEIYVYASARSMGGGLSEPGTKLVANVTLPGGGTDTLELF
                     DNGRDPGGHGDDMAGDGIYTGVYTNTSQKGAYGFSVTGAIDKWHQGSDPHKQNPTIMS
                     PRFDRDVRVSAAVGSPGDVVTNPEDERPKPGKVDVNRWILILILILLILLIIMQWLCC
                     RKRNPDPVVIR"
     gene            1431..1790
                     /gene="yecR"
                     /locus_tag="VIV63_08325"
     CDS             1431..1790
                     /gene="yecR"
                     /locus_tag="VIV63_08325"
                     /inference="COORDINATES: protein motif:HMM:NF025358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HEY6124641.1"
                     /translation="MIRFTPTARFLAAAALLLSLAACATTSGQKWAVSGGDRSDGVVR
                     ISYEYPEFHQPTVSDEQAMKLALGRCEGWGFDDAQPIAGQVRKCANMDGTNCNLWTVT
                     REYQCKTSGAFASNFAK"
     gene            complement(1791..>3019)
                     /locus_tag="VIV63_08330"
     CDS             complement(1791..>3019)
                     /locus_tag="VIV63_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY6124642.1"
                     /translation="NYPWAFYVTNNKTLDEALYADSQRWSNLGRKWFASGRPYREIDA
                     VDGTPNPWLQRWLMHPAYDKYWQSMVPNKSDFGRIDIPILNITGYYDDAQISALQYFN
                     QHAATGRNPEHYLVIGPFDHFGTNARQKPEVLREYTIDPVAQIDSQELKLQFMDYVLR
                     GKPKPALLRNIVNYEVMGANVWRHAPSLGSVHGGVPMRLYFSSQKNQGVYSLANRQPP
                     SDSVVTHEVDLADRVKFHNFHAYPMPIVQGPLSYVTESIFVSEPFETPTVISGSFTGE
                     LFAIINKKDFDLGVTVYEAMPDGKLFHLAYALHRASYSEDPGKRNLLKPGKLEHVKFE
                     TTMTSRQMLPGSRLFVLVDANKNPMAQVNYGTGKDVSDESVKDAGEPLKIEIRSGSFF
                     QVPLDNSFSQKPASPQ"
BASE COUNT          568 a          943 c          960 g          548 t
ORIGIN      
        1 gatctcgagt cgccgaagcg catcgatcgc ctcaaggtgg cggccagcga tttcgtggcc
       61 acggccgaga acggcacgga agtcggcatc gtctcgttcg cgtccgacgc ggagaccgga
      121 agcggccgcg tgagtgtgcc catcgcggca ctcggcgcga atcgctccgc gtggagcgac
      181 gagatcgacg acatggcacc ggactcgatg accaacatcg gcgccggtct gcagaaggcc
      241 aaggacatga tcgtcgcggc gggtggcgtc accgcgaaca cgtatgtcgt gctgatgacc
      301 gacggcgtca acaacgaacc gggcggcggc gcgaccgcgc aggccgacct cgacgcaaag
      361 atcgcggacc tgctcgcctc gggcattccg gtgttcgtca cgtgcacggg cggagatctc
      421 ggcctgcaat cgcagtgcgc ggaaatcgcc agcggcacca acggcacgta ctcggattca
      481 gcggacgccg cgcggctgcc gcagaacttc gtcgacttcc acgagcgcat cacgggccac
      541 cagggcgtgg gcacgatcga cagcaaactg aagagtattg ccggaggtca cggggtgaaa
      601 ttcctcgtcg accagtacag cgagtccgcc agtttcagcc tgctgtggga caacaaggca
      661 tcgagcgcga acgcggtgct ggtcgacccg agcggcacgc gtcatcagac gcgcgccatt
      721 ccgcaaggcc gctacgcgcg catccgcaat ccgctggccg gtgaatggac catgatcatc
      781 aataccgatg gtcccgacag ccgcgcgatc gcgcgtgcgt acgtgcagaa ccgcatcagc
      841 aacttcgtga cgtcgacgcg caatcccacg caggagccgg gcgaagaaat ctatgtctac
      901 gcgtccgcgc gcagcatggg cggcggcttg agcgagccgg gcacgaaact cgtcgcgaac
      961 gtcacgttgc cgggcggcgg caccgacacc ctggagctgt tcgacaacgg tcgcgatccg
     1021 ggcggtcatg gcgacgacat ggcgggcgat ggcatctaca ccggcgtgta caccaacacc
     1081 tcgcagaagg gcgcttacgg attcagcgtg acgggcgcca tcgacaagtg gcaccagggc
     1141 tccgatccgc acaaacagaa ccccaccatc atgagtccgc gcttcgatcg cgatgtgcgc
     1201 gtgtcggcgg ccgtgggttc gccgggagat gtcgtcacca accccgagga cgagcggccc
     1261 aaacccggca aggtggacgt caaccgctgg atcctcatcc tcattctgat cctgctgatt
     1321 ctcctgatca tcatgcagtg gctgtgttgc cgtaagcgca acccggaccc ggtggtcatt
     1381 cggtagccga ttcaggatag gttaaggagt gggggcgcat tctggccccc atgatccgat
     1441 tcacccccac cgcaaggttt ctcgccgccg cagcgctgct gctgagcctt gccgcctgcg
     1501 ccaccacctc gggccagaaa tgggccgttt caggtggcga ccgctccgac ggggtggtgc
     1561 ggatctccta cgaatacccc gagttccatc agcccacggt cagcgacgaa caggccatga
     1621 aactggcgct ggggcgctgt gaaggctggg gattcgatga cgcgcagccc attgccgggc
     1681 aggtgcgcaa gtgcgccaac atggacggca ccaactgcaa tctctggacg gtcacgcgcg
     1741 aatatcagtg caagacttcc ggcgcgttcg ccagcaactt cgccaagtga ttactgcggg
     1801 ctggccggct tctgcgagaa ggaattgtct aggggaacct ggaaaaaact gccgctgcgt
     1861 atctcgatct tcagcggctc gccggcgtcc ttcaccgact cgtcactcac gtccttaccc
     1921 gtgccgtagt tcacctgcgc catcgggttc ttgttagcgt ccaccagcac gaacaatcgc
     1981 gaccccggca acatctgccg gctcgtcatc gtggtttcga atttcacatg ctcgagcttg
     2041 cccggcttga gcagatttcg cttacccggg tcttcgctgt agctcgcgcg atgcagcgca
     2101 tacgcgaggt ggaacagttt tccatcgggc atcgcttcgt acacggtgac gccgaggtcg
     2161 aaatccttct tgttgatgat cgcaaacagc tcgccggtga acgaaccgct gatgaccgtg
     2221 ggcgtctcga agggctcgct cacgaaaatg ctttcggtga cgtacgacag cggcccctgc
     2281 acgatgggca tgggatacgc atgaaagttg tgaaacttca cgcgatcggc gagatccacc
     2341 tcgtgagtca ccactgagtc cgacggcggc tgccggttcg cgagcgagta cacgccctgg
     2401 tttttctgcg acgagaaata caggcgcatg ggcacgccgc catgcacgga gccgagcgac
     2461 ggcgcgtgtc gccacacatt ggcgcccatc acttcgtagt tgacgatgtt cctgagcagc
     2521 gcgggcttgg gcttgccacg cagtacgtag tccatgaact gcagcttgag ttcctgtgaa
     2581 tcgatctgcg ccaccgggtc gatggtgtat tcgcgcagca cttcgggttt ctgccgcgcg
     2641 ttcgtgccga agtgatcgaa cggaccgatg acgagatagt gctcggggtt gcggcccgtg
     2701 gcggcgtgct ggttgaagta ttgcagcgcc gagatctgcg catcgtcgta gtagccggtg
     2761 atgttgagga tcgggatgtc gatgcgcccg aagtcgcttt tgttcggcac catcgactgc
     2821 cagtacttgt cgtacgccgg atgcatgagc cagcgctgca gccacgggtt gggcgtgccg
     2881 tcgaccgcgt cgatctcgcg atacgggcgg ccgctcgcaa accatttgcg ccccaggttc
     2941 gaccagcgct ggctgtcggc gtatagcgct tcgtcgagcg tcttgttgtt ggtcacgtag
     3001 aacgcccacg ggtagttag
//
LOCUS       DATDEU010000351        10622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1814996, whole genome shotgun sequence.
ACCESSION   DATDEU010000351 DATDEU010000000
VERSION     DATDEU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814996"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..602
                     /locus_tag="VIV63_08335"
     CDS             177..602
                     /locus_tag="VIV63_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6124643.1"
                     /translation="MERLIEYATLHPLMVSAAALSLVVVIAYEARIRATAFAAISAQD
                     LIRLMNQGALVLDIRKPEEFAAGHVNGAKQLPSDQILTAGDNFKRFKEKPVVVYDESG
                     SLSGAAVRQLNLQGFTKAFSLRGGFAGWRAEHLPVTKAA"
     gene            602..862
                     /gene="grxC"
                     /locus_tag="VIV63_08340"
     CDS             602..862
                     /gene="grxC"
                     /locus_tag="VIV63_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY6124644.1"
                     /translation="MSTPAVVMYTTSWCPYCDRARKLLARKGVAIEEIDVESAPEKRA
                     EMRTRSGRTSVPQIFIGDQHVGGSDDLLALEQAGKLDGLLGR"
     gene            902..1384
                     /gene="secB"
                     /locus_tag="VIV63_08345"
     CDS             902..1384
                     /gene="secB"
                     /locus_tag="VIV63_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY6124645.1"
                     /translation="MENQTAPAAAEQNGPMLSIQSVYLKDCSYEAPKGPRIDGQWSPQ
                     ISLDINTSANVVAPDLNEVVLTVTVGAKQGDVTAFLVEVKQAGLFAMRGLNADDTRRA
                     IGTICPNVLFPYARVAVSQLVTQGGFPNFVLPPVDFDQLFARGLQQAAAQAGTQGAAN
                     "
     gene            1389..2453
                     /locus_tag="VIV63_08350"
     CDS             1389..2453
                     /locus_tag="VIV63_08350"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY6124646.1"
                     /translation="MTRRRGDTPARIAVNSASNDISREPVAVLGAGSWGTALAIQFAR
                     AGHPTRLWCRNAADAAQMTAERQNRRYLPDARFPEGIEIHTSLAAAVKGAVAIIVAVP
                     SHSLRSLLTQLKPLLARDARLAWATKGFELDTGKLPHQVAYEVFGDRYPVAVLSGPTF
                     AREVGMGLPTAMTIASPDADFAAQLAKSLHASNFRAYTSTDIVGVEVGGAVKNVLAVG
                     AGLSDGLGFGANTRIALITRGLTEVTRLGVALGAQPETFMGLAGLGDLVLTSTDNQSR
                     NRRFGLALAGGKSVEEALKEIGQVVEGYTAARAVHEVAKRENVDMPIVSGIYRVLYGN
                     EPAKDVVKDLMTRPIRPEVE"
     gene            complement(2481..2939)
                     /locus_tag="VIV63_08355"
     CDS             complement(2481..2939)
                     /locus_tag="VIV63_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY6124647.1"
                     /translation="MFHVVVFEPEIPPNTGNLIRLSANTGCQLHLIKPLGFNLDRKSV
                     RRAGLDYDELATVHVHDNFAAARAAIPGQWFAIETGGSKRHVDARFRPGDALLFGSER
                     RGLPPEILAEIAPSNVLSLPMLAGNRSLNLANAAAVALYEAWRQNDFAGS"
     gene            complement(2960..4330)
                     /locus_tag="VIV63_08360"
     CDS             complement(2960..4330)
                     /locus_tag="VIV63_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY6124648.1"
                     /translation="MRTLTLLVALLAAASASAAEPATTAAPAVPTGATTPKFTESDDI
                     PERTGEDDAFALYEVKLGGEPMTGAEQIARWHDELKAGRARAGSLVGSNLSYLALTPA
                     DCAAAREALVRADELGSDQAAWQIAQLANNPSCGEADVSARELWLKKAVTLDYLAAAQ
                     RLIELYSPAGARSDAVQQYVYARVAGGYWEAVYGKQGNPDSRAGFSVADLQAMETGLA
                     AADRKRAETEATKILTQMLKRHQRFTPVQPVEFARGGQAPKASRGWGYAAQTLDYYHE
                     CAWNLVANCRGAQRLAYVDLVNNDQDFMSCKAELAPKDFVTGKAAKLSRVALVGPKAT
                     RRLVLGDVYDQPDKSAMTVACAVIPQLAANAAAGKCRARLEGTIDAQQFYPPSARNAG
                     IEGDAVVRFFVPPGSDLAADAEIVRSSGYPALDGAAIDTIRSGKFKKECDYGLSSIRI
                     AFKLQD"
     gene            4411..5361
                     /locus_tag="VIV63_08365"
     CDS             4411..5361
                     /locus_tag="VIV63_08365"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY6124649.1"
                     /translation="MAAAAENVPNPLLTDFCGMQLNSPIVLLSGCVGFGEEYTRVEGF
                     SNRDVGAIVLKGTTGSPRLGNRPHRVYETPSGMLNAIGLQNPGVEHVVRKILPTLDFS
                     ETRFFANVSGSTIEEYAEVTRRFDDSPIDAIEINISCPNVKEGGVAFGNYPDMSAKVV
                     EACRKVTNKPIITKLSPNQTDIRENARRCIEAGSNGLAVINTIMGMAIDVKSRRPVIG
                     NIQGGLSGPAIKPIALLKVHQVYEVARKHNVPIIGQGGITTADDALEFMIAGATTVGL
                     GTALFYDPLLCRKINAGVADYLRLNNLRGISELVGSLRTD"
     gene            complement(5402..5560)
                     /locus_tag="VIV63_08370"
     CDS             complement(5402..5560)
                     /locus_tag="VIV63_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124650.1"
                     /translation="MQEIVVQATKYPEIVVQGQRYDYTSDIVLIVVGVIAAAAFIWRY
                     SVARRGKD"
     gene            complement(5900..7333)
                     /gene="ntrC"
                     /locus_tag="VIV63_08375"
     CDS             complement(5900..7333)
                     /gene="ntrC"
                     /locus_tag="VIV63_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY6124651.1"
                     /translation="MHTTPPPTATQPALRVWLVEDDASIRWVLERALRASGMVPRAFD
                     SAEPALAALRADAPDVLLTDIRMPGSSGIDLLREVRVAHPTLPVIVMTAHSDLPSAVS
                     AYEGGAFEYLPKPFDIDQAVELVRRAGQTARKGDDVPAQTPEIPELLGKAPAMQQVFR
                     AIGRLSRSSVAVLITGESGTGKELVARALHEHSPRASKPFVALNTSAIPAELLESELF
                     GHEKGSFTGADTQRRGRFEQANMGTLFLDEIGDMSTPLQTRLLRVLAEGEFYRVGGQT
                     PIKVDVRVIAATHQNLEERVERGIFREDLYHRLNVIRIELPPLRSRTEDVPDLLGHYL
                     RQAANELGTETKSLAAITLERLQAYRWPGNVRELVNLCRRLTALAPGSEIQLQDLPSE
                     MHIVTPAAQTDWAAALTLWAKAQPHDGTVAWLDQALPEFERVLIRVALNRTHGHRQEA
                     ARVLGWGRNTLTRKLKELGMSDDESLP"
     gene            complement(7351..8442)
                     /gene="glnL"
                     /locus_tag="VIV63_08380"
     CDS             complement(7351..8442)
                     /gene="glnL"
                     /locus_tag="VIV63_08380"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEY6124652.1"
                     /translation="MAVEHFLPSAIAHFEPADLIGALTTGIVMLDSDLCVVYANVAAQ
                     DLLAVSVNQARGKPFLSLVLEADSLGALMRRAVETGETLSEREMSVQAGPLGREHRVI
                     DVTMTPLDSEFDRKYLLLEIADATQRQRITRENELISRLDSSRQMVRQLAHEIKNPLG
                     GLRGAAQLLERELHEHLREYTGVIISEADRLTALVDSMIGPARAPQKALINVHELCEH
                     VYRLLRSEARGGVLVERDYDPSLPNAEVDANQIIQALLNVGRNALLAIGERGRIIIRT
                     RIAANVNIGATRHKLAASLQVEDNGPGVPPDIADTIFFPLVTGRANGTGLGLAVSQDL
                     VTRHGGLIEFESKPGRTVFTILLPLPENA"
     gene            complement(8470..9039)
                     /locus_tag="VIV63_08385"
     CDS             complement(8470..9039)
                     /locus_tag="VIV63_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124653.1"
                     /translation="MKRFPLVVLLGSLCAVSTALATTYVRVEKDGTKTYSDRPIPGGQ
                     PIDVQPAQTYSSPPVNYAPSSSSSDDAEPDFRYESCTVTPANDATFTNPESVTIQART
                     NPPLRGSDVISMTVDGSRANGADARSHLMTPVNRGTHTVAVTVTSDDGRVLCTSSSSF
                     HVQRPSLNSPARQGPPPPPRPTPRPRPGN"
     gene            complement(9244..>10622)
                     /gene="glnA"
                     /locus_tag="VIV63_08390"
     CDS             complement(9244..>10622)
                     /gene="glnA"
                     /locus_tag="VIV63_08390"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY6124654.1"
                     /translation="DKEVKYADLRFTDTRGKEQHVTIPANLVDEDLFRDGKMFDGSSI
                     AGWKGINESDMILSLDPATAVIDPFFEQTTVNIRCDVIEPATMQGYERDPRSLGRRAE
                     AYLKSTGIADGALFGPENEFFIFDSVRWNSGLNESSYHIDSVQGAWNSNTVYPEGNKG
                     HRPGLKGGYFPVPPVDAFQDIRGAMCDALEEMGLKVEVHHHEVATGGQCEIGVGPGGL
                     VKKADEVQILKYAIHNVAHSYGKSATFMPKPLVGDNGSGMHVHQSLQKAGVALFAGDK
                     YGGLSETALFYIGGIIKHAKAINAFTNPGTNSYKRLVPGFEAPVMLAYSARNRSASIR
                     IPWVSNPKARRIEVRFPDSAANPYFAFAAMMMAGLDGIQNKIHPGEPADKDLYDLEPE
                     EEKHIPQVCHSLDMALEHLDKDREFLTRGGVFTNDVIDAYIGLKTQEVTRFRMSTHPI
                     EFDMYYSC"
BASE COUNT         1863 a         3345 c         3524 g         1890 t
ORIGIN      
        1 gcggcggacg ccaggcaaaa cgtgaggacg agcgccccgc aaatcgaccg gcaacccctg
       61 aagggaggaa acgtcatggg catattctag agttgcggcc tgctgaaaat ccgcgaaaag
      121 agcgagtgcc gctataatgc gctccctttt tcgcgacccc gggattccaa ggcctcatgg
      181 aacgactgat cgaatacgcg acgcttcacc ctctcatggt gtcggcagcg gcactgtcgc
      241 tggtggtggt cattgcctac gaggcgcgaa ttcgcgccac ggcgttcgcg gcgatctcgg
      301 cgcaggacct gatccggctc atgaaccagg gcgccctcgt cctcgacatc cgcaaacccg
      361 aggaattcgc cgcgggtcac gtgaacggcg ccaagcagct gccgtccgac cagatcctga
      421 ccgccggcga caacttcaag cgcttcaagg aaaagcccgt ggtcgtctac gacgaatcgg
      481 gctcactctc gggcgcggcg gtccgccagc tcaacctcca gggcttcacg aaagcatttt
      541 cgttgcgcgg cggtttcgcc ggctggcgcg cggaacacct gccggtcacc aaggcggcgt
      601 gatgagcacg cccgcggtgg tcatgtacac cacgagctgg tgtccttact gcgatcgcgc
      661 gcgcaagctg ctcgcgcgca agggcgtcgc catcgaggaa atcgatgtcg aatcggcgcc
      721 cgagaaacgc gccgagatgc gcacccgcag cggccgcacc tcggtgccgc agattttcat
      781 cggcgaccag cacgtcggcg gcagcgacga tctgctggcc ctcgagcagg caggcaaact
      841 cgacggcttg ttaggccgct gaccctttca acacagccaa gattcaagca aggaaatagt
      901 catggaaaac cagaccgctc ccgcggcagc agaacagaac ggcccgatgc tctcgatcca
      961 gtccgtgtac ctcaaggact gctcgtacga agcgccgaaa ggtccgcgca tcgacggtca
     1021 gtggagcccg cagatttcgc tcgacatcaa cacgtcggcg aacgtcgtcg cgccggacct
     1081 caacgaagtc gtgctgaccg tgacggtcgg cgccaagcag ggcgatgtca cggccttcct
     1141 cgtcgaagtg aagcaggccg gtctgttcgc gatgcgcggc ctcaacgccg atgacacgcg
     1201 ccgcgcgatc ggcaccatct gcccgaacgt gttgttcccg tacgcgcgcg tcgcggtatc
     1261 gcagctcgtg acgcagggcg gcttcccgaa cttcgtgctg ccgccggtgg acttcgacca
     1321 gttgttcgcc cgcggattgc agcaggccgc tgcccaggct ggcacgcaag gcgcggcaaa
     1381 ctgatcgcgt gacgcgcagg cgtggcgaca cgcctgcgcg catcgccgtg aacagcgcca
     1441 gcaacgacat ctcgcgagaa ccggtcgccg tactcggcgc gggttcctgg ggcactgcgc
     1501 tagccatcca gttcgcgcgc gccggccatc caactcggct gtggtgccgc aacgcggcgg
     1561 atgcggcgca gatgaccgcg gagcggcaga accgccgcta tctacccgac gcgcggtttc
     1621 cggaaggcat cgaaatccac acctcgctcg ccgcggccgt caaaggcgcc gtggcgatca
     1681 ttgttgcggt ccccagccac tcgctgcgct cgttgctcac gcagctcaag ccgctgctgg
     1741 cgcgcgacgc gcgactggcg tgggcaacca agggtttcga gctcgacacc ggcaaactac
     1801 cgcaccaggt ggcatacgag gtgttcggcg accgctaccc ggtggccgtg ctctcggggc
     1861 cgacattcgc gcgcgaagta ggcatgggtc tgccgacggc gatgaccatc gcctcgcccg
     1921 acgccgactt cgccgcgcag ctcgccaaga gcctgcacgc ctcgaatttc cgcgcttaca
     1981 cctccaccga catcgtcggc gtggaagtcg gcggcgcggt gaagaatgtg ctcgcggtgg
     2041 gcgccggcct gtccgacgga ttgggattcg gcgccaacac gcgcattgcg ctcatcacgc
     2101 gcgggctgac ggaagtgacg cggctcggcg tggcgctggg cgcgcagcca gaaacgttca
     2161 tgggtctcgc ggggctcgga gacctggtgc tcacgagcac cgacaatcag tcgcgcaacc
     2221 ggcgtttcgg tctcgctctc gccggcggca agagcgtcga ggaagcgctg aaggaaatcg
     2281 gccaggtggt cgagggttac accgcggcgc gcgccgtgca cgaagtggcg aagcgcgaga
     2341 acgtggacat gcccatcgtc agcggcatct atcgcgtgct gtacggaaac gaacccgcga
     2401 aagacgtggt gaaagatctc atgacgcgcc cgatccgccc cgaagtggaa tgacgcgctg
     2461 atgcccttcg ggcatcccca ctaactaccg gcgaaatcgt tctggcgcca tgcctcgtac
     2521 aaggcaacgg cggcggcgtt ggcgagattg agactgcggt tgcccgcgag catgggcagc
     2581 gacagcacgt tgctaggagc gatctccgcc aggatttccg gcggcaatcc ccgccgctcc
     2641 gacccgaaca gcaacgcatc gcctggcctg aaccgggcgt cgacgtgacg cttcgagccg
     2701 ccggtttcga tcgcgaacca ctgtcccgga atcgccgcgc gcgcggcggc gaagttgtcg
     2761 tgcacgtgca ccgtcgcgag ctcgtcgtag tcgagaccgg cgcggcgcac ggattttcgg
     2821 tcgaggttga acccgagcgg cttgatgagg tgcagctggc aaccggtgtt ggcggacagg
     2881 cggatcaggt tgcccgtgtt aggcggaatt tccggttcga agacgacgac gtgaaacatc
     2941 ccggcgcgcc cggtttcgct cagtcctgca gcttgaaggc gatgcgaatg ctggaaagac
     3001 cgtagtcgca ttccttcttg aatttgccgc tgcgaatcgt gtcgatggcg gcgccatcga
     3061 gcgcgggata accactggag cggacgattt ccgcatccgc ggccaggtcg ctgccgggcg
     3121 gcacgaagaa cctcaccacg gcgtcacctt cgatccccgc gttccgggcc gaaggcggat
     3181 agaactgctg cgcgtcgatg gtgccttcga ggcgcgcacg gcacttgccg gccgcggcat
     3241 tcgcggccag ctgcgggatg acggcgcagg cgacggtcat cgcgctcttg tcgggctgat
     3301 cgtaaacatc gccgagcacg aggcggcgcg tggccttcgg acccacgagc gcgactcggg
     3361 aaagtttggc ggccttgccg gtcacgaaat ctttcggcgc gagctccgcc ttgcaggaca
     3421 tgaaatcctg atcgttgttg acgagatcga cgtaggccag ccgctgcgct ccgcggcaat
     3481 tggcgacgag attccatgcg cactcgtggt aataatcgag cgtctgcgcg gcgtatcccc
     3541 agcctcgcga agccttgggt gcctggccgc cgcgcgcgaa ctccacgggt tgtaccggcg
     3601 tgaaacgctg gtggcgcttc agcatctgcg tgaggatttt cgtggcttcc gtttcggcgc
     3661 gcttgcgatc cgccgcggcc agccccgttt ccatggcctg caggtcggcg acactgaagc
     3721 ccgcgcggct gtcggggttg ccttgtttgc cgtagaccgc ttcccagtaa ccgcccgcga
     3781 cgcgcgcgta gacgtattgc tggacagcat cgctgcgcgc acccgccggc gaatacagtt
     3841 cgatcagccg ctgcgcggcg gccagatagt cgagcgtcac ggccttcttg agccacagct
     3901 ctctcgccga gacgtcggct tcaccgcaac tcgggttgtt agccagctgc gcgatctgcc
     3961 acgcggcctg gtcgctgccg agttcatcgg cccgcacgag cgcctcgcgc gccgcggcgc
     4021 aatcggcggg cgtcaacgcg aggtacgaca ggttgctgcc gaccagtgag ccggcgcgtg
     4081 cgcggccggc cttgagttcg tcatgccagc gcgcgatctg ttcggccccg gtcatcggtt
     4141 caccgccgag cttcacttca tagagcgcga acgcgtcgtc ttcgccggtg cgctcgggaa
     4201 tgtcgtcgga ctcggtgaat ttcggcgtgg tggcgccggt gggcaccgcg ggcgcggcag
     4261 tcgtcgcagg ctcggcggca gaggcggagg cggccgcgag gagggctaca agcagggtga
     4321 gggttcgcat tgggcgcgca tcgtacaacc gctgcacccg tcggcacacg aacaaatgcg
     4381 gggcgtggct tttttcgtac aatgcgcgcc atggccgccg ccgctgaaaa cgtgcccaac
     4441 ccgctgctga ccgacttctg tggcatgcag ctgaactccc cgatcgtgct gctgtccgga
     4501 tgcgtgggtt tcggcgagga atacacgcgc gtcgaaggct tctcgaatcg cgatgtcggc
     4561 gccatcgtgc tcaagggcac caccggcagc ccgagactcg gcaaccggcc gcaccgcgtc
     4621 tacgaaaccc cctcgggcat gctgaacgcg atcgggttgc agaatccggg tgtcgaacac
     4681 gtggtccgga agatcctgcc gacgctcgac ttcagcgaga cgcggttttt cgccaacgtc
     4741 tccgggtcga ccatcgagga atatgccgag gtcacgcgcc ggttcgacga ctcgcccatc
     4801 gacgccatcg agatcaacat ctcgtgtccc aacgtgaagg aaggcggcgt cgccttcggc
     4861 aactatccgg acatgtcggc gaaggtggtg gaagcgtgcc gcaaggtcac taacaagccc
     4921 atcatcacca agctgtcgcc gaaccagacc gacatccggg aaaacgcgcg gcgctgcatc
     4981 gaggcgggct cgaacggcct cgcggtcatc aacacgatca tgggcatggc catcgacgtg
     5041 aagtctcggc ggccggtcat cggcaacata cagggtggtt tgtcaggccc cgcgatcaag
     5101 ccgatcgccc tgctgaaggt tcaccaggtg tacgaggtcg cgcgcaagca caacgtgccg
     5161 atcatcggcc agggcggcat tacgacggcc gacgatgcac tcgagttcat gattgcgggc
     5221 gccacgacgg tgggcctcgg gacagcgctt ttctacgatc cgcttctatg ccgcaagatc
     5281 aatgcgggcg tggcggacta tctcagactc aacaacctgc gcgggatatc cgagctggtt
     5341 ggctcgctgc gcacggactg acgggacgat cagcccgcac gcagcgagtt gtttccgaga
     5401 atcaatcctt gccccggcgc gccactgaat agcgccagat gaaagcggcg gcagcgatga
     5461 ctcccacgac gatcagaacg atgtctgacg tgtagtcgta gcgctgtccc tggaccacga
     5521 tctcggggta cttggtagct tggacgacga tttcttgcat gtttcctctc ttcgccggac
     5581 cgaagaattt ttgtccggaa ccctgatagg gacaaagaat ggaacaagcc cgggacactg
     5641 gaaacaaagg cgttcccgat tcgcgacggc tatcacgcgc cacttcggtt atgtaacccg
     5701 ctaagacaaa acccgggaaa aaagcggtga aaatgcccga cagatagcgc tatcaatctc
     5761 gcatcgacgg cgccgcgaag cgtcgtgtcc ctcggggcgg agtcttgtcc cctgcggtcg
     5821 ccggcgcaat tgcggatgat gcggatatat ctggtttttc ttgagcaggg acaggttggt
     5881 tcaacctgtc cctacccgat cacggcagtg actcgtcgtc cgacatcccc agctccttga
     5941 gcttgcgggt cagcgtgttg cgtccccagc cgagcacgcg cgcggcttcc tggcggtgac
     6001 cgtgcgtgcg attcaacgca acacggatga gtacacgctc gaattccggc agggcctggt
     6061 cgagccatgc cacggtgcca tcgtgcggct gtgccttggc ccagagcgtc agggccgcag
     6121 cccagtcggt ctgcgcggcc ggcgtgacga tgtgcatctc ggacggcagg tcctgcagct
     6181 ggatctcgct gccgggcgcc agcgcagtaa ggcgccggca caggttgacg agttctcgca
     6241 cgttgccggg ccagcgatag gcctggagcc gctcgagcgt gatggcggcc agcgacttcg
     6301 tctcggtgcc gagttcgttg gcggcctggc gcaggtagtg tcccagcagg tcgggcacgt
     6361 cttcggttcg cgatcgcagt ggcggcagct cgatgcggat cacgttgagg cggtggtaaa
     6421 gatcctcgcg gaagatgccg cgttcgacgc gttcttcgag attctggtgc gttgcggcga
     6481 tgacgcgcac gtcgaccttg atgggtgtct gtccaccgac gcggtagaac tcgccttcgg
     6541 cgagcacgcg caacaggcgc gtctgcagcg gcgtcgacat gtcaccgatt tcatcgagga
     6601 acaacgtgcc catgttggcc tgctcgaagc ggccgcgacg ttgcgtgtcg gcgcccgtga
     6661 acgagccttt ctcgtggccg aacaattccg actcgagcag ctcggcggga atggccgagg
     6721 tgttcagggc gacgaagggt ttgctggcgc gcgggctgtg ttcatgcagt gcgcgcgcga
     6781 cgagttcctt gccggtgccg gactcgccgg tgatgagcac ggcgacactc gaacgcgaca
     6841 ggcggccgat ggcgcggaag acctgctgca tcgcaggagc cttgcctaac aactccggaa
     6901 tttccggcgt ttgcgccggc acatcgtcac ccttgcgcgc ggtctggccg gcgcggcgca
     6961 ccagctccac ggcctggtcg atgtcgaaag gcttcggcag gtattcgaac gcgccgccct
     7021 cgtaggcgga caccgcgctc ggcagatccg agtgcgcggt catcacgatg accggcaatg
     7081 tggggtgggc cacgcgcact tcgcgcagca ggtcgatgcc tgagctgcct ggcatgcgga
     7141 tgtcggtcaa cagcacatcg ggcgcatcgg cacgcaaggc cgcgagcgca ggctcggcgg
     7201 aatcgaatgc gcgaggaacc atgccgctcg cgcgcaacgc gcgctcgagg acccaacgga
     7261 ttgaggcatc gtcttccacg agccagaccc gcagggcggg ttgggttgcg gttggcggtg
     7321 gcgtggtatg catataggtg gggtcgacgc tcatgcgttc tccggtaggg gcagcaggat
     7381 ggtgaacacc gttcgcccgg gcttgctttc aaattcgatg aggccgccgt ggcgcgtcac
     7441 gaggtcctgc gagaccgcga ggcccagtcc cgtgccgttg gcgcggccgg tgaccagcgg
     7501 gaaaaagatt gtgtcggcga tgtcgggagg tacgccagga ccgttgtcct cgacctgcag
     7561 actggcggcg agcttgtgcc gcgtcgcgcc gatattcacg ttcgccgcga tgcgcgtgcg
     7621 gatgatgatg cggccgcgtt cgccgatggc gagcagcgca ttgcgtccga cgttgagcaa
     7681 cgcctggatg atctggttgg cgtcgacttc ggcattcggc aggctgggat cgtaatcgcg
     7741 ttccacgagc acgccgccgc gcgcttcgct gcgcaacagg cggtacacgt gctcgcagag
     7801 ttcgtgcacg ttgatgagcg ccttctgcgg cgcgcgcgcc gggccgatca tcgagtcgac
     7861 gagcgcggtc agtcgatccg cctcgctgat gatgacgccg gtgtattcgc gcaggtgttc
     7921 atgcaactcg cgctcgagca gctgcgcggc gccgcgcaac ccgccgagcg gattcttgat
     7981 ctcgtgcgcc agctgccgga ccatctggcg gctgctgtca agacgcgaga tgagttcgtt
     8041 ctcgcgcgtg atgcgctggc gctgcgtggc gtcggcgatc tcgagcaaca gatattttcg
     8101 gtcgaattca ctgtcgagcg gcgtcatggt gacgtcgatg acacgatgtt cacggccgag
     8161 cgggcctgcc tgaaccgaca tttctcgctc ggacaatgtc tcgccggttt ccaccgcgcg
     8221 gcgcatgagt gcgccgagtg agtcggcttc caggaccagc gagagaaacg gtttgcctcg
     8281 ggcctgattc acgctgacgg ccagcagatc ctgcgcggcg acgttggcgt acaccacgca
     8341 caggtccgaa tcgagcatca cgatgcccgt cgtcagcgcg ccgatgagat cggcgggctc
     8401 gaagtgagcg atcgcggaag gaaggaagtg ttcaaccgcc atgcaggccc ctggccatgc
     8461 acaggctggt tagttgccgg gacggggtct cggcgtcggt cggggcggcg gcggtggccc
     8521 ctgacgtgcc ggtgaattca gactgggccg ttgcacgtgg aacgacgaac tcgaggtgca
     8581 cagaacgcgg ccgtcgtcgg acgtcaccgt caccgcgacg gtgtgtgtcc cgcgattcac
     8641 gggagtcatg agatgggatc gggcatccgc tccgttcgcc cgcgaaccgt cgaccgtcat
     8701 cgagatgacg tcgctgccgc gcagcggcgg attcgtgcgc gcctgaatcg tcacggattc
     8761 cggattcgtg aacgtggcgt cgttcgccgg cgtcacggtg cagctctcgt agcggaagtc
     8821 cggctccgcg tcgtccgacg aactcgagct gggcgcgtag ttcacgggcg gcgacgaata
     8881 cgtctgggcc ggctgcacgt cgatgggctg accgccggga atcggccggt cggagtacgt
     8941 cttggtgccg tccttctcga cgcgcacata cgtggtcgcc agcgcagtgg ataccgcgca
     9001 gagcgaaccg agcagaacga ccaggggaaa gcgcttcatg aggctggagc ataaaccctg
     9061 gagatgaacc gggccaaaac gcagcccaca aaactgggcg gcggcgcccg aaacaggccg
     9121 ccgccgccct catatcgatc gaagagtcgg gcaaacgcgg ggccgacaag ctggcgggcg
     9181 caatcgtcgg gacttttccc gaaccttttg ctcccgaggc cagcctccgg ctcacgcgca
     9241 gcctcaacag ctgtagtaca tgtcgaactc gatcggatga gtggacatgc ggaagcgcgt
     9301 gacttcctgt gtcttcaatc cgatgtaagc atcgatcacg tcgttggtga acactccacc
     9361 gcgcgtcagg aactcgcggt ccttgtcgag gtgctcgagc gccatgtcga gcgaatggca
     9421 cacctgcgga atgtgcttct cttcctcggg ctcgagatcg tagagatcct tgtccgccgg
     9481 ttcgcccgga tgaatcttgt tctggatgcc gtcgaggccg gccatcatca tcgcggcgaa
     9541 ggcgaagtac gggttcgcgg ccgaatccgg gaagcgcact tcgatgcggc gcgccttcgg
     9601 gttggacacc cacgggatgc gaatcgacgc cgaacgattg cgcgcggagt acgcgagcat
     9661 cacgggcgct tcgaagccag ggaccaggcg cttgtaactg ttggtgcccg ggttcgtgaa
     9721 cgcgttgatg gccttcgcgt gcttgatgat gccgccgatg tagaacagcg ccgtctcgga
     9781 cagaccgccg tacttgtcac ccgcgaacag cgcgacgccg gctttttgca gcgactggtg
     9841 cacgtgcatg cccgagccgt tgtcgccgac gagaggcttc ggcatgaacg tggccgactt
     9901 gccgtagctg tgcgccacgt tgtggatcgc gtacttgagg atctgcactt cgtcggcctt
     9961 tttcacgagg ccgccgggac ccacaccgat ttcgcactgt ccgccggtcg ccacttcatg
    10021 gtggtgaact tcgaccttga ggcccatttc ttcgagggca tcgcacatcg cgccgcggat
    10081 gtcctggaag gcgtcgaccg ggggaaccgg gaagtagcca cccttcaggc cgggacggtg
    10141 tcccttgttg ccctcggggt acacggtgtt gctgttccac gcgccttgca cggaatcgat
    10201 gtggtacgac gattcgttga ggccgctgtt ccagcgcacg ctatcgaaga tgaagaattc
    10261 gttttcggga ccgaacagcg cgccatcggc tatgccggtg gatttgagat aagcctcggc
    10321 acgacggccg agcgagcgcg gatcgcgctc atagccctgc atcgtggccg gttcgatgac
    10381 gtcgcagcga atgttgaccg tcgtctgttc gaagaaaggg tcgatgaccg cagtggccgg
    10441 atcgagcgac aggatcatgt ccgactcgtt gatgcccttc caacccgcga tggacgagcc
    10501 gtcgaacatc ttgccgtcac gaaacaagtc ttcatcgacg agattcgcgg ggattgttac
    10561 gtgttgttct ttgccgcgcg tgtcggtgaa gcggagatcg gcgtatttga cctctttgtc
    10621 ct
//
LOCUS       DATDEU010000352         4554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1523803, whole genome shotgun sequence.
ACCESSION   DATDEU010000352 DATDEU010000000
VERSION     DATDEU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523803"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..985)
                     /locus_tag="VIV63_08395"
     CDS             complement(182..985)
                     /locus_tag="VIV63_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY6124655.1"
                     /translation="MLEKERHNLILKLVDERSIVSVGDLLDLLGASEATIRRDINALA
                     EKGEVKRIRGGAEAVRPRHQPNLVGMPFAMSQDVSVPQKRAIARAAAELIGPGESIII
                     NGGTTTFALVEFLENSDLDILTNSFPIAAKLFATSRNRITLPGGTIFREQNIVLSPFE
                     NDTIESFWGNKLFVGCYGLNRFGMMEADPLIVQAQTKLLKRSEHVIVMADSSKLRRKS
                     AMIVAGLDRINTIVTDDGAKPEELEDFRSAGIKVIVAKVTEEDELKKLA"
     gene            1124..3259
                     /locus_tag="VIV63_08400"
     CDS             1124..3259
                     /locus_tag="VIV63_08400"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEY6124656.1"
                     /translation="MTVVQMAPQASLEIENRWDDTHAARLSPAELLLYRSNLLGSDKR
                     ITNFGGGNTSAKIKEKDPLTGQEVDVLWVKGSGGDLGTMKLDGFSTLYMEKFLALERF
                     YKAGSDNDAIMVPLYAHSAFNLNPRAASIDTPLHGFVNRAHVDHMHPDALIAIAASAD
                     SKKLTDEIFGGEIGWLPWIRPGYELGLKLRELSQKHPKLKGAVLEAHGLFTWADTSKA
                     CYENTLDIINRATAWLNTNSRGKRPFGAATTQALTIDRRREIAAGIMPTIRGIISAAP
                     EGVTPTLKVGHFDDSETVLDFVGGEKLAGLAALGTSCPDHFLRTKIWPLVLPFDPARD
                     TAEQLAARVKPAIEAYRERYAGYYDRCKRPDSPKMRDANPVVYLVPGVGMITFAADKA
                     TARIAGEFYVNAINVMRGAASVSNYRGLPEQEAFDIEYWLLEEAKLQRMPKPKALAGR
                     VALITGGAGGIGLATARRLMDEGACVVLCDIDREALAGAESELKKKYGNDVVASAWVD
                     VTREDAVIAGFRDSSWKFGGVDICISNAGIASAAPFVDTTLALWQKNMDILATGYFLI
                     ARQAFQLFKSQGIGGSIVFIGSKNALAASPGAAAYCTAKAAELHLARCLALEGAPLGI
                     RVNVVNPDAVLRGSRIWQGEWATQRAAQNKTSVDNLETVYRERSLLKRSVYPEDVAEA
                     VAFFASERSAKSTGNIVNVDAGNAGAFTR"
     gene            3278..>4554
                     /gene="rhaI"
                     /locus_tag="VIV63_08405"
     CDS             3278..>4554
                     /gene="rhaI"
                     /locus_tag="VIV63_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HEY6124657.1"
                     /translation="MQYADPRDASYIASANQSQASALASDYAALGAHLERRGIKIDEI
                     TRAVAGFKVAIPSWGVGTGGTRFGRFPGQGEPRNVFEKLEDCAVIHKLTGATPTVSLH
                     LPWDRTDDYKGLREHGAALGLGFDAMNSNTFQDQVGQPLSYKYGSLTHTDAAVRAQAV
                     AHNIDCIVAGKALGSKALTVWIGDGANFPGQSHLGRAFDRYLESQKQIYAALPEDWRM
                     FIEHKMYEPAFYATVIQDWGSSLMAAQTLGPKAFCLVDLGHHAPNVNIEMIVARLVHA
                     QRLGGFHFNDSKYGDDDLDAGSIEPFRLFLVFNELVEAELAKAPNFKPAHMLDQSHNV
                     TDPVESLMTSAIELQRAYAQALIVDRKALHEYRIGNDALMAAQTLKRGFTTDVSPILA
                     QARVTRGAAIDPVAAYRQSGYRAKCGQMRPGKQ"
BASE COUNT          857 a         1468 c         1408 g          821 t
ORIGIN      
        1 ggcttcgtcg gcgcccttgt ggacggcgtc gaagaaaccg ttgccgagcg acttcgccac
       61 cacgcccacg cgcgtgcgcg cggccttttc cggctcgttc ttgccgcagc cggacagcgc
      121 catgaccagc aggcaaatgc ctgcgcagaa aacctgtttc aactgaaccc ccggctcgcg
      181 ctcaggcgag ctttttcaat tcgtcttctt cggtgacttt cgcgacgatg accttgatgc
      241 ccgcgctgcg gaagtcctcg agctcttcgg gcttggcgcc atcgtcggtg acgatggtgt
      301 tgatgcgatc gagaccggcc acgatcatcg cggacttgcg gcgcaacttt gagctgtcgg
      361 ccatcacgat cacatgctcg ctgcgcttca gcagtttggt ctgcgcctgc acgatgagcg
      421 gatcggcttc catcatgccg aagcggttca gcccataaca tccgacgaac agcttgttgc
      481 cccagaagct ttcgatggtg tcgttctcga aaggactgag cacgatgttc tgctcgcgga
      541 agatcgtgcc gcccggcagc gtgatgcggt tgcggctggt cgcaaacaac ttcgcggcga
      601 tcggaaacga gttggtgaga atgtcgagat ccgagttctc gagaaattcc accagcgcga
      661 acgtcgtcgt gccgccgttg atgatgatgc tttcaccggg accgatgagc tcggccgcag
      721 cgcgcgcgat cgcgcgtttc tggggcacgc tcacgtcctg gctcatggcg aacggcatgc
      781 cgacgagatt cggctgatgg cgcggacgca ccgcttcggc gccgccgcgt atgcgcttga
      841 cctcgccctt ctccgccagc gcgttgatgt cgcggcgaat ggtggcctcc gaggctccca
      901 gcagatcgag cagatctccg acgctcacga tggatcgctc atcgacgagc ttcaggatca
      961 ggttatggcg ctctttttcc aacattgggt caccctcggc ctaggtcgtg atcgattatg
     1021 aacgtgattg attgtatatg acggcttcgt gtgttaaaca gcctcaccat taaatgaggc
     1081 cgtacgtgcg tactttagcg ccggatcgga ccggagacat tggatgactg tggttcagat
     1141 ggccccccag gcgtcactcg aaatcgagaa tcgctgggat gacacccacg cggcccggct
     1201 ctcgccggcc gagctgctgc tttaccgttc gaacctgctg ggcagcgaca agcggatcac
     1261 caacttcggc ggcggcaata cgtccgcgaa gatcaaggag aaggatccgc tcaccggcca
     1321 ggaagtcgac gtgttgtggg tcaagggttc tggcggcgac ctcgggacca tgaagctcga
     1381 cggattctcc actctatata tggagaagtt cctggcgctc gagaggttct acaaggctgg
     1441 ctcggacaac gacgccatca tggtgccgct ttacgcgcac agcgcgttca acctgaatcc
     1501 gcgcgcagcc agcatcgaca cgccgttgca tggcttcgtg aaccgcgcgc atgtggatca
     1561 catgcatccc gacgctctca tcgccatcgc cgcttcggcg gattcgaaaa agctgactga
     1621 cgaaatcttc ggtggcgaga tcggctggct gccctggatc cgcccgggtt acgagttggg
     1681 cctcaagctg cgcgagttgt cgcagaagca cccgaaactc aagggcgccg tgctcgaagc
     1741 gcatgggctg ttcacctggg ccgacacgtc gaaagcctgc tacgaaaaca cgctcgatat
     1801 catcaatcgc gccacggcgt ggttgaacac taattcgcgc ggcaagcgtc ctttcggcgc
     1861 cgccacgacg caggccctga ccatcgaccg gcgccgtgaa atcgccgccg gaatcatgcc
     1921 gacgatccgc ggcatcattt ccgccgctcc cgaaggcgtg acacccaccc tcaaggtcgg
     1981 ccatttcgac gacagcgaaa ccgtgctcga tttcgtgggc ggtgaaaagc tcgcgggtct
     2041 cgcggcgctg ggcacttcct gtcccgatca cttcctgcgc acgaagatct ggccgctggt
     2101 gctgcctttc gatccggcgc gcgacaccgc cgaacagctc gccgcacgcg tgaagccggc
     2161 gatcgaggca taccgcgagc gctacgcggg ctactacgat cgctgcaaac ggcccgactc
     2221 gccgaagatg cgcgacgcga atccggtggt ttacctggtg cccggcgtgg gcatgatcac
     2281 ctttgccgcc gacaaggcca ccgcacgtat cgctggcgag ttttacgtga acgccatcaa
     2341 cgtgatgcgt ggcgcggcca gcgtttccaa ctatcgcggc cttcccgagc aggaagcatt
     2401 cgacatcgaa tactggctgc tcgaagaagc aaagctacag cgaatgccca agccaaaggc
     2461 gctcgccggc cgggtcgcgt tgatcaccgg cggcgccggc ggcatcggcc tcgccactgc
     2521 ccgacggctc atggatgaag gcgcctgcgt ggtgctctgc gatatcgatc gcgaagcgct
     2581 ggccggagcc gagtccgagc tcaagaagaa gtacggtaac gacgtcgtcg cgagcgcctg
     2641 ggtcgacgtc acgcgtgaag acgcggtcat cgccgggttc cgcgacagct cgtggaaatt
     2701 cggcggcgtc gacatctgca tttcgaacgc cggcatcgct tcagcggcgc cgttcgtgga
     2761 tacgactctc gcgctctggc agaagaacat ggacatcctg gccacgggat acttcctgat
     2821 cgcgcgccag gccttccagt tgttcaaatc gcagggcatt ggcggatcga tcgtcttcat
     2881 cggttcgaag aacgccctcg ccgcctcacc gggcgccgcg gcctactgca ctgccaaggc
     2941 tgccgagttg cacctggcgc gctgcctcgc gctcgaaggc gcgccgctgg gcatccgcgt
     3001 gaacgtcgtg aatccggacg ccgtgctgcg tggctcgcgc atctggcagg gtgaatgggc
     3061 gacgcagcgt gcggcgcaga acaagacgag cgtcgacaac ctcgagaccg tctatcgcga
     3121 acgcagcctg ttgaagcgca gcgtttaccc ggaagacgtg gccgaagcgg tggcattctt
     3181 tgcttccgag cgttccgcca agtcgacggg caatatcgtg aacgtcgatg ccggcaatgc
     3241 cggcgcgttc acgcgctaag caatccgaga ggggcctatg cagtacgccg atccgcgcga
     3301 tgcgagttac atcgcgtcgg ccaaccagtc gcaggcgagc gcgctcgcgt ccgactatgc
     3361 ggcgctgggc gcccacctcg aacggcgcgg catcaagatc gacgagatca cgcgcgctgt
     3421 ggcgggcttc aaggtcgcca ttccgtcgtg gggcgtgggc accggcggca cgcgcttcgg
     3481 ccgtttcccc ggacagggcg agccacgcaa tgttttcgag aaactcgaag actgcgccgt
     3541 catccacaag ctgaccggcg ccacgccgac ggtgtccttg cacctgccct gggatcgcac
     3601 cgacgattac aaagggttgc gcgaacacgg ggccgcgctc ggcctcggct tcgacgcgat
     3661 gaactcgaac accttccagg accaggtcgg ccagccgctg tcgtacaagt acgggagcct
     3721 cacgcacacc gacgcggcgg ttcgcgcaca ggccgtggcg cacaacatcg actgcatcgt
     3781 ggccggaaag gcactgggct cgaaggcgct gacggtctgg atcggcgatg gcgccaactt
     3841 ccccggccag tcgcatctcg gccgcgcgtt cgaccgttac ctcgaatcgc agaaacagat
     3901 ttacgccgcg ttgcccgaag actggcgcat gttcatcgaa cacaagatgt acgagccggc
     3961 tttttacgcc acggtgatcc aggactgggg cagttcactc atggcagcgc aaacgctggg
     4021 cccgaaggcg ttctgcctcg tggacctcgg ccaccacgcg ccgaacgtga acatcgagat
     4081 gatcgtcgcg cggctggtgc acgcgcagcg gctcggcggt ttccatttca acgactccaa
     4141 atacggcgac gacgatctcg acgcgggctc catcgagcct ttccggttgt tcctggtgtt
     4201 caacgaactc gtggaagccg aactcgccaa ggcgccgaac ttcaagccgg cgcacatgct
     4261 cgaccagtcg cacaacgtca ccgatccggt cgaaagcctc atgaccagcg ctatcgaact
     4321 gcagcgcgca tacgcgcagg cattgatcgt cgatcgcaag gcgctgcatg aataccgcat
     4381 cggcaacgac gcgctcatgg ccgcgcaaac gctgaagcgg ggcttcacca ccgacgtatc
     4441 gcccatcctg gcgcaggcgc gtgtgaccag gggtgcggcc atcgatccgg tcgccgccta
     4501 tcgccagagc ggctaccgcg ccaagtgcgg acaaatgcgg ccgggcaaac agag
//
LOCUS       DATDEU010000353         3858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_794921, whole genome shotgun sequence.
ACCESSION   DATDEU010000353 DATDEU010000000
VERSION     DATDEU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794921"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..3250)
                     /locus_tag="VIV63_08410"
     CDS             complement(179..3250)
                     /locus_tag="VIV63_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6124658.1"
                     /translation="MNSKLFAGHARRGRAAVYLLTGLALGSGASVLAQDQGAPVAEET
                     ETIIVTGSRIARGVEDASTPLQIIGAEEIKLSGAMSMDKVLNDQPQFVAATNGGATAN
                     TVPAGSAAGAAYVNLRGFGPTRSLTLVNGRRFAIFGPEQVTDLNTIPTALVERTEVVT
                     GGSSAVYGSDAITGVVNFIMKDDFEGVEIGGQYGFDSSTSTPIFSTDLTVGGNFAEGR
                     GNAVVSLNYYKREGFTRHERGDWAELPYGEGCVTAATWSDSLIGTPNGSNAANCAASG
                     GRMGFVFSGSGDIPNGRFVLTDAQRAAVQAQLATAGLTGLGPNGFTFNDAGAPGSQRL
                     VNRPADDFNLTQFNYLQVPQERWMLNAFTHYEFAPKATGYLEFHFSNNRVDQQLTQAN
                     IGGDFLFNTNNPYLDANMRATLVALDAAETGPRTLQQGPIAYTTTPNDGLAILTAGRR
                     LVELPFRQNVDDHNVWRMAVGMRGDLGDASEKFFRNLNYDVYFTFSRSEDSSRQQGAA
                     SRSRYAAALLSSGGNPPVANIFGQNLSEAAVNAIKINATNTTNAEQKVAVATLGGEAF
                     DLPAGAVNFSLGLEWRTAEAEYIPDEYLRSGDVIGFNPGLPTSGDVTAKEIFAEVRVP
                     ILADLPFVQNLTANGGYRSSDYDLDGVGRVETYLYGLDWRVNESVAFRGQFQRAIRAP
                     NIGDLYGGLQLNFQTLTDPCSSRNTANRTASVRALCEATGVPALSVFGAGVQPDNIIP
                     TRSGGNADLQEESSDTTTLGVVLTPTFIDDLVVTIDYFDIKLDGAISQLGGGAQNTLN
                     LCYFTVQDAASDFCQAVHRNPATGGITVPYSLDVLQANIGRLATIGVDLGARYGWDAG
                     FGIEGASRFDISTQWTHTDKFTITPMQAVPANKNRCVGAYGSTCGEPIPEYKGVTRFT
                     WSTGPLGVSLRHRFIDSVTTDRIVLPDTSNVGTVPLAATLTNPKFDAMNYFDLSATFD
                     FGDKAEVYAGVNNVLDEDPPIVAGFGGYGNTFPATYDYAGMTVFMGVNVTF"
BASE COUNT          704 a         1186 c         1228 g          740 t
ORIGIN      
        1 gggcgtcacg ctcgcgctgg acatcattcg caaggaactc gccgtcagcc tcgcactcac
       61 gggcgccaac gacgtacgca aggtcacgcg cgacattctc gtcccctgaa aaaaagaaac
      121 ctccgccgcg cacagctgcg cgacggaggt cgcttgctct cgcgaggggg gcccgcgatc
      181 agaacgtcac attgactccc atgaacaccg tcatgcccgc gtagtcgtaa gtcgcgggga
      241 acgtgttgcc gtagccgcca aagccggcaa cgatcggcgg atcctcgtcg aggacgttgt
      301 tcacgcccgc gtacacctcg gccttgtcgc cgaagtcgaa ggtggccgag agatcgaagt
      361 agttcatggc gtcgaacttc ggattcgtca gcgttgccgc cagcggcacc gtgccgacat
      421 tcgaagtgtc gggcagcacg attcgatcgg tcgtcacact gtcgatgaag cggtgtctca
      481 ggctcacgcc cagcgggccc gtgctccagg tgaagcgcgt gacgcccttg tactccggaa
      541 tcggctcacc gcaggtcgag ccataggctc ccacgcagcg gttcttgtta gccggcaccg
      601 cctgcatcgg cgtgatcgtg aacttgtcgg tgtgagtcca ctgcgtgctg atatcgaagc
      661 ggctcgcacc ttcgatgccg aagcccgcat cccatccata acgcgcaccc aggtcgacac
      721 cgatggtcgc gagccggccg atgttcgcct gcagtacgtc gagcgaatac ggaacggtga
      781 taccgcccgt cgccggattg cgatggacgg cctggcagaa gtcgctggcc gcgtcctgaa
      841 ccgtgaagta gcagaggttc agcgtgttct gcgcgccgcc gcccagctgc gaaatcgcgc
      901 cgtccagctt gatgtcgaag tagtcgatgg tcacgaccag gtcgtcgatg aacgtcggcg
      961 tcagcacgac acccagcgtc gtggtgtccg agctctcttc ctgcagatcc gcattgccgc
     1021 ccgaacgggt gggaatgatg ttgtcaggct gcacgcccgc accgaatacc gacagcgccg
     1081 gcacaccggt cgcttcgcac aacgcgcgca cggatgccgt gcggttcgcg gtgttacggc
     1141 tcgagcaagg atcggtcagc gtctggaagt tcagttgcag accgccgtac aggtcgccga
     1201 tgttcggcgc gcggatggcg cgctggaact ggccgcggaa tgcgaccgac tcgttcacgc
     1261 gccagtcgag gccatacaga taggtttcga cccggccaac gccgtcgagg tcgtaatccg
     1321 aactgcggta gccgccgttg gcggtgaggt tctgcacgaa cggaagatcg gccaggatcg
     1381 gcacgcgaac ttcggcgaag atttctttcg ccgtgacgtc gcccgacgtc ggcaggccag
     1441 gattgaaacc gatcacgtcg cccgagcgca ggtactcatc ggggatgtat tccgcctcgg
     1501 cggtacgcca ctcgagaccc agcgagaagt tgaccgcgcc ggcagggaga tcgaacgcct
     1561 cgccgccgag agtggccacg gccaccttct gctccgcgtt cgtcgtgttc gtggcgttga
     1621 tcttgatggc gttgacggcc gcttcggaaa ggttctgtcc gaagatgttg gccaccggcg
     1681 gatttcctcc gctggacagc aacgcggctg cataacggct gcgtgacgca gcgccctgct
     1741 ggcggctgct gtcctcggaa cgcgagaacg tgaagtacac gtcgtagttc agattgcgga
     1801 agaacttctc cgacgcatct ccgagatcgc ccctcatgcc caccgccatg cgccagacgt
     1861 tgtggtcgtc gacgttctga cggaaaggca gctccaccag gcgtcgcccc gcggtgagaa
     1921 tggccaggcc gtcgttcggc gtggtcgtgt acgcgatggg accctgctgc agcgtccggg
     1981 gacccgtttc ggccgcatcg agcgcaacca gcgtcgcgcg catgttcgcg tcgaggtagg
     2041 ggttgttggt gttgaacagg aaatcgccgc cgatgttcgc ctgcgtgagc tgctgatcga
     2101 cgcggttgtt gctgaaatgg aattccaggt aacccgtggc cttcggcgcg aactcgtagt
     2161 gcgtgaatgc gttgagcatc cagcgctcct gcggcacttg tagatagttg aactgcgtca
     2221 ggttgaaatc atcggccggg cggttcacca gccgctggct gccgggggcg ccggcgtcat
     2281 tgaacgtgaa tccgttggga cccagtcccg taagtcccgc cgtcgcgagc tgcgcctgca
     2341 ccgcggctcg ctgcgcatcc gtgagcacga aacgtccgtt gggaatgtcg ccgctgccgg
     2401 agaagacgaa ccccatcctg ccgcccgacg ccgcgcagtt ggcggcattc gaaccattgg
     2461 gcgtgccgat gaggctgtcg ctccaggtgg ccgcggtgac gcagccttcg ccgtacggca
     2521 gctctgccca gtcgccgcgc tcatggcgcg tgaacccttc acgcttgtag tagttcagcg
     2581 agacgaccgc gttgccgcga ccttcggcga aattgccacc cacggtgagg tcggtgctga
     2641 agatcggcgt gctggtggaa ctgtcgaagc cgtactgacc gccgatttcg acgccttcga
     2701 agtcgtcctt catgatgaag ttgacgacac cggtgatggc gtcggagcca tagaccgcgg
     2761 acgaaccgcc ggtcactact tccgtgcgct cgaccagcgc ggtcggaatc gtgttgaggt
     2821 cggtgacttg ctccgggccg aagatggcga agcggcggcc gttgaccagc gtgaggctgc
     2881 gggtcggtcc gaagccgcga aggttgacgt aggcggcacc ggcagccgaa ccagcgggaa
     2941 cggtgttagc cgtggcgcca ccgttggtcg cggcgacgaa ctgcggctga tcgttgagca
     3001 ccttgtccat gctcattgcg cccgacagtt tgatttcctc ggcgccgatg atctgcagcg
     3061 gtgtcgacgc atcttccacg ccgcgcgcga tgcgcgatcc cgtgacgatg atcgtttcag
     3121 tttcttccgc tactggcgcg ccctggtctt gcgcaagaac gctcgctccg ctccccaacg
     3181 caaggcccgt caaaagatag acggccgcgc gtcctctgcg ggcgtggccc gcaaaaagtt
     3241 tgctgttcat gatttcccct tcgttttgag acttcagacc gggacggggt tctgagtttt
     3301 ccgcgctctg cccccgccgc cgcccgatca tggatgagcg aatttgcttg agcaagaccg
     3361 aaaccgatct tttacgcgcg gggatgaccc tcaaaggggc ctgtttcatt cagggatggg
     3421 catttacgag cgtgcgatcg cccgattgac gggaaggata tgagtgcagg gataggtgga
     3481 gcgccgccag cgaaggggtg ccagcggcgc tccgattgtc gaaaagcgca cagagcgcgg
     3541 acaaggtgcg cttctcggac tcgtcagaac ttcgttttcg aagcccagga aatcggcggg
     3601 ctgtaacagt tgttagcgct cacaataaat cattaccgat cagtaacgat caaatcagtt
     3661 cgctttttat cacagtgccc ggcttgagcg accaaaaatc gtatttatgg gttcagaaat
     3721 cgctgcgcgg gagcctacac agctgatatc gctaccagct agcactgctt taccaatggt
     3781 cgattttttt gcccacgaca aagcgtgagc gaatcaggcg cagctgaacg tcttagcggg
     3841 aagcgagctc agcggcct
//
LOCUS       DATDEU010000354         6478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1378344, whole genome shotgun sequence.
ACCESSION   DATDEU010000354 DATDEU010000000
VERSION     DATDEU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378344"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VIV63_08415"
     CDS             <1..1150
                     /locus_tag="VIV63_08415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6124659.1"
                     /translation="WVFTSFILAYAIFEIPTARWADRTGAGAVLTRIVSWWSIFTLLT
                     AGAFNYVSLLVTRFLFGAGEAGAFPCVARVLSRWVPLRERGTAKGIFFAGAYASAALT
                     TFTVNALLPFMSWRTILLVFGCVGFVWVIAWKRWFRDEPTDHPAANEAERALILADRT
                     EVVAHPAGFEFWGGLLKQRNVLLLCLSYMPNCATFYFCITWLPSYLREHRGFESTELG
                     MLAALPLALAIGTQFLGGFWSDKIVGRFGLRAGRRAPGIVGYGVAALLVVAATLSPDA
                     KTAAVLFALTVATCMLTTASSWSTCVDIGREHSATVSATMNTSGQIAAILMPPIVGYS
                     LKWFGNWNVPFYLLAGLFVMGALCWMFIDPKKPVFEGDETREPVLAAA"
     gene            complement(1151..4177)
                     /locus_tag="VIV63_08420"
     CDS             complement(1151..4177)
                     /locus_tag="VIV63_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY6124660.1"
                     /translation="MKKYLRIGAPLAALWLGVFASVSAQEAPPAPKLKDDMRQPWQRG
                     DWSFERRWLIAGPFPCTLEAECLGAAGGEAALRPSDGQEQKRADGSSVKWHSLKSWTD
                     VFGFDDLQGSRDGAVAYAFTKISRPESGKALLSVGSADGIRVWVNGKLMLSRDGARSL
                     VVDENQVEVDLAAGDNAVLVKLGAANSFTLRVLETGTVLARTAEIGPSFAGFMPAGFS
                     LVTDVNSSRADAPPVKVEVIAPGGAVKHTVTAKRGEQIYVDGKDWPDGPYDVRCSTQD
                     LRGLLKITHLAWYKGDALARARELAAEAAKADASKPEGFTLKMLAEMVDDRLGVKLAD
                     AKANPWPKIHSPLMEFEELMLERAGKVGRVRPDGFVRLAWIDEVDGTPQYARAYLPVD
                     YDPAKKWPMVLQMHGFNPANPVYWRWWSADSRHGLDGEYGDHQGVIYVEPHGRGNVQY
                     MGFADSDILRGIAEAKRLFSVDEDRVYLTGDSMGGWGTWNVSTRHPELFAAIAPVFGG
                     VDYHSTMTEEELAKLTPAARFFREKESSWSMAESLINTPIFVHHGDKDEAVNVDWSRW
                     GVKLLQRWGYDIRYQEYPGKVHEALTTNNGPMNVEWFLKHRRDPNPRKVRIRSAELRN
                     AAAWWARVHQLENPLQFMRVDAEVVDRNVIRLDTENVLDIVLTPGVALIDPAKPVKVV
                     WNGEARDLRVAEGALRLTSKSYKPAKLHKSPALPGSFTPDFFMTPYAVVVGTSSKDPE
                     MVALCKEKAQGFVDAWKEWQKFPPRVFLDTEITDADMARYSLMLIGGPEANRISAKLA
                     ARLPLRLSADAIRIDGKEFKVGDAAVQMLYPNPLNAARYVWMFAGNSTKGMYFAEPNP
                     YRRDDFDYLIMDGRIPAFKQAATRLETNVVSGNFDNNWRYASALSFAGDASIRANGRK
                     LGRPDKNLKIDPKILDSYAGRYQIVEGPVIEMLRKGDKLLVVADGTESELLAENETTF
                     YVTFFNARLFFVKDAAGKVTGFTGYRDSDFEGKKLE"
     gene            complement(4239..6056)
                     /locus_tag="VIV63_08425"
     CDS             complement(4239..6056)
                     /locus_tag="VIV63_08425"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY6124661.1"
                     /translation="MLALVAWMAGVPAAYADERILSFDETITVNADGSLYVREVIRVR
                     AEGDKIRRGIYRDFPTIYSGSNGQRYVVGFAFQGATRDGRPEQSRVENRGNGVRVYLG
                     NPSETVPRGEHTYEIVFRTDRQMGFFAEHDELYWNVTGNGWDFAIDRATARVELPSGI
                     PAADIKLEGYTGAQGSKGQNFQARLDNGVPLFETTRALGAREGLTIVVMFPKGFIMPA
                     VESPASGAAPSDTNYMVDDGRSGWAPAERMFNADLPNNRLPAVLGLVGLALLMAYYYL
                     IWNRVGRDPPGRITIPEYESPKGQSAASMRFLMRMGYDNECFAAAVLGLAVKGYLTIH
                     QDAGILGFGKTFTLIKVPSPQGKPLSADEQQLLASLFSSSDTLVLENKNHLRVSSART
                     KHEKSLKQSFTAGFFSINGGWHLLGIALSLLLAAPAILLPGTTDPWPVWHFASLGGWF
                     TIAMIVAMLVANGVFGILLRAPTVAGQAIMDHIRGFKMYLEVAEGEELKRMKEPPPPL
                     TPQLYESYLPAALALDVEQRWAERFADVLNIKDPNYSPAWYVGSGWNIHNMGSFSRDL
                     GSSLGSAISSASQAPGSKSGGGGGGSSGGGGGGGGGGGW"
     gene            complement(6089..>6478)
                     /locus_tag="VIV63_08430"
     CDS             complement(6089..>6478)
                     /locus_tag="VIV63_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY6124662.1"
                     /translation="VKGYAGYERALLTSLSEMRSQSTATPSPGARGEVEKSVSTGVNK
                     LVALVEAYPDLKASENFRDLQNKLVDTENQIQFARRYYNGAVNLFNNRIQKFPDMLIA
                     NTFGFKPAEFFELEDSKAAAAPGVVLQ"
BASE COUNT         1068 a         2207 c         1986 g         1217 t
ORIGIN      
        1 ctgggtgttc acctcgttca tcctggccta cgcgattttc gagattccca ccgcgcgctg
       61 ggccgaccga accggcgcgg gcgccgtgct cacgcgcatc gtgagctggt ggtcgatctt
      121 cacgctgctg accgcgggcg ccttcaacta cgtgtcgctg ctggtcacgc ggttcctgtt
      181 tggcgccggt gaagcgggcg cgttcccttg cgtggcgcgc gtgttgtcgc gctgggtgcc
      241 gctgcgcgag cgcggcaccg ccaagggcat cttcttcgcg ggcgcctacg cctccgccgc
      301 gctgaccacc ttcaccgtga acgcgctgct gccgttcatg agctggcgca ccatcctgct
      361 cgtgttcggc tgcgtcggct tcgtctgggt catcgcctgg aaacgctggt tccgcgatga
      421 acccaccgac caccccgccg cgaacgaagc cgagcgcgcg ctgatcctcg cggaccgcac
      481 ggaagtcgtc gcgcatcctg cgggcttcga attctggggc gggttgctca agcagcgaaa
      541 cgtgttgctg ctgtgccttt cgtacatgcc gaactgcgcc acgttctatt tctgcatcac
      601 ctggctgcct agctacctgc gcgagcaccg cggcttcgag agcaccgagc tcggcatgct
      661 ggccgcgctg ccgctcgcgc tcgccatcgg gacacagttc ctgggcggct tctggtccga
      721 caagattgtc gggcgcttcg gattgcgtgc cgggcgccgc gcgccgggaa tcgtcggcta
      781 tggggtcgct gcgctgctcg tcgtggccgc gacgctgagt ccggatgcga agacggcggc
      841 ggtgctgttc gcgctcaccg tcgcgacctg catgctgacc accgcgtcgt cctggagcac
      901 ctgcgtcgac atcggccgcg aacactccgc caccgtgagc gcgaccatga acacctcggg
      961 acagatcgcc gcgatcctca tgccacccat cgtcggctac tcgctcaagt ggttcggcaa
     1021 ctggaacgtg ccgttctacc tgctggccgg cctattcgtg atgggcgcgc tgtgctggat
     1081 gttcatcgac ccgaagaagc cggtgttcga aggtgatgag acacgcgagc cggtactcgc
     1141 cgccgcctga tcactcgagc ttcttgccct cgaaatcact gtcgcgatac cccgtgaatc
     1201 ccgtgacctt tcccgccgcg tccttcacga agaacagccg cgcattgaag aaggtgacgt
     1261 agaacgtggt ttcgttctcg gcgagcagtt cgctctccgt cccgtccgcg acgaccaaga
     1321 gcttgtcgcc cttcctgagc atctcgatga caggcccttc gactatctgg tagcggcccg
     1381 cgtagctgtc gagaatcttc ggatcgatct tcagattctt gtcgggacgc ccgagctttc
     1441 tgccattggc acggatgctt gcgtcgcccg cgaaactcag cgcgctcgcg tagcgccagt
     1501 tgttgtcgaa gttgccggag accacattgg tctcgagcct cgtcgccgcc tgtttgaagg
     1561 cggggatacg accatccatg atgaggtagt cgaagtcatc gcggcgatac ggattcggct
     1621 ccgcgaagta catgcccttc gtcgaattgc ccgcgaacat ccacacgtaa cgcgcggcat
     1681 tcaacggatt cggatacagc atctgcacgg cggcatcgcc gaccttgaac tccttgccat
     1741 cgatacggat cgcgtccgcc gacaagcgca gcggcagcct cgcggcgagc ttcgccgata
     1801 tcctgttggc ctccggcccg ccgatgagca tcagcgaata ccgcgccatg tcagcgtcgg
     1861 tgatctcggt atccaggaac acgcgcggcg gaaacttctg ccattccttc cacgcatcca
     1921 cgaagccctg cgccttctcc ttgcacagcg cgaccatctc aggatccttc gacgacgtgc
     1981 ccaccaccac ggcataaggc gtcatgaaaa agtccggcgt gaaggagccg ggcagcgcag
     2041 gagacttgtg cagcttcgcg ggcttgtacg acttgctggt cagacgcagc gcgccctccg
     2101 ccacgcgcag gtcgcgcgcc tcaccgttcc acaccacctt cacgggcttc gcgggatcga
     2161 tcagcgcgac gcccggcgtc agtacgatgt cgagcacgtt ctccgtatcg agccggatca
     2221 cgttgcgatc cacgacctcc gcgtcgaccc gcatgaactg caacggattc tcgagctggt
     2281 gcacgcgcgc ccaccacgcc gcggcgttgc gcagctcggc ggagcggatg cgcaccttgc
     2341 gcggattcgg atcgcgcctg tgcttcagga accactcgac gttcatcggg ccgttgttgg
     2401 tggtcagcgc ctcgtgcacc tttcccggat attcctggta gcgaatgtca tagccccagc
     2461 gctgcagcag cttcacgccc cagcgcgacc aatcgacatt cacggcttcg tccttgtcac
     2521 cgtgatgaac gaagattggc gtgttgatca ggctctcggc catcgaccac gagctctcct
     2581 tctcgcggaa gaaccgcgcg gccggtgtca gtttcgcgag ctcttcctcg gtcatcgtcg
     2641 agtgatagtc cacgccgccg aacaccggcg cgatggcggc aaacaattcc gggtgacgcg
     2701 tggagacgtt ccacgtgccc cagccaccca tggaatctcc cgtgaggtag acgcggtcct
     2761 catccacgct gaacagccgc ttggcttcgg caatgccgcg caggatgtcg ctgtcggcga
     2821 agcccatgta ttgcacgttg ccgcggccgt gcggctccac gtagatcacg ccctggtgat
     2881 cgccatactc gccatcgagc ccgtgacggc tgtcggccga ccaccagcgc cagtacaccg
     2941 gattcgccgg attgaagccg tgcatctgga ggaccatggg ccatttcttc gccgggtcgt
     3001 aatccacggg tagataggcc cgcgcatatt gcggcgtgcc atccacctcg tcgatccacg
     3061 cgaggcgcac gaagccgtcg ggccgcacgc gccccacctt gcccgcgcgc tccagcatga
     3121 gctcttcgaa ttccatgagt ggcgaatgga tcttcggcca cggattcgcc ttcgcgtcgg
     3181 cgagcttcac gcccagccgg tcgtccacca tctcggcgag catcttgagc gtgaagcctt
     3241 ccggcttcga tgcgtccgct ttcgcggctt cggcggcaag ctcgcgcgcc ctcgccagcg
     3301 cgtcgccctt gtaccaggcg agatgcgtga tcttgagcag cccccgaaga tcctgcgtgc
     3361 tgcagcggac gtcgtaaggg ccgtcgggcc agtccttgcc gtcgacgtag atctgttcgc
     3421 cgcgtttcgc ggtcacggtg tgcttcaccg cgccgcccgg cgcaatcact tccactttca
     3481 cgggcggcgc gtccgcccgg ctcgagttca catcggtgac cagcgagaac cccgcgggca
     3541 tgaagccggc gaacgacggg ccgatttccg ccgtacgcgc gagcacggtg ccggtctcga
     3601 gcacccgcag cgtgaaggag ttggccgcac cgagtttcac cagcacggcg ttgtcgccgg
     3661 ccgccagatc cacttccacc tggttttcat cgacgacgag tgaacgcgcc ccgtcgcgcg
     3721 acaacatcaa cttgccgttg acccagacgc gaatgccatc ggcgctacct accgacagca
     3781 gcgccttccc ggattcggga cgcgagatct tcgtgaatgc gtaggccacc gcgccatcgc
     3841 gcgacccttg caggtcgtcg aagccgaaca cgtcggtcca gctcttgagg gaatgccact
     3901 tcacgctcga accgtcggcc cgcttctgct cctgcccgtc cgacgggcgc aacgcagctt
     3961 cgccgcccgc ggcgcccagg cattcggctt ccagcgtgca ggggaatggc ccggcgatga
     4021 gccatcggcg ttcaaagctc cagtcgccac gctgccacgg ctggcgcatg tcgtccttga
     4081 gtttgggtgc gggtggcgct tcctgggcgc tgacgctcgc gaagacaccc agccacagcg
     4141 ccgcaagcgg cgcgccgatc cgcaggtact ttttcaatgg actcccctcg tgtgccagtg
     4201 acttgagacg cgtaatcccg gcaactggtt gcaatgagct accaaccgcc tccgccgccg
     4261 ccaccaccac cgccacccga cgagccgcct cccccacccc ccgacttcga gcccggcgcc
     4321 tgggatgcgg aggaaatcgc gctacccagt gaagaaccca ggtcgcgcga gaagctgccc
     4381 atgttgtgaa tgttccagcc ggagccgacg taccaagccg gagagtagtt cgggtccttg
     4441 atattgagca cgtcggcgaa acgctccgcc cagcgctgct cgacgtccag cgccagcgcg
     4501 gccggcagat aggattcgta gagctgcggc gtgagcggcg gcggcggctc tttcatgcgc
     4561 ttcagctctt caccctcggc cacttccagg tacatcttga atccgcgaat gtgatccatg
     4621 atcgcctgac ccgcgacggt gggcgcgcgc agcaggattc caaacacacc gttggccacg
     4681 agcatggcga cgatcatggc gatcgtgaac cacccgccga gcgacgcgaa atgccacacc
     4741 ggccatggat ccgtcgtgcc cggcagcagt atcgccggcg cggcgagcaa caggctcaac
     4801 gcgatcccga gcaggtgcca gccgccattg atgctgaaga accccgccgt gaaactctgc
     4861 ttgagcgact tctcgtgttt cgtccgtgcg ctgctgacac gcagatggtt cttgttttcg
     4921 agcaccaggg tgtcactgct cgagaaaagg ctggcgagca gttgctgttc atccgccgac
     4981 agcggtttgc cctgcggcga aggcaccttg atgagcgtga aagtcttgcc gaagcccagg
     5041 attcccgcgt cctggtggat ggtcaggtag cccttcacgg cgaggcccag cacggcggcg
     5101 gcgaaacatt cgttgtcgta gcccatgcgc atgagaaagc gcatcgacgc agccgactga
     5161 cccttgggcg attcgtattc cgggatggtg atgcgccccg gcggatcgcg gccgacgcgg
     5221 ttccagatca ggtagtagta agccatgagc agcgcgagac ccacgaggcc gagcacggcc
     5281 ggcagccggt tgttaggcag gtccgcgttg aacatgcgct cggcgggcgc ccagccggac
     5341 cgcccgtcgt cgaccatgta gttcgtgtcc gatggcgcgg cacccgacgc cggcgattcc
     5401 accgcgggca tgatgaagcc cttggggaac atcacgacga tggtgagccc ttcgcgcgcg
     5461 cccagggccc gcgtggtctc gaacagcggc acgccgttgt ccagacgcgc ctggaagttc
     5521 tggcctttcg aaccctgcgc gcccgtataa ccttcgagtt tgatgtcggc cgcgggaatg
     5581 ccggacggca actccacgcg cgccgtggcg cgatcgatgg cgaagtccca cccgttgccg
     5641 gtgacgttcc agtacaactc gtcgtgttcg gcgaagaatc ccatctgccg gtcggtgcgg
     5701 aacacgatct cgtaggtgtg ttctccccgc ggaacggtct ccgacggatt tcccaggtac
     5761 acgcgaacgc cattgccgcg attctcgacg cgcgactgct ccggccggcc gtcgcgcgta
     5821 gcgccctgga acgcgaaccc gacgacgtat cgctgaccgt tgctgccgct gtagatggtc
     5881 ggaaagtcgc gatagatgcc gcggcggatc ttgtcgcctt cggcgcgcac tcgaatgacc
     5941 tcacgcacgt acagcgagcc gtcggcgttc accgtgatgg tttcatcgaa cgacaggatt
     6001 cgttcgtcgg cgtaggcggc cgggacgccc gccatccacg ccaccagggc cagcacgagc
     6061 ccactcttat atatctgccg ccaggcgctc attgcaggac cacgccgggc gcggccgccg
     6121 ccttcgaatc ttcgagctcg aagaactccg cgggcttgaa gccgaaggtg ttggcgatga
     6181 gcatgtccgg gaatttctgg atgcggttgt tgaacaggtt caccgcgccg ttgtaatagc
     6241 gtcgcgcgaa ctggatctgg ttttccgtgt cgaccagctt gttctgcaga tcgcggaaat
     6301 tctcgctggc cttgagatcc ggatacgcct ccacgagcgc caccagcttg ttcacgccgg
     6361 tgctcacgga tttctcgact tcgccgcgcg cgccgggcga tggcgtcgcc gtgctctggc
     6421 tgcgcatctc ggagagactg gtcagcagcg cgcgttcgta tcccgcgtag cctttcac
//
LOCUS       DATDEU010000355         9890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_355897, whole genome shotgun sequence.
ACCESSION   DATDEU010000355 DATDEU010000000
VERSION     DATDEU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355897"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VIV63_08435"
     CDS             complement(<1..821)
                     /locus_tag="VIV63_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124663.1"
                     /translation="MILAGGLVAGCGKSKEDEAAEAATTAESASDETSDESNPFEQAM
                     AERERKREERAAAREAEKAAEAANPKPDPALAAIERLEAKGDEKSKRMAASMRAQHEY
                     MKTISDPKSSQADKKAALDKYVNASRAAGKRDNNVKQPVLDLPPDWAATHREQKNAKE
                     AELLVQVGDIDNLGFGWPFQFDPFAGKSTPTHRFPWAPEADDPPGTDRIMVNSGASAM
                     VVSHDGYTRETYRPDNEPQPLTIEFDPAGVVVKGAVLQLFVDDFQAPMMKTRFSVT"
     gene            997..2112
                     /locus_tag="VIV63_08440"
     CDS             997..2112
                     /locus_tag="VIV63_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6124664.1"
                     /translation="MTYRIVALLAVVIALGACSKKNEAEERRAAEASVAAQMPSEPAT
                     METVDAGGASMPTADAAPDAATGGVAEMTTVAPDFDVNSVPLTSVSLPPFPFFKDPEG
                     LENDLKGMDASKPFDRHFFIAGGKAVAEEGKIALFKYAIEYPPSGRKYSMIEFFRNYD
                     NAIAALGGKKISAVQFTPEVSAAAGGRDAIEKYFNGAPPLVDAEHYSYLIRTADKEYW
                     IHVSAGATVPQIGFVTVLEKQAMQSSLGFLDAAAMKKALDEKGRVALYINFDLDKATL
                     RPDAQPIIEEINKLLASDGSLKLSIEGHTDSSGKADHNRELATARARSVLGALVGLGV
                     DASRLSSKGFGPDKPIADNATDEGRAKNRRVELVKTN"
     gene            2128..2916
                     /locus_tag="VIV63_08445"
     CDS             2128..2916
                     /locus_tag="VIV63_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124665.1"
                     /translation="MNKLIFAAAALLAATTASAGVRIETVTRDMKTKVADGGTQVILV
                     QDGKVRMQIPKSSGGMILKNSIMYVLDDKRKTYMEMDKETMKKTADQAGAAMKQMQEQ
                     MKKMTPEQRAQMEKMMGGNLPPGMMSGKPDVWEAKNTGKSDTSEGRKCQVWVLNKNGK
                     LHEELCVVPFASLPGKEDFEKTFKELTESFQGMADGLPGAGDSIKARNSINGYPVRTR
                     FFEPTGAERAWETVVTKWVEESVPGSSFEIPAGYKKKEMPKMGE"
     gene            complement(2935..3894)
                     /locus_tag="VIV63_08450"
     CDS             complement(2935..3894)
                     /locus_tag="VIV63_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEY6124666.1"
                     /translation="MKGAVAGLIALGLAQSCFAVTLVVGNPKIALYQSVQAAVDALPE
                     SGGTIEIHSGMFREKLVIAKPNVKLVGLGKRPEHVVLVWGDSNKSAGGTGKSTSVWAS
                     GDGFEAHNLTIQNDYHLNNAERSQAVALRLTADRAVLTKVRLLGAQDTLYAASKSPDS
                     PSRQYFRDCYIEGHVDFIFGDAKAFFDRCHIHGIANEIVMITAQSKVKPEQDSGYVFD
                     RCRITADADVKELWLGRAWRPYATVVFLRTRIDAPLQPAGWREWTPGTTDTFKTATYR
                     EYKSTGQGASHATREPSSKQLTKAETAAWSRGRFLSGADGWRP"
     gene            complement(3909..5201)
                     /locus_tag="VIV63_08455"
     CDS             complement(3909..5201)
                     /locus_tag="VIV63_08455"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY6124667.1"
                     /translation="MTTRREFLARSSMSAAAHGAAMTVAARALGADAAASPALLERAR
                     ALLAKAPLIDTHNDLPSMFLDANGGDITPFDLAQRQPKLCADIPSLREGRVGAQYWSI
                     YVDSATQNTQVSLHEAMREFDVALRFIAAHADFQQARTADDIERIHRGGKIACLMGVE
                     GGHMIESSPAVLRVFHALGARYLTLTHWDNVPWADAATDRGEHFGLTGLGKQLVREMN
                     RLGMFADLSHVSADTMRDALKVSRAPVIFSHSNAHALNPHPRNVPDDVLQLLRANGGV
                     IHVNFIRPFIARDGFAWTQKREAKERELHEKQPQLDDEAVKKAVEEWEKATAPAATIA
                     DVADHIDHIRKVAGIDAIGIGADYYDVDEATMVTGLANVTRYPYLFAELLRRGYSDED
                     VMKIAGRNHLRAMRRMEKIASELQRTEAPLITEFKKPA"
     gene            complement(5204..6319)
                     /locus_tag="VIV63_08460"
     CDS             complement(5204..6319)
                     /locus_tag="VIV63_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY6124668.1"
                     /translation="MQAAVRFVLFLLIATAAPASDGPYVFRDGTDAWRAVSVVQEGEQ
                     DKKSVTPLAKGASITVGAVGAVPAFRVKLRAPAQPPPDEIVIPKASPLFVVADTHGEY
                     EILVSMLQKQGIVGQKLEWKFARGHLVLLGDVFDRGPNHTEILWLVYQLEAEARRAGG
                     GVHLVLGNHETMVLLGDLRYLNPKYVQTTAVTGVTSYSQLFDSTSVLGQWLRTLPAVL
                     KINDLLFLHAGISPALNDSQMKLAEINAGIRSVLGGATPADDAQRERFSLLMGSFGPL
                     WYRGYFAEQTNFPTATPADVARTLDTFAVRRILVGHTIVSTVTPLYDGQVIAVQVYPR
                     RNDTGAPNFESILIKRGEFWRAKLDGSRESLSPHPKE"
     gene            6360..7268
                     /locus_tag="VIV63_08465"
     CDS             6360..7268
                     /locus_tag="VIV63_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6124669.1"
                     /translation="MPRLAAALLALFMALPLIAAETPAVTPHAVLVTGASTGIGRKIT
                     EHLAARGYYVYAGARKQADLDALGAIKNVRAVKLDVTRQEDIDAAVAFVTKEGRGLYG
                     LVNNAGIATLGSATETSPEEFHLLMDVNVYGPFRVTRAFAPLILASKGRITTIGSISG
                     ILAPRDLTAYAMSKHAVEAYTDSLAAQMAPLGVLVSVVEPGNFDSDIGKSAAKRMGKE
                     NRMADRSKYKKPDEVAAAVELALFEATPKRRYMVVTDPRESEITIRKQIEQLVQLNEG
                     QPYTYDRDALVKMLDEAMATARPRVQ"
     gene            complement(7315..7896)
                     /locus_tag="VIV63_08470"
     CDS             complement(7315..7896)
                     /locus_tag="VIV63_08470"
                     /inference="COORDINATES: protein motif:HMM:NF040142.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124670.1"
                     /translation="MSQVPQDRQNFRVRRSLWLIAILTVASLYGLEGELFPPHGNSLL
                     YAIALTWLVTIAVGLGTGFIFAGADPALFSLAPWEKDGEIYERAGIRAFRSILLRSGL
                     GWINPNFHVRHSRADWERLLRETHNSEGVHWLAGAVQVVLAIWFLIDGNARYGYVMLL
                     VRIPFDVYPIMLQRWNRGRVYRVLRRRPSAAST"
     gene            complement(8017..>9890)
                     /locus_tag="VIV63_08475"
     CDS             complement(8017..>9890)
                     /locus_tag="VIV63_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124671.1"
                     /translation="EKLLANPGKPIPALWRRRHKQGHWIWLEGVATNLLDDPSVGAIV
                     TNYRDITERLENESRLGEQMRRLALMARITRAIGERQDLRSIFQVVVRSIEEELPVDF
                     CLIGLYEMGENRLTVACVGARTEPVASGLEMIEGTVIPIDENGLSRCVQGQLVYEPSI
                     AESPFPFPKRLASAGMCSLVIAPLLVESQVFGVLVAGRRTANAFSSGECEFVRQAMEH
                     AALAAHQAQLYTALQQAYDDLRTSQQQIMQQERLRALGQMASGIAHDINNAISPISLY
                     TESLLEREKSLSAPGRGQLQVIQRAISDVAQTVARMREFYRPREPQKLLTRVHLNDLV
                     QQVVDLTRVRWNDMPQQRGVTIELSKQIATSLPAVMGVEGEIREALTNLVFNAVDAMP
                     EGGTLTLRTSASEGRVNIDVFDSGVGMDEDAKRRCLEPFFTTKGERGTGLGLAMVYGM
                     VQRHSADIAIESDVGRGTRVQLSFAAAAADAPVQVTTPIAVPAGLHILLVDDDPVLLK
                     SLSDALEADGHTIVTASGGREGIETFREAHEEQKRFSVVVTDLGMPYVDGRQVANAIK
                     SIAPDSPVILLTGWGQRLQAEGDLPAHVDLVLAKPPRLRELREALARCCAKPASSST"
BASE COUNT         1779 a         3168 c         3181 g         1762 t
ORIGIN      
        1 gtgacactga agcgcgtctt catcatcggc gcctggaagt cgtccacgaa caattgcagc
       61 accgcgcctt tcaccaccac acccgcggga tcgaattcga tcgtcagcgg ctgcggctcg
      121 ttgtcaggcc gataggtttc gcgcgtgtag ccgtcgtggc tgacaaccat ggccgacgca
      181 ccggaattga ccatgatgcg gtcggtgccg ggcggatcgt cggcttcggg cgcccacggg
      241 aagcgatggg tcggcgtgga ttttccggcg aaggggtcga actggaacgg ccagccgaag
      301 cccaggttgt cgatgtcgcc cacctgcacc agcagctcgg cttccttggc attcttctgc
      361 tcgcgatgag tggcagccca atcgggcggc aggtcgagca ccggctgttt cacgttgttg
      421 tcgcgcttgc ccgcggcccg cgatgcgttc acgtatttgt cgagcgcggc cttcttgtcg
      481 gcctggctgg acttcgggtc cgagatcgtc ttcatgtact cgtgctgggc gcgcatcgac
      541 gcggccatgc gcttgctctt ttcgtcgccc ttggcttcca gccgttcgat ggcggcgagc
      601 gccgggtcgg gcttgggatt ggcggcttcg gcggcttttt ccgcctctct cgcggccgcg
      661 cgctcctcgc gcttgcgctc gcgttcggcc atggcctgct cgaacggatt cgattcgtcg
      721 ctggtctcgt cggacgccga ttcagcggtc gtggccgctt ccgcggcttc gtcctccttg
      781 ctctttccgc agcccgcgac caatccgccc gccaatatca tggcgagcaa acaaactaat
      841 gaacgagaca tgcatggctc ccaatcctgc acgaccggct ggactctacg ctggaccgtg
      901 catagttcaa agttgcgaag cgacgccctt tgaaaccttt gcatggggct gtgattcacg
      961 gcacagtccc gatgcttttg ggtcagggag agctcgatga cttaccgaat cgttgcgttg
     1021 ctggccgtgg tgatcgcgct gggtgcgtgc tcgaagaaga atgaggccga ggaacgccgc
     1081 gcggccgaag cctcggtggc cgcccagatg cccagcgaac ccgcaacgat ggagacggtg
     1141 gacgccggtg gcgcgagcat gcccaccgcc gatgctgcgc cagatgccgc aacgggcggt
     1201 gtcgcggaga tgaccaccgt cgcgccggac ttcgatgtga attccgtgcc tctgacctcg
     1261 gtgagcctgc cgcccttccc gttcttcaag gaccccgaag gcctggagaa cgatctcaag
     1321 ggaatggacg cgtcgaaacc cttcgaccgc cacttcttca tcgccggcgg caaggccgtc
     1381 gccgaggaag gaaaaatcgc gctgttcaag tacgccatcg aatacccgcc gtcgggccgc
     1441 aagtattcga tgatcgagtt cttccgcaac tacgacaacg ccatcgcggc gctcggcggc
     1501 aagaagatca gcgccgtgca attcacgccg gaagtttccg ccgccgccgg cggtcgcgac
     1561 gccatcgaaa aatatttcaa cggcgcgccg ccgctggtgg atgccgagca ttacagctac
     1621 ctcatccgca ccgcggacaa ggaatactgg atccacgtga gcgcgggcgc cacggtgccg
     1681 cagatcggct tcgtcaccgt gctcgagaaa caggcgatgc agtcgtcgct gggtttcctc
     1741 gacgctgcgg ccatgaagaa ggcgctcgat gaaaagggcc gcgtcgcgct gtacatcaac
     1801 ttcgacctcg acaaggccac gttgcgcccc gacgcgcagc ccatcatcga agagatcaac
     1861 aagctgctgg cgtcggacgg cagcctcaaa ctatcgatcg aaggccacac cgacagctcc
     1921 ggcaaggcgg atcacaaccg cgagctcgcg acggcgcgtg cacgcagcgt gctcggcgcg
     1981 ctggtcgggc tcggggtcga tgcctcacgt ttgagttcga aaggattcgg gcctgacaaa
     2041 cccattgccg acaacgccac cgacgaaggg cgcgcgaaga atcgccgcgt cgaactcgtc
     2101 aaaaccaact agagagcggg acaactcatg aacaaactca tcttcgccgc ggccgcactg
     2161 ctggccgcca ccacggcctc ggccggtgtt cgcatcgaga ccgtcacgcg cgacatgaag
     2221 accaaggtcg ccgatggcgg cacgcaggtc atcctggtcc aggacggcaa ggtccgcatg
     2281 caaataccca agagttcggg cggcatgatc ctcaagaact ccatcatgta cgtgctcgac
     2341 gacaagagga aaacgtacat ggagatggac aaggagacga tgaagaaaac ggccgaccag
     2401 gccggcgctg ccatgaaaca gatgcaggag cagatgaaga agatgacgcc cgagcagcgc
     2461 gcgcagatgg agaagatgat gggcggcaac ctgccccccg gcatgatgtc cggcaagccg
     2521 gatgtctggg aagccaagaa caccggcaag tccgacacct ccgaaggccg caagtgccag
     2581 gtctgggtgt tgaacaagaa cggcaaactg cacgaagaac tgtgcgtggt gccatttgcc
     2641 tcgctgccag gcaaggaaga cttcgagaag actttcaagg aactcacgga gtcgttccag
     2701 ggcatggcgg atggtttgcc gggcgccggc gattccatca aggcccgcaa ttccatcaac
     2761 ggttacccgg tacgaacccg ctttttcgag cccaccggcg cggaacgcgc gtgggaaacc
     2821 gtggtgacca agtgggttga agagtccgtt cccggctcct cgttcgaaat accggccggc
     2881 tacaagaaga aagaaatgcc gaagatgggt gaatgatcct gggtggctag accctcacgg
     2941 acgccagcca tcggcccctg acaggaaacg gccgcgcgac cacgcggccg tttctgcttt
     3001 cgtgagttgt ttgctgctgg gttcacgagt cgcatggctc gcgccctgcc ccgtggactt
     3061 gtattcgcgg taggtcgccg tcttgaaagt gtcggtggtg cctggcgtcc actcgcgcca
     3121 tcccgcgggc tgaagcggcg catcgatgcg cgtgcgcagg aacaccacgg tggcataggg
     3181 acgccacgcg cggcccaacc acagttcctt cacatcggca tcggccgtga tgcggcagcg
     3241 gtcgaacaca tagccgctgt cctgctcggg cttcaccttg ctttgcgcag tgatcatgac
     3301 gatctcgttg gcgatgccat ggatgtggca gcggtcgaag aacgccttgg cgtcgccgaa
     3361 gatgaagtcg acgtggcctt cgatgtagca atcccggaag tattgccggc tgggcgagtc
     3421 cgggctcttg ctggcggcgt acagcgtgtc ctgcgcgcct aacaagcgca ccttcgtcag
     3481 caccgcgcga tcggccgtga ggcgcagcgc cacggcctgc gagcgctccg cgttgttcag
     3541 gtgatagtcg ttctggatgg tgaggttgtg cgcttcgaaa ccgtcgcccg acgcccacac
     3601 cgaagtggac ttaccggtac cgcccgcgga cttgttgctg tcgccccaga ccagcaccac
     3661 gtgctcggga cgtttgccga ggcccacgag cttcacgttc ggtttcgcga tgacgagttt
     3721 ctcgcgaaac atgcccgaat gtatctcgat ggtgccgccg gactccggca gcgcatcgac
     3781 cgcagcctgc acggactggt acagcgcgat cttcggattg cccacgacca aggtcaccgc
     3841 gaagcacgat tgcgccaggc cgagcgcgat gaggcccgca acggcacctt tcattcgtgc
     3901 ccggcgcgtc acgcgggttt cttgaactcg gtgatcagcg gcgcctcggt gcgctgcagt
     3961 tcggacgcga tcttctccat gcggcgcatc gcgcgcaggt gattgcggcc ggcgatcttc
     4021 atcacatctt catcggagta ccctcgccgc agcagctcgg caaacaggta cggataccgc
     4081 gtgacgttgg cgagccccgt gaccatcgtc gcctcgtcga cgtcgtagta atccgcgccg
     4141 atgccgatgg catcgatgcc cgccaccttg cggatgtggt cgatgtgatc ggcgacgtcg
     4201 gcgatggtgg cggccggcgc ggtcgccttc tcccactctt cgacggcttt cttgacggcc
     4261 tcgtcgtcga gctggggttg cttctcgtgc agttcccgct ccttcgcctc gcgcttctgc
     4321 gtccaggcga atccgtcgcg cgcgatgaac gggcgaatga aattcacgtg gatcacgccg
     4381 ccattcgcgc gcagcagttg cagcacgtcg tcgggcacat tgcgcgggtg cggattaagc
     4441 gcgtgggcgt tggagtgcga gaagatcacc ggcgcgcggc taactttcag cgcgtctcgc
     4501 atggtgtcgg cgctcacatg cgaaaggtcc gcgaacattc ccaggcggtt catctcgcgc
     4561 accagttgtt tgccgagacc ggtgagcccg aagtgctcgc cgcgatccgt cgccgcgtcg
     4621 gcccagggca cgttgtccca atgcgtgagc gtgagataac gcgcgccgag cgcgtggaat
     4681 acgcgcagca cggcgggcga actctcgatc atgtgcccgc cctcgacacc catgaggcag
     4741 gcgatcttgc cgccacgatg aatgcgctcg atatcgtcgg cggtgcgggc ctgctggaag
     4801 tccgcgtgcg cggcgatgaa acgcagcgcg acatcgaact cgcgcatcgc ttcgtgcagc
     4861 gacacctgcg tgttctgcgt ggcggagtcc acgtagatcg accagtactg cgcgcccacg
     4921 cggccttcgc gcaacgaggg aatgtccgcg cacaatttgg gttgccgctg ggcgagatcg
     4981 aatggggtga tgtcaccgcc gttcgcgtcg aggaacatcg acggcagatc gttgtgcgtg
     5041 tcgatgagcg gggccttggc gagcagcgcc cgcgcgcgct ccagcaacgc cggactggcc
     5101 gcggcatcgg cgcccagtgc gcgcgcggcg acagtcatcg cggcgccgtg cgccgcggcg
     5161 ctcatcgaag atcgggccag gaattctcga cgggtcgtca tgttcactcc ttgggatgcg
     5221 gactcaggct ttcccgcgag ccgtccagct tcgcgcgcca gaattcgccg cgcttgatga
     5281 ggatgctttc gaagttgggt gcgcctgtgt cattgcgccg cgggtagacc tgcacggcga
     5341 tgacctggcc gtcgtacaac ggcgtcaccg tcgagacgat ggtgtggccg acgaggatcc
     5401 tgcgcaccgc gaatgtgtcg agcgtgcgcg cgacatcggc gggcgtcgcc gtggggaagt
     5461 tggtctgctc ggcgaaatag ccgcgatacc acagcggacc gaagctcccc atgagcagcg
     5521 agaagcgttc gcgttgtgcg tcgtccgcgg gcgtggcgcc gccgagcacc gaacgaatgc
     5581 ccgcgttgat ctcggcgagc ttcatctggc tgtcgttgag cgccggcgaa atgccggcgt
     5641 gcaggaacag caggtcgttg atcttgagca cggccggcag cgttcgcagc cactgcccta
     5701 acaccgacgt ggaatcgaac agctgcgagt acgacgtcac cccggttacc gcggtggtct
     5761 ggacgtactt gggattcagg tatcgcaggt cgccgagcag caccatggtt tcgtgattgc
     5821 ccaacaccag gtgcacgcca ccgcccgccc ggcgcgcttc ggcttcgagt tgatagacga
     5881 gccagaggat ctccgtgtga ttcgggccgc ggtcgaagac atcgccgagc agaaccaggt
     5941 ggccacgcgc gaacttccac tcgagtttct ggccgacgat gccctgcttc tggagcatcg
     6001 acaccaggat ctcgtattcg ccgtgggtgt cggccacgac gaacaacggc gacgctttcg
     6061 gaatcacgat ttcgtcgggc ggcggttgcg ccggtgcgcg aagtttgacg cggaatgcag
     6121 gcacggcgcc gaccgcgccc acggtgatgg acgcaccctt ggcgagcggc gtgacacttt
     6181 tcttgtcctg ttcgccctct tgcacgaccg agacggcccg ccacgcatcc gttccgtcgc
     6241 ggaagacgta gggtccgtcg gatgccggcg ccgcggtcgc gatgagcagg aaaagaacga
     6301 aacggacggc ggcttgcatc ggcgcaggat acattagccg caaacatcag gagttcacga
     6361 tgccacgtct tgcagccgct ttacttgcac tgttcatggc cttgccgctc atcgccgcgg
     6421 aaacacccgc ggtcacgccg cacgccgttc tcgtcaccgg cgccagcacg ggcatcggcc
     6481 gcaagatcac cgaacatctc gcggcacgcg gctattacgt ctacgccggt gcgcgcaagc
     6541 aggccgacct cgacgcgctc ggcgccatca agaatgtgcg agccgtgaaa ctcgacgtca
     6601 cccggcagga agacatcgac gcggccgtcg cgttcgtcac gaaggaagga cgcggccttt
     6661 acggcctcgt gaacaacgcc ggcattgcca cgctgggctc cgccaccgag acctcgcccg
     6721 aggaattcca cctgctcatg gacgtgaacg tgtacggccc gtttcgcgtg acccgcgcgt
     6781 tcgcaccgtt gatcctcgca agcaagggac gcatcaccac catcggctcc atctccggca
     6841 tcctcgcgcc gcgcgatctc accgcatacg ccatgagcaa acacgccgtc gaggcgtaca
     6901 ccgattcgct ggccgcgcag atggcgccgc tcggcgtgct ggtgagcgtg gtcgagcccg
     6961 gcaatttcga ctccgacatc ggcaagagcg cggcgaagcg catgggcaag gaaaatcgca
     7021 tggccgatcg cagcaagtac aagaagcctg acgaagtcgc cgcggccgtc gaactcgcgt
     7081 tgttcgaagc cacgccgaag cggcgttaca tggtggtgac ggatccgcgc gagagcgaga
     7141 tcacgatccg caagcagatc gagcagctcg tgcagctcaa cgagggccag ccgtacacgt
     7201 acgaccgcga tgcgctggtg aagatgctcg atgaggcgat ggcgacggcc aggccgcggg
     7261 tgcagtgacg tagtcaggga gactttgagt gtctctgcgg cagcgtcttc gctgtcaggt
     7321 ggacgccgca cttggtcggc gcctcagcac ccggtacacg cggcctcgat tccagcgctg
     7381 gagcatgatc gggtatacgt cgaaaggaat cctgaccagc agcatcacgt aaccgtacct
     7441 ggcgttgcca tcgatgagaa accagatcgc cagcaccacc tggacggcac ccgccagcca
     7501 gtgcacgccc tctgagttgt gcgtctcccg cagtagccgc tcccaatcgg cgcgactatg
     7561 acgcacatgg aagttcgggt tgatccagcc cagccctgaa cgcagcagga tcgagcgaaa
     7621 ggctcttatg cccgcgcgct cgtatatctc gccgtctttc tcccacggag cgagtgagaa
     7681 cagcgcggga tccgcgccgg cgaagatgaa gccggttcca agtccaacgg cgatcgtcac
     7741 caaccatgtc agggcgatcg cgtagagaag cgaattgccg tgaggaggaa agagctcgcc
     7801 ctccaggcca taaagcgagg cgacggtcag aatcgcgatg agccagagac tacgccggac
     7861 acggaagttc tgcctgtcct gcggcacttg gctcatgtca aacgaacgcg tgcggcatca
     7921 ggccgcgatc tccgcgacgt aacgtgcaaa gtccattagt tagattcacg ggtccatctt
     7981 tcgcagcgcc gaaagcatcc tcggcgacgc tggcggtcag gtgctggacg aagccggctt
     8041 cgcgcagcag cgcgcgagcg cctcacgcag ctcccgcaac cgcggcggct tggcgagcac
     8101 caggtccacg tgcgcgggca gatccccctc ggcctgcaat cgctggcccc agcccgtgag
     8161 caggatcacc ggagaatccg gcgcgatcga cttgatcgcg ttggcgacct ggcgtccgtc
     8221 gacatagggc atgccgaggt cggtcacgac gaccgagaac cgtttctgct cctcgtgcgc
     8281 ctcgcggaag gtctcgatgc cttcgcgtcc gccgcttgcg gtcacgatgg tatggccgtc
     8341 ggcttcgagc gcatcgctca gggacttgag caataccgga tcgtcgtcga ccagcagaat
     8401 gtgcaacccc gcgggcacgg cgatgggcgt cgtgacctga accggcgcgt cggctgcggc
     8461 ggccgcgaag ctcagctgaa cacgggtgcc gcgtcccaca tcgctttcga tggcgatgtc
     8521 cgcgctgtgc cgctgcacca tgccatacac catggcgagc ccgagtccgg tgccgcgctc
     8581 gcccttggtc gtgaagaagg gttcgaggca acgccgcttg gcatcttcgt ccatgcccac
     8641 gccggagtcg aagacatcga tgttcacacg cccttcactg gcgctcgtgc gcagcgtgag
     8701 cgtgccgcct tcgggcatcg catcgaccgc attgaacacg aggttggtga gcgcttcgcg
     8761 gatttcgccc tccactccca tgacggcggg cagcgaggtc gcaatctgct tcgatagttc
     8821 gatggtgacg ccgcgttgct gcggcatgtc gttccagcgc acgcgcgtga gatccacgac
     8881 ctgttgcacg agatcgttga ggtgcacacg cgtgagcaac ttctgcggtt cgcgcggccg
     8941 gtagaactcg cgcatgcgcg cgacggtctg cgcaacgtcg ctgatggcgc gctggatgac
     9001 ctgcagttgc ccgcggcccg gcgcgctgag cgacttctcg cgctcgagca gggattccgt
     9061 gtacagcgaa atcggcgaga tggcgttgtt gatgtcgtgc gcgatgccgc tcgccatctg
     9121 tccgagcgcg cgcagccgtt cctgctgcat gatctgttgc tgcgaggtcc gcaggtcgtc
     9181 gtaggcctgc tgaagcgcgg tgtacaactg cgcctggtgc gccgcgagcg cggcgtgttc
     9241 catggcctgg cgcacgaatt cgcactcgcc gctgctgaag gcgttggcgg tgcgccggcc
     9301 ggccaccagc accccgaaga cctggctctc gacgagcagc ggcgcgatga ccagcgaaca
     9361 cattccggcg ctcgccagcc ttttcggaaa cgggaacggt gactccgcga tggagggttc
     9421 gtacaccagc tgaccctgca cgcaacgcga caggccgttc tcgtcgatgg ggatgacggt
     9481 gccctcgatc atctcgagtc cgctcgccac cggttcggtg cgcgctccga cgcaggcgac
     9541 ggtgaggcgg ttctcgccca tttcgtacag gccgatcagg cagaaatcga ccggcagttc
     9601 ttcttcgata ctgcgcacca ccacctggaa gatgctgcgc aggtcctggc gttcgccgat
     9661 ggctcgcgtg atgcgggcca tgagcgccag tcttcgcatc tgctcgccga gccgtgattc
     9721 gttttccagc cgctcggtga tgtcgcggta gttggtcacg atggcgccga ccgacggatc
     9781 atcgagcagg ttggtggcca cgccctccag ccagatccag tggccttgct tgtgacggcg
     9841 gcgccagagt gcaggaatcg gcttgccggg attcgcgagc agtttttcga
//
LOCUS       DATDEU010000356         5092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1378779, whole genome shotgun sequence.
ACCESSION   DATDEU010000356 DATDEU010000000
VERSION     DATDEU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378779"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VIV63_08480"
     CDS             complement(<1..498)
                     /locus_tag="VIV63_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY6124672.1"
                     /translation="MENPVHRFVASSPRGFGDLLAAELRSFGAADVRERALGVEFSGP
                     LDVAYRACLESRVASRVFFVVSQFTAATDASFYDAARAIDWRKHIDASRTLACDFSGR
                     HPEITHTRFGALRLKDAICDQLRDTTGSRPDVATEQPAVRVHAHANGPNVTISIDLSG
                     EGLHRR"
     gene            complement(498..1493)
                     /locus_tag="VIV63_08485"
     CDS             complement(498..1493)
                     /locus_tag="VIV63_08485"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HEY6124673.1"
                     /translation="MPRIEVPNFQPFAGQHCETVATGTLLAAGGLKLSEPMLFGLGEG
                     LGFIFINLGSLPLPFVGGRSKPFALTEALCGNLGLNCTSNETTSRPKAWAALENPVRA
                     GQPVGLQLDCFHLEYFSQPVHFAGHFVAAHGFDDREVLLVDTAQQGSMQRTSRASLEK
                     ARFAKGPMAAKARTWTIAPPKRLPAIDVATRKAIRANAKQYLSPPFKGASHLGIVKLA
                     ESLPNWLEIAKRPADDLALAALLMERAGTGGSLFRNFYRDFLAEAGEYLASARSQLKK
                     AQALFADSAREWAAIAGAIEHSGRTAQAAPLSDAAQRCRRVAEIEVAAMRLLAAI"
     gene            complement(1496..2929)
                     /locus_tag="VIV63_08490"
     CDS             complement(1496..2929)
                     /locus_tag="VIV63_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY6124674.1"
                     /translation="MDRAHLNISPDSPVAFVDIETTGCAPGMHRIIDVAVIGATGDSV
                     DFEWQSLVNPGVRVPGGITALTGIDNDMLVDAPPFERIAKELRERLEGRVFIAHNVRF
                     DYGFIRREFAATGTAWRSPNLCTVRLSRALYPEMPRHNLDAVMERHGIHIDNRHRAMP
                     DAQVLLAFWRKLRATWSVSELQGALDLASHRATLPAALSPDLPDDLPESPGVYRFFGA
                     DADGRETLLYVGKANILRERVLDHFRGGASDAKSLKLSTQVRRVEWTETAGELGALLL
                     EAREVRETQPVYNRQLRGGGERLTWLFDDGAAAPKLVELDAEVLRTGNAFGTYRAARD
                     ARRALEGLAREHQWCFKLLGLESGEGSCFGLQVGRCKGACVGKEPAPLHMARVKIGLM
                     PLKLKPWPHEGAVVLREGAGERQQWHIIDAWQHLATIDADCEVEQVADLVPAARRNRR
                     EFDIDSYRILTRFMRDSRYPPRPLPRT"
     gene            complement(2911..4416)
                     /locus_tag="VIV63_08495"
     CDS             complement(2911..4416)
                     /locus_tag="VIV63_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY6124675.1"
                     /translation="MPPQISVSAIEWRERAAALKPRSQVFIDGRFVSAANGATFDNIN
                     PATGRSLGVVAAGEQLDIERAVASARAAFRRGVWSQQPPKARKKVLLKLAELMLAHRE
                     ELALLETLDMGKPISDSLAVDIPSAANCIRWYGEAVDKIYDEVAPTGPNALATITREP
                     MGVVGAIVPWNFPLLMASWKIGPVLASGNSLVLKPSEKSPLTALRIAELAVEAGLPEG
                     VFNVVPGFGHTAGKALALSMGVDCIAFTGSTATGKTVMQYAAQSNLKKVSLECGGKSP
                     NIVLADCPDLDKAATAAAYAIFFNQGEMCSAGSRLLVEASIKDAMLEKIAAVGRTMVP
                     GDPLDPMTKLGAIVDETQMKRVLSYIDTGKADGAKVTLGGKRVREDSGGFFVEPTVFD
                     GVLPAMRIAREEIFGPVLATISFKNVDEAIQIANDVEYGLAAAVWTRDINVAHRTARA
                     LRAGTVYVNCYDADDITVPFGGFKQSGIGRDKSLHAFDKYTELKTTWIELT"
     gene            complement(4429..4971)
                     /locus_tag="VIV63_08500"
     CDS             complement(4429..4971)
                     /locus_tag="VIV63_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6124676.1"
                     /translation="MDVGARLKHVRELHGLSQRELARRAGVTNGLISLIEQNRVSPSV
                     GSLKKVLDGVPMSMAEFFTLDVNSAAQVFYSRDELVEIGNEAASLRLVAAQRPGRQLT
                     VLHERYLPDAATGEDMLSHRGEEGGVVVRGKVELTVGGQTRVLEAGDAYYFASATPHR
                     FRNVGSEVCEIISASTPPTF"
BASE COUNT          872 a         1657 c         1679 g          884 t
ORIGIN      
        1 gcggcgatgc aggccctcgc ccgacaggtc gatggagatg gtgacattcg ggccgttggc
       61 atgagcgtgc acgcgcacgg cgggctgctc ggtcgcaacg tcggggcggc tgcccgtcgt
      121 gtcgcgaagc tgatcgcaga tggcgtcctt gagacgaagc gcgccgaaac gcgtatgcgt
      181 gatctccgga tgccggcctg agaaatcgca ggccagcgtg cgcgacgcat cgatgtgttt
      241 gcgccagtcg atggcgcggg ctgcgtcgta gaagctggca tcggtggcag ccgtgaactg
      301 cgagacgacg aagaacacgc ggctcgccac gcgcgattcg aggcaggctc ggtatgcgac
      361 gtccagcggc cccgagaatt ccacgccgag cgcgcgttcg cgcacgtcgg ccgcgccgaa
      421 gctgcgcaat tccgcggcta acaagtctcc gaagccacgc gggctcgaag cgacgaatcg
      481 gtgcaccggg ttttccatca gatagccgcc agcagccgca tggcggccac ttcgatttcc
      541 gccacgcgcc ggcagcgttg ggccgcgtca ctcaatggcg cggcctgcgc cgtgcgcccg
      601 ctgtgctcga ttgcacccgc gatcgccgcc cactcgcgcg ccgagtccgc gaagagcgcc
      661 tgcgccttct tgagctgcga ccgggcgctt gccaggtact cgcctgcttc cgcgaggaaa
      721 tcgcggtaaa aattgcggaa cagcgagccg cccgttccgg cgcgctccat gagcagcgct
      781 gccagcgcga gatcatccgc cggacgcttt gcgatctcga gccagttcgg caacgactcg
      841 gcgagcttca cgattccaag atgggacgcg cctttgaacg gtggcgacag gtattgcttt
      901 gcgtttgcgc gaatcgcctt gcgcgtggcg acgtcgatcg cgggcaagcg cttgggtggc
      961 gcgatcgtcc aggtgcgcgc cttggcggcc atcgggccct tggcgaaccg ggctttttcc
     1021 aggctcgcgc gtgaggttcg ctgcatcgag ccctgctgcg cggtgtcaac gagcagcact
     1081 tcgcgatcgt cgaagccgtg agcggcgacg aaatgacccg cgaagtgcac gggttgcgag
     1141 aagtactcca ggtgaaagca atcgagctgc aacccgacgg gctgtcctgc gcgcaccgga
     1201 ttttcgagcg cggcccacgc cttcggcctg gatgtcgtct cattcgacgt gcagttcaga
     1261 ccgaggttgc cgcacagcgc ctcggtcagg gcaaacggtt tcgagcgacc gcccacgaac
     1321 ggcagcggca gggaacccag gttgatgaag atgaagccaa ggccttcgcc caggccaaac
     1381 aacatgggct cggacaactt caatccgccg gccgcgagca gcgtgccggt tgcgacggtc
     1441 tcgcaatgct ggccggcaaa cggctggaaa ttcggcactt cgattcgcgg catggtcagg
     1501 ttcgcggcag aggccgtggc ggataccgcg agtcgcgcat gaagcgcgtg agaatccggt
     1561 aactgtcgat atcgaattcg cgccggtttc ggcgcgcggc gggaacgaga tcggcgacct
     1621 gctccacttc gcaatccgca tcgatggttg ccaggtgctg ccaggcgtcg atgatgtgcc
     1681 attgctgacg ctcgcccgcg ccttcgcgca gcacgactgc accttcgtgc ggccagggct
     1741 tgagcttgag cggcatcagg ccgatcttga cgcgcgccat gtgcagcggc gctggctcct
     1801 tgccgacgca ggcgcccttg caccggccca cctggaggcc gaagcaggat ccttcgccac
     1861 tttcgaggcc taacaacttg aaacaccact ggtgctcgcg cgccagtccc tcgagcgcgc
     1921 ggcgtgcgtc gcgcgcagcg cgataggtgc caaaggcatt tccggtgcgc aacacttccg
     1981 cgtccagttc cacgagcttt ggcgcagccg cgccatcgtc gaacagccag gtgaggcgct
     2041 cgccgccgcc gcgcaactgg cggttgtaca cgggctgggt ctcgcgcact tcacgcgctt
     2101 cgagcagcag cgcgcccaac tcacccgcgg tctcggtcca ctccacgcgt cggacctgcg
     2161 tggaaagttt caacgacttg gcatcgctcg cgccgccccg gaaatgatcg agcacgcgtt
     2221 cccgcaggat gttcgccttg cccacatata gaagagtctc gcgaccatcc gcgtccgctc
     2281 cgaaaaagcg atagacaccg gggctttcag gcagatcgtc gggcagatcc ggcgacagcg
     2341 cggccggcaa ggtggcgcga tggctcgcga gatcgagcgc gccctgcagt tcggacacgg
     2401 accaggtcgc gcgcagtttg cgccagaacg cgagcagcac ctgtgcatcg ggcatggcgc
     2461 gatgcctgtt gtcgatgtgg atgccgtgcc gctccatgac cgcatccagg ttgtggcgcg
     2521 gcatctccgg atacaacgcg cgcgacagcc gcacggtgca caggttcgga ctgcgccacg
     2581 ccgtgccggt cgcggcgaat tcgcgccgga tgaaaccgta gtcgaaacgc acgttgtgcg
     2641 cgatgaacac ccggccttcg agtctttcgc gaagctcttt cgcgatgcgt tcgaacggcg
     2701 gcgcatcgac cagcatgtcg ttgtcgatgc cggtgagcgc ggtgatgccg ccgggcacac
     2761 gaacaccggg attcaccagg ctctgccact cgaaatcgac ggagtcgccc gtcgcgccaa
     2821 tgacggcgac gtcgatgatg cgatgcatgc ccggcgcgca gcctgtcgtc tcgatgtcga
     2881 cgaaggccac cggagaatct ggcgagatgt tcaggtgagc tcgatccacg tggtcttgag
     2941 ctcggtgtac ttgtcgaagg cgtgcaacga cttgtcgcgg ccgatgccgg attgcttgaa
     3001 gccgccgaag ggcacggtga tgtcgtcggc gtcgtaacag ttcacgtaca cggtgccggc
     3061 gcgcaacgcg cgcgcggtgc gatgcgccac gttgatgtcg cgcgtccaca cggccgcggc
     3121 gagcccgtac tccacgtcgt tggcgatctg gatggcttcg tccacgttct tgaaggagat
     3181 ggtggcgagc accgggccga agatttcttc gcgcgcgatg cgcatggccg gcagcacgcc
     3241 gtcgaacacc gtgggctcca cgaaaaagcc gccgctgtct tcacgcacgc gtttgccgcc
     3301 gagcgtcact tttgcgccgt cggccttgcc cgtgtcgatg tacgacaaca cgcgcttcat
     3361 ctgcgtttcg tcgacgatgg cgccgagctt ggtcatagga tcgagcggat ctccgggcac
     3421 catggtgcgg cccacggcgg cgatcttctc gagcatggcg tccttgatgg acgcttcgac
     3481 cagcagacgt gaacccgccg aacacatctc accctggttg aagaagatgg cataggccgc
     3541 cgcggttgcg gccttgtcga gatcggggca atcggcgagc acaatgttag gcgacttgcc
     3601 accgcactcg agcgacacct tcttgaggtt cgactgcgcg gcgtattgca tcacggtctt
     3661 gccggtggcc gtggagcccg tgaacgcgat gcagtcgacg cccatggaca acgccagcgc
     3721 cttgcccgcc gtgtggccga agccggggac cacgttgaac acaccttcgg gcagtcccgc
     3781 ctccaccgcg agctccgcga tgcgcagcgc cgtcagcgga gatttctccg acggcttgag
     3841 caccagcgaa ttgccgctgg cgagcacggg gcctatcttc caagacgcca tgagcagcgg
     3901 aaaattccac ggcacgatgg cgccgacgac gcccatcggt tcacgcgtga tggtggcgag
     3961 cgcgttcgga ccggtgggcg cgacctcgtc gtagatcttg tcgacggctt cgccatacca
     4021 gcggatgcag ttcgccgccg agggaatgtc gacggccagg ctgtcggaga tgggcttgcc
     4081 catgtccagc gtctctaaca aggccagttc ttcgcggtgg gcgagcatga gctcggccag
     4141 cttcagcagt actttcttgc gcgccttggg cggttgctgc gaccagacgc cgcggcggaa
     4201 ggcggcgcga gcgctcgcga ccgcgcgttc gatgtcgagt tgttcacccg cggcgacgac
     4261 gccgagcgac cgtccggtgg ccggattgat gttgtcgaac gtcgcgccgt tggcggcgga
     4321 gacgaaacgc ccgtcgatga agacctggga gcgaggcttc agggccgcgg cgcgttcgcg
     4381 ccactcaatt gcggaaaccg agatctgtgg gggcatgact taagcgtctc aaaacgtcgg
     4441 cggggtggag gccgagatga tctcacagac ttcgctgccc acattgcgaa agcggtgcgg
     4501 cgtcgcgctc gcgaaatagt aagcgtcgcc ggcctccagg acgcgcgtct gcccgccgac
     4561 ggtcagctcc accttgccgc ggaccaccac gccgccttct tcgccgcgat gcgagagcat
     4621 gtcctcgccg gtggcggcgt ccggcagata acgctcatgc agaacggtca gttgccggcc
     4681 cggacgctgc gcggccacca ggcgtaacga cgccgcttcg ttgccgatct cgacgagttc
     4741 gtcccgggaa tagaacacct gcgcggcgct gttcacatcg agcgtgaaga actccgccat
     4801 ggacatcggc acgccgtcca gcaccttctt gagcgagccc accgacgggc tgacgcggtt
     4861 ctgctcgatc agggagatga ggccgttggt gaccccggca cggcgcgcca gctcgcgctg
     4921 cgacaggccg tgcagttcgc gtacgtgttt gagcctggct ccgacgtcga tgctcatagt
     4981 tcgatgccct tacttttaag gattatgatc ttccgcaact gccggagtcg aacatggccg
     5041 cccaacccga acatacagcc ccctccctcg atgcgtactg gatgcctttc ac
//
LOCUS       DATDEU010000357         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1524915, whole genome shotgun sequence.
ACCESSION   DATDEU010000357 DATDEU010000000
VERSION     DATDEU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524915"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VIV63_08505"
     CDS             complement(<1..331)
                     /locus_tag="VIV63_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124677.1"
                     /translation="MNAMSRWAETITADSRVLLIVNSQTVRRDEIEQATTAIAACTDL
                     SRVSCIAPASLKTWLIQLGLSAANILPARLHGVDLDLAYFLETPRAIRWAAVRRPTVV
                     LGTEPYAP"
     gene            complement(328..>2287)
                     /locus_tag="VIV63_08510"
     CDS             complement(328..>2287)
                     /locus_tag="VIV63_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6124678.1"
                     /translation="FEARGGGLAIWVPATDTELALRQPTQYVEPLRKVSGRTLEFVRF
                     ESGSGITAAQLDHFMRETHLSCDGSIAVDEGAADEGTFEREGAYAGASPYRPQPFDLR
                     TPRARIESNEWFELPMYPATARSIEMFWDARRGPGDAELAPLLDSARLEAFFRLSRIK
                     PEEERHYGRVRVYNWPRPAPALRVTMLDGGAAGADAAISLLRDIKLVIADASGRVSIA
                     GHGELLERSREELVARYAKSPGESLALQQEVHAYLEHIPLESPLRQDYADFARFMPAT
                     LGRALELGSGYGVLAQTLAPRASRYACLELDARMFRGLRHDLGQTGVVADVQQLPFAS
                     GSFDSVVANNVLEHLTDPLQGLREIRRVLAPGGRLFTLIPFDALSSRHELPAHNWKLD
                     RRSLVAAVTAAGFTVSRLTVINLYDRGVPGAFPSCHGYVAMLDAAAGDQPASAKEETL
                     GVPASLTTGHVWLSMRELAGFERWRDRLVVAVAADPSDIEEFQHFGARVAAVQAHGPW
                     PVASGSADLVYSFLTLTPAELPAVIAEIKRVLAPNGQVVAVFRNRQGLRYLSRICSYV
                     GSACDIGRLFGLDAAVALVDGAGTDADYVTEQEVRKAFSEFGPVEVSIQNLVPEDLAT
                     PLAANYPDEFWRWLSAVAGRFVLVRARQ"
BASE COUNT          462 a          740 c          717 g          368 t
ORIGIN      
        1 tcggcgcgta cggttccgtt ccgagcacaa ccgtcggccg gcgcacggcg gcccaccgaa
       61 tcgcccgcgg tgtctccaga aagtacgcca ggtcgagatc gacaccatgc agtcgagcgg
      121 gcaggatatt cgcggccgac aggcctagtt gaatgagcca ggtcttgagt gatgcaggcg
      181 cgatgcacga tactcgcgag agatcggtgc acgccgcaat cgcggtggtc gcctgctcga
      241 tttcgtcccg gcggacagtc tgggagttca caatcaacag cacgcgacta tcggcagtaa
      301 tcgtttccgc ccatcgggac atcgcgttca ttgccgcgcc ctcacgagca caaagcggcc
      361 ggccactgcc gacagccaac gccagaactc gtccgggtag ttggcggcca ggggcgtggc
      421 gagatcctcg gggacaagat tctgaatcga cacctcgacc gggccaaact cggagaaagc
      481 cttgcgcacc tcctgttcgg tgacgtagtc cgcatcggtt ccggcaccgt cgaccagcgc
      541 gacggcggca tccagcccaa acaggcgacc aatatcgcag gctgaaccga catacgagca
      601 gatgcgtgac aaatatcgaa gtccctgacg attccgaaac acggccacca cctgaccatt
      661 cggcgccaat acgcgtttga tctcggcgat gacggccggc agctcggccg gtgtgagtgt
      721 cagaaacgag tagaccaagt ccgcgctgcc gctagcgacg ggccagggtc cgtgcgcctg
      781 cactgccgcg actcgagcgc cgaagtgttg gaactcctcg atgtcacttg ggtccgcggc
      841 cacggcgacc accagtcgat cacgccagcg ctcgaagccg gccagctcgc gcattgacag
      901 ccacacgtgc ccggtggtca gcgacgccgg gacgccgagc gtttcctctt ttgcggaagc
      961 gggctggtcg cctgctgccg catcgagcat ggcgacgtag ccgtgacagc ttgggaatgc
     1021 cccggggacc ccgcgatcat agagattgat cacagtcagc cgcgagacag tgaaacctgc
     1081 ggcagtcacg gcagcgacaa gcgaacgccg atcgagtttc cagttgtgcg caggtaactc
     1141 gtgacggcta ctgagagcgt cgaatgggat cagcgtaaag agacgcccac ccggcgcgag
     1201 cacgcgccga atctctcgca atccttgaag cggatcggtg agatgctcga gcacattgtt
     1261 ggcaacgacc gagtcaaacg aaccggaggc aaacggcagc tgctggacat cggcaacgac
     1321 cccggtctgc ccgagatcgt ggcgcagacc acgaaacatc cgcgcatcga gctcgagaca
     1381 cgcatagcga ctcgctcgag gagccagcgt ttgcgcgagg accccgtagc cgctgccaag
     1441 ctccagcgcc ctgccaagcg tcgcgggcat gaagcgggcg aaatccgcgt aatcctgtcg
     1501 cagtggagac tcgagcggga tgtgttcaag atatgcgtgc acttcctgct gcagcgccag
     1561 tgactcaccc ggagacttcg catagcgggc gacgagctct tcacgcgacc gctcaaggag
     1621 ctcgccgtgg cctgcaatag atacgcgccc ggatgcgtcc gcgatcacga gcttgatgtc
     1681 gcgaagcagg ctgatggctg catcagcgcc ggcggcaccg ccgtcgagca tcgtgacccg
     1741 cagcgccggt gccgggcgtg gccagttgta gacgcgtacc cggccgtaat gacgctcttc
     1801 ctccggtttg atgcggctta aacgaaagaa ggcttccagt cgcgcgctgt cgagcaaggg
     1861 tgcgagttcg gcgtcacctg gaccgcgccg ggcgtcccaa aacatctcga tggaacgcgc
     1921 cgtggccgga tacatcggca actcgaacca ctcgttggat tcgatacggg cccgcggcgt
     1981 ccgcaagtcg aacggctggg gacggtaggg cgatgcgccg gcgtacgcgc cctcgcgttc
     2041 gaacgtccct tcatcggcag cgccttcatc aaccgcgatc gatccatcgc acgacaggtg
     2101 tgtttcgcgc atgaagtgat cgagctgtgc ggccgtgatc ccactgcctg actcgaatcg
     2161 aacgaactcc aaggtacgac ccgacacctt gcgcagcggc tcaacgtact gagtcggttg
     2221 gcgcagcgcc aattcggtat cggtggccgg cacccatatc gccaagccac cgccgcgagc
     2281 ttcgaac
//
LOCUS       DATDEU010000358         2697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_65806, whole genome shotgun sequence.
ACCESSION   DATDEU010000358 DATDEU010000000
VERSION     DATDEU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65806"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VIV63_08515"
     CDS             <1..1100
                     /locus_tag="VIV63_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY6124679.1"
                     /translation="AAFFPSIELTGFYGNASNDLSALFQSGQTAWSFTSQVNLPIFSG
                     GRNLAALEDYFRLEETRTAAQLVLVSEVANAWLTLIADRQLLKLAQETHDSQRKSYDL
                     TKLRFDSGVSSEIDLHRSETAWREAEIDIAQQTRRVAQDRNALALLVGEPLPAEIAAG
                     ERAFEAQTFARTLPAGLPAELLVRRPDVRAAEHALKAANADIGAARAAFFPSIELTGF
                     YGNASNDLSALFQSGQTAWSFTPQVNLPIFSGGRNLAALDLANVRKRIEIAQYEQSIQ
                     IAFREVADALVTRATLDDQLRAQEALTHASDLSYKLADTRYRGGVDSYLSALIAQRDW
                     YTAQRQLIVTRLAGAVNLVQLYTTLGGGWKE"
     gene            complement(1119..>2697)
                     /gene="ggt"
                     /locus_tag="VIV63_08520"
     CDS             complement(1119..>2697)
                     /gene="ggt"
                     /locus_tag="VIV63_08520"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6124680.1"
                     /translation="DVLRNGGNAIDAAVAVGYALAVVYPAAGNLGGGGFMTLQLADGR
                     KTFIDFRETAPLAATATMYLDASGNVIPDLSTRGYLAVAVPGTVSGLEYARAKYGTMP
                     RTKLIAPSIVLAERGFVLSQGDVDLLHEATDDFRKDAASAAIFLPDNQPLMAGSTFRQ
                     RDLARTLRNISQRGTDGFYKGETAAALIAASRAGKGILTQADFDAYRTRELAPVECDY
                     RGFHIVAAPPPSSGGIVLCEMLNILEGYPLRDYGYASARAVHVTIEAMRHAYVDRNHL
                     LGDPAFVDNPTAKLLDKSYAAKLRSRIDPQRAGSSAQLKPGIAAREGTHTTHYSIADR
                     FGNAVAMTYTLNDWFGARVTAAGTGVLLNNEMDDFTAKAGVANMYGLVQSDTNRIEPG
                     KRPLSSMTPTIVSRDGKPWMIVGTPGGSRIITAVMQTLLNVIDFDMDLQEAVDAPRIH
                     QQWLPEETYVEARAVSPDTRALLVAMGHKLIAPQPENHVAAILIGAPALNAKPVAGMV
                     FYGAIDPRRNTGLALGY"
BASE COUNT          442 a          858 c          932 g          465 t
ORIGIN      
        1 gggccgcgtt cttcccgagc atcgagctca ccgggtttta tggcaacgcg agcaacgatc
       61 tgtcggcgct gttccagtcc ggtcagacgg cctggagttt cacgtcgcag gtgaatctgc
      121 cgatcttctc cggcggtcgg aatctggcgg cgctcgaaga ctatttccga ctcgaggaga
      181 cgcgaaccgc cgcgcaattg gtgctggtgt ccgaggtcgc gaacgcgtgg ctgacgctga
      241 tcgcggatcg gcagttgctc aagctcgcac aggaaacgca tgacagccag cgcaagtcct
      301 acgacctgac caagctgcgc ttcgactcgg gcgtgtcgag cgagatcgac ctgcaccgct
      361 ccgagactgc gtggcgcgag gccgaaatcg acatcgccca gcagacccgc cgcgtcgccc
      421 aggatcgcaa tgcgctggcg ctgctcgtgg gtgagccgct gcccgcggag atcgccgccg
      481 gtgaacgcgc attcgaagcg cagacgttcg ccaggacgct gccggccggg ttgccggcgg
      541 aactgctggt acgccggccc gacgtgcgtg ccgccgagca cgcgctcaag gcggccaatg
      601 ccgacatcgg cgcggcacgg gccgcgttct tcccgagcat cgagctcacc gggttttatg
      661 gcaacgcgag caacgatctg tcggcgctgt tccagtccgg tcagacggcc tggagcttca
      721 cgccgcaggt gaatctgccg atcttctccg gcggtcggaa tctggcggcg ctcgatctcg
      781 ccaacgtgcg caagcgcatc gagatcgcgc aatacgagca gtcgatccag atcgcgtttc
      841 gcgaagtggc cgatgccctc gtcacgcgcg ccacgctcga cgaccagctg cgtgcgcagg
      901 aagcgctgac gcacgccagc gacctgagct acaagctcgc ggatacgcgc taccgcggcg
      961 gtgtggacag ctacctgagc gcgctgatcg cgcagcgcga ttggtacacg gcgcagcggc
     1021 agctcatcgt gacgcggctg gcgggcgcgg tgaacctcgt gcagctctac acgacattgg
     1081 gcggcggctg gaaggaatag ccgcgcgcca ggcggcgcct aataacccag cgcgagcccg
     1141 gtgttgcgtc gcgggtcgat ggcgccgtag aaaaccatgc cggctacggg cttcgcgttg
     1201 agcgcgggcg cgccgatcag gatggccgcc acgtgattct cgggctgcgg cgcgatgagt
     1261 ttgtgaccca tggcgaccaa taacgcacgc gtgtccggac tcaccgcgcg ggcctcgacg
     1321 taggtctctt caggcaacca ttgctggtgg atgcgcggcg cgtcgaccgc ttcctgcaga
     1381 tccatgtcga agtcgatcac gttgagcagg gtctgcatca ccgcggtgat gatccggctg
     1441 ccgcccggcg tgccgacgat catccagggc ttgccgtcgc gcgagacgat ggtcggcgtc
     1501 atcgagctca gcggccgctt gcccggctcg atccggttgg tgtcgctctg cacgagacca
     1561 tacatgttgg cgacaccggc cttggccgtg aagtcatcca tttcgttgtt gagcagtacg
     1621 ccggtgccgg ccgcggtcac gcgcgcgcca aaccagtcgt tgagcgtgta agtcatggcg
     1681 accgcgttgc cgaagcgatc ggcgatcgag tagtgagtgg tgtgagtgcc ttcgcgggcc
     1741 gcgataccgg gtttgagctg cgccgagcta ccggcgcgct gcggatcgat ccgggaacgc
     1801 agcttcgcgg cgtagctctt gtcgagcagc tttgcggtgg gattgtcgac gaacgcggga
     1861 tcgccgagca ggtgattgcg atcgacatac gcgtggcgca tcgcttcgat cgtgacatgc
     1921 acggcgcgcg cggacgcata gccgtagtcg cgcagcggat aaccttccag gatgttgagc
     1981 atctcgcaca acacgatgcc gccggaactc ggcggcggcg cggcgacgat gtgaaatccg
     2041 cggtaatcgc attcgaccgg cgcgagttcg cgcgtccggt acgcgtcgaa atccgcctgc
     2101 gtgaggattc ccttgcccgc gcggctggcg gcgatgagcg cggcggcggt ttcgcccttg
     2161 tagaaaccat cggtgccgcg ctggctgatg ttgcgcaggg ttcgcgcgag gtcgcgctgg
     2221 cgaaacgttg agcccgccat cagaggctgg ttgtcaggca ggaatatcgc ggccgacgcg
     2281 gcatctttgc ggaagtcgtc ggtggcttcg tgcagcaggt cgacatcgcc ctgggagagc
     2341 acgaacccgc gttccgccag cacgatggat ggtgcgatca attttgtgcg cggcatcgtg
     2401 ccgtacttcg cgcgcgcgta ttcgagtccg gacacggtgc cgggaaccgc gaccgcgaga
     2461 tacccgcgcg tgctcaggtc cgggatcacg ttgccgctgg catcgaggta catcgtcgcc
     2521 gtggcggcga gcggcgcggt ctcgcgaaag tcgatgaagg tcttgcggcc atcggcgagc
     2581 tgcagcgtca tgaagccacc gccgccgaga ttgccggccg ccggatacac cacggcgagc
     2641 gcgtagccca ccgcgacggc cgcgtcgatt gcattgccgc cgttgcgcag cacgtcg
//
LOCUS       DATDEU010000359         3513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_505214, whole genome shotgun sequence.
ACCESSION   DATDEU010000359 DATDEU010000000
VERSION     DATDEU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505214"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2258
                     /locus_tag="VIV63_08525"
     CDS             <1..2258
                     /locus_tag="VIV63_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6124681.1"
                     /translation="NFTNSDPFGTSGGNLRIRGFDGSRVSVTFDGVPLNDSGNYALFT
                     NQMLDSELIDRVDVNLGTTDVDSPTASATGGTVAYRTKRPEDEFSGTAVLSGGDFDYK
                     RAFLRIDTGEFGPWGTKAFFAGSYQNYDKFKGPGELEKTQFNAMIRQDFENENFISVG
                     FHYNENRNAFYRTTSAANYRLYGRDYDNLDVCTRDLPTAGVVDNENASPVATIPGVLL
                     NTDNPANTSSCTNYYGVRINPSNTGNVRVQSLWNLSENLRLTFDPSYQYTLANGGGTT
                     ALAETASPTSPDVRVRGNSTALGVDLNGDGDLLDTVRFYTPNTTNTNRYGATASLIWD
                     LTDDHLLRFAFTYDHASHRQTAQWGPMSFEDGEPQDVFAGREGDRVYAADGDIIRGRD
                     RYSVAELTQYALEYRGQFLEDKFTTTVGLRAPFFKRDLNQYCYTPNGGSGSSGTIQAA
                     GGALCTSRAPLTTLPNGNVTFAPAPMGGTPVAFIPPYSETVEFDDLLPNAGLSFRPWD
                     RHQFYLSYAEGLSAPRTDNLYSVVRNPGQTEVTRPTPESEMTKSIDLGWRLNHPTNIA
                     SLALYHIDYTNRIVSTFNEALGYSEDRNVGDVKIQGFDAQAGHRFGESLTLTVSASYN
                     DSELEGSLNPLLDGKQLVETPEWTYSARMDLDVTEDFYVGLQAKKVGDRFATDNNDEI
                     APGYTVVDIDLNYGFKLWGMERAELQLNVTNLLDEEYFGNISSGTGGTSVGFYSIGAP
                     RTVVASIRFDF"
     gene            2272..3507
                     /locus_tag="VIV63_08530"
     CDS             2272..3507
                     /locus_tag="VIV63_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY6124682.1"
                     /translation="MRSVLAFLLLAAVGILPAAAADRPAGLVRIATFNCSLNRAALGD
                     LRRDLATPDNVQARAVAEIIQRVRPDILLLQEFDYDAEGASLRDFQANYLGRPQNGAE
                     AIQFAHSFFTESNTGQPSGFDLNNDGEVGGGQDALGFGEFPGQYAMALLSRFPIDHAQ
                     ARTFRKFVWRDMPGAMLPADWYSAQELAVLPLSSKSHWDVPVRIGKHTLHLLASHPTP
                     PAFDGPEDRNGKRNHDEIRFWNDYLTKANAGYIRDDAGKRGGFRGKAFLIMGDQNSDP
                     VDGASIHGGIGALLAHPRVDSSFVPQSAGAAEASAVQGGANAEQRGDPHTDTADFNDR
                     VAGNLRVDYLLPSKGLRVCDGGVYWPAQAEPAATLVWGDRPAPSSDHRLVWLDVSVSG
                     ARCPPGSDPTTSGSSHPGR"
BASE COUNT          658 a         1175 c         1077 g          603 t
ORIGIN      
        1 tcaacttcac caacagcgat ccgttcggca cttcgggcgg caacctgcgc attcgcggct
       61 tcgacggctc gcgcgtatcg gtgacgttcg acggtgtgcc gctcaacgat tccggcaact
      121 acgcgctgtt caccaaccag atgctcgact ccgagctgat cgaccgcgtc gacgtgaacc
      181 tgggcacgac cgatgtcgac agccccacgg cgtccgccac cggcggcacg gtggcatatc
      241 gcacgaagcg acccgaagac gaattcagcg gcacggccgt gctgtcgggc ggcgacttcg
      301 actacaagcg cgcgttcttg cgcatcgata ccggtgaatt cggaccctgg ggcacgaagg
      361 cattcttcgc gggctcctac cagaactacg acaagttcaa gggcccgggc gaactcgaga
      421 agacgcaatt caacgcgatg atccgccagg acttcgagaa cgaaaacttc atttcggtcg
      481 gattccacta caacgaaaat cgcaatgcgt tctatcgcac caccagcgcg gccaactacc
      541 gtctgtacgg acgcgactac gacaatctcg atgtgtgtac acgtgacctg ccgaccgctg
      601 gcgtggtcga caacgaaaac gcctcaccgg tcgccaccat cccgggcgtg ctgctgaaca
      661 ccgacaatcc tgccaacacg tccagctgca ccaactacta tggcgtgcgc atcaatccgt
      721 cgaacaccgg caacgtccgt gtgcagtcac tgtggaacct gagtgagaac ctgcgcctca
      781 cgttcgatcc gtcgtatcag tacacgctgg cgaacggcgg cggcacgact gcgctcgccg
      841 aaaccgcttc gcccacctcg cccgatgtac gcgtccgtgg caactccacg gcgctgggcg
      901 tcgacctgaa cggagacggc gatctgctcg acaccgtgcg tttctatacg ccgaacacga
      961 cgaacaccaa tcgctacggc gccaccgcct cgctgatctg ggacctcacg gacgatcatc
     1021 ttctgcgctt cgcctttacc tacgaccacg cgagccatcg tcagacggcc cagtggggac
     1081 cgatgagttt tgaggacggc gaaccgcagg atgtgttcgc gggccgcgag ggcgaccgcg
     1141 tctatgccgc cgacggcgac atcatccgcg gccgtgaccg ttactcggtg gccgagctga
     1201 cccagtacgc gctcgaatat cgcgggcagt tcctcgaaga caagttcacg accacggtcg
     1261 gtttgcgcgc gcccttcttc aagcgcgacc ttaaccagta ctgctacacg ccgaacggcg
     1321 gttccggtag ttcgggcacc atccaggccg ctggcggcgc tttgtgcacg tcccgggccc
     1381 cgctgacgac gctgccgaat ggcaacgtca ccttcgcgcc cgcgccgatg ggcggcacgc
     1441 cggtggcgtt catcccgccg tatagcgaga cggtggaatt cgacgacctg ttgccgaacg
     1501 ccggtctttc gttccgccct tgggaccgcc atcagttcta cctgtcgtac gcggaaggcc
     1561 tgtcggctcc gcgcaccgac aacctgtatt cggtagttcg taatccgggc cagaccgaag
     1621 tcacccgtcc gacgccagaa tcggagatga cgaagtcgat cgatctcggc tggcgcctca
     1681 accatccgac gaatatcgcg tcgctggcgc tctaccacat cgactacacc aatcgcatcg
     1741 tgtcgacgtt caacgaggcc ctcggctaca gcgaagaccg caacgtgggt gacgtcaaga
     1801 tccagggctt cgatgcgcag gccggccatc gcttcggcga gtcgctgacg ctcacggtgt
     1861 ctgcgtcgta caacgacagc gaactcgagg gcagcctgaa tccgctgctc gatggcaagc
     1921 aactcgtgga gacgccggag tggacgtact cggcgcgcat ggatctcgac gttaccgaag
     1981 acttctatgt cggcctgcag gcgaagaagg tgggcgaccg cttcgcgacg gacaacaacg
     2041 acgagattgc gccgggctac accgtggtcg acatcgacct caactacggc ttcaagctgt
     2101 ggggcatgga acgcgccgag ctgcagctga acgtgacgaa tctgctcgac gaagagtact
     2161 tcggcaacat cagctcgggc acgggcggca cctcggtggg tttctattcc atcggcgcgc
     2221 cccgtaccgt agtggcgtcg atccgcttcg acttctgaaa tcggcccgga cgtgcgtagc
     2281 gtcctggctt tcctcctcct cgcggcggtg ggcattctgc ccgccgccgc ggcggatcgc
     2341 ccggccgggc tggtccgcat cgccaccttc aactgcagtc tgaatcgcgc ggccctgggc
     2401 gacctgcgcc gcgacctcgc gacgcccgac aacgtgcagg cgcgcgcggt ggcggagatc
     2461 atccagcgcg tgcggccgga catcctgttg ctgcaggaat tcgattacga cgcagaaggt
     2521 gcgtcgctgc gcgacttcca ggctaactat ctcgggcggc cgcagaatgg cgccgaggcc
     2581 atccagttcg cgcattcctt cttcaccgag tcgaacaccg gccagccttc cggcttcgac
     2641 ctgaacaacg acggcgaggt gggtggcgga caggacgcgc tcggcttcgg cgagtttccg
     2701 ggccagtacg ccatggcgct gctttcgcgc ttccccatcg atcacgcgca ggcacgaaca
     2761 ttccgcaagt tcgtctggcg tgacatgccg ggcgccatgc tgccggcgga ctggtattcg
     2821 gcgcaggaac tggcggtgct gccgctatcg tccaagagtc actgggatgt gccggtgcgt
     2881 atcgggaagc acacgctgca tctgctcgcg agccatccga cgccgcctgc attcgacggg
     2941 ccggaagacc gcaacggcaa gcgcaatcat gacgagattc gtttctggaa cgactacctg
     3001 accaaggcga acgcgggtta catccgcgac gatgccggca aacgtggcgg ctttcgcggc
     3061 aaggcgttcc tgatcatggg cgaccagaac tccgatccgg tggatggcgc cagcatccac
     3121 gggggcatcg gcgcattact cgcgcatccg cgcgtcgact cgagcttcgt gccgcagagc
     3181 gccggggcag cagaggccag cgccgtgcag ggtggcgcga atgccgaaca acgcggcgac
     3241 ccgcacaccg acaccgccga cttcaacgac cgcgtggccg gcaatctgcg cgtcgactat
     3301 ctattgccgt cgaaggggct gcgggtatgc gatggcggcg tgtactggcc agcgcaagcg
     3361 gagccggccg ccacactggt gtggggcgac cgcccggcgc cgagctcgga tcatcggctc
     3421 gtgtggctcg acgtcagcgt atccggagct cgatgcccac cgggcagtga tccgacaacc
     3481 agtgggtctt cgcatcctgg tcgctgaaaa cca
//
LOCUS       DATDEU010000360         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_796599, whole genome shotgun sequence.
ACCESSION   DATDEU010000360 DATDEU010000000
VERSION     DATDEU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796599"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VIV63_08535"
     CDS             <1..270
                     /locus_tag="VIV63_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6124683.1"
                     /translation="KTADGIAAYVNYCPHAGHPLIFRPHKFLTPDRNLILCASHGALF
                     ARDNGLCIAGPCPGQSLTPVPVEVMNGYVLLAEGVDVERLANAPL"
     gene            complement(293..604)
                     /locus_tag="VIV63_08540"
     CDS             complement(293..604)
                     /locus_tag="VIV63_08540"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HEY6124684.1"
                     /translation="MKPTIGNWYRIQGSDSFEVVAFDEDDGTIELQYFDGTVEEMDLE
                     DWQAAQEGGALEEIEPPEDWTGSVDVDPEEDDARGTVGYDKEDDRRQSAGKLEGLALF
                     E"
     gene            664..1350
                     /locus_tag="VIV63_08545"
     CDS             664..1350
                     /locus_tag="VIV63_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY6124685.1"
                     /translation="MLLRKSFTYKNEYLAQSTMLEQALKFFIVFFVVVEPISLIPVFS
                     GLTEGASNAYKHKMALKSVLVASGILLLFAVVGAGFLGAMGISIDSFRIFGGLLLFLI
                     ALEMVFARESGTRTSSEEKVESKNRADISVFPLAFPFMAGPGALTTLLLWFGPIPVTE
                     HPVQFLVMFSCALVVLALCLLAMWVSGPLMRVIGVTGTNVANRLFGVVLGALAVQFVI
                     DGLRASFTGL"
     gene            1360..>2067
                     /locus_tag="VIV63_08550"
     CDS             1360..>2067
                     /locus_tag="VIV63_08550"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEY6124686.1"
                     /translation="MRVLSFTRVLVPLLLVALARPLLAADCPPAGSDRAALDALKASK
                     FELSDDAQRQKLALALLPCLSSSDASLRDGIAFEAYYSWMRANQLDVRTRTELLARLS
                     GMIEATPPDALGFRQPFAALVLSEVARTDRIDAWLTPAQRAALVDSAARYVISVRDSR
                     GFDSKEGWRHGVAHGADLLTQLVFNSAVDRQGVDRLLGAVAAPVAPAGEHSYIDGESE
                     RLARPVLFAAQRGLYTSD"
BASE COUNT          317 a          653 c          636 g          461 t
ORIGIN      
        1 aagaccgcgg atggcatcgc cgcctacgtc aattactgtc cgcacgccgg acatccgctc
       61 atcttccggc cgcacaagtt cctgacgccg gaccgcaacc tgatcctgtg cgcttcacac
      121 ggcgcgctgt tcgcacgcga caacggactg tgtatcgccg gcccttgtcc cggacagtcg
      181 ctgacgcccg tgccggtgga ggtaatgaac ggatatgtct tgctggcgga gggcgtcgac
      241 gtggagcggc tggccaacgc gccgctgtag ttaggccagg cggactcgcg tcttactcga
      301 agagagcgag accttcgagc ttgccggcgc tctggcggcg gtcgtcttcc ttgtcgtaac
      361 ccacggtgcc ccgcgcgtcg tcttcttcgg ggtcgacgtc gaccgagccg gtccagtctt
      421 cggggggttc gatttcttcg agggcgccgc cttcctgtgc ggcctgccag tcttcgagat
      481 ccatctcttc aactgtgcca tcgaagtact gcagctcgat ggttccgtcg tcttcatcga
      541 aggcgacgac ttcgaatgaa tcgcttccct gaatgcggta ccagttgccg atggtgggtt
      601 tcatcgaatc cctccgaatc gtgatgctgc tatgctgctc gcgtcatttc ccgagccgac
      661 ttcatgttgc tccggaaaag tttcacttac aagaacgaat atcttgcgca aagcaccatg
      721 ctcgaacagg ccctgaagtt tttcatcgtg ttcttcgtcg tggtcgaacc gatctcgctg
      781 attccggtgt tctccgggct gaccgaaggc gccagcaacg cctacaaaca caagatggcc
      841 ctgaagtcgg tgttagtcgc aagcggaata ctgctgctct ttgcggtagt tggtgccgga
      901 tttctggggg ccatgggcat ctccatcgac tcgttcagga tttttggcgg cctgttgctt
      961 ttcctcatcg cgctcgagat ggtcttcgcg cgagaatcgg gaacgcggac gtccagcgaa
     1021 gagaaggtcg agagcaaaaa ccgcgcggac atttcggttt tccctcttgc attcccgttc
     1081 atggctggtc ctggtgcgct gacaacgctg ctgctctggt ttggacccat tcccgtgacc
     1141 gaacatccgg tccagttcct cgtgatgttc tcgtgcgcgc tggtggtgct cgcgttgtgc
     1201 ctgctcgcga tgtgggtctc gggaccgctg atgcgtgtca tcggtgttac cggcaccaac
     1261 gtcgcgaacc gcctgttcgg tgtcgtgctg ggcgcactgg ccgttcagtt tgtcatcgat
     1321 ggcctacgcg ccagcttcac gggactgtga agacgggccg tgcgggttct gtcattcaca
     1381 cgagtgctgg tcccgctgtt gctggtcgcg ctcgcgcgtc cactgctcgc cgcggattgc
     1441 ccgcccgcgg gatctgatcg ggccgcgctg gacgcgctga aggcgtcgaa gttcgaactc
     1501 tccgacgacg cgcaacggca gaagctggcg ctcgcattgc tgccgtgtct gtccagcagc
     1561 gatgcgtcct tgcgcgacgg catcgcgttc gaggcgtatt actcctggat gcgcgcgaat
     1621 cagctcgacg tgcgaacacg caccgaacta ctcgcgcgac tcagcggcat gatcgaggca
     1681 actccgcccg acgccctggg ttttcgtcag ccattcgcgg cgctggtgtt gtccgaggtg
     1741 gcgcgcaccg atcgcatcga cgcctggctc acgccggcgc agcgggccgc gctcgtcgac
     1801 agtgctgcgc gctacgtcat ctcggtacgc gactcccgcg gcttcgattc gaaggaaggc
     1861 tggcgtcatg gtgtcgcgca tggcgccgat ctgctgaccc aactcgtgtt caattccgct
     1921 gtcgaccgcc aaggcgtcga tcgcctgctc ggcgcggtcg cggcgccggt ggcgccggcc
     1981 ggcgaacact cttatataga tggcgaatcc gaacgcctcg ctcgcccggt gctgtttgcc
     2041 gctcaacgcg gcctgtacac gtcggac
//
LOCUS       DATDEU010000361         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1379876, whole genome shotgun sequence.
ACCESSION   DATDEU010000361 DATDEU010000000
VERSION     DATDEU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379876"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..361
                     /locus_tag="VIV63_08555"
     CDS             2..361
                     /locus_tag="VIV63_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009698244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HEY6124687.1"
                     /translation="MRYTARVSTLPPCPKCQSAFAYEDGVMLVCPECAHEWSKDAAAP
                     VIEGVAEVRDAHGNVLSDGDSVTVIKDLKVKGSSSVVKVGTKVKGIRLVEGDHNIDCR
                     IEGIGEMKLKSEFVKKA"
     gene            complement(414..1046)
                     /locus_tag="VIV63_08560"
     CDS             complement(414..1046)
                     /locus_tag="VIV63_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124688.1"
                     /translation="MKLSRFLAIAGLAAAGTAAAGGGYSSTLFKFDGGTGNQVFRSAA
                     GVPTLNTVAGVAPGGAPWGITSFDATIKTNGDIRARGEGVLLWGGDGLGTRAGPRQVI
                     LSLFCRNVPVPPAVSAALILTPFNSEPVDLDEDGDFVVRGKLTDATGATPPLTCGDTI
                     DNRPVLLIRSVTPANPTTGAPATPGAWFAAGLLAGKHRDGHGGKGDWDRY"
     gene            complement(1131..>2195)
                     /locus_tag="VIV63_08565"
     CDS             complement(1131..>2195)
                     /locus_tag="VIV63_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6124689.1"
                     /translation="KREVLVSLSYQANSDVALYDAQGVMVLYAGRHAPELTPKQIAES
                     NWMMTNRGLTFNRPLADGSHLVVRPRHRILAYGLHMGLLLTTIAALLALLTYPLTRGI
                     TARLARLQTGVLQFGAGNLAARVKVEGRDEVAMLATSFNESAEHIEKLVRAHQMLLAN
                     CSHELRTPLARLRMGIERLAGTDPNTNAELARNIAELDALIGEMLLSSRLDASSGVER
                     TETVDLLALAAEEAAHFDLQASGEALIVKADATLLRRLVRNLLENARVHAGGATDIRI
                     EGDTQHARIVIEDAGQGVAYPDRDKIFEPFYRASTATHSSGAGLGLAIVRKIALAHGG
                     TVEYSPRDGGGSRFTVKLPR"
BASE COUNT          396 a          707 c          728 g          364 t
ORIGIN      
        1 aatccgatac actgcgcgcg tgtccaccct gcctccctgc ccgaaatgcc agtccgcctt
       61 cgcctacgaa gacggcgtga tgctcgtgtg tccggagtgc gcacacgaat ggtcgaaaga
      121 cgccgcagcg ccggtgatcg agggagttgc cgaagtgcgc gatgcgcatg gcaacgtgtt
      181 gagtgacggc gacagcgtca cggtcatcaa ggacctgaag gtgaaaggct cctcttcggt
      241 cgtgaaggtg ggcacgaaag tgaagggcat ccgcctcgtc gaaggcgatc acaacatcga
      301 ttgccgcatc gaaggcatcg gcgagatgaa gctgaagtcg gagttcgtga agaaggcctg
      361 aagaaaatgc gccggcgatc cttcgccggc gcagccctga caagaacgaa ggatcaataa
      421 cgatcccagt cgcccttacc accatggccg tcgcgatgct tgcctgcaag caggcccgcg
      481 gcaaaccagg cgcccggagt cgccggcgca cccgtggtcg gattcgccgg agtaacggaa
      541 cggatcagca acaccggacg gttgtcgatg gtgtcgccac aggtgagagg cggcgtcgcg
      601 ccggtcgcat cggtcagctt gccgcgaacc acgaagtcgc cgtcttcatc gaggtcgaca
      661 ggctcggagt tgaacggcgt gaggatcagc gcggccgaca cggctggcgg caccggcacg
      721 ttgcggcaga acagcgagag gatcacctgg cgcggaccgg ctcgtgtgcc gaggccatca
      781 ccaccccaga gcaacacgcc ttcaccgcgc gcgcgaatgt cgccattggt cttgatggtc
      841 gcgtcgaatg acgtgattcc ccacggcgcg ccgcccggcg ccacgccggc aacggtgttc
      901 aacgtgggaa cgccggccgc ggaacggaac acctggttgc ccgtgccgcc gtcgaatttg
      961 aagagggtcg aagaatagcc gccgcccgcg gccgcggtcc ccgccgcagc caggcccgcg
     1021 atggccagaa aacgagagag tttcatgtga ggctcctgcc ttgattgtta ttacggcagt
     1081 gagtagcgcg ccttcgcgac ttggttccgc gagcggcgga tgccggactc tcagcgcggc
     1141 aatttcaccg tgaagcggct gccaccgccg tcgcgcggcg agtactccac ggtgccgcca
     1201 tgcgcgagcg cgatcttccg cacgatggcc agcccgagcc cggccccgct cgaatgcgtg
     1261 gccgtactcg cgcgatagaa cggctcgaag atcttgtcgc gatcgggata ggcgacgccc
     1321 tgccccgcgt cttcgatcac gatgcgcgcg tgttgggtat cgccctcgat gcggatgtcc
     1381 gtggcgccac ccgcatgtac acgcgcgttt tccagcagat tgcgcacgag ccggcgcaac
     1441 agcgtcgcgt cggctttcac gatcagcgct tcgccgctcg cctgcaggtc gaagtgcgcg
     1501 gcctcctccg ccgcgagcgc gagcagatcc accgtctcgg ttcgctccac gccactcgac
     1561 gcatcgagcc ggctggacag caacatctcg ccgatgagcg catcgagctc ggcgatattg
     1621 cgcgccagtt cggcgttggt attggggtcg gtgcccgcga gacgctcgat gcccatgcga
     1681 agccgcgcca gtggtgtgcg cagctcatgc gagcagttgg cgagcagcat ctggtgcgca
     1741 cgcacgagtt tttcgatgtg ttcggcggac tcgttgaagc tggtcgcgag catggccact
     1801 tcatcgcggc cctccacttt cacgcgcgct gccaggttgc cggcgccgaa ctgaagtacg
     1861 cccgtctgca gccgggcgag gcgcgcggtg atgccccgcg tgagtggata ggtgagcagc
     1921 gcgagcagcg ccgcgatggt cgtcagcagc aaccccatgt gcaggccgta agccaggatt
     1981 cggtgccgcg gacgcacgac caggtgactg ccgtccgcaa gcgggcgatt gaaggtcagc
     2041 ccccggttgg tcatcatcca gttggattcg gcaatctgct ttggcgtgag ctctggcgcg
     2101 tggcggccgg cgtacaggac catcacaccc tgtgcgtcgt agagcgcgac atcggaattc
     2161 gcctggtaac tcaacgagac gagcacctcg cgctt
//
LOCUS       DATDEU010000362         3266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_650739, whole genome shotgun sequence.
ACCESSION   DATDEU010000362 DATDEU010000000
VERSION     DATDEU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650739"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VIV63_08570"
     CDS             <1..261
                     /locus_tag="VIV63_08570"
                     /inference="COORDINATES: protein motif:HMM:NF025769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HEY6124690.1"
                     /translation="PWSPRFSDMAKKAGMTLDDLANKVDVPGHRGPHPQSYHQAVFDR
                     LTGATRGLSGDAYSSAFRAELAAIGRDASTAGHALNKLLTGW"
     gene            261..821
                     /locus_tag="VIV63_08575"
     CDS             261..821
                     /locus_tag="VIV63_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1629 domain-containing protein"
                     /protein_id="HEY6124691.1"
                     /translation="MRYFRLNDDIASVDRWHLGDASANSGTLPDLLDVRPLPAELTLS
                     VPVTRPGRKLVFCFTSFGVPVAVRELADLILSIAQSEVQIVRIDILGDGYRCEYRVPH
                     CLREYECLDESKSEFLKWTEANGRPELVGHYRMVTRMRIDRRMLPNDAHFFRVAKWPV
                     VLVVSEVVKQAMERAGSVGAIFDEIS"
     gene            1324..>3266
                     /locus_tag="VIV63_08580"
     CDS             1324..>3266
                     /locus_tag="VIV63_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HEY6124692.1"
                     /translation="MGAAVNAVAQAPAIQYAEPVALTLKASNAQFDAYGRRFSLTLVD
                     NDRVLDKLSAQRKQQLQSYKLKRGSLDGAPGSWVRLTESPSGVEGAIWDGHDLYTVTR
                     YDRISPYLTTPLDAAPGQTVVYRLSDAIDVLPRDFCALADDMVMSKAANGLEQYQAVV
                     REITAGVFAPQLTRQIEISLIGDTDFQAAESADPTAAMLARLNIVEGIFSEQVGLLIL
                     ATDVRLMPAQADPFTATKGATLLEQLGAYRKNTPAVAARGLAHLMTGKDLDGTTAGIA
                     YVRTVCDVERGVSVSSRSFGTTISALIMAHELGHNFGAEHDGEAGTTCASTGGGFIMA
                     PSVSGYATFSQCSINTMEPVISAAECVTPAQYADVSVEAAVTSVAGEGGLPFTLPFVV
                     RSTGNTTAADATLTVTLPANAAYSIDSASSSLGSCSVSGLTATCSFGAMTGETSAQVN
                     VVARSTSAANFFAQARVTASNDRITSNNNRQIGVLIRSGIDAALTLSTSTTEVALGSS
                     IEVYADVSSLRAMPLRNALLSLSLNQTVVSASMPGATCTTNASSVSCTITDLPAGATR
                     RLTVQAATQVAGSLFANASVSATGDGDITNNGASTTAWVQAERDIELTAGPAVVDLGV
                     GTIYSVPYTVRSRGPLPTGDVTL"
BASE COUNT          591 a         1024 c         1031 g          620 t
ORIGIN      
        1 ccctggagtc caaggttttc tgatatggca aagaaggccg gaatgacact tgacgattta
       61 gcgaacaagg tagatgttcc cgggcatcgt gggccacatc cgcagtccta ccatcaagcg
      121 gtgttcgacc gcttaacagg cgcaactcgt ggtttgtctg gagacgcgta ctcgtcagca
      181 tttcgcgcgg aactcgccgc gattgggcgt gacgcctcta ctgctggaca tgcccttaac
      241 aaactactta ctggctggtg atgcgctatt ttcggttgaa tgatgacatc gcgtcagttg
      301 atcgatggca cttgggcgat gcgagcgcga actcaggaac acttcccgat ctccttgatg
      361 tgcgtccgct gcccgccgaa ctaacgcttt cagttccagt gactcgtcct gggcgaaagc
      421 tggtcttttg ctttacaagt tttggggtgc ctgtcgccgt tcgagagctc gcggacttga
      481 tcttgtcgat tgcacaatcg gaagtgcaaa ttgttcgaat tgacattcta ggtgacggct
      541 accgttgcga gtatcgcgtt ccgcactgcc tgcgcgaata tgaatgtctg gacgaatcta
      601 agtctgagtt cttaaagtgg acggaggcga atggcaggcc cgagctcgtt ggccactacc
      661 gaatggtgac gagaatgcgc atcgatcgga gaatgttgcc aaacgacgcg catttcttcc
      721 gggtcgcgaa gtggcccgtg gtgttagtcg tatcggaagt cgtcaagcag gcgatggaga
      781 gagccggaag cgtcggagca atttttgacg agattagtta gccaacgact caacggctgc
      841 cgcgaagagc gaggagtgtt gttggcttca ctcaggtttt ctcgtcttga cttgtcggct
      901 ttcacggcca gcaacctacg cctgagaatc ttttcgaagc gattcgcagt ggtattctga
      961 atcgtcagcg gcctcccgat tgcacctgct tgtggacggc ggaatgattc tccatttgca
     1021 ctcacgctca tgagatggtc caggagcgtg gacgtaacgg catagcgcca agaaacgcga
     1081 tgaaataaac gtcgctttcc atgcgcgtta tgtccacgcg ttatgttcct gtggcccgcg
     1141 tcacttaatc gcgatcccac gcgggttcac tcccccgcct acgcaaaccc accggctaaa
     1201 gtcacttctc cgccagcgcc agttctgtaa cttgagcgct ccggagaaga acaacgccaa
     1261 gaaggcgctg ggggctttag ggtgcgagcg atcggcagcc ggtttgtgct gtggtttttg
     1321 gcgttgggag cagcggttaa cgccgtcgcc caggcgcctg ccattcagta cgccgagcct
     1381 gtcgcgctga cgctcaaggc cagcaacgcg caattcgacg cttacggccg ccgcttctcg
     1441 ctgacgctgg tcgacaacga ccgcgtgctg gacaagctct ccgcgcagcg caagcaacaa
     1501 ctccagtcct acaagctcaa gcgtggttcg ctcgacggcg cgcccggctc gtgggtgcgg
     1561 ctcaccgaaa gtccctcggg tgtcgaaggc gcgatctggg acggacacga cctctacacg
     1621 gtcacgcgct acgaccgcat ttcgccgtac ctcacgaccc cgctcgacgc agccccgggc
     1681 cagaccgtgg tgtatcgcct gtcggatgcc atcgacgtgc tgccgcgcga cttctgcgcg
     1741 ctggcggacg acatggtgat gtcgaaggcg gccaacggcc tcgagcaata ccaggcggtg
     1801 gtgcgcgaga tcacggcggg cgtgttcgcg ccacagctca cgcgtcagat cgagatctcg
     1861 ctgattggcg ataccgactt ccaggccgcc gaaagcgccg acccgaccgc cgcgatgctg
     1921 gcgcgcctca acatcgtcga aggcatcttc agcgagcagg tgggcctgct gatcctggcc
     1981 accgacgtgc gcctgatgcc ggcgcaggcg gatcctttca ccgcgaccaa gggcgccacg
     2041 ctgctcgagc agctcggtgc gtatcgcaag aacacaccgg ccgtcgccgc gcgcggcctc
     2101 gcgcacctga tgacgggcaa ggacctcgac gggaccaccg ccggcatcgc ctacgtgcgc
     2161 acggtgtgcg acgtggagcg cggcgtgtcg gtgagctcgc gctcgttcgg caccacgatt
     2221 tccgcgctga tcatggcgca cgagctgggc cacaacttcg gcgccgagca cgacggcgag
     2281 gcgggcacga cctgcgcgag caccggcggc ggtttcatca tggcgccctc ggtcagcggc
     2341 tatgccacgt tctcccagtg cagcatcaac acgatggagc cggtgatctc ggcggccgag
     2401 tgcgtgacgc cagcccaata cgccgacgtg tcggtggaag ccgccgtcac atcggtggcg
     2461 ggcgagggtg ggctgccatt cacgctgccg ttcgtggtcc gctccacggg caatacgacc
     2521 gcggccgatg cgacgctgac ggtcacgctg cctgccaacg cggcgtacag catcgattcc
     2581 gcgtcgagct cgctcggcag ttgctcggtc tcgggcctca ccgccacctg ctcgttcggt
     2641 gcgatgaccg gcgaaaccag cgcgcaggtg aacgtggtcg cgcgcagcac ctcggcggcg
     2701 aacttcttcg cgcaggcgcg cgtgacggcc agcaacgacc gcatcacctc gaacaacaac
     2761 cggcagatcg gggtgttgat ccgctcgggc atcgacgcgg cgctgacgct gtccaccagc
     2821 acgaccgaag tggcgctggg ctcgtcgatc gaggtatatg ccgacgtgag ttcgctgcgc
     2881 gcgatgccgc tgcgtaatgc gctgctgtcg ctcagcctga atcagaccgt ggtctcggct
     2941 tcgatgcccg gcgccacgtg caccacgaac gcttcgtcgg tgagctgcac gatcaccgat
     3001 ctgcccgccg gcgccactcg ccggctcacg gtgcaggccg cgacgcaggt ggcgggttcg
     3061 ctgttcgcga acgccagcgt gagcgcgacc ggtgatggcg acatcacgaa caacggcgcc
     3121 agcaccacgg cctgggtgca ggcggagcgc gacatcgaac tcacggcggg ccctgcggtg
     3181 gtcgatctcg gcgtgggcac catatatagc gtgccgtaca cggtgcgttc gcgcgggccg
     3241 ctgcccacgg gcgacgtcac gctgaa
//
LOCUS       DATDEU010000363         6811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_66685, whole genome shotgun sequence.
ACCESSION   DATDEU010000363 DATDEU010000000
VERSION     DATDEU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66685"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..679)
                     /locus_tag="VIV63_08585"
     CDS             complement(89..679)
                     /locus_tag="VIV63_08585"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY6124693.1"
                     /translation="MAKLYFYYSTMNAGKSTALLQAGHNYEERGMRTLLYTARIDSRD
                     GGRIHSRIGIEREALHFYPQLDLREQIYAENKKAALACVLVDESQFLTPTQVKQLGAV
                     VDELNVPVLCYGLRTDFRGELFPGSAQLLAWADNLVELKTICHCGRKAIMVVRVKADG
                     TAEREGAQIEIGGNERYVPLCRRHFSQALAGAKIPA"
     gene            complement(683..1930)
                     /locus_tag="VIV63_08590"
     CDS             complement(683..1930)
                     /locus_tag="VIV63_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY6124694.1"
                     /translation="MLLIQGLLGIVVFMVLAMPFSSNWRRINLKLVAFAIALQFIICA
                     LLLKAPGISEGLLYVNRAVGALGRATMKGTTFVYGYLGGGPPPFEVTNPGFLTTFAFQ
                     VLPLVIVMSALSALLWYWRILPIIIKGISVIFERALGTRGPAGLVATANIFMGQIEAP
                     LLVRPYLARMTQYELLLVMTAGMATIAGSVMVIYSAMLGEQINGVLGQLITKSIMSVP
                     AAILFAHLIIPTENDSSQELEKPPRVYESSMDAITRGTSDGLNIYLNILAILIVAIAL
                     VALLNGMISFIEVGGAPLTMERVAGWVFAPIAWLMGIPWSESTTVGSLLGIKTVLNEF
                     IAYLTLQDMPAGTLSEHSKLIALYAVCGFANLSSVGIQISGIGAMCPERRNDLARLGF
                     RALLAATLASCMCGAVVGIVTTF"
     gene            complement(1952..2884)
                     /locus_tag="VIV63_08595"
     CDS             complement(1952..2884)
                     /locus_tag="VIV63_08595"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEY6124695.1"
                     /translation="MPTTIPTLDARNTESPQFAQDIAAAYAEFGFVIIENHGIDKALI
                     DQCLECFQRFFALSAEEKLQYKIPNGGGARGYTAFGIETAKGAKHHDLKEFWHVGREL
                     PPGHPFNEVMAPNIWVDTIPGFRESTLGLYRAFDALGLRLLSAIARSVALPANFFDDK
                     VDLGNSVLRIIHYPPMPPEPTPSVRAGAHEDINVITLLLGAEEPGLEVLSRKGEWIAV
                     NPPPGSLVCNVGDMLQRLTNMHLRSTTHRVTNPPRDRASNARYSLPFFLHFNPDFLIK
                     TLPQYVDAQHPDQFPVPINAHDFLQERLREIKLI"
     gene            2989..3903
                     /locus_tag="VIV63_08600"
     CDS             2989..3903
                     /locus_tag="VIV63_08600"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEY6124696.1"
                     /translation="MVRPKHFGFNAQTAGTNRFQQMGPDGRGVAADVAAHALREFDAF
                     AAALSAEGVKVCVVPDSESPRKPDAVFPNNWVSFHADGTVVLYPMHAENRRAERRPEI
                     VEAVARETGFQVRRVLDLTRHEKEGRFLEGTGSLVLDHRARVAYACRSPRTDETVARE
                     WARELGFELELFDATDQSGTPIYHTNVLMYVGTRAAVVALEAIAPADRDRVGARLSAA
                     GIDVIAIDYTEMGAFAGNMLEVGSWDEYLGDFTILVMSETARHALATPKYVRLYSSVD
                     AVLAVPIEVIERHGGGSVRCMLAEVFLP"
     gene            3921..4568
                     /gene="plsY"
                     /locus_tag="VIV63_08605"
     CDS             3921..4568
                     /gene="plsY"
                     /locus_tag="VIV63_08605"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY6124697.1"
                     /translation="MDFPAKVLLAYLLGSIVGSLVVGRLWGGVDIRTSGSGNAGATNA
                     LRTQGAAFATWVIIIDVAKGWIAAALLPGLVLPGIAPSPPELAAWLPPACALAAILGH
                     VFPVWHGFRGGKGVATLVGAFAGIDLLLLVPLFVSWLAVVTVSGYVGLASITASFAIP
                     VYLLLRDGPVMTPVLGFALACAALVVYTHRANVRRMRAGTEPRARRLWLFGRGRA"
     gene            4565..5572
                     /locus_tag="VIV63_08610"
     CDS             4565..5572
                     /locus_tag="VIV63_08610"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY6124698.1"
                     /translation="MSGATSQGPLPQRVFQRLDDRGFLSGETLATDLSVTRAAVWKAV
                     EQLRELGVALDAQSNKGYRLAPGVSALSVDRIQSLLPADVRSHLEVLLVEWTLDSTNT
                     RLLEALPPAAGTAAVVLAEHQSGGRGRRGRGWVAPPGGAICLSLAWQYADMPVDLSAL
                     SLVVGLCAVNALTSLGVPGVALKWPNDLVTQAGKLGGILIEMRAESGGPVHVVAGIGL
                     NVVLDDAARETVKATGNIADDIRANCPAVPDRNAIVAALLARLIPAFEGFPRHGLTPH
                     LDNWHACDALRDREVRVENAGEITRGIARGVDAHGALLVESPAGVRRFISGEVTVRVE
                     S"
     gene            5569..6321
                     /locus_tag="VIV63_08615"
     CDS             5569..6321
                     /locus_tag="VIV63_08615"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY6124699.1"
                     /translation="MTTLLVDIGNTRVKWATLRGARQGRMQAAAHENSGLALRALVRG
                     APRDVSRVVVVSVVDEALTRVLDAAAQRRFGVAPEYIKSTQRAFGVINSYRDTWRLGA
                     DRWVAAVGAHAMARGRTAVIANVGTALTIDAVTASGRHRGGAIAPGPRTMVESLLSGT
                     HGIRRRAQGGRGVSRSLFATDTASALAAGSVFAAAAFVDRALLEAQQKLRGRPLLILT
                     GGGAPELKRYLTSPVQMVPDLVLRGLAVFAGT"
     gene            6354..>6811
                     /locus_tag="VIV63_08620"
     CDS             6354..>6811
                     /locus_tag="VIV63_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124700.1"
                     /translation="MRAALLLLLLANLAFFAWMRWLAPGEAQMPVSPKVQAPPLELVR
                     EARAATANGGCVTVGPFATNELAARGRQTLADAGYTSIPREVESRVFDGYWVYLEAPP
                     TESGERRLLDRLKKGGVPEAAAMGDFGARRISLGIFSEESRAVAQSERVAR"
BASE COUNT         1090 a         2206 c         2299 g         1216 t
ORIGIN      
        1 gtggccgcta tggggcagaa tttccgtcct tgctacctga gcgtgcagta cgaatcttga
       61 gcatttgctg cgcttttgcg gcagcgcctc aggcaggaat ctttgcgccg gcaagagctt
      121 gcgaaaaatg tctccggcac agtggcacgt agcgctcgtt gccgcctatc tcaatctgcg
      181 caccttcgcg ttcggcggtg ccgtcggcct tgacacgaac caccatgatg gccttccgac
      241 cgcaatggca gatggtcttg agctcgacca ggttgtcggc ccaggcgagc aactgggcgc
      301 tgcccggaaa aagctcgccg cggaagtccg tgcgcaggcc gtaacacagc accggaacgt
      361 tcaactcgtc gaccaccgcg ccgagttgct tgacctgcgt cggcgtgagg aactgcgatt
      421 cgtcgacgag cacgcaggcc agcgcggcct tcttgttctc ggcgtaaatc tgctcgcgca
      481 gatccagctg cggatagaag tgcagcgcct cgcgctcgat gccgatgcgc gagtggatgc
      541 gtccgccgtc gcgggagtcg atacgcgccg tgtaaagaag agtgcgcatg ccgcgctctt
      601 cgtagttgtg cccggcctgc agcaacgcgg tggatttacc cgcgttcatg gtcgagtaat
      661 agaagtagag cttggccacg aatcagaacg tcgtgacgat gccgaccacg gcgccgcaca
      721 tgcacgaggc cagcgtggcc gcgagcaacg cacggaaccc caggcgcgcc agatcgttgc
      781 ggcgttcggg gcacatggcg ccgatgccgg aaatctggat gcccacgctc gagaggttcg
      841 cgaagccgca gaccgcgtag agcgcgatga gcttgctgtg ttcggacaac gtgccggcgg
      901 gcatgtcctg cagcgtcagg taggcgatga actcgttgag cacggtcttg atgccgagca
      961 gcgatcccac ggtggtggac tcgctccagg gaatacccat gagccaggcg atgggcgcga
     1021 agacccagcc ggcgactcgc tccatggtga gcggcgcgcc gcccacctcg atgaagctga
     1081 tcatgccatt gagcaatgcc accagcgcga tggccacgat gaggatggcc aggatattca
     1141 ggtagatgtt gaggccgtcg gaggtgccgc gcgtgatcgc gtccatcgag ctctcgtaga
     1201 cgcgaggcgg cttttcaagc tcctgcgagc tgtcgttctc cgtggggatg atcagatgcg
     1261 cgaacaggat ggctgcgggc accgacatga tcgacttggt gatcagctgg ccgagcacgc
     1321 cgttgatctg ttcgccgagc atcgccgagt agatgaccat cacggagccg gcgatcgtcg
     1381 ccatgcccgc ggtcatgacc agcagcaatt catattgagt catgcgcgcg agatacggac
     1441 gcaccagcag cggcgcctcg atctggccca tgaagatgtt ggccgtggcc acgagacccg
     1501 ccggtccgcg cgtgccgaga gcacgttcga agatcaccga gatgcccttg atgatgatgg
     1561 gcaggatgcg ccagtaccac agcagcgccg agagcgccga catgacgatg accagcggca
     1621 gcacctggaa cgcgaaggtg gtcaggaagc ccgggttggt cacttcgaag ggtggcgggc
     1681 cgccaccgag ataaccgtag acgaacgtgg tgcctttcat ggtggcgcgg ccgagcgcgc
     1741 ccaccgcgcg attgacgtat agcagcccct cgctgattcc tggcgccttg agcagcagcg
     1801 cgcagatgat gaattgcagc gcgatggcga acgcgacgag cttgagattg atgcggcgcc
     1861 aattgctgct gaacggcatc gccagcacca tgaacacgac tattccgagc agaccctgga
     1921 tcaggagcat cgggagacct tcttatagtt gttagatgag cttgatttcc cgcagtcgct
     1981 cctgcaggaa atcatgcgcg ttgatcggta ccgggaactg gtcgggatgc tgcgcgtcga
     2041 cgtactgcgg cagcgtcttg atgaggaagt cgggattgaa gtgcaggaag aacggcaacg
     2101 agtaacgcgc gttgctcgcg cggtcgcgcg gcggattggt gacgcgatgc gtggtggagc
     2161 gcaggtgcat gttggtcagc cgctgcagca tgtcgccgac gttgcagacc agcgagcccg
     2221 gcggcggatt cactgcgatc cactcgccct tgcgcgacaa cacctcgagc ccgggctctt
     2281 cggcgcctaa caacaacgtg atgacgttga tgtcctcgtg cgcgccggcg cgcaccgacg
     2341 gcgtgggctc cggcggcatg ggcgggtagt ggatgatgcg cagcacgctg ttgccgagat
     2401 ccaccttgtc gtcgaagaag ttggccggta acgccaccga gcgcgcgatg gccgagagca
     2461 gtcgcagccc cagggcgtcg aacgcgcggt acaggccgag cgtcgattcg cgaaacccag
     2521 ggatggtgtc gacccagatg ttaggcgcca tgacctcgtt gaaggggtgt ccgggcggaa
     2581 gctcgcggcc gacgtgccag aactccttga ggtcgtggtg cttcgcgccc ttggcggtct
     2641 cgatgccgaa agccgtgtag ccgcgggcgc cacccccgtt cgggattttg tactgcagct
     2701 tctcctcggc ggagagggcg aaaaaccgct ggaagcactc gaggcactgg tcgatgagcg
     2761 ccttgtcgat tccgtggttt tcgatgatga cgaacccgaa ctccgcgtac gccgcggcga
     2821 tgtcctgagc gaactgcggg ctttcggtgt tgcgcgcgtc gagcgtggga atggtcgtgg
     2881 gcattggcgg ctattgtgcc taccgggttc aggttgtaga agtcggtaat gcagggtggg
     2941 aaagaacggg gccggtcggg cccttcgcag tgcgcgagcg ccatattgat ggttcgcccg
     3001 aaacacttcg gattcaacgc tcaaacggcg ggtaccaatc gttttcagca gatgggtccg
     3061 gatggccggg gtgtcgccgc ggacgtggcg gcccacgccc tgcgggaatt cgatgctttt
     3121 gccgccgcgc tgtccgccga gggcgtcaag gtgtgcgtgg tgcccgactc ggagtcgccg
     3181 cgcaagccgg acgccgtgtt cccgaacaac tgggtgagct ttcacgccga cgggaccgtg
     3241 gtcttgtacc cgatgcacgc cgagaaccgg cgtgctgaac gccgccccga gatcgtcgag
     3301 gccgtggcac gcgaaaccgg cttccaggtg cggcgtgtcc tcgacctgac gcgacatgaa
     3361 aaagagggcc gtttcctcga aggcaccggc agcctggtgc ttgatcatcg cgcccgcgtc
     3421 gcctacgcct gccgttcgcc acgcaccgac gagacagtgg cgcgcgaatg ggcgcgcgag
     3481 ctgggcttcg agctcgaact gttcgacgcc accgatcagt ccggcacgcc catctaccac
     3541 accaacgtgc tcatgtacgt cggcacgcgc gccgccgtgg tggcgctcga ggcaatcgct
     3601 cccgccgacc gtgaccgcgt gggcgcgcgg ctttccgccg ccggcatcga cgtgatcgcc
     3661 atcgattaca ccgaaatggg cgcattcgcc ggcaacatgc tcgaagtggg aagctgggac
     3721 gaatatcttg gcgacttcac gattctcgtg atgtcggaaa ccgcgcggca tgcgctcgcg
     3781 actcccaagt acgtgcgcct ctattcatcc gtggacgccg tgctcgcggt gcccatcgaa
     3841 gtgattgaac gtcacggcgg cggcagtgtc cgatgtatgc tcgcggaagt tttcctgccc
     3901 tagagtttcc gaattccgcc atggatttcc cggccaaggt cctgctcgcc tatcttctcg
     3961 gctccatcgt cggcagcctc gtggtcggcc gcctgtgggg cggcgtcgac atccgcacct
     4021 ccggtagcgg caatgcgggc gccaccaacg cgttgcgcac gcaaggcgcc gcctttgcaa
     4081 cctgggtcat catcatcgac gtcgccaagg gctggatcgc cgcggcgctg ctgccggggc
     4141 tcgtgttgcc gggcatcgcg ccttcgccgc cggaactggc ggcctggctg ccgcccgcct
     4201 gtgcactggc cgccatcctc ggccacgtgt ttccggtgtg gcacgggttt cgcggtggca
     4261 agggtgtcgc cacgctggtg ggcgccttcg ccggcatcga cctgctgctg ctcgtcccgc
     4321 tgttcgtgag ctggctcgcc gtggtcacgg tgtcgggtta cgtcggcctg gcctccatca
     4381 ccgcctcgtt cgccatccct gtctatctac tgctgcgtga cggtccggtc atgaccccgg
     4441 tgctcgggtt cgcgctggcc tgcgcggcgc tggtcgtgta cacgcatcgc gccaacgtgc
     4501 ggcgcatgcg cgccggcacc gagccgcgcg cccgccggtt gtggttgttc gggcgcggac
     4561 gcgcgtgagc ggcgccacct cacagggtcc tttgccgcag cgcgtattcc agcgtctcga
     4621 cgatcgcggc tttctgtcgg gagaaaccct cgccaccgat ctgtcggtca cgcgcgccgc
     4681 ggtgtggaaa gccgtcgagc agttgcgcga actcggcgtg gcgctcgatg cccagtcgaa
     4741 caagggttac cggctcgcac cgggcgtcag cgcgctgtcg gtcgatcgca tccagtcttt
     4801 gctgcccgcg gacgtgcgtt cgcatctcga agtgctgctc gtcgagtgga cactcgattc
     4861 caccaacacg cggttgctcg aggcgctgcc cccggccgcg ggcacggcgg ccgtcgtgct
     4921 cgccgaacat cagtcgggtg gaagagggcg gcgagggcgc ggctgggttg cgccgccggg
     4981 cggcgccatc tgcctgtcgc ttgcctggca atacgccgac atgcccgtcg atctgtccgc
     5041 gctgtcgctc gtcgtcgggc tgtgcgcggt gaacgcgctg acctcgctcg gcgtgcccgg
     5101 cgtggccttg aagtggccca acgatctcgt gacccaggcc ggcaagctcg gcggcatcct
     5161 catcgaaatg cgcgcggaat ccggcggtcc cgtgcacgtc gtcgcgggca tcggtctcaa
     5221 cgtggttctc gacgatgccg cgcgcgaaac cgtgaaagcc accggcaaca tcgccgacga
     5281 tatccgcgcg aattgccccg ccgtgccgga tcgcaacgcg attgttgccg ctctcctggc
     5341 gcgcctgatt cccgcgttcg aaggttttcc gcgccacggc ctcacgccgc acctcgacaa
     5401 ctggcatgca tgcgatgcac tgcgcgaccg cgaagtgcgc gtggaaaatg ccggcgagat
     5461 cacgcgtggc atcgcgcgcg gcgtcgacgc gcacggcgcc ttgctggtcg aatctccggc
     5521 cggcgtgcgg cgcttcatct ccggagaagt cacggtgcgt gtggaatcat gaccactttg
     5581 ttagtcgaca tcggcaacac ccgcgtgaag tgggccacgc tgcgcggcgc ccggcagggt
     5641 cgcatgcagg cggcggcgca cgagaattca ggtcttgcgc tgcgcgcgct ggtgcgcggt
     5701 gctccgcgcg acgtgagccg cgtcgtcgtg gtcagcgtcg tcgacgaagc gctcacgcgt
     5761 gttctcgacg cggccgcgca acgaagattc ggcgtcgcgc cggagtacat caaatcgacc
     5821 cagcgcgcat tcggcgtgat caacagctat cgagacacgt ggcggctcgg cgccgatcgc
     5881 tgggtcgcgg cggtgggcgc gcacgcgatg gcgcgtggcc gcacggccgt catcgccaac
     5941 gttggaaccg cgctcaccat cgacgccgtt accgcgagcg gacgccatcg cggtggcgcg
     6001 attgcgcccg gacctcggac catggtcgag agcctgctgt ccggcaccca cggcatccgc
     6061 cgccgtgcgc agggcgggcg cggcgtgtcg cgctcgttgt tcgccaccga taccgcgagc
     6121 gccctggccg ccggttccgt gttcgccgcc gccgcgttcg tcgatcgcgc gctgctcgaa
     6181 gcgcagcaga aactgcgcgg ccggccgctg ctcatcctca ccggtggcgg cgcgcccgaa
     6241 ctcaaacgct acctgacgag ccccgtgcag atggtccccg acctcgtgct ccgtggtctc
     6301 gccgtgttcg cggggacttg attgggtaag catttgctac actcgcgcct cgcgtgcgtg
     6361 cagccttact ccttctctta ttagcgaatc tcgcgttctt cgcgtggatg cgctggcttg
     6421 cgcccggcga ggcgcagatg cccgtgtcgc ccaaggtcca agcgccgccc ctggagctcg
     6481 ttcgcgaagc gcgcgcggcc acggccaacg ggggctgcgt caccgtgggc ccgttcgcca
     6541 ccaatgaact ggcagcacgc gggcggcaga cgctcgccga cgccggttac acgtccattc
     6601 cccgcgaagt cgaatcccgc gtgttcgacg gttactgggt ctacctcgaa gccccgccca
     6661 ccgaatccgg cgagcgccgt ttgctcgatc ggctgaagaa gggcggcgtc cccgaggccg
     6721 ccgccatggg cgatttcggc gcgcgccgca tctcgctcgg catcttctcg gaggaaagcc
     6781 gcgcggtggc gcagtcggaa cgggtggccc g
//
LOCUS       DATDEU010000364         2965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1671740, whole genome shotgun sequence.
ACCESSION   DATDEU010000364 DATDEU010000000
VERSION     DATDEU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671740"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="VIV63_08625"
     CDS             complement(<1..1924)
                     /locus_tag="VIV63_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY6124701.1"
                     /translation="MSLRRPSAAKWRIAAAFCALLFTSISTFAQTLPTAPQVANQITV
                     GWNLGNTLEAQCGETAWGNPVVSQQFINAVKAAGFNTIRIPAAWNCHTLPAGSLTIDP
                     AWMARVKQVVDYAYSQNMYVILNIHWDGGWLEEHPLFANQAANNERQAAFWTQIATTF
                     RDYNERMLFAGTNEVHADYGAPTSEHITVQQSYNQTFVNAVRATGGNNASRTLVVQTY
                     NTNLHHGLQYFSLPTDTIASRLMVEIHHYDPYDFTLNPNGSCLYWGAQFPVQSNCTWA
                     NEAYHDLYFGQVGDRWVDQGVPVIIGEYGVATRPNLSLDSRAFWNEYVNRAATANGIK
                     TYYWDNGVLPSATNGFALFNRTTGAVVDQAVLDAVLRGSGVGDPTRTYTLTTAVNGSG
                     TVSRTPTGNPLPGGTSVTLTATPASGYQFAGWTGGASGATNPVTIRMLANTTVTANFI
                     QQGTGGTGQILREYWLNVAGTAVSSLTGSAAYPASPTGSEMLTALEGATNIADNYGAR
                     IRGYVHPLITGQYTFSFASDDAGDLLLSTSDNPANATRIAYVAEWTDPRQWTKFATQK
                     SATINLTAGQKYYIEVLHKEATGGDHFAVAWEGPGIPLTVIGGNLISPFVSGPVTSFN
                     LAVTKNGTGTGTVTSSP"
     gene            2203..>2965
                     /locus_tag="VIV63_08630"
     CDS             2203..>2965
                     /locus_tag="VIV63_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HEY6124702.1"
                     /translation="MKVETGFLTRALALICLTMSAAHGASVHLKYDASSPQASYAARK
                     LSASLSELGHTLPDDPAKSNYRIDLSIRPKRLAAESFSISADANRISIEGGDSRGLIY
                     GALALREQLLNGTPLEKIATARETPALPFRGIKFNTPWDTYRPSSALDQHYATARDLK
                     FWEAWLDMMVENRFNAFTLWTMHPFTYMVRPKNFPEAGKWSDAEFAEWQHLYREIFRM
                     AKERGLDTYIVFWSIFVSEEFAKAHNVAGKNFYPHY"
BASE COUNT          534 a          912 c          930 g          589 t
ORIGIN      
        1 acggcgacga cgtgacggta cccgtgccgg tgccgttctt cgtgaccgcg aggttgaagg
       61 acgtcaccgg ccccgagacg aacggcgaga tgaggtttcc gccgatcacc gtgagcggaa
      121 tgcccggacc ttcccacgcc acggcgaagt ggtcaccgcc cgtggcctct ttgtgcagca
      181 cttcgatgta gtacttctgg cccgcggtca ggttgatcgt cgccgacttc tgcgtggcga
      241 acttggtcca ctggcgcgga tccgtccact ccgccacgta cgcgatgcgc gtggcgttcg
      301 ccggattgtc gctggtgctc agcagcagat cgccggcatc gtcactcgcg aacgagaacg
      361 tgtactggcc cgtgatcagc gggtgcacat acccgcgaat gcgcgcgcca tagttgtcgg
      421 cgatgttggt cgcgccttcg agggccgtga gcatctcgct gccggtcggg ctcgccgggt
      481 acgccgcgct gccggtcaaa ctactcacgg cggtgcccgc gacgttgagc cagtactcac
      541 gcaggatctg acccgtgccg ccggtgccct gctgaatgaa attggccgtc accgtcgtgt
      601 tggcaagcat gcggatggtg acggggttgg tcgccccgct cgcgcccccg gtccagcccg
      661 cgaactggta gcccgaggcc ggggtcgccg tgagggtcac ggaggtaccg cccggcagag
      721 gattgcccgt cggcgtgcgg ctcaccgtgc ccgagccatt cacggcggtg gtgagcgtgt
      781 aagtccgcgt cggatcgccg acgccggagc cccgcagaac ggcgtccagc acggcctggt
      841 cgacgaccgc tcccgtggtg cgattgaaca gcgcgaaccc gttggtcgcg ctcgggagta
      901 cgccgttgtc ccagtagtaa gtcttgatgc cgttcgcggt cgcggcacgg ttcacgtact
      961 cgttccagaa cgcgcgcgag tccaggctga ggtttggccg cgtagccacg ccgtactcgc
     1021 cgatgatgac cgggacgccc tgatccaccc agcggtcacc gacctgcccg aagtacagat
     1081 cgtggtacgc ctcgttggcc caggtgcagt tgctctgcac ggggaactgc gcgccccagt
     1141 acaggcaaga cccgttcggg ttgagcgtga agtcgtacgg atcgtagtga tggatctcga
     1201 ccatcaaccg gctggcgatg gtgtcggtgg gtaacgagaa atattgcagg ccgtggtgaa
     1261 gattcgtgtt gtacgtctgt accacgagcg ttcgcgacgc gttgttgccg ccggtggcac
     1321 gcacggcgtt gacgaacgtc tggttgtacg actgctggac cgtgatgtgt tcgctggtcg
     1381 gcgcgccgta gtccgcatgc acttcgttcg tgcccgcgaa caacatgcgt tcgttgtaat
     1441 ccctgaacgt ggtggcgatc tgcgtccaga aggccgcctg gcgctcgttg ttagccgcct
     1501 gattggcgaa cagcggatgt tcctcgagcc atccgccgtc ccagtggatg ttgaggatga
     1561 cgtacatgtt ctggctgtac gcgtagtcga ccacctgctt gacgcgcgcc atccaggccg
     1621 gatcgatggt cagcgaaccc gcgggcaagg tgtggcaatt ccaggccgct ggaatgcgga
     1681 tggtattgaa ccccgcggct ttcacggcgt tgatgaattg ctggctgacg accggattgc
     1741 cccacgcggt ttcaccgcat tgagcttcga gcgtgttgcc gagattccag ccgaccgtga
     1801 tctggttcgc gacttgcggc gcggtcggaa gggtttgtgc gaacgtggaa atggacgtga
     1861 agagcagcgc gcagaatgcg gcagcaatcc gccatttcgc agcggaaggt ctgcgaagcg
     1921 acatgaagtt tccccctgtt gatcaatcct tgagggtgcg gccgcttgca gcgccgaacc
     1981 tgcgcggatt tatgcactga cgcagtcact aatgcaatgc cgttttaccg gaaaaatctg
     2041 tcagacggtg cgtaacgagg atgctgctct gcgattggcg tattcttccg cagcgttacc
     2101 gctaccttga cctaacaacc cacggggaat cttttttact ctgccaatgt ctggctcgct
     2161 gtcactgcga atgtccagag acttttcatc gaacgagaac ccatgaaagt agaaaccgga
     2221 tttctcactc gcgcgctcgc gctcatctgc ctgacgatga gcgcggccca cggcgccagc
     2281 gttcatctga aatacgacgc ttcctcgccg caggcctcct acgccgcgcg aaagctgtcg
     2341 gcatcgctca gtgagctcgg tcacacgctg cccgacgatc ccgcgaaatc gaactaccgc
     2401 atcgacttgt cgatacgccc gaaacggctg gcggccgagt cgttctcgat atccgcagac
     2461 gccaacagga tttcgatcga gggtggagac agccgtggtc tcatctacgg agcgctcgcg
     2521 cttcgagagc aactcctgaa cggaacgccc cttgaaaaga ttgcgacggc gcgagaaacg
     2581 cctgcgctgc cgtttcgcgg catcaagttc aatacaccct gggacactta ccgccccagc
     2641 tcggcgctcg accagcacta cgccaccgcg cgcgatctca agttctggga agcctggctc
     2701 gacatgatgg tggagaaccg cttcaacgcg ttcacgctct ggaccatgca tccgttcact
     2761 tacatggtgc ggccgaagaa tttccccgaa gccggcaagt ggtcggacgc cgagtttgcc
     2821 gaatggcagc acctgtaccg cgaaatcttc cgcatggcga aggagcgcgg actggatacc
     2881 tacatcgtgt tctggagcat cttcgtgagc gaagagttcg cgaaggcgca caacgtcgcc
     2941 ggcaagaatt tttatccgca ctact
//
LOCUS       DATDEU010000365         4432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1963571, whole genome shotgun sequence.
ACCESSION   DATDEU010000365 DATDEU010000000
VERSION     DATDEU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963571"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VIV63_08635"
     CDS             <1..252
                     /locus_tag="VIV63_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124703.1"
                     /translation="ETATRDLTVSRDKEAGTVTRESNYTTFDGREGSKSDVVQRTDDG
                     YTRDTVRTMPSGETQTRAVDVSCDKDAGKCVKQVEVNKP"
     gene            complement(249..1466)
                     /locus_tag="VIV63_08640"
     CDS             complement(249..1466)
                     /locus_tag="VIV63_08640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6124704.1"
                     /translation="MLFVSDVYFPRVNGVSTSIRTFRADLAQLGVATTLVTPAYPGAR
                     EDSDPSIIRVPSGSVPRDPEDRRFLGGPLRKVLDGALAAKVDLVHIHTPFIAHYAAVR
                     FAREHGLPVVATYHTFFEDYLHHYVPILPRRIGRWIARRFTLSQCADVAELISPSAPM
                     REALQAYGVTTPIEVLPTGLSAESYIRGDGARFRKAFDLPADRPLLLYVGRVAFEKNI
                     DFLLRMFVRLRAHRPDALFVIAGEGPARDHLFRLARELGLESHVRFIGYLDRATELPD
                     CYAAGDAFVFASRTETQGLVLLEAMAQGTPVVSTAELGTRSILTAECGAYVVPENEET
                     FAAAVVGALSLAPDSPRRAQLRAHAESWASLAMARRLQSFYEKVLANKLRSAPEKERG
                     PVGTPLNAASENR"
     gene            complement(1472..2005)
                     /locus_tag="VIV63_08645"
     CDS             complement(1472..2005)
                     /locus_tag="VIV63_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY6124705.1"
                     /translation="MASHGSLLARFDAAEYRLCRRLNRGVENTGIRVFFKTASRLGDG
                     VIWYALMLALPFIYGSQGLKVALIMLASSAVGLAVYKYLKRTFVRERPFIRHAGISLA
                     GAPLDRYSFPSGHTLHAVAFTWQACAAFPELSLVLVPLAMAIAASRVVLGLHYPTDVL
                     VGALLGAASGAIGASFI"
     gene            2163..4229
                     /locus_tag="VIV63_08650"
     CDS             2163..4229
                     /locus_tag="VIV63_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124706.1"
                     /translation="MTYRPLATLLLLLGLAPGARAIDSIVLEVGELRVDGIPVEGASV
                     RLDLLSDTQTRLTLNARAVTLPEPAGKLTNLAWVCTAPVIAEPRFGCDAGRFTGRGGP
                     TGAFDVALNAQMRTDTGVTTFGGRGLNLAGTTATFDGQLDEKGWQVKGKTGSTTVDSL
                     RKFLTPWFALPKDITGDGKVTIEGSAADAGTGTVLDATMKFDAVTLTNEASTIVTDNM
                     GATARLRARLNDADTGLELHVSGTQGQVLVGPVLLDFGKNALALESRAKLAGDLMTIE
                     SLRLTQKDLIDLTGSGRVNLAGEVPSVSGDFHLARLQFPAAYTSYLQITLATTRVLSD
                     LKMGGSLDGDFSVRDNGITSLRISPKDLKVNEESDALHLSNVNGEVNWRPEGVPDAPA
                     SRLSWSRGGAYGLSGGAAAFEFLAQGAGFHVTRPTKLPIFDGALAIDTLALGNLDGEN
                     MEVAFKGRVEPISMPLLTKAFGWPEFSGTLAASIPGVTLKDNVLTFQGNVESQVFGGH
                     VVGSNIRMQDPLGRFPQFFADVRARDLDLGLLTQTFEVGTITGKLEVDVLGLELFAWT
                     PTAFEARLATPKGDKSRRRISAKAVSSLSNVGGGDGGVVQALQSGVLRFFDEYSYEKL
                     GITCKLVGDVCEMSGIEPAGVGYYIVKGAGLPRIDIVGSQGRVNWNQLMSSISTAEFG
                     GATVDP"
BASE COUNT          856 a         1374 c         1481 g          721 t
ORIGIN      
        1 gagacggcca cccgggattt gacggtctcg cgtgacaagg aagcgggtac ggtcacgcgc
       61 gaatcgaact acacgacgtt cgacggccgc gaaggctcga agtcggatgt ggtccagcga
      121 accgatgatg gatacacgcg cgacaccgtg cgcacgatgc cgagcggtga aacgcaaacc
      181 cgcgccgtcg atgtttcctg tgacaaggac gcgggcaagt gcgtcaagca ggtcgaggtc
      241 aacaagccct aacgattttc ggaagctgcg ttcaacgggg tcccgacggg accccgttct
      301 ttttcgggag ctgatcgcag cttgtttgcc aggacttttt cgtagaagga ctgaagtcgc
      361 ctcgccatgg ccagcgacgc ccacgactcg gcatgggccc gcagttgcgc tcgacgcggc
      421 gagtccggcg ccagcgaaag ggctcccacc acggcggccg cgaaggtttc ttcgttttcg
      481 ggcacgacat atgcgccgca ttccgcggtc aggatcgagc gtgtgccgag ctcggccgtg
      541 gacacgaccg gcgtgccctg cgccatggcc tccagcaaca ccaggccctg cgtctcggta
      601 cgcgaagcga acacgaatgc gtcaccggcc gcgtaacaat ccggaagctc ggtggcgcga
      661 tcgagataac cgatgaagcg cacgtgcgat tcgagtccga gctcgcgtgc caggcggaac
      721 agatggtcgc gcgcgggacc ttcgcccgcg atgacgaaca atgcgtcggg ccggtgcgct
      781 cgcagcctga cgaacatgcg cagcaggaag tcgatgttct tctcgaaggc cacacgcccc
      841 acgtagagca gcagcggccg gtcggctggc agatcgaacg ccttgcgaaa gcgcgcgccg
      901 tcgccgcgga tgtaactctc ggcggacagt ccggtgggca acacttcgat cggcgtggtc
      961 acgccgtaag cctgcagcgc ctcgcgcatc ggcgcgctcg gagagatcag ctcggcgaca
     1021 tcggcacact gcgacaacgt gaaacgccgt gcgatccagc ggccgatgcg tcgcggcagg
     1081 atcggcacgt agtgatgcag gtagtcctcg aagaacgtgt gataggtggc caccaccggc
     1141 agaccgtgct cgcgcgcgaa acgcacggcg gcgtagtgcg cgatgaacgg cgtgtggatg
     1201 tgcaccaaat ctactttcgc cgcgagcgcg ccgtcgagaa ccttgcgtag cgggccaccc
     1261 agaaagcgcc ggtcttccgg atcgcgcggc accgaccccg aaggaacgcg gatgatcgag
     1321 gggtcggaat cttcgcgggc gcccggatag gcgggcgtca ccagcgtggt cgcgacaccc
     1381 agttgcgcca ggtcggcacg aaaagtccgg atggaagtgg agacgccgtt gacccgcggg
     1441 aagtagacgt ccgagacgaa cagcacgcgc atcaaatgaa cgaagctccg atggcgcccg
     1501 acgctgcgcc taacaaggcg cccaccagca cgtcggtggg atagtgcaat cccagcacga
     1561 cgcgtgacgc cgcgatcgcc atcgcgagtg gcacgagcac cagtgaaagc tcggggaatg
     1621 ccgcgcaggc ctgccaggtg aatgccaccg catgcagcgt gtggccggac ggaaagctgt
     1681 agcgatcgag gggcgcaccg gccagcgata tgcccgcgtg gcggatgaac ggccgctcac
     1741 gcacgaaggt ccgcttgaga tatttgtaga ccgcgagtcc gacggcgctg ctcgcgagca
     1801 tgatcagcgc gaccttcaga ccctggctgc cgtagatgaa gggcagcgcg agcatgagcg
     1861 cgtaccagat gacgccgtcg ccgaggcggc tcgcggtctt gaagaacacg cggataccgg
     1921 tgttctcgac tccgcggttg agacggcgac acaggcggta ctccgccgcg tcgaatcgag
     1981 ccagtagtga gccatggctt gccatgggtt acgcagtctg ggtttcgaac aaggctgcgc
     2041 gatgacgacg gtgtgaagga tttgttgcca agtcactgaa atccctcagc cgggcggctg
     2101 aattgagagg gcacgaggtc cgacaggagt ggccccctag ggcggggcta caatcgcgac
     2161 ccatgaccta ccggcccctc gcgacactgc ttctgttgtt agggctcgcc cccggggcgc
     2221 gcgccatcga ctcgatcgtg ctcgaggtgg gtgaattgcg tgttgacggc attcccgtcg
     2281 aaggcgccag cgtgcggctc gatctgctct cggatacgca aacgcgcctg acgttgaatg
     2341 cacgcgccgt cacgctgccg gagccagccg gcaaactcac caaccttgcg tgggtctgca
     2401 ctgccccggt gattgccgag ccacgctttg gttgcgacgc cggaagattc accggtcgag
     2461 gcggccccac gggcgctttc gacgtggcgc tgaacgcgca aatgcgcacc gacacgggcg
     2521 tgactacttt cggcggccgc ggcctcaatc ttgcgggaac gacggccacg ttcgacgggc
     2581 aattggatga aaagggttgg caggtcaaag gaaagacggg atcgacgaca gtcgattcgc
     2641 tgcgcaagtt tttgacccca tggtttgcgc tgccgaaaga catcacgggc gacggcaaag
     2701 tgacgatcga aggcagcgct gcggacgcgg ggacgggcac ggtcctggac gccacgatga
     2761 agttcgacgc ggtcacgctg accaacgagg cgagcaccat cgtcaccgac aacatgggcg
     2821 cgaccgcgag actgcgcgcc cgcctgaacg acgccgacac cgggctcgaa ctacacgtga
     2881 gcggtacgca gggacaggtg ctggtgggtc cggtgctgct cgatttcggc aagaacgcgc
     2941 tcgcgctcga gtcgcgggcg aagctcgcgg gcgatctcat gacgatcgag tcgctgcggc
     3001 tcacgcagaa ggatctcatc gacctcacgg gcagcggccg cgtgaatctg gcaggcgagg
     3061 ttccgagcgt cagcggcgac tttcatctcg ccagactcca gttccccgcc gcgtacacga
     3121 gttatctgca gatcacgctg gccaccacgc gcgtgctcag cgacctcaag atgggcggca
     3181 gtctcgatgg cgatttctcg gtgcgcgaca acggcatcac gtcgctgcgc atttctccga
     3241 aagacctgaa ggtcaacgag gagagcgatg ccctgcatct ctccaacgtg aacggcgaag
     3301 tgaactggcg gcccgagggt gtgccggacg cacccgcatc ccgactctcg tggtcgcgcg
     3361 gcggcgcgta tggattgtcc ggcggtgcgg ccgctttcga gttcctggcg cagggcgcgg
     3421 gatttcacgt gacgcgcccc accaaactac ccattttcga cggcgcactc gccatagaca
     3481 ccctggcgct cggcaatctc gacggcgaga acatggaggt cgcgttcaag ggcagggtcg
     3541 agcccatcag catgccgctg ctgaccaagg cgttcggctg gccggagttc tcgggaacgc
     3601 tcgccgccag cattcccggc gtcacgctga aggacaacgt gctcacgttc cagggtaacg
     3661 tcgagtcgca ggtgttcggc ggtcacgtgg tcggcagcaa catccgcatg caggatccgc
     3721 tcggacgatt cccgcagttc ttcgccgacg tgcgcgcgcg cgatctcgat ctcggtctgc
     3781 tcacgcagac tttcgaagtc ggcaccatca ccggcaaact cgaggtcgac gttctcggtc
     3841 tcgagttgtt cgcatggact cccaccgcat tcgaagcgcg gcttgccacg ccgaagggcg
     3901 acaaatcacg acgccgcatc agcgccaagg ccgtgagcag cctgtcgaac gtgggcggcg
     3961 gtgatggcgg cgtggtgcag gcgctgcaat cgggcgtgct gcgattcttc gacgagtaca
     4021 gctacgagaa gctgggcatc acctgcaaac tcgtcggcga cgtgtgcgag atgtcgggaa
     4081 tcgaaccggc gggagtcggc tactacatcg tcaagggggc gggactgccg cgcatcgata
     4141 tcgtcggcag ccaggggcgc gtgaactgga atcagctgat gtcgtcgatc tcgaccgcgg
     4201 agttcggcgg tgcgacggtg gacccgtgag aggtcctcga tggactccgc gcccattcgg
     4261 gccaataatc gcgcgcacat ttgacgtaag gcacgttcag ggcgcattca tcgatcaagc
     4321 ccgatcatgt ccgcgtccag gcctgcgctt ggaataagga gttatcaatg cgacgcgtat
     4381 tgccgctgaa gattgcactg gcgagtttgc tgatcgccgc gtgcgtcacc at
//
LOCUS       DATDEU010000366         4296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_358113, whole genome shotgun sequence.
ACCESSION   DATDEU010000366 DATDEU010000000
VERSION     DATDEU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358113"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..514)
                     /locus_tag="VIV63_08655"
     CDS             complement(89..514)
                     /locus_tag="VIV63_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY6124707.1"
                     /translation="MDISINLTDGVPIYRQIFNQVKYLVASGLLQPGEELPPIRTLAL
                     QLKVTPNTIVKAYGELESSGVVQKRQGSGTFVSEGRPQQVALRERRRVIEQRIDALLA
                     EAHQLNFTTDDILRMVRERKAAMDETTVEPAVVKKEANR"
     gene            complement(981..1916)
                     /locus_tag="VIV63_08660"
     CDS             complement(981..1916)
                     /locus_tag="VIV63_08660"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY6124708.1"
                     /translation="MKTRAFIALSILLGSPAFLHGDAQAAQRDSARAAIEAQSASFTA
                     SLAKGDATEAAQIFTVNAMLSVPGVEGVLEGRQAIEKFWQAALAGGMKSLTLTPREVD
                     GSGDLRIETGTYAAFGANRSELGRGEYLMVWKREEGAWKIHRDYGHASGAMPVANAIA
                     EDRVGLPRDYATAMRQVSDTVYDEKSGMTTVFANDLAASIAGFSQQHYPDGAVILMEF
                     AQPQRDGEGELLRDARGAPIKGPIDHIDVMRRGPDFGASYGAERAGEWEFASYRSDGS
                     VRVASANAVNCAACHRNAGADKDFVFRTRPWLPLK"
     gene            complement(2025..2789)
                     /locus_tag="VIV63_08665"
     CDS             complement(2025..2789)
                     /locus_tag="VIV63_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124709.1"
                     /translation="MTFRVLIVDDEPLARQRLQRILRGEKDIELLPPCENGRAAVAAI
                     LEQQPDLLFLDVQMPGMSGFDVLAAVGAKQMPAVIFVTAHDRFALQAFEAEALDYLLK
                     PFGAERVQKSLERARDFLAGGARRKLERQYAGLLRATSGVRETPCILVKKRERIIVLH
                     PEEIDFVEAYGDYVRLRAGTEAHLLRGTLADMERRLKPEGFVRIHRSRLVNWRRVREF
                     IADRDHDPVVVLHSGQRLEASPAHLKDLQRRFFGKG"
     gene            complement(2786..3877)
                     /locus_tag="VIV63_08670"
     CDS             complement(2786..3877)
                     /locus_tag="VIV63_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6124710.1"
                     /translation="MSSTPRKFSFWPRWLVYAGLWLLLTCIFASQLYFAGYVTPWRNA
                     FVLEAVYWLSWWILSPLVFWWCRRLRDAGWYLRAPALLLGALVALFLAPLIALLLELT
                     LSWLRLCLGECQQAVALFSAEWFRLAVRVAGVNLPVYAAFVLAWHAATFYRDARDRQM
                     RAVELESLLQQAQLEALRSQLNPHFLFNALHSMAELVHTDPKLAEQLLVRLGELLRQV
                     LQSSTRPEITLAEELEFIRGYVEIEQMRLGERLQVRWDIDAGALEAHVPSLILQPIVE
                     NAVQHGVAAATGAGTLLIAARRDGNFLQLEIGDNGPGLAQTSVDRRQGIGLANTRARL
                     QRLYGDRQTFELQAGDGLTVRIRIPVSAT"
     gene            4050..>4296
                     /locus_tag="VIV63_08675"
     CDS             4050..>4296
                     /locus_tag="VIV63_08675"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6124711.1"
                     /translation="MDIVELLADAEEGLTVSEISQRLKRRMSELFRIIVVMERRGWLQ
                     KDPETSRYSMSYHMLRLAHRGTPTQTLTSAAAPAMQEL"
BASE COUNT          790 a         1406 c         1308 g          792 t
ORIGIN      
        1 gtgcgatctt gcccccgaac gaacgcgaga ggccgttgac gtcgacaatt gactgtccca
       61 tatttttctc cctgcttcct gctcttgcct atctattcgc ttctttcttc accaccgccg
      121 gctcgacggt ggtctcgtcc atcgccgcct tgcgctcccg gaccattcgc agaatgtcgt
      181 ccgtggtgaa gttcaactgg tgcgcctcgg ccagcaacgc gtcgatgcgc tgctcgatta
      241 cgcggcgccg ttcccggagc gccacttgct gcggacgccc ttccgagacg aacgtgcccg
      301 acccctgccg cttctgaacg actcccgagg attccagttc tccataggcc ttcacgatgg
      361 tgttcggcgt gaccttgagc tgcagcgcca gcgtccgtat cgggggtagc tcttcaccgg
      421 gctgaagcag gccggacgcc accaagtatt tgacttgatt gaagatctgg cggtagatcg
      481 gcacgccgtc ggtcaggttg atggagatat ccaaggcggt cgcttcggcc cgtgtcagtt
      541 caagtgtatg ggtacactga tacaattttc ggagaccgtc aagcgccgcg tgcggatgtc
      601 cggcgcgggg gacaaactac tcaccggagg attgggcgca tgggttagcg ccactttgtt
      661 gccaccattc cccgccagcc tccttcactg aacgaaggtc ttctgtcagg agtggcgacg
      721 atggagcaga tcgagaaccg gattgacctt caccgcagct cagttatcgg cgcgatcacc
      781 gcagtccact tcgtactttc cgtcgcgatg ttcttcctga gcatggcgag cgggatgtcg
      841 cggttcgatg gcgcaggacc gtcggatctc gcgcatctct tgctgacggg tgcgttcacg
      901 atactgagtt ttccactgct ccccgcggtt caggggctgg gttggctgga gcgcgatccg
      961 gttctggcga tcccggcaac ttacttgagc ggtagccagg gccgcgtgcg gaaaacgaaa
     1021 tctttgtcag cgcctgcgtt gcggtggcag gcagcgcagt tcacggcatt ggcggaagcg
     1081 acccgtacgc tgccatcgga gcgataactg gcgaactccc attccccggc gcgctccgct
     1141 ccgtatgacg caccgaagtc cggccctcgc cgcatcacgt cgatgtgatc tatcggcccc
     1201 ttgataggcg cgcctcgcgc atcgcgcaac agctcaccct ctccatcgcg ctgtggctgg
     1261 gcgaattcca tgagaatcac agcgccatcc ggataatgct gttgagagaa tccggcgatc
     1321 gaagccgcca gatcgttggc gaacactgtg gtcatgccgg acttctcgtc gtagaccgtg
     1381 tcactgacct ggcgcatcgc cgtcgcatag tcgcgcggca ggcccacacg gtcttcggct
     1441 attgcgttcg cgacgggcat tgcgccgctg gcatgaccat agtcgcgatg aatcttccac
     1501 gcgccctctt cgcgtttcca caccatcagg tattccccgc gccccaactc gctgcggttg
     1561 gcgccaaacg ccgcgtaggt gccggtttcg atgcgcaggt caccggagcc atcgacctcc
     1621 cgcggcgtca gcgtcagcga cttcatgccg ccagccagcg cggcctgcca gaatttttcg
     1681 atagcctggc gtccttcgag cacgccttcg acgccgggaa cgctgagcat cgcgttcacg
     1741 gtgaaaatct gcgcagcttc ggtggcgtcg cccttcgcaa gtgacgccgt gaacgaggcg
     1801 gattgcgcct cgatggcggc gcgtgcggaa tcacgctgcg cggcctgtgc gtccccgtga
     1861 aggaatgcag gtgagcccag cagtatcgat agagcgatga aggcgcgtgt tttcatggcg
     1921 cgcattcagc gcgcgccacg gggactttga aaacggtttg cgaccagctc gacgcggggc
     1981 acgacgaagg ccgcgattcg cgcgacgaag gtccggcgcg ccgctcagcc tttcccgaaa
     2041 aagcgccgct gcaaatcctt cagatgtgcc gggctcgcct cgagccgttg gccgctgtgc
     2101 agcaccacca cgggatcgtg atcgcgatcc gcgatgaact cgcgtacgcg ccgccagttc
     2161 accagccgtg accggtggat tctcacgaaa ccctcaggct tcaaccgtcg ctccatatcg
     2221 gccagcgtgc cacggagcag atgcgcctcg gttcccgcgc gcaatcgtac gtagtcacca
     2281 taggcctcga cgaaatcgat ttcttcggga tgcaggacga tgatgcgctc gcgtttcttc
     2341 acgaggatgc agggcgtttc gcgcaccccg gacgtggcgc gcaacagccc ggcgtattgc
     2401 cgttcgagtt tgcgacgcgc accgcccgcg aggaaatcgc gggcgcgttc cagtgacttc
     2461 tgcacgcgtt cggcaccgaa gggcttgagc agatagtcga gggcttcggc ttcgaaagcc
     2521 tgcaaggcga agcgatcgtg tgccgtgacg aaaatcacag cgggcatttg tttcgcaccg
     2581 acagccgcga gcacgtcgaa tccgctcatg cccggcatct gcacatcgag gaacaggaga
     2641 tccggctgct gctcgaggat tgccgccacc gcggcccggc cattctcgca gggcggcagt
     2701 agttcgatat ccttttcgcc gcgcaggatg cgctgcagtc gctggcgcgc caacggctcg
     2761 tcgtccacga tgagcactcg gaaggtcatg tcgccgacac cgggatgcgt attcgcacgg
     2821 tgaggccatc gcctgcctgc aactcgaaag tctgacgatc gccatacagc cgctgcagtc
     2881 gcgcgcgcgt gttcgcgagt ccgattccct gtcgccgatc caccgaggtt tgagccaacc
     2941 cggggccgtt gtcgccgatc tcgagctgca ggaaatttcc atcgcgacgg gcggcaatga
     3001 gcagagtgcc agcacccgtg gccgcggcca cgccgtgttg cacggcgttt tccacgattg
     3061 gctgcaggat gaggctgggc acgtgcgcct cgagcgcccc cgcgtcgatg tcccagcgaa
     3121 cttgcaaccg ctcacccagt cgcatctgct cgatctcgac atagccgcga atgaactcca
     3181 actcctcggc cagcgtgatt tccgggcgcg tcgacgactg caatacctgc ctcagcaact
     3241 cgccgagacg cacgagcagc tgttccgcga gtttcggatc ggtgtgcacc agctcagcca
     3301 ttgaatgcag cgcgttgaag agaaagtgcg gattgagctg actgcgcagt gcctcgagct
     3361 gcgcctgttg cagcagcgat tccagctcca ccgccctcat ctgccggtct cgcgcgtcgc
     3421 ggtagaacgt ggcggcatgc caggcgagca cgaaagcggc atacaccggc agattcacgc
     3481 cggcgacgcg gacggccagg cgaaaccact ccgccgaaaa gagcgcgacg gcctgctggc
     3541 attcgcccaa gcacagacgc agccacgaaa gcgtgagctc cagcagtagt gcaatcagcg
     3601 gcgcgagaaa gagcgcgacc aatgcgccca gcaagagcgc cggcgcacgc aggtaccaac
     3661 ccgcgtcgcg caagcgccgg caccaccaga acacaagtgg ggacaggatc caccatgaga
     3721 gccagtacac ggcttcgagt acaaaagcgt tccgccacgg cgtgacgtag cccgcgaagt
     3781 agagctggga cgcgaaaatg caggtcagca gcaaccacag gccggcgtag accagccatc
     3841 gcggccagaa gctgaatttg cgcggcgttg agctcacgcg cgcactttta gcagaaagcg
     3901 ggcctccgtt cccgggctcc gcgccgcccg aggaatgtcg attctcgcct cccgggcatg
     3961 taatctcccc ccgaatgccg cgctcaaaac cccgttcagg caaatccata cccgtccgcg
     4021 gctattcagc tcccgcgctc gagaagggca tggacatcgt cgaattgctc gccgacgcgg
     4081 aagaaggcct caccgtgtcg gagatttctc agcggctcaa gcggcgcatg agcgaactgt
     4141 tccgcatcat cgtggtgatg gaacgccggg gctggctgca gaaagacccg gagacttcgc
     4201 gttacagcat gtcgtatcac atgctgcggc tcgcgcatcg cggcacgccc acgcagaccc
     4261 tgacctccgc cgcggcgccc gcgatgcagg agctat
//
LOCUS       DATDEU010000367        10553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_797871, whole genome shotgun sequence.
ACCESSION   DATDEU010000367 DATDEU010000000
VERSION     DATDEU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797871"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VIV63_08680"
     CDS             complement(<1..1244)
                     /locus_tag="VIV63_08680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6124712.1"
                     /translation="MNASGPAQRDVMEYDVAVVGGGPAGLSAAIRLKQLRPELTVCIL
                     EKGSTIGAHSLSGAVMEPGPLDALLPEWRTAPLPIRVPVTRDEFRLLGKNGSRRLPWF
                     PQTLHNTGNFIVSLGGLNQWLATQAEQLGVDVFAGFAAALPLFGDDGSVSGVQIGDMG
                     VQHDGSRGPNYTAGPEVHARLTIIAEGCRGSLAKQLIKRYKLDAGCDPQVYALGMKEL
                     WQLPAGRVQPGLVQHSLGWPLDSRTYGGSFVYHLNDNRAYVGFVTGLDYVDPRFSPFE
                     AFQQFKHHPEMHAFLQGGEILSAGARSISAGGWQSMPKLEMPGAVLIGDSAGTLNVPK
                     IKGIHQAIRCGMAAADYFAATDTSIGFDAEWRKSAAVAELREVRNVKPGFKRGMWLGM
                     INAAIESVFGGRTPWTLRNTAS"
     gene            complement(1247..2014)
                     /locus_tag="VIV63_08685"
     CDS             complement(1247..2014)
                     /locus_tag="VIV63_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6124713.1"
                     /translation="MKYADALAVVTGGASGLGNAVARHVVAAGGRVTLLDVQEGPGQA
                     AASALGPNAFFAKCDVTSEAEVNAAMESARAAMGGINLLVNCAGVIGAGRVLGKNGPM
                     AGDFFTRVVHINLIGTFLCDKAAAAIMQSNAPSADGERGVIVHTSSVAAFEGQIGQAA
                     YSATKAAVAGMTLPIARELAMFGIRVCSLAPGIFGTPMLGAMPPEVQESLGKQVPFPQ
                     RLGKPEEFAALVQTIFEVPYLNGETIRLDGAIRMQPK"
     gene            complement(2074..3255)
                     /locus_tag="VIV63_08690"
     CDS             complement(2074..3255)
                     /locus_tag="VIV63_08690"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY6124714.1"
                     /translation="MDNRQVIIAAATRTPIGAFQGVLAPVTAPQLGAAAIKGALAAAG
                     IAGADIQEVIMGCVLPAGLGQAPARQAALGAGIPTGVPTTTINKMCGSGMKAIMMAAD
                     QIRAGDIDRAVAGGLESMTNAPYLLPKARGGMRMGHGEVLDHMFYDGLQSPFDGQLMG
                     VFAENTAQKYGFSRADQDAFATESTQRALKAVNSGAFANEIAPVTVKSRKGETVVDKD
                     ETPFTVDLAKITTLKPAFRKDGTVTAASSSSISDGAAAAVLMSAAEAERRGVKPLARI
                     LGYTSFAHEPEWFTTAPAGAIQKLLTKLSWKAGDVHLYEVNEAFAAVTMAAMRDLGFD
                     HSRTNINGGACALGHPVGATGARIIATLVHALRARGGGRGIASLCIGGGEATAIAVEV
                     P"
     gene            complement(3267..3926)
                     /locus_tag="VIV63_08695"
     CDS             complement(3267..3926)
                     /locus_tag="VIV63_08695"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6124715.1"
                     /translation="MPAAARKAGTRKDLAAAPSPWESRGRRSAGRALKRDAVILAAAR
                     AFRERGYHNTSLDDLAASLKVTKPTLYLYVPNKEAILFECFRAGLAQIQATLKECESA
                     RGPARERLFAFIRGYAAAIVGDFGWCMLRAEDQHLGAAMSRRIKLLKAGIDKRMRALI
                     DAGVADGSIRACDTRMTAFALAGALNWMGHWYREDASLRPAEIADRFIDVFNRGLRGR
                     D"
     gene            3979..7410
                     /locus_tag="VIV63_08700"
     CDS             3979..7410
                     /locus_tag="VIV63_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY6124716.1"
                     /translation="MKPPEPGRPLRFVSAASLFDGHDAAINMIRRLLQSGGAEVVHLG
                     HNRSVADIVRAAIAEDADAIAVSSYQGGHNEYFRYMIDMLRELNADHIRVVVGGGGTI
                     SPDEIAALESYGVSKIYTPEHGRSLGLNGMIADVFSRVGRRSTGDAGARMPAVHDHGS
                     IAQAITLLEGGDGADDLRRLLSAGIANPQRAPVIGLTGTGGAGKSSLTDELLQRFLRQ
                     FPERNVAVVAVDPTRRRSGGALLGDRIRMNSLAAPQVFMRSLATRRQHLATSAVLADV
                     IALYRAVGFDLIVVETAGIGQSDTEIVDVADLSLYVMTAEYGAASQLEKIDMLDFADM
                     IVLNKFEKRGAEDALRDVRKQWRRNHPDRMKLPDEELPVFPTIASRFNDPGVNRLFLS
                     LCRALDEECGEPGHWTQREGAPLAVAPTEFISRDPLVPSARSRYLAEIAVRGREERSV
                     AARRADAARRAHGLYLSLQALGDPSLPEPMAPAPAADGKNAALDALRAAYNTALHDIG
                     TDALTNLRAWPAREQGVTAPEYSYTVRGREVRGANYSESQSHTQVPKLAAPNLSGWGE
                     ILDFLQNENLPGAYPYTGGVYPYRREEEDPTRMFAGEGAPERTNRRFHYLAAGHEATR
                     LSTAFDSTTLYGEDPDTRPDIFGRTGNSGVSIASLDDMKKLYSGFDLCAPSTSVSMTI
                     NGPAPMILAMYMNTAIDQHVEKFLRGANRWDSAQAALKASGAGSASWPAYRGELPPDH
                     GGEGLGLLGVGGRELVALGVLTDAEYSRLRAEALSRVRGTVQADILKEDQAQNTCIFS
                     TEFALRMMGDVQQFFSDNAVKNFYSVSISGYHIAEAGANPITQLAFTLANGFTIVEYY
                     LARGMKIDDFAPNLSFFFSNGMDAEYAVIGRVARRIWARAMRDVYKAGPRSQMLKYHI
                     QTSGRSLHAREIQFNDIRTTLQAMYALFDNCNSLHTNAYDEALTTPTEESVRRAVAIQ
                     LIINRELGLNRIQNPWSGSYAITSLTDVVEEAVYKEFESLAERGGVLGAMETMYQRGK
                     IQEESMLYETRKHDGTLPIVGVNTFLSEHGEGKQDVAPLFRASDEEQRAQVDAVHAFH
                     ERHSRSAPEALRRLQSVAASGGNVFAELLETVQVCSLGQITRALYEVGGQYRRHM"
     gene            complement(7417..8064)
                     /locus_tag="VIV63_08705"
     CDS             complement(7417..8064)
                     /locus_tag="VIV63_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124717.1"
                     /translation="MELDELKSSWQLDRRVDERILHRLLTDSISRKARWRLAPVLAGA
                     VMNMIIGAFFALVWGKFWSAHLTTPAVAIAGIALHLASVGLIVIGVVRLVLVLRINYT
                     QPVLTIQRSLATLQEFEARSFHAVWFACWVLVPAAMVAIVMGFAGVDLWERASGFILA
                     NFVVCLAGGLAPPLLHYWARRHPGRLAAWMDAFLLSHSIARAKAAVAEIDEFARS"
     gene            complement(8051..8560)
                     /locus_tag="VIV63_08710"
     CDS             complement(8051..8560)
                     /locus_tag="VIV63_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6124718.1"
                     /translation="MNAESGAEFAALLDGHRRLIFKVAHAFAREAADVEDLAQEIAAQ
                     LWRAFPTYDHARLFSTWMYRIALNVAISWLRSEAPRRRRSVAYDADMHEPAVEDAADN
                     DEGQRILRGFIASQTALDRAVLLLYLEETPYSGMSEVLGITVTNLTTKINRLKDRLRT
                     YAEKHHGTR"
     gene            complement(8631..9401)
                     /locus_tag="VIV63_08715"
     CDS             complement(8631..9401)
                     /locus_tag="VIV63_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6124719.1"
                     /translation="MTSTSPPARSPWPAVISVPLRFIYGIYAALLFLVVVVTALLGVL
                     LMPTLELRRGTARIAARSFFLLAGMPLRLRGTENLPAGQCVVVANHASYLDGVVMTAA
                     LPPRFGFVIKREMNDVPVGGLLLRRIGSEFVDRFNRHKGGTDARRVLRTAASGHSLVF
                     FPEGTFTSEVGLGKFHTGAFAIAARAACPVVPAVILGTRRNMPATRVLPRPGPLEVRF
                     GLPIAPTVEGPEVDPALKLRDQSRQAILRELGEPDLAA"
     gene            9562..10494
                     /gene="xerD"
                     /locus_tag="VIV63_08720"
     CDS             9562..10494
                     /gene="xerD"
                     /locus_tag="VIV63_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY6124720.1"
                     /translation="MVNQAKAQNEAPAADTADATVSKFLDAVWMERGLSANTLAAYRA
                     DLTALARWLAERGTPIIKTTRADLLGFIAYRVEAGARPRSTARQLSSFRRFFRYFVRE
                     GVIRDDPTAQIAMPKIGRSLPKSLTEEEVESLLNAPAIADPLGHRDRTMLEVLYATGL
                     RVSELVNLKYNQINTNQGVIRIMGKGNRERLIPLGEEAVRWLTDFVRAPRSEILLDRT
                     TDYLFPTRRGDRMTRQAFWHIIKRYARKAVIDKELSPHTLRHAFATHLLNHGADLRVV
                     QMLLGHSDLSTTQIYTHVARERMKEMHGTHHPRG"
BASE COUNT         1837 a         3430 c         3493 g         1793 t
ORIGIN      
        1 tagctcgcgg tgttgcgcag cgtccagggc gtgcgtccac cgaacacgct ttcgatggcg
       61 gcgttgatca tccccaacca catgccgcgc ttgaagccgg gcttgacgtt gcgcacttcg
      121 cgaagctcgg ccaccgccgc cgacttccgc cattccgcgt cgaagccgat gctggtgtcg
      181 gtggcggcga aatagtccgc cgcggccatg ccacagcgga ttgcctgatg gatgcccttg
      241 atcttcggca cgttgagcgt gcccgcggaa tcgccgatga gcacggcgcc cggcatctcg
      301 agcttcggca tggactgcca gccgcccgcg gagatgctgc gcgcgcccgc cgagagaatt
      361 tcgccaccct gcaggaacgc gtgcatctcg gggtgatgct tgaactgctg gaaagcttcg
      421 aagggactga agcgcggatc gacgtagtcg agtccggtca cgaagccgac gtaggcgcga
      481 ttgtcgttga ggtggtagac gaaactgccg ccgtaggtgc gggagtcgag cggccagccc
      541 aggctgtgtt gcacgaggcc gggctgcaca cggccggccg gcagttgcca cagttctttc
      601 atgccgagcg cgtagacctg cggatcgcag ccggcgtcga gtttgtaacg cttgatgagc
      661 tgcttggcga ggctgccccg gcagccttcg gcgatgatgg tcagccgcgc gtgcacttcc
      721 gggccggcgg tgtagttagg gccgcggctg ccgtcgtgtt gcacgcccat gtcgccgatc
      781 tgcacgccgg aaaccgagcc gtcgtcgccg aacaagggca gcgcggccgc gaagcccgcg
      841 aacacgtcga cgcccaattg ttcggcctgc gtggcgagcc actggttgag gccgcccagc
      901 gagacgatga aattgccggt gttgtgcagg gtctgcggaa accacggaag gcggcggctg
      961 ccgttcttgc cgagcagccg gaattcatcg cgagtgaccg gcacgcggat gggcagcggc
     1021 gcggtgcgcc attccggcag cagcgcgtcg agcggacccg gttccatgac cgcgcccgac
     1081 agggaatgcg cgccgatggt cgagcctttc tcgaggatgc agaccgtgag ctcggggcga
     1141 agttgtttga ggcggatcgc cgccgacagg cccgccggtc cgccgccgac cacggccacg
     1201 tcgtactcca tgacatcgcg ttgtgcgggt cccgaggcgt tcatgtctat ttcggctgca
     1261 tgcggatggc gccgtcgaga cggatggttt cgccattgag atagggcact tcgaaaatgg
     1321 tctgcaccag cgccgcgaat tcttcgggtt tgccgaggcg ctgcgggaac ggcacctgct
     1381 tgccgagcga ttcctgaact tccggcggca tcgcgcccag catgggcgtg ccgaatattc
     1441 cgggcgccag cgagcagacg cggattccga acatggcgag ctcgcgcgcg atgggcagcg
     1501 tcatgccagc cacggcggcc ttggtggccg aataggcggc ctggccgatc tgtccttcga
     1561 aggccgctac agaagaggtg tggacgatga cgccgcgctc gccatccgcc gaaggcgcat
     1621 tcgactgcat gatggcggcg gccgccttgt cgcagaggaa cgtgccgatc aggttgatgt
     1681 gaacgacgcg cgtgaagaaa tcaccggcca tcggtccgtt cttgccgagc acgcggcccg
     1741 cgccgatgac gcccgcgcaa ttcaccagca ggttgattcc gcccatggcg gctcgcgccg
     1801 actccatcgc cgcattgacc tcggcttccg aggtgacgtc gcatttcgcg aagaaagcat
     1861 tgggtccgag cgctgacgct gctgcctgcc ccggaccttc ctgcacgtct aacaacgtga
     1921 cgcggccgcc ggcggccacg acgtgccgcg ccaccgcgtt gccgagaccc gaagcgccac
     1981 ccgtgaccac ggcaagcgcg tctgcgtatt tcatcggaat tcctgtttct caagacctgt
     2041 gcaaagcgcg cacagcatat cccagcgcgc gattcagggg acctcgaccg cgatggcggt
     2101 cgcttcgccg ccgccgatac ataacgaagc tatgccgcga ccaccgccgc gggctcgcaa
     2161 cgcgtgcacc agggtggcga tgatgcgcgc gccggtggcg cccaccggat ggccgagcgc
     2221 acaggcgccg ccattgatgt tggtacgtga gtgatcgaag cccaggtcgc gcatggcggc
     2281 catcgtcacc gcggcgaacg cttcgttcac ctcatacaag tgaacgtcgc ccgccttcca
     2341 cgagagtttc gtgagcagtt tctggatggc gcccgccggt gcggtcgtga accactccgg
     2401 ctcgtgcgcg aagctcgtgt agccgaggat gcgcgccagt ggcttcacgc cgcggcgttc
     2461 ggcttcggca gccgacatca atacagcggc cgcggcgcca tcggagatcg aggacgacga
     2521 ggcggccgtg acggtgccgt ccttgcggaa cgcaggcttg agcgtggtga ttttcgcgag
     2581 atcgacggtg aacggagtct cgtccttgtc gaccacggtc tcgcccttgc ggctcttgac
     2641 cgtgaccggc gcgatctcgt tcgcgaacgc gccggagttg accgccttca aggcgcgctg
     2701 cgtggactcg gtggcgaacg catcctggtc ggcgcgcgag aagccgtact tctgcgccgt
     2761 attctcggcg aatacaccca tgagctggcc gtcgaaaggg ctctgcaggc cgtcgtagaa
     2821 catgtggtcg agcacttcac catgacccat tcgcatgccg ccgcgcgcct tcggcagtag
     2881 atagggtgca ttggtcatgg actcgagtcc gccggccacc gcgcgatcga tgtcgccggc
     2941 gcgaatctgg tccgcggcca tcatgatggc cttcatgccc gagccgcaca tcttgttgat
     3001 ggtggtggtc ggaacgccgg tgggaatgcc tgcgccgagc gcggcctggc gtgcgggcgc
     3061 ctgcccgagg cccgcgggca gcacgcaacc catgatgact tcctggatgt cggcgccggc
     3121 gattcccgcg gcggcaagcg cgcccttgat ggcggcggcg ccgagctgcg gcgcggtgac
     3181 cggcgcgagc acgccctgga aggcgccgat gggcgtacgc gtggcggcgg cgatgatgac
     3241 ttgtctgttg tccatgaaac tctccgttaa tcgcgaccgc gcaaaccgcg gttgaaaacg
     3301 tctatgaaac gatcggcaat ctcggcgggc cggagcgacg cgtcttcgcg ataccagtgg
     3361 cccatccagt tgagggcgcc ggccagcgca aaggcggtca tgcgcgtgtc gcaggcgcgg
     3421 atcgatccgt cggccacgcc ggcgtcgatg agcgcgcgca tgcgcttgtc gatgcctgcc
     3481 ttcagtagtt tgatgcgccg gctcatcgcg gccccgaggt gctggtcttc ggcgcgcagc
     3541 atgcaccagc cgaagtcccc gacgatcgcc gcggcataac cgcggatgaa ggcgaacagc
     3601 ctctcacgcg ccgggccccg ggcgctctcg cattccttga gggtcgcctg gatctgcgcc
     3661 aggccggcgc ggaaacactc gaacaggatg gcttccttgt tcggcacata taaatagagt
     3721 gtgggcttgg tgaccttgag gctggcggcc aggtcgtcga gcgaggtgtt gtgatagccg
     3781 cgttcgcgga atgcccgcgc cgcagcaagg atgaccgcat cgcgcttgag cgcacggccg
     3841 gcgctgcgcc gcccgcgcga ctcccagggg gagggcgcgg cggccaggtc cttgcgggtt
     3901 ccggcttttc tggcggcggc gggcatcggg cggggtgctc gtgatactct gcggtaaccc
     3961 atcttaccag tcagtaacat gaagccacca gagccaggca ggccgctgcg attcgtcagc
     4021 gcggcgtcgc tgttcgacgg ccacgatgcc gccatcaaca tgatccggcg cctgctccag
     4081 tccggtggcg ccgaggtggt gcacctcggg cacaaccgca gcgtcgccga catcgtgcgc
     4141 gccgccatcg ccgaagatgc cgacgccatc gccgtcagca gctatcaggg cggtcacaac
     4201 gaatacttcc gctacatgat cgacatgctc cgcgagctga acgccgacca catccgcgtg
     4261 gtggtgggcg gcggcggcac catctcgccc gatgagatcg ccgcgctcga gtcatatggc
     4321 gtgtccaaga tctacacccc cgaacacggg cgcagcctgg ggttgaacgg catgatcgcc
     4381 gacgtgttct cgcgcgtcgg acggcgctcg accggcgatg cgggcgcgcg tatgcccgcg
     4441 gttcatgatc acggctccat tgcgcaagcc atcacgctgc tggaaggcgg cgacggcgcc
     4501 gacgaccttc ggcgtttgct cagcgccgga atcgccaatc cccagcgtgc gccggtcatt
     4561 ggtctcacgg gcaccggcgg cgccggcaag agttcgctca ccgacgagct gctgcagaga
     4621 ttcctgcggc agtttcccga gcgcaatgtg gcggtcgtgg cggtcgatcc gacgcggcgc
     4681 cgttccggcg gtgcgttgct gggcgaccgc atccgcatga actcgctggc cgcgccccag
     4741 gtgttcatgc gttccctcgc cacgcggcgt cagcatctcg ccaccagcgc cgtgctggcg
     4801 gacgtgatcg cgctgtatcg cgccgtgggt ttcgatctca tcgtggtgga gaccgcgggc
     4861 atcggccaga gcgacacgga aatcgtcgac gtcgcggatc tgtcgctgta cgtgatgacc
     4921 gccgaatacg gcgccgccag ccagctcgag aagatcgaca tgctcgactt cgccgacatg
     4981 atcgtgttga acaagttcga aaagcgcggc gccgaagatg ccctgcgcga cgtgcgcaag
     5041 cagtggcgcc gcaatcatcc cgaccgcatg aagctgcccg acgaagagct gccggtgttt
     5101 cccacgatcg ccagccgctt caacgatccg ggggtgaacc gcctgttcct gtcgctgtgc
     5161 cgcgcgctcg acgaagaatg cggcgagccg ggtcactgga cacagcgcga gggtgcgccg
     5221 ctcgccgtgg cgcccaccga gttcatttca cgcgatccgc tggttccctc ggcgcggtcg
     5281 cgttatctcg cggagattgc cgtacgcggg cgcgaagaac gaagtgttgc cgcgcgccgt
     5341 gcggatgcgg cgcgccgggc tcatggtctc tatctatcgc tgcaggcgct gggcgatccg
     5401 tcgctgcccg agcccatggc tcccgccccg gccgccgatg gcaagaacgc tgcgctcgac
     5461 gcgctgcgcg cggcgtacaa caccgcgctc catgatatcg gcaccgacgc gttgacgaac
     5521 ttgcgcgcat ggcccgcgcg cgagcagggc gtgaccgcgc ccgagtattc gtacaccgtg
     5581 cgcggtcgcg aagtgcgcgg cgcgaactac tctgaatcgc agagccacac gcaggtcccg
     5641 aaactcgccg cgcccaatct gtcgggctgg ggcgagatcc tcgatttcct gcagaacgaa
     5701 aatctgccgg gtgcgtatcc ctacaccggc ggcgtgtatc cctatcgccg tgaagaagag
     5761 gatcccacgc gcatgttcgc cggcgagggc gcgccggagc gcacgaaccg ccgcttccat
     5821 tacctcgccg cggggcacga ggccacgcgc ctctccaccg cgttcgattc caccacgttg
     5881 tacggcgaag accccgacac gcggccggac atcttcggtc gcaccggaaa ctcgggcgtg
     5941 tccattgcct cgctcgatga catgaagaag ctgtattcgg gcttcgatct ctgcgcgccc
     6001 tcgacgtcgg tgtccatgac catcaacggg cctgcgccca tgatcctggc catgtacatg
     6061 aacacggcca tcgatcagca cgtggagaaa ttcctgcgcg gggccaatcg gtgggattcc
     6121 gcccaagcgg cgttgaaagc ctctggcgcg ggctcagcat cctggccggc ctatcgcggc
     6181 gaactgccgc cggaccacgg cggagaaggc ctgggattgt taggcgtggg tgggcgtgaa
     6241 ctcgtggcgc tcggcgtgct gacagacgcc gaatattcgc gcctgcgagc cgaggcgctg
     6301 tcgcgcgtgc gcggtaccgt tcaggccgac atcctgaaag aggaccaggc gcagaacacc
     6361 tgcattttct ccacggagtt cgcgttgcgc atgatgggcg acgtgcagca gttcttcagc
     6421 gacaacgcgg tgaagaattt ctattcggtg tccatctcgg gttatcacat tgccgaggcc
     6481 ggcgcgaacc cgatcacgca gctggcgttc acgctcgcga atggtttcac catcgtcgag
     6541 tactaccttg cgcgtggcat gaagatcgac gatttcgcgc cgaacctgtc gttcttcttt
     6601 tcgaacggca tggacgcgga atacgcggtc atcggccgcg tggcgcggcg catctgggcg
     6661 cgcgcgatgc gcgatgtcta caaggcgggt ccgcgcagcc agatgctgaa gtaccacatc
     6721 cagacttcgg gccgcagcct gcatgcgcgg gaaatccagt tcaacgacat ccgcaccacg
     6781 ctgcaggcga tgtacgcgct gttcgacaac tgcaactcgc tgcacaccaa cgcctacgac
     6841 gaggcgctga cgactccgac cgaggagagc gtgcgccgcg cggtcgccat ccagctcatc
     6901 atcaatcgcg agttgggtct gaaccgcatc cagaatccct ggtcgggttc ctatgcgatc
     6961 acgtcgctga ctgacgtggt cgaagaggcc gtatacaaag agttcgaatc gctggccgaa
     7021 cgtggcggcg tgctcggcgc catggaaacc atgtaccagc gcggcaagat ccaggaagag
     7081 agcatgttgt acgagacgcg caagcacgat gggaccttgc ccatcgtggg cgtgaacaca
     7141 tttctcagcg agcatggtga aggcaagcag gacgtcgcgc cgctgtttcg tgccagtgac
     7201 gaggagcagc gcgcgcaagt cgacgcggtg cacgcgttcc atgagcgcca ctcccggtcc
     7261 gcgccggaag cgttgcggcg gctgcagtcg gtcgcggcga gcgggggcaa tgtattcgcc
     7321 gaactcctgg agaccgtcca ggtgtgctcg ctcggccaga tcacgcgcgc gctctacgaa
     7381 gtcggcggcc aataccgtcg gcacatgtaa cgcggttcag ctgcgcgcga attcgtcgat
     7441 ctcggcaacc gccgccttgg cgcgcgcgat gctgtgactg agcagaaagg cgtccatcca
     7501 cgccgcgagc ctgccgggat ggcgacgggc ccagtagtgc agaaggggtg gcgcaagtcc
     7561 gcccgccagg cacaccacga agttggccag gatgaaaccg gacgcgcgtt cccacagatc
     7621 gacgcccgcg aatcccatga cgatggcgac catcgccgcc ggcaccagca cccagcaggc
     7681 aaaccacaca gcgtgaaacg agcgggcttc gaattcctgc agcgtcgcca gcgagcgctg
     7741 gatcgtgagc accggctgcg tgtagttgat ccgcagtacg aggaccaggc gcaccacgcc
     7801 gatcacgatg agaccgacgc tcgccaggtg cagcgcaatg cccgcgatgg ccacggcggg
     7861 ggtagtcaga tgggcgctcc agaatttgcc ccagaccagc gcgaagaatg cgccgatgat
     7921 catgttcatc accgcgccgg ccaataccgg cgccagtcgc cagcgcgcct tgcgcgagat
     7981 cgaatcggtc aatagccggt gcaggatgcg ctcgtcgacg cgacggtcga gttgccagga
     8041 agatttcagt tcatcgagtt ccatggtgtt tctccgcgta ggtgcgcagc ctgtccttca
     8101 ggcggttgat cttggtagtt agattggtga cggtgatccc gagaacctcc gacatgccgg
     8161 agtagggggt ttcttccagg tagagcagca ggacggcgcg gtcgagcgct gtttgggagg
     8221 cgatgaagcc acgcaggatt cgctggcctt cgtcgttgtc ggccgcatcc tcgacggcgg
     8281 gttcgtgcat gtcggcgtcg taggccaccg accgccttcg acgcggcgcc tcgctgcgca
     8341 gccaggagat cgcaacgttg agtgcgatgc ggtacatcca ggtggagaag agccgggcgt
     8401 gatcgtaggt cgggaacgcg cgccagagtt gtgccgcgat ttcctgcgcc aggtcttcga
     8461 cgtccgcggc ctcgcgcgca aaggcgtggg cgactttgaa gatgagccgc cggtggccat
     8521 cgagcagcgc tgcgaactcg gctccggatt cggcgttcat gtaggtcgac tgtgcatggt
     8581 ttctgccgcc atgattcgcg gcaggaagcc ggatgtcaca gtcgggcttg ctaggcggcc
     8641 agatccggct cgccgagttc ccgcaggatc gcctggcggg actgatcgcg aagcttcaag
     8701 gcgggatcca cttccggtcc ttcgaccgtc ggcgcgatcg gcagcccgaa acgcacttcg
     8761 agcgggcccg gccttggcag cacgcgagtg gccggcatat tgcgccgcgt gccgaggatc
     8821 accgccggaa cgaccgggca ggccgcgcgt gccgcgatgg caaacgcgcc cgtgtggaat
     8881 ttcccgaggc ccacttcgct cgtgaaggtg ccttcgggaa aaaacaccag tgaatgcccg
     8941 ctggccgcgg tgcgcaaaac gcggcgagcg tccgtgccgc ccttatggcg attgaaacgg
     9001 tcgacgaact ccgagccaat gcgccgcagc agaagtcccc cgaccggaac gtcgttcatc
     9061 tcgcgcttga tcacgaaccc gaaacgcggt ggcagtgcgg ccgtcatgac tacgccatcg
     9121 agataactcg cgtgattggc gaccacgacg cactgaccgg cgggcagatt ctcggtgccg
     9181 cgcagacgga ggggcatgcc ggccaggagg aagaagcttc ttgctgcgat gcgggcggtg
     9241 ccgcgccgca actcgagcgt cggcatcagc aggacgccca gcagcgctgt aacgactaca
     9301 acgaggaaca gcagcgcggc gtagattcca tagatgaatc gcaggggaac ggagatcacc
     9361 gcgggccagg gcgagcgcgc ggggggcgaa gtcgatgtca tgcgcaggtg gtcgccaaga
     9421 agaatctcgg attcatgtga actggttctc tttatgttaa tgcttttgcc ggggtgtgaa
     9481 ggtcatcacg cctaagcgat acgccccgtc cccatactgc cgtgacgcgt taatccgcat
     9541 acgtattaag gcaatcacgt cttggtcaat caagctaaag cgcagaacga agcgcctgcc
     9601 gccgacaccg ccgacgccac cgtctcgaaa tttctcgacg cggtctggat ggaacgcgga
     9661 ctgtctgcga acacgctcgc cgcctatcgt gccgacctca ccgcgctcgc gcgctggctg
     9721 gcggaacggg gcacgcccat catcaagacg acccgcgcgg atctgctcgg gttcatcgcg
     9781 taccgcgtcg aagccggtgc gcgaccacgc tccaccgcgc gacagctttc gtcgttccgt
     9841 cgtttcttcc gctacttcgt tcgcgaagga gtgatccgcg atgatccgac cgctcagatt
     9901 gcgatgccga agatcggccg ctccctgccg aagtcgctca ccgaagaaga ggtcgagtca
     9961 ctgctgaacg cgccggccat cgccgacccg ctcggacatc gcgaccgcac catgctcgaa
    10021 gtgctctacg ccacgggcct gcgcgtttcg gaactcgtga acctcaagta caaccagatc
    10081 aacaccaacc agggtgtcat ccggatcatg ggcaagggca atcgcgagcg tttgattccg
    10141 ctcggcgagg aagccgtgcg ctggttgacg gacttcgtgc gcgctccgcg cagcgagatc
    10201 ctgctcgacc gcaccaccga ctatctgttt ccgacgcgcc gcggcgatcg catgacgcgc
    10261 caggccttct ggcacatcat caagcgatac gcgcgcaagg ccgtcatcga caaggaactg
    10321 tcgccgcaca cgctgcgtca cgcctttgcc acacacctgc tcaatcatgg cgccgacctg
    10381 cgcgtcgtgc agatgctgct cggtcactcg gacctctcga ccacgcagat ctacacgcac
    10441 gtggcccgcg agcgcatgaa agagatgcac gggacgcatc acccgcgcgg gtgatgccgg
    10501 tcggcgccgc gttcagcggc gccttcgacg cagcgggcgc cgtatttcct ggg
//
LOCUS       DATDEU010000368         6294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1381130, whole genome shotgun sequence.
ACCESSION   DATDEU010000368 DATDEU010000000
VERSION     DATDEU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381130"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2332
                     /locus_tag="VIV63_08725"
     CDS             <1..2332
                     /locus_tag="VIV63_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY6124721.1"
                     /translation="MGFALVVVFAALIAPVAAHAQSKIPRPEGIQSDVNFWIRVYTEV
                     NTNEGFLHDERNLAVVYDTLKFGAGGSSRERQRIVDERRDRHVAALRRIIAALPTEAG
                     REALSAEDKRLLALFGPNPSAIILKEATTRIRFQLGQADRFKEGLIRSSSWETHIAET
                     FANQGLPPELAVLPHVESSFNAAAYSKVGAAGLWQFMRSTGRRYMRVDDAVDERLDPY
                     RSTEAAAQLLAYNYRVLGSWPLALTAYNHGAAGMRRAKESVGTDDFVKINRTYTSRTF
                     GFASRNFFPSFRAALTIDENPEKYFGALERRPEQKFHEITMPAYVRLSTLERTLDVDR
                     EQLRVLNPGWRPTIFNGTRLVPRGYRLRLPAETKGQWTAELLSARLPANELYAGQITP
                     RTHRVRKGESMATIAQRYGMSVSRLAEMNGMKSGAPLRAGRHLQLPEQLPRTLSGTAP
                     AASQATATVAAAEAAAPSPANATASSAPVGEFYVVRRGDSLQAIAARVSVPEAQLLKM
                     NKLRNPDFLYEGQRLRIAGEAVAVTQAEAEVKVAAIDAARGEAQREGAAVEVVREETT
                     RPIGTGEPTRGRSRSAAAVAMAAATTPEVASSVVQAAESAREPVSAAQAEALSPALGP
                     VSVSQGLADSIDYQVRDDGSIRVEATETLGQYADWLQIPTQKLRNVNKLKARQPVLLG
                     QKLNLDYSRVSREAFEQVRRDYHAKLQGEFFLQHRIAGTEVYIVRRGDSLWTMTQKFS
                     NLPIWLLRQYNPDTDLSDLRAGTQVVMPRVEVVASS"
     gene            complement(2329..2910)
                     /locus_tag="VIV63_08730"
     CDS             complement(2329..2910)
                     /locus_tag="VIV63_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124722.1"
                     /translation="MTSRRDTTTTMRLVRFIQVGLATALLGACAALPDDAPIVEKLDT
                     ETGTTVTRLGRPVEIYRETFLQETAGRFAFIGPFETNLMGRRELYVWLAVPIEPMADA
                     VPVVEVNGAVLPLGTPGRSADYANVHESPYKIPTPWSAMYYFKVDETVIARLGEATDV
                     NVRVVEAGKNGPIKALFAVKIAEDPRLRDFATR"
     gene            3031..3582
                     /locus_tag="VIV63_08735"
     CDS             3031..3582
                     /locus_tag="VIV63_08735"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY6124723.1"
                     /translation="MSLATRILALAAPILACAFVGTASGDALPYHARAMASMGVELPV
                     ADRVLVRKSERRLYLMRGDDVLRAYRVALGLNPGGAKERSGDFRTPEGSYRLTRRNTR
                     SDFFLSIQVSYPNDQDAQRARRNGWQPGGSIMIHGLPNDPRHSPDYYASQDWTDGCIA
                     VTNADMVEIWMMTSENVRIDIQP"
     gene            3665..5050
                     /gene="ppnN"
                     /locus_tag="VIV63_08740"
     CDS             3665..5050
                     /gene="ppnN"
                     /locus_tag="VIV63_08740"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF038390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HEY6124724.1"
                     /translation="METSIVIPDFVDARIGPRGSLENLSQAEIEKLLDSRAGGLYPLF
                     RKCALAVLNSGSETDNAKEIFDLYKDFEVELVRQAWGVKLEIKNAPGQAFVDGEMIRG
                     MKEHVFAVLRDVIFISNEIMESGRFDLEKPAAITNAVFHILRNARILDYKAKPNLVVC
                     WGGHSIGTEEYQYTKKVGYELGLRGLDVCTGCGPGAMKGPMKGATIGHAKQRIVGGRY
                     IGLTEPGIIAAEPPNPIVNQLVIMPDIEKRLEAFVRLAHGVVVFPGGVGTAEEILYLL
                     GILLEPSNSGQPLKVVLTGPKNSADYFVQIKNFIEATLGKKAADLLHVIVDDPAEVAR
                     YMVSTMEQARDFRRTNSDSYNYNWLLKIPPAFQHPFEVTHESMRALRLYRDLPAHELA
                     ADLRRAFSGIVTGNVKENGVRAIEKLGPYELTGDASVMKLLDELLTAFVAQKRMKLPG
                     SAYRPCYRLVA"
     gene            5297..5464
                     /locus_tag="VIV63_08745"
     CDS             5297..5464
                     /locus_tag="VIV63_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124725.1"
                     /translation="MGTDAYAAWLERMQRTRGRHAAITKNLYTWSSYKHWADKVKGSW
                     EDEPVPAPSKK"
     gene            complement(5599..>6294)
                     /locus_tag="VIV63_08750"
     CDS             complement(5599..>6294)
                     /locus_tag="VIV63_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124726.1"
                     /translation="GDATLLIDRDPQGSATRWLRKRKPPQSVITGIATFEHDARTTRS
                     WQMRVPEGTDKIVVDSPAAVEARLMPELTRDANKIIVPVLPSDIDIHAASRCIADLLL
                     VAKIKRVENRIGVIANRVRKNTLMFQSLMRFLEKLEIPIVATMRDSQNYVRAAEQGVG
                     IHEMKPYLVKEDMGQWETLLDWLEPERVLAEQPAPLQEQPAPEVVAVSTEAAIEDTDG
                     TAAHDAVFITQSW"
BASE COUNT         1169 a         2033 c         2000 g         1092 t
ORIGIN      
        1 gatgggattc gccctcgtgg tcgtcttcgc cgcgctgatc gcgcccgtcg cggctcatgc
       61 gcagtcgaag atcccgcgtc cggaaggcat tcaatccgac gtgaacttct ggatccgcgt
      121 gtacaccgag gtcaatacca acgagggttt cctgcacgac gagcgcaatc tcgcggtggt
      181 gtacgacacg ttgaagttcg gtgccggcgg ttcatcgcgc gagcgccagc ggatcgtgga
      241 tgagcggcgc gaccgtcacg tcgccgcctt gcgccgcatc atcgcggcgc tgcccaccga
      301 ggcggggcgt gaggcgctct ccgccgaaga caaacgtctg ctcgcgttat ttggtccgaa
      361 tcccagcgcc atcatcctca aggaggcgac cacgcggatc cgcttccagc tgggccaggc
      421 cgatcgcttc aaggaaggtc tgatccgttc ctcgagctgg gaaacccaca tcgccgagac
      481 gttcgcgaac cagggcctgc cgccggagct cgcggtgctg ccgcacgtgg agtcgtcgtt
      541 caacgccgcg gcctactcga aggtgggcgc cgcgggactg tggcagttca tgcgatccac
      601 cggccggcgt tacatgcgcg tcgacgatgc agtcgacgag cgcctggacc cgtaccgttc
      661 gaccgaagcc gcggcccaac tactggcgta caactaccgc gtgctcggaa gctggccgct
      721 cgcgctcacc gcgtacaacc acggcgccgc cggcatgcgc cgcgccaagg aatccgtggg
      781 cacggacgac ttcgtgaaga tcaatcgcac gtacaccagc cgcacattcg gtttcgcgtc
      841 gcgcaatttc tttccctcgt tccgggccgc gctgaccatc gacgagaatc ccgagaaata
      901 tttcggcgcg ctcgaacgcc gccccgaaca gaaattccac gagatcacca tgccggccta
      961 cgtgcggctg tcgacgctcg aacgcacgct cgatgtcgac cgcgagcagc tgcgcgtgct
     1021 gaatcctggc tggcgtccca cgatcttcaa cggcacgcgg ctcgtgccgc ggggttatcg
     1081 cctgcgtctg cccgccgaga cgaagggtca gtggactgcc gaattgctga gcgcgcgcct
     1141 gcctgccaac gagctgtatg cggggcagat cacgccgcgc acgcatcgtg tgcgcaaggg
     1201 cgagagcatg gcgaccatcg cgcagcgtta cggcatgtcg gtgtcgcggc tcgcggagat
     1261 gaatggcatg aagtctggcg ccccgcttcg cgccgggcgc cacctgcaat tgccggaaca
     1321 attgccgcgc acgttgtcgg gaacggctcc ggcggcgagc caggcaacgg ccaccgtcgc
     1381 cgcggctgaa gcggccgcgc cgtcgccggc caacgcgacc gcgtcgagtg cgcccgtcgg
     1441 cgagttctac gtggtgcgtc ggggcgattc actgcaggcg atcgcggcgc gcgtgagcgt
     1501 gcccgaagcg cagctgctga agatgaacaa gctgcgcaat ccggatttcc tctatgaagg
     1561 ccagcgactg cgcatcgcgg gcgaggccgt ggcagtcacg caggcggaag ccgaagtgaa
     1621 ggtggcggcc atcgacgcgg ctcgcggcga agcgcagcgc gaaggcgcgg ccgtcgaagt
     1681 cgtgcgcgaa gaaaccacgc gaccgatcgg caccggtgag ccgacgcgtg gcaggtcacg
     1741 ctcggcggct gccgtggcga tggctgcggc caccacgccg gaagtggcgt cgtcggtcgt
     1801 gcaggcggcg gaaagcgcgc gcgagccggt gtcggccgcg caggccgaag ccctgagccc
     1861 ggcgctcgga ccggtttccg tatcgcaggg cttggccgat tccatcgact accaggtgcg
     1921 cgacgacggc tcgatccgtg tcgaagccac ggaaacgctg gggcaatacg ccgactggct
     1981 gcagatcccc acgcagaagc tgcgcaacgt gaacaagctc aaggcgcgtc agcccgtgtt
     2041 gctgggccag aagctgaacc tcgactattc gcgcgtgtcg cgcgaagcgt tcgagcaggt
     2101 gcgccgcgac tatcacgcca agctgcaggg tgagttcttc ctgcagcacc gcatcgccgg
     2161 caccgaggtc tacatcgtga gacgcggcga ttcgctgtgg accatgacgc agaagttcag
     2221 caacttgccg atctggctgc tgcgccagta caaccccgac acggatctct ccgacctgcg
     2281 tgccggcacg caggtggtga tgccgcgcgt ggaagtggtc gcgtctagct agcgggtggc
     2341 gaaatcgcgg aggcgcgggt cctcggcgat cttcacggcg aacaacgcct tgatcgggcc
     2401 gttcttgccc gcctcgacca cgcgcacgtt cacatcggtg gcttcgccta gccgcgcgat
     2461 gacggtctcg tccaccttga agtagtacat ggcgctccag ggcgtcggaa tcttgtacgg
     2521 cgattcgtgg acgttcgcgt aatccgcgct cctgcccggc gtgccgagcg gcagcaccgc
     2581 gccattcact tcgacgacgg gaaccgcatc cgccatcggc tcgatgggca ccgccagcca
     2641 gacgtaaagc tcgcgccgtc ccatgagatt ggtctcgaac gggccgatga acgcgaaccg
     2701 gccggccgtc tcctgcagaa aagtttcgcg atagatctcc acggggcggc cgagccgtgt
     2761 gaccgtggtg cctgtttcgg tgtcgagctt ttcgacgatg ggggcgtcat cgggcaatgc
     2821 cgcgcaagca ccgagcaggg cggtcgccag cccaacctgg atgaaacgca ccagccgcat
     2881 cgtggtcgta gtgtcgcgac ggcttgtcat aatcgaattg gatagacatt cttgctgcat
     2941 tgaacacccc aagctgtgac gcgctccgtt attggcggcg gctccagcct ctataattta
     3001 ttcaactaca agtgcttgtt ttgtaatcaa atgtcgcttg ccacccgaat actcgcactc
     3061 gccgcaccga ttctcgcctg cgcgttcgtt ggaacggcct cgggcgatgc gcttccttat
     3121 catgcccgcg ccatggcctc gatgggcgtc gaattgcccg tcgccgatcg cgtgctggtc
     3181 cgcaaatccg agcgccgtct ctatctcatg cgcggcgacg acgtgttgcg cgcctaccgc
     3241 gtcgcgctcg gcttgaatcc gggtggagcg aaagagcgct ccggcgattt ccgcacgccc
     3301 gaaggcagtt accgcctcac gcgtcgcaac acgcgcagcg actttttcct ctcgatccag
     3361 gtctcctatc ccaacgacca ggacgcgcag cgcgcgcgcc gtaacggctg gcagccgggc
     3421 ggttcgatca tgatccacgg gctgccgaat gatccgcgcc actcacccga ttactacgcc
     3481 tcgcaggatt ggaccgatgg ttgcatcgcc gtcaccaatg ccgacatggt cgaaatctgg
     3541 atgatgacgt cggaaaacgt gcggatcgac atccagccgt aagctctccg ggccgcgaga
     3601 cgcggctccc ctgtcgagta cactgctcac cacaaacaaa ggtgaacaaa ctctagggga
     3661 tatcttggaa accagcatcg tcattcctga cttcgtggat gcgcgcatcg gaccgcgcgg
     3721 cagcctggaa aatctctccc aggccgagat cgaaaaactc ctggattcgc gtgccggcgg
     3781 cctctatccg ctgtttcgca agtgcgcgct ggccgtgctc aattccggca gcgaaaccga
     3841 caacgccaag gaaatcttcg acctctacaa ggacttcgaa gtggagctgg tgcgccaggc
     3901 ctggggcgtc aagctcgaga tcaagaacgc gccgggacag gcgttcgtcg acggcgagat
     3961 gatccgcggc atgaaggaac acgtcttcgc ggtcctgcgc gacgtcatct tcatcagcaa
     4021 cgagatcatg gaaagcggcc gcttcgacct cgagaagccg gcggccatca ccaatgcggt
     4081 gttccacatc ctgcgcaacg cgcggatcct cgactacaag gcgaaaccca atctggtggt
     4141 ctgctggggc ggccactcga tcggaaccga ggagtaccag tacaccaaga aggtgggcta
     4201 cgaactcggg ctgcgcgggc tcgatgtctg cacgggctgc gggccgggcg ccatgaaggg
     4261 cccgatgaag ggcgcgacga tcggccacgc caagcagcgc atcgtgggcg ggcgttacat
     4321 cggtctcacc gagcccggga tcatcgccgc cgagccgccg aaccccatcg tcaaccagct
     4381 cgtgatcatg ccggacatcg aaaaacgcct cgaggcgttc gtgcggctcg cgcatggcgt
     4441 cgtggtgttc cccggaggcg tcggcacggc cgaggaaatc ctctacctgc tcggcatcct
     4501 gctcgaaccc tcgaacagcg ggcagccgct gaaagtggtg ctcacggggc cgaagaactc
     4561 ggccgactac ttcgtccaga tcaagaattt catcgaggcg accctcggaa agaaggccgc
     4621 cgacctgctg cacgtgatcg tcgacgaccc ggccgaagtc gcccgctaca tggtctcgac
     4681 catggagcag gcgcgcgatt tccgccgcac caacagcgac tcgtacaact acaactggct
     4741 gctgaagatc ccgccggcgt tccagcaccc cttcgaagtg acgcacgaat ccatgcgtgc
     4801 gctgcgcctg taccgcgacc tgccggcgca cgagctggcc gccgacctgc gccgcgcgtt
     4861 ctccggcata gtcaccggca acgtgaagga aaacggtgta cgcgccatcg aaaagttagg
     4921 cccctacgag ctcaccggcg atgcgtcggt gatgaaactg ctcgacgaac tactgaccgc
     4981 gttcgttgcg caaaagcgaa tgaagcttcc tggaagcgct taccggcctt gctacaggct
     5041 ggtggcctga cggcgaatta cacaataacg cgctgcatcg ttaaatcgcg cggctctttt
     5101 cgagtcgcgg cagaacataa cgaaaaaatc tgtgttaacc agcgcgcatc ggccgcaagc
     5161 cgtcgcgggc gcgtgaatca gttcctgtcg tttcggtgcg acgtaatcgt aggcttacgt
     5221 caaccgaaag ggagtaacgc acatgagtgc ctcagacaga aaccggggaa atctagcgaa
     5281 cggcacaccg cctccggtgg gcaccgacgc ttacgcagcc tggctcgagc gcatgcagcg
     5341 cacgcgcgga cggcacgcgg ccatcaccaa gaacctctac acgtggtcta gctacaagca
     5401 ttgggctgac aaggtgaagg gttcgtggga agacgagccg gttccggccc cgagcaagaa
     5461 gtaagggtca gacgacgagt tcgataacac cgcgtatcaa acgcgggtgc gtaaaagggg
     5521 acagatctat ttattgaatc tgcctcggtg ttacgcaagg ggactctcca ataaatagat
     5581 ctgtcccctt ttaggggatt accagctttg ggtgatgaac accgcgtcgt gcgcggccgt
     5641 gccatcggtg tcttcgatgg ctgcctcggt ggaaaccgcc acgacttcgg gcgcgggttg
     5701 ttcctgcagg ggagcgggct gctccgccag cacccgttcc ggctcgagcc agtccagcag
     5761 cgtctcccac tggcccatgt cttccttgac caggtacggc ttcatctcgt ggatgccgac
     5821 gccctgttcg gccgcacgca cgtagttctg cgagtcgcgc atggtcgcga cgatggggat
     5881 ctcgagcttt tcgaggaacc gcatcagcga ctggaacatc agcgtgttct tgcgcacgcg
     5941 gttggcgatg accccgatgc ggttctccac gcgcttgatc ttggcgacca gcagcaggtc
     6001 ggcgatgcag cgcgacgcgg cgtggatgtc gatgtccgag ggcagcaccg ggacgatgat
     6061 cttgttcgcg tcgcgcgtga gctccggcat gagccgcgct tcgacggcgg cggggctgtc
     6121 caccacgatc ttgtccgtgc cttcgggcac gcgcatctgc caggaccgcg tggtgcgcgc
     6181 gtcgtgctcg aaggtggcga tccccgtgat gaccgactgc gggggtttgc gcttgcgcag
     6241 ccatcgcgta gccgaccctt gaggatcgcg atcgatcagc agggtggcat cgcc
//
LOCUS       DATDEU010000369         3456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1964116, whole genome shotgun sequence.
ACCESSION   DATDEU010000369 DATDEU010000000
VERSION     DATDEU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964116"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VIV63_08755"
     CDS             <1..911
                     /locus_tag="VIV63_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY6124727.1"
                     /translation="IAINPTWNIVAGQLSLTMDYKVYDAAATKVLEGTKVQSAALNAP
                     GPLGGFPNAALRTTQLVLVDVLAKLSPSAAKFPATAQMTAINRELLIDRKKAASTGTA
                     FYINKSGQMMTAAHVLRDCVVLEAQKDGVTFPLKLSASSNLLDLAVVESSKPTDRALP
                     LRIGQTITLGEGVTNVGYPLQGLLADSPNLTRGNVSARAGMKGSVGLFQFSAPIQPGS
                     SGGPVVSDGGELLGVTVSSLNAAALIKDGLLPQNVNFALEAKYAAMFLRDSHVDFNEV
                     KPRADGNMSVANEAALGAVLQLSCYQ"
     gene            908..1450
                     /locus_tag="VIV63_08760"
     CDS             908..1450
                     /locus_tag="VIV63_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124728.1"
                     /translation="MNLKISVGLVGLLLSFPAMADDPALPAMSFYGSIASDQLFDLVK
                     KSDQFAKLDKELYGSPLTLRVMHSLQPTSGGKATGLLSAIFSGGTLGLLPVVTNNSLV
                     VTYEVRVHGRELVSYSYQRSFTRAVNIWAKDETYGLGKDGLEWLKSTAGEFTAAAATD
                     AKLIALKKEYERYFAAPQPP"
     gene            complement(1465..2934)
                     /locus_tag="VIV63_08765"
     CDS             complement(1465..2934)
                     /locus_tag="VIV63_08765"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY6124729.1"
                     /translation="MPVLDVAAYESTLSDPIAHTDTWVLPNGILTQPAPAAVESTMEM
                     PALSPAQKPAYDLDHSGTHEMPPMLIQTPKASRKRAKSAPPVTTSPSSEYDTAAAAVA
                     APVAAPSIVLPPSPPLIEELRNALAAAERRVEELNERARIADAERTVAVARANAESAQ
                     LREQLAAHLESLHTAKESLSVQRADLPDLEDQLFARADRIAALEKIQATHAAELAGKA
                     ALLAKSEQRCEALDCDAMNLRAAVARRDAQIATLEADLAARQQRESKLSARLADALAS
                     TDPHVPQLRSEINVRDEQIRLLKLDAELARTQTAEKDHDLQVAEESIRNLEVEVRDKN
                     AKLEEATVTVEEWRAVIAESQRSILQRDGRIQQLETDLEKRVAVATDSAAQTGEEIAL
                     EGPARVLIRTNGNTDYVHVLGRRTRIGRGSDNELVLDTKHISRYHAVLLAGPVHTSIE
                     DLNSTNGVFVNGKRVSRQVLKDGDRVTVGRTQFRYSVRD"
     gene            3173..>3456
                     /locus_tag="VIV63_08770"
     CDS             3173..>3456
                     /locus_tag="VIV63_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6124730.1"
                     /translation="MDITPPRLRRAYFECRHGQLHVHNAIPVGGGFDELTTLICLHGA
                     TGTGRMFLEVSRQLGSSRSIYSPDLPGCGESDASPTALSVAAYACAIADFL"
BASE COUNT          564 a         1100 c         1141 g          651 t
ORIGIN      
        1 tgatcgcgat caatcccacc tggaatatcg tggccggcca gctgtcgctg accatggatt
       61 acaaggtgta cgacgccgct gccaccaagg tgctcgaggg cacgaaggtg cagagcgcgg
      121 cgctgaatgc gccgggaccg ctgggcggtt tcccgaatgc cgcgctgcgc accacccagc
      181 tggtgctcgt cgatgtgctg gcaaagctct cgcccagtgc cgcgaaattt ccggccactg
      241 cgcagatgac cgccatcaat cgggaactgc tgatcgaccg caagaaggcg gcgagcaccg
      301 gcacggcctt ctatatcaac aagtctggcc agatgatgac cgccgcgcac gtgctgcgcg
      361 attgcgtggt cctcgaggcg cagaaggacg gagtcacctt cccgctcaag ttgtccgcga
      421 gcagcaacct gctcgacctc gcggtggtgg aaagcagcaa gcccaccgat cgtgcactgc
      481 cgttgcggat cgggcagacg atcacgctcg gcgaaggcgt caccaacgtg ggctatccgc
      541 tgcaggggct gctggccgat tcgcccaatc tcacgcgcgg caatgtcagc gcgcgagcgg
      601 gcatgaaggg ctccgtgggc ctgtttcaat tctccgcgcc catccagccg ggatcgagcg
      661 gcggacccgt ggtctccgat ggcggcgaac tgctgggcgt gaccgtgagt tcactgaacg
      721 ccgcagccct catcaaggac gggctgttgc cgcagaacgt gaatttcgcg ctcgaagcga
      781 agtacgccgc gatgttcctg cgcgattcgc acgtcgactt caacgaagtg aaaccgcggg
      841 cggatggcaa catgagcgtc gcgaatgaag cggcgctggg cgcggtactc caactgagct
      901 gttaccaatg aacctgaaaa tcagcgtggg cctcgtgggc ctcctgcttt cgtttcccgc
      961 gatggccgac gatcccgcgt tgcccgccat gagtttttac gggtccatcg ccagcgacca
     1021 gctcttcgac ctggtgaaga agagcgatca gttcgcgaag ctggacaaag agttgtatgg
     1081 cagtccgctc acactgcgcg tgatgcattc actgcagccc acctcgggcg gcaaggccac
     1141 cggcctgctc tccgcgatct tcagtggcgg cacgctcggg ctgttgcccg tggtcaccaa
     1201 caacagcctg gtggtcacct acgaagtgcg cgtgcacggc cgtgagctgg tgtcgtactc
     1261 ctatcagcgc tctttcacgc gcgcggtgaa catctgggcc aaggacgaga cctatggtct
     1321 cggaaaagac gggctggagt ggctcaaatc gaccgcgggc gaattcaccg cggcggcggc
     1381 cacggacgcc aagctgatcg cgctcaagaa ggaatacgag cgctacttcg ccgcgccgca
     1441 gcccccatag gccggggtga gtagctagtc gcggacgctg tagcggaact gcgtgcggcc
     1501 gacggtgacg cggtcgccgt ctttcagcac ctggcgcgag acgcgcttgc cgttcacgaa
     1561 cacgccgttg gtgctgttca gatcctcgat cgaggtgtgc accgggcccg ccagcagaac
     1621 cgcgtgataa cggctgatgt gcttggtgtc gagcacgagt tcgttgtcgg agccgcggcc
     1681 gatgcgcgtg cggcggccga gcacgtgtac gtaatcggta tttccattgg tgcggatcag
     1741 cacgcgcgcc ggaccttcca gcgcaatctc ttcaccggtc tgcgccgcgg agtcggtggc
     1801 cacggcaacg cgcttctcga ggtccgtttc gagctgctgg atgcggccgt cgcgctggag
     1861 gatggaccgc tggctctcgg caatgaccgc tcgccattcc tcgacggtga cggtggcttc
     1921 ttcgagcttg gcgttcttgt cgcgtacttc gacttccagg ttgcggatgc tctcttccgc
     1981 gacctgcagg tcgtgatctt tttcggccgt ctgcgtgcgg gcgagctccg cgtccagctt
     2041 gagcaggcga atctgctcgt cgcgcacatt gatctcgctg cgcagctgcg gcacgtgagg
     2101 gtcggtggag gccagcgcgt cggcgaggcg cgcgctcaac ttcgattcgc gctgctgccg
     2161 cgccgccagg tcggcttcga gcgtggcgat ctgggcatcg cgccgcgcca cggcggcgcg
     2221 caggttcatg gcgtcgcaat cgagcgcttc gcagcgctgc tcgctcttcg ccagcaacgc
     2281 cgctttcccg gccagctccg cggcgtgcgt cgcctggatc ttttcgagcg cggcgatgcg
     2341 atccgcgcgc gcgaacaact ggtcttcgag gtcgggcagg tccgcgcgct gaacgctcag
     2401 gctttccttc gcggtgtgca gtgattcgag atgtgcggcc aactgttcgc gcaactgggc
     2461 gctctcggcg ttggcgcgcg ccacggcgac ggtgcgctcc gcatcggcga tgcgcgcgcg
     2521 ttcgttgagt tcttcgacgc ggcgctcggc ggcagccagc gcattgcgca gctcttcgat
     2581 gagcggcggc gaaggaggaa ggacgatcga tggcgcggca acgggcgcgg ccactgccgc
     2641 cgcggcggta tcgtattcgg atgacggcga agtcgtgacc ggcggcgcgg acttggcgcg
     2701 ctttcgcgaa gctttcggcg tctggatcaa catcggcggc atttcgtgcg tgccgctgtg
     2761 gtcgaggtcg taggccggct tctgcgccgg cgacagcgcc ggcatttcca tcgtggactc
     2821 gacagcagcc ggtgcgggct gagtcaggat tccattcggg agaacccacg tgtcggtgtg
     2881 cgcaatggga tcgctcagcg tcgactcgta ggcagcgacg tccagaacag gcaattcagc
     2941 cgtagcttca ttgtcgacgt ccgacgggga cgcgctcgta ttcaccggag gcatcgctgg
     3001 gaatccccaa ttattcgatt tctgcgagga ttctagcccc cgtgccgaag aggatgtttg
     3061 aagagttttc gtgcccatgc ggaaatgcga aggcgcttct cagttctgtc aaatccccct
     3121 aagcacggct ccgaaagttc gccaggccgg cgttgggtta aggttgcgtc ccatggacat
     3181 caccccaccg cgcctgcggc gcgcgtattt cgagtgccgc cacggccagc tccacgtcca
     3241 caacgccatc cccgtgggtg gcgggttcga cgagctgacc actctcattt gcctgcacgg
     3301 cgcgaccgga accgggcgca tgttcctcga ggtatcgagg cagctcggca gcagccggtc
     3361 gatttacagc ccggatctgc ccggctgcgg cgaatctgac gcctcgccaa cggcgctgtc
     3421 agtagctgcc tacgcctgcg ccatcgccga tttcct
//
LOCUS       DATDEU010000370         3744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_943629, whole genome shotgun sequence.
ACCESSION   DATDEU010000370 DATDEU010000000
VERSION     DATDEU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943629"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..2083)
                     /locus_tag="VIV63_08775"
     CDS             complement(11..2083)
                     /locus_tag="VIV63_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124731.1"
                     /translation="MKRNSLGWWLAASNVAIVLLVAAGISYFAIDMLRGLADSQQKIH
                     VQLAGANAREEITRMAEDTLTYARVLADRPPLGRLLGEPGREGLSPFLRRFCETSELD
                     ACAIFDGAQVVASSGEALPWPQIFTVAQEQGERFMAVPPGTQYSVVGAAAKLPGQYSS
                     FSVMAVHLLDESLAAVLAEHSGLPVRLINYRAFNATPDNEFTSLHSQALTDGRFAVQR
                     IDSLGLYASSFPVFSPSGEGIALIETRAPATETDRAVSALVWRLILTAFVFAVLALIA
                     GVLLGWRVTRPAEALTDAAVRLGQGDFATSIPTAGPAEIGQLARTMDDMRRNLVELNS
                     ELRHRDAEAQVVLEAVVEGVYAVDQERNIRYLNPQAARLLGIDAADAIGKFCGDVLKP
                     CGPDGSSRGTRPCATTACPILRARQAGSAQAVEQLQTNLGLRQAVITSSRVVNGIQVQ
                     VLRDETELEGVRRARDTVLANVSHEFRTPLAAQLASIELLREGLKTSPPEKLEELVLS
                     LERGTLRLTRLIDNLLESVRIESGQLDVRRQSIELADVVDDARLLVEALLTQRRQPLE
                     VDVPEGLELQADATRLTQVFVNLIANASKFAPEGSAVRVGARREGRQISAWVEDAGPG
                     LPEGDALSIFERFKRGGSLEPEPGGLGLGLWISRSIVERHGGTISAARTAEGFTRFTF
                     TLPTEVTA"
     gene            complement(2080..2955)
                     /gene="cysW"
                     /locus_tag="VIV63_08780"
     CDS             complement(2080..2955)
                     /gene="cysW"
                     /locus_tag="VIV63_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEY6124732.1"
                     /translation="MAGPSSHADVAAELATRPGHTNLRSALVRWLLITISLTFLGALL
                     AAPLAAVFAMALEKGWSAYFSSFADEDTRASIRLTLTVALIVVPLNTVFGITAAWCIA
                     KFEFRGKSFLITLIDLPLAVSPVVSGLIYVLLFGLNGWFGAWLQDHDISIVYALPGIV
                     LATMFVTFPYVARELIPLMQQLGNDEEEAAITLGAGGWFTFFRVTLPRIRWGLIYGVI
                     LCNARAMGEFGAVSVVSGNVRGLTNTMPLQIESLYNEYNMVGAFAVASVLSLLAVATL
                     IIKTLVEGRAGYRRR"
     gene            complement(2955..3743)
                     /gene="cysT"
                     /locus_tag="VIV63_08785"
     CDS             complement(2955..3743)
                     /gene="cysT"
                     /locus_tag="VIV63_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY6124733.1"
                     /translation="MGFTTFFLSAIVLLPLSALVLKTAGMDWSRFVEVIASPRALASY
                     KLSFGASLVAACVNMVFGFALAWTLVRYEFPGRKLVDAIIDMPFALPTAVSGIALTTI
                     FAANGWIGQYLEPRGIFVAYTPLGITVALTLIGMPFVVRTVQPALQEVQRDLEDAAET
                     LGAGRFYVFRRIIVPTVLPALLTGFTLAFARAVGEYGSVIFIAGNKFMKTEITPLLIV
                     FRLEEYDYAGAAALGFVMLAISFVMLLAINLIQVWGRKRLMVSR"
BASE COUNT          698 a         1230 c         1243 g          573 t
ORIGIN      
        1 accagaatct tcatgcggtc acctcggtgg gcagtgtgaa cgtgaaccgc gtgaagcctt
       61 cggcggtacg cgcagccgag atggtgcctc cgtggcgttc gacaatggac cgcgagatcc
      121 acagccccaa cccgaggcca cccggctcgg gctccagcga accgccgcgt ttgaatcgtt
      181 cgaagatgct cagcgcgtca ccctcgggca gacccggacc cgcatcctcg acccaggcgc
      241 tgatctgcct gccttcgcgg cgcgcgccca cgcgcacggc gctgccttcg ggcgcgaact
      301 tgctggcgtt ggcgatgaga ttgacgaaca cctgcgtgag acgcgtggcg tcggcctgca
      361 attcgagccc ctcgggcaca tccacctcga gcggctggcg ccgctgcgtc agcaacgctt
      421 ccacgagcag ccgggcgtcg tcgacgacgt cggcaagctc gatgctctgc cggcgcacgt
      481 cgagctgacc cgattcgatg cgcacgcttt cgagcaggtt gtcgatgagc cgcgtgaggc
      541 gcagcgtgcc gcgctccagc gacagcacca gctcctcgag cttctccggc gggctggttt
      601 tcaatccttc gcgcaacagt tcgatggacg cgagctgcgc cgcgagcggc gtgcggaact
      661 cgtgcgagac gttggccagc acggtatcgc gcgcgcgccg tacgccttcc agttcggtct
      721 cgtcgcgcag gacctgcacc tggataccgt tcaccacgcg cgaactggtg atgacggcct
      781 ggcgcaatcc caggttggtc tgaagttgct ccacggcctg cgcactgcct gcctgacgcg
      841 ctcgcaggat cgggcacgcc gtggtcgcgc agggtcgcgt ccctctggac gaaccatcgg
      901 gcccacaggg cttcagcacg tcaccgcaga acttgccgat ggcatccgcg gcgtcgatgc
      961 ctaacaaccg cgcggcctgg gggttgagat agcggatgtt tctttcctgg tcgacggcgt
     1021 atacaccttc gaccacggcc tcgagcacca cctgcgcctc ggcgtcgcga tgccgcagct
     1081 cggaattgag ttccacgaga ttgcgccgca tgtcgtccat ggtgcgcgcg agctggccga
     1141 tttccgcggg accggccgtc ggtatggacg tggcgaaatc gccctgcccc aggcgcaccg
     1201 ccgcgtcggt caatgcttcg gccggccgcg tgacgcgcca gcccagcagc acaccggcaa
     1261 tcaatgcgag cactgcgaat acgaacgcgg tcaggatgag gcgccacacg agcgcgctca
     1321 cggcgcgatc ggtctcggta gccggcgcgc gggtctcgat cagcgcgatg ccttcgccgc
     1381 tcggcgagaa caccgggaag ctggacgcat aaaggccgag gctgtcgatg cgctggaccg
     1441 cgaaacgtcc gtcggtcagc gcctgcgaat gcaacgaggt gaattcattg tcaggcgtgg
     1501 cattgaacgc gcggtagttg atgagccgta ccggcaggcc gctgtgctcc gccagcaccg
     1561 cggccagcga ttcgtcgagc aggtgcacgg ccatgacgct gaacgaactg tactgacccg
     1621 gtagtttggc ggcggcgccg accacggaat actgtgtgcc gggcggcacc gccatgaatc
     1681 gctcaccttg ttcctgggcc acggtgaata tctgcggcca cggcaacgcc tcgccgctcg
     1741 acgccaccac ctgcgcgccg tcgaatatgg cgcacgcatc cagttcggag gtctcgcaga
     1801 accggcgcag gaacggcgac aggccttcgc gcccgggttc gcccagcaac cggccgagcg
     1861 gcgggcggtc ggcgagcacg cgcgcgtagg tcaatgtgtc ttcggccatg cgcgtgattt
     1921 cttcgcgcgc attggcgccg gcgagctgca cgtggatctt ctgctggctg tccgccagcc
     1981 cgcgcagcat gtcgatggcg aagtaggaaa tacccgcggc gaccagcagc acgatggcca
     2041 cgttgctggc cgcgagccac cacccgaggc tgttgcgctt catcggcgcc ggtatcctgc
     2101 tcgcccttcg acgagcgtct tgatgatcag cgtggccacg gcgagcagcg acaacaccga
     2161 ggcgacggcg aatgcgccga ccatgttgta ttcgttgtac agcgactcga tttgcagcgg
     2221 catggtgttg gtcagaccgc gcacgttgcc ggagaccacg gacacggcgc cgaattcacc
     2281 catggcgcgc gcgttgcaca ggatgacgcc gtagatgagg ccccagcgaa tgcgcggcag
     2341 cgtaacacgg aagaaagtga accagccgcc ggcgccgagc gtgatcgccg cctcttcctc
     2401 gtcgttgccg agctgctgca tgagcgggat gagctcgcgc gcgacgtacg ggaaagtcac
     2461 gaacatcgtg gccagcacga tgccgggcaa ggcgtacacg atgctgatgt cgtgatcctg
     2521 cagccaggcg ccgaaccagc cgttgaggcc gaacagcaat acatagatga gtcccgaaac
     2581 caccggagac accgcgagcg gcaggtcgat cagcgtgatc aggaagctct tgccgcgaaa
     2641 ctcgaacttg gcgatacacc aggcggcggt gatgccgaac acggtattca gcgggacgac
     2701 gatcagggcc accgtcagcg tgaggcggat ggaagcgcgc gtgtcctcgt cggcgaaact
     2761 cgagaagtac gccgaccagc ccttctccag cgccatggcg aacacggccg ccagcggcgc
     2821 cgccagcagt gcgccgagga acgtcagcga gatggtgatc agcagccagc gcaccagcgc
     2881 cgaacgcagg ttggtgtgtc ccggccgggt agctagttcg gcggcgacgt cggcatggga
     2941 agaaggaccg gccatcagcg agacaccatc agtcgcttgc ggccccacac ctggatgagg
     3001 ttgatcgcga gcagcatcac gaacgagatc gccagcatca cgaagcccaa cgcggcggcg
     3061 ccggcgtagt cgtattcctc gaggcggaac acgatcagca gcggcgtgat ctcggtcttc
     3121 atgaacttgt tgccggcaat gaagatgacc gacccgtatt cacccacggc acgtgcgaag
     3181 gccagcgtga accccgtcag cagcgcggga agcacggtgg gaacgatgat gcgacggaat
     3241 acgtagaagc ggcccgcgcc gagcgtttcg gcagcgtcct cgagatcgcg ttgcacttcc
     3301 tgcaacgccg gctgcacggt gcgcacgacg aagggcatgc cgatgagcgt gagcgccacg
     3361 gtgatgccca gcggtgtgta ggcgacgaag atgccgcgtg gctcgaggta ctgaccgatc
     3421 cagccgttgg cggcgaagat ggtggtgagc gcgatgcccg acaccgccgt gggcagcgcg
     3481 aacggcatgt cgatgatggc gtcgaccagc ttgcggcccg ggaattcgta acgcacgagc
     3541 gtccacgcca gcgcgaaacc gaacaccatg ttcacgcagg ccgcgaccag cgaggcgccg
     3601 aacgacagct tgtacgacgc gagcgcacgc ggactcgcga tcacctcgac gaaacgcgac
     3661 cagtccatgc ccgcggtctt cagcaccagc gcggacagcg gcagcagcac gatggccgac
     3721 agaaagaatg tggtgaaccc cagc
//
LOCUS       DATDEU010000371         4009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2110465, whole genome shotgun sequence.
ACCESSION   DATDEU010000371 DATDEU010000000
VERSION     DATDEU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110465"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VIV63_08790"
     CDS             <1..1104
                     /locus_tag="VIV63_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY6124734.1"
                     /translation="KWLNITVIALSAVVGITGGVLLASKRDSKPVADTAPAAEGFLET
                     VAATASPPAAAPEGMVWIPGGEFSMGARDPMDMQDVVGMQATTDSRPIHRVAVEGFWM
                     DATEVTNEQFARFVKATGYVTVAERKPRQEDFPTAPPENLIPGSVVFHPPDHAVPLND
                     HFQWWAYVDGADWRHPLGPESSIAGKEKFPVVHIAYEDAEAYANWAGKRLPTEAEWEF
                     AARGGLSGKIYPWGDEFTKDGQWMANSHQGNFPNVDTGADAFVGIAAVAQYPANGYGV
                     YDVGGNVWEWTSDWYRPDYYVESAAAGVARNPTGPSDAFDPSEPGVPKKVHRGGSFLC
                     TDQYCSRYAVGTRGKGEVSTGTNHLGFRCVQGT"
     gene            1172..2464
                     /locus_tag="VIV63_08795"
     CDS             1172..2464
                     /locus_tag="VIV63_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY6124735.1"
                     /translation="MASKVVPISDRVVTGAAMDQPLPKRQRNLIVVAACVVLPVLVAA
                     GFWKLVPRGLRVAEMDLRIATVKQDLFRNDVVMRASAEPLHSVMLDAFQSGRVEEVLA
                     LDGAHVAQGDLLFRLSNPQLRLELVAREADRAQQISNLSMLRVAIETGETEHQRRLLD
                     VNFALTQARKQHARLVPLAKEGVVSAAALEESADRVARETQALADESTRFAVEAKIKR
                     DGAKQMERAIDRLDAGLKVVNESIDGLAVRAPIAGRLTDFHLQLGEIVKPDQHIGRID
                     QPGAVKLVAQVEEFFLDGMIAGKEGVASVNGRDFPVAISRVFPQVANGRFAIELLFVS
                     EAPKGMSPGQSAETRITLGETSPALILPNDSFINDTGGAWVFVLSPDGETATRRVIRT
                     GRRNNRQVEIAAGLEAGERVIVSNYGTFGKVERLQIAR"
     gene            2475..3134
                     /locus_tag="VIV63_08800"
     CDS             2475..3134
                     /locus_tag="VIV63_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6124736.1"
                     /translation="MLKFVNTTKKFVGGEVVTTALNGISLEIKAGEYVAVTGPSGCGK
                     TTLLSIIGMLDTPDGGEYWFDGQNVAGFREDQLNELRRGRIGFIFQSFNLIEELSVEE
                     NVDLALEYTDLPAKERSERVAQMLEKLGVAHRARHRPSQLSGGQQQRVAVARALVTQP
                     AVLLADEPTGNLDSAHGEEVMELLQKINDEGTTIVMVTHSPAHAARAKRTVGLLDGAI
                     V"
     gene            3131..>4009
                     /locus_tag="VIV63_08805"
     CDS             3131..>4009
                     /locus_tag="VIV63_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6124737.1"
                     /translation="MKRVSVLGLAVGLAAFLLLSGFVRYSWTYNAHVPDAEHVYLIKH
                     RFNLEGGRRWWDKAPMVLREAARATPGVTDATGYLGWFPLNIRVDGQLHKLRSLTVLP
                     GFAEMMGLKAVKGDLTEALTRPDAFAMTESAAIRLFGTADVVGRSFLLNSVETQTTAR
                     IAAILRDPPANTTIPFESLNGLNLALVPPLFRDEALRGEMGWPGHLLIRLHPEASVAA
                     VTEALQKFMDQAVLRLNLPPAAMASLGEAKVMDVQLLPIREAYFDRNVVVDAHTLGVD
                     RGDRNVVAGLMVIAVLI"
BASE COUNT          721 a         1216 c         1376 g          696 t
ORIGIN      
        1 aagtggttga acatcacggt cattgccctg tcggcagtgg ttggaattac cggaggcgtg
       61 cttctggcct cgaaacggga ctcgaagccg gtggcggata cggctccggc ggcggaaggg
      121 tttcttgaga cggtcgcggc caccgcgtcg ccgcccgcgg cggcgcccga aggcatggtg
      181 tggatcccgg gcggcgagtt ctcgatgggc gcgcgggatc ccatggacat gcaggacgtg
      241 gtcggcatgc aggcgaccac ggattcgcgg cccatccatc gcgtcgccgt cgaaggcttc
      301 tggatggacg cgaccgaagt caccaacgag cagttcgcgc gcttcgtgaa ggccaccggt
      361 tacgtgacag tggcggagcg caaaccgcga caggaagatt ttccgacggc gccgcccgag
      421 aacctgattc cgggttccgt ggtgtttcat ccgcccgatc atgccgtgcc gctcaacgat
      481 cacttccaat ggtgggcgta cgtcgatggc gcggactggc gccatccgct ggggccggaa
      541 agctcgatcg ccggcaagga aaaatttccc gtcgtgcaca tcgcctacga agacgcggaa
      601 gcgtatgcga actgggcggg caagcgtcta cctaccgaag ccgagtggga gttcgcggcg
      661 cgtggcgggc tctcggggaa gatctatcca tggggcgacg agttcacgaa ggatggccag
      721 tggatggcga acagccacca gggcaacttt cccaacgtcg ataccggcgc cgatgcgttc
      781 gtgggcatcg cggccgtcgc gcagtatccg gccaacggat atggcgtgta cgacgtgggc
      841 ggcaatgtgt gggagtggac cagcgactgg taccggcctg actactatgt cgaatcggcg
      901 gcggccggcg tcgcgcgaaa tccgacagga ccttcggacg cgttcgatcc gtccgagccc
      961 ggtgtgccga agaaggtgca tcgcggcggg tccttcttgt gcacggatca gtactgctcg
     1021 cgctacgcgg tcggcacgcg cggcaagggc gaagtgagca cgggcacgaa ccacctcggt
     1081 ttccgctgcg tgcaggggac gtgacgccaa agcttgcttt tcaaaaacta caagtgtcat
     1141 agtaacaacc acacgagtca tagggatgcc gatggcctcc aaggttgttc cgatcagcga
     1201 cagggtggtc acgggcgccg cgatggatca gccgctacca aagcggcagc gcaatctcat
     1261 cgtggtggcc gcgtgcgtgg tgttgcccgt cctggtggcg gccgggttct ggaagctggt
     1321 gccgcggggc ttacgcgtcg cggagatgga cctgcgcatc gccacggtga agcaggatct
     1381 gttccgcaac gacgtggtga tgcgcgcgtc cgccgagccg ctgcactccg tgatgctcga
     1441 cgcgttccag tccggccgcg tggaagaagt gctggcgctc gacggcgcac acgttgccca
     1501 gggcgatctt ctgttccgcc tgtccaaccc gcaactgcgg ctcgaactcg tggcgcgcga
     1561 agcggaccgc gcgcagcaga tctccaacct ttccatgttg cgtgtcgcga tcgaaaccgg
     1621 cgagaccgag catcagcggc gtctgctcga tgtgaatttc gcgctgacgc aggcgcgcaa
     1681 gcagcatgcg cggctcgtgc cgctcgcgaa ggaaggtgtg gtgtctgcgg cggctctcga
     1741 ggagtccgcg gaccgcgtgg cgcgcgagac ccaggcgctc gccgatgaga gcacccgctt
     1801 cgcggtcgaa gcgaagatca agcgcgacgg tgcgaaacag atggagcgcg ccatcgatcg
     1861 actggatgcg ggactcaagg tggtcaacga gtccatcgac ggactggccg tgcgtgcacc
     1921 tattgccggc cggctcacgg acttccacct gcagttaggc gagatcgtga agccggatca
     1981 acacatcggg cgcatcgatc agcccggcgc ggtgaagctg gtcgcgcagg tcgaagagtt
     2041 ctttctcgat ggaatgatcg cgggcaagga gggcgtcgcc agcgtgaatg ggcgcgactt
     2101 ccccgtcgcg atcagccgtg tattcccgca ggtcgccaac gggcgtttcg ccatcgagct
     2161 gctgttcgtg agcgaggccc cgaaaggcat gagcccgggg cagagcgccg aaacgcgcat
     2221 cacgctgggt gagaccagtc ccgcgctgat cctgcccaac gactcgttca tcaacgacac
     2281 cggcggcgcc tgggtgttcg tgctctcgcc cgatggcgag accgccacgc ggcgtgtcat
     2341 ccgcaccggc cgccgcaaca accgccaggt cgagattgcc gcggggctcg aggccggcga
     2401 gcgcgtcatc gtttccaact acggcacgtt cggcaaggtc gagcggctgc aaatcgcccg
     2461 ttaacgaggg acccatgctc aagttcgtca acaccaccaa gaagttcgtc ggcggcgaag
     2521 tcgtcacgac agcgctcaac ggcatcagtc tcgagatcaa ggcgggtgag tacgtcgccg
     2581 tcacgggtcc gtccggttgc ggcaagacca cgctgctgtc catcatcggc atgctcgata
     2641 cgccggatgg tggcgaatac tggttcgatg gccagaacgt ggcgggcttc cgcgaggacc
     2701 agctcaacga gcttcgacgc ggacgcatcg gcttcatctt ccagagtttc aatctcatcg
     2761 aagagctgtc ggtggaagag aacgtggatc tggcgctgga gtacaccgac ctgccggcga
     2821 aggagcgcag cgagcgtgtg gcgcagatgc tggagaaact cggcgtcgcg catcgcgcca
     2881 ggcatcggcc ctcgcaactc tcgggtggac agcagcaacg tgtcgcggtg gcgcgtgcgc
     2941 tggtaacgca gccggcggtg ttgttggccg atgagcctac gggcaacctc gactccgcgc
     3001 atggcgagga ggtcatggag ctcctgcaga agatcaacga tgagggcacg accatcgtca
     3061 tggtcacgca ctcgccggcg cacgcggcac gcgccaagcg gaccgtcggg ctgctcgacg
     3121 gggcaatcgt atgaagcggg tctcggtact gggactggcc gtgggactcg cggcgtttct
     3181 gttgctgtcg ggttttgtcc gctactcgtg gacctacaac gcgcatgtgc cggacgccga
     3241 acacgtgtac ctcatcaaac accgcttcaa tctcgaaggc ggccggcgat ggtgggacaa
     3301 ggcgccgatg gtgttgcgcg aggccgcgcg tgcgacacca ggagttaccg acgcgacggg
     3361 ttacctcggc tggtttccgc tcaacatccg ggtagatggg caactgcaca agctcagaag
     3421 tctgaccgtg ctgcccggct ttgccgagat gatgggattg aaggcggtca aaggcgatct
     3481 gaccgaagcc ctcacgcgcc cggatgcgtt cgcgatgacc gaatccgccg cgattcgcct
     3541 gttcggcacc gcggacgtcg tgggtcgctc gttcctgctg aactccgtgg agacccagac
     3601 gacggcgcgc atcgcggcga tcctgcgcga ccctccggca aacacgacga ttccgttcga
     3661 gagcctcaat ggcctgaacc tcgcgctcgt gccgcccctg tttcgtgacg aagcattgcg
     3721 aggcgagatg ggttggccgg ggcatctgct gattcgcctg catccggaag cgtcggtggc
     3781 ggcggtcacc gaagcgttac agaaattcat ggatcaggcg gtgctgcggc tcaacctgcc
     3841 gccggcggcc atggcgtcgc tgggtgaagc gaaggtgatg gatgtacagc tcctcccgat
     3901 tcgcgaggcc tatttcgatc gcaacgtggt cgtggatgcg catacgttgg gcgtggatcg
     3961 cggcgatcgc aacgtggtcg ccggtctcat ggtgatcgcg gtgctgatc
//
LOCUS       DATDEU010000372         4861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1964579, whole genome shotgun sequence.
ACCESSION   DATDEU010000372 DATDEU010000000
VERSION     DATDEU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964579"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VIV63_08810"
     CDS             <1..272
                     /locus_tag="VIV63_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124738.1"
                     /translation="GTAVEGVVGERNAENMAAYTRVDLRANRDVQLRNSRISFYLEVT
                     NLLNSRNECCVEHFHLEQRSAGPYLVKETAYWLPMLPSFGFQWEF"
     gene            complement(273..1559)
                     /locus_tag="VIV63_08815"
     CDS             complement(273..1559)
                     /locus_tag="VIV63_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124739.1"
                     /translation="MRGLAAWWAALLILSGARAFAHSSPQSIVKLDFLEHSIHAELLV
                     PESELAFATATDPAPFEAYLLRHVKAATPEGAPWKVEVRSVRNTSYLDHDYLQADVVM
                     TPPAGSPVRSLVLTDDAVTHEVRSHVIVVLAQTSAAPVMLGALQHPVRTLQIDQTGAQ
                     VWTPPAITSAEYESSPTFTPDGREMYFFRANARFGDYRLLVSRCVNGAWSAPEPPSFA
                     SKLPVLEADPFVSIDGKQLYFISSRHRQTRPGEEDLDIFVVNRGAHGAWGEPQRLPEP
                     VNSPGAELLPRLLADGRLLFGSSRDGGFGLGDIYIGTFTEGAWKVANAGPPISTPANE
                     YEAEMSRDGKRLVVVADRGDRSHLYLFRHGESGWAESGKVPARADVFQVGPLLSPRGD
                     RLLFAQAEGTRSGEIFLADLDVKPDTAWPPACASGQ"
     gene            complement(1559..2656)
                     /locus_tag="VIV63_08820"
     CDS             complement(1559..2656)
                     /locus_tag="VIV63_08820"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEY6124740.1"
                     /translation="MNRQWSVTAMCGLVFIAGGMAHAADTSCPGPSAGHGEVQAVVSA
                     IHALEKTLNTEQRASLERPFVLESAIRWSNLPVGIVPRAGLRLGDLDSKQAEAAHRVA
                     GAALSACGLKMLDEIRVADDLLKAVDERKIGWDGGNYFMSILGAPSARTPWMLQIGGH
                     HIAYNLTFNGGREGATPLFFGSEPISFSVAGVDYEPLATQSTAMSNLARALSKHPAAK
                     LSGTFTDVVKGVVVVSVPGQMPTGGTDTGFPLSFPTGKTDRGILYGALSADEQALVRT
                     AIESYASLPGAAITKPLVSAYESAEALADTYVGYSGQPDLSAENSYVRIDGPRIWMEL
                     AVQRAVADRTKLHYHALWRDKQSDYGGEIGH"
     gene            complement(2653..3075)
                     /locus_tag="VIV63_08825"
     CDS             complement(2653..3075)
                     /locus_tag="VIV63_08825"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEY6124741.1"
                     /translation="MRFLTALIAGLLFGAGLLVSGMTNPANVLGFLDLFGDWQPQLAF
                     VMGAAVLTAAPSFWAVRRRQRSLLGTPVELPDRRRIDKPLLVGATLFGVGWGLSGICP
                     GPGIMLLASGHGMAYLFMAAVVAGMWLVHLVRNKVGTR"
     gene            complement(3072..3482)
                     /locus_tag="VIV63_08830"
     CDS             complement(3072..3482)
                     /locus_tag="VIV63_08830"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY6124742.1"
                     /translation="MKSPEVLRAIAGGMLIGAGSATLLLLNGRVAGVSGILGNVIRES
                     FGEQGWRLAFLLGLLIPALFLGVSALHLPPHWLWAIASGLLVGAGTQIGSGCTSGHGV
                     CGLANLSTRSLVATLVFMSAAMVTVFIVRHAGPS"
     gene            3674..4195
                     /gene="ssb"
                     /locus_tag="VIV63_08835"
     CDS             3674..4195
                     /gene="ssb"
                     /locus_tag="VIV63_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY6124743.1"
                     /translation="MARGINKVILIGNLGADPETRAMPSGMTVANIRIATSENWKDKQ
                     SGENKERTEWHNVAMFGRLGEIAGEYLKKGSKVYIEGSLRTRKWQDKSGNDRYTTEII
                     ANEMQMLDSRGGGGMGGGGGGDYGGGGGGGGGGGGRGKQQQSPPDDYDQGGPPAGGGS
                     DKGGGEFDDDIPF"
     gene            4260..4754
                     /locus_tag="VIV63_08840"
     CDS             4260..4754
                     /locus_tag="VIV63_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6124744.1"
                     /translation="MSSRSGFDALVAEHQPMVARICASYERDRDLARELSQDVWFAIW
                     RALPGFRGDASLKTFVARIAQFRAISHVTQRAREPLQADLSEDIADLRPAPETAAIAS
                     SERIGLLAAVQRLPLVYRQPVILTLEDFSPAEIAAMLGLSVNVVGVRHTRARSMLRSL
                     LGAL"
BASE COUNT          927 a         1557 c         1568 g          809 t
ORIGIN      
        1 gcggcacggc cgtcgagggc gtcgtgggcg aacgaaatgc cgagaatatg gccgcctaca
       61 cgcgggtaga cctgcgtgcc aatcgcgacg tgcagcttcg caacagccgg atctcgttct
      121 atctcgaagt gaccaacctg ctcaacagcc ggaacgagtg ctgcgtcgag cactttcacc
      181 tggagcagcg cagtgccggt ccttatctcg tcaaggagac ggcctactgg ttgccgatgc
      241 tgccgtcgtt cggcttccag tgggagttct agctactggc cgctcgcgca ggcaggcggc
      301 catgcagtat ccggtttgac gtcgaggtcc gcgagaaaga tctcgccgga gcgtgtgcct
      361 tcggcctgtg cgaacaacag gcgatcaccc ctgggcgaca gcaacggtcc aacctggaat
      421 acgtccgcgc gggcagggac cttgccagat tctgcccagc ctgattcacc gtgccggaac
      481 agatagagat gggaacgatc gccacgatcc gcgaccacca ccagcctctt gccatcccga
      541 gacatctcgg cctcgtactc gttcgcggga gtgctgatcg gcggaccggc attcgcgact
      601 ttccacgcgc cctccgtgaa cgtaccgatg tagatgtcac cgagtccgaa gccgccgtcg
      661 cgactcgagc caaacagcaa ccgcccgtcg gccagcagcc gcggcagcag ttcggcgccc
      721 ggagaattca cgggctcggg cagccgttgc ggctcgcccc atgcgccatg ggcgccgcga
      781 ttcacgacga agatgtcgag atcctcttcg ccaggcctgg tctgcctgtg ccgcgacgag
      841 atgaaataga gttgtttgcc gtcgatggaa acgaacggat ctgcctccag caccggcagc
      901 ttcgacgcga acgagggcgg ctcgggagcg ctccatgcgc cattcacgca acgcgagacc
      961 agcaggcgat agtcgccgaa gcgcgcgttc gcgcgaaaga aatacatctc gcggccgtcc
     1021 ggcgtgaatg tcggcgacga ttcgtattcc gcgctggtga tggccggcgg ggtccacact
     1081 tgcgcgccgg tctggtctat ctgcagcgtc cgcaccggat gttgaagcgc gcccagcatg
     1141 acgggagcgg cggacgtctg tgcaaggacc acgatgacgt ggctgcggac ttcatgtgtc
     1201 acggcatcat cggtgagcac caggctgcgc accggacttc cggccggtgg cgtcatcacg
     1261 acatcggctt gtagatagtc atggtcgaga tacgacgtgt tgcgcaccga acgcacttcg
     1321 actttccacg gcgcgccctc gggcgttgct gctttcacgt ggcgtaacag atatgcctcg
     1381 aagggtgccg ggtccgtagc cgtggcgaat gcgagctcgg actcgggtac caggagctcc
     1441 gcgtgtatgg agtgctcgag gaaatcgagc ttgacgatcg attgcggtga gctgtgcgcg
     1501 aaggcgcggg cgccagacaa gatcagcagt gccgcccacc atgccgccaa tccgcgcatc
     1561 agtggccgat ctcgccgccg taatcggact gcttgtcgcg ccacaacgcg tggtagtgca
     1621 gctttgtgcg atccgccacc gcgcgttgca ccgccagctc catccagatg cgcggtccgt
     1681 cgatgcgcac gtacgagttc tccgcgctca ggtccggctg gccggaatag ccgacgtagg
     1741 tgtcggcgag cgcttccgcg gattcatacg ccgacaccag cggcttcgtg atggccgcgc
     1801 cgggtagtga ggcataactt tcgatcgccg tgcgcaccag cgcctgctcg tcggccgaca
     1861 gcgcgccata gagaatgccg cgatcggtct tgcccgtggg aaacgacagc gggaacccgg
     1921 tgtccgtgcc accggtcggc atctgccccg gaacggagac gacgaccacg cctttgacca
     1981 catcggtgaa cgtacccgac agttttgctg ccggatgttt cgagagcgca cgcgccagat
     2041 tcgacatggc cgtgctctgc gtggcgagcg gttcgtagtc gacaccggcc acggaaaagg
     2101 agatgggctc gctgccgaag aacagcggcg tcgcgccttc gcggccgccg ttgaacgtga
     2161 ggttgtaggc aatgtggtgc ccgccgatct gcaacatcca tggcgtgcgt gcggaagggg
     2221 cgccgaggat gctcatgaaa tagttgccgc cgtcccagcc gatcttgcgt tcgtcgaccg
     2281 ccttcaggag atcgtcggcg acccggatct cgtcgagcat cttgagcccg caggccgaca
     2341 gcgccgcgcc tgcgacgcgg tgcgcggctt cggcctgttt cgaatccagg tcgcccagac
     2401 gcaggcccgc gcgcggcacg atgcccactg gcaggttcga ccagcgaatc gccgactcga
     2461 gaacgaaggg gcgctcgagc gaagcgcgtt gttcggtatt gagcgttttt tcgagcgcgt
     2521 ggattgcgct cacgacggcc tgaacctcgc catggccggc ggaaggtccg gggcaggatg
     2581 tgtcggccgc gtgcgccatg ccgcccgcga tgaaaacgag tccacacatc gcggtcactg
     2641 accattgtcg attcatcgag tacccacctt gttgcgaacg agatgaacca gccacatccc
     2701 cgcgacgact gccgccatga acaaatatgc catcccatga ccgctggcga gcagcatgat
     2761 gccgggtccg gggcagatgc cactgagccc ccagcccacg ccgaacagcg tggcgcccac
     2821 cagcaacggt ttgtcgattc ggcgacggtc gggtaattcc accggcgtgc cgagcaggct
     2881 gcgctggcgg cgccgcacgg cccagaacga aggcgccgcg gtgagtacgg cggcgcccat
     2941 cacgaacgcg agttgcggtt gccaatcgcc gaagaggtcg aggaagccca gaacgttggc
     3001 ggggttcgtc atgccggaga ccagcagtcc cgcaccgaag agcagtccgg cgatcaatgc
     3061 cgtgagaaat ctcacgaggg acctgcgtgg cgcacgatga acaccgtgac catggccgcg
     3121 ctcatgaaga ccagcgtcgc caccagcgag cgggtagata gattcgccag accgcagacg
     3181 ccgtgaccgc tggtgcagcc gctgccgatt tgtgtgccag cgcccaccag caggcccgat
     3241 gcgatcgccc agagccagtg tggcgggaga tgcagcgcgc tgactccgag gaaaagcgcg
     3301 ggtatcagca ggcccagcag aaacgcgagc cgccacccct gttcgccgaa tgactcgcgg
     3361 atcacgttgc cgagaattcc actgacgccc gccacgcgcc cgttcagcag cagcaaggtc
     3421 gcggagcccg cgccgatgag catgccgccc gcgatggcgc gcaggacttc aggtgatttc
     3481 acgggagcgg caagtctttc gcatgatttt cgacaggtta cgcgcacaag ctcggagtaa
     3541 gaagcacgtt gccgccgaat tgattggtca agatttgtac agacacaatc gcggcagtta
     3601 cacgatctac atctgtgatg cctcagcccg tacaatcccg ccccacaata actttcgatg
     3661 aagggcattc gccatggcgc ggggaatcaa caaagtcatt ctgatcggca atctgggcgc
     3721 agacccggaa acccgcgcca tgccttcggg catgacggtg gcgaacatcc gcattgccac
     3781 cagcgaaaac tggaaagaca agcagtcggg cgaaaacaaa gagcgcaccg aatggcacaa
     3841 cgtcgccatg ttcggccgcc tcggtgaaat cgccggcgaa tacctgaaga agggttcgaa
     3901 ggtttacatc gaaggcagcc tgcgcacgcg caagtggcag gacaagtcgg gcaacgatcg
     3961 ctacaccact gaaatcatcg ccaacgagat gcagatgctc gattcccgcg gtggcggcgg
     4021 catgggtggc ggcggcggtg gagactacgg tggcggaggc ggcggtggcg gcggcggtgg
     4081 tggccgcggc aagcagcagc agtctccccc ggatgactac gaccagggcg gtccgcccgc
     4141 gggcggcggt tccgacaagg gcggcggcga gttcgacgac gacatcccgt tctaaccaga
     4201 ttccgcgcct gtaagtaaag gcgtgccacc gggactattg tctccgtgag tacgccttca
     4261 tgagttcccg ttccggtttc gacgcgctgg tcgccgagca ccagcccatg gtggcgcgca
     4321 tctgcgccag ctatgaacgc gaccgcgacc tcgcgcgcga gctctcccag gatgtgtggt
     4381 tcgccatctg gcgcgccttg cccggcttcc gcggtgacgc gtcgctcaag accttcgtgg
     4441 cgcgcatcgc gcagttccgc gccatctcgc atgtcacgca gcgcgcgagg gagccgctcc
     4501 aggcggatct gagcgaagac atcgccgatc tgcggcccgc tccggaaacc gcggcgatcg
     4561 catccagcga acgcatcgga ttgctcgccg cggtccagcg actgccactc gtgtaccggc
     4621 agcccgtcat cctcacgctc gaagattttt cgcccgccga aatcgctgcg atgctgggac
     4681 tcagcgtcaa cgtggtcggc gtccggcaca cgcgcgcacg ctcgatgctg cgctccctgt
     4741 taggtgcctt atgaacgagg agtggaaaga catgaagaac cagtggcagt cggggtccat
     4801 cgccgaaacg ttcatgaccg agaaacttcg ctggagcctg cgcctgcgca tgatcggctc
     4861 g
//
LOCUS       DATDEU010000373         8590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2110475, whole genome shotgun sequence.
ACCESSION   DATDEU010000373 DATDEU010000000
VERSION     DATDEU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110475"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VIV63_08845"
     CDS             complement(<1..317)
                     /locus_tag="VIV63_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124745.1"
                     /translation="MTGVSPIVDRFYPIGIPGKAWGAAERAQWLARQRKLRSYADEIV
                     SRIERLSTRFAVEEYGQLDYPPDHYRLLALRSREAAEDRPWVLVTGGVHGYETSGVHG
                     ALQ"
     gene            580..1365
                     /locus_tag="VIV63_08850"
     CDS             580..1365
                     /locus_tag="VIV63_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124746.1"
                     /translation="MQVMSRDGTRIAYDQWGQGPAVIVVNGALSDRSAGAELAQLLAP
                     HFTVYSYDRRGRGDSTDTQPYSVKREIEDIEALIDRAGGSAFVYGKSSGASLALQAAS
                     ALGDKVRKLAIYEPPFNEAPGAAQEWREFGAKIDSLLAANQRADAITHFMKFVGAPEE
                     VVAKMKASPAWPGMLAMAPTLAYDIAVLGDDRSVPVAIASKVKAVTLVMDGGASAGPM
                     PFMRPTADKIGTAIPGARRLVVEGQAHDVSAKVLAPILSKFFN"
     gene            complement(1432..3570)
                     /locus_tag="VIV63_08855"
     CDS             complement(1432..3570)
                     /locus_tag="VIV63_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY6124747.1"
                     /translation="MSLRRPSAAKWRGGLALCALLFASISAPSHAQLPTAQTVAGQIR
                     VGWNLGNTLEAQCGETAWGNPATTQALINSVRAAGFNAIRIPAAWNCHTNPAGSTTID
                     TAWMARVKTVVDYAYNQGMYVILNIHWDGGWLQDNPTFAFQQANNQKQAAFWTQIATT
                     FRTYNERMLFAGTNEVHADYGTPTSEHITVQQSYNQTFVNAVRATGGNNSSRTLVVQT
                     YNTNAWHGLNYFTMPTDSAASRLIVEIHHYDPYDYTINPSGPCLAWGTPYTQYSQCSW
                     AQENYHTDLFNQIRAKWVAAGVPVIIGEYGVARRSNANAASREYYLETVTRTAQNNGL
                     KMFYWDNGAQSGSSDVFALFDRNSGAIVDSGGLNAIMRGVGGTSTSYALSVTKSGSGA
                     GTVASSPAGINCGSTCNANFSSGTSVTLSATASSGSTFAGWSGACTGTASSCTVSMTA
                     ARTVTASFNSSAVNYTLSVTKSGSGTGTVTSNTGGINCGSTCSASFASGTSVTLTAAA
                     GSGASFAGWSGACSGTGACTVSMTQARSATATFNTASTFAASVTRAGTGSGTVTSNPG
                     GINCGTSCTANYASGTSVTLTAAAASGSTFAGWSGSCTGTGNCVLSMTASRAVTATFN
                     TSGGTGGTCANPVTFTNQTGNFNTTGAVCYRTSQNIAGWGCSNFDGRTLTVGGVARTC
                     GQLPLTRSADGYYYFSVTAGQYSWASLYAW"
     gene            3832..4602
                     /locus_tag="VIV63_08860"
     CDS             3832..4602
                     /locus_tag="VIV63_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6124748.1"
                     /translation="MRRVFITGSTDGLGRAAAQTLIGEGHQVVLHARSKQRASALADL
                     APRSAGVVIGDLASAAETRSLADQVNKIGRMDAVIHNAGIYREPNRAKTPEGHAKVFA
                     VNTLAPYMLTALIERPDRLIYLSSSMHFSGEASLRDIDWLERNWDTNQAYSDSKLQVT
                     ALAIALARRWPKVLSSAVDPGWVPTKMGGAGAPDDLEQGHLTQTWLAVSDEPAAKVSG
                     AYWHHRKQRKPAAGAIDPAFQDQLIAKLAEITGVALPP"
     gene            complement(4618..6096)
                     /locus_tag="VIV63_08865"
     CDS             complement(4618..6096)
                     /locus_tag="VIV63_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY6124749.1"
                     /translation="MSRSFTLPFLRSLFIVAVALCATSHVAAQTPVRIMPLGDSITAG
                     PGCWRAYLWNQLQTAGYSNIDFVGSVNDGGGCNPGFAYDFNHEGHSGFSITGIADANQ
                     LPPWLTAANPNIIVMHLGTNDMWGGWIPLETKITALTKLIGQMRANNPNIRIVMAQII
                     PMSPAGCTTCASDVVAFNNAIVPLAASLNTPQSPIYVVDQWTGFDLATDTYDAVHPLT
                     SGFIKMANRFFPAVAQALNSGAPVTSALSVSKAGTGSGTVTSTPAGIHCGAACTANFT
                     TGSSVTLSAAAASGSTFAGWSGACSGTGACTVTMAGARAVTATFNSSVTTFALSVTRA
                     GAGTGTVTSSPAGISCGAACSANYASGTSVTLTAAVASNSTFAGWSGACSGTGSCVLS
                     MTQARAVTATFNTSGGTGGTCTNPVTFSGNSGNFNTTGGVCYRTNANISGWGCYNFDG
                     RTLSVGGVARACGQMPLTRAADGYYYFAVTAGQFPWAGLFTW"
     gene            6527..7879
                     /locus_tag="VIV63_08870"
     CDS             6527..7879
                     /locus_tag="VIV63_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY6124750.1"
                     /translation="MVLAGTTTAMADVAPLSTRGNQILAGGQPASFAGNSLFWSNTGW
                     GGEKYYNASVVRWLKTDWKSNIVRAAMGVDDSGGYLQDPSGNLARVRTVVDAAIANDM
                     YVIIDWHSHHAESYRSNAISFFQDMARTYGTRNHVIYEIYNEPLQISWSGQIKPYAQA
                     VINAIRAIDPDNLIVVGTPTWSQDVDVAAADPVSGINIAYTLHFYAGTHHQFLRDKAQ
                     TALNRGAPLFVTEWGAVNADGNGAVNTGETNTWMSFLKSRGISHANWATNDKAEGASA
                     LVPGASTAGGWPANQLTTSGTLVKQIISTWGPTSPPPPPPPGGTVISTIQAEAFSQMS
                     GIQTQTTTDTGGGLNVGWIDVNDWLSYMNSAVSIPSTGSYRIEYRVASLNGGGGLRLE
                     EAGGSPVHGQLAVPSTGGWQTWTTISHTINLSAGTHRFGINAISGGWNLNWFRVVRLN
                     "
     gene            complement(7920..8231)
                     /locus_tag="VIV63_08875"
     CDS             complement(7920..8231)
                     /locus_tag="VIV63_08875"
                     /inference="COORDINATES: protein motif:HMM:NF023232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3325 domain-containing protein"
                     /protein_id="HEY6124751.1"
                     /translation="MKLFLALLLSFAGLALFALAMERHWRQLRATQPLTAATSRTLRI
                     AGVVALISSLVLCLSVDHASMASLVWIMTMAVSAFVLAMLLAWRPAWLSPLIRWVRVK
                     S"
     gene            complement(8236..>8590)
                     /locus_tag="VIV63_08880"
     CDS             complement(8236..>8590)
                     /locus_tag="VIV63_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124752.1"
                     /translation="NRLLPEDLVDRDEWQKNVFWLTWLASGIHAFWRTREVLLAEAAP
                     AWREQTLVVAVLAFAAPVLNWVTTGDHLLATLKAEYWPVAGIDLFLLTTGVIASLIAI
                     RLRRPALVHTRLATN"
BASE COUNT         1613 a         2708 c         2743 g         1526 t
ORIGIN      
        1 aactgcagtg cgccgtgcac gccgctcgtc tcgtagccgt gcacgccgcc cgtgaccagc
       61 acccacggtc gatcttcggc tgcctcgcgg ctgcgcaacg ccagtagccg atagtggtcc
      121 ggtggatagt cgagttggcc gtactcttcc accgcgaagc gtgtgctgag cctctcgatg
      181 cggctcacga tctcatcggc gtagcttcgc aacttgcgct gtcgcgcgag ccactgcgcg
      241 cgctccgctg caccccacgc tttgccggga atgccgatgg gataaaaacg gtcgacgatt
      301 ggcgataccc cggtcatcat gttcctcgtg gactaactaa cacagtgcgt gatcgtcccg
      361 aaagcggccc actcgacgac ggccgcgtgc tcttcctacg gacagcgttc cattctgact
      421 acgggcctcg aaacgcgatt ctcgtataaa tcggtatcgc ctcaaaaccg gagcggcgtc
      481 atgccaacga tatctcaatc gtctctgttt cgatttgcct gtctggccgc actcctgtcg
      541 tttgcgtcgc cagcgacgag ccaggctgag ggaaaaccga tgcaggtcat gtcgcgagac
      601 gggactcgaa ttgcgtacga ccagtggggc cagggccctg cggtcatcgt ggtgaacggc
      661 gcattgtccg atcgatcggc tggcgccgaa ctcgctcagc tcctcgcgcc tcacttcacg
      721 gtgtacagct acgatcgtcg aggccgtggc gacagtaccg acacccaacc ctattcggtg
      781 aagcgcgaga tcgaagacat cgaggcactc atcgaccgcg ccggcggctc agctttcgtg
      841 tacggcaaat cttcgggggc gagcctcgca ctgcaggccg cgtcggcgct cggcgataag
      901 gtcagaaagc tggccattta cgaaccgcct ttcaacgaag ccccgggcgc cgcacaagag
      961 tggcgagagt tcggcgcgaa gatcgacagc ctgctcgctg cgaatcaacg agccgacgcc
     1021 atcacgcatt tcatgaaatt cgtcggcgcg cccgaagaag tcgtggcgaa aatgaaagcg
     1081 tcgccggcgt ggccgggaat gctggcgatg gcgccaacgc ttgcctatga catcgccgtc
     1141 ctgggggacg accgttccgt gccagtcgcg attgcgtcca aggtgaaagc ggtcacgctg
     1201 gtcatggacg gtggcgcaag cgctggccct atgcctttca tgcgtccgac agcggacaag
     1261 attggcacgg cgatcccggg cgcccggcgt ctcgtggtcg agggccaggc ccatgatgtc
     1321 agtgcgaaag ttttggctcc catcctgtcg aagttcttca actgagcccg tgcaaggcgc
     1381 gatttaccgg cccgccccta cagggacggg ccggtcatca gttagccact cctaccaggc
     1441 gtacaaactc gcccaggaat actggcccgc ggttaccgag aagtagtagt aaccgtctgc
     1501 cgaccgcgtg agcggcagtt gtccgcaggt gcgcgccact ccacccaccg tcagcgttcg
     1561 tccgtcgaag ttcgagcaac cccacccggc gatgttctgg ctggtccggt aacagaccgc
     1621 gcccgtggtg ttgaaattgc ccgtttggtt ggtgaacgtg accggattgg cgcaggtgcc
     1681 gccggttccg ccgctcgtgt tgaatgtcgc ggtgactgcg cgcgaggccg tcatcgacaa
     1741 cacgcagttg cccgtgcccg tgcacgaacc actccagccc gcgaatgtcg atccgctcgc
     1801 cgctgcggcc gtcaaggtca cggacgtgcc gctggcgtag ttagccgtgc acgacgtgcc
     1861 gcagttgatg ccgcccggat tcgaagtcac ggtgccgctg ccggtgcccg cgcgcgtgac
     1921 gctcgccgcg aacgtggacg cggtgttgaa cgtcgccgtc gccgaacgcg cctgcgtcat
     1981 cgacaccgtg caggcacccg tgcctgaaca cgcaccgctc cagccggcga agctcgcgcc
     2041 gctgcccgcc gccgcggtca gcgtgacgga agtgccactg gcgaaactcg cactgcaggt
     2101 gctgccgcag ttgatgccgc cggtgttcga cgtcaccgtg ccagtcccgc tgcccgattt
     2161 cgtcacgctc agcgtgtagt tcacggccga actgttgaac gaggccgtaa ccgtgcgcgc
     2221 cgcggtcatc gacaccgtgc agctggaggc cgtgccggtg cacgcgccgc tccagcccgc
     2281 gaacgtcgag ccgctcgacg ccgtagcgga caacgtgacg gaagtgccgc tgctgaagtt
     2341 cgcgttgcag gtcgatccgc agttgatgcc cgcgggcgac gaggcgaccg tgccggcgcc
     2401 gcttccgctc ttcgtcacgg acagcgcata cgacgtgctg gtgccgccca cgccacgcat
     2461 gatcgcgttc agtccacctg aatcgacgat cgcaccgctg ttgcggtcga acagcgcgaa
     2521 gacatcgctg gaacccgact gtgcgccgtt gtcccagtag aacatcttga ggccgttgtt
     2581 ctgggcggtg cgcgtgacgg tttcgaggta gtactcgcgc gaggcggcgt tggcattcga
     2641 acggcgcgcc acaccgtact cgccgatgat caccggaact ccagcggcca cccacttcgc
     2701 gcggatctgg ttgaacaggt ccgtgtggta gttctcctgc gcccacgagc actggctgta
     2761 ctgcgtgtac ggagtgcccc acgccaggca cgggccgctc ggattgatcg tgtaatcgta
     2821 aggatcgtag tgatggatct cgacgatcaa ccggctcgcg gccgaatccg tgggcatggt
     2881 gaagtagttg aggccgtgcc acgcgttcgt gttgtaggtc tgtaccacca gcgtgcgcga
     2941 agagttattg ccaccggtcg cgcgcacggc attcacaaac gtctggttgt acgactgctg
     3001 caccgtgatg tgctcggaag tcggcgtgcc atagtcggcg tgaacttcat tggtacccgc
     3061 gaacagcatg cgttcgttgt aggtacggaa cgtcgtcgca atctgcgtcc agaacgcggc
     3121 ctgcttctgg ttgttagcct gctgaaaagc gaacgtcggg ttgtcctgca gccagccgcc
     3181 atcccaatgg atgttcagga tgacgtacat gccctggttg taggcgtagt cgacgactgt
     3241 cttgacgcgt gccatccagg cggtgtcgat cgtcgtcgag cccgccgggt tggtgtgaca
     3301 gttccaggcc gcaggaatgc ggatggcatt gaaacccgcg gcgcgcacgc tgttgatcag
     3361 cgcctgcgtc gtcgccgggt tgccccacgc ggtttcaccg cattgggctt cgagtgtatt
     3421 accaagattc cagccaaccc ggatctggcc ggcaacggtc tgcgcggtcg ggagttgggc
     3481 gtgggacgga gcggaaatgg acgcgaagag caacgcgcaa agcgcgagcc caccgcgcca
     3541 tttcgcagcg gaaggtctgc gaagtgacat gggttccccc tgagttgatc aatccatcga
     3601 tcgtgtctgg cagcagggta gagcccccac tcgatccgct cgccgacggc cgattctatg
     3661 cgatgacacc ggtgtacatg caattcgggt tttgcgagat gaaccggagc ggtagtcttg
     3721 ctgtccgatt gattcgaggt gtctccaatg actcaatcaa tgtctcgacg ccgggttctg
     3781 caactggccg cgatggcggt cgccacatcg gcgactgcaa aggaacgatc catgcgacgc
     3841 gtattcatca cgggttcgac tgatggtctc ggacgcgccg cggcgcagac actcatcggc
     3901 gaaggccacc aggtcgtcct gcatgcgcgc tcgaagcaac gcgcttctgc attggctgat
     3961 ctggcgccgc gatccgcggg cgtggtcatt ggcgatctcg cgagcgccgc cgagacgcgt
     4021 tcgctggcgg accaggtcaa caagatcggc cgcatggacg ccgtgattca caacgccggc
     4081 atctatcgcg agccgaaccg cgcaaagacg cccgagggcc acgcgaaagt ttttgccgtg
     4141 aacacgctcg cgccctacat gctcaccgcg ctcatcgagc gtcccgatcg cctgatctac
     4201 ctcagcagca gcatgcactt cagtggcgaa gcgtcattgc gcgacatcga ctggctcgag
     4261 cgcaattggg acacgaacca ggcgtactcg gacagcaagc tgcaggtcac cgcgttggcg
     4321 atcgcactcg ccaggcgctg gccgaaagtg ctcagcagcg ccgtcgatcc gggctgggtg
     4381 ccgacgaaga tgggcggggc cggtgcgccc gatgatctcg agcagggaca tctcacgcaa
     4441 acctggctcg ccgtcagtga cgagcccgcg gcgaaagtca gcggtgcgta ctggcaccac
     4501 cgtaaacaac gcaaacctgc cgcgggcgcc attgacccgg cgttccagga ccagctcatc
     4561 gcgaaactcg ccgaaatcac gggggtcgcg cttcccccgt aatttgttag ttcctgccta
     4621 ccaggtgaaa aggcccgccc acgggaactg gcccgcggtg accgcgaagt agtagtaccc
     4681 atccgccgcg cgcgtcagcg gcatctgccc gcaggcgcgg gcgacgccac ccaccgacaa
     4741 cgtacggcca tcgaagttgt agcagcccca gccactgatg ttcgcgttgg tgcgataaca
     4801 cacgccgccc gtggtgttga agttgcccga gttgccggag aacgtgacgg ggttggtgca
     4861 cgtgccaccc gtgccgccgc tggtgttgaa cgtcgcggtc accgcgcgcg cctgcgtcat
     4921 ggacaacacg caactcccgg tgccgctgca tgcgccgctc cagccggcaa acgtggagtt
     4981 gctggcgacg gccgccgtga gcgtcaccga cgtaccgctt gcgtagttag ccgagcatgc
     5041 ggcgccgcag ctgatgccgg ccgggctcga tgtcacggtt cccgtgccgg cgcccgcgcg
     5101 cgtgacactg agcgcgaacg tcgtcaccga actgttgaac gtcgccgtga ccgcacgcgc
     5161 tcccgccatc gtcacagtgc aagcgccggt gcctgagcag gcgccgctcc agcccgcgaa
     5221 ggtcgagccg ctggcggctg cggcggacag tgtcaccgac gatcccgtgg tgaagtttgc
     5281 ggtgcaggct gcgccgcaat ggatccccgc gggagttgaa gtaacggtgc cgctgccggt
     5341 gccggccttc gacacggaca gggcgctcgt caccggcgcg cctgagttca gcgcctgtgc
     5401 gacggccgga aaaaaccggt tcgccatctt gatgaagccg gaggtcagcg gatgaacggc
     5461 gtcgtacgtg tcggtcgcga ggtcgaagcc ggtccactgg tcgacgacat agatgggtga
     5521 ctgcggggta ttgagactcg ccgccagcgg cacgatggcg ttgttgaacg ccacgacatc
     5581 cgaagcgcag gtggtgcagc cggccgggct catcgggatg atctgcgcca tgacgatgcg
     5641 gatgttcggg ttgttagccc gcatctggcc gatgagcttg gtcaacgcgg tgatcttggt
     5701 ttcgagcggt atccacccgc cccacatgtc gttggttccg aggtgcatga cgatgatgtt
     5761 gggattggct gccgtcagcc acggcggcag ctggtttgca tcggcaatgc cggtgatgga
     5821 gaaaccgctg tggccttcgt ggttgaagtc gtacgcaaac ccggggttgc agccgccgcc
     5881 gtcgttcacg ctgccgacga agtcgatgtt cgagtagcca gccgtctgca gctggttcca
     5941 cagatacgcg cgccagcagc ccgggcccgc ggtgatggag tcgccgagcg gcatgattcg
     6001 cacgggtgtc tgcgccgcca cgtgggaagt ggcgcagagg gcgaccgcga cgatgaacaa
     6061 cgagcggaga aagggcaggg tgaagctgcg ggacatgaga cctccttgtg atcgaaccgg
     6121 ccttgcgctg gccggcatct gcgcgggcat cttgcccacg aaccccggcg atcatgatcc
     6181 tgcgagtttc agatggaaag gaagatttct tcggcgagac gactcccctg ccgcgcgaac
     6241 gatcccgcgc tgcgcaacgg ccgccattta gttaggcacg ttgtccgtaa gccggaacac
     6301 gagacaaaag agacgacaag ccatccgatc cgcagacaat cgggacatcg ctttccgtgc
     6361 gcatgaaatc cgcgagcaca tttccggttg ttacgttggc gctcttgctg gcctgtgtta
     6421 gtagtcgcga cgcgccagtt ggcgctgcaa atcacggacg tgggggacaa tcatggatcg
     6481 agcaatagtc agggccgccg ggagactggc ggcaatatca gcactgatgg tgctggccgg
     6541 gaccaccacc gccatggcgg acgtggcgcc gctttcaacc aggggcaatc agattctcgc
     6601 aggaggtcag ccggccagct tcgccggcaa tagtctgttc tggagcaaca ccggctgggg
     6661 cggtgaaaag tattacaacg ccagcgtcgt tcgctggctg aagaccgatt ggaagtcgaa
     6721 catcgttcgc gcagcgatgg gcgtggacga tagtggcggg tatctccagg atccgtccgg
     6781 caatctggcg cgggtcagga cggtcgtgga cgcggccatc gccaacgaca tgtacgtgat
     6841 catcgactgg cattcgcatc atgccgagtc gtaccgcagc aacgccatct cgttctttca
     6901 ggacatggcg cgcacctacg gcacgcgcaa tcacgtgatc tacgagatct acaacgagcc
     6961 cctgcagatt tcctggagtg gccagatcaa gccgtacgcg caggcagtga tcaacgcgat
     7021 acgcgccatc gatcctgaca acctcatcgt cgtcggcact cccacctggt cgcaggacgt
     7081 ggacgttgcc gcagcggacc cggtgtcggg aatcaatatc gcgtacacgc tgcacttcta
     7141 cgcaggaact caccatcagt tcctgcgcga caaagcgcag acggcactga accgcggcgc
     7201 ccccctgttc gtcacggaat ggggtgccgt caacgcggat ggcaacggcg ccgtcaatac
     7261 cggtgagacg aacacctgga tgagtttcct caagtcgcga ggtatcagcc acgccaattg
     7321 ggcgaccaat gacaaggcgg aaggggcatc ggcgctcgtc cccggtgcga gtaccgcggg
     7381 cggatggccg gccaatcaac taactacgtc cggcacactg gtcaaacaga tcatttcgac
     7441 ctggggtccc acctcgccac cacctccgcc accaccgggc ggcacggtga tctcgaccat
     7501 tcaggctgaa gccttcagtc agatgagtgg cattcaaacg cagaccacca ccgacacggg
     7561 tggcggactc aacgtgggct ggatcgacgt caacgactgg ctgtcgtaca tgaattccgc
     7621 cgtgagcatt ccgtcgactg gcagctaccg catcgaatat cgcgtggcga gcctcaacgg
     7681 aggagggggc ttgaggctcg aggaagcggg cggtagtccg gtccatgggc aactcgccgt
     7741 tccctcaacc ggtggatggc agacctggac gaccatctcg cacaccatca acttgtcggc
     7801 cggaacgcat cgattcggca tcaatgcgat ctccggaggc tggaacctca attggtttcg
     7861 tgttgtacgt ttgaactgag aaaaacggcg agccggagtg cggcgctccg gctcgccgtc
     7921 taacttttca cgcgtaccca gcggatgagg ggcgagagcc aggcgggacg ccaggccagc
     7981 aacatcgcca atacgaaggc cgacaccgcc atcgtcatga tccagaccag cgacgccatc
     8041 gacgcatgat cgacgctgag acacagcacg agcgacgaaa tcagcgcgac gactcccgcg
     8101 attcgcaggg tgcgtgaggt cgccgcggtc aatggctgcg ttgcgcgcaa ctggcgccag
     8161 tgacgttcca tcgccagcgc aaaaagcgca aggccggcga atgaaagcag caatgccagg
     8221 aacaacttca tgaagtcagt tcgtcgcgag acgtgtgtgg acgagcgccg ggcgtcgcag
     8281 acggattgca atcaggctcg cgatgactcc cgtcgtgagc aggaagaggt cgatgccggc
     8341 gaccggccag tactcggctt tcagtgtcgc gagcaggtga tcgcccgtcg tgacccagtt
     8401 gaggacgggc gcggcgaacg cgagcaccgc gactaccagc gtctgctcac gccacgcagg
     8461 tgctgcttcg gctagcagca cttcgcgcgt gcgccagaag gcgtgaatgc ccgacgcgag
     8521 ccaggtcagc cagaacacgt tcttctgcca ttcatcgcgg tcgacgagat cctcaggcaa
     8581 caaacggttg
//
LOCUS       DATDEU010000374         3464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1090676, whole genome shotgun sequence.
ACCESSION   DATDEU010000374 DATDEU010000000
VERSION     DATDEU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090676"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VIV63_08885"
     CDS             complement(<1..706)
                     /locus_tag="VIV63_08885"
                     /inference="COORDINATES: protein motif:HMM:NF038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HEY6124753.1"
                     /translation="MLRPRVIPCLLIKNGGLVKTVKFGAPKYVGDPINAVKIFNEKEV
                     DELIVVDIDATAAGAEPNYERIRNLAAECRMPLCYGGGITTAAQAGRIIGMGVEKVAI
                     SAAAVATPQLIDELATTIGAQSVVVVLDVRKDPKRGTYEVWTHNGTRATGRSPVDLAR
                     EMEQRGAGEIVINSIDMDGVMKGYDLPLISAVREAVTLPLTALGGAGSLKDIQSLVAQ
                     MGIIGAAAGSLFVFKGT"
     gene            complement(711..1325)
                     /gene="hisH"
                     /locus_tag="VIV63_08890"
     CDS             complement(711..1325)
                     /gene="hisH"
                     /locus_tag="VIV63_08890"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY6124754.1"
                     /translation="MIAIVDYGLGNVHAIANIYKRLNIPATLADQPDKLRDASHIVLP
                     GVGAFDWAMSQLDGSGMRGALEDAVRDKRTPVLGICVGMQMLAKRSDEGQLDGLGWID
                     ADVRKFDVTRMTTRTRLPHMGWNDVRPRAGSPLFAGMEHDATFYFLHSYYFAPRDPAD
                     VLAQTDYHGEFACAVQAGNVYGVQFHPEKSHRWGISLLKNFAQV"
     gene            complement(1322..2461)
                     /locus_tag="VIV63_08895"
     CDS             complement(1322..2461)
                     /locus_tag="VIV63_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HEY6124755.1"
                     /translation="MQTTAYQICATCVMDTSDSKITFDAQGVCDHCRTFRSEIAPNWH
                     TDARGRADLDRIVEEIRKAGKGRDFDCIIGMSGGIDSSYLTCIAKEQLGLRPLVFHVD
                     AGWNSQIAVNNIEKLIDKLGLDLYTEVIDWEEMRDLQLAFFKSGVPHVDTPQDHSFFA
                     TMYNFAEKHDVRYILTGSNYSTECVRNPMEWMYYQSDSIQLRDIHKKFGTRPLHNFPI
                     TSILRHKVYLRYVKRIQVVAPLNYVPYPKEEAMRVLEQRFGWQPYPQKHFESRFTRFY
                     EAYWLPTRFGYDTRRVQFSSLILTGQMTRDQALEKLRHPAYDPETIAHDFEYVATKLG
                     IGVPELQSYLEAPKRTYRDFKSQQAIYLLGARVMRLLGLQLGGKR"
     gene            2610..>3464
                     /locus_tag="VIV63_08900"
     CDS             2610..>3464
                     /locus_tag="VIV63_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6124756.1"
                     /translation="MNSRSADFGTCRLCGSAANEVALKLSGMPRWNHRLLKDSEVATD
                     RAVDLEIHRCASCGFVSLPLQLQDDYYDDYVNAPSASPQMQQFQREQAKLFVERFGLR
                     GKSVLEVGCGDGFFLDMLRAEGAECTGIEPSEAQRAIAQARQLRVEGGLLTADRRLSA
                     GPFDAFATRQVFEHVPDLAGFLAAIRANLKPAAVGLVEVPNLDALIDESRFFDFIPEH
                     LNYFSPSSLRLVLELAGFEVLEVMPVQAGESLRALVRLIAPRTLAGIGTRVQELREAM
                     SRFVAESKA"
BASE COUNT          583 a         1081 c         1137 g          663 t
ORIGIN      
        1 acgtgccctt gaacacgaac aggctgccgg cggcggcgcc aatgataccc atctgcgcca
       61 ccagcgactg aatgtccttg agcgaaccgg cgccgccgag cgcggtcagc ggcagtgtca
      121 cggcttcgcg cacggcggag atcagcggca ggtcgtaacc cttcatgact ccgtccatgt
      181 cgatggagtt gatgacgatc tctccggcgc cgcgttgttc catctcgcgc gcgaggtcga
      241 cgggcgagcg gcccgtggcg cgcgtgccgt tgtgcgtcca cacctcgtac gtgccgcgct
      301 tcgggtcctt cctgacgtcg agcaccacga cgacgctttg cgcgccgatc gtcgtcgcca
      361 gttcgtcgat gagctgcggc gtggcgacgg ccgcggcgct gatcgcgacc ttctcgacgc
      421 ccatgccgat gatgcggccc gcctgggcag cggtggtgat gccaccgccg taacacagcg
      481 gcatgcggca ctcggccgcg aggttgcgga tgcgctcgta gttaggctcg gcgccggccg
      541 cggtcgcatc gatgtcgacc acgatgagtt cgtcgacttc cttctcattg aagatcttca
      601 cggcgttgat cgggtcgccg acatacttgg gcgcgccgaa cttcacggtc ttcacgagac
      661 cgccgttctt gatcagcaag cagggaatga ctcggggacg cagcatgaat tcagacctgc
      721 gcgaaattct tgagcagcga gattccccag cgatggctct tctcggggtg gaactgcacg
      781 ccgtacacgt tgcccgcctg gacggcgcag gcgaactcgc cgtggtagtc ggtctgcgcg
      841 agcacgtcgg cgggatcgcg cggcgcgaaa tagtaggagt gcaggaagta gaacgtggcg
      901 tcgtgctcca tgccggcgaa cagcggcgaa ccggcgcgcg gccgcacgtc gttccagccc
      961 atgtgcggca gccgggtgcg cgtggtcatg cgcgtgacgt cgaacttgcg aacgtcggcg
     1021 tcgatccagc cgaggccgtc gagctgccct tcgtcgctgc gcttcgccag catctgcatg
     1081 ccgacgcaga tgccgagcac cggcgtgcgc ttgtcgcgca ccgcgtcttc gagcgcgcca
     1141 cgcatacccg aaccgtcgag ctggctcatc gcccagtcga aggcgccgac tccgggaagc
     1201 acgatgtgcg aagcgtcgcg cagcttgtcg ggctggtcgg cgagcgtcgc gggaatgttc
     1261 aggcgcttgt agatgttggc gatggcgtgg acgttgccca ggccgtaatc gacgatcgcg
     1321 atcatctctt gccaccgagc tgcaggccga gcagccgcat cacgcgggcg ccgagcaggt
     1381 agatggcctg ctgcgatttg aaatcacgat aggtccgctt cggcgcttcg aggtaggact
     1441 gcagctccgg cacgccgatg ccgagcttcg tcgccacgta ttcgaaatcg tgcgcgatgg
     1501 tctcggggtc gtacgccggg tggcgcagtt tttcgagcgc ctggtcgcgc gtcatctggc
     1561 ccgtcaggat caggctcgag aactgcacgc ggcgcgtgtc gtatccgaaa cgcgtcggca
     1621 gccagtacgc ctcgtagaaa cgcgtgaagc gcgactcgaa atgtttctgc ggatagggct
     1681 gccagccgaa tcgctgctcg aggacgcgca tcgcctcctc tttcgggtac ggcacgtagt
     1741 tgagcggcgc caccacctgg atgcgcttga cgtaccgcag gtacaccttg tgacgcagga
     1801 tgctggtgat cggaaagttg tgcagcggac gcgtgccgaa tttcttgtgg atgtcgcgaa
     1861 gctggatgga atccgactgg tagtacatcc attccatcgg gttgcgcaca cactcggtgg
     1921 agtagttcga ccccgtgagg atgtagcgga cgtcgtgttt ctccgcgaag ttgtacatgg
     1981 tcgcgaagaa cgagtggtcc tgcggcgtgt cgacgtgcgg cacgcccgac ttgaagaacg
     2041 cgagctgcag atcgcgcatc tcttcccagt cgatgacctc ggtgtacaga tcgaggccga
     2101 gcttgtcgat gagcttttcg atgttgttga ccgcgatctg ggagttccag ccggcgtcca
     2161 cgtggaacac gagcggccgc aggcctaact gttccttggc gatgcacgtg aggtaggagc
     2221 tgtcgatgcc accgctcatg ccgatgatgc agtcgaaatc gcggcctttg ccggccttgc
     2281 ggatttcctc gacgatgcga tccagatcgg cgcggccccg cgcgtcggta tgccagttag
     2341 gggcgatttc cgaacggaaa gtccggcaat ggtcgcaaac gccctgggcg tcgaaggtga
     2401 tcttcgagtc gctggtgtcc atgacgcagg tagcgcatat ctggtaagca gttgtttgca
     2461 ttggtatttg tggcggagat gctcgcgaag cgggcgcatt ctatcgcagg taaaccgcca
     2521 cgagggccag gcaaaccgcg attccgtccg aatatttcac ctgcgcccgc gtgacacaat
     2581 cgcacccttt ttcaaacctg ggatgtggaa tgaattcgcg atcagccgat ttcggaacct
     2641 gccggctctg cgggtcggcc gccaacgaag tggcgctcaa actgtccggg atgccccggt
     2701 ggaatcaccg gctgctcaag gactcggaag tcgcgaccga tcgcgccgtc gatctcgaaa
     2761 tccatcgctg cgccagctgc ggcttcgtga gcctgccgct gcagctgcag gacgactatt
     2821 acgacgacta cgtcaacgca ccgtccgcgt cgccgcagat gcaacagttc cagcgtgaac
     2881 aggcgaaact cttcgtcgag cgctttggac ttcgcggaaa gtccgtgctc gaggtgggct
     2941 gcggtgacgg tttcttcctg gacatgctgc gcgccgaggg cgccgagtgc accggcatcg
     3001 aaccttcgga agcgcagcgc gcgatcgcgc aggcccgcca attacgcgtc gaaggcggcc
     3061 tgctcacggc cgaccgccgc ctgtctgccg gcccgttcga cgcgttcgcc acgcgccagg
     3121 tgttcgagca cgtgccggac ctcgcgggct tcctggccgc gattcgcgcc aatctcaaac
     3181 ccgccgccgt gggtctcgtc gaggttccga acctcgacgc cctgatcgac gaaagccggt
     3241 tcttcgattt cattcccgag catctcaact acttctcgcc ctcttccctg agactggtac
     3301 tggagctcgc gggattcgaa gtgctcgaag tgatgccggt ccaggccggc gaatcactgc
     3361 gcgcgctggt gcggttgatc gctccgcgga cgctcgcggg catcggcacc cgcgtgcagg
     3421 agctgcgcga ggccatgtcg cgcttcgttg cggagagcaa ggcg
//
LOCUS       DATDEU010000375         3886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1381757, whole genome shotgun sequence.
ACCESSION   DATDEU010000375 DATDEU010000000
VERSION     DATDEU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381757"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VIV63_08905"
     CDS             <1..651
                     /locus_tag="VIV63_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY6124757.1"
                     /translation="EYVHQHKGCVITHREVGVDTDNWHTALKNTLRQAPDVILIGEIR
                     DRETMEYGIQFAETGHLVLATLHANSSNQALDRIINFFPDERREQLLMDLSLNIRALV
                     SQRLVPREAGAGRIAAMEIMLNSPLIQDLIFKGKVQEIKEVMSRSNRVGMKTFDQALY
                     DLYEQGFISYEDALRNADSKNELRLRIKLESKREVKNLDDGGTLSMVDEEQQGRSF"
     gene            689..1807
                     /locus_tag="VIV63_08910"
     CDS             689..1807
                     /locus_tag="VIV63_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY6124758.1"
                     /translation="MNEKFVDTIEVKQASEGAPMVLNTRPLFKLMVERKASDLFFTSN
                     SPIKVKIEGAILPINKQVLTPETVRQAAYGLMTPDQLESFKRELEIDFAISEPGLGRF
                     RVNVFYQRGYPAMVLRYISADMPKLDQLGLPDMFKDLIMMKRGLILMVGATGSGKSTS
                     IAAMINHRNETSSDHIVTIEDPIEFLHTNKRSIVNQREVGLDTKSFARALRGVMRAAP
                     DVILIGEIRDRETMEAAITLSGTGHLVLSTLHANNSAETMDRIINMFPQDQHKQIFLD
                     LSQYLRAVCSQRLVVTKEGKRIAAIEVMLNTPYISELVKKGDVSAIKEAIVASSERGV
                     QSFDVALLNLYKQNRIELEEALLNADSRSNLEAKINFG"
     gene            complement(1847..2377)
                     /locus_tag="VIV63_08915"
     CDS             complement(1847..2377)
                     /locus_tag="VIV63_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY6124759.1"
                     /translation="MLAAVAVLASCFLIYDALVFQSRSMQIRQLLVEAHPLERAPPET
                     LRRLFLIEAGTRLDIRVARSLMLKLEVSPISGGNMGDRLTTALWTSLVRLHLSEDEKI
                     ALIFSLSDLGNRVHGFQAAARSDFGRDLDRLDPREQALLVVYSQWPSGYRQPQHEELV
                     LVHRDSLINALANGTQ"
     gene            complement(2403..3182)
                     /locus_tag="VIV63_08920"
     CDS             complement(2403..3182)
                     /locus_tag="VIV63_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124760.1"
                     /translation="MSPDSIKGQATPESAMFDWKYWLGEFEGRVPRLMIARVPRDSLL
                     TSAERLRIADSIATFQLGEQSEGHTLLRFAREFGARNQCPALGRITELFIREEQHHAA
                     QLREFMLAHGIALKRRNWTDSVFRHIRRLAGFETAVTILVTAEMVGFVYYRALGRATE
                     NDYLRTICRQMCADEAIHLRYEAQLLRTLRGSRPRWKRAVVDWAHRSLLGIAARVVFR
                     EHRKVLGFAGYDARSFGKDCRSLYRTVLAQNPGSLRRRLVE"
     gene            complement(3381..>3886)
                     /locus_tag="VIV63_08925"
     CDS             complement(3381..>3886)
                     /locus_tag="VIV63_08925"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124761.1"
                     /translation="MVFLAEWMSAQNGPWKPLVLMGSEIPFPFKPRPSTILVPGIPAG
                     SIAAMPLLDGWSVPSRLASKAGFAGCYDGFVTQLADEWLGSLDAAAKAEVEVFSCGPT
                     PMLEAMAKVAAKHGVPCQVSLEEFMACAVGGCAGCTVKVMTPDGPAMKRVCVDGPVFD
                     ASAVFGH"
BASE COUNT          803 a         1217 c         1202 g          664 t
ORIGIN      
        1 gaatacgtgc accagcacaa gggctgcgtg atcacgcatc gcgaagtggg cgtggacacc
       61 gacaactggc acaccgcgct caagaacacg ctgcgccagg cgcccgacgt gatcctgatc
      121 ggcgaaatcc gcgatcgcga gaccatggag tacggcatcc agttcgccga aaccggccac
      181 ctggtgctgg cgacgctgca cgccaacagc tcgaaccagg cgctcgaccg catcatcaac
      241 ttcttccccg acgagcggcg cgaacagctg ctcatggatc tctcgctcaa catccgcgcg
      301 ctggtctcgc agcgcctcgt gccgcgcgaa gcgggcgcgg gccgcatcgc ggcgatggag
      361 atcatgctga actcgccgct catccaggac ctgatcttca aaggcaaggt gcaggagatc
      421 aaggaagtca tgtcgcgctc gaaccgcgtc ggcatgaaga ccttcgacca ggcgctctac
      481 gacctgtacg aacagggatt catttcctac gaagatgcgc tgcgcaacgc cgattcgaag
      541 aacgagctgc gactacgcat caagctcgag agcaagcgcg aagtgaagaa cctcgacgac
      601 ggcggcactc tgtcgatggt cgacgaagaa caacagggcc ggtcgttcta aaccggcgtg
      661 atttccagat agggcggata tgccagtcgt gaacgaaaag ttcgtggata ccattgaggt
      721 caagcaggcg tctgaaggcg cgccgatggt gctcaacacc cggccgctct tcaaactcat
      781 ggtcgagcgc aaggcgtccg acctgttttt cacctcgaat tcgcccatca aggtgaagat
      841 cgaaggcgcc atcctgccca tcaacaagca ggtgctgacg ccggagaccg tgcgccaggc
      901 ggcgtatggc ctgatgactc ccgatcagct ggaaagcttc aagcgcgagc tggaaatcga
      961 cttcgccatc tccgagcccg gcctcgggcg cttccgcgtc aacgtgttct accagcgcgg
     1021 ttatcccgcg atggtgctgc gctacatcag cgccgacatg ccgaagctcg accagctcgg
     1081 cctgcccgac atgttcaagg acctcatcat gatgaagcgc gggctcatcc tgatggtggg
     1141 cgccaccggt tcgggcaaat cgacttccat cgcggcgatg atcaaccatc gcaacgagac
     1201 ctcgtcggat cacatcgtga ccatcgagga ccctatcgag ttcctgcaca ccaacaagcg
     1261 ctcgatcgtc aatcagcgcg aagtgggcct cgacaccaag tctttcgcgc gcgccttgcg
     1321 cggcgtcatg cgcgccgctc ccgacgtcat cctcatcgga gaaatccgtg acagggagac
     1381 gatggaggcc gcgatcacgc tttccggtac gggccacctc gtgttgtcga cgctgcatgc
     1441 gaacaacagc gccgagacga tggatcgcat catcaacatg ttcccgcagg atcagcacaa
     1501 gcagatcttc ctcgatctgt cgcagtacct gcgcgccgtc tgctcgcagc gcctggtcgt
     1561 gaccaaggaa ggcaaacgca tcgccgccat cgaggtcatg ctgaacacgc cctacatcag
     1621 cgaactggtg aagaagggcg acgtctcggc gatcaaggaa gccatcgtcg ccagctccga
     1681 acgcggtgtg cagagcttcg acgtggcgct gctgaatctc tacaagcaga accgaatcga
     1741 gctcgaagaa gcgctgctga acgccgactc gcgttcgaac ctggaagcga agatcaactt
     1801 cggttgatgg gttgcgccgg cacgggtgcc ggtgcgaatt cgttcctcat tgcgttccat
     1861 tggccaacgc gttgatcaac gagtcccgat gcacgaggac gagttcttcg tgttgtggct
     1921 gccggtagcc gctgggccat tgcgaataga ccaccagtag agcctgttcg cgaggatcga
     1981 gtcgatcgag atcccgcccg aaatccgacc gggcggcagc ctgaaagccg tggacacggt
     2041 tgccaaggtc agaaagcgag aagatcaggg cgatcttttc gtcttccgac agatggagtc
     2101 gcaccagcga agtccataaa gcggtcgtaa gccgatcacc catatttccg ccagaaatcg
     2161 gcgaaacctc gagtttcagc atcaggctgc gcgcgacgcg gatatcaagg cgcgttcctg
     2221 cttcgatcag aaacagacgc cgcagggttt caggcggagc gcgctcgagc ggatgcgcct
     2281 cgactaacaa ctgtcttatc tgcatcgacc gggactggaa aaccaacgcg tcgtagatca
     2341 ggaaacaaga ggccagcacg gcgactgcgg cgagcacgcg cttgagcgcg aatcgagcca
     2401 tctcattcga ctagacgccg acgcagcgag cccgggttct gcgccagcac ggttcgatac
     2461 aacgaacggc agtccttgcc gaaactccgt gcgtcgtagc cggcgaaccc gagcactttg
     2521 cgatgctcgc gaaacaccac tcgagccgcg atgccaagca gtgagcggtg agcccaatcc
     2581 acgactgcgc gcttccatcg cggccgcgag cctcgcaatg tgcgcagcag ctgcgcctcg
     2641 tatcgcaggt gaatggcctc gtccgcgcac atctgccggc agatggttcg caggtagtcg
     2701 ttctccgtcg cgcgtccgag tgcgcggtaa tagacgaacc ccaccatttc cgcggtgacc
     2761 aggatggtca ccgcagtctc gaatccggcg agccggcgaa tgtgccggaa gacgctgtcg
     2821 gtccagttgc gccgcttgag cgcgatgccg tgcgccagca tgaattcccg aagctgcgcg
     2881 gcatggtgct gctcctcccg gatgaagagt tcggtgattc ggccgagcgc cgggcattgg
     2941 tttcgcgcgc cgaactcgcg cgcgaagcgc aacagcgtgt ggccttcgga ttgttcaccg
     3001 agctggaagg tggcgatgga atcggcgatg cggagccgct cggcggaagt cagcaggctg
     3061 tcgcggggca cgcgcgcgat catcagccgc ggaacgcgcc cttcgaattc gcctagccag
     3121 tacttccagt cgaacatcgc ggactccggc gtcgcctgac ccttgatcga gtctggcgac
     3181 accgcgagcg gcgatggtga tatgtcagga cggaatcatg gcgaattatg gcggcgtcaa
     3241 ttgcacgccg tggcgctcag ccagcgggac ctgcgtggcg ccaagtacca cgaagattcc
     3301 gggtgcgaga gtcagtgttg tcaggtcggt gtcgtcggct tccatttccg tggaacgcca
     3361 aacgcgcgcg gtctgggttc tcagtgcccg aagacagcgc tcgcgtcgaa cacagggcca
     3421 tcgacgcaga cgcgcttcat cgcgggcccg tcgggcgtca tcaccttcac cgtgcagccg
     3481 gcgcagccgc ccacggcgca ggccatgaac tcttcgagcg agacctggca tggcacgcca
     3541 tgtttggctg ccactttggc catggcttcg agcatggggg tggggccgca ggagaacact
     3601 tcgacttcgg ctttcgcggc cgcgtcgagc gagcctaacc actcgtccgc gagctgcgtg
     3661 acgaagccgt cgtagcaacc ggcgaagccg gccttgctgg cgaggcggct cggcacgctc
     3721 cagccatcga gcagtggcat ggcggcgatg ctgccggccg gaatgcccgg caccagaatg
     3781 gtcgacggcc gcggcttgaa ggggaaggga atctcggagc ccatgagcac gagaggtttc
     3841 cagggaccat tttgcgcaga catccactcg gcgaggaaca ccatgg
//
LOCUS       DATDEU010000376         5463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_507434, whole genome shotgun sequence.
ACCESSION   DATDEU010000376 DATDEU010000000
VERSION     DATDEU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507434"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VIV63_08930"
     CDS             complement(<1..459)
                     /locus_tag="VIV63_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124762.1"
                     /translation="MKTRTTYGVLIWAATLLLAGCGSGGDEDQTADELSGEFQAGGVS
                     GLRYVTPTRSGVTDGAGTFKYLAGETVTFSVGGITLGHAPGSSGITPFTLAGMTPPTT
                     EAALRRELDRASRTTSHFVRATNMMRLLLALVADSNPANGIDLRGRDAALA"
     gene            563..808
                     /locus_tag="VIV63_08935"
     CDS             563..808
                     /locus_tag="VIV63_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY6124763.1"
                     /translation="MDDKSKNLEAVLSKMVHDLRTPLAVVHTTTNMLLNPKYQFSETQ
                     VREQHERIQRNVEVMNRLLGELSEFARTANPDAGDGA"
     gene            798..2972
                     /locus_tag="VIV63_08940"
     CDS             798..2972
                     /locus_tag="VIV63_08940"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY6124764.1"
                     /translation="MARSAVLPAFSLAFLAALSGVTPGVAATRPPAPVYQAQVINAEL
                     TGGLLVGGSQVLLVWGSDGTILRSEDGARWLHAVTPTSADLIRATSNDGGGVIVAVGA
                     QGTILRSTDAGRTWQAARTTTLDTDLRAVVNHPGTRTWVAAGTNGRLVRSIDDGKNWS
                     LVDSQLKVDFQTLFVDTKTQFILIGGDEGMVGHSKDAGMSWQVTALAMPDPATPITAF
                     HRFGKLLLATSALGRFLTSEDDAQSWDLMQAGTNAFFTDCAFDPVRDAIVMTGHNGDV
                     LRSPDGGKSWEGGEVTLDGRKNFLTSIRFDERSGSLIVIGQGGTVARSIDGGVSWSRA
                     SEDFHGDVRGLVKDTVRNRLVAFGAGGMVMSSIDSGIRWNDDRTSLDLWLREIEATPR
                     GNALIATSKLGEIIRSTDGGTNWRVMPVTYPNPNTPPDLRGLAVTPSGNAMIAVGPPG
                     AILRSDPDGNAWVVRHSTPIEAERALPWVLVDAQQKILVAVETRGEMQVSRDDGETWQ
                     ASSIPIAEGKWPFWHGTVHAGVMLIGGQSGKAARSTDGARTWSYVDTGTDKDVFGSFA
                     NEVTGTFFLAGADGTLLRSTDLGVSWKNIPTGSTQELRRLMRDPRTGALLCYGAHGTI
                     LRSQDDGLTWRPASSGTDGVLRKGVLEPRTGNLLLVGGQGALLRSRDGGRTWEVLPTH
                     TPRHFSSMWADDRTGDLVLVGERIVRLVRPLNGKIGRANEQP"
     gene            2974..4110
                     /locus_tag="VIV63_08945"
     CDS             2974..4110
                     /locus_tag="VIV63_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124765.1"
                     /translation="MPSAIFNRRFAANYFSTLGFIGLAYWVVSDLSGFHRSILQGQWQ
                     FGMFEIDAVLTIHTLFLLLIALYAVVLIPFYARYPWLHSKAFVFLRGLHFSLVRARRP
                     QSRARHALQARNPWRLPLKAGLSPAAKQAGLALLLKFFFAPLMINWCLNHAGDAANSV
                     VQFFDGLKEGLSGRELFDSSLFWTCFQLILFVDTLLFTLGYIIEVPALGNRIRSVDPT
                     FFGWFICLACYPPFNDFTGRFLEWQSNDFPHFANSAVHFIANITLLTALVIYSWASVA
                     LGFKASNLTNRGIVGHGPYAYVRHPAYAAKNLAWWIGALPTLYMAFASGNVRIAGYAV
                     LALCGWTTIYALRAITEERHLLLANNGYAQYMTRVKWRFIPGIV"
     gene            complement(4121..4960)
                     /locus_tag="VIV63_08950"
     CDS             complement(4121..4960)
                     /locus_tag="VIV63_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124766.1"
                     /translation="MSVRSVIVPALLVFVAVVWWASREGGEPPLADAGSANPQASRAV
                     HDHWTTVAGTRAEAPADDGPHVRSDDVIAEEGSLGTYTDGKYRFLFAESGQSPEAARK
                     LRAALLARERIAVAINTARQSSDPAAKEAIPVQLAELAALDQKIGTLLRPGDLATFEV
                     LKDSDIEQFQLDDYAGGVSAVAPLNDADRKAILYTKLVHRQRFRQVLAESGLMRGDLS
                     ASERQLAFADVSRSLKASRDSYLQEVRQYLYNDEQFTLLSNYENTEYAAELEKLRGIA
                     NGE"
     gene            complement(5076..>5463)
                     /locus_tag="VIV63_08955"
     CDS             complement(5076..>5463)
                     /locus_tag="VIV63_08955"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEY6124767.1"
                     /translation="NGGNLQNFRWTNIGEDAFTVKSSGTVNISGVSGFNGEDKFGQIN
                     AAGTVNLSNCIVDGVAKVIRQNGGTSFKTTVNIDRCQLSNLKEGVFRSDSSSSVARLT
                     NSRLRNSGSLCIGSWSSCTGSGNTNY"
BASE COUNT          968 a         1771 c         1685 g         1039 t
ORIGIN      
        1 ggccagcgcg gcatcccgcc cgcgaaggtc gatgccattg gcgggattgc tgtccgcaac
       61 gagcgccagc agcagtcgca tcatgttggt ggcgcgtacg aaatggctgg tagtgcgcga
      121 agcacggtcg agttcccgcc gcagcgccgc ttccgtggtg ggcggagtca tgccggccag
      181 cgtgaatgga gtgatgccgg acgagcccgg cgcatgcccg agcgtgatgc cacccacgga
      241 gaatgtgacc gtttctccgg ccagatattt gaacgtccct gccccgtccg tgacgcccga
      301 tcgcgtcggc gttacatagc gcagaccgct gacgcctccc gcctgaaact ctccgctaag
      361 ttcgtcggct gtctgatctt catcgccgcc actgccacag cctgccaaaa gaagcgtcgc
      421 ggcccagatc aggacgccgt aagtagttcg agttttcatc atatgaatgc ttctgccacc
      481 gggagcaact tcctgacgtc aaggctggat atgctccccg gcgaaggtta ccgtctggac
      541 tccacgagga ttggagcggg aaatggacga taaatcaaag aacctggaag ccgttctgtc
      601 aaagatggtc cacgaccttc gcacgccgct ggccgtggtg cacaccacca ccaacatgtt
      661 gctcaatccc aagtaccagt tcagcgaaac gcaggtccgc gagcaacacg agcgcatcca
      721 gcgcaacgtc gaagtgatga accggctgct cggggaactc tcggaattcg ccaggaccgc
      781 gaaccccgat gcgggcgatg gcgcgtagcg ctgtattgcc ggcgttctcg ctggcattcc
      841 tggcggcgct ttccggggtc acaccgggtg tagccgcaac gcgcccgccc gctccggtct
      901 atcaggcgca ggtcatcaac gccgaactga ccggcggttt gctcgtcggc ggctctcaag
      961 tgctccttgt ctggggcagc gacggaacga tcctgcgctc cgaagacggc gcgcgctggt
     1021 tacacgcagt gacgccgacg tcggcggatc tgattcgcgc cacctcgaac gacggcggcg
     1081 gcgtaatcgt ggcggtcgga gcgcagggca ccatcctgcg gtcgacggat gctggccgca
     1141 cgtggcaggc agcgcgcacc acgaccctcg acaccgacct gcgtgcagtg gtcaaccatc
     1201 cgggaacccg aacgtgggtg gcggcgggta cgaatggacg cctggtgcgc tcgatcgacg
     1261 atggcaagaa ctggagcctg gtcgattcgc agctcaaggt ggatttccag accctgttcg
     1321 tcgacacaaa aacacaattc atcctgatcg gcggcgacga aggcatggtc ggccactcga
     1381 aggacgccgg aatgtcatgg caggtcaccg cgctcgcgat gcctgatccg gccacgccca
     1441 tcaccgcctt ccaccgcttc ggcaaactac tgctcgcgac cagcgcgctc ggccgattcc
     1501 tgacgtccga agacgatgcg cagagctggg acctcatgca ggccggcacc aatgccttct
     1561 tcaccgactg cgcgttcgat ccggtgcgtg acgcaatcgt catgactggc cacaacggcg
     1621 acgtgttgcg ttcgccggac ggcggcaaga gctgggaagg cggagaagtc acactcgatg
     1681 gccgcaagaa ctttctcacg tcgatccgct tcgatgagcg cagcggctcg ctgatcgtca
     1741 ttggccaggg cggcacggtg gcgcgctcca tcgacggcgg agtgtcgtgg agccgcgcgt
     1801 cggaagactt ccacggcgac gtgcgcggcc tcgtcaaaga caccgtgcgg aaccgcctgg
     1861 tggccttcgg cgcgggcggc atggtgatga gctccatcga ttcgggtatt cgctggaacg
     1921 atgaccgcac ctctctcgat ctatggctgc gggaaatcga ggcgacgccg cgaggcaatg
     1981 cgctcatcgc caccagcaaa ctcggcgaga tcatccgctc gacggatggc ggtacgaact
     2041 ggcgagtgat gccggtcact tacccgaacc ccaacactcc gcccgacttg cgcggattgg
     2101 ccgtgacacc gtcgggcaat gccatgatcg cggttgggcc gccgggcgcc attctccgat
     2161 cggatcccga tggaaacgca tgggtcgtgc gtcactccac gcctatcgaa gcggaacgcg
     2221 cgcttccgtg ggtgctcgtc gacgcgcagc agaaaattct cgtcgcggtc gagacgcgcg
     2281 gcgaaatgca ggtgtctcgc gatgacggtg aaacctggca ggcgagcagc attcccatcg
     2341 cagaagggaa gtggccgttc tggcacggca ccgttcacgc cggtgtcatg ctcattggcg
     2401 gccagtctgg caaagccgcg cgcagtactg acggcgcgcg aacgtggagc tatgtcgata
     2461 cgggcacgga caaggacgtc ttcggcagct tcgccaatga agtcaccggc acattctttc
     2521 tcgcgggcgc cgacggcacc ttgttgcgct cgaccgatct cggcgtttcg tggaagaaca
     2581 tcccgaccgg gagcacccag gaacttcggc gcctgatgcg cgatccgcgt accggcgcgc
     2641 tgctgtgtta cggcgcgcac ggcacgatcc tgcgttcgca ggatgacggc ctgacgtggc
     2701 gcccggcttc gagcggcaca gacggtgtgc tgcgcaaagg cgtgttggag ccgcgcacgg
     2761 gcaatctact gctcgtgggt ggacagggcg cactgctccg ctcacgcgac ggcggccgca
     2821 cctgggaagt gctcccgact cacaccccgc ggcacttcag cagcatgtgg gccgatgatc
     2881 gtaccggcga tctcgttctg gtcggcgaac gaatcgtgcg gctcgtgcgc ccgttaaatg
     2941 gcaagattgg ccgcgctaac gaacaaccat gacatgccct ccgcgatctt caaccggcgt
     3001 ttcgccgcca actatttctc gacgctcggc ttcataggcc tcgcgtattg ggtcgtttcc
     3061 gacctgagcg gctttcaccg cagcatcctt caaggccagt ggcagttcgg catgttcgag
     3121 atcgatgccg tgctcaccat ccacacgctg ttcctgctcc tgatcgcgct gtacgcggtg
     3181 gtgctcattc ctttctatgc gcgttatccc tggctgcatt ccaaagcgtt cgtattcctg
     3241 cgcgggttgc acttcagcct ggttcgcgcg cgccgcccgc aatcgcgcgc acgacacgca
     3301 ctgcaggccc gaaacccatg gcggcttccg ctgaaagcgg ggctctcgcc ggctgccaag
     3361 caggcgggcc ttgcgctgct gctcaaattc ttcttcgcgc cgctgatgat caactggtgt
     3421 ctgaaccatg ccggcgatgc ggcgaattcc gtggtgcagt tcttcgacgg cctgaaggaa
     3481 ggactgtcgg gacgcgaatt gttcgacagc tcccttttct ggacctgctt ccagctcatc
     3541 ctcttcgtcg acacgctgct gttcacgctc ggctacatca tcgaagtgcc ggcgctcggc
     3601 aatcgcatcc gctcggtgga tccgaccttc ttcggctggt tcatctgcct cgcgtgttat
     3661 ccgccgttca atgacttcac cggccggttc ctcgaatggc agagcaacga tttcccgcac
     3721 ttcgccaact ccgcggtgca cttcatcgcc aacatcacgt tgctgaccgc gctcgtaatc
     3781 tattcgtggg cctccgtggc gctcggattc aaggcgagca acctcaccaa ccgcggcatc
     3841 gtcggacatg gcccgtacgc gtacgtgagg catcccgcgt acgccgccaa gaacctcgcc
     3901 tggtggatag gcgcgctgcc gacgctctac atggcgttcg ccagtggcaa cgtgcgcatc
     3961 gccgggtacg cggtgctcgc cctgtgtggc tggaccacca tctacgcgct gcgtgcgatc
     4021 accgaagagc gacatctgtt gctcgcgaac aatggctatg cgcaatacat gacgcgcgtg
     4081 aagtggcgct tcatccccgg catcgtctga agcctcgggc tcattcgccg ttggcgatgc
     4141 cgcgaagctt ctcgagctcg gcggcatatt cggtgttctc gtaattgctg agcagcgtga
     4201 actgctcgtc gttgtacaga tactggcgaa cttcctgcag atagctgtca cgcgaagcct
     4261 tcagactacg gctcacgtcg gcgaacgcca gctggcgctc actggcgctc aggtcgccac
     4321 gcatgagacc ggattcggcc agcacctgcc ggaaacgctg ccgatgcacc agcttggtat
     4381 agaggatcgc cttgcggtcc gcgtcgttga gtggcgcgac ggcgctgacg ccgcccgcgt
     4441 aatcgtcgag ctggaactgc tcgatgtccg aatccttgag gacttcgaac gtcgccagat
     4501 cccctggccg gagcagcgtg cctatcttct ggtccagcgc ggcgagttcg gcgagctgga
     4561 ctggaattgc ctccttcgca gcgggatcac tcgattgcct ggcggtattg atcgccacgg
     4621 cgatccgttc gcgggccagc agcgccgcac gaagcttgcg cgccgcctcc ggtgactgtc
     4681 ccgactcggc gaacaggaac cgatatttcc catcggtgta ggtgccaagg ctgccttcct
     4741 cggcgatcac atcatcggac cgaacgtgcg gaccgtcgtc ggccggtgct tcagcgcgag
     4801 tgccggcaac ggtggtccag tggtcgtgca ccgctcgcga ggcttgcgga ttcgcggacc
     4861 ccgcgtcggc cagcggcggc tcgccgccct cgcggcttgc ccaccaaacg acggccacga
     4921 acacgagtag cgccggcacg attacgcttc gtacgctcat cgagtccagc gacctcgctt
     4981 ccgcggccat gtttcctccg ggacgaaatg ccgggggtgt taccaccccc ggccaagcta
     5041 ccgttttcat ccttaaaact ggcagcttgt tccgatcagt agttcgtatt ccccgaaccc
     5101 gtgcagctcg accatgagcc gatgcacagc gaacccgagt tacgcagacg gctgttggtc
     5161 agccgagcca cgctgctcga gctgtcggag cggaacacgc cttccttcag gttcgacagt
     5221 tggcagcggt cgatgttgac cgtggtcttg aaggaggtgc cgccattctg acggatgacc
     5281 ttcgcgacgc cgtccacgat gcaattggaa aggttgaccg ttccagcggc attgatctgg
     5341 ccaaacttgt cttcgccatt gaagcccgag acaccggaga tgttcacggt gcccgaggac
     5401 ttcacggtaa aggcgtcttc accgatgttg gtccagcgga agttctgcag gttaccgccg
     5461 ttg
//
LOCUS       DATDEU010000377         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1819142, whole genome shotgun sequence.
ACCESSION   DATDEU010000377 DATDEU010000000
VERSION     DATDEU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819142"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VIV63_08960"
     CDS             <1..555
                     /locus_tag="VIV63_08960"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HEY6124768.1"
                     /translation="RDETRTSKAMLEDTIGAPVLGYRAASYSITRESLWAIDILCDLG
                     FRYDSSIFPIAHDRYGIPGAATRPGVMKDSRGNGIVEFPLSTVKFMGRRLPCSGGGYF
                     RLLPYAYTRWALKKINKDDQLPFIFYLHPWEIDPGQPRFNASRLSRFRHYTNLSVCEK
                     RLRRLVDDFRFTTVREVLRPQLDA"
     gene            575..2500
                     /gene="asnB"
                     /locus_tag="VIV63_08965"
     CDS             575..2500
                     /gene="asnB"
                     /locus_tag="VIV63_08965"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY6124769.1"
                     /translation="MCGLAGIHLDSREARPDRERVERMIASIGHRGPDGTGFHIDPGV
                     ALGHARLSIIDLGGGAQPIHNGDETVWISYNGEVFNYVELRQWLEERGHKFYTHTDTE
                     VIVHLYDELGDEFVHELNGQFAFALWDRKRRRLLLVRDRAGILPLYYARVAGGVAFGS
                     EVKTLIASGLVQPAMDAVGLDELMTFWAPVAPRTVFKGVSQVCPGEMVILEGASLTRK
                     QYWDWDFPGASQHRRESQGVLEEELRSILTSATEIRLRADVPVGAYLSGGLDSSTLVA
                     LLKERLPDTLETFSIGFDDVGLDESAHQRTVADYLKTQHHHVQCSRADIASVFPATIR
                     HGEMPVLRTAPAPMQLLSGLVRRSNVKVVLTGEGADEVLGGYDIFKESKVRQFWAEQP
                     ESSWRPALLKRLYPYLDLTSAQSSAYLKEFFGIGLSNPADPVFSHLPRWATTAQVKLF
                     WSDDLRSQVQGSAIERLRESVSAKLKPMAPFNRSEYLEAKTLLPGYLLASQGDRMLMA
                     NSVEGRFPFLDHRLIAFANQLRPNVKMRVLREKHLLKEMMRSRLPESILKRFKQPYRA
                     PDAAAFFEGNAPGYVADLLSESRVRDYGYFDASKVTRLAAKLRKAQVPSARDNMAFVG
                     VLSTQLWHAQFVAGEKP"
BASE COUNT          452 a          848 c          811 g          473 t
ORIGIN      
        1 cgcgacgaga cgcgcacctc gaaggcgatg ctcgaggaca ccatcggcgc gccggtgttg
       61 ggttaccgcg cggccagcta ttccattacg cgcgagtcgc tgtgggccat cgacatcctc
      121 tgcgatctcg gtttccgcta cgactccagc atcttcccga tcgcgcacga tcgctacggc
      181 attccgggtg cggccacgcg gcccggcgtc atgaaggaca gccgcggcaa cggcatcgtg
      241 gaattcccgc tatctaccgt gaagttcatg gggcggcgcc tgccgtgctc gggcggtgga
      301 tacttccggc tgctgcccta tgcgtacacg cgctgggcgc tgaagaagat caacaaggac
      361 gaccagcttc cattcatctt ctacctgcat ccctgggaga tcgatcccgg gcagccgcgt
      421 ttcaacgcga gcaggctgtc gcgcttccgt cactacacca acctgtcggt gtgcgagaag
      481 cgcctgcggc ggctggtgga cgatttccga ttcaccacgg ttcgcgaagt gctgcgtccg
      541 cagctggacg cgtgaggaac ggcgacgaac tcttatgtgt ggacttgctg gcatacacct
      601 ggactcgcga gaggcgaggc cggatcggga acgcgttgaa cgcatgatcg cgtccatcgg
      661 gcatcgtggg cccgatggca ccggattcca tatcgacccg ggcgttgcgc tgggccatgc
      721 gcggctttcc atcatcgacc tgggcggcgg tgcccagccc atccacaacg gcgatgagac
      781 cgtgtggatc agctacaacg gcgaagtgtt caactacgtc gagctgcgcc agtggctcga
      841 ggaacgcggc cacaagttct atacgcacac cgacaccgaa gtgatcgtgc acctgtacga
      901 cgaactcggc gacgagttcg tgcacgagct caacggccag ttcgcgttcg cgctctggga
      961 tcgcaagcgc cgccggctgc tcctggtgcg cgatcgcgcc ggcatcctgc cgctgtacta
     1021 cgcgcgcgtc gccggtggcg tggcgttcgg ttcggaagtg aagacgctga tcgcgagcgg
     1081 cctcgtgcag cccgcgatgg atgccgtcgg cctcgacgag ctcatgacct tctgggcgcc
     1141 ggtcgcgccg cgcacggtat tcaaaggcgt ctcgcaggtg tgtcctggcg agatggtgat
     1201 cctcgaaggc gcaagcctca cgcgcaagca gtattgggat tgggattttc ccggcgcatc
     1261 gcagcaccgc cgcgagtcgc agggcgtgct cgaggaagag ctgcgttcca tcctcacgag
     1321 cgccacggaa atccggctac gcgccgacgt gcccgtgggc gcctatctgt cgggtggcct
     1381 cgactcctcg acgctggtcg cgctgctcaa ggagcgcctg ccggacacgc tcgaaacttt
     1441 ctcgatcggc ttcgacgacg tgggattgga tgaatccgcc caccagcgca cggtcgccga
     1501 ctacctgaag acgcaacacc accacgtgca atgttcgcgc gcggatatcg ccagcgtgtt
     1561 cccggccacg atccgtcatg gcgagatgcc ggtgctgcgc accgcacccg cgcccatgca
     1621 gttgttgtcc ggcctcgtgc gccgatccaa cgtgaaggtg gtgttgacgg gcgagggcgc
     1681 cgacgaggtc ctcggcggtt acgacatctt caaggaatcg aaggtgcgcc agttctgggc
     1741 cgagcagccc gagtcgtcct ggcgtccggc gctgctcaag cggctgtatc cctatctcga
     1801 tctcacgagc gcccagagct ccgcatacct caaggaattc ttcggcatcg gcctgtccaa
     1861 tcccgccgac ccggtgtttt cgcatctgcc tcgctgggcg accaccgcac aggtcaaact
     1921 attctggtcc gacgacctgc gctcgcaggt gcagggatcg gccatcgaac gcctgcgcga
     1981 atcggtcagc gccaagctca agcccatggc gccgttcaac cgcagcgagt acctggaagc
     2041 gaagacgctg ctgccgggtt acctgctggc ctcgcagggc gatcgcatgc tcatggcgaa
     2101 ctcggtcgaa ggccggttcc cgttcctcga ccaccgcctg atcgcgttcg ccaaccagtt
     2161 gcggccgaac gtgaagatgc gcgtgttgcg cgagaaacac ctgctcaagg aaatgatgcg
     2221 ttcgcggctg ccggagtcca ttctcaagcg gttcaagcag ccgtaccgcg cgcccgatgc
     2281 cgcggcgttc ttcgagggta atgccccggg gtacgtcgcc gacctgctca gcgaatcgcg
     2341 cgtgcgcgat tacggctact tcgacgcttc gaaggtgacc cgcctcgcag ccaagctgcg
     2401 caaggcgcag gtgccatcgg cgcgcgacaa catggctttc gtcggcgtgt tgtcgacgca
     2461 gctctggcac gcgcagttcg tcgccggcga gaagccttga gaatttttca tcactatcaa
     2521 atgaggaacc cacaatgtcc gacgccctga ccgaccagat ccgcgatttc atcctcgaga
     2581 acta
//
LOCUS       DATDEU010000378         6125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_359741, whole genome shotgun sequence.
ACCESSION   DATDEU010000378 DATDEU010000000
VERSION     DATDEU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359741"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VIV63_08970"
     CDS             complement(<1..343)
                     /locus_tag="VIV63_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124770.1"
                     /translation="MSTAELLARLARLDARDRAWLLGELPPGLRRELADVLADDEPVA
                     SAAPVAPAPLAGWESIDPSRAAEVLGAEPAWLASAATRGADVQWRERLLQAMPSAKRR
                     EIEIADRAARTL"
     gene            complement(340..1755)
                     /gene="fliF"
                     /locus_tag="VIV63_08975"
     CDS             complement(340..1755)
                     /gene="fliF"
                     /locus_tag="VIV63_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY6124771.1"
                     /translation="MKLETMSARTRVTLGIGIVLILAATVVFAWWAMRTEYSVLFSRL
                     TEADAAGIVAELKKQKTEYQLADGGTTIRVPADRVHDVRLALMSSGTPIAGGVGFEIF
                     DRQGLGATEQSQRVSFQRALQGELARTIGALEGVNQARVHLVLPESTLFRRDRQDARA
                     SVSLKLEPGVVLQNPQIFGIQRLVAAAVPGLDIGRVVVTDQHGLTLSAADALTVGGAT
                     GEGRLSMKREVEEYVTRKIVALLDRAYGPGRAIVSVDATLNFDEVRRTVQDLLPPNGQ
                     NVRRRRQTTGGAMAVDASAFDDAGAGARGSIDTEYDYGRRVEQVVAAPGGVTRLSVGV
                     IVPGATDLATLQRIEDLVRVAAGINDSRDDLVVVQSLDGLGPDTVGAVAPVTAVLEPV
                     EQAAAAPAVAIVATPWWNHWYVWAGAGLLFAALFLLALLGRGGGSGERLSADERAKLL
                     ADVRASLERGGPRMTAAGGAS"
     gene            complement(1752..2123)
                     /locus_tag="VIV63_08980"
     CDS             complement(1752..2123)
                     /locus_tag="VIV63_08980"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY6124772.1"
                     /translation="MSIEAISAVSAQLATVAPAATPALITAPPADPAALASMSDLLAT
                     QPGVSPGEGLFDQLVSSLGEIDAGAARNTEAATELALGQTDNLHQVLIGAERTRLQFE
                     LAMSMRNRVLEAYQEIMRMQV"
     gene            complement(2120..2557)
                     /gene="flgC"
                     /locus_tag="VIV63_08985"
     CDS             complement(2120..2557)
                     /gene="flgC"
                     /locus_tag="VIV63_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY6124773.1"
                     /translation="MGMFGVLDISSAGMDVQQARLEVAASNIANSRTTGEGGAAYKPL
                     TVVVRSAIAEAQGAAGTPVSTAQLPMPMVAEVVEQDVAPKLVYDPGHPNADARGMVSL
                     PGVDPITSMLDLIEISRGYEANLRAFDITRSLLQRTLDLGRGR"
     gene            complement(2562..2924)
                     /locus_tag="VIV63_08990"
     CDS             complement(2562..2924)
                     /locus_tag="VIV63_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124774.1"
                     /translation="MLNADPVLALLSRALDAAALRQTVHATNVANADTEDFRRLEVIY
                     SAELQRLQANGPALDVQQALEWAQAEPEVVESTETTVRLDQEMAQMAENAVRYQALLG
                     AIDRTLGMLRTAARDGKE"
     gene            complement(2936..4045)
                     /locus_tag="VIV63_08995"
     CDS             complement(2936..4045)
                     /locus_tag="VIV63_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY6124775.1"
                     /translation="MSKTLLYIALALIVAADAQSAVRIKDLARFDGVREQPIIGYGLV
                     VGLSGTGDSARSFTTLQSVSNMLREFGLAVPPYAVSSRNVAAVIVTADLPGAVRAGDR
                     FDVNVAAIGDARSLAGGTLLMTPLMANNKVSYAMAQGPLAVGGYKFEQRGTSAQKNFP
                     TAGTIPEGAIAERTLEPEILQSNGALELLLDEPDYTTATRVVAAINAALPGGNATAID
                     SGRVRVSLNGDAGTAVAMMARIESLAVDPDVAARVVVNERTGTVVSGGDVYLSAVTVT
                     HGDLRVSIVERYNVSQPEGIYYGRQSGIRTVVAPEATVSVSEDEAQAVSLDSGATIAD
                     LVAALRAIKVSSRDVIAILQGIKRAGALHAELVIQ"
     gene            complement(4049..4624)
                     /locus_tag="VIV63_09000"
     CDS             complement(4049..4624)
                     /locus_tag="VIV63_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY6124776.1"
                     /translation="MKTQHSAVLLALALAPLAPGAFAADATSAPRSLFADRRAHMPGD
                     LITVLIAEAASVSASAQTRTSKGESASANLLQRDGEMQLANAGFEGRFAGGGEIERKG
                     ALLARLSVRIDAVDSNGNFIVSGEQLIVINNEKQTIRVSGLVRPDDIAADNTVPSWRV
                     AGTDIQLLGKGLLSKKSSPGLLTRLLSLFGQ"
     gene            complement(4621..5283)
                     /gene="flgA"
                     /locus_tag="VIV63_09005"
     CDS             complement(4621..5283)
                     /gene="flgA"
                     /locus_tag="VIV63_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY6124777.1"
                     /translation="MEHALLIRAFALASIVLVCVARSAHAGEAAPALEPHLREQRPDV
                     LRWQWRPLSESANAVPEAGIAGVGRVGSRTAVRFTDGRVRWYAVVGMSQVLVSAHALD
                     RGAAFTADDAELAEHDVIALGCEPAKPLDAGQRWRATRRLVAGEALCAKDIEIVPDVE
                     RDRPVTLNAQRGAVSASRVLTAAADARFGERVRLRDRTNGITVSAIVTGPGAARLSEE
                     QE"
     gene            complement(5283..6062)
                     /gene="flgG"
                     /locus_tag="VIV63_09010"
     CDS             complement(5283..6062)
                     /gene="flgG"
                     /locus_tag="VIV63_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY6124778.1"
                     /translation="MTDALYVGATGMRAHQTQVDTIANNVANVNTVGFRRESVNFSSV
                     MAALQSGATDPLGSQLRETLQSRGAGAIASLQLSTQAGELKQTQAPLDVAIDGAGFFE
                     VLLPDGTPAYTRAGALSLNSEGMLAGAGLPLSARIAVPSDTRDLRITNDGRVMATVAD
                     RSDVELGNIELAAFANPAGLQSIGDNLYQATGASGEMQLGAPGEAGNGQLRQGFLESS
                     NVQLVDELVSLMLAQRGFEMNSKIVQAADQMLGITNSLYRG"
BASE COUNT          940 a         2119 c         2054 g         1012 t
ORIGIN      
        1 cgagcgttcg cgcggcgcga tccgcgatct cgatctcgcg gcgcttggcg gacggcatgg
       61 cctgcaacag ccgttcgcgc cattggacat cggcgccgcg cgtcgcggcg ctggcgagcc
      121 acgctggttc cgcgccgagc acttcggccg cgcgtgatgg gtcgatcgat tcccagccgg
      181 ccaatggcgc gggagccacc ggggccgctg acgccacggg ctcgtcgtcc gccaacacat
      241 cggccagctc gcgccgcaat ccgggcggga gctcgcctaa caaccaggcg cgatcccgcg
      301 cgtcgaggcg cgcgagccgg gccagcagtt ccgccgtgct cacgaggccc ctccggccgc
      361 ggtcatgcgc ggaccgccac gttcgagcga ggcgcgcaca tcggcgagca atttcgcgcg
      421 ctcgtcagcc gacagacgtt caccgctgcc gccgccgcgc cccaggagcg cgagcagaaa
      481 cagcgccgcg aacagcaggc ccgcgccagc ccacacgtac caatggttcc accagggcgt
      541 tgcgacgatc gcgaccgccg gcgccgcggc tgcctgctcg actggttcca gcacagccgt
      601 cacgggcgcc acggcgccga cagtatcggg tccgagcccg tccaggctct gcaccaccac
      661 caggtcgtca cgcgaatcgt tgattcccgc cgccacgcgc accaggtctt cgatgcgttg
      721 cagggtcgca agatcggtgg cgcccgggac gatgaccccc accgacagcc gcgttacgcc
      781 gccgggcgcc gctacgacct gctccacgcg gcggccgtaa tcgtattccg tatcgatgct
      841 tccgcgcgct cccgcacccg cgtcgtcgaa cgccgaggcg tcgaccgcca tggctccgcc
      901 ggtagtttga cggcggcgtc gcacgttctg gccattgggc ggcagcaggt cctgcaccgt
      961 gcgccgcact tcatcgaagt tgagcgttgc gtcgacgctc acgatggcgc ggcccggccc
     1021 gtaggcgcga tcgagcagcg cgacgatctt gcgcgtcacg tattcctcga cttcgcgttt
     1081 catggacagc cggccttcgc cggtcgctcc tccgacggtg agtgcatcgg ccgcggacag
     1141 cgtgagaccg tgctgatcgg tcacgaccac ccggccgatg tcgagcccgg gcacggccgc
     1201 ggccacgagg cgctgaatgc cgaagatctg cgggttctgc agaacgacac ccggctcgag
     1261 cttgagactc accgaggcgc gtgcgtcctg ccggtcgcgg cggaacaacg tggactccgg
     1321 caacaccagg tgcacgcgcg cctggttcac gccttcgagc gcgccgatgg tccgcgccag
     1381 ctcgccctgc aacgcgcgct ggaaagacac gcgctgtgat tgttcggtgg cgcccagtcc
     1441 ctgccggtcg aagatctcga acccgacgcc accggcgatc ggcgtgccgc tcgacatgag
     1501 cgccaggcgc acgtcgtgga cgcgatcggc cgggacgcgg atggtggtcc caccgtcggc
     1561 gagctggtac tccgttttct gcttcttgag ctcggcgacg atgcccgccg catccgcctc
     1621 ggtgagccgg ctgaacagca ccgaatattc ggtacgcatc gcccaccacg cgaacaccac
     1681 ggtcgcggcc agaatcagca cgattccgat tccgagcgtg acgcgcgtgc gcgccgacat
     1741 cgtttctagt ttcatacctg catccgcatg atttcctggt acgcctcgag cacacgatta
     1801 cgcatcgaca tcgccagctc gaactgcagc cgcgtgcgct cggcgccgat gagcacctgg
     1861 tgcaggttgt cggtctggcc aagtgcgagt tcggtggccg cttcggtgtt gcgcgcagcg
     1921 cccgcgtcga tttcgccgag cgagctcacg agctgatcga acaggccttc gccgggcgaa
     1981 acgccaggct gcgtggccag cagatccgac atggacgcga gcgccgccgg atcggcaggc
     2041 ggagcagtga tcagcgccgg cgtagcggcg ggcgcaacgg tggcgagctg cgcgctcacc
     2101 gcggagatgg cttcgatgct catcggcccc tcccgaggtc cagcgtgcgc tgcaggagcg
     2161 agcgcgtgat gtcgaacgcg cgcaagttgg cttcgtagcc gcgcgagatc tcgatgagat
     2221 ccagcatgga ggtgatcggg tccacacccg gcagcgacac catgccgcgc gcgtcggcat
     2281 tcggatggcc cgggtcgtag accagtttgg gcgcgacgtc ctgctccacg acctcggcga
     2341 ccatgggcat ggggagctgc gccgtgctga ccggtgtacc cgccgcacct tgcgcctccg
     2401 caatggcact gcgcacgacc acggtcagtg gtttgtaggc cgcgccgccc tcgcccgtcg
     2461 tgcgcgagtt ggcgatgttg ctggcggcga cttcgaggcg tgcctgctgc acgtccatgc
     2521 ccgccgagct gatatcgagg actccgaaca ttcccatgga cctactcctt gccgtcgcgc
     2581 gctgcggtgc gcagcattcc gagcgttctg tcgatggcac ctaacaacgc ctggtaacgc
     2641 acggcgttct cggccatctg cgccatttcc tggtcgagcc gcaccgtggt ctcggtcgac
     2701 tcgacgacct ccggctccgc ctgcgcccac tccagcgcct gttgcacgtc gagcgccggg
     2761 ccattggcct gcagacgctg cagctccgct gaatagatga cctcgagccg gcgaaagtcc
     2821 tcggtgtcgg cgttggccac gttggtcgcg tgcaccgtct ggcgcagcgc cgccgcgtcg
     2881 agcgcgcggc tcagcagggc aaggactgga tcggcgttca gcattggcgc actccctatt
     2941 gaatgacgag ttcggcatgc agcgcgccgg cgcgcttgat gccctgaaga atggcgatga
     3001 cgtcgcgcga gctgaccttg atcgcgcgca gcgcggcgac gaggtcggcg atggtggcgc
     3061 cgctgtcgag actgacggcc tgcgcttcgt cctcggagac ggagaccgtc gcctcgggcg
     3121 ccaccaccgt gcggatgccg ctctgccggc cgtagtagat gccttcgggt tgcgacacgt
     3181 tgtatcgctc gacgatggac acgcgcagat cgccgtgcgt gacggtgacg gccgatagat
     3241 agacgtcgcc gcccgacacc acggtgccgg tgcgttcgtt gacgaccaca cgcgcggcca
     3301 cgtcggggtc gacggccagt gattcgatgc gggccatcat cgccaccgcc gtgccggcat
     3361 cgccgttgag cgacacgcgc acgcggccgg aatcgatggc ggtggcgttg ccgccgggaa
     3421 gtgccgcgtt gattgccgcg acaacgcggg tcgcggtggt gtagtcaggc tcgtcgagca
     3481 gcagctcgag tgcgccgttg ctctgcagga tctcgggctc cagggttcgc tcggcgatcg
     3541 ccccttcggg aatcgtgccc gcggtgggaa agttcttctg cgccgacgtg ccgcgctgct
     3601 cgaacttgta gccgccgacg gccagcggcc cctgcgccat cgcgtaggac accttgttgt
     3661 tagccatgag cggcgtcatg agcaacgtgc caccggcgag gctgcgtgca tcgccgatcg
     3721 ccgcgacgtt gacgtcgaag cgatcgccgg cgcgcaccgc gccgggtaga tcggccgtga
     3781 cgatgaccgc ggcgacgttg cggctcgaca ccgcgtacgg cggcaccgcg aggccgaact
     3841 cgcgcagcat gttggacacg gactggagcg tcgtgaaact acgagccgag tcgccggtgc
     3901 cggaaagccc gacgaccagg ccgtagccaa tgatcggctg ctcgcgcact ccgtcgaacc
     3961 gcgccaagtc cttgattcgt acggctgatt gggcatccgc ggccacgatc agcgccagcg
     4021 ctatatataa gagtgtcttt gacatgggtt attggccgaa aagcgagagg agccgggtga
     4081 gaagtcccgg cgatgacttt ttcgagagca gtcccttgcc gagcaactgg atgtcggtgc
     4141 ccgcgacgcg ccacgaagga accgtgttgt cggcggcgat gtcatccggc cgcaccagcc
     4201 ccgagacgcg aatcgtctgc ttctcgttgt tgatgacgat gagctgttcg cccgagacga
     4261 tgaagttgcc gttcgaatcc acggcgtcga tgcgcaccga cagccgcgcg agcaacgcgc
     4321 ccttgcgttc gatctcgccg ccgcccgcga accggccttc gaagccggcg ttggcgagct
     4381 gcatttctcc atcccgttgc aaaaggttgg cgctggccga ctcccctttc gaggttcgcg
     4441 tctgcgcact cgccgagacc gacgccgctt cggcgatcag tacggtgatc agatcgcccg
     4501 gcatgtgcgc ccggcgatcg gcgaacagcg agcgcggcgc cgaagtagcg tccgccgcga
     4561 acgctccggg cgcgagtggc gcgagcgcca gggctaacaa gactgcggag tgttgcgttt
     4621 tcattcctgt tcctctgaaa gccgggccgc gccgggcccc gtgacgatgg cgctgaccgt
     4681 gattccgttc gtgcgatcgc ggagccgcac gcgttcaccg aagcgtgcat ccgcggcggc
     4741 ggtgagtacg cggctggcgc tgacggcgcc gcgctgtgcg ttcaaggtga caggccggtc
     4801 gcgctcgaca tccggcacga tttcgatgtc cttcgcgcac agcgcctcgc ccgcgacgag
     4861 gcgccgcgtg gcgcgccagc gctggccagc gtcgagtggt ttggccggct cgcaaccgag
     4921 cgcgatcaca tcgtgctcag cgagttccgc atcgtcggcg gtgaacgcgg ctccccgatc
     4981 gagcgcgtgc gcgctcacca gaacctggct catgcccacc acggcgtacc agcgcacgcg
     5041 tccatcggtg aaacgcaccg cggtgcgcga gccgacccgg cccacgcccg cgatgcccgc
     5101 ctccggtacc gcgttggcgc tctccgacag cggacgccac tgccaacgca gcacgtcggg
     5161 ccgctgctcg cgcagatgtg gctcgagcgc aggcgcggct tcgcccgcat gtgcgctgcg
     5221 agccacgcag accagcacga tgctggccag cgcgaaggcg cggatgagga gcgcgtgttc
     5281 catcagccgc ggtacagcga gttggtgata ccgagcatct ggtcggcggc ctggacgatc
     5341 ttgctgttca tctcgaaccc gcgctgcgcg agcatcagcg acaccaattc gtctacgagc
     5401 tgcacgttcg aggattccag gaatccctgg cgcaactggc cgttgccggc ttcgcccggc
     5461 gcgcccagct gcatttcgcc gctggcaccc gtggcctggt acaggttgtc gccgatggat
     5521 tgcaggcccg ccggattggc gaacgccgcg agttcgatgt tgccgagctc gacatcggat
     5581 cgatccgcga ccgtggccat cactcgaccg tcattcgtga tgcgcaggtc gcgcgtgtcc
     5641 gagggaaccg cgatgcgcgc gctcagtggc aggccagcac ccgcgagcat gccttcggag
     5701 ttgagcgaca gtgcgcccgc tcgggtgtac gccggcgttc catcgggcag cagcacttcg
     5761 aagaagcccg cgccgtcgat ggcgacatcg aggggcgcct gcgtctgctt gagttcgccc
     5821 gcctgcgtcg agagttgcaa cgaagcgatg gcgcccgcgc cacgcgactg cagtgtttcg
     5881 cgaagctgcg agcccagcgg atcggtcgca ccgctctgca gcgccgccat cacgctcgag
     5941 aaattcacgc tctcgcgccg gaagcccacg gtgttcacgt tggccacgtt gttggcgatg
     6001 gtgtcgacct gggtctggtg cgcgcgcatg ccggtggcgc ccacgtaaag tgcatctgtc
     6061 atggatatct cccgttaaac cttgccgagt tcgctgatcg ccttgcccat catgtcgtcg
     6121 tagcc
//
LOCUS       DATDEU010000379         5938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_799759, whole genome shotgun sequence.
ACCESSION   DATDEU010000379 DATDEU010000000
VERSION     DATDEU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799759"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..834)
                     /locus_tag="VIV63_09015"
     CDS             complement(13..834)
                     /locus_tag="VIV63_09015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6124779.1"
                     /translation="MRFDGRYRPDRPSWVVRIGAFAGLAFMHIPVLVIILYAFTTEDR
                     TYQFPPPGFTLHWFDVAFGRADIWQALSLSLGVAACATFIALVLGTFAAFALARAKFA
                     AKDSLAVLFVLPIALPGIITGIALLAAMKMARLDPSFLTIVAGHATFCTVIVYNNVIA
                     RLRRLPPSWTEASMDLGADGLQTFRYVLLPQVATAFLAGGMLAFALSFDEIIVTIFTA
                     GKERTLPIWFFNELFRPRDRPVTNVVAVIVIAVTLIPIVLAYYLTRPDDEHETAR"
     gene            complement(824..1735)
                     /locus_tag="VIV63_09020"
     CDS             complement(824..1735)
                     /locus_tag="VIV63_09020"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6124780.1"
                     /translation="MTVRAASTFFYRRPNWYLCLLLIPPLLWFGTVYLGSLFALLAQS
                     FYAIDDFTAQIVREPTLATYKQLVTQPANIDIVLRTLTMAIAVTLACAVIALPIATYM
                     ARYARGRMKAFFYVGVMLPMWTSYLVKVYAWRLLLAKEGIVAWVTGKLGLTGLLETAL
                     AAPGVGGPSLSSSYIGMFIVFVYMWLPFMILPMAAALERVPGSLLQASADLGARPAQT
                     FRNVVLPLAVPGIAAGSVFTFSLTLGDYIIPSVVGAPGYFIGMMVYVQQGTAGNIPLA
                     AAFSVVPIILIFAYLSIAKRFGAFDAL"
     gene            complement(1732..2754)
                     /locus_tag="VIV63_09025"
     CDS             complement(1732..2754)
                     /locus_tag="VIV63_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6124781.1"
                     /translation="MTAAIEFRGVSRVFGDVRAVDDVSFVIEPGEFFAMLGPSGSGKT
                     SCLRLVAGFDAPDRGQVLLEGLDVSAIPPYDRNVNTVFQDYALFPHMTVLENVAYGPR
                     VRGVDATTRAKSAREMLDLVQLGAQGERRPAQLSGGQRQRVALARALINHPKVLLLDE
                     PLGALDLKLREEMQIELKSLQRRLGITFVYVTHDQGEALSMADRVAVFNKGKIEQLAA
                     PRELYTRPGTAFVARFVGSANVAEGALARAISGSETPFAIRAENIAVQIESASATPDS
                     VSAPGAVVAVQYHGAASRWQVKLDAGEVWSALITEDESRRLNGLAVGARVRLCWPRDA
                     AVTLKS"
     gene            complement(2771..4009)
                     /locus_tag="VIV63_09030"
     CDS             complement(2771..4009)
                     /locus_tag="VIV63_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6124782.1"
                     /translation="MFSASAKRLLLVVCSATLTLAACSKKEEAPKAADAMMASTTLEG
                     ALDIVAWPGYIERGETDKAYDWVSRFEADTGCKVTVKTAGTSDEMVSLMTSGTPQSPP
                     GDASWPPAGSAPYDLVTASGDASIRLIRGGTVMPIDVARVPSYNTVDPRLQKAAWHYV
                     DGKHYGVPYQWGPNVLMYNTETFKTPPTSWSVVFEPQKLPDGKPNKGRVQAYDGPIYI
                     ADAALYLKAKKPDLGITDPYSLNEAQYAAVLEVLRGQHPLIQRYWHDANVQVQDFQNE
                     GIVASGSWPFQANTLLMNKAKVATTVPAEGATGWADSTMLATGAKHPNCAYKWLEWSI
                     SAKVQGDVAAWFGSNPVVPAACTGNELLGAEGCKTNGIENFDKIAFWRTPEAKCEASA
                     EGCVPYSRWVSDYVAIIGGK"
     gene            complement(4081..5475)
                     /locus_tag="VIV63_09035"
     CDS             complement(4081..5475)
                     /locus_tag="VIV63_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY6124783.1"
                     /translation="MSLQTKQDHRLPAQLAARLAQRIENHALRPGTRLPSIRNCAEES
                     RVSRSTVVEAYDQLIAAGLIESRRGSGFYVRLRNNKAAHRRAASHPLPGTVLDVSWLL
                     RAQRRQSPGSEQPGAGLLPEDWLDHEMVAAAVRGVGRSRGSWLLKYGAVEGYLPLREA
                     LSTRLAHAGIVVAPEQIVTTAGATQAIDLVARRFLAPGDLVFVEDPAWFVMFARFAAF
                     GARIVGVPRGVDGPDIAALESLLARERPKLFILNSVAHNPTGSCMSAEVAARVLELAT
                     RHDFMILEDDIYGDLQGRHAVRLAALDDGRRVIYVGGFAKTLAANLRVGFVAAHPALA
                     RELRDIKALTGLSSSELGERVVARIIIERSYERMLERLRVRLDDCRSRTAEALLRMGA
                     RIAGPPPGGMYLWADFGRDTNELATAGSREGVLCAPGSLFSPTQLPSNWMRVAAATCL
                     NATAMGFLQRAMRT"
     gene            5557..>5938
                     /locus_tag="VIV63_09040"
     CDS             5557..>5938
                     /locus_tag="VIV63_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY6124784.1"
                     /translation="MNAVFAATPPALPDRAAMESAAQFLATLVPPTPQYVWPQVAAAF
                     GTEVWLKHENHTPIGAFKARSAAMYFEKMMQREPHCPGVITATRGNHGQAVGLAARRF
                     KLPAIVYVPHGNSVEKNTAMRALGV"
BASE COUNT         1088 a         1928 c         1960 g          962 t
ORIGIN      
        1 accagcttgc cgttaacgag cagtttcgtg ttcatcgtcg ggcctcgtga ggtagtaagc
       61 gagcacaatc ggaatcagcg tcacggcgat gacgatgacc gccaccacgt tggtcaccgg
      121 ccggtcgcgc ggccggaaca gttcgttgaa aaaccagatg ggcagtgtgc gctctttgcc
      181 ggcggtgaat atggtgacga tgatttcgtc gaacgacaac gcgaacgcga gcatgccgcc
      241 ggccaggaac gcggtggcga cctgcggcag cagcacatag cgaaaggtct gcaggccatc
      301 cgcgccgagg tccatggacg cttccgtcca tgaaggcggc aggcgccgca accgcgcgat
      361 gacgttgtta tagacgatga ccgtgcagaa cgtcgcgtgg ccggccacga tggtgagaaa
      421 cgacgggtcg agccgtgcca tcttcatggc ggctaacaac gcgatgccgg tgatgatgcc
      481 cggcagcgcg atgggcaaca cgaacaacac cgcgagggaa tctttcgcag cgaacttcgc
      541 gcgcgcgagc gcaaacgcgg cgaacgtgcc gagtaccaga gcgatgaagg tcgcgcaggc
      601 cgccacaccg agcgacagac tcaacgcctg ccagatgtca gcccggccga acgccacgtc
      661 gaaccagtgc agcgtgaaac ccggcggcgg gaattgatag gtgcgatcct cggtcgtgaa
      721 ggcgtagagg atgatgacga gcaccgggat gtgcatgaac gcgagcccgg cgaacgcgcc
      781 gatgcgaacg acccacgacg gccggtctgg ccggtaacgc ccgtcaaagc gcatcgaacg
      841 ctccgaagcg cttggcgatc gacaggtacg cgaagatcag gatgatgggc accaccgaga
      901 acgccgccgc cagcggaata tttccggccg tgccctgctg cacgtacacc atcatgccga
      961 tgaaatagcc cggcgcgccc acgacgctcg ggatgatgta gtcgccgagc gtcagcgaga
     1021 acgtgaacac cgatcccgcg gcgatgccgg gcacggcgag cggcagcacg acgttgcgaa
     1081 acgtctgcgc aggtcgcgcg ccgagatccg cggaagcctg caacagcgaa cccggcacgc
     1141 gctcgagcgc ggcagccatg ggcaggatca tgaatggcaa ccacatgtac acgaacacga
     1201 tgaacatgcc gatgtatgag gacgacagcg acggaccgcc gacgccgggc gccgccagcg
     1261 cggtctccag caacccggtg agcccgagct tgcccgtgac ccacgcgacg atgccctctt
     1321 tcgcgagcag caggcgccac gcgtatacct ttacgaggta gctggtccac atcggcagca
     1381 tgacgcccac gtagaaaaac gccttcatgc ggccacgcgc atatcgcgcc atgtaggtgg
     1441 cgatgggcag cgcgatgacg gcgcaggcca gcgtcaccgc aatggccatg gtcagcgtgc
     1501 gcagcacgat gtcgatgttc gccggctgag tgacgagttg tttgtacgtg gcgagcgtgg
     1561 gctcacgcac gatctgcgcg gtgaagtcgt cgatggcgta aaacgattgc gccagcagcg
     1621 cgaacagcga tcccaggtag accgtgccga accacagcag cggcggaatg agcagcaggc
     1681 acaggtacca gttcgggcgt cggtagaaga acgtcgacgc cgcgcggact gtcatgactt
     1741 gagcgtcacc gccgcgtcgc gcggccagca gagcctgacg cgcgcgccca ccgcgaggcc
     1801 attcaatctg cgcgactcgt cttcggtgat cagcgcgctc cagacttctc cggcgtcgag
     1861 cttcacctgc cagcggctcg cggcgccgtg atactgcacc gcgacgaccg cgcccggcgc
     1921 gctcaccgaa tccggcgtcg ccgacgcgga ctcgatctgc acggcaatgt tttcggcgcg
     1981 aatggcgaac ggagtctccg acccgctgat cgcacgggcc agtgcgccct cggcgacatt
     2041 ggcgctgccg acgaagcgcg cgacaaaggc ggtgccaggc cgcgtgtaca actcgcgcgg
     2101 cgccgcgagc tgctcgatct tgcccttgtt gaacaccgcg acacggtccg ccatcgacaa
     2161 ggcttcgccc tggtcgtgcg tgacgtagac gaaggtgatt ccgagccggc gttgcaggct
     2221 cttgagttcg atctgcattt cctcgcgcag cttgagatcg agcgcgccca gcggctcgtc
     2281 gagcagcaac accttgggat gattgatgag tgcgcgcgcc agcgccacgc gctgacgctg
     2341 accgcctgac aactgcgcgg gccggcgttc gccttgtgcg ccgagctgta cgagatcgag
     2401 catctcgcgc gcgctcttcg cgcgagtggt cgcgtcgaca ccgcgcactc gcgggccgta
     2461 cgcgacgttc tcgagcacgg tcatgtgcgg gaacagcgcg tagtcctgga agaccgtgtt
     2521 gacgttgcgg tcatagggcg ggatggcgga gacgtccagc ccttcgagca acacctgtcc
     2581 gcggtcgggc gcatcgaagc ccgccacgag ccgcagacag gacgtcttgc ccgaacccga
     2641 agggcccagc atcgcgaaga actcgccggg ctcgatcacg aacgacacgt catcgaccgc
     2701 gcgcacgtcg ccgaacacgc gcgagacgcc gcggaattcg atcgccgcag tcatggtgcg
     2761 gtctgcggtc ttattttccg ccgattatcg ccacgtagtc gctgacccag cggctgtacg
     2821 gaacgcagcc ttccgcgctc gcttcgcact tggcttccgg cgtgcgccag aaggcgatct
     2881 tgtcgaagtt ctcgatgccg ttggtcttgc acccttcggc gcctaacaac tcgttgccag
     2941 tgcacgccgc cggaaccacc gggttcgaac cgaaccaggc ggccacgtca ccctgcacct
     3001 tggcggaaat ggaccactcg agccacttgt aggcgcagtt cggatgtttg gcgccggtcg
     3061 cgagcatggt gctgtcggcc cagcctgtgg cgccttccgc gggaaccgtg gtcgccacct
     3121 tcgccttgtt catgagcagc gtgttggcct ggaacggcca ggaacccgag gcaacgatgc
     3181 cttcgttctg gaaatcctgc acctgcacgt tggcgtcatg ccagtaacgc tggatcagcg
     3241 gatgctggcc acgcagcact tcgagcaccg cggcgtactg ggcctcgttg agcgaatacg
     3301 gatcggtgat gccgagatcc ggcttcttcg ccttgagata cagcgcggcg tcggcgatgt
     3361 agataggacc gtcgtatgcc tgcacccggc ccttgttggg cttgccgtcc gggagtttct
     3421 gcggctcgaa caccaccgac caggatgtcg gcggcgtctt gaaagtctcg gtgttgtaca
     3481 tcaacacgtt cggaccccac tggtacggca caccgtagtg tttgccgtcg acgtaatgcc
     3541 acgccgcctt ctgcaggcgc ggatcgacgg tgttgtagct gggcacgcgc gccacgtcga
     3601 tgggcatcac ggtgccgccg cggatcaggc gaatggacgc gtcacccgaa gcggtcacca
     3661 ggtcgtacgg agcggagccc gccggcggcc acgacgcatc gcccggaggg ctttgcggtg
     3721 taccgctcgt catgagcgaa accatttcgt ctgaagtgcc cgcggtcttt accgtgacct
     3781 tgcaacccgt gtcggcttcg aacctggaga cccagtcgta ggccttgtcg gtttcgccac
     3841 gctcgatgta gccgggccag gcgacgatgt cgagtgcgcc ttcgagagtg gtggaagcca
     3901 tcatggcgtc tgcggctttc ggcgcttcct ctttcttcga gcaggctgcg agcgtcaggg
     3961 tggcgctgca aacaaccagc aacaaacgtt tcgcggatgc ggagaacatg gcgagcccct
     4021 ttttgtatgc gttatcaagt ccaggcgact ttgcggacta ttctaggctt aagtccgggc
     4081 tcaagttcgc attgcgcgct gcagaaaacc catcgccgtt gcattgaggc aggtcgccgc
     4141 cgcgacacgc atccagttgc tgggaagttg cgtgggcgag aagaggctgc ccggcgcgca
     4201 caacactcct tcgcggctgc cggcggtggc gagttcgttg gtatcgcggc cgaaatccgc
     4261 ccagagatac atgccgcccg gcggcggacc ggcgatcctg gcgcccatgc gcagcagcgc
     4321 ctccgcggtt cgcgagcggc agtcgtcgag cctgacgcga agccgttcga gcatgcgttc
     4381 gtagctgcgc tcgataatga tgcgagccac cacgcgctca ccgagttcac tcgatgaaag
     4441 acccgtgagc gccttgatgt cgcgcagctc gcgcgcgagt gccggatgcg cggcgacgaa
     4501 gccgactcgc aggttggccg cgagtgtctt ggcgaatccg cccacgtaga tgacacggcg
     4561 cccatcgtcg agggcagcga gccgcaccgc gtgacggccc tgcaggtcgc cgtagatgtc
     4621 gtcttcgaga atcatgaaat cgtgacgcgt cgcgagctcg agcacgcgcg ccgccacctc
     4681 cgcgctcatg caggaacccg tggggttgtg cgccacggag ttcaggatga agagcttggg
     4741 ccgctcgcgc gccagcaggc tttcgagcgc ggcgatgtcc ggaccgtcga ctccgcgcgg
     4801 caccccgacg atacgtgcac cgaacgccgc gaagcgcgcg aacatcacga accacgccgg
     4861 gtcttcgacg aacacgaggt cgccgggcgc gaggaaacgc cgcgcgacga ggtcgatggc
     4921 ctgggtcgcg ccagcggtcg tcacgatctg ctccggcgcg acgacgatgc cggcatgcgc
     4981 cagccgcgtg ctcaaggcct cgcgcagcgg tagatagccc tcgacggcgc cgtacttcag
     5041 cagccacgaa ccgcggcttc gccccacgcc gcgcaccgcg gccgcgacca tttcgtgatc
     5101 gagccagtcc tcggggagca ggccggcgcc gggctgttcg ctgccgggcg actgccgtcg
     5161 ctgcgcgcgc aacaaccagc tcacatcgag cacggtgccg ggcaacggat gggacgccgc
     5221 gcgccgatgc gcggccttgt tgttccgcag gcgcacgtag aagccggaac cgcgacggga
     5281 ttcgatgagc cccgcggcga tcagttgatc gtaggcctcg accaccgtcg accggctgac
     5341 gcgcgattcc tcggcgcagt tgcggatcga gggaagccgg gtgccggggc gcaacgcatg
     5401 attctcgatt cgctgcgcga gacgcgcggc gagctgggcg ggcaggcggt ggtcttgttt
     5461 ggtctgcagc gacatatata agagcgggcc agtcggatgg tttgggcgga taactgtact
     5521 gcaactgtac tgagttcgtc ccgtatattt cgcgacatga acgctgtatt cgccgcgact
     5581 ccgccagccc tgcccgaccg cgccgccatg gaatccgcgg cgcaattcct ggcgacgctg
     5641 gtcccaccta ccccgcaata cgtctggccg caggtggccg cggcgttcgg cacggaagtc
     5701 tggctcaagc acgagaacca cacgcccatc ggcgccttca aggcgcgcag cgccgcgatg
     5761 tatttcgaga agatgatgca gcgcgagccg cattgcccgg gcgtcatcac cgccacgcgt
     5821 ggcaatcacg ggcaggccgt gggcctcgcg gcgcgccggt tcaaactacc cgcgatcgtt
     5881 tacgtgcctc atggcaacag cgtggagaag aacactgcga tgcgcgcgct cggcgtga
//
LOCUS       DATDEU010000380         3587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1528849, whole genome shotgun sequence.
ACCESSION   DATDEU010000380 DATDEU010000000
VERSION     DATDEU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528849"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VIV63_09045"
     CDS             <1..1261
                     /locus_tag="VIV63_09045"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6124785.1"
                     /translation="MRELLRNKPVFSWAVYDWANSAFATTVMAGFFPVFFQKFWSTGV
                     ASTVTTQRLGYANAAAGLAIALLAPVLGAIGDRGGRRKQFLMAWTLLGVGATGALYFV
                     GQGEWMPAALLFVLGTMGFNGGVVFNDSLLLDVAKPADLDRVSAFGYSLGYLGGGVLF
                     LINVLMVSKPAWFGLADASEAVRTSFITVAVWWLVFTIPLMRGVKEQERGPHLPLADA
                     VRVGFRELGATIAHVRQYRTIVMFLLAYWFYIDGVNTIIKMAVNYGVSLGLDTNSLLT
                     ALLVTQFVGFPAALFFGYLGDRIGPKRGILIGLAVYAGITVYAYFLDSVGEFYALAVT
                     VGLVQGGVQSLSRSLFGRLVPEGKNAEFFGFYNMMGKFATVLGPLLIAIVAGVTHNER
                     ASIISLVLLFVVGGVLLWRVPITRRDS"
     gene            complement(1242..3203)
                     /locus_tag="VIV63_09050"
     CDS             complement(1242..3203)
                     /locus_tag="VIV63_09050"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY6124786.1"
                     /translation="MSATARSTTATTLVFAASVLLVSPDAPVWCVAIALAATVWRLLV
                     ARGYLAPMKSFRGMRFLLGAITAVLVFAVAASFHTLNGLAAGTALLLVMGALKLVESR
                     ARRDEGIVIGVSLFLLLASVLATQSLARVPLYLLVVWGACAAIAMIADHSGALAPRAA
                     LRLSARALAMSVPLAAACFLFFPRFAGQFWALQRGQQATTGLSDEMSPGSISKLANEY
                     DPAFRVRFETDPPAQSALYWRGPVLNDFDGFTWRRNPLKYYPQVPIELLGEPVRYRIT
                     LEPTNQRWLFALDTVAASPRRDIRMVQFDRQLEMSTPVTSVLSYDATSNLETRSTGPL
                     PTLGKRHETTLPLDRNPRARALALDLRARTGSDAAFARAVLDWFRDNGLEYTLEPGVT
                     TLDSVDTTLFDSRKGFCGHFASSYAMMMRAAGVPARVVTGYLGGEWNPVGGYIVVRQS
                     DAHAWTEIWLDETGWTRIDPTAVVAPERLNRGIFDLMPESLPLTSTFLHNSALLSRLE
                     HLWDGANQWWQERVVEFNVRAQLNLLRKLGIDSPSWQHLAWGFGAALVLWIAWVSLTL
                     RRGVARTKPDRIGRAWIRATRKLARVAPARAPAEGPMEYAGRVSAQRPDLAARIDALA
                     ALYTRLRYGREATHQDVAAFEREVRNLVV"
     gene            complement(3200..>3587)
                     /locus_tag="VIV63_09055"
     CDS             complement(3200..>3587)
                     /locus_tag="VIV63_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY6124787.1"
                     /translation="TPPEAASGGNAPAVHRVGDEEWAGLREFRTGDSPRQVAWGAYAR
                     GRGLLVKTYQSPAAHHRLFDLAAVPGTQLEQRLEQLSAWIVGAHARGERYGLRLGEHT
                     LPPDSGNEHRTRCLNGLALYGSGESW"
BASE COUNT          574 a         1190 c         1215 g          608 t
ORIGIN      
        1 catgcgcgag ctactcagaa acaagccggt cttctcgtgg gcggtgtacg actgggcgaa
       61 ctccgctttc gcgaccacgg tcatggccgg attcttcccg gtgttcttcc agaagttctg
      121 gagcaccggc gtcgcttcca cggtaacgac gcagcgcctc gggtacgcga acgcggccgc
      181 gggcctcgcc atcgcgctgc tggcgcccgt gctcggtgcc atcggcgatc gcggcgggcg
      241 ccgcaagcag ttcctgatgg cctggacttt gttaggcgtg ggcgccaccg gcgctctcta
      301 tttcgtgggg cagggcgaat ggatgccggc cgcgttgctg ttcgtgctcg gcaccatggg
      361 attcaacggc ggcgtcgtct tcaacgactc gctgctgctc gatgtcgcca aacccgcgga
      421 tctcgaccgc gtttccgcct tcggttattc gctcggctac ctgggcggcg gcgtgttgtt
      481 cctgatcaac gtgctcatgg tcagcaagcc cgcgtggttc gggctcgcgg acgcgtccga
      541 ggccgtgcgg acttcattca tcaccgtcgc ggtgtggtgg ctggtgttca cgatcccgct
      601 gatgcgcggt gtgaaggagc aggagcgcgg tcctcatctg ccgctcgcgg atgccgtgcg
      661 cgtcgggttc cgggagctcg gcgccaccat tgcccatgtg cgccagtacc gcaccatcgt
      721 catgttcctg ctggcgtact ggttctacat cgacggcgtc aacaccatca tcaagatggc
      781 ggtgaactac ggcgtgtcgc tcgggctcga tacgaacagc ctgctcacgg cgttgttggt
      841 cacgcagttc gtgggatttc cggcggcgct gttcttcggc tatctcggcg accgcatcgg
      901 accaaagcgc ggcatcctca tcgggctcgc cgtgtacgcc ggcatcacgg tgtacgcgta
      961 cttcctcgac tcggtcggcg agttctatgc gctggccgtg accgtgggtc tcgtgcaagg
     1021 cggcgtgcag agcctgtcgc ggtcgctgtt cggccggctg gtgccggaag gcaagaacgc
     1081 ggagttcttc ggcttctaca acatgatggg caagttcgcg acggtgctgg ggccgctgct
     1141 catcgccatc gtcgcgggcg tcacgcacaa cgaacgcgcc tcgatcatct cgctggttct
     1201 gctgttcgtg gtcggcggcg tgctcttgtg gcgcgtgccg atcacacgac gagattcctg
     1261 acctcgcgct cgaacgcagc gacgtcctgg tgagtggctt cgcggccgta tcgcaggcgg
     1321 gtatagagcg cggcgagcgc atcgatgcgc gcggccaggt ccggccgctg tgccgacact
     1381 ctgcccgcat attccatcgg accttcggcg ggcgcccgtg ccggcgccac gcgcgccagt
     1441 ttgcgcgtgg cgcgaatcca cgcgcgtccg atacggtcgg gcttcgtgcg cgcaacgccg
     1501 cggcgcaacg tcaggctcac ccaggcgatc cacaacacca gcgccgcgcc gaagccccac
     1561 gccaggtgct gccaggacgg tgaatcgata ccgagtttgc gtagcagatt gagctgggcg
     1621 cgcacgttga attccacgac gcgctcctgc caccactggt tggcgccatc ccacagatgc
     1681 tcgaggcggc tcagcaacgc gctgttgtgc aggaacgtac tggtcagcgg cagcgactcg
     1741 ggcatcaggt cgaagatgcc gcgattcaac cgctccggcg ccacgacggc cgtgggatcg
     1801 atccgggtcc aaccggtctc gtcgagccag atctccgtcc acgcgtgtgc gtctgactgc
     1861 cggacgacga tgtatccgcc gaccggattc cattcgccgc cgaggtagcc ggtgacgacg
     1921 cgggcgggca cgccggcggc gcgcatcatc atcgcgtacg aagacgcgaa gtggccgcag
     1981 aaacctttcc tggaatcgaa cagcgtcgtg tcgacggaat cgagcgtggt gacgccgggc
     2041 tcgagcgtgt attcgagtcc gttgtcgcgg aaccagtcga gcaccgcacg cgcgaatgcc
     2101 gcatcgctgc cggtgcgcgc ccgcaggtcg agcgccagcg cgcgggcgcg cgggttgcgg
     2161 tcgaggggca gggtggtctc gtgccgcttg cccagcgtcg gcaatgggcc ggtgctgcgg
     2221 gtttcgaggt tcgacgtcgc gtcgtaactc aagacgctgg tcaccggcgt gctcatctcg
     2281 agctgccggt cgaactggac catcctgata tcgcgtcgcg ggctcgcggc caccgtgtcg
     2341 agcgcaaaca accagcgctg gttcgtcggc tcgagcgtga tgcgatatcg caccggctcg
     2401 cccagcaact cgatcggcac ctgcgggtag tatttaagcg ggttccggcg ccaggtgaat
     2461 ccgtcgaagt cgttgagcac ggggccgcgc cagtacagcg cggactgggc gggcggatcg
     2521 gtctcgaagc gcacgcgaaa cgccggatcg tattcattgg ccagcttgct gatgctgccc
     2581 ggtgacattt catcggagag accggtggtg gcctgctggc cgcgctgcaa cgcccagaac
     2641 tgaccggcaa aacgcggaaa gaacaggaaa cacgccgccg ccagcggcac ggacatggcc
     2701 agcgcacgcg ccgaaagccg cagcgcggcc cgcggcgcca gcgcgccgct gtggtcggcg
     2761 atcatcgcga tcgcagcgca ggcgccccac acgacgagta gatagagagg cacgcgcgcc
     2821 aaagactgcg tggcgagcac ggaggccagc agcaggaaca gcgacacacc gatcacgatg
     2881 ccttcgtcgc ggcgggcgcg cgattcgacc agtttcaacg cgcccatcac cagcagcagc
     2941 gcggtgcccg ccgccaggcc gttgagcgtg tggaaactcg ccgccaccgc gaacaccagc
     3001 accgccgtga tcgcgccgag caggaagcgc atgccacgga aggatttcat cggcgcgagg
     3061 tagccgcggg ccacgagcag gcgccacacc gttgcggcga gcgcgatggc gacgcaccag
     3121 accggcgcgt ccggagaaac cagcaagacg ctggccgcaa acaccagcgt ggtcgcggtg
     3181 gtcgaccgtg cggtcgcgct caccatgatt cgccgctgcc gtacaacgcc agtccgttga
     3241 ggcagcgcgt gcggtgttcg ttgccgctgt ccggcggcaa tgtgtgttca cccagccgca
     3301 acccgtaacg ttcgccacga gcgtgcgcac cgacgatcca cgccgacaac tgttcgaggc
     3361 gttgttcgag ctgcgtaccg ggcacggccg cgagatcgaa cagccgatga tgagccgcgg
     3421 gagattgata ggtcttcacc aaaaggccgc ggccgcgcgc gtaggctccc caggccacct
     3481 ggcgcggcga atcgcccgtg cggaattcgc gcaagcccgc ccattcctcg tcgcccacgc
     3541 gatgcacggc cggcgcgttg ccgccgctcg ccgcttcggg cggagtt
//
LOCUS       DATDEU010000381         3864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_360449, whole genome shotgun sequence.
ACCESSION   DATDEU010000381 DATDEU010000000
VERSION     DATDEU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360449"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..2705)
                     /locus_tag="VIV63_09060"
     CDS             complement(42..2705)
                     /locus_tag="VIV63_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY6124788.1"
                     /translation="MPITAADWKRLSPLLDVALDLTPAERAGWLAALPPEHADLRDSL
                     AEMLSRRLVETDDFLNRLPEFSAAPVALSLGPGSVVGPYRLQRELGQGGTSSVWLAEL
                     ADGSIKRSVALKLPHLGLVDRGIEQRIAREREILAGLEHPHIARLYDAGVDERGRPYL
                     ALEYVDGVPPDEYCRTEKLNLRQKLSLFLNILRAVAFAHARLIVHRDLKPNNILIAAD
                     CDVRLLDFGIARLLQPDLGVRAQHTVAGAAALTPAYAAPEQFTGQPVTVATDVYSLGV
                     ILFELLTDASPYAPAGRSLGAYEHEVLYVEPPLMSRTARPAEAGALRGDLDVIVAKAL
                     EKKPADRYASVEAFASDIERHLAAQPIAARPRSFAYVAKKFVRRNLLPLSITAATVIL
                     LSVSLGVAAWQWRDAERQRAMAVDRLANSEAAALFTSTVLIEGMQPGESLTFEQLIAR
                     SEEIARQTGRNDLRSRIFATQFLSDWYRANGMSRRAEEVLTRTIDSLPADSQRLGSTL
                     RCSRAYSWRQLGRSDEAIEALTREIALNDDDPAVAAQCMLIRSWIAANSGDSHNALDF
                     AQRALVRYEEAGVESIFTRSELLQALGGAHAIRDEFAAAHEKYREALALFNASGRGHR
                     RASAQVHDDWASVWMNAGNPRRALEEIDIAWQMTRELSPGAQATDNRIGRRARILGQL
                     AQYDEAQTEWAEALDLARVRGNRPNIAAAFIGRAEIDTLQGRFAEAQEQLQRAEAALR
                     ESALPPNHVLNTRYTLTNAALLAARGNSRGATAELSRALEQYRAQDCCRSHMALTLAL
                     RGEVALREGRVSDAMLDARSARDLAPQIADESFSRFTGRAWYVTGLVYEHQRRQREAR
                     EAFATAAVQFAGAVGEAHPDTARAREGIARN"
     gene            complement(2772..3323)
                     /locus_tag="VIV63_09065"
     CDS             complement(2772..3323)
                     /locus_tag="VIV63_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEY6124789.1"
                     /translation="MDELSLLIQRAHAGDEAARERLFAVAYEDLRGQARRQLRDGGRN
                     TLLNTTVLLHESFLRFQRAGKLAGAERGHFFAYAARVMRSVIVDFARQRQAERRGGDA
                     VHVPLDAQFAENLRAGEDEVIQINEAIEALQGEDPRLVQVVEMRYFAGMTETEIATVL
                     GVTDRTVRRDWEKARLLLAAMLR"
     gene            complement(3335..>3864)
                     /locus_tag="VIV63_09070"
     CDS             complement(3335..>3864)
                     /locus_tag="VIV63_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124790.1"
                     /translation="GALVDRYVVFSHGKDAGPWGVKITALAGNARSEGYEVESVDYRG
                     IDDPRERVNKLVEYCKELKGDLVLCGSSLGGYVSVAAASLLHARGVFLMAPALTMPGL
                     PPLRERLLDCPTMIVHGWKDDVVPVEQSMEFARRHGCALHILNSDHRLQDEVRLLKYL
                     FEYFLISLDLPQDVS"
BASE COUNT          612 a         1298 c         1310 g          644 t
ORIGIN      
        1 cccttttgct agcaaatcag gaatgtcccc attaatcccc attaattgcg ggcgatgccc
       61 tcgcgggcgc gcgcggtgtc gggatgcgcc tcacccacgg cgccggcgaa ctgcacggcg
      121 gcggtggcga atgcttcgcg cgcctctcgc tgccgtcgct ggtgctcgta gacgagcccg
      181 gtgacgtacc acgcgcggcc ggtgaagcgt gaaaacgatt cgtcggcgat ctgcggcgcg
      241 aggtcgcggg cgctgcgtgc atccagcatc gcgtcggaga cgcgaccttc ccgcagcgcg
      301 acctcgccgc gcagcgccag cgtcaacgcc atgtgggaac ggcagcaatc ctgcgcgcgg
      361 tattgctcga gcgcgcggct cagctccgcg gtggcgcccc gcgaattgcc gcgcgcggcg
      421 agcagcgctg cattcgtgag cgtgtaacgc gtgttgagca cgtgattcgg cggcagcgcg
      481 ctctcacgca gcgccgcctc ggcgcgttgc agctgctcct gcgcttcggc gaaccggccc
      541 tgcagcgtgt cgatctccgc gcggccgatg aacgcagcgg cgatgttcgg ccggtttccg
      601 cgcacgcgcg cgagatcgag cgcttccgcc cactcggttt gcgcctcgtc atattgcgcg
      661 agctgaccga ggatgcgcgc gcgacgcccg atgcggttgt cggtggcctg cgcgccggga
      721 gacagctcgc gcgtcatctg ccaggcgatg tctatctcct cgagcgcgcg gcgcggattg
      781 ccggcgttca tccacacgct ggcccagtcg tcgtgcacct gcgcggacgc gcggcggtgg
      841 ccgcggcccg acgcgttgaa taacgcgaga gcctcgcgat atttctcgtg cgccgccgcg
      901 aattcgtcgc ggatggcgtg ggcgccaccg agcgcctgca ggagctccga cctcgtgaag
      961 atggactcga cgcccgcttc ctcgtaacgc acgagcgcgc gctgcgcgaa atcgagcgcg
     1021 ttgtgcgagt cgccactgtt ggcggctatc cacgaacgaa tgagcatgca ttgagcggcc
     1081 acggccggat cgtcgtcgtt cagcgcgatc tcgcgcgtca gcgcttcgat cgcttcgtca
     1141 ctgcgcccga gctggcgcca cgaataggcg cgcgagcagc gcagcgtcga accgagtcgc
     1201 tgtgaatcgg cgggcagcga gtcgatggtg cgcgtcagca cttcttcggc gcggcggctc
     1261 atgccgttgg cgcgatacca gtcggacagg aactgcgtgg cgaagatccg cgagcgcaga
     1321 tcgttgcggc cggtctgccg cgctatctcc tcgctgcgtg cgatgagttg ttcgaacgtg
     1381 agtgactcgc ccggctgcat gccttcgatg agcacggtgg acgtgaacag cgccgcggct
     1441 tccgaattcg cgagccggtc cacggccatg gcgcgctgtc tttcggcatc acgccattgc
     1501 cacgccgcga cgcccaggct caccgacaac aggatgacgg tcgccgccgt gatgctgagc
     1561 ggcaggagat tgcgccgcac gaacttcttc gccacatacg caaacgagcg cggccgcgcc
     1621 gcgatgggct gcgcggcgag atgccgctcg atgtcgctgg cgaatgcctc gaccgacgcg
     1681 taccggtccg cgggcttttt ctcgagcgcc ttggccacga tgacgtcgag gtcgccacgc
     1741 agggcgccgg cctcggcggg tctggccgtg cggctcatca gcggcggctc cacatacagc
     1801 acttcgtgct cgtaggcgcc gagcgagcgt ccggccggcg cataggggct cgcgtccgtc
     1861 agcagctcga acaggatgac gccgagcgag tagacgtcgg tggcaacggt caccggctga
     1921 ccggtgaact gttcaggcgc ggcgtacgcg ggcgtgagcg cggcggctcc cgccaccgtg
     1981 tgctgcgcgc gcacgcccag atccggctgc aacagccggg cgataccgaa gtcgagcagg
     2041 cggacgtcgc agtcggcggc gatcagaatg ttgttaggct tgagatcgcg atgaacgatg
     2101 aggcgcgcgt gcgcgaacgc caccgcccgc aggatgttca ggaacagcga aagcttctgg
     2161 cggaggttca gcttctcggt gcggcaatat tcgtcgggcg gcacgccatc cacgtactcg
     2221 agcgcgagat agggccgccc gcgctcgtcc acgccggcgt cgtagagcct ggcgatgtgc
     2281 gggtgttcga gtccggcgag gatttcgcgc tcgcgcgcga tgcgttgctc gatgccccgg
     2341 tcgacgagtc cgaggtgcgg cagcttgagc gccacgctgc gcttgatgga accgtcggcc
     2401 agttcagcga gccagaccga cgacgtgccg ccctgcccca gctcgcgctg aagacggtag
     2461 ggcccgacga ccgagcccgg gccgaggctg agcgccacgg gcgccgcact gaactcggga
     2521 agacgattga ggaaatcgtc ggtttccacc aggcgccggg agagcatctc ggccagcgag
     2581 tcgcgtaagt ccgcgtgctc cggcggcagt gctgcgagcc agcctgcgcg ctcggccggc
     2641 gtcaggtcga gcgccacgtc gagcaaaggg ctcaatcgtt tccagtcggc ggcggtgatc
     2701 ggcatctcgt ttttctcgcg cgaatgaagt ccctgaggct acggacgcgg tgaagcgctc
     2761 gatccggaca ttcagcggag catggccgcc aacaatagcc gcgccttctc ccagtcgcgc
     2821 cgtacagtcc ggtcggtgac gcccagtacg gtggcgattt ccgtttcggt catgcccgcg
     2881 aaatagcgca tctcgaccac ctgcaccagg cgcgggtctt caccctgcag cgcctcgatg
     2941 gcctcgttga tctggatgac ttcgtcttca ccggcgcgca ggttttcggc gaactgggcg
     3001 tcgagcggca cgtgtacggc gtcgccgccg cggcgctccg cctggcgctg ccgcgcgaag
     3061 tcgacgatga ccgaacgcat gacgcgtgcg gcgtaggcga agaagtgccc gcgctccgcg
     3121 ccggccagtt tccccgcgcg ctggaagcgc agaaaactct cgtgcagcag cacggtggtg
     3181 ttcagcagcg tgtttcgccc gccatcgcgc agttgccggc gtgcctgccc acgcagatct
     3241 tcatacgcca cggcaaacag ccgctcgcgg gccgcctcgt cgccggcgtg cgcacgctga
     3301 atgaggagag acagctcgtc catgagcgag gccgctagct cacgtcctgc ggaagatcga
     3361 gcgagatcag gaagtactcg aacaggtact tgaggaggcg aacctcatcc tgcagccggt
     3421 gatcgctgtt caggatgtgc agcgcgcacc catgacggcg cgcgaactcc atgctctgct
     3481 ccacgggaac cacatcgtct ttccagccat gcacgatcat ggtcgggcaa tcgagcagtc
     3541 tttcgcgcag tggcggaagc ccgggcatcg tcaacgccgg cgccatcagg aacacgccac
     3601 gcgcatgcag caaggacgct gccgccaccg acacgtagcc gccgaggctc gagccgcaga
     3661 gcaccaggtc gcccttgagc tccttgcagt actccacgag cttgttcacg cgttcgcgtg
     3721 gatcgtcgat cccgcgatag tccaccgatt cgacttcgta gccttcgctg cgtgcgtttc
     3781 cggcgagcgc ggtgatcttc acaccccagg gtccggcgtc cttgccatgc gaaaagacta
     3841 cgtatcggtc cacgagggct cctt
//
LOCUS       DATDEU010000382         6655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2112408, whole genome shotgun sequence.
ACCESSION   DATDEU010000382 DATDEU010000000
VERSION     DATDEU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112408"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..267
                     /locus_tag="VIV63_09075"
     CDS             67..267
                     /locus_tag="VIV63_09075"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEY6124791.1"
                     /translation="MNRTLIIIGVLIALIGVLWKPLSMLPLFRLPGDFVIDRPGFKFF
                     FPLTTMILVSLVVSLILWLFRR"
     gene            complement(573..728)
                     /locus_tag="VIV63_09080"
     CDS             complement(573..728)
                     /locus_tag="VIV63_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124792.1"
                     /translation="MTKKTCAACDYPLEPSSTIKVKIGGKTVEVCCEECATKLRESAS
                     AKQKKRA"
     gene            complement(780..1652)
                     /locus_tag="VIV63_09085"
     CDS             complement(780..1652)
                     /locus_tag="VIV63_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6124793.1"
                     /translation="MSARPNPLDTPTSEFTVNRLLESGTVTVRHVDCRGTCRHRSAEE
                     CASHTHFVFPYRGVYLRHVGGDLAVADANHVLLFNQGQGYQVSHPVIGGDASLVLSVA
                     ESTMRELAPKHLVCEGADFAFNQQHLRIDARAQALVMLLKHSLQNGSIEPLEAEGLSL
                     TLVSRAIGPRTARVTGATYARRRLVDRVKVLLAGDLSQRWTLAGIAREIGGSPVYLTQ
                     VFQQVEGLPLYRYHLRLRLARALDLIAGREDLSALAQDLGFSSHSHFSAAFRQAYGRT
                     PSQFAKDFDSARSG"
     gene            complement(1714..2274)
                     /locus_tag="VIV63_09090"
     CDS             complement(1714..2274)
                     /locus_tag="VIV63_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEY6124794.1"
                     /translation="MGEITVLLNQAREGDESARERFFTRVYAELDRLARRHLSGQSPL
                     TMLDGPGLVHEVYLRLADQESLPGTDRAAFLAYASRVMRSVVIDYVRARGAQRRGGGQ
                     RMVTLNTGVVGESFTEPQLESLGDALESLDRIDERAHRVVEMRYFGGLEIEEIASFLN
                     VSPATVKRDWTKARAFLLHTMKTDCN"
     gene            2350..3027
                     /locus_tag="VIV63_09095"
     CDS             2350..3027
                     /locus_tag="VIV63_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6124795.1"
                     /translation="MKPYLPAFAALLLSVAAPALAQLGPIEPIIISGGKPGKALASIP
                     DLAEDYVIPVEVTVAADGSVKSVVVSTPSGNEAADNTAVRFMMEKKFLPAIDTEGNPV
                     EGQAQGTVEVRSKSINKVLKANMKPISTSNEVARVRKLTCKDFAWEIARLRNDGKSTM
                     LHAEVMPWVSFRLYVTDKSVAGDIEKRLLDQWPQALATAEKQCKAAPDKNYLTDVLFP
                     LLDAAAN"
     gene            3193..5142
                     /locus_tag="VIV63_09100"
     CDS             3193..5142
                     /locus_tag="VIV63_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124796.1"
                     /translation="MVYESTFYAPFAPRTALDMVNQTPGVTLDIGDDEQRGFSGAVGN
                     VLIDGERLGAKSQSLEDVLLRVPASEVLRIEILRGADVAGDASNAAVLANVVRTRTAG
                     GGTWMAGFEMTNVEKPTPTGRLAWSGRSEDREYSIGANTYSHDHISEGPREVTDGDGD
                     LVARRFGGFPHEQSEYAVNGQYSQGLGGGKIVATGQAFYSKFSEEFWLRTTTPGGSQI
                     EREIDPYNENTRSGEAGVTYQLPLSEWEMTLTALATRKHYESDVISTHFDADDVQDAE
                     FTQALRQQSGETIARGTFVRQVTGGRLEVGAEFAVNTLDGEAELTGDEGAGPVPIEVP
                     NANLSVKENRGEAFVSHAWQINPLWSLDSRLAAETSSLSFTGDTEQSVSLTYVKPRVQ
                     LTRKFGQHQLQMRVFRDVGQLDFTDFVSTASLADDIINGGNPDLRPQSEWAAQVEGDL
                     RFASDAALRVRLFHHWLDDVVDFVPVGPPGVPIDAPGNIGSGTMDGVEVSLRLPLQQM
                     LPGGTLIVGGTWRDTDVEDPLTGEHRRISDFVESEYKVELRQDLNAARLAWGISFQAL
                     TPDYDYRSAEIDSFRELRRLDAFIETTIVADTKIRLFMQNVLDDSELRDRRIYSPDRN
                     GSLALRETSNFFPEMWWMLTISGNF"
     gene            5193..5702
                     /locus_tag="VIV63_09105"
     CDS             5193..5702
                     /locus_tag="VIV63_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY6124797.1"
                     /translation="MKRTLVLTLLLIATAASAAEDKYPIKLPAPGGEVILPDAGAKKA
                     HDEWRYSAARRVGDTLYVSGVIVNRNKGEGNDVAAFKVQTRRALERLRVILEAAGSDF
                     QHVVMINSFHVWQGPNFAGTRDEQFQAFEDVIGDFLKAPYPAWTAVGTTGLLSDGGIV
                     EVQLIAKVR"
     gene            5792..6589
                     /locus_tag="VIV63_09110"
     CDS             5792..6589
                     /locus_tag="VIV63_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124798.1"
                     /translation="MTLSQAVDIRGVGGLPVLFAHSFAGDSSHWSSALDHLKPHRRAV
                     AFDFSGHGRSPPAAGHYSYDLLSRDLAAVADVVSPDPFVLVGHSMGAAVAIEYAALHA
                     QRVKALVLVDPPPAPGAIPPEQIQQIHDALENDPYAVVEQFWTQQMFIDAHPQVQQRL
                     LAALRAMPRNAVIELTQQAFAVDSTVALRRYPGPKFAIVTPRNDAPLSLHNAVPGVTH
                     AVVTGTGHWIHLDDPDGFNRTLDQFLKEKGDRSIFEKGDRSIFRGEK"
BASE COUNT         1250 a         2169 c         2040 g         1196 t
ORIGIN      
        1 atcgcaactg cgggaaaccg atgtgcgccg acatgccgcg atgcaatcgg cagttatgct
       61 ccgggcatga accggacgct gatcatcatc ggagtgctga tcgcactcat cggggtgttg
      121 tggaaaccgc tgtcgatgct gccgctcttc aggttgcctg gcgacttcgt catcgatcga
      181 cccgggttca aattcttctt tccgctgacc acgatgatcc tcgtgagcct ggtcgtctca
      241 ctgatcctgt ggttgttcag acggtaaacg ttactggatg taacccagca gaagtcccgc
      301 ggcgagaatg aaaagagtgg tgaacgttgt cggcaaatac gaaatcgacg tcacttcaat
      361 gagtccccgt gttcgcttga gtttgcggtc cggcgcttct tgcgagcgtt tcggactgcg
      421 gcgcttcagt gccggaccag tgtgcttgcc cggggtactc gcggcaatcg ggtgatccgg
      481 tagggacaag cgaaccgaga cttcttaccg ttttgattgc gcgccgccgc acggacgcgc
      541 aactcgttga acggcaagaa gtctcgtgcc ggtcacgccc gcttcttctg cttggcgcta
      601 gcggattcgc gcaacttggt cgcgcattct tcacaacaga cttcgaccgt cttgccgcca
      661 atcttcacct tgatggtgga gctcggctcg agcgggtagt cgcaggctgc gcaggttttc
      721 ttcgtcatgg cttggttcct cgggtacgcc ctcgatccgg ggcgtacgca ggaaaccatt
      781 cagccgctgc gggcgctgtc gaaatcttta gcgaactgcg agggtgtccg gccgtaggcc
      841 tggcggaatg ccgcgctgaa gtggctgtgg ctcgagaagc ccagatcctg cgcgagcgcg
      901 gacaggtctt cacgcccggc gatgaggtcg agcgcacgcg cgaggcgcag ccgaaggtgg
      961 taacgataca gcggcaagcc ctccacctgc tggaacacct gcgtgagata cacgggtgat
     1021 ccgccgatct cgcgcgcgat gcccgccagc gtccagcgct gcgacaggtc gccggccagc
     1081 aacaccttca ccctatctac cagcctgcgc cgcgcatacg tcgcacccgt gacgcgcgcg
     1141 gtccgcggac cgatggcgcg cgacaccagc gtcagcgaca acccttccgc ttcgagcggc
     1201 tcgatgctgc cgttctgcag agagtgcttg agcaacatga ccagcgcctg cgcccgcgcg
     1261 tcgatgcgca gatgttgctg gttgaacgcg aaatccgcgc cttcgcagac gaggtgtttc
     1321 ggcgcgagct cgcgcatcgt ggattcggcc acgctgagca cgaggctcgc gtcgccaccg
     1381 ataaccgggt ggctcacctg gtaaccctgt ccctggttga acagcaacac atgattggca
     1441 tcggccaccg cgagatcgcc acccacgtgc cgtagataga ccccgcgata cggaaacacg
     1501 aagtgcgtgt gcgaggcaca ctcctccgcg ctgcgatggc gacaggtgcc gcggcaatcc
     1561 acgtggcgca ccgtgaccgt cccactttcg agcaggcggt ttaccgtgaa ttcgctcgtc
     1621 ggcgtgtcga gaggattggg gcgggcggac atacgtggag agtatgtatc acccggcctc
     1681 gattccagaa aatggggaca ttcctcattt tcgtcagttg cagtctgttt tcatggtgtg
     1741 cagcaggaaa gcgcgcgcct tggtccagtc gcgtttgacc gtggccggcg acacgttgag
     1801 gaagctcgcg atctcctcga tctcgagccc cccgaagtaa cgcatctcca ccacgcggtg
     1861 cgcacgttca tcgatgcggt cgagcgattc gagcgcatcg cccagggatt cgagctgcgg
     1921 ttccgtgaag gactcaccca ccacgcccgt attcagcgtg accatgcgct ggccgccgcc
     1981 gcggcgctgg gcgccacggg cgcgcacgta gtcgatgacc acgctgcgca tgacgcgcga
     2041 ggcataggcg agaaaggccg cacggtcggt gccgggcagc gattcctggt cggccagccg
     2101 gaggtagacc tcgtgtacca gcccggggcc gtcgagcatg gtgagcggcg actggccgga
     2161 gaggtggcga cgggccaggc ggtcgagctc cgcatacacc cgggtgaaga aacgctcccg
     2221 cgccgattcg tcgccctcgc gggcctggtt cagaagtacg gtgatttcgc ccatcgggca
     2281 tcgaaattac aaggtccgac cggggcgcgt ctatctgcga aatatgtccg ccggtaacat
     2341 acgcgccgca tgaagccgta ccttcccgcc tttgccgccc tgttgctgtc cgtcgccgct
     2401 ccggcgctcg cgcagctcgg ccccatcgag cccatcatca tttcgggtgg caagcccgga
     2461 aaggcgctcg ccagcatccc cgacctggcc gaggactacg tcattccggt cgaagtcacg
     2521 gtggccgccg acggttccgt gaaatcggtg gtagttagca cgccgagcgg caacgaagcg
     2581 gcggacaaca cggccgtgcg attcatgatg gagaagaagt tcctgcccgc catcgatacc
     2641 gaaggcaacc cggtggaagg ccaggcccag ggcacggtgg aggtgcgcag caagtccatc
     2701 aacaaagtgc tcaaggccaa catgaagccg atcagcacca gcaacgaagt cgcgcgcgtg
     2761 cgcaagctca catgcaagga cttcgcgtgg gagatcgcgc gactgcgcaa tgacggcaag
     2821 agcacgatgt tgcacgccga agtcatgcca tgggtctcgt tccgccttta tgtcaccgac
     2881 aaatcggtcg cgggcgacat cgagaagcgg cttctcgacc agtggccgca ggcgttggcc
     2941 actgccgaga agcagtgcaa ggcggcgccg gacaagaact atctgactga cgtgcttttc
     3001 cctctgctcg acgcggcagc gaactgacgg cgaactttct tcgggttcgc ccacctaacc
     3061 gggactcgct aacaagccgt cgataacgga gatcaggtca tcaattcctt caaggcactg
     3121 cgctatctgg cgctcccgct cctttgcggc gatgcaatcg cgcaggctgt tcctgccgcg
     3181 tcatcggaac gcgtggtgta cgaatccacg ttttacgcgc cgttcgcgcc gcgtacagcg
     3241 ctcgacatgg tcaaccagac tcccggggtc acgctcgaca tcggcgacga cgaacagcgc
     3301 gggttttcgg gcgcggtcgg caacgtgctc atcgacggtg aacgcctggg cgcgaagagt
     3361 cagtcgctcg aagacgtgct gctgcgcgtg ccggcgagcg aggtgttgcg catcgagatc
     3421 ctgcgcggcg ccgacgtggc gggcgatgca tccaacgccg cggtgctggc caatgtcgtg
     3481 cgcacccgca ccgccggcgg cggcacgtgg atggccggtt tcgagatgac caacgtcgaa
     3541 aagccgacac ccaccggccg gctcgcgtgg agcggccgca gcgaagaccg cgaatattcc
     3601 atcggcgcca atacgtacag ccacgaccac atttcggaag gcccgcgcga ggtcactgac
     3661 ggcgatggcg acctcgtcgc acggcgtttc ggcggctttc cgcatgaaca gtccgaatac
     3721 gccgtcaacg gccaatattc gcagggtctc ggcggcggca agatcgtggc cacgggccag
     3781 gcgttttatt cgaagttctc cgaggaattc tggctgcgca ccacgacgcc cggcggctcg
     3841 cagatcgaac gcgagatcga cccctacaac gagaacacgc gcagcggcga agccggcgtc
     3901 acctatcaac ttccgctcag tgagtgggag atgacgctga ccgcgctcgc gacgcgcaaa
     3961 cattacgaga gcgacgtcat ctccacgcat ttcgatgccg acgacgtgca ggacgccgaa
     4021 ttcacgcagg cgctgcgcca gcaaagcggc gagaccatcg cgcgcggcac gttcgtgcgc
     4081 caggtgacgg gcgggcgcct cgaagtgggc gcggaattcg cggtgaatac gctcgatggc
     4141 gaagccgagc tcaccggcga cgaaggcgcg ggacccgtgc ccatcgaagt gcccaacgcc
     4201 aacctgagcg tcaaggaaaa tcgcggcgag gcgttcgtca gtcacgcatg gcagatcaat
     4261 ccgttgtggt cgctcgattc ccggctcgcc gcggaaacct ccagcctgag tttcaccggc
     4321 gacaccgaac aatccgtttc gctgacgtac gtgaagccgc gcgtgcagct cacgcggaag
     4381 ttcggccagc atcagttgca gatgcgcgtg ttccgcgacg tcggccagct cgacttcacc
     4441 gacttcgtct ccaccgcgag cctcgccgac gacatcatca acggcggcaa tccggatctg
     4501 aggccgcagt cggaatgggc cgcgcaggtc gagggcgatc tgcgtttcgc gagcgacgcg
     4561 gcgctgcgcg tgcgcctgtt ccaccactgg ctcgatgacg tcgtcgattt cgtgcccgtg
     4621 ggtccgcccg gcgttcccat cgatgcgccc ggcaacatcg gcagcggcac catggatggc
     4681 gtcgaggtct cgctgcggtt gccgttacag cagatgctgc ccggcggtac gctcatcgtg
     4741 ggcggcacat ggcgcgacac cgacgtcgag gatccgctca ccggcgagca tcgccgcatc
     4801 agcgacttcg tcgaaagcga atacaaggta gagctgcgcc aggatctcaa tgccgccaga
     4861 ctcgcgtggg gtatctcgtt ccaagccctg acgcctgact acgactatcg ttcggcggag
     4921 atcgacagct tccgcgagtt gcgccgtctc gatgcgttca tcgaaaccac gatcgtcgcc
     4981 gacaccaaga tccgcctgtt catgcagaac gtgctcgacg attccgaact gcgcgaccgg
     5041 cgcatctact cacccgaccg caacggcagt ctcgccttgc gcgagaccag caacttcttc
     5101 cccgagatgt ggtggatgct caccatctcc ggcaacttct gaatgtaaat ggggacactc
     5161 cctatttcct agcaaaaggg gtcgcacctg ccatgaaacg cacactcgtc ttgacgctcc
     5221 tcttgattgc caccgcggcc agcgccgctg aagataaata ccccatcaaa ctacccgcgc
     5281 caggcggcga agtgatcctg cccgatgccg gtgccaaaaa ggcacacgac gaatggcgct
     5341 attcggccgc gcgccgcgtc ggcgatacgc tctatgtgtc gggtgtcatc gtcaaccgca
     5401 acaagggcga aggcaacgac gtcgctgctt tcaaggtaca gacgcgacgc gcgctcgagc
     5461 gtctgcgcgt gatcctcgaa gccgcgggca gcgacttcca gcacgtcgtc atgatcaaca
     5521 gcttccatgt gtggcagggc cctaacttcg ccggcacgcg cgacgaacag ttccaggcgt
     5581 tcgaggacgt catcggcgat tttctgaagg cgccctaccc ggcgtggacc gcggtcggca
     5641 ccacgggcct tctctccgat ggcggcatcg tcgaagtgca gctcatcgcc aaggtcaggt
     5701 aggcgcgccc ctgaagcggg cactgttccc gtctagatag cggcaaacgc cgacagattt
     5761 ctcccgggcg cgtgaggcaa cgtgcgcggc catgacactt tcccaggctg tcgacatccg
     5821 gggcgtgggc ggactgcccg tgttgttcgc gcattcattc gcaggcgatt cgtcgcactg
     5881 gtcttccgcg ctcgaccatc tgaaaccgca tcgccgcgct gtcgcgttcg atttcagcgg
     5941 gcacgggaga tcgccgcccg cggccggcca ttattcctac gacttgctga gccgggatct
     6001 cgccgccgtc gccgatgtgg ttagtcccga tcccttcgtc ctcgtgggcc acagcatggg
     6061 cgcagccgtc gccatcgaat acgccgcgct tcatgcgcaa cgcgtgaagg ccctggtgct
     6121 cgtcgatcct cccccggccc caggcgccat tccgccggaa cagatccagc agattcacga
     6181 cgcgctcgag aacgatccct atgccgtggt cgagcagttc tggactcagc agatgttcat
     6241 cgacgcgcat ccccaggtcc aacagcgcct gctggctgcg ttgcgcgcga tgccgcggaa
     6301 cgccgtcatc gaactcaccc aacaagcctt cgcggtcgac tcgaccgtcg cgcttcgtcg
     6361 ctaccccggc ccgaagttcg caatcgtcac gccgcgcaac gatgcaccgc tgagcctcca
     6421 caacgccgtt ccgggcgtga ctcacgccgt cgtcaccggt accggacact ggatccacct
     6481 cgatgacccg gatggattca accgtaccct cgaccaattc ctgaaggaaa aaggggacag
     6541 atctattttt gaaaaagggg acagatctat tttccggggc gaaaaataga tctgtcccct
     6601 ttttcaaaaa tacatccgcc ccctctttcc gctgacgatc ataccctcgc cttaa
//
LOCUS       DATDEU010000383         3479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_509183, whole genome shotgun sequence.
ACCESSION   DATDEU010000383 DATDEU010000000
VERSION     DATDEU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509183"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="VIV63_09115"
     CDS             complement(<1..87)
                     /locus_tag="VIV63_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY6124799.1"
                     /translation="MAKGQSLQDPFLNTLRKERIPVSIYLVNG"
     gene            complement(159..1103)
                     /gene="miaA"
                     /locus_tag="VIV63_09120"
     CDS             complement(159..1103)
                     /gene="miaA"
                     /locus_tag="VIV63_09120"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY6124800.1"
                     /translation="MGADTKPPIAVLTGPTGTGKSDIALRLAREFPIEIVSVDSAQVY
                     RGLDIGSAKPDAATRAAVRHHLLDLVDANAAYSAGQFVRDAADAIADIEKRGRMPLLV
                     GGTMLYLRGLIGGIATLPKASEAIRAQIDADAQRLGWAAMHMRLASVDAAAAVRIHPN
                     DAQRIQRALEVHAVEGRPISELQTLTTPSLDREFLVTALVPTDRARLHAGLAQRFEAM
                     MAAGLLEEVRTLYARGDLTAENPAIRAVGYRQLWSHLEGACSLETAVERAVAATRQLA
                     KRQMTWLRSMTNIRTVDPYDAQGFVGVRESLIAAFCLL"
     gene            complement(1093..2853)
                     /gene="mutL"
                     /locus_tag="VIV63_09125"
     CDS             complement(1093..2853)
                     /gene="mutL"
                     /locus_tag="VIV63_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY6124801.1"
                     /translation="MPIRILDNHLIDQIAAGEVIERPASIVKELVENSLDAGAQSVEV
                     EIEAGGVRLTRVRDDGCGIPAAELKLAMSRHATSKISHSDDLGAITTLGFRGEALPSI
                     ASVSRFEITSRHVHEDRASSLTVDAGQLSDPSPAAHPTGTTIEVRDLFYNLPARRKFL
                     RSEVTEQGHIVRLLERLALSRDDVAFRLRSGSRTLLDAPALTATGGAASRLARIVGHE
                     FVERAIELDHSAGPVRISGWIGAPSAARATSDMQFWFVNGRAVRDRLLMNAVRLGYRD
                     VLYSGRQPAYVLYLDIDPVLVDVNAHPQKLEVRFRDSRQIHDFVFRAVERKLAGTHPG
                     SSVVPPAYTPGNQYTQPMTLTSGDSAAPGGVWAIADALRGVSPDPYPHDVEPVSMRAD
                     GAQPLGTALAQVYGIYILAQNEQGLVLVDMHAAHERVLYEKLKEQAGASVPTQSLLVP
                     VTVEMKVDELDALMAQREEWHAAGFDIERLAPEMLVVRSVPAMLPADDIPALVREVVG
                     DVASDSASHHLDSATDRLLGTIACRSAIHAHRRLTLPEMNALLRQMEQTPRADQCNHG
                     RPTWTQVSMLELDRMFLRGR"
     gene            complement(2992..>3479)
                     /locus_tag="VIV63_09130"
     CDS             complement(2992..>3479)
                     /locus_tag="VIV63_09130"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY6124802.1"
                     /translation="PDVSGSSVYVLSDRGASSEAARWLAERENAADLKGGIKLDDKDA
                     VLANVLLDLSQTASISASMVAAENILKSLDQIGEVRKPRVQQAGFVVLKSPDIPSMLV
                     ETAFISNKEDERKLSLPAHRAKLANAIFAGIEQYFQGNAPDGTRLAASRRGAGARVEL
                     R"
BASE COUNT          580 a         1130 c         1151 g          618 t
ORIGIN      
        1 tccattcacc agatagatcg acaccgggat tcgttccttg cgcaaagtat tcagaaacgg
       61 atcctgcagt gactggccct tggccatgac gttctcctta tttttcaagc aagccgacgg
      121 ccgcgagcat atcacggggt cttcgcccgg caacgcactc aaaggaggca gaaagccgcg
      181 atcagggatt cgcggacgcc tacaaaccct tgcgcatcat agggatcgac ggtgcgaatg
      241 ttcgtcatcg aacgcaacca ggtcatctgt cgtttggcga gttgacgagt ggcggcgacc
      301 gcgcgttcga ctgccgtttc gagcgaacag gcgccttcca gatgcgacca gagctgacgg
      361 tagcccaccg cgcgaatcgc cgggttctcg gcggtgagat caccgcgcgc atataacgtg
      421 cggacttcct ctagcagacc ggccgccatc atggcttcga aacgctgtgc gagtcccgcg
      481 tgcaggcgcg cacgatcggt gggaacgagc gcggtgacga ggaactcgcg gtcgagcgac
      541 ggcgtcgtca gcgtctgcaa ttccgagatg ggccgccctt ccaccgcatg gacttcgagc
      601 gcgcgctgga tccgttgcgc gtcattgggg tgaatgcgga ccgcggcggc cgcatcgacc
      661 gaggcgaggc gcatgtgcat cgcggcccag ccgagacgct gcgcgtcggc atcgatttgc
      721 gcgcgaatcg cttcgctcgc tttcggcagt gtcgcgatgc cgccgatcag cccgcgcagg
      781 tacagcatcg ttccacctac cagcagcggc atgcgcccgc gcttctcgat gtcggcgatg
      841 gcgtccgccg cgtcgcgcac gaactgcccc gcggaatacg cggcgttcgc atcgacgagg
      901 tcgagcaggt ggtggcgcac ggccgcgcgg gttgccgcgt ccggcttggc gctgccgatg
      961 tcgagcccgc gatacacctg cgccgagtcg acactaacta tctcgatggg aaattcgcgc
     1021 gcgagcctga gtgcgatgtc gctcttgccc gtgcccgtcg ggcccgtgag caccgcgatg
     1081 ggcggcttgg tgtcagcgcc cacgcaggaa catgcggtcg agctcgagca tgctgacctg
     1141 cgtccaggtc ggccgcccgt gattgcactg gtcggcgcgc ggcgtctgct ccatctgccg
     1201 cagcaacgca ttcatttccg gaagtgtgag ccgccggtgc gcgtggatcg cggagcggca
     1261 ggcgatggtg cctaacaacc ggtcggtggc gctgtcgagg tggtgactgg cggagtcgga
     1321 tgcgacgtcg ccgaccactt cgcgtaccag cgcgggaata tcgtcggcgg gcaacatcgc
     1381 cggaaccgaa cgcaccacca gcatctcggg tgcgagacgc tcgatgtcga aaccggcggc
     1441 gtgccactcc tcgcgctgcg ccatcagcgc gtcgagttca tccactttca tttcgacggt
     1501 gaccggcacc agcaacgact gcgtgggcac gctcgcgccc gcctgctcct tgagcttctc
     1561 gtacagcacc cgctcatgcg ccgcgtgcat gtcgaccagc accagtccct gctcgttctg
     1621 cgccaggatg tagatgccat acacctgcgc gagcgccgtg cccagcggct gcgcgccatc
     1681 ggcgcgcatg gaaacgggtt ccacgtcgtg tggatacgga tccgggctca cgccccgcaa
     1741 ggcatcggct atggcccaca cgcctccggg cgcggcgctg tcgcccgatg tcagcgtcat
     1801 cggttgcgtg tactgattgc ccggcgtgta tgccggcggt acgacgctcg aacccgggtg
     1861 cgtgccggcg agttttctct cgacggcgcg gaatacgaag tcgtggatct gccggctgtc
     1921 gcggaagcgc acttccagct tctgcgggtg cgcgttcacg tcgacgagca ccggatcgat
     1981 gtcgaggtag agcacgtacg cgggttgccg tcccgaatac agcacgtcgc gatagccgag
     2041 ccgcacggcg ttcatcagca gccggtcgcg caccgcgcgg ccattgacga accagaactg
     2101 catgtcggag gttgcgcgcg cggccgacgg cgcgccaatc cagcccgaga tgcgcaccgg
     2161 cccggccgaa tgatcgagct cgatcgcgcg ttcgacgaat tcgtgcccga cgatgcgcgc
     2221 gaggcgcgag gcggcgccac cggtcgcggt cagtgccggt gcgtctaaca aggttcgcga
     2281 accgctgcgc aggcggaagg cgacgtcgtc gcgtgacaac gccagccgct cgagcagccg
     2341 cacgatgtgt ccctgctcgg tgacctcgct gcgcaggaac ttgcgccgtg ccggcaggtt
     2401 gtagaacaga tcgcgcactt cgatggtggt gccggtggga tgcgccgcgg gtgacgggtc
     2461 gctcaactgg cccgcgtcga cggtcagcga cgaggcgcga tcctcgtgca cgtgccgcga
     2521 agtgatctcg aagcgcgaca ccgaggcgat ggacggcagc gcttcgccgc gaaaccccag
     2581 cgtcgtgatg gcgccgaggt cgtcggaatg cgagatcttg ctggtggcat ggcgcgacat
     2641 cgcgagcttg agttcggccg ccggaattcc gcagccgtcg tcgcgaacgc gcgtgagacg
     2701 caccccgccc gcttcgattt caacctcgac gctctgcgcg ccggcatcga ggctgttctc
     2761 caccagctcc ttgacgatgg atgccggccg ctcgatgacc tcgccggcag cgatctggtc
     2821 gatgaggtga ttgtcgagga tgcggatcgg catggccgcg cagtctaaag ggcgaaaaaa
     2881 ggggacgggc gaaaaaaggg gacagatcta tttttcgaca tgggcctgcg tcaattggca
     2941 gttgccgtgc gaaaaataga tctgtcccct tttttcccct tttttcgccc tctagcgcag
     3001 ttcgactcgt gcgccggcgc cgcggcgcga ggccgcgaga cgcgtgccgt cgggtgcatt
     3061 gccctgaaag tactgctcta ttccggcgaa gatggcgttc gcgagcttgg cgcgatgcgc
     3121 cggcaacgac agcttgcgct cgtcttcctt gttggagatg aacgcggtct cgaccagcat
     3181 cgaggggata tccggcgact tcagcaccac gaagcccgcc tgctgcacgc gcggcttgcg
     3241 cacttcgccg atctggtcga gtgatttcag gatgttttcc gctgccacca tgctcgcgga
     3301 gatgctcgcg gtctgcgaca ggtcgagcaa cacgttcgcg agcacggcgt ccttgtcgtc
     3361 gagcttgatg ccgcccttca agtcggctgc gttctctcgc tcggccaacc agcgcgcggc
     3421 ttcgctcgag gcgccgcgat ccgacaacac gtacaccgaa gatcccgaga catcgggat
//
LOCUS       DATDEU010000384         2800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1675074, whole genome shotgun sequence.
ACCESSION   DATDEU010000384 DATDEU010000000
VERSION     DATDEU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675074"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1516
                     /gene="bioA"
                     /locus_tag="VIV63_09135"
     CDS             158..1516
                     /gene="bioA"
                     /locus_tag="VIV63_09135"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY6124803.1"
                     /translation="MSGDDIRRWDAEHVWHPFTQMREYLSAEPIVVVRAEGHYLIDDR
                     GRRLFDGTSALWCNLLGHRVPEIDAAVTEQLGRFAHTTLLGTTHPGVAELARRLVEVA
                     PAGLNHVFFSDSGATAVEAALKMAFQYRLLTQGKEAAQHAVYLSLENAYHGDTLGAAA
                     VGGVELFHDIFKPILLPSLKTPSPYCYRCPIGKTPADCSIDCADELEHALRSANGRVC
                     AVILEPGVQAAAGMLTLPEGFLSRAADACRRHGALLILDEVATGFGRTGTMFACEREH
                     VAPDLMCVAKGLTGGYLPVAATLASDRVYDAFLGRYDEYRHFFHGHTYTGNALGCAAA
                     LATLGLLSDGVIINAVEQKARVLRDALAPLATHRHVGDIRQAGLMCGIELVADRTTKM
                     LFPPGDRNGYHVCLALRDRGVFLRPLGDVIALMPPLSSTDSELRHLAASVHAAIVERL
                     GA"
     gene            1509..2165
                     /gene="bioD"
                     /locus_tag="VIV63_09140"
     CDS             1509..2165
                     /gene="bioD"
                     /locus_tag="VIV63_09140"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY6124804.1"
                     /translation="MRDLLVTGTDTGVGKTVIAAAIVTALRARGVRAIGFKPVETGLT
                     AGVEPDSDVLARASGERNVLAEPLLQLPEALAPAVAADRAGAALDPDAVEARVRQLRR
                     DGYTVVVEGAGGLLVPLTWEFTVLDLAQSCDLDAVVVARPGLGTLNHVAMTVMVLRSR
                     EIPIRGIVLNGRSATPDLAESTNPAALSRMLHGVRVLEVPNSAGTPVMEAAQIVSRLL
                     "
     gene            2461..>2800
                     /locus_tag="VIV63_09145"
     CDS             2461..>2800
                     /locus_tag="VIV63_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124805.1"
                     /translation="MGVTVLAVPTVAYAQSIPNAKLDESLRESVERGCAGTQSVIVTA
                     KPGYRQSLRDSLAAHGDVVKGEFPSLEAIAADVHCEDLATLAGFDSTTSVSLNGPIAG
                     QDLPSTTTTVA"
BASE COUNT          482 a          860 c          897 g          561 t
ORIGIN      
        1 ggattcgacg tgcgggcgat tcggccgccg tcggtgccgg cgggcaccgc gcgcctccgc
       61 gttactgttc gctacccagt gagcgagggc gacctgctcc gcttcgcttc cgccatcgct
      121 ccgcttacga aaaacgtaaa aagagggtct gacccctatg tccggtgacg atatccggcg
      181 ctgggatgcg gagcatgtgt ggcatccctt tacgcagatg cgcgaatacc tttcggccga
      241 gccgattgtc gtggttcgcg ctgagggtca ctacctcata gacgatcgcg gcaggcgact
      301 gttcgacggc acctccgccc tgtggtgcaa ccttctcgga catcgcgtcc cagaaatcga
      361 tgccgcggtc accgaacaac tcgggcgatt cgcccacact acgctgcttg gcacgacgca
      421 cccgggtgtg gcggaactgg cgcggcggct ggtcgaggtt gccccagccg gactgaatca
      481 cgtcttcttc tcagacagcg gcgcaaccgc ggtcgaggcg gcgctgaaga tggcgttcca
      541 gtaccgcctg ctcacgcaag gcaaggaagc cgcccagcac gcggtgtatc tcagcctgga
      601 aaacgcgtat cacggcgaca cgctcggtgc cgcggctgtt ggcggcgtcg agctcttcca
      661 cgacattttc aagccgattc tgcttccgtc gctgaagacg ccgtcgcctt attgctatcg
      721 ctgtccgatc ggcaaaacgc cggcggattg ctcgatcgat tgtgccgacg agctcgaaca
      781 cgcgctgcgg agcgcgaatg gccgggtgtg cgccgttatc ctcgagccag gcgtgcaagc
      841 tgccgcgggc atgctgacgt tgccggaggg ttttctgtcg cgcgcggctg acgcgtgccg
      901 caggcatggt gcgctgctga tcctcgatga ggtcgccacc ggcttcggcc gcacgggcac
      961 catgttcgcg tgcgagcggg aacatgtagc gccagatctg atgtgcgtgg cgaaagggct
     1021 gacgggaggg taccttcccg ttgcagcgac gctggcgtcc gatcgcgtct acgacgcgtt
     1081 tctcggccgc tacgatgagt accgccattt ctttcacgga cacacgtaca caggaaacgc
     1141 gctcggctgt gccgccgcac tcgcgacact cgggctgctg tcggatggcg tcatcatcaa
     1201 tgcggttgag cagaaggcgc gggtgctgcg cgacgccctc gcgccacttg cgacgcaccg
     1261 ccatgtgggc gatatccgtc aagcggggtt gatgtgcggc atcgagctgg tcgccgacag
     1321 gacgaccaag atgttgttcc cgcccggcga tcgcaacggc taccacgtat gtctggcact
     1381 tcgcgaccgc ggcgtgtttc tgcgcccgct cggcgacgtg atcgccctga tgccgccgct
     1441 ctcgtcgact gactccgaac ttcggcatct tgccgcgtca gtgcacgcgg cgattgtgga
     1501 gcggctcggt gcgtgatctg ctggtgacgg gaaccgacac tggagttggg aagacggtga
     1561 tagcggcggc gattgttacc gcactgcgtg ctcggggagt gcgggcgatc ggattcaagc
     1621 cagtggaaac cgggctcacg gcaggagtgg agcccgattc tgacgtgctt gcgcgcgcgt
     1681 cgggtgagcg caacgtgctc gccgaaccgc tgctgcaact gccggaagcg ttggcgccgg
     1741 ccgtggcggc cgaccgtgcc ggagcagcgc tcgatcccga tgcagttgaa gcgagggtcc
     1801 gtcaactgcg gcgtgacggc tacacagtcg tcgtcgaggg tgccggcggg ttgctggtgc
     1861 cgctcacgtg ggaattcaca gtgctcgatc ttgcacagtc ttgcgatctg gacgccgtgg
     1921 tggtggcgcg gcccggcctc ggcacgctga accatgtggc gatgaccgtc atggtgctgc
     1981 gctctcgaga gataccgatt cgcggcatcg tcctgaatgg tcgaagtgca acaccggacc
     2041 tggcagagtc gacaaatcct gccgccctga gccgcatgct ccatggcgtt cgtgtgttgg
     2101 aggttccgaa ttcagctggg acgcccgtga tggaagcggc gcagatcgtt agcagactgc
     2161 tctgattccg aatgtgtacc tctctgcctc gaaatggcac accccgcaat tcagcgattt
     2221 cccagcaaga accccggtac gcagaaaccg atcacattag tgcgcattgg cgtgttccgt
     2281 tatgcactaa tccaccctta ctgggtcaaa gagtcgtatc cctgcacggt ttcataaccc
     2341 acactgtttc aagaggttac gtctgtgttg tatagcgtct ggtcactttg gcagtggcct
     2401 tgctctaaag gctgccgcat gactactcga ctcactcgta tcaaacacat agcgatggcc
     2461 atgggcgtca ccgtcctggc tgttcctact gttgcctacg cgcagtcgat ccctaatgcg
     2521 aagctcgatg aatcgcttcg ggaatcggtt gagcgtggct gcgcaggaac gcagtcggtc
     2581 atcgtcacgg ccaagccggg atatcggcag tcgcttcgag attcgcttgc agcgcatggc
     2641 gatgtcgtca aaggcgagtt tccctcgctc gaagccatcg ccgccgatgt gcattgtgaa
     2701 gatcttgcga cgctggccgg gttcgactcg acaacgtcgg tatcgctgaa tgggccaatc
     2761 gccgggcagg acctgcctag caccacaaca actgttgcca
//
LOCUS       DATDEU010000385         3732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1530069, whole genome shotgun sequence.
ACCESSION   DATDEU010000385 DATDEU010000000
VERSION     DATDEU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530069"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1823)
                     /locus_tag="VIV63_09150"
     CDS             complement(<1..1823)
                     /locus_tag="VIV63_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124806.1"
                     /translation="MDSKVFKDGRRPWRSLLSIGNARRSVAGKLMVVMLVTTAIALSS
                     AGAALLYTDLRDNRAAWAEDLRTEAAILALGVMPALSFNDREYAQRSLSALQARDSIH
                     AAALYAEDGSLFAQYVRADQPPPPANRPPMPGNSGLSIDGGHVLVMKNVVQGGEVLGT
                     IYLSAQYDVQGRVEAYLNVLGAVMIIGLIAALLASSWLHRVVTRPMESMAKVAQAIIE
                     KRDYSVRAEKMTDDEVGVVIDAFNKMLDEVQSHARALETSEKLYRAIGESINYGVWVT
                     DADGRCIYASDSFLRLVGLSMQQAANDGWGSVMHPDDLEETMAAWKECARTGNTWYRE
                     HRMMGVDGKYHSILAQGLPIRDADGKIQRWAGINLDISRLKNTERALLEADRRKDEFL
                     ATLAHELRNPLAPIRNAVRILDSDAADDRQRKWGREVIARQVQRMSLLLDDLLDVSRI
                     TRGQLELKKDYVDLKSVIGIAVETARPLLDAKQHKLTVKLPPENIKLEADPLRLSQVI
                     GNLLTNAAKYTDPEGHIALWSRIENGELVIGIRDSGIGLTQESLPGLFTMFSQVNSAI
                     DRAEGGLGIGLALVKGLVALHGGRVEARSEGLGRGSEFIVHL"
     gene            1929..2534
                     /locus_tag="VIV63_09155"
     CDS             1929..2534
                     /locus_tag="VIV63_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY6124807.1"
                     /translation="MIAVASFSASLVSAAQFSVDAVKAAYLFRFAQYVEWPDTAPRDA
                     PFVVAVIGAEDVAVHLERLLPGMNVNGRPTVVRRVTRAQELDGVHILFIGADAFGRTR
                     ALRARAVEMPILIVTEDQNGIDGGGVINFIEVNRNLRFEISLNAADRSGLRINSALLS
                     VAARVERRPQGAVQCLDRHPSQQRSAVCRIRMASHQSRKGA"
     gene            2531..>3732
                     /locus_tag="VIV63_09160"
     CDS             2531..>3732
                     /locus_tag="VIV63_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6124808.1"
                     /translation="MKIVCRCIVISLILAASGMPANAQLVADNSDPLTELSRMSLEQL
                     SNVEVTSVSKSAQTLSSAAASIYVITREEILRSGVLSVPEALRLAPNLQVTQLTSSEY
                     SNGARGFAGAPDVQNFSNKILILIDGRSVYSPLFSGVSYDMLDVVMDDIDRIEVISGP
                     GATLWGANAMNGVINIITRNSSETESTLVRLDAGPEEQAAALRYGGEMGQDASFRVYA
                     KWFDRGSTDRVDGTSAEDDWTKLQAGFRMDGGSGRNHFTFQGDYQTADQSFLDVPDVG
                     FTGANLLGRWEHAGDRLNTRVQVFFDRVDRERPPAGLAFDIDTYDIDFQQSASVGARH
                     HLVWGLGQRFNDYRTVNNDLAFVPNHRTLKLTNVFLQDSITLNEELKLTAGIKLEDNS
                     YSDWSTLP"
BASE COUNT          642 a         1198 c         1185 g          707 t
ORIGIN      
        1 aggtgcacga tgaactcgct gccgcgcccg aggccttcgc tgcgcgcctc gacgcgtccg
       61 ccgtgcagcg ccacgagacc tttcacgagc gcaagtccga tgccgagacc gccttcggcg
      121 cgatcgatgg cggagttgac ctgcgagaac atggtgaaga gcccgggcag ggattcctgc
      181 gtgaggccaa tgccactgtc gcggatgccg atgaccagct cgccgttctc gatacgcgac
      241 cacagcgcga tgtggccttc gggatcggtg tacttcgcgg cgttcgtcag caggttgccg
      301 atgacctgcg acaaccgcag cggatcggcc tcgagcttga tgttttccgg cgggagtttc
      361 acggtgagtt tgtgttgctt ggcatcgagc agcgggcgcg cggtttccac ggcgatgccg
      421 atgacggact tcagatcgac gtagtccttc ttgagctcga gctgtccgcg cgtgatgcgc
      481 gacacgtcga gcaggtcgtc gagcagcagc gacatgcgtt gcacctggcg cgcgatgact
      541 tcgcggcccc acttgcgctg ccggtcgtcg gcggcgtccg aatcgaggat gcgcacggcg
      601 ttgcgaatgg gcgcgagcgg gttgcgcagt tcgtgcgcga gcgtggccag gaattcgtcc
      661 ttgcgccggt cggcttccag caaggcgcgc tcggtgttct tgagacggct gatgtcgaga
      721 ttgatgccgg cccagcgctg gattttgccg tcggcgtcac ggataggcag tccttgcgcg
      781 aggatcgagt ggtatttgcc atcgacaccc atcatccggt gctcgcgata ccaggtgttg
      841 ccggtgcgcg cgcattcctt ccacgcggcc atggtttctt cgaggtcatc cggatgcatg
      901 acgctgcccc agccgtcgtt cgcggcctgc tgcatcgaga gaccgacgag gcgcaggaat
      961 gagtcgctgg catagatgca gcggccatcg gcgtcggtga cccacacgcc gtagttgatg
     1021 gactcgccga tcgcgcgata cagcttctcg ctggtctcga gcgcgcgtgc gtgcgactgc
     1081 acttcgtcga gcatcttgtt gaacgcgtcg atgacgaccc cgacttcgtc gtcggtcatc
     1141 ttctcggcgc gcacggagta gtcgcgtttc tcgatgatgg cctgcgccac cttggccatg
     1201 gattccatcg ggcgcgtgac cacgcgatgc agccagctcg acgccagcag tgcggcgatg
     1261 aggccgatga tcatgaccgc gccgagcacg ttgaggtacg cttccacgcg accctgcaca
     1321 tcgtactgcg cgctcaggta gatagtgccg agcacctctc cgccctggac gacgttcttc
     1381 atgaccagca catgaccgcc gtcgatggac aggccgctgt ttcccggcat gggcgggcgg
     1441 ttcgccgggg gcgggggttg atcggcgcgc acgtattgcg cgaacagact gccgtcttcg
     1501 gcatacagcg ccgccgcgtg aatggaatcg cgcgcctgca gtgcgctcag actgcgctgc
     1561 gcatattcgc ggtcgttgaa cgacagcgcg ggcatgacgc ccagcgcgag aatggcggct
     1621 tcggtgcgca ggtcttcagc ccacgcggcg cgattgtcgc ggaggtcggt ataaagcagc
     1681 gcagcgcccg cggacgaaag cgcaatcgcg gtggtcacca gcatgaccac catgagtttt
     1741 ccggcgacac tgcgtcgcgc gttgccgatc gagagaagcg agcgccacgg acgacgtccg
     1801 tctttgaaaa ctttactatc catcgttgat cgcatgacct tcggatcttg ccttcatata
     1861 ggaaatacgt tccactgcaa tcagccttgt cctacaagaa tagaacaaac cgttttcgcg
     1921 tcgtgttgat gatcgctgtg gcgagcttca gcgcttcgct cgtctctgcc gcacagtttt
     1981 cggtcgacgc tgtgaaggcg gcctatctgt tccggttcgc gcagtacgtc gaatggcccg
     2041 acacggcgcc gcgcgatgcg ccgttcgtcg ttgccgtcat tggtgccgaa gacgtggctg
     2101 tccacctcga gcgcttgttg ccgggcatga acgtcaatgg ccggcctacg gtcgtgcggc
     2161 gcgtgacgcg cgcccaggaa ctcgacggcg tacacatcct cttcatcggc gcggacgcgt
     2221 tcggccgcac gcgtgcgctg cgcgcccgag ccgtggagat gcccatcctg atcgtgaccg
     2281 aagaccagaa cggcatcgac ggcggcggcg tcatcaactt catcgaagtg aatcgcaatc
     2341 tgcgcttcga aatctcgctg aatgcggccg accgaagcgg ccttcggatc aactccgcgc
     2401 tgctttcggt ggcggcgcgc gtcgagcgtc gcccgcaggg cgcggtgcaa tgtctcgacc
     2461 gccatcccag ccagcagcgc tccgccgtgt gccgcatcag gatggccagc catcaatcga
     2521 ggaaaggggc atgaagatcg tgtgccggtg catcgtcatt tcattgatcc tggcggcgag
     2581 cggcatgccc gcgaacgccc aactcgtggc cgacaacagc gacccgctca ccgagctcag
     2641 ccgcatgtcg ctcgagcagc tctcgaacgt cgaagtcact tcggtctcga aatccgcgca
     2701 gacgctgtcg agcgcggcgg cgtccatcta cgtcatcacg cgcgaggaaa tcctgcgctc
     2761 gggagtgctc agcgtccccg aggcattgcg gctcgcgccc aatctccagg tgacgcagct
     2821 cacctccagc gagtactcca atggcgcccg cggttttgcc ggcgcgcccg acgtgcagaa
     2881 cttctccaac aagatcctca tcctcatcga tggccgcagc gtctactcgc cgcttttttc
     2941 cggtgtctcc tacgacatgc tcgacgtggt catggacgac atcgatcgca tcgaggtcat
     3001 ctccggtccc ggcgccacgc tctggggcgc caatgcgatg aatggcgtca tcaacatcat
     3061 cacgcgcaat tcatccgaga ccgaaagcac gctcgtgcgg ctcgacgccg gccccgaaga
     3121 gcaggccgcg gcgctgcgtt acggcggcga gatgggccag gacgcgagct ttcgcgtgta
     3181 cgccaagtgg ttcgaccgcg gctccacgga tcgggtcgac ggcaccagtg ccgaggacga
     3241 ctggaccaag ctgcaggcgg gctttcgcat ggacggcggt tccgggcgca atcacttcac
     3301 gttccagggc gactaccaga ccgccgacca gtcctttctc gatgtgcccg acgtcggctt
     3361 caccggcgcc aatttgttag gccgctggga acacgcgggc gatcgtctca acacgcgcgt
     3421 gcaggtgttc ttcgaccgcg tcgatcgcga gcggccgccg gcggggctgg ccttcgacat
     3481 cgatacctac gacatcgact ttcagcaaag cgcgagtgtc ggcgcgcgtc atcacctggt
     3541 gtggggcctg ggtcaacggt tcaacgacta ccgcacggtg aacaacgatc tcgcgttcgt
     3601 gcccaaccat cgcacgctca agctcaccaa tgtgttcctg caggactcca tcacgctcaa
     3661 cgaagagctg aaactgaccg cgggcatcaa gttggaggac aacagctatt cggactggag
     3721 cacgctgccg ga
//
LOCUS       DATDEU010000386         6865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_801621, whole genome shotgun sequence.
ACCESSION   DATDEU010000386 DATDEU010000000
VERSION     DATDEU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801621"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..791
                     /gene="ispD"
                     /locus_tag="VIV63_09165"
     CDS             75..791
                     /gene="ispD"
                     /locus_tag="VIV63_09165"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY6124809.1"
                     /translation="MRYFVVIPAAGSGRRFSASLPKQYAALGSSTVIEHALSTFESDP
                     DCGGIVVAVAADDSLWPAIAARRSRLIESVQGGAERAHSVRNALRALLARVRDDDWIM
                     VHDAARPCFSRSDLQLLKRELASHPVGGLLAVPLADTLKRALEPGAHATHVDSTIDRS
                     GLWRAATPQVFRCGVLLRALEAALDAGRTPTDEAQAIEWSGQRPLLVAGRADNIKVTT
                     ADDLALASAILGVRATQELQ"
     gene            791..1258
                     /gene="ispF"
                     /locus_tag="VIV63_09170"
     CDS             791..1258
                     /gene="ispF"
                     /locus_tag="VIV63_09170"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY6124810.1"
                     /translation="MRIGSGFDVHAFGPGDSIVLGGVRIPHSRGVVAHSDGDVVLHAL
                     CDALLGAAGLGDIGMHFPDTDPVWKGADSRRFVAAVLEMLSVRKLRISNADITLLAQT
                     PKVSPYRLEIRRSLAQMLGVTENQVNIKATTTEHLGFVGRNEGLAAQAVVLLE"
     gene            1327..2328
                     /locus_tag="VIV63_09175"
     CDS             1327..2328
                     /locus_tag="VIV63_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEY6124811.1"
                     /translation="MLRTEPEDFQVDEILGFAAGGEGPHALLQVRKRGANTEWVAREL
                     ARAAGVKPFDVGFAGLKDRHAVTTQHFTVPRGKRPAEEFIGMAGEGFEVLSAAAHQRK
                     LPRGALEGNRFAIVVRGIACDPAALAQRMHHIATGGTPNYFGEQRFGREAGNLEQVLR
                     AAQQVAEGQGRRRERRGRDDAGFMLSAARSLIFNALLAERVRQGTWNRLAVGDVANLD
                     GRGSVFAVEQVDDELEQRCAVLDVHPTAPLAGAGESLAKGDVLVLEDTVAARYPEALS
                     VIHAEGMKSERRALRIRVRGLEHEYSGDTLHLRFALSAGSFATTVLREIIAGAATGE"
     gene            2331..3086
                     /locus_tag="VIV63_09180"
     CDS             2331..3086
                     /locus_tag="VIV63_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY6124812.1"
                     /translation="MLDISDEGSVRIIKLARPPVNALNGELVRNLIAALDGPADTAAI
                     VIAGQPGLFSAGLDVPGLLALDREGLAGMFVELWRAQRAIATSPVPIVFAINGHCPAG
                     GTVLAINGDYRVMAQGEFRLGLNEVQVGLFPGGVIHGAFKRLVAGHTAQLLTRGALID
                     PATALRIGLVDELCDPAQVPARALEVANELCALPREPMLRTRAMARRDLTDLYGPAEE
                     AASCEREFGEMGVQMWFAPATQELLRRQFTKKR"
     gene            complement(3195..3728)
                     /locus_tag="VIV63_09185"
     CDS             complement(3195..3728)
                     /locus_tag="VIV63_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY6124813.1"
                     /translation="MSGKRTDPDTDVFREAMRDVKPIAARPVVPPGKRKPPAKARFTA
                     ADRAMVLVESLQGIPAGESTDSGDEISFRRIGVQDSVLRKLKRGEYRVEEICDLHGLR
                     VDEAKAALREFLAEALAHHLRCVRIIHGKGMNSGPRGPVIKTAVNMILRKTAPVLAFT
                     SARRVDGGTGAINVLLR"
     gene            3768..4541
                     /gene="surE"
                     /locus_tag="VIV63_09190"
     CDS             3768..4541
                     /gene="surE"
                     /locus_tag="VIV63_09190"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY6124814.1"
                     /translation="MRILISNDDGYRAPGIKALREGLEGLGDLTVVAPDRNRSGASNS
                     LTLELPLRVGQAEPDVYFVEGTPTDCVHLAVSGLFPFEPDMVVSGVNDGSNLGDDVLY
                     SGTVAAATEGRFLGKAAVAVSLHTDGLRGDATRHFATGAHFARVIVERLSATPLTLGG
                     RATILNVNVPNIPVSAVKGIQVTRLGNRHRAEGVIKAEDPRGRPVYWVGPAGASQDAG
                     PGTDFHAVAEGYVSVTPLTVDLTNHAALEGLRSWFGPGA"
     gene            4568..5203
                     /locus_tag="VIV63_09195"
     CDS             4568..5203
                     /locus_tag="VIV63_09195"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY6124815.1"
                     /translation="MTSARTRDRLVQRLREQGITNPAVLDRIRNVPRHIFVDEALASR
                     AYEDTALPIGFRQTISQPYIVARMTEALLEGGRKIGKVLEVGTGCGYQTAVLAPFVKK
                     IYSIERIEPLAKRAKATLKELDIKNVMLKHGDGTTGWKSQKPFDGILVAAAPLAVPEA
                     LFAQLANKGRLIVPVGPEGAQQLIRFTRRGDELDREVLGAVAFVPMLGGTA"
     gene            complement(5549..5938)
                     /locus_tag="VIV63_09200"
     CDS             complement(5549..5938)
                     /locus_tag="VIV63_09200"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEY6124816.1"
                     /translation="MKITQHREDGINLIRRYGADFVAIGDREIRESCIVAANSLTPWA
                     PRFVEELDAGHFAPLFALEPEVVVLATGARQSFPRAALRAEFATRKIGLEVMEIGAAC
                     RTYNVLVGELRKVVAAILLPGPSKGPE"
     gene            5959..>6865
                     /locus_tag="VIV63_09205"
     CDS             5959..>6865
                     /locus_tag="VIV63_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124817.1"
                     /translation="MRRLTLILSDLYLPEETERGAAVPAARDLPNLEWLMRFAGSPHR
                     IGDWRAWLMAQTAPGFGSFPLAVNCAYGRIDDGLLDSTWLATPVALEARLDHVRLLDR
                     GLLRLEEAERASCREEFARVFGPKVLLHEGGERGFFLSGLPAAGVNTVDPARLLGTEI
                     GPAMPGREAGELRRLWTEIEMWLHGAAFNTQRERARKPRVSALWLWGARSTPETRGRV
                     EAWRASAAYFGGDPLIAALARCDSHRARGVPKQWAHLDAAEPHVVVEFAALTGGPHES
                     LEALDANWFAPAKAALATGDLAELDL"
BASE COUNT         1155 a         2241 c         2276 g         1193 t
ORIGIN      
        1 ccgcgcaccg ccctgcccac gcggccggcc gaacacacca agaccgccgc gcgctgacgt
       61 aagctacgcg ccgcatgcgc tatttcgtcg tcatcccggc cgcgggcagt ggccgtcgat
      121 tctccgcgtc ccttccgaag caatacgccg ccctcggtag ttcgacggtc atcgaacacg
      181 ccttgagtac cttcgagtcc gatccggact gcggaggcat cgtcgtcgca gtcgccgccg
      241 acgattcgct gtggcccgcc atcgcggcgc gccgctcgcg tctcatcgaa tccgtgcagg
      301 gtggcgcgga gcgtgcgcac tccgtgcgca acgcgctgcg cgccttgctg gcgcgcgtgc
      361 gcgacgacga ctggatcatg gttcacgacg cggcgcggcc gtgtttctcc agatcggatc
      421 tccaattgct caagcgtgag ctcgcgtcgc atccggtggg aggtctgctg gcggtgccgc
      481 tggccgacac attgaagcgc gcgctcgagc ccggcgcgca tgccacgcac gtcgactcga
      541 cgatcgatcg cagcggactg tggcgcgccg ccacgcccca ggtatttcgt tgcggggtgc
      601 tgctgcgggc gctcgaggcc gcgctcgacg caggccgcac gcccaccgac gaagcccagg
      661 ccatcgaatg gagtggacag cgacccctgc tggtcgccgg ccgcgccgac aacatcaagg
      721 tcaccaccgc cgacgatctc gcgctcgcct cggccatact cggcgtgcgc gcgacacagg
      781 agctccagta atgcgaatcg gttccggttt cgacgtgcat gccttcggtc cgggcgactc
      841 catcgtgctc ggcggcgtgc gcatcccgca ttcgcgcggc gtggtggcgc acagcgacgg
      901 ggacgtcgtg ctgcatgcgc tctgcgatgc gttgttaggc gccgcgggcc tcggtgacat
      961 cggcatgcac tttcccgata cggatccggt gtggaagggc gccgacagcc ggcgctttgt
     1021 cgccgcggtg ctcgagatgc tcagcgtgcg caagctcagg atcagcaatg cagacatcac
     1081 gctgctggcg cagacgccca aggtgtcgcc ctatcgtctc gagatccgcc gctcgctcgc
     1141 gcagatgctc ggggtcacgg aaaaccaggt gaacatcaag gccacgacca ccgagcacct
     1201 gggtttcgtg ggtcgcaacg agggtctcgc ggcgcaggca gtcgtgttgc tcgaatgatc
     1261 cccgaacact ggcgccatgc agcattggcg ccgcctcctg cctggggcgc gccactgggt
     1321 accggagtgc tgcgcacgga acccgaagac ttccaggtgg acgaaatcct gggattcgcg
     1381 gcgggcggcg agggtccgca cgcactgctg caggtgcgca agcgcggcgc caataccgag
     1441 tgggtggcgc gggagctggc gcgcgccgcg ggcgtgaagc ctttcgacgt gggtttcgcg
     1501 gggctcaagg atcgccatgc cgtcaccaca cagcacttca ccgtgccgcg cggcaagcgc
     1561 ccggcggaag agttcatcgg aatggcgggc gagggtttcg aggtgttgtc cgcggccgcg
     1621 caccagcgca agttgccgcg cggcgcgctc gagggaaacc gcttcgcgat cgtcgtccgt
     1681 ggtatcgcct gcgatccggc cgcgctcgcg cagcgcatgc accacatcgc caccggcggc
     1741 acgcctaact atttcggcga gcagcggttt ggtcgcgagg cgggcaatct cgagcaggtg
     1801 ctgcgcgccg cgcaacaggt ggccgaaggg cagggccgcc ggcgcgaacg gcgcgggcgc
     1861 gacgacgcag gcttcatgct gtcggccgcg cgcagtctca ttttcaacgc cctgctcgcc
     1921 gaacgagtga ggcagggcac ctggaaccgg ctcgcggtgg gtgacgtcgc gaacctcgat
     1981 ggtcgcggca gcgtattcgc agtggaacag gtcgacgacg agctcgagca acgttgcgcc
     2041 gtgctcgatg tgcatcccac ggcgccgctg gcgggcgccg gcgaatccct ggccaaaggc
     2101 gacgtgctcg tgctcgagga cacggtcgcg gcgcggtacc cggaagcact gtccgtgata
     2161 cacgcagaag gcatgaagtc cgaaagacgc gcgttgcgca tccgggttcg cggtctcgaa
     2221 catgaatatt ccggcgacac gctgcacctg cgattcgccc tgtccgccgg aagttttgcg
     2281 accaccgtgc ttcgcgagat catcgcgggc gcggctacgg gagagtgagc atgctcgaca
     2341 tcagtgacga aggcagcgtt cggatcatca agctcgcacg tccacccgtg aacgcactca
     2401 acggcgaact ggtgcgaaat ctcatcgccg cgctcgacgg acctgccgat acggcggcga
     2461 tcgtcatcgc gggtcagccc ggcctgtttt cggctggcct cgacgttccg ggtcttctcg
     2521 cgctcgaccg cgaaggactc gcgggtatgt tcgtcgagct gtggcgcgct caacgcgcca
     2581 tcgccacctc accggtgccc atcgtgttcg ccatcaacgg ccactgtccg gcgggcggca
     2641 ccgtgctcgc gatcaacggc gattaccgcg tcatggcgca gggcgagttc cggctcggac
     2701 tcaacgaagt gcaggtcggt ctttttccgg gtggcgtgat ccacggcgcc ttcaagcgcc
     2761 tggtggccgg tcacaccgca cagctcctga cgcgcggtgc gctcatcgat cccgcgacgg
     2821 cgcttcgcat cggcctggtc gacgagctgt gcgacccggc gcaggttccg gctcgcgcat
     2881 tggaagtggc aaacgagttg tgcgcgttac cgcgcgagcc catgttgcgc acgcgcgcga
     2941 tggcgcgtcg cgatctcacc gacctgtacg gcccggcgga agaagcggcg agctgtgagc
     3001 gcgaattcgg cgagatgggc gtgcagatgt ggttcgcgcc tgccacgcaa gaactgctca
     3061 ggcggcaatt cacgaagaag cgttaagaga aaaggggaca gatctatttt agaaaagggg
     3121 acagatctat ttttcgcctc aaaaaataga tctgtcccct tttctaaaat agatctgtcc
     3181 ccttttctcc ccttttagcg tagcagcacg ttgatcgcgc ccgtgccgcc gtcgacgcgg
     3241 cgcgccgagg tgaacgcgag taccggggcg gtcttgcgca ggatcatgtt cacggcggtc
     3301 ttgatgacgg gcccgcgcgg ccccgagttc atgcccttgc cgtggatgat gcgcacgcag
     3361 cgcaggtggt gcgcgagcgc ctcggccagg aattcccgca gcgccgcctt ggcttcatcg
     3421 acgcgcaatc cgtgcaggtc gcagatctcc tcgacacggt attcgccgcg cttgagtttg
     3481 cgcaggacgg agtcctgcac gccgatgcgg cgaaaggaga tctcgtcgcc agagtcggtg
     3541 ctctcgccag ctggaatgcc ctgcaggctt tccaccagca ccatggcgcg atccgcggcg
     3601 gtgaatcgcg ctttcgccgg cggcttgcgt tttcccgggg gcacgaccgg gcgtgcagcg
     3661 atcggcttga cgtcccgcat cgcctcgcga aacacatccg tgtccggatc ggtgcgtttg
     3721 ccgctcaagc tacctccgcg ttgatgcatt gagtatattg cgcgccggtg cgaatcctca
     3781 tatccaacga cgacggttac cgggctccgg gcatcaaggc gctgcgcgaa gggctcgagg
     3841 gtctgggcga tctgactgtc gtcgcgcccg accgcaaccg cagcggcgcc agcaattcgc
     3901 tcacgctcga acttcctttg cgggtcggcc aggcggagcc cgacgtctac tttgtagaag
     3961 gcacgccgac tgattgcgtt catctcgcgg tatcggggct gttcccgttc gaacccgaca
     4021 tggtggtttc cggagtcaat gacggctcga atctcggcga cgacgtgttg tattcgggaa
     4081 ccgttgcggc cgccaccgag ggccgtttcc tgggcaaagc cgccgtggcg gtctctctgc
     4141 acaccgacgg cttgcgcggt gacgccacgc gccacttcgc gaccggtgca catttcgcgc
     4201 gcgtcatcgt cgaacgtctc agcgcgacgc cactcacgct cggcggccgc gccaccattc
     4261 tcaacgtcaa cgtgcccaac attccggtga gcgcggtgaa aggcatccag gtcactcgtc
     4321 tcggcaatcg tcatcgcgcc gaaggggtga tcaaggcgga agatccgcgc ggacgtccgg
     4381 tctactgggt gggacccgcg ggcgccagcc aggatgcggg acccggaacc gatttccacg
     4441 ccgtggccga aggctacgtg tccgtcacgc ccctcacggt ggatctcacc aaccacgcgg
     4501 cgctcgaagg attgcgcagc tggttcgggc ccggcgcgtg agcgaacacc ggaccgccgg
     4561 catcggcatg acctccgcgc gcacgcgcga ccggctggtc cagcggctgc gcgagcaggg
     4621 catcaccaac cccgcggtgc tcgaccgcat ccgcaacgtt ccgcggcaca tcttcgtcga
     4681 cgaagcgctg gcgtcgcgcg cctacgaaga caccgcgttg cccatcggat ttcgccagac
     4741 catttcgcag ccctacatcg tcgcgcgcat gaccgaagcg ctgctcgaag ggggccgcaa
     4801 gatcggcaaa gtgctcgaag tgggcacggg ctgcggttat cagaccgcgg tgctcgcgcc
     4861 gttcgtgaag aagatctact cgatcgagcg catcgagccg ctcgcgaaac gcgcgaaggc
     4921 gacgctcaag gaactcgata tcaagaacgt gatgctcaag catggcgacg gcaccaccgg
     4981 ctggaaatcg cagaagcctt tcgacggcat cctggtggcc gcggcaccgc tggcagtgcc
     5041 cgaagctctg ttcgcgcagc tcgccaacaa agggcgtctc atcgttcccg tggggcccga
     5101 aggtgcgcag caactcattc gctttacgcg gcgcggcgat gagctcgatc gggaggtgtt
     5161 aggggcggtt gcgttcgttc cgatgctcgg cggcaccgcc tgaccattgc gcgctgcgtc
     5221 agggtattcg agtaaattct cggatacggt atgttctgta actctttgtt tttgcagctt
     5281 cgataccacc tggatttgca tgggattgtc agcccactaa ggcaaaggct tgtcagtcag
     5341 gatttaacat gttttgatgt cgccgcccgc cgacagagac cctagtaaga gtttgcgagg
     5401 ggtcccggat gaaggcacag gggagaacgg ggaagtcgcg gcaataagaa gaacaagccg
     5461 aatagaagaa aaaaaaattc gccagtaatt cgagtctgtc agcttcgaac cattggcctc
     5521 gctaaacaaa ctttctcccc ctgtgtcttc attcgggtcc cttcgaggga cccggcagca
     5581 gaatcgccgc caccactttg cgaagttcgc cgaccagcac gttgtaggtg cggcaggccg
     5641 cgccgatctc catcacctcg agtcctatct tccgcgtcgc gaactccgcg cgtagcgccg
     5701 cgcgcggaaa cgattgacgc gcaccggtcg ccagcaccac cacttcgggt tcgagggcga
     5761 acaagggcgc gaaatgtccg gcgtcgagtt cttcgacgaa tcgcggcgcc cacggcgtga
     5821 gcgagttcgc ggcgacgatg cagctttcgc ggatctcgcg atcgccgatc gcgacgaagt
     5881 ccgcgccgta acggcggatg aggttgatac cgtcctcgcg gtgctgagtg atcttcatgg
     5941 gtccgatacg ataccgccat gcgccgtctc acgctgattc tctctgacct ctacctgccg
     6001 gaggagacgg agcgtggggc cgccgttccg gcggcgcgcg acctgcccaa tctcgaatgg
     6061 ctgatgcgct tcgcgggttc accgcaccgg atcggtgact ggcgcgcatg gctcatggcg
     6121 caaacggcgc caggcttcgg atctttcccg ctcgcggtca actgcgcgta tggacgaatc
     6181 gatgacggcc ttctggactc gacctggctc gcgacgcccg tcgcgctgga agcgcggctc
     6241 gaccacgtga ggctcctcga tcgcgggctg ctgcgcctcg aagaagcgga gcgggcgagc
     6301 tgtcgcgaag aattcgcgcg tgtgttcgga ccgaaggtcc tgctccacga gggcggcgag
     6361 cgtgggttct tcctcagcgg attgccagcg gctggcgtga ataccgttga tccggcgcgg
     6421 ctgctcggca cggagatcgg tccggcgatg cccggtcggg aagccggcga gttgcgacgc
     6481 ctctggacgg aaatcgaaat gtggctgcat ggcgcggcgt tcaacacgca gcgcgagcgc
     6541 gcgcgaaaac cgcgtgtgtc cgctctgtgg ttgtggggcg caaggtcgac acccgaaacc
     6601 cggggtcgcg tggaagcctg gcgcgcaagc gcggcgtact tcggcggcga tccgctgatc
     6661 gccgcccttg cacgatgcga ctcgcaccgc gcacgcggcg ttccaaaaca gtgggcgcac
     6721 ctcgatgccg ccgaaccgca cgtggtggtg gagttcgcgg cactgacagg tggaccgcac
     6781 gagtcgctgg aggcgctgga cgccaactgg tttgcgccgg cgaaggcagc gctcgcgacc
     6841 ggcgacctcg cggaactcga cctgg
//
LOCUS       DATDEU010000387         4646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1530824, whole genome shotgun sequence.
ACCESSION   DATDEU010000387 DATDEU010000000
VERSION     DATDEU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530824"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VIV63_09210"
     CDS             complement(<1..527)
                     /locus_tag="VIV63_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124818.1"
                     /translation="MSKKFLVAILAAMGCAAHAASKPEPVNAQLVIRADTPGSTIDAN
                     IYGQFMEHLGRNVYEGIWVGEDSTIPNTRGFRNDTLAALRKLKVPVLRWPGGCFADDY
                     HWRDGIGYRAKRPARVNTFWGGVIEPNTFGTHEFFELAELLGAKTYLAVNVGSGTVAE
                     MSQWVEYITSPSQSAL"
     gene            complement(627..1310)
                     /locus_tag="VIV63_09215"
     CDS             complement(627..1310)
                     /locus_tag="VIV63_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6124819.1"
                     /translation="MLQLFIGNKNYSSWSLRPWVLLRELNIPFAERLVPFAGAQNPEA
                     FRGFSPTGKVPCLVDGEVTVWDSLAITEYVAERFTGVWPIEGVTRAWARSATAEMHSG
                     FFGLRNACTMNCGLRIALNSIEPALARDLARLEELWADGIGRFGGPFLTGARFCAVDA
                     FFVPVAFRIQTYDPPVGAAARRYAATLLALPSAKEWYEAALQETWRDEEHEVEARAAG
                     AWRADLRAH"
     gene            complement(1315..1803)
                     /locus_tag="VIV63_09220"
     CDS             complement(1315..1803)
                     /locus_tag="VIV63_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6124820.1"
                     /translation="MQISPDPSAGTDAVEIRPAAPTDLEVLLPMVEQYWRFEAIEGFD
                     AARMRKLLTRVLEDANLGRAWVASLYGEPAGYLLAVYVFSLEHQGLTAEIDEFFIVPA
                     HRGLGLGHQLLSIAEAQFRLEGCTNVSLQLGRSNEAARNFYRRHGFDDRAGYELVSKN
                     LK"
     gene            complement(1857..3155)
                     /locus_tag="VIV63_09225"
     CDS             complement(1857..3155)
                     /locus_tag="VIV63_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124821.1"
                     /translation="MAGWATLWLGGIALLSLITLATARYFIESPWMAAITAALVLTPV
                     IAWSGTLLAARWSRMARALTDGIASLKDRDFSVSVTRATQDEMGDLVTTYNSLGDRLR
                     VERQSLYQRELMLDTVIQTTPLALVLTNEGDAVLYSNTAARQLFSAGRKLEGERFTRF
                     LDAAPVPLREAIERGGDTLFTLELDGEPQVYHVSQRRFLLNALPHRLLLLKQLTREIN
                     SQEVATWKKVIRVIAHELNNSLAPISSLAHSGQILAHAPPDPEQMIRVFSTIEERARH
                     LAGFIEGYAQFAKLPQPRVAPVAWNILLERLRVVVQFTLAGAPPQRVISFDAAQIEQA
                     LINLLKNARESGSEAADIELAVSPAGGGCAVEVRDRGPGFSPAALEHALVPFYSTKQT
                     GTGLGLTLCREIVEAHGGRLRIANREGGGAVVTLWLPDTA"
     gene            complement(3219..4598)
                     /locus_tag="VIV63_09230"
     CDS             complement(3219..4598)
                     /locus_tag="VIV63_09230"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6124822.1"
                     /translation="MTSVTSNVEDRLANRTVLVADDSEAVRTAFQVLLSLHGARVLGA
                     SSPAEALATVRSQEVDLVIQDMNFRREATSGEEGIALFRELRALHPDVPVILLTAWTH
                     LETAVDLVKAGAADYIAKPWDDARLLTTVRNLLELRAAVEENVTTRARRAEARATLAE
                     RYDLQGLVYESEALHTVASMAAQVAHADVPVLITGPNGAGKEVLADIVHANSARKSQP
                     YIKVNAGALPDQLIEAELFGTEAGAFTGAKARAGRFEAADGGTLFLDEIGNLPLAGQA
                     KLLRVLQTGEYERLGSNTTRRANVRIVAATNMPLRDAMRAGTFREDLYYRLSVIELAL
                     PSLADRRDDIMPLARAFLAKGARLTGDAERALVNHSWPGNVRELRNVLQRAALLAGEA
                     PINAAALNLPAVLPARSDDPVLDRGTIERVLAENDNIVAQAARQLGISRQALYRRMEK
                     LGIKESPSR"
BASE COUNT          774 a         1454 c         1630 g          788 t
ORIGIN      
        1 agcgccgatt gtgagggcga ggtgatgtat tcgacccact gcgacatctc ggcgacggtg
       61 cccgagccga cgttcaccgc gagataggtc ttggcgccca ggagttccgc gagctcgaag
      121 aactcatgcg tgccgaaggt attgggttcg atgacgccgc cccagaacgt gttgacgcgg
      181 gcggggcgct tggcgcgata gccgatgcca tcgcgccagt gataatcgtc ggcgaagcat
      241 ccaccgggcc agcgcagcac cggcaccttg agtttccgga gcgccgcgag cgtgtcattg
      301 cggaagccgc gcgtgttggg gatggtggaa tcttcgccga cccagatgcc ttcgtagaca
      361 ttgcggccca ggtgttccat gaactgcccg tagatgttgg cgtcgatcgt ggagccgggc
      421 gtgtcggcgc gaatgaccaa ctgcgcgttc accggttccg gcttcgacgc cgcgtgagca
      481 gcacaaccca tggcggccag gatggccacg agaaatttct tcgacatgtg atccccttcg
      541 tgccggcaac gatgcggcaa ggcgtcggag actagcgtat ccgcgcgttc acgacagccc
      601 gtgagcgatc acaaaaaacg tatggttcag tgggcgcgca ggtcggcgcg ccaggcaccg
      661 gcggcacggg cttcgacttc gtgttcttcg tcacgccagg tttcctgcag cgcggcttcg
      721 taccattctt tcgcggacgg caaagccagc agggtcgcgg cgtagcgtcg cgcggcggcg
      781 cccaccggcg gatcgtaggt ctggatgcgg aatgcaaccg gaacgaagaa cgcatcgacg
      841 gcgcagaagc gcgcaccggt gaggaacggc ccgccgaaac ggccgatgcc atcggcccag
      901 agttcctcga gccgcgcgag gtcgcgcgcg agcgcaggtt cgatggagtt gagcgcgatg
      961 cgcagcccgc agttcatcgt gcaggcattg cgcaatccaa agaaccccga gtgcatctcg
     1021 gcggtggcgg atctcgccca ggcgcgtgtc acgccttcga tcggccacac gccggtgaaa
     1081 cgctcggcca cgtattcagt gatggccagc gaatcccaca ccgtgacctc gccgtcgacg
     1141 agacagggca ccttgccggt gggcgaaaag ccgcgaaatg cttcggggtt ctgcgcgccg
     1201 gcgaaaggga ccagccgttc ggcgaatgga atgttcagct cgcgcaacaa cacccagggg
     1261 cgcagcgacc acgacgaata gttcttgttg ccgatgaaca gctggagcat gcgcctactt
     1321 caggttcttg ctcaccagct catacccggc gcgatcgtcg aatccgtggc gccgatagaa
     1381 attgcgcgcg gcttcgttgc tgcggccgag ctgcaacgag acgttggtgc agccctcgag
     1441 acgaaactgc gcttcggcga tcgacagcag ctggtggccg agtcccaggc cgcgatgcgc
     1501 gggcactatg aagaattcgt cgatctcggc ggtgaggccc tggtgttcga ggctgaacac
     1561 atagaccgcc agcaggtagc ccgcgggctc gccgtataac gatgccaccc aggcgcgtcc
     1621 gaggttggcg tcttccagca cgcgcgtgag cagcttgcgc atgcgggcgg cgtcgaagcc
     1681 ttcgatggcc tcgaagcgcc agtactgttc gaccatcggc agcagcactt cgaggtcggt
     1741 gggcgccgcg gggcggatct cgacagcgtc ggtgccggca gaaggatcgg gggagatttg
     1801 catcgcggcg attctctcat cgcgacgcgg gtgcgggcca gccgggatag gtcgtcctac
     1861 gcggtgtccg gcagccacaa cgtcacgacc gcgccgccgc cctcgcggtt ggcgatgcgc
     1921 aaccggccgc catgcgcttc gacgatttca cggcacaacg tcagcccgag gcccgtgccg
     1981 gtttgtttgg tcgaatagaa cggcaccagc gcatgttcca gcgcagctgg cgaaaagccg
     2041 ggcccgcgat cgcgcacctc gaccgcacag ccgccgccgg cgggcgagac cgcgagctcg
     2101 atgtcggccg cttcggagcc ggactcgcgg gcgttcttca agaggttgat caacgcctgc
     2161 tcgatctggg cggcgtcgaa actgatgacg cgctgcggcg gcgcgcccgc gagcgtgaat
     2221 tgcacgacca cgcgcaatcg ttccagcagg atgttccagg ctacgggcgc gacgcgtggc
     2281 tgcggcagct tcgcgaactg tgcgtagcct tcgatgaaac cggcgagatg acgcgcgcgc
     2341 tcttcgatgg tggagaacac gcggatcatc tgttcgggat ccggcggcgc atgcgcgagg
     2401 atctggccgg aatgggcgag cgaggaaatc ggcgcgagcg agttgttcaa ttcgtgcgcg
     2461 atgacgcgaa tcaccttctt ccaggtcgcc acctcctggg aattgatttc ccgcgtgagc
     2521 tgcttgagca gcaacagccg atgtggcagc gcgttcagca ggaagcgccg ctgcgacacg
     2581 tgatagacct gcggctcgcc gtcgagctcc agcgtgaaca acgtgtcgcc gccccgctcg
     2641 atggcttcgc gcaggggcac cggcgccgcg tcgaggaatc gcgtgaagcg ttcaccctcg
     2701 agcttgcggc cggcgctgaa gagctggcgc gccgcggtgt tgctgtagag cacggcgtcg
     2761 ccttcgttgg tgagcaccag cgcgagcggc gtggtctgga tgacggtgtc gagcatgagc
     2821 tcgcgctggt agaggctctg gcgctcgacg cgcaggcgat cgcccaggct gttgtacgtg
     2881 gtcaccaggt cgcccatctc atcctgcgtg gcgcgggtga cgctcacgct gaagtcacga
     2941 tccttgaggc tggcgatgcc gtcggtgagc gcgcgcgcca ttcggctcca gcgcgcggcc
     3001 agcagggtgc cggaccaggc gatgaccggc gtgagcacga gcgcggcggt gatcgcggcc
     3061 atccacggcg attcgatgaa gtagcgcgcc gtggcgagtg tgatgagcga cagcagcgcg
     3121 atgccgccga gccacaacgt ggcccagccg gccatggacc ctctgaatgc agagcgcatc
     3181 aatcccggaa ttcccgcctc ttgttagtcc cggcgtggct accgggaagg agattccttg
     3241 ataccgagtt tttccatgcg gcgatagagg gcctgccggc tgatgccgag ctggcgcgcc
     3301 gcctgcgcca cgatattgtc gttctcggcg agaacgcgtt cgatggtgcc gcgatcgagc
     3361 acggggtcgt cggagcgcgc gggtaaaacg gccgggaggt tgagcgctgc ggcgttgatg
     3421 ggcgcttcgc ccgcgagcaa tgctgcgcgt tgcagcacat ttctgagttc gcggacgtta
     3481 cccggccagg aatggttgac gagggcgcgt tcggcgtccc ccgtgagtcg cgctcccttc
     3541 gccaggaacg cgcgtgccag cggcatgatg tcgtcacgcc ggtcggcgag cgagggcaac
     3601 gcgagttcga tgacagagag gcggtaatag agatcttcgc ggaaggtgcc cgcgcgcatg
     3661 gcgtctcgca gcggcatgtt ggtggcggcg acgatgcgca cgttggcgcg gcgcgtggtg
     3721 ttcgacccca ggcgctcgta ctcgccggtc tgcaacacgc gcagtagttt ggcctggccg
     3781 gccagcggca ggttgccgat ctcgtcgagg aacaacgtgc cgccgtcggc ggcttcgaat
     3841 cgtccggcgc gcgccttggc gccggtgaac gcgccggctt cggtgccaaa caactccgct
     3901 tcgatgagct gatcgggcag ggcgcccgca ttcaccttga tgtagggctg cgacttgcgc
     3961 gcggagttgg catgcacgat gtcggccagc acttccttgc cggcgccgtt cgggccggtg
     4021 atgagtacgg gaacgtccgc gtgcgccacc tgcgcggcca tcgacgccac cgtgtgcagc
     4081 gcctcgcttt cgtagacgag cccctggagg tcgtagcgct cggccagcgt ggcgcgtgct
     4141 tcggcccgcc gtgcgcgcgt ggtgacgttc tcctcgacgg ctgcgcgcag ctcgagcagg
     4201 ttgcgcaccg tggtcagcag gcgcgcgtcg tcccagggct tggcgatgta atccgcggcg
     4261 ccggccttca cgaggtccac ggcggtttcc aggtgcgtcc aggcggtcag caggatcacc
     4321 ggcacgtcgg gatgcagcgc gcgcagctcc ctgaacagcg cgatgccctc ttcgccggag
     4381 gtagcctcgc gccggaagtt catgtcctgg atgaccaggt ccacttcctg ggagcgcacg
     4441 gtcgcgaggg cctcggccgg agatgaggcg ccgaggaccc gcgcgccgtg cagggacaac
     4501 aggacctgga aggccgtgcg cacggcctcg ctatcgtcgg cgacaagtac agtccggttg
     4561 gccaggcgat cctcgacgtt ggatgtcact gaggtcatgc gtaagacgct cgggatcggt
     4621 ttagggttgc aaacggcgga cacttt
//
LOCUS       DATDEU010000388         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_510644, whole genome shotgun sequence.
ACCESSION   DATDEU010000388 DATDEU010000000
VERSION     DATDEU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510644"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VIV63_09235"
     CDS             <1..709
                     /locus_tag="VIV63_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6124823.1"
                     /translation="RVVAAVKDYREKHPTPDVRYRLATGNVGVMAATNEEVKAKEYPI
                     LGWVFAAVILMCLLTFRSLLGTVLVFLPLALVSILVYAVMAMVGIGLKVNTLPMVALG
                     AGIGVDYGIYLWSRMQEYLRAGDTVHQAFEKTMRVTGASIIFTGITLAIGVVTWVFSP
                     LQFQADIGIMLTFMFFVNMLGAIILLPALAAWLVKPKSLADRRDEGGDTNADAQNGDT
                     RRGDSEAHDGPSMDADD"
     gene            709..>2044
                     /locus_tag="VIV63_09240"
     CDS             709..>2044
                     /locus_tag="VIV63_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY6124824.1"
                     /translation="MTPEEFRRVGHELIDWVADYRERVARMEFPVMARTTAGALRAAL
                     PPSPPQEPDAFAAIRKDLDELILPACTHWQDPRFHGYFPSNGLPAALLGDLVSTGLGQ
                     LGLNWQSSPALTELEEVSLDWWRQMLGLSPAWSGVIQDTASTSTFVALVCARERCTGF
                     SATRAGMQAERMPLVVYTSSQAHSSVEKAALLAGFGRQNVCAVPVDENFDLKPEALAA
                     AIAADRAAGKHPCAVVATCGSTATTSFDPITPLAVIAAREDIWLHVDAAMAGSAMILP
                     ECRALWDGIEAADSLVVNPHKWLGVSFDCSTYYVRDPQFLVRVMSTNPSYLQTAADGL
                     VRNYRDWGVPLGRRLRAMKLWFVIREQGVSGLQARLRRDLQNAAWLAAEVSRAPHWKI
                     LAPVRLQTLVLRHEPPGLDADAIDAHTRAWAAAVNESGEAYLTPAVIDGRWAV"
BASE COUNT          320 a          679 c          695 g          350 t
ORIGIN      
        1 gcgcgtcgtc gcggccgtga aggactaccg cgagaagcac ccgacgccgg acgtcaggta
       61 ccggctggcg accggcaacg tcggcgtgat ggcggccacc aacgaggaag tgaaggccaa
      121 ggaatatccc atcctcggct gggtgttcgc cgcggtcatc ctcatgtgcc tgctgacgtt
      181 ccgcagcctg ttgggcacgg tgctggtgtt cctgccgctc gcgctcgtgt ccatcctggt
      241 ctacgcggtc atggccatgg tcggcatcgg cctcaaggtg aacacgctgc ccatggtggc
      301 gctcggcgcg ggcatcggcg tggactacgg catctacctg tggagccgca tgcaggagta
      361 cctgcgcgcc ggcgacaccg tgcaccaggc gttcgagaag accatgcgtg tgaccggcgc
      421 cagcatcatc ttcaccggca tcacgctcgc catcggcgtg gtcacctggg tgttctcgcc
      481 gctccagttc caggcggaca tcggcatcat gctcacgttc atgttcttcg tgaacatgct
      541 gggcgcgatc atcctgctgc cggcgctcgc ggcgtggctg gtcaagccga agagtctggc
      601 ggaccggcgc gacgaaggcg gggatacgaa cgccgacgcg caaaacggtg atacgcgccg
      661 cggcgactct gaagctcacg atggcccgtc catggacgcg gacgactgat gacgccggag
      721 gagttccgcc gcgtcggtca cgaactcatc gactgggtcg cggactatcg cgagcgcgtc
      781 gctcgcatgg aattccccgt gatggcgcgc actaccgcgg gcgccttgcg cgcggcgttg
      841 ccgccatcgc caccgcagga gcccgacgcg ttcgctgcga tccgcaagga tctcgacgaa
      901 ctgatccttc ccgcctgcac gcattggcag gatccgcgct tccatggcta tttcccctcc
      961 aacggtctgc ccgcggccct gctgggcgac ctcgtgagca cgggcctcgg acaattgggc
     1021 ctcaactggc agtcgagtcc ggcgctcact gaactggaag aggtgagtct cgactggtgg
     1081 cggcagatgc tgggcctgtc gccggcctgg tccggggtca tccaggacac ggcatccacc
     1141 tcgacgttcg tggcgctggt gtgcgcacgc gagcgctgca cgggcttcag cgccacgcgc
     1201 gccggcatgc aggccgagcg catgccgctc gtggtgtaca cgtcctcgca ggcgcacagc
     1261 tcggtggaga aggccgcgtt gctcgcgggc ttcggccgcc agaatgtctg cgccgtgccg
     1321 gtggatgaga acttcgacct gaagcccgag gcactggcag ccgcgattgc ggccgatcgc
     1381 gcggccggta aacatccctg cgccgtggtc gccacctgcg gaagcaccgc gacgaccagc
     1441 ttcgatccga taacaccgct tgcggtgatc gccgcacgcg aagacatttg gctgcacgtc
     1501 gacgccgcca tggcgggctc ggcgatgatc ctgccggaat gccgcgcgtt gtgggacggc
     1561 atcgaggccg ccgactcgct ggttgtcaat ccacacaagt ggttaggcgt gtctttcgac
     1621 tgttccacct actatgtgcg cgatccgcaa ttcctggtgc gagtgatgtc gaccaatccg
     1681 agctacctgc agaccgcggc cgatggcttg gtcaggaact accgcgactg gggcgtgccg
     1741 ctcggccgcc gcctgcgcgc gatgaaactg tggttcgtga tccgcgagca gggcgtaagc
     1801 gggctgcagg cacggctgcg gcgcgatctg cagaacgccg cgtggctggc cgccgaagtt
     1861 tcgcgggcgc cgcactggaa gatcctggcg cccgtgcggc tgcagacact ggtgttacgc
     1921 catgaaccgc caggtctcga cgccgacgcc atcgatgcac atacgcgcgc ctgggccgcg
     1981 gcggtcaacg agtccggcga ggcctatctg acgcctgcgg tcatcgacgg gcgctgggcg
     2041 gtgc
//
LOCUS       DATDEU010000389         9013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_656136, whole genome shotgun sequence.
ACCESSION   DATDEU010000389 DATDEU010000000
VERSION     DATDEU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9013
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656136"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VIV63_09245"
     CDS             <1..765
                     /locus_tag="VIV63_09245"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEY6124825.1"
                     /translation="AITGEDALSWRGYLAAHRKRRAFFAVMGATSTDHGHPTARTADL
                     SPADAEELFARIVRGAQHPGDADLFRGQMLTEMARMSLDDGLVLQIHPGAWRNHNAPL
                     FDKFGANVGADIPTATDYVGALRPLLNRFGNDPALSIIVFTLDESSYARELAPLAGHY
                     PALKLGPAWWFHDSPEGMMRFREQTTETAGFYNTVGFNDDTRAFLSIPARHDVARRVD
                     CSFLARMVAEHRLPEDEAATLAPQLAYEFPKKAYKL"
     gene            793..2331
                     /locus_tag="VIV63_09250"
     CDS             793..2331
                     /locus_tag="VIV63_09250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6124826.1"
                     /translation="MSSDNVTNDTGGNALRAAAGRVGNYRWVICALLFFCTTINYLDR
                     NSLSVLKTTLQGALGWTDVDYGWITFAFTFAYAAFPSLIGVFVDRFGVKKALAGALVL
                     WSLAAAAHGLVATVLGFVIVRFVLGIAEAANFPASIKAVGMWFPQKERAFATGIFNSG
                     TSVGVMVSFLTVWIAGHWGWQGAFVFIGVIGLVWLYFWQRFFDVPEKQARLGQAELDY
                     IKAGLPAVEKAVKVPWTSLLRYREIWPFIIGKFITDPVWWFYLFWLPSYLERERGQNP
                     LTSALWIGVIYTGSSIGSILGGWLSGAMIKRGWPVGKARMTTMLLAAVFMPGSILAYY
                     ADSFGMCVAFITLATACHQAWSANLFTNATDMFPGKVAGSVVGLGATAGGIGGMFMTL
                     LAGLAVQWTGNQQIVFVWAGGMHLLALALFWFWFKGRFVQVNVDAGNDETVAHRGLVI
                     AGAAVTAFGFALSALVYSNWQTMVTAVKATGAAQAAVVAVGFVVIGAALLYAGTPKKN
                     QVAA"
     gene            complement(2394..3143)
                     /locus_tag="VIV63_09255"
     CDS             complement(2394..3143)
                     /locus_tag="VIV63_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY6124827.1"
                     /translation="MSEPRRLYEQIAQQLARAIADGKYEIGQRLPSERELAQTFDVSR
                     PTVREAIIALELDELVEVRLGSGVYVVNREPPAGAAGYKDIGFFELLEARRAIEGEAC
                     AMAAMRIDDAQLAQLSALIDEMRDDNRRDEILLSEDADRRFHELIASSTQNTGITAAV
                     QMMWDSRVRSPQNRSMSAKVRARGYKPPIEEHAAIVRALKRRDPDAARAAMREHLSRV
                     IEDLLEVTEVEEMEKARAVAAEKRRRYSSTK"
     gene            3358..6411
                     /locus_tag="VIV63_09260"
     CDS             3358..6411
                     /locus_tag="VIV63_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6124828.1"
                     /translation="MLFKTKYQLAVLGYVGAAAFFTTTASAQNADASVPAKDSEEIEE
                     VVVTGFRASLQSSVGAKRDNIAFSDSIFAEDIGKFPDLNLAESLQRIPGVQIARDFTG
                     EGTSVAVRGLGKDFTQITLNGAPVETASDSNIDNVSQGRGLDLVLFPTELFRQLTVSK
                     TPTAKQQEGAVAANIDLRVARPFDEKGFHINYSAKGSFQESSEEWSPRLGFYASNTWD
                     TGMGEFGILGGVAYSNRSYRSDGFNTIGFTTVSLGNRCLYATDPQCNSFVTPNNETGY
                     GNQAQAWPTTVPAGYSFGTNGANAGDPLTVCGAGDTAGGTSNLSCRDLSFAIWPRLAR
                     PDMLIGERETTSGILSMQWAPSDKLEFYIDSLISKADHGYERNDLNLAIRSTNTNIPL
                     NVVLNEDNVVTSATIANPVWLNENRPYHEDTDFINLNGGMKWQIGEKFTVDASINYNH
                     SDWFRSTNTYLFNSNLNTGITVDLEANGDGVIKVTPSRDLNDLNFWNWNALRVQPVQR
                     ELWQKGGRVGGEWAFADAFKLGAGFSRDTFHREITNWETTSCATDGGNATSPSGRECA
                     AALTAAGIQHARVTVPNAQLGNYMQPWTHGTLYQTSDFDVGVNNGWALPNYRLLDPAT
                     NLDFFEKELAVGLVGGINVAGYNPRVLDEETDAFYLEANGQLEVLGNLRYNAGARYIK
                     TDQFVSGYISANTPAGAVRTYVEDSADYDKVLPSFNVASELGHGFVLRAAWSETMTRA
                     QPGDIAPNQALSINGDVLTIGNAALTPYFSDNYDLGLEWYFGESGLGMVAVNAWRKEI
                     EGYTTIVGTVTPFGDLGIDYSTLPIATQTGIQNTANAQCGCTTGNPLTANVRVDQRQN
                     TSEIITLDGWELTYVQPLDFLLKGAGFTVNYTHIEQESEGGLPGAPSSAITGLSPYTY
                     NVTAFYENYGFSGRLTYSVRDAFVEFLGNNENNIAGDNYAQERKYLDASFSYKLPMDT
                     DISISLEVQNILNQQLLTYFRSDEFTPRASFAPGRQLLLGISGSF"
     gene            complement(6473..7414)
                     /locus_tag="VIV63_09265"
     CDS             complement(6473..7414)
                     /locus_tag="VIV63_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY6124829.1"
                     /translation="MTTRREVIPLLAGAAGLSAASDSQAAPAVLSEPELKRVSYKSER
                     TGKERDYFVFLPRGFAQSKQWPVMLFLHGDGERGDAKGELDYVLIHGPLFEAWSQRRN
                     LPFVIISPQLPKFSRGDVEYIKNRTRDQIPQRLANWINPRPDAAAVRLPEPMQGRLSD
                     DKLPDGPEGPPDGWNEIESELIAMVDRTVADFKGDPRRVYLTGLSYGGFGAWNLAARH
                     PEKFAALVPIVGYAHPDHAEPIAKAKLPLWVFAGGRDPVVPLRYFYPALNKMEALGHP
                     EVRFTVEEDLGHLAWVRIYEGQDVYSWLLQHTKSRSD"
     gene            complement(7443..8549)
                     /locus_tag="VIV63_09270"
     CDS             complement(7443..8549)
                     /locus_tag="VIV63_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY6124830.1"
                     /translation="MTDNILNIRPASETKWDCASFGEVMLRFDPGFGRVRNARQFNVW
                     EGGGEYNVARAFKKCWGKRSTAVTALPVNDLGWLVEDLMMQGGVDTSKIIWRDFDGLG
                     RNTRVGLNFTEKGFGIRAALGCSDRGHSAASQIKPGEVNWEKLFGEEGVRWFHTGGIF
                     AALAPNTSEAVIEAVEIARKYGTIVSYDLNYRASLWKSQGGKEGAQKINRNIAKYVDV
                     MIGNEEDFTACLGFEVEGLDEHISAIDPANFKKMIQTAVKQFPNFKVAATTLRNAKTA
                     TFNDWSAILYAGGNFYQSMMRENLEIYDRVGGGDGFASGLAYGFMEGKGPQAAVEYGA
                     AHGALAMTTPGDTSMVNVKEVEAVMKGKGARVIR"
     gene            complement(8550..>9013)
                     /locus_tag="VIV63_09275"
     CDS             complement(8550..>9013)
                     /locus_tag="VIV63_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124831.1"
                     /translation="IMGVGSIVDAPTAGIYIANGAKFVVGPILNADVAKVCNRRKIPY
                     SPGCGSASEISYAEELGCEIVKVFPGSSVGGPDFVKAVLGPMPWTRIMPTGGVDPDEA
                     SVKKWFGAGIVAAGMGSKLITQELLDAGDYAGITKKVRETVDLIKKVRGKA"
BASE COUNT         1633 a         2878 c         2774 g         1728 t
ORIGIN      
        1 gccatcaccg gtgaagatgc gctgtcctgg cgtggatatc tcgctgccca tcgcaagcgc
       61 cgcgcgttct tcgcggtcat gggcgcgacc tccacggatc atggacatcc gacagctcgc
      121 accgcggact tgagtccggc cgacgccgaa gaactgttcg cccgaatcgt gcgcggcgcc
      181 caacatcctg gcgatgcgga tctcttccgc ggtcagatgc tcaccgaaat ggcgcgcatg
      241 agcctcgacg atgggctcgt gctgcaaatc catcccggcg cctggcgaaa tcacaacgcg
      301 ccgctgttcg acaagtttgg cgccaacgtc ggcgcggaca tcccgacggc aaccgattac
      361 gtcggtgcgc ttcgcccgct gctcaaccgg ttcggtaacg atccggcgct ttccataatc
      421 gtgttcacgc tcgacgaaag ctcttatgcg cgtgaacttg cgccgctggc aggtcactat
      481 ccggcgctca agctgggacc cgcctggtgg ttccacgaca gtccggaagg aatgatgcgc
      541 ttccgcgaac aaactaccga aaccgccggg ttctacaaca cggtcggctt caacgacgac
      601 acgcgcgcat tcctgtccat tcccgcgcgg catgacgtcg cccgtcgtgt cgattgttcg
      661 ttcctcgctc gcatggttgc cgagcaccgt ttgccggaag acgaggcggc gacgctcgcg
      721 ccgcagctcg cttacgaatt cccgaaaaaa gcgtacaaac tttgagcgat gaatccctgg
      781 ggggtctgcc gaatgagttc tgacaacgta acaaatgaca ccggtggcaa tgcacttcga
      841 gccgccgcgg gccgcgtagg caactaccgc tgggtgatct gcgcgctgct cttcttctgc
      901 accaccatca actacctcga ccgcaactcg ctgtcggtgc tcaagaccac tctgcagggc
      961 gcgctcggct ggaccgatgt cgactatggc tggatcacct tcgcgttcac cttcgcctac
     1021 gcggcgtttc cctcgctcat cggcgtattc gtcgaccgct tcggcgtgaa gaaggcgctg
     1081 gccggcgcgc tggtgctgtg gtcgctggct gctgccgccc acggactcgt ggccacggtg
     1141 cttggtttcg tcatcgtgcg tttcgtgctc ggcatcgccg aggcggctaa cttcccggcc
     1201 tccatcaagg ccgtcggaat gtggttcccg cagaaggaac gtgcgttcgc caccggcatc
     1261 ttcaactcgg gcaccagtgt cggtgtcatg gtgtcgttcc ttactgtgtg gatcgccgga
     1321 cactggggct ggcagggcgc cttcgtgttc atcggcgtca tcggcctggt gtggctgtat
     1381 ttctggcaga gattcttcga cgttcccgaa aaacaggctc gcctcggcca ggccgagctc
     1441 gactacatca aggcaggtct tcccgccgtc gagaaggccg tgaaggtgcc gtggacctca
     1501 ctgctgcgtt accgggaaat ctggcccttc atcatcggaa aattcatcac cgatccggtg
     1561 tggtggttct acctgttctg gttgccttcg tacctcgaac gcgagcgcgg ccagaatccc
     1621 ttgaccagtg cgctgtggat cggcgttatc tacaccggct cgtccatcgg ctcgatcctc
     1681 ggcggctggt tgtcgggcgc aatgatcaag cgcggctggc cggtcggaaa ggcgcgcatg
     1741 accaccatgc tgctggctgc ggtgttcatg ccgggctcca tcctcgcgta ttacgccgac
     1801 agcttcggca tgtgcgttgc gttcatcacg ctcgccaccg cctgccacca ggcgtggtcc
     1861 gccaacctgt tcacgaacgc aaccgacatg tttccgggca aggttgccgg gtcggtcgtc
     1921 ggcttgggcg ccaccgcggg cggcatcggc ggcatgttca tgactttgtt agcaggcctc
     1981 gccgtgcagt ggaccggcaa tcagcagatc gtgttcgtgt gggcgggtgg catgcacctg
     2041 ctggcgctcg cgctgttctg gttctggttc aagggccggt tcgtgcaggt gaacgtcgat
     2101 gcgggtaacg acgaaaccgt ggcccatcgc ggcctggtga ttgccggcgc ggccgtcacg
     2161 gcgttcgggt tcgcgttgtc cgcactggtc tacagcaact ggcagaccat ggtcacggcc
     2221 gtcaaggcga cgggtgcggc gcaggccgcc gtcgtcgcgg tcggcttcgt cgtgatcggc
     2281 gccgcgctgc tgtatgcggg gacgccgaag aagaatcagg tcgctgcctg agctggatgg
     2341 tgtcgcgggc cagaacgaat cgttcgggcc cgcggcgctg attgtgcctt cggctacttg
     2401 gtcgacgagt agcgccggcg tttctcggcg gccaccgcgc gggccttctc catttcctcg
     2461 acctcggtga cctcgagcag gtcctcgatg acgcgcgaga ggtgctcgcg catggcggcg
     2521 cgtgcggcat ccggatcgcg ccgcttcaat gcgcgcacga tggcggcgtg ttcttcgatc
     2581 ggcggtttat agccccgggc acgcaccttg gcgctcatgg agcggttctg tggcgagcgc
     2641 acacgcgagt cccacatcat ctggaccgcg gcggtaatac cagtgttttg ggtggagctg
     2701 gcgatgagct cgtgaaaccg gcgatcggca tcctcgctca gcaggatttc gtcacgacgg
     2761 ttgtcatcgc gcatttcatc gatgagcgcg gagagttggg ccagctgcgc atcgtcgatt
     2821 cgcatggccg ccatcgcgca ggcttcgccc tcgatggcgc gcctggcctc gagcagctcg
     2881 aagaagccga tgtccttgta gccggccgcg ccggcgggcg gctcccgatt caccacgtaa
     2941 acgcccgagc ccaggcgcac ctcgacgagt tcgtcgagtt cgagggcaat gatggcttcg
     3001 cgcaccgtcg gccttgatac gtcaaaggtt tgcgccagct cgcgctccga tggaaggcgc
     3061 tgcccgatct cgtatttgcc gtcagcaatc gccctggcga gctgctgcgc aatttgttcg
     3121 tagaggcgcc gtggttcgga catggcccgc agtctgcctg tagccacccc cgccaacaac
     3181 cgaaattggt aaggacaatt acggcattgg gagacaaata gcaggacagg gtggtcaggg
     3241 ggctacttgc agttggtctg accacttgct acggtgccac catcgttgca aagacaacgc
     3301 gagcgaccgc cgacacgatc gatggcgccg ggtttcttaa atcgaggggg atttcaaatg
     3361 cttttcaaaa ccaaatacca acttgcggtg ctgggatatg tcggcgctgc cgcgttcttc
     3421 accacgacgg cgtcagcgca gaatgccgac gcctccgttc ccgcgaagga ctcagaagag
     3481 atcgaggaag tggtcgtcac cggcttccgc gcttcgctgc agagctcggt gggcgcgaag
     3541 cgcgacaaca tcgcgttctc ggactcgatc tttgccgaag acatcggcaa gttcccggac
     3601 ctgaacctcg ccgaatctct gcagcgcatt cccggcgtgc agatcgcgcg cgacttcact
     3661 ggcgaaggca cctcggttgc cgtgcgcggc ctcggcaagg atttcacgca gatcacgctg
     3721 aacggcgcgc cggtcgaaac cgcctccgac agcaacatcg ataacgtgag ccagggccga
     3781 ggactcgacc tggttctgtt cccgaccgaa ttgttcaggc agctcaccgt gagcaagacg
     3841 ccgaccgcca agcagcagga aggtgccgtt gcggcgaaca tcgacctgcg cgtcgcgcgt
     3901 ccgttcgatg agaagggctt ccatatcaac tacagcgcca agggcagctt ccaggagtcg
     3961 agcgaagagt ggagcccgcg tctcggcttc tacgcgagca acacctggga cacgggaatg
     4021 ggcgagttcg gcatcctcgg cggtgtcgcc tattcaaacc gctcgtatcg ttcggacggt
     4081 ttcaacacca tcggcttcac caccgtttcg ctgggcaacc gctgcctgta tgccacggac
     4141 ccccagtgca acagcttcgt cacgcctaac aatgagacgg gctatggcaa ccaggcacag
     4201 gcgtggccga cgacggtccc cgccggatac agcttcggca ccaacggtgc gaatgcaggc
     4261 gatccactga cggtttgcgg cgccggtgac actgctggcg gaacctcgaa cctgtcctgc
     4321 cgcgatctta gtttcgccat ctggccgcgc ctcgcgcgcc cggacatgtt gatcggtgag
     4381 cgcgaaacca cttctggaat tctctcaatg cagtgggcgc cgtcggacaa gcttgagttc
     4441 tacatcgaca gtttgatctc gaaggccgac cacgggtacg agcgcaacga cctcaatctc
     4501 gccattcgca gcacgaatac aaacattccg ttgaacgtgg tgctgaacga agacaatgtc
     4561 gtcacgagcg cgaccatcgc caacccggtc tggcttaacg aaaaccgccc gtatcacgag
     4621 gatacggatt tcatcaacct caacggcggc atgaagtggc agatcggcga gaagttcacc
     4681 gtcgacgcgt ccatcaacta caaccacagt gactggttcc gttcgaccaa cacctacctc
     4741 ttcaattcga acctcaacac cggtatcacc gtcgacctcg aagccaatgg cgacggcgtc
     4801 atcaaggtca cgccgagccg cgacctgaac gacctcaatt tctggaactg gaacgcgctg
     4861 cgcgttcagc ccgtccagcg cgagttgtgg cagaaaggcg gccgcgtggg cggcgagtgg
     4921 gcattcgccg acgcgttcaa gctcggagcc ggcttctctc gcgacacctt ccatcgtgag
     4981 atcaccaact gggaaacgac gagttgcgca acggacggcg gcaatgccac gagcccctct
     5041 ggccgcgagt gcgccgccgc gctgaccgcc gctggcatcc agcacgcgcg agtcacggtt
     5101 cccaatgcgc agctgggcaa ctacatgcag ccctggaccc atggcacgct ctaccagaca
     5161 tcggacttcg acgtgggcgt gaacaatggc tgggcgttgc ctaactaccg cctgctcgac
     5221 ccggcaacta acctcgattt cttcgagaag gagcttgcgg tcggcctggt cggtggcatc
     5281 aacgtcgccg gctacaaccc gcgcgtgctc gacgaggaga cggatgcctt ctacctcgaa
     5341 gccaacggtc agctcgaagt cctcggcaac ctgcgataca acgcgggcgc acgctacatc
     5401 aagacggatc agttcgtgtc gggctacatc agtgcgaaca cgcccgccgg cgcggtgcgc
     5461 acgtatgtcg aagactcagc cgactacgac aaggtgctgc cgagcttcaa cgtggcgagc
     5521 gagctcggcc acggattcgt attgcgcgcc gcctggtccg agacgatgac tcgtgcccag
     5581 ccgggcgaca tcgctcccaa ccaggcgtta agcatcaacg gcgacgtgtt gaccatcggc
     5641 aacgccgcgc tcaccccgta cttctccgac aactatgacc tgggactcga gtggtatttc
     5701 ggtgagtccg gtcttggcat ggtcgccgtg aacgcgtggc gcaaggagat cgagggatac
     5761 acgacgatcg tcggaactgt cacgccgttc ggcgacctgg gtatcgacta ctcgacgctg
     5821 ccgatcgcca cgcagaccgg catccagaac acggcgaatg cgcagtgcgg gtgcacgacc
     5881 ggcaacccgc tgaccgccaa cgttcgtgtc gatcagcgac agaacaccag cgagatcatc
     5941 acgctcgatg gctgggaact gacctacgtt cagccgctcg acttcctgct gaagggcgcc
     6001 ggcttcacgg tgaactacac ccacatcgag caggagtccg aaggcggcct gccgggtgcg
     6061 ccgtcgagcg ctatcactgg tctttcgccc tacacctaca acgtcacggc cttctacgag
     6121 aactatggtt tctcaggccg cctgacctac tcggtgcgcg atgcgttcgt ggaattcctc
     6181 ggaaacaacg aaaacaacat cgccggtgac aactacgcgc aggagcgcaa atacctcgat
     6241 gcgtcgttca gctacaagct gccgatggac acggacatct ccatcagcct tgaagtgcag
     6301 aacatcctga accaacagtt gctgacgtat ttccgcagcg acgaattcac gccgcgtgcg
     6361 tcgttcgctc ccggccgcca gctcctcctg ggaatcagcg gttcgttctg agcgatcggc
     6421 acgattgcct gcaagcgatc aaggggaggc aacgcaagtt gcctcccttt tttcagtcgg
     6481 atcgcgattt cgtgtgctgc agaagccagg aatagacatc ctggccttcg tagatgcgca
     6541 cccaggcaag gtgtcccagg tcttcctcaa cggtgaagcg cacttcggga tggccgagcg
     6601 cctccatctt gttgagggcg gggtagaagt aacggagcgg cacgacgggg tcgcgcccac
     6661 ccgcgaacac ccagagcggt agtttggcct tggcgatggg ttcggcgtgg tcggggtgcg
     6721 cgtagccaac aatgggcacg agcgccgcga atttctccgg atggcgcgcg gcgagattcc
     6781 acgcgccgaa tccgccgtaa ctcagcccgg tcagatacac ccggcgtgga tcgcccttga
     6841 agtcggcaac cgtgcgatcg accatggcga tcaactcgct ctcgatctcg ttccacccat
     6901 ccggcggccc ctccgggcca tccggcagct tgtcgtccga gagtctgccc tgcatgggct
     6961 ccggtagccg cacggcggcg gcatccggcc tcgggttgat ccagttggcc aaccgctgcg
     7021 gtatctggtc gcgcgtgcgg ttcttgatgt actccacgtc accccggctg aacttcggga
     7081 gttgcggcga gatgatcacg aaaggcagat tgcggcgctg gctccacgct tcgaacagcg
     7141 gcccgtggat cagcacgtaa tcgagttcac ccttcgcgtc gccgcgctcg ccgtcgccgt
     7201 gcaggaacag catgaccggc cattgcttcg actgggcaaa accgcgaggc agaaagacga
     7261 agtagtcgcg ctccttgccg gtgcgctcgc tcttgtagga aacgcgtttg agctcgggct
     7321 ccgagagcac cgcgggcgcg gcttgcgaat cggaagcggc cgaaagaccg gccgctcccg
     7381 caagcagggg gatcacttct cttcgtgtcg tcatgaaagc ccctctggag tgcgctcgac
     7441 cttcagcgga tgacccgggc tcccttgccc ttcatcaccg cctcgacttc cttgacgttg
     7501 accatcgagg tgtcgcccgg cgtggtcatg gcgagcgcgc catgcgcggc gccatattcc
     7561 acggcagcct gcggaccctt gccctccatg aatccatagg cgaggcccga cgcgaatccg
     7621 tcgccgccac ccacgcgatc gtagatctcg aggttctcac gcatcatcga ttgatagaag
     7681 ttgccgcccg catagaggat ggccgaccag tcgttgaacg tcgcggtctt cgcattgcgc
     7741 agcgtggtgg ccgctacctt gaagttcggg aactgcttga ccgccgtctg gatcatcttc
     7801 ttgaagttgg ccggatcgat ggccgagatg tgctcatcga gaccttcgac ttcgaagccc
     7861 aagcaggccg tgaaatcttc ttcgttgccg atcatcacgt cgacgtactt agcgatgttg
     7921 cggttgatct tctgcgcgcc ttccttgcca ccctggctct tccagaggct ggcgcggtag
     7981 ttgaggtcgt acgacacgat ggtgccgtac ttgcgcgcaa tctcgacggc ctcgatgacc
     8041 gcctcggagg tgttgggcgc gagcgcggcg aagatgccgc cggtgtggaa ccagcgcacg
     8101 ccttcctcgc cgaagagctt ctcccagttc acttcaccgg gcttgatctg tgacgcggcg
     8161 gaatgtccac ggtcggaaca gccgagcgcg gcgcgaatgc cgaagccttt ctcggtgaag
     8221 ttcaggccca cgcgcgtgtt gcggccgagg ccatcgaaat cgcgccagat gatcttcgag
     8281 gtgtcgacgc cgccctgcat catcaggtct tcgaccagcc agcccaggtc gttgaccggg
     8341 agagccgtga cggcggtgga gcgcttgccc cagcacttct tgaaggcgcg ggcgacgttg
     8401 tattcgccgc cgccttccca gacgttgaac tggcgggcgt tgcgaacccg gccaaaaccc
     8461 ggatcgaagc gcagcatgac ttcgccgaac gaagcgcagt cccacttggt ttccgaagcg
     8521 gggcggatgt tgaggatgtt gtcggtcatc taggctttcc cacgaacctt cttgatcagg
     8581 tctacggtct cacggacctt cttggtgatg ccggcatagt cgccagcgtc gagcagttcc
     8641 tgggtgatga gcttggagcc catgccggcg gcgacgatgc ccgcgccgaa ccacttcttg
     8701 accgaggctt cgtccggatc gacgccgccc gtcggcatga tgcgggtcca gggcatgggg
     8761 cccagcaccg ccttgacgaa atcggggccg ccgaccgacg agccggggaa caccttgacg
     8821 atctcgcagc ccagttcttc ggcgtaggag atttccgagg ccgaaccgca gccgggcgaa
     8881 taggggatct tgcgacggtt gcagaccttg gcgacgtcgg cgttgaggat cgggccgacc
     8941 acgaacttgg cgccgttggc gatgtagatg ccggcggtgg gcgcatcgac gatgctgccg
     9001 acgcccatga tca
//
LOCUS       DATDEU010000390         6600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_363149, whole genome shotgun sequence.
ACCESSION   DATDEU010000390 DATDEU010000000
VERSION     DATDEU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363149"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VIV63_09280"
     CDS             complement(<1..1070)
                     /locus_tag="VIV63_09280"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY6124832.1"
                     /translation="MRERGIALLLALASLLAFYGLWLRPEPSLDPDANIARPTTAEHR
                     GNGYAALYEWLQRSGVGVRSLRDRYAALADLDVPPRGNLLVLSLPAVEVFRSEELSAL
                     DKWIRRGNTLLINAALLDQPAWAVRRSAGAVVEIEALTAIEFETRKAREARLDDTPLS
                     QRVREADERDAKRKGDEDEEEVEDEDAEDEFGMKPNELLDVPEKIELTATGPHVLLAG
                     VKSLMLETDYEAQEWSLRMPYDNFVLTLARTADGEGALFEQRVGEGRVLLSAGGSLFT
                     NRALGNADNSRLFANIVGACMTADGVVLFDDLRQGLSASYDPARFYDDPRLYKTMFIV
                     LGLWLVWVLGSTRLRAPSIETH"
     gene            complement(1070..1999)
                     /locus_tag="VIV63_09285"
     CDS             complement(1070..1999)
                     /locus_tag="VIV63_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY6124833.1"
                     /translation="MRSWLALLGLVACGVAMSADDAALRTVEACRARLDPRVDIGIER
                     IQKRCPDLLPALASAPWRDLLPKTLRERREEISAESLRALEELVRQSTTGAAPRPALS
                     RERLDPVLAELGEKGQQGATRWERFKRWLKEKLENRQDDDDSNWIERLARQFKTSEGV
                     AQFITYAGYVMVGVLVLFVIVSELRAAGLLGGTRRASARANAAAEWRRRLVLGDVANA
                     PLAERPGMLLRLLGEALTRAQRLPAADGLTATAIAQRARLDSADEREELRMVATTAEV
                     VRYGELPPSDDAIEGTVATARSLLDKLARQGKR"
     gene            complement(1996..2772)
                     /locus_tag="VIV63_09290"
     CDS             complement(1996..2772)
                     /locus_tag="VIV63_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124834.1"
                     /translation="MTAEVLDAAYRLFRAGIFRCLPYSGLAVLVLELPTLYSTFLVSP
                     DGASRIESPWVKLAAYSILFLLSVALLGVITLRLTAVEQGKRPRFRQELAVVLWRLPA
                     AALATLFAFAYPLMMFLLAPVLMFTLPTEALVFAAVPLLWPVALFVMALPAFWCDGLG
                     PFAAIGQAIRVSMRRSWRLVGAIVTTGLMLMAFTLLPVTVAAMLSPMFGRVDLFLVAT
                     IASMFYLVLGAFGVPFVLAVLIVAYQDLKLRDREWRGARS"
     gene            2835..3683
                     /gene="prmC"
                     /locus_tag="VIV63_09295"
     CDS             2835..3683
                     /gene="prmC"
                     /locus_tag="VIV63_09295"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY6124835.1"
                     /translation="MHHRCPMLDLSDSSRLLANAARTIDRRDAEILLAACWRKSRSQL
                     LAAIGEAVPAAVADCFENARRQRADGVPVAYLLGHREFWSLEFTVNDAVLVPRPETEL
                     LVQRVLDLVSSPTAAVADLGTGSGAIAIALANERPGWRVLGTDASDRALAVARGNGDR
                     LAGGRVEWIQGDWFAALAGRRFDALVSNPPYVAGNDPVLVGDGLRHEPRSALTPEGDG
                     FAALVTLINGAPGHLQPGGWLALEHGATQGETLRSLLVARGFTHVTSHRDLAGHERVT
                     EGNLAP"
     gene            3702..4184
                     /locus_tag="VIV63_09300"
     CDS             3702..4184
                     /locus_tag="VIV63_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6124836.1"
                     /translation="MFETTLGNFEVELFEKEAPISTANFLEYVDDGFFDGVIFHRVIP
                     DFMIQGGGFEPGMKQKKVKAPIKNEATNGIKNARGTLAMARTNDINSATAQFFVNLKD
                     NGFLDHSGPGNFGYAVFGKVVSGMDVIDQIADVATTTRSGHQNVPAKDVVITRASRKV
                     "
     gene            4181..4945
                     /gene="mtgA"
                     /locus_tag="VIV63_09305"
     CDS             4181..4945
                     /gene="mtgA"
                     /locus_tag="VIV63_09305"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY6124837.1"
                     /translation="MKKSLGRRLVRWTLIGLAAAFLVTALPVLAMRWMDPWYSAFMLD
                     AALDASRAGKTNYQTDYRWVDLDRISPHAAVAVIAAEDQFFPFHVGFDFKSIREAVRS
                     NEAQSKKRRPRVRGASTISQQVAKNLFLWSGRSYVRKGLEVYFTLLIELTWPKERILE
                     VYLNVAQFGDGIYGVEAAAQRFYRMPAARLGRYESATLAAVLPNPITFKVQAPSNYVT
                     KRRDWILAQMRGLGGPRYLNELENPTEPPKARATSR"
     gene            5039..6157
                     /locus_tag="VIV63_09310"
     CDS             5039..6157
                     /locus_tag="VIV63_09310"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HEY6124838.1"
                     /translation="MNENPYERASPVSPSSPAEPVTRVLLPFEFRGNGGEYFRIWIVN
                     LLLTIVTLGIYSAWAKVRRLRYFYGNTVVDGHTFEYHGQPLAILKGRLIVVAGYVVFL
                     ISSQMFPIFSALFALALAIFGVPWIIMRSRKFQMRMTSYRNLRFNFHGEYGAAMSAFI
                     GWYFLALITIGILFPVWVRKRVQYSLDNAAYGNQKFTFLTGNGTYFRFCLATFFMGIA
                     AYLLVIFGVFAGGDLKSLSYPGADPSALEVMNAIGPAGWLLMIGGIVALLGITGYYQA
                     SFANAAFGGVQCGAQHGRSNLKAWPLIGIYLSNLVAIVLTLGLFYPWAKVRQVRYQLE
                     NTAIDSDGNLAAFTASSGVGIDAVGEEVGDFFDVDFGL"
     gene            6159..>6600
                     /locus_tag="VIV63_09315"
     CDS             6159..>6600
                     /locus_tag="VIV63_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124839.1"
                     /translation="MIAGRYFDGRSAAGVDASLDFGADGNVHVHGAASPITVPLAEIR
                     ISERVGNITRRITFPDGGVFETQDNDAVDQARDALGMRGGSKLVHWLESRWHVAVGSL
                     VAVVLVSIAFVHWGVPAVANWAADVMPTEVDQAIGAGSLDVLDRV"
BASE COUNT         1163 a         2276 c         2043 g         1118 t
ORIGIN      
        1 tcgtgggtct cgatcgacgg cgcgcgaagt ctcgtcgaac cgagcaccca gaccagccac
       61 aggccgagca ctatgaacat cgtcttgtac agccgcgggt cgtcgtagaa gcgcgcaggg
      121 tcgtaactcg cggaaagtcc ctggcgcaga tcgtcgaaca gcaccacgcc atcggccgtc
      181 atgcacgcgc cgacgatgtt ggcgaacaac ctggaattgt cggcattgcc cagcgcgcgg
      241 ttcgtgaaca gcgatccgcc cgcgctcaac aagacgcgcc cttctccgac gcgctgctcg
      301 aacagcgcgc cttcgccgtc cgccgtgcgc gccaacgtga gcacgaaatt gtcatacggc
      361 attcgcagcg accactcctg cgcttcgtaa tccgtttcga gcatcagcga cttcacaccg
      421 gcgagcagca catgcggccc ggtcgcggtc agctcgatct tctcgggcac atcgagcaat
      481 tcgttgggct tcatgccgaa ctcgtcctcg gcgtcttcgt cttcgacctc ttcttcgtcc
      541 tcatcgcctt tgcgtttcgc atcgcgctcg tcggcttcgc gaacccgctg cgacagcggt
      601 gtgtcatcga gtcgggcctc gcgcgccttg cgggtctcga attcgatcgc cgtcagcgct
      661 tcgatttcga ccaccgcgcc cgccgaacgc cgcaccgccc aggccggctg atcgagcagc
      721 gcggcattga tgagcagcgt gttgccgcgg cggatccact tgtcgagcgc gctgagttcc
      781 tccgagcgaa acacttccac cgcgggcaac gacaagacca gaaggttgcc gcgcggcggc
      841 acatcgaggt cggccagcgc cgcataccgg tcccgcagcg aacgcacacc cacgcccgac
      901 cgttgcagcc attcgtacaa cgcggcgtag ccattgcccc gatgctcggc cgtggtgggc
      961 cgcgcaatgt tcgcgtccgg atcgagcgac ggctccgggc gcagccagag cccgtagaac
     1021 gccagcagcg acgcgagcgc cagcagcagt gcgatgccgc gttcccgcat cagcgttttc
     1081 cctggcgcgc gagtttgtcg agcaacgacc tggcggtggc gaccgtgccc tcgatggcat
     1141 cgtcagacgg cggcaactcg ccgtaacgca ccacttcggc ggtcgtggcg accatgcgca
     1201 gctcttcgcg ttcatccgcc gagtccagac gggcacgctg ggcgatcgcg gtggcagtca
     1261 gtccgtccgc ggccggcagc cgctgcgcgc gcgtcagcgc ctcgcctaac aacctcaaca
     1321 acatgccggg cctttcggcc agcggagcat tggcgacgtc cccgagcacc agccgccggc
     1381 gccactcggc cgctgcattg gcgcgggcgg atgcgcggcg ggttccgccc aggagacccg
     1441 cggcgcgaag ttccgacacg atgacgaaca acaccagcac cccgaccatc acgtagcccg
     1501 cataagtgat gaactgcgcc acgccctcgc tggtcttgaa ctggcgggcc aggcgctcta
     1561 tccagttcga atcgtcgtca tcctggcgat tctcgagctt ctccttgagc catcgcttga
     1621 agcgttccca ccgcgtagca ccctgctggc ccttctcgcc gagttcggcc agcaccggat
     1681 cgaggcgctc gcggctgagc gcggggcgcg gggccgcacc ggtggtcgac tgtctcacca
     1741 gctcctcgag cgcgcgcaga ctttcggcgg agatttcctc gcgccgttcg cgcagggtct
     1801 tcggcagcag atcgcgccac ggcgcgctcg ccagcgcagg cagcaggtcc gggcagcgct
     1861 tctggatgcg ctcgatgccg atgtcgacgc ggggatcgag gcgcgcgcga caggcttcca
     1921 cggtccgcaa cgccgcgtcg tcggccgaca tcgcgacacc gcaggcgacc agccccagca
     1981 gtgcaagcca tgacctcacg accgcgctcc gcgccattcg cgatcgcgca acttcaggtc
     2041 ctggtacgcg acgatgagca ccgcgagcac gaacggcacg ccgaaggcgc cgagcaccag
     2101 gtagaacatc gaagcgatcg tggccacgag gaagaggtcg acgcggccga acatcggcga
     2161 cagcatcgcc gcgaccgtca ccggcagcag cgtgaacgcc atcagcatga ggcccgtggt
     2221 gacgatggcg ccgaccagcc gccacgagcg ccgcatcgac acgcgtattg cctggccgat
     2281 cgcggcaaag gggcccaatc cgtcgcacca gaatgccggc agcgccatca cgaacaaagc
     2341 cacgggccac agcaacggca ccgcggcgaa aaccagcgcc tccgtcggaa gcgtgaacat
     2401 cagcaccggc gccagcagga acatcatcag tggataggcg aacgcaaaga gggtcgcgag
     2461 cgcggcggcc ggtaaacgcc agagcaccac cgcgagctcc tgtcggaaac gcggccgctt
     2521 gccctgctcg accgcggtga ggcgcagcgt gatcacaccg agcaacgcca cgctcaacag
     2581 gaacaggatg gaataggcgg cgagcttcac ccacggactc tcgatgcgcg acgcgccgtc
     2641 gggcgacacg agaaaggtcg aatacaacgt gggcagttcg agcaccagga ccgcgagccc
     2701 ggaataaggc aggcagcgga agatgccggc gcggaacagg cgatacgccg catccagcac
     2761 ctctgctgtc atcagcggcc gggcatatag cgcgcggtac atgcacggca tgataccgat
     2821 gccggaccga agtcgtgcat catcgctgcc ccatgctcga cttgtccgac agcagccggc
     2881 tcctggcaaa cgccgcgcgc accatcgacc gccgcgacgc ggagatcctg ctcgccgcct
     2941 gctggcgcaa gagccgctca caacttctgg ccgcgatcgg cgaagccgtg cctgccgcgg
     3001 ttgccgactg cttcgaaaac gcgcgccggc agcgcgcgga cggcgtgccg gtcgcctatc
     3061 ttctgggcca ccgcgagttc tggtcgctcg agttcacggt gaacgacgcc gtgctcgtgc
     3121 ctcgtcccga gaccgagctg ttagtgcaac gtgtgctcga tctcgtgagc tcgcccaccg
     3181 cggcggtcgc cgatctcggc accggctcgg gcgccattgc catcgcactg gcgaacgaac
     3241 gtccgggctg gcgcgtgctc ggcaccgatg cctcggatcg ggcgctcgcg gtggcgcgcg
     3301 gcaacggcga tcgtctggcg ggtggtcgcg tggagtggat ccagggtgac tggttcgccg
     3361 ctctcgccgg tcgccggttc gacgcgctgg tcagcaatcc gccgtatgtc gcgggcaacg
     3421 atccggtgct cgtcggagac ggcctgcgcc acgagccccg atcggcgctg actccggaag
     3481 gcgacggatt cgcggccctg gtcactctta ttaatggcgc ccccgggcat ttgcagcccg
     3541 gcggctggct ggcgctcgaa cacggcgcca cgcagggcga aacgctgcga tccctgcttg
     3601 tcgcccgtgg cttcactcac gtaacctcgc accgcgatct cgccggccac gagcgcgtca
     3661 ccgaaggcaa tctggcccca taggaaactt aagtgtcact agtgtttgaa accacgctcg
     3721 gcaatttcga agtcgagctg ttcgagaaag aagctccgat ctccaccgcg aattttctgg
     3781 agtacgtcga cgacggattc ttcgacggcg tcattttcca tcgcgtcatc ccggacttca
     3841 tgatccaggg cggcggcttc gagccgggca tgaagcagaa aaaggtcaag gcgcccatca
     3901 aaaatgaggc gaccaacgga atcaagaatg cgcgcggcac tctcgccatg gcgcgcacca
     3961 atgacatcaa cagcgccacc gcgcagtttt tcgtcaacct gaaagacaac ggctttctcg
     4021 atcactcggg cccgggcaac ttcggttacg ccgtgttcgg caaggtcgtc agcggcatgg
     4081 acgtcatcga ccagatcgcc gacgtcgcga ccaccacgcg ttcgggacac cagaacgtgc
     4141 cggccaagga cgtggtcatc accagggcct cacgaaaagt gtgaagaaga gtctcgggcg
     4201 gcggctggtc cgatggactc tcatcgggct cgccgccgcc tttctcgtta cggcgctgcc
     4261 cgtcctggcc atgcgctgga tggacccgtg gtacagcgcc ttcatgctcg acgcggcgct
     4321 cgacgcctcg cgcgccggca aaactaacta ccagaccgac tatcgctggg tggatctcga
     4381 ccggatctcg ccgcacgccg ccgtggccgt gatcgccgcc gaagaccagt tctttccgtt
     4441 tcacgtgggc ttcgatttca agtcgatccg cgaggcggta cggtcgaacg aggcgcaatc
     4501 gaagaagcgc cggccgcgcg tgcgcggcgc cagcaccatc agccagcagg tggccaagaa
     4561 cctgttcctg tggtcgggca ggagctacgt gcgcaagggc ctcgaggtgt acttcacgct
     4621 gctcatcgag ctcacgtggc cgaaggaacg catcctcgag gtgtatctga acgtggcgca
     4681 gtttggcgac ggcatctatg gcgtggaagc cgcggcacaa cggttctacc gcatgccggc
     4741 cgcgcgcctc ggccggtacg aaagcgccac tctcgctgcc gtgctgccga acccgatcac
     4801 cttcaaggtg caggcgcctt ctaactatgt aacaaagcgg cgcgactgga tcctggcgca
     4861 gatgcgcggt ctcggcggcc cacggtatct gaacgagctc gaaaacccga ccgaaccgcc
     4921 gaaagcccgt gccacgagtc gatgaactgt gacacctgaa atgcactggc ttcccgagtc
     4981 ctgcagggat taacctagtc tccggatcgc cgtcggcgat cttccaggga gaataataat
     5041 gaacgagaac ccgtacgaac gtgcgagccc tgtctcgccc tcttccccag ccgaacccgt
     5101 gactcgcgtg ctgctgccat tcgaattccg cggcaacggc ggcgaatact tccgcatctg
     5161 gatcgtcaac ctgctgctca ccatcgtcac gctcggaatc tattccgcgt gggccaaggt
     5221 gcgccggttg cgctacttct acggcaacac cgtcgtcgac gggcacacgt tcgaatacca
     5281 cggccaaccg ctcgccatcc tgaaaggccg gctcatcgtg gtcgccggct acgtcgtgtt
     5341 cctgatttct tcgcagatgt tcccgatctt ctccgccctg ttcgcgctcg ccctcgccat
     5401 cttcggcgtg ccctggatca tcatgcggtc gcgcaaattc cagatgcgca tgacttcgta
     5461 ccgcaacctg cgcttcaact tccacggcga atacggcgcc gccatgtcgg cgttcatcgg
     5521 ctggtatttc ctggcgctga tcaccatcgg catcctgttc ccggtctggg tgcgcaagcg
     5581 cgtgcagtac tcgctggaca atgccgccta cggcaaccag aagttcacgt tcctcacggg
     5641 caatggcacg tacttccggt tctgcctggc cacgttcttc atgggcatcg ccgcctatct
     5701 tctcgtcatc ttcggtgtct tcgctggcgg cgacctgaag tcgctcagtt atccgggcgc
     5761 cgatccctcc gccctcgaag tcatgaacgc catcggcccg gcgggctggc tgctgatgat
     5821 cggcggcatc gtcgccctgc tgggcatcac cggctactac caggcatcgt ttgccaacgc
     5881 ggcgttcggt ggcgtccagt gcggcgcgca gcacgggcgt tctaacctca aggcctggcc
     5941 gctcatcggc atctacctgt cgaatctcgt ggcgattgtc ctcacactgg gcctgttcta
     6001 cccgtgggcc aaggtgcggc aagtgcgtta ccagctcgaa aacacggcaa tcgattccga
     6061 cggcaatctc gcggctttca ccgccagctc cggcgtaggc atcgacgcgg tgggtgaaga
     6121 agtcggcgac ttcttcgacg tggacttcgg gctttgacat gatcgccggc cgctatttcg
     6181 acgggcgaag tgcggctggc gtcgacgcca gcctcgactt cggcgccgac ggcaacgtgc
     6241 atgtgcacgg cgcggcctcg cccatcaccg tcccgctcgc cgagatcagg atctccgagc
     6301 gggtcggcaa tatcacgcgc cgcatcacct ttccggacgg tggcgtgttc gagacgcagg
     6361 acaacgatgc ggtcgaccag gcgcgcgacg cgctcggcat gcgcggcggt tcgaagctcg
     6421 tccactggct cgaaagccgc tggcatgtcg ccgtcggctc tctcgtggcc gtggtgctgg
     6481 tatcaatcgc gttcgtgcac tggggagtcc ccgccgtcgc caattgggct gccgacgtca
     6541 tgcccaccga agtcgaccag gcgatcggcg ccgggtcgct cgacgtgctc gatcgcgtcg
//
LOCUS       DATDEU010000391         7564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_72567, whole genome shotgun sequence.
ACCESSION   DATDEU010000391 DATDEU010000000
VERSION     DATDEU010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72567"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VIV63_09320"
     CDS             <1..781
                     /locus_tag="VIV63_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEY6124840.1"
                     /translation="RPDAEPLRMILRDAIFSTDRRHLGAQAAPAPALAPDAPPLPLNE
                     VHELGATYEARPADVQPLSSDRVASWLVTQDGATRTALAAMLSDELAVVRDAAHEEGY
                     ARGHTEAMKEAREKVGAAIAAIESVHEAAEVAFATETEQFGEQCADLVAEVFFKLAGT
                     ALVTREAIVGVVGEVVKRVREERELRIHVNPLDLPVLRTAEAGIAASLPGRQFTLVAD
                     GRVASGGCIVESALGSLDGRLEVQLAELCATLRDAKQRGPA"
     gene            778..2157
                     /locus_tag="VIV63_09325"
     CDS             778..2157
                     /locus_tag="VIV63_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEY6124841.1"
                     /translation="MSRMHALSEQLRGGDWVRRIGRIVRFVGLTLESSGPDARIGEIC
                     EIHARGRRGVTLAEVVGFSEQRVLLMPFGDLQGIEIGSEVIATGRTADVAAGEELLGR
                     VIDGFGQPLDGAPLVAPALRYPLYPPPLNPLQRGMVREVLETGIRAIDTLLTLGRGQR
                     VGIFAGSGVGKSTVLGMLAREVKADVSVIALIGERGREVRAFVEEGLSEEARKRSVVV
                     AATSDQPPLVRRRAAFLATAVAEHFRDAGLHVCLTMDSITRVAMAQREIGLAAGELPT
                     ARGYTPSVFTMLPRLLERGGVHSAGGSITGLYTVLVEGDDVNEPISDSVRSILDGHIV
                     LSRDIAHRGRYPAIDVPRSISRLASGLITEGDRAVVADAVKLLATYESSRDLIEVGAY
                     RAGTTPSVDRAIALIPSIEQFLAQRPAETESRAAACARLESLLAAAVQDSKAKMPRLT
                     IPAISAVGA"
     gene            2154..2612
                     /locus_tag="VIV63_09330"
     CDS             2154..2612
                     /locus_tag="VIV63_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124842.1"
                     /translation="MTDRKQKRLDLLRRLREMTVEQARAEHVAAQTELEQRQEKADDT
                     ERRLKSLDQWSLDRLSLGAPLVPELLRQAQLFRGVEKTTLEKQRAEEASQREATEAAR
                     GELTARFEELSVVERLTNRHVQAVTDEHIRRGFVDLDEAGVLAKNLESKE"
     gene            2615..3031
                     /locus_tag="VIV63_09335"
     CDS             2615..3031
                     /locus_tag="VIV63_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEY6124843.1"
                     /translation="MAIDAIGLAGSSDAGVPANASVNQTDFLKILLTQLRFQDPLKPV
                     DNQQFVAQLAQFSALEINRQQSEKIDTLLQISSSGQAIGLLGQRVEVSGASGGGVGEV
                     FAVSFATGEPRLSVRTEGNPIVGARLVDIKLVQEGP"
     gene            3049..4239
                     /locus_tag="VIV63_09340"
     CDS             3049..4239
                     /locus_tag="VIV63_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY6124844.1"
                     /translation="MGLSGMNAYAKGLDVISNNVANLNTTGFKEGIASFSNVVSRSGG
                     GATTGSAGASTSGGGVQVNAESQNFSQGDRQSTNQSLDAALDGNGFFVLERDGQRYYT
                     RAGQFVLDKDGILTEQITGARVMMSSEGSSMGALNIDPFRTFPPRATTEVKLSGNLAR
                     SGTDLPTIPVLEVIDTAGNKQSIKIRFVRNAEDPLLWQVEVLNAVDVSLGSGALRFNA
                     DGTPSADNSPITATVTPENLPPFTFTLNFGGAGSYGGMTSLLNNPASAPNLLKQDGLQ
                     FGTFEGANFDERGNLEATYSNQEKKKIGRLVLASFDSSGDLTSVGNGMYVTQEGRSPK
                     LSAGMEFGLGRVEGGKLEMSNVELTDQFTTLIILQRGYQACSQMTSVANEMMQQLLSM
                     QSGR"
     gene            4236..5051
                     /locus_tag="VIV63_09345"
     CDS             4236..5051
                     /locus_tag="VIV63_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HEY6124845.1"
                     /translation="MKAPPLLFLGDRRREAVITRIAAASRRWRQMWSPGADDAFEAVC
                     DAPAVGGFSEPVASVATTCWSIDVGNERTAVLLLPHSTFAWVVHEGAGALPPDSAGMQ
                     APDSVTGKLEYEVARTLLTELCAPEIREPVSVTRLPADALPDWSRDTRAWSLHVKAPA
                     SGRGFCLLVSAARLELLAPARAVVADKSLAARRDAIGDHTVALRAVVGETSLSINELA
                     DLALDDVLVLDQHLSEPVSLVGAPSGTPVGAGNLGRAGSRRAIKVAGVNAQRN"
     gene            5064..5375
                     /locus_tag="VIV63_09350"
     CDS             5064..5375
                     /locus_tag="VIV63_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEY6124846.1"
                     /translation="MTSKVVNSIDLPILHAGGPNAQSRVGERLDLIEHVKVRVSVSLG
                     ETEIPISRLFALGGGDVLALDRDADAPVDIRVNDRVIARGVLVAVGDQFGVRITEIES
                     R"
     gene            5376..5780
                     /locus_tag="VIV63_09355"
     CDS             5376..5780
                     /locus_tag="VIV63_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124847.1"
                     /translation="MHNAKVISRVFIAWLAAPLAQAADPAEKLRGFGLAESSAASPGY
                     GRVVIAFLLVAAMAWGIVWVLRRYGLRGPAAATGLGSSVRPLARTSLPGGITCHVIET
                     QGRQVLITVTRHGVTSLVLGDAAAPSIPGPAP"
     gene            5777..6511
                     /gene="fliP"
                     /locus_tag="VIV63_09360"
     CDS             5777..6511
                     /gene="fliP"
                     /locus_tag="VIV63_09360"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY6124848.1"
                     /translation="MRKLLLPLLLLGIAGGAWGAESAPLTLSLDAGAKGGSVSDALRI
                     VMLLTLLAVLPAIMIAMTSFTRTIIVLSMLRHAFGMQDTPPNMVLVSLALFLTLFTMA
                     PAFQSSYQNGIKPLADNQITMESAVTLAVRPLREFMIRQTREKDLALILEIAKAEVPD
                     SIEDVSTIHIIPAFLISELKTAFTIGFVIFLPFLLLDLVVSSVLMSMGMLMVPPTIIS
                     LPLKVLMFVLIDGWSLIVRALLGSFH"
     gene            6522..7277
                     /locus_tag="VIV63_09365"
     CDS             6522..7277
                     /locus_tag="VIV63_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6124849.1"
                     /translation="METFRPEFPVEAADPESDRARWVEFARTRAAELRTALIERYIGQ
                     AKIMAAKLYQRRQDNSVAFGDYLQYARVGLIESIDHFDPAREVPFEAFSSYRIKGAIL
                     NGLQTESEIASQRAFWSRRARERFESLKDHEVKGDRRASLEELVNLTMGLALSHLIEQ
                     DAGEVIDESLAANPYAVTELVQLKRAVRSLLPALPSRERELIQRHYEEQVEFQQIAAE
                     WGLTKGRVSQLHAQALQRLRKLLSTQPQIDETL"
     gene            complement(7295..>7564)
                     /locus_tag="VIV63_09370"
     CDS             complement(7295..>7564)
                     /locus_tag="VIV63_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124850.1"
                     /translation="AAEPRAAIAGAEPLRDRLRQRLQSLGNVDRRRAREAFVETVLAT
                     ELGDNITLDPAMAEIVGKIAAQLGSDAATDRDLGVLFDELAREKA"
BASE COUNT         1284 a         2443 c         2534 g         1303 t
ORIGIN      
        1 tcgaccagat gcggagccgc ttcgcatgat tctgcgcgac gccatctttt cgaccgaccg
       61 gcggcatctc ggcgcgcagg ccgcgcctgc tccggcgttg gcgccggatg cgccgcccct
      121 gccgctgaac gaagtgcatg agctcggcgc gacgtatgag gcgcgtcctg ccgacgtgca
      181 gccgctgtcg tcggatcgcg tcgcgagctg gctggtcacg caggacggcg cgacccgcac
      241 cgcgctcgct gccatgttgt ccgatgaatt ggccgtggta cgcgatgccg cccacgaaga
      301 gggttatgcg cggggccaca ccgaagcgat gaaggaggcg cgcgaaaagg tcggcgccgc
      361 catcgcggcg atcgaatccg ttcacgaagc cgccgaagtg gccttcgcca ccgagacgga
      421 acaattcggc gaacaatgcg ccgacctcgt tgccgaagtg ttcttcaagc tggccggcac
      481 ggcgctcgtg acgcgcgaag ccatcgtcgg tgtggtgggc gaagtcgtga agcgcgtgcg
      541 cgaggagcgc gagctgcgta tccacgtgaa cccgctcgac ctgccggtgt tgcggaccgc
      601 cgaggcgggc atcgccgcga gcctcccagg ccgccagttc acgctggtgg cggacgggcg
      661 cgtggcctcg ggcggctgca tcgtcgagtc ggcgctcggc agcctcgatg gccggctcga
      721 agtgcagctc gccgagctct gcgccacgtt gcgcgatgcg aagcagcgag ggccggcatg
      781 agccgcatgc acgcactctc ggagcagctt cgtggcggcg actgggtgcg gcgcatcggc
      841 cgcatcgtgc ggttcgtcgg gctcacgctc gagtcgagcg gccccgacgc gcgtatcggc
      901 gagatctgcg agatccatgc gcgcggacgt cgcggcgtca cgctcgcgga ggtcgtgggt
      961 ttctccgagc agcgtgtgct gctcatgcct ttcggcgatc tgcagggcat cgagatcggc
     1021 agcgaagtca tcgctaccgg tcgcactgcg gatgtcgcgg ccggtgaaga attgttgggc
     1081 cgcgtgatcg acggcttcgg ccagccgctc gacggcgcgc cgctcgtggc gccggcgctg
     1141 cgatacccgc tgtacccgcc gccgctcaat ccattgcagc gaggcatggt gcgcgaagtg
     1201 ctcgagaccg gcatccgcgc catcgacacg ctgctgacgc tcggccgcgg gcaacgtgtc
     1261 ggcatcttcg cgggcagcgg cgttggcaag agcacggtgc tcggcatgct ggcgcgcgag
     1321 gtcaaggccg acgtatcggt catcgcattg ataggcgagc gcggccgcga agtgcgcgcg
     1381 ttcgtcgaag agggcctctc ggaagaggcc cgcaagcggt cggtggtggt cgccgcgacg
     1441 tcggatcagc ccccgctggt ccggcgtcgc gcggcttttc tcgccaccgc cgtcgccgaa
     1501 cattttcgcg acgcgggatt gcacgtgtgc ctcacgatgg actccatcac gcgcgtggcg
     1561 atggcgcagc gcgagatcgg tctcgcggcg ggcgagttgc ccaccgcgcg tggttacacg
     1621 ccgtcagtgt tcacgatgtt gccgcgcctg ctcgaacgtg gcggcgtgca cagcgcgggc
     1681 ggctcgatca ccggcctgta caccgtgctg gtcgaaggcg acgacgtgaa cgagccgatc
     1741 tccgattcgg tgcgttcgat cctcgacggt cacatcgtgt tgtcgcgcga tatcgcgcac
     1801 cgcggccgtt acccggccat cgacgtgccg cgcagcatca gccggctggc ctcagggctc
     1861 atcaccgaag gcgatcgcgc ggtggtggcc gatgcggtca agcttttggc gacctacgag
     1921 tcgtcgcgcg atctcattga agtcggtgcg tatcgcgccg gcaccacgcc gtcggtggac
     1981 cgcgcgattg cgctcatccc gtcgatcgag cagttcctcg cgcagcgtcc cgccgaaacc
     2041 gaatcgcgcg ccgcggcctg cgcgcgcctc gaaagcctgc tggccgcggc cgtgcaggac
     2101 tcgaaggcga agatgccgcg cctgaccata ccggccatca gcgcggtggg cgcatgaccg
     2161 atcgcaagca gaagcgactc gacctgctgc gccgcctgcg cgaaatgacc gtcgaacagg
     2221 cgcgtgccga acacgtggcc gcgcagaccg agctcgaaca gcgacaggag aaagccgacg
     2281 acaccgagcg gcggctgaag tcgctggatc agtggtccct cgaccggctt tcgctgggtg
     2341 cgccgctggt tcccgaacta ttgcgccagg cgcagctctt tcgcggcgtg gagaagacca
     2401 ccctcgaaaa gcaacgcgcc gaggaagcca gccagcgcga agccaccgaa gccgcgcgcg
     2461 gcgaactgac cgcgcgcttc gaagaactgt ccgtggtcga gcgcctgacg aaccgccacg
     2521 tgcaggccgt gaccgatgaa cacattcgcc gcggtttcgt cgatctcgac gaagccggtg
     2581 tgctcgccaa gaacctcgag tccaaggagt aaacatggcc atcgatgcaa tcggattagc
     2641 cgggtcgtca gatgcaggcg tgccggcaaa cgcgtccgtc aatcagacgg actttctcaa
     2701 gatcctgctc acgcagcttc gattccagga tccgctcaaa ccggtggaca atcagcagtt
     2761 cgtcgcccag ctcgcgcagt tcagcgcgct ggagatcaac cgccagcaga gcgagaagat
     2821 agacacgctg ttgcagatca gctcgtcggg ccaggctatc ggcttgctgg gccagcgggt
     2881 cgaggtgagc ggcgcatcgg gcggcggcgt gggcgaggtc ttcgcggtgt cgttcgccac
     2941 cggcgagcca cggctctcgg tgagaactga agggaatccg atcgtcggcg cgcggctggt
     3001 cgatatcaaa ctcgtgcagg agggaccctg aggttaggca ctatctctgt gggtctctcg
     3061 ggcatgaatg cctatgcgaa aggcctcgac gtcatcagca acaatgtggc caacctcaat
     3121 accaccgggt tcaaggaagg catcgccagc ttctccaacg tggtctcccg cagcggtgga
     3181 ggagctacga ctggcagcgc cggcgcctcg accagtggcg gcggcgtaca ggtgaacgcc
     3241 gagtcgcaaa atttcagtca aggcgacagg caatccacca atcagtccct cgatgccgcg
     3301 ctcgacggca acggtttctt cgtactggag cgtgatggcc agcgctatta cacgcgcgcc
     3361 ggacagttcg ttctcgacaa ggacggcatc ctcaccgagc agatcacggg cgcccgggtg
     3421 atgatgagca gcgaaggctc gtcgatgggt gcgctgaata tcgatccctt ccgtacattc
     3481 ccgccgcgcg cgactaccga agtgaagctg tcgggaaacc tcgcgcgttc gggaactgac
     3541 ttgcccacca tccccgtgct cgaggtcatc gatacggccg gcaacaagca gtcgatcaag
     3601 atccgcttcg tgcgcaacgc ggaagacccg ctgctgtggc aggtcgaagt gctcaacgcc
     3661 gtcgacgtgt ctctcggcag cggcgcgctg cggttcaatg cggacggaac gccttcagcg
     3721 gacaactctc ccatcacggc cacggtgact cccgagaatc tcccgccctt cacgttcacg
     3781 ctgaacttcg gcggcgctgg gagctatggc ggcatgacca gcttgctcaa caatcccgcc
     3841 tctgcgccca atctgctgaa acaggatggc ctgcaattcg ggacgttcga aggagccaac
     3901 ttcgatgaac gtggcaacct cgaagcgacc tactcgaacc aggagaagaa gaagatcggg
     3961 cggctggtat tggcgagctt cgattcgagc ggcgatctga cgtcagtcgg caacggaatg
     4021 tacgtcacgc aggaaggccg ctcgccgaaa ctgtccgccg gcatggagtt cggtctcggc
     4081 cgcgtcgaag gcggaaaact ggaaatgtcc aacgtcgaac tgaccgatca gttcaccacg
     4141 ctcatcatcc tgcagcgcgg ctaccaggcg tgctcgcaga tgacgtcggt ggccaacgag
     4201 atgatgcagc aactgctgtc gatgcagagc ggccgatgaa agcgccgccg ctgctgttcc
     4261 tgggcgaccg tcgacgcgag gcggtgatca cgcgcatcgc ggcggccagc cgccgctggc
     4321 gacagatgtg gtcgccgggc gcagacgacg ccttcgaagc ggtctgcgac gcgccggccg
     4381 tcggaggatt ctctgagccg gtggcgtccg tggcaacgac ttgctggtcg atcgatgtgg
     4441 gcaacgagcg cacggccgtg ttgctgcttc cgcacagcac gttcgcgtgg gtggtacatg
     4501 aaggcgctgg cgcgctgccg cccgattcgg cgggaatgca ggcgcctgat tccgtcaccg
     4561 gaaaactgga gtacgaagtc gcgcgtacgt tgctgaccga gttgtgcgca cccgaaatac
     4621 gggagcccgt gagcgtgacg cgattgccgg cggatgcgct gccggactgg agccgcgata
     4681 cgcgcgcctg gtcgttgcac gtaaaagcgc cggcatcggg ccgcggattt tgcttgttgg
     4741 tgagcgcagc aagactggaa ctgctggcgc cggcacgcgc cgtggtcgcc gacaaatcgc
     4801 tggcggcccg gcgtgacgcc atcggtgacc acaccgtagc gctgcgagcg gtcgtcggcg
     4861 agacctcgct gtccatcaac gaactcgcgg atctcgcgct cgatgacgtc ctggtcctcg
     4921 accagcatct gtcggagccg gtgtcgctcg tgggcgcgcc gtccggcacg ccggtgggcg
     4981 cgggcaatct cggacgcgcg ggttcacgcc gcgcgatcaa ggtcgccggc gtcaacgcac
     5041 aacggaatta aaggagtatt gaaatgactt cgaaggtagt gaacagcatc gatctgccca
     5101 tcctgcacgc gggcggtccc aacgcgcagt cgcgcgtggg cgagcggctc gatctcatcg
     5161 aacacgtgaa ggtgcgcgtg agtgtgtcgc tgggcgaaac cgagattccg atctcgcgcc
     5221 tgtttgcgtt gggcggcggc gatgtgctgg cgctcgatcg ggatgcggat gcgcccgtcg
     5281 acattcgcgt caacgatcgg gtcatcgcgc gcggcgtgct cgtggccgtc ggcgaccagt
     5341 tcggcgtgcg catcaccgag atcgaatcgc gctgaatgca taacgcgaag gtcatatcgc
     5401 gggtgttcat cgcatggctc gcggcgccgc tcgcgcaggc ggccgatccg gcggagaaac
     5461 tgcgcggatt cggcctcgcc gaatcgtccg cggcctctcc ggggtatggc cgcgtcgtca
     5521 tcgcgttcct gctggtcgcc gcgatggcct ggggcatcgt gtgggtgctg cgccgctacg
     5581 gcttgcgtgg cccggcggcc gcgaccggtc tcggctcgtc ggtgcgcccg ctcgcgcgta
     5641 cctcgctgcc cggcggaatc acctgtcacg tcatcgagac gcagggccgg caagtgctca
     5701 tcacggtgac acgccatggc gtcacctcgc tggtactcgg cgacgccgcg gcgccctcca
     5761 ttccaggacc tgcgccatga ggaagctgct gctgccattg ttgctgctgg gaatcgccgg
     5821 cggcgcctgg ggtgcggaga gcgcgccgct cacgctctca ctcgatgcgg gcgcgaaggg
     5881 cggttcggtc tccgatgcgc tgcgcatcgt gatgctgctg acgctgctcg cggtgttgcc
     5941 ggccatcatg atcgcgatga cgtcgttcac ccggactatc attgttctgt ccatgctgcg
     6001 ccatgcgttc ggcatgcagg acacaccgcc taacatggtg ctggtgagtc tcgcgctgtt
     6061 cctcacgctg ttcaccatgg cgccggcttt ccagtcgtct taccagaacg gcatcaagcc
     6121 gctggccgac aatcagatca ccatggaatc cgcagtgacg ctggcggttc ggccgctgcg
     6181 tgaattcatg attcgccaga cgcgcgagaa ggacctggcg ctgatcctgg agatcgcgaa
     6241 agccgaagtg cccgattcca tcgaagacgt ttcgacgatc cacatcatcc cggcgtttct
     6301 catcagcgag ctcaagacgg ccttcaccat cggcttcgtg atcttcctgc cgttcctgct
     6361 gctcgacctc gtggtctcga gcgtgctcat gtcgatgggc atgctcatgg tgccgccgac
     6421 catcatctcg ctgccgctca aggtgctgat gttcgtgctc atcgacggct ggtcgctcat
     6481 cgttcgcgcg ctgctcggca gttttcactg acggcgccag cgtggaaacc ttccgccccg
     6541 agtttcccgt cgaagcggcc gacccggaga gcgatcgcgc acgctgggtc gagttcgcgc
     6601 gcacgcgcgc ggcggaactg cgcaccgcgc tgatcgagcg ttacatcggc caggccaaga
     6661 tcatggcggc gaagctctac cagcgcaggc aggacaactc cgtggctttc ggcgattacc
     6721 tgcaatacgc gcgcgtcggg ttgatcgaat ccatcgatca cttcgacccc gcgcgggaag
     6781 taccgttcga agcgttctcg agttatcgga tcaagggggc gatcctcaac ggcttgcaga
     6841 ccgaatccga gatcgcttcg caacgcgcgt tctggtcacg ccgggctcgc gaacggttcg
     6901 agtcgctcaa ggaccacgag gtcaagggcg accgccgcgc atcgctcgag gagctggtga
     6961 atctcacgat gggcctggca ttgagccacc tcatcgagca ggacgccggt gaggtcatcg
     7021 acgaatcgct cgcggccaat ccctatgcgg tcacggagct ggtgcaattg aaacgcgcgg
     7081 tgcgctcatt gttgccggcg ctgccgtcgc gcgagcgcga gctcatccag cggcactacg
     7141 aagagcaggt cgagtttcaa cagattgcgg cggagtgggg cctgaccaag ggccgcgtgt
     7201 cgcaacttca cgcacaggcc ctgcaacgcc tgagaaagct tctcagcaca cagccgcaga
     7261 tcgacgaaac cttgtgaggc ggtgcgcgcg gccctcaggc tttttcgcgc gccagctcgt
     7321 cgaacaacac gccgaggtcg cgatccgtcg ctgcatcgct gccaagctgt gcggcaatct
     7381 tccccactat ttccgccatg gccgggtcga gcgtgatgtt gtcgccgagt tccgtggcca
     7441 gcaccgtttc gacgaaggcc tcacgcgcgc ggcggcgatc cacgttgccg agtgattgaa
     7501 ggcgctgccg aaggcgatcg cgcagcggtt ccgcgcccgc gatggcggcg cgaggctcag
     7561 cggc
//
LOCUS       DATDEU010000392         4018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1677741, whole genome shotgun sequence.
ACCESSION   DATDEU010000392 DATDEU010000000
VERSION     DATDEU010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677741"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..952)
                     /locus_tag="VIV63_09375"
     CDS             complement(167..952)
                     /locus_tag="VIV63_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6124851.1"
                     /translation="MTQRLSRTFFGTASLEREYQGFRVALRRGTVPPEAVPEHSHQTA
                     HLILAIDDEYLSGAVGATAWSGPSMLIYNPPGTLHRDRFATTGGRFLSIDFPQGFEPR
                     GVLDPVIIQRGAARLRVAEVLTALIEDAPVLEAEDRLLSLSAALAKEAEPTLSPPPWL
                     AIAIESIADLANDAGLRVHDIAQLAGVHPVHLARVFARHLGCSPGHAIQRARVERAAA
                     SMTRRGTLGDIAHECGFADQAHMTRCFRSLYGTTPTGFRAAFD"
     gene            complement(1105..3519)
                     /gene="gyrB"
                     /locus_tag="VIV63_09380"
     CDS             complement(1105..3519)
                     /gene="gyrB"
                     /locus_tag="VIV63_09380"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY6124852.1"
                     /translation="MANDDVYDSSKIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VVDNAIDEALAGHCDRIVVTIHADESVTVSDNGRGIPTDIHPEEGRSAAEVIMTVLHA
                     GGKFDDSSYKVSGGLHGVGVSVVNALSDNLWLTINRDGKAHKMEFRLGDPVAPLAIAG
                     DTTEKGTVVRFKASPTIFTNITFDYEILAKRLRELSFLNSGVRIELIDEREAKNDVFQ
                     HEGGLQEFVKHLNRSRTGIHPSVFWFRTQEGPIGVEVAMQWNDSYTESMFCYTNNIPQ
                     KDGGTHLSGFRAALTRTLNDYIEKEMAAKKEKVPTTGDDSREGLTAILSVKLPDPKFS
                     SQTKEKLVSSEVKGVVESAVAEKLSEFLLEHPNEAKAIVSKIIDAARAREAARKAREM
                     TRRKGALDIAGLPGKLADCQEKDPALCEIFIVEGDSAGGSAKQGRDRRTQAILPLKGK
                     ILNVEKARFDKMIQSAEVGTLITALGCGIGRDEFDINKLHYHRIIIMTDADVDGSHIR
                     TLLLTFFYRQIPLLIERGHIYIAQPPLYKVKRGKQEVYVKDDLELNTMLLASALDDAQ
                     LHVNSAAPPLSGQGLEALARQYMEVQAIIKRWARRYDERLLDQLIYMPSVSAAEFERA
                     DWMRDWTRDLAQHLNGLSDSPRSYRVELKQADGHSARVTVSKTEHGSVTEKALPKEFF
                     ESAEYRRIVELGKTLAGLIGDGAYIQRGEAREDIGSFKQVMQWLLDQARKGQSIQRYK
                     GLGEMNPDQLWDTTINPATRRLMQVRIEDILASDEIFTTLMGDQVEPRREFIEKNALA
                     VSNLDV"
     gene            complement(3546..>4018)
                     /locus_tag="VIV63_09385"
     CDS             complement(3546..>4018)
                     /locus_tag="VIV63_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124853.1"
                     /translation="IPCLEQNFERFGGLGVSVGFTTGWLAGTAFEDSLRTHADRDRAR
                     GTTTAGPHRADVSLRRNQRIARETLSRGQQKLTAVAMIVSQLRLLQDELNLRAVLLLD
                     DPAAELDDKNLHRLFEELTALRCQMIATSLTQETALFQAPKATFHVEQGRVIRV"
BASE COUNT          709 a         1200 c         1273 g          836 t
ORIGIN      
        1 ggactgcgca accgcggacg tggctgcggc gatcatgaga atggtggcca gtgcggcgtt
       61 gccgaacgca tgttgcatcg agtctctcgc tgagggtctg tgtgctgaca ccgtatctcg
      121 gcgacgctgc ggcgtcttga acggatgaaa catcgccggg caaatttcag tcgaatgctg
      181 cccgaaaacc ggtcggggta gtgccgtaca aagagcgaaa gcatcgcgtc atgtgtgcct
      241 gatcggcaaa gccacattcg tgagcgatat cgccgagggt cccgcgcctg gtcattgatg
      301 cagctgcgcg ctcaacgcgg gctcgctgaa tcgcatgccc aggcgaacat ccgagatgtc
      361 gcgcgaaaac ccgcgcgaga tggaccggat gtacgcccgc gagctgggcg atgtcgtgca
      421 cccgcagtcc agcgtcgttc gccagatccg cgatggattc gattgcgatg gcgagccagg
      481 gcggtgggga aagggttggc tccgcctctt ttgccaacgc ggcagacaaa ctgagcagac
      541 ggtcttcggc ttccagcacc ggtgcgtctt cgatcagcgc ggtcagcact tccgccaccc
      601 gcaggcgcgc ggccccccgc tggatgatca ctggatcgag gacgccccgc ggctcgaaac
      661 cttgtgggaa atcgatcgac aggaatcgcc cgcccgtagt cgcgaatcgg tctcggtgca
      721 acgttccggg cggattatag ataagcatgg atggcccgct ccacgccgtc gcgccgaccg
      781 ctccgctcag atattcgtcg tcgatcgcga ggatgaggtg cgctgtctga tgcgaatgct
      841 ccgggaccgc ttccggtggc acggtaccgc gccgcagcgc tacgcggaaa ccctggtatt
      901 cgcgttccag tgaggccgtg ccaaaaaaag tgcgcgaaag cctctgcgtc atcccaccac
      961 cctatgccgc tccgcttcac cttgccatca agtgcgcgct gctgaaagcg cccgacgaac
     1021 atctcgcgtg gcgtggcgga gcgttacaca actcgattag ttaggcggcg acacgcgaac
     1081 cgggatcctg cgagtcggcc gacctcacac gtcgaggttc gaaaccgcca gtgcgttctt
     1141 ctcgatgaat tcgcggcgcg gctccacctg gtcgcccatg agcgtggtga agatttcgtc
     1201 ggacgcgaga atgtcttcga tgcgcacctg catgagccgg cgcgtcgcgg gattgatggt
     1261 ggtgtcccag agctgatcgg ggttcatttc gccaaggccc ttgtagcgct ggatggactg
     1321 gcccttgcgc gcctggtcga gcagccactg catgacctgc ttgaacgagc cgatgtcctc
     1381 gcgcgcttcg ccgcgctgga tgtacgcacc gtcgccaatg agtccggcca gggtcttgcc
     1441 gagttcgacg attcggcggt actcagccga ctcgaagaat tccttcggaa gtgccttttc
     1501 tgtaacagaa ccgtgctctg tcttgcttac tgttacacgg gcggaatgac cgtccgcctg
     1561 cttgagctca acgcgatagc tgcgcggact gtcggacagg ccgttcagat gctgcgcgag
     1621 gtcgcgcgtc cagtcgcgca tccagtcggc gcgctcgaac tccgcggcgc tgaccgaggg
     1681 catgtagatg agctggtcga gcaagcgttc gtcatagcga cgcgcccaac gtttgatgat
     1741 ggcctgcact tccatgtact gccgcgcgag cgcttccagg ccctgacccg acagcggcgg
     1801 cgccgcgctg ttgacgtgca gctgtgcgtc gtcgagcgcg gatgccagca acatggtgtt
     1861 gagctcgaga tcgtctttga cgtagacctc ttgcttgccg cgcttcactt tgtagagcgg
     1921 tggctgtgcg atgtagatat ggccgcgctc gatgagcagc gggatctgcc ggtagaagaa
     1981 cgtgagcagc agcgtgcgga tgtgcgaacc gtcgacgtcc gcgtccgtca tgatgatgat
     2041 gcggtggtag tgcagcttgt tgatgtcgaa ttcgtcgcgg ccgatgccgc aacccagcgc
     2101 cgtgatgagc gtgccgactt cggccgactg gatcatcttg tcgaagcgcg ccttctcgac
     2161 gttcaggatc ttgcccttga gcggcaggat tgcctgggtg cgccggtcgc gaccctgctt
     2221 ggcggatcca cctgccgagt caccctcgac gatgaagatt tcgcagagcg ccgggtcttt
     2281 ctcctggcag tccgccagtt tgccgggcag gcccgcgata tcgagcgcgc cctttcgacg
     2341 cgtcatttcc cgcgcttttc tcgccgcttc gcgcgcgcgc gccgcgtcga tgatcttgct
     2401 gacgatggct ttcgcttcgt tcggatgttc gagcaggaac tcggaaagct tctcggcgac
     2461 ggcggattcc accacgccct tcacttccga cgagaccagc ttttccttgg tctgcgacga
     2521 gaacttggga tcgggtagtt tgacggagag gatcgcggta agaccttcgc gcgagtcgtc
     2581 gcccgtggtc ggaacctttt ccttcttcgc cgccatctct ttttcgatgt agtcgttcag
     2641 cgtgcgcgtg agcgcggcgc ggaaaccgga gagatgggtg ccgccgtctt tctgcggaat
     2701 gttgttcgtg tagcagaaca tcgactcggt ataggagtcg ttccactgca tggccacttc
     2761 gacgccaatc gggccttcct gggtgcggaa ccagaacacc gacggatgaa tgccggtgcg
     2821 cgagcggttc aggtgtttga cgaactcctg gagtccgccc tcgtgctgga agacatcgtt
     2881 cttcgcttcg cgctcgtcga tgagctcgat gcggacgccg gaattcagga acgaaagctc
     2941 gcgaagccgc ttggcaagaa tttcgtagtc gaacgtgatg ttcgtgaaga tggtcgggct
     3001 ggccttgaag cggacgaccg tgcccttctc ggtggtgtcg ccggcgatgg ccaggggcgc
     3061 cacgggatcg cccaggcgga attccatctt gtgagctttg ccatcgcgat tgatggtcag
     3121 ccagaggttg tccgacagcg cattgacgac cgagacgcca acgccgtgta aaccgcccga
     3181 gaccttgtac gagctgtcat cgaacttacc gccagcatgc agcacggtca tgatgacctc
     3241 ggctgcagag cgcccttctt cgggatggat gtcggtcgga atgccgcggc cgttatccga
     3301 aacggttacg gattcgtcgg cgtggatggt gacgacaata cggtcgcaat ggccggccaa
     3361 cgcctcgtcg atggcgttat ccacaacctc gaaaaccatg tggtgaagac cggtgccgtc
     3421 atcggtgtca ccgatgtaca tgccgggtct tttgcgcact gcatcaagac cctttaagac
     3481 cttgattttg ctggagtcgt agacgtcgtc gttggccatg actgactcgg gaaaacgggc
     3541 gcgaattata cccgtattac cctcccttgt tccacgtgaa acgtcgcttt aggtgcttga
     3601 aatagcgcgg tttcttgggt caacgacgtg gcgatcattt ggcatcgaag cgccgtcagt
     3661 tcttcgaaga gccgatgcag gtttttgtca tccagttcgg cagccggatc gtccaacagg
     3721 aggacggcgc gcaggttgag ttcgtcctga agcagccgaa gttgcgacac gatcatcgcc
     3781 acggccgtga gcttttgctg gccacgcgac agcgtctccc gggcgatgcg ctgattgcgc
     3841 cggaggctga cgtcggctct atgcgggccc gctgtggtgg tcccgcgagc acgatcccta
     3901 tctgcatggg tgcgaagtga atcttcgaag gcggtcccgg caagccaccc cgtggtgaat
     3961 ccgacgctca cgcccaagcc cccgaagcgc tcgaagttct gctcgaggca cggaatca
//
LOCUS       DATDEU010000393         5446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1095835, whole genome shotgun sequence.
ACCESSION   DATDEU010000393 DATDEU010000000
VERSION     DATDEU010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095835"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VIV63_09390"
     CDS             <1..661
                     /locus_tag="VIV63_09390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6124854.1"
                     /translation="AQVAFVDYAEGPSQELIQRVEKAGLPRPWYRKTDVTDIKALQTA
                     IRDAAQAIGDFHVLINNVASDDRHSLMDVTPEYYDARIAVNQRPAFFAIQAVVPGMKK
                     MGGGSIINFGSNSYGAKGRNMPVYTTAKSSVIGLTRGLAAELGEHRIRVNVITPGWIM
                     TQRQIDKWLTPEGELEIKRNQVLPDKVRPEDVAPMALFLASDDSRACSAQEYIVDAGW
                     L"
     gene            658..1653
                     /gene="araD1"
                     /locus_tag="VIV63_09395"
     CDS             658..1653
                     /gene="araD1"
                     /locus_tag="VIV63_09395"
                     /inference="COORDINATES: protein motif:HMM:NF040903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HEY6124855.1"
                     /translation="MRILQFTDGGHTRVARIDASGAVLPLTRFESTWQLARACIGERR
                     TLEQVASAEPVGPALSYDELLGEGRLLPPLTHPDPGHCLVSGTGLTHLGSAAARDAMH
                     TKLTKTTDELTDSMKMFKAGVEGGKPKSSKPGMQPEWFYKGNGRNVVGCGSPLASPDF
                     ALDHGEEPELVGLYIIDDNRRPHRLGFAIGNEFSDHVTERRNYLLLAHSKLRQCGVGP
                     MLNTGTLPAHLEGTSRIRRDGQVLWQKPFLTGEANMCHTFANLEYHHFKYKQHRVPGD
                     VHLHFFGTATVSVADNIVTQAGDVFEIELPALGAPLTNKLLIEPGGLALHETAAL"
     gene            1677..2639
                     /locus_tag="VIV63_09400"
     CDS             1677..2639
                     /locus_tag="VIV63_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6124856.1"
                     /translation="MMFIRRIVILAVATGALAAAGCSREKVDDRIVLGFSQIGAESEW
                     RTANTESIKSAALTSNIDLRFADAQQKQENQIKALRSFIAQKVDVIAFSPVVATGWDT
                     VLMEARAAGIPVILTDRAVTSDPSLYVGFLGSDFVEEGRKAGRWTLEKFKDIPGEVNI
                     VELQGTVGAAPANDRKKGFEEIIAADPRFQIIRSQTGDFTRTKGKEAMEAILKSESRK
                     IHLLYAHNDDMAIGAIQAIEEAGLKPGKEILIVSIDAVKGAFEAMIAGKLNATIECNP
                     LLGPQLMTSVTEVVSGRPIPKRIVVEETMFTMETAKQQMHTRKY"
     gene            2648..4180
                     /locus_tag="VIV63_09405"
     CDS             2648..4180
                     /locus_tag="VIV63_09405"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY6124857.1"
                     /translation="MNDPAIVLRAHDISRAFPGVQALSGASLTLRAGEVHALMGQNGA
                     GKSTLIKVLTGLCAPDSGSIELLGKVVSPQSPVHAQQLGISAVHQESHLLPNLSVAEN
                     ICAGRYPRHPWTRGGGIDWRETERRAAELLASLGIKLDVTQLAGGLPAALQQLATVAR
                     AVATDARVLILDEPTSSLDADEVQALFALIRRLRDKGVAILFVTHFLDQVYDICDRMT
                     VLRNGRFVGEYACAKLDAHGLVAAMVGRELSAPAAAPDHAAAGADPHSAAPPVLAARN
                     LSRRGQLKPLDIELHAGEVLGLAGLLGSGRTELARLLFALDRRDTGEITIDGKPADLD
                     SPADALRAGLGFCPEDRKDSGIVADLSVRENIALALQARMGLTKFLKVSDQRQIAEKM
                     IAALGIKTASIETPIGQLSGGNQQKAIIARWLATHPRVLILDEPTRGIDVAAKQELMN
                     EILELARGGTAVLFISAEIDEVVRVSDRILVLRDRARAGELPRGATEDAVYSLIAASH
                     EA"
     gene            4177..5196
                     /locus_tag="VIV63_09410"
     CDS             4177..5196
                     /locus_tag="VIV63_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6124858.1"
                     /translation="MKRLAQHALFWPAVTLALLLVASSLFNPSFLTLRWHEGHLYGSV
                     IDILNRAAPLIVVSLGMTLVIAVRGLDISVGAVVAISAAVAALMIGGSFSATGEVHSE
                     NPLWLALVTALGVAAACGLWNGLLVVKAGMQPIVATLILMVAGRGIAQLLTGGQIITV
                     YYPPFFTLGKGFLLGLPVALWIAALLWTSLHLVLSRTALGLFVRSIGGNPEAARVAGV
                     RSSSITLCVYTFCGLAAGIAGLIVSSNVKSADANNAGQLLELDAILAVTLGGTALTGG
                     RFTLAGTVIGALIIQTLTSMIYSLGVPPEVNLVVKAALVFAVMLLQSAEFRRMVAQLA
                     RGRAA"
     gene            5193..>5446
                     /locus_tag="VIV63_09415"
     CDS             5193..>5446
                     /locus_tag="VIV63_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124859.1"
                     /translation="MNRRLLPLTVTILLFVAMAAFGAIRFDGFLSMQVFLNLIIDNAF
                     LCIVAVGMTFVILSGGIDLSVGSIIALTTMILATLLNAGW"
BASE COUNT          974 a         1832 c         1722 g          918 t
ORIGIN      
        1 tgcgcaggtg gctttcgtcg actacgcgga aggcccttcg caggagctga ttcaacgggt
       61 cgaaaaggcc ggccttccgc ggccctggta tcgcaaaacc gatgtcaccg atatcaaagc
      121 gctgcaaacc gccattcgcg acgccgccca ggcaataggc gacttccacg tactcattaa
      181 taacgtcgcc agcgacgacc ggcattcact aatggacgtg accccggagt actacgatgc
      241 gcgcatcgcg gtgaaccagc ggccggcgtt cttcgccatc caggccgtgg tgccgggcat
      301 gaagaagatg ggcggcggct ccatcatcaa cttcggcagc aattcgtacg gcgccaaggg
      361 ccgcaacatg ccggtgtaca cgacggcgaa gtcttcggtc atcgggctca cgcgtggtct
      421 cgccgcggaa ctcggcgagc accgcatccg cgtcaacgtg atcacgcccg gctggatcat
      481 gacgcagcgg cagatagaca agtggctcac gcccgaaggc gagctcgaga tcaaacgcaa
      541 ccaggtgttg cccgacaagg tccggccgga agacgtggcg ccgatggcgc tgtttctcgc
      601 gtcggacgac tcgcgcgcct gctccgcgca ggaatacatc gtcgacgccg ggtggttatg
      661 agaattcttc aattcaccga cggcgggcac acccgcgtcg cgcgcatcga cgccagcggc
      721 gccgtgttgc cattgacgcg tttcgagagc acttggcaac tcgcgcgtgc gtgcatcggc
      781 gagcggcgca cgctcgagca ggtggcgagc gctgagccgg tcggcccagc gctgtcatac
      841 gacgaactgc tgggcgaagg ccggctgctg ccaccgctca cgcacccgga tcccgggcac
      901 tgcctcgtga gcggcaccgg gctcacgcat ctcggcagcg ccgcggcgcg cgatgccatg
      961 cacacgaagc tcacgaagac caccgacgaa ctcaccgact ccatgaagat gttcaaggcc
     1021 ggagtcgaag gcggcaaacc gaagagcagc aaacccggca tgcagccgga gtggttctac
     1081 aagggcaatg gccgcaacgt ggtgggctgc ggctcgccgc tcgcgtcacc cgacttcgcc
     1141 ctggatcacg gcgaagaacc cgaactcgtc ggtctctaca tcatcgacga caaccggcgc
     1201 cctcaccggc tcggattcgc catcggcaac gaattctccg atcacgtgac cgagcgtcgc
     1261 aactatctac tgctcgcgca ttcgaagctg cgtcagtgcg gcgtcggccc gatgctcaac
     1321 accggcacgc tgccggcgca cctggaaggc acgagccgca tacgccgcga cggccaggtg
     1381 ttgtggcaga agccgtttct caccggcgaa gcgaacatgt gccacacgtt cgccaatctc
     1441 gaatatcacc acttcaagta caaacaacat cgcgtgcccg gcgacgtgca cctgcacttc
     1501 ttcggcaccg cgaccgtcag cgtggccgac aacatcgtga cgcaggccgg cgatgtgttc
     1561 gaaatcgaac tgccggcgct cggagcgccc ctcacaaaca agttgttgat cgagccgggc
     1621 ggcctcgcgc tgcacgaaac cgcggcgctt tgaaagcgaa gccagggaga tccccgatga
     1681 tgttcatccg acgaatcgtc attctcgcgg ttgccaccgg tgcgctcgcg gccgccggct
     1741 gctcccgcga aaaggtcgac gatcgcatcg tgctcggctt cagccagatc ggcgccgaga
     1801 gcgagtggcg cacggcgaac accgaatcga tcaagtcggc ggcgctcacc agcaatatcg
     1861 acctgcgttt cgcggacgcc cagcagaagc aggaaaacca gatcaaggcg ctgcgcagtt
     1921 tcatcgcgca gaaggtcgac gtgatcgcgt tctctccggt ggtcgccacg ggctgggaca
     1981 ccgtgctcat ggaagcacgc gccgccggca ttcccgtgat cctcaccgat cgcgcggtga
     2041 ccagcgatcc ttcgctttac gtcggcttcc tgggttcgga cttcgtcgag gaaggccgca
     2101 aggctggccg ctggaccctc gagaagttca aggacatccc gggcgaggtg aacatcgtcg
     2161 agctgcaggg caccgtcggt gcggcgcccg ccaacgatcg caagaaaggt ttcgaagaaa
     2221 tcatcgccgc cgatccgcgc ttccagatca tccgttcgca gaccggggat ttcacgcgca
     2281 ccaagggcaa ggaggcgatg gaggccatcc tcaaatccga gtcgcgcaag atccacctgc
     2341 tgtacgcgca caacgacgac atggccatcg gcgccatcca ggccatcgag gaagcgggcc
     2401 tcaagcccgg caaggaaatc ctcatcgtgt ccatcgacgc ggtgaagggc gcgttcgaag
     2461 ccatgatcgc cggcaagctg aacgccacca tcgagtgcaa tccgttgctg ggcccgcagc
     2521 tcatgacgtc ggtcaccgaa gtggtgtcgg gccgcccgat tccgaaacgc atcgtcgtcg
     2581 aagaaaccat gttcaccatg gaaacggcga aacaacaaat gcatacgcgc aagtactgag
     2641 ctcacgcgtg aatgatccgg cgatcgtatt gcgggcgcac gacatctccc gcgcgtttcc
     2701 cggcgtgcag gcgctgtccg gcgcctcact cacgttgcgc gccggcgaag tccacgcgct
     2761 catgggacag aacggcgccg gcaagtccac gctcatcaag gtactgaccg gcctctgcgc
     2821 gcccgactcc ggcagcatcg aactactggg caaggtcgtt tcgccacagt cgcccgtcca
     2881 tgcgcagcag ctcggcatca gcgcggtgca ccaggaaagc cacctgctgc ccaatctgtc
     2941 cgtggcggag aacatctgcg ccggacgcta cccgcgccat ccctggaccc ggggcggcgg
     3001 catcgactgg cgcgaaaccg agcgccgggc cgcggagttg ttagccagcc tcggcataaa
     3061 gctcgatgtc acgcagctgg ccggcggatt gccggccgcc ctgcagcagc tcgcgaccgt
     3121 cgcgcgcgcc gtcgccaccg atgcacgcgt gctgatcctc gacgaaccga cgtcgagcct
     3181 cgacgccgat gaggtgcagg cgctgttcgc cctcatccgc cggcttcgcg acaagggcgt
     3241 cgcgatcctc ttcgtcacgc atttcctcga ccaggtctac gacatctgcg atcgcatgac
     3301 ggtgctgcgc aatggccggt tcgtcggcga atacgcgtgc gcgaagctcg atgcccacgg
     3361 actggtcgcc gcgatggtcg gccgcgagtt gtcggcgcct gcggctgcgc cggatcatgc
     3421 cgccgcgggc gctgacccac actccgcggc ccctcccgtg ctcgccgcgc gcaacctctc
     3481 gcgccgcggc cagctcaaac cactcgacat cgagcttcat gccggcgaag ttctgggcct
     3541 cgccggcttg ttgggctcgg gccgcaccga actcgcgcgg ctgctgttcg ctctcgatcg
     3601 acgcgacacg ggcgagatca ccatcgacgg caagccggcg gatctcgaca gccccgccga
     3661 cgcgcttcgc gcgggactcg ggttttgccc cgaagaccgc aaggacagcg gcatcgtcgc
     3721 cgacctgtcg gtgcgcgaga acatcgcgct ggcgttgcag gcgcgcatgg gactcacgaa
     3781 attcctcaag gtgagtgacc agcgccagat cgccgagaag atgatcgcag cactcggcat
     3841 caagacagcg agcatcgaaa caccgatcgg tcagctctcg ggtggcaatc agcagaaagc
     3901 gatcatcgcg cgctggctcg ccacccatcc gcgcgtgctg atactcgacg agcccacgcg
     3961 cggcatcgac gtcgccgcca aacaggagct gatgaacgag atcctggaac tcgcccgcgg
     4021 cggcactgcc gtgttgttca tctccgcgga gatagacgaa gtggtgcgcg tttccgatcg
     4081 catcctggtg ctgcgcgacc gcgccagggc cggcgaactg ccgcgtggcg ccaccgaaga
     4141 tgccgtgtat tcactgatcg ccgcgagcca cgaggcatga agcggctcgc gcaacacgcg
     4201 ttgttctggc cggccgtcac gctggcgctg ctgctggtcg ccagcagcct gttcaaccct
     4261 tcgttcctga cgctcaggtg gcatgaagga catctctacg gcagcgtcat cgacatcctc
     4321 aaccgcgccg cgccgctcat cgtggtgtcg ttgggaatga cgctggtgat cgccgtgcgc
     4381 ggactcgata tctcggtggg cgccgtggtg gcgatttccg cggcggtggc cgcactcatg
     4441 atcggcggca gcttcagcgc aaccggcgag gtccatagcg agaatccctt gtggctggcg
     4501 ctggttaccg cgctcggggt cgcggcggcc tgcggcttgt ggaacgggtt gttggtggtg
     4561 aaggccggca tgcagcccat cgtcgccacg ctcatcctca tggtggcggg ccgtggtatc
     4621 gcgcagttgc tcacgggcgg ccagatcatc accgtctact acccgccgtt cttcacgctc
     4681 ggcaagggct tcctgttggg cctgccggtg gcgctgtgga tcgccgcgct gttgtggacg
     4741 tcgctgcatc tcgtgttgtc gcgcacggcg ctgggtctct tcgtgcgctc gatcggcggc
     4801 aatcccgaag cggcgcgcgt cgccggcgtg cgctcctcct ccatcacact gtgcgtgtac
     4861 acattctgcg gcctcgccgc gggcattgcc gggctcatcg tgagctcgaa cgtgaagagc
     4921 gccgacgcca acaacgcggg acaactgctc gaactcgacg ccatcctcgc ggtgacgctc
     4981 ggcggcacgg cgctcacggg tggccggttc acgctcgccg gcacggtgat cggcgcgctg
     5041 atcatccaga cgctgacttc gatgatctat tcgctgggcg tcccgccgga agtgaatctc
     5101 gtggtgaagg ccgcgctggt gttcgcggtg atgctgctgc agtcggcgga attccggcgg
     5161 atggtggccc agctcgcgcg cgggagagcg gcatgaaccg caggctgctg ccgctcacgg
     5221 tcaccatcct cctgttcgtg gcgatggccg cgttcggcgc catccgcttc gacggctttc
     5281 tctcgatgca ggtgttcctc aacctcatca tcgacaacgc cttcctgtgt atcgtcgcgg
     5341 tgggcatgac gttcgtgatc ctgtcgggcg gcatcgatct gtcggtgggc tcgatcatcg
     5401 cactgacgac gatgatcctg gccacgctgc tcaatgccgg ctggaa
//
LOCUS       DATDEU010000394         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_657408, whole genome shotgun sequence.
ACCESSION   DATDEU010000394 DATDEU010000000
VERSION     DATDEU010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657408"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1374)
                     /locus_tag="VIV63_09420"
     CDS             complement(100..1374)
                     /locus_tag="VIV63_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6124860.1"
                     /translation="MSLSIAFLLQMFVILAACRFFGWLVQRWMRQPPVIGEMIAGVVL
                     GPSLLGVVAPELQAFLFPPDSKPVLYVVAQLGIGLYMFLVGLEFRSDEFRSNAPSAVA
                     VSVAGIVVPFIVAIAGTPLLMNVPELFSEGVSRFNATLFMGAAIAITAFPVLARIIQE
                     RGLNGSLIATLSLSAAAIGDAIAWCVLAVVLACLGAGPAVAITAIVGGIILSAVMILL
                     GPRLFAPLGRLAEREQAAGQPLSTTVLAAALMLFSVSAWFADFIGLHAVFGGFLIGTA
                     MPRGVFAERVRKVLEPFTVVFLLPVFFTYSGLNTQLTMVNSISLLLIALGVLAASIFA
                     KFVACWAAARLSGQDSRSALGIGALMNARGLTELIILNIGLQAGIIGPALFSMMVLMA
                     IVTTLMASPLFEAVCGRRARANGELPPLNRVR"
     gene            complement(1376..2908)
                     /locus_tag="VIV63_09425"
     CDS             complement(1376..2908)
                     /locus_tag="VIV63_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6124861.1"
                     /translation="MENAEAATNSTGEGAVRKVVIAGGGTSGWVAAMALSSKFGDLLD
                     ITLVESEEIGTIGVGESTVPPIVTFHRLLGIDEREFMRECAATFKVAIWFENWGRLGD
                     WYLHPFGRHGQPAWMAEFYNFWLHARANGHKVPIGEYCHEWLAAVKGRFALRENPRVN
                     YAYHFDAALYARYLRKLSEKKGVKRVEGKIRNVRLRTDNGFVEALELEDGKVLAGDFF
                     IDCTGFRGVLIEGALKTGYEDWSNYLPCDSAAAFQTIVDEPAPPYTACYAHTAGWRWN
                     IPVQHRVGNGVVFCSRFMSDDEAKHRLVTDSGGRPVKEPWLIRIKAGRRKQAWNKNVV
                     AFGLAAGFVEPLESTSIHTITTAAVRLMTLFPFGGVNQPMTDHYNLLTRNELEHIRDF
                     VCMHYKYNQRDDAPFWKECREMEIPDSLRQRVELFRQGGYAYVKDGELMTVDSWISVM
                     MGQHIQPISYNRFAQVDDRELKDFMNKYRAKVAEVVNGLPLHADFVKQYCPASESAWI
                     KR"
BASE COUNT          577 a          958 c          950 g          490 t
ORIGIN      
        1 ctcaccactg gcgcgggcca gcttgccgta aaccacctcg acgggctttt atacgggcga
       61 tgggccgcgc ccgcccagat ggcgagccat aaaatctccc tatcgaacgc ggttcagcgg
      121 gggcaactcg ccgttcgcgc gagcacgacg gccgcagacc gcttcgaaga gcggggatgc
      181 catgagcgtg gtaacgatgg ccatcaggac catcatggag aacaacgccg ggccgatgat
      241 gccggcctgc aaaccgatgt tgaggatgat cagctccgtg agaccgcgcg cattcatgag
      301 cgcgccgatg ccgagtgcac tgcgggagtc ctggccggac aggcgcgccg ctgcccagca
      361 cgcgacgaac ttcgcgaaga tggaggccgc gagcacgccg agcgcgatca gcaacagcga
      421 gatggaattc accatcgtca gctgcgtgtt gagccccgaa tacgtgaaga acacgggcag
      481 aagaaagacg acggtgaagg gctcgagcac cttcctgacc ctttcggcga acacgcctcg
      541 cggcatggcg gtgccgatca ggaaaccgcc gaagaccgcg tgcagaccga tgaagtccgc
      601 gaaccacgcc gacaccgaga acaacatgag cgccgccgcg agcactgtcg tgctcaacgg
      661 ctggcccgcc gcctgttcgc gctccgccag ccggccgagt ggcgcaaaca ggcgcgggcc
      721 gagaagaatc atcacggcag acagaatgat cccgccgacg atggcggtga tggcgaccgc
      781 cggtcccgca cccaggcagg cgagcacgac ggcgagcacg caccacgcga tggcatcgcc
      841 gatcgccgcg gcggagagcg acaacgtggc tatcaaagaa ccgttgagcc cgcgttcctg
      901 gatgatgcgt gcgagaaccg ggaacgcggt gatggcgatg gcggcgccca tgaacagcgt
      961 cgcgttgaat cgcgagacgc cctcgctgaa aagctccggc acattcatga gaagcggtgt
     1021 gccggcgatg gcgacgatga acggcacgac gatccccgcc acggacacag ccaccgcact
     1081 gggggcgttc gaccggaatt cgtcgctgcg gaattcgagc ccgaccagga acatgtagag
     1141 gccgattccg agctgtgcca ccacgtagag caccggcttc gaatccggcg gaaagaggaa
     1201 tgcctgcaat tccggggcga cgacacctaa caacgatggc ccgagcacca caccggcgat
     1261 catctcgccg atgaccggcg gctggcgcat ccatcgctgt accagccagc cgaagaaccg
     1321 gcatgccgcc aggatgacga acatctgcag caggaacgcg atcgacagac tcacttcagc
     1381 gtttgatcca ggcgctttcg ctcgccgggc agtactgctt gacgaaatcc gcgtgcagcg
     1441 gcaatccgtt gaccacctcg gcgaccttgg cgcggtactt gttcatgaag tccttgagct
     1501 cgcgatcgtc gacctgcgcg aagcggttgt agctgatggg ctggatgtgc tgacccatca
     1561 ttaccgagat ccacgaatcg acggtcatga gctcgccatc cttcacgtag gcgtagccgc
     1621 cctggcggaa cagttccacg cgctggcgca gcgagtcggg gatttccatt tcgcggcact
     1681 ctttccagaa cggcgcgtcg tcgcgctggt tgtatttgta gtgcatgcac acgaagtcgc
     1741 ggatgtgctc gagttcgttg cgcgtgagca ggttgtagtg atcggtcatc ggctggttga
     1801 cgccgccgaa cgggaaaagc gtcatcagcc gcaccgcggc cgtggtgatg gtgtgaatgc
     1861 tggtcgactc gagtggctcg acgaatccgg ccgcgaggcc aaacgccacg acgttcttgt
     1921 tccacgcctg tttgcgccgg ccggccttga tgcggatcag ccacggctcc ttgaccggcc
     1981 gcccgcccga atccgtgacc aggcggtgct tcgcctcgtc gtcggacatg aaccggctgc
     2041 agaacaccac gccgttgccg acgcggtgct gcacgggaat gttccagcgc cagccggcgg
     2101 tgtgcgcgta acacgccgtg tacggcggcg cgggctcgtc gacgatggtc tgaaacgccg
     2161 ccgcgctgtc gcagggaaga tagttggacc agtcttcata gccggtcttc agcgcgccct
     2221 cgatgagcac tccgcgaaaa ccggtgcagt cgatgaagaa atcgccggcc agcaccttgc
     2281 cgtcctcgag ctccagcgct tcgacgaagc cgttgtcggt gcgcaggcga acgttcctga
     2341 tcttgccctc gacgcgcttg acgcccttct tctccgacag cttgcgcagg tatcgcgcgt
     2401 acagggcagc gtcgaagtga tacgcgtagt tgacgcgcgg gttctcgcgt aacgcgaaac
     2461 ggcccttcac ggccgccagc cattcgtggc aatactcgcc gatcggcacc ttgtgtccgt
     2521 tcgcgcgcgc gtgcagccag aagttgtaga actccgccat ccatgcgggt tggccgtggc
     2581 ggccgaaggg atgcaggtac cagtcgccga ggcggcccca gttctcgaac cagatggcga
     2641 ccttgaacgt ggccgcgcac tcgcgcatga attcgcgctc gtcgatgcct aacaaccggt
     2701 ggaacgtgac gatcggcggc acggtggact caccgacgcc gatggtgccg atctcctccg
     2761 attccacgag cgtgatgtcg agcaggtcgc cgaacttcga cgacagcgcc atggctgcga
     2821 cccaacccga ggtgccgccg ccggcaatga cgactttcct gaccgcccct tcccctgtgg
     2881 agttggttgc ggcttccgcg ttttccattc gaaccccctt ctcgaggggg cattatgggg
     2941 tcaggcaccg tttcccagga aacggtgcct ggcac
//
LOCUS       DATDEU010000395         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1823958, whole genome shotgun sequence.
ACCESSION   DATDEU010000395 DATDEU010000000
VERSION     DATDEU010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823958"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VIV63_09430"
     CDS             <1..1068
                     /locus_tag="VIV63_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY6124862.1"
                     /translation="IAHLACRALGKPVHPNDHVNMCQSSNDVIPSAIHLSALTTSVTL
                     HRALGTLRNVLLQKEREWADVLKTGRTHLMDAMPMTLGQEVSGWRAQIDNAVARLQSS
                     EPRLLALAQGGTAIGTGINAHEKFAATFIDELRAATGLALTLAPNYFAAMGSQDAAVE
                     YSGQLRTLAVALMKISNDLRWMNSGPLAGLAEIKLPALQPGSSIMPGKVNPIVPEAVA
                     MVAAQVMGYDAAIAIAGQSGNFQLNVMLPLIAKNLIESILLLASAASALANQVLPKTQ
                     ANVERLNEALARNPILVTALNPVIGYERAAMIAKRAYAEGRAVFDVALEMSGLKEDEL
                     RKLLDPAALTRGGLHGGGGSG"
     gene            1079..1717
                     /locus_tag="VIV63_09435"
     CDS             1079..1717
                     /locus_tag="VIV63_09435"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY6124863.1"
                     /translation="MKEQEQLRSRILRRFEGSQPRHELADWRLLGVDAERSKRLQRHF
                     PANPVAAAVLVPLVDRPEGLTVLLTQRASQMTRHAAQISFPGGRLDPTDADVTSAALR
                     EAREEIGLDPAHVNVFGYLPDHLVISGYRVTPVLSLVTPPFSLTLNPAEVAEVFEVPI
                     SHVFDPDNHKARLRRVGDEDMLLYDIPWQGQNIWGATAGMLLTFVRMVQEGA"
     gene            1714..>2165
                     /locus_tag="VIV63_09440"
     CDS             1714..>2165
                     /locus_tag="VIV63_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEY6124864.1"
                     /translation="MSDEADLKAQRSLSRLLGVMRRLRSPDGCPWDREQDFASIAPYT
                     IEEAYEVAEAIARGEPRALEGELGDLLFQVVFHAQMGAERQWFDFASVAESIADKLTE
                     RHPHVFADARIESVADQNRAWEEHKARLRRVGDEDMLLYDIPWQGQNI"
BASE COUNT          392 a          719 c          714 g          340 t
ORIGIN      
        1 atcgctcacc tcgcgtgccg ggcgctcggc aaacccgtgc atccgaacga tcacgtcaac
       61 atgtgccaga gcagcaacga cgtgatcccg agtgcgatcc acctgagcgc gctgaccacg
      121 agcgtcacgc ttcaccgggc cctcggcact cttcgcaacg tgctgctgca gaaagagcgt
      181 gaatgggccg atgtcctcaa gaccggccgc acccatctca tggacgcgat gccgatgacg
      241 ctcggccagg aagtctccgg ctggcgcgcg cagatcgaca atgccgtcgc gcgcctccag
      301 tcgagcgagc ctcgattgct ggcactggcg cagggtggca cggccatcgg caccggcatc
      361 aacgcgcatg agaaattcgc ggcgacgttc atcgatgaac tccgcgccgc gaccggcctt
      421 gcgctcacgc tcgcacccaa ctacttcgcg gccatggggt cgcaggacgc ggcggtcgaa
      481 tattcggggc aactgcgcac gctcgcagtc gcgctcatga agatcagcaa cgatctgcgc
      541 tggatgaaca gcgggccgct cgcggggctt gccgagatca agctgccggc gcttcagccc
      601 ggcagcagca tcatgccggg caaggtcaac cccatcgtgc ccgaagcggt ggcgatggtt
      661 gcggcgcagg tcatgggata cgacgcggcg atcgcgatcg cgggccagtc cggcaatttc
      721 cagctcaacg tgatgctgcc gctgatcgcg aagaatctga tcgaatccat cctgctcctc
      781 gcgagcgccg cgtcggcact ggcgaaccag gtgctgccca aaacgcaggc aaacgtcgaa
      841 cggctcaacg aggcgctggc gcgcaatccc atcctcgtca cggcgctgaa tcccgtcatc
      901 ggttacgaac gcgcggccat gatcgccaag cgcgcgtacg ccgagggtcg tgcggtcttc
      961 gacgtggcac tcgagatgtc gggactgaag gaagacgagt tgcgcaagct gctcgatccg
     1021 gcggcgctca cgcgaggcgg cctgcatggc ggcggaggct ctggttgaag gagggcggtt
     1081 gaaggagcag gagcaactgc gcagccgcat cctgcggcgc ttcgaaggct cgcaaccccg
     1141 ccacgagctt gccgactggc gattgctggg agtggacgcg gaacgaagca agcgtttgca
     1201 acggcacttt cccgccaacc cggttgcggc cgcggtcctc gtgccgctcg tggaccggcc
     1261 ggaagggttg acggtgctgc tcacgcaacg cgcctcgcag atgacgcggc atgcggcgca
     1321 gatttccttc cccggcggcc gcctcgatcc gaccgacgcc gacgtcacca gtgccgcgct
     1381 gcgcgaagcg cgcgaagaaa tcggcctcga tccggcgcat gtcaacgtgt tcggctatct
     1441 accggatcac ctcgtcatca gcggataccg tgtgacgccg gtgttgtcgc tggtcacgcc
     1501 gccgttctcg ctcacgctca accccgcgga ggtcgccgaa gtgttcgagg tccccatcag
     1561 ccacgtgttc gaccccgata atcacaaggc gcggctgcgg cgcgtcggcg atgaagacat
     1621 gttgctgtac gacattccgt ggcagggcca gaacatctgg ggcgcgacgg cgggcatgct
     1681 gctcaccttc gtgcgcatgg tgcaggaggg cgcgtgagcg acgaagcgga tctgaaagcg
     1741 caacgttcgc tgtcgcggct gctcggcgtc atgcgacgtc tgcgctcccc cgacggctgc
     1801 ccctgggacc gcgaacagga cttcgcgagc atcgcgccgt acaccatcga agaggcctac
     1861 gaggtcgccg aagccatcgc acgcggcgag ccgcgcgcgc tcgaaggcga actgggcgac
     1921 ctgctgttcc aggtggtgtt ccacgcgcag atgggcgccg agcgccagtg gttcgatttc
     1981 gcgagcgtcg ccgaatccat cgccgacaag ctcaccgaac gccaccccca tgtgttcgcg
     2041 gatgcgcgca tcgagtcggt ggccgaccag aaccgcgcct gggaggaaca caaggcgcgg
     2101 ctgcggcgcg tcggcgatga agacatgttg ctgtacgaca ttccgtggca gggccagaac
     2161 atctg
//
LOCUS       DATDEU010000396         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_73511, whole genome shotgun sequence.
ACCESSION   DATDEU010000396 DATDEU010000000
VERSION     DATDEU010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73511"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1217)
                     /locus_tag="VIV63_09445"
     CDS             complement(90..1217)
                     /locus_tag="VIV63_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY6124865.1"
                     /translation="MPITIGALRESAPGETRVSLVPEVVDKFLAAGARVVLEPGAGIR
                     AQFPDALYKKGEWLASSAAVLSQADVLLTVAPLSLEQISQLKPGAVVVGFLQAHTKHA
                     EVKALRDRKITSFAVELIPRISRAQSMDGLSSQASLAGYKAVLIAANTLEKFMPMLTT
                     AAGTIRPSQALIIGAGVAGLQAIATARRLGAVVEAYDVRSATREQVKSLGAKFVETGV
                     SADGVGGYARELTTEEKARQQEALDARIAAADAVISTASVPGRKAPVIISKAAVERMR
                     AGSVIVDIAAEQGGNCELTRAGETVIHQGVKILGPVNVAGTLAYNASEMYARNLLNFL
                     KPAIDKAGDLKIDWADEVFAGAVLTHDGAIKHEATRKAVEG"
     gene            1462..2019
                     /locus_tag="VIV63_09450"
     CDS             1462..2019
                     /locus_tag="VIV63_09450"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY6124866.1"
                     /translation="MPLEWIDYKEAARLYHQEQVAYTCGSPLFKLYGGVSARTGFRTV
                     LEVNSIVATVGHTGNPGNTRHDYVPPLNNQTLFRRDAHFCLYCAQRFPVRELSRDHIR
                     PFSQGGRDVWTNVVAACRRCNNLKASRTPEQAKMQLIAVPFTPTYAEYIFLKGRRVLA
                     DQMEYLRAHFPRHSPLHARIKRYLS"
BASE COUNT          353 a          671 c          669 g          402 t
ORIGIN      
        1 caggatcacc ggcaccttgg cgatgatttc gtagccggtg aaagccgcca gcatgaagat
       61 gtagagagca acgattccag tcattgtgat tagccttcca ctgccttgcg cgtggcttcg
      121 tgtttgatgg cgccgtcgtg cgtgagcacg gcgccggcga atacttcgtc cgcccagtcg
      181 attttcagat cgccggcctt gtcgatggcg ggcttcagga aattgagcag gttgcgcgcg
      241 tacatttcgc tggcgttgta ggcgagggtg cccgccacgt tgacgggccc caggatcttc
      301 acgccctggt ggatgaccgt ttcgccggcg cgggtgagtt cgcaattgcc gccctgttcg
      361 gccgcgatgt cgacgatgac cgagccggcg cgcatacgtt cgacggcggc cttcgagatg
      421 atgaccggcg ccttccggcc cggaacggac gcggtggaaa tgacagcatc ggcggcggca
      481 attcgcgcat ccagcgcttc ctgctgcctg gccttttcct cggttgtgag ctcgcgggcg
      541 taaccgccaa cgccatcggc gctgaccccg gtctccacga acttggcgcc cagcgacttc
      601 acctgttcac gggtggcact tcgcacgtca tacgcttcga cgaccgcgcc cagacggcga
      661 gcggtggcga tggcctgcag acccgcgacc ccggcgccga tgatcagcgc ctgggacggg
      721 cggatcgttc cggccgccgt cgtcagcatg ggcatgaact tttcgagcgt attggcggcg
      781 atcaggaccg ccttgtagcc ggccagagag gcctgggaag acaggccgtc catggactgg
      841 gcccgcgaaa tacgcggaat gagctcgacg gcgaagctgg tgatcttgcg atcgcgcagc
      901 gccttcacct cggcgtgttt ggtgtgggcc tgcaggaaac cgaccaccac ggcgcccggt
      961 ttgagctggg atatctgctc caggctcagc ggggccacgg tgagcaggac gtcggcctgt
     1021 gacagcaccg cggccgaact agctaaccat tcacccttct tgtagagcgc atccggaaac
     1081 tgggcgcgaa tcccggcccc gggctcgagc accacgcgtg ctccggcggc caggaatttg
     1141 tcgacgacct cgggtacgag gctgacgcgc gtttcaccgg gcgcgctctc gcgcagcgcg
     1201 cctatcgtaa tcggcatttg gctttgactc ccctgatcgg caagaggcct gtggcagtcc
     1261 cgccaaaggc ctttttttcc cgtgttaaat gaatgtcttg cggcgttgaa ttctcgcaca
     1321 cgactggtct atcttacatg cctcatgttg ggcatcgatc ttgaaaaggc agatcgaggt
     1381 gacggctcgg atctgcgcct gcttgcgagc gacttgggtc gcgcgctctc ccaactcgtc
     1441 ctgcgaacgg acgtgtcggg aatgccgctc gaatggatcg actacaagga agcggcccgt
     1501 ctctatcacc aggagcaggt cgcctatacc tgcggcagcc cgctgttcaa gttgtacggc
     1561 ggtgttagcg ctcgcacggg tttccggacc gtgctggagg tcaactccat cgtcgcgacg
     1621 gtcggccaca ccggcaatcc cggcaacacc cgccatgatt acgtgccgcc gctgaacaac
     1681 cagacgttgt tccggcgcga tgcgcacttc tgcctgtatt gcgcacagag gttccccgtg
     1741 cgcgagctgt cacgcgatca catccggccg ttctcgcagg gtggtcgtga cgtgtggacg
     1801 aatgtggtcg cggcctgccg ccgctgcaac aacctcaagg cgtcgcgtac gcccgagcag
     1861 gccaagatgc agctcatcgc ggtgccgttc acgccgacct acgcggagta catcttcctg
     1921 aagggccggc gggtgctcgc cgaccagatg gaatatctgc gcgcgcactt cccgcggcac
     1981 tcgccgctgc acgcgcgcat caagcgctac ctgagctgaa gacggagcta gctactgcgc
     2041 ggtgacgcgc agccagaatc gcgcgaccac ttcctcgtgg tcgggcgaat gcccg
//
LOCUS       DATDEU010000397         2096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_73556, whole genome shotgun sequence.
ACCESSION   DATDEU010000397 DATDEU010000000
VERSION     DATDEU010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73556"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VIV63_09455"
     CDS             <1..928
                     /locus_tag="VIV63_09455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170938.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY6124867.1"
                     /translation="DVPEENHRIARDIGYPVIIKASGGGGGRGMRVVHTEASLLNAIA
                     VTKAEALAAFNNDQVYMEKFLERPRHIEFQVLADNYGNVIHLGERDCSMQRRHQKVVE
                     EAPAPGITEAQREKMGARVVEACKAVGYRSAGTLEFLYQDDEFYFIEMNTRIQVEHPV
                     TELITGIDLVKAQLLIASGEKLQYSQNDIKLTGHAIECRINAEDAKTFMPSPGPIKLW
                     HAPGGPGIRVDSHIYSGYNVPPHYDSLIAKVISYGDTRETAIARMRNALAEMVVEGIK
                     TNTALHQEIFTHQAFRTGGQDIHYLERRLGLK"
     gene            943..1821
                     /gene="prmA"
                     /locus_tag="VIV63_09460"
     CDS             943..1821
                     /gene="prmA"
                     /locus_tag="VIV63_09460"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY6124868.1"
                     /translation="MPHLALTLTLDGLDAERVEEACLEFGAVAVSYTDQRDDPILEPA
                     PGEFRLWPHSRLQALFPFDTSPQELIAGLSHVLRVEPARLTLETLADRVWEREWLRDF
                     HPMCFGRRLWVAPHHAHVHTKDAVIVRLDPGLAFGTGTHATTAMCLAWLDENAAEGQT
                     AIDYGCGSGVLAVAAVKLGAREAHAFDIDPQALTATHDNANANGVEAKVRVVDTDAAL
                     PANADILLANILCGPLCDLAPRFAALTRPGGKIVLAGLLTAQADEVTRAHAPWFDIAP
                     FASRDGWTALTGSRRA"
     gene            1846..>2096
                     /locus_tag="VIV63_09465"
     CDS             1846..>2096
                     /locus_tag="VIV63_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEY6124869.1"
                     /translation="MFTVCPKCTLTLVVTTVDLRAGQGYVRCGRCANVFNALIALREG
                     EPGSGASDTAKRRLLETKPDQTIEPALGSGVALDLEPEP"
BASE COUNT          387 a          699 c          666 g          344 t
ORIGIN      
        1 cgacgtgccc gaagagaacc atcgcatcgc acgcgacatc ggctatccgg tgatcatcaa
       61 ggcttcgggc ggcggcggcg gccgtggcat gcgcgtggtg cacaccgagg cctcgttgct
      121 gaatgccatc gcggtcacca aggcggaagc cttggcggcc ttcaacaacg accaggtcta
      181 catggagaaa ttcctggagc gcccgcgtca catcgaattc caggtattgg ccgacaacta
      241 cggcaacgtg attcacctcg gcgaacgcga ctgctccatg cagcgccgcc atcagaaggt
      301 ggtcgaggaa gcgcccgcgc ccggaatcac ggaagcgcag cgcgagaaga tgggcgcgcg
      361 cgtggtcgag gcctgcaagg ccgtgggcta tcgcagcgcc ggcacgctcg agttcctcta
      421 ccaggacgac gagttctact tcatcgagat gaacacgcgc atccaggtgg aacacccggt
      481 gaccgagctc atcacgggta tagacctggt gaaggcgcag ctgttgatcg cgtccggcga
      541 gaagctgcag tactcgcaga atgacatcaa gctcaccggc catgcgatcg aatgccgcat
      601 caacgccgag gacgccaaga ccttcatgcc ctcgccgggc ccgatcaagc tctggcacgc
      661 gccgggcggc ccgggtatcc gggtagatag tcacatctac tcgggctaca acgtgccgcc
      721 gcattacgac tcgctgatcg ccaaggtcat ttcctatggc gacacgcggg aaaccgcgat
      781 cgcccgcatg cgcaatgcgc tcgccgaaat ggttgtcgaa ggcatcaaga ccaacacggc
      841 gttgcaccag gaaatcttca cgcaccaggc tttccgcacg ggcggccagg acattcacta
      901 cctggagcgc cgcctcggcc tcaagtagca ggtagtccac gcgtgccgca ccttgccctc
      961 acgctgactc tggatggact cgacgcggaa cgtgtcgaag aagcctgtct ggagttcggc
     1021 gccgtcgctg tctcgtatac cgatcagcgc gacgacccga ttctcgaacc ggccccgggt
     1081 gaattccggc tgtggccgca ttcgcggctg caggcgctgt ttccgttcga tacctcgccg
     1141 caggaactga tcgccggcct ttcgcacgtg cttcgcgtcg aacctgcgcg cctcacgctc
     1201 gagacgctgg ccgatcgcgt atgggagcgc gagtggctgc gcgacttcca tcccatgtgc
     1261 ttcgggcggc ggctgtgggt cgcgccccac cacgcgcacg tacacacgaa agacgcggtc
     1321 atcgtgcgcc tggaccccgg gctcgccttc ggcaccggca cccacgccac gacggccatg
     1381 tgcctcgcgt ggctcgacga gaacgcggcg gagggtcaga cggccatcga ctacggctgc
     1441 ggttcgggag tgctggcggt cgccgccgtg aagctcggcg cgcgcgaagc ccacgctttc
     1501 gacatcgatc cgcaggcgtt gacggcgacc cacgacaatg cgaacgccaa cggcgtggaa
     1561 gcaaaagtgc gcgtggtcga tacggacgcc gcgctgcccg ccaacgccga catcctgctg
     1621 gctaacatcc tgtgtggtcc gctgtgcgac ctggctccgc ggtttgccgc gctgacgcgg
     1681 ccgggcggga aaatcgtgct cgcaggcctc ctgaccgcgc aggcggacga ggtgacacgc
     1741 gcgcacgcac cgtggtttga tattgcgccc tttgcgtccc gcgacggctg gacggcactc
     1801 accggcagtc gtcgcgctta agacgcgcct cgaggagtat ttcgcttgtt caccgtctgc
     1861 ccgaaatgca cgctgaccct ggtcgttacg accgtggatc tgcgcgccgg gcagggatac
     1921 gtgcgctgcg gacgctgcgc gaacgtattc aatgcgctca tcgcattgcg cgagggtgag
     1981 ccgggcagcg gagcttccga caccgccaag cgccggctgc tcgaaaccaa gcctgatcaa
     2041 acaatcgaac ccgcgctcgg atccggcgtt gcgttggatc tggaaccgga acccga
//
LOCUS       DATDEU010000398         7779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_364224, whole genome shotgun sequence.
ACCESSION   DATDEU010000398 DATDEU010000000
VERSION     DATDEU010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364224"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1119
                     /locus_tag="VIV63_09470"
     CDS             328..1119
                     /locus_tag="VIV63_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124870.1"
                     /translation="MSTRLLNGLCLLLLTTFSQFVAASNACSLAPREPGTPIEAPHAV
                     FDAYVIEVDAPRSSPTAFGIRHTRRALVEVVRSFHGPYAPGQQVETLTIERANGCGGV
                     VESGAHVVVRSETGGPFEIIETLSAGSAIPEGPFAALAFAGDEQRKSLRPALKNASLR
                     GGIDFGLAARLHSRARPGREVHSEISGAGNFAQVSWGKAFGGNDARHKVVFERTARGW
                     VEILRYEAPTPTPVRNRGRRAKQRALWAEESFLRRPDAAWAATTS"
     gene            complement(1079..2155)
                     /locus_tag="VIV63_09475"
     CDS             complement(1079..2155)
                     /locus_tag="VIV63_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY6124871.1"
                     /translation="MADAREVSVELLRTLTPLEGMKRENLHALAKKVTVKQMTAGRVL
                     FKEGDADKRTIWIIKGLVELSEQGRTTGMIEGGSADARAPLNQSVPRKVTARAVEDVD
                     YLAIDADLLDVMITWDQTGNYEVGELQAQFGGGNEDSDWMTTLLQTKAFHRIPPANLQ
                     AIFMRMQRTPYRAGEVVIKQGDEGDFFYAIVQGRCLVTRESPLNQKGIKLAELGVGET
                     FGEEALIAEAKRNATVSMLTEGVLMRLNKQDFRDLMHEPLLRWVDEQQALKIIQNRGQ
                     WLDVRLPSEYQNLAIEGSINIPLYFVRLKLNALDRKMPYVVVCDTGRRSSAAAFILSE
                     RGFDAYVLKGGITTSSLPMRRPGA"
     gene            complement(2187..4232)
                     /locus_tag="VIV63_09480"
     CDS             complement(2187..4232)
                     /locus_tag="VIV63_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY6124872.1"
                     /translation="MTTSATGAWALERDADGIAWLSFDKPGSSTNVLSRDVLVELDTH
                     LKTFDSAPPRGVVIRSAKKSGFIAGADVREFTQLTSADQAFELVRAAQGILDHLESLP
                     CPTVAIIHGFALGGGLELALACRYRVGVRGDNFSIGLPEVMLGIHPGFGGTVRAVRRA
                     GVRTAMEMMLTGKTLRADKARNAGFLDRLVYPADAEAAARELINRQPKQRQPRLLDRL
                     LSWPLVRGLVKKQLTAQVRAKARPEHYPAPYAIIDLWSTHGARGEAAYEAEARSIARL
                     MISDTSRNLVRVFLLQDRLKNLGGKAKVPLARVHVVGAGVMGGDIAAWCAQRGLNVTL
                     QDRELKFIEPALQRAREGFEKRLKDKDKAAVLMTRVTADVAGDGVPGADVIIEAIFEN
                     ADAKHELFARLEPRMKASAILASNTSSIMLEQLDDRLPDPTRLVGLHFFNPVAQMPLV
                     EIVRGEQSSEESIQKAIAFTRKIDKLPLPCKSAPGFLVNRVLVPYLYEAMFALQDGIP
                     IEVIDEAALRYGMPMGPVELADVVGLDVCKHVGDIVSKALNKQQPDTSRIEALIAAKK
                     LGRKTGEGFYVWRDGKAVKSKGPHAEPPADLEDRLVLALANECAAVLREGIVADSDLI
                     DAGVIFGSGFAPFRGGPLTYARARGIDVVVARLQELTQAYGSRFTPDKGWTSLRSVP"
     gene            complement(4243..5007)
                     /locus_tag="VIV63_09485"
     CDS             complement(4243..5007)
                     /locus_tag="VIV63_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY6124873.1"
                     /translation="MRLLVYNIRYATGIGPAFHLPFPGAGYLRSSRKVLDGITGFVKA
                     QDPDIVGLIEVDIGSVRSGMVNQAEHIANALGHYTTYECKYGKSSINNLMPIVRKQGN
                     AFLAAPRVEGERFHYFDTGIKRLIIELELDDVCIFLVHLALKYRQRQYQLRYLHDLII
                     KSHKPVIVTGDFNTFWGTHEIYLFMRAAGLRSANEKNVPSFPARIPRIELDFVLVSKE
                     IEVTHFEVPDVRWSDHRPVLCDFNVRGAVASQPAVA"
     gene            5103..5558
                     /locus_tag="VIV63_09490"
     CDS             5103..5558
                     /locus_tag="VIV63_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124874.1"
                     /translation="MSQNNPIRLFVTHAWDTSDEYLRIFEYLESARNFFYKNQSAPDK
                     APPGGGKEADREELRRQIAQAECVIALPGMYRQQSELMLFQLTFAKASDKPVILMRPF
                     GANQVLSKEITSLSDQIVEWDGRALVDAIKAQARHEESNRWDTIEFKLD"
     gene            complement(5559..5921)
                     /locus_tag="VIV63_09495"
     CDS             complement(5559..5921)
                     /locus_tag="VIV63_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124875.1"
                     /translation="MSRVYDRLESATLLLARAGAIKDRLNGAWRQCLFNIEPDDVPRE
                     LRLQFIELASEMQRERPLRGEDAVRATIRKMSNEEAERHAAFIVRMFCRMTRQQELEL
                     PIPVQTSAAVVQLFAAEG"
     gene            complement(6055..>7779)
                     /locus_tag="VIV63_09500"
     CDS             complement(6055..>7779)
                     /locus_tag="VIV63_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY6124876.1"
                     /translation="RAEPAVAYVTLDSRRFAHATNPNDTLFVNQWYLKDAEPAAVNAI
                     GAWDREVGSSGVVIAVLDTGVLYDHPDLGRGDRGGKLLPGFDFVSLQHMSNDADGRDA
                     NPSDPGDWVNSSDKTDAAFTNCDTTGSSWHGTRVAGMIGALTNNSSGVAGLGWNSFIL
                     PVRVLGKCGGIDSDVLAGMRWAAGLHVPGVPDNPTPARILNASLGSGGACEDSYREVI
                     EELTARKVLLVVSAGNEGTMVSSPGNCPGVAGVAAIRHAGSKVGFSSLGPEVTIAAPG
                     GNCVNINGGPCLFSLDTTSNSGTTVPASHIFTDQTNTNLGTSFSAPIVSGIAALMLSR
                     NANLSTTQMLARLREGARPFPTTVTGEPDIPTCHVPVDDDDFQLAQCLCTTSTCGAGM
                     ADAAGSVAAAERPIAAVTLPVQVSAGQNVTLNASASAAACGRTVASYAWSVIDPTIDP
                     PAIGGDGTAVATVIAPSAGTVTVRLTVTDNLGRIDAADVVVGPDNVTTDAPASAGVIA
                     CAVPVTSGPTPVVTPAPSPTPTPPRGSGSRGGGGGSLDLSTVLLLGALCFGVARRRRA
                     VRISRCNC"
BASE COUNT         1399 a         2406 c         2535 g         1439 t
ORIGIN      
        1 ctccgaccag gaccgcggac agacgcagaa gaaaatcgag acctacctgg ccggcgcgct
       61 cgccggcgat aaagccacgc acaccatgta gcgtccggtg cccgcgggca cggcgggaac
      121 gtggttcaca cttgcagttc gggcgtctcc cttttcctta gggctggccc atcgtcgcgc
      181 tcttttcgtg accgcgctcg caaacggtca gcccgattct gataattcga tgcccccggg
      241 atgtgaatga gccatgggac gatcggtctc atgcgtatca ggtcaccacg cgaaaagaga
      301 ccgtattccg ctgatgcaga tcgccgcatg tcgacgcgat tgctcaacgg cctctgcctg
      361 ttgctcctca ctaccttttc gcagttcgtc gcggcctcga acgcgtgcag cttggcgcca
      421 cgagagcccg gcactcccat cgaagcgccc catgccgtct tcgacgccta tgtcatcgaa
      481 gtcgacgcac cgcgtagttc gcccaccgca ttcggaattc ggcacacgcg ccgtgcgctc
      541 gtcgaagtag ttcgctcgtt tcacgggccg tatgctccgg gccagcaggt cgagacactg
      601 acgatcgaac gcgcgaatgg ctgcggcggt gtggtcgagt cgggcgcgca tgtggtggtt
      661 cgctcggaaa ccggcggccc gttcgaaatc atcgagacgc tgtcggcggg atcagccatt
      721 ccggaaggac cgtttgccgc gctcgctttc gccggtgatg agcagcgcaa gagtctgcgc
      781 ccggcgctga agaatgcatc gctgcgcggt ggtatcgact tcggccttgc cgcaaggctc
      841 cattcacgcg cgcggccggg acgggaagtg cacagcgaaa tcagcggcgc cggaaacttt
      901 gcacaggtca gctggggcaa agctttcggt ggcaacgatg cgcggcacaa agtggttttc
      961 gagcgcacgg ctcgcggctg ggtcgagatc ctgcgctatg aggcgcctac acccacgccg
     1021 gtccgaaatc gcggacgccg cgcgaagcag cgtgcgttgt gggccgaaga atcgtttctc
     1081 aggcgcccgg acgccgcatg ggcagcgacg acgtcgtgat gccgcctttc agaacgtagg
     1141 catcgaatcc ccgctccgag agaatgaacg ccgcggctga actgcgccgc ccggtgtcgc
     1201 acaccaccac gtaaggcatc ttgcgatcga gcgcgttcaa cttgagccgc acgaaataca
     1261 gcgggatgtt gatggagcct tcgatggcga ggttctgata ttccgacggc aggcgcacgt
     1321 cgagccactg gccgcggttc tggatgatct tgagcgcctg ttgctcgtcg acccagcgga
     1381 gcaggggctc gtgcatgagg tcgcggaagt cctgcttgtt gaggcgcatg agcacgccct
     1441 cggtcagcat gctgacggtc gcgttgcgct tcgcttcggc gatgagcgct tcctcgccga
     1501 aggtttcgcc gacaccgagt tcggcgagct tgatgccttt ctgattgagc ggcgactcgc
     1561 gtgtgacgag gcagcggccc tggacgatgg cgtagaagaa gtcgccttcg tcgccctgct
     1621 tgatgacgac ttcgccggcg cgatagggcg tgcgctgcat gcgcatgaag atggcctgca
     1681 ggttggccgg cggaatgcgg tggaatgcct tggtctggag cagcgtggtc atccagtcgc
     1741 tgtcttcgtt gccgccgccg aactgcgcct gcagctcgcc gacttcgtaa ttgccggttt
     1801 gatcccaggt gatcatgacg tcgagcaggt cggcatcgat ggcgaggtaa tcgacatctt
     1861 cgacggcgcg agcggtgact ttgcgcggca cgctctggtt gagcggcgcg cgggcatccg
     1921 cgctgccgcc ttcgatcatg ccggtggtgc ggccctgctc ggacaactcg accaggccct
     1981 tgatgatcca gatcgtgcgt ttgtccgcat caccttcctt gaacaacacg cggccagccg
     2041 tcatctgttt gacggtgacc ttcttggcga gcgcgtgcag attttcccgc ttcattcctt
     2101 cgagcggggt caatgttctc agcagttcaa cgctgacttc acgggcatcg gccatgcggg
     2161 aaatgctagc acgccagcga ggcggcttaa ggcacggagc gcaaggaagt ccatcccttg
     2221 tcgggtgtga agcgtgatcc gtacgcttgg gtgagttcct gaagcctcgc gaccacgacg
     2281 tcgatgccac gcgcgcgcgc ataggtgaga gggccgccgc ggaacggcgc aaaacccgaa
     2341 ccgaagatca cgcccgcgtc gatcagatcc gaatcggcga cgattccctc gcgcaatacg
     2401 gccgcgcact cgttggcgag cgcgagcacc agtcggtctt cgagatccgc gggcggctcg
     2461 gcatgcgggc ccttcgactt gacggccttg ccgtcgcgcc agacgtaaaa accttcaccc
     2521 gtcttgcgcc cgagtttctt cgcggcgatc aatgcctcga tgcgcgaggt atcgggctgc
     2581 tgcttgttca gcgccttcga gacgatgtca cccacatgct tgcagacatc gagcccgacc
     2641 acgtcggcga gttccaccgg acccatcggc atgccgtagc gcagcgccgc ttcgtcgatg
     2701 acttcgatgg gaatcccgtc ctgcagggcg aacatcgcct cgtacaggta gggcacgagc
     2761 acgcgattga ccaggaagcc gggcgcgctc ttgcagggca gcggaagttt gtcgatcttg
     2821 cgcgtgaagg cgatggcctt ctggatggac tcttcgctgc tctgctcgcc gcgcacgatt
     2881 tccaccagcg gcatctgcgc caccgggttg aagaagtgca ggcccacgag ccgcgtcgga
     2941 tccgggagcc ggtcgtcgag ctgctcgagc atgatcgacg aagtgttcga cgcgaggatg
     3001 gcgctcgcct tcatacgtgg ttcgagccgc gcgaacagtt cgtgtttcgc atcggcattc
     3061 tcgaaaatcg cttcgatgat cacgtccgcg cccggcacgc catcgccggc gacatccgcc
     3121 gtgacgcgcg tcatcaaaac cgccgccttg tctttgtcct tgagccgctt ttcgaatcct
     3181 tcgcgcgcgc gttgcagtgc gggttcgatg aacttgagct cgcgatcctg gagcgtcacg
     3241 ttcaggccgc gctgtgcgca ccacgcggcg atgtcgccac ccatgacgcc ggcgcccacc
     3301 acgtgaacgc gcgcgagcgg caccttggct ttgccaccga gattcttgag ccggtcctgc
     3361 aacaggaaaa cccgtacgag attgcgcgag gtgtcactga tcatgagccg cgcgatggag
     3421 cgcgcctcgg cctcgtatgc ggcctcgcca cgggcgccgt gcgtcgacca caggtcgatg
     3481 atggcgtagg gagcagggta gtgctcgggg cgcgccttgg cgcgcacctg ggctgtgagc
     3541 tgcttcttca cgagcccgcg aaccagtggc cacgacagga ggcgatccag cagccgcggc
     3601 tgacgttgtt tgggctgccg attgatgagc tcgcgcgccg cggcttcggc gtccgccggg
     3661 tacaccagcc ggtcgagaaa gcctgcgtta cgggccttgt cggcccgcag ggtcttgccc
     3721 gtgagcatca tctccatcgc cgttcggact cccgcgcgac gcaccgcgcg cacggttccg
     3781 ccaaatccgg gatgaatgcc cagcatgact tccggcagac cgatcgagaa attgtcgccg
     3841 cggactccga cgcgatagcg gcaggcgagc gccagttcga gtccgccgcc cagcgcgaag
     3901 ccgtggatga tggccaccgt cgggcagggc aatgattcca ggtgatcgag gatgccctgc
     3961 gcggcgcgca ccagttcgaa agcctggtcg gcactggtga gctgcgtgaa ctcgcgcacg
     4021 tcggcgccag cgatgaatcc ggatttcttc gcggagcgga tgaccactcc gcgcggcggc
     4081 gcgctgtcga atgtcttgag atgggtatcg agctcgacga gcacgtcacg cgacagcacg
     4141 ttggtggagc tgccgggctt gtcgaagctg agccaggcaa tgccgtcggc atcgcgctcg
     4201 agcgcccagg cgccggtggc ggaagttgtc atgaacgcat tttcaggcga cggcgggctg
     4261 cgaagctact gcgccgcgaa cattgaagtc gcagagcacc ggccgatgat cggaccagcg
     4321 cacatccggg acttcgaagt gcgtgacctc gatttccttg ctgacgagca cgaagtcgag
     4381 ctcgatgcgg gggatacgcg ccggaaacga aggaacgttc ttttcattgg cgctgcgcaa
     4441 gccggcggcg cgcatgaaca ggtagatttc gtgcgttccc cagaaggtat tgaaatcgcc
     4501 ggtcacgatg accggtttgt gcgacttgat gatgaggtcg tgcaggtacc gcagctggta
     4561 ctggcgctgg cgatacttca gggcaaggtg gacgaggaag atgcacacgt cgtcaagctc
     4621 gagctcgatg atgagccgct tgatgccggt gtcgaagtag tggaagcgtt cgccctcgac
     4681 gcgcggcgcc gccaggaacg cgttgccctg cttgcgaacg atgggcatga ggttgttgat
     4741 cgagctcttg ccgtacttgc actcgtacgt cgtgtagtgc ccgagcgcgt tggcgatgtg
     4801 ttcggcctgg ttgaccatgc cagagcgtac cgagccgatg tcgacctcga tgagcccgac
     4861 gatgtccggg tcctgcgcct tcacgaagcc cgttatgccg tcgagtacct tgcggctgga
     4921 tcgtagatag ccggctcccg ggaacggcaa atggaatgct ggtccaatgc ccgtggcgta
     4981 gcgaatgttg tagacgagca ggcgcaagcg gattgatgct aacggtgcgc cgcacaaaag
     5041 gggagtgggg cgtaacttag gcgtctaaac tacactcaag cccagggttt ggatcggtcg
     5101 gcatgtcaca aaacaatcca attagactgt tcgtcacgca tgcgtgggac acttccgacg
     5161 aatatctccg tatattcgag tatctcgaga gtgcgcggaa cttcttctac aagaaccagt
     5221 cggcgcctga caaggcgcca ccgggcggtg gcaaggaagc ggatcgcgag gaactgcggc
     5281 gccagatcgc ccaggcggaa tgtgtcatcg cgttgcctgg aatgtatcgc cagcagagcg
     5341 aactcatgct tttccagctc accttcgcga aagcctcgga caagccggtc attctcatgc
     5401 ggccgttcgg cgccaaccag gtgctctcga aagagatcac gtctttgtcg gaccagatcg
     5461 tggaatggga cggtcgcgcg ctggtcgatg ccatcaaggc gcaggcccgc cacgaagaat
     5521 cgaatcgatg ggacaccatc gaattcaagc tcgactaact atccttcggc agcgaacagc
     5581 tgcaccacgg cggcgctcgt ctgcacggga atcggcagtt cgagttcctg ctggcgcgtc
     5641 atgcggcaga acatgcgcac gatgaaagcc gcgtggcgtt cggcctcttc gttggacatc
     5701 ttgcgaatgg tggcgcgcac cgcgtcctca ccgcgcaatg gtcgttcgcg ctgcatctcc
     5761 gaagccagtt cgatgaactg aagtcgcaat tcgcgcggta cgtcgtcggg ctcgatgttg
     5821 aacagacatt gacgccaggc accgttcagc cggtccttga tggcaccagc acgggcgagc
     5881 agcagcgtgg ctgattcgag ccgatcgtaa acacgcgaca tgagctgttt cgttatcgct
     5941 cggtgagcgg cggagtccct atctgcgacg caaacctgac gtaacgcctt tagttgttgt
     6001 tgtaacggag accctgatcc gtcaatggcg tacgacgtga atatagcgcg acacctagca
     6061 attgcaacgg gaaatacgca ctgcccgccg gcgacgggcg acaccgaagc acaacgcgcc
     6121 tagcaaaagg acggtcgaga ggtcgagaga acctcccccg ccacctctgc tgccactgcc
     6181 gcgaggagga gtcggggtgg gtgagggcgc tggcgtgacg acaggagtcg ggcccgaagt
     6241 caccggaacc gcgcaggcga tgacacccgc gctcgcggga gcgtcggtgg tcacattgtc
     6301 cgggccgacg accacatccg cggcatcgat ccggcccaga ttgtcggtca ccgtcagtcg
     6361 aacggtgacc gttcccgcac tcggggcaat gacggtggca acggcagttc cgtcgccgcc
     6421 gatcgcgggc gggtcgatcg tgggatcgat caccgaccag gcgtagctgg ccacggtcct
     6481 gccacaggcg gcggcgctgg cggatgcatt cagggtcacg ttctggccgg ccgatacctg
     6541 cacgggaaga gtcacggcgg caatcggcct ctcggcggca gcgacggagc cggccgcgtc
     6601 agccatgccg gcgccgcagg tgctcgtcgt gcaaaggcat tgcgcgagct ggaaatcatc
     6661 atcgtcgacc ggcacgtggc aggtcggaat atccggctcg cctgtcacgg tcgtggggaa
     6721 gggccgtgcg ccctcgcgaa gtcgcgcgag catctgagtg gttgagagat tcgcattgcg
     6781 cgacaacatc aacgccgcaa tgccggagac gatgggcgcc gagaaactcg tgccgagatt
     6841 ggtgttcgtc tggtccgtga agatgtggct cgccgggacg gtcgtaccgc tgttagacgt
     6901 cgtatcgagc gaaaacaggc aaggtccgcc attgatgttg acgcagtttc cgcccggcgc
     6961 ggcgatcgtc acttcgggtc ccaggctgct gaagcccacc ttgctgcccg catgacggat
     7021 cgctgccaca ccggcaacgc cgggacagtt tccgggcgaa gaaaccatcg tcccttcgtt
     7081 gcccgcggaa acgacgagca acacctttcg tgccgtgagt tcctcgatga cttcccggta
     7141 cgaatcctcg caggcacctc cgctgcccag gctggcgttc aggatgcgcg cgggcgtggg
     7201 gttatcgggt acgccgggta catgaaggcc cgccgcccag cgcatgcccg cgaggacgtc
     7261 gctgtcgatc ccgccgcatt tgccgagcac ccgcaccggc aggatgaatg agttccagcc
     7321 gaggccggcg acgcccgacg aattgttagt cagcgctccg atcattccgg cgacgcgcgt
     7381 gccatgccac gaactcccgg tggtgtcgca attggtgaag gccgcatcgg tcttgtcgct
     7441 cgagttgacc cagtcaccgg ggtcggatgg gttcgcgtcg cggccgtcag cgtcattcga
     7501 catgtgctgc aggctcacga agtcgaagcc cgggagcagc tttccaccgc gatcgccacg
     7561 gccgagatcc gggtggtcgt acaaaacgcc cgtgtcgagc actgcaatga ccacgcccga
     7621 tgagccgact tctcggtccc aggcgccgat tgcgttgacc gccgcgggct cggcgtcttt
     7681 caggtaccac tggtttacga acagcgtgtc gttggggttc gtcgcgtgcg cgaaccggcg
     7741 actgtccagc gtcacgtagg ccacggcggg ctcggcacg
//
LOCUS       DATDEU010000399         3083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_658139, whole genome shotgun sequence.
ACCESSION   DATDEU010000399 DATDEU010000000
VERSION     DATDEU010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658139"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VIV63_09505"
     CDS             <1..657
                     /locus_tag="VIV63_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124877.1"
                     /translation="PVVKLKNFRDRWVGSSEGNLEKIFRLIRALGRCMVFIDEADQTL
                     GKRDSGANDSGLSGRIYSMIAQEMSDSGNRGRVLWLLASSRPDLIEVDLKRPGRIDVK
                     VPLLPTSTPAESAQLVGMLARRYGLEIGADDLKRLEPRMPTLLTPGAAEALVVKAYRH
                     ARTQNVPGGAALEASLVGYQNPVPADVLNFQMRIAIREATDLGFVPPAFRAMADAVPE
                     "
     gene            654..1505
                     /locus_tag="VIV63_09510"
     CDS             654..1505
                     /locus_tag="VIV63_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124878.1"
                     /translation="MKKKPSYLSAAFSARPLGMPIPPNWFGLAAFGLLGAIINPGLWL
                     IGAGLEGFYLWWLSKNDRFRATVDAEHGFTDSNTRYEQMLAHLDSSAQSHQYEIEREA
                     TEIVQLLQRSEAHGSQIGDVRQMAWLHLKLLTARATFAEVVEVADRERKKLDEQERRC
                     RQRLEAPDTDEELRRSLEQQIEVITSRRAAHADALRRMELVDAELGRLRQQVSLVREQ
                     ALLVTEEGNIGQSLDSLSASLNEANRWLKDQHELFAGLENMTDEPPPADLLASRRGTK
                     RTTKISE"
     gene            1537..>3083
                     /locus_tag="VIV63_09515"
     CDS             1537..>3083
                     /locus_tag="VIV63_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124879.1"
                     /translation="MSSRHRNARGSAVGNLITLLLLVGIVGLGVWLWLGKKDEPKAAG
                     ESQKSSAAQPAKNDAVATPEGDAPSPIEPVIGTPTLEAANTFVPKDNILRIDISEYAG
                     YGGLIVANGGLEPNADSFFFKEYGFKVAISMSESETWSPLNNGQLAATATTADALAVL
                     GRQFEAVIPIQLGYSRGADMVVVDRGITSVNALGGKILAASQFNESEFFIRYLAQEAG
                     LPVVVLRDLDAKPKAGSLGLVFYEDAFVACDAYQHELAGGGRLNGCVGWTPKTDEVIE
                     ASKGAAKALVSNRNLLVIADVLAVNKGFAKAHPEMVKGLVHGILEGNRRLRDNQAENI
                     AVVAKAFKWSEADARDELAHVHLSNAPENRAFFAGTIDSAGSFGGIFQSSVLAYGSLL
                     RNPADPSRFSDTSYLDAIAKRTPFSEQKIAIAPIRTSAQAALEGDPLLSKDIRFFFEP
                     NSSNLDRSAAQNQEYLQTIKRFLQVSPGSTVLLRGHVDNARVNEFRQSGGDQLVQSMA
                     LKAMELSR"
BASE COUNT          590 a         1040 c          945 g          508 t
ORIGIN      
        1 ccggtggtca agctcaagaa tttccgcgac cgctgggtcg gctcatccga gggcaatctc
       61 gagaagatct tccggctcat ccgcgcgctc ggtcgttgca tggtgttcat cgacgaggcc
      121 gaccagacgc tcggcaaacg cgactcgggc gccaacgatt ccggcctctc cggccgcatc
      181 tactccatga tcgcgcagga gatgtcggac agcggcaatc gcggacgcgt gttgtggttg
      241 ttagcctcgt cgcggccgga cctcatcgaa gtcgacctca agcgacccgg ccgcatcgac
      301 gtcaaggtgc cgctgctgcc gaccagcacc cctgctgaaa gcgcgcagct cgtcggcatg
      361 ctcgccaggc gttacggcct ggagatcggc gcagacgatc tcaagcgcct cgagccccgc
      421 atgcccacgt tgctcacacc cggcgcggcc gaggcgctgg tcgtcaaggc ctaccgtcac
      481 gcgcgcacgc agaacgtgcc cggcggcgcc gcgctcgaag ccagcctcgt gggttatcaa
      541 aaccccgtcc ccgccgacgt gctcaatttc cagatgcgca tcgccatccg cgaagcgacg
      601 gatctcggct tcgtgccgcc ggcgttccgc gccatggccg acgccgtgcc ggaatgaaga
      661 agaagccctc ctatctttcc gccgcgttca gcgcgcggcc gctcggcatg ccgataccgc
      721 ccaactggtt cggtctggcc gcgttcgggc tgctgggcgc aatcatcaac cccgggctgt
      781 ggctcatcgg cgccgggctc gaaggttttt acctgtggtg gctgtcgaag aacgatcgct
      841 tccgcgccac ggtcgacgcg gaacacggct tcaccgacag caacacgcgg tacgagcaga
      901 tgctcgcgca cctcgactcc tccgcgcagt cgcaccagta cgaaatcgag cgtgaagcca
      961 ccgagatcgt ccagctcctg cagcgaagcg aggcgcatgg ctcgcagatc ggcgatgtgc
     1021 gccagatggc gtggctccac ctcaagctgc tgaccgcgcg cgccacgttt gccgaagtcg
     1081 tcgaagtcgc cgatcgcgaa cgcaagaagc tcgacgaaca ggaacggcgt tgccgccagc
     1141 ggctcgaagc gccggacacc gacgaagaac tgcgccgcag cctcgaacag cagatcgaag
     1201 tcatcacgtc gcgccgcgcc gcgcacgccg atgccctgcg ccgcatggaa ctcgtcgacg
     1261 cggaactcgg ccggctgcgc cagcaggtat cgctggtgcg cgaacaggcg ttgctggtga
     1321 ccgaagaagg caacatcggg caatcgctcg actcgttgtc cgcctcgctc aacgaagcga
     1381 atcgctggct caaggatcag cacgaactgt tcgcgggcct cgagaacatg accgacgaac
     1441 caccgccggc ggatctgctg gcttccaggc gcggcacgaa gcgcacgacc aaaatttccg
     1501 agtaagccaa gtttccgagt ccaaggagta gtgaccatgt cttcacgtca tcgcaatgca
     1561 cgcggttcgg ccgtcggcaa cctcatcacg ttgctgttgc tggtcggcat cgtcggtctc
     1621 ggcgtatggc tgtggctcgg caagaaagac gagcccaagg cggcaggtga atcgcagaag
     1681 agcagcgctg cccagccggc gaagaacgat gcggtcgcga ctcccgaagg tgacgcgccc
     1741 tcgcccatcg aaccggtgat cggcacaccg acgctggaag cggcgaatac gttcgtcccg
     1801 aaagacaaca tcctgcgcat cgacatcagc gaatacgccg ggtacggcgg actcatcgtg
     1861 gccaacggcg gcctcgaacc gaatgccgac tcctttttct tcaaggaata cggcttcaag
     1921 gtggcgatct cgatgagcga gagcgagacc tggagcccgc tcaacaacgg ccagctcgcc
     1981 gccacggcga cgacggccga tgcgctcgcg gtgctcggcc gtcagttcga agccgtgatc
     2041 cccatccagc tcggctactc gcgtggcgcc gacatggtgg tcgtggatcg cggcatcacc
     2101 tcggtgaacg cgctgggcgg caagatcctc gccgcttcgc aattcaacga gagcgaattc
     2161 ttcatccgtt accttgcgca ggaagctggc ctgcccgtgg tggtgctgcg tgacctcgac
     2221 gccaaaccga aggccggctc gctgggcctc gttttctacg aagatgcgtt cgtggcctgc
     2281 gacgcctatc aacatgaact cgccggtggc ggacgtctga atggctgcgt cggctggaca
     2341 ccgaaaaccg acgaagtcat cgaggccagc aaaggcgccg ccaaggcgct ggtctcgaac
     2401 cgcaacctgc tggtcatcgc cgacgtgctg gcggtgaaca aaggcttcgc caaggcgcac
     2461 ccggaaatgg tgaagggcct cgtgcacggc attctcgaag gcaatcgccg cctgcgcgac
     2521 aaccaggcgg agaacatcgc cgtcgtcgcc aaggcattca aatggagtga agcggatgcg
     2581 cgcgacgaac ttgcgcacgt gcatctgtcg aacgcgccgg agaatcgcgc gttcttcgcc
     2641 ggcaccatcg actccgccgg ttcgttcggc ggcatcttcc agtcctcggt gctcgcctat
     2701 ggcagcctgc ttcgcaatcc ggccgaccct tcgcgtttct cggatacgag ctacctcgac
     2761 gcaatcgcca agcgaacgcc gttctccgaa cagaagatcg ccatcgcccc gatccgcacg
     2821 tccgcgcagg cggcgctcga aggcgatccg ctgctcagca aggacatccg tttcttcttc
     2881 gagcccaact cctcgaacct ggatcgctcc gcggcccaga atcaggagta cctgcagacc
     2941 atcaaacgat tcctgcaggt cagccccggc tcgacggtgc tgctgcgcgg tcacgtggac
     3001 aacgcccgcg tcaacgaatt ccgtcagtca ggtggtgatc aactggtgca gagcatggcg
     3061 ctgaaggcca tggagctttc acg
//
LOCUS       DATDEU010000400         7914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_364613, whole genome shotgun sequence.
ACCESSION   DATDEU010000400 DATDEU010000000
VERSION     DATDEU010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364613"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1220
                     /gene="gcvT"
                     /locus_tag="VIV63_09520"
     CDS             132..1220
                     /gene="gcvT"
                     /locus_tag="VIV63_09520"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY6124880.1"
                     /translation="MGRRTPLYDLHVEQGARMVDFGGWDMPVNYGSQIEEHHAVRRDA
                     GIFDVSHMCVIDVRGPRVREFFSRLIANDVAKLTQPGKALYSCMLNEAGGVIDDLIVY
                     FMNESWFRTVVNAGTRDKDLAWMRGIAPQFGVELTERVDLAMLAVQGPNARAKVAALL
                     PAEHRDAALALEVFFGRELGPWFVARTGYTGEDGFEIMLPAGEAARVWRTLNEHGVKS
                     AGLGARDTLRLEAAMNLYGNDMDESQNPLESGLSWTVSFENPSRDFIGRKALEALKAA
                     GLAKKFVALLLEDRGVLRGHQKVIVAGAGEGEITSGTFSPTMEKSIALARVPRATGAT
                     VQVDVRGKLLNARVVKAPFVRHGKILVS"
     gene            1244..1630
                     /gene="gcvH"
                     /locus_tag="VIV63_09525"
     CDS             1244..1630
                     /gene="gcvH"
                     /locus_tag="VIV63_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY6124881.1"
                     /translation="MSNVPADLKYTKSHEWVRTLADGNVEVGITDHAQGALGDLVFVE
                     VPEAGKALKAGDSFAVVESVKAASDVYAPVAGAVVEGNAALGSQPELINSEPYGAGWI
                     MRLKPGAAPSGLLSAAEYQAHLAAEG"
     gene            1633..3003
                     /gene="gcvPA"
                     /locus_tag="VIV63_09530"
     CDS             1633..3003
                     /gene="gcvPA"
                     /locus_tag="VIV63_09530"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY6124882.1"
                     /translation="MPFNPHTPRDVSDMLAAIGAKKIEDLFDEIPKELRIESLAGIPD
                     ALNEMMTGKLMMARAAQDGTPLNFIGAGAYEHHIPAAVWAVTTRGEFYSAYTPYQAEA
                     SQGTLQLIYEYQTMISSLTGMEVSNASLYDGGSAVAESSLMAIRAHRKSKSQRILVPT
                     TVHPNYRKVAVATATGQGVRFEEVPCPKGFLSVADLAKYQGEDITAIVVQQPNFFGVL
                     EDVDAITDWAHAHGIMVIGSVNPTSLAILKPPGEWGAKGADIACGDCQPLGVPLSSGG
                     PYAGFLTTRMEYVRQMPGRIVGRTVDANGQQGFALTLQAREQHIRRGKATSNICTNQG
                     LLVTAATIYLSLLGPEGLARVANESHARTGELVAALTRIKGVKLAFDRPRFHEAVLLL
                     DRPVAPVIAELARRGIVGGFDLSVDYPELGHALLVCATETRTHTDIQLYADALNETLK
                     AAVRAA"
     gene            3015..4502
                     /gene="gcvPB"
                     /locus_tag="VIV63_09535"
     CDS             3015..4502
                     /gene="gcvPB"
                     /locus_tag="VIV63_09535"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEY6124883.1"
                     /translation="MAMQKHPEKIIFEYSRPGRGAFGQWPTEPGTDNEVAADIPENLR
                     RKKPAMLPEVSELEVVRHFTKLSQLNFSIDTHFYPLGSCTMKYNPKACNSFAMLPGFL
                     NRHPLAPESLGQGFLACMYELQEMLKEVTGMQGVALSPMAGAHGEFAGVAMIRAYHRA
                     RGDLARNEIIVPIAAHGTNPATATMCGCVVKEIPVNDEGDVDVEALRAAVGPNTAGIM
                     LTNPSTCGVFERRIKEVAKIVHDAGGLLYYDGANLNAILGKVRPGDMGFDVIHMNLHK
                     TFSTPHGGGGPGAGAVGVSSRLLPFMPIPVVGKEGDKYRWLTEKDLPQSIGRLSAFMG
                     NAGVLLRAYIYMRMLGREGMHRVGEFATLNANYLMARLAKAGFDPAYPTRRASHEFII
                     TAKKQKNELGVSAMDIAKRLLDYGFHAPTTYFPLLVPECLLIEPTETESKEALDGFID
                     AMVSILKEAETQADLVKSAPHTMTVRRLDDVRAARQLDLTWKPAA"
     gene            4594..4968
                     /locus_tag="VIV63_09540"
     CDS             4594..4968
                     /locus_tag="VIV63_09540"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY6124884.1"
                     /translation="MNDTGDRQKPTGFTRMFRAFGASMMGFAGAFREEAAFRQELALA
                     VVVIPLGMWLGRSGIERALLIAPMFLVLIVELINSAIEATVDRIGLERHKLSGLAKDI
                     GSAAVLTSLLLLAVIWLLILWR"
     gene            4995..7865
                     /locus_tag="VIV63_09545"
     CDS             4995..7865
                     /locus_tag="VIV63_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY6124885.1"
                     /translation="MQPRLLLIGLILFTSALSQQAGAQNAPPQVVSQSQATQAALPQT
                     EGESAAWRGTLARVAESVVAIEIDQTRAFDTEWNASAQATGFVVDAERGLILTNRHVV
                     TPGPVTSEATFLNREEVQLYPVYRDPIHDFGIYRYDPKKLRFIKPRSLPLYPDGAQIG
                     REIRVIGNNAGEQLSILAGTIAKLDRDAPEYGIGKYNDFNTFYLQAASGTSGGSSGSP
                     VIDIQGRVVALNAGGATGAASSFYLPLGRVKRALDLIQAGKSVSRGALQVVFHYTPYD
                     ELRRLGLTEKTEESARKAFPALTGMLVVNEVLPGGPADGTLQPGDVLVRVNGKLVSEF
                     EPLEQVTDESVGGTVDLDLERGGKPIAAKLNVGDLHEITPSAYLEFGDSVVHTVSYQQ
                     ARHFNVAVSGVYVANPGYIFGAAGVPRGAVISSANGKPTPNLAAFEAVVAGLADGERF
                     TVRYSTVDDPNGSSVRSARMDRRWFPANHCLRDDAAGVWQCAALPALGSAKPEVGGST
                     RFSKPDDPRAASLAASLVGVGFDMPYSVSGITERNYRGTGLVVDAARGLVVVDRNTVP
                     VSMGDVRVVFAGTLEIPGKVEFVHPLHNLAIVSYDPKLVGTTPVRSAKLLDRKITLGE
                     SLWVVGMGPDSELNTRATQVASLSPIQLPLSRTMQFRESNLETIQLVNGPADFDGVLT
                     DADGAIVGLWSSFAWEAGRELQQENRGVAIGVVADMLRRVQAKQPLSSLEAEFVPQPL
                     ASARRLGLPDTWLRKFEAASPNQRQVLSIVRLVGGSPAVGKLKQGDILLSIDDKVVTK
                     FREVELAVADKPEVNVMVWRDGEEQLVTLATAALTGSDIDRIVMWAGATLQAPHRAMI
                     AQRGIPPEGVYVGYFAYGSPATRYGLFPGRRITEVDGIPTPNLDAFLKVVSGRPDRSA
                     IRLRTITWNNAPEVITLKLDRHYWPTYELQRTDSGWQRRDLE"
BASE COUNT         1413 a         2628 c         2524 g         1349 t
ORIGIN      
        1 catttacttg taacaatcgt ctgagtgatt gccacgtcga gttcgtggcc gattgttaca
       61 agtaaatgag gaatgtcccc atttcccgcc ttttttattg gcacgcaccg cgggcggcca
      121 gggagaggtt cttgggcaga cgcacacctt tatatgacct gcacgtcgag cagggcgctc
      181 gcatggtcga cttcggcgga tgggacatgc ccgtcaatta cggctcgcag atagaagagc
      241 accacgcggt gcgtcgcgat gctggcatct tcgacgtgtc acacatgtgt gtcatcgatg
      301 tccgcggccc acgggtccgc gaattcttct cccggctcat cgccaacgac gtcgccaagc
      361 tcacgcagcc gggcaaggcg ctctatagct gcatgctcaa cgaggcgggc ggcgtcatcg
      421 acgacctcat cgtctacttc atgaacgagt cgtggttccg cacggtggtc aacgccggca
      481 cgcgcgacaa ggacctggcc tggatgcgcg gcatcgcgcc gcaattcggc gtggagctca
      541 ccgagcgcgt cgatctcgcc atgctcgccg tgcagggtcc gaacgcgcgc gcgaaagtgg
      601 ccgcgctgct gcccgccgaa catcgcgacg cggcgctggc gctcgaagtg ttcttcggcc
      661 gcgaactcgg gccctggttc gtcgcgcgca ccggctacac cggcgaagac ggtttcgaaa
      721 tcatgctgcc ggccggtgaa gccgctcgcg tgtggcgcac gctcaacgag cacggcgtga
      781 aatcggcggg cctgggcgcg cgcgacacgt tgcgactcga agcggcgatg aacctctacg
      841 gcaacgacat ggacgagagc cagaatccgc tcgagtcggg gctctcctgg accgtgtcgt
      901 tcgagaaccc ctcgcgtgac ttcatcggac gcaaggcgct cgaagcactg aaggccgcgg
      961 gtctcgcgaa aaaattcgtg gcgctgctgc tcgaagaccg cggcgtgttg cgcgggcacc
     1021 agaaggtcat cgtcgccggc gcgggtgaag gtgagatcac cagcggcacg ttctcgccca
     1081 cgatggaaaa gtcgatcgcg ctcgcgcgag tgccgcgcgc caccggcgcc accgtgcagg
     1141 tcgatgttcg cggcaagtta ttgaacgctc gcgtggtgaa agccccgttc gtgcgtcacg
     1201 gcaagatact cgttagttag tccactcttt gcggagcaaa ttcatgagca acgtcccggc
     1261 ggatctgaag tacaccaagt cgcacgagtg ggttcgcacg ctcgccgacg gcaacgtcga
     1321 agtgggcatc accgaccatg cgcagggtgc gctcggcgat ctcgtgttcg tcgaagtgcc
     1381 ggaggccggc aaggcgctca aggccggcga ctcgttcgcc gtggtcgaat cggtcaaggc
     1441 cgcctccgac gtgtacgccc cggtcgcggg cgccgtcgtc gaaggcaatg ctgcgctcgg
     1501 cagccagcct gaactgatca actccgagcc ttacggcgcg ggctggatca tgcgtctcaa
     1561 gccgggcgcc gcgccttccg gattgctgag cgccgccgag taccaggcgc atctggccgc
     1621 ggagggttaa cgatgccatt caatccgcac acgcccaggg atgtgagcga catgctcgcc
     1681 gccatcggcg cgaagaaaat cgaagacctg ttcgacgaga tcccgaaaga gctgcgcatc
     1741 gagtcgctgg ccggcattcc ggacgcgctc aatgaaatga tgacgggcaa gctgatgatg
     1801 gcgcgcgccg cgcaggacgg cacgccgctc aacttcatcg gcgccggcgc ctacgagcac
     1861 cacatcccgg cggcggtgtg ggccgtgacc acgcgcggcg agttctacag cgcctacacg
     1921 ccctaccagg cggaagcgtc gcagggcacg ctgcagctca tctatgaata ccagacgatg
     1981 atttcctcgc tcaccggcat ggaagtctcg aacgcgtcgc tgtacgacgg cggctctgcc
     2041 gttgccgaat cctcgctcat ggcgattcgc gcgcatcgca agagcaagtc gcagcggatc
     2101 ctcgtgccga ccaccgtgca tccgaactac cgcaaggtgg ccgtggcgac ggcgacgggc
     2161 cagggtgtgc gtttcgagga agtgccgtgc ccgaaaggat tcctgtcggt cgccgacctc
     2221 gcgaagtatc agggcgaaga catcaccgcc atcgtcgtcc agcagcccaa cttcttcggc
     2281 gtgctcgaag acgtcgacgc catcaccgat tgggctcatg cccacggcat catggtcatc
     2341 ggctcggtga atcccacctc gctcgccatc ctcaagccgc cgggtgagtg gggcgccaag
     2401 ggcgccgaca tcgcctgcgg cgattgccag ccgctcggcg tgccgctgtc ctcgggtggt
     2461 ccttacgccg gcttcctcac cacgcgcatg gaatacgtgc gccagatgcc gggacgcatc
     2521 gtcggccgca ccgtcgacgc caacgggcag cagggcttcg cgctgacgct gcaggcccgc
     2581 gagcagcaca tccgtcgcgg caaggccacg tcgaatatct gcaccaacca gggcctgctg
     2641 gtcacggccg cgacgatcta cctgagtttg ttaggaccgg aaggtcttgc ccgcgtagcg
     2701 aacgaatcgc atgcgcgcac gggcgaactc gtcgcggcgc tcacgcgcat caaaggagtg
     2761 aagctggcgt tcgatcgtcc gcgcttccac gaagccgtgc tgctgctgga ccgtccggtc
     2821 gcacccgtca tcgcggagct cgcacgacgc ggcatcgtcg gcggcttcga cctcagcgtc
     2881 gattatccgg agctgggtca tgccctgctg gtctgcgcca ccgagacgcg gacccacacg
     2941 gacatccagc tctacgccga cgcgctcaac gaaaccctca aagccgccgt gcgcgccgct
     3001 taagggagac acacatggca atgcagaaac atcccgaaaa gatcatcttc gaatactcgc
     3061 ggcccggccg cggcgcgttt gggcaatggc ccaccgagcc cggcaccgac aacgaagtcg
     3121 ccgccgacat tcccgagaac ctgcgccgca agaagccggc gatgttgccg gaagtgagcg
     3181 agctcgaagt cgtgcgccac ttcaccaagc tctcgcagct caacttctcg atcgacacgc
     3241 acttctatcc gctcggttcg tgcacgatga agtacaaccc gaaggcgtgt aactcctttg
     3301 cgatgctgcc ggggttcctc aatcgtcatc cgctggcgcc ggagtcgctc ggccagggct
     3361 tcctcgcctg catgtacgaa ctccaggaga tgctcaagga agtcaccggc atgcagggcg
     3421 tggcgctctc gccgatggcc ggcgcgcacg gcgagttcgc cggcgtcgcc atgatccgcg
     3481 cctatcaccg cgcgcgcggc gatctcgccc gcaatgaaat catcgtgccg atcgcggccc
     3541 acggcaccaa tcccgccacc gccaccatgt gcggctgcgt ggtcaaggaa attcccgtca
     3601 acgacgaagg cgacgtcgac gtcgaagcgc tgcgtgcggc ggtcggaccc aacaccgcgg
     3661 gcatcatgct cacgaatcct tccacgtgcg gcgtgttcga acggcgcatc aaggaagtcg
     3721 cgaagatcgt ccacgatgcg ggcggcctgc tgtattacga cggcgccaac ctcaacgcca
     3781 ttctcggcaa ggtccggccc ggcgacatgg gtttcgacgt catccacatg aacctgcaca
     3841 agaccttctc cacgccgcac ggcggcggcg ggccaggtgc gggcgcggtg ggtgtgagct
     3901 cacgcctgct gccgttcatg cccatccccg tggtgggcaa ggaaggcgac aagtaccgct
     3961 ggctcaccga aaaggatctg ccgcagtcca tcggccgact ctcggcgttc atgggcaacg
     4021 ccggcgtact gctgcgcgcg tacatctaca tgcgcatgct gggccgcgaa ggcatgcacc
     4081 gcgtcggcga gttcgcgacg ctcaacgcca actacctgat ggcgcggctg gcgaaagcgg
     4141 gtttcgatcc cgcgtatccg acgcgtcgcg cgagccacga gttcatcatc acggcgaaga
     4201 agcagaagaa cgagctgggc gtttcggcca tggacatcgc caagcgattg ctcgactacg
     4261 gcttccacgc gcccactacc tactttccgc tgctggtgcc ggagtgcctg ctcatcgagc
     4321 ccaccgagac cgagagcaag gaagcgctgg acggtttcat cgatgcgatg gtgtcaatcc
     4381 tcaaggaggc ggagacccag gccgatctcg tgaagtccgc gcctcacacg atgaccgtac
     4441 gccggctgga tgacgtccgc gcggcgcgtc agctcgatct cacctggaag ccggcagcgt
     4501 aggaaagggc gaagggcccc tttatcgata cggacgccgg cccttcaggc cggcgtttcc
     4561 gatgtggggc gcagcccctg ataagatcgc aacatgaacg acaccggaga ccgacagaaa
     4621 cccaccggct ttacgcgtat gttccgcgcg ttcggcgcga gcatgatggg gttcgcgggc
     4681 gcgtttcgcg aggaggccgc cttccggcag gaactcgccc tggcggtcgt ggtcattcct
     4741 ctgggaatgt ggttaggtcg cagcggcatc gagcgtgccc tgctgatcgc ccccatgttc
     4801 ctggtgctga tcgtcgagct gatcaacagc gccatcgagg ccacggtcga tcgcatcggc
     4861 ctcgagcgcc acaagttatc gggtctcgcc aaggacatcg ggtccgcggc ggtactcacg
     4921 tcactgctgc tgctggcggt gatctggctc ctgatcctgt ggcgctaagc ttctaacaag
     4981 acgaaggaca ctgcatgcaa ccacgtctgc tgttgatcgg cctcatcctc ttcacatcgg
     5041 ccctctcgca gcaggcgggt gcgcagaacg cgccaccgca ggtcgtgtcg caatcccagg
     5101 ccacgcaggc cgcgctgccc cagaccgagg gtgaaagcgc cgcctggcgc ggtacgctgg
     5161 cgcgcgttgc cgaaagcgtc gtcgccatcg aaatcgacca gacgcgcgcg ttcgacaccg
     5221 aatggaacgc ctcggcgcag gccacgggct tcgtcgtcga tgctgaacgc ggtctcatcc
     5281 tcaccaaccg ccacgtcgtg acacccggcc cggtcacctc ggaggccacg ttcctcaatc
     5341 gcgaggaagt gcagctttac ccggtctatc gcgatccgat ccacgacttc ggcatctacc
     5401 gctatgaccc gaagaagctg cggttcatca agccgcggtc gctgccgctg tatccggacg
     5461 gcgcgcagat cgggcgcgag atccgcgtca tcggcaacaa cgccggagag cagctctcca
     5521 tcctcgcggg aaccatcgcc aagctggatc gcgatgcgcc cgagtacggc atcggcaagt
     5581 acaacgactt caacactttc tacctgcagg cagcgtccgg tacctcgggc ggttcctccg
     5641 gctcgccggt catcgacatc cagggacgcg tggtcgcgct gaatgccggc ggcgccaccg
     5701 gtgcagcctc gagcttctat ctaccgctcg gacgcgtgaa acgcgcgctc gacctcatcc
     5761 aggcgggcaa gtcggtgtcg cgcggcgcgt tgcaggtggt gttccactac acgccgtacg
     5821 atgaattgcg ccgcctcggc ctcaccgaga aaaccgagga atccgcgcgc aaggcattcc
     5881 cggcgctcac cggcatgctg gtcgtcaacg aagtgctgcc gggcggcccg gccgacggaa
     5941 cgctgcagcc tggcgacgtc ctggtgcgcg tgaacggcaa gctggtgtcg gagttcgaac
     6001 cgctggagca ggtgaccgac gaatcggtcg gcggcacggt ggacctcgac ctcgagcgcg
     6061 gcggcaagcc cattgccgcg aaactcaatg tcggcgatct ccacgagatc acgccttcgg
     6121 cctatctcga gttcggtgac tcggtggttc ataccgtgtc ctatcaacag gcgcgccatt
     6181 tcaatgtcgc ggtgagcggc gtctacgtcg cgaacccggg ttacatcttc ggcgcggcgg
     6241 gcgtgccgcg cggcgcggtg atctccagcg ccaatggcaa gcccacgccc aatctcgcgg
     6301 ccttcgaagc ggtggtggcg ggcctcgccg atggcgagcg tttcacggtt cgatattcca
     6361 cggtggacga tccgaacggc tcgagtgtgc gcagcgcgcg catggaccgc cgctggttcc
     6421 ccgcgaacca ttgcctgcga gatgacgccg cgggtgtatg gcagtgcgcg gcgctgccgg
     6481 cgctgggctc ggccaaaccc gaagtgggcg gcagtacacg gttctccaag cccgacgatc
     6541 cgcgcgcggc ctcgctggcc gcatcgctcg tgggcgtagg tttcgacatg ccgtactcgg
     6601 tctcgggcat caccgaacgc aactatcgcg gcacgggcct ggtagtggat gcggcgcgtg
     6661 gtctcgtggt ggtcgaccgc aacacggttc ccgtttccat gggtgacgtg cgcgtggttt
     6721 tcgccggcac gctcgagatt cccggcaagg tcgagttcgt ccatcccctg cacaatctgg
     6781 ccatcgtcag ttacgacccc aagctcgtcg gaaccacgcc ggtgcgcagc gccaagttgt
     6841 tagaccggaa gatcacgctc ggcgaatcgc tgtgggtggt cggcatgggt cccgacagcg
     6901 agctcaatac ccgcgccacg caggtggcgt cgctgtcgcc catccagttg ccgctgtcgc
     6961 gcaccatgca gttccgcgag agcaatctcg agaccatcca gctcgtcaat ggtccggcgg
     7021 atttcgacgg cgtgctcacc gatgccgacg gcgcgatcgt cggcctgtgg tcgagcttcg
     7081 cctgggaagc gggccgcgag cttcagcagg agaatcgtgg cgtggccatc ggcgtggtcg
     7141 cggacatgct tcggcgcgtg caggcgaaac agccgctgtc ttcgctcgaa gcggagttcg
     7201 tgccgcagcc gctggcgtct gcgcggcgtc tcggacttcc cgacacctgg ttgcgcaagt
     7261 tcgaggccgc gagccccaat cagcgccagg tgttgtccat cgtacggctg gtcggcggat
     7321 cgcccgcggt cggaaagctc aagcagggcg acatcctgct gtccatcgac gacaaggtgg
     7381 tcaccaagtt ccgcgaggtg gaactcgccg tggccgacaa gcccgaagtg aatgtcatgg
     7441 tgtggcgcga tggcgaagaa cagctggtca cgctggccac cgcggcgctc accggcagcg
     7501 acatcgaccg catcgtgatg tgggccggcg cgacgctgca ggcgccgcac cgcgcgatga
     7561 tcgcccagcg cggcataccg cccgagggtg tgtacgtggg gtacttcgcg tatggctctc
     7621 cggccacgcg ttacggcctg ttccccggcc ggcgcatcac cgaagtagat ggcatcccca
     7681 cgccgaatct cgacgcattc ctcaaggtgg tttcaggcag gccggaccga tcggccatcc
     7741 gtctgcggac catcacctgg aacaacgccc cggaagtgat cacgttgaaa ctcgatcggc
     7801 attattggcc gacctacgag ctccagcgca ccgacagcgg ctggcagcgt cgtgacctcg
     7861 aatagagtcg gtacaatgcc cgcctatggt ccgagtcgtt cgagcaaccc ttgt
//
LOCUS       DATDEU010000401         4505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_74160, whole genome shotgun sequence.
ACCESSION   DATDEU010000401 DATDEU010000000
VERSION     DATDEU010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74160"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VIV63_09550"
     CDS             complement(<1..717)
                     /locus_tag="VIV63_09550"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY6124886.1"
                     /translation="MRRKIIALLVPLVGLLTVDAALAAKKAANAPLLKLFEASWQEDL
                     ADDPITATALGDHRYDDKLTDMSVEAITKRSQRAYARLAALRKISKEKLEKADQLNYD
                     LFERETRSVLDEGQYKPYLYDIRTIDGPQLLPELAETHPFQTVKDYDNWIARINASGV
                     YFDQWIVLLGQGAAERRTQPRVTIAKVLDQIKPQLVTNPEDSGFYAPFKKMPASFDAA
                     TKDRLTAAAKTAIQAVAVPAF"
     gene            complement(727..1215)
                     /locus_tag="VIV63_09555"
     CDS             complement(727..1215)
                     /locus_tag="VIV63_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124887.1"
                     /translation="MSEAPVTEIIVEPDHFDVVSTDVVLVAQLESTFALCLYDAVLES
                     GALIHLRVAPPGRVQDPNLTDTTLSTDLLLLDRCFVELRKSEPRAQHWQAKVVGQVQD
                     LPGARERFDGIQSFLSAFLADANVKLVSCDTHVDHVQLLKFRPAMGHVRSEPLAAQRL
                     GS"
     gene            1457..1885
                     /locus_tag="VIV63_09560"
     CDS             1457..1885
                     /locus_tag="VIV63_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6124888.1"
                     /translation="MLSVQQLLDHKPRAIFSIGPEEPVLAAIKKMAEHHIGALVVMSG
                     EQLAGIVSERDYARKVVLLGRSSEETLVSAIMTAKVVTVTPKQDAHDCMRLMTDMRIR
                     HLPVVSGDRVVGVLSIGDLVRAVIEEQERTIADLESYIRS"
     gene            complement(1943..3391)
                     /locus_tag="VIV63_09565"
     CDS             complement(1943..3391)
                     /locus_tag="VIV63_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEY6124889.1"
                     /translation="MLAVAHLLGLMMAFFGLLYLLPIAWSLGVRDGAVMDFVIAAAIN
                     AGAGLAVALATRRFRRELKPRDGFLLVTLSWVLMSASAAIPLMIALPQLSFTDAYFEA
                     MSGLTTTGSTVLNHLDDLPQSINLWRHTLHWIGGIGIIVLVVAVLPLLGVGGMQLYKA
                     ETPGPVKDEKLTPRITETAKALWFTYLAITATGVIGLRIAGMSWFDAICHGFSAIALG
                     GFSTHDVSVGYFDSFAVEVVLIVIMVVAALNFSRHFLAFRSLSFKPYRSDSEGKAVLI
                     VLGSSVVLVAALLAIDGTYPTTMEGFRHSVFNVVSIATTTGFVTEDYEKWPAFLPVWL
                     LFLSCITCSTGSTGGGIKMFRTLLLVRQARRELKLMVHPSAVIPIRIGGNAIPERVVH
                     SVLAFIFLYFGTILVLTFAQLATGLDLVSSFSAVVGSVNNVGPGLGAVGPSTNFSALT
                     DAQTWICTMAMLIGRLEIFSVLVLFTAHFWRK"
     gene            complement(3413..>4505)
                     /gene="trkA"
                     /locus_tag="VIV63_09570"
     CDS             complement(3413..>4505)
                     /gene="trkA"
                     /locus_tag="VIV63_09570"
                     /inference="COORDINATES: protein motif:HMM:NF007030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEY6124890.1"
                     /translation="TKIARIRSSEYTKHPKLFADEALSVDVWISPEQLVTEYIGELIK
                     YPGALQVLDFAEGRVRLVGIRARKGGLLVSQKLRALKEHHPNTEARVVAVYRAGRSII
                     PDGDTVMEEGDEVFFIAARDDIRKFMSEIRKEEAPAKRVVIAGGGNIGLSLARKLEEQ
                     HHVKLIERDPKRARRVSELLLNTIVLNGDAADEELLIEENIDSCDVFAALTNSEEANI
                     LSAMLAKRLGAKKVMALINKPSYADLMESGSIDIAISPQTITIGSLLAHVRRGDVVRV
                     HSLRRGAAEALEAVVHGTDKTSRVVGRKVADIPLPEGAAIGAVVRGDAVMIAHHDTMI
                     HNDDHVIVFLSDRRHVEQVERLFSGPGGR"
BASE COUNT          870 a         1385 c         1460 g          790 t
ORIGIN      
        1 gaaggccggc acggccaccg cctggatcgc ggtcttggca gcggcggtca ggcggtcctt
       61 cgtggcggcg tcgaagctcg cgggcatctt cttgaacggc gcgtagaagc cgctgtcttc
      121 cgggttcgta acgagctgcg gcttgatctg gtcgagcacc ttcgcgatgg tgacgcgcgg
      181 ctgggtgcgg cgttcggcgg cgccctggcc taacaacacg atccactggt cgaagtacac
      241 gccgctggcg ttgatgcgcg cgatccagtt gtcgtagtcc ttgaccgtct ggaacgggtg
      301 agtctcggcg agctcgggca ggagctgcgg gccgtcgatg gtgcgaatgt cgtatagata
      361 gggcttgtac tgcccttcgt cgagaacgct tcgggtctcg cgctcgaaca ggtcgtagtt
      421 gagctggtcg gctttctcga gtttctcttt ggaaatcttg cgcagcgccg ccaggcgggc
      481 gtacgcgcgc tggctgcgct tggtgatcgc ttcgaccgac atgtcggtga gcttgtcgtc
      541 gtatcgatgg tcgccgagcg cggtcgcggt gatggggtcg tcggccaggt cctcctgcca
      601 gctggcttca aacagcttca gcagcggcgc gttggcagcc ttcttggcgg cgagggcggc
      661 atcgacggtg agcaacccca cgagggggac cagcaaggca atgatttttc ggcgcatggc
      721 cgattttcag ctacccaggc gctgagcggc aagaggttcg ctgcgaacat gtcccatggc
      781 gggacgaaac ttcagcaatt gcacgtggtc cacgtgggta tcgcaggaca ccagcttcac
      841 attcgcgtcc gcaaggaagg ccgagaggaa gctctggatg ccgtcgaatc gctcgcgcgc
      901 gccgggcagg tcctggacct ggcctacgac tttcgcctgc caatgctgcg cccgcggctc
      961 gctcttgcgc agctcgacga agcagcgatc cagcagaagc aggtctgtcg acagcgtcgt
     1021 atcggtgaga ttggggtctt gcacgcggcc cgggggcgcc acacgcaggt gaatcagtgc
     1081 cccggattcg agcaccgcgt catacagaca taacgcgaac gtggattcga gctgggcgac
     1141 cagtacgaca tcggtggata cgacatcgaa gtgatcgggc tcgacgatga tttccgtaac
     1201 gggggcttct gacacggcga cctgatacta agacgttatc cccgggctgg cgcgtcagct
     1261 tctgtcggct ggcgtttcgc tggcatccct agagtcgagg atcatcgcag actcggcgca
     1321 gcattcccat gtcgcagggc agctacgtgc ggaaaaggcg acccggagcg tatgagtccc
     1381 gcgattcgag cgcgggactc ggggaacacc cgggtcgggc cgtctacgcc acgattcacg
     1441 agatacggag gtcgcgatgc tttccgtgca gcaactgctg gaccacaaac ccagggccat
     1501 cttttcgata gggcccgaag aacccgtgct cgccgccatc aagaagatgg ccgagcatca
     1561 cattggcgca ctggtggtga tgtccggcga acagctggcc ggcatcgtct ccgagcggga
     1621 ctatgcgcgc aaagtcgtgt tgttaggccg gtcctccgaa gagacgctgg tctcggccat
     1681 catgaccgcg aaggtggtca ccgtgacgcc gaaacaggat gcgcacgact gcatgcggct
     1741 catgaccgac atgcgcatcc gccatctgcc ggtggtgtcc ggcgatcgcg tggtgggtgt
     1801 gttgtccatc ggtgacctgg tgcgcgccgt catcgaagag caggaacgca cgattgcgga
     1861 cctcgagagc tacatccgta gctagagcaa aaaggggaca gatctatttt ccgctgcgga
     1921 aaatagatct gtcccctttt tgctacttcc gccagaagtg cgcggtgaac agcaccagca
     1981 cgctgaagat ttccaggcgg ccgatgagca tggccatcgt acagatccag gtctgcgcat
     2041 cggtcagtgc cgagaagttg gtcgaaggac ctaccgcgcc aaggccaggg cccacgttgt
     2101 tcaccgaacc cacgaccgcc gagaacgacg acaccaggtc gagccccgtc gcaagttggg
     2161 cgaacgtcag cacgaggatc gttccgaaat aaaggaagat gaatgccagc accgaatgca
     2221 ccacgcgctc gggaatggcg ttgccgccga tgcggatggg aatcacggcg ctgggatgca
     2281 ccatcagctt tagctcgcgg cgcgcctggc ggaccagcag cagcgtgcgg aacatcttga
     2341 tgcctccacc cgtagagccg gtgctgcagg tgatgcagct caggaacagc agccagaccg
     2401 gcaggaatgc cggccacttc tcatagtcct ccgtgacgaa gccggtagtg gtggcgatgg
     2461 acacgacgtt gaacaccgaa tggcgaaaac cttccatggt cgtcggatag gttccgtcga
     2521 tcgcgagcag cgccgccacc agcaccaccg acgagccgag cacgatgagc acggccttgc
     2581 cctcggaatc tgagcgataa ggcttgaatg acagactgcg gaacgcgaga aagtgacgcg
     2641 agaaattcag cgccgccacg accatgatga cgatcaacac cacttccaca gcgaaggaat
     2701 cgaagtaacc cacgctcacg tcatgtgtcg agaacccacc gagggcgatg gctgagaaac
     2761 cgtggcatat cgcgtcgaac cagctcatgc cggcaatgcg caggccgatt acgccggtcg
     2821 ccgtgattgc gaggtacgtg aaccagagag ccttcgccgt ttccgtgatg cgcggtgtga
     2881 gcttctcgtc cttgaccgga ccgggcgtct ccgccttgta gagctgcatg ccgcccacgc
     2941 ctaacaaggg caggacggcg acgaccagca cgatgatgcc gatgccgccg atccagtgca
     3001 gcgtgtggcg ccagagattg atggactgcg gcagatcgtc gagatgattc agcacggtgg
     3061 aacccgtggt ggtcaggccc gacatcgcct cgaaatacgc atcggtgaac gagagctgcg
     3121 gcagcgcgat catcagcggg atcgcggccg acgcggacat cagcacccac gacagcgtga
     3181 cgagcaggaa accatcgcgc ggcttgagct cgcggcggaa gcgccgcgtg gccaacgcca
     3241 ccgcgagacc ggcgccggcg ttgatggcgg cggcaatcac gaagtccatg accgcgccat
     3301 cgcgcacgcc cagcgaccag gcaatgggca gcaggtagag aaggccgaag aaggccatca
     3361 tgagcccgag cagatgcgcg acggctaaca agggctcaaa gctccggagc gctcaacggc
     3421 cgccaggccc ggagaacagg cgctcgacct gctccacgtg gcgccggtcg gacaggaaaa
     3481 cgatgacgtg atcgtcgttg tgaatcatgg tgtcgtgatg cgcgatcatc accgcatcgc
     3541 cccgcaccac cgcgccgatg gccgcgccct cgggcagcgg aatatccgcc accttccgac
     3601 cgaccacgcg cgaggtcttg tcggtgccgt gcaccacggc ctcgagcgct tcggcagcgc
     3661 cgcgacgcag gctgtgcacg cgcaccacgt caccgcgacg cacgtgcgcg agcagcgaac
     3721 cgatggtgat ggtctgcggg gaaatggcga tgtcgatact gccgctctcc atgagatcgg
     3781 cataagaagg cttgttgatg agcgccatca ccttcttcgc acccagccgc ttcgcgagca
     3841 tggccgagag gatgttggcc tcttcggagt tcgtcagcgc cgcgaacacg tcgcagctgt
     3901 cgatgttctc ctcgatcagc agctcttcgt cggcggcgtc gccattgagc acgatggtgt
     3961 tgagcagaag ctccgacacg cgccgcgcac gtttcggatc acgctcgatg agcttcacgt
     4021 ggtgctgctc ttcgagcttg cgcgccagcg acagaccgat gttgccgccg cccgcgatca
     4081 cgacgcgctt cgccggcgct tcttccttgc gaatctcgct catgaacttg cggatgtcgt
     4141 cgcgcgcggc aatgaagaag acctcgtcgc cttcctccat gacggtgtcg ccatcgggga
     4201 tgatgctgcg cccggcgcga tagaccgcca cgacgcgcgc ttcggtgttc gggtgatgtt
     4261 ccttgagcgc gcgcagcttc tgcgagacca gcaggccacc cttgcgtgcg cggataccca
     4321 ccagccgcac gcggccttcg gcgaaatcga gtacctgcag cgcacccgga tacttgatga
     4381 gctcgccgat gtattcggtg acgagctgct cggggctgat ccacacgtcc accgacaacg
     4441 cttcgtcggc gaacaacttc ggatgcttgg tgtactcgga cgaccggatg cgcgcgatct
     4501 tcgtg
//
LOCUS       DATDEU010000402         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1097033, whole genome shotgun sequence.
ACCESSION   DATDEU010000402 DATDEU010000000
VERSION     DATDEU010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097033"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="rpsB"
                     /locus_tag="VIV63_09575"
     CDS             complement(<1..305)
                     /gene="rpsB"
                     /locus_tag="VIV63_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY6124891.1"
                     /translation="MSGVSMRQMLEAGVHFGHQTRFWNPKMAPFIFGERNKIHIINLE
                     KTQPMYSEAATFVKGVVADGGKVLFVGTKRSAREAVEKEARRCEMPFVNQRWLGGMLT
                     "
     gene            550..1350
                     /gene="map"
                     /locus_tag="VIV63_09580"
     CDS             550..1350
                     /gene="map"
                     /locus_tag="VIV63_09580"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY6124892.1"
                     /translation="MDSLVSRHITIKSPAEQEKMRVAGRLAAEVLDMIGEHVKPGVTT
                     EELDRLCFEHITQVQKSIPANVNYRGFPKTICSSVNHVVCHGIPNDKALKSGDILNID
                     VTVIRDGFHGDTSRMYYVGKPALPAQRLAESCWEAMWRGIEKVKPGARLGDIGHAIQT
                     FVEDRNFSVVREYCGHGIGRVYHEDPQVLHYGEPGTGDELKAGMTFTIEPMVNAGKRH
                     VRLLGDGWTVVTKDHSLSAQWEHTCLVTENGVEVLTLGEGSMRPAASN"
     gene            1351..>2305
                     /locus_tag="VIV63_09585"
     CDS             1351..>2305
                     /locus_tag="VIV63_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY6124893.1"
                     /translation="MASLEEGIEETETTVLRSTPWMRLANLPERLAEAKFAPDAFRLE
                     LKEANEELAERFRADESVEVLVHARAELIDVILREVWRSQLPAGYESWTLAAVGGYGR
                     GELHPHSDVDILILVPATPDDAGRGVVERVVTFLWDINLEVGHSVRTVAQCAEESVAD
                     VGVMTTLVEARLLAGNAELLAEMREAVSADRVWSIKQYFEAKVAEQQDRHLKANDTAY
                     NLEPNVKTGPGGLRDIQTIAWVAKRHFGASSLDELLTHGFLSFTELRKLKQAQSFLWK
                     VRFGLHIVTGRREDRLLFDHQIKLAQMFGYEDASYTLAVEQF"
BASE COUNT          456 a          732 c          708 g          409 t
ORIGIN      
        1 gtgagcatgc cgccaagcca acgctggttg acgaagggca tctcgcaacg tcgagcttct
       61 ttctcgaccg cttcacgcgc cgagcgcttg gtaccgacga acagcacttt gccgccgtcc
      121 gcgaccacgc ctttgacgaa ggtggcagct tccgagtaca tcggctgcgt cttctcgagg
      181 ttgatgatgt ggatcttgtt tcgttcgccg aaaatgaacg gagccatctt cgggttccag
      241 aagcgggttt ggtgtccgaa atggacgccc gcttccagca tttgtcgcat tgacacgccg
      301 gacatcaaat ttctcctggt gggttaagcc tccgcgccgc ccgggtggat atccttgccc
      361 ctcttccatt gaagagggcc ctttggacac ccatccaccc gttatgccgt tgtctggaga
      421 gacccttgcc tatttagaag acaaggcggc aggttgcgcg tgtggtttaa gtgagtttca
      481 ttgccaaaaa aggccgcgct ttataccatc ccttccccgt caaaacaatg ccttacgcag
      541 gtgcacccca tggactccct cgtgagccgt cacatcacca ttaagtcccc ggccgagcag
      601 gaaaaaatgc gggttgccgg acggctggcg gccgaagttc tggacatgat cggcgagcac
      661 gtcaaacccg gcgtgaccac cgaggaactc gatcggctgt gttttgaaca cattacgcag
      721 gtgcagaagt cgatcccggc gaacgtgaac taccgcggct tcccgaaaac catctgctcc
      781 tcggtgaacc acgtggtgtg ccacggcatc ccgaacgaca aggcgctcaa gtcgggcgac
      841 atcctgaata tcgacgtcac cgtgatccgc gacggattcc acggcgacac cagccgcatg
      901 tactacgtcg gcaaaccggc cctgcccgca cagcgcctcg ccgagagctg ctgggaagcc
      961 atgtggcgcg gcatcgaaaa ggtgaagccc ggcgcgcgcc tcggtgacat cggccatgcc
     1021 atccagactt tcgtggaaga tcgaaacttc tccgtggttc gcgagtactg cggccacggc
     1081 atcggccgcg tgtaccacga ggatccccag gtcctgcact atggcgagcc cggcacgggc
     1141 gatgaactca aggcaggcat gactttcacg atcgagccca tggtgaacgc cggcaaacgg
     1201 cacgtgcggt tgttaggcga cggctggacc gtggtgacca aggaccactc gctgtcggcg
     1261 cagtgggaac acacctgcct ggtgaccgag aacggcgtcg aagtcctgac gctcggcgag
     1321 ggcagcatgc gccccgcggc gtcgaactag atggcgtcgc tcgaagaagg catcgaggaa
     1381 accgaaacga cggtcctgcg cagcacgccc tggatgcggc tcgcgaatct tccggaacgg
     1441 ctcgcggagg cgaaattcgc gcccgatgcg ttccggctcg aactgaagga agcaaacgag
     1501 gagttagccg agcgattccg cgccgatgag tcggtggaag tcctggtgca cgcgcgcgcc
     1561 gagctcatcg acgtcatcct gcgggaagtg tggcgctcgc agctgcccgc gggctacgaa
     1621 agctggacgc tggcggccgt cggcggttac gggcgcggcg aactgcatcc gcactccgat
     1681 gtcgacatcc tgatcctggt gccggccacg cccgacgatg ccggccgtgg cgtggtcgaa
     1741 cgcgtggtca cgttcctctg ggacatcaac ctcgaagtcg gccacagcgt gcgcaccgtc
     1801 gcgcaatgcg ccgaagagag cgtcgccgat gtcggcgtga tgaccacgct ggtcgaggcg
     1861 cgcctgctcg ccggcaacgc cgagttgtta gccgagatgc gcgaggcggt ttccgccgac
     1921 cgcgtgtggt cgatcaagca gtacttcgaa gcgaaggtcg ccgagcagca ggatcgccat
     1981 ctcaaggcga acgacaccgc ctacaacctc gaacccaacg tgaagacggg ccccggcggc
     2041 ctgcgcgaca tccagaccat tgcctgggtg gccaaacgcc acttcggcgc gagctcgctg
     2101 gacgaactcc tcacgcacgg cttcctgtcg ttcaccgagt tgcgcaagct caagcaggcg
     2161 cagtcattcc tctggaaagt ccgcttcggc ctgcacatcg ttaccggccg cagggaagac
     2221 cgcctgctgt tcgatcacca gatcaagctc gcgcagatgt tcggctacga agacgcgtcg
     2281 tacacgctcg cggtcgagca gttca
//
LOCUS       DATDEU010000403         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1533847, whole genome shotgun sequence.
ACCESSION   DATDEU010000403 DATDEU010000000
VERSION     DATDEU010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533847"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VIV63_09590"
     CDS             <1..1212
                     /locus_tag="VIV63_09590"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY6124894.1"
                     /translation="IPFTPVTSVDAAFFQRFDSQLAELREQGLFKSERVIASPQAGQI
                     RANDAEVINLCANNYLGLASHPAIREAAHRAIDRYGYGMASVRFICGTQTVHKQLEAA
                     LSRFFGAEDTILYSSCWDANGGLFETVLGEQDAIISDSLNHASIIDGIRLCKARRLRY
                     ANRNLAELEQHLKDTQDCRTRLIATDGVFSMDGSIAPLKEICDLADQYGALVMVDDSH
                     ATGFMGTGGRGSIEHCGVMGRVDFLTGTFGKALGGGSGGYTSGRANAIAWLRQRSRPY
                     LFSNSIPPVVAAVTLRALDMVENMPELRGTLFAHAKKFRAGMAAAGFKLLPGEHPIIP
                     VMIGDAALAARFADRLLAEGVYVIGFSFPVVPKGEARIRTQMSAGLTDAQLDRALAAF
                     IKVGRELELIK"
     gene            1209..>2050
                     /gene="tdh"
                     /locus_tag="VIV63_09595"
     CDS             1209..>2050
                     /gene="tdh"
                     /locus_tag="VIV63_09595"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY6124895.1"
                     /translation="MKALVKSEAKPGIWMKDIEVPKVGPNDVLIKIHKTAICGTDMHI
                     FNWDAWAQKTIPVPMAVGHEYFGRIVDMGSEVRGFAVGDRVSGEGHLTCGYCRNCRAG
                     RRHLCIHTVGVGVDRQGCFAEYLAIPASNAFKLPDAVTDDIASILDPFGNATHTALAF
                     NMVGEDVLITGAGPIGIMAVAIAKRVGARHVVITDVNDFRLGLAQKMGATRAINVTRQ
                     KLDDTMKELGMQEGFDVGLEMSGNPVAFRDLLRTMHHGGSVALLGIPPGETAIDWNQV
                     IFKGL"
BASE COUNT          372 a          708 c          630 g          340 t
ORIGIN      
        1 attcccttta ccccggtgac gtcagtggac gcagcctttt ttcaacgatt cgacagccag
       61 ctcgccgaac ttcgcgagca ggggctgttc aagtccgaac gcgtgatcgc cagcccccag
      121 gccggccaga tccgcgccaa cgacgccgag gtcatcaacc tctgcgctaa caactacctg
      181 ggcctcgcca gccacccggc catccgcgaa gcggcgcacc gcgccatcga ccggtacggt
      241 tacggcatgg cctcggtgcg cttcatctgc ggtacccaga cggtgcacaa gcagctggaa
      301 gcggcgctgt cacgtttctt cggcgccgag gacaccatcc tgtactcgtc ctgctgggac
      361 gcgaacggcg gattgttcga gacggtgctc ggcgagcagg acgccatcat ctcggactcg
      421 ctcaaccacg ccagcatcat cgacggcatc aggctctgca aggcgcggcg cctgcgttac
      481 gccaaccgca acctggccga actggaacag cacctgaagg acactcagga ttgccgcacg
      541 cggctcatcg ccaccgacgg cgtgttctcc atggacggca gcatcgcgcc cctgaaggag
      601 atctgcgacc tcgcggacca gtacggcgcc ctggtcatgg tcgacgattc acacgccacc
      661 ggcttcatgg gaaccggcgg ccgcggctcg atcgaacact gtggcgtgat gggtcgcgtg
      721 gacttcctta ccggcacctt cggcaaggcc ctgggcggcg gcagcggcgg gtatacctcg
      781 ggccgcgcca atgccatcgc ctggctgcgg caacgttccc gcccctatct gttctccaac
      841 agcatcccgc ccgtggtcgc ggccgtcacg ctccgcgcgc tcgacatggt ggagaacatg
      901 ccggaactgc gcggcacgct gttcgcacac gcgaagaagt tccgcgccgg catggccgcc
      961 gccggtttca aactactgcc cggtgaacat cccatcattc ccgtgatgat cggcgacgcc
     1021 gcgctggcgg cgcgattcgc cgaccggctg ctggccgaag gcgtgtacgt catcggcttc
     1081 tcgtttcccg tcgtgccgaa aggcgaggcc cgcatccgca cgcagatgtc ggccggtttg
     1141 acggacgcac aactcgatcg cgcgctggcc gccttcatca aggtgggccg cgaactggag
     1201 ctcatcaaat gaaggcgctg gtcaagagcg aagccaaacc cggcatctgg atgaaggaca
     1261 tcgaagtccc caaggtcggg cccaacgatg tgttgatcaa gatccacaag accgccatct
     1321 gcggtacgga catgcacatc ttcaattggg acgcgtgggc gcagaaaacc attcccgttc
     1381 ccatggccgt gggtcacgag tacttcggac gcatcgtcga catgggcagc gaggtgcgcg
     1441 gcttcgcggt cggcgaccgg gtgtcgggtg aaggccatct cacctgtggc tactgccgca
     1501 actgccgcgc cggccggcgt cacctgtgca tccatacggt gggcgtgggc gtggatcgcc
     1561 agggctgttt cgccgagtac ctggccattc cggccagcaa cgccttcaaa ctccccgacg
     1621 ctgtcaccga cgacatcgcc agcatcctcg atcccttcgg caatgccacg cacaccgccc
     1681 tcgccttcaa catggtgggc gaagacgtgc tgatcaccgg cgccggcccc atcggcatca
     1741 tggccgtggc catcgccaag cgcgtgggcg cgcgccacgt ggtcatcacc gacgtcaacg
     1801 atttccgcct gggcctcgcg cagaagatgg gcgccacgcg cgccatcaac gtcacgcggc
     1861 agaagctcga cgacacgatg aaggaactgg gcatgcagga aggcttcgac gtcggcctcg
     1921 agatgtcggg caaccccgtc gcgtttcgcg acctgctgcg caccatgcat cacggcggca
     1981 gcgtggcctt gttaggcata ccgccgggcg agacggccat cgactggaac caggtgatct
     2041 tcaagggcct
//
LOCUS       DATDEU010000404         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1097518, whole genome shotgun sequence.
ACCESSION   DATDEU010000404 DATDEU010000000
VERSION     DATDEU010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097518"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /gene="lptC"
                     /locus_tag="VIV63_09600"
     CDS             complement(<1..575)
                     /gene="lptC"
                     /locus_tag="VIV63_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY6124896.1"
                     /translation="MKRPSRPFAVVAVIAAVGIAYFLGRPGRDDGSDDVVSGSTADPG
                     YAAREATVIETGYDGRERYRLNASVIRQQTESGVIELEKLAMDYHPDRQNKLPGESAP
                     ASIDAGEVWHLTSDHGLVRADGDDVQLTGNVRVTGPAPGTGEPLSLTTDTLRINTPTE
                     YIETYSKVILKWSGYELRALGLKADLKAGTLR"
     gene            complement(572..1078)
                     /locus_tag="VIV63_09605"
     CDS             complement(572..1078)
                     /locus_tag="VIV63_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY6124897.1"
                     /translation="MIDARIQPLLARVRLLVLDVDGVLTDGRLFYGPKGELLKAFHVR
                     DGHGIKAVAAAGITVAIISGRKSAAVVRRAKELGIRHVTQGASDKLSALRKLAKARRV
                     TLEECACVGDDTPDAPILAAAGVGVAVADAHQDALAVADVVTTRPGGHGAVREVCDWL
                     VAARKAAP"
     gene            complement(1075..2061)
                     /locus_tag="VIV63_09610"
     CDS             complement(1075..2061)
                     /locus_tag="VIV63_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY6124898.1"
                     /translation="MNAQLKADPASLIAAGRRALEIEGRAIGALVPRLDATFARACSI
                     CLDCRGRVIVSGMGKSGHIAGKIAATLASTGTPSFFMHAAEASHGDIGMITRDDVLLA
                     ISNSGETSEVLLLIPHLSRLGVPIISMTGNPASSLARASTVHLDVSVAEEACPLNLAP
                     TASTTATLAIGDALAVAMLEARGFTSQDFARSHPGGALGRKLLMHVADVMRTGEDLPV
                     IGPEKSLADGLVVMSKKGLGMCVVVEAGQMLGVFTDGDLRRVLDRESNVHKTSMREVM
                     TSPGKRVAATELAAEAAHLMEKHRITALPVVDAQGVLVGALNVHDLLRAGVL"
     gene            2091..2351
                     /locus_tag="VIV63_09615"
     CDS             2091..2351
                     /locus_tag="VIV63_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY6124899.1"
                     /translation="MNSSVMTPERIADLIRTGLPGAAVRVESDDNTHFASQIVAAQFA
                     GLRPIARHQLVYKALGALMGREIHALSIEALTPEEFHLRSGA"
     gene            2375..3634
                     /gene="murA"
                     /locus_tag="VIV63_09620"
     CDS             2375..3634
                     /gene="murA"
                     /locus_tag="VIV63_09620"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY6124900.1"
                     /translation="MDKLQINGGIALEGEVRISGAKNATLPIIAAALLADGPVTIRNV
                     PHLQDVTTMIELLGRMGVSVTIDEKMQIEIDGTTTRECFAPYDLVKTMRASILVLGPL
                     LARFGKADVSLPGGCAIGARPVNIHVAGLQAMGAEINIDSGYIRARAGRLKGARLNLD
                     TVTVTGTENLMMAATLADGETIIENAAREPEVVDLANFLIAMGAKIKGAGTDKIAIQG
                     VERLRATTYEVLPDRIEGGTYLVAGAITSGMVRIKNTAPEHLDAVTAKLMEAGAKVGI
                     GPNWIEVDMRGRRPKSVDIRTAPYPAFPTDMQAQFAALNTIADGVGTIVETIFENRFM
                     HMLEMRRLGAEIRLEGNTAIIKGVPKLTAAPVMATDLRASASLVLAGLVAEGRTEIER
                     IYHIDRGYEALEEKLAQIGARIRRVPN"
BASE COUNT          700 a         1269 c         1316 g          645 t
ORIGIN      
        1 cgcagcgtgc cggccttcaa atcggctttc agaccgagcg cacggagctc gtatcccgac
       61 cacttgagta tcaccttcga gtacgtctcg atgtactcgg tcggtgtgtt gattcgcagg
      121 gtgtcggtgg tcaatgaaag cggttcgcca gtgccgggcg caggcccggt gacacgcacg
      181 ttgccggtga gctgaacgtc gtcaccgtcg gcacgcacca gcccgtgatc ggaggtcagg
      241 tgccagactt cgcctgcgtc gatggaggcg ggcgccgact cgccgggcaa cttgttctgc
      301 cggtccgggt gatagtccat cgcgagcttc tcgagctcga tgactcccga ttcggtctgc
      361 tggcggatca cgctggcgtt gaggcgataa cgttcgcggc cgtcgtaccc ggtttcgatg
      421 acggtagctt cgcgcgccgc ataaccggga tcggcggtgg agcccgacac gacatcgtca
      481 ctgccgtcat cgcggccggg acgtccgaga aaataggcga tgcccaccgc ggcgatcacc
      541 gcgacaaccg cgaacggacg cgaggggcgt ttcatggcgc ggccttgcgc gccgccacca
      601 gccagtcgca aacctcgcga acggcgccgt gtccgccggg acgcgtcgtg accacgtcgg
      661 cgaccgcgag cgcatcctga tgcgcatcgg ccaccgcgac gccgacgccc gcggcggcga
      721 ggatcggcgc gtcaggtgtg tcgtcgccga cgcaggcgca ttcttcgagc gtcacgcggc
      781 gcgccttcgc gagtttgcgc aacgcgctga gcttgtcgct cgcgccctgg gtgacgtggc
      841 ggatgccgag ttctttcgcg cggcgcacga ccgcggccga cttgcggccc gagatgatgg
      901 ccaccgtgat acccgcggcc gccacggcct tgatgccgtg accgtcgcgc acatgaaacg
      961 ccttgagcaa ctcgcccttc gggccgtaga acaggcgccc gtcggtgagc acgccgtcca
     1021 catcgagaac cagcaatcgc acgcgcgcga gcagcggctg gattcgcgcg tcgatcacag
     1081 caccccggcg cgaagcaggt cgtgcacgtt gagagcgccg acgagcacgc cttgcgcgtc
     1141 gaccacgggg agtgcggtga tgcggtgttt ctccatgaga tgggcggctt cggctgcgag
     1201 ttcggtcgcg gccacgcgtt tgccgggaga ggtcatgacc tcgcgcatcg aggtcttgtg
     1261 cacattggat tcgcggtcca gcacgcgccg caggtcgccg tcggtgaaga cgccgagcat
     1321 ctggccggcc tcgaccacca cgcacatgcc gagacctttc ttcgacatga cgaccaggcc
     1381 gtccgcgagc gatttctccg ggccgatgac cggcaggtct tctccggtgc gcatcacgtc
     1441 ggcgacgtgc atcagcagct tgcggccgag cgcgccgccg ggatgggaac gtgcgaaatc
     1501 ctgtgaggtg aagccgcgcg cttcgagcat ggccacggcg agtgcgtcac cgatggcgag
     1561 cgtggcggtc gtgctggccg tgggcgcgag attgaggggg caggcttcct ccgcgacgct
     1621 gacgtcgagg tggacggtcg aggcacgcgc cagcgacgag gccggattcc cggtcatgga
     1681 gatgatgggc acgccgaggc gggacaggtg cggaatgagc agcagcactt cagaggtctc
     1741 gccggagttg gagatggcga gcaacacgtc gtcgcgcgtg atcatgccga tgtcgccatg
     1801 gctggcctcg gccgcatgca tgaagaagga cggcgtgccg gtggaggcga gggtggccgc
     1861 gattttcccg gcgatgtgcc cggacttgcc catgccactg acgatgaccc ggccccggca
     1921 gtcgaggcag atgctgcagg cgcgcgcgaa cgttgcgtcg agccgcggca ccagcgcccc
     1981 gatggcccgg ccctcgatct cgagagcccg gcgtcccgcg gcgatcagcg aagccggatc
     2041 ggccttgagc tgggcattca tgcagcgatc ataacgctta agataacgcg atgaactcga
     2101 gtgtcatgac cccggagcgt attgccgacc tcattcgcac cggactgccg ggcgccgcgg
     2161 tccgcgtgga atccgacgac aacacgcatt tcgcctcaca gatcgtcgcg gcgcagttcg
     2221 cgggcctgcg gcccatcgcc cgccaccagc tcgtgtacaa ggccctgggt gcgctcatgg
     2281 gccgcgagat tcatgccctg tcgatcgagg ctttgacgcc tgaagagttc catctgcgct
     2341 ctggcgcctg accccttccg agagagaaca atccgtggac aaactgcaga tcaatggtgg
     2401 cattgcgctc gagggcgaag tgcgtatttc cggtgccaag aacgcgactt tgccgatcat
     2461 cgccgcggcg ctgctggccg atgggccggt gaccatccgt aatgtcccgc atctgcagga
     2521 cgtcaccacc atgatcgagt tgttagggcg catgggcgtc tcggtcacca tcgacgagaa
     2581 gatgcagatc gagatcgatg gcaccaccac gcgcgaatgt ttcgcgccgt acgatctcgt
     2641 gaagaccatg cgcgcgtcca tcctcgtgct gggacctctg ctggcgcggt tcggcaaggc
     2701 cgacgtgtcg ctgccgggag gctgtgccat cggcgcgcgg ccggtgaaca tccacgttgc
     2761 gggccttcag gcgatgggcg ccgaaatcaa catcgacagc ggctacatcc gcgcgcgcgc
     2821 cggacgcctc aagggtgcgc gcctgaacct ggacaccgtc accgtcacgg gcaccgagaa
     2881 cctcatgatg gcggccacgc tcgccgacgg tgaaaccatc atcgaaaacg ccgcgcgcga
     2941 gccggaagtg gtcgatctcg cgaatttcct catcgccatg ggcgcgaaga tcaagggtgc
     3001 gggcaccgac aagatcgcga tccagggtgt ggagaggctg cgcgcgacca cctacgaagt
     3061 gctgcccgat cgcatcgaag gcggcaccta tctggttgcg ggcgccatca cctcgggcat
     3121 ggtgcgaatc aagaacacgg ctcccgaaca cctcgacgcc gtgactgcaa aactcatgga
     3181 agccggcgcc aaggtcggca tcggacccaa ctggatcgaa gtggacatgc gcgggcgccg
     3241 cccgaagtcc gtggacatcc gcaccgcgcc atatccggcg tttccgaccg acatgcaggc
     3301 gcaattcgcg gcgctcaaca cgatcgccga cggtgtcggc acgatcgtcg agaccatctt
     3361 cgaaaaccgg ttcatgcaca tgctggagat gcggcgcctg ggcgccgaga tccgcctcga
     3421 gggcaacacg gccatcatca agggcgtgcc caaactcacg gccgccccgg tcatggcgac
     3481 cgacctgcgg gcgtcggcca gcctggtgct ggcgggtctc gtggccgagg gccggaccga
     3541 aatcgagcgc atttatcaca tcgatcgcgg ctacgaggcg ctcgaggaaa agctggcgca
     3601 gatcggcgcc cggatccggc gcgtacctaa ctaggagaca aaggggacaa tcctcgtttt
     3661 gtactgattt taaaggcccg cggcgtgtct gagagccacg tccctgtttg ccgcaggaaa
     3721 caggaatgtc ccgccgcacc atttgtgatc caggtcgcga tccggccgta agcgggagat
     3781 tcccttgcgc cgtatgtgac tcagtgccga tcaacggcaa cgcactgact taatactggc
     3841 cctcactgtc gttccccgga gacgggccag gaaatggccg gcgccgcgag gaagagccat
     3901 cgtcgcaaaa ctagttcgat gagaggtcga
//
LOCUS       DATDEU010000405         2404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1242128, whole genome shotgun sequence.
ACCESSION   DATDEU010000405 DATDEU010000000
VERSION     DATDEU010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242128"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VIV63_09625"
     CDS             complement(<1..215)
                     /locus_tag="VIV63_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY6124901.1"
                     /translation="MANASLAPVLLVSMPQMNDANFSKTVVLLAEYGAHGAFGLVLNR
                     RMDEPAEAIITADDPLDIHPEMHLYLGG"
     gene            complement(235..651)
                     /locus_tag="VIV63_09630"
     CDS             complement(235..651)
                     /locus_tag="VIV63_09630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY6124902.1"
                     /translation="MVWHELTRGIVMPLHCDLYGHMNVRHYAAFFDDAGWHMLAKAGV
                     SLDELRAQGLGSVVASLTIDFHHEMTAGQLFLVSGAFTRVGARSFGNEMRLYESESMT
                     HCATQKTVEVCFDTRARASASWPDPVRSKIRQAIGE"
     gene            complement(656..1360)
                     /locus_tag="VIV63_09635"
     CDS             complement(656..1360)
                     /locus_tag="VIV63_09635"
                     /inference="COORDINATES: protein motif:HMM:NF006093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEY6124903.1"
                     /translation="MNSPDESIQVLTAVTTATVTTILLKKGLRNVWIRGAFPLAPGAN
                     RVVGRAFTVRFIPAREDLATPASWSSPRSTRAAIEDMPAGCIAVVDANGVTDAGFWGD
                     ILCARMAQRGVAALVSDGAVRDVAGVLSTGLPVWAAGVAAPPSVAGLTFVDWQQPIGC
                     GGVAVFPGDVIVADQDGAVVIPAAMVEEVTALAVEQERLEGWIMDEIKRGVALPGLYP
                     ANDETKARYEAWKAKG"
     gene            complement(1386..1862)
                     /locus_tag="VIV63_09640"
     CDS             complement(1386..1862)
                     /locus_tag="VIV63_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124904.1"
                     /translation="MAKLMFFCGKMASGKSTLAKQLAEREDAVLLVQDQLLDTLFPGL
                     IVNVASYLEYAGRINKMAAPHVAAILSKGVSVVLDFPANTRNQRAWFRRILDESGVEH
                     ELHFLNTSDAVCKAQLRARSAHLPPGTKWTTDEDFELIASHFVAPALDEGFNVIVH"
     gene            1834..>2404
                     /locus_tag="VIV63_09645"
     CDS             1834..>2404
                     /locus_tag="VIV63_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124905.1"
                     /translation="MPQKNISFAIRATPLSSFTLRSLSRQCCYHAHHPTMTDVDEELS
                     AKKLRKLEKNSDTRALDPWERYRAMIDLYEAFFDMNELADRKTRFALVMMGALNALNL
                     ILGSQRMFGDLTGGVRPLVAAYFMTYAVLSLYFFIQAITTLRPRISMFPQGGAPQDTA
                     RAGLRFIGDVVSAGSDDYYQRWTKVEVGEL"
BASE COUNT          436 a          791 c          744 g          433 t
ORIGIN      
        1 ccgcccagat agaggtgcat ctccgggtgg atgtcgaggg ggtcgtcggc cgtgatgatc
       61 gcctcggccg gctcgtccat gcggcgattc aacaccagcc cgaacgcgcc gtgggcgccg
      121 tactccgcga gcagcactac cgtttttgaa aaattcgcat cgttcatctg cggcatcgac
      181 accagcagga ccggggcgag ggaggcgttc gccatggatc gatattactg cgggctactc
      241 cccgattgcc tgccggattt tcgatcggac gggatccggc caggacgccg aggcgcgcgc
      301 cctggtgtcg aagcacacct ccacggtctt ctgggtggca cagtgcgtca tcgattcgct
      361 ctcgtagagg cgcatttcgt tgccgaaact cctggcgcca acgcgcgtga aggcgccgct
      421 caccaggaac aactggcccg ccgtcatctc gtggtgaaag tcgatggtga gcgaagcgac
      481 cacgctgccc agcccttgtg cccgcagctc gtcaagcgac accccggctt tcgccagcat
      541 gtgccagccc gcgtcgtcga agaacgccgc gtagtggcgg acgttcatgt gtccatacaa
      601 gtcgcaatgc agcggcatca cgatgccgcg ggtgagctcg tgccacacca tcggtctagc
      661 ctttcgcctt ccacgcctcg tagcgcgcct tggtctcgtc gttggccgga tacaaccctg
      721 gcagcgcgac accgcgcttg atctcgtcca tgatccagcc ttcgaggcgc tcctgctcga
      781 cggcaagcgc cgtgacctct tcgaccatgg ccgctggaat cacgacggcg ccatcctgat
      841 cggcgacgat cacgtcgccc ggaaacaccg cgacaccgcc gcagccgatc ggttgttgcc
      901 agtcgacgaa cgtcaatccc gccaccgacg gtggcgcagc gacgccggcg gcccacaccg
      961 gcaacccggt cgacagtact ccggcgacat cacgcacggc gccgtcgctg acgagcgcgg
     1021 cgacaccgcg ttgcgccatg cgcgcgcaga gaatgtctcc ccagaaaccc gcatccgtga
     1081 cgccgttcgc atccaccacc gcgatgcatc cagccggcat atcttcgatc gcagcacgcg
     1141 tcgacctggg cgagctccag gatgcgggcg tggcgagatc ctcgcgcgcc gggatgaagc
     1201 ggaccgtgaa agcgcggccg acgacgcgat tggcgccggg cgcgagcggg aaggcgccgc
     1261 ggatccagac gttgcgcagg cccttcttca gcagaatggt ggttacggtc gccgtcgtca
     1321 cggccgtcaa tacctggatc gattcgtcgg gcgaattcat ggtccgcgat tgtagtccga
     1381 accaatcaat ggacgatgac gttgaatcct tcgtcgagcg ccggcgcgac gaagtgcgac
     1441 gcaatcagct cgaagtcctc gtcggtggtc catttcgtgc ccggcggtaa atgggcgctg
     1501 cgcgccctga gctgcgcctt gcagacggcg tcagaggtgt tcaggaagtg cagttcgtgt
     1561 tcgaccccgg actcgtcgag gatgcggcga aaccacgcgc gttgattgcg cgtattggcc
     1621 ggaaagtcga gcacgacgct gacccccttc gagagaatgg cggcaacgtg tggcgcggcc
     1681 atcttgttga tacgaccggc atattccagg tagctcgcga cgttgacgat caagccgggg
     1741 aacagggtat ccagcagctg atcctggacc agcagcaccg catcctcgcg ctcagccagt
     1801 tgctttgcga gcgtcgactt acccgacgcc atcttgccgc agaagaacat cagtttcgcc
     1861 atccgagcca caccgctttc atcctttacc ctgcggtcgc tgtcgcgcca gtgctgctat
     1921 catgcgcacc acccgaccat gaccgacgtt gacgaggaac tatccgccaa gaagcttcgc
     1981 aagctggaaa agaattccga cacccgggcg ctcgatccct gggaacgcta ccgcgcgatg
     2041 atcgacctct atgaggcgtt cttcgacatg aacgagctgg cggatcgaaa gacgcggttc
     2101 gcgctggtga tgatgggtgc gctcaacgcg ctcaacctga ttcttggctc gcaacgcatg
     2161 ttcggcgatc tcaccggggg cgtgcggccg ttggtcgccg cctatttcat gacctacgcg
     2221 gtcctctcgc tatacttctt tatccaggcg atcaccacgc tgcggccgcg catctcgatg
     2281 tttccgcagg gcggcgctcc gcaggacacg gcgcgagcgg gattgcggtt catcggcgac
     2341 gtggtcagcg ccggcagcga cgactactac cagcggtgga cgaaggttga agtcggcgaa
     2401 ctgt
//
LOCUS       DATDEU010000406        12425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_806623, whole genome shotgun sequence.
ACCESSION   DATDEU010000406 DATDEU010000000
VERSION     DATDEU010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806623"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VIV63_09650"
     CDS             <1..265
                     /locus_tag="VIV63_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124906.1"
                     /translation="PQVLSLELQRSLDYYESHFDETPITELFIAPAGIRANLLAAELG
                     ISTGLRISMFNIHDLVDVSFSAEIPGGWLPCMAIGAAMRAPKG"
     gene            271..906
                     /locus_tag="VIV63_09655"
     CDS             271..906
                     /locus_tag="VIV63_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124907.1"
                     /translation="MQEVNLYQPVAKGVRGALSASSSRNTLLLVGVTLFGFWGFAYWQ
                     VGRLQDATEVVRNQALAQAAMSAAQGPQLGGLSDEELAALLAKLSSDVDVKTRALALL
                     NAEAGRPAAGFAARLRAFGTRHVDGIWLDRLTFGSDARSVTVSGSTLSPGTVPRYLRS
                     LAADPALEGGQIDEFVIEKPGDPKTGGSGRLSFHAGHRGLQLPETGDREKS"
     gene            906..1532
                     /locus_tag="VIV63_09660"
     CDS             906..1532
                     /locus_tag="VIV63_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124908.1"
                     /translation="MDTGAMQKRFEALTLRERAFLMLAVLGSLVAGWDSLMMEPLRQT
                     KINLAAELAGATAFGGLPQSTDASDPRQLSISQAGELQTQMQNIDAQLASSTSSFVTS
                     QRMIEVLHDVLNAQGRLDLISIRNLPVTALIPAAEPSAPAQPPYVHAIEIVIDGEYAD
                     ILDYLGALEALPWKFRWNTFDLSTAGYPRNRVRIELSTLSLDSTWLGV"
     gene            1538..1900
                     /locus_tag="VIV63_09665"
     CDS             1538..1900
                     /locus_tag="VIV63_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124909.1"
                     /translation="MRRRFRLVHGTCLALLLSSAANAQAVRDPMRPPGTAAATSRPRP
                     AATLKLEGVISGTERVAIVSGRLVRAGDEIAGVRILEVLNDGVRFSRGGQIQSLLLPG
                     VRALGTVRVASSPEARKP"
     gene            1897..3450
                     /gene="mshL"
                     /locus_tag="VIV63_09670"
     CDS             1897..3450
                     /gene="mshL"
                     /locus_tag="VIV63_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HEY6124910.1"
                     /translation="MKRLIRMLPLTLLALGAVASAAETDTRPRYDLQVEDAPARAFFE
                     GLVVGTPVNMLVHPDVKGRVSLSLKQVTLEEALEATRDLYGYDYRPMANGYVVSAPAA
                     QTRVFQLNYLDLQRVGVSRTRVSSGQVTQGSSSASAGQGGTDDEKTQTSSTTGTAVLT
                     RNESDFWKGIEADIGALVSGDDKNGVVINRQSGVVVVRAFPDELRAVNDYLRKTQSTI
                     TRQVVLEAKIIEVELNAGFQAGINWTALVTKGSKTAAFGQSSPFGGFDGNPFSPQGTP
                     IPVTPGNPVVGQVISSLGSAFTFAFDSTDFSAFIELLDAQGRTRVLSSPRVSTLHNQK
                     AIIKAGTDEFFVTGVSSDTTTGESTTTSLDVELTPFFSGVALDVTPQISDDGSVLMHI
                     HPTVSDVQDQNKRISFGGSTSDLPLAVSQIRESDSVVRAHNGQVIVIGGLMREMRKRD
                     DYKTPFLGDVPGLGNLFKSKRDLSNTVELVLLLRPVVVADADWENMVRESTDRAAALS
                     HKGDVEGTR"
     gene            3447..5234
                     /locus_tag="VIV63_09675"
     CDS             3447..5234
                     /locus_tag="VIV63_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY6124911.1"
                     /translation="MNEPRRKKIRLGELLMEQKVINEAQLNQALVEQKKTGRKLGRVL
                     TDLGILQEQQLNEALARHLQIPFVDLKQLTLEPATVRLLPEALARRFRALVLQSDARG
                     LTVGMADPTDLFVYDELVARLKQPVRIALVREAELLRTMDIVYRRTEQIASIALEVRE
                     ELRAADIDISQLAIDEGSSDAPIIRLIQTIFHDAVNARASDVHIEPGEGLLRVRLRVD
                     GVLQEQTIEGRGVASALVTRLKLMSSLDISEKRLPQDGRFSVKVKDRSIDVRMATMPV
                     QFGESIVLRLLDQSNSLMTLEQLGMPAGMLAKFRSLIERPAGMMLVTGPTGSGKTTTL
                     YSALSYLNRSDTKILTAEDPVEYRLDRVSQVQVNGKIGLDFARILRTMLRADPDVILV
                     GEMRDKETAEIGLRAAITGHMVFSTLHTLTAAATVNRLLDMGVAGYMIATALHGIVAQ
                     RLVRQVCPDCAQPAEPDDHQLAWLHAQIGTAAANGMEFRVGAGCTYCNLSGYRGRAAI
                     YELIEVDRALADAIRRADSQGFADAARQQVGYTTLTRSAIDMAAAGKTTLGEVIATTS
                     GLDDPVQDSRRAAPSQEELTDALLSAQTG"
     gene            5248..6480
                     /locus_tag="VIV63_09680"
     CDS             5248..6480
                     /locus_tag="VIV63_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY6124912.1"
                     /translation="MAEFSYKGRSSEGKLVNGRAAGDSVDQVAQRLIASGVIPLQIDA
                     LGVVAGSLNFEKLGRRLGLGRVGTTDLVMFSRQMYTITRAGIPLLRGLRGLIASTHNA
                     ILRETLQDILESLEGGRDLAASFARHPKIFPPLYVSIVSVGEATGTLDKSFQRLTEYL
                     AQEQKMYDNVKGAVRYPIIVMITIALAAAFLSTVVIPKFAPVFAQLKGELPLPTRLLL
                     GSSTIVRDYWMYSLGVVGLLVFGARRYLQTQDGRYKWDRFKLRIPVMGKLMYEAMLSR
                     LNRSLAISLAAGMPMTQTLAVIARSSGNLFVAEKIQILASSVERGESLHRAAVASGMF
                     SPLVLQMIAIGEETGALPELLDEAASYYEREVDHAVKNLSASIEPILIVAVGGMVLVL
                     ALGIFMPLWEVISKAGQG"
     gene            6608..7093
                     /locus_tag="VIV63_09685"
     CDS             6608..7093
                     /locus_tag="VIV63_09685"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6124913.1"
                     /translation="MRSRIQAGFTMVELIVVIVILGILAAVALPKFMGLEREARVAAV
                     KSMGGTLLSAANMAHGVAMARNVTNGNVNMEGSAVTFVNGYPSNATVWNLIQSSDGFT
                     RAANTFTKTGAPTANCWVRYNQATLGANGAVIAPTLTYNNVAATPANGTAIDASLRTA
                     C"
     gene            7129..7596
                     /locus_tag="VIV63_09690"
     CDS             7129..7596
                     /locus_tag="VIV63_09690"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY6124914.1"
                     /translation="MPRKECRGFTLIELIASITIIAILGAVAWPRLSSSSQPYAERGY
                     ADGVAASLRQARAVAIASGCDVRFTITAAGYNAFQRGAAANNHCAVAGAWVTPVQRGD
                     GQSLLAMAPAGAALAVNRQFVFGADGSVVGGAVNITIGPQVIDVNASGVAYGP"
     gene            7593..8036
                     /locus_tag="VIV63_09695"
     CDS             7593..8036
                     /locus_tag="VIV63_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY6124915.1"
                     /translation="MKLMRKQSRGMTLIELIVSITVIAIAGAALMSTLAYLNGTGNTS
                     MLQAQAQSIANAYLNEIVGKQFVADGVEATRAQYDDIFDYNNLDTPAASDERGNAAGN
                     FRVRVAIVPGALNGLPAADVRRIDVTVDYGNASQVIASGYRTRYP"
     gene            8033..8821
                     /locus_tag="VIV63_09700"
     CDS             8033..8821
                     /locus_tag="VIV63_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6124916.1"
                     /translation="MNARGFTLVELVIAIAIASIVMVFSILFMRAPIDAYEAHSRRTD
                     LVNNAASAWPRMEADLRNALPNSVRARRNGNVVVLEMLPVLGVARYKTSPSTVPITTA
                     GVYAGVTPAFLSVNTLNGNAYALAGSMAAATNFTNPAGAVTGEQQIGVNPAPAFTSDS
                     PRRNLYFVARPVTWLCDEGLGTLQRYSGYNIAALQTARDTAGELTGAGAAVEPVARGL
                     TSCVFDVTPPGSTTQSQTAAVHLTATRNGDSVTLLHSVRSEYVP"
     gene            8818..9234
                     /locus_tag="VIV63_09705"
     CDS             8818..9234
                     /locus_tag="VIV63_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124917.1"
                     /translation="MRRLPSRQRGMSMVVAIFLITVIATLAAFAVTVGTATREAENLQ
                     LQADRAMAAARAGMQWAAYRARVAQVCPAGAFNLTEGALRGFRVTVTCVANTGLHAEG
                     AVTYRVFDVRSFAQWSNFGAANYASRTIDARFNTAP"
     gene            complement(9254..9820)
                     /locus_tag="VIV63_09710"
     CDS             complement(9254..9820)
                     /locus_tag="VIV63_09710"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF014590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY6124918.1"
                     /translation="MQFPIPALLGLAYGISEAGLAYLKRSRDDSVDADDATLRILWIT
                     IVLAVTLGVLASTWLPAAAMRGGAGIAFFAGCTLFGLGLALRWYSIAYLGRFFTVNVA
                     IHSRHEIIDTGPYRFIRHPSYIGALLAFLGLALTLANWASLAIVMVPIALAFGWRMST
                     EESALANALGSPYINYMRRTKRLVPFVY"
     gene            complement(9917..10726)
                     /locus_tag="VIV63_09715"
     CDS             complement(9917..10726)
                     /locus_tag="VIV63_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY6124919.1"
                     /translation="MKSSTLIVYRGRAGDRNSRGTAGAQMLGALLARRYGLDVTYVST
                     PHAPLPTTAWDEQLAAARGELSALADALRAAIRDGHRAVAAIGRCAAGLATVPVIAEA
                     HPDACVVWFDAHGDSNLPSSNAVPYLGGMVLTGAAGHWDSGLGAGLKLSNVVLVGARD
                     LDPHEKELIGSGELKLVAPGPNLAARLAAAVGDRDVYVHIDCDVLEPGIVPIEYQVTG
                     GLSLEDLRACAAILAKRKVIGIEIAEFESEWLDGRPASPDRVVDAIAPLLD"
     gene            complement(10806..11120)
                     /locus_tag="VIV63_09720"
     CDS             complement(10806..11120)
                     /locus_tag="VIV63_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124920.1"
                     /translation="MTTKRSKYDPMATSRLSRWLVLRDMFHNVLDATELPAGSDLRGS
                     FADGLAALEREGWQSEGASGAMVFVRRGGERRLLSIHEHDPHEPLPTGHGVLSGRCAT
                     CE"
     gene            complement(11549..12196)
                     /locus_tag="VIV63_09725"
     CDS             complement(11549..12196)
                     /locus_tag="VIV63_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6124921.1"
                     /translation="MKIAAKVPARKISVILADDHPVVRDGLAAIVNQQPDMQVVAEAG
                     DGAEALDLYELHKPDVMVLDLRMPKMDGAAVVQKVLEKYPKACLLIMTTYDGDEDIFR
                     SLSQGAKGYLLKDAPRQEILSAIRAVADDRPYTSSGIAAKALQRMSKPSLTTRELDVL
                     QLVAEGRSNKDIARRLAITEGTAKTHVKAILGKLDAISRTEAVAVAHKRGLIRLS"
     gene            complement(12193..>12425)
                     /locus_tag="VIV63_09730"
     CDS             complement(12193..>12425)
                     /locus_tag="VIV63_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124922.1"
                     /translation="PTTVRIVLLEEQARWTMSVIDDGRGMTLSPELYAQQGYGLNNMR
                     ERAGAIGGVFHIDSKPGEGTRIIVNLPRPVAR"
BASE COUNT         2115 a         4048 c         4137 g         2125 t
ORIGIN      
        1 tccgcaggtg ctgtcgctcg aattgcagcg ctcgctcgat tactacgaga gccatttcga
       61 cgagacgccg atcaccgaac tgttcatcgc ccccgccggc atccgcgcca acctgttggc
      121 cgccgagctc ggcatctcga ccggcctgcg catcagcatg ttcaacatcc acgatctcgt
      181 ggacgtgtcc ttcagcgccg agatccccgg cggctggctg ccgtgtatgg ccatcggcgc
      241 cgccatgcgc gcgccgaagg gatgacagcc atgcaggaag taaaccttta ccagccggtt
      301 gcgaagggtg tgagaggcgc gctgagcgcg agctcgtcgc gcaacacgtt gctgctcgtc
      361 ggcgtcacgt tgtttggttt ttggggtttc gcgtactggc aggtcggccg actgcaggat
      421 gcgaccgaag tcgtgcgcaa tcaggctctg gcccaggccg cgatgtcggc ggctcaaggg
      481 ccgcagctcg gtggcttgtc ggacgaggag ttggcggcgc tgctggcgaa actctcgagc
      541 gatgtggacg tcaagacccg cgcgctggcg ctcctcaacg ccgaggcggg acgccctgcg
      601 gcggggttcg ccgcgcggct gcgtgcattc ggcacgcggc atgtagacgg catctggctc
      661 gaccgcctca cgttcggctc ggacgcccgg tcggtgaccg tctccgggtc gacgctttcg
      721 cccggcactg tgccgcgata tctgcgcagc ctcgccgcgg atccggcgct cgagggcggg
      781 cagatcgacg aattcgtcat cgagaagccg ggggatccga agacgggtgg cagtggccgc
      841 ctgagtttcc atgccgggca ccgtggtctg cagttgcccg aaaccggcga ccgggagaaa
      901 tcctgatgga caccggcgcg atgcagaaac gattcgaggc gctgacgctg cgtgagcgcg
      961 cattcctgat gctggccgtt ctcggctcgc tcgtggcggg ctgggattcg ctgatgatgg
     1021 aacccctgcg gcagaccaag atcaaccttg cggccgagct cgcgggcgcg acggcattcg
     1081 gcgggttgcc gcagagcacc gatgcgtccg atccccgcca gttgtcgatc tcccaggcag
     1141 gtgagctgca gacgcagatg cagaacatcg acgcgcagct cgcgagcagc acgagcagct
     1201 tcgtgacctc gcaacgcatg atcgaagtcc tgcacgacgt gctgaacgcg cagggccggc
     1261 tcgatctcat cagcatccgc aacctgccgg tcaccgcgct gatcccggcc gccgagcctt
     1321 ccgcgcccgc gcagccgccg tacgtgcacg ctatcgagat cgtcatcgac ggcgaatacg
     1381 cggacatcct ggactacctg ggcgcgctcg aagcgctgcc gtggaaattc cgctggaaca
     1441 cattcgacct gtccaccgcg ggttatccgc gcaaccgcgt gcgcatcgag ctgtccacgc
     1501 tcagcctcga ttccacctgg ctgggagtct gaaggccatg cgcagacgct tccgcctggt
     1561 tcacggcacc tgcctcgcgc tgctgctgag ctccgccgcg aatgcacagg cggtgcgcga
     1621 tccgatgcgt ccccccggca cggccgcggc gacgtcacgc ccgcggcctg ccgccacgct
     1681 caagctcgaa ggtgtcatct cgggcacgga acgcgtcgcc atcgtcagcg gacggttggt
     1741 gcgcgcgggc gacgagatcg ccggcgtccg cattctcgaa gtgctcaacg acggcgtgcg
     1801 cttttcgcgc ggcggccaga ttcaatccct cctgctgccg ggcgtacgtg cgctcggaac
     1861 cgtgcgggta gctagttctc ccgaggcaag aaagccatga aacgcctgat ccggatgctt
     1921 cccctgaccc tgctggcgct cggtgccgtg gccagcgccg cggaaacgga tacccgtccg
     1981 cgctacgacc tgcaagtcga ggacgcgccc gcgcgggcct tcttcgaagg cctggtggtc
     2041 ggtacgccgg tcaacatgct ggtgcatccc gacgtgaagg ggcgcgtgtc gctgtcgctc
     2101 aagcaggtga cgctcgagga agcgctggaa gccacgcgcg acctgtacgg ctacgactac
     2161 cgcccgatgg cgaatggtta cgtggtctcg gcgcccgcgg ctcagacccg cgtgttccag
     2221 ctcaactatc tcgacctgca gcgcgtgggc gtgtcgcgta cccgcgtgag ctcgggccag
     2281 gtcacgcagg gcagcagctc ggcaagcgcc gggcagggcg gaaccgacga cgaaaagacc
     2341 cagaccagca gcaccacggg aaccgcggtg ctcacgcgca acgaatcgga cttctggaaa
     2401 ggcatcgagg ccgacatcgg ggcgctggtg tcgggcgacg acaagaacgg cgtggtcatc
     2461 aatcgtcagt cgggcgtcgt ggtcgtgcgc gccttccccg acgagctgcg cgccgtcaat
     2521 gactacctgc gcaagacgca gtcgactatt acgcgccagg tggtgctcga ggcgaagatc
     2581 atcgaggtcg agctcaatgc cggattccag gccggcatca actggaccgc gctggtaacg
     2641 aagggcagca agaccgcggc cttcggccag agctcgccgt ttgggggttt cgacggcaat
     2701 ccgttttcgc cgcagggcac gccgatcccg gtgacgccgg gaaatccggt ggtggggcag
     2761 gtgatcagct cgctcggcag cgccttcacg ttcgcgttcg attccaccga cttcagcgcg
     2821 ttcatcgaac tactcgatgc gcagggccgc acacgcgtgt tgtcgagccc gcgcgtttcg
     2881 acgctgcaca accagaaggc catcatcaag gccggcaccg acgaattctt cgtgaccggc
     2941 gtgtcgagcg acacgaccac cggcgagtcg accacgactt cgctcgacgt ggagctgaca
     3001 ccgttcttct ccggcgtcgc gctcgacgtg acgccgcaga tcagcgacga cggttccgtg
     3061 ctgatgcaca tccatccgac ggtcagcgac gtgcaggacc agaacaagcg tatctcgttc
     3121 ggcggctcga ccagcgacct gccgctggcg gtcagccaga tccgcgaatc cgacagcgtc
     3181 gtgcgcgcgc acaacggcca ggtgatcgtg atcggcggcc tcatgcgcga gatgcgcaag
     3241 cgcgacgact acaagacgcc gttcctcggc gacgtccccg gcctcggcaa tctcttcaag
     3301 agcaagcgcg acctcagcaa caccgtggag ctggtgctgc tgctgcgccc ggtcgtcgtc
     3361 gccgacgccg attgggagaa catggttcgt gagtccacgg accgcgccgc ggcgctgagc
     3421 cacaagggag atgtcgaggg aacgcgatga acgagccgcg ccgcaagaag atccgcctgg
     3481 gtgagctgct catggagcag aaggtcatca atgaggcgca gctgaaccag gcgctggtcg
     3541 agcagaagaa gaccggccgc aagctcggcc gcgtgctcac ggatctcggc atcctccagg
     3601 aacagcagct caacgaagcg ctggcccgtc acctgcagat tcctttcgtc gacctcaagc
     3661 agctcacgct ggaaccggcg accgtgcgcc tgttgcccga agcgctggcg cgccgtttcc
     3721 gcgcgctggt gctgcagtcg gatgcgcgcg ggctcaccgt cggcatggcc gacccgaccg
     3781 acctgttcgt ctacgacgaa ctggtggcga gactcaagca gccggtgcgc atcgcgctgg
     3841 ttcgcgaggc ggagctgctg cgcacgatgg acatcgtgta ccgccgcacc gagcagatcg
     3901 cgtccatcgc gctcgaagtg cgcgaagagt tgcgggccgc cgatatcgac atttcccagc
     3961 tggcgatcga tgagggctcg tcggacgcgc cgatcatccg cctgatccag accatctttc
     4021 acgacgccgt caacgcgcgc gcgtccgacg tgcacataga gcccggcgaa ggtctgttgc
     4081 gggtgcggtt gcgcgtcgat ggcgtattgc aggagcagac catcgaaggc cgcggcgtgg
     4141 cgagcgcgct ggtcacccgc ctgaaactca tgagcagtct cgacatctcc gagaagcgcc
     4201 tgccgcagga cggccgcttc tccgtcaagg tgaaggaccg ttcgatcgac gtgcgcatgg
     4261 cgacgatgcc cgtccagttc ggcgagtcca tcgtgctgcg actgctcgat caatccaaca
     4321 gcctcatgac gctcgagcag ctcggcatgc cggccgggat gctggcgaag tttcgcagcc
     4381 tcatcgaacg tccggccggc atgatgctcg tcacggggcc caccggcagc ggcaagacca
     4441 cgacgctgta ttcggcgctg agctatctca atcgttccga cacgaagatc ctcacggccg
     4501 aggatccggt ggaataccgc ctggaccgcg tcagccaggt ccaggtcaac ggcaagatcg
     4561 gactcgactt cgcgcgcatc ctgcgcacca tgcttcgcgc cgaccccgat gtgatcctgg
     4621 tcggcgaaat gcgcgacaaa gagaccgccg agatcggtct gcgcgccgcc atcaccggcc
     4681 acatggtgtt ctcgacgctg catacgctca cggcggccgc gaccgtcaac cgcctgctcg
     4741 acatgggcgt ggccggatac atgatcgcca cggctttaca tggcatcgtg gcgcagcgcc
     4801 tggtacgcca ggtctgcccc gactgcgcac agccggcgga acccgatgat catcagctcg
     4861 cctggttgca cgcgcagatc ggcactgccg ccgccaatgg catggaattc cgcgtcggcg
     4921 cgggctgcac ctactgcaac ctctcgggtt atcgcggccg agcggctatc tacgagctca
     4981 tcgaagtgga tcgcgcgctc gctgacgcca tccgtcgcgc cgattcacaa gggttcgcgg
     5041 acgccgcgcg gcagcaggtg ggctacacca cgctcacgcg cagcgccatc gacatggcgg
     5101 cggcgggcaa gaccacgctc ggcgaagtca tcgccaccac cagcggcctc gatgacccgg
     5161 tgcaggactc acgccgcgcc gcgccgtcgc aggaggaact gaccgacgcg ctgctctccg
     5221 cgcaaaccgg gtgagccggg tcgagccatg gccgaattca gctacaaggg caggtcgagc
     5281 gagggcaagc tggtgaacgg ccgcgccgcg ggcgactccg tcgaccaggt ggcgcagcgc
     5341 ctcatcgcga gcggcgtcat tccgctgcag atcgacgccc tgggggtggt cgccggaagc
     5401 ctcaacttcg agaagttagg tcgaaggctt ggcctaggcc gcgtcggcac cacggacctc
     5461 gtgatgttct cgcggcagat gtacacgatc acgcgcgcgg ggattccgct gctgcggggt
     5521 ttgcgcggcc tgatcgcctc gacgcacaac gccatcctgc gcgagacgct gcaggatatt
     5581 ctcgagagcc tcgagggcgg ccgcgacctc gccgcgagct tcgcgcgcca tccgaagatt
     5641 ttcccgccgc tctacgtgag catcgtcagc gtcggcgaag ccacgggcac gctcgacaag
     5701 tcgttccagc gcctcaccga atacctggcg caggaacaga agatgtacga caacgtcaag
     5761 ggcgcggtgc gatatcccat catcgtgatg atcaccatcg cgctcgcggc cgcgttcctg
     5821 tcgacggtcg tgatcccgaa attcgcgccg gtgttcgcgc agctcaaggg agagctgccg
     5881 ctgccaaccc gcctgctgct cgggtcctcg acgatcgtgc gcgactactg gatgtattcg
     5941 ctcggagtcg tcggcctgtt ggtgttcggg gcacgtcgat atctgcagac ccaggacgga
     6001 cgctacaagt gggaccggtt caagctgcgc atcccggtga tgggcaagct catgtacgaa
     6061 gcgatgctgt cgcgtctcaa ccggtcactc gccatctcgc tcgccgcggg aatgcccatg
     6121 acgcagacgc tggcggtgat cgcgcgatcg tcgggcaacc tgttcgtggc cgagaagatc
     6181 cagatcctcg cgagcagcgt cgaacgcggc gagtcgctgc accgcgcggc ggtcgcaagc
     6241 ggaatgtttt cgccgctcgt gctccagatg atcgcgatcg gcgaggagac cggcgctttg
     6301 cccgagttgc tcgatgaagc ggcgagctac tacgagcgcg aagtcgacca tgcggtgaag
     6361 aacctgagcg cctcgatcga accgatcctg atcgtcgccg tgggcggaat ggtgctggtg
     6421 ctcgcgctcg gcatcttcat gccgctgtgg gaagtcatca gcaaggccgg ccaggggtga
     6481 gcgcgctgca ttatgtctgt aggactgtta atcgccgcac gcttcaaggg tttcgagtaa
     6541 aggccgggcc tttcgcggcc gatacagcgc acggtcggtc ctaatagttc gagagaggag
     6601 aagtatcgtg agaagcagaa ttcaagccgg tttcaccatg gtggaactga tcgtcgtcat
     6661 cgtcatcctc ggcatcctgg ccgcggtcgc gctgccgaaa ttcatgggcc tcgagcgcga
     6721 ggcgcgtgtc gccgcggtca agagcatggg cggtacgctg ctcagcgccg cgaacatggc
     6781 gcacggtgtc gccatggcgc gcaatgtcac caacggcaac gtgaacatgg aaggctccgc
     6841 ggtcactttc gtcaacggtt atccgagcaa cgccaccgtc tggaacctga tccagagcag
     6901 cgatggcttt acgcgcgccg cgaacacgtt cacgaagacg ggcgccccca ccgccaactg
     6961 ctgggtgcgg tacaaccagg cgaccttggg cgccaacggt gccgtgattg cgccgacgct
     7021 cacgtacaac aacgtggcag cgactcccgc caatggaacc gcgatcgacg ccagcctgcg
     7081 gactgcctgc tgatctgacg gcatcgtctg gagtgtgaag ccgtgcgcat gccccgcaag
     7141 gaatgccgcg gcttcactct gatcgagctc atcgcctcga tcacgatcat cgcgatcctc
     7201 ggagcggtcg catggccgcg gttgagttcc tcgtcgcaac cctacgccga gcgcggttat
     7261 gccgatggcg tcgccgcttc gttgcgtcag gcgcgcgccg tggcgatcgc cagcggatgc
     7321 gacgttcgct tcaccatcac cgcggccggt tataacgcgt tccagcgcgg ggccgccgcc
     7381 aacaatcatt gtgcggtggc cggcgcctgg gtaacgcccg tgcagcgcgg ggatggccag
     7441 tcgctgctcg cgatggcgcc cgccggtgcc gcgctcgccg tgaatcgcca attcgtgttc
     7501 ggcgcggatg gcagcgtcgt gggtggcgcg gtgaacatca ccatcgggcc gcaggtcatc
     7561 gacgtgaatg cgtcgggcgt ggcgtacgga ccatgaaact catgcggaaa caatcgcggg
     7621 gcatgacgct catcgaactg atcgtgtcca tcaccgtgat cgcgatcgcc ggcgcggcct
     7681 tgatgagcac gctcgcgtat ctgaacggca cgggcaatac ctcgatgctc caggcgcagg
     7741 cgcagtccat cgcgaatgcg tacctcaacg aaatcgtggg caagcagttc gtggccgatg
     7801 gggtcgaagc cacacgcgcg cagtacgacg acatcttcga ttacaacaac ctcgacacgc
     7861 ctgccgccag tgatgagcgc ggtaacgcgg ccggcaattt tcgagtgcgc gtggcgatcg
     7921 tgccgggcgc gctcaatgga ctgccggccg ccgacgtgcg gcgcatcgac gtcaccgtcg
     7981 actacggcaa cgcctcgcag gtgatcgcga gcggttatcg cacgaggtat ccatgaacgc
     8041 gcgcggcttc acgctcgtgg agctcgtgat cgcgatcgcg atcgcgtcga tcgtgatggt
     8101 gttctcgatc ctgttcatgc gtgcgccgat cgatgcgtac gaggcgcact cgcgccgcac
     8161 ggatctcgtc aacaatgcgg ccagcgcctg gccgcgcatg gaggcggatc tgcgcaatgc
     8221 gttgcccaac agcgtgcgcg cgcgtcgcaa tggcaatgtg gtggtgctgg agatgctgcc
     8281 ggtgctcggc gtcgcgcgtt acaagacctc gccgtcgacc gtcccgatca ccaccgcggg
     8341 tgtgtatgcc ggcgttacgc ctgcatttct ctcggtcaat acgttgaacg gcaatgcgta
     8401 cgcgctcgcc gggtcgatgg cggccgcgac caacttcacg aaccccgccg gggcggtgac
     8461 gggcgagcag cagataggcg tgaatcccgc gcccgcgttc acttccgatt caccgcgtcg
     8521 gaacctgtat ttcgtcgcgc gtcccgtcac ctggttatgc gatgaagggc tgggcacgtt
     8581 gcagcggtat tccggctaca acatcgccgc gttgcagacc gcgcgcgaca cggccggcga
     8641 gttgacgggt gccggcgccg cggtggagcc ggttgcgcgt ggcctcacga gctgcgtatt
     8701 cgacgtgact ccgcccggca gcacgacgca atcgcagacg gctgccgtgc atctcaccgc
     8761 cacccgtaat ggcgattcgg tcacgctgct gcacagcgtg cgctcggagt acgtgccatg
     8821 agacgcctgc catcgcgcca acgcggcatg tccatggtgg tcgccatatt cctcatcacc
     8881 gtgatcgcga cgctggccgc ttttgcggtg accgtgggta cggcgacgcg cgaggcggag
     8941 aatctgcagt tgcaggcgga ccgagccatg gcggcggcgc gtgccggcat gcagtgggcc
     9001 gcgtatcgcg cgcgggttgc gcaggtgtgt cccgccggcg cgttcaatct caccgagggt
     9061 gcgttgcgcg gctttcgcgt gaccgtgacc tgcgttgcca acaccgggct gcacgcggaa
     9121 ggggctgtga cttaccgggt gttcgatgtg cggtcgttcg cccagtggag caacttcggc
     9181 gccgccaact atgcctcccg caccatcgac gcgcgattca acaccgcccc ttgaaaatgg
     9241 ggcctgaccc catttagtag acgaagggca ccaggcgctt cgtgcgccgc atgtagttga
     9301 tgtagggcga cccgagcgca ttcgcgagcg ccgattcctc ggtggacatg cgccagccga
     9361 acgccagcgc gatgggcacc atgacgatgg ccagcgacgc ccagttggcg agcgtcagcg
     9421 ccaggccgag gaacgcaagc agcgcaccga tgtacgacgg atggcggatg aatcgatacg
     9481 gcccggtatc gatgatctcg tggcgcgagt gaatggccac gttcacggtg aagaaccggc
     9541 ccaggtacgc gatggaatac cagcgcagcg cgagaccgag tccgaacagc gtgcaccccg
     9601 cgaagaacgc gatgcccgca ccgccgcgca tggcggctgc gggcagccac gtcgaagcga
     9661 gaacgcccag cgtcacggcg agcacgatgg tgatccacag gatgcgcagc gtcgcgtcgt
     9721 ccgcatccac gctgtcgtcg cgcgagcgct tcagatacgc gagaccggct tcggaaatcc
     9781 cgtaagccag gcccagcagt gcggggatgg gaaattgcat cgagggccag tctaaaccgg
     9841 cctccggctc cgcagacgtg acggttcacc gtttttttat tgatgcgggg gcgcgttcgc
     9901 gctggcgggc gcgccgtcag tcgagcagcg gagcaatggc gtcgacgacg cgatcgggcg
     9961 aggcgggccg tccgtcgagc cattcggact cgaattccgc gatttcgatg ccaatgacct
    10021 tgcgcttcgc caggatggcc gcgcaggcac gaaggtcttc gagcgagagg ccgccggtga
    10081 cctggtattc gatgggcacg atgccgggtt cgagtacgtc gcagtcgatg tggacgtaga
    10141 cgtcacggtc gcccaccgcc gcggcgagcc gcgcggcgag gttggggccg ggcgccacca
    10201 gcttgagttc accgctgccg atgagctcct tttcatgggg atcgaggtct cgcgcaccca
    10261 ccagcaccac gttcgacagc ttgagcccgg cgcccaggcc gctatcccaa tggcccgcgg
    10321 cacccgtgag caccatgccg cccaggtagg gcacggcatt ggagctgggc agattcgaat
    10381 cgccgtgcgc gtcgaaccac accacacagg cgtccggatg cgcttcggcg atcaccggga
    10441 cggtggcgag gcccgcggcg cagcggccga tggctgccac cgcgcgatgt ccgtcgcgaa
    10501 tggcggcgcg caacgcgtcg gcgagcgcac tgagctcacc gcgggccgcc gcgagttgct
    10561 cgtcccacgc ggtggtcggc agcggtgcgt gcggcgtgct cacataggtg acgtcgaggc
    10621 cgtaacggcg cgccagcagt gcgcccagca tctgcgcgcc cgcggtgccg cgggaattgc
    10681 gatcgccggc gcggccgcgg tacacgatga gcgtggatga tttcatcggc cgcgagacta
    10741 accactttac gcccgttccg ctacttgagc ggccgccacg gtcgaggccg ctgccgacca
    10801 ccgtgctact cacacgtggc gcaccggccc gagagtactc catgccccgt gggaagcggt
    10861 tcgtgcgggt cgtgctcgtg gatcgagagc aggcgccgct cgccgccgcg cctaacaaag
    10921 accatggcgc cgcttgcacc ttcgctctgc cagccttcgc gctcgagcgc ggcgagaccg
    10981 tcggcgaagg atccgcgcag gtcgctgccg gcgggtagtt cggtggcgtc gagcacgttg
    11041 tggaacatgt cgcggagcac gagccagcgc gagagccggg acgtggccat ggggtcgtat
    11101 ttgcttcgtt tggttgtcat gccgcgaacg ttatcgaggt gtgttcgcag ccgtccattc
    11161 agtagtgcgc gccgcagcga gctgtatgga gcgcgttgaa tgacgaggag acgacgataa
    11221 gtgcgcgact cgtttgttct ttttatgggc ggcgccgaag ccggcgcttg cgaccgggcg
    11281 gcggcccccc ataaaaaaag gccgcgttaa aacgcggcct ttttgcggcg tgcagtgtcg
    11341 cattcataag gtgcttgctg ccgccactcg tggattcacg tcggctaatc gggaggattg
    11401 cttcatgaat ctacggtttc agtatccacc ctgccgcaag ccgcgcaaat gggcgtgctg
    11461 ctgaatcttc ggtagacaat ttcctacagc gtgcggacta ggcgcgtccc cgtttgctgc
    11521 aaatcaggaa tgtccccatt tcaggcgcct atgaaaggcg gatcaggccc cgtttgtggg
    11581 cgacggccac ggcctcggtg cggctgatgg cgtctaactt cccgaggatc gccttgacgt
    11641 gtgtcttcgc ggtgccctcg gtgatcgcga gccggcgcgc gatgtccttg ttgctgcggc
    11701 cttcggccac gagctggaga acgtcgagtt cgcgcgtggt gagactgggc ttcgacatgc
    11761 gctgcagcgc tttcgcggcg atgcccgaag aggtataggg gcggtcgtcg gccacggcgc
    11821 ggatcgcgga aagaatttcc tggcgcgggg catccttcag caggtatccc ttcgcgccct
    11881 ggctcaggct gcggaagatg tcttcgtcgc cgtcgtacgt ggtcatgatg agcaggcagg
    11941 ctttggggta tttctcgagc accttctgca cgacggcggc gccgtccatc ttcggcatgc
    12001 gcaggtcgag caccatcacg tcgggcttgt gcagctcata caggtccagc gcttcggcgc
    12061 cgtcgccggc ctcggccacc acctgcatgt cgggctgctg attgacgatg gccgcgagtc
    12121 cgtcgcgcac cacggggtgg tcgtcggcga ggatcacgct gatcttgcgt gccggcacct
    12181 tggcggcgat tttcatcgcg ccaccgggcg cggcaggttg acgatgatgc gcgtgccttc
    12241 accgggtttg ctgtcgatgt ggaacacgcc gccaatggcg ccggcgcgtt cgcgcatgtt
    12301 gttcaggccg taaccttgct gggcataaag ctcggggctc agcgtcatgc cgcggccatc
    12361 gtcgatcacg ctcatggtcc accgcgcctg ctcttcgagc aacacgatgc ggacggtggt
    12421 cggat
//
LOCUS       DATDEU010000407         4322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1099705, whole genome shotgun sequence.
ACCESSION   DATDEU010000407 DATDEU010000000
VERSION     DATDEU010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099705"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="VIV63_09735"
     CDS             complement(<1..1951)
                     /locus_tag="VIV63_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124923.1"
                     /translation="MNGLARSGAAPLLRSTGLLIRLRLRRLTNQWTALSQGKKKPGDK
                     TRTGNPGKKSSKVIMYLAWPLMLFVFGSISFTAVLNLHGALDPPDTFWVTVEFSTALS
                     IGIAYLLVVMWTASLLVTVASGELAKPEWDLEWLITLPVRSDTLLWARIAERSVVNPS
                     GVLALLPACTAIAWFSGYRWMAPIIGTLSAWPLLLLAALVRTLIDTGLRLRLRAAQLR
                     NLHAVLSVLSIVLMYLAFSFGLKGKSEFMLNLAASMPDWLRYTPMGLTVSVINERNFS
                     DTLQLLLALFAEVAVITWLGVRLLRHVLRNGVVAGGAREGARNTPDESAAAAEAPAPS
                     RFRMITMSPVQRRELTLLRRDRNFLVQSLVLPLLIVGGQMLLGTSSAASTMWTNPNVL
                     ASVAFGLAAYSLSMSAFQTLNSEGQALWLLYTFPCPIEDVLKDKAKLWGVLTLAYPLG
                     MFAVGAVVTPSLDWKFLGAMLTALLGIPIYAFIAVALGVFGSNPLEQQQNQKVKPAYV
                     YLYMTLAALYVYAIVTPQPAQRLIFMVLSMLLAAALWQKARDQLGFLLDPDASPPPRV
                     STSDGLIAAMLFFVAQAVAAAVIIGRGRVTGAAVLFAFAIGGAITYALMRWVYARAKT
                     EGVPRMFGPDANRPLLGLVAGLAAGA"
     gene            complement(1948..2691)
                     /locus_tag="VIV63_09740"
     CDS             complement(1948..2691)
                     /locus_tag="VIV63_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6124924.1"
                     /translation="MIEFQQLSRTYGDFTAVRPLSLEVRSGEVFGFLGPNGAGKTTTI
                     RMMMGILVPSGGRALINQLDCHTQRAEVQRHVGYLPDNPIYYDFLRGREILQFVAEMH
                     GMGRREAAQQAVRMLAEFGLEEVIDEFAVNYSMGMKKKLGLACALIHDPQVLILDEPI
                     NGLDPRAAREVQDRLRQAAASGKTIFMSTHLLDMAEKVCTRVGIIHRGELTATGTLEE
                     LRARVHQNASLEEVFLKVTDEEPARVDAP"
     gene            2728..4185
                     /locus_tag="VIV63_09745"
     CDS             2728..4185
                     /locus_tag="VIV63_09745"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY6124925.1"
                     /translation="MKNNNEMSPWVKADLPEPPVPRGLSWIGVCGPGVIVLGMSIGSG
                     EFLLGPATFVKHGLSLLWVTLVAVFFQTVFNTELMRYTVATGEPVVTGFMRTRPGKTF
                     WAWFYAGLYFLQIGWPAWAATAAGAIFFLFADRLAKPPADSATIYMIGIVTFLACVAV
                     LAFGKRIARTLEILNWVLVACILGSFVVLGLIYVPASTWLAGLAGFAGFDAVHGVFSF
                     LPDNLDILLLGALVGYSGGGGVLNLTLSNWARDKGYGMGIRVGHIAGAVGGVKVKVAQ
                     TGFIFNPDAPNLARWRGWWRIVRVDQWGIFFVGALLGMMLPALLYVTFVAPGTDIKGL
                     AISAVLAQAIGATGGAWLGMLVALLGVWILFKTQLDCVEGMTRSITDILWAGSDRVRA
                     WRGGDIRAVYYAVLTAVVIWGIIALKLAAPVMLLQIGANIAGAVFVIASLHVLYLNTR
                     VLPAALRPPMWRRVALVGMCLFYGFFVTLVTRSFL"
BASE COUNT          715 a         1359 c         1472 g          776 t
ORIGIN      
        1 aggcgccggc cgccaggccc gcgacgagtc cgagcagggg acggttggcg tccgggccga
       61 acatgcgtgg cacgccttcg gtcttcgccc gtgcgtagac ccagcgcatc agcgcgtagg
      121 tgatggcgcc gccgatggcg aacgcgaaca acaccgcggc gcccgtcacg cggccgcggc
      181 cgatgatgac ggcggcggcc acggcctgcg ccacgaagaa cagcatggcg gcgatcaacc
      241 cgtccgacgt ggagacgcgt ggcggcggcg acgcgtccgg gtcgagcagg aagccgagct
      301 ggtcgcgcgc cttctgccac aacgcggcgg ccagcagcat cgacaacacc atgaagatga
      361 gccgctgcgc aggctgcggc gtgacgatgg cgtagacgta gagcgcagcc agcgtcatgt
      421 acagatagac gtaggccggt tttactttct ggttctgttg ctgctcgagc ggattgctgc
      481 cgaacacccc gagcgccacc gcgatgaagg cgtagatggg aatacccagc agcgccgtga
      541 gcatcgcgcc caggaatttc cagtcgagcg atggcgtcac caccgcgccc accgcgaaca
      601 tgccgagtgg ataggcgagc gtgagcacac cccagagttt ggccttgtct ttcagcacgt
      661 cctcgatggg gcacgggaac gtgtacagca gccacaatgc ctggccctcg ctgttcagcg
      721 tctggaacgc cgacatgctc agcgaatacg cggcgagacc gaatgctacc gaagcgagca
      781 cgttgggatt ggtccacatg gtgctggccg cgctcgaggt gccgagcaac atctgtccgc
      841 cgacgatcag cagcggcagc accaggctct gcacgaggaa attccggtcg cggcggagca
      901 gcgtcagctc gcgccgttgc accgggctca tcgtgatcat gcggaatcga ctgggcgcgg
      961 gcgcttcggc ggcggctgcg ctctcgtccg gtgtatttcg cgcgccctcg cgggcgccgc
     1021 cggcgacgac cccgttgcga agcacgtgcc gcaggaggcg cacgccgagc caggtgatca
     1081 cggccacttc ggcgaacagc gcgagcaaca gctgcaaggt gtcggagaag ttgcgctcgt
     1141 tgatgacgct cacggtgaga cccatgggcg tgtagcgcag ccagtcgggc atcgacgcgg
     1201 cgagattcag catgaattcc gacttgccct tcaggccgaa ggaaaaggcg agatacatga
     1261 gcacgatcga gagcaccgac aggaccgcgt gcaggttgcg caattgcgcc gcgcgcaggc
     1321 gaagacgcaa gccggtgtcg atgagtgtgc gcaccagcgc cgcgagcagc agcagcggcc
     1381 atgcggacaa cgttccgatg atcggcgcca tccagcgata tccggagaac caggcaatgg
     1441 ccgtgcacgc cggcagcagc gccagtacgc ccgacggatt caccacgctg cgctcagcga
     1501 tacgcgccca cagcagcgtg tcggagcgaa ccggcagcgt gatcagccac tccagatccc
     1561 attccggttt ggcgagttca cccgatgcca cggtcacgag cagcgaggcg gtccacatca
     1621 ccacgagcag gtaggcgatg ccgatgctca gcgcggtgct gaattcgacc gtcacccaga
     1681 atgtgtcggg cggatcgagc gcgccatgca ggttgagcac ggccgtgaag gagatcgatc
     1741 cgaagacgaa caacatgagc ggccaggcca ggtacatgat gaccttgctg ctcttcttgc
     1801 cggggttgcc ggtgcgcgtc ttgtcgccgg gcttcttctt gccctgcgaa agcgcggtcc
     1861 actgattggt gagccggcgc agccgcaggc ggatcagcaa cccggtgctc cgaagcagcg
     1921 gcgcagcgcc acttctggca agtccgttca cggagcgtcg acccgggcgg gctcttcgtc
     1981 ggtgaccttg aggaacactt cctcgagcga ggcgttctga tgcactcgcg cgcgcagttc
     2041 ctcgagcgtg ccggtggccg tgagctcgcc gcgatggatg atgccgacac gcgtgcagac
     2101 cttttccgcc atgtccagca gatgcgtgga catgaagatc gtcttgccgg atgccgcggc
     2161 ctgccgcagc cggtcctgga cttcacgcgc ggcgcgcggg tcgagtccgt tgatgggctc
     2221 atcgaggatc agcacctgcg gatcgtggat cagcgcgcag gccaggccga gcttcttctt
     2281 catgcccatg gaatagttga ccgcgaattc gtcgatcact tcttcgagcc cgaactcggc
     2341 gagcatgcgg accgcctgtt gcgcggcctc gcggcgtccc atgccatgca tctccgcgac
     2401 gaactgcagg atctcgcggc cgcgcaggaa atcgtagtaa atggggttgt cgggtagata
     2461 gccgacgtgg cgctgcacct cggcgcgctg ggtgtggcag tccaattggt ttatcaacgc
     2521 gcggccgccg ctcggaacca ggatgcccat catcatgcgg atggtggtgg tcttgcccgc
     2581 gccgttcgga ccgaggaagc cgaagacttc gccgctgcgc acctcgagcg agagcggccg
     2641 tacggccgtg aagtccccgt aggtcctcga gagctgctga aattcgatca tgcgccgcag
     2701 tgtatgctca ggcgcaggag ggggtcgatg aagaacaaca acgagatgtc accctgggtg
     2761 aaggcggatc tgcccgagcc accggtgccg cgcggactgt cctggatagg cgtgtgcggc
     2821 ccgggtgtca tcgtgctcgg catgtcgatc ggcagtggcg agttcctgtt aggccccgcg
     2881 actttcgtca aacacggcct gtcgctgctt tgggtgacgc tggtcgccgt attcttccag
     2941 accgtcttca acaccgagct catgcggtac accgtcgcca ccggcgaacc ggtggtcacg
     3001 ggcttcatgc gcacacgtcc cggcaagacc ttctgggcct ggttctacgc cgggttgtat
     3061 ttcctccaga taggctggcc cgcgtgggcg gccaccgcgg cgggcgccat cttcttcctc
     3121 tttgccgacc ggctcgcgaa accgcccgcg gattcggcca ctatctacat gatagggatc
     3181 gtcacgtttc tcgcgtgcgt cgccgttctc gcgttcggga aacgtatcgc ccgcacgctc
     3241 gagatactca actgggtact ggtggcgtgc attctcggca gcttcgtggt gctgggcctc
     3301 atctatgtac ccgcgagcac gtggttggcg ggcctggccg gcttcgcggg attcgatgcc
     3361 gtgcatggcg tgttcagctt cctgccggac aacctggaca tcctgctgct gggtgcgctg
     3421 gtgggttatt ccggtggcgg cggcgtactc aacctgacgc tgtcgaactg ggcgcgcgac
     3481 aagggctacg gcatgggcat ccgcgtggga cacatcgcgg gcgcggtggg cggcgtgaag
     3541 gtgaaggttg cgcagacggg tttcatcttc aatcccgatg cgcccaatct cgcgcgctgg
     3601 cgcggctggt ggcgcatcgt ccgcgtcgat caatggggca tcttcttcgt cggcgcgttg
     3661 ctgggaatga tgctgccggc gttgctgtac gtgacgttcg tcgcgccggg caccgacatc
     3721 aaggggctcg ccatcagcgc ggtgctggca caagccattg gtgcgaccgg aggcgcgtgg
     3781 ctgggcatgc tcgtggcgct gctcggcgtg tggatcctct tcaagacgca gctcgactgc
     3841 gtggaaggga tgacgcgttc gatcaccgac atcctgtggg ctggcagcga ccgcgttcgc
     3901 gcctggcgcg gtggcgacat tcgcgcggtg tattacgccg tgctgaccgc ggtggtgatc
     3961 tggggaatca tcgcgctcaa gctggcggcg cccgtgatgc tgctgcagat tggcgccaac
     4021 atcgcgggcg ccgtcttcgt catcgcgtcg ctgcacgtgt tgtacctgaa cacgcgcgtg
     4081 ttgccggccg cgctgcggcc gccgatgtgg cgccgcgtgg cgctggtcgg catgtgcttg
     4141 ttctatgggt tcttcgtgac gctggtgacc cggtcgtttc tctgacgctg cgattcagcg
     4201 cttgcggctg atctcgatgc cgttgcccac ctcgagcagc accgaatcca tgtctcccga
     4261 gtcgcgcacg cgcttgcggt aggcgtcggc atgcgggcgc gcgctttcgg gcaacagcat
     4321 gt
//
LOCUS       DATDEU010000408         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1099833, whole genome shotgun sequence.
ACCESSION   DATDEU010000408 DATDEU010000000
VERSION     DATDEU010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099833"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VIV63_09750"
     CDS             complement(<1..512)
                     /locus_tag="VIV63_09750"
                     /inference="COORDINATES: protein motif:HMM:NF021158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HEY6124926.1"
                     /translation="MKLRLGLVCLAAMLGACAEKEEVPTVAEITGTATYRERIMPPPD
                     ATLDVVLEDVTRADAPAEEIVRTMQRLSTAPPYSISIWFDPARIIPNHRYNVRAKITA
                     GGKLWFISDTAHPVLGPKDVRKVDIVMRAITEADTTPTPPSVQFRGMYSYMADAGWFV
                     ECLSGSRMAVA"
     gene            complement(545..1162)
                     /locus_tag="VIV63_09755"
     CDS             complement(545..1162)
                     /locus_tag="VIV63_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124927.1"
                     /translation="MKALLCTLSLLGASAAHAQQMDMDAMMKWGSADLIRYHVVGVYQ
                     GKPSIASDGSGRADVTDRVVIDFSWKLSEMKLVGAPTFQNTKTTLSNPTDREPSCLPP
                     VLKGEYEHYELLGIKEGLSGSLEFTVQTTHPIVEVAQFCTASRKSVPAKKNVRAEDFV
                     VVSPVTFGMPLPDSDDLRISKDKKSLIAKKNGWTWTYTPAIAPAR"
     gene            complement(1159..>2714)
                     /locus_tag="VIV63_09760"
     CDS             complement(1159..>2714)
                     /locus_tag="VIV63_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124928.1"
                     /translation="VESASAVLRVTTPGPVSILESPPNVTIRAGETANICMAFGGTPP
                     FAVQMSRWQNIEWTPIGPRHIFPDNGRHCIATPVLQSADDGAEFLFLGANDEGGIVEA
                     MTRIVTVTVTSQAVVTNTTLASRSTSGVTANNRSGLPSLSADGSIVAFISDGTNLVPG
                     FVGSAFTAGNAYVRDLSTGVTTLVNVTPAGTQSQSPYGVIGLKLAAGGRHAIFSSLAG
                     DLVADDTNGSQDVFVRDLQTGVTTRVSLHADGSQITGAGNSQFDMQVNISEDGRFVSF
                     VSNQDLIGSDGPGGYSLYLYSRQTGFMQRVTSGPSPVGYSTMSANGEHLAYRDATFAP
                     DRNFIVHFDAEAGVLEEVFSIDSSNNVSYLAQGVSISDDGRYIAFALRSPTTFNGSLF
                     TQIMAIDRNNPGNITVASGDSNGFGNGHSIHPEVSDDGHVLFMTNAGSLTSDFANSQI
                     TALVVRDLHTTDLSVASRRADGSSIAILGGYAYHAISRDGSVVAFAADEFDMSGGARE
                     YQVYVAPRP"
BASE COUNT          479 a          812 c          907 g          516 t
ORIGIN      
        1 gccaccgcca tgcgacttcc cgaaaggcat tcgacgaacc agccggcgtc ggccatgtag
       61 ctatacatgc cgcggaattg cacggacggc ggcgtgggtg tggtgtcggc ctccgtgatc
      121 gcacgcatca cgatgtccac cttgcgcacg tccttcggcc ccaacacggg atgtgcggtg
      181 tcggagatga accacagctt gccgcccgcg gtgatcttgg cgcgcacgtt gtatcgatgg
      241 ttcgggatga ttcgcgcggg gtcgaaccag atggagatgg aatagggagg cgcggtgctg
      301 agccgctgca tggtccgcac gatttcctcg gccggcgcat cggcgcgcgt gacgtcctcg
      361 agcacgacat cgagcgtggc atccggcggc ggcatgatgc gttcgcggta ggtagcggtg
      421 ccggtgattt cggcaaccgt cggcacttct tccttttccg cgcaggcgcc gagcatggcc
      481 gcaaggcaca ccaacccgag tcgtaatttc atggcgggag cctacacccg ccttgtggac
      541 gcgactatcg agcgggcgca atggccggcg tgtacgtcca ggtccagccg ttcttcttcg
      601 cgatcagcga cttcttgtct ttggaaatgc gcagatcgtc ggagtcgggc agcggcatgc
      661 cgaaagtcac cggcgacacc acgacgaaat cctcggcccg cacgttcttt ttcgccggaa
      721 cggatttgcg gctggcggtg cagaactggg ccacctcgac gatggggtgg gtggtctgca
      781 cggtgaactc gagcgagccg ctgagccctt ccttgatgcc gagcagttca tagtgttcgt
      841 actcgccctt gagcaccggc ggcaggcacg agggctcgcg gtcggtggga ttgctgagcg
      901 tggtcttggt gttctgaaac gtgggcgcgc ccacgagttt catctcggac agcttccagc
      961 tgaagtcgat gacgacgcgg tcggtgacgt ccgcgcggcc ggagccatcg ctcgcgatgg
     1021 acggcttgcc ttgatagacg ccgacgacgt gatagcgaat gagatccgcg gatccccact
     1081 tcatcatggc gtccatgtcc atctgctgcg cgtgggcagc ggaggcgcct agcaaactca
     1141 acgtgcagag gagcgccttc atggccgcgg cgccacgtaa acctggtact cacgcgcgcc
     1201 gcccgacatg tcgaattcgt cggcggcgaa cgccacgacg gagccatcgc gcgatatcgc
     1261 gtggtaggcg tatccgccca ggatcgcaat gctgctgccg tcggcccggc gcgacgccac
     1321 gctcaagtcc gtggtgtgca ggtcacgcac gacgagcgcg gtgatctgac tattcgcgaa
     1381 atcgctggtg agactgccgg cattggtcat gaagagcaca tgcccgtcat ccgacacctc
     1441 tggatggatg ctgtgaccat tgccgaatcc attggagtcg ccactcgcga cggtgatgtt
     1501 gcctggattg ttccggtcga tcgccatgat ttgagtgaag agtgatccgt tgaacgtcgt
     1561 gggagatctc aaggcaaagg cgatgtatcg accatcgtcc gagatgctga cgccctgcgc
     1621 gagatacgac acgttgttgc tgctgtcgat cgagaaaacc tcctccaaga cgccggcttc
     1681 ggcatcgaag tggacgatga aattgcggtc cggcgcgaaa gtggcatcgc gatacgcgag
     1741 gtgctcgcca ttggccgaca tcgtcgagta accgacgggt gaaggtccgc tggtgacgcg
     1801 ctgcatgaaa ccggtttgcc tgctgtacag gtacagcgag tagccaccgg gtccatcgga
     1861 tccgatgagg tcctggttcg atacgaaact cacgaaccgg ccgtcttccg agatgttgac
     1921 ctgcatgtcg aattggctgt tgcccgcacc ggtgatctgc gagccatcgg catgcagact
     1981 gacacgcgtg gtgacgccgg tctgcagatc gcgcacgaac acgtcctgcg agccgttggt
     2041 gtcgtcggcg acgagatctc ccgcgagcga gctgaaaatc gcgtgacgcc cgcccgcggc
     2101 gagtttgagt ccgatgacgc cgtagggcga ctgggattgc gtgccggccg gcgtcacgtt
     2161 gacgagcgtg gtgacgccgg tggagaggtc ccgcacgtat gcattgcccg ccgtgaaggc
     2221 cgagccaaca aaccccggca cgaggttggt accgtcgctg atgaaagcga cgatgctgcc
     2281 atcggccgat aggctgggca ggccgctgcg gttgttagcc gtgacgccgc tcgtcgaacg
     2341 gctggcgagc gtcgtgttgg tgacgacggc ttgtgaggtc accgtgacgg tcacgatgcg
     2401 cgtcatcgcc tcgacgatgc cgccctcgtc attggcgccg aggaacagga actccgcgcc
     2461 gtcatccgcg gactggagta cgggcgtcgc gatgcaatgg cggccgttgt ccgggaagat
     2521 gtggcgtgga cctatcggcg tccattcgat gttctgccag cggctcatct gcacggcgaa
     2581 cggcggtgtg ccgccaaaag ccatgcagat gttcgccgtt tcaccggccc ggatggtcac
     2641 gttgggtggc gattccagga tgctcacggg cccgggcgtc gtcacccgca gcacagcgga
     2701 cgcactttcg acga
//
LOCUS       DATDEU010000409         6843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_222666, whole genome shotgun sequence.
ACCESSION   DATDEU010000409 DATDEU010000000
VERSION     DATDEU010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222666"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /locus_tag="VIV63_09765"
     CDS             <1..2097
                     /locus_tag="VIV63_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6124929.1"
                     /translation="GGGLVLGIRFANRREGASAATGSGRILAPNAFVRVAPDDTITVV
                     IGKSEMGQGIYTGLAMALAEELDVDPARVNVEMAGADPAFNVPFMPIQFTGGSMSTST
                     TYMQLREAGARARAMLLAAAAQRWGVDVAELATSDGKVTRGSKTLTYGALADAASQLP
                     VPEKVALKDPANFLYLGKPQKRLDAPMKVDGSAKFGIDMRVPGMLFAVIARPPVVGAT
                     FEKMNDTAARAVAGVVDVKVIPAGVAVYGTNTWAAKRGRDALEISWNEGPQANYSTVA
                     QRREYQRLLGRPGAVARNTGDVKAALSTAARRMDVEYELPYLAHSPMEPLNCLADVRA
                     DGCDLWLGTQMQSPDRDAAARELGLDPSKVVLHNTFLGGGFGRRAQRNSDVVLEAVQL
                     SKAVGKPVLSVYTREDDVRGLSYRPHVMSRVRGGVDAAGMPVAWQQTIVSQGVLRGGW
                     TEAFIPKGQAFDQSSVEGASDMPYDIPNVLVDTHEGTSKVPVLWWRSVGHSHTGFTVN
                     CALDELAVLGGQDPVEMRRKLLGKHPRQLAVLNAVAEASNWAGGAPAGRGRGVALHES
                     FGSIVAEVAEVSVEDGNVRVHKVWCAIDCGFAANPSGVIMQMESGINYGLTAALYGEI
                     TFAGGKVEQSNFHDYQILRLNEAPEIFVTIVNSGEAMGGAGEPGTPPIAPAVANAIYA
                     ATGKRVRKLPIAKNLV"
     gene            complement(2117..3313)
                     /locus_tag="VIV63_09770"
     CDS             complement(2117..3313)
                     /locus_tag="VIV63_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY6124930.1"
                     /translation="MDGKIITWRRDFHQNPELSNREVRTSGIVAEHLKKLGLEVDTGI
                     ARTGVVALLKTGKPGPTIALRADMDGLPVVERTDVPFKSLAKSSYRGEEVGVMHACGH
                     DAHTAILMGVAEALTKARGSLRGNILFVFQPAEEGAPPGEVGGASEMLKAGIFDKYKP
                     EVAFGLHMWASLNTGIIGYRGGPFMAGSQAWKLVVNGKQTHGSRPWQGVDPIVVTAQI
                     INAMQTVVSRQVDITQNPAVLSAGIIKGGIRNNIIPDSVEVIGTIRTFDAKQKQQIYD
                     AMKRLVENTAEASGATATFEFDTYANPVTYNNPELTARVLPSLQKVAGADQVKEIPLI
                     TGSEDFAYFGEKVPSFFFMVGITPKGTNVLTAPANHSPLFYMDEAAIPLATRAITAVA
                     VDYLSK"
     gene            3490..4647
                     /locus_tag="VIV63_09775"
     CDS             3490..4647
                     /locus_tag="VIV63_09775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6124931.1"
                     /translation="MHVVDATLFYSPTSGGVKRYLLAKHEWMREHTGWRHSLLVPGES
                     RMHAPGDISTIAGGLVPGAFNYRLPLNPVRWSRAIDALEPDLIEVGDAFHPAWAAANV
                     ANRRGIPLIAFYHSNFPQLAGRRLGKSVQRFIEWYVKLTYERCEQVLAPSRYMCDYLH
                     SIGVTHATCQPLGVDVDTFNPDKRGRDLAAELNLPRGTRVLVFAGRFSAEKNIPVLHE
                     TFRRLGDDYHLLMIGGGESGREGNVTRIPYCRDNSTLASYFASADAFVHAGVHETFGL
                     VVVEAMACGRPVVAMRAGALPELVDASAGNLAEPHEDPMVAAANLADAIVHAYERDVD
                     VLGAAARRHVLANYSWSRTLQTLMTRYQSAVSARRLPSMANGLAGAETTTH"
     gene            complement(4648..5226)
                     /locus_tag="VIV63_09780"
     CDS             complement(4648..5226)
                     /locus_tag="VIV63_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124932.1"
                     /translation="MVELRDLTPDERREVQQRFEMLASKRFAPQAKRPRHEVWWPKAP
                     LDGRAFPEGIQSPDGEDSFNESVRLLGFLNGVCGGEFYIANLSAPKPFFRHTGKLDAE
                     LAGWIRSRLVSNPTCLLASTLDVFLIVDSQMNYSVIGGPPEVIEKFEKRFGGAEALQR
                     TFASYVDQFRVGSGLEDVKWAQDYLQKWSGWL"
     gene            complement(5226..6266)
                     /locus_tag="VIV63_09785"
     CDS             complement(5226..6266)
                     /locus_tag="VIV63_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124933.1"
                     /translation="MASKKSLSFALEPRANTSIDDYVADIAWAPDSKSFAIAGGEGKV
                     ALARTEADSLTVEVLGEHLLGALSIVWRPNANVFATSGQDGSVALWDGTSGKEIKRWK
                     PAPTPTQALSFSPDGEILASAAGKVVTLWSPLGEKIHAFAPAASTAVALSFDRPGTDL
                     GVALNGEIAVHRIEKARYETRRYKWLAACLTVNFSGNGRFLASGMADGTLHFWNRSTG
                     KDSQMRGYDGKLELVAWSDNSRYLASSAGNEVVLWDFSGKGPEGTRPIVLSGHTERVD
                     SFSWQPGSEHFVSAGRDWRLTLWRPAKAKQPIDVQMLDSEISVVRWSPDGKRVVAGEK
                     NGRVTLFELVAR"
     gene            complement(6269..>6843)
                     /locus_tag="VIV63_09790"
     CDS             complement(6269..>6843)
                     /locus_tag="VIV63_09790"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6124934.1"
                     /translation="TAATPAALEAAIEPAVRAFAEAATVVPCHGTLEPAPSHNAIDIS
                     HPGADFKLTIAKAGAYALFCEHHPLEFGLHLAGQRPAAERKFASHHHEDDISSIGISD
                     PRALDPVKVNQWFDYLLKTRGQDIFRMKGVLNVKGEDRRQVFHGVHMMFDAQAERPWG
                     ATPRVNSLVFIGRNLDRAELEAGFESAVAK"
BASE COUNT         1168 a         2185 c         2239 g         1251 t
ORIGIN      
        1 ggcggtggac tggttctcgg tattcgtttt gcgaaccggc gcgaaggagc gtcggccgcc
       61 acgggttccg gacgaattct ggctccgaat gcgttcgtcc gggtcgcccc ggacgatacg
      121 atcacggtgg tgatcggcaa atccgagatg ggccagggca tctacaccgg tttggccatg
      181 gcgctggccg aagagctcga cgtggacccg gcacgcgtga acgtggagat ggcgggcgcg
      241 gatcctgcgt tcaacgtgcc gttcatgccc atccagttca ccggtggcag catgagcacc
      301 agcaccacgt acatgcagct gcgtgaggcg ggcgcccgtg cgcgcgccat gttgttagcc
      361 gcggccgcgc agcgctgggg cgtcgacgtg gccgaactcg ccacatccga cggcaaggtc
      421 acgcggggtt cgaagaccct gacctatggc gcgctggcag acgccgcctc gcagctcccc
      481 gtgcccgaga aagtggcgct caaggatccc gcgaattttc tctacctcgg caaaccgcag
      541 aagcgcctcg acgcgcccat gaaggtggat ggcagcgcga agttcggcat cgacatgcgt
      601 gtgcccggca tgttgtttgc cgtcattgcg cggccaccgg tggtcggcgc gacgttcgag
      661 aagatgaacg acaccgccgc gcgcgcggtg gcgggcgtcg tcgacgtcaa ggtgatcccc
      721 gcgggggtgg cggtctacgg cacgaatacc tgggcggcca agcgcggccg cgatgcactc
      781 gagatttcct ggaacgaagg gccgcaggcg aactactcga ccgtggcgca gcgccgtgag
      841 tatcagcggt tgctcggcag acccggtgct gtcgcgcgca ataccggcga cgtcaaggcc
      901 gcgctgagca cggccgccag gcgcatggac gtggagtacg agctgccgta tctcgcgcat
      961 tcgcccatgg agccgctcaa ttgcctggcc gatgtgcgtg cggatggatg cgatctgtgg
     1021 ctcggcacgc agatgcagtc ccccgatcgc gatgccgcgg cacgcgagtt gggcctcgat
     1081 ccgtcgaagg tcgtcctgca caacacattc ctgggtggcg gattcggccg gcgcgcacag
     1141 cgcaattcgg acgtcgtgct cgaggccgtg cagttgtcga aggcggtcgg caagccggta
     1201 ctcagcgtgt acacgcgcga agacgacgtg cgcggtcttt cctatcgccc gcatgtaatg
     1261 tcacgcgtgc gcggcggcgt ggacgccgcg ggcatgccgg tggcctggca gcagaccatc
     1321 gtgagccagg gcgtgctgcg cggtggctgg accgaggcgt tcatccccaa gggccaggcg
     1381 ttcgatcagt cgagcgtcga aggcgcgtcc gacatgccct acgacattcc gaacgtgctg
     1441 gtcgacacgc atgaaggcac cagcaaggtg ccggtgttgt ggtggcgttc ggtggggcat
     1501 tcgcacaccg gcttcacggt gaattgcgcg ctcgatgagc tcgcggtgct cggcgggcag
     1561 gatccggtcg agatgcgccg caagttgtta ggcaagcatc cgcgacagct cgcggtgctg
     1621 aacgcggtgg ccgaggccag caactgggcg ggtggcgcgc cggcgggccg ggggcgcggc
     1681 gtcgccctgc atgagtcctt cggcagcatc gtcgccgagg tcgcggaggt gtccgtggag
     1741 gacggcaacg tgcgcgtgca caaggtgtgg tgcgccatcg actgcggttt cgccgcgaat
     1801 ccgagcggcg tcatcatgca gatggagagc gggatcaact acggtctgac ggcggcgctg
     1861 tatggcgaga tcacgtttgc cggcggcaag gtggagcaga gcaacttcca cgactaccag
     1921 atcctgcggt tgaacgaggc gccggagatc tttgtgacca tcgtgaattc gggtgaagcg
     1981 atgggcggcg cgggggagcc cggtacgccg ccgatagcgc ccgcggtggc gaacgcgatt
     2041 tacgcggcga cgggcaagcg cgtgcgcaag ctgccgatcg cgaaaaacct ggtttgaggt
     2101 ggtgcccgga accgccctac tttgacaagt agtccaccgc gacggcggtg atcgcccgcg
     2161 tcgcgagcgg aatcgccgcc tcgtccatgt agaagagcgg cgagtggtta gccggcgcgg
     2221 tcagcacatt ggtgcccttc ggcgtgatac ccaccatgaa gaagaagctc ggcaccttct
     2281 cgccgaaata ggcgaaatcc tctgaaccgg tgatcagcgg aatctccttc acctgatccg
     2341 cgcccgcgac tttctgcagg ctcggcaaca ctcgcgccgt gagttcgggg ttgttgtagg
     2401 tgaccgggtt cgcgtacgtg tcgaactcga acgtggccgt ggcgccgctc gcttcggcgg
     2461 tgttctccac gagccgcttc atggcgtcgt agatctgctg cttctgcttc gcgtcgaacg
     2521 tgcggatggt gccgattacc tcgaccgaat ccggaatgat gttgttgcgg ataccgccct
     2581 tgatgatgcc ggcggacagc accgcgggat tctgcgtgat gtcgacctgg cggctcacca
     2641 ccgtctgcat ggcgttgatg atctgcgccg tgacgacgat gggatcgacc ccctgccacg
     2701 gccgcgagcc gtgggtttgt ttgccgttca cgaccagttt ccaggcctgc gatccggcca
     2761 tgaacggccc gccgcgatag ccgatgatgc cggtgttcag cgaggcccac atgtgcaggc
     2821 caaacgcgac ttcgggtttg tatttgtcga agatgccggc cttcagcatc tctgacgcac
     2881 cgcccacctc gccgggaggc gcgccttctt cggcgggctg gaacacgaac aggatgttgc
     2941 cgcgcaacga gcccctggcc ttggtgaggg cctcggcgac acccatgaga atggcagtgt
     3001 gagcatcgtg cccgcaggca tgcatgactc ccacttcttc gccgcggtag ctagacttcg
     3061 ccagcgattt gaaaggaaca tcggtacgct cgacgactgg cagaccgtcc atgtcggcgc
     3121 ggagcgcgat ggtgggtccg ggcttcccgg tcttcagcaa tgcgaccacg ccggtcctgg
     3181 cgatgcccgt atcgacttcg aggccgagct tcttcagatg ttccgcgacg atccccgatg
     3241 tgcgcacttc gcggttggaa agttccgggt tctgatgaaa atcccttcgc caggtgatga
     3301 tcttcccatc caccgccttt atttcggcat cgacatcttt ggagtacgct cccggtgaaa
     3361 cccagaacgc cgcgagtgct acccaaggta gcgttttgta ggtgtaagtc gacataggga
     3421 accgagtctg cgctgactgg tcgcagtact gcaacgcccg gatacttcgc aaagctaaga
     3481 aaaatcatca tgcatgttgt cgacgccacc cttttctatt cacccaccag tggtggggtg
     3541 aagcgatacc tgctcgccaa acacgagtgg atgcgcgaac acaccggctg gcgtcacagc
     3601 ctgctcgtcc cgggcgagag ccgcatgcat gcgccgggcg acatatcaac catcgccggt
     3661 ggactcgtcc cgggtgcgtt caactatcgg ctgccgctga atcccgtacg ctggtcgcgc
     3721 gccatcgacg cgctcgagcc cgacctcatc gaagtgggcg acgcgtttca tcccgcctgg
     3781 gcggcggcta acgtcgccaa tcggcgtggc attccgctca tcgccttcta ccattccaac
     3841 tttccgcagc tcgccggaag gcgcctcggc aagtccgtgc agcgcttcat cgagtggtac
     3901 gtgaagctca cctatgaacg ttgtgagcag gtgctcgcgc ccagccgcta catgtgcgat
     3961 tacctgcaca gcatcggcgt cacgcatgcc acctgccaac cgctcggggt cgacgtcgac
     4021 accttcaatc ccgacaagcg tggccgcgac ctggccgcgg aactcaatct gccgcgcggc
     4081 acacgcgtgc tggtgtttgc cggacgcttc tcggccgaaa agaacatccc cgtgcttcac
     4141 gagactttcc gccgtctcgg cgacgactat cacctgctca tgatcggcgg cggcgagtcg
     4201 ggccgcgaag gaaacgtcac gcgtattccc tattgccgtg acaacagcac gctggccagc
     4261 tacttcgcgt cggccgacgc tttcgtacac gccggcgtcc atgaaacatt cgggctcgtg
     4321 gtcgtcgaag ccatggcctg cggccgtccc gtggtggcca tgcgcgccgg tgcattgccg
     4381 gaactggtgg atgcgagcgc gggaaatctg gccgagccgc atgaggaccc aatggtcgcc
     4441 gccgcgaacc tcgcggatgc catcgtccat gcctacgaac gtgacgtcga cgtgctcggc
     4501 gccgcggcgc gccgccacgt gctcgccaac tacagctggt cacggaccct gcagacgttg
     4561 atgacgcgtt accagtccgc ggtcagcgcg cgccgcctgc cgtcgatggc caacggcctc
     4621 gcgggcgccg aaacgaccac ccactagcta gagccagccg ctccatttct gcaggtagtc
     4681 ctgcgcccac ttcacgtcct cgagtcccga gccgacgcga aactggtcga cgtagctcgc
     4741 gaacgtgcgc tgcagcgctt cggccccgcc aaaacgcttc tcgaatttct cgatgacctc
     4801 cggcgggccg ccgatcacgg agtagttcat ctgcgagtcg acgatcagga agacgtcgag
     4861 cgtggatgcg agcaggcagg tgggattcga cacgaggcgg gagcggatcc agcccgcgag
     4921 ttcggcgtcg agttttccgg tgtggcgaaa gaagggtttg ggcgcgctca ggttggcaat
     4981 gtagaactcg ccgccgcaga cgccattgag gaatcccagg aggcgcaccg actcgttgaa
     5041 gctgtcttcg ccgtcgggcg actgaatgcc ttcgggaaat gcgcggccat cgagcggcgc
     5101 tttgggccac cacacttcgt gacggggcct cttcgcctgg ggcgcgaagc gtttgctggc
     5161 cagcatctcg aaacgctgtt gtacctccct gcgttcgtcg ggagtgagat cgcgcagctc
     5221 gaccattacc gtgccaccag ctcgaacagc gtgacgcggc cgttcttttc accggcaacc
     5281 acacgcttgc cgtcgggcga ccatcgcacc acgctgattt ccgaatcgag catctgaacg
     5341 tcgataggct gcttcgcctt ggcgggccgc cacaacgtga ggcgccagtc gcggcccgcg
     5401 ctgacaaagt gttcgctgcc cggctgccag gagaaactgt cgacgcgttc ggtgtggccg
     5461 ctcaacacga tgggacgcgt gccttccgga cccttgccgc tgaaatccca cagcacgact
     5521 tcgttgcctg cactcgaagc gagatagcga ctgttgtcgc tccacgccac cagttcgagc
     5581 ttgccgtcat aaccgcgcat ctgtgaatcc ttgcccgtcg agcgattcca gaagtgcaac
     5641 gtgccgtcgg ccatgccgct agcaaggaat cgcccgttgc cgctgaagtt taccgtgaga
     5701 caggcggcca gccacttgta gcgccgcgtt tcgtagcgcg ctttctcgat gcgatgcacc
     5761 gcgatctcgc cattcagggc aaccccgaga tccgttccag ggcgatcgaa cgacagcgcg
     5821 accgcagtgc tcgccgcggg tgcgaaggcg tggatttttt cgccgagggg cgaccagagc
     5881 gtgaccactt tgcccgcggc gctcgccagg atttcaccgt cgggcgaaaa gctcaacgcc
     5941 tgggtcggtg tgggcgcggg tttccaccgc ttgatctctt tgccggacgt gccatcccag
     6001 agcgccacgg acccgtcctg gccagatgtt gcgaacacgt tcgcgttggg gcgccacacg
     6061 atgctcagcg cgcctaacag gtgttcgccg agcacctcga ccgtcagcga atcggcttcc
     6121 gtgcgcgcca gcgcgacctt gccctcaccg ccggcgatgg cgaacgactt gctgtcgggc
     6181 gcccacgcga tgtcggcgac atagtcgtcg atgctggtgt tcgcacgcgg ttccagcgcg
     6241 aaagacaggg acttcttcga ggccatggct atttcgcgac agcggattcg aaaccggctt
     6301 cgagctcggc gcgatcgagg ttacggccta tgaacacgag gctgttcacg cgcggcgtgg
     6361 ccccccaggg ccgctccgcc tgcgcatcga acatcatgtg cacgccgtgg aaaacctggc
     6421 ggcgatcttc gcccttcacg ttcagcacgc ccttcatgcg gaagatgtcc tggccgcgag
     6481 tcttgagcag atagtcgaac cactgattga ccttcaccgg atcgagcgcg cgtgggtcgc
     6541 tgattccgat ggagctgatg tcgtcctcgt gatggtgcga ggcgaacttg cgctcggccg
     6601 cgggccgctg gccggcgagg tgcaacccga actcgagcgg gtgatgttca cagaacagcg
     6661 cgtaggcgcc ggccttggct atcgtgagtt tgaaatccgc gcccggatgc gagatgtcga
     6721 tggcgttgtg cgaaggtgct ggctccagcg tcccgtggca gggcaccacc gtcgcggctt
     6781 ccgcaaacgc gcgcaccgcc ggttcaatgg ctgcttccag cgccgccggg gtcgccgccg
     6841 tca
//
LOCUS       DATDEU010000410         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1244489, whole genome shotgun sequence.
ACCESSION   DATDEU010000410 DATDEU010000000
VERSION     DATDEU010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244489"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="modC"
                     /locus_tag="VIV63_09795"
     CDS             complement(<1..776)
                     /gene="modC"
                     /locus_tag="VIV63_09795"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEY6124935.1"
                     /translation="MLRVELRKRRGDFTLDATFSAPTPGVTALFGRSGCGKSTLISLI
                     AGLLAPDAGRVVLDDDVLFDSDRHLSVDARHRRIGVVFQDARLFPHLSVRGNLDYGAR
                     RVARETPRPVTFDDVVGLLGLAPLIARRPHELSGGEKQRVALGRALLSQPRLLLLDEP
                     MASLDLARREEVLPYLERLRDSFAIPIVYVSHQFDEVLRLATRVVLLESGRTVADGDI
                     ATVSRDPQLRAIVGPDSVGAVLSGHVERVDATGLASLRVG"
     gene            complement(776..1462)
                     /gene="modB"
                     /locus_tag="VIV63_09800"
     CDS             complement(776..1462)
                     /gene="modB"
                     /locus_tag="VIV63_09800"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006939.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY6124936.1"
                     /translation="MLSAAESEALWLSLSVAARSVAFNLPFAILVAWLLTRTRFAGRT
                     LFDAFVHLPLVLPPVVIGYLLLVLFGVRGPIGSWLNETFGIRLAFTSTGAALATAVMS
                     FPLVVRAIRISLENVDRGLEEAARTLGAGPVDRFLSVTLPLIAPGILAGAVTAFAAGL
                     GEFGAVITFAANIPGETRTLPLALYTALQTPDGDALAARLAMISLSLGLAGLLVSEYL
                     SRVLRRRMGR"
     gene            complement(1499..>2285)
                     /locus_tag="VIV63_09805"
     CDS             complement(1499..>2285)
                     /locus_tag="VIV63_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEY6124937.1"
                     /translation="DARNGHQRPKIEDYGDSLFAVLHIVEPEGAEFRVGEVAIFAGHN
                     YVVTVRSHAERGFQDVRARCEREPDLLRIGSGFVLYALIDAVVDRYFPVLDQLEAELE
                     SVEEKIFADGSPRANIESLYALKKRLMIVKHAVAPLLEGVSNLSGARVPVICGGLREY
                     FRDIYDHLLRLNQTIESIRDSVATAISVNLSMITLQENETMKRLAAYAALIAVPTMVA
                     GIYGMNFEHMPELGWTFGYPGALGVMIVIDICLFYRLRKAKWL"
BASE COUNT          446 a          736 c          771 g          332 t
ORIGIN      
        1 tcgcccacgc gcaaggatgc gagcccggtc gcatcgacac gctccacgtg tccggacagc
       61 acggcgccga ccgaatccgg acccacgatc gcgcgcagct gcggatcgcg actcacggtc
      121 gcgatgtcgc cgtcggcgac cgttcggccc gactccagca acaccacgcg cgtcgccagc
      181 cgcagcactt cgtcgaactg gtgactgaca tagacgatgg gaatggcaaa cgagtcgcga
      241 agccgttcga ggtacggcag cacttcttcg cgacgcgcca ggtccagcga cgccatcggc
      301 tcgtcgagca gcagcagccg cggttgcgag agcagagcgc gaccgagcgc cacgcgttgc
      361 ttttcgccgc cggacaattc gtgcgggcgg cgggcgatga gcggggcaag ccctaacaac
      421 ccgacgacgt cgtcgaaggt gaccgggcgc ggcgtttcac gcgcgacccg gcgggcgccg
      481 tagtcgagat tgccgcgcac cgacaggtgc ggaaacaggc gcgcgtcctg gaagacgacg
      541 ccgatacgcc ggtgccgggc atccacggac agatggcgat cgctgtcgaa caggacgtcg
      601 tcgtccagca ccacgcgccc ggcgtcgggc gcgagaagtc ccgcgatcag cgatatcagc
      661 gtggatttgc cgcagcccga gcggccgaag agcgcggtga ctccgggcgt gggcgcggag
      721 aaggtggcgt cgagcgtaaa gtcgccacgg cgtttgcgga gttcgacgcg cagcatcagc
      781 ggcccatccg gcgtcgaagc acgcggctca gatactcgga gacgagcaga ccggcaaggc
      841 cgagcgacaa cgagatcatg gcgagccgcg ccgccagcgc gtcgccatcg ggggtctgga
      901 gcgcggtata caacgcgagc ggcagcgtgc gtgtttcgcc ggggatgttc gctgcgaagg
      961 tgatgaccgc gccaaactct ccgaggccgg ctgcaaacgc cgtgacggcg ccggccaata
     1021 tgccgggcgc gatcagcggc aatgtcaccg agaggaagcg gtcgaccggg ccggcgccga
     1081 gcgtgcgcgc cgcttcttcg aggccgcggt cgacgttctc gagcgagatg cggatggcgc
     1141 gcacgaccag cggaaacgac atgaccgccg tggccagcgc ggcgcccgtg ctggtgaacg
     1201 cgagccggat gccgaaagtc tcattgagcc agctgccgat ggggccgcgt acgccaaaca
     1261 acacgagcag cagatagccg atcacgacgg gcggcaacac cagtggcagg tgcacgaacg
     1321 cgtcgaacaa cgtgcggccg gcgaatcgcg tgcgcgtgag cagccaggcc accaggatgg
     1381 cgaacggcag gttgaaggca acgctgcgcg ccgccactga aagactgagc cagagtgctt
     1441 cggattcggc cgctgaaagc atggcggcag tatatgacgc cggggcttgc gggcagcctt
     1501 acaaccactt ggccttgcgc agccgataaa acaggcagat gtcgatcacg atcatcacgc
     1561 ccaacgcgcc gggatatccg aaagtccagc caagctcggg catgtgttcg aagttcattc
     1621 cgtatattcc ggcgaccatg gtgggcacgg cgatgagcgc ggcgtaagcc gccaaccgct
     1681 tcatcgtctc gttctcctgc aaggtgatca tcgacaggtt gaccgagatt gcagtggcga
     1741 ccgagtcccg gatcgactcg atggtctggt tgaggcgcag caggtgatcg tagatatcgc
     1801 ggaaatattc acgcaacccg ccacagatca ccgggacccg cgcgccggac aggttcgaga
     1861 cgccttcgag cagcggcgcg accgcgtgct tgacgatcat caggcgcttc ttgagcgcgt
     1921 acaacgactc gatgttggcg cgcggcgaac cgtcggcaaa gatcttttcc tcgactgact
     1981 cgagctcggc ttccagctgg tcgagcaccg ggaagtatcg gtcgaccacg gcatcgatca
     2041 gtgcatacag cacgaatccg gaaccgatgc gcagcagatc tggttcgcgt tcacatcgcg
     2101 cgcgcacgtc ctggaagcca cgctcggcgt ggctgcgcac cgtcaccacg tagttgtgcc
     2161 cggcaaagat cgccacttcg ccgacgcgaa actccgcgcc ctcgggttcg acgatgtgca
     2221 gcaccgcgaa caggctgtcg ccgtagtcct cgatcttcgg gcgttggtgc ccattgcgcg
     2281 catct
//
LOCUS       DATDEU010000411         5253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2119801, whole genome shotgun sequence.
ACCESSION   DATDEU010000411 DATDEU010000000
VERSION     DATDEU010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119801"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2969
                     /locus_tag="VIV63_09810"
     CDS             <1..2969
                     /locus_tag="VIV63_09810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6124938.1"
                     /translation="PPEQRPTWIEQLPATHDTLKPALRKLLAMPPAGNNTYTLSDVSQ
                     QVHIALQGAAGAAESLEFKEGARVGPYELIRELGRGGMGTVWLARRTEGLTRRVVALK
                     LPHPGMLHAEFAARMGRERDILESLAHPNIARLYDAGVTPAGQPYLALEYVEGVPLNV
                     YCDNKRLGVRERLQLFAQVLQAIQHAHSHLVIHRDLKPANILVTDVGVAMVLDFGIAK
                     LTVEGSTAETALTQFGGRALTPDYASPEQIAGQPLTTASDVYSLAVILYELLTGERPY
                     KLTRGSAAALEEAILASDVRRPSQAVSDDTKAALRASTAKKLARALRGDLDTIVLTAM
                     RVKLAERFRTVDAFALDIEQYLAGRAISARPESWWEGARRFVVRHKLAVGSAAVVVLA
                     LTGGIAATTWQAAKARASEQRAIAAAATSEAAKDFMVSIFLTNTLRQEDAAKARSMNA
                     LQLLEAGANELATQFKDNPVVHRELLTVIVRLLGESRSSEYKKHAAELIALLEKFPNT
                     EVERVDLYNELSIMEQGKDPKAGMDYALKGLSILGPSNDPPHRKARAAMLFAISTSKM
                     QSGDVEGALPPLLEAKKLLEDGFTETAEYGHALGNLGWLEMRKDNTAAAVDLFERAMR
                     AYKADPTSYKRTIAQGHGELSIGYTMRKQYAQAEHELRQAAELYRKDYGDDDPETALA
                     TARAGKAVAQQNRFDEAITMLRSAIVILDKPSPNAVPDYIVAAHEYLADTLVQAGRLT
                     EAEPEVKRAIELSSKGGAVSQINPLLIAGDLDAMRGRYASAEKNARAAVALAEKAFGP
                     SAKRTYRTRNRLGRVLVTSGKLDEADALFTANIQADAANAGALDSPWMAASVQHAKVL
                     ILRGKASEAVPTLAAALEKNLAQPVQQQDPNDEIEYQFALGRALNAAGRAQEALPHLE
                     RTLVLRQTQYANSPRLAEAQIALADCKLTLGDAAAGRALFDKARAIHAANRELSDFYR
                     KPLRDLGARLAGARTN"
     gene            complement(2966..3535)
                     /locus_tag="VIV63_09815"
     CDS             complement(2966..3535)
                     /locus_tag="VIV63_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124939.1"
                     /translation="MKTSPVRPVLTAVWCGICLAACSKPEAASSPTATTQGPTSDPCA
                     LTSDNEVRKVFADAKAGVRDHSLDKYDIATCTWDTPANTFVVQLFTAHGSAEDEVRSR
                     ISGSVDPVKPGAGANIRYDSLAGIGDSAVVAAEKADDQQGILADAAVLGIRRGERMVV
                     LFSRSLIDGDRAATVKGLEALGRDAATRL"
     gene            3685..>5253
                     /locus_tag="VIV63_09820"
     CDS             3685..>5253
                     /locus_tag="VIV63_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124940.1"
                     /translation="MKKSLMPALTCALFFLAGEAVAATFTVTNTNDSGAGSLRQAIVD
                     AVAAPTAAHDNVVVFNIPGAGVHTIRPLSQLPAIKDLTIDGYTQPGSQANTLARGSDA
                     VLTIEIDGSLAGAGTHGLVNEGAVPGAGVPNVTVRGLVINRFGGAGIRVTGPGGAGFP
                     GFVTVRGCYIGTDASGTQARGNGIGIDLGTDAQALIGEPAPDFGGNTVPWPAYRNLIS
                     GNVGAGVKFDSADALSPAFGTVRGAYIGTDAAGTSALGNGGDGIAIGAEGGVGSAGFG
                     SFVYLHDNLVSANMGDGIDTQGIGTQAVGNTIGAGSDGRALGNQGNGAYFHGPSAGNL
                     NAVFTAPGAVGPGVSNNAGAGVLIADTAIVDVSGRFTGNLGLGVDLAPVGPNANDVGD
                     TDTCPNDGLNHPVITSAVSDGVSPTSRIQGTIHSKPNTQIQVLLYLNATCNASGFGEA
                     ARFLSSLPGLFTDASGNAAFDAQLAFPIDTTAFPFVVAQARRFAESTAPLPSMIEVSE
                     YSECFRVTGGAALPT"
BASE COUNT          927 a         1744 c         1763 g          819 t
ORIGIN      
        1 tgccgccgga gcagcggccg acctggatcg aacaacttcc cgcaacgcac gacacgctga
       61 agccggcgct gcgcaagctg ctggcgatgc cacccgccgg caacaacacc tacacgctga
      121 gcgacgtcag ccagcaggtg cacatcgccc tgcaaggcgc cgccggcgcg gcggaatcgc
      181 tggagttcaa ggaaggcgcg cgcgtcgggc cgtatgaact cattcgcgag ctcggccgcg
      241 gcggcatggg cacggtgtgg ctcgcgcggc gcaccgaagg tctcacgcgc cgcgtggtgg
      301 cgctcaaact acctcacccc ggcatgttgc acgcggagtt tgcggcgcgc atgggacgcg
      361 agcgcgatat cctcgaaagc ctcgcgcatc cgaacatcgc gcggctctac gacgcgggcg
      421 tcacgccggc cgggcagccc tacctggcgc tcgagtatgt cgaaggcgtg ccgctcaacg
      481 tgtactgcga caacaaacgt ctcggtgttc gtgagcgtct ccagcttttt gcgcaggtgc
      541 tccaggcgat tcagcacgcg cacagtcacc tggtcatcca ccgcgatctg aagcccgcga
      601 acatcctcgt caccgacgtc ggcgtcgcga tggtgctcga cttcggcatc gccaagctca
      661 cggtggaagg ctccaccgcc gaaacggcgc tgacgcagtt cggcggccgc gcactgacgc
      721 cggactacgc gtcgcccgag cagatcgccg gccagccgct caccacggca tcggatgtct
      781 attcattggc tgtgatcctc tacgagctgc tcaccggcga acgcccgtac aaactcaccc
      841 gcggttccgc cgccgcgctc gaagaagcga tcctcgcctc cgacgtgcgc cggcccagcc
      901 aggccgtgag cgatgacacg aaggcggcgc tgcgtgcgtc aaccgcgaag aaactcgcgc
      961 gcgcgttgcg tggcgatctc gacaccatcg tactcaccgc catgcgagtg aagctggcgg
     1021 aacgttttcg aacggtggac gcgttcgcgc tcgacatcga gcaatacctc gccgggcggg
     1081 ccatcagcgc gcgtccggaa agctggtggg aaggtgcgcg gcgattcgtg gtgcggcaca
     1141 agctcgcggt gggcagcgca gccgtggtgg tgctggcgct cacgggcggc atcgcggcga
     1201 cgacctggca ggccgccaaa gcgcgcgcca gcgagcagcg cgccattgcc gcggcggcga
     1261 cctcggaagc ggccaaggat ttcatggtca gcatcttcct gaccaacacg cttcgacagg
     1321 aagacgcggc caaggcgcgt tcgatgaacg cgctgcagtt gctggaagcc ggcgccaacg
     1381 agctggccac gcagttcaag gacaacccgg tcgtgcaccg ggaactgctg acggtgatcg
     1441 tgcggttgtt aggcgaatcg cgttcgtcgg agtacaagaa gcacgcggcc gagctcatcg
     1501 cgctgctcga gaagtttccg aacaccgagg tggagcgcgt cgatctctac aacgaactgt
     1561 cgatcatgga acagggcaag gatccgaagg ccggcatgga ctacgcgctg aagggcctca
     1621 gcatcctggg cccctcgaac gatccgcccc atcgcaaggc ccgcgccgcg atgctcttcg
     1681 cgatctccac gtcgaagatg caatcgggcg atgtcgaggg cgcgctgccg ccgttgctgg
     1741 aagcgaagaa actcctcgaa gacggcttca cggaaacggc cgagtacggc cacgcgctcg
     1801 gcaacctggg ctggctggag atgcgcaagg acaacaccgc ggccgcggtc gatctgttcg
     1861 agcgcgcgat gcgtgcgtac aaggcggacc cgacgtctta caaacgcacc atcgcgcagg
     1921 gccacggcga gctgtcgatc ggttacacga tgcgcaaaca atacgcgcag gccgagcacg
     1981 agttgcgcca ggcggcggag ctgtatcgca aggattatgg cgacgacgat cccgagaccg
     2041 cgctggccac ggcgcgcgcg ggcaaggccg tcgcgcagca aaaccgcttc gacgaagcga
     2101 tcaccatgct ccgctcggcg atcgtgattc tcgacaagcc ttcacccaat gccgtgccgg
     2161 actacatcgt ggcagcccac gagtatctcg ccgacacgct ggtgcaggcg gggcgcctga
     2221 ccgaggcgga gcccgaagtg aagcgggcta tcgaactatc cagcaagggt ggcgcggtct
     2281 cccagatcaa tcctctgctg atcgccggcg acctggacgc catgcgcggc cgatatgcca
     2341 gcgcggagaa aaatgcgcgc gccgcggtcg cgttggccga gaaggcgttt ggcccgagcg
     2401 ccaagcgcac ctatcgcacg cggaaccggc tcgggcgggt gctcgtcacg tccgggaaac
     2461 tcgacgaggc ggacgcgctg ttcacggcga atatccaggc cgacgcggcc aacgctggcg
     2521 cgctcgattc accgtggatg gccgcgagcg tgcaacacgc gaaggtgctg atcctgcgcg
     2581 gcaaagccag cgaggcggtg ccgacgcttg ccgccgcgct cgagaagaac ctcgcgcagc
     2641 ccgtgcagca gcaggatccc aacgacgaga tcgagtacca gttcgcgctg ggccgcgcgt
     2701 tgaacgccgc gggccgcgcg caggaagccc tgccccatct cgagcgcacg ctggtgctgc
     2761 gtcaaacgca gtatgcgaac agtccgcggc tggccgaggc gcagatcgcg ctcgcggatt
     2821 gcaagctcac gctcggcgat gccgcggcgg gtcgcgcctt gttcgacaag gccagggcga
     2881 tccacgccgc gaaccgcgag ctcagcgact tctaccggaa gccgttgcgc gatctcggcg
     2941 cccgcctggc gggcgccagg actaactaga gtcgcgtggc cgcgtcgcgg cccagcgctt
     3001 caagcccttt cactgtggcg gcacgatcgc cgtcgatcag cgaacggctg aacaacacca
     3061 ccatgcgttc cccgcgccgg attccgagca ccgccgcatc ggccaggatg ccctgctggt
     3121 catcggcctt ttccgcggcg acgacggccg aatcgccgat gccggcgagc gagtcataac
     3181 ggatgttggc gcccgcgccc ggcttcaccg gatcgacgga gccggatatg cggctgcgca
     3241 cttcgtcttc ggccgacccg tgcgcggtga agagctgcac gacgaaggtg ttcgcgggcg
     3301 tgtcccaggt gcaggtcgcg atgtcgtact tgtcgaggct gtgatcccgc acgcctgctt
     3361 ttgcatcggc gaaaaccttg cggacttcgt tgtccgaggt cagcgcgcaa ggatcgctgg
     3421 tcgggccctg cgtggtggcc gtggggctcg atgccgcttc cggcttcgaa caagcggcca
     3481 ggcagatgcc acaccacacg gcggtgagta cggggcgaac gggcgaagtt ttcatggggc
     3541 atctcctggc cggtgaagaa acggcggtcc ttgaggggca tacgaaaaag ccgccttgcc
     3601 gcggacatat cgttgtccgc gagaggcgtg cttccgcgta ttccctgatg aggcagccaa
     3661 gtccggctgc gggaaggagt ccccgtgaag aagagcctga tgcccgcgct gacctgcgcc
     3721 ctgtttttcc tggccggcga ggccgtcgcc gccactttca cggtaaccaa caccaacgac
     3781 agcggcgcgg gctcgctgcg ccaggccatt gtcgatgcgg tcgccgcacc gacggcggcg
     3841 catgacaacg tggtggtgtt caacattccg ggcgccgggg ttcacacgat ccggccgctg
     3901 tcccaactac cggcgatcaa ggacctgacg atcgacggct acacgcagcc gggttcgcag
     3961 gcgaacacgc tcgcgcgcgg tagcgacgcg gtgctgacca tcgagatcga cggtagcctg
     4021 gccggcgctg gtacacacgg cctcgtcaat gagggcgccg ttcccggcgc cggggttccg
     4081 aacgtcacgg tgcgcgggct cgtcatcaat cgatttggcg gcgcgggcat tcgcgtgacc
     4141 ggtccgggag gcgcgggctt tccgggtttc gtgacggtgc gaggttgcta catcggcact
     4201 gacgcgagcg gcacgcaagc ccgcggcaat ggcatcggta tcgatctcgg cacggacgcg
     4261 caggcgttga ttggcgagcc ggcgccggac ttcggcggca acaccgtgcc gtggccggcc
     4321 tatcgcaatc tcatctcggg caatgtcggc gcgggcgtga agttcgattc cgccgatgcg
     4381 ctgtctcccg cattcggcac cgtgcgtggc gcctacatcg gcacggatgc ggccggtacg
     4441 tcggcgctcg gcaatggcgg cgacggtatt gcaatcggcg ccgagggcgg cgtgggcagc
     4501 gccgggttcg gcagcttcgt ctatctacac gacaacctcg tctccgcgaa catgggagac
     4561 ggaatcgaca cgcagggcat tggtacgcag gctgtgggca acaccattgg cgccggcagc
     4621 gacggtcgcg cactcggcaa ccagggtaac ggcgcgtact ttcatgggcc aagcgccggc
     4681 aatctgaatg cggtgttcac ggcaccaggt gcggtggggc caggcgtttc gaacaacgcc
     4741 ggcgccggcg tgctgattgc tgataccgcc atcgtcgacg tgtcgggacg cttcaccggt
     4801 aacctgggac tgggcgtcga tctcgcgcct gtggggccga acgcgaatga cgtcggcgat
     4861 accgacacct gccccaacga cggcctgaac caccccgtca tcacttcggc ggtatcggac
     4921 ggagtaagcc cgacgagccg tatccagggg actatccact cgaagcccaa tacacagatc
     4981 caggtgcttc tgtacctgaa tgccacgtgc aacgcgtcgg ggttcggcga agccgcgcga
     5041 ttcctgagtt cgcttcctgg cctcttcacc gacgcgtcgg gtaatgcggc cttcgatgcg
     5101 caactcgcct ttcccatcga caccacggcg ttccctttcg tcgtcgcgca ggcgcgccga
     5161 ttcgcggaga gcaccgcgcc gctgccgtcg atgatcgagg tgtccgagta ctcggaatgc
     5221 tttcgcgtaa ccggcggcgc ggctctgccc acg
//
LOCUS       DATDEU010000412         4590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_77497, whole genome shotgun sequence.
ACCESSION   DATDEU010000412 DATDEU010000000
VERSION     DATDEU010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77497"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VIV63_09825"
     CDS             <1..779
                     /locus_tag="VIV63_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6124941.1"
                     /translation="LETFSGQIGLALERERLSSQAETARVDAQGQSLRSTLLASISHD
                     LRTPLAVMAGAASTLAEHGQSLDETTRMTLARSIEQRAREMSELISNVLDLTRFEAGE
                     LALRRDWESVDDLAAVAIDRLREPLARHAVDLRLPGDLPPVFVDASLAVQLFANLFDN
                     AAKYTPAGTHVTVSALLDGNFLHVFVDDDGPGLPGGDPERLFDKFQRGDGEGAVAGVG
                     LGLAICRAIARAHGGDIKAMPRPGGGARFEFTLPTVEPVR"
     gene            776..1483
                     /locus_tag="VIV63_09830"
     CDS             776..1483
                     /locus_tag="VIV63_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124942.1"
                     /translation="MTNAMHQVLVVEDEPGIRNVLAVLLSAANYRVVEAESAARGEIE
                     ARAHKPDLMIVDLGLPDRDGLELIRRVRSWTAVPIIVLSARSGEDQKIAALDAGADDY
                     VTKPFAAAELLARVRAALRRTALGVLPSSEIRLGNIVIDLARRELRGAGAGIHLTPLE
                     FRVLENLARHAGTIVKQERLIREVWGPEQVDDTRSLRVCVRNLRAKLEPDPRQPRYLV
                     TEAGLGYRLRLDEVPAV"
     gene            complement(1490..3256)
                     /gene="ybaL"
                     /locus_tag="VIV63_09835"
     CDS             complement(1490..3256)
                     /gene="ybaL"
                     /locus_tag="VIV63_09835"
                     /inference="COORDINATES: protein motif:HMM:NF007950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEY6124943.1"
                     /translation="MPAELHETSLIALLAVGFVLALLFGLGASILKVSPIVGYLIAGI
                     CVGPFTPGFVADSGLANQLAEIGVILLMFGVGLHFSIKDLMSVRYIALPGAVAQIATA
                     TAIGVGMAHLLGWNFGTGLVFGLSLSVASTVVLLRALEERNALQSSEGKIAVGWLIVE
                     DLAMVLALVMLPALADTLGGDSSAVNAHTGSGNTWLALAFTLAKVVVFVVLALVVGTR
                     VVPWLLKRVSRTGSRELFTLCVLAIALGIAYGSAAAFGVSFALGAFFAGVILSESELA
                     HQAGNDSLPLKDAFAVLFFVSVGMLFDPTVLVREPVAVISVLAIIMVGKSVAAFIIVL
                     VFRHPLRTALTVSASLAQIGEFSFILASLGAALGLLPPDGRDLILAGALLSIALNPLT
                     FAGIAPLTRWFEARPKLLRLLERSGADRHRRPVSSAEGLRNHAILVGYGRVGGAIGPV
                     LREQGLAFAVIERDHIMLSNAQVQGIPTIEGDAAAPGVLNAAGIETARLLVVATPDSF
                     LARRIVEVARQLNPAIDVVVRTHSESEFKALEGLGASRVVMGERELARGMLEYSLRSL
                     GVPRERAQALAGRDVQLNPEGT"
     gene            3308..>4590
                     /locus_tag="VIV63_09840"
     CDS             3308..>4590
                     /locus_tag="VIV63_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY6124944.1"
                     /translation="MRASFMKAQQALAPAFIGAIGVVFGDIGTSPLYAFKESLIAAGF
                     QPGDTATVLGLLSLIFWSLAIVVSVKYLAVIMRADNHGEGGILSLVALVQQRFGSAEP
                     WARRAVALGVLGAALFYCDALITPAISVLSAVEGLEILNPDLSRAVIPVTLGVIAVLF
                     LIQRRGTEKVGRMFGPIMILWFVVLAVLGAIEIVRAPQVLAAVNPWYALHLFGAHPGL
                     SLVILGAVFLVVTGAEALYADMGHFGRKPVAMAWLVLVWPALLINYFGQGALLMQSAT
                     PIANPFYSLAPQALLPALVLLSTAATVIASQATISGAFSVTREAVQLDLLPRVRVLQT
                     SAEAHGQIYVPAANLFMFVAVVLFVVAFGSSSALSAAYGASVVGTMLITTLLGALVAA
                     SLWNWPVWRVAALFGLMLLVDLAFVAGNATKIASGG"
BASE COUNT          763 a         1511 c         1533 g          783 t
ORIGIN      
        1 tgctcgagac attctcgggg cagatcggcc ttgccctgga acgcgagcgg ctgtcttcgc
       61 aggcggagac cgcgcgcgtg gacgcgcagg gtcagagcct gcgcagcacc ttgctcgcat
      121 ccatctctca tgacctgcgc acgccgctcg cggtgatggc aggcgcggcg agcacgcttg
      181 ccgagcacgg gcaatcgctg gacgagacaa cgcgtatgac gctcgcgcgc agcatcgaac
      241 aacgcgcgcg ggagatgtcc gaactcatct cgaatgttct cgacctcacg cgcttcgagg
      301 cgggggagct cgcgcttcgc cgtgactggg aatccgtcga cgaccttgcg gcggtggcga
      361 tcgaccgcct gcgcgaaccg ctcgcacgcc atgcagtcga cctgcggctg cccggcgatc
      421 tgccgccggt gttcgtcgat gcgagcctcg cggtgcagct gtttgcgaat ctgttcgaca
      481 acgcagccaa gtacacgccc gccggcaccc atgtgacggt ctcggcactc ctcgacggaa
      541 acttcctgca cgtgttcgtc gacgacgatg gcccgggact tccgggaggt gatccggagc
      601 gtttgttcga caagtttcag cgcggtgacg gcgagggtgc cgtcgcgggt gttggtctgg
      661 gacttgccat ctgccgcgcg atagcgcgcg cacacggcgg ggacatcaag gcgatgccgc
      721 ggccgggcgg cggcgcccgg ttcgaattca cgttgcccac cgtggagccc gtgcgttgac
      781 aaatgcaatg caccaggtgc tggtcgtcga ggatgagccg ggcattcgca acgtgctcgc
      841 cgtcctcctg tcggccgcaa actaccgcgt ggtcgaggcg gaatccgcgg cgcgcggcga
      901 gatagaagcc cgcgcccaca aacccgacct gatgatcgtc gacctcggcc tgccggatcg
      961 cgatggactc gaattgatcc gccgcgtgcg ctcctggacc gcggtgccga tcatcgttct
     1021 gtccgcgcgc tccggtgaag accagaagat cgccgcgctc gacgccggcg ccgatgacta
     1081 tgtgaccaaa cctttcgccg ccgcggagct gctggcgcgc gtgcgcgcgg cgctgcgtcg
     1141 caccgccttg ggcgtgctgc cttcttcgga aatccggctc ggcaacatcg tgatcgacct
     1201 ggcgcgccgc gaattgcggg gcgcgggggc cggcatccat ctcacgccgc tcgagttccg
     1261 agtgctcgag aatcttgcac ggcacgcggg aaccatcgtc aagcaggaac gcctgattcg
     1321 agaggtatgg ggccccgagc aggtcgatga cactcgcagt ctgcgggtct gcgtgcgcaa
     1381 tctgcgcgcc aagctcgagc ccgatccacg ccagccaaga tatctcgtca ccgaagccgg
     1441 attgggctat cgattgcggc tggatgaagt cccggccgtc tgaagtattt caagtgcctt
     1501 cggggttcaa ctgaacgtca cgacccgcca gcgcctgtgc gcgctcgcgc ggaactccaa
     1561 ggctgcgcaa cgaatattcg agcatgccgc gcgcgagttc tcgttcgccc atcaccacgc
     1621 ggctggcgcc gagaccttcg agcgccttga attcgctttc cgaatgtgtt cgcacgacga
     1681 cgtcgatggc gggattcaac tgccgcgcca cttcgacgat gcgccgcgcc aggaagctgt
     1741 cgggcgtggc aaccaccagc agccgcgcgg tttcgatgcc cgcggcgttc aggacgccgg
     1801 gcgctgccgc atcaccttcg atggtgggaa ttccctgtac ctgagcgttg ctcagcatga
     1861 tgtgatcgcg ctcgatgacc gcgaacgcca ggccctgttc ccggagcacg ggtccaatag
     1921 cgccgcccac gcgtccatag cccacgagga tggcgtgatt gcgcagtcct tcagcactgc
     1981 tgacgggcct gcggtgccgg tccgcgccac tccgctccag gaggcgcagt agtttgggac
     2041 gcgcttcgaa ccagcgggtg agcggcgcga tgcccgcgaa cgtgagcgga ttgagcgcga
     2101 tggacagcaa tgcgccggcg aggatcaggt cgcggccatc gggcggcagc aggccgagcg
     2161 ctgcgcccag gctcgcgagg atgaacgaga actcgccgat ctgggccagg ctggcggaca
     2221 ccgtcagcgc ggtgcgcagc ggatggcgga agacgagcac gatgatgaac gccgcgaccg
     2281 acttgcccac catgatgatg gccagcaccg agatcaccgc gaccggttcg cgcaccagta
     2341 cggtcgggtc gaacagcatt cccaccgaca cgaagaacaa caccgcgaag gcgtccttga
     2401 gcggcagcga atcgttgccg gcctggtggg cgagctccga ttcggaaagg atgaccccgg
     2461 cgaaaaacgc gccgagcgcg aatgacacgc cgaacgcggc ggcggagccg tacgcgatgc
     2521 cgagcgcgat ggcgagcacg cacagcgtga acagttcgcg cgagccggtg cgcgagacgc
     2581 gcttcagcag ccagggcacg acgcgtgtgc cgaccaccag cgcgagcacg acgaacacca
     2641 cgaccttggc gagcgtgaac gccagcgcca gccaggtgtt cccggaaccg gtatgcgcgt
     2701 tgaccgcgct cgagtcgccg ccgagcgtgt cggcgagcgc cggcagcatc accagcgcca
     2761 gcaccatggc caggtcttcg acgatgagcc agccgacggc gatcttgcct tccgaggact
     2821 gcaacgcgtt gcgctcttcg agtgcgcgca gcagcaccac ggtgctcgcc acggacagcg
     2881 acagtccgaa caccagtccg gtgccgaaat tccatccgag caggtgcgcc atgccgacac
     2941 cgatcgcggt ggcggtggcg atctgcgcca ccgcgccggg cagtgcgata tagcgcaccg
     3001 acatcaggtc cttgatcgag aagtgcaacc cgacgccgaa catcagcagg atgacgccaa
     3061 tttccgcgag ctgattggcg aggcccgagt ccgccacgaa acccggcgta aagggtccta
     3121 cgcagattcc ggcgatgagg tagcccacga tcggggagac cttgaggatg ctggccccca
     3181 atccgaacaa caatgcaagt acgaagccga cggccaggag cgcgatgagc gaagtttcgt
     3241 gtaattccgc gggcatacgc gcgagggtac ccgaaaaatc acgaaatgcg gcgctgcggg
     3301 gcctagaatg cgcgcctcgt ttatgaaggc tcagcaagct ctcgctcccg cattcatcgg
     3361 cgccatcggc gtcgtgttcg gtgacatcgg caccagcccg ctctacgcgt tcaaggaatc
     3421 attgatcgcc gcgggatttc agccgggcga cacggccacg gtgttaggcc tgctgtcgct
     3481 gatcttctgg agcctcgcca tcgtcgtcag cgtgaagtac ctcgcggtca tcatgcgcgc
     3541 cgacaatcac ggcgaaggcg gcatcctctc gctcgtcgcg ctcgttcagc aacgcttcgg
     3601 cagtgccgaa ccctgggcgc gccgcgcggt ggcgctcgga gtattaggcg cggcgctgtt
     3661 ctactgcgat gcgctgatca cgccggccat ttccgtgctg agtgccgtcg aaggcctgga
     3721 aatcctgaat cccgatctct cgcgcgccgt cattccggtg acgctgggcg taatcgccgt
     3781 gttgttcctg attcaacggc gcggcacgga gaaggtgggc cgcatgttcg gccccatcat
     3841 gattctctgg ttcgtcgtgc tcgcggtgct cggcgcgatc gagatcgttc gcgcgccgca
     3901 ggtgctggcg gccgtgaacc cgtggtacgc gcttcatctg ttcggcgcgc atccgggact
     3961 ttcgctggtg atactcggcg ccgtgttcct ggtggtcacg ggcgctgagg cgctgtacgc
     4021 ggacatgggc cacttcgggc gcaagccggt cgccatggcg tggctggtgc tggtgtggcc
     4081 cgcactgctc atcaactatt tcggccaggg cgcgttgctg atgcagtcgg cgacgccgat
     4141 cgccaatccg ttttattcgc tcgctccgca ggcattgctg ccggcgctgg tgctgctgtc
     4201 cacggcggcc acggtcatcg cctcgcaggc gacgatttcg ggcgccttct ccgtcacgcg
     4261 cgaagcggtg cagctcgacc tgctgccgcg cgtccgcgtg ctgcagacct cggccgaggc
     4321 gcacgggcag atctacgtgc cggccgcgaa cctgttcatg ttcgtcgcgg tggtcctgtt
     4381 cgtcgtcgcg ttcgggagct cgagcgcgct gtcggccgcc tacggcgcct cggtggtcgg
     4441 caccatgctc atcaccacgt tgttaggcgc cctggtcgcc gcatccctgt ggaactggcc
     4501 ggtgtggcgc gtggcggcgc tgttcgggct catgctgcta gtcgacctgg cgttcgttgc
     4561 gggcaacgcc accaagatcg ccagtggtgg
//
LOCUS       DATDEU010000413         7071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1682992, whole genome shotgun sequence.
ACCESSION   DATDEU010000413 DATDEU010000000
VERSION     DATDEU010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682992"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /gene="dapE"
                     /locus_tag="VIV63_09845"
     CDS             <1..723
                     /gene="dapE"
                     /locus_tag="VIV63_09845"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF009557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY6124945.1"
                     /translation="PSVDGTKKVVETLRERGQKIDWCIVGEPSSEKELGDTIKIGRRG
                     SLSGRLTVHGVQGHIAYPQLAENPVHTFAPALAELTARTWDNGNEHFQPTSFQISNFN
                     AGTGAPNVIPGELKARFNLRYSPVQTIDGLKVIVEDILKKHGVRFTLEWYVSGEPFYT
                     PPGALSAGVTKAIETETGARPKFSTGGGTSDGRFIATLGSQVVECGVINATIHKVNES
                     VNVADIDRLHRIYLNALKEMLA"
     gene            816..1457
                     /locus_tag="VIV63_09850"
     CDS             816..1457
                     /locus_tag="VIV63_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY6124946.1"
                     /translation="MNFISRLMPREGHFFILFDNHAKLIVEGAVSLADLLRHYDTVKD
                     RAAGIKAIEDAEHAADRITHETVQLLHTTFVTPFDRDDIHRLISRMDDVLDLIQDTGE
                     SLVLYDIQKVTPEATQLAELLLRCSERVQSAVGLMASMADAPTILKICQEIDKLESEA
                     DKVMRSAISELFRNETDTRQLIKLKAVYESLESATDKCQDVANVIESVVLENS"
     gene            1477..2478
                     /locus_tag="VIV63_09855"
     CDS             1477..2478
                     /locus_tag="VIV63_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY6124947.1"
                     /translation="MDLGTVSLTLIFLVLLALAFDFMNGFHDAANSIATVVSTGVLKP
                     WQAVLWAAFFNVIAVLFFKLTVAATVGKGIIDPSFVDNRLIFGALVGAISWNLITWYY
                     GIPSSSSHALIGGMIGAALSAAGTAPLLAPGIIKTASFIIVSPMLGMALGALIMLSVS
                     WICFRQTPRRVDKWFRRLQLVSSAAYSLGHGGNDAQKTMGIIWLLLIAGGVTTTDHLP
                     AWVVISCFLAIGFGTMFGGWRIVKTMGQRITKLKPVGGFAAETGGAITLFVATHFGIP
                     VSTTHTITGAIIGVGAAQRSSAVRWGVAGNIVIAWIVTIPASAGIAAVAWWIGGLIF"
     gene            complement(2494..2958)
                     /locus_tag="VIV63_09860"
     CDS             complement(2494..2958)
                     /locus_tag="VIV63_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY6124948.1"
                     /translation="MALDDLDRSLLDLLQKDNRRSLRDYALDLGISTPTCLRRLRRLE
                     KGGVIQRHAAILDPLRAGFAVMAYVEVALVNPSGARMAAFERRMQRCREVLLCAELAG
                     HVDYLLTVVTRDMPEFRDFTRRQLAGDAEVRKFRSLLVLRQSKEGAALPMTG"
     gene            3051..3731
                     /locus_tag="VIV63_09865"
     CDS             3051..3731
                     /locus_tag="VIV63_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY6124949.1"
                     /translation="MTANPPIPNRGERLTSAIEWLAAAAIVLGHNVWGVVPNEVLILT
                     VVAIVSMRLRAARWDFRTLGFRRPRSWRFIVLIALAAAALRLLLSDFVIEPLTSDIWP
                     PQELPDGADELAGNAVNALIALALVWTFAAFGEEIAYRGYLLNRGAEALGAAPLAFWI
                     AAVASAVLFGYGHFYKGPAGVLDSGVAGLILAAAYLMTGRNLWTCILAHGFIDTFAVA
                     WLYLGLPD"
     gene            complement(3728..4234)
                     /locus_tag="VIV63_09870"
     CDS             complement(3728..4234)
                     /locus_tag="VIV63_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY6124950.1"
                     /translation="MFDRRQKINLACALVCAAMLGFAIFYAEKHLGLEPCPLCMFQRV
                     CIGALGIVFLLAGLHRVRYHGAIVYAGFIFVAAAAATWVSGRHVWIQSQPPGTVPACG
                     APLDNLLQMFPLLEVIRKVMTGGGECGNIDWTLLGISMPGWVLISAVTLGLVGVVNNG
                     FYPVRARR"
     gene            complement(4340..5056)
                     /gene="phoU"
                     /locus_tag="VIV63_09875"
     CDS             complement(4340..5056)
                     /gene="phoU"
                     /locus_tag="VIV63_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY6124951.1"
                     /translation="METADLSHHISRRFNEDLERVRNKVLTMGGMVEQQLANAVTALI
                     EGDSNLGVSVARDDFKVNGMEVSIDEECSRILATRSPAAGDLRVIVAIIKTITDLERI
                     GDEGEKIGYIASRLATMERPADKYREIKHLGRIVSEMVHQALDAFARMDSEAALRVAR
                     QDRVVDEEYESIQRQCITFMMEDPRSIRRALDVMWIVRALERIGDHAKNICEYVIYMV
                     HGKDVRHTKLEDIERDLRGK"
     gene            complement(5104..6417)
                     /gene="phoR"
                     /locus_tag="VIV63_09880"
     CDS             complement(5104..6417)
                     /gene="phoR"
                     /locus_tag="VIV63_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY6124952.1"
                     /translation="MTSGATAFVYVTLRIFAALVAGAVLGWLAGSIWLGISGALGLYL
                     AWNLWQLRALHFWLQNRSVADPPDGMGLWGDVVAQVVRLHRRKRFHKEKLTRLFRELR
                     RSTAAMPDGVVMLDPQGEIVWFNRKAGELLDLSRRADLGLRIDNLVRNPDFVRYLRDG
                     IYSMPVIVRIDTPIERHIAFQLISYGEDQRLLMLRDVTREVRMEQMRKDFVANASHEL
                     RSPLTVVAGYLENFSADPGLGELAAPVAEMRRQTDRMTRIIEDLLELSRFEANDAPIK
                     GLPIDVSAMASMLRKDVLARPVHPRQVEVSIESGAQLIGEEAMVQSAFSNLVDNAAKY
                     TPADGSMQIRWWTDEEGGHFSVRDTGPGISTEHLPRLTERFYRVDPGRSRETGGSGLG
                     LAIVKHALHRHGARLEIESIEGRGSTFTCHFPHDRVLERRVAASA"
     gene            complement(6423..>7071)
                     /gene="phoB"
                     /locus_tag="VIV63_09885"
     CDS             complement(6423..>7071)
                     /gene="phoB"
                     /locus_tag="VIV63_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY6124953.1"
                     /translation="EMIAFGLRRAGFEVREAADAQSGRAEVANKRPDLLLVDWMLPDT
                     SGLEFTRALKRDRETRELPVIMLTARAEEGDKVAGLEGGADDYITKPFSPRELLARIN
                     AVLRRTTPVEGTERIDVEGLVLDQSSQRVSSGERTVALGPTEYRMLAFFMTHPERVYT
                     REQLLDRVWGGNVYVEERTIDVHIRRLRKALEEYGYDRLIQTVRGSGYRFSARPL"
BASE COUNT         1240 a         2303 c         2263 g         1265 t
ORIGIN      
        1 ccctcggtcg acggcaccaa gaaggtggtc gagacgctgc gcgaacgcgg tcagaagatc
       61 gactggtgca tcgtcggcga accttcgagt gagaaagaac tcggcgacac gatcaagatc
      121 ggccgtcgcg gctcactctc cggccggctg accgtgcatg gcgtccaggg gcacatcgcc
      181 tatccgcaac tcgccgagaa tccggtgcac acgtttgcac ccgcgctcgc cgaactcacg
      241 gcgcgcacct gggacaacgg caacgaacac ttccagccca ccagcttcca gatctcgaac
      301 ttcaacgctg gtacgggcgc gcccaacgtg attccgggag aactcaaggc gcgattcaac
      361 ctgcgctact cgcccgtaca gaccatcgac ggactcaagg tcatcgtcga agacatcctc
      421 aagaaacacg gtgtgcggtt cacgctcgag tggtacgtgt ccggcgagcc gttctatacg
      481 cctccgggcg cgttgtcggc gggagtcacc aaggccatcg agaccgagac cggcgcgagg
      541 ccgaaattct cgacgggggg cggcacttcg gacggacggt tcatcgcgac tctcggctcc
      601 caggtggtcg agtgcggcgt catcaacgcc accatccaca aggtcaacga gagcgtcaac
      661 gtggccgaca tcgaccggtt gcaccgcatc tatttgaatg cgttgaaaga aatgctcgcc
      721 tgagagggtt ccgaatgcgt ccgtgacgca tttgtgacag cgccgcttcg acccactaca
      781 ctccgcgtcg caaatcggcg gcggaccaaa ccctaatgaa cttcatttcg cgtctcatgc
      841 cccgcgaagg gcatttcttc atcctcttcg acaaccacgc caaactcatc gtcgaaggcg
      901 ccgtgtccct cgccgacctg ctgcgccact acgacacggt caaggatcgc gccgcgggca
      961 tcaaggcgat cgaagacgcc gaacatgcgg ccgaccggat cacgcacgag accgtgcagc
     1021 tgctgcacac gacgttcgtc acccccttcg accgcgatga catccatcgc ctgatctcgc
     1081 gcatggacga cgtgctcgac ctcatccagg acaccggcga atctctggtg ctctacgata
     1141 tccagaaagt gacgcccgaa gcgacgcagc tcgccgagtt gctgctgcgc tgctccgagc
     1201 gcgtgcagtc cgcggtgggt ctcatggctt cgatggccga tgcgccgacc atcctcaaga
     1261 tctgccagga gatcgacaaa ctcgaatccg aagcggacaa ggtcatgcgc agcgcgatct
     1321 ccgagctgtt tcgcaatgaa accgacaccc gtcagctgat caagctgaag gcggtgtacg
     1381 aatcgctcga gtcggccact gacaagtgcc aggacgtggc caacgtcatc gaaagcgtcg
     1441 tgctcgaaaa ctcctgaaag gcacgcagcg tcccacatgg atctcggtac tgtctcactg
     1501 acgctgatct tcctcgtgtt gctcgcgctc gcgttcgatt tcatgaacgg gtttcacgac
     1561 gcggcgaatt ccatcgccac ggtggtttcc accggtgtgt tgaagccctg gcaggcggtg
     1621 ctctgggccg cgttcttcaa tgtgatcgcc gtgctcttct tcaagctcac ggtcgccgcg
     1681 accgtcggca aaggcatcat cgatcccagc ttcgtcgaca accggctcat cttcggcgcg
     1741 ctggtcggcg cgatcagctg gaatctcatc acgtggtatt acggcatccc gtcgagctcc
     1801 tcgcacgcgc tcatcggcgg catgatcggc gcggcgctct ccgccgccgg cacggctccc
     1861 ctgctcgcac ccggcatcat caagaccgct tcgttcatca tcgtgtcgcc catgctcggc
     1921 atggcactcg gtgcgctgat catgctcagc gtctcgtgga tctgtttccg gcagacgccg
     1981 cggcgcgtcg acaaatggtt ccggcgcctt cagctggtct catcggcggc atacagcctg
     2041 gggcatggcg gcaacgacgc gcagaaaacc atgggcatca tctggctgct gctcatcgcg
     2101 ggtggcgtca ccaccactga tcacctgccc gcctgggtcg tcatcagctg cttcctggcg
     2161 atcggcttcg gcaccatgtt cggtggctgg cgcatcgtga agaccatggg acagcgcatc
     2221 accaagctga agcccgtcgg tgggttcgcg gcggaaaccg gcggcgcaat cacgctgttc
     2281 gtcgcgacgc acttcggcat tcccgtctcg accacgcaca ccatcaccgg cgccatcatc
     2341 ggcgtcggcg cggcgcagcg ctcgtcggcc gtgcgctggg gtgtggccgg aaacatcgtg
     2401 atcgcctgga tcgtgacgat ccccgcttcg gctggtatcg ccgcggtcgc ctggtggatt
     2461 ggcggactta tcttctgatt gatcccgccg gcgtcagccg gtcatgggca gcgcggcgcc
     2521 ctccttcgac tgccgcagca ccagcaacga acggaacttg cgcacctcgg cgtcgccggc
     2581 caactggcgc cgcgtgaaat cgcggaactc cggcatgtcg cgcgtcacca cggtcagtag
     2641 atagtcgacg tgcccggcga gctcggcgca gagcagtacc tcgcggcaac gttgcatgcg
     2701 ccgttcgaac gccgccatgc gcgcgcccga aggattcacc agcgccacct cgacgtaggc
     2761 catcacggcg aacccggcgc gcagcggatc gagaatcgcg gcgtggcgct ggatgacgcc
     2821 gcccttctcc aggcgtcgca gccggcggag gcaggtgggc gtggaaatcc cgaggtccag
     2881 cgcgtaatcg cgcaggctgc ggcgattgtc cttctgcagc aggtcgagca gcgaacgatc
     2941 gaggtcgtcg agtgccatgc cggcctccgc gggatccgac gtgaaacgaa tggaaaaact
     3001 gatggcccgg aattgtcacg cgtttcacgc gttcctgcca cgctggcggc gtgacagcca
     3061 acccgccgat cccgaaccgt ggcgaacgtc tgaccagcgc gatcgagtgg ttagcggccg
     3121 cggccatcgt gctgggccac aacgtctggg gtgtcgtgcc caacgaagtt ctgattctca
     3181 ccgtggtggc gatcgtttcc atgcgcctgc gcgccgcgcg ctgggacttc cgcacgctcg
     3241 ggtttcgccg gcctcgttcg tggcggttca tcgtcctgat tgcgctcgcc gcggcggctt
     3301 tgcgcctgct gctctcggac ttcgtgatcg agccgctgac ttcggatatc tggccgccac
     3361 aggaattacc ggacggcgcg gacgaactcg ctggcaatgc ggtcaatgcg ctcatcgcgc
     3421 tggccctggt gtggacgttc gcggctttcg gcgaagagat cgcctatcgc ggctatctgc
     3481 tcaatcgcgg cgctgaggcg ctgggcgccg cgccgctcgc gttctggata gccgccgtgg
     3541 cgagcgcggt gctgttcggc tacggccact tctacaaggg gccggcggga gtactggatt
     3601 cgggggtggc cgggttgatc ctggccgcgg cctatctgat gaccggccgc aacctctgga
     3661 cctgcatcct ggcgcacggc ttcatcgaca ccttcgccgt cgcctggctg tacctcggcc
     3721 tgccggacta gcggcgcgcg cgcaccgggt agaaaccgtt gttgacgacg cccacgaggc
     3781 cgagcgtgac ggctgagatg agcacccagc cgggcatgga gatgccgagc agagtccagt
     3841 cgatattccc gcactcgcca ccaccggtca tgactttgcg aatgacctcg agcagcggga
     3901 acatctgcag caggttgtcg agcggcgcac cgcaggccgg aaccgtgccc ggcggctggg
     3961 actggatcca gacgtggcgg cccgacaccc aggtcgccgc ggcggcagcg acgaagatga
     4021 acccggcgta gacgatcgcg ccgtgataac gcacgcgatg cagcccggcg aggaggaaca
     4081 cgatgccgag ggcgccgatg cagacccgct ggaacatgca gagcggacaa ggctcgaggc
     4141 cgagatgctt ctcggcgtag aaaatcgcga agcccagcat ggcggcgcag acgagtgcgc
     4201 aggcgagatt gatcttctgg cgacggtcga acatggcggc aactataacg agtcgggggc
     4261 aggcgcagcc agcgttacgc catgtcgtcg cgcctcttga ggtccggccc cttttgctag
     4321 gaaatgggga ctgtccccat tattttccgc gcaggtcccg ctcgatgtct tcgagcttgg
     4381 tgtggcgcac gtccttgccg tgaaccatgt aaatcacgta ctcgcagatg ttcttggcgt
     4441 gatcgccgat gcgctcgagc gcgcgcacga tccacatgac gtcgagcgca cgccggatgc
     4501 tgcgcgggtc ctccatcatg aacgtgatgc actggcgctg gatggactcg tactcctcat
     4561 cgaccacgcg gtcctggcgc gcgacgcgca acgcggcctc gctgtccatg cgtgcaaagg
     4621 catcgagcgc ctggtgcacc atctccgaca cgatacgtcc aagatgtttg atctcgcgat
     4681 acttgtccgc gggccgctcc atggtcgcga gacgcgaggc gatgtagccg atcttctcgc
     4741 cctcgtcgcc gatccgctcg aggtcggtga tggtcttgat gatggcgacg atgacacgca
     4801 ggtcaccggc ggccggcgaa cgcgtggcga gaatgcgcga gcattcttcg tcgatggaca
     4861 cttccatgcc gttgaccttg aagtcatcgc gcgccaccga cacgcccagg ttgctgtcgc
     4921 cttcgatgag cgccgtgacg gcattggcga gctgctgctc caccatgcca cccatggtca
     4981 gcaccttgtt gcggacgcgc tccaggtctt cgttgaatcg gcgcgagatg tggtgcgaaa
     5041 gatcggcggt ttccatggtt cctcaggcga agtgtgtgag ggcgcagtga cgggttgatg
     5101 acatcaggcg ctggcggcga cgcgccgctc gagcacgcgg tcgtgcggaa agtggcaggt
     5161 gaaagtgctg ccgcggccct cgatgctctc gatttcgagc ctggcgccgt gacgatgcaa
     5221 tgcgtgtttg acgatggcga gacccagccc cgacccgccc gtctcacgcg agcggccggg
     5281 atcgacgcgg tagaaacgtt ccgtgaggcg cggcaggtgc tcggtcgaaa tgccggggcc
     5341 cgtatcgcgc acggaaaaat gtccgccttc ctcgtccgtc caccagcgga tctgcatgct
     5401 gccgtcggcg ggcgtgtatt tggcggcgtt gtccacgaga ttggagaacg ccgactgcac
     5461 catggcctct tcgccaatga gctgggcgcc cgattcgatg gagacttcga cctgccgtgg
     5521 atgcaccggg cgcgcgagca cgtccttgcg cagcatggac gccatcgccg acacgtcgat
     5581 gggcaaacct ttgatgggtg cgtcgttggc ctcgaagcgc gagagctcca gcaggtcttc
     5641 gatgatgcgc gtcatgcgat ccgtctggcg ccgcatctcg gcgaccggcg ccgcgagttc
     5701 gccgaggccc gggtcggcgc tgaagttctc gagataaccg gccacgacgg tcagcggcga
     5761 acgcagctcg tgtgatgcat tggccacgaa atccttgcgc atctgttcca tgcgcacttc
     5821 gcgcgtgacg tcgcgcagca tgagcagccg ctgatcttcg ccgtacgaga tgagctggaa
     5881 cgcgatgtgc ctttcaatcg gcgtgtcgat gcgcacgatg acgggcattg aatagatgcc
     5941 gtcgcgcagg taacgcacga aatccggatt gcggaccagg ttgtcgatgc gcagccccag
     6001 gtccgcgcgc cgcgagaggt cgagcagttc gccggccttg cggttgaacc agacgatctc
     6061 gccctgcgga tcgagcatga ccacgccgtc cggcatggca gcggtcgaac gccgcagttc
     6121 gcgaaacagc cgagtgagtt tttccttgtg gaaacgcttg cgacgatgca gccgcaccac
     6181 ctgcgccacc acgtcacccc aaaggcccat gccgtcgggc ggatcggcca cgctgcggtt
     6241 ctgcaaccag aagtgaagcg cgcgcagctg ccagaggttc caggcgagat acaggccaag
     6301 cgccccgctg attccgagcc agatcgagcc cgcgagccac ccgagcaccg cgcccgcgac
     6361 cagcgccgcg aatatgcgca gcgtgacgta gacgaaggcc gtggcgcccg aagtcatccg
     6421 cgtcagagag gtcgggccga gaaccgataa cccgaaccgc gtacggtctg gatcagccgg
     6481 tcgtagccgt actcttcgag cgccttgcgc aggcgacgga tgtggacatc gatggtgcgt
     6541 tcttcgacgt agacgttgcc gccccagacg cggtcgagaa gttgttcgcg cgtgtacacg
     6601 cgttccggat gcgtcatgaa aaacgcgagc atgcggtatt cggtgggacc cagcgccacg
     6661 gtgcgttcgc cggaactcac gcgctgactg ctctggtcga gcacgagccc ttcgacgtcg
     6721 atgcgctcgg taccttccac cggcgtggtg cgtctcagca ccgcgttgat ccgggcgagc
     6781 agttcgcgcg gcgagaacgg cttggtgatg tagtcgtcgg cgccgccttc gaggccggct
     6841 accttgtcgc cctcttcggc gcgcgcggtg agcatgataa cgggcagctc gcgggtttcg
     6901 cggtcgcgct tgagcgcgcg cgtgaactcg aggccgctgg tgtccggaag catccagtcg
     6961 accagcagca ggtcgggacg cttgttggct acctcggctc ggcccgattg ggcgtcggcc
     7021 gcctcgcgga cttcgaaccc ggcacggcgc aagccgaatg cgatcatctc g
//
LOCUS       DATDEU010000414         5136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_223647, whole genome shotgun sequence.
ACCESSION   DATDEU010000414 DATDEU010000000
VERSION     DATDEU010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223647"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VIV63_09890"
     CDS             complement(<1..679)
                     /locus_tag="VIV63_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6124954.1"
                     /translation="MKYDYDLFVIGAGSGGVRAARIASLTGAKVAVAEEYRIGGTCVI
                     RGCVPKKFLVYGAEFSQMFADAPGYGWTAGPATFSWPTLRDNVQKEVSRLSAIYTSNL
                     AKAGVTSFEERAEVVDAHTVKLGKSGGELTAERILVATGGRPYLPEGLPGLELGITSN
                     EAFLLEKFPERVLIVGGGYVALEFANIFHGLGANTRLLHRGDKLLRGFDEDLRTHMHI
                     EVERGGTH"
     gene            716..1714
                     /locus_tag="VIV63_09895"
     CDS             716..1714
                     /locus_tag="VIV63_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY6124955.1"
                     /translation="MNTFKAFRIHEASGKISGRFEELTLDALTPGEVVIEVAYSGINY
                     KDALAATGAGRILRRYPLVGGIDLAGRVASSDDPAFKPGDAVLVTGSGLSESFDGGYA
                     EFARVKAESVIAMPVGLDAFRAMALGTAGFTAALSIHRMERNGQSPSRGPIVVTGATG
                     GVGSIAIDMLSARGYETIAVTGKAESIEYLKSLGASQVVLRGDFALGTKPLEPTRYAG
                     AIDSVGGEMLAGLTRQMDFWGNIASVGLAGGAALSTTVLPFILRGVNLLGINSSATPR
                     AERLEVWHRIATDLSPRHLARIAQRTIAFAELPGAFQAYLDGKVTGRTVVKILPQP"
     gene            1724..2629
                     /gene="prpB"
                     /locus_tag="VIV63_09900"
     CDS             1724..2629
                     /gene="prpB"
                     /locus_tag="VIV63_09900"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016142557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY6124956.1"
                     /translation="MNIPARAQASAGAKFRAAIEAEKPLQVVGAINAYVARMAEATGF
                     RALYLSGGGVAANSLGLPDLGISTMDDVLIDIRRITEASTLPLLVDADTGWGGAFNIA
                     RTVKSFTKAGAAGMHIEDQVQSKRCGHRPGKEVVPAQEMVDRVKAAVDARTDESFVIM
                     ARSDALATEGLERTIERLVKCVEAGADAIFPEAITDLPMYRKVRDAVRVPVLANITEF
                     GKTPLYTREELGGVGVDIVLHCCSAYRAMNLAALEVYQAIRRDGTQKNVVGRMQTRDD
                     LYKFLDYHAYEQKLDELFASEREKK"
     gene            2626..3780
                     /gene="prpC"
                     /locus_tag="VIV63_09905"
     CDS             2626..3780
                     /gene="prpC"
                     /locus_tag="VIV63_09905"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEY6124957.1"
                     /translation="MNAPESNIGKPKKSVALSGVAAGNTALCSVGRSGNDLHYRGYDI
                     LEVAETCEFEEIAYLLIHGKLPNVQELAAYKTKLKNLRGIPAPVRAILEQLPPATHPM
                     DVMRTGASALGCVLPEANDHNAAGAREIADHLVASFASMLCYWFHFSHSSRRIHVETD
                     DDSVGGHFLHLLHRRQPSPSWVRAMHTSLNLYAEHEFNSSTFTARVIAGTGSDIYSCI
                     TGAIGALRGPKHGGANEVALEIQERYATPDEAEADIRRRVAAKEIVIGFGHPVYTVSD
                     PRNVIIKRVAEKLGREAGDMKMYNVAERIEGLMGDIKKMFPNLDWYSAVSYHLMGVPT
                     AMFTPLFVLSRVTGWSAHVIEQREDGKIIRPAANYIGPENRKFVPLAERP"
     gene            3801..>5136
                     /locus_tag="VIV63_09910"
     CDS             3801..>5136
                     /locus_tag="VIV63_09910"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEY6124958.1"
                     /translation="MSSHDYKSAQRPNPDELLVAIADYTKNYQVVSAQAYETARYCLM
                     DTLACGFQALKYPACTRLLGPVVPGATMPGGSRVPGTSYELDPVMGAFSIGAMVRWLD
                     FNDTWLAAEWGHPSDNLGAILAVSDYLSRRAILLGEKPLTVRDVLTAMIKAHEIQGVL
                     ALENSFNRVGLDHVLLVRVASTAVATAMLGGSLEQIVNALSNAWIDGGALRTYRHAPN
                     TGSRKSWAAGDATSRAVRHALFALKNEMGYPSALSAKTWGFQDVLFKGKALTLAQPLG
                     SYVMENVLFKISFPAEFHAQTAVECAMALHPKVKDKLALIDRIVIETQEPGVRIIDKT
                     GPLSNPADRDHCIQYMVAVPLIFGRLTAADYEDSIANDPRIDLLRSKMEVRENTTFTK
                     EYYERDKRHIGNALHIFFSDGSPKLELRIDFPVGHRKRRAEGMPLLVQKFESS"
BASE COUNT          971 a         1697 c         1616 g          852 t
ORIGIN      
        1 ggtgcgtgcc gccgcgctcc acttctatgt gcatgtgcgt acgcaggtct tcgtcgaagc
       61 cgcgcagcag cttgtcgccg cgatgcagga ggcgcgtgtt ggcgcccagc ccatggaaaa
      121 tgttcgcgaa ctcgagcgcg acgtagccgc cgccgacgat gagcacgcgc tcggggaatt
      181 tctccagcag aaatgcttcg ttggaggtga tgccgagttc gagtccgggc aatccctccg
      241 gtagataggg ccggccgccg gtcgcaacca ggatccgttc cgcggtgagc tcgccgccgg
      301 attttccgag cttcacggtg tgcgcgtcga cgacttcggc gcgctcttcg aaagacgtga
      361 cgccggcctt cgccagattg gaggtgtaga tcgccgagag ccgcgagact tccttctgca
      421 cgttgtcgcg cagcgtcggc cagctgaacg tggccgggcc cgcggtccat ccgtagcccg
      481 gcgcgtcggc aaacatctgc gagaactccg cgccgtacac gagaaatttc ttgggtacgc
      541 agccgcggat gacgcaggtg ccgccgatgc gatactcctc cgccaccgcc accttcgcgc
      601 cggtcagaga ggcgatgcgc gcggcgcgta cgccgcccga gcccgcgccg atcacgaaca
      661 ggtcgtagtc gtatttcatg gcaggatact agcctgtccg gtacggggag ctgcaatgaa
      721 cacattcaag gcgttccgca tccacgaggc gtccggaaaa atttcggggc gcttcgaaga
      781 gctgacgctg gacgcgctca cgcccggcga agtcgtcatc gaagtcgcgt actccggcat
      841 caactacaag gacgcgctgg cggccaccgg cgcgggaaga atcctgcgtc gctatccact
      901 cgtgggtggc atcgatctcg cgggccgtgt cgcatcgtcc gacgatccgg ctttcaaacc
      961 cggcgatgcg gtactggtca cgggcagcgg gctgtcggag tccttcgatg gcggttacgc
     1021 ggagttcgcg cgcgtcaaag cggaaagtgt cattgcgatg cccgtgggcc tcgatgcctt
     1081 tcgcgcgatg gcgctgggca cggcgggttt caccgcggcg ctgtcgatcc atcgcatgga
     1141 gcgcaatggc cagagtccgt cgcgcggacc catcgtcgtc acgggagcga ccggtggcgt
     1201 cggcagtatc gccatcgaca tgctgtcagc gcgcggttac gaaaccatcg cggtcacggg
     1261 caaagcggaa tccatcgagt acctgaaatc gctgggcgcg agccaggtgg tgctgcgtgg
     1321 cgatttcgcg ctcggcacga agccgctcga gcccacgcga tacgccggcg ccatcgacag
     1381 cgtgggcggc gaaatgctcg cggggctcac gcggcagatg gatttctggg ggaacatcgc
     1441 aagcgtcggg ctggcgggtg gcgccgcgct ttcgaccacc gtcctgccgt tcatcctgcg
     1501 cggcgtgaat ctgctcggca tcaactcctc ggcgacaccg cgcgcggagc gcctggaagt
     1561 gtggcatcga atcgccacgg acctgagtcc ccgccacctc gcacgcattg cgcagcggac
     1621 catcgcgttc gcggagttgc caggagcgtt ccaggcgtac ctcgacggaa aagttaccgg
     1681 ccggactgta gttaagatcc tcccacagcc gtaaacttgc gccatgaata ttcccgctcg
     1741 cgcccaagct tccgcgggcg ccaagttccg tgcggccatc gaggccgaaa aacccctgca
     1801 ggtggtcggc gccatcaatg cctacgtggc gcgcatggcc gaagccaccg ggttccgcgc
     1861 gctctatcta tccggcggtg gcgtcgccgc gaactcgctc ggccttccgg atctcggcat
     1921 cagcaccatg gacgatgtac tcatcgacat ccggcgcatc accgaggcca gcacgctgcc
     1981 cttgttagtc gacgcggaca ccggctgggg cggcgcgttc aacatcgcgc gtacggtcaa
     2041 gtccttcacc aaggccggcg cggccggcat gcacatcgaa gaccaggtgc agtccaagcg
     2101 ttgcggccac cggcccggaa aggaagtggt gcccgcgcag gagatggttg atcgcgtgaa
     2161 agcggccgtc gatgcgcgca ccgacgaaag cttcgtgatc atggcgcgca gcgatgcgct
     2221 ggcgaccgaa ggtctcgaac gcaccatcga gcgcctcgtc aaatgcgtgg aagcgggcgc
     2281 ggatgccatc ttccccgagg ccatcaccga cctgccgatg tatcgcaagg tgcgcgacgc
     2341 ggtgcgcgtg ccggtgctgg ccaacatcac cgagttcggc aagacacctc tctatacgcg
     2401 tgaagaactg ggcggcgtcg gtgtggacat cgtgctgcac tgctgctccg cgtaccgcgc
     2461 catgaacctt gccgcgctcg aggtctacca ggccattcgt cgcgacggca cgcagaaaaa
     2521 cgtcgtgggc cgcatgcaga cgcgcgacga cctctacaaa ttcctcgact atcacgccta
     2581 cgaacagaag ctggatgaac tgttcgcgtc ggagagggag aagaaatgaa cgcgcccgaa
     2641 tccaatatcg gcaagcccaa gaaatccgtc gcgctctcgg gcgtcgccgc cggaaacacc
     2701 gcgctgtgca gcgtgggccg cagtggcaac gacctccact accgcggcta cgacatcctc
     2761 gaagtggccg agacctgcga gttcgaggag atcgcctatc tattgatcca cggcaagctc
     2821 ccgaacgtac aggagctcgc cgcgtacaag acgaagctca agaacctgcg cggcattccg
     2881 gcgccggtcc gcgccatcct cgaacagttg cccccggcca cccatcccat ggacgtcatg
     2941 cgcaccggcg catcggcgct gggctgcgta ttgcccgaag ccaacgacca caacgccgcg
     3001 ggcgcgcgcg agatcgccga tcacctggtg gcgagtttcg cgtcgatgct ctgctactgg
     3061 tttcacttct cgcacagcag ccggcgcatc cacgtcgaga ccgacgacga ttccgtgggc
     3121 ggtcacttcc ttcacctgct gcatcgccgt cagccgagcc cgagctgggt gcgcgccatg
     3181 cacacctcgc tcaacctcta cgccgagcac gaattcaatt catccacgtt cacggcacgc
     3241 gtgatcgcgg gcacgggatc cgacatctac tcgtgcatca ccggcgccat cggcgcgttg
     3301 cgtggtccca agcacggcgg cgccaacgaa gtcgcgctcg agatccagga gcgttacgcc
     3361 acacccgacg aagccgaggc cgacatccgc cggcgcgttg ccgcgaagga gatcgtcatc
     3421 ggcttcggac atccggtcta caccgtgtcc gacccgcgca atgtcatcat caagcgggtg
     3481 gcggagaagt tagggcgcga agccggcgac atgaagatgt acaacgtcgc cgaacgcatc
     3541 gaaggtctca tgggcgacat caagaagatg ttcccgaatc tcgactggta cagcgccgtg
     3601 agctaccacc tcatgggcgt gcccacggcc atgttcacgc cgttgttcgt cctgtcgcgt
     3661 gtcaccggct ggagcgcgca cgtcatcgag cagcgcgaag acggcaagat catccgcccg
     3721 gccgccaact acatcggccc ggaaaaccgc aagttcgtgc cactggccga acgtccctga
     3781 acaagaacat ggtgtcgtag atgtcatctc acgattacaa gtccgcgcag cggccgaacc
     3841 cggacgagct cctggtcgcc atcgccgact acacgaagaa ctaccaggta gtcagcgcac
     3901 aggcgtacga gacggcacgc tactgcctca tggacacgct ggcctgcggg ttccaggcgc
     3961 tcaaatatcc ggcgtgcacg cggttgttag ggcccgtcgt gccaggcgcc acgatgcccg
     4021 gcggctcgcg cgtgccgggc acgagttacg aactcgaccc cgtgatgggc gccttcagca
     4081 tcggcgccat ggtgcgctgg ctcgacttca acgacacctg gctggcggcg gagtggggtc
     4141 acccttccga caacctgggc gccatactcg cggtgtccga ctatctatcg cgccgcgcca
     4201 tcctgctcgg cgagaagccg ctgacggtgc gcgacgtgct caccgccatg atcaaggcac
     4261 atgaaatcca gggcgtgctc gcgctcgaga acagcttcaa tcgcgtgggc ctcgaccacg
     4321 tgctgctggt gcgcgtcgcc tcgacggccg ttgccacggc gatgctcggt ggatcgctcg
     4381 agcagatcgt gaatgcgctg tcgaacgcgt ggatagacgg cggcgcgttg cgcacgtacc
     4441 gccacgcgcc caacaccggc tcacgcaaga gctgggcggc aggcgacgcc acgagccgcg
     4501 cggttcgcca cgcgcttttc gccctgaaga acgagatggg atatccatcg gcgctgagtg
     4561 cgaagacctg gggcttccag gatgtcctgt tcaagggaaa ggcgctcacg ctcgcgcaac
     4621 ctctgggcag ctacgtcatg gagaacgtgt tgttcaagat cagcttcccg gcggagttcc
     4681 acgcgcagac agcggtggaa tgcgcgatgg cgctgcatcc gaaggtgaag gacaaactcg
     4741 ctctcatcga ccgcatcgtc atcgaaacgc aggaacccgg tgtgcgcatc atcgacaaga
     4801 ccggaccact ctcgaatccg gcggatcgcg accactgcat tcaatacatg gtcgcggtgc
     4861 cgctgatctt cggccgtctc accgcggcgg actatgaaga ctccatcgcc aacgatccgc
     4921 gcatcgacct gctccgcagc aagatggaag tacgcgagaa caccacgttt acgaaggaat
     4981 actacgagcg cgacaagcgc cacatcggca acgcgctgca catcttcttc agcgacggca
     5041 gtcccaaact ggagctgcgc atcgacttcc ccgtggggca tcgcaagcga cgcgccgagg
     5101 gcatgccact gctggtgcag aagttcgaat cgtcgg
//
LOCUS       DATDEU010000415         4173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1101334, whole genome shotgun sequence.
ACCESSION   DATDEU010000415 DATDEU010000000
VERSION     DATDEU010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101334"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1946)
                     /locus_tag="VIV63_09915"
     CDS             complement(66..1946)
                     /locus_tag="VIV63_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HEY6124959.1"
                     /translation="MKDSFLENTLHIFAAARASDDGQRCAVSMSPEAREKLKAALASA
                     RGQGGFPEALSMRASEPRRLGFNDGVIIPPDLFPIGTSPSRIAAAAADRAPLRGVVRI
                     IVVLVDFTDKHMAQTAQHFRDLFFGQGGAKPSVRDYYAEVTNGLIDIQGDVVGPFRMP
                     QTLAAYAHGASGIGNALPNAQTMASDALTAANPTVNFTPFDNDGNGFVDAFIVVHAGT
                     GAETNSNPGDIWSHKWTLSGGARNVDSTKVFAYLTVPEDCRIGVCAHELGHLLFGFPD
                     LYDTDNSSEGIGNWCLMAGGSWGAGGDAPTHPCAWCKANQEWVQVDNRTANGVVQIPD
                     VKSNHTVHRLWKDGAASKEYFLVENRQKTGAFDVSLPGSGLLIWHIDENMTNNRDENH
                     YWVALMQADGLRQLEDNTNRGDPGDCWPGSTNKTNFTNASTPNSKSYAGQQTCVAVTG
                     ISPSAAIMSANLQVKCGIKPSKEFTKEKELAKELRKEVLKEKEIRKDFVKEKELRKEV
                     AKDFIDEKSLRLEKRPDKTLVEGKLGDGKLTEGRPGGFGFDFGRSGAGEGDRLEELRD
                     RVSAIEAALSGAAAGGAPFIGSELRPDLSQGALSAEALYPDDQSGARMAPSKRLLDSG
                     PG"
     gene            complement(2238..3611)
                     /locus_tag="VIV63_09920"
     CDS             complement(2238..3611)
                     /locus_tag="VIV63_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124960.1"
                     /translation="MKELKMAALAALAFACVQSAGAQTPTQACKYATRMASGQDVAVV
                     VASDGSIWYASQSANRIVRIDPKGGETPFVPVDGSTKGLSGMLLDGKGNVWFSKRGGN
                     VGVIGRFPEAGGEGVEFAIPEQHAFPDGLVMGPDGMIWYFDPVKHKIGRVSTDGSMEI
                     FEGPPGLNPGITPTDMAAGTDKSLWITDLGENSLFRFDLATKGWKRYDIPEPQAHPKH
                     VRVAKDGTVWFTMVARNKLGRLKNGAFTSIDIQNENPNGIHVAQDQSLWFTTTNSTLG
                     RIKPDGTREKYRCNNVVGPIATASDGTVYALGNPNLLILKPGDTIAPPKVVSASNVQG
                     PSMMPLGPSPVKEVTLPELRKLFDDKTKNLVVHYNVLPKRGCGPCDESVAVFDEFARK
                     NAGKATFVRIANEYSEAAWSDPWYRANAALTGVPTFITYHDHKEVARVTGRESVPVLE
                     SKLLPVK"
     gene            3921..>4173
                     /locus_tag="VIV63_09925"
     CDS             3921..>4173
                     /locus_tag="VIV63_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124961.1"
                     /translation="MIAIGLASIVYGNDSFLWYTIPDMPGSATLVYLCGLVALASGIV
                     LFLPTYLLRACRTLVVYLGLWLVVLKVTTLLGSPGEMMRW"
BASE COUNT          705 a         1316 c         1278 g          874 t
ORIGIN      
        1 gccgtcgagt cgaaagcatg cgcaagaatc agcacgcgca cggtgcagtc tccctctcgg
       61 cctgatcaac ctggacctga atcgagcagg cgcttgctgg gcgccatccg cgcgccagat
      121 tggtcgtctg gatacaacgc ctcggcagat agcgcgccct gactgagatc cgggcgcaac
      181 tcgctaccga tgaagggcgc gccgcccgct gccgcgccgc tcaatgccgc ttcgattgcc
      241 gagacgcgat cgcgcagttc ttcgagccga tcgccttcgc cagctccgct acgcccaaag
      301 tcaaagccga atccgcccgg ccggccttcc gtgagcttgc cgtcaccgag cttgccctcc
      361 accagggttt tgtccggccg cttctcgagg cgcagcgact tctcgtcgat gaagtccttc
      421 gccacctcct tgcgcagctc cttctccttg acgaagtcct tgcggatctc tttttccttg
      481 aggacttcct tgcgcaactc cttggcgagc tccttctcct tggtgaactc cttgctcggc
      541 ttgatgccgc atttcacctg caggttcgcg ctcatgatcg ccgcggaggg agagatgccc
      601 gtgacggcga cacaggtctg ctggcctgcg taggatttcg agttgggcgt ggaagcgttc
      661 gtgaagtttg tcttgttagt cgagcccggc cagcagtcac cgggatcgcc acgattggtg
      721 ttgtcctcga gctgtcgcag cccatccgcc tgcatcaacg caacccagta gtggttctcg
      781 tcgcggttgt tggtcatgtt ctcgtcgatg tgccagatga gaaggccgga gcccggcaac
      841 gaaacgtcga aagcaccagt cttctggcgg ttctcgacga gaaagtattc cttgctggcg
      901 gcaccgtctt tccacagccg atggacggtg tggttcgact tcacgtctgg tatctgcacg
      961 acgccattgg cggtgcgatt gtcgacttgc acccattcct ggttcgcctt gcaccaggcg
     1021 caaggatgcg tcggcgcgtc gccgccggcg ccccaactac cacctgccat gaggcaccag
     1081 ttgccgatgc cttccgagga gttgtcggtg tcgtagaggt cggggaatcc gaacaacaga
     1141 tggccgagct catgcgcgca aacgccgatg cggcaatctt ccggcacggt gaggtaggcg
     1201 aacactttcg tcgaatcgac attgcgtgcg ccgccgctga gcgtccattt gtgcgaccag
     1261 atatcgcccg gattcgagtt ggtttcggcg ccggtgccgg catgcacaac gatgaaggcg
     1321 tcgacgaatc cattgccgtc gttgtcaaag ggcgtgaagt tcacggtcgg attcgcagcg
     1381 gtcagcgcgt cgcttgccat cgtctgggca ttcggcagag cgttaccgat gcccgaggcg
     1441 ccatgcgcgt aggccgcgag cgtctgcggc atgcggaagg ggcccacgac atctccctgg
     1501 atgtcgatca gtccgttcgt cacctctgcg tagtagtcgc gcacgctggg ttttgcgccg
     1561 ccctgcccga agaacaggtc acggaagtgc tgcgcggtct gtgccatgtg cttgtcggtg
     1621 aaatcgacga gcacgacgat gatgcgcacc actccgcgga gcggcgcgcg gtccgccgcg
     1681 gctgctgcga tgcggctggg agatgtgccg atagggaaca ggtccggcgg aatgatcacg
     1741 ccgtcgttga aacccaggcg ccgtggctcg gaagcgcgca tcgacagcgc ttccggaaag
     1801 ccgccctggc cacgcgccga tgcgagcgct gctttcagtt tttcccgtgc ttccggggac
     1861 atggacacgg cacagcgctg tccgtcatca ctggcgcggg cggccgcgaa gatgtgcaac
     1921 gtgttctcga gaaacgagtc tttcattgtc gtagctccgt gtatgcagga gttgttgttg
     1981 ctcgaatgcg gcggaccgtc agcgatcgac acgcggctgt cgtctacaaa gagaaatctg
     2041 tcgcgacctg gccgccgatt tcacgagcag acataaatct tcgtttcggt ggtggcatgc
     2101 accgcatcgc gccatgcgcg gacgggaagc ggcaagtcca gaaacagaaa gcccccggca
     2161 gtttcccgcc gggggctttc taacttctct ggcagcctga catccccagg ctgatcgccc
     2221 ttagggcact ctgacgatca cttcaccggc agcaacttcg actcgaggac cggcacggac
     2281 tcgcgtcccg tcacgcgcgc gacttccttg tggtcgtggt aagtgatgaa ggtaggcacg
     2341 ccggtcaggg cggcattggc cctgtaccac ggatcgctcc atgcggcttc gctgtactcg
     2401 ttcgcgattc gcacgaacgt ggccttgccg gcattcttgc gcgcgaactc atcgaatacc
     2461 gcgaccgact cgtcgcaggg tccacatccc ctcttcggca gcacgttgta gtgcacgaca
     2521 aggttcttcg tcttgtcgtc gaacagcttg cgcagttcgg gcagcgtcac ttccttcacg
     2581 ggactcggcc ccagcggcat catgctcggg ccctgcacgt tgcttgcgct cacgaccttg
     2641 ggcggggcga tcgtgtctcc gggttttaga atgagtagat tgggattgcc cagcgcgtac
     2701 accgtgccgt ccgacgcggt ggcgatggga cccacgacgt tgttgcatcg atatttctca
     2761 cgcgtgccat cgggcttgat acggcccagc gtgctgttgg tcgtggtgaa ccagaggctc
     2821 tggtcctggg caacgtggat gccattggga ttctcgttct ggatgtcgat cgaggtgaac
     2881 gcaccattct tgaggcgacc cagcttgttt ctggccacca tcgtgaacca cacggtgccg
     2941 tctttcgcga cgcgcacatg cttgggatga gcctggggct cgggaatgtc gtagcgcttc
     3001 catcctttcg tggcgagatc gaacctgaac agcgagttct cgccgaggtc ggtgatccac
     3061 aatgatttgt cggtgccagc ggccatgtcc gtgggcgtga tgcccggatt gagcccgggt
     3121 ggtccctcga agatttccat actgccgtcc gtgctgacgc ggccgatctt gtgtttcacc
     3181 ggatcgaagt accagatcat tccatcgggc cccatgacga gcccgtccgg aaacgcgtgc
     3241 tgctccggga tggcgaactc cacgccttcg ccgccagcct cgggaaatct gccgatcacg
     3301 ccgacgttgc cgccgcgctt cgagaaccag acattgccct tgccgtcgag caacatgccg
     3361 gagagccctt tggtggaccc atctaccggc acgaacgggg tctcgccacc cttcgggtct
     3421 atccgcacga tgcgattggc gctttgcgag gcgtaccaga tgctgccatc gctggcgacc
     3481 acgacggcca cgtcttgtcc gctcgccatg cgtgtcgcgt acttgcaggc ttgcgtcggc
     3541 gtttgggcgc cggcggattg cacgcaggcg aatgccaggg ccgccagtgc ggccatcttg
     3601 agttctttca tcgtcgactc cctgatctgt gttcagcgaa tgcagaggtc tctgccgcgt
     3661 cccggaattg cgtgacaacg ccagggccgg aagcaccagc agccgcgccc aagaggaaaa
     3721 cctggttcga caccacggcc gcgtcttcgc cagcgttctc gacgatctga cacagcggct
     3781 cttctaacaa aagcaaaacc cgcggcagcg ataagccagt cgcgccgcgc aaagtcggga
     3841 atgccctata gtggcgcttc gcacgtcaga cggaggcagc aatgaccact caacccagtc
     3901 gcgcagcttt ggcgatcgcc atgattgcca tcggcctcgc cagcatcgtc tacgggaatg
     3961 actcgttcct ctggtacacg atcccggaca tgccgggcag tgcaacgctg gtttatctct
     4021 gcggcctggt cgcgctcgcc tcgggaatcg tcctgttcct tccgacctat ctactgcgtg
     4081 cgtgccgcac actggttgtg tacctcggcc tgtggttggt cgtgctcaag gtgacgaccc
     4141 tgctgggatc acccggcgag atgatgcggt ggg
//
LOCUS       DATDEU010000416         3718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_662998, whole genome shotgun sequence.
ACCESSION   DATDEU010000416 DATDEU010000000
VERSION     DATDEU010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662998"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..698)
                     /locus_tag="VIV63_09930"
     CDS             complement(9..698)
                     /locus_tag="VIV63_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6124962.1"
                     /translation="MSTRPTIYSGPLSMFGAKVQIAALEKGLDFDLVMVPYDSARGYD
                     PRHPEVLRINPKRQVPVLVHGPVEIFDSTQIFEFLEDMAPVPPLWPRDVIARAEARNL
                     EHRSDEVYFPHIIRLMSLQDALSTEPALTAIAAAQRFHEDMDQRLATREWLAGSFSFA
                     DIAFYMAALFGERQSAPLTASTPRLLAWRERMGQRAPVVTVVGAMATWLRNAGRPVPP
                     FMAEIQARKDR"
     gene            complement(829..1431)
                     /locus_tag="VIV63_09935"
     CDS             complement(829..1431)
                     /locus_tag="VIV63_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY6124963.1"
                     /translation="MSFATPKQLETWMRKNHASAPELWLKVHKKDSGLRSVTCAEALD
                     VMLCWGWIDGLRRGFDEQSFLQRYSPRRAKSIWSQINRDHVARLTAAGRMTPHGQKQV
                     DAAKADGRWGAAYAPIRSVSRDTMPADLLAAIKANAKAQKKFETLGRMNLFALGFRTN
                     NMKTAAGREKKIATLVAMLARGETIVPEKAKKPKAPPKRR"
     gene            complement(1441..2205)
                     /locus_tag="VIV63_09940"
     CDS             complement(1441..2205)
                     /locus_tag="VIV63_09940"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6124964.1"
                     /translation="MNIRALIVDDEPWARTRIASLLKAEADFEIVHECADGAEAVRAI
                     VGLQPQVVFLDVQMPGIDGFDVVDAIGAAAMPVVVFATAHQEFAVRAFDAGAVDYLLK
                     PFDEERFRRAVERVRRDLRAPEAATAALENAMTQVRKGVPYAERLVVNNAGRVLFVKV
                     ADIDWFEAAGNYVTIHAGREKHMLRETLRGLSERLDPKVFVRLHRSSVVNVERLRELL
                     PWSRGEQVAVLKDGTHLPIGRAFREHLMTVMNGAGQ"
     gene            complement(2132..3226)
                     /locus_tag="VIV63_09945"
     CDS             complement(2132..3226)
                     /locus_tag="VIV63_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6124965.1"
                     /translation="MESLLRGRWTRWVLFLLGWAALSLLFAPEAYLAFYLRNAPISWL
                     ETVELTVINSAIALVFIPGIVFLTRRFPIERGRWQRSLGVHVAACLAFSVAHSFLYAV
                     ACHAWRDVGETLFVRFHPNLITYWAFVGFTQAFEYFRKYQQREREITRLQLQALKAQL
                     QPHFLFNALNTVAAMMHVDVKRADRMLSRLSELLRMTLNNIERQEVRLSDEIAFVEAY
                     LDIERERFGGRLAMRIDADPDTLDALVPALFLQPLVENCVRHGFTTPDAESLIEIRAV
                     RDGERLTLTVTDNGRGFVATPREGVGITNARKRLAQLYDRQSLSIAARTRGVVVTVEL
                     PFHKEPDEHPSADRGRRALGAHADRLAAEG"
     gene            complement(3240..>3718)
                     /locus_tag="VIV63_09950"
     CDS             complement(3240..>3718)
                     /locus_tag="VIV63_09950"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6124966.1"
                     /translation="GQGRGIAVANWGNWGGKDQVGGTTAAVVAHVDVSHEGVLKVLQL
                     DIAFDCGRVFNRDAVEAQMQGGLLFGLNMALNEELNIENGRIVEGNFDTYPMLRLADA
                     PKLRVHFGGLTGAERSAEVGEPPVGPVGPAVANAIFAATGKRIRSMPFRKHDLRWA"
BASE COUNT          637 a         1210 c         1203 g          668 t
ORIGIN      
        1 ctggccagtt agcggtcttt tctcgcctgg atttccgcca tgaatggcgg caccggccgc
       61 ccggcattgc gcaaccacgt tgccattgcg cccaccacgg tcacaaccgg cgcgcgctgt
      121 cccatgcgct cgcgccacgc gagcaggcgc ggtgtgctcg cggtcaacgg cgcgctctgt
      181 cgctcaccga acaatgcggc catgtagaac gcgatgtccg cgaacgaaaa acttcccgcg
      241 agccattcgc gggtagccag gcgctggtcc atgtcttcgt gaaagcgctg cgcggccgcg
      301 atggcggtca gcgcgggttc cgtgctcagc gcatcctgca ggctcatgag ccggatgatg
      361 tgcgggaaat acacttcgtc ggatcgatgc tcgagattgc gcgcctccgc gcgcgcgatg
      421 acatcgcgcg gccacagcgg cggaacgggc gccatgtctt cgagaaactc gaagatctgc
      481 gtggaatcga agatctccac gggcccgtgc accagcaccg gcacctgtct cttgggattg
      541 atgcgcagga cctcgggatg ccgggggtca taacccctcg ccgagtcgta cggaaccatg
      601 acgaggtcga agtcgagccc cttctccagc gccgcgatct gcaccttggc gccgaacatg
      661 ctgagcggcc cggagtagat ggtggggcgc gtgctcattt ctttttcccg ggcgcgctcg
      721 tttcgcaggt gtggaacgtg atgaagtcgg atgactcgag ctggatcttc tcgatggcgt
      781 tgagcttcga cacggaattc tcccccgggg attccgtgcg agcctacatc atctccgctt
      841 gggcggcgcc ttcggtttct tcgccttctc cggcacgatg gtttcgccgc gtgccagcat
      901 cgccaccagc gtggcgatct tcttctcgcg ccccgcggcg gtcttcatgt tgttagtgcg
      961 gaacccgagc gcaaacagat tcatgcgccc cagcgtctcg aatttcttct gcgccttcgc
     1021 attggccttg atggcggcga gcagatccgc gggcatggtg tcgcgagaaa cgctgcgaat
     1081 gggcgcatag gcggcgcccc atcggccatc ggctttcgcc gcgtcgacct gcttctgacc
     1141 gtgcggcgtc atgcgaccgg ctgccgtcag gcgcgcgaca tgatcgcgat tgatctggct
     1201 ccagatgctc ttcgcgcggc gcggtgaata acgctgcagg aaggactgct cgtcgaaacc
     1261 ccggcgcagc ccgtcgatcc agccccagca caacatgacg tccagcgcct ccgcacaggt
     1321 caccgatcgc agtccggagt ccttcttgtg caccttgagc cacaactccg gcgcgctggc
     1381 gtggttcttg cgcatccagg tttcaagctg cttcggggtt gcgaaagaca tcgactccat
     1441 ctactgccct gctccattca tgaccgtcat cagatgttcg cggaatgcgc ggccgatggg
     1501 aaggtgcgtt ccatccttga gcacggccac ctgctcgccg cgcgaccagg gcaggagttc
     1561 acgcagacgc tccacgttga ccaccgatga ccggtgcagc cgcacgaaga ctttcggatc
     1621 gagacgttcg gagaggcctc gaagcgtctc gcgcaacatg tgcttctcgc gacccgcgtg
     1681 gatggtgacg taattgccgg cggcctcgaa ccagtcgatg tcggccacct tgacgaacaa
     1741 cacgcggccc gcgttgttca cgacgaggcg ttcggcatat ggcacgccct tgcgcacctg
     1801 cgtcatcgca ttctcgagcg cggccgtggc cgcttcgggc gcgcgcagat cgcgccgcac
     1861 ccgttcgacg gcgcggcgaa agcgctcttc gtcgaaaggc ttgagcagat agtcgacggc
     1921 gccggcgtcg aacgcacgca cggcgaattc ctggtgcgcg gtcgcaaaga cgacgacggg
     1981 catggccgcc gcgccgatcg catcgaccac atcgaagccg tcgatacccg gcatctgcac
     2041 gtcgaggaac accacctgcg gctgcaggcc gacgatggcg cgcacggctt cggcgccgtc
     2101 agcgcactcg tgcacgatct cgaaatccgc ctcagccttc agcagcgagg cgatccgcgt
     2161 gcgcgcccag ggctcgtcgt ccacgatcag cgctcggatg ttcatcaggc tccttgtgga
     2221 atggcagctc gaccgtcacc acgacaccgc gggtacgcgc ggcgatgctc aggctttgcc
     2281 ggtcgtagag ttgcgcgaga cgcttgcggg cattcgtgat accgacgcct tcgcgcggcg
     2341 tcgccacgaa accgcggcca ttgtcggtca cggtgagcgt gaggcgttcg ccgtcgcgca
     2401 ccgcgcgaat ctcgatgaga ctctcggcat cgggcgtggt gaagccgtgc cgcacgcagt
     2461 tctcgacgag cggctgcagg aacaacgccg gcaccagcgc atcgagcgtg tcgggatcgg
     2521 catcgatgcg catggcgaga cggccgccga atcgctcgcg ctcgatgtcc aggtaggcct
     2581 cgacgaaggc gatctcgtcg gaaaggcgca cttcctgacg ctcgatgttg ttgagcgtca
     2641 tccgaagtag ttcggatagg cggctcagca tgcggtcggc acgtttcaca tcgacgtgca
     2701 tcatggccgc cacggtgttc agtgcgttga acaggaagtg aggctgcaac tgcgccttga
     2761 gcgcctgcag ctgcagccgc gtgatctcgc gctcgcgctg ctggtacttg cggaaatatt
     2821 cgaacgcctg cgtgaatccg acgaacgccc agtaggtgat caggttggga tggaagcgga
     2881 cgaacagtgt ttcgccgacg tcacgccacg catggcaggc cacggcgtat agaaaggagt
     2941 gcgcgacgga gaacgcgaga cacgccgcga cgtggacgcc gagtgagcgc tgccatcggc
     3001 cgcgttcgat cggaaaccgt cgcgtgagga agacgatgcc cgggatgaac acgagcgcga
     3061 tcgcggagtt gatgacggtg agctcgaccg tctccagcca cgagatcggc gcgttgcgca
     3121 gatagaacgc gagatacgcc tcgggcgcga acagcaacga cagcgcggcc catcccagga
     3181 ggaacagcac ccatcgggtc catcgtccgc gtaacagcga ttccatgctg cggacgttat
     3241 caggcccagc gcaggtcgtg cttgcggaac ggcatggagc ggatgcgttt gcccgtggcg
     3301 gcgaagatgg cgttcgccac ggcgggtccc accggcccca ccggcggctc gcctacttcg
     3361 gcgctgcgct cggcgcccgt gaggccgccg aaatgcacgc gaagtttcgg ggcatcggcc
     3421 aggcgcagca tgggataggt gtcgaagttg ccttcgacga tgcgcccgtt ctcgatgttg
     3481 agctcttcgt tgagcgccat gttcaggccg aacagcaggc cgccctgcat ctgtgcttcg
     3541 accgcatcgc ggttgaacac gcgtccgcag tcgaaggcga tgtcgagttg caggaccttg
     3601 agcactcctt cgtgcgagac gtcgacgtgc gcgaccacgg cggcggtcgt gccacccacc
     3661 tggtccttgc cgccccagtt gccccagttg gcgaccgcga tgccgcggcc ctggcctt
//
LOCUS       DATDEU010000417         3993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_369352, whole genome shotgun sequence.
ACCESSION   DATDEU010000417 DATDEU010000000
VERSION     DATDEU010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369352"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VIV63_09955"
     CDS             <1..1458
                     /locus_tag="VIV63_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6124967.1"
                     /translation="AFAAAGDDERAQALIWRALELEPNEETALNWLIGMVSAKGQEAV
                     LAAYTRASGLRGSWRALLWLARYALERGDLAEATRLYEEVLTRANPAPPDLLMQMSGD
                     LGNRGHTELLIRLTQPRFDLAEHGLTVGNNLLRAYAELGMFAEARKLLEQLYSQQRPD
                     WREQLIAWEQKLDDAQKRYGEVTAPVEVVVMKLEQPVWARGVLGFDAVLPAKTQAAPR
                     IHFICGSGEADDQTGGKVVSQPTNDLGRIARSVPMFLAEELFLRTNARTAFLLPWMKQ
                     GGFILSAKPWTRAFLPPDHAPPDLIVYLHIDARVSPWLMKVTIENAQRDATPVVFEQA
                     FTLQTAGRDIQSLLYDLIPRLTILLALRREESNSALGTPPPELLPGYLAAIEQALAVG
                     LAARQPGGESFLHQERAIFDHLFDVALQSDQLLRPRMLLVNALENQARRRPDIVREYL
                     DKLELLQQRHVLAEGVGNQLVAKGVAIVGEKAKAS"
     gene            1473..2162
                     /locus_tag="VIV63_09960"
     CDS             1473..2162
                     /locus_tag="VIV63_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY6124968.1"
                     /translation="MNRLHEAIRRLPAGVEFLVVVTWAFGLPIFSSIMALGTPGSSEV
                     PVFTNAQLLGTIIFEVVQLGFLSWFLHIRGWTLEKFGLSFSWSGTLMGVALLVVTYGL
                     IAGVQQLAQGVFGYDLQAAIARAPRVDKELSMQLVFLVSVVNGIFEELFVAGYIITAL
                     TARRGMWVAINVSVVVRLLYHLYQGPIGVLTIVPMGLLYGYLYSRTRQLWPLVVAHVL
                     LDIIALSLRLA"
     gene            complement(2235..3086)
                     /locus_tag="VIV63_09965"
     CDS             complement(2235..3086)
                     /locus_tag="VIV63_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6124969.1"
                     /translation="MGTNNIVLVHGLWMTPLSWEYWAHHYTERGYSVYAPSWPGMERD
                     VRALRRAPETYANLGFRQIVDHYEKLVIDLDSAPIIIGHSFGGLVVQALLDRGLGACG
                     VGLASAPIKGIWTLPYSTMRVVTPQLINPRNIRRCVPLTPAQFHYAFMNTASAEESFR
                     IYQRYAVPGPDHILFQTELANFNPFAETQVNVRRNNRAPLLMIAGSRDHLIPPRTVKA
                     NVKAYRHSTAVTEYKEFPDRTHFIIGQSGWSEVANYTLDWARDQQLLIDRERRRVARE
                     LQARQVA"
     gene            3223..3525
                     /locus_tag="VIV63_09970"
     CDS             3223..3525
                     /locus_tag="VIV63_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6124970.1"
                     /translation="MEEPTRQEIDAAPGATVLEFGASWCGYCQAAQENITAVLKRHPA
                     VRHIKIEDGKGKPLGRSFKVKLWPTLVYIENGVEKGRVVRPGNIADIEAIFGGAAA"
     gene            3522..>3993
                     /locus_tag="VIV63_09975"
     CDS             3522..>3993
                     /locus_tag="VIV63_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124971.1"
                     /translation="MRLADFRRGLAAALTISLLLAAAPSGANDADESAYTPSTLAKIM
                     GGAARSQNDEAELAPPGQWFTTTATFTGQKRQPSSRFRPLISSWLSSRGMDGGLVDMY
                     FGGELPELQFEQAGKKYWVMAGLPGGLGFDVYPPGQKLAIYLQRVGFSSGTPVAV"
BASE COUNT          698 a         1248 c         1306 g          741 t
ORIGIN      
        1 gcctttgcgg ccgccggtga cgacgaacgc gcgcaggcgc tcatctggcg cgcgctcgaa
       61 ctcgaaccca acgaggagac cgcgctcaac tggctgatcg gcatggtcag cgcgaaaggt
      121 caggaggccg tgctggccgc ctatacgcgg gcttcgggcc tgcgcggcag ctggcgcgcg
      181 ttgctgtggc tcgcgcgtta tgcgctcgag cgtggcgatc tcgccgaagc gacgcggctg
      241 tacgaagagg tgctgacccg cgccaacccg gcgccgccgg atctgctgat gcagatgagc
      301 ggcgacctcg gcaatcgggg ccataccgaa ctgctcatca ggctgacgca gccgcgcttc
      361 gatctcgccg agcatgggtt gaccgtgggc aacaacctgc tgcgcgcgta cgcggagctc
      421 ggcatgttcg ccgaggcgcg aaagttgctc gaacaactct attcgcagca gcggccggac
      481 tggcgcgagc agctcatcgc ctgggagcag aagctcgacg atgcgcagaa gcgttacggc
      541 gaagtgacgg cgcccgtgga agtggtcgtc atgaagctcg aacaaccggt gtgggcgcga
      601 ggcgtcctcg ggttcgatgc ggtgttgccg gccaagacac aggctgcgcc gcgcatacac
      661 ttcatctgcg gtagtggcga agccgacgac cagaccggcg gcaaggtcgt ctcgcagccc
      721 accaacgatc tggggcgcat cgcgcgatcg gtgcccatgt tcctcgccga ggagcttttt
      781 ctgcgcacca acgcacgcac cgcgtttctg ctgccgtgga tgaagcaggg tggtttcatc
      841 ctatcggcca agccgtggac tcgagcgttc ctgccgcccg accacgcgcc gccggatctc
      901 atcgtctatc tgcatatcga cgcgcgcgtg tcgccgtggc tgatgaaggt gaccatcgaa
      961 aatgcgcagc gcgacgccac gccggtggtg ttcgagcagg cgttcacgct gcagacggcc
     1021 ggccgggata tccagtcgct gctgtacgac ctgatcccgc gcctcaccat cctgctcgcg
     1081 ctgcggcgcg aggaatcgaa ctcggcgctc ggcacgccac cgcccgagct gctgcctggc
     1141 tatctggccg ccatcgagca ggccttggcc gtcgggctcg ccgcgcggca accggggggc
     1201 gaatcgtttc tgcatcagga acgcgcgatc ttcgatcacc tgttcgacgt ggcgctgcag
     1261 agcgatcagc tcctgcgtcc tcgcatgctg ctcgtgaacg cgctcgagaa tcaggcgcga
     1321 cgccggccgg acatcgtgcg ggaatacctg gacaagctcg agctgttgca acagcggcac
     1381 gtgctggccg aaggcgtggg taaccagctg gtggcgaaag gcgtagcgat cgtgggcgaa
     1441 aaagcgaagg caagttaagc ctcgagattg cattgaatcg actgcacgaa gccatccgcc
     1501 gcctgccggc cggcgtcgag ttcctggtcg tcgtcacgtg ggcgttcggc ctgcctatct
     1561 tctcgtcgat catggcgctc ggcacaccgg gcagcagcga agtcccggta ttcaccaacg
     1621 cgcagctgct gggaaccatc atcttcgagg tggtccagct cggtttcctg agctggttcc
     1681 tgcacattcg cggctggacg ctcgagaaat tcgggctgtc gttttcgtgg agcggcacgc
     1741 tgatgggcgt ggcgctgctc gtcgtcacgt atgggttgat cgcgggggtg caacagctcg
     1801 cgcagggagt gttcggctac gacctccagg cggccatcgc gcgcgctccg cgggtggaca
     1861 aggaattgag catgcagctc gtgttcctcg tgtccgtggt caacgggatt ttcgaagagt
     1921 tgtttgtcgc cgggtacatc atcaccgcgc tgaccgcgcg gcggggcatg tgggtggcca
     1981 tcaatgtgag cgtggtggtg cggctgctgt accacctgta ccagggtccg atcggcgtgc
     2041 tgaccatcgt cccgatggga ctgctgtacg gctacctgta ttcgcgcaca cgccagcttt
     2101 ggccgctggt ggtggcgcac gtgctgctcg acatcattgc gctgtcactg cgccttgcgt
     2161 gaaaaaaagg ggacagatct attttccgca acggaaaata gatctgtccc ctttttttcc
     2221 gtcccctttt ttcttcacgc gacctgccgc gcctgcagct cccgcgcgac tcgccgccgc
     2281 tcgcggtcga tgagcagctg ctggtcgcgc gcccaatcga gcgtgtagtt cgccacctcc
     2341 gaccatccgc tctggccgat gatgaagtgc gtgcgatccg gaaattcctt gtattcggtg
     2401 accgcggtgg aatgccggta ggccttcaca ttggccttga cggtgcgcgg cgggatgagg
     2461 tggtcacgcg agcccgcgat catgagcaac ggcgcgcgat tgttgcgccg gacgttgacc
     2521 tgcgtttccg cgaacggatt gaaattcgcg agctcggtct ggaacaggat gtgatccgga
     2581 ccgggcaccg cgtagcgctg gtagatgcga aacgattcct ccgccgaggc cgtattcatg
     2641 aacgcgtagt ggaactgcgc cggcgtcagg ggaacgcagc ggcgaatgtt gcggggattg
     2701 atcaactgcg gcgtgaccac gcgcatcgtg gaatagggca gtgtccagat acccttgatg
     2761 ggcgccgacg ccaggcctac gccgcaggct cccagccccc ggtctaacaa tgcctggacc
     2821 acgagtccgc caaaagaatg cccgatgatg atgggcgcgc tgtcgagatc gatgacgagc
     2881 ttctcgtagt ggtccacgat ctgcctgaaa cccaggtttg catacgtttc cggggcgcga
     2941 cgcaaggccc gcacgtcacg ttccatgccg ggccagctcg gcgcatacac gctgtagcca
     3001 cgttccgtgt agtggtgcgc ccaatactcc caactgagcg gcgtcatcca taacccgtgg
     3061 accaggacaa tgttgttagt gcccatgagg cctccaccag tgtcaggaga ttcaccctcg
     3121 tggttgtcgt tgtccagcga cagggggctg catttgtacg tagtagaaac cggaagaaag
     3181 ctctaacaac acacgcctcg gattttcacc taggattgcg caatggaaga accgacgcgt
     3241 caggaaatcg acgctgcccc cggcgcaacc gtcctcgagt tcggcgccag ctggtgcggc
     3301 tactgccagg cggcacaaga gaacatcacc gcggtgctga aacgccatcc cgcggtgcgc
     3361 cacatcaaga tcgaagacgg caagggcaag ccgctgggac gctcgttcaa ggtgaagttg
     3421 tggcccacgc tcgtctacat cgaaaacggt gtggaaaagg gccgggtcgt gcggccgggc
     3481 aatatcgccg atatcgaggc gatcttcggc ggggccgctg cttgaggctt gcggacttcc
     3541 gccgcggtct tgccgccgcg ctcaccattt ccctcctgct ggccgccgca ccgtccggcg
     3601 ccaacgacgc cgatgaatcg gcgtacacgc cgtcaacgct cgccaagatc atgggtggtg
     3661 cggcgcgttc gcagaacgat gaagcggaac tcgcgcctcc cggtcagtgg ttcaccacca
     3721 cggcgacgtt caccgggcag aagcgccagc cgtcgagccg cttccggccg ttgatcagtt
     3781 cctggctgag cagccgcggc atggatggcg gcctcgtgga tatgtacttc ggcggcgagc
     3841 tgcctgaact gcagttcgag caggccggca agaaatactg ggtcatggcg ggcctgcccg
     3901 gcggactcgg attcgacgtg tatccgcccg gccagaagct ggctatctac ctgcagcgcg
     3961 tgggtttctc ttcaggaacc cccgtggccg tgc
//
LOCUS       DATDEU010000418         4373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_517946, whole genome shotgun sequence.
ACCESSION   DATDEU010000418 DATDEU010000000
VERSION     DATDEU010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517946"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VIV63_09980"
     CDS             <1..400
                     /locus_tag="VIV63_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6124972.1"
                     /translation="QLEILQARPTDRGLVLTLGDTLFATGKSEIKSGATANLDRLTAF
                     LNEYPTRTASIEGYTDSMGSEEMNQSLSQRRADAVRGYLVGQGVGSTRLTSSGRGENS
                     PVADNGSAEGRQQNRRVEVVISQGNENGPK"
     gene            563..1783
                     /locus_tag="VIV63_09985"
     CDS             563..1783
                     /locus_tag="VIV63_09985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6124973.1"
                     /translation="MRNGIGDASTTLQNSIHCDIAIVGAGITAALVADALIATGKRIV
                     VLDSRDIAQGSTSASTALLQYEIDTHLVELVRTHGAEAAMRAYRACAASFTMLEQRFP
                     ELLPQAGYERRPSLYLASDEKAVETLRAEQAARRNIGLACEWLDADAVRSRFGCQRPG
                     GILSALGGLVDPFRLARGLFAASQRHGVQLYARTKVAQIETNPEALVLHTEGGNTVTA
                     AHVAVCAGYESLEFLPHDVADVNNTFALVTEPLRQRKQADTLPLVWESARPYLYLRGT
                     PDGRLMIGGADVPFKNAGARDLLLPRQVRRLQEQFQDLFGAEMPPVAYAWAGSFAETR
                     DGLPLIGRVPGMDPRLQFALCYGGNGITYSVHAGEMIRAGIEGRAHDLDDVFGFGRLG
                     TDLSAGRQRGIAGS"
     gene            2063..2512
                     /locus_tag="VIV63_09990"
     CDS             2063..2512
                     /locus_tag="VIV63_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124974.1"
                     /translation="MGFAPDTSGTRTYYSLLINAARAEVEPMSEPRNPAHVLGVGATI
                     ESVNGPVFAAYFASSRERFDAQPNYRSGLFVLVDGAVYAVKAEQIETSIRRGFSTRAF
                     TLLRGDRPQRSVVYPWPWYREPFVSGGSGVRSGDFLREMSSMVREYG"
     gene            complement(2523..3524)
                     /gene="gnd"
                     /locus_tag="VIV63_09995"
     CDS             complement(2523..3524)
                     /gene="gnd"
                     /locus_tag="VIV63_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY6124975.1"
                     /translation="MKEMKEVGFIGLGRMGANMVRRLTGAGFQCVVFDANPAAVRALY
                     GPGVTGTGSLKEFVETLKAPRVIWLMLPAAIVEQEINNLRPLLSAGDVLIDGGNSYYR
                     DDLRRGGELLKAGIHYLDVGTSGGIAGGENGYCLMIGGEKPVVDSLEPLFAALAPGGD
                     GPDSAARGFLHCGPYGAGHFVKMVHNGIEYGLMAAYAEGFNILRNANIGTRTHEKDAE
                     TTPLQHPEHYAYEFDLARIAEVWRHGSVVRSWLLDLTARALSEDAGLGAFAGRVSDSG
                     EGRWTVAAAVDEGVPAPVITAALYSRFESRGNADYGDRLLSAMRKQFGGHVEKKTGG"
     gene            3560..4258
                     /locus_tag="VIV63_10000"
     CDS             3560..4258
                     /locus_tag="VIV63_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY6124976.1"
                     /translation="MKVVRRSADRGHADHGWLKSYHSFSFAEYFDEDHVEFGPLRVLN
                     EDRVIGGQGFGTHGHRNMEIISYVLEGELTHQDSIGTRSTIRPGDVQRMSAGTGVRHS
                     EFNGSQTNKLHFLQIWIQPNAADIPPSYEEKHFDANDKRGRLRLIASSDGEGGSVLVH
                     QDVRVYSGLFDAGESARLELAPGRRAYIHVARGLVHANGVELSAGDALRLVDESVIEI
                     AQGSKSEVLVFDLP"
BASE COUNT          791 a         1379 c         1414 g          789 t
ORIGIN      
        1 ccaactcgaa atcctgcagg cgcggccgac ggatcgcggt ctggtgctga cgctgggcga
       61 cacgctgttc gccacgggca agtcggagat caagtccggc gcaacggcga acctcgaccg
      121 cctgacggcc ttcttgaacg agtatccgac gaggaccgcg tccatcgaag gttataccga
      181 cagcatgggc agcgaagaga tgaaccagtc actctcgcag cgccgcgcgg atgccgtcag
      241 gggttatctg gtcgggcagg gcgtgggttc gacccggctc acttcctcag gtcgcggcga
      301 aaattcgccg gtcgccgata acggttccgc cgagggacgt cagcagaatc gtcgcgtgga
      361 agtcgtgatc agtcagggca acgaaaacgg ccccaagtaa gcgattagag ccgaaaggca
      421 gcccggattg gcccgcataa cagcgggcca gtccgggctt tttcttacag ggcacgtatt
      481 cgccgccacc taagctggcc gcatgtggcc cttccgaaaa aacagcgcaa acccgatacg
      541 caccagcgag gcgtattggc tgatgcgcaa tggcatcggc gatgccagca ccactcttca
      601 gaattccatc cactgcgaca tcgccatcgt gggcgcgggc atcaccgctg cgctggtggc
      661 ggatgcactg atcgccacgg gcaaacgcat cgtggtgctc gattcgcgcg acattgcgca
      721 aggcagtaca tcggcgtcga ccgcgctgct tcaatacgag atcgataccc acctcgtcga
      781 actcgtgcgg acccacggcg ctgaagcggc gatgcgggcc tatcgcgcct gcgcggcgag
      841 tttcacgatg ctcgagcaac gatttcccga gctgttgccc caggccggtt atgaacggcg
      901 ccccagcctc tatctcgcgt cggacgaaaa ggccgtcgag acgctgcgcg ccgaacaggc
      961 ggccaggcga aacatcgggc ttgcgtgtga gtggttagat gccgatgcgg ttcggagtcg
     1021 cttcggttgt cagcggcccg gcggaatact gtcggcgctc ggcggcctgg tcgatccgtt
     1081 ccgcctcgcg cgaggactgt ttgcggcgag tcagcgccac ggagtccagc tctacgcgcg
     1141 cacgaaggtg gcgcagatcg aaacgaatcc cgaagcgctc gtactgcaca ccgaaggcgg
     1201 gaatacggtg acggcagccc atgtggccgt ctgcgccggc tacgagtctc tcgagttcct
     1261 gccgcacgac gtggccgacg tgaacaacac gtttgcgctg gtcaccgaac cgctgaggca
     1321 gcgcaaacag gcggacaccc tgccattggt ctgggaaagc gcgcgtcctt atctctatct
     1381 gaggggcacg ccggacgggc ggctcatgat tggcggcgcg gatgtgccgt tcaagaacgc
     1441 cggcgcccgc gatctgctgc tgccgcgcca ggtccggcgg ctgcaggagc agttccagga
     1501 cttgtttggc gccgagatgc cgcccgtcgc ctatgcctgg gcgggcagtt ttgccgagac
     1561 gcgcgacggt ttaccgctca tcggccgcgt ccccggcatg gatccgcggc tgcagttcgc
     1621 gctctgttat ggcggaaacg gcatcacgta cagcgtgcac gccggtgaaa tgattcgcgc
     1681 cggcatcgag ggccgggcgc acgacctcga cgacgttttc gggttcggga ggctgggaac
     1741 ggatctctcc gccggccggc aacggggaat cgctggaagt tagttcgacg tccggacaga
     1801 cgctggacac cgtgtcagcg ccgactctgc accgaattat gtggtctggc gtggatttgc
     1861 ctccgaaatg ggcctgacaa ctgttggcgg accgccgaac cccggaaatc ccgtggaatc
     1921 agacttgtgg ttattgtctc gctggcaccg ccgggacaat aattctgcaa atgccgaaaa
     1981 ccttttcggg ggatagggag actaaccccc aatgttcgta aatgacaccg gtaggctgcc
     2041 ggactccggg cgagtctgcg tgatgggttt cgcacccgac acgtcgggca cgaggacgta
     2101 ctacagcctg ctcatcaatg ctgcgcgtgc cgaggtcgag cccatgtcgg agcctcgcaa
     2161 cccggcgcac gtgctcggcg tgggcgccac catcgaatcg gtcaacggac cggtgttcgc
     2221 ggcgtacttc gcctcgtcgc gcgagcgctt cgacgcgcag cctaactaca gatcggggct
     2281 gttcgtgctg gtggacggcg ccgtgtacgc ggtcaaggcc gaacagatcg agaccagcat
     2341 ccggcgtggt ttcagtacgc gcgcgttcac gctgttgcgc ggcgaccggc cacagcgcag
     2401 cgtggtctat ccgtggccgt ggtatcgcga gccattcgtc tcgggtggta gcggcgtgcg
     2461 ctccggcgac ttcctgcgcg agatgtcgtc gatggtccgc gaatacggct gagcccgggc
     2521 ggctagccgc cggtcttctt ctccacgtga ccgccaaact gcttgcgcat cgctgacagc
     2581 agccggtcgc catagtcggc attgccgcgc gattcgaagc ggctgtagag cgccgcggtg
     2641 atcacgggcg ccggcacacc ttcatcgacc gcggccgcca ccgtccatcg tccttcgccc
     2701 gaatccgaaa cacgccccgc gaaagcgccg agaccggcgt cttcggacag cgcgcgcgcg
     2761 gtgaggtcca ggagccacga tcgaaccacg ctgccatggc gccacacttc cgcgatccgc
     2821 gcgagatcga attcgtacgc gtagtgctcg ggatgctgga gcggtgtcgt ctccgcgtcc
     2881 ttctcatgcg ttcgcgtgcc gatgttggca ttgcgcagga tgttgaatcc ttcggcgtag
     2941 gcggccatca ggccgtactc gatgccgttg tgaaccatct tgacgaagtg accggcgccg
     3001 tatggcccgc aatgcaggaa tccgcgcgca gcgctgtcgg gcccgtcgcc gccgggcgcg
     3061 agcgcggcga acagcggttc gagtgagtcg acgaccggtt tttctccacc gatcatcagg
     3121 cagtaaccgt tctcgccacc ggcgatgcca ccgctggtgc cgacgtccag gtagtggatg
     3181 ccggccttca acagttcgcc accgcgccga aggtcgtcgc gatagtagga attgccgccg
     3241 tcgatcagga cgtcgcctgc cgacagcaat ggacgcaggt tgttgatctc ctgctcgacg
     3301 atggccgcgg gcagcatgag ccagatcacc cggggcgcct tgagcgtttc cacgaactct
     3361 tttagcgacc cggtgccggt cacgccgggc ccatagagtg cccgcaccgc cgcgggattc
     3421 gcgtcgaata cgacgcactg aaatccggcg cccgtgagcc gccgcaccat gttggctccc
     3481 attcgtccca ggccgatgaa gccgacttct ttcatctctt tcatgtgcgc ctctcaatga
     3541 atgggtacag tctattccca tgaaagtcgt tcgacgcagc gcagatcgcg gtcatgccga
     3601 ccatgggtgg ctcaagtcct accacagctt ttcctttgcc gagtatttcg acgaggacca
     3661 cgtggagttc gggccgcttc gcgtgctcaa cgaagatcgc gtgatcggcg ggcagggctt
     3721 cggcacccat gggcaccgca acatggagat catcagctac gtgctcgagg gcgagctgac
     3781 acatcaggac tccatcggga caaggtcgac cattcgcccg ggtgacgtgc agcgcatgag
     3841 cgccggcacc ggtgtgcggc acagtgaatt caacgggtcg cagacgaaca agctgcactt
     3901 tttgcaaatc tggatccagc cgaacgcggc cgacatcccg ccgagttacg aagagaaaca
     3961 cttcgacgcc aatgacaaac gcggccggtt gcggttgatt gcttcatcag acggcgaagg
     4021 cggttcggtg ctcgtccacc aggacgtgcg ggtttacagc ggtctgttcg acgcgggcga
     4081 aagcgcgcgg ctcgaactcg cgccaggtcg tcgtgcgtac attcacgtgg cgcgcggtct
     4141 cgtgcacgcc aacggcgtgg agctcagcgc gggagatgca ctgaggctcg tggacgaaag
     4201 cgtcatcgag attgcgcaag ggtccaaatc agaagtcctg gtgttcgacc ttccgtagtc
     4261 actggactag tcaggaggga agggcatcgc cttcggcatg cgcgtgccgc catcgtgccg
     4321 cggtttgcgg gccttggcgt cgatgcctct ccaccacggc atcagcgcga att
//
LOCUS       DATDEU010000419         3860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_369574, whole genome shotgun sequence.
ACCESSION   DATDEU010000419 DATDEU010000000
VERSION     DATDEU010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369574"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="alaS"
                     /locus_tag="VIV63_10005"
     CDS             complement(<1..971)
                     /gene="alaS"
                     /locus_tag="VIV63_10005"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY6124977.1"
                     /translation="MSAADVRRAFLEFFRERGHTVVASSSLVPGNDPTLLFTNAGMVQ
                     FKDCFLGRDKRDYVRATSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     KDAIRFAWDFVTGTLKIDPARLMVTVYATDDEAFELWHQEAGVPKDKIVRIGDKPGGG
                     SDNFWQMGETGPCGPCTEIFFDHGEHIPGGPPGSPDADGDRWVEIWNLVFMQFDRSAD
                     GKLTPLPKPSVDTGAGLERVSAVMQGVTNNYDIDLFKNLVKAAAALANTTDLTNSSLR
                     VIADHIRACTFLVIDGVTPSNEGRGYVLRRIIRRAIRHGYKLGIKDA"
     gene            complement(1011..1604)
                     /locus_tag="VIV63_10010"
     CDS             complement(1011..1604)
                     /locus_tag="VIV63_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY6124978.1"
                     /translation="MRAPRGLADLFAGEQVTVRRPGGKKALAPEKAADPKSAHAFAIA
                     YLARRDFSASDLRRKLKDRGFDEGAIEPVLVELASSNVINDGRYGENVVAHRARRGQG
                     PARIRQELKRSGLGSEDIQATMDKAKDDGPDFVSLARAARARKFGSEIPKDWKERSRQ
                     ARFLQYRGFSTDHIRAVLEGVVDDESDADPGSEPEST"
     gene            complement(1556..2806)
                     /locus_tag="VIV63_10015"
     CDS             complement(1556..2806)
                     /locus_tag="VIV63_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6124979.1"
                     /translation="MRIRTLLFVLTALGSLPAQAADTWVIHAAKIYPAPYAQPVEDGV
                     VLVRDGRVTYVGKRESAKIPKSAKTSSCSGGVLTAGFQNSHVHFVETRWVDAARKPAA
                     ELEQSLQQMLTQYGFTTVFDTGSDQANTFALRARVQKGEIRGPRILTVGLPLFPPDGV
                     PSYINDLPPDFLARLHQPRTAEEARAAVRANIAAGADGTKLFLHTSPRQGVITKMSAD
                     IARAAAEETHARGKLVMAHPTSLEAIRLALDVGVDVLVHTTLGEASAWDADTLTRMKT
                     AHLSVVPTFKLWSYELKKEDVPPNVVDTLVGATLRELKAFNGVNGQVLFGTDVGYMRD
                     YDPTDEYVLMVKAGMTPGQILESLTTAPAVRWKEEQLHGQIANGFAADLVMLAADPFV
                     DVKNFANVKCVLRAGSPIYSRASK"
     gene            complement(2850..>3860)
                     /gene="recA"
                     /locus_tag="VIV63_10020"
     CDS             complement(2850..>3860)
                     /gene="recA"
                     /locus_tag="VIV63_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY6124980.1"
                     /translation="ALSAALGQIEKQFGKGSVMRLGDAAASYDVEAVSTGSLGLDIAL
                     GIGGLPRGRVIEIYGPESSGKTTLTLQVIAEVQKLGGTAAFVDAEHALDPSYAEKLGV
                     NVNELLVSQPDTGEQALEITDMLVRSSAVDVVVVDSVAALTPKAEIEGEMGEMQMGLH
                     ARLMSQALRKLTGNISRSNTMVIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDI
                     RRIGAIKNGEEVVGNQTRVKVVKNKVSPPFREAEFEIMYGMGISREGEIIDLGSAQGI
                     IEKSGSWYSYNGERIGQGKDNARDFLKSRPELAKEIENLIRAKLVPVKAPRPELKSVS
                     SD"
BASE COUNT          659 a         1172 c         1264 g          765 t
ORIGIN      
        1 aacgcatcct tgatgccgag cttgtaaccg tggcggatgg cgcggcgaat gatacgccgg
       61 agtacataac cgcggccttc gttcgaaggc gtgacgccgt cgatgacgag gaacgtgcac
      121 gcgcggatgt gatccgcgat cacgcgcaaa ctactgttcg tgagatctgt ggtgttcgcg
      181 agcgcggccg ccgcctttac gaggttcttg aacaggtcga tgtcgtagtt gttcgtgacg
      241 ccttgcatga ccgcggacac gcgctcgaga cccgcgccgg tatcaacgga cggcttcggc
      301 agcggcgtga gtttgccgtc ggccgagcgg tcgaactgca tgaacacgag gttccagatc
      361 tcgacccagc gatcgccatc ggcgtcgggt gagcccggcg gaccgcccgg aatgtgttcg
      421 ccgtgatcga agaagatttc ggtacacgga ccgcaggggc cggtctcgcc catctgccag
      481 aagttgtcgg agccgccgcc cggcttgtcg ccgatgcgca cgatcttgtc cttggggacg
      541 ccggcttcct ggtgccacag ctcgaaggct tcatcgtcgg tggcgtacac cgtcaccatg
      601 agacgcgccg ggtcgatctt caatgtgccc gtgacgaagt cccaggcgaa gcggatggcg
      661 tccttcttga agtagtcgcc aaacgagaaa ttgcccagca tctcgaagaa ggtgtggtgg
      721 cgggcggtat agcccacgtt ctcgaggtcg ttgtgcttgc cgcccgcgcg cacgcagcgc
      781 tggctggagg tggcgcgcac gtagtcgcgc ttgtcgcggc cgaggaagca gtccttgaac
      841 tgcaccatgc cggcgttcgt gaacagcagc gtcgggtcgt tacccggcac gaggctggag
      901 gacgcgacca cggtgtggcc gcgctcgcgg aaaaattcga ggaaggcgcg acggacgtcc
      961 gccgcgctca tgaaaggcgg tttctggggc atgggctcgg aagcaagggc ttaagtggat
     1021 tcgggttcgc tgcccggatc ggcgtctgac tcgtcgtcga cgaccccctc caaaacagcg
     1081 cggatatgat ccgttgaaaa gccccggtat tgcaaaaatc tggcctgccg cgagcgttct
     1141 ttccagtctt tggggatttc ggacccgaat ttccgcgccc gggcagccct ggccaggctc
     1201 acgaagtccg gaccgtcgtc cttggccttg tccatggtgg cctggatgtc ttccgaacct
     1261 agccccgacc gcttgagttc ctggcggatg cgagccggac cctgaccccg ccgcgcccgg
     1321 tgcgccacca cgttttcgcc gtagcggcca tcgttgatga cgttgctgga ggcgagctcg
     1381 accagcaccg gctcgatggc gccttcgtca aagccccggt ccttgagttt tcgccggagg
     1441 tcggacgcgg agaaatcgcg ccgcgccagg taagcgatgg cgaaagcatg cgcgctcttg
     1501 gggtccgccg ctttttcggg ggccagcgct tttttgccac ccgggcgccg cacggttact
     1561 tgctcgcccg cgaatagatc ggcgagcccg cgcggagcac gcacttcacg ttggcgaaat
     1621 ttttgacgtc cacgaaaggg tccgcggcca gcatgacgag gtccgcggca aatccgttcg
     1681 cgatctggcc gtgaagctgc tcttctttcc agcgcacggc gggcgcggtg gtgagcgatt
     1741 ccagaatctg ccccggcgtc attccggcct tgaccatgag cacgtattcg tcggtgggat
     1801 cgtagtcgcg catgtatccg acgtccgtgc cgaacaacac ctggccgttc acaccgttga
     1861 acgccttcag ctcgcggagc gtggcgccga ccagagtgtc gacgacgttc ggaggcacgt
     1921 cttccttctt caattcgtat gaccagagct tgaaggtggg cacgacggac aggtgcgcgg
     1981 ttttcatgcg cgtgagcgtg tccgcgtccc aggccgacgc ttcaccgagc gtggtatgca
     2041 cgagtacgtc gacacccaca tccagcgcca accggatggc ttcgagcgag gtgggatggg
     2101 ccatcaccag cttgccgcgt gcgtgagttt cttcagcggc agcgcgcgcg atgtccgccg
     2161 acatcttcgt gatgacgccc tgccgcggcg aggtgtgcag gaacagcttg gtgccgtcgg
     2221 cgccagcggc gatgtttgcg cggaccgcgg ctctggcctc ttccgcggtg cgcggctgat
     2281 gcaggcgcgc gagaaaatcc ggcggaaggt cgttgatgta gctcgggacg ccgtcgggcg
     2341 gaaacagagg aaggcccacg gtgaggatgc gcgggccccg tatctcgccc ttctgtactc
     2401 gcgcgcggag ggcgaaagta ttggcttgat ccgaacccgt atcgaacacg gtggtgaaac
     2461 cgtattgcgt cagcatttgc tggagggact gctccaattc cgccgcgggt ttgcgagcgg
     2521 cgtctaccca acgcgtctcg acgaaatgaa cgtggctgtt ttgaaaacca gcagtcaata
     2581 cgccgccgct gcagctcgac gttttggccg actttgggat cttggccgac tcgcgtttgc
     2641 cgacgtaggt gacccggccg tcgcgcacga gcacgacgcc atcttcgacg ggctgcgcgt
     2701 aaggagcggg atagatcttc gccgcgtgga tgacccaggt atccgcggct tgcgccggca
     2761 aactacccag cgcggtcagg acaaataaaa gcgtgcggat gcgcatgaga aacaggggca
     2821 gcgttaccgc tgcccctttc ccgaatatat caatcggagc tgacgctctt gagctcgggg
     2881 cgcggtgcct tcaccggcac gagcttggcg cggatgaggt tctcgatttc cttggcgagc
     2941 tccgggcggc tcttcaggaa gtcgcgagcg ttgtccttgc cctggccgat gcgttcaccg
     3001 ttgtagctgt accaggaacc cgatttctcg atgatgccct gggccgaacc caggtcgatg
     3061 atttcgcctt cgcgcgaaat gcccatgccg tacatgattt cgaattcggc ttcgcggaac
     3121 ggcggcgaca ccttgttttt gacgactttc acgcgcgtct ggttgccgac gacttcttca
     3181 ccattcttga tggcgccgat gcggcggatg tcgaggcgaa ccgacgcgta gaacttgagc
     3241 gcgttgccac cggtggtggt ttccggattg ccgaacatga cgccgatctt catgcggatc
     3301 tggttgatga agatgaccat ggtgttcgag cgggagatgt tgcccgtgag cttgcgcagc
     3361 gcctgcgaca tgaggcgcgc gtgcagaccc atctgcattt cgcccatttc gccttcgatt
     3421 tcagccttgg gcgtgagggc cgccaccgag tcgaccacca ccacgtctac ggcgctggag
     3481 cgcacgagca tgtcggtgat ttcgagggct tgttcacccg tgtccggctg ggagaccagc
     3541 agctcgttca cgttgacgcc gagcttttcg gcataggaag gatcgagcgc gtgttccgcg
     3601 tcgacgaacg ccgcggtgcc gcccagcttt tgcacttcgg cgatgacctg cagcgtgagc
     3661 gtggtcttgc cggaagattc cggaccgtag atttcgatga cgcggccgcg gggcagacca
     3721 ccgatgccga gcgcgatgtc gagacccagc gaacccgtgg agacggcttc cacgtcgtat
     3781 gacgcggcgg cgtcacccag acgcatgacc gaacctttac cgaattgttt ttcgatctga
     3841 cccagcgcgg ctgagagcgc
//
LOCUS       DATDEU010000420         9422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2121518, whole genome shotgun sequence.
ACCESSION   DATDEU010000420 DATDEU010000000
VERSION     DATDEU010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121518"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VIV63_10025"
     CDS             complement(<1..530)
                     /locus_tag="VIV63_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124981.1"
                     /translation="MFRKLFLAASVLVLSFSASAQVPVTDLAKPPAGATHYIIQSTGG
                     KHGDSWIWKTADGTRMGRESLNLRGQVFELDSTGKAGADGMPESVAIRGVTPQGDATE
                     TFSVTNGKASWKSPVDAGSAAYSKPVFYSTQGGPIDTSAWFIERLLENPDHTLPLLPG
                     GKAKAEKLTTLTVGEGA"
     gene            757..4065
                     /locus_tag="VIV63_10030"
     CDS             757..4065
                     /locus_tag="VIV63_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124982.1"
                     /translation="MDVRRYLAIAGALVLGACIHADAAAQAVPPAPVTAAVKPAPAQV
                     DPNSSLSRLYPGAGSIGMAVAIAPDPNVPRYRRLFDLGIHAITLGMLHDGYVLDRYSI
                     PWSAGTGTVGDPDPGAFGIMIFRCDGWREHACRDSATPSSAGIRSKQTTRVRAIYIVT
                     DTATWGVATRPLTCAARRIRAQLSGVEPEENADCVPESPVAARALGPPGKTRAELLQY
                     PGRCESADGRKPLVLFGPNFSGGMDSVGQHVAKILGPGITDVCLVSSTTTNSSNPLVE
                     DAYGHLSYVQLAASDGTKLLRLADVAESFGYFDPSGNTARGTPGNRKYDVAFLTEAST
                     FGYGVCNPWEQKDPAKLARIQDFCRDAYTLHFPAAVADIRYGIEQQRERQQNSVQTAM
                     KAALPSEHLTLDVGAENGSEFPENRQSKLTAASQQLALDHVLEQLEQFAPKMVIVVAT
                     DVRDRLFLFDQLRQRLPSAMLIDLETDILLGHPDFLHASRGAVTVASANLFVRRGRLF
                     GCEEINTETKVRKRVPLASWALDGQGILAGAIGRLFDSGQPRTDLPCIHQPELEKKFG
                     ARAPILHVVTLKGLRRISSVSDLHPDPDKPYTKSRKLQQTLVDVGQWLSIPCCLVIVL
                     PWLWIGPLRKSDKPVLAHDPWKVTLAAAAAGAIVWTFAMQAAYLGVDPESGFALLYWS
                     VGILSLALAGLFQCLKRVQESGQIVGELPRRHRIAIASVGLFACALAAAPLFSCYRHA
                     KPLPATSDVPPIDVSLMTRLALDIGQGLAFEVVIALGVVVVLAIMVGLATGLCILVRN
                     NQLLEITNGCRALEVGARTPKFSVAPVVGIVLLIAIIAVPSLVPALGGPRLTVFGPTA
                     SLMATIVLVATTLGASIFTVVAIHSSRRVRTISAHIGRCVAPPPEPGQTEPPAGEYPG
                     LWRANEWQPDLFATTPVVASVGADIIASLNADQERPWKILINEFLGKCDGGKRSDDGK
                     HRRAIYALLASEISLYRWLVAGAVLCASASVCAAYLFPLEADALLLWNLLVLVVHALL
                     AGYVATAFERDGVLSNILCNRPKEAKFSASLFTYAALPFFALGFAIAVSQVPGVVDWG
                     GGLLVLLGAIGLGP"
     gene            complement(4070..4465)
                     /locus_tag="VIV63_10035"
     CDS             complement(4070..4465)
                     /locus_tag="VIV63_10035"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY6124983.1"
                     /translation="MNDKTVAAYDKAQKPAQAAICASLRKSFDAALPKATSKVWHGSP
                     VWFIGENPVVGYTARPAGVAVLFWNGKEFDEPALEPVGKFHAAQIKFADLGAIDAKLL
                     ARLLRKAKTDIWDSVGWRKRMLAKKKRGG"
     gene            complement(4471..4905)
                     /locus_tag="VIV63_10040"
     CDS             complement(4471..4905)
                     /locus_tag="VIV63_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY6124984.1"
                     /translation="MRVMVIVKATKNSEAGIMPSEQMFLDMGKFNEELVKAGIMQFGD
                     GLQPSVKGKRIHFAGGKHSVIDGPFAETKELIAGFWIWKVKSMEEAVAWASRCPDPMP
                     GEESTLEIRPFFEMEDFGESATPKVKENVEKLRAEMERQQAK"
     gene            5027..6154
                     /locus_tag="VIV63_10045"
     CDS             5027..6154
                     /locus_tag="VIV63_10045"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6124985.1"
                     /translation="MQLSRRALLFTSAVLATGAHAAPKKQLQSARGVVQEILAANAIP
                     ALSIAVMRGDELLWAEAFGKVDLELDVAATPAHRFRLGSVSKIFAATLAAVLAARGVV
                     DLDAPIATYMPALPDQHRATTLRQLLTHRGGVRHYTAKDESAEPPGPIDRRQYLSNAD
                     ILAVFIDDPLIARPGERVSYSTFGYTLASVVLETAAKLPFLELLQRDIATPLGLASLS
                     GDAPMLIVPNRVSGYHPGDLVRKAFPTFEGKWSNTAQNNPAYKWAGGGLLATPSDVAR
                     FGAAHLAPGKLSKAALDTLFTVYTERNERSPPLGLGWRIDEDAAKRLRWHHAGGQDGA
                     RASLVVYPKEKLSIAFATNVTQTPGDVNGPSAKIADAFLAA"
     gene            6336..6935
                     /locus_tag="VIV63_10050"
     CDS             6336..6935
                     /locus_tag="VIV63_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124986.1"
                     /translation="MQISKAFLALAAASLLFTGGCAKQKAPATKALESVETSLNAVRE
                     DAQKYAAEGLKGVEAQYARLKESLDKKEYDNVLAGAPQLEKAVASLKDAVDAGQQHAK
                     AALAAAKSEWAALNAEVPQMIEGIQTRVDEQSKKKIRFKVSKEELESAKSGLEWMKTE
                     WAQATAALDSGNQIKAADMAKAVKAKGEEVQKTLGMKEA"
     gene            6935..7534
                     /locus_tag="VIV63_10055"
     CDS             6935..7534
                     /locus_tag="VIV63_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124987.1"
                     /translation="MHFSKALTSLVAAMAATTILATGCASQEEPAAQAVIAAEASLAE
                     VRVDAAKFAPEELKAAEAKLAKLKSELAKDDYKDVLAGSSQLTQEVATLKEVVVSKQT
                     QAAAATHEWESLSAEVPKMVEAIQSRVDTLSGSRKLPADVNKEAFEAAKSALASMKSM
                     WAEASAAFDAGNAVVAADKGRLVQAKGEEVLGQLGMSPV"
     gene            complement(7543..8466)
                     /locus_tag="VIV63_10060"
     CDS             complement(7543..8466)
                     /locus_tag="VIV63_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6124988.1"
                     /translation="MPDLADLETFVAVADAGGVSGAARRLGLPKSIVSRRLARLEGEL
                     NAQLLTRTTRGAALTEAGATFREHAARMVAELEAARESLSPDGELRGLLRIAAPLSFG
                     ASHLAPLIADFAARHPQLQITTSYGDRFVDLVREGFDVAIRLGWLPDSSLVARRIGPV
                     TGRLVATPDYIAKHGAPTSLDDIAKHPALMQGTEIWRVRDGDQVVTLHPQGRFKADNG
                     QALVSAVLAGLGIAMLPDFLIDEHIASGALVTLLPQYPMPDAGLYVVRPPGDHPSRKV
                     RVFTDMLVERLSVQCTVAPGMLQLPGRHASS"
     gene            8566..9192
                     /locus_tag="VIV63_10065"
     CDS             8566..9192
                     /locus_tag="VIV63_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEY6124989.1"
                     /translation="MSNILVINSSAAGRDSVSRVLVADAVSKLLEVNPNAKVTHRDLG
                     ENPPPHLISQTLNGVRGEAVTEVEFAARRLSDELVGELKAADTVVIGAPMYNFSVPTT
                     LRAWFDHVLRAGVTFKYADGAVRGLLGGKRVIVIESRGGLYSEGPAQAVDFQEPYLRH
                     LLGFIGITDVTFVHAEKIGYGPEARTAALETAKAKISEVVGAELVKAA"
BASE COUNT         1680 a         3095 c         2955 g         1692 t
ORIGIN      
        1 gcaccttctc ccacggtgag tgtggtcagc ttctcggctt tggccttgcc accgggcaac
       61 agcggcagcg tgtgatcggg attctcgagc agccgctcga taaaccacgc cgacgtgtcg
      121 atgggcccgc cctgcgtcga atagaagacg ggcttcgaat aagccgcgct tcccgcatcc
      181 accggacttt tccagctcgc cttgccattc gtgacgctga aagtttccgt ggcgtcgccc
      241 tgcggtgtaa cgccgcggat cgcgacagat tcgggcattc catctgcacc cgccttgcca
      301 gtcgagtcga gttcgaaaac ctgcccgcgc agattcaggc tctcgcggcc catgcgggtg
      361 ccatcggcgg tcttccagat ccaggagtca ccgtgtttgc cgcctgtcga ctggatgatg
      421 tagtgcgttg caccggccgg cggcttcgcg agatctgtaa cgggtacctg cgccgaagca
      481 gagaaagaga gtacgaggac cgaagcggcg agaaagagct tgcggaacac gggaatctcc
      541 caagagtgat ttgaccctga ggataaccgc ccagggacgc actttcactg cattcctgct
      601 gccacgcggc gcgaaatcgc ttcgtctcgt ctcgaaattt tgggggcttt cgtcacagtt
      661 gacgccgcgc cctccgtgtg cgggcttgtt tgcgcggcgc acgcgcgccc aggctcgaga
      721 cggcgatgct ctaactacat tgcggccggt tcgaatatgg atgttcggcg atatctcgcg
      781 attgcaggcg ccctggttct cggggcctgc atccacgcgg atgcggcagc gcaggcggtg
      841 ccgccagcgc cggtaacggc ggctgtaaaa cccgcacccg cgcaagtgga ccccaacagc
      901 agcctgtcga ggctgtatcc cggggccggc agcatcggca tggctgttgc gatcgcgccc
      961 gatccgaacg tgccgcgtta tcgaaggctc ttcgacctcg ggatccacgc catcacgctc
     1021 ggcatgctgc acgacggtta cgtgctcgat cggtattcga ttccgtggag cgccggcacc
     1081 ggaaccgtcg gcgatccgga tccgggagca ttcggcatca tgatcttccg ttgcgacgga
     1141 tggcgcgaac atgcgtgccg cgattccgcg acgccctcgt ccgccggcat cagatcgaaa
     1201 caaactaccc gcgtccgcgc catctacatc gtgacggaca ccgcgacctg gggcgtggcg
     1261 acacggccac tcacgtgcgc ggcccgccga attcgcgccc agctatcggg cgtcgagccc
     1321 gaagagaatg cggattgcgt cccggaatcg cccgtggccg ccagggcact gggaccgcca
     1381 ggaaaaacaa gagccgaatt gctgcagtat cccggccgtt gcgagtccgc cgatgggcgc
     1441 aaacctctgg tcctgttcgg ccccaacttc tccggtggca tggactcggt gggacaacat
     1501 gtcgcgaaaa tcctggggcc cgggatcacc gacgtctgcc tggtctcgag caccacgacc
     1561 aacagctcca atccactcgt cgaggatgcg tacggccatc tgagctacgt ccagctggcc
     1621 gcgagtgatg gcaccaaact actgcgcctc gccgatgtcg ccgagtcttt cggctatttc
     1681 gatccgtcgg gaaacaccgc tcgcggaacg ccgggaaacc ggaagtacga cgtcgcattc
     1741 ctcaccgaag cgtcgacgtt tggatatggc gtctgcaatc catgggagca gaaggatccc
     1801 gcgaagctcg cgcgcatcca ggatttctgc cgcgatgcgt acacgcttca ctttccggcc
     1861 gcggtcgccg acatccgcta cggcatcgag cagcaacgcg agcgacagca gaacagcgtg
     1921 cagacggcga tgaaggccgc cctgccgagc gaacacctga cactcgatgt aggtgcggaa
     1981 aacggcagcg aattcccgga gaaccggcag tcgaagctca ccgcggcgag ccagcagctc
     2041 gcgctcgatc acgtgctcga gcagctcgag cagttcgcgc cgaagatggt gatcgtggtg
     2101 gcgaccgacg tgcgcgaccg gctgttcctg ttcgatcagc ttcgccagcg cctgccgagc
     2161 gccatgctca tcgatctcga gaccgacatc ctgctcgggc atccggattt cctgcatgcc
     2221 agtcgcggcg ccgtcaccgt cgcttcggcg aatctcttcg tgcgccgggg ccggctcttc
     2281 ggctgtgaag aaatcaacac tgaaaccaaa gtccggaaac gcgtgccgct ggcgtcctgg
     2341 gcactcgacg gccagggcat tctcgcgggc gccatcggac gcctgttcga ctccgggcag
     2401 ccgcgcacgg atctgccgtg catacaccag ccggagctgg agaagaagtt tggtgcgcgt
     2461 gcgccgatcc tgcatgtcgt cacgctcaaa ggtttacgcc gcatcagttc cgtttccgac
     2521 ctccatcccg atcccgataa accgtacacg aagtctcgga aactccagca gacgctcgtc
     2581 gatgtcggcc aatggctgtc gattccttgt tgcctggtca tcgtcctgcc gtggttgtgg
     2641 ataggcccgc tgcgcaagtc cgacaagccc gtgctcgcgc acgatccatg gaaagtgact
     2701 ctggccgccg cggccgcggg cgcaatcgtc tggacgttcg ccatgcaggc ggcatacctc
     2761 ggcgtggatc ccgaaagcgg gtttgcgctg ctgtattggt cggtcggcat cctgtcgctc
     2821 gcgctcgccg ggctctttca atgtctgaaa cgggtgcagg agtcggggca gatcgtcggc
     2881 gaattgccgc ggcgccatcg catcgccatc gcctcggtcg ggttgtttgc ctgcgcgctg
     2941 gccgcggcgc cgctgttctc ctgctatcgg catgcgaagc cgctgccggc aacgtcggat
     3001 gttccgccca tcgacgtgtc gctgatgacg cggctcgcgc tcgacattgg ccaggggttg
     3061 gcgttcgagg tggtcatcgc gctcggcgtc gtcgtggtgc tcgccatcat ggtcgggctt
     3121 gccaccggtc tctgcatcct ggtgcgcaac aaccagttgc tcgaaatcac caacggctgc
     3181 cgtgccctgg aagtgggcgc tcgcacgccg aaattttcag tggcgccggt cgtcggcatc
     3241 gtattgctca tcgcgatcat cgcggtcccc agcctggtgc cggccctcgg cggtccgcgt
     3301 ctgaccgtgt tcggaccgac ggcgtcgttg atggcgacca tcgttctcgt cgcgaccacg
     3361 ctgggcgcga gcatcttcac ggtcgtcgcc attcactcga gccggcgcgt acgaaccatc
     3421 tcggcacaca tcggccgctg cgtggcgcct ccgcccgaac ccggccagac cgagccgccg
     3481 gcgggcgaat accccgggct gtggcgagcg aacgagtggc aacccgacct cttcgcgacg
     3541 acacccgtcg tcgccagtgt gggcgccgac atcatcgcga gcctgaacgc cgaccaggaa
     3601 cgcccctgga agattctgat caacgaattc ctcggcaagt gtgatggcgg caaacgcagc
     3661 gacgacggca aacaccgccg cgccatctac gcattgctcg cgagcgagat ctcgctgtat
     3721 cgctggctgg tcgccggcgc cgtgttgtgc gccagcgcca gcgtgtgcgc cgcctatcta
     3781 tttccgctcg aggcggatgc gttgctcctg tggaacctgc tggtgctcgt cgtgcatgcg
     3841 ctgctcgccg gctacgtcgc cacggctttc gagcgcgatg gcgtgctcag caacattctt
     3901 tgcaaccggc cgaaggaagc caagttctcc gcatcgttgt tcacttacgc cgcgctgccg
     3961 ttcttcgcgc tcggcttcgc gattgccgtc tcgcaggtgc ccggcgtcgt cgattggggc
     4021 ggcgggttgc tggtcctgct cggcgcgata ggcctgggac cttgagccct cagccgccgc
     4081 gctttttctt cgcgagcatc cgcttgcgcc agccgaccga gtcccagatg tccgtcttcg
     4141 ccttccggag caggcgggct aacaacttcg cgtctatcgc gcccaagtcg gcgaacttga
     4201 tctgcgcagc gtgaaacttg cccaccggtt cgagcgcggg ttcatcgaac tccttcccgt
     4261 tccagaacaa cacggccacg cccgcaggcc tggccgtgta acccaccacc ggattctcgc
     4321 cgatgaacca cacgggactg ccgtgccaca ccttcgatgt ggctttcggc agtgccgcgt
     4381 cgaacgactt gcgcaggctg gcgcagatcg ccgcctgcgc gggcttttgc gccttgtcgt
     4441 aggcggcgac cgttttgtcg ttcacgggtt tcacttcgcc tgctgccgtt ccatctcggc
     4501 gcgcagcttc tcgacgtttt ccttcacttt cggcgtagcg gactcgccaa agtcttccat
     4561 ctcgaagaag ggacggatct cgagcgtgga ttcttcgccc ggcatcgggt ccgggcagcg
     4621 gctcgcccag gcaacggcct cttccatcga cttcactttc cagatccaga aacccgcgat
     4681 gagctccttg gtctccgcga acggaccgtc gatgacactg tgtttgccac cggcgaaatg
     4741 gatgcgtttg cccttcacgc tgggctgcag cccatcgccg aactgcatga tgccggcctt
     4801 gaccagctcc tcgttgaatt tgcccatgtc cagaaacatc tgctcgctgg gcatgatccc
     4861 ggcttcgctg ttcttcgtgg ccttcacgat caccatgact ctcattgctt gctccttgtg
     4921 gatgttcgca aggtagtcga acggcatttc gccaattcga catgcaaccc gcacccgatt
     4981 ttccgcatca taagtccgcg attccattca ttttggagaa tccttcatgc agctgtcccg
     5041 ccgtgccctg ttgttcacca gcgccgttct cgccacgggc gcccacgcgg caccgaaaaa
     5101 gcagctccag tccgcccgtg gtgtcgtgca ggagatcctc gccgcgaacg ccattccagc
     5161 gctgtcgatc gccgtcatgc gcggcgatga gctgttatgg gccgaagctt tcgggaaagt
     5221 ggacctggaa ctcgacgtcg cggcgactcc cgcgcatcgg ttccgcctgg gcagcgtctc
     5281 gaagatcttc gccgcgacgc tggcggctgt cctcgccgcg cgcggtgtcg tcgacctcga
     5341 tgcacccatc gcgacctaca tgccggcatt acccgaccaa caccgcgcca ccacgctgcg
     5401 acagttgctt acgcatcgcg gcggcgtccg tcactacacg gccaaagatg agtccgcgga
     5461 gccgccgggc cccattgacc gccgccagta cctgagcaac gcggacatcc ttgccgtgtt
     5521 catcgacgac ccgctgatcg ccagacccgg cgaacgcgtg tcgtacagca ctttcggtta
     5581 cacgctcgcg tccgtggtgc tcgagaccgc cgccaaacta ccgttcctcg aactcctgca
     5641 gcgtgacatc gcgacgccgc tgggacttgc gtccctgagt ggggacgcgc ccatgctgat
     5701 cgtgcccaac cgcgtgagcg gatatcaccc cggcgatctc gtgcgcaagg cgttccccac
     5761 attcgaaggg aaatggagca acacggcgca gaacaatccc gcgtacaaat gggccggagg
     5821 cgggttgtta gccacgcctt ccgatgtcgc gcgcttcggc gccgcgcatc ttgcgccggg
     5881 aaagttgtcg aaggccgcgc tggacacgct cttcaccgtc tacacggaac gcaatgagcg
     5941 ctccccgccg ctcggtctcg gctggcgcat cgatgaagac gccgccaaac gcctgcgctg
     6001 gcaccacgcc ggcggacagg acggcgcccg cgccagcctg gtcgtgtatc cgaaggaaaa
     6061 gctctccatc gcgttcgcga cgaacgtgac gcagacgccc ggcgacgtga acgggcccag
     6121 cgcgaagatc gccgacgcat tcctggccgc ataggaaaac gtcgccgaat ggcgccactg
     6181 ttgcaggcgg ggcccgcctg gcaggcttat tagcctcggg cggaaactga cggcgatttg
     6241 gccatggata tagagtccat gtcctacgcg ggtaatgact ccttcgcgcc taacctagca
     6301 atgtccaggc tctgtgcagc ctgaaggagt cattcatgca aatttcaaaa gcgttcctgg
     6361 cgcttgccgc tgcctcgttg ctcttcaccg gcggctgcgc caaacagaaa gctcccgcca
     6421 ccaaggcgct ggagtcggtc gaaacctcac tcaatgcagt gagggaagat gcgcagaagt
     6481 acgcggcgga aggcctcaag ggcgtcgaag cgcaatatgc gcggctcaaa gagagcctcg
     6541 acaagaagga atacgacaac gtgcttgccg gtgcgccgca actcgagaag gccgtcgcct
     6601 cgctcaagga tgccgttgac gctggtcagc aacacgccaa ggccgcgctg gccgccgcga
     6661 agagcgagtg ggccgcgctg aacgccgaag tgccgcagat gatcgaaggc attcaaacgc
     6721 gtgtcgacga acagtcgaag aagaagatcc gcttcaaggt cagcaaggaa gaactcgaat
     6781 ccgccaagtc ggggctcgag tggatgaaga ccgagtgggc gcaagccacg gccgcgctcg
     6841 attccggcaa tcagatcaag gctgcggaca tggccaaggc cgtgaaggcc aaaggcgaag
     6901 aagtgcagaa gacgctcggc atgaaggagg cgtgatgcat ttttccaaag cactcacctc
     6961 tctcgtcgca gcgatggccg cgaccaccat cctggccacc ggttgcgcca gccaggagga
     7021 acccgcggcc caggccgtga ttgcggccga agcgtcgctc gccgaagtgc gggtcgatgc
     7081 cgcgaagttc gcgccggaag agctcaaggc cgcggaggcc aagctcgcga agctcaagag
     7141 cgaactcgcg aaggacgact acaaagacgt gctcgccggt tcgtcgcagc ttacgcagga
     7201 agtcgccacg ctcaaggaag tcgtcgtttc gaagcagacc caggcggccg ccgccactca
     7261 tgaatgggaa agcctgagcg ccgaagtccc caagatggtc gaagccatcc agagccgcgt
     7321 cgacacgctg tccgggtcac gcaagctgcc ggctgacgtg aacaaggaag cgttcgaagc
     7381 tgccaagtcg gcgctcgcgt cgatgaagtc catgtgggcc gaagccagtg ccgcgttcga
     7441 tgcgggcaat gccgtcgtcg ccgcagacaa gggccgcctg gtccaggcga agggcgagga
     7501 agtgctcggt cagctgggca tgagcccggt ctgatccgtt gactagctag atgcgtggcg
     7561 cccgggaagc tgcaacatcc cgggcgccac ggtgcattgg acgctcagcc gctccaccag
     7621 catgtcggtg aatacgcgca ccttgcgcga cgggtgatcg cccggcggcc tcaccacgta
     7681 gagcccggcg tccggcatcg ggtattgcgg gagcagcgtg accaacgcac ccgaagcgat
     7741 gtgctcgtcg atgaggaagt ccggcagcat cgcgatgccg agtcccgcca gcaccgccga
     7801 caccaacgcc tgaccgttgt ccgccttgaa gcgaccctgc ggatgcagag tgacgacctg
     7861 gtcgccgtcg cgcacgcgcc agatttccgt gccttgcatg agcgcgggat gtttggcgat
     7921 gtcatcgagc gaagtgggcg cgccgtgttt cgcgatgtag tcaggcgttg ccaccaggcg
     7981 tcccgtcacg ggaccgatgc gccgcgcgac gagtgaggag tcgggcaacc accccaggcg
     8041 aatcgcgacg tcgaaccctt ctcgcacgag atcgacgaac cgatcgccgt aagaagtcgt
     8101 gatctgcaac tgtgggtgac gcgcagcgaa atccgcgatc agcggcgcga gatgcgaggc
     8161 gccgaaagac agcggcgccg cgatgcgcag caggccgcgc aactctccgt cgggggacaa
     8221 actttcacgt gccgcctcca gctccgccac catgcgcgcc gcgtgttcgc ggaacgtcgc
     8281 ccctgcttcg gtcagcgccg cgccgcgagt cgtgcgcgtc agcagctggg cgttgagctc
     8341 gccctccagc cgcgcgaggc ggcggctcac gatggacttc ggcagcccca gccggcgcgc
     8401 cgcgcccgac acgcccccgg cgtcggcaac agccacaaag gtctctaaat cggctaaatc
     8461 gggcatggcg ttccagtttg tgcaacacag aaggtcattt ggcgtggcta gtgtgccggt
     8521 ctgcggaacg agagtatggg ctccaacaga taaggagcaa ggcccatgtc gaacattctc
     8581 gtgatcaaca gcagcgccgc cgggcgggat tccgtctcgc gagtgctcgt cgcggacgcc
     8641 gtttccaagc ttctcgaggt caatccgaac gccaaggtga cgcaccgcga tctcggcgag
     8701 aacccaccgc cgcatctcat ttcgcagacg ctgaacggcg tgcgcggaga ggcggtgacc
     8761 gaggtcgaat tcgcagcgcg tcgcctgtcc gatgaactgg tcggcgaact caaggccgcg
     8821 gataccgtcg tgattggcgc gcccatgtac aacttcagcg tgcccaccac gctgcgcgcg
     8881 tggttcgacc acgtgctgcg cgcgggcgtc acgttcaaat acgccgacgg cgccgtgcgc
     8941 gggttgttag gcggcaagcg cgtcatcgtc atcgagtcgc gcggcgggct ttacagcgaa
     9001 gggccggcgc aggccgtcga cttccaggag ccatacctgc ggcacctgct cggcttcatc
     9061 ggcatcaccg acgtgacgtt cgtgcatgcc gagaagattg gctacgggcc cgaggcacgc
     9121 acggcggcgc tcgaaacggc gaaggcgaag atttcggagg tggtgggcgc ggagctcgtg
     9181 aaggcggcgt aagtagttcg gccggctacg ggcgcgcagg cagttttact gcttgcgcgg
     9241 cccggagccc atgaggccga ggatggcctc gtacacctgg tcgaactgct ttcgggtgtc
     9301 ggcgtcgagc gcggctacgg agcgttccaa tgcttccatt tttcgggcga gcgccgtggt
     9361 cgatgccaag gactgacgca gttttacgaa cgcgcggata ttggcctgta tttacgatgt
     9421 tt
//
LOCUS       DATDEU010000421         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1392857, whole genome shotgun sequence.
ACCESSION   DATDEU010000421 DATDEU010000000
VERSION     DATDEU010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392857"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VIV63_10070"
     CDS             <1..434
                     /locus_tag="VIV63_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6124990.1"
                     /translation="AVMGIESFRREMDRRGAFYDRVTKYSQAFVSMLMQSVACNGLHS
                     AEQRCCRWLLTTHDRIGQDEFPLTHEFLAIMLGVRRPTVTLVMAELTRQGVLSHVRGH
                     VRITDRKGLEKASCECYRNVRAAFDRLLGAPPERHDVAVGQ"
     gene            415..1362
                     /locus_tag="VIV63_10075"
     CDS             415..1362
                     /locus_tag="VIV63_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY6124991.1"
                     /translation="MSQSDNKLLASLPASEYQRLRPLLRTLHLPAETGLPHCGQTRVY
                     FPGTGLCSIINKMADGSAIEVACIGSEGVVGSQGLSDDSPHDRNGFVQVADGTVQYLP
                     SVLFERELARNGRFRELIDTFSHAFLETMIQAVACNRLHTLEERCCRWLLGVHDRLGR
                     ARFELKVRFLARAMGVKNSAVAGIMASLEDQGLASHDGSSVTILDSIGLRRLACRCYD
                     AMKRGYTLERLAAQPRVEGPHRVARVLTMRQGVGACTLCGSSTRVPHRNGHECILALD
                     EEIDFLVQRTHLLRKYRGQLMANRAQLYRDILKRSNSRI"
     gene            1514..2107
                     /locus_tag="VIV63_10080"
     CDS             1514..2107
                     /locus_tag="VIV63_10080"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY6124992.1"
                     /translation="MAAENLQELFVEELRDVYDAEKQITKALPKMAKAVESDELRAAF
                     EEHLDITRMQINRLEEVFKSLGLAARGKTCDGMKGLLEEGQSHMEELDGSTLDAALIG
                     AAQRVEHYEMAAYGTLATWADLLGYQDAKDLLGQTLEEEKEADEKLTGIAGQINPEAE
                     PEEESDGSEQRNNGMAMVGSRRSGSTSGGRGRRGKKR"
BASE COUNT          425 a          620 c          691 g          387 t
ORIGIN      
        1 cggccgtcat gggcatcgag tcgttccgtc gcgagatgga ccggcgcggt gcgttttacg
       61 atcgcgtcac gaaatattcg caagcgttcg tgagcatgct catgcagtcg gtggcgtgca
      121 acggcctgca ctccgccgaa cagcgctgct gccgctggct tctgacgacg cacgatcgga
      181 ttggccagga tgagtttccg ctcacccacg agttcctcgc gatcatgctc ggagtgaggc
      241 gccccacagt gacgctggtc atggccgagc tcactcgtca gggcgtcctt tctcacgtcc
      301 gggggcacgt gcgcatcacc gatcggaagg gcctcgagaa agcgtcctgt gaatgctacc
      361 ggaatgtgcg ggcggcgttc gaccggctgc tgggcgcgcc gccggagcga cacgatgtcg
      421 cagtcggaca ataagcttct agcatcgctg ccggcgagcg aatatcagcg cttacggccg
      481 ctgctgcgta ctctgcatct ccctgcagag acggggttgc cgcactgcgg gcaaacccgt
      541 gtctattttc ccggcacagg tctctgctcg atcatcaaca agatggccga tggcagcgcg
      601 attgaggtcg cctgcatcgg aagcgagggt gtcgtcggca gtcagggact ctcggatgac
      661 agccctcacg atcggaacgg gttcgtccag gtggctgatg gtacggtcca gtacttgccc
      721 tcggtattgt tcgagcgcga gttggcgcgc aacggacgtt ttcgtgaact gatcgacacg
      781 ttctctcatg catttctcga gacgatgatc caggcggtgg cctgcaatcg cctccacacg
      841 ctcgaagagc ggtgctgccg atggctgctc ggcgtgcacg atcgcctcgg ccgcgccagg
      901 ttcgagttga aggttcgctt cctggcgagg gcaatggggg tgaagaactc cgcagttgcc
      961 ggaattatgg cctcacttga agatcagggg ctcgcgagcc atgacggatc gtcggtcacg
     1021 atcctcgaca gcatcgggct gcgccgcctt gcttgccgat gttacgacgc gatgaaacgc
     1081 gggtacaccc tcgagcgtct cgccgcgcaa cccagggttg aggggccgca tcgcgtggcg
     1141 cgcgtcttga caatgcgcca aggcgtcggc gcctgcacac tgtgcggctc gtcgacgcgg
     1201 gttccgcacc ggaacggcca tgagtgcatc ctggcgctgg acgaagaaat cgatttcctc
     1261 gttcagcgaa cgcacctcct tcgaaagtat cgcggccagt tgatggcgaa tcgcgcgcag
     1321 ctgtaccgcg acatcctcaa gcgatcgaac agccgcatct gaagtagcac cattgagaca
     1381 cacgtgatgc ccgctgctgc cgcagcgtac gcgtcggtgc tggcgtacgt cttgctgaat
     1441 attcctggcg tggcggctgt gtccgccaga gacccaatcg gcgcgaaagg cgcgcccttc
     1501 gaaaggaatc acaatggcag ctgaaaatct tcaggaattg tttgtcgagg aactccggga
     1561 cgtgtacgac gccgaaaagc agatcacaaa ggccttgccg aagatggcca aggccgtgga
     1621 aagcgatgaa ctccgcgcgg cgttcgaaga acacctcgat atcacccgca tgcagatcaa
     1681 ccgtctcgaa gaagtgttca agtcgcttgg cctcgctgct cgcggcaaga catgcgatgg
     1741 gatgaagggc ctgctcgaag aaggccagtc tcacatggaa gagctcgacg gttcgacgct
     1801 cgacgcggcg ctcatcggcg cggcacagcg ggtcgaacat tacgagatgg ccgcctacgg
     1861 tacgttggca acgtgggccg acttgcttgg gtatcaggac gccaaggatc tgcttggaca
     1921 gacgctcgaa gaggaaaagg aagcggacga gaagctgacg ggcatcgccg gacagatcaa
     1981 ccctgaggct gagcccgaag aagagagcga cggaagcgag cagcggaaca acgggatggc
     2041 aatggtcggc tcgcgccgca gtggttcgac cagcggcggt cgcggacgaa gagggaagaa
     2101 gcgctagatc cgttgtagcg atc
//
LOCUS       DATDEU010000422         4817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1392888, whole genome shotgun sequence.
ACCESSION   DATDEU010000422 DATDEU010000000
VERSION     DATDEU010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392888"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VIV63_10085"
     CDS             complement(<1..1210)
                     /locus_tag="VIV63_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEY6124993.1"
                     /translation="MEGRIEPLHSREVTALSTVDSPVVFAVKSLLLPIIPVITLWICM
                     AAANEQLRGGYFLIAVLTFLGAPDVLGLARISASDEQRPQGALLLEITARWITLVALI
                     AAVVYLAGVAHTLDLALLINWFVFTPAVLFVGQFAARQWLTHSLRGRRPQRAVIVGAT
                     ELGARLEGALRADSLLRTDIVGYFDDLPRPYLTANFTAPLLGDTNGLAEYVTHNQIDQ
                     VFITLPMSRDARIVAMLEALHDSTASIYFVPDIFAFNLIQARFDILGGVPVVAVRETP
                     FYGAAWLVKRLSDIAIAASAVLLLTPLLLAVAVAIRLDSPGPVFFRQKRYGLDGREIL
                     VLKFRSMTVTEDGKQTFKAAARGDSRITRIGALIRRTSIDELPQLFNVLEGSMSIVGP
                     RPHPVAMNEHY"
     gene            complement(1224..1493)
                     /locus_tag="VIV63_10090"
     CDS             complement(1224..1493)
                     /locus_tag="VIV63_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124994.1"
                     /translation="MPLLSLQGVHMKYLAILFLSLAASFTARTAPSAGAEPNVSMYAP
                     VLVRGAPAPSDGSSDRALAVVLAAALIALQLRRRQKSMRMPRQFR"
     gene            1952..3367
                     /locus_tag="VIV63_10095"
     CDS             1952..3367
                     /locus_tag="VIV63_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6124995.1"
                     /translation="MEQTLPTPATTKASASSPLIAEGLAVVFAVLVLPVFLYVAHLAP
                     LGRLLYPVANFLLAGYLFSRRSPWYASHCLLVFCFMSLARRLIDDQAGFDASNPALLT
                     PYLCGLYTVITFFEYWSRPQPKKLGPFLVLMACIAYGTALAMLDGRFRSSIVDVLKWT
                     VGPLFAVYLLANRHNIPDLRRIFEPCLIAAAVVMSLYGVWQFVSPSSWDATWVHGVLE
                     LGFNSIGRPEPFQLRVFSTMNSPGSFGAVLSAGVILALKRRLPICLLAVAPMLVGLAL
                     CQYRSLWAATALAIVLVALAPSTGVRRTNILALFFVGLMLASTALSPQIRDAVAKRAA
                     SLTELEGDESLRKRLEQYNEVLQHDNLIVGDGLAINGASRRLDNRHTVEIDGALIEVY
                     RAMGVFGGTAFFLSIAALIAGLFRASPGLGTDIYYDRAIVVALFVQFPIGTVHIGELG
                     FCAWNFLGFGLAARVAAQERA"
     gene            3364..4530
                     /locus_tag="VIV63_10100"
     CDS             3364..4530
                     /locus_tag="VIV63_10100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6124996.1"
                     /translation="MKESRGGSILFITHVGDPGGAEFKMMAIAGTMRDSSEVLLLQHG
                     SLEGLLAAAGIRCSVKPLSADTRKVRREGGFMSLLRAVPGSLSMISFLARKARSFDVV
                     VCFSQKAFVLASLAKPFMRRPILWFMNDILSADHFHPSMIRWLIRLARFSANGVAVVS
                     QESLNVWLRAGGRREGVSVVVSGIDPAQIARQLSDSSRVAGYRQRFAPDGKPLIGMFG
                     RICRWKGQDVFLRALALVPDARGVIAGGALFEEQAFERELHELAAQLGVSDRVTFAGH
                     VDDVMTLMAACDVVAHCSTAPEPSGRVIAEAMFAGTPVIGSDAGGVPEFISHGETGQL
                     TPLNDHEALAAAMRRYLENPQWSREVAARARERAEENYSSRATIRGFHRAMAGL"
     gene            4536..>4817
                     /locus_tag="VIV63_10105"
     CDS             4536..>4817
                     /locus_tag="VIV63_10105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6124997.1"
                     /translation="MLQPAGSRWRRLGAQLGVSDRVTFAGHVDDVMTLMAACDVVAHC
                     STAPEPSGRVIAEAMFAGTPVIGSDAGGVPEFISHGETGQLTPLNDHEAL"
BASE COUNT          794 a         1539 c         1592 g          892 t
ORIGIN      
        1 gatagtgttc gttcatggcg accgggtgcg gacgcgggcc gacgatgctc atgctgcctt
       61 cgagcacatt gaagagctgc ggcagttcgt cgatggacgt gcggcggatg agcgcgccga
      121 tacgtgtaat gcgcgaatcg ccgcgtgcgg cggccttgaa ggtctgtttg ccgtcctcgg
      181 tgacggtcat cgagcggaac ttgagaacca ggatttcgcg gccatcgagg ccgtaccgct
      241 tctggcggaa gaacacgggg cccggggaat cgagccggat cgcgacggcc acggcgagca
      301 gcaatggcgt cagcagcagc accgcggacg cggcgatggc gatgtcgctc aggcgcttga
      361 cgagccaggc cgcgccataa aacggcgttt cacggaccgc gaccaccggc acgccgccca
      421 ggatgtcgaa acgcgcctgg atgagattga acgcgaagat gtccggcacg aagtagatcg
      481 aagcggtgga atcgtgcagc gcctcgagca tcgcgacgat gcgcgcgtcg cggctcatgg
      541 gcagcgtgat gaacacctgg tcgatctggt tgtgcgtgac gtactccgcg aggccgttgg
      601 tgtcgccgag cagcggtgcg gtgaaattgg ccgtgagata ggggcggggc aggtcgtcga
      661 agtagcccac gatgtccgtg cgcagcagcg aatccgcacg cagcgcgcct tcgaggcgtg
      721 caccgagttc ggtggcaccg acgatcacgg cgcgctgcgg ccggcggccc cgcaacgaat
      781 gcgtgagcca ctggcgcgcc gcgaactggc cgacgaacag gaccgccggt gtgaacacga
      841 accagttgat gagcagcgcg aggtcgagcg tgtgcgccac gccggcgaga tagaccaccg
      901 ccgcgatgag cgcgaccagc gtgatccagc gcgcggtgat ctccagcagc agcgcgcctt
      961 gcgggcgttg ttcgtcgctg gcgctgatgc gcgcgaggcc gagtacgtcg ggggcgccga
     1021 ggaatgtcag cacggcgatg aggaaatagc cgccgcgcag ctgctcgttc gcggccgcca
     1081 tgcagatcca cagcgtgatg accggaatga tggggagcag aaggctcttg accgcgaaca
     1141 ccaccggtga atcgacggtg gaaagcgcgg tgacttctct tgaatgcagg ggctcgattc
     1201 gaccttccat cagctaaacc acatcatctg aactgccggg gcattcgcat cgacttctga
     1261 cgtcgtctga gttgcaacgc tatcagcgcg gcggccagca ccacggcgag agcccggtcc
     1321 gagctgccgt cgctgggcgc aggcgcgccg cgcacgagca cgggcgcgta catcgacacg
     1381 ttcggttcgg cgccggccga aggcgcggtt ctcgcggtga acgaggcggc cagggacaga
     1441 aacaagatag ccaggtattt catatgcact ccttgcaggg aaagcagcgg caagcgtcac
     1501 tgctttgcga tgcaagtccc gttccatgat cacccgcgat ggcagtgcgc tggcgtcgca
     1561 cgtcatggct ccggtggatc gatatcagca cttgcaacgc gaaggtgcac cgcccgggcg
     1621 cacccgtcaa gtgcatacgc tcatgccaaa gtagttagac ctgtcggcaa tcggcgaatg
     1681 acgcagtgca gatcggggtt tgtaggtgag aaaatttccg cgatgcatga agagagatca
     1741 tgctcgcgca ctgaaaaaca agcctccgca gcgcaagtga ctctatgcag cgacgtcgga
     1801 caaatcccac gacgcactga tggtgcgcaa aaaatgcagt cgtcgcactt aaatagatag
     1861 acactccgtg tttatacgca gtgcgtgcgc ccgcagtgcg ggttcatgca gaatcgaatt
     1921 ggaatggcac ttgcaactct cgaagcagct gatggaacag acactcccga cccccgcaac
     1981 tacgaaggca agcgcctcat cgccgctcat cgccgaaggg ttggccgttg tgttcgcggt
     2041 gctcgtgctg ccggtgttcc tctacgtcgc gcatctcgcg ccgctcggcc gcctcctgta
     2101 cccggtcgcg aacttcctgc tcgccggata tctcttctcg cgccgctcgc cgtggtacgc
     2161 gtcgcactgc ctgctcgtgt tctgcttcat gtcgctggcg cgccgtctca tcgacgacca
     2221 ggcgggcttc gacgcttcga acccggcgct gctcacgccg tacctgtgcg gcctctacac
     2281 cgtcatcacg ttcttcgagt actggtcgcg gccccagccg aagaagctcg gccccttcct
     2341 cgtgctcatg gcctgcatcg cctacggcac cgcgctggcg atgctcgacg gccggttccg
     2401 ctcgtcgatc gtcgacgtgc tcaagtggac ggtcggcccg ctgttcgcgg tctatctact
     2461 cgcgaatcgc cacaacattc ccgacctgcg gcgcatcttc gagccctgcc tgatcgccgc
     2521 cgcggtcgtc atgtcgctgt acggcgtctg gcagttcgtg agtccgtcct cgtgggatgc
     2581 cacctgggtt cacggggttc tcgagctggg cttcaattcc atcggccggc ccgagccgtt
     2641 ccagctgcgc gtgttcagca ccatgaactc gccgggctca ttcggcgccg ttctgtcggc
     2701 gggcgtcatc ctcgcgctca agcggcgcct gcccatatgc ctgctggcgg tggcgcccat
     2761 gctcgtgggc ctggcgttgt gtcagtaccg ttccctgtgg gccgccaccg cgctggccat
     2821 cgtgctcgtg gcgctggcac cgagcacggg cgtccgccgc acgaacattc tcgcgctgtt
     2881 cttcgtgggc ctcatgctgg catccaccgc gttgtcgcct cagatccgcg acgccgtggc
     2941 gaagcgggcc gcctcgctga ctgagctcga aggcgacgag tcgctgcgca agcggctcga
     3001 gcagtacaac gaagtgcttc agcacgacaa cctcatcgta ggcgacgggc ttgccatcaa
     3061 cggcgcatcg cgccgcctcg acaaccggca caccgtcgag atcgatggcg ccctcatcga
     3121 ggtttatcgc gccatgggcg tgttcggcgg gacggcgttt ttcctgtcca tcgccgcgct
     3181 catcgcgggg ttgtttcgcg catcgcccgg gctgggtacc gacatctact acgaccgggc
     3241 catcgtggtc gcattgttcg tgcagtttcc catcggaacc gttcacatcg gtgagctcgg
     3301 attctgcgcg tggaattttc tgggttttgg tctggccgcg cgcgtggccg cgcaggagcg
     3361 cgcatgaaag agtcccgtgg cggttcgatc ctcttcatca cgcacgtggg tgatccgggc
     3421 ggcgccgaat tcaagatgat ggcgatcgcc ggcaccatgc gcgactcgtc tgaagtgttg
     3481 ttgttgcagc atggatcgct cgaaggactg ctcgccgccg cgggaatccg ctgctcggtg
     3541 aagccattgt ccgccgacac gcgcaaggtg cgccgtgaag gcggcttcat gagtctgctg
     3601 cgagcggttc ccggttcgct gtcgatgatc tcgttcctcg cccgcaaggc gcgctcgttc
     3661 gacgtcgtcg tgtgtttctc gcagaaggcc ttcgtgctcg catcgctggc gaaaccgttc
     3721 atgcgccgtc ccattctctg gttcatgaac gacatcctgt ccgcggacca tttccatcct
     3781 tcgatgatcc gctggctgat ccggctggcg cgtttcagcg ccaacggtgt ggccgtggtc
     3841 tcgcaggaaa gtctcaacgt gtggctgcgc gccggtgggc gccgcgaagg tgtcagcgtg
     3901 gtggtgtccg gaatcgaccc cgcgcagatc gcacggcagc tcagcgattc atcgcgcgtc
     3961 gcggggtatc ggcaacgttt cgcgcccgat ggcaagccac tcatcggcat gttcggccgc
     4021 atctgccgct ggaagggcca ggacgtcttc ctgcgcgcgt tggcgctggt gccggatgcg
     4081 cgtggcgtga tcgcgggcgg cgcgctgttc gaagagcagg ccttcgaacg tgaactgcac
     4141 gaactcgccg cgcagctggg cgtgagcgac cgcgtcacgt tcgcggggca tgtcgacgat
     4201 gtcatgacgc tcatggcggc ctgtgatgtc gtggcgcatt gctcgacggc gcccgaaccc
     4261 tccggccgcg tgatcgccga agccatgttt gccggcacgc ccgtcattgg cagcgatgcc
     4321 ggcggcgtgc cggagttcat cagccacggt gaaaccgggc agctcacgcc tctcaacgac
     4381 catgaagcgc tggccgccgc gatgcgccgt tatctcgaaa atccgcagtg gtcgcgcgaa
     4441 gtcgcggcgc gggcgcgcga gcgcgcggag gagaactact cctcgcgcgc gaccattcgc
     4501 ggctttcacc gcgcgatggc aggcctttga gcagcgtgct ccaaccggcg ggctcacggt
     4561 ggcgccgcct cggcgcgcag ctgggcgtga gcgatcgcgt cacgtttgcc gggcatgtcg
     4621 acgatgtcat gacgctcatg gcggcctgtg atgtcgtggc acattgctcc acggcgcccg
     4681 aaccgtccgg ccgcgtgatc gccgaagcga tgtttgcggg tactccggtc atcggcagcg
     4741 atgccggcgg cgtgcccgag ttcatcagcc acggtgaaac cgggcagctg acgccgctca
     4801 acgatcacga ggcgctc
//
LOCUS       DATDEU010000423        10995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1246936, whole genome shotgun sequence.
ACCESSION   DATDEU010000423 DATDEU010000000
VERSION     DATDEU010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246936"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VIV63_10110"
     CDS             complement(<1..1262)
                     /locus_tag="VIV63_10110"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEY6124998.1"
                     /translation="MSKLFKRTSLGATGLAIVGVLFIAIMLLANLLLRGAKFDLTSDN
                     LYTISEGTDHIVQGLGEPVNLYLFFSEKTATPIPQLRNHGIRVRELLEELVSRSNGKL
                     TLKVIDPQPFTEEEDRATELGVQAVPISAAGDKLYLGLAATNSTDGKEAIAFLDPQSE
                     EQLEYDVAKLIHKLSNAKKPVIGWLSSLPMQGDFDMQTGRPRAPWVVYGQVEQLYTVR
                     NLEPSLTAIDKDVDVLVIVHPKELPPAALYAIDQFALRGGRILAFVDPNAQADQAGAD
                     PNNPMAAMMADKSSHLEPLLTAWGLEFKPDQVVADLERGLTVSMREGEAPSQHIAILG
                     FDHGSMAKDVVTAGLDTINMATAGSLKPLDQSKVAGSKLQLEPLIRTSAQAGLLPAQR
                     LNMMSDPGSLRDGFKPEGELIVAARVSGT"
     gene            complement(1275..2009)
                     /locus_tag="VIV63_10115"
     CDS             complement(1275..2009)
                     /locus_tag="VIV63_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6124999.1"
                     /translation="MKTVAIILRRELASYFATPIAYVVLVIFLLLANLFAFSFGALFE
                     RGQADLAPFFTFLPWLYLFLVPAVSMRLWAEERRNGSIELLLTQPITLWQAVLGKFLA
                     AWVFVGLALALTFPIWITVNYLGNPDNGAIFAAYVGSLFVAGGFLAVGSFTSTLTRNQ
                     LIAFLLGMFICFLLLIAGYPATTDWFQTWAPQWVVQGIAALSFLTHFENIVKGVLDLR
                     DVLYYALVAIFFLLASTVVLDARKSK"
     gene            complement(2033..2743)
                     /locus_tag="VIV63_10120"
     CDS             complement(2033..2743)
                     /locus_tag="VIV63_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6125000.1"
                     /translation="MIKTQNLTKNYDGLLAVDDLTFSVEPGEVLGFLGPNGAGKSTTM
                     RMLAGFITPTSGTGSICGHDISSDPLAAKAQLGYLPEGAPHYGEMTVGGFLDFIADLR
                     RLEGAHRKSRLDYVLGRLQLEPVIAQTIDTLSKGFKRRVGLAQAIVHDPKVLILDEPT
                     DGLDPLQKHEVRTLISEIAREKTIVISTHILEEVDAVCTRAIIVARGKLVADDTPAGL
                     KGRAPSGRLEDVFRTVAA"
     gene            complement(2849..3646)
                     /locus_tag="VIV63_10125"
     CDS             complement(2849..3646)
                     /locus_tag="VIV63_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125001.1"
                     /translation="MANDNVPGEQAGQLEALLERWQISLDLHARYAALDEARYWHVQP
                     WPKHERPQRWIIQLARKRILALKRVVAQRQSEGDRAFIEGIEIMGFLATLVGLTSVER
                     FIPLATHETERRDVLSAKPESVKPSPTPAAAKVVPVPVETVRSRTGEHKRTAETTRQM
                     PALARRDAKRDPARPATVKPQPVARAAPSVKPQPVVRPRKGPANVTAMEHVVIADAVR
                     LLGWGKQWHELAEMIARLSERPSPSEIRRILRTYRDQIDAVASRRRD"
     gene            3792..6140
                     /locus_tag="VIV63_10130"
     CDS             3792..6140
                     /locus_tag="VIV63_10130"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEY6125002.1"
                     /translation="MQMGLRHTLGALALLVFVASAQAREIRVAVVTDGDRGRSIFSAE
                     AIESELASVAAPDTEIVLPTSKRFAGDWSLEGASAALDRALADRDVDVVLTLGILTSQ
                     QAARRKTLSKPVIAPLVIDPVLQGYPLVEGRSGRHNFTYVADFQSVANEVRAFHEFVN
                     FKYVIALVDDSLMSGLPELSAKATELAAALNVRIGVVRVGGDVDAALASIPAGVDAVY
                     VTPLRFNDAQVHELAQGLQARKIPTFSVVGRSEVEAGLLMTTGGAERDKGRLARRVAI
                     MIQRISQGEDPAVFDVGFPTSQRLLINMQVARAIGFSPRWQFLADAEQVNADTGDAQP
                     LTLLEAMRTALDANPSLAASRERLGSALDDVRIARSELLPSLSASAARTRIDEDRASP
                     LTQAEDTTSAGLSFGQVIYSERAWAGYSIAKSLGAAQEQSQRTDMLDTLTAAATAYLN
                     VLRAKSVEDVRRRNVENTRRNLEISRVREEVGLSGRSDYLRWVSQLARDKQTLLAAEA
                     NRKQAETDMLRILHRPASQPFSTVESGLDDPLTLVSSPRTQAFLDTPAKWAVFMEYAV
                     ATALQNAPEIQQADSILTARQRALSSAGRAFYLPDLALVSSGAKYTDKSGAGSLSVPG
                     APDDESWSVTLQATLPLLTGGERGALRSQARHEVRASEADKAATIDGIEARTRLVLHQ
                     TASSWPAIDLSRQALAAADENLANVTDAYARGAVSVTDLIDAQETSLISGLSATDAKY
                     GFMTDFVNVLRSMGEFEILLDPASREAWYARIEAWFRQNPPK"
     gene            6183..7274
                     /locus_tag="VIV63_10135"
     CDS             6183..7274
                     /locus_tag="VIV63_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6125003.1"
                     /translation="MAFNNNRPGSAALLLIALAGVAGCGAKKEAEPLVRPVRSEIVAG
                     GTTTDSATYTAEIRSRYETDLSFQVAGKLVNRAVDVGATVKKGQLLAQLDQTDQQLGV
                     DAARGAVPAAQSDLARSRSEEARFRDLLERGLTTRAQYQAQQTAVRSAEAKLSQATAE
                     LQLAQQRLGYTTLRAGDDGVVTRVMVEVGTVVSAGQRAFSIARPSELEAVFDVPDGRI
                     GEVKAGSSVLINPLSGESTAWAGRVREISPSADPVTRTYQVRTTIVKPPDTLRLGMTV
                     SVTLARVGGAPNISLPSTAIFQKDGKPAVWIVTAGSTVELRAVTVERYDTDRAFVADG
                     IRTGERVVTAGVHRLSQGEKVKLAAEKPQ"
     gene            7271..10351
                     /locus_tag="VIV63_10140"
     CDS             7271..10351
                     /locus_tag="VIV63_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6125004.1"
                     /translation="MSGESAPAEHGRFNLSAWALTHKPLIGFLMVIALFAGLRAYQDL
                     GRDEDPPFTIKVMIVRAFWPGADANQTAKQLTDRLEKPLESLEFVDFVTSYTKPGEAT
                     IMVNLRDNTPPSAVPGQWYQVRKKLGDIRGQLPQGAIGPFFNDDFGDVYGVIYALTSD
                     GFTYRELRDQAEFIRAELLRVNNVGKVDLIGVQDEVIYIDFSLRQMAGLGIDPDLVAA
                     TLAAQNAVIASGTIETPGERIAVRVSGALDSVEALEGLAIRVGERRVRLADFARVTRG
                     YKDPPSPLYRYNGTPAIGIGVSMSKGGNVLDLGVALEKELARIESDLPVGIDVHRVAN
                     QPEVVDESVGQFTRGLFEAIGIVLIVSFVSLGARAGLVVAISIPLVLAITFVFMEIFE
                     ISLQRISLGALIISLGLLVDDAMIAVEMMVKKMEEGWDKFRAATFAYTSTAFPMLTGT
                     LVSVAGFLPVGFAQSSAGEYCFTLFAVVTIALLVSWVVAVIFTPYIGVAVLKELPPGH
                     VHEHVDTPMSARFRKVLIKSLANRGWVILGTAGAFVVAVVLFGFVQQQFFPSSSRSEL
                     MIDLRLAQNASIKATEAQVHRFEKVLLADPDIVYHSFYVGSGAVRFYLPLNQQLENAN
                     FAQAVVITKSFEVRDAVRARLEKVLAEDFDTLMARVEPLALGPPVDWPLQYRVSGPDV
                     SAVRAIAETVAATMRANANTRVVNFDWNEMTKRIRIDVDQEKARQLGVTSQQVSRALA
                     GALSGRSVTEFRDDIYLIDVQGRAQAQDRGDIARLRDLEVGIASGSSVPLTQIATFEY
                     GLEETVIWRRNRLPTITVQSKVAQGLTAATVTEQLQKPIEALMAKLPPGYRIEIGGAV
                     GESAKGSASIMAVIPVMFILMLFILMLQLHSTQKLLLVMLTAPLAIIGVAIALLISNR
                     PFGFVAMLGVFALVGMIIRNSVVLMAQIKENEDAGMARDLAIVDATMHRLRPILLTAA
                     AAILGLIPIVSEVFWGPMAIAMMGGLFVATVLTLIFLPALYASWYRVKRPEVAA"
     gene            complement(10281..>10995)
                     /locus_tag="VIV63_10145"
     CDS             complement(10281..>10995)
                     /locus_tag="VIV63_10145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6125005.1"
                     /translation="ESFPQAPSATNFVIPNGFDAVAAAGAPPADGALKIVHAGEIYGS
                     RSLVPLLRAVQDLSVRRPERAIHVTTYGALPPAEWQRIRDAGLELFIDVQPRIPFAAL
                     FAELQRAHVLLAVVSDHMTYSTPYKIYDYMAAGRPILGLAPHDAALHELLADSGAGQC
                     VDPTDIDGIGQALEKILFGDGAITRARIDRFRWSNLARDYVNAIDAVSETPAATFTPQ
                     PPAVSRDTSSHTAPAGKSA"
BASE COUNT         1860 a         3447 c         3802 g         1886 t
ORIGIN      
        1 gttcccgaaa cccgcgcggc cacgatgagc tcgccttccg gcttgaagcc gtcgcgcagg
       61 gatcccgggt cgctcatcat gttcagacgc tgcgccggca gcagcccggc ctgcgcgctg
      121 gtgcggatga gcggctcgag ctgcagtttg ctgccggcca ccttcgactg gtcgagcggc
      181 ttcaaactac ccgcggtcgc catgttgatg gtgtccagcc ccgcggtgac gacgtccttg
      241 gccatgctgc cgtggtcgaa gccgaggatg gcgatgtgct gcgaaggagc ttcaccttcg
      301 cgcatggaca cggtgagtcc gcgctcgagg tccgcgacca cctggtcggg cttgaactcg
      361 aggccccatg ccgtgagcag cggttcgaga tgcgaggact tgtcggccat catggccgcc
      421 atcggattgt tgggatcggc gccggcctgg tcggcctgcg cattcggatc gacgaacgcg
      481 aggatgcggc cgccccgcag cgcgaactgg tcgatggcat acaacgcggc gggcggcagt
      541 tctttcgggt gcacgatgac cagcacgtcg acgtctttgt cgatggccgt cagcgaaggt
      601 tcgagattgc gcacggtgta gagctgctcg acctgcccgt agaccaccca cggtgcacgc
      661 ggccggccgg tctgcatgtc gaagtcgccc tgcatgggca gcgaggacag ccagccgatg
      721 acgggcttct tcgcgttcga gagcttgtgg atcagcttgg cgacgtcgta ttcgagttgt
      781 tcttcggact gcgggtcgag gaaagcaatc gcttccttgc cgtccgtgga gttcgtggcg
      841 gcgaggccga gatagagctt gtcgcccgcg gcgctgatcg gcaccgcctg gacgccgagt
      901 tcggtggcgc gatcttcttc ttcggtgaac ggctgtgggt cgatgacctt gagcgtgagt
      961 tttccgttcg agcgcgagac cagttcttcg agcagctcgc gtacgcggat gccgtggttg
     1021 cgaagctgcg gaatgggcgt ggcggtcttc tcggagaaga atagatagag gttcaccggc
     1081 tcgccgaggc cctgcacgat gtggtcggtg ccttcggaga tggtgtagag attgtcggag
     1141 gtgaggtcga acttcgcgcc gcgcagcagc agattcgcca gcagcatgat ggcgatgaac
     1201 aacacgccga cgatcgcgag gccggtggcg cccagggagg ttctcttgaa taactttgac
     1261 atcgtggcga tccgttattt cgacttgcgc gcgtcgagca ccacggtgct cgcgagcagg
     1321 aagaagatgg cgaccagcgc gtaatagagg acgtcgcgca gatccagcac gcctttgacg
     1381 atgttctcga agtgcgtgag gaacgacagc gcggcgatgc cttgcaccac ccactgcggc
     1441 gcccaggtct ggaaccagtc ggtggtcgcg ggatatccgg cgatcagcag caggaagcag
     1501 atgaacatgc cgagcaggaa tgcgatgagc tggttgcgcg tgagcgtgga ggtgaacgag
     1561 cccaccgcga gaaagccgcc ggccacgaac aacgaaccga cgtaggccgc gaagatggcg
     1621 ccgttgtcgg gattgccgag gtagttgacg gtgatccaga tggggaaggt gagggcgagc
     1681 gccagcccca cgaacaccca ggccgcgagg aatttgccga ggaccgcctg ccacagcgtg
     1741 atgggctgcg tgagcagcag ttcgatgctg ccgttgcgcc gctcttcggc ccagagccgc
     1801 atggagacgg ccgggaccag gaatagatag agccagggga ggaacgtgaa gaagggcgcg
     1861 aggtccgcct ggccgcgctc gaacagtgcg ccgaacgaga aggcgaacag gttggcgagc
     1921 agcaggaaga tgacgaggac gacgtacgcg atgggcgtcg cgaaataact cgcaagctcg
     1981 cggcgcagga tgatggctac ggttttcaag gcagctccgg gaaacgtgaa ggtcaggcgg
     2041 cgacggtgcg gaacacgtct tcgagccggc ccgagggcgc gcggcccttc aggcccgcgg
     2101 gcgtgtcgtc ggccacgagc ttgccgcgcg cgacgataat ggcgcgggtg cagacggcgt
     2161 cgacttcttc gaggatgtgc gtggagatga cgatggtctt ctcgcgcgcg atctcgctga
     2221 tgagcgtgcg gacttcgtgc ttctgcagcg gatcgaggcc gtcggtgggc tcgtcgagga
     2281 tcaggacctt ggggtcatgg acgatcgcct gggcgagacc gacacggcgc ttgaagccct
     2341 tggagagcgt gtcgatggtc tgggcgatga ccggttcgag ctgcagccgg ccgagcacgt
     2401 agtcgagccg cgatttgcgg tgcgcgcctt cgagccgccg caggtcggcg atgaagtcga
     2461 ggaagccacc gacggtcatc tcgccgtaat gcggggcacc ctcgggtaga tagccgagct
     2521 gcgccttggc ggccagcgga tcggacgaga tgtcgtggcc gcagatgctg ccggtgccgc
     2581 tggtcggggt gatgaagccc gcgagcatgc gcatggtggt ggatttgccg gcgccgttgg
     2641 ggccgaggaa accgaggact tcgcccggct ccacgctgaa tgtgaggtcg tcgacggcga
     2701 gcaggccgtc gtagttcttg gtcaggttct gcgtcttgat catggcgcct gttcagagcg
     2761 gtcagccgtg gggaagttcc acggctttcg gggcgcagat tctgctcagg agagcccctt
     2821 cgcgcaaggc gtcgggcagc cgccggattc agtcgcgtcg gcggctggcg acggcgtcga
     2881 tctggtcgcg gtaggtacgc aggatgcggc ggatttccga ggggctgggc ctttcggaga
     2941 gccgcgcgat catctccgcg agttcgtgcc actgcttgcc ccagcctaac aaacgtaccg
     3001 cgtcggcgat caccacgtgt tccatcgccg tgacgttggc ggggcctttc ctgggtctga
     3061 cgacaggctg gggtttgacg gagggcgcgg ccctggccac gggctgaggt ttgacggtcg
     3121 ccgggcgtgc cggatcgcgc ttcgcatcgc gccgggccag cgccggcatc tggcgcgtgg
     3181 tctcggccgt gcgcttgtgt tcgccggttc gcgagcgcac ggtttcgacc ggcaccggaa
     3241 ccaccttggc cgccgcgggg gtcggcgagg gcttcacgga ttccggcttc gcgctcagaa
     3301 cgtcccgcct ctcggtttca tgcgtggcca gcggaatgaa tcgttccacc gaggtgaggc
     3361 ccacgagcgt cgcgaggaag cccatgatct cgataccttc gatgaaggcc cggtcacctt
     3421 cggactggcg ctgcgcgacg acgcgcttga gcgcgaggat gcgtttgcgg gcgagctgga
     3481 tgatccagcg ttgcggccgt tcgtgtttgg gccatggctg cacgtgccag taccgcgcct
     3541 catcgagcgc cgcatatcgg gcgtgaagat cgaggctgat ctgccagcgt tcgagcagcg
     3601 cttcgagctg gcctgcctgt tcgccgggta cgttgtcgtt agccatgcac cgaagagtag
     3661 cgctgtgcct gcggaaggtg cgcgatgtgg cgcacatctg tcctgcggat caggctcttt
     3721 tgtgcacgac agcacagttg agcccggact aagatgccga caaacaagaa gccggtcggg
     3781 agaagtcgcg gatgcagatg ggtttgcggc acacactggg cgcgttggcg ttgctcgttt
     3841 tcgtcgcatc ggcgcaggca cgcgaaatcc gcgtcgccgt ggtcacggac ggagatcgcg
     3901 ggcggtccat tttctcggcc gaagccatcg agagcgaact ggccagcgtc gccgcacccg
     3961 acacggagat cgtgctgccc acctcgaaac gcttcgcggg cgactggtcg ctcgagggcg
     4021 cctcggccgc gctcgatcgc gcgctcgcgg accgcgacgt cgacgtggtc ctcacgctcg
     4081 gaatcctcac gtcgcagcag gcggcacgcc gcaagacact gtccaaaccg gtgatcgcgc
     4141 cgctggtcat cgacccggtg ctgcagggct atccactcgt ggaaggccgc agcggacgcc
     4201 acaacttcac gtacgtcgcc gacttccaga gcgtggccaa cgaagtccgc gcattccacg
     4261 agttcgtgaa cttcaagtac gtgatcgcgc tggtcgacga ttcactgatg tccggtctgc
     4321 ccgagctgtc cgccaaggcg acggaactcg ccgcggcgct caacgtgcgc atcggcgtgg
     4381 tccgcgtcgg cggcgacgtc gatgccgcgc tcgccagcat tcccgcggga gtcgacgccg
     4441 tgtacgtgac gccgctgcga ttcaacgacg cgcaggtgca cgaactcgcg cagggcctgc
     4501 aggcccgaaa gattccgacg ttctcggtgg tcggccgcag cgaggtcgag gcgggtctgt
     4561 tgatgaccac cggcggcgcc gagcgcgaca agggccggct ggcgcgtcgc gtggccatca
     4621 tgatccagcg catttcgcag ggcgaggacc ccgcggtgtt cgacgtgggt ttccccactt
     4681 cgcaacggct gctcatcaac atgcaggtgg cgcgcgcgat cggtttctcg ccgcgctggc
     4741 agttcctggc cgatgcggag caggtgaacg ccgacaccgg cgatgcgcag ccgctcacgc
     4801 tactggaggc catgcgtacc gcgctcgatg cgaatccgtc gctggccgcc agtcgcgaac
     4861 ggctcggcag cgcgctcgac gacgtacgca tcgcgcgcag cgagttgctg ccgtcgctgt
     4921 cggcatccgc cgcgcgcacg cgcatcgatg aagatagggc cagcccgctt acgcaggccg
     4981 aggacaccac cagcgccgga ctgtcgttcg gtcaggtcat ctattccgag cgcgcctggg
     5041 cgggctattc catcgccaag tcgctgggcg cggcgcagga acagagccag cgcaccgaca
     5101 tgctggacac cctcacggcg gccgcgaccg cgtacctgaa tgtattgcgc gcgaagtcgg
     5161 tggaagacgt ccgccggcgc aacgtggaga acacgcgccg gaatctcgag atctcgcgcg
     5221 tgcgtgagga agtgggtctg tcggggcgct ctgactacct gcgctgggtg tcgcagctgg
     5281 cgcgcgacaa acaaacgctg ctggccgcgg aagcgaaccg caagcaggcc gagaccgaca
     5341 tgctgcgcat cctgcatcgg ccggcgagcc agccgttcag cacggtggag tcggggctcg
     5401 acgatcccct gacgctggtg tccagtccgc gcacgcaggc cttcctcgat acgcccgcga
     5461 agtgggcggt gttcatggag tacgccgtgg ccacggcgct gcagaacgcg ccggagatcc
     5521 agcaggccga ttccatactg accgccaggc aacgcgcctt gagttcggcc ggacgcgcgt
     5581 tctatctgcc ggacctcgcg ctggtgtcga gcggcgcgaa atacaccgac aagagcggcg
     5641 ccggttcgct gagcgtcccc ggcgcacccg acgatgaatc ctggagcgtg acgctgcagg
     5701 caacactgcc gctgctgacg ggtggtgaac gcggtgccct gcgatcgcag gcgcgacacg
     5761 aagtgcgggc cagcgaggcc gacaaggccg caaccatcga cggcatcgaa gcgcgcacgc
     5821 ggctggtgct gcaccagacc gcaagctcat ggccggccat cgacctctcg cgccaggcgc
     5881 tggccgccgc cgacgagaac ctcgccaacg tgaccgatgc ctatgcgcgc ggcgcggtgt
     5941 cggtcaccga tctcatcgac gcgcaggaaa cttcgctcat ctcgggtctg agcgcgaccg
     6001 atgcgaaata cgggttcatg acggacttcg tcaacgtgct gcgctcgatg ggtgagttcg
     6061 aaatcctgct cgatcccgca tcgcgcgaag cgtggtacgc gcgcatcgaa gcctggtttc
     6121 gccagaatcc gccgaaatga gaatccaccg aaatgagaac cgcccgcgga aagaggcttg
     6181 ggatggcatt caacaataat cgcccgggtt cggccgctct cttattaata gcgctggccg
     6241 gcgttgccgg ctgcggcgcg aagaaggaag ccgagccgct ggttcgtccg gtgcgttcgg
     6301 aaatcgtcgc gggcgggacc acgaccgaca gcgcgaccta cacggcggaa atccgctcac
     6361 gctacgagac cgacctcagc ttccaggtgg ccggcaagct ggtgaaccgc gccgtggatg
     6421 tgggcgctac cgtgaagaaa gggcagctcc tcgcgcaact cgaccagacc gaccagcagt
     6481 taggcgtcga cgcggcgcgc ggcgcggtgc cggccgcgca atcggacctg gcgcgttcgc
     6541 gcagcgaaga ggcgcggttc cgcgacctgc tcgagcgcgg tctcacgacg cgcgcgcaat
     6601 accaggcgca acagactgcg gtgcgatcgg cggaggccaa actatcgcag gcgacggcgg
     6661 agttgcagct cgcgcagcag cgcctgggct acaccacgct gcgcgccggc gacgatggtg
     6721 tcgtgacgcg cgtgatggtc gaggtgggta cggtggtcag cgccggccag cgtgcgttca
     6781 gcatcgcgcg tcccagcgaa ctcgaagccg tgttcgatgt gcccgacgga cgcatcggcg
     6841 aagtgaaggc cggctcgtcc gtgttgatca atccgttgag cggcgagagc accgcgtggg
     6901 ccgggcgcgt gcgtgagatc agtcccagtg ccgatccggt gacgcggacg taccaggtgc
     6961 gcaccaccat cgtgaagccg cccgacactt tgcggctcgg catgacggtc agcgtgacgc
     7021 tggcgcgcgt cggcggcgct cccaacatct cgctgccatc cacggcgatc ttccagaaag
     7081 acggcaagcc cgcggtctgg atcgtgaccg ccggcagcac cgtggaactc cgtgccgtca
     7141 ccgtcgaacg ttacgacacc gaccgggcgt tcgtggcgga cggcattcgc acgggtgaac
     7201 gcgtggtgac cgcgggcgtg caccggctat cgcaaggcga gaaagtgaag ctggcggccg
     7261 agaaaccgca atgagtggag agtccgcgcc ggcggagcac ggccgcttca atctttccgc
     7321 gtgggcgctc acccacaagc cgctgatcgg cttcctcatg gtgatcgcgc tgttcgcggg
     7381 tttgcgcgcg taccaggacc tgggccgcga tgaagatccg ccgttcacga tcaaggtcat
     7441 gatcgtgcgc gcgttctggc ccggcgccga cgccaaccag accgccaagc aattgaccga
     7501 ccggctcgag aagccgctcg agtcgctcga attcgtcgac ttcgtcacca gctataccaa
     7561 gcccggcgaa gccacgatca tggtcaacct gcgcgacaac acgccgccgt cggccgtgcc
     7621 cggccagtgg taccaggtgc gcaagaagtt aggcgacatc cgcggccagc tgccgcaggg
     7681 cgccatcggg ccgttcttca acgacgactt cggcgacgtg tacggcgtca tctatgcgct
     7741 gaccagcgat ggcttcacct atcgcgagct gcgcgaccag gccgagttca tccgcgccga
     7801 gctgctgcgc gtcaacaatg tcggcaaggt cgatctcatc ggcgtgcagg acgaagtcat
     7861 ctacatcgat ttctcgttgc ggcagatggc gggactcggc atcgacccgg atctcgtcgc
     7921 cgcgacgctg gccgcacaga acgccgtcat cgcctccggc accatcgaga cgccgggcga
     7981 acggatcgcg gtgcgcgtca gcggtgcgct cgattccgtc gaggcactcg agggcctggc
     8041 catccgcgtc ggcgagcggc gcgtgcgtct cgcggacttc gcgcgcgtga cgcgcggcta
     8101 caaggatccg ccctcaccgc tgtaccgcta caacggcacg ccggccatcg gcattggcgt
     8161 gtccatgtcc aagggtggca acgtgctcga tctcggcgtc gcgctcgaga aggaactcgc
     8221 gcgtatcgag agtgatttgc cggtcggcat cgacgttcat cgcgtcgcca accagccgga
     8281 agtcgtcgat gaatcggtcg ggcaattcac gcgcggattg ttcgaggcca tcggcatcgt
     8341 gctcatcgtg agtttcgtga gcctgggcgc gcgcgccggc ctggtcgtgg cgatcagcat
     8401 tccgctggtg ctcgcgatca ccttcgtgtt catggagatc ttcgagatca gcctgcagcg
     8461 catttcgctg ggcgcgttga tcatctcgct gggcctcctg gtcgacgacg ccatgatcgc
     8521 agtggagatg atggtgaaga agatggagga gggctgggac aaattccgcg ccgccacgtt
     8581 cgcctacacg tccacggctt tcccgatgct caccggcacg ctggtgtccg tggccgggtt
     8641 cctgccggtc ggattcgccc agagcagcgc cggcgaatat tgcttcacgc tgttcgccgt
     8701 ggtgaccatt gccttgctgg tgtcatgggt ggtcgcggtc atcttcacgc cctatatcgg
     8761 cgtcgccgtg ctcaaggaac ttccgccggg ccacgttcat gaacacgtgg acactccgat
     8821 gagcgcgcgt tttcgcaagg tgctcatcaa gtcactggcc aatcgcggct gggtcatcct
     8881 gggcaccgcc ggtgcattcg tggtcgccgt cgtgctgttc ggcttcgtgc agcaacagtt
     8941 ctttccgtcg tcgagccgct ccgagctcat gatcgacctg cggctcgcgc agaacgcctc
     9001 catcaaggcc accgaggcgc aagtgcatcg cttcgagaaa gtgctgctgg ccgatcccga
     9061 catcgtctat cacagcttct acgtcggctc cggcgcggtc cgtttctatc tgccgctcaa
     9121 ccagcagctc gagaatgcga acttcgcgca ggcggtggtg atcacgaaga gcttcgaggt
     9181 gcgcgacgcg gtgcgtgcac ggctcgagaa ggtgctggcc gaagacttcg acacgctgat
     9241 ggcgcgcgtg gagccgctgg cgctcgggcc gccggtcgat tggccgctcc agtaccgcgt
     9301 gagcgggccg gacgtcagtg ccgtgcgcgc gatcgccgaa acggtggccg cgacgatgcg
     9361 cgcgaatgcg aatacgcgcg tggtgaactt cgactggaac gagatgacca agcgcatccg
     9421 catcgatgtc gaccaggaaa aggcgcggca gctcggggtc acttcgcagc aggtgtcgcg
     9481 cgcgctggcc ggcgcgttgt cggggcgctc ggtcaccgag ttccgcgacg acatctatct
     9541 gatcgacgtg cagggccgcg cgcaggcaca ggatcgcggc gacatcgcgc ggctgcggga
     9601 cctggaggtc ggcatcgcca gcggcagctc cgtgccgctc acgcagatcg ccacgttcga
     9661 atacggcctc gaggaaacgg tgatctggcg ccgcaaccgg ttgccgacga tcaccgtgca
     9721 atcgaaggtg gcgcagggtc tgacggccgc caccgtgacc gaacaactgc agaaacccat
     9781 cgaggcgctg atggccaaac taccgccggg ttatcgcatc gagatcggcg gcgcggtggg
     9841 cgaaagcgcc aaggggtcgg catccatcat ggccgtgatc ccggtcatgt tcatcctcat
     9901 gctgttcatc ctgatgctgc agcttcacag cacgcagaaa ctgctgctgg tcatgctgac
     9961 cgcgccactg gccatcatcg gcgtggcgat cgcgctgctg atcagcaacc ggccgttcgg
    10021 attcgtggcg atgctcggcg tgttcgcgct cgtcggcatg atcatccgca attcagtggt
    10081 gctgatggcg cagatcaagg agaacgagga tgcgggcatg gcgcgtgacc tcgcaatcgt
    10141 cgacgccacc atgcaccggt tgcggcccat ccttctgacg gccgcggcgg cgatactcgg
    10201 actcataccc atcgtcagcg aagtgttctg gggccccatg gccatcgcga tgatgggcgg
    10261 cctgttcgtc gcgaccgtgc ttacgctgat tttcctgccg gcgctgtatg cgagctggta
    10321 tcgcgtgaaa cggccggagg ttgcggcgtg aacgtggccg cgggcgtttc gctcaccgcg
    10381 tcgatggcgt tcacatagtc gcgggcgagg ttcgaccagc ggaaacggtc gatacgcgcc
    10441 cgcgtgatcg cgccatcacc gaacaggatt ttctcgagcg cctgcccgat gccgtcgata
    10501 tcggtggggt cgacgcactg gcccgcgccg ctgtcggcta acaactcgtg cagcgctgcg
    10561 tcgtgcggcg ccaggccgag gatgggccgg cccgcggcca tgtaatcgta gatcttgtac
    10621 ggcgtcgaat aggtcatgtg atcgctgacc acggcgagca gcacatgcgc gcgttgcagc
    10681 tccgcgaaca acgcggcgaa tggaatacgc ggctggacat cgatgaatag ttcgaggccg
    10741 gcatcgcgaa tgcgctgcca ttcggcgggc ggcaatgccc cgtacgtggt gacgtgtatc
    10801 gcgcgctcgg gacggcgaac gctcaggtcc tgcactgcgc gcagcagggg caccagcgag
    10861 cggctgccgt agatctcgcc ggcgtgcacg atcttcagcg cgccgtcggc gggtggcgct
    10921 ccggccgcgg ccacggcgtc gaagccgttc ggtatcacga agttcgtggc cgagggcgcc
    10981 tgcggaaaag attcc
//
LOCUS       DATDEU010000424         2349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1539170, whole genome shotgun sequence.
ACCESSION   DATDEU010000424 DATDEU010000000
VERSION     DATDEU010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539170"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VIV63_10150"
     CDS             <1..434
                     /locus_tag="VIV63_10150"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY6125006.1"
                     /translation="VAERNQLVNLLAELRGSGEIGPGEPGSSDQAPNWLPIRNGRETV
                     RVPVQAIEWVDAAGDYLCIHADGHTHILRATMREMENLLDPRLFQRVHRSTIVNLTRV
                     KSLRAHMNGEYFLRLEGGQELKLSRTYRDKVEYFLKRPRSH"
     gene            511..1614
                     /locus_tag="VIV63_10155"
     CDS             511..1614
                     /locus_tag="VIV63_10155"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEY6125007.1"
                     /translation="MKHILSALAVTIASVPLAATAATLHVANNGLDAPGCGTETSPCR
                     SISAAIAAAADDDTVLVRPGRYGDIDDDGALGSAGEETGNYNGAVYINKRVKVISTAG
                     AGATVIKGVSSIPLVVYMDTSGAQFGDRNAGFTVHGANSFGVSNNNMASGKIAGNIAS
                     GLAVGFYIVSIGDVEISYNTAIGNTAAGIIGASSGETNGSTFIHHNTVVGVSNSSGIS
                     VSAIGAHRVVGNTISNNYIGLQVGVGPSRVTQNVITDNFYAIAYPGECIGCATPAGTP
                     LIVRNSLIGSRNAALWVSHLATYPIAMRQNNLFSSGWGCAINTSTTVAIDARQNFWGT
                     ASGPGFTAPSSGVCTTNPVVKTTPFSATEIALN"
     gene            complement(1650..1868)
                     /locus_tag="VIV63_10160"
     CDS             complement(1650..1868)
                     /locus_tag="VIV63_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125008.1"
                     /translation="MARRKAPAEESPADVAQPVAPRNSRTPVIMWIDDSAVCVEIVDI
                     PVSHPRDGVCRCEACHADLADDWASRFL"
     gene            complement(1883..>2349)
                     /locus_tag="VIV63_10165"
     CDS             complement(1883..>2349)
                     /locus_tag="VIV63_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125009.1"
                     /translation="LVNDQDLEIVARAPLTTADTVTAGADVQLIDGDRKATGKVRAII
                     PVGDERSRMVELRITMTDSWRVGAPVRVEIAPRSLRKVVTVPRDAVILRQGASYVMRV
                     RPDNTAERVAVNIGPGQANHVQIDGLLKAGDRVIIRGGERLEPGQTVKVQLL"
BASE COUNT          436 a          803 c          724 g          386 t
ORIGIN      
        1 cggtggcaga gcgcaaccag ctggtgaatc tgctggccga attacgcggc agcggtgaaa
       61 tcggaccggg agaacccggc agcagcgatc aggctccgaa ctggctgccg atccgcaatg
      121 gacgcgagac cgtgcgcgtg cccgtgcagg ccatcgaatg ggtggatgcc gcgggcgatt
      181 acctgtgtat ccacgccgac ggccacacgc acatcctgcg cgccaccatg cgcgagatgg
      241 agaacctgct cgacccccgg ctgttccagc gcgtgcatcg ctcgacgatc gtgaacctca
      301 ctcgcgtgaa gagcctgcgc gcccacatga acggcgaata tttcctgcgg ctcgaaggcg
      361 gccaggagct gaagctgtcg cgcacctatc gcgacaaggt cgaatacttc ctcaagcggc
      421 cgcgctcgca ctgacccgta tcgccacggc gttctagtta ggcatactcc ccggcgccgc
      481 agcgtgcggc cgtgtcacaa gggagtcccg atgaaacata ttctcagcgc gttggcggtc
      541 accatcgcgt ccgtgcccct ggccgccacc gcggccacgc tccacgtcgc caacaacggg
      601 ctcgatgctc ccggatgcgg cacagaaacc tcgccgtgcc ggtccatttc agccgccatc
      661 gcggccgctg ccgatgacga caccgtcctc gtccgccctg gccgttatgg cgacatcgac
      721 gacgacggtg cgctcggcag cgccggcgaa gaaaccggca actacaacgg cgccgtatac
      781 atcaataaac gcgtcaaggt catctcgacg gccggtgccg gcgccaccgt gatcaagggc
      841 gtcagcagca tcccgctcgt cgtatacatg gacacgagcg gcgcgcagtt tggcgatcgc
      901 aatgccggct tcaccgtgca cggcgcgaat tctttcggcg tcagcaacaa caacatggca
      961 agcggcaaga tcgccggcaa catcgcgagc ggcctggccg tcggcttcta catcgtgtcc
     1021 attggcgacg tcgagatcag ctacaacacc gccatcggca acacggcggc ggggatcatc
     1081 ggtgcgtcgt ccggagaaac gaacggcagt acgttcatcc atcacaacac cgtggtgggc
     1141 gtgtcgaact cctcgggtat cagcgtcagt gcgatcggag cgcaccgcgt ggtcggcaac
     1201 accatcagca acaactacat cgggttgcag gtcggagtcg gcccgtcgcg agtgacgcag
     1261 aacgtgatca ccgacaactt ctacgccatc gcctatcccg gtgagtgcat cgggtgcgcg
     1321 actcccgcgg gcacaccgct gatcgtgcgc aactccctca tcggcagccg caatgcggcg
     1381 ctgtgggtga gccacctggc tacctatccg atcgcgatgc gccagaacaa cctgttcagc
     1441 agtggctggg gctgcgcgat caatacgtcc accacggtgg cgatcgacgc gcgtcagaac
     1501 ttctggggaa ccgcatcggg accgggtttc acggctccgt cgagtggtgt ctgcacgacg
     1561 aatccggtgg tgaagaccac cccgttctcg gccaccgaga tcgcgctcaa ttgaaaccgc
     1621 cggggccgcg ggcatctggc ccgcggccct cagaggaagc gcgacgccca gtcgtcggcg
     1681 aggtccgcgt gacaagcctc gcaacggcat acgccgtcac gcggatgact gaccggaatg
     1741 tcgacgatct ccacgcacac cgcggagtcg tctatccaca tgatcaccgg cgtgcgcgag
     1801 ttgcgcggcg caacgggctg cgcaacgtca gcgggtgact cttctgctgg cgccttcctt
     1861 cttgccatgt gacgcatggc gcctacaaaa gctgaacttt cacggtctga cccggctcga
     1921 ggcgttcacc gccgcgaatg atcacgcggt cgccagcctt cagcaggcca tcgatctgca
     1981 cgtgattggc ctgtccgggg ccgatgttca ccgccacgcg ctccgccgtg ttgtcgggcc
     2041 gcacccgcat cacgtagctc gcgccctggc gaaggatcac ggcatcccga ggcacggtga
     2101 cgaccttcct gaggctgcgc ggcgcaattt ccacgcgtac cggcgcgccc acgcgccacg
     2161 aatccgtcat cgtgatgcgc agttcgacca tgcgtgaacg ctcgtcaccc acgggaatga
     2221 tggcgcgcac cttgcccgtg gccttgcgat caccgtcgat gagctggacg tcggcgcctg
     2281 cggtcacggt atcggcggta gtcaggggcg cccgcgccac gatctcgagg tcctgatcgt
     2341 tcaccagac
//
LOCUS       DATDEU010000425         2654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_80016, whole genome shotgun sequence.
ACCESSION   DATDEU010000425 DATDEU010000000
VERSION     DATDEU010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80016"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VIV63_10170"
     CDS             complement(<1..785)
                     /locus_tag="VIV63_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125010.1"
                     /translation="MNATIADARVRTTFLTGPIISGALWDSVKKLNPRVQFRNPVMFV
                     VYVGSILTTLIGIAAAAGAAADAGRPAFVMAVSAWLWLTVLFANFAEAVAEGRGKAQA
                     ATLRSMRKLVIAKRLKGTSRLEHEPVEGSALRKGDLVLVEANDTIPADGEVVEGVASV
                     NESAITGESAPVLREAGGDFGSVTGGTRVLSDWLVVRVSSREGEGFLDRMIAMVEGAS
                     RARTPNEIALAILLAMMTLVFLLATATLAPFSEYAVASSGEGSA"
     gene            complement(804..2597)
                     /gene="kdpA"
                     /locus_tag="VIV63_10175"
     CDS             complement(804..2597)
                     /gene="kdpA"
                     /locus_tag="VIV63_10175"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY6125011.1"
                     /translation="MFQVVVYLGVLLALVKPLGTFMAAVFEGRRTFLSPLIRPLERGI
                     YRLSGVAEEQEAGWKRYALGLLLVNLAGFLTVYLLQRFQGVLPLNPQGFGAVSPDSSF
                     NTAVSFASNTNWQGYVGESTMSNLTQMLGLAVQNFLSAASGVAVLVALIRGFARRQVA
                     EIGNFWVDFTRSTLYILLPLSLVLAIILASQGVVQSFQSYRTVTLIDPVTIETPVTND
                     AGEPVLGADGQPTMRAEQITEQTVPLGPAASQIAIKQLGTNGGGFFNVNSSHPLENPT
                     PLSNFLQVLAILLIPAALCYTFGVMVRDKRQGWAVLIAMLVIFVPLTLAGIAAEQSGN
                     PIIAALDVDQQASDLQSGGNMEGKETRFGITNSGLWASATTAASNGSVNAMHGSFTPL
                     GGLVPMWLMQLGEVVFGGVGSGLYGMLMFAIVAVFIAGLMVGRTPEYLGKKVESFEVK
                     MASLTLLIPPAVVLIGTAIASVLPAGTASVGNPGAHGFSEILYAFSSAGNNNGSAFAS
                     ISANTPFYNTMLGIAMWISRYWLMIPVLAIAGSLAAKRATAVTAGTLPTHTPLFIVIL
                     VGVVLLVGALTFVPALALGPVVEHLQMIGHP"
BASE COUNT          521 a          877 c          830 g          426 t
ORIGIN      
        1 gcggagccct cgccggaact cgcgaccgcg tattcagaga acggcgccag cgtcgcggtg
       61 gcgagcagga acaccagcgt catcatggcg agcaggatgg ccagcgcgat ttcgttcggc
      121 gtgcgggcgc gcgatgcacc ctcgaccatg gcgatcatgc gatcgaggaa accctcgcct
      181 tcgcggctgc tcacgcgcac gacgagccag tcggacaaaa cgcgagtacc gccagtgacc
      241 gagccgaaat caccgcccgc ttcacgcaga accggcgcgg actcacccgt gatggcgctt
      301 tcgttgaccg aagcgacgcc ctccaccact tcaccgtcgg cgggaatcgt gtcgttggcc
      361 tccacgagca ccagatcgcc tttcctgagc gcgctgcctt ccactggctc gtgctccagg
      421 cgtgacgtcc ctttcaatcg cttggcgatc acgagtttgc gcatcgagcg cagcgtggcc
      481 gcctgcgcct tgccacggcc ttcggccacc gcctcggcga agttcgcgaa cagcaccgtg
      541 agccacagcc acgcggacac cgccatcacg aaggccgggc gtcccgcatc ggccgccgcg
      601 cctgcagcgg cggcaattcc gatcagagta gtcaggatgc tgcccacgta caccacgaac
      661 atgaccgggt tgcggaattg aacgcgtggg ttcaatttct tcacgctgtc ccacaacgcg
      721 cccgagatga tcggtccggt cagaaaggtg gtacgcaccc gggcatcggc aatagtcgca
      781 ttcatgtttt caacgctcgc ttgtcaaggg tggccgatca tctgcaggtg ctcgaccacg
      841 gggccgagcg ccaacgcggg tacgaacgtg agcgcgccca ccaacaagac gacacccacc
      901 aggatcacga tgaacagcgg cgtgtgcgtg ggcagcgtgc ccgcggtcac cgccgtggcg
      961 cgtttcgccg ccagactgcc ggcgatggcc agcacgggaa tcatcaacca gtaacgcgag
     1021 atccacatcg cgatgcccag catcgtgttg tagaacggcg tgttcgccga aatgctggcg
     1081 aacgcgctgc cgttgttgtt gcccgccgag gaaaacgcgt agaggatctc cgagaatcca
     1141 tgcgcgccgg gattgccgac gctcgcggtg cccgcgggca ggacgctggc gatcgcggtg
     1201 ccgatgagca ccaccgccgg aggaatcagc aatgtcagtg acgccatctt cacctcgaaa
     1261 ctttcgacct tcttgccgag atactcgggc gtgcgcccga ccatgagacc ggcgatgaac
     1321 accgcgacga tggcgaacat cagcatgccg tagagcccgc tgcccacgcc gccaaacacc
     1381 acttcaccga gctgcatgag ccacatcggc accagcccgc cgagcggggt gaacgaaccg
     1441 tgcatggcgt tcaccgatcc gttcgacgcg gcggtggtgg ccgacgccca cagaccggaa
     1501 ttggtgattc caaaacgcgt ttccttgcct tccatgttgc cgccggattg aaggtccgac
     1561 gcctgctggt ccacatcgag agccgcgatg atcgggttgc cggactgttc cgccgcgata
     1621 cccgccagcg tgagcggcac gaagatcacc agcatggcga tgagcaccgc ccagccctgg
     1681 cgcttgtccc gaaccatgac tccgaacgtg taacagagcg ccgcgggaat cagcaggatc
     1741 gccagcacct gcaggaagtt ggacagcggc gtcgggttct ccaacgggtg cgaggaattc
     1801 acgttgaaga atccgccgcc gttggtgccg agctgtttga tggcgatctg gctcgcggcc
     1861 ggaccgagcg gcacagtctg ttccgtgatc tgttcggcac gcatcgtcgg ctggccgtcg
     1921 gcgccgagca ccggctcacc ggcgtcgttc gtcaccgggg tttcgatcgt caccggatcg
     1981 atcagcgtca ccgtgcgata cgactggaag ctctgcacca caccctgtga ggcgaggatg
     2041 atggccagca ccagtgacag cggcagcagg atgtagagag tgctgcgcgt gaagtcgacc
     2101 cagaagttgc cgatctccgc cacctgccgg cgcgcgaatc cgcggatgag cgcgaccagg
     2161 acggccacgc cggaggcggc cgacagaaag ttctgcacgg ccaggcccag catctgtgtg
     2221 aggttactca tcgtcgactc gccgacatag ccctgccagt tggtgttgct ggcgaaactc
     2281 accgcggtgt tgaacgagga atccgggctg accgcgccga agccctgcgg attgagcggc
     2341 aacactccct ggaagcgctg caatagatag acggtgagga aacccgccag gttcacgagc
     2401 agcaggccga gcgcgtagcg tttccagccg gcctcctgct cctccgcgac accggaaagg
     2461 cgatagatgc cgcgttcgag cggcctgatg agcggcgaca gaaacgtgcg ccggccttcg
     2521 aaaactgccg ccatgaacgt gcccagcggt ttcaccagcg ccagcaggac gcccaggtag
     2581 acgacgactt ggaacaatcc ttgtccggtc acttgaattt ctccgggtat aacagcgcga
     2641 ccacgaggta cacg
//
LOCUS       DATDEU010000426         6993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_664292, whole genome shotgun sequence.
ACCESSION   DATDEU010000426 DATDEU010000000
VERSION     DATDEU010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664292"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VIV63_10180"
     CDS             <1..496
                     /locus_tag="VIV63_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125012.1"
                     /translation="LSSLRRQWASMPAHAVRRLSAAPFSLFDAGFASAPRWLEVRTRG
                     VHELPQRVEPAFFPPEMARSITRRMLVYGWHLARSRPRVACLVFGSTMAGIESLAVCS
                     LASLEAAADRNAESLRPRWSSQTHFWRELLLAASAGADRRLHELLLGGVQRLAAETLA
                     AHSP"
     gene            598..2583
                     /locus_tag="VIV63_10185"
     CDS             598..2583
                     /locus_tag="VIV63_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125013.1"
                     /translation="MTNNRSAIAAIAAILLSISSGASAASPDDAIAARNAAKSGEGAR
                     LGASLAVLQKAAARRVPQPDVTRRVTRKLPALRASQGYVSVSAYGDDLPALRTQLESR
                     GLIDAVVHPTAVSGKAPVAALADMARTSGLKFLRPTLAMARASRGAIVSQGDRSLRAD
                     RARRDSGATGRGIRVGVLSDSYDCAPGPFEPGSPFTRAAEDIANGDLPRDVLVLKDLA
                     SAPSGDCSDEGRAMMQLVHDVAPGSPLAFYTAFESQEDFAAGIRALAAAGSKVIVDDI
                     IYFAEPMFEDGIIAQAVDDVHAAGVAYFSSAGNDARLSYESRYRQSAEPGISGPRHDF
                     APGGRVDGLQTATASAGSATLLSVQWDQASLSANGRRGAKSDVDVWFYDMNGDPIEIC
                     TDDPAQLICQIPGFDANIDGDAVETPIMVNFSDDDLEVQIGIELFEGPVPNYVKYVWF
                     DLDAGGFTVDEYDTASGTVYGHANAAGAEAVGAAAWYQTEEWGSPLRPQCIPACLNSF
                     SSAGGTPLFFGPNGRRLSAPLVRLKPGITGPDGGNTSSFFFDLGFEIPGTSEPDGIPN
                     FFGTSASAPHVAGVAALMLDQRARDVAARKRFIGPRTLTPDLIYWAMRLTADDMKLRN
                     FGGDIGPQRVDNANGYDFDTGFGFVDAQRALRATRGF"
     gene            2700..3362
                     /locus_tag="VIV63_10190"
     CDS             2700..3362
                     /locus_tag="VIV63_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY6125014.1"
                     /translation="MISISLLSLPGAALAQAGPSDPQIIAAAPTAKINVGARKLEGHM
                     VATLRVSDDGRVRDVAVIENTAEAGFEPFLLKVMQSTRFRPAIDDSGRPIETSFDVKV
                     ELRPSTGQSPKPVAAKPDPKLTEKEKVRIRAMKCSDFVWEWKIIEDEADDRAATEFMP
                     RIAIAMYAAARTAAGEYVDAKVWKASSKALEESAKRCADSPATPFWEGVFKVVLDEAI
                     PK"
     gene            3422..4360
                     /locus_tag="VIV63_10195"
     CDS             3422..4360
                     /locus_tag="VIV63_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6125015.1"
                     /translation="MMYALSIRGLTKTYKNGVQALKGVDLDVEQGDFFALLGPNGAGK
                     TTLIGILTSLVNKSAGTVSVFGHDLDRELEAAKACIGIVPQEINFNQFETPQTIMVNQ
                     AGFYGIERTLARERTEKYLSLLQLSDKRNKIARGLSGGMKRRLMIARALVHEPRLLIL
                     DEPTAGVDIEIRRSMWEFLKDINARGTTIILTTHYLEEAESLCRNIAIINGGLIAERD
                     RMSTLLRKLQAETFVLNLREAVRAVPQIPGYEFDLIDEHTLEVEVSKEQSLNEVFAKL
                     TEQGIRVLSMRNKVNRLEELFMKLVERKPAAVVRTP"
     gene            4357..5175
                     /locus_tag="VIV63_10200"
     CDS             4357..5175
                     /locus_tag="VIV63_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6125016.1"
                     /translation="MNDTSVQTPAADSVRSLRQARWTGLKTIIIREYGRIVRIWAQTI
                     VPSAVTSALYFVIFGSLIGQRVGLMDGVPYMQYIAPGLIMMAVITNSYGNVVSSFFGA
                     KFGKHVEEMLVAPLPNWIIIIGYVGGGLVRGFLVGGAVTIVSLIFTNLAVHHAFVILA
                     AVVLTSVVFSLGGFINAQFAKNFDQVNWIPTFVLTPLTYFGGVFYSISLLPEWARHLS
                     YANPILHMVNAFRYGFLGTSDVNVYVAFGIMLASAVVLFAVALFFMNRGTGMRE"
     gene            complement(5176..5412)
                     /locus_tag="VIV63_10205"
     CDS             complement(5176..5412)
                     /locus_tag="VIV63_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125017.1"
                     /translation="MNVFNTSHFRLSIAAVASTLLLAGCGLAETAAVTATQAESAAEQ
                     AKQGKEMEAKVQRDIEAANKVAADARAKAEEESQ"
     gene            complement(5409..6287)
                     /locus_tag="VIV63_10210"
     CDS             complement(5409..6287)
                     /locus_tag="VIV63_10210"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6125018.1"
                     /translation="MTDAGPVPETGAGPVFFRRRVFLIAAALLILLACIAIYLVTALQ
                     KFLTFPGAHPSVQRLAALDEARGESMWLDVDGDRVEAWFLPAAGAPPHSLLIHAHGNG
                     ELIDIQTKSVEPLRAAGVSVLLVEYPGYGRSGGSPSEESVTATFVAAYDRMKGDPRVD
                     AGHIMGYGRSLGGGAVAQLAAHRPLAALVLESTFTSIARMVRDAGVPEWLVINDFDTS
                     AVLAKYSGPVLVLHGTRDQTFPVEHGHALRAASPHSELHIEVCGHNDCPSQWELVLSF
                     LARNGVLNEPVPGGAP"
     gene            complement(6297..6917)
                     /locus_tag="VIV63_10215"
     CDS             complement(6297..6917)
                     /locus_tag="VIV63_10215"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEY6125019.1"
                     /translation="MRRVLPLKIALASLLIAACVTINVYFPAAAADKAADQVINDITG
                     GGSKSSTTPPQSYFVPANGEHNVLVASLGNVLYAMVPAANAQDADAALNVSSPAVTRI
                     KQSMGARFGEMEKFFASGAIGLTKDGLVDVRDLNAVALPDRATVKRLVAEDNADRTQL
                     YAEIAKASNHPEWEADIKKSFAKRWVATGAKPGWYYQGEDGSWKQK"
BASE COUNT         1243 a         2275 c         2172 g         1303 t
ORIGIN      
        1 gttgtcgtcg ctgcgcaggc aatgggcctc gatgccggca cacgccgtgc gccgactctc
       61 cgccgcgcca ttcagtcttt tcgatgccgg cttcgcgtcg gcgccgaggt ggctcgaagt
      121 gcgcacgcgt ggcgtccatg aactgccgca acgtgtcgag ccggccttct tcccgccgga
      181 gatggcgcgt tccatcacgc gccgcatgct ggtgtacgga tggcacctcg cgcgcagccg
      241 tccgcgcgtg gcttgtctgg ttttcggttc gaccatggca ggaatcgaat cgctggcggt
      301 gtgttcgctg gcgtcgctgg aagccgcggc cgatcgaaac gcggaatcac tgcggccccg
      361 ctggtcgagc cagacccatt tctggcgcga gttgctgctc gctgcgtcag cgggcgctga
      421 ccggcggctg cacgaactac tgctcggcgg agtgcaacgc cttgccgcgg aaacactcgc
      481 ggcgcattct ccttagcacc tgtctgcacg cgccgctcgg gaggaaaagc gtgtgtggtg
      541 agaagccacc gaacaatgcg ggcggagccc gcgcaattca cacgactcgg ggagcacatg
      601 actaacaacc gatccgccat cgcggcaatc gccgccattc tgttgtccat ttcttccggc
      661 gccagtgccg cgtcgccgga cgacgccatc gccgcgcgga acgcggcgaa atccggcgag
      721 ggtgcgcggc tcggcgccag cctggccgtg ctgcagaaag ccgccgcgag gcgagtaccg
      781 cagcccgatg tcacccgtcg agtgacgcgc aaacttcccg ccctgcgcgc gagccagggt
      841 tacgtttcgg tcagcgcgta cggtgacgac ctgcccgcgc tgcgaaccca gctcgaatcc
      901 aggggactca tcgacgccgt cgtgcatccc accgccgtgt ccggcaaggc gccggtggcg
      961 gcgctcgccg acatggccag aaccagcggg ctcaagtttt tgcggcccac gctcgccatg
     1021 gcgcgcgctt cgcggggggc catcgtttcg cagggcgatc gttctcttcg tgcggatcgc
     1081 gcgcgacgcg atagcggcgc aaccggacga ggcattcggg tcggagtgct gtccgacagc
     1141 tacgactgcg cgccggggcc cttcgagcct ggctcaccgt tcacgcgtgc ggccgaagac
     1201 atcgccaacg gcgacctgcc gcgtgacgtc ctcgtcctca aggatctcgc ctccgcaccg
     1261 agcggcgact gctccgacga aggccgggca atgatgcaac tcgttcacga cgtggcgccc
     1321 ggatcgccgc tcgcgttcta cacggccttt gaaagtcagg aagacttcgc ggcgggaatt
     1381 cgtgcgctgg ccgcggcggg ctcgaaagtc atcgtcgacg acatcatcta tttcgccgag
     1441 cccatgttcg aagacggcat catcgcgcag gcggtcgacg acgttcatgc cgcgggtgtc
     1501 gcgtacttct cctcggccgg caatgacgcg cgcctgtcct acgaatcgcg ttatcgccaa
     1561 tccgccgagc ccggcatctc cggtccgcgc cacgacttcg cgccgggcgg ccgcgtcgat
     1621 ggcctgcaga ccgcgaccgc atcagccggc agcgccacgc tgttgtcggt ccagtgggat
     1681 caggcatcgt tgtcggccaa cggccgccgc ggagcgaaaa gcgacgtcga cgtctggttc
     1741 tacgacatga acggcgaccc catcgagatc tgcaccgacg atcccgcgca gttgatctgc
     1801 cagatccccg ggttcgacgc caacatcgac ggcgatgcgg tcgagacgcc catcatggtc
     1861 aatttcagcg acgacgacct cgaagtgcag atcggcatcg aactgttcga ggggccggta
     1921 cctaactacg tcaaatacgt atggttcgat ctcgacgcgg gcgggtttac ggtcgacgaa
     1981 tacgacacgg ccagcggtac ggtttatggc cacgccaatg ccgccggtgc ggaagcggta
     2041 ggcgccgccg cctggtacca gacggaagag tggggcagcc cgctgcgtcc gcagtgcatt
     2101 ccggcttgtc ttaattcgtt ttcgtccgcg ggcggaaccc cgctcttttt cggcccaaac
     2161 gggcgccggt tgagcgcgcc cttggtgcgc ctcaagccgg gcatcacagg accggacgga
     2221 ggtaacactt cttcattctt cttcgacctc ggcttcgaaa taccgggaac ttccgagccc
     2281 gacggcattc ccaacttctt cggtacgtcg gcttcggccc cacacgtggc cggggtggcc
     2341 gccctgatgc tcgaccagag ggcgcgagac gtggcagccc gtaagcgttt catcggtccc
     2401 cggactttga ctcccgattt gatttattgg gccatgcgcc tgacggcgga tgacatgaaa
     2461 ctgcgtaact tcgggggcga catcggcccg caacgcgtcg ataatgccaa cggatacgat
     2521 ttcgacaccg ggttcggatt cgtggacgcg cagcgggcgc tgcgcgcaac tcgcggcttc
     2581 taacaagcca tacggctttc cggagatgct ggttgcgacc ggcatccgtc gcttgcggcg
     2641 ggtctcggca cttatgcagg aatggagtcg gctaacacat gctttcaact gcgcgcgcag
     2701 tgatctcgat cagtcttctg tctctacccg gcgctgccct ggcgcaagcc ggtcccagcg
     2761 atccccagat catcgctgcc gcacccaccg ccaagatcaa tgtcggcgcg cggaaactgg
     2821 agggacacat ggtggccacc ttgcgcgtca gcgacgacgg ccgcgtgcgt gacgtcgcgg
     2881 tcatcgagaa caccgccgag gccggcttcg agccgtttct cctcaaggtc atgcaaagca
     2941 cccgttttcg accggccatc gacgacagcg gccgtcccat cgaaaccagt ttcgacgtga
     3001 aggtcgagtt gcgtccgtcg acgggtcagt cgcccaagcc ggtcgcggca aagcccgatc
     3061 cgaaactcac cgagaaagag aaggtgcgca tccgcgccat gaagtgttcg gatttcgtct
     3121 gggaatggaa aatcatcgaa gacgaagccg acgatcgcgc ggccaccgaa ttcatgccgc
     3181 gcatcgccat tgccatgtac gccgccgcgc gcaccgcggc gggcgaatac gtagacgcga
     3241 aagtctggaa agcctcgtcg aaggcgctcg aggagtccgc gaagcgttgc gccgatagtc
     3301 cggcgacgcc gttctgggaa ggtgtcttca aggttgtcct ggacgaagcc attcccaaat
     3361 aggccgcgcc tccctccaat gaaacattga tttcgcccag cgatccttca agctgccaga
     3421 catcatgtat gcactgtcga ttcgcggcct caccaagacc tacaaaaatg gtgttcaggc
     3481 cctcaagggc gtcgatctcg acgtggagca gggcgacttc tttgccttgt tagggcctaa
     3541 cggcgccggc aagacgacgc tcatcggcat cctgacttcg ctcgtgaaca aatccgcggg
     3601 tacggtcagc gtgttcggtc acgatctcga ccgcgagctc gaagccgcca aggcgtgcat
     3661 cggcatcgtg ccgcaggaaa tcaatttcaa tcagttcgag acgccgcaga ccatcatggt
     3721 caatcaggcc ggcttctacg gcatcgagcg cacgctggca cgcgaacgaa ccgaaaagta
     3781 cctgtcgctg ctccagctct ccgacaagcg caacaagatc gctcgcggtt tgtcgggcgg
     3841 catgaaacgc cgcctgatga tcgcgcgtgc actcgttcac gaaccccgcc tgctgatcct
     3901 cgacgagccg accgcgggcg tcgacatcga aatccggcgc tcgatgtggg aattcctcaa
     3961 agacatcaat gcacgcggca ccaccatcat cctgactacg cactacctcg aagaagccga
     4021 atcgttgtgc cgcaatatcg ccatcatcaa cggcgggttg attgccgagc gcgaccgcat
     4081 gtcgacgctg ctgcgcaagc tgcaggcaga gaccttcgtg ctcaacctgc gcgaagcggt
     4141 gcgggccgtg ccgcagattc cgggttatga attcgacctc atcgacgagc acaccctcga
     4201 ggtcgaggtc tcgaaggaac agagcctgaa cgaagtgttc gcaaagctca ccgagcaggg
     4261 catccgcgtg ttgtccatgc ggaacaaggt caaccggctc gaagagctct tcatgaagct
     4321 ggtcgagcgc aagcccgccg ccgtggtgcg cacaccatga acgacacttc ggttcaaact
     4381 cccgccgcgg actcggtgcg ttcgttgcgc caggcgaggt ggacgggcct caagaccatc
     4441 atcattcgcg agtacggccg catcgtgcgc atctgggcgc agaccatcgt gccgtccgcg
     4501 gtcacgtcgg cgctgtactt cgtcatcttc ggtagtttga tcggacagcg cgtcgggctc
     4561 atggatggcg tgccctacat gcagtacatc gcgcccggcc tcatcatgat ggccgtcatc
     4621 accaactcct acggcaacgt ggtgtcgtcg ttcttcggtg ccaagttcgg taaacacgtc
     4681 gaagagatgc tggtcgcgcc gctgccgaac tggatcatca tcatcgggta tgtcggcggt
     4741 ggcctggtgc gaggcttcct ggtgggtggc gccgtcacca tcgtctcgct gatcttcacc
     4801 aacctcgccg tgcaccacgc gttcgtgatt ctcgccgcgg tggtactgac gtcggtggtg
     4861 ttctcgctcg gcggattcat caacgcccag ttcgccaaga acttcgacca ggtgaactgg
     4921 attccgacgt tcgtgctgac gccgctcacg tacttcggcg gcgtgttcta ttccatctcg
     4981 ctgttgccgg agtgggcgcg gcacctttca tacgcgaacc ccatcctgca catggtgaac
     5041 gcgttccgtt acggcttcct cggcaccagc gacgtcaacg tgtacgtcgc gttcggcatc
     5101 atgctggctt cggcggtcgt gctgttcgcg gtggcgctgt tcttcatgaa ccgcggcacg
     5161 ggcatgcgcg aatagttact ggctctcttc ctcggccttt gcgcgggcgt cggccgctac
     5221 cttgttcgcc gcttcgatat cgcgctgcac cttcgcctcc atctccttgc cttgcttggc
     5281 ctgctcggcg gccgattcgg cctgagtggc agtgaccgca gccgtttccg caagtccgca
     5341 gccggcgagc agaagggtgg atgccaccgc ggcaatgctc aatcgaaagt ggctggtgtt
     5401 gaaaacattc atggcgcgcc tccgggtact ggctcgttta atacgccgtt ccttgcgagg
     5461 aaactgagta ccagctccca ctgcgaagga caatcgttgt gaccacacac ttcgatgtgc
     5521 agttctgaat gtggcgacgc cgcacgcagc gcatggccat gttccaccgg aaaggtctga
     5581 tcgcgcgtgc cgtgcagcac gagtaccggc cctgaatatt tcgcgagcac cgcgctcgta
     5641 tcgaaatcgt tgatcaccag ccactcaggc acaccggcat cccgcaccat ccgggcaatg
     5701 ctggtgaacg tggattcgag cacgagagcc gcgagcggac ggtgtgcggc gagctgcgcc
     5761 acggcgccgc cgccgagcga gcgcccataa cccatgatgt gtccggcatc cacgcgcggg
     5821 tcacctttca tgcggtcgta ggctgcgacg aacgtggccg tgacgctttc ttcggatgga
     5881 ctgccgccag agcgcccgta ccccggatac tcgacgagca gcacgctgac tcccgcggcg
     5941 cgcagcggct cgacggattt cgtctggatg tcgatgagtt cgccattgcc gtgcgcgtgg
     6001 atcagcaacg aatgcggtgg cgcgccggcc gccggcagga accacgcctc gacgcgatcg
     6061 ccgtcgacgt cgagccacat cgactcgcca cgcgcctcgt cgagggctgc cagccgctgc
     6121 accgaaggat gcgcgcccgg aaaggtgagg aatttctgca gtgccgtgac caggtagatc
     6181 gcgatgcagg cgagcaggat caacagggct gccgctatga ggaatacacg gcgccggaaa
     6241 aaaacagggc ccgctccggt ttccggaacg ggccctgcgt cggtcatcta actagattac
     6301 ttctgcttcc aggagccgtc ttcgccctgg tagtaccagc cgggcttcgc gccggtggcg
     6361 acccagcgct tcgcgaagct cttcttgatg tcggcttccc actccgggtg gttgctggcc
     6421 ttcgcaatct cggcatagag ctgcgtacga tcggcgttgt cttccgccac caggcgcttg
     6481 accgtggcgc gatcgggcag ggcgaccgcg ttgaggtcac gcacgtcgac caggccgtcc
     6541 ttggtgaggc cgatggcgcc gctcgcgaag aatttctcca tctcgccgaa acgcgcgccc
     6601 atggactgct tgatgcgcgt gaccgccggc gagctcacgt tgagagcggc gtccgcatcc
     6661 tgcgcattcg cggcaggcac catcgcgtac aacacgttgc cgagcgaggc gacgagcacg
     6721 ttgtgttcgc cgttggccgg cacgaaatag gactgcggcg gcgtggtgct gctcttggag
     6781 ccaccgccgg tgatgtcatt gatcacctgg tcggccgcct tgtcggcagc agcggcaggg
     6841 aagtagacgt tgatggtgac gcacgcggcg atcagcaaac tcgccagtgc aatcttcagc
     6901 ggcaatacgc gtcgcattga taactcctta ttccaagcgc aggcctggac gcggacatga
     6961 tcgggcttga tcgatgaatg cgccctgaac gtg
//
LOCUS       DATDEU010000427         3270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_664718, whole genome shotgun sequence.
ACCESSION   DATDEU010000427 DATDEU010000000
VERSION     DATDEU010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664718"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VIV63_10220"
     CDS             <1..864
                     /locus_tag="VIV63_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6125020.1"
                     /translation="TRCSMWIRVFSRFPLALWYGFARFMAWCAWKPFPYRRHVVEANL
                     KISFPEWDDAARELVIRDYYRGFADVFVEVFCSLRLSREELARRLVLKNPEVIRDEAA
                     RGKPVLLLGAHQCNWEWMLLALSAQLGMPLDAAYKPLTDSWAECELRKLRSRFGARMI
                     PAAELLGDIIKQRGTPRGIAVLADQEPKPGERKQWVRFLNRDSAFFVGLEEIARATRY
                     PAYFLAIRRTRRGHYEVEFVRIAAAGEQLPAGEFTTRYASMVEAQIRAAPADWPWSHK
                     RWKLKKSLYGN"
     gene            1640..3004
                     /locus_tag="VIV63_10225"
     CDS             1640..3004
                     /locus_tag="VIV63_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6125021.1"
                     /translation="MTQHTTTHETSASLLAPLARGLLRRQPEILSLSIHDRHGHALWS
                     SGDFLLAEDHALIAEVIELSVIDEPPDVRGPDCGLWWESADTARARCALPIHEGETFC
                     GVVLIAFSGLTTCDEDRVTRVEELAPVLPVLARTIRQAPDFIVDDPDLATDGRTGHTT
                     IIAALRFDRHDDDELAKDLEILAREERLLSLIDSALKEDEFALHLQPIVSLRDDASVN
                     GKPLIVEVLIRLPTQEFGVLSSHEFLETADRNGRMPAIDRWVIRALLVWMQRNRDRWA
                     DANAVFSVNLSGKSVVRPDFRNYLEHCLDKSGLPLDSIRFEVAELDAGIAPQEFAALA
                     RSLIERGCEVSVDNAGTGTGRFDFLREVNADILKIDGSLVNGASDDIVSRALINGLVH
                     MADTLGMRTVASQVDSTGKLRAVTQLGVDYAQGYRVHRPEHIDEFAFVDCGIQRPLET
                     VARA"
     gene            complement(3011..>3270)
                     /locus_tag="VIV63_10230"
     CDS             complement(3011..>3270)
                     /locus_tag="VIV63_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY6125022.1"
                     /translation="ALARAQCFPGAHASSGYALFAFYFVFRDRSRRVARWLLAAAIAV
                     GVAFSIGQEARGAHFLSHDLASAAIVWFVQLALYRRILKPD"
BASE COUNT          573 a         1106 c         1015 g          576 t
ORIGIN      
        1 accagatgtt cgatgtggat tcgcgtgttc tcgcgtttcc cccttgccct ctggtacggc
       61 ttcgcccgtt tcatggcgtg gtgtgcgtgg aagccattcc cgtatcgccg tcatgtcgtc
      121 gaggcaaacc tcaagatctc gtttcccgaa tgggatgacg ccgcgcgcga actcgtgatc
      181 cgcgactact accgcggctt cgccgacgtg ttcgtcgagg tgttctgctc tttgcgtctc
      241 tcgcgcgaag agctcgcccg gcgtctcgtg ctcaagaacc ccgaggtcat ccgcgacgaa
      301 gcggcgcgcg gcaaacccgt gttgctgctc ggcgcgcacc agtgcaactg ggaatggatg
      361 ctgctcgcgt tgtcggccca gctcggcatg cccctcgacg ccgcctacaa gccactgacc
      421 gacagctggg cggaatgcga gttgcgcaag cttcgctcgc gtttcggcgc gcgcatgatt
      481 ccggccgccg agttgttagg cgacatcatc aagcagcgcg gcacgccgcg cggaatcgcc
      541 gtgctggcgg accaggaacc caaaccgggc gaacgcaagc agtgggtccg tttcctgaat
      601 cgcgacagcg cgttcttcgt ggggctcgag gagatcgcgc gcgccacgcg ttatcccgcg
      661 tacttcctcg cgatcaggcg cacgcgccgt ggtcactacg aagtggagtt cgtcaggatc
      721 gccgcggccg gtgagcagtt gcccgccggt gaattcacca cgcgttacgc cagcatggtc
      781 gaagcgcaga tccgtgccgc acccgccgac tggccgtggt cgcacaagcg ctggaagctc
      841 aagaaatcgc tgtacgggaa ttaatgggga cattcctgat ttgctagcaa aaagggacgc
      901 gcctcaagca gtaagggcga gcgactgcga cccctgcggg cgatgagcct cgcttttcgc
      961 cgggcccttc gttcaggaag ttggcagcgc tagtttgctg gccgccgcct gacacgaaga
     1021 catttggtcg tagcagcgcc aatcgttagc aaagagtcca tttgaagcag agaggcggct
     1081 tcgtgcctag cgaggtcagg ggcccggccc gctcacctca tcacccgcag gggtcccagt
     1141 cgctctcgcg gtaccccatg atccccggct ccgttcacca aagaatcagg cacgtagccc
     1201 cggctgtgaa aggacctggc gtctagcgtc tcttctcttc tcaccgcagc ggggaatcgg
     1261 agcggggttc gagggtgtac gcgccccagc gagcgcacac gcgcaggagg tcgagagcgg
     1321 gccgggctcg tgacctcgct aggcacggag ccgcctctcc gcttcatcag gactctttgc
     1381 taacgccccg cccatctacg ccccgatgtc ttcgtgtcag gcggcggcca gcaaactagc
     1441 gctgccagct tcccgaacga agggcccggc gaaaagcgaa cgacctcctg cgcgggggcg
     1501 atcgctgggg ccctcctatc ttccaactcc tccatccatt tgacctaacg ccgccgaacc
     1561 gcctccagtc gcccgaaccc cacgtcgctc cctcgcgacg acgtcatcgc ctgcggggcc
     1621 ccctaacctt gggccacgta tgacgcaaca cacgacgaca catgaaactt ccgcttcgct
     1681 gctcgcgccg ctggcgcgtg gcctcctgcg ccgccagccc gaaatacttt ccctgtcgat
     1741 ccacgatcgt cacggccatg cgctctggtc gagcggcgac ttcctgcttg cggaagatca
     1801 cgcgctgatc gccgaagtca tcgaactatc cgtcatcgac gaaccgcccg acgtgcgcgg
     1861 gccagactgc ggcctgtggt gggagagcgc ggataccgcc cgcgcacgtt gtgcgcttcc
     1921 cattcacgaa ggtgagacgt tctgcggcgt cgtcctgatc gcgttctcgg gcctcacgac
     1981 ctgcgacgaa gatcgcgtga cgcgcgtgga ggagctcgca cccgtactgc cggttctcgc
     2041 ccggacgatc cgccaggcgc cggacttcat cgtggacgat ccggatctcg ccaccgacgg
     2101 ccgcaccggc cacaccacga tcatcgcggc gctgcgcttc gatcgccacg acgacgacga
     2161 gctggccaag gatctggaaa tcctcgcccg cgaagagcgc ttgctgtcgc tcatcgactc
     2221 ggcgctcaag gaagacgaat tcgcgctgca cctgcagccc atcgtttcgt tgcgcgatga
     2281 cgcctcggtc aatggcaagc cgctcatcgt cgaggtgctg atccgcctgc cgacgcagga
     2341 gttcggcgtg ctgagctcgc acgaattcct cgaaaccgcc gatcgcaacg gccgcatgcc
     2401 cgccatcgat cgctgggtga tccgcgcgtt gctcgtgtgg atgcagcgca atcgcgaccg
     2461 ctgggcggat gcgaacgcgg tgttctcggt caacctgtcg ggcaagtccg tcgtgcgtcc
     2521 cgacttccgc aactatctcg agcactgcct cgacaagtcg gggctgccgc tcgattcgat
     2581 tcgtttcgaa gtggccgagc tcgacgccgg catcgcgccc caggagttcg ccgcgctggc
     2641 ccgttcgctc atcgaacgtg ggtgcgaagt ctcggtggac aatgccggca ccgggaccgg
     2701 ccgcttcgat ttcctgcgcg aagtgaacgc cgacatcctg aagatcgacg gctcgctggt
     2761 gaacggcgct tccgacgaca tcgtctcgcg cgcgctcatc aacgggctcg tgcacatggc
     2821 cgacacgctc ggcatgcgca cggtggcgag ccaggtggat tccaccggca aactgcgcgc
     2881 ggtcacgcag ttgggcgtcg actatgcaca gggttatcgc gtgcacaggc cggaacacat
     2941 cgacgaattc gccttcgtcg attgcgggat tcagcgcccg ctggaaacgg tcgcgcgcgc
     3001 ctgaggcagg tcagtcgggc ttgagtattc tccggtagag cgcgagctgc acgaaccaga
     3061 cgatcgccgc gcttgcgaga tcgtgcgaca ggaaatgcgc gccgcgtgcc tcctggccaa
     3121 tcgagaacgc gacacccacc gcgatggctg ctgccagcaa ccagcgcgcc acgcggcgtg
     3181 aacgatcgcg gaatacgaaa tagaacgcga aaagcgcata acccgaagac gcgtgcgcac
     3241 cggggaaaca ctgggcgcga gccagcgcgt
//
LOCUS       DATDEU010000428         9849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_810957, whole genome shotgun sequence.
ACCESSION   DATDEU010000428 DATDEU010000000
VERSION     DATDEU010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810957"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /gene="metK"
                     /locus_tag="VIV63_10235"
     CDS             complement(<1..861)
                     /gene="metK"
                     /locus_tag="VIV63_10235"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY6125023.1"
                     /translation="MSNTFHFTSESVSEGHPDKVSDQISDAVLDALLAQDKFSRVGAE
                     TLCNTGLVVLAGEITSSAVVDFQQVARDTIKKIGYDNTDYGIDYKGCAVLVAYDKQSP
                     DIAQGVDEGKGLNLEQGAGDQGMMFGYACTETEGLMPLPIYLSHRLVEKQAELRKNGK
                     LKWLRPDAKSQVTVKYVDGKPSEIDTIVLSTQHHPDVEHKHIEQAVIEEIIKPVLAGQ
                     TLAKNIKYLVNPTGRCVIGGPQGDCGLTGRKIIVDTYGGAAPPGGGAFSGKDPSKVDR
                     SAAYAARYVAK"
     gene            complement(872..1837)
                     /locus_tag="VIV63_10240"
     CDS             complement(872..1837)
                     /locus_tag="VIV63_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY6125024.1"
                     /translation="MPGKDKSALICGSVAYDTIMVFPDRFKAHILPDKLHMLNVAFLV
                     PQMRREFGGCAGNIAYNLKLLGDTGYPMGGIGIDFAPYSAWMKKQGMSEKYLVSIPDE
                     HCAQAFITTDLDSNQITAFHPGAMNHSARIKVPANDGISIGVIAPDSREGMLQHAEQF
                     KAAGIPFIFDPGQAMPLFAGDDFKRFISISDWLALNDYEWALLQERTGWSVADVMKHV
                     KALIVTRGGEGSTIHADGKTYEIPCAKALEVLDPTGCGDAYRAGLIHGILRGLDWETT
                     GRIASLMGAIKIAVRGTQNHTFTQAEFADQYRAAFGASLNSQDIR"
     gene            complement(2087..3952)
                     /locus_tag="VIV63_10245"
     CDS             complement(2087..3952)
                     /locus_tag="VIV63_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY6125025.1"
                     /translation="MAFGAAAAIAANQSPLSPDFDARNWASIGRTAGETHYSPLTEIN
                     RETVGRLKLAWTLDLDVGNAQSTPLAVDGVLYVAAGYSMVYAVDAITGKTLWQFDPGV
                     TKVAGKKLRAGSGIRGLAYSTGRLFVGTHDGRLIALDAKKGAQVWSTPVLDANDSTFI
                     SGAPRVFKGKVAIGFGDSGATPGRLSVFDAATGNPVWNWTAQGGGGAVWNAITYDPDN
                     NRVYAGTGNARGSDAAKNVFACSVVALNADTGLVEWQYDSAPGDHRQCDGSTDITLAA
                     ITLDGQPRNVILYAPKDGSFHVIDRTNGKAISTRKIGVGTHNHFAQSFSPKTGLAYVP
                     TTELPATSAAETPADAGKSALVAWDPVKQRPLWAVPTPGAFSGGVLSTAGDLVFQGQA
                     DGYITAYSASEGRRVWAFYSATAALGAPISYAIGKRQYISILNGPTQGVSGSLGAMSA
                     RFGWDSRMHPRRLLTFVLDGTGALPATPRPTFAAPVDGTGLVVDEALAKEGATAYAKC
                     EWCHGAGAIAGGGAPDLRASLSPLVAESFATVVRAGNEVRGMPKFEELSDRELEALRH
                     YLRAQARKVTRADGAPPPEPETPAPAAPAAPAEKAAEPDKPAGSLESAGTPPGRP"
     gene            complement(3988..5091)
                     /locus_tag="VIV63_10250"
     CDS             complement(3988..5091)
                     /locus_tag="VIV63_10250"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY6125026.1"
                     /translation="MTEAPKPQTNEAVRSDARKTVRVAMQEYMRRDSVVPVLRMLVDL
                     AFLGACIVGACYFEALWAKLLMVVPASVFIARLFVIGHDACHGSYTKYDWLNKIVGRI
                     AFLPSLTPFSLWDVGHNVAHHGFTNLKGRDQVWVPMSAEEWQAASKTRRVLERIYRSG
                     FGQGLYYTIEMWWKKLILPSKKHVGVRRAIFLWDNLLVLSVGAAWITALVLWAVYDNH
                     SVTWTLLCGFVLPFLAFNCIMGWVIYVQHTHPEVAWFDKREEWQKRLGFITTTINVVV
                     PKSMGRILHDILEHGAHHVNTGVPLYRLRAAQAALKTAVPHLARVYVLNWKRYWATVR
                     TCKLYDYANHQWLDFKGRVTAAPLFPESAPAAG"
     gene            5356..5880
                     /locus_tag="VIV63_10255"
     CDS             5356..5880
                     /locus_tag="VIV63_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY6125027.1"
                     /translation="MRALRAESALSLEALATRCNVSRSMISLVERGETSPTAVVLEKI
                     ATGLGCTLASLFDDASAQVSPVSRATERDAWKDPQSGYIRRNISPPNFPSPIRIVEVA
                     FPAGARVSYESGARDSAVAQQIWVRQGAIEVTVGKVTHRLAEDDCLAMQLDAPVTFRN
                     RTRKAAHYVVVLAS"
     gene            5877..6395
                     /locus_tag="VIV63_10260"
     CDS             5877..6395
                     /locus_tag="VIV63_10260"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6125028.1"
                     /translation="MSAITVRRLDIVTSREFGGLCDVLIDCVQGGASVSFMQPMTLAK
                     AADFWRGVAASVARGERALLIAEDDSGIIGTAQAVWAQPENQPHRADISKMLVHRRAR
                     RSGVGAAVLQAAERAAAEAGKSLLVLDTASADAERLYERSGWQRVGTIPDFALWPDGG
                     RCATVVYYKRLD"
     gene            6448..7743
                     /locus_tag="VIV63_10265"
     CDS             6448..7743
                     /locus_tag="VIV63_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY6125029.1"
                     /translation="MKRRELLSALGGVSALSFLPAFTRAAFAEAATLDDLTISDVEIL
                     KLSGTHELIPGLNRQYQVNPIHLYSERRPKPFVDPANGKPERRPLTHYYLRIRTKGGA
                     FGVYGMVDKEALPVLLGNLRPLVIGQNALAVERIWDQMYRSNRHSRASHYLMAISYMD
                     NALWDLRGRHFGAPVFRLLGGPTQLPVRVYGSCLGFSIDPPLAGKRAAQLKKDGFIHQ
                     KWFLGYGPGDGARGMDLNVQLVKSLREAVGDDVQIMFDAYQGWDLNYAIEWCRKVEQY
                     RPYFVEEAVPMSDLESFIRLSKATSIPIATGEHLYGRWEAEQFFKADALQFIQADPEW
                     CGGVSETVKIANLASVHGVKLLPHCHNVHAALHIVASQSPSVCPFGEFLINHVPEKQH
                     FIRDPLVAVNGLVQLSEKPGFGIEFDPAKVEKQEILVTI"
     gene            7768..9213
                     /locus_tag="VIV63_10270"
     CDS             7768..9213
                     /locus_tag="VIV63_10270"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6125030.1"
                     /translation="MSVRILAVVALCLGSFVSAQAADITASRQTAASRQARAGEAEFR
                     ALYKELVEINTTLSVGSCTKAAEAMKARLSASGFPDSDLHVIVPPERKQDGNLIALLP
                     GSDKSLKPLMLLAHIDVVEADRADWVRDPFTLIEEEGFFYARGASDDKAMAAVFTDSM
                     VRFKRDGYKPKRGIKLVLTCGEESPNVFNGVKYLIENHRELVDAEFALNEGGGGRYDQ
                     KTGVYRFVAVLAAEKLYQDYTLTTTNQGGHSSRPTPDNAIYQLVHALDKIEAHSFPVE
                     FNDTTRDYFSKFGLIEGGEKGADMIAAARSNDAAAIARLLKDPSMNAILRTNCVATQL
                     AGGHAPNALPQRATANVNCRIFPGHHPARIRDTLIAAIGDAGVKVEYQSPPETPGAPP
                     PLSRQIIGPIEALSKEMFPGVAVIPTMSSGATDGRFFTPAGIPTYGVSGMLSDGATSN
                     AHGLNERIRVQTLMEGREFLYRLTKMYASGK"
     gene            complement(9221..>9849)
                     /locus_tag="VIV63_10275"
     CDS             complement(9221..>9849)
                     /locus_tag="VIV63_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6125031.1"
                     /translation="SLQVSVARQATGEGWNRNFAGSQPQALTGKTLLVAGLGGIGLEV
                     AKRAHALGMKVIATRGSSREGPDYVSYVGLSDELPTLIPQADVVVAALPLIPATTNLF
                     DAKMFARMKKSAYFINVGRGGSVATNDLVAALNNGVIAGAGLDVTEPEPLPKDHALWK
                     AKNIIITPHMSAQSDLGQGKRMLILGEQIRRFIAGDKLLSVVDIKKGY"
BASE COUNT         1786 a         3134 c         3145 g         1784 t
ORIGIN      
        1 cttggcgaca taccgcgcgg cgtaagcggc ggagcggtcg accttggaag gatccttgcc
       61 cgagaacgcg ccgccgccag gcggcgcggc cccgccgtac gtatcgacaa tgatcttgcg
      121 gcccgtgaga ccgcaatcac cctgcggacc gccgatgacg cagcgtccgg tcgggttcac
      181 gaggtacttg atgttcttgg cgagcgtctg cccggcgagc accggcttga tgatctcttc
      241 gatcacggcc tgttcgatgt gcttgtgctc gacatcgggg tgatgctggg tcgacagcac
      301 gatggtgtcg atctccgacg gcttgccgtc gacgtacttc accgtgacct gcgacttcgc
      361 gtccgggcgc agccacttga gcttgccgtt cttgcgcagc tcggcctgct tctcgaccag
      421 gcggtgcgag aggtagatgg gcagcggcat gagaccttcg gtctcggtgc acgcgtagcc
      481 gaacatcatg ccctggtcgc cggcgccctg ctccaggttg agtcccttgc cttcatcgac
      541 gccctgcgcg atgtcgggtg actgcttgtc gtaggcgacg agcacggcgc aacccttgta
      601 gtcgatgccg taatcggtgt tgtcgtagcc gatcttcttg atggtgtcgc gagcgacctg
      661 ctggaagtcg acgaccgcgc tggaggtgat ttcacccgcg agcacgacga gaccggtgtt
      721 gcacagcgtc tcggcgccta cgcgcgagaa cttgtcctgc gcgagaagcg catcgagcac
      781 tgcgtcggaa atctggtccg aaaccttgtc cgggtgtccc tcggaaaccg actctgacgt
      841 gaaatgaaac gtgttcgaca tacgatcctc tttacctgat gtcctggctg ttgagtgacg
      901 cgccgaaggc ggcgcgatac tggtccgcga attccgcctg cgtgaatgtg tggttctgtg
      961 ttccgcgaac cgcgatcttg atggcgccca tgagcgaagc gatacgcccc gtggtttccc
     1021 agtcgagtcc gcgaagaatg ccgtgaatca ggccggcgcg ataggcgtcg ccacagcccg
     1081 tcggatcgag gacttcgagc gccttggcgc agggaatctc gtaggtcttg ccgtcggcgt
     1141 ggatcgtgga tccttcaccg ccgcgcgtca cgatgagcgc cttcacgtgc ttcatgacat
     1201 ccgccaccga ccaaccggtg cgttcctgca acaaggccca ttcgtaatcg ttcaacgcca
     1261 gccagtccga gatggaaatg aatcgcttga agtcatcgcc ggcgaacagc ggcatggcct
     1321 ggcccgggtc gaagatgaac gggataccgg cggccttgaa ctgttcagcg tgctgcagca
     1381 tgccttcgcg gctgtccgga gcgataacac caatcgatat accgtcattc gccggcacct
     1441 tgatgcgggc cgaatggttc atggcgcccg gatggaacgc ggtgatctgg ttggaatcga
     1501 gatcggtggt gatgaaggcc tgggcgcaat gttcgtcggg gatcgacacc aggtacttct
     1561 cggacatccc ctgcttcttc atccaggcgg agtacggcgc gaagtcgatg ccgatgccgc
     1621 ccatcggata accggtgtcg ccgagcagct tcaggttgta cgcgatgttg cccgcgcagc
     1681 cgccaaactc gcggcgcatc tggggcacca gaaacgcgac gttcagcatg tgcagcttgt
     1741 cgggaaggat gtgggctttg aaacggtccg gaaacaccat gatggtgtcg tacgcgaccg
     1801 atccacagat gagtgcgctc ttgtctttgc ccggcatgcc aatgggttac gcgttagaaa
     1861 cggggcgcaa agatacgcga atcggggggc cgttgcagga gagcattcgg gagcaagtgg
     1921 aaatcccttg ctgtgcgtca catgacttca taagtctttg atggaaaagg ccgttttatc
     1981 gaattcggcg acggaaccgc ctcctgggcg tcgccggtcg actcatatgt atggggcgga
     2041 gctgccccag gcgttatgga aagtggcccg cggcctcaaa ttccggtcac ggacgccctg
     2101 gcggcgtacc ggcggattcc agtgaaccgg ccggcttgtc gggttcggcg gccttttccg
     2161 cgggtgccgc cggcgcggcc ggcgcgggag tttccggttc gggtggcggc gcgccatccg
     2221 ccctcgtcac cttcctggcc tgtgcgcgca ggtaatgtcg caacgcctcg agttcacggt
     2281 cgctgagctc ctcgaacttc ggcatgccgc ggacctcatt gcccgcacgc accacggtcg
     2341 caaacgattc ggccaccagc ggcgagagtg acgcgcgcag atcgggcgct ccaccaccgg
     2401 cgatcgcgcc ggcgccatgg caccactcgc acttcgcata ggccgtcgca ccttccttcg
     2461 ccagcgcttc atccacgacg agaccggtgc catcgaccgg cgcggcgaac gtgggacgcg
     2521 gagtcgccgg caatgcgccc gtgccgtcga gcacgaacgt gagcaacctt cgcgggtgca
     2581 tgcgcgagtc ccagccgaag cgcgcggaca tcgcgccgag actgcccgac acgccctgcg
     2641 tcgggccgtt gagaatcgag atgtactggc gcttgccgat cgcgtaactg atcggcgcgc
     2701 cgagcgcggc ggtcgcggag tagaaagccc acacgcgacg gccttcactc gcggaatacg
     2761 cagtgatgta gccgtcggcc tgaccctgga aaacgaggtc gccggccgtc gacagtacgc
     2821 cgccactgaa cgcgccgggc gttggcaccg cccacagcgg tcgttgcttc acgggatccc
     2881 acgcgacgag cgcgctcttg cccgcatcgg cgggagtttc ggcagcactc gtggccggca
     2941 gctccgtggt cggcacatat gcgaggccgg ttttcggact gaacgattgc gcgaaatgat
     3001 tgtgcgtacc gacgcctatc ttccgggtcg agatcgcctt gccgttggtg cggtcgatga
     3061 cgtggaacga accatccttc ggtgcataga gaatgacatt gcgcggctgt ccatcgagcg
     3121 taatcgcggc gagcgtaatg tcggtggagc cgtcgcactg tctgtgatca ccgggcgcgg
     3181 agtcgtactg ccattcgacg aggccggtat cggcgttgag cgccaccacg ctgcaggcaa
     3241 acacgttctt cgctgcgtca gagccgcgcg catttccggt gcccgcatac acgcgattgt
     3301 tgtcggggtc gtaggtgatg gcgttccaca ccgccccgcc accgccctgc gccgtccagt
     3361 tccagaccgg attgcctgtg gcagcgtcga acacgctcaa gcgtccgggc gttgcacccg
     3421 aatccccaaa cccgatcgcg accttgcctt tgaacactcg cggcgcgccg gagatgaacg
     3481 tggagtcgtt ggcatcgagc accggtgtgc tccacacctg cgcgcccttc ttcgcatcga
     3541 gcgcgatcag gcggccatcg tgcgtgccga cgaacaggcg gcccgtggaa tacgcgaggc
     3601 cgcgaatgcc ggaaccggcg cgcagcttct ttcccgcaac cttggtgacg ccgggatcga
     3661 actgccacag cgttttgccc gtgatcgcgt ccaccgcgta caccatgctg tagcccgcgg
     3721 cgacgtagag cactccgtcc acggccaacg gcgtggattg cgcgttgccg acatcgaggt
     3781 cgagcgtcca ggcgagcttg aggcggccga cggtttcgcg attgatttcc gtgagcgggc
     3841 tgtaatgggt ttcgccggcc gtgcgaccga tcgacgccca gttgcgcgcg tcgaagtcag
     3901 gactcagcgg agactgattt gccgcgatcg ctgccgcagc accaaaagcc aaggcaacgg
     3961 cgacagctag acgcaagcga cgatggatca gccggcggcg ggcgccgatt cggggaacag
     4021 cggcgccgcg gtaactcgac ccttgaagtc gagccactgg tgattggcgt agtcgtagag
     4081 cttgcaggtg cgcaccgtgg cccagtaacg cttccagttg agcacgtaga cccgcgcaag
     4141 gtgaggaacc gcggtcttga gcgcggcttg cgcagcgcgc agccgataca gcggcacgcc
     4201 ggtattcacg tggtgagcgc cgtgctcgag gatgtcgtgc aggatgcggc ccatgctctt
     4261 cggcaccacg acgttgatgg tggtggtgat gaagcccaag cgcttctgcc attcctcgcg
     4321 cttgtcgaac caggccactt cgggatgggt gtgctgcacg tagatgaccc aacccatgat
     4381 gcagttgaag gccaggaacg gcagaacgaa gccgcagagc agcgtccagg taacggaatg
     4441 gttgtcgtag acggcccaga gcaccaacgc cgtgatccac gccgcgccga cgctcagcac
     4501 cagcaggttg tcccagagga aaatggcgcg gcgaacgccc acatgtttct tcgagggcag
     4561 gatgagcttc ttccaccaca tttcgatcgt gtagtagaga ccctggccga aacccgaacg
     4621 gtagatgcgc tcgagcacgc ggcgggtttt ggaggcggcc tgccattctt cagccgacat
     4681 cggcacccag acctggtcgc ggcccttgag gttggtgaag ccgtgatgcg ccacgttgtg
     4741 gccgacatcc cagagcgaaa acggcgtgag cgacggcagg aaggcgatgc ggccgacaat
     4801 cttgttgagc cagtcgtatt tcgtgtagct gccatgacag gcgtcgtggc cgatgacgaa
     4861 caggcgcgcg atgaacaccg aggccggcac caccatcaga agcttcgccc acaaggcctc
     4921 gaagtaacac gcgccgacga tgcacgcccc gagaaaggcg agatcgacga gcatgcgcag
     4981 cacgggaacc acggagtcgc ggcgcatgta ttcctgcatg gcgactcgca cggtcttgcg
     5041 ggcgtcggag cggacggctt cgtttgtctg gggcttggga gcttcagtca tagggccgta
     5101 agactatacg attcaagcgc tttcaccaat gcgttcggtg tcattttcga cctgcgtcgc
     5161 agatgaaaaa tacgctcggg gctccgtccg accacccggg tcagcggact tgctgcgccg
     5221 ttgtagtccc gacacgaatc gcatttccac cacacgccgt ttgcgggttt ctcgcagttc
     5281 agatttgaca cgaaatccac tttactggat gtttcgtcca ctatgcagga cataaacgcc
     5341 cgaatcgccg gccgcgtgcg cgcactgcgc gccgaatcgg ccttgagtct cgaagctctc
     5401 gccacgcgct gcaatgtcag ccgctcgatg atctcgctgg tggagcgcgg cgagaccagc
     5461 ccgacggcgg tagtccttga aaagattgct accggtctcg gatgcaccct cgcctcgctg
     5521 ttcgacgacg cgtctgcgca ggtcagcccg gtgtcgcgcg ccaccgagcg cgacgcctgg
     5581 aaggacccgc agtccggcta catacgccgg aacatctcgc cgcccaactt tccctcgccg
     5641 atccgcatcg tcgaggtggc atttcccgcc ggcgcgcgcg tgtcctacga atccggcgca
     5701 cgcgactcgg ccgtggcgca acagatctgg gtgcggcaag gcgccatcga agtcaccgtc
     5761 ggcaaggtga cgcaccggct cgcggaggac gactgcctgg cgatgcagct cgacgcgccc
     5821 gtgacgtttc gcaatcgcac gcgcaaggcg gctcactacg tcgtggtgct ggcgtcatga
     5881 gtgcgatcac cgtgcgccgg ctcgacatcg tgacctcgcg cgaattcggc gggctgtgcg
     5941 acgtgctcat cgactgcgtc caaggcggcg cgtcggtgag cttcatgcaa ccgatgacgc
     6001 tcgcgaaggc cgccgacttc tggcgtgggg tcgcggccag cgtggcgcgt ggagaacgcg
     6061 cgttgctcat tgccgaggac gacagcggca tcatcggcac cgcgcaagca gtgtgggcgc
     6121 agcccgagaa tcagccccat cgagccgaca tctccaagat gctggtgcac cgccgagcgc
     6181 ggcgctcagg cgttggcgcc gccgtactcc aggccgcgga gcgcgccgcg gcggaagccg
     6241 gcaagtcatt gctcgtgctc gacactgcga gcgccgatgc cgaaaggttg tacgagcgta
     6301 gcggctggca gcgcgtcggc acgattcccg acttcgcgtt gtggcccgac ggcggacgct
     6361 gcgccacggt cgtctactac aaaaggctgg attgacgtcg ctcgcgcggc tgcaacaagc
     6421 tcgcgcacct ttccgccaga gcgcgccatg aaacgccgag aactcttgag tgccctgggt
     6481 ggtgtttccg ccctttcctt cctgcccgcc tttacgcgcg ccgcgttcgc ggaggccgcg
     6541 acgctcgacg atctgaccat cagcgacgtc gagatactga agctgtccgg aacccacgag
     6601 ctcataccgg gactgaatcg ccagtaccag gtgaatccga tccacctgta ttccgagcgg
     6661 cgccccaagc ctttcgtcga tcccgccaac ggcaagcccg aacgccgtcc gctcacgcac
     6721 tactacctgc gaatccgcac caagggcgga gccttcggcg tctacggcat ggtggacaag
     6781 gaagcgctgc ccgtcttgtt aggtaacctg cgaccgctgg tcatcggcca gaacgcgctg
     6841 gccgtcgagc gcatctggga ccagatgtat cgttcgaatc gccattcgcg cgccagccac
     6901 tacttgatgg ccatcagcta catggacaac gcgttgtggg acctgcgcgg ccggcacttc
     6961 ggcgcgccgg tgttccgcct gctgggtggc ccgacgcaat tgcccgtgcg cgtgtacggc
     7021 agctgcctcg gcttctccat cgatccgccg ctggccggca aacgcgcggc gcagttgaag
     7081 aaggacggtt tcattcacca gaagtggttc ctggggtacg gccccggtga cggcgcacgc
     7141 ggcatggacc tgaacgtgca gctcgtgaag tcactgcgcg aagccgtggg cgatgacgtg
     7201 cagatcatgt tcgacgcgta ccagggatgg gatctgaact acgccatcga atggtgccgc
     7261 aaggtcgaac aataccgccc ctacttcgtc gaagaagccg tcccgatgtc ggacctcgag
     7321 agtttcatcc gtctgtcgaa ggcgacttcg attccgatcg cgaccggcga acacctctac
     7381 ggccgctggg aagcggaaca attcttcaag gcggacgcac tgcagttcat ccaggccgat
     7441 cccgagtggt gcggtggcgt cagcgagacc gtgaagatcg cgaatctcgc ctcggtgcac
     7501 ggcgtcaaac tactgccgca ttgtcacaac gtgcacgcgg ctttgcacat cgtcgcgagc
     7561 cagtcgccgt cggtgtgccc tttcggcgaa tttctcatca accacgtacc ggagaagcag
     7621 cacttcatca gggacccgct ggtggcggtg aacggcctgg tgcaattgtc ggaaaaaccg
     7681 ggcttcggaa tcgagttcga tccggcgaaa gtggaaaagc aggaaatact cgttacgatc
     7741 tgagctcatc cgctgtcggg agctcacatg tccgttcgaa ttcttgccgt tgtcgcgttg
     7801 tgcctgggtt ctttcgtcag cgcgcaggcc gccgacatta ccgccagccg ccagactgcc
     7861 gcttcgaggc aggcgcgcgc cggcgaagca gaattccgcg cgttgtacaa agagctcgtg
     7921 gagatcaaca ccacgctgtc ggtgggttca tgcacgaagg cggccgaggc catgaaggcg
     7981 cgcctttcgg cgtccgggtt tcccgattcc gatcttcacg tcatcgttcc gcccgagcgc
     8041 aaacaggatg gcaacctcat cgccctgctt ccgggttcgg acaaatcgct gaagccgctg
     8101 atgctgctcg cgcatataga cgtggtggag gcggaccgcg ccgactgggt acgggatccg
     8161 ttcacgctga tcgaggagga aggcttcttc tacgcgcgcg gcgcatccga cgacaaggcc
     8221 atggccgcgg tgttcaccga ctccatggtt cgtttcaagc gcgatggcta caagccaaag
     8281 cgcggcatca agctcgtgct cacctgcggc gaggaatcgc cgaacgtgtt caacggcgtg
     8341 aaatatctca tcgagaacca ccgcgaactc gtcgacgcgg aatttgctct caacgaaggc
     8401 ggtggcggac ggtacgacca gaagaccggc gtctatcgct tcgtcgcggt gctggccgcc
     8461 gagaagctct accaggacta cacgctgacg acgaccaacc agggtggcca ctcttcgcga
     8521 cccacgcctg acaacgcgat ctaccagctc gtgcacgcgc tcgacaagat cgaggcgcat
     8581 tcctttccgg tggaattcaa cgacaccacg cgtgactatt tctcgaagtt cgggctcatc
     8641 gaaggcggcg agaaaggcgc ggacatgatc gcggcggcca ggagcaacga tgcggcggca
     8701 atcgcccgcc tgctcaagga tccgtcgatg aacgccatcc tgcgcaccaa ctgtgtagca
     8761 acgcagctcg cgggcggcca cgcaccgaat gcgctgccgc agcgcgcgac ggccaacgtg
     8821 aattgccgaa tctttccggg ccaccatccg gccaggattc gcgacacgct gattgccgcc
     8881 atcggcgacg cgggcgtgaa agtggaatac cagtcgccac cggaaacacc gggcgcgccg
     8941 ccgccgctgt cgaggcagat catcgggccc atcgaggcgc tctcgaagga aatgttcccg
     9001 ggcgtcgcgg tcattccgac catgtcgtct ggcgcgaccg acgggcgctt cttcacgccg
     9061 gccggcattc ccacctatgg cgtctccggc atgttgagcg acggcgcaac cagcaacgca
     9121 cacgggctca acgagcgcat ccgcgtgcag acgctcatgg aaggtcgcga gttcctttac
     9181 cgcctcacaa agatgtacgc gagcgggaag taacgcccgc ctaataacct ttcttgatgt
     9241 cgacgaccga cagcagcttg tcgccggcga tgaaccgccg gatctgttcg cccaggatga
     9301 gcatgcgctt gccctgcccc aggtccgact gcgccgacat gtgcggcgtg atgatgatgt
     9361 tcttcgcctt ccacaaggcg tgatccttcg gcagcggttc gggctcggtc acatcgagcc
     9421 cggcgccggc gatgacgccg ttgttgagcg cggcgacgag gtcgttggtg gcgacgctgc
     9481 ctccgcggcc cacgttgatg aagtacgcgg acttcttcat gcgcgcgaac attttcgcgt
     9541 cgaagagatt cgtagtcgca ggaatcagcg gcaaagcggc caccacgacg tcggcttgcg
     9601 gaatcaacgt cgggagttca tcggagagcc cgacgtaact cacgtagtcc ggtccctcgc
     9661 gcgaactgcc gcgggtcgcg atgactttca tgccgagcgc atgtgcccgc ttggcgactt
     9721 cgaggccgat gccgccgaga cccgcgacca gcagggtctt gccggtgagt gcctgtggct
     9781 gcgagcccgc gaagttgcgg ttccagccct cgcccgtcgc ctgacgggcc acggacacct
     9841 gcaggctgc
//
LOCUS       DATDEU010000429         7776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_371837, whole genome shotgun sequence.
ACCESSION   DATDEU010000429 DATDEU010000000
VERSION     DATDEU010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371837"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VIV63_10280"
     CDS             <1..207
                     /locus_tag="VIV63_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125032.1"
                     /translation="GREHDVVYSDPPPADVKRFLEGYDRFAEQSAGKLNRFGIEGVST
                     YHHARDVVNRNALHGKIDCDGGAT"
     gene            204..1412
                     /locus_tag="VIV63_10285"
     CDS             204..1412
                     /locus_tag="VIV63_10285"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY6125033.1"
                     /translation="MSGALDGIRVVDFSHVMAGPYASHLLRLLGAEVIKIEAPGMGDA
                     MRNYGPDRRYDGMAPAFVAVNAGKKSLVLNLKHPPAREAARRLIARADVVLENFRPGV
                     MARLGLDYQSVRALQPRMVYCSVSGYGQHSPRRDWPAIDNIVQATSGMMSLSGEQGDD
                     PMRVGFPVVDTLTGQTAAFAILAALLRRERTGAGELIDVAMFDAAIAFMTSAVVPYLV
                     TGRGLARTGNVGYSGQPTSGVFATSDGRQVSLGVVQQPQFEALARLVGRQDWLADPRY
                     ANADLRRQHATEIHGELSALFATRPAAQWEQSLSEAGIPCGMVREVSEAIGLSGLEER
                     GIKLPLHVPSLPDCKDVSIVNAGFKFSEDSPHLDVAPPRFGEHNEEILTSLGFDDAER
                     REILAGNAEK"
     gene            1423..3159
                     /locus_tag="VIV63_10290"
     CDS             1423..3159
                     /locus_tag="VIV63_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY6125034.1"
                     /translation="MQIHAALALVTLALFTAVASAAEPRVSRPGEYRGYSEAVFDGHQ
                     LSSRYVQVRDGTRLAIDIFEPTKGGKVAAGRYPVVWMHTPYNRRDTQGGLTAANYPGK
                     ALQLVKYGYVVAVADFRGTHASFGRNAGFNRGEWQDAARFDAYDITEWLASQPWSNGK
                     VGMWGCSATGGSQMQALSTAPPHLKAIFPMSCEWDVYSWVAAGGMTPPPGQPTQIMRG
                     APREERDRNAVAVDDDRDGALLRAATGEHRDNLETPGFVPYRDSVSEAFGNAWWVKSS
                     PSTYRGDINKSGIAVYAATNWAEGFTGYGPPFTFNNLRTPKKLIFGPGKHCDWSTVLK
                     DTGFDIVVEELRFFDHWLRGIDNGVMREPAVTYYTYNEAPAKAWKSSRTWPLKNERRT
                     RFYLGDGTLDSRAPAAGESRMQVIYGVDDASFWKSGMTFVSAPLEHDMEVTGHPVAHV
                     WLASTTTDADVIARIDDVAPDGTSRYAGVEGKLRASMRALGKPPYDNLGLPWHPGTEA
                     SKLPLQPDQPVELTFDLMPMSYLFKAGHRIRVTMQFADARATAKVDPAPVVTVLHRKG
                     AASSIELPVIPR"
     gene            complement(3164..4780)
                     /locus_tag="VIV63_10295"
     CDS             complement(3164..4780)
                     /locus_tag="VIV63_10295"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY6125035.1"
                     /translation="MIRNPILPGFNPDPSILRVGDDYYIATSTFEWYPGVQIHHSRDL
                     VNWKLVTRPLRRAGQLNMLGDPDSCGVWAPCLTYSDGLFWLIFTDVKRYGRTTVGGAS
                     GASLRDFHNYLVTCPTIDGEWSDALYLNSSGFDPSLFHDDDGRKYLVNQLWDHRPGRG
                     RFAGIVLQEYSPTQRKLIGETRNIFKGTAIGFTEAPHLYKRNGYYYLITAEGGTGWGH
                     AITMARSRSLLGPYELHPDKYIVTARDRPHAPLHRAGHGDLVETQAGETYVAYLCGRP
                     LPNRGRCVLGRETAIQKMVWGKDDWLRTIDGSGLPELETPAPPLPTAPFDAAPVREEF
                     NSAQLPIDFQWLRSPWPEELFSLTDRPGYLRLHGRETLGSAFRQSLVARRQQAHCYSA
                     ITAMEFEPAHFQQAAGLVCYYNSSKFHYLHVTHDEEHGRHVRVMSCVPDLPQSDFFTA
                     PIAVPAGRIELRAEVDYERLRFAYRVGNQDWTWLPELFDASALSDECTGPGSANFTGA
                     FVGMACQDVSGTAHPADFDFFEYREREYQR"
     gene            complement(4780..6060)
                     /locus_tag="VIV63_10300"
     CDS             complement(4780..6060)
                     /locus_tag="VIV63_10300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6125036.1"
                     /translation="MIGRLYALRWWMISLITIGTILNYLTRATLGVAAPTLTGELGIG
                     EQEYSWITGVFQVGIMLQPIAGYVMDLIGLKLGFGLFAIAWAMITMLHGLAFNWQILA
                     VLRGLMGFAEGSAQPGGMKAVAEWFPAKERGFAGGFYNIGASFGSMLAAPLVAWAILF
                     HSWRLAFVIAGGLALVWVVLWFKFYYSPKDHPKISAEERNYIESNQEAHLAAGTSKPS
                     IWSLLKQRNTWGIAIPRMLADPTWGTLSFWVPFYLMTERGFDIKQIALFAWLPFVTAD
                     IGCLAGPTIVLWLQRRGIGLINARRWAFTVGALLMTSMAFVGKVDSAYAAIALLCLGG
                     FAHQTLSITVITMSSDLFRRNEVATVAGIGGFCGNLGVLVFTLLIGGLVKTVGYDPFF
                     VALAVFDILGAIWLWTIVRERINPPPGPTVPVAA"
     gene            6149..6949
                     /locus_tag="VIV63_10305"
     CDS             6149..6949
                     /locus_tag="VIV63_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6125037.1"
                     /translation="MKTLPLRLIALLIGFVILTSTARAAATPDHHTFVLVHGATAGGW
                     EWKTTGKFLTEDGHTVYRATLTGLGERMHLNSADIDLQTHINDVVNLILFEDLHDVVL
                     TGHSYGGMVITGVIDRIPERIRHVVYLDAAVPDDGMSIWDLFGGNGPQDPERFKDGFM
                     QVPWVKAGATPPHSVKQSIKCFNQPVSYKNPAALALPVTYVAFVPKDKSAEERAKTDK
                     SWQRAVARGWTIRTFPGGHVAQQEDPRGVASLIEQSVNDRNKPAQKAR"
     gene            complement(7001..>7776)
                     /locus_tag="VIV63_10310"
     CDS             complement(7001..>7776)
                     /locus_tag="VIV63_10310"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6125038.1"
                     /translation="EGTGRANGMAFDDDGNLIAAADLHGELWKIRPDGSHRVLVDNYQ
                     GKLLNGPNDVWINPANGGIYITDPIFPRGYWDADDPRQQPWEPTRSEQSPQGKGGHVY
                     YLAPGARKLVRVTSDAMGWESDAWPNGVVGSPDGKKLYVNKWYYDNKGGTWVFDIRRD
                     GTLTNMRKFTDWGGDGMSMDELGNVYISNGEGVMAFDPNGNNILKIPTGSGATNNTFA
                     GRDGRTLFITGPVDRVTAIRMNVRGSENGCRRCPPWHKN"
BASE COUNT         1467 a         2494 c         2459 g         1356 t
ORIGIN      
        1 ggacgcgaac acgatgtggt ctacagcgac ccgccgccgg cggatgtgaa acgctttctc
       61 gaaggatacg accggtttgc cgagcaaagc gcgggcaagc tgaaccgctt cggcatcgaa
      121 ggagtgtcta cctaccacca cgcgcgcgac gtggtgaacc gcaacgcgtt gcacggcaag
      181 atcgattgcg acggcggtgc cacgtgagcg gtgcgctgga cggcatccgt gtcgtcgact
      241 tcagccacgt gatggcggga ccttacgcgt cgcacctgtt gcgactgttg ggcgccgagg
      301 tcatcaagat cgaagcgccg ggcatgggtg acgccatgcg caactacggg cctgacagac
      361 gctacgacgg tatggcgccc gcgttcgtcg cggtgaatgc cggcaagaaa tcgctcgtgc
      421 tcaacctgaa gcatccgccg gcgcgtgaag cggcgcgccg gctgatcgcg cgcgcggacg
      481 tggtgctgga aaatttccgg cccggcgtga tggcgcggct ggggctcgat taccagagcg
      541 tgcgcgcgct gcagccgcgc atggtctatt gctcggtgtc cggttatggg cagcacagtc
      601 cacggcgcga ctggcccgcc atcgacaata tcgtgcaggc cacgagcggc atgatgtcgc
      661 tgagcggcga acagggggac gacccgatgc gggtcggatt cccggtcgtg gataccctca
      721 ccggccagac cgcggctttc gcaattcttg ccgcgctgct gcgacgtgag cgcaccgggg
      781 ccggagaact catcgacgtc gccatgttcg acgcggcgat cgcgttcatg acgtcggccg
      841 tcgtgcccta tctcgtcacc ggacgcggcc tcgcgcgcac cggcaacgtc ggctacagcg
      901 gccaacccac ctccggagta ttcgcgacgt ccgatggtcg ccaggtgtcg ctcggtgtcg
      961 tgcagcagcc gcagttcgag gcgctggctc gcctggtcgg ccgccaggac tggctggcgg
     1021 atccgcggta cgcgaatgcg gaccttcgcc gccagcatgc gaccgagatt cacggcgagt
     1081 tgtccgcctt gttcgccacg cgccccgccg cccagtggga gcaatccctg agcgaggccg
     1141 gcattccctg cggcatggtg cgtgaagtca gcgaggccat cggcttgagc ggtctcgagg
     1201 agcgcggcat caaactaccc ctgcatgtgc cctcgctgcc ggactgcaag gacgtctcca
     1261 tcgtcaatgc cggcttcaag ttcagcgaag actcgcccca tctagatgtc gcgccacccc
     1321 gattcggcga gcacaacgag gaaatcctga cttccctcgg tttcgacgac gccgaacgcc
     1381 gcgaaatcct cgccggcaat gccgagaagt aaggagtcat ccatgcagat ccacgctgcg
     1441 ctcgcgctgg taacactcgc attgttcacg gccgtcgcct ctgccgcgga acccagggtt
     1501 tcccggcccg gtgaataccg cgggtattcc gaggcggtat tcgatggcca tcagctcagc
     1561 tcccgttacg tgcaggtgcg tgacggcacg cgcctggcca tcgatatctt cgagcccacg
     1621 aagggtggca aggtggccgc gggccgatac ccggtggtgt ggatgcacac tccgtacaac
     1681 cggcgcgata cgcagggcgg cctcacggcc gctaactacc ccggcaaggc actgcagctc
     1741 gtgaaatatg gatatgtggt ggcggtggcc gacttccgcg gcactcatgc ctcgttcggc
     1801 cgcaacgccg gcttcaaccg cggtgagtgg caggacgctg cgcgattcga cgcttacgac
     1861 atcaccgagt ggctcgcctc acaaccctgg agcaacggga aggtcggcat gtggggctgc
     1921 tcggcgaccg gaggcagcca gatgcaggcc ctcagcacgg cgccgccgca cctgaaagcg
     1981 atattcccga tgagctgcga gtgggatgtg tactcctggg tcgcggccgg cggcatgacg
     2041 ccgccgcccg ggcaacccac gcaaatcatg cgcggcgcac ctcgcgagga gcgcgatcgc
     2101 aatgccgtgg ccgtcgatga cgatcgcgac ggcgcgctgc tgcgcgccgc gacgggtgag
     2161 caccgggaca atctcgagac gccgggtttc gtgccctatc gcgacagcgt ttccgaagca
     2221 ttcggcaatg cctggtgggt caagagcagc ccgtccacct accgcggcga catcaacaag
     2281 tccggcatcg ccgtgtatgc cgcgaccaac tgggcggaag gattcaccgg ttatggtcct
     2341 cctttcacgt tcaacaatct ccgcacgccc aagaagctca tcttcggacc gggcaaacac
     2401 tgcgactgga gcacggtgct gaaggacacg ggcttcgaca tcgtggtcga ggaactgcgg
     2461 ttcttcgacc actggctgcg cggcatcgac aacggcgtga tgcgcgaacc cgcggtgacc
     2521 tactacacgt acaacgaagc gccggcgaag gcgtggaaat cctcgcgcac ctggccgctg
     2581 aagaacgagc ggcgcacgcg gttctacctc ggcgacggca cgctcgactc cagggcgcca
     2641 gccgcgggcg agtcgcggat gcaggtcatc tatggcgtgg acgacgcgag cttctggaaa
     2701 tcaggcatga cgttcgtgag tgctccgctc gaacacgata tggaggtcac cggccacccg
     2761 gtagctcacg tgtggctcgc cagcaccacc accgatgcgg acgtcatcgc gcgcatagac
     2821 gatgtggcgc ccgacggaac atcccgttac gccggcgtcg agggaaagtt gcgcgcctcg
     2881 atgcgcgcgc tgggcaagcc tccgtacgac aacctcggac tcccgtggca tccgggcacc
     2941 gaggcttcga agctgccgct tcaacccgac cagccggtcg agctcacgtt cgacctcatg
     3001 ccgatgtcct atctgttcaa agccgggcat cgcattcgcg tgacgatgca gttcgccgat
     3061 gcgcgcgcca ccgcgaaggt cgatcccgcg cccgtggtga cggtgcttca ccggaaaggc
     3121 gcggcctcga gcatcgagct gccggttatt ccgcgctaac gccctagcgc tggtattccc
     3181 gctcccggta ttcgaagaaa tcgaaatccg ccggatgcgc agtgcccgac acgtcctgac
     3241 aggccatgcc gacgaatgcg cccgtgaagt tggctgaacc gggtccggtg cattcatcgg
     3301 acagtgcgct ggcgtcgaac aattccggca gccacgtcca gtcctggttg cccacgcgat
     3361 aagcgaagcg caggcgctcg tagtccactt ccgcgcgcag ctcgatacgg cccgccggaa
     3421 ccgcaatcgg tgccgtgaag aagtcggact gcggtagatc cggaacacac gacatcacgc
     3481 gcacgtgccg tccatgctcc tcatcgtgcg tcacgtgcag atagtgaaat ttcgacgagt
     3541 tgtagtagca cacgagtccg gctgcctgct ggaagtgcgc cggctcgaac tccatcgcgg
     3601 tgatggcgct gtagcaatgg gcctgctgac gtcgcgcgac cagcgactgc cggaacgcgc
     3661 tgccgagggt ttcgcggcca tgcagccgca ggtatccggg cctgtcggtg aggctgaaca
     3721 attcttcggg ccacggcgag cgcagccact ggaagtcgat gggcaactgg gcgctgttga
     3781 attcctcccg aacgggggca gcatcgaacg gcgcagtggg caatggcggc gccggcgtct
     3841 cgagttctgg caatccgctg ccatcgatcg tgcgcagcca gtcgtccttg ccccagacca
     3901 tcttctggat cgcggtctcg cggccgagca cgcatcggcc gcggttcggc aatgggcgcc
     3961 cgcatagata ggcgacgtaa gtttcgcccg cctgggtctc caccagatcg ccgtggcccg
     4021 cgcgatgcaa cggcgcatgc ggacgatcgc gcgccgtgac gatgtatttg tcgggatgga
     4081 gttcatacgg ccccagcagc gagcgtgaac gcgccatggt gatcgcgtga ccccagccgg
     4141 tgccgccctc ggcggtgatc aggtagtagt acccgttgcg cttgtacaga tgcggcgcct
     4201 cggtgaaccc gatggccgtg cccttgaaga tattgcgcgt ttcgccaatc agcttgcgct
     4261 gcgtgggcga atattcctgc agcacgatgc cggcgaagcg gccgcggccc ggacgatgat
     4321 cccagagctg gttcaccaga tatttgcgcc cgtcgtcgtc atggaacagc gagggatcga
     4381 agccactgct gttcagatac aacgcatcgg accactcgcc atcgatcgtg ggacaggtca
     4441 ccaggtagtt gtggaagtcg cgcagcgagg cacccgaggc gccgccgacc gtcgttcggc
     4501 catatcgctt cacgtccgtg aagatcagcc agaacaatcc atcgctgtac gtgaggcacg
     4561 gtgcccacac gccgcacgaa tccggatcgc cgagcatgtt gagctggccc gcgcggcgca
     4621 gcggccgggt caccaacttc cagttaacga ggtcgcgcga gtggtggatc tgcacaccgg
     4681 ggtaccactc gaacgttgag gtcgcgatgt agtagtcgtc gcccacgcga aggatcgagg
     4741 gatccggatt gaagcccggc aggatgggat tgcggatcat catgcggcca ccgggacagt
     4801 cggacctggc ggcgggttga tgcgctcgcg cacgattgtc cagagccaga tcgcaccgag
     4861 gatgtcgaac acggcaagcg cgacgaagaa cggatcgtag ccaacggttt tcaccagtcc
     4921 gccgatcaac agggtgaaca ccagcacacc caggttgccg cagaaaccgc cgatgccggc
     4981 caccgtcgcc acttcattgc ggcggaacag gtccgatgac atcgtgatga ccgtgatcga
     5041 cagcgtctgg tgtgcaaaac ctccgaggca cagcagcgcg atcgcggcat acgcgctatc
     5101 taccttgccg acgaacgcca tgctggtcat cagcaacgcg ccgaccgtga aggcccagcg
     5161 tcgcgcgttg atgagaccta tgccacgccg ctgcagccac aacacaatgg tgggcccggc
     5221 caggcaaccg atgtccgcgg tcacgaacgg cagccaggca aacaaggcga tctgcttgat
     5281 gtcgaagccg cgctccgtca tcaggtaaaa cggcacccag aaagacaacg taccccaggt
     5341 ggggtcggcg agcattcgcg gtatggcgat accccaggtg ttgcgttgtt tgagcaggct
     5401 ccagatggac ggcttggatg tgccggccgc caggtgcgct tcctggttgg attcgatgta
     5461 gttgcgttcc tcggccgaga tcttcggatg atccttcggc gagtagtaaa acttgaacca
     5521 cagcactacc cacaccaatg cgaggccacc ggcaatgacg aaggcgagtc gccagctatg
     5581 gaacaggatg gcccaggcga ccagcggcgc cgccagcatc gaaccgaatg acgcaccgat
     5641 gttgtagaac ccgccggcga atccgcgttc tttggcggga aaccactcgg cgaccgcctt
     5701 catgccaccg ggctgtgccg aaccttcggc aaaacccatg aggccgcgca gcaccgcgag
     5761 aatctgccag ttgaacgcga ggccgtggag catggtgatc attgcccacg cgatggcgaa
     5821 cagaccaaag ccgagcttga gaccgatgag atccatcacg tagcccgcga tgggctgcag
     5881 catgatgccg acctggaaga cgccggtgat ccaggaatat tcctgttcgc cgattccgag
     5941 ttcacccgtg agtgtgggag ccgccacgcc gagcgtcgct cgagtgaggt agttgaggat
     6001 cgtgccgatg gtgatcaggc tgatcatcca ccaccgcagc gcgtagagac ggccgatcat
     6061 ggcgcaccac cgctatcgcg aaacattttc gagatcatct tccttcccct tctacaatag
     6121 tacgtcccac atacaatcgg gtcgcttcat gaaaactctt ccacttcgtc tcattgcctt
     6181 gctcatcggc ttcgtgatcc tgacgtccac cgcccgcgcc gcggcaacgc ccgatcatca
     6241 caccttcgta ctcgtccacg gcgccaccgc gggcgggtgg gagtggaaga ctaccggcaa
     6301 gttcctcacc gaggatggcc ataccgttta ccgcgccacg ctcaccggtc ttggcgagcg
     6361 catgcatctc aacagcgcgg acatcgacct gcagacacat attaatgacg tggtgaacct
     6421 gatcctgttc gaagatttgc acgacgtggt actcactggc cacagctacg gcggcatggt
     6481 gatcaccggt gtcatcgacc ggatcccaga gcgcatccgt catgtggtct atctcgacgc
     6541 tgccgtgccg gatgatggca tgtcgatctg ggatctgttt ggcggcaatg gcccgcagga
     6601 tccggagcgt ttcaaggacg gcttcatgca ggtgccctgg gtgaaggccg gcgcgacgcc
     6661 tccccacagc gtcaagcagt cgatcaagtg cttcaatcaa ccggtgtcat acaagaatcc
     6721 cgcggcgctc gcgttgccgg tgacctacgt ggcattcgtt cccaaggaca agtcggccga
     6781 ggaacgcgcg aagaccgaca agagctggca gcgcgcggtg gcgcggggat ggacgatccg
     6841 cacgtttccg ggcggccacg tggctcagca ggaagatccg cgcggtgtcg cgtcgctcat
     6901 cgagcagtcg gtgaacgacc gcaacaagcc ggcgcagaaa gccaggtagt tgccgaagga
     6961 agcccgcggt acacgccgtg ccgcgggctg atccagatga tcagttcttg tgccagggcg
     7021 gacaacggcg gcagccattc tccgaaccac gtacgttcat gcggatggcg gtgacgcggt
     7081 cgaccggacc ggtgatgaac aacgtcctgc catcccgacc cgcgaacgtg ttgttagtcg
     7141 ctcccgagcc ggtgggaatc ttcaggatgt tgttgccgtt cggatcgaac gccatcacgc
     7201 cttcgccatt gctgatgtag acgttgccca gctcatccat cgacatgcca tcgccacccc
     7261 aatcggtgaa cttccgcatg ttggtgagcg tgccgtcacg ccggatatca aagacccacg
     7321 tgccgccctt gttatcgtag taccacttgt tgacgtagag cttctttccg tcgggcgagc
     7381 cgaccacacc gttcggccat gcatcggact cccatcccat cgcatcggac gtcacgcgta
     7441 ccagcttgcg cgcgccggga gccaggtaat agacgtgacc gcctttgcct tgtggggatt
     7501 gctccgagcg tgtcggctcc cagggctgct ggcgcggatc atcggcatcc caatatcccc
     7561 gcgggaagat cggatcggtg atgtagatgc cgccgttcgc agggttgatc cacacgtcat
     7621 tcggcccgtt cagcagcttg ccctggtagt tgtcgaccag cactctgtgg ctgccgtccg
     7681 gacggatctt ccacagttcg ccgtgcaggt ccgcggcggc gatgaggttg ccatcgtcgt
     7741 cgaaggccat accgttggcg cgacctgtgc cttcca
//
LOCUS       DATDEU010000430         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1540527, whole genome shotgun sequence.
ACCESSION   DATDEU010000430 DATDEU010000000
VERSION     DATDEU010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540527"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VIV63_10315"
     CDS             <1..217
                     /locus_tag="VIV63_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125039.1"
                     /translation="IAGMNADTLSYWRRNVDFPNQTTADQFFDEEQLEAYRELGMAIA
                     QTAVRALKGEHEPWDEPMTALRRTFGW"
     gene            257..1471
                     /locus_tag="VIV63_10320"
     CDS             257..1471
                     /locus_tag="VIV63_10320"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEY6125040.1"
                     /translation="MKKLFIGLCLALAVPAAYAQKFEGLAKTPQMGWNSWNKFACNVN
                     EQMIRETADAMVSSGMKDAGYLYVNIDDCWHGERDARGFIQPHPEHFPSGMKALADYV
                     HSKGLKLGIYSDAGWKTCGGRPGSRGYEYQDALTYAEWGIDYLKYDWCDTAGLKAEGA
                     YLTMREALRKAGRPVLFSLCEWGDNQPWDWAKPVGHSWRTTGDIWDCFDCVKDHGTWK
                     SRGVLQILDLQEGLRVHAGPDHWNDADMMEVGNGGMTVNEDRAHFSIWAMLASPLIAG
                     NDIRNMSKETAAILTNKAVIALNQDVLGVQAFKFASRDGVEYWFKPLQNGDWGFMALN
                     RNADARKITFDWKAEKVNDDLSKRDAAFGKTRYSLRNLWTGRAAGHTGKALVADLPGH
                     DVLVLRLAAPGR"
     gene            complement(1479..>2363)
                     /locus_tag="VIV63_10325"
     CDS             complement(1479..>2363)
                     /locus_tag="VIV63_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6125041.1"
                     /translation="PGNLVAPLVPGDYEVRFSTAEKSLTVARAPIHIGPARNEPGKVA
                     VTLADSAKSGGAIEIILDASGSMLQKLGTERRIDIAKKTLTKLTSSTIPAGTPFAFRV
                     FGREVDSCQTDLDIPVGPLNPAAVGQRIAALVAKNGAKTPIGASLDKAADDLKSVQGE
                     KLIVLVTDGEETCGGDPAAAIERLRKAGVSTRVSIVGFALDDEKLASTFRRWSDAGGG
                     AFFDAKDAAGLDKSLTEAMRPGFEVVNAQGQVIASGIVGGEAVQAPAGNHTVRIKGRA
                     GSAKPAVVKPKETANVAL"
BASE COUNT          419 a          729 c          804 g          411 t
ORIGIN      
        1 gatcgcgggc atgaacgccg acacgctgtc gtactggcgg cgcaacgtgg atttccccaa
       61 tcaaactacc gccgaccagt tcttcgacga ggaacagctc gaagcctatc gcgagctcgg
      121 catggccatt gcgcagaccg ccgtgcgggc gctcaagggc gaacacgaac cctgggacga
      181 acccatgacc gcgctgcgcc gcaccttcgg ctggtagact tcgccccgct tggggtgggt
      241 tcacgagggg ttacacatga agaagctttt tatcggcctg tgcctggcgc tggccgttcc
      301 ggcggcgtac gcgcagaagt tcgagggcct ggcgaagaca ccgcagatgg gctggaacag
      361 ctggaacaag ttcgcctgca atgtgaacga gcagatgatc cgcgagacgg ccgacgcgat
      421 ggtttcgagc ggcatgaaag acgccggcta cctctacgtg aacatcgacg actgctggca
      481 cggcgaacgt gacgcccgcg gcttcatcca gccgcatccc gaacattttc cttcgggcat
      541 gaaggcgctc gcggattacg tgcattcgaa gggactgaag ctcggcatct actcggacgc
      601 gggctggaaa acctgcggcg gccggccggg cagccgcggc tacgaatacc aggacgcgtt
      661 gacgtacgcc gagtggggca tcgactacct caagtacgac tggtgcgaca cggcgggcct
      721 caaggccgaa ggcgcctatc tcacgatgcg tgaagccctg cgcaaggcgg gccggcccgt
      781 gctgttctcg ctgtgcgagt ggggcgacaa ccagccctgg gactgggcaa agcccgtcgg
      841 tcactcgtgg cgcaccaccg gcgacatctg ggactgcttc gactgcgtga aggatcacgg
      901 cacgtggaag tcccgcggcg tgttgcagat cctggatctc caggaaggcc ttcgcgtgca
      961 tgcgggtccg gaccactgga acgatgccga catgatggaa gtcggcaatg gcggcatgac
     1021 cgtcaacgag gatcgcgcgc atttttccat ctgggcgatg ctggcctcgc cgctgatcgc
     1081 cggtaacgac atccgcaaca tgtcgaaaga gacggccgcc attctcacca acaaggctgt
     1141 catcgcgctc aatcaggatg tgctgggtgt ccaggcgttc aagttcgcgt cgcgggacgg
     1201 tgtggagtac tggttcaagc cgctgcagaa cggggactgg ggcttcatgg cgctgaaccg
     1261 caatgccgac gcgcgcaaga tcacgttcga ctggaaggcc gaaaaagtga acgacgacct
     1321 gtcgaagcgc gatgccgcgt ttggcaagac gcgctactcg ttacgcaacc tgtggacggg
     1381 gcgagccgcc ggtcatacgg gcaaagcgct ggtggccgat ctgcccgggc atgacgtgct
     1441 ggtgctgcgg ctggccgccc ccggtagata gaacggcgct agagcgccac gttcgcggtc
     1501 tctttcggct tcaccaccgc gggtttcgcg gaacccgcgc gtcccttgat gcgcacggtg
     1561 tggttgcccg cgggcgcctg cacggcctcg ccgccgacga tgccgctggc gatgacctgg
     1621 ccctgcgcgt ttaccacttc gaagcctgga cgcattgcct cggtcaacga cttgtcgagg
     1681 ccggcagcgt ccttggcgtc gaagaatgcg ccgccaccgg cgtcggacca gcggcggaac
     1741 gtggaggcca gcttttcgtc gtcgagcgcg aatccgacga tgctcacgcg cgtactgacg
     1801 ccggccttgc gcagacgttc gatggccgcg gcgggatcgc cgccgcaggt ttcttcgcca
     1861 tcggtgacca gcacgatgag tttctcgccc tggacgctct tgaggtcgtc cgcggccttg
     1921 tcgagcgacg cgccgatggg agtcttcgcg ccattcttgg cgacgagcgc ggcgatgcgc
     1981 tgacccacgg ccgcgggatt cagcggtccg accgggatat cgagatcggt ctggcaggaa
     2041 tcgacctcgc gcccgaatac gcggaaggcg aacggcgtgc cggccggaat ggtggaggag
     2101 gtgagcttgg tcagcgtctt cttggcgata tcgatgcggc gctcggtgcc taacttctgc
     2161 agcatgctgc cggaagcgtc gagaatgatc tcgatggcgc cgccggactt cgcgctgtcg
     2221 gccagcgtca ccgcgacttt gccgggttcg ttcctggccg gaccgatgtg gatgggcgcg
     2281 cgcgccacgg tgagcgattt ttcggcagtc gaaaagcgca cttcgtagtc gccggggaca
     2341 agcggggcca cgagattgcc ggg
//
LOCUS       DATDEU010000431         6035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_520466, whole genome shotgun sequence.
ACCESSION   DATDEU010000431 DATDEU010000000
VERSION     DATDEU010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520466"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VIV63_10330"
     CDS             <1..441
                     /locus_tag="VIV63_10330"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY6125042.1"
                     /translation="GFDERTIAKFNDLTRIETEEIRDFLVLHYKATERDDTPFWRHCQ
                     AIRAPDSLRNRWEMYQRSGNLVIVPGDLFKESSWFAVYTGQGVIPQTQHPFADIPDAA
                     EVARRLEIIAGDVRKRADTFPSHDEYIRRHCAAPPLPAMPGKPM"
     gene            438..992
                     /locus_tag="VIV63_10335"
     CDS             438..992
                     /locus_tag="VIV63_10335"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY6125043.1"
                     /translation="MSAPSRHVQIEGRDVFVFDDLVPAEETARYFAAISQASFTRTET
                     ARPDAAEYRHWVCEMPLENLPRTSLWQATEKAVAAVRPGESFKPYRVYTNYSSYGDVL
                     LTHVDALPAARELTALWFLCERWDTEWGGETLFYDAAGEAQIAVSPRPARLLMFDGAI
                     RHAGKPPNRNCPVSRYTFAVKLRS"
     gene            complement(1004..1492)
                     /locus_tag="VIV63_10340"
     CDS             complement(1004..1492)
                     /locus_tag="VIV63_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY6125044.1"
                     /translation="MPSFDAVSELDTHELTNAVDQANRELQQRFDFKDTGAVFELNEK
                     DMKVSLKAPAEFQLKQMVDILAQRLSKRGIDVRCMEKKDAVSNLAEARQDILLRHGID
                     ADAGRKLQKLVKESKLKVQAAIQGDKLRVTGKSRDDLQAAMALLRKAELDRPVQFNNF
                     RD"
     gene            complement(1509..1895)
                     /locus_tag="VIV63_10345"
     CDS             complement(1509..1895)
                     /locus_tag="VIV63_10345"
                     /inference="COORDINATES: protein motif:HMM:NF022526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HEY6125045.1"
                     /translation="MKELIDNLTKTLGISGTQAEGGAAVLLKAAKEKLGAAAFDSQLG
                     SVPGLSDLLKKAPAAGGGGLGGLLGGLAGAMGGNAGLIASIVGGFGKLGLKPEDAKKF
                     VPVILGFLRTKVGPDVVSNLEKTLRA"
     gene            complement(1918..4323)
                     /locus_tag="VIV63_10350"
     CDS             complement(1918..4323)
                     /locus_tag="VIV63_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125046.1"
                     /translation="MPTDYEKLGAFYLGREFDPAANALKDDLVLYDSKDLTTHAVCVG
                     MTGSGKTGLCLSLLEEAAIDGVPAICIDPKGDLGNLMLTFPNLAPADFEPWVDAGDAA
                     RKGVSVGDLAAKTAETWKNGLAEWDQAPERIEKLRAAADIAIYTPGAETGLPLSVLRS
                     FSPPSPELLADASALRDRVGAVVSGLLSLLGIEADPIGSREHILLANILEGAWRAGLS
                     LDMTGLIQAVQKPAFDKLGAFDLETFFPAKDRLKLAMQLNNLIASPGFATWMQGEPLD
                     AQRLLFTPEGKPRISIISIAHLNDAERMFIVTLVLNEVIAWMRNQSGTGSLRAILYMD
                     EIFGFFPPTANPPSKQPMLTLLKQARAFGLGCVLATQNPVDLDYKGLANCGTWFIGRL
                     QTERDKMRVIEGLKSALPGAEDSADLEKLMSSLTQRVFLMRNVHDDAPVLMKTRWALS
                     YLRGPLTGPEISRVMASRKGAIAPASYPADGAAAAPATSRPAVGAGIPEYFLSPTKGT
                     GTVLYKPMVAGFAKLHYVDSKLALDDWQTAGWLAPFDDGGGNASWEDAREDAQLKSRL
                     VTSPAEGAEYGELPGPALRAASYSGWAKQLQSRLYETARANVLWCEAFKLASKAGETE
                     GDFRTRITLLAREKRDAAVADLRKRWQVKLQQLQDQIRRANERRERESSQLSQQKINT
                     ALSIGSSILGALLGRKAISATNVGRVGTAARSASRIGRESQDVERAEESLEVLQQRLE
                     ATKREVDTEVARLETTLDASTISLRAIEVPARKSDIAIGEVALVWTPWRKGADGFPAP
                     AYD"
     gene            complement(4394..4837)
                     /locus_tag="VIV63_10355"
     CDS             complement(4394..4837)
                     /locus_tag="VIV63_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6125047.1"
                     /translation="MTRRLISSGSTFEKSIGYSRAVVEGDWIFVSGTTGFDYSDMTIQ
                     DDAVAQCEQALKNIEAALKQANARFADVVRVHYLMTDAADFERCWPAMGKAFGEVRPA
                     ATMMVVGLADPRMKIEIEVTALLRKKAGRKAAPGKKKAKAAKRKK"
     gene            4901..5440
                     /locus_tag="VIV63_10360"
     CDS             4901..5440
                     /locus_tag="VIV63_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125048.1"
                     /translation="MKSFVVALALAAFAATAVGAEETRSVALVRAMRSDEIAVATAKL
                     AFLNGSMRERYGKTNNACVKRIAYTDFTAGWARVVESVLSPTEIETSLGFFQSDAGVK
                     YVEGLLRRLRARQGEASVLPEIKGKEEITPPQLARISEFSSSDLGRKVMGKDLNLSPA
                     AEAMGNQANEQIAAKCGGK"
     gene            complement(5444..5848)
                     /locus_tag="VIV63_10365"
     CDS             complement(5444..5848)
                     /locus_tag="VIV63_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125049.1"
                     /translation="MSELEGLIPHAGSMCLLEHVVAHSAQDIECTATSHTSPENPLRR
                     DGQLSAVHLVEYAAQAMAAHGALAAGGVQRGMLASLRDIRLHVGRIDDIGTPLDIKAT
                     RRIRQVTGSLYDFSIRSSGRLLCEGRIAIAFV"
     gene            complement(5845..>6035)
                     /locus_tag="VIV63_10370"
     CDS             complement(5845..>6035)
                     /locus_tag="VIV63_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125050.1"
                     /translation="LACATTVDEEMRIEEPALEQLRLGNPATRALPLLRALAMRQFGQ
                     VVLPMTPGANLAIDVRAP"
BASE COUNT          998 a         1937 c         1982 g         1118 t
ORIGIN      
        1 gggttcgatg aaaggaccat cgccaagttc aacgacctca cgcgcatcga gaccgaagag
       61 atccgcgatt tcctcgtgct gcactacaag gccaccgagc gtgacgacac gccgttctgg
      121 cggcactgcc aggcgatccg cgcgccggat tcgctgcgca atcgctggga gatgtaccag
      181 cgcagcggca atctcgtgat cgtgccgggc gatctgttca aggagtcgag ctggttcgcc
      241 gtgtacacgg ggcagggcgt cattccgcag acacaacatc cgttcgccga cattcccgat
      301 gccgccgaag tcgcgcgccg cctcgaaatc atcgccggtg atgtccgcaa gcgcgcggac
      361 acgttcccct cgcacgacga atacatccgc cggcattgcg ccgcgccgcc tctgccggcc
      421 atgcccggga aaccgatgtg agcgctccga gccggcatgt gcagatcgaa ggacgcgacg
      481 tgttcgtgtt cgacgacctg gtgcccgccg aagagacggc gcgctacttc gcggcgatct
      541 cgcaggcctc gttcacgcgc acggaaaccg cgcgccccga tgccgccgaa taccgccact
      601 gggtttgcga gatgccgctc gagaacctgc cgcgtaccag cctctggcag gcgaccgaga
      661 aggccgtcgc ggccgtgcgg ccgggtgagt cattcaagcc ataccgcgtc tataccaact
      721 actcgtccta cggcgatgtg ctgctgacgc acgtcgacgc gttacccgcg gcgcgcgaac
      781 tcaccgcgct gtggttcctg tgcgaacgct gggatacgga gtggggcggc gagactctct
      841 tctatgatgc ggcgggcgag gcgcagattg cggtgtcgcc gcgtcccgcg cggttgctga
      901 tgttcgatgg cgccatccgc catgccggca agccgccgaa ccgcaactgc ccggtgtcgc
      961 gctacacctt cgccgtcaag ctgcgtagtt agtcgcggcc ggatcaatcc cggaagttgt
     1021 tgaactggac ggggcggtcg agctccgcct tgcgcagcag cgccatcgcg gcctgcagat
     1081 cgtcacgcga cttgcccgtc acgcgcagct tgtcaccctg gatcgccgcc tggaccttga
     1141 gttttgattc cttgacgagc ttctgcagct tgcggccggc atccgcgtcg atgccgtggc
     1201 gcaggaggat gtcctggcgc gcctcggcca ggttgctgac ggcgtccttc ttttccatgc
     1261 agcgcacgtc gatgccgcgt ttgctcaggc gttgcgcgag gatgtcgacc atctgcttga
     1321 gctgaaattc cgccggcgcc ttcaggctga ccttcatgtc cttttcattg agctcgaaca
     1381 cggcgcccgt gtccttgaag tcgaaacgct gctggagctc gcgattcgcc tggtcgacgg
     1441 cattggtgag ttcgtgggtg tcgagttcag aaacagcatc gaaagacggc atgggagatt
     1501 ccgggagttc aggcgcgaag ggttttctcg aggttggaca cgacgtccgg gccgaccttg
     1561 gtgcgcagga aacccaggat caccgggacg aacttcttgg cgtcctcggg cttgagtccc
     1621 agcttgccga agccgccgac gatcgaggcg atgaggccgg cgttgccgcc catcgcgccg
     1681 gcgagtccgc ctaacaaacc acccagaccg ccgccgcccg cggcgggcgc tttcttcagg
     1741 aggtcgctga gcccgggcac gctgccgagc tgactgtcga atgcagcggc gccgagcttt
     1801 tccttcgcgg ccttcagcag gactgccgcg ccgccctcgg cctgggtgcc gctgatgccg
     1861 agagtcttgg tcaggttgtc gatgagctct ttcatggcgg gttcctcgga gtgggcgtca
     1921 gtcgtaggcc ggggcgggga aaccatcggc gcccttgcgc cagggagtcc acaccagggc
     1981 gacctcgccg atcgcgatat cggacttgcg cgccggcact tcgatggcgc gcagggaaat
     2041 ggtggaggca tccagcgtgg tctcgagccg cgcgacttcc gtgtcgactt cgcgcttcgt
     2101 ggcctcgagc cgctgctgca ggacttcgag gctttcctcg gcgcgctcca cgtcctgcga
     2161 ttcgcggccg atgcgcgagg ccgaacgcgc cgcggttccc acgcgcccga cgttcgtggc
     2221 ggagatggcc ttgcggccta acaacgcgcc gagtatcgag ctgccgatgg acaacgcggt
     2281 gttgatcttc tgctgcgaaa gctggctcga ctcgcgttcg cggcgctcgt tggcgcggcg
     2341 gatctggtcc tggagctgct gcagtttcac ctgccagcgc ttgcgcaggt ccgccacggc
     2401 ggcatcgcgt ttctcgcgtg cgagcagcgt gatgcgcgtg cggaaatcgc cttcggtctc
     2461 gccggccttc gacgccagct tgaacgcttc gcaccacagg acgttcgcgc gtgccgtttc
     2521 atacagccgc gactgcagct gcttcgccca gcccgagtag ctcgcggcgc gaagtgcggg
     2581 cccgggcagc tcgccatact cggcgccttc tgccggtgac gtaaccagtc gcgatttgag
     2641 ctgcgcgtcc tcgcgggcgt cttcccacga cgcatttccg ccgccgtcat cgaagggcgc
     2701 gagccaaccg gcggtctgcc aatcatcgag cgcgagcttg gaatcgacgt agtgcagctt
     2761 ggcgaatccg gcgaccatgg gcttgtagag caccgtgccc gtgcccttcg tcggactcag
     2821 gaaatattcc gggattccgg cgcccactgc cgggcgcgaa gtggccgggg cggccgcggc
     2881 gccgtccgcg gggtagctag cgggggcgat tgcgcctttt cgcgaagcca tgacgcgcga
     2941 tatttccggc cccgtgagcg gccctcgcag ataggagagc gcccatcgcg tcttcatgag
     3001 caccggcgcg tcgtcgtgaa cgttgcgcat gaggaacacg cgctgtgtga gactcgacat
     3061 gagcttttcg agatcggcgc tgtcctcggc accgggtaat gccgacttca atccttcgat
     3121 cacgcgcatc ttgtcgcgct cggtctgcag ccggccgatg aaccaagtgc cgcagttggc
     3181 caggcccttg taatcgagat cgacgggatt ctgcgtcgcc agcacgcaac ccaggccaaa
     3241 cgcgcgcgcc tgcttgagca gcgtgagcat gggttgtttg gacggcggat tcgcggtggg
     3301 cggaaagaat ccgaagatct cgtccatgta gaggatggcg cgcaaggatc cggtgcccga
     3361 ctggttgcgc atccacgcga tgacttcgtt gagcaccagc gtgacgatga acatgcgttc
     3421 ggcgtcgttg agatgcgcga tcgagatgat cgagatgcgc ggcttgcctt cgggcgtgaa
     3481 cagcaggcgc tgcgcatcga gcggctcgcc ctgcatccag gtggcgaagc ccggcgaggc
     3541 gatgaggttg ttgagctgca tcgcgagctt gagacggtct ttcgcgggaa agaacgtttc
     3601 gaggtcgaat gcgcccagct tgtcgaaggc cggtttctgc acggcctgga tcaggcccgt
     3661 catgtcgagc gagagacccg cgcgccaggc gccttcgaga atgttggcga gcaggatgtg
     3721 ttcgcgggag ccgatggggt ccgcttcgat accgagcagg ctgagcaatc ccgagacgac
     3781 ggcgcccacg cggtcgcgca acgcgctggc gtcggctaac aactcgggcg agggcggtga
     3841 aaacgaccgc agcacggaca gcggcagtcc cgtttcggcg cccggcgtgt agatggcgat
     3901 gtccgcggcg gcgcgcagtt tttcgatgcg ctctggcgcc tggtcccatt ccgccaggcc
     3961 gttcttccag gtctcggcgg ttttcgcggc gagatcaccc acactcacgc ctttgcgtgc
     4021 cgcgtctccc gcgtccaccc agggttcgaa atccgcgggc gcgaggtttg gaaacgtcag
     4081 catcagattg ccgaggtcgc ccttggggtc gatgcagatg gccggcacgc cgtcgatggc
     4141 ggcctcttcg agcagcgaga gacacaatcc ggtcttgccg ctgccggtca tgccgacgca
     4201 caccgcgtga gtagtcaggt ccttggagtc gtagaggacc aggtcgtcct tgagcgcgtt
     4261 ggcggccggg tcgaattcgc gaccgaggta gaaggcgcct agcttttcgt aatctgtggg
     4321 catttcattc ctgggtcgcg ggccggagtg gggtcaggca ccgttttccg gacggaaaac
     4381 ggagccagac cccttacttc ttgcgcttcg cggctttggc cttcttcttt cccggtgctg
     4441 ccttgcggcc cgccttcttg cgaaggaggg cggtgacttc gatctcgatc ttcatgcgcg
     4501 ggtccgcgag cccgaccacc atcatggtgg cggcgggacg cacttcgcca aaggccttgc
     4561 ccatggcggg ccagcaacgc tcgaagtccg cggcgtccgt catgaggtag tgcacgcgta
     4621 cgacatccgc aaaccgggcg ttcgcctgct tcagcgccgc ctcgatgttc ttgagcgcct
     4681 gctcgcactg cgccacggcg tcatcctgga tggtcatgtc ggaataatcg aagccggtgg
     4741 tgccggaaac gaagatccaa tcgccttcga ccacggcgcg cgaatagcca atgctcttct
     4801 cgaaagtcga tcctgaagaa atcaatcggc gtgtcatgca ggctcctcgc ggtatattcc
     4861 gcaatcggat tctgccacgg ttcgtcaccc ggaaaaccgc atgaaatcat ttgtagttgc
     4921 actcgcactg gccgcattcg cggcgaccgc ggttggcgcc gaagaaacgc ggtccgtcgc
     4981 gctggtgcgc gcgatgcggt ccgacgagat agccgtcgcc acggccaaac tcgcctttct
     5041 caacggctcc atgcgcgagc gctacgggaa gaccaacaat gcctgcgtga agcgcattgc
     5101 ctacaccgac ttcaccgccg gctgggcgcg agtcgtcgag agcgtgctga gcccgaccga
     5161 aatcgaaacc tcgctcggct tctttcagtc cgacgccggc gtcaaatacg tggaaggatt
     5221 gctgcgccgc cttcgggccc gacaggggga agcctcggtg ctgcccgaga tcaagggcaa
     5281 ggaagaaatc acgccgccgc agctcgccag gatctccgaa ttctcgagca gcgatcttgg
     5341 ccgcaaggtc atgggcaagg atctgaatct ttctcccgcc gccgaagcga tgggaaatca
     5401 ggccaacgag cagatcgccg ccaagtgcgg cggcaagtag cgttcacacg aacgcgatgg
     5461 cgattcggcc ttcgcacagc agccggccac tgcttcggat actgaagtcg taaagcgatc
     5521 cggtgacctg cctgatgcgc cgcgtggcct tgatgtcgag tggcgtgccg atgtcgtcga
     5581 tgcgccccac gtgcaaacgg atgtcgcgca gcgacgccag catgccgcgc tgaacgccac
     5641 cggctgccag ggcgccatgc gccgccatgg cctgcgccgc gtattcgacc aggtgcactg
     5701 ccgagagttg gccgtcgcgg cgaagtggat tttcgggcga cgtatgcgag gtcgccgtgc
     5761 attcgatgtc ctgcgccgag tgcgccacga cgtgctccag caggcacatg ctgccggcgt
     5821 gagggatgag accttcgagc tcgctcatgg tgcgcgcaca tcgatggcca ggttggcgcc
     5881 cggtgtcatc ggcaagacca cctggccgaa ctgccgcatc gccagcgctc gcagcaacgg
     5941 aagagcgcga gttgccggat tgccgagccg caactgctcg agcgccggtt cctcgattct
     6001 catttcttca tcgacggtcg tagcgcaggc cagcg
//
LOCUS       DATDEU010000432         2519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2124268, whole genome shotgun sequence.
ACCESSION   DATDEU010000432 DATDEU010000000
VERSION     DATDEU010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124268"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VIV63_10375"
     CDS             <1..520
                     /locus_tag="VIV63_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6125051.1"
                     /translation="AAAALAANPAYVGQTRTTCVATMLQAGHAENALPQSAVANVNCR
                     IFPGVKIEDVRLALAGVVGEGVDVAVDGEPMSSDASPLRNDIVAAVTRVVHKFYPTAN
                     IVPQQASGATDGLVFRAVGIPTYGVDPTFIKDKDAFAHGLNERIPVKSFYDGLEMWYL
                     LVKDLAGTGKKK"
     gene            538..2457
                     /locus_tag="VIV63_10380"
     CDS             538..2457
                     /locus_tag="VIV63_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY6125052.1"
                     /translation="MPGDIAQPAGTLEVALAHASRLLQTDANAALEQAGEILKVIPDQ
                     PQALTLEGLALGRLGRGDEAIERLRRAVHLRPTQPEAWRALGDHYTALEMRDAADGAY
                     AESIRHSTHDPKLMGAAMALVENRIPEAEAVLREHLKRHPTDVAAIRMLAEVAGRIGR
                     YLDAENLLARCLELAPGFAAARHNYAMVLHRQNKPEAALAQVELLLAEEPRNPGYRNL
                     KAAILGRIGEYSASIEHYRSVLADYPNQAKVWMSLGHALKTAGNNAESVEAYLKSIER
                     APDLGEAYWSLANLKTFRFSPQQTQAMREQLAGGKLSPDDRFHFEFSLGKALEDAGEY
                     GESFAHYLEGNRLRREMIRYDADENAAHVERSKKLFTREFFAERGGVGCEAPDPIFVV
                     GLPRSGSTLIEQILASHSQVEGTMELPDVAMLARVVGGRTTRTAGSAYPRALEKFSAD
                     ELRALGERYLAQTRIQRKTPAPIFIDKMPNNFAHVGLIHLMLPKAKIVDARRHPLGCC
                     FSGFKQHFARGQNFTYDLAEIGRYYRDYVELMAHFDAVLPGRVHRVLYENMVEDTETE
                     VRRLLDYCGLPFEEGVLRFHENQRAVRTASSEQVRQPIFKEGVDHWRHFEPWLAPLKE
                     ALGDVVSAYPSTPQF"
BASE COUNT          455 a          851 c          819 g          394 t
ORIGIN      
        1 ggctgccgcc gcgctcgccg cgaatcccgc gtacgtgggc cagacgcgca ccacctgcgt
       61 ggccacgatg ctgcaggccg ggcacgccga gaacgcgtta ccgcaaagcg ccgtcgccaa
      121 cgtgaattgc cgcatcttcc cgggcgtgaa gatcgaggac gtgcgtctcg cgctggccgg
      181 agtcgtgggc gagggcgtcg acgtcgcggt ggatggcgag ccgatgtcga gcgacgcatc
      241 gccgctgcgc aacgacatcg tcgcagccgt gacgcgcgtc gtgcacaagt tctatccgac
      301 ggcgaacatc gtgccccagc aggcttcggg cgccaccgat ggcctcgtgt tccgcgccgt
      361 gggcattccg acctatggcg tggacccgac cttcatcaag gacaaggacg cgttcgcgca
      421 cgggctcaac gagcgcattc cggtgaagtc gttctacgac ggcctcgaga tgtggtacct
      481 gctcgtcaag gatctggccg gtaccggcaa gaaaaagtga tcgcaggtag ctagtccatg
      541 cccggggaca tcgcgcagcc ggccggcacg ctcgaggtgg cgctcgcgca cgcgtcgcgc
      601 ctgctgcaga ccgacgcgaa cgcggcgctc gaacaggccg gcgagatact caaggtcatt
      661 cccgaccagc cgcaggcgct cacgctggaa ggcctggcgc tggggcgcct gggccgtggc
      721 gatgaagcca tcgaacgcct gcgccgcgcg gtgcatctgc ggcccacgca accggaggcg
      781 tggcgcgcgc tcggcgatca ctacaccgcg ctcgagatgc gcgacgccgc cgacggtgcc
      841 tacgccgagt ctatccgcca ttccacgcac gacccgaaac tcatgggcgc cgcgatggcg
      901 ctggtcgaga accgcattcc cgaagccgaa gcggtgctcc gggagcacct gaagcgacat
      961 cccacggatg tggcggcgat ccgcatgctg gccgaagtgg cggggcgcat cggccgctac
     1021 ctcgacgcgg agaatctgct ggcgcgttgc ctcgagctcg cgccgggttt cgcagccgcc
     1081 cgccacaact acgccatggt gctgcaccgg cagaacaaac ccgaggccgc gctcgcgcag
     1141 gtggagctgc tgctcgccga agagccgcgt aatcccggct accgcaatct caaggccgcc
     1201 atcctgggac gtattggcga gtactcggcc tccatcgaac actatagaag tgtgctggcc
     1261 gactacccca accaggccaa ggtgtggatg agtctcggtc acgcgctcaa gaccgcgggc
     1321 aacaacgcgg aaagcgtcga ggcctatctc aagagcatcg agcgcgcgcc cgacctcggc
     1381 gaggcgtact ggagtctcgc caatctcaag accttccggt tttcgccgca gcagacgcag
     1441 gcgatgcgcg agcagctcgc gggcggcaag ttgtcgcccg acgatcgttt ccacttcgaa
     1501 ttctctctcg gcaaggcgct ggaagacgcc ggcgaatacg gggagtcgtt cgcgcactac
     1561 ctcgaaggca accggctgcg ccgggagatg atccgttacg acgccgatga gaacgccgcg
     1621 catgtcgagc gatcgaaaaa gctcttcacg cgcgaattct tcgcggagcg cggcggcgtg
     1681 ggctgtgagg cgcccgaccc gatcttcgtg gtgggcctgc cgcgctcggg ctcgacgctc
     1741 atcgagcaga tcctcgccag ccattcgcag gtcgagggca ccatggaact gcccgatgtc
     1801 gcgatgctgg cgcgcgtggt cggcggtcgc acgacgcgca cggcgggcag cgcctatccg
     1861 cgcgcgctcg agaaattctc cgcggacgaa ctccgcgcgc tgggcgaacg ttaccttgcg
     1921 cagacgcgca tccagcgcaa gacgccggcg ccgatcttca tcgacaagat gcctaacaac
     1981 ttcgcgcacg tggggctcat ccacctgatg ttgccgaagg cgaagatcgt cgacgcgcgc
     2041 cggcatccgc tcggctgctg cttctcgggc ttcaagcagc acttcgcgcg aggccagaac
     2101 ttcacgtacg accttgccga gatcggccgc tactaccgcg actacgtcga actcatggcg
     2161 cacttcgatg cggtgttgcc gggccgcgtt catcgcgtgc tctacgaaaa catggtcgag
     2221 gacaccgaaa ccgaggtgcg caggcttctc gactactgcg gcctgccgtt cgaggaaggc
     2281 gtccttcgat tccatgaaaa tcagcgcgcg gtgcggaccg cgagctcgga acaggtacgc
     2341 cagcccatct tcaaagaggg tgtggaccac tggcggcact tcgagccctg gctcgctccg
     2401 ctcaaggagg cgctcggcga cgtcgtttcg gcgtatccct ccacgccgca gttttgaatt
     2461 cgtggcggct ggcatacact ccagccacaa caacacgggg atcactcatg acacgcagc
//
LOCUS       DATDEU010000433         3577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1978602, whole genome shotgun sequence.
ACCESSION   DATDEU010000433 DATDEU010000000
VERSION     DATDEU010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978602"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="VIV63_10385"
     CDS             <1..1552
                     /locus_tag="VIV63_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEY6125053.1"
                     /translation="CEAAIARFDLPREFSPEAVREAESYGNEVDPAEAARRVDLRNLP
                     LVTIDGEDAKDFDDAVYCEPHAKGFRLIVAIADVSYYVRRGTALDASARERGTSVYFP
                     TRVIPMLPFALSNVLCSLQPNVDRLCFASEMIVSRTGVLMESRCYTAVMRSVRRLTYD
                     QAFAALFEKRPEALADMAGLADKLLPLVDVYRALLKARHKRGALEFDAAEAEFDFDGS
                     GRIQRVYMYQRNEAHKLIEECMILANVAVARELELRRIGTLFRVHGKPEEKKLNLLLE
                     TLNALGVAAEFPEEVSPRDFRAITDRLGHDESRPFIESLIVRSMMQAVYQPTNIGHFG
                     LALKHYAHFTSPIRRYPDLVVHRCLRAMLSDSDPYGMRYDGGQLALAGAELTALEKRA
                     DESDRYVNSWLKCVYLRDRIGQTFEGLITTVVEFGAFVQLTAVGTDGLLHIDSLRDDE
                     YVMEPGGRAWVGRTSKRRLSLGARVHVIVTSVNPIEGLVDLSLVTVETPGKIGPQKSP
                     HRKKGGRA"
     gene            1651..2148
                     /locus_tag="VIV63_10390"
     CDS             1651..2148
                     /locus_tag="VIV63_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125054.1"
                     /translation="MIQSMRIRLPAIFVGVASLLASPGGAVHASDTAAQTVDITVQAV
                     NEIALGTAVTLNVNSAAAGINPTGLASSTYSITTNSTASKKITAQLLAAPTSGLTLSV
                     NLQAPSTGVSAGSVPLGVAPVDAVNSIEAVTASGLTISYAAAATVAVAPTSYSAEVTY
                     TIVND"
     gene            2265..2696
                     /locus_tag="VIV63_10395"
     CDS             2265..2696
                     /locus_tag="VIV63_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125055.1"
                     /translation="MSAAHGADVSVLGGPWFMSIGAAHLEAGAGTDFRSPIASDVFIA
                     VLAITNTGGASWNLSIAREGNEMQWPAGVSIALRRSGGSAEAGIVDGLAFRTLTSDLQ
                     PFFSGNGDYASVEILLRLDGVSRQTPPGFYSLAIRYAVDAP"
     gene            2693..3487
                     /locus_tag="VIV63_10400"
     CDS             2693..3487
                     /locus_tag="VIV63_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125056.1"
                     /translation="MKRRIASAAIALLACWPAAFAGITPEGEATQIHSAKPGDTYRGA
                     IAIRNPGSEVVQVKLYQSDYLFFADGQNRFDRPGSSTRSNAGWLRLNREQIDIAPGGV
                     ATVDYEVQVPPDSRLTGTYWSVVMVQELPAGEAAGATRPGMKLTQTLRHAIQIITEMG
                     ESGRSEIAFRNARLVGEGAERGLDIDLENTGERWLRTDVWLELHDTNGRVVGRFVAPR
                     RRSFPGTSVRNRIELGSAPAGKYLGLLVADGGRNDLFGMQVELDLR"
BASE COUNT          614 a         1145 c         1186 g          632 t
ORIGIN      
        1 ttgtgaagcg gccatcgcgc gcttcgatct tccgcgcgag ttctcgcccg aagcggtccg
       61 cgaagcggag agctacggaa acgaagtcga tcccgcggaa gcggcgcggc gcgtggatct
      121 gcgcaacctg cctctcgtga cgatcgacgg cgaagacgcc aaggatttcg acgacgcggt
      181 gtattgcgaa ccgcacgcca agggtttccg gctcatcgtg gccatcgccg atgtgagcta
      241 ctacgtccgg cgtggcacgg cgctcgacgc cagcgcgcgt gagcgtggca cgagcgtgta
      301 tttccccacg cgcgtcattc ccatgctgcc gttcgcgttg tcgaacgtgt tgtgctcgct
      361 gcagccgaac gtcgatcgcc tctgcttcgc atcggagatg atcgtctcgc gcacgggtgt
      421 gttgatggaa tcgcgctgtt acacggcagt gatgcgatcc gtccgacgtc tgacttacga
      481 ccaggctttc gccgcgctgt tcgagaaacg gcccgaggcg ctggcggaca tggcggggct
      541 cgctgacaaa ttgctgcccc tcgtcgacgt ctatcgcgcg ctgctcaaag cgcgacacaa
      601 acgcggtgcg ctcgagttcg acgccgccga ggccgagttc gacttcgacg gctcagggcg
      661 catccagcgt gtgtacatgt atcagcgcaa cgaggcgcac aagctcatcg aagagtgcat
      721 gatcctcgcc aacgtggccg tggcgcgcga gctcgagttg cggcgcatcg gcacgctgtt
      781 ccgcgtgcac ggcaaaccgg aagagaagaa gctcaaccta ctgctggaaa cgctcaatgc
      841 gcttggcgtc gccgcggagt tcccggaaga ggtgagtccg cgtgatttcc gcgccatcac
      901 cgaccggctg ggtcacgacg aatccaggcc gttcatcgag tcgctgatcg tgcgttcgat
      961 gatgcaggcg gtgtatcaac cgacgaacat cgggcacttc ggcctcgcgc tcaaacacta
     1021 cgcgcacttc acgtcgccca ttcgacgcta tccggatctg gtcgtgcatc gctgcctgcg
     1081 cgcgatgttg tcggactccg atccgtacgg catgcgctac gacggtggac agctcgcgct
     1141 ggccggcgcc gagctcacgg cgctcgagaa acgcgccgac gaatcggatc gttacgtgaa
     1201 ttcctggttg aagtgtgtgt acctgcgcga ccgcatcggc cagacgttcg aaggtctcat
     1261 caccacggtg gtggaattcg gcgcgttcgt gcagctcacg gccgtgggta cggacggact
     1321 gctgcacatc gacagtctgc gtgatgacga atacgtcatg gaaccgggcg gccgcgcgtg
     1381 ggtgggccgg accagcaagc gccggctgtc gctcggtgcg cgcgtgcacg tcatcgtgac
     1441 cagcgtgaat ccgatcgagg gactcgtcga cctctcgctg gtgacggtgg aaacgccggg
     1501 caagattggt ccgcagaagt cgccgcaccg gaagaagggc gggcgcgcat aacggcgggc
     1561 aggcaatcga atccgttttg gctgcttcgc gggtggcggc ggattctcgg tggatagact
     1621 ggtgtggccc cgtagtcagg agccataaaa atgatccagt ccatgcgaat tcgccttccc
     1681 gcaatcttcg tgggagtcgc ctcgttgctt gcgtcacccg gcggtgcggt ccacgcctcc
     1741 gataccgccg cccagaccgt cgacatcacc gtgcaggcgg tcaatgagat cgcgctgggc
     1801 accgccgtca cgttgaacgt caattccgca gcggccggca tcaaccccac cggtctcgct
     1861 tcatccacct actcgatcac gacaaactcg acggccagca agaagatcac ggcccaactg
     1921 ttggccgcgc cgaccagcgg actgacgctc agcgtgaacc tgcaggcgcc gagcaccggc
     1981 gtttccgccg gctccgtgcc gctgggtgtt gcgccggtcg atgccgtgaa cagtatcgaa
     2041 gccgtgaccg cgagcggact cacgatcagt tatgcggcgg ccgccacggt cgcggtcgcg
     2101 ccgaccagct actccgcgga agtgacttac acgatcgtca acgactgagc ggcagcgtgc
     2161 gcacgtcatc tgccctggcg ccgcgggagg ccggcgccag atctcgcacc gcggccgtgc
     2221 gcgtcgggca agtcttccgt cgcagtctgc tcctcgtctt ctgcatgtcc gcggcgcacg
     2281 gtgcggatgt gtcggtgctc ggagggccgt ggttcatgtc catcggcgcc gcgcatctgg
     2341 aggcgggggc gggcacggat ttccgttccc ccatcgcttc cgacgttttc atcgcggtgc
     2401 tcgccatcac caacaccgga ggagcgagct ggaatctcag catcgcgcgc gagggcaacg
     2461 agatgcagtg gccggccggc gtgagcatcg cactgcgacg cagcggcggc agcgcggagg
     2521 cgggcatcgt cgatggcctc gcgttccgca cgttgaccag cgacctgcag ccgttcttca
     2581 gcggtaacgg cgactatgcg agcgtcgaaa tcctgcttcg gctggacggc gtcagcaggc
     2641 agacgccgcc gggtttctat tcgctggcga tccgctacgc ggtcgacgcg ccgtgaagcg
     2701 acgaatcgct tccgccgcaa tcgcattgct cgcctgctgg ccagccgcgt tcgcggggat
     2761 cacgcccgaa ggcgaagcga cgcagattca cagtgcgaaa cccggcgata cctatcgcgg
     2821 agcaatcgcg atccgcaacc ccggctctga agtcgtgcag gtgaagctct accagtcgga
     2881 ttacctgttc tttgccgacg gccagaaccg tttcgacagg ccgggctcgt cgactcgttc
     2941 gaatgccggg tggctgcggc tcaaccgcga gcagatcgac attgctccgg gtggcgttgc
     3001 aaccgtcgac tatgaagtgc aggtgccgcc ggattccagg ctcaccggca cgtactggag
     3061 cgtggtcatg gtgcaggaac tgccggcggg agaagccgcg ggtgcgactc gccccggcat
     3121 gaaactgacg cagacacttc ggcacgccat ccagatcatc accgagatgg gcgagtcggg
     3181 gcgcagcgaa atcgcgtttc gcaatgcgcg gctcgtgggc gagggagcgg agcgcgggct
     3241 cgacatcgac ctcgagaaca ccggcgaacg ctggctgcgc accgatgtct ggctcgaatt
     3301 gcacgacacg aatggccgtg tcgtgggccg cttcgtggcg ccgcggcgcc gatcgtttcc
     3361 agggacctcc gtgcgcaacc ggatcgaact gggctcggcg cccgcgggaa aatatcttgg
     3421 gctgctcgtc gccgatggag gccggaatga ccttttcggc atgcaggtcg agctggatct
     3481 tcgctagcta cctgctcgcc gcgagcacgg ccgcgttcgc gcaggatggt gcgttgcgcg
     3541 tgcggcccgc ggatcgcgag cagatctcga gcgaacc
//
LOCUS       DATDEU010000434         6623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_227390, whole genome shotgun sequence.
ACCESSION   DATDEU010000434 DATDEU010000000
VERSION     DATDEU010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227390"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="lnt"
                     /locus_tag="VIV63_10405"
     CDS             <1..954
                     /gene="lnt"
                     /locus_tag="VIV63_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY6125057.1"
                     /translation="SRIVAGASFAAIWAVAFGLRGIEWTKPYSRPISVAVVQGAIPQD
                     EKWLTENLDAILELYKTRTREAHGADLIVWPESAIPDLANNHIEFYRDVYQEASAKGS
                     SLMVGTLRAEENAKTGEEEYFNSVLSMDKSTPGVGWYDKHHLVPFTEFVPVPGFVRQW
                     LRLMSLPYSDFNRGAAQQAPLESAGQRISASVCYEDAYGATQLPALRTATMLVNVTND
                     AWFGHSPARYQHLQISRMRSLEAGRPMVRAANDGVSAVIGHRGEIVAMAPEYEANVMR
                     ATVQPRIGLTPYAGTGNWAVVCLALVFALASAMLRRRTKTV"
     gene            1053..2546
                     /locus_tag="VIV63_10410"
     CDS             1053..2546
                     /locus_tag="VIV63_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY6125058.1"
                     /translation="MSLFVARRLGAVAIVAFAVGACSPQSSIAAAQAEVPPSTVAAAA
                     QAPTSPQPVGTLNGRVLPDFATLVEQVGPAVVNVSVLEKPHRANKQSSEDADDPVQEF
                     FRRFGMPNGPQGRGFEQQPRQGEGSGFIVSADGYILTNAHVVADADEVTVRTTDRREY
                     TAKVVGLDKRTDVAVLKIEGKDLPVVRLGDPSKLRPGEWVLAIGSPFTFENSVTAGIV
                     SATGRSMPGEDLVPFIQTDVAVNPGNSGGPLFNLNGEVVGINSQIYSRSGGYMGLSFA
                     IPIDVANNVRQQLVSTGKVTRGRIGVQIQEVNAQFADAFGLDRPRGALVGQVIEGGPA
                     EKAGVKTGDVILSVNGKNVERSAALPGVISAIKPGDTAKVEVWREGNSKTLAVKVEEF
                     AEETQKVANRDVEEPAKADKLGLSVRPLGADERKSASTEGYLLVEDVTGPAAQAGVRP
                     GDVILGVNGKAVKSIAELKNATASGSKTVAILLEREGNQLFLPIRIS"
     gene            complement(2609..3508)
                     /locus_tag="VIV63_10415"
     CDS             complement(2609..3508)
                     /locus_tag="VIV63_10415"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6125059.1"
                     /translation="MGECEKLPGTVERYQERFARVLAHIEANSETALSIDALSAIAAF
                     SRHHFHRQFSGFLGMSTYRYVQFMRMRRASWRLAFRPELSVNDIALATGYEGPEAFAR
                     AFRQYSGQSPSEFRDQPDWTEWHSRFRPFIQLRQTTMNTITSPPIVRVTTFPSTRVAR
                     LEHRGSVLRLGDSIRRFIEWRKANKLPPQISATFNIIHNDPDETPPEEFRFDLCAATD
                     RPIDTNESGVTEFVIPAGRCALLRHRGSDDQLGRSIMHLYANWLPTSGEELRDFPLFM
                     QRVKFFPDVPDGDAETDIYLPLK"
     gene            complement(3570..3860)
                     /locus_tag="VIV63_10420"
     CDS             complement(3570..3860)
                     /locus_tag="VIV63_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125060.1"
                     /translation="MSRSLFRNASAVIPLVMSGVALATVLIHVLTTHDAAATDEGTAA
                     HVFQLLFAGQAPFVAFFALKWLPRLPSEAMYVMAVQAGAALVALAPVVIFDL"
     gene            4044..>6623
                     /gene="leuS"
                     /locus_tag="VIV63_10425"
     CDS             4044..>6623
                     /gene="leuS"
                     /locus_tag="VIV63_10425"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY6125061.1"
                     /translation="MQERYEAPAVERAAQEFWDRERVFEAREDAGGEAGKFYCLSMFP
                     YPSGRLHMGHVRNYTIGDVLTRFAKMQGKSVLQPMGWDAFGLPAENAAIKNNVPPAKW
                     TRDNIAYMKAQLKSLGFAFDWTREIATCDPSYYRWNQWLFLRMLEKGIAYRKTGVVNW
                     DPVDQTVLANEQVIDGRGWRTGAIVEKREIPMYYLGITKYADELLGDLEKLPDWPERV
                     KTMQANWIGKSEGCEISFPFAPDTRALMAADGALKVFTTRADTLFGVSFMAIAAEHPI
                     ALAAGAKNPALQTFIDECRRGSTMEADIATAEKKGMRTGLHVLHPFTGQMVEVWVANY
                     VLMAYGEGAVMGVPAHDARDFEFALKNKLAVITVIHSKTGAYDKVVEPWIDAYTEYGI
                     TVNSGVFDGLDFQAAVDAIAVALEKKGLGRKRTQWRLRDWGISRQRYWGCPVPLIHCA
                     ACGVVPVPEKDLPVVLPEDLIPDGSGNPLAKHGAFLNVACPTCGRAARRETDTMDTFV
                     DSAWYYMRYCDPHLESAMVGAGTKYWMPMDQYIGGIEHAILHLLYARFWTKVMRDMKL
                     VAIDEPFTKLLTQGMVLNHVWARTSAQGGVDYFPPDEVTPVGEAEGRPSSGRLADGSA
                     VEYRGMLKMGKTERNGVDPQDLIDRYGADTARLFVMFAAPPEQSLDWNNAGVEGANRF
                     LKRLWKFAARHADTIDCAAGRQPARGGAPALRHEIHSVLRQVSYDYERMQYNTVVSGA
                     MKLLNALETHAGQGSVAPAPLREGFGILLRVLYPVCPHTTWTLWNELGFGADHGPLLD
                     APWPQIDESALAQDEIELALQINGKLRGTIRVPAKFSKAAIEAAALATPEFARFSEGA
                     PVKR"
BASE COUNT         1219 a         2166 c         2141 g         1097 t
ORIGIN      
        1 tcgcgcatcg tcgcaggcgc atcgttcgcg gcgatctggg ccgtggcttt cggtctgcgc
       61 ggtatcgagt ggaccaagcc gtatagccgg ccgatcagcg tcgcggtggt gcaaggcgcg
      121 attccgcagg acgagaaatg gctgaccgag aatctcgatg ccatcctcga gctatacaaa
      181 acccgcacgc gcgaggcgca tggcgcggat ctcatcgtct ggccggaatc ggccatcccg
      241 gacctggcta acaaccatat cgagttctac cgcgatgtct accaggaggc gagcgcgaaa
      301 ggttcgtcgc tgatggtcgg cacgctgcgc gcggaagaga acgcgaagac cggcgaagag
      361 gaatacttca actcggtgct gtcgatggac aagtcgacgc ccggtgtcgg ctggtacgac
      421 aagcatcatc tcgtgccgtt cacggagttc gtgcccgtgc ccggattcgt gcgtcagtgg
      481 ctaaggctca tgagcctgcc ttattcggat ttcaaccgtg gcgcggcgca gcaggcgccg
      541 ctggagtcag cggggcagcg tatctccgcc agcgtttgct acgaagacgc ctacggcgcc
      601 acccaattgc ccgcgctgcg caccgcgaca atgctcgtca acgtcaccaa cgacgcctgg
      661 ttcgggcatt ccccggcgcg ttaccagcat ctgcagatct cgcgcatgcg cagcctggaa
      721 gccgggcgtc ccatggtgcg ggcggccaac gatggcgttt cggcggtaat cgggcaccgg
      781 ggcgaaattg tcgccatggc gccggaatat gaagcgaacg taatgcgggc caccgtccaa
      841 ccgcgtatag gtttgacacc ctacgccggc acgggtaact gggcggtcgt gtgcctggcc
      901 cttgtgtttg ccctggccag cgcaatgctt aggcgtcgca ccaagacagt gtgaaggagt
      961 ggtccatggt ttcgcacccg ctgaacttcg ccaattccgc ccggtctact tcgacaacat
     1021 ttctctcctc acagaatcga gggtttgctg atatgtcgct attcgttgcg agacgtcttg
     1081 gcgcagtcgc gatcgtggcc ttcgcggtcg gcgcctgttc gccccagtct tccatcgccg
     1141 ctgcccaggc agaggtgccg cccagtaccg ttgccgccgc cgcgcaggcc cctacgtcgc
     1201 cgcaaccggt tggcacgctc aacgggcgcg tccttcccga cttcgccacg ctcgtcgagc
     1261 aggtgggtcc ggcggtcgtg aacgtgtccg tgctcgagaa gccgcatcgc gcaaacaagc
     1321 agtcttcaga agacgcggac gatcccgtgc aggaattctt ccgccgcttc ggcatgccga
     1381 acgggccgca gggccgcggc ttcgagcagc agccgcgcca gggcgaaggc tcgggcttca
     1441 tcgtcagtgc tgacggctac atcctcacca acgcgcacgt cgtcgcggat gcggatgaag
     1501 tcaccgtgcg cacgaccgat cgccgtgaat acaccgcgaa ggtggtgggt ctcgacaagc
     1561 gcacggacgt cgccgtactc aagatcgaag gcaaggatct cccggtagtg cgcctcggcg
     1621 acccgtccaa gctgcgcccg ggtgaatggg tgctcgccat cggctcgccg ttcacgttcg
     1681 agaacagcgt caccgcgggc atcgtcagcg cgaccggccg ttccatgccg ggtgaagatc
     1741 tggtgccgtt catccagacc gacgtcgcgg tgaacccggg caactcgggt ggcccgctgt
     1801 tcaacctcaa cggcgaggtg gtcggcatca actcgcagat ctacagccgc agcggcggct
     1861 acatgggtct gtcgttcgcc atcccgatcg acgtggccaa caacgtgcgc cagcagctcg
     1921 tgagcaccgg caaggtcact cgcggccgca tcggcgtgca gatccaggaa gtgaacgcgc
     1981 agttcgccga tgccttcggc ctcgaccgtc cgcgcggcgc gctggtcggg caggtcatcg
     2041 aaggcggtcc ggccgagaag gcgggcgtga agaccggcga cgtcatcctc agcgtcaacg
     2101 gcaagaacgt ggagcgatcc gcggcactcc cgggcgtcat ctccgcgatc aagccgggag
     2161 acaccgccaa ggtcgaagtg tggcgcgaag gcaattccaa gacgctcgcc gtcaaggtcg
     2221 aggaattcgc cgaagagacc cagaaggtgg cgaaccgcga cgtcgaggag ccggccaagg
     2281 ccgacaagct cggcctgtcc gttcgtccgc tcggcgccga tgagcgcaag agcgcttcca
     2341 ccgagggcta tctactggtc gaagacgtca cgggcccggc cgcgcaagcg ggcgtgcgtc
     2401 ctggcgacgt catcctcggc gtgaatggca aggcggtgaa gtccatcgcc gagctcaaga
     2461 acgccacggc cagcggttcg aagaccgtcg ccatcctgct cgagcgcgaa ggcaaccagc
     2521 tcttcctgcc catccgcatc agctgagaaa atggggtcag accccatttg ccagcaaaag
     2581 gggccagacc tgaaagggtc tggccttttc atttcagcgg caggtagata tccgtctccg
     2641 cgtcgccatc cggcacgtcg ggaaaaaatt tcacgcgctg catgaacagc gggaaatcgc
     2701 gcagctcttc gccgctcgtg ggcagccagt tcgcgtacag atgcatgatc gaccggccga
     2761 gctggtcgtc ggagccgcga tgccgcaaca gtgcgcatcg tccggcgggt atcacgaact
     2821 cggtcacacc ggactcgttc gtatcgatcg gccggtccgt cgccgcgcag agatcaaagc
     2881 ggaactcctc cggcggcgtc tcgtcggggt cgttgtggat gatgttgaac gtggcgctga
     2941 tctgcggtgg cagtttgtta gccttgcgcc attcgatgaa cctgcgaatg ctgtcgccca
     3001 ggcgaagcac gcttccacga tgctccaatc gcgccacgcg cgtcgatgga aaagtcgtca
     3061 cgcgcacgat gggcggtgat gtgatggtat tcatcgtggt ttgcctcaat tgaatgaacg
     3121 gccggaatcg cgaatgccac tcggtccagt ccggctggtc gcggaattcg ctcggcgact
     3181 gtccggagta ctgacggaac gcgcgcgcga aggcttccgg cccttcgtag ccggtcgcga
     3241 gagcgatgtc gttcaccgac aactcgggcc ggaatgcgag acgccatgac gcgcgacgca
     3301 tgcgcatgaa ctgcacgtag cgatacgtac tcattccgag gaatccggaa aactggcgat
     3361 ggaagtgatg ccgcgaaaac gcggcgatgg cactcagcgc atcgatcgac agcgcggttt
     3421 cggaattcgc ttcgatatgc gccagcacgc gcgcgaagcg ctcctggtaa cgctcgacag
     3481 tgcccggcag tttttcgcat tcgcccatgg ccgggactct aaccgtgccc gagccgcggc
     3541 cgcctgacgc ttgttgctgt tctgcgccgc tacagatcga agatcacgac gggcgccagc
     3601 gccacgagcg ccgctccggc ctgcaccgcc atcacgtaca tggcctccga gggaagccgc
     3661 ggcagccact tcaacgcgaa gaacgcgacg aacggcgcct ggcctgcgaa cagcagttga
     3721 aacacatgcg cggccgttcc ttcgtcggtc gcggccgcgt cgtgggtcgt cagcacgtga
     3781 atgagcacgg tggcgagcgc cacgcccgac atgacgagcg gaatgacggc gctggcgttg
     3841 cggaatagcg agcggctcac gcggcgcctt ccgccggaac cacccagaac gcggcttgca
     3901 cgagataagg cattgttcct ccgggtaacg cgccacacat tccctgatgg tgcgcaacag
     3961 gttcaacgcc tttttcgctt gtgcagcccg cctcgtgtat tctctccgcc cttctcaagc
     4021 ccctgaaaaa ccagacattc ccgatgcaag aacgatacga agcgcccgcg gtcgagcgtg
     4081 ctgcacaaga attttgggac cgcgaacgcg tattcgaagc gcgggaagac gctggcggcg
     4141 aggccggaaa gttttattgc ctgtccatgt tcccgtatcc gtcgggccgg ctgcacatgg
     4201 gccacgtgcg caactacacc atcggcgacg tgctcacgcg cttcgcgaaa atgcagggca
     4261 agagtgtcct gcagcccatg ggttgggatg cgttcggtct gccggccgag aacgcggcga
     4321 tcaagaacaa cgtgccgccc gccaagtgga cgcgcgacaa catcgcctac atgaaggcgc
     4381 agctgaagtc gctcggattc gccttcgact ggacgcgcga gatcgccacc tgcgatccgt
     4441 cttactaccg ctggaaccag tggctgttcc tgcgcatgct cgaaaagggc atcgcctatc
     4501 gcaagaccgg tgtcgtcaac tgggatccgg tcgaccagac cgtgctcgcc aacgagcagg
     4561 tcatcgacgg ccgcggctgg cgcacggggg ccatcgtcga gaagcgcgag atccccatgt
     4621 actacctcgg catcacgaag tatgccgacg agttgttagg cgacctcgag aagctgcccg
     4681 actggcccga gcgcgtgaag acgatgcagg ccaactggat cggcaagtcg gaaggttgcg
     4741 agatctcgtt cccgttcgcg ccggacaccc gggcgctcat ggctgccgat ggcgcgctca
     4801 aggtgttcac cacgcgcgcc gacaccttgt tcggcgtgtc gttcatggcg attgccgccg
     4861 aacatcccat cgcgctggcg gcgggcgcaa agaatcctgc attgcagacc ttcatcgacg
     4921 aatgccgccg cggcagcacg atggaagccg atatcgccac cgcggaaaag aagggcatgc
     4981 gcacggggct gcacgtgctg catccgttca ccggccagat ggtcgaggtc tgggtggcca
     5041 actacgtgct catggcctac ggcgaaggcg ccgtgatggg cgtgcccgcg cacgacgcgc
     5101 gcgacttcga attcgcgctc aagaacaagc tggccgtgat cacggtcatc cattcgaaga
     5161 ccggcgcata cgacaaggtg gtcgaaccct ggatcgacgc gtacaccgaa tacggcatca
     5221 cggtgaattc cggcgttttc gatggactcg acttccaggc ggccgtcgac gcgattgccg
     5281 tggcgctgga gaagaaaggg ctcggcagga aacgcacgca atggcgactg cgcgactggg
     5341 gcatctcccg ccagcgttac tggggttgcc ccgtgccgct gattcactgc gcggcctgcg
     5401 gcgtggtgcc ggtgcccgag aaggacctgc cggtcgtcct gccggaggac ctgatccccg
     5461 acggctcggg caatccgctc gccaagcacg gcgcgttcct gaacgtcgcg tgtccgacct
     5521 gcggccgcgc cgcgcggcgc gagaccgaca cgatggacac cttcgtcgac agcgcctggt
     5581 actacatgcg ctactgcgat ccgcatctcg aaagcgcgat ggtcggtgcc ggcaccaagt
     5641 actggatgcc gatggaccag tacatcggcg gcatcgagca cgcgatcctg cacctgctct
     5701 atgcgcgctt ctggaccaag gtcatgcgcg acatgaagct ggtcgccatc gacgagccgt
     5761 tcaccaagct gctcacgcag gggatggtgc tcaaccacgt ctgggcacgc acctcggcgc
     5821 agggcggcgt cgattacttc ccgcccgacg aggtcacgcc cgtcggcgag gctgaaggcc
     5881 ggccgagcag cggccggctg gccgacggca gcgcggtcga gtaccgcggc atgctcaaga
     5941 tgggcaagac cgagcgcaac ggcgtcgacc cgcaggatct catcgaccgc tacggcgccg
     6001 acacggcgcg cctgttcgtc atgttcgcgg cgccgcccga gcaatcgctc gactggaaca
     6061 acgccggcgt cgagggtgcg aaccgcttcc tcaagcgctt gtggaagttc gccgcgcggc
     6121 acgccgacac gatcgactgc gcggcggggc gccagcctgc gcgcggcggc gcgcccgcgc
     6181 tgcgccacga gatccactcg gtgctgcgcc aggtgagcta cgactacgag cggatgcaat
     6241 acaacaccgt cgtctcgggc gcgatgaagc tgctcaacgc cctcgagacg cacgccggcc
     6301 aagggtccgt cgcgccggcg ccgctgcgcg agggcttcgg catcctgctg cgcgtccttt
     6361 accccgtctg cccgcacacc acctggacgc tctggaacga gctcggcttc ggcgccgacc
     6421 acggcccgct gctcgacgcg ccctggccgc agatcgacga gagcgcgctg gcacaggacg
     6481 agatcgagct ggccctgcag atcaacggca agctgcgcgg cacgatccgc gtgccggcga
     6541 agttctccaa ggcggcgatc gaggcggcgg cgctcgccac cccggagttc gcccggttct
     6601 ccgagggcgc cccagtcaag cgg
//
LOCUS       DATDEU010000435         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1249291, whole genome shotgun sequence.
ACCESSION   DATDEU010000435 DATDEU010000000
VERSION     DATDEU010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249291"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="VIV63_10430"
     CDS             <1..1589
                     /locus_tag="VIV63_10430"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6125062.1"
                     /translation="QPFQDAMKHSRLSSTFLTAFACLLGVGATQAATAPVNITVRADT
                     PGAIIAPAIYGQFAEHLGRLIYEGIWVGEKSTIPNTRGYRNDVIAALKELGVPVLRWP
                     GGCFADEYNWREGIGPREQRPRKVNTHWGGVVEDNSFGTHEFMNFAELIGAETYVNAN
                     VGTGTPREMAEWLEYMTSDSTSSLAELRRKNGRDKPWTVNYFAMGNETWGCGGQMRPE
                     YYADLYRHYITFMKAPEGAKPVRIASGANGSGLPWTEVLMREAAKYMDALSVHYYTLP
                     TGKWDVKGAATRFTEDEWWATLVRTYRMEWIVTEHSKIMDKYDPEKRVGLYVDEWGTW
                     YDVEPGSNPGFLYQQNTLRDAVVAAVNLNIFHAHADRVRMTNIAQMINVLQAMILTDK
                     DKMVLTPTYHVFHMYKPFRGATHLPVTLEAPQRKLGETSAPTLTASAARGKDGRLHIA
                     IVNLDPREAIPVSLGIAGATPKKFSGTILTAAAMDAHNTFDKPAAVKPAAFDGARVSP
                     GAVRIDVPAKSIIVLSEAAQ"
     gene            1586..1897
                     /locus_tag="VIV63_10435"
     CDS             1586..1897
                     /locus_tag="VIV63_10435"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEY6125063.1"
                     /translation="MNYAFRMKLKPGVVEEYKRRHDEIWPELVQVLREAGIHDYSIFL
                     DEEDMHLFAVLKLRDNNKRDLLPGHPVMRRWWVYMQDLMYTQPDGRPNEWPLTPLFHL
                     D"
     gene            1931..>2345
                     /locus_tag="VIV63_10440"
     CDS             1931..>2345
                     /locus_tag="VIV63_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEY6125064.1"
                     /translation="MPNLAHGIQSADIDRTIQLLIRNLVEIKDTSGEFLLRLDDGRVI
                     DTKGWNDWEWTHGVGLYGIYKYYQMTRDARCREIMLHWFRDRFAAGTPTKNINTVAPF
                     LTLAYLYEESPDSSWLPYLDEWAEWLMDGLPRTEEN"
BASE COUNT          501 a          751 c          713 g          380 t
ORIGIN      
        1 cccaaccttt ccaggacgcc atgaaacaca gcagattgtc atcgacgttc ctgacggcct
       61 tcgcctgtct gctgggcgtg ggagccacgc aggccgccac ggcacccgtg aacatcacgg
      121 tacgcgccga cacgccgggc gccatcatcg cgccggctat ctacggccag ttcgccgaac
      181 acctggggcg gctcatctac gaaggcatct gggtcggcga gaagtcgacg attcccaaca
      241 cacgcggtta ccgaaacgac gtgatcgcgg cgttgaaaga actcggagtg ccggtgttgc
      301 gctggcctgg tggatgcttc gccgacgaat acaactggcg cgaaggcatt ggcccgcggg
      361 aacagcgtcc gcgaaaggtg aacacgcact ggggcggcgt ggtggaagac aactccttcg
      421 gcacgcacga gttcatgaat ttcgcggagc tgatcggcgc cgagacctat gtgaacgcca
      481 acgtgggcac gggcacgccg cgcgagatgg cggaatggct cgaatacatg acctcggaca
      541 gcacctcctc cctggcggaa ctgcgccgca agaacggccg cgacaaaccg tggacggtga
      601 actatttcgc gatgggcaac gagacctggg gctgcggtgg gcagatgcgg ccggagtact
      661 acgcggatct ctatcgccac tacatcacgt tcatgaaagc gccggaaggc gcgaagcccg
      721 tccggatcgc gagcggcgcg aatggcagcg gtcttccatg gaccgaggtg ttgatgcgcg
      781 aggcggcgaa gtacatggat gcgctcagcg tgcactacta cacgctgccg accggcaagt
      841 gggacgtgaa aggtgcggcc acccggttca ccgaagacga atggtgggca acgctcgtgc
      901 gcacctatcg tatggaatgg atcgtgaccg agcactcgaa gatcatggac aagtacgacc
      961 cggaaaaacg agtcgggttg tacgtggatg aatggggcac ctggtacgac gtcgagcctg
     1021 gctcgaatcc cggattcctc taccagcaga acaccctgcg tgacgcggtc gtcgcggctg
     1081 tgaacctcaa catcttccat gcgcatgcgg accgcgtacg catgaccaac atcgcgcaga
     1141 tgatcaacgt gctgcaggcc atgatcctga ccgacaagga caagatggtc ctgacgccca
     1201 cctaccacgt gttccacatg tacaagccgt ttcgcggcgc cacgcatctg cccgtgacgc
     1261 tcgaagcgcc acaacgcaag ctcggcgaga ccagcgcacc gacactcacc gcgtcggccg
     1321 cgcggggcaa ggatggccgg ctgcacatcg cgatcgtgaa cctcgatccg cgcgaagcca
     1381 tacccgtgag tctcggtatc gcgggcgcca ccccgaagaa gttctcgggc acgattctca
     1441 ccgccgccgc gatggacgct cacaacacct tcgacaaacc cgcagcggtg aagccggctg
     1501 cgttcgacgg cgcacgagtc tccccgggcg ccgtgcgcat cgacgtgccg gcaaagtcca
     1561 tcatcgtgct gagtgaggcc gcccaatgaa ttatgcattc cgcatgaagc tcaagcccgg
     1621 cgtcgtcgag gaatacaagc ggcgccacga cgagatatgg cccgaactcg tccaggtgct
     1681 gcgagaagcc ggcatccatg attactcgat cttcctcgac gaggaagaca tgcatctgtt
     1741 cgcggtgctg aagctgcgcg acaacaacaa acgcgatctg ctgcccggac atccggtcat
     1801 gcggcgctgg tgggtgtata tgcaggacct gatgtacaca caaccggatg gacggcccaa
     1861 cgaatggccg ctcacgccgc tgttccatct cgactaacga ccctctcgaa ccgtttccag
     1921 gagtttcttc gtgcccaatc tggctcacgg cattcaatct gccgacatcg accgaaccat
     1981 ccagctgctg atccgcaatc tcgtggagat caaggacacc tcgggcgagt tcctgctgcg
     2041 actcgacgac ggacgcgtca tcgacaccaa gggctggaac gactgggagt ggacgcacgg
     2101 cgtgggcctc tacggcatct acaagtacta ccagatgacg cgggacgcgc gctgccgcga
     2161 aatcatgctc cactggttcc gtgaccgctt cgccgccggc acgccgacca agaacatcaa
     2221 cacggtcgcg ccgttcctca ccctcgccta tctgtacgaa gagagccccg acagctcctg
     2281 gctgccgtac ctcgacgagt gggcggagtg gctcatggac ggcctgccgc gcaccgagga
     2341 aaacg
//
LOCUS       DATDEU010000436        10837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1832777, whole genome shotgun sequence.
ACCESSION   DATDEU010000436 DATDEU010000000
VERSION     DATDEU010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10837
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832777"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VIV63_10445"
     CDS             <1..1010
                     /locus_tag="VIV63_10445"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6125065.1"
                     /translation="LKLSQASDTSVTTSTGSHIIGSTPAMDRLRELILRVARSQAPVH
                     ISGESGTGKEMVARMIHASGPRTDGPFVPVNCGAIPSELMESEFFGHRRGSFTGAVSD
                     KDGLFQSAEGGTLFLDEVADLPLHMQVKLLRVIQEKTVRPIGEQRETRIDVRILSATH
                     KNLADLVAESRFREDLYYRINVIEIRVPPLRERRDDIRPLAEAILARLEKRVGIEGLK
                     LSGEAWRALETYEYPGNVRELENVLERAVTLSASGTIEAEHIRLRSAPRIATPQVTPV
                     ATTVRTEGIEALSDKLEHLERDAIIKALEQTRYNKTAAAKLLGVTYRALRYRIKKLEI
                     E"
     gene            complement(1067..2497)
                     /locus_tag="VIV63_10450"
     CDS             complement(1067..2497)
                     /locus_tag="VIV63_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125066.1"
                     /translation="MTAGQTALNCSLRDPDGQLVASGDRLFRVVDAESGFANLNLLER
                     PAFRGGMERGAIVRTWRPTDAEIPADVANRKQQAGALVLEHERLPFVSYPCEWSPLML
                     CDAALHTIDMLRLALSEGLILKDAAPTNIVFRGSRPVFVDFLSFTAREHGEFLWRARH
                     QFDACFLLPLLLNLEAGIPIAWTLRDFLHGVSHEQAQRILGLKSWLKPGLIGAVALPA
                     TLSRNVSAMGATHAARRKMDNDARALYSLEHQAKSLHSKLRGLRARLESGASRWAAYT
                     TSRAHYSPGELDQKRGFVRDALVRASPQTVLDLGANTGEFSELAAAASRVVAVDIDEQ
                     SVSGIAERARAGKLDIHPLVGNLAQPTPAEGWKNAETRSLLARLTGDCDLVLVLALMH
                     HLRVTGGIPFVEIADLLTALTRRHVVFELVPPSDPMFAAMARGRERLYGDCEVAPAEA
                     VLARSFNFLNRLALANGRMLLLLEKR"
     gene            complement(2508..2756)
                     /locus_tag="VIV63_10455"
     CDS             complement(2508..2756)
                     /locus_tag="VIV63_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125067.1"
                     /translation="MNSLRKLSGTTLRRGGIVAAVLVVTTADAHAYVDPGTGAMIVQI
                     IGALVAGALFYFRAIRDKIASWYSRLTSRSPAHKDQQG"
     gene            complement(2753..4306)
                     /locus_tag="VIV63_10460"
     CDS             complement(2753..4306)
                     /locus_tag="VIV63_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY6125068.1"
                     /translation="MAAIYPLWFIYFGNPGQASALYAAAATAVVLAVVAGLLWVAIRL
                     TRCRHVGAAALTVLVVAFFVYGPLHHLAVTISADPSWPFIVSDIFSLFASHVVLTLVM
                     LTVCAVLILLVVKRVAPGSARLTVACNFAVTALFVMLLARFAMSSWEERDLQMASAQA
                     GASDGRAMSVLGYNPDIYYIIVDGYARADVLSKYYDFDNSEYVAGLKSRGFKVNDSSQ
                     ANYNWTVLSLASSLNYAYLQDIAAPILADEEAIRGRNGYDKVARLVRDNRAANFLRTR
                     GYKFVHIRSSTPPTERNEYADEQVSCVGSLFDDEYLRTIVEVTWLQAAGAVATSDLAD
                     CHRQRLSSLQAQARQPGPKFVFAHFLPPHHPYLFDRNGNVLRHVTLSNQFDFQARLWE
                     DRAAYLDQLIYMNHSILAAIDKILAESARPPVIIVQSDHGPNLRTGLSREEMLSVRFA
                     NFAAYLLPGADNPMPGNCAPVNQFRYLFNHYFDAGLPILPNTSYFSEYSAILKMREIV
                     ADSPTGKTS"
     gene            4457..6571
                     /locus_tag="VIV63_10465"
     CDS             4457..6571
                     /locus_tag="VIV63_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY6125069.1"
                     /translation="MKNSAPRWHDGFLLTQVLALSLLVLSYLRTIGDSQSMAFRSHIL
                     DALYAGPIWGTKLGYNLLFFALSVVLVHVLYGLVCWGLGRLSARAWPSAKTTLRQHLL
                     LWFLLLTAGVLANNAATFSQSALGQPYAETMTALVLGTKLGHLIWIGVLSAGAVTVAL
                     AGFRWWSEGGRPSRPGYVAMGAASVAYIAVTAHSILPAKAPELSGKPNVILIGLDSLR
                     EDLVDEKFSPHATPHLAAFMKDGTWFSNAMTPLARTFPSMCAMFTGRRPHHSGAVMNL
                     LPRNLIDDSESLPRILARAGYKTVYATDEVRFANIDESFGFVQTITPPIGATEFMIEK
                     VADAPVTNLLVNTRLGRWLFPHVHANRGAAITYDPNAFVERIDRELSVNQPLFFNVHL
                     TLDHWPYTWAGSPIKKKDETARWPPYYLHAARRVDQQLGDIMTLLERRGLLRDAIVVV
                     YSDHGESFDAPHEALVPDDDPLIKELGLDPLWGHGTSVISAHQYRIVLGMRRYGADWQ
                     AGREFGVPVSFEDIAPTLVETLGLAHTARFDGRSLAPLLAGQKGAERGFAGRIRFTET
                     EYQLPAGFATQDGDLSPSKVAEAIAVYSVDRKTDRITVKKSHLGGLLLAREYAAIGEQ
                     NLVAAFPKKKEPGFFYLAIPLSGGAPRQLLSAPAPDEPELHALWNALHTEYGDAFQLT
                     RQPVATSTVANRERTIPQNVTK"
     gene            6758..7240
                     /locus_tag="VIV63_10470"
     CDS             6758..7240
                     /locus_tag="VIV63_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY6125070.1"
                     /translation="MIKTQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRSQVTEGL
                     NLAAPAKASVAEVFANTGAFPVNNTEAGVGAAADISGKYVSSVTITNGVITVVYGKQA
                     NANIVGDNVILTPGTSVNGDVAWVCGNHNTPSGISTPTAPALPSIEQKYLPQVCRTGA
                     "
     gene            7434..9185
                     /gene="pilB"
                     /locus_tag="VIV63_10475"
     CDS             7434..9185
                     /gene="pilB"
                     /locus_tag="VIV63_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEY6125071.1"
                     /translation="MNDNASLALGGTRSGLGGLPQRLVQDGVVEEAAMLTALQAAKDR
                     KSSFVTQLIAAGAAKARDIAVAASAEFGVPLYDLDSHSVELDTVKLVQDKLLQKHRCL
                     PLYRRGKRLFLGVADPTNLHAIDEIKFQTGMGIEAVVVEDDKLQKLIDKAAEQADAAM
                     PNLADDETGFDLENLDVSGGDDDAEGAGGISRDDVEDAPIVRFVNKLLLDAIRRGASD
                     IHFEPYEKMYRIRLRIDGVLKEVAQPPVQLAMKLSARLKVMSRLDIAERRVPQDGRIK
                     MKLSKTRAIDFRVSSCPTLFGEKIVCRILDPSSAMLGIDALGYEPFQREIYLKNLAKP
                     QGMILVTGPTGSGKTVSLYTGLNILNREDTNISTAEDPAEINLPGVNQVNVNNKVGLT
                     FASALRAFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHLVMSTLHTNDAPQTLTRLVD
                     MGVKPYAIATSVSLIIAQRLARKLCTQCKAIIDIPAEALLKEGFQEADVRAGMKIFAA
                     KGCSACSDGYKGRCGIYQVMPVTEALGRIIMEGGNAIDIADQAAKEGVWDLRRAGLEK
                     VKQGLTSIEEVNSVTIE"
     gene            9268..10437
                     /locus_tag="VIV63_10480"
     CDS             9268..10437
                     /locus_tag="VIV63_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY6125072.1"
                     /translation="MDKKGNRVSGKSLAPDEVALRADLRRQGIAVTRVKKQSQAFKSG
                     GKVKPEDIAVFSRQLATMLAAGIPLVQSFEIVGNGNDKPAMQKLILDIKADVEGGTSL
                     HEALARHPLYFDDLFVNLVEAGEQAGALETLLDKIATYKEKTEALKKKVKKALFYPTA
                     VLVVAVIVTIILLLFVIPQFESLFKGFGADLPAFTQMVINMSRWLQNNWYIFFGVIAG
                     AGYTFVYFKKRSQAMREFLDRALLKFPIIGPIMYKSAVARFARTLSTMFAAGVPLVEA
                     LESVAGATGNIVFENAVLKMRDEVATGQRLQRAMENTGLFPNMVIQMIAVGEEAGALD
                     AMSGKVATFYEAEVDNAVDSMSSLLEPLIMAILGVLVGGMVIAMYLPIFKLGAVV"
     gene            10531..>10837
                     /locus_tag="VIV63_10485"
     CDS             10531..>10837
                     /locus_tag="VIV63_10485"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY6125073.1"
                     /translation="MEFLEPLAALPTLFIGCCVVLGLLVGSFLNVVIYRVPVMMENAL
                     RAECASLANPDAPPPPTFNLMTPRSACPKCKAPITALQNIPVVSWLALGRKCANCKAP
                     "
BASE COUNT         2207 a         3421 c         3235 g         1974 t
ORIGIN      
        1 cactcaagct ttcgcaggcc agcgatacca gtgtcaccac gagcacgggc agtcacatca
       61 tcggcagcac gccggcgatg gaccggctac gcgagctcat cctgcgcgtc gcgcgcagcc
      121 aggcgcccgt gcacatttcc ggcgagtcgg gcacgggcaa ggaaatggtg gcgcgcatga
      181 tccacgcctc gggtccgcgc accgacgggc cgttcgttcc ggtcaactgc ggcgcgatcc
      241 cgtccgaact catggaaagc gaattcttcg gccaccggcg cggcagcttc accggcgcgg
      301 tttccgacaa ggatggtttg ttccagtcgg ctgaaggcgg aacgctgttt ctcgatgaag
      361 tcgcggacct gcccctgcac atgcaggtga aactgctgcg cgtcatccag gaaaaaaccg
      421 tgcgccccat cggcgaacag cgcgagacgc gcatcgacgt gcgcatcctc tcggccacgc
      481 acaagaacct tgcggacctc gtcgccgaat cccgatttcg cgaagacctc tactaccgca
      541 tcaacgtgat cgaaatccgc gtgccacctc tgcgcgagcg ccgcgacgat atccggccgc
      601 tggccgaagc gattctcgcg cggctcgaga aacgcgtggg catcgagggc ctcaagctga
      661 gcggcgaggc ctggcgcgcc ctcgagacct acgaataccc gggcaacgtg cgcgagctcg
      721 agaacgtgct ggagcgcgcc gtcacgttga gcgccagcgg caccatcgag gcggaacaca
      781 tccggctgcg ttcggcgccg cgcatcgcca ctccccaggt cacgccagtc gcgaccacgg
      841 tgcgtacgga gggaatcgaa gcgctcagcg acaagctcga acacctggag cgcgacgcca
      901 tcatcaaggc gctcgagcag acgcgataca acaagaccgc cgccgccaag ttgttaggcg
      961 taacctaccg cgcgctgcgc tatcgcatca agaagcttga aatcgaatga gcacggcgag
     1021 attggctcgt cgctccaatg gtgacttcgg cgtcgagaga agtgcctcag cgcttctcga
     1081 gcagaagcag catgcggcca ttggcgagcg ccagccgatt cagaaaattg aaggagcggg
     1141 ccagaaccgc ttccgcgggc gcgacttcac aatcgccgta gagtcgctcg cgtccgcgcg
     1201 ccatggctgc gaacatcggg tcgctgggcg gcactagctc gaatacaacg tgtcgccgcg
     1261 tgagcgcagt cagcaggtcc gcaatctcca caaacggaat gccgccggtc acgcgcaggt
     1321 gatgcatgag cgcgagcacg agtaccagat cacaatcacc cgtcaagcgc gcgagaaggc
     1381 tgcgtgtctc ggcgtttttc cagccctcgg ctggcgtggg ttgcgccaga ttgcccacca
     1441 ggggatgaat gtcgagcttg ccggcgcggg cacgctcggc aattccacta acggattgct
     1501 cgtcgatatc caccgccacg acccgcgagg cggcggcggc gagttcgctg aactcccccg
     1561 tatttgcgcc gagatccagc accgtctgcg gtgacgcccg cacaagcgcg tcacgcacga
     1621 agccgcgctt ctgatcgagc tcgcccggtg agtaatgcgc gcgactcgtg gtgtacgccg
     1681 cccatcgaga cgcccccgac tcgagtcgcg cacgcagtcc gcgcagcttg gaatgaagag
     1741 atttggcctg atgttcgagt gagtaaaggg cccgcgcgtc gttgtccatt tttcgacgcg
     1801 ccgcgtgagt agcccccatc gccgagacgt ttcgcgagag tgtcgcgggc agggccactg
     1861 cgccgatgag gccgggcttc agccacgatt tcaggccaag aatgcgctgc gcctgctcat
     1921 gcgatacgcc gtgaagaaaa tctcgcagag tccaggcaat cggaatgcca gcttcgagat
     1981 tgagcagcaa cggaagcagg aaacaagcat cgaactggtg acgagcccgc cacaggaact
     2041 cgccatgttc gcgcgccgta aaggacagga aatccacaaa caccggccgg ctgccgcgga
     2101 acacgatgtt agtcggcgct gcatccttga gaatgaggcc ttccgataac gccagccgca
     2161 acatgtcgat cgtgtgaagg gctgcgtcac acagcatgag cggcgaccac tcgcaggggt
     2221 acgacacaaa gggaagtcgc tcgtgctcga gcaccagcgc cccagcctgc tgtttgcgat
     2281 tcgcgacgtc cgcgggaatt tccgcatcgg tgggccgcca ggtgcggacg atggcgcctc
     2341 gctccatgcc gccgcgaaac gccggtcgct cgagtagatt gagattcgca aatccggact
     2401 cggcgtcaac gacacgaaac agcctgtcgc cactggcgac gagctgaccg tcgggatcac
     2461 gaagagagca gttgagcgcc gtttggcctg cggtcaccgg gcgggcctca tccctgctgg
     2521 tctttatggg ctggagaccg tgatgtcagc cgcgaatacc acgatgcgat cttgtccctg
     2581 attgcccgga agtaaaacaa cgcaccggca acgagggcac cgatgatctg cacgatcatc
     2641 gcgccggtgc ccggatccac gtaggcgtgt gcatcggcgg ttgtgacgac gagcacggca
     2701 gcgacgattc cgccgcgccg caatgtcgtt ccagaaagct ttcggagtga gttcatgaag
     2761 ttttccctgt tggcgagtcc gcaacgatct cgcgcatttt cagaatcgcg ctgtattcgg
     2821 agaagtagct cgtattcggc aggatcggaa gtccggcatc gaaatagtgg ttgaaaagat
     2881 atctgaactg gttgacgggc gcacagttgc ccggcatcgg gttatcggca cccggaagca
     2941 gatatgccgc gaagtttgca aatcgcacgg acaacatctc ctcgcgagac aaccccgtgc
     3001 gcagattcgg gccatggtcc gactgaacga tgatcaccgg cgggcgcgcg gactccgcaa
     3061 gaatcttgtc gatcgccgcc agaatggagt gattcatgta gatcaattgg tcgaggtatg
     3121 cagcgcggtc ctcccacaga cgcgcctgaa aatcgaattg attggagagg gtcacgtgcc
     3181 tcagcacgtt gccattgcga tcgaacaggt agggatggtg tggcggcaga aagtgcgcaa
     3241 acacgaattt cgggcccggc tgccgcgcct gggcctggag cgacgagagc cgttgccgat
     3301 gacaatccgc aaggtcggac gtggcgaccg caccggccgc ctgcaaccag gtcacttcca
     3361 cgattgtgcg caaatattca tcgtcgaaca ggctgcccac gcaggaaacc tgctcgtcgg
     3421 catattcatt ccgctccgtg ggcggcgtgg acgaccggat gtgtacgaac ttgtatccgc
     3481 gagtgcgcag gaaattcgcc gcgcgattgt cccgcacgag acgcgcgacc ttgtcgtatc
     3541 cgttccggcc cctgatagcc tcttcgtcag cgaggatggg cgccgcaata tcctggaggt
     3601 aggcgtagtt cagtgacgag gccagcgaca ggacagtcca gttgtagtta gcctggctcg
     3661 aatcgttgac cttgaatccc cggctcttca acccggcgac gtactccgaa ttgtcgaaat
     3721 cgtagtattt gctcaacaca tcggcacgtg cgtagccgtc cacgatgatg tagtagatgt
     3781 ccgggttgta gccgagcacg ctcattgcgc ggccgtcact cgcacccgcc tgtgccgaag
     3841 ccatctgcag atcgcgctcc tcccatgacg acatcgcgaa gcgcgccagc aacatgacga
     3901 aaagcgccgt caccgcaaag ttgcacgcga ccgtcaggcg ggccgaaccg ggcgctaccc
     3961 gcttgacgac cagtagaatc aaaaccgcac acacggtcag catcacgagc gtgaggacga
     4021 catgcgaagc gaacaggctg aagatgtcgc tcacgatgaa cggccacgaa ggatcggccg
     4081 agatcgtcac cgcgaggtga tgcaatggcc catagacgaa gaaggccacg acgagcactg
     4141 tcaatgctgc tgcaccgaca tgcctgcatc gcgtgagccg gatcgcaacc cacagcaaac
     4201 cggccaccac ggcgagcacg acggccgtcg cagcagcggc atacagcgca gacgcttgcc
     4261 cgggattgcc gaaatagatg aaccacaggg ggtaaatcgc ggccactaat ggataccacg
     4321 gccacccgac gacgcgttga accgtcggtg tgtttttcgc gaccgaattc attcggcgca
     4381 gcctacgaaa gcggcagata gcgctttgtg cgccaagccc ggttcccgca tttgacgagt
     4441 gatataagtc acatccatga agaattccgc gccgcgctgg catgacggct tcctgctgac
     4501 gcaggtcctg gccctgtcgc tcctcgtcct ctcctatcta cgcaccatcg gcgattcgca
     4561 gtccatggct ttcaggagcc acatcctgga cgcactctac gccggcccca tctggggcac
     4621 taagctgggc tacaacctgc tgtttttcgc gctcagcgtt gtcctggtcc acgtcttgta
     4681 cggccttgtg tgctggggcc tggggcgact ttcggcgcgg gcatggcctt ccgcgaaaac
     4741 cacactccgg caacacctgt tgctgtggtt cctgctgctg accgcaggag tactggccaa
     4801 caacgctgcc acgttctcgc agagcgcgct cggtcagccg tacgcggaga ccatgacggc
     4861 gctggttctc ggaacgaaat tgggccactt aatctggatc ggcgtgctga gtgcgggcgc
     4921 tgtcacggtc gcattggccg gatttcgctg gtggagcgaa ggcggccggc cctcgcgacc
     4981 agggtatgtg gcgatgggtg cggcgagcgt cgcctatatc gcagtcaccg cccactccat
     5041 ccttccggcg aaagcgccgg agctctcggg caaacccaat gtcatcctca tcggtctcga
     5101 ctcgttgcgc gaagatctcg tcgacgaaaa gttttcgccg cacgcgacac cgcatctcgc
     5161 cgctttcatg aaagacggca cgtggttctc caacgccatg acaccactgg cgcgtacctt
     5221 tccgtcgatg tgcgcgatgt ttaccggaag gcggccgcat cattcgggcg cggtgatgaa
     5281 cctgcttccg cgcaatctga tcgatgattc cgaatccctg ccacgaattc tcgcgcgcgc
     5341 tggctacaag acggtgtacg caaccgatga agtgcgtttc gcaaacatcg acgagtcgtt
     5401 cggttttgtt cagaccataa cgccgccgat cggcgccact gaattcatga tcgaaaaagt
     5461 cgccgacgct ccggtcacca acctgctggt gaacacgcga ctcggccggt ggttgttccc
     5521 gcatgtacac gccaatcgcg gcgccgcgat tacttatgac ccgaacgctt tcgtcgaacg
     5581 aatcgatcgt gagctcagcg tgaatcagcc attgttcttc aacgttcacc tgacgctgga
     5641 tcactggccg tacacctggg ccggctcacc tatcaagaaa aaggacgaaa cagcgcgttg
     5701 gcctccctat tacctgcacg cggcacggcg cgtggaccag caactcggcg acatcatgac
     5761 gctgctcgaa cgccgaggct tgctgaggga tgccatcgtc gtggtgtatt cggaccacgg
     5821 cgaatccttc gatgctccgc acgaagcgct ggtgccggac gatgatccgc tcatcaaaga
     5881 actgggcctc gatcctctct gggggcacgg cacgtcggtg atcagcgcgc atcagtaccg
     5941 gatagtgctt ggcatgcggc gctacggggc cgactggcag gcagggcgcg aatttggagt
     6001 tcctgtttct ttcgaagaca tcgcaccgac gctcgtggaa acactggggc tcgcccacac
     6061 ggctcgtttc gacggacggt cgctggcgcc gctgctcgcg gggcagaaag gagccgaacg
     6121 cggttttgca ggccgcatcc gcttcaccga aaccgagtat cagcttcccg caggcttcgc
     6181 gacgcaggat ggtgatctct cgccgagcaa ggttgccgag gcgattgcgg tctacagtgt
     6241 cgaccgaaaa acggatcgca tcaccgtcaa gaagtcgcac ctcggcggct tgctgctcgc
     6301 ccgtgaatac gcagcaatcg gcgagcaaaa cctcgtcgca gcgtttccga aaaagaaaga
     6361 gcccggattc ttttatctcg cgattcctct ctctggaggc gcaccgcgcc aactactttc
     6421 cgcaccggcg cctgatgagc cggagttgca tgcgctgtgg aatgcattgc acaccgagta
     6481 cggcgacgcg tttcaattga cccggcaacc tgtcgccact tcgactgtcg ccaatcgaga
     6541 gcgaactatt ccccagaacg tcacaaagtg acgaaatttg tcacttctgg cgaagaatga
     6601 ttatgggaaa cgcgtcttaa ctagtgcaat ttcagtgact tgggacacag cgcgagtggc
     6661 accgcctttg cattatcccc atcagcggac cggccaagcg ctggcgtcgc cggtcttacc
     6721 agtttctctt tgttttcatt tgttaggagc tcaacgcatg attaagactc aaaaaggttt
     6781 caccctcatc gaactgatga tcgtggtggc catcatcggc attctcgccg cgatcgcgat
     6841 cccggcttac caggattaca cgatccgctc gcaggtcact gaaggcttga acctggcggc
     6901 ccccgccaag gcttcggttg ccgaagtgtt tgcgaacacg ggcgctttcc cggtcaacaa
     6961 cacggaagcg ggcgtcggcg ccgccgccga tatcagcggc aagtacgttt ccagcgtgac
     7021 catcaccaac ggtgtcatca cggttgtgta cggcaagcag gcgaacgcga acatcgtcgg
     7081 cgacaacgtt atcctgaccc cgggaaccag cgtcaacggt gacgtggcct gggtttgcgg
     7141 taaccacaac acgccgtcgg gcatctccac gccgaccgcg ccggccctgc cctcgatcga
     7201 gcagaagtac ctcccgcagg tttgccgcac gggcgcctga tcggaagttg ctgagttgat
     7261 caagaagccc ctctccggag gggcttcttc tttttgggca ttttcttttt gggactgggg
     7321 tcacgcgagg cccgccctgc gtatgcgacc attagagcca catttaacaa ttgcctgact
     7381 gcggtcgatg agtagactag ccgcagcggg tctggtcgct cgcacgtaac cacatgaacg
     7441 acaacgcatc tctagcactc ggcggtactc gatccgggct cggtggcttg ccgcagcggc
     7501 tcgtgcagga tggcgtggtc gaagaagcgg ccatgctcac ggcacttcag gccgccaaag
     7561 accgtaaatc cagtttcgtc acccagctca tcgccgccgg cgccgccaag gcgcgcgaca
     7621 tcgcggtggc ggcctccgcc gaattcggcg ttccgctgta cgacctcgac tcgcacagcg
     7681 tcgagctcga caccgtcaag ctcgtgcagg acaagctcct gcagaaacat cgttgccttc
     7741 ctttgtatcg ccgcggcaag cggctgttcc tcggcgtcgc ggatcccacc aatctgcacg
     7801 ccatcgatga aatcaagttc cagaccggca tgggcatcga agcagtcgtc gtcgaagacg
     7861 acaagctgca gaagctcatc gacaaggccg ccgagcaggc cgacgcggcc atgccgaatc
     7921 tcgccgacga cgaaaccggt ttcgacctcg agaatctcga cgtcagcggt ggcgacgacg
     7981 atgccgaagg cgccggtggc atctcgcgag acgacgtcga agacgcgccc atcgtgcgtt
     8041 tcgtcaacaa gctgttgtta gacgccatcc gccgcggcgc gtcggacatc cacttcgagc
     8101 cctacgaaaa gatgtatcgc atccgtctgc gcatcgacgg cgtgctgaaa gaagtggccc
     8161 agccgccggt gcagctcgcc atgaagctgt cggcgcgcct caaggtcatg tcgcgtctcg
     8221 acatcgccga gcgccgcgtg ccgcaggacg gccgcatcaa gatgaagctt tcgaagactc
     8281 gcgccatcga cttccgcgtg agctcctgcc ccaccctgtt cggcgaaaag atcgtctgcc
     8341 gtattctcga tccgtcgtcg gccatgctcg gcatcgacgc gctcggctac gaaccgttcc
     8401 agcgcgagat ctacctcaag aacctcgcca agccgcaggg catgattctc gtcaccgggc
     8461 ccacgggctc gggcaagacc gtgtcgttgt acaccggcct caacatcctc aaccgcgaag
     8521 acaccaacat ctccacggcg gaagacccgg ctgaaatcaa cctgcccggc gtcaaccagg
     8581 tcaacgtcaa caacaaggtc ggcctcacct tcgcgagcgc gttgcgcgct ttcctccgcc
     8641 aggatccgga cgtcgtcatg gtcggcgaaa tccgcgacct cgagacggcg gaaatcgcca
     8701 tcaaggctgc ccagacgggt cacttggtca tgtccacgct gcataccaat gacgcgccgc
     8761 agacgctcac gcgcctcgtc gacatgggtg tgaagccgta cgccatcgcc acatcggtca
     8821 gtctcatcat cgcgcagcgg cttgctcgca aactgtgtac gcagtgcaag gccatcatcg
     8881 atatcccggc cgaggctctt ctgaaggaag ggtttcagga agcggacgtg cgcgcgggca
     8941 tgaagatatt cgcggcgaaa ggttgttcgg cttgcagcga cggctacaag ggtcgttgcg
     9001 gcatctacca ggtcatgccg gtcacggaag ccctgggccg aatcatcatg gaaggcggca
     9061 atgccatcga catcgccgac caggccgcga aagaaggcgt gtgggacctg cggcgcgcgg
     9121 gcctcgagaa ggtcaagcaa ggcttgacca gcatcgaaga agtcaacagc gtcacgatcg
     9181 aataaggact ctgaaatcat ggcaactgct gtagcgacaa gcggcgcaaa aggcgtcaag
     9241 cgcgattccc cattctcctg gtcgggaatg gacaagaaag gcaaccgcgt ctcaggcaag
     9301 agtctcgcgc ccgatgaagt cgcgttgcgc gccgatctgc gccgccaggg catcgccgtc
     9361 acgcgcgtca agaagcagag ccaggcgttc aagagcggcg gcaaggtcaa gcccgaagac
     9421 atcgcggtgt tcagccgcca gctcgccacc atgctggccg cgggtatccc gctggttcaa
     9481 tcgtttgaaa tcgtcggcaa cggcaacgac aagccggcga tgcagaagct cattctcgac
     9541 atcaaggccg acgtcgaagg cggcacctcg ctgcacgaag cgctcgccag acatcctctg
     9601 tatttcgacg atctgttcgt aaacctcgtg gaagctggcg agcaggccgg tgcgctcgaa
     9661 acgctgctcg acaagatcgc cacgtacaag gaaaagaccg aggcgttgaa gaagaaggtc
     9721 aagaaggcct tgttctaccc caccgcggtg ctcgtggtgg cggtgatcgt caccatcatc
     9781 ctgctgctgt tcgtcattcc gcagttcgaa agtctgttca agggcttcgg tgcagacctg
     9841 cccgcgttca cgcagatggt catcaacatg tcgcgctggt tgcagaacaa ctggtacatc
     9901 ttcttcggtg tcattgccgg cgcaggctat acgttcgtct acttcaagaa gcgttcgcag
     9961 gccatgcgcg agttcctcga ccgcgcgctg ctcaagttcc cgatcatcgg acccatcatg
    10021 tacaagtcgg ccgtcgcgcg tttcgcgcgc acgctgtcga ccatgttcgc ggccggcgtg
    10081 ccgctggtcg aagcgctcga atccgtcgcc ggagccaccg gcaacatcgt gttcgaaaat
    10141 gcggtgctga agatgcgcga cgaagtcgcc accggtcagc gcctgcagcg cgcgatggaa
    10201 aacacgggcc tgttccccaa catggtcatc cagatgatcg cggtcggtga agaagcaggc
    10261 gcgctcgacg ccatgtcggg caaggtcgcc acgttctacg aggccgaggt cgacaacgcg
    10321 gtcgacagca tgtcctcgct gctcgagcct ctcatcatgg ccatcctcgg cgtgctcgtc
    10381 ggcggcatgg tcatcgcgat gtacttgccc atcttcaagc tgggcgcggt ggtgtaaccc
    10441 acacctttcc acggcaatcc cgaaacgccg cacccatttc tagaagattg gggcggcgtt
    10501 tctttttgag cgaacgggtc ttatcgaatc atggagtttc tcgaaccgct cgccgccctt
    10561 cccacgctgt tcatcggctg ctgcgtcgtg ctgggactgc tggtcggcag tttcctcaac
    10621 gtggtcatct accgcgttcc ggtgatgatg gaaaacgcgt tgcgcgccga atgcgcgtcg
    10681 ctggcgaatc ccgatgcgcc gccgccaccc acgttcaatc tgatgacgcc gcgttcggcg
    10741 tgccccaagt gcaaggcgcc catcacggcg ctgcagaaca ttcccgtggt gagctggctc
    10801 gcgctcgggc gcaagtgcgc caactgcaag gcgccga
//
LOCUS       DATDEU010000437         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_958420, whole genome shotgun sequence.
ACCESSION   DATDEU010000437 DATDEU010000000
VERSION     DATDEU010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958420"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VIV63_10490"
     CDS             <1..906
                     /locus_tag="VIV63_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEY6125074.1"
                     /translation="PPIYLGIPMFASLSVTHGFLPPHPAPTAMLPMFGADIGTTLFYG
                     IMVAVPAMIIAGPVFARLFRNVKSNPLTLFVAPLRPESELPSAFNCFASALLPVALIA
                     CSMAVPLAPETWQPPLKFFTNQNIVMLLSLAIATYTLGLSRGMKMQALMDDYGNSVKD
                     IAVILLIIAGAGTLKEVFVATGVDKNISDALTGIAIDPLLLGWLIAGVIRVSLGSATV
                     AGMTAAGLMAPVVANAHVDPNLMVLAIGAGSLMFSHVNDSGFWMAKEYFNLTLKETLK
                     SWTLMEGIVGIVGIIGVLILEAAIS"
     gene            complement(869..>2578)
                     /locus_tag="VIV63_10495"
     CDS             complement(869..>2578)
                     /locus_tag="VIV63_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125075.1"
                     /translation="PARVYRRTDAWRPVNNEGLFASATAALNVTGVATSAVDSSAVYA
                     LDFAGRMLKSSTHGTAWNAPPPSLHTQLLRAVAVDPFFANVAYVATDEGAWRTIDGGF
                     SWQRSAGISVPIEKIVVSPSNRQVIYASSVWNTSLFRSNDGGLNFTETASLPATGKIG
                     ALAVDPANAQVVYAIVHNFLAKSTDGGGTWTMLPARDSTGADYNIGPAVLIDPVHPTT
                     LTIPSNAQHRGFIRSVDGGATWVRTTLNIGGNISYATAGVLHPTQPTLLVAAYEDQGV
                     GEYEVATDLAFAFTVQNAFAGSPFPAGHSAPMTILVMNAGPHAASPSTLRVTLPAWLS
                     GAATAGCSYAPPVLTCPLAPIQIGETRPITLTLAADSGSQTGTIQGSLTTHERDLNPA
                     SNLFAFDVAATFQSDLRIAASPDTSVLVGAAISRTLTVTNVGPSESVTNTLATTSSPN
                     LAVLTATSSRGTCSINAGSFGCQLGAMASGESATITFTGNASIAGEADIFATVLTSND
                     DRNPLDNVVRVRTTITSPPPPPPPPPPPKRGGGGGSLDWLAALFLGWLVARRSRLRVS
                     ARR"
BASE COUNT          428 a          836 c          858 g          456 t
ORIGIN      
        1 ccgcctatct acctcggcat cccaatgttc gcgtcactgt cggtgacgca tggcttcctg
       61 ccgccgcatc ccgccccgac ggccatgctg cccatgttcg gcgccgacat cggcaccacg
      121 ctgttctacg ggatcatggt cgcggtgccc gccatgatca tcgcgggccc ggtgttcgcg
      181 cggttgttcc gcaacgtgaa gtcaaacccg ctcacgttgt tcgtcgcgcc gctgcggccc
      241 gaaagcgagt tgccgagcgc gttcaactgc ttcgcgagcg cgctgctgcc ggtggcgctc
      301 atcgcctgct ccatggccgt gccgctggcg ccggaaacct ggcagccccc gctcaagttc
      361 ttcaccaacc agaacatcgt gatgctgctg tcgctggcca tcgccacgta cacgctcggc
      421 ctctcgcgcg gcatgaagat gcaggcgctg atggatgact acggcaattc ggtgaaggac
      481 atcgccgtga tcctgctcat catcgcgggc gcgggaacgc tgaaagaagt gttcgtcgcc
      541 acgggcgtcg acaagaacat ctccgatgcg ctgaccggca tcgccatcga cccgctgctg
      601 ctgggctggc tgatcgccgg cgtcatccgc gtgtcgctgg gctcagcgac ggttgccggc
      661 atgacggccg ccgggctcat ggcgcccgta gtggccaatg ctcacgtcga cccgaatctc
      721 atggtgctcg ccatcggcgc cggtagtttg atgttctcgc acgtcaacga ctccggcttc
      781 tggatggcca aggaatactt caacctgacg ttgaaggaaa ccctcaagag ctggacgctc
      841 atggaaggca tcgtcggtat cgtcggcatc atcggcgtgc tgatactcga agccgcgatc
      901 tcctagcaac cagccagccc agaaagagcg ccgccagcca gtcgaggctg ccgccaccgc
      961 caccgcgttt cggaggaggc ggcggtggag ggggcggcgg cgggctggtg atcgtcgtgc
     1021 gcaccctgac cacattgtcg agcgggttgc ggtcgtcatt gctggtcagc acggtcgcga
     1081 agatatcggc ttctcccgcg atgctcgcgt tgccggtgaa agtgatcgtg gcgctctcgc
     1141 cgctggccat cgcgcccaac tgacaaccga acgagccggc gttgatgctg caggtcccgc
     1201 gcgacgacgt ggcagtcagc accgcgaggt tcggcgacga ggtcgtggcc agcgtgttgg
     1261 tgaccgactc gctcggcccc acgttggtta cggtaagcgt gcgggagatg gccgcgccga
     1321 cgagaacact cgtgtccggc gacgcggcaa tccgaagatc cgactggaag gttgccgcca
     1381 cgtcgaatgc aaagaggttg ctggcgggat tgagatcgcg ctcgtgggtc gtcagggacc
     1441 cctggatggt gccggtctgc gagccggagt cggccgcgag cgtcagggtg atcgggcgtg
     1501 tctcgccgat ctggatgggt gccagcgggc aagtcagcac gggcggtgcg tacgaacaac
     1561 cggcggttgc ggcgcccgac agccatgccg gcagagtcac gcgcagcgtc gatggcgatg
     1621 cggcgtgtgg accggcattc atcaccagta tggtcatcgg cgcggaatgg ccggcgggaa
     1681 acgggctccc cgcaaatgca ttctgcaccg tgaacgcgaa ggcgagatcc gtggccacct
     1741 cgtattcacc caccccctgg tcctcataag cggcgacgag cagcgtaggt tgggtcggat
     1801 gcaggacgcc ggccgtcgcg taagaaatat tgccgcctat gttgagcgtc gtgcgcaccc
     1861 aggtcgcgcc gccatcgaca ctgcggatga agccgcgatg ttgcgcattc gacggaatcg
     1921 tcagcgtggt cggatgcaca gggtcgatga gcaccgcggg gccgatgttg tagtccgcgc
     1981 ccgtgctgtc ccgcgccggc agcatcgtcc acgtgccgcc gccatcggtc gatttcgcca
     2041 gaaagttgtg cacgatcgca tagacgacct gcgcgttcgc gggatcgacc gcgagcgcgc
     2101 cgatcttgcc ggtcgccggc aagcttgcgg tctcggtgaa attcaggccg ccgtcgttcg
     2161 accggaacag gctcgtgttc cacaccgagc ttgcatagat gacctgccgg ttgctcgggc
     2221 tcaccacgat cttctcgatg ggcaccgaaa ttccggcgct gcgctgccag gaaaatccgc
     2281 cgtcgatcgt gcgccacgcg ccttcgtcgg tcgcgacata cgccacgttc gcaaagaagg
     2341 ggtcgacggc caccgcacgc agcaactgcg tgtggagcga cggcggcgga gcattccatg
     2401 ccgtgccgtg ggtcgacgat ttcagcatcc gcccggcgaa gtcgagcgcg tacacggccg
     2461 aagaatccac cgccgatgta gccacgcccg tgacgttgag cgccgcggtc gcgctcgcga
     2521 agagtccttc gttgttgacc gggcgccacg cgtcggtgcg ccggtacacg cgtgcggg
//
LOCUS       DATDEU010000438         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1249737, whole genome shotgun sequence.
ACCESSION   DATDEU010000438 DATDEU010000000
VERSION     DATDEU010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249737"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /gene="purE"
                     /locus_tag="VIV63_10500"
     CDS             <1..401
                     /gene="purE"
                     /locus_tag="VIV63_10500"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY6125076.1"
                     /translation="YETKVVSAHRTPDLLFEYASTAQGRGIEVIIAGAGGAAHLPGMT
                     ASKTTLPVLGVPVQSKALSGLDSLLSIVQMPAGIPVATFAIGVAGATNAGLAAAAILA
                     NKRPEIDAALKKFRAEQTAKVLANPDPSKL"
     gene            412..1518
                     /locus_tag="VIV63_10505"
     CDS             412..1518
                     /locus_tag="VIV63_10505"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY6125077.1"
                     /translation="MTIGIVGAGQLGRMMALAGYPLGFDFLFLDKDAQTPGGQVGPLL
                     AGALTDRELLGQLSQRCEVVTFDWENIPVESLEGLPGNARISPPLRALGCAQDRLSEK
                     RTFELLGIPTTKYAAVDSRASLELAVKSIGLPGVLKTRRMGYDGKGQFVLRKPADLDR
                     AWEALGKSALLYENLVPFDAEVSVIAVRGVDGEVAFYPLNLNVHREGILRLTRAPYGN
                     ATLTRQAQRAARKVLEHFKYVGVLTIEFFVKRGKLVANEMAPRVHNSGHWTIEGADTS
                     QFENHVRAIAGLPLGSTRARGYSAMINLVGEMPTRDPLLGEPALHWHDYGKTPRPGRK
                     IGHLTLCETSAKTRDRRALKLLSRIDRATYLALR"
     gene            1577..2863
                     /locus_tag="VIV63_10510"
     CDS             1577..2863
                     /locus_tag="VIV63_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6125078.1"
                     /translation="MYIENFKLRELPFRLSPDPQFLYLSRAHARAKAYMESTIWFTDG
                     FVVVTGEIGSGKTTLIESFLRQLDSDVVIAQINQTQVNAVEFLQSVLVQFGFSPFKMK
                     KAELIATLNSFLIEQYAAGRKVLLIIDEAQNLSLKVLEEIRMLSGIEATKEKVLRIIL
                     AGQPELNEKLDSPELVQLAQRIRLRFHLGALSREDLRSYVRHRLDVAGADGREIFAED
                     TFPEIFRYAGGVPRLVNTLCDTAMMAAFNEDRDFVTPADIASAVNELQWAEFSSRANA
                     IAARASNGAHATGDRPSRVLSKLVLSTDGKAVAELHLMPGRKVVGRTPDNDLQIDSKF
                     ISRHHCQLVTGSDGITVIEDLNSTNGIMIRDKRVRRHSLRDGDVVTIGQHEILYVDEH
                     AGLLADTHDDLPRVDVDAANEDADDDSASEDAAGAR"
BASE COUNT          550 a          982 c          866 g          489 t
ORIGIN      
        1 cttacgagac gaaggtcgtc tcggcccacc gtactcccga cctgctgttc gaatacgcct
       61 ccacggccca gggacgcggc atcgaagtca tcattgccgg agccggtggc gcagcccatc
      121 taccgggcat gacggcgtcc aagaccacgt tgcccgtgct cggagtcccg gtgcagtcga
      181 aagcactgag tggcctcgac tcgctgctgt cgatcgtgca gatgccggcg ggcattcccg
      241 tggccacatt cgccatcggc gtggccggcg ccaccaacgc agggcttgcg gcggcggcga
      301 tcctcgccaa caaacgtccc gaaatcgacg cggcgctcaa gaaatttcgc gccgagcaga
      361 cggccaaagt gttggccaat cccgatcctt cgaagttgtg accctgcttc gatgaccatt
      421 ggcatcgtcg gcgccggaca gctcgggcgc atgatggccc tcgccggcta tccactcgga
      481 ttcgacttcc tgttcctcga caaggatgcg cagacgccgg gcggccaggt cggcccgctg
      541 ctggccggcg cccttacgga tcgtgagttg ttaggccagc tctcgcagcg ctgcgaggtg
      601 gtcacgttcg actgggagaa cattccggtg gaaagcctcg aaggcctgcc gggcaatgcg
      661 cgcatctcgc cgccgctgcg ggcattgggg tgcgcgcagg atcgcctcag cgaaaaacgc
      721 actttcgaac tcctcggaat tcccaccacg aaatacgccg cggtcgattc gcgcgccagc
      781 ctcgagctcg cggtcaaatc catcgggctg ccgggcgtcc tcaagacgcg gcgcatgggt
      841 tacgacggca aaggccagtt cgtgttgcgc aaacccgcgg acctcgaccg cgcctgggaa
      901 gcactcggca aatctgcgct gctctacgaa aatctcgtgc cgttcgacgc cgaagtgtcg
      961 gtgatcgcgg tgcggggcgt ggatggcgaa gtcgccttct atccactcaa cctcaacgtg
     1021 caccgcgagg gcatcctgcg gctcacgcgt gcaccctacg gaaacgcgac gttgacccgc
     1081 caggcgcagc gcgccgcgcg caaggtcctg gaacatttca agtacgtcgg cgtgctgacc
     1141 atcgagttct tcgtcaagcg cggcaaactc gtggccaacg aaatggcgcc gcgcgtgcac
     1201 aactccggcc actggaccat cgaaggcgcc gacaccagcc agttcgagaa ccacgtgcgc
     1261 gccattgccg gactgccgct gggctcgacc cgtgcgcgcg gatactcggc catgatcaat
     1321 cttgtaggtg aaatgccgac acgcgatccg ctgctgggag agcctgccct gcattggcac
     1381 gactacggaa agacgccccg gccgggccgc aagatagggc atttgacgtt atgcgaaacc
     1441 tccgccaaaa cccgggaccg gcgggctcta aagctcctgt ccaggatcga ccgcgcgact
     1501 tacctcgcac ttcggtaacc ccgcataggg gcttccctgc ccggacgcca gctttaccgt
     1561 aatatcggcc gctgcgatgt acatcgagaa cttcaaactc cgtgagctgc cgttccgctt
     1621 aagtccggat ccgcagttcc tgtacctgtc ccgcgcccac gcgcgtgcca aggcgtacat
     1681 ggaatcgacc atctggttta cggatggctt cgtggtggtc accggcgaaa tcggctcggg
     1741 caagacgacg ctcatcgaga gcttcctgcg ccagctcgac tccgacgtcg tcatcgcgca
     1801 gatcaaccag acccaggtca acgcggtcga attcctgcag tcggtgctgg tccagttcgg
     1861 cttctcgccc ttcaagatga agaaggccga actcatcgcc acgctcaaca gcttcctcat
     1921 cgagcagtac gcggccggcc gcaaggtgct gctgatcatc gacgaagcgc aaaacctgtc
     1981 gctcaaggtg ctcgaagaaa tccgcatgtt gtcgggcatc gaagccacga aagagaaggt
     2041 gctgcgcatc attctcgccg gccagcccga gctcaatgag aagctcgact cgccggaact
     2101 cgtgcagctg gcgcagcgca tccgcctgcg cttccacctg ggcgcgctgt cgcgcgaaga
     2161 cctgcgctcc tacgtgcgcc accgtctcga cgtggccggc gcggatggcc gcgagatatt
     2221 cgccgaagac actttcccgg agatcttccg gtacgccggc ggcgtgccgc gtctcgtcaa
     2281 cacgctgtgc gacaccgcga tgatggccgc gttcaacgaa gaccgtgatt tcgtcacgcc
     2341 cgcggacatc gcctcggccg tcaacgaact gcagtgggcg gagttttcat ctcgcgccaa
     2401 cgccatcgcc gcgagagctt ccaatggcgc gcatgccacc ggcgatcgcc catcgcgagt
     2461 gctctccaag ctcgtgctgt ccacggacgg caaagccgtg gccgagctgc atctcatgcc
     2521 cggccgcaag gtcgtcggac gcacgcctga caacgatctg cagatcgaca gcaaattcat
     2581 cagccggcac cactgccaac tcgtgaccgg cagcgatggc atcaccgtca tcgaagatct
     2641 gaacagcacc aacggcatca tgattcgcga caagcgcgtc cgccgccatt cgttgcgcga
     2701 cggcgacgtg gtgaccatcg gccagcacga gatcctctac gtggacgaac acgcggggct
     2761 gctcgccgac acccacgacg atctcccgcg tgtggacgtc gatgccgcca acgaagacgc
     2821 ggacgatgac tccgcttccg aggacgcggc tggcgcgcgc tgaaactgcg gcccgataga
     2881 tcgacgc
//
LOCUS       DATDEU010000439         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1687374, whole genome shotgun sequence.
ACCESSION   DATDEU010000439 DATDEU010000000
VERSION     DATDEU010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687374"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="VIV63_10515"
     CDS             complement(<1..1951)
                     /locus_tag="VIV63_10515"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEY6125079.1"
                     /translation="MLTRIRSLLVANRGEIAIRVMRAANELGIRTIAIYSQEDRFSLH
                     RTKADEAYLVGRGKAPVDAYLDIEDILRIAVEAKADAIHPGYGFLSENPEFVEACNRE
                     GVIFVGPTPEIMRTLGNKVAARNLATSASVPVMPATPPLPADIEESKTQAAKVGYPVM
                     LKASWGGGGRGMRVIESDAQLTELLPVARREAKAGFGNDEVYLEKLVRKARHIEVQIL
                     GDTHGNLVHLFERDCTVQRRNQKVVERAPAAFLTDAQRNELCTYALQIGRAVKYRNAG
                     TVEFLQDAETGKFYFIEVNPRIQVEHTVTEAVTNIDLVKAQIRIADGAKIGDEASGVP
                     KQEDIHLTSHALQCRVTTEDPENGFSPDYGKITAYRSPAGFGIRLDAGTAYAGAILTR
                     SYDSMLVKVTAWAPNPAEAIARMHRALWEFRIRGVSTNLRFLDQVITHPKFVNADYTT
                     KFIDETPELFKWEKKRDRATRILSFIGEVIVNGNPEVKGRLKPKGMVLARLPKVNVAK
                     APPPGTKQKLDELGAAGFAKWMLAQKQVLITDTTMRDAHQSLLATRMRQPDMAAIAPY
                     YAHMLPQLFSVECWGGATFDVAMRFLKEDPWARLEQLRTLMPNLLLQMLLRSSNAVGY
                     TNYPDNVVRAFVKQAAVSGIDVFRVF"
BASE COUNT          341 a          635 c          663 g          398 t
ORIGIN      
        1 cgaacacgcg gaacacgtcg atgccggaaa ccgccgcctg cttcacgaac gcgcgcacga
       61 cgttgtcggg gtagttggtg taccccacgg cgttggaaga gcgcagcagc atctgcaaca
      121 gcagattggg catgagcgta cgcaattgct cgaggcgcgc ccagggatct tccttgagga
      181 accgcatcgc gacgtcgaac gtggcgccgc cccagcactc gaccgagaac agctgcggca
      241 acatgtgcgc gtagtaaggc gcaatggctg ccatgtcggg ctgacgcatg cgggttgcca
      301 gcagcgactg atgcgcgtcg cgcatggtcg tgtcggtgat cagcacctgc ttctgcgcca
      361 gcatccactt cgcgaaaccc gcggcgccga gctcgtcgag cttctgcttc gtgcccggcg
      421 gcggcgcctt tgcgacgttc accttgggca gccgcgcaag caccatgccc ttcggcttca
      481 ggcgcccctt cacttccggg ttgccgttga cgatgacttc gccgatgaag ctcaggatgc
      541 gggtggcgcg gtcgcgcttc ttttcccact tgaagagctc gggcgtctcg tcgatgaact
      601 tggtggtgta gtcggcgttc acgaacttcg ggtgcgtgat cacctggtcg aggaaacgca
      661 gattcgtcga cacgccgcga atgcggaact cccacaacgc gcgatgcatg cgcgcgatgg
      721 cctcggccgg gttgggcgcc cacgccgtga ccttcacgag catggaatcg tacgaccgcg
      781 tgaggatggc gccggcgtac gccgtgcctg catcgagacg gatgccgaag cccgcgggcg
      841 agcggtacgc ggtgatcttg ccgtagtcgg gcgagaagcc gttttcggga tcttccgtgg
      901 tcacgcggca ctgcagtgca tgcgaggtga gatgaatgtc ttcctgcttc ggaacgccgc
      961 tcgcctcgtc gccaatcttc gcaccgtcgg cgatgcggat ctgcgccttc acgaggtcga
     1021 tgttggtgac ggcctcggtc accgtgtgtt ccacctggat gcgcggattc acttcgatga
     1081 agtagaactt gccggtctcg gcgtcctgca ggaattcgac ggtgccggcg ttgcggtact
     1141 tcaccgcgcg gccgatctgc agcgcgtagg tgcaaagctc gttgcgttgc gcgtcggtca
     1201 gaaatgccgc cggcgcgcgc tcgaccacct tctggttgcg acgctgcacg gtgcagtcgc
     1261 gttcgaacag atgcacgagg ttgccgtgag tgtcgcccag gatctgcact tcgatgtggc
     1321 gcgccttgcg caccagtttc tcgaggtaca cctcgtcgtt accgaatccg gccttggctt
     1381 cacgacgcgc gacgggcagg agttccgtga gctgcgcatc gctttcgatg acgcgcatgc
     1441 cacggccacc gccaccccaa ctcgccttga gcatgaccgg gtagccgacc ttggccgcct
     1501 gcgtcttgga ctcttcgatg tcggccggca gcggcggcgt ggccggcatc accggcacgc
     1561 tcgcggaagt agctaggttg cgcgccgcca ccttgttgcc gagcgtgcgc atgatctccg
     1621 gcgtggggcc gacgaaaatc acgccctcgc gattgcaggc ctcgacgaac tcggggttct
     1681 cggagagaaa cccgtatccg gggtggatgg catcggcctt ggcttcgacc gcgatgcgca
     1741 ggatgtcttc gatgtcgaga tacgcatcca ccggcgcctt gccgcgaccc accaggtagg
     1801 cctcgtcggc cttggtccga tgcaacgaaa agcgatcctc ctgcgaatag atcgcgatgg
     1861 tgcggatacc cagttcgttg gcagcgcgca tgacgcgaat cgcgatttcg ccgcgattgg
     1921 cgaccagcag gctgcggatg cgagtcagca tggatggctt cttctaatat gattccccgg
     1981 gacgggaatc ttaacgaaaa cggcgttctg aaccggcgtc aatttcggtg ctgataa
//
LOCUS       DATDEU010000440        10452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1979473, whole genome shotgun sequence.
ACCESSION   DATDEU010000440 DATDEU010000000
VERSION     DATDEU010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979473"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1198
                     /locus_tag="VIV63_10520"
     CDS             179..1198
                     /locus_tag="VIV63_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY6125080.1"
                     /translation="MTKIGPQLTKQSPLHVAIACYPGAQATCIHGLTDLFMYADYFAR
                     EHAAKQQAVVRVTHWRHGADGDALECTYDSDPGPFANPAVCIVPACQMAPPKVALAPQ
                     SVAWVKQRHSEGAVVAAVCGGVFLLAESGLLAGRRATTHWMFAQELQRRFPDLDVDSD
                     RLVIDESDVITAGGVLAWADMGLSLVERLLGPTVMCTTARFMLMDPPGREQRAYGEFT
                     PHLQHGDKTILSIQHWLQANSTTACSVSRLADRVNLSERTFLRRFMKATGVKPSEYHQ
                     RLRVARSRELLEFTRDSVEQIAAATGYEDPGGFRRIFKRVVGLSPTEYRRRFQRAPQV
                     TQGVR"
     gene            complement(1183..1983)
                     /locus_tag="VIV63_10525"
     CDS             complement(1183..1983)
                     /locus_tag="VIV63_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6125081.1"
                     /translation="MFVLGAALLAYIVLNVGSWWALRQLPAVPEVVVVPAGGDAVEGE
                     RLASILGCKGCHGADLGGAEDCFERPGQYRLTCPNITAARDLYTDRDLVILLRHGRKI
                     DGALVDFMPWDMYAQLTDSDLANVIAYVRAAPYVDKPALHDSQYSLAVRWQMLRGEYP
                     WQNDLADYDTKPLDGPAERGRYLASVSCPECHAPNLRGYEGDTAPSLTVVQAYPPEAF
                     ARLMREGITMTGRESASGLMTAVARDRFSHFTAEEVVALKAYFDQRTP"
     gene            complement(2051..4186)
                     /locus_tag="VIV63_10530"
     CDS             complement(2051..4186)
                     /locus_tag="VIV63_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125082.1"
                     /translation="MAVASLIPVAILSFALLITNAHEQRDRLLRVSEDTMLALMSAAD
                     AELKSTIAALDALAASPRLVRGDFAGVREEALELLGRRSSWLNVVVLDADRQYMNARV
                     PEDEPLPDVTSREMVEQTIRTQAPLVGQIIDAPVLKTSAVAVLVPVVRKDKVQYVIAA
                     VVRPDYFLQLLDLENVASEGVIAILDPHYNVVARSLNQSGSVGKRASTTLVRMLEGGA
                     MRGSATTRTLEGVPVYTVFRRSEFSGWVSAVGIPMRSVDAPVIRSYIVFGSAVVLSIF
                     LGLVAATLVGRTIVGPMHELEESAARVGRGEAPSMPSTHLLEVRRVAVALAKAHDERA
                     ASFSREHDARLAAERASKAKDEFLAMLGHELRNPLAAITNASQVIERQRLTLDPGIAT
                     ATAIITRQSRHLARMTDDLLDAGRVILGKISLARAPLDLSAAVAGTLDGLRNTGRVGD
                     HVFEVSLNPVWIFADATRIDQIVGNLLTNAIKYTPAGGRISVSTRREGAQALFEVTDT
                     GIGLEPELLPRAFELFVQGERGLDRSQGGLGIGLTLVQRLAELHGGSAHAQSSGSGKG
                     ATFTVRLPAIEAPAADLPPDIAGHARRQCQIAIVEDNDDARVSLRMLLELEGHAVHEA
                     AEGTAGVPLITANPAITVAFIDIGLPGMSGYDIARAVRARRGDSIRLVAMSGYGGEQD
                     VAKGLEAGFDAYIVKPADISALRPEIAKA"
     gene            4482..6743
                     /locus_tag="VIV63_10535"
     CDS             4482..6743
                     /locus_tag="VIV63_10535"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HEY6125083.1"
                     /translation="MRAFSTWSAIAFAAIASSLFTACDDDKKAVVPSITAPASPLGNG
                     VVGTAITSVDFVSSGTAPITYSVSAGLLPPGLILDASSGRLSGSPTLTGIFEFTITAT
                     NRAGSDDARYLQSILQRPSITTPASPLTTAVGGMPAAALTFEASGSMPISWAVTAGAL
                     PPGMALNAATGAYSGTPTAAGDYSFTVMATNAAGTDSDAFTQQVTAPAANAHALINGN
                     SIAAVAITFPSGLEAPLALTGLAAGDALVSIDRRPQNGFLYGLGYNATAGSVQLYTIS
                     TTTGLAAPIGATGTFIAADGVTPVPVGAGAATTFGMDFNPTVDRVRVVNSAGQNFRLN
                     PNNGAFVDGTAGGALNMDGGINGPTTGVQETAYTNNAPNSTVTTQYALDLTTDALCIQ
                     NPPNAGTQTLCQALSSPVDAVLGFDIAPGINVATASTPATGSGIAVLKLSGQTNETLA
                     TVNLATGAVAALGTIGPGGITGLAVHQPAAVRFVGLSADGTQLLRFSSNAMSTAVTVA
                     ITGIAAGETLAGIDYRPQTGQLYTLGVSDVANAATLYLLDPQTGAATIVGTAGGVTFV
                     DGGGTPVDLPAATAGYGFDFNPTVDRIRVTTQTGLNFRINPNNGAPVGTITDGAINGG
                     ATGASGAAYTNSFGQSLTGGVTTQYVIDAATHQLFIQNPPNSGTVTAGVAITIGGVPL
                     DFTEVSGFDIPSEVRTATSGAPVTSGIAYAALTVAGEQRMYSIDLVTGVATDIGAPAA
                     NLSGLATGQTALR"
     gene            complement(6817..7713)
                     /locus_tag="VIV63_10540"
     CDS             complement(6817..7713)
                     /locus_tag="VIV63_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125084.1"
                     /translation="MTFELSALHKHMPIIDGFPRPQWAAIDSLISQQPEAESHTQWVA
                     AARAWMEATARHLGSPYAVAETDHFFLLSPLSARQTGLLRVFIEKAWKQIGVKLEGLG
                     ETGVLGKCVVMLFDSHDRYYEYSAHFYGEGEHPISTGVFLNAEYGHTAIPFFDIAETE
                     ATLAHELTHCFLRSLPIPLWLNEGLAVTMEDEMCANRPLRMDSQRLAEHADYWTAETI
                     QDFWSGQSFQRVDEGIDLSYELARYCVRALAHDVPMFIEFAKSASFEDGGEAAATKVY
                     GGSLGGVIHQFFGPGDWSPRVR"
     gene            complement(7754..8536)
                     /locus_tag="VIV63_10545"
     CDS             complement(7754..8536)
                     /locus_tag="VIV63_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125085.1"
                     /translation="MSVTSPQVAQRLRRIQNALGVTADGMLGPETLTALEKHLELGVT
                     RKAVSLSCSCKSLELIVAFEIGSRAQYERLYAQPTWPGGESGVTIGIGYDLGFTSKQQ
                     IEADWDPFLEKPDRAALAAVQGAKGAVAAQLARGLRHIRVPIEAAEAVFYASTLPRFA
                     RIVRDAFPGVEKLPLDAQGAMLSLVFNRGASLAGDRRREMRAIRAELAGGRASLDVVA
                     DQFEAMQRLWPDVRGLRERRVREAALVRGARRRYAAGERIDV"
     gene            8670..9359
                     /locus_tag="VIV63_10550"
     CDS             8670..9359
                     /locus_tag="VIV63_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY6125086.1"
                     /translation="MTILNRRSLLLSLGAAVAPVALASTRKHVWKFDNIRKVDGHAVR
                     VEGMPKVIDSPVGRAVLFDGQKDALFIGNHPLAGAKTFTAELVFRPDGGAFEQRLLHL
                     ESDETPAVEPGKSSTRMLFEIRVVKDSWYLDAFMVGAGYRQAMMATDKLHPLGRWYHV
                     AKTYDGQFFRSYVNGVLQMEVAMPFTPQGPGRATVGARMNQVTHFQGAIRQARFTHEA
                     LPVKTFSRRIP"
     gene            complement(9340..>10452)
                     /locus_tag="VIV63_10555"
     CDS             complement(9340..>10452)
                     /locus_tag="VIV63_10555"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6125087.1"
                     /translation="LAPMSTETGWSRAGISFAMTINFLVMAFGSFGWGAASDRWGARI
                     VTLCGAALLGLALVLASNAKSLMSFQLTFGVLVGLSASAFFAPMIATVTGWFETRRNL
                     AVSLVSAGMGMAPLTISPLASWLITHDGWRAAMFDIGILAWVLLIPAAMFVRRPPVAE
                     DSPAQTKATGGEREISVGTALTSPQFLVLGFTFFLCCAAHSGPIFHVVSYAMYCGLGS
                     MAAVSVYSVEGLAGLGGRLLLGVLADRYGAKRILVTALAVQAMAIAAYLAVNELDEFY
                     ALAVIFGVAYGGGMPLYASLARDYFGQRIMGTVFGAATMLSSLGMSIGPVAGGWVFDT
                     FNNYAWLYISSAAVAVGAVLLALLFPKPRLITVSAG"
BASE COUNT         1911 a         3492 c         3282 g         1767 t
ORIGIN      
        1 catcgcacgt ggcgcgcata tccgcattca ctccgcatgc ttcgtgcaat acgacgatcg
       61 tcgggcgtgc cagcgcatcc ggctgcactg tgtgcgcatc gaaatcgccc tctgctgatg
      121 caattttcat gggttgcgcg aagatagcgg cgcaccgccg tgccggggat ggactgaagt
      181 gacaaagatc gggccgcaac tgacaaaaca atcgcccttg cacgtggcca tcgcctgtta
      241 tcccggcgcg caggccacgt gcattcacgg gctcaccgac ctcttcatgt acgcggacta
      301 cttcgcgcgc gagcatgccg cgaagcagca ggccgtggtg cgcgtgacgc attggcggca
      361 cggcgccgac ggcgatgcgc tcgaatgcac atacgacagc gacccgggtc cgttcgccaa
      421 tcccgccgtc tgcatcgtgc cggcatgcca gatggcgccg ccgaaagtgg cgctcgcgcc
      481 ccaaagtgtg gcgtgggtaa agcagcggca ctccgaaggt gccgtggtcg ccgcggtatg
      541 tggcggagta ttcctgctcg cggaatccgg cttgttagcc gggcgacgcg cgacgactca
      601 ctggatgttc gcgcaggaat tgcagcgccg gtttcccgac ctcgacgtgg attcggaccg
      661 gctcgtcatc gatgaatcgg atgtgatcac cgcgggcgga gtgctcgcct gggccgacat
      721 ggggctgagc ctggtggaac gcctgctggg cccgaccgtc atgtgcacca ccgcgcgatt
      781 catgctcatg gatccgccgg ggcgcgaaca acgtgcttac ggcgaattca cgccccatct
      841 gcagcacggc gacaagacca ttctgtcgat ccagcactgg ctgcaggcga attccacgac
      901 ggcctgcagc gtgagccgcc tggcggatcg cgtcaacttg agcgaacgca ctttcctgcg
      961 tcgcttcatg aaggccacgg gcgtcaaacc cagcgaatat caccagcggc tgcgcgtcgc
     1021 gcgaagccgc gagttgctcg aattcacgcg cgactccgtg gagcagattg cggcggcgac
     1081 gggttatgag gacccgggcg gattccgccg catattcaaa cgcgtggtgg gcctgtcgcc
     1141 gacggaatac cgccgccggt ttcagcgcgc gccgcaggtg actcagggcg tccgctgatc
     1201 gaagtaggct ttcagtgcga ccacttcttc ggccgtgaaa tgcgaaaagc ggtctcgcgc
     1261 aaccgcggtc atcagtccgc tcgcgctttc gcgaccggtc atcgtgatcc cctcgcgcat
     1321 caggcgcgca aacgcctcgg gaggataggc ctgcacgacg gtgagactcg gcgcggtgtc
     1381 gccttcgtat ccacgcaggt tcggcgcatg acactcgggg cacgacacgc tggcgaggta
     1441 gcgcccgcgc tccgccggcc catcgagcgg cttcgtgtcg tagtccgcca gatcgttctg
     1501 ccagggatat tcgccgcgca acatctgcca gcgcacggcc agcgagtatt gggagtcatg
     1561 caaggcgggc ttgtccacgt aaggtgccgc acgcacgtag gcaatgacgt tcgcgaggtc
     1621 gctgtccgtg agctgcgcat acatgtccca gggcatgaaa tcgacgagcg cgccatcgat
     1681 cttgcggccg tggcgcagca ggatcacgag atcgcggtcg gtgtagagat ctcgcgccgc
     1741 cgtgatgtta gggcaggtca ggcgatactg gccgggcctc tcgaagcagt cttcagcacc
     1801 acccaggtcg gcgccgtgac atcccttgca accgagaatg gaggcgagac gttcgccctc
     1861 cactgcatct ccgccggcgg gaaccaccac cacttcggga accgccggca actgccgcag
     1921 cgcccaccag cttccgacat tcagcacgat ataggcgagc agcgccgcgc cgagaacaaa
     1981 caagattgag ttgacgattg tttgcctgcg catgtcgtga gtgtgccacg gttttcgcgc
     2041 aggcacggga tcaggccttc gcgatctccg ggcgcagtgc cgagatgtcg gctggcttta
     2101 cgatgtacgc atcgaagccg gcctccaggc ctttcgcgac atcctgttcg ccaccgtaac
     2161 ccgacatcgc caccagccgg atcgagtcgc cgcggcgcgc gcggacggct cgcgcaatgt
     2221 catagccgct catgcccggg agaccgatgt cgatgaaagc cacggtgatc gcgggattgg
     2281 cggtgatcaa cggcacgccg gcggttcctt ccgccgcttc atgcaccgca tgtccctcga
     2341 gttcgagcaa catgcgcagg ctgacgcgcg catcgtcatt gtcttccacg atggcgatct
     2401 ggcactgccg gcgcgcatgg cccgcgatgt cgggcggcaa atcggcggca ggcgcttcga
     2461 tggccggcaa tcgcacggta aacgtggcgc ccttcccgct cccggagctt tgcgcgtgcg
     2521 cggatccccc gtgcaattcc gcgagtcgct gcaccagcgt gagaccgatg ccgagcccgc
     2581 cctgcgaacg gtcgaggccg cgttcgcctt gcacgaacag ctcgaatgca cgcggcaaca
     2641 actcgggctc gagcccgatg ccggtgtcgg tgacctcgaa caatgcctgg gcaccttcgc
     2701 gccgcgtgct cacgctgatg cggccgccgg cgggcgtgta cttgatcgcg ttggtcaaaa
     2761 ggttgccgac gatctgatcg atgcgcgtcg catccgcgaa tatccagacc gggttgagcg
     2821 acacttcgaa cacgtgatcg cccacgcgcc ctgtgttgcg cagtccatcg agcgttccgg
     2881 ccactgcggc ggaaagatcg agcggcgccc gcgccagtga aatcttgccg aggatgaccc
     2941 gccctgcatc gaggaggtcg tcggtcatgc gcgccaggtg ccgcgattgc cgcgtgatga
     3001 tcgcggtggc cgttgcgatg ccggggtcga gcgtcaggcg ctgccgttcg atgacctgcg
     3061 aggcattggt gatcgccgcc agcggattgc gcaattcgtg gcctaacatc gccaggaatt
     3121 cgtccttcgc cttgctggcg cgttccgcgg ccaaccgcgc gtcgtgctcg cgcgaaaagg
     3181 atgcggcccg ctcgtcgtgc gctttcgcca gcgcaaccgc gacgcgccgg acttcgagca
     3241 ggtgcgtgga cggcatggaa ggcgcctcgc cgcgtcccac gcgggccgca ctctcttcga
     3301 gctcgtgcat cgggcccacg atcgtgcggc ccacgagcgt ggcggcgacc agacccagga
     3361 agatcgagag caccacggcg ctgccgaaca ctatgtatga gcgaatcacc ggcgcatcga
     3421 cggaccgcat tggaatgccg acggcgctca cccagccgga gaattccgag cgccggaaca
     3481 cggtgtacac cggcacgcct tcgagcgtgc gcgtggtcgc gctgccgcgc atcgcgccgc
     3541 cctccagcat cctgaccagg gtggtcgacg cgcgtttgcc gacagagccg gattgattga
     3601 gcgaacgcgc caccacgttg taatgggggt cgaggatggc gatgacgcct tcggaggcga
     3661 cgttctccag gtcgagcagc tggagaaaat aatccggcct caccacggcc gcgatgacgt
     3721 actgcacctt gtccttgcgt acgaccggca ccagcaccgc gaccgccgaa gtcttgagta
     3781 ccggtgcgtc gattatctgg ccgaccagtg gcgcctgggt gcgaatggtt tgttcgacca
     3841 tttcgcgcga ggtcacgtcc ggcagcggct cgtcttcggg cacacgcgcg ttcatgtatt
     3901 gccggtcggc gtcgagcacc acgacgttga gccagctcga ccggcgcccc agcagttcca
     3961 gcgcttcttc gcgcacgccg gcgaaatcgc cgcgcaccag ccgcggactg gccgcaagcg
     4021 catcgagcgc ggcgatggtg ctcttgagtt ccgcatcggc ggcgctcatg agcgccagca
     4081 tcgtatcttc gctgacgcgc aacagccggt cgcgttgctc gtgcgcgttg gtgatcagca
     4141 atgcgaacga caggatggcg accggaatca ggcttgcgac cgccacgaga aaaacgcgcc
     4201 gctccagttt gccgagcccc aggcgccata agcctttcga gctgagaatt gttttttgtt
     4261 ccgtggtttc aggcacgccc aagcatcata ggttgccgca gcggcgacca ctatcgcctg
     4321 tactcctaca cgcccgcgta aattcgccgg gattcgctta tacgaaacta ctaggcgtcc
     4381 ggatgcgcgg tgaaaaaaga ttgccacgcg tgccaggcgc cgcgcatcca cgcgacatat
     4441 acccccgtag cgcccctgaa cgacacactc aggggactct catgcgcgca ttcagcacct
     4501 ggtccgccat cgccttcgcg gcgatcgcgt cttctctttt caccgcttgt gacgatgaca
     4561 agaaggccgt ggtgccttcc atcaccgcac ccgcctcacc actcggtaac ggtgtagttg
     4621 gtacggcgat cacctccgtc gatttcgttt cgagcggcac ggcgccgatc acgtacagcg
     4681 tttccgccgg cctgttgccg cccggactca tcctcgatgc gtcttcggga agattgtccg
     4741 gctcgccgac gcttacggga atattcgagt tcaccatcac cgcgacgaat cgcgccggca
     4801 gcgatgacgc gcgctacctg caatccattc ttcagcggcc ctcgatcacc acgcccgcgt
     4861 cgccgctcac cactgcggtg ggtggaatgc ccgcggcggc gctgacattc gaagcgagcg
     4921 gctcgatgcc catctcctgg gccgttaccg cgggagcatt gccgccgggc atggcgttga
     4981 atgcggccac gggagcgtac tcgggaaccc ccaccgccgc gggcgactac tcgttcacgg
     5041 tgatggcgac caacgcggcc ggaaccgaca gcgatgcgtt cacgcagcag gtcacggctc
     5101 cggccgcgaa tgcgcacgcg ctgatcaatg gcaacagcat cgccgcggtc gcaatcacct
     5161 tcccttcggg actcgaagcg ccgctggcgc tgacgggact cgccgcgggt gatgcgctcg
     5221 tcagcatcga tcgccgaccg cagaacggtt ttctgtacgg cctcggttac aacgccaccg
     5281 cgggctccgt gcagctctat acgatttcga cgactacggg acttgcagcg ccgattggcg
     5341 ccaccggcac gttcatcgcc gccgatggcg tgacgccggt gcccgtgggc gcgggtgcgg
     5401 ccacgacgtt cggcatggac ttcaacccca cggtcgatcg cgtgcgcgtg gtcaacagcg
     5461 ccggccagaa cttccgcctg aaccctaaca acggcgcatt cgtcgacggc accgccggcg
     5521 gcgcgctgaa catggatggc ggcatcaacg gtcccacgac cggcgtccag gaaacggcat
     5581 acaccaacaa cgcacccaac agcacggtga cgacgcagta cgcgctcgac ctcaccaccg
     5641 acgcgctctg catccagaat ccgccgaatg ccgggacgca gacgctttgt caggcgctca
     5701 gctcgcccgt ggatgcggtg ctcggcttcg acatcgcacc gggcatcaac gtggcgacgg
     5761 cgagcacacc tgcaaccggc tccgggattg cggtgctcaa gctttcaggt cagacgaacg
     5821 agacgctggc caccgtgaat ctcgctaccg gtgctgtcgc ggcgctcggc accatcggcc
     5881 cgggtggaat caccgggctc gcggtgcatc agcccgcggc ggtgagattc gtcggactct
     5941 ccgccgacgg cacgcagctg ctgcgctttt cgagcaacgc gatgtcgaca gcggtcaccg
     6001 tcgccatcac gggcatcgcc gccggcgaga cactggccgg catcgactac cgcccgcaga
     6061 ccggtcagct ctacacgctc ggcgtgtccg acgtggcgaa cgctgccacg ttgtacctgc
     6121 tcgatccgca gacgggcgcc gcaacgatcg tgggcaccgc gggcggcgtg accttcgtcg
     6181 atggcggcgg aacgcccgtc gatctgcccg ccgcaacggc cggctatggc ttcgacttca
     6241 atccgaccgt cgatcgaatc cgcgtcacca cgcagaccgg gctcaacttc cgcatcaacc
     6301 ctaacaacgg cgcgccggtg ggtacgatca cggacggggc catcaacggc ggcgccaccg
     6361 gtgcatccgg agcggcctac acgaacagct tcggtcagtc actcacgggc ggcgtcacca
     6421 cgcagtacgt gatcgacgct gcgacccatc agctgttcat ccagaacccg cctaacagcg
     6481 gcaccgtcac cgccggtgtc gcgatcacga ttggtggcgt gccgctggat ttcacggagg
     6541 tcagtggctt cgacattccg tccgaggtgc gcacggccac ttcgggtgcg ccggtgacat
     6601 ccggcatcgc gtatgccgcg ctgaccgttg ccggcgagca gcgcatgtat tcgatcgatc
     6661 tggtgacggg cgtggccacg gacattggcg cgccggcagc gaacctgtcg ggattggcga
     6721 cgggccagac cgcactgcgt tgaacgatcc tgggtgacat cgcgtcgagg gcggtggcgc
     6781 ttctgaaggc caccgcccat caccgttctt cgcaggtcag cgcacgcggg gcgaccaatc
     6841 tccgggcccg aagaactggt gaatcacgcc gcccaaacta ccgccgtaaa ctttcgtcgc
     6901 ggcggcttcc ccgccatctt cgaacgaagc agatttcgcg aactcgatga acatcggaac
     6961 gtcatgagcc aacgctcgca cgcaataacg tgccagttcg tagctgagat cgatgccttc
     7021 atcaacgcgc tgaaacgact gcccgctcca gaagtcctgg atggtctccg cggtccaata
     7081 gtcggcgtgc tcggccagcc gctgggagtc catgcgcaag ggacgattcg cgcacatctc
     7141 gtcttccatg gtgacggcca ggccttcgtt gagccacaac ggaatcggca ggctcctgag
     7201 gaagcagtgc gtgagctcgt gcgccagcgt cgcctccgtt tccgcgatgt cgaagaacgg
     7261 aatggcggta tggccatatt cggcgttcag gaagacaccg gtgctgatcg gatgctcgcc
     7321 ctcgccgtag aaatgcgcgc tgtactcgta gtacctgtcg tgcgaatcga agagcatcac
     7381 cacgcatttg ccgaggactc cggtttcacc gaggccctcg agtttcacgc ctatctgttt
     7441 ccacgccttt tcgatgaaga ctctcaatag tcccgtttgc cgggcactca gcgggctcag
     7501 caggaagaag tggtcggtct ccgccacggc gtacggagat cccaggtgac gcgcggttgc
     7561 ttccatccag gctcgcgccg ccgcgaccca ctgcgtgtga ctctcggctt ccggctgctg
     7621 cgagatcagg gaatcgatgg ccgcccattg tggccggggg aatccatcga tgatcggcat
     7681 gtgtttgtgc agcgcgctca actcaaacgt catggtgcgt gaatatagcc gcggcggcgg
     7741 caagtcacga gagctaaacg tcgatgcgct cgccggcggc ataacgccgg cgggcgccgc
     7801 gcacgagcgc cgcttcgcgc acgcgccgct cgcgcaggcc gcgaacatcc ggccacaacc
     7861 tctgcatggc ctcgaactga tccgcgacga cgtcgagtga cgcgcgcccg ccggccaatt
     7921 ccgcgcgaat ggcacgcatc tcgcggcgcc gatcacccgc gaggctcgcg ccgcggttga
     7981 acacgagcga caacattgca ccctgcgcgt cgagcggcag tttttccacg cctgggaatg
     8041 catctcgcac gatgcgtgcg aaccgcggca gcgtgctggc gtagaacacg gcctctgccg
     8101 cttcgatcgg aacccggatg tgccgcagcc ctcgcgcgag ttgtgcggcg accgcaccct
     8161 tggcgccctg aaccgccgcg agcgccgcac ggtcgggttt ttcgaggaat gggtcccaat
     8221 ccgcttcgat ctgctgtttc gacgtaaaac ccaagtcgta gccgatgcca atggtgacgc
     8281 cgctctcccc gcccggccac gtcggctgcg cgtaaagtcg ttcgtactgc gcacgcgagc
     8341 cgatttcaaa agccacgatg agttcgagcg acttgcacga acatgagaga ctcaccgcct
     8401 tccgcgtcac ccccagttcg aggtgtttct cgagcgcggt caacgtttcg ggaccgagca
     8461 tgccgtcggc cgtgacgccg agtgcgttct ggatccggcg cagccgctgc gccacctgtg
     8521 gactggtgac tgacatcgtc gatgaagtta ggacgcgcca tgaaaagtgt caacgcgtcg
     8581 cgctaactag tcgcctgcaa tcgtgacttt gtaccgctcc gcgggtgccg cactgcaacc
     8641 tgtcaatcac cgctggtaat ctttcgacca tgaccatcct gaaccgccga agtctcctcc
     8701 tctccctggg cgcggccgtc gcaccggttg ccctggcgtc cacgcgcaaa cacgtgtgga
     8761 aattcgacaa catccgcaag gtcgacggac acgcggtgcg ggtggaaggc atgccgaagg
     8821 tcatcgacag ccccgtcggt cgcgcggtgt tgttcgacgg gcaaaaagac gcgctgttca
     8881 tcggcaacca tccgctggcg ggcgcgaaaa ccttcacggc cgaactggta ttccggccgg
     8941 acggcggcgc attcgagcag cgcctgctgc acctggaatc agacgagacg ccggccgtcg
     9001 agcccggcaa aagcagcacg cgaatgctgt tcgagatccg agtcgtgaaa gatagctggt
     9061 acctggatgc gttcatggtc ggcgccgggt atcggcaggc gatgatggcg acggacaaac
     9121 tccatccgct cggccgctgg tatcacgtgg cgaaaacgta cgacggacag ttcttccgtt
     9181 cgtatgtgaa cggcgtgctg cagatggagg tggccatgcc tttcacgccg caagggcccg
     9241 gacgcgccac ggtgggcgca cgcatgaacc aggtcactca cttccagggc gctatccggc
     9301 aggcgcggtt cacgcatgaa gcattgccgg tgaagacttt cagccggcgg ataccgtaat
     9361 cagcctcggt ttgggaaaca gcagtgcaag caggaccgcg cccacggcga cggccgcgga
     9421 tgaaatgtag agccacgcat agttgttgaa cgtatcgaac acccagccac cggcgaccgg
     9481 tccaatggac atgccgagac tcgacaacat cgtggccgcg ccaaacacgg tgcccatgat
     9541 gcgctggccg aagtagtcgc gcgccagcga ggcatacaac ggcattccgc cgccgtacgc
     9601 gacgccgaat atcaccgcca gcgcgtaaaa ctcatccagt tcgttcaccg cgagataggc
     9661 ggcgattgcc atcgcctgca ccgccagcgc cgtcaccagg attcgcttcg cgccgtagcg
     9721 atccgcgagc acgcctaaca gcaggcgtcc gccgagcccc gcgagaccct cgacgctgta
     9781 gacgctcacg gccgccatcg aacccagtcc gcagtacatc gcataactga cgacgtggaa
     9841 gatagggccc gaatgcgcgg cgcaacacag aaagaaggta aagcccagta cgagaaattg
     9901 cggtgaggtg agcgccgtac ccacggagat ctcacgttcg ccgcctgtgg ctttcgtctg
     9961 cgccggtgaa tcttcggcaa ccggcgggcg gcgcacgaac atcgcggccg gaatgagcag
    10021 cacccacgcg aggatgccga tgtcgaacat cgccgcacgc cagccgtcgt gcgtgatcaa
    10081 ccagctcgcc aacggcgaga tggtcagcgg cgccatgccc atgccagccg aaacgagcga
    10141 cacggcgaga ttgcggcggg tctcgaacca gcccgtgacg gtggcgatca tcggcgcgaa
    10201 gaacgcgctc gcggagagcc cgaccagaac gccaaacgtg agctggaaac tcatgagcga
    10261 cttcgcgttg ctcgcaagca ccagcgcgag cccgagcagc gcggcgccgc acagtgtgac
    10321 gatgcgcgca ccccagcgat cgctggcagc tccccagcca aacgagccga acgccatgac
    10381 gaggaagttg atggtcatcg cgaaagaaat tcccgcgcgc gaccagccgg tctccgtcga
    10441 catcggtgcc ag
//
LOCUS       DATDEU010000441         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1250186, whole genome shotgun sequence.
ACCESSION   DATDEU010000441 DATDEU010000000
VERSION     DATDEU010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250186"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VIV63_10560"
     CDS             <1..485
                     /locus_tag="VIV63_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6125088.1"
                     /translation="AIADARAAGGKVISGEKILEGKGNFVTPTIVLAENDWPIVQHET
                     FAPVLYLIPFDSIDEAIEMQNASAHGLSSAIFTDRLQHAEAFLSASGSDCGIANVNIG
                     TSGAEIGGAFGGEKDTGGGRESGSDSWKAYMRRQTNTINWSRELPLAQGITFDIKEGP
                     "
     gene            complement(482..1858)
                     /locus_tag="VIV63_10565"
     CDS             complement(482..1858)
                     /locus_tag="VIV63_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125089.1"
                     /translation="MSRPSLSRRLLLSVFIVLLVFFGLTVFLLDRVFQRAAERSLREL
                     IDAQMVALIAAADPDGPESVTPTAVLETRFDTPGSGLYAEIRSASGESIWRSQSTVGT
                     AVQFGPPVEGGQRTFFYTDIAGTSIRLAVASRGIVWDDLHGQPARFTFSVATSLEVYE
                     AQLASFRQQMIGWFVGLAVLLVATLALLLRWLLKPVRRLEREIKEVEGGTREHLGEAW
                     PRELSAVTSNLNALLDGERTRIRRYRDTLGNLAHSLKTPLAVMRQALGAGGASKAALD
                     AEIDRMSGIIEHQMKRAAASGGVLLGQAPVDVGPVVAELRSALLKVYGNKDMSLEAAI
                     GPSVQFIGDRADLTELLGNLLDNACKWCRSRVRVEASIEKDSESRQALRITLDDDGAG
                     IAEADRARVLQRGGRADEATPGHGIGLSMVHDTVELYGGSMRIDSSSLGGARFELRLP
                     GRTVPGGV"
     gene            complement(1878..2552)
                     /locus_tag="VIV63_10570"
     CDS             complement(1878..2552)
                     /locus_tag="VIV63_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6125090.1"
                     /translation="MRALVVEDEASLREGLREQLVKQGFTVDVAADGEEGLFAGSEYP
                     LDIAVVDLGLPKMSGLDLIRALRGAGKKFPILILTARDRWQDKVEGLQAGADDYVAKP
                     YHFEEVLARMQALLRRAGGWASPILECGPIRLDTRSQTVTLNTTPIELTTFEYRILEH
                     MMLRAGDVISKTELTERLYDQDFERDINVIEVFIGRLRRKLDPDETLHPIETLRGRGY
                     RFALAR"
BASE COUNT          494 a          893 c          880 g          465 t
ORIGIN      
        1 aagccattgc cgacgcccgc gccgcgggtg gcaaggtgat cagcggggag aagatcctcg
       61 agggcaaggg caacttcgta acaccgacta ttgttcttgc cgagaacgat tggccgatcg
      121 tgcaacacga gaccttcgca cccgtgctct atctcattcc cttcgacagc atcgacgagg
      181 cgatcgagat gcagaacgca tcggcgcacg ggctgtcgtc ggcgatcttc accgaccggc
      241 tgcaacacgc ggaggcgttc ctgtccgcgt cgggcagcga ctgcggcatc gccaacgtga
      301 acatcgggac atccggcgcc gagatcggcg gcgcgtttgg tggcgagaag gacacgggag
      361 gcggacgcga gtccggttcg gattcgtgga aggcctatat gcgccgccag accaacacca
      421 tcaactggag ccgcgagctg ccgttggccc agggcatcac gttcgacatc aaggaaggcc
      481 cttaaacgcc gcctggcacc gttctacccg gaagtcgaag ctcgaagcgc gcaccgccca
      541 ggctcgagct gtcgatgcgc atggatccgc cgtacaactc gacggtgtcg tgaaccatcg
      601 acagaccgat gccatgtccg ggggttgctt catcggcgcg gccaccacgc tgcaagacgc
      661 gcgcacgatc ggcttccgcg atacccgcgc catcgtcatc gagggtgatt cgcaacgcct
      721 ggcgcgattc gctgtccttc tcgatcgatg cctcgacgcg cactcgcgaa cggcaccact
      781 tgcaggcgtt gtccagcagg ttgccgagca actcggtgag atcggcgcga tcgccgatga
      841 attgaacgct tggcccgatc gccgcttcga gcgacatgtc cttgttgccg tagaccttga
      901 gcagcgcgct gcgcagctcg gcgaccacgg ggcccacgtc cacgggcgcc tggcctaaca
      961 acacaccgcc gctggccgcg gcgcgtttca tctggtgttc gatgatgccg ctcatgcgat
     1021 cgatctcggc gtcgagcgca gccttgctcg cgccaccggc gccgagcgcc tggcgcatga
     1081 cggcgagcgg cgtcttgaga ctgtgcgcga gattgcccag cgtgtcgcga taacgacgga
     1141 tgcgcgtgcg ttcgccatcg agcagcgcat tgagattgct ggtcaccgcc gacagctcac
     1201 gcggccatgc ctcgccgaga tgttcgcgtg tgccgccctc cacctctttg atctcccgct
     1261 cgagccgccg caccggtttc agcagccagc gcaacaacag cgccagcgtg gcgaccagca
     1321 ggaccgcaag acccacgaac catccgatca tctgctggcg aaaactcgcg agctgcgctt
     1381 cgtagacttc gagactggtg gcgacgctga acgtgaagcg cgccggctga ccatgcaggt
     1441 cgtcccagac gatgccgcgg ctggccaccg ccagccggat cgaagtgcct gcgatatcgg
     1501 tatagaagaa cgtgcgctgg ccgccttcga ccggcggtcc gaactgaacc gcggtgccga
     1561 ccgtcgactg cgagcgccag atgctctcgc cgctcgccga gcggatctcc gcatacaaac
     1621 cagaacccgg tgtgtcgaaa cgcgtttcca gcaccgcggt gggcgtgacc gattcaggac
     1681 cgtcgggatc ggccgcggcg atgagcgcca ccatctgcgc atcgatgagt tcgcgcaggc
     1741 ttcgctccgc ggcgcgctga aaaacccggt cgagcaggaa tacggtgagc ccgaagaaca
     1801 ccaggagcac gatgaacacc gacagcagga gccggcggct tagggaagga cgcgacaagc
     1861 gcgagcttcc tcagtcccta ccgggcaagg gcgaagcggt agccgcgtcc gcgcagggtt
     1921 tcgatgggat gcagtgtttc gtcgggatcg agtttgcgcc gcagacggcc gatgaagacc
     1981 tcgatgacgt tgatgtcgcg ctcgaagtcc tggtcgtaca ggcgttcggt gagctcggtc
     2041 ttggagatga cgtcgccggc gcgcagcatc atgtgttcga ggatccggta ttcgaacgtc
     2101 gtgagttcga tcggcgtggt gttgagcgtg acggtctgcg agcgcgtatc caggcggatc
     2161 gggccgcatt cgagaatggg gctggcccag ccgccggccc ggcgcaacag cgcctgcatg
     2221 cgcgccagca cttcttcgaa gtgatacggc ttggcgacgt aatcgtccgc gcccgcctgc
     2281 aatccttcga ccttgtcctg ccagcgatcg cgcgccgtca ggatgaggat gggaaatttc
     2341 ttgcccgcgc cgcgcaacgc gcggatgaga tcgagcccgg acatcttcgg cagaccgaga
     2401 tcgacgacgg cgatgtcgag cggatattcc gagcctgcga acaggccttc ttcgccatcg
     2461 gccgcgacgt cgaccgtgaa cccctgcttg acgagttgtt cgcgcaggcc ttcgcgcagg
     2521 ctcgcttcgt cttcgaccac cagggcacgc atgcgatcag cgcatcctgc cggtagcggc
     2581 gtcgatgcgg acggtcacca cgcggccatc ttcagacagg agcttgagca catatacctt
     2641 gcggccgtct tcgtcctgta cttcggtgcg aaccacgcgc gcgcggtact gcgcctcggc
     2701 gcgccgtacg gcctcgtcca gcgaaatggc gt
//
LOCUS       DATDEU010000442         4042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1106086, whole genome shotgun sequence.
ACCESSION   DATDEU010000442 DATDEU010000000
VERSION     DATDEU010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106086"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VIV63_10575"
     CDS             complement(<1..996)
                     /locus_tag="VIV63_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125091.1"
                     /translation="MFGLRKRWAWLACAWLGGVAHAATFTGTIFEDEDYGGGAGRTLA
                     ASGGTVLPNVIVELYRADNGNWVATATTNASGVYSLSSGNTGAPMRVRVVNGSVRSAR
                     TGGAGCTTCVPVQTFRTDAITGAAVAVTNRVGGEAPGSSDAVVNPGSSNYSTLTAGGR
                     VPQSITSADPAANNSTISGIDFGFNFDTVVNTRDAAACGATNSSYPCQGSLRQFIINA
                     NALGGEGGLSQSGSGQIDGGTSFLPGGAESSIFMIPNAQLTGGVAVITLATALPAISG
                     GSTRLDATTQTVNIGNTNAGTLGTGGTVGVDAISLPTFPRPEVQLAAVNTVVTLSG"
     gene            complement(1175..1468)
                     /locus_tag="VIV63_10580"
                     /pseudo
     CDS             complement(1175..1468)
                     /locus_tag="VIV63_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            complement(1499..1870)
                     /locus_tag="VIV63_10585"
                     /pseudo
     CDS             complement(1499..1870)
                     /locus_tag="VIV63_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
     gene            complement(1921..2406)
                     /gene="folA"
                     /locus_tag="VIV63_10590"
     CDS             complement(1921..2406)
                     /gene="folA"
                     /locus_tag="VIV63_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HEY6125092.1"
                     /translation="MITLIVAVADNGVIGRDNALPWHLPEDLKRFKRLTMGKPMIMGR
                     KTFESIGKPLPGRLNIVVTRDTNYQREGVAVVHGAEAAIAAAVNAPEVMVIGGADLFR
                     LFLPMAGRVHLTRVHAEIHGNVMWPALDNRQWEVIEAERHEADERHAHAMTFEVWEKR
                     K"
     gene            complement(2403..2960)
                     /locus_tag="VIV63_10595"
     CDS             complement(2403..2960)
                     /locus_tag="VIV63_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY6125093.1"
                     /translation="MLDDFVDPQLLGDLRARCVELHDTGGLRPAKVGRGDREQHIPEV
                     RGDFIAWLQQPERDAEQRVLARLEELRASLNRALMTGLEDFQGHFALYPRGAGYARHF
                     DRLVGTDVRAISAVLYLNDAWQSDDGGQLRLYVGGGASVDVLPQGGRLVAFLSDRFEH
                     EVLPATRERLSFTGWFRRRPLEGFV"
     gene            complement(2990..3625)
                     /locus_tag="VIV63_10600"
     CDS             complement(2990..3625)
                     /locus_tag="VIV63_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY6125094.1"
                     /translation="MSKSKKTRKKKAKAFDQSQPKRGGPPIPTSPLVEVRNSDVHGRG
                     VFAVAPIAKGKRIFEYLGDRVSHDAADKRYEDHDENDNHTFLFIVDKNTVIDAAVNGS
                     DARFINHHCDGNCTSVIENRRVFIDSLRAIAPGEELGYDYEIGRDKDDSPNVDEIYAC
                     RCGSPKCRGSMLEAVAKPVKKKAAKRRKPGKSRKTRRPEAKRRGERPRRRA"
     gene            complement(3622..>4042)
                     /gene="thyA"
                     /locus_tag="VIV63_10605"
     CDS             complement(3622..>4042)
                     /gene="thyA"
                     /locus_tag="VIV63_10605"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY6125095.1"
                     /translation="RRIIVSAWNVGELDQMKLMPCHAFFQFYVSDKRLSCQLYQRSAD
                     IFLGVPFNIASYALLTHMLAQQCDLEVGEFIWTGGDCHLYSNHLEQVDEQLSRVPLTL
                     PKLVLTRRPPSMYDYEFEDFQIVNYQHHPAIKAPVAV"
BASE COUNT          671 a         1283 c         1328 g          760 t
ORIGIN      
        1 gccgctcaag gtgacgacgg tattgacggc ggcgagctgc acttcgggcc gcgggaaggt
       61 gggcagcgag atcgcatcga cgccgacggt gccgccagtc cccagcgtgc cggcgttggt
      121 attgccaatg ttcacggtct gggtcgtggc atcgaggcgc gtggaaccgc ccgaaatcgc
      181 gggcagtgcg gtcgcaagcg tgatcacggc aacgccgccc gtgagctgcg cgttcgggat
      241 catgaagatg ctggactcgg caccgcccgg caggaagctg gtgccgccgt cgatctggcc
      301 gctgcccgac tggctcagtc ccccttctcc gcccagcgca ttcgcgttga tgatgaactg
      361 gcgcagcgaa ccctggcagg gataactcga attggtggcc ccgcaagccg cggcgtcgcg
      421 cgtgttcacg accgtgtcga aattgaaacc gaagtcgatg ccgctgatcg tcgaattgtt
      481 cgccgcgggg tctgcgctcg tgatggattg cggaacgcgg ccgccggcgg tcagcgtcga
      541 gtagttcgag ctgccggggt tgaccacggc atcgctcgag cccggcgctt caccgccgac
      601 ccgattggtg acggccaccg cggcgcccgt gatggcgtcg gtacggaatg tctgaacggg
      661 cacgcacgtc gtacacccgg cgccgccggt gcgcgccgat cgcacggaac cgttgaccac
      721 gcgcacgcgc atcggcgcgc cggtgttgcc ggatgacagt gaatagacgc cactggcgtt
      781 ggtcgtcgcg gtggcgaccc agttgccgtt atccgcgcga tacaactcca cgatgacgtt
      841 cgggagcacc gtgccacccg acgcggcgag cgtccgcccc gcgccgccac catagtcctc
      901 gtcttcgaag atggtgccgg tgaaggtcgc ggcgtgagcc acgccgccca gccaggcaca
      961 ggcaagccag gcccaccgtt ttcgcagtcc gaacatggcg gccagtgtag ttcctgccct
     1021 gatcgggcat tcccttaatg gatcacattt tggccggcag aaacgtgcat cggcgcgcgg
     1081 ttctgcgtcg ccgtcgcccc aaacaaaaag gcgcgggagt tttcaccccc gcgcctttgt
     1141 ctggacaatt cggcagtcga actactgcgc gatcctattt cacgatctcg agcagctcca
     1201 cgtcgaacac cagcgtggac tgcggcggga tggtcggcgg cgagccgttg gcgccgtagg
     1261 cgaggttcgc cgggcaggtg agctgggcct tgccgcccac cttcatggcc tgcacacctt
     1321 cggtccagca ggcaatgacg ccgttcagcg ggaaggtcgc cggttcacca cgcgcgatcg
     1381 aactatcgaa gaccttgccg tcgatgaggc ggccttcgta gtgcaccttc acctggtcgg
     1441 tggcggccgg ggacgcgccc ttgccttcga ccgtgtgctt gatgagcatg ccgctcgccg
     1501 tcttcgtaac gcccggcgct ttcgacgcgg tgtcgaggaa agcgacgccg gccgcggcgg
     1561 ccttctcgga aaccacggcc atgcgcgcgc gctgcagttc ctgaactttc ggaatgtatt
     1621 cctcgggctt gatgtcgccc ttcacgccgg tcacgccgtc ggtcaggccc ttctgaacct
     1681 gcgcgagttc ttcgggcttg agatcgaaag tggagaggga ctggctcatc agcatgccga
     1741 gcgcgtacag cgtcttttgt tcttcggtca tgggagcttg ttccttcgcc ttcggtttgg
     1801 ccgcatcggc gtcgcagacg gtgagcgtga gggtgcagaa gatggccacg agggcagtta
     1861 gctttttcat agggcgccga gaatagcgcg cccaaagggc ctgcgggcgg gttttttcct
     1921 tcacttccgc ttctcccaca cctcgaatgt catggcgtgg gcgtgccgtt cgtcggcctc
     1981 gtgacgttcg gcttcaatga cttcccattg tcgattgtcc agcgcaggcc acatgacgtt
     2041 gccatgaatc tcagcatgaa cccgcgtgag atggacgcgc cccgccatgg gcaggaacag
     2101 gcggaacagg tcggcgccgc ctatcaccat gacttccggc gcattgacag ccgcggcgat
     2161 tgccgcctcg gcgccgtgca cgaccgcgac tccctcacgt tggtagttag tgtcgcgcgt
     2221 cacgacgatg ttcagccttc cgggcagggg tttgccgatg gactcgaaag tcttgcgccc
     2281 catgatcatg ggtttgccca tggtgaggcg cttgaagcgc ttgagatctt cgggaagatg
     2341 ccagggcaag gcgttgtccc ggcctatcac cccgttgtcg gcgacggcca cgatgagcgt
     2401 gatcacacga aaccctcgag cggacgccgc cggaaccagc ccgtgaaact caggcgctcg
     2461 cgcgtggcgg gcagcacctc atgttcgaaa cggtccgaca ggaacgcgac cagcctgccg
     2521 ccctgcggca acacgtcgac gctggcgccg ccgcccacgt acaggcgcaa ctgcccaccg
     2581 tcgtcgctct gccaggcatc gttgagatac agcacggcgg agatggcgcg gacgtcggtg
     2641 cccaccagac ggtcgaagtg ccgcgcgtag ccggcgccgc gcggatagag cgcgaagtgg
     2701 ccctggaagt cctcgagtcc ggtcatgagc gcgcgattga gcgaggcgcg cagctcctcg
     2761 agccgcgcga gcacgcgttg ctcggcatcg cgctccggtt gctggagcca ggcgatgaaa
     2821 tcaccgcgca cttcgggaat gtgctgctcg cgatcgccgc gcccgacttt cgcgggccgc
     2881 aggccaccgg tgtcgtgcag ttcgacgcaa cgcgcacgca ggtcgccgag caactgcggg
     2941 tcgacgaaat cgtcgagcac accgaagccg cgctcggcga gctggtcgat caggcccgcc
     3001 gtcgcggccg ttctccccga cgtttggctt cgggacgacg ggtcttgcgg gacttgcccg
     3061 gcttgcggcg cttcgctgct ttcttcttca cgggcttcgc caccgcctcc agcatggagc
     3121 cgcggcactt cggcgagccg caacggcagg cgtagatctc gtcgacgttc ggcgaatcgt
     3181 ccttgtcgcg gccgatctcg tagtcgtaac ccagctcttc accgggcgcg atggcgcgca
     3241 gcgaatcgat gaacacccgg cggttctcga tgaccgaggt gcagttgcca tcgcagtgat
     3301 gattgatgaa gcgcgcgtcg ctgccgttca ccgccgcgtc gatcacggtg ttcttgtcga
     3361 cgatgaacag gaacgtgtga ttgtcgtttt cgtcgtgatc ttcgtaacgc ttgtccgcgg
     3421 cgtcgtgtga aacgcggtca ccgagatatt cgaagatgcg tttgcccttg gcgatcggag
     3481 cgacggcgaa cacgccgcgt ccgtgcacgt cggaattgcg gacctcgacg agcggagacg
     3541 tcgggatggg cggaccaccg cgcttcggct gtgactggtc gaaagctttc gccttcttct
     3601 ttcgggtctt tttcgacttg ctcatacggc aacgggcgcc ttgatggccg ggtggtgctg
     3661 gtagttcacg atctgaaaat cctcgaactc gtagtcgtac atggatggtg gccggcgcgt
     3721 cagcaccagc ttcggcagcg tgagcggaac ccgcgagagc tgctcgtcca cctgttcgag
     3781 atgatttgaa tacagatggc agtcgccgcc ggtccagatg aactcaccca cctcgagatc
     3841 gcactgctgc gcgagcatgt gggtgagcaa tgcataagac gcgatgttga acggcacgcc
     3901 gaggaaaatg tccgcgctgc gctgatagag ctggcagctc aggcgtttgt ccgacacgta
     3961 gaactggaag aaagcgtggc aaggcatcag cttcatctgg tcgagctcgc ccacgttcca
     4021 ggcgctgacg atgatgcggc gt
//
LOCUS       DATDEU010000443         2723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1106298, whole genome shotgun sequence.
ACCESSION   DATDEU010000443 DATDEU010000000
VERSION     DATDEU010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106298"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1932
                     /locus_tag="VIV63_10610"
     CDS             37..1932
                     /locus_tag="VIV63_10610"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEY6125096.1"
                     /translation="MSQAGNATTNTQSSSRAVIDWRSFNIAPTESVAFVQPNAQAAVL
                     NRVTGSQFSSLQGSLTANGQVFLVNPNGILIGNGASINVGSFVASTANIGTTAFMRDS
                     AAANGRYAFDELANGARTATIVNAGNITVAEGGMVALVAPGVRNSGTITARLGTIELA
                     SATHFTLDVFGDDLVRLAVGDSIASGLVDSTGAALTAQVDAGGQLTADGGRVVLLTVP
                     AAAGVVSEAINLSGITRAQSVGVNQRGEISLLANQGAVRVSGRADASSTVAGVQGGEV
                     SMIGNSVQLTGVSRVDASGVGGGGRVVIGGSYAGDGATVTAQTTVDQGALVTACGTAS
                     CALDGAGGAGDGGEIRLFSTQGTSLGGEINVSSSASGRAGILEVVSDAGVTRLASMAR
                     LIGITGQDQLAGFTAIVGNTLELAPTAFVDMSDVAGNLPSGSNRLISDSRPGATTRSY
                     VSDPGLETRQTDQPVVIHAYINNGVSNYPDNLPAIRAAGVETPVGTLRPNGGAPTTFA
                     ATGADTLAAIPVTFTVPPPAGSDTITTQVANAANALTRDAVDSTQAIIDAGRNDTVGS
                     ASMLLVIGGPGVARVADLGRSGAVAGASPDVFGVNFHVLAPVGGADDAQISDYLCLTP
                     YARDACK"
     gene            complement(1952..2290)
                     /locus_tag="VIV63_10615"
     CDS             complement(1952..2290)
                     /locus_tag="VIV63_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125097.1"
                     /translation="MSNQQASRETESAIIPRRFAMKRSAEQYAVLSPLLDEALRLDDA
                     ARVVWLAKLPEEYASYRPALGRILTMDSAHSQSHLNGLELRLRSCSRGVGVLVLAEVD
                     RRKMGSDPIS"
     gene            2435..>2723
                     /locus_tag="VIV63_10620"
     CDS             2435..>2723
                     /locus_tag="VIV63_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125098.1"
                     /translation="MNSAQECLPSRLALAALSLLSGAALAADPYPQPQAYPQQQYPQQ
                     GGYPQQQYPQQQFQSQGPDPHPMRSLFASSLAQVAQTTGSTAVIMVADGLTG"
BASE COUNT          447 a          887 c          926 g          463 t
ORIGIN      
        1 cccaccgacg gcgtcgttcg cggccgagcg gcgacgatct cacaggcggg taacgcaacc
       61 acgaacaccc agagcagcag ccgcgcggtc atcgactggc ggtcgttcaa cattgcgccg
      121 actgagtcgg tagcgttcgt gcagccgaat gctcaggccg ccgtgctcaa tcgcgtgacg
      181 ggctcgcagt tctcgtcgtt gcagggctcg ctgacggcga atggtcaggt gttcctcgtg
      241 aaccccaacg gcatcctcat cggcaacggt gcgagcatca atgtcggatc gttcgtcgcg
      301 agcaccgcca atatcgggac aaccgcgttc atgcgcgata gcgcggcggc gaacgggcgc
      361 tatgcattcg acgagctggc gaacggcgcg cgcacggcga cgatcgtgaa tgccggaaat
      421 atcaccgtgg cggagggtgg catggtggcg ctggtggcgc cgggagtgcg caactccggc
      481 acgatcaccg cgcggctcgg caccatcgag ctcgcgtcgg ccacgcactt cacactggac
      541 gtgtttggtg acgatctcgt gcggctggca gtgggagatt cgattgcctc cgggctggtc
      601 gactctactg gcgccgcgct caccgcgcag gtggacgcgg gcggtcagtt gacggccgac
      661 ggcggccgcg tcgtattgct gacggtgccg gcggcggccg gtgtcgtaag tgaagccatc
      721 aatctatcgg gtattacgcg cgcgcaaagc gtgggcgtga atcagcgcgg cgagatctcg
      781 ctgctggcga atcagggcgc ggtgcgcgta agcggacgag cggatgcgag ctcgacggtt
      841 gcaggcgtgc agggcggtga ggtgtcgatg atcggcaact ccgtgcagct cacgggcgtt
      901 tcgcgcgtgg atgcctccgg cgtgggtggt ggcgggcgtg tcgtgatcgg cggcagttat
      961 gccggcgatg gcgccaccgt gacggctcaa actactgtgg atcagggcgc gctcgtgact
     1021 gcttgcggta cggcgagttg cgcgctggat ggagcgggcg gcgccggtga tggcggcgag
     1081 atacggctgt tctcgacgca gggtacgagc ctgggcggcg agatcaacgt gtcgtcgtcg
     1141 gcttcggggc gcgcgggtat cctcgaagtg gtgagtgatg cgggtgtcac gcgcctcgcg
     1201 tcgatggcgc ggctcattgg aatcacgggc caggatcagc tcgcgggatt taccgccatc
     1261 gtcggcaata cgctcgagct cgcgccgacc gcgttcgtgg acatgagcga cgtcgccggc
     1321 aatctgccgt cgggcagcaa ccggctcatc tcggattcgc ggccgggcgc cacgacgcgc
     1381 agttacgtgt cggatccggg cctcgagacg cgccagacag accagcccgt cgtcatccat
     1441 gcgtacatca acaatggtgt gtccaactac ccggacaacc tgccggcgat tcgcgcggcg
     1501 ggtgtggaga cgccggtggg cacgctgcgg cccaatggcg gcgcgccgac cacgttcgcg
     1561 gccaccggcg cggacacgct cgccgcgatt cccgtgacgt tcaccgtgcc gccgccggcc
     1621 ggcagcgaca cgatcacgac gcaggtggcg aatgccgcga atgcgttgac gcgtgacgcc
     1681 gtggattcga cgcaggccat catcgacgcc ggcaggaacg atacggtcgg cagcgcttcg
     1741 atgctgctgg tgatcggtgg tcccggcgtc gcgcgagtgg ccgacctcgg ccgttccggc
     1801 gccgtcgccg gcgcgagccc cgatgtgttc ggcgtgaact tccacgtgct cgcgcccgta
     1861 ggtggcgccg acgacgcgca gatctccgac tatctctgcc tgacccccta cgcccgcgac
     1921 gcctgcaaat aatggggtca gaccccattt gctaggaaat ggggtctgac cccattttcc
     1981 ggcggtcgac ctcggcgagc accagcactc cgacgccgcg gctgcagctt cgcagccgca
     2041 actccaggcc gttcaggtgg ctctgtgagt gcgcactgtc catcgtgagt atccgtccga
     2101 gcgccggccg gtaactcgcg tattcctccg gcagcttcgc cagccacacg acccgcgccg
     2161 catcgtcgag gcgcagtgcc tcgtcgagaa gcggagaaag cacggcgtat tgttccgcac
     2221 tgcgcttcat cgcgaaccgt cggggaatga ttgcggactc ggtttcacgg ctcgcctgtt
     2281 gattgctcat agaaacatac gctcgcacgc cagcccggcg gacatgcacg cggattcatt
     2341 ctcaccgcga gcgcatgtcc atccgcaccg cgaacttcgt atctaagtgt gaggcagcaa
     2401 atagctgtcg attccagcga atacaggtgc atcgatgaac agtgcccagg aatgccttcc
     2461 ctcacgtctc gcactggctg ccttgagcct gctgtccggc gcggccctgg ccgcggatcc
     2521 gtatccgcag ccccaggcct atccacagca gcagtacccc cagcagggcg gctacccgca
     2581 gcagcaatat ccgcaacagc agttccagag ccagggtccc gacccgcatc cgatgcgctc
     2641 gctgtttgcg tccagcctcg cgcaggtcgc gcagaccacc ggcagcaccg ccgtcatcat
     2701 ggtcgcggac ggactcaccg gcg
//
LOCUS       DATDEU010000444         5880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_373981, whole genome shotgun sequence.
ACCESSION   DATDEU010000444 DATDEU010000000
VERSION     DATDEU010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373981"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VIV63_10625"
     CDS             <1..357
                     /locus_tag="VIV63_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125099.1"
                     /translation="FIDWARELRMAGMRARIVAGLPGPASIRKLLQLARHCGVGPSIR
                     ALTARPGSLVKLLSDRDPGNLLNDLTAALQRQPELFDGVHLYSFGGFLRTATWLRHQA
                     ELQLEHRSFIRTPGAC"
     gene            complement(332..1387)
                     /locus_tag="VIV63_10630"
     CDS             complement(332..1387)
                     /locus_tag="VIV63_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6125100.1"
                     /translation="MIKQTIPLAALLLLAFQTGTATAQTQSEVPPPVSGAPPATLETI
                     KVHGRALEGNLEGNSADRDVLVFLPPGYRRERNRRYPVLYALHGFSIGAQQWSGEIHV
                     PQTIEGAFASGSREMIVVLPDSKTVHNGSMYSSSTTTGDFENFIARDLVAYIDSHYRT
                     LPRRESRGLAGHSMGGYGATRIGMKHADVFGALYIMSPCCLSVRAPPPAEVLSALADV
                     KTPADSAKLSFFARATLATAAAWSPNPGKPPLYLDLPVVDGQPQPDVLGKWAANSPLA
                     FADQYIGDLRRYSGIAIDVGDQDGLRTDTAKLQATLRRYGIASTFEEYKGTHTSAVAV
                     RFQNFVMPFFSKHLVFE"
     gene            complement(1384..2142)
                     /locus_tag="VIV63_10635"
     CDS             complement(1384..2142)
                     /locus_tag="VIV63_10635"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY6125101.1"
                     /translation="MAGERRAAPNRLRVHGIVAREIGISIVLGRLRPGHVLDGEIEAS
                     SQRRISRTAYREAIRILCAKGLIQSRPRTGTRVSEMESWNLLDPDVLAWVFSGVPKPE
                     VLHGLYELRSIVEPAAAALAATRRTQAHLTDMRRALDAMTLHTLDKVEGREADKAFHA
                     ALLAATNNPFVISLTNGVTAAVNALTEFKQRLAKIERDPVPDHLRVYDAIAAKDADGA
                     RQAMIKLIHLAVRDMPVKQRPRPPSFSTDPRGEK"
     gene            complement(2149..3717)
                     /locus_tag="VIV63_10640"
     CDS             complement(2149..3717)
                     /locus_tag="VIV63_10640"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6125102.1"
                     /translation="MTKNYLALAGAAAFFCILSTAAMAADEPAQIRIDASRPGATINR
                     HIFGQFAEHLGRGVYEGIWVGPSSPIPNTRGIRNDVVAALKAIRVPNVRWPGGCFADE
                     YHWRNGIGPRERRPATLNPNWGGVIEPNTFGTHEFMDFAEQIGADAYLSINVGSGTPA
                     EGADWLEYLTTPQPSALGKERAANGHPEPYRVPMLGIGNESWGCGGSMTPDYYVSQLK
                     IHARYARNYNTAQKMQRIAVGPDSKDTSYTKAVMEAWKNKTWAWDIEGISLHFYTVVK
                     WPPAFKATGFGETEYVTLLNETRRMDDLLRTHLAIMDQFDPEKKIAMVVDEWGAWLAP
                     TPGSPEGFLEQQNSQRDALIAALNINIFARHAERVRGANIAQMVNVLQAMILTNKERM
                     LLTPTYHVFRMYLPFQDATALPLTVSAGEYRHGDITLPRVDAIAARDAQGKTWLAVSN
                     LDPGHVAKVSIDVAGASYKRARGETLTAPKVDSVNTFDAPAVVAPKPFAAQAAGGKLS
                     LSLAPASITVVALE"
     gene            4134..5294
                     /locus_tag="VIV63_10645"
     CDS             4134..5294
                     /locus_tag="VIV63_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY6125103.1"
                     /translation="MAGTSVAQESAPFQPAETNVWGAEYPRIDSSGRVQIRIKAPEAN
                     KVKLNFWSGPKVDMEKQADGFWTVTTTPLVPGLHYYVINIDGADASDPGSKAYFGGSR
                     YTSAVEIPEPGSTYYSIQNVPHGQVRDVWYESKVTGSWRHAMVYLPPDYDAQTKKRYP
                     VLYLQHGGGEDETGWIRQGRANFILDNLIAAGEAKPMIIVMAYGYARRAGVPAPNLSG
                     AAMGTPQAAQAREEMAKAFEDDVTQVLIPFVDKTYRTLPDRDHRAMAGLSMGGFQTFH
                     ITTRHLDLFSHIGGFSGIGGMLEDRKVDLKTDFNGVFADPAAFAKKVHLLYFGVGTAE
                     AERFTTRIRGLHAALTEGGVEHVYWESPGTDHEWQTWRRNLKDFGSRIFRKK"
     gene            complement(5299..>5880)
                     /locus_tag="VIV63_10650"
     CDS             complement(5299..>5880)
                     /locus_tag="VIV63_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY6125104.1"
                     /translation="RYSVTYHVLRLAHRGTPTQTLTSAAAPLMQELSTQINHSCHLVV
                     RSGTQGLVILRQENQRRHANLSVRLGAVIELASSCSGQVLLAHLESAEREQLLQAIPR
                     PRGVSKSKVQNALDKVRKRGYEIHPSPITAGVTDISYPVRGFDGKVIAALTIPYLHAL
                     DNSLPTTVDKTRQLVEEAARRISQSLGWARAGG"
BASE COUNT         1069 a         1794 c         1874 g         1143 t
ORIGIN      
        1 ttcatcgact gggcccggga attacgcatg gccggaatgc gcgcgcgcat cgtggccgga
       61 ttgccaggac ctgcgagtat caggaagctg cttcagctgg cgaggcactg cggagtcggg
      121 ccatcgattc gcgcgttgac cgcgcggccc ggctcgttgg tcaaactgtt gtccgaccgg
      181 gaccctggca acctgttgaa cgacctgacg gctgcgctgc aacgccagcc cgaactcttc
      241 gatggcgtgc acctgtattc attcggcggg tttcttcgca ccgcgacctg gctgcgtcac
      301 caggcggagc ttcaactgga acaccgatcc ttcattcgaa caccaggtgc ttgctgaaga
      361 acggcatcac gaagttctgg aaacgcaccg ccaccgcact ggtgtgcgtg cccttgtatt
      421 cttcgaacgt gctcgcgatg ccgtagcgcc gcagcgtcgc ctgtagtttg gcggtgtctg
      481 tgcgcagccc gtcctggtcg ccgacgtcga tggcgatgcc ggaatagcgc ctgagatcgc
      541 caatgtattg atcggcaaac gccaacggtg aattcgccgc ccactttccc agcacgtcag
      601 gttgtggctg accgtctacc acgggcagat cgaggtacag aggcggcttc ccgggattcg
      661 gagaccaggc cgccgctgtt gccagcgtgg cgcgcgcgaa aaacgagagc ttggcggaat
      721 ccgccggagt cttcacgtcc gccagtgcgc tcagcacttc cgctggcggc ggcgcgcgca
      781 cggagaggca gcacggactc atgatgtaca acgcgccgaa cacatccgca tgtttcatgc
      841 cgatgcgggt cgcgccgtag ccgcccatcg aatggccggc gaggccgcgg ctctcacgcc
      901 tgggcaatgt ccggtagtga ctgtcgatgt atgccaccag gtcgcgcgcg atgaaattct
      961 cgaaatcgcc ggtggtggtg gagctcgaat acatcgagcc gttgtgcacg gtcttcgagt
     1021 ccggcaacac gacgatcatt tcgcgcgagc ccgatgcgaa cgcaccctcg atcgtctgcg
     1081 gaacgtgaat ttcgcccgac cattgttgtg caccaatcga aaagccgtgc agcgcgtaca
     1141 gcacgggata tcggcgattc cgttcccggc gatatccggg aggaagaaat accagcacat
     1201 ccctgtccgc ggaattccct tcgaggttgc cttcgagagc gcggccgtgg accttgatgg
     1261 tttcgagcgt cgcgggcggc gctcccgata ccggcggtgg aacctctgac tgcgtttgcg
     1321 ccgtggccgt accagtctgg aaagccagta gcagtaacgc ggcaagcggg atcgtttgct
     1381 tgatcatttc tcacccctgg gatcagtaga aaacgaaggc ggtcgcggtc tttgcttcac
     1441 cggcatgtcg cgcaccgcga gatgaatcag tttgatcatc gcctgccgcg cgccgtccgc
     1501 atctttcgcg gcgatggcat cgtagacgcg aaggtgatcg ggcaccgggt cccgttctat
     1561 tttcgccaat cgctgcttga actctgtcaa tgcgttgacc gcggcggtaa ctccgttggt
     1621 cagcgaaatg acgaagggat tgttagtcgc cgccagcaac gcggcatgaa atgccttgtc
     1681 cgcctcccgg ccctccacct tgtcgagcgt gtgcaaggtc atggcgtcga gcgcccgtcg
     1741 catgtccgtc aaatgcgcct gggtacgtcg tgtggcggca agcgcggccg ccgccggttc
     1801 gacgatgctg cgcagctcgt acaggccgtg cagcacttcc ggttttggca cgccgctgaa
     1861 cacccacgcg agcacatccg gatcgagcag attccacgac tccatctcgc tcacgcgagt
     1921 tccggttcgc ggccggcttt gaatcaggcc cttggcacag aggatgcgga tggcttcgcg
     1981 ataggcagtg cgcgaaatcc ggcgctggga actggcttcg atctcgccat cgagcacatg
     2041 accgggacga agccggccca acacgatcga aatgcctatt tcgcgcgcca cgatgccgtg
     2101 tacgcgaagg cgattcggcg cggcacggcg ctctccggcc acgccggctt actccagtgc
     2161 gacgactgtg atggaggcgg gcgccagcga cagactcaac ttgccgcctg cggcctgggc
     2221 cgcaaacggc ttcggtgcga ccaccgccgg cgcgtcgaat gtgttgacgc tatctacctt
     2281 gggcgccgtc agcgtttcgc cgcgcgctcg cttgtaactg gcgccggcga cgtcgatgga
     2341 aactttcgcc acgtggccgg gatcgaggtt cgaaaccgcc agccaggtct tgccttgcgc
     2401 gtcacgcgcg gcgatggcgt cgactcgcgg gagcgtgatg tcgccgtggc ggtattcgcc
     2461 ggccgaaacc gtgagcggca gggccgtggc gtcctggaat ggcaggtaca tgcgaaagac
     2521 gtgataggtc ggcgtcagca gcatccgctc tttgtttgtg aggatcatgg cctgcagcac
     2581 gttgaccatc tgcgcgatgt tggcgccgcg cacgcgctcc gcgtggcgcg cgaagatgtt
     2641 gatgttcaat gccgcgatga gggcgtcgcg ctggctgttc tgctgctcca gaaatccttc
     2701 cggactgccc ggagtcggag ccagccacgc gccccactca tcgaccacca tcgcgatttt
     2761 cttctccgga tcgaattgat ccatgatcgc gaggtgggtg cggagcaggt cgtccatgcg
     2821 ccgcgtctcg ttcagcagcg tgacgtactc ggtctcaccg aaacccgttg ccttgaacgc
     2881 gggcggccac ttcaccaccg tgtagaagtg gagcgagatt ccttcgatgt cccacgccca
     2941 ggtcttgttc ttccaggctt ccatcacagc cttggtgtag ctggtgtcct tgctgtcggg
     3001 cccgaccgcg atgcgttgca tcttctgcgc ggtgttgtag ttgcgcgcgt agcgcgcatg
     3061 gatcttgagc tggctcacgt aatagtcggg cgtcatcgag ccgccgcaac cccaggattc
     3121 gttgccgatg cctaacatgg gaacgcgata aggctccgga tggccattcg cggcgcgctc
     3181 cttgccgagt gcactgggct gcggggtcgt cagatattcc agccagtcgg cgccttcggc
     3241 cggcgtgccc gagccgacat tgatggaaag gtaggcgtcg gcgccgatct gttcggcgaa
     3301 gtccatgaat tcgtgcgtgc cgaatgtatt cggctcgatg acgccacccc agttgggatt
     3361 gagcgtggca gggcggcgtt cccgggggcc gatgccattt cgccagtgat attcgtcggc
     3421 gaagcagcca ccgggccagc gcacgttcgg aaccctgatt gccttcagcg cggccacgac
     3481 gtcattgcgg atgccgcgag tgttcggaat cggagaggag ggcccgaccc agatgccttc
     3541 gtagacaccg cggcccaggt gctcggcgaa ctggccaaaa atgtggcggt tgatcgtcgc
     3601 ccccggccgg gacgcgtcga tccggatttg tgccggctcg tcggccgcca tcgcggccgt
     3661 ggagagaatg caaaaaaagg cggcggcgcc ggccagcgcc agatagttct tcgtcacgag
     3721 tcccccttcg attcaagtcc gccacgcacg gtagcactga aaatgggctt tcactatggc
     3781 aagaaacttt cactaaacga atcggtgaat agcaaaacgt atattccagc ggaacgtgga
     3841 agtcattgga tttgctccgg aaacgaaatt ggccgggatt tccgcttgac aatgccggca
     3901 ggcgacatga gccttggcac cgcgcggatt gattagttag gcgggtcggg acctgggtgt
     3961 gtgcggcccc catcggttga gaggtgactt ctgttttccg cccgcgcctt ccagagcgca
     4021 gtcaccgtcg aggctgccgc gccggttccc cctgtacggc agcttcgatt ttttgcgcct
     4081 ggatccaaac gaacaggaga tctatgagaa cccttgtctg tgctttcttg ctgatggcag
     4141 gcacgtctgt ggcgcaggag tcggccccgt tccagccggc cgagacgaat gtctggggcg
     4201 cggagtatcc gcgcatcgat tcatcaggcc gggtgcagat ccgcatcaag gcgccggagg
     4261 ccaacaaggt aaagctcaac ttctggagcg gtccgaaggt ggacatggag aagcaggcgg
     4321 acggcttctg gacggtaacc accacgccat tggtgccggg cctccactac tacgtcatca
     4381 acatcgatgg cgccgacgcc agcgaccctg gcagcaaggc ctatttcggc ggttcacgtt
     4441 atacgagcgc ggtcgaaatt ccggaacccg gctcgaccta ctactccatc cagaatgtgc
     4501 cgcacggcca ggttcgggat gtgtggtacg agtcgaaggt cacgggctcc tggcgccacg
     4561 ccatggttta tctgccaccc gactacgatg cgcagaccaa gaagcgttat ccggtgctct
     4621 atctacagca cggtggtggc gaagacgaaa ccggctggat ccgccagggg cgggccaact
     4681 tcatcctcga caacctcatt gccgccggag aggccaagcc gatgatcatc gtcatggcct
     4741 acggctacgc gcgtcgagcc ggagttcccg cgccgaattt gtcaggggcc gcgatgggta
     4801 cgcctcaagc cgcgcaggcg cgcgaggaaa tggcgaaggc ttttgaagac gacgtgaccc
     4861 aggtgctgat cccgttcgtc gacaagacct atcgaacatt gccggaccgc gaccaccgcg
     4921 cgatggcggg gctatcgatg ggaggatttc agacctttca catcaccacg agacatctcg
     4981 acctgttttc gcacattggc ggattcagcg gcatcggcgg catgctcgaa gaccgcaagg
     5041 tcgatctcaa gacggacttc aatggcgtgt tcgccgaccc cgcggcattc gcgaaaaagg
     5101 tgcatctgct gtacttcggc gtaggtaccg cggaggcaga gcgattcacg acgcgtatcc
     5161 ggggtctgca tgccgcgctc accgagggcg gagtcgagca cgtctattgg gaatcaccgg
     5221 gaaccgatca cgagtggcag acgtggcggc gaaacctgaa ggatttcgga tcgcgcattt
     5281 tcaggaagaa atagccgact acccgccggc gcgcgcccaa cccagcgact gcgagatccg
     5341 gcgggctgct tcttccacga gctgccgggt cttgtcgacc gtggtgggca acgaattgtc
     5401 gagcgcgtgc aggtagggga tggtaagtgc cgcaatcacc ttgccatcga atccccgcac
     5461 cgggtagctg atgtcggtga caccggcggt gatgggactg gggtggatct cgtagccgcg
     5521 cttcctgacc ttgtcgagcg cgttctgcac cttcgatttc gagactccgc gcggacgagg
     5581 tatggcctgg agcaattgct cgcgttccgc ggactcgaga tgcgcgagca gcacctgccc
     5641 cgaacagctc gacgcgagct cgatgacggc gcccaacctg accgacagat tggcgtggcg
     5701 tctctggttt tcctgccgca ggatcaccag gccctgggtg cccgagcgca ccaccagatg
     5761 gcaggaatga ttgatttgag tagatagttc ctgcatcaat ggagctgctg ccgacgtcaa
     5821 ggtctgtgtt ggcgtgccgc gatgcgcgag cctcagcacg tgataggtca cgctgtagcg
//
LOCUS       DATDEU010000445         4249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1250833, whole genome shotgun sequence.
ACCESSION   DATDEU010000445 DATDEU010000000
VERSION     DATDEU010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250833"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1143)
                     /gene="pdxA"
                     /locus_tag="VIV63_10655"
                     /pseudo
     CDS             complement(127..1143)
                     /gene="pdxA"
                     /locus_tag="VIV63_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
     gene            complement(1095..2408)
                     /locus_tag="VIV63_10660"
     CDS             complement(1095..2408)
                     /locus_tag="VIV63_10660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6125105.1"
                     /translation="MIHKAFSRAFLVLLACAAAPAALSQTRETSSSGVLLDRVAATVN
                     EGVVLSSELDEQMLVITERLRGQKLDLPPQNVLRQQVLDRLVLQELQMQRADRAGIKV
                     SDETLNNALRDVAEQNKIPLAELPNALSSQGIDYASYRENIRKELAMQILRQRDVIGR
                     INVSPREIDQFLERQKKMPSESNEYNVSHILVAVPQAATPEELEEATKRADEVYTRAT
                     AGEDFGRLAVSFSNAQTALEGGSLGWRKGPELPSFLGEVIASMKTGDVTKPMRTPSGF
                     HIIKLNDMRGNAQVIVNQVHARHILIKPNELQDDATVQQKLVAIRDRILTKGENFTAV
                     ASVVSEDPGSSAEGGDLGWAGPGTYVPEFEKQLAQLQPDEISQPFRTQFGWHIIQLLG
                     RRQFDTTDDVRRQRAFMALREAKADEETELWLRRLRDEAYVEYKL"
     gene            complement(2420..>4249)
                     /gene="lptD"
                     /locus_tag="VIV63_10665"
     CDS             complement(2420..>4249)
                     /gene="lptD"
                     /locus_tag="VIV63_10665"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY6125106.1"
                     /translation="FEGTEFELPQNNARGSARKMDVDANGTLTLDDVSFTTCPANAVD
                     WRLNSRRIVLDSRARTGTGHGTSVEFKDVPVLYLPWMTFPIDDQRKSGFFFPSIGGSS
                     RNGAEVELPYYWNIRPNVDFMANPVYYGKRGVDFAGELRYLTQRQRGSLSFNFLPNDD
                     IEHIDRTRVQLEHVAELPGDWRFRIDATDVGDSRYFEDFAHGPEGTSIPFTERLAEAT
                     YRDENWNVRAQVQDFQTIDEDLAVEDRPYARTPRVLASGDWATGLGAIDYGFDAELVN
                     FERNTGVTGWRMDVAPRFGLDWSAPGFFVRPSGGFRYTQYSLEDNTPGTDSSPTRSLP
                     FASLDAGLVFERQAGSRGQRRVTLEPRALYLYTPFREQSDLPLFDTGLPDLNLVQLFR
                     ANRYVGADRVNDANQMAFGLTSRLFNADTGAQYLAASFGQSYYFEKPRVVLPDEPAAT
                     RDTSDFIAQVSLSAYKNWNVEAGIQWNPEDTRSERSQVRLQYRPAGDQVLNLSYRAQR
                     DVLEQAEVSGAWPLGKRWNAYARAVYSLRDSEMLERFAGFEYRSCCWRLRAVARRSIS
                     NREGTQESSFYLQLELNGLASVGDADAFLEHTIRGYSPETPVR"
BASE COUNT          677 a         1338 c         1492 g          742 t
ORIGIN      
        1 gacattcctg atttgcagca aaaggggacg cgcctcagtt taggttgagg cccgaggcgc
       61 gtcccctttt gcaagcaaat caggaatgtc cccatttccg gcctggcgcg aatccctgga
      121 cgcgaactcg agcgcgagtc gggtggcggc aatcaggctg ccggcatcgg cgcggccggt
      181 gccggcgagg tcgagcgccg tgccgtgatc taccgatgtg cgcacgatgg gcaggcccag
      241 cgtgacgttc accgcgtggc cgaagccggc gtgtttgagc acaggcagcc cctggtcgtg
      301 atacatggcc agcaccacgt cgtagttaga cagcgcacgc ggcacgaaca cggtgtcggc
      361 gggaatgggg ccatccacca tcatgccgct ggcgcgcgcg cgctcgatgg cgggcgcgat
      421 cacgtcgcgg tcctcggtgc cgagatgccc gctctcgccg gcatgcggat tgaggccgca
      481 caccgcgatt cgcggccgcg agatgcccca gaggcggcgc acgtcgtctt cgaggatccg
      541 cagcgtggta tcgagcagcg gcgccgtgat cgcatcgctg accgcgcgca gcggcaggtg
      601 cgtggtggcg agcgcgacgc gcagggaatc ggccgcgagc agcatgaccg gctgactcgc
      661 gccggtgcgc gcggccaggt attcggtgtg gccggtgaac ggcacgcccg cgtcgttgat
      721 gacgctcttc tgcaccggcg ccgtgaccat ggccgcgaac tcgccgctca ggcagccgtc
      781 ggtggcgcgg tcgagcagcg cgagaacgtg gcgcgaattg gcgggatcga ggcggcctgc
      841 cacgcagggc gcggcgagcg ggatgtcgag cacctgcagt cgaccggcct gcgcgccgcg
      901 cgacggagcg cccggtagat agggatcgag cgcgatgtcg agcccgagca tgtgcgcgcg
      961 ctcggcgagc agcgcacggc tgccgaggac tacgggcgtg gagggaagag caaggccggc
     1021 gagggcaagg cagaggtccg gcccgatgcc ggcgggttca cccgcggtga tggcaacggg
     1081 cttgtgggct gcgctcagag cttgtactcg acgtacgcct cgtcgcgcag gcgccgcagc
     1141 cacagttcgg tttcttcgtc ggccttcgct tcacgcagcg ccatgaacgc acgctggcgg
     1201 cgcacgtcgt cggtggtgtc gaactggcgg cggcctaaca actggatgat gtgccagccg
     1261 aactgcgtgc ggaacggctg gctgatttca tccggctgca gctgcgcgag ctgtttctcg
     1321 aactccggta cgtaggtgcc ggggccggcc cagccgagat cgccgccttc ggccgaggag
     1381 cccggatctt ccgagaccac cgaggcgacg gccgtgaaat tctcgccctt ggtgaggatg
     1441 cggtcgcgga tggccacgag cttctgctgc accgtggcgt cgtcctgcag ctcgttcggc
     1501 ttgatgagga tgtggcgcgc atgcacctgg ttgacgatga cctgcgcgtt gccgcgcata
     1561 tcgttgagct tgatgatgtg gaagccggac ggcgtgcgca tgggcttggt cacatcgccg
     1621 gtcttcatgc tggcgatgac ttcgccgagg aacgagggca gctcggggcc tttgcgccag
     1681 ccgagcgaac cgccttcgag cgcggtctgc gcgttcgaga acgacacggc cagccggccg
     1741 aagtcttcgc ccgccgtggc gcgcgtgtac acctcgtcgg cgcgcttggt ggcttcttcg
     1801 agctcttccg gcgtcgccgc ctgcggcact gccaccagga tgtgcgacac gttgtactcg
     1861 ttcgactccg agggcatctt cttctgccgc tcgaggaact ggtcgatttc gcgcgggctc
     1921 acgttgatgc ggccgatcac gtcacgctgg cgaaggattt gcatcgcgag ttccttgcgg
     1981 atgttttcgc gatagctggc gtagtcgatg ccctggctcg acagcgcatt gggcagctcg
     2041 gccaggggga tcttgttttg ctccgcgacg tcgcgcaggg cgttgttcag cgtttcgtcg
     2101 gacaccttga tgccggcgcg atccgcgcgc tgcatctgca attcctgcag caccaatcgg
     2161 tcgagcacct gttggcgcag gacgttctgc ggcggcaggt cgagtttctg cccgcgaagg
     2221 cgctcggtga tgaccagcat ctgctcgtcg agctcgctcg acagcaccac gccctcgttt
     2281 acggtggccg cgacccggtc cagcaacacg ccggagctgc tggtctcgcg ggtctgggat
     2341 aacgcggccg gagcggcggc gcaggccaga agaaccagga aggcgcgtga aaatgctttg
     2401 tgaatcatgt agttatattt caccggacgg gagtttccgg cgagtatccc cgaattgtgt
     2461 gttccaggaa agcgtccgca tctccgacac tggcaaggcc gttcagttcc agctgcagat
     2521 agaagctcga ctcctgcgta ccttcgcggt ttgaaatgga gcggcgcgcc acggcgcgca
     2581 ggcgccagca gcaggaccgg tattcaaaac ccgcgaagcg ctcgagcatc tcgctgtcgc
     2641 gcaagctgta cacggcgcgg gcgtaggcat tccagcgctt gcccagcggc caggcccccg
     2701 agacttcagc ctgttcgagg acgtcgcgtt gcgcgcggta cgacaggttc aacacctggt
     2761 cgcccgccgg ccggtattgc agccggacct gggagcgctc gctgcgcgtg tcttcgggat
     2821 tccactggat gcccgcctcg acgttccagt tcttgtaggc cgacagcgaa acctgcgcga
     2881 tgaagtccga agtgtcgcgc gttgccgccg gttcatctgg caacaccacg cgcggcttct
     2941 cgaagtagta gctctggccg aagctggccg cgaggtactg cgcgccggtg tcggcattga
     3001 acagccgcga ggtgagtccg aacgccatct ggttggcgtc gttgacgcgg tcggcaccca
     3061 cgtaacggtt ggcgcggaac agctgcacca ggttcaggtc gggaaggccg gtgtcgaaca
     3121 gcggcagatc gctctgctcg cggaacggcg tgtacagata gagcgcacgc ggctcgagtg
     3181 tcacgcggcg ctgcccgcgc gatccggcct ggcgctcgaa cacgaggccg gcatcgagtg
     3241 acgcaaacgg cagcgagcgc gtcggcgagg agtcggtgcc aggtgtgttg tcttcgagcg
     3301 aatactgcgt gtaacggaat ccgcccgatg ggcgcacgaa aaacccgggt gccgaccagt
     3361 cgaggccgaa gcgcggcgcg acgtccatgc gccagcccgt cacgccggtg ttgcgttcga
     3421 agttcacgag ctcggcgtcg aacccgtagt cgatggcgcc gaggcccgtc gcccagtcgc
     3481 cggaggccag gacgcgcggc gtgcgtgcat agggccggtc ttccaccgcc aggtcttcgt
     3541 cgatcgtctg gaaatcctgc acctgcgccc ggacattcca gttttcgtcg cgatacgtgg
     3601 cctcggcgag ccgctcggtg aacggaatgc tggtgccctc gggcccgtgc gcgaagtcct
     3661 cgaagtagcg gctgtcgccc acgtcggtgg cgtcgatgcg gaatcgccag tcgccgggaa
     3721 gctccgcgac gtgctcgagc tgcacacgcg tgcggtcgat gtgctcgatg tcgtcgttgg
     3781 gcaggaaatt gaaactcagc gagccacgct ggcgctgcgt gaggtagcgc aactcgccgg
     3841 cgaaatccac gccgcgtttg ccgtagtaga ccgggttcgc catgaagtcg acgttcgggc
     3901 ggatgttcca gtagtagggc agctcgacct cggcgccgtt gcgcgacgag ccgccgatac
     3961 tcggaaagaa gaaaccgctc ttgcgctggt cgtcgatcgg gaacgtcatc cacggtagat
     4021 agagaacggg cacgtccttg aactcgacgc tggttccgtg cccggtgccg gtgcgcgccc
     4081 gcgagtcgag cacgatgcgc cgcgaattca gccgccagtc gacggcattg gccgggcagg
     4141 tggtgaacga gacatcgtcg agcgtgagcg tgccgttcgc gtccacgtcc atcttgcgcg
     4201 ccgacccgcg ggcgttgttc tgcggcagct cgaactcggt gccctcgaa
//
LOCUS       DATDEU010000446         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1688463, whole genome shotgun sequence.
ACCESSION   DATDEU010000446 DATDEU010000000
VERSION     DATDEU010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688463"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1075
                     /gene="rpoA"
                     /locus_tag="VIV63_10670"
     CDS             71..1075
                     /gene="rpoA"
                     /locus_tag="VIV63_10670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY6125107.1"
                     /translation="MQGSVSEFLKPRVVKVQPTAPRQARVVIEPFERGFGHTLGNALR
                     RVLLSSMPGAAITEVEIEGVLHEYTSIEGVQEDVVDILLNLKQVALKMHTRDSAELRL
                     SKKGPGPVTAGDIHTDHDIEVVNPELVLANLTKAGELTMTLRVERGRGYRPALNRAAF
                     EEQSRPIGRLQLDASFSPVRRVTYMVDAARVEQRTDLDKLVIDIETNGTIDAEEAIRR
                     AGGILKDQLSVFVDLQGEEEASTKVSEMQFDPLLLRPVDELELTVRSANCLKAENIHY
                     IGDLVQRTEVELLRTPNLGKKSLTEIKEVLQSHGLMLGMRLDGWPPAGLKHDADRGMI
                     "
     gene            1099..1485
                     /gene="rplQ"
                     /locus_tag="VIV63_10675"
                     /pseudo
     CDS             1099..1485
                     /gene="rplQ"
                     /locus_tag="VIV63_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1739..>2066)
                     /locus_tag="VIV63_10680"
     CDS             complement(1739..>2066)
                     /locus_tag="VIV63_10680"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEY6125108.1"
                     /translation="EHDIRANYGHSFAERVAHDRQTPPALLLHGTTEAAAPLILRDGL
                     RPMKRQYVHMTPDRELALRVGARRGKPRIVKVAALAALEDGVVFYRANESFWLADSIP
                     PKYLST"
BASE COUNT          416 a          654 c          652 g          344 t
ORIGIN      
        1 ctcgtcgtcg agctctactc gaagtaattg agatttttct acccgcctcg cggcgggaag
       61 gtggaaacga atgcaaggtt cagtcagcga attcctcaag ccccgagtcg tgaaagtgca
      121 gccgacggcc ccgcgccagg ctcgcgtcgt gatcgaaccg ttcgagcgtg gcttcggcca
      181 cacgctcggc aacgcgctgc ggcgcgtgct gctgtcgtcc atgccgggcg cagccatcac
      241 ggaagtcgaa atcgaaggcg tgctccatga gtacacctcc atcgaaggcg tgcaggaaga
      301 cgtcgtcgac atcctgctca acctgaagca ggtcgctctc aagatgcaca cgcgcgacag
      361 cgccgagctg cgtctttcga agaagggccc gggcccggtg acggctggcg acatccacac
      421 ggaccacgac atcgaagtcg tgaacccgga gctggtgctc gcgaacctca cgaaggctgg
      481 cgaactcacc atgacgctgc gcgtcgagcg tggccgtggc taccgtccgg ccctgaatcg
      541 cgccgcgttc gaagagcaga gccgtccgat cggccgcctg cagctcgacg cgtccttctc
      601 gcccgtgcgt cgcgtgacgt acatggtcga tgcggctcgc gtcgaacagc gtaccgacct
      661 cgacaagctg gtcatcgaca tcgagaccaa cggcacgatc gacgcggaag aagccatccg
      721 tcgcgccggc ggcatcctca aggatcagtt gtcggtgttc gtggacctgc agggtgagga
      781 agaagcctcg accaaggtct ccgagatgca gttcgatccg ttgctgctgc gcccggtcga
      841 cgagctcgag ctcaccgtgc gttcggccaa ctgcctcaag gccgaaaaca ttcactacat
      901 cggcgacctc gtgcagcgca ccgaagtcga gctgcttcgc acgccgaacc tcggcaagaa
      961 gtcgctcacc gagatcaagg aagtgctgca gagccacggc ctcatgctcg gcatgcgtct
     1021 cgatggctgg ccgccggccg gtctcaagca cgatgccgat cgtggaatga tctaagacaa
     1081 tagttaggga acagtttcat gcgtcatcat ctttccggac gtcagctgtc gcgcaatgcg
     1141 ccgcatcgcc acgcgttgct gcgtagcctg tcggtgtcgc tgctgcgtca cgagaccatc
     1201 cgcaccacca tcccgaaggc caaagagctt cgccgcgtgg tcgagccgtt gatcaccctc
     1261 ggcaaggacg acagcgaagc caatcgccgt ctcgcgttct cgcgcctgcg cgacaccgag
     1321 atcgtcgcca agctgttcga caccatcggc ccgcgcttca aggcgcgtcc gggtggctac
     1381 acgcgcatcc tgcacatggc gccgcgtccg ggtgacaacg ccccgatggc gctgatgcag
     1441 ctcgtcgagc gctccgcgcc ggtggccgtc gaagccgcgc ctgaagacaa gaaggcgaag
     1501 aaggtcgagg cagctccgaa ggtcgacaag gccgagaaga aggccaaggc cgaagccaag
     1561 aaaggccgcg aaaaggccgc caaggaagcc aacgctgcca aggctgtcgc gaagaaggcc
     1621 gccaagaaga ccggcggcgc ggccaagaag aaaaaagctg ctccgaagaa gaagaaatct
     1681 tcgaagtagt tcgtctagta gatgcaaaac gggcgcctca gtggcgcccg ttttcgtttc
     1741 aggtgctcag atatttcggc gggatcgaat ccgcgagcca gaaggattcg ttcgcgcgat
     1801 agaagacgac gccgtcctct agtgccgcga gcgcggccac tttcacgatc ctgggtttgc
     1861 cgcgcctcgc accgactcgc agcgcgagtt cccgatcggg cgtcatgtgc acgtactggc
     1921 gtttcatggg ccggagcccg tcacgcagga tgagcggcgc cgccgcttcc gtggtgccgt
     1981 gaaggagtaa tgcgggcggc gtctgtcgat cgtgtgcaac gcgttcggcg aacgagtggc
     2041 cgtagttggc gcggatatcg tgttcg
//
LOCUS       DATDEU010000447         4549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_814289, whole genome shotgun sequence.
ACCESSION   DATDEU010000447 DATDEU010000000
VERSION     DATDEU010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814289"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VIV63_10685"
     CDS             complement(<1..986)
                     /locus_tag="VIV63_10685"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125109.1"
                     /translation="MQTNILEAAREIVPLITRHRGETESARRIAQPVVEALRQSRLCR
                     LAIPRELNGLEMPTPAALEIYETLAAAEASVGWIVWNNTLPCFWGRFLQPAVRAEIFG
                     NPEWLHASSTRPSGKAILEGDGYRVTGRWSLVSGCELSEWMALRCMVEENGQPRMLQP
                     GVPEARMIFLHRTDVEILDTWHTGGLRGTGSHDVTVTNKLIARAHTCSPMEGSTLAGP
                     IGCVPIVCNMAAGYGAQLLGLGSAALDVLIELSRSKPVVDPGPSLGERPAVLAAIAEH
                     RARLAAAREFLHSRVTRLWKEAEAGRRPTDRIAEVYAAAHHALAQGRAAVSTA"
     gene            complement(1020..2447)
                     /locus_tag="VIV63_10690"
     CDS             complement(1020..2447)
                     /locus_tag="VIV63_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase-like domain-containing
                     protein"
                     /protein_id="HEY6125110.1"
                     /translation="MKTPMLLLSVFILALSSVAAAAAKPRVFVLTDIENEPDDAMSMV
                     RFLVYSNQWDVEGLAATTSVHQKNKVAAWRIREIVDAYAKVRDNLELHEPGFPDADAL
                     RAVIVEGRPDFGMHAVGKAMDSKASELLIQAADRKDSRPLWVPVWGGPNVLAQALWKV
                     RATRSPQELRRFVAKLRVYTISDQDDSGPWIRKTFPELFYIASPGVHGGGAYHYSTWS
                     GISGDKFHGRFTGADFSIVDNPWLDKHIRAKGPLGEQYPQVKFLMEGDTPSFLNLIGN
                     GLSDSEHPDWGGWGGRYENYTPRMRKWFDEPETRPFWSDAEDEVLGVDGNWHTSNHAT
                     IWRWREAYQNDFVARMDWTIKPRAEANHPPVARLAHADALDAKAGATVALSAEGSTDP
                     DGNALTYLWFYYPEPGTLSVATGRSGAPVAIDKANEREATLVVPKNFPKAGTMHFILA
                     VTDNGTPALTRYRRVVVSVRGPADP"
     gene            complement(2458..3954)
                     /locus_tag="VIV63_10695"
     CDS             complement(2458..3954)
                     /locus_tag="VIV63_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6125111.1"
                     /translation="MKRRDFLNTSLFAAAVSVPGIRAAYAVVGNDPVADLAAITGDGR
                     EITLRGADIRDLAAKLRGQLLIAGNDGYDKARLVLNPSFDKHPALIAQPTGAADVQAA
                     VNFARAHSLLVAVKCGGHSASGQSTCDKGMMIDLSNFRGVRVDPKARRAFVEGGTLLG
                     HVDHESMAHELVAPLGTVSHTGVGGLTLGGGFGRLARKFGMSIDNVESMDVVTADGQL
                     RHASASENPDLYWALRGGGGNFGVVTNFEFRLHPMKREVIAGSVRFPIARARDVLAMY
                     ADYAAAAPDELYMDPVIMAPPGGAPGTVSVEVCYCGPQENAERALAPLRKLGTPERDS
                     IKVNDYVRVQRWNDSGDSRALGSYLKGGFISQVPDKLVSAIVDNFQPDPGRMTLLFFQ
                     HCGGASSRPAESATAFAQRDTLANMMTVTGWRQGVDDPAAPIDAARRYWKTLEPFTRG
                     FYVNDLAREVTARDINENYRGNYPRLVKIKKAYDPTNLFRLNANVQPA"
     gene            complement(4048..>4549)
                     /locus_tag="VIV63_10700"
     CDS             complement(4048..>4549)
                     /locus_tag="VIV63_10700"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEY6125112.1"
                     /translation="RDAVDFLALGATTVQVCTAAMVYGFKIIDDLVDGLNNYLDTKGF
                     ASVSEIVGKAVPSVTDWQYLNLNHVDKAFIDQDLCIKCGRCHIVCEDTSHQAITQTVN
                     GARHFEVIDAECVGCNLCVSVCPVENCITMQPMTSGTDPRTGRAITNVKADWTTHPNN
                     PGRKNA"
BASE COUNT          749 a         1463 c         1489 g          848 t
ORIGIN      
        1 gccgtggaca cggcggcgcg gccctgcgcc aaggcgtgat gcgcggccgc gtaaacttcc
       61 gcgatcctgt ccgtgggtct tcgccccgcc tcggcttctt tccagaggcg cgtgacccgc
      121 gaatgcagga attcgcgtgc cgcagcgagt cgcgcccgat gttcggcgat ggctgcaagc
      181 actgccggcc gttcgccaag tgacggcccc ggatcgacga ccggcttgct gcgggacagt
      241 tcgatcaaca cgtcgagtgc cgcgctgccg agccctaaca actgcgcgcc gtatccggcg
      301 gccatgttgc aaacgatggg cacgcagccg atggggcccg ccagagtgct gccttccatc
      361 ggtgaacacg tgtgggcgcg ggcaatgagc ttgttcgtga cggttacatc gtgacttccc
      421 gtgccacgca gtccgcccgt atgccacgta tcgaggatct cgacatccgt acggtgcagg
      481 aaaatcatgc gggcctcggg gacccccggc tgcaacatgc gtggctggcc gttttcttcg
      541 accatgcagc gcaatgccat ccactcggac aactcgcaac ccgacaccag tgaccagcgt
      601 cccgtgacac ggtagccatc gccctcgagt atggccttgc cactggggcg cgtcgaactc
      661 gcatgaagcc actcgggatt gccgaagatt tcagcgcgca cggcaggctg caggaaccgc
      721 ccccagaaac agggcagcgt gttgttccag acgatccagc ccaccgaagc ctcggccgcc
      781 gcgagtgttt cgtagatttc gagcgccgcg ggcgtgggca tctccaggcc attcagctcc
      841 cggggaatcg ccaggcggca caggcgcgac tggcgcagcg cttcgaccac gggctgagcg
      901 atgcgccgcg cggattcggt ttcgccgcga tgccgcgtga tcagcggcac tatctcccgt
      961 gccgcttcga gaatattcgt ttgcatgagc ccgatgttac ggctcgccgc gcgcgcggct
     1021 caagggtccg ctggccctcg cacggaaacc acgacgcggc gatagcgcgt gagcgccggt
     1081 gtcccgttgt cggtcaccgc gaggatgaag tgcatcgtgc ccgctttcgg gaaattcttc
     1141 ggcacgacga gcgtggcctc gcgttcgttg gccttgtcga tggccacggg cgcgccggag
     1201 cgacccgtcg ccacgctcag cgtgcccggc tccgggtagt agaaccatag ataggtgagt
     1261 gcattgccgt cgggatcggt cgacccttcc gcgctcaatg caacagtggc gccggccttg
     1321 gcgtcgagcg catccgcgtg cgccaggcgc gccaccggcg gatggttcgc ctcggcgcga
     1381 ggtttgatcg tccagtccat gcgtgccacg aaatcattct ggtacgcctc gcgccagcgc
     1441 cagatcgtgg cgtggttgga cgtgtgccag ttgccatcga cgcccagtac ttcgtcttcg
     1501 gcatccgacc agaagggccg cgtttcgggt tcgtcgaacc acttgcgcat gcgcggcgtg
     1561 tagttttcgt atcgtccacc ccagccgccc caatcgggat gttcggaatc actcaggcca
     1621 ttgccgatga ggttgagaaa actcggtgtg tcgccttcca tgaggaactt gacctgtggg
     1681 tactgttcgc cgagcggacc cttggcgcga atgtgcttgt cgagccaggg attgtcgacg
     1741 atggaaaaat ccgcacccgt gaaccgtccg tggaatttgt cgccgctgat gccgctccac
     1801 gtggagtagt gatacgcgcc gccaccgtgt acaccagggc tcgcgatgta gaagagttcg
     1861 ggaaaggtct tgcgtatcca cgggccgctg tcgtcctgat cggagatcgt gtagacgcgc
     1921 agcttcgcga cgaacctccg cagctcctgg ggtgaacgcg tggcgcgcac tttccacaga
     1981 gcctgggcga gcacgttcgg gccgccccac acgggaaccc acaggggccg ggagtccttg
     2041 cggtctgccg cctggatgag tagttcggaa gccttcgagt ccatggcttt gccgaccgcg
     2101 tgcatgccga aatcgggacg accctcgaca atgacggcac gcagcgcgtc cgcgtccggg
     2161 aagccaggct catggagctc gaggttgtcg cgcaccttcg catacgcatc gacgatttcg
     2221 cggatgcgcc aggccgccac cttgttcttc tgatgcacgg aggtcgtcgc cgccaggcct
     2281 tcgacatccc attggttcga gtaaacgaga aaccggacca tcgacatggc gtcgtcgggt
     2341 tcgttctcga tgtcggtaag cacgaacacg cgcggcttcg ctgccgcagc cgctacgctg
     2401 ctcagggcga gaataaagac gctcagcaac agcatcggcg ttttcatgcg cgcgagctca
     2461 cgccggctgg acgttcgcgt tcaaccggaa caggttcgtg gggtcgtacg ccttcttgat
     2521 cttcacgagc cgcgggtaat ttccgcggta gttctcgttg atgtctctgg cggtgacctc
     2581 gcgcgccagg tcgttgacgt agaacccgcg cgtaaagggt tcgagcgtct tccaatagcg
     2641 gcgcgccgcg tcgatgggcg cggcggggtc gtcgacgcct tgccgccagc cggtcacggt
     2701 catcatgttc gcgagtgtgt cgcgttgcgc gaaggcggtg gcgctctccg cgggacgcga
     2761 gctggcgccg ccgcaatgct ggaagaacag cagcgtcatg cggcccgggt ccggctggaa
     2821 gttgtcgacg atggccgaga cgagtttgtc gggcacctgc gagatgaagc cgcctttgag
     2881 gtatgaaccg agcgcacgcg aatcgcccga gtcgttccat cgttgcacgc ggacgtaatc
     2941 attgaccttg atggagtcgc gctccggtgt acccagcttg cgaagtggcg cgagcgcgcg
     3001 ttccgcattt tcctgtggcc cgcaataaca aacctccacg gacacggtgc cgggcgcgcc
     3061 acccggaggc gccatgatga cggggtccat atacaactcg tccggcgccg ccgccgcata
     3121 gtcggcgtac atcgcgagga cgtcgcgtgc gcgcgcaatc ggaaaccgca ccgagcccgc
     3181 gatgacttcg cgcttcatcg ggtgcagccg gaactcgaag ttcgtcacca cgccgaaatt
     3241 gccaccgcca ccgcgcaatg cccagtacag gtccgggttt tcgctggcgc tcgcgtggcg
     3301 cagctgcccg tcggcggtga cgacgtccat ggattcgacg ttgtcgatcg acatgccgaa
     3361 cttgcgagcc agtcgcccga atcctccgcc cagcgtgagg ccgcccacgc ccgtgtgcga
     3421 gaccgtgccg agcggcgcca cgagctcgtg ggccatgctc tcgtggtcca catgtcctaa
     3481 caacgtgccg ccctcgacga aggcgcgccg ggccttcggg tccacgcgca cgccgcggaa
     3541 gttcgacagg tcgatcatca tccccttgtc gcaggtcgat tgtcccgagg cgctgtggcc
     3601 gccgcacttc accgccacca gcagactgtg tgcgcgcgcg aaattcaccg cggcctgcac
     3661 gtcggccgcg cctgtgggtt gcgcgatgag ggcagggtgt ttgtcgaaag aggggttgag
     3721 gaccaggcgc gccttgtcgt acccgtcgtt gcccgcgatc agcagctggc cgcgcaactt
     3781 tgcagcgagg tcgcggatat cggcgccgcg cagcgtgatc tcgcgtccat cgcctgtgat
     3841 ggcggcgagg tccgcgaccg gatcgttgcc aactaccgcg taggcggcgc ggatccccgg
     3901 aacgctgacg gcggcggcga acagcgacgt gttcaggaag tctcggcgtt tcatggttca
     3961 gcctcactct tggtggtgat tgcggagccc ggcgaatatc tcatgtccgc cgctttgccg
     4021 cagcagatcc gcgcaagcgc tgacgcctca ggcgttcttg cgacccggat tgttagggtg
     4081 cgtggtccag tcggctttca cgttcgtgat ggcacggccg gtgcgcggat ccgtgccgct
     4141 ggtcatcggc tgcatggtga tgcagttctc caccgggcag acgctgacgc acagattgca
     4201 gcccacacac tcggcgtcga tgacttcgaa atgccgcgcg ccgttcacgg tctgcgtgat
     4261 ggcctgatgc gacgtgtctt cgcagacgat gtggcatcgg ccgcacttga tgcacagatc
     4321 ctggtcgatg aacgccttgt cgacgtgatt gagattgagg tactgccagt cggtgacgct
     4381 gggcacggcc ttgccgacga tctcgctcac ggacgcgaag cccttggtgt cgagatagtt
     4441 gttcaggccg tccacgaggt cgtcgatgat cttgaagccg tagaccatgg cggccgtgca
     4501 cacctgcacg gtcgtggcgc cgagcgcgag gaaatcgacg gcatcgcgc
//
LOCUS       DATDEU010000448         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1834466, whole genome shotgun sequence.
ACCESSION   DATDEU010000448 DATDEU010000000
VERSION     DATDEU010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834466"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2335)
                     /locus_tag="VIV63_10705"
     CDS             complement(<1..2335)
                     /locus_tag="VIV63_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY6125113.1"
                     /translation="MTEPRIRNDDAKPLIAGLKAPLARSPTREYIAKVVERLHREVRY
                     TGVEFAEARLVPEYPAETLRRRFGDCKDKSTLLIAALRASGIDAYLALLDSGDDQDVS
                     PDLPGLGMFNHAIVYVPGTPDIWIDATAEYTRVGSLPPPTTDRLALIIRAGEKTLTRT
                     PAMRSVDNAQVETRDFFLAEYGPARVIETTETHGAVEGEFRSWYAGADNKARIDDLTS
                     YARSAYRAKSLVNYEHTPSTDFSKPYSMSIEMKDAPVGFTDLHSSAVGVSISNITSRL
                     PSYFDDRLEEDSDGKESSTRTADVVFEPFVTEWNYRIRPPPGFQARKLPDDGVQSLGP
                     AQLKSEFKLSADGAVHAMWRFDTVKRRYTPAETDALLKALRELKSAETQLITFDQVGV
                     ALRAEGDFKGALHANDALIVAHPRKAVHRLRAATALLEAGLGARAQREALAATKLEPK
                     SALAWKTLGWMLQHDAVGRRFGEGFDRDASLAAYQKARALEPENTDIAADLAVLLEHD
                     ANGVRYAPGANLEAAISLYRLRREQLSEEDAGDDKYTRNLSYALFYARRYADLREELR
                     KQSPGATQRALTLAAIAAEGGAGSGSAKALELARGFSGDESDRRTALTSAGNLLVRVR
                     EYSSAADLLEASTRGQATTAATTQRIATLRKTQPNDGKAIEPTDPRGVVLRWFAELLA
                     VDSDDGGLRRLLVPGSEALLESTDFAQARRLVVTGLRKQDLPFEVGADLLFSNLRVNV
                     EGDDAGGYRVQLRMTGETQSFFVVKLDGGYRILAIPGQ"
BASE COUNT          357 a          837 c          809 g          431 t
ORIGIN      
        1 gctgccccgg tatcgcgagg atgcgatagc cgccatcgag cttcaccacg aagaagcttt
       61 gcgtttcgcc cgtcatgcga agctgcacgc ggtacccgcc ggcgtcgtcg ccttcgacat
      121 tcacgcgcag attgctgaac agcaggtccg cgccgacctc gaacggaaga tcctgcttcc
      181 gaagacccgt gaccaccagg cgccgcgcct gggcgaagtc cgtgctttcg agcaacgctt
      241 cgctgcccgg tacgagcaac cggcgaagcc ccccatcgtc actatctacc gccagcaact
      301 ccgcgaacca gcgcagcacc acaccgcgcg ggtccgtggg ctcgatcgcc ttgccgtcgt
      361 tgggctgcgt cttgcgcaag gtcgcgattc gctgcgtggt ggccgcggtc gtcgcctggc
      421 cgcgcgtgct cgcttcgagg aggtcggccg cgctcgagta ttcgcgcacg cgcaccagca
      481 ggttgcccgc gctggtgagc gccgtgcgcc ggtccgattc gtcgcccgag aacccgcgcg
      541 cgagctcgag cgccttcgcg ctgccgctcc cggcgccgcc ctcggcggca atcgctgcga
      601 gcgtcaacgc gcgctgcgtg gcccccggcg actgcttgcg caattcctcg cgcaggtccg
      661 cgtagcgccg cgcgtagaac aacgcatacg aaagattccg cgtgtacttg tcgtccccgg
      721 catcttcttc gctcagttgc tcgcgccgca gccggtagag gctgatggcc gcttcgagat
      781 tcgcgccggg cgcataacgc acgccgttgg cgtcgtgttc gagcagcacg gcgagatccg
      841 ccgcgatatc ggtgttctcg ggctcgagcg cgcgcgcctt ctgatacgcc gcgagcgacg
      901 cgtcgcggtc gaacccttcg ccgaaccggc gacccacggc gtcatgctga agcatccagc
      961 ccagcgtctt ccacgcgagc gcggacttgg gctccagctt cgtcgcggcc agcgcctcgc
     1021 gctgtgcacg cgcgcccaat ccggcttcga gcagcgccgt cgccgcgcgc agccggtgta
     1081 ccgccttgcg cgggtgcgcg acgatcaacg cgtcgttggc atgcagcgcg cccttgaaat
     1141 ctccctcggc acgcagcgcg accccgacct ggtcgaacgt gatgagctgc gtctcggcgc
     1201 tcttgagttc gcgcagcgcc ttgagcagcg cgtcggtctc ggccggtgtg tagcggcgct
     1261 tcacggtgtc gaagcgccac atggcatgca ccgcgccatc cgccgacaat ttgaattcgg
     1321 acttgagctg cgccggcccg aggctctgca cgccgtcgtc cggcagcttg cgcgcctgga
     1381 aacccggcgg cgggcggatg cggtagttcc actcggtgac gaaaggctcg aacaccacgt
     1441 ccgcggtgcg cgtcgacgac tccttgccgt cggagtcttc ttcgagccgg tcgtcgaagt
     1501 acgagggcag ccgcgaagtg atgttcgaga tgctcacgcc gacagccgaa gaatgcaggt
     1561 ccgtgaaacc cacgggcgca tccttcattt cgatgctcat ggaatagggc ttcgaaaaat
     1621 cggtgctggg cgtgtgctcg taattcacca gcgacttggc gcggtaggcg ctacgcgcgt
     1681 aactggtcag gtcgtcgatg cgcgccttgt tgtcggctcc cgcataccag ctgcggaatt
     1741 cgccttcgac ggcgccgtgc gtttcggtgg tttcgatgac gcgagccggg ccgtactcgg
     1801 ccagaaagaa gtcgcgtgtc tcgacctgcg cattgtcgac cgagcgcatg gcgggcgtgc
     1861 gcgtgagcgt tttttcgccg gcgcgaatga tcagcgccag ccggtccgtg gtcggtggcg
     1921 gcaggctgcc cacgcgcgtg tattccgcgg tggcgtctat ccagatgtcg ggcgtgcccg
     1981 gcacgtagac gatggcgtgg ttgaacatgc cgagaccggg caggtcgggc gacacgtcct
     2041 ggtcgtcgcc cgaatcgagc agcgcgagat aggcgtcgat gcccgacgcg cgcagcgccg
     2101 cgatcagcag cgtggacttg tccttgcagt cgccgaaccg gcggcgcagc gtctcggcgg
     2161 gatattccgg aaccagccgc gcctcggcga actccacacc ggtgtagcgc acctcgcgat
     2221 gcaggcgttc cacgaccttc gcgatgtact cgcgcgtcgg gctgcgggcg agcggcgcct
     2281 tgagcccggc aatcagtggt ttggcgtcgt cgttgcggat gcgcggctcg gtcatctcgc
     2341 ggtacgtcgc cgccaccgct tcccacgagg tggccgacgt gaattccacc atcggccacg
     2401 atggcacgtc cggcggcagg ttcggttcca tctc
//
LOCUS       DATDEU010000449         7610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1543295, whole genome shotgun sequence.
ACCESSION   DATDEU010000449 DATDEU010000000
VERSION     DATDEU010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543295"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /gene="rpoD"
                     /locus_tag="VIV63_10710"
     CDS             <1..1995
                     /gene="rpoD"
                     /locus_tag="VIV63_10710"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY6125114.1"
                     /translation="KPAQKLAAARAAAAATVAAPVEGKPGKGKKGSAELINLPARRNV
                     LPEERQSQLKLLIARGKEQGYLTYSQVNDHLPSEIVDPEQIEEIVNTINDMGIPVYEK
                     APDNESILAPEPSAPTDEEAIEEAAAALAALDAELGRTTDPVRMYMREMGTVELLTRE
                     GEIRIAKRIEEGLDAVRLALSNYPSTSEYLLRAYEPVKTGGARLVDIIVGFIDPNAPD
                     VIAQPQNPTKMDLAAPVPEEKPEGEETEEGDGTGEEEEAVDTGPDPEEAARRFAAVGK
                     YLANSLTHIEKMGAKDPKTLKVRKRLSDEFMELKLAPRMFEALIGNLRNQVNQIRQLE
                     KEIMIIAVRDAGMPRKEFILSVPKNETSNRWLQKQVKGGKKWSNALGRLQDEISRRQE
                     KLSTLEKEYHLTITDIKEINREVSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQ
                     FLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIE
                     TINKLNRISRQMLQEMGREPTPEELAVRMEMPEDKVRKVLKIAKEPISMETPIGDDED
                     SHLGDFIEDTSVASPIDQATMESLRETTHSVLAQLTPREAKVLRMRFGIDMNTDHTLE
                     EVGKQFDVTRERIRQIEAKALRKLRHPSRSEQLRSFLIED"
     gene            2168..2791
                     /locus_tag="VIV63_10715"
     CDS             2168..2791
                     /locus_tag="VIV63_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125115.1"
                     /translation="MPDGLEMSGYGPDRHPGEANKFREGYLWNELQREDAALAAAIAA
                     QGHCTVLRADLEDQPTLNYLRDTIGIATYLLDHGCVAIYDPQMICYWSPSRWRSKVFD
                     PAAAVPRNHVMILYDGDGEGTEWFHTRGMRKFGRPDLSVPRTPAKHRDAVIDLINRFI
                     EYQAFGGVIEEGREIKVNDLPAGMRCSHHGNLDDPDFNNVHVRIDWS"
     gene            complement(2792..3562)
                     /locus_tag="VIV63_10720"
     CDS             complement(2792..3562)
                     /locus_tag="VIV63_10720"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY6125116.1"
                     /translation="MFKRLSIFLYGVVSYAVFFLTFLYAIGFVGNFAVPRTLDGAPAS
                     DLTTALLVNLGLLGLFAIQHSVMARPAFKRWFTKLVPESAERSTYVLLSSVALIAMFY
                     GWQPLGGVVWTVTDATARGVIWGCFAFGWLLVLVSTFLINHFDLFGLRQVWLQLMGRP
                     YTHLEFRTPALYRLVRHPLYVGWFFAFWATPTMTVSHLVFALATTGYILIAIQLEERD
                     LIAHFGQTYRDYKKRVPMLVPFTRKRNVDDAFLRRLRG"
     gene            complement(3651..4043)
                     /locus_tag="VIV63_10725"
     CDS             complement(3651..4043)
                     /locus_tag="VIV63_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125117.1"
                     /translation="MKFTTVGPEGARNILREKQRRNRMAAKELRVQFPEFASIRLNFE
                     FTDETSQPPAPQSIVLHPPARAYFVYPCPYASCNGEFDLAANVADIAKQGQSESAGHT
                     TCKGERVRGRQGKTPCDLHIEYSIAVGK"
     gene            complement(4110..4403)
                     /locus_tag="VIV63_10730"
     CDS             complement(4110..4403)
                     /locus_tag="VIV63_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY6125118.1"
                     /translation="MTKLYVGNLPFSATEDSVRALFATHGNVDKVALINDRDTGQPRG
                     FGFVEMSSADAQKAMQSLNGQDFGGRPLKVNEAQDKPRGGGGGGGGGGGYGRR"
     gene            complement(4578..5264)
                     /locus_tag="VIV63_10735"
     CDS             complement(4578..5264)
                     /locus_tag="VIV63_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY6125119.1"
                     /translation="MFMRRAISTLAMTAILLAASNAAQAQGPDLQQRFEKKVQEYEAG
                     DKTSAPPTGAILFAGDSQFYRWKTIHEDLPGYTLINRGIDSFQFPDLLRYFDRLVLPY
                     KPRLIVLHVGGNDVHNGRTPAQVLADFQAFVARVRASMPGVRVLYSSITPGPGRWDEA
                     PQRRETNQAIRRYVESQADLGFVDLWEAMLTPAGQPREELWVADRVHPNHDGYLIRVK
                     LTQPLLGPPG"
     gene            5298..5693
                     /locus_tag="VIV63_10740"
     CDS             5298..5693
                     /locus_tag="VIV63_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125120.1"
                     /translation="MIGELESAFLRACPSFDGPLKSLREDHKLFGADLQTELTEILDR
                     LGFHLGSTAKQVPTPEMIQVLKVVERVLMDGSDQQKWAVTHFLMTSLQTSIKGAGLKA
                     DALQPHLGPATATWWNDLTKNPRAAEDWQ"
     gene            complement(5694..6809)
                     /locus_tag="VIV63_10745"
     CDS             complement(5694..6809)
                     /locus_tag="VIV63_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY6125121.1"
                     /translation="MKVPPFKLDAWLMAHEFATPPIRHNIASSTGPLWTLAELMALGG
                     EAGSAAALGDLTLSYAPPQGSKLLRERIAALHGVDPEHVLVMTGASEALVALTCLAAE
                     PGGSIVIPKPVYPAVPVLARAWGLKVREYELDRERGFAQTAEGVLAVVDSSTRLVFVN
                     TPHNPTGAVMLPVEQRKLAEALAARGIPLVVDEVYHPLYFGERSPSASTLPNTIVLGD
                     FAKALSIPGLRIGWLVDRDASRREALIDMRSYFTISGSPLTEAIGAHVLAHAPAIIAR
                     LTQVAQGNLALLTQFMEQHHKVLGWAPPGGGTTCFPWLRDGRDARPLCEALAKAGVLA
                     APGDCFDMPEHMRVGFGAMRSGYADALRIFSETLAKV"
     gene            6907..>7610
                     /locus_tag="VIV63_10750"
     CDS             6907..>7610
                     /locus_tag="VIV63_10750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6125122.1"
                     /translation="MDLKLAGKLALVSGSTAGIGFSIAETLAVEGARVIVNGRTPSGV
                     DAALAALRKSTNKEAIGFAGDLSDAAVAEALVRQHPGIEILVNNLGIFEPKAFEEIPD
                     ADWVRFFEVNVLSGARLARLVLPAMRRANWGRIIFISSESAVQIPAEMIHYGTTKTAQ
                     LAVSRGLAESLAGTGVTVDCVLPGPTRSRGVEEFVDTLAKSEGKSFAAFEKEFFEKVR
                     PTSLIKRFATPEEVASL"
BASE COUNT         1532 a         2435 c         2404 g         1239 t
ORIGIN      
        1 aagccggcac agaagctggc cgccgctcga gcggctgcgg ccgcgactgt cgctgcgccg
       61 gtcgaaggca agcccggcaa gggcaagaaa ggttccgcgg agctcatcaa ccttccggct
      121 cgccgcaacg tgctgccgga agagcgccag tcgcagctca agctgctgat cgcgcgcggc
      181 aaggaacagg gttacctcac ctactcccag gtgaacgacc acctgccgtc ggagatcgtc
      241 gacccggaac agatcgaaga gatcgtaaac acgatcaacg acatgggcat cccggtgtac
      301 gaaaaggcgc cggacaacga gagcattctc gcgcccgagc cttccgcgcc caccgacgaa
      361 gaagccatcg aagaagccgc tgccgcgctg gccgcgctcg atgccgagct cggccgcacc
      421 accgacccgg tgcgcatgta catgcgcgaa atgggcacgg tcgaactgct gacgcgcgaa
      481 ggcgaaatcc gcattgccaa gcgcatcgag gaaggcctcg acgccgtgcg cctcgcgctg
      541 tctaactatc cgtcgacgtc cgagtacctg ctgcgcgcgt acgaaccggt aaagaccggc
      601 ggcgcccgcc tggtcgacat catcgtgggt ttcatcgacc cgaacgcgcc cgacgtcatc
      661 gcgcagccgc agaaccccac caagatggat ctcgcggcgc ccgtgcctga agagaagccg
      721 gaaggtgagg agaccgagga aggcgacggc accggtgaag aagaggaagc cgtcgacacc
      781 ggcccggatc cggaagaggc cgcgcgccgc ttcgcggcgg tcggcaaata tctcgcgaat
      841 tcgctgacgc acatcgagaa gatgggcgcc aaggatccga agacgctgaa ggtccgcaag
      901 cgcctgtcgg acgagttcat ggagctcaag ctggcgccgc gcatgttcga ggcgctgatc
      961 ggcaacctgc gcaaccaggt caaccagatc cgccagctcg aaaaagaaat catgatcatt
     1021 gccgtgcgtg atgccggcat gccgcgcaag gaattcatcc tctcggtccc gaagaacgag
     1081 acttcgaacc gctggctgca gaagcaggtc aagggcggca agaagtggtc gaacgcgctc
     1141 ggccgcctgc aggacgagat ctcgcgccgc caggaaaagc tgagcacgct cgagaaggaa
     1201 taccacctca cgatcacgga catcaaagag atcaaccgtg aagtctcgat cggcgaagcg
     1261 aaagcccgcc gcgcgaagaa ggaaatggtc gaggcgaacc tgcgcctcgt gatctcgatc
     1321 gccaagaagt acacgaaccg cggcctgcag ttcctcgacc tcatccagga aggcaacatc
     1381 ggcctcatga aggccgtgga caagttcgaa taccgccgcg gttacaagtt ctccacgtac
     1441 gccacgtggt ggatccgcca ggccatcacg cgttcgatcg ccgaccaggc gcgcaccatc
     1501 cgcatcccgg tgcacatgat cgaaaccatc aacaagctga accgcatctc gcggcagatg
     1561 ctgcaggaga tgggccgcga gccgacgccg gaagaactgg ccgtgcgcat ggaaatgccc
     1621 gaggacaagg ttcgcaaggt gctcaagatc gccaaggaac ccatctcgat ggaaacgccc
     1681 atcggtgacg acgaggactc acacctcggc gacttcatcg aggacacgtc ggtggcgtcg
     1741 cccatcgacc aggcgaccat ggagtccctg cgcgaaacca ctcactcggt acttgcgcaa
     1801 ctgaccccgc gtgaagccaa ggtgcttcgt atgcggttcg gtatcgacat gaacaccgac
     1861 cacacgctgg aagaagtggg caagcagttc gacgtgacgc gcgagcgtat tcgccagatc
     1921 gaggcgaagg cgctgcgcaa gctgcgccat ccgtcgaggt cagagcagct gcgaagcttc
     1981 ctcatcgaag actgagaaaa aagggccgcc gcaaggcggc cttttccatt ccatggctga
     2041 aaaaccggcg gattggcagc ggcagtttta ctccaggccc ggcggtcgcc cattcctgtt
     2101 ctacgtggct ttcggcagtc ccgatggccc actcaaaatc tcagcgtctc agtatcgagt
     2161 cggaggcatg cccgatggac tcgagatgtc gggttatggc ccggaccggc atcccggtga
     2221 ggcgaacaaa ttccgggagg gttatctctg gaacgaactg cagcgcgagg atgcggcgct
     2281 ggccgcggca atcgccgcgc aggggcactg caccgtgctt cgcgccgacc tcgaagacca
     2341 gcccacgctc aactatcttc gcgatacgat cggcatcgcg acctatctac tggatcacgg
     2401 ctgtgtcgcc atctacgacc cgcaaatgat ctgctactgg tcgccttcgc ggtggcgctc
     2461 gaaggtcttc gatccggcgg ccgcggtgcc ccgcaatcac gtgatgattc tgtacgacgg
     2521 tgacggtgaa ggcaccgagt ggtttcacac gcgcggcatg cgcaaattcg ggcgcccgga
     2581 tctgagcgtg ccccgcacac cggcgaaaca tcgcgacgcg gtcatcgatc tcatcaaccg
     2641 tttcatcgag taccaggcct tcggaggcgt catcgaagaa ggccgggaga tcaaggtgaa
     2701 cgatctcccg gctggcatgc gctgttccca ccacgggaac ctcgacgacc ccgacttcaa
     2761 caacgtgcac gttcgaatcg actggtcctg atcagccccg gagccggcgc aggaacgcgt
     2821 cgtcgacatt ccgcttgcgc gtgaacggca cgagcatcgg cacgcgcttc ttgtaatccc
     2881 gatacgtctg cccgaaatgc gcgatgagat cgcgctcctc gagctggatg gcaatcagga
     2941 tgtagcccgt ggtcgcgagg gcgaacacca ggtgcgacac ggtcatcgtg ggcgtcgccc
     3001 agaacgcgaa gaaccagccg acgtacagcg ggtgtcgaac caggcgatac aacgccggcg
     3061 tgcggaattc gaggtgagtg tacggacggc ccatcaactg cagccagacc tggcgaaggc
     3121 cgaacaggtc gaagtggttg atgaggaatg tcgacaccag cacgagcagc cagccgaacg
     3181 cgaagcagcc ccagatgaca ccgcgcgcgg tggcatcggt gacggtccag acaacgccgc
     3241 ccaacggctg ccacccatag aacatcgcga tcagcgcgac gctcgacagc aggacgtacg
     3301 tgctgcgttc agcggattcc ggcaccagct tcgtgaacca gcgtttgaac gcgggacgcg
     3361 ccatgacgct gtgctggatc gcgaacaggc ccaggagccc gaggttcacc agcaatgcgg
     3421 tagtgaggtc ggacgcgggc gcgccgtcga gcgttcgcgg caccgcgaag ttgccgacga
     3481 agccgatggc atagaggaac gtgagaaaga acacggcgta actgacgacg ccgtacagaa
     3541 agatcgacag acgtttgaac atgattcact cccggatgat cgaggccggc acgttaggcc
     3601 ccgatcgtgg gagcgggcgt ggtaacgccc gtggaatcgt cagcgtggag ctacttgccc
     3661 accgcgatgg aatattcgat gtgcaggtcg cagggagtct tgccctgtcg gccgcgaacg
     3721 cgctcgcctt tgcaggtggt atgcccggcg gattcgctct gaccctgctt ggcaatgtcg
     3781 gccacattgg cggcgagatc gaactcaccg ttgcagctgg catacggaca cggatagacg
     3841 aagtaagcac gcgccggcgg gtgcagcacg atggactgcg gagccggggg ctgcgaagtc
     3901 tcgtcggtga actcgaagtt gaggcggatc gacgcgaatt cggggaactg cacgcgcagt
     3961 tccttggcag ccatgcgatt acgccgctgc ttctcgcgaa ggatgtttcg cgccccttcg
     4021 gggcctaccg tggtgaattt catttggttt gaatgccgcg accgacgcgg gtgggctttt
     4081 gccccacccg catcgtcgca gccgatgtct tatcgacgac cgtagccgcc gccgccacca
     4141 ccaccgccgc caccgccgcg gggtttgtcc tgcgcttcgt tgactttgag cgggcgaccg
     4201 ccgaagtcct ggccgttcag cgactgcatg gccttctgcg cgtcagcgct cgacatttcc
     4261 acgaagccga agccgcgcgg ctggcccgtg tcacggtcgt tgatcagcgc gactttgtcc
     4321 acgtttccgt gggtcgcgaa gagggcacgc accgaatctt cggtggcgct gaagggaaga
     4381 tttccaacgt aaagtttggt caagagaata ctcactaagt aaatgtagat aggcagggga
     4441 accctgcgag agcgactctg gccaaacttc gcgtcaggaa gacgcgctag cccagggacg
     4501 gacaagacga ggaatcacaa gccgggcggc acagtacagg atttgtcgcc tctttgcccg
     4561 ggttcttgca gggcccgtca gccagggggt cccagcagcg gctgagtcag cttcacccgg
     4621 ataaggtaac cgtcgtggtt ggggtggacg cggtcggcaa cccacaactc ttcacgcggc
     4681 tgcccggctg gggtgagcat ggcctcccag aggtcgacaa agccgagatc ggcctgactt
     4741 tccacgtatc ggcggatcgc ctggttggtt tcccgccttt gcggcgcctc gtcccaccgc
     4801 ccgggcccag gagtaatgct cgaatagagg acgcggaccc cgggcatcga ggcccgcacc
     4861 cgggccacga aagcctggaa atcggcgagg acctgcgccg gcgtccggcc gttgtggacg
     4921 tcattgcccc ccacgtgcag cacgatgagc ctgggtttgt acggcaacac gagcctgtcg
     4981 aagtaacgca gcagatccgg gaactggaat gagtcgatgc cgcggttgat cagcgtgtag
     5041 cccggcaggt cctcgtgaat cgtcttccac cgatagaact gcgagtcgcc ggcgaacagg
     5101 atcgcccccg ttggcggagc gctggtcttg tcacctgctt cgtattcctg aaccttcttc
     5161 tcaaaacgct gctgcaagtc cggaccctgc gcttgcgccg cattggacgc tgccagcagg
     5221 atagccgtca tggctaatgt cgatatcgcg cggcgcatga acattccgca ttcccccttc
     5281 gaagctgcga acgttacatg atcggcgagc tcgaatccgc gttcctgcgc gcctgccctt
     5341 cgttcgatgg accgctgaag agcctgcgcg aagatcacaa actgttcggc gcggatctgc
     5401 agaccgagct cacggagatc ctcgaccggc tcggctttca tttggggagc accgcaaagc
     5461 aggtgccgac accggagatg atccaggtgc tcaaggtggt cgaacgcgtg ctcatggacg
     5521 gcagcgacca gcaaaagtgg gccgtgaccc attttctgat gacgtcactg cagacctcga
     5581 tcaagggcgc cggtctcaag gccgatgcgc tgcagccaca tctcggtccc gccaccgcaa
     5641 cctggtggaa cgacctgacc aagaatcccc gcgccgccga ggactggcag tagctagacc
     5701 ttggccagcg tctcgctgaa gatgcgcagc gcatccgcgt agccgctgcg catcgcgccg
     5761 aatcccacgc gcatgtgctc cggcatgtcg aagcaatcgc ccggcgccgc cagcactccc
     5821 gctttcgcca atgcctcgca cagcgggcgc gcgtcacgcc catcccgcaa ccagggaaaa
     5881 cacgtggtgc cgccacccgg aggcgcccat cccagtacct tgtgatgttg ctccatgaac
     5941 tgcgtgagca gcgccaggtt gccctgcgcc acctgcgtga gccgggcaat gatcgccggc
     6001 gcgtgcgcga gcacgtgcgc gccgatggcc tcggtcagcg gcgagccgga aatcgtgaag
     6061 tagctgcgca tgtcgatcag cgcttcgcgc cggctcgcat cgcgatcgac gagccatccg
     6121 attcgcagtc ccggaatgga tagcgccttg gcgaagtcgc cgagcacgat ggtgttgggc
     6181 agcgtgctgg cactcggact gcgctcgccg aaatacaacg ggtggtacac ctcgtcgacg
     6241 acgagcggga tgccgcgcgc cgccagcgct tcggccagct tgcgctgctc caccggcaac
     6301 atcaccgcgc ccgtggggtt gtgaggcgta ttcacgaaaa ccaggcgggt gctggaatcc
     6361 accaccgcca gcacgccttc cgcggtctgc gcgaaccccc gctcgcgatc cagttcgtac
     6421 tcgcgcacct tcaggcccca ggcacgcgcc agcacgggca ccgcggggta cacgggcttc
     6481 gggatcacga tggagccacc gggctcagcg gccaggcagg tcagcgccac gagcgcctca
     6541 gaggcgcccg tcatcacgag cacatgttcc ggatcgacgc cgtgcaatgc agcgatacgt
     6601 tcgcgcagta gtttgctgcc ctgtggtggt gcgtaggaca acgtgaggtc gccgagcgcc
     6661 gccgcgcttc ccgcttcacc gccgagggcc atgagttccg ccagggtcca caacggcccg
     6721 gtgctcgagg caatgttgtg gcgaatgggc ggcgtcgcga actcgtgcgc catgagccag
     6781 gcgtcgagct tgaatggtgg gactttcatg gcgttatgat cctgcgcatg ccgcgcgatc
     6841 acaatgccgc ctcgaaggcc ggagcgcgca ggcgtatctt gactccatcg aattcgaagg
     6901 aaacacatgg acctgaaact cgcgggcaaa ctcgcgctcg tctccggcag caccgccggc
     6961 atcggcttct ccatcgccga aacgctggcg gtggaaggcg ctcgtgtcat cgtcaatgga
     7021 cgcacaccgt cgggcgtcga tgcggcgctc gccgccctgc gcaagtcgac taacaaagag
     7081 gccatcgggt tcgccggcga tctgtccgac gctgcggtgg ccgaggccct ggttcgccag
     7141 cacccgggca tcgagatcct ggtcaacaat ctcggcatct tcgaacccaa ggccttcgag
     7201 gaaattcccg atgcggactg ggtgcgcttc ttcgaagtga acgtgctctc gggcgctcgg
     7261 ctggcgcggc tcgtactgcc ggccatgcgc cgcgcgaact ggggtcgcat catcttcatc
     7321 tccagcgaaa gcgccgtgca gattcctgcc gagatgattc actacggcac gacgaagacc
     7381 gcgcagctcg ctgtctctcg cggcctcgca gaatcgctcg cgggaacggg cgtcacggtc
     7441 gactgcgtgc tgccgggtcc gacgcggtcg cgcggcgtgg aagagttcgt cgacacgctc
     7501 gcgaagtccg agggcaagag cttcgcggcc ttcgagaaag agttcttcga gaaggtgcga
     7561 ccgacgtcgc tgatcaagcg cttcgccacg cccgaagaag tggcctcgct
//
LOCUS       DATDEU010000450        10688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1980746, whole genome shotgun sequence.
ACCESSION   DATDEU010000450 DATDEU010000000
VERSION     DATDEU010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980746"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VIV63_10755"
     CDS             complement(<1..727)
                     /locus_tag="VIV63_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125123.1"
                     /translation="MANKIPAIVLGGTGYVSGELLRLILGHPDFTLAGVMSDSSPGEP
                     AGKSFPHLAGVLGTTAFKSQVEIAALIAKEPTSAVFCAAPHGAAAALIDSLLKAAEAA
                     GTKPRFVDISADFRFATAAGYEAVYKHPHGAPHRLAQFTCAVPEHLKKVSTQHVGHPG
                     CFSTAILLASVPLLSSGIAEPTLFVSGVTGSTGSGRKPVEGTHHPTRHSDLYSYGALG
                     HRHTPEITALAKAASGVEAEFAFV"
     gene            complement(733..1818)
                     /locus_tag="VIV63_10760"
     CDS             complement(733..1818)
                     /locus_tag="VIV63_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125124.1"
                     /translation="MRTVVVDKIASVTQACGLGNEVRVATDNIPAEEGVVVVVEILSN
                     KSTYNTLELTSGRMAKVGKGDIVAGALGHRKALFGYSGHVPESVKPGDIIQMLNIGGV
                     LGIVDSVNPDKGKPFDCRVIGGVLHFPYLGERIGVPARVGYRQLDYAAKLATNGVPVV
                     ALAGTCMEAGKTAAAAAIISRMRHRGLVVDAFKATGVSLRRDIMAFEDAGARNTLIFT
                     DFGIVSTTRKVGPALTRTMLTELSEKKPDVIVFELGDGLLGTYGVDSILEQEDIRKAL
                     TGVVLSANDPVAAYGGVDILRKRFNIEPVAVTGPATDNDVGVSIIRDQLKVPAFNALA
                     AGAALGDSIIESVGLTQARPLDRVANE"
     gene            complement(1815..3053)
                     /gene="argG"
                     /locus_tag="VIV63_10765"
     CDS             complement(1815..3053)
                     /gene="argG"
                     /locus_tag="VIV63_10765"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY6125125.1"
                     /translation="MSAQVSNNDKPIVLAYSGGLDTSFLVPWLKENYNRPIITVTVDT
                     GGIDAAGAKTLQERSKAYGAIEHHQIDARADYFEQVLRFLIMGNVRRGQLYPLCVGAE
                     RVMQAQTIALMARKLGTNSIAHGCTAAGNDQVRFEVAMRTLAPELEVIAPVRDKAFKR
                     QEELDYLKARNLPIPPYGAAYSINRGLWGVTIGGKETLTSEGSIPDSAWVLSKDAFSN
                     PRPPAKHTIHFEKGRPTGLDGKTMSAVDVVEQLETLGAPYGIGRGIHLGDTIIGTKGR
                     VAFEAPAAETLLTAHRELEKLVLTPRQQRIKDSLAGPYGDWVHEGQLLDPVCRDIEAL
                     LLSSQERVTGKVHVLFRPGSLFIEGVESQYSLMAVSKGVYGESHGEWTPQDALGFSKI
                     VSLTGMFHARAGEKKMGTAK"
     gene            complement(3060..3518)
                     /locus_tag="VIV63_10770"
     CDS             complement(3060..3518)
                     /locus_tag="VIV63_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125126.1"
                     /translation="MLTDHHREARRTAIVRLLRHSPVRRQQELVRLLKREGHAVTQSS
                     ISRDLRELGVLKASDGYVLPDKAELSSRTQDKFSAVASFVREVRTAGPSITVVKTTIG
                     SAGSVAAAIDKAGWNEVAGTVSGDDTIFIATADARAQARVLEQLREAFGV"
     gene            complement(3643..3948)
                     /locus_tag="VIV63_10775"
     CDS             complement(3643..3948)
                     /locus_tag="VIV63_10775"
                     /inference="COORDINATES: protein motif:HMM:NF009508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HEY6125127.1"
                     /translation="MHFLLLYTYVPDVLERRPQFRGAHVKHAREYVARGELVLGGAFA
                     DPVDGAVLFFSVPSKDLVETFAKTDPYVTGGLVTEWKVREWTTVIGKDAAHPLPPGL"
     gene            3996..4595
                     /locus_tag="VIV63_10780"
     CDS             3996..4595
                     /locus_tag="VIV63_10780"
                     /inference="COORDINATES: protein motif:HMM:NF007458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HEY6125128.1"
                     /translation="MHRIIALTLLVLAFIAPRVQAASPLRDGDIIFHTSRSAQSAAIQ
                     RATHSPYSHMGVVLHRDGKPFVFEAIATVRYTPLANWTARGDGGRYEVRRLKRKLTPA
                     EIGKLHASAKAYEGKPYDLYFEWSNARIYCSELVWKMYRDALGIEVGTLQRLREFDLT
                     DPAVRAKMRERYGTKVPLDEQVISPAAMFESSILETVPR"
     gene            4608..5510
                     /locus_tag="VIV63_10785"
     CDS             4608..5510
                     /locus_tag="VIV63_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125129.1"
                     /translation="MRSIVSLTSGVAALLLLAGLPAVTHAAEKPKRVKYVAPEGFAGR
                     AWGELRTSAAFDRLPKEPMGVGAAWMSPREKEVSFTCVPISAPGPTMNGAVEGCDFQA
                     TLLRLQRKFEGGGFYVLSEYSIDGQGFRYGDENDGLVLHPVIYQFCANWDETKREVPP
                     KFDDINKFCGVRLMFQSDTREQLRKQPIEYVTNYDRMLEKLLAKFGKPDNFTRRGKVV
                     IETLEGDSSDQADRKFSIWRWCPARDRGFHTICKASVVLSLDPATGVGTILYSTPLLW
                     EFAYARENNGYKGDKLFKMLHARK"
     gene            complement(5519..6163)
                     /gene="alkB"
                     /locus_tag="VIV63_10790"
     CDS             complement(5519..6163)
                     /gene="alkB"
                     /locus_tag="VIV63_10790"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: protein motif:HMM:NF011930.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEY6125130.1"
                     /translation="MNTLELPFADASHERLRLGDGITLLRGFADTAQLMPLIHSIASV
                     APFRHLVTPGGQTMSVAMTNCGPVGWVSDRSGYRYSSRDPLTDQDWPALPREFEQLAL
                     TAASASGFPDFVPDACLINRYVAGARLTAHRDADEQNFAQPIVSVSLGLPASFAFYGL
                     TRGGKGQTVALTDGDVLVWGGPSRLVYHAVRPIKPGKHPLTGEARYNLTFRHAR"
     gene            complement(6160..9912)
                     /locus_tag="VIV63_10795"
     CDS             complement(6160..9912)
                     /locus_tag="VIV63_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY6125131.1"
                     /translation="MIKRGLKIAALVVALLLLAILAFLAWVLYTEAGLRFAVARLPER
                     MGKVTLHIENIHGTIAGGFGADLADIDHERSHVRVVNGSARVNFWPILVGRIAVREAR
                     ADVVQIDVRRRLTPPPNNTARFLPRLLSISAEKASARTLVIVAPSGKRVEFDNVTGSG
                     TVGHKTIRVFDSNVVYGVLQARAIGELRAADIMELSGEATTRMIIEGQPTWRADSSFE
                     GTLDKLPLTLKLFEPFRADMRGELLSLSSNFHWTGKADVHNFDLQAFGGGSALGIIKG
                     PLDVGGEMNEFFARGPLMVPGLGSGPFDILFEGNYADRVVNATHYEVTHKATGSHVEG
                     QGTIEPAENGPQLLLYGDWRGLRWPLVARFTEETPQLFSSPEGKYRLEGLWPYAITAS
                     GDLFIPQLDPMTVAVRGALHKDHLQMDDLVVGAFEGKATLAGEARWSPEESWSLEGKV
                     KGINPARLRPGFNGALDFNMKASGAPFGGDGAIDFAFSDLVGKLRGNTATGSGRVKLQ
                     GDNYTFNALRFRAGNTRLAIDGDIGSSRALNLDFSLDADNLALLKEGASGELHATGRI
                     GGTSDAPSIKLTARGNDIESGTMSVDKLSANIDLDWRGQRISHADVAVSGLNVGERSL
                     TQFNATLDGTTAEHTIRADALAGITSLHLSGKGGFADGVWNGTIGDLFIDDTANINLQ
                     LDTPVKVRASAKDVKIDALCMHGKVARLCGEGAWNEAGWTARGRANNLPISTLTAGLT
                     PNVEYQGTLNATASASASGGAPIVGEARVDLSDAAIRHKLASGRTDVITFGSGFVTLK
                     ADPAQINGELRLDAEQRGLISGRFRADRVTADIMDSPMRGQLQMATGELGFLTLYFPA
                     IDRASGRFDTNITLEGTLGKPSASGVIKLSGAELDLYQLNLALRAVEMEARIVSNRLE
                     FSSTAKAGAGTLSSAGEIEWRDNLPYGEVRVDGENLRVVDVPEARIDASPNLDFRIAG
                     REIMVKGEVKVPLARIQPADLTNAVLASADERLVGPTEEVEKDPFRVTSEITMTLGEK
                     VTIDTYGLVGHITGSITERTLPGEPTRASGELQVKDGTYTALARKLDIERGRLIFSGG
                     LLADPAVDIRAIKEFPDVKAGVNVRGSLREPRLTFFSEPTIPQAQIVSLLLAGGSLQS
                     VQDQNRAGTPAASQEVAGQAAAILAAQLGGKIGIPDISVESTLSNETSLVLGKYLSPR
                     LYVSYGVSLTESINTIKMRYTINDRWTIKTESGQEQAADLVYTIEK"
     gene            complement(9909..>10688)
                     /locus_tag="VIV63_10800"
     CDS             complement(9909..>10688)
                     /locus_tag="VIV63_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY6125132.1"
                     /translation="DRKVNFIPSYTHVRGPWFGHFWQRVTYVELLHAESEFIASNRTD
                     TQSLLIPGISFSLVPRNYLGEALFSRALYAELRGSHDALGSDSNFLQIRMQGERVFDL
                     APKWHVLVRGDIGATAVDSTSGLAPSQRFFAGGDRSVRGFGFNELSPVAQATDANNQP
                     LFDEDNNPVYEKVGGKHLFAGSLEVVRDVGSKFAVAVFADAGNAFDSFDDPLMYSVGI
                     GVRLRLPVVSVGLDVAQALTTPAGSTERPGPRLHLNFSPKL"
BASE COUNT         1904 a         3287 c         3562 g         1935 t
ORIGIN      
        1 gcacgaacgc gaactcggct tcgacaccgg aagccgcctt ggcgagcgcg gtgatctcgg
       61 gcgtatgccg gtgacccagc gcgccgtagg aatagagatc gctgtgacgc gtcgggtggt
      121 gtgtgccttc gacaggtttg cgacccgagc ctgtgctgcc ggtgacgccg ctgacgaaca
      181 gcgtgggctc ggcaatgccc gaagacagca aaggcacgct cgccagcaga atggccgtgg
      241 agaagcagcc cgggtgcccc acatgttggg tggacacctt cttgaggtgc tcgggaacgg
      301 cgcaggtgaa ttgcgcgagg cgatgcggcg cgccatgcgg atgtttgtac acggcttcat
      361 aacccgcggc cgtggcgaag cggaagtccg cggaaatgtc gacgaaacgc ggcttggtgc
      421 ccgcggcttc ggcggccttg agcagcgagt cgatgagcgc ggcggcagcg ccatgcggcg
      481 cggcgcagaa caccgcggac gtgggttctt tcgcaatcag ggcggcgatc tccacctgcg
      541 acttgaatgc ggtggtgccg agcacgcccg cgagatgcgg aaaggatttg cccgcgggtt
      601 caccgggcga actgtcggac atcacgcccg cgagcgtgaa gtccgggtgg ccgaggatga
      661 ggcgcagcag ttcgccggac acatagccgg tgccgccgag aacgatggca gggatcttgt
      721 tggccatgac gattattcgt tcgcgacgcg atcgagcgga cgcgcctgcg tgaggcccac
      781 ggactcgatg atcgagtcgc cgagcgccgc gccagcggcc aacgcgttga acgccggcac
      841 cttgagctga tcgcggatga tggacacacc cacgtcgttg tccgtcgccg ggcccgtgac
      901 cgcgaccggc tcgatgttga agcgcttgcg caggatgtcg acgccgccat aggccgcgac
      961 cggatcgttg gccgagagca cgacgccggt gagcgccttg cggatgtctt cctgctcgag
     1021 gattgaatca acgccgtagg tgcctaacaa gccgtcaccc agctcgaaca cgatgacgtc
     1081 gggcttcttc tcggagagct cggtcaacat ggtgcgcgtg agcgcggggc ccaccttgcg
     1141 cgtggtcgag acgatgccga agtccgtgaa gatgagcgta ttgcgcgcgc cggcgtcttc
     1201 gaacgccatg atgtcgcggc gcagggaaac gcccgtggcc ttgaacgcgt cgaccacgag
     1261 gccgcgatga cgcatgcgcg aaatgatggc ggctgcggcg gccgtcttgc cggcttccat
     1321 gcaggtgccg gcgagcgcca cgaccggaac gccattggtg gcgagtttgg cggcgtagtc
     1381 gagctggcga taaccgacgc gcgccggcac gccgatgcgt tcgcccaagt aggggaaatg
     1441 cagcacgccg ccgatgacgc ggcaatcgaa cggtttgccc ttgtcggggt tcaccgagtc
     1501 gacgatgccc agcacaccac cgatgttcag catctggatg atgtcgccgg gcttcacgct
     1561 ctcgggcacg tggcccgagt aaccgaacag cgccttgcga tggccgagcg cgcccgcaac
     1621 gatgtcgcct ttgcccactt tggccatgcg gccgctggtg agctcgagcg tgttgtaggt
     1681 ggacttgttg ctgaggattt ccacgaccac gaccacgcct tcctcggcgg gaatgttgtc
     1741 ggtggcgacg cgcacttcat tgccgaggcc gcaggcctgg gttacggacg caatcttgtc
     1801 gactactaca gttctcattt ggcggtaccc attttctttt ctccggcacg ggcatggaac
     1861 atgcccgtga gcgaaacaat ctttgaaaaa cccagtgcat cctgcggcgt ccattcgccg
     1921 tgggactcgc catacacgcc cttggacacg gccatgagcg aatactgcga ttcgacgcct
     1981 tcgatgaaca aactaccggg acggaacaac acgtgcacct tgccggtgac gcgctcctgg
     2041 gacgacagca gcagcgcttc gatgtcgcgg cacacggggt ccagcaactg gccttcgtgc
     2101 acccagtcac cataagggcc ggcgagtgaa tccttgatgc gctgctggcg cggcgtgagc
     2161 acgagttttt cgagctcgcg atgcgcggtg agcagcgttt cagcggccgg cgcttcgaac
     2221 gcgacgcggc ccttggtgcc gatgatggtg tcgccgaggt ggatgccgcg gccgatgccg
     2281 tacggcgcgc ccagtgtttc gagctgctcg acgacgtcga cggccgacat ggtcttgccg
     2341 tcgaggccgg tcgggcgacc cttctcgaaa tgaatggtgt gcttcgcggg cgggcgagga
     2401 ttgctgaacg cgtccttcga caacacccag gcgctgtcgg gaatgctgcc ttcggaggtc
     2461 agcgtttcct tgccaccgat ggtcacgccc cacaggccgc gattgatgga ataggccgcg
     2521 ccgtacggcg gaatcggcag attgcgcgcc ttcagatagt cgagctcttc ctggcgcttg
     2581 aacgccttgt ctcgcaccgg cgcgatcact tcgagctcgg gcgccagcgt gcgcatggcg
     2641 acttcgaaac gcacctggtc gttacccgcg gcggtgcagc cgtgcgcgat ggaattcgtg
     2701 ccgagcttgc gcgccatgag agcgatggtc tgcgcctgca tgacacgctc cgcgcccacg
     2761 cacagtggat agagctggcc gcggcgcacg ttgcccatga tgagaaaacg cagcacctgc
     2821 tcgaagtaat ccgcgcgcgc gtcgatctgg tgatgctcga tggcgccata cgcttttgaa
     2881 cgttcctgca gggtcttcgc gcccgcggcg tcgatgccgc cggtatccac ggtcaccgtg
     2941 atgatggggc ggttgtagtt ttccttgagc cacggcacca ggaacgaggt gtcgagaccg
     3001 ccggaatagg caagaacaat gggcttgtcg ttattggaaa cctgggcact catttttatt
     3061 cagactccaa aagcttcacg caactgctcg agcacgcgcg cctgcgcgcg ggcatcggcc
     3121 gtggcgataa aaattgtgtc gtcgccggac acggtgcccg cgacttcatt ccagcccgcc
     3181 ttgtcgatgg cggcggccac actgccggcc gagccgatgg tggtcttcac cacggtgatc
     3241 gacggcccgg cggtgcgcac ttcgcgcacg aacgaggcca cggcggaaaa cttgtcctgc
     3301 gtgcgcgaag aaagctccgc cttgtccggc agcacgtagc cgtcgcttgc cttgagcacg
     3361 ccgagttcgc gcaggtcgcg gctgatcgaa gactgcgtga ccgcgtggcc ttcgcgcttc
     3421 aacaggcgca cgagctcctg ctggcggcgc accggggagt ggcgcagcag gcgaacgatg
     3481 gcggtacgac gcgcctcgcg gtgatggtcg gtgagcatgg aactgaatcc acccatgaat
     3541 aaagagcgcg catggtgcat aaaaatgcac aaggcgtcaa cgattctttt accccaccag
     3601 gacgtgggac tcgttaacta cagagccggg attgcgcgtg cgttacaggc cgggcggaag
     3661 ggggtgcgcg gcatccttgc cgatgaccgt ggtccattcc cgaaccttcc attcggtgac
     3721 caggccaccg gtcacgtagg ggtcggtctt ggcgaaggtc tcgaccagat ctttggacgg
     3781 cacggagaag aacaggacgg caccatcgac cgggtcggcg aatgcgcctc ccaggacgag
     3841 ttcgccgcgg gcgacgtatt cgcgcgcgtg tttgacgtga gcgcctcgaa actgcgggcg
     3901 gcgctcgagg acgtccggga cgtacgtata taagagtagg aagtgcatgg gtgcgaatca
     3961 tagtggagcg gaatgctcac gcgtaccatt ccgcgatgca ccgaatcatc gcgctgacct
     4021 tgctggtcct ggccttcatc gcgcctcgcg tgcaggcggc ttcgccgctg cgcgacggcg
     4081 acatcatctt ccacacctcc cggtcggcgc agagcgcggc catccagcgg gcgacgcatt
     4141 cgccgtacag ccacatgggc gtggtgctgc atcgcgacgg caagccattc gtgttcgagg
     4201 cgatcgccac cgtgcgctac acgccattgg cgaactggac ggcgcgtggc gatggcggac
     4261 gctacgaagt cagacgcctc aagcgaaagc tgacgcccgc cgaaatcggg aaactccacg
     4321 cgagcgcgaa ggcgtacgag ggcaaaccct atgacctgta cttcgaatgg tccaatgcgc
     4381 gcatctactg ctccgagctg gtgtggaaga tgtatcgcga cgcgctcgga atcgaagtgg
     4441 gcacactgca gaggctgcgc gagttcgacc tcacggatcc ggcggtgagg gcgaagatgc
     4501 gtgagcgata tggcacaaag gtgccgctgg acgaacaggt gatttctccg gcggcgatgt
     4561 tcgaatcgtc gattctggaa accgtgccga ggtaatatca gcgccccatg agatcaatag
     4621 ttagcctgac ttccggcgtg gccgccctcc tgctgctcgc cggtctcccg gcggtgacac
     4681 acgccgccga gaagcccaag cgcgtcaaat acgtcgcgcc cgaaggcttt gcgggccgtg
     4741 cctggggcga gttgcgcacc agcgcggcct tcgatcgcct gccgaaagag cccatgggcg
     4801 tgggtgcggc gtggatgagt ccgcgcgaaa aagaggtcag cttcacctgc gttcccatct
     4861 ccgctcccgg ccccaccatg aacggcgcgg tcgaaggctg cgatttccag gcgacgttgc
     4921 tgcgcctgca gcggaaattc gaaggcggcg gcttctacgt gttgtccgaa tactccatcg
     4981 acggccaggg ttttcgttat ggagacgaaa acgatggcct cgtgctgcac ccggtcatct
     5041 accagttctg tgcgaactgg gatgaaacca agcgcgaagt gccacccaag ttcgacgaca
     5101 tcaacaagtt ctgcggcgtg cgcctgatgt tccagagcga cacgcgcgag cagctgcgca
     5161 aacagccgat cgaatacgtg accaactacg accgaatgct ggagaaactg ctggcgaagt
     5221 tcggcaagcc cgacaacttc acccggcgcg gcaaggtggt catcgaaacc ctcgaaggcg
     5281 attcgtccga ccaggccgat cgcaagttca gcatctggcg ctggtgcccg gcgcgcgatc
     5341 gcggcttcca taccatctgc aaggcgagtg tggtgttgtc actcgatccc gcaacgggag
     5401 tcggcaccat tctgtattcg accccgctgc tgtgggagtt cgcgtacgcg cgtgagaaca
     5461 atggctacaa gggcgacaag ctcttcaaga tgctgcacgc gcgcaagtag tcaggccttc
     5521 agcgtgcgtg ccggaacgtg aggttgtaac gcgcctcgcc ggtcagagga tgtttcccgg
     5581 gcttgatggg ccgcactgcg tggtaaacca gccgcgacgg gccgccccac accagcacgt
     5641 cgccgtcggt aagcgccacg gtctgcccct tgccaccgcg cgtcagacca taaaacgcga
     5701 agctcgccgg cagtcccagc gacaccgaaa cgatgggctg cgcaaaattc tgttcgtccg
     5761 cgtcgcgatg ggcggtgagc cgcgcgcctg cgacatagcg attgatgagg caggcgtcgg
     5821 gaacgaagtc cggaaagccg gacgcggatg ccgcggtgag ggcgagttgc tcgaactcgc
     5881 gtggcagcgc cggccaatcc tggtcggtca gcggatcgcg tgagctgtag cgataaccac
     5941 tgcgatcgct gacccagccg accggaccgc aattggtcat ggccaccgac atcgtctgcc
     6001 cgcccggagt gaccaggtgg cgaaagggcg cgacgctggc aatcgagtgg atgagcggca
     6061 tgagttgcgc ggtgtcggca aagccgcgca gcagcgtgat tccgtcaccg agccgcagac
     6121 gttcgtggct cgcatccgca aatggcaatt ccagcgtgtt cacttttcga tggtgtagac
     6181 gaggtcggcc gcctgttcct ggccggactc ggtcttgatg gtccagcgat cgttgatcgt
     6241 gtaacgcatc ttgatggtgt tgatggactc ggtgagcgac acgccataac tcacgtagag
     6301 ccgcggcgac agatactttc cgagcaccag cgaagtctcg ttcgacagcg tcgactcgac
     6361 gctgatgtcg gggatgccga tctttccgcc caactgcgcg gcgagaatcg ccgccgcttg
     6421 cccggccact tcctgcgatg ccgccggcgt gccggcgcgg ttctggtcct gcaccgactg
     6481 cagcgaccca ccggcaagca gcagcgagac gatttgcgcc tgcggaatcg tcggctccga
     6541 gaagaacgtg agccgcggtt ctcgcagact tccacgcacg ttcactcccg ccttcacgtc
     6601 gggaaattcc ttgatggcgc ggatatccac ggccggatcg gctaacaacc cgccgctgaa
     6661 gatcaggcgg ccgcgctcga tatcgagttt gcgcgccagc gccgtgtacg tgccgtcctt
     6721 cacctgcaat tcaccggaag cgcgcgtggg ctctcccggc aacgtgcgtt ccgtgatgct
     6781 tccggtgatg tggcccacga ggccgtaggt atcgatggtc accttttcac cgagcgtcat
     6841 cgtgatttcg ctggtgacgc ggaacgggtc cttctcgact tcctcggtag gtcccaccag
     6901 tctttcgtcg gcggacgcca gcacggcgtt ggtgagatcg gcgggctgaa tgcgggccag
     6961 aggaaccttg acctcacctt tcaccattat ttcgcggccg gcgatccgga aatcgaggtt
     7021 gggggaggcg tcgatgcgcg cttcgggtac gtcgacgacg cgaaggtttt caccatcgac
     7081 gcggacttcg ccgtagggca ggttgtcgcg ccattcgatt tcgccagcac tcgagagcgt
     7141 gccggcgccg gccttggcgg tggagctgaa ctcgagccgg ttggagacga tgcgtgcttc
     7201 catctcgacc gcgcgcaatg cgagattgag ctggtagaga tcgagttcgg cgcccgatag
     7261 tttgatgacg ccactcgcgc tcggcttgcc gagcgtgccc tccagcgtga tgtttgtgtc
     7321 gaaacgcccg ctggcgcgat cgatggcggg aaagtagagc gtcaggaacc cgagttcgcc
     7381 ggtggccatc tgcagctggc cgcgcatcgg cgaatccatg atgtcggcgg tgacgcggtc
     7441 ggcgcggaat cgtccggaga tcaggccgcg ctgctcggcg tcgagccgca actcgccgtt
     7501 gatctgcgcg ggatcggcct tcagcgtgac gaaaccggag ccgaatgtaa tgacgtcggt
     7561 gcggccactc gccagcttgt gccggatggc ggcgtcggaa agatcgacgc gcgcttcgcc
     7621 gacgatgggc gcgccgccgg atgcgctcgc actggcggtg gcattgagcg tgccctgata
     7681 ttcgacattg ggagtgagcc cggccgtcag cgtgctgatc ggcaggttgt tagcccggcc
     7741 acgcgcggtc cagcccgctt cgttccacgc gccttcaccg cagagccgcg ccaccttgcc
     7801 atgcatgcac aacgcatcga tcttgacgtc cttggcgctg gcgcgaacct tgaccggcgt
     7861 atcgagctgc aggttgatgt tggcggtgtc gtcgatgaac aggtcgccga tggttccgtt
     7921 ccacacgccg tcggcgaagc cgcctttacc cgagaggtga aggctggtga ttcccgccag
     7981 ggcatcggcc cggatggtgt gttccgccgt agtgccatcg agcgtcgcat tgaactgcgt
     8041 caacgaccgt tcgccgacgt tcaggccgct gaccgcgacg tcggcgtgcg aaattctctg
     8101 gccgcgccag tcgaggtcga tgtttgcgga tagtttgtcg acgctcatcg tcccgctctc
     8161 gatgtcattg ccgcgggcgg tgagcttgat gctgggtgca tccgaagtgc cgccgatgcg
     8221 gcccgtggca tgcagctcgc cgctggcgcc ttctttcagc agcgccaggt tgtccgcgtc
     8281 gaggctgaag tcgagattga gtgcgcgcga tgaaccgatg tcaccgtcga tggccaggcg
     8341 cgtattaccc gcgcggaaac gcagcgcgtt gaaggtgtag ttgtcgccct ggagtttcac
     8401 gcggccactg ccggtggcgg tgttgccgcg aagtttgccg acgagatcgc tgaacgcgaa
     8461 gtcgatggcg ccgtcgccac caaacggagc gccactcgcc ttcatattga agtcgagagc
     8521 tccgttgaat ccaggtcgca gcctcgcggg attgattccc ttgaccttgc cttccagcga
     8581 ccagctttct tcgggactcc agcgcgcttc acccgcgagc gtcgccttgc cttcgaacgc
     8641 accgaccacg agatcatcca tctgcagatg atccttgtgc agcgcgccgc gcacggcgac
     8701 tgtcatggga tcgagttgtg gaatgaacag atcgccactg gcggtgatgg catagggcca
     8761 gaggccttcg agacggtatt tgccttcggg gctcgagaac agctgcggcg tttcctcagt
     8821 gaagcgcgcc acgagcggcc agcgcagacc acgccagtcg ccatagagaa ggagttgcgg
     8881 gccgttctcc gcgggctcga tggttccctg gccctcgacg tgactgccgg tggccttgtg
     8941 cgtcacttcg tagtgagtcg cgttgaccac gcggtccgcg tagttgccct cgaacaggat
     9001 gtcgaagggt ccgctgccga gcccgggcac catgagtggg ccgcgcgcga agaactcgtt
     9061 catctcgccg ccgacgtcga gcggcccttt gatgatgccg agcgcactgc cgccgccgaa
     9121 agcctgcagg tcgaagttgt gcacgtcggc cttgccggtc cagtggaagt tgctggacag
     9181 ggacagcaac tcgccgcgca tgtcggcgcg gaacggctcg aacagcttga gggtcagcgg
     9241 tagtttgtcg agcgtgcctt cgaaactcga atcggcacgc caggtgggct ggccttcgat
     9301 gatcatgcgc gtggtggctt cgccgctgag ctccatgatg tcggcggcgc gcagttcgcc
     9361 gatggcgcgc gcctgcagca cgccatacac gacgttgctg tcgaagacgc ggatcgtctt
     9421 gtgaccgacg gtcccggagc cggtgacgtt gtcgaactcc acgcgtttgc cgctgggcgc
     9481 gacgatgacg agagtgcgcg cgctggcctt ctcggcgctg atgctgagca ggcgcggcag
     9541 gaacctcgcc gtattgttgg gaggcggcgt gagccggcgc ctcacatcga tctgcacgac
     9601 atcggctcgt gcctcgcgta ccgcgatgcg gcccaccagg atgggccaaa aattgacgcg
     9661 ggccgagccg ttcacgacgc gaacgtggga ccgctcgtga tcgatgtcgg cgaggtcggc
     9721 cccgaatccg ccggcgatcg tgccgtgaat gttttcgatg tgcagcgtga ccttgcccat
     9781 gcgttcgggc aggcgcgcaa cggcgaagcg caagccggct tcggtgtaga gaacccaggc
     9841 gaggaacgcc aggattgcca gcaacaacaa cgcgacgacg agggccgcga tcttgagccc
     9901 tcgcttgatc acaatttcgg cgagaagttg aggtgcaggc gagggccggg ccgctcggtg
     9961 ctgcccgcgg gcgtggtcaa ggcctgggcg acgtccaggc cgacggaaac gacgggcagg
    10021 cgcagacgca cgccgatacc gacggagtac atgagcggat cgtcgaagct gtcgaatgcg
    10081 ttgccggcgt cggcgaacac ggccaccgcg aacttcgagc cgacatcgcg gacgacttcc
    10141 agcgacccgg cgaacaggtg tttgccgccg accttttcgt aaaccggatt gttgtcctcg
    10201 tcgaagagcg gctggttgtt ggcgtcggtg gcctgcgcca cgggtgacag ttcattgaag
    10261 ccgaagccgc gcacgctgcg atcgccgccg gcgaagaaac gctgggatgg cgcgagaccg
    10321 ctggtgctgt cgaccgcggt ggcgccgatg tcgccgcgca ccagcacgtg ccatttcggc
    10381 gccagatcga acacacgttc gccctgcatg cggatctgca ggaagttgga atcggatccg
    10441 agcgcgtcat gtgaaccgcg caactccgca tacaacgcgc ggctgaacaa cgcctcaccg
    10501 agatagttgc gcggtaccag cgagaagctg atgcccggga tcagcagcga ttgcgtgtcg
    10561 gtccggttgg atgcaatgaa ctcggattcc gcgtgcagca gttcgacgta ggtcactcgt
    10621 tgccagaagt gaccgaacca cggcccgcgc acgtgggtat agctcgggat gaaattgacc
    10681 ttgcgatc
//
LOCUS       DATDEU010000451         4688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2126771, whole genome shotgun sequence.
ACCESSION   DATDEU010000451 DATDEU010000000
VERSION     DATDEU010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126771"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VIV63_10805"
     CDS             <1..865
                     /locus_tag="VIV63_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125133.1"
                     /translation="ELHVLPWSPLYLTYSVAFVLCAIGAVLLRRFALAFDAAERARDE
                     LDERVREKTAELERNLTHTRDLEHERALGAERERILQDMHDGLGGHLVQALAIATSQT
                     VLRPLEEPLRTCLEELRLMVDSLEPVNGDLTSVLGSLRVRISRRLAHAGVHVNWNVED
                     LPEVADLGPKKVLGVARIVQEAITNALKHSGCSEITVHAGAVPGDGSQVEIVVADNGR
                     GMSESGSPGHGLASMRRRAAELAGTLAIESTAGGTRVRLGLPIAAGNRIGMSGQAPEL
                     GLPAAAQPSHS"
     gene            868..1533
                     /locus_tag="VIV63_10810"
     CDS             868..1533
                     /locus_tag="VIV63_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6125134.1"
                     /translation="METPLPSRVNVFVIEDDAVTRESLRVRIEAHPRLAVGAAVGTAA
                     EGQAALATRPDVLLVDLGLPDGSGIDVIRGALRRHPGLPIMVITVFGNEKRVVGAIQA
                     GAIGYLLKDDQTNEIGAAIEQLLQGGSPISPAIARHLIRHFQPAPAAPASATREALTA
                     REQEVLALAMKGFSYAEIAELLGLSPNTIASYTKHIYEKLAVSSRTEAIYEASRLGLI
                     ESR"
     gene            1680..2882
                     /locus_tag="VIV63_10815"
     CDS             1680..2882
                     /locus_tag="VIV63_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125135.1"
                     /translation="MNRKLLWLAMALNSGVALAARDGVIVGQGHIPTISVESNDGQSY
                     DRVRGPTTALAFNISGRCPSNHGVLGASARALHWPDSTGEADIGELRNTDRTMSQRLV
                     WVPHSLTPIEWHPSWKQKAIAACNANLAKQKTNGRTTQQVFAQRWVLPKVEIDRVEGT
                     LQCLKLESNGAGFDSDGGKSFTMLNSASVNIQCEKRMADDFKAESKPKSPVNDFTNGV
                     HVVQANLTVLPKTTQNGVCGITLSGVIETDVINATVTLFYRNNKGGTTPWRTVKTDHS
                     KTVFFSDYLDFSKPAGPSESAATPGGTPGGFAPNPGKKHSGYYQMIGKNLPFESNMAS
                     FNFDCAKAAPGGVTTAPNKPTVPPNKIVPPATPPPPPKGLKGQAPAPQPLLLPAIQRV
                     RDAELRKK"
     gene            2879..>4688
                     /locus_tag="VIV63_10820"
     CDS             2879..>4688
                     /locus_tag="VIV63_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125136.1"
                     /translation="MTMIPTDAAAVRFALLAMWMAASAPARADVIPVFASDETLNVAL
                     PPGAIAPGATLQLELDAYDIGAVASVADGRISIPVATLGLTAGEHTLRILSASENGDI
                     DTLAEHTLDVYQRPGVRASTQTWNVLLGSQYRLAQDPDEAFDGSDRGSSTAALQWNAA
                     TDRGTWIASGSFDTFYNSDRGIGSEQPLWQLPALQLKAGRKFDSGHVAIAMGDNETLP
                     GNLVFSNFNRRGLRLEAGGLDERFRAQAFTLHADPVTQLDAPLVPDDSEDGVLGAYSE
                     IAPFARHPDALSLSVSWLDGDSDLGGVGIATPGLESEAPLTFGGHAWDAALDSFVFGR
                     ALWMHGEYAKSRFDADGEQSGDPARDDDAQRLVIQLSSGGDFALAGIDQWSLGFEHLR
                     VGTHFFSLGNLMLPGDLDLRQLHASIYTHGLTLDLQALEQNTDVDDDPQRPRVDSDQQ
                     RITLGYTPATADPSAQPWRWLGVPSVSAGYESTANEQSAEDLLVAGYDLDNRQRTVSG
                     ALNFTHTRFSFGITAERVERDDRSQPLVVDDFVLYQPTPDSTETLLGLNLSWFASERL
                     TLAPQWQRSRIREQPDGETRDSDLWSLQLQASVIPEV"
BASE COUNT          877 a         1510 c         1519 g          782 t
ORIGIN      
        1 cgagcttcat gtgctgccct ggtcgccgct gtatctcacc tattccgtgg cgttcgtgtt
       61 gtgcgccatc ggcgcggtgc tgctgcgccg cttcgcgctc gcgttcgacg cggcagagcg
      121 cgcgcgcgac gaactcgacg agcgcgtacg cgagaagacc gccgaactcg aacgcaatct
      181 cacgcatacg cgggacctgg agcatgaacg cgcactggga gccgaacgtg aacgcattct
      241 tcaggacatg cacgatggac tcggcggaca cctcgtgcag gcgctggcca tcgccacgtc
      301 gcaaaccgta ctccggccgc tcgaagagcc gctgcggaca tgcctcgaag aactgcgact
      361 gatggtggat tcgctcgagc cggtcaacgg cgatctcacc agcgtgctcg gctcgctgcg
      421 cgtgcgaatc tcgcgacgcc tggcgcacgc cggtgtacac gtgaactgga acgtcgaaga
      481 ccttcccgaa gtggcggacc tgggtccgaa gaaggtgctg ggtgtcgcgc gcatcgtgca
      541 ggaagccatc accaatgcgc tgaaacattc cggctgcagc gaaataaccg ttcatgcggg
      601 cgccgtaccc ggcgacggct cgcaggtgga aatagtcgtg gccgacaacg gtcggggcat
      661 gagcgagtcg ggcagcccgg gacacggtct cgccagcatg cgccgcaggg cggccgaact
      721 cgcaggcacg ctcgcgatcg aatccacggc tggcggcact cgcgtgaggc tgggtctgcc
      781 gatcgcggcc ggtaacagaa tcgggatgag cggacaagcc ccggagctgg ggctaccggc
      841 agccgcgcag ccttctcata gttagtcgtg gaaactcctc ttccgtcccg ggtcaacgtg
      901 ttcgtcatcg aagacgacgc cgtgacgcgc gaatcgctgc gcgtgcgcat cgaggcgcac
      961 ccgcgcctcg cggtgggcgc cgcggttggc actgcggcag agggtcaggc ggcgctcgcc
     1021 acccggccgg acgtgttgtt ggtcgatctc ggcttgccgg acggcagcgg catcgacgtc
     1081 attcgtggtg cactgcgccg gcatcccgga ctgcccatca tggtcatcac ggtgtttggc
     1141 aacgagaagc gggtggttgg cgcgattcag gcgggagcca tcggctatct tctcaaggac
     1201 gaccagacca acgagatagg cgcggccatc gagcagctcc tgcagggtgg ctcgcccatc
     1261 agcccggcga tcgcgcggca tctgatccgg cattttcaac cggcgccggc cgcaccggcc
     1321 tccgccacgc gcgaagcgct caccgcgcgt gaacaggaag tgctggcact ggcgatgaag
     1381 ggtttcagct acgcggagat tgccgaattg ctgggcctgt cccccaacac gatcgcgtcg
     1441 tacaccaagc acatctacga aaagctcgcg gtgagctcgc gaactgaagc catctacgag
     1501 gcttcgcgtc tcggattgat cgagagccgt taacacagcc gcccgcggcc ggcaacccca
     1561 tcccgggtgt gcgagcggcg ctgccgcaat cccaaactac tccccgaaag gtttttgaac
     1621 cgcgggcctg cgccgttcga cggatggcac atggaggcgt tgtgcctgga ggagtcgtca
     1681 tgaacaggaa attgctgtgg ctcgcgatgg ctttgaacag cggcgtggcg ctggccgcgc
     1741 gtgatggtgt gatcgtcggg caggggcaca tcccgacgat cagtgtggaa tccaacgacg
     1801 gccagtcgta tgaccgtgtg cgcggaccta ctaccgcgct ggctttcaat atttcagggc
     1861 ggtgtccgtc caatcatggg gtgctcggag cgtcggcgcg cgcacttcac tggcccgatt
     1921 cgaccggcga agcggacatc ggcgaattgc gcaacaccga ccggaccatg tcgcagcgac
     1981 tggtgtgggt tccacattcg ctgacgccaa tcgaatggca tccgagctgg aagcagaagg
     2041 cgatcgccgc ctgtaacgcc aacctcgcca aacagaaaac caatgggcgg acgacgcagc
     2101 aggttttcgc gcaacgatgg gttctgccca aggtggagat cgatcgagtc gaaggcacct
     2161 tgcaatgcct gaaactcgaa tccaacggcg cgggtttcga ttccgacggc ggcaagtctt
     2221 tcaccatgct caacagcgcg tcagtgaaca tccaatgcga gaagcgcatg gctgacgact
     2281 tcaaggcgga gtcgaagcca aagtcgccgg tcaacgactt caccaacggc gtgcacgtcg
     2341 tgcaggcgaa tctcacggtc cttcccaaga ccacgcagaa cggcgtttgc ggaatcacgc
     2401 tgtcgggcgt gatcgaaacc gatgtgatca acgcgacggt cacgctcttc taccgcaata
     2461 acaagggtgg aacgacgccc tggcgcacgg tgaaaacgga ccacagcaag accgtgttct
     2521 tcagcgacta cctggatttc agcaagccag cgggcccttc ggaaagcgcc gcgacaccgg
     2581 gcggcactcc gggcggcttc gcgccgaatc cgggaaagaa gcacagcggc tactaccaga
     2641 tgatcggcaa gaaccttccg ttcgaatcga acatggcgag tttcaacttc gattgcgcca
     2701 aggccgcgcc cggtggagtg accacggcac cgaacaagcc gaccgtgccg cctaacaaga
     2761 tagttccgcc ggccacgccg ccgcctccgc cgaaggggct gaagggccag gcgcccgcgc
     2821 cgcaaccact gctgcttcca gccatccaga gggtgcgtga cgcggagctg cgaaaaaaat
     2881 gacgatgatt ccgacggatg ccgccgcggt gcgcttcgcg ttgctggcga tgtggatggc
     2941 cgcgagcgcg ccggcgcgcg ccgacgttat ccccgtattc gcctccgatg aaacgttgaa
     3001 cgtggccctg ccgcctggcg cgatcgctcc gggcgcgacg ctgcagctcg aactggatgc
     3061 atacgacatc ggagctgttg ccagcgtcgc ggatggccgt atcagcatcc ccgtcgcgac
     3121 gctgggcctg accgccggag aacacacact gcgaattctc tcggccagcg aaaacggaga
     3181 tatcgatacg ctcgccgaac acacgctcga tgtgtatcag cgtccgggcg tgcgtgcttc
     3241 gactcagacg tggaacgtgt tgctgggcag ccagtaccgt ctcgcgcagg atccggatga
     3301 agcgtttgac ggaagcgatc gtggcagcag taccgcggcg ctgcagtgga atgcggcgac
     3361 ggaccgcggc acctggatcg cctcgggatc gttcgacacc ttctacaaca gcgatcgcgg
     3421 catcggcagc gagcagccgc tctggcaact cccggcgctg caacttaagg ccggacgaaa
     3481 attcgactcc ggtcacgtgg cgatcgcgat gggcgacaac gagacgctgc cgggcaacct
     3541 ggtcttcagc aatttcaacc gtcgaggcct gcgcctggag gcgggtggtc tcgacgagcg
     3601 cttccgcgcg caggcgttca cgctgcatgc cgatccggtg acgcagctcg acgctcccct
     3661 ggtgcccgac gattcggaag atggggtgct cggcgcctat tcggaaatcg cgcctttcgc
     3721 gcgccatccg gacgctctct cgctgagcgt gagctggctc gatggcgaca gcgatctcgg
     3781 tggcgtgggt atcgcgacgc cgggcctcga atcggaagcg ccgctgacat tcggcggcca
     3841 tgcatgggac gcggccctcg acagtttcgt tttcggacgt gcgttgtgga tgcacggcga
     3901 atacgcgaaa tcgcgcttcg atgccgatgg cgagcagtct ggcgatccgg cgcgcgacga
     3961 tgacgcgcaa cgcctggtga tccagctttc gtccggcggc gacttcgcgc tcgccgggat
     4021 cgaccagtgg tcgctggggt tcgaacacct tcgcgtcggc acgcatttct tctcgctcgg
     4081 aaacctgatg ctgccaggcg atctcgacct gcggcagctg catgcctcga tctacacaca
     4141 cggcctcact ttggacctgc aggcgctcga acagaacacc gacgtggatg acgatcccca
     4201 gcgtccgcgc gtcgacagcg accagcagcg gatcacgctc ggttatacgc cggccacggc
     4261 cgacccctcg gcgcagccgt ggcggtggtt aggtgtgccg tcggtgagcg ccggctacga
     4321 gtccacggcg aacgagcagt ccgcggagga tctcctggtc gccggatacg acctggacaa
     4381 ccgccagcgc accgtttccg gcgccctgaa tttcacgcac acgcggttca gtttcggcat
     4441 caccgccgag cgcgtggagc gtgacgaccg ctcgcagcca ctcgtcgtcg atgacttcgt
     4501 gttgtaccag ccgacgccag actccaccga gaccctgctg ggcctgaacc tgtcctggtt
     4561 tgccagcgaa cggctgacgc tggcgccgca atggcagcgc tcgcgaatcc gcgagcagcc
     4621 cgacggagag acccgcgaca gcgatctgtg gtcgctgcaa ctccaggcga gcgtcatccc
     4681 cgaggtgt
//
LOCUS       DATDEU010000452         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1835184, whole genome shotgun sequence.
ACCESSION   DATDEU010000452 DATDEU010000000
VERSION     DATDEU010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835184"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /gene="purM"
                     /locus_tag="VIV63_10825"
     CDS             <1..735
                     /gene="purM"
                     /locus_tag="VIV63_10825"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY6125137.1"
                     /translation="AEPLFFLDYFATGKLEVDAAARVIEGIAAGCAEAGAALVGGETA
                     EMPGMYHGDDYDLAGFCVGVVERDRIIDGTKTAAGDVVIGLASSGPHSNGYSLIRKLV
                     SVAGADEKTQLDGKSLFDRLLTPTRIYVKAMLSLAQKVNVKGFAHITGGGLTDNIPRV
                     LPDGFEVALHRRNWPRDPVFDWLAATGKISAAEMYRTFNCGIGMIVVVAPADVDATLA
                     HLKASGENAFIIGGVGHGSRGVVIDE"
     gene            732..1394
                     /gene="purN"
                     /locus_tag="VIV63_10830"
     CDS             732..1394
                     /gene="purN"
                     /locus_tag="VIV63_10830"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY6125138.1"
                     /translation="MTAMPMPVTVVISGRGSNMEAIARTSLAAGSPFRIVRVIADRDS
                     AGGLARAASLGIPVSVVPFRQYPDRASFDAALAAEIEASGARLVVLAGFMRILSAEFV
                     QRLEGRLLNVHPSLLPKYKGLDTHARALAAGDSHHGASVHFVTAELDGGPVIMQGRLR
                     IRPGDTPDGVSARVHTLEHIIYPHVCTLIASGRIEWRAGSVFFDGAPLASPLIEENDA
                     AA"
     gene            1381..2085
                     /locus_tag="VIV63_10835"
     CDS             1381..2085
                     /locus_tag="VIV63_10835"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY6125139.1"
                     /translation="MRLRESLLVLVLLATGPVAHADPIDLKPFRATYVAEWKGMTAAS
                     STVELVRAGGDTYTYSSTNSARGMFRMAFPDALTQVSTFKIIDGRVIPQTFRGIDEKE
                     RPINLNFDWQAKRVTGVAKERPVDLELPEGTQDAMSLQIASLRNLASGNLKGTVWMID
                     STKLKEYELLLEGNARIETALGELDTVIYSSKRADSERMTRTWVAPTLGYLPVKAESI
                     RGKKVEVTLLIQTVDR"
     gene            complement(2224..>2325)
                     /gene="dcd"
                     /locus_tag="VIV63_10840"
     CDS             complement(2224..>2325)
                     /gene="dcd"
                     /locus_tag="VIV63_10840"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY6125140.1"
                     /translation="QMLFFESDEVCSTSYKDRGGKYQGQRGVTLPKT"
BASE COUNT          419 a          762 c          738 g          406 t
ORIGIN      
        1 gccgagccgc tcttctttct cgactacttc gccaccggca agctcgaggt cgatgcggcg
       61 gccagggtta tcgagggaat cgccgcgggc tgcgccgaag cgggcgccgc actggtgggc
      121 ggtgagaccg cggaaatgcc gggcatgtac cacggcgacg attacgacct cgcgggcttt
      181 tgcgtcggcg tggtggagcg cgatcgcatc atcgacggca ccaagaccgc ggccggcgat
      241 gtcgtcatcg gcctcgcctc ttccggcccg cattcgaacg gctattcgct gatccgcaaa
      301 ctcgtgtcgg tcgcgggcgc ggacgagaaa acccagctcg acggcaaatc cctgttcgac
      361 cgtttgctga ctccgacgcg catctacgtg aaggcgatgc tgtcgctggc gcagaaggtc
      421 aacgtgaaag gcttcgcgca catcacgggt ggcggcctca ccgacaacat tccgcgcgtg
      481 ctgcctgatg gcttcgaagt cgcattgcac cggcgcaact ggccgcgcga cccggtgttc
      541 gactggcttg ccgccaccgg caagatctcc gcggcggaga tgtatcgcac cttcaattgc
      601 ggcatcggca tgatcgtcgt ggtggcgccc gccgacgtcg acgcgacgct ggcgcatctc
      661 aaggccagcg gcgaaaacgc cttcatcatc ggtggcgtcg ggcacggttc gcgtggcgtg
      721 gtcatcgacg aatgaccgca atgccgatgc cggtgaccgt ggtcatctcc gggcgcggca
      781 gcaacatgga agccatcgcg cgcacctcgc tcgccgccgg ctcgccattc aggatagtta
      841 gagtcatcgc cgatcgcgac agcgccggcg gcctggcgcg tgcggcgtcg ctgggaattc
      901 cggtgagcgt cgtgccgttc aggcaatacc ccgaccgcgc cagtttcgat gccgccctcg
      961 ctgcggaaat cgaggcaagc ggtgcgcggc tggtggtgct cgccgggttc atgcgcatcc
     1021 tctcggcgga attcgtgcag cgcctcgaag gccggctgct caacgtccac ccgtcgctgc
     1081 tgccgaagta caaggggctg gacacgcatg cgcgcgcgct cgcggccggc gattcgcacc
     1141 atggcgccag cgtccacttc gtcacggcgg agctcgacgg cggcccggtc atcatgcagg
     1201 ggcggctcag aatccggccc ggggacaccc cggatggcgt ttcagcgcgc gttcatacgc
     1261 tggaacacat catctaccct catgtgtgta ccctcatcgc ttcaggccgc atcgaatggc
     1321 gcgccggttc cgtatttttc gacggcgcgc cccttgcgtc gccactcatc gaggaaaacg
     1381 atgcggctgc gtgagagtct cctggtacta gtgctgctgg ccaccggccc ggtggcgcat
     1441 gcggatccca tcgacttgaa accctttcgc gccacctacg tcgccgagtg gaaaggcatg
     1501 acggccgcga gttcgacggt cgagctggtg cgcgcaggcg gcgacaccta cacgtactcg
     1561 tcgaccaact ccgcgcgcgg catgttccgg atggcgttcc ccgacgcgct cacgcaggtc
     1621 agcaccttca agatcatcga cggacgcgtg attccgcaga cgttccgcgg catcgatgag
     1681 aaggagcggc cgatcaacct gaatttcgac tggcaggcca agcgtgtgac gggcgtcgcc
     1741 aaggaacgcc ccgtggacct ggagcttccc gaaggaaccc aggacgcaat gtcgctgcag
     1801 atcgcgtcac tgcgaaatct cgccagcggc aatctgaaag gcaccgtgtg gatgatcgac
     1861 tccaccaagc tcaaggaata cgaacttctg ctcgagggca acgcacgcat cgaaaccgcg
     1921 ttgggcgaac tcgacaccgt gatctactcg agcaaacgcg cggactcgga acgcatgacg
     1981 cgcacctggg tggcgcctac gctcggctat ctgccggtca aggccgagag cattcgcggc
     2041 aagaaggtcg aagtgacgtt gttgatccag acggtagata ggtaagaatg gggacagtcc
     2101 ccaattccga gcaaatgggg ccggacctca attaccgttg ggcggcgcca tcaacacttc
     2161 acttcgcagg tccagcccca tttgccgctt cgcggggaat tggggaccgt cccgctagct
     2221 aacctaggtc ttcgggagcg tcacgccccg ctggccctgg tacttcccac cccgatcctt
     2281 gtacgacgtc gagcagactt cgtccgactc gaagaacagc atctg
//
LOCUS       DATDEU010000453        11786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_375453, whole genome shotgun sequence.
ACCESSION   DATDEU010000453 DATDEU010000000
VERSION     DATDEU010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375453"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="VIV63_10845"
     CDS             complement(<1..1442)
                     /locus_tag="VIV63_10845"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY6125141.1"
                     /translation="MPTAFSRKVLLPLCIVSALAGCGGGGSDTPRPPPNAAPVLTSTT
                     FAATEDTAFAGQLTATDAENQAITFARTTDAQHGQVTISATGAVAYTPAANYAGADTF
                     GVRLTDSAGGTSTGTVTLNVAAVNDVPVFASPTLGTPEDVALDAQLTAYVTDPDANPL
                     TFAVTTAPAHGVLTLSAGGTAAFPPAANYNGADSFAISVSDGAGGIATGTVTINITAV
                     NDAPVLTTTQLSIAEDGVLTAQLATTDVENQVTQYQVGGGAGHGQVSISPTGFLTYTP
                     DANFNGADSLLVGVSDDGATLTLWPLDITVSPVNDAPVAYEDLLRLPATATVTLPVLA
                     NDSDADGDTLTVTLLSQPGGGTLTVNASNEITFTRDNQFNGPIRFSYRITDAAGVTAE
                     ADVRAVIGEFPGIYYLADETTVGQLEVHWFDGLRIYRLGTDLDAGDVISSFATAGDGD
                     TVAYVVDSDTVSRVFFTGPTAQDSRVVFTSG"
     gene            1585..1659
                     /locus_tag="VIV63_10850"
     tRNA            1585..1659
                     /locus_tag="VIV63_10850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1617..1619,aa:Val,seq:gac)
     gene            1813..3501
                     /locus_tag="VIV63_10855"
     CDS             1813..3501
                     /locus_tag="VIV63_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEY6125142.1"
                     /translation="MSIELNGLPDEQRDAVRASLKLNNYDKRDISASEVRSAYKDADE
                     QIRQALEPFGYYDSKVTKQLSGDATNGWKAKFDVIPGEPTIVREVHVEVNGEGKELRR
                     VESAVAAFVPKVGDRLDHATYEASKSIIDTSLRGAGFLDAKFLRRRVTVKPEEVSATV
                     DLAWESGPRYKFGAVRFDGDAPFTEDFLRDFIPWREDAFFNSEQVLNLQQRLVDADYF
                     QLVSVQPSFDEKKDGTVPIDVLLKRDERTVYSGEVYYSTDFGAGVRVGADRRWLNKKG
                     HKAGAKVEYSERLQEAAVHYKIPRPGRNDRSYDYGVGYRDETTDVTRSRNFQLAATRS
                     ERIGRGYTRTIGLKYLDGDFEIGQDEDNVEYGSSALLFIEATLSRRRVNDRLSPRKGY
                     VIEVGTRLSSEAVASDTDLAQAFGRLTWLMPQGDRGRLKIRGEVGAMTVGDFDALPPE
                     LRFYAGGDRSVRGFDYHEIGEVNENGNIIGGKFLAVASAEYEYYFKEDWGAAVFVDAG
                     DAFTSTFSVNVGAGVGLRWRSPLGPIRIDVGFPVHTDLPTESSWRLHVLLGPDL"
     gene            3498..6839
                     /locus_tag="VIV63_10860"
     CDS             3498..6839
                     /locus_tag="VIV63_10860"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY6125143.1"
                     /translation="MKRVLKWSALSLAVLLLLVAGLVSWLLFTNSGARWVAATVTERY
                     LPALSYSKLEGTIASSITLVDFAFDGGAEGVKVRAARIHVEPTLSALFGRTLHVNRAS
                     VQTLDVLLPPAKEEPDEPDKPLWVAAPLLVIVDEFSIEKAIIRRDTGVLTRVRKLELA
                     ARWSAEELLLRRLAVESDDLRGSLLVNGTIKPEQNTVRAKLEARWQDIVVPESMAGRV
                     LASRGAVNIDGTPKAYAVKGELDIGPPEELVHVLIDASGTDKRADLRQLELRQTAGHL
                     TLSGTVEFEPVAWSLYAKASDFNPGALLAEWPGKLDIDASTRGVLAEAGPRGELQIAT
                     LSGTLRGRPIAGEADLEFVAPSRLTGNLRISSGKSRISVKGNAADAKRIDATVELAVA
                     SLNDWVPKTQGSLTGRFTVRGGWPRLTIAGSADGKSLGMGENIVAKLHVDATVESPLD
                     PNGKVKAVASQVTLGGQLFTQVTLDASGNQAKHRVAVSADSEQFDGSIELAGGLTKGG
                     WSGELADLEFKAADLANLSLREPSRVVYDAGSFSLLQTCLEDKSTVLCSALKFEASGA
                     LDAGYSFERVPLKLANTLAPDALSGELQGEVQGHGRVRRGADGQWIGDLAVTSESARL
                     VMAEGEEIPAAAALANEGSLLIYENLDAQVDFAGMKANAKLTARLAHGGSISATLTAS
                     DLNAPAPRIAGNISAAMPTLAPFGAFVPAVANLDGAVDAKIEIGGTTAAPEFTGNVDA
                     TKLQADLGSIGIELREGAVHGEALRLGGFKLAGTVASGKGKLEFNGTMDEHGVVDVKV
                     SGQDFLAADIPAANVIVTPDLTLTGDPKGYLLRGEVHIPRAAINLQKLPQGEPPGVSP
                     DVVVIRNGKEVENAAKAAALPLQAVITVHMGEKIEVTGYGLEATVSGQLTVRESPGVP
                     TSGSGQLGIAGRYRAYGQDLTIKDGRLLFAGTPLDNPRLAIVAMREVDDVSTGLKIGG
                     SAKRPVITVVSAPNMGEADSLSYLVTGRSLSDVGSASGSSQDALASATRSLEGTGAGL
                     VAQRIGARLGLDEAGVEENELIGGSALTIGEYLSPRLYLSYGVGLFEPGEVIALRYKI
                     SKDVGVRVQRGTQETRAGVEYRIER"
     gene            complement(6864..9452)
                     /gene="ligD"
                     /locus_tag="VIV63_10865"
     CDS             complement(6864..9452)
                     /gene="ligD"
                     /locus_tag="VIV63_10865"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY6125144.1"
                     /translation="MADAGHKARGKTRLERYVAKRDFKKTAEPSEKARAAKGKKLSFV
                     VQKHAARRLHWDFRLEWEGTLRSWAVPKGPSLDPADKRLAVEVEDHPIAYAKFEGDIP
                     KGQYGGGHVDIWDNGTWEPVGDFAKGLKKGHLEFEMFGKKLSGRWHLVRTRMQGKQPN
                     WLLMKSGDFAARAGANADVIDAGGADDPKRMLRPRRVKPAMVPRPVPRGAPKPRSTKR
                     KGTRGSALPATIKPMLATLVDKVPGDDDWVYELKYDGVRLLCRCDGEDVRCISRNGLD
                     WTHKTGPVVEALARLKLDGAWVDGELIVTDPNGRSDFSLLQHTMEQGRLDELQFCIFD
                     LLFNRGEDLRALPLSERKVRLDAAFAKLPASGPLRLADQIQSDSAELLARVCHQHLEG
                     LVAKKIDSRYTGDRSENWLKVKCHREQEFVVGGAAFLPGRGTGTFSSLLVGVKSGAGL
                     KYAGRVGGGFDAHERAEWHARVKKLARKDSPFDRLPEKRPGEIWHWMKPELVIQVAFA
                     DWTHGGILRQPRYLGLRQDRDPKTVVREEPEHIEDVVKPKKKGKARAKTAAPPDDEVP
                     ASLGRLTHPERVLYPHDGITKLQLARYFDAIGEQAMPHYKDRPLSILRNTHGSKPFFQ
                     KHFLEKSGTGLRVVKIPNADKDPDFTVCDSPEGLVHLAQVGAVELHSWGAVMPRPTQA
                     DRLTFDLDPGADLPYPPLRDAALAIRKLMKDVGLESWVKTTGGKGLHVVVPLTAPRPN
                     WDDAKEFARSVTLFMERLAPTMFTSKTGERNRKNKIFVDYLRNGFGATAVAAFSPRWR
                     PGVGVSTPVAWDELDDDIRGTHFDIHNVPDRIAQQRKDPWKDYWTAKQALTKAMLKKM
                     GSDPIS"
     gene            complement(9456..10403)
                     /locus_tag="VIV63_10870"
     CDS             complement(9456..10403)
                     /locus_tag="VIV63_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY6125145.1"
                     /translation="MAKSKQTGNGGDVEKPRRGARGLWNGAISFSLIHIPVSLHTAAR
                     AHELDLNLLDKRDFAPVGYQRYNKATGKVVEWNDVVKGYEYEKGEYVVLTDEDFRRAN
                     VEASRTIDIQTFVDRDAIAPYYFDTPYFLVPDKNGERVYALLREALEQSKKLAVATFV
                     LRSRQHVAALMPVDKIIVLNTLRYEQEIQPHPEVAAAVAKKANAAGGRELTMALKLVD
                     EMSEKWKPEVFTDTYRDDLLKRIQQKVKAGQTHTLTEPEAETTERAPAGGKVVDLMSL
                     LQRSLDQKGGAAETATRATKRKHSAARRRSTNARAARRA"
     gene            complement(10396..11517)
                     /locus_tag="VIV63_10875"
     CDS             complement(10396..11517)
                     /locus_tag="VIV63_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6125146.1"
                     /translation="MKSPSRSVRPVRFAVVGLGHIAQAAVLPAFKHARPHAELTALVS
                     DTPAKLKTLGRRYGVKHLYSYDDSRDLFSSGEVDAVYIALPNTQHAAWTIRAAEAGLH
                     VLCEKPLATSVIDCERMIEACERNRVQLMTAYRLHFERCNLEVADLVRKKRIGEARYF
                     DAQFSMQVKPGNIRLKRDLGGGPEWDIGIYCQNAARYVFADEPTQLWATTTHSGDRRF
                     GEVPETVHVILKFPGERIANFICSFGAADRSRYEIVGTKGSIVCDPAFDYAEGLSYEL
                     TIGEKKQRKKFSKSDQFAPELVHFAKCIRANRRPEPSGIEGLIDVAIIDAIHRSIGTG
                     GWAAVEAPQKRRRPSLRQEMRKPAVPREPPLVQAESGHS"
     gene            11652..>11786
                     /locus_tag="VIV63_10880"
     CDS             11652..>11786
                     /locus_tag="VIV63_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017402730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEY6125147.1"
                     /translation="MPTITLPDGSQKNFETPVSVADVAASIGAGLARAAIAGRVNDKL
                     V"
BASE COUNT         1986 a         3597 c         4009 g         2194 t
ORIGIN      
        1 ccgctcgtga agacgactcg cgagtcctgg gcggtggggc cggtgaagaa cacgcgcgag
       61 acggtgtcgc tgtcgaccac gtaggccacg gtgtcgccat cgcctgcagt cgcgaacgag
      121 ctgatgacat cgcccgcgtc gagatccgtg ccgagccggt agatgcgcag accgtcgaac
      181 cagtggactt cgagctgccc caccgtggtt tcgtcggcca ggtaataaat gcccgggaat
      241 tcgccgatca cggcgcgtac atcggcttcg gcggtaacgc ccgcggcgtc agtgattcga
      301 tagctgaagc ggatgggccc attgaactgg ttgtcgcgcg tgaacgtgat ttcgttcgac
      361 gcattgaccg tgagcgtgcc gccgccaggt tgggaaagaa gggtgacggt gagcgtgtcg
      421 ccatcggcat cgctgtcgtt ggcgagcacg ggcaacgtta ccgtggcggt ggccggcagt
      481 cgcagcaggt cttcgtaggc gaccggtgca tcgttgaccg gggatacggt gatgtcgagt
      541 ggccagagcg tgagcgtggc gccgtcatcg gacacgccga cgaggagact gtccgcgcca
      601 ttgaagtttg catcgggcgt gtaggtgagg aatccggtcg gcgagatgga cacctggccg
      661 tgtccggcgc cgccgcccac ctggtactga gtgacctggt tctcgacatc ggtcgtcgcg
      721 agctgggccg tcaacacacc gtcttcggcg atgctgagct gcgtggtggt gaggaccggg
      781 gcgtcattga ccgcggtgat gttgatcgtg acggttcccg tcgcgatgcc gccggcgccg
      841 tcgctgacac tgatcgcgaa ggaatccgcg ccgttgtagt tcgcggccgg cgggaaagcc
      901 gcggtgccgc ccgcggacaa ggtgagcacg ccgtgagcag gcgcggtcgt caccgcgaag
      961 gtcaggggat tcgcgtcggg atccgtgacg taggccgtga gctgcgcgtc gagcgccacg
     1021 tcttcgggcg tgcctagcgt cggcgatgca aataccggta cgtcgttgac ggccgcgacg
     1081 ttgagcgtga cggtgccggt ggacgtgccg cccgcgctgt cggtcagccg gactccgaaa
     1141 gtgtccgcgc ccgcgtaatt ggccgccggg gtgtatgcca ccgcgcccgt ggcggaaatg
     1201 gtgacctggc cgtgctgcgc atcggtcgtc cgtgcgaacg tgatggcctg gttctcggcg
     1261 tccgtggccg tgagctgccc ggcgaatgcc gtgtcttccg tggccgcgaa cgtggtcgac
     1321 gtcaggaccg gcgcggcatt cgggggcgga cgaggcgtgt cggaaccgcc gccgccgcaa
     1381 cctgccaggg ctgaaacaat gcacaggggg agcaggactt ttcgggaaaa cgcggtcggc
     1441 atgaaatcgt cctcttgggt gcagtttggt ctgtcggtca gaaagactaa cgattaaggg
     1501 gggttacgcg ggtctgtctt tgacttcttg ccgcaaaaaa cctcgtcctg tatcgttcgc
     1561 gccctcgttc cggggcattt cggcagtcgc gtagctcagt ggtagagcac ctccttgaca
     1621 tggaggtggt cggtggttcg atcccactcg cgactaccag tccccttaca gacgcccggc
     1681 gcgccggtgg tgaacctagc cacatgttca agatctctct gccgctgaag gccgttttct
     1741 gacggacgtc ctctggtgag gacgctggcg ttaaccgtct tactcatctc gacctccgct
     1801 tccggcgccg gggtgagcat tgagctcaat ggactgcccg acgagcagcg cgacgccgtg
     1861 cgcgcatcgc tcaaactcaa taactacgac aagcgcgaca tctccgcgtc cgaggttcgc
     1921 tcggcttaca aggatgccga cgagcagatc cgccaggccc tcgagccgtt tggatactac
     1981 gactcgaagg tgaccaaaca actttccggc gatgcgacca acggctggaa agcgaagttc
     2041 gacgtgatcc ccggcgaacc gaccatcgtt cgcgaagttc acgttgaagt gaacggtgaa
     2101 ggcaaggagt tgcggcgcgt cgagtcggcg gtggcggcat tcgtaccgaa ggtcggcgac
     2161 cggctcgatc acgcgaccta tgaagccagc aagtcgatca tcgacaccag cctgcgcggc
     2221 gcgggttttc tcgacgcgaa attccttcgc cgccgcgtga ccgtgaaacc ggaagaggtt
     2281 tcggccacgg tggacctcgc ctgggaaagc ggtccgcggt acaagtttgg cgccgtgcgt
     2341 ttcgacggcg acgctccgtt cacggaagac ttccttcgcg acttcatacc gtggcgcgag
     2401 gatgcgttct tcaattccga gcaggtgctc aatctgcagc agcgcctggt cgatgcggac
     2461 tacttccagc tcgtgtcggt gcagccgtcg ttcgacgaga agaaggatgg gaccgtgccc
     2521 atcgacgtgt tgctgaagcg cgatgagcgc accgtgtatt ccggcgaggt ctactacagc
     2581 accgacttcg gcgccggcgt gcgcgtcggc gcggaccgcc gctggctcaa caagaaaggg
     2641 cacaaggccg gcgcgaaggt ggagtactcg gaacgcctgc aggaggcggc ggtgcactac
     2701 aagattccgc ggccgggacg caacgatcgt tcttatgact atggcgtcgg ttatcgcgac
     2761 gagaccacgg acgtcacgcg gtcgcgcaat ttccagttgg ctgcgacgcg ctccgagcgc
     2821 atcggccgcg gctacacgcg caccatcgga ctcaaatatc tcgatggcga tttcgagatc
     2881 ggccaggacg aagacaatgt cgagtacggc agctccgcgt tgttgttcat cgaagccacg
     2941 ctgagccggc gccgggtcaa tgaccggctg tcaccgcgca agggatacgt gatcgaggtc
     3001 ggcacccggc tgtcctcgga ggcggtcgcc tcggacaccg atctggcgca agccttcggc
     3061 aggctgacct ggttgatgcc gcaaggcgat cgtggccggc tgaagatacg cggcgaagtc
     3121 ggcgccatga cggtcggtga cttcgatgcc ttgccgcccg agctgcgctt ctacgcgggt
     3181 ggcgaccggt ccgtgcgcgg tttcgactac cacgagatcg gcgaggtgaa tgagaacggc
     3241 aacatcatcg gcggcaagtt cctcgccgtg gccagcgccg aatacgagta ctacttcaag
     3301 gaagactggg gcgcggcggt gttcgtcgat gcgggcgatg ccttcacgag caccttcagc
     3361 gtgaatgtcg gcgcgggagt cggattgcgc tggcgttcgc cgctgggacc catccgcatc
     3421 gacgtcggat ttccggtgca cacggatctg ccgacggaaa gcagctggcg cctgcacgtg
     3481 ctgctggggc ccgacctgtg aagcgcgtat tgaaatggtc ggcgttgtcg ctggcggtgt
     3541 tgttgctgct ggttgccgga ttggtttcct ggctgctgtt cacgaattcc ggggcacgct
     3601 gggtggcggc gacggtcacc gagcgctatc tacccgcgtt gagttactcg aagctcgaag
     3661 gaacgatcgc cagttcgatc acgctcgtcg acttcgcgtt cgatggcggg gccgagggcg
     3721 tgaaagtgcg tgcggcgcgc atccatgtcg agccgacatt gtcggcgctg ttcgggcgta
     3781 cgctgcatgt caaccgtgcg agcgtgcaga cgctcgacgt attgctgccg ccggctaaag
     3841 aagaaccgga tgagccggac aagccgttgt gggtggcggc accactgctc gtcatcgtcg
     3901 atgaattctc gatcgagaag gcgatcatcc gccgcgatac cggcgtgctg acgcgcgtgc
     3961 gcaagctcga actcgccgcg cgatggagtg cggaagaact gctgctgcgc cggctcgccg
     4021 tggaatcgga tgacctgcgc ggttcgttac tggtgaatgg cacgatcaag ccggagcaga
     4081 acaccgtgcg cgcgaagctc gaggcgcgct ggcaggacat cgtcgttccc gaatccatgg
     4141 ccggccgtgt gctggcgagc cgcggcgccg tgaatatcga cggcacgccg aaagcctatg
     4201 ccgtgaaggg cgagctcgat atcgggccgc ccgaagaact ggtgcacgtg ctcatcgatg
     4261 cgtcgggcac ggacaagcgc gcggatcttc gccagctcga gctgcggcag accgcagggc
     4321 atctgacgct aagcggcacg gtggaattcg agccggtggc gtggtcgttg tatgcgaagg
     4381 cgagcgattt caatccgggc gcattgctcg ccgaatggcc cggaaagctg gatatcgacg
     4441 cgagcacgcg cggcgtgctg gccgaagccg gcccgcgcgg agaactgcag atcgcgacgc
     4501 tctcgggcac gttgcgaggc cggccgatcg cgggcgaggc cgatctcgag ttcgtcgcgc
     4561 cgtcgcgtct cacgggcaac ctgcggatca gttctggcaa gagccgcatc tcggtgaagg
     4621 gaaatgcggc ggatgcgaag cgtatcgatg ccaccgtgga actggcggtg gcgtcgctca
     4681 acgactgggt gcccaagacc cagggtagtt tgacgggtcg cttcaccgtg cgtggaggat
     4741 ggcccaggct caccatcgcc ggcagcgccg acggcaagtc gctcggcatg ggcgagaaca
     4801 tcgtcgcgaa gctgcacgtg gatgcgacgg tggaaagccc gctcgatccc aacggcaagg
     4861 tcaaggcggt ggcatcgcag gtgaccctgg gagggcagtt gttcacccag gtcacgctcg
     4921 atgcatccgg caaccaggcc aaacatcgcg tggcggtcag tgcggatagc gagcagttcg
     4981 acggttcgat cgaactcgcg ggcggcctca cgaaaggcgg ctggagcggt gaactggcgg
     5041 atctcgagtt caaggcagcg gaccttgcca atctgtcgct gcgcgagccc tcacgcgtgg
     5101 tgtacgacgc gggcagtttc tcgctgttgc agacctgcct ggaagacaag tccacggtgt
     5161 tgtgctcggc cctgaaattc gaagcgtcgg gcgcgctgga tgcggggtac tccttcgagc
     5221 gcgtgccgct caagctcgcg aacacgctgg cgccggatgc gctgtccggc gagttgcaag
     5281 gcgaggtgca ggggcatggt cgcgtgcggc ggggtgcgga tgggcagtgg ataggggatc
     5341 tcgcggtgac gtccgaatcc gcgcgcctcg tcatggcgga aggcgaggag attcctgcgg
     5401 cggcggcgct ggccaacgag ggttcgctgc tgatctacga gaacctcgac gcgcaggtgg
     5461 acttcgcggg catgaaggcg aatgcgaaac tcaccgccag gcttgctcat ggcggcagca
     5521 tcagcgccac gctcacggcc tcggacctga atgcaccggc gccgcgcatc gcggggaaca
     5581 tcagcgccgc gatgccgacg ctggcgccgt ttggcgcgtt cgtgccggcc gtggcgaatc
     5641 tcgatggcgc cgtcgacgcg aagatcgaaa tcggtggcac gacggctgcg ccggagttca
     5701 cgggtaacgt ggatgccacg aaattgcagg cggatctcgg gagcataggc atcgaattgc
     5761 gcgagggcgc ggtgcacggc gaagcacttc gcctgggtgg attcaagctc gccggcaccg
     5821 tggcctcggg caagggaaag ctcgaattca acggcacgat ggacgaacac ggtgtcgtgg
     5881 atgtcaaggt gtcgggacag gatttcctgg cggcggatat tccggcggcc aatgtcatcg
     5941 taacgccgga cctcacgctc acgggcgatc cgaagggcta tctactgcgc ggcgaggtgc
     6001 acattccgcg cgcggccatc aacttgcaga agttgccgca aggcgagccg ccgggcgtgt
     6061 cgccggacgt cgtggtgatc cgcaacggca aggaagtcga gaacgcggcg aaggcggcgg
     6121 cgttgccgct gcaggcggtc atcaccgtgc acatgggcga gaagatcgaa gtcacgggtt
     6181 acggtctcga ggcaacggtg tcggggcagc tcacggtgcg cgagtcgccc ggggttccga
     6241 cgtcgggctc gggacaactc ggtattgccg gcaggtacag ggcttatggc caggacctca
     6301 ccatcaagga cggccggctg ttgttcgcgg ggacgccgct cgacaatccg cggctcgcga
     6361 tcgtcgccat gcgcgaggtc gacgatgtgt ccacggggtt gaagattggc ggctcggcga
     6421 agcgcccggt catcacggtg gtgtcggcac ccaacatggg tgaagcggat tcgctgtcgt
     6481 acctggtcac tggacggtcg ctgtccgatg tcggcagcgc gagtggcagt tcgcaggatg
     6541 cgctcgcgtc ggcgacgcgg tcgctcgagg gtactggcgc ggggctcgtg gcgcagcgca
     6601 tcggtgcgcg gctcgggctc gacgaagccg gcgtggaaga gaacgaactg atcggtggct
     6661 cggcgctgac catcggcgag tatctgtcgc cgcgtttgta cctgagctac ggcgtcggat
     6721 tgttcgagcc gggcgaagtc atcgcgctgc gctacaagat atcgaaggac gtgggtgtcc
     6781 gcgtgcagcg tggcacccag gagacgcgcg ccggcgtcga gtaccgcatc gagcgataac
     6841 ggaaatgggg tcaggcccca ttttcaggaa atggggtctg accccatttt cttgagcatg
     6901 gctttcgtca gggcctgctt cgcggtccag tagtccttcc acgggtcctt gcgctgctgg
     6961 gcgatacggt cgggcacatt gtggatatcg aaatgcgttc cgcggatgtc gtcgtccagc
     7021 tcatcccagg cgacgggcgt cgacacgccg acaccgggac gccagcgtgg tgaaaacgcg
     7081 gccacggcgg tggcgccgaa accattacgc agatagtcca cgaagatctt gttcttgcgg
     7141 ttccgctcgc ccgtcttcga cgtgaacatc gtgggggcga gccgctccat gaacagcgtg
     7201 acgctgcgcg cgaattcctt cgcatcgtcc cagttcggac gcggcgccgt caggggcacg
     7261 accacatgca gacctttgcc gccggtggtt ttcacccagc tttcgaggcc cacatccttc
     7321 atgagcttgc ggatggcgag cgcggcgtcg cgcagcggtg gatagggcag gtccgcaccc
     7381 gggtccagat cgaacgtgag ccggtcggcc tgcgtgggcc gcggcatcac cgcgccccag
     7441 ctatgcagct cgacagcgcc cacctgcgca agatgcacca ggccctcggg cgaatcacac
     7501 accgtgaaat ccggatcctt gtccgcattc ggaatcttca ccacgcgcaa gcccgtgccc
     7561 gacttctcga gaaagtgctt ctggaaaaac ggcttcgatc catgcgtgtt gcgcaggatg
     7621 ctcagtggcc ggtccttgta atgcggcatg gcctgttcgc cgatggcgtc gaaataccgg
     7681 gcgagctgta gtttggtgat gccgtcgtgc ggatacagca cgcgctcggg atgcgtgagg
     7741 cggccgagcg aagcaggaac ctcgtcatcc ggcggcgcgg cggttttggc cctggctttt
     7801 ccctttttct tcggcttcac gacgtcctcg atgtgttcgg gctcctcgcg aaccacggtc
     7861 ttcggatcac gatcctgccg cagtccgaga taacgcggct ggcgcaagat gccaccatga
     7921 gtccagtcgg cgaaagccac ctggatgacg agctccggct tcatccagtg ccagatctca
     7981 cccgggcgtt tctccgggag ccggtcgaac gggctgtcct tgcgtgcgag ctttttcaca
     8041 cgcgcatgcc actcggcgcg ctcgtgcgcg tcgaaaccgc caccgacgcg gcccgcgtac
     8101 ttgaggccag cgccggattt cacgccgacc agcagcgacg agaacgtgcc cgtgcctcgg
     8161 cccggcagaa acgcggcgcc gcccaccacg aactcctgct cgcgatggca tttgaccttg
     8221 agccagttct ccgagcggtc gccggtgtag cgggagtcga tcttcttggc gaccaggcct
     8281 tccaggtgct gatggcagac gcgcgccagg agctcggcgc tgtcgctctg gatctgatcc
     8341 gcgaggcgca ggggcccgct cgcgggtagt ttggcgaatg ccgcatccag ccgcaccttg
     8401 cgttccgaga gcggcagcgc gcgcaggtcc tcaccgcggt tgaacagcag gtcgaatatg
     8461 cagaactgga gttcgtcgag ccggccttgc tccatcgtgt gctggagcag cgagaaatcg
     8521 gaacggccgt tgggatctgt cacgatgagc tcgccatcga cccaagcgcc atcgagcttg
     8581 agtcgggcga gcgcctcgac caccggcccg gtcttgtgcg tccagtcgag gccgttgcgc
     8641 gagatgcagc gaacgtcctc gccatcacag cggcacaaca gccgcacgcc atcgtatttg
     8701 agctcataga cccagtcgtc atcgcccggc accttgtcga ccagcgtcgc gagcatgggc
     8761 ttgatcgttg cgggcagggc gctgccacgc gttcccttgc gcttggtgct gcgcggcttc
     8821 ggcgcgccgc ggggcaccgg ccgcggaacc atggcgggct tcacgcggcg aggcctcagc
     8881 atccgctttg gatcgtccgc gccacccgcg tcgatgacat cggcgttggc gccggctcgc
     8941 gccgcgaagt cgccgctctt catgagcagc cagttgggtt gtttgccctg catgcgcgtg
     9001 cgcacgagat gccagcggcc ggacaacttc ttcccgaaca tctcgaactc gagatgcccc
     9061 ttcttcaagc ctttcgcgaa atcgccgacc ggctcccagg tgccgttgtc ccagatgtcg
     9121 acgtgcccac cgccgtactg gcccttggga atgtcaccct cgaatttggc ataggcgatc
     9181 ggatggtctt cgacctcgac cgccagacgc ttgtccgccg gatcgagcga gggccctttg
     9241 ggcacggccc agctgcgcag cgtgccttcc cattcgaggc ggaaatccca gtgcagtcgc
     9301 cgggccgcgt gcttctgcac gacgaaggag agcttctttc ctttcgccgc acgcgccttt
     9361 tcgctgggct cggcagtttt cttgaagtcg cgcttggcga cgtaacgctc gaggcgcgtc
     9421 ttgccgcgag ccttatgccc cgcgtcggcc atacatcacg cgcgtcgggc ggcgcgtgcg
     9481 ttcgtactgc gccggcgcgc cgcggagtgt ttgcgcttgg tggctcgcgt ggcagtttcg
     9541 gccgccccac ctttctgatc gagacttcgc tgcagcagcg acatcaggtc cacgaccttg
     9601 ccgccggccg gtgcgcgctc cgtcgtttcg gcttcgggct cggtcagcgt gtgcgtttgt
     9661 ccggccttca ccttctgctg gatacgcttc aggagatcgt cgcgatacgt gtccgtgaac
     9721 acttcgggct tccacttctc gctcatctcg tcgacgagtt tgagcgccat ggtcagttcg
     9781 cgcccaccgg ccgcattcgc cttcttcgcg acggccgccg ccacttccgg atgaggctga
     9841 atctcctgct cgtaacgcag cgtgttcagc acgatgatct tgtcgaccgg catcaacgcc
     9901 gcgacgtgct gtcggctgcg cagcacgaac gttgccacgg cgagtttctt cgattgctcc
     9961 agcgcttcgc gcagcagcgc gtagacgcgt tcgccgttct tgtcgggcac caggaagtag
    10021 ggcgtgtcga agtagtaagg cgcgatcgcg tcgcgatcga cgaaggtctg gatgtcgatg
    10081 gtccgagacg cttcgacgtt ggcgcgccgg aagtcctcgt ccgtgagcac cacgtattcg
    10141 cctttctcat attcataccc ctttacgacg tcgttccact cgaccacctt gccggtggcc
    10201 ttgttgtagc gctggtaccc gaccggcgcg aagtcgcgct tgtcgagcag attgagatcg
    10261 agctcgtgcg cgcgcgccgc ggtgtgcagg ctcacgggga tatggatgag actgaagctg
    10321 atggcgccat tccagaggcc gcgcgcaccg cgcctgggct tctcgacgtc gccgccattt
    10381 ccagtttgtt tggatttagc tatggccgct ctccgcttgt accaatgggg gctcgcgcgg
    10441 caccgccggc ttgcgcattt cctgtcgtaa ggatggccgc cggcgcttct gaggcgcttc
    10501 gacggcggcc caaccacccg ttccgatgga gcgatggatg gcatctatga tcgcgacgtc
    10561 gatcagtcct tcgatgcccg aaggctccgg acgccgattg gcgcggatgc acttcgcgaa
    10621 gtgcacgagc tcgggcgcga actgatcact cttcgaaaac ttcttgcgtt gtttcttctc
    10681 gccgatcgtc agctcgtaac tcaagccttc ggcgtagtcg aaagccgggt cgcagacgat
    10741 gctgcccttg gtgccaacga tctcgtaacg cgagcggtcg gcggcgccga agctgcagat
    10801 gaaattggcg atgcgttcgc ccggaaattt caggatcacg tgcacggttt ccggcacttc
    10861 gccgaagcga cggtcgccac tgtgggtggt cgtggcccac aactgcgtgg gctcgtcggc
    10921 gaacacatac cgggcggcgt tctggcagta gatgccgatg tcccattccg ggccgccgcc
    10981 gagatcgcgc ttgaggcgga tgtttccggg tttcacctgc atcgaaaact gcgcgtcgaa
    11041 atagcgcgcc tcgccgatgc gcttcttgcg cacgagatcg gcgacttcaa gattgcagcg
    11101 ttcgaagtgc aggcgatacg cggtcatgag ctgcacgcgg ttgcgctcgc aggcctcgat
    11161 catgcgttcg cagtcgatga ccgaggtggc gaggggtttc tcgcagagga catgcaggcc
    11221 ggcttccgcc gcgcggatgg tccaggcggc gtgctgggta ttggggagcg cgatgtaaac
    11281 ggcgtcgact tcgccgctcg agaagaggtc gcgcgaatcg tcatacgaat agaggtgttt
    11341 cacgccatag cgacggccca gcgttttcag cttggcgggc gtatcggaga ccagggcggt
    11401 cagttcggca tgcggccggg cgtgtttgaa agccggcaga acggcggcct gggcgatatg
    11461 gccgagtccg accaccgcga agcgcaccgg acgtactgat ctggaaggtg atttcacgcc
    11521 ttcgagtaga aagggcgcac gcccgcgccg atatcagcca aggtcccaca cggctgtcgg
    11581 aattgattct gccgggccgc actggcagga tgctgcgcct tccaactgct tgatctgatt
    11641 ccgaaatccc tatgcctacg attacgttgc cggacggcag ccaaaagaat ttcgaaacgc
    11701 ccgtgagcgt ggccgatgtc gcagcgagta ttggcgcggg cctggccagg gcggcgatcg
    11761 ccggccgggt caacgacaag ctggtc
//
LOCUS       DATDEU010000454         4880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1690146, whole genome shotgun sequence.
ACCESSION   DATDEU010000454 DATDEU010000000
VERSION     DATDEU010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690146"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /gene="uvrD"
                     /locus_tag="VIV63_10885"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             <1..1936
                     /gene="uvrD"
                     /locus_tag="VIV63_10885"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEY6125148.1"
                     /translation="GIPGSSMWVGTFHGIAHRLLRLHWRDAGLPQTFQILDGEDQLRL
                     IKKIVKALELDEARWVPREIQYFINHNKDEGHRPATLKDGSDPTRRQLIKLYTEYEAQ
                     CQRAGVVDFAELLLRAYELWLKNPQILRHYQQRFRHVLIDEFQDTNAIQYAWSLLVAG
                     QDGAPFVVGDDDQSIYRWRGARVENLTRFSKDYPAAKLYRLEQNYRSTGNILKAANAL
                     ISNNTGRLGKNLWTEGKDGERIKLYAAFNERDEAEFVMHRIRDWTNQGGARRDVAILY
                     RSNAQSRVFEEIFMSARIPYKVYGGLRFFERAEIKDALAYLRVITNHADDASFERVVN
                     LPTRGIGNKSLDVVRDQAKAATTSLWEAAAHCVANGVLGPKAEAAVNGFLKLVEQLGR
                     DIDGLELHEQVDHVINNSGLVEHHKKEKADRGEARVENLNELVSAARGFEPDGLNTDD
                     EALPPLAAFLSHAVLESGEGQAEAWEDCIQMMTLHTAKGLEFPVVFLCGLEDGLFPHQ
                     RSLNDEEGLEEERRLCYVGTTRAMKHLYMTYAEQRRLHGQDNYGTPSRFISEIPEDLI
                     EEVRPRIQISRPVAAGRFKPPTEELAPGVKLGARVRHKKFGEGVILKVEGNGPQANIQ
                     VNFSSLGVKIMMLEYLEVVR"
     gene            1933..2748
                     /gene="pyrF"
                     /locus_tag="VIV63_10890"
     CDS             1933..2748
                     /gene="pyrF"
                     /locus_tag="VIV63_10890"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY6125149.1"
                     /translation="MISFAERLNAAWKATNSLVCVGLDPEPKKFPDQFRDAPGGIFQF
                     NRAIIDATRDLVCAYKPQFAHYAAHAAENQLEHTIDYIKSTCPNAVVILDSKRGDIGS
                     TAEQYAHEAFERYDADAVTVNPYLGRDSVEPFLKHGDKGVIILCRTSNPGAKDFQDLD
                     VGGKKLFQHVAETVAKEWNTANNCMLVVGATYPEELADIRRRVGDLPFLVPGVGAQGG
                     DVAKVMAAGKTKAGTGLVISSSRAILYASSGEDFATAARKAAQDLRDSINAHR"
     gene            complement(2827..3150)
                     /locus_tag="VIV63_10895"
     CDS             complement(2827..3150)
                     /locus_tag="VIV63_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125150.1"
                     /translation="MSAPPAKALPAKPLDLRIGNVTRYMMPSEYRAALGAPDMEKNTV
                     VVEAKRELLPVEYEEPIPTWPLGPLWWGLKNPSQSWRIFLPDINAPEDGPPDVVPPPI
                     FRWGP"
     gene            complement(3396..>4880)
                     /locus_tag="VIV63_10900"
     CDS             complement(3396..>4880)
                     /locus_tag="VIV63_10900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY6125151.1"
                     /translation="KTFPRAFLNTPREFKVHYSTGQPARSDQPLNNTFGLSGEGKLVV
                     DGDAMILEGNRSGFTLAGPPRIALADVANVDYNAQSRAFLIRTRDGRHYVIVWLQTSE
                     DAEALWAMLPQEKTPEFLEDQAAHERFGKAMKLLGGRSPVTPAIIAINVAVFIVMFLA
                     GADLLNPSSDVHIRFGSNFGPLTWTGQEWRLLTSAFLHFGIIHIALNMFALYQGGALA
                     ERLFGSTRFAVIYLLSALSGSVASGWWDPLRNSAGASGAIFGVYGALLAFMAIRRSDI
                     PPSMFKSISSSALLFCLYSLVIGAAHPLIDNACHIGGLLAGFASGVILARPFTPEARS
                     TPQPAKVLVAILAIVLPLALMAQPLITGSNSKAATLRFERALEEFGRVEAELVRKQSE
                     ILTFPANVRVNRLEVAKRLRTEVLEPWRVATRPLLQSNTLEHDGSRATQRQEYIRDYL
                     QARERAVERQAIALETGDPEDEALAVAANAHVGETLKRLTALGK"
BASE COUNT          946 a         1432 c         1649 g          853 t
ORIGIN      
        1 tggcatccca ggaagcagca tgtgggtggg cacattccac ggcatcgcgc accggctgct
       61 gcgcctgcac tggcgcgacg cgggactgcc gcagaccttc cagatcctcg acggtgaaga
      121 ccagctccgg ctcatcaaga aaatcgtgaa ggccctggag ctcgacgagg cgcgctgggt
      181 gccgcgcgag atccagtact tcatcaatca caataaagat gaagggcatc ggccggcgac
      241 gctgaaggac ggtagcgatc cgacgcgccg gcagctcatc aagctgtaca cggaatacga
      301 agcgcagtgt cagcgcgcgg gcgtcgtgga tttcgcggag ctgttgctgc gcgcatatga
      361 gctgtggctc aagaatccgc agatcctgcg gcactatcag cagcgtttcc gtcacgtgct
      421 catcgacgag ttccaggaca cgaacgccat ccagtacgcg tggtcgctgc tggtggcggg
      481 acaggatggc gcgccgttcg tcgtcggcga cgacgaccag tccatctacc gctggcgcgg
      541 cgcgcgcgtg gagaacctca cgcgcttctc caaggactat ccggccgcga agctttatcg
      601 cctggagcag aactaccgct ccacgggcaa catcctgaag gcggcgaacg cgctgatctc
      661 caacaacacg gggcgcctcg gcaagaacct gtggacggaa ggcaaggacg gcgaacgcat
      721 caagctgtac gcggcgttca acgagcgtga cgaagctgaa ttcgtgatgc atcgcatccg
      781 cgactggacc aaccagggcg gcgctcggcg cgacgtggcg attctctatc gatcgaacgc
      841 gcagtcgcgc gtgttcgaag aaatcttcat gtcggcgcgc attccgtaca aggtgtacgg
      901 cggtctgcgg ttcttcgagc gcgcggaaat caaggatgcg ctggcgtacc tgcgcgtgat
      961 caccaatcac gccgacgacg cgtcgttcga gcgcgtggtg aatctgccga cgcgtggcat
     1021 cggcaacaag tcgctggacg tggtgcgcga tcaggcgaaa gccgcgacga cgtcgctatg
     1081 ggaagcggcg gcgcattgcg tggcgaacgg cgtgctgggg ccgaaggccg aagccgcggt
     1141 gaacggcttc ctgaagctgg tcgagcagtt gggccgcgat atcgatggtc tcgagctgca
     1201 cgagcaggtc gaccacgtga tcaacaactc agggttggtc gagcaccata agaaagagaa
     1261 ggccgatcgc ggcgaagcgc gcgtcgagaa cctgaacgaa ttggtgtcgg ccgcgcgtgg
     1321 cttcgagccc gatggcctga acacggacga cgaggcactg ccgccgctgg ctgcgttcct
     1381 ctcgcacgcg gtactggaat ccggcgaagg ccaggccgaa gcgtgggaag actgcatcca
     1441 gatgatgacg ctgcacacgg cgaagggtct cgagttcccg gtggtgttcc tgtgcggcct
     1501 cgaagacggc ttgttcccgc atcagcgctc gttgaacgac gaggaagggc tggaggaaga
     1561 gcgccggctt tgctacgtgg gcacgacccg cgcgatgaaa cacttatata tgacgtacgc
     1621 cgagcagcgg cgtctgcacg gccaggacaa ctacggcacg ccgtcgcggt tcatctcgga
     1681 gattcccgag gacctgatcg aagaggtgcg gccgcgcata cagatttcgc ggccggtggc
     1741 cgcgggccgg ttcaagccgc cgacggaaga gctggcaccg ggcgtgaagc tgggcgcgcg
     1801 agtgcggcac aagaagttcg gcgaaggcgt gatcctgaaa gtggaaggca atggcccgca
     1861 ggcgaacatc caggtgaatt tctcgtcgct gggcgtcaag atcatgatgc tggaatatct
     1921 cgaggtggtt aggtgatcag cttcgcggaa agactgaacg cggcgtggaa ggcgacgaat
     1981 tcgctggtat gcgtggggct cgatccggag ccgaagaagt ttccggacca gttccgcgat
     2041 gcgccgggcg gcatcttcca gttcaatcgc gccatcatcg acgcgacgcg cgaccttgtg
     2101 tgcgcgtaca agccgcagtt cgcgcattac gcggcgcatg cggcggagaa ccagctcgag
     2161 cacaccatcg actacatcaa gtcgacgtgt ccgaatgcgg tggtgatcct ggattcgaag
     2221 cgcggtgaca tcggcagcac ggccgagcaa tacgcgcatg aggcattcga gcgttacgac
     2281 gccgacgcgg tgacggtgaa tccctacctc ggtcgcgatt cggtcgagcc attcctcaaa
     2341 cacggcgaca agggcgtgat cattctctgc cgcacgtcga atccgggcgc gaaggatttt
     2401 caggacctgg acgtgggcgg caagaagctg ttccagcacg tcgccgagac ggtggcgaag
     2461 gaatggaaca cggctaacaa ctgcatgctg gtggtgggcg cgacgtatcc cgaagagctc
     2521 gcggacatcc gccggcgcgt gggcgatctg ccgttcctcg tgccgggcgt cggcgcgcag
     2581 ggtggcgacg tggcgaaggt gatggcggcg ggtaaaacca aggccggtac ggggctggtg
     2641 atcagctcgt cacgcgcgat tctctatgcc agctcgggcg aggacttcgc gactgcggcg
     2701 cgcaaggctg cgcaggatct gcgcgactcc atcaacgcgc atcggtaaaa ggggacagat
     2761 ctatttattt agctggccgt cagcgctgct cgcctggcgt ggaaaataaa tagatctgtc
     2821 ccctttttac gggccccagc ggaagatagg cggcggcacc acgtcgggcg ggccatcttc
     2881 cggtgcgttg atgtcgggca aaaagatgcg ccagctctgt gagggattct tgagacccca
     2941 ccacagtgga cccagcggcc acgtgggaat gggctcttca tattccaccg gcagcaactc
     3001 gcgcttggct tcgaccacca cggtgttctt ttccatgtcc ggcgcaccga gcgcggcgcg
     3061 gtattcactc ggcatcatgt agcgagtgac gttgccgatt cgcaagtcga gtggtttggc
     3121 cggcaaagcc ttggcaggag gcgcggacac gggtgcaacc gaaggcgcag gctcggttgc
     3181 aggtgcaggt gcggtcgtag tgacgggaat gggagccgcg gctgctggaa cggccgcagc
     3241 cggctctgcg gcatcggccg ctgaaaacat gagaagcgtc agacccaagc ccaagctgcg
     3301 tttcatgacc tcgttccctg tttgaggcgc gtccccattt gctagcaaat gaggaatgtc
     3361 cccatttccg accctgcgtt acgaggttag gttcctcact tgcccagggc ggtgaggcgt
     3421 ttcaaggttt cgccaacgtg ggcattcgcg gccaccgcca acgcctcgtc ctccgggtca
     3481 ccggtctcga gggcgatcgc ctgccgttcg acggcgcgct cacgggcctg cagataatcg
     3541 cgaatgtatt cctgtctttg ggtggcacgc gaaccgtcgt gctccaacgt gttggactgc
     3601 aacaaaggcc gcgtggcgac acgccagggt tcgagcactt cggtgcgcag gcgtttggcg
     3661 acctcgagcc ggttcacgcg cacgttcgcc ggaaaagtga ggatttcgct ctgtttgcgc
     3721 acgagctcgg cttcgacccg gccaaattct tcgagtgcgc gttcgaagcg aagagtcgcc
     3781 gccttgctgt tgcttcccgt gatcagcggc tgggccatca aggccagcgg cagcacgatg
     3841 gccaggatgg cgaccaggac cttcgcgggt tgcggtgtcg aacgcgcctc gggcgtgaag
     3901 ggacgcgcga gaatcacgcc ggacgcgaag ccggctaaca acccgccgat gtggcacgcg
     3961 ttgtcgatga gtggatgggc ggcaccgatc accagtgagt acaggcagaa gagcagcgcg
     4021 ctgctgctga tgctcttgaa catcgagggt ggaatgtcgg agcggcgtat ggccatgaag
     4081 gcgagcagcg cgccatacac gccgaagatc gcgcccgaag ctccggcgct gttgcgcaac
     4141 ggatcccacc atccactcgc gacgctgccc gagagcgcgg agagaagata gatcacggcg
     4201 aaacgggtgc tgccgaacag ccgttcggcc agcgcaccgc cctggtacag cgcgaacatg
     4261 ttcagcgcga tgtggatgat gccgaaatgc aggaaggccg aggtgagcag gcgccactcc
     4321 tggcccgtcc aggtcagcgg accgaagttg gagccaaagc ggatgtgtac gtcggagctg
     4381 ggattcagga gatccgctcc cgcgaggaac atgacgatga acaccgcgac gttgatcgcg
     4441 atgatcgcgg gcgtgaccgg cgatcgcccg ccgagcagtt tcatcgcctt gccaaaacgt
     4501 tcgtgcgccg cctggtcttc gaggaactcc ggcgtctttt cctgtggaag catcgcccac
     4561 aaagcttcgg cgtcttcgct tgtctgcagc cacacgatca cgtagtggcg gccgtcgcgc
     4621 gtacggatca ggaatgcacg gctctgggcg ttgtagtcga cgttggcgac gtcggcgagc
     4681 gcgatgcgcg gcggtccggc cagcgtgaag ccgctgcggt tgccttcgag aatcatcgcg
     4741 tcgccgtcga cgaccagttt gccctctccc gagagcccga acgtgttgtt gagcggctgg
     4801 tcggaccgtg ccggctggcc ggtgctgtaa tgcactttga attcgcgcgg ggtgttcaaa
     4861 aaggctctcg gaaacgtttt
//
LOCUS       DATDEU010000455         6468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2128041, whole genome shotgun sequence.
ACCESSION   DATDEU010000455 DATDEU010000000
VERSION     DATDEU010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128041"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..849
                     /locus_tag="VIV63_10905"
     CDS             91..849
                     /locus_tag="VIV63_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125152.1"
                     /translation="MKAVMRELAQAKRHYSRLPLFEFLRADSIPPRDRLAFYPCMAPF
                     ILAFSDLNRFVLRDEDSKDPHQLLINEHTYEDDHHWPWYLEDFTKLGFDRTASVTQTL
                     RSYMKDDTRQNRMLGSRLAQLIHGITPTEKLVVVEAIEETGNVLFGLTSKIASRIQAD
                     GGPELLYLGQFHFSRETGHAMHGADHRVLEAISLTELERVRCLDLAFRVFEMFTDWTG
                     ELLAYAKNSLAHRTVPHVIHTSIARQAAQQGVAP"
     gene            846..3767
                     /locus_tag="VIV63_10910"
     CDS             846..3767
                     /locus_tag="VIV63_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6125153.1"
                     /translation="MNLRKIQALAAMTGATALAGSLTPAIAQDSNGQTAQPEILETVT
                     VTGSRIRRDDYVSDSPTVTVSAEAMSETGSTAMEHLLNSLPQFVPSITTTSNNPGNGG
                     QANIDLRGLGTQRNLVLLNGRRLPPSNPTGTVDVNIIPSALIENIEVVTGGASAVYGS
                     DAIAGVTNFTLKRNFEGVAIDTGFGRTAQSDGQEWSSSLTLGSNFSEDRGNAVFSFQY
                     TERDAIYQGAREFSSITLDVRRQGNEPLGSSFIREGRFGRDDENSFTQAAMNSVFGRY
                     GAAPGSVPFSQHIGFNADGTLFTMGTGQPGSVANFRGDTTDPSFNDAGYSYNFAPPNA
                     LTIPVKRWNLAGFANLALSDRTEAYVQTFFTTYDTQLTLAPVPANDLTIPVTNPFIGA
                     DLAELLASRPDPDAPMDFRQRMEGVGPRESQDKYDVYQLLAGLRGKLGERFDWDMYVS
                     SSSMSDTNVLNNDISGARLQELLNSPTGGEDKCAGGYDPFGGPAGLSPECADYVRAYF
                     TNRTQLDSSIAEATIGGKAFSLPAGDAKFSVGASWREETYDFRPDLAVARGDLLGFTR
                     QFPLKGGFHVTDVFGELYLPVLADKRFAQDLGFTLGARLSDHSLSGSANAFKVEGSWQ
                     PVDLMRVRASYQRAVRAPSIAELFSPENENFPTLLEDPCDNTSSARRFGANADAAHGG
                     NSSIRSLCIEQGISAASIDTYRFGNGQVRTTGGGNPDLREEQADTVTLGVVFSFEKVR
                     ASVDFYSIKLNDAIFSIPAGEIVLLCYGFSGNNPDLRADDPACQAIDRLSQSSREPVV
                     PSQGTDNVSSLRTAGVDVQVDWGVGLGRAGQIDLNLLGNWLQKWEISYLPGIPVIDYR
                     GTIGDNVGGAFPDYKLLLNARWTLADFGAGIRVQHLPAMTNKYASYDPFTTVDVPAIT
                     YLDANVSWRHDEQFEVRLGVENLTDETPPLYTAGVQMNTDPSTYDVLGRRYYLRANMK
                     F"
     gene            3770..4669
                     /locus_tag="VIV63_10915"
     CDS             3770..4669
                     /locus_tag="VIV63_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY6125154.1"
                     /translation="MQGTHTRAANVPHLGLVLGSGGVRSVAALGIADVLVNAGLRPDL
                     VVGCSSGALFGACIAMGMTGEQGLAAAMRLWSVELTQQKRWRAYLELLAPRIMGFDAG
                     FSLRDARLIGARVERAFGDAQLQDLAILMRVVATDAATGDSVVLSRGRVTDAVRASMA
                     LPFIFPSVEIDGRRLSDGVISDPLPVAAARDADAVITLGFRGAMPRRVDRPARLIGQV
                     TTAMINNLQQARIDAARAAGQRMLHLEVDIDRRTGLWDTAAMPRIFEKGQRAAADALP
                     AIRRLLDAPRRSPPTPYAAVSNQ"
     gene            4666..5127
                     /locus_tag="VIV63_10920"
     CDS             4666..5127
                     /locus_tag="VIV63_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6125155.1"
                     /translation="MKTNRRLLVPIALAAALVTSVSAAKDDPAAIVAALDTEYQAAVE
                     RNDWQVMDRILHPDFTLVLGNGKVYSRAELIESARDRHIEYEVQVEMPDTQTVRVFGN
                     DTATVTALLWLKGTRKSDASTFEYKLWFSDTYVRTKDGWRYAFGQASLRLP"
     gene            complement(5223..6026)
                     /locus_tag="VIV63_10925"
     CDS             complement(5223..6026)
                     /locus_tag="VIV63_10925"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6125156.1"
                     /translation="MTGIAKLKVILTGASEGIGRALALALAAQGARLALAARDRERLE
                     SLAQECRALGGEARALPTDVTNEQDCEWLVAETVKAFGGIDVVIHNAGITMWSRFDAL
                     QDLSIFERVMEVNYLAPVRLTALSLPHLKQSKGLLVAVASLAGLTGVPERSAYAASKH
                     AMIGFFDSLRIELRGSGVDVSVVAPDFVVSEIHKRAIGPDGEPLGASPMMQAKIMTAE
                     ECAKRIVRAIGKRERLVLMSARGRLGRWLKLLAPSLIDRIAERAIRQRH"
     gene            6090..>6468
                     /locus_tag="VIV63_10930"
     CDS             6090..>6468
                     /locus_tag="VIV63_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125157.1"
                     /translation="MSWLLRRFLKLWVRAAVQPADAPSSLTDPRGPVCYVLESDSMAD
                     RAVLCNVATRLGLPYPEKRSSSLPVEERRTYFDVARRRRFWDATQTRKPPPYLLALVE
                     ALSADSVRDILLVPTAVYWGRAPQ"
BASE COUNT         1116 a         2209 c         2049 g         1094 t
ORIGIN      
        1 tggggccctc ttatcctcca tctcctctgt ccgctttccc cccaagcccg cgtcagtact
       61 ccatgtgacc acccatgtcc tggagaaatg atgaaggccg tgatgcgaga gctggcgcaa
      121 gccaagcgac actattccag actgccgctg ttcgaattcc tgcgcgcaga ctcaatcccg
      181 ccgcgcgacc gcctggcgtt ctacccgtgc atggcgccat tcatcctggc gttctcggat
      241 ctcaatcgct tcgtgctgcg cgatgaggat tcgaaagatc cgcatcagct gctgatcaac
      301 gaacacacct acgaggacga ccatcactgg ccgtggtacc tcgaggattt caccaagctc
      361 ggcttcgatc gcaccgccag cgtcacccag acgctgcggt cgtacatgaa ggacgacacg
      421 cggcagaacc gcatgctggg ctcgcggctg gcgcaactga tccacggcat cacgcccacg
      481 gagaagctcg tcgtggtcga ggcgatcgag gaaaccggca acgtgctgtt cggcctgacc
      541 tcgaagatcg cctcgcgcat ccaggccgac ggtggccccg aactgctgta cctcggtcag
      601 ttccacttca gccgcgagac gggacacgcc atgcacggcg ccgaccatcg cgtgctcgaa
      661 gccatctcgc tcaccgagct agaacgcgtg cgctgcctcg atctcgcctt ccgcgtcttc
      721 gagatgttca ccgactggac cggcgaacta ctggcctacg ccaagaactc gctcgcgcac
      781 cgcaccgtgc cgcatgtcat ccacacgagc attgcgcgcc aggccgcgca gcagggagta
      841 gctccatgaa tctccgcaag atccaggccc tggccgcgat gaccggcgcg acggcgctcg
      901 ccggtagttt gacgccggcg atcgcgcagg attcgaacgg gcagacggcc cagcccgaaa
      961 tcctcgagac cgtcaccgtg acgggctcgc gcatccgtcg cgacgattac gtgtccgaca
     1021 gcccgaccgt gacggtgagc gccgaggcca tgtccgaaac cggctcgacg gcgatggagc
     1081 acctgctcaa ctcgctgccg cagttcgtgc cgtcgatcac gacgacgtcc aacaatccgg
     1141 gcaatggcgg ccaggccaac atcgacctgc gcggtctcgg cactcaacgc aacctggtgt
     1201 tgctcaacgg ccggcggctg ccgccgtcga atcccaccgg caccgtcgac gtgaacatca
     1261 ttccttccgc gctcatcgag aacatcgaag tggtcacggg cggcgcgtcg gccgtgtacg
     1321 gctccgacgc catcgccggc gtcacgaatt tcacgctgaa acggaatttc gaaggcgtgg
     1381 ccatcgacac gggcttcggg cgcacggcgc agagcgacgg ccaggaatgg tccagctcgc
     1441 tcacgctggg ctccaatttt tccgaagacc gcggcaacgc cgtgttcagc ttccagtaca
     1501 ccgagcgcga cgccatctac cagggcgcgc gcgaattctc gagcatcaca ctcgacgtgc
     1561 gccgccaggg gaacgagcct ttgggctcga gcttcatccg cgaaggacgc ttcggccgcg
     1621 acgacgaaaa ttccttcacg caggccgcga tgaattcggt tttcggccga tacggcgcgg
     1681 cgcccggcag cgtgccgttc tcgcagcaca tcggcttcaa cgccgacggt acgttgttca
     1741 ccatgggcac gggtcagccc ggcagcgtcg ccaattttcg cggcgacacc accgatcctt
     1801 cgttcaacga cgcgggctac agctacaact tcgcgccgcc caatgcgctg acgataccgg
     1861 tgaaacgctg gaacctcgcg ggcttcgcca acctggcgct cagcgacagg acggaagcgt
     1921 acgtgcagac tttcttcacg acgtacgaca cgcagctgac gctggcgccg gtgccggcga
     1981 acgacctcac gattccggtc accaacccct tcatcggcgc agaccttgcc gagctgctgg
     2041 cgtcgcgccc ggatcccgac gcacccatgg acttccgcca gcgcatggag ggtgtcgggc
     2101 cgcgcgagag ccaggacaag tacgacgtct accagctgct ggccggcctg cgcgggaagt
     2161 tgggcgaacg cttcgactgg gacatgtatg tgtccagttc gagcatgtcc gacaccaacg
     2221 tcctcaacaa cgacatctcc ggcgcgcgac tccaggaact gctcaactcg ccgaccggtg
     2281 gcgaggacaa gtgcgcgggc gggtatgacc cgttcggcgg cccggcgggt ctttcgcccg
     2341 agtgtgccga ctacgtgcgc gcctacttca ccaaccggac ccagctcgac agctccatcg
     2401 ccgaagcgac gatcggtggc aaggcgttct cgctgccggc cggtgatgcc aagttctcgg
     2461 tcggcgcgag ctggcgcgag gagacctacg atttccggcc ggaccttgcc gtggcgcgcg
     2521 gcgatctgct cggtttcacc cggcagtttc cgctcaaggg cggctttcac gtgaccgacg
     2581 tgttcggcga gctctatcta cccgtgctcg cggacaagcg gttcgcccag gacctgggct
     2641 tcacgctcgg cgcgcgcctc tcggaccact cgttgtccgg cagcgccaac gcgttcaagg
     2701 tggagggcag ctggcagccg gtcgacctga tgcgtgtgcg tgcctcatac cagcgcgcgg
     2761 tgcgcgcgcc ttcgatcgcc gaattgttca gcccggagaa cgagaatttc ccgacgctgc
     2821 tcgaggaccc ttgcgacaac acgtcgtcgg cgcggcgatt cggcgcgaat gccgacgcag
     2881 cgcacggcgg aaacagctcc atccgcagcc tgtgcatcga acagggcatt tccgcggcca
     2941 gcatcgacac ctaccgattc ggcaacggac aggtgcgcac gacgggtggt ggcaatcccg
     3001 acctcaggga agagcaggct gacaccgtca cgctgggagt ggtcttcagc ttcgagaagg
     3061 tgcgcgcgtc tgtcgatttc tattcgatca agctgaacga cgcgatcttc tcgattccgg
     3121 ccggtgaaat cgtgctgctg tgttacggct tctcgggcaa caacccggac ctgcgagcgg
     3181 atgatccggc ctgccaggct atcgaccgcc tgtcgcaaag cagccgcgaa cccgtcgtcc
     3241 ccagccaggg caccgacaac gtgtcgtcgc tgcgcaccgc gggcgtcgac gtgcaggtgg
     3301 actggggcgt cggcctgggt cgcgcgggcc agatcgatct caacctgctc ggcaactggc
     3361 tgcagaaatg ggagatctcc tatctacctg gcatcccggt catcgactat cgcggcacca
     3421 tcggcgacaa cgtcggcggc gcattcccgg actacaagct gctgttgaac gcgcgctgga
     3481 cgctcgccga tttcggggcc ggcatacgcg tgcagcacct gccggcgatg acgaacaagt
     3541 acgcgtcgta cgatccgttc accacggtgg atgtaccggc gatcacctat ctagacgcca
     3601 acgtgtcctg gcgccacgac gaacagttcg aagtccggct cggcgtcgag aatctgaccg
     3661 acgagacgcc gccgctgtac accgccggtg tgcagatgaa taccgatccg tccacctatg
     3721 acgttctcgg gcgccgctac tacctgcgcg ccaatatgaa gttctgatca tgcagggcac
     3781 tcacacacgc gcggcgaatg tgccgcacct gggcctggtt ctcggcagcg gcggcgttcg
     3841 cagcgtcgcc gcgctcggaa ttgccgacgt cctcgtgaat gcggggctgc ggcccgacct
     3901 cgtcgtgggc tgcagctcgg gcgcgttgtt cggcgcatgc atcgcgatgg gcatgacggg
     3961 cgagcagggc ctcgcggcgg cgatgcggct gtggtcggtg gaactcacgc agcagaaacg
     4021 ctggcgcgcc tacctcgagc tgctggcgcc gcgcatcatg ggcttcgacg cgggcttctc
     4081 gctgcgcgac gcacgcctga tcggcgcgcg cgtggaacgc gcgttcgggg atgcgcagct
     4141 ccaggatctc gcgatcctga tgcgcgtcgt ggcgacggat gccgccaccg gcgattcggt
     4201 ggtcttgagc cgcgggcgcg tgacggacgc cgtgcgcgcc agcatggcgt tgccgttcat
     4261 cttcccgagc gtggagatag acgggcggcg actcagcgac ggcgtgatct ccgatccgct
     4321 gccggtagcg gccgcgcgcg atgccgacgc cgtcatcaca ctggggtttc gtggcgcgat
     4381 gccgcgtcgt gtcgatcggc cggcgcgcct catcgggcag gtcacgacgg cgatgatcaa
     4441 caatcttcag caggcgcgga tcgacgccgc gcgggccgcg ggtcagcgca tgttgcacct
     4501 ggaggtcgac atcgaccgcc gcaccggatt gtgggatacc gccgccatgc cacgcatttt
     4561 cgagaaaggg cagcgcgccg cggccgatgc gttgccggcc atccggcggc tgctcgatgc
     4621 gccacgcaga tcgccgccca ctccttacgc cgcagtgagc aaccaatgaa gacgaatcga
     4681 cgactcctcg ttccgatcgc gttggccgcg gcgctcgtca ccagcgtttc ggcggcaaaa
     4741 gacgatcccg cggcgatcgt tgccgcgctc gacaccgagt accaggctgc cgtcgagcgc
     4801 aacgactggc aggtgatgga tcgcatcctg catcccgatt tcacgctggt gctcggcaac
     4861 ggcaaggtgt actcgcgcgc cgagctcatc gagtccgcgc gcgaccggca catcgagtac
     4921 gaggtgcagg tggagatgcc ggacacgcaa accgtgcggg tcttcggcaa cgacacggcc
     4981 accgtgaccg cgctgttgtg gctcaagggc acgcgcaaga gcgacgcgag cacgttcgaa
     5041 tacaagttgt ggttcagcga tacctacgta cgcacgaagg acggctggcg ctacgcattc
     5101 ggccaggcgt cgctacgcct gccgtaatgg ggacattcct gatttgctgg caaaaaggga
     5161 cgcggctcac tgaagaggcg cgtccccata tgctgcttat caggaatgtc cccattaacc
     5221 catcaatgcc gctggcggat ggcgcgctcg gcgatgcggt cgatcagcga cggcgcgagt
     5281 agtttgagcc accggccgag gcggccgcgc gccgacatca acaccagccg ctcccgcttg
     5341 ccgatcgcgc gcacgatgcg cttcgcgcat tcctcggcgg tcatgatctt cgcctgcatc
     5401 atcggcgacg cgcccagcgg ttcgccatcc ggcccgatcg cgcgcttgtg gatctcgctg
     5461 acgacgaaat ccggcgcgac gacgctcaca tcgacgccgc tgccgcgcag ctcgatgcgc
     5521 agcgaatcga agaacccgat catcgcgtgt ttgctcgccg cataggctga gcgctcgggc
     5581 acgccggtca gtcccgcgag gctcgcgacc gccaccagca gtcccttcga ctgcttgaga
     5641 tgtggcaacg acagcgcggt gaggcgcacg ggcgcgaggt agttcacctc catgacccgc
     5701 tcgaagatcg acagatcctg gagcgcgtcg aaccggctcc acatggtgat gccggcgttg
     5761 tgaatgacca cgtcgatgcc gccaaacgct ttcacggttt cggcgaccag ccattcgcag
     5821 tcctgctcgt tcgtgacgtc ggtgggcagg gcgcgggctt cgccacccag tgcccggcat
     5881 tcctgtgcca gcgattcgag gcgctcccgg tcccgcgcgg ccagcgccag tcgggcgccc
     5941 tgcgcggcca gcgccagggc cagcgcacgc ccgattcctt cggaggcgcc cgtcagaatt
     6001 accttaagtt ttgcgatccc ggtcatgcct tacgctctcg gctacactcc gccgcctaac
     6061 atcatacggc gggtggcgct cgtaaacgca tgagctggtt gctgcgacga ttcctcaagc
     6121 tctgggtccg ggccgccgtg cagcccgccg acgcgccgtc gtcgttgacg gatccgcgcg
     6181 ggcccgtctg ttacgtgctc gagagcgatt cgatggccga tcgcgcggtg ctgtgcaatg
     6241 tcgccaccag gctcggcctg ccgtatcccg aaaagcgctc gagcagcctg cccgtggaag
     6301 agcggcgcac gtatttcgac gtggcccgcc ggcgccgttt ctgggatgcc acgcagacgc
     6361 gcaagccgcc gccgtatctg ctcgcgctgg tcgaagcgct gagcgccgac tcggtgcgcg
     6421 acatcctgct ggtgcccacg gcggtgtact ggggccgcgc gccgcaga
//
LOCUS       DATDEU010000456         3328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2128396, whole genome shotgun sequence.
ACCESSION   DATDEU010000456 DATDEU010000000
VERSION     DATDEU010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128396"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="VIV63_10935"
     CDS             <1..1774
                     /locus_tag="VIV63_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEY6125158.1"
                     /translation="TCGQDIFRGDRLPAELRGNLLFAEPVGRLVRRTLIKVEDGVTRL
                     ANAYEDQHSEFIRATDPLFRPVNMVTAPDGTLYLVDMYRGIIQQANWTRKGSYLREQI
                     EAHDLQKEIGRGRVYRLRHESFEPGPKPRMLDETPAEWVKHLSHPNGWWRDTAQKLLV
                     LRHDLSVVPALEQLASSDSTDSRPRLHALWTLEGLGALDAKLIQRALKSADPQVRIAG
                     LRLADVRVNAKSTAKAPLAVALRGALRDADPSVTIQAMLSVRRAGLPDAQKVIKATAE
                     ASTSAGVYAINEQLWDEANNEDPQLIRLLGVNGLKSYRAGSTLYNSLCFACHGTDGRG
                     APMPGAAGRTLAPSLANSPRVLGDERAIIAILLHGLQGAVDGVDYGAPMVPMNSYSDA
                     ELAGVLTYVRNSFGNRAPAVEAGAVAARRAADAGRANYWTLDELTGQLPALGVPRERF
                     KRRAEWKLAANMEARKSSPVEAMLDGDPKTGYFTEGFKPFPVEWLTVELPARSKIVGI
                     DIDSRGEKSDNGKDMGWAPFYSVEVSDDGKTWTMVNAKVVGEPHARFNLAAPVEARHL
                     RIAIVEKEGWQPWVIKELNLYGQE"
     gene            1779..2765
                     /locus_tag="VIV63_10940"
     CDS             1779..2765
                     /locus_tag="VIV63_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6125159.1"
                     /translation="MAQRRVRQTISLSLLTLLTLAGCSNGTGDYGAERRGPPVLGVSQ
                     VAAQANWDTANADSIRKAASDTGIELRLEDARRSQANQMAALRSFVRQHVDVIALSPV
                     VETGWEVVLREIRTAGIPVILMDRSIDISDHSLYVSMIGSDFVEEGRRAGRWLLENTR
                     DVPGEIGIVELQGTVDSAPANNRNQGFFEVIGPDRRYQLVRSESADFDRARARELMAE
                     FLKANGNRIRVVFAHSDTMALGAIEAIESAGFKPGSDILVIAIEGSRKGLEAIIAGKL
                     NVSVECSPLLGPQLMAVVKDLAADKPVPRRVITQESVFTRENAAAELPNRAY"
     gene            2863..>3328
                     /locus_tag="VIV63_10945"
     CDS             2863..>3328
                     /locus_tag="VIV63_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125160.1"
                     /translation="MKAAVLLTALTSSTGIDVSGDAREFAPEIISTRYSDVRLTISPD
                     GNTALWFSRNRPGGPGGYDIWMSRREATGWAEAVSVSFNTASREFDPAFSRDGRFVYF
                     ASDRAGGVGGDDLYRVAVTKHGFGAVEHLGNEVNSARNEWAPMMFGNTLLFSS"
BASE COUNT          615 a         1102 c         1045 g          566 t
ORIGIN      
        1 aacgtgtggc caggacattt tccgcggtga ccgactgccg gccgagttgc gcggcaatct
       61 gttgttcgcc gagcccgtcg gccgtctcgt ccgccgcacg ctgatcaagg tcgaggatgg
      121 tgtcacccgc ctggccaacg catacgagga tcaacacagc gaattcatcc gcgcaacgga
      181 tccgctgttc cgccccgtca acatggtgac cgcaccagac ggcacgctct atctagtgga
      241 catgtatcgc ggaatcattc agcaggccaa ctggacgcgc aaaggctcgt acctgcgtga
      301 gcagatcgaa gcccacgatc tgcaaaagga gattggccgg ggccgcgtct atcgcctgcg
      361 tcacgagagt ttcgagcccg gcccgaagcc gcgcatgctc gacgaaacgc cggccgagtg
      421 ggtgaagcac ctctcgcatc ccaatggctg gtggcgcgac accgcacaaa aacttctcgt
      481 tctacgccac gatctctcgg tcgtgccggc tctcgagcaa ctggccagca gcgactccac
      541 agattcccgc cctcgcctgc acgcgctgtg gacgctcgag ggcctcggcg ctctcgatgc
      601 caaactgatc cagcgcgcgc tgaaatccgc ggacccgcaa gtgcgcatcg cgggcctgcg
      661 gctggcggat gtacgcgtga acgccaaatc gactgccaag gctccgctcg cggtcgccct
      721 gcggggcgcg ctgcgtgacg ccgatccgag cgtcacgatc caggcgatgc tttccgtgcg
      781 ccgcgccggg ttgcccgacg cccagaaggt catcaaggcc actgccgaag cctcgacttc
      841 cgccggcgtc tacgccatca acgagcagct ctgggacgaa gccaacaacg aggaccccca
      901 gctcatccgc ctgctcggcg tgaacgggct caagtcctat cgcgcgggca gcactctcta
      961 caattcgctg tgcttcgcct gccacggcac cgacggtcgc ggcgcgccca tgccgggcgc
     1021 cgcaggccgc acgctcgcgc cgtcgttggc caactcgcca cgcgtgctcg gcgacgaacg
     1081 ggccatcatc gccatacttc tgcacggact gcagggagct gtggatggcg tcgactacgg
     1141 cgcgcccatg gtgccgatga actcctactc cgatgccgag ctcgccggcg tgctcacgta
     1201 cgtccgcaac agcttcggca accgggcgcc cgcggtcgag gccggcgccg tcgccgcgcg
     1261 ccgcgccgcc gatgccggcc gtgctaacta ctggacgctc gacgagctca ccggccagct
     1321 tcccgcgctc ggtgtaccgc gcgagcgctt caagcggcgc gccgagtgga aactcgccgc
     1381 caacatggag gcgcgcaaga gttcgcctgt cgaagcgatg ctcgatggcg atccgaagac
     1441 cggatatttc actgaaggat tcaaaccgtt ccccgtggag tggctcaccg tcgaactacc
     1501 tgcccgcagc aagatcgtcg gtatcgacat cgattcccgc ggtgagaaat ccgacaacgg
     1561 caaggatatg ggctgggcgc cgttctacag tgtcgaagtg tccgacgacg ggaagacctg
     1621 gaccatggtc aatgcgaagg tggtgggcga acctcacgcc cgtttcaacc tcgcggcgcc
     1681 cgtcgaagca cggcacctgc gcatcgccat cgtggaaaaa gaaggctggc agccctgggt
     1741 gatcaaggag ctcaacctgt acggccagga gtagttgggt ggcgcaacgc cgcgtccgac
     1801 agacgatttc gctctcgctg ctgacgctgc taacgcttgc gggctgctcg aatggcacgg
     1861 gcgactacgg cgcggagcgc agagggccgc cggtgctcgg cgtgagtcag gttgcggccc
     1921 aggccaactg ggacaccgcc aatgccgact ccattcgcaa ggcagcgagc gacaccggca
     1981 tcgaactgcg gctcgaagat gccagacgca gccaggcgaa ccagatggcg gcgttacgtt
     2041 ctttcgtccg ccagcacgtc gacgtgatcg ccttgtcacc cgtggtcgag accggctggg
     2101 aagtcgtgct gcgcgagatc cggacagcgg gaatccccgt gatcctcatg gatcgttcca
     2161 tcgatatcag cgaccactcg ttgtacgtgt cgatgatcgg ctccgacttc gtcgaggaag
     2221 gaaggcgagc cgggcgctgg ctgctcgaaa acacccgcga cgttccgggc gagatcggta
     2281 tcgtcgaact gcagggcacg gtggattccg cgcccgccaa caaccgcaat cagggcttct
     2341 tcgaagtcat cgggcccgac cgtcgctacc agctcgttcg ttcggagagc gccgacttcg
     2401 accgcgctcg cgcacgtgaa ctgatggcgg agttcctgaa ggcaaacgga aaccgcatcc
     2461 gcgtggtgtt cgcgcacagc gatacgatgg cgctgggcgc catcgaggcc atcgaatccg
     2521 cgggattcaa accgggaagc gacatcctgg tgattgccat cgaaggcagc cgcaaggggc
     2581 tcgaggccat catcgccggc aaactcaacg tgagtgtgga gtgcagtcct ctgctgggac
     2641 cgcagctcat ggccgtggtc aaggatctcg ccgccgacaa accggttccg cgtcgcgtga
     2701 ttacgcagga gtccgtcttc acgcgcgaga acgccgcggc ggaactgccc aaccgcgcgt
     2761 attgagcaaa gtcgccggta acccaaagtc tgccttgggc tacgggctgg tttactggct
     2821 ggtcgcggtg accctgccgc gtcgatgatc gccggaatga acatgaaggc tgcagttctt
     2881 ctcaccgcgc tcacatcttc gaccggcatc gatgtcagcg gcgacgcccg cgagttcgcg
     2941 cccgaaatca tctcgacccg ttactccgac gtgagactca ccatctcacc cgatggaaat
     3001 accgcgttgt ggttcagccg caatcgtccc ggcgggcccg gcggctacga catctggatg
     3061 tcacgccgcg aagccaccgg ctgggcagag gccgtctccg tgtcgttcaa tacggcttcc
     3121 cgcgagttcg atccggcatt ttcgcgcgat gggcgattcg tctacttcgc atcggatcgc
     3181 gcgggtggcg tcggtggcga tgatctttat cgcgtggccg tcacgaaaca cggcttcggc
     3241 gcggtcgaac acctgggcaa cgaagtgaac tctgcgcgca acgaatgggc gccgatgatg
     3301 ttcggcaaca ccttgctgtt ttcgagtg
//
LOCUS       DATDEU010000457         4776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1400003, whole genome shotgun sequence.
ACCESSION   DATDEU010000457 DATDEU010000000
VERSION     DATDEU010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400003"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..705)
                     /locus_tag="VIV63_10950"
     CDS             complement(187..705)
                     /locus_tag="VIV63_10950"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEY6125161.1"
                     /translation="MPRAHQGSAHAGHQDRQGPDLTFRDSFFAPPITAAASQPRQLPA
                     VETWFAAATAAGVRDFDYIGVSYYPKWSTFTMKQAGSALATIKKRFKADIIVVEVSYP
                     WTLRDAGDKASNLLGEDSLLRGYPATPTGQQRFMNDVVKMTREAGGVGVVYWEPAWVS
                     TSCATRWGTGSH"
     gene            complement(642..1343)
                     /locus_tag="VIV63_10955"
     CDS             complement(642..1343)
                     /locus_tag="VIV63_10955"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY6125162.1"
                     /translation="MSGSINLTQSLVQKLGSSIVRGDLPAGKPLPIEAELGKKFGASR
                     TVMREAVKILSTKGLIGQRPRVGTYVHPEEKWDLLDAEVLKWILDRHFSHKLVREFLE
                     VRIGIEPAAAALAATNATDEDKELLRQKLDRMKAANEGHFDPVSADIAFHATILEISH
                     NRFFHQLTPIIETALRFSIRLTNKVKGTLPDYDAHEKIYRAIRNGNPEAAFRSCRELI
                     KEALMLVIKTDKART"
     gene            complement(1491..2255)
                     /locus_tag="VIV63_10960"
     CDS             complement(1491..2255)
                     /locus_tag="VIV63_10960"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6125163.1"
                     /translation="MHTKTSSVKIGVQNIRKGFQNEKVNLQVIEGLDFEVREGEFVAI
                     VGPSGCGKTTLMNIMAGFLAPDSGVIMVDGKPRPQPNAKGVLITQQGSVFPWLTVRQN
                     LLFGLPDELPNREKLADEYAALVGLKGFENNYPHELSGGMLKRAEIARALVVKPEILY
                     MDEPFSALDALMSLRMQNELLRILSIERHTVLLITHDVEEAIHLADRILVLSPRPARI
                     QASFDVPLAHPRRVSSPEAQALRVAILRELGVDGTV"
     gene            complement(2245..3021)
                     /locus_tag="VIV63_10965"
     CDS             complement(2245..3021)
                     /locus_tag="VIV63_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6125164.1"
                     /translation="MGEQSYMKGLKQSLPSIIVIAVVVGIWWAIVIATQSAIFPTPWQ
                     VVTGTMELVEDGTLWEHIGASLMRVGAGFGIAVLFAIPLGLWMGWVRGAYVTLNPVFQ
                     ILRPISPIAWIPIAILWFGVGNASPIYLIFIASVFPMIVQTTAGVHTIEKRFLRAAEN
                     FGVSRRKLFLQVVFPATLPQIIVGMRIGLGVAWLVVVAAEMIALRSGLGYLIIDSRNA
                     GNRYDLVIAGMIIIGLIGLLLDGIMRMLESMKVVRWRYAH"
     gene            complement(3000..3431)
                     /locus_tag="VIV63_10970"
     CDS             complement(3000..3431)
                     /locus_tag="VIV63_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125165.1"
                     /translation="MSRTKRVLLAVPLLALLAALGWAGFAPVSVPSRDELFEIPKGTY
                     ARRMAGDKVEILPSKIRLTLGLNDVLLLRNLDDVPQVFGPTIMMPGQSFRLPFEKAAS
                     YDFACTAHASGQMTVLVEPEPVMPWAKIRWRVREWWENRAT"
     gene            complement(3428..4027)
                     /locus_tag="VIV63_10975"
     CDS             complement(3428..4027)
                     /locus_tag="VIV63_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY6125166.1"
                     /translation="MRPMPRWLQMMMVMLAGLATALANSPAPAGAMKAGVLSPVMAAP
                     ELKLQGTDGKPLTLSRYRGKVVLLAFGFSSCGEVCPITLATLAGARKKLGEAAANVQV
                     VYVTVDPERDDAARMKKYLASFDASFVGGVGSRADIDAAQKSYGISSTKQVYADGNYT
                     IGHSSSIYMIDRTGGLKAVMPYGHTVDDFVHDLKILLRP"
     gene            complement(4027..>4776)
                     /locus_tag="VIV63_10980"
     CDS             complement(4027..>4776)
                     /locus_tag="VIV63_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6125167.1"
                     /translation="AYMLAPLVMDLADKKIPVKIVSLGHRSGAVIMVRQDSAYQKFAD
                     LRGKRIAIPSRFAVDFLFLRKMLTRENMTPKDIEIVEMPPPDMPAALYANAVDAYCTG
                     EPFGAAAQRAGYARVLRMTRDEWRNYICCCLTVREELIAENPAMVQDLVNQVQSAGEW
                     LDQKQENRDKAVAIAAGKGFFNQDPNILKFVMENPTDRVTYGDLRMIREEFEDLMQLS
                     IAAGTIKHPIAYETYIDERFVRAQKPALIPL"
BASE COUNT          895 a         1495 c         1477 g          909 t
ORIGIN      
        1 atcgactcga gatccggaaa gttggccacc ccatccaccg tggcgttacg gctcgcgcaa
       61 gccgcaagct cgaagcgcga actcgcgcgc aacgatggga tatgctgatc gcgcgcgatc
      121 ttgccgatgc ctaacaatcc aactttcagc cgcatgtcgt ctccaggtgc cgaatgacgc
      181 attttttcag tgcgatcccg ttccccagcg cgtggcgcaa ctcgtggaaa cccaggctgg
      241 ttcccaataa acgacgccca ctccgccggc ctcgcgggtc atcttcacga catcgttcat
      301 gaagcgttgt tgacccgtgg gcgtcgcagg ataaccgcgc agcagcgagt cttcacccag
      361 cagattcgag gccttgtctc ccgcgtcgcg cagcgtccag ggatacgaaa cttcgacgac
      421 aatgatgtca gccttgaatc gcttcttgat cgtggcgagc gccgagcctg cctgtttcat
      481 ggtgaacgtg gaccacttcg ggtagtagct gacgccgatg tagtcgaaat cgcgcacgcc
      541 ggcggccgtc gcggctgcga accaggtttc gacagcgggg agttgccgtg gctgcgatgc
      601 cgctgcggtt atcggcggcg caaaaaagga atcgcgaaag gttaggtccg ggccttgtcg
      661 gtcttgatga ccagcatgag cgcttccttg atgagctcgc ggcacgagcg gaaagcggct
      721 tccggattgc cgttgcggat ggcgcggtag attttttcgt gcgcgtcgta atcgggcaac
      781 gtgcccttga ccttgttagt caggcgaatg gaaaaacgca acgcggtttc gatgatgggt
      841 gtgagctggt gaaagaaacg gttgtggctg atctcgagga tggtggcgtg aaacgcgatg
      901 tcggccgaca ccgggtcgaa atgcccctcg ttcgccgcct tcatccggtc gagcttctgc
      961 cgcagcagtt ccttgtcttc gtcggtggca ttggtggccg cgagcgccgc ggccgccggt
     1021 tcgatgccga tacggacttc gaggaactca cgcaccagct tgtgggaaaa atgccggtcg
     1081 aggatccact tcaggacctc ggcatccagc aggtcccact tttcttccgg atggacgtac
     1141 gtgccgacgc gcgggcgctg gccgatgagt cccttggtcg agaggatctt caccgcctcg
     1201 cgcatcacgg tacggctcgc gccgaacttc ttgccgagct ccgcctcgat gggcagcggc
     1261 tttcccgcgg gcagatcgcc gcgcacgatg gacgacccga gcttctgcac gagcgactgg
     1321 gtgagattga tgcttccgga catgcttcgt acgctatcac gcgcatgcga aatatgcgga
     1381 gtatcaatca gattgattgc cggcggggaa tccggccggc cgggacaggc gatcgtcccg
     1441 ccaggacacg gtggcgcacc ggctctgacg gcgcgccacc ggtctggtag ttagacggtc
     1501 ccgtcgacgc cgagctcccg gaggatcgcc acgcgcagcg cctgcgcctc cgggctcgac
     1561 acccggcgag ggtgcgcgag cggaacgtcg aacgatgcct ggatacgcgc cgggcgcggg
     1621 ctcagcacca ggatgcgatc cgcgaggtga atggcttctt cgacgtcgtg cgtgatcagc
     1681 agcaccgtgt gacgttcgat ggaaaggatg cgcagcagtt cgttctgcat gcgcaggctc
     1741 atgagcgcat cgagcgccga gaacggctcg tccatgtaga ggatctcggg cttgaccacc
     1801 agcgcgcgtg cgatctcggc ccgcttcaac atgccgccgg agagctcgtg cggatagttg
     1861 ttctcgaagc ctttgaggcc gaccagcgcg gcgtactcgt cggcaagctt ttcgcgattg
     1921 ggaagttcgt ctggcaaacc gaagagcaga ttctgtctga ccgtgagcca cgggaacacc
     1981 gagccctgct gcgtgatcag gactcccttg gcgttcggct gcggacgtgg cttgccgtcg
     2041 accatgatga cgcccgagtc cggcgcgaga aagccggcca tgatgttcat cagcgtcgtt
     2101 tttccgcagc ccgacggacc gacgatggcg acgaattcgc cttcacgcac ttcgaagtcg
     2161 agtccttcga tgacctgcag gttcaccttt tcgttctgaa aacccttgcg gatgttctgc
     2221 acaccgatct tcacggacga agtcttagtg tgcataacgc cacctcacga ccttcatgct
     2281 ttccagcatg cgcatgattc catcgagcag cagtccgatg agcccgatga tgatcatgcc
     2341 ggcgatcacc aggtcgtagc ggttgccggc attgcgagag tcgatgatga gataaccgag
     2401 gccggagcgc agcgcgatca tctcggcggc caccaccacc agccaggcga cgccgagccc
     2461 gatgcgcatg ccgacgatga tctgcggcag cgtggcgggg aaaaccacct ggaggaagag
     2521 cttccggcgc gacacgccga agttctccgc agcgcgcagg aaccgtttct cgatggtgtg
     2581 cacaccggcg gtcgtctgca cgatcatcgg gaacaccgaa gcgatgaaga tgaggtagat
     2641 aggcgaagcg ttgcccacgc cgaaccacag gatcgcgatc gggatccagg cgatcggcga
     2701 gatcggccgc aggatctgga acacggggtt cagcgtgacg tatgcgccac ggacccagcc
     2761 catccacagt ccgagcggta tcgcgaacaa caccgcaatg ccgaagcccg cgcccacgcg
     2821 catcagcgat gcaccgatgt gctcccacaa cgtgccgtct tcgaccagct ccatcgtgcc
     2881 ggtgaccact tgccatggtg tcggaaaaat cgcgctctgg gtggcgatga caatcgccca
     2941 ccagattccc accaccaccg cgatcacgat gatcgacggc agcgattgct tgagaccctt
     3001 catgtagctc tgttctccca ccactcacga acacgccagc gaatcttcgc ccacggcatt
     3061 accggttccg gctccaccag caccgtcatc tgtccactcg catgcgcggt gcatgcgaag
     3121 tcgtagctgg ccgctttctc gaacggcagc ctgaaactct gacccggcat catgatcgtc
     3181 ggcccgaaca cctgcggtac gtcatcgagg ttgcgcagga gcagcacgtc gttgagcccg
     3241 agtgtcaggc gaatcttcga cggcaggatc tcgaccttgt cgccggccat gcgccgcgcg
     3301 tacgtgcctt tgggtatttc aaacagctcg tcgcgcgagg gaacgctgac gggcgcgaag
     3361 cccgcccatc cgagcgccgc cagcaacgcg agcagcggca ccgcaagcaa aactcttttt
     3421 gtccggctca cgggcgaagc aggatcttga ggtcatgcac gaagtcgtcg acggtatgtc
     3481 cgtagggcat cacggctttg aggccacccg tgcgatcgat catgtagatg gatgaggagt
     3541 ggccgatggt gtaattgccg tcggcgtaga cctgcttcgt cgagctgatg ccgtaactct
     3601 tctgagccgc gtcgatatcc gctcgcgatc cgacgccacc cacgaagctg gcatcgaagc
     3661 tggcgagata tttcttcatg cgcgccgcat cgtcgcgttc cgggtccacc gtcacgtaca
     3721 ccacctggac gttggcagcg gcctcgccta acttcttgcg cgcacccgcg agcgtggcga
     3781 gcgtgatcgg acagacctcg ccgcaagagc tgaatccgaa agccagcagc acgaccttgc
     3841 cgcgataacg cgagagcgtg agcggtttac cgtcggtgcc ctgcagtttg agttcgggag
     3901 cggccatgac cggactcagc acaccggcct tcatcgcacc ggccggtgcg ggtgagttgg
     3961 cgagtgcggt ggccaggccc gccagcatca ccatcatcat ttgcagccag cgcggcatcg
     4021 gtcgcatcac agcggaatga gcgcgggctt ctgcgcgcgc acgaaacgct cgtcgatgta
     4081 ggtttcgtag gcgatcggat gcttgatggt tccggccgcg atcgacagtt gcatcagatc
     4141 ctcgaactct tcgcggatca tgcgcaggtc gccgtaggtc acgcgatcgg tcgggttctc
     4201 catcacgaac ttcaggatgt tcggatcctg gttgaagaac ccttttccgg cggcgatggc
     4261 caccgccttg tcgcggttct cctgtttctg atcgagccat tcgccggcgc tctgcacctg
     4321 gttcaccagg tcctggacca tggcggggtt ctcggcgatc agttcttcgc gcaccgtgag
     4381 gcagcagcag atgtaattgc gccactcgtc gcgtgtcatg cgcagcactc gcgcataacc
     4441 cgcgcgctgc gcggcagcgc cgaacggctc acccgtgcaa tacgcgtcga ccgcgttcgc
     4501 atacaacgcc gccggcatgt cgggcggcgg catctcgacg atctcgatgt ccttgggcgt
     4561 catgttctcg cgcgtgagca tcttgcgcag gaacaggaag tcgaccgcga agcggctcgg
     4621 gatcgcgatt cgtttaccgc gaaggtcggc gaacttctgg tacgccgaat cctggcgaac
     4681 catgatcacc gcgcccgaac gatgacccag cgagacgatc ttcaccggaa tcttcttgtc
     4741 ggcgaggtcc atcaccaaag gcgcgagcat gtacgc
//
LOCUS       DATDEU010000458         2583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_962607, whole genome shotgun sequence.
ACCESSION   DATDEU010000458 DATDEU010000000
VERSION     DATDEU010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962607"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VIV63_10985"
     CDS             <1..535
                     /locus_tag="VIV63_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6125168.1"
                     /translation="SPSRGSYLLADRDVAKLSRIELANVRNEFIGFVFQSFNLLKRTS
                     AAENVELPLIYAKVPIAERRKRAKEALDRVGLSHRAGHHPSQLSGGQQQRVAIARALV
                     TNPPLILADEPTGNLDSATTVEIMKLLTDLQQSGMTIVFVTHEPDVAAYAARKLLLKD
                     GGVVSDERQKPVLKEAS"
     gene            532..1764
                     /locus_tag="VIV63_10990"
     CDS             532..1764
                     /locus_tag="VIV63_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6125169.1"
                     /translation="MIAASETLRLAGIALTRNKMRSFLTALGIIIGVGAVIFMQSIGE
                     GAKSRVRAQIEGAGTNIVMLFSGSSQGPGGRGGQGSQPTITWEDLRAIQTELSSVAAV
                     APQMNASQPVISETNTWNTQITGTTPDFFTIRNWTVSSGAIFTASDQAGAAKNAVIGQ
                     TAAENLFGPGVDPIGQIIRIRDAPFTIIGVLERKGQNAMGSDQDDVIFIPLSAFQTRM
                     QRGLGKFVPGRIFVSAVSPEAIPRARQEIGALLRERHRLGPDDEDDFSTSSMAEMTSM
                     LTSTQDTVTTLLFSVALVSLVVGGIGVMNIMLVSVTERTREIGIRMAVGAQPADIMAQ
                     FLVEALVLAAIGGALGVIFGVGCGYLLASKFGWTMVTRTEIVVAALAVSAGVGVLFGL
                     YPARKASRLDPIEALRYE"
     gene            1839..>2583
                     /locus_tag="VIV63_10995"
     CDS             1839..>2583
                     /locus_tag="VIV63_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6125170.1"
                     /translation="MKKLSVSRRNLLLTASVAPLIISSRVWGANSRIRVGQIGCGRIA
                     QSHDMPGVIKSDMADYVAICELDAKRLADGVKIIEKSYTDRGVKPPQIRTFDNYRELV
                     ARRDIDAVVISTPDHWHAEVALAAIESGKDVYLQKPFTMTHAEGVILRDAVKRHGRIL
                     QVGSQQRSWEQFRRAAELIRSGRIGNVQRVEIGLPTDPTAPDAAPQAIPDGLDFGQWL
                     GPTPFKYYTEMRVHPQADYSRPGWLRHEAH"
BASE COUNT          459 a          853 c          826 g          445 t
ORIGIN      
        1 ttccccctcg cgtggcagct atctcctggc cgatcgggac gtcgcgaagt tgtcgcgcat
       61 cgagctcgcg aacgtccgca acgaattcat cggtttcgtg ttccagagct tcaacctgct
      121 caagcgcacg tcggcggccg agaacgtgga gcttccgctc atctacgcca aggtgccgat
      181 cgcggaacgc cgcaagcgcg ccaaggaagc gctggatcgc gtcggactgt cacatcgcgc
      241 cgggcaccat ccttcgcagt tgtcaggcgg ccagcagcag cgtgtggcga tcgcccgtgc
      301 gctggtcacc aatccgccgc tcatcctcgc cgatgaaccc acgggcaacc tcgattcggc
      361 gaccacggtc gaaatcatga agctgctgac cgacctgcag cagtcgggca tgaccatcgt
      421 gttcgtcaca cacgagccgg acgtcgccgc ctatgccgcg cgcaaactac tgctcaagga
      481 cggcggggtc gtcagtgacg aacgccagaa acccgtgctc aaggaggcct cgtgattgcc
      541 gccagtgaaa cgctgcgtct cgcgggaatc gcgctgacgc gcaacaagat gcgctctttc
      601 ctgacggcgc tcggcatcat catcggtgtc ggcgcggtca tcttcatgca gtcgatcggc
      661 gagggcgcga agtcgcgcgt gcgcgcgcag atcgaaggtg cgggcaccaa catcgtcatg
      721 ctgttctcgg gttcgagcca gggacccggc gggcgcggtg gccagggctc gcagcccacg
      781 atcacctggg aagatctgcg ggccatccag acagagctgt cgtcggtcgc cgcggtggct
      841 ccacagatga atgccagcca gccggtcatt tccgagacca acacctggaa tacgcagatc
      901 accggaacca cgccggactt cttcaccatc cgtaactgga cggtctcgag cggcgcgatc
      961 ttcaccgcgt cggaccaggc cggcgccgcg aagaatgccg tgatcgggca gaccgcggcg
     1021 gagaacctgt tcgggcccgg cgtcgatccg atcggtcaga tcatccgcat cagagacgcg
     1081 ccgttcacca tcatcggggt gctcgagcgc aagggtcaga acgccatggg ctctgaccag
     1141 gacgacgtca tcttcattcc gctgtcggcc ttccagacgc gcatgcaacg cggcctgggc
     1201 aagttcgtgc ccggacggat cttcgtgagc gcggtgtcac cggaagccat tccgcgtgcg
     1261 cgccaggaga tcggcgcctt gttgcgcgag cggcatcgcc tggggcccga cgacgaagac
     1321 gacttctcga ccagcagcat ggcggaaatg accagcatgc tgacctcgac gcaggacacg
     1381 gtgacgacgc tgctgttctc ggtggcgctg gtgtcgctgg tcgtcggcgg aatcggcgtc
     1441 atgaacatca tgctggtgtc ggtcaccgag cggacgcgcg agatcggcat ccgcatggcc
     1501 gtgggtgcgc agcccgcgga catcatggcg cagttcctgg tcgaggcgct ggtgctggcc
     1561 gccataggcg gggcgctggg cgtcatattc ggcgtgggtt gcgggtatct gctggccagc
     1621 aagttcggct ggaccatggt cacgcgcacc gagatcgtgg tcgccgcgtt ggcggtcagc
     1681 gcgggcgtcg gcgttctgtt cgggctctat ccggcgcgca aggcttcgcg gctggacccg
     1741 atcgaggcgc tgcggtacga atgatttagt cgaatacggt ttcaatttcc cgccgcttat
     1801 actcggcgtc ccttccagtt cgtacggatt ccaaccccat gaaaaagttg tccgtctcgc
     1861 gacgcaactt gctgctcaca gcatcggtcg cgcctctcat catctcttcg cgtgtctggg
     1921 gcgccaatag ccgcattcgc gtcggccaga tcggctgtgg ccgcatcgcg cagagccacg
     1981 acatgcccgg cgtcatcaaa tcggacatgg ccgactacgt cgcgatctgc gagctggacg
     2041 cgaagcgcct cgccgatggc gtcaagatca tcgagaagtc gtacaccgat cgcggcgtga
     2101 agccgccgca gatccgcacc ttcgacaact atcgcgaact ggtcgcccgc cgcgacatcg
     2161 acgcggtggt catcagcacg cccgatcact ggcatgccga agtggcgctg gccgccatcg
     2221 agtccggcaa ggacgtctat ctacagaagc ccttcaccat gacgcacgcc gaaggcgtga
     2281 tcctgcgcga tgcggtgaaa cgtcatggcc gcatcctgca ggtcggaagc cagcagcgct
     2341 cctgggaaca attccggcgc gccgccgagc tcatccgctc cggccgcatc ggcaacgtgc
     2401 agcgcgtgga aatcggactg ccgacggacc ccaccgcgcc cgacgcggcg ccgcaggcga
     2461 ttccggacgg cctcgatttc ggccaatggt taggccccac gccgttcaag tactacaccg
     2521 agatgcgcgt gcatccgcag gcggactaca gccgtccggg ctggctgcgt cacgaggcgc
     2581 att
//
LOCUS       DATDEU010000459         9685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_817313, whole genome shotgun sequence.
ACCESSION   DATDEU010000459 DATDEU010000000
VERSION     DATDEU010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9685
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817313"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..467)
                     /locus_tag="VIV63_11000"
     CDS             complement(15..467)
                     /locus_tag="VIV63_11000"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY6125171.1"
                     /translation="MLDSGDRAPEFTLPDQDGHDISLTALLKDGPAILFFYPADFTPT
                     CTREVCSIRDIHKEISRAGITVVGISPQDPASHKRFREKHNLPYSLLADEKKDVIKMF
                     GVNGPLGFWVQRVTFLVDQNRVVRGRVKATFSVDEHEAFIRQAIELRG"
     gene            567..642
                     /locus_tag="VIV63_11005"
     tRNA            567..642
                     /locus_tag="VIV63_11005"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Ala,seq:cgc)
     gene            complement(651..1433)
                     /locus_tag="VIV63_11010"
     CDS             complement(651..1433)
                     /locus_tag="VIV63_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY6125172.1"
                     /translation="MPVPRNYRIGEFSRLSGTSIKTLRFYDEIGLLRPDVVDARTRYR
                     YYNAGQLRDLAAICALKDLGASLADIRRVMSLKDARSERRKLLAKLRTIAQRTLAATS
                     RSLDWIELELDDSGQSLAGTPVVLKQRGEMRIASIRARLSHYADIAPVEHDLMQALNP
                     GAACEQRGVLWHRCEASGAIDGEPFVEVRGAAPRGCGIEIRSLTGGRVASAYCESDDD
                     AAVRTYDAIDRWIHRHELRLDGPKREIYVGQLLEIQFPVRPA"
     gene            1488..2519
                     /locus_tag="VIV63_11015"
     CDS             1488..2519
                     /locus_tag="VIV63_11015"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125173.1"
                     /translation="MNRRGFLQVLSAASISAAMSGCAARRADHGDWIMTVRGKRPAHD
                     LGRTLTHEHALANFQPYEEWLRAPRTYDRAAVVKRVLPHLQRIAALGCRSCVDATAVG
                     LGRDPQLLRQLSEESGLNILTATGNYAAFDYRFLPQYVRDESVEVLASRWIREFTDGI
                     DGTEVRPGFIKLGFNGGSLSDVERKLIRAGAVAHRATGLTIGAHTGMAVAAYEQLAVL
                     EAAGVHASAWIWIHAQNEPDPAKYDDAARRGAWISLDGVSADSIAVHVDRVVRLRDSG
                     LLHRVLVSQDAGWYWVGEPDGGKFRPYDTVFTDFIPALKARAFSEAEIDQIFVKNPAS
                     AFAVRVRTL"
     gene            2535..2610
                     /locus_tag="VIV63_11020"
     tRNA            2535..2610
                     /locus_tag="VIV63_11020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2568..2570,aa:Arg,seq:cct)
     gene            complement(2625..2924)
                     /locus_tag="VIV63_11025"
     CDS             complement(2625..2924)
                     /locus_tag="VIV63_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY6125174.1"
                     /translation="MCQTASGSVGLKAALRRLLLMEVRNMSTLKAMSLAESREIFDLC
                     DLNGDGLIDHDEFHVLLEVLDGDVSRAECLLDFEVADTAGDGYIEFREFVAWWTN"
     gene            complement(2931..3314)
                     /locus_tag="VIV63_11030"
                     /pseudo
     CDS             complement(2931..3314)
                     /locus_tag="VIV63_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
     gene            3377..4297
                     /gene="kdgD"
                     /locus_tag="VIV63_11035"
     CDS             3377..4297
                     /gene="kdgD"
                     /locus_tag="VIV63_11035"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF002958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HEY6125175.1"
                     /translation="MGMKPSDMATRIGRGLLSFPVTHFDAEDRFHETAYRQHCSWLLE
                     HEVAGLFAAGGTGEFFSLKPSEVGAVVAAAVAETKGRVPVIAGCGYGTAMAVELARDV
                     EKRGADGILLLPPYLVNSEQQGLAAHIDKVCKSVSIGVIVYNRDNAILTDETLAKLCE
                     GNANLVGFKDGVGDIETLTRIRSRMGDRLTYIGGMPTAETFALPYRDIGITTYSSAIF
                     NFMPTWALRFYGAVQAGDRATVQHHLKDFVMPYIAIRNRAKGFAVSIVKAGCRIVGRP
                     AGHVRSPLTELGPKDLDDLRALIKRAESLA"
     gene            complement(4299..6161)
                     /locus_tag="VIV63_11040"
     CDS             complement(4299..6161)
                     /locus_tag="VIV63_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125176.1"
                     /translation="MNQEQQNGLSRRAFVAGTGALALASRSLGASTRPELHATSPMPA
                     PEWALLQRELLRTHTEACEIFHAKYFDERHHLRCFPRWGTNDGPDDAIEHVNDWPLVH
                     ALGGSDRILELYRAVYEGHIEQYSALRTRDVPMGREGMYVREFPPQMDWQHISEGLSV
                     FNLMGLSTPGDTRLIERTRRFAGFYDGSDPAAPNYDRKHRVIRSMFNGSIGPLLRPTT
                     ALDWAGDPFDASRFKLEHGERNFDEFLAHFQEYTDTVCDSPLNLQSTTLALNAYLLTG
                     EKRWRDWLLSYVDAWVERAAANDDILPSKVGLNGRIGGKSGRWYEGVYGWAFSPVVPQ
                     TGKREDRNRVPRSIVAFMNAALLTGDDRYMAVWRRQNAAINFKARDIDGQSQTPRMCG
                     PKGWYSYVPGPYRANAFEIWYVSMNPADRALAGSHPWVDYLEGRNPAYPVTALRASLE
                     RVRARVALVNADTSTPETRLADNALEYNPASVASLLQLTQGALHIARPPWSPTSPHQG
                     GAPLHARLRYFDPVSRRAGLPEDLAALVDTLDAARTGVTLVNPNPGRERVVTVQGGAY
                     GEHVISTVHDGQKSHAVNGRSFDVKLGPGCGARLELTMQRYGGKPSLAFPWDRA"
     gene            6234..6653
                     /locus_tag="VIV63_11045"
     CDS             6234..6653
                     /locus_tag="VIV63_11045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY6125177.1"
                     /translation="MNQAMHVLRVRASRRGLIEFTGDVRRWLEQQQIAAGLLTLFCRH
                     TSASLLIQENAAPAVRSDLEKFFDGLAPEGAGLYEHDDEGPDDMPAHLKTALTQVQIS
                     VPVMDGKLMLGTWQGIYLFEHRDHPSQREIVLHLTGN"
     gene            6668..7561
                     /locus_tag="VIV63_11050"
     CDS             6668..7561
                     /locus_tag="VIV63_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6125178.1"
                     /translation="MRTIAAACLALLAATAEAAVPAPTRIVVNGVELHYISAGAGESV
                     ILLHGGQGDYRSWEPQMAALSRHYHVISYSRRYHFPNVNPQTATNHSVLVEAADLEAL
                     VKALRLKRPNLVGTSMGAATALTLAVQHPKMFRSLVLAEPPLLAWAKRFPDGGKLYED
                     FMVHIQNPARDAFAAGDNEAAMRFFVDGFATPGRFDKLTPDARLGVMQNAGFFRMMTR
                     SENPYPEIPRQALWKLRIPVLVITGEKTTEIHRRIDEELARMTPRARSATIPNAGHGS
                     PRENPGAFTEVVENFLETSGK"
     gene            7685..9259
                     /locus_tag="VIV63_11055"
     CDS             7685..9259
                     /locus_tag="VIV63_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125179.1"
                     /translation="MTEDRTLPPPAYIGPLFRADALALRYGSAIVVVLLIAGIRALLA
                     PLLGTQAPLLPFVLGVFAAAYLGGRGPALLASLLTPILATLWFTNWPQDAPPLQWISH
                     VVFFLLIAALSAQLMHELQKRSRTEREALAMAASHAQALQEADRRKDEFLAMLAHELR
                     NPLAPIRNVAYVLTKGMADNATVRRSAQMLERQANHLTHLVDDLLDVARITRGRVVLK
                     RETLSLDSIAESALETVQPLLDARSQTVSLRRQAEQVFVDADGVRLCQVVANLLTNAI
                     KYSPERSRIDIVIDASADDASISVHDPGVGIDAQLLPLLFDPFLQGDRTLDRAQGGLG
                     VGLTIVKLLVEMHGGAVSAYSAGLGKGSEFRIRLPKVPAPVAVSAPTPQDEHRTVRPR
                     RVLVIEDNADAAESMRAVLRTDRHEVEVVYDGAAGLAKLEDFRADIVLLDIGLPRMDG
                     YMVAHAIRERFALVGRRPKLLALTGYGRDDDRNSALKAGFDGHLAKPVDPRRLLEIVA
                     DEPQARNTSAASKQRG"
     gene            complement(9264..>9685)
                     /locus_tag="VIV63_11060"
     CDS             complement(9264..>9685)
                     /locus_tag="VIV63_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6125180.1"
                     /translation="RRGRVLVADDNRDAAESLALVLRFSGYEVSIAFGGAEALEIGQR
                     ERPRAAIIDIGMPGMSGHEVARRMRLEAWGRNSVLIALTGWGQEQDKQAAKAAGFDAH
                     LTKPVDPDDLERVLDELLSDAPEDSGKDGVADTLRNA"
BASE COUNT         1668 a         3182 c         3094 g         1741 t
ORIGIN      
        1 tctaccccct ttttctaccc gcgcagctcg atggcctgcc tgatgaacgc ctcgtgttca
       61 tcgaccgaga aggtcgcctt cacccggcct cgcaccacgc ggttctggtc caccagaaac
      121 gtgacccgct gcacccagaa ccccagcgga ccgttcaccc cgaacatctt gatgacatcc
      181 ttcttctcgt cggccagcag cgagtagggc aagttgtgtt tctcgcgaaa ccgcttgtgg
      241 ctcgccgggt cctgcgggct gatcccgacc accgtgatgc cggcgcgact gatttccttg
      301 tggatatccc ggatcgagca gacttcgcgc gtgcaggtgg gggtgaagtc cgccgggtag
      361 aaaaagagga tggccggccc gtccttgagc agggcggtca gtgaaatatc gtgcccgtcc
      421 tggtccggaa gcgtgaattc gggggccctg tcgccggagt caagcatgcc gtggatatta
      481 agcccttgcc cgtgacagtc gggattactt tccctacaat cccgcgcctt cccggacccc
      541 caaatccggg accggtttct acgtgtgggg cggtagctca gatgggagag cgtcgcgttc
      601 gcaatgcgaa ggtcgagggt tcgatcccct tccgctccac catttcctct tcaggccggc
      661 cttaccggga actggatctc cagtaactgc cccacgtaga tctcgcgctt ggggccatcc
      721 agccgaagtt catgccggtg tatccatcga tcgatggcat cgtaagtgcg cacggcggca
      781 tcgtcgtcgg actcgcagta cgcgctcgcc acccgtccgc ccgtgaggct cctgatctcg
      841 atgccgcaac cgcgcggcgc agcgccccgg acttccacga atggttcacc atcgatggcg
      901 cccgacgctt cgcatcgatg ccacaaaacg ccccgctgct cgcaggccgc gccgggattc
      961 agcgcctgca tgagatcgtg ctcgacgggc gcgatgtccg cgtaatggct caaccgcgcg
     1021 cgaatcgacg cgatgcgcat ttcgccgcgc tgcttgagaa cgacgggcgt gcccgcgagc
     1081 gattggcctg aatcgtcgag ctcgagctcg atccagtcga gcgaacgact cgtggcggcc
     1141 agcgtgcgct gggcaatggt gcgcagcttc gccagcaatt tgcgccgctc gctgcgcgca
     1201 tccttgagcg acatgacgcg gcggatgtct gcgagcgacg cgccgagatc cttgagcgcg
     1261 cagatcgcgg cgagatcgcg aagctggccg gcgttgtaat aacggtagcg tgtgcgtgcg
     1321 tcgaccacgt cgggccgcag caggccgatc tcgtcgtaga agcgcagcgt cttgatggac
     1381 gtgccgctca gcctcgagaa ttctccgatc cggtaattcc tgggaactgg catcttgaag
     1441 tctccaccgc gctggagtgt tctgattgaa ggcaggagga tcgtcccatg aatcgcagag
     1501 gttttcttca ggtcctgtcc gcggcctcga tttccgccgc catgagcggc tgcgcggccc
     1561 ggcgcgccga ccacggcgac tggatcatga cggtgcgcgg aaagcggccg gcgcacgatc
     1621 tgggccgcac actgacccac gagcatgcgc tcgccaattt tcagccctac gaggaatggc
     1681 tgcgcgctcc gcgaacgtac gatcgcgccg ccgtcgtgaa acgggtgctg ccgcatctgc
     1741 agcgcatcgc cgcgctcggc tgccgcagct gcgtcgacgc aacggccgtg gggctcggcc
     1801 gtgacccgca gcttctgcgc cagctgtcgg aagagagtgg cctgaacatt ctcactgcca
     1861 ccggaaacta cgcggccttc gactatcgtt ttcttccgca gtacgtgcgc gacgagtcag
     1921 tcgaagtgct cgcctcgaga tggatacgcg aattcaccga tggcatcgac ggcacggaag
     1981 tccgcccggg attcataaag ctcggcttca atggtggctc gctcagtgac gtggagcgca
     2041 agctcattcg tgccggcgcg gtcgcgcatc gggccaccgg gctcaccatc ggcgcacaca
     2101 ccgggatggc tgtcgcggcc tacgagcaac ttgcggtgct cgaggccgcg ggtgtccatg
     2161 cctccgcctg gatctggata cacgcgcaga acgaaccgga tcccgcgaag tacgacgatg
     2221 cggccaggcg cggagcgtgg atttcgctcg atggcgtcag tgccgacagc atcgccgtgc
     2281 atgtcgatcg agtggtgcgg ctgcgcgact cgggactcct gcaccgcgtg ctcgtgtcgc
     2341 aggatgcggg gtggtattgg gtcggggagc ccgacggcgg aaaattccgt ccctatgaca
     2401 ccgtattcac ggacttcatc ccggctttga aggcccgggc cttctcggaa gccgagatcg
     2461 accagatttt cgtgaagaac ccggcatccg cgttcgcggt gcgcgtgcgc acgctgtaaa
     2521 attcgcggcc tcgcgccccc gtagctcagt ggatagagcg accgcctcct aagcggtagg
     2581 tcgtcggttc aactccggcc gggggcgcca gttcgattcc tcactcaatt cgtccaccac
     2641 gcgacgaatt cccgaaactc gatgtagcca tcgcccgcgg tgtcggccac ctcgaagtcg
     2701 agcaggcatt cggcacgaga cacgtcaccg tcgaggacct ccagcaggac gtggaattca
     2761 tcgtgatcga taaggccgtc gccattcaga tcgcacaaat cgaagatttc gcgcgactcc
     2821 gcaagactca tggctttcag ggtgctcatg tttcgaacct ccatgagaag caatctacgc
     2881 agggctgcct taagcccaac tgaacccgat gccgtttgac agattgcggc ctaacgcgaa
     2941 atcaggcgcc aggtgatcca cgcggcaacg gccgcgagca ggatccagat cgtgacgatc
     3001 gcggccgcac ctgccttgct gacgggccgc tgtgccgccg cgtcggcagt cgcacgatgt
     3061 tcggccatga tcgctggcgg aatcattcgc accgcaagcc agatgcccag aggcacgatg
     3121 atcaggtcat cgagataccc caacaccgga atgaagtcgg ggatcagatc gatgggcgag
     3181 agcgcgtaac cggccaccag cactgcaacg gccttcgccg gccacggcac acgcgagtcg
     3241 cgggccgcga ggtagagggc atgcacgtcg cgtttgatcg cacgtgccca gcgtttgagt
     3301 cgatcgagca cgcggtgatt gtgtcatgcg ggcgcgcgct ggtgcgcctc gccggcttgc
     3361 actagagtcc gccgccatgg gcatgaaacc ttctgacatg gccacccgca tcggacgcgg
     3421 cctgctgtct tttcccgtca cgcatttcga cgcggaagac cgctttcacg agaccgccta
     3481 ccggcaacac tgctcgtggc tgctcgaaca cgaagtggcg ggacttttcg ccgccggcgg
     3541 cacgggtgaa ttcttttcgc tgaagccttc cgaagtcggc gcggtcgtcg cagcggcggt
     3601 cgcggagacg aagggccgcg tgccggtgat cgcgggctgc ggatacggca ccgcgatggc
     3661 ggtcgaactc gcgcgcgatg tcgagaagcg cggcgccgat ggcatcttgc tgctgccgcc
     3721 gtacctcgtg aactccgagc aacagggtct cgcggcgcac atcgacaaag tctgcaagtc
     3781 cgtgtcgatc ggcgtcatcg tctacaaccg tgacaacgcc attctcaccg acgagacact
     3841 ggccaaactc tgcgagggca atgccaacct cgtgggcttc aaggacggcg tcggtgacat
     3901 cgagacgctc acgcgcattc gcagccgcat gggcgaccgg ctcacctaca tcggtggcat
     3961 gccaacggcg gaaaccttcg cgctgccgta tcgcgacatc ggcatcacca cgtattcctc
     4021 ggccatcttc aacttcatgc cgacgtgggc gttgaggttc tacggcgccg tgcaggcggg
     4081 cgaccgcgcc accgtgcagc accacctcaa agatttcgtg atgccgtaca tcgcgatccg
     4141 caatcgcgcc aaaggattcg cggtgtcgat cgtgaaagcg ggatgccgaa tcgtcggccg
     4201 gcccgcgggc catgtgcgct cgccgttgac ggagctgggc ccgaaggacc tcgacgatct
     4261 gcgcgccctc atcaagcgcg ccgagtcgct ggcctagatc aggcgcggtc ccagggaaaa
     4321 gccagcgatg gctttccgcc gtagcgctgc atcgtgagtt cgaggcgcgc gccgcaaccc
     4381 ggccctaact tcacgtcgaa gctgcgtccg ttcacggcgt gggacttctg tccgtcgtgc
     4441 accgtcgaaa tcacatgctc gccatacgcg ccaccctgaa ccgtgacgac gcgctcgcgg
     4501 cctggattcg ggttgaccag cgtcacgccg gtgcgcgccg cgtcgagcgt gtcgacgagc
     4561 gcggccaggt cttccggcaa gccggcccgg cgactcaccg gatcgaaata acgtagtcgt
     4621 gcgtgtaacg gcgcgccacc ctggtgaggc gacgtgggtg accacggcgg tcgcgcaatg
     4681 tgcagggctc cctgcgtgag ctgcagcagc gacgccacgc tggccgggtt gtattcgagc
     4741 gcgttgtcag cgagtcgggt ctcgggtgtc gaggtgtctg cattcaccag cgcgactcgc
     4801 gcgcgaaccc gctccagcga tgcgcgcagc gctgtgacgg gataggcagg gttgcggcct
     4861 tcgaggtaat cgacccacgg atggcttccc gcgagcgcac gatccgcggg attcatcgac
     4921 acgtaccaga tctcgaacgc gttcgcgcga tacggcccgg gcacgtagct gtaccagcct
     4981 ttcggcccgc acatacgcgg cgtctgcgac tggccgtcga tatcccgggc cttgaagttg
     5041 atcgccgcgt tctgccggcg ccacacggcc atgtagcgat cgtcgcccgt cagcagcgcg
     5101 gcgttcatga acgccacgat ggagcgcggt acacgattgc ggtcttcgcg tttgccggtc
     5161 tgcggcacga cgggactgaa cgcccagccg tacacgcctt cgtaccagcg gccactcttg
     5221 ccaccgattc ggccgttgag cccgactttg ctcggcagga tgtcgtcgtt ggcggcagcg
     5281 cgctccaccc acgcgtcgac gtacgacagc agccagtctc gccagcgctt ctctcccgtc
     5341 aatagatagg cattgagcgc gagggtcgtg gactgcaggt tcagcggact gtcgcaaacc
     5401 gtatcggtgt attcctggaa gtgcgcgagg aactcgtcga agttgcgctc gccgtgttcc
     5461 agcttgaaac gggaggcatc gaaaggatcg ccggcccagt cgagcgcggt ggtcggacgg
     5521 agcagggggc cgatgctgcc gttgaacatg ctgcggatga cgcgatgttt gcggtcatag
     5581 ttgggcgcgg ccgggtcgct gccgtcgtaa aaccccgcga agcgacgcgt gcgttcgatg
     5641 agtctcgtgt cacccggcgt cgaaaggccc atcagattga agacggacaa gccttcgctg
     5701 atgtgctgcc agtccatctg cggcggaaac tcacgtacgt acatgccttc gcggcccatc
     5761 ggaacgtccc gggtgcgcaa cgcggagtac tgctcgatgt gcccttcgta gacggcgcga
     5821 tacaactcga ggatgcgatc cgaaccgccg agcgcgtgca cgagcggcca gtcgttcaca
     5881 tgttcgatgg cgtcgtcggg gccgtcgttg gtgccccagc gcgggaagca acgcaggtga
     5941 tggcgctcgt cgaaatactt cgcgtggaag atctcgcagg cttcggtgtg cgtgcgcagt
     6001 agttcgcgct ggagcagcgc ccactcgggg gcaggcatgg gagacgtggc gtgcagctcc
     6061 ggacgggtgc ttgcgcccaa cgatctcgat gcgagcgcca gggctccggt gccggccacg
     6121 aatgcacggc ggctgagccc gttctgttgt tcctggttca agcttccccc tgcgcgctcc
     6181 cggggatcat aatctccgcg catgccccaa gtccctatgc cgcgacatac ccgatgaatc
     6241 aggccatgca tgtcctgcgc gtgcgggcga gccgccgggg cttgatcgaa ttcaccggcg
     6301 atgtgcgtcg ctggctggag caacagcaga tcgccgccgg actgctcacg ctgttctgcc
     6361 gtcacacgtc tgcgtcgctg ctcatccagg aaaatgccgc ccccgccgtg cgcagcgatc
     6421 tcgaaaaatt cttcgacggc ctcgcgcccg aaggcgccgg gctctacgaa cacgacgacg
     6481 agggccccga cgacatgccg gcgcatctca agaccgcgtt gacgcaggtg cagatctcgg
     6541 tgccggtcat ggacggcaag ctcatgctcg gcacctggca gggcatctat cttttcgaac
     6601 accgcgatca tccctcgcaa cgcgagatcg tgttacacct gacggggaac tgaagggagt
     6661 gcgcgatttg agaactattg cggccgcttg cctggccttg ttagccgcga cggcagaggc
     6721 cgcggtgccg gcgccgacga ggatcgtcgt caacggcgtc gagttgcatt acatcagcgc
     6781 cggtgccggc gaatccgtga tcctgctgca tggcggccag ggcgactatc ggtcgtggga
     6841 accgcagatg gccgcgctct cgcggcacta ccacgtcatc tcgtacagcc gccgctacca
     6901 ctttcccaac gtgaacccgc agaccgcgac caaccattcg gtgctcgtcg aggcggcgga
     6961 cctcgaggcg ctggtgaagg ccctgcgtct caagcgcccg aacctcgttg gcacctcgat
     7021 gggcgcggcg acggcactca cgctcgcggt ccagcacccg aagatgttcc gcagcctggt
     7081 gctggccgag cccccattgc ttgcctgggc caagcgcttt cccgatggcg gcaagctcta
     7141 cgaagatttc atggtgcaca ttcagaatcc ggcgcgtgac gcgttcgccg ccggcgacaa
     7201 cgaggcggcc atgcgcttct tcgtcgacgg cttcgcgacg ccgggacgct tcgacaagct
     7261 cacgcccgac gcgcgcctcg gcgtcatgca gaacgccggc ttcttccgga tgatgacgcg
     7321 cagtgagaat ccatatcctg agattccgcg gcaggcttta tggaaattgc gaattccggt
     7381 gctcgtgatc accggtgaga agaccaccga gatacaccgg cgcatcgacg aagaacttgc
     7441 tcgcatgact ccccgcgcca ggtccgcgac catacccaat gcgggccacg gatctccgcg
     7501 cgagaatccg ggggcgttta cggaagtggt cgagaatttt ctggagacca gcgggaaatg
     7561 aagtttatgt ccgctttgcg ccggaacatt cctacaattc gctgagcgcg actcctaatt
     7621 ccggcggcgt taaagttcga ctcgacggtc acgcgaagtc gcgttagcgc cgagcaagga
     7681 atcgatgacc gaggatcgta ctcttccgcc tccggcgtat atcgggcccc tgtttcgagc
     7741 cgacgcactg gcattgcgtt atggctccgc gatcgtcgtc gtgctgctca tcgccggaat
     7801 ccgcgcgctg ctcgcgccct tgttaggcac gcaggcgccg ttgctgccgt tcgttctcgg
     7861 agtgttcgcc gctgcctacc tgggtgggcg cgggcccgcg ctgctggcca gccttctcac
     7921 gcccatcctc gccaccttgt ggttcacgaa ctggccgcag gatgcgccgc cattgcagtg
     7981 gatctcccat gtggtgttct tcctgctgat cgcagcgctg tcggcccagc tcatgcacga
     8041 actccagaag cgctcgcgca ccgaacgcga ggcgctcgcc atggccgcga gccacgcgca
     8101 ggcgctccag gaagccgacc ggcgaaagga cgaattcctg gcgatgctgg cgcacgaact
     8161 gcgcaacccc ctcgcgccca tccgcaacgt cgcgtacgtg ctcacgaagg gcatggccga
     8221 taacgcgacg gtgcgccgat cggcccagat gctcgagcgc caggccaatc acctcactca
     8281 tctcgtcgac gatctgctcg atgtcgcgcg catcacgcgc ggccgcgtgg tgctgaaacg
     8341 cgagacgttg agtctcgact cgatcgccga gtccgcgctc gagaccgtgc agccgctgct
     8401 cgatgccaga agtcagacgg tgagcctccg gcgccaggcg gagcaggtct tcgtggacgc
     8461 agacggcgtg cgcctctgcc aggtggtcgc gaatctcctg accaacgcga tcaagtattc
     8521 gcccgagcgg tcccgcatcg atatcgtcat cgacgcgtcg gcggacgacg catccatcag
     8581 cgtgcacgat ccgggcgtcg gcatcgacgc gcaattgctg cccttgctgt tcgacccgtt
     8641 cctgcagggc gatcgcacgc tcgatcgtgc gcagggtggt ctcggcgtgg ggctgacgat
     8701 cgtgaagctg ctggtcgaga tgcacggcgg cgccgtgtcg gcgtacagcg cgggcctcgg
     8761 taaaggcagc gagttcagga ttcgcctgcc gaaggtgccg gcgccggtgg ccgtgtccgc
     8821 gccaacacct caggacgaac atcgcacggt gcgcccgcgc cgcgtgctgg tgatcgagga
     8881 caacgcggac gccgccgagt cgatgcgcgc ggtgttgcgc accgaccgtc acgaggtcga
     8941 agtcgtgtac gacggcgcgg cgggcctcgc caagctcgaa gattttcgcg cggacatcgt
     9001 gttgctcgac atcggactgc cgcgcatgga cggctacatg gttgcgcacg ccattcgcga
     9061 gcgatttgcg ctggtcggcc ggcgcccgaa gttgctggcg ctgaccggtt acggacgcga
     9121 cgatgaccga aactcggctc tcaaggccgg attcgacggg cacctggcca agcctgtgga
     9181 cccgcgccgt ctgctcgaga tcgtcgccga cgaacctcag gcgcgtaaca cgagcgccgc
     9241 gtcgaaacag cgtggctgac cgatcaggcg ttacgcaggg tatccgcgac gccgtctttg
     9301 ccggaatctt ccggcgcgtc gctcagtagt tcgtcgagca cgcgttcgag atcgtcggga
     9361 tccaccggtt tggtcaggtg tgcatcgaac cccgcggcct tggcggcttg cttgtcctgc
     9421 tcctgccccc agccggtgag cgcaatcaac acggagttgc ggccccacgc ttccagtcgc
     9481 atgcgccgcg cgacttcgtg tccgctcatg cccggcatgc cgatgtcgat gatcgccgcg
     9541 cgcggccgct cgcgctgacc gatctcgagc gcttcggcgc cgccgaaagc gatcgacact
     9601 tcgtaaccgg agaagcgcag caccagcgcc agtgattcgg ccgcgtcgcg attgtcgtcg
     9661 gcgacgagca ctcgcccacg ccgca
//
LOCUS       DATDEU010000460         3188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1983393, whole genome shotgun sequence.
ACCESSION   DATDEU010000460 DATDEU010000000
VERSION     DATDEU010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983393"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VIV63_11065"
     CDS             complement(<1..180)
                     /locus_tag="VIV63_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125181.1"
                     /translation="MAEQKGIDAAHRRVIFASSLGTVFEWYDFYLYGSLVTVITKKFF
                     SGMNDTTAFLFALLAF"
     gene            344..>3188
                     /gene="uvrA"
                     /locus_tag="VIV63_11070"
     CDS             344..>3188
                     /gene="uvrA"
                     /locus_tag="VIV63_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY6125182.1"
                     /translation="MRQPRSPRKPGKHVNTRACIAVHGARQNNLKNLSLEIPLHELVV
                     VTGVSGSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPAVDRIDGIPPAIAID
                     QTNPVRTSRSTVGTMTELNDHLKLLFARAGTLHCRNCGKPVRRDSAESIFADATARAA
                     AASDPRVYICFPVTVPKNFKEAEVRQLLESQGYSRIHERHGDTLEVVQDRLRLGNADR
                     SRVMESLETALRVGRGRVNLQVVDESPSSVEVGPKSIERWKYSNELHCADCDLSYRDP
                     VPSLFSFNSPVGACESCRGFGRIIGIDYGLVVPDETKTLAGGAVKPWQTKSYKECQDD
                     LVKFANKRDIPLDTPWYALTDAQREWVIEGEGKWEKKVWYGAKRFFEWLETKAYKMHV
                     RVLLSRYRAYTPCTTCNGSRLKPDALLWRIDGRNVHDLVLMSLDKSRVFFDQLKLPAP
                     LDEAADLLLGEVRSRFAYLCEVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTL
                     FVLDEPSIGLHPRDMQRVTQVMHRLRDAGNSLVVVEHDPQIMLAADRLLDLGPGAGER
                     GGEIVAFDTPDAVRANPKSLTGQYLSGAKVVAPRATPAPAHEPKVREVKAHMVPAQRS
                     IRLRGVTQHNLKNIDVEFPLRRMVCITGVSGSGKSTLIEDVLYKALRKAKGQPSEAPG
                     AFERLEGAEQVDDVVMIDQSSIGRTTRSNPASYVGAFDVVREWFANEPVAKARKYTPG
                     TFSFNSGTGRCPTCSGNGFEHVEMQFLSDVYLRCPDCDGKRYRAETLEIRREGADGTR
                     RNIAQVLDMTVTEALAFFRGNSNVAIRLQPLVDVGLEYVKLGQPVPTLSGGEAQRLKL
                     AGHLAEAAADDTPSIRGKLFLFDEPTTGLHFDDVAKLLGAFKRLLDAGHSLVVIEHNL
                     DVIAASDWIVDLGPEGGEAGGAVVCTGMPADVRK"
BASE COUNT          582 a         1053 c         1014 g          539 t
ORIGIN      
        1 gaacgccaac agcgcgaaca ggaacgccgt ggtgtcgttc atgcccgaga agaacttctt
       61 ggtgatgacg gtgaccagcg agccgtagag gtagaagtcg taccactcga ataccgtgcc
      121 cagcgaagac gcgaagatga cgcgccggtg tgcggcgtcg attccttttt gttctgccac
      181 gaaaccccct gttgtggcgc ttacgatact tgaagccttt gcaaatcgcc gcatttcccc
      241 ggacccggcg ttgcaccccg cctccgccac caggatttcc agacaacccg cttgacatgg
      301 ccggtacaat gaccgatccg cgtcatcgca gaaccttcga tacatgagac agccccgttc
      361 cccacgcaag cccggcaagc acgtgaatac gcgcgcctgc atcgccgtgc atggcgcgcg
      421 ccagaacaac ctcaagaatc tgagtctgga gattccgctg cacgaactcg tggtggtcac
      481 gggtgtgtcg ggttcgggca agtcgtcgct ggtgttcgac acgctgtacg ccgaaggcca
      541 gcgccgctac gtcgaaacct tctcgccgta cgcgcggcag ttcctcgacc gcatggacaa
      601 gccggcggtc gatcgcatcg acggcattcc acccgccatc gccatcgacc agacaaatcc
      661 ggtgcgcacc tcgcgctcta ccgtcggcac gatgaccgag ctcaacgatc acctgaagct
      721 gctgttcgcg cgcgccggca cgctccactg ccgcaactgc ggcaagcccg ttcgccgcga
      781 cagcgcggaa agcatttttg ccgacgccac cgcgcgcgcg gcggccgctt cggatccgcg
      841 cgtgtacatc tgcttcccgg tcacggtccc gaagaacttc aaggaagccg aagtccggca
      901 actgctcgaa tcgcagggtt attcgcgtat tcacgaacgt catggcgaca cgctcgaagt
      961 cgtgcaggac cgcctgcgcc tcggaaacgc cgaccgctcg cgcgtcatgg agtcgctgga
     1021 gacggcgctg cgcgtcggcc gcggccgcgt caacctgcaa gtggtcgacg agagcccctc
     1081 ttccgtggaa gtagggccga agtccatcga gcgctggaag tattcgaacg aactgcattg
     1141 cgcggactgc gatctttcgt atcgcgaccc ggtgccttcg ctgttctcgt tcaactcgcc
     1201 ggtcggcgcc tgcgaatcct gccgcggttt cggccgcatc atcggcatcg actacggcct
     1261 ggtggtgccc gacgaaacga agacgctcgc cggcggcgcg gtcaaaccct ggcagacgaa
     1321 gtcgtacaag gagtgccagg acgacctcgt gaagttcgcg aacaagcgcg acatcccgct
     1381 cgacacgccg tggtacgcgc tcaccgacgc gcagcgcgaa tgggtcatcg aaggcgaagg
     1441 caagtgggag aagaaagtct ggtacggcgc caagcgcttc ttcgagtggc tggaaaccaa
     1501 ggcctacaag atgcacgtgc gcgtgttgct gtcgcgttat cgcgcgtaca cgccctgcac
     1561 cacctgcaat ggctcgcgcc tcaaacccga cgcgctgttg tggcgcatcg acggccgcaa
     1621 cgtgcacgac ctcgtgttga tgtcgctgga caagtcgcgc gtgttcttcg accagttgaa
     1681 actgccggcg ccgctcgatg aggccgccga cctgttgtta ggcgaagtgc gctcgcgctt
     1741 cgcgtacctg tgcgaggtgg gtctcggcta tctcacgctc gatcgtcagt cgcgcacgtt
     1801 gtcgggcggc gaagtccagc gcatcaatct cacgaccgcg ctcggcacct cgctggtgaa
     1861 cacgctgttc gtgctggacg agccgtccat cggactgcat ccgcgcgaca tgcagcgcgt
     1921 gacgcaggtc atgcaccggc tgcgcgacgc cggcaattcg ctggtggtgg tggaacacga
     1981 cccgcagatc atgctcgcgg ccgaccggct gctcgatctc gggcctggcg ccggcgaacg
     2041 gggcggcgaa atcgtggcgt tcgatacgcc cgatgccgtg cgcgcgaatc ccaaatcgct
     2101 gaccgggcaa tacctgtcgg gcgccaaggt cgtcgcgccg cgcgcgacgc cggcacctgc
     2161 gcacgagccg aaagtgcgcg aggtcaaggc gcacatggtg ccggcgcagc gttccatcag
     2221 gctgcgcggc gtcacgcaac acaacctcaa gaacatcgac gtggaattcc cgctgcggcg
     2281 catggtgtgt atcaccggcg tgtcgggctc gggcaaatcg acgctcatcg aggacgtgct
     2341 gtacaaggcg ctccgcaagg ccaagggcca gcccagcgag gcgcccggcg cgttcgaacg
     2401 gctcgaaggt gccgagcagg tcgacgacgt cgtcatgatc gaccagagct cgatcggccg
     2461 aactacccgc tcgaatcccg cgagttatgt cggcgcgttc gacgtggtgc gcgagtggtt
     2521 tgccaacgaa ccggtggcca aggcgcgcaa gtacacgccg ggtactttca gcttcaactc
     2581 cggcacgggc cggtgcccca cgtgcagcgg caacggcttc gagcacgtgg agatgcagtt
     2641 cctctcggac gtctacctgc gttgcccgga ctgcgacggc aaacgttatc gcgccgagac
     2701 gctcgagatc cgtcgcgaag gcgcggacgg cacgcgccgg aacatcgcgc aggtgctcga
     2761 catgacggtc accgaggcgc tggcgttttt ccgcggcaat tcgaacgtgg ccattcgcct
     2821 gcagccgctc gtggacgtgg gccttgaata cgtgaagctg ggccagcccg tgcccacgtt
     2881 gtcgggtggc gaggcgcagc gactgaaact cgcgggccac ctggccgagg ccgccgccga
     2941 cgacacgccg tcgatccgcg gcaagctgtt cctgttcgac gaaccgacca ccggcctgca
     3001 tttcgacgac gtcgccaagc tcctcggcgc gttcaagcgc ctgctcgacg ccgggcattc
     3061 actggtcgtg atcgaacaca acctcgacgt aatcgccgcg tccgactgga tcgtcgatct
     3121 gggtcccgaa ggcggcgagg cgggaggggc tgtggtttgt accggcatgc cggcggatgt
     3181 caggaagc
//
LOCUS       DATDEU010000461         4405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1400592, whole genome shotgun sequence.
ACCESSION   DATDEU010000461 DATDEU010000000
VERSION     DATDEU010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400592"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..3758
                     /locus_tag="VIV63_11075"
     CDS             216..3758
                     /locus_tag="VIV63_11075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HEY6125183.1"
                     /translation="MTTTVSGWVLLLASLVYMAGLFAIAWWGDKTKVYPENPRYRTLI
                     YAFALGVYGSSWTFYGAAGTAKQDGLLYIAGYFGPLVLLAFGIKFYERLVRLAKHQNA
                     TSISDFLSARFGRSRRIAVLVTIIALTAIIPYIALQLTAISMSIDVLTGKLPGVTPHW
                     YQDTTLGVALMLALFAILFGTRGIDATEHHPGMVLAIAAESVFKLLALVAVSVFALLS
                     LDSSVPLVTALRHIPEEFDQPSMFFAQALTAMFAFFCLPRQFHMGVVECADVADVRRA
                     RWWFGGYLVLLSIVVIPIVAASLPLDAAGISSPSNDALVLWIPLAAGHEWLALFAYLG
                     GYAAATGMVIVASVALSTMISNDLVLPMLWRWRLTALGKSVSSPAMILWVRRVAILCV
                     LLGAYGFFHLSPNAPSLAAIGTLSLAAVAQFAPAIIAAVYWPRASRAGVTGGLAAGFV
                     IWMYALLIPAIVSDGGAPHWVTYGPFGWSWLAPRSFLGLEFPDPFSHGVFWSLTVNTA
                     VMVMLSLRYQPAIGERLRVAGARLLEGFPGSRGGQLLPGGATVGDLLVLAERLLGAPA
                     ARRLLDRRSRELKRHVFAEERADLALLQSLERDLSGALGASSARLVLTSALRGAGIEL
                     AEIVTLFDEASQKLRFNRELLETMMDNMPQGVCVVNSDMHLVAWNQRYLDLFEYPPGF
                     VFAGKPVEELIRFNARRGWCGPGDLEAQVRKRLAYMRAGSIHTSERRRSDGRVIELRG
                     QPLPDGGFVTTFSDVTSHKRNEESLREINETLELRVEERTRQLAAAVAQAEQANQSKT
                     RFVVAASHDLLQPLGAARLFNAALRARAGQDGDLRGLAESVDNSLNAAGELLEGLLDI
                     SRLDAGGVRAEITEFDVGDLLNSLQKQFAPIAAQRGIRLAFKQTRLRVRSDPRLLRRV
                     LQNFIGNALRYTQSGSVVLGARRRSDGSTVKLHVIDTGPGITPENRVAIFEEFRRLDQ
                     PSPWGEKGLGLGLSICERIAAILDSTLTLESTPGRGSIFGIRVPRTGAGAAASFSELT
                     EHRIGRAEDASLSARILCVDDDAATLDGLRELLARWGFRVLAVASPEAAVALGADQSI
                     DIVLADYHLQNRPAGLELLNRLVNHAPAGQRRAGALLTADATEKLVLEADKLGFPVLR
                     KPVRPAALRALVAALVDRVARSNHASSSGAGGAN"
     gene            complement(3722..4405)
                     /locus_tag="VIV63_11080"
     CDS             complement(3722..4405)
                     /locus_tag="VIV63_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6125184.1"
                     /translation="MSPAILIADDHPLFREALKLAITQAFSGARTFEADTVDSLLATL
                     HAHPEIDLLLLDLGMPGAHGFSGLIQVRAHYPSVPVVVISGREDQDIAQRTLTHGAAA
                     FIAKSAAVPTIIDALQTILQGGVWTAPDLVPAAGARNHSVHPDEAAAARRVASLTPQQ
                     FRVLSMLCSGQSNKRIALQLQVSEATVKAHMTAILEKMGAENRTQAVLIAQRLALHQF
                     APPAPEDEA"
BASE COUNT          670 a         1455 c         1480 g          800 t
ORIGIN      
        1 agcaccgcgg tgtacatgtc ctggttgccg acgcgcgaat aagcgccgtg cccgcgcgcg
       61 aaggggatgc gcatgccacc gaccacggcg acacgacgaa cgttggatcc aaccagcatg
      121 gagagctccc ggcgaaaagc gcgcgctagg atgccacgtc aagaatacgt tcgggaacac
      181 gcgcgcaaaa attggactat cgtctgctca actaaatgac gacgaccgtt tcaggctggg
      241 tcttgctgct ggcgtcgctg gtctacatgg ccggcctctt tgccattgcg tggtggggcg
      301 acaagaccaa ggtttatccc gagaatcccc gataccgcac gctcatctac gcgttcgctc
      361 tcggtgtata cggatcgagc tggacgttct acggcgcggc gggcaccgcc aaacaagatg
      421 gtttgttgta catcgccgga tacttcgggc cgctggtgct gctggcgttc ggcatcaagt
      481 tctacgaacg cctcgtgagg ctcgccaaac accagaacgc cacgtccatt tccgatttcc
      541 tgtcggcgcg cttcggccgc tcgcgccgca tcgcggtgct ggtcacgatc atcgcgctca
      601 ccgcgatcat tccgtacatt gcactgcaac tgactgccat ctcgatgagc atcgacgtgc
      661 tcaccggcaa actgccgggc gtgacgccgc actggtacca ggacaccacg ctcggtgtcg
      721 cgctcatgct cgcgctcttc gcgatcctgt tcggcacgcg cggcatcgac gccaccgaac
      781 atcacccggg catggtgctg gccatcgccg ccgagtctgt cttcaaactg ctggcgctgg
      841 tggcggtcag cgtgttcgcg ctcctgtcgc tcgacagctc cgtgccgctc gtcaccgcgc
      901 tgcgccacat accggaagaa ttcgatcagc catcgatgtt cttcgcgcag gcgctgacgg
      961 cgatgttcgc gttcttctgt ctgccgcgtc agttccacat gggcgtcgtc gaatgcgccg
     1021 atgtcgccga cgtccgccgc gcacgctggt ggttcggcgg ctatctggtg ctgctcagca
     1081 tcgtggtcat tcccatcgtc gcggcgtccc tgccgctcga cgccgcgggc atcagttcac
     1141 ccagcaacga cgcgctggtg ttgtggattc cgctcgcggc ggggcacgag tggctcgcgc
     1201 tgttcgccta tctgggcgga tacgccgcgg ccaccggcat ggtcatcgtg gcgagcgtgg
     1261 ccttgtcgac catgatcagc aacgatctcg tgctgccgat gttgtggcgc tggcgcctga
     1321 ccgcgctggg caagagcgtg tcctcgccgg cgatgatcct ctgggtccgc cgcgtcgcca
     1381 tcctctgtgt gttgttaggc gcgtacggct tcttccacct gtcgcccaac gcaccgagcc
     1441 tcgccgccat cggcacgttg tcgctggcgg cggtcgcgca gttcgcgccg gccatcatcg
     1501 cggcggtgta ctggccgcga gcgagccgcg ccggggtcac cggtggcctg gcggccggct
     1561 tcgtcatctg gatgtacgcg ctgctgatcc ccgccatcgt ctccgacggc ggcgcgccgc
     1621 attgggtgac gtacgggccg ttcggatggt cgtggctggc gccgcgttcg ttcctgggtc
     1681 tcgagttccc ggaccctttc tcgcatggcg tgttctggtc gctcacggtc aacacagccg
     1741 tcatggtgat gttgtcgttg cgctatcagc cggcgatcgg cgagcggctg cgggtcgctg
     1801 gcgcgcggct gctcgagggg ttcccgggct cgcgtggcgg acagcttttg ccgggtggcg
     1861 ccaccgtcgg cgatcttctg gtgcttgcgg aaagattgtt aggcgctccg gcggcgcggc
     1921 gcctcctcga tcggcgctcg cgcgagctca agcgacacgt gttcgccgag gaacgcgcgg
     1981 acctcgcgtt gctgcagtcg ctcgagcgcg atctgtcggg tgcgctcggc gcgtcgtcgg
     2041 cccgcctggt gctgaccagc gcgctgcggg gcgccggcat cgaactcgcg gagatcgtca
     2101 cgctgttcga cgaagcttca cagaagctgc gcttcaatcg cgagctgctc gaaaccatga
     2161 tggacaacat gccacagggc gtctgcgtgg tgaactccga catgcacctc gtggcctgga
     2221 atcagcgcta cctcgatctg ttcgaatacc cgccgggctt cgtgttcgcc ggcaagccgg
     2281 tcgaagaact gatccgtttc aatgcgcgcc gcggttggtg cggacctgga gacctggagg
     2341 cgcaggtgcg caagcggctc gcctacatgc gcgcgggttc catccacacc tcggagcggc
     2401 gccgttccga cggccgcgtg atcgagcttc gcgggcaacc gctgccggat ggcggcttcg
     2461 tcaccacgtt cagcgacgtc acgagccaca agcgcaatga ggaaagcctg cgcgagatca
     2521 acgagacgct cgagctgcgc gtcgaggaac gcacgcgaca gctggcagcg gcggtggcgc
     2581 aggcggagca ggccaaccag tcgaagacac gtttcgtcgt cgccgccagc cacgacctgc
     2641 tgcaaccgct cggcgccgca cggctgttca acgccgcgct gcgcgcccgc gccggccagg
     2701 acggcgatct gcggggtctc gccgagagcg tcgacaattc gctgaacgcg gccggggagt
     2761 tgctcgaagg actgctcgat atctcgcgcc tcgacgccgg tggcgtgcgc gccgagatca
     2821 cggagttcga cgtcggcgac ctgctgaact cgctgcagaa gcagttcgcg cccatcgcgg
     2881 cgcaacgcgg aatccggctc gcgttcaagc agacccggct gcgcgtgcgc tccgatccgc
     2941 ggctgttgcg gcgagtgctt cagaacttca tcggcaacgc gttgcgttac acgcagtccg
     3001 gcagcgtggt gctcggcgcc cgtcgccgct ccgacggatc gactgtcaag ctgcatgtca
     3061 tcgacaccgg tccgggcatc acgcccgaga atcgcgtcgc catcttcgaa gaattccgca
     3121 ggctcgatca gccttcgccg tggggtgaaa agggattggg gctgggcctg tcgatctgcg
     3181 aacgtatcgc cgccattctc gacagcacgc tcacgctcga atccacgccc ggacgcggca
     3241 gcatcttcgg cattcgcgtg ccacgcactg gcgcgggtgc cgcggcgagt ttcagcgagc
     3301 tcaccgaaca ccggatcgga cgcgcggaag acgcatccct gagcgcgcga attctctgcg
     3361 tggacgacga cgcggcgaca ctcgacggtt tgcgcgaact gctggcgcgc tgggggtttc
     3421 gcgtgctcgc cgtcgcgtct ccggaggcag cggtggcgct cggcgccgat cagtcgatcg
     3481 acatcgtgct cgccgactat cacctgcaga accggcccgc gggcctcgag ctgttaaaca
     3541 ggctggtgaa tcacgcgccg gccggccagc gccgcgcggg cgcgctgttg accgccgatg
     3601 ccacggagaa actggtgctc gaggccgaca agctcggatt cccggtgctg cgcaaacccg
     3661 tgcggcccgc cgcgttgcgc gcgctggtcg cggcgctggt ggaccgggtc gcgcgttcga
     3721 atcatgcctc gtcttcggga gccggcggcg cgaactgatg cagcgcgagg cgctgcgcga
     3781 tcaacacggc ctgggtgcga ttctccgcgc ccattttttc gaggatcgcc gtcatgtgcg
     3841 ccttcaccgt ggcttctgac acctgcagct ggagcgcgat gcgtttgttc gactgcccgg
     3901 agcacaacat ggacaacacg cggaactgct gaggcgtgag gctggccacg cggcgcgcgg
     3961 cggcggcttc atcggggtga acactgtggt ttcgcgcgcc ggcggccggc accaggtccg
     4021 gcgcggtcca cacgccgccc tgcaggatgg tctgcagcgc gtcgatgatc gtcggcaccg
     4081 ccgccgactt ggcgatgaac gccgcggcgc cgtgcgtcag cgttcgttgc gcgatgtcct
     4141 ggtcttcgcg acccgagatc accacgaccg gaaccgatgg atagtgagcg cgaacctgga
     4201 tgaggccgga aaacccgtgc gcgcccggca tgccgaggtc gagcagcagc agatcgattt
     4261 ccggatgcgc atgcagtgtg gcgagcaacg agtcgacggt gtcggcttcg aacgtgcgcg
     4321 cgccactgaa ggcctgggtg atggcgagct tgagggcttc gcgaaacagc gggtgatcgt
     4381 cggcgatgag gatggcgggt gacat
//
LOCUS       DATDEU010000462         2484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_963043, whole genome shotgun sequence.
ACCESSION   DATDEU010000462 DATDEU010000000
VERSION     DATDEU010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963043"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VIV63_11085"
     CDS             <1..536
                     /locus_tag="VIV63_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY6125185.1"
                     /translation="ARAFEVLAHAPPAINANARLEMLRVLADAIGTRGMAGGQAIDLE
                     SVKQTLSEAALERMHRQKTGALIQASVLLGAISAGLTDAPERAALAEFGAEIGLAFQI
                     QDDILDVEGTTSSLGKRAGADADRVKPTYPSVMGLEKSRIQAFARRDRAIAALEPWGA
                     RFASLVEFANFLVARAS"
     gene            563..2461
                     /gene="dxs"
                     /locus_tag="VIV63_11090"
     CDS             563..2461
                     /gene="dxs"
                     /locus_tag="VIV63_11090"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY6125186.1"
                     /translation="MPNDSPSFALLRSLNSPADLRAHPENRLGEICDELRDYLIKTVA
                     TRGGHFAAGLGTVELTVALHYVFATPHDRLVWDVGHQAYPHKVLTGRRDRLDTIKQSG
                     GLAPFPSRAESEYDTFGVGHSSTSISAALGMAVAAELAKEDRKVVAIIGDGAMTAGMA
                     WEALFNAGARKTNLLVVLNDNDMSISENVGALSNYFAKVLSGRMYSTLRSGGKKILKQ
                     MPTMWELARRSEEMMKGMVLPGTLFEEFGFNFIGPIDGHDIKALVTNLRNMRNLNGPQ
                     LLHVVTKKGKGYDPAEKDPIKWHGPGPFDPVSGTIFKEKATGPTYSQVFGQWLCDMAA
                     RDERIVGITPAMREGSGMVEYSKKFPQRYFDVAIAEQHAVTFAAGLACEGWKPVVAIY
                     STFLQRAYDQLIHDVALQNLPVVFALDRAGLVGSDGATHQGSYDYSFLRCIPNLVVMA
                     PADENECRQMLYTATTLNAPAAVRYPRGQGPGVAPVAEMTALPLGRAEIRREGASGLL
                     ILAFGALVRSAEIIGERLDATVVNMRFVKPLDEELVLRLAAKHNALVTIEENAVAGGA
                     GSAVLELLANCATLLPSLTLGIPDQFIEHGSRENCLAEAELDQASLSAAVERWWSPLA
                     GKQARSGT"
BASE COUNT          451 a          845 c          785 g          403 t
ORIGIN      
        1 aggcgcgcgc attcgaagtg ctggcgcacg cgccgcccgc gatcaacgcg aatgcgcggc
       61 tcgagatgct gcgcgtgctc gccgacgcca tcggaacacg cggcatggcc ggcggccagg
      121 cgatcgacct cgaatccgtc aaacaaacgc tgagcgaggc tgcgctcgaa cgcatgcacc
      181 ggcagaagac cggcgcgctc attcaggcca gcgtactgct cggcgcgatt tctgccgggc
      241 tcaccgacgc gccggaacgc gcggctctcg cggagttcgg cgcggaaatc gggctcgcgt
      301 ttcagatcca ggacgacatc ctcgacgtgg aaggcaccac gagttcgctc ggcaaacgcg
      361 cgggagccga tgcggatcgc gtcaagccga catatccgtc cgtcatgggc ctcgaaaaat
      421 cgcgtatcca ggcattcgcg cgccgcgatc gcgcgattgc ggcgctcgag ccgtggggcg
      481 cgcgctttgc gtcgctcgtg gaattcgcga atttcctggt ggcgcgcgcc agctgagttg
      541 gcgtgtaaac ttcgccccga acatgcccaa cgattcgcca tcattcgcgc tgctgcgctc
      601 gctcaattcg ccggcggatc tgcgcgctca tcccgaaaac cgtctcggcg aaatctgcga
      661 cgagctgcgc gactacctca tcaagaccgt cgcgacgcgc ggcggccatt tcgccgccgg
      721 gctcggcacc gtcgaactca ccgtcgcgtt gcactacgta ttcgccacgc cacatgaccg
      781 gctggtgtgg gacgtgggcc accaggctta tccgcacaag gtgctcaccg ggcggcgcga
      841 ccgcctcgac accatcaagc aaagcggcgg ccttgcgccg tttccgtcgc gcgccgagag
      901 cgaatacgac actttcggtg tcggtcactc gagcacttcg atcagcgccg cgctcggcat
      961 ggccgtcgcc gcggagctcg ccaaagaaga tcgcaaggtc gtcgcgatca tcggcgacgg
     1021 cgccatgacc gccggcatgg cctgggaagc gttgttcaac gccggcgcgc gcaagaccaa
     1081 cctgctggtc gtgctcaacg acaacgacat gtcgatctcc gagaacgtcg gcgcgttgtc
     1141 aaactacttc gccaaggtgc tgtcggggcg catgtactcc acgctgcgct cgggcggcaa
     1201 gaaaatcctc aagcagatgc ccaccatgtg ggaactcgcc cgccgctcgg aagagatgat
     1261 gaagggcatg gtgctgcccg gcacgttgtt cgaggaattc ggattcaatt tcatcgggcc
     1321 catcgacgga cacgacatca aggcgctggt cacgaacctg cgcaacatgc gcaacctcaa
     1381 cgggccgcag ctcctgcacg tggtcacgaa gaagggcaag ggttacgacc cggccgagaa
     1441 ggacccgatc aagtggcacg gcccgggtcc gttcgatccg gtcagcggca ccatcttcaa
     1501 ggagaaggcc accggcccca cctactcgca ggtgttcggg caatggctgt gcgacatggc
     1561 cgcgcgcgat gaacgcatcg tcggcataac cccggccatg cgtgaaggct cgggcatggt
     1621 cgagtactcg aagaaatttc cgcagcgcta tttcgacgtc gccatcgccg aacaacacgc
     1681 cgtcacgttt gccgccgggc tcgcctgcga aggctggaag cccgtggtcg ctatctactc
     1741 cacgttcctg cagcgcgcct acgaccagtt gatccacgac gtggcgttgc agaacctgcc
     1801 ggtggtgttc gcgctcgatc gcgccgggct cgtcggcagc gacggcgcca cgcaccaggg
     1861 ttcgtacgac tactcgttcc tgcgctgcat tccgaacctc gtcgtcatgg cgcccgccga
     1921 cgaaaacgag tgccgccaga tgttgtacac cgcaaccacc ctcaacgcgc cggcggccgt
     1981 gcgttatcca cgcggccagg gtccgggcgt cgcgcccgtc gccgagatga cggcactgcc
     2041 actcggccgc gcggagatcc ggcgtgaagg cgcgagcggc ctgctgatac tcgcgttcgg
     2101 cgcgctggtg cgttcggccg agatcatcgg cgagcggctc gacgccaccg tggtcaacat
     2161 gcggttcgtc aaaccactcg acgaagagct ggtgttgagg ctcgcggcca aacataacgc
     2221 gctcgtcacc atcgaagaaa acgccgtcgc cggcggcgcc ggttccgcgg tgctcgaatt
     2281 gctggccaat tgcgcgacct tgctaccttc cctcacgctc ggcattcccg accagttcat
     2341 cgaacacggc tcgcgcgaaa actgcctcgc ggaagcggag ctggaccagg ccagtctctc
     2401 cgccgcggtg gagcgctggt ggagtccgct ggccggcaag caggcgcgtt ccgggacctg
     2461 aaacaggcca ccggccgcgc aatt
//
LOCUS       DATDEU010000463         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1983917, whole genome shotgun sequence.
ACCESSION   DATDEU010000463 DATDEU010000000
VERSION     DATDEU010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983917"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VIV63_11095"
     CDS             complement(<1..502)
                     /locus_tag="VIV63_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY6125187.1"
                     /translation="MSPETGRRLTLIRHANAEQDVDVRDFERPLSRKGQNEALEMARR
                     FQERGLVPDLILASAATRTRETAETFAKVLGVASRLLQADDALYLAEGDYILGAIRAV
                     GPRVNHLMVIGHNPGISAAAISLAPEAITNDLPTCGTLTMAVSCATWNLIDRRCVRDS
                     ERDSPRK"
     gene            complement(499..807)
                     /locus_tag="VIV63_11100"
     CDS             complement(499..807)
                     /locus_tag="VIV63_11100"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY6125188.1"
                     /translation="MTVWTDIGSLAELESSSRIEGDVDGYRVRVARVSGMLYAFEDRC
                     THDDSPFDDAPIEDTQIICPRHGARFCLRTGAALSPPAYEPLRIFEVRENAGRIEVLA
                     "
     gene            complement(814..1365)
                     /gene="sufT"
                     /locus_tag="VIV63_11105"
     CDS             complement(814..1365)
                     /gene="sufT"
                     /locus_tag="VIV63_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEY6125189.1"
                     /translation="MRTHENEPFVLRRDVKAVMVPAGIDIELKSGQSGFITQALGGSF
                     TLYMEGNLFRMAGTDADAIGKEVAKAPELPPDSTEDDVLALAWEQLRTCYDPEIPINI
                     VDLGLIYECTVSAKPEGGRVVHVRLTLTAPGCGMGDVLVQDIRQKLEIIPTVVSADVE
                     LVFDPPWNQSMMTEAARLQTGMF"
     gene            complement(1368..1811)
                     /locus_tag="VIV63_11110"
     CDS             complement(1368..1811)
                     /locus_tag="VIV63_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY6125190.1"
                     /translation="MDLKDLYRDVILDHNKTPRLFGRLEPADSSADGHNPLCGDRLHV
                     TVKLDGDRVSDLRFDGKGCAISVASASLMCEAVKGKRRSEITHLFNEVHEVLTKHDAP
                     IPDDLGKLAALSGVREFPVRVKCASLCWHTLNAALDRSVEPVKTE"
     gene            complement(1811..>2104)
                     /locus_tag="VIV63_11115"
     CDS             complement(1811..>2104)
                     /locus_tag="VIV63_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6125191.1"
                     /translation="EYSTARLQAIPGLHIVGTAVHKAAVVSFTVDGIHPHDLGTILDH
                     EGVAVRTGHHCAQPVMEFFGVPATARASFACYSRESDVDALVDALAKAREVFG"
BASE COUNT          359 a          663 c          690 g          392 t
ORIGIN      
        1 acttcctcgg ggagtcgcgc tcggagtcgc gcacgcatcg ccggtcgatg agattccagg
       61 tggcgcaact caccgccatg gtcaacgtgc cgcaggtcgg cagatcgttg gtgatggcct
      121 cgggcgcgag cgagatggcc gccgcggaga tgccggggtt gtggccgatc accatgaggt
      181 gattgacgcg cggtccgacc gcgcggatgg cgccgagtat gtagtcgccc tcggccaggt
      241 agagcgcatc gtcggcctgc aacaaccgcg aggccacgcc taacaccttt gcaaaggtct
      301 cggccgtttc gcgcgtgcgc gtcgcggcgc tggcgaggat gagatcggga accaggcctc
      361 gctcctgaaa gcgtcgcgcc atctccagcg cttcgttctg gcccttgcgg ctgagcgggc
      421 gctcgaaatc ccgcacgtcc acatcctgct ccgcgttggc gtgccggatc agcgtgaggc
      481 gccggcccgt ttcagggctc acgcaagtac ctcgattcgt cccgcgttct cgcgcacctc
      541 gaagatccgc aagggttcat acgcaggcgg ggagagcgcc gcacccgtgc gcaggcagaa
      601 gcgcgcgcca tggcgcgggc agatgatctg ggtgtcttcg atgggagcat cgtcaaacgg
      661 tgagtcgtcg tgagtgcaac ggtcttcgaa tgcgtagagc atcccactta cccgggccac
      721 ccgcacgcgg tagccgtcga catcgccctc gatacgtgaa ctggattcga gttccgcgag
      781 gcttccgatg tcggtccaaa cggtcatatt ttgttagaac atccccgtct gcagccgggc
      841 ggcctcggtc atcatcgact ggttccacgg cgggtcgaac accagttcga cgtcggccga
      901 tacgacggtg gggatgattt ccagcttctg ccggatgtcc tggaccagca cgtcgcccat
      961 gccgcagccc ggcgccgtca gcgtgagccg cacgtgaacc acgcggccac cctcgggttt
     1021 ggcgctgacc gtgcattcgt agatgaggcc gaggtcgacg atgttgatgg ggatctcggg
     1081 gtcataacag gttcgaagct gctcccaagc cagcgccagg acgtcgtctt cggtggaatc
     1141 gggcggcaat tccggcgcct tcgccacttc cttgccaatg gcgtcggcat cggttcccgc
     1201 catgcggaac aggttgcctt ccatgtaaag cgtaaagctt ccgcccagtg cctgcgtgat
     1261 gaaaccggac tgaccggact tgagttcgat atcgattcca gcggggacca tcaccgcttt
     1321 gacatcgcgg cgaaggacga acggctcgtt ttcgtgggtt ctcatcgtta ttcagtcttc
     1381 acgggttcga cactgcgatc gagtgcggcg ttcagcgtgt gccagcacag gctggcgcat
     1441 ttcacgcgca ccgggaattc gcgcacaccc gacagcgcgg cgagtttgcc caggtcgtcg
     1501 gggatgggcg catcgtgctt cgtcagcact tcgtgcacct cgttgaacag gtgcgtgatc
     1561 tccgagcggc gtttgccctt gaccgcttcg cacatcaacg aggcggaggc cacggagatg
     1621 gcgcagcctt tgccgtcgaa ccgaagatcg gagacgcgat cgccgtcgag cttcacggtg
     1681 acatgcagtc gatcgccaca cagcggattg tggccatcgg ccgacgagtc ggcgggctcg
     1741 aggcgcccga acaggcgcgg cgtcttgttg tgatccagga tcacgtcgcg atagaggtcc
     1801 ttgaggtcca tcagccgaac acctcgcgtg ctttcgcgag cgcgtcgacc agcgcatcga
     1861 cgtcggactc gcgcgagtaa cacgcgaacg atgcgcgcgc cgtggcgggc accccgaaga
     1921 attccatgac gggttgggcg caatggtgcc cggtgcgtac ggccacgcct tcgtgatcga
     1981 gaatcgtgcc caggtcgtgc gggtggatgc catcgaccgt aaacgacacc actgccgcct
     2041 tgtgcaccgc ggtgccgacg atgtgcaggc ccggaatggc ctgcagccgt gccgtcgagt
     2101 actc
//
LOCUS       DATDEU010000464         9928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1546705, whole genome shotgun sequence.
ACCESSION   DATDEU010000464 DATDEU010000000
VERSION     DATDEU010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546705"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1690
                     /locus_tag="VIV63_11120"
     CDS             26..1690
                     /locus_tag="VIV63_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6125192.1"
                     /translation="MRPVFGATRRSVLQALAGGAGLLAAGCNQKEKAAIAAPPKPETA
                     ASRDVPFLAPFTINKAKSLGVECIDVHAHFFNASDVPIEGFLEGPVAHSMPNGFLAAL
                     VRALAPIADGLGVFAITAKQEHEKLLGMGPGFREFTPQRAHEELNALQQTERRAISEE
                     FYRLTQTPEGKQFREEYRRLMSAAPATESINGSPRVRDIEKDSVFRAMYMTEQPASEA
                     QLIEMEAVSEETFGEGVLAFVGYMMSARWSNLQTYKRAFSTSSESVGVNQVLGALVNF
                     DGWLDGKLRSPHRDQMELHALMSRLSGGYMRPIMSYNPWPDAMNAGESLALLRDAKKL
                     DFVGAKIYPPNGFRPWGNAGKPPDRRGGPTGDQIDQALEAFWIQCHEWNFPVMAHAGP
                     SMGRDDAHDFLSGPNHWGDLLNAKFWTDDSGPRVNLGHFGGETNHDKAAGKDWPDKFG
                     SEMGKKRGEFLFADLGYWDGLQCPKPGKKKCVDARARLANALKTPVGTGTVADRVMFG
                     SDWLMLSKEKNWPSYARQLFKSVQAVAPASAAAIFSGNARRLFTAL"
     gene            1752..4496
                     /locus_tag="VIV63_11125"
     CDS             1752..4496
                     /locus_tag="VIV63_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY6125193.1"
                     /translation="MKRDPAELKLLSKLLDDALALSPAERETWIENLPATLDGLKPTL
                     RKLLFTTGPETADMVRFGQHVSAALEGTLAAAANLRAGSAVGAYELIRELGRGGMGVV
                     WLARRTEGLTNRQVALKLPHVDFFNARLAERLARERDILESLTHPNIARLYDAGVTST
                     GQPFLALEYIEGIPLTAYCDKQSMSVRDRLGLFVQVLQAIQYAHSNLVIHRDLKPANI
                     LVTDKGTAVVLDFGIAKLLTDAAPAENAMTEFGNRALTPDYASPEQILGKPISTASDL
                     YSLGVLLSQLLCGSRPYSLKRDSRGALEDAITEAEPRVPSRNVTPEAAASRGVSASAL
                     VRELRGDLDNIILKALRKDPSDRYASVAQFAQDLDRHLKGEAVLAHPGSARYRAGKFL
                     RRHTLAVASAATILVLLILGTAAVAWQAHEARLEAARADQVKRFALSLIESADTANGA
                     GVATTAVELLQKARQRVETELADRPAIAAELMSAIGSGLLGQGRPEDAAELLRKSAAL
                     SASVNGAEDIRTLDTQILQAEALVGLGKTEEAIVLLKPVIDIAHRQHANLTEADGWRW
                     LSMAQIEQGDFDAGIASAQAAVTAVGPAPAGQSALLGATLANLSLASALNSDRRPGGT
                     DAARAALLYASTPGVPGNTPPGSQARALLGEGLIRDGEVAAGLREIEQAYNDSRALLG
                     PDHPQTVQMGNLLGGGRLEAGDVKGAVAAYRVCYESAQRHPAALSTIAFAYVNLTLAN
                     ALVAARDGESALPHYIEAERLFAEADGPTAPMTLGVRSSRGLALARLGRLQEADDVFA
                     SLEDKTLTGRGKAMYESRLAVLRSLQGRHVEAVALAVPATEGLSKMASKMLRAQSLSR
                     LGSVLLAAGQRAEAVAPLEQAMALYNTQLQESPDRTETAADLRLATVSKQQPESRIPP
                     "
     gene            4523..6154
                     /locus_tag="VIV63_11130"
     CDS             4523..6154
                     /locus_tag="VIV63_11130"
                     /inference="COORDINATES: protein motif:HMM:NF025870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tachylectin-related carbohydrate-binding
                     protein"
                     /protein_id="HEY6125194.1"
                     /translation="MRRSTWMACLALLFASMARNASADSYLPPIGGAGGGQFIGACKP
                     SEMLTGFQLRIIDGGRGSVPGVGSFEGLSGYLDALRPVCAVATSATRIGTPTLAGSWG
                     GVAAGDQAAWQSAGFFGGTATVSNNAQLINRLCPVDQPLVSGVTVNYGKRGGFLVVYS
                     VHLSCGLAAAEQGLSVSDAGLAVRANQRATRSAPDSSAADAAALAAIYGESRKQSCPR
                     GPAAIGAHGRAGALVDAVGLICGAPRVTPASKPPVISARTSASSNLLSSLNAGQATPQ
                     PQPPSPALDAANPAIIYGTGADGILRWYRHNGATNGVGANIAGSWQSADNVSSDWGGF
                     KQIFTGGNGVIYGITQDGKLLWYRHAAFLTGESADAPGAWFGAREVGGGWGGYRHAFS
                     AGDGIIYAITEDGQLLWFRHTGFADGRAEWEGPIPLTGGWSNYEQVFSGGAGVIYAIG
                     ADGILKWNRHDGYQSGANDWRAAKDVGRGWNGFRSVFSAGNGIIYAIAPDGLLRWYRH
                     NGYLDGRGLESPNAWQGRTDVGHGWNSFVSVVAQF"
     gene            6203..7045
                     /locus_tag="VIV63_11135"
     CDS             6203..7045
                     /locus_tag="VIV63_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007940144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HEY6125195.1"
                     /translation="MKTPPGLQPLIDDGVIDEVLRSLKSGKEATVYMVRTGEQTRCAK
                     VYRDMGQRSFQKRAQYQEGRKVRGSRQARAMAKSTRYGRKEAESAWKNTEVDALYKLV
                     EAGVRVPRPFGYFNDTLVMELVTDAAGDPAPRLGEVDLTPEVAREYHAFLIRQIVRML
                     NIGLIHGDLSEFNVLVDAHGPVIIDLPQAVNAAGNNAAFAMLERDVNNIRGTLGRFAP
                     ELLNTEYAREIWALYEAGELQAESVLTGVFAREEGVTDPDAVMVVIDDAREENIRREL
                     GRLA"
     gene            7088..>9928
                     /locus_tag="VIV63_11140"
     CDS             7088..>9928
                     /locus_tag="VIV63_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6125196.1"
                     /translation="MNSRIWLPALGALLACACTKQPSVPNGTLIVDEDVALTRQADRP
                     VDTATRELTATDDAILVAIVDENLTNVKLRLSTTDTGGDAPAPVEVENNLSGSGLELA
                     ALEVSEGARIRVTLTGPQDSVQPGKVHIRIRQYAPGAMRDPKFSQQLGAFKAWTAATN
                     ASFRADKARETGLADIQHAIDGLESTHGDATLAAEARLIKARMLYFFRVDWREARAEA
                     QRAAAAFAHLPTPDALNEARAKYIEALALGEISNDRESKDPTPEEALRLSTEMIQSLS
                     ADTSALGPIDRARAMGSLARLDVKAMNAEGANKHFGLAREMFQAAGYTAGVREMRWSL
                     GLVFVELGMFADAAREFDVLLPDIDTISDPELHVRAYLAVGRAQTFSDRTDEGAQMLL
                     KALPLAQQYQLRQLEVMALMGLGYHYQNRGDMLQMGVFFDEALKIAREQKDVKEYVEA
                     LTAAATAARDTGNLDRAFELDKEAVRKSPTSVLECRTKMSLGLDYYRAQDLPSAIEWY
                     RKSLAVDLNDPMSHIYTDGKLGLALFLIEHAPSTAKELAEADKLLAEAQETSTKVRDQ
                     WRVIIALRIRAALEARRGQNDAALAHLDQVFTLSQEYRQRSKSTEARSGMLRAEEHGF
                     RGYLDIIFADVAKRGPGVIRAATPRELAGLRRLERARYDSFGGLRVSAPDAKTSARVD
                     QIFSEMGQKSLKIAGLLKSNLDAAQKAELQGLQSDMARLHAELDNVRTVAADKQASGA
                     SPPDARRNFRALAAGAAQLSYALGANHVYAVVRSETGTVVTVLAPSRKDLEAQLGDFS
                     KFEVQTASREIEAALEEISSVLLPAGLLPEKSSTVEIVAEGRIGNVPFPALRSPTNAQ
                     RRLAETHVVSMIMSLFDQDDTLRAQHARPYRFVALASGTGTYRAAVVDPTPRLQAATK
                     EIRVAADLFTARDSTAKIKLFTGDDGTATALRD"
BASE COUNT         1815 a         3157 c         3333 g         1623 t
ORIGIN      
        1 aagaacaatg atttcgggag ggcgtatgcg gccagtgttc ggtgcgacca ggcgatcggt
       61 ccttcaggcg ctcgccggcg gtgcgggcct gttggctgcg ggatgcaatc aaaaggagaa
      121 ggccgccatc gccgcgcctc ccaaacccga aaccgcggcg tcgcgggacg ttcccttcct
      181 ggcgccattc accatcaaca aggcgaagtc gttgggcgtc gaatgcatcg acgtccacgc
      241 gcacttcttc aacgcctcgg acgtgccgat cgagggcttt ctcgaaggtc ccgtcgccca
      301 ctcgatgcct aacgggttcc tggcggcgct cgttcgcgcg ctggcgccca tcgccgacgg
      361 actgggcgtg ttcgcgatca cggcaaaaca ggaacacgag aagctgctgg gcatgggtcc
      421 cggctttcgc gagttcaccc cccagcgcgc acacgaagaa ttgaacgcct tgcagcaaac
      481 agaacgccgg gcgatctctg aggagttcta tcggctgacg cagacacccg agggcaagca
      541 attccgcgag gagtatcgcc gtctcatgag cgccgccccg gcgaccgaaa gcatcaacgg
      601 cagcccgcga gtccgcgaca tcgaaaagga ttcggttttc agggcgatgt acatgacgga
      661 gcagccggca agcgaagccc agctcatcga gatggaagcc gtgtcggaag aaacattcgg
      721 cgagggcgtg ctcgcgttcg tgggttacat gatgtccgcg cgctggtcca atctgcagac
      781 ctacaagcgc gcgttttcga cgagcagcga gagcgtcggc gtgaaccagg ttctcggcgc
      841 tctcgtgaac ttcgatggct ggctcgacgg caagctgcgc tcgccgcaca gagaccaaat
      901 ggagctgcac gcgctgatgt cgcgtctgtc cggtggatac atgcggccca tcatgtcgta
      961 caacccgtgg cccgacgcca tgaacgcggg cgaatcactg gccttgctga gggacgcgaa
     1021 gaaactggac ttcgtcggcg cgaaaatcta tccacccaac ggtttccgtc cgtggggcaa
     1081 cgccggcaaa ccaccggatc ggcgtggcgg gcccacgggc gatcagatcg atcaggcgct
     1141 cgaggctttc tggattcagt gccacgaatg gaattttccg gtgatggcgc acgcaggacc
     1201 gtcgatgggc cgcgacgacg cgcacgattt cctgagtggc cccaaccatt ggggcgacct
     1261 gctcaatgcg aagttctgga ccgacgacag cgggccgaga gtcaaccttg gccacttcgg
     1321 cggcgaaacg aaccacgaca aggccgcggg caaggactgg cctgacaagt tcggcagcga
     1381 gatgggcaag aagcggggtg agttcctgtt cgcggatctc gggtactggg atggcttgca
     1441 gtgtccgaaa cccggcaaga agaaatgcgt cgacgccagg gcacggctcg cgaacgcgct
     1501 gaagacgccg gttggcacgg gcacggtcgc cgaccgcgtg atgttcggca gcgactggct
     1561 catgttgtcg aaggagaaga actggccttc ctacgcgcgc cagttgttca agtcggtaca
     1621 ggccgtggcg ccggccagtg cggctgccat cttcagcggg aatgcgagaa ggcttttcac
     1681 ggcgctttga cccgataacg agcgccgccg gttatgctcg ggcagcttcc cgctgactcg
     1741 agggcatttc cttgaaacgc gatccggccg agctcaagct cctctcgaaa ctcctggacg
     1801 acgcgctggc gctctcgccc gcggaacgcg agacgtggat cgagaacctt cccgcgactc
     1861 tggacggtct gaaacccacg ctgcgcaagc tgctcttcac cacgggccct gaaacggcgg
     1921 acatggtccg cttcggccag catgtcagcg ccgcgctgga aggcacgctc gcggcagcgg
     1981 ccaatctgcg agcgggttcg gctgtgggtg cctacgaact cattcgcgaa ttgggccgcg
     2041 gcggcatggg cgtggtgtgg ctggcgcgcc gaacggaggg tctcaccaac cgacaggtgg
     2101 ctctcaaact accgcacgtc gacttcttca atgcgcggct cgcggagcgc ctcgcacgcg
     2161 agcgcgacat tctcgagagc ctgacgcacc cgaacatcgc ccggctctac gacgcgggcg
     2221 tgacgagcac cggtcagcct tttctcgcgc tcgaatacat cgagggcata ccgctcacgg
     2281 cgtactgcga caaacagtcg atgagcgtgc gcgatcgcct cgggttgttc gtgcaggttc
     2341 tgcaggcgat ccagtacgcg catagcaacc tggtgataca tcgcgatctc aagcccgcga
     2401 acatcctggt gaccgacaaa ggcacggctg tcgtcctcga tttcggcatc gccaagctgc
     2461 tgacggacgc ggcgcccgca gagaacgcga tgacggagtt cggcaatcgc gcgttgacgc
     2521 cggactacgc ctcgccggaa cagatactgg gcaagcccat cagcacggcg tcggatctct
     2581 attcactcgg tgtcctgctc tcccaactgc tgtgcggctc gcgaccgtac tcgctcaagc
     2641 gggattctcg cggtgcgctc gaggatgcca tcaccgaggc cgagccccgc gtaccgagcc
     2701 gcaatgtcac gccggaggcg gccgcgtcgc gcggcgtttc ggcgagcgcg ctcgtgcgcg
     2761 aactgcgcgg tgacctcgac aacatcattc tcaaggcgct gcgaaaggac ccgtcggatc
     2821 gttacgcgag cgtggcgcag ttcgcgcagg acctcgatcg ccatctcaag ggtgaagcgg
     2881 tactcgcgca tccaggaagc gcgcgatacc gagcgggaaa attcctgcgg cgtcacacgc
     2941 tggcggtggc gtcggccgcg acgattctcg tgttgctcat actgggaacc gcggcagtag
     3001 cttggcaggc gcacgaagcg cgtctcgaag cggcgcgcgc cgaccaggtg aagcgtttcg
     3061 cgttgtcgct catcgagagt gcggacacgg cgaacggcgc gggcgtagcc accacagcgg
     3121 tggagctgct gcagaaggca cggcaacgcg tcgagacgga gcttgccgac cggccggcga
     3181 tcgccgcgga actcatgtcg gccatcggct ctggtttgtt agggcaaggg cgccccgagg
     3241 acgcggcgga gcttttgaga aagtctgccg cgttgtcagc cagcgtcaat ggtgcggaag
     3301 acatccgcac gctcgacacg caaatcctcc aggccgaagc attggtcggt ctgggcaaga
     3361 cggaagaagc cattgtgctg ctcaaacccg tcatcgatat tgcacatcgc cagcacgcca
     3421 atctcaccga agccgatggc tggcgctggc tcagcatggc gcagatcgag cagggcgatt
     3481 tcgatgcggg cattgcgtcg gcgcaggcgg cggtgaccgc ggtgggcccc gcgcctgccg
     3541 gccagtccgc gctgctcggc gcgacactcg cgaacctgtc tctcgcgagt gcgctcaaca
     3601 gcgatcgccg tccgggtggg accgacgcgg cgcgcgccgc attgctttat gcgagcacgc
     3661 ctggcgttcc gggtaacacg ccgcccggat cgcaggcccg tgcgctgctc ggagagggct
     3721 tgatccgcga tggcgaagta gcggcggggc ttcgcgagat cgagcaggct tacaacgaca
     3781 gtcgagcgtt gctgggcccg gatcacccgc agacagtaca aatgggcaat ctactcggtg
     3841 gcgggcgact cgaagccggc gacgtgaaag gcgccgtggc ggcttatcga gtctgttacg
     3901 agtccgcgca acgccacccg gccgctttga gcacgatcgc gtttgcgtac gtgaatctga
     3961 cgctggccaa cgcactggtg gcggcgcgcg atggtgagag cgcactgcct cactacatcg
     4021 aggcggaaag gttgtttgct gaagcggatg gcccgaccgc gccgatgaca ttgggtgtgc
     4081 gctcgtcgcg tggcctcgcg ctggcgcggc ttggccgcct gcaggaagcc gacgatgttt
     4141 tcgcaagcct cgaggacaag acgctcaccg gcagaggcaa ggccatgtac gagagccggc
     4201 tggcggtcct gcgcagtctt caaggccggc acgtcgaagc cgtggcgctc gcggttcccg
     4261 caaccgaagg cctgagcaaa atggcttcga aaatgttgcg cgcgcagagc ctgtcgcggt
     4321 tggggtccgt gctgctggct gccggccagc gagccgaagc cgtggcgccg ctcgaacagg
     4381 cgatggcgct ctacaacacc caactgcagg aatcgccgga ccgcaccgag acggccgccg
     4441 acctgcgcct cgcgaccgtg tccaaacaac agcccgaatc gcgtatcccc ccatagagag
     4501 tgtctctttt ggggagctgc cgatgcgaag gtcgacctgg atggcgtgtc ttgcgctgct
     4561 gttcgcgagc atggcgcgca acgcgtccgc ggatagctac ctgccgccga taggcggcgc
     4621 gggcggtggc cagttcatcg gcgcctgcaa acccagcgag atgctcaccg ggttccagtt
     4681 acgcatcatc gacggcgggc gcggaagcgt tcccggcgtc ggttcgttcg aaggtttgtc
     4741 aggctatctc gatgcgttgc gtccggtctg tgccgtcgcc acttccgcga cgcgcatcgg
     4801 cacgccaaca ctcgcgggct cgtggggcgg ggtggccgca ggtgatcagg cggcgtggca
     4861 atcggctgga ttctttggcg gcacggcgac cgtctccaac aatgcgcaac tcatcaatcg
     4921 tctctgtccc gtggatcagc ccctcgtcag cggcgtcacg gtgaactacg gaaagcgcgg
     4981 cggcttcctg gttgtctaca gtgtgcacct gtcgtgcggg ctcgcggcgg cagagcaggg
     5041 tctgtcggtg agcgacgcgg gactcgcggt gcgcgcgaat caacgtgcga ccagaagtgc
     5101 gccggattcc tcggccgccg atgccgcggc gttggctgca atttatggtg agagccggaa
     5161 gcagtcgtgt ccgcgcggcc cggcggcgat cggcgcacac ggccgcgcag gtgcgctcgt
     5221 cgatgccgtg ggcctcatct gcggcgcgcc ccgtgtgacg ccggcatcga aaccgcccgt
     5281 gatttccgcg cggacctcgg cgagttccaa tctgctcagc tctctcaatg ccgggcaagc
     5341 cacgccgcaa ccgcaacccc cttcacccgc gctcgatgcc gcaaacccgg cgatcatcta
     5401 cggcactggt gccgatggca tcctgcgctg gtaccggcat aacggcgcta ccaacggagt
     5461 cggcgccaac atcgcgggct cgtggcagag cgctgacaat gtcagcagcg actggggcgg
     5521 gttcaaacag atattcacgg gcggcaatgg cgtgatctac ggcatcaccc aggacggcaa
     5581 gctgctctgg tatcgccacg ccgcgtttct cacgggcgag agcgccgacg cgcccggcgc
     5641 gtggtttggt gcgcgcgagg tgggcggcgg ctggggcgga tatcgccacg cattctcggc
     5701 gggcgatgga atcatctacg cgatcacgga agacggccag cttctgtggt tccgccacac
     5761 cggcttcgcc gatggccgtg cggagtggga aggtccaatc ccgctcaccg gcggctggtc
     5821 taactacgag caggtgttct cgggtggcgc cggcgtgatc tacgccatcg gtgcggatgg
     5881 catcctgaaa tggaatcgtc acgacggcta ccagagcggt gcgaacgact ggcgcgccgc
     5941 gaaggacgtc ggccgtggct ggaacggatt ccgatccgtg ttctccgcgg gaaacggcat
     6001 catctacgcc atcgcacccg acgggctgct gcgctggtat cgccacaacg gctacctgga
     6061 tggccgcggg ctcgagagtc cgaacgcatg gcaaggccgg accgatgtgg gtcacggatg
     6121 gaactcattc gtgagcgttg tggcccagtt ctgaggaaac ccgcgggttc ggtgtctccg
     6181 ggctgttaag ctgcgcgccc tcatgaaaac ccctcccggc ctccagccac tgatcgacga
     6241 cggcgtcatc gacgaagtcc tccgctccct caagtccggc aaggaagcca cggtctacat
     6301 ggtccgcacc ggcgagcaga cccgttgtgc caaggtctat cgcgacatgg gccagcgcag
     6361 tttccagaaa cgcgcgcagt accaggaagg gcgaaaggtg cgcggcagcc gccaggcacg
     6421 cgcgatggcg aagagcacgc gctacggccg caaggaagcg gagtccgcgt ggaagaacac
     6481 cgaggtggat gcgctctaca aactcgtcga ggcgggtgtg cgagtgccgc ggccgttcgg
     6541 ctacttcaac gacacgctgg tgatggagct ggtgaccgac gcggccggcg atccggcgcc
     6601 acggctcggt gaagtggacc tgactcccga ggtggcgcgc gaatatcacg cgttcctgat
     6661 tcgccagatc gtgcgcatgt tgaacatcgg cctgatccac ggcgatctct cggaattcaa
     6721 cgtgctggtc gacgctcacg gcccggtgat catcgatctg ccgcaggcgg tgaacgcggc
     6781 gggcaacaac gcggcgttcg cgatgctcga gcgcgacgtt aacaatatcc ggggcacgct
     6841 gggccgtttc gcgcccgagc tactgaatac cgagtacgcg cgcgagatct gggcgctgta
     6901 cgaagcgggc gaactgcagg cggaaagcgt tctcacgggc gtgttcgcac gagaagaggg
     6961 cgtcaccgat ccggatgcgg tgatggtggt catcgacgac gcgcgcgagg aaaacatccg
     7021 ccgcgagctg ggacggctcg cctagctttc gattaagatt ctccccggat ctggggaggt
     7081 ctcaaggatg aactcaagaa tatggctgcc ggcactcggc gcgctcctgg cttgcgcgtg
     7141 cacgaagcag ccctcggtgc ccaacggcac actgatcgtc gatgaagacg ttgcactgac
     7201 ccggcaggcc gaccggcctg tcgacacggc cacgcgtgag ctcacggcca cggacgacgc
     7261 cattctcgtt gcgatcgtcg atgagaacct gacgaacgtg aagctccggc tctcgacgac
     7321 ggataccggc ggcgatgcgc ccgcgccggt cgaagtcgaa aacaatctgt cgggctcggg
     7381 cctcgagctc gcggcgctcg aggtatcgga aggcgcacgc attcgcgtga cgctgacggg
     7441 tccccaggat tcggtgcagc cgggcaaggt gcacatccgc atccggcaat atgcgccggg
     7501 cgcgatgcgc gatcccaagt tctcccagca gttaggcgcg ttcaaggcgt ggacggcggc
     7561 tacgaatgct tcctttcgcg cggacaaggc cagggaaacc ggtcttgccg acatacagca
     7621 cgcgatcgac gggctcgagt cgacacacgg cgatgccacg ctggccgcgg aggcgcggct
     7681 gatcaaggcg cgcatgctct acttcttcag ggtcgactgg cgcgaagcgc gcgccgaggc
     7741 gcaacgcgcg gccgcggcgt tcgcacacct gcccacgccc gacgcgctca atgaggcgcg
     7801 cgcgaagtac atcgaagcgc tcgcgctcgg tgagatctcc aacgatcgcg aatcgaagga
     7861 tccgacgccc gaagaggcgc tccgactgtc caccgagatg atccagagcc tgagcgccga
     7921 cacctcggcg ctcggcccca tcgaccgtgc gcgcgcgatg ggctcgctgg cgcgcctcga
     7981 cgtgaaggcg atgaatgccg aaggcgccaa caaacacttc gggctggcgc gcgagatgtt
     8041 ccaggccgcg ggctacaccg cgggtgtgcg cgagatgcgc tggagcctgg ggctggtgtt
     8101 cgtggagctc ggcatgttcg ccgacgcggc tcgcgagttc gacgtgctgc tgcccgacat
     8161 cgacacgatc tccgatcccg agctgcatgt gcgcgcctat ctcgccgtgg gccgcgcaca
     8221 gacattctcc gatcgcaccg acgaaggcgc gcagatgctg ctgaaggcct tgcctctcgc
     8281 gcagcagtac cagcttcggc aactcgaagt gatggcactc atggggctcg gctaccacta
     8341 ccagaaccgc ggcgacatgc tgcagatggg cgtgttcttc gatgaggccc tgaaaatcgc
     8401 gcgcgaacag aaagacgtga aggaatacgt cgaagcgctc acggccgctg ccaccgcggc
     8461 gcgcgacacc ggcaatctcg atcgcgcatt cgagctcgac aaggaagcgg tgcgcaagtc
     8521 tcccacctcg gtgctcgagt gccggacgaa gatgagcctc ggcctcgact actaccgcgc
     8581 ccaggatctg ccatccgcga tcgagtggta tcgcaagtcg cttgcggtcg atctcaacga
     8641 cccgatgtcg cacatctaca ccgacgggaa gttgggactg gcgttgttcc tcatcgaaca
     8701 cgcgccgagc acggcgaagg agctcgctga agcggacaag ctgctcgccg aagcgcagga
     8761 gaccagcacg aaggtgcgcg accagtggcg cgtgatcata gcgcttcgca tccgggccgc
     8821 gctcgaagcg cgtcgcggac agaatgatgc ggcgctggcc catctcgatc aggtcttcac
     8881 gctctcgcag gaatatcgcc agcgaagcaa gagcaccgaa gcgcggtcgg gaatgctgcg
     8941 cgcggaagag cacggcttcc gtggctacct cgacatcatc ttcgcggacg tggcgaaacg
     9001 tgggcccggc gtgatccgcg cggcgacgcc gcgagagctc gcgggtctgc ggcgtctcga
     9061 gcgtgcgcgt tacgacagtt tcggcggctt gcgggtcagc gctccggacg cgaagacctc
     9121 ggcacgcgtg gaccagatct tttccgagat gggacagaag agcctgaaga tcgcgggcct
     9181 gctcaagtcg aatctcgacg ctgcacagaa agcggagttg cagggactgc agagcgacat
     9241 ggcgcgtctg cacgcggagc tcgacaacgt gcggacggtc gcggccgaca agcaggcgag
     9301 cggcgcctcg ccgccggatg cgcgccgcaa cttccgtgcg ctcgcggccg gtgccgcgca
     9361 actctcgtac gcgctcggcg cgaatcacgt ttatgccgtg gtgcgcagcg agacagggac
     9421 cgtggtgacg gtgctggcgc cttcgcgcaa ggacctcgaa gcgcagctcg gcgatttctc
     9481 gaagttcgaa gtgcagaccg cgtcgcgcga aatcgaggcg gccctcgagg agatttcatc
     9541 ggtgctgctg ccggccgggt tgttgcccga gaaatccagc acggtggaga tcgtcgccga
     9601 aggccgaatc ggcaacgttc cgttcccggc gttgcgttcg ccgacgaatg cgcagcgccg
     9661 tctcgcggaa acgcacgtgg tctccatgat catgtcgctg ttcgatcagg acgacacgct
     9721 gcgcgctcaa catgcacgtc cataccgttt cgtggcgttg gcgagcggta cgggcaccta
     9781 tcgtgcggca gtggtcgatc ccacgccgcg cttgcaggcg gcgaccaagg agatccgcgt
     9841 ggcggcggac ctcttcaccg cgcgcgacag caccgcgaag atcaaactat tcaccggcga
     9901 tgacggcacc gccacggcgc tgcgtgac
//
LOCUS       DATDEU010000465         2926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1692323, whole genome shotgun sequence.
ACCESSION   DATDEU010000465 DATDEU010000000
VERSION     DATDEU010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692323"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1378
                     /locus_tag="VIV63_11145"
     CDS             221..1378
                     /locus_tag="VIV63_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HEY6125197.1"
                     /translation="MHDEQGNRPFEIMADREGMANFDRINPKYFASLDRKMRHLSDEG
                     FVPFFETIRRDNAPSWKRYFDFNASYARFVQYLIARYGAYNMVFSGIHLDWIPKDFSL
                     TADEFNEALTWHFKKYGPLPFGQPYTTLIDRSTFKAFGAGSQAPWLTMHTVGNNPRNH
                     AIYESIEELFRLTPAMPAANLEPYYTGWNHSINRPGGETADENSERDNYFARAMMYGS
                     VLSGALAGHVHGTAAYDVTSTGEPAGWRPHIWTALRYESGAQMQHLRTFVLSEGARYQ
                     ELLLASQDLDPRSIPDAAADGLDGWSFLMRTAKQDFALAYFESKALRTRMKGFAPNAN
                     YRWTWFDPRTGSWGPALKVKTDAVGVIMSPAFESGGKQAARDVAAKIVRAP"
     gene            complement(1365..>2926)
                     /locus_tag="VIV63_11150"
     CDS             complement(1365..>2926)
                     /locus_tag="VIV63_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY6125198.1"
                     /translation="GAAADIDKRLGGVIDDWLQHRVVSGDAGHVTPIPRSLWRLRPKV
                     RTAYLLVGLGRFDRLSLDVIEHAAENLARFAEAPSYRSLATVAWSSAAGIAPADSFAA
                     QLRGLIRARAAGQTGLTRIDFHVLTRADAKAVHARLRDFVKSRPAGALRLQPLGSQRA
                     SAGTRSRRVPGVAHLIVSAESRRASHETWRSSLLTGGSSAAIYSQSKEFLSSGLDRLD
                     REFESETLTPARLKALGAKLGSLTLHPALASALLATRGQSLSVVHDAASSRVPWEALN
                     LRGWCPALDGGLSRRYATADLVPARFDADRRDQRELAVLLIANPTGDLPGAESERQRI
                     AQLLGHNKYLRLTEVVGEAATLARVTAEFESGRHDVIHYAGHASFNAQHPGDSGLMLA
                     DGELTGSHLSALARLPPLVMFNACESARLRRGPERKRRAPPRAQSIAKNLGLAETLLR
                     AGVAHYVGTHWPVDDASATAFATVFYRELMRASIGTALLKARRAVHARRSPDWADYVH
                     YGDADFRLKAP"
BASE COUNT          528 a          948 c          983 g          467 t
ORIGIN      
        1 aggcatcagc ttcgaagaag ccgtagcctg gcgcaagcgc cagggctaca actcgatctc
       61 attcatagcc gcatttccga actgggctgc ggacgaacac ggcgcgacgt tcgccaacaa
      121 ggacggcgtc taactgcgca acgcctggga aaagttcggg cactgggcgc cgaatgcaaa
      181 gatttccacg gccgatggcg ccaccaccac cgccaaggac atgcatgacg agcagggcaa
      241 ccgtccgttc gagatcatgg ccgaccggga aggaatggcg aacttcgatc gcatcaatcc
      301 aaagtatttc gccagcctcg atcgcaagat gcgccacctc tcggatgaag gtttcgtccc
      361 gttcttcgag accattcgcc gcgacaacgc gccatcctgg aagcgctatt tcgacttcaa
      421 cgcgtcctac gcgcgcttcg tgcaatacct catcgcccgc tacggcgcgt acaacatggt
      481 gttcagcgga attcacctcg actggattcc gaaggacttc agcctcacgg ccgacgagtt
      541 caacgaggcg ctgacctggc acttcaagaa gtacggcccg ctgcctttcg ggcagccata
      601 caccacgctg atcgatcgct cgacgttcaa ggcgttcgga gcaggaagtc aggcgccgtg
      661 gcttaccatg cacacggtgg gcaacaaccc gcgcaatcac gcgatttacg aatccatcga
      721 agaactgttc cggctgactc ccgcgatgcc cgcggccaac ctcgagcctt actacacggg
      781 gtggaaccac tccatcaatc gtccgggcgg agaaaccgcg gatgaaaact cggagcgcga
      841 caactatttc gcgcgagcca tgatgtacgg ctcggtgctg tcaggagcgc tcgccggaca
      901 cgtgcacggc actgccgctt acgacgtcac gagcactggc gaacccgcgg gctggcggcc
      961 gcacatctgg accgcgctgc gttatgaatc cggagcgcag atgcaacacc ttcggacgtt
     1021 cgtgctttcc gaaggtgcga gataccagga actgctgctg gcgtcacaag acctcgaccc
     1081 gcgcagcatt cccgacgcgg cggccgacgg cctcgatggg tggtcgttcc tcatgcgcac
     1141 cgcgaagcag gacttcgcgc tcgcgtactt cgagagcaag gcgctgcgta cccgcatgaa
     1201 gggctttgcg ccgaacgcga actatcgatg gacgtggttc gatccgcgga cgggtagttg
     1261 ggggccggcc ctgaaggtga agaccgacgc ggtgggtgtg atcatgtcgc cagcattcga
     1321 gtctggcggc aaacaagccg cgcgcgacgt ggccgcgaag atcgtcaggg cgccttgagc
     1381 cggaagtcgg cgtcgccgta gtgcacgtag tccgcccagt cgggcgaacg gcgcgcgtgt
     1441 accgcgcggc gtgccttgag cagcgccgtg ccgatgctgg cgcgcatgag ctcgcggtag
     1501 aacacggtgg cgaacgccgt ggcggatgcg tcgtcgacgg gccaatgcgt gccgacgtag
     1561 tgcgccaccc cggctcgcaa caacgtttcc gccaggccca gattcttcgc gatgctttgc
     1621 gcgcgcggcg gcgcgcggcg cttgcgctcg ggcccgcggc gcagtcgtgc cgattcacag
     1681 gcattgaaca tgaccagcgg cggcagccgc gcgagtgcgg acagatgaga ccccgtcagt
     1741 tcgccgtcgg ccagcatcag cccgctgtcg ccgggatgtt gggcgttgaa ggaagcatgt
     1801 ccggcgtaat ggatgacgtc gtgccggccg gactcgaact ccgccgtgac tcgcgccaac
     1861 gtcgcggctt cgcccacgac ttcggtgagc cgcaggtatt tgttgtggcc caggagctgc
     1921 gcgatgcgct ggcgttccga ttccgcaccc ggcagatcgc cggtgggatt ggcgatgagc
     1981 agtaccgcga gttcgcgttg atcgcggcgg tcggcatcga agcgcgcggg cacgaggtca
     2041 gccgtggcgt agcggcgcga caatccaccg tcgagcgccg ggcaccagcc gcgcaaattc
     2101 agcgcctccc atgggacgcg gctgctcgcc gcatcgtgga ccacggacag cgactgtccg
     2161 cgcgtggcga gtaatgcact cgcgagcgcg ggatgcagcg tcagcgatcc gagttttgcg
     2221 ccgagcgcct tgaggcgcgc cggtgtcagc gtctcgcttt cgaactcgcg gtccagccgg
     2281 tcgagccccg acgacaggaa ctccttgctc tgcgagtaga tagcggcgga gcttccgccg
     2341 gtgagaagcg aagaccgcca ggtctcgtgt gatgcgcggc gcgattcagc cgagacgatg
     2401 agatgggcga cgcccggcac gcggcgcgaa cgcgtgccgg cactggcgcg ttgcgatccg
     2461 agcggctgaa gacgcagcgc gccggcaggg cgcgatttca cgaagtcgcg caggcgcgcg
     2521 tggacggctt tcgcgtcggc gcgcgtgagg acgtggaagt cgatgcgcgt gaggccggtc
     2581 tgccccgccg cccgggcgcg tatcaggccg cgcagttgcg ctgcgaaaga atccgcgggt
     2641 gcgatgccgg ccgcggaact ccaggcaacg gtcgcgagcg aacgatagga cggcgcctcc
     2701 gcgaaacgtg ccaggttctc ggccgcgtgt tcgatgacgt ccaggctcaa gcggtcgaag
     2761 cgtcccaggc ccaccagcag gtaggcggtg cggaccttgg gccgcaggcg ccacaggctg
     2821 cgcggaatcg gcgtgacatg tccggcgtcg ccgctaacta cccggtgctg cagccagtcg
     2881 tcgatcacgc cgcccagccg cttgtcgatg tccgcggcgg cgcccg
//
LOCUS       DATDEU010000466         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_963715, whole genome shotgun sequence.
ACCESSION   DATDEU010000466 DATDEU010000000
VERSION     DATDEU010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963715"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2074)
                     /locus_tag="VIV63_11155"
     CDS             complement(<1..>2074)
                     /locus_tag="VIV63_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6125199.1"
                     /translation="LREFPDVDRPVVNIETRYRGASSDVVETRITQVIENEISGIEGV
                     ERLNSSSRDERSQINVEFSLDRDLDAAANDVRERLSRVAGRLPLEADPSQITKVDSGT
                     DPIMFLNVASTQRSVLELTDYMERYLVDQFSTLDGVANVRMNGARRYAMRVWLSRENL
                     AARRLTVNDVENSLLRENVELPAGRLESSEREFTLRTDTNLRTEEDFRNLVVGRGPDG
                     YLVRLGEVAEVRLAADDDRSLSRSNGAISLSMAVIPQSKANVLATSAAVKERLAEVQK
                     TVPKDISVEVNVDNGVFIAASLKNVVFALSETLILVLIVIFLFLGTLRATLIPAVTIP
                     VSIIAAGIVMLTLDYSINTLTLLAAVLAIGLVVDDAIVVLENIVRRIDGGEPAVLASI
                     NGSKEIGFAVIATTLVLMAVFIPVSFMTGDTGRLFGEFGVTVAAAVGFSAFVALSLTP
                     MMASKLFANGMPRSRMAGGVDRVFHNLDDRYAAGLRGAMSGRRPLWIVGGALALFSVM
                     IVVFVLGIPFGGLRITSELAPQEDRAFVRIFITAPEGSSIQFLDRQLRQVEEIAMDEV
                     RRGNALRVNVRTGGFGRAADVNTGLVIMPLELWDKRDESAAAIADRLRQRTQGISGAR
                     VNVNQAGSPLTRNSTRPLAIAMGGGDYAEIVKWRDKVMQRVAAENPRILNLESDYLER
                     QPRVIVQI"
BASE COUNT          384 a          662 c          663 g          365 t
ORIGIN      
        1 tcgatctgca cgatgacccg cggctggcgt tcgaggtagt cggactcgag attgaggatg
       61 cgtggatttt ccgcggccac tctctgcatc accttgtcgc gccatttcac gatttcggcg
      121 tagtcaccgc cgcccatggc aattgccagc ggcctcgtgc tgttgcgcgt gagcggactg
      181 cccgcctgat tcacgttgac gcgagcgccg gagatgccct gtgtgcgctg acgaagccga
      241 tccgcaatgg cggcagcgct ctcatcgcgc ttgtcccaga gttcgagcgg catgatcacc
      301 aggccggtgt tgacgtcggc ggcgcggcca aaccctccgg tgcgcacgtt gactcgcagc
      361 gcgttgccgc gtctcacctc gtccatcgcg atttcctcga cctggcgcag ttgccggtcg
      421 agaaactgga tgctggagcc ttccggcgcg gtgatgaaga tgcgcacgaa agcgcggtcc
      481 tcctgtggcg cgagttcgct cgtgatgcgc aaacccccga agggtatgcc taacacgaac
      541 accacgatca tcacgctgaa caatgccagc gcaccgccga cgatccaaag cggccgccgg
      601 cccgacatcg cgcctcgcaa gcccgccgcg tagcggtcgt cgagattgtg gaacacgcga
      661 tccacgccac ccgccatgcg cgaccggggc atgccgttgg cgaagagctt cgaggccatc
      721 atcggtgtga ggctgagcgc gacgaacgcc gagaagccga ccgccgcggc cacggtgacg
      781 ccgaactcgc cgaacagacg gcccgtgtcg ccggtcatga aggacaccgg gatgaacacc
      841 gccatgagca ccagggtggt cgcgatgacg gcgaagccga tttccttcga gccgttgatg
      901 gacgcgagca ccgcgggttc gccgccatcg atgcggcgca cgatgttctc gagcaccacg
      961 atggcgtcgt cgaccaccag gccgatggcg agcacggcgg ccaacaacgt gagcgtgttg
     1021 atggaatagt ccagcgtgag catgacgatg cccgcggcga tgatggacac cggaatggtg
     1081 acagcgggaa tcagcgtggc gcgcagcgtg ccgaggaaca gaaaaatgac aatgagcacg
     1141 aggatcagcg tttcgctgag cgcgaatacc acgttcttca gcgacgcggc gatgaacacc
     1201 ccgttgtcga cattgacctc aaccgagatg tccttgggca ccgtcttctg tacttcggcc
     1261 agacgttcct tgaccgccgc ggaagtggcg agcacgttgg ccttggattg cggtatgacg
     1321 gccatcgaca agctgatggc gccgttcgac cgcgagaggc tgcggtcgtc gtcggcggcc
     1381 aggcgcactt cggcgacttc accgagccgc accagataac catcgggccc tcggcccacg
     1441 accaggttgc ggaagtcttc ctcggtgcgc agattcgtat ccgtgcgcag cgtgaactcg
     1501 cgttcgctcg attcgagacg gccagcgggc agttccacgt tctcgcgcaa cagcgagttt
     1561 tcgacatcgt tgaccgtgag ccgacgggcc gcgagattct cacgcgacag ccacactcgc
     1621 atggcatatc gccgcgcgcc gttcatgcgc acgttggcca cgccatcgag cgtggagaac
     1681 tggtcgacga gataacgctc catgtagtcg gtgagttcga gcacgcttcg ctgcgtgctc
     1741 gccacgttca ggaacatgat ggggtccgtg ccgctatcca ccttcgtgat ctgcgaggga
     1801 tcggcttcga gcggcaaccg gcccgccacg cgtgacaggc gttcgcgcac gtcgttggcg
     1861 gcggcgtcga gatcacgatc gagcgagaac tccacattga tctgcgagcg ttcgtcgcga
     1921 ctcgaggagt tcaggcgctc aacgccctcg atgcccgaga tttcgttctc gatgacctgg
     1981 gtgatacgcg tctcgaccac gtcggaagac gcgccgcggt aacgcgtctc gatgtttacg
     2041 accggccgat cgacgtcggg aaactcgcgc aatg
//
LOCUS       DATDEU010000467         2010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1546981, whole genome shotgun sequence.
ACCESSION   DATDEU010000467 DATDEU010000000
VERSION     DATDEU010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546981"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="VIV63_11160"
     CDS             <1..1825
                     /locus_tag="VIV63_11160"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY6125200.1"
                     /translation="PSHLEAVDPVTIGRARARQDRAGVGGRARVLPLLIHGDAAFAGQ
                     GVWAETLNFADLNGFSVGGCVHVIVNNLIGFTTPPRQLHSARFAADIAKRQPVPILHV
                     NAEDIDAVIRVGRIAAEYRSTFASDVVVDLIGYRRHGHSEVDDPTISQPKLYGLIKKH
                     PPFSQIVAQQTGVDITASRRDIRTEFQAAQDEAAKASERPSLSLQPSYWQAYAGGCYN
                     PALEVDTGISADEIRDLAGTLTSYPADFRIHPKVKKLLEQRAEMGKGTRPIDFGMAET
                     LAFGSLVKTGTPIRLSGQDSRRGTFNQRHAVLVDTESEAEYVPLAHVGPNQGRCEIYN
                     STLSEVAVLGFEYGYSRDYPEALVLWEAQFGDFANGAQVIIDQFISAGEDKWGRLSGV
                     VLLLPHGYEGQGPEHSSARIERFLQLAAEDNMQICQPSTAAQYFHVLRRQVLRAWRTP
                     LIVFTPKSMLRHVSASSTVEELGRPRFLPVLPEREVENPLRVLVCTGKMGHELRQERS
                     RRGDSSNAIVVLDQLYPFPVAEVRDELLRHATAAEVVWVQEEPANMGALTYVLPRLKR
                     IIAHRRVRSIKRSASASPATGSAKAHELEQKTLIDMAFATR"
BASE COUNT          395 a          615 c          634 g          366 t
ORIGIN      
        1 cccgagccat ctggaggcag tagacccggt taccatcggc cgtgcgcgtg cgcggcagga
       61 tcgcgcgggt gtcggcggac gagcgcgtgt gctgccgctc ctcatccatg gcgacgccgc
      121 gttcgcgggg cagggagtct gggctgagac gttgaacttc gcggatctca atggattttc
      181 ggttggcggg tgtgtccacg tcatcgtcaa caacctgatc gggttcacga cccctcccag
      241 acagcttcac tcggctcggt ttgctgcgga tatcgcaaag cgccagccgg tcccgattct
      301 tcatgtcaat gccgaagaca tcgacgcagt gattcgagtg gggcggatcg ccgctgagta
      361 ccggtccacg ttcgcgagcg atgtggttgt cgatctcatt ggctaccggc gccatggcca
      421 tagcgaagtg gacgatccaa ctatcagcca gccgaagctc tacggtctta tcaagaaaca
      481 cccgccgttc tcgcaaatcg tggcccaaca gaccggcgtc gacatcaccg ccagtcgtcg
      541 cgacatcaga accgagtttc aggccgcaca agacgaagcg gcgaaggcga gcgagcggcc
      601 gtcactgtcg ctccagccaa gctactggca agcgtatgca ggcggatgct acaaccctgc
      661 cctcgaagtg gataccggca tctccgcgga cgagattcga gacctggcgg gcacactcac
      721 gagctaccct gcggactttc gcatacaccc aaaggtgaag aagcttctcg agcagcgtgc
      781 ggaaatggga aagggaacgc gccccatcga cttcggtatg gcggagacac tcgccttcgg
      841 cagcctcgtg aagaccggga cgccgattcg gctgagcggg caagacagcc ggcgtggcac
      901 gttcaaccag cgtcacgccg tgcttgtcga cacggaatcc gaagcggaat acgtcccgct
      961 cgcccacgtc gggcctaacc agggacgctg cgagatttac aactcgacgc tttcggaggt
     1021 tgcagtgctg ggtttcgagt acggctacag ccgcgactat cccgaggcgc tggtcttgtg
     1081 ggaagcgcag ttcggcgact tcgcgaacgg cgctcaggtg atcattgatc agttcatcag
     1141 tgctggtgaa gacaagtggg gccggctttc cggcgtcgtg ctgctgttgc cgcatggcta
     1201 cgagggccaa gggcccgaac actccagcgc gcgcatcgag cggttcctgc agcttgcggc
     1261 ggaggacaac atgcagatct gccagccgtc cacggccgca cagtattttc acgttctccg
     1321 acgtcaggtg ctgcgcgcat ggcgcacgcc gctcatcgtg ttcacgccga agagcatgtt
     1381 gcgacacgtg agtgcgtcgt cgacggtgga ggagttggga cgtccgcgct ttctgccagt
     1441 gctgccggag cgtgaggttg aaaaccctct gcgagtgctc gtgtgcaccg gcaaaatggg
     1501 ccacgagctc cgccaggaac gcagcagacg cggagattcc tcgaacgcga tagtcgtcct
     1561 cgaccagctg tacccgttcc cggtcgctga ggtccgtgac gagctattac gacatgccac
     1621 ggcggcagag gtggtgtggg ttcaggaaga gccggcgaac atgggagcgc taacgtatgt
     1681 gctgccgcgc ttgaagcgaa tcatcgcaca tcgccgcgtg cgatcgatca agcgttctgc
     1741 gtctgcaagc ccggcaaccg gatcggccaa ggctcacgag ctggaacaaa agacgctcat
     1801 tgacatggcg ttcgctacgc gctgacggcg ccccattcaa tccgctggct gcagtgagcg
     1861 caagtcaaca ctttcgtcac gcagccgatc gagatccacg gtgcgccgcg acttgatgag
     1921 cggcatgaca cgccgcacgt ctttccctcg gttgagaccg acagcggcat cgattcggcc
     1981 gtcgttcaca tagcacgcca gaaagttgcg
//
LOCUS       DATDEU010000468         6693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1838669, whole genome shotgun sequence.
ACCESSION   DATDEU010000468 DATDEU010000000
VERSION     DATDEU010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838669"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VIV63_11165"
     CDS             complement(<1..587)
                     /locus_tag="VIV63_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY6125201.1"
                     /translation="MAPISDVVRKHLESYLKHLGTERRLSPHTSSNYARDLRALADYM
                     DRGNIPDLSKVDSQHVRVFAARAHAGGLSPRSVQRRLSAVRGFFNYLVRERIVPSNPA
                     VDIRAPKAARRLPGTLDVDQLNQLLDIPPEDVLAVRDKAIMELFYSSGLRLDELVGLD
                     LGHLDLADRTVRVLGKGRKTRVVPVGRKAVDALRI"
     gene            complement(626..1333)
                     /locus_tag="VIV63_11170"
     CDS             complement(626..1333)
                     /locus_tag="VIV63_11170"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY6125202.1"
                     /translation="MSTQTARGVTAGQTDEERIERYLSLNPDFFERHQPLLSRMRLPH
                     MRTGSTVSLVERQVEVLREQKTDADRRLAEFIAVARANDTLAERIHRFTRRMLRAPNP
                     AVAITTLEASLREDFDAFHSVLLLTTPIASLAKVEVEPFLRKLPADDANIRTFEALLA
                     TGKPRCGQVRDTQRDFLFGPEGTSIGSVALVPLGENGSVGLLALGSAERERFHPGMST
                     EFLKRMGELITDALARP"
     gene            complement(1349..2185)
                     /gene="dapF"
                     /locus_tag="VIV63_11175"
     CDS             complement(1349..2185)
                     /gene="dapF"
                     /locus_tag="VIV63_11175"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY6125203.1"
                     /translation="MQLEFTKMHGLGNDFIVFDAPRDGWLPNAEQWRTLSARHTGIGF
                     DQALVLEPARRAGTDVYYRIFNADGGEVEQCGNGVRCLASFLHRRGSVANGKGEIVLD
                     SPAGPIHARVHDANLISVDMGVPNFDPKSLPFEASAEAHVYPLNVAGTEVEIGAVSMG
                     NPHAVLTVNSVASAPVDRLGPAIERHPRFPKRVNVGFMEIVDRAHIRLRVFERGVGET
                     LACGTGACAAVAVGRRHGRLDSTVEVELPGGKLEITFAAPGEHIWMKGPTAVSFTGRV
                     EI"
     gene            2216..2809
                     /locus_tag="VIV63_11180"
     CDS             2216..2809
                     /locus_tag="VIV63_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125204.1"
                     /translation="MRLAPVALAALAIFGIYKLYSGREIAHAPGVLVAEDPRQRVIES
                     ADVIDLGEFHLRPRADFSATVRILRREDYSLGALAKLVPTDFAVGWGPMSDSAVLSNI
                     EISQGNRFYYWRTDHWPIDRADIERHSANWHVIAANPGIGEVLGRLRAGTVVELRGRL
                     VDIEGGESGMRTSLSRNDTGAGACEILLVESATIAGS"
     gene            2825..3370
                     /locus_tag="VIV63_11185"
     CDS             2825..3370
                     /locus_tag="VIV63_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6125205.1"
                     /translation="MNADVVSQKAAVQIRRATVADAEALTEIGARTFFETFAADNTPE
                     DMQMFLSSTWQADLQRAEILDAGMDTLLACDASGAIAGFAQVRVAHPPAGIAARAPVE
                     LKRFYVDKPWHGQGLARVLMDSAEQAARARGGKEFWLGVFERNERARAFYRKCGFSRV
                     GAQIFVVGTDPQTDDVMLKVL"
     gene            3370..3759
                     /locus_tag="VIV63_11190"
     CDS             3370..3759
                     /locus_tag="VIV63_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6125206.1"
                     /translation="MTARLEHVGLWVSDIDTVAAFYAKYFGAQPGELYRNPRKGFESR
                     FLTFDGGARLEVMTRVDVTGRAPAEQLGLAHVALCIGDEAAVDALAARFRADGIEPDS
                     GPRRTGDGYYECVVRDPEGNRVEIAAG"
     gene            3783..6347
                     /locus_tag="VIV63_11195"
     CDS             3783..6347
                     /locus_tag="VIV63_11195"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY6125207.1"
                     /translation="MKSVVLLVSALLFASAGAAQEKPSAWPQITGSAKPWTRWWWPAS
                     AVDPANLTRQLEAIAAAGIGGVEITPIYGARGSEARYVDFLSPKWMEMLEHTTREAKR
                     LGLGVDMATGTGWPFGGPTVGAADGSSILALIDGKLTGKPTAMKVKRPAPGGEGLVLD
                     PYSPAALDRYLRPFSKAFAGFPRDALRAQFHDSFEYYQASWTAELPKVFQQMHGYDIQ
                     AYAAQITGEQPLDDDTLGRIKGDLRRTIAKLHLDYVNAWVTWAHEEGFKARNQAHGAP
                     GNLLDLYAVADIPETESFGLTQLPIAGLRGDAVGVSIDPDPPEVTIGRFASSAAHVAG
                     RPLVSSETLTWLRENFRESPAAAKPQLDRLFAAGINHIFYHGTVYSPADAEWPGWFFY
                     ASTQLTPSNPLWDDFGAMHAYVARVQSVLQAGAPDNDILLYWPFDDVVEHSDGLMEHY
                     GVHENKWLTESAAGRLSAKLVHAGFGFDFISDAQLLRLKVDGRTFIAPGGGRYRTLVI
                     PATRRMTVETLTQLRELRKRTERVIIESLPQDVPGHGRLEERRSTLRGLLTSPALRLA
                     VAGDHIVPKLLEHGVRQEQAPQAGLAHIRRARTDGHDYFFANLGAKPFDGWLQLGVSA
                     ESALLLDPLDGKAGAAAVKLGADGRLQVYLQLASGQSALVRTYRTKRAPSLQAWRYAK
                     AAGDGIAPQGEWQVEFSKGGPVLPGPARMSTLASWTSLNDAEARRFAGTARYRVEFDA
                     PAAADAWELDLGDVRETARVTINGQRLATVWSLPFKVRLDALKPRGNVLEVEVTNLPA
                     NRIRDLDVRKVPWKVMRDINLVSVKYRPFDASAWEVEPAGLLGPVTLVPLQLVSPR"
     gene            complement(6334..>6693)
                     /locus_tag="VIV63_11200"
     CDS             complement(6334..>6693)
                     /locus_tag="VIV63_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY6125208.1"
                     /translation="GTWAVVGLSGNPSRTAYAIAGLLQRRGKRIVPVHPSAPVVLGEQ
                     GYATLADIPFPVDVVDVFRRPEHVAAIVEDCIRLKAPALWLQDGVVDDAAAQKARDAG
                     LFTVMNRCMFRDRSTLG"
BASE COUNT         1120 a         2141 c         2318 g         1114 t
ORIGIN      
        1 cagatgcgca gcgcgtccac agccttgcgg cccacaggaa cgacgcgcgt cttacgaccc
       61 ttgccgagca cgcgtaccgt gcgatccgcc agatcgagat ggcccaagtc gaggcccacg
      121 agctcgtcga gccgcagtcc ggaggaataa aacagctcca tgatggcctt gtcgcgcacc
      181 gccagtacgt cctcgggcgg aatgtccagg agctggttga gctggtcgac gtcgagcgtg
      241 ccgggcaggc gccgcgccgc cttgggcgcg cggatatcga cggcggggtt gctgggcacg
      301 atgcgctcgc gaaccaggta gttgaagaag ccacgcaccg ccgacagccg gcgctgcacg
      361 ctgcgcgggc tcaggccccc ggcatgcgct cgcgccgcga agacgcgcac gtgctggctg
      421 tcgaccttgc tcaagtccgg gatattgccg cggtccatgt agtccgcgag cgcgcgcaga
      481 tcgcgcgcgt agttagagga ggtgtgcggc gagaggcgcc gttcggtgcc gagatgcttg
      541 aggtaacttt ccaggtgctt gcgcaccacg tcggaaatcg gcgccacttc tttcaaagaa
      601 gggcggcgcc gtccgtcagc ggggttcacg gccgtgcgag agcatccgta atcagttcgc
      661 ccatgcgctt caggaattcc gtgctcatgc ccggatggaa acgctcgcgc tcggcgctgc
      721 cgagagccag gaggcccacc gaaccgttct cgcccagcgg caccagcgcc acggagccga
      781 tgctcgtgcc ttcggggccg aacaggaaat cgcgctgtgt atcgcgcacc tggccgcaac
      841 gcggcttgcc cgtggcgagc agtgcttcga acgtgcggat gttggcgtca tcggccggca
      901 gcttgcgcag gaacggttcg acttcgacct tggcgagcga ggcgatgggc gtggtgagca
      961 gcagcacgga atggaaagca tcgaagtctt cgcgcaggga ggcttcgaga gtggtgatgg
     1021 ccacggcggg attcggcgcg cgcagcatgc ggcgcgtgaa gcggtggatg cgttccgcga
     1081 gggtgtcgtt ggcgcgcgcg acggcgatga attctgcgag gcggcgatcc gcgtcggttt
     1141 tctgttcgcg cagcacttcc acctgtcgct cgaccagcga gacggtggag ccggtgcgca
     1201 tgtgcggcag gcgcatgcgc gagagcagcg gctgatgacg ttcgaagaag tcggggttga
     1261 ggctgaggta acgctcgatg cgttcttcat ccgtttgtcc cgcggtcact ccacgtgcgg
     1321 tctgcgtgct catgctttat tcatctccct agatttccac ccggccggtg aaagatacag
     1381 cggtcgggcc tttcatccaa atatgttcgc cgggcgcggc aaaggtgatc tcgagctttc
     1441 cacccggaag ttcgacttcg acagtcgaat cgagccggcc atggcgtctg cctaccgcga
     1501 cagcggcgca ggcgccggtg ccgcaggcca gtgtttcgcc gacgccacgt tcgaatacgc
     1561 gcaaccggat gtgcgcgcgg tcgacgatct ccatgaaacc gacgttcacg cgcttcggaa
     1621 agcgcgggtg acgttcgatg gcggggccaa gacggtccac aggggcgctc gcgaccgagt
     1681 tgaccgtgag cacggcgtgc ggattgccca tcgacacggc gccgatctcg acttcagtac
     1741 ccgccacgtt gagcggatag acgtgcgcct ctgccgaggc ttcgaacggc agcgacttcg
     1801 ggtcgaaatt gggcacgccc atgtcgaccg agatgaggtt ggcatcatga acccgcgcgt
     1861 ggatgggccc ggcggggctg tcgagaacga tttcgccctt gccgttggcc acgcttccgc
     1921 ggcggtgaag gaaggaggcc aggcaacgca cgccattgcc gcactgctcc acctcgccgc
     1981 catcggcgtt gaagatgcgg tagtagacgt ccgtgccggc gcggcgcgcc ggttcgagca
     2041 ccagcgcctg gtcgaaacca atcccggtat gacgcgcgga caacgtgcgc cactgctcgg
     2101 cgttcggcag ccagccgtcg cgcggtgcgt cgaacacgat gaaatcgttg ccgaggccgt
     2161 gcatcttggt gaactcgagc tgcatggccc gaagaatacc ttacgatccg gcctcatgcg
     2221 tctcgctccc gtcgcgctgg ccgcgctcgc gatcttcggt atctacaagc tctactcggg
     2281 gcgcgagatc gcgcacgcgc ccggagtgct ggtcgccgaa gatccgcggc agcgagtcat
     2341 cgagtccgcg gacgtcatcg acctcggcga gttccacctg cggccgcgtg ccgacttctc
     2401 ggccaccgtg cgcatcctgc gccgcgaaga ctattcgctt ggcgcgctgg cgaagctcgt
     2461 accgaccgac ttcgccgtgg gctgggggcc gatgtccgat agcgccgtgc tctcgaacat
     2521 cgagatcagc caggggaacc gcttttacta ttggcgcacg gatcactggc ccatcgatcg
     2581 cgccgacatc gagaggcact cggccaactg gcacgtgatc gcggcgaatc ccggcatcgg
     2641 tgaggtgctc gggcggctgc gggccggcac cgtggtggaa ttgcgagggc ggctcgtgga
     2701 catcgaaggc ggcgagagcg gcatgcgcac ttcgctgtct cgcaacgaca cgggagccgg
     2761 cgcctgcgag atactgctgg tggagtccgc cacgatcgcg gggtcctgaa cgagaggggc
     2821 gacgatgaat gccgatgtag ttagtcagaa ggctgccgtg cagatccggc gggccacggt
     2881 ggccgatgcg gaagcgctca cggagatagg cgcccgcacc ttttttgaaa ccttcgccgc
     2941 cgacaacaca cccgaagaca tgcagatgtt cctgtcttcg acctggcaag cggacctgca
     3001 gcgcgccgaa atcctggatg ccggcatgga tacgctgctg gcctgcgatg cctcgggtgc
     3061 gatcgcggga ttcgcacagg tgcgcgtggc tcatccgccc gcgggaatcg ccgcgcgcgc
     3121 tcccgtggag ctcaaacgtt tctacgtcga caagccctgg cacgggcaag gcctggcgcg
     3181 cgtgctcatg gattccgctg agcaggcggc gcgggcacgc ggcgggaagg agttctggct
     3241 gggtgtcttc gagcggaacg agcgcgcgcg ggcgttttat cgcaagtgcg gtttcagcag
     3301 ggtcggcgcg cagatcttcg tggtgggcac ggatccgcag acggacgacg tgatgctcaa
     3361 ggtgttgtga tgaccgcgcg cctcgagcac gtgggcctgt gggtcagcga catcgacacg
     3421 gtcgcggcgt tctacgcgaa gtatttcggc gcgcaaccgg gcgagctcta tcgcaatcca
     3481 cgcaagggtt tcgaatcgcg gttcctcacg tttgacggtg gtgcgcggct cgaagtcatg
     3541 acgcgcgtgg atgtcaccgg acgcgcgccc gcagagcaac tcggtctcgc gcacgtggcg
     3601 ctttgcatcg gtgacgaagc ggccgtggat gcgttggcgg cgcggttccg tgctgacgga
     3661 atcgagccgg acagcgggcc gcgccgcacc ggagacggat actacgagtg cgtggtgcgt
     3721 gatccggaag gcaatcgcgt cgagatcgcc gcgggataaa tgcttcacca gggacgggga
     3781 ccatgaaatc agtcgtactg ctcgtatccg cgctgctgtt cgcatcggcg ggtgctgcgc
     3841 aggaaaaacc ttccgcctgg ccgcagatca ccggcagtgc caaaccgtgg acgcgctggt
     3901 ggtggccggc cagcgccgtg gatcccgcga acctcacgcg tcagctcgaa gcgatcgccg
     3961 cggcgggtat cggtggtgtc gagatcacgc ccatctacgg cgcgcgcggt agcgaggcgc
     4021 gctacgtgga tttcctctcg ccaaagtgga tggagatgct cgagcacacg acgcgcgagg
     4081 cgaagcggct gggattgggc gtggacatgg cgaccggcac gggctggccg tttggcggcc
     4141 cgacggtggg agcggccgac ggttcgagca tcctcgcgct gatcgacggc aagctgaccg
     4201 gcaagccgac ggcgatgaag gtgaagcgac cggccccggg cggcgaaggc ctggtgctcg
     4261 atccttattc gccggccgcg ctcgatcgct acctgcggcc gttctccaag gcgttcgcgg
     4321 gttttccccg cgacgccctg cgagcccagt tccacgactc tttcgagtat taccaggcga
     4381 gctggacggc cgagttgccg aaggtcttcc agcagatgca cggctacgac atccaggcgt
     4441 atgccgcgca gatcacgggc gaacagcctc tcgacgacga cacgctcggc cgcatcaagg
     4501 gcgacttgcg ccgcacgatc gcgaagctgc atctcgacta tgtgaacgcg tgggtcacgt
     4561 gggcccacga agaaggtttc aaggcgcgca accaggcgca cggcgcaccc ggcaatctgc
     4621 tcgacctcta cgcggtggcc gatattcccg agaccgaatc cttcggcctg acccaactgc
     4681 cgatcgccgg tctgcgtggc gacgcagtcg gagtcagcat cgacccggat ccacccgaag
     4741 tgacgatcgg ccgctttgcc tcgtccgcgg cgcacgtcgc gggccgaccg ctggtgtcga
     4801 gcgagacgct gacctggctg cgcgagaact tccgcgagtc gcccgccgcg gccaaaccgc
     4861 agctcgaccg gctcttcgcc gcgggcatca accatatttt ctatcacggc accgtgtact
     4921 cgccggccga cgcggaatgg ccgggctggt ttttctacgc atcgacgcag ctcacgccct
     4981 ccaacccgct gtgggacgac ttcggcgcga tgcatgcgta tgtcgcgcgt gtgcagtcgg
     5041 tcctgcaggc gggcgcgccc gacaacgaca tcctgttgta ctggcccttc gacgacgtgg
     5101 tcgagcacag cgacggactc atggagcact acggcgttca tgagaacaag tggctgaccg
     5161 aatccgccgc cggccgcctg tcggcgaagc tggtgcacgc aggcttcggg ttcgatttca
     5221 tttccgacgc gcagctcctg cggctgaaag tggacggcag gactttcatc gcgcccggtg
     5281 gaggccgcta ccggacgctc gtgattcccg cgactcgccg catgacggtg gaaacactga
     5341 cgcagcttcg cgaactcagg aagcgaaccg aaagggtcat catcgaatcc ctgccgcagg
     5401 acgttccggg tcacgggcgg ctggaagagc gccgctcgac gttgcgtggg cttctcacct
     5461 cgccggccct gaggctggcc gtagcgggcg atcacatcgt gccgaagctc ctggaacacg
     5521 gcgtgcgcca ggagcaggcg ccgcaggcgg gactcgcgca catccgccgc gcgcgcaccg
     5581 atggtcacga ctacttcttc gcgaatctcg gcgcgaagcc gttcgacggc tggctgcagt
     5641 taggcgtctc cgccgaatcg gccctgttac tcgacccgct cgacggcaag gccggcgccg
     5701 cggccgtgaa gctcggcgcg gatggccggc tccaggtcta tctgcagctc gcatcgggcc
     5761 agtcggcgct ggtgcgtacc taccggacga agcgcgcgcc ttccttgcag gcctggcgtt
     5821 atgcgaaagc ggcgggcgat ggtattgcgc cgcagggcga atggcaggtc gagttctcga
     5881 agggtggtcc ggtgctgcca ggtccggcgc gcatgagcac gcttgcgagc tggacctcac
     5941 tcaacgacgc agaggccagg cgcttcgcgg gcactgcgcg ttaccgcgtt gaattcgacg
     6001 cgcccgcggc ggcggacgcg tgggaactcg acctgggcga cgtacgtgaa accgcgcgcg
     6061 tgacgatcaa cggccagcgg ctcgcgacgg tgtggagcct gccgttcaag gtgcgtctcg
     6121 atgcgctgaa gcctcgcggc aatgtgctcg aggtcgaagt cacgaacctg ccggcgaacc
     6181 ggatccgcga tctcgacgtg cgcaaggtgc cgtggaaggt gatgcgcgac atcaacctcg
     6241 tcagcgtgaa gtaccgtccg ttcgacgctt ccgcgtggga ggtcgagccg gccggactgc
     6301 tcgggcccgt gacgctggtg cctctgcagc tagttagccc aaggtagacc ggtcgcggaa
     6361 catgcagcgg ttcatgaccg tgaagaggcc ggcgtcgcgg gccttctgcg cggcggcatc
     6421 gtcgacgaca ccatcctgca gccacaatgc cggcgccttc aggcggatgc agtcctcgac
     6481 gatggccgcg acgtgttccg ggcggcggaa gacgtcgacc acgtcgacgg ggaaggggat
     6541 gtcggcgagg gtcgcgtagc cctgctcgcc gaggaccacc ggcgccgacg ggtggaccgg
     6601 cacgatccgc ttcccgcgcc gctggagcag ccccgcgatg gcgtacgccg tccgcgaggg
     6661 gttgccggac aacccgacga ccgcccacgt ccc
//
LOCUS       DATDEU010000469         2494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1692918, whole genome shotgun sequence.
ACCESSION   DATDEU010000469 DATDEU010000000
VERSION     DATDEU010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692918"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..465)
                     /locus_tag="VIV63_11205"
     CDS             complement(25..465)
                     /locus_tag="VIV63_11205"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEY6125209.1"
                     /translation="MALRILDLAARLLLALGVGCFLLAGYLAWRTLSFSGGAIPVTGE
                     VVSYREIPDGDSTRYVPRIRFVTADGDIVTVGGQLAGTSKRFEIGAKVPMVYDPREPM
                     HARVALFVDNWLGPCIAVVVGLVGLAGGLLVRRSVRRELAKSRA"
     gene            522..668
                     /locus_tag="VIV63_11210"
     CDS             522..668
                     /locus_tag="VIV63_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125210.1"
                     /translation="MIEPEKKGSKWPWILVPVAAFSIFFGLRECQHRIPPAEHAAPAS
                     PTPQ"
     gene            complement(679..1257)
                     /locus_tag="VIV63_11215"
     CDS             complement(679..1257)
                     /locus_tag="VIV63_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6125211.1"
                     /translation="MSLVAAIHVAVLFAIARSLGIGAPIEVPPPIEGEFIEEKRPPVD
                     VPPLPNPDLTSSYQNQTLPPPDLARVDFDQDVITPPPDIDIEGPPVVEPPRVELVGVR
                     QDPRRPLSKPQYSARMIREGNQGTIEIEVFVQPNGRVADARVAKSTGFDELDLAAIAE
                     AKRNWRLIPATRDGVPIAQWHRLRVTFKLNEQ"
     gene            1710..>2494
                     /locus_tag="VIV63_11220"
     CDS             1710..>2494
                     /locus_tag="VIV63_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6125212.1"
                     /translation="MSRRQPKVGFVSLGCPKALVDSERIITQLRAEGYQVAPSYDSAD
                     VVVVNTCGFIDSAVDESLDAIGEALEQNGKVIVTGCLGARPEKILDRHPRVLKVTGPQ
                     AYEDVMNAVHEHLPPKHDPFVDLVPPQGIRLTPRHYAYLKISEGCNNKCSFCIIPSMR
                     GLLASRPIDDVLREGEKLARAGVRELLVISQDTSAYGVDIRYREGEWRGQKYETRFLD
                     LARGLGELGIWVRMHYVYPYPHVDDVIPLMAERKVLPYLDIPF"
BASE COUNT          453 a          810 c          805 g          426 t
ORIGIN      
        1 agtaggcctt gtcgaactgc ccgctcatgc gcgggacttg gccagctcgc ggcgcaccga
       61 gcggcgcacc agcaatccgc cggcgagccc gaccagcccg accaccaccg cgatgcaggg
      121 gcctaaccag ttgtcgacga acagcgccac gcgcgcgtgc atgggttcgc gcgggtcgta
      181 gaccatgggc actttcgcgc cgatctcgaa acgcttcgac gtgcctgcga gttgtccgcc
      241 gacggtcacg atatcgccat ccgccgtcac gaagcggatg cgcggcacgt agcgcgtgga
      301 gtcgccgtcg gggatttcgc ggtagctaac cacctcgccg gtgacgggga tcgcaccgcc
      361 cgagaaactc agtgttcgcc aggcgagata ccccgccagg aggaagcaac ctacgccgag
      421 agccagcagc aaccgtgcgg caaggtccag aattcgaagc gccatttgca ggattcacgg
      481 aatcacaata gattcgcgag tctattcgat tggggagcac gatgatcgag ccggagaaga
      541 agggatccaa gtggccctgg attttggtac ccgttgccgc gttcagcatt ttcttcggcc
      601 tgcgcgaatg ccagcaccgg attccgcctg cggaacacgc ggcgccggcc agcccgacgc
      661 cgcaataagc cccgccaatt actgttcgtt cagcttgaaa gtcacgcgca ggcggtgcca
      721 ctgcgcgatc ggcacgccgt cgcgcgtcgc cggtatcagt cgccagttgc gcttcgcctc
      781 ggcgatcgcc gccagatcca gctcatcgaa tcccgtgctc ttggccacgc gcgcgtcggc
      841 cacgcggccg ttcggctgca cgaagacctc gatttcaatc gttccctggt tgccctcgcg
      901 gatcatgcgc gccgagtact gcggcttcga cagcgggcgg cgcgggtcct ggcggacgcc
      961 gacgagttcg acgcgcggcg gctcaactac cgggggccct tcgatgtcga tgtccggcgg
     1021 tggcgtgatg acgtcctgat cgaaatcgac tcgcgcaaga tccggtggcg gcagcgtctg
     1081 attttggtac gacgatgtga ggtccgggtt gggcaggggc ggaacatcca ccggtggccg
     1141 cttttcttcg atgaactcac cttcaatggg gggtggcact tcgatcggtg cacctatgcc
     1201 caggcttcgc gcgatggcga agagcacggc gacgtgaatc gccgcgacca gactcattcg
     1261 actcaatgcg cccaacgggc ggctctgcgc agcaacagca gccatgacgg acctccgcat
     1321 gcacccccgg cgagggtgtc ttgagaggtg aacgtggcgg gaaaggacgg cgggttagga
     1381 gacaggtgcg agttccctgc aactaacaag ttgcccgcgt gccaactcgt gtcacccgat
     1441 cgatcgcccg gcgctctcga acccgacata tagcggcggc ggcaggacct ccatgtccct
     1501 catccgcgag gtcatgggcc cgacgccggg gacgagccgc aacctcggca ttacggccgg
     1561 tctggcggaa cagtcgctgg ccaatgggcc gcccaacccg cccgaaaaca ctcgtaagag
     1621 cccgaattaa gggcattttt cgccccggag ggcatatgtc agcctcgcgt cgcttgaggg
     1681 tccgcgcgcg tgtaccattg cgccccctca tgagccgccg ccaaccaaaa gtcgggtttg
     1741 tcagcctcgg gtgccccaaa gcgctcgtgg actccgaacg catcatcacc cagctgcgcg
     1801 ccgagggtta ccaggtggcg ccgagttacg actcggctga cgtggtcgtc gttaacacct
     1861 gtggcttcat cgactccgcg gtggacgaat cgctcgatgc gatcggcgag gcgctcgagc
     1921 agaacggcaa ggtgatcgtc accggttgtc tcggtgcccg gccggagaag attctcgacc
     1981 ggcatccgcg cgtgctgaag gtcaccgggc cgcaggccta tgaagacgtg atgaacgcgg
     2041 tgcacgagca cctgccgccg aagcacgatc cgttcgtgga cctggtgccg ccgcagggca
     2101 tccgcctcac gccgcggcac tacgcgtatc tcaagatctc cgaaggctgc aacaacaagt
     2161 gcagcttctg catcatcccg tcgatgcgcg ggttgttagc cagccggccc atcgacgatg
     2221 tgctgcgcga gggcgagaag ctggcgcgcg cgggtgttcg cgagctgctg gtgatttcgc
     2281 aggacacgtc ggcctacggc gtggacatcc gctaccgcga aggcgagtgg cgcgggcaga
     2341 aatacgaaac acgattcctg gacctggcgc gcgggctcgg cgagctcgga atctgggtgc
     2401 gcatgcacta cgtgtatccg tatccgcacg tggacgacgt gatcccgctg atggcggagc
     2461 gcaaggttct gccctatctc gacatccctt tcca
//
LOCUS       DATDEU010000470         4514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1401981, whole genome shotgun sequence.
ACCESSION   DATDEU010000470 DATDEU010000000
VERSION     DATDEU010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401981"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VIV63_11225"
     CDS             complement(<1..766)
                     /locus_tag="VIV63_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6125213.1"
                     /translation="MTLKVLHLRDSQWIDGPGRTILETGAHIDPKRVEFHIAAFVANE
                     GVVHPLIAGARQRGIRAHSIVDGGGIDQRAVNRIVELVREHSIDVIHTSELRSNIYGW
                     LARRRCKVKLVRTAHGWIANDFKRRLMRTADKLLLSRADAVIAVSDATRDLLPWWMRG
                     RALVLRNALMFDRYGADIVNAPRPSMATGMPIVVANVGRLSPEKGQALLIEAIAQIAP
                     HHPTLQLLIAGVGPLEAELRQLANDRGIGERVRFLGF"
     gene            complement(763..1893)
                     /locus_tag="VIV63_11230"
     CDS             complement(763..1893)
                     /locus_tag="VIV63_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6125214.1"
                     /translation="MSLVFWISVLAIAYPYAIYPAVLVFMNRLAGRRAHVGEPSARPS
                     VTLILPVHNEAKRIEAKMRNILELDYPAEKMQVLVIGDACTDDSLARAQAAGGPGVET
                     FNVTPRGGKASALNAALERARGEILVFTDAAIMLERESLARLVAHFHDPAVGAVSGED
                     YVEGGGSEGLYGRIELLLRREEALAHSIAGASGCFYAQRRSLTPPFISGMAPDFLSVL
                     NVVRAGSRALAEPAARGAMTATKSQSAEYTRKARTFLRGITALFGNVALMNPFRHPTF
                     AFILVSHKLMRWLGPLFMIAALAASFALRAEPFYAFAFYAQVAAYGAATVGLLVPAVA
                     ERVGLVRLAAFFVLVNLAALQALLRWLSGERLEVWEPTRRPQ"
     gene            complement(1904..2896)
                     /gene="nadE"
                     /locus_tag="VIV63_11235"
     CDS             complement(1904..2896)
                     /gene="nadE"
                     /locus_tag="VIV63_11235"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEY6125215.1"
                     /translation="MGKQQLDARVLELDYDKEIERIGARLRELLSKEVMRRGFVCAMS
                     GGIDSSVSSALCVKALGPGKVYGIMLPERDSSGFSTARGKQLAEHLKIRHEVFDIAPA
                     LEGIGCYRQRDEAIRRVFPEYTDGWKNKIIIKGGLEGRINHFELVVQTPAGELKTARL
                     PLPEYLQIVAATNHKQRIRKTVEYYHADRLNYAVIGTPNRLEYDQGFFVKNGDGSADV
                     KPIAHLYKSQVYALARHMKLPAEICAAIPTTDTYSLAQGQDEFYFALPYAQMDLALYA
                     LNHGYSAAELGEALKLSEKQAQQVFNDILAKRKATRYMHLKPQLVEPVKELNLP"
     gene            complement(2908..4407)
                     /locus_tag="VIV63_11240"
     CDS             complement(2908..4407)
                     /locus_tag="VIV63_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY6125216.1"
                     /translation="MTTLVAAIEDAARRNAGAPALSWHKATWSYADLAGAIARVSDAL
                     PVNTGDRVALLLRNSPQFAAAFYGVLRAGAAAVPLNVQERAGVLARQIEHCGARVVLA
                     DAAHVEFAALRDALSGWDVTLLPLELVDAPNGCEAFCASLGAHPPREALTITPDTLAC
                     IIYTSGTTGRPKGVMLSHGNLSSNARIIANYLEIVPTDKVMCPLPFHFSYGMSVLNSN
                     LSAGAHLVLEDNLAFPQVLVKRLQDEAITAFAGVPATYALLLGRTQLADFDLSRLRYV
                     TQAGGAMPRPQIQALRDALPHVRLFVMYGQTEATARLTFLPPDELDEKLGSVGLPLPG
                     VDIEIRDPSGRVVPDDEVGELCARGPNVMMGYWDDAALTVQAVRNGWLHTGDLAHRDG
                     DGYLYIDGREVEMIKVGAFRVSPQEIEEVLATLPGVQEVAVTGMPDALLGQAIKAVIV
                     LREGAAPDVMGVKAHCRAHLAQYKIPKVVEFAVELPRTSSGKVQRYKLA"
BASE COUNT          783 a         1490 c         1471 g          770 t
ORIGIN      
        1 cgaagcccaa gaaacgcaca cgctcgccga tgccgcgatc gttggcgagc tgccgaagtt
       61 cggcttcgag cggccctact ccggcaatca gcagttgcag cgtcggatga tgcggcgcga
      121 tttgcgcgat cgcttcgatg agcagggcct ggcccttctc gggcgaaagc cggcctacgt
      181 tcgcgacgac aatcggcatg ccggtcgcca tcgacgggcg cggcgcgttg acgatgtcgg
      241 cgccgtaacg atcgaacatg agcgcgttgc gcaacaccag tgcgcggccc cgcatccacc
      301 agggcagcag gtcgcgcgtc gcatcggaga ccgcgatcac cgcatcggcg cggctcaaca
      361 gcagtttgtc ggccgtgcgc atgaggcgcc gcttgaaatc gttggcgatc cagccgtgcg
      421 cggtgcgcac cagcttcacc ttgcagcgcc gccgcgccag ccatccgtag atgttggaac
      481 gcaattcgct ggtgtggatc acgtcgatgg aatgttcacg caccagctcg acgatgcggt
      541 tcaccgcgcg ctgatcgatt ccgccgccat cgacgatgga gtgcgcgcgg ataccgcgct
      601 gacgcgcgcc cgcgatcagc ggatgcacca cgccttcgtt cgccacgaag gcagcgatat
      661 ggaactcgac gcgcttcggg tcgatatgcg caccggtctc gaggatggtg cggccgggcc
      721 cgtctatcca ctgcgagtcg cgaagatgca gaaccttgag cgtcattgcg gacgccgggt
      781 gggttcccag acctcgaggc gctcgccact gagccagcgc agcagcgcct ggagtgcggc
      841 gagattcacc agcacgaaga acgcggcgag ccgcacgagg cccacgcgct cggcgacggc
      901 cggcaccagc aagcccaccg tcgcggcacc atacgcggcg acctgcgcgt agaacgcgaa
      961 tgcgtaaaag ggctcggcac gcagggcgaa cgaagccgcg agcgccgcga tcatgaacag
     1021 cggcccgagc cagcgcatga gcttgtgcga aacgaggatg aacgcgaacg tcggatgccg
     1081 gaacggattc atcagggcga cgttgccgaa cagcgcggtg atgccgcgca ggaaggtgcg
     1141 cgccttgcgc gtgtattcgg cgctctggct cttcgtggcc gtcatggcgc cgcgcgcggc
     1201 gggttccgcg agtgcgcgcg agcccgcccg caccacgttg agtaccgaca ggaaatccgg
     1261 cgccatgccg gaaatgaacg gcggcgtgag agacctgcgc tgcgcataga aacagccgct
     1321 ggcgccggcg atggaatgcg ccagcgcctc ctcgcggcgc agcagtagtt cgatgcggcc
     1381 gtagaggcct tcgctgccac caccctcgac gtaatcttca cccgaaaccg cgcccaccgc
     1441 cgggtcgtgg aaatgcgcga ccagccgcgc gagtgattcg cgctcgagca tgatggcggc
     1501 gtcggtgaac accaggattt cgccgcgagc acgctcgagc gccgcgttca gcgccgaggc
     1561 cttgccgccg cgtggcgtca cgttgaaagt ctcgacgccc ggaccacccg cggcctgcgc
     1621 acgcgccagc gagtcgtccg tgcacgcgtc gccgatgacg agcacctgca tcttctccgc
     1681 gggatagtcg agttcgagga tgttgcgcat cttcgcctcg atgcgcttcg cctcgttgtg
     1741 caccggcagg atcagcgtga ccgacgggcg tgccgaaggc tcacccacgt gcgcacgccg
     1801 cccggccaga cggttcatga acaccagcac agccgggtag atcgcgtacg ggtacgcgat
     1861 cgccagtacg ctgatccaga acaccaggga catgcggcgg cctttacggc aggttgagct
     1921 ctttcacggg ctcgacgagc tgcggcttga ggtgcatgta acgcgtggcc ttgcgcttgg
     1981 cgaggatgtc gttgaacacc tgctgcgcct gcttttccga aagcttcagc gcctcaccca
     2041 gttccgcggc gctgtagccg tggttgagcg cgtacagcgc gagatccatc tgcgcatacg
     2101 gcagcgcaaa gtagaactcg tcctggccct gcgccaggct gtacgtgtcg gtggtcggaa
     2161 tcgccgcgca gatttccgcg ggcagcttca tgtgccgcgc caacgcatat acctggctct
     2221 tgtagaggtg cgcgatgggt ttgacgtcgg cgctgccgtc gccgttcttc acgaagaaac
     2281 cctggtcgta ttccaggcgg ttcggcgtgc cgatcacggc gtagttcagg cgatccgcgt
     2341 ggtaatactc cacggtcttg cggatgcgct gcttgtggtt ggtcgcggcg acgatctgca
     2401 ggtattcggg cagcggcagt cgcgcggtct tcaattcacc ggcgggcgtc tgcaccacga
     2461 gctcgaagtg gttgatgcgg ccttcgagac cacccttgat gatgatcttg ttcttccagc
     2521 cgtcggtgta ctcggggaat acgcggcgaa tggcttcgtc gcgctgccgg tagcagccga
     2581 tgccttcaag cgccggcgca atgtcgaaca cttcgtggcg gatcttgagg tgttccgcga
     2641 gctgtttgcc gcgcgcggtg ctgaagcccg aagagtcgcg ctcgggcagc atgatgccgt
     2701 acaccttgcc tggtcccagc gccttgacgc acagcgccga gctcaccgag ctgtcgatgc
     2761 cgccggacat ggcgcagacg aaaccgcggc gcatgacttc cttcgacagg agctctcgca
     2821 gccgcgcgcc aatacgctcg atttccttgt cgtagtccag ttccagtacg cgagcgtcga
     2881 gctgttgctt acccatcttt attcctctca agccagtttg tagcgttgta ctttccccga
     2941 agacgtgcgc ggcaactcca ccgcgaattc cacgactttc ggaatcttgt attgggccag
     3001 gtgcgcgcgg caatgcgcct tcacccccat gacatcgggc gccgcgcctt cgcgcagcac
     3061 gatgacggcc ttgatcgcct gccccaacaa tgcgtcgggc atgccggtga ccgcgacttc
     3121 ctgcactccg ggcagcgtcg cgagcacctc ttcgatttcc tgcgggctca cgcggaacgc
     3181 gcccaccttg atcatctcca cctcgcggcc gtcgatgtag agatagccgt caccgtcgcg
     3241 atgcgcgagg tcaccggtgt gcagccatcc attgcgcacg gcctggacgg tcagcgcggc
     3301 atcgtcccaa tagcccatca tgacgttcgg tccgcgcgcg cagagctcgc cgacctcgtc
     3361 gtcgggcacc acgcggcccg aaggatcgcg aatctcgata tcgacgcccg gcagcggcaa
     3421 ccccaccgaa cccagtttct cgtcgagttc atcgggcgga aggaaggtca gccgtgcggt
     3481 ggcttcggtc tgtccgtaca tgacgaacag ccgcacgtgc ggcaacgcgt cgcgcaatgc
     3541 ctggatctgc ggccgcggca tggcgccgcc ggcctgggtc acgtaccgca ggcgcgacag
     3601 gtcgaaatcg gcaagctgcg tgcggcctaa caacaatgcg taagtggccg gcacgccggc
     3661 gaaagccgtg atcgcttcgt cctgcaggcg cttcaccagc acctgcggaa aagccaggtt
     3721 gtcttcgagc acgaggtgcg cgccggccga cagattgctg ttgagcaccg acatgccata
     3781 cgaaaaatga aacggcagcg ggcacatcac cttgtcggtg ggcacgatct cgaggtagtt
     3841 cgcgatgatg cgagcgttcg agctcaggtt gccgtggctc agcatcacgc ccttcgggcg
     3901 accggtggtc cccgaggtgt agatgatgca ggcgagcgtg tcgggcgtaa tcgtcagcgc
     3961 ctcgcgcggc ggatgggctc ccagacttgc gcagaacgcc tcgcaaccat tgggcgcatc
     4021 cacgagctcg agcggcagca gcgtcacgtc ccaacccgag agcgcgtcac gcagcgccgc
     4081 gaactccacg tgcgcggcgt cggcgagcac cacgcgcgcg ccgcaatgtt cgatctgccg
     4141 ggcgagcacg ccggcgcgtt cctgcacatt gagcggcacg gcggcggcgc ccgcacggag
     4201 cacaccgtaa aacgccgcgg cgaactgcgg cgaattgcgc agcagcaacg ccacgcgatc
     4261 acccgtgttc accggcaacg catcgctgac gcgggctatg gcgcccgcga ggtccgcgta
     4321 gctccaggtc gccttgtgcc acgacaacgc gggcgcgccg gcattgcggc gtgcagcgtc
     4381 ctcgatggcg gcgacgagag tggtcattcg tggattgaca gcgtgggctc aggcagcctt
     4441 gcgcttgttg gcgacaaagc ccgcgatctt gttcacgccg tcgagattgt cgggaatcaa
     4501 ttcctcgtcc ttca
//
LOCUS       DATDEU010000471         3337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1693445, whole genome shotgun sequence.
ACCESSION   DATDEU010000471 DATDEU010000000
VERSION     DATDEU010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693445"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1981
                     /locus_tag="VIV63_11245"
     CDS             <1..1981
                     /locus_tag="VIV63_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6125217.1"
                     /translation="SITFLCDRAEMGQGVYTALPMLIAEELGVSLARIKVEFAPPGDA
                     FINNLIGGQITGGSTSVRDGWEKLRKAGATARMLLVSAAAEEWGVDARSCKVADGVIL
                     SPQFKKLTFGEVAEAAAKLPVPKDVPLKPASQFTLIGKPQRRIDTPSKVDGSAVYGID
                     VKLPGMLHAALLQSPTLGGTVKTFNDDKARAMPGFVATVLTSSGVAVVADSWWRARKA
                     RDVLVVEWDAGPNAALNDARIMQTLRKGAAGPGRVARHDGDAAAAIKGAARVVRADYE
                     LPLLAHATLEPQNCTADVRDDGADIYVPTQVQQIAQATAAKAAGLDPKLVKVHTTFLG
                     GGFGRRLEVDFIPAAVEASKALKKPVKLVWTREDDMTHDAYRPPAFDQVAGGFDEKGK
                     LVAFNLHLTGPSITARMFPSVTEKMVDPFAIEGAANYPYDVPNVHVDFQQQEIGINVG
                     YWRSVSHALNCFVVESFMDELAVATKKNAYEFRKALLEKQPRFLKVLDLVVKESRFGH
                     AQRGHFHGLSVMEGYGTYMAQVAEVSIDVGENAGKIKVHRVYCAADCGQQVNPDTVVA
                     QIESSIIFGLSALLWGEINIQNGRVQQTNFDNYKVARMTDAPRIDSYVIESGEAPGGI
                     GEPATALVAPAVCNAIYTATGRRLRSLPLSRHRLA"
     gene            1991..3292
                     /gene="gabT"
                     /locus_tag="VIV63_11250"
     CDS             1991..3292
                     /gene="gabT"
                     /locus_tag="VIV63_11250"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY6125218.1"
                     /translation="MSKNADLHKRRIAAIPRGVGNSLSVYAERARNAELWDVEGRRYV
                     DFASGIAVVNTGHVHPHVKAAMAAQLEKITHGCFQVTPYESYIALAEALNKLAPGPTP
                     KKTIFLSTGAEAVENAIKIARYATKRSAIIAFSGGFHGRTLGCIALTGKVQPYKAGFG
                     PMLPEVFHVPYPMAYHGVTPEDSFAAIDHLFKADVDPARVAAIIIEPVQGEGGFYIAP
                     PEFLRGLRALCDKHGILLIADEIQTGFARTGRTFAIEHSGVEPDLMTVAKSIAGGVPL
                     SAVIGKAEIMDAPVVGGLGGTFAGSPLACAAGLAVLEVLEKEKLNQRAEQMGAKLTTR
                     LKQMQAKFNCIGDVRALGMMVAMELVKNRRADAPDAELTKALVQAAGRRGLILLSCGI
                     YSNVIRILAPLTIPEEQLEEGLGLLEQSLVEATQGAATAVA"
BASE COUNT          618 a         1091 c         1092 g          536 t
ORIGIN      
        1 cagcatcacc ttcctctgcg atcgcgcgga gatgggccag ggcgtctaca ccgcgttgcc
       61 catgctcatc gccgaagaac tcggcgtgag cctcgctcgc atcaaggtcg aattcgcgcc
      121 cccgggtgat gcgttcatca acaacctcat cggcggacag atcaccggcg gcagcaccag
      181 cgtgcgcgac ggctgggaaa aacttcgcaa ggccggcgcc accgcgcgca tgttgctggt
      241 cagcgccgcc gcggaggaat ggggcgtcga tgcccgatcc tgcaaggtcg ccgacggcgt
      301 catcctgtcg ccgcaattca agaagctgac gttcggcgaa gtcgccgaag cggccgccaa
      361 actacccgtg ccgaaagacg tgccgctcaa gccggcgtcg cagttcaccc tgatcggcaa
      421 gccgcagcgg cgcatcgaca cgccgtcgaa ggtcgatggc agcgcggtct acggcatcga
      481 cgtgaaactg cccggcatgc tgcacgcggc gttgctgcag tcgccgacgc tgggcggcac
      541 ggtgaaaact ttcaacgacg acaaggcgcg ggccatgccc gggttcgtcg ccaccgtgct
      601 gacctcttcg ggcgtcgccg tggtcgccga ttcgtggtgg cgcgcccgca aggcgcgcga
      661 tgtgctcgtc gtcgaatggg atgcgggtcc gaatgccgcg ctcaatgacg ccaggatcat
      721 gcagacgttg cgcaagggcg ccgcggggcc gggccgagtg gctcgccatg acggcgacgc
      781 ggccgcggcg atcaagggcg ccgcgcgcgt ggtgcgcgcc gactacgaac ttccgctgct
      841 cgcgcatgcc acgctcgagc cgcagaactg cacggccgac gttcgcgacg atggcgccga
      901 catctacgtg cccacgcagg tccagcagat tgcgcaggca acggccgcca aggccgcggg
      961 cctcgatccg aagctcgtga aagtgcacac cactttcctc ggcggcggtt tcggccgacg
     1021 tctggaagtg gacttcattc ccgccgcggt cgaagcttcg aaggcgctca agaagccggt
     1081 gaagctcgtg tggacgcgtg aagacgacat gacgcacgac gcgtatcgtc cgccggcctt
     1141 cgaccaggtc gcgggcggtt tcgacgagaa gggcaagctg gtggctttca acctgcacct
     1201 cacgggcccg tcgatcaccg cgcgcatgtt cccctcggtc accgagaaga tggtggatcc
     1261 gttcgccatc gagggggccg ctaactaccc gtacgacgtg cccaacgtgc acgtcgattt
     1321 ccagcagcag gaaatcggca tcaacgtcgg ctactggcgt tcggtgagcc atgcgctcaa
     1381 ctgtttcgtg gtcgagagct tcatggacga actggccgtg gccaccaaga agaacgccta
     1441 cgaattccgc aaggcgctgc tcgagaaaca gccgcgtttc ctcaaggtgc tcgacctcgt
     1501 ggtcaaggaa tcgcgttttg gtcatgcgca gcgcggccat ttccacggac tctcggtcat
     1561 ggagggctac ggcacctaca tggcgcaggt cgccgaggtc tcgatcgacg tgggcgagaa
     1621 cgccggcaag atcaaggtgc atcgcgtcta ttgcgccgcg gactgcggcc agcaggtgaa
     1681 ccccgacacc gtcgtcgcgc agatcgaaag ctcgatcatc ttcggcctgt ccgcgctcct
     1741 gtggggcgag atcaacatcc agaacggccg cgtgcagcag acgaatttcg acaactacaa
     1801 ggtggcgcgc atgaccgatg cgccgcgcat cgactcctac gtgatcgaga gcggcgaagc
     1861 cccgggcggc atcggcgaac cggccactgc actggtggct ccggccgtct gtaatgcaat
     1921 ttatacggcc accgggcgta gacttcggtc actccccttg agcaggcacc gattagccta
     1981 gaggcccgtc atgtcgaaga atgccgatct ccataagcgc cgcatcgcgg ccattccgcg
     2041 tggcgtgggc aattcgctca gcgtttatgc cgagcgcgcg cggaacgccg aactgtggga
     2101 cgtcgaaggc cgccgctacg tggacttcgc gagcggcatc gccgtggtca acacaggtca
     2161 cgtgcatcct catgtgaagg cggcgatggc cgcgcagctc gagaagatca cgcacggctg
     2221 tttccaggtg acgccgtatg aaagctacat cgcgctggcc gaagcgctga acaagctcgc
     2281 gcccgggccc acgccgaaga agacgatctt cctgtcgacc ggcgcggaag ccgtggagaa
     2341 cgcgatcaag atcgcgcgtt acgcgacgaa gcgctcggcg atcatcgcct tcagcggcgg
     2401 gtttcatgga cgcacgctcg gctgcatcgc gctcaccggc aaggtgcagc cgtacaaggc
     2461 gggcttcggg cccatgctgc cggaagtgtt ccacgtgccc taccccatgg cctatcacgg
     2521 cgtgacgccc gaggattcgt tcgcggccat cgaccatctg ttcaaggcgg atgtggatcc
     2581 ggcgcgggtc gccgccatca tcatcgagcc cgtgcagggc gaaggcggct tctacatcgc
     2641 gccgccggaa ttcctgcgcg gcttgcgcgc gctgtgcgac aaacacggca tcctgctcat
     2701 cgccgatgaa atccagaccg gcttcgcgcg caccggcagg acgttcgcca tcgaacattc
     2761 cggcgtggag cccgacctca tgacggtcgc caagagcatt gccggtggtg tgccgctgtc
     2821 ggcggtaatc ggcaaggcgg aaatcatgga cgcgcccgtg gtcggcggac tcggcggcac
     2881 attcgccggc tcgccactcg cctgcgccgc gggcctggcc gtgctcgaag tgctggagaa
     2941 ggaaaaactg aatcagcgcg cggagcagat gggcgcgaag ctgacgacgc ggctcaagca
     3001 gatgcaggcc aagttcaact gcatcggcga tgtgcgcgcg ctcggcatga tggtggccat
     3061 ggaactcgtg aagaaccgtc gcgccgacgc gcccgatgcc gaactcacca aggcgctggt
     3121 gcaggccgcc ggacgccgcg ggctgatcct gttgtcttgc ggcatctact cgaacgtcat
     3181 ccgcatcctc gcgccactca cgattcccga agagcagctc gaggaaggat tggggttgct
     3241 cgagcagtcg ctcgtcgaag cgacccaggg cgccgccacc gccgttgcct gaaaagagga
     3301 caggcctgcg aaaggcgccg cggcgcatca agacctg
//
LOCUS       DATDEU010000472         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_528195, whole genome shotgun sequence.
ACCESSION   DATDEU010000472 DATDEU010000000
VERSION     DATDEU010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528195"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VIV63_11255"
     CDS             <1..1250
                     /locus_tag="VIV63_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6125219.1"
                     /translation="VPQPPTRPGDKPDFSYVELSPAGALNKPDVNARARDMEECATGL
                     VRVLDDKHEALGQWNPRLEAEDLQVGLRHMLLTRIFDDRMQRTQRQGKISFYMRCYGE
                     EAVGVAQAMALQPGDMLFPSYRQQGIYMVRGKPLVELMCQLLSNTRDMCKGRQLPVMY
                     HWNAGRIFSISGNLTTQFPQAVGWAMAAAIRNEDHIACTWIGEGSSAEADFHHGIIFA
                     AVHQAPVIMNIVNNQWAISTFQGTAGGEQRSFAARGPGYGIAGIRVDGNDVLAVYSVT
                     QWAAQRARTGGGPTLIELVTYRASAHSTSDDPSRYRPKDDYEHWPLGDPVDRLKNHLI
                     ARGEWSEKRHKELHAELDAQVVTAWKEAVSFGALNEGPRLDRHLMFEDVYKEFPENLR
                     RQSEQLAEEIKLNAELGLAAKVE"
     gene            1253..>2255
                     /locus_tag="VIV63_11260"
     CDS             1253..>2255
                     /locus_tag="VIV63_11260"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY6125220.1"
                     /translation="MAQMNMIQALNSAMDVMMARDPSVVVMGEYVGYFGGVFRVTDGL
                     QRKYGDHRVLDSPISEGGIVATAIGMAVNGLKPVAEIQFADYIYPAVDQIASELARIR
                     YRSAGDFSSPVVIRAPCGGGIRGGQTHSQSPEAIFTHICGVKVVMPSNPYDAKGLLIA
                     AIEDDDPIIFFEPKRIYNGPFDGDPNKPAVPWSTHAKGNVPEGHYKVPIGKCDIVRPG
                     EQVTIVTYGTMVYVCEAAARKLDIDAEIIDVRTMSPLDIDPICSSVKKTGRCVVAHEA
                     TRFSGFGAELSASLQEECFWYLEAPIQRVAGWDTPYPHAFEWEYFPGQARVALALRAA
                     "
BASE COUNT          437 a          741 c          712 g          365 t
ORIGIN      
        1 acgtacccca gccacccacg cgaccgggtg acaagcccga cttcagctat gtcgagctgt
       61 cgcccgccgg cgcgctgaac aaacccgacg tcaacgcccg cgcccgcgac atggaagagt
      121 gcgccacggg actggtgcgc gtgctcgacg acaaacacga ggcgctgggc cagtggaatc
      181 cgcggctcga ggccgaagac ctgcaggtgg gcctgcgcca catgctgctc acgcgcatct
      241 tcgacgaccg catgcagcgc acgcagcgcc agggaaagat ctccttctat atgcgttgtt
      301 atggcgagga agccgtgggc gtcgcccagg ccatggcgct gcagccaggc gacatgctgt
      361 ttccgtccta ccggcagcag ggcatctaca tggtgcgcgg caaaccgctg gtcgaactca
      421 tgtgccagct gctgtcgaac acgcgcgaca tgtgcaaggg ccggcaactg cccgtgatgt
      481 accactggaa cgcgggccgc atcttctcga tttccggcaa cctaactacc cagtttcccc
      541 aggccgtggg ctgggccatg gccgcggcca tccgcaacga agaccacatc gcctgcacct
      601 ggatcggtga aggctcgagc gccgaggcgg atttccacca cggcatcatc ttcgcggccg
      661 tgcaccaggc gccggtcatc atgaacatcg tcaacaacca gtgggccatc tccacgttcc
      721 agggaacggc gggcggcgag cagcgttcgt tcgcggcgcg cggcccgggt tacggaatcg
      781 cgggcatccg cgtcgacggc aacgacgtcc tcgccgtgta ttcggtcacg cagtgggcgg
      841 cgcagcgcgc gcgcaccggc ggcgggccca cgctcatcga gctcgtcacc taccgcgcgt
      901 ccgcgcattc caccagcgac gatccgtcgc gctatcggcc caaggacgac tacgagcact
      961 ggccgctcgg cgacccggta gatagattga agaatcacct gatcgcgcgc ggcgaatgga
     1021 gcgagaagcg gcacaaggaa ttgcacgccg agctcgatgc gcaagtggtc accgcgtgga
     1081 aagaggccgt gtcgttcggt gcactcaacg aagggccacg gctcgatcgt cacctgatgt
     1141 tcgaggacgt ttacaaggag ttcccggaga acctgcgccg gcagagcgag cagctcgccg
     1201 aggaaatcaa actcaacgcc gagctcgggc tcgcggccaa ggtggaataa ccatggccca
     1261 gatgaacatg atccaggcgc tgaactccgc catggacgtg atgatggcgc gcgatccgtc
     1321 ggtggtcgtg atgggtgaat acgtcggcta tttcggtggc gtgttccgcg tcaccgacgg
     1381 cctgcagcga aagtacggcg atcaccgcgt gctcgattcg ccgatctccg aaggcggcat
     1441 cgtcgccacg gccatcggca tggcggtcaa tggcctcaag cccgtcgccg aaatccagtt
     1501 cgcggactat atctatccgg cggtggacca gatcgcgagc gagctcgcgc gcatccgcta
     1561 tcgcagcgcc ggcgatttct cttcgcccgt cgtcatccgc gcgccctgcg gcggcggcat
     1621 tcgcggcggg cagacgcatt cgcagagccc cgaagccatc ttcacgcaca tctgcggcgt
     1681 gaaagtggtg atgccgagca atccctacga tgcaaagggg ctgctcatcg cggccatcga
     1741 agacgatgac ccgatcattt ttttcgaacc gaagcgcatc tacaacgggc cctttgacgg
     1801 cgaccctaac aagcccgccg taccctggag cacgcacgcg aaaggcaacg tgcctgaagg
     1861 ccactacaag gtgcccatcg gcaagtgcga catcgtgcgc ccgggcgagc aggtgaccat
     1921 cgtcacctac ggcacgatgg tgtacgtgtg cgaagcggcc gccaggaagc tcgacatcga
     1981 cgccgagatc atcgacgtgc gcaccatgtc gccgctcgac atcgatccca tctgctcgtc
     2041 ggtcaagaag accggacgtt gcgtggtggc gcatgaagcc acgcgcttct ccggcttcgg
     2101 tgcggaactc tcggcgagcc tccaggaaga atgtttctgg tacctcgaag cgccgatcca
     2161 gcgtgtggcc ggttgggata cgccatatcc gcacgcgttc gaatgggagt attttcccgg
     2221 gcaggcacga gtggcgctcg ctctgcgagc agcca
//
LOCUS       DATDEU010000473         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2131566, whole genome shotgun sequence.
ACCESSION   DATDEU010000473 DATDEU010000000
VERSION     DATDEU010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131566"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VIV63_11265"
     CDS             <1..1323
                     /locus_tag="VIV63_11265"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6125221.1"
                     /translation="VAMCDVDWAFVDARYADIPKQLEAANKRLAEATDPKQKTQIQAQ
                     ITGWQQLQEKWPKAKRYTDYREMLAKQKNIDAVVVATPDHTHAHIALAAMDLGKHVYV
                     QKPLAWSAEECRRLAAKATATKLQTQMGNQGHSSDDARVVNEYIQSGAIGTVTEVHVW
                     TNRPLAYWPQGIPRPAPLPPNAKDLPWNMNGVMTRAAGSFGTYAPPDKLAWDLFLGPS
                     RYIDYHPIYHPFNWRGWTDWGVGAIGDMGAHLIDSSFWSLDLDYPTSIETTSTPYNKD
                     TYPMASTTYYEFAAKGSRPAVKMTWYDGGLLPAKPVEMGDEELNKGGGVLYIGTRGKL
                     LHDTYGFNPRLLPKSLHESVGKPKETYARITTSHEMNWIDAIRGTQKTTSPFEYSGKL
                     TEIMLLGVAALNAGKKILYDGANMKITNVPDSDALLRRQYRNGWELPA"
     gene            1492..2325
                     /locus_tag="VIV63_11270"
     CDS             1492..2325
                     /locus_tag="VIV63_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125222.1"
                     /translation="MKWTALVAASAISLASAVAGAAPAKSATIEKSPDVLRAPQVRKS
                     AARASTPLLLAPPITAAAAPTVEDVGDADSFGRNVTYLGLAQTLSVTVQPDCTGSDPT
                     LERCVVANPAPAPTSFNEAGLATINLPARATKSLVCFALTPFIQIQWENNLATPATAR
                     FNANALITIDNEVLDDPTLIDPGTGLPFGGVLSLGLGTFTDFHTLQPAETDFKALQMS
                     RSCIAGIISKQNLIEGYGLTETQATQFFKKPMAITFGARGTVGLSAFANYFYGIRLYG
                     D"
     gene            2450..>2865
                     /locus_tag="VIV63_11275"
     CDS             2450..>2865
                     /locus_tag="VIV63_11275"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY6125223.1"
                     /translation="MRIGKLLAGILPLVFAATGVVSCVTQPFVRAGASTEVDVDARAL
                     ESHVRMLAVTLHPRSVDNPPNLDRAADYVLAQFNATGAATEQQLVEADGRQFRNLIAR
                     FGPLDGPVVVIGAHYDSCGDTPGADDNASGVAALLEL"
BASE COUNT          557 a          995 c          840 g          473 t
ORIGIN      
        1 gtcgccatgt gcgatgtgga ctgggccttc gtggacgcgc gttacgccga catccccaaa
       61 caactggaag cggctaacaa gcgcctggcc gaagccaccg accccaaaca gaaaacgcag
      121 atacaggccc agatcaccgg ctggcagcaa cttcaggaga agtggccgaa ggccaagcgc
      181 tacaccgact atcgcgagat gctggccaag cagaagaaca tcgacgccgt ggtggtggcc
      241 acgccggatc acacacatgc gcacatcgcg cttgcggcca tggatctcgg caaacacgtg
      301 tacgttcaga aaccgctggc gtggtccgcc gaggaatgcc ggcggctcgc ggcgaaggcg
      361 accgccacga aattgcagac gcagatgggc aaccaggggc actcctccga cgatgcgcgc
      421 gtcgtcaatg aatacatcca gtccggggcc atcggtaccg tcaccgaggt gcatgtctgg
      481 accaaccggc cgctggccta ttggccgcag ggcattccgc gccccgcccc gttgccgccc
      541 aacgccaagg acttgccgtg gaacatgaac ggcgtgatga cgcgcgccgc cggttctttc
      601 ggcacctacg cgccgcccga caaactcgcc tgggatctct tcctgggccc gtcgcgatac
      661 atcgactatc accctatcta ccacccgttc aactggcgcg gctggaccga ctggggtgtc
      721 ggcgccatcg gcgacatggg tgcgcatctc atcgattcgt cgttctggtc gctggatctc
      781 gactacccga cgtccatcga gaccacgtcc acgccttaca acaaggacac ctatccgatg
      841 gcgtccacca cctactacga gttcgcggcg aagggctcgc ggcccgcggt gaagatgacc
      901 tggtacgacg gcgggctgct gccggcgaag cccgtggaga tgggtgacga ggagctcaac
      961 aagggtggcg gcgtgttgta catcggcacg cgcggcaaac tgctgcacga cacctatggc
     1021 ttcaatccgc gcctgctgcc gaagtcgctg catgaaagcg tcggcaagcc caaggaaacc
     1081 tacgcgcgca tcaccaccag ccacgagatg aactggatcg acgccatccg cggtacgcag
     1141 aagaccacga gcccgttcga atactcgggc aagctcaccg agatcatgtt gctgggcgtc
     1201 gcggcgctca acgccggcaa gaagatcctg tacgacggcg ccaacatgaa gatcaccaac
     1261 gtgccggact ccgacgcatt gctacggcgc cagtaccgca atgggtggga actccccgca
     1321 tgaaccctac cgcgagcgtg ccatgagcac gctgcgcggc cggatctttc accgggaata
     1381 agtctcaact ttcgacacca gcttgtgcgg gcgctacgcc tgcgcgtgtt cctccgatta
     1441 agtttcggca gcggcgcttt tcgcaccgcc gaagtttcaa ggaggaacat gatgaaatgg
     1501 actgcactgg ttgccgcgtc ggcaatctcg cttgcttcag ccgtcgcggg agctgctccc
     1561 gccaaatccg ccaccatcga gaaatcacca gacgtcctgc gtgccccgca ggtccgcaaa
     1621 tccgcggcac gcgccagcac accgctgttg ctcgctccgc cgatcaccgc cgccgcggcg
     1681 cccaccgtcg aagacgtcgg tgatgccgac tctttcggcc gcaacgtcac gtacctcggc
     1741 ctcgcacaga cgctgtccgt gaccgtccag cccgactgca ccggcagcga tcccacgctc
     1801 gagcgctgtg tcgtcgcgaa tcctgcgccc gcgcccacgt cgttcaacga agccgggctc
     1861 gccaccatca accttccggc cagggccacg aagtcgctgg tgtgtttcgc gctcacgccg
     1921 ttcatccaga tccagtggga gaacaatctc gcgacaccgg ccacggcgcg cttcaacgcc
     1981 aacgcgctca tcaccatcga caacgaagtg ctcgacgatc cgacgctcat cgatccgggt
     2041 acgggcctgc ccttcggcgg cgtgttgtcg ctcggcctcg gcacgttcac cgacttccac
     2101 acgctgcaac ccgccgagac cgacttcaag gccctgcaga tgtcgcgttc ctgcatcgcc
     2161 ggaatcatca gcaagcagaa cctgatcgaa ggttacggac tgaccgaaac ccaggcgacg
     2221 cagttcttca agaagcccat ggccatcacg ttcggtgcgc gcggcacggt cggcctctcg
     2281 gcgttcgcca actacttcta cggcatccgc ctgtacgggg attgaccgca aggattggcg
     2341 ccgggctctt cccgaaaact gcatccggcg ccgcggagct tcgcgtgact caaccccatg
     2401 cgaagcccct tttttagtag acgataagag ttagagtgcg cctcacaaga tgcgcatcgg
     2461 gaaactcctc gccggaattc tgccgttggt cttcgccgcg acgggcgtcg tatcctgcgt
     2521 gacccagcct ttcgtacgcg cgggggcatc caccgaggtc gacgtcgatg cacgtgcgct
     2581 cgagagccac gtgcgcatgc tggcggtcac cctgcatcct cgcagtgtcg acaacccgcc
     2641 caacctcgac cgcgccgcgg attacgttct cgcgcaattc aacgccaccg gcgctgccac
     2701 ggagcagcaa ctcgtggaag cggacggccg ccagttccgc aacctgatcg cccgcttcgg
     2761 cccgctcgat ggcccggtgg tggtcatcgg tgcgcactac gattcctgcg gcgacacgcc
     2821 gggggccgac gacaatgcga gcggggtcgc ggcgctgctc gaact
//
LOCUS       DATDEU010000474        12750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1985843, whole genome shotgun sequence.
ACCESSION   DATDEU010000474 DATDEU010000000
VERSION     DATDEU010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985843"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /gene="nagA"
                     /locus_tag="VIV63_11280"
     CDS             <1..1121
                     /gene="nagA"
                     /locus_tag="VIV63_11280"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEY6125224.1"
                     /translation="NGRMLTDAGIETGRNLLVRDGMIEGVVGAREIVGADRTIDLEGQ
                     LLVPGFIDTQVNGGGGGLFNDEPSVEAIAAIGQAHRRFGTTGYLPTLISDDLSVVASA
                     IDAVREAITRGVPGVLGIHIEGPFLSNERRGVHDASKLRTLDEDAMKLLVTPHGGVTM
                     VTLAPERTTPAIIGALSDAGVIVSAGHTNATYDELQPALEAGVRGFTHLFNAMSQLGN
                     RDPGAVGAALAHADSWAGLIVDGHHVHPEVLKIALRAKRLDRFMLVSDAMPSVGADVR
                     EFQIQGRLITVQGDKIVDDEGRLAGAHLDMAGAVRNTVELLGLPLTDALRMASTYPAE
                     FLKLQGRVGRIAQGQRADLVLLDESLDVVESWIAGRAD"
     gene            complement(1118..2005)
                     /locus_tag="VIV63_11285"
     CDS             complement(1118..2005)
                     /locus_tag="VIV63_11285"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY6125225.1"
                     /translation="MADIIIIGPGAIGTTLTAWLSQNSGHRVSVGVRTPFRSIVLNTP
                     GGRIEARPEVLADPSQGRPVDWVLVTTKAYDSAAAARWFDKSFGEHTRLAVIQNGVEH
                     VERFTPHVDAARIVPVMIDCPATRHAPGEVTQGGPIHIVVPEGANGAAFVELFAGLNL
                     DVRETPDFVTAVWKKLCLNSAGAVSAVLLEPAGIAHHDGVAEILRTIVRECIAVGRMR
                     GAVLADEIAADIVDRMRRGPRDASNSMLADRRAGRPMEIDARNGVIVRLGREYGIPTP
                     MNALMVAMLESAQDLQIGS"
     gene            complement(2046..2270)
                     /locus_tag="VIV63_11290"
     CDS             complement(2046..2270)
                     /locus_tag="VIV63_11290"
                     /inference="COORDINATES: protein motif:HMM:NF022152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HEY6125226.1"
                     /translation="MTELLGWVATAVFVGSYFFTRPALLRAAQMAGATLWIVYGAMIG
                     AIPVVVANGLVFAAAAWTLFRARQQPLTQV"
     gene            complement(2281..3096)
                     /locus_tag="VIV63_11295"
     CDS             complement(2281..3096)
                     /locus_tag="VIV63_11295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY6125227.1"
                     /translation="MNKRPAKHFLLASDFDQTLSFHDSGFELSDLLGVTNFEQKVAGL
                     SASHLVQQGGELAYLIRHDPEFRGVRREHLIEAGKRVRLKKGLPAFVEFLDRGIDGNQ
                     FSFYVVSAAPREVVVSALEGIVPADHIFGTELDYDPQSGEVRSIGRVRAGYGKVAVLE
                     ELGSKLDITSDRVIYVGDGSSDIHVMLHVNNGDGFTIAVSENRQLARIAQSTVLSDNA
                     SSVLVPVLDQVLGLRTGEIRALLESTGLVLDDWQRARTDRVLIREWAPLASVG"
     gene            3394..5034
                     /locus_tag="VIV63_11300"
     CDS             3394..5034
                     /locus_tag="VIV63_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY6125228.1"
                     /translation="MLYVAGISMGIAHAQTLESPPSAPSELQTQIEALVESGPFEIHG
                     AHIAYPNVIHAFYSQRTFRPAWADAKTASELRRALKDSEADGLDPNDYHLPVLEQLSG
                     PSATGAPGNSSTQAALEILYTDALLRIADHLMFGKVDAASFDAHWNYTRTPTGIDVPQ
                     RIERALASGDLYAEIEKLKPTHRMYRTLKQELARYRQAAVEPRRIVIAAGKTLEPDTH
                     DERVLALRTRLAISGDIDAPQDSASDLYDATLLAAVRRYQERMGILADGKIGARTITE
                     LNVPIEERITQLLVNLDRGRVLLHDLPNEFVVVNIAAYWVYLVRGQEIIWSARTQVGK
                     TYRRTPMFRSAIDYVVFNPTWTVPPGIIRNDILPEARRDPASITRRGLNVLDAQGNKV
                     DPASVDWSRFKSGNIPYTLRQDPGPKNALGRVKVMFPNSYSVYLHDTPSQSKFEETDR
                     AFSSGCVRTERALELAELLLANPASWNPATVQRTIDAGTTQNVTLPNKVPVLLAYWTA
                     WVDPQGRVNFRRDIYGQDAQWAAALTRPYSPRKIPQLP"
     gene            complement(5031..7289)
                     /locus_tag="VIV63_11305"
     CDS             complement(5031..7289)
                     /locus_tag="VIV63_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY6125229.1"
                     /translation="MQSSRRLRCIALLAGALQVLSPLAALGADATIASGRPRIGLVLA
                     GGGAKGGAHVGVLKVLEELHVPIDCIAGTSMGALIGGGYAAGIPADDLEKFLISIDWK
                     RVVGSQGRRDLEPIEQKRSGPTYSNEFEFGITPDGVAAPAGLINTSNVEDLLRVYVAR
                     ARLETDFNKLPIPYRAIATDMVSSKMVVLDQGDLATAMRASMAIPGGFAPVMLDDMIL
                     SDGGLVRNIPIDIARNLCADQVIVVNLVEPEANPKKLQSATQLLGRAMDVMIIANEEL
                     QLASLRPGDVRIDVIMGDITTADFERVPETVPLGVDAARKMAGALSQFSVPEKQYVAW
                     RTSVTQSQKIEGKLAGVRYEGLERVNPDYLAHRQKVQPGDPVDIVTISEEAQRLSALQ
                     DFDSVSYRLDGERDSPTLTWLPQEKKFGPNYLRVDLGAYASREGDVTFALYGRHVRTW
                     LNSLGGQWFNELQLGGESLLTTGLYQPLDSAHRFFIEPRVAITRSLEDVFSMDERVAR
                     YEFRDFVGEFNFGANVGRYGQARIGYLYDEREIEVDIGSSLMPEGDPVDAGMHVLAEL
                     DSRDTAFNPTRGFAAAVDYMKSDSSLGADRDWEKAEIGLGMALPLRRDVLWMTVAAGS
                     DFDSDLPGDRMFSVGGPDSFPGFEQGELRVREYWLASTSYLWRVKDLLPIRNLSLYMG
                     FGLTGGEVFDRLDGGEDGELYGGSVFLTGRTLFGPLTVGVGTTSTDSWSLWLSVGRPV
                     GHGTILERGVCR"
     gene            complement(7370..10093)
                     /locus_tag="VIV63_11310"
     CDS             complement(7370..10093)
                     /locus_tag="VIV63_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6125230.1"
                     /translation="MRLNVFKNFRLSLQLYALVAITLAIATGLIFYAMQQVKSTQGTL
                     KHTIDNRMVSGQSIQGVADALSLSLEASLDVIEKKVTPIDAHDQIKKAIDKARDDWDN
                     YFLGDMITEEQELADETTPMLDGAYGKIDKLLKKLETNDIADLAAWRNETLRPALADG
                     ASNLKKLIAMQLTAANLDLDNANKNYDLALRNGKVLLGAGALLAILMAWFIIRSAMRK
                     LGADPAVAAQVARRVASGELEFEMLPGRHDAISLMGALRQMKDSLLHSKLDYQGQINA
                     IAKVQGVIECSLEGDVSNANDIFLKLLGYSLEEVQGKNYSMFADDAKELWPALKRGES
                     RQGEYRVVSRDKREIWFQGVFNPIVDASGKPFKVVAYLNDVTKQRQEALLNAAFRGAL
                     DRLDANVMCADNDMKIIFVNPAAAQMLTRAQESFRKELPGFDAGKLLGASVESMTRDP
                     AALRGVIERLTDIVTRQEVIGGRSMKTIMSPMKDDTGRRLGTVLQWFDRTQEVATETE
                     LQQVIGAVTAGNLENRISLEGKRDFFLTLSGGINELVDAIATVVAEVRGVVAAANQGD
                     LTRRIDIKDKAGLLVQIGSGINDLTSTLSALVSQVKTASAEVSRGADEISQGNANLSQ
                     RTEEQASSLEETASSMEEMTSTVKQNADNAAHASKLAVAARDQAEKGGAVVSKAVDAM
                     VDINGASKKIADIISVIDGIAFQTNLLALNAAVEAARAGEQGRGFAVVATEVRNLAGR
                     SAAAAKEIKGLIQDTVTKVDEGSKLVTQSGATLEQIVAAVKKVTDIVSEIAAASHEQS
                     AGIDQVNKAVMQLDELTQQNAALVEQASAASQAMADQARELNSSMTRYQVNATGASAA
                     APEVAPSQTPADRRKSSRPWNSKVAAPAAEPKARASARVANSDADMEWKEF"
     gene            complement(10117..10632)
                     /locus_tag="VIV63_11315"
     CDS             complement(10117..10632)
                     /locus_tag="VIV63_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY6125231.1"
                     /translation="MQNKDSREAAGAGGTTTRQVLTFSLGKEVYGVDILRVKEIRGWS
                     PVTRMPQSEASMLGVLNLRGLIVPVIDLRVRFALESAAFTPLTVIIVLSLRTANGQRE
                     CGIVVDNVNDVVDIDAASIKPAPAMSGADSSEFIEGIATHDERMLILLHAESLASSEQ
                     TSIGLASAAAA"
     gene            10811..11092
                     /locus_tag="VIV63_11320"
     CDS             10811..11092
                     /locus_tag="VIV63_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125232.1"
                     /translation="MKVLVSMTVLWAALAVPALACERPSAPTSIPDGASASKEDMLAA
                     KKAVDAFKSGMEEYIACEKSGAKKDGAAAELVKVADRFNAQVKAFKAKG"
     gene            complement(11157..11723)
                     /locus_tag="VIV63_11325"
     CDS             complement(11157..11723)
                     /locus_tag="VIV63_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY6125233.1"
                     /translation="MKSKLSTLMFAAVMLLAGVTASADAFDDTVQIFMNAGESSAFFG
                     PSYGYAVFPNVGKGGLGVGAAHGSGRAYIHGKHVGDVKMNQLSIGLQAGGQAFSQIIF
                     FQDKRAFDEFASGTFEFDATVQAVAITASATASAGTAGAAAGAAGGKKDAATTGNWHK
                     GMAVFTVAKGGLMYQAAVGGQKFSYKAL"
     gene            complement(11807..11882)
                     /locus_tag="VIV63_11330"
     tRNA            complement(11807..11882)
                     /locus_tag="VIV63_11330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11847..11849),aa:Thr,seq:tgt)
     gene            complement(11924..12475)
                     /locus_tag="VIV63_11335"
     CDS             complement(11924..12475)
                     /locus_tag="VIV63_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125234.1"
                     /translation="MPVSPKVNAPPLELVRESRPAAVGDGGCVTVGPFATNELAARAR
                     STLADSGYTSLPREVETRDLEGYWVYLESPPTETGERRLLDRLKKGGITDAQAVGDAG
                     SRRISLGVFSDEGRAVAQSERVARLQLLPQIEARERSGTAIWLDLTLKSDSPPLEGQK
                     FPAGDTELEFRACPPAEETEAAQ"
BASE COUNT         2265 a         4122 c         4050 g         2313 t
ORIGIN      
        1 gaaacggaag aatgctgacc gacgcgggca tcgaaaccgg ccgcaatctg ctggtgcgcg
       61 atggaatgat cgagggcgtg gtcggcgcgc gcgagatcgt cggcgccgat cgcaccatcg
      121 acctcgaagg acaactgctc gtaccgggct tcatcgacac gcaggtgaat ggcggcggag
      181 gcgggttgtt caatgacgag ccgagtgtcg aagccatcgc ggccattggt caggctcacc
      241 ggcgctttgg caccacggga tacctgccca cgctgatcag cgacgatctt tccgtcgtgg
      301 cgagtgccat cgacgcggtg cgtgaggcca tcacgcgcgg cgtaccgggc gtcctcggta
      361 ttcacatcga aggcccgttc ctcagcaacg agcgccgcgg cgtacacgac gccagcaagc
      421 tgcgcacgct ggacgaagac gcgatgaagc tgctggtcac gccgcatggg ggagtgacca
      481 tggtgacgct ggcgcccgag cgaaccacgc ccgcgatcat cggcgcgctg agtgacgccg
      541 gcgtcatcgt gtcggccggg cacacgaatg caacctacga cgaattgcag ccggcgctcg
      601 aggccggcgt acgcggcttc actcatctgt tcaacgccat gtcacagctc gggaaccgcg
      661 atcccggcgc cgtgggcgcg gcgctggcgc atgccgatag ttgggcgggc ctcatcgtcg
      721 acggtcatca cgtgcatccg gaagtcctga agatcgcgct gcgcgccaag cggctcgacc
      781 gcttcatgct ggtgagcgat gcgatgccga gcgtgggcgc ggacgtcagg gagttccaga
      841 tccagggccg gctcatcacc gtgcaaggcg acaagatcgt cgacgacgaa ggcaggctgg
      901 cgggcgcgca tctcgacatg gcgggcgcgg tgcgcaatac ggtcgaacta ttgggcctgc
      961 cgttgaccga tgcgctgcgc atggcgagca cgtatccggc cgaattcctg aaattgcagg
     1021 gccgcgtggg ccggatcgcg caaggtcaac gcgcggacct ggtgctcctg gacgaaagcc
     1081 tcgacgtcgt cgagagctgg atcgccggcc gcgccgacta actgccgatc tgcagatcct
     1141 gcgcggattc caacatggcc accatcagtg cgttcatggg tgtcggaatg ccgtactcgc
     1201 ggcccagccg gacgatgacg ccgttgcgcg cgtctatctc catgggacgt cccgcgcgcc
     1261 ggtcggcgag catcgaattg gaagcgtcgc gaggtccgcg gcgcatgcgg tcgacaatgt
     1321 ccgcggcaat ttcatcggcc agcaccgcgc cgcgcatgcg gccgacggca atacactcgc
     1381 gcacgatcgt gcgcaggatt tccgcgacgc cgtcgtgatg cgcgatgccc gcgggctcga
     1441 gcagcaccgc cgaaaccgca ccggcactgt tgaggcacag tttcttccag accgcggtga
     1501 cgaaatccgg cgtctcgcgc acgtcgagat tcaggccggc aaacaactcg acgaacgccg
     1561 cgccattcgc accctcgggt acgacgatgt ggataggccc gccctgcgtc acctcgcccg
     1621 gggcatgacg ggtggccgga caatcgatca tgacgggcac gatgcgcgcc gcgtcgacat
     1681 gcggcgtgaa acgctcgacg tgttcgacac cgttctggat caccgccagt cgggtgtgct
     1741 cgccaaagct cttgtcgaac cagcgcgccg cggctgcgga gtcgtaggct ttggtggtga
     1801 ccagcaccca atccacggga cgtccctgcg aagggtcggc aagcacttcg gggcgggctt
     1861 cgatgcgccc gcccggcgta ttcagcacga tggaacggaa cggcgttcgt acgccgactg
     1921 aaacgcggtg cccggagttc tgcgaaagcc acgccgtgag cgtcgtgccg atcgctccgg
     1981 gcccaatgat gatgatgtcc gccatgccgc gatggtaata cgcggacggc atcagacccc
     2041 ggtgttcaga cctgcgtgag cggctgctgc ctcgcgcgga acaacgtcca ggcggccgcg
     2101 gcgaacacca gtccattcgc caccacgacg gggatcgcgc cgatcatggc gccgtacacg
     2161 atccacaacg tcgcgcccgc catctgtgcg gcgcgcagca acgcgggacg cgtgaagaaa
     2221 taggagccga cgaaaaccgc cgtggccacc caacccagca actctgtcat ggcgttcacc
     2281 tcagcccacc gacgccagcg gcgcccactc gcggatcagc acgcgatccg tgcgcgcacg
     2341 ctgccagtcg tcgagcacca gccccgtcga ttcgagcagc gcccggatct cgcccgtgcg
     2401 aagcccgaga acctggtcga gcaccggcac cagcacgctg ctggcgttgt cgctgagcac
     2461 cgtgctctgc gcgatgcgcg cgagctggcg gttctcggag accgcgatcg tgaatccatc
     2521 gccattgttc acatggagca tcacgtgaat gtcggagctt ccgtcgccga catagatgac
     2581 gcgatccgag gtgatgtcga gcttgctgcc cagttcctcg agcaccgcga ccttgccgta
     2641 tccggcgcgt acgcggccga tcgagcggac ctcaccggac tgcgggtcgt agtcgagctc
     2701 ggtgccgaag atgtgatccg cgggcacgat gccttcgagc gcggagacca cgacttcacg
     2761 cggggccgcg gacacgacgt agaaagaaaa ctggttgcca tcgatgccgc gatcgaggaa
     2821 ctcgacgaag gcgggtaatc ccttcttgag ccgcacgcgt ttgccggctt cgatgagatg
     2881 ttcgcggcgc acaccgcgga attccggatc gtgccggatc aggtaggcga gttcgccgcc
     2941 ctgctggacg aggtgactgg ccgaaaggcc ggcgaccttc tgttcgaagt tggtcactcc
     3001 gagcaagtca ctcaactcga aacccgagtc gtggaagctc agcgtctgat cgaaatcaga
     3061 ggcgaggaga aaatgtttcg caggtctctt gttcacaaac tactccgatc gactgcccct
     3121 cgtgccgcgc gactgttcgc gttggaaccc aagttaacag ccgcagtgcg acaaaaagga
     3181 gaaggtggtc acagccgaac gctgaaattt tctagcgcat cagtgaatta gcggtgtttg
     3241 cgccgcaatc gggcggtgta agggacgcat gccggattgt ggtgccgccc ggtaaaacac
     3301 gaggtcaatg agatgaaggg cccatctccg gcctgtgcca atcggcccaa catgccgcgg
     3361 atgtcaaaag cactttcagc cggcctgcaa gcgatgctgt acgtggcagg catttcgatg
     3421 gggatcgcgc acgcgcaaac cctcgaatcg ccgccttccg ctccttcgga gctgcagaca
     3481 cagatcgagg cgctggtcga atccggtccc ttcgagattc acggcgcgca catcgcctat
     3541 ccaaacgtca tccacgcgtt ctattcgcag cgcacgtttc gtcccgcctg ggccgacgcg
     3601 aaaaccgcga gcgaactgcg gcgcgcattg aaagacagtg aagcagatgg gctcgatccg
     3661 aacgactacc acctgccggt tctcgaacag ttgtcaggac cctccgcaac cggcgcaccc
     3721 ggcaatagtt cgacgcaggc tgccctcgag attctctaca ccgacgcgct gctgcgcatc
     3781 gccgaccatc tcatgttcgg aaaagtggac gcggcgtcgt tcgacgcgca ctggaactac
     3841 acgcgcacgc ccacgggcat cgacgtgccg cagagaatcg agcgcgcgct cgcgagtggc
     3901 gacctctatg ccgagatcga aaaactgaag ccgacgcacc gcatgtaccg gacgttgaag
     3961 caggaactgg ctcgctaccg gcaggctgcg gtggaaccgc gtcgcatcgt catcgccgcg
     4021 gggaaaaccc tcgagccgga cactcatgac gagcgcgtgc tggcgttacg cacgcgcctc
     4081 gcgatcagcg gcgatatcga tgccccccag gattccgcgt ccgatctcta cgacgccacg
     4141 ctgctcgccg cggttcgccg gtaccaggaa cgcatgggga ttctcgccga tgggaagata
     4201 ggcgcgcgca cgatcacgga gctcaacgtc ccgatcgagg aacgcattac ccagctcctc
     4261 gtcaatctcg atcgcgggcg cgtattgctg cacgacctgc cgaacgagtt cgtggtggtg
     4321 aacatcgccg cctactgggt ctatctggtg cgcggccagg aaatcatctg gagcgcgcgc
     4381 acccaggtcg gcaagaccta tcgccgcacg ccgatgttcc gctccgccat cgactacgtg
     4441 gtgttcaatc ccacctggac cgtgccgccc ggcatcatcc gcaacgacat tcttcccgaa
     4501 gcacgccgcg atccggcctc gatcacgcgc cgcggtctca acgtgctgga tgcacaaggc
     4561 aacaaggtcg atcccgcgag cgtcgactgg tcgagattca agagcggcaa cattccttac
     4621 acgctgcgcc aggatccggg accgaagaac gcgctcggcc gcgtcaaggt catgttcccc
     4681 aattcgtatt cggtgtatct gcacgacacg ccgtcgcaat cgaagttcga agagaccgat
     4741 cgcgctttca gctccggatg cgtgcgcacc gaacgtgcac tcgagctcgc ggagttgctg
     4801 ctggccaatc ctgcgtcgtg gaatccggcg accgtccagc ggacgatcga cgccggcacc
     4861 acgcagaacg tgacgctccc aaacaaggtg ccggtgttgc tcgcgtactg gaccgcctgg
     4921 gtggatccgc agggccgcgt taatttccgc cgcgacatct acggtcagga cgcgcagtgg
     4981 gccgcggcgc tcacgcgccc gtactcgccg cgaaaaatac cccagcttcc ctagcggcat
     5041 acgccgcgct cgagaatcgt gccgtggccc accggccggc cgacgctgag ccacaggctc
     5101 caggagtccg tggaggtggt gcccactccc acggtcaacg gaccgaatag cgtccgtccg
     5161 gtgaggaaca ccgaacctcc gtacaactca ccgtcttccc cgccatccag tcgatcgaag
     5221 acttcgccgc cggtcaggcc aaaccccatg tacagcgaca gattccggat cgggagcaga
     5281 tccttcactc gccagagata actcgtgctc gcgagccagt attcgcggac tcgcagttca
     5341 ccctgttcga aaccgggaaa actgtcgggc ccgcccacgg agaacatgcg atcgcccggc
     5401 agatcgctgt cgaaatccga accggcggcg accgtcatcc acaacacatc gcggcgaagc
     5461 ggcaaggcca tgcccaatcc gatctccgcc ttctcccaat cgcggtcggc tcccagcgag
     5521 ctgtcacttt tcatgtaatc gacagccgcg gcaaatccac gcgtgggatt gaacgccgta
     5581 tcacggctgt ccagttcggc gagcacgtgc atgccggcat ccacggggtc gccttcaggc
     5641 atgagcgacg agccaatatc cacttcgatt tcgcgttcgt cgtacaggta gccgattcga
     5701 gcctggccgt aacggcccac gtttgcgccg aagttgaact cgcccacgaa atcccggaat
     5761 tcgtagcgcg cgacccgctc gtccatggaa aacacatctt cgagcgagcg cgtgatcgcg
     5821 acgcgtggct cgatgaagaa ccggtgcgcg gaatcgagcg gctgatacag gccggtcgtc
     5881 agcagcgact cgccgccgag ctgcagttcg ttgaaccact gccctcccag tgaattcagc
     5941 caggtgcgga cgtgccggcc atacagcgcg aatgtcacat cgccttcgcg ggaagcgtat
     6001 gcgccgaggt ccacgcgcag gtagttggga ccgaatttct tttcctgcgg cagccaggtg
     6061 agtgtcgggg agtcgcgctc tccatcgagg cgataactga cggagtcgaa atcctgcagt
     6121 gcggagagcc gctgcgcttc ttcgctgatc gtgacgatgt cgacgggatc gcccggctgc
     6181 actttctgcc ggtgagccag gtagtccgga ttgacgcgct cgagtccttc gtagcgcacg
     6241 cccgcgagct tgccttctat cttctgcgat tgagtgacgc tggtccgcca cgcgacgtac
     6301 tgtttctccg gcaccgagaa ctggctgagc gcgccggcca tcttgcgggc cgcgtccacg
     6361 ccgagcggca cggtctccgg cacacgctcg aaatcggcgg tggtgatgtc gcccatgatc
     6421 acgtcgatgc gcacatcgcc gggtcgcagt gacgccagct gcaactcttc gttcgcgatg
     6481 atcatcacgt ccatcgcacg ccccagcagt tgggtggccg actgcagttt tttcggattc
     6541 gcctcgggct cgaccaggtt cacgacgatg acctggtccg cgcaaaggtt tcgcgcgatg
     6601 tcgatcggaa tgttccgtac gagaccgccg tcggagagaa tcatgtcgtc cagcattacg
     6661 ggcgcgaacc cacccgggat cgccatgctg gcgcgcatgg ccgtcgccag gtcaccttgg
     6721 tcgagtacga ccatcttgct ggagaccatg tccgtcgcga tcgcgcgata gggaatcggc
     6781 agtttgttga aatcggtttc caggcgcgcc cgcgccacat acacgcgcag caggtcctcg
     6841 acattgctgg tgttgatgag ccctgccggc gccgcgacgc catccggcgt aatgccgaac
     6901 tcgaactcgt tgctgtaggt ggggcccgag cgtttttgtt cgatgggctc gaggtcgcga
     6961 cggccctggc tgccgaccac gcgcttccag tcgatgctga tcaggaactt ctcgagatcg
     7021 tcggcgggaa tgccggcggc ataaccgccg ccgatgagcg cgcccatgct ggtgccggcg
     7081 atgcagtcga tgggcacgtg cagttcttcg agcaccttga gcacgccgac gtgcgcgccg
     7141 cccttggcgc cgccgcccgc gagcacgaga ccgatgcgag gtctgcccga cgcaatcgtc
     7201 gcgtcggcac ccagggctgc gagcggactc aatacctgca acgcgcctgc caatagagcg
     7261 atgcaccgta gccggcggga agattgcatg cgaacacctc ggttgtgcgg cttcagcttc
     7321 cactgatacc gccgaggtga ctatcgacaa ttcgcaggtc gcggagtctc tagaactcct
     7381 tccactccat gtcggcgtcg ctattggcga cgcgcgcgga cgcacgggcc ttgggctccg
     7441 ccgcaggcgc cgcgaccttg ctgttccacg gcctgctcga cttgcggcga tcggccggcg
     7501 tctgtgaagg cgctacctcg ggcgcagcgg cgctggctcc cgtggcgttc acctggtaac
     7561 gcgtcatcga tgaattcaat tctcgcgcct ggtcggccat ggcctggctc gcggccgacg
     7621 cctgctcgac cagcgccgcg ttctgctgcg tgagttcgtc gagctgcatc acggccttgt
     7681 tcacctggtc gatgcccgcc gactgttcat gcgacgcagc ggcgatctcg gacacgatgt
     7741 cggtgacctt cttcaccgcg gcgacgatct gttcgagcgt cgcacccgac tgcgtcacca
     7801 gcttcgaacc ttcgtcgacc ttggtcacgg tgtcctggat gagtcccttg atttccttgg
     7861 cggctgccgc cgaacgtccg gcgaggttgc gcacttcggt ggcgaccacg gcgaacccgc
     7921 ggccctgctc accggcgcga gcggcttcca ccgcggcgtt gagcgccagc aggttggtct
     7981 ggaacgcgat cccgtcgatc acgctgatga tgtccgcgat cttcttcgag gcgccgttga
     8041 tgtcgaccat cgcgtcgacg gccttcgaga ccaccgcgcc acccttctcc gcctgatcgc
     8101 gcgcggcgac ggcgagcttg ctcgcatgcg ccgcgttgtc cgcgttctgc ttgacggtgc
     8161 tggtcatctc ttccatcgac gatgccgttt cttcgagcga cgaagcctgc tcttcggtgc
     8221 gctgcgagag gttggcgttg ccctggctga tttcgtccgc gccgcgactc acttcggccg
     8281 aagccgtctt cacctgcgac accagcgccg acagcgtgct ggtgagatcg ttgatccccg
     8341 agccgatctg cacgagcagt ccggccttgt ccttgatgtc gatgcgccgc gtcaggtcgc
     8401 cctggttggc cgcggccacg acgccgcgca cttcggcgac cacggtcgcg atcgcgtcca
     8461 cgagttcgtt gatgccaccg gacaacgtga ggaagaagtc gcgtttgcct tcgagcgaaa
     8521 tgcggttctc gagattgccg gccgtcaccg cgccgatgac ctgctgcaat tcggtttccg
     8581 tggcgacttc ctgcgtgcgg tcgaaccact gcagcaccgt gcccaggcgg cggccggtgt
     8641 cgtctttcat cgggctcatg atggtcttca tcgagcggcc accgatcact tcctggcgcg
     8701 tcacgatatc cgtcaggcgt tcgatcacgc cacgcagagc cgcggggtcg cgcgtcatcg
     8761 attccacgct ggcgcccagc aacttgccgg cgtcgaagcc cggcagttcc ttgcggaacg
     8821 attcctgcgc gcgcgtcagc atctgcgcgg cggcggggtt cacgaagatg atcttcatgt
     8881 cgttgtcggc gcacatcacg ttggcgtcga ggcgatcgag cgcgccacgg aacgccgcgt
     8941 tcaacagcgc ttcctggcgc tgcttcgtga cgtcattcag ataggcgacg accttgaacg
     9001 gtttgccgga agcgtcgacg atcggattga acacgccctg aaaccagatc tcgcgtttgt
     9061 cgcgcgagac cacgcggtat tcgccctggc ggctttcgcc acgcttcagc gccggccaca
     9121 gttccttcgc gtcgtcggcg aacatcgagt agttcttgcc ctgcacttct tcgagcgagt
     9181 agccgagaag cttcaggaag atgtcgttgg cgttgctgac gtcgccttcg agcgagcatt
     9241 cgatgacgcc ctgcaccttg gcgatcgcgt tgatctgtcc ctgatagtcg agcttggaat
     9301 gcagcaggct gtccttcatc tggcgcaacg cgcccatgag gctgatcgcg tcgtggcggc
     9361 cgggcagcat ttcgaattcg agttcgccgg acgcgacgcg cctggctacc tgcgcggcga
     9421 cggcgggatc cgcgccgagc ttgcgcatgg cgctgcggat gatgaaccac gccatcaaga
     9481 tggccagtaa cgcgccggcg ccgagcagca ctttgccgtt gcgcagcgcc agatcgtaat
     9541 tcttgttggc gttgtccagg tccagattgg cagcggtgag ctgcatggcg atcagcttct
     9601 tcagattgct cgcgccgtcg gccaacgcag gccgcagcgt ttcattgcgc caggccgcga
     9661 ggtccgcgat gtcgttggtc tcgagtttct tgagcagctt gtcgatcttg ccgtaggcgc
     9721 cgtcgagcat cggcgtggtc tcgtcggcga gctcctgctc ctccgtgatc atgtcgccga
     9781 ggaaatagtt gtcccagtcg tcgcgcgcct tgtcgatggc cttcttgatc tgatcgtggg
     9841 cgtcgatcgg ggtcaccttc ttttcgatga cgtcgagcga cgcctcgagc gacagcgaca
     9901 atgcgtcggc gacgccctgg atcgactgtc ccgacaccat gcggttgtcg atggtgtgtt
     9961 tcagcgttcc ctgcgtgctc ttcacctgct gcatcgcata gaagatgagt cccgtcgcga
    10021 tcgccagggt gatggcaacc agcgcgtaga gctgcaacga gaggcggaaa tttttgaaga
    10081 cgttgagtcg catacgaagt cccggtgaag aaactgtcag gccgccgccg cgctggcgag
    10141 gccgatggaa gtctgctcgc tcgaagcgag actctcggcg tggagcagga tcagcatccg
    10201 ctcgtcgtga gtcgcgatac cttcgatgaa ttcgctcgag tccgcgccac tcatggcggg
    10261 cgccggcttg atgctcgcgg cgtcgatgtc gaccacgtca ttgacgttgt cgacgacgat
    10321 gccgcattcg cgctggccgt tcgccgtgcg caacgacaac acgatgatga cggtgagcgg
    10381 cgtgaatgcc gccgactcga gcgcgaagcg gacgcgaaga tcgatgaccg gcacgatgag
    10441 gccgcgcagg ttcagcacgc cgagcatgga agcttcggac tgcggcatgc gcgtgaccgg
    10501 cgaccagccg cgaatctctt tcacccgcag gatgtccacg ccgtagacct ctttgccgag
    10561 cgaaaaggtc agcacctgcc gggtagtggt tccccctgct ccagccgctt ctcgtgagtc
    10621 tttgttctgc atcgatgcga tatcggcgcg gcgacacctt aatggaggac gcacatcaca
    10681 tttttcgggc cgaccgcctg tgatgccgct ctaaaggatc gcgggattga tgccgatagt
    10741 ggtggagaga tcgtgagagc gtcgcccagc cggggacgca tgactggctt ttgttaactt
    10801 ggagagaaga atgaaggtgc tagtttcgat gacggttctg tgggctgccc tggccgttcc
    10861 ggccctggcg tgcgagcggc ccagcgcccc gacgtcgatc cccgacggcg cgtccgcttc
    10921 caaggaagac atgttggctg ccaagaaagc ggtcgacgcg ttcaagagcg gcatggaaga
    10981 gtacatagcg tgcgagaaga gcggcgcgaa gaaagacggc gccgcggctg aactcgtgaa
    11041 ggtcgccgac cgcttcaacg cgcaggtcaa ggcgttcaag gcgaagggct gaagcccagc
    11101 ccctatctac cgcgggcggg aaccgtgcgg ggtcccgctc gcaggtattg gcgttgtcat
    11161 agcgccttgt agctgaactt ctgtccaccg accgcggcct ggtacatcag cccgcccttg
    11221 gccacggtga acaccgccat tcccttgtgc cagttgccgg tcgttgccgc gtccttcttg
    11281 ccgccggccg cacccgcagc cgcgccggct gttccggcac tggcggtcgc cgacgctgta
    11341 atcgcgacgg cctggaccgt ggcgtcgaat tcgaaggtac cgctcgcgaa ttcgtcgaag
    11401 gcccgcttgt cctggaagaa gatgatctgg ctgaacgcct gaccaccagc ctgcaggccg
    11461 atgctgagtt gattcatctt cacgtcgccg acgtgtttgc cgtgtatgta ggcgcgaccg
    11521 gaaccatgtg cggcgccgac gcccaacccg cccttgccga cgttggggaa gaccgcgtat
    11581 ccatagctgg ggccaaagaa cgcgctgctt tcgccggcgt tcatgaagat ctggacggtg
    11641 tcgtcgaagg catcggcgga ggcggttaca ccggccagca acatgaccgc cgcgaacatc
    11701 agagttgaga gcttggactt catggttcct cccccgagat tgcccggatg atggaacatc
    11761 ggaggcgaag tcgtcgtccg cccttggtcc gattgccgat tgagattggt gccggctgca
    11821 gggatcgaac ccgcgacccc ctgattacaa atcaggtgct ctaccaactg agctaagccg
    11881 gccttttggc tgggcggatt ttaggcgtgt gcgccgcaac gcgctattgc gcggcttccg
    11941 tttcttctgc cggcggacag gcccggaatt cgagctccgt gtcgccggcc gggaatttct
    12001 ggccttcgag cggcggcgaa tccgatttga gcgtgagatc gagccagatg gcggtcccgg
    12061 acctttcccg ggcctcgatc tgcggcagca gctgcaaccg ggcgacgcgc tcggactggg
    12121 ccacggcccg gccttcgtcg gagaacacgc ccagcgagat gcggcgcgaa cccgcatcgc
    12181 cgacggcctg cgcatcggtg atgccgccct tcttcaggcg gtcgagcagg cgccgttcgc
    12241 cggtttcggt cggcgggctt tcgaggtaga cccaataccc ttccagatcg cgggtctcga
    12301 cttcacgggg cagcgacgtg tagccgctgt cggccagagt cgaccgtgcg cgtgcggcga
    12361 gttcattggt ggcgaacggg ccgaccgtga cgcaaccgcc gtcgcccaca gccgcgggac
    12421 gtgactcgcg cacgagctca agcggcggcg cgttgacctt gggcgaaacg ggcatttgcg
    12481 cttcgccggg cgcaagccac tgcatccacg cgaagaacgc gagattcgct aataagagaa
    12541 ggagtaaggc tgcacgcacg cgaggcgcga gtgtatcaaa tgcgttatgt agcgttcgcg
    12601 aacaccgcga ggccccgaag cacgaggtcg gggaccatgc gcaacgggct ggtgaggtag
    12661 cgcttcaact cgggtgcggc accaccggta agaacgagga tcggccggcc gccaaggttt
    12721 cgttgcgctt cgagcagggc gcgatcgatg
//
LOCUS       DATDEU010000475         7007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1403070, whole genome shotgun sequence.
ACCESSION   DATDEU010000475 DATDEU010000000
VERSION     DATDEU010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403070"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2304)
                     /locus_tag="VIV63_11340"
     CDS             complement(<1..2304)
                     /locus_tag="VIV63_11340"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6125235.1"
                     /translation="MRPLGVMCVLLLLGGCDRFTSPERRVDRADAAMAAGNYGAAVVD
                     LKNALKEKPAFDRAHLLLADAALWLGDARGAEQELGKIKGTVDEARLADVEIRLALAQ
                     GKADEALQRLTAEPQPLPAARRDLLKGHALMRLARSREAQQAYEAAAAADGKLIEARA
                     GALEARIAQGDRAAGLAGLRELTQSSPESAEAWLTYGLTLAGNTDFRATIDALNQARK
                     LSARQLPVSRQVSLLVALAEAQLQAGDIKGAADAASGLSRVAPDTPVSLYIASRISMA
                     NNDYSGAVAKLRKIKQTAPGFFEARMLLGMALLAEGNAQQAAVELNEVLVQNPAHAGA
                     RQLLAQVRLQLDNPDGALRMLGPALANQPDDTQVNALIEAARSRLGAQQSVALLEEML
                     EQDPQNRGLASQLANAYLQAGEPDKAVALLRKGGENADDVRRAAALVSGIAASEGTSA
                     ARREIEAMLAANPQSPYLANLAAALYARTGDYAAARRALNDALARGADPASLLLTLGQ
                     LEWTAGDRKAAGAAIARLLEMQPDNAAAHMAAGEIAMVGGDADAARKHFEAVRAARPN
                     SIDARMRLAQLALRSGAMKTADDLLAEAIKLAPQRAEVRNAAGVLNLNSGRADQAITH
                     LRAALDIDPKNAVIWFNLARAQHTLGQKLAERASLMQAAEIQPDWLPVQSALAIREIE
                     SGDAAAAMARAATYRAAHPRDVAALAFEGDIAFAARRFAAALDAYRAAYNLKPAGVLA
                     EKFYRAAVSGNTGNATELLVRWSNANPG"
     gene            complement(2319..3797)
                     /gene="xrt"
                     /locus_tag="VIV63_11345"
     CDS             complement(2319..3797)
                     /gene="xrt"
                     /locus_tag="VIV63_11345"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HEY6125236.1"
                     /translation="MSPAAAIPGRLARPVFAILLVLGTFLALWPTTQSLALRWTDTVN
                     RAYTHGALVVVLCCFLLWHQRDALARVVLRPSWVACLSGLALGIAWLILLRAGVQIGH
                     QALLPLIAFCAVWAAFGSPAMRVLWLPVLYAYFTVPVWDLLNPLLQWLSTFAVRGFLS
                     LIGLPVYFDGLHFQIPAGIFEIAGGCSGLHFFIVGVAIAVFYGELHRDRLGTRMKLVA
                     LAIGMAMLTNWVRIAIIIVAGHLTDMQHYLVSGEHYTFGWAMFAVSMAIYFLIVRRWT
                     AESVAAAPPSLPGASLAPLGVVLACVTLAMPGLWQWLDTNQASPASAAAEKLPATVVG
                     WRELPPRNEPGPAEFVNADGFEARTFNGDGEPVDAYRAFYFSQEQGRELAGYANRPQG
                     PDMQVRSNVVTPDGLWREIKAQYSADDDWLVWFAYRVDGHRDVDALRAQLRYGVNSLR
                     GNPLSSAWVLRTRCVADCPMARARLARFARDSGMDVSGKEVR"
     gene            complement(3794..5149)
                     /gene="prsR"
                     /locus_tag="VIV63_11350"
     CDS             complement(3794..5149)
                     /gene="prsR"
                     /locus_tag="VIV63_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEY6125237.1"
                     /translation="MAEDKLKLLIIEDDEGLQKQLKWCFDEYEVLMASSRAEALALLR
                     RHEPHVVLQDLGLPPDPEGVSEGMATLSLILSVDSTTKVIVVTGNNDRDNAVRAVSLG
                     AWDFYQKPVDTDVLRLLVSRAFHMYALERQNRMLLETQATSPLEGFVATDDAMLKVCR
                     RIEKVAPTNATVLILGESGTGKELVARAVHAQSGRSGSRFVAINCAAIPEQLLESELF
                     GYEKGAFTGAGKTTPGKIEVASGGTLLLDEIGDMPLPLQAKLLRFLQDHVVERVGGRQ
                     EIPVDVRVVCATNKDLQALITENRFRQDLYFRIGEVTVEVPPLRERKGGPTALAHTML
                     RKFAGLQGKPRRGFTQDAIAALEAYAWPGNVRELENRVKTAMIMGESSLLTAEDLNLK
                     ESGDGKLLFNLKEVRTRAERQAIRQALAITQGNISHTADLLGVSRPTLYDLMEKYGIV
                     N"
     gene            complement(5131..>7007)
                     /gene="prsK"
                     /locus_tag="VIV63_11355"
     CDS             complement(5131..>7007)
                     /gene="prsK"
                     /locus_tag="VIV63_11355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HEY6125238.1"
                     /translation="EGASWVVFAADGLRIAAWFIALEALSTAEFPRWLRRTVLAACTA
                     MVASAVVGWASGYEPAAVSPALLAVLGLVAALGTLFASLRVWRHAIPAAKRAIRWCTA
                     GAAAQFVFDALAYLPIGDGGGEPDWVLPRITFAVLSVWPLMTAAWQLPVAAPRLFISR
                     QFAFYASTLIVLCLYAGVTVVGVIYARANAGEWSAPLQALFIGTAVVLLIAFLLAEWP
                     LRRLRVFIATHFYRNKYDYRLEWLRFVQTLSIGETEDARRNAIRAVAQIFGSPRGLLV
                     LRDPAGGQFYLQAAWPEERLAFPECTPVAVDDSLPRFLHDRQWVVDLHEYGADPRLYG
                     DLALPAWLSPGSPWRLIVPLLVSDQMLGFLVLAAPPDPFDVNYEDRDLLKTVGRNVAV
                     HLAQRRADEQLAESRQFDAYNRFAAFVMHDLKNSVAQLQLLVANAQRHRHNPVFFDDA
                     IGTIRNTSERMTRLIEQLQRRETQGVSRAVDLAAAIQAAVARAEARLPATTVTGDITG
                     VQVTADPDRLAAVIEHVIRNAQEATPSTGTVGIELQMLDSEVRVTVVDNGAGMDEDFL
                     RHRLFRPFDSTKGAKGMGIGAYQAREYARDLGGDVEVWSAPGRGTRFCIRLPAWLKTN
                     "
BASE COUNT         1174 a         2396 c         2327 g         1110 t
ORIGIN      
        1 gccgggattc gcgttcgacc agcgcaccag cagttcggtc gcgttgccgg tattgccaga
       61 gaccgccgcg cgatagaact tttccgcgag caccccggcg ggtttcaggt tgtaggccgc
      121 gcggtacgcg tcgagcgccg cggcaaaacg acgcgcggcg aaggcgatgt cgccttcgaa
      181 cgcgagcgcc gccacgtcac gcggatgagc ggcgcgatac gtcgcggctc gcgccatcgc
      241 tgcggcggca tcccccgatt cgatctcgcg gatcgcgagc gcgctttgca ccggcagcca
      301 gtccggctga atttccgcgg cctgcatgag cgacgcgcgt tcggcgagct tctggccgag
      361 cgtgtgctgt gcgcgcgcca ggttgaacca gatgacggcg ttcttcggat cgatgtcgag
      421 ggcggctcgc agatgcgtga tcgcctggtc ggcgcgcccg gagttcagat tgagcacgcc
      481 ggcggcgttg cgcacctcgg cgcgctgcgg cgcgagctta atggcttcgg cgagcagatc
      541 atcggccgtt ttcatggcac cgctgcgcag ggcgagctgc gcgagacgca tgcgcgcgtc
      601 gatggagttc gggcgcgcgg cgcgcaccgc ctcgaagtgt ttgcgcgcgg cgtccgcgtc
      661 gccaccgacc atggcgattt caccggcggc catgtgcgcg gccgcgttgt ccggctgcat
      721 ttcgagcagc cgcgcgatcg ccgcacccgc ggccttgcga tcgcccgcag tccattcgag
      781 ctgaccgagc gtgagcagca gcgacgcggg atcggcgcca cgagccagcg cgtcattgag
      841 tgcgcggcgc gcggcggcgt agtcgccggt gcgcgcgtag agcgccgccg ccagattcgc
      901 gagatacggg ctctgcgggt tcgcggcgag catcgcttcg atctcgcgac gcgcggcgga
      961 cgtgccttcg ctcgcggcga tgccgctcac cagcgcggcg gcgcgcctca cgtcgtcggc
     1021 gttttcgccg cccttgcgca gcagcgccac tgccttgtcg ggctcgcccg cctgtagata
     1081 ggcgttggcg agctgcgagg cgagcccgcg gttctgcgga tcctgctcga gcatttcttc
     1141 gagcagcgcc accgattgtt gcgcaccgag gcgcgagcgc gccgcttcga tgagcgcgtt
     1201 gacttgggtg tcatcgggct ggttcgcgag cgccggcccg agcatgcgca atgcgccatc
     1261 gggattgtcg agctgcagcc gcacctgcgc gagcaattgg cgggcgccgg catgcgccgg
     1321 gttctgcacc aggacttcgt tgagttcgac ggccgcctgc tgggcgttgc cttcggcgag
     1381 cagcgccatg cctaacaaca tgcgcgcttc gaagaaaccc ggcgcggtct gcttgatctt
     1441 gcgcagcttg gcaaccgcgc ccgagtaatc gttgttggcc atcgatattc ggctggcgat
     1501 gtatagcgac accggcgtat ccggcgcgac gcgcgagagg ccggacgcgg catccgcggc
     1561 gcccttgatg tcaccggcct gcaactgggc ctcggccagc gcgacgagca ggctgacctg
     1621 ccgtgacacg ggaagctggc gcgcggatag cttgcgcgcc tgattcagcg cgtcgatggt
     1681 cgccctgaag tcggtgttgc cggccaacgt cagaccgtag gttagccagg cctccgccga
     1741 ttccggcgac gattgcgtga gctcgcgcag gcccgcaagt ccggcagccc gatcaccctg
     1801 cgcgatgcgc gcttccagcg cgccggcgcg cgcttcgatg agtttgccgt cggccgcggc
     1861 cgcggcttcg tacgcctgct gcgcttcgcg cgatcgcgcg aggcgcatga gcgcgtgacc
     1921 cttcaacagg tcgcgcctgg ccgcgggcaa cggttgcggc tcggcggtga gccgttgcaa
     1981 cgcctcgtcg gccttgccct gcgcaagcgc caggcgaatc tccacgtccg cgagccgcgc
     2041 ctcgtcgacc gttcccttga tcttgcccag ctcctgctcc gctccgcggg catcgcccag
     2101 ccacagcgcg gcatcggcga gcagcaggtg cgcgcggtcg aacgcgggtt tctccttgag
     2161 tgcgttcttg aggtcgacca cggccgcgcc gtagttgccg gccgccatgg ccgcatccgc
     2221 gcggtccacg cgcctttcgg ggctcgtgaa gcggtcgcag ccgcctaaca acaacagcac
     2281 gcacatcacg cccaatggcc gcatcgcccc tctcctcgtc atcgcacttc ctttccagaa
     2341 acgtccattc ccgaatcacg cgcaaagcgc gcgagccgcg cgcgagccat cggacagtcc
     2401 gccacgcagc gcgtgcgcag gacccacgcc gacgacaagg gattgccgcg gagcgagttc
     2461 acaccgtaac gcagttgtgc ccgcagcgcg tcgacatcgc ggtggccgtc cacgcgatat
     2521 gcgaaccaca ccagccaatc gtcgtcggca gagtactgcg ccttgatttc acgccagagt
     2581 ccgtccggcg tgacgacatt ggagcgcacc tgcatgtccg ggccctgcgg ccgattggcg
     2641 tacccggcga gttcgcggcc ctgctcctgc gaaaagtaga acgcgcggta ggcgtcgacg
     2701 ggttcgccgt cgccgttgaa cgtgcgcgct tcgaagccat cggcgttgac gaactccgcc
     2761 ggcccgggtt cgttgcgtgg cggaagctcg cgccagccca cgaccgttgc cggcagcttt
     2821 tccgcggccg cgctcgcggg cgacgcctgg ttcgtgtcca gccactgcca caacccgggc
     2881 atcgccagcg tgacgcaggc gagcacgacg cccagcggcg caagcgacgc acccggcagc
     2941 gatggcggcg ccgccgccac ggactcggcg gtccagcggc gcacgatcag gaagtagata
     3001 gccatggaca ccgcgaacat cgcccagccg aacgtgtagt gctcgccgct cacgagatag
     3061 tgctgcatgt cggtcaggtg gccggcgacg atgatgatgg cgatgcgcac ccagttggtc
     3121 agcatggcca tgccgatggc gagcgccacg agtttcatgc gcgtgccgag ccggtcgcga
     3181 tgcaactcgc cgtagaacac cgcgatggcg acgccgacga tgaagaaatg caggccactg
     3241 caaccgccgg cgatctcgaa gattcccgcg ggaatctgga agtggagacc gtcgaagtac
     3301 accgggagcc cgatgaggct gagaaatcca cgcaccgcga atgtcgacag ccattgcaac
     3361 agcggattca gaaggtccca gaccggcacg gtgaaatacg cgtacaggac cggcagccac
     3421 aacacgcgca ttgcgggcga tccgaacgcc gcccataccg cgcagaaggc gatcagcggc
     3481 aacagcgcct ggtggccgat ctgaacgccg gcgcgcaaca ggatcagcca ggcgattccg
     3541 agcgcgagcc ccgagagaca cgcgacccac gagggacgca acaccacgcg cgccagcgca
     3601 tcgcgctgat gccagaggag gaaacaacac agcaccacca ccagcgcacc atgcgtgtag
     3661 gcgcgattga cggtatcggt ccagcgcagc gcgagactct gcgtggtcgg ccacagggcc
     3721 aggaacgtgc ccagcaccag caggatggca aacaccggcc gtgcgagacg gcccggaatc
     3781 gcggcggccg gactcaattc acgatcccgt atttttccat gagatcgtag agcgtcgggc
     3841 ggctgacgcc gagcagatcc gcggtgtgcg agatgttgcc ctgggtgatg gccagcgcct
     3901 gacggatcgc ctggcgttcc gcgcgcgtgc gcacttcctt gagattgaag agcagtttgc
     3961 cgtcaccgct ctccttcaga ttcagatctt cggccgtcag cagcgaactc tcgcccatga
     4021 tcatcgccgt cttcacgcga ttctcgagct cacgcacgtt gccgggccac gcataagcct
     4081 cgagcgcggc gatggcgtcc tgcgtgaagc cgcgccgcgg cttgccctgg agacccgcga
     4141 acttgcgcag catggtgtgc gcgagcgcgg tggggccacc cttgcgttcg cgcagcggcg
     4201 ggacttcgac cgtcacttcg ccgatgcgga aatagagatc ctggcgaaaa cggttctcgg
     4261 tgatcagcgc ctgcagatcc ttgttggtgg cgcagacgac gcgcacgtcg accgggattt
     4321 cctggcgacc gccgactcgc tcgaccacgt ggtcctgcag gaaccgcagt agtttggcct
     4381 gcagcggcag cggcatgtcg ccgatctcgt cgagcagcag cgtgccgcca ctcgcgactt
     4441 cgatcttgcc gggcgtggtc ttgcccgcgc ccgtgaacgc gcctttctcg taaccgaaca
     4501 gctcgctttc cagcagctgc tccgggatcg ccgcgcagtt gatggccacg aagcgtgagc
     4561 cgctgcgacc actctgcgca tgcaccgcgc gcgccacgag ttccttgccg gtgccgcttt
     4621 cgccgaggat gagcaccgtg gcattggtcg gcgccacttt ctcgatgcgc cggcacacct
     4681 tgagcatggc gtcgtcggtc gcgacgaaac cttcgagcgg tgacgtcgcc tgcgtttcga
     4741 gcagcatgcg gttctgccgc tcgagcgcat acatgtggaa tgcgcggctc accagcaggc
     4801 gcagcacatc ggtgtcgacc ggcttctgat agaagtccca cgcaccgagt gacacggcgc
     4861 gcacggcgtt gtcgcgatcg ttgttgccgg tgaccacgat gaccttggtg gtgctgtcga
     4921 cgctcaggat caaagacagc gtggccatgc cttcgctgac tccttccgga tcgggtggca
     4981 gacccaggtc ctgcaaaacg acatgcggct cgtggcggcg cagaagggcc agcgcctcgg
     5041 cgcgcgatga cgccatcaac acttcgtatt cgtcaaagca ccacttgagc tgcttctgca
     5101 acccttcgtc gtcttcgatg atcagcagct ttagtttgtc ttcagccacg ccggcagcct
     5161 aatgcagaac cgcgtgcccc gtccgggagc actccacacc tcgacgtcgc cgccgagatc
     5221 acgcgcgtac tcgcgagcct ggtaagcacc aatacccatg cccttcgcac ccttggtgct
     5281 gtcgaacggc cggaacagcc ggtgccgcag gaaatcctcg tccatccccg caccgttgtc
     5341 gaccacggtc acgcgcacct cgctgtccag catctgtagt tcgatgccca cggtgcccgt
     5401 ggaaggcgtg gcctcctgcg cgttgcggat gacgtgttcg atgaccgccg cgaggcgatc
     5461 ggggtcggcc gtgacctgca cgccggtgat atcgccggtc accgtggtcg ccggcaatcg
     5521 cgcttcggcg cgagccacgg ccgcctggat cgccgccgca agatccaccg cgcggcttac
     5581 accctgcgtt tcgcggcgct gcaactgctc gatgagacgc gtcatgcgct cggaggtatt
     5641 gcggatggtg ccgatggcgt cgtcgaagaa caccgggttg tgccggtggc gctgcgcatt
     5701 ggcgactaac aactgcagct gcgccacgga attcttgaga tcgtgcatga cgaacgccgc
     5761 gaaccggttg taagcgtcga actggcggct ctcggcgagc tgctcgtcgg cgcggcgctg
     5821 ggcgaggtgc acggcgacgt tgcggcccac ggtcttgagc aggtcgcgat cctcatagtt
     5881 gacgtcgaac gggtccggcg gcgcggccag caccaggaac cccagcatct ggtcgctcac
     5941 cagcagcggc acgatcagcc gccatgggct tcccgggctc agccatgcgg gcaacgcgag
     6001 gtcgccataa agccgcgggt ccgccccata ttcatgaagg tccaccaccc actgccgatc
     6061 gtgcaggaaa cgcggcagcg agtcgtccac cgccaccggc gtgcactccg gaaacgccag
     6121 gcgttcttcg ggccaggccg cctgtagata gaactggcct ccggccggat cgcgcagcac
     6181 cagcagcccg cgcgggctgc cgaagatctg cgcgaccgcc cggatggcgt tgcggcgcgc
     6241 gtcttcggtc tcgccgatgg ataacgtctg cacgaaacgc agccactcga ggcggtagtc
     6301 gtacttgttg cgatagaaat gagtggcgat gaacacgcgc aaccgccgca gcggccattc
     6361 cgccagcagg aatgcgatca gcaggacgac cgcggtgccg atgaacagcg cctgcagcgg
     6421 cgcgctccac tcccccgcat tggcgcgcgc atagatgacg ccgaccacgg tgacgccggc
     6481 atacaggcac aagacgataa gagtggatgc gtagaacgcg aattgccgcg agatgaacag
     6541 gcgcggagcc gcgaccggca attgccaggc cgcggtcatc aacggccaga ccgacaacac
     6601 cgcgaaggtt attcgtggca ggacccagtc gggctcgccc ccgccatcgc cgatcggcag
     6661 gtaggcgagc gcatcgaaga cgaactgcgc ggccgcgccg gccgtgcacc agcggatggc
     6721 gcgtttcgcc gcgggaatgg cgtgacgcca cacgcgcaag ctggcgaaca gcgtgccgag
     6781 cgcggcgacg aggccgagca cggctaacaa ggctggactg acggccgcag gctcgtaacc
     6841 cgatgcccag ccgaccaccg ccgacgccac catcgcggta caggccgcca gcacggtgcg
     6901 gcgaagccat cgggggaact cggcggtaga tagggcctcg agcgcgatga accaggccgc
     6961 gattcgcagg ccgtcggccg cgaacaccac ccagctcgcg ccttcct
//
LOCUS       DATDEU010000476         4501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_89472, whole genome shotgun sequence.
ACCESSION   DATDEU010000476 DATDEU010000000
VERSION     DATDEU010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89472"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..542
                     /gene="pal"
                     /locus_tag="VIV63_11360"
     CDS             27..542
                     /gene="pal"
                     /locus_tag="VIV63_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY6125239.1"
                     /translation="MKKNLVSLSVLLATLTLFAGCKTKPPPVVETPPPQTSDTTGVED
                     PNASIGDDAQMGPTGELLSKRIVYFDFDRAEIRADSQSVVAAHAAYLAKNPNQKVRLE
                     GHADERGSREYNIGLGERRGQTVRRALALQGVAETQLSTVSYGEERPAAAGSDEQAYA
                     ANRRVEIVYLK"
     gene            559..1296
                     /gene="ybgF"
                     /locus_tag="VIV63_11365"
     CDS             559..1296
                     /gene="ybgF"
                     /locus_tag="VIV63_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY6125240.1"
                     /translation="MNTSRLLGLAGALALVGCAVSPEEDPVMIRMNDLDARVQRIERV
                     LTNQSLLELAQRLDALQGEVRNLRGEVELLQNETQGGKTQSRNLYGDLEKRLAALETL
                     GGVGSAPAPGVVPVPVSGASEQATYDAAMAALRGGNYDKAVATFREVVTNYPAGDLAP
                     NAQFWIGESYYTKGDLESAVTAYRKVLADWPNSRKAPDAMVKLGFSLSDLRRTPEART
                     TLEEVVRRYPGTNAAQLAADRLKRLGR"
     gene            1307..1984
                     /gene="queE"
                     /locus_tag="VIV63_11370"
     CDS             1307..1984
                     /gene="queE"
                     /locus_tag="VIV63_11370"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016711990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEY6125241.1"
                     /translation="MNAAASELPPGAERRLKITEIFYSLQGEADTVGYPTVFVRLTGC
                     PLRCHFCDTAYAFHGGEWRSVGEVLETVAGFSPRYVCVTGGEPLAQKNCLPLLTQLCD
                     AGYRVSIETSGAMPLDGVDERVIRVVDVKTPGSGEERRNRYDDLNLLRPEEQIKFVIC
                     DRADYEWSRDKLSSLNLAARCQVLFSPSADQLPARELADWILADRLPVRFQMQLHKVL
                     WGNVPGK"
     gene            1987..2691
                     /gene="queC"
                     /locus_tag="VIV63_11375"
     CDS             1987..2691
                     /gene="queC"
                     /locus_tag="VIV63_11375"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005399706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY6125242.1"
                     /translation="MTKRAVVLLSGGLDSATVLALARAAGLDCYALSVSYGQRHQAEI
                     SAAARVAHALGAREHRIMHVNLGRIGGSALTDNSIDVPVTPGEGIPVTYVPARNTIML
                     SLALAWAEVLKATEIHIGVNAVDYSGYPDCRPEFVAAFQQLATLATKAGVEGANLLIR
                     APLIDFSKAQIVREGARLGVDYSLTVSCYQADDQGLACGRCDSCRLRREGFVSAGIPD
                     PTRYLSFQGQGDEKIV"
     gene            2710..2785
                     /locus_tag="VIV63_11380"
     tRNA            2710..2785
                     /locus_tag="VIV63_11380"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2743..2745,aa:Lys,seq:ttt)
     gene            2807..4441
                     /locus_tag="VIV63_11385"
     CDS             2807..4441
                     /locus_tag="VIV63_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6125243.1"
                     /translation="MSDPEFDYVIIGAGTAGCLLANRLSADPSRRVLLVEAGPPDNYA
                     WIHIPVGYLRCIGNPRTDWRYRTEPEAALHGRDLLYPRGKTLGGSSSINGMIYMRGQA
                     RDYDAWARATGDDAWSWPNSLPDFRAHECSFRCEPGREDPMHGSGGEWRVSRQRLRWD
                     ILDSFAEAAEQAGFPRIPDFNTGDNAGVSYFDVNQRGGFRVSSAKAFLKPARARRNLT
                     VWTGALTERLHIERDGNGALRCSGATLVHNGQHIRVRATRELLLSAGAIATPQLLQLS
                     GLGPAPLLREHGIEVLLDSPGVGANLQDHLQIRPVFKVRGVRTLNVLASSFWGRLAIG
                     LEYALRRTGPMSMAPSQLGAFVRSDPARVHANLQYHVQPLSLRAYDQPPDTFAAFTAS
                     VCNLNPESRGTVRIRSANIADAPRIAPNYLSTDEDRRIAADSLRVTRRIVAQPALAKF
                     LPVEHLPGAQFVTDEELVREGARIATTIFHPVGTARMGSDNDPGAVLDSHLRVRGVDG
                     LRVVDASVMPTITSGNTNSPTLMIAEKAARWIIAGV"
BASE COUNT          764 a         1488 c         1481 g          768 t
ORIGIN      
        1 tttttgctat gaagacaggt gtgccaatga agaaaaatct cgtctcgctt tcggttctcc
       61 tcgccacgct cacgctgttc gcgggttgca agaccaagcc gccaccggtg gtggaaacgc
      121 cgcctcccca gaccagcgac acgaccggcg tcgaagatcc gaacgcgtcg atcggtgacg
      181 acgcccagat gggcccgacg ggcgagctgc tgtccaagcg catcgtgtac ttcgacttcg
      241 accgcgccga gatccgcgcg gattcgcaga gcgtcgtcgc cgcgcatgcg gcctatctcg
      301 cgaagaaccc gaatcagaag gtgcgcctcg aagggcatgc cgacgagcgc ggctcccgtg
      361 agtacaacat cggcctgggc gaacgccgcg ggcagaccgt gcgccgtgcg ctggcgctgc
      421 agggtgtcgc ggagacgcag ctttccaccg tgagctacgg tgaagagcgg ccggccgccg
      481 cgggcagcga cgaacaggcg tacgccgcca atcgtcgcgt cgagatcgtt tacctgaagt
      541 aatcgcaccg aggtgcccgt gaacacatcg cgattgttag gcctggccgg ggcgcttgcg
      601 ctcgtgggct gtgcggtctc acccgaggaa gatccggtga tgatccgcat gaacgacctc
      661 gatgcgcgcg tgcagcgcat cgaacgcgtg ctcacgaacc agagcctgct cgagctggcg
      721 cagcgcctcg acgccttgca gggcgaggtg cgcaacctgc gcggcgaagt cgagttgctg
      781 cagaacgaga cccagggcgg caagacgcag tcgcgcaatc tgtacggcga tctcgaaaag
      841 cggctggccg cactcgagac gctgggcgga gtggggtctg cgcccgcgcc aggggttgta
      901 ccggtgcccg tgtccggcgc gtccgagcag gcgacctacg acgccgccat ggcggccctg
      961 cgcggcggca actacgacaa ggcggtggcc acgttccgcg aggtggtcac taactacccg
     1021 gccggggatc tcgcgcccaa cgcgcagttc tggatcggcg agagttacta caccaagggc
     1081 gatctcgaga gcgccgtcac ggcctatcgc aaggtgctgg ccgactggcc gaattcgcgc
     1141 aaggcgcccg acgccatggt gaagctcggc ttctcgctgt cggacctgcg gcgcacgccc
     1201 gaggcgcgca ccacgctcga ggaagtcgtg cgcagatatc cggggaccaa tgccgcacag
     1261 ctcgccgcgg accgcctgaa gcgcctcgga agatgatcca cggtcgatga acgccgctgc
     1321 cagcgagctc ccgcccggcg ccgaacgtcg cctcaagatc acggaaattt tctactcgct
     1381 ccagggcgag gccgataccg tgggctatcc caccgtgttc gtgcggctca ccgggtgtcc
     1441 actgcgttgc cacttctgcg acaccgccta tgcgtttcac ggcggcgagt ggcgcagcgt
     1501 gggcgaagtg ctcgagacgg ttgccggctt ttcaccgcgt tacgtatgcg tcaccggcgg
     1561 cgaaccgctg gcgcagaaga attgcctgcc gttgctgacg cagctctgcg atgccggcta
     1621 tcgcgtgtcg atcgagacca gcggcgcgat gccgctcgac ggcgtcgatg agcgcgtgat
     1681 ccgcgtggtc gacgtgaaga cgccgggttc gggcgaagag cgccgcaatc gctacgacga
     1741 cctcaacctg ctgcgccccg aagagcagat caagttcgtg atctgcgatc gcgcggacta
     1801 cgagtggagc cgcgacaaac tgtctagcct gaatctcgcc gcgcgctgcc aggtgttgtt
     1861 ctcgccgagc gccgatcaac tgccggcgcg cgaactcgcg gactggattc tggccgaccg
     1921 gttgcccgtg cgattccaga tgcagcttca caaggtgctc tggggcaacg tgccaggaaa
     1981 atgaccatga cgaagcgggc cgtggtgttg ttgtcgggcg ggctggactc ggccacggtg
     2041 ctcgcgctcg cgcgggcggc cggtctcgat tgctacgcgc tctcggtgtc gtacggtcag
     2101 cggcaccagg ccgagatcag cgcggccgca cgtgtcgcgc atgcgctcgg cgcgcgcgag
     2161 catcgcatca tgcacgtgaa cctggggcgc atcggcggct cggcgctcac cgacaattcc
     2221 atcgatgttc ccgtgacgcc cggcgagggc attcccgtca cctatgttcc cgcgcgcaac
     2281 accatcatgt tgtcgctggc gctcgcctgg gccgaagtgt tgaaggccac cgaaatccac
     2341 attggcgtga acgcggtcga ctactccggc tatccggact gccggccgga attcgtggcg
     2401 gcgttccagc agctcgccac gctcgcaacc aaggcgggcg tggagggtgc gaacctgctg
     2461 atccgtgcgc cgctgatcga cttcagcaag gcgcaaatcg tgcgcgaagg agcccgtctg
     2521 ggcgtggact attcgctgac cgtctcctgt taccaggccg acgaccaggg actcgcctgc
     2581 ggacgttgcg actcctgcag gttacgtcgg gaaggctttg tttctgctgg gattcccgac
     2641 cccacgcgct acctgtcttt ccagggccaa ggcgacgaaa agatcgtgta gacttcgcgc
     2701 ccttccgggg ggtcggtagc tcagttggta gagcagcgcc cttttaaggc gttagtcgtg
     2761 ggttcgagtc ccacccgacc caccaataac aaccccgggt ccgcaattga gcgatcccga
     2821 attcgattac gtgatcatcg gcgcgggcac agcgggatgc ctgctcgcca atcgtctgag
     2881 cgcggatccc tcgcgccgcg tattgctcgt cgaagcaggt cctcctgaca actatgcgtg
     2941 gatccacatt cccgtgggct atctacgctg catcggcaat ccgcgcaccg attggcgcta
     3001 ccgcaccgaa ccggaagccg cgctccatgg ccgcgacctg ctgtatccac gcggcaagac
     3061 cttgggcggc agctccagca tcaacggcat gatctacatg cgtggccagg cgcgtgacta
     3121 cgacgcgtgg gcgcgcgcaa ccggtgatga cgcgtggagc tggccgaact cattgccgga
     3181 tttccgcgcg cacgaatgca gctttcgttg cgaaccgggc cgcgaagatc cgatgcacgg
     3241 gtcgggcggc gaatggcgcg tctccagaca acgtttgcgc tgggacatcc tcgattcttt
     3301 cgccgaagcc gccgaacagg cgggcttccc gcgcattccg gacttcaaca ccggcgacaa
     3361 cgcgggggtc agttacttcg acgtgaacca gcgcggcggt ttccgcgtga gctcggccaa
     3421 ggcgttcctc aagccggcga gggctcgcag aaacctgacg gtgtggaccg gcgcgctcac
     3481 ggagcggttg cacatcgagc gcgatggcaa cggcgcgctg cggtgttcgg gcgcgacgct
     3541 ggtccacaat ggccagcaca tccgggtgcg cgccacgcgc gagcttttgc tgtcagcggg
     3601 tgcgatcgcc acgccgcagc tcctgcagct ctcaggtctt ggccccgcgc cgctgctgcg
     3661 cgaacacggc atcgaagtgc tgctcgattc accgggcgtc ggagcgaacc tgcaggatca
     3721 cctgcagatc cggcccgtgt tcaaagtgcg cggcgtgcgt acgctgaacg tgctggcttc
     3781 gagtttctgg ggccggctcg cgatcgggct ggaatacgcg ttgcgccgca cgggccccat
     3841 gagcatggcg ccctcccagc tcggcgcgtt cgtgcgcagc gatcccgcgc gagtgcacgc
     3901 caacctccaa taccacgtgc agccgctgtc attgcgcgct tacgatcagc cgcccgacac
     3961 ttttgcggcc ttcacggcca gcgtctgcaa tctcaatccc gagagtcgcg gcacggtgcg
     4021 gatccgcagc gcgaacatcg ccgatgcacc gcgcatcgcg cccaactacc tgtcgaccga
     4081 cgaagaccgg cgcattgcgg ccgactcctt gcgcgtcacg cggcgcatcg tggcgcagcc
     4141 ggcgctcgcg aaattcctcc ccgtggaaca cctgcccggg gcgcagttcg tcaccgacga
     4201 agagctggtt cgcgaggggg cgcgcatcgc caccaccatt tttcatcccg tcggcacggc
     4261 gcgcatgggg tccgataacg acccgggcgc cgtgctcgat tcccatctgc gggtgcgtgg
     4321 cgtcgatggg ctgcgcgtcg tcgatgccag cgtcatgccg acgatcacca gcggcaatac
     4381 gaacagcccc acgttgatga ttgccgaaaa agcggcgcgc tggatcatcg ccggggtgta
     4441 gtcgcggcgt gttttgctac actgccgcgc cgtttgagtg ggtcatcgtc tagggggtca
     4501 a
//
LOCUS       DATDEU010000477         2154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1694462, whole genome shotgun sequence.
ACCESSION   DATDEU010000477 DATDEU010000000
VERSION     DATDEU010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694462"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..533)
                     /gene="rplI"
                     /locus_tag="VIV63_11390"
     CDS             complement(84..533)
                     /gene="rplI"
                     /locus_tag="VIV63_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY6125244.1"
                     /translation="MDVILLTKVANLGTIGDRVKVKSGYGRNFLLPKGKATLATPENV
                     KKFEARRAELEKAAREQFQDAESRAAAFKDFKLLITAKAGTEGKLFGSIGTADIAEAA
                     TKAGHKVARAEVRLPTGPLRTVGDHTITLHLHTDIDVQLPVVITAED"
     gene            complement(541..750)
                     /gene="rpsR"
                     /locus_tag="VIV63_11395"
                     /pseudo
     CDS             complement(541..750)
                     /gene="rpsR"
                     /locus_tag="VIV63_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(831..1235)
                     /gene="rpsF"
                     /locus_tag="VIV63_11400"
     CDS             complement(831..1235)
                     /gene="rpsF"
                     /locus_tag="VIV63_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY6125245.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMVERYRGMISAGGGAVHRHEDWGR
                     RQLTFPIAKVHKAHYVLLNIECDQKTLAELTGAFRFSDAVLRHLVVNMDEPVTEPSPM
                     AKAAQEEKEGGPRRSRDDSPFASDDDDMGVNA"
     gene            complement(1361..>2154)
                     /gene="rlmB"
                     /locus_tag="VIV63_11405"
     CDS             complement(1361..>2154)
                     /gene="rlmB"
                     /locus_tag="VIV63_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY6125246.1"
                     /translation="EESELSEKVYGLHAVRALLTRHAERVTGVTIAEQRSDPRMAEIL
                     TLAQKAGKTVKRVPPAVFKTLFGDAVHQGVYVEVQPLSVWHEEELVAAIVARLEAAEA
                     ETEKGKPPFVLVLDGVQDPHNLGACLRTADACGALAVVIPKDRAVQMNATVRKVAAGA
                     AETTPVAVVTNLARTLRLLKEAGLWIVGADAEAPRLAHETDLSGPIALVMGAEGSGLR
                     QLTRDTCDLTVRLPQQGAVESLNVSVASGMLLYEALRQRLGGKKG"
BASE COUNT          369 a          704 c          668 g          413 t
ORIGIN      
        1 gaaatatttc gcgattcccc aaaagagaac ggcgcccact tccgcgagga aggggcgccg
       61 ttcgcaacgc attgaagcgc ttatcagtct tcggcggtga tcacgaccgg cagctgcacg
      121 tcgatgtccg tgtgcagatg cagggtgatc gtgtgatcgc cgaccgtgcg caacggaccc
      181 gtgggcaggc gcacttccgc gcgcgcaacc ttgtggcccg ccttcgtggc ggcttccgcg
      241 atgtccgccg taccgatcga accgaagagc ttgccttcgg tgccggcctt cgcggtgatc
      301 agcaacttga agtccttgaa cgcggcagcg cggctttccg catcctggaa ctgctcgcgt
      361 gcagccttct cgagctcagc gcggcgcgct tcaaacttct tgacgttctc gggcgtggcc
      421 agcgtggcct tgcccttcgg cagaaggaaa ttgcgaccgt aacccgactt caccttgacg
      481 cgatcgccga tggtgccgag gttggccact ttcgttaaga gaatgacgtc catgatggcc
      541 tcaatgctga tcggtgtacg gcagcagcgc gaggtaacgc gcacgcttga tcgccgacga
      601 cagctgacgc tggtagaacg ctttcgtgcc cgtgatgcgg ctcggaacga tctttccagt
      661 ttcggtgatg tactgcttga gcgtgcccaa gtccttgtag tcgatctgct tcacgccctc
      721 ggcggtgaaa cggcagaact tgcgacggcg cgagaacgag cggttgccac cgcctccgcc
      781 gccaccacca ccaccaccgg gaccactgcg acgcggttta tcgtctttca tcaggcgttt
      841 actcccatgt catcgtcgtc gctggcgaac ggcgagtcgt cacggctgcg gcgcgggccg
      901 ccttccttct cttcctgcgc ggccttcgcc atcggcgacg gctcggtcac gggctcgtcc
      961 atgttgacga cgaggtgacg cagaacggcg tccgagaaac ggaacgcgcc ggtgagttcg
     1021 gcgagcgtct tctgatcgca ttcgatgttg aggagcacgt agtgcgcctt gtggaccttg
     1081 gcgatgggga acgtgagctg gcgacggccc cagtcttcgt gacggtgcac cgctccacca
     1141 ccggcggaaa tcattccgcg gtagcgctcg accattgctg ggacttgttc gctctggtcc
     1201 ggatgaacca ggaacacgat ttcgtaatgc ctcatgggga ctccttatgg gtaagccacc
     1261 cgtctgaggt tgacgatgtg gcaaggggac aaaaacgggc gcgaaggata atcaaacgcc
     1321 ccctgagtgg caagccgaaa atcaagcact taggccgaaa tcagcccttt tttccgccca
     1381 ggcgctggcg cagcgcctca tagagcagca ttccgctggc caccgaaacg ttgagactct
     1441 ccacggcgcc ctgctggggc aggcggaccg tgagatcgca cgtgtcgcgg gtgagttgcc
     1501 gcaggcccga accttcggca cccatgacca gcgcgatcgg tccgctcagg tcggtttcgt
     1561 gggcaagcct gggcgcctcg gcatccgcgc cgacgatcca gagcccggcc tccttgagca
     1621 gccgcagggt gcgcgcgagg ttcgtgacga ccgcgaccgg cgtggtctca gcagcaccgg
     1681 ccgcgacttt gcgcacggtg gcgttcatct gcacggcccg gtccttgggg atgaccaccg
     1741 ccagcgcgcc gcaggcatcc gccgtccgca ggcaggcgcc caggttgtgt ggatcctgca
     1801 cgccatcgag caccagtacg aatggcggct tgcccttctc cgtttcggcc tcggcggctt
     1861 ccagccgcgc gacgatcgcg gcgacgagct cttcttcatg ccagaccgaa agcggctgca
     1921 cctcgacgta aacaccctgg tgcacggcgt caccgaacag cgtcttgaac accgccggcg
     1981 gcacccgctt caccgtcttt cccgccttct gcgcaagcgt gaggatctcc gccatgcgcg
     2041 gatcgctgcg ctgctcggca atggtgacac cggtgacgcg ttccgcgtgc cgcgtgagta
     2101 gcgcccgcac tgcgtgcaga ccataaactt tttcgctcaa ttccgattcc tcga
//
LOCUS       DATDEU010000478         3993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_529228, whole genome shotgun sequence.
ACCESSION   DATDEU010000478 DATDEU010000000
VERSION     DATDEU010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529228"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VIV63_11410"
     CDS             complement(<1..608)
                     /locus_tag="VIV63_11410"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6125247.1"
                     /translation="MKREINDIATARLNELGAIVAKFSNGDGIHPAAIGGLHCIKLSE
                     PHLQLPSVYQPCVCVIVQGAKQVLLEEEIYRYTPTQFLAVSVDLPLLGQVIEASADTP
                     YLCLAIDIDARQIADLIAQSGDATWSRGETSRGLFIGELDEALLESTLRLACLLETPR
                     DIPVLAPLALREFHYRLLNSPYGAAIAQMAIAGSNSHKIAQI"
     gene            769..1500
                     /locus_tag="VIV63_11415"
     CDS             769..1500
                     /locus_tag="VIV63_11415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6125248.1"
                     /translation="MSKVILITGASSGIGEATARTLAANGHKVVLGARRTERLEKIVE
                     EVRKAGGVAEYRALDVANRDDVNAFVAFAHQKFGRVDVIFNNAGVMPVSPMNALKVDE
                     WDRIIDVNIKGVLNGIAAVLPIMEKQGSGHIISTASTGAHAVGGQFGVYCASKYAVRA
                     IMEGLRQETNQIRVTTLSPGVTESELGHDITVGDTKAAVTQLRSIALDTKAIANAVLY
                     AVSQPDDVDVNEMIIRSVRSAGHAF"
     gene            complement(1517..1717)
                     /locus_tag="VIV63_11420"
     CDS             complement(1517..1717)
                     /locus_tag="VIV63_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125249.1"
                     /translation="MKRRKEAAITLHDYRMALQNAVTWLGERYLLAEPVVRRNTVQQP
                     FFVEPKRWHEVTQSRGPGSRRH"
     gene            2345..2881
                     /locus_tag="VIV63_11425"
     CDS             2345..2881
                     /locus_tag="VIV63_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6125250.1"
                     /translation="MGGESSLCVEQERESLSVHDIIRRHHDSLIRFLRQRLRVKDDAA
                     DVAQEAYIRMMQYEGSRDIHSPSSMLFRIAINVANDLGRSEQARRVNDQTSIDGMDLA
                     CGSPTVEREIAAEQDLTLLYDAIDHLPPKCQRVFLLSRVHHMTYPQIATHCGISVKMV
                     EKHIGHALAVCMKKVGGS"
     gene            2910..3914
                     /locus_tag="VIV63_11430"
     CDS             2910..3914
                     /locus_tag="VIV63_11430"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY6125251.1"
                     /translation="MNRERTLQEAARRYARLHASDCSQDEKDATTEWLAKSPENLQAY
                     ELAERVSAGVNALAQDDRLGQKLRALADAAYTTRAVAPPRRWRVGAGLAASLLVAVLA
                     WQGSSHTVTDSPQLVVYESTGAERRSVMLDDGSTVELDVDSRIAIRMSTERRQIELVS
                     GRAVFDVAHDAGRPFSVTAAGSRTTALGTKFQVWRDDANVHVTLAEGSVAIDRDDRGF
                     TGEAWSERLSPGEQLSIDTATQRRERRVVDVNMTTSWTHGRHIFRGTPLHAAIDEVNR
                     YATRKIRLGDASLANLSVAGNFIVGDSEVIVEAFEAVLPLHAVDGGDREILLIRSHSP
                     "
BASE COUNT          758 a         1236 c         1280 g          719 t
ORIGIN      
        1 atgatctgcg caatcttgtg agagttgctc ccggcgatcg ccatctgcgc gatcgcggcg
       61 ccgtaggggc tgttcaacaa ccggtagtga aactcgcgca gcgccaatgg cgcgagcacc
      121 gggatatcgc gcggtgtctc gagcaggcac gcgagccgca aggtcgactc cagcagggct
      181 tcatcgagct cgccgatgaa caacccgcgc gacgtctcgc cgcgcgacca cgtcgcatcg
      241 ccggattgcg cgatgagatc cgcgatctga cgcgcgtcga tgtcgatggc caggcacagg
      301 tacggcgtat cggcgctcgc ctcgatgacc tgacctaaca acggcagatc cacggacacc
      361 gcgaggaact gcgtcggcgt gtagcggtag atctcctctt cgagcagcac ctgcttggcg
      421 ccctggacga tcacgcagac gcagggctgg tacaccgagg gcagctgcag gtgcggctcg
      481 gagagcttga tgcagtggag cccgccgatg gcggctgggt ggatgccgtc accgttggag
      541 aatttggcga cgatggcccc gagttcgttt agccgggcag tcgctatgtc attgatttcg
      601 cgtttcatat ggcgcccacg atacgcccgg gacgccagcg cctcaatggt ccggctccat
      661 cgtttgcagg atcaggcaat caacgtggag gagcgtgtat ccccgaaaag ccccccgcgc
      721 accagacttc tcccacgcaa tcactcaccc ctaactggag aaagcatcat gtcgaaagtc
      781 atcctcatca ccggcgcgag cagcggaatc ggcgaagcca ccgcccgcac gttggcggcc
      841 aatggccaca aggtagttct cggcgcgcgc cgcaccgagc gtctggaaaa gatcgtcgaa
      901 gaagtgcgca aggccggcgg cgtcgccgag taccgcgccc tggacgtggc caatcgcgac
      961 gacgtcaacg cgttcgtcgc cttcgctcat cagaagttcg gacgcgtgga cgtgatcttc
     1021 aacaacgcgg gcgtcatgcc ggtgtcgccg atgaacgcgc tcaaggtcga cgagtgggac
     1081 cgcatcatcg acgtgaacat caagggcgtg ctgaacggca tcgccgcggt gttgccgatc
     1141 atggaaaagc agggaagcgg gcacatcatc agcacggcgt ccacgggcgc gcatgcggtg
     1201 ggtggtcagt tcggcgtgta ctgcgcgagc aagtacgcgg tgcgcgcgat catggaaggt
     1261 ctgcggcagg aaacgaatca gatccgcgtg acgacgctgt caccgggcgt cacggaatcc
     1321 gagctgggcc acgacatcac ggtcggtgac acgaaggcgg ccgtgacaca gttgcgcagc
     1381 atcgcgctgg atacgaaggc gatcgcgaac gcggtgctct acgcggtttc gcaaccggac
     1441 gacgtggatg tgaacgagat gatcattcgg tcggtgcgca gcgcggggca tgcgttctga
     1501 tcgagacggg ccggtgtcag tgccttcggc tgcccggccc ccttgactgc gttacctcgt
     1561 gccagcgttt cggctcaacg aagaacggct gttgcaccgt gtttcgccgc accacaggct
     1621 ccgcgagcag ataacgctcg ccgagccacg tgacggcgtt ctgcaatgcc attcggtagt
     1681 cgtgcagcgt tatggcagct tctttgcggc gtttcatgtg agcctccatc gattcctgac
     1741 gattgacatt gtcggccgcc ggaactgaac agaccctgaa ggtcgaattg tccgggcggc
     1801 gggctgcagg cgggcaacac gcggtccacg ggttcgccgc gatcgaggac catggcgtcc
     1861 atcgggtagt tagtcgacac catgaagatg ccccacgccc atcggccgga ccaagacctt
     1921 gccggcgccg ccgcggtccc cgcctgtccc acggacaagc aggtttacga tggtggtcgc
     1981 ctgtgccgtc ctacacgcac ctttgcaatc aaccggaaat tcctgcacct cgctgacaca
     2041 ggacttcggc gccggcactg ctgcgatgca atctgcgtgt cacataaagt ggctacgaac
     2101 cccgctttgc gtcgggccac gcgcgatatc cccgcgacgc ggcgcgtccg gcggaaattc
     2161 acgcgcggca aaaccgcagc ttcttccaag tcttacggcg attcgccgtt tctctcactc
     2221 atgtcgtcgg tgtgtctgcc ccgtgtcggt tcaactcgcg aacggtcaat ggcatgcaac
     2281 atgcatcttt cgacgcccgg gggatcgact tgtgagcgtg cgttacatga gatccgaaga
     2341 aatattgggc ggcgaatctt cactctgcgt cgagcaggag cgcgagtcgt tgtcggtcca
     2401 cgacatcatt cgccgacacc acgactcgtt gatccgcttt ctgcgccagc gcctgcgcgt
     2461 gaaagacgat gcggcggatg tggcccagga agcctacata cggatgatgc agtacgaagg
     2521 ctctcgcgat attcattcgc cgtcttcgat gctgttccgt atcgccatca acgtcgccaa
     2581 cgatctgggg cgctcggagc aggctcgccg ggtcaacgat cagacctcca tcgacgggat
     2641 ggatctcgcc tgcggctcac cgacggtcga gcgggaaatc gcggccgaac aagaccttac
     2701 gttgctctac gacgccatcg accatctgcc gcccaagtgc cagcgcgtct ttctgctgag
     2761 ccgggtacac cacatgactt atccgcagat cgccacccat tgcggcatct ccgtgaagat
     2821 ggtcgagaag cacatcggac acgcgctggc ggtctgcatg aaaaaggtag ggggaagctg
     2881 agacccaacg tcttggtcat atggaatcca tgaatcgcga acgcactttg caagaggcgg
     2941 cgcgcaggta tgcgcggctg cacgcttctg attgctctca ggatgagaaa gacgccacca
     3001 cggaatggtt ggcaaagtct cccgagaatc ttcaggccta cgaactggcg gagcgggttt
     3061 ccgccggtgt gaatgcgctg gcccaggacg atcgattggg tcagaagttg cgcgccctgg
     3121 cagacgctgc ttacaccact cgtgcggtag cgccgccgcg acgctggcgt gtcggcgccg
     3181 gtctcgctgc gagtctcctc gtcgcggtgc ttgcctggca gggttcatcg cacacggtta
     3241 cggactcgcc gcagttggtc gtctacgaat ccaccggcgc cgagcgccgt tcggtaatgc
     3301 tcgacgacgg atccacggtc gagctcgacg tagatagccg catcgcaata cgcatgagca
     3361 ccgagcgtcg ccagatcgaa ttggtgtcag gtcgcgcggt gttcgatgta gcgcacgacg
     3421 ccggccgtcc gttctcggtc accgcggccg gctcgcgcac gacagccctg ggcacgaagt
     3481 tccaggtgtg gcgcgacgat gcgaatgtgc acgtgacgct ggcggagggt tcggtggcca
     3541 tcgatcggga tgatcgcggc ttcaccggcg aagcgtggtc ggagcgcctg agtccgggcg
     3601 agcagctcag catcgatacg gcgacgcagc gacgcgagcg tcgagtcgtg gatgtgaata
     3661 tgaccacgag ctggactcac ggccggcata tcttccgcgg tacgccgctg catgcggcga
     3721 tcgacgaggt gaaccgctac gccacccgga agatccggct cggcgacgcg tcgctcgcca
     3781 atctgtcggt ggcgggaaac ttcatcgtcg gcgacagcga ggtcatcgtc gaggctttcg
     3841 aggccgtgct gcccttgcac gccgtggacg gcggtgatcg cgaaatcctg ctgatccgca
     3901 gccacagccc gtgagccatg aggtcgcggt acgtgatgca ccatgaaatc accgccgggg
     3961 caatagccgc aatcgggagg cggactcacg gat
//
LOCUS       DATDEU010000479         3622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1986780, whole genome shotgun sequence.
ACCESSION   DATDEU010000479 DATDEU010000000
VERSION     DATDEU010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986780"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VIV63_11435"
     CDS             complement(<1..210)
                     /locus_tag="VIV63_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125252.1"
                     /translation="MQPKVKRSELDGLAEFHSLDASKVEALLELAEARPSRAEGLRFL
                     GTCLRIAGVLSLAAGLVFFVAAHWSR"
     gene            324..1646
                     /locus_tag="VIV63_11440"
     CDS             324..1646
                     /locus_tag="VIV63_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY6125253.1"
                     /translation="MTSSHIDEFDGLRVNRKTGEVAVAGKVIRLSPQYARVLLELTDN
                     AGAVVSRERLAKLLWPRGNTESEDRLNAIVRELQTALGDQADSPRYIETVPHSGFRFI
                     GALADTPTVEAPALRLRNSRRWMWWLAALPLLAVIGWWLYQRVDRSPGSQSDMASADL
                     EAALREEPDDAESWAALSTALIAAALAQLEPPGPYLDRARDAAERAVKLDETLAAAHA
                     ALGKVHTLYTRDLAKADDELARAIELDERLPEAWEGIGILRAFQGRADDALAAMSRAR
                     ELDPHSLRLVSRYGLLLFHARRYDAALSHLLPLVTAHPDLVEARSVLIRALIAKGRFD
                     EALAQSKLRTTDIPNLSDLALLYSKIGRREDALAEAQRIEARGYHGFSTSYETAVIYA
                     SLDELDKACAAIAQAAEEHSTSVGWARLDPRLDPLRGKSCFAVIEPRI"
     gene            1722..2774
                     /locus_tag="VIV63_11445"
     CDS             1722..2774
                     /locus_tag="VIV63_11445"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEY6125254.1"
                     /translation="MSKTSLIYIATQDPQKMGVTVAEFDSATGALSVPKMVIETRDPA
                     HFTLSNDGKYLYMCNTGTPGGVSAFAVENKTGALKLLNYKESKGRGPSYVSVDGSGKY
                     VLDANYGGGYVEVYSLSKDGSLDQQTAFVQHTGSSVHARQNKPFAHWFRTDPTNKFGL
                     VADLGIDQVVVYKFDAKTGTLTPNDPPFAKVPGGMGPRHLAFHPNGKWVYGIAELANE
                     VMAFNWDAGKGVLTQFQSVKTLAEGFKDPSTAAEIAVRADGKFLYASNRGEDSLVVYA
                     IDAKSGELTFKQRTPSRGKVPRYFTFDPTGKWLIVSNQDGGNVSVFSVDAKSGELAPV
                     GEPVALAKPMALEFLK"
     gene            complement(2824..>3622)
                     /locus_tag="VIV63_11450"
     CDS             complement(2824..>3622)
                     /locus_tag="VIV63_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6125255.1"
                     /translation="KGPRPVVIALHGTGGTKEGGEIVNIAVKAARAGFIGVAIDGRFH
                     GERTKAGTGAAEYNDAIALAFTSGKGHPFYYDTAWDVMRLIDYLATRKDVDAARIGLT
                     GISKGGIETYFAAAADPRVAAAVPYIGVQSFKWALDNGQWRARIATIQSAFDASAVAA
                     GKPAGSVDFVRQFYARVVPGIDGQFDGPAMLVAIAPRPLLVINGDSDANTPIAGVRLS
                     VAAAKPFYEAANASDKLQLIVQENTPHRVNPESVDAGIAWFVRWLGP"
BASE COUNT          661 a         1158 c         1167 g          636 t
ORIGIN      
        1 cctgctccag tgggcggcga cgaagaacac caggcccgcg gccaacgaca gcacgccggc
       61 aattcgcagg caggtaccga ggaaacgcag tccttcggcg cgagagggtc gcgcttcggc
      121 gagttccaac agagcctcga ccttgctggc gtccagtgaa tggaactccg ccaggccatc
      181 gagctcgctg cgtttcacct tgggctgcat tcgcgcggga gtatagccgc accgaacagc
      241 gtgacgatca ttgtgagtgc ggttcgagca ggttgtatat gccgataccg tcggtagtac
      301 cgcgcgcact atcatcggga tgcatgactt cttctcacat cgacgagttc gacggcctgc
      361 gcgtgaaccg caagaccggc gaagtggccg ttgcaggaaa agtcattcga ttatctccgc
      421 aatacgcgcg cgtactgctc gagctaaccg acaacgctgg cgcagtggtt tcgcgcgagc
      481 ggctggcaaa actgttatgg ccgcgcggga atactgaatc cgaggaccgg ttaaacgcca
      541 tcgtgcggga gttgcaaacg gctctgggcg atcaggccga ctcgcctcgc tacatcgaaa
      601 ccgtgccgca ctcgggcttt cgattcatcg gcgcactcgc tgacacaccc actgtcgagg
      661 cgccggcact gcggttacga aatagccggc gctggatgtg gtggctcgcc gcgcttccgc
      721 tgctggcagt gatcggctgg tggctgtacc aacgcgtgga tcgctcgccg ggctcgcagt
      781 ccgacatggc gtcagcggat ctcgaggccg cacttcgcga agagccggac gacgccgagt
      841 cgtgggcggc gctttccacc gcgctgattg cggccgcatt ggcgcaactt gagccgccgg
      901 gtccatacct cgaccgcgcg cgcgacgcgg cggagcgcgc ggtgaaactc gacgagacgc
      961 tcgccgcagc gcatgcggcg ttgggcaagg tgcacacgct ctacacacgc gatctggcga
     1021 aagcagatga cgaactcgca cgagcgatcg agctcgacga aagattgccg gaagcgtggg
     1081 aaggcattgg catcctgcgc gcgtttcagg gccgggccga cgacgcgctg gcggccatga
     1141 gccgtgcccg cgagctggat ccgcattcgc ttcgcctggt ctcccggtac ggtctgctgc
     1201 tgtttcacgc ccgccgatac gacgccgcgc tttcgcatct gctgccgctg gtgacggccc
     1261 atcccgatct cgtcgaagcg cgcagcgtgc tcattcgcgc gttgattgcg aaaggccggt
     1321 tcgatgaagc gctggcgcaa agcaagcttc gaactactga cataccgaac ctgagcgacc
     1381 tcgccctgct ctattcgaag atcggccgtc gcgaggacgc gctcgcagag gcgcagcgca
     1441 tcgaagcgcg cggctaccac gggttctcga cgtcgtatga gacggcggtc atctacgcat
     1501 cgctcgacga gctggacaag gcgtgcgcgg caatcgcgca ggccgccgaa gagcattcga
     1561 cttccgtcgg atgggcgcgc ctggacccca gactggaccc gctgcgcggc aagagctgct
     1621 ttgcggtcat cgagccgcgt atctgactgt ggcatgctcg cgccatgaaa acgcacgcca
     1681 tggttctcac ccttttcact tcgttagtag ccagcaccgc catgtccaaa acctcgttga
     1741 tctacatcgc cacccaggac ccgcaaaaga tgggcgtcac cgtggcggaa ttcgattcgg
     1801 ccaccggcgc cctgtcggtg ccgaagatgg tcatcgagac gcgtgatccg gcgcatttca
     1861 cgctgtcgaa tgacggcaag tatctgtaca tgtgcaacac cggcacgccc ggtggtgtca
     1921 gcgcgttcgc ggtcgagaac aagacgggcg cgctgaagct gctcaactac aaggagtcga
     1981 aaggccgtgg ccccagctac gtcagcgtcg acggcagcgg caagtacgtg ctcgatgcga
     2041 attacggcgg tggttacgtc gaggtgtatt cgctctcgaa ggatgggtcg ctggatcagc
     2101 agaccgcgtt cgtgcagcac accggctcga gtgtgcatgc gcgccagaac aagccgtttg
     2161 cgcactggtt ccgcaccgac cctaccaaca agtttgggct ggtcgccgat ctcggcattg
     2221 accaggtggt cgtttacaaa ttcgatgcga agaccggcac gctgacgccc aacgatccgc
     2281 cgttcgccaa ggtgcctggc ggcatggggc cgcggcatct cgcgtttcac ccgaacggca
     2341 agtgggtgta cggcatcgcg gaacttgcga atgaagtcat ggcgttcaac tgggatgccg
     2401 gcaagggtgt gctcacgcag ttccagtcgg tgaagacgct cgcggaagga ttcaaggatc
     2461 cgagcaccgc cgcggagatt gccgtgcgtg ccgatggcaa gttcctgtac gcatcgaatc
     2521 gtggcgagga cagtctcgtg gtttacgcca tcgatgcgaa atcaggcgag ctcacgttca
     2581 agcagcgcac gccgtcgcgc ggcaaggtgc cgcgctattt cacgttcgac ccgacgggca
     2641 agtggctcat cgtgagcaac caggacggcg gcaacgtgtc ggtgttcagc gtggacgcga
     2701 agagcgggga gcttgcgccg gtgggcgagc ccgtcgcgct cgcaaagccg atggcgctgg
     2761 agttcctgaa gtagtgagaa atggggacat tcctgatttg ctagcaaaca gggacgcgcc
     2821 tcactagggc ccgagccacc gcacgaacca cgcgatcccg gcgtcgacgc tttccgggtt
     2881 cacgcgatgc ggcgtgttct cctgcacgat gagctgcaac ttgtccgagg cattcgctgc
     2941 ttcgtagaac ggcttcgccg cggccaccga caaccgcacg cccgcaatcg gcgtgttcgc
     3001 atccgaatcg ccgttgatca ccagcaacgg ccgcggcgcg atcgccacga gcattgccgg
     3061 tccgtcgaac tggccgtcga tgccgggcac cacgcgcgcg tagaactggc gcacgaagtc
     3121 gacactgccc gcgggcttgc cggccgccac cgctgacgca tcgaacgccg actggatagt
     3181 cgcgatgcgc gcgcgccact gtccgttatc cagcgcccat ttgaaactct gcacgccgat
     3241 atacggcacg gccgcggcca cgcgcgggtc cgcggcggcg gcgaaatagg tctcgatgcc
     3301 gcccttggaa attcccgtga ggccgatgcg cgcggcgtcc acatccttgc gcgtggcgag
     3361 gtagtcgatg agccgcatga cgtcccacgc ggtgtcgtaa tagaacgggt gtcccttgcc
     3421 gctcgtgaac gcgagggcga tggcatcgtt gtattccgcg gcgccggtgc ccgccttcgt
     3481 tcgctcgccg tggaaacgcc cgtcgatcgc cacgccgatg aaccccgcgc gcgccgcctt
     3541 gaccgcgatg ttcacgatct cgccgccctc tttggtaccg cccgttccgt gcagcgcgat
     3601 gacaaccggg cgcgggccct tg
//
LOCUS       DATDEU010000480         3197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1694926, whole genome shotgun sequence.
ACCESSION   DATDEU010000480 DATDEU010000000
VERSION     DATDEU010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694926"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VIV63_11455"
     CDS             complement(<1..930)
                     /locus_tag="VIV63_11455"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEY6125256.1"
                     /translation="MSNDLIRRYVTRSLPIVAALTMASPAAYAWDFQNDSGTFSGSWD
                     TTLTYGQAWRIESRDCRLIAIADGGCGRSPNIDDGNLNYDTDMYSRAIKVLTELSLNY
                     RGVGAFVRASGLYDDAAGDTARTPLTDSSEDLVQGYTRLLDAFVYAKFDLGDMGAELR
                     VGRQVVSWGESTFIQNGLNVINHFDVSALRVPGSELKEGFLPQEMVNFSLQFNDNWSA
                     QAIYITDWNATVPEPAGSYFSANDFAPVGGSKVVLGFGSFSDQGVDFRPLGGPYITNF
                     QTVNRLPTIKASDDGQYGFNLKDFATDFNNGTEF"
     gene            complement(1150..>3197)
                     /gene="plsB"
                     /locus_tag="VIV63_11460"
     CDS             complement(1150..>3197)
                     /gene="plsB"
                     /locus_tag="VIV63_11460"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HEY6125257.1"
                     /translation="GSWLRLLFAENWALTSRVRKFFSVLINGRNVMVEMGEPLSLRSL
                     LDAAPAGDQARRVTRILRGILRRQRAIRIGPDLSHRRTIVAQVLRARAVRAVVASEAR
                     EKHDTYRPGLLQARKYAFEIAANYSHSFVQIAEKLLGRVWNRVYDGVKINHAGTLKDV
                     SEGNEVVYVPCHRSHMDYLLMSYIIYHQGYALPHIAAGINLNIPVVGRFLRKGGAFFI
                     RRSFGGNALYTVVFMKYLAAIMARGHSIEYFIEGGRSRTGRLLHPKTGMLSMTVRSFL
                     RDPVRPVVFVPVYFGYERIVEANTYISELSGAPKKKEGWLDLLLSLRVLRERFGTVHV
                     NVGEPIRLNDLLDLQLPGWREKRFDDDTRSPALNVLIGELAVTIMRGINAAAAVTPIN
                     LLATCLLASPRGALPESALLRQLELYLKLLRASPYGPRVTVTDASPAEIVVYGESLKI
                     ISRVPHKLGDIVKMSDESAQLVAYYRNNVLHLFALPSLVACAFIGNSMLRTEDIQRLA
                     WRIYPYVASELFLRWREEELAKVVDDVLAALAELGVLRPNEDRTVWMRPPPDSPAALQ
                     LSMLSSATIQTIERYYLAIALLLKAGTGVMNQKTLEQQCHLAAQRINMLYGFNSPEFF
                     DRALFENFIDLLRERGVLKAGEGGNLEFDEVLVRVAADAQLVLSEQIRHSILQVTQV"
BASE COUNT          570 a          981 c         1080 g          566 t
ORIGIN      
        1 gaattccgtg ccgttgttga aatccgtcgc gaagtccttg aggttgaatc cgtactggcc
       61 gtcatccgag gccttgatcg tcggaagccg attcacggtc tggaagttgg tgatgtacgg
      121 accgccgagc ggccggaagt cgacaccctg gtcggagaag gaaccgaagc ccagcaccac
      181 cttgctgccg ccgacgggcg cgaagtcgtt ggcgctgaag tacgaacccg ccggttccgg
      241 gaccgtggcg ttccagtcgg tgatgtagat agcctgcgcc gaccagttgt cgttgaactg
      301 caggctgaag ttgaccatct cctgcggcag gaacccttcc ttgagctccg aacccggcac
      361 gcgcagcgcc gagacgtcga agtggttgat gacgttgagg ccgttctgga tgaacgtgct
      421 ttcgccccag ctcaccacct ggcggccgac gcgcagctcc gcgcccatgt cccccaggtc
      481 gaacttggcg tagacgaacg cgtcgagcag gcgggtgtag ccctgcacca ggtcttcgga
      541 ggagtcggtc agcggggtcc gcgcggtatc gccggcggcg tcgtcataga gccccgacgc
      601 gcggacgaat gcgccgacgc cgcggtagtt cagggacagc tcggtcagca ccttgatggc
      661 gcgcgagtac atgtccgtgt cgtagttgag gttgccgtcg tcgatgttcg gcgaacggcc
      721 gcaacctccg tcggcgatgg cgatgagccg gcaatcgcgc gattcgatgc gccaggcctg
      781 tccgtaggtc agcgtggtgt cccagctacc cgagaaagtg cctgaatcgt tctggaagtc
      841 ccaggcgtag gccgcgggtg acgccatggt gagcgccgct acgatcggaa gcgaacgcgt
      901 cacgtatcgc cggatcagat cgttactcat taatttcccc tgagaccgcg gaaaggctgc
      961 gagtcgcgag gtagggagca gcagtaccgc ttggccttat cgtcttagta ttcctccgcg
     1021 ctgctgcctc ccctcgcgga cgcgtaggcg cggacgtgtt gcgcgcgagt atagcagcgg
     1081 gttcccgccc taaggctagg ccggtgcgtc cgccggggat tccctacctg ctaggaagct
     1141 ctgcaaggat cagacctgcg tgacctgcag gatgctgtgc ctgatctgtt cggacagcac
     1201 cagctgtgca tcggcggcca cgcggaccag cacttcgtcg aactcgaggt tgccaccctc
     1261 gccggccttc aggacgccgc gttcgcgcag caggtcgatg aaattctcga acagggcgcg
     1321 gtcgaaaaat tccggcgagt tgaatccgta gagcatgttg atgcgctggg cggccaggtg
     1381 gcactgctgt tcgagcgtct tctggttcat cacgccggtg ccggccttga gcagcagcgc
     1441 gatcgccagg tagtagcgtt cgatggtctg gatagtcgcc gacgacagca tcgacagctg
     1501 caaggcggcc ggcgaatccg gtggcggccg catccacacc gtccggtctt cgttaggccg
     1561 cagcacaccg agttcggcca acgcagcaag aacgtcatcc accactttcg ccagctcttc
     1621 ctcgcgccag cgcaggaaaa gctcggacgc gacgtacggg tagatgcgcc aggccaggcg
     1681 ctggatgtcc tctgtccgca acatggaatt tccgatgaac gcacaggcga ccagcgacgg
     1741 cagcgcgaac aggtgcagga cgttgttgcg gtagtaagcg acgagttgcg cgctctcgtc
     1801 gctcatcttg acgatgtcgc cgagcttgtg aggcacgcgc gagatgatct tgagcgactc
     1861 gccatacacc acgatttccg cggggctggc gtcggtgacc gtgacccgag gtccgtatgg
     1921 cgaagcgcgt aacagcttga gatagagctc gagctgacgc agcagcgcgc tctcgggcag
     1981 ggcgccacgc ggtgaggcga gcaggcacgt ggccagcagg ttgatggggg tgaccgccgc
     2041 ggcggcgttg atcccgcgca tgatggtcac ggcgagttcg ccgatgagca cgttcagcgc
     2101 gggcgatcgg gtgtcgtcgt cgaatcgttt ctcgcgccag cccggcagtt gcaggtcgag
     2161 aaggtcgttg agccggatcg gctcgccgac gttgacgtgc acggtgccga accgctcgcg
     2221 gagcacgcgc agggacagca gcagatcgag ccagccctcc ttcttcttgg gcgcgcccga
     2281 caattccgag atgtaggtgt tggcttcgac gatgcgttcg tagccgaagt acaccggcac
     2341 gaacaccacc ggccgcaccg ggtcgcgcag gaagctgcgc accgtcatcg acaacatgcc
     2401 ggtcttgggg tggagcagcc ttccggtacg cgatcgcccg ccctcgatga aatattcgat
     2461 ggagtgccca cgcgccatga tcgcggcgag gtacttcatg aagacgacgg tgtacaacgc
     2521 gttgccgccg aaactgcggc ggatgaagaa cgcgccgccc ttgcgcagga agcggccgac
     2581 caccgggatg ttgaggttga tgcccgcggc gatgtgcggc agcgcgtatc cctggtggta
     2641 gatgatgtac gacatcagca ggtagtccat gtggctgcga tggcagggca cgtagaccac
     2701 ttcgttgcct tccgacacgt ctttcagcgt gccggcgtga ttgatcttca cgccgtcgta
     2761 gacgcggttc cacacgcggc ccagcagctt ctcggcgatc tgcacgaacg aatgcgagta
     2821 gttagcggcg atctcgaacg cgtacttgcg ggcctgcaac aggccggggc gataggtgtc
     2881 gtgtttctcg cgcgcttcgg aggcgaccac ggcgcgcacc gcccgggcac gcaggacctg
     2941 ggcaacgatg gtgcggcgat gggagaggtc cggaccgatg cggatggcgc gctgccgccg
     3001 caggattccg cgcaggatgc gcgtgacgcg gcgcgcctgg tcgcccgcgg gcgcggcgtc
     3061 gagcagcgaa cgcaggctga gtggctcgcc catctccacc atcacattgc ggccgttgat
     3121 gagcaccgaa aagaatttgc gcacgcgcga ggtcagcgcc cagttctcgg cgaacagcag
     3181 gcgcagccac gagccct
//
LOCUS       DATDEU010000481         4562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_235388, whole genome shotgun sequence.
ACCESSION   DATDEU010000481 DATDEU010000000
VERSION     DATDEU010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235388"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VIV63_11465"
     CDS             <1..503
                     /locus_tag="VIV63_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125258.1"
                     /translation="YTGYERPFKNIVAPDPRDHKLGHLVGTADLMAQMADRCYLEKCR
                     DRLYAEFVLAGVALPIGENGHVKVRYASGLDLLRQTPKFVFDMRAKRLDGEFNRAYRY
                     LEVLYGGRNPYMESIDRNLEYLQRILRSENWRLLRRQPPVFTGVEDSMATTRTLMLGA
                     IKKVWG"
     gene            complement(507..1232)
                     /locus_tag="VIV63_11470"
     CDS             complement(507..1232)
                     /locus_tag="VIV63_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY6125259.1"
                     /translation="MAGTLEFTLDQLTGRTRGHLSELADPPCALHNEVMAPFLAMRAA
                     AAADGIDLRAFSSFRDFDRQLGIWNGKFRGERPMQDRQGRPLDALSLTPDMRVTAILW
                     WSALPGASRHHWGTDFDVLDANAMPAGYKLQVVPDEYGPGGPFHRLTTWLDARMHAFG
                     FFRPYSTDRGGVSPEPWHLSHAPVAERAKAKFSAGRLRPVLAEAAIEGKEAVLAALDR
                     HYANYVDNIDAAPEAALLSPRLA"
     gene            1374..3878
                     /locus_tag="VIV63_11475"
     CDS             1374..3878
                     /locus_tag="VIV63_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6125260.1"
                     /translation="MTIASFKSKYIAVAVAAAIAVSLAVGAVMVYENQTQHSALLDTA
                     AADTRSRIEGELNLRAGELARHIAERVADEVLRNERDQFAGALETFQRDETLLGVILR
                     DTGGSELYAWRRPGDTEGGITRSVITPVRAEVQAMPGVVAPQTVGEVEIEIRSLEGAP
                     ESSAARTHFDAVERSQMRQALLLAGALGLIALAVGLTLAWWAGRRVHAPISTLIKSAD
                     RIAQGDYSRPMEVGRRDELGELQAALERMRQKLRQTTINKNYLTNVLGSMTDAVFVTS
                     PDGVIRMANQSACKLLAWGEEELVGRSIVSIFDERERAEFDMLRASQDTRETVVKTRQ
                     GQTIPVAITGSQITTDDPQYQGTIFVVRNVTERKRAERRIRYLARYDALTKVPNRMQF
                     QHLLQQAIARAIRNKTSLALLYLDMDRFKEINDTFGHAAGDRTLEILTERLTRALPQE
                     TTIGRLAGDEFAMFVDDLAADADNRGTVAQLARSVLAEVGRAFQLQEHEVFLTASIGV
                     AFCTRDAENVIDLIRNADAAMYHSKQNGGNSFAFYSPEMNAAAVERLMLKSKLRRSLE
                     RDEFLMHYQPKVDLRDGRIVGAEALLRWRLPGHGDIPPSHFIPLAEETNLILEVGEWV
                     LNRVCQDYRDLQQTVTTPGRISLNLSLKQLRQASFILRFRSVFRKHGVSPTCFELEIT
                     ETTLMADSKRTLHLLDELYAMGLHLSIDDFGTGYSSLSALQQFPIGTLKIDQSFVRDV
                     DDDESDATIVRTVIEMGRSLKMNVIAEGVETVGHLHFLRRHQCGFGQGKLFGEPVTLE
                     ELRLLLARQQETGPAFAGLIAQSASLDNKIAKPRSV"
     gene            complement(3911..4549)
                     /locus_tag="VIV63_11480"
     CDS             complement(3911..4549)
                     /locus_tag="VIV63_11480"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125261.1"
                     /translation="MRNRNSWAFALLLVMPGLAPAAEEKASASDKTVCADTFKDVDIR
                     ELIEKVAKRTGKQFVIDPRVRAGIPLVGLTENDVDYARLLAILTLHQFVAYESGGVVK
                     VTVDAVSRQLPIPVTSEVPPKALDDELVSLVIQVKSACAAHLVPIVRPLMPQSAHLAA
                     MSPNALVIVDRAANARRVVEVIERLDKAAPTGQKCPAETGWTPAKAEKPEKK"
BASE COUNT          824 a         1571 c         1421 g          746 t
ORIGIN      
        1 actacacggg ctacgagcgg ccgttcaaga acatcgtcgc gccggatccg cgcgaccaca
       61 agctcgggca cctcgtcggc accgccgatc tcatggcgca gatggccgat cgctgttatc
      121 tcgaaaaatg ccgtgatcgc ctgtatgccg agttcgtgct cgccggcgtg gcgctgccga
      181 tcggcgagaa cggtcacgtg aaagtgcgtt acgcctcggg cctggatctg ctgcggcaga
      241 cgccgaaatt cgtgttcgac atgcgcgcca agcggctcga cggcgagttc aaccgcgcgt
      301 atcgctatct cgaagtgctg tacggtggac gtaacccgta catggaatcc atcgaccgca
      361 atctcgagta tctgcagcgc atcctgcgca gcgagaactg gcgcctgctc cggcgtcagc
      421 cgccggtgtt cacgggcgtc gaagattcca tggccaccac gcgcacgctg atgctcggcg
      481 ccatcaagaa agtctggggc tgagcctcag gcgagcctgg gcgacagcag tgccgcctcc
      541 ggcgccgcat cgatgttgtc cacgtagtta gcgtagtggc ggtcgagcgc cgcgagcact
      601 gcttcctttc cttcgatggc cgcctccgcg agcacggggc gcaatcttcc tgccgagaac
      661 ttcgccttcg cgcgctcggc caccggcgca tgggacagat gccacggctc gggcgaaacg
      721 ccaccgcggt cggtgctgta aggccggaag aagccgaagg cgtgcatgcg cgcatcgagc
      781 caggtggtta gacgatggaa cggaccgccg ggcccgtatt cgtccggcac cacctgcagc
      841 ttgtatcccg ccggcatcgc gttcgcgtcc agcacatcga agtccgtccc ccaatggtgc
      901 cggctcgctc ccggcaaagc cgaccaccac aggatcgcgg tcacacgcat atcgggggtc
      961 agggacaacg catcgagcgg ccgtccctgc cggtcctgca tggggcgctc accccggaac
     1021 ttgccgttcc agatgcccag ttgccggtcg aaatcgcgaa agctggagaa ggctctgagg
     1081 tcgatgccat cggccgctgc cgcggcccgc atcgccagaa agggcgccat gacctcgtta
     1141 tgcaaggcac aaggcgggtc ggccagctcc gacaggtgac cgcgggtacg gcccgtgagc
     1201 tgatccagcg tgaattccag ggttcctgcc atgcgccggc tatcctaccg gagggcggga
     1261 cccgtctttc tgcggaacaa atcgacattg cgcccctgtc ggtgacaggc gaaggtggcc
     1321 gctgccggaa accgacgggt aagattgctg caaataagaa cccccgggat tccgtgacta
     1381 tcgccagctt caagagcaaa tacatcgccg tcgccgtggc agccgccatc gcggtgtcac
     1441 tcgcggtggg cgcggtcatg gtctacgaga accagacgca gcattcggcc ctgctcgaca
     1501 cggccgccgc cgacacgcgc agccggatcg agggcgaact caacctgcgc gccggggagc
     1561 tggcacggca tatcgccgag cgggtcgccg acgaggtcct gcgcaacgaa cgcgaccagt
     1621 tcgccggagc actcgaaacc ttccagcgcg acgaaaccct tctcggtgtc atcctgcgcg
     1681 acaccggggg cagcgagctc tacgcgtggc gccgtcccgg cgacaccgag ggtggaatca
     1741 cgcgtagcgt catcacgccc gtgcgtgccg aagtgcaggc catgcccggc gtggtcgcgc
     1801 cacagaccgt cggcgaagtg gaaatcgaga tccgctcgct cgagggcgcg ccggaatcca
     1861 gcgccgcacg cacccatttc gacgccgtcg aacgctcgca gatgcgccag gcgctgctgc
     1921 tcgccggagc gctcggcctg atcgcgctgg ccgtcggcct gacgctcgcc tggtgggccg
     1981 gccgccgggt ccatgccccc atctccacgc tcatcaagag cgccgaccgt atcgcccagg
     2041 gcgactactc gcggccgatg gaagtcggcc gacgcgacga gctcggcgaa ctgcaggcag
     2101 cgctcgagcg catgcgccag aagctgcgcc agaccacgat caacaagaac tacctgacca
     2161 acgtgctcgg cagcatgacc gacgcggtgt tcgtgacctc gcccgacggc gtgatccgca
     2221 tggctaacca gtccgcctgc aagctgctcg cctggggcga agaagagctg gtcggccgga
     2281 gcatcgtttc gatattcgac gaacgcgagc gcgccgaatt cgacatgttg cgcgcctcgc
     2341 aggacacgcg cgaaaccgtg gtgaagacgc gccaggggca gacgattccc gtcgccatca
     2401 ccggttcgca gatcaccacc gacgatccgc agtaccaggg caccatcttc gtggtgcgca
     2461 acgtcaccga gcgcaaacgc gccgagcgcc gcatccgtta tctcgcgcgt tacgacgcac
     2521 tgaccaaagt gccgaaccgc atgcagttcc agcatctgct gcagcaggcg atcgcgcgtg
     2581 ccatccgcaa caagaccagc cttgcactgc tgtatctcga catggatcgt ttcaaggaga
     2641 tcaacgacac gttcggccac gcggccggcg atcgcacgct cgagatcctc accgaacggc
     2701 tgacgcgcgc gctgccgcag gaaacgacga tcggccgcct cgccggcgat gagttcgcga
     2761 tgttcgtcga cgatctcgcc gccgatgccg acaaccgcgg caccgtcgcc cagctcgctc
     2821 gcagcgtgct cgccgaagtg ggccgcgcgt tccagctcca ggaacacgaa gtgttcctca
     2881 ccgccagcat cggcgtggcc ttctgcacgc gcgacgcgga aaacgtcatc gacctcatcc
     2941 gcaacgcgga tgcggccatg taccactcca agcagaacgg cggaaattcg ttcgcgttct
     3001 attcgccgga gatgaacgcg gccgcggtcg agcggctgat gctgaagagc aagttgcgcc
     3061 gctcgctcga gcgcgacgag ttcctcatgc actaccagcc caaggtggac ctgcgcgacg
     3121 gccgcatcgt cggcgccgag gcactgctgc gctggcgcct gccgggccac ggcgacattc
     3181 cgccctctca cttcattccg ctcgccgaag aaaccaacct catcctggaa gtgggcgaat
     3241 gggttctgaa ccgcgtgtgc caggactatc gcgacctgca gcagacggtc accacgccgg
     3301 gccgcatttc gctgaacctg tcgctcaaac agcttcgcca ggcgagtttc atccttcgtt
     3361 tccggtcggt gttccgcaag cacggcgtct cgcccacctg cttcgagctc gagatcaccg
     3421 aaaccacgtt gatggccgac tccaagcgca cgctgcacct gctcgatgag ctctatgcga
     3481 tgggcctgca cctgtccatc gacgacttcg gcacgggtta ttcgtcgcta tcggcgctgc
     3541 agcagttccc gatcggcacg ctcaagatcg accagtcgtt cgtgcgcgac gtggacgacg
     3601 acgaaagcga cgccaccatc gtgcgcaccg tcatcgagat gggccgcagc ctcaagatga
     3661 acgtgatagc cgagggcgtc gagaccgtcg gccacctgca tttcctgcgc cgccaccagt
     3721 gcggcttcgg tcagggcaag ttgttcggcg aacccgtcac gctcgaagag ctgcgcctgc
     3781 tgctcgcacg acagcaggag accggcccgg cgttcgccgg gctcatcgcg cagtccgcga
     3841 gcctcgacaa caagatcgcc aagccgcgca gcgtctgaat atccgcgcgc ggcacaacgc
     3901 cgcctgctaa ctacttcttt tccggcttct cggccttggc cggcgtccac cccgtttccg
     3961 ccgggcactt ctggccggtg ggcgccgcct tgtcgagccg ctcgatcact tcgacgacgc
     4021 ggcgcgcatt cgccgcacga tcgacgatca cgagcgcgtt cggcgacatc gccgcaaggt
     4081 gcgcggactg gggcatgagc gggcgcacga tcggcaccag gtgcgcggcg cacgccgact
     4141 tcacctggat gaccagcgag accagttcgt cgtcgagcgc cttgggcggg acttcgctgg
     4201 tcaccgggat gggcagttgc cgcgagacgg catccacggt cactttgacc acgccgcccg
     4261 actcgtaggc gacgaactga tgaagcgtga ggatcgccag cagccgggcg tagtccacgt
     4321 cattctcggt gagacccacg agcgggatgc cggcgcgcac acgagggtcg atcacgaact
     4381 gcttgccggt tcgcttcgcg accttttcga tgagttcccg gatgtcgaca tccttgaagg
     4441 tgtcggcgca aacggtcttg tcgctggccg atgctttttc ttcggccgcc ggcgccagcc
     4501 ccggcatcac taacaggagc gcgaacgccc acgaatttcg atttctcatg ctcctactcc
     4561 ct
//
LOCUS       DATDEU010000482         4711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_90404, whole genome shotgun sequence.
ACCESSION   DATDEU010000482 DATDEU010000000
VERSION     DATDEU010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90404"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..313
                     /locus_tag="VIV63_11485"
     CDS             50..313
                     /locus_tag="VIV63_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125262.1"
                     /translation="MKLNVALAASIAALALMTACAKKDAEVAAPAEAAPAATEAAAAA
                     TDAAAAATDAAGAATDAAAVATDAAAAAPAAEPAPAPAEAPKQ"
     gene            409..939
                     /locus_tag="VIV63_11490"
     CDS             409..939
                     /locus_tag="VIV63_11490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6125263.1"
                     /translation="MTVSVRDARGAARDREWIESVYRDYLDDLSPLNTGVFSATSLGE
                     IGYSESDQLARWFGDSTVTPLVILRGIEPVGFAMVARGGTTPGKPPVDYRMAEFFVAR
                     TFRRRGVGHLAVELILNRFAGRWEITEYLRNPVAVSFWRRVVANYTQGRFQERVLNGE
                     VRQVFESGISRAQKPA"
     gene            986..2653
                     /gene="ggt"
                     /locus_tag="VIV63_11495"
     CDS             986..2653
                     /gene="ggt"
                     /locus_tag="VIV63_11495"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6125264.1"
                     /translation="MEGGFAGSRPSGSLVPTRSEVIATQGMAATSHPLASQVALDVLK
                     RGGTAVDAAIAANATIGLMEPTGNGVGGDLFAIVWDAKSKKLHGLNASGRSPKSLTLE
                     KLRDELKKLNSPTIPRTGPLPVSVPGAVDGWFELHGKFGKLPMKDLLAPAIAYARNGF
                     PVTEIIAEGWARNARNLQKFPGFAETYMPNGRVPAKGDVFRNPLLANTLTAIADGGRD
                     AFYQGDIARRIEAYMRANGGYLTAADLAAHRSEWVEPVSTNYRGYDVWELPPNTQGVA
                     ALQMLNILEAYDLKSLGFGSAEYLHLFVEAKKLAFEDRARYYADPEFAKIPLKGLLSK
                     EYAAKQRALIRLDRAALEYPAAPKPVDEGDTIYMTVADSAGNMVSLIQSNYRGMGSGM
                     TPSGCGFILQDRGELFSLQDGHANVYAPGKRPFHTIIPAFVTKDGKPWLSFGLMGGAM
                     QPQGHVQVVVNLIDFGMNLQEAGDAPRARHDGSSEPTGEVMKDGGEVVLEVGASPATV
                     KALEARGHKVTVTNDGDFGGYQAIMRNADGVYFGASESRKDGAAQGY"
     gene            complement(2805..>4711)
                     /locus_tag="VIV63_11500"
     CDS             complement(2805..>4711)
                     /locus_tag="VIV63_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125265.1"
                     /translation="GITAQAAPQKASFARDVSSTQWPLADFDPQLPIDWTDAEFLVVE
                     FRSSTSQRFELGLISDEGNVSKRIHPFPGVWVRASIPLRYFREGLGDADELASTVNQP
                     RNSYWINIEAGGHAPVLHVRAISLTMRYPARAATVEIRKISLAKTDPGDAVLDDGAPL
                     VDDFGQYVHADWPGKARSYPDLVREWSAENRMLVPKGKIQECRYGGYPAGRRKSTGFF
                     RVEKINDRWWFVDPEGCRFYSTGVNGAGAEPPRTQIVGRSKLFASIPSSAQFPAPGTE
                     LDPLRDPVSFYAANLEKRFGSEWQGPSAQLTSRRMRAWGLNTAYGPSLNDSQPRGAAL
                     RQPYVFPLRGWQQSQGAIMGLPDVYSEAFAKRVDAEAAQQLAPRKSDPWMIGYFIGNE
                     PPWPARESQLVDLVLAGPASSMQQRFKSELAKGDTPAIRKALVHAAFTRYLEIVNAAV
                     KRHDPDHLNLGIRFGGTPPDEVIALARGFDVYSMNKYRWAPPKDFIDRVYAIQKLPIL
                     IGEFHFGVPERGLAPGLVQAMNQSERGVAYSYYVEHAAAHPAIVGTHWYQWIDQPVTG
                     RRDGENYNIGWIDVTDRPYPELVAAARASHAKIDDIHSGRVAPTTRKPKASEFGTPED
                     SIHLGIPAIQ"
BASE COUNT          789 a         1514 c         1535 g          873 t
ORIGIN      
        1 acgtttgttt ggtctcactt accactagat atttttgggg tacccctaca tgaaattgaa
       61 cgttgcactc gccgcctcga tcgcggccct cgcgctgatg actgcttgcg ccaagaaaga
      121 cgctgaagtt gccgctcctg ccgaagccgc tccggctgcg acggaagctg ccgctgccgc
      181 cacggacgct gccgctgccg ccacggacgc cgctggcgcc gctacggacg ctgccgctgt
      241 cgccacggac gctgctgctg ccgctccggc cgcggaaccg gctccggctc cggccgaagc
      301 tccgaagcag taatcgactg attattgctt cgagccgtct ggctcaatga cacggcccgg
      361 gcagtacctt aacgtctgcc cgggctttgt cttttctgga ggggatgcgt gacagtcagt
      421 gtccgtgatg cgcgaggcgc cgcccgcgac cgcgagtgga tcgaaagcgt ctaccgcgac
      481 tacctcgacg atctttcgcc gttgaatacg ggcgtgttca gcgcgacctc gctcggcgaa
      541 atcggttaca gcgagtcgga ccagctggcc cgttggttcg gcgactccac cgtcactcct
      601 ctcgtcatcc tgcgcggcat cgagcccgtc ggattcgcca tggtggcgcg cggtggcacg
      661 acgcccggca aaccgccggt cgactaccgc atggcggaat ttttcgtggc gcgcaccttt
      721 cgccgccgcg gcgtgggaca tctcgccgtc gaactcatac tcaacaggtt cgccgggcgg
      781 tgggagatca ccgagtacct gcgcaatccg gtcgccgtgt ctttctggcg gcgcgtcgta
      841 gcaaactaca cccagggtcg ttttcaggag cgcgtcctca atggcgaagt ccgtcaggta
      901 ttcgaatcgg gcatctcgcg cgcgcagaaa cctgcctgac ccgcgccgct tcattgcctg
      961 cgttgcgctg ctgtttgtca tgaccatgga aggcggattc gccgggtcgc gccccagcgg
     1021 ctcgctggtc ccgacgcgtt ccgaagtcat cgccacgcag ggcatggccg ccaccagtca
     1081 tccgctcgcg agccaggtgg cgctcgacgt cctcaagcgc ggcggcacgg ctgtcgacgc
     1141 tgcaattgcc gcgaacgcca ccatcggtct catggagccc acgggcaatg gcgtgggcgg
     1201 cgacttgttt gccatcgtct gggatgcgaa gtcgaagaag ctccatggtc tgaacgccag
     1261 cggccgctca ccgaaatcgc tcacactcga gaagctgcgt gacgagctca aaaaactgaa
     1321 ctcgcccacc attccgcgca ccggacctct gcccgtgtcg gtgcccggtg cggtggatgg
     1381 ttggttcgag ctgcatggca agttcggcaa gttgcccatg aaagatctgt tggctcccgc
     1441 catcgcctac gcacgcaacg gatttccggt cacggaaatc atcgccgagg gctgggcgcg
     1501 caatgcgcgc aatctgcaga aattccccgg ctttgccgaa acctacatgc cgaacggccg
     1561 tgtgcctgcc aagggcgacg tgttccgcaa tccccttctc gccaacacgc tcacggccat
     1621 cgctgacggc ggccgcgatg ctttctacca gggcgacatc gccagacgca tcgaagcgta
     1681 catgcgcgcc aacggcggtt atctcaccgc ggccgatctc gcggcgcatc gttccgaatg
     1741 ggtcgagccc gtatccacta actaccgcgg ctacgacgtc tgggagttgc caccgaacac
     1801 gcagggcgtg gcggcgctgc agatgctcaa catcctcgag gcatacgacc tcaagtccct
     1861 gggtttcggc agcgcggaat acctgcacct gttcgtcgaa gccaagaagc tggcgttcga
     1921 ggaccgcgcc cggtactacg ccgatcccga attcgcgaag attccgctca agggcctgct
     1981 gtccaaggag tacgccgcga aacagcgtgc gctcatcagg ctggaccgcg cggcgctcga
     2041 gtatccggcg gcgccgaagc cggtcgatga aggcgacacc atctacatga cggtggccga
     2101 ttccgccggc aacatggtct cgctcatcca gagcaactac cgcggcatgg gatcgggcat
     2161 gacgccctcg ggctgcggct tcatcctgca ggatcgcggc gaactcttct cgcttcagga
     2221 tggccacgcc aacgtctatg cgccgggaaa gcggccgttt cataccatca ttcccgcctt
     2281 cgtgacgaag gacggcaagc cctggttgtc gttcggtctc atgggcggtg cgatgcagcc
     2341 gcagggccac gtgcaggtcg tcgtcaatct catcgacttc ggcatgaacc tgcaggaagc
     2401 cggcgacgcg ccgcgagcgc gtcacgacgg ttcgagcgag ccgacgggcg aagtcatgaa
     2461 ggacggcggc gaggtggtgc tcgaagtggg cgcgtcaccg gcgaccgtga aagcgctcga
     2521 ggctcgcggc cacaaggtca ccgtcaccaa tgacggcgat ttcggtggct accaggccat
     2581 catgcgcaac gccgacggcg tctatttcgg cgcgtccgaa tcccgcaagg acggcgccgc
     2641 gcagggttac taggttccgc ggtaactggg ctccgccgga accggattgt caggcctggg
     2701 tactcaccac cgttggcgtc ggtggtgcgg accgcacgag ccttgtttac cgattgcgag
     2761 cgctcgggca atagcagggg ggcgtgccat cgacgccagc actcctactg gatcgccggg
     2821 attcccaggt ggatcgaatc ttccggcgtt ccgaactccg acgccttcgg cttgcgcgtc
     2881 gtcggcgcga ctcttcccga atgtatgtcg tcgatcttcg cgtgtgacgc cctggccgcc
     2941 gcaaccagct ccgggtaggg tcggtcggtg acgtcgatcc agccgatgtt gtagttctcg
     3001 ccgtcgcgcc ggccggtcac gggctggtct atccactggt accagtgtgt gccgacgatg
     3061 gcgggatgtg cggctgcgtg ctcgacgtag taagagtagg cgacgccgcg ctcgctctga
     3121 ttcatggcct gcacgagacc cggggccagt ccgcgttcgg gcacgccgaa atgaaactcg
     3181 ccgatcagga tgggaagttt ctggatggcg tatacgcgat cgatgaagtc tttcggtggc
     3241 gcccagcggt acttgttcat gctgtagaca tcgaagccac gcgccagcgc gatgacctcg
     3301 tcgggcggcg taccgccgaa acggatgccg agattcagat gatcagggtc gtggcgcttc
     3361 accgcggcgt tcacgatttc gaggtatcgc gtgaacgcgg catgcacgag cgctttgcgt
     3421 atcgcgggcg tatcgccttt cgcgagttcg ctcttgaaac gctgctgcat cgaactggcg
     3481 gggccggcca gcacgagatc gacgagctgg ctttcgcgtg cgggccatgg aggctcgttg
     3541 ccgatgaaat agccgatcat ccacgggtcg gatttgcgcg gcgcaagctg ctgcgccgcc
     3601 tcggcgtcga cgcgcttcgc aaaggcttcg gagtacacgt cgggcagtcc catgatcgcg
     3661 ccctggctct gctgccagcc gcgaagcggg aaaacatagg gctgtcgcag cgccgcgccg
     3721 cgcggctgcg aatcgttgag cgaggggccg taagcggtgt tcagtcccca cgcccgcatg
     3781 cgtcgcgagg tcagctgtgc gctcgggccc tgccattcgc tcccgaagcg cttctcgaga
     3841 ttggcggcgt agaacgacac cggatcgcgc agtgggtcca gctcggttcc cggcgcggga
     3901 aactgcgcgg aggaggggat gctggcaaac aacttcgatc ggccgacgat ctgcgtgcgc
     3961 ggcggttcgg cgccggcgcc gttcacgccg gtggaataga agcggcaccc ttcgggatcc
     4021 acgaaccacc agcggtcgtt gatcttctcc acgcggaaga agcccgtgga tttgcggcgc
     4081 ccggccgggt atccgccgta gcggcattcc tggatcttgc ccttcggtac gagcatgcgg
     4141 ttttccgccg accattcgcg aacgagatcg ggataggagc gcgccttgcc gggccagtcg
     4201 gcatgcacgt attggccgaa gtcatcgacc agcggggcgc cgtcgtcgag cacggcgtcg
     4261 cccgggtccg tcttcgccag gctgatcttg cggatctcga ccgtcgcagc gcgcgccggg
     4321 tatctcatgg tcaggctgat ggcgcgcacg tgcagcacgg gggcgtgtcc gccggcttcg
     4381 atgttgatcc agtagctgtt gcgcggctgg ttgaccgtcg acgccagctc gtctgcatcg
     4441 ccgagtccct cgcgaaaata gcgcagcggt atcgaggcgc gtacccagac tcccgggaag
     4501 gggtgaatcc gcttggatac gttgccctcg tcgctgatga gtccgagttc gaatcgctgg
     4561 ctggtcgaag agcggaactc gaccaccagg aattcagcgt cggtccagtc gatgggtagc
     4621 tgggggtcga agtcagccag gggccactgg gtgctcgaga cgtcacgggc gaacgatgct
     4681 ttttgcgggg cggcctgagc cgtgatgccc t
//
LOCUS       DATDEU010000483         5049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_820945, whole genome shotgun sequence.
ACCESSION   DATDEU010000483 DATDEU010000000
VERSION     DATDEU010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820945"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..900)
                     /gene="atpB"
                     /locus_tag="VIV63_11505"
     CDS             complement(70..900)
                     /gene="atpB"
                     /locus_tag="VIV63_11505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY6125266.1"
                     /translation="MAAAEHGAEHAPSGSEYIVHHLGNLQYGEGFWTVNVDSIFFSVL
                     LGLLFVVSFWLAARKATASDVPRGFQSFVETVVEFVDSQVRDTFHGTSKIIAPLALTI
                     FCWILLMNFMDAVPVDLFPLMGQKVGLEHLKVVPTTDLQITLGMSITVFLIVMFYSFK
                     IKGFRGFTWELLTHPFGKWMMPFNLLLNFIEHLARPVSLGLRLYGNLYAGEMIFLLLA
                     VLGGSFAITSAAGIGGAIGQLVLGVVWACFHILVIPLQAFIFMVLTIVYLAQAHETHH
                     "
     gene            complement(913..1278)
                     /locus_tag="VIV63_11510"
     CDS             complement(913..1278)
                     /locus_tag="VIV63_11510"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY6125267.1"
                     /translation="MLGQVVVTVVAAAICFAVWGRISGLSALVGGGISSAASAVLALI
                     GFAAPAGAAAERVARSFYVGEAAKLAVTVTLFVVVFLMMKVSFVALFGTYIATLFVYW
                     IALANALPPLAGTASVAKR"
     gene            complement(1515..2279)
                     /locus_tag="VIV63_11515"
     CDS             complement(1515..2279)
                     /locus_tag="VIV63_11515"
                     /inference="COORDINATES: protein motif:HMM:NF039702.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HEY6125268.1"
                     /translation="MSSTASWRTFASTLALLGVLSAGCSERAVPPPPAVETPPPVPPS
                     DPPLLFSDDFDSDLSQWYIESEKPGTIAANNGMLDIDVPAGVTVWFKPRLEGPVSIEF
                     DATAVAAGGPNDQVSDLNVFWMANNVDAIVPVYARKRSGKFEEYNALLTYYVGLGGNR
                     NTTTRFRRYIGDPQLRPLRPGYDLAGEPYMLLANLPQTIRLVANGKVIEYWRDGTRIF
                     HLEDAEPYENGWFAFRTTYSHLRIDRFRVRRIAPAT"
     gene            complement(2245..3177)
                     /locus_tag="VIV63_11520"
     CDS             complement(2245..3177)
                     /locus_tag="VIV63_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY6125269.1"
                     /translation="MTTKKPVMGRGLEALLGQMSKRPDPAPGTADASAPPPPAKLPGD
                     ELANLPLDLLQRGKYQPRVDMRQESLEELAASIKSQGVIQPIVVRAVDGAAPGASQRY
                     EIIAGERRWRAAQIAGLATVPAVIRKVPDEAAIAMALIENIQREDLNPLEEARALERL
                     ISEFGITHQQAADAVGRSRAAVSNLLRLLELAPEITAFVERRELEMGHARALLALTQR
                     RQQVEVGTLVAKRGLSVRDTEGMVRGLLAKASGGSAPKDSKALDPNVQRLQDELTEKL
                     GATVQIQHTGSGKGKVVVSYHSLDELDGILAHIR"
     gene            complement(3266..4105)
                     /locus_tag="VIV63_11525"
     CDS             complement(3266..4105)
                     /locus_tag="VIV63_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY6125270.1"
                     /translation="MKRIIAVANQKGGVGKTTTAVNLAASFAATRRRVLLIDIDPQGN
                     ATMGCGVEKSQITRGTGDVLLGEADAASVIINLESTPGLALMPSNQDLTAAEVRLLTT
                     MAGRELKLRNALKPVYDQYEVILIDCPPALNMLTLNALVAAQSVLIPMQCEYYALEGL
                     SALVGTIEQIKGAVNPNLEIEGILRTMFDPRNNLANEVSAQLIAHFGDKVFRTIVPRN
                     VRLAEAPSFGKPALQHDKDSRGALAYLALAGEMLRKEDDAANDAEVVPQAEAAADGEH
                     PLN"
     gene            complement(4135..4794)
                     /gene="rsmG"
                     /locus_tag="VIV63_11530"
     CDS             complement(4135..4794)
                     /gene="rsmG"
                     /locus_tag="VIV63_11530"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY6125271.1"
                     /translation="MTDANNFDEAGWKAKLAAGAAELKAPLDDAQLDKLWRLGVLLRE
                     RNEHVNLTSIVSPEGILTLHMLDSLSIVPDLLEAATVLDVGTGGGFPGLPLAVACPQR
                     EFMLIDGTQKKIRFVNEAIEMLAIPNAKAVAVRAEDLDEPKYDAVIVRAVGSLADVLH
                     NAGHLVAPEGRLIAMKGRHPAEELRAVPRGWRAKVSVLRVPGLDAERHVVALTRQKRP
                     G"
     gene            complement(4796..>5049)
                     /gene="prpD"
                     /locus_tag="VIV63_11535"
                     /gene_synonym="mmgE"
     CDS             complement(4796..>5049)
                     /gene="prpD"
                     /locus_tag="VIV63_11535"
                     /gene_synonym="mmgE"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="functions in propionate metabolism; involved in
                     isomerization of (2S,3S)-methylcitrate to
                     (2R,3S)-methylisocitrate; also encodes minor aconitase or
                     dehydratase activity; aconitase C; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HEY6125272.1"
                     /translation="RHIGNALHIFFSDGSPKLELRIDFPVGHRKRRAEGMPLLVQKFE
                     SSVAAHFPAKQTESLKAVFARQDLDDMPVNEFMAAMVAN"
BASE COUNT          906 a         1554 c         1668 g          921 t
ORIGIN      
        1 gatttgagca attgcagcga tgttttccat ttttcctcca aacaaaaact tgtactaact
       61 accaaacgat cagtgatgag tctcgtgcgc ctgcgccagg tacacgatgg tcagcaccat
      121 gaatatgaat gcctgcagcg ggattaccaa gatgtggaag caggcccaca ccacgcccag
      181 cacgagctgg ccgatggcgc cgccgatacc ggcggccgaa gtgatggcga agctgccgcc
      241 cagcacggcg agcagcagga agatcatttc gccggcgtaa aggtttccgt acagacgcag
      301 gccgagcgag accgggcgtg cgaggtgctc gatgaagttc agcaggaggt tgaacggcat
      361 catccacttg ccgaagggat gcgtgagcag ttcccaggtg aagccgcgga aacctttgat
      421 cttgaagctg tagaacatga cgatcaggaa caccgtgatc gacatgccca gcgtgatctg
      481 gaggtcggtc gtcgggacca ccttcagatg ctcgagcccg actttctgac ccatcagcgg
      541 gaacaggtcc acgggcacgg cgtccatgaa attcatcagc aggatccagc agaagatcgt
      601 gagcgcgagc ggcgcgatga tcttgctggt gccgtggaag gtgtcgcgca cctggctgtc
      661 gacgaactcg acgacagtct cgacgaagct ctggaatccg cgcggaacgt cggacgccgt
      721 ggccttgcga gccgccaacc agaacgacac tacgaacagc aggcccagca gcacggagaa
      781 gaagatgctg tcgacgttca cggtccagaa tccttcgccg tattgaaggt tgcccaggtg
      841 gtgaacgatg tactcgctcc cggagggggc atgttcggcg ccgtgttcag ctgctgccat
      901 cgagtcttct tcctaacgct ttgcgacgct cgcggtcccg gccagaggcg gcagggcatt
      961 cgcgagagcg atccaataga cgaacaatgt ggcgatgtaa gtaccgaata gcgccacaaa
     1021 tgaaactttc atcatcaaga agaccaccac gaacagcgtg accgtcacgg ccagtttcgc
     1081 ggcctcaccc acgtagaagg agcgcgccac acgctcggcg gcagcgcctg ccggagccgc
     1141 gaaaccgatg agcgcaagca cggcgctggc ggcactcgag atgcctccgc ccacgagcgc
     1201 gctcaaaccg gagatacggc cccacaccgc gaagcaaatt gccgcggcca caaccgtaac
     1261 gacgacctga ccgagcacta cgcttccggc cagtcgcctc gcttggggca actcaagggc
     1321 gaccacgaca ctcctactgc aagtcgacaa tccgcgaaca aaacttcacg cccgcgagag
     1381 acgcaggctg attcggcaat gttcgaaaat tgtttggctt ggcaacgact tacgtgtgcc
     1441 ttggccaggg gcgcgcatta tatacgccga ccccccgccc ttcaacagaa aaccccgatt
     1501 tttccagggg ttttttatgt cgcaggcgcg atgcggcgca cccgaaaccg gtcgatgcgc
     1561 agatggctgt acgtggtgcg gaaagcgaac catccgttct cgtacggctc ggcatcttcg
     1621 agatggaata tccgggttcc gtcgcgccag tattcgatga ccttgccatt ggctacgagc
     1681 cggatcgtct gcggcaggtt ggccaagagc atgtacggct cgccggcaag atcgtagccc
     1741 ggacgcagag gccgcaactg cggatcgccg atgtagcgtc ggaaccgcgt cgtcgtattg
     1801 cggttgccgc cgagtccgac gtagtaggtc agcagcgcgt tgtattcctc gaacttccca
     1861 ctgcgcttgc gcgcgtacac cggcacgatc gcatcgacgt tattcgccat ccagaacacg
     1921 ttgaggtcgc tgacctggtc gttcggtcca cccgcggcca ccgcggtcgc atcgaactcg
     1981 atcgagaccg gtccctcgag acgcggtttg aaccacacgg tcacgccggc gggaacgtcg
     2041 atgtcgagca tgccgttgtt cgcggcgatg gtgcccggct tctccgactc gatgtaccac
     2101 tgcgagaggt cagagtcgaa gtcgtcgctg aacagcaggg gcggatcgga aggtggaacg
     2161 ggcggcggtg tttcgacggc aggcggtgga ggcacggcgc gctcgctgca cccggccgac
     2221 aacacaccga gcagcgccag cgtgctagcg aatgtgcgcc aggatgccgt cgagctcatc
     2281 taatgagtgg tagctgacga ccactttgcc cttgccggac ccggtgtgct ggatctgcac
     2341 cgtcgcgccg agtttttccg tgagctcgtc ctgcaggcgc tgcacattcg gatcgagcgc
     2401 cttgctgtct ttcggcgcgc tgccgcccga ggccttcgcg agcaggccgc gcaccatgcc
     2461 ttcggtgtcg cgaacggaaa gaccgcgctt ggcgaccagc gtgcccacct cgacctgttg
     2521 ccggcgctgg gtcagcgcca gcagcgcgcg cgcgtggccc atctcgagct cgcggcgttc
     2581 gacgaaggcc gtgatttccg gagcgagctc gaggagtcgc agcaggttgg aaacggcggc
     2641 gcgtgatcgg cccaccgcat ccgcggcctg ctggtgtgtg atgccgaact cgctgatgag
     2701 ccgctcgagc gcgcgggctt cctcgagcgg attcaggtct tcgcgctgga tgttctcgat
     2761 gagcgccatg gcgatggcgg cttcgtcggg caccttgcgg atgacggcgg gaacggtcgc
     2821 aaggccggcg atctgcgccg cgcgccagcg gcgttcgccg gcgatgattt cgtagcgttg
     2881 tgatgcgccg ggcgcggcgc catcgacggc gcgcacgacg atgggctgga tgactccctg
     2941 cgatttgatg gacgccgcga gttcttcgag cgattcctgg cgcatgtcga cgcgcggctg
     3001 gtatttgcct cgctgcagca ggtcgagcgg gagattggcg agttcgtcac cggggagctt
     3061 ggctggcggt ggcggcgcgg acgcgtcagc agtgccgggc gcgggatccg gacgcttgga
     3121 catctggccg agcagggctt cgaggccgcg tcccatcacc ggtttcttgg tggtcatggg
     3181 ggcggatgat agcgaggtgg cggaagaatg gggtcaggca ccaatttgcg gacgcaaatg
     3241 gaagccagac cccggcagga ccccgctagt tcagcgggtg ctcgccgtcg gcggcggctt
     3301 cggcctgtgg cacgacttcg gcgtcgttgg cggcatcgtc ttccttgcgg agcatttcgc
     3361 cggcgagcgc gagataggcc agcgcgccgc gcgaatcctt gtcgtgctgc agggcgggct
     3421 tgccgaaaga cggggcctcg gcgagacgca cgttgcgcgg cacgatggtg cggaacacct
     3481 tgtcgccgaa gtgcgcgatg agctgggccg acacttcgtt ggcgaggttg tttcgcggat
     3541 cgaacatcgt gcgcaggatg ccctcgatct cgaggttggg gttcaccgcg cccttgatct
     3601 gctcgatggt tccgaccagc gcggacaacc cttcgagcgc gtagtactcg cactgcatgg
     3661 ggatgagcac gctctgcgcc gcgaccagcg cgttcagcgt gagcatgttg agcgccggcg
     3721 ggcaatcgat gaggatgact tcgtactggt cgtagacggg cttcagcgcg ttgcgcagct
     3781 tgagctcgcg gcccgccatg gtagttagca gacggacttc ggcggcggtg agatcctggt
     3841 tcgacggcat gagcgcgagg ccgggcgtcg attcgaggtt gatgatgacc gacgcggcgt
     3901 cggcctcgcc taacaacacg tcgccggtgc cgcgcgtgat ctgcgacttt tcaacaccgc
     3961 agcccatggt ggcgttgccc tgcgggtcga tgtcgatgag cagcacgcgg cggcgtgtcg
     4021 cggcgaacga cgccgcgaga ttcaccgcgg tcgtggtctt gcccactccg cccttctgat
     4081 tcgcgaccgc gatgattcgc ttcatggcgg catcttactc cgcgactaca gaacttatcc
     4141 gggccgtttt tgacgcgtga gagcgacaac gtggcgttcg gcgtcgagtc ccgggacacg
     4201 cagcacggaa acttttgcac gccagccgcg cggcacggca cgcagctctt cggcgggatg
     4261 acggcccttc atcgcgatca gccggccctc gggcgccacc aggtgcccgg cgttatgtag
     4321 tacgtcggca agactgccga ccgcgcgcac gatgaccgcg tcgtacttcg gctcgtcgag
     4381 atcttccgcg cgtaccgcaa ccgccttggc gttcgggatg gccagcatct cgatggcttc
     4441 gttcacgaag cggatcttct tctgcgtgcc gtcgatgagc atgaattcgc gctgcggaca
     4501 ggcgaccgcg agcggcaggc ccgggaagcc gccgcccgtg ccgacgtcga gaaccgtcgc
     4561 cgcttcgagc aggtcgggca cgatcgacag gctgtcgagc atgtgcagcg tgagaatgcc
     4621 ttcgggcgag acgatcgacg tgaggttcac gtgttcgttg cgctcgcgca gcagcacgcc
     4681 caggcgccac agcttgtcga gctgcgcgtc atcgagcggc gctttgagct cggcggcgcc
     4741 cgcagccagt ttcgctttcc agcccgcttc gtcgaagttg ttagcgtccg tcacgtcagt
     4801 tcgcgaccat cgcggccatg aattcattca ctggcatgtc gtcgagatcc tggcgcgcga
     4861 acacggcctt gaggctctcg gtttgtttgg ccggaaagtg cgcggccacc gacgattcga
     4921 acttctgcac cagcagtggc atgccctcgg cgcgtcgctt gcgatgcccc acggggaagt
     4981 cgatgcgcag ctccagtttg ggactgccgt cgctgaagaa gatgtgcagc gcgttgccga
     5041 tgtggcgct
//
LOCUS       DATDEU010000484         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_381195, whole genome shotgun sequence.
ACCESSION   DATDEU010000484 DATDEU010000000
VERSION     DATDEU010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381195"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1203
                     /locus_tag="VIV63_11540"
     CDS             10..1203
                     /locus_tag="VIV63_11540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6125273.1"
                     /translation="MAELAQQELRGGLAFDQAQLHAQRRVRAFLRRTVEGCQRLAELD
                     HHYNLRASVQHGAVMAAVTEADMAGLRADYQFMDDSNLRALMPMTDKRRARFFETLSA
                     AQVQRRYGTAEGFYAGGVVDRFGGSFRPRKFLIGLARALQKRGVRFFQGTEAQALDFS
                     DGQVSVFCGNGANIRANTLFMANAYARHINGDVLERAIFEYDYVVAIELPDGAKTLSS
                     GTVLSDTREPCFYARRHGRRLYMGYEETPGTSPDITRNVARRTLAEGKRVFPGLAAVG
                     ERDIRSAWCGRVFYTLDDYPFVERRHGGRVITFAAPSDHGNSLALRIGQIVGNTVAPA
                     TLRPRSDDDIRQRRRNAQQLRLFESFPKGLRLRPGKRYQEAAFRETEPAAPEHLPPAC
                     EPDLP"
     gene            complement(1319..1846)
                     /locus_tag="VIV63_11545"
     CDS             complement(1319..1846)
                     /locus_tag="VIV63_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY6125274.1"
                     /translation="MNASSTRRSESTGYVHAVSRMVVDAAGMTTIRVLMNADTRRLVR
                     RTCSPASRRKQQRITHMSTRRVLVTSAVLALLSVPAFAQGVQPTAPAHPAPAAAAPKP
                     TTTGAATHPVAAKLNLNTATVAELDKAPQIGPTRSKAIVEARAKEKFKNWDDFVARKV
                     VPADAAAAMKDVVSF"
     gene            complement(2009..>2782)
                     /locus_tag="VIV63_11550"
     CDS             complement(2009..>2782)
                     /locus_tag="VIV63_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125275.1"
                     /translation="LRRAHPLALDEFLFLRDAATVTAKITLPAPSTMHFLRFADFADR
                     SAYADVDAFFADLIAVFAQEIAGLVQAGCRYVQLDEIAIALLCDPSIRERVAAAGGDP
                     GALVDRYIDAINGAVAACPDEVVVGVHMCRGNFRGHYLADGGYDAVAERFFAGTDANH
                     FLLEYDTPRAGDFAPLRFVPKGKGVVLGLVSSKTPALESLDSLQRRAEEAASHIDLDR
                     LAISPQCGFASSAAGNPLSEADERAKLQRVVEAARAIWR"
BASE COUNT          486 a          892 c          922 g          482 t
ORIGIN      
        1 gacgtcatgt tggcggaact ggcccaacag gagctgcggg gtggcctcgc cttcgatcag
       61 gcgcagttgc acgcccaacg ccgggtaagg gcattcctgc gccgcacggt cgagggatgc
      121 cagcgtctcg ccgaactcga ccatcactac aatctgcgcg cgtcggtgca gcacggcgcg
      181 gtcatggcgg cggtcaccga ggcggatatg gcgggtctgc gcgccgacta ccagttcatg
      241 gacgacagca atctgcgcgc gctcatgccc atgacagaca agcgccgcgc gcgattcttc
      301 gaaaccctct ccgccgcaca ggttcagcgg cggtacggca ccgcggaggg tttctacgcc
      361 ggcggcgtgg tcgaccgctt cggcggcagt tttcgtccgc gcaagtttct gatcggactg
      421 gcgcgcgcgc tgcagaagcg cggcgtgcgc ttctttcagg gcacggaggc gcaggcgctc
      481 gatttttccg acggccaggt cagcgtgttc tgcggcaacg gagccaacat ccgcgccaac
      541 acgctgttca tggcgaatgc ctatgcgcgg cacatcaacg gcgacgtgct cgagcgtgcg
      601 atcttcgagt acgattacgt cgtggcgatc gagcttccgg atggcgcgaa gacgctctcc
      661 tccggcaccg tactctccga tacgcgcgag ccgtgctttt acgcgcgccg ccatggccgg
      721 cggctctata tgggctacga ggaaacaccg ggaacctcgc ccgacatcac gcgcaacgtc
      781 gcccgccgaa cgctggccga gggcaagcgg gtgtttccgg gactcgctgc cgtcggcgag
      841 cgcgatatca ggagcgcatg gtgcgggcgg gtgttctaca ccctggacga ctatcccttc
      901 gtcgaacgcc ggcatggcgg ccgcgtcatt accttcgcgg cgccgtccga tcatggcaat
      961 tcgctggcgc tgcgcatcgg acaaatcgtg ggcaacactg ttgcccctgc aacgctccgg
     1021 ccgaggagcg acgatgacat ccggcagcgc cgccgcaatg ctcagcagtt gcggctgttc
     1081 gaaagcttcc cgaaaggact gcgcctgcgg ccgggcaagc gttatcagga agctgcattc
     1141 cgtgagactg aaccagccgc tccggagcat ctgccgccgg cttgcgagcc ggacttgccg
     1201 tgaggcgcgc gcaactcttc gaggcgatga cgtgggcgcc gggaagctcg aggcgcgcca
     1261 ttgcgcctcg agcacctttc gatcaagcgg ccgaaagtct gcagctttcg ggatgagatt
     1321 agaagctgac cacgtccttc atcgccgctg cggcatcggc cggaacgact ttgcgcgcca
     1381 cgaagtcatc ccaattcttg aacttctcct tggcgcgggc ctcgacgatc gccttggaac
     1441 gcgtcggccc gatctgcggg gccttgtcca gttcggccac ggttgcggtg ttgaggttga
     1501 gcttggccgc gacgggatgg gttgcggcgc cggtggtcgt gggtttcggc gcagccgccg
     1561 caggcgcggg atgagcgggt gccgtcggct gcaccccctg cgcgaaggcg ggaacggaca
     1621 ggagtgccag tacggccgag gtgacgagaa cgcgtcgagt cgacatgtgg gtaatcctct
     1681 gttgtttcct ccggcttgcg ggcgaacatg ttctgcgcac gagccgacgg gtgtctgcat
     1741 tcatcagtac ccggatggtc gtcatgcctg ctgcatccac caccatccga ctgacggcat
     1801 ggacatagcc ggtcgattcg gaacggcgcg tggacgacgc gttcatccgc tattcacgcg
     1861 gccaacaaag cgtcgtcaca aagctgacgg aatctcacaa ccgagttgaa gacgtgtcga
     1921 ctgagaccgg cagaaagatt ctggaggttc tcgagatcat tctcgcgaac actccgcaga
     1981 cgaaggttgg aaacgtcggc tcccggcctt agcgccagat cgcccgcgcc gcctccacaa
     2041 cccgctgcag cttcgcacgc tcatcggcct cgctcagcgg attgcccgca gcgctgctcg
     2101 cgaagccgca ttgcgggctg atcgcgagcc ggtcgagatc gatatggctt gcggcttctt
     2161 ccgcgcggcg ttgcaagctg tccaggcttt cgagcgcggg agtcttgctg ctgaccaggc
     2221 ccagcaccac gcccttgcct ttcggcacga agcgcagcgg cgcgaaatcg cctgcccggg
     2281 gcgtgtcgta ttcgagcagg aagtgattgg cgtcggtacc ggcgaagaag cgttccgcca
     2341 ccgcgtcgta cccaccgtcg gccagatagt ggccgcgaaa gttgccgcga cacatgtgca
     2401 cgccgaccac aacctcatcc ggacacgccg cgactgcgcc gttgatggca tcgatgtagc
     2461 gatcgaccag tgcgccgggg tcgccgcccg cggctgcgac ccgctcgcgg attgagggat
     2521 cgcacaggag cgcaatggcg atctcgtcga gttgcacgta ccgacatcct gcctgcacca
     2581 ggccggcgat ctcctgcgca aagaccgcga tgagatcggc gaagaaggca tcgacatcgg
     2641 cataggcgct gcggtcggcg aagtcggcaa agcgcagaaa atgcatggtc gagggcgccg
     2701 gcagcgtgat cttggccgtg accgtggcgg catcgcgcag gaagaggaat tcatcgagcg
     2761 cgagcggatg cgcgcgtcgc ag
//
LOCUS       DATDEU010000485         2088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_381625, whole genome shotgun sequence.
ACCESSION   DATDEU010000485 DATDEU010000000
VERSION     DATDEU010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381625"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VIV63_11555"
     CDS             <1..837
                     /locus_tag="VIV63_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6125276.1"
                     /translation="PLLDLARELQKSRKPDLRVAPGAPELREAEHAISQADVPGDAEC
                     ARSIGAMRFAGLHIDLAAARTGQGDFTGAARSYRSAHACRPRDAQVLSALAGVLFDAR
                     DYAGARDAINAALAIEPRAVSANRLAGNIDFVEERWADATARFRYVAASDPDRVQAAY
                     GQLMFWLAQMRAGVTRPEYVDRTPGEGWPQPLVLYLQGQYTEAELTAPIREGDQEDNP
                     QPNTSTDERLCEALFYVGEAYWARGESDVARDYFAALVNIKVIYFLEHGLALAEIAKL
                     RP"
     gene            complement(843..1205)
                     /locus_tag="VIV63_11560"
     CDS             complement(843..1205)
                     /locus_tag="VIV63_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6125277.1"
                     /translation="MPVTGIGGLFFRSRDPEKLMAWYLEHLGVGANMAAGQYVWDQAA
                     GPTVFAPFKADTDYFAKDKQWMLNLRVTQLDELLASLGKAGIEITTKAEWNTPETGRF
                     ARIHDPEGNPIELWEPPA"
     gene            complement(1221..>2088)
                     /locus_tag="VIV63_11565"
     CDS             complement(1221..>2088)
                     /locus_tag="VIV63_11565"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY6125278.1"
                     /translation="CDAGSLCLKSGNAAILRGGSESAHSSRAIHACLVEGLDAAGLPP
                     ASIQLVPTTDRAAVGYLLSSMTDYIDVVVPRGGKSLVARVQQEARVPVIGHLEGVCHV
                     YVDRDADLAMARAIVVNAKLRRTGVCGSAETLLVDAACAATHLAPVVRELLDAGCEVR
                     GDAAVQSVDPRVKPATEQDWYTEYLDAIIAARVVDGVDAAIEHIGKYGSSHTESIVTA
                     NAATAQRFLERVDSAIVLHNASTQFADGGEFGMGAEIGISTDRFHARGPVGVEQLCSY
                     KYVVRGTGQIRP"
BASE COUNT          336 a          718 c          692 g          342 t
ORIGIN      
        1 cccctgctcg atctcgcgcg cgaactgcag aagtcgcgca aacccgacct gcgcgtcgcg
       61 ccgggcgcgc ccgaattgcg cgaggcggaa cacgcgattt cccaggcgga cgtacccgga
      121 gacgccgaat gcgcgcgcag catcggcgcg atgcggttcg cgggattgca tatcgacctc
      181 gccgcggcgc gcaccgggca aggcgacttc acgggcgccg cccggtctta tcgcagtgcg
      241 catgcgtgcc ggccccggga cgcacaggtc ctctccgcgc tcgccggcgt cctgttcgac
      301 gcgcgcgact atgccggcgc gcgcgacgcc atcaacgccg cgctcgcgat cgagccacgg
      361 gccgtgagcg cgaaccgcct ggcgggaaac atcgacttcg tcgaggagcg ctgggccgac
      421 gcgaccgcgc gttttcgcta cgtcgcggcg agcgatcccg atcgtgtgca ggccgcctac
      481 ggtcagctca tgttctggct ggcgcagatg cgcgccggtg tgacccggcc cgaatacgtc
      541 gatcgcacgc ccggcgaggg ctggccgcaa ccgctggtgt tgtacctgca ggggcaatac
      601 accgaggccg aactcaccgc gccgatccgc gagggcgacc aggaagataa tccgcagccc
      661 aacaccagca ccgacgaacg actgtgcgaa gcgttgttct atgtgggcga agcctactgg
      721 gcacgcggag agtcagacgt ggcgcgcgat tatttcgcgg cgctggtgaa catcaaggtc
      781 atctacttcc tcgagcacgg tctcgcgctc gcggagatcg ccaagctgcg gccctgatca
      841 gctcaggccg gcggttccca caattcgatg gggttgcctt cgggatcgtg gatacgcgcg
      901 aaccgcccgg tctcaggcgt gttccactcg gccttggtcg tgatctcgat gcccgctttg
      961 cccaggctcg ccagcagttc gtcgagctgg gtgacccgca ggttgagcat ccattgcttg
     1021 tccttcgcga agtaatccgt gtccgccttg aacggcgcga acacggtggg gcccgccgcc
     1081 tggtcccaca cgtactggcc agcggccatg ttggcgccga cgcccaggtg ctcgagatac
     1141 caggccatca acttctctgg atcgcgactg cggaagaaca aaccgccgat tccggtgact
     1201 ggcatgtctg cgactccctt ttacggcctg atctggcccg tgccccgcac gacgtacttg
     1261 tagctgcata gctgctccac gcccaccggc ccgcgcgcgt ggaaccggtc ggtggagatg
     1321 ccgatctcgg cgcccatgcc gaactcgccg ccatcggcga actgcgtgga agcgttgtgc
     1381 aacacgatgg cgctgtcgac gcgctcgagg aaacgctggg cggtcgcggc gttggcggtg
     1441 acgatgctct cggtgtgcga cgagccgtac ttgccgatgt gttcgatggc cgcatcgacg
     1501 ccgtcgacca cgcgcgccgc gatgatggcg tcgagatatt cggtgtacca gtcctgttcc
     1561 gtggcgggct tcacgcgcgg atccacgctt tgcacggcgg cgtcgccgcg cacctcgcag
     1621 cccgcatcga gcagttccct gaccaccggc gccaggtgcg tggccgcgca ggcggcatcg
     1681 actaacaaag tctccgccga accgcacacg ccggtgcggc gcagcttggc gttcacgacg
     1741 atggcgcgcg ccatcgcgag atccgcatcc cggtcgacat acacatggca tacgccttcg
     1801 agatgtccga tgaccggcac acgcgcttcc tgctgaacgc gcgcgaccag gctcttgccg
     1861 ccgcgcggca ccaccacgtc gatgtaatcg gtcatcgacg acagcaggta gccgaccgcg
     1921 gcgcggtcgg tggtcggaac cagctgaatg ctcgcgggcg ggaggccggc ggcgtcgagt
     1981 ccttcgacca agcaggcgtg gatggcgcgg ctcgagtgcg cactttccga cccgccgcgc
     2041 aggatggccg cgttgccgga tttgaggcac aaactaccgg cgtcgcag
//
LOCUS       DATDEU010000486         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1258636, whole genome shotgun sequence.
ACCESSION   DATDEU010000486 DATDEU010000000
VERSION     DATDEU010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258636"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VIV63_11570"
     CDS             complement(<1..373)
                     /locus_tag="VIV63_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6125279.1"
                     /translation="MKGHTSQDPHLLRRLLRAKDRMDVASHEDWPVERLARVSRVSEA
                     HFARSFKDAFGLPPHRYLLTRRMERAKALLRDTDLSVTDIAFQTGWKSLGTFGRIFRD
                     VNGESPGELRAREKAAKHAFDR"
     gene            complement(401..1576)
                     /locus_tag="VIV63_11575"
     CDS             complement(401..1576)
                     /locus_tag="VIV63_11575"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY6125280.1"
                     /translation="MTRDAVIVATARTPIGRAYRGAFNHTPAPTLAAHALRAAVSRAN
                     VAPAEVEDCLLGCAMPQGSQHTLGRTAALRAGFPVEVPGMTLDRQCASGLMAIATAAK
                     QVIVDRMDIVIAGGVESISLVQTPALRIEQDPELLALQPDAPMPMIGTAEIVASRYGI
                     SRERCDAYALASQQRTAAAQAAGRFGGEIVSVSATMKVVDKNTGAESLRDITLDRDEG
                     MRPDTTLAGLAALKTVQSGGTVSAGNASQLSDGASACVLMEARLAERRGLAPLGRYVG
                     MAVTGTRPDEMGIGPVFAVPRLLERFGLKVDDIGLWELNEAFAVQVLYCRDRLGIPDE
                     RLNVDGGAISVGHPYGMSGARLVGHALIEGRRRGVRHVVVTMCVGGGMGAAGLFEVL"
     gene            complement(1573..>2177)
                     /locus_tag="VIV63_11580"
     CDS             complement(1573..>2177)
                     /locus_tag="VIV63_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6125281.1"
                     /translation="DKSFAKMSLDDFRLVLDVHLMGAVICTKAVWDRMREQKHGRIVM
                     TTSSSGLFGNFGQANYGAAKMALVGLMQTLGIEGEKYDIRVNCLAPSAATQMTQGVLS
                     EASLRAMNPALVSPALLPLVVDDAPTRAIVCAGAGHFARSYITLTQGQFIGSGDEAGV
                     RVIENWDAISERAGALVPAYGFLQVEREVASAAGATPATP"
BASE COUNT          373 a          742 c          692 g          370 t
ORIGIN      
        1 ctcgatcgaa cgcgtgcttc gcggctttct cgcgcgccct cagttctccg ggactctcgc
       61 cattcacgtc gcgaaagatt cgaccgaagg tccccaggct tttccagccc gtctggaacg
      121 caatgtcggt gaccgacaga tccgtgtccc gcagcagggc tttggcccgc tccatgcgtc
      181 gtgtgagcag ataacggtga ggcggcagtc cgaaagcgtc cttgaacgac cgcgcgaaat
      241 gcgcttcgga aacccggctc acgcgcgcaa gccgctcgac gggccagtct tcatgcgacg
      301 cgacgtccat gcggtctttg gcgcgcaaca ggcgacgcaa gagatgggga tcctggctcg
      361 tgtgcccttt catacgcggg cgatattgtg cctgagcgcg ctacagaacc tcgaacaggc
      421 cggcggcgcc catgccgccg cccacgcaca tggtcacgac cacgtggcgc acgccgcgcc
      481 gcctgccttc gatgagcgca tgccccacca gcctcgcgcc cgacatgcca tacggatgcc
      541 cgacggaaat ggcgccgccg tcgacgttca atcgctcgtc cggaattccc agccggtcgc
      601 ggcagtaaag cacctgaacg gcgaaagctt cgttcaattc ccacagcccg atgtcgtcca
      661 ctttgagtcc gaaccgctct aacaaccgtg gcaccgcgaa cacgggaccg atgcccatct
      721 cgtcgggacg agtgccggtc accgccatgc cgacatagcg cccgagaggc gcgagtccac
      781 gccgttccgc cagccgtgct tccatgagca cacaggccga ggcgccgtcc gagagctggc
      841 tggcgttgcc cgccgagacc gtgccgcccg actgcacggt cttgagcgcc gcgagtcccg
      901 cgagcgtggt gtcaggccgc atgccttcgt cgcgatcgag cgtgatgtca cgcaacgatt
      961 ccgcgccggt gttcttgtcc accactttca tggtggcgct cacggagacg atttcgccgc
     1021 cgaatcgacc ggccgcctgc gcggcggccg tgcgttgctg tgacgccaag gcatacgcgt
     1081 cgcagcgttc acgcgagatg ccataccgcg acgccacgat ctcggcggtg ccgatcatcg
     1141 gcatgggcgc atccggttgc agggccagta gttcgggatc ctgctcgata cgcaaagcgg
     1201 gcgtctgcac cagcgatatg gattcgacgc cgcccgcgat cacgatgtcc atgcgatcga
     1261 cgatcacctg ctttgcggcg gtggcgatcg ccatgagacc ggacgcgcat tggcgatcga
     1321 gcgtcatgcc cggcacttcc acagggaatc ccgcgcgcaa cgccgccgtg cggccgaggg
     1381 tgtgttgtga tccctgcggc atcgcgcagc ccagcaaaca atcttcgact tccgcgggcg
     1441 cgacattcgc gcgactgacc gcagcgcgca gcgcgtgtgc tgccagcgtg ggtgcgggtg
     1501 tgtgattgaa cgcgccgcga taggcgcggc cgatgggcgt tcgcgcggta gcgacgatca
     1561 ccgcatcgcg tgtcatggcg tcgcgggggt ggcgcctgcc gcgctggcga cttcgcgctc
     1621 cacttgcagg aaaccgtagg ccggcacgag cgcgccggcg cgttcggaga tggcgtccca
     1681 gttttcgatg acgcgcacgc cggcttcatc accgctgccg atgaactgtc cttgcgtcag
     1741 cgtgatgtag gaacgtgcga agtggcccgc accagcgcag acgatggcgc gtgtgggcgc
     1801 gtcatccacc acgaggggca gcaacgcggg actgaccagc gcgggattca tcgcacgcag
     1861 gctggcctcc gacaaaacgc cttgcgtcat ctgcgtcgcg gccgacggcg cgaggcaatt
     1921 cacgcggatg tcgtacttct cgccttcgat acccagcgtc tgcatgaggc ccaccagcgc
     1981 catcttcgcc gcaccgtagt tggcctggcc gaagttgccg aacaacccgg atgaagaagt
     2041 ggtcatgacg atgcggccgt gcttctgctc gcgcatgcgg tcccacacgg ccttggtgca
     2101 gatcaccgcg cccatgagat ggacgtcgag caccaggcga aaatcgtcga ggctcatctt
     2161 cgcgaaactc ttgtcac
//
LOCUS       DATDEU010000487         7303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1842267, whole genome shotgun sequence.
ACCESSION   DATDEU010000487 DATDEU010000000
VERSION     DATDEU010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842267"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VIV63_11585"
     CDS             <1..191
                     /locus_tag="VIV63_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125282.1"
                     /translation="DLEDWQAAQEIGALEEIEPPEDWTGSVDVDPEEDDARGTVGYDK
                     EDDRRQSAGKLEGLDLFE"
     gene            complement(202..585)
                     /locus_tag="VIV63_11590"
     CDS             complement(202..585)
                     /locus_tag="VIV63_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6125283.1"
                     /translation="MREIDTTRVLCSLTELDETGSRGFAVGEGDWPLRGFIVKTADGV
                     AAFVNYCPHAGHPLNFRPHKFLTPDRNLILCASHGALFARDNGLCIAGPCPGQSLTPV
                     PVEVVSGYVMLAEGVDVERLANAPL"
     gene            complement(606..1886)
                     /locus_tag="VIV63_11595"
     CDS             complement(606..1886)
                     /locus_tag="VIV63_11595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6125284.1"
                     /translation="MSAETPAAPAKPRIREVLTQPRMLAILLLGAASGLPNPLSEAVM
                     QAWLTDLGISNTKIGLLMYVALPYLLKPLWAPLLDRYTLPWLGRRRGWIVLIQILLAA
                     AIGSLALFGGAHQLTAIALVLLVVVFLSASQDIVIDAYRTDVARPAERGLAAAAANLG
                     YRAFSYGALAVALIVADNVSWRAAFLTLSVAMAFMAFATLWAPEPDDPRGKTLPTLAD
                     SILIPLRELFGVPGMLALVLLIVLYKIGDAFALKFFTAFMINTGFTKTEIGVVVKAVL
                     TTGSIVGALLGGIWMIKLGLRRAMMLFAIVQALTNLGYLLLHAVGKNYAVMVGAVALD
                     ALASGMGNIASVALMMALCDHRFSAFQYALLSMMALLPRYTLGGPAGYIADHGGWGAY
                     YWTSFALALPGIALVWIMRKRIEALDVPQEKTPA"
     gene            1940..2632
                     /gene="pyrE"
                     /locus_tag="VIV63_11600"
     CDS             1940..2632
                     /gene="pyrE"
                     /locus_tag="VIV63_11600"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY6125285.1"
                     /translation="MQEYQRTFIELAMSRTALRFGSFKLKSGRESPYFFNAGLFNDGE
                     AIAVLGQCYASALARSQLGFDMLFGPAYKGIPLATTTAVALAEHHHRSVPWAFNRKEA
                     KDHGEGGSIVGAPLKGRVVIVDDVITAGTAIRESVEIIRAAGATPVGVALALDRQERG
                     QGTLSAVQEVSAQYGLQCVSILTLADLIDTFAQGPGDAVRISGEQFAALQAYQRDWGV
                     GAVSPGASAGAE"
     gene            2638..3330
                     /locus_tag="VIV63_11605"
     CDS             2638..3330
                     /locus_tag="VIV63_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY6125286.1"
                     /translation="MRKATLFLATALSALMAVSAVGAEKKRAKSSSAKSVGGTTYKWV
                     DENGVTHYGDHVPTEYSQREQTVLNSQGLELQKRQAEMTPKEAAEYAAKQKEESRRKQ
                     HDMFLISTYPSVKEIENVRDVRLDQINGQIVAAEAYISSLTTRVEGLKTRALNFAPYN
                     TKPGARRMPDDLAEEMVRAMSELRTQNSALTTKKTEHQGVVAQFDADIKRFKELRTSA
                     AARVNQANTPKQ"
     gene            complement(3333..3818)
                     /gene="dut"
                     /locus_tag="VIV63_11610"
     CDS             complement(3333..3818)
                     /gene="dut"
                     /locus_tag="VIV63_11610"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY6125287.1"
                     /translation="MNAQVSHLSADRALKVRILDPRIGKDFPLPAYATSGSAGLDLRA
                     CLDAPVTLPPGKAELIPTGLAIHLDDPGLAAVILPRSGLGHKHGVVLGNLVGLIDSDY
                     QGQLMVSCWNRSATEFVVQPGERIAQMIIVPVVQVRLEVVDDFAASARGAGGFGHTGT
                     R"
     gene            complement(3815..5041)
                     /gene="coaBC"
                     /locus_tag="VIV63_11615"
     CDS             complement(3815..5041)
                     /gene="coaBC"
                     /locus_tag="VIV63_11615"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY6125288.1"
                     /translation="MHGKRVLLGVTGGIAAYKSPDLVRRLRELGAEVQVVLTSGAREF
                     VTPMTFQAVSGREVRSDLWDPEAEKAMSHIELARWADFVIIAPATADFIARLATGQAD
                     DLLTTLCLATSAPIALAPAMNRLMWANAATQANVATLKSRGVHVWGPGEGDQACGEVG
                     AGRMLEPLQLAGNVANSVAPTGPLAGKRLLLTAGPTRERIDPVRFISNRSSGKMGFAL
                     AAAAREAGADVTIVCGPVTLATPPGVKRIDVESAADMLNAVMKNIDGVDIFVATAAVA
                     DYRPANPVECKIKKTSESMDLKMERTVDILATVAARVGAARPFVVGFAAETDSVEQYA
                     RQKLLKKNLDMIAANEVGHDKVFEKDDNALLVLWRDGRRELPHAPKVTLARDLIALVA
                     ELYADRKAPRLTSVSA"
     gene            complement(5146..>7303)
                     /locus_tag="VIV63_11620"
     CDS             complement(5146..>7303)
                     /locus_tag="VIV63_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125289.1"
                     /translation="NPNAALWRDFPMGVAVDGDFAMVGAEYWDQSNPQAPQLHQTTFL
                     YRRSGTGWVLVRQLAETVVNCCVPRNLLSVAMSNGVAAASTTPMTIYELGSSGWTLAP
                     SSIPIGNNSGFDLEIDNGRIINNEGACSWDAGIIERSPDGVWRKAATLTGATRTCESS
                     YAGSVDISGDRAVVHQENGPEFPYGEQQTWIFRRQGAQGWAREGVAALPIIWPAEQRL
                     FSYNTHAAISGADVFVGSGIVNGIKLYRDVPGQGFQMVDRIRPADGAMGGGDSAQLKT
                     SGNYLLQKTVLFDGSYSPTVLNVYRRGADARYEHVAALVRRGATEWNGPGYEQSWSNV
                     AISGRTVLASGLNQVYHFELPATLAAPQPLQETFNTGVAANWATSAGSQFAVVRGDRS
                     RVLRQSQTAIDTRAIYEPANWTNQAIEVDVKANQFADDSAAISLITRWQGPDNFYEFV
                     RGPHRMEFRRMASGTLRTLWSVPVSATDFFPTGRNFRLRLESIGARHQVFVDGRVYFS
                     LFSTGPTRGRVGIATYRAGADFDNVQVTPTPLYSIYRNYLSTAEPWPLASTVGDWRME
                     EGYNSVYRLAQFSTAGETRVTMGTPTADQRVEARARVIEFASPTGTQRRWFGVVARYV
                     DEGNFYTLALRNSNTLVLSKRVNGQDTTLGSFAASVVPEQYYLIRLDATGNQLRAYLN
                     DRLLFEATDDAHAIGSTGLATFRAHAEFAYYLAYQP"
BASE COUNT         1346 a         2385 c         2365 g         1207 t
ORIGIN      
        1 tggatctcga agactggcag gcagcgcagg aaatcggcgc cctcgaagaa atcgaacctc
       61 ctgaagactg gaccggctcg gtggatgtag accccgagga agacgacgcg cgcggcaccg
      121 tcggttacga caaggaagac gaccgtcgcc agagcgccgg caagctggaa ggcctcgacc
      181 tgttcgagta ggccgctcta actacagcgg cgcgttggcc agccgctcca cgtccacgcc
      241 ttccgccagc atcacatacc cgctcaccac ttcgaccggc accggcgtca gcgactgacc
      301 cggacacggt ccggcgatgc acagtccgtt gtcgcgcgcg aacagcgcgc cgtgcgacgc
      361 gcacaggatg agattgcggt ccggggtcag gaacttgtgc ggccggaagt tgagcggatg
      421 tcccgcgtgc gggcagtagt tcacgaaagc ggcgacgcca tccgcggtct tcacgatgaa
      481 tccgcgcagc ggccagtcgc cctcgcccac cgcgaatccg cggcttccgg tttcgtcgag
      541 ctccgtcagg gaacagagca cgcgcgtcgt gtcgatctcg cgcatgcgcg cttaagggct
      601 ggcgctcatg ccggtgtttt ctcctgcggc acatcgagcg cttcgatccg tttgcgcatg
      661 atccagacga gcgcgattcc cggcagcgcc agtgcgaagc tggtccagta gtaagcgccc
      721 cagccaccat gatcggcgat gtacccggcc ggtccgccga gcgtgtaccg cggcaacagc
      781 gccatcatcg acaacaacgc gtactggaac gcgctgaagc ggtggtcgca cagcgccatc
      841 atgagcgcca ccgatgcgat gtttcccatg ccgctcgcga gtgcatcgag cgccaccgcg
      901 ccgaccatga ccgcgtagtt cttgcccacg gcgtgcaaca gcagatagcc gagattcgtc
      961 agcgcctgga cgatcgcgaa cagcatcatg gcgcggcgca gcccgagctt gatcatccag
     1021 atgccaccga gcagcgcacc gacgatcgag cccgtggtga gcacagcctt caccacgaca
     1081 ccaatctccg tcttggtgaa gccggtattg atcatgaacg cggtgaagaa tttcagcgcg
     1141 aacgcatcgc cgatcttgta tagaacgatg agcaggacga gcgcgagcat cccgggcaca
     1201 ccgaacaact ctcgcagcgg tatcagtatc gaatccgcca gtgtaggcag cgtcttgcca
     1261 cggggatcgt ccggctccgg agcccagaga gtcgcgaaag ccatgaacgc catcgcgacc
     1321 gacagcgtca ggaacgcggc gcgccagctc acgttgtcgg ccacgatgag cgcgacggcc
     1381 aacgccccgt aggaaaacgc gcgatagccg agattggcgg cagccgcggc cagtccgcgc
     1441 tccgcgggcc gcgcgacatc ggtgcggtac gcatcgatga cgatgtcctg cgacgccgac
     1501 aggaacacca ccacgagcaa cacgagcgca atcgcggtga gctggtgcgc gcctccgaac
     1561 aacgcgagcg agccgatcgc agccgcgagc aggatctgga tgagcacgat ccagccgcgc
     1621 cgccggccaa gccagggaag cgtgtagcga tccagcaacg gcgcccacaa tggtttcagc
     1681 aggtacggaa gcgcgacgta catcagcagg ccgattttcg tgttgctgat gccgagatcg
     1741 gtcagccatg cctgcatcac ggcttcagag agcgggttcg gcaatccgga ggcggcgcct
     1801 aacaacagga tcgcgagcat gcgcggctgc gtgagtactt cgcgaatgcg cggcttggcc
     1861 ggagccgccg gtgtctctgc gctcaagtct ggttgtccgt tatcatgcgc accgtcgcat
     1921 catacggaag actccgcccg tgcaggaata tcaacgaacc ttcatcgagc tcgccatgtc
     1981 gcgcaccgcg ctgcgcttcg gctcgttcaa gctcaagtcc ggccgcgaga gtccgtattt
     2041 tttcaacgcc ggcctgttca acgacggtga ggccatcgcc gtcctcggcc agtgttacgc
     2101 ctcggcgctt gcgcgctcgc agctcggctt cgacatgttg ttcggcccgg cgtacaaggg
     2161 catcccgctt gccaccacca ccgccgtggc gctcgccgag catcaccatc gcagcgtgcc
     2221 ctgggcattc aatcgcaagg aggccaagga tcacggcgag ggcggcagca tcgtgggcgc
     2281 gcctctcaag ggccgcgtcg tgatcgtcga tgacgtcatc accgccggca ccgcgatccg
     2341 cgagtcggtg gagatcattc gtgccgcggg cgccacgccg gtcggcgtcg ccctcgcgct
     2401 ggaccgccag gaacggggcc agggcaccct gtccgccgtg caggaagtgt cggcgcagta
     2461 tggattgcag tgcgtcagca tcctcacgct ggcggacctc atcgatacct tcgcccaggg
     2521 ccctggtgat gcagttcgca tttctggcga acagtttgcc gcattgcagg cttatcagcg
     2581 tgattggggc gtcggcgcgg tatcccccgg cgcgagtgct ggggcagaat agcgcgcatg
     2641 cgtaaggcca ctctcttcct ggcgacggcg ctttcagccc tgatggcggt ttccgccgtg
     2701 ggcgcggaaa agaagcgagc caagagctcg agcgccaaat cggtcggcgg cactacctac
     2761 aagtgggtcg acgagaacgg cgtgacgcat tacggcgacc acgtccccac tgaatattcc
     2821 cagcgcgaac agacggtcct caattcccag ggactcgagc tccagaagcg ccaggcggaa
     2881 atgacgccga aggaagctgc cgagtacgcg gcgaagcaga aggaagagtc gcggcgcaag
     2941 cagcacgaca tgttcctgat ctccacctac ccgtcggtga aggaaatcga gaacgtgcgc
     3001 gacgtgcgcc tcgaccagat caatggccag atcgtcgccg ccgaagcgta catctcgagc
     3061 ctcactaccc gcgtcgaagg gctcaagacc cgcgcgctca atttcgcgcc ttacaacacc
     3121 aagcccggcg cgcgccgcat gcccgacgat ctcgccgagg aaatggtccg cgccatgagt
     3181 gagctgcgca cgcagaattc agcgctgacg acgaagaaga ccgaacacca gggcgtggtg
     3241 gcgcagttcg acgccgacat caagcggttc aaggaattgc ggacgtctgc agccgcgcgc
     3301 gtcaaccagg cgaatacgcc gaagcaataa cttcagcgag tgcccgtgtg gccgaagcca
     3361 ccggcgccgc gtgccgacgc ggcaaaatca tccacgactt ccagccgtac ctgcaccacg
     3421 gggacgatga tcatctgcgc gatgcgctca cccggctgca cgacgaactc cgtggccgag
     3481 cgattccagc aggacaccat gagttgtccc tggtaatcgg aatcgatcag cccgacgaga
     3541 ttgcccagca ccacgccgtg tttatggccg agacccgagc gcggcaggat caccgcggca
     3601 agaccggggt catcgaggtg aatcgcgaga cccgtgggaa tgagttctgc cttgcccggc
     3661 ggcagcgtaa ccggagcatc gaggcaggcg cgaagatcca ggcccgcgga acccgacgta
     3721 gcgtacgccg gcagcggaaa atccttgccg atgcgcggat cgagaatgcg gactttgagc
     3781 gcacgatcgg cgctcaggtg cgacacctgc gcgttcatgc cgacacgctg gtgaggcgcg
     3841 gagccttgcg atctgcgtag agctcggcga ccagcgcgat gagatcgcgt gccagcgtga
     3901 ccttgggcgc gtgcggcagc tcgcggcgcc catcgcgcca caacaccagc aatgcgttgt
     3961 cgtctttctc gaacaccttg tcgtgtccga cttcgttcgc agcgatcatg tcgaggttct
     4021 tcttgaggag tttctggcgc gcgtattgtt ccacggaatc cgtctcggcc gcgaacccga
     4081 ccacgaacgg ccgcgccgct ccgacgcgcg ccgccacggt tgcgaggatg tcgaccgtgc
     4141 gctccatctt gagatccatc gactcggacg tcttcttgat cttgcactcg accggattcg
     4201 ccggccggta gtcggccacg gcagcggtgg cgacaaagat gtcgaccccg tcgatgttct
     4261 tcatcaccgc gttgagcatg tcggcggcgc tctccacgtc gatgcgcttc acgccgggcg
     4321 gagtcgcgag cgtgaccggc ccgcacacga tggtgacgtc agcccccgct tcacgcgcgg
     4381 ccgcggccag cgcgaacccc atttttccgg agctgcgatt gctgatgaag cgtaccgggt
     4441 cgatgcgttc gcgcgtggga cctgcggtga gcaacaggcg cttgcccgcg agcggacccg
     4501 tgggagcaac cgagttggcc acgttgccgg cgagttgcag cggctcgagc atgcgtccgg
     4561 ccccgacttc gccgcaggcc tggtcgcctt cgccgggacc ccagacatga acgccgcgcg
     4621 atttcagcgt ggcgacgttc gcctgcgtgg cggcgttcgc ccacatcagg cggttcatgg
     4681 ccggcgccag cgcgatcggc gcggaggtcg cgagacacaa cgtagttaga agatcgtcgg
     4741 cctggccggt ggcgaggcgg gcgatgaagt cggcggtggc gggcgcgatg atcacgaagt
     4801 cggcccagcg cgcgagctcg atgtgcgaca tcgccttctc ggcctcgggg tcccacagat
     4861 cgctgcgcac ttcgcggccc gacacggcct ggaaggtcat gggggttacg aactcgcgcg
     4921 cgcccgaggt gaggaccacc tgcacttcgg cgcccagttc acgcaggcga cggacgagat
     4981 ccgggctctt gtaagccgcg ataccgcctg tgacgccgag aaggacgcgt ttgccgtgca
     5041 tgggccggca ttatcgacag gcgcgtcggg gctttgccag ttcgctttgg ttcgggcgat
     5101 aggccctgcg gttatgcctc gcaaatcccg acatggatca gcaacttagg gttggtaagc
     5161 cagatagtag gcaaattcgg cgtgcgccct gaaggtcgcc agacccgtcg agccgatggc
     5221 atgcgcgtcg tcggtggctt cgaacagcag ccgatcgttc aggtacgcac gcaactggtt
     5281 gccagtggcg tcgaggcgga tcaggtagta ctgctcgggg accacgctcg cggcaaagct
     5341 gccgagcgtc gtgtcctgac cattcacgcg cttgctgagt acgagggtgt tcgaattgcg
     5401 aagcgccagg gtgtagaaat tgccttcatc gacgtagcgc gccaccacgc cgaaccatcg
     5461 ccgctgggta cccgtgggag acgcgaactc gatgacccgg gccctggcct ccacacgctg
     5521 atcggcagtc ggggtaccca tggtcacgcg cgtctcgccc gcggtggaaa actgcgcgag
     5581 ccggtacacg ctgttgtatc cctcttccat tcgccagtcc ccaaccgtac tggccagcgg
     5641 ccagggctcg gcggtactga ggtaattccg gtagatagag tagagcggcg tcggcgtgac
     5701 ctgcacattg tcgaaatcgg cgccggcccg gtacgtggcg atgccgacgc gccctcgcgt
     5761 cggcccggtg ctgaagaggg agaagtaaac ccggccatcg acgaagacct ggtggcgcgc
     5821 gccgatcgat tcgagccgca gccggaagtt gcgcccggtc gggaaaaaat ccgtcgccga
     5881 taccgggacc gaccacagcg tgcgcagtgt gccgctcgcc atgcgccgga actccatcct
     5941 gtgcggccca cggacgaact cataaaaatt gtcgggcccc tgccagcggg tgatcaggga
     6001 aatggcagcc gagtcgtccg cgaactgatt ggccttcaca tccacttcga tggcctggtt
     6061 cgtccagttc gcgggctcgt agatggcgcg ggtgtcgatc gcggtctgcg actggcgcag
     6121 cacgcgcgag cgatcacccc gcaccacggc gaactgactg ccggccgacg tcgcccagtt
     6181 ggcggcgaca ccggtattga acgtctcctg cagcggctgc ggcgccgcga gcgtcgcggg
     6241 caattcaaag tgatagacct ggttcaggcc actggcgaga accgtgcgtc cgctgatggc
     6301 gacgttgctc cacgattgct cgtagccagg cccgttccat tcggtggctc cgcggcgcac
     6361 cagcgccgca acgtgttcgt aacgcgcatc ggctccgcgc cggtaaacat tcaggaccgt
     6421 cggcgagtaa ctgccgtcga acagcacggt cttctgcagt agatagttgc cgctggtctt
     6481 gagctgcgcg gaatctccgc cgcccatggc gccgtccgcg ggcctgatgc gatccaccat
     6541 ctggaatccc tggcccggca cgtcgcggta gagcttgatg ccattgacga tgcccgaccc
     6601 gacgaagaca tcggcgccgc tgatagcggc atgggtgttg tatgaaaaga gacgttgctc
     6661 ggcgggccag atgatcggga gggccgcgac gccttccctc gcccagcctt gcgcaccctg
     6721 gcggcggaat atccaggtct gctgctcgcc gtaggggaat tccgggccgt tttcctggtg
     6781 cacgacggcc cggtccccgg agatatcgac cgaccccgcg tagctggatt cgcacgtccg
     6841 cgttgcaccg gtcagcgtgg cggccttgcg ccagacaccg tccggggatc gctcgatgat
     6901 gccggcatcc caggagcacg cgccttcgtt gttgatgatg cggccgttgt cgatctccag
     6961 atcgaacccc gagttgttgc cgatgggtat cgacgacggc gccagcgtcc agccggacga
     7021 cccgagctcg tagatcgtca tgggcgtcgt gctcgcggcg gcaacaccat tgctcatcgc
     7081 aacgctgagg aggttgcgcg ggacgcaaca gttcacgacg gtttccgcga gctggcgcac
     7141 cagcacccat cccgtgccgc tgcgccggta caggaacgtg gtctggtgga gctggggcgc
     7201 ttgaggattg gattggtccc agtactccgc acctaccatg gcgaagtcgc catcgaccgc
     7261 gacccccatc gggaaatccc tccagagggc ggcattcgga ttc
//
LOCUS       DATDEU010000488         4794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1842399, whole genome shotgun sequence.
ACCESSION   DATDEU010000488 DATDEU010000000
VERSION     DATDEU010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842399"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VIV63_11625"
     CDS             <1..790
                     /locus_tag="VIV63_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY6125290.1"
                     /translation="TLFLDEIGDMSLPMQVKLLRVLQERVYERVGSNVSSHCDVRIIA
                     ATHRNLEESIVRGNFRGDLFYRLNVFPIEMPTLATRIDDLPVLIEDFVSVAASRGRPR
                     LQLLPDTIDALSAYSWPGNIRELSNLIERLAILCPERPVGVGDLPARYRPADWTAPEL
                     VLTPTLEIPQLLASMSGDAEIEEDEVVPTSMWVGEQDAITQLPEQGVDLRAHLMVIER
                     SLINQALQRSGGTVAHAARLLSLRRTTLVEKLRKLGMAQTAEPA"
     gene            966..1934
                     /locus_tag="VIV63_11630"
     CDS             966..1934
                     /locus_tag="VIV63_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6125291.1"
                     /translation="MKLSDALEPVAVDSSSLLTMALARRVAVTDCSVLIVGESGTGKE
                     VLARLIHRASPRAKNNFVAVNCAAIPENMLEAMLFGYEKGSFTGAVNAHAGKFEQAQG
                     GTILLDEITEMPLALQAKLLRVLQEREVERLGGNTPVALDVRVIATTNRLLRDEVAAG
                     RFREDLYYRLNVFPLALTPLRERRDDILPLASHLLQQRARPGHRIPALSADVAHLLLT
                     YSWPGNVRELDNLLQRAVVLNEGTVIEAEHILFEKQPMRADGAASTSPVLQQTLQRAE
                     RDVILEALRAAHGSRREVAEKLGISPRTLRYKLARIRNAGIDVPAA"
     gene            1959..2300
                     /gene="fliE"
                     /locus_tag="VIV63_11635"
     CDS             1959..2300
                     /gene="fliE"
                     /locus_tag="VIV63_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY6125292.1"
                     /translation="MSNMEIDRVLAQIRSVSQGGGVGGIGGTKGPAPGGPGAVDNGFA
                     KLLKQGLDSVNQVQNKATTMSAQFERGVPGVDLPQVMLEMQKANVSFRALTEVRNKFV
                     EAYREIMNMPL"
     gene            2321..3979
                     /gene="fliF"
                     /locus_tag="VIV63_11640"
     CDS             2321..3979
                     /gene="fliF"
                     /locus_tag="VIV63_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY6125293.1"
                     /translation="MAAELVNATGSERVISGMKPLLLLVGVAAAIAAGVGVMLWSVGP
                     TYSLLYSNLAAEDTAQITQALEGAGIPYKLENGVGSISVPAEQLAPARLRLASQGLPE
                     GGGGVNAMTRDPGFGVSAFMENARYQHALETELARTIASLQNVQGARVHLAMARQSAF
                     VSERKPGSASVFLQIKAGRRLDDENVQAIANLVASSIPELSASQVTVVDQTGRLLSSP
                     ETDSDTAMRDKMFEFSHRLEESYSNRIQEMLTPLVGPGRVRAQVVAQVDMTTTESTRE
                     QFNPQSQVVRSESTAEEAARNGAGNAGGVPGALTNQPPQPGVALAPGQTPAVAQSGAT
                     PAGATGANASNNTGTTVNGPDSTSRQSTRNYEIDRTMAYTRAPAGRVTRLSVAVLIDN
                     LRTTAADGKVTETPVPPEQLDRLTALVRDAVGFDATRGDTVNVVNSSFLGTPAIDEGE
                     LESIPFWERAWAQTLAKVLAGVLVLLVIVFSVLKPLTKGLLTAARPSVLRQAALAAAT
                     VGGAPAGEAPTLAYEQQVAAARGMVAQDPKRGAQVVKAWVQADG"
     gene            3972..>4794
                     /gene="fliG"
                     /locus_tag="VIV63_11645"
     CDS             3972..>4794
                     /gene="fliG"
                     /locus_tag="VIV63_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEY6125294.1"
                     /translation="MAEIAKSRNGVERAAILLLTLGESEAAEILKHMGAKDVQRLGRS
                     MAELTSVSRDEVRNVLSTFTQELESQTSLGVGSDEFVRKVLVNALGDEKANSLMDRIR
                     LGGQRKGLEALKWMDSRAVAELVRNEHPQIISIVLSYLEPDQGAEVLGFLPENMRSDL
                     IMRVATLDGVQPSALSELDEMMEKQFAAGASNGKSSSLGGLKVAAEMVNLLDAKVGTA
                     VMTEVGKHDERLSQSIQDLMFVFADLMEVDDRGMQELLRQVPADKLLLALKGADET"
BASE COUNT          870 a         1588 c         1561 g          775 t
ORIGIN      
        1 cacgctgttc ctcgacgaga tcggcgacat gagcctgccg atgcaggtga agttgctgcg
       61 cgtcctgcag gagcgcgtgt acgagcgcgt cggcagcaac gtgtcgagcc actgcgatgt
      121 acgcatcatc gccgccacgc accgcaacct cgaagaatcc atcgtgcgcg gcaacttccg
      181 cggcgacctg ttctaccgcc tcaacgtatt ccccatcgag atgccgacgc tcgcgacgcg
      241 catcgacgac ctgcccgtac tcatcgaaga tttcgtgtcg gtcgccgcct cgcgtggccg
      301 tccgcgcctg cagctcctgc ccgacaccat cgatgcgctg tcggcgtatt cctggcccgg
      361 caacatccgc gaactttcga atctcatcga gcgcctcgcg atcctgtgtc ccgagcgccc
      421 ggtgggtgtc ggcgatctgc cggcgcgtta tcgccccgcg gactggaccg ctccggaact
      481 cgtgctgacg ccgacgctgg agattccgca attgctggct tcgatgtccg gcgatgccga
      541 gatcgaagaa gacgaagtgg tgccgacgtc gatgtgggtc ggcgagcagg acgccatcac
      601 gcagttgccg gagcagggcg tcgacctgcg cgcgcatctc atggtcatcg aacgcagcct
      661 catcaatcag gcactgcagc gttccggcgg cacggtcgcg cacgcggcac gactcttgtc
      721 gcttcgccgt actactttgg tggaaaaact gcgcaagctc ggcatggccc agacggccga
      781 gcccgcctga gtttcgtcgc cgtgcgcgcc atcagcgccg gcacctccct ctctccgcga
      841 cggattcatg acactcgtgt gaacggtgtc acgctgcctg agcactcttt gccaggcaca
      901 gctcttgcaa ctgcaatcgt tggccgctga ggccaattga acttgaggtt ccgatgagca
      961 ccgaaatgaa actgagcgac gcgctggaac cagttgcagt cgattcatcc tcgctactga
     1021 ccatggcatt ggcgcggcgt gtggcggtca ccgactgttc ggtgctgatc gtcggcgagt
     1081 ccggcaccgg caaggaagtg ctggcgaggc tcatccaccg tgccagtccg cgcgcgaaga
     1141 acaacttcgt tgccgtcaac tgcgccgcca tccccgagaa catgctcgaa gccatgttgt
     1201 tcggctacga aaagggatcc ttcaccggcg ccgtgaacgc ccacgcgggc aagttcgaac
     1261 aggcgcaggg cggaacgatc ctgctcgacg aaatcaccga gatgccgctg gcgctgcagg
     1321 caaagctgtt gcgcgtgctg caggaacgcg aagtcgaacg actgggcggc aatacgccgg
     1381 tggcgctcga cgtgcgcgtc atcgcgacga cgaaccggct gctgcgagac gaagtcgccg
     1441 cgggccgctt ccgcgaagac ctctattacc gtctcaacgt attccccctg gcgctgacgc
     1501 cgctgcgtga gcgccgcgac gacatcctgc cgctcgcatc gcacctgttg cagcagcgcg
     1561 cgcgcccggg acatcgcatc ccggcactgt cggccgacgt tgcgcatctc ctgctcacct
     1621 atagctggcc cggcaacgtg cgcgagctcg acaaccttct gcagcgcgcc gtcgtgctca
     1681 acgaaggcac ggtcatcgaa gccgaacaca tccttttcga gaagcagccg atgcgcgccg
     1741 atggcgcggc gagcacgtcg ccggtattgc agcagacgct gcagcgcgcc gagcgcgacg
     1801 tgattctcga ggcattacgc gcggcgcatg gcagccggcg cgaagtggcg gagaagttag
     1861 gcatcagccc gcgcacgctg cgctacaagc tggcgcgcat ccgtaacgcc ggcatcgacg
     1921 tacccgcggc ctgactccgg tagatcggga gacttcagat gagcaacatg gaaatcgacc
     1981 gtgtgctggc gcagatccgc agcgtttcgc agggcggtgg cgtcggcggc atcggcggga
     2041 cgaaaggacc ggcacccggt ggtcccggcg ccgtcgacaa cggcttcgcg aagctgctga
     2101 agcagggtct cgactcggtc aaccaggtgc agaacaaggc cacgacgatg tcggcccagt
     2161 tcgagcgcgg cgtgcccggc gtcgatctgc cgcaggtcat gctcgagatg cagaaggcaa
     2221 acgtttcgtt tcgcgctctc acggaggtcc gcaacaagtt cgttgaggcc taccgcgaga
     2281 tcatgaacat gccgctttga gcggctagag gaatcagtcg atggcggcag aattggtcaa
     2341 tgcaaccgga tccgaacgcg tcatcagcgg catgaagccg ctgttgctgc tggtcggtgt
     2401 cgcggccgcg atcgcggccg gcgtcggcgt catgctctgg tcggtagggc ccacctacag
     2461 tcttctctat tccaatctcg cggccgaaga cacggcgcag atcacgcagg cgctcgaagg
     2521 cgccggcatt ccttacaagc tcgagaacgg cgtcggctcg atcagcgtgc cggccgagca
     2581 gctcgcgccg gcgcggttgc gcctggcgtc gcagggcctg cccgaaggcg gcggtggcgt
     2641 gaatgccatg acgcgcgatc cgggtttcgg cgtcagcgcg ttcatggaga acgcgcgtta
     2701 tcagcacgcg ctcgagaccg aactcgcacg caccatcgcc agcctgcaga acgtacaggg
     2761 cgcgcgtgtg catctcgcca tggcgcggca gtccgcgttc gtgagcgagc gcaagccggg
     2821 cagcgcctcg gtattcctgc agatcaaggc cggccgccgt ctcgatgacg agaacgtcca
     2881 ggccatcgcg aatctcgtgg cgtcgagcat tcccgagcta tcggcttccc aggtgacggt
     2941 ggtggatcag accggccgcc tgctgtcttc gcccgagacc gacagcgaca ccgcgatgcg
     3001 cgacaagatg ttcgagttct cgcatcgcct cgaagaaagt tattcgaacc gcatccagga
     3061 gatgctgacc ccgctcgtcg gcccgggccg cgtgcgcgcg caggtcgtgg cgcaggtcga
     3121 catgaccacc acggaatcca cgcgcgagca gttcaatccg cagagccagg tggtgcgcag
     3181 cgaatccacg gccgaagagg ccgcgcgcaa cggcgccggc aatgccggtg gcgtcccggg
     3241 cgcgctgacc aatcaaccgc cgcagcctgg cgtggccctc gcgcctggcc agacgccggc
     3301 cgtggcgcag tcgggtgcta cgcctgccgg cgccacgggc gcgaacgcgt caaacaacac
     3361 gggcacgacg gtcaacggac cggattcgac ctcgcgccag tcgacgcgca actacgaaat
     3421 cgaccgcacc atggcttaca cgcgtgcgcc ggccggacgc gtcacacgtc tgtcggtcgc
     3481 ggtactcatc gacaacctgc gcaccacggc cgccgacggc aaggtcactg aaacgccggt
     3541 gccgccggaa cagctcgacc gtctcacggc gctggttcgc gacgccgtcg gcttcgacgc
     3601 tactcgcggc gacaccgtca acgtcgtcaa ctcctcattc ctcggaacgc cggccatcga
     3661 cgaaggcgag ctcgagtcga taccgttctg ggaacgcgcc tgggcgcaga cgctggccaa
     3721 ggtgcttgcc ggtgttctcg tattgctcgt gatcgtgttc agtgtgctga agcctttgac
     3781 gaagggactg ctgaccgccg cgcgcccctc ggtgctgcgt caggcggcgc tcgccgccgc
     3841 gaccgtcggc ggtgccccgg caggcgaggc tccgacgctc gcctacgaac aacaggtggc
     3901 cgcggcccgc ggcatggtcg cgcaggatcc gaagcgcggc gcgcaggtcg tgaaagcgtg
     3961 ggtgcaggcc gatggctgag atcgccaaga gccgtaacgg cgtcgaacgc gccgccatcc
     4021 tgctgctgac gctcggtgag agcgaagccg cggaaatcct caagcacatg ggcgcgaagg
     4081 acgtgcagcg tctcggccgc agcatggccg agctcaccag cgtgtcgcgc gatgaagtgc
     4141 gcaatgtgct cagcaccttc acgcaggaac tcgaaagcca gacttcgctc ggcgtcggca
     4201 gcgacgaatt cgtgcgcaag gtgctggtca atgcgctggg cgacgaaaag gccaattcgc
     4261 tcatggatcg catccggctc ggcggccagc gcaagggcct cgaagcgctc aaatggatgg
     4321 actcgcgcgc ggtggcggaa ctcgtgcgca atgaacaccc gcagatcatc tccatcgtgt
     4381 tgtcgtatct cgaacccgac cagggcgccg aggtgctggg cttcctgccg gagaacatgc
     4441 gctcggacct gatcatgcgc gtagccacgc tcgatggcgt gcagccgtcg gcgctctccg
     4501 agctcgacga gatgatggag aagcaattcg cggcgggcgc cagcaatggc aagagctcgt
     4561 cgctcggcgg cctcaaggtc gcggccgaga tggtcaacct gctggacgcc aaggtcggca
     4621 cggccgtcat gaccgaggtc ggcaaacacg acgaacggct gtcgcagagc atccaggacc
     4681 tgatgttcgt gttcgccgat ctcatggaag tcgacgaccg cggcatgcag gaactgctgc
     4741 gccaggtgcc ggcggacaaa ttgctgctgg cgctcaaggg agccgacgaa acct
//
LOCUS       DATDEU010000489        11507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_675917, whole genome shotgun sequence.
ACCESSION   DATDEU010000489 DATDEU010000000
VERSION     DATDEU010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675917"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VIV63_11650"
     CDS             <1..1096
                     /locus_tag="VIV63_11650"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEY6125295.1"
                     /translation="EAERVRTQVAGLESKLADFKRANAGQLPELTEVNMSIMDRTESQ
                     LEQVTQQMSSLRQERVFLAAQLEQTRAAGPEAGSVRQLEDQYSRMRSSYDESHPDMVQ
                     LRRQIDSLKFGTSAGSGTSLRSQLNAKRATLAEARQRYGAEHPDIKRLQRDIATLETR
                     IKNGERGDVEMSDGTPVGMQLRTQINAIDSQIGSLMARSAELRAKMANLEQNVTAAPQ
                     VEREYANATRDLTIAREKYEQLLNRQMDAEVSEAAIVGGRADEFRLIQAPMLPGAPGK
                     PQRLAILLIGLVASVVLGLTITVGAEALDPKVRGARDIRELLSVSPLVAIPAIRNSRS
                     RRRNAWRFVTATASGVVGVWIVFTAIQNYL"
     gene            1126..1944
                     /locus_tag="VIV63_11655"
     CDS             1126..1944
                     /locus_tag="VIV63_11655"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEY6125296.1"
                     /translation="MEDAIRRNFADRPGAERPNNLESANLRQRVRRVTAAPAELQQAR
                     RFRQASVDARAMERQCILPAVSDVAALRAFKILRTRVLRRMEMHKWNSIAVSGMSVGE
                     GKTMTAINLALALSQDVNTWVFLVDLDLQRPQVANYLGMSFEKGLSDYLLGKAELEDV
                     VYNLQSERLGVIPNARAFQHSSEFLNSPRMGEFTAALEREMPRRIVIFDMPPLLASDD
                     VLAFAPQFDSLLLVVSEGKSGRAMLRGAKEILSEMNLLGVALNRSKERNESAYY"
     gene            1931..3370
                     /locus_tag="VIV63_11660"
     CDS             1931..3370
                     /locus_tag="VIV63_11660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY6125297.1"
                     /translation="MLTTKDSGAANEELGSGTPVLERIGADENGARPLANNAAVLKRI
                     SIFGLGYVGAVSLACLARDGHQVTGVDIDPVKLDLIRNRKSPILEEGIQELMRDVVNS
                     GRVTVTNDAMQAMRDTELSFVCVGTPSAANGSQDLTAILRLAEQIGSALALKDEYHTI
                     VIRSTVQPGTVEEKIEPILERASGKKSGVDFGLCFQPEFLREGSSIRDYDHPPYTIVG
                     GNGEAAVNAVREVFQHLDSRFLVTNVRVAETLKMACNAFHALKITFANEIGRVSQSMG
                     IDGHEVMRLVCADKRLNISPAYLKPGFAFGGSCLPKDLRALTNIAKQNDVVLPMLSSL
                     LASNRVHIDHAVEKILKLGRPKVGMLGLSFKTGTDDLRESPLVLVAKRLLGEGCELRI
                     FDPEVQLSRLLGANRSYIDANIPHLGSLLCGTIEEMMEPSDVILVGLQQSVLNDALQA
                     HVRPEQYVLDLVNLPNRDLLRCRYEGVCW"
     gene            3679..4311
                     /locus_tag="VIV63_11665"
     CDS             3679..4311
                     /locus_tag="VIV63_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY6125298.1"
                     /translation="MCNGCGGGGGSSDTAAPAAASPPPVTAPAVTNRAPTISGQVIDS
                     VRVGETYVWQPVAADPDGDAMHFSATNLPPWASLDPTSGVISGTPGETDVGVYEEITI
                     TVADAGQQAATSPFSITVIGDVGTGVASLQWETPPSKVDGSPLDDLAGFRILYGRNSD
                     DLDHSVIIDSPSTTSYDFTSLTTGIWYFTVVAVNAGGLEGPPTTVATKSI"
     gene            4394..5785
                     /locus_tag="VIV63_11670"
     CDS             4394..5785
                     /locus_tag="VIV63_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125299.1"
                     /translation="MKPLALLVLGLVVIGATTAQAIPVIPGAAGYGIDTPAGRGGTVY
                     KVTNLSASGTGSLKACVDGTTPRVCVFEVSGTIRLTADLTIRNSNLTIAGQTAPSPGI
                     MIRGAGIKIQASDVLIQHLRVRAGDDVNGPDPDNRDSLKIEGTTAKPVRNVVIDHCSF
                     SWALDETASVWGPHDNITFSNNIFAEPLNESMHPDYDGGGVMKHGFGVLIGTAPNSSV
                     TMVGNLMAHQVERNPLSRASEFVFVNNLVYGATTRELDLQSEAGRVTRNSVVGNVFVQ
                     GPVHSNTAKPIFVRTNGSLTLGLGSRVYVHDNTSPETGSSYSSLVLLTGGDILSGLMS
                     QTTAPVWNTGLVARPVASSGVYNNVLKFAGARPTDRDTVDKRVVATVKNRNGQVINCV
                     SSNGTTRCNKNAGGWPSLAQNVRRLTLPANPNSTASNGYTNLENWLHSMDLNVAGVVA
                     ATSPTSPPALSVR"
     gene            5909..7342
                     /locus_tag="VIV63_11675"
     CDS             5909..7342
                     /locus_tag="VIV63_11675"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY6125300.1"
                     /translation="MNTEYQDQISQPIYFKRGMSFAAPVMLWLLEALPAVLAGVMFFP
                     LTQIYDVEFDRPFVVLSILAATLTLAFLPTRNPTRQIVSSRLELATHLLFRWAVLVAA
                     LLALGYMTKFSESFSRRVVVTWVLVTPVLLVMLSLILQSVARALMRDAAQARRAIIVG
                     CTQASVELSKRLALHVELGISVAGFFDDRGSDRLACSKHAQLLGRFDDVASFVNGRNI
                     DVIFVALAPGQVGRVRELVHELGDTTASIYFLPDVSGFDCIQQRASEILGMPVVAMCE
                     TPFHGYRGLIKRLMDITIASMAVVVLAPVLLAVAIAVKRSSPGPVLFRQRRYGLDGRE
                     INVYKFRTMTVCENGALVTQASRNDIRVTPVGKHLRRWSLDELPQLLNVLQGTMSIVG
                     PRPHAVAHNEEYRKLIKRYMVRHKVLPGITGLAQVRGCRGETARVEHMEARVLFDLEY
                     MRRWSPMLDLEIILATVFAVIRTDNAY"
     gene            7402..8703
                     /locus_tag="VIV63_11680"
     CDS             7402..8703
                     /locus_tag="VIV63_11680"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY6125301.1"
                     /translation="MPTILGFAALFLLATDALNLDMSLLPGVSAKNLIIYLIAVVLAL
                     RMVVSRSSIMIVGQLQGAFIIQIGYAIITWLIAALLIQYPYYDLFASGIKLKSALIDH
                     YIFFLVFLFGTQTAEDAIKVGKGLLFGALLANIITILDAAGIVSLGFRIREDGRTAGA
                     IGESNQYAAFIILFLPATVAAAVAAHGLRRLFWFGAALVGCMTLVMTASRGGFVGLAM
                     MCAVGAYLYRHLISYSRISGWVFGSLIMLVLVVSFSQYGNLLAERMIGTGSIDASTAS
                     SGRSEIWADALAVMFAHPITFITGFGWDAYSVMPFQFSPHNHYLALWFNLGLVGLLCG
                     SYLLFSSIGRARRASLHAAPPLRGQLIAFVLGTVGVCTAVFFVDLHKPWFYFWMYAGV
                     AMRMVLCVEKNPVVETAAAPRTRRPSVRRDPYGWANAGTRS"
     gene            8700..9863
                     /locus_tag="VIV63_11685"
     CDS             8700..9863
                     /locus_tag="VIV63_11685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6125302.1"
                     /translation="MSARRICIVGLDSYGMLSGEGNPKYIGGESIQHVLLARAWRDLG
                     HDVSRIVHDEGQGARREHDGITAIAAHTRHGGMPGLRFFHPRATRLVSAMIAADADIY
                     YQSPAGAYTGITAWFCRATGRRFIFRVASDSDCEKEHGRIQFWRDRKLYNYGLRHADL
                     VAAQTEFQAQTLRENHEVESAVINMMVEPPRGSAPRDKDIDVLWVSNLRALKRPELAL
                     ELARQLPQVKFSLAGGPMPGGETYFEDVRAAAARLPNVTMHGAVRYADSGALFDRAKI
                     FLNTSSIEGFPNTFLQAWIRGVPVVSFFDPDSLVQRLQLGHIANSVDEMREAIRSLLE
                     DDAERQLTGRRAREFATREYTTGVAARYLELLNQNSPQKRVSAAHWAKGANLP"
     gene            9860..11050
                     /locus_tag="VIV63_11690"
     CDS             9860..11050
                     /locus_tag="VIV63_11690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6125303.1"
                     /translation="MNNPTCLAPAMKETLSLVTPRETHDVVTPACDALFVLSSLAVGG
                     SERKIARMANRLKEDGVAVNLACLNAPYTLESTLRRDVPLTKLERRGKFSFATVLKLR
                     QMIVRDRPATVVAVNLYQALYVACATLFSPKRPRIVALVNTSTFRGRHFRKWLYQTVL
                     SRFDLVVHGSQAQTRFWEGPDRSTVIYNGVDSTHFDPIESFEAAKRLRASLGVKPGAL
                     LIGSVGRLAPEKNHEVLLTTLRRLRVARVDAHLVIAGSGPLREQLLRRAAELEIADRV
                     NLIGEFEDVRPVLAAMDLFVLPSVAVESFSNAALEAMSMGKPVILSDIGGAREMIDDG
                     VEGYVVSATELSARLPAIIAALYADTRKRQGMGAAARARAMSRFSVAGMAAAYRGLLH
                     GEVS"
     gene            11050..>11507
                     /locus_tag="VIV63_11695"
     CDS             11050..>11507
                     /locus_tag="VIV63_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125304.1"
                     /translation="MSADATTVLLPRAAAPRAPSPREGRVKVLQMGPALDVRGGVSSV
                     EQLICDYLPPYASIRHVATMTEGTKFANAVVFARALRTLSRTLDGIDPTIVHIHFASR
                     GSTLRKFILANMVVRARRPLILHAHGAGFDQFYRGLPAPLRSVVNRTLQQA"
BASE COUNT         2049 a         3776 c         3565 g         2117 t
ORIGIN      
        1 ggaagccgag cgggttcgca cacaggtggc gggcctcgag tccaaactcg ccgacttcaa
       61 gcgagccaac gcgggccagc ttccggaact gacggaagtg aacatgtcga tcatggatcg
      121 caccgagagc cagctcgagc aggtcactca gcagatgagt tcgctgcgcc aggaacgcgt
      181 gttcctcgcc gcgcaactcg agcagacgcg tgccgcgggt cctgaagccg gcagcgttcg
      241 ccagctcgaa gaccagtact cgcgcatgcg ttcgagctac gacgagagcc atccggacat
      301 ggtgcagctg cgccgccaga tcgacagcct caagttcggc accagtgccg gctcgggcac
      361 gtcgctgcgc tcgcagctca atgccaagcg cgcgacgctg gccgaagcgc gtcagcgtta
      421 cggcgccgag caccctgaca tcaagcggct gcagcgcgac atcgcgacac tcgagacgcg
      481 catcaagaat ggtgagcgtg gcgacgtcga gatgtccgat ggcacgcccg tcggcatgca
      541 gctgcgcacg cagatcaatg ccatcgacag ccagattggc agtctcatgg cccgcagcgc
      601 ggagctgcgc gccaagatgg ccaatctcga gcagaacgtc acggccgcgc cgcaggtgga
      661 acgcgaatac gccaacgcca cgcgcgacct cacgatcgcc cgggagaaat acgagcagct
      721 gctcaatcgc cagatggacg cggaagtgag tgaagcggcg atcgtcggcg gccgcgccga
      781 tgaattccgc ctcatccagg ctcccatgtt gccgggcgct cccggcaagc cgcagcgcct
      841 cgcgatcctg ctgatcggcc tcgtcgcgtc ggtcgtgctc ggtctgacca tcacggtcgg
      901 cgccgaagcg ctcgatccga aagtacgcgg tgcgcgtgac atccgtgaac tgctgtcggt
      961 ttcgccgctg gtcgccattc cggcgatccg caattcgcgc tcccgccggc gtaacgcctg
     1021 gcgcttcgtg accgcgaccg ccagtggcgt ggtcggcgtg tggatcgtgt tcaccgccat
     1081 ccagaactac ctgtgaccca acgagagaca caaatgagta cgaccatgga agatgcaatc
     1141 cgccgcaact tcgccgaccg accgggcgct gaacgcccga acaacctcga gtcagcgaac
     1201 ctgcgccagc gtgtgcgccg cgtgacggcc gcgcccgccg agctgcaaca ggcacggcgc
     1261 ttccgccagg cgagcgtcga tgcgcgcgcc atggaacgcc agtgcatcct gcccgcggtg
     1321 tccgatgtcg cggcgttgcg tgcgttcaag atcctgcgca ctcgtgttct gcgacgcatg
     1381 gagatgcaca agtggaactc catcgccgtg tccggcatgt cggtcggcga aggcaagacc
     1441 atgaccgcga tcaacctcgc gctcgcgctc tcgcaggacg tgaacacctg ggtgttcctc
     1501 gtcgacctcg atctgcagcg accgcaggtc gccaactacc tcggcatgag cttcgagaag
     1561 gggctgtccg actatctgct cggcaaggcc gagctcgagg acgtggtcta caacctgcaa
     1621 tcggagcgcc tcggcgtgat tccgaacgcg cgcgcgttcc agcactcctc ggaattcctg
     1681 aactcgccgc gcatgggcga attcacggcc gcgctcgagc gcgagatgcc gcgccgtatc
     1741 gtgatcttcg acatgccgcc gctgctcgca tcggacgacg tgctggcctt tgcgccgcag
     1801 ttcgattcgc tgctgctggt ggtgtccgaa ggcaagagcg gccgcgccat gctgcgcggc
     1861 gcgaaggaaa tcctgagcga gatgaatctg ctgggtgtgg cgttgaatcg ctcgaaggaa
     1921 agaaacgaaa gtgcttacta ctaaagattc gggggcggcg aacgaagaat tggggtcagg
     1981 caccccagtt cttgaaagaa ttggcgcgga cgaaaacgga gccagacccc tggctaacaa
     2041 cgctgcggtt ctaaagcgga taagcatctt cggccttggc tacgtaggcg ctgtgtcgct
     2101 cgcttgcctc gcaagggacg gtcaccaggt gacgggcgtc gacatcgacc cggtcaaact
     2161 cgacctcatc cgcaaccgca agtcgccgat cctcgaggaa ggcatccagg agctcatgcg
     2221 cgacgtggtc aactcgggcc gcgtgaccgt gaccaacgat gccatgcagg cgatgcgcga
     2281 cacggaactc tcgttcgtgt gcgtgggcac tccgtcggcc gccaacggca gccaggacct
     2341 cacggccatc ctgcgcctcg cggagcagat cggctcggcg ctggcgctca aggacgaata
     2401 ccacaccatc gtgatccgtt cgacggtgca gccgggaacg gtcgaagaga agatcgagcc
     2461 catcctcgag cgcgccagcg gcaagaagtc gggtgtcgac ttcggtctgt gctttcagcc
     2521 ggaattcctg cgcgaaggct cgtcgatccg cgactacgat catccgccgt acaccatcgt
     2581 gggcggcaat ggcgaagcgg cggtcaacgc cgtgcgcgaa gtcttccagc atctcgactc
     2641 ccggtttctc gtgaccaacg tccgcgttgc ggaaaccctg aagatggcct gcaacgcatt
     2701 ccacgcgttg aagatcacgt tcgccaacga gattggccgc gtgtcgcagt ccatgggaat
     2761 cgacgggcac gaagtcatgc gtctcgtgtg cgcggacaag cgcctgaaca tttcgccggc
     2821 ctatctgaag ccgggctttg ccttcggcgg ctcgtgcctg ccgaaggatc tgcgcgccct
     2881 caccaacatt gcgaagcaga acgacgtcgt gctgcccatg ttgtcgagcc tgctcgccag
     2941 caaccgcgtg cacatcgatc atgccgtcga gaagatcctc aagctcggcc ggccgaaggt
     3001 cggcatgtta gggctgtcgt tcaagacggg caccgacgac ctgcgcgaat caccgctggt
     3061 gctggtggcg aagcggctgc tgggcgaggg ctgcgaactg cgcatcttcg acccggaagt
     3121 acagctctcg cgcttgttag gcgccaaccg cagctacatc gatgcgaaca ttccgcacct
     3181 cggctcactg ctgtgcggga ccatcgagga gatgatggaa ccctccgacg tcatcctcgt
     3241 cgggctgcaa cagtccgtgc tcaacgatgc gttgcaggcg cacgtgcggc ccgaacaata
     3301 cgtcctcgac ctcgtgaacc tgccgaatcg cgatctcctg cgttgccgtt acgagggcgt
     3361 ctgctggtaa aggccggccg gcgagtccgg ccttaactac atatataaga gaaggtggag
     3421 cggcaccgct ttggcgcggc ttcacacttt tcgacttgtg caaatctttc aatttcactt
     3481 aaaccgttga aagtattccg ttgtcggtgt ccgccgacgt aggaggagtc gggcagaatt
     3541 cggccgttgc gttgaatcaa ttgccgtcgg ggccgtaaaa ctctccacac cccagcaggg
     3601 gatgtgagag ggctccgaca gatgtttcga ctcaatcgcg gatttgtcag cgcattccta
     3661 ccgttgatcc ctttggccat gtgcaacggg tgcggcggtg gcggcggatc ttcagacacg
     3721 gcagcgccgg ccgcggcttc gccgccgccc gttacggcgc ccgccgtcac gaatcgtgcg
     3781 ccgaccatct ccggccaggt catcgacagc gtgcgcgtgg gtgagaccta cgtgtggcaa
     3841 cccgttgcgg ccgatcccga tggcgacgcg atgcacttca gcgccaccaa tctaccgccc
     3901 tgggccagcc tcgatccgac cagcggcgtc atcagcggca cgcccggcga gaccgacgtg
     3961 ggcgtctatg aagagatcac gatcacggtt gcggacgccg ggcagcaggc cgcgacgtca
     4021 cctttctcga tcacggtgat cggcgacgtc ggcacgggtg tcgcctcgct gcaatgggaa
     4081 acgccgcctt cgaaggtgga cggctcgccg ctcgacgatc tcgccggctt tcgcattctg
     4141 tatggccgca acagcgatga cctcgatcac agcgtgatca tcgacagccc gtccacgact
     4201 tcctatgact tcacgtccct gacgaccggt atctggtact tcacggtggt cgccgtgaat
     4261 gccggtgggc tcgagggccc gccgaccacc gttgccacca agtccatctg acaaccgtcg
     4321 taatgaattc cgagaggacc ctgtaatgaa gcttgctagt gtctgcagcc accatgcctc
     4381 cacgcaccgc atcttgaagc ccctggcctt gttggtgctt ggcctggtcg tcatcggcgc
     4441 cacgaccgcc caggcgatcc cggtcattcc aggcgcggcc ggatatggca tcgacacgcc
     4501 cgctggtcgc ggcggcaccg tgtacaaggt gacgaacctc agtgcgagcg gaaccgggtc
     4561 gctgaaggcc tgcgtcgacg gcaccacgcc gcgcgtttgc gtgttcgaag tttccggaac
     4621 gatccgtctg accgccgacc tcacgatccg caacagcaat ctgacgattg ccggccagac
     4681 agcgccgtcg ccgggcatca tgattcgcgg cgcgggcatc aagatccagg cgtcggacgt
     4741 gctcatccag cacctgcgcg tacgcgcggg cgatgacgtg aacggaccgg atccggacaa
     4801 ccgcgattcg ctcaagatcg aaggcaccac ggcgaagccg gtgcgcaatg tcgtcatcga
     4861 ccattgctcg ttcagctggg cgctcgacga gaccgccagc gtctggggtc cgcacgacaa
     4921 catcacgttc agcaacaaca tcttcgcgga gccgctcaac gagtcgatgc atccggacta
     4981 cgatggcggc ggcgtgatga aacacggctt cggtgtcctc atcggcacgg cccccaacag
     5041 cagcgtcacg atggtgggca atctcatggc gcaccaggtc gagcgcaacc cgctatcacg
     5101 cgcctccgaa ttcgttttcg tgaacaacct cgtctatggc gccaccacgc gcgaactcga
     5161 tctgcagagc gaggcggggc gcgtgacgcg gaattcggtg gtcggcaacg tcttcgtgca
     5221 aggacccgtc cacagcaata ccgccaaacc cattttcgta cgcaccaacg gcagcctgac
     5281 tctgggtctg ggtagccgcg tatacgtgca cgacaatact tcgccggaaa ccggcagctc
     5341 ctattcgtcg ctggtgttgc tcacgggcgg ggacatcctg tccggcctca tgtcgcagac
     5401 caccgcaccg gtatggaaca caggtctcgt ggcacgcccg gttgcgagca gtggcgttta
     5461 caacaacgtt ctcaagttcg ccggtgcgcg tcctacagac cgcgacaccg tcgacaagcg
     5521 tgtcgtggcg accgtcaaga accgcaatgg tcaggtcatc aactgcgtgt cgtcgaacgg
     5581 caccacccgc tgcaacaaaa acgcgggtgg atggccctcc ttggcgcaaa acgtgcgccg
     5641 gctgacgttg cccgcgaatc ccaacagcac ggcgtcgaat ggctacacca atctcgagaa
     5701 ctggttgcac tccatggacc tcaatgtcgc cggcgttgta gccgcgacga gtccgacatc
     5761 acccccggcg ctgtcagtgc gttgatgacg taagcaggac aattcgaggg acgtgtaatc
     5821 gtccggcgat acggcatgaa gacaagggct ggcgttcaaa cgaccagccc ttttctattc
     5881 tgtcggcgtg ttgggaaggg acgcgccgtt gaataccgaa tatcaggatc agatcagtca
     5941 gcccatttac ttcaagcgcg gcatgtcatt tgccgcgccc gtcatgctgt ggctgctcga
     6001 agccttgccg gccgtgctcg ccggcgtgat gttctttccc ctcacgcaaa tctacgatgt
     6061 cgaattcgac cgtcctttcg tggtgctgtc gatactcgcc gccacgctga cgctggcctt
     6121 cctgccgacg cgtaatccca cgcggcagat cgtctccagc cgcctcgagc tcgccaccca
     6181 tctcctgttc cgctgggcgg tgctggtcgc cgcgttgctg gcgctcggct acatgaccaa
     6241 gttctcggag agcttctcgc gccgcgtcgt cgtcacctgg gtgctggtga cgccggtact
     6301 gctggtgatg ctctcgctga tcctgcagag cgtggcgcgt gcactcatgc gcgatgccgc
     6361 gcaggcgcgc cgcgcgatca tcgttggctg cacgcaggcg agcgtggagc tgtccaagcg
     6421 cctcgcgctt cacgtcgagc tcggcatttc ggtggccggg ttcttcgacg atcgcggcag
     6481 cgaccgcctg gcctgctcga aacacgctca gttgctgggc cgcttcgatg acgtcgcctc
     6541 gttcgtcaac ggccgcaata tcgacgtcat ctttgtcgcg ctggcgccgg ggcaggtggg
     6601 ccgcgtgcgc gaactcgtgc acgagctcgg cgacaccacg gcgtccatct acttcctgcc
     6661 cgacgtctcg ggattcgact gcatccagca gcgcgcgagc gagatcctcg gcatgcccgt
     6721 ggtcgcgatg tgcgaaacgc cgttccacgg ttaccgcggc ctcatcaagc ggctcatgga
     6781 catcacgatc gcgagcatgg cggtggtcgt gctcgcgccg gttctgctcg ccgtcgccat
     6841 cgcggtcaaa cgcagctcgc ccggtcctgt cttattccgg cagcgccgct acggcctcga
     6901 cggccgcgaa atcaacgtct acaaattccg cacgatgacg gtgtgcgaaa acggcgcgct
     6961 ggtgactcag gcgtcgcgaa acgacatccg cgtgacgcct gtcggaaaac atctgcgacg
     7021 ctggtcgctc gacgaactac cgcagctgct caacgtgctg cagggcacga tgagcatcgt
     7081 aggaccacgc ccacatgctg tcgcgcacaa cgaggaatac cgcaagctga tcaaacggta
     7141 catggtgcgc cacaaggttc ttccgggaat tacgggtttg gcgcaggtgc gtggatgccg
     7201 tggcgaaacc gcccgggtgg aacacatgga agcgcgcgtc ctgttcgatc tggaatacat
     7261 gcgtcgatgg tcacccatgc tggacctgga aatcatcctg gccaccgtgt tcgctgtgat
     7321 caggacggac aacgcgtact gacatcgtca tcaaggaaca acatcatttc cgtgttcaca
     7381 gttttcggac gagtctgacg catgccgaca attctcggat tcgccgcgtt gttcctgctg
     7441 gcaaccgatg ctctcaacct ggatatgagt cttctgccgg gtgtatcggc caagaacctc
     7501 atcatttatc tgatcgcggt cgtcctcgca ctgcgcatgg tcgtcagccg ctcgtccatc
     7561 atgatcgtcg gccagctcca gggcgccttc atcatccaga tcggctacgc gatcatcacg
     7621 tggctgatcg ccgcgctgct catccagtat ccgtactacg acctgttcgc gagcggcatc
     7681 aagctcaaga gcgcgctcat cgatcattac atcttcttcc tcgtgttcct gttcggcacg
     7741 cagacggctg aagacgcgat caaggtgggc aagggcctgc tgttcggcgc gttgctcgcc
     7801 aacatcatca cgatcctgga cgccgccggc atcgtcagcc tcggcttccg catccgcgaa
     7861 gacggccgca ccgcgggcgc catcggcgag tccaaccaat acgccgcctt catcatcctg
     7921 ttcctgccgg cgaccgttgc cgcagcggtg gccgcgcacg gactacgccg gttgttctgg
     7981 ttcggcgccg cgctggtcgg ctgcatgacg ctggtcatga cggcctcgcg cggcggcttc
     8041 gtcggtctgg ccatgatgtg cgcggtgggc gcctatctgt atcgccatct catctcctac
     8101 agccggatct cgggctgggt gttcggctcg ctgatcatgc tggtgctggt cgtgtcgttc
     8161 tcgcagtacg gcaacctgct cgccgagcgc atgatcggca cgggctccat cgacgccagc
     8221 accgcatcgt cggggcgcag tgaaatctgg gccgatgccc tggccgtgat gttcgcgcat
     8281 cccatcacgt tcatcaccgg cttcggctgg gatgcgtatt cggtcatgcc gttccagttc
     8341 tcgccgcaca accactacct ggcgctgtgg ttcaaccttg gcctcgtggg cctgttgtgc
     8401 ggatcctatc tactgttctc gtcgatcggc cgcgctcgcc gggccagcct gcacgccgcg
     8461 ccgccgctcc gcggccagct catcgcgttc gtgctcggca cggtcggtgt ctgcacggcc
     8521 gtcttcttcg tggacctgca caaaccctgg ttctatttct ggatgtacgc cggcgtcgcc
     8581 atgcgcatgg tgctgtgtgt ggagaagaac ccggtcgtcg aaaccgccgc ggcgccgcgc
     8641 acgcgacggc cgtcggtgcg ccgcgacccg tatggctggg cgaatgcggg gacgcgttca
     8701 tgagtgcccg ccgcatctgc atcgtgggtc tcgattcgta cggcatgttg tcgggcgaag
     8761 ggaatccgaa atacatcggc ggcgagtcca tccagcacgt gctgctggcg cgcgcatggc
     8821 gcgatctcgg acacgacgtg tcgaggatcg tccacgacga agggcagggc gcgcgccggg
     8881 aacacgacgg catcaccgcc atcgccgcgc atacacgcca tggcggcatg ccgggcttgc
     8941 gtttctttca tccgcgcgcc actcgcctgg tgtcggcgat gatcgccgcg gacgcggaca
     9001 tctattacca atctcccgcg ggtgcgtaca cgggcatcac cgcgtggttc tgccgcgcca
     9061 cggggcgtcg attcatcttt cgcgtcgcgt cggacagcga ctgcgaaaag gaacatggcc
     9121 gcatccagtt ctggcgcgat cgcaagctgt acaactacgg actgcggcac gccgacctcg
     9181 tggccgcgca aacggaattc caggcgcaga cgctgcgcga gaatcacgaa gtcgaaagcg
     9241 ccgtcatcaa catgatggtc gagccgccgc gcggatccgc tccgcgcgac aaggacatcg
     9301 acgtgttatg ggtcagcaat ctgcgcgcac tcaagcggcc ggagctcgca ctcgagctcg
     9361 cgcgtcagtt gccgcaggtg aagttctcgc tcgccggcgg cccgatgccc ggtggtgaga
     9421 catatttcga agacgtgcgt gctgctgcag cgcggttgcc caacgtcacg atgcacggag
     9481 cagtgcgtta cgcagatagc ggagcactgt tcgatcgcgc gaagatcttt ctcaacacgt
     9541 cgagcatcga gggattccct aatactttcc tgcaagcgtg gattcgcggc gttccggtgg
     9601 tctcgttctt cgatccggat tcgctggtgc aacgcctgca gctcggccac atcgcaaact
     9661 cggtcgatga aatgcgcgaa gccatccgca gcctgctcga agacgatgcc gagcgccagc
     9721 tgaccgggcg ccgtgcgcgc gagttcgcca cgcgcgagta caccacgggg gttgccgccc
     9781 gttacctcga actactgaat cagaattcgc ctcagaagcg agtgagcgct gctcattggg
     9841 caaagggagc caatctgcca tgaacaatcc aacctgcctt gcacccgcca tgaaagaaac
     9901 gctgtcactc gtgactccgc gtgagacaca cgatgtggtc acgccggcct gcgatgcgct
     9961 cttcgtgctc agcagccttg cggtgggagg gtcggagcgc aagatcgcgc gcatggccaa
    10021 ccgcctcaag gaagacggag tggccgtgaa cctggcctgt ctcaacgcgc cgtacacact
    10081 cgaatcgact ctgcgtcgcg acgttccgct gaccaagctc gagcgccgcg gcaagttctc
    10141 ttttgccacc gtgctgaagc tgcggcagat gatcgtgcgc gaccgccccg cgaccgtggt
    10201 tgcggtgaac ctgtaccagg cgctgtatgt cgcctgcgcg acgctgttca gccccaagcg
    10261 gccgcgcatc gtggcgctgg tgaacacgtc gacgttccgg ggacgccact tccgcaagtg
    10321 gctctaccag accgtgctct cgcgtttcga tctcgtggtg cacggctcgc aggcgcagac
    10381 gcgtttctgg gaagggcccg accgatccac cgtcatctac aacggcgtgg attccacgca
    10441 cttcgatccc atcgagtcct tcgaagccgc gaaacgattg cgcgcctcgc tcggcgtcaa
    10501 gcctggcgcg ttgctcatcg gcagtgtcgg tcggctcgcg cccgagaaga atcacgaagt
    10561 gctgctgacg acgctgcgcc gcctgcgcgt ggcacgcgtc gatgcgcacc tggtgatcgc
    10621 cggcagcggt ccgctgcgcg aacaactgtt gcggcgcgcc gccgaactgg aaatcgccga
    10681 tcgcgtgaat ctgatcggcg aattcgaaga cgtgcgtccg gtactggccg ccatggatct
    10741 gttcgtgttg ccgtcggtcg ccgtggaatc cttttcgaac gccgcgctcg aggccatgtc
    10801 catgggcaag cccgtgatcc tctcggacat cggcggcgcc cgcgagatga tcgacgacgg
    10861 cgtcgaaggc tacgtggtct cggccaccga gctcagcgcg cggctgccgg ccatcatcgc
    10921 cgcgctgtat gccgacacgc gcaagcgaca ggggatggga gcggcggcgc gtgcgcgcgc
    10981 catgagccgc ttctcggtcg ccggcatggc ggcggcgtat cgcggtctgc ttcatggtga
    11041 ggtttcctga tgagcgccga tgccactacc gtcctcctgc cgagagccgc cgcgccgcgt
    11101 gcgccatcgc cacgggaagg ccgcgtgaag gtcctgcaga tggggcccgc gctcgacgtg
    11161 cgcggcggcg tgagctccgt cgaacagctc atctgcgact atctgccgcc gtacgcttcc
    11221 attcgtcacg tcgccacgat gaccgagggc acgaaattcg cgaacgccgt ggtgtttgcg
    11281 cgcgcgctgc gcacgctgtc gcgcacgctc gacggaatcg atcccaccat cgtgcacatc
    11341 catttcgcgt cgcgtggcag cacgctgcgc aagttcatcc tcgcgaacat ggtggtgcgt
    11401 gcgcgtcggc cgctcatcct gcacgcgcat ggcgcgggct tcgatcagtt ttatcgcggc
    11461 ctgccggctc cgctgcgcag cgtcgtgaac cgcacgctgc agcaggc
//
LOCUS       DATDEU010000490         2878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1842490, whole genome shotgun sequence.
ACCESSION   DATDEU010000490 DATDEU010000000
VERSION     DATDEU010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842490"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VIV63_11700"
     CDS             <1..713
                     /locus_tag="VIV63_11700"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6125305.1"
                     /translation="GLEAAAALRARKVEVNVVSVDPVPLARVLGPAIGNFIRGVHEEH
                     GVRFQFGSAPRAITSRGVELENGTTLAADFVVVGVGVAPRTSLAEAAGITVDRGVVVD
                     DQFRTSASGVYAAGDIASFPDFRSGARMRIEHWQVAERHGLAVARAMLGIGEPFRDVP
                     FFWSAHYDVTLNYVGHAEKWDRIIERGSLADRKYVAAFEQQGKILAVATLGEDLLSLE
                     VEAVMSAGDEAKVKALVG"
     gene            complement(785..1300)
                     /locus_tag="VIV63_11705"
     CDS             complement(785..1300)
                     /locus_tag="VIV63_11705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY6125306.1"
                     /translation="MPGPRNKRVRILQLGARDEADFISAVRASRKLHQNWVHPPGTRT
                     KFREKVSLMQGPVNLAYCVRRVDNDALVGCIEITQIVRGAFRSAYLGYYAFAGQERQG
                     LMHAGLQHVVRLAFTELGLHRLEANIQPENAPSIALVKACGFRKEGYSPRYLKIRGRW
                     RDHERWAIVAS"
     gene            1533..2012
                     /locus_tag="VIV63_11710"
     CDS             1533..2012
                     /locus_tag="VIV63_11710"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6125307.1"
                     /translation="MALWKDQFGREQSGPEPVAAKAPEKVSVLSAVSDQAPRPAPAPN
                     RSDATMKESIIAAGLTIEGKIEGAGHVRIAGDFKGDVNVQGNLTIEQGAHLTGGVKAD
                     TVVVGGQLDGNIDATSRVELLQTGVFNGELKAGSLTVAAGSRMRGKAEFGWDEKSKH"
     gene            2020..2370
                     /locus_tag="VIV63_11715"
     CDS             2020..2370
                     /locus_tag="VIV63_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125308.1"
                     /translation="MNGRVVAPGKTRICPHCKTSILESASVCPGCHHHLRFDPKAIAE
                     SRAQATFSALRVEGAFRNPEGAESWEYSVVVSIRNDRGEEVARKVVGVGALDARESRT
                     VTLSVDVFAPRSPK"
     gene            complement(2451..>2878)
                     /locus_tag="VIV63_11720"
     CDS             complement(2451..>2878)
                     /locus_tag="VIV63_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125309.1"
                     /translation="FHRPAVFGPAVGFFAFLANEARTSTDSGSADGLLWLPFILLVVT
                     LIAAIPYILGAFLLLASCRVLPASWVGFMAIRLILGGVIGALIAVPFTHMLNWIPSST
                     ADPRFNFESMLVGCVAGGVYCAAFLRIASPRTAGLSPEA"
BASE COUNT          558 a          866 c          940 g          514 t
ORIGIN      
        1 tcggcctgga ggcggcggcg gctctgcgtg cgcggaaggt cgaagtgaac gtcgtgagtg
       61 tcgaccccgt gccgctggcg cgcgtgcttg gtcccgcgat cggcaatttc attcgcggag
      121 ttcacgagga acatggcgtg cggtttcaat tcggtagtgc acccagagca atcaccagtc
      181 ggggcgtcga gctcgagaat ggaacgacgc tggccgcgga cttcgtggtc gttggagttg
      241 gagttgcacc gcgcacttcg ctggccgaag cggcgggcat cacggtcgat cgaggagttg
      301 tcgtcgacga tcaattccgc acgagcgcca gcggtgtcta tgcagcgggc gatatcgcat
      361 catttcccga cttccgcagc ggagcgcgca tgcgcatcga acactggcag gtcgcggagc
      421 ggcacggtct cgcggtcgcg cgcgccatgc tcggcatcgg cgagcctttc cgcgacgtgc
      481 cgttcttctg gagcgcacat tacgacgtca cgctcaacta cgtcggccac gcggagaagt
      541 gggatcgcat catcgagcgc ggcagcctcg cagatcgcaa gtacgtcgcc gcgttcgagc
      601 agcagggaaa gatccttgcc gtcgccacgt taggtgagga tctgttgagt ctcgaggtcg
      661 aggcggtcat gagcgccggc gacgaagcga aggtgaaggc gctcgtcggg tagctagcta
      721 gtacacgtac ctgcggcgaa gtgactggat gaacccttgc cattgaggtg atacctggcg
      781 cctgtcagga agccacgatc gcccagcgct catggtcgcg ccaccggccg cggatcttca
      841 ggtagcgagg cgagtagcct tccttcctga agccgcaggc cttgaccaac gcaatggacg
      901 gcgcgttctc cggttgtata ttcgcctcga gcctgtgcag cccaagctcc gtgaaagcga
      961 gccgcacgac gtgttgaagt cccgcgtgca tcaagccctg tcgctcctgg cccgcgaacg
     1021 cgtaatagcc tagataggcg cttcgaaacg cgccgcgaac gatttgcgtg atctcgatac
     1081 agcccaccag tgcatcgtta tcaacgcgac ggacgcagta ggccaggttc accggaccct
     1141 gcatcaggct gaccttctcg cggaattttg tgcgagtgcc gggcggatgc acccaattct
     1201 ggtgaagctt gcgactcgcg cgcacggcgg agatgaagtc ggcttcgtct ctcgcaccga
     1261 gttgaaggat tcgaacgcgt ttgttcctgg ggcctggcac atggtctcct ggagtggcgg
     1321 ccagaccacc tagttaggcg tcgtctggcg catccctgtc acgctcgaat tcccgccgat
     1381 cggcctgccg ttcctaaggg caacgctggc cagccgcttg tcggcgtatc ccgacatttt
     1441 gctgcgaagt ttgacactcc gcagcgcgct gtgtacttac gttcgacgat ctcacgaggg
     1501 tcgtcaccta tttcaggtac tggaggtttg tcatggcgtt gtggaaagat caatttggcc
     1561 gggagcagag tggaccggaa cccgtcgccg caaaggcgcc cgaaaaggta tccgtactgt
     1621 ccgccgtcag cgaccaggcg ccccgcccgg cgccggcgcc aaaccgttcg gatgcgacca
     1681 tgaaggaatc catcattgct gccggactca ccatcgaggg caagatcgaa ggcgcgggac
     1741 atgtccgcat tgccggcgac ttcaagggcg acgtcaacgt gcagggcaat ctcacgatcg
     1801 agcagggtgc gcacctgacc ggcggcgtga aggccgacac cgtggtcgtg ggtggacagc
     1861 tcgacggcaa catcgatgcc acgtcgcgcg tcgaactact gcagacgggc gtgttcaacg
     1921 gcgagctgaa ggccggctcc ctcaccgtcg cggccggttc acgcatgcgc ggcaaggcgg
     1981 agttcggttg ggacgagaag tccaagcact gaccgtcaaa tgaacggccg tgttgtggca
     2041 cccggcaaaa ccaggatctg tccgcactgc aagacgagca ttctggagag cgccagtgtt
     2101 tgtccgggtt gccaccatca tctgcgcttc gatcccaagg cgatcgctga aagccgcgca
     2161 caggcgactt tctccgcgtt gcgggtcgaa ggcgcattcc gcaatcccga gggcgcggag
     2221 tcctgggagt attcggtggt cgtctcgatt cgtaacgatc gcggcgagga agtcgcgcgc
     2281 aaggtagttg gcgtcggtgc gctcgatgcc cgggagagcc gcaccgtcac cctgtcagtc
     2341 gacgtgttcg cgccgcgcag cccgaagtag gcgccgcgtc cgccgcgaat tgtcagcgcg
     2401 acttgttagc cttgggtcgc agacggaatt ccctctccag ttcatcgact tcacgcttct
     2461 ggtgaaagtc cagccgtgcg cggggaagcg atgcgaagaa aagcagcgca gtacacgcct
     2521 cccgcgacgc accccacgag catggattcg aagttgaatc ggggatctgc cgtcgacgat
     2581 gggatccaat tcagcatgtg ggtgaatggc actgcgataa gggcgccgat caccccaccg
     2641 agaatcagcc ggatcgccat gaatccaacc cacgatgccg gaagtacgcg gcacgaagcc
     2701 agcaggagaa aggcgccgag aatgtacgga atggccgcga tcagcgtgac gaccaggaga
     2761 atgaacggca accaaagtag tccgtcggcg ctgccggagt ccgtcgaggt ccgggcctcg
     2821 ttggcgagga acgcgaaaaa tccaaccgcc gggccaaaga cggccgggcg atgaaagg
//
LOCUS       DATDEU010000491         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_531509, whole genome shotgun sequence.
ACCESSION   DATDEU010000491 DATDEU010000000
VERSION     DATDEU010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531509"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="hflK"
                     /locus_tag="VIV63_11725"
     CDS             complement(<1..882)
                     /gene="hflK"
                     /locus_tag="VIV63_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY6125310.1"
                     /translation="MAWNQPGNSNNNPWGKKPAPGGGDFDQAFKDWQKRIESLFGSGG
                     GRSSTPMLVLIIVGVLIWMLTGFYQVQPGNQGVVQRFGKHVETVGDGWGWRLPWPIES
                     VTKVDVAGVKSIAYKSRVLTAEPNMVELQVAVQYRITRAEDYLFRVREPIATLGEVSE
                     SAIREVVGRNDQQAILEAGRIKIAEDTRVIMQRTLDQYGAGIEVRSVNITDVQVPEAV
                     QQAQRDSVKAKADRERMIKEAQAYANGIIPVAQGNAARAVQEAEAYKSQVVSMATGDA
                     SRFSQLATAYEKAPNVTR"
     gene            complement(884..>2233)
                     /gene="hflX"
                     /locus_tag="VIV63_11730"
     CDS             complement(884..>2233)
                     /gene="hflX"
                     /locus_tag="VIV63_11730"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF008280.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HEY6125311.1"
                     /translation="SKLFERPRAGERAVLVRLGIGAPADPEDIQEFEALTRSAGAVPV
                     LSISGRRDRPDPRFFVGSGKADEIRIAAAEHNADVVLFDHPLSPSQERNLEKLIERRV
                     LDRSGLILDIFAQRARSHEGKLEVELAQLKHIASRLVRGWTHLERQRGGGIGNRGPGE
                     TQLETDRRLLGKRVKTLTARLEKIKQQRETGRQQRAEIPVPSLALVGYTNAGKSTLFR
                     ALTGADAYVADQLFATLDPTVRRIMLPGNTPVVVADTVGFIRELPHELVAAFQSTLTE
                     AREATLLLHVVDVSDPRRDEHIDEVNRVLAEIGAADLPQILVFNKIDRIDTPARVERD
                     ESGKVSHVWLSAAKSDGLDGLLQSVAERLARVARRATLRLPVSAGELRARLYAANLVQ
                     AERIGEDGAFEIDTELSEFELLKWAREPGVEVMTLSSDSPCTVAGAYLQSSPAFRSS"
BASE COUNT          346 a          709 c          740 g          438 t
ORIGIN      
        1 gcgcgtcacg ttcggcgctt tttcataagc cgtggccagc tgtgagaaac gcgaggcgtc
       61 gccggttgcc atcgagacga cctgcgattt gtacgcttcg gcttcctgca cggcgcgcgc
      121 cgcgttgccc tgcgcgaccg ggatgatgcc gttcgcgtaa gcctgggctt ccttgatcat
      181 gcgttcgcgg tcggccttgg ccttgaccga gtcgcgctgc gcctgctgca cggcctcggg
      241 gacctgcacg tcggtgatgt tcacgctgcg cacttcgatg ccggcgccgt actggtcgag
      301 cgtgcgctgc atgatgacgc gcgtgtcttc ggcgatcttg atgcggcccg cttcgaggat
      361 ggcctgctgg tcgttgcgcc ccaccacttc gcgaatggcg ctttcgctga cttcgccgag
      421 agtggcgatg ggttcgcgga ccctgaacaa atagtcttcg gcgcgcgtga tgcgatactg
      481 cacggccacc tgcagctcga ccatgttcgg ttcggcggtg agcacgcgtg acttgtaggc
      541 gatggatttg acgcccgcga cgtcgacctt ggtgaccgac tcgatgggcc acggcaaacg
      601 ccagccccag ccgtcgccga cggtttcgac atgtttgccg aagcgctgca cgacaccctg
      661 gttgcctggc tgcacctggt agaagcccgt gagcatccag atgagcacgc cgacgatgat
      721 gagcaccagc atgggagtgg acgaacgccc gccgccgctg ccgaacagcg actcgatgcg
      781 cttctgccag tccttgaagg cctgatcgaa atcgccgcca ccgggagcgg gtttcttgcc
      841 ccaggggttg ttgttcgaat taccgggttg gttccaggcc atatcagctg cttcggaagg
      901 cgggcgacga ttgtaggtag gcacctgcga ccgtacaagg tgagtcactc gagagcgtca
      961 taacttcgac gccgggctcg cgggcccatt tgagcagctc gaattcggat agttcagtgt
     1021 cgatctcaaa ggctccgtct tcgccgatac gttccgcctg gacgaggttc gcggcataca
     1081 gcctcgcccg gagctcgccg gccgataccg gcagccgcag cgttgcacgg cgcgccacgc
     1141 gcgccaggcg ctcggcgacg gactggagca gaccgtcgag gccgtcggac ttcgccgcgg
     1201 acagccagac gtggctcact ttgcctgatt cgtcgcgttc cacccgtgca ggcgtgtcga
     1261 tgcggtcgat tttgttgaac acaaggatct gcggcagatc cgcggcgccg atttcggcca
     1321 ggacccggtt cacttcgtcg atgtgttcgt cgcgccgcgg atcgctgacg tcgaccacgt
     1381 gcagcagcag cgtggcttcg cgcgcttcgg tcagcgtgga ctggaacgcc gccaccagtt
     1441 cgtgcggcag ctcgcggatg aatcctacgg tgtcggccac caccacgggt gtattgccgg
     1501 gaagcatgat ccttcgaacc gtgggatcga gcgtggcgaa caactggtcg gccacatagg
     1561 catccgcacc ggtcaacgcc cggaagagcg tcgacttgcc cgcgttcgtg tatcccacga
     1621 gcgccagcga aggcacgggg atctcggcgc gttgctggcg tccggtttcg cgttgctgtt
     1681 tgatcttttc gaggcgcgcg gtcagcgtct tcacgcgttt tcccagcaac cggcgatcgg
     1741 tttcgagctg ggtttcaccg ggaccacgat tgccgatgcc accgccgcgc tgacgctcga
     1801 ggtgggtcca gccgcgcacc aggcgcgagg cgatgtgttt gagctgcgcc agctccactt
     1861 cgagcttgcc ttcgtggctg cgcgcgcgct gcgcgaagat gtcgaggatc agtcccgagc
     1921 ggtcgagcac gcggcgctcg atgagcttct cgagattgcg ttcctgcgag ggcgacagcg
     1981 gatggtcgaa cagcaccacg tccgcgttgt gttcggcggc ggcgatccgg atttcatccg
     2041 ccttgccgct gccgacgaag aagcgcgggt ctggtcgatc ccggcgtccc gaaatgctga
     2101 gcacgggtac ggcgccagcc gatcgcgtga gcgcttcgaa ctcctggata tcttcggggt
     2161 cggccggcgc gcctatacca aggcggacga gcacggcccg ctcacctgcg cgtggtcgct
     2221 cgaacaattt gga
//
LOCUS       DATDEU010000492         6251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_92590, whole genome shotgun sequence.
ACCESSION   DATDEU010000492 DATDEU010000000
VERSION     DATDEU010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92590"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VIV63_11735"
     CDS             complement(<1..417)
                     /locus_tag="VIV63_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125312.1"
                     /translation="MNDAVLPSTDERVPRSTRPFINDRIQARTLHDVSRFIGADPVFI
                     DERIVQLQREWDIERTLEANAASMSLLGLGLGVFVDRRFLLLPAAVAVFLLQHALQGW
                     CPPLPMLRRLGIRTSAEIHNEIIALRILRGDFLDRVS"
     gene            complement(478..1614)
                     /locus_tag="VIV63_11740"
     CDS             complement(478..1614)
                     /locus_tag="VIV63_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125313.1"
                     /translation="MNTHIAHLQSEHKQTLLIVDDEPASMRALCDTLEFEGYKTYGFT
                     APAEALAAMRERSYDLLLADLQMPGTNGIDLMKSAQLIDPTLVAVIMTGHGALETAIA
                     AMKAGALDYIQKPIKLATTLPVLERALAVRQLRIEKKRLEESVRERTEELKIANRELE
                     AFSYSVSHDLRAPLRAVSVFTQSLLSDHVGGLSDEGRRLLQNVNAGAAHMDRLITDLL
                     RLSQLNRQALHKQPVRFGDLARRIIDEMVNERAGRDIEFVIADFPTWQVDPGLIQQVF
                     VNLISNAIKFTREREKARIEIGYRMDGTTLVCFVRDNGVGFNMKYMNKLFGVFQRLHS
                     ADQFEGTGVGLSIVRRIVERHGGKVWVDGEQDQGAAFFFSLPDC"
     gene            1980..4631
                     /locus_tag="VIV63_11745"
     CDS             1980..4631
                     /locus_tag="VIV63_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6125314.1"
                     /translation="MQPVRLVLIEDMPMEAEIAVRQLESNGFTCTWKRVDTESALRTV
                     LRELKPDVILSDFTLPGFDGLTALDISRQVAPDTPFIFLSGTLGEERAIDALQRGACD
                     YVLKTNMPRLVPAVRRALSDAAVRRGRVALEQQLRDIVSTSQDWIWEHDRSGKFTFSS
                     DSVRSTLGYSPEEILGTNASQYVHPEDLAAIDFAMHTLGPNQRTATNLQARWRHRNGS
                     YRWLERNMLVLLGDGGQVVGFRGSERDFSERRRQEKHISRLTRVLKMLSGVNSAVVRI
                     RQRREILAEACRLATSVGGYASAMVALIEPGTRTARPTAWSGSIDSQLPQHLTFSIGE
                     VAAEDTSVIGRVLRTGTSLVCNDLQSLDMTLAARASIMDSGFRSVVAYPLLVDRTPVG
                     AMMLTSYDVGAVGDEESRMLRELVANLSFALQYLHKEDEVRFLSYFDPLTGLAKRSLF
                     CERLARSLEPRIGRRGTPAVAVLDIEHLSTINDSFGRHAGDSLLQQVADRLKRHQDST
                     ELLAHFGGGTFGLIMEAAGDEDEAVHWMQEQLVEVFRAPFIVDGRGIPVDVKSGFARY
                     PDNGNDANALVQNAEAALRSAKSSGEKYLHHRLELSSAVVSRMTMEHRLRGAVERQEF
                     ELHYQTKIDVRTREVRGLEALVRWRDPEAGLVMPGAFLPLMETSGLIVPLGDWILRQA
                     AADLRRWQGAGLEAGRVAVNISPVQLRRRAFADHLLDLVGEWRNDNVGIDIEITEGVL
                     IDDVSSAVSQLRVLRRSGVRVAIDDFGTGYSSLSRLAELPVDMLKIDRSFVSGLTSSG
                     PGRTVAETIIALGRAFDMTTVAEGVETPEQFEMLAHMGCDQSQGYLHSRPLTATDIEP
                     LLRSSTVMPFHNGQMGNRELTSIARQG"
     gene            4737..6062
                     /locus_tag="VIV63_11750"
     CDS             4737..6062
                     /locus_tag="VIV63_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6125315.1"
                     /translation="MLKQLSRGRLIGLVAIVLVILFFVYRYMAGSGDAPEYQTATAER
                     GNVVSRVSTSGSLQAVVTVEVGSQVSGRIQELFADFNSPVKKGQKIAKIDPSLFGAAV
                     VQAEANVMAARANVTRLTITAEDAERQAKRASEVYDMRLISETERDNAVATARSARAG
                     VDQAQAQLAQSRAQLEQAQTNLRYTDIVSPTDGVVISRAVNVGQTVAASLSAPVIFTI
                     AQDLRKMEVHTNVAESDIGRLKPGMRVSFTVDAYPGEPFRGAIRDIRNAAQVVQNVVT
                     YDAVIDVANDDLKLKPGMTATVSIITDRRRDVLSVPNTALRFRPEGAAAGGAPATGTA
                     QAAGGQRRASAEAVQGQGRGEGEGSWRRKKEGDSDEDVAAPVVVKRTVYVLVDGKPVP
                     RDVVTGLTDGRITEVTGGELKEGEAVIVGVAGQNAQGQRGQQQRGPRIL"
     gene            6073..>6251
                     /locus_tag="VIV63_11755"
     CDS             6073..>6251
                     /locus_tag="VIV63_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6125316.1"
                     /translation="MALIELRNLGKIYQSGDTEFAALHDVNLSIERGEFVAITGSSGS
                     GKSTLMNLLGCLDSPS"
BASE COUNT         1136 a         2012 c         2002 g         1101 t
ORIGIN      
        1 actcacgcgg tccaggaaat cgccacgcag gatgcgcagc gcgatgatct cgttgtgaat
       61 ctccgcggaa gtccggatgc cgagccgtct caacatcggc aggggcgggc accagccttg
      121 caacgcgtgc tgaagcagga agaccgcgac agccgccgga agcagaagaa accggcgatc
      181 gacgaacacc ccgagcccca agcccaacag cgacatcgac gccgcattgg cttcgagagt
      241 gcgctcgatg tcccactcgc gctggagttg aacgatgcgc tcgtcgatga acaccgggtc
      301 ggcgccgatg aagcgcgaca catcgtgcag cgtccgggcc tgaatgcggt cgttgatgaa
      361 cgggcgggtg gagcgcggca cgcgctcatc ggtggaaggc aatactgcgt cattcatgac
      421 gccgatgatc gcccggcgcc aggggccggg actgtcagaa actgccgcag actacgttca
      481 gcaatccggc aggctgaaga agaacgcggc gccctggtcc tgttcgccgt cgacccacac
      541 cttgcccccg tggcgttcga caatccggcg gacgatggac agaccgacgc cggtgccttc
      601 gaactggtcg gcactgtgca ggcgctggaa tacgccaaac aacttgttca tgtacttcat
      661 gttgaagccc acgccgttgt cgcgcacgaa gcacacgagc gtggtgccgt ccatgcgata
      721 gccgatttcg atgcgggcct tctcgcgctc gcgcgtgaac ttgatcgcgt tggagatgag
      781 gttcacgaac acctgctgga tcaggccggg atcgacctgc caggtgggaa agtcggcgat
      841 cacgaactcg atgtcgcgtc ccgcgcgttc gttgaccatt tcatcgatga tgcgccgggc
      901 gagatcgccg aagcgaaccg gttgtttgtg cagcgcctgg cgattgagtt gcgacagccg
      961 gagcaggtcg gtgatgagcc ggtccatgtg cgccgcgccg gcattcacgt tctgcagcag
     1021 ccgccggcct tcatcgctca ggccgccgac gtgatcggac agcaacgact gcgtgaagac
     1081 cgacaccgcg cgcagcggcg cgcgcaggtc gtgtgaaacg gaatacgaaa aggcttcgag
     1141 ctcgcggttg gcgatcttca gttcttccgt gcgctcgcgg acgctttctt cgaggcgctt
     1201 tttctcgatg cgaagctggc gcacggccaa cgcgcgttcg agcaccggca gcgtggtcgc
     1261 gagcttgatg ggcttctgga tgtagtcgag cgcgccggcc ttcatggcgg cgatggcggt
     1321 ttccagtgcg ccgtggcccg tcatgatgac ggccaccagc gtgggatcga tgagctgcgc
     1381 ggacttcatg aggtcgatgc cgttggtacc gggcatctgc aggtcggcaa gcagcaggtc
     1441 gtagctgcgc tcgcgcatgg cggccagcgc ctcggcggga gcggtgaatc cgtaggtctt
     1501 gtaaccctcg aattcgaggg tgtcgcacag ggccctcatc gacgcgggct catcgtccac
     1561 gatgagcagg gtctgcttat gttcagactg gaggtgagct atgtgcgtgt tcacttctat
     1621 atgtacctgt atctgaaaat cgctcgcctg gcggtacgta ctttccgcgt aggtgattcc
     1681 ccgtttatcc cggtttcggc gcattttccg cctcctttag ccgagtgcca aatacctagg
     1741 gctaactctc agtttctgaa tgtcggacgt ttcccacacg gtttgggcgg gacgatgtcg
     1801 tctaactact tcacaatccc gttgtccggt ttgtgggttg cgtcacgtgc tcaataggag
     1861 taactggact aacttgcaat catctgcgtc gcacggggga agagccggcg cagcatgaaa
     1921 gcagcggttg ggtcagcact aggggtgctg gcgctcaacg aggcggggaa gaatacttaa
     1981 tgcaaccggt aagactggtt ctcatcgaag acatgccgat ggaagcggaa atcgctgtcc
     2041 gtcagctcga atccaatggg ttcacctgta cctggaaacg cgtggatacg gaatccgcgc
     2101 tccggacagt ccttcgcgag ctcaaacccg acgtcatcct ttccgatttc acgctgccgg
     2161 gcttcgacgg cctgacggcc ctggatatct cccgccaggt tgccccggat acccccttca
     2221 tcttcctgtc gggcacgctc ggcgaggaac gcgccatcga cgccctgcag cgcggcgcct
     2281 gtgattacgt actcaagacc aatatgccgc gcctggtgcc ggccgtccgg cgcgccctca
     2341 gcgacgctgc cgttcgccgt gggcgcgtgg cgctcgaaca gcagctgcgg gatatcgttt
     2401 ccacgtccca ggactggatc tgggagcacg accgttccgg caagttcacg ttcagcagcg
     2461 actccgtgcg cagcacgctc ggctattcgc ccgaagagat cctgggcacc aacgcttccc
     2521 agtacgtgca cccggaagat ctcgcggcca tcgacttcgc catgcacaca ctcgggccga
     2581 atcaacgcac ggccaccaat ctccaggccc gctggcgcca ccgcaacggc agctaccgct
     2641 ggctcgagcg caacatgctc gtcctgctgg gagacggagg gcaggtagtc ggttttcgcg
     2701 gcagcgagcg tgacttctcg gaacgccgcc gccaggaaaa acacatcagc cgcctgacgc
     2761 gcgtgttgaa aatgctcagc ggcgtcaaca gcgccgtggt gcgaatccgc cagcgccgcg
     2821 aaatcctcgc cgaagcctgc cggctggcca cctcggtggg cggctatgca agcgccatgg
     2881 tggcgctgat cgagcccggt acgcgtaccg cgcgtccgac cgcgtggtcg ggcagcatcg
     2941 acagccagtt accgcagcac ctgacgttca gcatcggcga ggtcgccgcc gaagacacga
     3001 gcgtcatcgg ccgcgtcctg cgcaccggaa cctcgctggt ctgcaatgac ctgcaatcgc
     3061 tggacatgac gctcgccgcg cgtgcgtcga tcatggattc tggattccgc agcgtggtgg
     3121 cctatccact gctcgtggac cgcaccccgg tgggcgcgat gatgctcacc tcgtacgacg
     3181 tgggcgccgt gggcgatgaa gagtcgcgca tgttgcgcga actcgtggcg aacctctcgt
     3241 tcgcgctcca gtaccttcac aaggaagacg aagtccgttt cctgtcttac ttcgatccgc
     3301 tcaccgggct cgccaagcgc agcctgttct gcgaacggct cgcgaggtcg ctggagccgc
     3361 gcatcgggcg gcgtggaacg ccggcggtgg cggtgctcga catcgaacat ctatctacca
     3421 tcaacgactc cttcggccgc cacgcgggcg actcgctgct gcagcaggtc gcggaccgcc
     3481 tcaagcgcca ccaggattcg accgaactgc tggcgcattt cggcggcggc accttcggtc
     3541 tcatcatgga agcggccggc gacgaagacg aggccgtgca ctggatgcag gagcaactgg
     3601 tcgaggtgtt ccgggcgccg ttcatcgtcg atggccgcgg aattcccgtc gacgtcaaat
     3661 ccggcttcgc gcgttaccct gacaacggca acgacgccaa cgcgctcgtg cagaacgcgg
     3721 aagccgcgct ccgcagcgcc aagagctcgg gcgagaaata tctgcaccat cgcctcgaac
     3781 tgagttcggc ggtggtgtcg cgcatgacca tggagcaccg cctgcgcggc gcggtcgagc
     3841 gccaggaatt cgagctgcat taccagacca agatcgacgt gcgcacgcgc gaagtccgcg
     3901 gtctcgaggc gctggtgcgc tggcgcgatc cggaggcagg cctcgtcatg cccggagcat
     3961 tcctgccgct gatggaaacc agtgggctca tcgtcccgct gggcgactgg attctgcggc
     4021 aggccgccgc ggatcttcgc cgctggcagg gcgccggcct cgaggcaggc cgcgtcgccg
     4081 tcaacatttc gcccgtgcag ctccgccgcc gggcctttgc cgatcacctg ctcgacctcg
     4141 tgggcgagtg gcggaatgac aatgtcggca tcgacatcga aatcaccgaa ggcgtcctga
     4201 tcgacgatgt cagctccgcg gtctcacaac tgcgagtgct gcgccggtcg ggcgtgcggg
     4261 tggccatcga tgacttcggt accggttatt catcgctgag ccggctggcc gaactgcccg
     4321 tggacatgct gaaaatcgac cgcagcttcg tcagcggcct cacatcgagc ggcccggggc
     4381 gcaccgtggc ggaaaccatc atcgcgctcg gcagggcctt cgatatgacc acggtggccg
     4441 agggcgtcga aacacccgag cagttcgaaa tgctggcgca catgggttgc gaccagtcgc
     4501 agggatatct gcacagccgg cccctgaccg cgaccgacat cgagcccttg ctgagaagct
     4561 cgacggtcat gccgtttcac aacggccaga tgggtaaccg cgagctaacg tccatcgccc
     4621 gtcaaggttg acagcctccc ataacttttt tgggatgact ctttagggga acgcagcggg
     4681 ccccgactga tctacactgg tgcgcgaaga gcgcgccagt cagatcggat acgaacttgc
     4741 tcaaacaact ttcccgcgga cgcctcattg ggctggtcgc gatcgtcctc gtcattctct
     4801 tcttcgtcta tcgatacatg gctgggagcg gcgatgcgcc ggagtaccag accgcgaccg
     4861 cggaacgagg caacgtcgtc tcgcgcgtca gcacctcggg cagcctgcag gccgtcgtca
     4921 ccgtcgaagt cggtagtcag gtgtcgggcc gcatccagga gctgttcgcc gacttcaatt
     4981 caccggtgaa gaagggtcag aagatcgcca agatcgatcc ttcgctgttc ggcgctgcgg
     5041 tggtccaggc cgaagccaat gtgatggcgg cgcgcgccaa cgtcacccgc ctcaccatca
     5101 ccgcggaaga tgccgagcgc caggcgaagc gcgcgtccga ggtgtacgac atgcgcctca
     5161 tctccgaaac cgaacgtgac aacgcggttg ccacggcccg gtccgcgcgt gccggcgtcg
     5221 accaggccca ggcgcagctc gcgcaatccc gcgcgcagct cgaacaggcg cagaccaacc
     5281 tgcgttacac cgacatcgtg tcgcccacgg atggcgtggt gatttcgcgc gcggtcaacg
     5341 tgggccagac ggttgcggcg tccctgtcgg cgcccgtcat cttcaccatc gcgcaggacc
     5401 tgcggaagat ggaagtgcat accaacgtcg ccgagtccga catcggccgg ctgaagccgg
     5461 gcatgcgcgt gagtttcacc gtggacgctt atccgggtga gccgttccgc ggtgccatcc
     5521 gcgacatccg caacgccgcc caggtggtgc agaacgtggt cacttacgac gccgtcatcg
     5581 atgtcgccaa tgacgacctc aaactcaagc ccggcatgac cgccaccgtg agcatcatca
     5641 ccgaccgtcg ccgcgatgtg ttgtccgtgc ctaacacggc gcttcgcttc cggccggagg
     5701 gtgctgccgc cggcggcgct ccggcgaccg gcaccgcgca agcggccggc gggcagcgcc
     5761 gcgcgagcgc tgaagccgtg cagggtcagg gccgaggcga gggcgaaggc agctggcgtc
     5821 gcaagaaaga aggcgactcc gatgaagacg tcgccgcgcc agtcgtcgtc aagcgcacgg
     5881 tctacgtgct ggtcgatgga aaaccggtgc cgcgcgatgt ggtcacgggg cttacggatg
     5941 gccgcatcac cgaagtcacc ggtggcgagc tcaaggaagg tgaggcggtc atcgtcggcg
     6001 tcgccggaca gaacgcccag ggtcagcgcg gtcagcagca gcgcggcccg cgcatcctct
     6061 agcagcgccg caatggccct catcgaactg cgcaacctcg gaaagatcta ccagtccggc
     6121 gatacggaat tcgccgcgct gcatgacgtg aacctgtcca tcgagcgcgg cgagttcgtc
     6181 gccatcacgg gttcgtcggg ttccggcaag tcgacattga tgaacctgtt gggctgcctc
     6241 gattcaccct c
//
LOCUS       DATDEU010000493         6432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1406236, whole genome shotgun sequence.
ACCESSION   DATDEU010000493 DATDEU010000000
VERSION     DATDEU010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406236"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2553
                     /locus_tag="VIV63_11760"
     CDS             <1..2553
                     /locus_tag="VIV63_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6125317.1"
                     /translation="SHATKRMILGQLPPQQRDASAFGQAESDLAEAMEEVHSAVVDAN
                     RPRLQPFWDAVLAQYEVVKAASPQSVSATEWFRVHEKLAELTLTLRDRVGVETGVILD
                     PGAETYPLVDAMFSRISSLETEVLRVNGFAHLAAAGDSTKEVSDGLAISAAKIDELSG
                     AIRSDLSDATYFSADGAEGYADVTPDGDATVNGMAALSSLVHSMRASDSGFDHAAIGL
                     GALKALEAVDELHDSGSPKLESLLRDRERKTRWSIGVQTASLILFMSLSTWLGVLVGR
                     GISRSLGVAVNTAENISAGKYDNVIDVRGDNETGRVLRAVGRMQEELKQRTESESTAR
                     DKERAVAAESMRVKVGLDSVSGNVLLADKDNRIIYLNKAIQQMFRACAADFRQQRPNF
                     DAHSLVGTSMDLQGSGAVDSRVAHQTQVKIGSRTFRVSTSPVVDGGGQWLGTVAEWID
                     RTQEVAVEEEIANIVVAASAGDLSMRIRKDGKDGFFATLADGMNGILTNMSTVVAEVN
                     AVVEAGKNQDLTARVDIAGKSGVFEQLSKGINGLMESMMAVVQQIKTAAQSVASGSDE
                     IARGNADLSGRTEQQASSLEETASSMEEMTSTVKHNADNASQANQLARAARQEAEQGG
                     QVVSNAVHAMQGINASSKKIADILGVIDEIAFQTNLLALNAAVEAARAGEQGRGFAVV
                     ASEVRTLAGRSATAAKEIKALIQESVARVEEGTKLVDKSGATLGDIVNAIKKVSDIVA
                     EISAASQEQSTGIEQVNKAIMQMDGLTQQNAALVEEAAAAAESLQDQARGLLDGMSRF
                     KVAAGTSAANETQRRAGTPRAVARAKAPREAQPKRAAPLRKAAGQDGEWSEF"
     gene            complement(2560..3492)
                     /gene="rsgA"
                     /locus_tag="VIV63_11765"
     CDS             complement(2560..3492)
                     /gene="rsgA"
                     /locus_tag="VIV63_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY6125318.1"
                     /translation="MSDAATADKFDAEVIAAFGRHLLVRSGDQELRARPAGRRLTIVC
                     GDRVRCEVDRAHDEVLAVEVLPRKTLLARANLRGESEPVVANITQLVVVVAPLPQPDF
                     FIVDRYLCAATAAGIKGAVAMNKSDLDANKIDPEDIEAYDKAGYARITCSAVDGQGLA
                     ELRALLSGAVSVLVGQSGVGKSSLVNALLPKQQIETGGLMREEEGRHTTTASRSYPLD
                     SGGTLIDSPGVRDFAPAIEQLDAETLGFPEVDARAGHCKFQDCRHMQEPACAVIAAVE
                     AGELSGRRYESYRRLRRRYTDLIEARGPAQRNRR"
     gene            3517..4074
                     /gene="orn"
                     /locus_tag="VIV63_11770"
     CDS             3517..4074
                     /gene="orn"
                     /locus_tag="VIV63_11770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002053300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY6125319.1"
                     /translation="MPSSDYLIWIDLEMTGLKPDTDVIIEMATIVTDVDLNIVAEGPV
                     IAIHQPPEVLAAMDEWNKRTHGASGLIERVRSSAYTMASAEKRTLETLTMLVEPGASP
                     MCGNSICQDRRFLARHMPTLEKFFHYRNLDVSTLKELARRWAPSIIPGFSKQGTHQAL
                     ADIQESIRELAYYREHFLAPEYQKL"
     gene            4071..5036
                     /locus_tag="VIV63_11775"
     CDS             4071..5036
                     /locus_tag="VIV63_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY6125320.1"
                     /translation="MKDGLPGFADVVAAAARIGPHVVRTPVMRSAAFDARVNAQVFFK
                     CENLQTGGAFKYRGAMNVLLQLDPARTPVVATHSSGNHGNALALAARARGMRAIVVVP
                     RDSARSKIANIEAAGARVELCEPGLPAREKRLAEILAHEPGELVHPFDDERIIAGQGT
                     AALELLAEIPSLDVIGAPIGGGGLIGGTALAAKGTVPGIRVIGAEPEMADDAFQSFRS
                     GERRAVIAPKTMADGLRGSIGVRNFELLRRLVDDVVTVSEAQIVEAMRIVLADFKLLV
                     EPSSAVAVAAVLSGKLGRAGERIGLVISGGNIDLDLCPFLRGERL"
     gene            5123..5764
                     /locus_tag="VIV63_11780"
     CDS             5123..5764
                     /locus_tag="VIV63_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125321.1"
                     /translation="MTTLHRRSVLLLCLAALGAAGMFLGPDGWFGIDIGPVGSVVLYA
                     ALWLFLIHLSRNPEGALPADASLAERLAWVSAMFSTLLWFHWLNYLIALRSLGAEADQ
                     LWNPASRAFGVNLGLILVAWIVVTIVLRSGNRETVEADERDLRIQNEAGRAGSAFMAV
                     LIIGLIAALATQPERLAPWLRPLIVANVLVGLLVARALTENVYSVIRYRIQHA"
     gene            5761..5976
                     /locus_tag="VIV63_11785"
     CDS             5761..5976
                     /locus_tag="VIV63_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6125322.1"
                     /translation="MSETAVRNEIRRWRQENGAMTQAELARLCGVTRQTIIALEAEKY
                     SPSLELAFRLARALGRNLEEVFRFEEK"
     gene            6060..>6432
                     /locus_tag="VIV63_11790"
     CDS             6060..>6432
                     /locus_tag="VIV63_11790"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HEY6125323.1"
                     /translation="MLSDALSIVSWRSRPRSFVALMSLYESNYLRLAQLAGDLKALAD
                     VRVSRVGQDCDLRLTVSERSPYTTSFDLTYLFEQAEGLTTYPDMRVRVYHDARLVEAQ
                     EWASQHEHEGLRRLRGVAEREL"
BASE COUNT         1169 a         2012 c         2137 g         1114 t
ORIGIN      
        1 tcgcacgcca cgaagcgcat gattctcggc cagttgccgc cgcaacagcg cgatgccagt
       61 gccttcgggc aggcggagtc agatctcgcc gaggcgatgg aggaagtgca ttccgcggtg
      121 gtcgatgcga accggccacg cctccagccc ttctgggacg cagtgctggc gcaatacgaa
      181 gtcgtgaagg ccgcgagccc gcagagcgtg tcggcgacgg aatggtttcg cgtgcacgag
      241 aaactggccg aactcaccct gacattgcgt gaccgggtcg gtgtggaaac cggcgtcatc
      301 ctcgatcccg gcgcagaaac ctatccactc gtcgacgcga tgttcagccg gatctcgagc
      361 ctcgagaccg aggtgcttcg ggtcaacggt ttcgcgcatc tcgcggccgc tggcgattcg
      421 acgaaagaag tttccgatgg gctggccatt tcagcggcca agatcgacga actgtcgggc
      481 gcgatccgct cggatctgag tgatgccacg tacttctcgg ccgatggcgc cgaaggttat
      541 gcggatgtca cccccgacgg tgacgcgacc gtgaatggca tggccgcgct ttcgagcctg
      601 gttcattcca tgcgcgcttc ggattccggt tttgaccatg ctgcgatcgg cctcggggcc
      661 ctcaaggcgc tcgaggccgt cgatgaattg cacgactcag gcagccccaa actcgaatca
      721 ctgctgcggg atcgcgagcg caagaccaga tggtcgatcg gcgtgcaaac cgcttcgttg
      781 atcctgttca tgtcgctctc gacgtggctc ggtgtgctcg tgggtcgcgg catttcccgc
      841 tcgctgggcg tcgcggtcaa caccgccgaa aacatctcgg cgggcaaata cgacaacgtg
      901 atcgacgtgc gcggcgacaa cgaaactggc cgtgtgctgc gcgcggtcgg tcgcatgcag
      961 gaagagctga agcagcgcac ggaaagcgaa agcaccgcgc gcgacaagga acgcgccgtg
     1021 gcggccgaga gcatgcgcgt gaaggtgggt ctcgacagcg tgtcgggcaa cgtgctgctg
     1081 gcggacaagg acaaccgcat catctatctc aacaaggcga tccagcagat gtttcgtgcc
     1141 tgcgcggcgg acttccgcca gcaacgtccg aatttcgatg cccattcgct cgtcggcacg
     1201 tcgatggatc tgcagggttc cggcgccgtc gattcgcgcg tcgcgcacca gacgcaggtc
     1261 aagatcggca gccgtacatt ccgggtcagc accagcccgg tggtcgatgg cggtggtcag
     1321 tggctgggca ccgtggccga gtggatagat cgcacgcagg aagtggcggt ggaagaggag
     1381 atcgcaaaca tcgtcgtcgc tgcttcggct ggcgacctgt cgatgcgcat ccgcaaggac
     1441 ggcaaggacg gcttcttcgc gacgctcgcc gacggcatga acggcatcct caccaacatg
     1501 tcgacggtgg ttgccgaggt caacgccgtc gtcgaggctg gcaagaatca ggatctgacc
     1561 gcgcgcgtcg acatcgccgg caagtccgga gtcttcgagc aattgtcgaa gggcatcaat
     1621 gggctcatgg agtcgatgat ggccgtcgtg cagcagatca agaccgcggc gcagtcggtg
     1681 gcgtcgggtt ccgacgagat cgcgcgcggc aatgcggatc tcagcgggcg taccgagcag
     1741 caggcatcgt cgctggaaga aaccgcgtcc agcatggaag agatgaccag caccgtgaaa
     1801 cacaacgcgg acaatgcctc gcaggccaac cagctcgcgc gcgccgcgcg ccaggaagcg
     1861 gagcagggtg gccaggtggt gtcgaacgcg gtgcatgcca tgcagggcat caacgcgtcg
     1921 agcaagaaga ttgccgacat cctgggcgtc atcgacgaga tcgcattcca gaccaatctg
     1981 ctggcgttga acgcggcggt ggaagccgct cgcgcgggcg agcagggacg cggattcgcc
     2041 gtggtcgcga gcgaagtgcg cacgctcgcg ggacgcagcg ccaccgcagc caaggaaatc
     2101 aaggcgctga tccaggagag cgtcgcgcga gtcgaggaag gaacgaagct ggtcgacaag
     2161 tccggcgcga cgctgggcga catcgtcaat gccatcaaga aggtatcgga catcgttgcg
     2221 gagatcagcg cggcgagcca ggaacagtcc accggcatcg agcaggtgaa caaggccatc
     2281 atgcagatgg atgggctgac gcagcagaac gcggcgctgg tcgaagaagc ggccgcggcc
     2341 gccgagtccc ttcaggacca ggcgcgcggg ctgctcgacg gaatgtcccg attcaaggtc
     2401 gccgcgggaa cgtccgcggc caacgaaaca cagcggcgtg cgggtacgcc gcgcgcggtg
     2461 gccagagcga aggcgccgcg cgaggcccag ccgaagaggg ctgcgccgct gcgcaaggcc
     2521 gccggccagg acggcgagtg gagcgagttc taacgcgaac tatcggcgat tgcgttgtgc
     2581 cggcccgcga gcttcgatga ggtcggtata acgccggcgc agccggcggt aactctcgta
     2641 tcgtcgcccc gacaattcgc cggcttccac ggcggcgatc accgcgcaag cgggttcctg
     2701 catgtgccgg caatcctgga atttgcagtg ccccgcgcgc gcgtcgactt ccggaaaacc
     2761 gagagtctcg gcatccagct gctcgatggc cggcgcaaag tcgcgcacgc ccggagagtc
     2821 gatgagcgtg ccaccggaat cgagcggata ggatcgcgac gcggtggtgg tgtgtcggcc
     2881 ttcctcctcg cgcatcagtc cgccggtttc gatctgttgc ttcggcaaga gcgcgttcac
     2941 gagcgatgac ttgccgacgc ccgactgccc gaccagtacg ctgacggcgc ccgagagcag
     3001 cgcgcgcagc tcggcgagac cctggccgtc gacggccgaa cacgtgatcc gggcgtaacc
     3061 cgccttgtcg taggcctcga tgtcttccgg gtcgatcttg ttagcgtcga ggtcggactt
     3121 gttcatggcg accgcgccct tgattccggc ggcggtcgcc gcgcacaggt agcgatcgac
     3181 gatgaagaag tcgggctgcg ggagcggcgc cacgaccacg acaagctggg tgatgttggc
     3241 gaccacgggt tcgctttcgc cgcgcagatt ggcgcgcgcc agcagcgtct tgcgcggcag
     3301 cacttcgacg gccagcactt catcgtgcgc gcggtcgact tcgcatcgga cgcgatcgcc
     3361 gcagacgatg gtgaggcgcc ggcccgcggg gcgggcgcgc aactcctggt caccgctgcg
     3421 caccagcagg tggcgaccga aagccgcgat gacttcggca tcgaatttgt cggcggtggc
     3481 ggcatcggac attcgtgtag tatcgcgtca ttctttatgc cgagcagcga ctatctcatc
     3541 tggatagatc tcgagatgac gggcctcaag cccgacaccg acgtgatcat cgagatggcc
     3601 accatcgtca ccgacgttga tctcaacatc gtcgccgaag gaccggtcat cgccattcac
     3661 cagcctcccg aagtgctggc cgccatggac gaatggaaca agcgcacgca cggcgcttcc
     3721 ggactgatcg agcgcgttcg gtccagcgcc tacacgatgg ccagtgcgga gaagcgcaca
     3781 ctcgaaaccc tgacgatgct ggtggaaccg ggtgcctcgc cgatgtgtgg caacagcatc
     3841 tgccaggacc ggcgtttcct cgcgcgccac atgcccacgc tcgagaagtt tttccactac
     3901 cgcaatctcg acgtgagcac gctcaaggaa ctggcgcgcc gctgggcgcc gtcgatcatc
     3961 ccgggcttct ccaagcaggg aacgcaccag gcgctggccg acatccagga atccatccgc
     4021 gagctcgcgt actaccgcga acatttcctc gcgcccgaat accagaagtt atgaaagacg
     4081 ggctgccggg attcgcagac gtcgtggcgg cagccgcgcg tatcggcccg cacgtggtgc
     4141 gcacgcccgt catgcgttcg gccgcgttcg atgcccgcgt gaacgcccag gtcttcttca
     4201 agtgcgagaa cctgcagacg ggcggggcgt tcaagtatcg cggcgccatg aacgtgttgt
     4261 tgcagctcga ccctgcgcgc actccggtgg tggcgacgca ttcgtcgggc aaccacggca
     4321 acgcgctcgc gctcgcggca cgcgcgcgcg gcatgcgcgc gatagtcgtc gttccccggg
     4381 attccgcgcg ttcgaagatt gccaacatcg aagccgcggg cgcgcgcgtg gagttgtgcg
     4441 agcccggact gccggcacgc gaaaagcggc tcgcggaaat tctcgcccac gaacccggcg
     4501 agctcgtcca tccgttcgat gacgaacgaa tcattgccgg gcagggcacg gcggcgctcg
     4561 agctgctcgc cgaaattccc tcgctcgatg tcatcggcgc gcccatcggc ggcggcggac
     4621 tgattggtgg taccgcgctc gcggccaaag gaaccgtgcc aggcattcgc gtgattggcg
     4681 ccgagcctga gatggccgac gatgcgtttc aatccttccg cagcggcgaa cgtcgtgcag
     4741 tgatagcgcc gaagaccatg gccgatggtt tgcgcggctc gatcggcgtg cgcaatttcg
     4801 aactgctgcg gcggctcgtc gacgatgtgg tcacggtcag cgaggcgcag atcgtcgagg
     4861 cgatgcgcat cgtgctcgcg gatttcaaac tgctcgtcga accctcctcg gcggtcgccg
     4921 tcgcggcggt cctctcgggc aagctcgggc gcgccggcga acgcatcggt ctcgtgattt
     4981 ccggcgggaa catcgacctc gatctgtgcc cgttcctgcg gggcgagcgc ctataagaag
     5041 agtcgggaat gtaaagaata gttgacatct gcgtatcgag atgtaaggta taccggacat
     5101 tcaccacgcc gggatacccg ccatgaccac tcttcaccgc cgcagcgtct tgctgctctg
     5161 cctcgccgca ttaggcgcgg ctggcatgtt tctgggtccc gatggctggt tcggcatcga
     5221 tatcgggccc gtcggctctg tggtcctgta cgccgcgttg tggctattcc tcattcacct
     5281 gtcgaggaac ccggaagggg cgctccccgc cgacgcgtcg ctcgccgaac gcctcgcctg
     5341 ggtgtcggcg atgttctcca cgctcctctg gttccactgg ctgaattacc tcatcgccct
     5401 gcgctcgctc ggtgccgaag cagaccagtt gtggaacccg gcgtcgcgcg cctttggcgt
     5461 gaatctgggt ctgatcctcg tcgcctggat cgtcgtaacg atcgtgctgc gctcggggaa
     5521 tcgcgaaacc gtggaggccg atgaacgcga tctgcgcatt cagaacgaag cgggcagggc
     5581 tggcagcgcc ttcatggcag tgctgatcat cgggttgata gcggcactgg cgactcagcc
     5641 cgagcggctt gcgccctggc tccggccgtt gatcgttgcg aatgtgctgg tggggctgct
     5701 cgtggcgagg gcactcacgg agaacgtgta cagcgtcatc cgataccgga ttcaacacgc
     5761 atgagcgaga ccgcggtacg caacgagatc cggcgctggc gccaggagaa cggcgcgatg
     5821 acgcaagcgg aactcgcgcg tctctgcggc gtcacacgtc agacgatcat agcgcttgaa
     5881 gcggagaaat attcaccgtc gctcgagctc gcattccggc tggcgcgcgc tctcggccgc
     5941 aacctcgaag aggttttccg gttcgaggaa aaataaatct caccgcgcga tcctcatctg
     6001 acgattggct caacttttgc gtcttgaatg atcgcgttgg tccgctatat tcggactcaa
     6061 tgttaagtga cgcgttatcc atcgtttcct ggcgctcgcg cccgcgcagt ttcgtcgcgc
     6121 tcatgtcgct gtacgaaagc aactacctgc ggctcgcgca gctcgccggc gatctcaagg
     6181 cgttggccga cgtgcgggtg tcgcgcgtgg gccaggactg tgatttgcgt ctcaccgttt
     6241 ccgagcgttc gccgtacacc acgagcttcg atctcaccta tctgttcgag caggccgaag
     6301 gcctgactac ataccccgac atgcgcgtgc gcgtatatca cgacgccagg ctggtggaag
     6361 cccaggaatg ggcgtcgcag cacgaacacg aggggctgcg ccgcctgcgg ggcgtggcgg
     6421 agcgtgaact gg
//
LOCUS       DATDEU010000494        10719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1989605, whole genome shotgun sequence.
ACCESSION   DATDEU010000494 DATDEU010000000
VERSION     DATDEU010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989605"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VIV63_11795"
     CDS             complement(<1..282)
                     /locus_tag="VIV63_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY6125324.1"
                     /translation="MKKTTLVNHPPDVSPPPGNLPVIAPIYQSVKYEFETVDDTLRML
                     RGEAPGYFYMRASNPTTRQLELTLAQLQGRDDCLATASGVNAIAQTLIGL"
     gene            complement(279..1058)
                     /locus_tag="VIV63_11800"
     CDS             complement(279..1058)
                     /locus_tag="VIV63_11800"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY6125325.1"
                     /translation="MSFRVSIAQQPLVWQDAAANRAHFEKVLAPLAGATDLVVLPEMF
                     TTGFTMKPEEQAEDAAGATRQWLLAQARTLDAAVGGSVAVNDDGRYYNRFMLAMPDGL
                     TYWYDKRHLFRMGGEHRHYSAGSHALIVEWRGARLCPLVCYDLRFPVWSRRRPELEYD
                     VVIYAANWPAARRFAWTSLLRARAIENQAYCVGVNRVGDDGAGIAHCGDSVVLDFTGQ
                     PLLELNDHAQVITVPLDLAGLRNWRDKFPAHLDADAFTLES"
     gene            complement(1055..2251)
                     /locus_tag="VIV63_11805"
     CDS             complement(1055..2251)
                     /locus_tag="VIV63_11805"
                     /inference="COORDINATES: protein motif:HMM:NF006569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEY6125326.1"
                     /translation="MSFPEGFQSKLPDVGTTIFTVMSRRAAEQGAVNVGQGFPDYPID
                     PSLAACVTDAISAGFNQYAPMEGSVALRAAIAAKLSAAGGRAVDPETELTVTCGGAES
                     IYSAIQAVVGPGTEAIVFDPAYDAYDPAVRLAGGRCIHIPLAAPDFRYDWDRVRSAIN
                     DRTRLILINNPHNPACTVASAADLDALAALTRDRPIAVLGDEVYEHVQYDGRVHQSVM
                     NHPELKSRSFAVYSFGKSLHITGWRVGYCVAPPTLTREMRKVHQFNAFSTPAPLQQAI
                     ALYLQRYPDAWRALAAFFSAKRDLLRQGLAGSGLVLPPAAGTYFQLADYSSLGGGLAE
                     CNDVQFTERLINEAKVAVIPLSPFYREPPAGMRIVRLCVAKRDETLTEAAARISAYTQ
                     SNREKS"
     gene            complement(2306..2542)
                     /locus_tag="VIV63_11810"
     CDS             complement(2306..2542)
                     /locus_tag="VIV63_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125327.1"
                     /translation="MAKSARKHNAPWTPADLKKMRQLAKQKLSARLAAKELGRSTGAV
                     KYKAMVEGIRFRFIDQPAGVQLKLARKAKRAKKK"
     gene            complement(2589..3443)
                     /locus_tag="VIV63_11815"
     CDS             complement(2589..3443)
                     /locus_tag="VIV63_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6125328.1"
                     /translation="MRTQGLFSKTMLWVGLGFLYIPILSMVFFSFNNSRLVTVWDAAH
                     SPTVKWYVQLFKDHQILSAAWLSIQVAASTATGAVILGTLAGLVLSRFGPFRGRAFLQ
                     GLTTAPLVMPEVITGLSMLLLFVSLEQLLQFMFGWQFDRGFATITIAHITFTMAYVTV
                     VVQSRLAGFDDSLEEAALDLGARPAKVFFRITVPLILPAILSGWLLAFTLSWDDLVVT
                     QFVSGPGSNTLPMVIFSRVRLGVSPVVNALATIMVLIVALGVILSAVLMRRQEQRRKR
                     EEQMAATG"
     gene            complement(3440..4447)
                     /locus_tag="VIV63_11820"
     CDS             complement(3440..4447)
                     /locus_tag="VIV63_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6125329.1"
                     /translation="MKPAARTLGSRDSYGRLGPLWTFLLRYGPPRWSEYVVQNWTGQR
                     LVIAIPYLWLIVFFLIPFLIVLKISFAEFSPLGRPPYEPVFSWLEEGALQIKLLIGSY
                     VYLFNEPLYVSAWIYSIKVAFFSTVFCLALGYPMAYAISRSPPTQRNIFLLLIILPFW
                     TSFLLRVYAWVGLLKTDGLINQVLFKWLGLVDEPLAMMNTSFAVYIGIVYSYLPFMIL
                     PLYSNLEKHDNTLLEAAQDLGAGPIRSFLRVTLPLSFPGIVAGSLLVFIPAVGEYVIP
                     SLLGRTDQLMVAKLLSDEFFLNRDWPRASAVAIAMLLLLVVPIMIFQRFQNKELQAQK
                     K"
     gene            complement(4470..5555)
                     /gene="potA"
                     /locus_tag="VIV63_11825"
     CDS             complement(4470..5555)
                     /gene="potA"
                     /locus_tag="VIV63_11825"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEY6125330.1"
                     /translation="MRIENVTKRFGDFVAVNNVTLKIFKGEIFCLLGGSGCGKSTLLR
                     MMAGFENPSSGSIFLDGQDMAGVPPYERPVNMMFQSYALFPHMTVEKNIAFGLEQEKG
                     LSRADIATRVTDILSIVKLDQFAARRPHQLSGGQRQRVALARALVKRPKLLLLDEPLG
                     ALDKKLREATQFELINIQEQLGVTFIVVTHDQEESMTLASRIGVMNRGEIVQIGTPTE
                     IYEFPQNKFVADFIGSVNMFEGTMVEDLPDRVRIKSDELGGIIYVDHGISSAPGAIVY
                     AAIRPEKINILRAQPAETHENCVKGVVKEIAYMGDVSIYLVKIDTGKIVRVTLPNVER
                     MSDDERILWDETVWLTGHGSSPVVVTQ"
     gene            complement(5691..6845)
                     /locus_tag="VIV63_11830"
     CDS             complement(5691..6845)
                     /locus_tag="VIV63_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6125331.1"
                     /translation="MSTRSLSALVSASCIFLLVACGKKEEAPPPAAAAPATPAYDTDA
                     EKELNVYNWSDYIEPSVLDEFTKETGIKVNYEVMDSNELLETKLVAGRTGYDVVVPSA
                     NFLARQIKSGIYQKLDKSKLDNLKNLDPDITKRLEVFDPGNEHAVNYMWGTSGVAYNE
                     EAIKAAMPDAPVDSFAMFWDPKVVSKFAKCGVSVLDAPSEIIGTVLIYLGKDANSESP
                     EDLKAAEKVLLSARPYIRLINSSKYIEDLANGEICLALGWSGDTLQARDRAAEAQKPF
                     TIKYNIPKEGAVMFFDSMAIPADASHVKNAHVFINYMLRPEVAAKNSNFISFANSNAA
                     SWPLVTAEVKNNPGIFPTPEMMPKLVPDLPESAEFTRTLTRTWTRFRTGK"
     gene            complement(6924..8312)
                     /locus_tag="VIV63_11835"
     CDS             complement(6924..8312)
                     /locus_tag="VIV63_11835"
                     /inference="COORDINATES: protein motif:HMM:NF005682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY6125332.1"
                     /translation="MSANPTDTSGWQRLDSAHYLHPFTDHKQLAAKGARIVTRAEGVY
                     IYTSEGDKILDGMSGLWCVALGYGRKELSDAAYKQLLELPYYNSFFQSTTTPAIELAR
                     LLKEVTPPQFNHVFYTGSGSEANDTLIRLVRRYWQLLDQPERNVIVSRWNGYHGSTVA
                     GASLGGMKPMHEQLGPNGALPGIVHINQPYWFGEGGDLSPAEFGLKCARELESKILEV
                     GPERIAAFIGEPVQGAGGLVIPPDTYWPEIQRIVDKYGILLASDEVICGFGRTGQWFG
                     CEHYGTRPDFMTMAKALSSGYLPIGGLMVGDRVANVLIDKGGEFFHGFTYSGHPAACA
                     VAIANLEIFRRERIVERVREEAAPYLAARWSELASHPLVGEARCTGLLAAIELVPEKH
                     SRRFFEPRGEVGLRMRDNCTRNGLVMRATRDTLFIAPPLVISRSEIDELLSKVVRSLD
                     ETATQARAEGWL"
     gene            complement(8329..9669)
                     /locus_tag="VIV63_11840"
     CDS             complement(8329..9669)
                     /locus_tag="VIV63_11840"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY6125333.1"
                     /translation="MANEIRTFLRDHGISEVEAIIPDMAGIARGKIMPAEKFAEDEGM
                     RLPESIFLQTVTGDYPPDTDKAMSPAEIDIILKADPKTVRRVPWAAEPTAQVIHDCFY
                     SDGRRVGMAPRGVLRHILEMYANRGWEPVVAPELEFYLVEPNIDADYPLKPPVGRSGR
                     AEPGRQSYSIAAVNEFDPLFDDMYQFCEDQDIEIDTLIHEDGAAQMEINLLHGNALDL
                     ADQAFLFKRTAREAALRHKMYATFMAKPHAKEPGSAMHIHQSVVDTKTRKNIFSNSDG
                     TPTPLFFSHIAGMQKYLPAAMALLAPNVNSYRRISRFQLAPINVQWGYDNRTAGLRVP
                     ASDPEARRVENRLAGADANPYIAIATSLACGYLGMVEGLQPSEPISGSAHELPFSLPR
                     SLDDAIRELRECEPLVRILGDPFISAYTIVKEAEYEVFLQVISSWEREHLLLNV"
     gene            complement(9675..10442)
                     /locus_tag="VIV63_11845"
     CDS             complement(9675..10442)
                     /locus_tag="VIV63_11845"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY6125334.1"
                     /translation="MASSLEKKPVIGIPADRRMIGPHPFHAVGEKYIAAIVQAADCLP
                     LLIPVLESPLDVTELLSRVDGIFFSGSPSNVEPVRYAGPASVPGTLHDPHRDETTLSL
                     IPRAIEQSVPVFGVCRGFQEMNVAMGGTLHQRVHEVMGLDDHREDKEQPLEVQYGPAH
                     DVVLEPGSVLRGIAGQERIRVNSLHWQGVDKLGPGLAVEARATDGLVEAFRVASAPAF
                     ALAVQWHPEWQVMSNPFSKALFAEFGKAARTKAKVVR"
BASE COUNT         1967 a         3222 c         3522 g         2008 t
ORIGIN      
        1 gagaccgatg agtgtctgcg cgatggcgtt cacgcccgac gcggtagcga ggcagtcgtc
       61 gcggccctgg agctgcgcca gcgtcagctc gagctggcgc gtggtggggt tgctcgcccg
      121 catgtagaaa taaccgggcg cttcgccgcg cagcatgcgc agggtgtcgt cgacggtttc
      181 gaattcgtac ttgacgctct ggtagatagg cgcgatcacc ggcaggttgc cgggcggcgg
      241 tgagacatcg ggcggatgat tgacgagcgt ggttttcttc atgattccag cgtaaaggca
      301 tcggcatcga gatgtgcggg aaatttgtcg cgccagttgc gcagacccgc gaggtcgagc
      361 ggcacggtga tcacctgcgc atgatcgttg agctcgagca gcggctgacc ggtgaagtcg
      421 agcacgacgc tgtcgccgca atgggcgatg cccgcgccat catcgccgac acggttcacg
      481 ccgacgcaat aggcctggtt ttcgatggca cgtgcgcgca gcaacgaggt ccacgcgaag
      541 cgccgtgccg ccggccagtt ggcggcgtag atgaccacgt cgtactcgag ctcgggccgg
      601 cgccggctcc acaccgggaa gcgcaggtcg taacagacca gcggacacaa ccgcgccccg
      661 cgccattcga cgatgagcgc gtggcttccg gcgctgtagt ggcgatgctc gccgcccatg
      721 cggaacaggt gccgcttgtc gtaccaataa gtgaggccgt ccggcatcgc gagcatgaag
      781 cggttgtaat accggccgtc gtcgttgacc gccacgctgc cgcccacggc cgcgtcgagc
      841 gtgcgcgcct gcgccagcag ccattgccgg gtagcgccgg cggcatcctc ggcctgttct
      901 tcgggcttca tcgtgaagcc ggtggtgaac atctcgggca agactacgag atcggtggcg
      961 ccggccagtg gcgccagcac tttctcgaaa tgcgcgcgat tggccgccgc gtcctgccag
     1021 accagcggtt gctgcgcgat gctgactcga aaactcatga cttctctctg ttcgactgag
     1081 tgtacgcgct gatacgcgcg gcggcttcgg tcagcgtttc atcgcgcttg gcgacgcaca
     1141 gccgcacgat gcgcatgccc gcgggcggct cgcggtagaa cggcgacagc gggatgacgg
     1201 ccacctttgc ctcgttgatg agccgctcgg tgaactgcac gtcgttgcat tcagcgagcc
     1261 cgccacccag cgatgaatag tcggccagct ggaaatacgt gccggcggcc ggcggcaaca
     1321 cgagcccact gccggcaagg ccctggcgca ggaggtcacg cttggcgctg aagaaggccg
     1381 cgagcgcgcg ccacgcgtcc gggtagcgct gtagatagag cgcgatcgcc tgttgaagcg
     1441 gcgcgggcgt gctgaacgcg ttgaactggt ggaccttgcg catctcgcgg gtcagcgtgg
     1501 gcggcgccac gcaatagccc acccgccaac cggtgatgtg cagcgacttg ccgaaggaat
     1561 agaccgcgaa actgcgcgac ttcagttccg ggtggttcat gaccgattga tgcactcggc
     1621 cgtcgtactg cacgtgctcg tagacctcgt cgccgagcac ggcaatgggc cggtcgcgcg
     1681 tgagcgccgc cagggcatcg aggtccgcgg ccgaggccac ggtgcaggcc ggattgtgcg
     1741 gattgttgat gaggatgagg cgggtgcgat cgttgatcgc gctgcggacc cgatcccagt
     1801 cgtagcggaa gtcgggcgcc gcgagtggaa tgtggatgca gcggcctccc gcgaggcgca
     1861 ccgccggatc ataggcgtcg taggcggggt cgaagacgat ggcctcggtc ccggggccga
     1921 ccaccgcctg gatggcggag tagatagatt ccgcgccgcc gcaggtgacg gtgagctcgg
     1981 tttcggggtc gaccgctcgg cccccggcgg cagaaagctt ggcggcaatg gcggcgcgca
     2041 gcgcaacact gccttccatc ggggcgtact ggttgaagcc cgcggatatg gcgtcggtga
     2101 cgcaggcggc gaggctggga tctatggggt agtcaggaaa tccctgtcct acattgaccg
     2161 cgccctgttc ggcggcgcgg cgcgacatca cggtgaatat cgtggtgccg acgtcgggca
     2221 gtttgctctg gaacccttcc ggaaaactca tcggcttgaa tgaaagccgc cgcgcacccc
     2281 cgtacgcggc gtcttcgtcc gacgcctact tcttcttggc gcgcttggcc ttgcgggcaa
     2341 gcttcaactg aacgccagcg ggctgatcga tgaaacggaa gcggatgcct tccaccatcg
     2401 ccttgtactt gaccgcgccg gtggaccggc ccagttcttt ggcggcgagc ctggcggaca
     2461 gtttctgttt ggcaagctgc cgcatcttct tgaggtctgc gggcgtccag ggagcattgt
     2521 gtttacgggc tgatttggcc attgatcgga tcctcgtcaa attgtcgatg gtggaagtct
     2581 agcagtagtt agccggttgc ggccatctgt tcctcgcgtt tgcgacgttg ttcctgccgg
     2641 cgcatgagaa ccgccgacag gatcacgccg agagccacta tcagtaccat tatggttgct
     2701 agtgcgttca cgaccgggct tacacctagt cgaacccgag aaaatatgac catgggcaac
     2761 gtgttggacc cagggccgga gacgaactgc gtcacgacga ggtcgtccca ggaaagagtg
     2821 aaggccagca gccatcccga caggatggcg ggcaggatga ggggcacggt aatgcggaaa
     2881 aacaccttcg cgggccgggc gcccaggtcg agcgccgctt cttccagcga gtcgtcgaag
     2941 cccgccaggc gcgactggac caccacggtg acgtacgcca tggtgaacgt gatgtgcgcg
     3001 atggtgatag tggcgaagcc gcggtcgaac tgccagccaa acatgaactg caggagctgc
     3061 tcgagcgaca cgaacagcag cagcatggac aggccggtga tgacttcggg catgaccagc
     3121 ggggcagtag taaggccttg cagaaacgcc cggccccgga acgggccaaa ccgcgaaagc
     3181 acgagcccgg ccagcgtccc caggatgacg gctcccgtgg ccgtggaggc cgctacctgg
     3241 atagatagcc atgcggccga caggatctgg tggtccttga acagctgcac gtaccacttc
     3301 accgtgggcg aatgggcggc gtcccagacg gtcaccagac gggagttgtt gaacgagaaa
     3361 aagaccatcg acaggatcgg gatgtagagg aaccccagtc ccacccaaag catcgtcttg
     3421 ctgaacaggc cctgggttct catttcttct gggcctgcag ttctttgttc tggaaccgct
     3481 ggaaaatcat gatgggcacg accagcagca gaagcatggc gatggccacg gcgctggctc
     3541 tcggccagtc gcggttcagg aagaactcat cggacaagag cttggccacc atgagctggt
     3601 cggtacggcc gagcagcgaa ggaattacgt attcgcccac ggccgggatg aacaccagca
     3661 gcgagccggc gacgatgccg gggaacgaca gcggcaaggt gacgcgcagg aacgaacgga
     3721 tcggccccgc gcccaggtcc tgtgcggcct cgagcagcgt gttgtcgtgc ttctcgaggt
     3781 tcgaatagag cggcaggatc atgaacggca gataggagta aacgatgccg atgtagaccg
     3841 cgaagctggt gttcatcatg gccagcggtt cgtccacgag gcccaaccac ttgaacagca
     3901 cctggttgat gagcccatcg gtcttgagca ggccgaccca ggcgtagacc cgcagcagga
     3961 aggacgtcca gaacggcagg atgatgagca gcaggaagat gttgcgctgg gtcgggggcg
     4021 aacgcgatat ggcgtaggcc atgggataac cgagcgccag gcagaacacc gtggagaaaa
     4081 acgcgacctt gatcgagtag atccaggcgc tgacgtacag cggctcattg aaaagataga
     4141 cgtagctgcc gatcagaagt ttgatctgca gcgcgccctc ttccagccag ctgaacacgg
     4201 gttcgtacgg cggccggccg agcggcgaga actccgcgaa ggaaatcttg aggacgatga
     4261 gaaacgggat caggaagaag acgatcagcc agagataggg aatggcgatg accagccgct
     4321 gccccgtcca gttctgcacc acgtattccg accagcgtgg tggcccgtac cgcaacagga
     4381 acgtccacag gggtcccagg cgaccgtaag agtcgcgaga acccagggtg cgggcagctg
     4441 gtttcacggc cttgagcgga agggacgctt cattgcgtca ccacgaccgg gctggatcca
     4501 tgccctgtga gccaaacagt ctcgtcccag agaatgcgtt cgtcgtccga catgcgttcg
     4561 acgttcggca gcgtgacgcg cacgatcttg ccggtgtcta tcttcacgag gtagatagaa
     4621 acgtcgccca tgtaggcgat ttccttgacg acgcccttga cgcaattttc atgcgtttca
     4681 gcaggctggg cgcgcaggat gttgatcttt tccggccgga tggcggcgta gacgatggcg
     4741 cccggcgccg agctgatgcc gtgatcgacg tagatgatgc cgcccagttc gtccgacttg
     4801 atgcgcacgc ggtccggcag gtcttcgacc atggtgcctt cgaacatgtt caccgagccg
     4861 atgaagtcgg cgacgaactt gttctgcggg aactcgtaga tttcggtggg cgtgccgatc
     4921 tgcacgattt caccgcggtt catgacgccg atgcgcgagg ccagcgtcat ggattcttcc
     4981 tggtcgtgcg tgacgacgat gaaggtgacg ccgagctgct cctggatgtt gatgagctcg
     5041 aactgcgtcg cttcgcgcag cttcttgtcg agcgcaccga gcggctcatc gagcagcagc
     5101 agcttgggac gcttgaccag cgcacgcgcc agcgcgacgc gctgacgctg gccgcccgag
     5161 agctggtgcg ggcgtcgcgc cgcgaactgg tcgagcttca cgatcgacag gatgtcggtg
     5221 actcgcgtgg cgatgtcggc gcgcgacagc cccttctcct gctcgaggcc gaacgcgatg
     5281 ttcttctcca ccgtcatgtg gggaaacagc gcgtacgact ggaacatcat gttgaccggc
     5341 cgctcgtacg gtggaacacc ggccatgtcc tggccgtcga gaaagatgga gccggagctc
     5401 ggattctcga agcccgccat catccgcagc agcgtcgact tgccgcagcc cgagccgccc
     5461 agcaggcaga agatttcgcc cttgaagatc ttcagcgtga cgttgttgac ggcgacgaag
     5521 tcaccaaacc gtttggtgac attctcgatg cgcacgtaag cctcggcgtg cggatcacgc
     5581 caaggttcgt tttttatttt cttgtctgta cgtcgttccg ccgcttgcat ggcaggcccc
     5641 cgttgcaatg aagagaacga tcgccgctcc ctaagcggcg accgtcagga tcatttgccg
     5701 gtacggaagc gcgtccaggt gcgcgtcagt gttcgggtga actcggcact ctcaggcaga
     5761 tctggaacca gcttgggcat catttccggg gtcgggaaga ttcccggatt gttcttcact
     5821 tccgcggtga ccagcggcca cgaggcggcg ttgctgttgg cgaagctgat gaagttgctg
     5881 ttcttggcgg ccacttcggg gcgcagcatg tagttgatga acacgtgtgc gttcttcaca
     5941 tgcgaagcgt cggcgggaat ggccatgctg tcgaagaaca tgaccgcgcc ttccttcgga
     6001 atgttgtact tgatggtgaa cggcttctgc gcttccgcgg cacggtcgcg agcctgcagc
     6061 gtgtcacccg accagcccag cgcgaggcag atttcgccat tggcgagatc ttcgatgtac
     6121 ttggacgagt tgatgaggcg gatgtacggg cgggccgaga gcagcacttt ttcggcggcc
     6181 ttcaggtctt ccgggctctc ggaattcgcg tccttgccca ggtagatgag cacggtgccg
     6241 atgatttccg acggcgcgtc gagcacggaa acgccgcact tggcgaactt ggaaaccacc
     6301 ttcgggtccc agaacatcgc gaaactatct accggcgcat ccggcatcgc ggccttgatg
     6361 gcctcttcgt tgtaggccac gcccgaggtg ccccacatgt agttcacggc gtgttcgttg
     6421 cccgggtcga agacttcgag ccgcttggtg atgtccggat cgaggttctt gagattgtcg
     6481 agcttggact tgtcgagctt ctggtagatg cccgacttga tctggcgggc gaggaagttt
     6541 gccgagggga ccaccacgtc gtatccggtg cggccggcga cgagcttggt ttccaggagc
     6601 tcgttggagt ccatgacctc gtagttgacc ttgatgccgg tttctttggt gaattcgtcg
     6661 agcaccgaag gctcgatgta gtcggaccag ttgtagacgt tgagctcttt ctcagcgtcg
     6721 gtgtcgtagg caggtgtcgc gggagcggcg gcggccggtg gcggcgcttc ttcttttttg
     6781 ccgcaggcga caagcaggaa aatacaggaa gccgaaacca gagccgaaag gctgcgagtg
     6841 gacattttta gacccctaaa tgtggtcgcg ccgtgagacc cacacggcgc gtctttagac
     6901 acgttatata acagcgaccg cgtctatagc cagccctcgg ctcgcgcttg agtagcggtt
     6961 tcatcgagcg atcgcactac tttggacagt agttcgtcga tttcgctccg agatatgacc
     7021 aaaggcggcg cgatgaacag cgtgtcgcgc gtcgcgcgca tgacgagacc gttgcgcgtg
     7081 cagttgtctc gcattcgcag gcccacctcg ccgcgcggct cgaagaaacg ccgcgaatgc
     7141 ttctcgggca ccagttcgat ggcggccagc aggcctgtgc agcgagcctc tcccaccagg
     7201 ggatgagacg cgagttcgct ccagcgggcg gctagatagg gtgcggcttc ttcccggacc
     7261 cgttccacga tgcgttcgcg gcggaatatt tcgaggttgg cgatggcgac cgcgcaggcc
     7321 gccgggtgcc cggaataggt gaaaccatgg aaaaactccc cgcccttgtc gataaggacg
     7381 ttggcgaccc ggtccccgac catgaggccg ccgatgggta gatagcccga ggacagtgcc
     7441 ttggccatcg tcatgaaatc cggacgcgtt ccataatgct cgcaaccgaa ccactggcct
     7501 gtacgaccaa agccgcagat gacctcgtcg gacgcgagca ggatcccgta cttgtcgacg
     7561 atccgctgga tctcgggcca gtaggtatcg ggcggaatga ccagcccacc ggcgccctgc
     7621 acgggctcgc cgatgaacgc agcaatgcgc tcgggtccca cctcgagaat tttcgattcg
     7681 agttcgcgag cgcatttcag gccgaactcc gccggcgaga gatctccacc ttccccaaac
     7741 cagtaaggtt ggttgatgtg cacgatgcct ggcaatgcgc catttggccc aagctgctcg
     7801 tgcatgggct tcatgccacc gagagatgcg ccggcgacgg tgctgccgtg atacccgttc
     7861 cagcgtgaaa cgatcacgtt ccgctcgggc tgatcgagca actgccaata gcggcggacc
     7921 agccggatca gcgtgtcgtt ggcttcgctg cccgagcccg tatagaaaac gtggttgaac
     7981 tgtgggggtg tcacctcttt gaggaggcgc gcgagctcga tggccggtgt cgtcgtgctc
     8041 tggaagaaac tgttgtagta aggcagctcg agcaattgtt tgtaggctgc gtctgacaat
     8101 tccttgcgcc cgtaccccag cgccacgcac cagagccccg acatgccatc gaggatcttg
     8161 tcgccctccg acgtgtagat atagacgcct tcggcgcgcg tgacgatgcg cgctcccttg
     8221 gccgccagct gcttgtggtc ggtgaagggg tgcaggtagt gagcggagtc cagtcgctgc
     8281 cagccactcg tgtcggtcgg gttagcactc acagcgttca ctccagcgtc agacgttcaa
     8341 cagaaggtgt tcccgttccc acgaactgat cacctgcagg aagacttcgt attcggcctc
     8401 cttgacgatg gtgtacgcgg agatgaacgg gtcgcccagg atgcgcacca gcggctcgca
     8461 ttcacgcagc tcgcggatcg cgtcgtcgag cgaacgcggc agcgagaacg gcagctcgtg
     8521 cgcactgccg gagatcggct ccgaaggctg cagaccctcg accatgccga gatagccgca
     8581 ggccagcgac gtggcgatgg cgatgtacgg attcgcgtcg gcgccggcga gacggttttc
     8641 cacgcggcgc gcctcggggt ccgacgccgg cacgcgcagg cccgcggtgc ggttgtcgta
     8701 gccccactgc acgttgatgg gcgcgagctg gaagcgcgag atgcgccggt atgaattgac
     8761 gttcggcgcc agcagcgcca tggccgccgg caggtatttc tgcatgccgg cgatgtgcga
     8821 gaaaaacagc ggcgtcggcg tgccgtccga attggagaaa atgttcttgc gagtcttggt
     8881 gtccaccacg ctctggtgga tgtgcatggc gctgcccggc tccttggcgt ggggcttggc
     8941 catgaacgtc gcgtacatct tgtggcgcaa cgctgcttcg cgcgccgtgc gcttgaacag
     9001 gaacgcctgg tcggcgagat cgagcgcgtt tccatgcagc aggttgattt ccatctgcgc
     9061 ggcgccgtct tcgtggatca gcgtgtcgat ctcgatgtcc tggtcttcgc agaactggta
     9121 catgtcgtcg aacagcggat cgaactcgtt gaccgcggcg atgctgtaac tctgccggcc
     9181 gggctcggcg cggcccgagc gaccgacggg cggcttgagt ggatagtcgg cgtcgatgtt
     9241 cggctcgacc aggtagaact cgagctccgg cgcgaccacc ggctcccagc cgcggttggc
     9301 gtacatctcg aggatgtggc gcagaacgcc gcgcggcgcc atgccgacgc gccgaccgtc
     9361 cgaatagaaa cagtcgtgga tgacctgcgc ggtcggttca gccgcccacg gaacgcgccg
     9421 caccgtcttg ggatcggcct tgaggatgat gtcgatctcg gccgggctca tcgccttgtc
     9481 ggtgtccggc gggtagtcgc cggtcacggt ctgcaggaag atgctttccg ggaggcgcat
     9541 gccttcgtct tcggcaaact tttccgccgg catgatcttg ccgcgcgcga tgcccgccat
     9601 gtcggggatg atggcctcga cttcggagat gccgtgatcg cggaggaatg ttctgatttc
     9661 gtttgccatt ggaactacct aactactttt gccttggtac gcgccgcctt gccgaactcg
     9721 gcaaacagcg ccttcgaaaa cggattgctc atcacctgcc actccgggtg ccactgcacc
     9781 gcgagcgcga acgccggcgc cgatgccacg cgaaacgcct cgaccagccc atcggtggcg
     9841 cgcgcctcga cggcgaggcc ggggcccagc ttgtccacgc cctgccaatg cagcgaattc
     9901 acgcggatgc gctcctgccc cgcgatcccg cgcagtaccg atcccggttc cagcacgacg
     9961 tcgtgcgccg gcccgtactg cacttcgagc ggctgctcct tgtcctcgcg atggtcatcg
    10021 aggcccatca cttcgtgcac cctctggtgc agagtgccgc ccatcgccac gttcatctcc
    10081 tggaatccgc ggcacacacc gaacaccgga acgctctgct cgatcgcgcg cgggatcagc
    10141 gacaacgtgg tctcgtcgcg atgcggatcg tgcagcgtcc ccggcacgct cgcaggtccg
    10201 gcgtatctca ccggctcgac gttcgacggg ctgcccgaga aaaaaatgcc atcgacacgg
    10261 ctcaggagtt cggtcacgtc tagcggcgac tcgagcacgg gaatcagcag cggcaaacaa
    10321 tccgcggcct gaacgatggc ggcgatgtac ttctcgccga ccgcatgaaa cggatgtgga
    10381 ccgatcatgc gtcgatcggc gggaatgcca atgacgggtt ttttctcgag tgagctagcc
    10441 atacgctttc acgcgggcct tcaggcccgg gcgctgtcta ttatctttta cggattcgca
    10501 cctgcgacca gggcgagggc cgtcattaga ccacagcgga gggtcatccg ggtcggacca
    10561 ttgctcggct catgcgggcc ttcgaagggc ggaaattgac tgtttggccc gtttgccggc
    10621 gcacaggcgg ccggcatatg ctgctgcgcc ccggcgccgc tcggcgtcga aaataataga
    10681 cactgagaag attatgacgc gcgcccctga acccgacct
//
LOCUS       DATDEU010000495         6289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_676972, whole genome shotgun sequence.
ACCESSION   DATDEU010000495 DATDEU010000000
VERSION     DATDEU010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676972"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /gene="rplW"
                     /locus_tag="VIV63_11850"
     CDS             complement(<1..324)
                     /gene="rplW"
                     /locus_tag="VIV63_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY6125335.1"
                     /translation="MSAEKSAKAVKAASAEKAPKVAKAPKVKKAKVVDIKDAPKKVAS
                     QEKLINILIAPHITEKTSLAMQDNNSYAFRVLRNSTKPEIKAAVELMFGVKVANVNVV
                     NETGKT"
     gene            complement(321..926)
                     /gene="rplD"
                     /locus_tag="VIV63_11855"
     CDS             complement(321..926)
                     /gene="rplD"
                     /locus_tag="VIV63_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY6125336.1"
                     /translation="MELKVINGGATLKVSDETFGKEFNQSLVHQVVTAYRNGGRAGTK
                     AQLTKAEVRGGGRKPRPQKGGGTSRAGSTRSPIWVGGGRAFAAKPRDFAQKVNKKMYR
                     GAIQSMLSELVRQDRLVVTQAFTVDAPKTKLVAAELKKLGLEKVLIIVEGIDEKLFLG
                     ARNLPHVEVLPVALLNPLSLVSYDKVLITVGAVKLIEERLQ"
     gene            complement(939..1643)
                     /gene="rplC"
                     /locus_tag="VIV63_11860"
     CDS             complement(939..1643)
                     /gene="rplC"
                     /locus_tag="VIV63_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY6125337.1"
                     /translation="MSVALIGRKAGMTRIFTDAGETVPVTVIEVLPNRITQVKSVDKD
                     GYRAIQVSFGKKRPQLLSKAAAGHYAKANVEPGKAMVEFRLKEKEGTDLAVGSELKAG
                     IFEAGAIVDVTGTTIGKGFAGTMKRHNFAGHHASHGVSVSHRTPGSIGQRQTPGRVFQ
                     GKRMSGHMGVVRRTIENLKVIEIDVERNLLLIRGAVPGAEGGQVIVRPSVKAAAQKLR
                     KKIAPAKINTGTSNKK"
     gene            complement(2022..3743)
                     /locus_tag="VIV63_11865"
     CDS             complement(2022..3743)
                     /locus_tag="VIV63_11865"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HEY6125338.1"
                     /translation="MPGQLTISLGQYSDKGRKESNQDFHGALIPGEPQLSEKGIAIAV
                     ADGISSSNVSQVASQSAVRVFLEDYYCTSPAWSVKKSAHTVLMAVNSWLFAQTQQSPY
                     RYEKDRGYVCTISALVFKSATAHLFHAGDTRIYRLRDGVLEQLTNDHRVWVSNDQSYL
                     SRALGIHSHLDIDYQSMRLEQGDVFVFATDGVHEFANTRTIINAVKDHSGDLDAAARL
                     IAGEALERGSKDNLTAVIVRVDAVPEQSAKEVHRQLTELPFPPDLVPRMVMDGFTIVR
                     ELHASSRSHVHLATFDPAEAPVVIKTLSTEMRADVAHLDRFLMEDWVAQRISNPHVVK
                     AAARGRRRNFLYTVTEYVDGQTLHQWLIDHPKPPVETVRGIIGQIANGLLAFHRLEML
                     HQDLRPQNVMIDAAGTVKLIDFGSTRVAGISEIDSPLERGAMLGTLQYTAPEYFLGES
                     GSTRSDLFSLAVIAYQMLSGRLPFGADVSRARTRAAQRQLKYQTVLDDEREIPAWIDD
                     VLRKATHPDPNRRYEELSEFVYDLRHPTADYLSRTRAPLLERNPVAFWRGVSLILAIV
                     VIVLLAS"
     gene            complement(3810..4631)
                     /locus_tag="VIV63_11870"
     CDS             complement(3810..4631)
                     /locus_tag="VIV63_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEY6125339.1"
                     /translation="MSYLAPAEFVTKMVDAGESKVFMSTRDTLIRAFMAGAILALAAV
                     FAVMVTQQTGYPIIGAILFPVGFCMLYLLGFDLVTGVFVLAPLALLDKRPGVTFGGVM
                     RNWGLVFCGNFAGAFTVAVLMSIVFTFGFTTDPNPIGQFIAHVGESRTIGYKEHGMGG
                     MVTLFVRAMLCNWMVSTGVVGAMISTSVGGKVLAMWMPIMVFFAMTFEHSVVNMFLFP
                     SGILMGANFNWADYFLWNEIPTILGNIVGGLAFTGLTLYSTHIRTAPKRNLKLAA"
     gene            complement(4674..5954)
                     /locus_tag="VIV63_11875"
     CDS             complement(4674..5954)
                     /locus_tag="VIV63_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125340.1"
                     /translation="MTSRTHARCAATAGLLGCLLAASAAQADGLDPVWGAFGETKPIL
                     DTRLRSETVHQDGIPETGHAFTLRARLGLETGKAWNTSLLIEGDANMPITDKYRPDPA
                     VPTELMYPVVADPEAYELNRFQITNTSLPGTTLTLGRQRIGLDDQRFVGAVGWRQNEQ
                     TFDALRAVNRSVANLVLDVTYLNRANRVFGDDSPQGDYKGDSFLLNAGYQTKIGKLTA
                     FGYLLDFEAITGIPAGMNPIRDSTSTYGLRFAGEKPAGKIKLAYMASYATQTDYADNP
                     LDLDLDYLTGELTATFRQFSLGVGTEILEGNGLPGLAGKGFTTPLATLHKFQGWADKF
                     LTTPANGIEDLYATGGVTLKGVGSLDTLGLVVSYHDYNAEQITADYGHEWNVSLAAKY
                     KRVNLMLKFADYEQGVPTIARSTEKVWGQIEFVW"
     gene            complement(5992..>6289)
                     /locus_tag="VIV63_11880"
     CDS             complement(5992..>6289)
                     /locus_tag="VIV63_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125341.1"
                     /translation="LDALTRWDLQEVLMDILRRTKITAICVTHDVDEAILVADRVIMM
                     TNGPRARLGKVMLVDLPRPRTRKMLLEHPRYYAYREELLSFLEEYEHGAGNRAA"
BASE COUNT         1106 a         1941 c         2006 g         1236 t
ORIGIN      
        1 ggtcttgccg gtctcgttca ccacgttcac gttggcgacc ttgacgccga acatgagctc
       61 gaccgcggcc ttgatttccg gcttggtcga attacgcagc acgcggaacg cgtacgagtt
      121 gttgtcctgc atcgccagcg aggtcttttc ggtgatgtgc ggcgcgatca ggatgttgat
      181 cagcttttcc tgactggcaa ccttcttcgg cgcgtccttg atgtcgacga ctttcgcctt
      241 cttcaccttg ggggccttcg cgaccttcgg cgccttttcg gcgctcgcgg ccttcacggc
      301 cttggcggac ttctcggcgc tcattgcagg cgctcctcga tgagtttcac agcgccgacg
      361 gtaatgagca ctttgtcgta gctcacgagc gacagcgggt tcagcagggc gaccggcagc
      421 acttcgacgt gcggcagatt gcgggcgccg aggaacagct tctcgtcgat gccctcgacg
      481 atgatcagca ccttctcgag gcctaacttc ttgagctcgg cggcgaccag cttggtcttc
      541 ggcgcgtcga ccgtgaaagc ctgcgtgacc accaggcgat cctggcgcac gagctccgac
      601 agcatcgact ggatcgcgcc gcggtacatt ttcttgttca ctttctgcgc gaaatcgcgc
      661 ggcttcgcgg cgaacgcacg gccaccgccg acccagatgg gcgaacgggt cgagccggcg
      721 cgcgaggtgc cgccaccttt ctgcggacgc ggcttgcggc caccgccgcg cacttcggcc
      781 ttggtcagct gggccttggt gcccgcgcga ccgccgttgc ggtaagcagt aacgacctgg
      841 tgaaccagcg actggttgaa ttccttgccg aaggtttcgt cggaaacctt gagcgtggcg
      901 ccaccattga tgactttgag ttccatgtgt gccgccaatt acttcttgtt ggacgtgccg
      961 gtgttgatct tggccggcgc gatcttcttg cgaagcttct gcgcggcggc cttcaccgac
     1021 ggacgcacga tgacctggcc accttcggca cccgggacag cgccgcggat gaggagcagg
     1081 ttacgttcga cgtcgatctc gatgaccttc aggttttcga tcgtgcggcg cacgacaccc
     1141 atgtggcccg acatgcgctt gccctggaac acgcgtcccg gggtctggcg ctggccgatg
     1201 gagcctggcg ttctgtgcga cacggacacg ccgtgcgacg catggtggcc ggcgaagttg
     1261 tgacgcttca tggtgccggc gaagcccttg ccgatcgtgg tgcccgtgac gtcgacaatg
     1321 gcgcccgctt cgaagattcc ggccttgagc tcggaaccga cggcgagatc agtgccttct
     1381 ttttccttca ggcggaattc caccatcgcc ttgccgggtt cgacattcgc tttcgcgtag
     1441 tgaccggcgg cggccttcga caggagctgc ggacgcttct tgccgaaaga aacctgaatg
     1501 gcgcgataac cgtccttgtc gacggatttc acctgagtga tgcggttcgg cagcacttca
     1561 atgacggtta ccggaaccgt ctcgcccgca tcggtaaaga tgcgagtcat gccggccttg
     1621 cggccaataa gagcaacgct catgactatc ctctcgttcc tgcatgcgct tcgattggcg
     1681 cttgcggtgt agctaggtct aacgagggaa cactggagct tggcggcggc cagaagggcg
     1741 cgccgcgatg ctcgagtggt cttgggtttg gagcttcgaa ggctcgccca acctcgcccg
     1801 atttcgcttg aaatcaatcg cttgagactt tggtcccagg cgaaagggcg cgcagtatat
     1861 gcagcgaccc cttggcccgc aagcccggtt tacctgcctc ggatcccggc cacacctata
     1921 taaggtgtcg ccgccgggcc ctgcaggcgc ccgcccgggc tcgagtccat taactcatta
     1981 aatatcaata gtttatgggc ctcggaccgc ccaggtttgc ttcagctggc cagcaacacg
     2041 atcaccacga tcgccagaat cagagatacg ccccgccaga aggccaccgg attgcgctcg
     2101 agcaggggcg cgcgcgtccg gctgagatag tccgcggtcg ggtggcgcag gtcgtacacg
     2161 aactccgaca gctcttcata tcgcctgttg ggatccgggt gagtcgcctt gcgcaggacg
     2221 tcgtcgatcc acgccggaat ctcgcgttcg tcgtcgagca cggtttgata cttcaactgg
     2281 cgctgcgcgg cgcgcgtacg cgccctggag acgtcggcgc cgaatggcag tcggcctgac
     2341 aacatctggt aggcaatgac cgcgagcgaa aacagatccg aacgcgtgct gccgctctcg
     2401 cccaggaaat attcgggcgc ggtgtattgc agcgtgccca gcatggcgcc gcgctcgagc
     2461 ggcgaatcga tctcggagat gcccgccaca cgcgtcgagc cgaagtcgat gagcttcacg
     2521 gtgccggccg cgtcgatcat cacgttctgc gggcgcaggt cctggtgcag catctccagg
     2581 cgatggaacg ccagcaggcc attggcgatc tgcccgatga ttccgcgcac cgtttcgacc
     2641 ggcggcttcg gatgatcgat gagccattga tgcagcgtct ggccgtcgac gtattcggtg
     2701 acggtgtaca ggaaattgcg gcggcggccc cgcgccgcgg ccttcaccac gtgcggattg
     2761 ctgatgcgct gcgccaccca gtcttccatg aggaaacggt cgaggtgcgc gacgtccgcg
     2821 cgcatctcgg tagatagggt cttgatcacc accggcgctt ccgccggatc gaaggtggcc
     2881 aggtgcacgt ggctgcggct gctcgcgtgg agctcgcgca cgatcgtgaa tccgtccatg
     2941 accatgcgcg gcacgagatc cggcgggaac ggcaactcgg tgagctgccg gtgaacttct
     3001 ttcgcgctct gctcgggcac ggcgtcgacg cgcacgatga cggccgtgag gttgtccttg
     3061 ctgccgcgct cgagcgcttc gccggcgatc agccgcgccg cggcgtcgag atcgccgctg
     3121 tgatccttca ccgcattgat gatcgtgcgc gtgttcgcga actcgtgcac gccatcggtg
     3181 gcgaacacga agacgtcgcc ctgctccagc cgcatggact ggtagtcgat gtcgagatgc
     3241 gagtggatgc cgagcgcgcg gctgagataa ctctgatcgt tcgagaccca cacgcggtgg
     3301 tcgttggtca gctgttcgag gacgccgtca cgcagccggt agatgcgcgt gtcgccggcg
     3361 tgaaacaggt gcgcagtggc ggacttgaac acgagcgcgc tgatcgtgca cacgtaaccg
     3421 cggtctttct cgtagcgata cgggctctgc tgggtctgtg cgaacagcca ggagttcacg
     3481 gccatgagca cggtgtgcgc ggacttcttc accgaccacg ccggtgacgt gcagtagtaa
     3541 tcctcgagga atacgcgcac cgcggactgg ctggctacct ggctgacgtt gctgctgctg
     3601 atgccatcgg cgacggcgat ggcgatgccc ttctccgaga gctgcggctc accgggaatg
     3661 agcgcgccat ggaaatcctg gttggattcc ttgcgcccct tgtcggaata ctggcctaac
     3721 gagattgtga gttggccggg catgaatcac gcagagtgta taggtaaccc cgggccgcgc
     3781 gaggcccggg gttccttttc aacctgcctt caggcggcga gcttcaggtt gcgcttcggc
     3841 gcggtcctga tgtgcgtcga atacaacgtg aggcccgtga acgcgaggcc accgacgatg
     3901 ttgcccagga tggtcgggat ctcgttccag aggaagtagt cagcccagtt gaagttggcg
     3961 cccatgagga tgcccgaagg gaacaggaac atgttcacca ccgagtgttc gaaggtcatc
     4021 gcgaagaaca ccatgatcgg catccacatc gcgagcactt tgccacccac cgaagtggag
     4081 atcatcgcgc caacgactcc agtggaaacc atccagttgc agagcatggc gcgcacgaac
     4141 agggtgacca tgccgcccat gccgtgttcc ttgtagccga tcgtgcgcga ctcgccgaca
     4201 tgcgcgatga actgcccgat cggattcgga tccgtggtga atccgaacgt gaagacgatg
     4261 gacatgagca ccgcgacggt gaaggcgccg gcgaaattgc cgcagaacac cagaccccag
     4321 ttgcgcatga cgccaccgaa ggtcacgccc ggacgcttgt cgagcagcgc cagcggcgcc
     4381 agcacgaaca cgcccgtgac gagatcgaac ccgagcaggt agagcatgca gaagcccacc
     4441 gggaacagaa tggcgccgat gatcgggtag cccgtctgct gcgtcaccat gaccgcgaac
     4501 acggccgcga gcgcgagaat cgctccggcc atgaatgcgc ggatgagcgt gtcgcgcgtc
     4561 gacatgaaaa ctttcgattc gccggcgtcg accatcttgg tgacgaactc tgctggagcc
     4621 aggtaagaca tcgatctcct cccctatcta cttaaagaac actgacagtg tgattaccaa
     4681 acgaactcga tctgtcccca gaccttctcg gtgctgcggg caatggtggg cacgccctgc
     4741 tcgtaatccg cgaacttgag catgaggttc acgcgcttgt acttggcggc cagcgacacg
     4801 ttccattcat ggccgtagtc ggcggtgatc tgctcggcgt tgtagtcgtg atagctgacc
     4861 acgagcccca gcgtgtcgag gctgccgacg cccttgagcg tcacgccgcc cgtggcgtac
     4921 aggtcttcga tgccgttggc gggcgtggtc aggaacttgt cggcccagcc ctgaaacttg
     4981 tgcagcgtcg cgagcggtgt cgtgaagccc ttgccggcca gacccggcag tccattgccc
     5041 tcgaggatct cggtgccaac gcctaacgag aactggcgga acgtggcggt caactcgccg
     5101 gtcaggtagt cgagatcgag gtcgagcggg ttgtccgcgt agtcggtctg ggtcgcgtac
     5161 gaagccatgt aagccagctt gatcttgccc gccggcttct ctccggcgaa tcgcaatccg
     5221 tacgtcgacg tggaatcacg gatcgggttc atgcccgcgg ggatgccggt gatggcttcg
     5281 aagtcgagca gatagccgaa cgcggtcagc ttgccgatct tcgtctggta acccgcattc
     5341 agcaggaaac tgtcgccctt gtaatcgccc tgcggtgaat cgtcaccaaa gacgcggttg
     5401 gcgcggttga gataggtgac gtcgagcacc aggttcgcga cgctgcggtt caccgcgcgc
     5461 aacgcgtcga aggtctgttc gttctggcgc cagccgacag cgccgacgaa gcgctggtcg
     5521 tcgaggccga tacgctggcg accgagcgtc agcgtcgtgc ccggaagact ggtgttggtg
     5581 atctgaaatc ggttgagctc gtaagcctcg gggtccgcga ccaccgggta catcagctcc
     5641 gtcggcacgg ccgggtcggg ccggtatttg tccgtgatcg gcatgttcgc gtcgccttct
     5701 atcagcagcg acgtattcca cgccttgccg gtctcgaggc cgagcctggc gcgcagcgtg
     5761 aacgcatgtc cggtctccgg gatgccatcc tggtgaaccg tttccgagcg cagtcgcgta
     5821 tcgagaatgg gcttggtttc gccgaaagcg ccccagaccg gatcgagacc gtcggcctgt
     5881 gcggcactgg ccgcgagcaa acatccgaga agacctgcag tggcggcgca acgcgcgtgt
     5941 gtccttgacg tcatcgaaaa actcccaatt cgagaaaaca ggattttgtt gtcaggcggc
     6001 acggtttcca gcgccgtgct cgtactcttc cagaaagctc aacagctctt cccggtaggc
     6061 gtagtaacgc ggatgctcca gcagcatctt tcgcgtgcgc ggccgcggca ggtcgaccag
     6121 catgaccttg ccgaggcggg cgcgcggccc gttggtcatc atgatcacgc ggtcggcgac
     6181 caggatcgct tcgtccacgt cgtgcgtgac gcagatggcg gtgatcttcg ttcgcctgag
     6241 gatgtccatg aggacctcct gcagatccca gcgcgtcagc gcgtccagc
//
LOCUS       DATDEU010000496         2965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1552597, whole genome shotgun sequence.
ACCESSION   DATDEU010000496 DATDEU010000000
VERSION     DATDEU010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552597"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VIV63_11885"
     CDS             <1..380
                     /locus_tag="VIV63_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125342.1"
                     /translation="QLEYSQRLRDRARNVIGSADARSTGRVLFDGAVAGGARALGIEA
                     RDGVVGIVERAPADFLSLSEQSTALACREGDALLDTLIFAGGDGCIDGVWCAGKKLVT
                     QGRHHERDAVASRYRSALQRLML"
     gene            500..1048
                     /locus_tag="VIV63_11890"
     CDS             500..1048
                     /locus_tag="VIV63_11890"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY6125343.1"
                     /translation="MVVLRFLRVLFFAVLVVSPGYSAAAPNEYQVKAVFLFNFSRFIE
                     WPDSAFPDAKAPFIVGVFGHDPFGPDLDAVVKGETVNGRALVVRRVRNAAEAAGCQIL
                     FIHQSENRRLDEVVAALASRHTLTVTDLPGTAQRGVMIRLVTEKGRVRMRINVESARA
                     ADLTINSNLLRSAEIVPAGGGT"
     gene            1051..>2965
                     /locus_tag="VIV63_11895"
     CDS             1051..>2965
                     /locus_tag="VIV63_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HEY6125344.1"
                     /translation="MPSTPVRRKLMRMVLLTSGIVILFTCAMFVTYEVVTFKQTIARQ
                     LEILGKAIAQNSTAALAFENPDDARTVLSAFRVDPHIVSAALYNRSGDLFAAYPEGVA
                     RKSLPASVQSAGYRFEDGHVIGFEPVRLDDKTLGTLFVKSDLNAIDERFGLYALIAAA
                     VTALAVLLAYFVANRQQSEISGPILDLAKTARAISEHHDYSVRAQSSGAQEIGELTDA
                     FNHMLTQIQQAEIRSRKQMGRLSLLQHITAAIGDRHDLPSIFQVVLRNVEENLPIDFG
                     CVCLYDSATTTLTVSTVGAGSRHLSVTLGLSNGSVVPIDENGLSTCIRGRLVYEPDTS
                     RLDFAFPQRFAVAGLQSLVIAPLLVEASVFGVFVASRRPANAFSSGDCEFLMQLSGHV
                     ALASHQARLHGALRQAYDELRQSQHTVMQQERLRALGQMASGIAHDINNAISPISLYT
                     DSLLEREPGLSDKARNSLIVIQRAIDGVAATVARMREFYRQREPEMTLALVDLNAVAN
                     QVIELTRAKWFDLPQQRGVSIELHMDLAGKLPGIMGAEGEIRDALTNLIFNAVDAMPE
                     GGILRVRTFARPGPGQSQLVRIEVTDTGIGMSEDTRRRCLEPFFTTKGERGTGLGLAM
                     VYGMIQRHSAEFDI"
BASE COUNT          513 a          961 c          958 g          533 t
ORIGIN      
        1 ggcaactcga atacagccag cgtctgcgcg accgcgcgcg caacgtgatc ggcagcgccg
       61 atgcgcgatc caccggccgc gtgttgttcg acggcgctgt cgccggcggc gcgagagcgc
      121 tgggtatcga agcacgcgac ggcgtcgtgg gaatcgtcga acgtgcgccg gcggatttcc
      181 tctcgctgtc cgaacaatcc acggctctcg cgtgccggga aggtgatgcg ctgctcgaca
      241 cgctgatctt cgccggcggc gatggctgca tcgatggcgt ctggtgcgcc ggcaagaaac
      301 tggtgacgca gggccggcac catgaacggg acgcagtggc ctcgcgttac cggagcgcac
      361 ttcagcgact tatgttgtag gaaagcctaa ctacaatcgt ctgcggttct gcgagtaacc
      421 ctgaggcgcc actttctccg catccggcgt gcaagaatgc gccgtgattc accgggttgg
      481 gggtcgcagg tcggtcgcga tggtagttct gagattcctg cgagtcctgt tctttgccgt
      541 gctggtggtg tcgcccggtt acagcgcggc tgcgcccaac gaataccagg tcaaagcggt
      601 attcctcttc aacttctcgc gattcatcga atggcccgac tcggcctttc cggacgccaa
      661 ggctccgttc atcgtggggg tcttcgggca cgatccgttc ggtcccgatc tcgatgcggt
      721 ggtgaaaggc gaaaccgtga acggccgtgc gctcgtggtg cggcgcgtgc gcaatgcagc
      781 agaagcggcg ggctgccaga tactcttcat tcatcagtcg gagaatcgcc ggctcgatga
      841 ggtcgtcgcg gcactcgcga gccgtcacac attgaccgtc accgatcttc cgggcacggc
      901 tcagcgcggc gtgatgatcc ggctcgtgac cgagaagggg cgagtacgca tgcgcatcaa
      961 cgtcgagtcg gcgcgcgccg cggacctgac catcaactcg aatctcctgc gatccgccga
     1021 gatcgtcccg gcgggcggag ggacctgagc gtgccgtcga ccccggttcg ccgcaagctc
     1081 atgcgcatgg tgctgctcac gagcggcatc gtgatcctgt tcacctgcgc gatgttcgtg
     1141 acctacgaag tcgtcacatt caaacaaact atcgcgcggc aactcgagat cctcggcaag
     1201 gcgatcgcgc agaacagtac cgcggcgctg gcgttcgaga atccagacga tgcgcgtacg
     1261 gtcctgtcgg ccttccgcgt ggatccgcac atcgtttcgg cggccttgta caaccgctcg
     1321 ggcgatttgt tcgcggcgta tcccgaggga gtcgcccgga agtcattgcc cgcatcggtg
     1381 caatcggcgg ggtatcgatt cgaagacggc cacgtgatcg ggttcgagcc ggtgcgcctc
     1441 gatgacaaga cgctgggcac gttgttcgtg aaatcggacc tcaacgccat cgacgaacga
     1501 ttcggtctct acgcgctcat cgcggccgcg gtcaccgcgc tcgcggtgct gctcgcgtac
     1561 ttcgtggcca accggcagca gtcggaaatt tccggcccca ttctcgacct cgcgaaaacc
     1621 gcgcgcgcga tttccgaaca ccacgactac agcgtgcgcg cgcagtcgtc gggcgcccag
     1681 gagatcggcg agctcaccga cgcgttcaac cacatgttga cgcaaatcca gcaggccgaa
     1741 atccgttcac gcaagcagat ggggcggctc tcgctgctgc agcacatcac ggccgcgatc
     1801 ggcgaccgcc acgacctgcc cagcatcttc caggtggtgc tgcgcaacgt cgaagagaac
     1861 ctgcccatcg atttcggctg cgtgtgcctg tacgactccg cgaccaccac gttgacggtg
     1921 agcacggtcg gcgctggcag ccggcacttg tccgtcacgc tgggcctgag caatggctcg
     1981 gtggtcccca tcgacgagaa cggattgtcg acctgcatcc gcggccgcct ggtttacgaa
     2041 cccgacacgt cgcggctcga tttcgcgttt ccgcagcggt ttgcggtggc gggactgcag
     2101 tcgctggtca tcgcaccgct gctcgtcgaa gccagcgtgt tcggcgtgtt cgtggcgtcg
     2161 cgtcggcccg cgaacgcatt cagcagcggc gattgcgagt tcctcatgca gctctccggc
     2221 cacgtggcgc tggcgtcgca ccaggcgagg ctgcacggcg cgctccggca ggcttacgac
     2281 gaactgcgcc agtcgcagca cacggtgatg cagcaggagc ggttgcgggc gctcggccag
     2341 atggcgagcg gtatcgccca cgacatcaac aacgccattt caccgatctc gctgtacacc
     2401 gactcgctgc tggagcgcga gcccggcctg agcgacaagg cgcgcaattc cctcatcgtc
     2461 atccagcgcg ccatcgacgg cgtcgccgcc accgtggcgc gcatgcgcga gttctaccgg
     2521 caacgcgagc cggagatgac gctcgcgctg gtggatctga acgccgtggc caaccaggtg
     2581 atcgagctca cgcgcgccaa gtggttcgac ctgccgcagc aacgcggcgt ctccatcgag
     2641 ctgcacatgg atctggcggg caaactaccc ggcatcatgg gcgccgaggg cgagattcgc
     2701 gacgcgctga ccaatctcat cttcaatgcg gtcgatgcga tgcccgaggg tggcattctg
     2761 cgcgtgcgga ctttcgcgag acccggtccc ggccaatcgc aactggtcag gatcgaagtg
     2821 accgacaccg gcatcggcat gagcgaggac acccgtcgtc gctgcctaga gccgttcttc
     2881 acgaccaaag gtgagcgggg tacgggactg gggctcgcca tggtctacgg catgatccag
     2941 cggcacagcg ccgagttcga tatcg
//
LOCUS       DATDEU010000497         4307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1843900, whole genome shotgun sequence.
ACCESSION   DATDEU010000497 DATDEU010000000
VERSION     DATDEU010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843900"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VIV63_11900"
     CDS             complement(<1..1603)
                     /locus_tag="VIV63_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY6125345.1"
                     /translation="MATSNQFDAIVVGSGMSGGWAAKELTEKGLKTLVLERGRNIRHV
                     TDYTTALKDPWQLPHNNRPTNADRAAQPIQSTLYLYDQSTKQHFVDDLKHPYVQAQPF
                     NWFRGYQVGGRSLMWARHVFRYSDLDFEANLREGVGIDWPIRYADIKPWYEHVERFIG
                     ASGENSGLPQLPEQILQPPFEMNALEKHIRSHMAAAFNGERKLICSATAVLSREHNGR
                     GPCQGRNQCARGCPYSAYFSSNGVTLPAAAATGNLTLQPDAVVHSLIFDEGKNKVTGV
                     RVVDAITRKTTEYSARVVFLCASALNSTAILLNTRSSRFPEGLGNDSGELGHNLMDHH
                     NGGGATASFSGLADRYYRGRRSTSMYIPRFRNVSKREDKFRRGYGYEVYTSRGSWTRG
                     IGSEDFGAGFKNEITRPGEWNLYMEGYGETLPYHDNRMWLSAETDSWGMPFIHLSMSY
                     RDNERAMAQQMTDDAVEMMQAAKLENVKGFNNPVTPGSVIHEMGTARMGRDAKTSVLN
                     AHNQVHAVPNVFVTDGSCMVSSPTQN"
     gene            complement(1603..2124)
                     /locus_tag="VIV63_11905"
     CDS             complement(1603..2124)
                     /locus_tag="VIV63_11905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY6125346.1"
                     /translation="MSDRRSFLRGASVLMGHAAFGQVMTAFAAAPRKATFFTESEMQT
                     LRALVDVVLPATDSPAASAADTHFFIDMAIPACANAAAQKAFREGLKALGQKKFAQLV
                     PVDQVAMLKSRAAADMALDYDRSFFKILKDYTLTGYFLSEVGATQALAYEKVPGGFQG
                     DLPLAANQKAWAI"
     gene            complement(2161..2397)
                     /locus_tag="VIV63_11910"
     CDS             complement(2161..2397)
                     /locus_tag="VIV63_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125347.1"
                     /translation="MTSGAWLDAVQWPAMAVTLFAAWLVASRHKTRRAHGFWWFIASN
                     VLWVVWAWHVQAWALIALQAGLFAMNVRGALKNE"
     gene            complement(2404..2898)
                     /locus_tag="VIV63_11915"
     CDS             complement(2404..2898)
                     /locus_tag="VIV63_11915"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6125348.1"
                     /translation="MQFSQKISPCLWFDSQAEEAANFYVSVFKNSKILDVARYGKVGY
                     ETHQKPEGSVMTVIFEIEGVPFTALNGGPIFKFSEAVSLQIYVEDQKELDYYWNKLTA
                     GGDPKAQVCGWLKDRFGLSWQVVPSKMVEWFTRPDDKSQRAFAAMMKMGRLDIAALEK
                     AYHG"
     gene            complement(3011..>4307)
                     /locus_tag="VIV63_11920"
     CDS             complement(3011..>4307)
                     /locus_tag="VIV63_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY6125349.1"
                     /translation="GFDGSLFTGAVGFIESEFELNEFELGWAVTSMAVAATVAIFISG
                     PLADRIGRRTVLRLATCIFLISALLAALSANFATLIVARLLSGFGVGAVLVTAPMYIA
                     EISPSAWRGRMVSFNQLFIVLGQLAAFGSNLFIASLGGDADWSWRWMLGIGAIPAIPY
                     FFAMLMVPESPRWLAMHDRLPAARSVLARLTGSERADTELEAVLVSLEHEAQRAKPAL
                     SEVLSPALRGVIIIGLLVGVLQQITGINSVLSYAVVIFEKAGAGANAPFMQTVWVGFV
                     NVVFTVLALLLFDRVGRRPLLMFGCFGIGTFMLLAAYGFQVENEAQSSLIVLIGMLGF
                     VASFALSLGPGMWVLFSEIFPNRIRGLAISCVGVVNSTVCVIVQFVFPWEMKTIGSSN
                     TFLIYGVFALAGVAFIWRLVPETRGRSLEELEDALVRRS"
BASE COUNT          837 a         1350 c         1311 g          809 t
ORIGIN      
        1 ggttctgcgt gggcgacgac accatgcagc tgccatcggt gacgaataca ttcggcactg
       61 cgtgcacctg gttgtgtgcg ttgagcaccg atgtctttgc atcccggccc atgcgtgccg
      121 tgcccatttc gtggatcacg ctgccgggcg tcacggggtt gttgaagccc ttgacgttct
      181 cgagcttcgc cgcctgcatc atctccacgg cgtcgtcagt catctgctgc gccatggcgc
      241 gctcgttgtc gcgataactc atcgacaggt ggatgaaggg catgccccag ctatcggtct
      301 ccgccgagag ccacatgcgg ttgtcgtggt acggcagtgt ctcgccgtaa ccttccatgt
      361 acaggttcca ttcacccggc cgcgtgattt cattcttgaa gcccgcgccg aaatcttcgc
      421 tgccgatgcc acgtgtccag cttccgcgcg acgtgtagac ctcgtagcca tagccgcgcc
      481 tgaacttgtc ttcgcgcttg ctgacgttac ggaatcgcgg gatgtacatg ctcgtcgatc
      541 ggcgcccgcg gtaataacgg tccgcgagtc ccgaaaaact ggccgtggcg ccgccaccgt
      601 tgtggtgatc catgaggttg tgccccaact ctccgctgtc gttgcccagc ccttcgggaa
      661 aacgcgatga gcgggtattg agcaggatcg ccgtggaatt caacgccgac gcgcacagga
      721 acaccacgcg tgcggagtat tcagtggtct tgcgcgtgat ggcgtcgacg acgcgcacgc
      781 cagtcacctt gttcttccct tcgtcgaaga tcagcgaatg caccaccgcg tcgggttgca
      841 gcgtgagatt tcccgtcgcc gccgcggccg gcagcgtgac tccgttcgaa gagaaatagg
      901 cgctgtacgg acagccgcgc gcgcattggt tgcggccctg gcaaggtcca cggccgttgt
      961 gctcgcggga caacactgcc gtcgcgctgc agatgagctt gcgttcgccg ttgaaagccg
     1021 cggccatgtg actgcgaatg tgtttctcga gcgcgttcat ttcgaatggc ggctgcagga
     1081 tctgctcggg caactgcggc aggccggagt tctcgccact ggcgccaatg aatcgctcga
     1141 cgtgttcgta ccagggtttg atgtcggcgt agcgaatcgg ccagtcgatg ccgacgcctt
     1201 cgcgaaggtt ggcttcgaaa tccagatcgc tgtagcggaa cacgtggcgc gcccacatca
     1261 gcgagcggcc gccgacctgg tagccgcgaa accagttgaa cggctgagcc tgcacgtacg
     1321 gatgtttcag gtcgtcgacg aaatgctgtt tggtcgactg gtcgtataga tagagtgtcg
     1381 actggatggg ctgcgccgcg cggtcggcgt tggtgggccg gttgttgtgc ggcagttgcc
     1441 acgggtcctt gagtgccgtc gtgtagtcgg tgacgtggcg gatgttgcgt ccgcgctcga
     1501 gcacgagggt tttcaatcct ttttcggtga gctctttggc ggcccatcca cccgacattc
     1561 ccgacccgac gacgatcgcg tcaaattgat tggaggtagc catcagattg cccacgcttt
     1621 ctggttggcc gcgagcggca agtctccctg gaatccgccc ggcacttttt cataggccag
     1681 cgcctgcgtg gcgcccacct cggacaggaa gtacccggtg agcgtgtaat ccttgaggat
     1741 cttgaagaac gaccgatcgt agtcgagcgc catgtccgcg gcggcgcgcg acttgagcat
     1801 cgccacctgg tcgaccggca ccagttgcgc gaacttcttc tggccgaggg ctttcaggcc
     1861 ttcgcggaat gccttctggg ccgcggcatt ggcgcaggcg gggatggcca tgtctatgaa
     1921 gaaatgagtg tcggcggcgg aagcggccgg cgaatcggtg gccggcagga ccacatccac
     1981 cagcgcacgc agcgtctgca tttcggattc ggtgaaaaag gtggccttgc gaggcgcggc
     2041 ggcgaacgcc gtcatgacct ggccgaacgc ggcatgcccc atgagtacgg aagcgccgcg
     2101 tagaaacgaa cgacggtcag acatgatccc cctcccggat cgaatgaacg ccgtcggatc
     2161 ttattcgttc ttcagtgctc cgcgcacgtt catcgcgaac aatcccgcct gcaatgcgat
     2221 cagcgcccag gcctggacat gccaggccca gacgacccac aacacgttgc tcgcgatgaa
     2281 ccaccagaag ccgtgcgcgc gccgcgtctt gtgacgcgac gccaccagcc aggcggcgaa
     2341 cagcgtcaca gccatggcgg gccactggac cgcgtccagc caggcgcccg aggtcacgtg
     2401 tagttagcca tggtaggcct tctcgagcgc ggcgatgtcg agcctgccca ttttcatcat
     2461 cgcggcgaac gcgcgctgcg atttgtcgtc gggcctggtg aaccactcca ccatcttgct
     2521 cggcacgacc tgccatgaca gtccgaacct gtctttgagc cacccgcata cctgcgcctt
     2581 cgggtcaccc cccgcggtga gcttgttcca gtagtagtcg agttctttct gatcctcgac
     2641 gtagatctgc agcgaaacgg cttcgctgaa cttgaagatc ggaccgccat tcagcgccgt
     2701 gaacggcacc ccttcgattt cgaagatcac ggtcatgacc gagccctcgg gcttctggtg
     2761 cgtctcgtat ccaaccttgc cgtagcgcgc gacatcgagg attttcgagt tcttgaagac
     2821 gcttacatag aaattggcgg cttcttccgc ctggctgtcg aaccacaggc agggcgatat
     2881 tttctggctg aattgcatga gtgtgtctcc tcggtaggtg ttcactgcat agccgaacgg
     2941 gtcatcgccg gatcgacact tcgccaaaat aatggcgccc ggagaatccg gctgtcggac
     3001 agcaccggct ctatgagcgg cgcaccaacg cgtcttcgag ttcttcgagg ctgcgcccgc
     3061 gtgtttccgg caccagccgc cagatgaacg cgactcccgc cagtgcgaac acgccgtaga
     3121 tcaggaaggt attcgagctg ccgatcgtct tcatctccca cggaaagacg aactgcacga
     3181 tgacgcacac ggtcgagttg acgacgccta cacaggaaat ggcaagcccg cgaatccggt
     3241 ttggaaaaat ttccgagaac aacacccaca tgccgggccc gagcgaaagc gcgaagcttg
     3301 cgacgaagcc gagcatgccg atgagcacga tcaacgaact ctgtgcctcg ttttcgacct
     3361 ggaagccata agccgccagc aacatgaacg tcccgatgcc gaagcaacca aacatcaaca
     3421 agggacggcg cccgacacga tcgaagagca gcagcgccaa cacggtgaac acgacgttca
     3481 cgaagcctac ccacaccgtc tgcatgaatg gcgcattggc gccggcgcca gccttctcga
     3541 agatgaccac ggcgtacgac aacacggaat tgatgcccgt gatctgctgc aacacgccga
     3601 ccagcagtcc gatgatgatc actccgcgca gcgcgggact aagcacttcc gacagcgcgg
     3661 gtttcgctcg ctgcgcttcg tgttcgagtg acaccaggac tgcttcgagt tccgtatcgg
     3721 cgcgttcact gcctgtcagg cgggcgagca cactgcgcgc cgcgggcaac cggtcgtgca
     3781 tcgccagcca gcggggactc tcgggcacca tcaacatcgc gaagaaatag ggaatcgccg
     3841 gaatcgcgcc gatgcctaac atccagcgcc agctccagtc cgcgtcgcca ccgagactcg
     3901 cgatgaagag attgctgcca aaagccgcga gctgacccag gacgatgaac agctggttga
     3961 acgagaccat gcggccgcgc cacgccgacg gagaaatctc tgcgatgtac atgggcgcgg
     4021 tgacgagcac cgcaccgacg ccgaagccgc tgagcagccg cgcaacgatc agcgtggcga
     4081 aattcgccga cagcgctgcg aggagtgccg agatcaggaa gatgcaggtg gccagccgca
     4141 gtacggtgcg ccgaccgatg cgatcggcca gcggcccgga aatgaagatg gcaacggtgg
     4201 cggccacggc catcgacgtg acggcccatc cgagctcgaa ctcgttcaac tcgaattccg
     4261 actcgatgaa acccacggcg ccggtgaaca gcgaaccgtc gaaaccc
//
LOCUS       DATDEU010000498         2950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2136193, whole genome shotgun sequence.
ACCESSION   DATDEU010000498 DATDEU010000000
VERSION     DATDEU010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2136193"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /gene="fliP"
                     /locus_tag="VIV63_11925"
     CDS             <1..723
                     /gene="fliP"
                     /locus_tag="VIV63_11925"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY6125350.1"
                     /translation="LALLPLAASAAATGGAGLPAVTVTTAPSGGQTYSLTLQVLILMT
                     VLSLLPAIMLMMTAFTRIVIVLAILRQAIGAGQTPPNQVLVGLALFLTFFVMAPVIDK
                     INTEAAQPYLAGTIETTVALERAIVPLKGFMLEQTRETDIATFVRISGGKGFAKPADV
                     PLSILVPAFVTSELKTAFQIGFLLFIPFVIIDLVVASVLMSMGMMMLSPVLISLPFKI
                     MLFVLIDGWALVMGTLAQSFFR"
     gene            720..989
                     /gene="fliQ"
                     /locus_tag="VIV63_11930"
     CDS             720..989
                     /gene="fliQ"
                     /locus_tag="VIV63_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY6125351.1"
                     /translation="MTPDQVVTIGRHALETTLMLSAPLLLTALAVGLIVGIFQAATSI
                     NEMTLSFIPKLLAMAAVLAITGPWMLRTLIEYSRNLIESIPGLVG"
     gene            986..1774
                     /gene="fliR"
                     /locus_tag="VIV63_11935"
     CDS             986..1774
                     /gene="fliR"
                     /locus_tag="VIV63_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY6125352.1"
                     /translation="MSIALSIGQLEGWVAQGFFPFARIGACLMVAPVFGARFVPPRTR
                     IILAVAITALVAPMIPTPAIAPFSPQGFVVVFQQLLIGVALGFALQIVFDALGLAGQL
                     LANSMGLSFAFNVDPLRGSSTPALGQLYIILATLTFLALGGDTALIELLVAGLYALPV
                     GTTGLGQEGLWGLILWSGQLFAGAISVALPGITALLIVNLAFGVVSRAAPSLNLFAVG
                     FPVSLVVGLLVVLVGMGPVQESFTRLLAQGFDFLRSLHGANGGP"
     gene            1778..2881
                     /gene="flhB"
                     /locus_tag="VIV63_11940"
     CDS             1778..2881
                     /gene="flhB"
                     /locus_tag="VIV63_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEY6125353.1"
                     /translation="MAESSGAERTEQATPKRLEEARKKGQVPRSVELSMAAVCLAAAA
                     AIYTLGRGAAGQFADLMRGMLSLRPEQAVGEGVIWPALMDAGLRSLIIILPILGATFI
                     AALAAPLAIGGWNFSGEALMPKFSKLNTAAGLGRIFSARGSVELGKGIAKVLVVSIIG
                     WVLLSGLTPQMMGLSSEPLHGAIGHSASLAGYSLLVLCFGLVAIAAFDVPFQLWQHSR
                     DLKMTREEVKEEYKESEGSPETRGRIREAQRALARGRMMQEVPKADVVITNPTHFAVA
                     LRYDESRMRAPVVVAKGTDLVAARIREIAAENGVPLVAAPPLARALYRSVELGHEVPA
                     ALYATVAQVLTYVYQLKAAMARGAKPPPPPVIN"
BASE COUNT          418 a         1006 c          962 g          564 t
ORIGIN      
        1 ctggcgctgc tgccgctggc agcttccgcc gccgcgaccg gcggcgcggg gcttcccgcg
       61 gtgaccgtga ccacggcgcc cagtggcggc cagacctact cgctgacgct ccaggtcctg
      121 atcctgatga cggtgctgtc gctgctgccc gccatcatgc tcatgatgac ggcgttcacg
      181 cgcatcgtca tcgtgctggc gatcctgcgg caggccatcg gcgccggcca gacgccgcct
      241 aaccaggtgc tggtggggct cgcgttgttc ctgaccttct tcgtgatggc cccggtcatc
      301 gacaagataa acaccgaagc cgcgcagcct tatctcgccg gcacgatcga gaccacggtg
      361 gcgctcgagc gcgccatcgt tccgctcaag ggtttcatgc tcgagcagac gcgcgagacc
      421 gacatcgcca cgttcgtgcg catctccggc ggcaagggtt tcgcgaaacc ggccgacgtt
      481 ccgctcagca tcctggtgcc ggcgttcgtc accagcgagc tcaagacggc gttccagatc
      541 ggcttcctgc tgttcatccc gttcgtgatc atcgacctcg tggtcgcgag cgtcctcatg
      601 tcgatgggca tgatgatgct gtcgccggtg ctgatctctc tgccgttcaa gatcatgctg
      661 ttcgtgttga tcgatggatg ggcgctggtc atgggcacct tggcgcagag tttcttccga
      721 tgacacctga ccaggtcgtc accatcggcc ggcatgcgct cgaaacgacg ctcatgctct
      781 ccgcgccgct gctgctcacc gcgctcgccg tgggcctcat cgtcggcatc ttccaggcag
      841 cgacttcgat caacgaaatg acgctgtcgt tcatcccgaa gttgctcgcc atggccgcgg
      901 tgctggccat caccggtccc tggatgctcc gcacgctgat cgaatactcg cgcaatctca
      961 tcgaaagcat tccggggctg gtgggatgag catcgcgctc tccatcgggc agctcgaagg
     1021 ctgggtggcg cagggtttct ttccgttcgc gcgcatcggc gcgtgcctca tggtggcgcc
     1081 ggtgttcggc gcgcgattcg tgccgccgcg cacgcgcatc atcctcgccg tcgcgatcac
     1141 cgcgctcgtc gcgccgatga ttccgacgcc ggcgatcgcg ccgttctcgc cgcagggctt
     1201 cgtcgtcgtg ttccagcagt tgctcatcgg cgtggcgctg gggttcgcgc tgcagatcgt
     1261 gttcgacgca ctggggctcg ccggccagct tctggccaac agcatgggcc tgtcgttcgc
     1321 attcaacgtc gacccgttgc gcggcagcag cacgccggcg ctgggccagc tctacatcat
     1381 tctcgccacg ctcacgttcc tcgcgctggg cggcgacacc gcgctcatcg aattgctcgt
     1441 cgccggcctg tacgcgttgc cggtcggcac gacggggctc ggtcaggagg ggctctgggg
     1501 tctcatcctc tggagcggtc aattgttcgc gggtgccatt tccgtggcac tgcccggcat
     1561 caccgcgctg ctcatcgtga atctcgcgtt cggagtggtc agccgtgccg cgccgtcgct
     1621 gaacctgttc gcggtcggct tcccggtgtc gctggtcgtc ggtctgctgg tcgtgctcgt
     1681 cggcatgggc ccggtccagg aaagtttcac ccggttgctg gcgcagggct tcgacttcct
     1741 gcgctcgctt cacggcgcaa acggagggcc atgaaccatg gctgagtcct ccggcgcaga
     1801 acgcacagaa caagctacac cgaagcgcct cgaggaggcg cgcaagaaag gccaggtccc
     1861 gcgctccgtc gaactatcga tggcggcggt ctgcctcgcg gccgccgcgg ctatctacac
     1921 gctcgggcgt ggcgccgccg gccagttcgc cgacctcatg cgcgggatgc tgagcctgcg
     1981 gcccgagcag gcggtgggtg agggcgtgat ctggcccgcg ctcatggatg ccggcctgcg
     2041 ctcactcatc atcatcctgc cgattctggg cgccaccttc atcgcggctc tcgccgcgcc
     2101 gctcgcgatc ggcggctgga atttctccgg cgaagcgctg atgccgaaat tctccaagct
     2161 gaataccgcc gcgggcctcg gccgcatctt ctcggcgcgc ggcagcgtgg aactcggcaa
     2221 gggcatcgcc aaggtgctgg tggtctcgat catcggctgg gtgctgctct ccgggctgac
     2281 gccgcagatg atgggcctgt cgagcgagcc gctgcacggc gccatcggtc attccgcgtc
     2341 gctcgccggt tactccttgt tggtgctgtg ctttggcctg gtggcgatcg ccgccttcga
     2401 cgtgccgttc cagctctggc agcactcgcg cgatctcaag atgacgcgcg aggaagtgaa
     2461 ggaagaatac aaggagagcg aaggttctcc cgaaacacgt ggccgcatcc gcgaggcgca
     2521 gcgcgcgctg gcgcgcggcc gcatgatgca ggaagtgccg aaggcggacg tggtcatcac
     2581 caacccgacg cacttcgccg tggcgctgcg ttacgacgag tcgcgcatgc gcgcgccggt
     2641 ggtcgtggcc aagggcacgg acctggtcgc cgcgcgcatc cgcgagatcg ccgctgaaaa
     2701 cggcgtgccg ctggtggccg cgccgccgct ggcgcgcgcg ttgtatcgca gcgtcgagct
     2761 cgggcacgaa gtgccggccg cgctctacgc cactgtcgcg caggtgctca cctacgttta
     2821 ccagctcaag gccgcgatgg cgcgcggcgc caagccaccg ccgccacccg tcatcaactg
     2881 atagaacctc atggccgaca ccaacgacaa ttccccggcg agtgccttga tgcgcatggg
     2941 tatcggcgtg
//
LOCUS       DATDEU010000499         7746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_970006, whole genome shotgun sequence.
ACCESSION   DATDEU010000499 DATDEU010000000
VERSION     DATDEU010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970006"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..666
                     /locus_tag="VIV63_11945"
     CDS             244..666
                     /locus_tag="VIV63_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125354.1"
                     /translation="MDIINDRSLSRDVIERFLDRSWMLDRTPLPALNGYRFALNLLDD
                     WLTRHRVATLTNASASDVRALLKSKTWDVVSCRCDSLLGLVTRFYQSLQMSSLRDDDP
                     WDTMIVEQIAHAARKRVVRPKVREKTRRTLLLDHPEVV"
     gene            666..860
                     /locus_tag="VIV63_11950"
     CDS             666..860
                     /locus_tag="VIV63_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125355.1"
                     /translation="MAAEARHALRAAYVPAARIRLGTRTPPPTDPDDGLRASRGILTG
                     LLLGAAFWIASIAIVLFIVR"
     gene            complement(847..3381)
                     /locus_tag="VIV63_11955"
     CDS             complement(847..3381)
                     /locus_tag="VIV63_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125356.1"
                     /translation="MCALLSARAIRAGVPLIVSVLLLATPAIVEYAMAADTARDAPLS
                     SSAALALLGACLNLALVVTFVILHPRRNVWLPPTRRWRLEDMLFMASVAAMAPAAQLL
                     RADGPAADASMGWWVSSSILAVQLGIAIVSVFGSRKVRKLRLALMSGRLHAARAPAGR
                     LPGELALLFLSGRHVSDGLHRRSAIQGLHLESARLRAGRLQQQIDTHDRDRRRIDLEL
                     KQVRKFADESDRRWRALLNTVPDALFITDLKGRIEFVNEGVRALLGYVPEKLAGSQIA
                     ALVPASQPGRELPDLDAFFTAGEADATPVERNVRFVDARGVVRELAVRVQSLQIPEGA
                     HLSIRLREATGALQRGRDQFVATMSHEIRTPLHGLMATIDMLRSEALSAEGRRRLGIA
                     RTSAKTLVRIANDVLDLTRISAGGMPIERKPMSIERLVNDVVDEARERDEALPLFITA
                     EVGNEVPAAVLGDPLRIKQVLRNLVSNALKFTANGGVTVSAYWLGGQCTIDVADTGSG
                     IAPNRRESIFEAFVQADRSTSRRHGGAGLGLSIGRQLSEAMRGSLTLLQSGSRGSTFR
                     LVLPLPATDEPVQDEQSQRNLPVVKGRILVAEDDEASRYVAQTLLESLECPARIVANG
                     IEALEELRSGEFDLALLDCEMPELDGYEVTARIRKLRGRHIPIIAMTASTMSADRQRC
                     FDAGMDDLLAKPFGKSALNDMLAKWLSPQPVNSAQAPIAQEVARRPELDTSVFEELRQ
                     SLNWKIPPLQKIYRSVRESARAAIEALGGGKPKDLELAHRRLHSLQGGAGLVGARQME
                     YVAGRLLQVMKDDTHQGLAEGLAVLVEALRRFEIDLDKRLDALNAR"
     gene            3625..4491
                     /locus_tag="VIV63_11960"
     CDS             3625..4491
                     /locus_tag="VIV63_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6125357.1"
                     /translation="MPDIVLLLDDDAAALDFVEGVLTAADISCLTTNDPLQALNIVQA
                     RPDITLIISDICMPTMNGIQFADRLNSLALQWRVPSVLFLTAHPTLERAVDALRLGAH
                     DFLRKPVRPMELLEVVKRGLERAERRRQAPADSAPDVDKLIRQAERRATRPGDAAQAT
                     RGHVSVLDTIEGLHKLRLRYADHKLDELAWDLLLELLRATQHQHRLSVSALAISFPET
                     PTTTSLRRIGELTAGGYIEREPDTHDRRRDFLTLTPKARALLEDYLSCAEAFLSEARP
                     DSQALPGTSKRR"
     gene            4727..5173
                     /locus_tag="VIV63_11965"
     CDS             4727..5173
                     /locus_tag="VIV63_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY6125358.1"
                     /translation="MSITSDLESFRAESKPARLSREPQLVRAQSTSDVVQWFLDYYWI
                     RHPISNDTIAAYRSDLIALERWLTIFHDKTLLTASDQNLRAFLDSHYRTGGRRPGDVP
                     SLACIKRFYFYLVEVGLRADDPTEHVYVRMPRLAKHNLVVIAGNRN"
     gene            complement(5187..>7746)
                     /locus_tag="VIV63_11970"
     CDS             complement(5187..>7746)
                     /locus_tag="VIV63_11970"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEY6125359.1"
                     /translation="PRQVVAATFTLANDSRTAMEIESRIVLPAGWQAVVPPAPFALAP
                     GERALKLISFTIPEGAPGGDYTVRYEARDRQRPGIGDSYGLQVRVMSSTRVQVLMLEL
                     PDFAVSGDVVDGAALLRNAGNVRASVNFQVTGKFIANVSPSRGQVILEPAETRRVELK
                     IDVGRVTGRATARITFTAASAESPGDVVASGGLPLVPRASAFDALRTLDSRVELRVVG
                     RDAPAGRTSGLQPAIFGGGVLSEERDDTLSYHFRGPDRRDSGTFGASEEYWLQYKNHG
                     FQATAGDLTYGLTPLTEPGRLGRGVFLGLEGERFGASLYGMNDTFGSGDADQFGAGAR
                     MRVTPDSLLGVNFLRRGDEDDRGDIWSLRAQAFEASGVELDMEIGQSSGGGTHGGAVR
                     FALRDDRRAVRYYALGWSAGSEFEGPLRDKLYLSTGFDYPNPGSWGLRGYYRLQDWNL
                     TPLEEIDPDLRARRALSDRMQSAPTDQQVSLGTGHSIGPNATGTLDLVYRERGERESG
                     SWRAGLNRTWRNFSLAYSLEQGAGRNERSAAQFDTSLQVLSGSLRVGRSQTYGVYWMR
                     DENSELSELDGRRESAGVSASFSGVAGWSMNLDAQRSQSRFGRGAIYDIALVHQAERG
                     GRLTLCARRLEGRFARTDFMMGYSVPFAMPVTRRSDVARLRGRVFDSETAEGLRNVVL
                     RVDGAVVATNTRGEFRFPAVSVGAHQIVVEHGTDVNQVPAGNTPLAVTVSGRDAQPVM
                     IAMVRSVVIGVLVTLHPEGAEAARGAAGVLVDFRQGDTAFRRLTDAGGRVRLGGLAPG
                     TWRVSLAADTVPEGYRSEVEDLQWVLAPGETVNAEIPLTPVRRSMRMLTPLAVR"
BASE COUNT         1375 a         2746 c         2369 g         1256 t
ORIGIN      
        1 cgcaggttcc tggttgcggc gccacatatc ccgcctgcac ctgtttcgca cggcggagat
       61 caccgccgcc ggtacgcgcc gcgcaaagtc gtcgtgcaga cgcttacatg cgctacgcac
      121 taaccactgc cgccgcagac atctccattt cggtgccata tgccccttcg cgcgccggcc
      181 atcgccgtag agtcggagtc aggtagctag cgtcgggaaa gacgcgcaac ggcagaggaa
      241 cgcatggaca tcatcaacga tcgctcactg tcccgggatg tcatcgaacg atttctcgat
      301 cgaagctgga tgctcgatcg cacgccgcta ccggcgctga acggatatcg cttcgcgctc
      361 aatctgctgg atgactggct gacccggcat cgcgtcgcga cgctgaccaa tgcgtcggca
      421 tccgatgtgc gggcactgct caagtcgaag acctgggacg tggtgtcatg ccgctgcgac
      481 tccctgctgg gcctcgtcac gcgtttctac cagagcctgc agatgagcag cctgcgcgat
      541 gacgacccgt gggacaccat gatcgtcgag cagattgcgc acgccgcgcg gaagcgggtc
      601 gttcgcccaa aggtccgcga gaaaacccgt cgcaccctgc tcctcgatca cccggaagta
      661 gtttgatggc cgccgaagcc cggcacgcgc tgcgcgccgc gtacgtgccc gcagcacgga
      721 tccgcctcgg aacacgcacg ccgccgccga ccgaccccga cgatggcctg cgggcctcgc
      781 gcggcatcct cacgggcctg ctgctcggcg ccgccttctg gatagccagc atcgccattg
      841 tcctgttcat cgtgcgttga gcgcgtcgag ccgtttgtcg agatcgattt cgaatcgccg
      901 cagcgcctcg accagtaccg cgagcccctc tgccagccct tgatgcgtgt cgtccttcat
      961 cacctgcaat aacctgcccg cgacgtattc catctggcga gcccccacca gcccggcacc
     1021 tccctgcagg gaatgcagtc ggcgatgcgc gagctcgagg tccttgggct ttccgccgcc
     1081 gagcgcctcg atggccgccc gcgcggactc gcggacggaa cggtaaatct tctgcagcgg
     1141 cggaattttc cagttgagac tctggcgcaa ttcttcgaat accgacgtgt cgagctccgg
     1201 ccgccgcgcc acttcctgcg cgatcggcgc ctgggccgaa ttgaccggct gcggcgacaa
     1261 ccacttcgcc agcatgtcgt tgagcgcgga ttttccaaat ggcttggcca aaaggtcatc
     1321 catgcccgcg tcgaagcaac gctggcggtc ggcgctcatc gtgctcgcgg tcatcgcgat
     1381 gatggggatg tggcggccgc ggagcttccg gatgcgcgcg gtcacttcat aaccgtcgag
     1441 ctccggcatt tcgcaatcga gcagcgcgag gtcgaactcg ccggaacgca gctcctcgag
     1501 cgcctcgatg ccgttcgcca cgatgcgcgc cggacattcc aggctctcga gcaacgtctg
     1561 cgcgacgtaa cggctcgcct cgtcatcctc ggcgacgagg atgcggccct tcaccaccgg
     1621 caggttccgc tggctttgtt cgtcctggac cggttcgtcg gtcgccggca gcgggagcac
     1681 caggcggaac gtcgaaccgc gcgagcccga ttgcaacagc gtcaaactac cgcgcatggc
     1741 ttcgctgagc tggcgcccga tcgaaagccc gagccccgcg ccgccatgcc gccgcgacgt
     1801 cgaccggtcg gcctgcacga aggcctcgaa gatcgactcc cgccggttcg gcgcaatgcc
     1861 gctgcccgtg tcggccacat cgatggtgca ctggccgccc agccagtacg ccgagacggt
     1921 cactccgccg ttcgccgtga acttgagcgc gttggacacc aggttccgca ggacctgctt
     1981 gatgcgcagc ggatcgccga gcaccgccgc tggtacctcg tttccgacct cggccgtgat
     2041 gaagagaggc agcgcctcgt cacgctcccg cgcctcgtcg acgacgtcgt tgaccaggcg
     2101 ttcgatgctc atcggctttc gctcgatcgg catgccgccg gcgctgatgc gcgtgagatc
     2161 gagcacgtcg ttggcgatcc tgacgagcgt cttcgccgat gttcgcgcga tgccgagccg
     2221 ccgtcttccc tccgcgctca gcgcctcgct gcgaagcatg tcgatcgtcg ccatgagacc
     2281 gtgcagcggc gtgcggattt cgtggctcat cgtcgcgacg aactggtcgc gcccccgttg
     2341 cagcgcgccg gtcgcttctc gcaggcgaat gctgaggtgc gccccttcgg gaatctgcaa
     2401 cgactgtacg cgcaccgcca gttcacgaac cacgccacgc gcatccacga accgcacatt
     2461 ccgctccacg ggagtggcat ccgcttcacc cgccgtgaaa aacgcgtcga ggtccgggag
     2521 ttcgcggccc ggctgcgagg cgggcacgag cgcggcgatc tgcgagccgg ccagcttctc
     2581 gggcacgtag ccgagcaatg cgcgaacgcc ttcgttgacg aactcgatgc gccctttcag
     2641 atcggtgatg aacagcgcat cggggaccgt attcagcagc gcgcgccagc gccggtcgct
     2701 ttcgtcggcg aacttgcgca cctgcttgag ctcgagatcg atccgccgcc gatcgcgatc
     2761 gtgtgtgtct atctgctgct gcagccgccc cgcgcgaagc cgcgcagact cgaggtgcag
     2821 gccctggatc gccgagcgcc ggtggagccc gtcactcacg tgccggccgg acaggaacaa
     2881 cagcgcgagt tcgcccggca gccggcccgc gggcgctctt gcggcgtgca atcgcccgct
     2941 catgagggcg agacgcaact ttctgacctt gcggctgccg aacacactaa ctatcgcgat
     3001 tccgagctgc acggcgagaa tcgatgaaga cacccaccat cccatggagg cgtcggccgc
     3061 cgggccgtcc gcgcgcaaca actgcgcagc cggcgccatc gccgcgacgc tggccatgaa
     3121 cagcatgtcc tccaggcgcc accgacgcgt gggcggcagc cacacgttgc gtcgcgggtg
     3181 caggatcacg aaggtgacga cgagcgccag gttgagacac gcgccgagca gcgccaacgc
     3241 cgccgatgaa gacagtggcg catcgcgcgc cgtgtccgcg gccattgcgt actccacgat
     3301 cgcgggagtc gcgagcagca gaacactcac tatcaacggc acacctgcgc ggatcgcgcg
     3361 cgcgctcagc aacgcacaca tcgccgtcaa cataaccggc acaatcgcac tcgcgccaaa
     3421 cagagacgcg gcgcacagca acgacgcaca aactaccagc gcgcgccagc cgcctgtaaa
     3481 ttcgacgaac atgaaaaccg caggcaacag aaatagcgcg gagacacgcg tcccacccgc
     3541 gagcgcgaac gtcacaccgc acgcggcaac acccacaatc agtgcccgat gccggatcat
     3601 tgcggccaac gagatcacag accgatgccc gacatagttc tcctgctcga cgacgacgcg
     3661 gccgcgctcg actttgtcga aggagtgctg acggcggcgg acatatcgtg tctgacgacc
     3721 aacgacccgc tgcaggcgct caacatcgtc caggcccggc cggacatcac gctgatcatc
     3781 agcgacatct gcatgccgac gatgaacggc atccagttcg ccgaccggct caactcgctg
     3841 gccctgcaat ggcgcgtgcc gtcggtgctg ttcctgacag cgcatccgac gctcgaacgc
     3901 gccgtggacg cgttgcggct cggcgcccac gacttcctgc gcaagccggt acggccgatg
     3961 gaactgctcg aagtggtgaa gcgcggactc gaacgcgcgg agcggcgccg ccaggcgccg
     4021 gccgacagtg caccggacgt cgacaagctg atacggcaag ccgagcggcg tgcgactcgc
     4081 ccgggtgacg ccgcgcaggc aacccgcggg catgtctcgg tactcgacac catcgaggga
     4141 ctacacaaac ttcgtctccg ttacgccgac cacaaactcg acgagctggc gtgggacctg
     4201 ctgctcgaac tgcttcgagc cacgcagcat caacaccggc tgtcggtttc cgcgctggcg
     4261 atttcgtttc cggaaacgcc aacgaccaca tccttgagac gcattggcga actgacggcg
     4321 ggcgggtaca tcgagcgcga gccggataca cacgaccgca ggcgcgattt cctgactctc
     4381 actccgaagg cccgggcact cttggaagac tatctgtcgt gcgccgaagc gtttctctcg
     4441 gaagcccgcc cggattccca ggcgttgccg ggcacttcga agcgtcgctg accccctggc
     4501 gcgacttctc ctcccggcgc gacggaagat agccggtcag cggccccgca cgctctgaag
     4561 acccgcacat tccggcagaa gatagaacct ttttggacgc aacttattcg gcgaccctta
     4621 gttagtcgga ttgcgccaaa gcggggatgt ctgccgtgcg cgcatgcagc gttgcactac
     4681 gctcaatcac atgaatcgcc acgaaccaac gttcagggag ccgaacatga gcataacgtc
     4741 cgatctggaa tcattccgcg ccgagtcgaa gcccgcgcgc ctgtcccggg aaccgcaact
     4801 cgtccgtgcg cagtccacca gcgacgtcgt gcaatggttc ctggactact actggatccg
     4861 tcacccgatc tcgaacgaca cgatcgccgc gtatcgctcc gatctgatcg cgctcgaacg
     4921 ctggctcacg attttccacg acaagacgct gctcaccgcc agcgatcaga atctgcgcgc
     4981 gttccttgac tcgcactacc gcacgggcgg ccgccggccg ggtgacgtgc cgtcgctggc
     5041 ctgcatcaag cggttttact tttatctggt ggaagtgggt ctgcgtgccg acgaccccac
     5101 cgaacacgtg tatgtacgta tgccgcgtct cgcgaaacac aacctggtcg tgatcgcggg
     5161 caatcgaaac tagctaccct ccactctcag cgaaccgcca gcggcgtgag catccgcatc
     5221 gagcggcgca ctggcgtcag cggaatctcc gcgttgacag tctcaccggg cgcgaggacc
     5281 cactgcaggt cctcgacttc ggatcgatat ccttccggca cggtgtccgc cgcgagactc
     5341 acccgccagg ttcccggcgc gagcccgccg agcctgacgc gcccgcccgc atcggtgagc
     5401 cggcgaaatg cggtgtcacc ctggcggaaa tcgacgagca ctccggcggc gccgcgcgcc
     5461 gcttcggcac cctccggatg aagcgtcacg agcacgccga tgaccaccga gcggaccatc
     5521 gcgatcatca cgggctgtgc atcacgtccc gacacggtga cggcgagcgg cgtgttgccg
     5581 gccggcacct ggttcacatc cgtgccgtgc tccaccacga tctggtgcgc gccgacgctg
     5641 acggccggaa accggaattc gccgcgggtg ttggtggcga cgaccgcgcc atccacacgc
     5701 aacacgacgt tcctgagacc ttcagccgtt tccgagtcga agacgcggcc gcgcagcctc
     5761 gccacatcgc ttcggcgcgt caccggcatc gcaaacggca ccgaatagcc catcatgaaa
     5821 tcggtgcgcg cgaaccgccc ctcgaggcgg cgggcacaca acgtaagcct gccaccccgt
     5881 tcggcctggt gcacgagcgc gatgtcgtag atggcgccgc gtccgaaccg cgattggctg
     5941 cgctgcgcgt cgagattcat cgaccatccg gcgacgccag agaaacttgc ggagacaccc
     6001 gcggactcgc gccgcccatc gagttcgctc agttcgctgt tctcgtcgcg catccagtac
     6061 acgccatagg tctgcgaccg gcccacgcgc agtgatcccg acaacacctg cagcgaagta
     6121 tcgaattgcg cagcggacct ttcgttgcgc cccgctccct gctccagcga ataggccagc
     6181 gagaaattgc gccaggtacg attcagcccc gcgcgccagg acccgctttc acgctcgccc
     6241 ctctcgcggt acaccaggtc gagcgtgcca gtcgcgttcg gcccgatcga atggccggta
     6301 cccaggctca cctgctgatc cgtcggcgcg gactgcatgc gatcggacaa cgcacggcgc
     6361 gcgcgcaagt ccgggtcgat ctcctcgagc ggcgtcaggt tccagtcctg gaggcggtag
     6421 tacccgcgca ggccccagct ccccgggttc ggatagtcga agccggtaga tagatacagc
     6481 ttgtcgcgca gcgggccttc gaattcactg cctgccgacc agccgagcgc gtagtaacgc
     6541 accgcccgcc ggtcgtcgcg caatgcgaat cgaacggcgc cgccgtgagt tccgccgccg
     6601 ctgctctggc cgatctccat gtccagctcg acgccgctcg cctcgaatgc ctgcgcgcgc
     6661 aacgaccaga tgtcacctcg atcgtcctcg tctccccggc gaagaaaatt cacgccgagc
     6721 agggaatcgg gcgtgacacg catccgcgcg cctgcgccga actggtctgc atctcccgaa
     6781 ccgaacgtgt cgttcattcc atagaggctg gcgccaaaac gctcgccctc gagtcccagg
     6841 aagacgccgc gacccagtct gccgggctcg gtcagcggag tcaggccgta agtgagatca
     6901 ccggctgtcg cctggaatcc gtgattcttg tattgcagcc agtattcctc ggacgcgccg
     6961 aaggtcccgc tgtcgcgccg gtcgggaccg cgaaagtggt agctcagggt gtcgtcacgt
     7021 tcctcgctga gaactccgcc accgaagatg gcgggctgca ggcccgaggt acgccccgcg
     7081 ggtgcgtcgc ggccgacgac gcgcaactcg acgcgcgagt cgagcgtgcg cagcgcgtcg
     7141 aaggcgcttg cacgcggcac cagcggcagg cccccgctgg ctaccacgtc gccgggcgat
     7201 tccgccgatg ccgcggtgaa cgtgattcgc gccgtcgcgc gccccgtcac cctgccgaca
     7261 tcgatcttca gttccactcg ccgggtttcc gcgggctcga gaatcacctg gcccctggac
     7321 gggctgacgt tcgcgatgaa tttcccggtc acctggaagt tcaccgaggc acgcacgttg
     7381 ccggcgttgc gcaacagcgc cgcgccatcg accacgtctc cggacaccgc gaagtccggc
     7441 aactcgagca tcagcacctg cacgcgagtc gaagacatca cgcgcacctg caggccatac
     7501 gaatcgccga tgccggggcg ctgccgatcg cgcgcttcat aacgcacggt gtagtcgccg
     7561 cccggcgctc cttcgggaat cgtgaaactg atgagcttca gcgcgcgctc tccgggcgcc
     7621 agcgcgaagg gagccggagg caccacggct tgccagcccg cgggtagcac gatgcgtgac
     7681 tcgatctcca tcgccgtgcg cgaatcattc gcaagtgtga aggtggccgc gaccacctgg
     7741 cgcggt
//
LOCUS       DATDEU010000500         6029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_970061, whole genome shotgun sequence.
ACCESSION   DATDEU010000500 DATDEU010000000
VERSION     DATDEU010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970061"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VIV63_11975"
     CDS             complement(<1..1207)
                     /locus_tag="VIV63_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125360.1"
                     /translation="MSYTASDIEVLSGLEPVRRRPGMYTDTSRPNHLAHEVVDNSVDE
                     ALGGHAKEINVILHKDNSLSVADDGRGMPVDIHPKEKVSGVELILTRLHAGGKFSDKN
                     YEVSGGLHGVGVSVVNALSKQLECWIKRNGKEYNISFKDGKVSSKLEVVGSVGQRNTG
                     TTVRFWPDPKFFDSDKFSVPSLRHVLKAKAVLCPGLKITFENEATKEKDEWFFTGDLA
                     AYLKEEIGKEDALPAEPIFAKNDQPKVNVVEYAIVWTPNAERAMGESYVNLIPTAEGG
                     THVNGLRAGIAGAVREFCEFRNLVPRGIKLTPEDIFDKCCFVLSIKIQEPQFAGQMKE
                     RLASRDAASLTESFVHDSLSLWLNQHPDAGERIAQFAIENAQERTKAAQQVKRKRVTA
                     GPALPGKLAD"
     gene            complement(1275..1679)
                     /locus_tag="VIV63_11980"
     CDS             complement(1275..1679)
                     /locus_tag="VIV63_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125361.1"
                     /translation="MKDKSQIEAALERSLRRQVSVPRLDRRFDAGVWARIEAAEVRNS
                     APMIREADAPATARWLYAINVAGLASVAIFLAAMGWQMLPGLNIGESLPAISAAMSER
                     VLMSASTIIAAVAVAFGLMFTPWGRRLRDEFG"
     gene            complement(1676..2248)
                     /locus_tag="VIV63_11985"
     CDS             complement(1676..2248)
                     /locus_tag="VIV63_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6125362.1"
                     /translation="MERDADLVEALRRGDRRAVLEGLMDRYRQKVLHLALSIVREPAL
                     AEDMAQTTFVKVWQALPKFDGRASISTWLYTIARNTCLTAVTRERRMVPLEDFAEVAD
                     DDGDPSVFGTAQAGLETAGQAAAEYDVARLLEQLPEPYRRVVVLFYLEDRSCEEVGEL
                     LSMPTGTVKALLHRGRKKLAALAGETETTS"
     gene            2441..2863
                     /locus_tag="VIV63_11990"
     CDS             2441..2863
                     /locus_tag="VIV63_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125363.1"
                     /translation="MNGVFLIPITAIVMGIGIGMLGLWTDHKRKSQMLEQLHKERMAA
                     IEKGFDLPPMNADPYLEKRPANVANPARILRNGVFMVTFGIVLYFALDTVGASAAAVF
                     GLIPATIGVANLAYAAVLFDKERKAEAQNERNRLPPPL"
     gene            complement(2865..3449)
                     /locus_tag="VIV63_11995"
     CDS             complement(2865..3449)
                     /locus_tag="VIV63_11995"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY6125364.1"
                     /translation="MPKLVPGDLLSLERYSRERQEFRAQVMAHKRNRQVNVGPNTMWL
                     FEDRITVQYQVQEMLRTERIFESEGISEELSAYNPLIPDGTNWKVTFLIEYTDPEIRR
                     VQLEKLKGVEDRCWVQAAGFDRVFAIADEDMERENDVKTSAVHFLRFELTKSMIAGLK
                     GGATLSIGIDHPNYQHQISPAAENVRAALIVDLD"
     gene            complement(3458..4816)
                     /locus_tag="VIV63_12000"
     CDS             complement(3458..4816)
                     /locus_tag="VIV63_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY6125365.1"
                     /translation="MGAGDTGIREGSLQAPSRHPLDWRSADFVDEAALEKELERVFDI
                     CHGCRRCFSLCNSFPTLFDAIDASPTLELDGVDKKVYWDVVDHCYLCDMCFMTKCPYV
                     PPHPWNIDFPHLMLRAKHVQHRKGEHSFRDKLLSNTRGLGNIAGIPVIAEIVNAVNGT
                     KAGRKLLEATLGVDATAPIPKFHSTTGTTRARRETGKATQVTPSEETRGKVALFVTCY
                     GNRNEPHLPEDLTVVFEHNGIPVKVVGKEKCCGMPKLELGDLETVEKYKNFNIPELKA
                     LIDEGFDIVAPIPSCVLMFKQELPLMFPDDADVQMVKEHIFDPFEYLMLRHKAGLLRT
                     EFKEKLGKVSYHVPCHLRVQNIGLKTRDVLRLVPETTVDVIERCSGHNGTYAVKKEFR
                     AASVKIGRPVVSKVEQARADHYSSDCPMAGHQIESGLAANGAESREPEHPMTLVRKAY
                     GL"
     gene            complement(4829..5242)
                     /locus_tag="VIV63_12005"
     CDS             complement(4829..5242)
                     /locus_tag="VIV63_12005"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY6125366.1"
                     /translation="MNLKGSKTEKNLKDAFAGESQANRRYLYFAAKADVEGYNDVSTV
                     FRSTAEGETGHAHGHLEYLETVGDPATGLPIGETKLNLKAAIAGETHEYSDMYPGMAK
                     QAREEGFSEIADWFETLAKAERSHANRFQKALDAI"
     gene            5400..5675
                     /locus_tag="VIV63_12010"
     CDS             5400..5675
                     /locus_tag="VIV63_12010"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY6125367.1"
                     /translation="MKTKPPREDKLPDFEQSLLELEALVTKLEQGDVPLEAALQTFER
                     GVALTRQCQTALRTAQQKVEVLLARNGEESIEPFADDEDDTDDTGNR"
     gene            5641..>6029
                     /locus_tag="VIV63_12015"
     CDS             5641..>6029
                     /locus_tag="VIV63_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY6125368.1"
                     /translation="MKTTLMTPATAEAARPMTQSFLANRVPLFQQRIETVLARSLAID
                     GAATPRLLEAMRYSTLAGGKRVRPVLVYATGEALGAPLELLDAAAAAVELIHVYSLVH
                     DDLPAMDNDDLRRGRPTCHRAFDESTA"
BASE COUNT         1060 a         1900 c         1841 g         1228 t
ORIGIN      
        1 agtcggctaa cttcccgggc agtgcggggc cagccgtcac gcgcttgcgc ttgacctgct
       61 gcgcagcctt cgtgcgctcc tgcgcgttct cgatggcgaa ctgcgcgatg cgctcgccgg
      121 catcgggatg ctggttgagc cacaacgaca gcgagtcgtg cacgaagctt tccgtcagtg
      181 aagccgcatc gcgcgacgcg agacgctctt tcatctggcc ggcgaactgc ggttcctgga
      241 tcttgatcga cagcacgaag cagcacttgt cgaagatgtc ttcgggcgtg agcttgatgc
      301 cgcgcggcac gaggttgcgg aattcgcaga attctcttac cgcgccggct ataccggcgc
      361 gcaggccgtt cacgtgtgtg ccgccctcgg ccgtcgggat gaggttgacg tagctctcgc
      421 ccatggcgcg ttcggcgttc ggcgtccaca cgatcgcgta ttcgacgacg ttgaccttgg
      481 gctgatcgtt cttcgcgaag atgggttcgg ccggaagcgc atcttccttg ccgatttctt
      541 ccttcagata cgcggcgaga tcacccgtga agaaccactc gtctttctct ttggtggctt
      601 cgttctcgaa cgtgatcttg agaccggggc acaacaccgc cttggccttg agcacgtgcc
      661 gcagcgaagg caccgagaac ttgtcgctgt cgaaaaactt cggatccggc cagaagcgaa
      721 ccgtggtgcc ggtgttgcgc tggccgacgc tgccgaccac ttcgagcttc gaggagactt
      781 tgccgtcctt gaagctgatg ttgtattcct tgccgttgcg cttgatccag cactcgagct
      841 gcttcgacaa cgcattgacg accgacacgc cgacgccgtg cagaccgccg gagacttcgt
      901 aattcttgtc gctgaatttt ccgccagcgt gcaggcgcgt gaggatgagc tcgacgccgc
      961 tcaccttttc tttcgggtgg atgtccaccg gcattccgcg accgtcgtcc gccacggaca
     1021 atgaattgtc cttgtgaagg atgacgttga tttccttggc gtgtccgcca agggcttcgt
     1081 cgactgagtt gtcgacgact tcatgcgcga ggtggttcgg gcgtgaagtg tcggtgtaca
     1141 tgcccggccg tcggcgcacg ggctcgagac cggataagac ttcgatgtcg gatgcggtgt
     1201 aggacattct tgcgtgataa acctcgggga gcgcgcggcc cgtcagtgct gcgcggcgcg
     1261 aagtctacca gcgatcagcc gaattcgtcg cgcagtcgcc gtccccaggg cgtgaacatg
     1321 agtccgaatg ccaccgccac cgcggcgatg atcgtgctag cgctcatgag aacgcgctca
     1381 ctcatggcgg cggaaatcgc cggcagggat tcaccgatat tcaatcccgg cagcatctgc
     1441 caacccatcg cagccaggaa gatggcgacg ctcgcgagcc ctgcgacgtt gatggcgtac
     1501 aaccagcgtg ccgtcgccgg cgcatcagcc tcccgaatca tgggcgcgga gttacgtacc
     1561 tcggcggctt cgattcgcgc ccagacgccg gcatcgaacc gccgatcgag acggggtacc
     1621 gaaacctgcc tgcgcagcga gcgctcgagc gcggcttcga tttgcgactt gtctttcatg
     1681 atgtggtctc cgtttccccc gcgagcgcgg cgagtttttt ccgaccccga tgcagcaatg
     1741 ctttcacggt accggttggc atcgacagaa gctcgccgac ttcctcacaa cttcgatctt
     1801 cgagatagaa caggacgacg acgcggcgat agggctccgg cagttgctcg aggagcctcg
     1861 cgacatcgta ttccgccgcc gcctggcctg ccgtttccaa cccggcttgc gcggttccga
     1921 acaccgacgg gtcgccgtca tcgtcggcga cttcggcaaa atcctccagc ggcaccatgc
     1981 ggcgctcgcg ggtcaccgcg gtcaggcagg tattgcgcgc gatcgtgtac agccaggtcg
     2041 atatggacgc gcggccgtcg aatttgggca gcgcctgcca gaccttgacg aaggtggtct
     2101 gggccatgtc ctcggccagg gcaggctcgc gcacgatgga cagcgccagg tgcaggacct
     2161 tttgacgata acggtccatg agcccctcga gcaccgcccg acggtcgcca cgacgcaacg
     2221 cctcgacgag gtcggcgtcg cgttccatgg cgggggtctg tttgggtgcc tggggcgcaa
     2281 gcccatcgtc aatgttcata tttaccgctg gctgcacggc cttgatactc cgccggggcc
     2341 ctggtggttg caaacctcgg tatcgcatcc ctcgatgcaa ccgaacgggc tgctggcggg
     2401 tttcaaaggc gtacaaaacg cctaactgga gaagttgatc atgaacgggg tttttctcat
     2461 tcccatcacc gccatcgtca tgggcatcgg catcggaatg ctgggcctgt ggaccgacca
     2521 caagcgcaaa tcgcagatgc tcgagcagct gcacaaggag cgcatggccg ccatcgaaaa
     2581 gggtttcgat ctgccgccga tgaacgccga tccctatctc gagaagcggc ccgccaatgt
     2641 ggccaacccg gcgcgcatcc tgcgcaacgg tgtgttcatg gtgaccttcg gcatcgtgct
     2701 gtatttcgcg ctggacaccg tcggtgcgag cgccgccgcc gtcttcggcc tgatcccggc
     2761 cactatcgga gtcgcgaacc tggcctatgc ggccgtgttg ttcgacaagg aacgcaaggc
     2821 cgaagcgcag aacgaaagga atcggttgcc gccgcccctc tgattcagtc caggtcgacg
     2881 atcagcgcgg cccggacgtt ctccgccgcg ggcgagatct ggtgctgata gttagggtgg
     2941 tctatgccga tggacaatgt cgcgccaccc ttgagtcccg cgatcatcga cttggtcaat
     3001 tcgaaacgca gaaaatgaac ggctgaggtc ttcacgtcgt tctcccgctc catgtcttcg
     3061 tcggcgatgg cgaacacgcg atcgaagccg gccgcctgga cccagcaccg gtcttcgaca
     3121 cccttcagtt tctcgagctg cacgcgccgg atttccggat ccgtgtactc gatgaggaac
     3181 gtgaccttcc agttggtccc gtcggggatc agcgggttgt acgcggagag ttcttccgaa
     3241 atgccttcgg actcgaagat gcgctcggtg cgcaacatct cctgaacctg gtactgcacc
     3301 gtgatccggt cctcgaacag ccacatggtg ttcggaccca cgttcacctg gcgattgcgc
     3361 ttgtgcgcca tcacctgcgc gcgaaactcc tgccgctcgc gcgagtaacg ctccagcgac
     3421 agcagatccc ccgggaccag tttgggcatg gtcggcatta cagtccgtaa gccttgcgca
     3481 ccagcgtcat gggatgttcg ggctcgcgag actcggcacc attcgccgcg agtccgcttt
     3541 cgatctgatg gcccgccatc gggcagtcgc tcgaatagtg gtcggccctg gcctgctcca
     3601 ccttcgatac caccggccgg ccgatcttca ccgacgccgc gcggaattcc ttcttcacgg
     3661 cataggtgcc gttgtggccc gagcaccgct cgatgacatc aacggtggtt tccggcacca
     3721 gccggagcac gtcgcgtgtc ttgagtccga tgttctggac gcgcaggtgg catggcacgt
     3781 ggtagctcac cttgcctaac ttctctttga actccgtgcg caacagcccg gccttgtgac
     3841 gcagcatgag gtattcgaac ggatcgaaga tgtgttcctt gaccatctgc acgtccgcgt
     3901 cgtccgggaa catgagcgga agctcctgct tgaacatgag cacgcaggat ggaatgggcg
     3961 cgacgatgtc gaaaccttcg tcgatgagcg ccttcagctc ggggatgttg aagttcttgt
     4021 acttctccac cgtctcgaga tcgccgagct cgagcttggg catgccacag catttttcct
     4081 tgcccaccac cttcaccgga atgccgttgt gctcgaacac cacggtcagg tcttcgggca
     4141 ggtgcggctc attgcgattg ccgtagcagg tgacgaacaa cgcgaccttg ccgcgtgttt
     4201 cttcggacgg cgtcacctgc gtggccttgc ccgtctcgcg gcgggcgcgc gtcgtgccgg
     4261 tggtcgagtg aaatttcggg atgggcgccg tcgcgtccac accgagcgtg gcttccagca
     4321 acttgcggcc agccttcgtt ccattcaccg cattgacgat ctcggcgatc accggaatgc
     4381 cggcgatgtt gccgagcccg cgcgtgttcg acagcagctt gtcgcggaac gagtgttcgc
     4441 ccttgcgatg ctgcacgtgt ttggcccgca gcatgaggtg cgggaaatcg atgttccagg
     4501 gatgcggtgg aacataggga cacttcgtca tgaaacacat gtcacacagg taacagtgat
     4561 cgacgacgtc ccagtaaacc ttcttgtcca cgccatcgag ctcgagtgtc ggactcgcgt
     4621 cgatcgcatc gaacagggtc gggaaggaat tgcacaggct gaagcagcgg cgacagccgt
     4681 ggcagatgtc gaagacgcgc tcgagttctt tctcgagcgc ggcctcgtcg acgaaatcag
     4741 cgcttcgcca gtcgagcggg tgacgcgagg gcgcctgcag gctgccctcg cgtattccgg
     4801 tatctcccgc gcccacgaag cgtggccgtc agatcgcgtc gagcgctttc tggaagcggt
     4861 tcgcgtgtga gcgttccgcc ttcgcgagtg tttcgaacca gtcggcgatt tcggagaacc
     4921 cttcttcgcg cgcctgcttc gccatgcccg gatacatgtc gctgtattcg tgcgtttcgc
     4981 cagcgatggc ggccttgaga ttcaacttgg tctcgccgat cggcagaccg gtggccggat
     5041 cgcccacggt ttcgaggtac tcgaggtgac catgagcgtg gcccgtctcg ccttcagcgg
     5101 tggaacggaa cacggtcgac acgtcgttgt atccctcgac gtccgccttg gctgcgaaat
     5161 agaggtaacg gcgattcgcc tgcgactcgc cagcgaatgc gtccttcaga ttcttttcag
     5221 tcttgctgcc cttgaggttc acggctctct ccttcagtta ttcagtcgag aattactctc
     5281 atttagactt gttctaagag tggccgcact ctacggccgc gcgcgatcac gcgtcaatcg
     5341 gggcacgtgt ctgctcgcgc ggtttgaatc gacggcatct aacgtttaac gtcgcgcgcg
     5401 tgaaaacaaa accccccagg gaagacaagc tccccgactt cgaacagtcg ctgttagaac
     5461 tcgaagccct cgtgacgaag ctcgagcaag gcgacgtacc actcgaagca gcgctgcaga
     5521 ccttcgagcg cggcgtcgct ctcacgcgtc agtgccagac cgcgttgcgc accgcgcagc
     5581 agaaggtcga agtgcttctg gcccgcaacg gtgaagaatc catcgagccg ttcgcggacg
     5641 atgaagacga cactgatgac accggcaacc gctgaagccg cgcggccgat gacgcagtct
     5701 ttcctggcga accgggttcc gctattccag cagcgcatcg aaaccgtgct cgcgcgttcg
     5761 ctggcgatcg atggcgcagc gacgccgcgg ctgctcgaag ccatgcggta cagcacgctc
     5821 gccggcggca agcgcgtgcg tccggtgctc gtgtacgcca cgggtgaagc gctcggcgca
     5881 ccgctggagc tgctcgacgc cgccgccgct gccgtggagc tcatccatgt ctacagcctg
     5941 gtgcacgacg acctgcctgc catggacaac gacgacttgc gccgcggccg tcccacctgc
     6001 catcgcgcat tcgatgaatc cacggcgat
//
LOCUS       DATDEU010000501         3392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1699570, whole genome shotgun sequence.
ACCESSION   DATDEU010000501 DATDEU010000000
VERSION     DATDEU010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699570"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..783
                     /locus_tag="VIV63_12020"
     CDS             391..783
                     /locus_tag="VIV63_12020"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY6125369.1"
                     /translation="MSYYIARTFSRPFDTVVSDVIECLKAEGFGVLTDIDVQATLKTK
                     IGAEMPKYRILGACNPGLAHEGLKLENKLGVLLPCNVIVRETADRKVEVASVDPVNAM
                     ERTGNPALAATAEGVRQRLLRALSAIAP"
     gene            complement(1038..1493)
                     /locus_tag="VIV63_12025"
     CDS             complement(1038..1493)
                     /locus_tag="VIV63_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6125370.1"
                     /translation="MKVGDICQRAVVTARPLDELLSAARVMRDKHIGYLIVVEPGIAQ
                     CGLKPVGVLTDRDLVVTVMAREADPRRLLVADVMTRELVAACEDDSLGAALAQMRRIG
                     VRRLPVIGDHGELVGVLSLDDAIDALAKELGAVAGSIRSEQVAERILRS"
     gene            1750..2334
                     /locus_tag="VIV63_12030"
     CDS             1750..2334
                     /locus_tag="VIV63_12030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY6125371.1"
                     /translation="MKKRILIVDGHPDAARVRFVHALADEYARVAEASGHTVRVVKLA
                     ELEFPWLHSAGEFAGTPPPAIVEQQERLRWADHLVIVYPLWLGSMPALLKAFFEQVMR
                     PGFAFAYGKGRGLPRKLLAGKSARIVVTMGMPSLFYRLYYRAHTVRSLARNILGFVGF
                     KPVRISLIGSVEGMSANKRARCIAEIASHARRGV"
     gene            complement(2354..3010)
                     /locus_tag="VIV63_12035"
     CDS             complement(2354..3010)
                     /locus_tag="VIV63_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6125372.1"
                     /translation="MFIAGHAIAWSQSVPPPTARDREFVAGAWLEPDLQHGAELYRTC
                     AACHGEDGRGTEDGEIPAIAGQHGSVLLKQLTDFRHDQRWNERMQTFTDRHHLPGPQD
                     LTDVAAYVASLPRFPPSATGIGDGSALREGAILYFQKCEGCHGPLGQGEVLRRRPRLA
                     GQHYAYLLRQLQDAAANQRPGMDRAHAELLRGIDPAQLRGVADYLSRVSPDLSSMQRR
                     "
BASE COUNT          634 a         1096 c         1081 g          581 t
ORIGIN      
        1 gttcaggcct ccgcgccttt cagggggagc cgggagtgac tggataggca gcgcccggca
       61 attctttgtg tgtgtggact cgatgcacgc gagcttactt ctatcaacgg ccgctcctgc
      121 atcctatacg gggtagacct catggggctc acaggtggtc cgggcttcgc cggcgttgcc
      181 gattccttat taagcggcgt atgtccagga atctcgaaac cagacagggc acctcggcga
      241 tccaccgcat gctgcaagcc ggcatcggcc ctcgaagcgt ttgttcatca gtgttggtcg
      301 gccgccgcga caccacatgc gtagaagccc ggacggcgct acgcagaagc tcgaatcgcg
      361 acggcaccgc ggacggccta acgtcgcggc atgagttact acatcgccag aactttctcg
      421 cgtccattcg acaccgtggt ttccgacgtc atcgaatgcc tcaaggcgga aggattcgga
      481 gtgctcacgg acatcgacgt gcaagcgacc ctcaagacca agattggcgc ggaaatgccg
      541 aaataccgga tcctcggtgc ctgcaatccg ggcctggccc acgaaggatt gaagctcgag
      601 aacaagctcg gcgtgctgct gccgtgcaac gtgatcgtgc gcgagaccgc ggaccggaaa
      661 gtcgaagtgg ccagcgtcga tccggtgaac gccatggaac gaaccggaaa tcccgcgctg
      721 gccgctaccg ccgaaggcgt acgtcaacgg ctgctgcgcg cactctcggc catcgcgccc
      781 tgactaccct ggcggcgcgt gcaggaccgc atccactttc gccgcgattt ccgcagcccg
      841 cgatggtgcg tccgccacca cggaagcctt cacttccttc ggaggctcgc gcaggtgtgc
      901 acgaggacga tggcattcca cttacctccg tcgacggcgg gcagcgcaga tatgtcgacg
      961 gccgcgccgg attcatcgaa aagcgaatcc agtaccagcc cgcaacggcg atggttgcgg
     1021 cgaacagccg ccggtgctca tgagcgcagg atgcgctcgg cgacctgttc ggatcgaatc
     1081 gaccccgcaa ccgcgcctaa ctccttcgcc agcgcatcga tggcgtcgtc gagcgaaagt
     1141 acgcccacga gctcgccgtg atcgccgatg acgggcaggc ggcgcacgcc gatgcggcgc
     1201 atctgcgcga gcgcagcacc gagcgaatcg tcttcgcagg ctgccacgag ttcgcgggtc
     1261 atcacatccg ccaccagaag ccggcgggga tcggcttcac gcgccatgac ggtcacgacg
     1321 aggtcgcgat ccgtcagcac accgaccggt ttcagtccgc actgcgcaat tccgggttcg
     1381 acgacgatca gatagccgat gtgcttgtcg cgcatcacgc gcgcggcgct cagcagctca
     1441 tcgagcggtc gcgccgtgac gacagcgcgc tggcagatat ccccgacttt catgatggct
     1501 ctcctgtgcc tgttgacgga gatgttaggc gggtgtcaca cgggcgcgcg tccgtaacat
     1561 tgcggacgtg cccgggcaag tggcccatag gtagttttgc gaaccacgcg gcgcattcta
     1621 cggatgcgac gcggacgcct tcttcgccaa gctccctgca cagacaacag acagaggggg
     1681 gccacctgtc catcgatcgc atcggcctcg ctgaatttcg agcctggtgg ctgagtggcc
     1741 gacgtgtcca tgaaaaagcg gatcctcatc gtcgacggcc atcccgacgc ggcgcgcgta
     1801 cggttcgttc atgctctcgc cgatgaatat gcccgtgtcg cggaagcctc cgggcacacg
     1861 gtacgagtcg tgaaacttgc cgagctcgag tttccgtggc tgcacagcgc cggggagttc
     1921 gccggcacgc cgccacccgc gatcgtcgag cagcaggaac ggctgcgctg ggcggatcac
     1981 ctggtcatcg tctatccact gtggctcggt tcgatgcccg cactgctgaa ggcattcttc
     2041 gagcaggtga tgcggccggg cttcgcattc gcatacggca agggccgtgg tctgccgagg
     2101 aaactgctcg ccggaaagtc cgcgcgcatc gtcgtcacga tgggcatgcc ctcgttgttc
     2161 taccggctct actaccgcgc acacaccgtg cgatccctcg cgcgcaacat tctcggattc
     2221 gtcggattca aaccggtgcg catcagcctc attggaagcg tcgaagggat gagcgcgaac
     2281 aagcgagcgc gctgcatcgc cgaaatcgcc tcccacgccc gccgcggggt gtgagcgtga
     2341 tgcaacgcgc cggtcatctg cgctgcatgg agctcaggtc cgggctcacg cgcgagagat
     2401 agtcggcaac gccgcgcagt tgcgccgggt cgatgcccct aagcaactcc gcatgtgcgc
     2461 ggtccattcc ggggcgctgg ttcgcggcgg catcctgcaa ttggcgcagc agataggcgt
     2521 aatgctgacc ggccaggcgt ggccggcgcc gcagcacttc gccctgtcct aacggaccat
     2581 ggcaaccctc gcacttctgg aagtagagga tcgcgccttc gcggagcgcg gatccgtcgc
     2641 cgatgcccgt tgccgagggc ggaaaacgcg gcaggctcgc cacgtaggcc gcaacgtcgg
     2701 tgagatcctg gggcccgggc agatgatggc ggtcggtgaa cgtctgcatg cgttcattcc
     2761 aacgctgatc gtggcggaag tcggtgagct gcttgagcaa cacgctgccg tgttgtcccg
     2821 ctatcgccgg gatttcacca tcttcggttc cgcgcccgtc ctctccgtgg caggccgcgc
     2881 aggtccgata cagctccgcc ccgtgttgca gatcgggctc gagccacgca ccggccacga
     2941 attcgcgatc gcgggcggtg ggcggcggaa cggattgcga ccaggcgatg gcgtgacccg
     3001 caatgaacac gaggaggatc agacgagtgt acgtacgcgt ggaaaaaact tagcagaagc
     3061 ggcggcggcg cgcgtacgta gagtcaccga tggcgctaag cgcacctgcg aatcgtcccg
     3121 cggcgcgcgc gggagtagct tcacctcaag tccaggccga agaagacttt cgagatcaaa
     3181 gtccggtagc ggagcaacgc aaccgtcctt cgcgcatcga gcgacgggcc ggaggaaata
     3241 tgaaaacaat ccgccgcatc ctgattgccg tgaagaaccc cgacggacgc cggcagcccg
     3301 gcgtcgacaa ggcgatcgac atcgccagaa agctgggcgc gtcggtcgaa ctattccatg
     3361 ccatctcaac gccggtgttc ctcgagctcg aa
//
LOCUS       DATDEU010000502         2517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1554222, whole genome shotgun sequence.
ACCESSION   DATDEU010000502 DATDEU010000000
VERSION     DATDEU010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554222"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VIV63_12040"
     CDS             complement(<1..1118)
                     /locus_tag="VIV63_12040"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY6125373.1"
                     /translation="MTELEDDRDDAAGDSGGEGLAAVVGLSALALTACGGGGGGSSTP
                     PPSQNPPPVTPPVTPPVTPPVTITDAEAARFLLQAQFAVTDADLTAVKASGYAAWLGS
                     NYNAAPGQTGVAWLDSRGHNSITDEQRYFWPQFGDFMIWNQLLAGPDQMRKRFAFALS
                     EFFVVSLSPIDGFYPPYVIAGYWDVLCANAYGNFRTLLEQITLNAGMGFFLNTKGNLK
                     EDANGRQPDENYAREVMQLFTIGLYELNADGSLRLDANNNPIETYGQSDITNLARVFT
                     GYDWDYLSNGGTFTNVAWHDYDVPNTRFATNTMRFIANNHSTQAVTFLGTTIPANTAG
                     PEALRIALDRLFNHANTGPFFARQMIHCFLAGICWAFV"
     gene            1200..1841
                     /locus_tag="VIV63_12045"
     CDS             1200..1841
                     /locus_tag="VIV63_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY6125374.1"
                     /translation="MKRPRGRPPHADLLTPSEWRTVHAIQHGMTNAEIARRRGVSVNA
                     VKFHVANALAKLNLRNRRELRAWFRAPRDSALSDRGRTTMEQPSKLGPIGQIARSVGD
                     IQVSEPWYRDVLGLPHLYTFGTLAFFNCGGTRLMLSQEGGAVKESILYLRVPDIVAAH
                     AGLAARGVKFTHAPHLIHRHADGTEEWMAFFEDPDGRPLALMCQIGASAGQNR"
     gene            1847..2332
                     /locus_tag="VIV63_12050"
     CDS             1847..2332
                     /locus_tag="VIV63_12050"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6125375.1"
                     /translation="MLVRYRESPTAANRDTVLLHAGSAIYAARRASHRRELFMSATNE
                     RMSVFGPTLRFKGELKAQEDIKIEGRIEGSIHHQQRIVVGAKGEVVATVNAAQIDVEG
                     KVVGDMSAKKSIKVNQSAIVRGNLRAPSVSITEGANFNGGVTMESNAGSQGSSTPGAS
                     R"
BASE COUNT          499 a          774 c          806 g          438 t
ORIGIN      
        1 aacacaaaag cccagcatat acctgccaaa aagcaatgga tcatctgccg cgcgaagaac
       61 gggcccgtgt tcgcgtgatt gaacaggcga tcgagcgcga tgcgcagtgc ttcggggccg
      121 gcggtattcg ccgggatcgt cgtgccaagg aaagtcaccg cctgcgtcga gtgattattg
      181 gcaatgaaac gcatcgtgtt cgtggcgaag cgcgtgttgg gcacgtcgta gtcgtgccac
      241 gccacgttgg tgaacgtgcc gccattcgag aggtaatccc agtcgtaacc cgtgaacacg
      301 cgcgccaggt tggtgatgtc gctctggccg taggtctcga tcggattgtt gttggcgtcg
      361 agccgcagcg agccatcggc gttcagttcg tacagcccga tggtgaagag ctgcatgacc
      421 tcgcgcgcgt agttctcgtc gggctgacgg ccgttcgcat cttccttcag gtttcccttc
      481 gtgttcagga agaaacccat gcccgcattc agcgtgatct gctcgagcag cgtccggaag
      541 ttgccgtacg cattggcgca caacacgtcc caatagcccg cgatcacata cgggggatag
      601 aacccgtcga tcgggctcag cgacaccacg aagaattccg acagcgcgaa cgcgaaacgc
      661 ttgcgcatct ggtcgggacc ggccagcagc tggttccaga tcatgaaatc gccgaactgc
      721 ggccagaagt agcgctgttc gtcggtgatg gagttgtgcc cgcgcgagtc gagccaggcg
      781 acgccggtct gacccggtgc ggcgttgtag ttagagccga gccacgcggc ataaccgctg
      841 gccttcacgg ccgtcagatc cgcatcggtg accgcaaact gcgcctgcag caggaaacgc
      901 gcggcttcgg catcggtgat ggtgaccggt ggggtgacgg gaggcgtcac gggcggcgtg
      961 accggtggcg ggttctgcga ggggggcggc gtcgagcttc cgccgccgcc tccgccacac
     1021 gcggtaagcg ccagtgcgga cagacctaca actgcagcca gcccttcacc gccggaatcg
     1081 ccggcagcat cgtcgcgatc gtcttccagt tccgtcacga gaccccctcg tttggcgggc
     1141 cacggtacct taagtcgccg ggtagggtaa atctgtgacg gaaaccctag gattcgctca
     1201 tgaagcgccc gcgcggccgc cctccgcatg ccgacctgct gaccccgtcg gagtggcgca
     1261 ccgtgcatgc gattcagcac ggcatgacaa acgccgagat tgcgcgccgg cgcggcgtga
     1321 gcgtgaacgc ggtgaaattt cacgtggcca atgcgctggc gaagctgaat ctgcgcaatc
     1381 ggcgcgagct gcgtgcgtgg tttcgcgcac cccgtgacag cgcgctttcc gatcgaggga
     1441 ggacgaccat ggagcaacca tcgaaactcg gcccgatcgg gcagatcgcg aggtcggtcg
     1501 gcgacatcca ggtctcggaa ccgtggtatc gcgacgtgtt agggctgccg cacctctaca
     1561 ccttcggcac gctggccttt ttcaattgcg gtggcacccg gctcatgctg agtcaggaag
     1621 gcggcgccgt gaaggaatcc atcctgtacc tgcgcgtgcc ggacatcgtg gcggcccatg
     1681 cggggcttgc ggcgcgcggc gtgaagttca cgcacgcgcc ccacctgatc caccggcatg
     1741 ccgacgggac cgaggagtgg atggccttct tcgaggaccc cgatgggcgt cctttggccc
     1801 tgatgtgcca aatcggtgcc tcggcaggcc aaaaccgtta gacatattgt tagttcgcta
     1861 cagggaatcg cccacggctg cgaatcgcga cacggtttta cttcatgcag gcagcgcaat
     1921 ctatgccgcg cgtcgcgcca gccatcgcag ggagttattc atgagtgcaa cgaacgaacg
     1981 catgtcggtt ttcggcccga cgctgcgctt caagggcgag ctcaaagccc aggaagacat
     2041 caagatcgaa ggccgtattg aaggctcgat ccaccaccag caacgcattg tcgttggagc
     2101 caaaggcgaa gtcgtcgcga cggtgaatgc cgcgcagatc gacgtcgaag gcaaagtggt
     2161 tggcgacatg agcgccaaga agagcatcaa ggtcaatcag tcggcgatcg tgcgcggcaa
     2221 tctccgcgcc ccgagcgtca gcatcacaga gggagcgaat ttcaatggcg gagtcaccat
     2281 ggagtcgaat gctgggtcgc aaggatcctc cacccccggc gcgagccgct gagccggaga
     2341 agaccatgag caagaatctc tccgacaagc ccatgtcgcc cccgcccgcc ccgcagaaag
     2401 aagtcgccac cggcacctgc gtgctcggcc cgacgctgca tttcaagggc gagctctacg
     2461 gtgaggaaga cctcgaattc cagggccagc tcgaaggttc gatcgaacac acgcgca
//
LOCUS       DATDEU010000503         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_826096, whole genome shotgun sequence.
ACCESSION   DATDEU010000503 DATDEU010000000
VERSION     DATDEU010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826096"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VIV63_12055"
     CDS             <1..1284
                     /locus_tag="VIV63_12055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY6125376.1"
                     /translation="TGYRPHADLAANILPGYDFIGDTFVANDGNGRDSDASDPGDWIN
                     AGECAPGDPAAFEASSWHGTHVAGTIAALTNNSSGVAGVAFNAKVVPARVLGKCGGYT
                     SDIADAIVWTSGGTVSGVPANANPAKVLSISLGGGGACGTTTQNAINSARSRGASVVV
                     AAGNSNANASNFSPANCTGVVTVAAVGRNGGKASYSNFGATVEVAAPGGSGGANSVLS
                     TLNSGSTGPGADNYALYNGTSMATPHVSGVVALMLSVKPTLTPDQVTSILQSTARAFP
                     ATCSQCGSGIVNANAAVDAAIGGTTPPDPPGTIAEVEPNNSRAASQLISTDNTTVNGV
                     IGSTSDNDYYRLSLPAGRTLVVTLTPNASSDYDLYVYNSNGSLIGSSELGTGAVDTVT
                     VSNTGASALTRYVRVLRYSGGTGATNGKYTVNMNW"
     gene            complement(1357..2235)
                     /locus_tag="VIV63_12060"
     CDS             complement(1357..2235)
                     /locus_tag="VIV63_12060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY6125377.1"
                     /translation="MPNYLARLVLLALCAMPAAAAADSFIAPRVSTVPGGVATFKLAG
                     TADRLPTVAFNGRPVMVVRHQDSWLAILGIGLSIEPGEYHVDVLELGGGEQKLPFTVK
                     PKTYSVQQLKVPPGQVNLSAENEARVATESAKVRAALDSFSSTAPSTLRFDQPVPGRR
                     SSSFGLRRMFNGESRNPHSGMDIAAPTGTPIKAPLAGRVVDVGSYFFNGNNVIVDHGA
                     GLLTMYCHLSKIRVEVGQELKRGEVLGDVGATGRVTGPHLHWGVSLNGAMVDPALFLP
                     PPPPAKKPAAKAPEKK"
     gene            complement(2228..>3135)
                     /gene="xseA"
                     /locus_tag="VIV63_12065"
     CDS             complement(2228..>3135)
                     /gene="xseA"
                     /locus_tag="VIV63_12065"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY6125378.1"
                     /translation="AIHDILRVLRARFPAAAVLLYPTPVQGAAAVPEIVRAIEAASRR
                     NECDVLIIARGGGSLEDLWCFNDERVARAIAACRLPTVCGVGHEVDVTIADFVADVRA
                     PTPSAAALATVPDKRGWLDMLTQLEQRFAGAMSRALRAQALAVGALTQRLQISHPGAK
                     LAQHAQRLDDLEQRMRLALRASVAGEQQRLETLSTRLWRENPRHRLEALCAHAAALRQ
                     RLVSAFTGSMTALDQRLALASRTLHAVSPLATLGRGFAVVSRVDDGTLLRDAALAPAG
                     TEIEARLARGRLRATVISHTRDEDA"
BASE COUNT          546 a         1050 c         1058 g          481 t
ORIGIN      
        1 acgggttatc gcccgcatgc cgatctcgcc gctaacatcc ttccgggcta cgacttcatc
       61 ggcgacacct tcgtcgcgaa tgacggcaac ggccgcgaca gcgacgccag cgatccgggt
      121 gactggatca acgcgggcga atgcgctccg ggcgatccgg cggcatttga ggcgagcagc
      181 tggcacggca cgcacgtggc cggcacgatc gcagcgctga ccaacaactc gagcggtgtg
      241 gccggtgttg cgttcaatgc caaagtcgta cccgcgcgag tgctgggcaa gtgcggtgga
      301 tatacctccg acatcgccga tgccatcgtg tggacttccg gcggcacggt ctccggagtt
      361 cccgcgaacg ccaatcctgc caaggtgctc agcatctcgc tgggcggcgg cggggcttgc
      421 ggtaccacca cacagaacgc catcaactcg gcgcgttcgc gcggcgcgtc agtggtcgtg
      481 gcggcgggca acagcaatgc caacgcgtcc aacttctcgc cggccaactg cacgggtgtc
      541 gtaacggtcg cggccgtcgg ccgcaacggc ggcaaggcgt cgtactcgaa cttcggcgcg
      601 acggtcgaag tggcggctcc gggcggaagt ggcggcgcca acagtgttct ctcgacgctg
      661 aactcgggat cgacgggtcc gggcgccgac aactacgcgc tttacaacgg cacgtcgatg
      721 gccacgccgc atgtgtcggg tgtggtggca ctcatgttgt cggtcaagcc gacgcttacg
      781 ccggaccagg tgacctccat cctgcaaagc acggcgcgcg cctttccggc cacttgttcg
      841 cagtgcggct cgggcatcgt gaacgccaac gctgcggtgg acgcggccat cggtggcacg
      901 acgccgccgg atccgccggg caccatcgcc gaagtcgagc ctaacaactc gcgcgcggcg
      961 tcccagctga tctcgaccga caacaccacg gtgaacggcg tgatcggcag caccagcgac
     1021 aacgactact accgtctgtc gctgccggcg ggcaggacgc tggtcgtgac gctcacgccc
     1081 aacgcgagct ccgattacga cctgtacgtc tacaacagca atggttcgct gatcggcagc
     1141 agcgagctgg gtacgggtgc ggtggatacc gtgaccgtgt cgaacacggg ggcgagtgcg
     1201 ctgacgcgtt acgtccgggt cctccgctac agcggcggta ccggcgcaac gaacggcaag
     1261 tacaccgtca acatgaactg gtaggaaatg gggacactcc ccatttgcta gcaaaagggg
     1321 acgcacctcg aaaggtgggg ccggcgaaag ccggccctac tttttctcgg gcgccttcgc
     1381 ggcgggtttc ttcgccggcg gtggcggcgg caggaagagc gccggatcca ccatcgctcc
     1441 gttcagcgac acgccccagt gaaggtgcgg tccggtcacg cggcccgtcg cgccgacgtc
     1501 acccagcact tcgccgcgtt tgagttcctg gcccacctcg acgcgaattt tcgacaggtg
     1561 gcagtacatc gtcagcagac cggcgccgtg atccacgatc acgttgttgc cgttgaagaa
     1621 gtagctgccg acatcgacca cccggcccgc gagcggcgcc ttgatgggcg tgcccgtggg
     1681 ggcggcgatg tccatgccgg aatgcggatt gcgcgactca ccattgaaca tgcgccgcag
     1741 cccgaacgag ctcgagcgcc ggcccggcac aggctgatcg aaccgcaacg tcgacggcgc
     1801 ggtcgacgag aacgaatcga gcgccgcgcg caccttggcg gattccgtcg ccacgcgtgc
     1861 ttcgttctcg gccgacagat tgacctggcc cggcggcacc ttgagctgct gaacggagta
     1921 ggtcttgggc ttcaccgtga acggaagttt ttgctcgccg ccaccgagct cgagcacatc
     1981 gacgtggtat tcgcccggtt cgatgctgag gccgatgccg agaatcgcga gccacgagtc
     2041 ctggtggcgc accaccatca ccggccggcc attgaacgcg accgtcggca gacgatcggc
     2101 cgtgcccgcg agtttgaacg tggctactcc accaggcacg gtggagacgc gaggcgcgat
     2161 gaaggaatcc gctgcagccg ccgccggcat cgcacacagg gcgagtaaca ccagccgcgc
     2221 aagatagtta ggcatcttcg tcccgggtat gcgaaatcac ggtggcgcgc agacggcccc
     2281 tggccaggcg cgcctcgatt tcagttcccg cgggcgcgag ggcggcgtcg cgcagcaggg
     2341 tgccgtcgtc gacgcgactc actaccgcga aaccccggcc gagcgtcgcg agcggactca
     2401 ccgcgtgcag cgtgcgcgaa gccagcgcga gccgctgatc gagcgccgtc atgctgccgg
     2461 tgaaagcgga gaccagccgc tggcgcagcg ccgcggcatg cgcgcacagc gcctcgagcc
     2521 gatgacgcgg attttcgcgc cacagccgcg tgctcagggt ctccagccgc tgctgctcgc
     2581 cggctacgct tgcgcgcaac gcgagacgca tgcgctgctc gaggtcgtcc agccgctggg
     2641 cgtgctgcgc cagctttgcg ccgggatgcg atatctgcag acgctgcgtc aatgcgccga
     2701 cggccagcgc ctgcgcacgc aacgcgcgac tcatggcgcc ggcaaaacgt tgttcgagct
     2761 gcgtcagcat gtcgagccag ccgcgtttgt cgggcacggt ggcgagtgcg gcagcggaag
     2821 gcgtcggcgc gcgcacgtcg gcgacgaagt cggcaatggt cacgtcgact tcgtggccca
     2881 cgccgcaaac cgtgggcagg cggcaggcgg caatcgcgcg cgccacccgt tcgtcgttga
     2941 agcaccagag atcttcgagc gaaccaccgc cgcgcgcgat gatcagcaca tcgcattcat
     3001 tgcggcgcga agccgcctcg atggcgcgaa cgatttccgg caccgctgcc gcaccctgga
     3061 cgggcgtcgg gtacagcagc actgcggcgg cggggaagcg cgcgcgcagt actcgcaata
     3121 tgtcgtggat cgcgg
//
LOCUS       DATDEU010000504         2730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1555068, whole genome shotgun sequence.
ACCESSION   DATDEU010000504 DATDEU010000000
VERSION     DATDEU010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555068"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VIV63_12070"
     CDS             complement(<1..1101)
                     /locus_tag="VIV63_12070"
                     /inference="COORDINATES: protein motif:HMM:NF036554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY6125379.1"
                     /translation="MSLPPQSGVMPTLQFILRPRLRPFALLALALVQLAAPASAGVQV
                     EVRGVGEEIRANVLAYLSFERYKDSDDLSVEFVERLQERSEREVRGALRPFGFYEPKV
                     TSEVKREGSGSEQDYRVIINITPGEAVIVEKVDVKVTGPGASERMFTLITGDLPIQTG
                     DRLTHSNYEALKGGLLRAAATNGYLDARMLRNEMRVDPQNRTAQITIEFETGERYRFG
                     ATTIKQDSIDDALIRRFVRYHENDYFDATELLRTQFALDDSQYFSTVEVLPEERDREK
                     HIVPVSIVAEPNRRHRYSYGVGYGTDTQVRGTVAWEDRRVNQRGHRFRTEAKAAATAQ
                     SLDARYIVPIGDPAIEKFQMQLAGEHERKGDID"
     gene            complement(1158..1949)
                     /locus_tag="VIV63_12075"
     CDS             complement(1158..1949)
                     /locus_tag="VIV63_12075"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY6125380.1"
                     /translation="MCLLVLAWRTHPRFRLVVAANRDEYHARPAAALAPWTDLAGVAG
                     GRDLQAGGAWFAVDDIQRIGIVTNFREFGRQRRSAPSRGGLIPAYLGATRAPGEFLQE
                     LESDAPGYAGFNLLLADRQSLWYASNRADQFARELPPGIYGLSNEFLDTPWPKLVRVR
                     ARFEDLLRTADTSAPDVLRDDLFAMLADRQTAPPDAVPPGDLSPEWARKLSAPFVLDP
                     AYGTRCSTVLTVSAPGELRITERRFDAEGLQTGQSEHLLNGHDNR"
     gene            complement(1950..>2730)
                     /gene="nudC"
                     /locus_tag="VIV63_12080"
     CDS             complement(1950..>2730)
                     /gene="nudC"
                     /locus_tag="VIV63_12080"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY6125381.1"
                     /translation="GPAAEIAFLDGGHTLVAAARDEELTLLGWFRGTRCVLVELRDPQ
                     VPLPADTELRELRPLAPLLPPDSASLLAYARALALWKARHRHCGVCGTPSLPARAGHV
                     MRCASPTCGAEFFPRLDPAIIVLVTDASGERALLGRQASWAPGRYSTIAGFVEPGESL
                     EDAVIREVDEETGVLTGDVEYVASQPWPFPSSLMLGFRAVARTHDITLRDGELEDARW
                     FTRVELTSGQATLPPAGAISARLIDAWLSAPPDAPAHRWQT"
BASE COUNT          456 a          880 c          944 g          450 t
ORIGIN      
        1 gtcgatgtcg cccttgcgct cgtgttcacc ggcgagctgc atctggaatt tctcgatggc
       61 cgggtcgccg atcggcacga tgtagcgcgc gtccagcgac tgcgcggtcg cggcggcctt
      121 cgcttccgtc cggaaccggt ggcctcgctg gttcacgcgc cggtcttccc atgcaaccgt
      181 gccgcgcacc tgggtgtcgg tgccgtaacc cacgccgtac gagtagcgat gacggcggtt
      241 cggctcggcg acgatgctga cgggcacgat gtgtttttcg cgatcacgtt cttcgggcag
      301 cacttcgacg gtggaaaagt actggctgtc gtcgagcgcg aactgcgtgc gcagcagttc
      361 ggtggcgtcg aagtaatcgt tctcgtggta gcgcacgaag cggcggatca gcgcgtcgtc
      421 gatgctgtcc tgcttgatgg tggtggcgcc gaagcgataa cgctcgccgg tttcgaattc
      481 gatggtgatc tgcgcagtgc ggttctgcgg atccacgcgc atctcgttgc ggagcatgcg
      541 tgcgtcgaga taaccgttgg tggcggcggc gcgcagcagc ccgcccttga gcgcttcgta
      601 gttggagtgc gtcaggcgat cgcccgtctg gatgggcaga tcgccggtga tcagcgtgaa
      661 catcctttca ctggcgccgg gtccggtcac cttcacgtcg accttttcga cgatcaccgc
      721 ctcgccgggc gtgatgttga tgatgacgcg gtagtcctgt tcgctgccgc tgccttcgcg
      781 cttcacctcc gaggtcacct tcggttcgta gaacccgaag ggccgaagcg cgccgcgcac
      841 ttcgcgctcg ctgcgctcct gcagacgttc gacgaactcg accgacagat cgtcggagtc
      901 cttgtatcgc tcgaacgata gataggcgag cacgttggcg cgaatctctt cgccaacgcc
      961 gcgcacttcg acctgcacgc cggcgctcgc cggagccgcg agctgcacga gggcgagcgc
     1021 gagcagcgcg aaaggcctga ggcgcgggcg aagtatgaac tgcaaggtcg gcatgacacc
     1081 gctttggggc gggaggctca agattctaat gagagtcctg cccctgcgcg cgggttccgc
     1141 gtccattcgc gacacggtca acgattgtcg tggccgttca gcaagtgttc agactgtccc
     1201 gtctgcaagc cttcggcatc gaagcgtcgc tcggtgatcc ggagctcgcc cggagccgag
     1261 acggtcagca cggtcgaaca gcgtgtgcca taagccgggt cgagcacgaa cggcgccgac
     1321 agtttgcgcg cccattccgg ggagaggtcg ccgggaggaa cggcatcggg aggcgcggtt
     1381 tgccggtcgg cgagcattgc gaagaggtcg tccctcagca catcgggagc cgaagtgtcc
     1441 gcggttcgca ggaggtcttc gaatcgggcc cgcacgcgca cgagcttggg ccacggcgta
     1501 tccaggaatt cgttggacag gccgtagatt cccggcggca gttcgcgcgc gaactggtcg
     1561 gcgcgattcg acgcgtacca aagggactgc cggtcggcca gcagcagatt gaagcccgca
     1621 tatccgggcg catccgattc gagttcctgc aggaattcgc ccggcgcgcg tgtagcgccc
     1681 aggtacgcgg ggatcaaccc gccgcgcgac ggagcgcttc gccgctggcg gccgaactcg
     1741 cggaaattgg tgacgattcc aatccgctgg atatcgtcga ccgcgaacca ggcgccgccc
     1801 gcctgcagat cgcgaccccc ggcgacaccc gccaggtcag tccacggcgc cagcgccgcg
     1861 gcaggccgcg cgtgatattc gtcgcggttc gcggcgacca ccaggcgaaa ccgcgggtgg
     1921 gtgcgccagg cgaggactaa caaacacatc taggtctgcc agcggtgggc cggagcgtcc
     1981 ggcggcgcag acagccacgc gtcgatgagc cgcgcggaga tcgcgccggc gggcggcaat
     2041 gtggcctggc ccgaggtgag ttccacgcgc gtgaaccagc gggcgtcttc gagctcgccg
     2101 tcgcgcaacg tgatgtcgtg agtgcgcgcc accgcgcgga agcccagcat caacgaggac
     2161 ggaaaaggcc agggttgtga ggccacatat tcgacgtcac cggtcagcac gccggtctcc
     2221 tcgtcgactt cgcgtatgac cgcgtcctcc aggctttcgc ccggctcgac gaagccggca
     2281 atggtggaat agcggcccgg cgcccacgaa gcctggcggc ctaacaaggc gcgttcgccg
     2341 gaagcgtcgg tgacgagcac gatgatcgcc ggatcgagac gtggaaagaa ctcggcgcca
     2401 caggttgggc ttgcgcaacg catgacatgg ccggcacgcg ccggaagact cggcgtaccg
     2461 cagactccgc aatgacggtg ccgggccttc cacaacgcca gcgcacgggc gtacgccagc
     2521 agcgacgcgg aatcgggcgg gagcaggggc gccagcggcc gcaattcacg cagctcggtg
     2581 tccgcgggca acgggacctg ggggtcgcgc agctcgacca gcacgcatcg cgtaccgcga
     2641 aaccagccta acagcgtcag ttcttcgtcc cgcgcggcgg ccacgagcgt gtgcccaccg
     2701 tcgaggaagg cgatctccgc cgccgggccg
//
LOCUS       DATDEU010000505         9626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1846606, whole genome shotgun sequence.
ACCESSION   DATDEU010000505 DATDEU010000000
VERSION     DATDEU010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846606"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VIV63_12085"
     CDS             complement(<1..726)
                     /locus_tag="VIV63_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY6125382.1"
                     /translation="MAAVGLHWAIFPITQSRVTFIFIIPAIVLATIIAGRWPGVLVAV
                     VGIVNSAAMKAPGSLMIPNSAEQVALISSAVVSAMVILVGDYYRSISRRELSDLHELH
                     ELSATLAGIPKLPDQLRLILSTFARMHAAPRGMICTCESRNLTLTVAASVGFGPESIG
                     SSCVPGLAFAERGRVVVADIETDAMVAEFREALRAAGVRSIHSTPLISRDGEILGALS
                     VHFPVPRRPSEREIRIADICARKA"
     gene            complement(890..1258)
                     /locus_tag="VIV63_12090"
     CDS             complement(890..1258)
                     /locus_tag="VIV63_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6125383.1"
                     /translation="MAMRLCWCRVCQYLASGNATVNVVFPSDAVTIDGELRDYRSIAD
                     SGNVMHRRFCPACGTHVFSESEARPHLIIVRNGALDDTDLARPGATIWTAEAPDWAWI
                     DETLPRHAGQPPPLPPSPGK"
     gene            1342..2139
                     /locus_tag="VIV63_12095"
     CDS             1342..2139
                     /locus_tag="VIV63_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY6125384.1"
                     /translation="MKLYCWNVNGIRAVVKKGTFQKFVAEHKPDILCLQETKAEKGQA
                     EIDLPAYREYWNSAEKKGYSGTAIFSKHEPVKVTNGYPDSFARKFKFADELKRDSATE
                     GRVITAEYEKFHVVTVYTPNAKDDLSRLKLRHKQWDPAFLAYCQQLEKTKPVIFCGDL
                     NVAHTELDLANPKPNKGKKGFTDEEREGFQNFVDAGFVDTFRLFTQGNGHYSWWSHFA
                     NSRSRNVGWRIDYVLVSAALKKHVKEAKIHADVLGSDHCPVSIKLDI"
     gene            2178..2519
                     /locus_tag="VIV63_12100"
     CDS             2178..2519
                     /locus_tag="VIV63_12100"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY6125385.1"
                     /translation="MGEYQSAELDSVFNALCDPTRRAILARLTDADARVTEIAADFPI
                     SLNSISKHIRMLERAGLVNRAIVGRDHVLTLNAGPLEQAQAFIEKYRRFWEGRLAALD
                     AYVTAKKRKGK"
     gene            2516..2974
                     /locus_tag="VIV63_12105"
     CDS             2516..2974
                     /locus_tag="VIV63_12105"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY6125386.1"
                     /translation="MNASVANTLPPAAVVVRRTIAASAEDLFDAWLDPEAMVQWMRPN
                     KVRRTEVRVEPRVGGSYEILMLIDSGNILHSGVYRVIDRPRRLVFTWMSGPTDSKETL
                     VTVDFVAAGARTEVIVTHEQLPESAMPSHNNGWTASLGLLEEFMDRGTVK"
     gene            2971..3780
                     /locus_tag="VIV63_12110"
     CDS             2971..3780
                     /locus_tag="VIV63_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6125387.1"
                     /translation="MTGNGLLAHKTAVVYGGGGAIGGAVARVFAREGARVFLAGRTHT
                     KLAAVARDIESAGGSAELAVLDVFDERAVHAHADAVATKTGGIDIMLNAIGISHVQGN
                     PFAETTLAEFEVPITLTARAQFIAAQAVARHMITRRRGVILTLTTPGGRIAGKGFLSN
                     GVFSAATEAFSRLLAAELGGHGIRVVCLRPDAIPDAVALSHSSEVFEGFAKRVGIPVL
                     EMLAEHGRNDTLLGRLPHLSEVAEFAAFAASDRAGAMTGAIVNLTCGSMVD"
     gene            3812..4471
                     /locus_tag="VIV63_12115"
     CDS             3812..4471
                     /locus_tag="VIV63_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6125388.1"
                     /translation="MSGICITAFKWVPPFAQGLVRDLRVRWALEEAGLPYTEKLLGTG
                     DQNSPAHRALQPFGQVPVYEEDGLTLFESGSIVMHIAQKSPVLLPADAAGRARTLTWI
                     FAALNSVEPHVQNLASIDLFFSNEEWAKLRRAGAEKIARTRLEVIAASLEGRDYLEGQ
                     FTAGDLVMTTVLRILRHTSLVKDMPVLAAYQARCEARPAFARALDAQLAHFEKNQPKA
                     A"
     gene            complement(4487..5329)
                     /locus_tag="VIV63_12120"
     CDS             complement(4487..5329)
                     /locus_tag="VIV63_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125389.1"
                     /translation="MRPDESLRGAILLLAFATGAGAQTVETREDEAVGQSINYVFAAD
                     LGSGVYDLDGRTLQIYRFTYDKELREVDEDTLGVRFVMPLTFGFFDFSPFDVLSSGIP
                     TRVDSFSVVPGFEFDHLLRNDWHLIPYVRAGANVASSTVDGWLYGVGVRLEKTGEWHG
                     WDSFVRTEFNYSGVGYRQDLPADQFGRLRQGVDLTRGTGWKWGRREFEVGMYAVIDLI
                     LDSPASPVGDERSGQSIQAEFGVTIGTRPPYKIWRWDAPRLGLGYRMAGNLSAWHFLI
                     GVPF"
     gene            complement(5391..6137)
                     /locus_tag="VIV63_12125"
     CDS             complement(5391..6137)
                     /locus_tag="VIV63_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125390.1"
                     /translation="MNPPERRRVAILGAGPTGMSVAFHLGEHSLLLERRNSLERSHDY
                     SHDLEVGAAHGDSVGSENPGADGDRPAAGSTGKTVFISCSSNSLIHVERWRLPELSQP
                     RPVESLGSPPSVRTLRPLLRGELRLGVQVVRVVPAAHLIELADGHRIVFDKLVSTLSL
                     SSTHGLVMYDLPGHVRRDESLRCWLGERDIEVVDRTVREYYGDLDEFAAGKRVAAQIG
                     EAMAEKFGKATQSKVRGVRLFEPRLVSPAS"
     gene            complement(6340..6429)
                     /locus_tag="VIV63_12130"
     tRNA            complement(6340..6429)
                     /locus_tag="VIV63_12130"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6393..6395),aa:Ser,seq:tga)
     gene            6988..>9626
                     /locus_tag="VIV63_12135"
     CDS             6988..>9626
                     /locus_tag="VIV63_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY6125391.1"
                     /translation="MSHRSGLARVLAACLAMFAALFATQLSAQTFNYGEALQKSLFFY
                     EAQRAGDLPATNRVNWRGDSAMQDGSDVGRDLTGGWYDAGDHVKFGLPMAATATMLAW
                     GIVDYRSAYTATGQLDTALDQLKWATDYFIKAHPSANELYGQVGNGGADHAWWGPAEV
                     MQMARPSYRVTTTCPGSDLAGETAAALAAASIAFRPTNATYANTLLTHARQLYTFADT
                     YRGKYSDCITDAASYYNSWSGFNDELVWGAIWMYRATNETAFLDKAQSYYANLSNQQQ
                     TTIKSYKWAHAWDDKSYGSYVLLAKLTGATQYHNDAQRWLNWWTVGGTAHGADGTRVN
                     YSPGGQAVLDQWGSLRYAANTSFAALVYSDAITDTTLKARYKEFAKRQIDYALGANPQ
                     NRSYVVGFGTNPPRNPHHRTAHSSWTDQLTFPVESRHVLYGALVGGPSSPNDGASYQD
                     DRGNFVNNEVATDYNAGFTGALARLAQEYGGAPLANFPVAETVVGNEMFVEAAINATG
                     SNFTEIKALVNNQSGWPARMGDKLSFRYYFTLEAGVTPSQISINANYNQCTAPTGPTL
                     HAGSVYYVTVNCTGTKIYPGGQQHFKKEVQFRIASSGAWDPSNDWSITGVNTTPGGTP
                     ALVQRIPLYDNGVRVFGNEPGGSTPDTQPPSVPANLRVTATTATSVSLAWNASTDDVS
                     GVTGYQVFRGATQVGTPTGLTFTDTGLTASTTYSYTVRAVDATAKVSAASAAVPATTT
                     AVTQDFTVTPSPASVSVTRGATAATTVAISRTNFTGAVTMSASGLPAGVTVAFNPSAA
                     TTGNSVTATFTASGTATIGTAAVTLTATSGTLTRTTSVSLTVNDVATQTFTIAASPGS
                     LSVARGASGATSVAITRTNFTGAV"
BASE COUNT         1812 a         3183 c         2993 g         1638 t
ORIGIN      
        1 ggccttgcgc gcacagatgt cggcaatgcg gatttcccgc tcggaaggac ggcgcggcac
       61 cgggaagtgc accgacagtg cgcccaggat ttcgccatcc cggctgatga gcggcgtcga
      121 atggatcgac cggactcccg cggcacgcaa ggcttcgcga aattccgcga ccatcgcatc
      181 tgtttcgatg tcggcgacga cgacgcgccc acgttccgcg aatgccaggc ccggcacgca
      241 cgacgagccg atggattcag ggccgaaacc gacactggcg gccaccgtca gcgtgaggtt
      301 gcgcgactcg caagtgcaga tcattccgcg gggcgcggca tgcatgcgcg cgaacgtcga
      361 caggatgagc ctgagctggt cgggtagttt ggggatgccg gcgagtgtcg ccgacaactc
      421 gtgcagttcg tgcaggtcgg acagttcgcg acgggagatc gaccggtaat agtcaccgac
      481 caggatcacc atggccgaca ccaccgcgga ggaaatcagc gccacctgct cggccgaatt
      541 cggaatcatc agcgagcccg gcgccttcat ggcggcggaa ttcacgatac cgacgacggc
      601 taccagcact ccgggccagc ggcccgcgat gatggtcgcg agcacaatgg ccgggatgat
      661 gaagatgaag gtgacgcgcg actgcgtgat cgggaagatc gcccagtgca acccgacggc
      721 ggccatggcc aggacactgg cgaatccgaa gcggcgcagc gctgtcttct cgatccaggg
      781 ccagaggcgc gccaggcgga attcttgagt gatgcgcgag atcaggggct caccttgact
      841 gcttttacag gcgcgagatt acccggtcac tgtcccgatt tcagtcccgt catttgccgg
      901 gactgggtgg cagcggcgga ggctggccgg catgccgcgg cagcgtctcg tcgatccatg
      961 cccagtcggg cgcctcggcg gtccagatgg tggcgccggg ccgggcaagg tccgtgtcat
     1021 cgagcgcccc gttgcgcacg atgatcaggt gcggacgggc ttccgattcg ctgaagacgt
     1081 gcgtgccgca ggccgggcaa aagcggcggt gcatgacgtt gccgctgtcg gcgatgctgc
     1141 gatagtcgcg cagctcgccg tcgatggtca cggcatcgct cgggaacacc acgttgacgg
     1201 tggcgttgcc cgaggccagg tactggcaga cccggcacca gcacaggcgc atggccatgg
     1261 gttcagccga gatgcgaaag cgaaccgcct tgcaaagaca tccaccggta atttccatag
     1321 gcccccattt atactcgcgt catgaaactc tattgctgga acgtcaacgg cattcgtgcc
     1381 gtcgtgaaaa agggcacctt tcagaaattc gtcgcggagc acaagcccga catcctgtgc
     1441 ctgcaggaaa ccaaggccga aaagggccag gccgaaatcg atctgccggc ttaccgcgaa
     1501 tactggaatt cggccgagaa gaaaggttat tccggcaccg ccattttttc aaaacacgaa
     1561 cccgtcaagg tcaccaacgg ttacccggac agtttcgcca ggaaattcaa gttcgccgat
     1621 gagctcaagc gcgactcggc gaccgaaggc cgcgtcatca ccgccgagta cgaaaagttt
     1681 cacgtggtga cggtgtacac accgaatgcc aaggacgacc tctcgcgcct caagctccgg
     1741 cacaagcaat gggacccggc cttcctcgcc tactgccagc aattggagaa gacgaagccg
     1801 gtgatcttct gcggcgatct gaacgtcgcg cacaccgagc tcgacctggc gaacccgaaa
     1861 cccaacaagg gcaagaaggg cttcacggac gaggaacgcg aaggtttcca gaacttcgtc
     1921 gacgccggat tcgtggatac cttccgcctc ttcacgcagg gcaacggcca ttactcctgg
     1981 tggagccatt tcgcgaattc gagatcgcgc aacgtgggat ggagaatcga ctacgtgctc
     2041 gtctccgcgg cgctcaagaa acacgtcaag gaagcgaaga tccacgccga cgtgctcggc
     2101 tcggaccact gcccggtgag catcaagctc gacatctgac ttgacacggg gccggtcggc
     2161 tccgtacatt cacccatatg ggtgaatatc aatcggccga gctggacagc gttttcaacg
     2221 ccctgtgcga tcccacgcgg cgcgccatcc tcgcgcgtct tacggacgcc gacgcgcgcg
     2281 tcaccgaaat cgccgcggac ttccccatct cgctcaattc gatctcgaag cacatccgca
     2341 tgctcgagcg cgcgggcctc gtgaatcgcg ccatcgtcgg ccgcgatcac gtgctcaccc
     2401 tgaacgccgg cccgctcgag caagcgcagg ctttcatcga gaaataccgg cgtttctggg
     2461 aaggccgtct cgccgccctc gatgcctacg tcaccgccaa gaagaggaaa ggcaaatgaa
     2521 cgcctccgtc gccaacaccc tgccgccggc cgccgtggtc gtgcgccgca cgatcgctgc
     2581 ctcggccgaa gacctgttcg atgcctggct cgaccccgaa gccatggtgc aatggatgcg
     2641 ccctaacaag gtgaggcgca cggaggtgcg cgtcgagccg cgcgtcggcg gcagctacga
     2701 gatcctcatg ttgatcgact ctggaaacat cctgcacagc ggcgtctacc gcgtcatcga
     2761 tcgcccgcgc cgcctggtat tcacgtggat gtccggcccc accgacagca aggagacgct
     2821 ggtcacggtg gacttcgtcg ccgcgggcgc gcgcaccgag gtcatcgtca cgcacgaaca
     2881 actcccggaa agcgccatgc cttcgcacaa caacggctgg accgcgagcc tcgggctgct
     2941 cgaagagttc atggaccggg gaaccgtgaa atgacaggca acggcctgct cgcgcacaag
     3001 accgccgtgg tctatggcgg cggtggcgcg atcggtggcg cggtggcgcg cgtgttcgcc
     3061 cgcgaaggcg ctcgcgtatt cctcgccggc cgcacgcaca cgaaactggc ggccgtcgcg
     3121 cgcgacatcg aatccgcggg cggcagcgcg gaactcgccg tgctcgatgt cttcgatgaa
     3181 cgcgcggtgc acgcgcacgc ggacgccgtc gccacgaaaa ccggcggcat cgacatcatg
     3241 ctcaacgcca tcggcatctc gcatgtccag ggcaatccgt tcgccgaaac gacgctcgcc
     3301 gaattcgaag tgcccatcac gctcaccgcc cgcgcgcaat tcatcgccgc gcaggcggtg
     3361 gcacgacaca tgatcacgcg ccggcgcggc gtgatcctca cgctcaccac gcccggcgga
     3421 cgtatcgccg gcaagggctt cttgtcgaat ggcgtgtttt ccgcggccac cgaggccttc
     3481 tcgcgcctgc tcgccgcgga gctcggcggc catgggatcc gcgtcgtgtg cctgcgtccc
     3541 gatgccattc ccgacgccgt ggccttgtcg cactcgagcg aggtcttcga gggcttcgcg
     3601 aagcgggtcg gcatacccgt gctcgaaatg ctcgcggagc acggccgcaa cgacaccttg
     3661 ttaggccggc tgccgcatct gtcggaagtc gcggagttcg cggccttcgc cgcctccgat
     3721 cgcgccggcg ccatgacggg cgccatcgta aacctcacct gcggatcgat ggtcgactga
     3781 cctcgcccgt gcaatcatca ggagacctct catgtccggc atctgcatca ccgctttcaa
     3841 atgggttcca ccgtttgccc agggtctcgt gcgcgacctg cgagtccgct gggcgctcga
     3901 agaagccggc ctgccctaca ccgagaagct tctcgggacg ggtgatcaga actcgcccgc
     3961 acaccgcgca ttgcagcctt tcggccaggt tccggtctac gaggaagacg gcctcacatt
     4021 gttcgagagc ggctcgatcg tcatgcacat cgcgcagaag tcgccggtgc tgttgccggc
     4081 ggacgcagcc ggccgcgcgc gaacgctcac gtggatcttc gccgcgctca attccgtgga
     4141 gcctcacgtg cagaatctcg cgagcatcga cctgttcttt tccaacgagg aatgggcgaa
     4201 gctgcggcgc gcgggcgcgg aaaagattgc caggacacgc ctcgaggtca tcgccgcgtc
     4261 gctcgaagga cgcgattatc tcgaaggtca gttcaccgcc ggcgatctgg tcatgaccac
     4321 ggtgctgcgg attctccggc acacctcgct ggtcaaggac atgcccgtgc tcgccgcgta
     4381 ccaggcgcgt tgtgaggcgc ggccggcttt cgcgcgcgca ctcgacgcgc agctcgcgca
     4441 tttcgagaag aaccagccca aggccgcctg aaatcccctt gtcggatcag aaaggaacgc
     4501 cgataaggaa gtgccaggcc gacaggttgc cagccatccg gtaacccagg ccgagtcgcg
     4561 gcgcgtccca acgccatatc ttgtatggcg gccgcgttcc aatggtcact ccgaattcag
     4621 cctggatgga ttggccggat cgctcgtcgc ccacgggcga tgccggcgaa tcgaggatca
     4681 ggtcgatgac cgcgtacatc cccacctcga attcgcgcct gccccatttc cagccggtgc
     4741 ctcgcgtgag atcgactccc tggcgcaagc gcccgaactg atcggccggc agatcctgcc
     4801 ggtaacctac gccggagtag ttgaattccg tgcgcacgaa gctgtcccaa ccgtgccatt
     4861 cgccggtttt ctcgaggcgc acgcccacgc catacaacca gccatcgacc gtgctcgacg
     4921 ccacgttggc gccggcgcgt acatagggaa tgaggtgcca gtcatttcgc agcaggtggt
     4981 cgaattcgaa acccggcacc acgctgaagc tgtcgacgcg cgtcgggatg ccgctcgaca
     5041 acacatcgaa cgggctgaaa tcgaagaagc cgaaagtgag cggcatgacg aagcgcacgc
     5101 ccagcgtgtc ctcgtccact tcgcgcaatt ccttgtcgta cgtgaagcga tagatctgca
     5161 gcgtgcggcc gtccaggtcg tacacgcccg aacctagatc cgcggcgaac acgtagttga
     5221 tggactggcc gacggcctcg tcttcgcgcg tttcgacagt ctgtgcgccg gcgcccgttg
     5281 cgaacgccag caacaggatc gcgccgcgca aactttcgtc tggccgcatg tttcaccgtg
     5341 caatcggggg acgtaacaat gccacagcac cgcgccacgc ggttagggca tcaagaggcg
     5401 ggtgatacga gtcgcggctc gaacaaccgc acgccgcgca ctttcgactg tgtggctttc
     5461 ccgaacttct cggccatcgc ctcgccgatc tgcgcggcta cgcgttttcc ggcagcgaat
     5521 tcatcgagat cgccgtagta ctcgcggacg gtccgatcta ccacttcgat gtcgcgttcg
     5581 cccagccagc agcgcaggga ttcatcgcgc cggacatgcc ccgggaggtc atacatcacg
     5641 agaccgtggg ttgaagagag tgagagcgtg ctgaccagct tgtcgaagac gatgcgatgt
     5701 ccatcggcaa gctcgatgag gtgcgctgcg ggtaccacgc gcaccacctg cacacccagg
     5761 cgcaattcgc cacgcagcag tggccgcaaa gtgcgaaccg acggcggcga gccgagcgat
     5821 tcgacgggcc tgggctgcga cagctccggc agccgccagc gctcgacgtg aatcagtgag
     5881 ttcgaactgc aggagatgaa gactgtttta ccggttgacc ccgcggccgg tctgtcgcca
     5941 tctgcaccag ggttttcact gcccacgcta tcgccatgcg cagcgcccac ttcgagatcg
     6001 tgcgagtaat catgagaacg ctccaatgag tttcgccgct cgagcagcag actgtgttcg
     6061 ccgagatgga atgccaccga catccccgtc ggccccgcgc cgaggatcgc cacccgcctg
     6121 cgttccggag gattcattcc ccgtgtcagc gagtcgacgt gacgtccgcg actttcttct
     6181 tgagcaagac ccccacgaga attcccgaga ggatcgccgc gagtgtcgcc tgcaatatgg
     6241 ctttcatggc tcgcagtgtc aggcacggcg cggtcgccga ctgtccgccg acgccgcgca
     6301 tgcctgtgag cgcgatccta tcgtttgctt ccgggtgggt ggcggagggg gtgggattcg
     6361 aacccacggt cctcgtgaga ggacgccagt tttcaagact ggtgcattca accgctctgc
     6421 cacccctccg ggggacgcgg attgcgggcg caagctactg caaagctcgc tcccggtcca
     6481 gccacgttga attcgggcgg ctgcgcagcg gcgacggcca ttccttcgct ccactcgcaa
     6541 cccgcaaatg catgttcttg acttgagtga cgactgacgt gagcagcgcg cggaccacac
     6601 tgcttattag tctgaccttg tttgttttca ttattccacg ctgccactgc gaggccagtc
     6661 gatctgcgat gacctgcaaa ggacaacccc ggctgacaag cttgtcctgt gtgtctcatc
     6721 gggtggcgtg cccgcgtcgc gcggagtttc tctgattgac aggaacgcgt cgcggcataa
     6781 gctcggtcgc ggtggcaaag gactcatggc caccgcttca gggggcatgg catatggatt
     6841 tacgcctttt tcgagtttgc acactcgtat ttcccttctc cctgattcac tagaaacacg
     6901 attcgagtac ctggcatcgg gggccgcggc gcagccacgg cattcgggtc catcgatgcc
     6961 tcatctagtt agacaagggg gatcatcatg tctcatcgtt caggtcttgc ccgcgtcctc
     7021 gcggcgtgtt tggcgatgtt tgcggcgctc ttcgcgacgc aactgtcggc ccagactttc
     7081 aactacggag aagcgctgca gaaatcgctg ttcttctacg aagcacaacg tgcgggcgac
     7141 ctgcccgcga ccaatcgcgt caactggcgc ggcgactcgg cgatgcagga cggctccgat
     7201 gtgggccgcg atctgaccgg cggctggtat gacgccggcg atcacgtcaa gttcggactg
     7261 ccgatggcgg ccaccgccac catgctcgcc tggggcatcg tcgactaccg ctccgcgtac
     7321 accgcgaccg gccagctcga cacggcgctc gatcaattga agtgggccac cgactatttc
     7381 atcaaggcgc atccgtcggc gaacgaactg tatggacagg tcggcaacgg cggcgcggat
     7441 catgcctggt ggggcccggc ggaagtcatg cagatggctc gtccttccta ccgcgtcacc
     7501 accacctgcc caggttcgga cctggccggt gaaaccgccg ccgccctggc cgcggcgtcg
     7561 atcgccttcc ggcccacgaa cgccacgtac gcgaacacac tgctcacgca tgcgcgtcag
     7621 ctctacacgt tcgcggacac ctatcgcggc aagtactccg actgcatcac cgatgccgcg
     7681 tcgtattaca actcctggag cggcttcaac gacgaactcg tctggggcgc catctggatg
     7741 tatcgcgcca cgaacgaaac cgcgttcctc gacaaggcgc agagctacta cgccaacctg
     7801 tcgaaccagc agcagacgac gatcaagtcg tacaagtggg cccacgcctg ggacgacaaa
     7861 tcctatggca gctacgtgtt gttagccaag ctcacgggcg ccacgcagta tcacaacgac
     7921 gcgcagcgct ggctcaactg gtggacggtc ggcggcacgg cgcatggtgc cgacggcacg
     7981 cgcgtcaact acagccccgg cggtcaggcc gtactcgacc agtggggttc gctgcgttac
     8041 gccgcgaaca cgtcgttcgc cgcgctggtg tactcggatg cgatcacgga caccacgctc
     8101 aaggcgcgct acaaggaatt cgcgaagcgc cagatcgact acgcgctcgg cgcaaatccg
     8161 cagaaccgca gctacgtggt gggcttcggc accaaccctc cgcgtaatcc gcaccatcgc
     8221 acggcgcata gctcgtggac ggatcagctc accttcccgg tcgagagccg tcatgtgctc
     8281 tacggcgcgc tggtcggcgg accctcgtcg ccgaacgacg gcgcgtccta ccaggacgac
     8341 cgcggcaatt tcgtgaacaa cgaagttgcc accgactaca acgccgggtt cacgggcgcg
     8401 ctcgcgcggc tcgcgcagga atacggcggt gcgccgctcg cgaacttccc ggtagcggaa
     8461 accgtggtcg gcaacgagat gttcgtcgag gcggccatca acgccacggg ttcgaacttc
     8521 acggagatca aggcgctggt gaacaaccag tcgggctggc cggcgcgcat gggcgacaag
     8581 ctctcgttcc gttactactt cacgctcgaa gccggcgtga cgcccagcca gatctcgatc
     8641 aacgccaact acaatcagtg cacggcgccc accggcccga cgctgcacgc gggaagcgtt
     8701 tattacgtca ccgtgaattg cacgggcacg aagatctatc cgggcggaca gcagcacttc
     8761 aagaaggaag tgcagttccg catcgcgagc tcgggcgcct gggatccgag caacgactgg
     8821 tccatcacgg gcgtgaacac gacgccgggc ggcacgcccg cgctcgtgca gcgtattccg
     8881 ttgtacgaca acggcgtgcg ggtgttcggc aacgagcccg gtggttcgac gcccgataca
     8941 cagccgccga gcgttcccgc gaatctgcgc gtgaccgcga cgacggccac cagcgtgtcg
     9001 ctcgcgtgga atgcctcgac ggatgatgtc tccggggtca cgggatacca ggtgttccga
     9061 ggtgcgacgc aggtgggcac gcccaccggg ctcacgttca cggataccgg gctgacggcc
     9121 agcactacct actcgtacac cgtgcgagcg gtggacgcga cggcgaaagt gtcggcggcg
     9181 agcgcggcgg tgcctgccac cacgacggcg gtgacgcagg acttcacggt cacgccctcg
     9241 cccgcctcgg tgtcggtgac acggggcgcc acggccgcga cgacggtcgc gatctcgcgc
     9301 accaacttca ccggcgcggt gacgatgagt gcctcgggac tgcccgcggg cgtcacggtc
     9361 gcgttcaatc cgtcggcggc aaccaccggc aattcggtga cggcgacgtt cacggcatcg
     9421 ggcacggcga ccatcggaac ggccgcggtt acgctgacgg cgacgtcggg cacgttgacg
     9481 cgcaccacgt ccgtttcgct cacggtgaac gacgtggcga cgcagacgtt cacgattgcg
     9541 gcctcacccg gctctttgtc ggtggcgcgc ggcgcgagtg gagccacgtc ggttgccatc
     9601 acccgcacca acttcaccgg tgcggt
//
LOCUS       DATDEU010000506         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_96244, whole genome shotgun sequence.
ACCESSION   DATDEU010000506 DATDEU010000000
VERSION     DATDEU010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96244"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VIV63_12140"
     CDS             <1..1129
                     /locus_tag="VIV63_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6125392.1"
                     /translation="GDRVKLIASLQSPGGASRMIHAMTGIPRLSIDIELPRSGGGFGR
                     RLANDFVAEAVLVAQAAKQPVKLLWTRDDDLQNDHFRPFGVQRLRAAADQDGKLTGWS
                     HRVAATSRKFRSDMTDAPDWVGTLDVDGFPAACVPNYLAEFVNVDFGLARGWWRAPLH
                     TFAAFATQSFVDEVAFALKRDPLELRLEMLGEPRDLKYEEHGGPIFSTGRLAAVMREA
                     AKRIGYGRKLPKGRGIGLACHFTFGGYAAHAIEVQAKGKDWRIERCVCVADVGQVVNP
                     AGVEAQLMGATIDGLSTAIGLEISVADGRIEQTNFNGYPLLRMPDAPLVETYVLPSEM
                     KPSGAGEMGIPSAAPALANAIFAATGKRLRNLPLRSTSQNS"
     gene            1272..>2174
                     /locus_tag="VIV63_12145"
     CDS             1272..>2174
                     /locus_tag="VIV63_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125393.1"
                     /translation="MKVTTRRAAHFLFGVLGGVMLLSILPAELAVATHAATDNGEIEG
                     RPSPTFRRICLAGPNAGQNCSSNAACPSSSCFDRNVFNITVAVLYDAPAGDLTAIQNL
                     ITAGSATLFDVTDGQAEIGHATIHNNAMSTANADFVVHPTTNDTWWQALSGHFRIGGF
                     MEVSINYINTAGANAGGFFAHEFSHLVFDVRDEYETRAAGCGAVGAAANCPLPAAANG
                     TCLMDSNGSEFCWGQGNPANATDLTGGNHDPSNVTEQSVCRANRSCWAQVNWSWPATF
                     QVPAGAPNAAANGATVGATTFTVVD"
BASE COUNT          375 a          747 c          697 g          355 t
ORIGIN      
        1 gggcgatcgc gtgaagctca tcgcctccct gcaaagtccc ggaggcgcct cgcgcatgat
       61 ccatgcgatg accggcatcc cgcgcctcag catcgacatc gagctgccgc gctcgggcgg
      121 cggcttcggc cggaggcttg cgaacgactt cgtcgccgaa gcggtgctgg tggcgcaggc
      181 cgcgaagcag cccgtcaaac ttctctggac gcgcgacgac gatctgcaga acgaccactt
      241 ccggcctttc ggtgttcagc gcctgcgcgc ggcggccgac caggacggca agctcacggg
      301 ctggtcgcat cgcgtggccg ccacgtcgcg aaagtttcgc agcgacatga ccgatgcgcc
      361 cgactgggtg ggcacgctcg atgtcgatgg ctttccggca gcctgcgtgc cgaactacct
      421 cgccgagttc gtgaacgtgg acttcggcct cgcgcgcggc tggtggcgcg cgccgctgca
      481 caccttcgcc gcgttcgcca cgcagagttt cgtcgacgag gtggccttcg cgctcaaacg
      541 cgatccgctc gagctgcggc tcgagatgtt aggggagcct cgcgacctca agtacgagga
      601 acacggcggc cccatcttca gcacgggccg gctggccgcg gtgatgcgcg aggccgcgaa
      661 acggatcgga tatggccgca agcttcccaa gggccgcggc atcggcctcg cgtgccactt
      721 caccttcggc ggctacgctg cgcatgccat cgaagtgcag gccaaaggta aagactggcg
      781 catcgaacgc tgcgtctgcg tggcggacgt ggggcaggtc gtgaaccccg ccggcgtcga
      841 ggcgcagctc atgggcgcga ccatcgacgg gctttccacg gcgattgggc tcgagatttc
      901 agtggcggac gggcgcatcg agcagacgaa cttcaacggt tacccgctgc ttcgaatgcc
      961 cgacgcgccg ctcgtggaaa catatgtgct gccgtccgaa atgaagccgt ccggcgccgg
     1021 cgagatgggc ataccgagtg ccgcacccgc gctggctaac gccatcttcg ctgccacggg
     1081 caagcgcctg cgcaatcttc cgctgcgcag cacatctcaa aatagttagc cttcccgcgc
     1141 cgctttggtg cggcgccttg cggaaataag tctcacattc gtgtgaaacg cgggccgctg
     1201 tccggctcat tgcatcgcga cgcgcggata ctctgcctcg gaacggggga cacgttcgac
     1261 agggagaggt catgaaagtc accacgcgca gggccgcgca tttcttgttt ggagtgctgg
     1321 gcggcgtgat gctgctctcc atcttgccgg cggaactcgc ggtcgcgaca cacgccgcca
     1381 ccgacaacgg cgaaatcgaa ggccgcccct caccgacatt ccgccgcatc tgccttgccg
     1441 gtcccaacgc cggtcagaac tgcagttcca acgcggcctg cccgagttcg agctgcttcg
     1501 accgcaatgt cttcaacatc accgtcgccg ttctctacga cgcgccggcg ggtgacctca
     1561 ccgcgatcca gaacctgatc acggccggct cggccacgtt gttcgacgtc acggacgggc
     1621 aggccgagat cggccacgcc accatccata acaatgcgat gagtacggcg aacgcggact
     1681 tcgtcgtcca cccgaccacc aacgacacct ggtggcaggc gctgtccgga cacttccgga
     1741 tcggcggatt catggaagtg tcgatcaact acatcaacac tgcgggcgcc aatgccggcg
     1801 ggttcttcgc gcacgagttc agccacctgg tcttcgacgt gcgcgacgaa tacgagacgc
     1861 gcgcggccgg atgcggcgcg gtcggcgccg ccgcgaattg cccgttgccc gctgccgcca
     1921 acggcacctg cctcatggac agcaacggat cggaattctg ctgggggcag ggcaatcccg
     1981 cgaacgccac cgatctcacc ggcggcaatc acgaccctag caacgtcacc gagcagtcgg
     2041 tgtgccgcgc gaatcgatct tgctgggcgc aggtgaactg gtcctggccc gccacgttcc
     2101 aggtgcccgc cggagcgccg aacgcggccg cgaacggcgc gacggttggc gccaccacct
     2161 tcaccgtggt cgac
//
LOCUS       DATDEU010000507         6212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1410259, whole genome shotgun sequence.
ACCESSION   DATDEU010000507 DATDEU010000000
VERSION     DATDEU010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410259"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VIV63_12150"
     CDS             <1..1401
                     /locus_tag="VIV63_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY6125394.1"
                     /translation="RPTPAWWGDAKFGIFIHWGVYSVPAFAPKGEYAEWYWERLDKPG
                     KSGASTEDAKIRRETRAFHERVYGKDFKYQDFAPLFTAEMFDADQWADIFARSGAKYV
                     VLVSKHHDGFALWPSREANASWGRPWNAVEVGPRRDLVGELNAAVRRRDLEMGFYFSL
                     YEWFNPRWLAGDVDAYVQGHLFPQFKDLVTRYSPSVIFSDGEWDLPSEKWRAPELLAW
                     LFNDSPVAKHVVVNDRWGGESRHKHGGYYTTEYGAGLPGATHAWEENRGLGHSYGFNR
                     NEDLADYATGPRLLLTLIDTVSRGGNLLLNVGPTSDGLIPVVMQDRLAYLGAWLKHNG
                     EAIYGTRTFRDGAQWTAGRKQEVDTSTNYRAKYDVETLTLHPATGDARKEILFTRKGN
                     VLYAILPRYPTGSLALRDLRLAKDARVSLLSSRHADIKWRQSSKDVIVSVPVIEDGEL
                     PFDGPRVLRIEGAIVE"
     gene            complement(1408..2460)
                     /locus_tag="VIV63_12155"
     CDS             complement(1408..2460)
                     /locus_tag="VIV63_12155"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HEY6125395.1"
                     /translation="MLALAAMPAAAADFYGMLPMRDLSPFGSLRLDMRPTHAVLTEPG
                     RWSLQTEFATQNTWAMSPGVEKYLTGLEGTGRRPLGPTELAAIRDLPGENYLVDLEST
                     NVDITLNYRFTSHLTGYLITSAVSYEHGFLDGTVERFHDVFSLSSYGRPAIARNGANL
                     IYDLKSDSYASLGDLPTSGGMLDPTIGLRYSGIPLGRSWSLSVEGAVKLALDGERELL
                     STGRSDYGLQAAVQWRGERQAFYANVSAVYYAGGEFLVEHETQVIPTLVVGYEYALTA
                     NTNLNIQGYASTSVYSRHDTDLDELLANKYQLTAGFRHRRDDVVISFGITENVQNINN
                     TPDIGLQLGLSWLPGN"
     gene            complement(2607..4670)
                     /locus_tag="VIV63_12160"
     CDS             complement(2607..4670)
                     /locus_tag="VIV63_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6125396.1"
                     /translation="MNSHVRRTIAAALLCALGSSLAAAPQPLDHFARRPQMQGVSISA
                     DGRYVAFLSGAGDETVLMTFDRTQPGSEFKRVTSSEPGKFDLSWCRWANPKRLLCGVS
                     GNIRGKKYADPPFARIFGVDADGSALKVLEQSRNDANLFVQTTSMRNLNMNYGAEIEK
                     SSLSGHALRGGVDFAGSAVAEKYFAGFAAARRDEVVDFTPEDTDGVLIQVDDDHDSFK
                     TIFQLNINNGFRSVRVPENPPIQNFVSDGRGNVRIGWGTSGTGNTMYYARLEGDNEWR
                     SLGAIKAFVDLNSLRPIAMSPEANAAYAIGPSEGRDAFWSIDLTDKLEPQMLFKHAVV
                     DVGDPILLTDQRLLGVRYDVERPYVWYTDPKLRELVDKLEKQYPNRAFDIIDSSADLK
                     ILVVQSFSDTDAGTYYTYDTDKNLLQKLGTAYPELDQKSLGKMTGIVYKAADGTDIPG
                     YLTIPTGAARKNLPLIVMPHDGPTQRDSWRFSFLRTFLANRGYAVLQMNYRGSSGFGQ
                     KWKYDAHQDWGGITYSDISDATRWAISEGIADPKRICIMGWGFGGYAALLGAARNSDT
                     YRCAVSIAGISDLEMQKEEAVIFGDKDFRRAQIGTDAAKLKRDSPLQNVEQIGIPVLL
                     VHGTKDWQVQVDQTRALSKALDKRKKVQRTVILDGAGHDLERQSDRVTLLKEIEAFLA
                     HTMPP"
     gene            4869..>6212
                     /locus_tag="VIV63_12165"
     CDS             4869..>6212
                     /locus_tag="VIV63_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY6125397.1"
                     /translation="MSAELKCPFHPAPGGGTSNKEWWPNQLRLDLLHQHSAKSDPMGD
                     GFDYATEFKSLDLTALKKDLAALMTDSQSWWPADFGHYGPLFIRMAWHSAGTYRTGDG
                     RGGGGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGRRISWADLMILTGNVALETM
                     GFKTFGFAGGRKDVWEPDHDVYWGKETKWLDDKRYSGDRDLENPLAAVQMGLIYVNPE
                     GPNGNPDPVAAARDIRETFARMAMNDEETVALIAGGHTFGKTHGAGPASHVGVDAEAG
                     DLEHQGFGWKSGFNTGMGVDAITSGLEVTWTTTPTKWGNDFFKHLFGYEWELTKSPAG
                     AHQWKPKGDAGAGTVPHAHDPSKRIAPSMLTTDLALKADPAYEKISRRFFEHPEQFAD
                     AFARAWFKLTHRDMGPRARYLGPEVPAEELIWQDPIPKVNHPLIDAQDIAALKGKI"
BASE COUNT         1145 a         1896 c         2004 g         1167 t
ORIGIN      
        1 cgtccgacgc cggcgtggtg gggcgatgcg aagttcggca tcttcattca ctggggcgtt
       61 tactccgtgc ctgcgtttgc gccgaaaggc gaatacgccg aatggtattg ggaacgcctc
      121 gacaagccgg gcaaatccgg cgcctcgacg gaagacgcga aaatacggcg cgagacgcgc
      181 gcctttcacg aacgcgtgta tggcaaagac ttcaaatatc aggacttcgc gccactcttc
      241 acggcggaga tgttcgacgc cgatcagtgg gcggatatct tcgcgcgctc cggcgccaag
      301 tacgtcgtgc tggtttcgaa gcatcacgat ggtttcgcgc tgtggccttc gcgcgaggcg
      361 aacgcgagct ggggccgccc gtggaacgcc gtcgaagtcg ggccgcggcg cgatctcgtc
      421 ggtgaactta acgccgccgt gcggcgccgt gacctcgaga tgggtttcta tttttcgctg
      481 tacgagtggt tcaacccgcg ttggctggcc ggcgacgtcg atgcctatgt ccaaggccac
      541 ctgttcccgc agttcaagga cctggtcacg cgttactcac cgtcggtgat attttccgat
      601 ggcgagtggg atctgccttc cgagaagtgg cgcgcgcccg aactgctcgc ctggctgttc
      661 aacgacagcc ccgttgcgaa gcacgtggtg gtcaacgatc gctggggcgg cgagtcgcgc
      721 cacaaacacg gcgggtacta caccactgaa tatggcgcgg gcttgcccgg cgcaactcac
      781 gcgtgggagg aaaaccgcgg gctcggtcat tcctatggtt tcaatcgcaa cgaagatctc
      841 gccgactacg caaccgggcc ccggctgctg ctgaccctca tcgacaccgt gagccgcggc
      901 ggcaacctgc tgctgaacgt cggcccgacg tcagatggcc tcattcccgt ggtcatgcag
      961 gacaggctgg cgtatctcgg cgcgtggctc aagcacaatg gcgaggccat ctacggcacg
     1021 cggaccttcc gcgacggtgc gcaatggacg gcggggcgca agcaggaggt ggacacgtcc
     1081 actaactatc gcgcgaagta cgacgtcgag actctcacgc tgcatcccgc gacgggcgat
     1141 gcgcgcaagg agatcctgtt cacgcgcaag ggcaacgtgc tctacgcgat cctgccgcgt
     1201 tatccgacgg gcagcctggc gttgagggac ttgcgcctgg ccaaggacgc ccgcgtgagt
     1261 ctgctgagct ctcgacacgc cgacatcaag tggcggcaat cctccaaaga cgtgatcgtc
     1321 agtgtcccgg tgatcgaaga cggcgaacta cccttcgatg gcccgcgagt tcttcggatc
     1381 gaaggtgcga ttgttgagtg aagggcgtta attacccggc agccacgaca accccagctg
     1441 caagccgatg tcgggcgtgt tgttgatgtt ctgtacgttc tcggtgatgc cgaacgagat
     1501 cacgacgtcg tcgcgccgat gccggaagcc ggcggtcagc tggtacttgt tagcgagcag
     1561 ctcatcgaga tccgtatcgt ggcgcgagta cacgctggtg ctcgcgtaac cctggatgtt
     1621 caggttggtg ttcgcggtca gcgcgtattc gtaacccacg acgagggtcg gaatgacctg
     1681 ggtttcgtgc tccaccagga attcgccgcc cgcgtagtaa accgcgctga cgttggcgta
     1741 gaacgcctga cgctcgccgc gccactgcac cgcggcctgc aggccgtagt cggaccggcc
     1801 ggtcgacaag agttcgcgct cgccgtcgag cgccagtttg acggcgcctt ccacggacag
     1861 actccacgaa cgtccgagcg ggatgcccga gtaacgcagg ccgatagtgg gatcgagcat
     1921 gccgccgctg gtcgggaggt caccgaggct ggcgtatgaa tccgacttta ggtcgtagat
     1981 caggttcgcg ccgttgcgcg cgatcgcggg tcgtccatac gaacttagcg agaacacgtc
     2041 gtgaaaacgt tcgacggtgc cgtcgaggaa gccgtgctca taggacaccg cgctcgtgat
     2101 caggtagccc gtcaggtgcg atgtgaatcg gtagttcaac gtgatgtcga cattggtcga
     2161 ctcgaggtcg acgaggtagt tctcgcccgg caggtcgcgg atcgccgcga gttccgtggg
     2221 cccgagcgga cgacgtccgg tgccttcgag gccggtcagg tatttctcga ctcccgggct
     2281 catggcccag gtgttctggg tggcgaattc ggtctggagc gaccagcggc cgggctcagt
     2341 cagcacggcg tgcgtcggcc gcatatcgag gcgcagggat ccgaaaggcg acaggtcgcg
     2401 catgggcaac atgccgtaga agtcggccgc cgccgccggc attgccgcca atgcgagcat
     2461 cgtaatgaac cccagacctg accttcgagc cttcataaat cactcctctc cttggagcta
     2521 actatgaaga atcgagcggc ccgcagcgcc ttgcactgga gaggtagggg cctccaccaa
     2581 aagaggtagg cgctgctccc gcctccttac ggcggcatgg tgtgcgcgag aaaggcctcg
     2641 atttccttca gcagcgtcac gcgatccgac tgccgttcga gatcgtgccc tgcaccatcg
     2701 aggatcaccg tcctctgcac cttcttccgc ttgtcgagcg ctttcgacag ggcccgggtc
     2761 tggtccacct ggacctgcca gtccttggtc ccgtgcacga gcagcacggg aatgccgatc
     2821 tgctcgacat tctgcagggg cgagtcgcgt ttcagtttcg cggcgtcggt cccgatctgg
     2881 gcgcggcgga agtccttgtc gccgaagatc accgcctctt ccttctgcat ctcgagatcc
     2941 gagatgccgg cgatgctgac ggcgcagcgg taggtgtcgc tgttgcgcgc agcgcctaac
     3001 aacgccgcgt agccgccgaa tccccatccc atgatgcaga tccgtttcgg gtcggcgatg
     3061 ccttcgctga tcgcccaacg cgtggcgtca ctgatgtccg agtaggtgat gccgccccaa
     3121 tcctggtgcg cgtcgtactt ccatttctgg ccgaagccgg aagagccgcg gtagttcatc
     3181 tgcagcaccg cgtagccgcg attggcgagg aacgtgcgca ggaacgagaa cctccacgaa
     3241 tctcgctgtg tcgggccgtc atgcggcatc acgatgagcg gcaggttctt gcgcgcggcg
     3301 ccggtcggaa tggtcagata acccgggatg tccgtgccgt cggccgcctt gtagacgatg
     3361 ccggtcatct tgccgagcga cttctgatcg agctcgggat aggcggtgcc taacttctgc
     3421 agcagattct tgtcggtgtc gtaggtgtag taggtgccgg cgtcggtgtc gctgaacgac
     3481 tggacgacca ggatcttcag gtccgcggaa ctgtcgatga tgtcgaaggc gcggttggga
     3541 tactgctttt ccagcttgtc gacgagttcg cgcaacttcg ggtccgtgta ccacacgtac
     3601 ggacgctcga cgtcgtagcg aacgccgagc agacgctggt cggtcagcag gatgggatcg
     3661 cccacgtcga cgaccgcgtg cttgaacaac atctgcggct cgagcttgtc ggtcaggtcg
     3721 atcgaccaga aagcatcgcg gccctcggag ggtccgatgg cgtacgcggc attcgcctcc
     3781 ggcgacatgg cgatcggccg cagcgaattc agatccacga atgccttgat ggcgccgagc
     3841 gaccgccatt cgttgtcgcc ttcgagtcgc gcgtagtaca tcgtgtttcc cgtcccggaa
     3901 gtgccccagc cgatgcgcac gttgccgcgg ccgtccgaga cgaaattctg gatgggcggg
     3961 ttctccggaa cgcgcacgct gcggaatccg ttgttgatgt tgagctggaa gatcgtcttg
     4021 aaggagtcgt gatcgtcgtc gacctggatc aggacgccat ccgtatcttc gggcgtgaag
     4081 tcgacgactt cgtcgcggcg cgcggcggcg aagccggcga agtacttttc ggccacggcg
     4141 gaaccggcga aatccacgcc gccccggagc gcgtgaccgg acagcgaact cttctcgatc
     4201 tcggcgccgt aattcatgtt gaggttgcgc atgctcgtcg tctggacgaa caggttggcg
     4261 tcattgcgcg attgttcgag caccttgagc gcggaaccgt cggcgtcgac gccgaatatg
     4321 cgagcgaacg gcggatcggc atattttttg ccgcggatgt tgcccgagac gccgcacagc
     4381 agccgcttcg ggttcgccca tcggcaccag ctgagatcga atttgccggg ttcggaggac
     4441 gtgacccgtt tgaattcgct gcccggctgg gtccggtcga acgtcatcag cacggtttcg
     4501 tcgccggcgc ccgagaggaa agcgacgtac cggccgtctg ccgagatgct cacgccctgc
     4561 atctggggtc ggcgggcaaa gtgatcgaga ggttggggcg ccgccgcgag gctggatccc
     4621 agggcgcata gcaaagcagc ggcgatcgtg cgccgcacat ggctgttcat cgtgaccccc
     4681 ttcgttgcat tgaacatcga catccgcgcg tgccgagtat ccaattcatt tgcgcgaatt
     4741 ggctagcttt atccgctagc cgaaggtcac atagacaaaa cctatcgaat tcatccaaac
     4801 aatcgaattc tgcgcgtaat cgaatgcgcg taggtttccg tcaccgttca atgagaggga
     4861 atcgaaccat gtcagcagaa ctcaaatgcc cgttccatcc cgccccgggc ggcggcactt
     4921 cgaacaagga atggtggcct aaccagttac gtctggacct gctgcaccag cactccgcga
     4981 agtccgatcc gatgggcgac ggcttcgatt acgcaacgga attcaagagc ctcgacctga
     5041 ctgcgttgaa gaaagatctc gcggcgctca tgaccgattc ccagtcctgg tggccggcgg
     5101 acttcggcca ctacggtccg ctgttcatcc gcatggcctg gcacagcgcc ggcacgtatc
     5161 gcacgggtga cggacgcggt ggtggtggcc gcggtcaaca acgtttcgcg ccgctcaaca
     5221 gctggccgga caacgtgagt ctcgacaagg cgcgccggct gctgtggccc atcaagcaga
     5281 aatatggccg caggatttcc tgggcggatc tcatgatcct cacgggcaac gttgcgctcg
     5341 agaccatggg cttcaagacg tttggtttcg ccggtggccg caaggacgtc tgggagccgg
     5401 accacgacgt gtactggggc aaggaaacca agtggctgga cgacaaacgt tacagcggtg
     5461 atcgcgacct cgagaatcca ctcgcggccg tgcagatggg actcatctat gtgaaccccg
     5521 aaggtcccaa cggcaacccc gatccggtcg cggcggcgcg cgacatccgc gagactttcg
     5581 cgcgcatggc gatgaacgac gaggaaaccg tggcgttgat cgccggcggc cacactttcg
     5641 gcaagactca cggcgcgggc cccgcgtcgc atgtcggcgt ggacgcggaa gcgggtgacc
     5701 tcgagcacca gggcttcggc tggaagagtg gattcaacac cggcatggga gtcgatgcga
     5761 tcacgagtgg tctcgaggtc acgtggacca cgacgccgac gaagtggggc aacgatttct
     5821 tcaagcacct cttcggctat gagtgggagc tcacgaagag cccggcgggc gcgcaccagt
     5881 ggaagccgaa gggcgacgcg ggcgcgggca cggtgccgca tgcacacgac ccgtcgaagc
     5941 gaatcgcgcc ttcgatgctg acgaccgatc tcgcgctgaa agccgatccg gcttacgaaa
     6001 aaatctcgcg ccgtttcttc gaacatccgg agcagttcgc cgatgcgttc gcgcgtgcct
     6061 ggttcaagct cacgcaccgc gacatgggtc cgcgcgcgcg ttatctcggc ccggaggttc
     6121 cggccgaaga actcatctgg caggacccca ttccgaaggt gaaccacccg ctcatcgacg
     6181 cgcaggacat cgctgcgctc aagggaaaga tc
//
LOCUS       DATDEU010000508         9125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_680729, whole genome shotgun sequence.
ACCESSION   DATDEU010000508 DATDEU010000000
VERSION     DATDEU010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680729"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1971
                     /locus_tag="VIV63_12170"
     CDS             <1..1971
                     /locus_tag="VIV63_12170"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HEY6125398.1"
                     /translation="VDRLRVVSNGGQNLRIDPDTGATTTDVALTSSGAPRAGINGAAY
                     SNAFAAACRTTLYYIDATTDQLLTSSDPNSGAVTVVGALGVDATDVGDFEIATAADGT
                     NTGYAVLVVGNVPTSYQIDLTTGVAATAGAVTLLDASEQVRDTAIAPPATAPTQLAGD
                     VYGLTETNKLVSFNAALPQKACTSATLSGQQASENIVGIDVRPADTALYALGSTGRVY
                     TVDTATAALTLKSTLAPQLGDVTAPYTALTGTAFGVDFNPGNDVLRVASDTGLNARID
                     VDDGVTTTDTPTNPVGPVVAEAAYSNSFVGAGTAFYYTIDAANDGLQVLGRPSGNAIN
                     GDLQTVGALGVGDVSTVAGFDIFGTNNQGLAALNVGGAATSDLFTINLLTGAATRINT
                     VGGGERLRGLAYARLPAATVFALTSENRLVSFKPLTPGTFDTDVAITGLQGSEAIVGF
                     DFRASNGLLYAVTDAGRVYTLDVATGAASGALTLSANPADITAPFTALAGTRFGVDFS
                     ALDNVLRIQSDTGQNLRVDVDAGTVITDVNLNPGTPQVVGTAFSNSYTGATGTTQYEL
                     DVAAGALVRQTSNATLTPVGSFNAGTTFTLEGGFDIAGGENGLPLAALQPTGAPQSTL
                     YRVNLGSGALTSIGLLGSSGTTVVRALAIRLQ"
     gene            2046..2732
                     /locus_tag="VIV63_12175"
     CDS             2046..2732
                     /locus_tag="VIV63_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125399.1"
                     /translation="MKWVVILLIVVVVGAVGAYWYEGYRAESTLLEQPVYRVLKKHEP
                     VVFEKLVAEYKIYQREETTREAFINFANSEINLAATQSLAHASQTSVLALMKDMLATA
                     RQLQKVPGDTCFRYWFPLVSGPPDVAKYIEPAAQAHTLELMGDVIRSAAEDPTPLPPA
                     DSVKDNLATVINGTYEQYGSDAQMIAHAEDPRVDRAKVCTITLSVYDRIMSLPPADSS
                     ALIRSMTQLR"
     gene            2736..3254
                     /locus_tag="VIV63_12180"
     CDS             2736..3254
                     /locus_tag="VIV63_12180"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEY6125400.1"
                     /translation="MPLSHADRILKVNHAGEHGAVNIYRGQRLVCFWRDDALKRELEE
                     FRAHEEKHRAIFAAELARRGVRRCRSYWACGLGGFLLGVSTGLCGRASIAAVTVAVER
                     VVLRHLEVQLRELDGVDAAAHAAVASILEDERAHHDRAAQEPRQGIFWPRVLKPTVSA
                     MTEFVIWTGMKL"
     gene            3465..4397
                     /locus_tag="VIV63_12185"
     CDS             3465..4397
                     /locus_tag="VIV63_12185"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6125401.1"
                     /translation="MRTPSLKAIKTFQIAAKHSSFAVAADELCITPSAVSHQIKTLET
                     QLGLPLFSRSARALALTDAGSRYLEQIDDLFMRLDSVTEQLRARYGRNSVRLHVPAYF
                     ASEMLLPRLSEFSKLHAGIDLRIETQSVHGRTHAAEADISVVVGSGPWNNALIAHPLF
                     EQTMVPACSPELFAERPVKRYEDLNAHTLLVHEGRRDDWDRWATAVGMKQLQPAQIVR
                     IDSMSAATRAAEKSIGIALLPAELSRRKFDSGRLIRVFDDELVTHEEYTLLVRTEDEN
                     RDDIRALCAWLMELAAPMNKTDARAESSRLLKQA"
     gene            4446..4766
                     /locus_tag="VIV63_12190"
     CDS             4446..4766
                     /locus_tag="VIV63_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125402.1"
                     /translation="MIRRTSKYLVMAAVISFASYANAGTAVVTPALAACSKALIETIA
                     KTEPLPAYKVKAPSAFVSELLDPNAFTVIARHSKTNELLAKASCKATSAGEIVSFRSI
                     PLKS"
     gene            4945..5562
                     /locus_tag="VIV63_12195"
     CDS             4945..5562
                     /locus_tag="VIV63_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6125403.1"
                     /translation="MTGSAEPSSISAGFPTLPPLAPQSAARWRRYLGILLMWISGWRI
                     AGNIPNVSKYVLIVAPHTSNWDFFHGFCAFLVLRLDNTWLAKHTVFFWPLGILARRFG
                     GMPIDRAKGGNVVRTCVSEFARRERISFTITPEGTRGKVKEWKLGFYYIATEAGVPIV
                     PVALNYSKKLVMIMAPFYPTGDAATDMPKIKGLYSAEMAKHPESF"
     gene            5573..6703
                     /locus_tag="VIV63_12200"
     CDS             5573..6703
                     /locus_tag="VIV63_12200"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6125404.1"
                     /translation="MTSLLVLSVSAGAGHVRAADALVKAAEAAHPPLAATHIDVLTLV
                     PQEFKKLYGEQYIRLVEKMPQLWSFLYSKSDRPSRDSLVGKLKRAAEKLNTRKLSAEI
                     ERLAPDVILCTHFLPAELLSRERGQDKKKGRKLPRLWVQVTDFDVHALWVHPNVDRYC
                     VANEEVAFRLADRGVPREKISITGIPVMPQFSAPLERAACARELGLSPDKFTVLMMAG
                     GAGVGSLDELAERLLRLPDDMQLVALAGRNAELLKRLQALAKRHPGKLFPLGFTTTVE
                     RVMTAADLVVTKPGGLSVSECLAKHRPMLLVSPIPGQEERNADYLLEAGAALKAVDGA
                     TLAFKLARLLADRPRLAAMSAAAARISRPQAGADVIALISSS"
     gene            6700..7293
                     /locus_tag="VIV63_12205"
     CDS             6700..7293
                     /locus_tag="VIV63_12205"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEY6125405.1"
                     /translation="MKTSPAFWLCALLAAPVSAQDAATQTPPAAVARPAEWAEPIALD
                     GVPNLHRITPALYRSEQPSALGMKNLEKLGIRTVINLRAFNSDDDEVEGTSLRTERTK
                     ILTWRIGDKHVVEVMNMLKKTENGPFLIHCQHGADRTGLMTAMYRILEQGWSPQDALQ
                     ELTDGGYGYHSLWTNIIEYVQSADVGKLKAAIAASAN"
     gene            complement(7290..8198)
                     /locus_tag="VIV63_12210"
     CDS             complement(7290..8198)
                     /locus_tag="VIV63_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY6125406.1"
                     /translation="MSRKYRVRHETTYKYAADVMHSHHLLHLVPRPAPYQQCLEHEIQ
                     IEPASHRRVNQLDAFGNPLIRIELAQPHRELTVISEMQIEVHTRPAVSPETTDAWEKV
                     LESFAYHGAWPSRDQLDAARFRHESPHVRVKQSFTDYSAVCFGEGRPILACAEALSTK
                     LHADIKYVPGVTTTSTSPTEVLETRRGVCQDFAHFMISCLRSRGLPARYVSGYLRTNA
                     TQGEGGEKLVGDAASHAWVSVWSPPYGWIEFDPTNGCFAGTDHIAVAWGRDFGDVSPL
                     RGVILGGADHRLSVTVRVEPEGDSPN"
     gene            complement(8223..>9125)
                     /locus_tag="VIV63_12215"
     CDS             complement(8223..>9125)
                     /locus_tag="VIV63_12215"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY6125407.1"
                     /translation="LVENLFWLGRYAVRCENTSRLLLRTLGARSDARVWAHAQKICRD
                     LGVVGKEGEVLDALRTRDSVGLQADVKHLAWCASQVRNRLSARYWRGVVGLQRQMQEA
                     LATRGSSREVYERVLLSLAALTGFSEEDMMHDEGWRVMRLGRRIERMQFVANILVRQL
                     ESSHATRPEAVEWLLDVCDSTPIYRARYLGIPRLSQMLNLLLYDDGHPMALAFHRRSV
                     DRDLDDLARSLGGERERGVPDVPLLPQGSAELLDAVGEEGDAARAQLAEELAGLSRRA
                     GELSDRVSRRYFALIESDAQALAT"
BASE COUNT         1675 a         3041 c         2873 g         1536 t
ORIGIN      
        1 gtggatcgtc tgcgcgttgt cagcaatggc ggacagaatc tgcgcatcga ccccgacacg
       61 ggcgcgacga cgaccgacgt cgccctgacg agcagcggcg ccccgcgcgc tggcatcaac
      121 ggcgcggcct acagcaacgc attcgcggcc gcctgccgca cgacgctgta ttacatcgat
      181 gcgaccaccg accagctcct gaccagcagc gatccgaatt cgggcgcggt cacggtggtc
      241 ggcgcgctgg gcgtcgatgc gaccgacgtg ggcgacttcg aaatcgcgac ggcagccgac
      301 ggcacgaaca ctggttacgc cgtgctggtg gtgggtaacg tgccgacttc ctaccagata
      361 gacctcacta ccggcgtcgc ggccacggcg ggcgcggtga cactgctcga tgcgagcgag
      421 caggttcgcg acaccgcgat cgcgccaccg gccactgcgc cgacgcagct ggcgggcgac
      481 gtttacggac tcaccgaaac caacaagctg gtgtcgttca atgcggcgct cccgcaaaag
      541 gcatgcacga gcgcgacgtt gagcggtcaa caggcgagcg agaacatcgt cggcatcgac
      601 gtgcgtcccg cggatacggc cctgtacgcg ctcggcagca cgggtcgcgt ctacacggtg
      661 gacacggcga cggccgcgct gacgctcaag tcgacgctgg caccacaact cggcgacgtc
      721 accgcgccgt acacggccct cacgggcacc gcctttggcg tggacttcaa tccgggcaac
      781 gacgtgctgc gcgtcgccag tgacaccggc ctcaacgcac gcatcgacgt ggacgatggc
      841 gtgacgacca ccgacacgcc gaccaatccg gtgggcccgg tggtcgccga ggcggcgtac
      901 agcaacagct tcgtcggcgc cggcacggcg ttctactaca ccatcgacgc ggcaaacgat
      961 ggcctgcagg tgctgggacg cccctcgggc aatgccatca acggcgatct gcagaccgtg
     1021 ggcgctctcg gcgtgggtga cgtctccacc gtcgcgggct tcgacatctt tggcaccaac
     1081 aaccagggac tggcggctct caacgtgggc ggcgcggcca cgtccgatct gttcaccatc
     1141 aatctcctca cgggcgcggc gacgcgtatc aacaccgtgg gcggtggtga acggttgcgc
     1201 ggcctcgcct acgcacgact gcccgccgcc acggtattcg cgctcacgtc ggagaatcgt
     1261 ctggtgagct tcaagccgct gacgcccggc acgttcgaca ccgatgtggc gatcacgggc
     1321 ctgcaaggca gtgaagcgat cgtcggcttc gacttccgtg cctctaacgg cttgttgtac
     1381 gccgtcacgg atgcgggccg ggtatacacg ctcgatgtgg cgaccggtgc cgcgtccggt
     1441 gcgctgacct tgagcgccaa cccggccgac atcacggcgc cgttcacggc gctcgcgggt
     1501 acacgattcg gggtggactt cagtgcgctc gacaacgtgc tgcgcatcca gagcgacacg
     1561 gggcagaacc tgcgcgtgga tgtggacgcc ggcacggtca tcaccgacgt gaacctgaat
     1621 ccggggactc ctcaagtggt ggggaccgcg ttctccaaca gctacaccgg cgccaccggc
     1681 accacgcagt acgagctcga cgtggcggcg ggcgcactgg tgcggcagac ctccaatgcc
     1741 acgctgaccc cggtgggctc gttcaatgca ggcaccacgt tcacgctcga aggcgggttc
     1801 gacatcgccg gcggtgagaa cggcctgccg ctcgcggcgc tgcaaccgac cggcgctccc
     1861 caatcgacgc tgtaccgggt gaatctcggc agcggcgctc tgacctcgat cggattgtta
     1921 gggtcgagcg gcacgacggt agtcagggcg ctggccatcc gcctgcaatg agtccgtgac
     1981 gacagggcgg cagccgtccg gctgccgccc atgggcggag ccgccgaaca gggtattctc
     2041 cagccatgaa atgggtcgtc atcctgctga tcgtcgtagt cgttggcgcc gtcggagcct
     2101 attggtacga gggttaccgc gccgaatcga cgctgctcga gcagcctgtc taccgggtac
     2161 tcaagaaaca cgagccggtg gtattcgaaa aactggtggc ggaatacaag atctaccagc
     2221 gcgaagagac cacgcgcgag gcgttcatca acttcgccaa ctccgaaatc aacctcgccg
     2281 cgacgcagag cctggcgcac gcctcgcaga cttccgtgct ggcgctcatg aaagacatgc
     2341 tggcgaccgc gcgtcagttg cagaaggtgc ccggcgatac ctgcttccgt tactggttcc
     2401 cgctggtgtc ggggccaccg gatgtcgcga agtacatcga accggccgcg caggcgcaca
     2461 cgctcgaact catgggtgac gtcatccgca gcgccgccga agatccgacg ccgttgcctc
     2521 cagcggactc ggtcaaggac aacctcgcga ccgtcatcaa cggaacctac gaacaatacg
     2581 gctccgacgc gcagatgatc gcgcacgccg aagatccgcg cgtggaccgc gccaaggtgt
     2641 gcaccatcac gctgtcggtc tacgaccgca tcatgagcct gccgcccgcg gacagcagcg
     2701 cgctgatccg gtcgatgacg cagctgcgct gaccggtgcc gctgtcccac gccgatcgca
     2761 tcctcaaggt gaaccacgcc ggtgaacacg gcgcggtgaa catatatcgc gggcaacgcc
     2821 tcgtgtgttt ctggcgcgac gacgcgctca agcgcgagct cgaggaattc cgtgcgcacg
     2881 aggaaaaaca ccgcgcgatc ttcgcggccg aactcgcgcg gcgcggtgtg cggcgctgcc
     2941 ggagctactg ggcgtgcggc ctcggcgggt tcctgctcgg cgtcagcacc ggcctctgcg
     3001 gccgcgcgtc cattgccgcg gtgaccgtgg ccgtggaacg cgtggtcttg cggcacctgg
     3061 aggtgcagct tcgcgagctg gacggcgtcg acgcggccgc gcatgccgcc gtagcgtcca
     3121 tcctcgaaga cgaacgcgcc catcacgacc gcgccgcgca ggaaccgcgc cagggaatct
     3181 tctggccccg ggttctcaag cccaccgtca gcgccatgac ggagttcgtc atctggacgg
     3241 gcatgaagct ctagcgatat gtaaacgccc gcctgtcaca aatgcttcac ccgtacccgg
     3301 tgcaaccgcg ggatttgcgc gccacggacg aacattgctg acaccccgtc aactgggcag
     3361 gccgaataag aacatgcact tacgtcattt ccaccggtgg cacgcgcctt gctttactcc
     3421 tggcatcgca gctttttcgc tgagttctta ggtgatccaa tggaatgcgt actccgtcac
     3481 tgaaggccat caagaccttt cagatcgccg ccaaacactc gagcttcgcc gtcgcggccg
     3541 atgagctttg catcacgccg tcggcggtaa gtcaccagat caagacgctg gaaacgcagc
     3601 tgggcctgcc gctgttttca cgtagtgctc gggcattggc gctcaccgat gccgggtctc
     3661 gctatctcga acagatagac gacctgttca tgcgactgga ttccgttacc gaacagcttc
     3721 gcgctcgtta cggacgcaac agcgtgcgcc tgcacgtgcc ggcgtatttc gcgagcgaga
     3781 tgctgctgcc gcgcctgtcg gagttctcga agctgcacgc cggcatcgac ctgcgcatcg
     3841 agacgcagag cgtgcacggc cgcacgcacg ccgcggaggc cgacatatcg gtggtagttg
     3901 gatctggacc gtggaacaac gcgctcatcg cgcacccgct gttcgaacag accatggtgc
     3961 cggcgtgttc gccggaactc ttcgccgagc ggccggtgaa gcgttatgaa gatctcaacg
     4021 cgcacacctt gttagtccac gaaggacgcc gagacgactg ggaccgctgg gccaccgccg
     4081 tcggcatgaa acagctgcag cccgcgcaga tcgtgcgcat cgactccatg tccgcggcga
     4141 cgcgcgccgc ggaaaagagc atcggcatcg cgttgctgcc cgccgaactc tcgcgccgca
     4201 agttcgacag cggccggctg atacgcgtgt tcgatgacga actcgttaca catgaggagt
     4261 acacgttatt ggttcgaacg gaagacgaaa accgtgacga cattcgcgct ttgtgcgcgt
     4321 ggctcatgga actcgccgca ccgatgaaca aaactgatgc tcgcgccgaa tcttctcgtt
     4381 tgttgaagca ggcctgagcc agtagtttga cactcgtggt tatcaaacat cttcaggagt
     4441 gcaacatgat tcgtcgaacc tctaagtacc tcgtcatggc cgctgtcatc agcttcgcgt
     4501 cctacgcgaa tgccggcacc gccgtcgtca cgcctgcgct cgccgcgtgc agcaaggccc
     4561 tcatcgagac catcgccaag acggagccgc tgccggctta caaggtcaag gcgcccagcg
     4621 cattcgtctc ggaattgctc gacccgaacg ccttcacggt gatcgcgcgt cattccaaga
     4681 ccaacgagct gctggccaag gcgtcgtgca aggcgacttc ggccggtgaa atcgtttcgt
     4741 tccgttcaat tccgctgaag agctaataga actgccgagg agctaactat cgaccgcact
     4801 ctcgaacacg gcgaggcgcg ggcaccggtt cccccatccc ccctctagcg gggaaacggt
     4861 gtccgcctct cgaccgcctt accttagaga gttgcggatt tcggcaaagc gcgtacgggg
     4921 ccatttcggc taccatcgcg cggcatgaca ggctccgccg aaccttcctc gatttccgcc
     4981 ggtttcccca cccttccccc gctggctccg caaagcgcgg cgcgatggcg gcggtatctc
     5041 ggcatcctgc tcatgtggat ttccggctgg cgcatcgccg gaaacatccc caacgtctcc
     5101 aagtacgtgc tgatcgtggc cccacacacg tccaactggg actttttcca cggcttctgc
     5161 gcgttcctgg tcctgcggct cgacaacacc tggcttgcca agcacacggt cttcttctgg
     5221 ccgctcggca tcctggcgcg caggttcggc ggcatgccca tcgaccgcgc caagggcggc
     5281 aacgtggtgc gcacctgcgt ttcggaattc gcgcggcgcg aacgcatctc tttcaccatc
     5341 acccccgagg gaacccgcgg caaggtgaag gaatggaagc tcggcttcta ttacatagcg
     5401 accgaagcgg gcgtccccat cgtcccggtg gcgctgaact attcgaagaa gctggtcatg
     5461 atcatggcgc cgttctaccc caccggtgac gcagccaccg acatgccgaa gatcaagggt
     5521 ctttattcgg ccgagatggc gaagcacccc gaaagtttct gatcagcgaa acatgacctc
     5581 actcctggtt ctttcggttt cggcaggcgc cggccacgtg cgcgccgcgg acgcgctcgt
     5641 caaggcagcc gaagcggcgc atccgccgct cgcggccacg cacatcgatg tgttgacgct
     5701 ggtgccgcag gagttcaaga aactctacgg cgagcaatac atccggctgg tggagaagat
     5761 gccgcagctc tggtctttcc tctattcgaa gtccgaccgg ccgagccgcg attcgctggt
     5821 gggcaaactc aagcgcgccg ccgagaaact caacacgcgc aagttgagcg ccgagatcga
     5881 gcggctcgcg ccggatgtca ttctctgcac gcatttcctc ccggccgaat tactgtcgcg
     5941 cgagcgtggc caggacaaga agaagggcag aaaactccca cggctgtggg tgcaggtgac
     6001 ggacttcgac gtgcatgcat tgtgggtgca cccgaatgtg gatcggtatt gcgtcgccaa
     6061 cgaagaagtc gcgttcaggc tcgccgaccg cggagtgccg cgggagaaga tttccatcac
     6121 cggcattccg gtgatgccgc agttcagcgc gccgctcgaa cgcgcggcct gcgcacgtga
     6181 gctcgggctg tcgcctgaca agttcacggt gctcatgatg gccggcggcg cgggcgtcgg
     6241 cagtctcgac gaactcgcgg agcggttgct gcggctgccc gatgacatgc agcttgtcgc
     6301 gcttgcgggc cgaaatgcgg aattgctgaa acgcctgcag gcgctcgcga aacggcatcc
     6361 cggaaagctc tttccgctcg gattcacgac cacggtcgaa cgcgtgatga ccgccgccga
     6421 tctcgtcgtg acgaagcccg gcggactgtc cgtgtcggag tgcctggcga aacaccggcc
     6481 gatgctgctc gtgtcaccga tcccgggcca ggaagagcgc aacgcggact atctactgga
     6541 agcgggcgcg gcactcaagg ccgtcgacgg cgctacgctc gccttcaagc tcgcccgcct
     6601 gctcgccgat cgcccgcggc tggcggccat gagcgcggcg gcggcccgca tcagtcgtcc
     6661 gcaggcgggc gccgatgtca ttgcgctaat atcttcgtca tgaaaacttc tcccgccttc
     6721 tggttgtgcg cgctgctcgc cgcgccggtg tcggcgcagg acgccgccac acaaaccccg
     6781 cccgccgcgg tggcgcggcc cgccgaatgg gccgaaccca ttgccctcga cggcgtgccc
     6841 aacctccatc gcatcacgcc cgcgctctat cgcagcgagc agccgagcgc gctcggcatg
     6901 aaaaacctgg aaaagctggg catccgtacg gtcatcaacc tgcgcgcctt caacagcgac
     6961 gacgatgaag tcgagggcac ctcgctgcgc accgaacgta ccaagatcct cacctggcga
     7021 atcggcgaca aacacgtcgt cgaagtcatg aacatgttga agaagaccga gaatggcccg
     7081 ttcctcatcc attgccagca tggcgcggat cgcaccggac tgatgaccgc catgtaccgc
     7141 atcctcgagc agggctggag cccccaggac gcgctgcagg aactgacgga cggcggctac
     7201 ggctaccact ccctgtggac caacatcatc gagtacgtgc aatcggcgga cgtcggaaag
     7261 ctgaaagccg cgatcgcagc gtccgcgaac tagttcgggg aatcgccttc cggctcgacg
     7321 cgcaccgtca ccgacaatcg atgatccgct ccaccgagga tcacgccgcg aagcggtgat
     7381 acgtcaccga aatcgcggcc ccaggccacg gcaatatgat ccgtgcctgc gaagcagccg
     7441 ttggtggggt cgaactcgat ccagccgtaa ggcggactcc acaccgacac ccaggcgtgt
     7501 gatgccgcat cacccacgag cttttcgccg ccttcaccct gcgtggcgtt ggtacgcagg
     7561 tacccgctga cgtagcgcgc cggcagtccg cgtgaacgca gacaggagat catgaaatgc
     7621 gcgaagtcct ggcagacgcc gcgacgcgtt tcgagcacct cggtgggaga cgtgctcgtg
     7681 gtggtcacac cgggtacgta cttgatgtcc gcgtgtagtt tggtgctcaa cgcttcggcg
     7741 caggccagga tgggccgtcc ttcgccgaaa cacaccgccg agtagtcggt gaacgattgc
     7801 ttcacgcgca catgcggcga ttcatgacga aagcgcgcgg catcgagctg gtcgcgcgac
     7861 ggccaggcgc catgatacgc gaacgactcg agcactttct cccacgcatc cgtggtttcg
     7921 ggcgagacgg cgggccgggt gtgcacttcg atctgcatct ccgaaatcac cgtgagctcg
     7981 cgatgcggct gcgcgagttc gatgcggatc agcggattgc cgaaagcgtc gagctggttc
     8041 acgcggcgat ggctcgccgg ctctatctgg atttcatgct ccaggcactg ctggtaaggc
     8101 gcggggcgcg gtaccaggtg cagcaggtga tgcgagtgca tcacgtcggc ggcgtacttg
     8161 taagtcgtct cgtggcggac gcggtacttt ctgctcatgg aatggggctc atggaatggg
     8221 gctcatgtgg cgagcgcctg cgcgtcggat tcgatgagcg cgaaatagcg gcgcgatacg
     8281 cggtcggaaa gctcaccggc tcgcctcgac agaccggcga gttcttcggc gagctgggcg
     8341 cgcgccgcgt caccctcctc cccgacggca tccagcaatt cggccgagcc ttgcggcagc
     8401 agcggaacat cgggcacgcc gcgctcgcgc tcgccaccca ggctgcgcgc gagatcgtcg
     8461 aggtcacgat cgacggagcg gcggtggaag gccagcgcca tcgggtggcc gtcgtcgtac
     8521 agcagcaggt tcagcatctg cgacaggcgc ggaatgccga gatagcgcgc gcgatagata
     8581 ggcgtgctgt cgcagacgtc gagcagccac tcgacggcct cgggccgcgt ggcatggctg
     8641 ctctcgagct ggcgcacgag gatgttcgcg acgaactgca tgcgctcgat gcgccggccg
     8701 aggcgcatca cgcgccagcc ttcgtcgtgc atcatgtcct cttcggagaa accggtgagc
     8761 gcggcgagcg acagcagcac gcgctcgtag acctcgcggc tgctgccgcg cgtcgccagc
     8821 gcttcctgca tctgccgctg cagcccgacg acgccgcgcc agtagcgcgc cgacagccgg
     8881 ttgcgcacct gcgatgcgca ccacgcgaga tgtttgacgt cggcctgcag gcccaccgag
     8941 tcgcgcgttc gcagcgcgtc gaggacttcg ccctctttgc ccaccacgcc caggtcgcgg
     9001 cagatcttct gggcatgcgc ccacacccgg gcatcactgc gcgcgccgag cgtgcgcaac
     9061 agcagccgcg acgtgttttc acagcgcacg gcataacgac ccagccagaa gagattctcg
     9121 acaag
//
LOCUS       DATDEU010000509         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_242184, whole genome shotgun sequence.
ACCESSION   DATDEU010000509 DATDEU010000000
VERSION     DATDEU010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242184"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VIV63_12220"
     CDS             complement(<1..293)
                     /locus_tag="VIV63_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125408.1"
                     /translation="MYAIKVEIEDPRAKTFTFNAQKTMYGGKLIAEGDVIYIFASENE
                     GGAGLIAKGVVTRASATPRKPGIERQTPRVSITVKRTALAKKPMGRTEIKPFS"
     gene            complement(308..1171)
                     /locus_tag="VIV63_12225"
     CDS             complement(308..1171)
                     /locus_tag="VIV63_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY6125409.1"
                     /translation="MHRRAFLFRAGGLIIAVAGAGVAHAARSAPDRVGLGTVIFRKRF
                     DQKKLKLADVPAYYRERFGIRNLEFWSHHFESLEKPYLKELRSRVKAAGAKLINVQVD
                     ATYDLASTDEVKRQQSLETVRQWIDAAALSGSQAVRINPGRPDGSVEKSIASMKEVNR
                     YCLSKRLPLLTENHFGLEMNPDVHLRIREAAGPKNIYTLPDFGNYPVETMWESLAKIL
                     PYAYVVSAKAVDFNEKGEHISYDFDRCIQMCERAGFKGIYLVEQWSRKDQDLDDEKIA
                     DWMIQRVRANI"
     gene            1248..>3161
                     /locus_tag="VIV63_12230"
     CDS             1248..>3161
                     /locus_tag="VIV63_12230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6125410.1"
                     /translation="MRLLLVTPPMIQLNTPYPATAYLTGFLRLHAKDLGLEVAQADAS
                     ISLFLRLFSTPLVTRMAEELRRRAKSAGKRAVVPPSIAHFIANAERYAETVTPAIRFL
                     QRRDPSMAFRIVGRAFLPEGPRFEQVHGEQILSAFGALGTTEQARYLASLFVDDLADV
                     WRLGIDPRFELARYGEKLAASSATFDPLHAALTGEPTLVDETLDELTRELLAEQRPDI
                     VAITAPFPGNVYGAFRMARSIRAANPKATLILGGGWVNTELRSLRDPRVFDFFDYVTL
                     DDGERPLMSLLQRLSGRETKLVRTYVRKGDAVVLETDPMQHDIPQKDTGIPTYDGLPL
                     DSYVSVMEMLNPMHRFWSDGRWNKITLAHGCYWKKCSFCDVSLDYIGRYDEPSTDLIM
                     KRIKALIAETGETGFHLVDEAAPPSAMRSLAKRLIAEKLSITWWGNIRFEKTFTPELC
                     RQLAESGCVAVSGGLEVASDRLLQLMKKGVTVEQVARVTRAFTDAGIMVHAYLMYGFP
                     TETEQDTVDALERVRQLFAAGCIQSAYWHRFSATAHSPIGLNPDAYGIELLPPPDITF
                     AHNDVNFHDPTGTDHDYLGSGLRKALYNYMHGIGLESDVREWFETPVRRVNGRKHGRR
                     SAAASVPRTTVPPN"
BASE COUNT          563 a         1030 c          952 g          616 t
ORIGIN      
        1 tgggagaaag gcttgatctc ggtacgaccc atcggcttct tcgccagtgc ggtccgcttg
       61 acggtgatgc tgacgcgcgg agtctgcctt tcgatgccgg gctttcgggg agtggcgctg
      121 gcccgtgtga ccacgccctt cgcgatgagt ccggcgccgc cctcgttctc actcgcgaag
      181 atgtagatga catcgccttc ggcgatgagc ttgccgccat acatggtctt ctgcgcgttg
      241 aacgtaaacg tctttgcgcg ggggtcttcg atctcgactt tgatggcgta cacggcgatc
      301 gtggcgctca gatattcgcg cgcacgcgct ggatcatcca gtcggcgatt ttttcgtcgt
      361 cgagatcctg atccttgcgg ctccattgct cgaccagata gatgcctttg aaacccgcgc
      421 gttcacacat ctgtatgcac cgatcgaaat cgtaggagat atgttctccc ttctcgttga
      481 agtccacggc tttcgccgag accacgtagg catacgggag aatcttcgcg agcgactccc
      541 acatcgtctc gaccgggtaa ttcccgaagt ccggaagcgt gtagatgttc ttgggtcccg
      601 ccgcttcgcg aattcgcaga tggacgtccg ggttcatctc caggccaaag tggttttcgg
      661 tgagcagcgg caggcgtttc gacaggcagt atcgattcac ctctttcatc gacgcgatgc
      721 tcttctcgac cgagccgtcg ggccttcccg ggttaatccg cactgcctgg ctgcctgaca
      781 acgccgccgc gtcgatccac tggcgcaccg tttccagact ctgctgacgc ttgacctcgt
      841 ccgtgctggc gaggtcatag gtggcgtcca cctggacgtt gatgagcttc gcgccggcgg
      901 ctttcacgcg cgaccgcagt tctttcagat agggtttctc gagcgactcg aaatggtggc
      961 tccagaactc gagattgcgg atgccgaagc gctcgcggta atacgcggga acatcggcca
     1021 gcttcagttt cttctggtcg aagcgcttcc ggaaaatcac cgtgcccaga cccacgcggt
     1081 cgggggcgga tcgcgcggcg tgagcgacgc ccgctccggc gaccgcaata atcaggccgc
     1141 cggcgcggaa cagaaatgct cgtcgatgca tgggtggccc tgcgtacacg tcaggcggcg
     1201 attgtagcta tgtcaccctg catccgcgtg cgatacgctg cgcgcccatg cgcctcctgc
     1261 tcgtcacgcc cccgatgatt cagctcaata cgccctaccc ggcgacggcg tacctgacgg
     1321 gattcctgcg cttgcatgcg aaggatctcg ggctcgaggt cgcacaggcg gacgcttcga
     1381 tatcgctgtt cctgaggctt ttttcgacgc cgctcgtgac gcgcatggca gaagaactgc
     1441 ggcggcgcgc gaagagcgcg ggcaagcgcg ccgtagtgcc accctctatc gcccacttca
     1501 tcgccaacgc cgagcgatac gcggaaaccg tgacaccggc gatccgcttc ctgcaacgcc
     1561 gcgatcccag catggcgttc cgcatcgtcg ggcgtgcgtt cctgcccgaa gggccgcgtt
     1621 tcgagcaggt gcatggcgag cagatcctgt cggcattcgg cgctctgggc acgaccgagc
     1681 aggcacgtta tctggccagt ttgtttgtcg acgatcttgc cgacgtctgg cgcctcggca
     1741 tcgatccgcg ctttgagctc gcgcggtacg gtgaaaagct cgccgcgagc tccgctactt
     1801 tcgacccgct gcatgccgcg ctgacgggcg aacccacact cgtcgacgaa acactcgacg
     1861 agctcacgcg cgaactactc gcggagcaac ggccggacat cgttgccatc accgcgccgt
     1921 ttcccggcaa cgtctacggc gcgttccgca tggcgcgctc gattcgcgcc gcgaatccca
     1981 aggccacgtt gatcctgggc ggcggctggg tgaacaccga gttgcgctcg cttcgcgatc
     2041 cgcgcgtgtt cgacttcttc gattacgtca ctctcgacga cggcgagcgg ccgctgatga
     2101 gtctgctgca gcggctgagc ggccgcgaaa cgaaactggt gcgcacctac gtgcgcaagg
     2161 gcgatgcggt cgtgctcgaa accgacccga tgcaacacga catcccgcag aaagacacgg
     2221 gcatcccgac gtacgacgga cttccgctcg acagctacgt ttccgtcatg gaaatgctca
     2281 acccgatgca tcgcttctgg tcggacggcc gctggaacaa gatcacgctg gcgcacggct
     2341 gctactggaa gaagtgctcg ttctgcgatg tatcactcga ctacatcggc cgttacgacg
     2401 agccgagcac cgacctcatc atgaagcgca tcaaggcgct gatcgcggaa acgggcgaaa
     2461 cgggctttca tctcgtcgat gaagccgcgc caccctccgc catgcgttcg ctcgcgaaac
     2521 gcctgatcgc agaaaagctg agcatcacgt ggtggggcaa catccgcttc gaaaaaacct
     2581 tcacgccgga actctgccgc cagctcgccg agtccggctg cgtggccgtg agcggtggcc
     2641 tcgaagtggc ctcggaccgc ctgctgcaac tcatgaagaa aggcgtcacc gtcgaacagg
     2701 ttgcgcgcgt gacgcgcgcc ttcaccgatg ccggcatcat ggtgcacgcc tatctcatgt
     2761 acggatttcc cacagagacc gagcaggaca cggtggatgc gctcgaacgc gtgcgccagt
     2821 tgttcgccgc gggctgcatc cagtcggcct actggcatcg gttctccgcg accgcgcatt
     2881 cgccgatcgg cctgaaccct gacgcctacg gcatcgagct gctgccgccg ccggacatca
     2941 cgtttgcgca caacgacgtg aattttcacg accccaccgg tacggatcac gactacctgg
     3001 gcagtgggct gcgcaaggcg ctctataact acatgcacgg catcggactc gaatccgacg
     3061 tgcgcgagtg gttcgaaacg ccggttcgcc gcgtcaacgg tcggaaacat ggccgacgca
     3121 gcgctgcggc gtcggtaccg cgcaccacgg tgccgccaaa c
//
LOCUS       DATDEU010000510         4725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1410688, whole genome shotgun sequence.
ACCESSION   DATDEU010000510 DATDEU010000000
VERSION     DATDEU010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410688"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..2273
                     /locus_tag="VIV63_12235"
     CDS             132..2273
                     /locus_tag="VIV63_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125411.1"
                     /translation="MEFVNFPTVALGAALICLIGVTIYAITQRGSLRSRDEQLVVARA
                     QAYAMLELSQAGVLFLDRDHKLMGEASAAAPELLGHTCGAGTAFVQVIADLVDVHVRR
                     QTVAFLESMWKAEASAVVDATQNPLDKVHSGARHLAIRFARLVVDGRVHHVIVSIERI
                     SAPRVMPLTIEVPVLDEDTFAKRLAGETGTRPALNVAAMAAMPEDVPVVAPAPPAVSI
                     VAPLSQADIPTISPAPEAPASAAAPAPMSTGEFELGPLDATEDSIPSVPALNDAVMSA
                     MATPSGSADKTQKIAALPASLLPLAPASNAVTATMRGLEDEQHLATTITSPDAAPAAK
                     AAAKPAAPFDPSDIIDPPDARLSEVLKEIMHIDAARLETFLAEAREKAGQLRAIMKLP
                     AREPQAFREKLVLILELIRGIHARAERLPLPSVCERSERFSAALNALRDKQTLSGNDF
                     LPLAVKLDDLLSHLAIQGEVVMRLRDWRTQNGDSGSPSIDATQRSATATGTTIRQPQL
                     RGAAPSATGITKAARLSDLSQESLEEMSVFLADMYSKRVSLVVVGLEDVPSHYRRSIE
                     KILGQLIHNAIRHGVENPSDRLNHDKPEIGTVAVQFSRLGPDGYQLSVQDDGRGLDHD
                     KIRAEAVRQGVLTPDVAAKIDPRKLASLIFRPGFTTVGDDGARGIGMDVVRDLVTRAG
                     GRVGIATKPGEYTRFRITLPHEKKASDAAVA"
     gene            complement(2294..2491)
                     /locus_tag="VIV63_12240"
     CDS             complement(2294..2491)
                     /locus_tag="VIV63_12240"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEY6125412.1"
                     /translation="MPSSVIRSYHYDPLQRRLELQFVSGRRYRYHDVPEETWRGMRQA
                     FSKGEFFNANIRDHFRYTRES"
     gene            complement(2544..3848)
                     /locus_tag="VIV63_12245"
     CDS             complement(2544..3848)
                     /locus_tag="VIV63_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125413.1"
                     /translation="MTQQLDVFLARFVRIEPGEKRAVFTAFALMFCVMGGYFAVRPIR
                     ETVGTILGEAITSDTWIWTAVFAIAIIPVYGYLVARVSRATLVPAIYGSVAIALAATG
                     MVFRADPENMVVGRVFYVGISVINLFLISVFWSFLLELFRSEQTKRLFGFIAAGGTTG
                     ALVGPLITDRIVNQVGISGVLFFGAAMFFIAIFCQRALLAEMRAHADLAAVAAGGAAG
                     EAPRVNRGVGGNPFAGVAIVLKSPYLLGIALFVIMISTANTILYFEQLRLVKDMFDNP
                     EERTQMFARIDFIVQTLTVISQLLITGRLAHKLGVTALLVILPLLIMGGFLLLASMGT
                     FLILAGVMILRRWGEYAFIRPGREMLWSRLDTETKYKAKNFIDVTVYRFSDAVVAQVQ
                     DALKAGGMNPAQGALLGAGVCAVWAVNGWWLGRKFDKGKQES"
     gene            complement(3894..>4725)
                     /locus_tag="VIV63_12250"
     CDS             complement(3894..>4725)
                     /locus_tag="VIV63_12250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6125414.1"
                     /translation="ILQDPYKFESADLVLIESTYGNRQHRGREDTERELGGILEQAAR
                     DGGNVIVPAFAIGRSQELLYLLAKHYGEWHLTRWKIFLDSPMAIAASRVYWSNPDRFD
                     EETTRLRESFRGMPPLPNLVLSESADDSRAINHLRSGALIIAGSGMANGGRVLHHLKH
                     NLERAECHVVIVGFQAPGTLGRQLVDRRPEVFIHGQRVRCLAQIHTLGGLSAHGDQDD
                     LLRWYDSFAARPPVYLVHGEVPSAEALAEKLRGRGASATVSRPGLTIDLANLPALSRD
                     "
BASE COUNT          848 a         1613 c         1493 g          771 t
ORIGIN      
        1 aacagcgcat cgccacctgc gacgagctgt tggtcgtgct cacggaaatg tcgcacgcca
       61 tgagcgatcg cgtcatgatc gatcccgaga ctcttgtctc ggccggcgcc gccccttaag
      121 gaggaggacg catggaattc gtgaacttcc ccacggtggc gctcggcgcg gcgctcatct
      181 gcctgattgg cgtcactatc tacgcgatca cccagcgcgg cagcctccgt tcgcgtgacg
      241 aacaactcgt ggtcgcgcgc gcgcaggcct acgccatgct cgagctgtcc caggctggcg
      301 tgttgttcct cgatcgcgat cacaagctca tgggcgaagc gtcggccgcg gccccggagt
      361 tgctgggcca cacctgcggc gcgggcaccg cgttcgtcca ggtcatcgcc gatctcgtcg
      421 acgtgcacgt gcggcgccag acggtggcgt tcctcgaatc catgtggaag gcggaagcca
      481 gcgcggtggt cgacgccacg cagaatccgc tggacaaggt gcactcgggc gctcgccacc
      541 tggcgattcg ttttgcgcgc ctcgtggtcg acggccgcgt tcatcacgtc atcgtttcca
      601 tcgagcgcat ctccgcgccg cgcgtcatgc cgctcacgat cgaagtgccc gtcctcgacg
      661 aagacacatt cgcgaagcgc ctggccggtg aaaccggcac gcggccggca ctcaacgtgg
      721 cggccatggc cgcgatgcct gaagacgttc ccgtcgtagc gccggcgccg cccgcggttt
      781 caatcgtcgc gcccctctcg caggctgaca tcccgacgat ttcccctgcc cccgaagcgc
      841 ccgccagcgc ggccgcgcca gctcccatgt ccaccggcga gttcgagctg ggaccgctcg
      901 acgccaccga agactccatc ccgtcagtgc ccgcactcaa cgatgcggtc atgagcgcca
      961 tggccacccc ttcgggcagc gccgacaaga cgcagaagat cgcggcgctg ccggcctcgc
     1021 tgctgccgct cgcaccggcg tccaacgccg tcaccgcgac gatgcgcggg ctcgaagacg
     1081 agcagcatct cgcgaccacg atcacttcgc cggatgcggc accggccgcg aaggccgccg
     1141 cgaaacccgc cgcgccgttc gatccgtcgg acatcatcga tccgccggat gcgcgtctca
     1201 gcgaagtgct caaggagatc atgcacatcg atgccgcgcg gctcgagacc ttcctcgccg
     1261 aagcacgcga gaaggccggc cagctccgcg ccatcatgaa actgcccgcg cgtgaaccgc
     1321 aggcgttccg tgaaaagctc gtgttgatcc tcgagctgat tcgcggaatc cacgcccgcg
     1381 ccgaacgcct gccgctgccg tcggtctgtg aacggtccga gcgattctcg gccgcgctca
     1441 acgcgcttcg cgacaagcag acgctgtccg gcaacgactt cctgccgctc gcggtgaagc
     1501 tcgacgatct gctgagccat ctcgccatcc agggcgaagt ggtgatgcgc ctgcgcgatt
     1561 ggcgtacgca gaacggcgac tccggttcgc cgtccatcga cgccacgcag cgttcggcca
     1621 ccgccacggg gaccaccatt cgccagccgc agctacgcgg cgccgctcct tcggccacgg
     1681 ggatcaccaa ggccgcgcgg ctctcggatc tctcgcagga aagcctcgag gagatgtccg
     1741 tgttcctcgc cgacatgtac tccaagcgcg tgtcgctcgt ggtcgtcggg ctcgaagatg
     1801 taccgagtca ctaccggcgc tccatcgaaa agattctcgg ccagctcatc cacaacgcga
     1861 tccggcacgg cgtcgaaaac ccgtccgatc gcctgaacca cgacaagccg gagatcggca
     1921 ccgtcgccgt tcagttctcg cgtctcggcc ccgatggtta tcagctcagc gtgcaggacg
     1981 acggccgcgg tctcgatcac gacaagatcc gcgccgaggc cgtgcgccag ggcgtgctca
     2041 ctcccgacgt cgccgccaag atcgacccgc gcaagctcgc cagcctcatc ttccgccccg
     2101 gattcaccac ggttggcgac gatggcgcgc gcgggatcgg catggacgtc gtgcgcgatc
     2161 tcgtcaccag ggccggcggt cgtgtcggca tcgcgacgaa gcccggtgag tacacccgct
     2221 tccgcatcac cctgccgcac gagaaaaagg cttccgacgc cgccgtcgcg taaaagggga
     2281 cagtcccctt tttctagctc tcccgcgtgt agcggaagtg gtcgcggatg ttggcgttga
     2341 agaactcacc cttcgaaaag gcctgacgca tgccgcgcca ggtttcctcc ggcacatcgt
     2401 gataccgata gcgccggccg gaaacgaact gcagctccag gcggcgctgc agcgggtcgt
     2461 agtggtacga acggatgacg ctcgaaggca tgagccgcag agttgagtcc gcggcctgaa
     2521 gttcccgggc tgccggacgc ttcctacgac tcctgcttgc ccttgtcgaa cttgcgcccg
     2581 agccaccagc cgttgaccgc ccacaccgcg cacacgcctg cgcctaacaa agcgccctgc
     2641 gccggattca tgccacccgc cttgagcgcg tcctgcacct gggccaccac ggcatcggag
     2701 aaccggtaca cggtgacgtc gatgaagttc ttggccttgt acttcgtttc cgtgtcgagc
     2761 cggctccaca acatctcgcg acccggacgg atgaacgcgt attcgcccca gcggcgcagg
     2821 atcatgacgc cggccaggat caggaacgtg cccatgctcg ccagcagcag gaagccgccc
     2881 atgatgagca gcggcaatat caccagcagc gcggtgacac cgagcttgtg cgccaggcgt
     2941 ccggtgatga gcaactgcga aatgacggtc agcgtctgca cgatgaaatc gatgcgcgcg
     3001 aacatctgtg tgcgctcttc gggattgtcg aacatgtcct tcacgagccg gagctgctcg
     3061 aagtagagga tggtgttcgc cgtggagatc atgatgacga acagcgcgat gcccagaaga
     3121 taaggtgact tgagcacgat ggcgacgccg gcgaacggat tgccgcccac gccgcgattc
     3181 acgcgcggcg cctcgcccgc cgccccaccg gcagcgaccg cggccagatc agcgtgcgcg
     3241 cgcatctcgg ccagcaatgc ccgctgacag aagatggcga tgaaaaacat cgccgcgccg
     3301 aagaacagca cgccggagat gcccacctgg ttgacgatgc ggtcggtgat gagcggaccc
     3361 acgagcgcgc cggtggtgcc gcccgcggcg atgaaaccga acagccgctt ggtctgctcg
     3421 ctcctgaaca gctccagcag gaagctccag aacaccgaga tcaggaacag attgatgacg
     3481 ctgatgccga cgtagaagac gcgcccgacg accatgttct ccggatcggc ccggaacacc
     3541 atgccggtgg cggccagcgc aatcgccacg ctgccgtaga tggccggcac cagcgtcgcg
     3601 cggctcacgc gcgccacgag gtagccgtac accgggatga ttgcgatcgc gaacaccgcg
     3661 gtccagatcc aggtgtcgct ggtgatcgcc tcgccgagga tggtgcccac ggtttcgcga
     3721 atgggccgga ccgcgaaata gccgcccatc acgcagaaca tcagcgcgaa cgcggtgaac
     3781 accgcgcgtt tctcaccggg ctcgatgcgg acgaaacgtg cgaggaagac gtcgagctgt
     3841 tgagtcatgc gtggagattc cgcgggcggt ttgttagcgc ccgattctag gcattagtcc
     3901 cgtgacaagg cgggcaaatt cgccaggtcg atcgtcagac cgggccggct gacggtcgcg
     3961 cttgcaccgc gcccgcgcag tttttcggcg agcgcctccg cggagggcac ttcgccgtgc
     4021 acgagatata ccggaggccg cgctgcgaag ctgtcatacc aacgtagcag atcgtcctgg
     4081 tcgccatgcg ccgagagtcc gcccagcgta tggatctgcg cgaggcaacg cacgcgctgg
     4141 ccgtggatga aaacctccgg gcggcggtcg acgagctgac ggcccagcgt tccgggcgcc
     4201 tggaatccca cgatgaccac gtggcactcg gcccgctcga ggttgtgctt gaggtgatgc
     4261 aatacgcgac cgccgttcgc catgcccgag ccggcaatga tgagcgcgcc gctgcgcagg
     4321 tggttgatgg cgcgggaatc atcggccgat tccgacaata cgagattcgg cagcggcggc
     4381 atgccgcgga agctctcgcg caggcgcgtg gtctcttcat cgaagcggtc gggattgctc
     4441 cagtacacgc ggctggcggc gatggccatg ggactgtcga ggaaaatctt ccagcgtgtc
     4501 aggtgccatt cgccgtagtg cttcgccagt agatagagca gctcctggct gcggccgatg
     4561 gcgaacgcgg gcacgatgac attgccgccg tcgcgtgctg cctgctcgag gattccgccg
     4621 agttcacgct ccgtgtcttc gcggccgcga tgctggcgat ttccgtaagt gctctcgatg
     4681 agcacgagat cggccgattc gaatttgtac ggatcctgca ggatg
//
LOCUS       DATDEU010000511         2819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_973232, whole genome shotgun sequence.
ACCESSION   DATDEU010000511 DATDEU010000000
VERSION     DATDEU010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973232"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="VIV63_12255"
     CDS             <1..1564
                     /locus_tag="VIV63_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6125415.1"
                     /translation="ERTEVEFECIRDGVERRFETRIVPYDRGRLLLVLRDVTERHRAA
                     NRIRELAFFDPLTQLPNRQYLLQLLEEARREAATTGERFAVVRINLDQFKRINDSIGH
                     GAGDALLQAVALRLGTFLKLESDGAVTMPLARLAGDEFAVAIRHLQSDADVTKAIANI
                     TAAFASPYLIRHRDFFVTCTIGIAIFPDHGSFADELLRTAGLALHEAKTSGGNRGVIY
                     SEHMRARSVERLELETELRRALEQNELFLVYQPQIEVATGRIASVEALVRWRHPTRGI
                     VPPDEFIPVAEESGLIVQLSDQVMRQALKQISEWWQAGCRDLRVAVNVSGAQFEAPGF
                     PDWVFAHLDEAGLPGACLELEITESLLMVDEAAAALAVKAVRELGVHVAIDDFGTGYS
                     SLAYLKHFPIEALKIDRSFVADLGRDSNDAAICAAIIAMGRQLGLKIVAEGVETQEQL
                     QFLATHGCTLAQGFYIARPVEALEMAKMLRIGADGTGIIPVAVPAKKNTEVPLADYEA
                     ELLIKATRAKAK"
     gene            1622..2407
                     /locus_tag="VIV63_12260"
     CDS             1622..2407
                     /locus_tag="VIV63_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY6125416.1"
                     /translation="MTGSLSVFWLGFLGSLLAGLGTSLGALGIFLVRRPSDRAQDGML
                     SAAAGIMLAATFFSLLLPALEQADELTTNRYLGPALVGLGVLLGAGGLFAIHRVVPHE
                     HFVLGKEGPENDRVARIWLFIIAITLHNFPEGMAVGVGFAGGEISNGTALAVGIGLQN
                     VPEGFAVALSLIAIGYSRSHAFWVATLTGLVEPVGGAMGAAAVSLAEPLMPIILGMAA
                     GAMLFVISDEIIPETHRRGYENVATFSLLGGFVVMMFLDTVFA"
     gene            complement(2412..>2819)
                     /locus_tag="VIV63_12265"
     CDS             complement(2412..>2819)
                     /locus_tag="VIV63_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125417.1"
                     /translation="MIRPAQADVLSSMMPPAPRKFAVTVVGNAAGDAATDEARTVAAG
                     ATDAQSTPLGRLYPKDLYSLSHIALPFPPRDGLYGSDPVATEDFGVRLGMVAVRGERG
                     ALIVSADTLMRASSNPFFDYMMGRIEAEIPRDQ"
BASE COUNT          471 a          957 c          894 g          497 t
ORIGIN      
        1 cgagcgcacc gaagtggaat tcgaatgtat ccgcgacggt gtcgagcgcc gtttcgaaac
       61 gcgcatcgtg ccctacgatc gcggccgact gctgctcgtg ttgcgcgacg tgaccgaacg
      121 ccatcgcgcc gcgaatcgca ttcgtgaact cgcgttcttc gatccgctga cgcagttgcc
      181 caaccggcag tacctgctgc agttgctcga agaagcacgt cgcgaagcgg ccactaccgg
      241 cgaacgcttc gccgtggtgc gcatcaacct cgaccagttc aagcgcatca acgacagcat
      301 cggccacggc gccggcgacg cgctgctgca ggccgtggcg ctgcgcctcg gcacgttcct
      361 caagctcgaa agtgatggcg cggtcaccat gccgctggcg cgactggcgg gcgacgaatt
      421 cgcggtcgcg atccggcacc tgcaatccga tgccgacgtg accaaggcca tcgccaacat
      481 cacggcggcg tttgcctcgc cctatctcat ccggcatcgc gacttcttcg tgacctgcac
      541 catcggcatc gcgatcttcc cggaccacgg aagtttcgcc gacgaactgc tgcgcaccgc
      601 cggcctggcg ctgcatgaag cgaagacgtc gggcggcaat cgcggcgtca tctacagcga
      661 gcacatgcgt gcccgctcgg tggaacgcct ggaactcgaa accgaattgc ggcgcgcgct
      721 cgagcagaac gaactgttcc tcgtctatca accgcagata gaagtcgcga cgggccgcat
      781 cgccagcgtc gaggcgctgg tgcggtggcg gcatcccacg cgcggcatcg tgccgccgga
      841 tgagttcatc ccggtggccg aagagtccgg cctgatcgtg cagctttccg accaggtcat
      901 gcgccaggcg ctcaaacaga tctccgagtg gtggcaggcg ggttgccgcg atctgcgcgt
      961 ggcggtgaac gtctcgggcg cgcagttcga agcgccgggc tttcccgatt gggtgtttgc
     1021 gcatctcgat gaagccggat tgcccggtgc ctgtctcgag ctcgagatca ccgagagcct
     1081 gctcatggtc gacgaagccg ccgcggcgct cgcggtgaag gccgtgcgcg agctcggcgt
     1141 ccacgtggcc atcgacgact tcggcacggg ttactcgtcg ctcgcctacc tcaaacattt
     1201 tcccatcgag gcgctcaaga tcgaccgctc gttcgtggcc gacctgggtc gcgattccaa
     1261 cgacgccgcg atctgcgcgg ccatcatcgc catgggccgg cagttgggcc tgaagatcgt
     1321 ggccgaaggc gtcgagaccc aggagcagct gcagttcctg gcgacgcatg gctgcacgct
     1381 ggcgcagggc ttctacatcg cgcgaccggt cgaggcactc gagatggcga agatgctgcg
     1441 catcggtgcg gacggcaccg gcatcatccc ggtcgccgtg ccggccaaga agaacacgga
     1501 agtacctctg gccgactacg aagccgagtt gctgatcaag gcgacccgcg ccaaggccaa
     1561 gtaatgggga cactccccat ttcctagcaa attgggccgc acctcgcgct acgatcgcgc
     1621 gatgaccggc tcactcagcg tcttctggct cggattcctc ggcagcctgc tcgccggcct
     1681 cggcacttcg ctcggtgcac tcggcatctt cctcgttcgc cgcccgagcg accgcgccca
     1741 ggacggcatg ttgagcgccg ccgcgggcat catgctcgcc gccaccttct tctcgttgct
     1801 gctgccggcg ctcgaacagg ccgacgagct caccaccaat cgatatctcg gaccggcact
     1861 ggtgggcctc ggcgtcctgc tgggagccgg cggattgttc gcgattcacc gcgtggtgcc
     1921 gcacgaacat ttcgtgctcg gcaaggaagg ccccgaaaac gatcgcgtcg cgcgcatctg
     1981 gctgttcatc atcgcgatca cgttgcacaa ctttcccgaa ggcatggcgg tgggcgtggg
     2041 cttcgcgggc ggcgaaatca gcaacggcac cgcgctcgcg gtcggcatcg gcctgcagaa
     2101 cgttcccgaa ggtttcgcgg tggcgctgtc gctgatcgcg atcggctatt cgcgctcgca
     2161 cgcattctgg gtggcgacgc tgacgggtct cgtcgaaccc gtgggcggcg cgatgggcgc
     2221 tgcggccgtg tcgctcgccg aaccgctgat gccgatcatc ttaggcatgg ccgcgggcgc
     2281 gatgttgttc gtgatcagcg acgagatcat tccggagacg caccgccgcg gttacgaaaa
     2341 cgtggccacc ttctcgttgc taggcggctt cgtggtgatg atgttcctcg ataccgtttt
     2401 cgcctagcgg atcactgatc gcgcggaatc tccgcttcga tgcgccccat catgtagtca
     2461 aaaaacgggt tcgagctggc gcgcatcagc gtatcggcac tcacgatcag cgcgccgcgt
     2521 tcgccgcgca cggccaccat gccgagccgc actccaaagt cttccgtcgc caccggatcg
     2581 ctgccgtaca gcccgtcccg cggcggaaac ggcagcgcga tgtgcgacag cgaatacaaa
     2641 tccttgggat agagccggcc caacggcgtg gactgtgcat ccgttgcgcc cgccgcgacc
     2701 gtgcgcgcct catcggtcgc ggcgtctccc gcggcgttac ccaccaccgt caccgcgaac
     2761 ttgcgcggcg ccggcggcat catcgacgac agcacatccg cctgcgcggg acggatcat
//
LOCUS       DATDEU010000512         4419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1556745, whole genome shotgun sequence.
ACCESSION   DATDEU010000512 DATDEU010000000
VERSION     DATDEU010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556745"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VIV63_12270"
     CDS             complement(<1..940)
                     /locus_tag="VIV63_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY6125418.1"
                     /translation="MKKNHVANVVLCAVFALTAACGKPDATATAAKPIQLDAPHLGLI
                     PAPLHSNGAIGTFRVAATTDILYSGGEGASGAAQYFVELIKQQRSDLAFRAPREESPD
                     SGSIAFVLTPDEASHGNEGYSLSVTPKGVTITARTSAGLFYGAVTLWQLITADPMQGL
                     SVDIPALRITDAPRFAWRGLMLDSARHFQSPEYVKQFIDWMALHKLNVLHWHLVDDQA
                     WRLEIKKYPKLTSVGAWRIPAGPAALADIDTATGKPRQYGGFYTQEQVWDIVAHAAKR
                     HVTVVPEIEMPGHATAAVVAYPELGVTNKPPKVVPEE"
     gene            complement(937..2526)
                     /locus_tag="VIV63_12275"
     CDS             complement(937..2526)
                     /locus_tag="VIV63_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6125419.1"
                     /translation="MPVTRPDIPRPDILIVGGGTAGWLTAAFLAKRLGGGPDGIRITL
                     IESSEIPTIGVGEGTFPTIAKTLSSLGVDECAFMRESGAAFKQGIKFVDWGCTPDNGR
                     HSHYFHPFALPREPDGLDLLPYWLAGEAGGASYADAVTLQERVCSAGRAPKRATDAEY
                     RGPMNYAYHLDAARFGQFLSGVAKAAGVAHLVGTVDDVELDEQGAIASVITREHGRLT
                     AGLYIDCTGFQSRLIGRAMGVPFHDKNDVLFVDRAVAVQVPYENENAAIPPYTISTAH
                     EAGWTWDIALRQRRGIGYVYSSGHTDETRAESVLRDYVGPAARDLPVRHLKMRVGWRE
                     RHWVKNCVAVGLSGGFLEPLESTGIILIEAAAHLIASFYQRSGGFEPAAGQFNSLMSR
                     RYERIVDFIKMHYFLTRRADTAFWRDNAHPSSAPESLRAHLEMWRHRPPTRLDFVMDY
                     ESFAPANYQFVLYGMGFRTPPGQTDDRLAARARQEFARVRDAAQRAAVALPPHRELLE
                     RVYQAGFSFSDLGRLPGASRQ"
     gene            complement(2557..>4419)
                     /locus_tag="VIV63_12280"
     CDS             complement(2557..>4419)
                     /locus_tag="VIV63_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6125420.1"
                     /translation="DNFNHNYMAWFSNMFGAGEQPTNTTVNDGGTLVGGTFANSANGW
                     GVVYDAILRDAQTETQGIDFDATFHSSDTLTWHTKIGYTEAEGATSVQPFWETQAHTG
                     FTWDFSHGVPEIDFTNITDTTDPASLPTLGWASQNQFLNEDDEFYVYGDGEIKLGSDG
                     KSSIKFGAKYTDHTRDVNITYGQTRGLLGATGCDGAPCSLAFAAGDTTPGDFLGDIAR
                     PGTVDAYRLVSEARLRQIYGALDFIQYDPNDPNIVANWPNFPTYHFGPLESFTVNEKT
                     FGGFAMATFAGDGVRGNVGIRVVSTKQVSDGWQVGVSASTPGATNNPFGMIAPLSIDN
                     DYTDVLPSANLVLDLSDDFLLRFGVSRVMARPEYNRIAPTITSFTPLLFTGTGGNPNL
                     EPYRANQLDLSAEWYFAESSLLSAALFYKDVESYITNGTGPERLPTEIVDPNDSRLSD
                     ANADCQNIGTALYNCIYQIDRPVNGEGGDIQGVELSWQQPIFGGFGVIMNYTYSDASS
                     DGDEPVPGNSKNNANLTAYFENERFGARLAYNYRSEYFIDNDRGRQLYADTTDSLDLS
                     VNVNLTDNFALTFDGVNLLDEELFQYYDNDKTRPARYYDNGPIYYFGVRVKFGQ"
BASE COUNT          767 a         1380 c         1443 g          829 t
ORIGIN      
        1 attcctcggg caccaccttc ggcggcttgt tggtcacacc gagctcggga tatgcgacca
       61 ccgcggcggt ggcatgtccc ggcatctcga tttcaggcac gacggtgaca tgtcgtttcg
      121 ccgcgtgcgc gacgatgtcc cacacctgct cctgcgtgta gaagccgccg tactggcgcg
      181 gcttgcccgt ggccgtgtcg atatccgcca gcgccgccgg ccccgccggg atgcgccacg
      241 cgcccaccga ggtgagcttg ggatatttct tgatctcgag gcgccaggcc tggtcatcga
      301 cgagatgcca gtgcaacacg ttgagcttgt gcagcgccat ccagtcgatg aactgcttta
      361 cgtactccgg ggactgaaaa tgccgcgcgg aatcgagcat gagcccgcgc cacgcgaaac
      421 gcggcgcgtc ggtgatgcgc agtgcgggaa tgtccaccga caggccctgc atcgggtccg
      481 ccgtgatgag ctgccacaac gtgaccgcgc catagaagag tcccgcggaa gtgcgcgccg
      541 tgatggtgac gcccttgggc gtgaccgaca gcgaataacc ttcgttgcca tgactcgctt
      601 cgtccggcgt gagcacgaag gcgatgcttc ccgaatccgg cgactcttca cgcggcgcgc
      661 ggaacgcgag gtcggaccgc tgttgtttga tgagctcgac gaaatactgc gcggcgccac
      721 tcgcgccctc gccacccgaa tagagaatgt cggtggtcgc agccacgcgg aaggtgccga
      781 tcgctccgtt gctgtggagc ggcgcgggta tcagacccag atgcggtgca tcgagctgaa
      841 tgggcttcgc cgccgtcgca gttgcatcgg gcttgccgca ggccgcggtc aacgcaaaca
      901 ccgcgcacag cacgacgttc gcaacatggt tcttcttcat tgtctgctcg cccccggaag
      961 ccgacccagg tcggaaaaac tgaatcctgc ctgatatacg cgctcaagta actcgcggtg
     1021 cggcggcagt gccacggccg cccgttgcgc ggcatcgcga acccgcgcga attcctggcg
     1081 cgcgcgcgcc gccagccggt catccgtctg gcccggtggc gtcctgaacc ccatgccgta
     1141 gagcacgaac tgatagttcg caggcgcgaa ggactcgtag tccataacga aatcgaggcg
     1201 cgtcgggggt cgatggcgcc acatttccag gtgtgcccgc agactttccg gcgcgctcga
     1261 cggatgagca ttgtcgcgcc agaacgccgt gtcggcgcgc cgggtcagga agtaatgcat
     1321 cttgatgaag tcgacgatgc gttcgtaccg ccgcgacatg agcgaattga actgccccgc
     1381 cgcgggttcg aacccgccac tgcgttgata gaagctcgcg atcaggtgag ccgcggcttc
     1441 gatgaggatg atgccggtgg attccagcgg ctcgagaaac ccgcctgaca atccgacggc
     1501 cacgcagttc ttcacccagt gccgctcacg ccagcccacg cgcatcttga gatgacgcac
     1561 cggcagatcg cgggccgcgg gtcccacgta gtcgcgcagc acactctcgg ctcgcgtctc
     1621 gtcggtgtga ccggagctgt agacgtagcc gatgccgcgc cgttggcgaa gggcaatatc
     1681 ccaggtccag ccggcttcgt gggccgtgga gatggtgtac ggcggaatgg ctgcgttctc
     1741 gttttcgtac ggcacctgca cggcgacggc gcggtcgacg aagagcacat cgttcttgtc
     1801 gtggaacggc acgcccatcg cgcgtccgat gagccggctc tggaatccgg tgcagtcgat
     1861 gtacaggccg gcggtcaggc ggccgtgctc gcgcgtgatc accgaggcga tggccccctg
     1921 ctcatcgagt tcgacgtcat cgacggtacc gaccaggtgc gccacacccg cagccttcgc
     1981 gacgccactc agaaactgac cgaaccgcgc ggcatcgagg tgataggcgt agttcatggg
     2041 gccgcgatat tcggcatcgg tcgcgcgctt gggtgcacgt cccgcgctgc aaacgcgttc
     2101 ctgcagcgtc accgcgtccg cataagacgc tccgcccgct tcaccggcca gccagtaggg
     2161 cagcagatcc agcccatccg gttcacgcgg cagagcgaac ggatggaagt agtgagaatg
     2221 ccggccattg tccggcgtgc atccccagtc gacgaacttg atgccctgct tgaacgccgc
     2281 gccgctttcg cgcatgaacg cgcactcgtc gacacccagc gaggacaggg tcttggcgat
     2341 cgtcggaaac gtgccctcac ccacaccgat cgtcggaatc tcgctggatt cgatcagcgt
     2401 gatgcgaatg ccgtcggggc cgccaccgag acgtttggcc aggaacgcgg ccgtgagcca
     2461 tccggcggtg ccaccgccca cgatcagaat gtcgggtcgg ggaatgtcgg gccgggtgac
     2521 tggcacccgg ccctcttcat gcctcatgac tcgacgttac tgcccgaact tcacgcgtac
     2581 gccgaagtag tagatagggc cgttgtcgta gtaacgcgcc gggcgcgtct tgtcgttgtc
     2641 gtagtactgg aacagctctt catcgagcag gttgacgcca tcgaaggtca gcgcgaagtt
     2701 gtccgtgagg ttcacgttga cggacaggtc gaggctgtcg gtggtgtccg cgtacaactg
     2761 ccggccgcga tcgttgtcga tgaaatactc ggagcggtag ttgtaggcca ggcgcgcacc
     2821 gaagcgttcg ttctcgaagt acgccgtgag gttcgcgttg ttcttcgaat tgccgggcac
     2881 cggctcgtcg ccatccgaac tcgcatcgga gtaggtgtag ttcatgatca cgccgaagcc
     2941 gccgaaaatc ggctgctgcc acgacagttc cacaccctgg atgtcgccac cctcgccgtt
     3001 caccggacgg tcgatctggt agatgcagtt gtacagcgcg gttccgatgt tctggcaatc
     3061 cgcattcgca tcgctcagcc gtgagtcatt gggatcgacg atttccgtcg gcaggcgctc
     3121 aggccccgtg ccgttggtga tgtacgactc cacgtcctta tagaagagtg cggccgagag
     3181 cagtgagctc tccgcgaagt accactcggc cgacaggtcg agctgattcg cgcgatacgg
     3241 ttcgaggttc ggatttccgc ccgtgccggt gaacagcagc ggcgtgaacg acgtgatggt
     3301 cggcgcgata cggttgtact cgggccgggc catgacgcgt gacacaccga agcgcagcag
     3361 gaagtcgtcc gagagatcga gaaccaggtt ggcgctcggc aacacgtcgg tgtagtcgtt
     3421 gtcgatggac agcggcgcga tcatgccgaa cggattgttg gtggcgcccg gcgtgctggc
     3481 cgaaacgccg acctgccagc cgtcggaaac ctgcttcgtc gacacgacgc gaatacccac
     3541 gttgccgcgg acgccgtcgc cggcgaaggt ggccatggcg aagccgccga aggtcttttc
     3601 gttgacggtg aagctctcga gcggcccgaa gtgatacgtc gggaagttag gccagttggc
     3661 gacgatgttc ggatcgttcg ggtcgtattg aatgaagtcg agcgcgccgt agatctggcg
     3721 caaccgcgct tcggagacga ggcgatacgc atcgaccgta cccggccgcg cgatgtctcc
     3781 gaggaagtcg cccggcgtcg tatcacccgc cgcgaatgcc agcgagcagg gagcgccatc
     3841 gcaacccgtg gcgcccagaa ggccgcgcgt ctggccgtag gtgatgttca cgtcgcgggt
     3901 atggtcggtg tacttcgcgc cgaacttgat cgagctcttt ccatccgagc ccagtttgat
     3961 ttcgccgtcg ccgtagacgt agaactcgtc gtcttcgttg aggaactgat tctgcgaagc
     4021 ccagcccagt gtcggcagcg acgccgggtc ggtggtgtcg gtgatgttgg tgaagtcgat
     4081 ttccggcacc ccgtggctga agtcccacgt gaagccggtg tgcgcctgcg tttcccagaa
     4141 cggctggacg ctggtcgcgc cttcggcttc ggtgtagccg atcttggtat gccaggtcag
     4201 cgtgtcgctg ctgtggaacg tggcatcgaa gtcgatgccc tgcgtttctg tctgcgcatc
     4261 gcgaaggatg gcgtcataca ccacgcccca gccgttggcg ctgttggcga acgtgccgcc
     4321 gaccagcgtg ccgccgtcgt tcaccgtggt gttggtgggc tgctcgccgg cgccgaacat
     4381 gttgctgaac cacgccatgt agttgtggtt gaagttgtc
//
LOCUS       DATDEU010000513         5376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1848233, whole genome shotgun sequence.
ACCESSION   DATDEU010000513 DATDEU010000000
VERSION     DATDEU010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848233"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2771
                     /locus_tag="VIV63_12285"
     CDS             <1..2771
                     /locus_tag="VIV63_12285"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY6125421.1"
                     /translation="GEIEFNPFTLVLKVHGLSFPDTEGQPLVGFRELLVDLNVSTLFR
                     LAPSFAAIELGEPFTSVVVRKDGSLNLVDLARPFATPEEKAAEAAGEPLKLYIERLGV
                     RSGRIDFEDRARPSVFHTRLAPITFELRDFATTGEAGNAYSLRAASINDERFSWDGTF
                     ETTPFTSTGKFEVANLRATTLWSYLRDALPFELTSGTINLSGGYDFAARESGLKLNVT
                     DVTATEVALQGRDQPPDDVKLAKLQVTNTRFDLRQRRVDVEKLTLSGAALRARRDAQG
                     HINLLALLGEEKVGVEAPPPAETPAAGEAASPWVLSAPDIALEGASMEVEDALVNPAA
                     TFTLAPIDVKVAGYSNAPDTKVQIDAAAGIDGKAKFAAKGEVALDTAALSMHVALSDF
                     NLASLQPYLGAYTQMTLSRGALTAALDVKRSEKSALDISGSVDVAKLHTIDNALKQEF
                     ITWERLRLTGLEYSTEPASLRIATVAANAPYVRLIIAPDQSINVSKVLSAPSGSMPTP
                     IQTVKTDADSRIAAPPPMRVSIGAVRIVNGAANFADFWIQPNYAVNLQNMNGSILGLS
                     SDPKSRAKVQFEGKVDRYAPARIDGDINLLSAALYTDLEVSFKGVEMTSVTPYSGRFA
                     GYKIEKGKLSIDVAYLVENRTLTAKQKFVIDQLELGERVESEDAVRLPLKLAVALLKD
                     RNGVIDIDLPMTGSLDDPQFRMGPLIWKAFVGLIGKAATAPFALLGRLFGGGEEMNLI
                     EFEPGSAALDPAAQEKIVAITRALTERPGLKLDVPTTYSPELDRASINAQRLEGLLRT
                     LPKSDELALADPAQRFELLLTQYRADYGARTALPPATLALNALRKSERMPEAFTAANQ
                     ELEQAIVQKHAAADTDLEQLGQMRARAIQDALLGGGTLDATRVFILGANPSAPTENQK
                     VRLELSLK"
     gene            3047..3847
                     /locus_tag="VIV63_12290"
     CDS             3047..3847
                     /locus_tag="VIV63_12290"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY6125422.1"
                     /translation="MKKQWKKSLTVAAIVCAGPASAIPVTFDFSGTVAQVITTDFAGG
                     LPTFDLSTAGQAFNAQFLIDTDLFSPLAAPPTTIGDRLAYNSELPGAVTSSLVVNGES
                     IDLAPYARNRATASFIDSYGIVSCGENCSRLGPDQFNVNTVSEDPTPLGPAASRTLSF
                     SFVAGYQSFDNPESALTWFDFSPDFDLTRIGSLPLLDSLLTTVTLTDFLSDCTDLRCQ
                     ATATRRTMFQVTSTTRTVASVPEPGTLGLLAMGLAGAWFMRRRRPQPV"
     gene            complement(3844..>5376)
                     /gene="hutU"
                     /locus_tag="VIV63_12295"
     CDS             complement(3844..>5376)
                     /gene="hutU"
                     /locus_tag="VIV63_12295"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY6125423.1"
                     /translation="VVYGGIGKAARDWACFDAILESLKKLEADETLLIQSGKPVGVFE
                     THADAPRVLLANSNLVPRWSTWEHFNELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYE
                     TFVEMGRQHYGGSLAGKRLLTAGLGGMGGAQPLASVMAGASCLAIECQRSRIDMRLKS
                     GYLDHATDNVDEALNIIESSCTERKPVSVGLLGNAGEVLPELYRRGVRPDLLTDQTSA
                     HDPINGYLPIGWTVEKWIEKRATDPAVVSAAARASMASHVHSMLDFQKAGVPTVDYGN
                     NIRQVALEEGVKNAFDFPGFVPAYIRPLFCRGVGPFRWVALSGDPEDIAKTDAKVKEL
                     LPGDANLHRWLDMARERIKFQGLPARICWVGLGDRHRLGLAFNEMVARGELKAPIVIG
                     RDHLDSGSVASPNRETEGMRDGSDAVSDWPLLNALLNTASGATWVSIHHGGGVGMGFS
                     QHAGVVIVCDGTPAAAKRIARVLWNDPATGVMRHADAGYEIAIDAARGHQLDLPGILP
                     RS"
BASE COUNT          953 a         1778 c         1697 g          948 t
ORIGIN      
        1 tcggcgaaat cgagttcaac ccgttcacgc tggtgctgaa ggtccacggc ctgtctttcc
       61 ccgacaccga agggcagccg ctggtcgggt ttcgcgagct gctcgtggat ctcaacgtct
      121 cgaccttgtt tcgcctcgcg ccgagtttcg ccgccatcga actcggtgag ccgttcacga
      181 gcgtggtggt gcgcaaggac ggttcgctca atctcgtgga cctggcgcgg ccgttcgcaa
      241 cccctgagga aaaagccgcc gaggctgccg gtgagccgct gaaactctac atcgaacggc
      301 tcggcgtaag gtcgggccgc atcgatttcg aagaccgcgc gcggccctcg gtgtttcaca
      361 cgcggctcgc gcccatcacc ttcgagctgc gcgacttcgc gacgacgggc gaagcgggta
      421 atgcctactc gctgcgcgca gcgtcgatca acgatgaacg attctcgtgg gacggcacgt
      481 tcgagaccac gcctttcacg tccacgggaa aattcgaagt cgccaacctg cgcgccacca
      541 cgttgtggtc atacctgcgt gacgcgctgc cgttcgagct caccagcggc acgatcaacc
      601 tttcgggcgg ctacgatttc gcggcacgcg agtcgggcct caagctcaat gtcacggacg
      661 tcaccgccac ggaagtggcg ctgcaaggcc gcgaccaacc accggacgac gtgaagctcg
      721 ccaagctgca ggtcaccaac acgcgcttcg atctgcgaca gcgccgcgtc gacgtggaga
      781 agctgaccct ttcaggcgcg gcgttgcgcg ccaggcgcga cgcgcagggg cacatcaacc
      841 tgctcgcgct cctgggtgag gagaaggtcg gcgtcgaggc gccaccgcca gccgaaacgc
      901 cggctgccgg cgaggccgct tcgccctggg tattgagcgc accggacatc gcgctcgaag
      961 gcgcgagcat ggaggtcgaa gacgcgctcg tgaatccggc ggcgacattc acgctcgcgc
     1021 ccatcgatgt gaaagtggcg ggctacagca atgcccccga caccaaagtg cagatcgacg
     1081 cggccgcggg catcgacggc aaggcgaagt tcgccgcgaa gggcgaagtc gcgctggata
     1141 ccgcggcgct gtcgatgcac gtggccttga gcgatttcaa tctcgcgtcg ctgcaaccgt
     1201 atctcggcgc ctatacccag atgacgctgt cgcgaggcgc gctcacggcg gcgctcgacg
     1261 tcaaacgcag cgagaagagc gcgctcgaca tctcgggcag cgtcgacgtg gcgaaactgc
     1321 acaccatcga taacgcgctg aagcaggaat tcatcacctg ggagcggctg cgactcacgg
     1381 gtctggaata ttccacggag cccgcgagcc tgcgcatcgc caccgtcgcg gcgaatgcgc
     1441 cctatgtgcg cctcatcatc gcgccagacc agtccatcaa cgtcagcaag gtgttgagtg
     1501 cgccgtcggg cagcatgccg acgcccattc aaacggtgaa aaccgacgcg gactcgcgaa
     1561 tcgccgcgcc gccgccgatg cgcgtgagca tcggcgccgt acgcatcgtc aacggcgccg
     1621 ccaacttcgc cgacttctgg atccagccta actacgccgt caacctgcag aacatgaacg
     1681 gcagcattct cgggctgtcg tcggacccga agtcgcgcgc caaggtccag ttcgaaggca
     1741 aggtcgaccg ttatgcgccg gccaggatcg acggcgacat caacctgttg tccgctgcgt
     1801 tgtacacgga tctcgaggtg agcttcaaag gcgtcgagat gacctcggtg acgccgtact
     1861 cgggccggtt cgccggctac aagatcgaga agggcaagtt gtccatcgac gtggcctatc
     1921 tcgtggagaa ccgcacgctt accgcgaagc agaaattcgt catcgatcag ctcgagctcg
     1981 gcgagcgcgt ggagagcgaa gacgccgtgc ggctgccgct caagttggcc gtggcgctgc
     2041 tcaaggatcg caacggcgtc atcgacatcg acctgcccat gaccggcagc ctcgacgatc
     2101 cgcagttccg catggggccc ctcatctgga aagcgttcgt gggtctcatc ggcaaggccg
     2161 ccaccgcgcc gtttgcgtta ctggggcgcc tgttcggcgg cggcgaggag atgaacctca
     2221 tcgagttcga gccgggcagc gccgcgctcg acccggctgc gcaggaaaag atagtcgcca
     2281 tcaccagggc gctcactgaa cggcccggcc tcaagctcga tgtcccgacc acctattcgc
     2341 cggaactcga tcgcgcttcg atcaatgcgc agaggctcga aggtctgctg cgcacattgc
     2401 cgaagtcgga tgagcttgcg ctcgccgatc ccgcccagcg attcgaactg ctgttgacgc
     2461 aataccgcgc cgattacggc gccaggaccg cgttgccacc ggccacgctc gcgctcaacg
     2521 ccctgcgcaa gagcgaacgc atgccggaag cctttacggc cgcgaatcag gagctcgagc
     2581 aggccatcgt gcagaaacac gcggcagcgg acacggatct cgagcagctc gggcagatgc
     2641 gcgcccgcgc gattcaggat gctttgctgg gaggcgggac gctcgatgcg acccgcgttt
     2701 tcatcctggg cgccaatcca tcggcgccca cggagaacca gaaggtgcgg ctcgagctca
     2761 gcctgaagta gctgggcccg gcggcaccgt cagtttctgg cgaattctca gggacgctga
     2821 aggaaggaac gctcggcttt cttggccttc ttcgcggcgc gcttttcatc cggcgtcttc
     2881 gccgccttct tcttcaggtt ctttttctgg tccatgccct tgctcatcgc tgcgctcctg
     2941 gtgggtgagg ggtggcgcga gggtagcagt tatgtaacgt ttgtgctgcg cccgcggcgc
     3001 tgcctgctag agtccgcgca ctccttcata gaagaaagag aacaacatga agaaacagtg
     3061 gaagaagtct ctaacggtcg cggccatcgt gtgcgccgga cccgccagcg ccatccccgt
     3121 cactttcgat ttcagtggta ccgtcgccca ggtcatcacc actgatttcg cggggggatt
     3181 accgacgttc gacctgtcga cggccggcca ggcgttcaac gcgcaattcc tcatcgacac
     3241 cgacctcttc agtcctctcg cggctcctcc gacaacgatc ggagaccggc tcgcatacaa
     3301 ctcggaattg cctggcgccg tgacttcgag tctcgtggtg aacggcgagt ccatcgatct
     3361 cgcgccgtac gccagaaacc gggcgaccgc gagtttcatc gactcctatg gcatcgtgtc
     3421 ctgtggcgaa aattgctcaa gactcgggcc ggaccagttc aacgtgaaca ccgtgtccga
     3481 agatccgacg ccgctgggtc ccgccgcgtc gcgcacgttg tcgttcagct tcgtggcggg
     3541 ctaccagagc ttcgacaatc ccgagtctgc cttgacgtgg ttcgacttct caccggactt
     3601 cgatctcacc cggatcggat cgttgccgct gctcgattcc ttgttgacca ccgtgacgct
     3661 gaccgatttc ctttccgact gcaccgatct gcggtgtcag gcgacggcga cgcggcggac
     3721 gatgttccag gtgacgtcga ccactcgcac ggtcgcctcg gttcccgagc ccggcacgct
     3781 gggcttgtta gccatgggcc tggcgggcgc gtggttcatg cggcgccggc ggccgcagcc
     3841 cgtctaactt cgcggcagga ttcccggcag atcgagctga tgcccgcgcg cggcgtcgat
     3901 cgcgatctcg taccccgcat ccgcgtggcg catgacgccg gtcgccggat cgttccacaa
     3961 cacgcgcgcg attcgtttcg cggcggcggg tgtgccgtcg cagacgatga ccacgccggc
     4021 gtgctgcgag aagcccatgc ccacgccacc gccgtggtga atcgacaccc acgtggcgcc
     4081 cgaggcggtg ttgagcaacg cattgagcag gggccagtcc gacaccgcat ccgagccgtc
     4141 gcgcatgcct tcggtctcgc ggttgggcga ggccaccgag cccgagtcca ggtgatcgcg
     4201 gccaatgacg atgggcgcct tgagctcacc cctggccacc atctcgttga aggcgaggcc
     4261 gagccggtgc cggtcaccga gacccaccca gcagatacgc gcgggcagac cctggaactt
     4321 gatgcgctcg cgcgccatgt cgagccatcg atgcaggttc gcatccccgg gcagcagctc
     4381 tttgaccttc gcgtcggtct tcgcgatgtc ttcgggatcg cccgagagcg ccacccagcg
     4441 gaacgggccg acaccgcggc agaacagcgg ccgtatatag gcggggacga acccgggaaa
     4501 gtcgaacgcg ttcttcacgc cttcttcgag cgccacctgg cggatgttgt tgccgtagtc
     4561 gaccgtggga acgccggctt tctggaagtc gagcatggag tgcacatgcg aggccatcga
     4621 cgccctggct gccgcgctca ccacggcggg atcggtggcg cgcttctcga tccatttctc
     4681 gacggtccag ccgatgggta gatagccgtt gatgggatcg tgcgcggaag tctggtccgt
     4741 cagcagatcg ggccgcacgc cgcgacggta gagttccgga agcacctcgc ccgcgttgcc
     4801 gagcagcccc acggacacgg gtttgcgctc ggtacaggag ctctcgatga tgttcagtgc
     4861 ttcgtcgacg ttgtccgtgg cgtgatcgag atagccggac ttcaggcgca tgtcgatgcg
     4921 cgagcgctgg cattcgatgg cgaggcagga cgcgccggcc atgaccgacg ccagcggctg
     4981 cgcgccgccc atgccgccga gaccggccgt caacagcctc ttgcccgcga gactgccgcc
     5041 gtaatgctgg cggcccatct cgacgaaggt ttcgtacgtg ccttgaacga tgccctgcga
     5101 cccgatgtag atccacgagc cggccgtcat ctggccgtac atcatgagtc ccttgcgatc
     5161 gagttcgttg aagtgttccc aggtgctcca gcgcggcacg agattggaat tggcgagcaa
     5221 cacgcgcggc gcatcagcat gcgtctcgaa cacacccacg ggttttcccg actggatgag
     5281 cagcgtctcg tcggcttcca gcttcttcag ggattccaga atggcgtcga agcacgccca
     5341 gtctcgcgcg gccttgccga tgccgccgta gacgac
//
LOCUS       DATDEU010000514         4827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2140210, whole genome shotgun sequence.
ACCESSION   DATDEU010000514 DATDEU010000000
VERSION     DATDEU010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140210"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VIV63_12300"
     CDS             complement(<1..441)
                     /locus_tag="VIV63_12300"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6125424.1"
                     /translation="MIILEVLLLVPGLVLALFGAYQVGLLLLSLVYNFLHRRDVLVTS
                     PPSRRIVVIVPAHNEELLIGRTVDSILGANYPRELLHLVVVADNCSDATASIVRAHGG
                     TCFERHDLEKRGKPYALDWTIRQLDLDAHDALVIIDADTEIHREF"
     gene            complement(458..1402)
                     /locus_tag="VIV63_12305"
     CDS             complement(458..1402)
                     /locus_tag="VIV63_12305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6125425.1"
                     /translation="MQSNAPTLSVVIGTLNRCDSLPRAIESLLNQQCPASRVEIIVVD
                     NGSTDATAEIARGYAAKHDRVRYVLEPKLGLSNARNAGIANARGLFVGFFDDDATAEP
                     QWAATILSIIDEDAEVGALGGPIVVEWPTGKPQWMPPTREGYYGHCYFGPTRRLLTFP
                     EYPFGSNMIIRRERLTRIGGLRDSVGPKGRNMMSAGEQDLFMRLTQEPLRIVYDPAAV
                     VHHWIFANQVTKQWLMRRSMKHGVSNTRMMNVIHARKRGYWFAQGTVAAWRCLVGAIS
                     GSVAWLLRRPPVVVTSRFATMMYWNGITRGSFANLFAR"
     gene            1784..2776
                     /locus_tag="VIV63_12310"
     CDS             1784..2776
                     /locus_tag="VIV63_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125426.1"
                     /translation="MYAGSAVFSEAQAQVATWFRADFNNLNQSGTNMYNFANRYAQSA
                     TTWQVAHTTNEGWNGTAAPRVSIHGCDQSSPTCNFSEHQFNAGWVTPNVGGSRAMGDS
                     VFIRWRIRFDPGTVFPPDTVNAKFILFGRTGTTPNSRWIIHLLSAKDNQGCTIGFDSY
                     SFMNWTPPANIWTRSSHWGLASDFNTGSVAGRYAGFSSHVNIDWSCAPAVLVTAANHA
                     SPVPKPQNVGAAPVNGWYHLQYQATSGADGQADFRIWANNNNQSTPSSEHINMASGLG
                     INGWDGDVNVVGYWGTNYTGTLRFVIDDFEIGPAFDPNWYPASGPRPSAPSQVN"
     gene            complement(2828..>4827)
                     /locus_tag="VIV63_12315"
     CDS             complement(2828..>4827)
                     /locus_tag="VIV63_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6125427.1"
                     /translation="LLRVAIFGGGRMAGNHATAIRLQPDTKFVAVADPYLSPEEVRER
                     FGADVEHFKDPQELLTRAKPDVVHIVTPPHTHYPLSRLALENGASVYVEKPFALSHRE
                     AAEILDLAASKGLQATAAHQVLFQRAGQLYQKYLPMLGKLIHVESYFSFKPVRRRADG
                     GTPLGAEDQLIDILPHPVYLLLSALESQSAETPELTALDVDADGEVRAVIRSGGVLAT
                     LIVSLRARPVESYLRVVGTNGSVTADFILGDVVALPGAGASGPAVVVKPFSQSFKKFW
                     GSFAGVGRLLFRKQKSYPGLGELLTLLYGSVRNGTNPPMSRQTILTTVSICEQISARL
                     YAAGAKVEEQARVTLAAANAALSPVDPARGTVFVTGGSGFLGRCALRDFRADGWRVRA
                     LVRRIPGARNQVPGIDYVEGDLGRGISPQLLEGVNVVAHLAAETAGNQQAHERNTVVA
                     TRNLIDAMKAAGVRKLINISSVAVLKPGPRLLREDSPVDHGNLSRGPYVWAKAEAEVD
                     AIKRAEAGDIEVRTIRLGPLVDFDDYTPPGRLGREVVRLFVAMGSRGNELSVCDVRTA
                     AQVVRHYANDFDAMPAMVNLLEVPATTRGDLANRLRAPRPDLKFFWMPFFVLRGLSWF
                     AIGLQKVLKPGRPALDLYAAFKSERYDPAIAERVIASARKK"
BASE COUNT          854 a         1546 c         1580 g          847 t
ORIGIN      
        1 gaactcgcga tggatttcgg tgtcggcgtc gatgatgacc agcgcgtcgt gcgcgtcgag
       61 gtcgagctgg cggatggtcc agtcgagcgc gtagggcttg ccgcgcttct cgagatcgtg
      121 gcgctcgaag cacgtgccgc cgtgagcgcg cacgatgctc gccgtggcgt cgctgcaatt
      181 gtcggcgacc accaccaggt gcagcagctc gcgcggatag ttagctccga ggatgctgtc
      241 gaccgtgcgg ccgatgagca gttcttcgtt gtgggccggc acgatgacca cgatgcgccg
      301 gctcggcggc gaggtcacca ggacgtcgcg gcgatgcagg aagttgtaga ccaggctgag
      361 cagcagcagg cccacctggt aggcgccgaa cagcgcgagc accaggcccg gcaccagcaa
      421 caggacttcg aggatgatca tgacgagcgt gatttttcta tcgggcgaac aggttggcga
      481 acgagccgcg agtgatgccg ttccagtaca tcatggtcgc gaatcgtgac gtcaccacga
      541 ccggcggcct gcgcagcagc caggcaacgg atccggaaat ggcgccgacc aggcagcgcc
      601 acgccgcgac cgttccctgc gcaaaccagt aaccgcgttt acgcgcgtgg atcacgttca
      661 tcatgcgggt attgctcacc ccatgcttca ttgagcgccg catcaaccac tgtttcgtga
      721 cctgattggc gaaaatccag tgatgcacca ccgccgcggg gtcgtagacg atgcgcaggg
      781 gttcctgcgt gagccgcatg aagaggtcct gctcgcccgc ggacatcatg ttccggccct
      841 tggggcccac tgaatcgcgc aggccgccga tgcgcgtgag gcgctcgcgg cggatgatca
      901 tgttgctgcc gaacggatac tcgggaaacg tcagcaaccg gcgcgtgggg ccgaagtagc
      961 agtggccgta gtagccctcg cgcgtcgggg gcatccactg cggcttaccc gtaggccact
     1021 cgaccacgat gggtccgccc agggctccca cctcggcgtc ttcatcgatg atggaaagta
     1081 tggtcgcagc ccattgcggc tcggccgtcg cgtcgtcgtc gaagaatccg acgaacaggc
     1141 cgcgcgcatt ggcgatgccg gcattgcggg cattagatag tccgagtttc ggctcgagga
     1201 cataacgcac gcggtcgtgt tttgccgcat agccccgggc gatttccgcg gtggcgtcgg
     1261 tagaaccgtt atcgacgacg atgatttcga cccgcgaggc gggacactgc tgattcagca
     1321 gactttctat ggccctgggc aggctgtcgc agcgattgag cgtgccgatg accaccgaca
     1381 gcgtaggagc attcgattgc atttcgttca tttggttgtg aggtctgcgt acgcaggaac
     1441 ccgcgtcggg cgaagcctta agtacgaaca gcagcgggcg cgatgatact ccaacgtcgc
     1501 cgggcgcaga cgccacggga ccccggcaga ttttgaaaaa tgagcgccat cgcccaaaca
     1561 accagccaag atagtgtttg actgcaatca cattatggcg aagatcggac cggtaccgga
     1621 gtgatcccgt gtcggttatg acagacaatt gtgatcaacc ttcaagccgg tattcttgcg
     1681 ctatcttcgc cggcgcacgt tacggccttt atcaatcctt gagtgagggc agacctaaat
     1741 gagtcctgca cgtttccttg ttctagcaac cgcagtgtta ggcgtgtacg ccggctccgc
     1801 ggtattttct gaagcgcaag cccaggtcgc cacctggttc cgtgcggact tcaacaatct
     1861 gaaccagtcc ggcacgaaca tgtacaactt cgcgaatcgt tacgcgcagt ccgccacgac
     1921 gtggcaggtt gcacacacga ccaacgaagg ctggaatggc accgccgctc cgcgcgtcag
     1981 catccatggt tgcgaccagt cgagcccgac ctgcaacttc agcgagcatc agttcaatgc
     2041 tggctgggtc acgcccaacg tcggcggctc gcgcgccatg ggtgactcgg tgttcatccg
     2101 ctggcgcatc cgcttcgatc cgggcaccgt gtttccgccg gacacggtga acgccaagtt
     2161 catcctgttc gggcgcacgg gcaccacccc gaactcgcgc tggatcattc acctgctctc
     2221 cgccaaggac aaccagggtt gcacgatcgg cttcgacagc tactcgttca tgaactggac
     2281 cccgccggcg aacatctgga cccgcagcag ccactggggt ctcgcgtccg acttcaacac
     2341 cggctcggtt gccggccgct acgcgggctt ctcgtcacac gtgaacatcg actggtcgtg
     2401 cgcaccggcg gtgctggtca cggcggcgaa ccacgcctct ccggttccga agccgcagaa
     2461 cgtcggtgcg gcgccggtca acggctggta tcacctgcag taccaggcga cctcgggcgc
     2521 ggacggccag gccgacttcc gcatctgggc caacaacaac aaccagagca cgccttcgtc
     2581 ggagcacatc aacatggctt ccggcctcgg catcaacggt tgggatggcg acgtgaacgt
     2641 ggtgggctac tggggtacta actacaccgg tacgctgcgc ttcgtgatcg acgacttcga
     2701 gatcggtccg gcgttcgacc cgaactggta cccggcctcg ggaccgcggc cgagcgctcc
     2761 gtcccaggtc aattaatcga gtcgatgaaa tcaaacggcg gtccgtcgca agacgggccg
     2821 ccgtttttca tttcttgcgt gccgaggcga tgacgcgctc ggcgatcgcc gggtcgtaac
     2881 gctccgactt gaatgccgcg tacaggtcga gcgccgggcg tccgggcttg agaaccttct
     2941 gcaggccgat cgcgaaccag ctcaggccac gcaacacgaa gaacggcatc cagaagaact
     3001 tgaggtccgg tcgcggcgca cgcagccggt tcgccagatc gccgcgcgtg gtcgcgggca
     3061 cttccagcag attcaccatc gccggcattg cgtcgaaatc gttggcgtag tgacggacca
     3121 cctgcgcggc agtgcgcacg tcgcagacgg acagttcgtt gccgcggctg cccatggcga
     3181 cgaacaggcg cacgacctcg cggccgagtc ggccgggcgg cgtgtagtca tcgaagtcga
     3241 ccagcggtcc cagccggatc gtgcggactt cgatgtcgcc agcctcggcg cgcttgatcg
     3301 catcgacctc ggcttcggcc ttcgcccaga cgtaagggcc gcgcgagagg ttgccgtgat
     3361 cgaccgggga gtcttcccgc agcaggcgag ggccgggctt gagcacggcc acgctgctga
     3421 tgttgatgag cttgcgtacg cccgccgcct tcatcgcatc gatgaggttg cgcgtggcga
     3481 cgaccgtatt acgttcgtgc gcctgctggt tgcccgcggt ttccgcggcc aggtgcgcca
     3541 ccacgttcac gccctcgagc aactgcggcg aaatgccgcg tccgaggtct ccttccacat
     3601 agtcgatgcc gggcacctgg ttgcgagcac cgggtatgcg ccgcacgagc gcgcgcacgc
     3661 gccagccgtc ggcgcggaag tcgcgcagcg cgcagcggcc gaggaagccc gaaccgccgg
     3721 tgacgaacac cgtgccgcgc gcgggatcga ccggtgagag cgccgcattg gcggcggcca
     3781 gcgtgacgcg cgcctgttct tcgaccttgg cgcccgcggc gtacagacgc gcggagatct
     3841 gctcgcagat ggagaccgtg gtcaggatgg tctgccggct catcggcggg ttcgttccat
     3901 tgcgcacgct gccgtacagc agcgtcagca gttcgcccag gcccgggtaa ctcttctgct
     3961 tgcggaacag caggcggccg acgccggcga agctgcccca gaatttcttg aaggactggc
     4021 tgaagggttt gaccaccacg gccggacccg aggcgcccgc gccgggcagc gccacgacgt
     4081 cgcccaggat gaagtccgcc gtcaccgagc cattggtgcc cacgacccgc agatacgatt
     4141 cgaccgggcg cgcgcgcagg gacacgatga gcgtcgccag cacgccgccg ctgcggatca
     4201 ccgcgcgcac ttcgccgtcg gcatcgacgt cgagcgcggt gagctccggg gtctcggcgg
     4261 actgcgattc gagcgccgac aacagtagat agaccgggtg cggcaggatg tcgatgagct
     4321 ggtcttcggc gccgagcggc gtgccgccgt cggcgcgccg gcgcaccggc ttgaacgaga
     4381 agtaactctc gacgtgaatc agcttgccga gcatcggcag atatttctgg tacagctggc
     4441 ccgcgcgctg gaacagcacc tggtgcgcgg ccgtggcctg caggcccttc gaggccgcga
     4501 ggtcgagaat ttcggccgct tcgcgatgcg agagggcgaa cggcttctcg acatagacgc
     4561 tggcgccgtt ctcgagcgcc aggcgcgaga gcgggtaatg cgtgtgcggc ggcgtgacga
     4621 tgtgcaccac gtcgggcttc gcgcgggtca gcagttcctg cgggtccttg aagtgctcga
     4681 cgtcggcgcc gaatctctcg cgaacttctt cggggctcag gtaggggtcg gcgaccgcga
     4741 cgaatttcgt gtccggctgc agccgtatgg ccgtggcatg gtttcccgcc atgcgtccgc
     4801 caccgaatat cgccactcga agtagtt
//
LOCUS       DATDEU010000515         3721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_828499, whole genome shotgun sequence.
ACCESSION   DATDEU010000515 DATDEU010000000
VERSION     DATDEU010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828499"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VIV63_12320"
     CDS             <1..193
                     /locus_tag="VIV63_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125428.1"
                     /translation="EAFYRDALPQAANLRSAFDAARAALAKRETAEGRIPSNPQAHFG
                     AAIERKLESFETKAVPSRP"
     gene            complement(168..1652)
                     /locus_tag="VIV63_12325"
     CDS             complement(168..1652)
                     /locus_tag="VIV63_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY6125429.1"
                     /translation="MGKPTGFIEYLRELPVDRSPGERVRDWNEFHHHMDEKRLRQQGA
                     RCMDCGVPFCHTGKLISGMASGCPVNNLIPEWNDLVFRGLWREALDRLHRTNNFPEFT
                     GRVCPAPCEGSCVLGINDPPVTIKNIENAIVDKGFEEGWIIAEPPTTRTGKKVAVIGS
                     GPAGLAAAAQLNKAGHSVTVLERADRPGGLLTYGIPNMKLDKREVVARRVSLLEKEGI
                     KFVCNATVGENVEAELLVKDFDATVICTGATVPRDLPVEGRNLNGVHFAMDFLTVSTQ
                     AVLKGAPESTPIYAKGKDVIVLGGGDTGTDCVGTSMRHGCKSITQIEIMAMPPMDRAA
                     DNPWPEWPKVYKMDYGQEEAAAKFGSDPRVYLTTVKKFKDDGAGNLAAVVTVNIQWEK
                     NDKGQFIPVEQPGSEKEHPAQLALLAMGFLGPEQALLKDLKVDTDARSNVKAEYGKYG
                     TTVPGVFAAGDARRGQSLVVWAINEGRGAARECDRWLMGATELP"
     gene            complement(1681..>3721)
                     /locus_tag="VIV63_12330"
     CDS             complement(1681..>3721)
                     /locus_tag="VIV63_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY6125430.1"
                     /translation="TLRGLLDFKQGESIPIEEVESVESLMKRFKTGAMSYGSISKEAH
                     ETLAIAMNRVGGKSNTGEGGEDPDRYVPMDNGDSKNSAIKQVASGRFGVTSHYLVNAK
                     ELQIKMAQGAKPGEGGQLPGTKVYPWIAKTRGTTAGVGLISPPPHHDIYSIEDLAELI
                     HDLKNANRDARISVKLVAEVGVGTIAAGVAKAHADVVLISGADGGTGASPLTSLIHAG
                     LPWELGLAETHQTLVINNLRSRIVVETDGQLKTGRDVAIAALLGAEEFGFATAPLVAV
                     GCIMMRVCHLNTCPAGVATQDPRLREKFAGKPEHAVNFMRFIATHLREIMAQLGFRKL
                     EDMVGRVDMLEPRAAIDHWKAKGFDFSNILYAPDAGPEIGRFKQIGQDHGIDKSLDVT
                     TLLGLCEPAIARGEKVSAELPVRNVNRVVGTITGSEVTKKWGVAGLPEDTIQIKFKGS
                     AGQSFGAFVPNGMSFTLEGDANDYFGKGLSGGKLAVFPSPLSTFPSEQNMIIGNVALY
                     GATKGEVFVRGMAGERFAVRNSGVDTVVEAVGDHGCEYMTGGRVVVLGATGRNFAAGM
                     SGGIAYVLDEHNEFPSRVNAQMVDVERVEDPKEAGELRDLIGKHVAATGSEHAKAILA
                     AWETKLPKFVRVIPKDYKRALACLKRAHDQGLSGDEAIMAAFEENARDLSRVGGN"
BASE COUNT          582 a         1226 c         1181 g          732 t
ORIGIN      
        1 cgaagcgttc tatcgggatg cactgcccca ggcggcgaac ctgcggagcg ctttcgatgc
       61 agcccgcgcc gcgctcgcga agcgcgaaac ggccgaagga agaatcccgt cgaatccgca
      121 ggcgcatttc ggcgccgcga tcgagcggaa gctggaatcc ttcgagacta aggcagttcc
      181 gtcgcgccca tgagccagcg gtcgcactcg cgtgcggccc cacggccttc gttgatggcc
      241 cagaccacca gcgactgacc ccggcgcgcg tcgccggcgg cgaacacgcc cggcacggtg
      301 gtgccgtact tgccgtactc ggccttcacg ttgctgcgcg catcggtgtc caccttgagg
      361 tccttgagca gcgcctgctc gggcccgagg aatcccatgg ccagcaacgc gagttgcgcg
      421 ggatgttctt tctcgctgcc cggctgctcg acgggaatga actgaccctt gtcgttcttt
      481 tcccattgga tgttcacggt gacgaccgcg gcgaggttgc cggcgccgtc gtccttgaat
      541 ttcttgaccg tcgtcagata gacgcgcgga tcggaaccga atttggcggc ggcctcttcc
      601 tggccgtagt ccatcttgta gaccttgggc cattccggcc acggattgtc cgcggcgcga
      661 tccatcggcg gcatcgccat gatttcgatc tgcgtgatcg acttgcagcc gtggcgcatc
      721 gatgtgccga cgcagtcggt gccggtgtcg ccgccgccca gcacgatgac gtccttgcct
      781 ttcgcgtaga taggcgtcga ttcgggagcg cccttgagca ccgcttgcgt gctcacggtc
      841 aggaaatcca tggcgaagtg cacgccgttg agattgcggc cttcgaccgg cagatcgcgc
      901 ggcacggtgg cgccggtgca gatgacggta gcgtcgaaat ccttgaccag cagttccgcc
      961 tcgacgtttt caccgacggt ggcgttgcag acgaacttga tgccttcctt ctcgagcagc
     1021 gaaacacggc gcgcgaccac ttcgcgcttg tcgagcttca tgttcgggat gccgtaggtg
     1081 agcagaccac cggggcgatc cgcacgctcg agcacggtca ccgagtgacc ggccttgttg
     1141 agctgcgcgg cggccgcgag tcccgccggt ccggagccga tgacggcgac cttcttgccg
     1201 gtgcgcgtgg tcggcggctc cgcgatgatc cagccttctt cgaaaccctt gtcgacgatg
     1261 gcgttttcga tgttcttgat ggtcaccggc ggatcgttga tgccgagcac gcacgaacct
     1321 tcgcagggcg ccgggcacac gcggcccgtg aactccggga agttgttggt gcgatgcagg
     1381 cgatcgagcg cctcgcgcca caatccgcgg aacacgagat cgttccactc agggatgagg
     1441 ttgttcaccg ggcagcccga ggccatgccc gagatcagct tgcccgtgtg gcagaacggc
     1501 acaccgcagt ccatgcaacg cgcgccctgc tgacgcagac gcttctcgtc catgtggtga
     1561 tggaactcgt tccagtcgcg cacgcgttcg cccggtgaac ggtccaccgg cagttcgcgc
     1621 aggtactcga taaatccggt tggctttccc atctatctag ttcttctcgt gattcttcgc
     1681 ttaatttccg ccgacgcggg agaggtcacg cgcgttctct tcgaacgccg ccatgatggc
     1741 ctcatcgccc gacaatccct gatcgtgtgc ccgcttgagg caggccagtg cgcgcttgta
     1801 gtccttgggg atcacgcgca cgaacttcgg cagcttggtt tcccaggccg cgagaatggc
     1861 tttcgcatgt tcgctgccgg tggccgcaac gtgtttgccg atgaggtcgc gcaactcgcc
     1921 ggcctctttc ggatcttcga cacgttcgac gtccaccatc tgcgcgttga cgcgcgaggg
     1981 gaactcgttg tgctcgtcga gcacgtaggc gataccgccc gacatgcccg cggcgaaatt
     2041 gcggcccgtc gcgcccagca ccacgacgcg tccgccggtc atgtattcgc aaccgtggtc
     2101 acccacggcc tcgaccaccg tgtccacgcc ggaattgcgc accgcgaacc gttcgcccgc
     2161 catgccgcgc acgaacactt cacccttggt cgctccgtaa agagcgacgt tgccgatgat
     2221 catgttctgc tcggacggga acgtcgacag cggcgacggg aacaccgcga gcttgccgcc
     2281 cgacagtccc ttgccgaagt agtcgttggc gtcgccttcg agcgtgaagc tcatgccgtt
     2341 gggcacgaac gcgccgaagc tctggcccgc cgaacccttg aacttgatct ggatggtgtc
     2401 ctcgggcagg cccgcgacgc cccacttctt cgtgacctcg ctacccgtga tggtgcccac
     2461 cacgcggttg acgttgcgca ccggcaattc agccgacact ttctcgccgc gcgcgatggc
     2521 cggttcgcac agacctaaca acgtggtgac gtcgagcgac ttgtcgatgc cgtgatcctg
     2581 gccgatctgc ttgaagcggc cgatctcggg gccggcgtcc ggcgcgtaga ggatgttgct
     2641 gaagtcgaaa cccttggcct tccagtggtc gatggcggcg cgcggttcga gcatgtcgac
     2701 gcggcccacc atgtcttcga gcttgcggaa gccgagttgc gccatgatct cgcgcaggtg
     2761 cgtcgcgatg aaacgcatga agttcacggc gtgctcaggc ttgccggcga atttctcgcg
     2821 caggcgcgga tcctgcgtcg ccacacccgc cgggcaggtg ttcagatgac acacgcgcat
     2881 catgatgcag cccaccgcca cgagcggcgc cgtggcgaag ccgaattcct cggcgcctaa
     2941 caacgcagcg atggccacgt cgcggcccgt cttcaactgg ccgtcggtct cgacgacgat
     3001 gcgcgatcgc aggttgttga tgaccagcgt ctggtgcgtt tcggcgaggc cgagttccca
     3061 cggcaggccg gcgtgaatca acgaggtgag cggcgatgcg ccggtgccac cgtcggcgcc
     3121 ggagatcagc acgacgtccg cgtgcgcctt ggcgacgccg gcggcaatgg tgcccacgcc
     3181 cacttcggcg accagcttca cgctgatgcg cgcgtcgcga ttcgcgttct tgagatcgtg
     3241 gatgagctcc gcgagatctt cgatggagta gatatcgtga tgcggcggcg ggctgatgag
     3301 ccccacgccg gccgtggtgc cgcgcgtctt cgcgatccac ggatacacct tggtaccggg
     3361 cagctgtccg ccctcgccgg gcttcgcgcc ctgcgccatc ttgatctgca gttctttcgc
     3421 gttgaccagg tagtggctgg tgacgccgaa gcggcccgac gccacctgct tgatggccga
     3481 gttcttcgag tcgccgttgt ccatcggcac gtagcggtcc ggatcttctc cgccttcgcc
     3541 ggtgttggac ttgcccccca cacggttcat ggcgatggcc agcgtctcgt gcgcttcctt
     3601 ggagatggag ccgtagctca tggcgccggt cttgaagcgc ttcatgaggc tttcgacgga
     3661 ctccacttcc tcgatcggta tcgactcgcc ctgcttgaag tcgagcaggc cgcgcagggt
     3721 a
//
LOCUS       DATDEU010000516         4254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_243994, whole genome shotgun sequence.
ACCESSION   DATDEU010000516 DATDEU010000000
VERSION     DATDEU010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243994"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VIV63_12335"
     CDS             <1..913
                     /locus_tag="VIV63_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6125431.1"
                     /translation="KPSAAEGQTYRGDHAYVFYQVPAKARKLPLILWHGAGQFSKTWE
                     TTPDGREGYQNIFLRRRFSVYVLDQPRRGNAGRSMTEATIKPVPDEQNWFGVFRIGIW
                     PNYFPGVQFSQDPKALDQYFRQMTPNVGPFDLEVVTDAGAKLLEKTGPAILVTHSQAG
                     GPGWVTAIKSSNVRGIVAYEPGSNFVFPEGEVPPAQPSSSGPVEAWAVPAHDFKKLTR
                     IPIVIYYGDNIPKEPDAHPGQDGWRARLAMARLWRDAVNRQGGDVTVVHLPDIGIRGN
                     THFPFSDLNNLQIADLMAKFLANKKLD"
     gene            964..1806
                     /locus_tag="VIV63_12340"
     CDS             964..1806
                     /locus_tag="VIV63_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125432.1"
                     /translation="MVVWLAAALALAFLLLCLPYDPPKWTASEIRKRVDLLNENAAVT
                     TPHLMLGKKTRKENLALFEKVESGQPLTAGESADYRVLYQSILRDKQSELAKIDRQLT
                     VLTNFRPEVNNNVGTLGIEGSHDHHDASAAVNLSALREDLARLDQASGVAASFTRVRA
                     AIRAYKDLTDIIEHMGTAPQTKSVPNEPKAPADELQQHFETMMVHYKRAQFEPVNSPA
                     YVTEVHRALDSYDLLVTLVQDRIYGKLGPLERSLSGRWGSWRSLGPSLSKVSDHRIPR
                     KPGD"
     gene            complement(1812..3905)
                     /locus_tag="VIV63_12345"
     CDS             complement(1812..3905)
                     /locus_tag="VIV63_12345"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY6125433.1"
                     /translation="MARIPLRSIAALLMSLVWGLSHAQDSEPYEPDPPERAARLSYIE
                     GEVSMQPAGEEEWAPALLNRPLTTGDRLWAERDARAEIQVGPASVRLGSNTGFSFLNV
                     NDNTIQMRMTAGVLFVSVRTLGDSEQIEIDTPNVALSLLRAGTYRVEVNDAGDSTVVK
                     VSEGAVQATGPSQDIVVHSQQVVTFDGVDNLVARHATLGAPDEFDSWNLERDRRDDRA
                     ATSRTAEYVSPDVTGYQDLDDNGTWSSEAEYGYVWTPSRVAVGWAPYRYGRWVWVSPW
                     GWSWIDDARWGYAPFHYGRWAHVRNRWCWVPGPRHYRAVYAPALVGWVGAPGVHVSWF
                     PLGPREYYVPGRHYSRHYLERVNFSNTHVNRSHFDRALEHRVRDVVYRNRSVPGGVTV
                     VSRDSFTSARRTGDHRVRVNDQDFSRNVASAVAPRIAPVRESRLGGPARTNVRVPPSS
                     IANRQVVVRRDPAPAAARYARRPTPTTDVAQTLRERTARAPDRRQDRPPRVERPNNEP
                     AAPTAHSSVHDRNAIANVVREDRDRQVRGEQQRREAVQLREGLQQREALQQREDADRQ
                     RWQRQDEQSRQMREQARAVRETQQPDRERPQRQMDAVRQATERQVQQRQQVEQRQQME
                     QRQRSDQRQQMEQRQQAEQRQQSEQRQRSEQRQQPEQRERQQVERQRPEPQRQSEPRS
                     QPRGHEDGNSRSKRD"
     gene            3966..>4254
                     /locus_tag="VIV63_12350"
     CDS             3966..>4254
                     /locus_tag="VIV63_12350"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEY6125434.1"
                     /translation="MRVYDFNSAIVRKPGQSVVNGLRANPGPAPVFETIVAEHQAYID
                     ALRAAGVQVTVLDALEQFPDSIFVEDPALVFSEAAVLLRPGAPSRMAEAREL"
BASE COUNT          731 a         1382 c         1385 g          756 t
ORIGIN      
        1 caagccatcc gccgccgaag gacaaacgta ccggggggat cacgcgtacg tgttctatca
       61 agtgcccgcg aaggcgcgaa agcttccgtt gatcctctgg catggtgccg gccagttctc
      121 gaagacctgg gaaaccacgc ccgatgggcg ggagggctat cagaacattt ttctgcgccg
      181 ccgtttcagc gtgtacgtcc tcgatcagcc gcggcgcggc aatgcgggcc gcagcatgac
      241 cgaggcgacg atcaagccgg ttcccgacga gcaaaactgg ttcggtgtct ttcgcatcgg
      301 catctggccc aactacttcc cgggagttca gttctcgcag gacccgaagg cgctcgacca
      361 atatttccgg cagatgacgc cgaacgtcgg accgttcgac ctcgaagtgg tcaccgacgc
      421 aggcgcgaag ctcctcgaga agaccgggcc ggccattctc gtgacgcatt cacaggccgg
      481 aggcccgggc tgggtcaccg cgatcaagag ttcgaacgtg cgcggcatcg ttgcgtatga
      541 gcccggcagc aacttcgttt ttccggaagg cgaggtaccg ccggcgcagc cgagttcgag
      601 cgggccggtg gaggcgtggg ctgtgcctgc tcatgacttc aagaagctca cgcgcatccc
      661 gatcgtcatc tactacggcg acaacattcc gaaggaaccc gacgcgcatc cgggccagga
      721 cggctggaga gcgagactgg cgatggcgcg actgtggcgc gatgcggtga accgccaggg
      781 tggtgatgtc accgtggtgc atctccccga catcggcatc cgcggaaaca cgcattttcc
      841 attttcggat ctgaacaacc tgcagatcgc cgatctcatg gcgaagttcc tggctaacaa
      901 gaagctggac tgagcgcggg tggataggta ccttaccgtg cagtgaaaac acggttcagg
      961 cgggtggtag tttggctggc ggcggcgctc gcgcttgcat tcttgctgtt gtgcctgccc
     1021 tacgatccgc ccaaatggac agcgagcgag atccgcaagc gcgtcgacct gctcaacgag
     1081 aacgctgcag ttaccacgcc ccatctcatg ctgggcaaga agacgcgaaa ggaaaatctc
     1141 gcgcttttcg agaaggtgga gagcggccag ccactgaccg ccggcgaaag cgcggactat
     1201 cgcgtgctgt atcaatccat cctccgggac aagcagtccg agctcgcgaa gatcgatcgc
     1261 cagctcaccg tgctcaccaa tttccggccc gaggtcaaca acaacgtcgg cacgctcggc
     1321 atcgaagggt cacacgatca tcacgatgcc tcggctgcgg tgaacctctc ggcattgagg
     1381 gaagatctgg cgcggctgga ccaggcgagc ggggtggcag cgtctttcac ccgcgtgcga
     1441 gcggcgatcc gtgcctacaa ggatctcacc gacatcatcg aacacatggg cacggcgccg
     1501 cagaccaagt cagtacccaa cgaacccaag gcgccggccg acgaactgca acagcacttc
     1561 gaaacgatga tggtccacta caagcgtgcg cagttcgagc ccgtcaattc gcccgcctat
     1621 gtgaccgagg ttcatcgggc gctggatagc tacgacctgc tcgtgacgct cgtgcaggac
     1681 cgcatctacg gcaagttagg tccgctggag cgaagcctca gcggacgatg gggaagctgg
     1741 cgttcgctcg gaccctcgtt gagcaaagtg tcggaccacc gcattccgcg gaagccaggc
     1801 gactgacatt ctcagtcccg cttcgagcgg gagttgccgt cctcatggcc gcgaggctgt
     1861 gatcgcggtt cgctttgccg ttgcggctcg ggacgctgcc gctccacctg ctggcgctcg
     1921 cgttgttcgg gctgctgacg ttgctccgat cgctggcgct gttccgactg ctggcgctgc
     1981 tccgcctgtt ggcgttgttc catctgctgg cgctggtcgg agcgttggcg ctgctccatt
     2041 tgctgacgct gttccacctg ctgacgctgc tgcacctggc gctcggtggc ttgtcgcacg
     2101 gcgtccatct ggcgctgagg ccgctcacgg tccggctgct gcgtttcgcg tacggcacgg
     2161 gcctgctcac gcatttgccg ggactgctca tcctgccgct gccaacgctg ccgatccgca
     2221 tcctcgcgct gttgcaacgc ttcgcgctgc tgcaggcctt cgcgaagctg cacggcctcg
     2281 cgccgctgct gttcaccgcg aacctgccgg tcgcgatctt cgcgcaccac gttcgcgatc
     2341 gcgttccggt cgtgtaccga cgaatgcgcg gtgggcgcag caggctcatt gttgggccgt
     2401 tcgacgcgcg gcgggcgatc ctgcctgcga tcgggtgcac gcgccgtgcg ttcgcgcagt
     2461 gtctgagcaa cgtccgtcgt gggtgtcggg cggcgcgcgt aacgcgcggc ggcaggcgcc
     2521 ggatcccggc gcacgacgac ctggcgattc gcgatggacg aaggcggcac gcgcacgttc
     2581 gttcgcgccg gtccgcccag gcggctctcg cgcacgggcg cgatccgcgg tgccacggcg
     2641 ctcgcgacat tgcgcgagaa atcctgatcg ttgacccgca cgcggtgatc gcctgttcgg
     2701 cgcgccgagg tgaacgagtc gcgtgacacc accgtcacgc cgcccggcac gctgcggttg
     2761 cgatagacga catcgcggac gcgatgttcg agcgcgcggt cgaagtgcga gcgattcaca
     2821 tgcgtgttcg agaagttcac gcgctcgagg taatgccggc tgtagtggcg gccgggcaca
     2881 tagtattcac gcgggccgag cgggaaccac gaaacgtgga ctccaggcgc acccacccaa
     2941 ccgacgagcg ccggcgcgta caccgcgcgg taatgacgtg ggcctggcac ccagcaccag
     3001 cgattgcgca cgtgtgccca tcggccgtag tggaaaggtg cgtaacccca gcgagcgtcg
     3061 tcgatccagc tccagcccca cggcgatacc cacacccagc ggccgtagcg atagggcgcc
     3121 cagcccacgg cgacgcgcga cggcgtccac acgtaaccgt attcggcctc ggagctccac
     3181 gtgccgttgt cgtcgagatc ctggtagccg gtgacgtcgg gcgacacgta ttcagccgtt
     3241 cgcgacgtcg cggcgcgatc gtcgcgccgg tcgcgttcga ggttccacga atcgaattcg
     3301 tcgggcgcgc ccagcgttgc gtgtcgcgcg accaggttgt cgacgccgtc gaaagtaacg
     3361 acctgctgcg agtggaccac gatgtcctgt gaaggaccgg tggcctggac cgcgccttcg
     3421 ctgaccttga cgacggtgct gtcgccggcg tcattcactt cgacgcgata ggtgcccgcg
     3481 cgcagcagcg acagcgcgac gttcggcgtg tcgatctcga tctgctcgct gtcgccgagc
     3541 gtgcgcacgc tgacgaagag cacgcccgcc gtcatgcgca tctggatcgt gttgtcgttg
     3601 acgttgagaa acgaaaagcc ggtattgctt ccaagccgta ccgacgccgg tccgacctgg
     3661 atctcggcgc gcgcatcgcg ctcggcccag agcctgtcgc cggtggtcag cggacggttg
     3721 aggagggccg gcgcccattc ttcttcgccg gccggttgca tcgacacttc gccttcgatg
     3781 taactcaggc gcgcggcgcg ctccggcggg tccggttcgt aaggctcgct atcctgtgcg
     3841 tgggaaagcc cccacacgag tgacataagc agagccgcga tcgagcgcaa tggaatacgt
     3901 gccatggtac gcatcataga ggctcgctct gaacgctccc tgaacggata agctccagca
     3961 aaatcatgcg cgtgtacgac ttcaactccg ccatcgtgcg caagcccggc cagagcgtgg
     4021 tcaacggcct gcgcgcgaat cccgggccgg cgccggtgtt cgaaaccatc gtcgccgagc
     4081 accaggcgta catcgacgcg ttgcgggcgg ccggcgttca ggtcaccgtt ctcgacgcgc
     4141 tggagcagtt tcccgattcg atcttcgtcg aggaccccgc gctggtattc agcgaggcgg
     4201 cagtgttgct gcggcccggc gcgccctcac gcatggcgga ggcacgggaa ttgg
//
LOCUS       DATDEU010000517         6724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1412482, whole genome shotgun sequence.
ACCESSION   DATDEU010000517 DATDEU010000000
VERSION     DATDEU010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412482"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2280
                     /locus_tag="VIV63_12355"
     CDS             <1..2280
                     /locus_tag="VIV63_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY6125435.1"
                     /translation="NGDGSLSDHVDIINMSLGSPYGQPFDDDLSAAVDNASAAGVLTV
                     ASAGNSADKQFITGSPGAATSALSVAQTAVPSATLQLMTLLAPVTGDRGAVFQPWAAP
                     LTTTIEGPVFYPPVTGGKRIGCADAAGTNPYAAGELAGRIVLVDRGTCSFSLKIANVA
                     AAGGILGIIGLITPEAPFGGAFGGGVQTIPAYMINQVDANILRTNTAVVRFSPSGNLS
                     LAGSLASTSSRGPRFDDNIVKPEIGAPGASVSAMSGSFTGTAAFGGTSGAAPMVTGAA
                     AIIKAARPGLSIAEIKQLLVNTANQAVYQPSAGGSVFPDQLAPITRIGGGEVRVDKAL
                     LSPSIVFDVTGDEASQAHGAVSFGLVDVANPSILKTRKLRVVNKSGRAQRYTVTPTQR
                     FQDDVDTGAVVMSWSPASVTVPPGGSANVTLWLTIKGDKLRNNLMSAGSGGNAIGPLT
                     ANEYDGYVNFVARDHTLSIPWHVLPRKSSLVGANKAAQAGLVFDETTGLATAELTNYG
                     IGNAQLQYFTLLGTSPDRPSGGRGEGLPNPDLRAVGVNTLLVPGSTCGLATGNAFIWE
                     FAFNTWERKASPVGTWLEVDLDVNGDGVFDYALLNQDNAGITQLSDGRQVSALLRLSP
                     TGSILSTTVRFFAENSTNTGNTILRACASDMGLTTATVGQPVTAEFFATSWYFGGSSD
                     FVGPFVITPYGEEFTATLPGDELTYQQTGALTVTQWGLFPGTSPGAGLMVVTNSDFGS
                     TSRGGSTPETETLLLPVAP"
     gene            2393..3295
                     /locus_tag="VIV63_12360"
     CDS             2393..3295
                     /locus_tag="VIV63_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY6125436.1"
                     /translation="MVVRHPAGTRRRFLKALAAVGGAALAGARRADAGVRPLFSAIGI
                     TAKVERAAALKAAGADFLVESAADFLVPFAGDAAFAANRARAAASELPIRGCNSFMRD
                     PSLVCVGPRADHVRVIAYAATAFARLQAIGGEYIVFGSNTARRIPDGWSKAQADTQFI
                     ELLKRMAPLAERHGITVGVEMQRTSECNYLNHIEEVVGVVAPVNHANIRVLADLFHMR
                     VMGDSPQQLERAMPWVGLVELAEREQRTLPGIAGDDFRPYFAALSRGQYRGLVDIEGD
                     GTPEELRNAFATVKIQARDAEMRE"
     gene            complement(3305..3838)
                     /locus_tag="VIV63_12365"
     CDS             complement(3305..3838)
                     /locus_tag="VIV63_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125437.1"
                     /translation="MSKLLSGFLVFGVVMAIALPPPVYAGDKPATPRNIAPPTRIPDP
                     VVAEAPGEHVDIASVPRAVRRAVVADAARRFAVSENEVVLINAESVTWSDGSMGCPQP
                     GQMYTQMLVPGFRLAAKTSAGQMVYHTDSRGTVVNCAAGKFQTGPKQLSAHPAHDGTG
                     TGAEPRTQPPQPKAPDR"
     gene            3894..4712
                     /gene="rlmJ"
                     /locus_tag="VIV63_12370"
     CDS             3894..4712
                     /gene="rlmJ"
                     /locus_tag="VIV63_12370"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY6125438.1"
                     /translation="MKYRHQYHAGNFADVHKHVALLEVLHALTRKDKGLLFVDTHAGR
                     GEYDLHPGDREHPAEWQAGVARLLAATPRHPAIVRYRDIVARGLHGGSLRYAGSPLIA
                     TQLLRPVDRAVFFETQREEAGHLRKVLEGVDRTRIEGADGFAGLRGLMPPPERRGCVL
                     IDPPYEEREDLARAVAAAKDALTRFESAVILLWLPVKLRTDFDMWFKSVGSAITRPLL
                     ASLLWMHPCDSRAALNGSALVLINPPYLVEEGMREWLPELRELLGGPQSGCEVL"
     gene            complement(4714..5382)
                     /locus_tag="VIV63_12375"
     CDS             complement(4714..5382)
                     /locus_tag="VIV63_12375"
                     /inference="COORDINATES: protein motif:HMM:NF023287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HEY6125439.1"
                     /translation="MSNEFSCRHARLEIGGDPHGQSPELEAHLATCAACTRFRTETLA
                     LDARLRAAFELPLAQFRKPPAKAAPPARRFALAASVVLALLVGGGAWLFRPSSALAAE
                     IVEHVEHEPGSWQGHDPVSPEVLAAVLARAGVRYDSRLPVTYASPCPFRGHIVPHLVV
                     QTDRGPLTVMVLDHVKSDAEGKFSEGEYRGIVMPAGSGSIAVVARKGQEFDGALKDVL
                     EGVR"
     gene            complement(5379..5903)
                     /locus_tag="VIV63_12380"
     CDS             complement(5379..5903)
                     /locus_tag="VIV63_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6125440.1"
                     /translation="MNPRPRGTTVSSAFTRLLETLRPDLVRFAFWLARDRAVAEDVVQ
                     ETLIRAWKSREELKDSAAARPWLLTIVRREHARLYERKRLPTVDVDQVEALGDPALAV
                     DGDAGLEDLRRAIMQLPDEYREPLVLQVLGGLSTNEIAAELKLTQAAVLTRLFRARNR
                     LRTIYGLEPAEDAE"
     gene            5967..>6724
                     /gene="aroE"
                     /locus_tag="VIV63_12385"
     CDS             5967..>6724
                     /gene="aroE"
                     /locus_tag="VIV63_12385"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY6125441.1"
                     /translation="MNVPDQYGVVGHPISHSWSPFIHGLFAKQTEQSLVYRLYDIAPG
                     DFRAQVLEFFTRGGRGLNVTVPHKEAAAELANELTVRADRAGAVNTLMINAEHSLLGD
                     NTDGHGLVTDLRKNLGLTITNERILILGAGGATRGVVEPLLELGPTELVIANRSPDRA
                     VNLASLFSDLGAIRGCGFEDVGDEPFDIVINATAAGLSGSVPNVDGAIIASHTACYDM
                     SYSKASTPFVSWAIEQGCARAHKGWGMLVEQAAEA"
BASE COUNT         1127 a         2279 c         2158 g         1160 t
ORIGIN      
        1 aatggcgacg gcagcctcag cgatcacgtc gacatcatca acatgtcgct cggttcgccg
       61 tacggtcagc cgttcgatga cgacctctcc gccgcggtgg acaacgcctc ggctgcgggt
      121 gtcctcacgg tggcgtccgc cggcaacagt gccgacaagc agttcatcac cggttcgccc
      181 ggtgctgcga cgtcggccct gagcgtcgcg cagaccgcgg ttccgtcggc cacgctgcag
      241 ctcatgacgc tgctcgcgcc ggtcacgggt gatcgcggcg cggtgttcca gccgtgggcg
      301 gctccgctaa ctaccaccat cgaaggcccg gtgttctatc cgccggtcac cggcggcaag
      361 cgcatcggtt gcgccgacgc ggccggcacc aacccgtacg cagctggcga actcgccggc
      421 cgcatcgtgc tggtcgatcg tggcacctgc tcgttcagtc tcaagatcgc caacgtggcg
      481 gcggcgggcg gaatactcgg catcatcggc ctcatcactc cggaagcgcc cttcggtggc
      541 gcgttcggcg gtggcgtgca gacgattccg gcgtacatga tcaaccaggt ggacgcgaac
      601 atccttcgca ccaacaccgc ggtcgtgcgt ttctcgccga gcggcaatct gtcgctggcg
      661 ggttcgctgg cctcgacctc ttcgcgcggt ccgcgcttcg acgacaacat cgtcaagccg
      721 gaaattggcg cgccgggtgc atcggtgtcg gccatgtcgg gcagcttcac gggcaccgcc
      781 gcgttcggcg gcacctcggg agccgctccc atggtcacgg gcgccgcggc catcatcaag
      841 gccgctcgtc cgggtctgtc gatcgccgag atcaagcagc tgctggtgaa tacggcgaat
      901 caggccgtgt atcagccatc ggctggcggc tcggtgttcc cggatcagct cgcgccgatc
      961 acgcgcatcg gtggtggtga agtacgtgtc gacaaggcac tgctctcgcc gtccatcgtg
     1021 ttcgacgtga cgggtgacga agcctcgcag gctcatggtg cggtcagctt cggtctcgtc
     1081 gatgtggcga atccgagcat cctcaagacg cgcaaactgc gcgtcgtcaa caagtccggc
     1141 cgcgctcagc gttacaccgt aacgccgaca cagcgcttcc aggacgacgt cgacacgggt
     1201 gcggtggtca tgagctggag tcccgcttcg gtaaccgtac cgccgggtgg ttcggccaac
     1261 gtcacgttgt ggctcacgat caagggcgac aagctgcgca acaacctcat gagcgcaggc
     1321 tcgggtggta atgcgatcgg acccttgacg gccaacgaat acgacggcta cgtcaacttc
     1381 gttgcccgcg atcacacgct gtccattccc tggcacgtgc tgccgcgcaa gtcctcgctc
     1441 gtaggggcta acaaggccgc gcaggctggt ctcgtgttcg acgagaccac gggcctcgcg
     1501 accgccgagc tcaccaacta cggcatcggc aacgcgcagc tgcagtactt cacgctgctc
     1561 ggcacgagcc cggatcgtcc gtcgggcggt cgtggcgaag gcctgccgaa ccccgatctg
     1621 cgcgctgtcg gcgtgaacac gctgttggtt ccggggtcga cttgcggcct ggctaccggc
     1681 aatgcgttca tctgggagtt cgcgttcaac acgtgggaac gcaaggcgtc gcctgtgggc
     1741 acgtggctcg aagtcgacct cgacgtcaac ggtgacggcg tattcgacta cgcgctgctc
     1801 aaccaggaca acgctggcat cacgcagctc tccgacggcc gccaggtatc agctctgctg
     1861 cgcctgtcgc cgaccggttc gatcctgtcg accacggtcc gcttcttcgc ggaaaactcc
     1921 accaacacgg gcaacacgat cctgcgtgcc tgcgccagcg acatgggcct gacgaccgcc
     1981 acggtcggtc agccggtcac ggcggagttc ttcgccacgt cgtggtactt cggtggttcg
     2041 tccgacttcg tcggaccgtt cgtcatcact ccgtacggtg aagagttcac cgccacgctg
     2101 ccgggtgacg agctgaccta ccagcagacc ggcgccctga cggtcacgca gtggggtctg
     2161 ttcccgggca cgagccctgg cgcgggcctg atggtcgtga ccaactccga cttcgggtcc
     2221 accagtcgcg gtggctcgac gccggaaacg gaaacgctgt tgcttccagt cgctccctga
     2281 tccggacgac gagtagacag agggcccccg cgggaaaccg cgggggcctg ttcttttgag
     2341 gcggccatcg gagcgttgac gcgccccggc cgcggtgctc caatccggcg ccatggtagt
     2401 tagacacccg gccggcacgc gacgccgatt tctcaaggcc ctcgcggccg tgggaggagc
     2461 cgcgctggca ggcgcgcgac gggccgacgc cggtgtgcga cccctgttca gcgccatcgg
     2521 catcaccgcc aaggtggaac gagccgccgc gctcaaggcc gctggcgccg atttcctggt
     2581 cgaaagcgcc gcggacttcc tcgtcccgtt cgcgggtgac gccgcatttg ccgcgaaccg
     2641 cgcccgtgcg gccgcatccg aattgcccat ccgcggctgc aacagtttca tgcgcgatcc
     2701 ctcgctggta tgcgtggggc cacgtgccga ccatgttcgc gtgatcgcct acgcggcgac
     2761 ggcctttgcc aggctgcaag ccatcggcgg tgaatacatc gttttcggca gcaatacggc
     2821 gcggcgcatt cccgatggct ggagcaaggc gcaggccgat acgcagttca tcgagttgct
     2881 gaagcgtatg gcgccgctcg ccgagcgcca cggcatcacc gtcggtgtcg agatgcagcg
     2941 caccagcgaa tgcaactatc tcaatcacat cgaagaagta gttggcgtgg tcgcgccggt
     3001 gaaccacgcg aacatccgcg tgctcgccga cctctttcac atgcgtgtga tgggcgattc
     3061 gccgcagcag ctggagcgcg ccatgccgtg ggtaggactt gtggaactcg ccgaacgcga
     3121 gcaacgcacg ttgcccggca tcgccggtga cgacttcagg ccttacttcg ccgcactttc
     3181 acgcggccag taccgcgggc tcgtggacat cgaaggtgat ggcacgcccg aagaactgcg
     3241 caacgcattc gcgaccgtga aaatccaggc gcgcgacgcg gaaatgcgtg agtgatccga
     3301 aggatcagcg gtccggagcc ttgggctgag gtggctgggt gcgcggctcg gcgcccgtcc
     3361 cggtgccgtc gtgagcggga tgcgcggaca gctgttttgg tccggtctgg aattttccgg
     3421 cggcgcagtt cacgaccgtg ccgcgcgaat cggtgtgata caccatctgc ccggcgctgg
     3481 ttttcgccgc gagtcgaaac cccggcacca gcatctgcgt atacatctgc ccgggctggg
     3541 gacaacccat cgagccatcg ctccaggtca cactttcggc gttgatcagc accacctcgt
     3601 tttcgctcac cgcgaaccgc ctggcggcgt cggccaccac ggcccgccgc acggcgcgtg
     3661 gcacgctcgc gatatccacg tgttcacccg gcgcctccgc gacgacggga tcgggaattc
     3721 gagtgggcgg tgcgatgttc cgcggtgtcg ccggcttgtc gcccgcgtac accggcggcg
     3781 gcagcgcaat ggccatgacg acaccgaaaa ccaggaaccc ggacagcagc ttcgacatca
     3841 atttctccgt gtcagacagt gtataaacgc gggcggcgca ctgccggggt tccatgaaat
     3901 atcgccacca atatcatgcc ggcaacttcg ctgacgtcca caagcacgtg gccctgctcg
     3961 aggtcctgca tgcgctcacg cgcaaggaca agggcctgct gttcgtcgac acacacgcgg
     4021 gccgcggcga atatgacctg catcccggcg accgcgagca tcccgcagaa tggcaggcgg
     4081 gcgtcgcacg gctgctggcg gccacgccgc ggcatccggc aatcgttcgt tatcgcgaca
     4141 tcgtcgcgcg cggcctgcat ggtggcagcc tgcgttacgc gggatcgccg ctgatcgcca
     4201 cccagttgct gcgccccgtg gatcgcgcgg tgttcttcga aacccaacgg gaagaagcgg
     4261 gccatctgcg caaggtgctg gaaggagtcg accgtactcg catcgaaggc gccgatggct
     4321 tcgccggtct gcgcgggctg atgcccccac ccgagcggcg cggctgcgtg ctcatcgatc
     4381 cgccttacga agaacgcgaa gacctggcgc gcgccgttgc cgctgccaaa gacgcactga
     4441 cgagattcga gagcgcggtg atcctgctgt ggctgccggt gaagctgcgc accgacttcg
     4501 acatgtggtt caagagcgtg ggctccgcta tcacgcggcc gctcctggcg tcattgttgt
     4561 ggatgcatcc gtgcgattcg cgcgcggcgc tcaacggatc ggccctggtg ctcatcaacc
     4621 cgccctatct agtcgaagag ggcatgcgcg aatggctgcc ggaacttcgc gagttgcttg
     4681 gcggaccgca gtccggctgc gaagtcctgt agttcagcgc accccttcga gcacgtcctt
     4741 cagggcgccg tcgaattcct gacccttgcg agcgaccacg gcgatgctgc cggaacccgc
     4801 cggcatcacg attccgcgat actccccttc gctgaacttt ccttccgcgt cgcttttcac
     4861 gtgatcgagc accatcacgg tgagcggtcc gcgatcggtc tgcacgacga ggtgcggcac
     4921 gatgtggccg cgaaacggac agggcgacgc ataggtcacc ggcaggcggc tgtcgtaacg
     4981 cactcccgcc ctggcgagca cggcagcgag gacttcgggc gacaccggat catgaccctg
     5041 ccaactaccc ggctcgtgct ccacatgttc gacgatctcc gctgccagcg ccgacgaagg
     5101 ccggaacagc cacgcaccgc cacccaccag cagggccagc accaccgacg ccgcgagcgc
     5161 gaagcggcgc gccggaggcg cggctttcgc gggcggcttg cgaaactgcg ccaggggcaa
     5221 ttcgaaagcg gcgcgcaacc gcgcgtccag cgcgagggtc tcggttcgaa accgcgtgca
     5281 ggcggcgcag gtagcgagat gggcttcgag ttcgggcgac tgcccgtgcg ggtcgccgcc
     5341 tatctccagg cgcgcgtgtc tgcagctgaa ttcgttgctc attccgcgtc ctccgcgggt
     5401 tcgaggccgt agatagtgcg cagccggttg cgcgcgcgga acagccgcgt cagcaccgcc
     5461 gcctgggtga gcttcagctc cgcggcaatt tcgttcgtgc tcaaaccgcc taacacctgc
     5521 aacaccaatg gctcccgata ctcgtccggc aactgcatga tggcgcgccg caggtcttcc
     5581 agcccggcgt cgccgtcgac ggccagggcc ggatcgccca gtgcctcgac ctgatccacg
     5641 tccaccgtcg gcagtcgctt gcgctcgtac aggcgcgcat gctcgcgacg cacgatggtg
     5701 agcagccagg ggcgcgcggc cgccgaatcc ttgagctcct cgcgggattt ccaggccctg
     5761 atgagtgtct cctgcaccac gtcctccgcc acggcccggt cgcgcgccag ccagaaggca
     5821 aagcgcacca ggtccgggcg caaggtctcc agcaatcgcg tgaacgcgga ggacaccgtc
     5881 gttccgcggg ggcgtgggtt catgtataag accggccgag tgaatagctt atttcagcca
     5941 ttttcctgtt cattgatgga cgggcaatga acgtaccgga tcagtacgga gtggtcggtc
     6001 atccgatcag tcacagctgg tcgcctttca tccacgggct ctttgcgaaa cagaccgagc
     6061 agtccctggt ctatcgtctt tatgacattg ccccggggga cttccgcgcg caggtgctgg
     6121 aattcttcac acgcggcggc cgtgggctga acgtgaccgt accacacaag gaagccgcgg
     6181 ccgagcttgc caacgagctc acggttcgcg ccgaccgggc cggggccgtg aacacgctca
     6241 tgatcaacgc ggaacacagc ctgctcggcg acaacaccga cggccacggg ctggtcaccg
     6301 acctgcgcaa gaacctcggc ctgacgatca ccaacgagcg catcctgatt ctcggtgccg
     6361 gcggcgcaac tcgcggcgtg gtcgagccgc tgctcgaact cggccccacc gaactggtga
     6421 tcgccaaccg ctcgccggac cgcgcggtca acctggcctc gctgttctcc gatctcggtg
     6481 cgatccgtgg ctgcggtttc gaagacgtgg gcgacgagcc gttcgacatc gtcatcaacg
     6541 ccactgccgc cgggctttcg ggatccgtac ccaacgtgga tggcgccatc atcgccagcc
     6601 ataccgcctg ttacgacatg agctacagca aggcatccac tcctttcgtg agctgggcca
     6661 ttgaacaggg ctgcgcgcga gcccataaag gctggggcat gctcgtcgag caggccgctg
     6721 aagc
//
LOCUS       DATDEU010000518         4678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_539228, whole genome shotgun sequence.
ACCESSION   DATDEU010000518 DATDEU010000000
VERSION     DATDEU010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539228"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3099
                     /locus_tag="VIV63_12390"
     CDS             <1..3099
                     /locus_tag="VIV63_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY6125442.1"
                     /translation="TMDDTYKRVARALADVEHETVREVWYEKFVWALRRGAIPAGRVT
                     SNAGALEHKPATSTINCTVSGTIGDSMDDILNKVHEAGLTLKAGCGIGYEFSTLRPRG
                     AYVSGAGAYTSGPLSFMDIYDKMCFTVSSAGGRRGAQMGTFDVGHPDAMEFIRAKREN
                     GRLRQFNLSLLVTDDFMKAVRADTDWNLAFPLLTKEYDPAEHKLDDTTKYLWREWPTT
                     RNYVTREDGLVCCKIYKTLPARRMWDVIMTSTYDFAEPGFILIDRVNEMNNNWWCENI
                     RATNPCVPADTWVQTSEGPRQVAELIGKQFSARVDSVDHASSPEGFFRTGVKPLVSVT
                     TSEGHSLRLTSDHRVRRISTLTRFRLVTEWCAAGDLAAGDRVLLQDHRRAASWTGETG
                     VKELAANEGYLMGLLVGDGTVSEDRAELSIWARPAVGNSDIDVGGPHSVMAAALDAAR
                     CFNHRADFSGWHPGTGRGEFRLAMGAITELSREVGLRPGRKTITPAIERASSEFYRGF
                     LRGLFDTNGSVQGNQMKGVSVRLANSDLTQLEGAQRMLLRLGIAASMYERAQHELVIT
                     GENVARFAEVVGFADTVKRARLSRLLGGYKRTLNRERFVATVESVRADGMEDVYDVQI
                     PGINEFDANGFHAHNCGEQPLPPYGSCLLGSVNLTRFVKNAFTDFAEFDWNEYREVVK
                     VFTRMLDNVCEINGLPLEQQRMEIMRKRRHGMGFLGLGSTITCLGLKYGSEESVKFTE
                     DVSREMAVAGWEAALDLAKEKGPAPIMKEEFSVTAEMLRKRPEMAKDGWKIGDLIPGR
                     ILHARYSKYMQRVAETAPWLVDQLANVGARFTHHTSIAPTGTISLSLANNASNGIEPS
                     FAHHYFRNVIRPGKKSKEKVDVFSFELLAYRELINPRAMPNSTVPAEQLPGYFTTADD
                     ITPREHVDIQAAAQRWVDSSISKTANVPTDYKYESFKDIYLYAHEKDLKGCTTFRFNP
                     EAFQGVLVKEDDLKNTTYVFTLEDGSEISAKGDEEIEYDGEKHTAANLYDALKEGYYG
                     KF"
     gene            3113..3898
                     /locus_tag="VIV63_12395"
     CDS             3113..3898
                     /locus_tag="VIV63_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125443.1"
                     /translation="MHKIDKKIAKYRVQKAGDAAAEKAAANAAEAATAATVATAVAAA
                     VAKFEPNVEMTHDKQGRSSKVVRMTEEVQRPEVLVGSTYKIKTPVSDHAMYVTINDII
                     LNEGTPYEQRRPFEVFINSKNLDHFQWIVALTRIISAVFRKGGDVTFLVDELKAVFDP
                     RGGYWQAGGKFMPSIIAELGYVIEKHLQTIGLLKKVELDDHEKKLIAEKKAEFEERTK
                     QTDAFAKSHFPEGAQLCAKCSTAAVVMMDGCMTCLNCGDSKCG"
     gene            4173..4562
                     /locus_tag="VIV63_12400"
     CDS             4173..4562
                     /locus_tag="VIV63_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125444.1"
                     /translation="MKLAAIATAVVSSLMLAAMAFADASPTSNKWRIEMSGQALTTGD
                     IVFRVTPRQGEAVDVSVGIKSGRDENNVAKDVRDALAAKLSPDRYSIEVDDGEDILIK
                     KKEGQPDFALELVESNVQNVSIKVEGE"
BASE COUNT          957 a         1496 c         1440 g          785 t
ORIGIN      
        1 accatggacg acacctacaa gcgcgtcgca cgcgccttgg cggatgtcga acatgaaacc
       61 gttcgcgagg tctggtacga gaaattcgtc tgggctttgc gccgtggcgc catcccggcc
      121 ggtcgcgtga cgtccaatgc gggcgctctc gagcacaagc ccgccacctc gaccatcaac
      181 tgcacggtgt cgggaaccat cggcgattcg atggacgaca tcctgaacaa ggtgcatgag
      241 gccggcctca cgctgaaggc gggctgcggc atcggttacg aattctcgac gctgcgtccg
      301 cgcggcgcat acgtctcggg cgcgggtgcc tacaccagcg gtccgctgtc gttcatggat
      361 atctacgaca agatgtgttt caccgtgtcg tcggccggcg gccgccgcgg cgcgcagatg
      421 ggcactttcg acgtcggcca tccggacgcc atggaattca tccgcgccaa gcgcgagaac
      481 ggccgcctgc gccagttcaa cctgtcgctg ctggtgaccg acgatttcat gaaagcggtc
      541 cgcgccgaca cggactggaa cctggccttt ccgctgctca ccaaagagta cgacccggcc
      601 gaacacaaac tcgacgacac cacgaaatac ctgtggcgcg agtggccgac cacgcgcaac
      661 tacgtcaccc gtgaagacgg gctggtgtgc tgcaagatct acaagacgct gccggcgcgc
      721 cgcatgtggg acgtcatcat gacctccacc tacgacttcg ccgagccggg cttcatcctc
      781 atcgaccgcg tcaatgagat gaacaacaac tggtggtgcg aaaacatccg cgccaccaac
      841 ccctgcgttc ccgccgatac ctgggttcag accagcgaag gtccgcgcca ggtcgccgag
      901 ctcatcggca agcagttcag tgcgcgcgtc gacagcgtcg atcacgccag ttcgccggaa
      961 ggtttcttcc gtacgggcgt aaagccgctg gtaagcgtga cgacgtccga aggccattcg
     1021 ctgcgcctga cgtccgacca tcgcgtgcgc cgcatttcca cgctgacgcg tttccgcctg
     1081 gtaaccgagt ggtgcgccgc gggcgacctc gccgcgggcg accgcgtgct cctccaggat
     1141 catcgtcgtg ccgcatcctg gaccggtgaa accggtgtga aagaactggc ggccaacgag
     1201 ggctacctca tgggcctgct cgtcggcgac ggaaccgtgt ccgaagaccg cgccgaactg
     1261 tcgatctggg cgcgtcccgc ggtcggtaac agcgacatcg atgtgggtgg tccccattcc
     1321 gtcatggccg ccgcgctcga tgccgcgcgt tgcttcaacc accgcgccga cttctccggc
     1381 tggcaccccg gcaccggccg cggcgagttc cgcctggcaa tgggcgcgat caccgaactg
     1441 tcgcgtgaag tggggctgcg gcccggccgc aagaccatca cgcccgccat cgaacgcgct
     1501 tcgagcgagt tctatcgcgg tttcctgcgc ggactgttcg acacgaacgg cagcgtgcag
     1561 ggcaaccaga tgaagggtgt gtcggttcgt ctcgccaatt ccgacctcac ccagctcgaa
     1621 ggcgcgcagc gcatgctgct gcgtctcggc atcgccgcca gcatgtacga gcgcgcgcag
     1681 cacgaactcg tcatcaccgg cgagaacgtc gcccgattcg cggaagtcgt ggggttcgcc
     1741 gacacggtca agcgcgcgcg tctgtcgcgc ctgctgggcg gctacaagcg cacgctcaat
     1801 cgcgagcgct tcgtggccac cgtcgagagc gtgcgtgccg acggcatgga agatgtgtac
     1861 gacgtgcaga tccccggcat caacgagttc gatgccaacg gtttccacgc gcacaactgc
     1921 ggcgaacagc cgctgccgcc gtatggttcg tgcctgctcg gctcggtgaa cctcacgcga
     1981 ttcgtgaaga acgcgttcac cgatttcgcc gagttcgact ggaacgaata ccgcgaagtc
     2041 gtgaaagtgt tcacgcgcat gctcgacaac gtctgcgaga tcaacggact gccgctcgag
     2101 cagcagcgca tggagatcat gcgcaagcgt cgtcacggca tggggttcct cggcctcggc
     2161 agcaccatca cgtgtctcgg cctcaaatac ggctcggaag agtcggtgaa gttcaccgaa
     2221 gacgtgtcgc gtgaaatggc cgtggccggc tgggaagccg cgctcgatct cgcgaaagag
     2281 aagggcccgg cgcccatcat gaaagaagag ttcagcgtca cggccgagat gctgcgcaag
     2341 cgtccggaaa tggcgaagga cggatggaag atcggcgacc tcatccccgg ccgcatcctg
     2401 cacgcgcgct acagcaagta catgcagcgc gtggcggaaa cggcgccgtg gctcgtggac
     2461 cagctcgcca atgtcggcgc gcgattcacg caccacacgt ccatcgcgcc caccggcacc
     2521 atctcgctgt cactggccaa caacgccagc aacggcatcg agccgtcgtt cgcacatcac
     2581 tatttccgca acgtcatccg gcccggcaag aagagcaagg agaaagttga tgtcttctca
     2641 ttcgagcttc tcgcctatcg cgagctcatc aatccgcgtg cgatgccgaa ctcaacggtc
     2701 ccggccgaac agttgcctgg ctatttcacg acggcagatg acatcacgcc gcgagagcac
     2761 gtcgacatcc aggcggcggc gcaaaggtgg gtcgacagct ccatttccaa gactgcgaac
     2821 gtgcctacgg attacaaata cgagagcttc aaagacatct atctgtacgc ccatgagaag
     2881 gatctgaagg gctgcacgac gttccgcttc aatccggaag ctttccaggg cgtgctggtg
     2941 aaggaagacg acctcaagaa cacgacctac gtgttcacgc tcgaggacgg atcggagatc
     3001 tcggcgaagg gtgacgaaga gatcgaatac gacggtgaaa aacacaccgc cgcgaatctc
     3061 tacgacgcat tgaaagaagg ctattacggc aagttctagc cggagacgac tcatgcacaa
     3121 gatcgacaag aaaatcgcga agtaccgcgt gcagaaggcc ggggacgcgg ccgctgaaaa
     3181 agcggctgca aacgctgccg aagccgccac cgccgccacg gtggccacgg cggttgccgc
     3241 cgcggtcgcc aagttcgagc ccaacgtcga gatgacgcac gacaagcagg ggcgttcttc
     3301 caaggtcgtg cgcatgaccg aggaagtgca gcgccccgag gtgctggtcg gctccacgta
     3361 caagatcaag acgccggtgt ccgatcacgc catgtacgtg acgatcaacg acatcatcct
     3421 caacgaaggg acgccgtacg aacagcgccg cccgttcgag gtgttcatca actcgaagaa
     3481 cctcgatcac ttccagtgga tcgtggcgct cacgcgcatc atctccgccg tgttccgcaa
     3541 gggcggcgac gtcacgttcc tggtcgatga attgaaagcc gtgttcgatc cgcgcggggg
     3601 atactggcag gcgggcggca agttcatgcc gtcgatcatc gccgagctgg gctacgtcat
     3661 cgagaagcac ttgcagacca tcgggctgct caagaaggtg gaactcgatg accatgagaa
     3721 gaagctgatc gccgagaaga aggccgagtt cgaagaacgc accaagcaga cggacgcgtt
     3781 cgccaagtcg cattttccgg aaggcgcgca gctttgcgcc aagtgcagca ccgccgccgt
     3841 cgtcatgatg gacggctgca tgacctgcct gaactgcggc gattcgaagt gcggctgatc
     3901 gccggaagtt agagagtcat tacgcgtctc gcggccgggc ttcgagcccg gctcccaccc
     3961 tgacgggaaa ggcgattcgc gttttcacaa ggcagtcgaa gggctgcctt gtttttgcag
     4021 cgcccccctg tgtcggtcga cacctgcgtc cctgaaggcg agcgggaagg ccgtcatgcc
     4081 gtatcaccga cattcgcttg cgtagctgtc ggatgccccg gttttgtgtg gcggcggaca
     4141 ctgtcgccac agacagtgac ccggggtatt ccatgaaact tgccgcaatc gcaaccgccg
     4201 tcgtttcctc gctgatgctc gccgccatgg cgttcgccga tgcgtcgccc acgtcgaaca
     4261 agtggcgtat cgagatgagc ggccaggcgc tgactaccgg agacatcgtc ttccgcgtca
     4321 cgccgcgcca gggtgaggcc gtcgacgtga gcgtcggcat caagagcggc cgcgatgaga
     4381 acaatgtggc caaggacgtg cgcgacgcgc tcgcggccaa actcagcccc gaccgttaca
     4441 gcatcgaagt cgacgacggt gaggacatcc tcatcaagaa gaaggaaggc cagccggact
     4501 tcgcgctcga gctcgtcgaa tcgaatgtgc agaacgtcag catcaaggtc gaaggcgaat
     4561 aaccttcgca aaaacgtccg ggcgccccgg aattcgccgg ggcgcgccgg cccgatttgc
     4621 cggagccgaa ccatgaaaac gcttaactcc tttctcgccg cggcggtcct gtcccttg
//
LOCUS       DATDEU010000519         5003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1123131, whole genome shotgun sequence.
ACCESSION   DATDEU010000519 DATDEU010000000
VERSION     DATDEU010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123131"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VIV63_12405"
     CDS             <1..584
                     /locus_tag="VIV63_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY6125445.1"
                     /translation="APAATQAVEAGNTLVQIVASSPDDTTNEIYATLLSVIAYAQKRI
                     DVTMAYFVPDDALETALKDAAKRGVTVRIILPSYSDFSGVFYAGRAHYDDLLESGVKL
                     YELEDAFLHSKSIVVDDVWSSIGSTNFDWRSFVHNNEISVCVIGEEFATEMTRMFAAD
                     LADSKEITLATWKKRGFRERFKELMWLPIQYWL"
     gene            complement(679..984)
                     /locus_tag="VIV63_12410"
     CDS             complement(679..984)
                     /locus_tag="VIV63_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125446.1"
                     /translation="MKPENRIERFTTHWQNTFKDEPYYSSKYSWDDFDPAYRYAYKSY
                     EENPNARFEDLETRLEAGWDIAKGKSKLAWVDAKQAIRSAWQSVERVLPGDADRDGR"
     gene            complement(1166..1714)
                     /locus_tag="VIV63_12415"
     CDS             complement(1166..1714)
                     /locus_tag="VIV63_12415"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY6125447.1"
                     /translation="MQKSKGFISAVMIVLSLAAVACASTRTTKSPGEQVDDSVTTGRV
                     KAALIADPVTKAHQIDVETFKGTVQLNGFVDTSASKERASDVAKSTKGVTAVRNNLTV
                     KTTERSGEQVVDDGAITTKVKTALAGDPRTKAHQINVETREGAVQLSGFVDSSEAKST
                     AEELARAVDNVRSVDNELDVKK"
     gene            complement(1724..1885)
                     /locus_tag="VIV63_12420"
     CDS             complement(1724..1885)
                     /locus_tag="VIV63_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY6125448.1"
                     /translation="MLHYAVIFFVIALIAAVFGFAGIAAGAAGIAKILFFVFLILAIV
                     SFIANTARR"
     gene            complement(2077..2562)
                     /locus_tag="VIV63_12425"
     CDS             complement(2077..2562)
                     /locus_tag="VIV63_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6125449.1"
                     /translation="MQDEQLRPVLDRLLATRRAKVEDTQSGARKGLRVLFTCHSGKNK
                     LLAAQLFAHELSVDLFQIDLNRIVSKYVADTEKNLDALFKAAEASDAVLLFDDTDHLF
                     GGSSSEIDESGRFPNLNVAQLRQRIESYDGVVLLGADDRKHIDPKLARQCDEVVELAD
                     D"
     gene            complement(2562..3389)
                     /locus_tag="VIV63_12430"
     CDS             complement(2562..3389)
                     /locus_tag="VIV63_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125450.1"
                     /translation="MPKPVSRVSNVRPKQAVFAASIKQFPKRLVMLTEGDSWFSYPLN
                     ANIADYIEMMSDFSLLRLEHNGDDARDILGAGSEQLKKVKYYLKNYPVDAVLLSAGGN
                     DLVAGDLAKVLNKKVAGATWQSAIKLSALTTVLDDVVAAYARLLDARDALRPKCVVVA
                     HSYCYFQPTGRKATGPFGLGKAGPWMRPVLTAKGIDPDTEGRDLARYLVDELHARLSA
                     LAATRARFQVVDMRAALPVDNVHWADEIHPSGTGFRRLAEDFWRPVLKAQFPGRGFD"
     gene            3551..>5003
                     /locus_tag="VIV63_12435"
     CDS             3551..>5003
                     /locus_tag="VIV63_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125451.1"
                     /translation="MRLFLISLLALAGAPALAAPSLTTPPTAVAGSTITLKAVGSGDP
                     REFVTVVPKGTREGGYQAYVYVTGAGDLKLAMPSAPGDYELRLCEAASPYKTMVAKPI
                     TITGASASVSAPASVAAGATVEVKWTGPNNDRDYIAIGETTPGGRLYLDYKYSRDGSP
                     LKLAAPEKPGNYEVRYILGAGDAVIARAPLVVGAVTASITGPAQVAAGAKFKVTWTGP
                     NNPRDFVTMVKTGTRERTYDRYVYTDKGATLEFTAPDEPGEYELRYATGKEYNTLART
                     PITVGAISGTVSAPAQVIAGESFKATWKGPDNPRNFITVVAKGAREGAYTSSYFYTTP
                     KNNPGNLVAPLAPGEYEIRYSTAESYLTLGRAALTVTPAKNEPGKIAVTLAAGTPSGG
                     AIEIILDASGSMLQKLGTERRIDIAKKMLTKLTSSTIPAGTPFAFRVFGREVDSCQTD
                     LDIPVGPLNPTAVGQRIAALVAKNGAKTPIGASL"
BASE COUNT          916 a         1657 c         1503 g          927 t
ORIGIN      
        1 cggcgcccgc agccacacag gcggtcgaag ccggcaatac gctcgtgcag atcgtcgcca
       61 gcagtcccga tgacaccacc aacgagatct acgccacgct gctgtcggtc atcgcgtatg
      121 cgcaaaagcg catcgacgtg accatggcct acttcgtgcc cgacgacgcg ctcgagacgg
      181 cactcaagga cgccgcgaag cgtggtgtga cggtgcgcat catcctgccg tcatacagcg
      241 atttttcggg cgtgttctac gccggccgcg cccactacga cgacctgctc gagagcggcg
      301 tcaagctcta tgaactcgag gacgcgttcc tgcactccaa gagcatcgtc gtcgacgatg
      361 tgtggtcgtc gatcggctcg accaatttcg actggcgcag cttcgtgcac aacaacgaga
      421 tcagcgtgtg cgtgatcggc gaggaattcg ccaccgagat gacgcgcatg ttcgcagccg
      481 accttgcgga ctcgaaggag atcacgctcg ccacctggaa gaagcgcggc tttcgcgagc
      541 ggttcaagga actgatgtgg ttgccgatcc agtactggtt gtgaggaatg gggacgcgcc
      601 tcatcagccg cgtcccctct tttcctcgaa gccgggaaac gggcatggcc catttcccgt
      661 gccccgctcc gccgctagct agcgaccgtc gcggtcggca tcacccggca gcacacgctc
      721 gacgctttgc caggccgagc gaatcgcctg cttcgcatcc acccacgcaa gcttcgactt
      781 gcccttggcg atgtcccagc cggcctcgag gcgcgtctcg agatcctcga agcgcgcgtt
      841 cgggttctct tcgtacgact tgtacgcata gcgatacgca ggatcgaagt catcccagga
      901 atacttcgag ctgtagtacg gctcgtcctt gaacgtgttc tgccaatgcg tggtgaatcg
      961 ttcgatgcga ttctcgggtt tcatgagtat ctcctgttgc tggaaggccg cgttatcgcg
     1021 gattcacact gtgcattcac gcgcccggca tcgaagtcgg agcgggtcgt agatacctgt
     1081 aggtcaaaca aagcggggcc ggagtgtcgc cactccggcc ccgctgtcgt cacccggatt
     1141 gccgggtctc gcttactctt cgcgcttact tcttgacgtc gagctcattg tccaccgagc
     1201 ggacgttgtc gaccgcacgg gccagttctt cagccgtcga cttcgcttcc gagctgtcga
     1261 cgaaacccga gagctgcacc gcgccttcgc gcgtttcgac gttgatctga tgcgccttgg
     1321 tgcgcggatc gccagcgagg gccgtcttca ccttggtggt gatcgcgccg tcgtcgacaa
     1381 cctgctcgcc gctgcgttca gtcgtcttca cggtgaggtt gttgcgaacc gccgtcacgc
     1441 ctttggtgct cttcgccaca tcggaagcac gttctttgct ggccgaggtg tcgacgaagc
     1501 cgttcagctg aacggtgccc ttgaacgttt cgacgtcgat ctggtgcgcc ttcgtgaccg
     1561 gatcggcaat cagcgccgct tttacgcggc ccgtggtcac cgagtcgtcc acctgctcac
     1621 caggggactt ggtcgtgcgc gtggacgcgc aagcgaccgc cgccagactc aacacgatca
     1681 tgaccgcact gataaagcct ttcgatttct gcatataaac ctcttatctc cgagccgtat
     1741 tggcaatgaa actgacgatc gcgaggatca ggaaaacgaa gaacaggatc ttcgcgattc
     1801 ccgctgcacc cgcggcaatg ccggcaaagc cgaacacggc tgcgatgagc gcgatgacaa
     1861 aaaagatgac tgcatagtga agcatcgact tctccgctgt atttgacacc gccactcggt
     1921 gccggcggtg agcatcattg ccgcaacgcg gtttcggaga tgcagggttg ctggctgatg
     1981 ccgcgtaggc gcatgaccac agggctgtaa gcccgcggga ttacaagcgt aggggcagcc
     2041 cgcttttccg attttgccag gaaaaggggg ctacccctag tcgtcggcta gttcgacgac
     2101 ctcgtcgcac tggcgcgcga gtttcgggtc gatgtgtttg cggtcgtccg cccctaacaa
     2161 cacgacgccg tcatacgact cgattctctg tcgcagctgg gcgacgttca gattcggaaa
     2221 acggcccgat tcgtcgattt ccgaactcga gccaccaaac aggtgatccg tgtcgtcgaa
     2281 cagcaatacg gcatcggatg cctcggccgc cttgaacagc gcatccagat tcttctcggt
     2341 gtcggcgacg tatttgctga cgatgcgatt gagatcgatc tggaacagat ccaccgacag
     2401 ctcgtgcgcg aacaactgcg ccgcgagcag tttgttcttg ccggaatggc aggtgaacag
     2461 cacgcgcaac cctttgcgcg cgccgctctg cgtgtcttcc accttggcgc gccgggtcgc
     2521 cagcaggcga tcgagcaccg ggcgcaattg ttcgtcttgc atcagtcgaa gccgcggccg
     2581 ggaaattgcg ccttgagcac cgggcgccag aagtcttccg ccagccggcg aaagcccgtg
     2641 ccgctgggat gtatttcgtc ggcccagtgc acattgtcca cgggcagcgc cgcacgcata
     2701 tccaccacct ggaagcgagc ccgcgtcgcg gccagcgcgc tgagccgcgc gtgaagctcg
     2761 tcgacgagat aacgcgccag gtcgcggcct tcggtgtcgg ggtcgatgcc cttggcggtc
     2821 agcaccggac gcatccacgg accggccttg ccgagcccga acgggccggt cgccttgcgc
     2881 cccgtcggct ggaaatagca gtagctgtgg gccacgacca cgcacttggg acgcagcgca
     2941 tcgcgcgcat cgagcaggcg cgcataggcc gccacgacgt cgtcgagcac cgtagttagc
     3001 gcggatagct tgatggcgct ttgccaggtc gcgcccgcga ccttcttgtt gagcaccttg
     3061 gcgagatcgc cggccaccag gtcgttgccg ccggcgctca ggagcaccgc gtccacggga
     3121 tagttcttga ggtagtactt gaccttcttg agctgctcgc tgcccgcgcc caggatgtcg
     3181 cgtgcatcgt cgccgttgtg ctcgagccgg agcagcgaga agtcgctcat catttcgatg
     3241 tagtcggcga tgttggcatt cagcggatag ctgaaccagg agtcgccttc ggtgagcatg
     3301 accagccgtt tgggaaactg cttgatgctg gcggcgaaca ccgcctgttt cggacgcacg
     3361 ttggagaccc ggctcactgg cttcggcatg tcctcaccct ccctgttctt tttcgacggc
     3421 gcactatact cccgcccctg taccggctga acggccgcgg ttgcccagtt gcagaaagcg
     3481 tgcgaaaagt cctaacaaca cggcacgggg aaactcttaa actccccggg ttgctcaacg
     3541 gagtccgacg atgcgtctct tcctgatctc cttgttagct ctggcgggcg cgcccgccct
     3601 ggcagcgccc agcctcacca ctccgcccac cgccgtcgcg gggtcgacca tcacgctaaa
     3661 agcggtcggc agcggcgacc cgcgcgagtt cgtcaccgtc gtgcccaagg gaacgcgtga
     3721 aggcggctac caggcgtatg tctatgtgac aggcgctggc gacctcaaat tggccatgcc
     3781 gtcggcgccc ggcgactacg aactgcggct gtgcgaagcc gcgagcccct acaagacgat
     3841 ggtcgcgaaa ccgatcacga tcaccggcgc cagcgccagc gtcagcgcgc ccgcctcggt
     3901 cgcggccggc gccaccgtcg aggtgaagtg gaccggcccg aacaacgatc gcgactacat
     3961 cgcgatcggt gaaaccactc ccggcggccg gctttatctc gactacaaat attcgcgcga
     4021 tggcagtccg ctgaaactcg ccgcgccgga gaagcccggc aattacgaag tgcgctacat
     4081 cctgggcgct ggcgacgcgg tcattgcccg cgcgccgctt gtggtcggcg cggtcactgc
     4141 ttcgatcacc ggccccgcgc aggtcgcggc cggcgccaaa ttcaaggtga cctggaccgg
     4201 cccgaacaat ccgcgcgact tcgtgaccat ggtcaagacg ggcacccgcg agcgcaccta
     4261 tgaccgctac gtctacaccg acaagggcgc cacgctcgaa ttcaccgcgc ccgacgaacc
     4321 cggtgagtac gagctgcgtt acgccaccgg caaggaatac aacacgctgg cccgcacgcc
     4381 cattaccgtc ggcgccatca gcggaaccgt gtccgctccc gcgcaggtga tcgccggcga
     4441 aagtttcaag gccacgtgga agggccccga caatccgcgc aatttcatca ccgtcgtcgc
     4501 caagggcgcg cgcgaaggcg cctatacgtc ttcctatttc tacacgacgc cgaagaacaa
     4561 cccgggcaac ctggtcgcgc cgctcgcccc cggcgagtac gaaattcgct actccacggc
     4621 cgagagttat ctgacgctgg gccgcgccgc gctcacggtg acgcccgcga agaacgaacc
     4681 cggcaagatc gcggtaacgc tggccgccgg caccccatcg ggcggcgcca tcgagatcat
     4741 cctcgacgcg tccggcagca tgctgcagaa gttaggcacc gaacgccgca tcgacatcgc
     4801 caagaaaatg ctgaccaagc tcacctcctc caccattcca gccggcacgc cgttcgcgtt
     4861 ccgggtattc gggcgcgagg tggattcctg ccagaccgat ctcgacatcc ctgtggggcc
     4921 tttgaatccg accgccgtgg gacagcgcat cgctgccctc gtggccaaga atggcgcgaa
     4981 gaccccgatc ggcgcctcac tcg
//
LOCUS       DATDEU010000520         6231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1123167, whole genome shotgun sequence.
ACCESSION   DATDEU010000520 DATDEU010000000
VERSION     DATDEU010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123167"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VIV63_12440"
     CDS             <1..797
                     /locus_tag="VIV63_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6125452.1"
                     /translation="LTYLGACAAALRYRVRGELALALIGGAFCIFVIGWSTLPVLIAT
                     IAGVLIAALIYICASVVIFGVAPAADIAASNAPFALAAAKMMGPIAGPLVALCGGLKA
                     RGTLAGWVLMTAQVSRAAADHGLLPQTFARTRAGDTPVAGLIIAGLLGTAIIVLTIAP
                     TLGKQFGYLAEASTLFALLTYLGACAAALRYRVRGELALALIGGAFCIFVIGWSTLPV
                     LIATIICLVLFALLYLPLSRGKYVLPDPTVRTGTQPSIRERHDDPG"
     gene            complement(794..1636)
                     /gene="fghA"
                     /locus_tag="VIV63_12445"
     CDS             complement(794..1636)
                     /gene="fghA"
                     /locus_tag="VIV63_12445"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR02821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEY6125453.1"
                     /translation="MTIETVSTHACHGGVLGYYKHRSQTNDCEMRFAVYTPPQAAAKP
                     APVLYYLAGLTCTEETFAIKAGALQHAAQHGIVLVAPDTSPRVELPGDRDSWDFGIAA
                     GFYLDATQAPWAAHYRMYSYVVDELPKIVAAHFKVDAARSGIMGHSMGGHGALTIALK
                     NPGKYKSVSAFAPIAAPIQSPWGQKAFTGYLGSDRAAWAQYDATELVKAGKKFPGAIR
                     IDQGSADKFLAEQLKPELLAAACKAAGQDLDLRMRAGYDHGYFFIQSFIAQHIAWHAH
                     RLST"
     gene            complement(1636..2211)
                     /gene="gfa"
                     /locus_tag="VIV63_12450"
     CDS             complement(1636..2211)
                     /gene="gfa"
                     /locus_tag="VIV63_12450"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HEY6125454.1"
                     /translation="MAVKLHPSIDKGLKPGAKKFKGGTLTCHCAADPVTVTIKGNVAH
                     NHACGCTKCWKPAGAKFSVVAVVSRDNLAVASNEQKLNIVDASAAIQRHACSGCGVHL
                     YGRIENTNHPFFGLDFVHVELSKEKGWAKPEFAAFVSSIIEAGVAKPREMTKIRDRLK
                     KLRLTPYDCLSPALMDAIATHVAKAKGTLKT"
     gene            complement(2239..3348)
                     /locus_tag="VIV63_12455"
     CDS             complement(2239..3348)
                     /locus_tag="VIV63_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY6125455.1"
                     /translation="MKTRAAIGWKAGQPLEVVTLDLAGPKTGEVLVEIKASGVCHTDE
                     FTRSGGDPEGLFPVVFGHEGAGIVVDVGPGVTTLKKGDHVIPLYTPECRQCKSCTSHK
                     TNLCTAIRATQGQGVMPDGTSRFSIGNEKVHHYMGCSTFSNYTVLPEIALAKIREDAP
                     FEKVCYIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVIQGARIAGANKIIGVD
                     VNPKRKAMAEKFGMTHFVNPKEVQGDLVPYLVSLTDGGADYSFECVGHVDLMRQALEC
                     THRGWGVSVIIGVAGAGQEIKTRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMDKK
                     INIDDLITHVMPIEKINDAFDLMHKGESIRSVVTF"
     gene            3446..3940
                     /locus_tag="VIV63_12460"
     CDS             3446..3940
                     /locus_tag="VIV63_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6125456.1"
                     /translation="MLIPRLRRLARPDDLVSVHRIYMHDDVVPFLGQDPMPLDGFRPV
                     FEGLLATNSFFVAVRDDEVHGFYRVNRQKGRSRHVAILETLAIAPSERGTGFAQAMIG
                     EALECLRADGVRRVELTVEADNPRALAFYQKLGFEQEGRLRAAYKRAHQAEYVDELLM
                     ARLF"
     gene            4000..4338
                     /locus_tag="VIV63_12465"
     CDS             4000..4338
                     /locus_tag="VIV63_12465"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY6125457.1"
                     /translation="MTRYLVAIHHPDGYDGSTEDEAMHRDISALNVEMVAAGVRVFVG
                     GLQPADRSKSLRSRGKGHVLVTDGPYLETKEHIGGFWVLECANLDEALAWGKKAVVAC
                     RAPVEVRPFH"
     gene            complement(4332..4670)
                     /locus_tag="VIV63_12470"
     CDS             complement(4332..4670)
                     /locus_tag="VIV63_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125458.1"
                     /translation="MKPAAATSDNWIIEHVLAVQTLISLAYFGWLIANVDLSAPLDPQ
                     RLSPLIVLVGTLCTIAAVVFWVRMFRDFFRRRPERHAAAWGWFLLLAGHLAALFYFLW
                     IWRPRNRPGQ"
     gene            complement(4667..>6231)
                     /gene="edd"
                     /locus_tag="VIV63_12475"
     CDS             complement(4667..>6231)
                     /gene="edd"
                     /locus_tag="VIV63_12475"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEY6125459.1"
                     /translation="HRPFEPYPEVIRAAARVAGATAQVAGGVPAMCDGVTQGRAGMEL
                     SLFSRDVIALATALALSHEAFDGSLLLGICDKIVPGLFIGAASFGQMPIIFVPGGPMP
                     SGMANKAKAEVRQRYAEGLATRDELLAAEMGSYHDAGTCTFYGTANSNQMLMEMMGLH
                     VPGAAFVAPNTPLRHALTVAATQRVAQTTALGANYLPLVEVIDERSFVNAIVGLMATG
                     GSTNHVIHLVAMARAVGLSVTWDDMADISAITPLIARVYPNGEADVNQFHAAGGMAFV
                     VRELINAGLAHESAMTVWGPGLSAYAKDPFLVDNKLVWRDAADKSHDEKILRSASNPF
                     SADGGLKLLRGNLGRSVIKTSAVDPKYWDITAPARVFESQEAMAAALKAGIDGDFIAV
                     VRGQGPRANGMPELHKLIPACSALLAKGRRVAIVTDGRMSGASGKVPAAIHLTPEAAA
                     DGPISRLRDGDIIRLDARAGVLEVQVEAATLQAREAIKPELFDTTFGVGRELFTRLRE
                     GASAADEGASIL"
BASE COUNT         1049 a         1956 c         2074 g         1152 t
ORIGIN      
        1 tgctcaccta tctcggcgcc tgcgcggcgg cactgcgtta tcgcgtacgc ggagaattgg
       61 cgctggcgct catcggcggc gcgttctgca tattcgtgat cggctggtcg acgctgccgg
      121 tgctcatcgc gacgatcgcc ggcgtgctca ttgccgcact catatatata tgtgcgagcg
      181 tcgtgatctt cggtgttgcg ccagccgccg acatcgcggc gtccaacgca ccgttcgcgc
      241 tcgcggccgc aaagatgatg gggcccatcg ccggtcccct ggtggcgttg tgtggtggcc
      301 tcaaggcgcg gggcacgctg gcgggttggg tgctcatgac agcgcaggtg agccgcgcgg
      361 ccgccgatca cggcctgctg ccgcaaacgt tcgcgcgcac gcgcgcgggc gataccccgg
      421 tggcgggcct catcatcgcc ggcttgttag gcacggcgat catcgtgctg accatcgccc
      481 ccacgctcgg caaacagttc ggctacctgg ccgaagcgtc gacgctgttc gcgctgctca
      541 cctatctcgg cgcctgcgcg gcggcactgc gttatcgcgt acgcggagaa ttggcgctgg
      601 cgctcatcgg cggcgcgttc tgcatattcg tgatcggctg gtcgacgctg ccggtgctca
      661 tcgcgacgat catctgcctg gtgttgttcg ccttgctcta cctgccgctg tctcgcggca
      721 agtacgtctt acccgatccc acggtgcgca ccgggacgca accgtcgatt cgcgaacgtc
      781 acgacgatcc gggctaggtg ctcaggcgat gcgcgtgcca ggcgatgtgc tgcgcgatga
      841 aactctggat gaagaaatag ccgtggtcgt aacccgcgcg catgcgcagg tcgagatcct
      901 gacccgcggc cttgcaggcg gcggctaaca actcgggctt cagctgctcg gcgaggaatt
      961 tgtccgccga cccctgatcg atgcggatgg caccggggaa tttcttgccg gccttcacga
     1021 gttccgtggc gtcgtactgc gcccaggcgg cgcgatcgct gcccaggtag ccggtgaacg
     1081 ccttctggcc ccacgggctc tggataggcg ccgcgatggg tgcgaacgcc gacacggatt
     1141 tgtacttgcc gggattcttg agcgcgatcg tcagcgcgcc atgtccgccc atggagtgac
     1201 ccatgatgcc gctgcgcgcc gcgtcgacct tgaaatgggc cgcgacgatc ttcggtaact
     1261 cgtccaccac gtagctgtac atgcggtagt gcgcggccca gggcgcctgc gtggcgtcga
     1321 gatagaaccc ggcggcaatg ccgaagtccc acgaatcgcg atcgccgggt agttcgacgc
     1381 gcggactggt gtccggcgcc accagcacga tcccgtgctg ggccgcgtgc tgaagcgcgc
     1441 cggccttgat ggcgaaagtc tcttcggtgc aggtgagacc cgcgaggtaa tacaacaccg
     1501 gcgcaggctt ggcagcggcc tgcggcggtg tgtagacggc gaagcgcatc tcgcagtcgt
     1561 tggtctgcga gcgatgcttg tagtagccca gcacgccgcc gtggcaggcg tgcgtagaaa
     1621 cggtttcgat ggtcatcaag tcttcaacgt tcccttcgcc ttggcgacgt gtgtggcaat
     1681 ggcgtccatg agcgccggcg acaggcagtc gtaaggcgtg aggcgcagct tcttcaggcg
     1741 gtcgcgaatc ttggtcattt ccctgggttt ggcgacaccc gcttcgatga tggaggacac
     1801 gaacgccgcg aactccggct tcgcccagcc cttctccttc gacagctcca cgtgcacgaa
     1861 gtcgagaccg aagaacggat ggttcgtgtt ctcgattcgt ccgtacaggt gcacgccgca
     1921 gccgctgcac gcgtgccgct ggatggcggc gctggcatcg acgatgttca gtttctgttc
     1981 gttggaggcg accgcgaggt tgtcgcggct cacgacggca accaccgaga acttcgcacc
     2041 cgcaggcttc cagcacttgg tgcagccgca ggcgtggttg tgcgccacgt tgcccttgat
     2101 ggtgacggtc accgggtcgg cggcgcagtg gcaggtgagc gtgccgccct tgaacttctt
     2161 ggcgccgggt ttgagacctt tgtcgatcga tggatgaagc ttgacggcca tgaccattcc
     2221 ctcgtcgttg ttagttgttc agaaggtaac tacggagcga atcgactcgc ccttgtgcat
     2281 gagatcgaaa gcgtcgttga tcttctcgat tggcatgaca tgcgtgatga ggtcgtcgat
     2341 gttgatcttc ttgtccatgt accagtcgac gatcttcggc acgtcggtgc gcccgcgcgc
     2401 accgccgaac gcggttcctt tccagacacg gcccgtgacc agctgaaacg gccgcgtctt
     2461 gatttcctga ccggcgccgg cgacaccgat gatcaccgac acgccccacc cgcgatgcgt
     2521 gcactcgagc gcctggcgca tgagatcgac atggcccacg cactcgaagg agtagtccgc
     2581 acctccgtcg gtgagcgaga cgaggtacgg cacgaggtcg ccctgcacct ctttcgggtt
     2641 cacgaagtgc gtcatgccga atttttcggc catggccttt cgcttcgggt tcacgtcgac
     2701 gccgatgatc ttgttggcgc ccgcgatgcg cgcgccctgg atgacattga ggccgatgcc
     2761 accgagaccg aacacgacga cattcgcgcc cggctccacc ttcgcggtgt tgatgacggc
     2821 gccgataccg gtggtgacac cgcagccgat gtagcagacc ttctcgaacg gcgcgtcctc
     2881 gcggatcttc gccagcgcga tctccggcaa cactgtgtag ttagagaacg tgctgcaacc
     2941 catgtagtga tgcaccttct cgttgccgat ggagaagcgg ctggtgccat cgggcattac
     3001 gccctggccc tgcgtggccc ggatcgcggt gcacaggttc gtcttgtgag aggtgcagga
     3061 tttgcactgg cggcattccg gcgtgtacag cgggatgacg tgatcgccct tcttcagcgt
     3121 ggtgacgccg gggccgacgt caacaacaat acccgcgccc tcgtgaccga acaccaccgg
     3181 gaacaaccct tccggatcgc cgcccgagcg cgtgaattca tcggtgtggc agacgccgga
     3241 cgccttgatt tccaccagca cttcgccggt cttgggaccc gcgagatcca gcgtaacgac
     3301 ttcgagtggc tggccggctt tccagccgat ggcggcgcgc gtcttcatcg ttctttgccc
     3361 cttccccgtg aggcggcgat ggtaccgcga tgggacgggc atcggccatt gcggcgcgaa
     3421 aggccgcctc atacagttgc gcaacatgct gattccgcgc cttcgccgac tcgcccgtcc
     3481 ggatgacctc gtgtcggtcc atcgcatcta catgcacgac gatgtcgtgc cgttcctggg
     3541 gcaggacccg atgccactcg acggcttccg tccggtgttc gagggattgc tggcaacgaa
     3601 ctcgtttttc gtcgcggtgc gtgatgatga agtgcacggg ttctatcgcg tgaaccggca
     3661 gaagggccgg tcgcggcacg tcgcgatcct ggagacgctg gcgatagccc cgtccgaaag
     3721 aggcaccggc ttcgcgcagg cgatgatcgg cgaagcgctg gagtgcctgc gtgcggatgg
     3781 cgtccggcgc gtggagctca cggtcgaggc cgataacccg cgcgcgttag cgttctatca
     3841 aaagctgggg ttcgagcagg aaggacggct gcgcgcggcg tacaagcgtg cgcaccaggc
     3901 ggagtatgtc gatgagctgc tgatggcgcg tctgttctga tttgtcgaat ccggcgccgg
     3961 ccgttcgtct agaaagcgaa ggccaataca ggaggaatca tgacgcggta tctggttgcc
     4021 attcaccacc ccgacggcta cgacggctcg acggaagacg aagcgatgca tcgggacatc
     4081 agtgcgctga acgtcgagat ggtcgcggcg ggagtccgcg tcttcgtggg cggtcttcag
     4141 ccggctgatc gctcgaagtc actgcggtcg cggggcaagg gccacgtgct cgtcaccgac
     4201 gggccgtacc tggaaaccaa ggaacacatc ggtggtttct gggtgctcga atgcgcgaac
     4261 ctggacgaag cgctggcgtg gggcaaaaaa gccgtcgtcg catgccgggc accggtcgaa
     4321 gtgcgtccgt ttcactgacc cggacgattg cggggccgcc agatccagag aaagtagaac
     4381 agcgccgcga gatgtccggc cagcaacagg aaccagcccc aggcggccgc atgccgctcc
     4441 ggtcgccggc gaaagaaatc cctgaacatg cggacccaga acaccacggc ggcgatggtg
     4501 cacagcgtgc cgaccagcac gatgagcggc gagaggcgtt gcggatcgag cggtgcgctc
     4561 aggtcgacgt tggcgatgag ccacccgaag tacgccagcg agatcagcgt ctgaaccgcg
     4621 agcacatgct cgatgatcca gttgtcgctg gtcgcggcag cgggcttcac aggatggacg
     4681 cgccctcgtc ggcggcgctg gcgccttcac gcagacgcgt gaacagctcg cgcccgacgc
     4741 cgaaggtggt gtcgaacaac tcgggcttga tggcttcgcg cgcctgcaat gtggcggcct
     4801 cgacctggac ctcgagcact cccgcgcgcg catcgaggcg gatgatgtcg ccatcgcgca
     4861 aacgcgagat cggaccgtcc gccgccgcct cgggcgtgag atggatggcc gcgggcacct
     4921 tgcccgaagc gccggacatc cgtccgtcgg tgacgatggc cacgcgccgc cccttggcta
     4981 acaaggcgga gcacgcggga atcagtttgt gcagctcggg cattccattg gcgcgcgggc
     5041 cctgcccacg gaccacggca atgaagtcgc catcgatgcc ggctttgagt gcggcggcca
     5101 tggcttcctg gctttcgaac acgcgtgcgg gtgcggtgat gtcccagtat ttcggatcga
     5161 cggccgaagt cttgatgacc gaacggccga gattgccgcg cagcagcttg aggccgccgt
     5221 ccgcggaaaa cgggttcgac gcggagcgca ggattttttc gtcgtgcgat ttgtcggcgg
     5281 catcgcgcca cacgagtttg ttgtcgacga ggaatgggtc cttggcgtaa gccgaaagcc
     5341 ccggacccca gaccgtcatg gctgattcgt gcgcgaggcc tgcattgatg agttcgcgca
     5401 cgacgaaagc catgccgccc gcggcgtgaa actggttcac gtccgcttca ccgttcgggt
     5461 agacgcgcgc gatcaacggc gtgatggcgg agatgtccgc catgtcgtcc caggtgacac
     5521 tgagcccgac ggcacgcgcc atggcgacga ggtggatcac gtgattggtc gaaccgcccg
     5581 tggccatgag tccgacgatg gcgttgacga acgaacgctc gtcgatgact tcgacgagcg
     5641 gcaggtagtt ggcgcccagc gcggtggtct gcgcgacccg ctgagtggct gcgaccgtga
     5701 gcgcatggcg cagcggcgtg ttcggcgcga cgaacgcagc gccgggcaca tgcagtccca
     5761 tcatctccat gagcatctgg ttggaattgg cggtgccgta gaacgtgcag gtgccggcgt
     5821 cgtggtacga acccatttcg gcggccagca attcatcgcg cgtggcgagg ccttctgcat
     5881 agcgctgacg cacttcggcc ttcgccttgt tggccatgcc gctcggcatc gggccgccag
     5941 gcacgaagat gatgggcatc tggccgaacg atgccgcgcc gatgaacagg cccggcacga
     6001 tcttgtcgca gatgcctaac aacagggaac catcgaaagc ttcgtgactc agagccaacg
     6061 ccgtcgccaa tgcgatgacg tcgcgactga acagcgagag ctccatgccg gcgcgaccct
     6121 gcgtcacgcc atcgcacatg gcgggcacgc cacccgcgac ctgggccgtg gcgccggcga
     6181 ctcgcgccgc ggcacggatc acctcgggat agggctcgaa cggccgatgt g
//
LOCUS       DATDEU010000521         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1123670, whole genome shotgun sequence.
ACCESSION   DATDEU010000521 DATDEU010000000
VERSION     DATDEU010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123670"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="VIV63_12480"
     CDS             complement(<1..2020)
                     /locus_tag="VIV63_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6125460.1"
                     /translation="MAETITVTGQSPLVETSSNTLSKTITRTELDTLPLSGRNFSNLA
                     AMSPGVAMVGNSNNNVLAAGQTNRSNSFLIDGTSNDDTTVATARGGFSLEAVREFAVM
                     ANQFSAEYGMASGAIVSVVTRSGTNDMQGRLFLFHRDDSFDAQDPFSKAQGSGKSPFS
                     QQRYGGFLGGPLVRDRFHYFGSFEGLRERSTNVITSSLVPADQREEPATSDGDQYFFK
                     TDLRFTGNHAMTARYRADDRISTGNGIGGLNTRERGSNTAGLDQDVVTSFTSVLTNRA
                     LNEVRVQYAMRESFTDVNGYSADGMPQINRPSGNFGKNQANPQGRNENRLQIVENFSY
                     SLNTHNFKLGADVSIIRADSFFPRNRDGNFTFTTDAPFDPNNLATYPTQYVVATVDPT
                     VDLPNDLYSFFVQDQWQLQTNLTLNLGLRYDRETGFSKITDVPDDSNNFQPRLGVVWD
                     PFKDGKTAVRGGYGHYVDQSFLNIQLNVASAKGAVETVIANPGYPDPYSRGTTAATPP
                     SITTLTPTPDTPETRTASLGVKREIVPGFAVSVDGVYARGYNQYAWLDLNYPDPVTGL
                     RPDTTRGRITEYADYGNSWYSALLVGVEGRQSAKLNWNGSYTWSKNERDVEGFQFTAQ
                     DMNNLGADRGLASNHRAHQFIGNVTYQMPWGFQIGAIAQARSGTPWNVT"
BASE COUNT          310 a          603 c          708 g          411 t
ORIGIN      
        1 tcgtgacgtt ccagggagtt ccggagcgcg cttgcgcgat cgcaccaatc tggaatcccc
       61 atggcatttg ataggtcacg ttgccgatga actggtgggc gcggtgatta ctggccagac
      121 ccctgtccgc gccgagattg ttcatgtcct gggcggtgaa ctggaagccc tcgacgtcgc
      181 gttcgttctt ggaccaggta tacgacccgt tccagttcag cttggcgctc tgccggcctt
      241 cgacgccgac cagcagcgcc gagtaccacg agttgccgta atcggcatat tcggtgatgc
      301 ggcccctcgt cgtgtcggga cgcaggcccg tcaccggatc cggataattc agatccagcc
      361 aggcgtactg gttgtagccc ctcgcataca cgccgtcgac ggacaccgcg aagcctggca
      421 cgatctcgcg cttcacgccg aggctggcgg tgcgggtttc cggcgtgtca ggggtcggcg
      481 tgagggtcgt gatgctcggg ggcgtcgccg cggtggtgcc gcgcgagtat ggatccgggt
      541 atcccgggtt ggcgatcacc gtttcgaccg cgcccttcgc cgaggcgacg ttgagttgga
      601 tattgaggaa gctctggtcg acgtagtggc cgtagccgcc ccgcaccgcg gtcttgccgt
      661 ccttgaaggg atcccacacc acgccgagcc gcggctggaa gttgttgctg tcgtcgggca
      721 cgtcggtgat cttgctgaag ccggtctcac gatcgtagcg gaggccgagg ttcagcgtca
      781 ggttcgtctg cagctgccac tgatcctgca cgaagaacga gtagaggtcg ttggggagat
      841 cgacggtcgg atccaccgtc gccaccacgt actgcgtcgg atacgtggcg agattgttcg
      901 gatcgaacgg cgcgtccgtg gtgaacgtga aattgccgtc gcggttacgc gggaagaagc
      961 tgtcggcgcg gatgatgctg acgtcggcgc cgagcttgaa gttgtgcgtg ttgagcgagt
     1021 agctgaagtt ctcgacgatc tgcaggcggt tctcgttgcg gccctgcggg ttcgcctggt
     1081 tcttgccgaa gttgccagag ggccggttga tctgcggcat gccgtccgcg gaatagccgt
     1141 tgacgtcggt gaacgactcg cgcatcgcgt actgcacgcg cacttcgttg agggcgcggt
     1201 tggtcagcac cgacgtgaag ctcgtgacca cgtcctggtc gaggccagcg gtgttggagc
     1261 cgcgctcacg ggtgttgagc ccgccgatgc cgttgccggt cgagatgcga tcgtcggcgc
     1321 ggtagcgcgc cgtcatcgcg tggttgccgg tgaagcgcag gtcggtcttg aagaaatact
     1381 gatcgccgtc gctcgtggcc ggttcttcgc gctggtcagc cggcaccagc gacgaggtga
     1441 tgacgttggt gctgcgctcg cgcaggcctt cgaacgaccc gaagtagtga aagcgatcgc
     1501 gcaccagcgg accgccaagg aagccgccgt agcgctgctg gctgaatggc gacttgcccg
     1561 agccctgcgc cttcgagaac ggatcctgcg cgtcgaagct gtcgtcgcga tggaacagga
     1621 acagccggcc ctgcatgtcg ttggtgcccg accgcgtgac cacgctgacg atggcgccgg
     1681 aggccatgcc gtattcggca ctgaactggt tcgccatcac ggcgaattcg cgcaccgctt
     1741 cgagcgagaa gccgccgcgc gcagtcgcca cggtggtgtc atcgttgctc gtgccgtcga
     1801 tcaggaagct gttgctgcgg ttggtctggc cggccgcgag cacgttgttg ttgctgttgc
     1861 cgaccatcgc cacgccgggc gacatcgccg caaggttgga gaagttgcgg ccgctcagcg
     1921 gcagcgtgtc gagctcggtg cgcgtgatgg tcttcgacag cgtgttgctg ctggtctcaa
     1981 ccagcggtga ctgcccggtg acggtgatcg tctctgccac ggtggccagt tt
//
LOCUS       DATDEU010000522         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_684674, whole genome shotgun sequence.
ACCESSION   DATDEU010000522 DATDEU010000000
VERSION     DATDEU010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684674"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..>2288
                     /gene="metH"
                     /locus_tag="VIV63_12485"
     CDS             63..>2288
                     /gene="metH"
                     /locus_tag="VIV63_12485"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY6125461.1"
                     /translation="MNSRGDDLRKLFAERIVILDGAMGTMIQQHKLDEAAFRGERFKD
                     WPHDLKGCNDLLVLTKPELIEDIHGQFLEAGADLIATNTFNATSLSLADYHLEELVYE
                     LNREAVKLARRVADTQSKKLGRTCYVAGGLGPTSKTASISPDVNDPGFRAVTFDQLVA
                     TYSDATRGLIDGGADAILIETIFDTLNAKAAIYAVRDTLDKAGLDLPIIISGTITDAS
                     GRTLSGQTVEAFWASVRHARPITISLNCALGVKQLRPYVEELARLADTNVGVYPNAGL
                     PNAFGEYDEAPEDTAKVLGELAHAGHLNFVGGCCGTTPAHIKAIADRVRCEPVRKKKE
                     HVPRLELSGLEAFNLTQDIRFVNVGERTNVTGSAKVRKLIEANDYPAALSIARQQVQD
                     GAQIIDVNMDEGMLDSEAAMVRFLNLIAAEPDIARVPVMIDSSKWTVIESGLKCIQGK
                     GVVNSISLKEGEEAFLVHARKVMAYGAAVVVMAFDEKGQADTLTRKVEICERSYKLLV
                     GIGFPPEDIIFDPNIFAIATGIDEHNNYGVDFIEATREIRKRCPHAHISGGVSNVSFS
                     FRGNEPVRQAMHSVFLYHAIQAGMDFGIVNAGQLAVYDDIPKDLRERVEDVILNRRAD
                     ASERLLEIAVNYKGGGAQERKEDLAWRQLPVRERLSHALVSGNDQYILEDTEEARLAA
                     EHPLHVIEGALMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLSPFIEALADGVQ
                     KT"
BASE COUNT          460 a          744 c          710 g          374 t
ORIGIN      
        1 ccacgcgctc ggcgtgcgac cgtcgtccgc aagggatggg gcatcgtcac gcaaggcggc
       61 caatgaatag tcgcggcgac gatctgcgaa agctgttcgc ggagcgcatc gtcatcctcg
      121 acggcgccat gggcaccatg atccagcagc acaagctgga tgaggccgca ttccggggcg
      181 agcgcttcaa ggactggccg cacgacctca agggttgcaa cgacctgctg gtgctcacga
      241 agcccgagct catcgaagat attcacgggc agttcctcga agccggcgcg gacctcatcg
      301 cgacgaacac cttcaatgcg acctcgctct cgctggccga ctatcacctc gaagaactcg
      361 tgtacgagct caaccgcgaa gcggtgaaac tcgcgcgccg cgtcgccgat acgcagtcga
      421 agaagttggg ccgcacctgc tacgtggccg gtggcctggg cccgaccagc aaaaccgcct
      481 ccatttctcc cgacgtcaac gacccgggct ttcgcgcggt cacgttcgac cagctggtgg
      541 caacctacag cgacgccacg cgcggcctca tcgacggcgg cgcggacgcc atcctcatcg
      601 aaaccatctt cgacacgctg aacgccaagg cggctatcta cgcggtgcgg gatacgctgg
      661 acaaggcggg tctggacctg ccgatcatca tctccggcac gatcaccgac gcttcgggac
      721 gcacgctctc gggccagacg gtcgaggcgt tctgggcttc ggtgcgccat gcgcgcccga
      781 tcaccatcag tctcaactgc gcgctcggcg tcaaacagct tcggccgtat gtcgaagagc
      841 tggcgcgact cgcggacacc aacgtcggtg tctatcccaa cgccggtctg cccaacgcgt
      901 ttggcgaata cgacgaagcg cccgaagaca ccgccaaggt gctcggcgaa ctcgcgcatg
      961 ccggccacct gaatttcgtc ggcggctgct gcggcacgac cccggcgcac atcaaggcaa
     1021 tcgccgatcg cgtgcgctgc gagccggtgc gcaagaagaa agaacacgtg ccgcgtctcg
     1081 agctctccgg actcgaagcc ttcaacctca cgcaggacat ccgtttcgtg aacgtgggcg
     1141 aacgcaccaa cgtcacgggc tcggccaagg tccgcaagct catcgaggcc aacgactatc
     1201 cggccgcgtt gtcgatcgcg cgtcagcaag tgcaggacgg cgcgcagatc atcgacgtga
     1261 acatggacga gggcatgctc gattcggaag cggccatggt gcgcttcctg aatctcatcg
     1321 ccgccgagcc cgacatcgcg cgcgtgccgg tcatgatcga ctcttcgaag tggacggtca
     1381 tcgaatccgg cctgaagtgc atccagggca agggcgtggt gaattccatc tcgctcaagg
     1441 aaggcgaaga agcgttcctc gtgcatgcgc gcaaagtcat ggcgtacggc gcggccgtgg
     1501 tcgtcatggc cttcgacgag aagggccagg ccgatacgct cacccgcaag gtcgagatct
     1561 gcgagcgcag ctacaagttg ctcgtgggca tcggcttccc gccggaagac atcatcttcg
     1621 atccgaacat cttcgcgatc gccacgggca tcgacgaaca caacaactac ggcgtggact
     1681 tcatcgaagc cacgcgcgag atccgcaagc gttgcccgca tgcgcacatt tccggcggcg
     1741 tgagcaacgt atcgttctcg ttccgcggca acgagccggt gcgccaggcc atgcattcgg
     1801 tgttcctata ccacgcgatt caggcgggca tggacttcgg catcgtcaac gccggccagc
     1861 tcgcggtgta cgacgacatc ccgaaagatc ttcgcgagcg cgtcgaggat gtaatcctca
     1921 atcgacgggc cgatgcgtcg gagcgtctgc tcgagatcgc cgtgaactac aagggcggcg
     1981 gcgcgcagga gcgcaaggaa gatctggcgt ggcggcaatt gccggtgcgc gaacgcctga
     2041 gccacgcgct cgtgtcgggt aacgatcagt acatcctcga agacaccgag gaagcacgcc
     2101 tcgcggcgga acatccgctg cacgtcatcg aaggcgcgct catggacggc atgaacgtgg
     2161 tcggcgacct gtttggttcg ggcaagatgt tcctgccgca ggtagtcaaa tccgcccgtg
     2221 taatgaaaaa ggccgtggcg catctcagtc cgttcatcga agcgctggcc gacggcgtgc
     2281 agaagacc
//
LOCUS       DATDEU010000523         5158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2142963, whole genome shotgun sequence.
ACCESSION   DATDEU010000523 DATDEU010000000
VERSION     DATDEU010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142963"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VIV63_12490"
     CDS             complement(<1..303)
                     /locus_tag="VIV63_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125462.1"
                     /translation="MRRIHGYRWPAALICLVFLAPAFAAKSTLPKGTKLPKDVKVPND
                     TPVAIYLPANDLGTRFYVSSIGAWAYPGKSLDDARRELGGQLFPQMRSVALAESGEF"
     gene            377..1897
                     /gene="ilvA"
                     /locus_tag="VIV63_12495"
     CDS             377..1897
                     /gene="ilvA"
                     /locus_tag="VIV63_12495"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY6125463.1"
                     /translation="MPDTYIERILRARVYDVATETPLESAPRLSRRIGNTVLLKREDM
                     QPVFSFKLRGAYNKISLLSDSVAKRGVICASAGNHAQGVALAAKRRGIPAVIVMPQTT
                     PNIKVSAVRDLGGEVVLHGDDYDSAYEHAQSIVRERNLAFVHPFDDQDVIAGQGTIAV
                     EVLRQSRGEVDAIFVPVGGGGLIAGIAVYAKYFNPKIRIIGVEPVDAAAMHDSLRAKK
                     RVVLDRVGVFADGVAVKKVGEESFRLCREHVDEIVLVDTDEICAGMQDIFEDTRSIVE
                     PSGALAVAGIKKYCALNNWQDKRVVAINSGANMNFDQLRYVAERSDVGQSREALLAVE
                     IPEQPGSFLKFLEILGQRAVTEFNYRHGAKERAQIFVGFSLARGKEERDEVIMKLRAA
                     GYGVTDMTDNEIAKLHVRYMVGGHVNSGVSSERLYRFEFPERKGALLKFLQSVGTRWN
                     ITLFHYRNHGSDYGRVLAGIDVPSSDTAEFLDHLNVLHYSYSDETNNPAYRIFLGA"
     gene            2042..2272
                     /locus_tag="VIV63_12500"
     CDS             2042..2272
                     /locus_tag="VIV63_12500"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEY6125464.1"
                     /translation="MAKRIAIGIVGGSVLVVGVCMIVLPGPAFVVIPVGLGILGLEFA
                     WARIWLKKAKAKAEEVARSFKSNRNGDPPAAP"
     gene            complement(2273..4081)
                     /locus_tag="VIV63_12505"
     CDS             complement(2273..4081)
                     /locus_tag="VIV63_12505"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY6125465.1"
                     /translation="MKTVSGTVVAGIAAILLCACAPAPPRADPPPPPPPPPPTADQQF
                     EALAQRYLKEGPEQSPVGATALGDHRFDDRLDDVSVAGWQAREAFAEAYLAALGPIDV
                     TRLSRGNQVDAALLRNDLQYDQWKYQTLQDFRWNPLIYTRIAGDALYNLLAREFAPAN
                     ERMKNLGKRLDEMPRFLAQVREVLEPARVPKVHAETAAKQNAGLISMLDGQIAEEIAR
                     LPADEQTSLRASVAKARTALSQHQIWLDKRLTPEAKGDFRLGAAFYDQKLAYALFSPL
                     SREEIRERAQSELAATRTAMYEIAATVLKGKRGSPSTPEKPSASQQQRAIKAALELAY
                     KERPDRDAVLDTARATLVDATQFVRDKNIVTVPDEPLEIIVMPEFQQGVALAYCDSPG
                     PLESAQKTFYAVSPIPAKWTRAQTDSFLREYNTRSIHNLTIHEAMPGHYLQLAHSNKY
                     PSTLRKLLASGPFIEGWAVYTERVMVEQGYMNGDPLMKLIQLKWYLRTIVNALLDQAV
                     HVDGISREAAMKLMTESGFQEEREAAGKWTRAQLTSAQLPVYFVGAQEHIALRAEAEQ
                     RLGGGFAMREYHDKVLSYGSPPVRFARALMFDLPVE"
     gene            4109..>5158
                     /locus_tag="VIV63_12510"
     CDS             4109..>5158
                     /locus_tag="VIV63_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6125466.1"
                     /translation="MVNGARRYQLKCATPNTRVLHPTTLISHSRSPGRQALSHPDFAR
                     YAYGRFAATLAWQMIDVVLGYQVWELTRKVEYLGLIGLAQFLPFVILLLPGGQVADRF
                     DRRLIISLAYGLELIAAVALLWFTLFSRHEVILLFFIAALLGVGRSFWAPAGQAMVPN
                     LVPKELLSGAISMNTLMFTIATISGPAIAGLTLILGIDWSYGIAVALLIVAIFMMLGV
                     KPVLAKSVSEWRWGDVLGGFVFVWRKKPVLGAISLDLFAVLFGGVVALLPAYADKILH
                     VGPVGLGIMRAAPGVGAAIVAFWLGMRPIQRHAGTWMFGGVALFGVCMITVGLSTSIW
                     LTIVALIIAGAGDMVS"
BASE COUNT          852 a         1636 c         1667 g         1003 t
ORIGIN      
        1 gaactccccg gactcggcga gtgccacgga ccgcatctgc ggaaaaagct gcccgccgag
       61 ctcgcgccgc gcatcgtcca gcgactttcc ggggtaggcc caggcgccga tgctgctgac
      121 gtagaaccgg gtgcccaggt cgttggccgg tagatagatg gcgacgggcg tgtcgttcgg
      181 caccttgacg tctttcggca gcttcgtgcc cttgggcaag gtgctcttcg cggcgaacgc
      241 cggcgccagg aacaccaggc atatcagggc ggccggccac cggtatccgt gaattcttcg
      301 catgtttttg ctcatctccc tgcgcggcga ttctagtcta ctgtgccgcg gcgggcgcgc
      361 cggttaagct aggtgcatgc ctgataccta catcgaacgc atccttcgtg cccgcgtcta
      421 cgacgtcgcc acggaaacac cgctggaatc cgcgccgcgc ctctcgcgcc gcatcggcaa
      481 tacggtgctc ctgaagcgcg aagacatgca gccggtgttc tcgttcaagc tgcgtggcgc
      541 ctacaacaag atctcgctcc tgtccgattc ggtggcgaag cgcggggtca tctgcgcgtc
      601 ggcgggcaac cacgcccagg gtgtggcgct ggcggcgaag cgccgcggca tacccgcggt
      661 catcgtgatg ccccagacca cgcctaacat caaggtcagc gcggtgcgcg acctcggcgg
      721 cgaagtggtc ctgcatggcg acgactacga cagcgcctac gagcacgcgc agtcgatcgt
      781 gcgcgagcgc aacctcgctt tcgtgcatcc gttcgacgac caggacgtga tcgcggggca
      841 gggcaccatc gcggtggaag tgctccgcca gtcgcgcggc gaggtggatg ccatcttcgt
      901 acccgtgggc ggaggcggac tcatcgccgg catcgcggtt tacgcaaagt acttcaaccc
      961 gaagatccgc atcatcggcg tcgagccggt cgatgccgcg gccatgcacg attcactgcg
     1021 cgccaagaaa cgcgtcgtgc tcgaccgcgt gggtgtgttc gccgacggcg tcgcggtcaa
     1081 gaaggtaggc gaggaatcct tccggttgtg ccgtgagcac gtcgatgaaa tcgtgctcgt
     1141 cgacaccgac gaaatctgcg ccggcatgca ggacatcttc gaggacacgc gctccatcgt
     1201 cgaaccgtcg ggagcgctgg ccgtcgcggg catcaagaag tactgcgcgc tcaacaactg
     1261 gcaggacaag cgcgtcgtcg ccatcaacag cggcgccaac atgaacttcg accagctgcg
     1321 ctacgtcgcc gaacgctccg acgtggggca gtcgcgcgag gcgctgctcg ccgtggagat
     1381 tcccgagcag ccgggcagct tcctcaagtt cctggaaatc ctgggtcagc gtgcggtcac
     1441 cgaattcaac tatcggcatg gcgcaaaaga gcgcgcgcag atcttcgtgg gtttttcgct
     1501 ggctcgaggc aaggaagagc gcgacgaagt catcatgaag ctgcgtgccg ccggctacgg
     1561 cgtcaccgac atgaccgaca acgagatcgc gaagctgcac gtgcgttaca tggtgggtgg
     1621 tcacgtcaac agcggcgtgt cgagcgaacg gctctatcgc ttcgaatttc cggagcgcaa
     1681 aggcgcgctg ctgaagttcc tgcagtcggt gggaacgcgc tggaacataa cgctgttcca
     1741 ctatcgcaac catggttctg actacgggcg tgtactggcc ggaatcgatg tgccgtcgtc
     1801 cgacacggcg gaattcctgg atcacctcaa cgtgctgcac tattcctaca gcgatgagac
     1861 taacaatccg gcttaccgga tttttctcgg cgcctgagcg cccgatttgc tcatgaggaa
     1921 atagtcaggt cccgggggaa cacgggccgg taacaccggt ctgtgtatga tgcctcccgc
     1981 cgccgtcatc aggtctctat tggtgttcga cgaaaagcac cctctttacg ttccctatcg
     2041 catggccaaa cgtattgcca tcggcatcgt cggcggttcg gtgctcgtcg tcggcgtatg
     2101 catgatcgtg ctgcccgggc ccgcgttcgt ggtcatcccg gtgggtctcg gcatcctcgg
     2161 cctcgaattc gcgtgggcac gcatctggct caagaaagcc aaggccaaag ccgaagaagt
     2221 ggcgcgcagc ttcaagagca acaggaacgg cgacccgccg gccgctccct aactactcca
     2281 ccggcaggtc gaacatcagc gcccgcgcga agcgcaccgg cggcgatcca tacgacagca
     2341 ccttgtcgtg gtactcacgc atcgcaaagc ccccgcccag ccgctgttcg gcctcggcgc
     2401 gtaatgcgat gtgttcctgg gcgccgacga agtagaccgg tagttgggcc gaggtgagct
     2461 gcgcgcgcgt ccacttgccc gcggcttcgc gctcctcctg gaatcccgac tcggtcatga
     2521 gcttcatcgc cgcttcgcgc gagatcccgt cgacgtgtac cgcctggtcg agcagcgcgt
     2581 tgacgatggt gcgcaggtac cacttgagct ggatgagttt catgagcgga tcgccgttca
     2641 tgtaaccctg ttcgaccatc acgcgctcgg tgtaaaccgc ccagccctcg atgaacggac
     2701 cggacgccag cagcttgcgc aatgtcgagg gatacttgtt ggagtgcgcg agctgtagat
     2761 agtggcccgg catcgcttcg tgaatggtca ggttgtggat cgaacgcgtg ttgtattcgc
     2821 gcaggaacga atcggtctgc gcgcgcgtcc atttggccgg gatcggcgag accgcgtaga
     2881 aggtcttttg tgcagactcg agcgggcccg gggaatcgca gtacgccagc gccacgccct
     2941 gctggaactc cggcatgacg atgatctcga gtggttcgtc cggcacggtc acgatgttct
     3001 tgtcgcgcac gaactgcgtg gcatcgacca gcgtggcccg cgccgtatcg agcacggcat
     3061 cgcggtccgg acgttccttg tacgcgagct cgagcgccgc cttgatggcg cgctgctgtt
     3121 gcgacgcgct gggtttttcg ggcgtggacg gactgccgcg cttgcctttg agaacggtgg
     3181 ctgcgatctc gtacatggcg gtgcgcgtgg ccgccagttc gctctgcgct cgttcgcgga
     3241 tctcttcgcg tgaaagcggc gagaacaacg cgtacgcgag tttctgatcg tagaacgccg
     3301 cccctaaccg gaaatcaccc ttggcttccg gtgtgagccg cttgtcgagc cagatctggt
     3361 gttgcgacag tgcggtgcgc gcctttgcca cgctggcacg cagcgaggtc tgctcgtcgg
     3421 ccggcaatct ggcgatctct tcggcgatct gtccgtcgag catcgatatc agaccggcgt
     3481 tctgtttcgc cgcagtttca gcatgcacct tcggcacgcg cgcaggttcg agtacctcgc
     3541 gcacctgcgc gaggaaacgc ggcatctcgt cgagccgctt gcccaggttc ttcatgcgct
     3601 cgttcgccgg tgcgaactca cgcgccagca ggttgtagag ggcatcgccg gcgatgcgcg
     3661 tgtagatcag tggattccac cggaagtcct gcagcgtctg gtacttccac tggtcgtatt
     3721 gaagatcgtt gcgcaggagc gctgcatcca cctggttgcc gcgcgacagc ctggtgacgt
     3781 cgatggggcc gagcgccgct agataggcct ccgcgaatgc ctcgcgcgcc tgccagccgg
     3841 cgacgctgac gtcgtcgagc cggtcgtcga atcgatgatc gccgagcgcg gtcgctccca
     3901 ccggcgactg ttccgggcct tccttcaggt aacgctgcgc gagcgcttcg aattgctgat
     3961 cggccgtggg cggcggcggt ggaggcggcg ggggcggatc ggcgcgcggt ggcgcgggag
     4021 cgcaggcgca cagcaatatc gccgcgattc ccgctacgac agttccagaa accgtcttca
     4081 tcgcttagtc ctcgcgcttc gcgccgcaat ggtcaacgga gcgcgacgtt accagctaaa
     4141 gtgcgcaacc cccaacactc gcgtccttca cccgacgacc ttgatcagcc actccagatc
     4201 gcctggtcgc caggccctga gtcatcccga tttcgcgcgg tacgcgtacg gccgattcgc
     4261 tgcgacgctg gcgtggcaga tgatcgacgt cgtcctcggc taccaggtgt gggagctgac
     4321 gcggaaagtg gaatatctgg ggctcatcgg gcttgcccag ttcctgccgt tcgtcatcct
     4381 gctcctgccc ggcggacagg tcgccgatcg cttcgatcgc cggctcatca tctcgctcgc
     4441 ctacggactc gaactgatcg cggcggtggc attactgtgg ttcacgctct tcagccgcca
     4501 cgaagtcatc ctgctcttct tcatcgccgc cttgttaggc gtgggtcgca gcttctgggc
     4561 accggcgggc caggcgatgg tgcccaacct cgtgccgaag gaactgctgt ccggcgccat
     4621 ctccatgaac acgctgatgt tcaccatcgc gaccatcagc ggtccggcga tcgccggact
     4681 cacgctgatc ctcggcatcg actggtcgta cggaattgcg gtggcactcc tgatcgttgc
     4741 gatcttcatg atgctcggcg tgaagccggt gctcgccaag tcggtgtccg aatggcgctg
     4801 gggcgatgtg ctcggtgggt tcgtgttcgt atggcggaag aagcccgtgc tcggcgcaat
     4861 ctcgctggac ctgttcgccg tgttgttcgg tggagtcgtt gcgctgctgc cggcatacgc
     4921 ggacaagatt ctgcacgtcg ggcccgtggg cctcggaatc atgcgggccg cgcccggcgt
     4981 gggcgcggcc atcgtggcgt tctggctcgg catgcggccg atccagcggc acgcgggaac
     5041 ctggatgttc ggcggcgttg cgctgttcgg cgtctgcatg atcaccgtgg gcctgtcgac
     5101 cagcatctgg ctgacaatcg tggcgctgat catcgccggc gcgggcgaca tggtcagc
//
LOCUS       DATDEU010000524         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_540727, whole genome shotgun sequence.
ACCESSION   DATDEU010000524 DATDEU010000000
VERSION     DATDEU010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540727"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VIV63_12515"
     CDS             <1..890
                     /locus_tag="VIV63_12515"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY6125467.1"
                     /translation="LWRVEEPRSFARRVTEYVALLIVGPIVVVSFIGLSHNALETAQA
                     GITRYMPFFERVLAVGQKVSPYFMVAAIFTAVYMFVPNTKVKWKPALIGGLTAGILWA
                     AVGNVFTALVVYSTRLTVVYAGFAIIVAALLWTYFGWLILLIGAQLSFYVQNRNYLRM
                     GLTELRLSAVQREQLTLKVMFLIGRAYHDGKTRWSVDTLAHELAMPGIAISRIVHGLE
                     AARLVTLTDDERLLPARDLSQITIQEIVDISRNEKSGQVGWRNLKIPAVDAISKTMDD
                     AWRKSCGEMTLRDLIDDAA"
     gene            complement(891..1895)
                     /locus_tag="VIV63_12520"
     CDS             complement(891..1895)
                     /locus_tag="VIV63_12520"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEY6125468.1"
                     /translation="MSPGDPALLHIVNGDMAAGVFLHTFGAGDRLLINRDVLSCGPLP
                     RSIRVAAWQRARLDFWRATLAHLRDFNFEPSPIDLLKNADRLRAPDIPCVWAATGNSD
                     QLTISFVLHQVAAAGGDVSGVHVVQFETLPNSEQRVRGTGELSPEQLTVHPAPRKLSA
                     TELAAYRDAWVAVTSSDPSTVESFPVRHPNAPWYLRDAVSKMLRRYPDRASGLNYWDR
                     RLLFDVQSKGPRAASVLAHIVEAMFDDGDLVGDLYIASRILAMSNGSLPRPLLSFNGS
                     RQQVGRADVTLTEFGQEVTSGGASSYPVNPIDDWAGGVHVSSANGNLWFNDDGRIVRG
                     "
     gene            complement(1892..>2244)
                     /locus_tag="VIV63_12525"
     CDS             complement(1892..>2244)
                     /locus_tag="VIV63_12525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125469.1"
                     /translation="PSIPQVKGDYVQVQQVLLNLVMNAVEAVVDMPPERRRVSISAVA
                     QSPGQVEVFVRDEGHGIAADKLQRIFEPFFTTKVGGMGLGLSISRSIVQAHGGRIWAE
                     SDTTGTTFRFILPA"
BASE COUNT          420 a          745 c          682 g          397 t
ORIGIN      
        1 tcctctggcg tgtcgaagag ccgcgcagct tcgcgcggcg tgtcaccgaa tacgtggcgc
       61 tgctcatcgt gggccccatc gtcgtcgtga gtttcatcgg cctctcgcac aacgcgctcg
      121 aaaccgcgca ggcgggcatc acgcgctaca tgccgttctt cgaacgggtg ctcgccgtcg
      181 gccagaaagt ctcgccctac ttcatggtgg cggcgatctt caccgccgtg tacatgttcg
      241 tgcccaacac caaggtcaaa tggaagcccg cgctcatcgg cggcctcacc gccggcatcc
      301 tctgggccgc cgtgggcaac gtgttcaccg cgctcgtcgt gtactccacg cggctcacgg
      361 tggtgtatgc gggcttcgcg atcatcgtcg cggcgttgct gtggacctac ttcggctggc
      421 tgatcctgct catcggcgcg cagctgtcgt tctatgttca gaaccgcaac tacctgcgca
      481 tggggctcac ggaactacgc ctatcggcgg tccagcgcga gcagctcacg ctcaaagtca
      541 tgttcctgat cggccgtgcc tatcacgacg gcaagacgcg ctggtcggtg gacacactgg
      601 cgcacgaact cgccatgccg ggcatcgcca tctcgcgcat cgtgcacggg ctcgaggccg
      661 cacgtctggt gacgctcacc gacgacgaac gcctgttgcc cgcgcgcgat ctgagtcaga
      721 tcacgatcca ggaaatcgtc gacatctcgc gcaacgaaaa atccggccag gtcggctggc
      781 gcaatctcaa gattccggcg gtggatgcca tcagcaagac gatggatgac gcctggcgca
      841 agagctgcgg agaaatgacg cttcgggacc tcatcgacga cgcggcgtga tcagccgcgc
      901 acgatgcgcc cgtcgtcgtt gaaccagaga ttgccgttcg ccgacgacac gtgaacgccg
      961 cccgcccaat cgtcgatggg attgaccggg tatgacgatg caccaccgct cgttacctcc
     1021 tggccgaatt ccgtcagtgt cacgtcggcg cgtcccacct gctgacggct tccgttgaaa
     1081 ctcagcaatg ggcgaggcag cgagccattg ctcatcgcga gaatgcgcga tgcgatgtac
     1141 aggtcgccca ccaggtcacc gtcgtcgaac atggcttcga cgatgtgcgc gagaactgac
     1201 gccgcccgcg gacctttgct ctgcacgtcg aacaggagcc ttcgatccca gtagttgagg
     1261 cccgacgcgc gatccggata ccgccgcaac atcttgctga cggcgtcacg gagataccac
     1321 ggcgcgttcg gatggcggac aggaaatgac tccaccgtgg aagggtcgct ggacgtcacg
     1381 gcgacccagg catcccgata cgccgcgagc tcagtcgccg aaagcttccg cggtgccgga
     1441 tgcacggtca gttgctcggg cgacagctcg cccgtcccgc gaacacgctg ttccgaattc
     1501 gggagcgtct cgaactgcac gacgtgaaca ccactcacgt ctccgcccgc cgccgccacc
     1561 tggtggagca cgaatgagat cgtgagctga tcgctgttgc cggtcgccgc ccagacacag
     1621 ggaatatccg gagcccgcag gcgatcggca ttcttgagca gatcgatcgg tgacggctcg
     1681 aagttgaaat cacgaaggtg tgcgagcgtc gctcgccaga aatcgagccg cgccctttgc
     1741 caggccgcga cccggatgct ccgtggcagc ggcccgcagg acaggacgtc ccgattgatc
     1801 agcagtcgat cgcccgcgcc aaacgtgtgc aggaacacgc ctgcagccat gtctccgtta
     1861 acgatgtgga gaagtgcggg atcgccggga ctcacgccgg cagtatgaaa cgaaacgtcg
     1921 tccccgtggt gtcggactcc gcccagatac ggccgccatg cgcttgcacg atcgagcgcg
     1981 agatggacaa cccgagaccc atgcctccga ctttggtcgt gaagaacggc tcgaatatgc
     2041 gctgcaattt gtccgcggcg ataccatgac cctcatccct gacgaacact tcgacctggc
     2101 cgggcgattg cgcaaccgcc gagatcgaca cgcgccgccg ctcgggtggc atgtcgacga
     2161 ccgcctccac cgcgttcatg acgagattga gcagcacctg ctgcacctgt acgtagtcgc
     2221 ctttgacctg cggtatcgac ggat
//
LOCUS       DATDEU010000525         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_685261, whole genome shotgun sequence.
ACCESSION   DATDEU010000525 DATDEU010000000
VERSION     DATDEU010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685261"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VIV63_12530"
     CDS             <1..860
                     /locus_tag="VIV63_12530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6125470.1"
                     /translation="FRARDHRGSRDVVLKFPKPRPGLDPLLRAALLREMWIASHVRSP
                     FVTEALEPPTERRSCLYGVLPFYSGETLELRLRRQPPVPLAAGLSIALKLTKAVAALH
                     RAGIIHRDIKPENIMLETDGGLKLIDLGVARLRQFDEGESLEVPGTRSYMAPELFAGT
                     PADESADIFALGVTLYRMFTGGAYPYGEVEAFSSPRLGKATALAKLRPDLPAWLDRAI
                     ARAIAIDRRERHADAIEFGFELEHGSLRAIPHAMERQSLYDRHPIRFWQLVSVSLFVA
                     LLVALSHLD"
     gene            1039..2406
                     /locus_tag="VIV63_12535"
     CDS             1039..2406
                     /locus_tag="VIV63_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY6125471.1"
                     /translation="MRKSICAGAICALFAFTHVMAAGKGPEKEQLKLGFIKLTDMAPL
                     AIAYEKGYFEDEGLYVTLEPQANWKVILDRVITGELDGAHMLAGQPLGASVGIGTKAD
                     VVTGFSMDLNGNAITVSNEVWALMKAGLATDEKGLPKHPIPATALKPALEKFKASGKS
                     FKMAMVFPVSTHNYELRYWLAAGGISPGFYSPSDVTGQTRADILISVTPPPQMPATLE
                     AGTINGYSVGEPWNQQAIFKGVGVPVITDYEIWKNNPEKVFGVTKAFADKNPRTHIAL
                     IKALTRATIWLDEKNGANRKEAAQILSRPEYVGADYRVIANSMTGTFEYGRGDKRALP
                     DFNVFFRYHANYPFYSDAIWYLTQMRRWGQIPEGKPDAWYVETAKKVYLPEVFVAAAK
                     ELVAEKKANAADFDFLSDGFRAPTKDFIDGVEYDGRKPNAYIEKLALGLKGQQKIESG
                     NVVGK"
     gene            2390..>2854
                     /locus_tag="VIV63_12540"
     CDS             2390..>2854
                     /locus_tag="VIV63_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125472.1"
                     /translation="MSSASNVKAVDVVAVEPAVAPPARPGANPSAPKALFPRVNAGID
                     ATIRALSLVGLGFLQPIVSIMRGEDPRVHMRQLWLDLGAPVLAIAAFLLVWSQVSQGI
                     QTSLGQIPGPTAVWEQTKALWADHQVQREKAVAFYERQEKRNAEKQAEDPAAD"
BASE COUNT          545 a          895 c          912 g          502 t
ORIGIN      
        1 tgtttcgcgc gcgggatcac cgcggcagcc gtgatgtcgt gttgaagttt ccgaagccgc
       61 gcccggggct cgacccgctg ctgcgcgcgg cgctgctgcg cgagatgtgg atcgcgagtc
      121 atgtgcgcag cccgttcgtc accgaggcgc tggaaccgcc gaccgagcgc cgcagctgcc
      181 tttatggagt gctgcctttc tactcgggcg agacactcga actacgtctg cggcgtcagc
      241 cgccggtacc gctcgcggca ggactctcga tcgcgctgaa gctgaccaag gccgtggctg
      301 cgctgcatcg cgccggcatc attcatcgcg acatcaagcc cgagaacatc atgctcgaga
      361 ccgacggtgg gctgaagctc atcgacctgg gcgtggcgcg cctgcggcag ttcgacgaag
      421 gcgaatcgct cgaggtgccc ggcacgcgaa gttatatggc gccagaattg ttcgccggta
      481 ctccggcgga tgaaagcgct gacatcttcg cgctcggggt cacgctctat cgaatgttca
      541 cgggcggcgc ctatccctat ggagaagtag aggccttttc gagtccgcgg ctcggcaagg
      601 cgacggcgct cgccaagctc cggccggact tgccggcctg gctcgatcgc gccatcgcgc
      661 gcgccatcgc catcgaccgg cgcgagcgtc acgcggatgc gatcgagttc ggcttcgagc
      721 tcgagcacgg ttcgctgcgg gcgattccgc atgccatgga acgccagtcg ttatatgacc
      781 ggcatccgat tcgtttctgg caactggtgt ccgtgtcgtt gttcgtcgcg ctgctggttg
      841 cgctctcaca tctcgactga gtccacgcgc aaaaagtgca ccgcattgaa agcgcgcgtg
      901 cacccctccg ccgcgtttcg gtgcaggtcg ccgcaagcag ggccacggct gcagaaaaac
      961 acctaaaaga aacgctggca tgcagattgc tttgtcccgg ctgcgcgacc catagcgcga
     1021 cgggagaggt agtcagtagt gagaaaaagt atttgtgccg gcgccatctg cgcgttgttc
     1081 gctttcaccc atgtgatggc cgcgggcaaa ggtccggaga aagagcagct caagctgggt
     1141 ttcatcaaac tcaccgacat ggcgccgctc gccattgcct atgaaaaagg ctacttcgaa
     1201 gacgaaggcc tgtacgtcac gctcgagccg caggcgaact ggaaagtcat tctcgatcgc
     1261 gtgatcaccg gtgagctcga tggcgcgcac atgctcgcgg gccagccgct cggcgcttcg
     1321 gtgggaatcg gcaccaaggc cgacgtggtc accggcttca gcatggatct caacggcaac
     1381 gccatcacgg tctccaacga agtgtgggcg ctgatgaaag ccggccttgc taccgacgag
     1441 aagggtctgc ccaaacatcc gattcctgcc accgcgttga agcctgcgct ggagaagttc
     1501 aaggcctcgg gcaagtcgtt caagatggcc atggtttttc ccgtgtccac tcacaactac
     1561 gagttgcgct actggctggc cgccggtggc atcagccccg gtttctattc gccctccgac
     1621 gtcacgggac agacgcgcgc cgacatcctg atttcggtga cgccgccgcc gcagatgccg
     1681 gccacgctcg aagccgggac gatcaacgga tattcggtcg gcgagccctg gaaccagcag
     1741 gcgatcttca agggcgtcgg cgtgccggtc atcaccgact acgaaatctg gaagaacaat
     1801 ccggagaagg tcttcggcgt caccaaggca ttcgccgaca agaatccgcg cacgcacatt
     1861 gcgctgatca aggcattgac gcgcgccacg atctggctcg acgagaagaa tggcgcaaac
     1921 cgcaaggaag cggcgcagat cctctcgcgt cccgaatacg tcggtgccga ctatcgcgtc
     1981 atcgccaaca gcatgacggg caccttcgaa tacgggcgcg gtgacaagcg cgcgctgccg
     2041 gacttcaacg tgttcttccg gtatcacgcc aactatccgt tctattcgga tgccatctgg
     2101 tacctgacgc agatgcgccg ctggggtcag atccccgagg gcaagcccga cgcgtggtac
     2161 gtcgaaaccg cgaagaaggt ctatctgccg gaagtgttcg tcgcggccgc gaaggaactg
     2221 gttgccgaga agaaggccaa cgccgccgac ttcgatttcc tgagcgatgg cttccgcgca
     2281 ccgacgaagg atttcatcga cggcgtcgaa tatgacggcc gcaagccgaa tgcgtacatc
     2341 gagaagctgg cacttggcct caagggtcag cagaagatcg agagtggcaa tgtcgtcggc
     2401 aagtaacgtg aaggccgtgg acgtggtggc ggtggagccc gcagtggcgc caccggccag
     2461 gccgggagcc aatccgtccg cgccgaaggc gctgttcccg cgcgtgaatg cgggcatcga
     2521 cgccaccatc cgcgcgctct cgctcgtggg cctcggattt ctccagccca tcgtctccat
     2581 catgcgcggc gaggatccgc gagtgcacat gcgccagctc tggctggatc tcggcgcacc
     2641 cgtgctggcc atcgcggcgt ttctgctggt gtggagccag gtgtcgcagg gcattcaaac
     2701 gagcctcggg cagattccgg gtcccaccgc ggtgtgggag cagaccaagg cgctgtgggc
     2761 cgaccaccag gtgcagcgcg agaaagccgt ggcgttctac gagcgccagg aaaagcgcaa
     2821 cgccgagaag caggcggaag atccggccgc ggac
//
LOCUS       DATDEU010000526         3434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1998529, whole genome shotgun sequence.
ACCESSION   DATDEU010000526 DATDEU010000000
VERSION     DATDEU010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998529"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VIV63_12545"
     CDS             complement(<1..1043)
                     /locus_tag="VIV63_12545"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY6125473.1"
                     /translation="MNVLRMADQDLAGKRVLIREDLNVPVEGGRVTSDARIRASLPTI
                     KLALSKGAKVFIASHLGRPEEGVYAEEFSLAPVAARLSELLGQPVPLKKDWLAGVDCP
                     AGSAVLLENVRFNKGEKKDKEDLAKQMASLCDVFVMDAFGTAHRAEASTHGVGKFAKI
                     ASAGPLLVGELEALERALAKPARPLVAIVAGSKVSTKLTVLESLLSKVDKLIVGGGIA
                     NTFLAATGINVGKSLQEAEMHDIARKLLAQAKERGAEIPLPTDVVVAKEFAATAHADV
                     RSIHDVRPDEMILDIGPDTAERLGALLQKAGTIIWNGPVGVFEFDQFGEGTKTIAQAI
                     AKSPAFSLAGGGDT"
     gene            complement(1055..2068)
                     /gene="gap"
                     /locus_tag="VIV63_12550"
     CDS             complement(1055..2068)
                     /gene="gap"
                     /locus_tag="VIV63_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY6125474.1"
                     /translation="MAIKVGINGYGRIGRNILRALYEAKRNNEIQIVAVNDLGDAQTN
                     AHLTQYDTAHGKFPGTVSVEGDNIVVNGDKIKVFAQRDPSKIDWASVGAEYVFESTGF
                     FTTKADAGKHLAGGAKKVVISAPGGKDVDATVVYGVNHNVLKKEHTVISNASCTTNCL
                     APLAKVLNDKIGIVSGLMTTIHAYTNDQRLTDVYHEDIRRARSATMSMIPAKTGAAAA
                     VGLVLPELKGKLDGFAIRIPTINVSLVDLTFTAKRATTVEEINKLVKEAAEGAMKGVL
                     AYTEKQLVSIDYNHDPASSTYDATLTRVTEGTLVKVCSWYDNEWGFSNRMLDTTIAWS
                     KAS"
     gene            complement(2121..>3434)
                     /locus_tag="VIV63_12555"
     CDS             complement(2121..>3434)
                     /locus_tag="VIV63_12555"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY6125475.1"
                     /translation="KKAKNRKDQPSIICCKTIIGWGAPNKQGTKSAHGEALGPDEVAN
                     ARKTLGWESPPFVIPEDIRAAWDHKEEGAAAEKKWLRVWRRYKREFPTEGAEFERRMK
                     GDLPADWAEIVQSAIAAGAAVTGAQATRASSQAALNVLGPRMPEMLGGSADLTGSVNT
                     KRKDSVDLTVDNFKGNYVYYGVREFGMTAIMNGLTVHGGYRPYGGTFLVFSDYARNAV
                     RVAAIMHAPVTLVYTHDSIGLGEDGPTHQPIEHLASLRAMPNLHTWRPCDAVESAVSW
                     AAALERKNGPTALIFTRQGLPQHPRTPEQLVALRKGGYVLIDSNGPPEAIVIATGSEV
                     GFAAEAVKELQAAGKRVRLVSMPCCEAFDAQPAPYRESVLPASCTRRLAVEAGSTQSW
                     WRYVGSQGRVMGIESFGASGKGPDLFKHFGLTAADLKAQVEQLLG"
BASE COUNT          567 a         1123 c         1080 g          664 t
ORIGIN      
        1 gtgtcgccgc cgccagccag cgaaaacgcc ggtgacttcg cgatcgcctg ggcaatggtc
       61 ttcgtgcctt cgccaaactg atcgaactcg aacacgccca cgggaccatt ccagatgatc
      121 gtgccggcct tctgcagcaa cgcgcccagg cgctctgcgg tatcgggacc gatgtcgagg
      181 atcatctcgt ccggacggac gtcgtgaatg ctgcgcacgt cggcatgcgc cgtggccgcg
      241 aattccttcg cgacgacgac atccgtcggc aacgggattt ccgcgccgcg ctccttggcc
      301 tgcgccagga gtttgcgcgc gatgtcgtgc atctccgctt cctgcagcga cttgcccacg
      361 ttgatacccg tggcggcgag gaacgtgttg gcgatgccac cacccacgat cagcttgtcc
      421 accttgctca acagcgattc gagcacggtc aacttggtcg acactttcga gccagcgaca
      481 atagccacga gcggcctggc gggcttcgcc agcgcgcgct cgagcgcttc gagctcaccc
      541 acgagcaacg ggcctgcgct cgcaatcttg gcgaacttgc cgacgccatg cgtcgaagct
      601 tccgcgcgat gggccgtacc gaacgcgtcc atgacgaaca cgtcgcacaa cgacgccatc
      661 tgcttcgcga gatcttcctt gtctttcttc tcgcccttgt tgaagcgcac gttctcgagc
      721 agcaccgcgc tgcccgccgg gcaatcgacg cccgcgagcc agtctttctt cagcgggacc
      781 ggctggccta acaactcgga cagacgcgcg gcgacgggag ccagcgagaa ctcctcggcg
      841 tacacgccct cttccggccg gccgaggtgc gaggcgatga acaccttcgc gcccttggac
      901 agcgccagct tgatggtggg caacgacgcg cggatacgcg cgtcgctggt gacccggccg
      961 ccctcgacgg gcacgttcaa atcttcgcga atcaggaccc gcttgccagc gaggtcctgg
     1021 tccgccatgc gcaacacgtt catggattac ctcctcagga ggctttcgac caggcgatcg
     1081 tggtgtccag catgcggttc gagaagcccc actcgttgtc gtaccaggag cagaccttga
     1141 ccagcgtgcc ttcggtaaca cgcgtcaggg tcgcgtcgta ggtcgacgag gccgggtcgt
     1201 ggttgtagtc gatcgagacg agctgctttt cggtgtacgc cagcacgccc ttcatggcgc
     1261 cttcggcggc ttccttcacg agcttgttga tctcttcgac ggtcgtcgcg cgcttcgcgg
     1321 tgaacgtcag gtcgaccagc gacacgttga tggtcgggat gcggatcgcg aagccatcga
     1381 gcttgccctt gagttcgggc agcacgaggc ccacggccgc ggcggcgccg gtcttggcgg
     1441 ggatcatcga catcgtggcc gagcgcgcac gacggatgtc ctcgtgatag acgtcggtca
     1501 ggcgctggtc gttggtgtac gcatggatcg tcgtcatcaa accactgacg atgccgatct
     1561 tgtcgttgag caccttcgcg agcggcgcga ggcagttcgt ggtgcacgag gcgttcgaga
     1621 tgaccgtgtg ctctttcttg agcacgttgt ggttcacgcc gtagacgacc gtcgcgtcca
     1681 cgtccttgcc gcccggggcg gagatgacca ccttcttcgc gccgccagcc aggtgcttgc
     1741 ccgcatccgc cttggtggtg aaaaagccgg tggactcgaa cacgtattcc gcgccgaccg
     1801 aggcccagtc gatcttcgac gggtcacgct gtgcgaacac tttgatcttg tcgccgttga
     1861 cgacgatgtt gtcgccttcg acgctcaccg tgccggggaa tttgccgtgc gcagtgtcgt
     1921 actgcgtgag gtgcgcattg gtctgcgcat cgccgaggtc gttgaccgcg acgatctgaa
     1981 tttcgttgtt gcgcttcgct tcgtagagcg cgcgcaggat gttgcggccg atacgcccgt
     2041 aaccgttgat acctactttg atggccatct gtcccaatcc ccttacgttg aaaacttcac
     2101 gaaagaatct gtggtcgact tcagccgagc agttgctcga cctgcgcctt gaggtcggcg
     2161 gcggtcagac cgaaatgctt gaacaggtcc gggcccttgc ccgaggcgcc gaacgattcg
     2221 atgcccatga cgcgaccctg cgagccgacg tagcgccacc acgactgcgt ggagcccgct
     2281 tcgaccgcga ggcggcgcgt gcacgaggcc ggcaacacgc tctcgcgata cggcgcaggt
     2341 tgcgcgtcga aggcttcgca gcaaggcatg gacaccaggc gcacgcgttt gcccgcggcc
     2401 tgcagttcct tcacggcctc ggccgcgaat cccacttccg aacccgtggc gatcacgatg
     2461 gcttcgggcg gaccgttgct gtcgatgagc acataaccac ccttgcgcag cgcgaccagc
     2521 tgctcgggcg tgcgcggatg ctgcggcaga ccctggcgcg tgaagatgag cgccgtcgga
     2581 ccgttcttgc gttcgagcgc cgccgcccac gacaccgccg actccaccgc atcgcaggga
     2641 cgccaggtgt gcaggttggg catggcgcga aggctcgcga gatgctcgat gggttgatgc
     2701 gtcgggccgt cttcgccgag accgatggaa tcgtgcgtat aaaccagcgt gaccggtgcg
     2761 tgcatgatcg ccgcgacgcg gaccgcgttg cgcgcgtaat cggagaacac gaggaacgtg
     2821 ccgccatacg gacgatagcc gccgtgcacg gtgaggccgt tcatgatggc cgtcatgccg
     2881 aactcacgaa caccgtaata aacgtagttg cccttgaagt tgtcgacggt gaggtcgaca
     2941 gaatccttgc gcttggtgtt caccgaaccg gtgaggtcgg ccgaaccacc cagcatttcg
     3001 ggcatcctgg gaccgagcac gttgagcgcc gcctgtgacg acgcccgcgt ggcctgcgca
     3061 ccggtgaccg ccgcgccagc ggcaatggcc gactgcacga tctcggccca gtcggccggc
     3121 agatcgccct tcatgcggcg ctcgaactcc gcgccttcgg tcgggaattc acgcttgtag
     3181 cggcgccata cgcgcagcca cttcttttcc gcggcggcgc cttcttcctt gtggtcccag
     3241 gcggcacgaa tgtcttccgg aatcacgaac ggcggcgatt cccagccgag cgtcttgcgc
     3301 gcattcgcga cttcatcggg acccagcgct tcgccgtgtg ccgacttggt gccctgtttg
     3361 ttaggagcgc cccagccgat gatggtcttg cagcagatga tcgaaggctg atccttgcgg
     3421 ttcttcgcct tctt
//
LOCUS       DATDEU010000527        10541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1124939, whole genome shotgun sequence.
ACCESSION   DATDEU010000527 DATDEU010000000
VERSION     DATDEU010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124939"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="epmB"
                     /locus_tag="VIV63_12560"
     CDS             complement(<1..880)
                     /gene="epmB"
                     /locus_tag="VIV63_12560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HEY6125476.1"
                     /translation="MTASLSTWQRELSRSITDPEQLLAELDLDPALGAPARAASSGFS
                     LRVTRSYLSRMRRGDARDPLLRQVLPLADELLETPGFEADPLQEHAATRAPGLLHKYA
                     GRALLITTQACAIHCRYCFRREFPYSAQQEAAEEGATRFSAALEVIAGDTTLEEIILS
                     GGDPLSLSDARLTRITDALAGMRHLQRLRVHTRQPVVLPSRVDEGLLTWLRGIRLPTV
                     FVLHTNHPNEIDGDVRAACTKLRSAGVTLLNQSVLLEGVNDDVDTLAQLSRALMDAGV
                     VPYYLHLPDRVRGTAHF"
     gene            complement(877..1683)
                     /locus_tag="VIV63_12565"
     CDS             complement(877..1683)
                     /locus_tag="VIV63_12565"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY6125477.1"
                     /translation="MHSKLVLIMSLAFAAPAFSQALNPVGDAKAAAPGEWHSLFNGKD
                     LSGWTPKINKHPLGVNYANTFRVEDGVIKVSYDGYDKFDSQFGHLYTNQAYGNYILQL
                     EYKITGAAIADSPPWAKLNSGVMIHSQSPLSMGLNQAFPASMEFQFLAVGATAGKQSG
                     NVCTPGTNLEMKGKLLTDHIIDSSSKLFPVDEWVAVEVEVRGNQEVIHRINGVEVLRY
                     QHPQMDPKDESGLALLTAGAPLQLSFGHIALQAESQPIWFRNIRIRQLQP"
     gene            1806..2339
                     /locus_tag="VIV63_12570"
     CDS             1806..2339
                     /locus_tag="VIV63_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125478.1"
                     /translation="MVTFKLRIAILVASCFAAGNVLAQSSETPSAAPAPAPMSEMPVY
                     DTALAPGWQNWSWAKADLSVELTGSARKPIRVVAGPYQALYLHHDPFSTTGMSKLSLL
                     IQGSAPDGEVRIFALTDGKPVGEGKHLKIGNTGWTQVVVPLATLGVEDKTIDGLWVQN
                     ATGADLPKFYVTEIRIH"
     gene            2426..3820
                     /locus_tag="VIV63_12575"
     CDS             2426..3820
                     /locus_tag="VIV63_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125479.1"
                     /translation="MILKYAGRHALKRNPWVLHQAADLASREFARPAEHHARQERRLE
                     RVFRDARNIPAYARHVAKVSSASRLASLSDVFPIIDKATLLRDRADFFAGGRIPKYRS
                     TVAATSGTTGTPLDVYRSLASILWEEAFHLQHWQWAGWNRGQRQAVLRGEIVIPTGQR
                     NPPYWFKDTVGGQLILSTRHLDRRTACLFADELLRFGATQLRAYPSAAYELATLVAET
                     GIPVRFQSVITGSEMLYDFQRQRIESVFKTRVFDFYSMAERVAYASQCERGRMHVNPE
                     YGVLEIVDEHGLPTDGEGSIVGTSLHNAVAPLIRYRMNDTARWNHEPCPCGRTYPVIE
                     NIGGRLADQLYDLDGRAVNGTVIGFAFDGMRHIQKAQVVQPADDHWLIRIVPGRGYSA
                     ADGQQVLDKLAAEVSSRVVARIEVVDSISALPSGKYKWVVREYGHEMPSPGAPGITSR
                     PPPRVAGTGPGALC"
     gene            3814..4782
                     /locus_tag="VIV63_12580"
     CDS             3814..4782
                     /locus_tag="VIV63_12580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6125480.1"
                     /translation="MLNELTMDDRSRDPAVIAESVTARDRWDRLLAQVSLPHFTQSWC
                     YGEGKRSQGWAVERLQFAYAGEAVAICQVLVKRVLGLPVAARINRGPLFLGEASPEVQ
                     LTVLQALRQRWRYGRRGLLLMAPSLPFDAASTSLLRAAGFTSRKPGGWGSALIDLHPS
                     LDAIRAGLSSKWRNHLNGSLKAGIAVRVSGEREVFDWMLEQHAGNMAIKGYVGPSVSF
                     VRNMIASSPQDFSVYQAYIDNQPVSAILLARFGTHAETYLSWTGEAGRRSNAHHLLLW
                     QLIVESKANGCRAIDLGGYTTSGKYGAYKRGMKGREYRLTGEWIAF"
     gene            complement(4779..6284)
                     /locus_tag="VIV63_12585"
     CDS             complement(4779..6284)
                     /locus_tag="VIV63_12585"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY6125481.1"
                     /translation="MNRLSISIAAGLLAFAPRLQACAEHAMVGSLDLTTATVPDLRAA
                     LENKKTTSVLLVQGYLARIADCNDTLHAVIATHAHALRDAAALDRERKAGKIRGPLHG
                     IPVVIKDNIDLADSVTTAGSLALAKNLRTRNAPLVDRLNDAGVIVIAKANLSEWANFR
                     SRWSSSGWSAVGGLAVNARNPQRSACGSSSGSAVAIAARLAPLAVGTETNGSIVCPSS
                     VNGVVGLKPTVGLIPGEGIVPISHSQDTAGPMTASVMDAALLLDAMADPKAAGKTNYS
                     SIRAGALKGARLGVARFIKGYSPATEALFNESLAVLKAQGAELVEIEQFDLAPLRDLQ
                     LPILLTEFKADLNAYLATTPASVESRTLADLMTFNRAEPREMAWFGQDLFEQAQATAG
                     LSDPAYLQTLQKARALAGPDGIDRLLKEHKVVALLAPTSGPAWSIDLINGDRSVGSAS
                     LLAAIAGYPHLTVPMGDVAGMPVGLSLFGAAWSEQTLLSLGYSFELARTKR"
     gene            complement(6281..7084)
                     /locus_tag="VIV63_12590"
     CDS             complement(6281..7084)
                     /locus_tag="VIV63_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125482.1"
                     /translation="MTAVLFGIAGATVAQEGRNPLNATAVVDMGGFFLSTDIQVRLDG
                     EGGRVGDTIDFDDTFGLDRFERFRVDGLWRIKGKHSIRGTYFKNNRSSTNELDRDINF
                     GDETYPVGATTTARWELEVMQLSYDYAFKRTDKYELAAGIGVHVLDARLALDATVTGA
                     GGSFSTELSESGSTDAPLPVLGLRGIWALPHNFYFTAQAQYFYVDYNEYNGSLSDLKA
                     TIVWQATPRFGVGLGYNDFRFRFDLEKNLFSGRLRWNYGGAMAFASVTF"
     gene            7270..8253
                     /locus_tag="VIV63_12595"
     CDS             7270..8253
                     /locus_tag="VIV63_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEY6125483.1"
                     /translation="MKRRSLLSAAAGAMVLAPQIHGSRAIAAEPGRAKELYGLLGKLP
                     PRDRKVGAQLVATEDRGSYTLETLVLDLNGIESAPAYFAKPKGKTGRLPTVLFNHSHG
                     GGYKIGKTEFVEGREYLNNPPYAEFLTSLGFNALCFDAWIFGERARKTELETFKDMLW
                     HGQVLWGMMVYDSLKAVDYLVTRPDVDTKRLGTVGMSMGSSTAQWAGALDARLKVVVD
                     ICCLTDWHTLVEVDGLKGHGIYYYVPDLLNHFTTAQMNALIAPRAHLSLAGNQDKLTP
                     LAGLEKIDAELKKVYASAGHPQNWKLLRYDGGHLENPQMREEIRRWFVEKL"
     gene            complement(8264..8701)
                     /locus_tag="VIV63_12600"
     CDS             complement(8264..8701)
                     /locus_tag="VIV63_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125484.1"
                     /translation="MARLLLALLLLPLTASAADEGRFSFLEQEVRNLQRQVQVLSRQM
                     DEMRLRPDRIDPQLSGPRPARGASPETNLPRWVDASTWKKIRPGMSELEVISSLGPPT
                     SMREEGGSRVLLYAMEIGPTAFLGGSVAIRNRAVVEVNQPALQ"
     gene            complement(8761..10410)
                     /gene="treF"
                     /locus_tag="VIV63_12605"
     CDS             complement(8761..10410)
                     /gene="treF"
                     /locus_tag="VIV63_12605"
                     /inference="COORDINATES: protein motif:HMM:NF009774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HEY6125485.1"
                     /translation="MCSSVPSIRARVLPAIAALLLVWAGVVAQSRDAAPRPPSQLYGE
                     LFHRVQTGKLFADSKTFADAVARTAPAKIVRDYEKSRNGEGFSLDRFVAAHFDVPSVA
                     GNDYQTTPRQEIRVHINQLWNALVRAPKGLPDGGSLLRLSTRYVVPGGRFRELYYWDS
                     YFTMLGLQVSGREDLIDDMVENFAGLIDRFGHIPNGSRTYYLSRSQPPFFAAMVELQA
                     EHDSRAMIRRLPQLEREYEFWMEGAASLTPGTAHRRVVRLDDGSLLNRYWDDLAIPRD
                     ESYLEDLETAKGSTRAAPDVYRDLRAAAESGWDFSSRWLADGKTLSTIHTTDFLPVDL
                     NSLLYKLELTIARGCEEAKRPDCDTRMRANALARQTAMLRVMWSRELDAFTDYDWRNK
                     VTSTRLTAATVFPLYVGLASSQQAQRVASTLRESLLTPHGLATTTETSGQQWDAPNGW
                     APLQWIAIEGLRKNGDPDLAAKIAEGWVAENARVYCKTGKLVEKYNVRDAGEGSGGEY
                     PVQDGFGWTNGVLLKLLAVYPQLSARRYEFTGAACATTSIH"
BASE COUNT         1954 a         3368 c         3329 g         1890 t
ORIGIN      
        1 cgaagtgcgc ggttccgcgc acgcgatccg gcaggtgcag gtagtaaggc accactccgg
       61 cgtccatcaa cgcgcgtgac aactgcgcga gggtgtccac gtcgtcgttg acaccctcga
      121 gcagcacgga ctggttgagc agcgtcacgc ctgccgaacg cagcttcgtg catgcggcgc
      181 gcacgtcacc gtcgatctcg ttgggatgat tggtgtgcag cacgaacacc gtgggcagcc
      241 tgatgccgcg caaccacgtg agcaatcctt cgtccacgcg cgacggcagc accaccggct
      301 gccgcgtatg cacgcgcagg cgctgcaggt ggcgcatgcc agcgagtgca tcggtgatgc
      361 gcgtgagccg cgcgtcgctc aacgacagcg gatcgccgcc gctcaggatg atttcttcga
      421 gcgtggtatc gccggcgatc acctcgagcg cggcgctgaa tcgcgtggcg ccttcctcgg
      481 cggcttcctg ttgcgccgag tacggaaact cacgccggaa acagtagcgg caatggatcg
      541 cgcaggcctg ggtggtgatg agcagcgcgc gtcccgcgta tttgtgcagc aggcccggcg
      601 cgcgggttgc cgcgtgttcc tgcagcggat cggcctcgaa cccgggcgtc tccaggagct
      661 catcggcaag cggcaacacc tggcgcaaca agggatcgcg tgcatcgccg cgccgcatgc
      721 gggagagata actgcgcgtc acgcgcaaag aaaaacccga cgaggccgct cgcgccggcg
      781 cgcccagcgc cggatccagg tcgagctccg ccagcaattg ctccggatca gtgatcgacc
      841 gcgacagctc gcgttgccag gtagatagag aagccgtcat ggttggagct gacggatcct
      901 gatgttgcgg aaccagatgg gctgcgattc cgcctgcaac gcgatgtgcc cgaaggacaa
      961 ctgcaacggc gcgcccgcgg tgagcagcgc cagcccgctt tcgtccttgg gatccatctg
     1021 cgggtgctgg taacgcagca cttcgacgcc gttgatgcga tggatcactt cctggttgcc
     1081 gcgcacctcg acttccacgg ccacccactc gtccaccggg aacagcttcg acgacgagtc
     1141 gatgatgtga tccgtcagca atttgccctt catctcgaga ttcgtacccg gtgtgcagac
     1201 attgccgctc tgcttgcccg cggtggcgcc cacggccagg aactggaatt ccatgctcgc
     1261 tggaaacgcc tggttcaacc ccatgctcaa gggcgactgc gaatggatca tcacaccgct
     1321 attgagcttg gcccagggcg gcgagtcggc gatggccgcc ccggtgatct tgtactcgag
     1381 ctgcaggatg taatttccgt acgcctggtt ggtgtacaga tggccgaact ggctgtcgaa
     1441 cttgtcgtag ccgtcgtagc tcaccttgat gacgccgtct tcgacgcgaa acgtgttcgc
     1501 gtaattaacg ccgagcgggt gtttgttgat cttgggtgtc cagccggaga gatccttgcc
     1561 gttgaacagc gaatgccatt caccgggtgc ggcggctttg gcatcaccca ccggattcag
     1621 ggcttgtgag aacgcgggcg cggcgaaggc cagcgacata atcaggacga gtttcgaatg
     1681 catggttccc cctcggcgag gcgcgggagc ttatcagccg ttagcggtac cggcactcgc
     1741 cgtcgttcca tggcggtagt aacctttcgc ccgcaaaaaa caaaaccacc aattcggggg
     1801 aaaacgtggt cactttcaaa cttcgtatcg cgattctcgt cgcgtcatgc ttcgccgcgg
     1861 gcaatgtgct ggcacagtcc tcggagacgc cttccgccgc gcccgcgccg gcgcccatgt
     1921 cggaaatgcc ggtgtatgac accgcgctgg cgccgggatg gcagaactgg agctgggcca
     1981 aggccgacct cagtgtcgaa ctcaccggaa gcgcccggaa acccatccgc gtcgtggcgg
     2041 gtccctacca ggcgctctac ctgcatcacg accccttcag cacgacgggc atgtcgaaac
     2101 tctcgctgtt gatccagggt tcggcaccgg acggcgaagt gcgcatattc gcgttgaccg
     2161 acggcaagcc ggtcggcgaa ggcaaacacc tcaagatcgg caatacgggc tggacgcagg
     2221 tcgtcgtgcc gctggccacc ctgggcgtcg aggacaagac gatcgacggc ctgtgggtgc
     2281 aaaacgccac tggcgccgac ctgccgaagt tctacgtcac cgagatcagg atccactgac
     2341 acccggcgcg gcatccacct acgccgccca ccgcccgagg ttccgataat cggcgaccag
     2401 gaagaagcgc agggtggatg ttgcggtgat tctcaaatat gcgggccgcc atgcgctcaa
     2461 gcggaacccg tgggtgttgc accaggctgc ggatctggcg agccgcgagt ttgcgcgccc
     2521 ggccgaacac cacgcgcgtc aggagcgcag gctcgagcgc gtgtttcgcg acgcgcgcaa
     2581 tatccccgcg tacgcgcggc acgttgcaaa ggtaagcagc gcgagtcggc tcgcctcact
     2641 gagcgatgtc tttccgatca tcgacaaggc cacgcttctg cgtgaccgcg ccgacttctt
     2701 cgcgggcgga agaattccga aataccggtc gaccgtcgcg gccaccagcg gcacgacggg
     2761 cactccactg gacgtctacc ggagcctcgc ctccattctc tgggaggaag catttcacct
     2821 gcagcactgg cagtgggcgg gctggaatcg cggacagcgc caggcagtgc ttcgcggcga
     2881 gatcgtgatt cccaccggcc agcgcaatcc gccctattgg ttcaaggaca cggtcggtgg
     2941 tcaattgatc ctgtccacac gccacctcga tcgccgcacc gcgtgcctgt tcgccgatga
     3001 acttctccga ttcggcgcga cgcaattgcg cgcctatcca tccgctgcgt acgaactcgc
     3061 gacgctggtc gccgagaccg ggataccggt tcgattccag tccgtgatca ccggttccga
     3121 aatgctctac gacttccagc ggcagcgcat cgagtcggtc ttcaagaccc gcgtcttcga
     3181 cttctacagc atggcggaac gtgtcgcgta tgcctcgcag tgcgaacgcg gccgcatgca
     3241 tgtgaacccc gaatatggcg tgctggaaat cgtcgacgaa cacggactac ctaccgacgg
     3301 cgaaggttcg atcgtcggca cctctctgca caatgccgtg gcgccgctga tccgttaccg
     3361 gatgaacgac accgcacgct ggaatcacga gccctgcccc tgcggacgga cttatcccgt
     3421 catcgaaaac atcggtggcc ggctcgcgga ccagctctac gatctcgacg gccgcgccgt
     3481 gaatggcacc gtcatcggtt tcgcattcga cggcatgcgc cacatccaga aggcgcaggt
     3541 ggtgcagccc gccgacgatc actggctcat tcgcatcgtg cccggtcgcg gatattcggc
     3601 cgccgatgga cagcaggtgc tcgacaaact cgcggcagaa gtctcgtcac gcgtggtggc
     3661 gcgcatcgaa gtcgtcgaca gcatctcggc gctgccgagc ggcaagtaca agtgggtggt
     3721 tcgcgaatac ggccacgaaa tgccttcgcc tggagcaccg ggcatcactt cgcgcccgcc
     3781 tccgcgcgtc gcgggaacgg gaccgggtgc cctgtgttga acgaactcac catggacgat
     3841 cgatcgcgcg atcccgccgt cattgcggag tccgtgaccg cccgcgatcg gtgggatcgc
     3901 ctgctcgcgc aggtttcgct gccacatttc acgcaatcgt ggtgctacgg cgaaggcaag
     3961 cgcagccagg gctgggctgt cgagcgcctc cagttcgcct acgccggcga ggccgtcgct
     4021 atttgccagg ttctggtgaa gcgcgtgctc ggattgccgg tggcggcgcg catcaaccgc
     4081 ggaccactgt tcctcggcga ggcttccccg gaggttcagc tcacggtgct gcaggcgctg
     4141 cggcagcggt ggcgatatgg caggcgtgga ctgttgctca tggcgccatc cctgcccttc
     4201 gacgcggcga gtacgtcgct gcttcgcgcc gcggggttca cgtctcgcaa gcctggcggc
     4261 tggggctccg cgctgatcga ccttcaccct tcactcgacg cgattcgtgc aggcctgtct
     4321 tccaagtggc gcaatcatct caacggttcg ttgaaggcag gcatcgccgt gcgagtcagc
     4381 ggcgaacgcg aagtcttcga ctggatgctc gagcagcacg ccggcaacat ggcgatcaag
     4441 ggctacgtcg ggccgtcggt gtccttcgtc cgcaacatga tcgcctcgag cccgcaggat
     4501 ttttccgtct accaggcgta catcgacaac cagccggtct ccgccatcct gctggcgcgt
     4561 tttggcacgc acgcggagac gtatttgagc tggacgggag aagccggccg acgcagcaac
     4621 gcccatcacc tgctgttgtg gcagctcatc gtcgaatcga aggccaacgg atgccgcgcg
     4681 atcgatctgg gcggttacac caccagcggc aaatacggcg cctacaaacg cggcatgaag
     4741 ggacgcgagt accggctgac cggcgagtgg attgcgttct agcgcttcgt acgcgcgagc
     4801 tcgaacgaat accccagcga caacagtgtt tgttcgctcc acgcggcgcc gaacagtgaa
     4861 agccccacgg gcattcccgc cacgtcgccc atcggcacgg tgaggtgtgg atagccggca
     4921 atcgcggcca gcaacgacgc cgatcccacc gatcgatcgc cattgatgag atcgatggac
     4981 cacgcgggcc ccgatgtcgg tgccagcagc gcaaccacct tgtgctcttt gaggaggcgg
     5041 tcgattccat cgggaccagc cagagcgcgt gccttctgca gcgtctgcag ataagccggg
     5101 tcgctcaagc ccgcggtcgc ctgcgcctgc tcgaagagat cctggccgaa ccacgccatc
     5161 tcgcgcggct cggcgcggtt gaacgtcatg agatcggcga gcgtgcgcga ctccaccgag
     5221 gccggcgtag tcgccagata ggcgttgaga tcggccttga actccgtgag caggatcggg
     5281 agctgcagat cgcgcagcgg cgcgaggtcg aactgttcga tctccacgag ttccgcgccc
     5341 tgcgctttca acaccgccag cgattcgttg aacaacgctt cggtggccgg tgagtaaccc
     5401 ttgatgaaac gcgccactcc cagccgcgcg cccttcagcg cgccggccct gatgctgctg
     5461 tagttagtct tgccggcggc tttcgggtcc gccatggcgt cgagcagcag cgcggcgtcc
     5521 atgacgctcg ccgtcatggg gcccgccgtg tcctgcgagt ggctgatggg cacgatgccc
     5581 tcgcccggga tcaacccaac cgtcggcttc aagcccacca cgccattgac ggaggacgga
     5641 cagacgatgg agccgttggt ctcggttccc accgcgagcg gcgcgagccg cgccgcaatc
     5701 gcgaccgcgg aacccgagct cgagccgcag gccgagcgct gcggatttcg cgcattgacg
     5761 gccagcccgc ccacggcgct ccagccgctc gacgaccagc gcgaacggaa attggcccac
     5821 tccgacagat tggccttggc gatgacgatg acgcccgcgt cgttgagccg gtcgaccagt
     5881 ggcgcgttgc gcgtgcgcag gttcttcgcg agggccagcg agcccgcggt ggtcaccgaa
     5941 tccgcgagat cgatgttgtc cttgatgacg acgggaatgc cgtgcagcgg accgcgaatc
     6001 tttcccgcct tgcgttcgcg gtcgagcgcc gcggcatcac gcaacgcgtg cgcatgcgtc
     6061 gcgatgacgg cgtgcagcgt gtcgttgcag tcggcaatgc gcgcgagata tccctgaacc
     6121 agcagcaccg aggtcgtttt cttgttctcg agcgccgcac gaagatcggg gacggtcgcc
     6181 gtcgtgagat cgagcgagcc caccatcgcg tgttccgcgc aggcctgcag gcgcggcgca
     6241 aaggcgagca gcccggcggc gatcgatatc gaaagccggt tcaaaacgtg accgacgcga
     6301 acgccatggc gccgccatag ttccatcgca accggccgct gaagagattc ttctcgagat
     6361 cgaatcggaa acggaaatcg ttgtaaccca ggcccacgcc gaatctcggc gtcgcctgcc
     6421 agacgatggt cgctttcaga tccgaaaggc tgccgttgta ttcgttgtag tcgacgtaga
     6481 agtactgcgc ctgcgccgtg aagtagaaat tgtgcggcaa cgcccagatg ccgcgcagcc
     6541 ccagcaccgg cagtggcgca tccgtggaac cgctctcgga caactcggtt gaaaacgaac
     6601 caccggcgcc cgtgaccgtg gcatcgagcg ccagtcttgc atcgagcacg tgcacaccga
     6661 ttccggcggc gagctcatat ttatctgtgc gcttgaacgc gtagtcatac gagagctgca
     6721 tgacctcgag ttcccatcgc gccgtggtcg tcgcgccgac tggataggtt tcgtcgccaa
     6781 aattgatatc gcggtcgagc tcgttggtcg acgagcggtt gttcttgaaa taggtgccgc
     6841 ggatcgaatg tttgcccttg atgcgccaca gaccatctac ccggaaacgt tcgaagcgat
     6901 ccaagccaaa ggtatcgtcg aagtcgatgg tatcgccgac cctgccgcct tctccgtcga
     6961 gtcgaacctg gatgtccgtc gagagaaaga acccgcccat gtccacgacg gcggtggcgt
     7021 tgagtggatt gcggccttcc tgcgcgaccg tggcgccggc gattccgaaa aggaccgctg
     7081 tcattattat tgttttcata cgagacctcg ctgtggagcg agcgtagcgc acctggaata
     7141 tttcggggaa ccgttggccg ggttccggta ctcacgagtt gacaatacct gacgctggtg
     7201 atgctttcgg cctgcttacc cgtcgttggc attcgggagt acgatcccgc tgcccacgac
     7261 gggagcatca tgaaacgacg aagccttctg agcgccgcgg ccggcgcaat ggttctcgcc
     7321 ccgcagatcc atggttcccg cgcgattgcc gccgaacccg gtcgcgctaa agagttgtac
     7381 ggattgctcg gcaaactgcc accgcgcgat cgcaaggtcg gcgcgcaact cgtcgcaacc
     7441 gaagaccgcg ggagctacac gctcgagaca ctggttctcg atctcaacgg catcgagtcc
     7501 gccccggcgt acttcgcgaa acccaagggc aagacgggcc ggcttcccac ggtgctgttc
     7561 aatcactcgc acggtggtgg gtacaagatc ggcaagaccg aattcgtcga gggccgtgag
     7621 tacctgaaca atccgcccta cgcggagttc ctgacatcct tagggttcaa cgcgctgtgt
     7681 ttcgacgcgt ggattttcgg cgaacgcgcc aggaagaccg agctggagac gttcaaggac
     7741 atgctctggc atggccaggt gctctgggga atgatggtct acgactctct caaggccgtg
     7801 gactatctgg tcacgcgtcc ggacgtggat acgaagcggc tgggcacggt gggaatgtcg
     7861 atgggcagct cgacggcgca gtgggcgggc gcgctcgatg ctcgcctcaa ggtcgtcgtc
     7921 gacatctgct gcctcacgga ctggcacacg ctggtcgaag tagatgggct gaaagggcat
     7981 ggcatctact actacgtgcc ggacctgctc aatcacttca cgaccgcgca gatgaatgcg
     8041 ctgatcgcgc cgcgcgcgca tttgtcgctg gcgggaaacc aggacaagct cacaccgctg
     8101 gcggggctcg aaaaaatcga tgcggagctg aagaaggtct acgcctcggc aggtcatccg
     8161 cagaactgga agctgctgcg ctacgacggc gggcacctgg aaaacccgca gatgcgtgaa
     8221 gagatccgcc gatggttcgt ggagaagctc tgagcgccac gaactactgc aaggcaggct
     8281 gattcacttc gaccaccgcc cgattacgga tggcgacgct tccgcccagg aatgcggtgg
     8341 gcccgatctc cattgcgtag agcagaacac gactgccacc ctcttcgcgc atggacgtcg
     8401 gcggccccaa cgagctgatg acttccagct cgctcatgcc gggccgaatc ttcttccacg
     8461 tcgatgcatc tacccatcgc ggaaggttgg tctcgggtga ggcgccgcgc gccggccgcg
     8521 gcccggacaa ttgcggatcg atgcggtcgg gacgcagccg catctcatcc atttgcctcg
     8581 acaggacctg cacctgccgc tgcaggttgc gcacttcctg ttcgaggaac gagaagcggc
     8641 cttcatccgc ggccgaagcc gtgagcggca ggagcagtaa cgcgagaagc agccgggcca
     8701 tggaagtctc ctttgcgaag aaaaggcgaa acgggaatgc ccgggcctgg actattgcag
     8761 tcaatgaata gacgtagtgg cgcaggctgc gcccgtgaat tcgtatcgcc tggcggagag
     8821 ttgcggatag acagccagca gtttgagcag gacgccattg gtccagccga aaccgtcctg
     8881 caccgggtac tcgccgcccg agccttcgcc ggcatcccgc acgttgtact tctcgacgag
     8941 tttgcccgtc ttgcaataca cgcgcgcatt ctcggcgacc cagccttcgg cgatcttcgc
     9001 cgcaagatcg gggtcgccgt tcttgcgaag tccttcgatg gctatccact gcaacggcgc
     9061 ccatccattc ggcgcatccc actgctgtcc gctggtttcg gtcgtcgtcg cgaggccgtg
     9121 cggcgtcagc aacgactcac gcagggtgct ggcgacgcgc tgcgcctgct gactcgaagc
     9181 gagtcccaca tacaacggaa acaccgtcgc ggcggtcagc cgcgtcgaag tcaccttgtt
     9241 tcgccaatcg tagtcggtaa aggcatcgag ctcgcggctc cacatcaccc ggagcatcgc
     9301 cgtctgacgc gccagggcgt tcgcgcgcat tctcgtgtcg cagtccggac gcttcgcctc
     9361 ttcgcaccca cgcgcgatcg tgagctcgag tttgtacagc aggctgttga gatccacggg
     9421 caggaaatcc gtcgtatgga tcgtgctcag ggttttgccg tcggccagcc accgggaact
     9481 gaaatcccaa ccgctttcgg cagcggcgcg cagatcacga tagacgtcgg gcgcggcgcg
     9541 cgtgctgcct ttcgcggttt cgaggtcttc gagataggac tcgtcgcgcg gaatcgcgag
     9601 gtcatcccaa tagcgattca acaggcttcc atcatcgaga cgcaccacgc ggcggtgcgc
     9661 ggtgccgggg gtcagcgacg cggcgccttc catccagaat tcatactcgc gctcgagctg
     9721 cggcaaccgc ctgatcattg cgcgcgagtc gtgttcggcc tgaagttcga ccatcgccgc
     9781 gaagaacggc ggttgtgagc ggctcaggta gtaagtgcgg ctgccgttgg ggatgtgccc
     9841 gaaccggtcg atgagtcccg cgaagttctc gaccatgtcg tcgatgagat cttcgcgacc
     9901 gctcacctgc agtccgagca tggtgaagta ggaatcccag taatacagct cgcggaagcg
     9961 cccgccgggc accacgtaac gagtcgagag ccgaagcagc gagccgccat ccggcaatcc
    10021 tttgggcgcg cgcacgagcg cgttccacag ctggttgatg tgcacgcgaa tttcctggcg
    10081 cggagtcgtc tgatagtcgt tccccgcgac ggatggtacg tcgaaatgtg cggccacgaa
    10141 gcgatcgagg gaaaagccct cgccattacg gctcttctcg tagtcgcgca cgattttcgc
    10201 gggtgcggtt ctcgccacgg cgtcggcaaa cgtcttgctg tcggcaaaca acttcccggt
    10261 ctgtacgcga tgaaaaagct cgccatacaa ctgagaaggc ggacgcggag ccgcatcgcg
    10321 cgactgcgcg acgactcccg cccaaacgag gagcaatgcc gcgatcgcag ggagaaccct
    10381 ggcgcgaata ctcggtaccg aggagcacat tccggtgctt ttatccgagg caatcgcgcg
    10441 cggcaatcgg aatgaagggc gaggagcgag gccatgcaca catgtagtca gatgctgacg
    10501 tgcgcattcg ccgcatctcc gggactcgag ccggggcgcc a
//
LOCUS       DATDEU010000528         3253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1125316, whole genome shotgun sequence.
ACCESSION   DATDEU010000528 DATDEU010000000
VERSION     DATDEU010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125316"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VIV63_12610"
     CDS             <1..717
                     /locus_tag="VIV63_12610"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125486.1"
                     /translation="AVWVGNASGEGRPGLTGSTMAAPLLFSLFNSLPASPWFDMPLHA
                     LRRIDTCANDGFLATASCETVSTWVPRDSHFDAQSPHNLRVSLDGSGKQRVHSDCESP
                     GRMLQASWFVLPPAEEFYFRRVHAEYRPLPALRADCAATRAGGRPALALLYPDPNAHV
                     LIPRELDGQTGRAVFEAVHRRRDATVYWHLDDRYLGETHTFHQQSLDMDPGEHILTVV
                     DDEGERVSRRFQVIATRNGL"
     gene            707..1582
                     /locus_tag="VIV63_12615"
     CDS             707..1582
                     /locus_tag="VIV63_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6125487.1"
                     /translation="MACNEGVVFDGFTLTTVETDEVTIRLRHGGSGPPLLLLHGFPQT
                     HAMWSKIAPRLAENYTVVCPDLRGYGGSSKPESSANHETYSKRAMARDQLEVMKKLGF
                     DKFHVAGHDRGGRCAYRLALDSPASVSKLAVLDIVPTGDTLRRADWRFGMGYWHWFFL
                     AQAHPLPERMISADPIAFLFRHGQGSSDFEAYEDFLQNARDPAAIHAMCEDYRAAVSV
                     DFQQDQADRGTRKIECPTLVLWGNKGVIPKWYDAVGIWKEWANDVRGEEIESGHMLAE
                     EAPDATYQALRAFFG"
     gene            complement(1593..1856)
                     /locus_tag="VIV63_12620"
     CDS             complement(1593..1856)
                     /locus_tag="VIV63_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125488.1"
                     /translation="MAKQQKIQGEGDYEATRRYRKRTDEFLNNNDVEKAAVRAAPTSA
                     AEAEDLKAAEAAGKRRAKGEDPALRRRSAPRVEPSTRGKTSRN"
     gene            2016..2231
                     /locus_tag="VIV63_12625"
     CDS             2016..2231
                     /locus_tag="VIV63_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125489.1"
                     /translation="MASITTAWVGRTSLIDFAEAGAEPPNLARHKLDRLRELRAVIDS
                     LRCERESRDPLLRWIIDRERAESLKLD"
     gene            2297..2677
                     /locus_tag="VIV63_12630"
     CDS             2297..2677
                     /locus_tag="VIV63_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6125490.1"
                     /translation="MSAPPLRVLIAEDNDDLRGLLVTLVSAEPDLKCVGTAADVESVI
                     ASTTENIADVLVLDLELQGRSSIDLLRTVRRNGSKITIVVFSGHSHPEMIRHTLAAGA
                     DAYIPKSGDYELLLDAIRDRRATV"
     gene            complement(2714..>3253)
                     /locus_tag="VIV63_12635"
     CDS             complement(2714..>3253)
                     /locus_tag="VIV63_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEY6125491.1"
                     /translation="VATVPAAFADETAGWYLGGGIGQFNAQIDDLEEVDDTIEGWDND
                     DTAYKFFAGYRLNKVLAFELDYINLGEPSGAVVPGLNVDAAVDGFAPYVVATLPLGNF
                     FEVYARLGYFFYDATYGVENELDDRVEFDEESEDLVYGAGIGANIGEKLNIRFEYEKF
                     DLEGMDDADSLWLTAAWRF"
BASE COUNT          592 a         1087 c          982 g          592 t
ORIGIN      
        1 gccgtgtggg tgggcaacgc cagcggcgag gggcgccccg gactcacggg ttcgacgatg
       61 gccgcacctc tgctgttttc attgttcaac tcgctgccgg ccagcccctg gttcgacatg
      121 cccctgcatg cgctgcggcg catcgacacc tgcgcgaacg acggctttct cgcgaccgcc
      181 tcctgcgaaa cggtgagcac ctgggtcccg cgcgacagcc acttcgatgc gcaaagcccg
      241 cacaacctgc gcgtgagtct cgatggcagc ggcaaacaac gtgtgcacag cgactgcgaa
      301 tcgccggggc gcatgctgca ggccagctgg ttcgtgttgc cgccggccga agagttctac
      361 tttcgtcgag tacatgccga gtaccgtccg ctgccggcgc tgcgcgcgga ctgcgccgcc
      421 acgcgcgcgg gcggtcgtcc agcgctggcg ctgctatatc ccgatccgaa tgcacacgtg
      481 ctgattcccc gtgagctcga tggacagact ggccgggccg tattcgaggc cgttcaccgg
      541 cgtcgggacg cgacggttta ttggcacctc gatgaccgat accttgggga aacgcacact
      601 tttcaccagc agtcgttgga catggacccc ggcgagcaca tactgaccgt ggtcgatgat
      661 gagggggaac gcgtctcgcg tcgcttccag gtcatcgcca cgcggaatgg cttgtaacga
      721 gggtgtcgtg ttcgatggat tcacgctaac aactgtcgag accgacgaag tcaccatccg
      781 ccttcgccac ggcgggtcgg gcccccctct cctgctcctg cacggattcc cgcagaccca
      841 cgcgatgtgg tcgaagatcg cccctcgcct cgcggaaaac tacaccgtcg tgtgccccga
      901 tctgcgcggt tacggcggca gcagcaaacc cgaatcgagc gcgaatcacg aaacctattc
      961 gaagcgcgcc atggcgcgcg accagctcga ggtcatgaag aagctcggct tcgacaaatt
     1021 ccacgtcgcc ggccacgatc gcggcggacg ctgcgcctat cgactcgcgc tcgattcacc
     1081 cgcctcggtc agcaaactcg cggtgctcga catcgtcccc acgggcgaca cgctgcgccg
     1141 cgccgactgg cgtttcggca tgggctactg gcactggttc ttcctcgcgc aggctcatcc
     1201 gctgcccgag cgcatgatca gcgcggaccc catcgcgttc ctgttccgtc acggccaggg
     1261 gtcttcggac ttcgaggcct atgaagattt cctgcagaac gcgcgcgacc cggctgccat
     1321 ccacgccatg tgcgaggact atcgcgcagc ggtcagcgtc gacttccagc aggaccaggc
     1381 cgatcgcggc acgcgcaaga tcgagtgccc cacgctggtg ttgtggggca acaagggagt
     1441 gatccccaag tggtacgacg ccgtcggcat ctggaaagaa tgggccaacg acgtccgcgg
     1501 cgaagagatc gagagcggcc acatgctggc cgaagaagca cccgacgcca cttaccaggc
     1561 gctacgggcg ttcttcggct gacgaccgct aactagtttc gagaagtctt gccgcgcgtg
     1621 gacggctcga ctcgcggcgc cgatcgccgg cgcagcgccg gatcctcgcc cttagcgcgt
     1681 cgtttgcccg ccgcttctgc cgccttgaga tcctcggcct cggcggccga agtcggggct
     1741 gcccgcaccg ccgccttctc cacgtcgttg ttgttcagga attcgtcggt tcgcttgcga
     1801 taacggcgcg tggcttcgta gtcgccctcg ccctggattt tctgctgctt cgccatggca
     1861 attcctccgt ctgggaaatc cattagacct aattttcggg cgccattggc gcgctgcggc
     1921 accgtcctac gccacaatca gcgcacacgc agattcgagt cccacgcggg tttgcgagac
     1981 tggctccatc aaggaacgat gcggagtcgt cgaccatggc aagcattaca accgcgtggg
     2041 tggggcgaac ctccctcatc gacttcgccg aggcgggcgc cgaaccgccc aacctggcgc
     2101 gccacaaact cgatcgactg cgcgaactgc gggcggtaat cgactccctg cgctgcgagc
     2161 gggaatcgcg ggaccccctg ctgcgctgga tcatcgaccg ggaacgcgcc gaaagtctca
     2221 agctcgattg aataccgggt cgtccggctg cgacaatggc cgccggatga ggttttgcag
     2281 gtagtcttca cgcgtcatga gcgcgccacc ccttcgagtc ctgattgccg aagacaacga
     2341 cgaccttcgc ggtctcctgg tcacgctcgt ctcggctgag cccgatctca aatgcgttgg
     2401 cacggctgcc gacgtcgaat cggtcatcgc cagcaccacc gagaacatcg ccgacgtgct
     2461 ggtgctcgac ctcgagctcc agggacgatc gagcatcgac ctgctgcgca ccgtacgccg
     2521 caacggcagc aagatcacta tcgtggtttt ctccgggcat tcacatccgg aaatgatccg
     2581 tcacacgctc gcggccggcg cggacgccta tattccgaag agcggtgact acgaactact
     2641 gctcgacgcc atccgcgacc gtcgcgcgac ggtctgacgc cggaacgcac acgcgctccg
     2701 gcgccagccg gttctagaaa cgccaggccg cggtcagcca aagcgaatcg gcgtcatcca
     2761 tgccttcgag atcgaacttc tcgtactcga agcggatgtt gagcttctca ccgatgttgg
     2821 cgccaatacc ggcgccgtag acgaggtctt cggactcttc atcgaattcg acgcggtcgt
     2881 cgagttcgtt ctcaacgccg taagtggcat cgtagaagaa gtaaccgagc cgggcataca
     2941 cctcgaagaa attgcccagc ggcagagtgg caacaacgta tggagcgaat ccatccacgg
     3001 ccgcatcgac gttgagtccc ggcacgactg cgcccgaggg ttcgcctaag ttgatgtagt
     3061 cgagctcgaa agccagcacc ttgttgaggc gatagcctgc gaagaacttg tacgcggtgt
     3121 cgtcgttgtc ccagccttca atggtgtcgt cgacttcttc gaggtcgtcg atctgcgcat
     3181 tgaactgacc aatgccgcca ccgagatacc agccagcagt ctcgtcggca aaggcagcgg
     3241 gcaccgtcgc tac
//
LOCUS       DATDEU010000529         5070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_542086, whole genome shotgun sequence.
ACCESSION   DATDEU010000529 DATDEU010000000
VERSION     DATDEU010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542086"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VIV63_12640"
     CDS             complement(<1..359)
                     /locus_tag="VIV63_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125492.1"
                     /translation="MSEQSSSGGVSYKQVGSDYFEKRGLKRYAKVWSLWALGVGAVIS
                     GHYSGWNFGLANGFGSMLVALFIIAAMYWGLIFSLAEMSPALPHTGAAYSFARSAMGP
                     WGGMFTGLAESIEYILTP"
     gene            complement(591..1616)
                     /locus_tag="VIV63_12645"
     CDS             complement(591..1616)
                     /locus_tag="VIV63_12645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY6125493.1"
                     /translation="MRILIVTDAWEPQVNGVVRTLKTTARELGSMGHEVRFLTPLEFR
                     TLPCPTYPEIRLSLLPQSGVNARIREFDPDVLHIATEGPLGLAARRYALRNDFPFTTA
                     YHTRFPEYVHARLRLPLSWSYAWLRHFHGPSKGVMAPTQVVVDDLRANGFDNVRLWSR
                     GVDTDVFHPQPSKRLDSTPPIFLYVGRVAVEKNVEAFLELDLPGSKWVVGTGPALEGI
                     RLRHPRVNYLGLLDREELAKVYAAADVFVFPSKTDTFGLVLLEAMACGLPVAAYPVTG
                     PRDVIADSKAGVLDTDLRAACLGALALKREDAVARAGLFTWRAATEQFLGHLHPRKRA
                     STMPVFA"
     gene            complement(1689..2720)
                     /locus_tag="VIV63_12650"
     CDS             complement(1689..2720)
                     /locus_tag="VIV63_12650"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY6125494.1"
                     /translation="MRTIEPIQHLRALAALGVVIFHSLGLLGTHLGIVVTHKSLGAAG
                     VDLFFIISGFIMWVTAIDRDERPGTFALKRIVRIVPLYWLFTTFVLALVIIKPSLMRS
                     ASQDPVHFLASYLFVAWPHPTMVGRLWPPVIPGWTLNYEMFFYATVTLSLLLERAWRV
                     PAIAAVLVGLPVLGWVSQPEGIASFYTHPILLEFLFGIALGVLFTRGYTLTPRRAWGV
                     TLVALAMFFSLGLLGDDDNRTLMWGVPLAFLVAGALELPALAQGRVRDFSKLLGDASY
                     SLYLVQFVVLPPASAVMIRLVRPWPLPLAATVMVAGLIVVAVVAGIATYYIAERPIMQ
                     FARRWIKRS"
     gene            complement(2734..3567)
                     /locus_tag="VIV63_12655"
     CDS             complement(2734..3567)
                     /locus_tag="VIV63_12655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY6125495.1"
                     /translation="MPRILVIDGNEKATRDKHVSMGGTSSGEGYAATLQRLKPGIECD
                     IVRPADEEPTLPEGMSLENYHGAVITGSALNVYSREASVERQIDLAKAVFAAGVPCFG
                     SCWGLQVGVTAAGGSVVRNPRGREFGFGRRITLTNQGRDHAMFHGKPEVFEAATVHVD
                     TVETLPAGATALAHNDMGLQAAEIKLKKGSFWGVQYHPEYSCAEIAAMARRYGEVLIR
                     DGLVKDQAELDTLAADLVALHENPGDARLAWRFGVGPSITDPGIKLAELRNWLDRQVL
                     K"
     gene            complement(3573..4739)
                     /locus_tag="VIV63_12660"
     CDS             complement(3573..4739)
                     /locus_tag="VIV63_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY6125496.1"
                     /translation="MTIPMKGNWTYPTRVWSGPGRIAELPAACADLGIHKPLVVTDIG
                     LRDSQMIKRAVASLSGARLFADVRGNPVAANVEAGLEAYKAGDHDGVVAFGGGSGLDA
                     GKVIAFMSGQSRPLWDFEDVGDWWTRADPKGIAPIVAVPTTAGTGSEVGRAGVILNEE
                     THQKKIIFHPKMMPGIVIADPELTVGLPAKITAATGFDAWVHCFEAFCAPGFHPLADG
                     VALEGMRLIKIYLPRACKNGGDLEARSRMLAAASMGATAFQKGLGAVHAIAHPVGSFF
                     DTHHGLTNAVITPYVIVHNRDAIAERMKVVARVLDLPKPGIDGVLDWVLAFRKELNIP
                     HSLSEIGVTLRNPEEIGREAALDPSAGGNPIPVDAATLERIFRSAVKGDLSLKS"
     gene            complement(4736..>5070)
                     /locus_tag="VIV63_12665"
     CDS             complement(4736..>5070)
                     /locus_tag="VIV63_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6125497.1"
                     /translation="VDHRMTVMREEIFGPVAGIMKVSSDDEAVDLMNDSDFGLTAAVW
                     TEDADAALALGQRVNTGTWFMNRCDYLDPALAWVGVKDSGRGCTLSVVGYEHLTRPKS
                     FHMRLKTS"
BASE COUNT          959 a         1669 c         1595 g          847 t
ORIGIN      
        1 ggcgtgagga tgtactcgat gctctccgcg agcccggtga acattccgcc ccagggcccc
       61 atggcgctgc gtgcgaacga atacgcggcg ccggtgtgcg gcagcgcggg agacatctcg
      121 gcgagactga agatgagtcc ccagtacatc gcggcgatga tgaacagtgc gaccagcatc
      181 gaaccgaagc cgttcgcgag tccgaagttc cagcctgaat agtggcccga gatcacggcg
      241 ccgacgccga gagcccatag cgaccacact tttgcgtagc gcttcaggcc acgcttctcg
      301 aaatagtccg agccgacctg cttatagctc acgccgccgg acgatgattg ctcgctcatg
      361 cgttaactcc ccgaaaaagg ggacagatct atttttctgc ccctgcttat tgttctagtt
      421 gcgtgcctgg gaacagaaaa atagatctgt cccctttttc gggaccgagt ataggccgag
      481 gcctcggatg gcccgcaaga gaaacgccta aagggtggcg tgaagtattt cggggttact
      541 acggctcaat ttaggtgcac agggtgcaac tgattgtttg ccttcaggaa tcacgcaaac
      601 acaggcatcg tcgatgcgcg cttgcgcgga tgcaggtggc ccaggaattg ctcggtggcc
      661 gcgcgccacg tgaaaagtcc cgcgcgtgcg acggcatctt cgcgtttgag tgcgagcgcg
      721 cccaggcacg ccgcacgcaa atccgtgtcg agcactcccg ctttcgaatc ggcgatgaca
      781 tcgcgcggac cggtgaccgg atacgccgcc acgggcagtc cgcacgccat ggcttccagc
      841 aacacgaggc cgaaggtatc ggtcttgctc gggaacacga atacatcggc ggcggcgtac
      901 accttcgcca gctcttcgcg gtctaacaac cccaggtagt tgacgcgcgg gtgacgcagg
      961 cggatgccct cgagcgcggg acccgtgccg accacccact tggatccggg caggtcgagc
     1021 tccaggaagg cctccacgtt cttctcgacg gccacgcggc cgacgtacag aaaaatcggc
     1081 ggcgtgctgt cgagacgttt gctcggctgc ggatgaaaga cgtcggtgtc gacgccgcgc
     1141 gaccacaggc ggacattgtc gaagccgttg gcgcgaagat cgtcgaccac cacctgcgtc
     1201 ggcgccatca cacccttcga gggtccgtgg aaatgccgca accacgcgta gctccacgac
     1261 agcggcaggc gcagccgcgc gtgaacgtac tcggggaaac gcgtgtgata ggccgtggtg
     1321 aacggaaaat cgttgcgcag tgcgtatcgc cgcgccgcga gaccgagcgg cccttccgtc
     1381 gcgatgtgca gcacgtccgg atcgaactcg cggatacgcg cattgacgcc gctctgcggc
     1441 agcagcgaca ggcgaatctc gggataggtc gggcacggca acgtgcggaa ctcgagcggt
     1501 gtcaggaagc ggacttcgtg acccatggat ccgagctccc gcgccgtggt cttgagggtg
     1561 cgcacgacgc cgttgacttg cggctcccag gcgtccgtga caatgaggat tctcaaatat
     1621 gcctcctaac cagttgaggc atagtccatc gcgaattcgt tacatcgcga tgacagtgcg
     1681 cggcgtcttc aggagcgttt gatccagcgc cgtgcgaact gcatgatcgg ccgctcggcg
     1741 atgtaatacg tcgcgatccc ggcgacgacc gccacgacga tgaggccggc caccatcacg
     1801 gtcgccgcca gtggcagcgg ccacggccgc accaggcgaa tcatcacggc cgacgccggc
     1861 ggcagcacga cgaactgaac caggtagagc gaataggaag cgtcaccgag cagcttcgag
     1921 aaatcacgca cccttccctg cgccagcgcg ggcaattcca gcgcgccggc caccagaaac
     1981 gccagcggca cgccccacat gagcgtgcga ttgtcgtcgt cgccgagcag ccccagcgaa
     2041 aagaacatcg cgagcgccac gagcgtgact ccccacgcgc ggcgcggcgt cagcgtgtaa
     2101 ccccgcgtga acaacacgcc gagcgcgatg ccaaacagga attcgagcag gatcggatgc
     2161 gtatagaagg aggcgatccc ttcgggctgc gacacccacc ccaggaccgg caggcccacc
     2221 agtacggccg cgattgccgg cacgcgccac gcgcgctcga gcagcagcga cagcgtgacg
     2281 gtcgcataga agaacatttc gtagttcagc gtccagccgg gaatcaccgg cggccagaga
     2341 cgccccacca tcgtgggatg cggccacgcc acgaacagat aggacgcgag aaaatgcacg
     2401 ggatcctggc tcgcactgcg catgagggac ggcttgatga tgaccagcgc cagcacgaag
     2461 gtcgtgaaca gccagtacag cggcacgatg cgcacgatgc gcttcagcgc gaaagtgccc
     2521 ggccgctcgt cacggtcgat ggccgtgacc cacatgatga agccgctgat gatgaagaac
     2581 aggtcgacgc ccgccgcccc caggctcttg tgcgtgacga cgatacccag atgcgtgccc
     2641 aacaatccca gcgaatggaa aatcaccacg ccgagcgccg ccagggcgcg caggtgttgt
     2701 atgggttcga tcgtacgcac gcggtcctgc gtgttacttc agcacttgcc ggtcgagcca
     2761 gtttcgaagt tcggcgagct tgatgcccgg atcggtgatc gacggcccta cgccgaagcg
     2821 ccacgcgagc cgggcgtctc cgggattctc gtgcaaggcc acgagatccg ccgcgagcgt
     2881 gtcgagttcc gcctgatcct tgacgagccc gtcgcgtatg agcacctctc cataacgccg
     2941 cgccatcgcc gcgatctccg cgcagctgta ttccgggtgg tattgcacac cccagaagct
     3001 gcccttcttc agtttgatct cggccgcctg caatcccatg tcgttgtgcg ccagcgccgt
     3061 ggcgcctgcg ggcagtgtct cgacggtatc gacgtgcacg gtggccgctt cgaacacttc
     3121 gggtttgccg tgaaacatcg cgtgatcgcg accctgattc gtgagcgtga tgcgacgtcc
     3181 gaagccgaac tcgcggccgc gcggattgcg cacgacgctg ccgcccgccg cggtgactcc
     3241 cacctgcaga ccccagcaac tgccgaaaca cggcacgccc gccgcgaaca cggccttggc
     3301 gagatcgatc tgccgctcca ccgaggcttc ccgtgaatag acgttgagcg cggagccggt
     3361 gatcacggcc ccgtgatagt tctcgagcga catgccctcg ggcagcgtcg gctcttcatc
     3421 ggcgggccgc acgatgtcgc actcgatgcc gggcttcagc cgctggagcg tggctgcgta
     3481 gccctcgccc gacgacgtgc cacccatcga cacgtgtttg tcgcgcgtcg ccttctcgtt
     3541 gccgtcgatg accaggatgc ggggcactac tttcagcttt tcagggagag gtcgcccttc
     3601 accgccgaac ggaagattcg ctcgagcgtg gccgcgtcca ccggtatggg gttgccgccc
     3661 gccgaaggat cgagggccgc ctcgcggccg atctcttccg ggttgcgcag cgtcacaccg
     3721 atctcggaca gcgaatgcgg aatgttcagc tccttgcgga aagccagcac ccagtcgagc
     3781 acgccatcga tgcccggctt cggcaggtcg agcacgcgcg ccacgacttt catgcgctcg
     3841 gcgatggcgt cgcggttgtg cacgatgaca tagggcgtga tgaccgcatt cgtgaggcca
     3901 tgatgcgtgt cgaagaacga acccacgggg tgcgcgatcg catgcacggc gccgagcccc
     3961 ttctggaaag ctgtggcgcc catgctggcc gcggccagca tgcgggaacg cgcctcgaga
     4021 tcaccgccgt tcttgcaggc ccgcggtaga tagatcttga tgagccgcat gccttcgagc
     4081 gccacgccat cggccagcgg gtggaaaccc ggcgcgcaga acgcttcgaa gcaatgtacc
     4141 cacgcgtcga aacccgtggc cgccgtgatc ttggccggca gacccacggt gagctcggga
     4201 tcggcgatga cgatgcccgg catcatcttc ggatggaaaa tgatcttctt ctggtgcgtc
     4261 tcttcgttga ggatgacgcc cgcgcggccc acctcactac ccgtgccggc cgtggtgggt
     4321 accgcaacga tgggcgcaat gccttttgga tcggcgcgcg tccaccagtc gcccacgtcc
     4381 tcgaaatccc acagtggccg cgactgtccc gacatgaagg caatgacctt gccggcatcg
     4441 agccccgagc cgccgccgaa cgcgacgacg ccatcgtggt cgccggcctt gtacgcctcg
     4501 agccccgctt cgacgttggc cgccaccggg ttgccgcgca cgtcggcaaa caatctcgcg
     4561 ccggacagcg aggccaccgc gcgtttgatc atctgcgaat cgcgcaggcc gatgtcggtg
     4621 acgacgagag gtttgtggat gccgagatcc gcgcacgcgg ccggtagttc ggcgatgcgt
     4681 cccgggcccg accagacgcg tgtcgggtaa gtccagttgc ccttcatcgg aatcgtcatg
     4741 atgtcttgag tctcatgtga aatgactttg gccgggtgag atgctcgtag ccgaccaccg
     4801 acagcgtaca gccacgcccc gaatccttca cgcccaccca ggcgagcgcg gggtcgaggt
     4861 agtcgcagcg gttcatgaac caggtccccg tgttcacgcg ctgacccagc gccagcgccg
     4921 cgtcggcgtc ctcggtccag acggcggcgg taagcccgaa atcactgtcg ttcatgaggt
     4981 ccacggcctc gtcatccgag ctcaccttca tgatcccggc aacgggcccg aagatttcct
     5041 cgcgcatcac cgtcatgcgg tgatccacgt
//
LOCUS       DATDEU010000530         3531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_832999, whole genome shotgun sequence.
ACCESSION   DATDEU010000530 DATDEU010000000
VERSION     DATDEU010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832999"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VIV63_12670"
     CDS             complement(<1..414)
                     /locus_tag="VIV63_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY6125498.1"
                     /translation="MKQLILVCLLAASSAAFAQAPAAGAAKVEADEAKTRAKLEEARA
                     RLDKAAREVAELSTQLGANARHDVRFIGGGPSKAVLGIQVDPRSDEKGVRVLGVSPGG
                     PAAEAGLRDSDVIIALDGVATTGSDPDRALITQMRK"
     gene            complement(435..914)
                     /locus_tag="VIV63_12675"
     CDS             complement(435..914)
                     /locus_tag="VIV63_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125499.1"
                     /translation="MDTEVKDAELISRLRRLDAAAVQSDVSFGYEGLIGRHAARQRRE
                     RRRHAFARGTASALVIAMVGVGVWRFEQQPVGSPDGVEAPSPVADAQASQPRIVRADT
                     YFAVAALEDHIASIDDALNDARLSGGAADVARLESTRALLVDSYTSVRYAEMVNANF"
     gene            complement(904..1500)
                     /locus_tag="VIV63_12680"
     CDS             complement(904..1500)
                     /locus_tag="VIV63_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6125500.1"
                     /translation="MQRLAEIHVPDDELRAARAGDAAARSALFQRVAPATFGIIRRLV
                     AQKAMAEDLLQDTLIAMFEHLDDFRGEAPFGVWVRSIAVSRCLMAFRSPWHRARVALE
                     SWTEESWTGPADAESRTSDLIDLDRALAKLSPVTRAVVWFYDVEGWSHDEIAKAFDRT
                     VSFSKSQLARGHARLRDDLAPPVPSGIGIPLRSEFDGH"
     gene            1579..1666
                     /locus_tag="VIV63_12685"
     tRNA            1579..1666
                     /locus_tag="VIV63_12685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1613..1615,aa:Leu,seq:caa)
     gene            1677..2213
                     /locus_tag="VIV63_12690"
     CDS             1677..2213
                     /locus_tag="VIV63_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6125501.1"
                     /translation="MNFCSNCGSRVVLKVPEDDFLPRHVCDNCGTIHYQNPKLVVGSV
                     PVYEGRILLCKRGIEPRLGYWAIPGGFMENDETLEAGAAREAQEEALIDVEIGSLLLV
                     ANVTHARQVHVFFRSRMRTPAFGVTHESLEVKLVDEKDIPWADLAFPSTEYALRRFVE
                     DRAAGVERHHLAEIRRRF"
     gene            2279..2602
                     /gene="fdxA"
                     /locus_tag="VIV63_12695"
     CDS             2279..2602
                     /gene="fdxA"
                     /locus_tag="VIV63_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY6125502.1"
                     /translation="MAFVVTEACIKCKYTDCVEVCPVDCFHEGPNFLVIDPEECIDCT
                     LCEPECPVEAIFSEDEVPAGQEEFKKLNADLSKKWPVIAEKKPAPPDAKQWEGVAEKK
                     KLLET"
     gene            complement(2622..3389)
                     /locus_tag="VIV63_12700"
     CDS             complement(2622..3389)
                     /locus_tag="VIV63_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY6125503.1"
                     /translation="MSFNSVRLLTVIAATLVAGSAFAPAFGQGAAVTPAAAPAKTDPR
                     AVIVNKLDGVKLEDVRMSPVNGMYEVTRGSEISYLSGDGRYAILGDLIDLDSDANISE
                     NRRRGIRNRMLESIPESEMLVYSPKDPKYTITVFTDIDCGYCRRLHSQMAEYNRLGIR
                     VRYLFFPRSGPDTESWHKAESVWCARNRNEALTKAKNGEAVNSPKCPANIIQRDYDLG
                     HKLLVEGTPAIFLPSGEMLPGYAPPGQLAKYLKTGKM"
BASE COUNT          624 a         1138 c         1138 g          631 t
ORIGIN      
        1 cttgcgcatc tgcgttatca acgcgcgatc ggggtcgctg ccggtcgtgg ccacgccatc
       61 gagcgcgatg atgacgtcgc tgtcgcgcag tcccgcttcg gcggccggtc cgcccgggct
      121 cacaccgagt acgcgcacgc ctttctcgtc gctgcgcgga tccacctgga ttcccagcac
      181 cgccttcgac ggcccgccgc cgatgaaacg cacgtcgtga cgggcgttcg cgccgagctg
      241 cgtcgacaac tcggcgactt cgcgcgccgc cttgtcgagc cgtgcgcgcg cctcttcgag
      301 cttggcgcga gtcttggctt catccgcttc gaccttggcc gcgcctgccg cgggcgcctg
      361 ggcgaacgcc gcggagctcg cggccaacaa acaaaccagt atcaactgtt tcatgaagat
      421 tctccgacag cccatcagaa gttcgcgttc accatttcgg cgtaacgcac actcgtatac
      481 gaatcgacca ataaggcacg cgtgctctcg aggcgagcga catccgccgc tccgccgctc
      541 aaacgcgcgt cgttgagcgc gtcgtcgatg ctggcgatgt gatcctcgag cgccgcgacc
      601 gcgaaatagg tgtcggcacg cacgatgcgc ggctggctgg cctgggcgtc ggccacgggc
      661 gatggcgcct cgacaccgtc cggcgagccc accggctgct gctcgaagcg ccacactccg
      721 acgcccacca tggcgatgac cagcgcgctg gccgtgccgc gtgcgaacgc gtgccggcgg
      781 cgttcgcgcc gctgcctggc tgcgtgccgg ccgataagcc cttcgtagcc gaagctcacg
      841 tccgactgaa ccgcggcggc atcgagccgg cgcaggcgcg agatcagctc cgcatccttc
      901 acttcagtgt ccatcgaact cactcctgag cggaatgccg atcccggacg ggaccggcgg
      961 cgccagatcg tcgcgcaggc gtgcgtgtcc gcgcgccagt tgcgacttgg aaaaactcac
     1021 cgtgcggtcg aacgccttcg cgatttcgtc gtgcgaccag ccttcgacgt cgtaaaacca
     1081 gaccaccgcg cgcgtgaccg gcgagagttt cgccagcgcg cgatcgaggt cgatgagatc
     1141 ggaggtgcgg ctttccgcgt cggccggacc ggtccagctc tcttctgtcc aggactcgag
     1201 cgcaacgcgc gcccggtgcc agggcgaacg gaacgccatc aggcaacggc tcacggcaat
     1261 actgcgcacc cagacgccga agggcgcttc gccgcggaag tcgtcgaggt gctcgaacat
     1321 ggcgatcagc gtgtcctgca ggaggtcctc ggccatggcc ttttgcgcca cgagccgccg
     1381 gatgatgccg aacgtcgccg gcgccacgcg ctggaacagc gcgctgcgcg ccgcggcatc
     1441 gcccgcccgc gcggcgcgaa gttcgtcatc aggaacgtga atttccgcga gtctttgcat
     1501 tttcttatgg cttagatgcc gccccgggga gaaaggtcgc aaatccgcgg atggtgttgg
     1561 ctatacttcc ggccccgcgc cgggatggcg gaactggtag acgcggcgga ctcaaaatcc
     1621 gttgccctta taaggcgtgt gggttcgatt ccctctcccg gcaccatttt cgactgatga
     1681 acttttgcag taactgtggc tcccgagtcg tcctcaaagt tcctgaggac gatttcctgc
     1741 cgcgccacgt ctgcgacaac tgcgggacca tccactacca gaatcccaaa ctcgtggtcg
     1801 gcagcgtgcc ggtgtacgag gggcgcatcc tgctctgcaa acgcggcatc gagccgcggc
     1861 tgggctactg ggcgattccc ggtggattta tggaaaacga cgagactctc gaggccggag
     1921 ccgctcgcga ggcgcaggaa gaggcgctca tcgacgtgga aatcggcagc ctgctgctgg
     1981 tcgccaacgt cacccatgcc cgccaggtgc acgtcttctt ccggtcccgg atgcgcactc
     2041 ccgcgttcgg cgtcacccac gagagcctgg aagtgaagct cgtggatgaa aaggacattc
     2101 cctgggcaga cctcgcgttt ccgagcaccg aatacgccct gcgccggttc gtcgaagacc
     2161 gcgccgcggg tgtcgaacgg caccacctgg ccgagattcg acgccggttc taggccagcc
     2221 tcatctagtg cagaatggcg cccccgaaaa acggggcagt tttcaaaagg atcgttcgat
     2281 ggcgttcgtg gtcacggaag cgtgcatcaa gtgcaagtac accgactgtg tggaggtgtg
     2341 cccggtcgat tgtttccacg aagggcctaa cttcctggtc atcgatcccg aagagtgcat
     2401 cgactgcacg ttgtgcgagc cggaatgccc ggtcgaagcc atcttctccg aagacgaagt
     2461 gccggccggc caggaagagt tcaagaaact caacgccgat ctgtcgaaga aatggccggt
     2521 gatcgccgag aagaagcccg cgccgcccga tgcgaagcaa tgggaaggcg tggcggaaaa
     2581 gaagaagctg ctcgaaacct gaaaaggttt cgcgctcgct cttacatctt gccggtcttc
     2641 agatatttcg cgagctgacc gggaggcgca taacccggga gcatttcacc gctcggcagg
     2701 aagatggccg gcgttccctc gaccagcaac ttgtgcccga gatcgtagtc gcgctggatg
     2761 atgttcgcgg ggcacttcgg tgaattcacc gcctcgccgt tcttcgcctt ggtgagcgcc
     2821 tcgttgcgat ttctggcgca ccacaccgat tccgccttgt gccacgactc ggtgtcggga
     2881 cccgagcgcg ggaagaacag gtaacgcacg cgaatgccga ggcggttgta ttcggccatc
     2941 tgtgaatgca accggcggca gtagccgcag tcgatgtccg tgaacaccgt gatggtgtac
     3001 ttcggatctt tcggcgaata caccagcatc tcgctctccg gaatgctctc gagcatgcgg
     3061 ttgcggatgc cgcggcggcg gttctcggaa atgttcgcgt cggaatcgag atcgatgaga
     3121 tcgccgagga tggcgtaacg gccatcgccc gacaggtagc tgatttccga gccgcgcgtg
     3181 acttcgtaca tgccgttgac gggcgacatg cgcacgtcct cgagtttcac gccgtcgagc
     3241 ttgttgacga tgacggcgcg cggatctgtt ttggcgggtg cggcggccgg ggtgacggcg
     3301 gccccttgtc cgaaggcggg cgcgaaggcg gatccggcca ccagggtggc ggcgatgacg
     3361 gtgagcaggc ggacggaatt gaagctcatg aggcgctctg gggtctgcgg cggtatgtcg
     3421 gattcgaccg cgcgcagacc atcaagttcg cgcgccagtc gacgcggaat cttactccaa
     3481 gcccgaggga acgcggcgcc ggaatgcgcc gccccggcat caacctcgcg g
//
LOCUS       DATDEU010000531         2864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_833042, whole genome shotgun sequence.
ACCESSION   DATDEU010000531 DATDEU010000000
VERSION     DATDEU010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833042"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VIV63_12705"
     CDS             <1..611
                     /locus_tag="VIV63_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY6125504.1"
                     /translation="VFNWTATMWKGSISFEPPMLFALAFLFLFTIGGFSGLMLAIVPA
                     DVQYHDTYFVVAHFHYVLVPGAIFAIMAGVYYWLPKWSGKMYDHKLANWHFWLSTIFV
                     NVLFFPQHFLGLAGMPRRIPDYSVQFADWNMVSSIGAFGFGLSQLLFPYIIWKCVKSG
                     SKAEGRSWEGAHGLEWELSSPPPYHSWDPPPSREIVARGVQH"
     gene            616..744
                     /locus_tag="VIV63_12710"
     CDS             616..744
                     /locus_tag="VIV63_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125505.1"
                     /translation="MTAMTPEEISERRAKARRTAILLGMVALSFYVAFIVMSVARS"
     gene            744..1277
                     /locus_tag="VIV63_12715"
     CDS             744..1277
                     /locus_tag="VIV63_12715"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY6125506.1"
                     /translation="MNENRKLTLKLGAFALGSFAFGFALVPLYDVICDITGFGNQKSL
                     AEQRLSVENPDDTRTITVDFMADLPSVGNWEFRPVVGSMQIHPGRLYKAEFIAHNLTG
                     HATTAQAVPNIMPGQAAGYFRKTECFCFVPQHFAVNEARPMTVRFVVDPAIPRSVDRI
                     TLSYTFYDETTRVGSLN"
     gene            1292..2179
                     /locus_tag="VIV63_12720"
     CDS             1292..2179
                     /locus_tag="VIV63_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY6125507.1"
                     /translation="MSNAHAPAGEKYYVPHSSKWPIIGSAAFFTLLLGVIALLNHWLP
                     AWALVPGFALVVYMFIGWFGDVISENNQGIYGLDVDRSFRMGMMWFIFSEVMFFAAFF
                     GALFYARSLSVPWLGGEGTKVLGSELLHPLFENAWPTNGPDRLGPRTDGSFEIIPAFG
                     LPAINTIILLLSGVTVTIAHHALRAGNRMVLNVFLAATFLLGFLFVYLQATEYAEAYN
                     HLGLTMSTGIYGSTFFMLTGFHGFHVTLGAIMLLCIWLRTMSGHFTPERHFAFEAVAW
                     YWHFVDVVWLGLFIFVYWI"
     gene            2358..>2864
                     /locus_tag="VIV63_12725"
     CDS             2358..>2864
                     /locus_tag="VIV63_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125508.1"
                     /translation="MPRLTCLILVVLAGCAKQQPAADATHELIVRGGTILDGSGSEGY
                     VGDVVVDGDRIVYVGKSRGDTAKTVIDATGKAVAPGFINMLSWAVASLLVDGRSQSDL
                     RQGVTLEVFGEGDSWGPLSPEMKKRAKERQTDIQYDIAWTSLGEYLEHLQSRGITPNV
                     ASYIGAATT"
BASE COUNT          472 a          937 c          852 g          603 t
ORIGIN      
        1 aggtcttcaa ctggactgcc accatgtgga agggctccat cagcttcgaa ccgcccatgc
       61 tgttcgcgct ggcgttcctg ttcctgttca ccatcggcgg cttctcgggc ctcatgctcg
      121 cgatcgtccc ggccgacgtc cagtaccacg acacctactt cgtcgtggcg cacttccact
      181 acgtgctcgt gccgggcgcc atcttcgcca tcatggcggg cgtgtactac tggctgccga
      241 agtggagcgg caagatgtac gaccacaagc tcgccaactg gcacttctgg ctctcgacca
      301 tcttcgtgaa cgtgttgttc ttcccgcagc acttcctggg actcgccggc atgccgcgcc
      361 gtatccctga ctactcggtg cagttcgctg actggaacat ggtgtcgtcg atcggcgcgt
      421 tcggcttcgg cctctcgcag ctgctgttcc cgtacatcat ctggaagtgc gtcaagagcg
      481 gctcgaaggc cgaaggacgt tcctgggaag gtgcacacgg cctcgagtgg gaactgtctt
      541 ccccgccgcc gtaccacagc tgggatccgc cgccgtcacg tgaaatcgtc gcgcgcggcg
      601 tgcagcactg aagtcatgac ggccatgacg cctgaagaaa tcagcgagcg gcgcgcgaag
      661 gcgcgccgca ccgccatcct gctggggatg gtcgcgctgt cgttctacgt ggcgttcatc
      721 gtcatgtcgg tggcgcgttc gtaatgaacg agaacaggaa gctcacgctg aagctcggcg
      781 cattcgcgct cggcagcttt gcgttcggct tcgcgctcgt tccgctgtac gacgtcatct
      841 gcgacatcac cggtttcggc aaccagaagt cgctggccga gcaacgcctg tcggtcgaga
      901 acccggacga cacgcgcacc attaccgtcg atttcatggc ggacctgccc tcggtgggca
      961 actgggaatt ccgcccggtg gtcggctcga tgcagattca tcccggccgt ctctacaagg
     1021 ccgagttcat cgcgcacaac ctcaccggtc atgcgaccac cgcgcaggcg gtgccgaaca
     1081 tcatgcccgg ccaggccgcg ggatatttcc gcaagaccga atgcttctgc ttcgtgccgc
     1141 agcatttcgc agtgaacgaa gcgcggccca tgaccgtgcg cttcgtggtc gatcctgcga
     1201 tcccgcgctc ggtggaccgc atcacgctct cctacacttt ctatgacgag accacgcgag
     1261 tgggctcttt gaactagcaa cagggttact catgtcaaat gcgcacgcgc ccgctggcga
     1321 aaagtattac gtcccccact cgagcaagtg gccgatcatc gggtcggcag cgttcttcac
     1381 gctgctgctc ggtgtcatcg cgctgctgaa ccactggctt cccgcctggg cgctggtgcc
     1441 gggcttcgcg ctcgtggtct acatgttcat cggctggttc ggcgacgtca tcagcgagaa
     1501 caaccagggc atctatggcc tggacgtcga ccgttcattc cgcatgggaa tgatgtggtt
     1561 catcttctcc gaggtgatgt tcttcgcggc cttcttcggc gcgttgttct acgcgcggtc
     1621 gctgtcggtt ccgtggctcg gcggcgaggg caccaaggtg ctcggcagcg agctgctgca
     1681 tccgctgttc gaaaacgcct ggcccaccaa cggccccgac cgtctcggcc cgcgcaccga
     1741 cggatcgttc gagatcatcc cggctttcgg tctgccggcg atcaacacca tcatcctgct
     1801 gctgtccggc gtcaccgtga ccatcgcgca ccacgcgctg cgggccggca accgcatggt
     1861 gctcaacgtg ttcctggctg ccaccttcct gctcggcttc ctgttcgtct atctacaggc
     1921 gaccgagtac gcggaggcct acaaccacct cggcctcacg atgtccacgg gcatctacgg
     1981 ttccacattc ttcatgctca ccggcttcca cggtttccat gtgacgctcg gcgccatcat
     2041 gctgttgtgc atctggctgc ggaccatgag cgggcatttc acgccggagc gccatttcgc
     2101 gttcgaagcc gtggcctggt actggcactt cgtcgacgtg gtctggctgg gtctgttcat
     2161 cttcgtctac tggatctgat ggaaaagggg acagatctat ttttcgtccc cctctgtcag
     2221 gcatccaaag ggctcagatc gaaaaataga tctgtcccct ttttcgcgag aagggcgcct
     2281 ccgggcgccc ttttcttttg gtgagtttcg cgcgtgtata ttcaacgcca ctctccggaa
     2341 gcgtcgagat ccctgccatg ccgcgactga cttgcctgat cctggttgtg ctggccggat
     2401 gcgcgaagca gcaacccgcc gccgatgcca ctcatgagct catcgtgcgc ggcggcacga
     2461 ttctcgatgg ctccggtagc gagggttacg tcggcgacgt cgtcgtcgac ggcgatcgca
     2521 tcgtctacgt cggcaagtcg cgcggggaca ccgcaaagac cgtcatcgac gccactggca
     2581 aggccgtcgc gcccggcttc atcaacatgt tgagctgggc cgtcgcgtcg ttgctcgtcg
     2641 acggccgatc gcaaagcgat ctgcggcagg gagtcacgct cgaggttttc ggcgaaggcg
     2701 actcgtgggg gccgctgtcc ccggagatga agaagcgcgc gaaggaacgc cagaccgaca
     2761 tccaatacga catcgcgtgg acttcgctgg gcgagtacct ggagcatctg caatcgcgcg
     2821 gcatcacgcc gaacgtcgcg tcgtacatcg gtgccgccac gacg
//
LOCUS       DATDEU010000532         8519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_686558, whole genome shotgun sequence.
ACCESSION   DATDEU010000532 DATDEU010000000
VERSION     DATDEU010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686558"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VIV63_12730"
     CDS             complement(<1..1279)
                     /locus_tag="VIV63_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY6125509.1"
                     /translation="MRSRYGALSIYSFLLACVAVGALLLALRGAEFPAWWAVLLGIGT
                     CLFVWQFGLRAPRLGLISMERLPQIGMLLVFDPAVAASICGIASLTWPLVSRRYSQGS
                     LKVAALRGIHNASMTALMLLLAGYAYLACDGRHPLKGLLLTDVLPLAAMALTAQAVNI
                     ALMMLFFLFDGREVRRIVTPGYALSDLIFVPAGVLGAVLFNTDRPAVFWLFAGLMLLF
                     VLSFNSIGSHRDPVQAERGPLTRLFEAGLKLQGARRVDDLAERILAETRSLFRFDELY
                     LVLVDRERRLMDIRLHERQQVRMPARMKPVDTGLFGWLISRAEPLLVPDWSRAPTELV
                     QRAESTEKPTGSLLAVPLVNDGIVLGLLSVQHTQTGVYAPADLNVIRQLGEQVAGALA
                     DARAFEDLESYRRHLEERVTERTEELEKANAEKE"
     gene            complement(1483..2415)
                     /locus_tag="VIV63_12735"
     CDS             complement(1483..2415)
                     /locus_tag="VIV63_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY6125510.1"
                     /translation="MRIPLLFLLLFTVANVASAARPAPVIFDTDIGTDIDDAYALIAL
                     IRRPDLELLGVTTVSSDAVARARLAAKLLQVAGPKWAGVPVYAGISTATQYMKQVEWA
                     DGFSSPALHADGGVEFMRQQINARPGEITLIAVGELTNIAALLKSEPGIGKKIRAISL
                     MGGAIYRGHAPGSKPEPEWNIKSNAQAARIVFTSGVPLLIAPLDSTADLKLTPELRVQ
                     IFARGTKVNDTLASLDQIWRYTNHWKGNDPTLFDVLAVELVNPRTPYKLASLNIEVAD
                     DGLTRPVDGARPNSEVALEVNAAVFLQDFVARLQ"
     gene            complement(2440..3429)
                     /locus_tag="VIV63_12740"
     CDS             complement(2440..3429)
                     /locus_tag="VIV63_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6125511.1"
                     /translation="MQTFNRRHMLALAGHLLAVNTVASCAIRESAGEGSPFDAAGFTK
                     ARQFTRTPLGEIAHVSRGSGRAVLLLHGFPLNGFQWRGVIERLAPHALCIAPDFLGTG
                     HTRSSRNQDVGPDSQVLMLASLLDALGVAQADLIANDSGGAVAQLFTARYPTRVRSLL
                     LTNCDTEIQSPPAAMLPVIDLARQGRFVDEWLMPWHADPGLARSKDGIGGLCYANPAH
                     PTDEALQMYFEPLVNSAERKSALHRYAVALGHNALLGIQPALQRCRVPTRIVWGIDDT
                     IFDVSNADYLHRSFGNSRGVRMLSGAKLFWPEERPDIIAAQARILWESSAAQG"
     gene            3539..4759
                     /locus_tag="VIV63_12745"
     CDS             3539..4759
                     /locus_tag="VIV63_12745"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEY6125512.1"
                     /translation="MKTNPRLLARYLGAPALALALANAPASDHLDTAAVIADPASDIG
                     DLYAWASDGKRLNLVMTIVGGKFSDHVRYEFHIDSGTSPDHSAATISIACEFNSAFGP
                     DCRLGDVDRARGDASGEAGLASEKRRFRVFAGLRDDPFFNNVRGTRAALNAAGAALAN
                     TRRSDAGCPAFDQATTSRLLEEWRHTDGKPGQNFLEGWTTAALVIEVDLEAVRIGGSL
                     LGVWATTTVRGVTPGHPRVLDDGVPIDRMGRALTANALLGTFDTQEVSNRRKSKYNQA
                     PRAEWASFAPDIATTLAIYDGFDGVCGNQWMAGKQGKPAARFERLASMLADDRLWVNS
                     AAPHCASYLAVELELSGDCGGRTPNYDAVDVFRSLLVGGRVQGVDDGVVRDDRRHSES
                     GFPFLAAPVAKGAD"
     gene            complement(4712..5470)
                     /locus_tag="VIV63_12750"
     CDS             complement(4712..5470)
                     /locus_tag="VIV63_12750"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY6125513.1"
                     /translation="MASLLELQGAFAAALRDPSAPCPVLPVGNLAIYRNNSALSFRAA
                     LESSYPVLRRRVGDDYFRQLCSLYRARFPSRSGDLHWSGADFPAFLLEHLHGGDYAWL
                     TDLARLEWAREHASIARIESALTADALAAFAPAQLEHLVFTLQPSLSLLASDYPVFTV
                     WETNQVVNAPPVNQSLGPEVGMVRLRIDGIDIRQLPAPLFSYLSALSAGAPLGDAIGS
                     AGLDQEGVVSALGFVFNEGLVSALRDGRGEKRES"
     gene            complement(5454..6311)
                     /locus_tag="VIV63_12755"
     CDS             complement(5454..6311)
                     /locus_tag="VIV63_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY6125514.1"
                     /translation="MPRLESRGVREPIPARAGIGLRAIHHDALVTDRPAVGWIEAHTE
                     NYFHDGGPAVRALERARAHYPLSLHGVGLGLGSAEGVDREHLSRVKRAITRFEPALVS
                     EHACWGHAGGEFFNDLLPLPYTEEAALLLARQVGEAQDFLGTRLLIENVSAYVSFEHS
                     TLHEWEFLAAVAATSGCGLLLDVNNIYVSSRNLGIDANAFINGIPVDRVGEIHLAGHA
                     RNGDVFIDDHGSKVCEDVWRLYRSAIYRFGAQPTLIEWDTDIPDLATLVAESRLADRV
                     MGEARGLAA"
     gene            complement(6318..6575)
                     /locus_tag="VIV63_12760"
     CDS             complement(6318..6575)
                     /locus_tag="VIV63_12760"
                     /inference="COORDINATES: protein motif:HMM:NF021559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEY6125515.1"
                     /translation="MNLKTIAIASAVGTLLALGATTASAADEAKEKCLGVAAAGQNDC
                     ATSKHSCAGQAKADKAADEWKYVPKGECEKMGGKIVEKKAM"
     gene            7026..>8519
                     /locus_tag="VIV63_12765"
     CDS             7026..>8519
                     /locus_tag="VIV63_12765"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6125516.1"
                     /translation="MSSHRNRGVSRAVALLCGLSASFATSLVIAQEKPDEKALDEVVV
                     TGSRIARPNLESPVPVTTINSEELFQTGTTSVGDLLNDLPALRSTFSQSNSSRFLGTT
                     GLNLLDLRGLGTQRTLVLVNGRRHVGADILSNAVSPDVNTFPTDLIERIDVVTGGNSA
                     VYGSDAIAGVVNFVLKKDFTGLQFRGQGGLSDEGDASNYYGSILGGMNFMDDRGNIAF
                     NFEYAGQEAWFASERGDYKNQAGFVVVDSDPSGSDGNPDRLFFNDIRFASIANSGSFL
                     LANNAMAPTLTPCGVDPLGARYSCSYIFNSNHELVPQTGQRIGLAPTGNFLGGNGMNN
                     REENLLAIYPDLDRYSLNVFGHLEIAEAFVPFIEAKYVRTESIRYGSPAFFQGPTTGD
                     PRETVRFDNPYLSDQARAVLQQARVNAGFTGGAPGSATGPYIGTSRLALRRNLIEFGP
                     RREDATRETSRIVLGVGGQVSENWNYEVAFNYGQFEEDTIVRGNMNMQ"
BASE COUNT         1621 a         2731 c         2688 g         1479 t
ORIGIN      
        1 gttccttctc agcgttggcc ttctcgagtt cttccgtccg ttcggtgaca cgttcctcga
       61 gatgccggcg gtagctttcc agatcctcga acgcgcgggc atcggcgagc gcgcccgcga
      121 cctgctcgcc caactgccga atcacattca gatcggctgg cgcgtagacg cccgtctgcg
      181 tgtgctgcac gctcaacaag ccgagcacga ttccgtcgtt gacgagtggc acagctaaca
      241 aggatcccgt tggcttctcc gtggactccg cccgttgcac cagctcggtc ggcgcacgac
      301 tccagtcggg gacgagcagc ggctccgcgc gtgagatcag ccagccgaac aatccggtgt
      361 cgacaggttt catgcgcgcc ggcatgcgga cctgctgccg ctcgtgcaat cggatatcca
      421 tcaggcgtcg ctcgcgatcg acgagcacca ggtagagctc gtcgaaccgg aacaacgaac
      481 gggtttcggc gaggatgcgc tcggcgagat cgtcgacacg ccgggcgccc tgcagcttga
      541 gtccggcttc gaacaggcgc gtgagcggac cgcgctcggc ttgcacggga tcgcgatgtg
      601 atccgatgct gttgaagctc agcacgaaca gcagcatcag gcctgcgaac aaccagaaca
      661 ccgccgggcg gtcggtattg aaaagcaccg cgcccaacac gccggccgga acaaagatca
      721 gatccgagag cgcgtatcca ggggtgacga tgcgccgtac ttctcgtccg tcgaagagga
      781 agaacaacat catcaatgcg atgttgacgg cctgcgcggt cagcgccatc gcagcgagag
      841 gcaacacgtc cgtgagcagg agccctttga gcggatgccg gccgtcgcag gcgaggtagg
      901 cataacccgc gagcaacaac atgagtgccg tcatcgaggc attatggatt ccgcgcagcg
      961 cggccacctt gagcgagccc tgactgtaac gacggctgac caacggccag gtcagcgacg
     1021 cgatgccgca gatggacgca gcaacggcgg ggtcgaagac gagcaacatg ccgatctgcg
     1081 gcagccgctc catcgagatc agtccgagac gcggtgcccg caggccgaac tgccacacga
     1141 acagacaggt gccgatgccg agcaacaccg cccaccacgc cggaaactcg gcaccccgca
     1201 acgccagcaa cagcgccccg actgccacgc acgcgagcag aaacgagtag atggacagtg
     1261 cgccatatcg cgagcgcatg cgaaagcact cgattacgcg gtcagaccgc taccgggcca
     1321 ctgtggcacg cgatcggaaa gcgggaaagt gccggtccgg gggaaggcac cgcaaatgag
     1381 acaaatctac gctgcgccgc gagatttcag gccccatacg actcgcgagg cagatcttgc
     1441 agacttgcca cgccgaaaga tggggcttcg acaccacgcg ggttattgca ggcgcgcgac
     1501 gaaatcctgc aggaagacgg ccgcgttcac ctcgagcgcc acctcggagt tgggtcgggc
     1561 gccatcaact ggccgcgtga gaccgtcatc cgcgacttcg atattcagcg acgcgagctt
     1621 gtacggcgtg cgtggattca ccagctccac cgccagcacg tcgaacaacg tcgggtcgtt
     1681 gcccttccag tggttggtgt aacgccagat ctggtcgagc gaggcgagcg tgtcgttcac
     1741 cttcgtgccg cgcgcaaaaa tctgcacgcg cagctcgggt gtcagtttga gatcagccgt
     1801 ggaatcgaga ggcgcgatca gcagcggcac gccggaggtg aacacgatgc gcgcggcctg
     1861 ggcgttcgac ttgatgttcc actcgggctc gggtttcgag cctggagcat ggccgcgata
     1921 gatggctcca cccatgagcg agatggcgcg aatcttcttg ccgatgccag gctccgactt
     1981 caacaacgcg gcgatgttcg tgagttcgcc caccgcgatc agtgtgatct cgccgggacg
     2041 ggcgttgatc tgctggcgca tgaattccac gccaccgtcg gcatgcagag ccggcgacga
     2101 gaacccatcg gcccattcga cctgcttcat gtactgcgtg gcggtggaga ttccggcgta
     2161 caccggcacg ccggcccact tcggtccggc gacctgcagt agtttggcgg caaggcgggc
     2221 tcgcgccacg gcgtcgctcg aaaccgtggt gacaccgagc agttcgagat ccggacgccg
     2281 gatcagcgcg atcaacgcgt aggcgtcgtc gatatcggtg ccgatgtccg tgtcgaatat
     2341 caccggtgcc ggcctggcgg cggacgctac gttcgcgacc gtgaataaca gcaggaacag
     2401 tagcgggatg cgcatggccg ccgagtatag gcgagccctt caaccctgtg cggcgctcga
     2461 ctcccagagg atccgcgcct gggcggcgat gatgtcggga cgctcttcag gccagaacag
     2521 tttcgcgccg gagagcatcc gcacgccgcg cgaattgccg aaactgcggt gcaggtagtc
     2581 tgcgttggac acatcgaaga tcgtgtcgtc gatgccccag acgattcgcg tgggtacgcg
     2641 gcatcgctgc aaggcgggct ggatgccgag caacgcgttg tgaccgagcg ccactgcata
     2701 ccgatgcagc gctgatttgc gttccgccga gttcacgagc ggctcgaagt acatctgcag
     2761 cgcttcatcg gtggggtgcg ccggattcgc gtaacacagg ccgccgatac cgtccttcga
     2821 tcgcgcgagt ccgggatccg cgtgccacgg catcagccac tcatcgacga acctgccctg
     2881 ccgggcgagg tcgatcaccg gcagcatggc cgccggcggg ctctggatct cggtatcgca
     2941 attggtgagc aacaggctgc gcacgcgcgt ggggtaccgc gcggtgaaga gctgggccac
     3001 cgcgccgccg ctgtcgttgg caatcagatc ggcctgcgcg acgccgagtg cgtccagcag
     3061 cgaggccagc atcagcacct gcgaatccgg ccccacgtcc tggttgcggc tcgaccgagt
     3121 gtgtcccgtg ccgaggaagt ccggggcaat acacagcgca tggggtgcaa gccgttcgat
     3181 gacgccgcgc cactgaaatc cattgagcgg aaagccgtgc agcagcaaga ccgcgcgccc
     3241 gcttccgcga gatacgtgcg cgatttcgcc gagaggcgta cgcgtgaact ggcgcgcttt
     3301 cgtgaaacct gcggcatcga acggcgagcc ctcgccggcg ctctccctta tcgcgcacga
     3361 ggcaaccgta ttcaccgcta acaagtgacc ggccagcgcc agcatgtgtc gtcgattgaa
     3421 cgtttgcatg ccacgtattc ggccacgcga acggctcgac ggctgtcgaa ttctttagca
     3481 gtcgcgtgac taaatatttc gacagcgcag gcgctgcgtg cgtggcgaaa tcacgaacat
     3541 gaaaacgaac ccacgactcc tcgcccgata cctcggcgct ccggcgctgg cattggcgct
     3601 cgccaacgca ccagcgtccg atcacctcga tactgccgcg gtcatcgccg atcccgcgag
     3661 cgatattggc gatttgtatg cgtgggcttc ggacggcaag cggctcaatc tcgtgatgac
     3721 gatagtcggc ggaaaattct ccgatcacgt tcgttatgaa tttcacatcg acagcggcac
     3781 gtcgccggat cattccgcag cgactatctc gattgcctgc gaattcaatt cggccttcgg
     3841 ccctgattgc cggctcggag acgtcgatcg cgcgcgcggc gacgccagcg gagaggccgg
     3901 gctcgcaagc gaaaaacgca ggttccgcgt gttcgcgggg ctgcgcgacg atcctttctt
     3961 caacaatgtg cgaggtacac gcgccgcact caacgcggcc ggcgccgcgc tcgcaaacac
     4021 gcgacgaagc gatgcgggtt gtccggcctt cgaccaggcg acgacctcca ggcttctcga
     4081 agagtggcgc cataccgacg gcaagcccgg ccagaacttc ctggagggct ggactaccgc
     4141 ggcgctggtc atcgaagtcg acctcgaggc ggttcgcatc ggcggctctt tgttaggcgt
     4201 gtgggcgact acgacggtgc gcggcgtgac gcccgggcac ccgcgcgtgc tcgacgacgg
     4261 cgttcccatc gaccggatgg gccgggcgtt gaccgccaac gcattgctgg ggaccttcga
     4321 cactcaagag gtcagcaacc ggcgcaagag caagtacaac caggcgcccc gcgcagagtg
     4381 ggcctcgttc gcgccggaca tagccaccac gctcgccatc tacgatggct tcgatggtgt
     4441 gtgtggcaat cagtggatgg ccggtaagca gggcaagccg gcagctcgtt tcgaacggct
     4501 cgccagcatg ctggccgacg atcgcctgtg ggtgaacagc gccgctccgc actgcgcgag
     4561 ctacctggct gtggagctcg aactctcggg cgattgtgga ggccggaccc cgaactacga
     4621 tgccgtcgat gtgttccgtt cgttgctcgt gggtggacgc gtccagggcg tcgacgacgg
     4681 agtagtgcgc gacgaccggc gccattccga atcaggattc ccgtttctcg ccgcgcccgt
     4741 cgcgaagggc gctgactagt ccttcgttga atacaaagcc aagcgccgac accacgcctt
     4801 cctgatcgag accggcgcta ccgatggcgt cccccaacgg agccccggcg ctgagcgcgg
     4861 atagataaga gaagagcggc gccgggagtt ggcggatgtc gatgccatcg atacgcagtc
     4921 ggaccatacc gacctccgga cccagtgatt gattcactgg tggtgcattc accacctgat
     4981 tcgtttccca caccgtgaac accgggtagt cggaagcgag cagcgacagc gaaggctgca
     5041 gcgtgaacac gagatgttcg agttgagcgg gagcgaaggc ggcgagcgcg tcggcggtga
     5101 gcgcggactc gatcctggcg atcgacgcgt gttcgcgagc ccattcgagg cgggcgagat
     5161 cggtcagcca ggcgtagtcg ccgccgtgaa gatgttccag caggaacgcc ggaaagtccg
     5221 cgcccgacca atgaagatcg ccactgcgcg agggaaatcg cgcgcggtac agcgagcaga
     5281 gctggcggaa atagtcgtcg cccacacgcc ggcgcaggac cggatagctg ctttcgagcg
     5341 ccgcgcgaaa ggagagcgcg ctgttgttgc ggtagatcgc aaggttgccc accggcaaaa
     5401 cggggcatgg cgccgaaggg tcccggagcg cggcggcaaa cgcgccctgc aattcaagca
     5461 gcgaggccac gcgcctctcc catcacgcga tccgcgagcc ggctttcggc caccagtgtc
     5521 gccagatcgg gaatgtcggt gtcccattcg atgagcgtgg gctgcgcgcc aaagcggtag
     5581 atggcgcttc gatacaagcg ccacacgtct tcgcacacct tcgagccatg gtcgtcgatg
     5641 aacacgtcgc cgttgcgcgc gtgacccgcg agatggatct cgcccacgcg atcgacggga
     5701 atgccgttga tgaacgcgtt cgcgtcgatg ccgagattgc gcgagctcac gtagatgttg
     5761 ttcacgtcga gcagcagccc gcagccggaa gtcgccgcga ccgcggcgag gaattcccac
     5821 tcgtgcagcg tcgaatgctc gaatgaaacg taggcggata cgttctctat gagaagacga
     5881 gtcccgagga aatcctgggc ctcgccgacc tggcgcgcga gaagcagcgc cgcttcctcg
     5941 gtgtacggca gcggcagaag gtcgttgaag aactcgcctc ccgcgtggcc ccagcaggca
     6001 tgctcggaca ccagcgccgg ctcgaatcgt gtgatggcgc gtttcacgcg cgacaggtgt
     6061 tcgcggtcga cgccttccgc ggagcccagc cccaggccga cgccatgcag actcagcggg
     6121 tagtgggcgc gcgcacgctc gagcgcgcgt acggccgggc cgccgtcgtg gaaatagttt
     6181 tcggtatgcg cttctatcca cccgaccgcc gggcgatcgg tgacgagtgc gtcatggtga
     6241 atggcccgaa ggccgattcc ggcacgcgcc ggaatcggct cccgcacacc tcgtgactcg
     6301 aggcgtggca tgggtgatta catcgccttc ttttcgacga tcttgccgcc catcttctcg
     6361 cactcgcctt tcggcacgta cttccattcg tcagcggctt tgtcggcttt ggcctggccg
     6421 gcgcaggaat gcttcgaagt cgcgcagtcg ttctgaccgg ccgcggcgac gccgagacac
     6481 ttttctttcg cttcatcggc ggcggacgcg gtggtggcgc cgagcgccag cagggtgccg
     6541 acggccgaag caatggcgat ggtcttcagg ttcatgagac tcctccggaa ttttgtcttt
     6601 ggaaatgttt gatcgacggg gaaaggaccc ggccaccgac cgctttattt cgcccacgag
     6661 caggcaaacc aaacataacc ccactcgtcg gacgtgaatg ttcctcgtct gcgcaggact
     6721 cggcaaccgt ttgcttttct gcgacaggtt ctaatgcggg gcaacctgtt ggaattcagc
     6781 cgcttttcgc gctctgacgg ggcgtgacgg tgccgcgcgg agcgctagct attccggcat
     6841 ttgcaaatac ccgcgttccc gtcccacaag cggtttcttt ttttcttccg gttgttatgc
     6901 tccggtcatg gatgcgtgcg tgcaacaaca cgttcgcatc aaggggattc tcaaaaaaat
     6961 cagctgttcc acgcagcgat gcgaacgctg cggtacgaac cgcccactct cttgcaggag
     7021 ggatcatgtc ttctcatcgc aaccgcggcg tcagccgcgc agtggctttg ctttgcggtt
     7081 tatccgcatc gttcgccact tctctcgtaa ttgcccagga aaagcccgac gaaaaggctc
     7141 tcgacgaagt cgtcgtcacc ggttcgcgca tcgcgcgtcc caatctagag tcgcccgttc
     7201 ccgtcaccac gatcaacagc gaagagctgt tccagacggg caccacctcg gtcggcgatc
     7261 ttctgaacga cctgcctgca ctgcgcagca cgttcagcca atcgaactcc agccgcttcc
     7321 tgggtaccac cggtctcaac ctgctcgatc tgcgtggcct cggcacgcag cgcacgctgg
     7381 tgctggtcaa tggccgccgc cacgtgggcg cggacatcct cagcaacgcc gtgtcgcccg
     7441 acgtcaacac gttcccgacc gacctcatcg agcgcatcga tgtcgtgacg ggcggcaact
     7501 ccgcggtgta cggctcggac gccatcgcgg gcgtggtcaa cttcgtgctc aagaaagatt
     7561 tcaccggcct gcagttccgc ggccagggtg gtctgagcga cgagggcgac gccagcaact
     7621 actacggcag catcctcggc ggcatgaact tcatggatga ccgcggcaac atcgcgttca
     7681 actttgaata tgccgggcag gaagcctggt tcgcctccga gcgaggcgac tacaagaacc
     7741 aggctggctt cgtggtcgtc gactccgacc catccggcag cgacggcaat ccggaccgcc
     7801 tgttcttcaa cgacatccgg tttgcgtcga tcgcgaactc cggctcgttc ctgctcgcca
     7861 acaatgcgat ggcgccgacc ctcacgcctt gcggcgtcga tccgctgggt gcccgataca
     7921 gctgctctta tatattcaac agcaaccatg agctggtgcc gcagacaggc cagcgaatcg
     7981 gccttgcacc tacgggaaat ttcctgggcg gcaacggcat gaacaatcgc gaggaaaacc
     8041 ttctcgcgat ctaccctgac ctcgaccgtt acagcctcaa cgtcttcggc catctcgaga
     8101 tagccgaagc gttcgtgccc ttcatcgaag ccaagtatgt ccgcacggag tccatccgct
     8161 acggcagccc ggctttcttc cagggcccga ccacgggtga tccgcgcgaa acggttcgct
     8221 tcgacaatcc gtacctgtcg gaccaggcgc gcgcggtact gcagcaggcc cgcgtgaatg
     8281 caggattcac cggcggcgcg ccggggtcgg ccaccgggcc gtacatcggg acttcacgcc
     8341 tcgcgctgcg caggaacctc atcgagttcg gtccgcgccg tgaagacgcg acccgcgaga
     8401 cgagccgcat cgttctcggc gtcggcggcc aggtgagcga aaactggaac tacgaagtgg
     8461 cgttcaacta cggccagttc gaagaagaca ccatcgttcg cggcaacatg aacatgcag
//
LOCUS       DATDEU010000533         2781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1853197, whole genome shotgun sequence.
ACCESSION   DATDEU010000533 DATDEU010000000
VERSION     DATDEU010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853197"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VIV63_12770"
     CDS             complement(<1..779)
                     /locus_tag="VIV63_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY6125517.1"
                     /translation="MLFAVFCLGLTAALPAQEAPSQGSTSRPRIGLVLSGGGARGAAH
                     IGVLKVLEENRVPVDAIAGSSMGAVVGGLYASGLSAADVERVMTSVDWQDAFRDSPPR
                     SSLNFRRKLEDQNFLVKFPLGIKGGKFRLPRGLVQGQKLTQILRGLTLPVAQIQDFDD
                     LAIPFRAVATDIVTGDRVIIGSGDLTTAMRASLSAPGVFSPVESEGRMLVDGGLSSNL
                     PIDVAREMGVDILIVVDCGFPLLERGKLDSVATVSNQMLAIL"
     gene            854..>2781
                     /locus_tag="VIV63_12775"
     CDS             854..>2781
                     /locus_tag="VIV63_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6125518.1"
                     /translation="MRKLLTLVALLPLAVPAADEMEESYPEDIIVTATRDRSVASYTP
                     ASISRIDTETIAAIGSKHQADVLNRNAGVYIQRGSGAESLGAIRSPVLAGAGACGAFL
                     VAEDSLPIRPTGFCNNNEMFEVNYEQARQIEVLRGPGSSMFGASAVHGVINVITPDIH
                     QLPEFEIGGEAGSDSFTRISAAAGASPSVDGPGFGFYGVATRAPGWRDSSGVDEAKLN
                     LLGDMRVGNGYLRVRAAGTVLNQETAGFIQGYNSYEDEDIARTNPNPEAFRDASSARV
                     SAQWNNRDLREDETVLNLAAIYRRSRMDFLQHFLIGQPLEHNAQTSYLLSGTAVFPTA
                     DFLKTRLTVDAEVADSELTEYQPTPATDGAPPANAIRPAGYHYDYTVDSRTFGATLAL
                     EFTFDPAFTVTAAVRADQTNYDYDNHMIDGNTRDDGTACGAGGCLYSRPADRSDRFDN
                     VSPSLTVAFKPFRTLLYLNVSTGFRPPETTELYRLQRQQNEADLDSENIESFEAGWKH
                     DGDHFSLNGAYYQMKKENVILRETNGFNVSNGATRHRGFEYEARYDARRWSLSLNGTF
                     ARHEYAFSRAIEGGETIVSGNDVDTAPRNLHTLAFDIRLGESDQWRLGADTNYVGKYY
                     LDAANTATYPGHVVENLRL"
BASE COUNT          537 a          940 c          859 g          445 t
ORIGIN      
        1 aggatggcga gcatctggtt ggacaccgtc gccacggaat cgagcttgcc gcgttcgagc
       61 agcgggaagc cgcaatcgac gacgatgagg atgtcgacgc ccatctcgcg cgccacgtcg
      121 atgggcaggt tcgaggacag cccgccgtcg accagcatgc gcccttcact ctccaccggc
      181 gagaacacgc ccggcgccga caggctggca cgcatcgccg tagttaggtc gccgctgccg
      241 atgatgacgc gatcgccggt cacgatgtcg gtggccacgg cgcggaatgg aatggcgagg
      301 tcgtcgaagt cctggatctg ggctaccggc aacgtgagtc cgcgcaggat ctgcgtgagc
      361 ttctgcccct gcaccaggcc acgcggcagg cggaattttc cgcccttgat gccgagcggg
      421 aacttcacga gaaagttctg atcttcgagc ttgcggcgaa aattgagcga gcttcggggc
      481 gggctgtcac ggaatgcgtc ctgccagtcg accgaggtca tcacgcgttc gacgtcggcc
      541 gcggaaagtc cggatgcgta caaaccaccg acgacggcac ccatgctgct gccggcgatg
      601 gcatcgacgg gcacgcgatt ttcttcgagc accttgagca cgccgatgtg agccgcgcca
      661 cgcgcgccgc cgcctgacag gacgagtccg atgcgcggcc tgctagtcga gccctgcgat
      721 ggggcttcct gcgccggcag cgccgcggtc agcccgaggc agaacacggc gaacagcaca
      781 ggttttattg ctctattggg cattcgtagc gtggtaagga tagtcgttcg cagggataat
      841 cgcccggcca aacatgcgca aactcctcac cctggtggcc ttgctgccgc tcgcggtccc
      901 cgcggccgac gagatggaag agtcctatcc cgaggacatc atcgtcaccg ccacccgtga
      961 caggagcgtc gcgtcgtata cgcccgccag catctcgcgc atcgataccg aaaccatcgc
     1021 ggccatcggc tccaagcatc aggcggacgt gctcaaccga aatgccggcg tctacatcca
     1081 gcgcggcagc ggagctgaga gcctgggcgc cattcgttca ccggtgctgg ccggcgccgg
     1141 tgcctgcggt gcgttcctgg tggcggaaga cagtctgccg atccggccga ccggcttctg
     1201 caacaacaac gagatgttcg aggtcaatta cgagcaggcc cggcagatcg aagtgttacg
     1261 cggcccgggg tcgtcgatgt tcggtgccag cgccgtacat ggcgtgatca acgtcatcac
     1321 tcccgacatc caccagttgc cggaattcga aatcggcggc gaggcgggat ctgattcttt
     1381 cacccggata agcgctgctg ccggagcgtc accgagtgtg gacgggcccg gcttcggctt
     1441 ttatggagtc gccacgcgcg ctccgggctg gcgagacagc tcgggcgtcg acgaggcgaa
     1501 gctcaacctg ctgggcgaca tgcgggtcgg taatggctac ctgcgcgtgc gcgccgcggg
     1561 cacggtgctc aaccaggaaa cggcgggctt catccagggc tacaacagct acgaggatga
     1621 agacatcgcg cgcaccaacc cgaatcctga agcgttccgc gacgcttcca gcgcgcgcgt
     1681 gtcggcgcaa tggaacaatc gcgatctgcg agaggacgaa accgtgctga acctcgcggc
     1741 catctaccgc cgctcgcgca tggacttcct ccagcacttc ctgatcggcc agccgctcga
     1801 acacaacgcg cagaccagct acctgttgag cggcaccgca gtgttcccca cggcggactt
     1861 cctgaagacg cgtctcaccg tcgacgcgga agtcgcggac tccgagctca ccgaatacca
     1921 gcccacgcct gccaccgacg gcgcgccccc ggccaacgcg atccgtcccg ccggctatca
     1981 ctacgactac acggtcgact cccgcacctt cggcgcgacg ctcgcgctcg aattcacgtt
     2041 tgaccccgcg ttcaccgtga cggcggccgt ccgcgccgac cagactaact acgactacga
     2101 caaccacatg atcgacggca acacgcggga cgacggcaca gcgtgcggcg ccggcggctg
     2161 cctgtattcg cggcccgcgg atcgcagcga ccgcttcgac aacgtctcgc cgagcctgac
     2221 ggttgcgttc aagcctttcc gtacgctgct gtatctcaac gtctccaccg gattccgccc
     2281 gcccgagacc accgagttgt accggctgca gcgccagcag aatgaagccg atctcgacag
     2341 cgagaacatc gagtccttcg aggccggctg gaaacatgac ggcgaccact tctcgctgaa
     2401 tggcgcctac taccagatga agaaggaaaa cgtgatcctg cgcgagacca acggattcaa
     2461 cgtgagcaac ggcgcgacgc gccaccgcgg cttcgaatac gaagcgcgtt acgatgccag
     2521 gcgctggagc ctctcactca acggaacctt cgcgcgccat gaatatgcgt tctcgcgcgc
     2581 gatcgagggt ggcgagacca tcgtgagcgg caacgacgtc gacaccgcgc cccgcaacct
     2641 gcacaccctg gcgttcgaca tccggctcgg cgaatccgac cagtggcgtc tcggcgccga
     2701 tacgaattac gtcggcaagt actacctcga cgccgccaac acggcgacat atccgggaca
     2761 cgtcgtcgaa aacctgcggc t
//
LOCUS       DATDEU010000534         5602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_978705, whole genome shotgun sequence.
ACCESSION   DATDEU010000534 DATDEU010000000
VERSION     DATDEU010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978705"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VIV63_12780"
     CDS             complement(<1..715)
                     /locus_tag="VIV63_12780"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125519.1"
                     /translation="MIPLALVGMSYREAPSAVRAALAAFDSDAGGPTKQLLDAGEITG
                     VVRIESCARVEWLLASERPSWAAELFSAALIGAAENPDHIRPRVRHGAAAVGGLLRVA
                     AGLDSVAQGEHAIGRQVLRAFEQSHEAGTVCRSLHVAWRGVGDLLNRARRILPQGRTG
                     GVQALVIQRLAGVARNETVAVFGLGEIGQAMLRSLSDAGFTAVESFNRASMRAFESFV
                     RRAANVIVCSGAPSGWLHLP"
     gene            complement(723..1757)
                     /gene="hemE"
                     /locus_tag="VIV63_12785"
     CDS             complement(723..1757)
                     /gene="hemE"
                     /locus_tag="VIV63_12785"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY6125520.1"
                     /translation="MHSRERFLAALRGDALDRPPVWLMRQAGRYLPGYRALRAEHSFW
                     DVCQNPELSTRAALEPLDRYKLDAAIVFSDILVVPLAMGLGVTFGPGEGPRIAKPLRT
                     GADFDAWQLDGVLDRIAYLPRAVAHLKETLKDRYGILGFAGAPFTLFAYCVEGGGSDD
                     FREARTLMHRDPVLARRALNTIADVVAGLLLAQVDAGADAVQLFDSWGGLLAPDEYRD
                     FALPALQRITAKLNARKVPTLLFVRGGHHLLPLLGESGVTGFSLDWRTEWQVARDMYP
                     THLLQGNIDPVLLLGSADVIRSRTRELLETMKKTTGGRSCILNLGHGILPGTPPETVA
                     ALVETAVEFN"
     gene            1867..2637
                     /locus_tag="VIV63_12790"
     CDS             1867..2637
                     /locus_tag="VIV63_12790"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY6125521.1"
                     /translation="MTEFALPYTWIVTKSQPHADSLVIQLRRQGFRALAISCIEHEWH
                     DWPELRRIGAAGAAVLFVTSRAAAARVDVPQGVMVAAISPTTSATLQARGISVGLSAP
                     GGVRELAQAVHDSAAIPPGADVFYPTSDAALRQPEHLAAVATLSQRLRVHTQAVYSTV
                     APANLAQELTALRTADSPTPPPLGFCFWSPSSIEHFAAARGFELPPGPVVLIGGSTLR
                     CWRETAPAEWRRAYQHDAETPLEWSLRFLERGETPGGS"
     gene            complement(2642..3100)
                     /locus_tag="VIV63_12795"
     CDS             complement(2642..3100)
                     /locus_tag="VIV63_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY6125522.1"
                     /translation="MTDSPLEAELTTKVLASLNKILELELAGVVRYMHYSFMIFGHNR
                     IPIVAWLRDQSNESNAHAVMAGEHITAFGGHPSLSIGGLLETHKHDVNEILVEAHEHE
                     KEGLDAYYALLKLVEGKSILLEEYARDQIAAEEMHLADIRKMMRKPGSIK"
     gene            complement(3221..4903)
                     /locus_tag="VIV63_12800"
     CDS             complement(3221..4903)
                     /locus_tag="VIV63_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125523.1"
                     /translation="MKHPISRLLSIVAAALLLSRAVPAAETFDVVIDVNAARAGQDVT
                     PIWRFFGADEPNYATMKDGRQLLGELGLLKKDDVYFRAHNLLSSGDGTPAMKWGSTNV
                     YTEDAAGNARYDWRIVDEIFDAYLSNGVRPFVQLGFMPQALSTAPASVPYKHSWRPGF
                     DYNLISGGWGYPPRDYDRWSELCFELTRHLVQRYGEAEVQRWWFETWNEPNGPAYWKA
                     SREEFYKLHDYAIAGVRRALPSARVGGPHTAGHGGAYMEGFLDHITQGKNHATGEQGT
                     PTDFLAFHAKGAPNFVDGHVRMGIQAHLATIDTGFRMIAARPTLAGKPIIIGESDPEG
                     CAACQGPRFGYRNGTMYSSYTAASFARKHELATRHRVNLQGALTWAFEFEDQPYFAGF
                     RQLASNGIDMPVLNVFRMFSLMQGRQLPAKSSRQVPLDDILARGVGGAPDVGVMATRR
                     DDTIAVLVWHYHDDDVAGPSAAVTLNVTGLRRGTPTVTHYRIDQQHSNSYAAWQRMGS
                     PIAPDKASYDSLRAAARLSTLDAAATSVARVGGSVQLAFELPRQGVSLLLIR"
     gene            complement(4958..>5602)
                     /locus_tag="VIV63_12805"
     CDS             complement(4958..>5602)
                     /locus_tag="VIV63_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6125524.1"
                     /translation="PFKANQYDVGFEYYFSDVNYVSAAAFRTEISRFIINTAAPEVID
                     GLTYNITRPINGVDEVTIDGFELGGQFAFDFLSGAWKNFGVMANATWSDDKGFTGTNL
                     LTGELLPFPGVSELSYNASVYYETEKFSVRTAYNWRESWLLTPAGRGGLPEFNDDYGT
                     LDLSASFSPNEHFTVFADVVNLLDEQKVEFNNPFRRIGNETFGKRFFLGVRAKF"
BASE COUNT          988 a         1813 c         1828 g          973 t
ORIGIN      
        1 ccggcaaatg cagccatccg gagggagcgc ccgaacagac gatgacattc gccgcccggc
       61 gcacgaacga ttcgaacgcg cgcatgctgg cgcgattgaa gctctcgacc gcggtgaatc
      121 ccgcgtcgga aagcgaacgc aacattgcct ggccaatctc acccagcccg aagaccgcca
      181 cggtctcgtt gcgcgccacg ccggcgagcc gctggatcac cagtgcctgc acaccgcccg
      241 tgcggccctg cggaagaatg cgccgcgccc gattgagcag atctcccacg ccgcgccacg
      301 cgacatgcag gctgcggcac accgtgccgg cttcgtggga ctgttcgaag gcgcgcagca
      361 cctggcgtcc gatggcgtgt tcaccctgcg ccactgaatc gaggccggca gccacgcgca
      421 gtagtccacc gactgccgcg gcgccgtggc gcacgcgcgg ccggatgtga tcgggattct
      481 ccgcggcgcc gatgagcgcg gcgctgaaca gttccgcggc ccacgagggt cgctccgaag
      541 cgagcagcca ttcgacgcgc gcgcaacttt cgatacgcac cacgcccgtg atctcgcccg
      601 catcgagtag ttgcttcgtc gggccgcccg cgtcggagtc gaacgcagcg agcgccgcgc
      661 gcaccgccga aggcgcctcg cgataactca tgccgacgag agccagcggg atcatggcgg
      721 tgtcagttga attccacggc ggtctcgacc agcgccgcca ccgtttccgg cggcgtgccc
      781 ggcaggatgc cgtgaccgag attcaggatg cagctgcggc cgcccgtcgt cttcttcatg
      841 gtttcgagca gctcgcgcgt gcgcgaacga atgacatcgg cgctgcctaa caacaacacc
      901 ggatcgatgt ttccctgcag caggtgcgtg gggtacatgt cacgggccac ctgccattcg
      961 gtgcgccagt cgagcgagaa accggtgacg ccgctttcgc cgagcaacgg caacaggtgg
     1021 tgtccaccgc gcacgaacag caacgtgggc accttgcgtg cattcagttt cgcggtgatg
     1081 cgctgcaggg ccggcagcgc gaaatcgcgg tattcatcgg gcgcgagcag cccgccccac
     1141 gagtcgaaca gctgcacggc atcggcaccg gcgtcgacct gcgccagcag caggccggcg
     1201 acgacatcgg caatcgtatt cagtgcgcgg cgcgccagca ccggatcgcg atgcatcagc
     1261 gtgcgcgctt cgcggaaatc gtcgctgccg ccgccttcca cgcagtaggc aaacaacgtg
     1321 aacggcgcgc cggcgaaacc gaggattccg tagcggtcct tgagcgtctc tttcagatgc
     1381 gccacggcgc gcggcagata ggcgatgcgg tcgagcacac cgtcgagttg ccacgcgtcg
     1441 aagtctgcac ccgtgcgcaa cggcttggcg atgcgcggac cttcgccagg accaaaggtg
     1501 acgccgagac ccatggcgag gggcaccacg agaatgtcgg agaacacgat ggcggcgtcg
     1561 agcttgtagc gatcgagcgg ttcgagcgcg gcgcgcgtgg acagctccgg gttctggcag
     1621 acatcccaga agctgtgttc ggcgcgaagt gcgcgataac ccggcaagta acgccccgcc
     1681 tggcgcatca accacaccgg aggacggtcg agcgcatcgc cgcgcagcgc cgccagaaat
     1741 cgctcacgcg aatgcatcgt ggataaactc cattgaaatc aaaagaaagc gtacggcggg
     1801 cccgcgcaca aactgtcaca gctgtgtcac gccggaagcg gcgcgggtgc cccggggcta
     1861 acattcatga cggagttcgc gttgccctat acctggatcg tcacgaagtc acagccgcat
     1921 gccgacagcc tggtcatcca gctgcgccgg cagggtttcc gcgcgctcgc gatttcctgc
     1981 atcgaacacg agtggcacga ctggccggag ctgcggcgca tcggtgccgc cggcgccgcg
     2041 gtgctgttcg tgaccagccg tgcggcggcg gcccgcgtcg acgttccgca gggcgtcatg
     2101 gtggcggcga tatcacccac cacctcggcg acacttcagg cacgaggtat cagcgtgggg
     2161 ctttccgcgc ccggcggcgt tcgcgagctg gcacaggccg tacacgattc ggccgccatt
     2221 ccgcccggcg ccgacgtttt ctatcccacc agcgatgcgg cgttgcgcca acccgaacat
     2281 ctggctgccg tggcgacgct gtcacaacgc ctgcgcgtac atacccaggc cgtgtattcg
     2341 accgtggctc ccgcgaatct cgcgcaggag ctcaccgcgc tgcgaacggc cgactcgcca
     2401 acgcctccac cactgggctt ctgcttctgg agtccgtcga gcatcgagca tttcgcggcc
     2461 gcacgcggct tcgaactgcc tccggggcct gtcgtgctca tcggcgggtc gacgctgcgg
     2521 tgctggcggg aaaccgcgcc ggccgagtgg cggcgcgcgt atcagcacga cgcggaaacc
     2581 ccgctcgagt ggtcgctgcg ttttctcgaa cgcggcgaaa cacccggcgg tagttagctg
     2641 gctacttgat cgagcccggc ttgcgcatca tcttgcggat gtcggcgaga tgcatttctt
     2701 ccgccgcgat ctggtcgcgc gcgtattcct cgagcaggat gctcttgcct tccacgagct
     2761 tgagcagcgc gtagtacgcg tcgaggcctt ccttctcgtg ttcgtgcgcc tcgacgagga
     2821 tctcgttcac gtcgtgtttg tgcgtctcga gcaggccacc gatgctgagc gacggatgcc
     2881 cgccgaaggc cgtgatgtgt tcgccggcca tgacggcgtg cgcgttcgat tcattcgact
     2941 ggtcgcgcag ccacgcgacg atcgggatgc ggttgtgacc gaagatcatg aagctgtagt
     3001 gcatgtagcg cacgacgccc gcgagttcga gttcgaggat cttgttcagg ctggcgagga
     3061 ccttcgtggt cagctcggct tcaaggggtg agtcggtcat ggcgtaatcc tcgtgcggct
     3121 tcaatcgtgg cgctgaatgt tagtgcagcc gggcgcggtt gtcagacatg cgaatgccaa
     3181 tgcgaatatc tcgcatccgg cacgccgccg cgacttctag ctaccggatg aggagcagcg
     3241 acacgccttg ccgcggcaac tcgaaagcca gttgtacgga acctccgaca cgcgccacgg
     3301 aagttgccgc cgcgtcgagc gtcgaaagcc gtgcggcggc gcgcaacgag tcataggacg
     3361 ccttgtcggg cgcaatcggc gagcccatgc gttgccatgc cgcataggaa ttgctgtgtt
     3421 gctggtcgat gcgatagtgc gtcacggtcg gcgttccgcg ccgcaaaccc gtgacgttca
     3481 atgtgacggc cgccgacggc cccgccacgt cgtcgtcgtg gtagtgccag acgagcacgg
     3541 cgatcgtgtc gtcccgccgc gtcgccatga cgcccacgtc gggcgcgccg cccacgccac
     3601 gggcgagaat gtcgtcgagc ggtacctgcc gcgagctttt cgcgggcaac tgccggccct
     3661 gcatgaggct gaacatgcgg aacacgttga gcaccggcat gtcgatgcca ttgctcgcga
     3721 gctggcgaaa ccccgcgaag tacggctggt cttcgaattc aaatgcccag gtcagggcgc
     3781 cctggaggtt cacgcgatgg cgcgtggcga gttcatgttt gcgcgcgaaa ctggcggcgg
     3841 tgtagctcga gtacatggtc ccgttgcgat agccgaagcg tggcccctgg caggccgcgc
     3901 agccttccgg atcggactcg ccgatgatga tgggcttgcc ggccagcgtc ggccgcgcgg
     3961 cgatcatcct gaacccggtg tcgatggtcg cgaggtgcgc ctggataccc atgcgtacgt
     4021 ggccatcgac gaaattcggc gcgcccttgg cgtgaaacgc gagaaagtcc gtcggcgtgc
     4081 cctgctcgcc cgttgcgtgg ttcttgccct gcgtgatgtg gtcgagaaat ccttccatat
     4141 acgcgccacc atggccggcg gtgtgcgggc caccgacgcg cgcggacggc aacgcgcggc
     4201 gtacaccggc aatcgcatag tcgtgcagct tgtagaactc ttcgcgcgac gctttccagt
     4261 aggcggggcc gttaggttcg ttccaggtct cgaaccacca gcgttgcact tccgcttcgc
     4321 cataacgttg cacgagatgc cgcgtgagtt cgaaacacag ctcagaccag cggtcgtaat
     4381 cgcgcggcgg atatccccat ccaccgctga tgaggttgta gtcgaatccc ggtcgccagg
     4441 aatgtttgta gggcacggac gcgggcgcgg tgctcaacgc ctggggcatg aatccgagct
     4501 gcacgaatgg ccgcacgccg ttcgatagat aggcgtcgaa tatttcgtcg acgatgcgcc
     4561 agtcgtagcg cgcgttgccg gccgcatcct cggtgtagac gttggtgctg ccccacttca
     4621 tcgccggcgt gccgtcgcct gacgacagca ggttatgcgc gcggaagtag acgtcgtcct
     4681 tcttgagcag accgagctcg ccgagcagtt gccggccgtc cttcatggtg gcgtagttgg
     4741 gctcgtcggc gccgaagaag cgccagatcg gcgtcacatc ctggccggcg cgcgccgcat
     4801 tcacatcgat gacgacgtcg aaggtttcgg cagcgggaac tgcgcgggaa agcagcagcg
     4861 ccgcggcaac gatcgacagg agacgcgaga tcggatgttt catcggacca ttatggggcg
     4921 gacagaccat cgagtctgcc cgccccggtt caggacatca gaacttcgcg cggacaccca
     4981 ggaagaagcg cttgccgaaa gtctcgttgc cgatgcgacg gaacgggttg ttgaactcga
     5041 ccttctgttc gtcgagcaag ttcacgacgt cggcgaacac cgtgaagtgc tcattcgggc
     5101 tgaagctcgc cgacaggtcg agcgtgccgt agtcgtcatt gaactctggc agaccgccgc
     5161 gaccggccgg cgtcagcaac cagctctcac gccagttgta cgcggtgcgc acgctgaact
     5221 tctcggtctc gtagtacacg gaggcgttgt agctcagctc ggagacaccc gggaacggca
     5281 gcagttcgcc ggtcagcagg ttggtgcccg tgaagccttt gtcgtcggac cacgtggcat
     5341 tggccatcac gccgaagttc ttccacgcgc ccgacaggaa gtcgaacgcg aactggccgc
     5401 ccaactcgaa gccgtcgatg gtcacttcat ccacgccatt gatgggacgc gtaatgttgt
     5461 acgtcagacc atcgatcact tccggtgccg ccgtgttgat gatgaaccgc gaaatttccg
     5521 tgcggaacgc cgcggcggac acgtagttca cgtcggagaa gtagtactcg aagccaacgt
     5581 cgtattgatt ggccttgaac gg
//
LOCUS       DATDEU010000535         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_978769, whole genome shotgun sequence.
ACCESSION   DATDEU010000535 DATDEU010000000
VERSION     DATDEU010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978769"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1473)
                     /gene="purD"
                     /locus_tag="VIV63_12810"
     CDS             complement(172..1473)
                     /gene="purD"
                     /locus_tag="VIV63_12810"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY6125525.1"
                     /translation="MKILIVGSGGREHALAWKCAQSSRVSEVLVAPGNGGTATEARVR
                     NVNVAAEDIPGLIALAKAEKVGLTIIGPEGPLVMGVVDAFATAGLRCFGPRKGAAQLE
                     GSKAYTKEFLKRHGIPTAAYATFTRDSFDADWVRKQRTPIVVKASGLAAGKGVVIVDT
                     AEEAIATAQAMFGGQFGSAGDQVVIEEFLPGEEASFIVMADGKNIVTMATSQDHKRLR
                     DADAGPNTGGMGAYSPAPVVDRAMHERIMREVIEPSIRGLAKDGTPYTGFLYAGIMVH
                     PDGTPNVLEYNCRFGDPETQPIMMRLKSDLVELCEAALDHRLDSVHAEWDSRAALGVV
                     LAAEGYPDSVRKGDEIAGLGAAAKLPGKVFHAGTRADGGKVFTHGGRVLCAVGLGDSV
                     SAAQKQAYGLVDAIRWQGMQCRRDIGYRAIERERAAKPTRS"
     gene            complement(1483..>2948)
                     /gene="purH"
                     /locus_tag="VIV63_12815"
     CDS             complement(1483..>2948)
                     /gene="purH"
                     /locus_tag="VIV63_12815"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY6125526.1"
                     /translation="TGGTARALRDAGLAVIDVSAYTGFPEIMDGRVKTLHPRVHGGLL
                     GRRGVDEAVMAKHDIPPIDLLVVNLYPFAQTVAKPDCTYADAIENIDIGGPAMVRAAS
                     KNHESVTVIVDPADYGQVLGELDANDGATSIDTRSSLAAKAFAHTAKYDTMVSNYLLG
                     RESEDAASFPATLPLVYEKIQDLRYGENPHQKAAFYRELGLTGTCVASATMLQGKELS
                     FNNIADTDTAVECARVFNEPACVIVKHANPCGVATGVSLADAYDRAYRTDPTSAFGGI
                     IAFNRELDAETAIAITGRQFVEVIAAPSVSAEAVVVLAGKANVRVLVLGPLSSKTPTE
                     LEYRSVVGGLLVQSRDTALAQPDSFKIVTEKRPTPTQYADLWFAWRVCKYVKSNAIVF
                     AREGMTIGVGAGQMSRVYSTHVAALKAKDEGLEVEGSSMASDAFFPFRDGIDAAAKYG
                     IKAIVQPGGSKRDDEVIAAANEHGMTMVFTGMRHFRH"
BASE COUNT          451 a          988 c          969 g          540 t
ORIGIN      
        1 ctgatcccgc ggggttgcgc cgcgagccag gcccgcgcga catcgaggcc gggcgcggag
       61 aactgccatt cctgctcggt gttctcttcc atttttttgg ggccaccgcg cggcgcgcga
      121 attcccgggg cactgtatgc ccggcagccg gacggcgtcc atcgcgagga ctcaggagcg
      181 ggttggtttt gccgcccgct cccgttcgat ggcgcgatag ccgatatccc gccggcattg
      241 catgccctgc cagcggatgg cgtccacgag cccataagcc tgcttctgcg ccgccgagac
      301 gctgtctccc aggccgacgg cacacaacac ccggccgccg tgcgtgaaaa ccttcccgcc
      361 gtcggccctc gtgcccgcgt gaaacacctt gccgggtagt ttggccgcgg cgccgagccc
      421 cgcgatctcg tcgcccttgc gcacgctgtc cggatagcct tcggccgcca gcaccacgcc
      481 gagcgcggca cgcgagtccc actccgcgtg gaccgagtcc agccggtgat cgagcgccgc
      541 ttcgcacagc tccaccaggt cgctcttgag ccgcatcatg atgggctgcg tttccggatc
      601 tccgaaccgg cagttgtact cgagcacgtt gggcgtgccg tcggggtgca ccatgatgcc
      661 ggcgtacagg aagcccgtgt acggcgtgcc gtctttcgcg aggccgcgaa tggaaggctc
      721 gatgacctcg cgcatgatgc gttcgtgcat ggcgcggtcc accacgggcg cgggtgaata
      781 cgcgcccatg ccgccggtgt tggggccggc atccgcatcg cgcagccgct tgtgatcctg
      841 cgacgtcgcc atggtcacga tgttcttgcc gtcggccatg acgatgaagc tggcttcctc
      901 gcccggcagg aattcctcga tgaccacctg gtcgcccgcg gagccgaact gaccgccgaa
      961 catggcctgg gcggtggcga tggcttcctc tgccgtgtcg acgatcacca cacccttgcc
     1021 ggcggcgagc ccgctcgcct tgacgacgat cggcgtgcgc tgctttcgga cccagtccgc
     1081 gtcgaagctg tcgcgcgtga acgtggcgta agcggcggtg ggaatgccgt gacgcttgag
     1141 gaattccttg gtgtaggcct tggagccttc gagctgcgcg gctcccttgc gaggcccgaa
     1201 gcagcgcagt ccggcagtgg cgaacgcatc gactactccc atcaccagcg gcccctcggg
     1261 cccgatgatg gtgaggccga ctttctctgc cttggcgagc gcaatcagcc ccgggatgtc
     1321 ctcggcggcg acattgacgt tgcgcacgcg cgcctcggtg gccgtgccgc cattgccggg
     1381 agccacgagc acttcggaaa ccctggatga ctgcgcgcac ttccacgcga gcgcatgttc
     1441 gcgcccgccg gaacccacga tcaggatctt catggccgtg gctcagtgcc ggaagtggcg
     1501 catgccggtg aacaccatgg tcatgccgtg ttcattggcc gcggcgatga cttcatcgtc
     1561 acgcttgctg ccaccgggct gcacgatggc cttgatgccg tatttggccg cggcgtcgat
     1621 gccatcgcgg aacggaaaga aggcatccga ggccatggag ctgccttcga cttccaggcc
     1681 ttcgtctttc gccttgagcg cggccacgtg cgtggagtac acgcgcgaca tctgcccggc
     1741 gcccacgcca atcgtcatgc cctcgcgcgc gaacacaatg gcgttcgact tcacgtactt
     1801 gcacacgcgc caggcgaacc acaggtccgc gtattgcgtc ggcgtcggcc gcttttccgt
     1861 gacgatcttg aaggagtcgg gctgcgccag cgcggtatcg cggctctgca ccagcaggcc
     1921 gccgacgacg ctgcggtatt cgagctcggt cggcgtcttg ctcgagagag gcccgagcac
     1981 cagcacgcga acgttcgcct tccccgcgag caccaccacg gcttccgcgg ataccgaagg
     2041 cgcggcgatg acttcgacga actggcggcc cgtgatggcg atggccgttt ccgcgtcgag
     2101 ctcgcgattg aacgcgatga tgccgccgaa cgcggacgtg ggatcggtgc gataggcgcg
     2161 gtcataggcg tcggccagcg atacgcccgt ggcgacgcca caggggttgg cgtgcttgac
     2221 gatgacgcag gcgggttcgt tgaacacgcg agcgcattcc acggcggtgt ccgtgtccgc
     2281 gatattgttg aaagagagtt ccttgccctg gagcatggtg gcgctggcca cgcatgtgcc
     2341 ggtgagaccg agctcgcgat agaacgccgc cttctgatgc gggttctcgc cataacgcag
     2401 gtcctgaatt ttctcgtaca ccagcggcag ggttgccgga aacgaggcgg cgtcttcgct
     2461 ttcccgcccc agaagatagt tggacaccat ggtgtcgtac ttcgcggtgt gggcgaacgc
     2521 cttcgcggcg agactcgatc gcgtgtcgat actggtggcg ccatcgttgg cgtccagctc
     2581 gccgagtacc tggccgtagt cggccggatc gacgatgacc gtcaccgatt cgtgattttt
     2641 ggacgccgcg cgcaccatcg ccggcccgcc gatgtcgatg ttttcgatgg cgtccgcgta
     2701 cgtacagtcc ggtttcgcga ccgtctgcgc aaacgggtac agattcacga ccaggaggtc
     2761 gatcggtgga atgtcgtgct tggccatgac ggcctcatcg acgccgcgac ggcccagcag
     2821 gccgccatgg acccgcggat gcaatgtctt gacgcgcccg tccatgatct ctggaaagcc
     2881 cgtataggcg ctgacgtcaa tcacggcgag ccccgcgtcc cgcagggccc tggcggtgcc
     2941 gccggtgc
//
LOCUS       DATDEU010000536         4237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_978957, whole genome shotgun sequence.
ACCESSION   DATDEU010000536 DATDEU010000000
VERSION     DATDEU010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978957"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VIV63_12820"
     CDS             <1..433
                     /locus_tag="VIV63_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6125527.1"
                     /translation="IAIRSEFPTAKVIILTTYGGDVLAERALKAGAYAYVLKGLVRKE
                     LLETIRAVHAGQKRIHPDVATELASHVGEEALSEREIQVLKLVAAGNSNREIGEHLHI
                     TEQTAKAHMKRIVAKLGANDRTHAVTLAMNRGFLVSAPPKG"
     gene            573..866
                     /locus_tag="VIV63_12825"
     CDS             573..866
                     /locus_tag="VIV63_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125528.1"
                     /translation="MPLWIVGTAPLWASSDSLMWFRILGCLTGALILAGLWTPLAASC
                     HAILQGLLAFAGEMFDPTHLVLALVGASLAMLGPGAWSIDARLYGRKRIELGK"
     gene            complement(896..1792)
                     /locus_tag="VIV63_12830"
     CDS             complement(896..1792)
                     /locus_tag="VIV63_12830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6125529.1"
                     /translation="MDTRRLRYFVQIVDSGSITRAAAVSGVAQPALSQQLAILENDLK
                     VKLLDRSVSGVKPTAAGHILYARAQAILRQLDDLREAVHREVRPLSGTVVVGMPPSML
                     SRFGFPLIGKVCTQHPEMHLQIREEGSLVLDELLTGGKVEISISATRPGGAVTGEEIL
                     TEVIVLMYPPAFELPENATLAELARLPWVLPRRPNAIRTLVDAAFAAHNLSPNVVVEI
                     DSLHSALETVRRGYGVGATTMGAIEEDLRAGTLKARALGDAPLMRSMYLARRRSPGLT
                     PAAEFVSGLLRDIAAEVAQANA"
     gene            1878..3164
                     /locus_tag="VIV63_12835"
     CDS             1878..3164
                     /locus_tag="VIV63_12835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6125530.1"
                     /translation="MTEDPGVTIRKTRLRLLTPLLALLFLSTVDRANISFAALQMNAE
                     LGLTAETYGFGVSIFFVGYILVQWPSLWLLQRIGMRRWVFACAGTWGLAAAGLAFVHS
                     AESFYALRLLLGIAEGGFAPGVMFYLAQWIPSRSRAAAISTFMLAVPISAIFGGPLAG
                     WLMSVDNPVQWAGWRWMLLIEGLPTILLAIAALWLLPDRPRDARWLSAPEQRWIENEI
                     SREKAAHGTSTPAMSPLRNGRLWIASACWFGLMAGANGMLYWLPQIIRHLSAQSTDLQ
                     IGFISALPWIAVGAGMVVNAWHSDRAQERYWHVGLAALAAAAFIALTPLLGTGALALS
                     ALMFAGFAMGAAQGTFWTLPPLLLHPASLATGFALINMSGNLAGLVIPTFIGWVRART
                     GSFDGPVFVLAGLSVLAAVAVALLRSRRPIEAPYSR"
     gene            3231..>4237
                     /locus_tag="VIV63_12840"
     CDS             3231..>4237
                     /locus_tag="VIV63_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY6125531.1"
                     /translation="MPEDEYDPTADETFESTYAVFADLRARCPVAHSNAFDGFWAVTR
                     YQDIHDILMQPDLYITSVRNVVPGSSATGRRPPLHLDPPDHTPYRKAIDRALGAARVA
                     GIEDTTRRIARSLMQSLVAMREADFIEHFSSPLPAAVFGEWLGLTEQQTGVLWKTARA
                     YVKAWESFDKSSVSVAAAQLAAMAADVIAERRLAPRDPELDPTSSLLAARDNEGNPFP
                     EALLAGCVRQVLVVGLVAPPILLGSIVVHLSRDPALQQQLRADPSLIPDAVEEFLRLY
                     TPYRGFARSARREVELHGRRIQPGEPIALVYASANRDESVFPEAGEFRLRRPNIRQHL
                     A"
BASE COUNT          680 a         1391 c         1379 g          787 t
ORIGIN      
        1 gattgccatc cgcagcgagt ttcccaccgc caaggtcatc atcctgacca cctatggcgg
       61 cgatgttctc gcggagcgcg ccctcaaggc gggcgcgtac gcctacgtgc tcaagggcct
      121 ggtgcgaaag gaactgctcg aaaccattcg cgccgtccat gccggccaga aacgcattca
      181 cccggatgta gccaccgagc ttgcgtccca tgtcggcgaa gaggctcttt cggagcgcga
      241 gatccaggta ttgaaactcg tggccgccgg caactccaac agggaaatcg gcgagcacct
      301 gcacatcact gaacaaaccg cgaaggcgca catgaaacgc atcgtcgcga aactcggcgc
      361 caacgatcgc acgcacgccg tgaccttggc gatgaaccgc ggtttcctgg tgagcgctcc
      421 cccaaagggg tagccggcaa actacttttt cgagggtcgc gcacggcggc gatgcccttc
      481 aggctccgtg agagcccatg caacgactat tctccacgtt tccaaacggg cggccgggat
      541 acggtttgct gctgttacga ctaacctgcg gaatgccgct gtggatcgtc ggaacggcac
      601 cgctgtgggc gtcatccgat tcactcatgt ggtttcgcat actcggctgt ctgaccggcg
      661 cattgattct cgcgggactc tggactccgc tcgccgcgtc gtgccacgca attctgcaag
      721 gcttactggc attcgcggga gagatgttcg acccgacaca tctggtgctg gcattggtcg
      781 gcgccagcct cgcgatgctc ggccccggag cctggtcgat cgacgcgcgg ctgtatgggc
      841 gcaagagaat cgaactgggc aagtgaatcc gccgactacg acggcactcg ccggctcatg
      901 cattcgcctg cgccacttcc gcggcgatat cgcgcagaag tccgctcacg aactcggcgg
      961 cgggcgtgag cccgggtgaa cgccggcggg cgagatacat ggagcgcatg agcggcgcat
     1021 cgccgagcgc cctcgccttc agcgttccgg cgcgaagatc ttcttcgatg gcgcccatgg
     1081 tcgtggcgcc gacaccgtaa cctcgccgca cggtctcgag cgcgctgtgt agcgaatcga
     1141 tctcgaccac cacgttcggc gacagattgt gggccgcgaa cgccgcatcg acgagcgtgc
     1201 ggatggcgtt cggtcgacgc ggcagcaccc agggcagccg cgcgagctcg gcgagcgtcg
     1261 cgttttcggg gagctcaaac gccggcggat acatgagcac gatgacttcg gtgaggattt
     1321 cctcgccggt gaccgcgccg ccgggccgtg tggcggaaat ggatatctcg acttttccgc
     1381 cggtcagcag ttcatcgagc acgaggctgc cttcttcgcg gatctgcaga tgcatttccg
     1441 gatgctgggt acacaccttg ccgatgagcg ggaacccgaa acgcgaaagc atgctgggcg
     1501 gcatgccgac caccacggtt cccgacagcg gccgcacttc gcggtggacc gcctcgcgca
     1561 ggtcgtcgag ctgccgaagg atcgcctgtg cccgcgcata gaggatgtgt ccggccgcgg
     1621 tgggtttcac gcccgacacg gaccggtcga gcaacttcac cttgagatcg ttctcgagaa
     1681 tcgcgagctg ctggctcagc gcgggctgcg ccacgccgct caccgccgcg gcgcgcgtga
     1741 tgctgccact gtcgacgatc tgtacgaagt aacgcagccg gcgggtatcc ataggcgtgc
     1801 atcataagag cggcttatgt ggggaaccag aggtgatgtt tccgccgccg gcagtcgctc
     1861 tctatgatcg agcgcgaatg actgaagatc cgggcgtaac catcaggaaa acgagactgc
     1921 ggctgctcac gccgctgttg gcgctgctgt tcctcagcac cgtggaccgg gcgaacatca
     1981 gtttcgcggc gctgcagatg aacgcggaac tcggcctgac cgcggaaacc tacggcttcg
     2041 gcgtcagcat cttcttcgtg ggttacatcc tcgtgcagtg gcccagcctc tggttgctgc
     2101 aaaggatcgg catgcggcgc tgggtgtttg cctgcgcggg cacctggggt ctggcggccg
     2161 ccgggctggc attcgtccac tcggccgaat ctttctacgc gctgcgcctg ctgctcggca
     2221 tcgcggaggg tggcttcgcc cccggcgtca tgttctacct ggcgcagtgg attccgtcgc
     2281 gctcgcgagc cgccgccatc tccactttca tgctcgccgt tcccatctcg gcgatcttcg
     2341 gcggaccgct ggccggctgg ctgatgagcg tcgacaaccc tgtccaatgg gccgggtggc
     2401 ggtggatgct gctgatcgaa ggcttgccga ccattcttct cgcgatagcg gcgttgtggt
     2461 tgctgccgga ccggccacgc gacgcgcgtt ggctgtccgc gccggagcag cgctggatag
     2521 aaaacgagat ctcgcgtgaa aaagccgcac acggcacgag cacgccggcg atgtcgccac
     2581 tgcgaaacgg ccgcctgtgg atcgccagcg cctgctggtt cggtctcatg gccggcgcga
     2641 acgggatgtt gtactggctg ccgcagatca ttcgccatct ctccgcgcag agcaccgacc
     2701 tgcagatcgg attcatctcc gcattgccgt ggatcgcggt gggcgcgggc atggtcgtga
     2761 acgcctggca ttccgaccgc gcgcaggagc ggtactggca cgtgggactc gcggcgctgg
     2821 ctgccgcggc tttcatcgcg ttgacgccgt tgttgggtac gggcgccctg gcgctgtccg
     2881 cgctcatgtt cgcgggcttc gcgatgggcg cggcgcaggg aacattctgg acgttgccgc
     2941 cgttactgct gcatccggca tcgcttgcga ccggcttcgc gctgatcaac atgtccggca
     3001 acctcgcggg actggtcatt cccacgttca tcggctgggt gcgcgcgcgc accggttctt
     3061 tcgatggtcc ggtgttcgtg ctcgccggtc tgtcggtgct cgccgcggtg gcggtggcgc
     3121 tgcttcgttc gcgtcggccc atcgaggcgc cttatagccg gtagatcgaa ccagtgttac
     3181 ccgctcgtgc gcagccaacc gtacattcgg gctcccgcac cggagcccgc atgccggaag
     3241 acgaatacga tccgaccgcc gacgaaactt tcgagtccac ctacgcggta ttcgccgacc
     3301 tgcgggcacg atgtccggtc gcgcacagca atgcgttcga cggcttctgg gcggtgacgc
     3361 gttaccagga tatccacgac atcctgatgc agccggatct ctacatcact tccgtccgga
     3421 acgtggtgcc gggctcatcg gcaaccggcc ggcgacctcc gctgcatctg gatccgccgg
     3481 accatactcc ctatcgcaag gccatcgacc gtgcgctcgg ggctgcgcga gtcgccggca
     3541 tcgaggacac gacgcgccgc atcgcccgtt cgctcatgca gtccctggtg gcgatgcgcg
     3601 aggcggattt catcgaacat ttctcgagtc cgctgcccgc cgcggtgttc ggcgagtggt
     3661 taggcctcac cgaacagcag accggcgtgt tgtggaaaac ggccagggcc tacgtgaagg
     3721 cctgggagtc gttcgacaag agcagcgtgt ccgtcgccgc ggcccagctc gccgcgatgg
     3781 ccgccgacgt gatcgcggaa cggcggctcg cgccgcggga cccggagctc gatcccacga
     3841 gttcgctgct cgccgcgcgc gacaacgagg gaaatccttt ccccgaagcg ctgctcgccg
     3901 gttgcgtgcg ccaggtgctg gtcgtcggcc tggtggctcc gcccatcctg ctaggcagca
     3961 tcgtcgtgca cctgtcacga gacccggcgc tgcagcagca gttgcgcgcc gatccctcac
     4021 tcattcccga tgccgtggaa gaattcctgc ggctgtacac gccctatcgc ggtttcgcgc
     4081 gttccgcgcg gcgcgaagtg gagttgcacg ggcggcgcat tcagccgggc gagccgatag
     4141 cactggtgta cgcctcggcg aatcgggacg agtctgtttt tcccgaggcc ggcgagttcc
     4201 ggctgcgccg gcccaacatc cgtcagcacc tggcatt
//
LOCUS       DATDEU010000537         3190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1707908, whole genome shotgun sequence.
ACCESSION   DATDEU010000537 DATDEU010000000
VERSION     DATDEU010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707908"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VIV63_12845"
     CDS             <1..291
                     /locus_tag="VIV63_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125532.1"
                     /translation="AAHTDVGAYLVGLWGLPNTIAEAIAYHEDPSQSPNAGFGLAGIV
                     HVADRLAHRPDIADPHSPDLGLNVAYLKAQGRLERWNEWREAYSAITQKAPE"
     gene            345..926
                     /locus_tag="VIV63_12850"
     CDS             345..926
                     /locus_tag="VIV63_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6125533.1"
                     /translation="MEKSKLLLVDDEPNLTSALVRSLDRSQFEIFTADSAQKGLMILA
                     GNDIDVVVSDERMPGMTGSQFLTEVRKQWPNTIRMILSGQADLEAAVRAINEGEVYRF
                     LLKPCHPKELQLTILQGLQHKKLVAQSRRLLQEHQKTQNLLEELEKANPGITKIEMDE
                     DGAILMDDSDEGTEDVQSLLDDLEREMSKVARR"
     gene            complement(923..1897)
                     /locus_tag="VIV63_12855"
     CDS             complement(923..1897)
                     /locus_tag="VIV63_12855"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY6125534.1"
                     /translation="MYSRIAGTGSYLPELIVTNGDLEKRMDTTDEWIRSRTGIERRHI
                     AAEGQTTVDLAEVAARRAIEAAGVTPAEIDFIVFGTTTPDLVFPNCGTLLQARLGARG
                     CPAFSVETACSGFIYALSIADKFVKAGEAKCALVVGAETLSRITNWSDRNTAVLFGDG
                     AGAVVLKASAEPGIMSTHLHSDGAYKDLLYVDSGVSKGFNIDPTTNKVDIGIKMAGSE
                     VFKVAVTKLGGAVDEALAHNGLEKSAVNWLIPHQANIRIIGAMAKRLDLPMERVIVTV
                     QDHGNTSTASVPLALDTGVRDGRVKRGDLLLLEAFGGGFTWGSALVRF"
     gene            complement(1907..2890)
                     /gene="plsX"
                     /locus_tag="VIV63_12860"
     CDS             complement(1907..2890)
                     /gene="plsX"
                     /locus_tag="VIV63_12860"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY6125535.1"
                     /translation="MPSIAVDVMSGDCGAPECIPGALLALTTDTALELILVGDPTVIE
                     PALAHVPPAVRSRVSVHPAATVVGMSEHPREAIRRRKDSSMRIAIDLVKEGRAGAMIS
                     AGNTGALTAMSHFVLKTVGPVERAPIMSSVPARNGGHTHILDLGANTKATALQLAQFA
                     TMGSIVARDVFGITTPRVGLLNIGEEDTKGHELIRASGVNYLGFVEGNHIFDENVDVV
                     VTDGFTGNVALKAMEGLAKLIAEQLRTEFSRNITSKLSGLLARPVLRRVGEKLDPRRY
                     NGACMVGLTAVVVKSHGSADSVAFAQAVTTAATAMRRGLPAAIAKALSPDN"
     gene            complement(2927..3121)
                     /gene="rpmF"
                     /locus_tag="VIV63_12865"
     CDS             complement(2927..3121)
                     /gene="rpmF"
                     /locus_tag="VIV63_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY6125536.1"
                     /translation="MPVQKSRKTPSKRGMHRSHDALSKPALSVDPQSGETHLRHRITP
                     DGFYRGKRVIEKPAEVETEE"
BASE COUNT          586 a         1007 c         1036 g          561 t
ORIGIN      
        1 gccgcgcaca ccgacgtggg cgcctatctg gtcggcctct ggggcctgcc gaacaccatc
       61 gccgaagcga tcgcttatca cgaagacccg tcgcagtcgc ccaacgcggg cttcgggctc
      121 gccggcatcg tgcacgtcgc cgaccggctg gcacaccgcc ccgacatcgc cgacccgcac
      181 tcgcccgatc tcggactcaa cgttgcgtac ctgaaagccc agggacgttt ggagcgctgg
      241 aacgagtggc gcgaagccta ttcggcgatc acgcagaaag cgcccgaata agcgcacgga
      301 cacaacttct cgacgatcaa ggcgattgaa taaaggtacc gtttatggaa aagagcaaac
      361 tactgttggt cgatgacgag ccgaacctga cctcggcgct ggtgcgcagc ctcgatcgct
      421 cgcagttcga gatattcacg gccgactccg cgcagaaggg tctgatgatc ctcgccggca
      481 acgacatcga cgtagtggtc tctgacgaac gcatgcccgg catgaccggc agccagttcc
      541 tcaccgaagt gcgcaagcag tggcccaaca ccattcgcat gatcctctcc gggcaggcgg
      601 acctggaagc cgcggttcgc gccatcaacg agggtgaggt gtatcgcttc ctgctcaagc
      661 cctgccatcc caaggaactg cagctgacca tcctgcaggg cctgcagcac aagaagctcg
      721 tggcccagag ccgccggctt ctgcaggaac atcagaagac ccagaacctg ctcgaggaac
      781 tcgagaaggc gaaccccggc atcaccaaga tcgagatgga cgaagacggc gccatcctga
      841 tggacgacag cgacgaagga acggaagacg tgcagtcgct gctcgatgat ctcgagcgcg
      901 agatgtcgaa ggtcgcgcgc cgctagaagc ggacgagtgc cgagccccag gtaaagccgc
      961 ccccgaaggc ttccagcagc agcaggtcgc cgcgtttgac gcggccatca cgcacgccgg
     1021 tgtccagcgc cagcggcacg gacgccgtgg aggtgttgcc gtggtcctgc acggtgacga
     1081 tgacccgctc catcggaaga tcgagccgct tggccatggc gccgatgatg cggatgttcg
     1141 cctggtgcgg gatcagccag ttgaccgcgg acttttccag gccgttgtgc gcgagcgctt
     1201 catcgaccgc gccacccagc ttggtgaccg cgaccttgaa cacttcactg ccggccatct
     1261 tgatgccgat gtcgaccttg ttggtggtcg ggtcgatgtt gaagcccttg gagacgccgc
     1321 tgtcgacgta cagcaagtct ttgtacgcgc catcggaatg caggtgcgtg ctcatgatgc
     1381 cgggctctgc ggaagccttg agcaccacgg cgcccgcgcc gtcgccgaac aacaccgcgg
     1441 tgttgcgatc ggaccagttg gtgatgcgcg agagcgtctc ggcgccgacc acgagcgcgc
     1501 acttggcctc acccgccttc acgaacttgt cggcgatgga taacgcgtag atgaatccgc
     1561 tgcacgccgt ttcgaccgaa aacgcggggc agccgcgcgc accgaggcgc gcctgcagca
     1621 gcgtgccgca gttggggaac acgagatcgg gcgtggtggt gccgaatacg atgaagtcga
     1681 tttcggcggg cgtgacgccc gcggcctcga tggcccggcg agccgcgact tcagccaggt
     1741 cgacggtagt ttggccttcc gccgcgatgt gccgacgttc gataccggtg cgcgagcgga
     1801 tccactcgtc ggtggtatcc atccgtttct cgaggtcgcc gttcgtaacg atcagttccg
     1861 gaaggtagga ccccgtaccg gcaatgcgtg aatacatgcg accccttcag ttgtcaggcg
     1921 agagcgcctt ggcgatggcc gcgggcaatc cgcgacgcat cgcggtggca gcggtggtca
     1981 ccgcctgggc gaatgcgacg ctgtccgcgc tgccgtggct ttttaccacg actgcggtca
     2041 gtccgaccat gcaggcgccg ttgtagcgac gcggatccag cttctcgccg acgcgccgca
     2101 gtaccgggcg ggctagcaac ccggaaagct tgctggtgat gttgcgggaa aattccgtgc
     2161 gcaactgctc ggcgatgagt ttggcgaggc cttccatcgc cttgagcgcg acgttgccgg
     2221 tgaagccgtc ggtgaccacc acgtcgacgt tctcgtcgaa gatgtgattg ccttcgacga
     2281 agccgagata gttcacgccg ctggcgcgga tcagctcgtg tcccttggtg tcttcctcgc
     2341 cgatgttgag cagtccgaca cgcggtgtcg tgatgccgaa cacgtcgcgc gcgacgatcg
     2401 aacccatggt ggcgaactgc gcgagctgca gcgcggtcgc cttggtgttt gcgccgaggt
     2461 cgaggatgtg cgtgtggccg ccgttacgcg cgggaaccga cgacatgatg ggcgcgcgtt
     2521 caacgggccc cactgtcttc aacacgaagt gggacatggc cgtcagcgca ccggtgttgc
     2581 cggcgctgat catggcgccc gcccgccctt ccttcacgag gtcgatggcg atgcgcatgg
     2641 aggagtcctt gcgccggcga atggcttcgc gcggatgctc ggacattccc accacggtgg
     2701 ccgccggatg cacggacacg cgcgaacgaa ccgccggcgg cacgtgagcc agcgcgggtt
     2761 cgatgacggt ggggtcgccc acgagaatca actcgagggc ggtatcggtg gtgagggcga
     2821 gcagcgctcc gggtatgcac tccggcgcac cgcagtcgcc actcatcaca tcgacggcga
     2881 tcgacggcat gcgttacgag cggaggcgaa cgcccccgcc ccgcaactat tcttcggtct
     2941 cgacttcggc cggtttttcg atgacgcgtt tgccgcgata aaaaccgtcg ggagtgatgc
     3001 ggtgacgcag gtgggtttca cccgactgcg gatcgacgga cagggcgggc ttcgacagcg
     3061 catcgtgcga acgatgcata ccgcgcttgg acggcgtttt acgacttttc tgaacgggca
     3121 tggaaccaat cctcttgggc gactactact tgcgtttcaa cagttcgccc aactgtgcaa
     3181 acggtttttg
//
LOCUS       DATDEU010000538         6924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1708099, whole genome shotgun sequence.
ACCESSION   DATDEU010000538 DATDEU010000000
VERSION     DATDEU010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708099"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VIV63_12870"
     CDS             complement(<1..676)
                     /locus_tag="VIV63_12870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY6125537.1"
                     /translation="MIASMTGFARRELAGSWGTLTCELRSVNHRYLEPGFRLPEELRP
                     LESDLRALLSKNLKRGKVDCTVHLRSMQTAERELRLDEAALTRVAAALKQVARTVPGS
                     TIDAVEVLRWPGVIETASQDSDALLGAGRAVFQQTLDELGAMRLREGKRLGELIEQRC
                     AGLGMLVTQVRARLPEIQTRVRTRLHERVAELLATVDRERVEQEIVLQLQRLDVAEEL
                     DRLTGHI"
     gene            753..1502
                     /locus_tag="VIV63_12875"
     CDS             753..1502
                     /locus_tag="VIV63_12875"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY6125538.1"
                     /translation="MRIEYAEVSLLGCRTDNQDRVSVAVSEHSALLVVIDGMGGHSDG
                     AKAAETAIKSLVEGFWHTPQPILDPLGFLHLTLGRAHEEVAKLGANLPLELRPRATCA
                     VCLVQNGSAFWAHIGDSRVYLLRRGKVFKRTRDHSHVEFLLREGVITADQAISHPMRN
                     FVECCLGGEPFLPEMSIGMREPLEANDILLVCSDGLWGGLDDGDIGGAFPPARAPLRD
                     ELVRLAERSVSVVGAGSDNTSAAVVRWIGNG"
     gene            1502..2227
                     /gene="rph"
                     /locus_tag="VIV63_12880"
     CDS             1502..2227
                     /gene="rph"
                     /locus_tag="VIV63_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY6125539.1"
                     /translation="MAGRPSGRAPDELRAIKFTRRFTQHAEGSVLVEFGSTRVLCTAT
                     VEEGVPGFLRGKNQGWVTAEYGMLPRATHTRSPREAAKGKQSGRTQEIQRLIGRSLRA
                     VTNMAALGERTVTLDCDVLQADGGTRTAAITGCYVALADACDGLIAKRLISASPLHGQ
                     VAAVSVGICSGVPVLDLDYAEDSCAETDMNVVMNNGGGFIEVQGTAEGHAFRRHELDA
                     LLNLASTGIAKLFQMQTEVRTAA"
     gene            2248..2853
                     /gene="rdgB"
                     /locus_tag="VIV63_12885"
     CDS             2248..2853
                     /gene="rdgB"
                     /locus_tag="VIV63_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY6125540.1"
                     /translation="MRGQRVVLATGNPGKLRQFSELLDASGLDLVRQSDFGIEPPPET
                     GSTFLENSLLKARNAARLTGLPAIADDSGIEVDALGGRPGLYSARYAGEGASDEQNLR
                     KMLLELENVQPPRTARYRSIIVFLSRPDDPAPLIGEGVWEGAIATAPRGTGGFGYDPI
                     FIPRGDTRTAAELPVAERNELSHRGQAARAFLAQMNTRARG"
     gene            2850..4010
                     /gene="hemW"
                     /locus_tag="VIV63_12890"
     CDS             2850..4010
                     /gene="hemW"
                     /locus_tag="VIV63_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY6125541.1"
                     /translation="MKTPPLALYAHFPWCVQKCPYCDFNSYTLRDELPEQRYVDTLLR
                     DLDAQVGDLKARPLTSIFLGGGTPSLFAPAAIGRVIDHARLRLGFAGDIEITLEANPG
                     TIERGRFAEYRAAGVTRVSLGAQSFDERQLKLLGRIHSADETRRAAEELHAAGLPNFN
                     LDLMYALPGQTADEAEADLRAALALKPAHLSQYHLTIEPGTVFAAAPPTQPTDEIVED
                     MLQRCLTSLEESGFEQYEVSGYARAGAKCDHNLNYWTFGDYLGIGAGAHGKLSFPERG
                     FAERTQQTREPRRYLAAEPGALVRRPIPEAELPFEFAMNGFRLVHGFDDEMFESRTGL
                     PIAVLEQALAPQVERGLVERGANRWRATAKGFRFLNDILVELLPATRASPSP"
     gene            4356..4568
                     /gene="rpmE"
                     /locus_tag="VIV63_12895"
     CDS             4356..4568
                     /gene="rpmE"
                     /locus_tag="VIV63_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY6125542.1"
                     /translation="MKADTHPEYKEIAVTCTCGNAFKTRSTLGHDLQVEVCASCHPFY
                     TGKQKILDTAGRVDKFRKKYAAATTR"
     gene            4817..6193
                     /locus_tag="VIV63_12900"
     CDS             4817..6193
                     /locus_tag="VIV63_12900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY6125543.1"
                     /translation="MGSWEGEKKTTQKHHQEIVKALGLDDDQATQEYVSQVGQRLALA
                     SDLPNEEFKFFVIDDEGINAFTTGCCNVYVNRGLLVNLNSEAELAGVLGHEIGHITAR
                     HPARRQTRGMASQLGAMAAAILTGSNAIGQLANIGAAAWMSGYGRENEMEADRLGMKY
                     MVKAGYNPESIGNVFDMFQAGEKFERQRAAEEGREPRLYHGVFSSHPSPDQRAVQAAK
                     GAANIKDAPPEGWLERHDEYLKTINGIAYGTSKAQGVVRDNRFYHADMGITVAFPRGW
                     TIENQRDRLIAFTPKKDAVIQVMIDAKPDKQAPREFLLTKLKGASTFKGEPITTADGE
                     GYMVVARSGSPIDNGTGPVRWAVVYRGQSAFIFAGASRSALDGVPEVDGLIKSTTLTL
                     RGLKPSEFPLAEPYRIKVVKATDATKLSDYAADMPEDKFKKETLELINAMYPNKRPKS
                     GQLFKIVE"
     gene            6311..>6924
                     /locus_tag="VIV63_12905"
     CDS             6311..>6924
                     /locus_tag="VIV63_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY6125544.1"
                     /translation="MPDTKLELLDPATGKKAVLPVRSGTVGPSVIDIGPMTKEFGAFT
                     YDPGYGATAAVESKITYIDGEAGVLTHRGYPIEQLAEKSSFIEVCYLLLNGELPNKAQ
                     LETFEHSIRNHTMINESLLRFFGGFHHNAHPMAMVSAVVASMSAFYHDSMDIHNPRHR
                     EIFAHRIVAKLPTIAAAAYKHSLGQPFVYPRNDLSYCANMLHMF"
BASE COUNT         1337 a         2229 c         2126 g         1232 t
ORIGIN      
        1 cgatgtgtcc ggtgaggcgg tcgagctctt cggccacgtc caggcgctgc agctgcaaga
       61 cgatttcctg ctcgacccgc tcgcgatcga cggtggccag cagctcggca acgcgttcgt
      121 gcaggcgcgt gcgcacgcgc gtctggatct ccggcaggcg cgcgcgcacc tgggtgacca
      181 gcatgccgag gccggcgcat cgttgttcga tgagctcgcc cagccgcttg ccttcgcgca
      241 ggcgcatcgc gccgagctcg tcgagcgtct gctgaaagac ggcccggcca gcgcctaaca
      301 atgcgtccga atcctgagac gcggtttcga tgacgccggg ccagcgcaac acctcgacgg
      361 cgtcgatggt gctgccggga accgtacgcg cgacctgctt gagggccgcc gcgacgcgtg
      421 tcagcgccgc ttcgtcgagg cgcagctcac gttccgcggt ctgcatgctg cgcaggtgca
      481 ccgtgcaatc gaccttgccg cgcttgaggt tcttgctgag gagcgcccgc aggtccgatt
      541 cgaggggccg gagctcttcg ggcaggcgaa accctggctc gagatagcga tgattcacgc
      601 tgcgcaactc gcaggtcagg gttccccagc tgccagcgag ctcgcgtctc gcgaaaccgg
      661 tcatgctggc aatcatgcgt gtaagttctc ggcttaaggg gagcggggtg cggtaaagtg
      721 cgcggcagtt tatcagaccc ctcagccaag ccttgcgcat cgaatacgcc gaagtcagcc
      781 tgctaggttg tcgcaccgac aaccaggatc gcgtatccgt cgcggtctcg gagcattccg
      841 ccctgctcgt cgtcatcgac ggcatgggag gtcattccga tggcgccaag gccgccgaaa
      901 ccgccatcaa gtctctggtc gaaggcttct ggcacacgcc gcaacccatt ctcgatccgc
      961 tcggattcct gcatctgacg ctcggccgcg cgcatgagga agtcgcgaag ctcggcgcca
     1021 acctgccgct cgaactgcgg ccgcgcgcga cctgcgccgt gtgcctggtg cagaacggca
     1081 gcgcgttctg ggcgcacatc ggtgacagcc gcgtctatct tctgcggcgc ggaaaggtgt
     1141 tcaagcgcac tcgcgaccac agccacgtcg aattcctgct gcgcgaaggc gtcatcacgg
     1201 ccgaccaggc gatcagtcat ccgatgcgca acttcgtcga gtgctgcctg ggcggtgaac
     1261 ccttcctgcc cgagatgtcg attggcatgc gcgagccgct ggaggccaac gacatactgc
     1321 tcgtctgttc cgacggcttg tggggcggcc tcgatgacgg cgacatcggt ggggcatttc
     1381 cgccagcgcg cgcgccgttg cgcgacgaac tcgtgcgcct ggccgaacgc tcggtaagcg
     1441 tcgtcggcgc cggcagcgac aacacttcag ccgcggtcgt tcgctggatc ggtaacggct
     1501 gatggctggc aggcccagtg ggcgcgcacc ggatgagctg cgcgccatca aattcacgcg
     1561 ccggttcact cagcacgcgg aaggctccgt gctcgtcgaa ttcggcagca cacgcgtgtt
     1621 gtgcacggca accgtcgaag aaggtgttcc cggatttctt cgcggcaaga accagggctg
     1681 ggtcacggcc gagtacggca tgctgccgcg cgccacccac acgcgttcgc cgcgtgaggc
     1741 ggccaagggc aagcagagtg gccgcacgca ggaaatccag agattgatag gtcgcagcct
     1801 gcgggcggtg accaacatgg cggcgctcgg cgaacggacc gtcaccctcg actgcgacgt
     1861 cttgcaggcc gacggcggca cgcgcaccgc ggcgatcacg ggttgttatg tggcgctggc
     1921 cgacgcctgc gatggactga tcgcgaagcg gctcatttcc gcatcaccgc tgcatggaca
     1981 ggtcgccgcc gtgtcggtgg gcatctgctc gggagtgccg gttctcgacc tcgactacgc
     2041 cgaagattcc tgcgccgaga ccgacatgaa cgtcgtcatg aacaacggcg gcggattcat
     2101 cgaagtgcag ggaaccgcgg aaggtcatgc gtttcgccgc cacgagctcg atgcgctgct
     2161 caatctcgca tccacgggga tcgcgaagtt gttccagatg cagaccgagg tgcggacggc
     2221 cgcctgaggc ggccgacgca tacgcgcatg cggggtcagc gcgtggtgct cgctaccggc
     2281 aatcccggaa agttgcgcca gttttccgag ttgttagacg cgtcgggcct ggacctggtg
     2341 cggcaatcgg atttcggcat cgaacctcct ccggaaaccg gcagcacatt tctcgagaat
     2401 tccctgctga aggcgcgcaa tgccgcgagg ctcacgggtc tgcccgcgat cgccgacgat
     2461 tcgggcatcg aagtcgacgc gctcggcggg cgaccgggtc tctactccgc gcgatacgcc
     2521 ggggagggcg cgagcgacga gcagaacctg cgcaagatgc ttctcgaact cgagaatgtg
     2581 cagccgcccc gtaccgcgcg atatcgcagc atcatcgtat ttctcagtcg ccccgacgat
     2641 cccgcgccgt tgataggtga gggcgtctgg gaaggcgcga ttgccacggc tccccgcggc
     2701 accggtggct tcggctacga cccgatattc attccgcgcg gcgacacgcg aaccgccgcc
     2761 gaattgccgg tcgcggaacg taacgagctc agccatagag gtcaggcggc gcgcgcgttc
     2821 ctcgcccaga tgaatacccg ggcacgcgga tgaaaacgcc accgctcgcg ttgtatgcgc
     2881 actttccgtg gtgcgtgcag aagtgcccgt attgcgattt caattcgtac accctgcgcg
     2941 acgagttgcc ggagcagcgt tacgtcgaca cgctgctacg cgatctcgat gcacaggtcg
     3001 gggacctgaa agcgcgaccg ctcaccagca tcttcctggg tggcggtacg cccagcctgt
     3061 ttgcgccagc ggccatcgga cgcgtcatcg accacgcacg ccttcgtctg ggttttgccg
     3121 gcgatatcga gatcacgctc gaggcgaacc cgggaaccat cgaacgcggc cgtttcgccg
     3181 aatatcgagc agccggagtg acccgcgtgt cactcggcgc gcagagtttc gatgagcggc
     3241 agttgaaact gctcggtcgt atccattccg ccgatgagac gcgacgcgcg gccgaggaac
     3301 tccacgccgc cgggctcccg aatttcaatc tggacctgat gtatgcgctg cccggccaga
     3361 ccgccgatga agcggaggca gacttgcgcg cggcgctcgc gctgaagccc gcgcatcttt
     3421 cgcagtacca cctgaccatc gagccaggca ctgtcttcgc ggcagctcct cccacacagc
     3481 ccaccgatga aatcgtcgag gacatgctgc agcgatgcct gacctcgctc gaagagtccg
     3541 gatttgagca atacgaggtg tccggctacg cgcgtgctgg cgcgaaatgt gaccacaacc
     3601 tcaactactg gacctttggc gattacctcg gcatcggcgc aggcgcacac ggcaaacttt
     3661 cgtttccgga gcgtggattc gcggagcgca cgcagcagac acgcgagccc cggcgctacc
     3721 tcgccgccga acccggcgca ctggtcagaa ggccgattcc ggaagccgag ctgccattcg
     3781 agttcgccat gaacgggttt cgactcgtcc acggcttcga cgacgagatg ttcgaatcgc
     3841 gaacaggcct gcccatcgca gttctcgagc aggcgctggc gccccaggtg gagcgtggcc
     3901 tggtcgaacg gggcgcgaat cgctggcgcg cgacggccaa aggcttccga tttctcaacg
     3961 acatactcgt tgaattgctg ccagcgaccc gggcatctcc gtccccctga tcgccgatgc
     4021 cccgttgacg ggcgctttta ttcacagatg ccccgtgacg ggcacgtaaa caatgggatt
     4081 acggaaggtt tcaatcctgt tgcactacaa acacactgta atcgactgat ttataagcgt
     4141 ttaacgtaat ggtcattatt tgaccaagct aacggttttg cgcggatcga agtggttttg
     4201 gagccgggaa cagcagctac ccacagagtt atccacaaat tctgtggata acgcggcctt
     4261 gctatttggg cttgagaacc cacaactggc ccgctagact gcgcgccctt tttcttgtga
     4321 ccggccggca aagaccgccc ggatagagag aacccatgaa agccgatacg catcctgaat
     4381 ataaagaaat cgccgtgacc tgcacctgcg ggaacgcctt caagacgcga tcgactctgg
     4441 gacacgacct ccaggtcgaa gtctgcgcca gctgccatcc gttctacacg ggcaagcaga
     4501 agatcctgga caccgccggc cgcgtggaca aattccgcaa gaagtacgct gccgcgacga
     4561 cgcgctaacg aactttccgg gcattcgatg ccactcaaga aggcgcctcc gggcgccttt
     4621 ttgctttgcc gaccggtggt tcccggaact tgttgtcgtg acgccatatc gtagttaaat
     4681 acgagctcgg cccgtccgcg gaagagagct acatacaatg aagcgcattc ggctatttcc
     4741 ccttctcatc gcggccacct gcgcgttcct ttccagctgt ggaagcaacc cggcgacggg
     4801 cggaaagaac gtagtcatgg gttcgtggga gggtgaaaag aagaccaccc agaagcacca
     4861 ccaggaaatc gtcaaggcgc tgggcctcga tgacgatcag gccacgcagg aatacgtgag
     4921 ccaggtaggg caacgactgg cgctcgccag tgatcttccc aacgaagaat tcaagttctt
     4981 cgtcatcgac gatgaaggca tcaacgcctt tacgaccggc tgctgcaacg tctacgtgaa
     5041 ccgtggactg ctcgtgaatc tcaactcgga agcggagctc gcgggtgtgc tcggccatga
     5101 aatcggccac atcacggcgc ggcaccctgc ccggcgacag acgcgcggca tggcgtcgca
     5161 actcggcgcg atggccgcgg ccatcctcac cggctcgaac gcgatcggtc agctggccaa
     5221 cattggcgct gctgcctgga tgagcggcta tggccgcgag aacgagatgg aagcggatcg
     5281 gctcggcatg aagtacatgg tgaaggccgg gtacaacccg gaatcgatcg gcaacgtgtt
     5341 cgacatgttc caggcgggcg aaaaattcga gcgtcagcgt gccgcggaag aaggccgcga
     5401 gccgcgcctc tatcacgggg tcttctcgag ccacccgagc ccggatcagc gcgcggtcca
     5461 ggccgcgaag ggtgcggcga acatcaagga cgcgccgccc gaaggctggc tcgagcgcca
     5521 tgatgaatat ctgaagacca tcaacggcat cgcctacggc acgagcaagg cgcagggcgt
     5581 cgtgcgcgac aaccgcttct atcacgcgga tatgggaatc accgtcgcgt ttccgcgcgg
     5641 ctggaccatc gagaaccagc gcgatcgcct gatcgctttc acgccgaaaa aagacgcggt
     5701 catccaggtc atgatcgacg cgaagccgga caagcaggcg ccgcgtgaat tcctgctcac
     5761 caaactcaag ggcgcttcca cgttcaaggg tgagccgatc accaccgccg acggtgaggg
     5821 ttacatggtc gtggcccgca gcggatcacc catcgacaac ggcacgggtc cggtgcgctg
     5881 ggcagtcgtc tatcgcgggc agagcgcttt catcttcgcg ggcgccagcc gctcggcgct
     5941 cgacggtgtg cccgaggtcg atggcctgat caaatcgacc acgctgactc tgcgcgggct
     6001 caagccatcc gagtttcccc tcgccgagcc gtatcgcatc aaggtggtga aggccacgga
     6061 tgcgaccaag ctttccgact acgcagctga catgcccgaa gataaattca agaaagaaac
     6121 tctcgagctg atcaacgcga tgtatccgaa caagcggccg aaatccgggc aactgttcaa
     6181 gatcgtcgag taacgattca cgacgggcgc tcgtgcgccc tctcgttgcc ttgattccat
     6241 tcgcgggccg tgtaaggctc gtgtcgattt ttcccgtgcc actcgacgcg ccttgcaggg
     6301 cctcttcgta atgcctgaca caaaactcga acttctcgat ccagcaaccg gcaagaaggc
     6361 cgtgcttccc gtccggtccg gaaccgtcgg cccctccgtc atcgacatcg ggccgatgac
     6421 caaggaattc ggcgccttca cgtacgatcc cggttatggt gcaaccgctg ccgtcgagag
     6481 caagatcacc tatatcgacg gcgaggccgg cgtgctcacg catcgcggct atcccatcga
     6541 acaactcgcc gagaagagct cgttcatcga ggtctgctat ctactgctga atggcgaatt
     6601 gccgaacaag gcgcagctcg agaccttcga gcacagcatt cgcaatcaca cgatgatcaa
     6661 cgagtcgctg ctgcgtttct tcggcggctt ccaccacaac gcgcacccca tggccatggt
     6721 gtcggcggtg gtggcttcga tgtcggcctt ctatcacgac tcgatggaca tccataaccc
     6781 gcgccatcgc gagatcttcg cgcaccgcat cgtcgccaaa ctgccgacga tcgccgcagc
     6841 cgcctacaaa cactcgctcg gccagccttt cgtctatccg cgtaacgacc tcagctactg
     6901 cgccaacatg ctccacatgt tctt
//
LOCUS       DATDEU010000539         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1126981, whole genome shotgun sequence.
ACCESSION   DATDEU010000539 DATDEU010000000
VERSION     DATDEU010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126981"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VIV63_12910"
     CDS             <1..243
                     /locus_tag="VIV63_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in the processing of the 5'end of 16S
                     rRNA; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY6125545.1"
                     /translation="GRGFVRTPETVCHEIFREIVRQSRQFQSRELLILAHQNVIDRLL
                     DDESAVLAELEHQVGRPIRLQVEALYGFDQYDVVLA"
     gene            323..>2223
                     /locus_tag="VIV63_12915"
     CDS             323..>2223
                     /locus_tag="VIV63_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125546.1"
                     /translation="MLVTFCLLLLGTLGVVFTRVVAARVPEQRATLEKLIADRTGLAV
                     RFDNVHFAWNLDGTSAVFERVELTDPARGRVRVVAPELRVEFDTWDFLRHHQFSLGHV
                     TLASPDIEIIGDALETPVADVRAPKGKANAQKAAPAQEDEATLVRRFAAWAELMPNGR
                     IEVEGARVHLRRRGDRAARNTFTLSQAVLIRGASNFNAFGTMLLSQDVGQSLFLSAKL
                     EGLGTPAGVTGDLRLIARRVFLDKVPLIDAAGRGTLDATLHLENGLVHTAKWQASARE
                     LEFDDERARFDHFTVSGKLERDADDFLLQFADLQITRGARLERAPRLSARISIEPGTT
                     RIARTTLRSERVPFMAAEFITRLLAPQAAHRISEFPGGWVPTGGELRTVRFDSGPKSG
                     QDFAAWTFNAQIAGGDLERARDHARVSGVAAMLRLSKGEVAFDFDPANAVNVRAPGEL
                     PPRALNLGGRLVMLSTGDAVARFETFSARSADSSLALDGQWNEGQPLAVTVSNIDRAM
                     VLDGWRLFANDAAAPGWLGEIGSGVVTEGKLNLLTLRDADGHRAVNWQRSSGTLRLAD
                     LASSAQDLPKLSAGRGTLEFSRGQTRLMLDGGEVEQLAVMSARVDWPRTGAPRMHAAL
                     QGDLASPALR"
BASE COUNT          359 a          733 c          751 g          380 t
ORIGIN      
        1 ggccgtggct tcgtgcgcac gcctgaaacg gtgtgccacg agatattccg tgaaattgtg
       61 cggcagagca ggcagttcca gagccgcgag ctgctaattt tggctcacca gaatgtgatc
      121 gatcgcctgc tggacgacga atcggcggtg ctggccgagc tcgagcacca ggtggggcgg
      181 ccgatacgat tgcaggtcga ggcgctgtat ggcttcgacc aatacgacgt ggtgctggcc
      241 taggagatga aacggagaga tgaccgacgc cgcccttgat ccaaggggag acgaaagcgg
      301 ctcgcactgg gcgagacgta cggtgctggt cacgttttgt ttattgctgc tcggcacact
      361 gggcgtggtg ttcacgcgcg tcgtcgcggc acgcgtgccg gagcaacgcg ccactctcga
      421 gaaactcatc gccgatcgca ccgggctcgc cgtgcgtttc gacaacgttc atttcgcgtg
      481 gaatctcgac ggcacgagcg ccgtgttcga gcgcgtcgag ctgaccgatc ccgcgcgcgg
      541 ccgcgtgcgc gtcgtcgcgc cggagctgcg cgtcgagttc gatacctggg atttcctgcg
      601 ccatcaccag ttttcgctgg gccacgtcac gctcgcttcg ccggatatcg aaatcatcgg
      661 cgatgcactc gagacgccgg tcgccgatgt gcgcgccccg aaagggaagg cgaacgcgca
      721 gaaggcggcg ccggcccagg aggacgaagc cacgctcgtt cgccgcttcg ccgcgtgggc
      781 cgagctcatg cccaacggcc gaatcgaagt cgagggcgcg cgtgtgcatc tgcggcgccg
      841 cggcgatcgc gcggcgcgta acaccttcac gctgagccag gcggtattga tccgcggcgc
      901 atccaacttc aacgcattcg gcaccatgct gctctcgcag gatgtcggcc agtcgctgtt
      961 cctgtccgcg aagctcgaag gtctcggcac gccggccggc gtgaccggcg atcttcgttt
     1021 gatcgcgcgc cgcgtgttcc tcgacaaggt gcccctcatc gacgctgccg gccgcggcac
     1081 gctcgatgcg acgctgcacc tcgagaacgg actcgtgcat accgcgaagt ggcaggcgag
     1141 tgcgcgcgaa ctcgagttcg acgacgaacg tgcgcgcttc gatcacttca ccgtcagcgg
     1201 caagctcgaa cgcgacgcgg acgatttcct gctgcagttc gccgatctgc agatcacgcg
     1261 gggcgcgcgc ctggagcgtg cgccgcgcct ctcagcgcgt atcagcatcg agccgggcac
     1321 gacgcgcatc gcgcgcacca cgctgcgctc cgagcgcgtt cctttcatgg cggccgagtt
     1381 catcacgcgt ttgcttgcac cccaggccgc gcatcgcatc agtgaattcc ccgggggatg
     1441 ggttccgacg ggaggcgagc ttcggacagt tcgcttcgat tccgggccga aatccggcca
     1501 ggatttcgcg gcctggacct tcaatgccca gatcgccgga ggagacctcg aacgtgcccg
     1561 cgatcacgcg cgtgtcagtg gagtcgccgc gatgttgcgc ctcagcaaag gcgaagtggc
     1621 gttcgacttc gatcccgcga acgcggtcaa cgtgcgcgct ccgggcgagc ttccgccgcg
     1681 tgcgctgaat ctcggcggcc ggctggtcat gctcagcacg ggcgatgccg tcgcccgctt
     1741 cgaaaccttc tccgcgcgca gcgccgacag cagcctcgcg ctcgacggcc agtggaacga
     1801 aggccagccg ctcgccgtca ccgtgtcgaa catcgatcgt gcgatggtgc tcgacggctg
     1861 gaggctgttt gcgaacgacg cggccgcgcc cgggtggtta ggtgagatcg ggagcggtgt
     1921 cgtgaccgag ggcaaactga accttctgac gctgcgcgat gccgacggcc atcgcgccgt
     1981 gaactggcag cgttccagcg gcacgttgcg gcttgccgac cttgcgagtt cggcgcagga
     2041 cctgccgaaa ctgtccgcgg gtcgcggcac gctcgaattc tcgcgcggac aaaccaggct
     2101 gatgctcgac ggcggtgaag tcgagcaact cgccgtcatg tccgcacgcg tcgactggcc
     2161 gcgcaccggc gcgccgcgca tgcacgccgc cttgcagggc gatctggcgt cgccggcgct
     2221 gcg
//
LOCUS       DATDEU010000540         6294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1709053, whole genome shotgun sequence.
ACCESSION   DATDEU010000540 DATDEU010000000
VERSION     DATDEU010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709053"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VIV63_12920"
     CDS             <1..566
                     /locus_tag="VIV63_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY6125547.1"
                     /translation="RPIGATDAIPVNVRVISATHRDLQQLMMSGQFREDLYYRLNVVH
                     IEMPPLNRRREDIPLLVSHFLAQISNESGVRKIYAPEAVELLATADWPGNIRQLSNVV
                     RQNVALSQTPIIPVELVQQSLGGTQSKLPSFDEARDEFTRSYLSQILQITGGNVSQAA
                     RLAKRNRTDFYKLLSRHQLTPEDFKGR"
     gene            600..1334
                     /locus_tag="VIV63_12925"
     CDS             600..1334
                     /locus_tag="VIV63_12925"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEY6125548.1"
                     /translation="MSFIGNLLLPSHLALLLLVTGLLARSSRRSQPWSGKLLVAGALV
                     ALVFSLGVVARLLLNPLEQRYPAWSPGVSPALTIDHIVLLTAWAGDNPDLPAGVRLND
                     SSASRALVTMQLWRLHPQTTVIVSGDERNARDLGEVLLTLGLPADRLRLETQSRNTGD
                     SALHVAPLLDGKPFALVTSAGHLPRSMATFAKAGLDALPVPADFRLPGPVSAASFLPA
                     PRALQASDLAVHEYLGLTWYRMLGRL"
     gene            1459..2655
                     /locus_tag="VIV63_12930"
     CDS             1459..2655
                     /locus_tag="VIV63_12930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6125549.1"
                     /translation="MKKKILYIEGNTDGTVGGSYYLMFDLVSRLDREKFEPIVGFHKD
                     NFLIERFRSIGVETIVFSNPVPWVPKGFLFKTLLSPLKKLVNFYRGFFVPAREYAQFL
                     RDRKIDLVNLNNSITRNHAWMLAARKTNTRCMTHEMGIRLTFSPVSRFLGKRLEKIIC
                     LSHAIHDNMKRLGVDYPNTVVIHCGIDLERYHVKETPEQLRAKHNIPADSEVVGVVGN
                     IREWKGQVVMVRAAALLKQKFPRLRVLLVGACGEADKAYGDMLAKLARELGIEDSVIF
                     TGFQSNAIDYMGLMDVVAHTSVHPEPFGIVTLEAMSLSKPLVSTTHGGPAEVVVNGET
                     GLLVEAGKPELLAAAIGSLLADRAKATEFGRKGYERLNQHFGIRKNIDATMAVYRSVL
                     RLPDAA"
     gene            2693..3658
                     /locus_tag="VIV63_12935"
     CDS             2693..3658
                     /locus_tag="VIV63_12935"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY6125550.1"
                     /translation="MSNFKRKRVLVSGGAGFLGSHLCDRLIAAGNDVLCIDNFFTGTK
                     DNIAHLVDNPHFELVRHDVTFPLYVEVDEIYNLACPASPIHYQHDPVQTTKTSVHGAI
                     NMLGLAKRVRAKIFQASTSEVYGDPAVHPQVEEYWGNVNPIGTRSCYDEGKRCAETLF
                     FDYHRQHKLRIKVARIFNTYGPRMHPADGRVVSNFIMQALQGDDITIYGDGAQTRSFC
                     YVDDLINAFLALMATGDDITGPINTGNPGEFTMLELAQQVIEITNSKSKIIHQPLPQD
                     DPKQRRPDITKAKKHLNWEPKVQLKEGLTRTVAYFDELIRKSKTA"
     gene            complement(3659..5530)
                     /locus_tag="VIV63_12940"
     CDS             complement(3659..5530)
                     /locus_tag="VIV63_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY6125551.1"
                     /translation="MRTWAILVVVLAALAGAGFWVLRHRWMVVNSLPVSWRDAIFGMR
                     YGYRVERDVMIAMPDGVKLATNLYFPKDVDLKQAMGKLPVVLIRLPYDKNHHAGALAP
                     AQDFAGHGYVVAIQDMRGKYASEGVFTPSRLDAEDGSATVDWLASQPWSNGRVGTVGC
                     SSLGESQIMLARMRNPHHAAMIPMGAGGAIGSAANRYTYFGQYEGGVFQLASGFGWFL
                     FEGGKTPGVEFDEKIDVARAIRDLPSGGLVRRHRPDKTDFDDFISKPLGDPYWRDLGY
                     IADEDRFATPALVFNTWQDQTVAETLVLADVMKRNAATDAARTRYHVIIGPGNHCQYF
                     GPNQTLNVGEFRAGPNALAPFSAWQRQWFDYWLKQGGAGKPPDFPAYRFYVMGADRWI
                     DSAQWPPAGVISQNWYLGGERAANSRAGSGALQLSAPTAEQADEFRYDPMNPVPTRGG
                     PICCTNDPAQVQGMVDQTEVESREDVLVYTSTVLESDLTIAGPLRADLYVSSSAKDTD
                     LIARLVDVFPDGRALNIQEGALRLRYRDSYVTPELMTPGEVYRVSVDMRAIAYELPKG
                     HRLRLQITSSNFPRLERNLNTGGNNFDESQPVTATNRILRSPQYPSALVLSVLPKTD"
     gene            complement(5541..>6294)
                     /locus_tag="VIV63_12945"
     CDS             complement(5541..>6294)
                     /locus_tag="VIV63_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04325 family methyltransferase"
                     /protein_id="HEY6125552.1"
                     /translation="QFDKQFATHVEGGFGGNLYRGVFKTFEEAQASAPAGKPLGYDNP
                     AAADLYLERTRRIYPSDYPVVYWLDRLFRSGLRTVFDFGGHIGLGYYAYRKYVNYPDG
                     LRWTVHDVPAVMARGRELAQTMDPEKRLSFAESVAGADGYDVFFSAGALQYLPTTLAE
                     MLAPLAKKPRALVLNLAPLHPAESFYTLQSIGAAFCPYRVLQFGAFVKSLTQLGYVQR
                     DAWENPDKRCTVAFEPGHSIDRYYGFTFELQA"
BASE COUNT         1157 a         2076 c         1932 g         1129 t
ORIGIN      
        1 tccgcccgat cggcgccacc gacgccattc cggtcaacgt gcgcgtgatc tccgcgaccc
       61 atcgcgacct gcagcagctc atgatgagcg gccagttccg cgaagacctc tattaccgcc
      121 tgaacgtcgt gcacatcgag atgccgccgc tcaaccgtcg ccgcgaagac atcccgctgc
      181 tggtctcgca cttcctcgcg cagatcagca acgaaagcgg cgtgcgcaag atctacgcgc
      241 ccgaagccgt ggagttgtta gccacggccg actggccggg caatattcgc cagttgtcca
      301 atgtggtccg ccagaacgtg gcgctgagcc agacgccgat catccccgtg gaactggtgc
      361 agcagtcgct gggtggcacg cagagcaagc ttccgtcgtt cgacgaggct cgcgacgagt
      421 tcacgcgcag ctacctgtcg cagatcctgc agatcaccgg cggcaacgtg agccaggccg
      481 cgcgcctcgc caagcgcaac cgcacggatt tctacaagct cctctcgcgc caccagctga
      541 cgccagaaga cttcaaaggc cgctaagatc gcgcgggtgt cgtcgacccg cgtgaatcca
      601 tgagctttat cggcaacttg ttgttgcctt cacatcttgc gcttctattg ctggtcaccg
      661 gactgctcgc gcggtcctcc cgccgctcgc agccctggtc gggcaaactg ctcgtggcgg
      721 gcgctctggt cgcgctggtg ttctccctgg gcgtcgtcgc ccgcctgttg ctgaacccgc
      781 tcgaacagcg atacccggcg tggtctcccg gcgtgagccc cgcgctgacc atcgaccaca
      841 tcgtgctgct caccgcgtgg gcgggcgaca accccgatct tccggcgggt gttcgtctca
      901 acgattcttc ggcgtcgcgc gccctggtca ccatgcaact gtggcggctg catccccaga
      961 ccacggtcat cgtgagtggc gacgaacgta acgcccgcga cctcggcgaa gttctgctga
     1021 cgctcggcct gcccgccgac cgccttcggc tcgaaacgca atcgcgcaat accggtgaca
     1081 gcgccctgca cgtcgcacct ttgttagacg ggaaaccctt cgcgctggtg acttcggccg
     1141 gtcatctccc gaggtcgatg gccacgttcg ccaaggcggg gctcgacgcc ctgcccgtac
     1201 ccgccgactt tcgtctgccc ggacccgtgt cggccgccag ctttctgccc gctcccaggg
     1261 cgcttcaggc ctcggacctt gcggtgcacg aatacctggg actgacgtgg taccgcatgc
     1321 tgggccggtt atgaagggtt ccgcccccgg aagtgaccca ggtcgcgttc ctgccatccg
     1381 ggttgaaggg gtctcggcag accctgcctc acgcgctgat aatgccggcc gcacagtggg
     1441 ttacattcag gcaggcggat gaagaagaag attctgtaca tcgaaggcaa cacggacggc
     1501 acggtcggtg gttcttacta tctgatgttc gacctggtct cgcgactcga tcgcgaaaaa
     1561 ttcgagccga tcgtcggctt ccacaaggac aacttcctga tcgaacgctt caggtccatc
     1621 ggcgtggaaa ccatcgtgtt ctccaatccc gttccctggg taccgaaagg gttcctcttc
     1681 aagacccttt tgtcgcccct caagaagctg gtgaatttct atcgcgggtt tttcgtgccc
     1741 gcgcgcgaat acgcgcaatt cctgcgcgac cgcaagatcg acctggtcaa tctcaacaat
     1801 tccatcacgc gcaatcacgc ctggatgctg gcggcgcgca agaccaacac gcgttgcatg
     1861 acccacgaga tgggcattcg cctcacgttc tcgccggtgt cgcgattcct cggcaagcgg
     1921 ctcgagaaaa tcatctgcct gtcgcacgcc atacacgaca acatgaagcg gctgggcgtc
     1981 gactatccga acaccgtcgt gatccattgc ggcatcgacc tcgagcgtta ccacgtcaag
     2041 gaaacacccg agcagctccg cgccaagcac aacattccgg ccgattcgga agtggtcggc
     2101 gtcgtgggca acatccgcga atggaagggc caggtcgtca tggtgcgcgc cgcggcactg
     2161 ctcaagcaga aattcccgcg cctgcgcgtg ttgctggtcg gcgcctgcgg cgaagcggac
     2221 aaggcctacg gcgacatgct ggcgaaactc gcgcgcgaac tcggcatcga agactccgtc
     2281 atcttcaccg gcttccagag caacgccatc gactacatgg gcctcatgga cgtggtcgcg
     2341 catacttcgg tccacccgga gcctttcgga atcgtcacgc tggaagccat gtcgctgtcg
     2401 aagccgctgg tgtccacgac ccacggcgga ccggccgaag tggtggtcaa cggcgagacc
     2461 ggcctcctgg tcgaagccgg caaacccgaa ctgctggccg ccgccatcgg atctttgtta
     2521 gccgaccgtg cgaaggcgac cgaattcggc cgcaagggct acgagcggct caatcagcat
     2581 ttcggcattc gcaagaacat cgacgcgacc atggcggtct accgcagcgt tttacgtttg
     2641 cccgacgccg cctgaatcca ccccatttcc tcgcaaggca gaaacttttc ccatgagcaa
     2701 tttcaagcgt aaacgtgtcc tcgtcagcgg cggcgcgggt ttcctgggtt cacatctgtg
     2761 cgaccggctg atcgccgccg gcaacgacgt gttgtgcatc gacaatttct tcaccggcac
     2821 caaggacaac atcgcgcacc tggtggacaa cccgcacttc gaactggtgc gccatgacgt
     2881 cacctttccg ctgtacgtcg aagtcgacga gatctacaac ctcgcctgcc cggcttcgcc
     2941 catccactac cagcacgacc cggtgcagac caccaagacc agcgtgcacg gcgccatcaa
     3001 catgctgggc ctcgccaaac gcgtgcgcgc caagattttc caggcgtcca cgtccgaggt
     3061 gtacggcgat cccgcggttc atccgcaggt cgaggaatac tggggcaacg tgaatcccat
     3121 cggtacgcgt tcgtgctacg acgaaggcaa gcgctgcgcc gaaacgctgt tcttcgatta
     3181 ccaccgtcag cacaagctgc gcatcaaggt ggcgcgcatc ttcaatacct acggcccgcg
     3241 catgcatccg gccgacggcc gcgtggtgtc caacttcatc atgcaggcgc tgcagggcga
     3301 cgacatcacg atctacggcg acggcgcgca gacgcgttcg ttctgctacg tcgacgacct
     3361 catcaatgcg ttcctggcgt tgatggcgac cggtgacgac atcaccggtc cgatcaacac
     3421 cggcaatccc ggcgaattca ccatgctcga gctggcgcag caggtcatcg agatcaccaa
     3481 ctcgaagtcg aagatcattc accagccgct gccgcaggac gatccgaagc agcgccgccc
     3541 ggacatcacc aaggcgaaga agcacctgaa ctgggaaccg aaggtgcagc tcaaggaagg
     3601 cctgacgcgc accgtcgcgt acttcgacga gctgatccgc aagtcgaaga ctgcgtagtt
     3661 agtcggtctt cggcagcacg gacagcacca gcgccgatgg atattgcggc gatcgcaata
     3721 tccggttggt ggcggtcacc ggctggcttt cgtcgaagtt gttgccgccg gtgttgagat
     3781 tgcgctcgag ccgcgggaag ttgctgctcg tgatctgcag ccgcaggcga tgccccttgg
     3841 gcagctcgta ggcaatggcc cgcatatcca ccgacacgcg gtacacctct cccggcgtca
     3901 tgagctcggg cgtgacgtag ctgtcgcgat accgcaggcg caacgcgcct tcctggatgt
     3961 tgagtgcgcg accgtcggga aacacgtcca cgagcctggc gatgaggtcg gtgtccttcg
     4021 cgctcgagct cacgtagaga tcggcacgca ggggacctgc gatcgtgaga tccgactcga
     4081 gcaccgtcga ggtgtacacg agcacgtctt cgcgcgactc gacctcggtc tgatcgacca
     4141 tgccctgaac ctgcgccgga tcattggtgc agcagatggg gccgccgcgc gtgggcaccg
     4201 gattcatcgg gtcatagcgg aattcatcgg cctgctccgc ggtgggcgcg gagagttgca
     4261 aggcgccgct gcccgcacgg ctgttggccg cacgctcgcc gccgagatac cagttctgcg
     4321 aaatcacgcc ggcgggtggc cattgcgcac tgtctatcca ccggtccgcg cccatcacgt
     4381 agaagcgata ggcggggaaa tcgggcggtt tgcctgctcc gccctgcttc agccagtagt
     4441 cgaaccactg ccgttgccag gcgctgaacg gcgccagcgc gttgggaccg gcgcggaact
     4501 cgccgacgtt cagtgtctga ttcggcccga aatactggca gtgatttccg ggcccgataa
     4561 tcacgtggta gcgagtccgc gcggcgtcgg tcgcggcgtt gcgtttcatg acgtcggcca
     4621 gcaccagcgt ttcggccacg gtctggtcct gccaggtgtt gaacaccagc gccggcgtcg
     4681 cgaagcggtc ttcgtcggcg atgtaaccca ggtcgcgcca gtacggatcg cccaggggct
     4741 tcgagatgaa atcgtcgaag tcggtcttgt caggccggtg ccgccgcacc agcccgcccg
     4801 agggcaggtc ccggatggcg cgcgccacgt ctatcttctc gtcgaactcg acgccgggcg
     4861 tcttgccgcc ttcgaacagg aaccagccga atccggacgc gagctggaac acgccgcctt
     4921 cgtactgtcc gaagtaggta tagcgattgg cggcgctgcc gatcgcgcca ccggcgccca
     4981 tcgggatcat ggccgcgtga tgcgggttgc gcatgcgcgc cagcatgatc tgcgactcgc
     5041 cgagcgaaga gcagcccacc gtgcccacgc ggccgttgga ccagggctgc gacgcgagcc
     5101 agtcgacggt ggcggagccg tcttccgcat cgagccggct cggtgtgaag acgccttccg
     5161 aagcgtattt gccgcgcatg tcctggatgg cgaccacgta gccgtggccg gcgaagtcct
     5221 gggcaggcgc cagtgcgccg gcgtgatggt tcttgtcgta cggcaaccgg atcaggacca
     5281 cgggtagttt gcccatcgcc tgcttcaggt ccacgtcctt cggaaaatac aggttggtag
     5341 cgagcttcac gccgtcgggc atggcgatca tcacatcgcg ttccacgcga tagccgtaac
     5401 gcatgccgaa gatggcgtcg cgccagctca caggcagcga attcaccacc atccagcggt
     5461 ggcgcaagac ccagaagccg gcgcccgcca acgcggcaag caccaccacc agaattgccc
     5521 acgttcgcac gcccaaggtt ttacgcctgc aattcgaagg tgaaaccgta gtagcggtcg
     5581 atggagtgtc cgggttcgaa ggcgacggtg cagcgcttgt cgggattttc ccaggcgtcg
     5641 cgttgaacgt agccgagttg ggtgaggctc ttcacgaacg ccccgaattg aagcacgcga
     5701 taggggcaga acgccgcgcc gatgctctgc agcgtgtaga acgattcggc agggtgcagc
     5761 ggcgcgaggt tgagcacgag cgcgcgcggc ttcttggcga gcggcgccag catctcggcc
     5821 agggtagttg gcaggtactg cagggcgccg gccgaaaaga acacgtcata gccgtccgcc
     5881 cccgccacgc tctcggcgaa tgacaatcgt ttctcgggat ccatggtctg ggcgagctcg
     5941 cggccgcggg ccatgacggc gggcacgtcg tgcacggtcc agcgcaggcc gtcgggataa
     6001 ttcacgtatt tccggtacgc gtaatagccg agaccgatgt gcccaccaaa atcgaacacc
     6061 gtccgcaacc cgctgcggaa cagacggtcc agccagtaaa ccaccgggta gtcgctgggg
     6121 tagatgcggc gcgtgcgctc caggtacagg tcggccgcgg ccgggttgtc gtaacccaga
     6181 ggtttgccgg caggggcgct cgcctgggcc tcctcgaagg tcttgaaaac cccccggtac
     6241 agattgccgc cgaagccgcc ctccacgtgg gtggcgaact gcttgtcgaa ctgg
//
LOCUS       DATDEU010000541         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2146743, whole genome shotgun sequence.
ACCESSION   DATDEU010000541 DATDEU010000000
VERSION     DATDEU010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146743"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VIV63_12950"
     CDS             complement(<1..683)
                     /locus_tag="VIV63_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125553.1"
                     /translation="MKQSLSFALEPRARASIDDYVADIVWAPDSRSFAIAGGEGKVAL
                     ARVEADAIAIEVIGEHLLGTLSLVWRPRANVFATSGQDGAVALWDGESGKEIKRWKPA
                     PTPTQALSYSPDGEILASAAGKVVTLWSSPGDKIHAFAPAASTAVSLSFDRPGTDLGV
                     ALNGELVVHRIEKSRYETRRYRWPAACLTVNFSGNGRFLASGMADGTLHFWNRSTGKD
                     SQMRGYDGKL"
     gene            complement(692..2110)
                     /locus_tag="VIV63_12955"
     CDS             complement(692..2110)
                     /locus_tag="VIV63_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6125554.1"
                     /translation="MSVTPSRVPVTVLTGFLGSGKTTLLNRILSEKHGKRIAVIENEF
                     GEVGVDHQLVIGAEEELFETNNGCICCTVRGDLIRILGQLMKRRDKFDYILIETTGMA
                     DPGPVAQTFFLDDELKNQFSLDAIVTMVDAKHFEQHVDELNEPAEQVAFADLVLLNKT
                     DLVDEASLERIERRIRGINGTAKILRTKNADAPIDRVLNVGAFDLQRALTVDATFLEP
                     EYPFEWGGVYQLTPGDYLLKTAGEHDHGAHAHGHDHGHDHDHDHEDGHDHGHTHEDLD
                     IVVLPLTAATPAALDAAIETAVRAFADAATVIPCHGTLDAAPSHNAIDISHPGADFKL
                     TIDKAGAYALFCEHHPLEFGLHLVGQRPAAERKFASHHHDDDISSIGISDPRPLDPVK
                     VNQWFDYLLKTRGQDIFRMKGVLNVKGEDRRQVFHGVHMMFDAQAERPWGDAERVNSL
                     VFIGRDLDRAEFEAGFESAVAK"
     gene            complement(2139..>2405)
                     /locus_tag="VIV63_12960"
     CDS             complement(2139..>2405)
                     /locus_tag="VIV63_12960"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY6125555.1"
                     /translation="DAWQSQWMPELMASLPISGVDGTMKARNVPTGAAHMKTGLLGDT
                     RAVAGYVLAASGRRYVVVAIINHPNASRGTSAHDALIDWVYRVG"
BASE COUNT          396 a          764 c          775 g          470 t
ORIGIN      
        1 agcttgccgt catagccgcg catctgtgaa tccttgcccg tcgagcgatt ccagaaatgc
       61 agcgtgccgt cggccatgcc acttgcgaga aatcttccat tgccgctgaa gtttaccgtg
      121 agacacgcgg ccggccacct gtagcgtcgc gtttcgtagc gcgatttctc gatacggtgc
      181 acgacgagct cgccattcag ggcaaccccg agatcggttc cgggtctgtc gaaggacaag
      241 ctgaccgcgg tgctcgcggc cggtgcgaac gcgtgaatct tgtcgcccgg cgaggaccac
      301 aacgtcacga ccttgccggc ggcgctcgcg agaatttcgc cgtcgggcga ataactcaag
      361 gcctgcgtcg gagtgggcgc cggtttccag cgcttgatct ccttgccgga ttcaccatcc
      421 cacaacgcga ccgcgccgtc ctggccggag gtcgcgaaca cgtttgcacg cgggcgccag
      481 acgaggctca gcgtgcctaa caggtgttca ccgatcactt cgatcgcgat cgcgtcggct
      541 tcgacgcgag ccagcgccac cttgccttcg ccaccggcga tcgcgaacga ccggctgtca
      601 ggcgcccaca cgatgtccgc aacgtagtcg tcgatgctgg cgcgcgcgcg cggctccagc
      661 gcgaatgaca gggactgctt cacggccatg attatttcgc gacggcggat tcgaagccgg
      721 cttcgaactc ggcgcgatcg aggtcgcggc cgatgaagac gagactattc acacgttcgg
      781 cgtcgcccca gggcctttcc gcctgcgcgt cgaacatcat gtgcacgccg tgaaatacct
      841 ggcgccggtc ttcgcccttc acgttcagca cacccttcat gcggaagatg tcctgcccgc
      901 gcgtcttcag cagatagtcg aaccactggt tgaccttcac gggatcgagc ggacgcggat
      961 cgctgatgcc gatggagctg atgtcatcgt catggtgatg cgatgcgaac ttgcgttcgg
     1021 ccgcgggccg ctggccgacc aagtgcaatc cgaactccag cggatggtgt tcgcagaaca
     1081 gcgcgtacgc gccggccttg tcgatggtga gtttgaaatc cgcgcccggg tgcgagatgt
     1141 cgatggcgtt gtgcgaaggc gcggcatcca gcgttccgtg gcacggaatg accgtggcgg
     1201 cgtccgcaaa cgcgcgcacc gccgtctcga tcgcggcgtc gagcgccgcg ggcgtggccg
     1261 ccgtcagcgg cagcaccacg atgtcgagat cttcgtgcgt gtggccgtga tcgtgcccgt
     1321 cttcatgatc gtggtcgtgg tcatgcccgt ggtcgtgccc atgcgcgtgc gcgccgtggt
     1381 catgctcgcc ggcggtcttc agtagatagt caccgggcgt gagctgataa acgccgcccc
     1441 actcgaacgg gtattcgggt tccaggaacg tggcgtccac ggtcaacgcg cgttgcagat
     1501 cgaacgcgcc gacgttcagc accctgtcga tgggcgcatc ggcattcttc gtgcgaagga
     1561 tcttcgccgt gccgttgatg ccgcggatgc gtcgctcgat gcgttcgagc gacgcctcgt
     1621 cgaccaggtc cgtcttgttg agcagcacca ggtcggcgaa ggccacctgc tccgccggct
     1681 cgttgagctc atcgacgtgc tgctcgaagt gcttcgcgtc caccatcgtc acgatggcgt
     1741 ccagcgagaa ctggttcttg agctcgtcgt cgaggaagaa ggtctgcgcc accgggcccg
     1801 gatcggccat gcccgtggtt tcgatgagaa tgtagtcgaa cttgtcgcgc cgcttcatga
     1861 gctggccgag gatgcggatg aggtcgccac gcaccgtgca gcagatgcag ccgttgttgg
     1921 tctcgaacag ctcttcttcc gcgccgatga cgagctggtg gtcgacaccg acctcgccga
     1981 attcgttctc gatgaccgcg atgcgcttgc cgtgtttctc gctcaggatg cgattcagga
     2041 gcgtggtttt tccggagcca aggaagcccg tgagtaccgt gaccggcaca cgtgatggag
     2101 tgactgacat agggcgcgga ttataggggc aggacccctc atccgaccct gtaaacccag
     2161 tcgattagtg cgtcgtgggc ggaagttccg cggcttgcgt tcgggtggtt gatgatggcc
     2221 accaccacgt agcgccggcc gctggccgcc agtacataac cggccacggc gcgtgtatcc
     2281 cctagcaggc cggtcttcat gtgggccgcg ccggtgggta cgttgcgcgc cttcatggtg
     2341 ccgtccacgc ccgagatcgg caaagaggcc atcagctccg gcatccactg gctctgccag
     2401 gcgtc
//
LOCUS       DATDEU010000542         5579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_249681, whole genome shotgun sequence.
ACCESSION   DATDEU010000542 DATDEU010000000
VERSION     DATDEU010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249681"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..884
                     /locus_tag="VIV63_12965"
     CDS             129..884
                     /locus_tag="VIV63_12965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6125556.1"
                     /translation="MVFALYIVSGLLSLGLIVHCVKTQRNTIWIYVLVMLITFPFVGS
                     LVYIGAEILPELLRSRTSQRAMRGIRTTLDPEGNLRKFENEMNVAGNVAARQRYADEL
                     VRLGRAEEALPIYQTCLTGVFTDDPKLLLGFAHAQFEAGQPAAARKTLDDLIGKNPDF
                     KSADGHLLYARAVEAEGDLAKALSEYATLAEYYPGAEAGVRYARLLKKSDQAPLAQQT
                     LKALLDRAKYAPAHYRKAQRVWLDEAHRELQNT"
     gene            complement(908..1099)
                     /locus_tag="VIV63_12970"
     CDS             complement(908..1099)
                     /locus_tag="VIV63_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125557.1"
                     /translation="MAKKKGARHMREWSAGDMQKLRQFAKQKVSARVAAQKLGRSPGA
                     VRYKAMMEGVSFRSINRKK"
     gene            complement(1229..2059)
                     /locus_tag="VIV63_12975"
     CDS             complement(1229..2059)
                     /locus_tag="VIV63_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEY6125558.1"
                     /translation="MSQRPVPRRDIPDRLRIRPASRTKLLDAEADRCFGWDKDKALAA
                     TAENLARLEELQYKMYADQRFAMLIALQAIDGGGKDSTIRRVFGAFNPQGCTVTAFKA
                     PSAEDLRHDYLWRIHRHAPPRGEIAVFNRSHYEDVLIVRVNDLVPEDVWSRRYEQINQ
                     FEGLLSACGTRVVKIFLQISKEEQRVRFQERLTDRRKNWKFDTGDLEKRQQWAAYTRA
                     FEAMFARCSTDHAPWYVVPANRKWFRDFAVSQLLIYEFEQLPLRFPAPNFDPKTIKLV
                     "
     gene            complement(2056..3147)
                     /gene="aguA"
                     /locus_tag="VIV63_12980"
     CDS             complement(2056..3147)
                     /gene="aguA"
                     /locus_tag="VIV63_12980"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF010070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEY6125559.1"
                     /translation="MAKTLESTPLADGFSMPAEYSPHAGCWMLWPERPDNWRLGALPA
                     QQAFANVATAIARFEPVTVGASAGHYEFARAQLPAHVRVVEMSHDDAWMRDVGPTFVT
                     NERGERRGVDWRFNAWGGLSGGLYFPWDQDDLVANKVLEIEGNDRYRAHIVNEGGAIH
                     VDGEGTALVTEQVLLNANRNPGMTREEMERHLKAYLGVQVVVWLGEGVVDDETGGHID
                     NLACFLRPGTVALHWTDNKRDPQFAVSQNALERLSAVRDARGRRIAVIKLPAPGPLRY
                     RPDEVVGVQVRDTVRGRIAGMRLAGSYVNFYIANGGIVMPLLDSRTDKAAAAKLKRAF
                     PGRKVVGVPAREILLGGGNIHCITQQVPR"
     gene            3250..4563
                     /gene="icd"
                     /locus_tag="VIV63_12985"
     CDS             3250..4563
                     /gene="icd"
                     /locus_tag="VIV63_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY6125560.1"
                     /translation="MATTPTSPKVTPPAGGAKITIQNGKLAVPDQPIIPFIIGDGTGP
                     DIWHASVRVMDAAVAKAYGGKKKIHWLEIYAGEKSFKAFNNWLPDETVEACREYLVSI
                     KGPLTTPIGGGIRSLNVALRQMLDLYVCLRPVRWFKGVPSPVKAPDKVDMVIFRENTE
                     DIYAGIEFEHGSEENKKFKELFKQAFPKAFAKIRFPDTSGIGFKPVSKEGTERLFKSA
                     LEYAIAFKRRSVTIVHKGNIQKFTEGAFRNWAYALAESGEFANKFYTWEQWGRTKAAS
                     GEKAANDEQAAALKSGKILVKDAIADIALQQVLTRPDEFDVIATTNLNGDYLSDALAA
                     QVGGIGIAPGANINYLTGHAVFEATHGTAPKYADQDKVNPGSVILSGEMMLRYMGWTE
                     AADAIISAMDKTIAQKTVTYDFARLMDGAKEVKCSEFGDALIRNM"
     gene            complement(4630..>5579)
                     /locus_tag="VIV63_12990"
     CDS             complement(4630..>5579)
                     /locus_tag="VIV63_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6125561.1"
                     /translation="QGRDDCLATASGVNAIAQTLIGLTKQGDHILCFVETYGPTRNII
                     RRLLSRFGVEYTMLSIEDMAGIENVLATRPTRVVIFESPTNPITKVADIERLVELAHR
                     HGALAVMDNTFAGFHQHGEYEVDLFVHSLTKYASGTGDVMGGAVIGRRELIEGLRPDF
                     AVLGALLDPHAAFLVQRGLKTYFIRYRDQTLSAQAIAEHLAAHPAVSKVHYPGLPGHP
                     HAALAKKQMREAGTVVSFDLKEGGEAARRFCESLKLFALTASLGSTESLVMAPQLIGT
                     RDFTAEQARQSGIAAGTVRLSIGLEDLGDLIADIDQALG"
BASE COUNT         1056 a         1795 c         1727 g         1001 t
ORIGIN      
        1 acaatgtttt cgacaagcgt tacgcggatc gcgcagactt cgctttcggc aactaccgct
       61 atttcccggc gcgcggccgc gcggtgttcc tgtccttgga ttacgcgcgc aactagagag
      121 gctcgaagat ggtgtttgcg ctctacatcg tttccggatt gctctcgctc gggctcatcg
      181 tccactgcgt caagacgcag cggaacacga tctggatcta cgtgctggtc atgctcatca
      241 cgttcccgtt cgtcggctcg ctggtctaca tcggcgctga aatcctgccc gagctgctgc
      301 gctcgcgcac cagtcaacgc gcgatgcgcg gcatccgcac cacgctcgat ccggaaggca
      361 accttcgcaa gttcgagaac gagatgaacg tggccggcaa cgtcgcggcc cggcagagat
      421 atgccgacga actcgtgcgg ctcggcaggg cagaagaagc cctgcctatc tatcagactt
      481 gtctgacggg tgttttcacc gacgatccca agctgctgct cgggttcgcg cacgcgcagt
      541 tcgaggcggg gcagcccgcc gcggcgcgca agacgctcga tgacctcatc gggaagaatc
      601 cggacttcaa gtccgcggac gggcacctgc tgtacgcccg cgcggtggag gccgaagggg
      661 acctggccaa ggctttatcg gagtacgcga cgcttgcgga gtactacccg ggcgccgagg
      721 ccggggtgcg ttacgcgcgc ttgttgaaga agtcggatca ggcgccgctg gcccagcaga
      781 cgctcaaggc gctgctggac cgggccaaat atgctccagc tcactatcgc aaggcccagc
      841 gcgtgtggct ggacgaagcg caccgcgagc tgcaaaacac ctaggaaatg ctaggtttgc
      901 agctccgtta tttcttgcgg ttgatcgacc ggaaggacac gccttccatc atggccttgt
      961 aacgcaccgc accgggcgag cgaccgagct tctgtgcggc gacgcgcgcc gacactttct
     1021 gcttggcgaa ctggcgcagc ttctgcatat cgcccgcaga ccattcgcgc atgtgccgag
     1081 cacctttctt cttggccatt ccggagtcct ctcgatacgt ggtgggaatt tggaatggcg
     1141 tagtctaaca acaattcccc acccggtagt caatcgtcgc ctaatgcaga catgtcggaa
     1201 ttccggcgcc tatcaacctc gcccggcgtc aaacaagctt gatcgttttt ggatcgaagt
     1261 taggggcggg aaaacgcagc ggcaactgct cgaactcgta aatcagcagc tgtgagaccg
     1321 cgaaatcgcg gaaccatttg cgatttgcgg gcactacgta ccagggcgcg tgatcggtgg
     1381 agcaccgggc aaacatcgcc tcgaatgccc gcgtgtaggc ggcccactgc tgtcgcttct
     1441 ccagatcgcc ggtatcgaac ttccagttct tgcgccggtc cgtgagccgc tcctggaatc
     1501 gcactcgctg ctcttccttg ctgatctgca ggaaaatttt tacaacgcgg gtgccgcagg
     1561 cactgagcag gccctcgaat tgattgatct gttcataacg cctgctccaa acatcttcgg
     1621 gaaccaggtc gttgacgcgc acgatcagca cgtcctcgta gtgcgagcga ttgaacactg
     1681 cgatctcgcc ccgcggcggt gcatgccggt ggatgcgcca caaatagtcg tggcgcagat
     1741 cttcggcact cggcgccttg aaggccgtca cggtgcaacc ctggggattg aacgcgccaa
     1801 acactcgccg gatcgtgctg tccttgccgc cgccgtcgat cgcctgcagc gctatcaaca
     1861 tcgcgaagcg ctgatcggcg tacatcttgt attgcagctc ttcgagccgc gccagattct
     1921 cggcggtcgc ggccagcgcc ttgtccttgt cccagccgaa gcaacggtct gcctccgcgt
     1981 ccagcaactt ggttcgcgaa gccgggcgga tacgcagccg gtcggggatg tcgcgtcgcg
     2041 gcacaggtct ctggctcatc ggggcacctg ctgagtaatg caatggatgt tgccgccgcc
     2101 taacaggatt tcccgcgcgg gcacaccgac gaccttgcgt cccggaaatg cacgcttgag
     2161 tttcgcggcc gcggctttgt cggttcgcga gtccagcaag ggcatgacga tgccgccatt
     2221 ggcgatgtag aaattcacgt agctcccggc gagacgcatt ccagcgatcc tgccacgcac
     2281 ggtgtcgcga acctgcacac cgacgacttc gtccggccgg tagcgcagag gtcccggagc
     2341 cggcagcttg atcacggcga tgcgacggcc gcgcgcgtcg cgcacggcgg agagtcgttc
     2401 gagcgcattc tgcgaaaccg cgaactgcgg atcgcgcttg ttgtcggtcc agtgcaatgc
     2461 caccgtgccc ggccgcagga agcacgcgag attgtcgatg tggccgccgg tttcgtcgtc
     2521 cacgacacct tcgccgagcc acacgacaac ctgcacgccg agatatgcct tgaggtgccg
     2581 ttccatctct tcccgcgtca tgccgggatt gcgattcgcg ttcagcagca cctgctcggt
     2641 gacgagcgcc gtcccctcgc cgtcgacgtg aatggcgccg ccttcgttga cgatgtgcgc
     2701 acggtagcga tcgttgccct cgatctcgag aaccttgttt gcaacgagat cgtcctggtc
     2761 ccagggaaag tagagcccgc ccgagagtcc gccccaggca ttgaagcgcc agtcgacgcc
     2821 acgacgctcg ccgcgctcgt tagtcacgaa cgtcgggccc acgtcccgca tccacgcatc
     2881 gtcgtgggac atctcgacca cgcgcacgtg cgcgggaagc tgcgcccggg cgaactcgta
     2941 gtgcccggcc gatgcaccga cggtgaccgg ttcgaatcgg gcgatggcgg tcgccacgtt
     3001 cgcgaaagcc tgctgcgcgg gcagtgcgcc cagtcgccag ttatcgggcc gttcgggcca
     3061 gagcatccag caccccgcgt ggggcgagta ttcggccggc atggagaaac catcggcgag
     3121 cggggtggat tcgagcgttt ttgccatggg tcgggattgt agcggtcctg agatgccatc
     3181 aagcacgtgt gctagcatcg ccgcccttcc gaccccccgc ccgcatcaaa gcgagagaaa
     3241 gtaaacacga tggccaccac gcccacgagt cctaaagtca cgccgccggc aggcggagcg
     3301 aagatcacca tccagaacgg caagctggcc gtcccggacc agccgatcat tccgttcatt
     3361 attggtgatg gcaccgggcc ggacatctgg cacgccagcg tgcgcgtcat ggatgccgcg
     3421 gtggccaagg cgtacggcgg caagaagaag attcactggc tcgaaatcta cgcgggcgag
     3481 aagtcgttca aggcgttcaa caactggctg cccgatgaaa ccgtcgaggc ctgccgcgaa
     3541 tacctggtgt ccatcaaggg cccgctgacc acgccgatcg gcggtggcat ccgctcgctg
     3601 aacgtggcgc tgcgacagat gttagacctc tacgtctgcc tgcggcccgt gcgctggttc
     3661 aagggcgtgc cctcacccgt caaggcgcct gacaaggtcg acatggtgat cttccgtgag
     3721 aacacggaag acatttacgc cggcatcgag ttcgaacacg gctccgaaga gaacaagaag
     3781 ttcaaggaac tgttcaagca ggcgttcccg aaagcgttcg cgaagatccg cttcccggac
     3841 acttccggca tcggcttcaa gcccgtgtcg aaggaaggta ccgagcgcct gttcaagtcg
     3901 gcactggaat acgcgatcgc gttcaagcgc cgcagcgtga ccatcgtgca caagggcaac
     3961 atccagaagt tcaccgaagg cgcgttccgc aactgggcgt atgcgctggc cgagagcggc
     4021 gagttcgcga acaagttcta cacctgggaa cagtggggcc gaaccaaggc cgcgtcgggt
     4081 gagaaggcgg ccaacgacga gcaggctgcc gccctgaaga gcggcaagat cctcgtgaag
     4141 gacgccattg ccgacatcgc gctgcagcag gttctgacgc gtccggacga gttcgacgtg
     4201 atcgccacga cgaacctgaa cggtgactat ctatcggacg cgctggcggc gcaggtgggc
     4261 ggcatcggca tcgcgccggg cgccaacatc aactacctca ccggccacgc cgtattcgaa
     4321 gccacgcacg gtacggcgcc caaatacgcc gaccaggaca aggtcaaccc gggctccgtg
     4381 atcctgtcag gcgagatgat gctgcgttac atgggctgga ccgaagcggc cgacgccatc
     4441 atctccgcga tggacaagac gatcgcccag aagacggtca cctacgactt cgcccgcctc
     4501 atggacggcg cgaaggaagt gaagtgctcg gaattcgggg acgcgctgat ccgcaatatg
     4561 taaatgggga cactccccat ttgctagcaa aaggggacgc acctgcgtcc ccttttcttt
     4621 ggggtcaaac taccccagcg cctggtcgat gtcggcgatg aggtcgccca ggtcctcgag
     4681 cccgatggac agccgcacgg tacccgccgc gatccccgac tgacgcgcct gctcggcggt
     4741 gaaatcgcgc gtaccgatga gttgcggggc catcaccagc gactcggtcg accccaggct
     4801 cgccgtcaac gcaaacaact tgaggctctc gcagaagcgg cgcgccgcct ccccgccctc
     4861 cttcagatcg aaactcacca ccgtacccgc ctcgcgcatc tgctttttcg cgagcgcggc
     4921 gtgcggatga ccgggcagac ccgggtagtg caccttgctc accgccggat gcgcggccag
     4981 atgttcggcg atcgcctgcg cgctcagcgt ctggtcccga tatcgaatga agtaggtctt
     5041 gagtccgcgc tgcaccagga acgcggcatg cggatccagc aaggcgccca gcaccgcgaa
     5101 atcaggccgc aatccctcga tgagttcgcg ccggccgatc accgcgccgc ccatgacatc
     5161 gcccgtgccc gaggcgtact tggtcaggct gtgcacgaac aggtcgactt cgtattcacc
     5221 gtgctggtga aagccggcga acgtgttgtc catgaccgcg agcgcgccgt gccgatgcgc
     5281 aagctccacc aggcgctcga tgtcggccac cttggtgatg ggattcgtgg ggctctcgaa
     5341 gatcacgacg cgcgtgggcc gcgtggccag cacgttttcg atgcccgcca tgtcctcgat
     5401 ggacaacatg gtgtactcga caccgaaccg cgacagcagc cgccggatga tgttgcgcgt
     5461 cggcccgtag gtctcgacga aacagaggat gtggtcgccc tgcttcgtga gaccgatgag
     5521 tgtctgcgcg atggcgttca cgcccgacgc ggtagcgagg cagtcgtcgc ggccctgga
//
LOCUS       DATDEU010000543         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_980374, whole genome shotgun sequence.
ACCESSION   DATDEU010000543 DATDEU010000000
VERSION     DATDEU010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980374"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..525
                     /locus_tag="VIV63_12995"
     CDS             58..525
                     /locus_tag="VIV63_12995"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY6125562.1"
                     /translation="MWYLALKSLHIVSVVLFLGNIITGVFWKVHADRSGDLRVREQAI
                     FGLIRSDRYFTVPGVFLIIATGVAMALLAHLPMLRTFWIGWSLALFGVSGLAFAFKVG
                     PLQKQMHANLTAGLAGSWNEAEYATLSRSWGIWGTVATAAPLVAVFLMVMKPV"
     gene            676..1938
                     /locus_tag="VIV63_13000"
     CDS             676..1938
                     /locus_tag="VIV63_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6125563.1"
                     /translation="MPRLHVPQPPTRPGDKPDFSYVELSPAGAIPMPEVNARARDMEG
                     VSVGLVRVLDDKHEAVGQWNPRLEAEDLQVGLRHMLLTRIFDDRMQRTQRQGKISFYM
                     RSFGEEAVAVAQAMALQPGDMLFPSYRQQGIYMVRGKPLVELMCQLLSNTRDMCKGRQ
                     LPVMYHWNAGRIFSISGNLTTQFPQAVGWAMAAAIRNEDHIACTWIGEGSSAEADFHH
                     GIIFAAVHQAPVIMNIVNNQWAISTFQGTAGGEQRSFAARGPGYGIAGIRVDGNDFLA
                     VYSVTQWAAQRARTGGGPTLIELVTYRASAHSTSDDPSRYRPKDDYEHWPLGDPVDRL
                     KNHLIARGEWSEKQHKELHAELDAQVVTAWKEAVSFGALNEGPRLDRHLMFEDVYKEF
                     PDNLRRQSEQLAEEIKLNAELGLVAKVD"
     gene            1941..>2897
                     /locus_tag="VIV63_13005"
     CDS             1941..>2897
                     /locus_tag="VIV63_13005"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY6125564.1"
                     /translation="MAQMNMIQALNSAMDVMMARDPSVVVMGEDVGYFGGVFRVTDGL
                     QRKYGEHRVLDSPISEGGIVATAIGMAVNGLKPVAEIQFADYIYPAVDQIASELARIR
                     YRSAGDFSSPVVIRAPCGGGIRGGQTHSQSPEAIFTHICGVKVVMPSNPYDAKGLLIA
                     AIEDDDPIIFFEPKRIYNGPFDGDPNKPAVPWSTHAKGNVPEGHYKVPIGKCDIVRPG
                     EQVTIVTYGTMVYVCEAAAKKLNIDAEIIDVRTMSPLDIDPICSSVKKTGRCVVAHEA
                     TRFSGFGAELSASIQEECFWYLEAPIQRVAGWDTPYPHAFEWE"
BASE COUNT          536 a          959 c          912 g          490 t
ORIGIN      
        1 agacttcgaa ttgaacgccg ccgggctcgt cgcctggctg aagcgcgagg ctaactaatg
       61 tggtacctgg ccctgaagtc gctgcacatc gtcagcgtgg tgctgttcct cggcaacatc
      121 atcacgggcg tgttctggaa agtgcacgcc gatcgcagtg gcgacctgcg cgtgcgcgag
      181 caggccatct tcgggctcat ccgcagcgac cggtacttca ccgtgcccgg cgtgttcctc
      241 atcatcgcca ccggtgtcgc catggcgttg ctggcgcacc tcccgatgct gcggacgttc
      301 tggataggct ggtcgctggc gttgtttggt gtttcgggcc tcgcattcgc gttcaaggtc
      361 gggccgttgc agaagcagat gcacgccaat ctcaccgccg gactggccgg tagctggaat
      421 gaggctgaat acgccacgct gtccaggtcc tggggaatct ggggaaccgt ggcgacggcg
      481 gcgccgctgg tagccgtgtt cctcatggtg atgaagccgg tgtaggcggg catgaagccg
      541 gaacgcctgc gcaggacctc gggcccgatc agtttcacct gaaactaatc accgccgatc
      601 gccggcgggc tgacgggctg tcaagtacaa cccgcgcgcc ttccctccct acccgcccgc
      661 gctatcatcc gcgccatgcc aagacttcac gtaccgcagc cgcccactcg accgggcgac
      721 aagccggatt tcagctacgt cgagctttca ccggccggcg ccatccccat gcccgaagtg
      781 aacgcccgcg cccgcgacat ggaaggcgtg tcggtggggc tcgtgcgcgt gctcgacgac
      841 aaacacgaag cggtgggcca gtggaatccg cgcctcgaag ccgaggacct gcaggtgggc
      901 ctgcgccaca tgctgctcac gcgcatcttc gacgaccgca tgcagcgcac gcagcggcag
      961 ggaaagatct ccttctatat gcgcagcttc ggcgaagaag ccgtggccgt ggcgcaggcc
     1021 atggcgctgc agcccggcga catgttgttc ccgtcgtacc ggcagcaggg catctacatg
     1081 gtccgcggca agccgctggt cgagctcatg tgtcagctgc tctcgaacac acgcgacatg
     1141 tgcaagggcc ggcagctccc ggtcatgtac cactggaacg caggccgcat cttctcgatc
     1201 tccggcaacc taactacgca gttcccgcag gccgtgggct gggccatggc cgcggcgatc
     1261 cgcaacgaag accacatcgc ctgcacctgg atcggcgaag gctcgagcgc cgaagccgac
     1321 ttccaccacg gcatcatctt cgcggccgtg caccaggcgc cggtcatcat gaacatcgtc
     1381 aacaaccagt gggccatctc cacgttccag ggaacggccg gcggcgagca gcgctcgttc
     1441 gcggcgcgcg gcccgggtta cggcatcgcc ggcatccgcg tcgacggcaa cgacttcctc
     1501 gcggtgtatt cggtcacgca atgggcggct cagcgcgcgc gcaccggcgg cggccctacg
     1561 ttgatcgagc tcgtcaccta tcgcgcgtcc gcgcattcga ccagcgacga tccgtctcgc
     1621 taccgtccca aggacgacta cgaacactgg ccgctcggcg acccggtaga tagattgaag
     1681 aatcacctga tcgcgcgcgg cgagtggagc gagaagcagc acaaggagct gcacgccgag
     1741 ctcgacgccc aggtagtcac ggcctggaag gaagccgtgt cgttcggcgc gctcaacgaa
     1801 ggcccgcgcc tcgaccgcca cctgatgttc gaggacgtct acaaggagtt tccggacaac
     1861 ctgcgccggc agagcgagca gctcgccgaa gagatcaaac tcaacgccga gctcgggctc
     1921 gtcgcgaagg tggattgatc atggcgcaaa tgaacatgat ccaggccctg aactccgcca
     1981 tggacgtgat gatggcgcgg gatccttccg tcgtcgtcat gggcgaagac gtgggctatt
     2041 tcggcggcgt gttccgtgtc accgacggcc tgcagagaaa atacggcgaa caccgcgtgc
     2101 tcgactcgcc gatttccgag ggcggcatcg tcgccacggc catcggcatg gccgtcaatg
     2161 gcctcaagcc cgtcgccgag atccagttcg ccgactacat ctatccggcc gtggaccaga
     2221 tcgcgagcga gcttgcgcga atccgatatc gcagcgccgg agatttctcg tcgccagtgg
     2281 tgatccgcgc gccctgcggc ggcggtatcc gcggcggtca gacgcattcg cagagtcccg
     2341 aggcgatctt cacgcacatc tgcggcgtga aggtcgtgat gccgagcaat ccctacgacg
     2401 cgaagggtct gctcatcgcg gccatcgaag acgatgaccc gatcatcttc ttcgagccga
     2461 agcgcatcta caacggtccg ttcgacggcg accctaacaa gcctgcggtg ccgtggagca
     2521 cacacgccaa gggcaacgtg cccgagggtc actacaaagt gccgatcggc aagtgcgaca
     2581 tcgtgcgtcc cggcgagcag gtcaccatcg tcacctacgg caccatggtg tacgtgtgcg
     2641 aggccgcggc gaagaaactc aacatcgacg ccgagatcat cgacgtgcga accatgtcgc
     2701 cgctcgacat cgatcccatc tgctcgtccg tcaagaagac cggccgatgc gtggtcgctc
     2761 acgaagccac gcgtttctcg ggctttggcg cggagctctc ggccagcatc caggaagaat
     2821 gtttctggta cctcgaggcc ccgatccagc gagtggccgg ctgggatacg ccgtatccgc
     2881 acgcgttcga atgggag
//
LOCUS       DATDEU010000544         3095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_396213, whole genome shotgun sequence.
ACCESSION   DATDEU010000544 DATDEU010000000
VERSION     DATDEU010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396213"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..605
                     /locus_tag="VIV63_13010"
     CDS             225..605
                     /locus_tag="VIV63_13010"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEY6125565.1"
                     /translation="MKHSPWRKLVLGILSVAIFNMGMVGAASAAIADTASLVTVDRAA
                     DMSVVRDHLDRADVRAKMEEMGIEATAVDSRLAALNDRELHQLAADMQNAPAGGDAVW
                     IVGAVFIVLIILELTGVIDIFKKA"
     gene            616..1653
                     /locus_tag="VIV63_13015"
     CDS             616..1653
                     /locus_tag="VIV63_13015"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HEY6125566.1"
                     /translation="MKKRAAVVVARFAFLLATAGLLSACVTREPFIPANLEPGAQRTV
                     ELDATPFFPQDKYQCGPAALATVLSASGATTTPDALVPVVYLPARKGSLQVEMLAAPR
                     KFGRLSYALSRNLVSVLEELGAGRPVLVLHNYGLPFWPRWHYAVVIGYDAATDKFILR
                     SGKKRRTEWRARTFMVAWHNGGRWAMVALRPGETPASADPTLYLEAAADFERGASPAD
                     SRLAFDAAVQRWPAEPVAWIGRGTAEYRAGNVANAARDYAAALRIDPDNVGARNNLAQ
                     SLLDLGCPALAREQLAPVDAEALKSPLKEAVLDTRQHIEAAVVATPVSEASRCAPQFQ
                     FLSPDAYTPHM"
     gene            1929..2762
                     /locus_tag="VIV63_13020"
     CDS             1929..2762
                     /locus_tag="VIV63_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY6125567.1"
                     /translation="MFVTRFRHLFLVGFLCAAGAAHAQTAGTVTLQANATSATGSMTP
                     RLTWSTNPVATSCRASGGWSGNKAVSGSETLAAINASTNYTLTCSWGTGSTTVNWVAP
                     TTNTDGSALTNLAGFRVYYGTSATALSSSTTVTDVTRRSTTVSSLAPGTWYFAVRAFN
                     TNNAESDNSNVATKTVTGASAARTVNITITAPTPVPPTPTPPPTGSLRTTSVDVYDVL
                     QQSNGTWAPRAIVGRIALGRPCSTAFTATGGYYQINKADVTRNSTNPWSNIFVARCAA
                     S"
     gene            complement(2766..>3095)
                     /locus_tag="VIV63_13025"
     CDS             complement(2766..>3095)
                     /locus_tag="VIV63_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6125568.1"
                     /translation="AQNQEYLQTIKRFLQVSPGSTVLLRGHVDNARVNEFRQSGGDQL
                     VQSMALKAMELSRQRAMAVHDALLAKFPEIDKTRVEAVGRGWEEPTGTTDAAQNRRVE
                     VQWFTIE"
BASE COUNT          522 a         1035 c          956 g          582 t
ORIGIN      
        1 cgccgccatt catggcgaga agcagcacga gcccggcgag ctttgagact gtggctagtt
       61 tcattgttaa attccctgtc tgcgcgacct gacaacgctc tgaatggcga taacgcgacg
      121 aagggtaagt tacttccttc acgctctcct gccgtcctac attccagaac tccggtgttg
      181 ctaacctgcc tcccggtttg ctcaactctc ggaggtctct ttacatgaaa cattcaccgt
      241 ggcggaaact cgtcctcggc attctttccg tggccatctt caacatgggg atggttggcg
      301 ccgcctccgc agcaattgcg gataccgcga gcctggtcac cgttgaccgc gccgctgaca
      361 tgagcgtcgt gcgcgaccat ctcgatcgcg ccgacgttcg cgcgaagatg gaggaaatgg
      421 gaatcgaagc gacggccgtc gacagccgcc tggccgcgct caatgaccgg gagctgcacc
      481 agctggcggc cgacatgcag aacgcgcccg cgggcggtga tgcagtctgg atcgttggcg
      541 cggtattcat cgtgctgatc atcctcgaac tgactggcgt catcgacatc ttcaagaagg
      601 cctgagcggt ccccgatgaa gaagcgcgca gccgtcgtgg ttgcgcgctt cgccttcctt
      661 cttgccacag ccggattgct gtccgcgtgc gtcacacgcg agccgttcat ccccgccaat
      721 ctcgagccgg gtgcgcaacg caccgtggag ctcgacgcca cgcccttctt tccccaggac
      781 aagtaccagt gcggaccggc cgcgctcgcg actgtgttgt ccgcttccgg cgcgacgacg
      841 acgcccgatg cgctggtgcc cgtcgtctat ctgcccgcgc gcaaggggag tctgcaggtg
      901 gagatgctgg cggcgccgcg caagttcggc cggctctcgt atgcgctgtc gcgaaacctg
      961 gtctcggttc tagaagagct gggcgcaggc aggcccgtgc tcgtgcttca caactacggt
     1021 ttgccgttct ggccccgatg gcactacgcc gtggtcatcg gctacgacgc ggcgaccgac
     1081 aagttcatcc ttcgctctgg caagaagcgg cgcacggaat ggcgcgcgcg aactttcatg
     1141 gtcgcgtggc ataacggtgg ccgctgggcg atggtcgcgc tacggcccgg cgagacaccg
     1201 gcgagcgcgg accccacgct ctatctggaa gcggccgcgg atttcgaacg cggcgcgagt
     1261 cccgctgatt cacgcctggc attcgacgcg gccgtgcagc gctggcccgc ggaaccggtg
     1321 gcgtggatcg ggcgaggcac cgccgagtat cgcgccggca acgtggcgaa tgcggcgcgc
     1381 gactacgccg cggcgctacg catcgatcct gacaacgttg gcgcgcggaa caatctcgcg
     1441 cagtcgttgc tcgacctcgg atgtccggcg ctggcgcgcg agcagctcgc gcccgtggat
     1501 gcggaggcgc tgaagtcccc attgaaggaa gccgttctcg acacgcgcca gcacatcgaa
     1561 gcggcggtcg tcgccactcc cgtaagcgag gcgagcaggt gcgcaccgca gttccagttt
     1621 ttgtcgccag atgcgtacac cccgcatatg tgacagaacg cacttcgtcc gtcgcgaatt
     1681 ttccgcgata gctcttgtat tactcgaaag ggcccccgct tttgcctgcg tgcgagggtt
     1741 tcttccagag attcggcgcg attgtcgctg cacgccgccg gttggcagtt tttaccgacg
     1801 cacgtgtcgc gctcgggcgt cagggttttc cttttttcaa ctcagcagaa accgcgcctc
     1861 cgtttgcggc acctgacccg cgtgcccaat ctgcccctca cgttttcatg actcgtgggg
     1921 tcggtttcat gttcgtcact agatttcgtc atctgttcct cgttggattc ctgtgtgccg
     1981 cgggtgccgc gcacgcgcaa accgcgggaa ccgtgactct gcaggccaac gccacttccg
     2041 ccaccggaag catgacgccg cggctgacgt ggtcgaccaa tccggtcgcc acgagctgcc
     2101 gcgcgagcgg cggctggtcc ggaaacaagg ccgtctcggg atccgagacg ctcgccgcca
     2161 tcaacgccag cactaactac acgctgacct gttcctgggg taccggatcg acgacggtca
     2221 actgggttgc tcccacgacc aacaccgacg gttcggcgct gaccaacctc gcgggcttcc
     2281 gcgtgtatta cggcaccagc gccacggcgc tctccagcag caccacggtg accgacgtga
     2341 cacgccggtc gacgacggtc agctcgctgg cgccgggaac ctggtacttc gcggtacgcg
     2401 cgttcaatac gaacaacgcg gaatccgaca acagcaacgt ggccacgaag acggttaccg
     2461 gtgcttcggc tgctcgcacg gtgaacatca ccatcaccgc gccgacgccc gtgccgccga
     2521 cgccgacacc gccgcccacc ggcagcctgc gtacgacttc cgtggacgtc tacgacgtgt
     2581 tgcagcagtc gaacggcacc tgggcgccgc gagccatcgt cggccgcatc gcgctcggtc
     2641 gcccgtgcag caccgcgttc accgcgacgg ggggttacta ccagatcaac aaggccgatg
     2701 tgacccgcaa cagcacgaac ccgtggtcca acatcttcgt ggcgcgctgc gcggcgagct
     2761 gagggttact cgatagtgaa ccactgcacc tcgacgcggc gattctgcgc agcgtcggtg
     2821 gtgccggtgg gttcttccca gccacgaccc acggcttcca cgcgggtctt gtcgatctcc
     2881 gggaatttcg cgagcaacgc gtcatggacg gccatggcgc gttgtcgtga aagctccatg
     2941 gccttcagcg ccatgctctg caccagttga tcaccacctg actgacggaa ttcgttgacg
     3001 cgcgcgttgt ccacgtgacc gcgcagcagc accgtcgagc cggggctgac ctgcaggaat
     3061 cgtttgatgg tctgcaggta ctcctgattc tgggc
//
LOCUS       DATDEU010000545         5493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1128819, whole genome shotgun sequence.
ACCESSION   DATDEU010000545 DATDEU010000000
VERSION     DATDEU010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128819"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VIV63_13030"
     CDS             complement(<1..276)
                     /locus_tag="VIV63_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY6125569.1"
                     /translation="MALRLRVISDHRHRLGDKNTFVFGVSGGSIGRSAENDWVLPDDM
                     RYVSGRHARIVYHKGRYMLQDTSSNGTYVNDNERPLGNQNPHELKSGD"
     gene            381..695
                     /locus_tag="VIV63_13035"
     CDS             381..695
                     /locus_tag="VIV63_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HEY6125570.1"
                     /translation="MFVNQGKVYEVYARKVGQGRLFGFIEVEELVFGERSTVVLDPGE
                     ERIKSEFAGVKCTMLPMHSILRIDEVKKQGVSKVSAFEGSNVSAFPMSMYTPASDPGT
                     KK"
     gene            832..4746
                     /gene="purL"
                     /locus_tag="VIV63_13040"
                     /gene_synonym="purI"
     CDS             832..4746
                     /gene="purL"
                     /locus_tag="VIV63_13040"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY6125571.1"
                     /translation="MLELPGASAVSEFRIAKLRPALEALHPGIGAISARFVHFVDLSR
                     DLDARETALLDRLLTYGPREDAPARSASAATEIIVVPRFGTISPWSSKATDIARVCGL
                     DAVLRIERGIVWRIDVAKKLSRDEAARLAAPLFDRMTETALHERGDAARLFVKEQTRA
                     LRTVSLASGRGALVAANAELGLALSDDEIDYLVKNFAALGRDPTDVELMMFAQANSEH
                     CRHKIFNADWIIDGERQRKSLFAMIRNTHEKHPQGVLSAYRDNAAVMEGALGKRYFPN
                     PENDVYGAVDEPIDILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGL
                     TGFSVSHLRIPGFEQPWEQTPGEQLGKPDRIVSALDIMIEGPIGGAAFNNEFGRPNIC
                     GYFRTFEQAAQGDPAGRVRGYHKPIMIAGGLGNVRRNHVEKCEIPVGTKIIVLGGPAM
                     LIGLGGGAASSVNSGASSADLDFASVQRGNPEIQRRAQEVIDRCSARWSLNPILLIHD
                     VGAGGLSNAVPEAVAHTAGRGARIDMGAIPNDEPGMSPMELWCNESQERYVLLIDAAR
                     LQDFASIALRERCPYAVLGDIDDTGVLVLEDRKNGGTPVNLPLEVLLGKAPKMLRDVR
                     SVTPPQAALAAEKLDLRDAAYRLLRFPAVADKTFLISIGDRTVGGMISRDQMVGPWQV
                     PVADVAVTIADYFGHAGEAMSMGERTPVAVLDAPASGRLAVGEALTNILAADIGALSN
                     VKLSANWMAACGEPGEDAALYATVRTVGEELCPALGIAIPVGKDSLSMKTVWGDATHR
                     KSVVAPVSLIISAFAPVGDVRKTWTPQLRADLGATELLLVDLGQGANRLGGSALAQVF
                     GELGSTPADLASPTLLINLASALAELRAQNLVLAYHDRSDGGVFATLVEMAFAGHCGI
                     DIKLPAGAGATGAAGALFCEELGVVLQVKAEHAATVRGIFARHGLGECTHAIGAATRE
                     MRVIVQAGATRIDESWADLRRAWSETSFRMRERRDEPSCAHEEFAAACDTGAPGLNVA
                     LTFNPDEDIAAPFIGGARPKVAVLREQGVNSQIEMAAVFERVGFEPHDVHMSDVLSGR
                     VTLDGFRGLVACGGFSYGDVLGAGEGWAKSILYHAGAREQFQRFLDRADTFTLGVCNG
                     CQMFAALKEIIPGAAAWPRFVRNRGEQFEGRFSLVELQPSPSIFLAGMDGSMLPIAVA
                     HGEGRAEFANDADAAAFSKGGLVSARYIEGNRRIATQYPGNPNGSPFGIAAITSEDGR
                     VTLTMPHPERSFRYAQNSWRPDGAGEYSGWYRMFANARRWVG"
     gene            complement(4815..>5493)
                     /locus_tag="VIV63_13045"
     CDS             complement(4815..>5493)
                     /locus_tag="VIV63_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125572.1"
                     /translation="GAMYFGDERETTRNCPHCHALSRMSPTALPTFDELLLQKPRSVG
                     LVMRCHACTAPVFLRFAVRAYASNRVELAPQAFDVERAIEKFAFNYVPECAEIYFREA
                     LICYSHNQFQAFASMCRRAAGAAFQDLGNPTKLRLFDSLSDVREMAEIDEETFAVVMR
                     IIFAGHADPLLDLPFINTYQAAVLLEVMKDFLYQAYVRKGRLQHAMKVRRFFVEEHAA
                     RRAVSDE"
BASE COUNT          976 a         1767 c         1785 g          965 t
ORIGIN      
        1 gtcgccgctc ttcaactcgt gcgggttctg gttgccgagc gggcgttcgt tgtcgttgac
       61 atacgtgccg ttgctgctgg tgtcctgcag catgtaacgg cccttgtgat aaacgatgcg
      121 cgcatgccgt cccgacacgt atctcatgtc gtctggcaat acccaatcgt tctcggccga
      181 gcgtccaatg cttccgcccg agacgccaaa gacgaacgta ttcttgtcgc ccaatcgatg
      241 gcgatgatca ctgatgacgc gcaaccggag cgccatgtac aaccctctgg gcctagcttc
      301 ccgcagccca tgcgtgtatg aaggggcagg ttaccgcccc cctagtccgc tcgatggcat
      361 ccagtcacat tttcaagatt atgttcgtga accaaggcaa ggtttacgag gtttatgccc
      421 gtaaggtggg ccagggccgc ctgttcggat tcatcgaggt cgaggagctg gtgttcggcg
      481 agcgatccac cgtggtcctg gacccgggcg aggaacgcat caaatccgaa ttcgcaggcg
      541 tcaagtgcac gatgctgccc atgcattcca tcctgcgcat cgacgaagtc aagaagcagg
      601 gcgtgagcaa ggtcagcgcc ttcgaaggca gcaacgtctc ggcgttcccc atgtcgatgt
      661 acacgccggc cagcgatccc gggaccaaaa agtaggaaac ggggatctga aaaaggggac
      721 agatctattt attgaccggg cgcgctcgta aatgtagatg acatcgacca ataaatagat
      781 ctgtcccctt tttcagatct gtccccattt cccgtacaat tcgcgccctc catgctcgag
      841 ctccctggcg cctccgcagt ctccgaattt cgcatcgcaa aactgcgtcc cgcgctcgag
      901 gctcttcacc ccggcatcgg cgccatcagc gcgcgcttcg tccacttcgt cgatctctcg
      961 cgcgatctcg atgcacgtga aaccgcgctg ctcgaccggc tcctgaccta cggcccgcgc
     1021 gaagacgcgc cggcgcgcag cgcctcggcc gccaccgaaa tcatcgtcgt gccgcgcttc
     1081 ggcaccatct cgccctggtc gagcaaagcc acggacatcg cgcgggtgtg cggactcgac
     1141 gccgtccttc gcatcgagcg cggcatcgtc tggcgcatcg acgtcgcgaa gaagctgtcg
     1201 cgtgatgaag ccgcgcgcct ggcagcgccg ctgttcgatc gcatgaccga gacggcattg
     1261 cacgaacgcg gcgacgccgc gcgtctcttc gtcaaggaac aaacgcgggc gctgcgcacc
     1321 gtctcgctcg ccagcggacg tggcgcgctg gtcgccgcga acgccgagct cggccttgcg
     1381 ctgtcggacg acgaaatcga ctacctggtg aaaaatttcg ccgcgctcgg gcgcgacccc
     1441 acggacgtcg aactcatgat gttcgcgcag gcgaactccg agcactgccg ccacaagatt
     1501 ttcaacgccg actggatcat cgacggcgaa cgccagcgca agtcgctgtt cgcgatgatc
     1561 cgcaacacgc acgagaaaca tccgcagggc gtgttgtcgg cttatcgcga taacgccgcc
     1621 gtgatggaag gcgcgctggg caagcgctat ttcccgaatc ccgagaacga cgtctacggc
     1681 gcggtcgacg agcccatcga catcctcatg aaggtcgaga cgcacaacca tccgacggcg
     1741 atctcgccgt tcccgggcgc ggccacgggg tcgggcggcg agatccgcga tgagggcgcg
     1801 acgggacgtg gcgcgaagcc gaaagccggt ctcacgggat tctccgtgtc gcacctgcgc
     1861 attccgggct tcgagcagcc gtgggagcaa acgccgggtg agcagttagg caagccggac
     1921 cgcatcgtct cggcgctcga catcatgatc gaagggccga tcggcggcgc cgcgttcaac
     1981 aacgaattcg gtcgccccaa catctgcggc tacttcagga ctttcgaaca ggcggcgcag
     2041 ggcgatcccg ccggccgcgt tcggggttac cacaagccca tcatgatcgc cggcggcctc
     2101 ggcaacgtgc gccgcaatca cgtggagaag tgcgagattc ccgtggggac gaagatcatc
     2161 gtgctcggcg gacccgcgat gctcatcgga cttggcggtg gcgccgcgtc gtcggtgaac
     2221 agcggggcgt cgagtgccga tctcgacttc gcatccgtgc agcgtggcaa tcccgagatc
     2281 cagcgccgcg cgcaggaagt catcgaccgt tgctccgcgc gatggagcct caatcccatt
     2341 ctgctgattc acgacgtggg cgccggtggc ctgtcgaacg cggtgcccga agccgtggcg
     2401 cataccgctg gccgcggcgc gcgcatcgac atgggcgcga ttccgaacga cgagcccggc
     2461 atgtcgccca tggaattgtg gtgcaacgaa tcgcaggagc gctatgtcct gctgatcgac
     2521 gccgcgcgac ttcaggattt cgcgtcgatt gcgctgcgtg aacgctgccc gtatgccgtg
     2581 ctcggcgaca tcgacgacac cggcgtgctg gtgctcgaag accggaagaa cggtggaact
     2641 ccggtgaatc tgccgctcga agtgttgtta ggcaaggcgc cgaagatgtt gcgtgacgtg
     2701 cgcagcgtga cgccgcccca ggccgcattg gcggcggaaa aactcgatct gcgcgacgcc
     2761 gcgtaccggc tgctgcggtt cccggccgtc gccgacaaga ccttcctgat ctcgattggc
     2821 gaccgcaccg tcggcggcat gatcagccgc gaccagatgg tcgggccctg gcaggtgccg
     2881 gtggccgacg tcgccgtgac catcgctgac tacttcgggc acgccggcga ggcgatgtcg
     2941 atgggcgaac gcacgccggt ggccgtgctc gatgcgcccg cttccggccg tctcgcggtg
     3001 ggtgaggcgc tcaccaacat tctcgccgcc gacatcggtg cgttgtcgaa cgtgaagctg
     3061 tcggccaact ggatggcggc gtgtggcgag cctggcgaag acgcggcgct gtacgccacc
     3121 gtgcgcacgg tgggtgaaga actgtgtccc gcgctgggca tcgccattcc cgtcggcaaa
     3181 gattccctgt cgatgaaaac agtctggggc gacgccaccc accgcaaatc ggtggtcgcg
     3241 ccggtgtcac tgatcatctc cgcgttcgcg ccggtgggcg atgtgcgcaa gacgtggacg
     3301 ccgcagctgc gcgccgatct cggtgcgacc gagttgttgc tcgtggatct gggccagggc
     3361 gctaaccgcc tcggtggctc cgcgctcgca caggttttcg gcgagttggg cagcacgcct
     3421 gcggatctgg cttcgccgac gctgctgatc aacctggcgt ccgcgctcgc cgagctgcgc
     3481 gcgcaaaacc tcgtgctggc ctatcacgat cgttccgatg gcggcgtatt cgcgacgctg
     3541 gttgaaatgg cgttcgccgg acattgcggg atcgacatca aactacccgc gggcgccggc
     3601 gccacgggcg cggccggagc cctgttctgc gaagaactcg gcgtcgtgtt gcaggtgaag
     3661 gcagaacacg cggcgaccgt gcgtggcatc ttcgcgcggc acggactcgg tgagtgcact
     3721 cacgcgatcg gagccgcgac acgcgagatg cgcgtgatcg tgcaggcagg cgccacgcgc
     3781 atcgacgaat cgtgggcaga cctgcgccgc gcctggtcgg aaacttcgtt ccgcatgcgc
     3841 gagcgccgcg acgagccatc ctgcgcgcac gaggaattcg cggcggcctg cgataccggc
     3901 gcaccgggtc tcaatgtcgc gctcacgttc aatccggatg aagatatcgc agcaccgttc
     3961 atcggcggcg ctcgcccgaa agtggcggtg ctgcgcgagc agggcgtgaa cagccagatc
     4021 gagatggcgg cggtgttcga acgcgtgggc ttcgagccgc acgacgtgca catgagcgac
     4081 gtgctctcgg gtcgcgtgac gctcgacggc ttccgcgggc tcgtcgcgtg cggcggcttc
     4141 tcttacggcg acgtgctcgg cgccggcgag ggctgggcga agtccatcct ttatcacgcg
     4201 ggtgcgcgcg agcagttcca gcgttttctc gatcgcgcgg acacgttcac gctcggcgtc
     4261 tgcaacggct gccagatgtt cgccgcgctc aaggaaatca ttccgggcgc cgccgcatgg
     4321 ccgcgcttcg tgcgtaatcg cggcgagcag ttcgaggggc gtttctctct ggtcgaactg
     4381 caaccgtcgc cgtccatttt cctggccggc atggacggat ccatgctgcc cattgccgtg
     4441 gcgcatggag aagggcgtgc cgagttcgcg aatgacgccg acgcggcggc gttctcgaag
     4501 ggtggcctgg tctcagcgcg ttacatcgaa ggcaaccggc gcatcgccac acagtacccg
     4561 ggcaacccca acggttcgcc tttcggcatt gcggccatca ccagtgaaga cggccgcgtg
     4621 acgctcacga tgccgcaccc ggagcgttcg ttccgctacg cgcagaactc atggcgaccc
     4681 gacggcgccg gcgaatattc gggctggtac cgcatgttcg cgaacgcgcg acgatgggtg
     4741 gggtagaaaa gggcaagtag aaaaaagggg acagatctat tttttcggaa aaaaatagat
     4801 ctgtcccctt ttttctactc gtcgctgacc gcgcgccgcg cggcgtgttc ctcgacgaag
     4861 aaccgccgca ccttcatcgc gtgttgcagg cggcccttgc gcacgtacgc ctggtagagg
     4921 aaatccttca tcacctcgag caacaccgcc gcctggtagg tgttgatgaa gggcaggtcg
     4981 agcagcgggt ccgcgtggcc cgcgaagatg atgcgcatca ccacggcgaa agtttcttcg
     5041 tcgatctccg ccatctcgcg cacgtcggac agcgaatcga acaggcgcag cttggtgggg
     5101 ttgccgagat cctggaacgc agcgcccgcg gcgcgccggc acatcgacgc gaacgcctgg
     5161 aactggttgt gcgagtagca gatgagcgcc tcgcggaagt agatctccgc gcactccgga
     5221 acgtagttga acgcgaactt ctcgatggcg cgttcgacgt cgaaggcctg cggcgccagc
     5281 tcgacgcggt tgctggcata cgcgcgcacg gcgaagcgca ggaacacggg cgccgtacac
     5341 gcgtggcaac gcatgaccag cccgaccgag cgcggcttct gcaacagcag ttcgtcgaag
     5401 gtgggcaacg ccgtcgggct catgcgcgac aacgcatggc aatgcggaca attgcgggtc
     5461 gtctcgcgct cgtcgccaaa atacatggcg cca
//
LOCUS       DATDEU010000546         3424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1564960, whole genome shotgun sequence.
ACCESSION   DATDEU010000546 DATDEU010000000
VERSION     DATDEU010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564960"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3424)
                     /locus_tag="VIV63_13050"
     CDS             complement(<1..>3424)
                     /locus_tag="VIV63_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEY6125573.1"
                     /translation="SRREIVLRADPQFAPGTDYHVSVRAQGLTGVAPVAKPFEFKVKT
                     LGVNFDVRTYGLDVQDNRNELMTLRGAVLTADTEDRERIEKIVTATLDGKPLPIVWTA
                     GENQYGFTISDIVRKSEEQEVIVKWNGAPLNVENEGSQSWRIPALDEFAVTRTEAVQL
                     NDQRQIQVQFSDALDARQELKGQVRLSQGEFTTSISGNLLTLYLNEDVVGEVTLTLET
                     AVRSRSGRPLVGLREFKLEFTNTKPQVRFVGKGVILPDATTLSVPFEAVSARAVRVTA
                     LQVFEANIPQFLQVNPLGGSQQLGRVGRVLWRKTITLASPVPGKWTRYDLDVTELMRK
                     HPGGLFQLTLSLAPSDALYDCPNDSETAPPEDPTPTSAEDGDTHDYSNWDYYQEEYYD
                     GEINWNERDDPCKPAYYRYGRNIRAARNLLASNIGLIAKRAQRGKLLAVATALDSAKP
                     LAGVKIDAMSFQNQVMASGRTDSQGMLELDPRGNVFALIADDGGRKGYLRVANGVALP
                     VSHFDVGGETVVAGLKGFLYGDRGVWRPGDSIYLTFALQDRSKTLPPNHPVTVELRNP
                     RGQLVQTLTNSKPVGQFYAFELKTPADAPTGDWNVVALVGGATFGKTLKVETVMPNRL
                     KIDLDLGEKSVVESSPLKGMVNAQWLSGATAANLRAAVEVRLSSAPTRFTRNADFVFD
                     DPARSFSGSPITVYDGALDADGKAKIEKRLDLPRDVPGMLNATFITRVFERGGAFSIN
                     RETRTVAAFERYVGLKLPKGDAARDMLLTDTSHVVELATLNVDGVPVSIPRLQVSIYK
                     VQWRWWWDSSGDSLAQYAQGESTSMIKQDTIASKDGRAQWKFEVKYPEWGRYLVRACD
                     LDGGHCTGKVFYIDWPSWAGAARDQSGPAANILMLTSDKQEYRVGENAFVQLPEASQG
                     RALLTLESGSAILEQRWIEAKPGANRISIPITAGMAPGIYAAVTMVQPHAGKTNDRPI
                     RLYGVIPLKVTDPKSNLAPQVITAAEWAPQSKASITVSEKTGRAMDYTLAVVDEGLLG
                     LTGFKTPNLHGEFYKREALGVSTWDLFDEVAGAYGGQLDRLLALGGSDASSATNPDDA
                     KSRFPPVVRFLGPFTLKAGEKRSHTVELPQYVGAVRVMVVAGDGSAYGSAEKS"
BASE COUNT          551 a         1070 c         1163 g          640 t
ORIGIN      
        1 ggatttctcc gccgagccgt aagcgctgcc gtcgccggcc accaccatga cgcgcacggc
       61 gccgacgtac tgaggcaact ccacggtgtg cgaacgtttc tcgccggcct tgagcgtgaa
      121 tgggccgagg aaacgcacca cgggcgggaa gcgcgatttg gcgtcgtcag gattggtagc
      181 cgaggaggca tcgctgccgc cgagcgcgag cagccggtcg agctggccgc cataggcgcc
      241 cgcgacttcg tcgaacaggt cccacgtgga gacgccgagc gcctcgcgtt tgtagaactc
      301 gccgtgcagg ttcggggtct tgaagccggt gagccctaac aagccttcgt cgacgaccgc
      361 aagcgtgtag tccatcgcgc ggccggtctt ttcactcacc gtgatgctgg ccttcgactg
      421 cggcgcccat tcggccgccg taatcacctg cggcgcgaga ttcgacttcg gatcggtcac
      481 cttcaacggg atcacgccgt agagccggat cggccggtcg ttggtcttgc cggcatgcgg
      541 ctgaaccatg gtgaccgcgg cgtaaatgcc gggcgccatg cccgcggtga tcggaatcga
      601 aatgcggttg gcgccgggtt tcgcttcgat ccagcgctgc tcgaggatgg cgctgccgct
      661 ttcgagggtc agcaacgcgc ggccttgcga ggcttcgggc aattgcacga acgcgttttc
      721 accgacgcga tattcctgct tgtcggacgt gagcatgagg atgttggcgg cgggacccga
      781 ctgatcgcgg gccgcaccgg cccacgaggg ccagtcgatg tagaacacct tgccggtaca
      841 gtggccgccg tcgagatcgc aggcccggac caggtaccgg ccccattccg ggtacttcac
      901 ctcgaatttc cactgcgcgc ggccgtcctt cgacgcgatg gtgtcctgtt tgatcatgga
      961 ggtgctttcg ccctgagcgt actgcgccag cgaatcgccg ctcgagtccc accaccagcg
     1021 ccactgcacc ttgtagatag agacctggag ccgcggaatc gacaccggca cgccatcgac
     1081 gttcagcgtc gccagttcca ccacgtgcga ggtgtcggtg agcaacatgt cgcgcgccgc
     1141 gtcgcccttg ggcagcttga ggccgacgta gcgctcgaat gcggcgacag tacgtgtctc
     1201 gcgattgatg ctgaaggcac cgccgcgctc gaatacgcgc gtgatgaacg tggcgttgag
     1261 catgccgggc acatcgcgcg gcagatcgag acgcttctcg atcttcgcct tgccgtcggc
     1321 gtccagcgcg ccgtcgtaca ccgtgatcgg cgaacccgag aacgaacgcg ccggatcgtc
     1381 gaagacgaaa tccgcgttgc gcgtgaagcg cgtgggcgcg ctcgacaacc gtacttcgac
     1441 ggcggcgcgc aggttggccg ccgtggcgcc ggacagccac tgcgcgttca ccatgccctt
     1501 gagcggcgaa ctttcgacga cgctcttttc gccgagatcg agatcgatct tgaggcggtt
     1561 gggcatcacg gtctccactt tcagcgtctt gccaaacgtg gcgccgccga cgagggcgac
     1621 cacattccag tcgccggtgg gtgcgtcggc gggggttttc agttcgaagg cgtagaactg
     1681 cccgacgggc ttcgagttgg tgagcgtctg cacgagctgg ccacgcggat tgcgcagctc
     1741 gacggtgacc gggtggttgg gaggcagcgt cttcgagcga tcctgcagcg cgaaggtgag
     1801 gtagatggag tcgcccggac gccagacgcc gcggtcgcca tagagaaagc ccttgaggcc
     1861 cgcgaccacg gtttcgccgc ccacgtcgaa gtggctgacc ggcagtgcga cgccattcgc
     1921 gacacgcagg taacccttgc ggccgccgtc atcggcgatg agcgcgaaca cgttgccgcg
     1981 ggggtcgagc tcgagcatgc cctggctgtc ggtgcgaccg ctcgccatga cctggttctg
     2041 gaaactcatg gcgtcgatct tcacgccggc gagcggtttg gcgctgtcga gcgccgtcgc
     2101 aaccgcgagt agtttgccgc gctgcgcgcg cttggcgatc aggccgatgt tggaggcgag
     2161 caggttgcgc gccgcacgga tgttgcggcc gtaacggtag taggccggct tgcagggatc
     2221 gtcgcgttcg ttccagttga tctcgccgtc gtaatactcc tcctggtagt aatcccagtt
     2281 cgagtagtcg tgggtgtcgc catcttcggc gctcgtgggc gtgggatctt cgggcggcgc
     2341 ggtttcggaa tcgttcgggc agtcgtagag cgcatccgag ggcgcgagcg acaacgtgag
     2401 ctggaacaga ccgccgggat gcttgcgcat gagctcggtg acatcgaggt cgtagcgagt
     2461 ccacttgccg ggaaccggcg aggccagcgt gatggtcttg cgccacaaca cgcggccgac
     2521 acggccgagc tgctgagagc caccgagcgg gttcacctgc aggaactgcg ggatgttggc
     2581 ttcgaaaacc tgcagcgccg tgacgcgcac cgcgcgcgcg ctgacggcct cgaacgggac
     2641 gctgagcgtg gtggcgtcag gaaggatcac gcccttgccg acgaagcgca cctgcggctt
     2701 ggtattggtg aactcgagct tgaattcgcg cagaccgacc agcggtcgac ccgaacggct
     2761 gcgcacggcg gtctcgagcg tcagcgtcac ttcgcccacg acgtcttcat tgagatacag
     2821 cgtgagcagg ttgccgctga tcgaggtcgt gaactcgcct tgtgacagtc ggacctgccc
     2881 cttcagttcc tgccgcgcgt cgagcgcatc ggagaactgg acctggatct gtcgctggtc
     2941 gttgagttgc acggcctcgg tccgcgtgac cgcgaactca tcgagagccg ggatgcgcca
     3001 gctttgcgag ccttcgttct ccacgttcag cggtgcgccg ttccatttga cgatgacttc
     3061 ctgttcctcg ctcttgcgca cgatgtcgct gatggtgaag ccgtactgat tttcgccggc
     3121 ggtccagacg atgggcagcg gcttgccgtc gagcgtggcg gtgacgatct tctcgatgcg
     3181 ctcgcgatcc tcggtgtcgg cggtgaggac ggcgccgcgc agcgtcatga gctcgttgcg
     3241 gttgtcctgc acgtcgaggc cataggtgcg aacgtcgaag ttgacgccca gcgtcttcac
     3301 cttgaactcg aaaggcttgg caacgggtgc gacgccggtg agtccttgcg cgcgcacgct
     3361 cacgtggtag tccgtacccg gcgcgaattg cggatccgcg cgcagcacga tttcacggcg
     3421 gctt
//
LOCUS       DATDEU010000547         6487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2002693, whole genome shotgun sequence.
ACCESSION   DATDEU010000547 DATDEU010000000
VERSION     DATDEU010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002693"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VIV63_13055"
     CDS             complement(<1..1325)
                     /locus_tag="VIV63_13055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6125574.1"
                     /translation="MGFAASSLAAGRRRKYVNVGVGSRSRMYLTAITKTFAEGNELVG
                     ICDTNAGRLDVAAAFVAPNGSKPKKYAATDFDKMLKDLKPDCVVVTCPDAFHDDYIVR
                     ALDAGCDCITEKPLTTTPEKAQRILDACKRNNRHVRVLFNYRYSPPRTQVKDLLMSNA
                     IGDVLSVDFHWLLNTSHGADYFRRWHSQKEISGGLMVHKATHHFDLVNWWLGSDPEIV
                     MAYGKKEFYTPAMAKRMGLTSHHERCRTCPEKEKCTFYLDLAADKNFKSLYLDNEQYD
                     GYLRDRCVFRPDINIEDTMNVIVKYKMGATLSYSLNACNSWEGYTIAFNGTKGRIEHE
                     IVEGGAISAGATNYQGTGGDRERTRIIPLRGKAQDLEVWTGTGGHGGGDNVMLTELFG
                     TAEPDKYKRAADERGGLYSILIGAAANQCFKTGNSVKIDELVSGLSSPV"
     gene            1502..3742
                     /locus_tag="VIV63_13060"
     CDS             1502..3742
                     /locus_tag="VIV63_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125575.1"
                     /translation="MYLRALVFLTTTLVVSQLAVAAPIDRHALVTRHNPTITTVDRAA
                     PFMVGNGNLAFTADITGLQTFPEQYSALVPLMTQAQWAWHSFPNPQKFSLAQAEVPID
                     VRGKKQKYPYLTDWEQAKRPEIQWLRENPHRFSLARLGLHLVNADGKIAAFGELTATR
                     QTLDMWTGRLVSSFEFNGTPVEVETSVHPDRDLLIVRLRSTLLADGRLGFNLRFPGVG
                     AKLNPDPADWTRPEAHTTQEVTRNAGGLTLARQLDDTRYSVRVASDRELDIAAPAAHA
                     YRLTAPGSTQITLLVEFSTGAPPAQMPAAETAREAVAKGWEKFWKHGGVVDFTGSRDP
                     RAIELERRVVLSQYLTAVNSAGDMPPQEEGLFSNSWNGKFHLEMHAWHSAHFAVWGRP
                     ELLERSMPFYFNQLPMAKARAKAHKVRGAWWPKMTGPEGRESPSKVNPFIMWQQPHPI
                     YLAEVLYKAKPTRGTLEKYREIVFETAELLASWPFYDRKAERFVLGPPLIPAQENFEP
                     QETWNPTFELEYWRFGLATAQEWRKRLGLAKDPKWDNVLFRLSKLPEKDGVYLAAESQ
                     QDLWERARSPECSNGHTKPECPNRDHPSFVAALGLLPGWGADRETMRRTLAAVLKDWD
                     LRQTWGWDWPMLAMTATRLDEPEKAVDFLLWDARNFNFGKSGMTPRVHLDQNVAELLG
                     NAPPESPDGPGYRRAAETYFPSNGSLLLAVGLMVSGGSDVHELNPGFPPNGQWIVHSE
                     GILPLP"
     gene            3758..4729
                     /locus_tag="VIV63_13065"
     CDS             3758..4729
                     /locus_tag="VIV63_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6125576.1"
                     /translation="MTLTELRYIVALAQEQHFGRAAERCHVSQPTLSIAVKKLEEELG
                     VLFERGKQKILPTPLGEKIVGMASRVLEQAAAIKDVADADKDQLEGPIALGTLPTIGP
                     YLLPQFIPLLQETTSKLSLYVEEGNIGELASKLRSGDLDAILVTAPFVEADVVTQPLF
                     DEPFVVLLPADHRLASKVMVSGQDLLPSELLLLGEGDSMREQILAAFPHLDPNRETGA
                     GVRTFTQGATLETLRHMVASRLGVTILPQTAAEAPLYAPNLLVTRPFENPAPKRSLAL
                     AWRVSFPRHKAIDLLRRAIQASSSAYWNYNTARAQEAPGVMVENRNW"
     gene            4729..6213
                     /locus_tag="VIV63_13070"
     CDS             4729..6213
                     /locus_tag="VIV63_13070"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6125577.1"
                     /translation="MRPLSKIVIVGGGTSGWLAASMLCQHLKRELCEIELVESEELGT
                     IGVGESTVPPFVGLIQRLGIDESEFVRATDATYKLGIKFVGWHQRSDSYFHPFGVIGK
                     PIGNHDFYQCWLKARAEGNTDALQDFSPCNVMAEQGRFFHPAKARNTPIGGANYALHV
                     DALLVTKFLRTYAEARGLKRTEGKVVEVGQRPDGFIESVTLASGRKIEGDFFIDCTGF
                     KSLLIGKTLGVENIDWSDYLPCDRAVVCKTENKGALVPYTRATAQPAGWSWRIPLKQR
                     VGNGYVYSSRFASDATAKATLLRSLDAACIEEPRIIPYTTGHRRQFWKHNCLSLGLAS
                     GFIEPLEATSIHLIARGMDFFLRYFPDKDCDAALIREYNRRMVADFEEVRDFIVLHYA
                     ATARDDTPFWQWCKNIPLPDTLRERIELFKAHGAMREGVDELFRASSWQSVFEGMGIR
                     PHGHCPRVENLDYAQIEQTLRNAKAAIAGMVEHLPTHEQFLRTA"
     gene            6251..>6487
                     /locus_tag="VIV63_13075"
     CDS             6251..>6487
                     /locus_tag="VIV63_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125578.1"
                     /translation="MAVLAMLAGSLPAAAQPQHVPEVEFFSTAFPELLSRLRTLVNTA
                     EYCNAAPAAACRASFVLPEPIVDDMRYFLDRITTF"
BASE COUNT         1143 a         2081 c         2085 g         1178 t
ORIGIN      
        1 tagacaggcg aggacaggcc gctgaccagc tcgtctatct tcaccgagtt cccggtcttg
       61 aagcactggt tggccgccgc gccgatcagg atcgaataca ggccgccgcg ttcgtccgcc
      121 gcgcgcttgt acttgtcggg ctcggccgtg ccgaacagtt ccgtcagcat gacgttgtcg
      181 ccgccaccgt ggccgccggt gccggtccag acctcgaggt cctgcgcctt gccacgcagc
      241 gggatgatgc gcgtgcgttc gcggtcgccg cccgtgccct ggtagttagt ggcgccggcg
      301 gagatggcgc cgccctcgac gatctcgtgc tcgatacggc ccttggtgcc gttgaaggcg
      361 atggtgtagc cctcccacga attgcaggcg ttgagcgagt aggacaacgt ggcgcccatc
      421 ttgtacttca cgatgacgtt catcgtgtcc tcgatgttga tgtccgggcg gaacacgcag
      481 cgatcgcgca gatagccgtc gtactgctcg ttgtcgagat acagcgactt gaagttcttg
      541 tcggccgcga ggtcgagata gaacgtgcac ttctctttct cggggcaggt gcggcagcgc
      601 tcgtggtggc tcgtgagccc catgcgcttc gccatcgccg gcgtgtagaa ctctttcttg
      661 ccgtacgcca tgacgatctc gggatcgctg cccagccacc agttcacgag gtcgaagtga
      721 tgcgtggcct tgtggaccat gagaccgccg gagatttcct tctggctgtg ccagcggcgg
      781 aaatagtcgg cgccatgcga cgtgttgagc agccagtgga agtcgacgga gagcacgtcg
      841 ccgatggcgt tgctcatgag caggtccttg acctgcgtgc gcggcggcga atagcggtag
      901 ttgaacaaca cgcgcacgtg gcggttgttg cgcttgcagg cgtcgaggat gcgctgcgcc
      961 ttctcgggcg tggtagtcag cggcttctcc gtgatgcagt cgcagccggc gtcgagcgcg
     1021 cgcacgatgt agtcgtcgtg gaatgcatca gggcaggtga cgacgacgca gtcgggcttc
     1081 aggtccttca gcatcttgtc gaaatcggtt gccgcgtact tcttcggctt cgatccgttc
     1141 ggcgcgacga acgcggccgc gacatcgaga cgtcccgcgt tcgtgtcgca gatgccgacg
     1201 agctcattgc cctcggcaaa cgtcttggtg atggcggtga ggtacatgcg cgaacgcgag
     1261 cccacgccga cgttcacgta ttttcggcgg cgtccggccg ctagcgagct cgcagcgaat
     1321 cccacggcgg tgacgcctgc ggttttcaga agggcacgcc ggtcaatcgt cgactcactc
     1381 atttatatgt cccccgcttt gttcgtgacg tacgatccgc cgcgaagata cctcaaattc
     1441 gatcaactgc gctcatgtgc gcgcacgccg gatatcgatt ttcagcacat acttcgccgc
     1501 catgtactta cgcgcgctcg ttttcctgac tacgacttta gtggtgtcgc agcttgcggt
     1561 tgccgcgccc atcgaccgtc acgcgctggt gacccgccac aaccccacca tcaccaccgt
     1621 ggatagggcg gcgccgttca tggtgggcaa cggcaatctc gcgttcacgg cggacatcac
     1681 cgggctgcag acctttccgg agcaatactc cgcgctcgtg ccgctgatga cccaggcgca
     1741 gtgggcgtgg cacagctttc ccaatccgca gaagttctcg ctcgcgcagg ccgaagtgcc
     1801 gatcgacgtg cgcggcaaga aacagaagta cccctacctc accgactggg agcaggcgaa
     1861 gagacccgag atccagtggc tgcgggaaaa tccgcatcgc ttctcgctgg cgcgcctcgg
     1921 cctgcacctg gtgaacgccg atgggaagat cgccgcattc ggcgagctca cggctacgcg
     1981 tcagaccctc gacatgtgga ccgggcggct cgtgagcagc ttcgagttca atggcacgcc
     2041 cgtcgaagtg gaaacctcgg ttcatccgga tcgcgacctg ctcatcgtgc gcttgcgctc
     2101 cacgctgctc gccgatggac gtctgggatt caacctcagg tttcccggag tcggcgcgaa
     2161 gttgaatccg gaccctgccg attggacacg ccccgaagcg catacgacgc aggaggtcac
     2221 gcgcaacgcg ggcggcctca cactcgcgcg ccagctcgac gacacgcgct actcggtgcg
     2281 ggtcgcctcg gatcgcgagc tcgatatcgc cgcgcccgca gcacatgcgt accggcttac
     2341 cgccccgggc tccacgcaga tcaccttgtt ggtggagttc tcgacgggtg cgccgccggc
     2401 gcagatgccg gccgccgaga ccgcccgtga agccgtggcg aaaggctggg aaaaattctg
     2461 gaagcacggc ggcgtggtgg acttcaccgg cagtcgcgac ccgcgcgcga tagaacttga
     2521 gcgtcgcgtc gtcctgtcgc agtacctcac ggccgtcaac agcgcgggcg acatgccgcc
     2581 tcaggaggaa gggctcttct ccaatagctg gaacggcaaa ttccatctcg agatgcatgc
     2641 ctggcattcg gctcatttcg cggtgtgggg ccggcccgag ctgctcgagc ggagcatgcc
     2701 gttctatttc aaccagctgc cgatggcgaa ggcgcgtgcg aaggcgcaca aggtgcgcgg
     2761 cgcctggtgg ccgaagatga ccggccccga agggcgcgag agcccgagca aggtgaaccc
     2821 gttcatcatg tggcagcagc cgcatcctat ctacctggcg gaagtgctct acaaggcgaa
     2881 gccgacgcgg gggacgctgg agaaataccg cgagatcgtg ttcgagaccg ccgagctgct
     2941 cgcgagctgg ccgttctatg accgcaaggc cgaacgcttc gtgctcggcc cgccgctgat
     3001 tcccgcgcag gagaacttcg agccgcagga gacgtggaat ccgacgttcg aactcgagta
     3061 ctggcggttc gggctcgcga ccgcgcagga gtggcgcaag cgcctgggat tggccaagga
     3121 tcccaagtgg gacaacgtgc tgttccggct ctcgaagctg ccggagaaag acggcgtcta
     3181 tctggccgcc gaatcgcagc aggatctgtg ggagcgcgcc cgctcgccgg aatgctccaa
     3241 cggacacacg aagccggaat gtccgaaccg cgatcacccg tcattcgtgg ccgcgctcgg
     3301 tttgctgccg ggctggggcg cggaccgcga gaccatgcgc cgcacgctcg ctgccgtgtt
     3361 gaaagactgg gatctgcgcc agacctgggg ctgggactgg ccgatgctgg cgatgaccgc
     3421 cacgcgtctc gatgaacccg agaaggccgt ggatttcctg ctgtgggatg ccaggaactt
     3481 caacttcggg aagtccggca tgacgccccg cgtgcacctc gaccagaacg tggcggaact
     3541 cctgggcaac gcgccgcccg agtcgcccga tggtcccgga tatcggcgcg ccgcggaaac
     3601 ctacttcccc tcgaacgggt cgttgttgtt agccgtgggc ctcatggtct cgggcggcag
     3661 tgacgtccat gaactgaatc cgggctttcc gccgaacggg cagtggatag tccatagcga
     3721 aggcatactt cccttgccct gacgggatga aggtgtcatg actctcaccg aactccgtta
     3781 catcgtcgcg cttgcccagg agcagcattt cggccgcgcc gccgaacgct gccacgtgag
     3841 ccagcccacg ctgagcatcg cggtgaagaa gctcgaagaa gaactcggcg tgctgttcga
     3901 gcgcggcaag cagaagatcc tgccgacacc gctcggcgag aagatcgtgg gcatggcatc
     3961 gcgggtgctg gagcaggccg cggccatcaa ggacgtcgcc gacgccgaca aggaccagct
     4021 cgaaggcccg atcgcgctcg gcacgctgcc gacgatcggt ccctatctac tgccgcagtt
     4081 catcccgctg ctgcaggaga ccaccagcaa gctgtcgctg tacgtcgagg aaggcaacat
     4141 cggcgaactc gcgagcaagc tgcgcagtgg cgacctggac gccattctcg tgaccgcgcc
     4201 gttcgtcgag gcggacgtgg tgacacaacc cctgttcgac gaacccttcg tcgtgttgct
     4261 gccggcggat caccggctgg cgtcgaaagt catggtttcc ggccaggacc tcctgccctc
     4321 ggagctgctg ttgttaggcg agggcgacag catgcgcgag cagatcctgg cggcgtttcc
     4381 gcacctggac ccgaatcgcg aaacgggcgc cggcgtgcgt acttttacgc agggcgccac
     4441 gctcgaaacc ctgcggcata tggtcgcctc gcgcctcggg gtcaccatcc ttccgcagac
     4501 ggccgccgaa gcgccgctgt atgcgccgaa tctgctggtc acccgcccgt tcgagaatcc
     4561 ggcccccaag cgaagcctgg cgctggcctg gcgtgtgagt tttccgcgac ataaggccat
     4621 cgatctgctg cgccgcgcga ttcaggcgag cagttccgcc tactggaatt acaatacggc
     4681 gcgtgcccag gaggcccctg gggtgatggt ggaaaaccgg aactggtgat gcggcccctt
     4741 tccaaaatcg tgatcgtcgg cggcggcacc tccgggtggc tcgccgcctc catgttgtgc
     4801 cagcacctga agcgcgagct ctgcgaaatc gagctcgtgg aatcggagga gctgggcacc
     4861 atcggtgtgg gcgaatccac cgtgccgccg ttcgtcgggc tcatccagcg cctcggcatc
     4921 gatgaatccg aattcgtgcg cgccaccgat gccacctaca agttggggat caagttcgtc
     4981 ggctggcacc agcgcagcga ttcgtacttc catcctttcg gcgtcatcgg caagccgatc
     5041 ggcaatcacg atttctacca gtgctggctc aaggcgcgcg ccgaaggcaa caccgacgcg
     5101 ctgcaggatt tctcgccgtg caacgtcatg gcggagcagg gccgtttctt ccatccggcg
     5161 aaggcgcgca acacaccgat aggcggcgcc aactacgcgc tgcacgtcga tgcgttgctg
     5221 gtcacgaagt tcctgcgcac gtacgccgag gcgcgcgggc tcaaacgcac cgaaggcaaa
     5281 gtggtcgagg tcgggcagcg gcccgatggt ttcatcgaaa gcgtcacgct cgccagcggt
     5341 cggaagatcg aaggcgactt cttcatcgac tgcaccggct tcaagagcct gctgatcggc
     5401 aagacgctgg gcgtcgagaa catcgactgg tccgactatc taccctgcga tcgcgccgtg
     5461 gtctgcaaga ccgaaaacaa gggagcgctg gtcccgtaca cgcgcgccac cgcgcagccg
     5521 gcgggctgga gctggcgaat cccgttgaag cagcgagtcg gaaacggcta cgtctattcc
     5581 agccgtttcg cgagcgacgc cacggcgaag gccacgttgt tgcgctcgct cgatgccgcc
     5641 tgcatcgaag agccgcgaat catcccgtac accaccgggc atcgccgcca gttctggaag
     5701 cacaactgcc tgtcgctggg cctggcgtcg ggattcatcg agccgctcga ggcgacgtcc
     5761 atccacctga tcgcgcgcgg catggacttc ttcctgcgtt acttccccga caaggattgc
     5821 gacgcggcgc tgatccgcga atacaaccgg cgcatggtcg cggacttcga ggaagtgcgc
     5881 gatttcatcg tgctgcatta cgcggccacc gcgcgcgatg acacgccgtt ctggcagtgg
     5941 tgcaagaaca ttccgttgcc ggacacgctg cgtgaacgca tcgagctgtt caaggcgcac
     6001 ggcgccatgc gcgaaggcgt cgacgaactg ttccgcgcct cgagctggca gtcggtgttc
     6061 gaaggcatgg gtatccgtcc gcatgggcat tgcccgcgcg tcgagaatct cgattacgcg
     6121 cagatcgagc agacgctgcg caatgccaag gccgccatcg ccggcatggt cgagcacctg
     6181 ccgacgcacg agcagttcct gcgcactgcc tgacaaggtg acaaccgtgc tttcgcggcg
     6241 aggctggcgg gtggcagtgc tcgcgatgct tgcaggttcg ctgccggcgg cggcgcagcc
     6301 tcaacacgta cccgaggttg aattcttttc gacggcattt ccggagctgt tgtcccgcct
     6361 ccgaacgctg gtcaacaccg cggagtattg caatgccgct cccgcggcgg cctgccgggc
     6421 gagtttcgtg ctgccggaac cgatcgtgga cgacatgcgt tatttcctcg atcgcatcac
     6481 tactttt
//
LOCUS       DATDEU010000548         2340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_981852, whole genome shotgun sequence.
ACCESSION   DATDEU010000548 DATDEU010000000
VERSION     DATDEU010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981852"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..822)
                     /locus_tag="VIV63_13080"
     CDS             complement(304..822)
                     /locus_tag="VIV63_13080"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEY6125579.1"
                     /translation="MRPLRILAVAVTLAVANCLPGALRAQEQQPSPESMAAAQALFAL
                     LFTHGFATLNAQAVEAAWPGIESALRTQRPNIDPATLADLRREFERIRLARLSEVVKD
                     IPAIYARHLNAEDMRVLAAFYGTATGTKMLQAMPKVLPEAFASVLPRMQPMMAETQDS
                     FLKLLRERGLLN"
     gene            complement(901..1863)
                     /locus_tag="VIV63_13085"
     CDS             complement(901..1863)
                     /locus_tag="VIV63_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY6125580.1"
                     /translation="MSSATPAPGLIPAAPDVAREMAQWLSYLAAERRMSPKTVEAYER
                     DLRQFLGFLSGHLDGRVTLSALARLAPLDVRAFLAARRADGIGSRSLMRMLAGVRSFA
                     RFLERNGKGKVGALAAVRTPKTGKTLPKPLAIAVAKRMADTDLRAGEERDPWILARDA
                     AVLALLYGSGLRISEALGLKRGDIPAPGRGDVITVTGKGNKARMVPVLAKVAELAADY
                     IALCPHQLPTDGPLFVGAKGGPLSPRIIQLAMARLRGALGLPESATPHALRHSFATHL
                     LARGGDLRAIQELLGHASLSTTQIYTAVDTERLLEVYRSAHPRA"
     gene            1930..>2340
                     /locus_tag="VIV63_13090"
     CDS             1930..>2340
                     /locus_tag="VIV63_13090"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125581.1"
                     /translation="MARRVVDVLLPVPLDQAYSYRVPDELMLAPGDLVSVQLGARVAT
                     GVVWAENANPNPRLDNRIKDVEEKLDLPPLKPELRTFIDWVSGYTLTARGTVRRMALR
                     MGEHLGPARERVGVRLAGSAPKRMTVARDRVLAFL"
BASE COUNT          375 a          763 c          799 g          403 t
ORIGIN      
        1 aaaagcgcaa tcaccgcgat cagcagcgcg atcttcttgt tgaagctgtc cgccagtagt
       61 cttttcccgc cgccgccacc atgcccgtgc tcctcatgct cgatgtgatg ctcgacctca
      121 tgctggaccg cgtgcacgcc atgactcatg tcgccctccg tgcttcgttc gacgacgatg
      181 tatcgaactg gttcgcttgt gcaaggggga aacagacgag gcagcgttcg ccgtgtctgc
      241 ctcgcgccgg cagtcaggta aattatcaaa gcgcacgccg aagttggcgc gacggcttcg
      301 ccgtcagttg agcaggccgc gctcgcgcag gagcttgagg aaggaatcct gcgtctccgc
      361 catcatgggc tgcatgcgcg gcagcacgct ggcgaatgct tccggcagga cctttggcat
      421 cgcctgcaac atcttcgttc cggtcgcggt gccatagaag gcggccagta cgcgcatgtc
      481 ctcggcgttg agatggcgcg catagatggc cggaatatcc ttcacgacct cggacagccg
      541 cgcgaggcgg atgcgctcga attcccggcg caggtcggcg agcgttgctg ggtcgatatt
      601 cggccgttgc gtgcgcaagg cgctttcaat gccgggccag gcggcctcca cggcctgcgc
      661 gttgagggtg gcgaagccgt gggtgaacag cagcgcgaac agggcctgtg ccgccgccat
      721 gctttcgggt gaaggttgtt gctcctgcgc gcgcagcgcg ccaggcagac aatttgccac
      781 cgcgagcgtg acggcaacag caagaatgcg cagtggccgc atgtggcctc tccggaacga
      841 agccgggaag ctagcatgtc atcgccgtcc tgcaacgggt gtgcgcgcaa ttgcgagcgg
      901 tcaggcccgc ggatgggcgc tgcgatagac ttccagcagc cgttcggtat cgacggcggt
      961 gtagatctgt gtcgttgaaa gcgaggcgtg cccgagcagt tcctggatcg cacgcaagtc
     1021 accgccgcgc gccagcaggt gcgttgcgaa ggaatggcgc agcgcgtgcg gtgtagcgct
     1081 ttcgggcagg ccgagcgctc cgcgcagccg cgccatcgcc agctggatga tgcgcggcga
     1141 gagcgggccg cctttggcgc ccacgaacaa gggaccgtcg gtcggaagct gatgcgggca
     1201 gagcgcgatg tagtccgccg cgagctcggc gaccttggcc agcaccggca ccatgcgcgc
     1261 cttgttgccc ttcccggtga cggtgatgac gtcgccccgt ccgggtgcgg gaatgtcgcc
     1321 gcgcttgagg cccagcgcct cggagatgcg caggcccgag ccgtagagca atgcgagcac
     1381 cgcggcatcg cgggcgagaa tccagggatc gcgctcctcg ccggcgcgca aatcggtgtc
     1441 ggccatgcgc ttggcgaccg cgatcgcgag cggcttcggc agtgtcttcc cggtcttggg
     1501 cgtgcgcacg gcggcaagcg cgcccacctt gcccttgccg ttgcgctcga ggaagcgcgc
     1561 gaaggaacgc acgcccgcca gcatgcgcat cagcgaacgg ctgccgatgc cgtcggcgcg
     1621 tcgtgcggcg agaaaggcgc gcacgtcgag cggcgcaagg cgcgccagcg ccgacagcgt
     1681 cactctgccg tcgagatggc cggacaggaa gccgaggaac tggcgcagat cgcgctcgta
     1741 ggcctccacc gttttcggcg acatccgccg ttcggctgcc agatatgaga gccattgcgc
     1801 catttcgcgc gcgacatcgg gggccgctgg aatgaggccg ggagcgggtg ttgcgcttga
     1861 catggcacta tatatctctc cccttccttc acgcattctt aaatcgcttg caaccggccc
     1921 gattgcccta tggccagacg cgtcgtcgat gtcctgctcc cggtcccgct cgatcaggcc
     1981 tattcctacc gggtgccgga cgagctcatg ctggcgcccg gcgacctcgt gagcgtccag
     2041 ctcggggcgc gtgtggcgac cggagttgtc tgggcggaga acgccaatcc caatccccgt
     2101 ctcgacaacc gcatcaagga cgtcgaggag aagctcgatc tgccgccgct aaagccggag
     2161 ctgcgcacgt tcatcgactg ggtctccggc tacacgctta ccgcccgcgg cacggtgcgg
     2221 cgcatggcat tgcgcatggg cgagcatctg ggtcccgcgc gtgagcgcgt cggcgtgcgc
     2281 ctcgccggtt ccgcgccgaa gcggatgacg gtcgcgcgcg accgcgtgct cgcgttcctt
//
LOCUS       DATDEU010000549         3382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2148717, whole genome shotgun sequence.
ACCESSION   DATDEU010000549 DATDEU010000000
VERSION     DATDEU010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148717"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1593
                     /locus_tag="VIV63_13095"
     CDS             295..1593
                     /locus_tag="VIV63_13095"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY6125582.1"
                     /translation="MLVGSNVRRVAVVGGMRIPFARGHGAYSRVGNQDMYTAVLQALV
                     AKYRLAGERLGDVTGGAVLKHSKDFNLVRECVLSSGLDPQTPGLDMQRACGTGLDAAI
                     SVAMKIALGQMDSGIAGGFDTVSDPPIVYSREYQQLLLRAFRGRSAWGRIKPFFGLRP
                     RHFKPVMPGVLEPRTGLSMGEHCEQMAKTWKISRADQDALANLSHVNAGKAWARGFFS
                     DLVIGEYQGLKIDNNVRADSTLEKLGSLKPAFDRRSGQGTLTAGNSTPLTDGASAVLL
                     ASEEWARARNLPVLAYFSFSKQWAVDYVGGNEGLLMAPAYAVPAMLKDANLTLQDFDF
                     YEIHEAFAAQVLCTLKAWESPEYCKEKLGLDQPLGSIDRAKLNVNGSSVAIGHPFAAT
                     GTRIVASLAKQLAESPTAKRGLISVCTAGGMGVVAILEKA"
     gene            1676..2803
                     /locus_tag="VIV63_13100"
     CDS             1676..2803
                     /locus_tag="VIV63_13100"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY6125583.1"
                     /translation="MKRYVHFPRVEGQASRQAHADLPAGTYEREISKEGFFGPAAHLY
                     HRHPPTGWVDFEGPLRPHAFDCTKLTGAQASPWNAAALLHNASVRLRYWSLAANMPQL
                     VRNADGDDLLFVHAGSGDLFCDYGHLAIEAGDYVLIPRGTLWRVECAQPLHMLLIEAT
                     NNSYRLPDKGLVGPHAIFDAAMLDTPRIDDAFKDQQEDKEWRVAIKRRGAVSTVTYPF
                     NPLDAVGWHGDLSVVRINVRDLRPLMSHRYHLPPSAHTTFVADRFVVCTFVPRPFETD
                     PGAMKVPFFHNNDDYDEVLFYHAGDFFSRDNIHPGMITLHPGGFTHGPHPKALKNVHQ
                     QTKPATNEYAVMIDTRDALEIAPAAAQVEWGGYVDSWKGAT"
     gene            2800..>3382
                     /locus_tag="VIV63_13105"
     CDS             2800..>3382
                     /locus_tag="VIV63_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6125584.1"
                     /translation="MKLATLKRGGRDGRLVIVNKALTYCQLVPGIAPTLQAALDDWAS
                     VSPKLADRAHALELNHSNSDVMPFDVAQCAAPLPRAYHWVDGSAYVNHVELVRKARGA
                     DMPASFWTDPLVYQGGSDDLLGPRDDAPFGDTAWGIDLEAEVAVITDDVPMGTSPADA
                     AAHIKLVVLVNDWSLRGLIPGELAKGFGFYQSKP"
BASE COUNT          602 a         1129 c         1061 g          590 t
ORIGIN      
        1 acaccgagag cgaacgcgta gatgagcgtg cggtatcggg gattctcggg ataaaccttg
       61 gtcttgtcgc cccaccacgc aatggcgaag aggccggcca tgtagaccag cgacgccagc
      121 agcaagaccc agcctgaaac ggtcgtcgtc atttagttga gtagacgata gtccaatttt
      181 tgcgcgcgtg ttcccgaacg tattctcgac ctccctgctc caatagagcg aaggcgtttt
      241 tattggtgac gtcgacgtgg catcctagcg cgcgcttttc gccgggagct ctccatgctg
      301 gtcggatcca acgttcgtcg tgtcgccgtg gtcggtggca tgcgcattcc cttcgcgcgc
      361 gggcacggcg cttattctcg cgtcggcaac caggacatgt acaccgcggt gctgcaggcg
      421 ctggtcgcga aataccggct ggccggcgag cggctcggcg atgtcacggg cggcgccgtg
      481 ctcaaacact ccaaggactt caacctcgtg cgcgagtgcg tgttgtcgtc gggactcgat
      541 ccgcagaccc cgggcctcga catgcagcgc gcctgcggaa cggggctcga cgccgcgatc
      601 tcggtggcca tgaagatcgc gctcgggcag atggactccg gcatcgccgg cggcttcgac
      661 acggtcagcg atccgcccat cgtctattcg cgtgaatacc agcagctgct gctgcgcgcg
      721 tttcgcggac gcagcgcgtg gggacgcatc aagccgttct ttggcctgcg tccgcgtcac
      781 ttcaaacccg tgatgcccgg cgtgctcgag ccgcgcacgg gtctgtcgat gggcgaacac
      841 tgcgagcaga tggcgaagac gtggaagatc tcgcgcgccg atcaggatgc actggcgaac
      901 ctgagtcacg tcaacgccgg caaggcgtgg gcccgcggtt tcttcagcga cctggtgatc
      961 ggcgaatacc agggcctcaa gatcgacaac aacgtgcgcg ccgacagcac gctcgagaaa
     1021 ctcggctcgc tcaagcccgc gttcgaccgg cgcagtggcc agggcacgct caccgcggga
     1081 aattccacgc cgctcaccga cggcgcgtcg gcggtgttgt tagccagcga agaatgggcg
     1141 cgcgcgcgga atcttccggt gctcgcgtat ttcagcttca gcaagcagtg ggccgtggac
     1201 tatgtcggcg gcaacgaagg actgctcatg gcgcccgcgt acgcggtgcc cgccatgctc
     1261 aaggatgcga atctcacgct gcaggacttc gacttctacg agatccacga ggcattcgcg
     1321 gcgcaggtgc tgtgcacgct caaggcgtgg gaatcgcctg agtactgcaa ggagaagctg
     1381 ggactcgacc agccgctcgg cagcatcgac cgcgcgaagc tcaatgtgaa cggttcctcg
     1441 gtggccatcg gccacccgtt cgcggccacc ggcacgcgca tcgtcgcgtc gctggcgaag
     1501 cagctggccg aatcgccgac ggcgaagcgc ggactcatct ccgtctgcac ggcgggcgga
     1561 atgggtgtag tcgcaatcct cgagaaggcg tgacggcagg gcatcgggcc agtcccattc
     1621 ttgtaaatgg tttcaaatgt aactaaattg gccgcccgac tgcgcgaggc ggcccatgaa
     1681 gcgctacgtc cattttccca gggttgaagg ccaggcttcg cgccaggcac acgccgacct
     1741 gcccgcgggc acgtacgaac gcgagatcag caaggaaggc ttcttcggac ccgcggccca
     1801 tctctatcac cgccatccgc ctacgggctg ggtcgacttc gaaggcccgc ttcgccccca
     1861 cgcgttcgac tgcacgaaac tcaccggcgc ccaggcttcc ccgtggaacg ccgcggcgct
     1921 gctgcacaac gcctcggtgc ggttgcgcta ctggagcctc gccgcaaaca tgccgcagct
     1981 cgtgcgcaat gccgatggcg acgatctcct gttcgtgcac gccggcagtg gcgacctgtt
     2041 ctgcgactac ggccatctcg cgatcgaggc cggcgactac gtcctcattc cgcgcggcac
     2101 cctgtggcgc gtggagtgcg cgcagccgtt gcacatgctg ctcatcgagg ccactaacaa
     2161 ctcgtaccgc ctgcccgaca agggactcgt cggtccgcac gccatcttcg acgcggccat
     2221 gctcgacacg ccgcgcatcg acgatgcctt caaggaccag caggaagaca aggaatggcg
     2281 agtcgccatc aagcggcgtg gcgctgtttc cacggtcacg tacccgttca acccgctgga
     2341 tgcggtgggc tggcacggcg atttgtcggt ggtgcgcatc aacgttcgcg acctgcggcc
     2401 tctcatgagc caccgctatc acctgccgcc gtcggcccac accactttcg tcgccgaccg
     2461 cttcgtggtc tgcacattcg ttccgcggcc attcgaaacc gatccgggcg cgatgaaggt
     2521 tccgttcttc cacaacaacg acgactacga cgaagtcctt ttctatcacg ccggagattt
     2581 cttcagccgc gacaacatcc atcccggcat gatcacgttg catcccggcg gtttcacgca
     2641 cggtccgcat ccgaaggcgc tgaagaacgt gcaccagcaa accaaacccg ccaccaacga
     2701 atacgcggtg atgatcgaca cgcgcgatgc gctggagatt gcgcccgcag ccgcgcaggt
     2761 ggaatggggt ggatacgtcg atagctggaa aggcgccacg tgaagctggc gaccctcaag
     2821 cgcggcggtc gcgacggccg gctcgtcatc gtcaacaagg cgctgacgta ttgccagctc
     2881 gtgcccggca tcgcgcctac acttcaggcg gcgctcgacg actgggcgtc ggtgtcgccg
     2941 aagctcgccg atcgcgccca cgcgctcgag ctcaaccatt cgaactccga cgtgatgcct
     3001 ttcgatgtcg cccagtgcgc cgcgccgctg ccgcgcgcgt accactgggt ggacggcagc
     3061 gcgtacgtga accacgtcga actcgtgcgc aaggcgcgcg gcgccgacat gccggcgtcc
     3121 ttctggaccg acccgctggt ctaccagggc ggctccgacg acttgttagg cccgcgcgat
     3181 gacgcccctt tcggcgacac ggcctggggc atcgatctcg aggccgaagt cgcggtgatc
     3241 accgatgacg ttcccatggg caccagcccc gccgatgccg cggctcacat caagctggtg
     3301 gtgctggtga acgactggtc actgcgcggc ctcatcccgg gcgagctcgc gaagggtttc
     3361 ggcttctatc aatcgaagcc ca
//
LOCUS       DATDEU010000550         2786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2149164, whole genome shotgun sequence.
ACCESSION   DATDEU010000550 DATDEU010000000
VERSION     DATDEU010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149164"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VIV63_13110"
     CDS             complement(<1..441)
                     /locus_tag="VIV63_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6125585.1"
                     /translation="MSAKQILVVEDERPIREMIAFGLRRAGFEVREAADAQSGRAEVA
                     NKRPDLLLVDWMLPDTSGLEFTRALKRDRETRELPVIMLTARAEEGDKVAGLEGGADD
                     YITKPFSPRELLARINAVLRRTTPVEGTERIDVEGLVLDQSSQRV"
     gene            complement(514..1191)
                     /gene="tsaB"
                     /locus_tag="VIV63_13115"
     CDS             complement(514..1191)
                     /gene="tsaB"
                     /locus_tag="VIV63_13115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY6125586.1"
                     /translation="MKILALDTATEACSVSLCIGDRSIDRYVELERGHAEQLLPMVDS
                     VLAEGGIALGLLDAIAFGRGPGGFTGVRLAASVAQGLAFGAQVGVVPVSDLAAVAQRV
                     VQINPAVRRVLVANDARMREVYWAAFEFQGALESGAEHVSASAAVQLPGGAGVSWAAA
                     GRGLTAWPELADRCRAAGAELHPELLPRASEVLTLARIAVAARQILDPADALPVYVRD
                     RVTQTSL"
     gene            complement(1188..>2786)
                     /locus_tag="VIV63_13120"
     CDS             complement(1188..>2786)
                     /locus_tag="VIV63_13120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY6125587.1"
                     /translation="LAGTRDRTLSRVERWAGTTRTGDLAEVPDLGDAHPLWPQITSTR
                     ENCLGARCADIGKCHVAEARRKAVEADIVIVNHHLLLADLALKEDGFGDLLGAADAVI
                     LDEAHQIPDLATQFFGTRLGSRQVEMLLRDARQELGLARAPATTLASEVGGVEKALAA
                     LAEVFRLTPRPDWLAADTPMADAAEDLSRALRELTGALNEYSREPGFAQCSARSTELA
                     TRLEAVMHAEEHEGARSVELTQRGFTLSLLPFDIAARFSQIAGGTKAAWVFTSATLSV
                     GEDFAHFTSRLGIGDAETLAIPSPFDFETQALLYLPPQLPDPASGTHTGAVVDVSVPL
                     IEASAGGAFVLFTSHRALQRAAQLLRDRWAELSDYPLLVQGEAPREQLLRQFRESGNS
                     VLLGTSSFWEGVDVKGDALRLVIIEKLPFSSPDDALTKARIEHLKATGGNAFREFQLP
                     EAALTLKQGVGRLIRSETDRGVVVICDPRLVDKPYGRVFRASLPPMPVTRVSADAERF
                     LRRIAGTVAAPAELTRVDIDSEIA"
BASE COUNT          457 a          932 c          948 g          449 t
ORIGIN      
        1 cacgcgctga ctgctctggt cgagcacgag cccttcgacg tcgatgcgct cggtaccttc
       61 caccggcgtg gtgcgtctca gcaccgcgtt gatccgggcg agcagttcgc gcggcgaaaa
      121 cggcttggtg atgtagtcgt cggcgccgcc ttcgaggccg gctaccttgt cgccctcttc
      181 ggcgcgcgcg gtgagcatga tgacgggcag ctcgcgggtt tcgcggtcgc gcttgagcgc
      241 gcgcgtgaac tcgaggccgc tggtatcggg aagcatccag tcgaccagca gcaggtcggg
      301 acgcttgttg gcgacttcgg cacggcccga ttgggcgtcg gccgcctcgc ggacttcgaa
      361 cccggcacgg cgcaagccga acgcgatcat ctcgcggatc ggccgctcgt cttcgactac
      421 cagaatctgt ttggctgaca tggttgctga cacggcatca caggtgacgt ttttgtggcg
      481 tgcgcattac aacggcagtc tattgcagat tgattacaaa gaggtttgcg ttacgcgatc
      541 tctgacataa acgggtaatg cgtccgccgg atcgaggatt tgccgcgccg ccaccgcgat
      601 ccgggcgagc gtgagcacct cgctggctcg cggcagaagc tccgggtgga gttcggcgcc
      661 cgccgcccgg catcggtccg cgagttcggg ccacgcggtg agaccccgcc ctgccgccgc
      721 ccaggacaca ccggcgccac cgggtaactg caccgctgcg gaggcgctca cgtgttccgc
      781 gcccgactcg agggcgccct ggaattcgaa ggccgcccaa taaacttcgc gcatgcgggc
      841 atcgttggcc acgagcacgc ggcgtaccgc gggattgatc tggacaactc gctgcgcaac
      901 agccgccagg tcggacaccg gcaccacgcc cacctgcgca ccgaaagcga gtccttgcgc
      961 gacactggcg gccaggcgca cgccggtgaa gccaccggga ccgcggccga atgcgatggc
     1021 gtcgagcagc cccagggcga tgccgccctc ggccagcacc gaatcgacca tgggcaacaa
     1081 ttgttcggcg tgaccccgct cgagttcgac atagcggtcg atcgaccggt caccgatgca
     1141 gagtgacacc gaacacgcct cggtggcggt gtcgagcgcg aggatcttca tgcgatctcg
     1201 ctgtcgatgt ccacgcgcgt gagttccgcg ggcgcggcca ccgtgcccgc gatgcgccgc
     1261 aggaatctct ccgcatcggc gctcacccgc gtgaccggca tgggcggaag gctggcgcga
     1321 aacacgcggc cgtaaggctt gtcgacgagc ctcgggtcgc agatgacgac cacgccgcga
     1381 tccgtctcgc tgcggatgag gcggcccaca ccctgcttca gcgtgagcgc cgcttcggga
     1441 agctgaaact cgcgaaacgc attgccgccg gtcgccttga gatgttcgat gcgcgccttg
     1501 gtcagggcat catcgggcga tgagaacggc agcttctcga tgatgaccag ccgaagcgcg
     1561 tcgcccttga cgtcgacgcc ttcccagaaa ctcgaagtgc ccagcaggac cgaattgccg
     1621 gattcacgga actgccgcag cagttgttcg cgcggcgctt cgccctgcac cagcagtgga
     1681 tagtccgaca gttcggccca gcgatcgcgc aacagctgcg cagcgcgctg caacgcacgg
     1741 tgactggtga acaacacgaa agcgccgccc gccgacgcct cgatgagcgg caccgagaca
     1801 tcgaccaccg cgccggtatg cgtgcccgac gcgggatcgg ggagctgcgg aggtagatag
     1861 agcaacgcct gggtttcgaa gtcgaacgga ctcgggatgg ccagcgtttc ggcgtcgccg
     1921 atcccgaggc gcgaggtgaa gtgcgcgaaa tcctcgccca ccgacagcgt ggccgacgtg
     1981 aacacccagg cggctttcgt gccgccggcg atctgcgaaa agcgtgccgc gatgtcgaac
     2041 ggcagcaggc tcagcgtgaa tccgcgctgc gtgagttcga cactgcgcgc gccttcgtgt
     2101 tcttcggcgt gcatcacggc ctcgagccgc gtggcgagtt cggtggaacg cgccgagcat
     2161 tgcgcaaatc ccggttcccg gctgtattcg ttcagcgcgc cggtgagttc gcgcagcgcg
     2221 cgagagagat cttcggcggc gtccgccatc ggtgtgtcgg cggccagcca gtccgggcgc
     2281 ggcgtcaagc ggaaaacttc cgcgagcgcc gccagcgctt tttcgactcc gcccacttcg
     2341 ctggcaagcg tcgtggcggg cgcacgcgcc agccccagtt cctgccgcgc gtcgcgcaac
     2401 agcatctcga cctgccggct gccgagccgc gtgccgaaga actgcgtggc gaggtccggg
     2461 atctggtgcg cttcgtcgag gatgacggca tccgcggcgc cgagcagatc gccgaacccg
     2521 tcttccttga gcgcgaggtc ggcgagcaac aagtggtgat tgacgatgac gatgtcggcc
     2581 tcgaccgcct tgcggcgagc ctcggcgacg tgacacttgc cgatgtcggc gcaacgcgcg
     2641 ccgagacagt tctcgcgcgt cgaggtgatt tgcggccaca gcgggtgggc gtcgcccagg
     2701 tccggcactt cggcgagatc gccggtgcgc gtggtcccgg cccagcgttc gacgcgcgac
     2761 agcgtgcggt cgcgcgtgcc ggccag
//
LOCUS       DATDEU010000551         6672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1711735, whole genome shotgun sequence.
ACCESSION   DATDEU010000551 DATDEU010000000
VERSION     DATDEU010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711735"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VIV63_13125"
     CDS             <1..241
                     /locus_tag="VIV63_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125588.1"
                     /translation="PAAPATPVATVKKRAAAKRRPAAKKKAKARARTVVRRKPKTAKR
                     SPAARKGVKKKAAKRAAVRKVARKARPVRRKSKRR"
     gene            complement(227..1300)
                     /gene="rnd"
                     /locus_tag="VIV63_13130"
     CDS             complement(227..1300)
                     /gene="rnd"
                     /locus_tag="VIV63_13130"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY6125589.1"
                     /translation="MIDTSDALTAFARQAASAPVLALDTEFMREKTYRAELCLVQIAT
                     GDGAACIDPLASPDLSVLTPLLGATGGVKIMHAARQDLEVLYPAVGMVQPVFDTQIAA
                     ALAGHPSQVGYGELVRRLLGVELAKAHTRADWSRRPLSAAEQEYALDDVRHLAALRAS
                     LLETLAAKGRVAWLDEELAGLANPDALRVAPEEAWKKIKGLPALDPQRHELAQALGAW
                     RERRAAERNRPRGWILDDVSLREIVLRLPRSTESLLALPEMQESVVRKCGEELLALVA
                     DARIGDPPPPLPRRERPDPAHLALVKRLADVATDVAKGLEINAEVLATRRELEKLAAG
                     KRDVSLLRGWRKDILGEKLLAAL"
     gene            1346..1897
                     /locus_tag="VIV63_13135"
     CDS             1346..1897
                     /locus_tag="VIV63_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125590.1"
                     /translation="MLVNLRALFGLLVDIVLLRRGPESLPASPSVLAIFVVLNGALSA
                     LIASLIPSHPDISIVELIVSPFVPMLWYLIAFSLAKKPERFVQTMVAFFGVNLLFQPV
                     VAPMLAALTPYMEKQDPAMPPPAALSLLFIVLSVWLLIVWVRIVRIAFEWPTIVAFVF
                     VLAQNLVAIFIYSAMFGVSSGKV"
     gene            1915..3285
                     /gene="mpl"
                     /locus_tag="VIV63_13140"
     CDS             1915..3285
                     /gene="mpl"
                     /locus_tag="VIV63_13140"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY6125591.1"
                     /translation="MHVHILGICGTFMGGIAAIAKAAGHRVTGSDKNVYPPMSTQLEA
                     LGIELISGFDANQLRPAPDVVVVGNVMTRGAPVIEALLESNIPYTSGPEWLAREVLRD
                     VHVLGVSGTHGKTTTSSMLAWILEHAGLNPGFLIGGVPGNFSVSARLGGRKFFVIEAD
                     EYDTAFFDKRAKFVHYRSRTLILNNLEFDHADIYENLAAIQKQFHHLVRTIPGSGRVL
                     WNAGDENLKATLAMGSWTPLEGFARTPGADVAWSLDVEEGGDYSKFVVLEGGKPAGTV
                     RWSLLGAHNAENALAAIGAARHAGVSVAQSIAALAEFKGVKRRMELRGEVRGISVYDD
                     FAHHPTAIASTIDGLRRKVGGKRIIAVLEPRSATMRMGVHRDTLAPSLSAADSVWMFA
                     PPDLGWDADAVVARLGPKGKIARSLDELVSGLAGNSASGDQVLIMSNGGFGGLHGKLL
                     EALARM"
     gene            3317..3877
                     /locus_tag="VIV63_13145"
     CDS             3317..3877
                     /locus_tag="VIV63_13145"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY6125592.1"
                     /translation="MFPLHTVLFPGGPLPLRIFETRYTDMVRRCMREQSPFGVVLIQD
                     GEEAGEVATTATVGCTARIADFHTLHDGLLGISCVGDRKFLVERVWRAADGLNMGEVS
                     WLPAERVVALPERYGHLATTVRRALEDLSEHYEYIEKKFDDAAWIGARLTELLPIELA
                     DKQALLELDDPIERLDTLLSVVPSPE"
     gene            complement(3880..4797)
                     /locus_tag="VIV63_13150"
     CDS             complement(3880..4797)
                     /locus_tag="VIV63_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY6125593.1"
                     /translation="MIFPKAMSRGLARGLAGVLLLAIGVQTAHARGETSVWSMKGTRN
                     TVYLAGSVHALPKGHSDFPEQLDRAYAAAQVIVLEVDLDDMNPLDAVKFITTNGTLPA
                     DQSLKEVVGEKSYQRISELAASLEVPEIVIAKLEPWAAALVLTQFALTKTGFDANLGI
                     DMQITERARADGKPIEGLETVIDQLSVFDDRSFEEQTRFLLDATEDAPRLNDDLHKLI
                     TAWRGGDLRALEKEFVKERAKSPELYDALLGTRNRQWLPKIEALLEQDRDYLVVVGAL
                     HFVGRDGLLELLRNHGHKAIAVPAAKKQN"
     gene            complement(4794..5561)
                     /gene="cpdA"
                     /locus_tag="VIV63_13155"
     CDS             complement(4794..5561)
                     /gene="cpdA"
                     /locus_tag="VIV63_13155"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF008359.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HEY6125594.1"
                     /translation="MSAIRVIQFTDPHLFGDIAGTLRGVNTFETLERTLADASGHIAM
                     SDAILVTGDLVQDDAGGYEHFRRLFARFGKPVLCIPGNHDFVPQMRAALAGRPFDMDG
                     PADIGAWRIVLLDSVVPDQAGGRISVEMLTKLDEDLGRARQRHAMVCLHHHPIAMNSR
                     WLDSVGLSNADEFWSVIDSHPQVKAVVWGHVHQNFEGARRGVRLFATPSTCAQFQPRS
                     DQFVIDNQPPAYRLLTLHSNGAIDTGVHSLTAAESAA"
     gene            5817..6509
                     /locus_tag="VIV63_13160"
     CDS             5817..6509
                     /locus_tag="VIV63_13160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6125595.1"
                     /translation="MKRPGKSDPSRPREDGYDYKREFYRSSSVAEDYDFHRFSTPERQ
                     KRNARKWAAIRAALRETTGVRTILDLPCGTGRFTGALAREGFEIVGSDISHEMLHKAA
                     SLDGLPQEIRGFVQANAEHLPLRNDSLDCVVSIRFMMHVDPVSRVRMLREFHRVSRRW
                     VIVDYRHCYSFRYLLTHTFGRIGIGRPPLSRVSRKELEKEFSDAGFAIRKVIRVSAPL
                     LSDKWIVLAERA"
BASE COUNT         1129 a         2159 c         2172 g         1212 t
ORIGIN      
        1 ccccgcggcg cccgccacgc cggttgccac cgtcaagaag cgcgctgccg ccaaacgccg
       61 gccggcggcg aagaaaaaag cgaaggctcg cgcgcgaacc gtggttcgcc gcaaaccgaa
      121 gactgcgaag cgcagtccgg ccgcccgcaa gggtgtgaag aagaaagccg cgaaacgcgc
      181 cgcggtgcgc aaggtcgcgc gcaaggcgcg gcccgtgcgc cgcaagtcaa agcgccgcta
      241 gcagcttctc gccgaggatg tccttgcgcc agccgcgcag caggctgacg tcgcgttttc
      301 cggcggcgag tttttcgagc tcgcggcgcg tcgccagcac ttcggcgttg atctccaggc
      361 ccttggcgac gtcggtcgca acgtcggcga gccgcttcac cagcgcgagg tgcgcgggat
      421 cgggacgttc gcggcgcggc aacggtggcg gcggatcgcc tatcctggcg tcggccacca
      481 gcgccagcag ttcttcgccg cacttgcgca ccacggactc ctgcatctcc ggcaacgcca
      541 gcaacgattc ggtggaacgc ggcaggcgca gcacgatctc gcgcagcgac acgtcgtcga
      601 gaatccagcc gcgcggccgg ttgcgttcgg cggcgcggcg ctcacgccag gcaccgagcg
      661 cctgggcgag ttcgtgccgc tgcggatcga gtgcgggcaa tcccttgatc ttcttccacg
      721 cctcttcggg cgcaacgcgc agtgcatcgg gattggcgag gcccgcgagt tcttcgtcga
      781 gccaggccac gcggcccttg gccgccagtg tttctaacaa gctcgcgcgc aatgcagcga
      841 gatggcgcac gtcgtcgagc gcgtattcct gctccgcggc cgacaacggc cggcgcgacc
      901 agtcggcgcg cgtgtgcgcc ttggcgagtt ccacgcccag caggcggcgc accagttcgc
      961 cgtaacccac ctgcgaagga tggcccgcga gcgccgcggc gatctgcgtg tcgaacaccg
     1021 gctggaccat gccgaccgcg ggatagagca cctcgaggtc ctgccgggca gcgtgcatga
     1081 tcttcacgcc gccggtagcg ccgagcagtg gcgtgagcac cgacaggtct ggactggcca
     1141 ggggatcgat gcatgcagcg ccgtcgccgg ttgcgatctg cacgaggcag agttccgcgc
     1201 gataggtttt ctcgcgcatg aattcggtat cgagcgccag cacgggcgcg ctcgccgcct
     1261 ggcgcgcgaa cgctgtcagc gcatcggaag tgtcgatcat caagcgggcc gttctccggg
     1321 tgacgtgtac aatcggcccg gcattatgct tgtgaatctc cgcgcattgt tcggccttct
     1381 ggtcgacatc gtcctcttgc gacgcgggcc ggagagtctc ccggcgtcgc cgagcgtgct
     1441 tgcgatcttc gtggtgctca acggcgccct gtcggcgctc atcgcctcgc tcatcccctc
     1501 gcatccggac atctcgatcg tggagctcat cgtgagcccg ttcgtcccca tgctgtggta
     1561 cctgatcgca ttctcgctcg cgaagaagcc cgagcggttc gtgcagacga tggtcgcgtt
     1621 cttcggcgtg aacctgctgt tccagcccgt ggtcgcgccg atgctcgccg cgctcacccc
     1681 gtacatggag aagcaggatc cggccatgcc gccgcccgcg gccctgagcc tgctgttcat
     1741 cgtgctgtcg gtatggctgc tgatcgtctg ggtgcgcatc gtccgcattg cgttcgagtg
     1801 gccgaccatc gtcgccttcg tcttcgtgtt ggcacaaaac ctcgtcgcga tcttcatcta
     1861 ttcggcgatg ttcggagttt cgtcaggaaa ggtttagggg ctacagttcg caccatgcac
     1921 gttcacattc tcggcatctg cggcaccttc atgggcggca tcgccgccat cgccaaggcc
     1981 gcgggccatc gcgtcaccgg ttccgacaag aacgtctatc cacccatgag cacacagctc
     2041 gaggcgctcg gcatcgagct gatttcggga ttcgacgcga accagctccg gcctgcgccg
     2101 gacgtggtgg tcgtcggcaa cgtcatgacg cgtggcgcgc cggtcatcga agcgctcctc
     2161 gagagcaata ttccgtacac ctccggtccc gagtggctgg cgcgcgaagt gttgcgcgac
     2221 gtccatgtgc tcggcgtatc gggaacccat ggcaagacca cgacctcgag catgctggcc
     2281 tggatcctcg agcacgccgg actcaatccc ggctttctca ttggtggtgt gcccggcaat
     2341 ttctcggtca gcgcgcggct cggtgggcga aagttcttcg tcatcgaagc cgatgaatac
     2401 gacacggcgt tcttcgacaa gcgtgcgaag ttcgttcact accgctcgcg cacgctgatc
     2461 ctcaacaatc tcgaattcga tcacgccgac atctacgaaa acctggcggc catccagaaa
     2521 cagttccacc acctggtgcg gaccattccc ggctccggcc gcgtcctgtg gaatgccggc
     2581 gatgaaaacc tgaaggccac gctcgcgatg ggctcctgga cgccgctcga aggcttcgcg
     2641 cgtacaccgg gcgccgacgt ggcctggtcg ctcgacgtgg aagagggcgg cgactattcc
     2701 aagttcgtgg tgctcgaagg cggcaaaccc gcgggcacgg tccgatggtc cttgttaggc
     2761 gcgcacaacg ccgagaacgc gctggccgcc atcggcgccg cgcgtcatgc gggcgtgtcg
     2821 gtggcgcagt ccatcgcggc gctggcggaa ttcaagggcg tgaaacgccg catggagttg
     2881 cgcggcgaag tgcgcggcat ttcggtctac gacgatttcg cgcatcaccc caccgccatc
     2941 gcctccacca tcgacggcct gcgccgcaag gtcgggggca agcgcatcat cgcggtgctc
     3001 gagccgcgct cggcgaccat gcgcatgggc gtgcatcgcg acacgctggc cccttcgctg
     3061 tccgcggcgg attccgtctg gatgttcgcg ccacccgatc tcggctggga tgccgatgcg
     3121 gtcgtcgcca ggctcggacc caagggcaag atcgcgcgtt cgctcgatga actggtcagc
     3181 gggctcgcgg gcaattccgc gagtggcgac caggtgctca tcatgagcaa cggcggtttc
     3241 ggcgggctgc acggcaaact actcgaggcg ctggccagaa tgtgagccga aagtgacaga
     3301 aggaagcgtc attgcgttgt ttccactgca caccgtgctc tttcccggcg gcccgctgcc
     3361 gctgcgtatc ttcgaaaccc gctacaccga catggtgagg cgctgcatgc gcgagcaatc
     3421 gccgttcggc gtcgtgctca tccaggacgg cgaagaagcc ggtgaagtgg ccaccaccgc
     3481 caccgtgggt tgcaccgcgc gcatcgccga tttccacacg ctgcacgacg gcctgctggg
     3541 catcagctgc gtgggcgacc gcaagttcct ggtcgagcgc gtgtggcgcg ccgccgatgg
     3601 tctcaacatg ggtgaggttt cgtggttgcc ggccgagcgc gtggtcgcgc tgcccgagcg
     3661 ttacggccac ctggcgacga ccgtgcggcg cgcgctcgaa gatctttccg agcactacga
     3721 atacatcgag aagaaattcg acgacgccgc gtggatcggc gcgcggctca cggagctcct
     3781 gcccatcgaa ctcgccgaca agcaggcgtt gctcgagctg gatgatccga tcgagcgcct
     3841 ggacaccctg ttgtccgtgg tgccctcgcc cgagtagcgt cagttctgct ttttcgcggc
     3901 aggtaccgcg atggccttgt ggccatgatt ccggagcagt tcgagcaggc cgtcacggcc
     3961 cacgaaatgc agcgcgccga ccaccacgag gtagtcgcga tcctgctcga gcagcgcctc
     4021 gatcttcggg agccactgac gattgcgcgt gcccagcaat gcgtcgtaga gctccggcga
     4081 tttcgcgcgc tccttgacga actccttctc gagcgcgcgc aggtctccgc cgcgccaggc
     4141 ggtgatgagc ttgtgcaggt cgtcattgag ccgcggcgca tcttcggtgg cgtcgagcag
     4201 gaaacgggtt tgctcttcga agctgcgatc gtcgaagacg ctcagctggt cgatgacggt
     4261 ttccagtccc tcgatgggct tgccgtcggc gcgtgcgcgt tcggtgatct gcatgtcgat
     4321 gccgagattc gcgtcgaagc cggtcttggt cagcgcgaac tgcgtgagca ccagggccgc
     4381 ggcccacggt tcgagtttcg caatgacgat ttccggaacc tcgagcgacg cggcgagttc
     4441 gctgattcgc tggtaggact tctcacccac cacctctttc agtgattggt cggcgggcaa
     4501 cgtgccgttc gtggtgatga acttcaccgc gtcgagcggg ttcatgtcgt cgagatcgac
     4561 ttccagcacg atgacctgcg cggccgcgta ggcgcggtcg agctgctcgg ggaaatccga
     4621 atgacccttg ggcagcgcgt gtaccgagcc cgcgaggtag acggtgttgc gtgtgccttt
     4681 catcgaccaa acggaagttt cgccgcgggc atgcgccgtt tgcacgccaa tcgcgagcaa
     4741 cagcacaccc gcgaggccgc gggcgaggcc tcgggacatc gctttgggga aaatcatgcc
     4801 gcggattctg cggcagtcag tgagtgcacg ccagtgtcga tggcgccgtt ggagtggagc
     4861 gtcagcagcc ggtaggccgg cggctggttg tcgatgacga actgatcgga gcgcggctgg
     4921 aactgcgcgc aggtggaagg cgtggcgaac aggcgcacgc cacgacgggc gccttcgaag
     4981 ttctgatgca cgtgacccca caccaccgcc ttcacctgtg gatggctgtc gatgaccgac
     5041 cagaattcgt cggcattgga caagcccacg ctgtcgagcc agcggctgtt catcgcgatc
     5101 gggtgatgat ggaggcagac catcgcgtgg cgctgacgcg cgcggcccag gtcttcgtcg
     5161 agcttggtga gcatctcgac ggagatgcgg ccgcccgcct gatcgggcac cacgctgtcg
     5221 agaagcacga tgcgccaggc gccgatgtcg gccggaccgt ccatgtcgaa aggacgcccc
     5281 gcgagcgccg cgcgcatctg cggaacgaaa tcgtgattgc cgggaataca cagcaccggt
     5341 ttgccgaagc gcgcgaacag gcgtcggaag tgttcgtagc cgccggcgtc gtcctgcacg
     5401 agatcgccag tgacgaggat ggcgtcgctc atggcgatat ggcccgacgc gtcagcgagc
     5461 gtgcgttcca gtgtttcgaa ggtgttcacg ccacgcagcg ttccggcgat gtcgccgaac
     5521 aagtgagggt cggtgaactg gatgactctg atggcactca ttcattcgaa ctctacaaac
     5581 atgccgggga ggggccggtg atgcacgtca cgtcacgctt catggcactg taacgtggtg
     5641 aaccaggtcg caaaccaccc ccgggataat gtgaagcagc gactgcaaat agcgactttc
     5701 cgaacatgta accgctatca gaacaatggt aaatcggact acggatattc cacggctgat
     5761 tggactgcgg aatcgtgctt gcgtgcccga cgcgagcgtt taaagtgtcg gctcacatga
     5821 agagacccgg caagagtgat ccgagtcggc cgcgggaaga cggctacgac tacaagcgcg
     5881 agttctatcg gagctcgtcg gttgccgagg actatgactt ccaccgcttc agcacgcccg
     5941 agcgtcagaa gcgtaacgcc cgaaagtggg ccgcgatccg cgcggcgctg cgcgaaacca
     6001 ccggtgttag aaccatcctc gacctgcctt gcggcaccgg ccgcttcacc ggcgcgctcg
     6061 cgcgcgaagg cttcgagatc gtcggtagcg acatttcgca cgaaatgctc cacaaggccg
     6121 cgtcgctcga cgggctgccg caggaaatcc gcggtttcgt tcaagccaat gccgagcacc
     6181 tgccgctgcg caacgattcg ctggattgcg tcgtgagcat ccgcttcatg atgcacgtcg
     6241 accctgtctc gcgcgtgcgc atgctgcgtg aattccatcg tgtctcgcgg cgctgggtca
     6301 tcgtcgatta ccgccactgc tactcgttcc gttacctgct cacgcacacc ttcggaagaa
     6361 tcggcatcgg ccgcccgccg ttgtcgcgcg tgtcacgcaa ggaactcgag aaagagtttt
     6421 ccgacgccgg gtttgcgatc cgcaaggtca tccgcgtgtc ggcgccgctg ctgtcggaca
     6481 agtggatagt gctcgccgag cgggcctgac tgttcctggc gccgggcggg acccgggcgt
     6541 gaaaaacgct caattcgacc ttcgtcggtg cacgtcgccg tacacccggg ccgtgaacag
     6601 caaatgtcca ccgagacaat ggctcttccg ggtgagtttc cccgcattca ggttgccccc
     6661 gtgagcctgc ct
//
LOCUS       DATDEU010000552         7589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_690932, whole genome shotgun sequence.
ACCESSION   DATDEU010000552 DATDEU010000000
VERSION     DATDEU010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690932"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..642)
                     /locus_tag="VIV63_13165"
     CDS             complement(193..642)
                     /locus_tag="VIV63_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY6125596.1"
                     /translation="MKTEPSTFGIDDLAGKPKKTAMWDGVRNYQARNMLRDDFRKGDQ
                     AFLYHSSCEVPGVAGIVEVVRTAYPDPTAFDKKHDHYDEGSDPKNPRWVVVDVQLKRR
                     LKRVITLDELRKHETRELDGMLLLKRGNRLSITPLSAAHWKFILSLE"
     gene            complement(608..1222)
                     /locus_tag="VIV63_13170"
     CDS             complement(608..1222)
                     /locus_tag="VIV63_13170"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY6125597.1"
                     /translation="MQTKSTLRIEILSRRRAVSRAARKVAARTVADIVARTFWLSPGK
                     RIGIYTSMPNELGTAPLIALALARGCEVYVPRITSLRARTMRFVKFSDRGTRHALGMH
                     EPDNDQSLHARFLDTLFVPAVALDRRGARLGHGLGFYDRTLAFRRLRRHWRGPRLVAL
                     AYSFQVVPQVPVTATDVFMDLIVTERGIDELLAHEDRTIDLRDR"
     gene            complement(1238..1424)
                     /gene="ssrS"
                     /locus_tag="VIV63_13175"
     ncRNA           complement(1238..1424)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VIV63_13175"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1533..1850)
                     /locus_tag="VIV63_13180"
     CDS             complement(1533..1850)
                     /locus_tag="VIV63_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY6125598.1"
                     /translation="MSEKESATVTVRILEKEYFVSCPQDERADLLDSAEYLNKKMREI
                     RDTGKVVGADRIAVMAALNMANELLRLRKQDTDLQGSVTGRVKNMRERVEGALQRTRQ
                     LDL"
     gene            complement(1847..2095)
                     /locus_tag="VIV63_13185"
     CDS             complement(1847..2095)
                     /locus_tag="VIV63_13185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HEY6125599.1"
                     /translation="MNDTSDNNKTGKSGFSAEISLLERRIEELLLTVDTLKEENRALR
                     QRQESLAAERSAFAQRNDQVRARVEAMIGRLKTMEHTA"
     gene            2208..2774
                     /locus_tag="VIV63_13190"
     CDS             2208..2774
                     /locus_tag="VIV63_13190"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEY6125600.1"
                     /translation="MLPATYDEFEQVLREARALPEPSEAHGTLAGALCSSRDYGLIEW
                     VREILPDDSPEGDALQSSVLQNVYNAMVRTLVGSDADFEPLLPDDDSPLTMRADALSQ
                     WCQGFLYGLGSGTTGDPGKVSVEAGEIIRDLTEITHVGVDADEETEENEVAFAEVVEF
                     VRVGVQLLFAELAPARGEEPEPGAASIH"
     gene            2815..4134
                     /gene="pepP"
                     /locus_tag="VIV63_13195"
     CDS             2815..4134
                     /gene="pepP"
                     /locus_tag="VIV63_13195"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF008131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEY6125601.1"
                     /translation="MPSIGREEFKRRRKALLKQMGRDSIAIVPTAPVRLRNNDVEYAY
                     RPDSDFFYLTGFVEPESVAVLVPGRPQGEYILFVRDRDPARETWDGRRAGPDGATRDF
                     GADDAFPISDIDEILPGLLENRGKVFYAMGTHPEFDQRVVGWVNGLRTQARNGRHAPQ
                     EISALDHVLHDMRLFKSRAEVDTMREAARIAADAHIRAMKACAPGKREYEIAAEIVHE
                     FRMHNADLSYLPIVGGGANGCILHYRENDAALRDGDLLLIDAGCELDCYASDITRTFP
                     INGRFSPEQRVLYDIVLEANYAAIERVKPGNHWNEPHEAAVRVITQGLVKAGLLKGKP
                     AKLEESGAYRKYFMHRTGHWLGMDVHDVGDYKIGNEWRVFEPGMALTIEPGIYVAPGT
                     PGAPKKFAGIGIRIEDDVVVTREGCEVLTQGCPKEAVEIERLMASVA"
     gene            4159..5382
                     /gene="ubiH"
                     /locus_tag="VIV63_13200"
                     /gene_synonym="visB"
     CDS             4159..5382
                     /gene="ubiH"
                     /locus_tag="VIV63_13200"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEY6125602.1"
                     /translation="MKEWDVAIVGGGMVGASFALALRSTPLRVLLIESIPPDSAAQPS
                     FDERTTALGNGSRQIFETLGVWPAMSGEASPIRSIHVSDAGRFGVARLDAREQGVPAF
                     GYVVPNRVIGRVLWQALREAPNVTVAMPAQLKSATLRDDGVLLDVVIEGVTERVRAAV
                     AVAADGAGSVLRASAGIDAAVEDYEQIALVVNAATERPHTGEAFERFTSSGPLAVLPV
                     TGGGYAVVWAVTPARAAELISLDEAGFAAELLSAFGWRAGRWTRIGRRNSYPLALTRA
                     EETVAGRVVLIGNAAQALHPVAGQGFNLGLRDAATLAEMLASAGTGPDFSATAAELLE
                     KFAGWRAEDRKGVTRFTDGLVKLFGSDMPGIGLVRNFGLLLFDLSPAAKRALSRVSWG
                     FAGRMPRLARGLPLE"
     gene            5379..6605
                     /locus_tag="VIV63_13205"
     CDS             5379..6605
                     /locus_tag="VIV63_13205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6125603.1"
                     /translation="MSIDYDVAVVGAGPIGLATAILIARKAGIPPARVAVFDRRIPDS
                     LDGAKELPVDLRVFALSRASEKILRSADAWSDIEATRAEAFERMHVWHADVPPHGGDA
                     LVFDAAEMGERNLGVIAENAVLQAALAGAARRAGVALITGDISELVQERDAVVLVAAE
                     RQVRARLVIGADGAQSRVRELTGLGAARTDYGQTAIVAMVSTARSHEHTAWQRFLGDG
                     TLAFLPLKDAHSSIVWSVPTARAERLLAATAEVFERELESDFDGALGKVRLVSERVKF
                     PLWRLSANAYVTARVALVGDAAHVVHPLAGQGANLGLLDAAALADVLAEGVRSREDPG
                     AERILRRYERWRRSENDLMSGAIDAFDRLLARGTGRIAELAQRGMPWVGRSALAKRMF
                     IERAMGLAGELPTAAH"
     gene            6615..7487
                     /locus_tag="VIV63_13210"
     CDS             6615..7487
                     /locus_tag="VIV63_13210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6125604.1"
                     /translation="MDEQKQHRLAGMAAMLAGVAAFSFMDAGLKLLTAHYPAAQVAAL
                     RGLSALPLVFIWALYAGGATQLVRVRWPLHLIRGVLSVLMMISFTFALKELSLAKTYA
                     LFFIAPLLIAILSTFMLGERVLRTQWVAIVVGFAGVLIVLKPGAVGFAWAGTLAVLGT
                     AFCYALSSVLVKIIGRTDTTQAMVFWMTCMLAIGATAIALPGWQPILEEHYLIIGGVA
                     ITGAVGQWGITEAFKHAPAASVAPLEYSGLAWVIMIDFFVWSVTPEIRTLAGAAVIIA
                     SGLYLLRFEARRSS"
BASE COUNT         1300 a         2362 c         2518 g         1409 t
ORIGIN      
        1 cgccttttgg cacaaggcca gaagaattac ttcttcttgg ctttcttctt ggcctttttc
       61 ttggctttcg ccatggcgca tctccagtag ttacgggtta gtccgagggc gagtatacgc
      121 aggcgttgac aaatttcaag caagtgtcat cgaacggaca acgcgtgaat cacatcgacg
      181 tgttcatcaa catcactcga gcgagaggat gaacttccaa tgagcggcac tcagaggtgt
      241 gatcgagagt ctgttgccgc gtttcaacag cagcatccca tctagttcgc gcgtctcgtg
      301 cttgcgcagt tcatcgagcg tgatcacgcg cttcaggcgc cgtttgagtt gcacgtcgac
      361 cacaacccag cgcggattct tcggatcact gccttcatcg tagtggtcgt gcttcttgtc
      421 gaaggccgtg ggatcgggat acgcggtgcg taccacctcg acgatgcccg ctacacctgg
      481 aacctcgcag ctcgagtgat agaggaacgc ctggtccccc ttcctgaagt catcgcgcag
      541 catgtttcgt gcctggtagt tgcgcacgcc gtcccacatg gcggtcttct tcggcttccc
      601 ggcgaggtca tcgatcccga aggtcgatgg ttcggtcttc atgagccaat agttcatcga
      661 tacctctttc cgtgacgatg agatccatga acacgtcggt cgcagtcacg ggtacctgcg
      721 gcacgacctg gaaagaatag gcgagggcca cgagacgcgg gccgcgccag tggcggcgca
      781 ggcgacggaa cgccagcgtg cgatcgtaga accccaatcc gtgaccgagc cgcgcgccgc
      841 gacggtcgag cgcgacggcc gggacgaaca gagtgtcgag gaatcgcgcg tgcagggatt
      901 ggtcgttgtc gggctcgtgc attccaagcg cgtggcgcgt accgcggtca ctgaacttca
      961 cgaatcgcat ggtgcgggcg cgtaacgagg tgatgcgcgg cacgtagacc tcgcatccgc
     1021 gcgccagggc gagcgcgatc agaggcgcgg tgccgagctc attcggcatc gaggtataga
     1081 tgccgatgcg tttgccgggt gagagccaga aggtgcgcgc gacgatgtcg gcgactgtgc
     1141 gtgcggccac tttccgcgca gcacgcgaga ccgcgcggcg gcggctcagg atttcgatgc
     1201 ggagagtgga cttcgtttgc acgaggtgga aggaaaagcg ctacccgaaa gtgccggtgc
     1261 aagccattgc tctcgaacca gagcaacaag gtgggaccgg ccctgacatt taaggctttc
     1321 cgccaagggc ggacctgcac aatgctgtca gaaagcgttg gtcccgggcg tttacattac
     1381 ggtcgagagg taacccgact tactgtcgaa caccacaggt agcgcaggag tgactctaca
     1441 ccaaccgcgc cgtaggaatg cgacgcaagt attactcagg gttaccgccc acgtcggaaa
     1501 acgccgtcga tgcgacggcg tccgcaaata ttctagagat ccaactggcg tgtgcgttgc
     1561 agagcgcctt cgacacgttc acgcatgttc ttcacgcgac ccgtgacgct gccttgcaaa
     1621 tcggtgtcct gcttgcgaag acgcagcagt tcattggcca tgttgagcgc ggccatcacg
     1681 gcgatgcgat ctgcgcccac cacttttccg gtgtcgcgga tctcgcgcat cttcttgttg
     1741 aggtattcgg cggagtcgag aaggtcggcg cgttcgtcct gcgggcagga aacgaaatat
     1801 tccttttcca ggatccgtac ggtgacggtg gccgattcct tttcgctcat gcagtgtgct
     1861 ccatcgtttt caggcggccg atcatggcct ccacacgggc gcgcacctgg tcatttcgct
     1921 gtgcgaacgc ggagcgttcg gcggcgagtg attcctggcg ctggcgcagg gcgcggttct
     1981 cttccttgag agtgtcgaca gtgagcagaa gttcttcgat gcgtcgttcg agcagcgaga
     2041 tctctgccga gaaaccgctt ttgccggtct tgttgttatc agatgtgtcg ttcatgggcg
     2101 gggaatatat ggcacccagc gccggcccgg caaaaaatca tggacgccgc cccttgaggg
     2161 tggcgttttc cgttgtggcg tttacggctg gcaatcataa gtcccccatg ttgccggcca
     2221 cttacgacga gttcgagcag gtgctcaggg aagcccgtgc gctgccggag ccgtccgaag
     2281 cacatgggac gctggcgggc gcgctctgca gctcgcgcga ttacggtctc atcgaatggg
     2341 tgcgcgagat acttccggac gactcacccg agggtgatgc cctgcaaagt tcggtgctgc
     2401 agaacgttta caacgccatg gtccgcacgc tggtaggcag cgacgcggat ttcgaaccgc
     2461 tgctccccga tgacgattca ccgcttacca tgcgcgccga cgcgctgagc cagtggtgcc
     2521 agggctttct ctatggcctc ggatccggca ccaccggcga ccccggaaag gtctccgtcg
     2581 aagccggcga aatcatccgc gatctcaccg agatcactca tgtcggtgtc gatgcggacg
     2641 aagagaccga ggagaacgaa gtcgccttcg ccgaagtggt ggagttcgtg cgcgtcggcg
     2701 tacaactcct gttcgcggag ctggcgcccg cccgtggcga ggagccggag cccggtgccg
     2761 cgtctatcca ttagcaagct cagccctcgc agatcagcgc cgagcagggc gcgcgtgccc
     2821 tcgatcgggc gcgaagaatt caaacgccgc cgcaaggcgc tgctcaagca gatgggtcgc
     2881 gattccatcg cgatcgttcc cacggcgccc gtgcgcctgc gcaacaacga cgtcgaatac
     2941 gcgtaccggc ccgacagcga cttcttctat ctcaccggat tcgtggaacc cgagtccgtg
     3001 gctgtgctcg tgccgggccg tcctcagggt gaatacatcc tgttcgttcg cgatcgcgat
     3061 ccggcgcgcg agacctggga tggccggcgc gccggccccg atggcgccac gcgcgacttc
     3121 ggcgcggacg acgcattccc tatctccgac atcgacgaga tcctgccggg gctgctcgag
     3181 aatcgaggca aagtgtttta cgcgatgggc acgcatccgg aattcgacca gcgcgtcgtc
     3241 ggctgggtga atggcctgcg tacccaggca cgcaatggcc ggcatgcccc gcaggaaata
     3301 tcggcgctcg atcacgtgct gcacgacatg cgcctgttca agagccgcgc cgaggtcgac
     3361 accatgcgcg aggctgcgcg tatcgccgcg gacgcgcata tccgcgccat gaaggcgtgc
     3421 gcgccgggca aacgcgagta cgagatcgcc gccgagatcg tccacgaatt ccgcatgcac
     3481 aacgccgacc tgtcctatct tcccatcgtc ggcggcggcg ccaacggctg catcctgcat
     3541 taccgcgaaa acgatgcggc actgcgcgat ggcgatctgc tgctcatcga cgcgggctgc
     3601 gaactcgatt gttatgcctc cgacatcacg cgcacgttcc cgatcaacgg ccggttctcg
     3661 cccgagcagc gcgtgctcta cgacatcgtg ctcgaggcca actacgccgc catcgagcgc
     3721 gtgaagcccg gcaatcactg gaacgagccg catgaggcgg cggtgcgcgt catcacgcag
     3781 ggcctggtca aggccgggct cctcaagggc aagcccgcga aactcgagga gagcggcgcc
     3841 tatcgcaaat acttcatgca tcgcaccggc cattggctcg gcatggacgt gcacgacgtg
     3901 ggcgactaca agatcggcaa cgagtggcgc gtattcgaac cgggcatggc gctcaccatc
     3961 gagcccggta tatacgtggc gccgggtacg ccgggcgcgc cgaagaaatt cgccgggata
     4021 ggtatccgca tcgaggacga cgtggtggtc acgcgcgaag gttgtgaggt cctgacccag
     4081 ggctgcccca aggaagccgt cgagatcgag cggctcatgg cgtccgtggc atgaaaaccc
     4141 ttccatgaaa gtccttccat gaaagagtgg gacgtcgcga tcgtcggcgg cggcatggtc
     4201 ggtgcgagtt tcgcgctggc gctgcggtcg acccccttgc gcgtcctgct catcgagagc
     4261 atcccgccgg attcggcagc gcaaccgagc ttcgacgaac gcaccacggc gctcggcaac
     4321 ggatcgcgac agatattcga gacgctcggc gtatggccgg cgatgtcggg cgaggcatcg
     4381 cccatccgct ccatccacgt ctccgatgcg ggacgcttcg gggtcgcacg gctcgatgcg
     4441 cgcgagcagg gtgtccctgc attcggctat gtggttccaa accgcgtcat aggccgcgtg
     4501 ttgtggcagg cgctgcgtga agcgccgaac gtcacggttg ccatgccggc gcaattgaag
     4561 agcgcgacgc tgcgcgacga cggcgttttg ctcgatgtag tcattgaggg tgtgaccgag
     4621 cgggttcgcg ccgcggtcgc cgtggcggcg gacggtgcgg ggtcggtctt gcgcgccagt
     4681 gccggcatcg atgcggccgt cgaagactac gagcagatcg cgctcgtggt gaatgccgcc
     4741 accgaaaggc cccatacggg cgaggccttc gaacgattca cttcttcggg tccgctcgcg
     4801 gtgttgccgg tgacgggcgg tggttacgcc gtcgtatggg ccgtgactcc tgcgcgcgcc
     4861 gccgaactca tctcgctcga cgaagccggt ttcgccgcgg aactgctcag cgcgttcggc
     4921 tggcgtgcag gacgctggac gcgcatcgga cgccgcaact cctatccact cgcgctgacg
     4981 cgcgccgagg aaaccgtcgc gggacgtgtc gtgctcatcg gcaacgccgc gcaggcgctg
     5041 catccggtgg ccggccaggg tttcaatctg ggcctgcgcg atgcggccac gctggcggag
     5101 atgcttgcca gtgcgggcac gggtcctgat ttctcggcga ccgcggccga gctgctcgaa
     5161 aagtttgccg ggtggcgcgc ggaagaccgc aaaggtgtca cgcgcttcac cgacgggctc
     5221 gtgaaactct tcggcagcga catgccggga atcggactgg tgcgcaattt cggcctgttg
     5281 ctgttcgacc tgagtcccgc ggccaaacgc gcgctgtcgc gcgtgagctg gggcttcgcc
     5341 gggcgcatgc cgaggctggc tcgcggcctg ccgctcgaat gagcatcgac tacgacgtgg
     5401 ccgtggtggg tgcgggcccc atcggtctgg ccaccgccat cctcattgct cgcaaggccg
     5461 gcattccgcc agcgcgcgtc gcggtgttcg accggcgtat tccggattca ctcgacggcg
     5521 cgaaagaact gccggtcgac ctgcgcgtgt tcgcgctgtc gcgcgcgagt gaaaaaatcc
     5581 tgcgcagcgc ggatgcctgg agcgacatcg aggccacgcg cgccgaagcc ttcgaacgca
     5641 tgcacgtgtg gcacgccgac gttccgccgc atggcggcga cgcgctggtg ttcgacgcgg
     5701 cggagatggg cgaacgcaat ctcggtgtca tcgccgagaa cgcggtgctg caggccgcgt
     5761 tggcgggcgc cgcgcgtcgc gcgggtgtcg cgctcatcac cggcgacatc agcgagctcg
     5821 tacaggagcg cgacgccgtg gtgctcgtgg ccgccgaacg ccaggtgcgt gccaggctcg
     5881 tgatcggcgc cgatggcgcg caatcgcgcg tgcgcgaact caccggcctg ggcgcggcac
     5941 gcaccgacta tggccagacc gccatcgtcg ccatggtgag cacggcgcga tcacacgagc
     6001 acaccgcgtg gcagcgattt ctcggtgacg ggacgctggc tttcctgccg ctgaaggacg
     6061 cgcacagttc catcgtctgg tcggtgccca cggcgcgcgc cgagaggctg ctcgcagcca
     6121 cggcagaagt cttcgaacgc gaactcgaat ccgatttcga tggcgcgctc ggcaaggtgc
     6181 ggctcgtgag tgagcgcgtg aaatttccgt tgtggcggtt atcggccaat gcctatgtga
     6241 ccgcgcgcgt ggcgctggtc ggagatgccg cgcacgtcgt gcatccgctc gccgggcagg
     6301 gtgccaacct cggattgctg gatgcggcgg cgctggccga cgtgctggcc gagggcgtga
     6361 ggagccggga agatccggga gccgaacgaa ttctcaggcg ttatgaacgg tggcggcgca
     6421 gcgaaaacga tttgatgagc ggcgccatcg atgcgttcga ccgcctgctg gcgcgcggca
     6481 ccggccgcat cgccgagctt gcgcaacgcg gcatgccctg ggtcggccgt tccgccctgg
     6541 caaaacgaat gttcatcgag cgcgccatgg ggctcgccgg agaactgccg accgcggcgc
     6601 actgacccgt tcccatggac gaacagaaac agcaccgcct ggcgggcatg gcggcgatgc
     6661 tcgcgggtgt cgccgcgttc tcgttcatgg acgcggggct caaactgctg acggcccact
     6721 atcctgcggc gcaggtggcg gcgttgcgcg ggctatcggc gttgccgctg gtgttcatct
     6781 gggcgctgta cgcgggcggt gcaacgcagc tcgtgcgcgt gcgctggccg ctgcatctca
     6841 ttcgcggcgt gttgtcggtg ctcatgatga tcagcttcac gttcgcgctg aaagagctgt
     6901 cgctcgccaa gacctacgcg ctcttcttca tcgcgccgtt gctcatcgcc atcctgtcca
     6961 cgttcatgct gggcgaacgc gtgctccgca cgcagtgggt ggcgattgtc gtgggtttcg
     7021 ccggagtact catcgtcctc aagccaggcg ccgtgggttt tgcctgggca ggaacgctgg
     7081 cggtactggg cacggccttc tgctacgcgt tgtcgtcggt gctggtgaag atcatcggcc
     7141 gcacggacac cacgcaggcc atggtgttct ggatgacctg catgctggcg atcggcgcga
     7201 cggccattgc actgccgggc tggcagccca tcctcgagga gcactacctc atcatcggcg
     7261 gagtcgcgat taccggcgcc gtcgggcaat ggggtatcac cgaagcgttc aagcacgcac
     7321 cggcggccag cgtggcgcca ctcgaatatt cgggcctcgc ctgggtgatc atgatcgact
     7381 ttttcgtctg gtcggtgacg ccggaaatac gcacgctggc gggcgccgcc gtgatcatcg
     7441 ccagcggtct gtacctgctt cgtttcgagg cgcggcgttc aagttaggcg cgggtggaaa
     7501 tggggacatt cctcatttgc tagcaaatag ggacgcgcct cagagaggca cgtccctttt
     7561 tgccaaatcg cgccacgaat cgtgacgct
//
LOCUS       DATDEU010000553         5942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1130697, whole genome shotgun sequence.
ACCESSION   DATDEU010000553 DATDEU010000000
VERSION     DATDEU010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130697"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /gene="htpX"
                     /locus_tag="VIV63_13215"
     CDS             <1..727
                     /gene="htpX"
                     /locus_tag="VIV63_13215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY6125605.1"
                     /translation="GAFISLLISKWMAKRAMGVQVIKEPANETERWLVNVVTQQAQTA
                     GIGMPEVGIFDSPEPNAFATGANKNAALVAVSTGLLRNMRREEVEAVLGHEVSHVANG
                     DMVTLTLIQGVMNTFVFFLARVIGGIVDKVILKNERGNGIGYFVTVMAAQIVLGFLAG
                     MVVAWFSRQREFRADAGGAHLAGTNSMIGALEALKRVHTPTAMPQQMKAFGIREGEVS
                     GFRRLLMSHPPLDERIAALNSAR"
     gene            850..1833
                     /locus_tag="VIV63_13220"
     CDS             850..1833
                     /locus_tag="VIV63_13220"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY6125606.1"
                     /translation="MNADTQTSNNPLTRMTRAVLHWFDTARASSFDADHAGADRIDWI
                     RTLPFILMHAACLFAFVVGVSPVAILVCVALYFVRMFAITGFYHRYFSHRSFKTSRVA
                     QFLFGVLGSSAVQRGPIWWAAHHRDHHQYSDKKEDAHSPIQHGFLRAHMSWFLSKKGF
                     APDLKRVRDLMNFPELRWLDRFDIIVPVLLAVAVFFLGVALEKWAPGLGTNGWQMLVW
                     GFFVSTVLTSHGTYTINSLSHVFGKQRYKTGDSSRNNWFLALITLGEGWHNNHHHYPS
                     STRQGFYWWEVDITYYLLKVMSWFGLIWDLKPVPVSVRDHHPRRIDRQGVQ"
     gene            1909..3207
                     /locus_tag="VIV63_13225"
     CDS             1909..3207
                     /locus_tag="VIV63_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6125607.1"
                     /translation="MRVAIIGSGIAGMVAAARLHPRHDVTVFEAGAYVGGHTNTIDVD
                     CQGRKFAVDTGFIVFNDWTYPRFIALLNELGVAYQSSNMSFSLRDERNGLEYNGTSIN
                     TLFAQRLNALRPSFLRMIADILRFNKQSRDLLAGSDDTLTLGEYLEANRYSRAFREQF
                     IVPMGMSIWSSTERAMLSFPARFFVEFFDKHGFLNVDDRPVWQAVKGGSREYMRKLSA
                     PLAHRIRLETAVTAVRRDAAGVTVRTAMGSVERFDQVVIACHSDQALKLLQDASPAEQ
                     EILSAFPYQENEAVLHTDESLLPRAPLARAAWNYHLLAVRDNAMRDRVALTYDMNVLQ
                     SLDAPVRFLVTLNRTADIDPAKVLKTITYHHPVYLPAGVAAQKRRAEISGVNRTFYCG
                     AYWRYGFHEDGVVSAEWVLEEFARLAQSAPFESARLARAV"
     gene            3214..3993
                     /locus_tag="VIV63_13230"
     CDS             3214..3993
                     /locus_tag="VIV63_13230"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEY6125608.1"
                     /translation="MKSALYSGWIQHRRFGPARNSFRYRIFMSYIDLAELPGLFKGRW
                     FWSARRPSLAWFRRRDFLGPSHLPLDCAVRDLVERHTGARPTGPIRLLTHLRHFGYSF
                     NPVSFYYLFDANDTRVETIVAEITNTPWKQRHAYVLPVSTAAHGARTAAWRFSFEKQF
                     HVSPFMPMDVRYEWCFGAPAQGLHVHMENWRAGQADFDATLNLHREEMTAGSLARALI
                     QFPLITAKVSALIYGQALKLLWKRAPFHSHPDKVETGFTHP"
     gene            3990..5279
                     /locus_tag="VIV63_13235"
     CDS             3990..5279
                     /locus_tag="VIV63_13235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY6125609.1"
                     /translation="MNTRSPLLLESLVSTGPSNPTLLARLGRKLLLGQLRQIEHGELR
                     LVEPDGRQHVFGKRHAAFDLGCTVYFDNPQSFADAAFGGTVGAGEAYIRGLWRCDDLV
                     TLVRIFVANREQTNRIDSRWSLVSRPLLRLFHWANRNSRHGSARNIAAHYDLGNELYQ
                     LMLDDTMAYSCAIFPHAGATLAEGSEAKFEAVCRKLDLKPTDHVLEIGTGWGGFAIHA
                     VQRYGCRVTTTTISKSQAEFAVRKIAALGLSDRITVLLEDYRDLKGDLAGPYDKLVSI
                     EMVEAVGARFLDGYFRHCSRLLKPEGAMLLQSITLQDQFYDQALKSVDFIQRYVFPGS
                     FIPSVQALSKSLAKSTDLKIFHLEDIGPHYARTLRLWRERFFRNLNTVRELGYSESFI
                     RLWEYYLSYCEGGFEERQIGTVQMLLTKPRCRRQPIR"
     gene            5320..>5942
                     /locus_tag="VIV63_13240"
     CDS             5320..>5942
                     /locus_tag="VIV63_13240"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6125610.1"
                     /translation="MNDLAHSLHGLRALVDDATILTDPERIAPMLRDHRALYRGHALA
                     VVAPADTATVSRVLAFCNEHHIGVVPQGGNTSYCGGATPDASSSQVLLSLARMNRVRA
                     VDATNYAMIAEAGCVLAHLQAAAAEAGRYLPLSLGSEGSCMLGGNLSTNAGGLNVIHY
                     GMARELVLGLEVVLADGRVMETLTALRKDNTGYDIKSLFLGAEGTLGV"
BASE COUNT         1028 a         2042 c         1792 g         1080 t
ORIGIN      
        1 cggcgcgttc atctcgctgc tcatctccaa gtggatggcg aagcgcgcca tgggcgtgca
       61 ggtcatcaag gaaccggcga acgaaaccga gcgctggctg gtgaacgtgg tcacgcagca
      121 ggcccagacc gccggcatcg gcatgcctga agtcggcatc ttcgactccc cggagccgaa
      181 cgcgtttgcc acgggcgcca acaagaacgc cgcgctggtg gcggtgagca ccggcctcct
      241 gcgcaacatg cgccgtgaag aagtcgaggc ggtgctgggt cacgaggtga gccacgtcgc
      301 caacggcgac atggtcacgc tcacgctcat ccagggcgtc atgaacacct tcgtgttctt
      361 cctcgcgcgc gtcatcggcg gcatcgtcga caaagtcatc ctcaagaacg agcgcggcaa
      421 cggcatcggc tacttcgtca ccgtcatggc ggcgcagatc gtgctcggct tcctcgccgg
      481 catggtggtc gcctggttct cgcgccagcg tgaattccgc gcggacgcgg gcggcgcgca
      541 tctcgcgggt accaattcga tgatcggcgc actcgaagcg ttgaagcgcg tacatacccc
      601 tacggcgatg ccgcagcaga tgaaggcctt cggtatccgc gagggtgagg tgtcgggttt
      661 ccgtcgcctg ctcatgagcc acccgccgct cgatgagcga attgccgcac tgaacagcgc
      721 gcgctaactg ccaagcaatt caaagcttgc gccagccggt cgttgcagac ttccggctgg
      781 cgttgccttg acggcgcgca tccaggaccc cgcgcgacac cgtattccca gaaggattcc
      841 gcgagactca tgaacgcaga cacgcaaacc tcgaacaacc cgctgacccg catgacgcgt
      901 gcggtgctgc actggttcga taccgcccgg gcctcgagct tcgatgccga tcacgccggc
      961 gcggaccgca tcgactggat tcgcacgctg ccgttcatcc tcatgcacgc ggcttgtttg
     1021 tttgccttcg tggtgggcgt cagcccggtg gccatcctgg tgtgcgtggc gctgtacttc
     1081 gtgcgcatgt tcgccatcac cggcttctat catcgctact tctcgcacag gtcgttcaag
     1141 acctcgcgcg tggcgcagtt cctgttcggc gtgctcggct cgtcggccgt gcagcgcggg
     1201 ccgatctggt gggccgccca ccatcgcgac catcaccagt actccgacaa gaaagaagac
     1261 gcgcactcac ccatccagca cggcttcctg cgcgcgcaca tgagctggtt cctctcgaag
     1321 aaaggtttcg cgcccgacct caagcgcgtg cgcgatctca tgaacttccc cgagctgcgc
     1381 tggctcgacc gtttcgacat catcgtgccc gtgttgctgg ccgtcgccgt gttcttcctc
     1441 ggtgtcgcgc tcgaaaaatg ggcgccgggc ctcggcacca acggctggca gatgctcgtc
     1501 tggggcttct tcgtgtccac cgtgctcacc tcgcacggca cctacaccat caattcgctg
     1561 tcgcacgttt tcggcaagca gcgttacaag accggcgact cgagccgcaa caactggttc
     1621 ctggcgctca tcacgctcgg cgaagggtgg cacaacaatc accaccacta cccgagctcg
     1681 acgcggcagg gtttctactg gtgggaagtc gacattacct attacctgct caaggtcatg
     1741 tcgtggttcg ggctcatctg ggatctcaag ccggtgcccg tgtcggtgcg cgatcaccac
     1801 ccgcgccgga tcgaccggca aggcgtccaa tagcctccta ggccccgcgc atccgaaagc
     1861 cccgaactcc cgtacctacg ggggagttcg gccactttcg ggttattcat gcgcgtcgcg
     1921 atcatcggtt ctggcattgc gggcatggtc gccgccgcgc ggctgcaccc gcgccacgac
     1981 gtcacggtct tcgaagccgg cgcctacgtg ggcggacaca ccaacaccat cgacgtcgac
     2041 tgccagggcc gaaagttcgc ggtcgacacc ggcttcatcg tcttcaacga ctggacctat
     2101 ccgcgcttca tcgcgctgct gaacgagctc ggcgtcgcgt accagagctc caatatgagc
     2161 ttcagcctgc gcgacgaacg caacgggctc gaatacaacg gcacgagcat caacacgttg
     2221 tttgcccagc ggctgaacgc gctgcggccg tcgttcctgc gcatgatcgc cgacatcctg
     2281 cggttcaata agcaaagccg ggacctgctg gccggcagcg atgacacgct caccctcggt
     2341 gaatacctgg aggcgaaccg ctactcgcgc gctttccgcg aacagttcat cgtccccatg
     2401 ggcatgtcga tctggtcgtc gacggaacgc gcgatgttgt cgttcccggc gcgtttcttc
     2461 gtcgagttct tcgacaaaca cgggttcctc aacgtggacg accgcccggt gtggcaggcc
     2521 gtcaagggcg gctcgcgcga atacatgcgc aagctcagcg caccgctcgc ccatcgcatc
     2581 cgtctggaga ccgcggtcac ggccgttcga cgcgacgcgg ccggggtcac cgttcgcacg
     2641 gcgatgggca gcgtcgaacg tttcgaccag gtcgtcatcg cctgtcacag cgatcaggcc
     2701 ctgaagttgc tgcaggatgc ctcgccggca gaacaggaaa tcctcagcgc atttccgtac
     2761 caggagaacg aagccgttct ccacaccgac gagtcgctgc ttccgcgggc gccgctggca
     2821 cgcgcggcct ggaactacca cctgctcgcg gtccgcgaca acgcgatgcg cgaccgggtc
     2881 gccctcacct acgacatgaa cgtgctgcag tcgctcgatg cgccggtgcg tttcctggtg
     2941 acgctcaatc gcaccgccga catcgacccc gcgaaggtgc tgaagaccat cacctaccat
     3001 cacccggtct atctaccggc cggcgtggcg gcgcagaagc ggcgcgccga gatcagcggc
     3061 gtcaaccgca cgttttattg cggcgcctac tggcgttacg gctttcacga agacggcgtg
     3121 gtcagcgccg agtgggtgct cgaggagttc gcgcgcctgg cgcaatccgc gccgtttgaa
     3181 tctgcacgcc tggcccgcgc cgtataacct gccatgaaga gcgcgttgta ctcaggctgg
     3241 attcagcatc gccgcttcgg gcccgcccga aacagctttc gctatcgcat cttcatgagc
     3301 tacatcgacc tcgccgaact gcccggcctc ttcaagggac gctggttctg gtcggcacgc
     3361 cggccttcgc tcgcgtggtt ccgccgtcgc gatttcctgg gcccttcgca cctgccgctc
     3421 gactgcgccg tgcgcgatct cgtcgaacga cacaccggcg cgcggcccac cggccccatc
     3481 cgcctgctca cgcatctgcg ccacttcggc tacagcttca acccggtgag cttctattac
     3541 ctcttcgatg cgaacgacac gcgcgtcgaa accatcgtcg ccgagatcac caacacgccg
     3601 tggaaacagc ggcacgccta tgtgcttccc gtctccaccg ccgcgcatgg cgcgcgcacc
     3661 gcggcctggc gattcagctt cgagaaacag tttcacgtct cgccgttcat gcccatggac
     3721 gtgcgctacg agtggtgctt cggcgcgccc gcgcagggcc tgcacgtgca catggaaaac
     3781 tggcgtgctg gacaggcgga tttcgacgcc acgctgaatc tccatcgtga ggagatgacc
     3841 gcgggctcgc tggcgcgcgc gctcatccag tttccgctga tcaccgcaaa agtaagcgcg
     3901 ctcatctacg ggcaggccct gaagctgctg tggaagcgcg cgccattcca ctcccatccc
     3961 gacaaggttg aaaccgggtt tacgcatcca tgaacacacg ctcgccactg ctgctcgaga
     4021 gtctcgtctc aaccgggccg tcgaacccga cgctgctcgc acggctcggc cgcaaactgc
     4081 tgctcgggca actgcggcag atcgagcatg gcgaactgcg gctcgtggag cccgatggac
     4141 gccagcacgt gttcggcaag cgtcacgcgg cgttcgacct cggctgcacc gtctatttcg
     4201 acaatccgca gtcgttcgcg gacgcggcct tcggtggcac ggtgggcgcg ggtgaagcct
     4261 acatccgtgg tctgtggcga tgcgacgatc tcgtgacgct ggtgcgcatc ttcgtcgcca
     4321 accgcgagca gaccaatcgc atcgactcgc gctggtcgct cgtgagccgc ccgctgctcc
     4381 gcctgtttca ctgggccaat cgcaacagcc gtcacggcag cgcgcgcaat atcgccgcgc
     4441 attacgatct cggcaacgag ctctaccagc tgatgctcga tgacacgatg gcgtactcgt
     4501 gcgcgatctt tccgcatgcc ggcgccacac tggcggaagg ctccgaggcg aaattcgaag
     4561 cggtatgccg caagctcgac ctcaaaccga ctgatcacgt gctcgagatc ggcaccggct
     4621 ggggcggatt cgccatccat gccgttcaac gttatggctg ccgcgtcacc accaccacca
     4681 tttcgaagtc ccaggccgag ttcgccgtgc gcaagatcgc ggcgctgggc ctgtccgatc
     4741 gcatcaccgt attgctcgag gactatcgcg atctgaaagg cgatctcgcc ggtccctacg
     4801 acaagctcgt ctccatcgaa atggtcgagg ccgtcggcgc gcgcttcctc gacggctatt
     4861 tccgacactg ctcacggctg ctcaagcccg aaggcgcgat gctgctgcag tccatcacgc
     4921 tgcaggacca gttctacgac caggcgctga agtcggtcga cttcatccag cgctacgtgt
     4981 tcccgggcag cttcatcccc tcggtgcagg cactgtcgaa atcgctggcg aagtccaccg
     5041 atctgaaaat atttcatctc gaagacattg gccctcacta cgcccgcaca ttgcggctgt
     5101 ggcgcgagcg gtttttccga aacctgaata ccgtccgcga gctgggttac agcgagagct
     5161 tcatccgcct gtgggagtac tacctgtcct actgcgaagg cggcttcgaa gagcggcaga
     5221 tcggcaccgt gcagatgctg ctgaccaagc cccgctgccg ccggcagccg attcgatagc
     5281 accgggcggc ccggcggcgg cgcgcgttat gatgcgcgcg tgaacgacct ggctcattcc
     5341 ctgcacggcc tgcgggcgct ggtcgatgac gccacgatcc tcaccgatcc cgagcgcatc
     5401 gcaccgatgc tgcgcgacca tcgcgcgctg tatcgcggcc acgcgctggc cgtggtggcg
     5461 cccgccgata cggcgacggt gtcgcgcgtg ctcgcgttct gcaacgagca ccacatcggt
     5521 gtggttcccc agggcggcaa caccagctac tgcggcggcg cgacgccgga cgcgtccagc
     5581 agccaggttc tgctgtcgct ggcgcgcatg aatcgcgtgc gggccgtcga cgccaccaac
     5641 tacgccatga tcgccgaagc gggctgtgtg ctcgcgcatc tgcaagccgc ggccgccgaa
     5701 gcgggccgct atctaccctt gagtctgggc agcgaaggca gctgcatgct gggcggcaac
     5761 ctgtcgacca acgccggcgg gctcaacgtc atccactacg gcatggcgcg cgagctggtg
     5821 ctggggctcg aagtggtgct ggccgacggc cgcgtcatgg aaacactgac cgcactacgc
     5881 aaggacaaca ccggctacga catcaagtcg ctgttcctcg gcgcggaagg cacgctcggc
     5941 gt
//
LOCUS       DATDEU010000554         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2151283, whole genome shotgun sequence.
ACCESSION   DATDEU010000554 DATDEU010000000
VERSION     DATDEU010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151283"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1463)
                     /locus_tag="VIV63_13245"
     CDS             complement(24..1463)
                     /locus_tag="VIV63_13245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6125611.1"
                     /translation="MLSILVCHSYFLRFDQKQVQRAKPYPPLATIQVASLLRSAGHEV
                     SFFDSMLADDLERFERQLDVMRPQLVVFYEDNFNFLSKMCLGRMREACCDMIGLVRAR
                     GARLIAAGSDASDSPANYLSAGADAVLHGEGLGELVALVDRLNGDITLDAHTLAAGLS
                     HVSIAGATGPERSRAVAPLPEPRLASLPAWDLVDIEKYRRVWTKAHGRFSLNMAASRG
                     CSFRCHWCAKPIWGNQYVQRPPRETAAEMLYLRRHYRPDHIWFADDIFGFKADWVNEF
                     ATALAEGGGGVPFTIQLRADLVNARMAAALREARCQEVWIGAESGSQKILDAMNKGTS
                     VKQNLTARELLRTEGIRVGFFLQLGYLGEELDDILATRRLVDEARPDDVGVSVAYPLP
                     GTKFYEMVKEQLGDKRHWNESNDLEMMFHGTYTSDFYRVVRNLLHDQVTHPGSPELAN
                     RWNELIEREEEFRQDRPLALPGLMSLAHA"
     gene            1582..2214
                     /locus_tag="VIV63_13250"
     CDS             1582..2214
                     /locus_tag="VIV63_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY6125612.1"
                     /translation="MLGIHHYWLFIATAVLLVITPGQDTFFILGRSLSAGRSAGIAAA
                     LGITAGSVIHTFAAALGLSALLATSPYAFTVVKFAGAAYLIYIGVRALLTRANGLPGA
                     PGKDEADDRWSAFRQGVLSNLLNPKVALFFLALMPQFIAADSPDKVVAFLALGLTFVT
                     LGVIWCVVLAVAAAKLRGAFLRRPSMANVLNKVAGAMFIGLGLKLATARQ"
     gene            2211..>2504
                     /locus_tag="VIV63_13255"
     CDS             2211..>2504
                     /locus_tag="VIV63_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125613.1"
                     /translation="MSYQLKDQFGPSAPRAIAGMIRAVHPDFPHDDFLREVLAGYGPL
                     SLTGRGFHVAAALRKHLPAHFPQAVDVLLASASQPHGHQASGGMAAFLYMPHLF"
BASE COUNT          384 a          817 c          830 g          473 t
ORIGIN      
        1 aatagccgtg tgtgagcagg acgtcaggca tgggcgaggc tcatgaggcc gggcagggcc
       61 agcggacggt cctggcggaa ttcctcttcg cgctcgatga gctcgttcca gcggttcgcg
      121 agttcgggag aacccgggtg cgtgacctgg tcatgcagca ggttgcgcac cacccgatag
      181 aaatccgagg tgtaggtgcc gtggaacatc atctcgaggt cgttgctctc gttccagtgg
      241 cgcttgtcgc cgagctgctc cttgaccatc tcgtagaatt tcgtgcccgg cagtggatag
      301 gcgacgctga cgccgacgtc gtcgggccgt gcttcgtcga ccaggcgccg cgtggcgaga
      361 atgtcgtcca gttcttcgcc gaggtatccg agctgcagga agaagccgac gcgaattcct
      421 tccgtgcgca gcagttcacg ggccgtcagg ttctgcttca cgctcgtgcc cttgttcatg
      481 gcgtcgagga tcttctggct gccgctctcc gcgccgatcc agacttcctg gcaacgggct
      541 tcgcgcagcg cggccgccat gcgtgcgttc acgagatcgg cgcgcaactg gatggtgaag
      601 ggcacgccgc cgccgccttc ggccagcgcc gtcgcgaact cgttcaccca gtccgccttg
      661 aatccgaaga tgtcgtcggc gaaccagatg tggtcgggac ggtagtgacg gcgcaggtac
      721 agcatttccg ccgcggtttc gcgcggcggg cgctgcacgt actgattgcc ccagatgggc
      781 ttggcgcacc agtggcagcg gaaggagcaa ccgcgcgagg cggccatgtt gaggctgaag
      841 cggccgtggg ctttcgtcca cacgcgccgg tatttttcga tgtcgaccag gtcccacgcg
      901 ggtagtgagg cgagcctggg ttcgggcagc ggggcgaccg cgcgcgatct ttcggggccg
      961 gtggcgccgg cgatgcttac gtgggacagg cccgcggcga gcgtgtgcgc atcgagcgtg
     1021 atgtcgccgt tcaaccggtc gacgagcgcc acgagctcgc caagtccctc gccgtgcagc
     1081 acggcgtccg cgcccgcgct caagtagttg gccggcgaat ccgaggcatc ggagcccgcg
     1141 gcgatgaggc gcgcgccacg cgcacgcacc agcccgatca tgtcgcaaca cgcctcgcgc
     1201 atgcggccca ggcacatctt gctcaggaaa ttgaagttgt cctcgtagaa caccacgagt
     1261 tgcggacgca tgacgtcgag ctgccgctcg aagcgttcga ggtcgtccgc cagcatggag
     1321 tcgaagaacg acacctcgtg acccgcgctg cgcagcagcg aagccacctg gatcgtggca
     1381 agcggcggat acggcttggc gcgctgcacc tgcttctggt caaaacgcag aaaatacgaa
     1441 tggcaaacca gtattgagag catcgagtcc cttccccgcc cagacgagtt ggccttcgaa
     1501 cgtgagtgtc ccgggcctcc gatccggttc cccccccacg tttatttgcg ggccgggacg
     1561 gcacgcggca cagtgcggcc catgctcggc attcatcatt actggctgtt catcgccacc
     1621 gccgtcctgc tggtcatcac gcccggacag gacacgtttt tcatcctcgg ccgcagcctt
     1681 tcggctggcc ggtccgccgg cattgccgcc gcgttgggga tcaccgccgg cagcgtcatc
     1741 cacactttcg cagcggcgct gggattgtcg gcgttgctgg ccacgtcgcc gtacgccttc
     1801 accgtcgtga agttcgcggg cgcggcctat ctcatctaca tcggcgtccg cgcactgttg
     1861 acgcgggcga atggattgcc gggcgcgccg gggaaagacg aagccgacga ccggtggtcc
     1921 gcattccgcc agggagttct gtcgaatttg ttgaatccca aagtcgcgct gttcttcctg
     1981 gcgctgatgc cgcaattcat cgccgccgac agtccggaca aggtcgtcgc gttcctggcg
     2041 ctcggactca cgttcgtgac gctcggcgtg atctggtgcg tggtgctggc cgtggccgcc
     2101 gcgaaattac gcggcgcgtt cctgcgccgt ccgtcgatgg cgaatgtgct caacaaggtt
     2161 gccggcgcga tgttcatcgg gctcggcctg aagctggcca ccgcgcgtca gtgagctacc
     2221 agctcaagga ccagttcggc ccgagcgcgc cgcgcgccat cgcgggcatg atccgcgcgg
     2281 tccaccccga ttttccgcac gacgatttcc tgcgcgaggt gctcgcgggt tatgggccgt
     2341 tgtcgctcac gggccgcggg tttcacgtgg ccgcggcgct tcgcaaacac cttccggcgc
     2401 actttccgca ggcggtcgac gtgttgctgg cgtcggcgtc gcagccacac ggccaccagg
     2461 cttcgggcgg catggctgcg tttctctaca tgccccacct gttt
//
LOCUS       DATDEU010000555         3835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_692812, whole genome shotgun sequence.
ACCESSION   DATDEU010000555 DATDEU010000000
VERSION     DATDEU010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692812"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="VIV63_13260"
     CDS             complement(<1..1231)
                     /locus_tag="VIV63_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125614.1"
                     /translation="MGEQEKLQKYMPAVVDYRQRLAALQGGWDSLALLTHFGDGGADL
                     GNTRQAFDSLATELESHLSNETHKKAAQAAGSRAQVAIDIMVRNLFERTADVGFLAAD
                     DDLKHFAREASLLRRQVRDGDAAVASARLGSLTTQMQRRLAEYVAKYSVYSNVVLVSP
                     ASEVLVQLEGGSAPALTRDPLVARTLAAGNTYVETFGASDLVPGARRALIYSQQVAEG
                     GEVLGVLCLCFALEGECAGIFERLLGAQDWTVLALLDEHHEVIASSDAQQLPAGVRMR
                     SCEQSASVMRFAGREFLAIARGARPYQGYAGPPWRGYAMVPLQHAFESEGASGTTSAG
                     ALAGANLDSKAFSEGLLAVPLHAEAIQRDLSRSVWNGNVRLSTQDAANANFARALLRE
                     VGNLGRRTQEVFERSIED"
     gene            complement(1337..2197)
                     /locus_tag="VIV63_13265"
     CDS             complement(1337..2197)
                     /locus_tag="VIV63_13265"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY6125615.1"
                     /translation="MKNKLAIAFLLFSPLSIQAADKPGEWKALLDPAFSQFDVYLSYR
                     GDQIMSVIQGKAPPDLKPVGLNPAGQNVFTMIEQGGKPVLRISGEFYGCATTRQEFAN
                     YHFRASFKWGEKKWEPRLTELKDSGILYHSRGDFGVDYWKSWALSQEFQVIEHGIGEY
                     WRIASSEIDIRADPKAPGAVAPKWNPQAPWMAFAGSNNNALAGSDEDRPGEWNTLELV
                     CFEDRCVHIANGKVVMALKNARYKDGDKVLPMKGGKLQIQSEAAEVFYRDLEIRTIPA
                     MPKEYQRFFQ"
     gene            complement(2249..3469)
                     /locus_tag="VIV63_13270"
     CDS             complement(2249..3469)
                     /locus_tag="VIV63_13270"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY6125616.1"
                     /translation="MKSRKISTVMGLLLSVAMAVAARADAVMDWNARAEVIAADKRLA
                     PPVQARALALMHVSMFEAINAIERRYQPYRLDLVADRNTSREAAAASAAHAVLVELFA
                     DQKPALDAQLATELAPIAQGPAKERGILVGRKAAAELLALHADEGKGVDDYRPFTQAG
                     VYVPTQPVLGYHVGTFKPWVMESAKQFRAPPPPALTSDTWTRDVNEIRELGGLKSTAR
                     TDEQTTVGKFWFLTGARTYNPIVRQVVLAKQMNLLDCARVYALTSIAAADSFIAVFEA
                     KYHYHFWRPVTAIRNADLSQNPATPRDPSWQPLGDTPMHPEYPCAHCISSAAVAATLR
                     ALVGDDVGEISVTSNTAPGVTRRWTRLSDYANEVSNARIWSGFHYRFSTEAAQDMGRK
                     IGELTVATQLRPRK"
     gene            complement(3466..>3835)
                     /locus_tag="VIV63_13275"
     CDS             complement(3466..>3835)
                     /locus_tag="VIV63_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125617.1"
                     /translation="NPATAGDPSWQSLINNPNYPDYTSGANNVTAAMTRTLEWFFGTD
                     RMSFEVSSLAPLAVQKVRRYQRFSDAARDVVDARVYLGIHFRFADVAARNQGRRVADW
                     AFNHFLLPVGGREHHGHDQW"
BASE COUNT          647 a         1250 c         1232 g          706 t
ORIGIN      
        1 ggtcttcgat cgagcgctcg aacacttcct gcgtccggcg accgaggttg cccacttcgc
       61 gcagcaacgc gcgcgcgaag ttcgcgttcg ccgcgtcctg ggtagatagc cggacgttcc
      121 cgttccagac cgaacggctg agatcgcgct ggatggcttc ggcgtggagc ggcactgcga
      181 gcaatccttc ggaaaacgcc ttcgagtcca ggttcgcacc cgcgagcgcg ccggcgctgg
      241 tcgtgccaga agctccttcc gattcgaacg catgctgcag cggcaccatg gcgtacccgc
      301 gccacggtgg gccggcatat ccctgatagg gcctggcgcc gcgcgcgatg gcgagaaatt
      361 cacggccggc aaaccgcatg acgctggccg actgctcgca ggagcgcatg cgaacacccg
      421 ccggcaactg ctgggcgtcg ctgctcgcga tgacttcgtg gtgttcgtcg agcagcgcca
      481 gcaccgtcca gtcctgcgcg ccaagcagcc gctcgaagat gcccgcacac tcgccttcca
      541 gcgcgaaaca caggcacaac acgccgagca cttcgccgcc ttcggcaacc tgctgcgagt
      601 agatgagcgc gcggcgtgcg ccgggaacga gatcgctggc gccgaaagtt tcgacgtagg
      661 tattgccggc cgcgagcgta cgggcgacga gtgggtcgcg ggtcagcgcc ggagcgcttc
      721 cgccctcgag ctgcaccagg acttcggagg ccggcgatac gagcacgacg ttgctgtaca
      781 cggaatattt ggcgacgtat tcggcgagcc gccgctgcat ctgcgtggtg aggcttccga
      841 gccgggcgct ggccaccgca gcatcgccat cgcgaacctg ccggcgcagc aacgaagctt
      901 cacgcgcgaa atgtttgaga tcgtcgtccg cggcgagaaa gcccacgtcg gccgtgcgct
      961 cgaagaggtt gcggaccatg atgtcgatgg cgacctgggc acgcgacccg gcggcctgcg
     1021 cggccttctt gtgagtctcg ttcgacaggt gcgattcaag ttcggtcgcc agcgagtcga
     1081 acgcctgccg cgtgttgccg agatcggcgc cgccatcgcc aaagtgcgtg agcagcgcga
     1141 ggctgtccca gccgccctgc aatgcggcaa gccgctgtcg atagtccacg acagctggca
     1201 tgtatttctg cagcttttcc tgctcgccca cgatttttcc ggccgactcc tgacgaccct
     1261 tacttaacga ccccggggag catttcttga ttcgcggcgg cgatctgccg cgctcacgcg
     1321 acggcggtca cactattcac tggaaaaacc gctggtattc cttcggcatg gccggaatgg
     1381 tgcggatttc gaggtcgcgg taaaacacct ccgccgcctc gctttgaatc tgcagcttgc
     1441 cgcccttcat cggcagcacc ttgtcgccat ccttgtaacg cgcgttcttc agcgccatga
     1501 ccaccttgcc attggcgatg tgcacgcacc ggtcttcgaa acacaccagt tcgagcgtgt
     1561 tccattcgcc cggccggtct tcgtccgaac ccgcgagcgc attgttgttg gacccggcga
     1621 acgccatcca cggcgcctgg ggattccatt tcggcgccac ggcgcccggt gctttcggat
     1681 ccgcgcggat gtcgatctcg gagctcgcga tccgccaata ttcgccgatg ccgtgttcga
     1741 tgacctggaa ctcctgcgac aaggcccagg acttccagta gtcgacgccg aagtcgcccc
     1801 ggctgtgata caggattccg gaatccttga gctcggtcag cctcggctcc catttcttct
     1861 cgccccactt gaagctggcg cggaagtggt agttagcgaa ctcctggcgc gtggtggcgc
     1921 agccgtagaa ttcgccgctg atgcgcagca ccggcttgcc gccctgctcg atcatcgtga
     1981 acacgttctg gcccgcggga ttgaggccca ccggtttgag atccggcggc gccttgccct
     2041 ggatcacact catgatctgg tcgccccgat agctcaggta gacatcgaac tgcgagaacg
     2101 ccgggtcgag cagcgccttc cactcaccgg gtttgtcggc ggcctgaatg ctgagcgggc
     2161 tgaagaggag aaacgcgatc gccagtttgt ttttcatggg cggcgagtac acgtgagtcc
     2221 ccgccgccgg tcaagaaaag tccggctgct atttgcgcgg gcgcagttgc gtcgccacgg
     2281 tgagttcgcc gattttccga cccatgtcct gggccgcttc ggtcgagaac cgataatgga
     2341 atccggacca gatacgcgcg ttggacactt cgttggcgta atcggagagc cgcgtccacc
     2401 gacgcgtgac gcccggtgcc gtgttgctgg tcaccgatat ctcgcccacg tcgtcgccga
     2461 cgagcgcgcg caacgtggcc gccacggccg ccgagctgat gcaatgcgca cagggatatt
     2521 cggggtgcat gggcgtatcg ccgagcggct gccacgaagg atcgcgcggc gtggctggat
     2581 tctgtgacag gtccgcgttg cggatcgcgg tcacgggccg ccagaagtgg tagtgatatt
     2641 tcgcttcgaa cacggcgatg aaggaatcgg ccgccgcgat ggacgtcaag gcgtagacac
     2701 gcgcgcaatc gagcaggttc atctgcttcg ccaggaccac ctgccgcacg atggggttat
     2761 aggttcgcgc cccggtgagg aaccagaact tgccgacggt cgtctgctcg tcggtgcgtg
     2821 cagtgctctt caagccaccg agctcgcgaa tctcgttcac gtcgcgagtc catgtatccg
     2881 acgtcagcgc cggtggcggt ggcgcccgaa actgcttggc ggattccatg acccagggct
     2941 tgaacgtccc gacgtgatat cccagcacgg gctgcgtggg cacatagacc cccgcctgcg
     3001 tgaacggccg gtagtcatcg acgcctttgc cctcgtcggc gtgcagggcg agcagttcgg
     3061 ccgcggcctt gcggccgacc agaattccac gttcctttgc cgggccttgc gcaatcgggg
     3121 ccagctccgt ggcgagctgc gcatcgagcg ccggcttctg atcggcgaac agctccacca
     3181 gcaccgcatg ggctgcactc gccgccgcgg cttcgcgcga ggtgttgcgg tcggcgacga
     3241 ggtccaggcg gtacggctgg tagcgccgct cgatggcgtt gatggcctcg aacatcgaga
     3301 catgcatgag cgccagcgcc cgggcctgaa cgggcggtgc gaggcgtttg tcggcggcga
     3361 tgacctcggc cctggcgttc cagtccatga cggcatcggc acgtgctgcc acggccatcg
     3421 ctaccgacag cagcaacccc atgaccgtgc tgatcttgcg cgatttcacc attgatcatg
     3481 tccgtgatgt tcacggcctc cgacgggcag cagaaaatga ttgaaggccc agtctgccac
     3541 gcgccgtccc tggttgcgcg ccgccacgtc ggcgaagcgg aagtggatgc ccaggtacac
     3601 gcgggcgtcg acgacatcgc gcgccgcatc cgaaaaccgc tggtacctgc ggaccttctg
     3661 cacggccagc ggagcgagac tcgagacctc gaaagacatc ctgtccgtgc cgaagaacca
     3721 ttcgagcgtg cgcgtcatgg cagcggtcac gttgttcgcc ccggaggtat agtccgggta
     3781 gttaggattg ttgatcaacg actgccacga cggatcaccg gcggtcgcgg ggttc
//
LOCUS       DATDEU010000556         4892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2005736, whole genome shotgun sequence.
ACCESSION   DATDEU010000556 DATDEU010000000
VERSION     DATDEU010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005736"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..919
                     /gene="pyrH"
                     /locus_tag="VIV63_13280"
     CDS             200..919
                     /gene="pyrH"
                     /locus_tag="VIV63_13280"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY6125618.1"
                     /translation="MTAAPYKRILVKLSGEALLGKTEFGIDPAVLKRIAGEVLEIQRS
                     GVQVACVIGGGNIFRGSGLAKAGIDRATADHMGMLATVMNALAMQDAIEGLGGNARVQ
                     SAIRINQVCEDYIRRRAMRHLEKGRIVIFAAGTGNPYFTTDSAAALRAVEIEADVLLK
                     ATKVNGIYDSDPMKNPDAKRYATLTYDKVLQDRLNVMDATAIVMCRDNNMPLQVFNLN
                     NAGDLPRVVRGEDIGTVVTNV"
     gene            945..1502
                     /gene="frr"
                     /locus_tag="VIV63_13285"
     CDS             945..1502
                     /gene="frr"
                     /locus_tag="VIV63_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY6125619.1"
                     /translation="MLDDIKKDAATRMGKCVENFQGDLKKLRTGRAHPSLIENMKVDY
                     YGTDTPLKQVANISVEDARTLVVSPWEKSMVQAIEKAIHKSDLGLNPMSAGTVIRIPL
                     PPLTEERRKDITKVARADAENARVAVRNVRRDVIADVKEALKEKLITQDDEKKAEGDI
                     QKLTDKYIADIETQLAAKEKELLQV"
     gene            1534..2265
                     /gene="uppS"
                     /locus_tag="VIV63_13290"
     CDS             1534..2265
                     /gene="uppS"
                     /locus_tag="VIV63_13290"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY6125620.1"
                     /translation="MSIPKHIAIIMDGNGRWAEARGLARHSGHKEGVRPVRLCIEECA
                     RRGVGALTLFAFSSENWQRPTIEVTSLMQLFLEAIDREVEDLHKNKVRLRFIGDRHAL
                     SVKLQARMAAGEELTAANPGLQLQVAVSYGGRWDIVQAAKRLAAQVASGAISLEQIDE
                     DRFASELQLGDIPDPDLFIRTGGDHRISNFLLWNLAYTELHVCETLWPDFGVADLDEA
                     LASFAGRERRFGLTRPQLQEATRSA"
     gene            2258..3064
                     /locus_tag="VIV63_13295"
     CDS             2258..3064
                     /locus_tag="VIV63_13295"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY6125621.1"
                     /translation="MLKERVVTAIVLAAVLLGVMLGLPPVATVWLLTVLVLVGAWEWA
                     GFIGKTTPATRAAFTIAIALALVACLYFYSVSSSFVSIVMTIAVAWWFIAFLWLCLSP
                     GRVTPVTAALAGFLSLVPCWLALVHVTFITQSTRWVLFTLALVWAADTGAFFAGRWLG
                     RVPLAPRVSPKKTWEGALGGVALSGLVAWASAKWVFDVDAWPFVMTCIAVAALSIVGD
                     LTESMLKRAAGLKDSGTLFPGHGGMLDRIDSVTAAAPALVFALIGLKVIA"
     gene            3061..4263
                     /locus_tag="VIV63_13300"
     CDS             3061..4263
                     /locus_tag="VIV63_13300"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY6125622.1"
                     /translation="MSSDGVKGVAILGSTGSVGEHTLDVIARHPDRFRVVALGANRNV
                     EKLADQCLRFNVPYAALGDSTATARLESELRARKAPTRVIGGADAMVTIAALPEVDSV
                     MAAIVGAAGLPSTLAAARAGKRLLLANKESLVMAGPLLMQIVRNAGATLLPIDSEHNA
                     VFQCLPHGTRAGVEPPGLKRILLTASGGPFLDVDSSRLERVTPDEACAHPNWVMGRKI
                     SVDSATLMNKGLEFIEACVLFGVKPSQVDVVIHRESIVHSLVEFVDGSMLAQLGAPDM
                     RTPIAHALAWPERVASGVQSLDLVKVGSLRFEAPDLRRFPALALAQEAARAGGLRPAS
                     LNAANEVAVSAFLSGGLNFARIPAVIESVMATTSGGEIRDLDDVLAADAEARVRASSF
                     IRPRAVHA"
     gene            4280..>4892
                     /gene="rseP"
                     /locus_tag="VIV63_13305"
     CDS             4280..>4892
                     /gene="rseP"
                     /locus_tag="VIV63_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY6125623.1"
                     /translation="MQIGWNILWFIVGVSLLVTVHEFGHFWVARKLGFKVLRFSVGFG
                     KPLYTKVGAAPDHTEFVIAALPLGGYVRMLDERDGAVSPEDLPRAFASKPPWQRILVM
                     LAGPAANILFAILILWVIFWTNGINHVKAVVDNVVVGSPAAAAGLANGDEIRSIDGDP
                     VLDQVDASLGLLDAISDDGEAVMTVRNRTGTERNVTLAIPDPDL"
BASE COUNT          872 a         1588 c         1588 g          844 t
ORIGIN      
        1 aagaagcagg gcgatttcgc gaacgaagtg atggagcagg tcaaagcggc ccaaaccaag
       61 actgaccctc cggcgaccaa gcactagccg ctacgaaaac cccgcctcta gcggggtttt
      121 ctttatggca aagtgcgcaa cgcgtcgggg gcccctcgaa gagggggcct ctggatttga
      181 gacaagggga atcgagcaaa tgaccgctgc gccgtacaaa cgcattctcg tcaagctttc
      241 gggcgaagcg ctgctcggca agaccgagtt cggcattgac ccggcggtac tcaagcgcat
      301 cgccggcgaa gtgctggaga tccagcgcag cggcgtgcaa gtggcgtgcg tgatcggcgg
      361 cggcaacatc ttccgcggct ctggcctcgc caaggccggc atcgaccgcg ccacggccga
      421 tcacatgggc atgctggcca cggtcatgaa tgcgctggcc atgcaggacg ccatcgaggg
      481 actcggcggc aatgcgcgcg tgcagagtgc gatccgcatc aaccaggtgt gcgaggacta
      541 cattcgccga cgtgccatgc gccacctcga gaagggacgc atcgtgatct tcgcggcggg
      601 caccggcaat ccatatttca cgaccgacag cgccgcggct ctgcgcgccg tcgagatcga
      661 ggcggacgtg ctgctcaagg ccaccaaggt caatggcatc tacgactccg acccgatgaa
      721 gaacccggat gccaagcgct acgccacgct cacctacgac aaggtgttgc aggaccgcct
      781 gaacgtcatg gatgccacgg ccatcgtgat gtgccgcgac aacaacatgc cgctgcaggt
      841 gttcaacctc aacaatgcgg gcgacctgcc gcgcgtggtg cgtggcgagg acatcggcac
      901 agtcgtcacc aacgtttgac gtttacgttc gagggcaagg aatcatgtta gacgacatca
      961 agaaagacgc agccacccgc atgggcaagt gcgtcgagaa tttccagggc gatctcaaga
     1021 agctgcgcac cggccgcgcg catccgtcgc tcatcgagaa catgaaggtg gactactacg
     1081 gcacggatac gccgctcaag caggtggcca acatcagcgt cgaagacgcg cgcacgctgg
     1141 tcgtgtcgcc gtgggagaag tcgatggtcc aggccatcga gaaggcgatt cacaagtcgg
     1201 atcttggcct gaacccgatg agcgcgggca cggtcatccg catcccgctg ccgccactga
     1261 ccgaagagcg ccgcaaggac atcaccaagg tggctcgcgc cgatgccgag aacgcgcgcg
     1321 tcgccgtgcg caacgtacgc cgcgacgtga tcgccgacgt gaaggaagcg ctcaaggaaa
     1381 agctcatcac gcaggacgac gagaagaagg ccgagggcga catccagaag ctgaccgaca
     1441 agtacatcgc cgacatcgaa acccagctcg ccgccaagga aaaagaactg ctgcaggtgt
     1501 gagcgggcgc cgctcgactc gttgctgccc accgtgagca ttcccaaaca catcgccatc
     1561 atcatggacg gcaacggaag gtgggccgag gcgcgcggac tcgcgcgtca ttccggccac
     1621 aaggaaggtg tgcgtccggt ccgcctgtgc atcgaggagt gcgcgcgtcg cggtgtcggc
     1681 gcgctcacgc tgttcgcctt ctcgagcgag aactggcagc ggcccacgat cgaagtgacc
     1741 agcctgatgc agttgttcct cgaggcgatc gatcgcgagg tcgaggacct gcacaagaac
     1801 aaggtccggc tgcgtttcat cggcgatcgt cacgcgctca gcgtcaaact gcaggcgcgc
     1861 atggccgcag gcgaagaact caccgcggcg aatccgggac ttcaattgca ggtggccgtg
     1921 agttacggcg gccgttggga catcgtgcag gccgcgaaac gtctcgcggc gcaggtcgcg
     1981 agcggcgcga tctcgctcga gcagatcgac gaagaccgtt tcgcgagcga acttcagctc
     2041 ggcgacattc ccgatccgga cctcttcatt cgcaccggcg gcgatcaccg catcagcaac
     2101 ttcctgttgt ggaatctcgc gtacacggag ttgcacgtct gcgaaacgct gtggcctgac
     2161 ttcggcgtcg cggatctcga cgaggcgctc gcctcgtttg ccggccgcga gcgccgcttc
     2221 ggtctcaccc gcccgcaatt acaagaggcg acgcgcagtg cttaaggaac gcgtcgtcac
     2281 cgccatcgtg ctcgcggccg tattgctcgg cgtgatgctc gggcttcctc cggtcgccac
     2341 ggtatggctg ctcaccgtgc tcgtgttggt gggcgcctgg gaatgggccg gcttcattgg
     2401 caagaccacg ccggcaaccc gcgcggcttt cacgattgcc atcgcgctgg cgctcgtcgc
     2461 ctgcctctac ttctattctg tctccagcag cttcgtcagc attgtcatga ccatcgccgt
     2521 ggcgtggtgg ttcatcgcct tcctgtggct ctgcctgtcg cccggtcgag tcacgcccgt
     2581 gacggccgcg ctcgcgggat tcctgtcgct cgtgccttgc tggctggcgc tggtgcacgt
     2641 cacgttcatc acgcagagca cgcgctgggt gctgttcaca ctcgcgctgg tgtgggccgc
     2701 cgacaccggc gcgttcttcg ccgggcggtg gttaggccgt gtgccgctcg cgccgcgcgt
     2761 gtcgccgaag aagacctggg aaggcgcgct gggcggagtg gccctgagcg gcctggtcgc
     2821 ctgggcctcg gcgaagtggg tgttcgatgt ggacgcctgg ccgttcgtca tgacctgcat
     2881 cgcagtggcg gcgctctcca tcgtcggcga cctgacggaa agcatgttga agcgtgccgc
     2941 cggactcaag gacagcggca cgttgttccc cggtcatggc ggcatgctgg atcgcatcga
     3001 cagcgtcacg gctgccgcgc ccgcgctggt cttcgcgctc atcggcctca aggtcatcgc
     3061 atgagcagtg atggcgtgaa aggcgttgcc atcctgggct ccaccgggtc ggtgggcgag
     3121 cacacactcg atgtgatcgc gcggcatccc gatcgctttc gcgtcgtcgc gctgggcgcg
     3181 aatcgcaacg tcgaaaaact cgccgaccaa tgcctgcgtt tcaacgttcc ctacgcggcg
     3241 ctgggcgaca gcacggccac ggcccgcctc gaaagcgaac tgcgcgcgcg caaggcaccc
     3301 acgcgggtga tcggcggcgc ggacgccatg gtcacgatcg cggcgctgcc ggaagtggac
     3361 agcgtcatgg cggcgatagt cggggcggcg gggctgccgt cgacgctggc tgcggcgcgc
     3421 gccggcaagc ggctgttgtt agccaacaag gaatcgctgg tgatggcggg gccgctgctc
     3481 atgcagatcg tgcgcaacgc cggcgccacg ctgctgccca tcgacagcga acacaacgcc
     3541 gttttccagt gcctgccgca tggcacgcga gccggtgtcg agcctccggg cctgaagcga
     3601 atcctgctga ccgcctccgg cggacccttc ctcgacgtgg atagttcgcg cctcgagcgt
     3661 gtgacgcccg acgaagcctg tgcccatccg aactgggtga tgggccggaa gatctcggtg
     3721 gactccgcca cgctcatgaa caagggcctc gaattcatcg aagcctgcgt gctgttcggc
     3781 gtgaaaccct cccaggtcga tgtggtcatt caccgcgaga gcatcgtcca ttcactggtc
     3841 gagttcgtcg atggctcgat gctcgcgcaa ctgggcgccc cggacatgcg cacgcccatt
     3901 gcgcatgcgc tcgcgtggcc ggagcgagtg gcctcgggtg tacaatcgct cgacctcgtg
     3961 aaggtgggat ccctgcgatt cgaggcgccg gacctgcggc gctttccggc gctggccctg
     4021 gcccaggagg ccgcccgggc gggtggcctg cgccccgcat cacttaatgc cgccaacgag
     4081 gtggccgtat cggcctttct cagcggtgga ttgaactttg cacgaattcc ggcagtcatt
     4141 gagtcagtga tggcaaccac atcgggaggc gaaattcgcg atctggacga tgtgctggcg
     4201 gccgacgccg aagcgcgagt ccgcgcttcc tcattcatcc ggcctcgcgc cgtgcacgcc
     4261 taaggctcag ggtcactcga tgcagatcgg ctggaacatt ctctggttca tcgtgggcgt
     4321 cagcctgctg gtcaccgtgc acgagttcgg gcacttctgg gtggcccgca aactcgggtt
     4381 caaggtgctg cgcttctcgg tgggtttcgg caagccgctc tacaccaagg tcggtgcggc
     4441 gcctgaccac acggaattcg tcattgccgc gctgccactc ggcggatacg tgcgcatgct
     4501 cgacgagcgc gatggcgctg tctccccgga agatctgccg cgggctttcg ccagcaagcc
     4561 gccgtggcaa cgcatcctcg tcatgctcgc cggtcccgcg gcgaacatcc tgttcgccat
     4621 cctcatcctc tgggtcatct tctggacgaa cggcatcaac cacgtgaaag ccgtggtcga
     4681 caacgtggtc gtcgggtcgc ccgccgccgc cgccggactc gccaacggcg atgaaatccg
     4741 cagcatcgac ggcgatccgg tactcgacca ggtcgacgcc tcgctcgggt tgttagacgc
     4801 gatcagcgac gacggcgaag ccgtcatgac ggtgcgcaat cgcaccggca ccgaacgcaa
     4861 tgtcaccctc gcgatccccg atccggatct gc
//
LOCUS       DATDEU010000557         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_984967, whole genome shotgun sequence.
ACCESSION   DATDEU010000557 DATDEU010000000
VERSION     DATDEU010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984967"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /gene="motA"
                     /locus_tag="VIV63_13310"
     CDS             complement(<1..852)
                     /gene="motA"
                     /locus_tag="VIV63_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEY6125624.1"
                     /translation="MIQVLGSIIVLACVLGGFVLEGGHLLALWHPTEILIIVGAALGA
                     FLTSNPPKVSKQAFVLALTLPKGARYKRDDYVTLLKLIYDILMKIRKDGLLAIESDIE
                     QPEKSALFQKYPVILDDHHMVVFITDCLRLITGGSLDPHELESLLEYELETHHKEAHE
                     PCHAVQKVADALPGFGIVAAVLGIVNTMSALEGADTATIGHKVGAALVGTFLGILVAY
                     GFVGPIASAMEHRANEESKAFEVVKMALVASVRGYAPTVAVEFARKLLFSEVRPTFAD
                     LEAHLKGK"
     gene            1051..>2204
                     /locus_tag="VIV63_13315"
     CDS             1051..>2204
                     /locus_tag="VIV63_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125625.1"
                     /translation="MSNRVGFLSIVCLFSLAAQAADEPQWLKDARAREGKTGKALELK
                     SKDKWFSAKVPAKVIGDIEKVEGSYTVELNIGSEQSAYCEVMPDGFDMADMVRRNLEF
                     TMEQVAKTQGTVELRHLEAIDAGVFGNVPYLQTQWIYRVNDKGEQRLGGLKQISMVKD
                     DKGIYCAHVDLGYNKTFAAVARAFADTFESTTPGPKPYYEEISVATMNGMKIGIALST
                     LVRDAEGDTEATQTTSLLAPLPQGAMHSQDGVHQEWIRPDSSMINATHFVASNGELTT
                     SLGLKIKDDAWIIEGDHDGKKIAETLKADAQPGTWVAQAFAIRKLLATPNPVGAEHTM
                     PQWMTDNPTKLSDAKTKVLGKAGDKQYKAFVTAGEMNANVTIETATGMIDV"
BASE COUNT          468 a          679 c          675 g          382 t
ORIGIN      
        1 cttgcccttg aggtgcgctt cgaggtcggc gaatgtcggc cgcacttcgc tgaacagcag
       61 cttgcgcgca aactcgaccg cgacggtcgg cgcatagccg cgcacgctcg cgaccagtgc
      121 catcttcacc acttcgaaag ccttgctctc ttcgttcgcg cgatgctcca tcgccgaagc
      181 gatgggaccc acgaatccgt aggcaacgag aatgccgagg aaggtgccca cgagcgccgc
      241 gccgaccttg tgaccgatgg tcgctgtgtc cgcgccttcg agcgccgaca tggtgttcac
      301 gatgccgagc accgccgcca cgatgccgaa gccgggcagc gcgtcggcca ccttctgcac
      361 ggcgtgacag ggttcgtgcg cttccttgtg atgggtctcg agttcgtact cgagcaggct
      421 ctcgagctcg tgcggatcga gcgagccgcc cgtgatcagg cgcaggcagt cggtgatgaa
      481 caccaccatg tggtgatcgt cgaggatcac cggatacttc tggaacagcg cactcttctc
      541 gggctgctcg atgtcacttt cgatggccag gagaccgtct ttacggatct tcatcagaat
      601 gtcgtagatg agcttgagca gcgtgacgta gtcgtcacgc ttgtaccgcg cgcccttggg
      661 cagcgtgagc gcgagcacga acgcctgctt ggaaactttc ggcggattgc tggtcaggaa
      721 cgcgccgaga gccgcaccca cgatgatcag gatttccgtc gggtgccaca gcgcgagcag
      781 atgtccgccc tcgagcacga agccgccgag aacgcacgcg agaacgatga tggagcctaa
      841 gacctgaatc attctgataa gtccggagtg agcctgtgga cgcggtccgc ccgtaccgga
      901 tatcggtcga gtcatggctt ccttaagtcg aaccaaatca gggcggcaaa ggcctgccac
      961 cctgcgtgaa tgcgttaagg atttggcgtg cgcgcgccga ggccttacat ttctgtgacc
     1021 gggctccttg tccggcaacc gggaggttcg atgagcaatc gcgttggatt tttgtcgatc
     1081 gtgtgtctgt tcagccttgc cgcacaggcg gcggatgaac cccagtggct caaggacgcg
     1141 cgggcgcgcg agggcaaaac cggcaaggca ctcgagctca agagcaagga caaatggttt
     1201 tcggcgaaag tccccgcgaa ggtcatcggc gacatcgaga aggtcgaagg ctcgtacacc
     1261 gtggagctca atatcggcag cgagcaatcc gcctattgcg aagtcatgcc cgacgggttc
     1321 gacatggcgg acatggttcg ccgcaacctc gaattcacca tggaacaggt ggcgaaaacc
     1381 cagggtacgg tcgaactccg ccacctcgaa gccatcgacg ccggcgtctt cggcaacgta
     1441 ccctatctac agacgcagtg gatttatcgc gtgaacgaca agggcgaaca gcgtctcggc
     1501 ggcctgaagc agatttccat ggtgaaggac gacaagggca tttactgcgc gcacgtcgac
     1561 ctcgggtaca acaagacgtt cgccgcggtc gcccgcgcgt tcgccgacac gttcgagtcc
     1621 acgactcccg gaccgaagcc gtactacgag gaaatctcgg tagcgaccat gaacggcatg
     1681 aagatcggaa tcgcgctctc cacgctggtc cgcgacgccg agggcgacac cgaggccacg
     1741 caaaccacat cgctgctcgc cccgcttccc cagggcgcga tgcattcgca ggacggcgtt
     1801 caccaggaat ggattcggcc cgacagctcc atgatcaatg cgacgcattt cgtcgcgtcc
     1861 aacggtgaac tcaccaccag tctcgggctc aagatcaagg acgacgcctg gatcatcgag
     1921 ggcgatcatg acggcaagaa gatcgccgag acgctgaagg ccgatgccca gccgggcacc
     1981 tgggtcgcgc aggctttcgc gatccgcaaa ctgctggcca cgcccaaccc cgttggcgcc
     2041 gagcacacca tgccgcaatg gatgactgac aatcccacga aactcagcga tgccaagacc
     2101 aaggtgctgg gcaaagcggg tgacaaacag tacaaggcgt tcgtcactgc aggagaaatg
     2161 aacgccaacg tgaccatcga gacggccacc gggatgatcg atgt
//
LOCUS       DATDEU010000558         4340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1276743, whole genome shotgun sequence.
ACCESSION   DATDEU010000558 DATDEU010000000
VERSION     DATDEU010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276743"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1313
                     /gene="bamB"
                     /locus_tag="VIV63_13320"
     CDS             114..1313
                     /gene="bamB"
                     /locus_tag="VIV63_13320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEY6125626.1"
                     /translation="MKQLSLVFLATLLVLSACDSKDKKVEKPAELTDIKNPTVRIQKV
                     WGASVGGGGKKLRLGLGLATAGNRLFAAGRDGDVAAFDLKTGKQVWRVETKLALAGGT
                     GVGADIVAVGSADGQVLAFAADTGAQRWHADVKGEILSAPAVADSEVVVRTVDGKLRG
                     LAIADGKELWTAEQQIPRLTLRGAAAPVVARDVAISGFDNGRVLAVSLADGATVWDSP
                     VSPAHGRTELERLNDIDAAVKVVGDEVFVAGFQGRAAMLALDSGQIWWTRDVSSYRGV
                     EVDDDQMYVSTSQGEVVALKRRSGIEVWRNDLLLRRSLSAPAVVGDFVVVADLEGYVH
                     WFDRITGTLAGRVKSGGDRVTNPPLSVEGTLYVITDKGDIVALRGTPVASQAAKAEPA
                     PAPAPGG"
     gene            1362..2831
                     /gene="der"
                     /locus_tag="VIV63_13325"
                     /pseudo
     CDS             1362..2831
                     /gene="der"
                     /locus_tag="VIV63_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
     gene            3239..>4340
                     /locus_tag="VIV63_13330"
     CDS             3239..>4340
                     /locus_tag="VIV63_13330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY6125627.1"
                     /translation="MIGLMLCAPVAAMAADAGFTDRIIVKYRNSSAVASAAAQDSQMR
                     GTDMSAARFGVAMSRVRTTALGSQVLKVDRRLSVTEAEQLSRDIVASDPNVEYAEPDR
                     IMRRTLTPNDTRYNEQWHYFEATGGINAPPAWDKATGSGVVVAVIDTGYRPHADLAAN
                     LLPGYDFIGDTFVANDGNGRDSDASDPGDWLNAGECGGGDPATFEASSWHGTHVAGTI
                     AAVTNNSNGVAGVAFGAKVVPARVLGKCGGYTSDIADAIVWTSGGTVTGVPANANPAK
                     VLSISLGGGGACDSTTQSAINSARSRGANVIVAAGNSNANSANFSPANCSGVVTVAAV
                     GRNGGKASYSNFGATVEVAAPGGSGGANSVLST"
BASE COUNT          779 a         1435 c         1440 g          686 t
ORIGIN      
        1 gaatacctgg cggcccgttc ggcggagaac aacggccgca tcgacgtcga actgctcgac
       61 ctcaaaatcc gcgatctcgg cggaacgccg ccggccacgc ctggggaatc ctgatgaagc
      121 aattgagcct ggtcttcctc gccacgctgc tcgtgctgag cgcgtgcgac tcgaaagaca
      181 agaaggtcga aaaacccgcc gagctcacgg acatcaagaa tccaacggtc cgcatccaga
      241 aagtctgggg cgcgagcgtc ggcggtggtg gcaagaaact tcggttggga ctggggctgg
      301 ccacggccgg caacaggctg ttcgcggccg gccgtgacgg cgacgtcgcg gcgttcgacc
      361 tgaagaccgg caagcaggtc tggcgcgtcg agaccaagct cgcgctggcg ggcggcacgg
      421 gtgtcggcgc cgacatcgtc gccgtgggtt cggccgacgg ccaggtactc gctttcgcag
      481 ccgacaccgg cgcgcagcgc tggcatgccg acgtaaaggg cgaaattctt tccgctcccg
      541 ccgtggcgga cagcgaagtc gtcgtacgca ccgtcgacgg caaactgcgc ggtctcgcga
      601 tcgccgatgg caaggaactc tggaccgcgg aacagcagat tccgcgcctg acgctgcgtg
      661 gcgcggccgc gccggtcgtg gcgcgtgacg tggccatttc cggtttcgac aacggccggg
      721 tactggccgt cagtctggcc gacggcgcca ccgtgtggga ttcgccggtg tcgccggcgc
      781 atggccgcac cgaactcgag cggctcaacg acatcgacgc cgccgtgaaa gtggtgggcg
      841 acgaagtatt cgtcgcggga ttccagggcc gcgccgccat gctggcgctc gactcggggc
      901 agatctggtg gacgcgcgac gtgtccagct atcgcggcgt cgaagtcgac gacgaccaga
      961 tgtacgtttc cacgtcacag ggtgaagtcg tggcgctcaa gcgccgcagc ggcatcgaag
     1021 tgtggcgcaa cgacctgctg ctgcgtcgca gcctctccgc tccggcggtc gtgggcgatt
     1081 tcgtcgtggt cgcggatctc gagggctacg tgcactggtt cgaccggatc accgggacgc
     1141 tcgcgggtcg cgtgaagagt ggcggcgatc gcgtcaccaa tccgccgctg tcggtcgaag
     1201 gcactctcta cgtgatcacc gacaagggcg acatcgtcgc cttgcgcggc acgcccgtgg
     1261 cgagccaggc ggcgaaggcc gaacctgcgc ccgctcccgc gccgggtggc tgagcgcaag
     1321 ctcagtcacc aggctcaacc ttccacatgc ttccggtcat cgcgatcgtc ggacggccga
     1381 acgtcggcaa gtccacgctg ttcaacacgc tgacgcagac gcgcgacgcc atcgtcgccg
     1441 acgtgcccgg cgtcacgcgc gatcgccagt acggctacgg gaagttaggc ccggtgcagt
     1501 acgtctgcat cgacaccggc ggcctggtcg agaatccgag cggcatggac gcgctgatgc
     1561 gcatccagac cgagcatgcg atccgcgaag cggaccggct gatcttcgtc gccgacgcgc
     1621 gcgacggcct cacaccccag gatcaatttt ttgcgcgcga gttacgccgc gcgggcaaac
     1681 ccgtgtttct cgcggtcaac aaggccgaag gcctcgcggc cgggctcgcc ggcgccgatt
     1741 ttcacagcat gggactcggc gaaccgcatg cgatcgccgc gacgcacggg ttcggctgcg
     1801 aagacctcat ggacatcgtg ctggcgggct ttccgcccac gccatccgac gaccaggagc
     1861 ccgaagacgg gcgcatccgc atcgccgtca tcggccggcc caacgtcggc aagtcgacgc
     1921 tgatcaaccg cctcatcggc gatgagcggc tcatcaccag cgagatcgcc ggcaccacgc
     1981 gcgacagcat cctcgtgccg ttcgagcgcg acggccgcag cttcacgctg atcgacaccg
     2041 ccggcgtgcg ccgccgcgcg aagatcgatg acgaaatcga aatcctgtcg gtgtcgaaga
     2101 cactgcaggc catcgcggag gcgcatgtcg tcgtcatggt ggtcgatgcg caggattcca
     2161 tcggcgagca ggatgcgagc gtgctgggcc tggcgctgtc gcgcggccgc gcgctgatcc
     2221 tcgccgtgaa caaatgggac aacattccga tcgagaagcg tgacgacatc cgtcgtctgc
     2281 tctcgctcaa gctcgacttc gtgccgttcg cgcccatgca tttcgtgagt gcgcgccatg
     2341 gcacgggcgt gggcgacctc gtcgcgtcca tgatccgcgc ctacgaagcg gcgatgcgcc
     2401 agatgaagac gccgcagctg accaaggcca tggaaagggc gatggtgcag caccagccgc
     2461 ccatcgtgcg tggccggcgc atccgactgc gctacgcgca ccagggtggc cgaaatccgc
     2521 cgcgcatcat cgtgcacggc agccaggccg cgcacgtgcc cgagtcctac aagcgttatc
     2581 tcgcgaacat gttccgcgaa gagttcgacc tgttcgccac gcccgtgatg gtggacttcc
     2641 gcagcgacgt gaatccgttt ctgaaggaca agcctaagga aaagcgcggg ctcaagactt
     2701 cgcagaagga acggcgcgag cggcgcatca cgaaagaccg cgccaagcgt ccgcccaaga
     2761 agaaaggcgg cggctcgaag cagcccggcg caccccgcgg ccgcgccaat cggccggtca
     2821 acaagaagta gttcgggtag gtagcgctta catcgcgctc cactatcttc cgggcgcgcg
     2881 cgagtactcc tccgcgcacg aacgcgcaag cgtgacaccg tgtcagacgc cattcgccga
     2941 caaattttcg cgtcgcttga attgttcgcc ccttcgtcct agttttggct ccacgcgcag
     3001 gtcgaccatg gaaggtcttt cgcgacccca gggcagattc tgcagaagtt agttgagcgt
     3061 cacggctcga acgccgcagg cgccgaactt aggagcagtg caggatgcac ctttgcaacg
     3121 cgcagattcg cgtcggactt ttttggtccg cgttttcatc gctcaactcg cttttgcggg
     3181 atctcccagt caaaccaatc gtcaagggag atttatgact cgcctaacca aaaggataat
     3241 gatcgggctg atgttgtgcg caccggtggc ggccatggct gccgatgcag gcttcaccga
     3301 tcgaatcatc gtcaagtatc gaaactcatc cgcggtcgcg tccgccgcgg cgcaggactc
     3361 gcagatgcgc ggcacggaca tgtccgcggc gcgtttcggc gtggccatga gccgggtgcg
     3421 caccacggcg ctcggttcgc aggtgctcaa ggtcgaccgg cgtctctcgg tcaccgaggc
     3481 cgagcagctg tcgcgtgaca tcgtcgccag cgatccgaac gtcgagtacg ccgagcccga
     3541 tcgcatcatg cgcaggacac tgacgcccaa cgacacgcgc tacaacgagc agtggcatta
     3601 cttcgaagcc acgggcggca tcaacgcgcc acccgcgtgg gacaaggcca cgggcagcgg
     3661 cgtggtggtg gcggtgatcg acaccggcta ccggccgcat gccgatctcg cggccaacct
     3721 cctgccgggc tacgacttca ttggtgacac cttcgtcgcc aacgacggca acggccgcga
     3781 cagcgacgcc agcgatccgg gcgactggct caatgcgggc gagtgcggcg gcggtgatcc
     3841 cgcgactttc gaagccagca gctggcatgg cacacacgtg gcgggaacca tcgccgcggt
     3901 gaccaacaat tcgaacggcg tggccggcgt ggccttcggc gccaaggtgg tgcccgcgcg
     3961 agtgctgggc aagtgcggcg gttacacctc cgacatcgcc gacgccatcg tctggacgtc
     4021 cggtggcacg gtcacgggtg tgccggcgaa tgccaacccc gccaaggtgc tcagcatctc
     4081 gctgggcggg ggcggcgctt gcgattccac cacgcaaagc gccatcaact cggcgcgttc
     4141 acgcggcgcg aatgtgatcg tggcggcggg caacagcaac gccaactcag ccaacttctc
     4201 cccggcgaac tgctcgggcg tggtgacggt ggcggccgtg ggccgcaatg gtggcaaggc
     4261 gtcgtattcg aacttcggcg cgaccgtcga agtggcggcg cctggcggca gcggcggggc
     4321 caacagcgtg ctgtcgacgc
//
LOCUS       DATDEU010000559         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_840237, whole genome shotgun sequence.
ACCESSION   DATDEU010000559 DATDEU010000000
VERSION     DATDEU010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840237"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VIV63_13335"
     CDS             complement(<1..210)
                     /locus_tag="VIV63_13335"
                     /inference="COORDINATES: protein motif:HMM:NF026171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125628.1"
                     /translation="MKFISTRGQSPSLTFGAALAQGLAPDGGLYVPETWPGIPLSAFD
                     GANTLPEVAEVMLRPFVEGDPVASEI"
     gene            complement(213..1157)
                     /locus_tag="VIV63_13340"
     CDS             complement(213..1157)
                     /locus_tag="VIV63_13340"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY6125629.1"
                     /translation="MSGPGRDQATAFAPASAANVAIGFDILGFSVDVLGDKVTVKKTS
                     RPGVVISGSAGVVKEIPRDPERNTAGRALLAMQDALKPGFGFDAFITKGIPLGSGLGG
                     SAASAVAAVVAANALLDKPLTKIELLQFAMQGEKVSSGSLHVDNISPSLFGGLVLTVG
                     IDNPRVKQIPVPEGIRAVLVHPHLFLSTKQARGILKREVTMSDFIWQTANLAGFISGC
                     YTNDLDMIRASFEDVVIEKQRSQLIPGFDTVRKGALAAGALGCTISGAGPTMFAWALE
                     NDAEKVRQAMVSAFTTANMKVDDWVVPIKTAGAHILPG"
     gene            complement(1154..>2759)
                     /locus_tag="VIV63_13345"
     CDS             complement(1154..>2759)
                     /locus_tag="VIV63_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY6125630.1"
                     /translation="APQKQGSLICAKPDSKLPVKGITSIENIAMVNVEGAGMIGVPGT
                     AHRLFGALREEGISVILISQGSSEHSICCAIPGVEAERAARVVAAAFDREIKEGQIQS
                     IAVDKDLAILAVVGDGMAGLPGVSGQVFNALGSAAVNVHAIAQGASERNISVVVDGKD
                     ATRALRAVHAGFYLSPHTISIGLIGPGSVGRALLEQIAAESGRLRDEFKLDLRLRGIM
                     ASKKMLLAEQGIAVEGWKGALEASPTAADIGKFVDHLHVDHLPHTVIIDCTADDSVAK
                     HYAEWLKSGIHVVTPNKKANSGPLAYYDSLKEARRLGGSSYLYEATVGAGLPVIQTLR
                     DLRETGDKITSVEGIFSGTLAYLFNVYDGKTPFSQIVKDAKAKGYTEPDPRDDLSGTD
                     FVRKVIILGREMGLRLEMKDVQVESLIPAGLEKGTIDDFLNGLSKYDGEMKKRFDAAA
                     ARGKVLRYVGRLTADGKATVGVSELDRSHAFANIALTDNVVRYATARYNSNPLIVQGP
                     GAGPAVTAAGVFADLLRVCSYLGARL"
BASE COUNT          460 a          890 c          918 g          491 t
ORIGIN      
        1 gatttcggag gcgaccgggt cgccttcgac gaacggacga agcatgactt cggccacttc
       61 cggaagcgtg ttcgcgccat cgaacgcgga caacgggatg cccggccagg tctccgggac
      121 gtagagcccg ccgtcgggag ccaggccctg cgcaagtgcg gcgccgaagg tcaaggacgg
      181 tgactggcct cgtgtgctga tgaatttcat gatcagcccg ggaggatgtg tgcgcccgcg
      241 gtcttgatgg gcacgaccca gtcatcgact ttcatgttcg ccgtcgtgaa ggccgagacc
      301 atcgcctggc gcactttctc ggcgtcgttt tcgagcgccc acgcgaacat ggtggggccg
      361 gcgccggaga tcgtgcagcc gagcgcaccc gcggcgagcg cccctttgcg cacggtgtcg
      421 aaaccgggga tgagctgcga gcgctgcttc tcgatgacca cgtcttcgaa cgacgcgcgg
      481 atcatgtcga ggtcgttggt gtaacaaccg gagatgaagc ccgcgagatt cgccgtctgc
      541 cagatgaagt ccgacatcgt gacttcgcgt ttcaggattc cgcgcgcctg cttcgtcgag
      601 aggaacaggt gcggatgcac gagcacggcg cgaatgcctt cgggcacggg gatctgtttc
      661 acgcgcgggt tgtcgatgcc cacggtgagc acgaggccgc caaacaagct cggagaaatg
      721 ttgtcgacgt gcagcgagcc gctcgacact ttctcgccct gcatggcgaa ctgcagcagt
      781 tcgatcttgg tgagcggctt gtcgagcagt gcattcgccg cgaccaccgc cgcgacggcc
      841 gacgccgccg agccaccgag gccgctgccg agcggaatgc ccttggtgat gaacgcatcg
      901 aaaccgaagc cgggcttcag tgcgtcctgc atcgcgagca atgcacggcc cgcggtatta
      961 cgctccggat cgcgcgggat ttccttcacc acgccggccg agccactgat gacgacgccg
     1021 ggccgtgaag tcttcttcac ggtgaccttg tcgccgagca cgtccaccga gaagccgagg
     1081 atgtcgaagc cgatcgccac gttcgccgcc gaggccggcg cgaatgccgt cgcctggtcg
     1141 cgtccggggc cgctcacagt cgcgcaccga ggtacgagca cacgcgcagc agatccgcga
     1201 agacacccgc cgccgtgacg gcgggaccgg cgcccgggcc ctgcacgatg agcgggttgg
     1261 agttgtaccg cgcggtggcg taacgcacga cgttgtcggt gagcgcgatg ttcgcgaacg
     1321 catgcgaccg gtcgagctcg gaaacgccga ccgtcgcctt gccgtccgcg gtgaggcggc
     1381 cgacgtagcg caggactttg cctcgggcgg cggcggcatc gaagcgcttc ttcatctcgc
     1441 cgtcgtactt cgacagacca ttgaggaagt cgtcgatggt gcccttctcg aggcccgccg
     1501 ggatcaggct ttccacctgc acgtctttca tctcgaggcg caggcccatc tcgcggccga
     1561 ggatgatcac cttgcgcacg aagtcggtgc ccgacagatc gtcacgcgga tcgggttccg
     1621 tgtacccctt cgccttggcg tccttgacga tctgcgagaa cggcgtcttg ccgtcgtaca
     1681 cgttgaatag ataggccagc gtgccggaga agatgccttc gacgctcgtg atcttgtcgc
     1741 ccgtctcgcg caggtcgcgc agcgtctgga tgaccggcag gcccgcgccc acggtggctt
     1801 cataaagata agaagagccg cccaggcggc gagcttcttt cagcgaatcg tagtacgcca
     1861 gcggtccgct gttggccttc ttgttcggcg tcacgacgtg gatgcccgac ttgagccatt
     1921 cggcgtaatg cttcgcgacg ctgtcgtcgg cggtgcagtc gatgatgacc gtgtgcggca
     1981 ggtgatcgac gtgcaggtgg tcgacgaatt tgccgatgtc ggcggcggtc gggctcgctt
     2041 ccagcgcgcc tttccagccc tccacggcaa tgccctgctc cgccagcagc attttcttgc
     2101 tggccatgat gccgcgcagg cgcaggtcga gcttgaactc gtcgcgcagt cgcccgctct
     2161 cggcggcgat ctgctcgagc agcgcgcggc ccacggaacc cggaccgatg agcccgatgg
     2221 aaatggtatg cggcgacaga tagaagccgg catgcacggc gcgtaacgcg cgagtggcat
     2281 cttttccgtc gacgaccacc gagatgttgc gctccgaggc gccctgcgcg atggcatgaa
     2341 cgtttacggc cgccgaaccc agtgcattga acacctggcc cgacacaccg ggaagcccgg
     2401 ccatgccgtc gccgaccacg gcgaggatgg cgagatcctt gtcgacggcg atgctctgga
     2461 tctggccttc cttgatctcg cgatcgaacg ccgcggcgac cacgcgcgcg gcgcgctcgg
     2521 cttcgacgcc gggaatggcg cagcagatgg agtgttccga gctgccctgg gaaatgagga
     2581 tgacggagat gccttcttca cgcagcgcgc cgaacagccg atgcgccgtg ccgggcacgc
     2641 cgatcatgcc ggcgccctcc acgttcacca tcgcgatgtt ctcgatgctg gtgatgccct
     2701 tgacgggtag tttggagtcc ggcttcgcgc agatcagcga gccctgcttc tgcggcgcg
//
LOCUS       DATDEU010000560         2550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_400535, whole genome shotgun sequence.
ACCESSION   DATDEU010000560 DATDEU010000000
VERSION     DATDEU010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400535"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VIV63_13350"
     CDS             complement(<1..200)
                     /locus_tag="VIV63_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125631.1"
                     /translation="MTSRTRARCAATAGLLGCLLAASAAQADGLDPVWGAFGETKPIL
                     DTRLRSETVHQDGIPETGHAFTL"
     gene            complement(238..1911)
                     /locus_tag="VIV63_13355"
     CDS             complement(238..1911)
                     /locus_tag="VIV63_13355"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6125632.1"
                     /translation="MSHLELRNVSKNYGDTEVLSNINLSIEEGEFVALIGFSGSGKTT
                     LISILAGLIAPDSGEVRLRGTPAGAAGPDRGVVFQSYSLMPWLTVFENVALAVDSVFS
                     GEDSSARRARTEKYVRMVGLGHAMDRHPAELSGGMRQRVAVARALAMNPEFLLLDEPL
                     SALDALTRAKLQDEIESIWREEKKTVVLVTNDVDEALLLADRIIPLKPGPRATLGPEF
                     KVNLARPRNRKDVNHDPQYRQLRATITQYLLDVEAEKPVPAEGALKLPEVIPITSADF
                     VPKAVRAANKPKPLSNPDKYVEFYNVHKVYPTPRGPLTVVEGFQLDIKRGECVSLIGH
                     SGCGKSTALSMCAGLTDVSDGGITLDGREVTSAGPDRGVVFQAPTLLNWLTARQNVAL
                     GVDRVYPHATSAERRDVVDYYLTRVGLGDALDKRASDMSNGMRQRVGLARAFALSPKL
                     LLLDEPFGMLDALTRWDLQEVLMDILRRTKITAICVTHDVDEAILVADRVIMMTNGPR
                     ARIGKVMQVDLPRPRTRKMLLEHPRYYAYREELLSFLEEYEHGAGNRAA"
     gene            complement(1908..>2550)
                     /locus_tag="VIV63_13360"
     CDS             complement(1908..>2550)
                     /locus_tag="VIV63_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6125633.1"
                     /translation="TYIDQIFTSLKTVFAGFLLATIIAVPLGILCGVSKTFNAMFNPL
                     IQLFKPVSPLAWLPIVTMIVSAVYVSVDPMFDKSFINSAITVTLCSLWPTVINTAVGV
                     GSIDRDLLNVAKVLRLSKTTQLTKLVFPSSLPYIFTGMRLSLGVGWMVLIAAEMLAQN
                     PGLGKFVWDEFQNGSSESLSRIMVAVFTIGIIGFLLDRVMLAMQSAFTYGPTR"
BASE COUNT          472 a          786 c          834 g          458 t
ORIGIN      
        1 agcgtgaacg catgtccggt ctccgggatg ccatcctggt gaaccgtttc cgaacgcagt
       61 cgcgtatcga ggatgggttt ggtctccccg aaggcgcccc agaccggatc gagaccgtcg
      121 gcctgtgcgg cactggccgc gagcaaacat ccgagaagac ctgcagtggc ggcgcaacgc
      181 gcgcgtgtcc gtgacgtcat cgaaaacctc ccaattcgag aaaacaggat tttgttgtca
      241 ggcggcacgg tttcccgcgc cgtgctcgta ctcttccaaa aagctcaata gctcttctct
      301 ataggcgtag tagcgcggat gctcgagcag catcttgcgg gtgcgcggcc gaggcaggtc
      361 gacctgcatc accttgccga tacgcgcgcg cgggccgttg gtcatcatga tcacgcggtc
      421 ggccacgagg atggcttcgt cgacgtcgtg agtgacgcag atggcggtga ttttcgtgcg
      481 ccggaggatg tccatgagga cctcctgcag atcccagcgc gtcaacgcgt ccagcatgcc
      541 gaacggctcg tcgagcagca gtagtttggg cgagagcgcg aaggcgcgcg ccaggccgac
      601 gcgctggcgc atgccgttcg acatgtcgct ggcgcgcttg tcgagggcat cgccaagccc
      661 cacgcgagtg aggtagtagt cgacgacgtc gcggcgttcg gcactggtgg cgtgcggata
      721 gacgcggtcg acgccaagtg caacgttctg ccgcgccgtg agccagttga gcagcgtcgg
      781 cgcctggaac accacgccgc gatcgggacc tgcgctggtg acctcgcgtc catcgagcgt
      841 gatgccgcca tcggaaacgt cggtgagacc ggcgcacatc gacagcgccg tggacttgcc
      901 gcagcccgag tggccgatca gcgaaacgca ttcgccgcgc ttgatgtcga gttggaaacc
      961 ttcgaccacg gtaaggggcc cacgcggagt cgggtacacc ttgtggacgt tgtagaactc
     1021 cacgtacttg tcggggttgg acagcggctt cggcttgttg gcggcgcgca cggctttcgg
     1081 aacgaaatcc gccgaggtga tcggaatcac ttcgggcagc ttgagcgcgc cttccgcggg
     1141 caccggcttt tcggcttcga cgtcgagcag gtattgggtg atggtggcgc gtagctgccg
     1201 gtactgcgga tcgtggttca cgtcctttcg attgcgcggc cgcgcgaggt tcaccttgaa
     1261 ctccgggccg agcgtggcac gcggaccggg cttcagcgga atgatgcggt cggctaacaa
     1321 caacgcctcg tccacgtcgt tggtcacgag cacgacggtc ttcttttctt cgcgccagat
     1381 ggattcgatc tcgtcctgca gcttggcgcg cgtcagcgcg tcgagcgccg acagcggctc
     1441 gtcgagcagc aggaactcgg gattcatggc cagggctcgc gccaccgcca cgcgttgacg
     1501 catgccgcct gacaactcgg cgggatggcg atccatcgcg tggccgagac cgaccatgcg
     1561 tacgtatttt tcagtgcgtg cgcggcgcgc ggacgagtct tcgccgctga acaccgaatc
     1621 taccgccagc gccacgttct cgaacaccgt gagccacggc atgagcgagt agctctggaa
     1681 gacgacgccg cgatcgggcc ccgcggcgcc cgccggcgtg ccgcgcagcc gtacttcgcc
     1741 gctatcgggc gcgatgagcc cggcgaggat ggagatgagc gtcgtcttgc cggagccgga
     1801 gaaaccgatc aacgcgacga actcaccttc ttcgatcgac aggttgatgt tcgacaacac
     1861 ttcggtgtcg ccgtagttct tcgagacgtt gcgcagctcc aggtggctca tcgggtgggc
     1921 ccataggtga acgcgctctg catcgccagc atcacgcgat cgagcaggaa gccgatgatg
     1981 ccgatggtga acacggccac catgatccgg ctcagcgatt cggagctgcc gttctggaat
     2041 tcatcccaga cgaacttccc gaggcccggg ttctgcgcga gcatctccgc ggcgatgagc
     2101 accatccagc ccacaccgag cgacaggcgc atgccggtga agatgtaagg cagcgacgaa
     2161 gggaacacga gcttggtgag ctgcgtcgtc ttgctcagac gcagtacctt cgccacgttg
     2221 agcaggtcgc ggtcgatcga gccgacacca accgcggtat tgatgacggt cggccacagc
     2281 gaacacaacg tgaccgtgat cgccgagttg atgaacgact tgtcgaacat cggatcgaca
     2341 ctgacgtaca ccgcgctcac gatcatcgtc acgatgggca gccacgccag cggcgacacc
     2401 ggcttgaaga gctggatgag cggattgaac atcgcattga aggtcttgct cacgccgcac
     2461 aggattccga gcggcaccgc gatgatcgtc gccagcagga accccgcgaa caccgtcttc
     2521 aggctcgtga agatctggtc gatgtaggta
//
LOCUS       DATDEU010000561         8134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_400614, whole genome shotgun sequence.
ACCESSION   DATDEU010000561 DATDEU010000000
VERSION     DATDEU010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400614"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1358)
                     /locus_tag="VIV63_13365"
     CDS             complement(153..1358)
                     /locus_tag="VIV63_13365"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEY6125634.1"
                     /translation="MLTKTKSRKSRPTRAFIDPFTQPEIKRLVAEFGSPLLIIDCQRV
                     REQYRKLHKALPGVDLHYALKPLPHPAVVQTIVDMGGWLDLATTGETQLVTQLGVDPA
                     RCIHTHPIKRDIDIRNALHLGVKIFVADNPDEVRKFEAYRDQCELLLRVSFRSPGAVC
                     DLSRKFGCDPEDLLDLARLARKLGIPVRGLSFHVGSQASDAGKHVEAIEACGKLIAAA
                     KREKLGLFDTLDIGGGFPIEYAQKVADIGRFCAPIRAALAKLPKKLRIIAEPGRYISG
                     PAAIGVSSVMGRAKREGHWWYYLDDGLYGSYSGQLFDHAKYPVESLKESDEKFPSVLA
                     GPTCDSIDVIAENMMLPALKAGDLVVGRAMGAYTWASASEFNFFPKATVVSVNLQPGD
                     TGSVIPLPF"
     gene            1498..2997
                     /locus_tag="VIV63_13370"
     CDS             1498..2997
                     /locus_tag="VIV63_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6125635.1"
                     /translation="MAPDSSPAGELQTLLASRVAMVVIESREESRVMELVREAALKAQ
                     RGKNWGVFQWTVTEGLLRVDVDLGGAQKTLAQPDQLLKHIRATNMAGIYVLLDFHPYL
                     ENNPMCVRTLKDIAHDYQKCARTLVLISHEVKLPQELEHLAARFSLQMPDKAERQAIV
                     MKMAREWAQNTGGMPRIDVKVVEKLVENLAGLPLHDVERLTRQAIFNDGALTEDDLRP
                     LMAAKYQLLNRGGTLSFEADTARFAEVGGLKNLRRWIQQRKAAFDGSAPGLDAPKGVL
                     LLGVQGCGKSLAARAAAGVLGVPLVRLDFGALYSKWHGESEKNLRESLASAEALSPCV
                     LWLDEIEKALSAGDGDSGTSRRVLGAFLTWLAEQRARVFIVATANDITALPPELVRKG
                     RFDEIFFVDLPTPAARLEIFGIHCKKRAVVIPEAEMKALAARSEGFSGAEIEQAVVSA
                     LYSAHANRQPVDAKLIAGEIDATRPLSVVMGEKIAELRAWAAERTVPAD"
     gene            complement(3015..3503)
                     /locus_tag="VIV63_13375"
     CDS             complement(3015..3503)
                     /locus_tag="VIV63_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125636.1"
                     /translation="MNTQMESMAMVRKLAQGELSVRARLGYVALLLASSAMTVVILSL
                     WLTEPVLPLRAQIAFGVMSLIGISWITLAIWALATRRILLARDRVIAGRMSVAFTALF
                     LVGALAAVFMTFSAAAYGAAATGGVMFVVAMRVLAGAKRRFAELAARRAELEMGSGPI
                     SR"
     gene            complement(3500..4033)
                     /locus_tag="VIV63_13380"
     CDS             complement(3500..4033)
                     /locus_tag="VIV63_13380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6125637.1"
                     /translation="MDPRTDELLAVRCQLGERAAFDALIARWHEPLWRYLRRLADSDD
                     TARDLAQDTWLRVFRGIAKLREPARVRPWLFGVARNVAMDRLRAKYSHGSHVDVDIDD
                     IAAVADDANLEEEFATLESGLATLPIIDRETLTLFYLRELTIEEIAGLVGAPVGTVKS
                     RLFRARQQLRRVMEGKQ"
     gene            complement(4157..5719)
                     /locus_tag="VIV63_13385"
     CDS             complement(4157..5719)
                     /locus_tag="VIV63_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY6125638.1"
                     /translation="MDLTTAFLPVVSFVIVTFITIIFVFTRYKRCPSDKILVVYGKVG
                     KGQSAVCIHGGGAFIWPVFQDYTYMSLTPLTIPIPLTKALSLQNIRVNVPSTFTVGVS
                     TEEAIMNNAAERLLNLAQDDVESMAREIIFGQLRLTVASLTIEQINQDREKFLDAIRR
                     NVAPELNKIGLYLINVNITDITDESGYIESIGKKAASEAINQAKVDVSDQDRKGSVGE
                     AEQVRERTIKVAQHLAESEKGKKQAEADKRIYVQAQETLATVGEAEANRDMQIKVAQN
                     EATSIQGQKKAEAEKRIFVEAQEAEAIAGENRAKALIAQSNAELFVQSAQASQRGQVA
                     QFDAEAEIQRAQAKAELQRLTAQEVVRQQVEKTKITIAAEAEAEKTRREAQGVADATL
                     LKYEAEAKGIRQVLEAKAQGYQALVMSANNDARAAATLLLTEKLEHLVAMQVEAIKAI
                     KIDKVTVWDSGAGANGNGGTTTSNFLAGMVKSLPPLHDVAAMAGLDLPKFLGDANVTA
                     KQDGNGLLPPPK"
     gene            complement(5769..6326)
                     /locus_tag="VIV63_13390"
     CDS             complement(5769..6326)
                     /locus_tag="VIV63_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY6125639.1"
                     /translation="MTEWFASYSGVEQMFLLAGIVGGIILVFRLILTIAGLDHHGDMT
                     SGHGDGDGAFQALTIQGVSSFFTMFGVVGFTLYRNASVGIALSLIGALAAGSLSVWII
                     HKMFTAMLRLQSSGTVSLFAAVGSEGSVYLTVGKDGGRVQINFANRLREFEAVSADGG
                     TIPTGTAIRVQSVAANTLVVAPVDR"
     gene            complement(6390..7214)
                     /locus_tag="VIV63_13395"
     CDS             complement(6390..7214)
                     /locus_tag="VIV63_13395"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY6125640.1"
                     /translation="MKPHRLPVYFISHGGGPWSYMDDHSRAPYAKLEASLAAMPREIG
                     VTPRAILMISAHWEEPEFTLTSNPKPPMIYDYGGFPDYTYHIRYDAPGDPSLAARIKA
                     LLDAAGLPARLDANRGFDHGAFTPLKVMYPEADVPVVQLSLKRGLDPATHLAMGRALS
                     PLREEGVLIVGSGLSYHNLRQFFSPAGFGPSREFDAWLNGALRGGSSADRGKLLTAWE
                     TAPSARAAHPREEHLLPLMVAVGAAGDDMTELTYHEKDFLGGLTVSSYRFSKSPAS"
     gene            7275..>8134
                     /locus_tag="VIV63_13400"
     CDS             7275..>8134
                     /locus_tag="VIV63_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125641.1"
                     /translation="MHSWIRGWIAAFVILACVNSATASAATARLYFDPASGPQTFAAG
                     DIESALRARKYSVTRHDSHELRSPPRKADVLILFERTPPGSKDVKAEGFIVTPASHGG
                     TAAIRVISDDAAGAMYGGLELAEQIRIHGVEGVRDTRRNPHMSMRGTKFNIPLDLRSP
                     SYSDMSDSAQENIATVWDFEFWRAYLDKLARDRYNFVSLWNLHPFPSMVKVPEYPDVA
                     LQDVWRSKIKFDEDYSTRTVGLLTPAMLANKEVVKQLTIAQKIDFWRRVMQYAKDRNI
                     EFYVMTWNVFT"
BASE COUNT         1454 a         2590 c         2627 g         1463 t
ORIGIN      
        1 ctttcgccgt ggagtcggca ttggcgccgc cctgcgccga cgcgatgcgc gccagcgaca
       61 gccccgcgcc gcccgcgagc agggtctgta tccatcggcg tcttgaccag ttttgcgaca
      121 tcaggattgg agtctggccc ctggcgcttg gttcaaaacg ggagcggaat cacagagccg
      181 gtatcgcccg gctggaggtt gacggaaacc accgttgcct tagggaagaa gttgaattcg
      241 gaggcgctgg cccaggtgta ggcgcccatc gcacgcccga ccaccaggtc tccggctttc
      301 aacgccggca gcatcatgtt ttccgcgatc acatcgatgc tgtcgcaggt ggggcccgcg
      361 agcaccgacg ggaatttctc gtcgctttcc ttcagcgact ccaccgggta tttcgcgtgg
      421 tcgaacaact ggccgctgta cgaaccatag aggccgtcgt cgaggtagta ccaccaatga
      481 ccttcacgct tcgcgcggcc catgaccgac gagacgccga tcgccgccgg acctgaaatg
      541 tatcgtccgg gctcggcgat gatgcgcagc ttcttcggaa gtttggcgag cgcggcgcgg
      601 atcggcgcgc agaagcggcc gatgtcggca accttctgtg cgtactcgat gggaaagccg
      661 ccaccgatgt cgagcgtgtc gaacaggccg agcttctcac gcttcgcggc ggcgatcagc
      721 ttgccgcagg cctcgatggc ctcgacgtgt ttgccggcgt cgctcgcctg cgagcccacg
      781 tggaacgaga gcccgcgcac ggggatgccg agcttgcgtg ccagccgcgc gaggtccagc
      841 aggtcttcgg ggtcgcaacc gaactttctc gacagatcgc agaccgcgcc gggcgaacgg
      901 aacgacacgc gcagcagaag ttcgcactgg tcgcgatagg cttcgaactt gcgcacctcg
      961 tccggattgt cggcgacgaa gatcttcacg ccgagatgca gcgcgttgcg gatgtcgatg
     1021 tcgcgcttga tgggatgtgt gtggatgcag cgcgccgggt cgacgccgag ctgggtcacg
     1081 agctgcgttt cgccggtggt cgcgaggtct aaccagccgc ccatgtcgac gatggtctgc
     1141 acgacggcgg gatgcggcag cggcttgagc gcgtagtgca ggtctacgcc gggcaacgcc
     1201 ttgtgcagct tgcggtactg ctcgcggacg cgctggcagt cgatgatgag cagcggcgag
     1261 ccgaactccg ccaccagccg tttgatctcg ggctgggtga acgggtcgat gaaggcacgc
     1321 gtgggccgtg acttgcgtga tttcgtcttc gtgagcaccg ctgtctcctg ccggacagcc
     1381 cggcggaaaa gcggcgcatt gtaggcgggc tggccgtcag tgtcatgacc ggccgatgag
     1441 acgtgaacga ggcccccgaa cagggcgttt tgctaaagtg ccgcgcacat gaaaaatatg
     1501 gctcctgaca gctcccccgc cggagaactg caaaccctgt tggcttcgcg cgtggcgatg
     1561 gtggtcatcg aaagccggga agaatcccgg gtcatggagc tggtacgcga agcggccctg
     1621 aaggcccagc gcggcaagaa ctggggcgtc ttccagtgga ccgtgaccga aggcttgctg
     1681 cgcgtcgacg tcgatctggg cggcgcgcag aagaccctgg cgcagcccga ccagctgctc
     1741 aagcacatcc gcgccaccaa catggccggc atctacgtgc tgctcgactt ccacccctat
     1801 ctcgaaaaca acccgatgtg cgtgcgcacg ctcaaggaca tcgcgcacga ctaccagaaa
     1861 tgcgcgcgca ctctcgtgct gatcagccac gaagtgaagc tgccgcagga gctcgagcat
     1921 ctcgcggcgc gtttctcgct gcagatgccg gacaaggcgg aacggcaggc catcgtcatg
     1981 aagatggcgc gcgaatgggc gcagaacacc ggcggcatgc cgcgcatcga cgtgaaggtg
     2041 gtggagaagc tcgtcgagaa tctcgccgga ctgccgctgc atgacgtaga gcggttgacg
     2101 cgccaggcca tattcaatga cggcgcgctc accgaagacg acctgcgccc gctgatggcg
     2161 gcgaaatatc aattactgaa tcgcggcggc accctgtcgt tcgaggcgga taccgcgcgc
     2221 ttcgccgagg tcggtggact gaagaacctc aggcgctgga tccagcagcg caaggcggcg
     2281 ttcgacggca gcgcgccggg tctcgacgcg cccaagggcg tgctgttgtt aggtgtgcag
     2341 ggctgcggca agagcctggc ggcgcgtgcc gccgccggcg tgctgggcgt gccgctggta
     2401 cgactcgatt tcggcgcgct ctattcgaaa tggcatggcg aatcggaaaa gaatctgcgc
     2461 gagtcgctgg cgtccgccga ggcgctctcg ccgtgcgtgt tgtggctcga cgaaatcgag
     2521 aaggcgctgt ccgccggtga cggagacagc ggcacctcac gccgcgtgct gggcgcgttc
     2581 ctcacctggc tcgccgagca gcgtgcgcgt gtattcatcg tggcgaccgc caacgacatc
     2641 acggcactgc cgcccgaact cgtgcgcaag ggccgcttcg atgaaatctt cttcgtcgac
     2701 ctgcccacgc cggccgcgcg gctcgagatc ttcggcattc attgcaagaa acgcgcggtg
     2761 gtgatcccgg aagccgagat gaaggcgctg gcagcgcgaa gcgaagggtt ctccggcgcg
     2821 gagatcgagc aggccgtggt gtcggcgctc tacagcgcgc atgcgaaccg ccagccggtg
     2881 gatgcgaagc tcattgccgg cgaaatcgac gcgacccggc cgttgtcagt ggtgatgggc
     2941 gagaagattg cggagctgcg cgcgtgggca gcggagcgca ccgtccccgc cgactgaaat
     3001 ggggtcagac cccattaccg ggaaatgggg cctgacccca tttcgagttc ggcgcgccgc
     3061 gcggccagct ccgcgaaacg ccgcttcgca cccgcgagta cacgcatcgc gacgacgaac
     3121 atgacgccgc cagtcgccgc ggctccataa gccgcggcac tgaaggtcat gaataccgcc
     3181 gcgagggcgc ccaccaggaa cagcgcggtg aaggccacgc tcatgcgccc ggcaatcacc
     3241 cgatcgcgcg ccagcaggat gcgccgcgtg gccagcgccc agatcgcaag cgtgatccac
     3301 gagattccaa tgaggctcat cacgccgaaa gcgatctgcg cgcgcaacgg cagaacgggt
     3361 tccgtgagcc acagcgacag gatgaccacc gtcatcgccg aagacgccag cagcagcgcg
     3421 acatatccca accgcgccct gaccgataac tcgccctgcg cgagcttgcg caccatcgcc
     3481 atcgattcca tttgcgtgtt cattgtttac cttccatcac acgacgtaac tgctggcgag
     3541 cacgaaacag tcgtgatttg accgtgccca cgggcgcgcc caccaggcct gcgatctcct
     3601 cgatcgtgag ctcacgcagg tagaacagcg tgagtgtctc gcgatcgatg atcggcagag
     3661 ttgcgagtcc cgattccaga gtcgcgaatt cctcttcgag gttcgcgtca tcggcgactg
     3721 ccgcgatgtc gtcgatgtcg acgtccacgt gactgccgtg cgaatacttc gcacgcagcc
     3781 ggtccatggc gacgttccgc gccacaccga acaaccaggg ccgcactctt gcgggttcgc
     3841 gcagcttcgc gatgccgcga aacacgcgca gccaggtgtc ctgggcgaga tcgcgtgcgg
     3901 tgtcgtcgct gtccgcgagc cgccgcaggt agcgccacag cggttcgtgc cagcgcgcga
     3961 tgagcgcgtc gaacgcggcg cgctcgccga gctggcagcg caccgccagc agctcgtcgg
     4021 tgcgcgggtc catgtgaagg tttttggggg tcacggacgt gaagtcgcgc ggggtggcaa
     4081 aaggttcacg caaagagaaa gggccgcacc ccatcgaggt gcggcccctt ttgctaggaa
     4141 atggggagtg tccccattat ttcggcggcg gcagcagccc gttgccgtcc tgcttcgccg
     4201 tcacgttggc gtcgcccagg aacttgggca gatcgagccc cgccatcgcg gccacgtcgt
     4261 gcagcggcgg caggctcttc accatgccag ccaggaagtt ggacgtggtg gttccaccat
     4321 tgccattggc gcccgcgccg ctatcccaca ccgtgacctt gtcgatcttg atggccttga
     4381 tggcttcgac ctgcatggcc accaggtgct ccagcttctc ggtgagcagc agggtcgcag
     4441 ccgcacgtgc atcgttgtta gcgctcatga cgagcgcctg gtagccctgc gccttcgcct
     4501 cgagcacctg ccggataccc ttggcttcgg cctcgtactt gagcagcgtg gcatccgcga
     4561 cgccctgcgc ttcgcgacgg gtcttctcgg cttcggcttc cgcagcgatc gtgatcttgg
     4621 tcttctcgac ctgctggcgc acgacctcct gggccgtgag gcgctgaagt tcggccttcg
     4681 cctgtgcgcg ctggatctcg gcctcggcat cgaactgcgc aacctggcca cgctgcgacg
     4741 cctgcgccga ctgcacgaac agctcggcgt tggactgcgc gatcagcgcc ttcgcgcggt
     4801 tctcgccggc gatggcctcg gcttcctgcg cttcgacgaa tatgcgtttt tcggcttcgg
     4861 ccttcttctg accctgaatc gacgtggctt cgttctgcgc caccttgatc tgcatgtcgc
     4921 gattggcctc ggcctcgccg accgtcgcca gcgtctcctg cgcctgcaca tagatgcgct
     4981 tgtcggcttc ggcctgcttc ttgcccttct ccgattccgc gaggtgctgc gcgaccttga
     5041 tggtgcgctc gcgcacctgc tcggcttcgc cgaccgaacc cttgcggtcc tggtcggaga
     5101 cgtcgacctt ggcctggttg atggcttccg acgccgcctt cttgccgatg ctctcgatgt
     5161 atcccgactc gtcggtgatg tcggtgatgt tcacgttgat caggtacaga ccgatcttgt
     5221 tgagctcggg cgccacgttg cggcggatcg cgtcgaggaa tttttcgcga tcctggttga
     5281 tctgctcgat cgtgagcgag gccaccgtga ggcgcagctg accgaagatg atttcgcgcg
     5341 ccatgctctc gacgtcgtcc tgcgcgagat tcaacaggcg ctcggccgcg ttgttcatga
     5401 ttgcttcttc ggtgctgaca cccaccgtga acgtgctcgg cacgttgacg cggatgttct
     5461 gcagcgacaa cgccttggtc agcgggatcg ggatggtgag cggcgtcagg ctcatgtacg
     5521 tgtagtcctg gaagaccggc cagatgaacg cgccgccgcc atggatacag accgctgact
     5581 ggcccttgcc caccttgccg tacaccacga ggatcttgtc ggatgggcac cgcttgtagc
     5641 gtgtgaacac gaagatgatg gtgatgaacg tcacgatgac gaacgagacc accggcagga
     5701 atgcggttgt gagatccatg cgagaccctc cgcgcgcagg ctgcgcgcaa actaactaat
     5761 agacccgatc agcgatcaac gggagcgacg accagcgtat tggccgcaac gctttgcaca
     5821 cgaatggccg ttcccgtggg aatggtccct ccgtcggccg aaaccgcctc gaactcgcgc
     5881 aggcgattgg caaagttgat ctgcacgcgg ccgccgtcct tgcccaccgt gagatacacc
     5941 gagccttcgc tgccgaccgc ggcgaacagg ctgacggtgc cgctcgactg caggcgcagc
     6001 atggccgtga acatcttgtg gatgatccag acagacaggc ttccggcggc aagagcgccg
     6061 atgagggaca gggcgatgcc gaccgatgcg ttgcggtaga gcgtgaatcc cacgacaccg
     6121 aacatcgtga agaacgagga gacgccctgg atggtgaggg cctggaaggc accgtcgccg
     6181 tcaccgtgac ccgaggtcat gtcgccgtgg tgatcgagtc cggcgatggt gagaatgagt
     6241 ctgaagacca gaatgattcc gccgacgata ccggccaata aaaacatctg ttcgacgccg
     6301 ctgtacgaag caaaccactc agtcatggcc gcaactcccg ccggatgttt tgattggttg
     6361 tcccggcaat ggggactata gcgaggattt caagacgccg gggattttga aaagcgataa
     6421 ctactgacgg tcagtccgcc gagaaaatcc ttctcgtgat atgtcagttc cgtcatgtcg
     6481 tcgccggcgg cgcccaccgc caccatcagc ggcagcaggt gctcctcgcg aggatgcgcc
     6541 gcgcgcgcgg agggcgccgt ttcccaggcg gtcagtagtt tgccgcggtc ggccgaactc
     6601 ccgcctcgca gcgcgccatt cagccaggcg tcgaattcgc gcgaagggcc aaatccggca
     6661 gggctgaaga actggcgcag gttgtgatag ctcaacccgc tgccgacgat cagcacccct
     6721 tcctcgcgta gcggggagag agcgcgaccc atcgcgagat gcgttgcggg atcgagcccc
     6781 cgcttgagcg agagctgcac cacgggcacg tcggcctccg ggtacatgac cttgagtgga
     6841 gtgaacgcgc catgatcgaa tccgcggttc gcgtcgaggc gagcgggcag ccccgccgca
     6901 tcgagcaacg ccttgattcg cgccgccagc gacgggtctc cgggcgcgtc atagcgaatg
     6961 tggtaggtgt agtcgggaaa accgccgtag tcgtagatca tcggcggctt cgggttcgac
     7021 gtcagcgtga actccggttc ctcccagtgc gcggagatca tcaggatggc gcgcggcgtg
     7081 acgccgatct cccgcggcat ggcggccagt gatgcctcga gcttcgcgta cggcgcgcgc
     7141 gagtgatcgt ccatgtacga ccacgggccg ccgccgtgcg agatgaaata caccggaagt
     7201 cgatgaggct tcatttgact gccagtgtac gcttccgcct cgctttcacg gtcggtgaac
     7261 aacaagggga attcgtgcac tcctggattc gcggatggat tgcggccttc gtcattctgg
     7321 cctgcgtgaa tagtgcaact gcgtcggccg ccaccgcacg cctctacttc gatccggctt
     7381 cggggccgca gaccttcgcc gccggcgaca tcgagtccgc gctgcgcgcg cggaagtatt
     7441 cggtgacgag gcacgactcg cacgagctcc gctcacctcc ccgcaaggct gatgtcctca
     7501 tcctgttcga acgcactccg ccgggttcga aggatgtgaa ggcggaagga ttcatcgtga
     7561 ctccggcgtc ccatggcggg acggccgcga tccgcgtcat cagcgacgac gcggccggcg
     7621 ccatgtatgg cgggctcgaa ctggcggaac agattcgcat ccacggcgtt gaaggcgtca
     7681 gggacacccg gcgcaacccg cacatgtcca tgcgcggcac caagttcaat attcctctcg
     7741 acctgcgcag ccccagctac tccgacatga gcgactccgc gcaggagaac atcgccaccg
     7801 tctgggactt cgaattctgg cgtgcctatc tcgataagct ggcgcgcgac cgctacaact
     7861 tcgtgtcgct gtggaacctg caccctttcc catcgatggt gaaggtgccc gaataccccg
     7921 acgtggcgct gcaggatgtc tggcgatcga agatcaagtt cgacgaggac tattcgacgc
     7981 gcaccgtagg gctgctgacg cccgcgatgc tggccaacaa ggaagtcgtc aaacagctca
     8041 ccatcgcgca gaagatcgac ttctggcgac gcgtcatgca atacgcgaag gaccggaaca
     8101 tcgagttcta cgtgatgacg tggaacgtgt tcac
//
LOCUS       DATDEU010000562         3390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1133385, whole genome shotgun sequence.
ACCESSION   DATDEU010000562 DATDEU010000000
VERSION     DATDEU010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133385"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VIV63_13405"
     CDS             complement(<1..378)
                     /locus_tag="VIV63_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125642.1"
                     /translation="MKLARVFAVLTLPFAGLAPAQSADSPAVPVVVTTVRSENFGASL
                     TATGTVVSRNDARISGEVGGTLSWIAEPGIAVKRGDVIARIAPERLALTLRDNEAVVK
                     RLEAQLSLLATQRTRLQTLGNVVS"
     gene            complement(375..>3390)
                     /locus_tag="VIV63_13410"
     CDS             complement(375..>3390)
                     /locus_tag="VIV63_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6125643.1"
                     /translation="FALIKLPIQLFPNIEEPVISIFTSGRAAAPTEIESEIIEPQERA
                     LAGLRGMQSLNAFANAGSAFVNLRFAVGTDMQATMLEVISRMNQLPPLPRDAQAPQIS
                     LGEDGGNGPNNTLSWFFVQLLPGTAGPIDNYRRQIEELFRNRIESIPGVANVRINFGS
                     EEELQIVFDPARAAELGIQIPRIAQVAGSADDVSGGFVDIGRRQYTMRFAGRYSMEQF
                     TDLVIEWRDGRPVRLGDIATVQVRRGDRSDLALQNGNPAIGIQIMKENDANVLDTLTA
                     VKAEIASLRDNELKKLGLSIAQSFDPSVFIDQAIDMVTGNLFAGILLAVAVLWFFVRD
                     RRATLLVGLSIPICLTVTLVMLYAFGRTLNVISLAGLAFGVGQALDTAIVMLEAIAQQ
                     RERGLARIEATLAGARQVWPALLASTVTAVIVFLPIVFMRDAVSQLFADLALTISIAV
                     IASLIVAVTALPLAASRWLNAEDVHKGSNDRYAAVANWIVRWTDSPKRRYLTVGVLFV
                     APVVLTWALLPPLDYLPPVKRDAIDGFIQLPPGTNIDTVEQEFVKPVAERLAPYMAGE
                     KQPALKNYYILAGAFGTTIGVRPLDPDNIGELGRLINEEVLVGFPDTQSFAAQGNLFG
                     GFGDGRNIDFQLQATDFPALLAAARFAEKLIQEKMPGAQVQAFQGLEMAEPELRLNPD
                     DRRLNEIGWTRADIGTVVRALGDGMFVGEYFDGERRLDMILRASKWDSPEALAETPIA
                     TPFGSVMPLGELVRVESTVGAGGLRRVDGHRTIGLNVSPPKELSLGQAIEVLKRDVEP
                     QVRAELPADGSVHYEGNAGSLREALSNMAENLAIALIALFLLLAALFRSVKDSMYVIL
                     TIPLASFGGVLALVLLRLFTPQTLDLLTMVGFVVLMGLVVNNAILLVDQARSEIREGA
                     TVRAAVESSLATRTRPIMLTTLTTLFGMLPLVLIPGPGSVLYRGLGTVLVGGMAVNSV
                     FTLVLLPTLLRLIEKPATALSDQPGRQPIGAI"
BASE COUNT          599 a         1069 c         1154 g          568 t
ORIGIN      
        1 cgacacgaca ttgcccagtg tctgcagccg cgtgcgctgc gtggctaaca aggacagttg
       61 cgcttcgagc cgtttgacca ccgcctcgtt gtcgcgcagc gtgagtgcga gccgctcggg
      121 cgcaatgcgt gcgatgacgt cgccgcgctt caccgcgatg ccgggctcgg cgatccacga
      181 gagcgtgccg cccacttcgc cggagatgcg cgcgtcattg cggctgacca ccgtgccggt
      241 ggcggtgagg ctggcgccga aattctccga ccgcaccgtg gtgacgacca cgggcacggc
      301 aggtgaatcg gcgctctgcg ccggcgcgag gccggcgaat ggaagtgtca ggacggcgaa
      361 cacgcgcgcg agcttcatat ggctccaatg ggttgtctgc cgggttggtc cgacaaggcg
      421 gtggcaggct tttcgatgag gcggagcaaa gtagggagaa gtactagcgt gaacaccgag
      481 ttcacggcca tgccgcccac cagcaccgtg ccgaggccgc gatacaggac gcttccgggg
      541 ccgggaatca gcaccagcgg caacatgccg aacagcgtgg tcagtgtggt cagcatgatg
      601 ggccgcgtgc gcgtggccag agacgactcg accgccgcgc gtaccgtggc gccttcacgg
      661 atctcgctgc gcgcctggtc gaccagcagg atcgcgttgt tgacgacgag gcccatgagc
      721 acgacaaagc cgaccatggt cagcaggtcg agcgtctgtg gcgtgaacag ccgcaacagc
      781 accagcgcca gcacgccgcc gaagctggcg aggggaatcg tgaggatcac gtacatgctg
      841 tctttgaccg accggaacaa cgcagccagc agcaggaaca gcgcgatcaa cgcgatcgcc
      901 aggttctccg ccatgttcga caacgcttcg cgcagtgaac cggcattacc ttcgtagtgc
      961 accgagccat cggcgggcag ctcagcccgc acctgcggct cgacatcgcg cttcaggacc
     1021 tcgatggcct gaccgagtga gagctctttg ggcgggctca cgttgagccc aatggtccgg
     1081 tggccatcga cgcgccgcag gccgcctgcg cccaccgtgg actcgacgcg gaccagttcg
     1141 ccgagcggca tcacgctgcc gaatggcgtc gcgatagggg tttccgccaa cgcctcgggc
     1201 gagtcccatt tgctggcccg caggatcatg tccaggcgcc tttcgccgtc gaaatattcg
     1261 ccgacgaaca ttccgtcgcc gagcgcccgc accacggtgc cgatgtcagc gcgcgtccag
     1321 ccgatttcgt tgagccgccg gtcgtcgggg ttcagtcgca gctccggctc cgccatttcc
     1381 aggccctgga acgcctgcac ctgcgcgccg ggcatctttt cctggatgag tttctcggcg
     1441 aatcgcgcgg ccgccagcaa tgcggggaaa tccgtggcct ggagctggaa atcgatgttg
     1501 cggccatcac cgaagccgcc gaacaggttg ccttgcgcgg cgaaggactg ggtgtcggga
     1561 aacccaacca ggacctcttc gttgatgagc ctgcccagct cgccgatgtt gtcgggatcc
     1621 agcgggcgca cgccgatcgt cgtgccgaac gccccggcga ggatgtaata gttcttgagc
     1681 gcgggctgct tttcgccggc catgtaaggc gcgaggcgtt cggccaccgg cttcacgaat
     1741 tcctgctcga ccgtgtcgat gttcgtgccc ggcggaagct ggatgaatcc gtcgatggca
     1801 tcgcgcttca cgggaggcag gtagtcgagc ggtggcagca aggcccaggt gaggaccacc
     1861 ggagccacga acaacacgcc gaccgtgagg taccggcgct tcggcgagtc ggtccagcgc
     1921 acgatccagt tcgcgacggc cgcgtaccgg tcgttagagc ccttgtgcac gtcctcggca
     1981 ttcagccagc gcgaagccgc cagcggcaat gccgtcaccg cgacgatgag cgaggcgatg
     2041 accgcgatgg aaatcgtgag ggccagatcg gcgaacaatt gagacaccgc atcgcgcatg
     2101 aacacgatgg gcaggaagac gatgaccgcc gtcaccgtgc tcgcgagcag cgccggccag
     2161 acctgcctgg cgccggcaag tgtggcttcg atgcgggcga ggccgcgctc acgttgctgc
     2221 gcgatggcct cgagcatgac gatggcggtg tcgagcgcct gccccacgcc gaaggcgaga
     2281 cctgccagcg agatgacgtt gagcgtgcgg ccaaacgcat agagcatcac cagcgtgacc
     2341 gtgaggcaga tcgggatcga cagcccgacc agcaacgtgg cgcgccggtc gcgcacgaag
     2401 aaccagagca ccgcgacggc gagaaggatt ccggcgaaca ggttgccggt gaccatgtcg
     2461 atcgcctggt cgatgaacac cgaaggatcg aacgactgcg cgatcgacag gcctaacttc
     2521 ttcaattcgt tgtcgcgcag cgaggcgatc tccgccttga ccgcggtcag cgtgtcgaga
     2581 acgttggcgt cgttctcttt catgatctgg atgccgatgg ccgggttgcc gttttgcagc
     2641 gcgagatcgc tgcggtcgcc gcgccgaacc tgcacggtgg cgatgtcgcc gagccgcact
     2701 ggccggccgt cgcgccactc gatcacgagg tcggtgaact gctccatcga ataacggccc
     2761 gcgaatcgca tcgtgtactg acggcggccg atatccacga agccgcccga gacgtcatcg
     2821 gcgctgccgg cgacctgcgc gatgcggggt atctggatgc cgagctcggc ggcgcgcgcc
     2881 ggatcgaaca cgatctgcag ttcttcctcg gagccgaagt tgatgcgtac gttggcgaca
     2941 ccggggatgg attcgatgcg attgcggaac agctcttcga tctggcggcg gtagttgtcg
     3001 atgggtcccg cggtgccggg cagaagctgc acgaagaacc aggagagcgt gttgttgggg
     3061 ccgttgccgc cgtcttcgcc gagcgagatc tgcggcgcct gcgcgtcgcg cggcaacggc
     3121 ggcagctggt tcatgcggct gatgacttcg agcatcgtcg cctgcatgtc ggtgccgact
     3181 gcgaatcgca ggttgacgaa tgcgctgccg gcgttggcga aggcgttcag cgattgcatg
     3241 ccgcgcagac ccgccagggc ccgctcctgc ggttcgatga tctcggattc gatctcggtg
     3301 ggtgccgccg cgcgcccgct cgtgaagatc gagatgaccg gctcttcgat gttcgggaag
     3361 agctggatcg gtagtttgat cagtgcgaat
//
LOCUS       DATDEU010000563         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_840984, whole genome shotgun sequence.
ACCESSION   DATDEU010000563 DATDEU010000000
VERSION     DATDEU010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840984"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VIV63_13415"
     CDS             complement(<1..707)
                     /locus_tag="VIV63_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125644.1"
                     /translation="MKPRRGPTDTVTTAPRPPPPPPPPDRSRDLVAPTRTPPPRDREP
                     AAEERSAEPNPESTNSAPPAALSDPGVPEAPAAKPANALKAELLVASQDVAESDAQRA
                     WMQSQGAQLLRRRTLSHLGWVLSVYRLSPGASASAVKAALLQQWPDSIPENNERYQAL
                     GESAAAPAEYAGPLIHWPVAGCGRRPRIAMLDGPVNTAVAALAGARVQSAVFTPAGQP
                     NYHHGTSVAAVLVGGAMP"
     gene            complement(794..1381)
                     /locus_tag="VIV63_13420"
     CDS             complement(794..1381)
                     /locus_tag="VIV63_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125645.1"
                     /translation="MTAASFDVSALIPWYLNGTLSDAERAQVEKFLRESPDAEPQLQM
                     WRAVQLQTRTQPLADPGTELGWRGFRRELGRVKARDSMRLWRITAAASVLLIAGLQTA
                     ILMRQDDADRYAPLSGTTAVPADSWRVQVRFVETATVADINALLLRIDARVIRGPSAL
                     GIYEIILPRRPGDGDAAVRATLQAEPLLLQAVVMP"
     gene            complement(1378..1971)
                     /locus_tag="VIV63_13425"
     CDS             complement(1378..1971)
                     /locus_tag="VIV63_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6125646.1"
                     /translation="MNATLSPSLADAEAQSRRWIERIALGEEAALEQFYRHYHGQVFQ
                     FALRLVNNAADAAELANETMMEVWRAAARFAGDSRVRTWLFGIVNHRAIDLLRRRRRR
                     HEHAEELDDVADDAPACSISDVIGGAQDARHVRSCVEQLPERQKAVVHLAFFEELSYP
                     EVAQVLDVPVGTIKTRVMHAKQRLLHCLAGLFARGST"
     gene            complement(1980..>2410)
                     /locus_tag="VIV63_13430"
     CDS             complement(1980..>2410)
                     /locus_tag="VIV63_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125647.1"
                     /translation="MSGRYTLRFCIEDGAVPLLSCADSASTVQTNYQVEPGEAAAVIR
                     GVWPDNQDLGASEPLRWPAVKGTTTYQLQIFEPATVQGADPEFVTGLLLPGNSASAPL
                     GELALRKLVSGHRYLWRVTAHDLDGRLIARSDLAPFVLRQ"
BASE COUNT          386 a          820 c          817 g          387 t
ORIGIN      
        1 ggcatcgcgc cacccacgag tacggccgcg accgaggttc catgatggta gttaggctga
       61 ccggccggcg tgaatacggc cgactgaacc cgggcgccgg cgagcgccgc caccgccgtg
      121 ttcaccggac catcgagcat cgcgatgcgc gggcggcgcc cgcagccagc cactggccag
      181 tggatcaatg gacctgcata ttccgccggt gccgcggcgg attcacccaa cgcctggtac
      241 cgctcgttgt tctcaggaat ggagtcgggc cattgctgca gtaacgccgc ttttaccgcg
      301 gatgccgaag ctcccggtga cagccgatac acggacagga cccaacctaa gtgcgagagc
      361 gtccggcgcc gcagcagctg cgctccctgc gattgcatcc acgctcgttg cgcatcggac
      421 tccgcgacat cctgagatgc caccagtagc tcggccttca gggcattcgc cggcttcgcg
      481 gccggtgctt cgggcacgcc ggggtcgctc aaggcggccg gcggggccga gttggtcgac
      541 tccggattcg gctccgcgct ccgctcctct gccgcgggtt cgcgatcgcg cgggggtggc
      601 gtgcgtgtcg gtgcgacgag gtcgcggctc ctgtccggtg gtggcggcgg tggcggcggg
      661 cgcggcgccg tggtcacggt atcggtggga ccgcgccgcg gcttcacggt ggaactgctc
      721 gactgcgacc gcgctacgtc cgcccatagc agcagagccg cgaggacgca caagccagtt
      781 gcgccgcggg tgctcatggc atcacgacgg cctgtagcag cagcggctcg gcctgaagcg
      841 tcgcgcgcac agcagcatca ccgtcacccg gtcgacgcgg caggatgatt tcgtagattc
      901 ccagcgcgga cggtccgcgt atcacgcgcg cgtcgatgcg caggagcagt gcattgatat
      961 ccgcgaccgt cgcagtctcc acgaatcgca cctgcactcg ccaggaatcc gcgggcacag
     1021 ccgtggtgcc tgacaaaggc gcgtagcgat ccgcgtcgtc ctgccgcatc aggatcgcgg
     1081 tctgcagacc ggcaatcagc aacacgctcg ccgccgcggt gattcgccag agtcgcatcg
     1141 agtcgcgggc tttcacgcgc ccgagttctc gacgaaatcc gcgccagccg agctcggtgc
     1201 ccggatccgc caggggctgc gtacgcgtct gcaactggac cgcgcgccac atctgcaact
     1261 ggggctcggc atccggagat tcacgcagga acttctcgac ctgcgcgcgt tcggcatcgg
     1321 acagcgttcc gttcaggtac cacggaatga gcgcgctcac gtcgaaggat gcggcagtca
     1381 tgttgaacct cgcgcaaaca gtccggccag gcagtgcagc agccgctgct tggcgtgcat
     1441 gacgcgggtc ttgatggtgc ccaccggcac atcgagcacc tgcgccacct ccgggtatga
     1501 gagctcttcg aagaacgcca ggtgcaccac ggctttctgc cgctcgggca gttgttcgac
     1561 acagctgcgt acatgacgcg cgtcctgcgc gccgccaatc acgtcggaga tgctgcaggc
     1621 cggcgcgtcg tcggcgacgt cgtccagctc ctcggcatgt tcgtggcggc gccggcggcg
     1681 ccgcaacaga tcgatggcgc ggtgattgac gatgccaaac aaccaggtcc ggacgcgtga
     1741 atcgccggca aaccgcgcgg ccgcgcgcca cacttccatc atggtctcat tggcgagttc
     1801 ggccgcatcg gccgcgttgt tcacgagtcg cagcgcgaac tgaaagacct gcccgtggta
     1861 gtggcgataa aactgctcga gcgcggcctc ttcgccgagc gcgatccgct ctatccaccg
     1921 gcgcgactgc gcttcggcat cggcgaggct gggtgagagt gtggcgttca tggcccgcct
     1981 cactgccgca ggacgaacgg cgcgagatca ctgcgcgcga tgagccgtcc gtcgaggtcg
     2041 tgcgccgtca cgcgccacag gtagcggtga ccggacacga gcttgcgcag ggccagttcg
     2101 ccgagcggcg cggaagccga attgccgggc agcaggagcc cggtgacgaa ttcgggatcg
     2161 gcgccctgaa ccgttgccgg ctcgaagatc tgcaactggt aggtcgtcgt ccctttgacg
     2221 gcgggccagc gcagcggctc actggcgccg agatcctggt tgtcgggcca cacgccgcga
     2281 atcaccgcgg ccgcctcgcc cggctccacc tggtagttag tctgcacggt cgaggcgctg
     2341 tccgcgcatg acagcagggg caccgcgccg tcctcgatgc agaagcgcag cgtgtagcgg
     2401 ccgctcatct
//
LOCUS       DATDEU010000564         5784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1133960, whole genome shotgun sequence.
ACCESSION   DATDEU010000564 DATDEU010000000
VERSION     DATDEU010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133960"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1585
                     /locus_tag="VIV63_13435"
     CDS             113..1585
                     /locus_tag="VIV63_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY6125648.1"
                     /translation="MAMHLKRKCEQGNMPAATLVLAALLPGASLAAEGGSSNYWQGSY
                     GDFAAAIAPVPGGYVRNDVVYYDAGIGAHPISGTYDPGFDQKLWMDRLTVAGFADGGL
                     FGGKFGIELQLPYVFDLTVAQQPGGIPLGAPRDQAMGDPVLKPQLLWGSGPHYSKLSL
                     GIVIPWGTNDEDKVTTIGRDYWSFDPSFTYTFLSDSGWDFSATAGYMMNLEHSLPGTN
                     YQTGDEIHVDALFGKHFGDKFAFGIAGYWYDQMTDDDGNIPPFNDSGYKSSGAGFGPA
                     FKFGNKHFSIVGKWLYDAHAENRLDGDLYMLSFVAHFGGEPPPPPMPIVPAAPPPQPA
                     PPPPPPPPPPADADGDRVTDLSDLCPATPRGDRVDVNGCSCDVTVQVNFKSNSTEITG
                     ADAILLDSLADQVRRLPTIVGELGGHTDSQGVEAFNVELSRRRALAARDYLASRGLNV
                     TQLAIAGYGESQPISDNTTAEGRALNRRVVMRRTNCQVGP"
     gene            complement(1601..2527)
                     /gene="ppk2"
                     /locus_tag="VIV63_13440"
     CDS             complement(1601..2527)
                     /gene="ppk2"
                     /locus_tag="VIV63_13440"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY6125649.1"
                     /translation="MSAQPPLDTEQLIQRIRRDLLDSWDEEIEMDVEDYHPLPELPDG
                     GAHGAGEKEYRRNYFQQLFRLQAELVKLQDWVYATKQKVVILFEGRDAAGKGGAIKRI
                     TQKLNPRVCRVVALPAPNDRERTQWYFQRYVSHLPAAGEIVLFDRSWYNRAGVEHVMG
                     FCTDEEYEDFYRSVPEFERMLVRSGIRLLKYWFSITDEEQHVRFLGRIHDPLKQWKLS
                     PMDLESRRRWEDYTRAKEIMLERSHIPEARWWVVQAVDKKRARLNCIDHLLRQFPYAE
                     VPKQGDELPERHRHEHYSRQAVPPEMLVPEIY"
     gene            complement(2607..3110)
                     /locus_tag="VIV63_13445"
     CDS             complement(2607..3110)
                     /locus_tag="VIV63_13445"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY6125650.1"
                     /translation="MTEAEFKLESELWKALKSDRTVMLGLAGVEEGHSQPMTAQTLHD
                     DDARGPIWFFTSKDTDLAKSLTESHRAVAHFASKDHELFASLHGELVIDNDRRIIDQL
                     WNSFIAAWFQGGKDDPKLLLLRLDPERAQIWLNENNLWAAVKLLMGKDPKTDYQDKVA
                     DVRLSSH"
     gene            complement(3277..3606)
                     /locus_tag="VIV63_13450"
     CDS             complement(3277..3606)
                     /locus_tag="VIV63_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEY6125651.1"
                     /translation="MKSIKFLLPVAALALSGVAAAGSRDVASVVVRYGDLNLNSRAGV
                     VGLHKRLRNAAESVCGNLDSRILGLSDAFDQCVDEALANGVAAVGNPNLSDFHASKGK
                     GAVVASN"
     gene            complement(3711..4679)
                     /locus_tag="VIV63_13455"
     CDS             complement(3711..4679)
                     /locus_tag="VIV63_13455"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6125652.1"
                     /translation="MDALSDVLRAVRLSGAFFFDVHACAPWCAETPFGKTVVEAMFPG
                     SDHLICYHLLMEGSCWATLEGEEPIKLVAGDIIVLPQGDTHVLATAPGMRNTPEMSLY
                     RRPSDGKLPSTISVGSDGGEPAHFVCGFLGCDSRPYNPLLTALPRVIVIHNHAGGALG
                     AYFRAALAESKSSRMGSADLLGRISELMFVDVVRQYLESQPADRTNWLSGLRDPYVGR
                     ALMALHANPARDWTLEALAQEAALSRSSFAERFTQFVGHPPMQYLTNWRMQLATNYLR
                     KGTESVAAIANRVGYESEAAFSRAFKKVVGAPPSEWREQRLTGNAA"
     gene            4817..5410
                     /locus_tag="VIV63_13460"
     CDS             4817..5410
                     /locus_tag="VIV63_13460"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEY6125653.1"
                     /translation="MKAFCAVTAMTMAGSSVAATTAEPAPETHRYLIERTFPPGALDG
                     VDAAAKQKVNANNATLNVTWEKSYANADKTKTYCVYDGPSEAAVREAAKLSGMPVDNV
                     TEIPADIKAEPRGAVQHIAAGNHRYLVKRSGSPDVKAASDRKFGVTLLTSYATADKQD
                     SFWVYEAPSFSAVEEAAKASGAPFESIAEIPETLYPH"
     gene            complement(5467..>5784)
                     /locus_tag="VIV63_13465"
     CDS             complement(5467..>5784)
                     /locus_tag="VIV63_13465"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEY6125654.1"
                     /translation="NSVILRSWFMPFFLSTTLGCAVLAIAGIVSADDPRRGLMMGGGA
                     LYVAGMFLVTLLFNVPLNNALASAGNENWQKYITQWTRWNHVRTFASMGSAALFIAAL
                     VQH"
BASE COUNT         1079 a         1792 c         1852 g         1061 t
ORIGIN      
        1 agcgccgcga gcggcctagg tgaaagtagt tagtgcgtcg cgcaacggtg aactagctac
       61 cggcaccaag gtcccatatt cacccggatt tcgggcatgc gagcatgcac ccatggccat
      121 gcatctcaaa cggaagtgcg agcagggcaa tatgcccgcc gcaacactgg tcctggcagc
      181 cctgctgccg ggtgcttcgc tcgccgccga aggtggcagc agtaactact ggcagggatc
      241 gtacggcgac ttcgccgctg ccatcgcacc ggttccgggc ggttacgtgc gcaacgatgt
      301 cgtctattac gacgcgggca tcggcgcgca tcccatctcc ggtacctacg atcccgggtt
      361 cgaccagaaa ctgtggatgg atcggctgac ggtcgcaggt ttcgccgatg gcgggctctt
      421 cggcggaaag ttcggcatcg agctgcagct gccatacgta ttcgatctga ccgtggcgca
      481 acagccgggc ggaattccgc tgggcgcgcc acgcgaccag gcgatgggcg atccggtgct
      541 caagccgcag ctcttgtggg gatccggtcc gcactacagc aaactgtctc tcggaatcgt
      601 cattccgtgg ggcacgaacg acgaagacaa ggtcacgacc atcggccgcg actactggtc
      661 gttcgatccg tcgttcacct atacgttcct gagcgacagc ggctgggatt tctcggcgac
      721 cgccggctac atgatgaacc tcgagcacag cctgcccggg actaactacc agaccggcga
      781 cgaaatacac gtcgacgcgt tgttcggcaa acacttcggc gacaaattcg cgttcggcat
      841 cgccggctac tggtacgacc agatgaccga cgacgacggc aacatcccgc cattcaacga
      901 ttccggctac aagagctcgg gcgcgggctt cggccccgcg ttcaagttcg gcaacaagca
      961 cttcagcatc gtcggcaaat ggctgtacga cgcgcacgcg gagaaccgcc tcgacggtga
     1021 cctgtacatg ttgtcgttcg tggcgcactt tggtggtgaa ccacctccgc ctcccatgcc
     1081 catcgttccg gcagcgccgc cgccgcaacc cgctccgccg cccccaccgc cgccgccacc
     1141 gccggctgac gccgatggcg atcgcgtgac cgatctttca gacctgtgcc ccgcgacgcc
     1201 gcgcggcgat cgtgtggacg tcaacggttg ctcctgcgat gtgaccgtgc aggtgaactt
     1261 caagagcaac tccacggaga tcaccggtgc ggacgcgatc ctgctcgata gcctggcgga
     1321 ccaggtgcgc cgtctgccga ctatcgtcgg cgagttaggt ggtcacaccg acagtcaggg
     1381 agtggaagca ttcaatgtcg agctgtcgcg cagacgtgca ctcgcggcca gggactacct
     1441 ggcgtcgcgt gggctcaatg tgacgcagct tgcgatcgcc ggctacggcg aatcacagcc
     1501 gatctcggac aacaccacgg cggaaggacg cgcgctgaac cggcgcgtgg tcatgcgccg
     1561 cacgaattgc caagtggggc cgtagctcgc gcgcgggcgc tcagtagatc tcgggcacca
     1621 gcatttccgg cggcacggcc tggcgtgagt agtgctcgtg gcggtggcgc tccggcagct
     1681 cgtctccctg ttttgggacc tcggcgtacg ggaactgccg gagcaggtgg tcgatgcagt
     1741 tgagccgcgc gcgtttcttg tccacggcct gtaccaccca ccagcgtgcc tcggggatgt
     1801 ggctgcgctc gagcatgatt tccttggcgc gtgtgtagtc ttcccagcgc cggcgcgact
     1861 cgagatccat cggcgacaac ttccactgct tcagtggatc gtggatgcgc ccgaggaacc
     1921 gcacgtgctg ttcctcgtcg gtgatcgaga accagtattt caacaaccgg attcccgagc
     1981 gcaccagcat gcgttcgaat tcaggcaccg atcgatagaa gtcttcgtac tcctcgtcgg
     2041 tgcagaagcc catgacgtgt tcgacgccgg cgcggttgta ccagctgcga tcgaacagca
     2101 cgatttcgcc ggcggccggc aggtgcgaga cgtaacgctg gaaataccac tgtgtgcgtt
     2161 cgcggtcgtt gggagcgggc agggcgacca cgcggcacac gcgcgggttc agcttctgcg
     2221 tgatgcgttt gatggcgcca cccttaccgg cggcgtcgcg gccttcgaac aggatcacca
     2281 ccttctgctt ggtggcgtac acccagtcct gcagcttcac gagctccgcc tggaggcgga
     2341 acagctgctg gaagtaattg cggcggtact ccttttcgcc ggcgccatgg gcgccgccat
     2401 ccggcagctc ggggagcgga tggtagtcct cgacatccat ctcgatctcc tcgtcccagc
     2461 tgtcgaggag atcgcggcgg atacgctgga tgagttgctc ggtgtcgaga ggcggctggg
     2521 cgctcatgcg ggctccacgt acagggaccc gcgatgctcg cgccgcaatg tgacagttgt
     2581 gtgtaaatcg gtgcctggca ccgttttcag tgcgagctca ggcgcacgtc ggccaccttg
     2641 tcctggtaat ccgtcttggg atccttgccc atcaacagtt tgaccgcggc ccagaggttg
     2701 ttttcgttca accagatctg cgcgcgctcg gggtcgagtc ggagcagcag cagtttgggg
     2761 tcgtctttgc caccctggaa ccaggcggcg atgaagctgt tccagagctg atcgatgatg
     2821 cggcgatcgt tgtcgatgac cagctcaccg tgcagcgatg cgaacaactc gtggtccttg
     2881 gaggcaaagt gggccaccgc gcgatgcgat tcggtcaggg acttcgcgag gtcggtgtcc
     2941 ttcgacgtga aaaaccagat ggggccgcgc gcatcgtcgt catgcaacgt ctgcgcggtc
     3001 atgggctggc tgtgtccttc ctcgacgccg gccaatccga gcatcacggt gcgatcactt
     3061 ttgagcgctt tccacagctc gctttccagt ttgaattcgg cttcggtcat ggatagatcc
     3121 tccggtgttt gtcaccggag gatcacaccc gcgccgatcg atttgacgtg gtcaattcaa
     3181 tggatcgatg tcagcgccgg cctgcacggc tgcaaaagct gatcccgcgc gcgccttcga
     3241 actaatgcgg ccctcgcggc gatgcaggca ctattttcaa ttggacgcga caaccgcgcc
     3301 ctttcctttg ctcgcgtgga aatccgacag gttcggattg ccgacagcgg cgacgccgtt
     3361 cgccagggct tcgtccacgc attggtcgaa tgcgtcgctc aggcccagga tgcgcgagtc
     3421 cagattgccg caaaccgact cggccgcgtt gcggaggcgc ttgtgcagcc ccacgacacc
     3481 ggcgcgcgaa ttcagattga ggtcgccgta acgcacgaca acgctcgcca cgtcgcgcga
     3541 accggcggcg gcaacgccgc tcagggccaa cgctgcgacg ggtaacagaa acttgatcga
     3601 cttcatgatg tgctcctcgt tgattacagg gggcacatgc tgccgaaacg gaaatccggc
     3661 gtgttccgta gttctgacgg gatgattagt tttactgaac gatcagcctg ctaggcggca
     3721 ttgccggtca ggcgctgttc gcgccactcg ctgggcggcg cgccgaccac cttcttgaag
     3781 gcgcggctga atgcggcttc ggattcgtat cccacgcgat tggcgatggc ggcgacgctc
     3841 tcggtgccct tgcgcagata gttggtcgcg agctgcatgc gccagttggt gaggtattgc
     3901 atgggcggat ggccgacgaa ctgcgtgaaa cgctcggcga atgacgagcg cgacagcgcc
     3961 gcttcctgcg ccagcgcctc gagcgtccag tcgcgcgcgg gattggcgtg cagcgccatc
     4021 agtgcgcgtc ccacataagg atcgcgcagg cccgacagcc agttggtgcg atcggcgggt
     4081 tgcgattcga ggtattgccg gaccacgtcg acgaacatga gttcgctgat gcggcccagc
     4141 agatccgcgc tacccatgcg cgaactcttc gactccgcga gcgccgcgcg aaaataggca
     4201 cccaatgcgc cgcccgcgtg gttgtggatg acgatgacgc gcggcaacgc cgtgagcagc
     4261 gggttgtaag ggcgcgagtc gcagccgaga aagccgcaca cgaagtgcgc gggttcgccg
     4321 ccatcgctgc ccaccgagat cgtcgacggc aatttcccat cgctgggccg gcggtacagg
     4381 gacatttccg gcgtgttgcg catgccgggc gcggtcgcga gcacgtgggt gtcaccctgc
     4441 ggcagcacga tgatgtcgcc ggcgactagt ttgatcggct cttctccttc gagtgttgcc
     4501 caacaacttc cttccatgag caggtgataa cagatgaggt gatcggagcc gggaaacatg
     4561 gcctcgacga cagtcttgcc gaaaggcgtt tccgcacacc agggcgcgca ggcatgcacg
     4621 tcgaaaaaga acgcgccaga caggcgtacg gctcgcaaca catctgacaa agcgtccatg
     4681 gggcctcccc aaacttcgcc ggacgctggg ataacaacgc cggacttacg gtcattcgaa
     4741 aacactgttc gcgcagttac tttgtcagcg tggtgatgag taatcaactt cagtacaagc
     4801 atcggagatg acgatgatga aagccttttg cgccgtgacg gccatgacga tggcgggatc
     4861 gagcgtcgcc gcgacgacgg cggagcccgc cccggaaacg caccgctatc tgatcgagcg
     4921 caccttcccg ccgggcgcgc tcgatggagt cgacgccgcc gcgaaacaga aagtgaacgc
     4981 gaacaatgcg acgttgaacg tgacctggga aaagtcgtac gcgaatgcgg acaagaccaa
     5041 gacgtattgc gtgtatgacg gcccgagcga agccgcggtg cgcgaagccg cgaagttgtc
     5101 aggcatgccc gtcgacaacg tcacggaaat tcccgcggac atcaaggcgg aaccgcgcgg
     5161 cgcggtgcag cacatcgccg ccggcaatca tcgctatctt gtgaagcgtt cgggctcgcc
     5221 cgacgtgaag gccgccagcg accggaagtt cggcgtgacc ttgctcacgt cctatgccac
     5281 ggcggacaaa caggattcct tctgggtgta cgaagcgccg agtttctcgg cagtggaaga
     5341 agcggcgaag gcgagcggtg cgccgttcga atcgatcgcg gagattcccg agaccctgta
     5401 cccgcactga ggtgttgtca ggcgcgggtg ctggagcggg cggtggcgcg agccaccgcc
     5461 caaacctcag tgttgcacga gggcggcgat gaacagtgcg gccgagccca tcgacgcgaa
     5521 ggtgcgcacg tgattccagc gcgtccattg cgtgatgtac ttctgccagt tctcattgcc
     5581 tgcggatgcc agcgcgttgt tgagcggcac gttgaacagc agcgtgacga ggaacatgcc
     5641 ggccacataa agggcgccgc cgcccatcat gagaccgcgt cgcggatcgt cagcactgac
     5701 gatgccggcg atcgccagca ccgcgcagcc gagcgtggtg ctcaggaaaa atggcatgaa
     5761 ccacgagcgc aggatgactg aatt
//
LOCUS       DATDEU010000565         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_401748, whole genome shotgun sequence.
ACCESSION   DATDEU010000565 DATDEU010000000
VERSION     DATDEU010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401748"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /gene="infA"
                     /locus_tag="VIV63_13470"
     CDS             <1..191
                     /gene="infA"
                     /locus_tag="VIV63_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY6125655.1"
                     /translation="GEVVETLPNTTFRVKLKTGHVVTAHISGKMRKNYIRILTGDQVT
                     VEMTPYDLSKGRIVYRGR"
     gene            202..1104
                     /locus_tag="VIV63_13475"
     CDS             202..1104
                     /locus_tag="VIV63_13475"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY6125656.1"
                     /translation="MAASRRSQAEYDRRMHAVVDYIDRNLDRKLDLAEIASVASFSPF
                     HFHRLFRALAGEALGDYVRRRRLESGAVRLRAQERISVLSVAVSVGFGSAEAFTRAFR
                     ARFGCSPTQWRKSKHDQTMRKAGQAGKKPARKNGGSRIKESAMNVKIIDRPPVHVAYL
                     RHTGAYGPQIGRFWMETVAPWMATNNLFGRDRFGIGLDDVSVTKPSQCRYDACVASPE
                     SEVLTGNAHRKVIPGGKYAALPFEGTSAEIGAAWESLLRDWLPKSGLQLDARPFFEHY
                     PVDGRYDPKSGRFTCNICVPVASL"
     gene            complement(1105..>3049)
                     /locus_tag="VIV63_13480"
     CDS             complement(1105..>3049)
                     /locus_tag="VIV63_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6125657.1"
                     /translation="AIFSEKQSHAVHLLNRAEVTRLDVVNFVSHGLSKIAEEKTAKEE
                     PGGEGERDADGGSALDKYTANLNQLAQDGKIDPLIGRTLEVERTIEILCRRRKNNPLY
                     VGEAGVGKTAIAEGLARLIVEGKVPDVLSDCTIYSLDMGSLIAGTKYRGDFEKRLKSV
                     IGELKKQPGAILFIDEIHTVIGAGAASGGVMDASNLIKPVLTNGELRCIGSTTYQEYR
                     GIFEKDHALARRFQKIDVVEPTVSETIDILMGLKPRFEQHHGITYTHDAIRAAAELAA
                     RHINERHMPDKAIDVVDEAGARLRLKPVNERESSVEVRHIEDVVARMARIPAKSVSTS
                     DKEVLRNLERNLKLVIYGQDKAIDSLAAAIKMARSGLGDQRKPVGSFLFAGPTGVGKT
                     EVTRQLAIALGIEFIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAIAKHPHCV
                     LLLDEVEKAHPEVFNLLLQVMDHGTLTDNNGRKADFRHVIIVMTTNAGAYEMSRSSIG
                     FVHADNASDGAEAIKRLFSPEFRNRIDSTIQFAPLSMPVIERVVDKLLVEIEAQLEKK
                     GVSILLDDAARRWLAEKGYDPKMGARPMARLMQDQIKRPLAEELLFGKLAVGGTVKIG
                     VSSDGSELTLTTLPAETPALTVQ"
BASE COUNT          515 a          955 c          977 g          602 t
ORIGIN      
        1 aaggcgaagt cgtcgagact ttgcctaaca ctactttccg cgtgaagctg aagactggtc
       61 acgtcgtgac cgctcatatt tcaggaaaga tgcgcaagaa ctacatccgc attctcacgg
      121 gcgaccaggt caccgtcgag atgacgccct acgatttgtc caagggacgc atcgtctacc
      181 gcggcagata acaccgcgag cgtggccgcc tcccgccgca gccaggccga atacgaccgg
      241 cgaatgcacg cggtggtcga ttacatcgac cggaatctcg atcgaaaact cgacctcgcg
      301 gaaatcgcga gcgtcgccag tttttcgccg ttccatttcc accgactgtt tcgcgccctg
      361 gcgggcgaag cgctgggcga ctacgtccgg cgccgccggc tcgaatccgg ggcagtaagg
      421 ctgcgtgcgc aggaacgtat atccgtgctc agcgtcgcgg tcagcgtcgg cttcggttcc
      481 gccgaagcgt tcacgcgcgc gtttcgcgcg cgcttcggct gctcgcccac gcagtggcga
      541 aagagcaagc acgatcagac gatgcgcaag gcaggtcagg ccggaaaaaa accggcgcga
      601 aagaatggcg gctcccgaat caaggagagc gccatgaatg tgaagatcat cgatcgcccg
      661 cccgtccacg ttgcctatct tcgtcacacc ggcgcttatg gcccgcagat cggccggttc
      721 tggatggaga ccgtcgcgcc gtggatggcc accaacaacc tgttcggccg cgatcgcttc
      781 ggcatcggtc tcgacgatgt cagcgtcacc aagccatcgc agtgccggta cgacgcctgc
      841 gtggccagtc ccgaaagtga agtgttgacg ggcaatgccc atcgcaaggt gattccgggc
      901 ggcaaatacg ccgcgttgcc tttcgagggc accagcgccg aaatcggcgc ggcctgggaa
      961 tcgttgctgc gagactggtt gccgaagagc ggactgcaac tcgatgcgcg tccgttcttc
     1021 gagcactacc cggtcgatgg gcgttatgac ccgaagtccg gccgcttcac ctgcaacatc
     1081 tgcgtgccgg tggccagtct gtagttactg gacggtgagc gccggcgttt ccgccggcag
     1141 cgtcgtcagc gtgagttcac tgccgtcgct gctcacgccg atcttgaccg tgccgcccac
     1201 cgcgagcttg ccgaacagca gctcttcggc gagcggccgc ttgatctgat cctgcatgag
     1261 ccgcgccatc gggcgagcac ccatcttcgg gtcgtagccc ttctccgcca gccagcggcg
     1321 cgccgcgtcg tcgagcagga tgctcacgcc cttcttctcg agctgcgctt cgatctcgac
     1381 cagtagtttg tcgaccacgc gttcgatgac cggcatgctg agcggcgcga actggatcgt
     1441 ggagtcgatt cgattgcgga actcgggcga gaacaggcgc ttgatggctt ccgcgccgtc
     1501 ggacgcgttg tcggcatgca cgaagccgat ggacgaccgg ctcatctcgt aggcgcccgc
     1561 gttcgtggtc atgacgatga tcacgtggcg gaaatcggcc ttgcgtccgt tgttgtcggt
     1621 gagcgtgccg tggtccatga cctgcagcag caggttgaac acttcgggat gcgccttctc
     1681 gacctcgtcg agcagcagca cgcaatgcgg atgtttggcg atggcttcgg tgagcagtcc
     1741 gccctggtcg aatccgacgt agcccggagg cgcgccgatg agacgcgata ccgtgtgccg
     1801 ctccatgtac tcggacatgt cgaagcggat gaattcgatg ccgagcgcga tggcgagctg
     1861 gcgcgtgact tcggtcttgc cgacgcccgt gggaccggca aacaggaagc ttccgaccgg
     1921 cttgcgctga tcgccgaggc ccgagcgcgc catcttgatc gccgcggcca gcgagtcgat
     1981 ggccttgtcc tgaccgtaga tgaccagctt gagattgcgc tcgaggttgc gcagcacctc
     2041 tttgtcggac gtggacacgc tcttggcggg gatacgcgcc atgcgggcga cgacatcttc
     2101 gatgtgtctc acttcgaccg acgattcacg ttcgttgacg ggtttcaggc gcaggcgcgc
     2161 accggcctcg tcgacgacgt cgatggcctt gtccggcatg tgccgttcgt tgatgtggcg
     2221 cgcggcaagc tccgccgcgg cgcgaatggc gtcatgcgtg taggtgatgc cgtggtgctg
     2281 ctcgaaacgc ggcttcaggc ccatgaggat gtcgatggtt tccgagaccg tgggctcgac
     2341 cacgtcgatc ttctggaagc ggcgcgcgag cgcgtggtcc ttctcgaaga tgccgcggta
     2401 ttcctggtag gtagtcgagc cgatacaccg cagttcgccg ttggtgagca ccggcttgat
     2461 gaggttcgag gcatccatga cgccgcccga cgccgcgccg gcgccgatga cggtgtggat
     2521 ctcgtcgatg aacaggatgg cgcccggctg cttcttgagc tcgccgatca cgctcttgag
     2581 ccgcttctcg aaatcgccgc gatacttggt gccggcgatg agcgagccca tgtcgagcga
     2641 atagatagtg cagtccgaca gcacgtcggg cacttttcct tcgacgatca agcgagccag
     2701 gccctccgca atggccgttt ttccgacacc cgcttcgccc acatacagcg gattgttctt
     2761 gcggcgccgg cacagaatct cgatggtgcg ttccacctcg agcgtgcggc cgatcaacgg
     2821 gtcgatcttg ccgtcctgcg cgagctggtt gaggttggcg gtgtacttgt cgagcgcact
     2881 gccgccgtcg gcgtcgcgtt caccctcgcc gcccggctct tctttcgcgg ttttctcttc
     2941 ggcgatcttc gagaggccgt gcgagacgaa attgaccacg tcgaggcgcg tgacctcggc
     3001 gcgattcagc aggtgcaccg catggctctg cttttcgctg aagatggcg
//
LOCUS       DATDEU010000566         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_551028, whole genome shotgun sequence.
ACCESSION   DATDEU010000566 DATDEU010000000
VERSION     DATDEU010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551028"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VIV63_13485"
     CDS             complement(<1..1479)
                     /locus_tag="VIV63_13485"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEY6125658.1"
                     /translation="MNEIAREILPVNLEDEMRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRILHAMRELGNDWNKAYKKSARVVGDVIGKYHPHGDNAVYDALVRMAQPFSMRYLL
                     VDGQGNFGSVDGDAPAAMRYTEVRMSRIASELLADIEKETVDFQPNYDESETEPTVMP
                     ARFPNLLVNGSAGIAVGMATNVPPHNLTEIINACLAVLDDGEVTLAQLMQHVPGPDFP
                     TAGIINGATEIATAYRTGRGRLSIRARCNFEDIDKVGGRQAIIVTELPYQVNKARLLE
                     RIAELVRNKEIDGISELRDESDKDGMRVVIELKRGEVSEVVLNNLFAQTPLETVFGIN
                     MVALIDGRPKLLGLKEMLEAFLRHRREIVTRRTIFDLAKARDRAHILEGLAVALANID
                     EIIALIKSSPTPPEAKAALAAREWSSGVVPQLLARAGDISTRPQGLAAEFGIVGDGGV
                     RYKLSEIQAQAILDMRLHRLTGLEQDKIVAEYQDLLATIVDLT"
     gene            1594..>2061
                     /gene="ubiG"
                     /locus_tag="VIV63_13490"
     CDS             1594..>2061
                     /gene="ubiG"
                     /locus_tag="VIV63_13490"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY6125659.1"
                     /translation="MTTTQTSGNSPHDSLNSDAAEIAKFDASAHRFWDVDGEFKPLHK
                     LNPVRARYVAERCTLAGSSVLDVGCGGGLLAESLARAGAKVTAIDLAPSMVETARLHA
                     LDSGLTIDYRVESAEQLLKAPGKFDVVTCMEMLEHVPDPGATVAVLGKLLRPGG"
BASE COUNT          350 a          633 c          681 g          397 t
ORIGIN      
        1 ggtgagatcg acgatggtcg cgagcagatc ctgatattca gcgacgatct tgtcctgctc
       61 gaggcccgtg agccggtgca ggcgcatgtc gagaatcgcc tgagcctgga tttccgaaag
      121 tttgtagcgc acgcccccat cgcccacgat gccgaattcg gccgcgaggc cctgaggacg
      181 ggtgctgatg tcgccggcgc gagccagcag ctgcggaact actcccgagc tccactctcg
      241 cgcggccagc gctgccttgg cttccggcgg cgtcggagac gatttgatga gcgcgatgat
      301 ctcgtcgatg ttggcgagcg cgaccgcgag gccctcgaga atgtgggcgc ggtcacgggc
      361 cttggcgaga tcgaaaatgg tccggcgcgt gacgatttcg cgacggtgcc gaaggaatgc
      421 ttcgagcatt tccttgaggc cgagcaactt cggccggccg tcgatgagcg cgaccatgtt
      481 gatgccgaac accgtctcga gcggcgtctg cgcgaagagg ttgttgagca cgacctcgga
      541 gacttcgccg cgcttgagct cgatgaccac gcgcatgccg tccttgtcgg actcgtcgcg
      601 cagttcggag atgccgtcga tttccttgtt gcgaaccagc tcggcgatac gctcgagcag
      661 gcgcgccttg ttcacctggt acggcagctc ggtgacgatg atggcctggc ggccgcccac
      721 cttgtcgatg tcttcgaaat tgcagcgcgc gcgaatggac aggcggccgc gaccggtgcg
      781 ataggccgtg gcgatttcgg ttgcgccatt gatgatgccg gcggtcggga aatccggacc
      841 cggcacgtgc tgcatgagct gcgccagcgt gacttcgccg tcatcgagca ccgcgaggca
      901 ggcgttgatg atttccgtga ggttgtgcgg cggcacgttg gtggccatgc ccaccgcgat
      961 gccggccgag ccgttgacca ggaggttggg gaaccgcgcg ggcatgaccg tgggttctgt
     1021 ttcgctctcg tcgtagttcg gctggaaatc gacggtttct ttttcgatgt cggcgagcag
     1081 ctcgctcgcg atgcgcgaca tacgcacttc ggtgtaacgc atggccgccg gcgcgtcgcc
     1141 gtcgaccgag ccgaagttgc cttggccgtc gaccagcagg taacgcatgg aaaacggctg
     1201 cgccatgcgc accagcgcgt cgtacacggc gttgtcgccg tgcgggtggt atttaccgat
     1261 gacgtcgccg acgacgcgcg cggacttctt gtaggccttg ttccagtcgt taccgagctc
     1321 gcgcatggcg tgcaaaatgc gccgatgcac gggcttcagg ccgtcgcgca cgtcgggcag
     1381 cgcgcggccg acgatgacgc tcatggcgta gtcgagatac gactggcgca tctcgtcttc
     1441 gagattcacg ggaagaattt cgcgcgcgat ttcgttcatg gaagagtggt ggctgggccc
     1501 gaaaaaaggt gcggaattct atcacgtagg cctaaccaag aacccggttt taacgcgctt
     1561 tacacttgcc gcgcgtctat aattgctgcc ctcatgacga ccacccagac atccggtaat
     1621 tcgccccacg attcgctgaa cagcgacgcc gcggaaatcg cgaaattcga cgcgtcggcg
     1681 catcgcttct gggatgtcga cggtgagttc aagccgctgc acaagctcaa tcccgtgcgc
     1741 gcgcgttacg tcgcggagcg ctgcacgctc gcgggatcca gtgttctcga cgtcggatgc
     1801 ggcggtggat tactcgccga atccctcgcg cgcgcgggcg ccaaggtcac ggccatcgat
     1861 ctcgcgccga gcatggtcga aacggcgcgc ctgcatgcgc tcgattccgg tctcaccatc
     1921 gactaccgcg tcgagtccgc cgagcaattg ctgaaagccc cgggaaaatt cgacgtcgtc
     1981 acctgcatgg agatgctcga acacgtgccc gatccgggcg ccaccgtcgc ggtgctcggc
     2041 aaactgctgc ggccgggcgg c
//
LOCUS       DATDEU010000567         4559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_695402, whole genome shotgun sequence.
ACCESSION   DATDEU010000567 DATDEU010000000
VERSION     DATDEU010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695402"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VIV63_13495"
     CDS             <1..282
                     /locus_tag="VIV63_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125660.1"
                     /translation="VKEERPFGFFVSIGLVLAAVSVVLAIPLFIEFFATGLVPRIPTA
                     VLSTGLMLMAMMSFVCGLILDTVTRGRRELKRMHYLMHTQAGPASENSR"
     gene            279..2081
                     /locus_tag="VIV63_13500"
     CDS             279..2081
                     /locus_tag="VIV63_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125661.1"
                     /translation="MTPAFRWTLLWIAGSIVMVAGALAMLTASLSDNQYLPANADAFY
                     HARRILDSVFSGNPVIQFDDRIHVPEGSWLTWPWGFDTVMARITSLFGPFANRDDASR
                     VLMNIPPAAAPIAVALVVNIARQLKLNFVFAALFVLGFAALPVAHGLFGVGNVDHHFA
                     ELLWTLGTLSAGIWFFRPGAPSYASGIVLGCVLGTSLSIHNSLFILQIPVVLTLTLFW
                     LQAGTFPDRPRMLGFAGALLVSTTLMCIPSEPWRRGFFEFYTLSWFHFYISACVAVFS
                     VLLSWLPRSGRNIAIIGLAAGAALIPLFGALSFGSEFISGELASIRNITEANSPYVMY
                     RTYGEAFSTRLLSWMMWLTGPMLLLNLWWVFRLRESELRFVALAGVLGLALMQFQFRF
                     SAFGEASMLLTPLLLAGFIADRLPERRIEALLASVLLFGFAFYPTLTNWKTRWQLSGD
                     RAYVWLRSALPALKTACDERRGVVLADIDAGHWIRYHSECSVIGDVFLLTPLHGAKAL
                     ESARLLRLSPTELVAESRDIRYVFARHSTMMFLDRGGREGPDLNELRGKLPALERELL
                     APDATLPPQYRMLNEARTPSGQVYARLYEIDREP"
     gene            2078..2791
                     /locus_tag="VIV63_13505"
     CDS             2078..2791
                     /locus_tag="VIV63_13505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6125662.1"
                     /translation="MKRERVNFIRYLLEEWIPPALRDSAPMRWLFRAYWGRFVDDLEK
                     FRANIHRVTPAEYADIYRRMPRIQESTDNSDACIRRLVELMLPGSVCDVGCGTGHLLG
                     VLASNSKGVPRKFAGVDFQLEPEISQRHPGVEFREAGVEHLPFPDRAFDTVICTHVLE
                     HVLRIDLTILELRRITARRLLIVVPLEREYRFTFNPHVHFFPYPHSFLRHISPVPSNA
                     RCEVIGRDLLYIEDVLEGS"
     gene            2788..3147
                     /locus_tag="VIV63_13510"
     CDS             2788..3147
                     /locus_tag="VIV63_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125663.1"
                     /translation="MNWTPLVWLELLCFPVAIAAGQILFKRAAAQMTGAGGPWLLALA
                     KLPSMWLALALYAVSTLLWVKILTTVPLSRAYPFVALAFILVPAAGYLFFDEPINTRL
                     AAGTALIIAGVIVAVRA"
     gene            3187..3597
                     /locus_tag="VIV63_13515"
     CDS             3187..3597
                     /locus_tag="VIV63_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY6125664.1"
                     /translation="MPFYEYQCPHCGYDEEVLQAINDKPLTKCPSCGKKGLKKLMSAP
                     VFRLKGSGWYETDFKGDKENKRNLAGADKDEPKAEAKTDTKSDAKVEAKADAAKPEKA
                     EAAKPAPEKASKSASKPVAKKAKSKAKPARKARR"
     gene            3602..>4559
                     /locus_tag="VIV63_13520"
     CDS             3602..>4559
                     /locus_tag="VIV63_13520"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEY6125665.1"
                     /translation="MNYARRPGINSEALTRLAARERETFKTTHVESERLAKLSAKHWL
                     NGVPMHWMRDWSTPHPLFVRKARGIEVEDVDGNTYLDFCFGDTGSMFGHSPPAIARAL
                     AETGAMGITSMLPADRVARVGEKLAKMFGLPFWQMTQTATDANRAALRWARAITGRQR
                     MLVFDGCYHGTVEETLVRALPDMNETGGTRARAGLIGMNHDVSVTTDAVPFNDIAAVE
                     RVLKRTRTAAVIAEPVMTNIGMVLPEEGFLQQLREVTRRLHVLLIIDETHTLSTARGG
                     YARAQKIEPDIWVCGKAIAGGMPCAVFGFTAEVEAGMRRVQED"
BASE COUNT          802 a         1451 c         1384 g          922 t
ORIGIN      
        1 gtgaaggaag aacgcccttt cggattcttc gtcagcatcg gcctcgttct ggccgcagtc
       61 tcggtagtcc tcgccatccc gttgttcatc gagttctttg cgaccggtct cgtgccccgc
      121 attcccaccg cagtgctttc cacagggctc atgctcatgg cgatgatgag tttcgtctgc
      181 ggcctcattc tcgacaccgt gacgcgcgga cgccgcgagc tcaagcgtat gcactacctc
      241 atgcataccc aggcgggccc cgcgtcagag aattctcgat gacccctgcg tttcgctgga
      301 cgctgctctg gattgcggga tccattgtca tggtggcggg ggcgctcgcc atgctgaccg
      361 caagcctgtc cgataaccaa tacctgcccg ccaacgccga tgccttctac cacgcgcgtc
      421 ggattctgga ttccgtattc agtggaaacc cggtcattca gttcgacgac cgtatccatg
      481 ttccggaagg cagctggctc acgtggccct ggggcttcga caccgtgatg gcgcggatca
      541 ccagcctctt cgggcctttc gcaaaccgtg acgacgcaag ccgcgtgctc atgaatattc
      601 ctccggccgc cgcgcctatc gccgtggcgc tggtcgtcaa tatcgcccgc caactcaagc
      661 tgaattttgt gttcgcggcg ctgttcgtcc tgggcttcgc tgcactgccg gtggcgcatg
      721 ggttgttcgg ggtcggcaac gtcgatcatc acttcgcgga attgctatgg acgcttggaa
      781 ccctgagcgc gggcatctgg ttcttccgtc ccggtgcgcc ttcttacgct tccggcatcg
      841 tgctcggatg cgtgctcggt acttcgctct ccatccacaa cagcctgttc atcctgcaga
      901 ttccggtcgt gctcacgttg acgctgttct ggctgcaggc cggaacattc cccgatcgcc
      961 cacgcatgct cggtttcgcg ggtgcactgt tggtctccac cacgcttatg tgtattccgt
     1021 cggagccgtg gcgccgcggc ttcttcgagt tctacacgct gtcgtggttt cacttctaca
     1081 tctcggcctg cgtggcggtc ttcagcgtgc tgctgtcctg gttgcccaga agcggacgga
     1141 acatcgcaat catcggcttg gcggccggcg ctgctctgat cccgctattc ggggcactct
     1201 ccttcggcag cgagttcata tccggcgaac tggcgagcat tcgcaacatc acagaggcca
     1261 atagcccgta cgtgatgtat cgcacttacg gtgaggcgtt ctcgacgcga ctgctgtcct
     1321 ggatgatgtg gctcaccggt cccatgttgt tgttgaacct gtggtgggtg ttccgcttgc
     1381 gcgaaagtga attgcggttc gtcgcattgg cgggagtcct cggtcttgcc ctcatgcagt
     1441 ttcaattccg cttctcggcg ttcggtgagg cgtcgatgtt gcttacacct ttgctgctcg
     1501 cgggtttcat cgcggatcgg ctgcccgaga ggcgcatcga ggccttgctc gccagcgtcc
     1561 tgttgttcgg cttcgcgttc tacccgaccc tcaccaattg gaaaacgcgc tggcagttga
     1621 gcggcgatcg ggcctatgtc tggttgcggt ccgcactccc ggctctgaag acggcttgcg
     1681 atgaacgccg cggagtggtg ctggccgaca tcgatgcagg gcactggatc cgctaccact
     1741 ccgaatgctc ggtcatcggc gacgtattcc tgcttacacc acttcatggc gccaaggcac
     1801 tcgagagcgc gcggctgctg cggctctcgc ccacggaact cgtcgcggaa tcccgggata
     1861 tccgctacgt attcgcgcgg cattccacga tgatgttcct cgacagaggc ggtcgggaag
     1921 gcccggatct caacgaactg cgcggcaaac tgcctgctct tgaacgtgag ttgctggcgc
     1981 ccgatgcaac actgcctccg caataccgca tgctgaacga agcgcggacg ccgagcgggc
     2041 aagtgtatgc acggctgtac gaaatcgatc gcgagccttg aagcgcgaac gagtcaattt
     2101 catccgttat ctgctcgagg agtggattcc gcctgcatta cgggattcgg cgccgatgcg
     2161 gtggctgttc cgcgcctatt ggggccgctt cgtcgacgac ctcgaaaagt ttcgcgccaa
     2221 tatccatcgc gtgacgcccg cggaatacgc cgacatctat cggcgcatgc cacgcatcca
     2281 ggaaagcacc gacaacagcg acgcatgcat ccgccgcctc gtcgaactaa tgctccccgg
     2341 ttctgtttgt gacgtcggct gcggcacggg gcatctgctg ggcgtactcg cctcgaattc
     2401 gaaaggtgtt ccgcgcaagt tcgccggtgt ggattttcag ctcgaacctg agatctccca
     2461 gcggcatccg ggcgttgagt ttcgggaagc gggcgtcgag catctaccgt ttccggaccg
     2521 tgctttcgat acggtcattt gcacgcacgt gctcgagcac gtcttgcgca tcgaccttac
     2581 gatcctcgaa ttacgccgaa ttaccgctcg ccgactgctg atcgtcgtgc cgctggaacg
     2641 cgagtaccgg ttcactttca atccacacgt gcacttcttc ccttatcccc acagtttcct
     2701 tcggcacatt tcaccggtgc cttcgaatgc ccgttgcgaa gtcatcggcc gcgaccttct
     2761 gtatatcgaa gatgtgttgg agggctcatg aactggacgc cgctggtttg gctcgagctg
     2821 ctgtgctttc cggtggctat cgccgcgggt cagattctct tcaaacgcgc cgcggcgcag
     2881 atgaccgggg caggcgggcc gtggctgctg gcactcgcga agctgccttc catgtggctc
     2941 gccctggcgc tttatgcggt gtcgacattg ctgtgggtga agattctgac caccgtgccc
     3001 ctgagccgcg cgtacccgtt cgtggcactg gcattcatcc tggtaccagc agccggttac
     3061 ctcttcttcg atgagccgat aaacaccaga cttgcagcag gaacggcgct gattatcgcc
     3121 ggggtcattg tcgccgtacg cgcatgacgt ccataatccg ccgcgtttcc agcgtccgga
     3181 cgactcatgc ctttctacga ataccaatgt ccccactgcg gctacgacga agaagtactg
     3241 caggcgatca atgacaagcc gctcaccaag tgcccgagct gcggcaagaa agggctgaag
     3301 aagctcatgt ccgcgcccgt gttccgcctg aaaggctcgg gctggtacga aacggacttc
     3361 aagggcgaca aggaaaacaa acgcaacctg gccggcgctg acaaggacga gcccaaggcc
     3421 gaggcgaaga ccgacaccaa gagcgatgcg aaggtcgaag ccaaggcgga cgccgccaag
     3481 cccgaaaagg ccgaggctgc gaagcccgcg cccgaaaagg ccagcaagag cgcgagcaag
     3541 ccggtggcca aaaaggccaa gtccaaagcc aaacccgccc ggaaggcgcg cagataagcc
     3601 cgtgaattac gcgcgtcgcc ctggcatcaa cagcgaagcg ctgaccaggc tcgcggcgcg
     3661 cgaacgcgaa acgttcaaga cgactcacgt cgaatccgag cggctcgcga aactcagcgc
     3721 gaagcactgg ctcaacggcg tgcccatgca ctggatgcgc gactggagca cgccgcaccc
     3781 gctgttcgtg cgcaaggcac ggggcatcga agtcgaagac gtcgacggca atacctatct
     3841 cgacttctgt ttcggtgaca cgggctcgat gttcgggcat tcgccgcccg cgattgcgcg
     3901 cgcgctcgcc gagacgggcg ccatgggcat cacctcgatg ctgcccgcgg accgcgtcgc
     3961 gcgcgtcggc gaaaaactcg cgaagatgtt cggcctgccg ttctggcaga tgacgcagac
     4021 ggcgacggat gcgaatcgcg cggcactgcg ctgggcgcgc gcgatcacgg gccgccagcg
     4081 catgctcgtg ttcgacggct gctatcacgg caccgtcgag gaaacgctgg tgcgcgcgct
     4141 gcccgacatg aacgagacgg gcggcacgcg agcgcgtgcc ggtctcatcg gcatgaacca
     4201 cgacgtgagc gtgaccaccg acgccgtgcc cttcaacgac atcgccgcgg tcgagcgcgt
     4261 gttgaagcgc acgcgcacgg cggcggtcat cgccgaaccg gtcatgacca acatcggcat
     4321 ggtgctgccg gaggaagggt tcctgcagca gttgcgcgaa gtcacgcggc gcctgcacgt
     4381 gctgctgatc atcgacgaga cgcacacgct ctccaccgcg cgcggcggtt atgcgcgcgc
     4441 gcagaagata gagcccgaca tctgggtatg cggcaaggcg atcgccggcg gcatgccctg
     4501 tgcggtattc ggcttcaccg cggaggtgga ggcgggcatg cgccgtgtgc aggaagacc
//
LOCUS       DATDEU010000568         4507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_695431, whole genome shotgun sequence.
ACCESSION   DATDEU010000568 DATDEU010000000
VERSION     DATDEU010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695431"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="VIV63_13525"
     CDS             <1..1371
                     /locus_tag="VIV63_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6125666.1"
                     /translation="NGGSAGATYGSHNTEQMFGDVAVAGDKARFGFGGSYAESEGIPT
                     FVDDDLDRGYRNVTGRASFEYSATEALTLRARGWHTNGRTEYSAQTFSDPPYAPASQD
                     FENSVYSFEGDYRRADGFGVRATVSHALDDIEQLQAGFGPAEFDYARTARTNVDVQVD
                     LASIGTHDVSLGVQRSDENTRAQSFGTVFDEDTVVTQAFVQDQFDIGRLSSRLALGGV
                     DHETFGSEVTWNAEFGAAFGSGTRVTLSGGTAFRAPDSTDRFGFGGNPDLEPEVSRQV
                     ELSVRQKLGERHQLSLSAFDNRIEDLIDYVIIDFDTFEGQNQNVERARIKGVELGYHF
                     ASEGWRARAELTLQDPRDETSDERLLRRSRESLMIAVNRDVGALDVGVDIAAVGDRKD
                     FGFPSNVTLDSYTLVNATLRYRVNGALTLQGRLENLFDEDYTYAEGYQTEGRTYTVGV
                     RYSFDD"
     gene            1387..1941
                     /locus_tag="VIV63_13530"
     CDS             1387..1941
                     /locus_tag="VIV63_13530"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY6125667.1"
                     /translation="MGNRLSKIYTRTGDDGTTGLGDGARVRKDHVRVEAYGTVDEANS
                     AIGVILSVPGLPAEVARCLTEIQHDLFDLGGELCIPGTQVIKAEQIAQLEQVLDKFND
                     PLPALKDFILPGGGPAAAHCHLARTIVRRAERCVWTLAASEQVNAEVPKYLNRLSDLL
                     FVIARVLARHEHGSEVLWRHDRGR"
     gene            complement(2030..2662)
                     /gene="ampD"
                     /locus_tag="VIV63_13535"
     CDS             complement(2030..2662)
                     /gene="ampD"
                     /locus_tag="VIV63_13535"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF008758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEY6125668.1"
                     /translation="MGRRWVPIRGRLLSHAGVARWARGAMMRVDVSTGLMVGVKQVLS
                     PYFDERPAGVVPDLIVLHGISLPPGEFGGPWVARLFTGNLPAEAHPEFRERAALRVST
                     HLLIRRDGAVEQYVSFNDRAWHAGKSEWQGREACNDYSIGIECEGTDEVPYETAQYET
                     LRVLLPMLSAAYPRITRDRIVGHSDIAPGRKTDPGPSFDWSQIKGDRSIY"
     gene            2661..4013
                     /locus_tag="VIV63_13540"
     CDS             2661..4013
                     /locus_tag="VIV63_13540"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY6125669.1"
                     /translation="MSSGNSDWMTRDLAHVWHPCTQMKDHESVPMIPLRSGKGVWLED
                     FAGSRYLDGISSWWVNLFGHSNPRINAAVREQLDHLEHAIFAGFTHPPAVLLAEELVR
                     VAPDGLNRCFFADNGSAAIEVAVKMSFHYWRNIGQQRKTRFITLSNSYHGETLGALAV
                     GNVDLYKSIYRPLLMDVITVPSPDAFLREPGMSPADHARRMFAHMETAIAENHGEVAA
                     VIVEPLVQCAGGMRMYDPVYLKLLREACDRHGVHLIADEIAVGFGRTGTMFACEQAGI
                     RPDYLCLSKGLTGGYLPLSTVLTTDAIYDAFYDEYTKLNAFLHSHSYTGNPLACAAAN
                     ATLAIFRDEPVLERNRVTAAHMLASVEHLRAHKHVAEIRQCGMILAIELMKDPVTRTP
                     WPWEQRRGLRIYRHALERGALIRPLGTTIYFMPPYVITPEEIDLLATIATEGIELACA
                     "
     gene            4004..>4507
                     /locus_tag="VIV63_13545"
     CDS             4004..>4507
                     /locus_tag="VIV63_13545"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY6125670.1"
                     /translation="MRLTRIFVEQQLVSGKGVGLPEQAGLHLTRVLRLEAGAAVTLFD
                     GTGGEFEGTLERDGKKIWARVGSHSAVERESPLQVTLIQGVARGERMDLIIQKSTELG
                     VTRIVPVFAERSVVKVDASKGARKLEHWRSIAISACEQCGRNRVPEVAEPIALADSVR
                     ALEAGTAR"
BASE COUNT          831 a         1506 c         1407 g          763 t
ORIGIN      
        1 aatggcggca gcgcgggcgc cacttacggc agccacaaca ccgagcagat gtttggtgac
       61 gtcgcagtcg ctggcgacaa agcacgcttc ggattcggcg gaagctacgc cgagagcgag
      121 ggcatcccca cgttcgtcga cgacgatctc gaccgcggat atcgcaacgt caccggccgc
      181 gcgtcgttcg aatactctgc gaccgaggcg ctgacgctgc gcgcccgcgg ctggcacacg
      241 aacggccgca ccgaatattc cgcccagacc ttttcggatc cgccgtacgc gcccgcctcg
      301 caggatttcg agaacagcgt gtactccttc gaaggcgact accggcgcgc cgacggattc
      361 ggtgttcgcg cgacggtgag ccacgcgctc gacgacatcg aacagctgca agcgggcttc
      421 ggccccgcgg aattcgacta cgcgcgcact gcccgcacga atgtggatgt gcaggtcgac
      481 ctggcttcga tcggaacgca cgacgtgagt cttggagttc agcgcagcga tgagaacacg
      541 cgtgcccaat ccttcggcac ggtgttcgac gaggacacgg tggtgacgca ggcgttcgtc
      601 caggatcagt tcgacatcgg ccggctctcg tcgcggctcg cgttgggcgg cgtggatcac
      661 gaaaccttcg gcagtgaagt cacgtggaac gccgagttcg gcgccgcgtt cggctccggg
      721 acacgcgtga cgctgtccgg cggcacggcg tttcgcgcgc ccgacagcac cgaccgcttc
      781 ggcttcggcg gcaacccgga cctcgagccg gaagtgtccc gccaggtcga attgtcggtg
      841 cgccagaagc tgggcgaacg gcaccagctg tcgctgtccg cgttcgacaa ccgcatcgaa
      901 gacctcatcg attacgtgat catcgacttc gacacgttcg aagggcagaa ccagaacgtc
      961 gagcgtgcgc gcatcaaggg cgtcgaactc ggctatcact tcgcgagcga gggctggcgc
     1021 gctcgcgccg aactcacgct gcaggatccg cgcgacgaga ccagcgatga gcgcttgctg
     1081 cggcgttcac gcgagtcgct gatgattgcc gtgaaccgcg acgtgggcgc gctcgacgtg
     1141 ggcgtggaca ttgccgcggt cggcgaccgc aaggatttcg gattcccgag caacgtgacg
     1201 ctcgattcct acacgctggt caacgccacg ctgcggtatc gcgtcaacgg cgcactgacg
     1261 ctgcagggcc gcctcgagaa cctgttcgac gaggattaca cgtacgccga gggctaccag
     1321 accgaaggcc gcacgtacac cgttggagta cgctacagct tcgacgattg aattggggag
     1381 tccgtcatgg gcaatcgctt gagcaagatc tacacacgca caggtgatga cggcaccacg
     1441 ggcctcggcg acggggcccg cgttcgcaag gatcatgtgc gcgtcgaagc ctatggcacg
     1501 gtggacgaag cgaacagcgc catcggcgtc atcctcagcg tgccgggatt gcccgcggaa
     1561 gttgcgcgct gcctcaccga aatccagcac gatctgttcg atctcggcgg cgagctctgc
     1621 atcccgggca cacaggtcat caaggccgag cagatcgcgc agctcgagca ggtgctcgac
     1681 aagttcaacg acccgttacc cgcgctcaag gatttcatcc tgccgggcgg cggccctgcc
     1741 gctgcgcact gtcacctcgc gcgaaccatc gtgcgacgcg cggagcgttg cgtatggacc
     1801 ctggccgcat ccgagcaggt caacgccgaa gtaccgaagt acctgaaccg cctctcagat
     1861 ctgctgttcg tgatcgcgcg cgtgctcgcg cgccacgaac atggttcgga agtgctgtgg
     1921 cgtcacgacc gcggaagata aaaggggaca gatctatgta ttgagaaatg gggacagatc
     1981 tatttattag ccttgtactc acgcccataa atagacctgt ccccttttct caataaatag
     2041 atctgtcccc ttttatctgg ctccaatcga acgaggggcc cgggtcggtc ttgcggcccg
     2101 gcgcgatgtc gctgtgcccc acgatgcggt cgcgcgtgat ccgcgggtag gcggctgaca
     2161 acatcggcag aaggacgcgc aacgtctcgt actgcgccgt ctcgtagggc acttcgtcgg
     2221 tgccttcgca ctcgatgccg atcgaatagt cgttgcaggc ttcgcgcccc tgccactcgg
     2281 atttcccggc atgccaggcg cggtcattga acgaaacgta ctgctcgaca gccccatcgc
     2341 ggcggatcag cagatgcgtc gacacgcgaa gcgccgcgcg ctcgcgaaat tccgggtgcg
     2401 cttcggccgg caggttgccg gtgaacagac gcgcgaccca cggcccaccg aactcacccg
     2461 ggggcagact gattccatgc aggacgatca gatcgggaac gactccggcc ggccgctcgt
     2521 caaaatatgg tgacaagacc tgtttgactc cgaccatcag gccggtactg acatcgacgc
     2581 gcatcatggc tcctcgcgcc catcttgcca cacccgcgtg cgacaatagc cgccctcgaa
     2641 tcggcaccca tctgcgaccc atgagctccg gcaattccga ctggatgacg cgcgatctcg
     2701 cgcacgtgtg gcatccctgc acccagatga aggatcacga gtcggtgccg atgattccgc
     2761 ttcgctccgg gaaaggcgtg tggctcgaag acttcgcggg ctcgcgttac ctcgatggca
     2821 tcagctcctg gtgggtcaac ctgttcggcc attcgaaccc gcgcatcaac gccgcggtgc
     2881 gcgagcagtt agaccacctc gaacacgcca tcttcgccgg cttcacgcat ccgcccgcgg
     2941 tgctgctcgc cgaagaactg gtgcgcgtgg cgcccgacgg cctgaaccgc tgcttcttcg
     3001 ccgacaacgg ctcggccgcc atcgaagtcg ccgtgaagat gagcttccac tactggcgaa
     3061 acatcggcca gcagcgcaag acgcggttca tcacgctgtc gaacagctat cacggcgaga
     3121 cgctcggtgc gctcgcggtg ggcaacgtgg acctctacaa gtccatctac cgcccgctgc
     3181 tcatggacgt gatcaccgtg ccctcccccg atgcgttcct gcgcgagccc gggatgagcc
     3241 ccgccgacca tgcgcgccgc atgttcgcgc acatggaaac cgccatcgcc gagaatcacg
     3301 gcgaggtcgc cgcggtcatc gtcgagccgc tggtgcaatg cgccggcggc atgcgcatgt
     3361 acgaccccgt ttacctcaaa ctactgcgcg aagcctgcga ccggcacggc gtacacctga
     3421 tcgccgacga gattgccgtg ggcttcgggc gcacgggcac gatgttcgcg tgtgaacagg
     3481 cgggcatccg gccggactat ctatgcctgt cgaagggcct gacgggcggc tacctgccct
     3541 tgtccacggt gctgaccacc gatgccatct acgacgcctt ctatgacgag tacacgaagc
     3601 tcaacgcatt cctgcactcg cacagctaca ccggcaatcc gctggcctgc gccgcggcca
     3661 atgccactct cgccattttc cgcgatgagc ccgtgctcga acgaaatcgc gtgaccgcgg
     3721 cccacatgct cgccagcgtc gaacacctgc gcgctcacaa gcatgtcgcc gagatccgtc
     3781 agtgcggcat gatcctggcc atcgaactca tgaaggatcc cgtcacccgt acgccgtggc
     3841 cgtgggagca gagacgcggg ctgcgcatct accggcacgc gctcgaacgc ggcgcgctca
     3901 tccgcccgct cggcactacc atctacttca tgccgcccta cgtcatcacg cccgaagaaa
     3961 tcgatctgct ggcgacaatc gccaccgaag gcatcgagct cgcatgcgcc tgacccgcat
     4021 cttcgtcgag cagcagctcg tctccggcaa gggtgtcggg cttcccgaac aggcaggcct
     4081 gcatctcacc cgagtgctac ggctcgaggc cggtgcggcc gtgacgctgt tcgacggcac
     4141 cggcggcgag ttcgagggca cgcttgagcg cgacggcaag aagatctggg cgcgggtggg
     4201 ctcgcattcc gccgtcgagc gcgaatcgcc gctgcaggtg acgctgatcc agggcgtcgc
     4261 gcgcggcgag cgcatggacc tcatcatcca gaagtcgacc gagcttggcg tgactcgcat
     4321 cgtgcccgta ttcgcggagc gctccgtggt gaaagtggat gcaagcaagg gtgcgcgcaa
     4381 gctcgagcac tggcgttcga tcgcgatctc cgcgtgcgag caatgcgggc gcaatcgcgt
     4441 gcccgaggtc gcggagccga tcgcgctggc agactcggtg cgggcactcg aggccggcac
     4501 cgcgcgt
//
LOCUS       DATDEU010000569         9498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1571048, whole genome shotgun sequence.
ACCESSION   DATDEU010000569 DATDEU010000000
VERSION     DATDEU010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571048"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="VIV63_13550"
     CDS             complement(<1..2004)
                     /locus_tag="VIV63_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY6125671.1"
                     /translation="MAIRNVFGAQSIRQKLLLGTLFLAIIPVAVTSIIVGRESLNSGK
                     AALESQARESLIAQRASKAAQITDYFDSLSNQVQVLAASPDVINAMREFPAAYENSVL
                     NVADLPAERARLAKYYTSEYMAEFQRRNTGKMVNMADSAAALPDLAMNLQYQYIAGNP
                     HPLGSKFNLDRANDGSRYSELHGALHPYLRTALRQFGLYDIFLIEPRNGTIVYTVFKE
                     LDFATSLVSGPFAKSKLGDAFRASWGLDKPGMVALSEFGEYLPSYNDQAAFLGTPIFE
                     GGKKIGVLVVQVPIDKINSVMTHEGKWKERGLGNTGETYLVSAADGTPRSVARLAVED
                     INAYAKSVSDAGFAQGLANAVQAKGTSIGLVPIKTQATQDVFEKGSSGFGVYPNYAKR
                     PVLGSYAPLSVLGLKWAIIAEENATEAFAPVTAVREKVVVWSTGIAVALLGLGLLIAF
                     SLLRSVTRPITKLQATVAAIADGDYAARSQLGGDDEFGQLSRAFDDLLDDRVARLADA
                     ERENEDLNNSVVQLLQALAQLSNKDLTAKAPVTANVIGTVSDSINLLSYETGRVLSEV
                     TSIAAQVKLSTERVKRQGESVTATAAAERSDVERAGENLANASKVMTQVAELAAASNR
                     AAEQATSSTVTALASVNETVKGMEGIRESIAESEKRIKRLGERS"
     gene            complement(2009..2521)
                     /locus_tag="VIV63_13555"
     CDS             complement(2009..2521)
                     /locus_tag="VIV63_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY6125672.1"
                     /translation="MNATFPLPVGLPPLSAALAALAAGPVEENPNDIVRQAFRIGHMR
                     LLAPFATASELVEMPNVYPLPRMPANLLGLVNLHGRIVPVFDLAPTFETQHLPREKRM
                     LLVIGHGDAAAGIVIDGLPRRLVFQPENEIITPALSPAASAAVVATYANGQDAWFEFN
                     YAQLLDQLVS"
     gene            complement(2542..2907)
                     /locus_tag="VIV63_13560"
     CDS             complement(2542..2907)
                     /locus_tag="VIV63_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6125673.1"
                     /translation="MAIHKVLIVDDARPDLMNLEKIVSGAGYVTLTAINGQQAVEMSK
                     SQMPDVILMDVNMDTMDGFAATRAIGGDAATKTIPVVLVTSKSQKADRVWAQMLGVKG
                     YVTKPYTPDQILSAISACA"
     gene            complement(3120..5039)
                     /locus_tag="VIV63_13565"
     CDS             complement(3120..5039)
                     /locus_tag="VIV63_13565"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY6125674.1"
                     /translation="MSPHSRRDALKLLGASAAVAAAPGARGGAVEIEVLEPKFFPLRD
                     VRLGTGPFLDAQKLDEAYLLRLEPDRMLHNFRVNAGLTPKAPVYGGWESVEPWIWIRC
                     HGHTLGHYLTAAACMFESTGDARFAERVDYIVAELAECQSKTAGWLSAFPDGIEPLTN
                     SLAGKEFPGVPWYTVHKVLAGLRDAHLHRGSAQAFDIWLKFTDWIDSATRDVPVADFQ
                     KMLDREHGGMNEVCADLFAITKTPAHLALAHRFCHLALLNPLADGKDALDGLHANTQI
                     PKVIGFDRIDAVSGNPRLHQAALFFWNSVVTQRSYATGGHGDLEHFFPKTEFAQRLGS
                     GKTMETCCTHNMMRLTRSLCSKGMFTRHIDYFERALYNGILASQDPQSGMMSYFQSTR
                     PGYVRLYHTPFDSFWCCTGSGMENHARYGETIFAHQQNNLVVNLFISATLDWRELGLT
                     VTQQTRFPDADTTRLMFSAKKPRTINLHLRQPSWCPVMTIHVNGKLKATARLPGFGYS
                     IERKVRSGDVVEVRLPMSLRVETLPNAPEYAALMYGPIALGGRFGTDGLTPGSQLIVN
                     ERESGNMLQADVKIPRWTRPLEDLVANTTRTNPEKLEFRTSGFEGGAAVDLIPWFRLT
                     NERYNLYWRREPIPA"
     gene            complement(5042..6685)
                     /locus_tag="VIV63_13570"
     CDS             complement(5042..6685)
                     /locus_tag="VIV63_13570"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6125675.1"
                     /translation="MKQHSSRRRFLSQMGLAGAGIAATSVFSLPSYSQGSGRTARATL
                     GLIAPRADFDRRLFGAFLEHLGRAVYTGVYEPGSKLADKRGFRTDTLREVRDLNVPIM
                     RYPGGNMVSGYNWWDGVGPKKDRPTVLERAWNQIETNQFGTNDFMDWAKAVGTEPLLG
                     FNLGTGTSEMAVAYIEYCNYAKGTKWSELRRSHGYDAPHKVKYWCLGNEMDGPWQMGR
                     LTAREYGRKARDIAQQARVIDPDVQLIACGSSNTIMSTYLTWDREVLEECYDMVDGIS
                     LHNYFGNEPELAGGKSERFLAQNLDMERQIQEITAVADYVQGLYRSPKKLWLSFDEWN
                     VWYRARSTEHLDGKGAYAPKLLEEVYNLEDALLTGGFLNTLMRQSDRVRLACLAQIVN
                     VIAPLVTNEKGILRQSIFFPYAMVLKYAHGRMLDIRVESETYPIRAAGLRPDFARNEE
                     VPFLDVVATYDAKQKQIAVFALNRDLSNERELALTFEDVAPSKVLACETITGPDLKAF
                     NTFESPNQVVTTKLDVASAGAKMSVKLPPRSYTVVHLAV"
     gene            complement(6960..7970)
                     /locus_tag="VIV63_13575"
     CDS             complement(6960..7970)
                     /locus_tag="VIV63_13575"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY6125676.1"
                     /translation="MKRPFGTLSDGRAVEAITLGTEQGLKLEVLTYGAILHRLTYPVR
                     GVRRDLILHFDDVQQYERDRAYVGSLVGRYGNRIGGSRFELDGKVHEVTANEGVNHLH
                     GGALGFGKRLWRVLDITDHETQRVLLALDSPDGEEGYPGNVEVTAELTVKSDALSIIL
                     SARSDAATPINLTYHPYFNLQADARDPAIEQRLRIPASHYLPVAAGLIPTGELASVEG
                     TTFDFRASRRLRPPPADSHPQLALAGGYDHCWVLAEDADCACELSSPNGDMTLTMLGG
                     PGLQFYNGQFLARSHPSIGSGVILEPQGFPNAPNEPRFPKAVLRAGELYRVGIEYRVA
                     AR"
     gene            complement(7967..8956)
                     /gene="yjfF"
                     /locus_tag="VIV63_13580"
     CDS             complement(7967..8956)
                     /gene="yjfF"
                     /locus_tag="VIV63_13580"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF008630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HEY6125677.1"
                     /translation="MSALALPAFDRRLVPLSATLVLFLAMVAFGALAYDGFLAPQVFL
                     NLAIDNAFLLIAAIGMTFVILTGGIDLSVGSVLALTTMAAAALIEHAQWSPLLVIPLL
                     LAGGAVFGALQGFLIHRYRLQPFIVTLAGMFVARGLCYLIDTESIPVRDPLFVTLAEM
                     RIPLGDSSLSAGAAIALVTVLLALWLAHATRFGRTVYAIGGNARSAALMGLPVGGTLV
                     GVYALSGFCAALAGITMTVYMLSGYSLHAVGLELDAIAAVVIGGTLLTGGVGYVAGTV
                     VGVLILGIIQTLVIFDGTLSSWWTRIVVGILLFLFCVAQRLIEGRAGKNGQRA"
     gene            complement(8953..>9498)
                     /locus_tag="VIV63_13585"
     CDS             complement(8953..>9498)
                     /locus_tag="VIV63_13585"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125678.1"
                     /translation="YDFIGNGFLFGLPFAAVLAVAVIVALHVLLSATPLGLFIRAIGI
                     NPLAARIAGVRARPITLALYSFCGLTAGLAGLIISSNVKSADANNAGTLLELDAILAV
                     TLGGTALTGGRFSLAGTAIGAMIIQTLTSTIYSLGVPPEVNLVFKAALIFVVMLLQSA
                     EFRASIRRIAVRPAQPEGPAT"
BASE COUNT         1671 a         2902 c         3254 g         1671 t
ORIGIN      
        1 cgaacgttcg cccagacgct tgatgcgttt ttcggattcg gcgatggatt cgcgaatgcc
       61 ttccatgcct ttcacggtct cgttcaccga cgcgagcgcg gtgaccgtgg aggaggtcgc
      121 ctgctcggcg gcgcggttgg aagcggccgc gagctcggcc acctgggtca tgaccttgga
      181 cgcgttcgcc aggttctcac ccgcgcgttc gacgtccgag cgttcggccg cggcggtcgc
      241 cgtcaccgat tcaccctggc gcttcacgcg ttcggtggag agcttcacct gcgcggcgat
      301 gctggtcact tcggacaaca cgcgtccggt ctcgtatgac agcaggttga tggagtcgga
      361 cacggtgccg atcacgttcg cggtgaccgg cgccttggcg gtcaggtcct tgttcgagag
      421 ctgcgccaag gcctgcagca gctgcacgac cgagttgttg aggtcttcgt tctcgcgctc
      481 ggcgtcggcg aggcgcgcca cgcggtcgtc gagcaggtcg tcgaaggcgc gggagagctg
      541 gccgaattcg tcgtcgccgc cgagctgcga acgcgcggcg tagtcgccgt cggcgatcgc
      601 ggccacggtc gcctgcagct tggtgatagg acgcgtcacg ctgcgcaaca gcgagaaggc
      661 gatgagcagg cccaggccga gaagcgcgac ggcgataccg gtcgaccaca ccacgacttt
      721 ctcgcgcacc gcggtcacgg gcgcgaacgc ttccgtggcg ttttcttcgg cgatgatcgc
      781 ccacttgagg cccagcaccg acagcggcgc ataggaaccg agcacggggc gcttggcgta
      841 gttgggatac acgccgaagc cggaggagcc tttctcgaat acgtcctgcg tcgcctgcgt
      901 cttgatgggg acgagtccga tgctcgtgcc cttggcctgc acggcattgg cgaggccctg
      961 cgcaaagccc gcgtcgctca cgctcttggc gtaggcattg atgtcttcca cggcgagacg
     1021 cgcgaccgaa cgaggggtgc cgtcggcggc ggaaacgagg taggtctcgc cggtgttacc
     1081 gagaccgcgc tctttccact tgccttcgtg cgtcatcacc gagttgatct tgtcgatggg
     1141 cacctgcacg acgagtacgc cgatcttctt gccgccttcg aaaatcggcg tgccgaggaa
     1201 cgcggcctgg tcgttataag agggcaggta ttcaccgaac tccgacagcg cgaccatgcc
     1261 gggcttgtcg aggccccagg acgcgcggaa ggcgtcgccg agtttcgact tggcgaaagg
     1321 tccgctcacc agcgaggtgg cgaaatcgag ctccttgaac accgtgtaga cgatggtgcc
     1381 gttgcgcggc tcgatcagga agatgtcgta gaggccgaac tgccgcagcg cggtgcgcag
     1441 gtacggatgg agggcgccgt gcagttcgct gtagcgcgag ccgtcgttgg cgcggtcgag
     1501 gttgaacttc gagccgagcg gatgcgggtt cccggcgatg tactgatact gcaggttcat
     1561 cgcgaggtcg ggcagcgcgg ccgcgctgtc ggccatgttg accatcttgc cggtgttgcg
     1621 gcgctggaac tcggccatgt attcgctggt gtagtacttc gcgaggcgag cacgttcggc
     1681 gggaaggtcg gcgacgttga gcaccgaatt ctcataggcg gccggaaatt cgcgcatcgc
     1741 gttgatgacg tccggcgagg cggccagcac ctgcacctgg ttggagagcg agtcgaaata
     1801 gtcggtgatc tgcgcggcct tcgaggcgcg ttgggcaatc aacgattcac gcgcctggga
     1861 ttcgagcgcg gccttgccgg agttgagcga ttcgcgtccg acgatgatcg acgtgaccgc
     1921 caccgggatg atggcgagga acagcgtacc gagcagaagt ttctgccgga tggactgggc
     1981 gccgaatacg ttgcgaatgg ccatggtgtc agctcacgag ttgatcgagt agttgcgcgt
     2041 agttgaattc gaaccaggcg tcctgcccgt ttgcataggt cgcgacgacc gcggccgaag
     2101 cggccggcga taacgccggc gtgatgattt cgttttcggg ctggaacacc aggcggcgcg
     2161 gaaggccgtc gatgacgatg cccgcggccg cgtcaccgtg gccgatcacc agcaacatgc
     2221 gtttctcgcg cggcaggtgt tgcgtctcga acgtgggagc gagatcgaat accggcacga
     2281 tgcggccatg caggttcacg aggcccagca ggttggcggg catgcgcggc agtggataga
     2341 cgttgggcat ctcgaccagt tcgctggcgg tcgcgaacgg cgcgagcagg cgcatgtgcc
     2401 cgatgcggaa cgcctggcga acgatatcgt tcgggttttc ttccaccggt cccgcggcca
     2461 acgccgccag cgccgcggag agcggaggca ggccaacggg cagtggaaag gtggcgttca
     2521 tgatttcggt ggcgttgctc tttaagcgca ggcggagatg gcgctgagaa tctggtccgg
     2581 cgtatacggc ttcgtgacgt agcccttcac gcctaacatc tgggcccaca cgcggtcggc
     2641 cttctggctc ttgctggtga cgagcaccac ggggatggtc ttggtggcgg catcgccacc
     2701 gatggcgcgc gtggccgcga agccgtccat cgtgtccatg ttgacgtcca tgagaatgac
     2761 gtcgggcatc tgcgacttcg acatctccac ggcctgctgg ccgttgattg ccgtgagagt
     2821 gacgtagccg gcgccgctca cgatcttctc gagattcatg agatcaggac gcgcgtcatc
     2881 gacgatcagc actttatgaa tagccactgc ttgctccctc acgcgcaatg aggccccgcc
     2941 gaccctttcg gacggtttct cccattggca cgaggggaat cttggcggga atgtgacaaa
     3001 accacggtga cgcgggtcac acggggtgtc cctaggaaag gctccagttc gcgcttttcc
     3061 ggggtttcga gcagcggccg ggtgaacggt gtcatccggc cgaccagcgg tgccgggcct
     3121 taggcgggaa tcggctcccg gcgccaatag aggttgtagc gctcgttcgt gagccggaac
     3181 cagggaatga ggtccacggc ggctccccct tcgaaccccg acgtgcggaa ttcgagtttc
     3241 tcgggattcg tgcgtgtggt gttcgccacc aggtcctcga gcggccgcgt ccagcgcggg
     3301 atcttcacgt cggcctgcag catgttgccg gactcgcgct cattgacgat gagttgagag
     3361 ccgggagtca ggccatcggt gccgaagcgt ccgccgagcg caatggggcc gtacatcagc
     3421 gcggcgtact ccggagcgtt cggcagagtc tcgacgcgca gtgacatggg caggcgcact
     3481 tcgacgacgt cgcccgatcg gaccttccgt tcgatcgagt agccgaagcc gggcaagcgc
     3541 gcggtggcct tcagtttgcc gttcacgtgg atggtcatga ccggacacca cgagggctgg
     3601 cgcaggtgca ggttgatcgt gcgcggcttc tttgcgctga acatcagacg tgtggtgtcg
     3661 gcatccggga agcgggtctg ctgcgtcacc gtgagcccga gttcgcgcca atcgagcgtc
     3721 gcgctgatga acaggttcac cacgaggttg ttttgctggt gagcgaaaat cgtttcgccg
     3781 tagcgcgcgt ggttttccat tcccgatccg gtgcagcacc agaacgaatc gaacggcgtg
     3841 tgatagagcc gcacgtaccc cggccgcgtg gactggaagt aactcatcat gccgctctgc
     3901 gggtcctgcg aggcgaggat gccgttgtag agcgcgcgtt cgaaatagtc gatgtgccgg
     3961 gtaaacattc ccttggagca cagcgaacgc gtgagacgca tcatgttgtg cgtgcagcag
     4021 gtctccatgg ttttgccgga gccgaggcgc tgcgcgaact ccgtcttggg gaagaagtgc
     4081 tccagatcgc cgtgcccacc cgtggcgtag gaacgctggg tcaccaccga attccagaag
     4141 aaaagggcgg cctggtgaag acgcgggttg ccgctcacgg catcgatgcg atcgaaaccg
     4201 atgaccttgg ggatctgcgt gttcgcgtga aggccgtcga gcgcatcctt gccgtcggcg
     4261 aggggattga gcagcgccag gtggcagaag cgatgcgcca gcgcgagatg tgcgggagtc
     4321 ttcgtgattg cgaagaggtc ggcgcagacc tcgttcatgc cgccgtgttc gcggtcgagc
     4381 atcttctgga aatcggccac cggcacatcg cgagtggccg aatctatcca gtcggtgaat
     4441 ttcagccaga tgtcgaaggc ctgcgcgctg ccgcgatgca ggtgcgcatc gcgcaggccg
     4501 gcgagcacct tgtgcacggt ataccagggc acgcccggaa actccttgcc ggcgaggctg
     4561 ttcgtgagcg gttcgatgcc atcgggaaag gccgacaacc aacccgccgt cttcgactgg
     4621 cactcggcca gttccgcgac gatgtaatcg acgcgctccg cgaagcgcgc gtcacccgtc
     4681 gattcgaaca tgcaggccgc ggccgtgagg taatgaccga gcgtgtggcc atgacaccgg
     4741 atccagatcc agggctccac cgactcccag ccgccataca ccggggcctt cggcgtgagc
     4801 cccgcattga cccggaagtt gtgcagcatg cgatcgggct cgagccgaag tagataggct
     4861 tcgtcgagct tctgcgcgtc gaggaagggc ccggtgccca ggcgtacgtc gcgcagcggg
     4921 aagaatttcg gctcaagcac ctcgatttcc acggcgccgc ctcgggcgcc gggagccgcc
     4981 gccaccgccg cgcttgcgcc cagcagcttc aatgcatcgc ggcgcgagtg gggcgacatg
     5041 gtcagaccgc caggtggacg acggtgtacg aacgaggcgg cagcttcacg gacatcttcg
     5101 cgccggccga agccacgtcg agcttcgtgg tcaccacctg gttcgggctc tcgaaggtgt
     5161 tgaaggcctt gagatcgggt cccgtgatcg tttcgcaggc gagtaccttc gaaggagcaa
     5221 cgtcctcgaa cgtgagcgcc agctcgcgct cgttcgacag gtcgcgattg agcgcgaaca
     5281 cggcgatctg cttctgcttc gcgtcgtagg tcgcgacgac gtcgaggaac ggcacctctt
     5341 cattacgcgc aaagtcgggg cgcaagcccg cggcacggat cggataggtc tcgctctcga
     5401 ctcggatgtc gagcatgcgg ccgtgtgcgt acttgagcac catggcgtag gggaagaaga
     5461 tgctctggcg caggatgccc ttctcgttgg tgaccagcgg cgcgatcacg ttgacgatct
     5521 gcgcgaggca ggcgaggcgc acgcgatcgg actggcgcat cagcgtgttg aggaagccgc
     5581 ccgtgagcaa cgcgtcttcg aggttgtaga cttcttcgag cagcttcggt gcgtacgcgc
     5641 ccttgccgtc gagatgttcg gtgctgcggg cgcgatacca cacgttccat tcgtcgaacg
     5701 acaaccagag cttcttgggc gagcggtaca ggccctgcac gtagtcggcg accgcggtga
     5761 tctcctggat ctgccgctcc atgtcgaggt tctgcgcgag gaaacgttcg ctcttgccgc
     5821 cggcgagctc gggctcgttg ccgaagtagt tgtgcagcga tatgccgtcg accatgtcgt
     5881 agcactcttc gagcacttcg cgatcccacg tgaggtacgt ggacatgatc gtgttgctcg
     5941 aaccgcaggc gatgagctgc acgtcggggt cgatgacccg cgcctgctgg gcgatgtcgc
     6001 gcgccttgcg cccgtactcg cgcgccgtga gtcggcccat ctgccacgga ccgtccattt
     6061 cgttgccgag gcaccagtac ttcaccttgt gtggcgcatc gtagccgtgc gaccggcgca
     6121 gctcgctcca cttcgtgccc ttcgcgtagt tgcagtactc gatgtacgcg accgccatct
     6181 cggaagtgcc cgtgcccagg ttgaagccca gcaggggttc ggtacccacg gccttcgccc
     6241 agtccatgaa gtcgttggtg ccgaactggt tggtttcgat ctggttccac gcgcgttcga
     6301 gcaccgtggg acggtctttc ttcggaccta cgccatccca ccagttgtaa ccggaaacca
     6361 tgttgccgcc cgggtaacgc atgatcggga cgttgaggtc gcgcacttcg cgcagcgtgt
     6421 ccgtgcggaa gccgcgcttg tcggccagct tcgagccggg ttcataaacg ccggtataga
     6481 ccgcgcggcc caggtgctcg agaaacgcgc cgaacaggcg gcggtcgaaa tcggcgcgtg
     6541 gcgcaatcag gccgagcgtt gcgcgcgcgg tacgtccgct gccctgcgag tagctaggca
     6601 gcgagaaaac ggaagtggcg gcgatgccgg ccccggcgag acccatctgt gaaaggaagc
     6661 ggcgtcggct cgagtgctgt ttcattgttg tactccctca tagacaaaac gcgcgccagt
     6721 atagtcagcg cgccgttgca gttcgggcat cggtcgatac cggatttcgc atcgcggaag
     6781 gcgacgaaag ccggacgatt cgaagtttat tttttgaggt accgttacca cacatggacg
     6841 atgcaccaac cggtgcatac gatccctctc gcgtcttcgc atgcccgcac gcatgcagcc
     6901 gtggaacgaa cccggcgccg ccttccgcaa aagttgcgcg gcggcgccgg acccggaact
     6961 caccgggccg ccacgcgata ttcaataccc acccgataca actctccggc acgcagcacg
     7021 gctttcggaa agcgcggctc gttcggcgca ttcggaaatc cctgcggttc caggatcacg
     7081 ccactgccga tcgacggatg gctgcgcgcc aggaactgcc cgttgtagaa ctgcagcccc
     7141 ggcccgccca gcatcgtgag cgtcatgtcg ccgttcggcg aggagagctc gcaggcgcaa
     7201 tcggcatcct ctgcgagtac ccagcaatgg tcgtatccgc cggcgagcgc gagttgcgga
     7261 tgcgaatcgg cgggcggagg ccgcagccgg cggctcgcac gaaaatcgaa cgtggttccc
     7321 tcgacggatg cgagctcacc cgtggggatg aggccggcgg cgaccggtag atagtggctg
     7381 gcggggatgc gcaggcgttg ttcgatggcc ggatcgcggg catcggcctg caggttgaag
     7441 tacgggtggt aggtcagatt gatgggcgtg gcggcatcgc tgcgcgcgct caggatgatg
     7501 gacagcgcat cggacttcac ggtgagctcg gcggttacct cgacattgcc cggataccct
     7561 tcctcgccgt cgggcgaatc cagggcgagc agcacgcgtt gggtttcgtg gtcggtgata
     7621 tccagcacgc gccagaggcg tttgccgaag cccagggcgc cgccatgcag gtgattcacg
     7681 ccttcgttgg ccgtcacctc atggaccttg ccatcgagtt cgaatcgcga gccaccgatg
     7741 cggttgccgt accggccgac cagcgatccg acgtacgcgc gatcgcgctc gtactgctgc
     7801 acgtcgtcga agtggaggat gaggtcgcgg cgcacgccgc gaacgggata ggtgagccgg
     7861 tgcaggatgg cgccgtaggt gaggacttcg agtttcagcc cttgctccgt gccgagcgtg
     7921 atggcctcga cggcgcggcc atccgacaat gtgccgaaag gacgtttcat gcgcgttgcc
     7981 cgttcttgcc ggcgcggcct tcgatgagcc gctgcgccac gcagaaaaga aacagcagga
     8041 tgccgaccac gatgcgcgtc caccacgaac tcaaggtgcc gtcgaagatc accagcgtct
     8101 ggatgatgcc gaggatgagc acgcccacca ccgtaccggc gacgtaaccc acgccgccgg
     8161 tgagcagggt accgccgatg accaccgcgg cgatggcatc gagctcgagg ccgacagcgt
     8221 gcaggctgta accggagagc atgtacaccg tcatcgtgat gccggcgagc gccgcacaga
     8281 agccgctgag cgcgtacacg cccaccagcg ttccgcccac gggcagtccc atgagcgccg
     8341 ccgagcgggc gttaccgccg atggcgtaca ccgtgcggcc gaatcgcgtc gcgtgcgcca
     8401 gccacagcgc cagcaagacg gtcacgagcg cgatcgcggc gccggcggac aacgagctgt
     8461 cgccgagcgg aatgcgcatc tccgcgagcg tgacgaacag cggatcgcgc acgggaatgg
     8521 attcggtgtc gatgagataa caaagcccgc gtgccacgaa catgccggcc agcgtgacga
     8581 tgaagggttg caggcggtat cggtggatca ggaatccctg caacgcgccg aaaaccgcgc
     8641 cgcccgccag cagcagcgga atgaccagca gcggactcca ctgggcgtgt tcgatcagcg
     8701 cggcggcggc catagtcgtg agcgccagca cggagcccac cgacagatcg atgccgcccg
     8761 tcaggatcac gaaggtcatg ccgattgcgg cgatgagcag gaacgcgttg tcgatcgcga
     8821 gattgaggaa cacctgcggc gcgaggaacc cgtcgtaggc cagcgcgccg aaggccacca
     8881 tggccaggaa cagcaccagc gtggccgaca gcggcacgag gcggcgatcg aacgcgggca
     8941 gggcgagggc gctcatgtcg caggtccttc cggctgggca ggccgcaccg cgatcctgcg
     9001 aatgctggcg cggaactccg ccgactgcag cagcatcacc acgaagatca gcgccgcctt
     9061 gaacaccagg ttgacctcgg gcggcacgcc gagcgagtag atagtggaag tgagcgtctg
     9121 gatgatcatc gcgccgatgg ccgtgcccgc gagactgaag cggccgccgg taagagcggt
     9181 gccgccgagc gtcacggcga ggatggcgtc gagttcgagg agcgtacccg cgttgttggc
     9241 gtccgcgctc ttgacgttgg agctgatgat gagcccggcg agtccggcgg tgagcccgca
     9301 gaagctgtac agcgcgagcg tgatgggccg ggcgcgcacg ccggcgatcc gggcggccag
     9361 cgggttgatg ccgatggcgc ggatgaagag ccccagcggc gtggccgaga gcaatacatg
     9421 cagcgccacg atgacggcca cggcgagcac ggccgcgaaa ggcagcccga acaggaagcc
     9481 gttgccgatg aagtcgta
//
LOCUS       DATDEU010000570         7442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_987798, whole genome shotgun sequence.
ACCESSION   DATDEU010000570 DATDEU010000000
VERSION     DATDEU010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987798"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            560..1372
                     /locus_tag="VIV63_13590"
     CDS             560..1372
                     /locus_tag="VIV63_13590"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY6125679.1"
                     /translation="MAVNKKNRKDSRKGRKQETAGAHPHRKPLRLHGTIARDLGVLIV
                     SGRYRAGHVLDGEVEASEQRKVSRTAYREALRILAAKGLVHSRPRVGTRVSPLENWHL
                     LDPDVLSWAFSGEPEPEVLHGLFELRTIVEPAAAALAASRRNQKHLDSMRRALDAMTV
                     HTLNVEEGRIADKEFHAALLLATANPFIVSLTNGVTAAVNALTEFKQRIAPLKRDPVP
                     DHWRVYDAIAAKDAEGARAAMTELIRLAIMDTPTKQRPKHAVASRDSRAAHT"
     gene            1629..3200
                     /locus_tag="VIV63_13595"
     CDS             1629..3200
                     /locus_tag="VIV63_13595"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6125680.1"
                     /translation="MTRSRTKWLVAAVFGTCVMIAGSATARAAEAVNVSVTIRADQPG
                     EKINPNVYGQFAEHLGAGIYEGVWVGEKSTIPNTHGYRNDVIEALRNLKVPVVRWPGG
                     CFADEYHWRDGIGDRAKRPVKVNTHWGGVAETNEFGTHEFMAFAEMIGTKVYISGNVG
                     SGSPQEMADWMEYMTSNTVSTLANERRKNGREQPWDGHYFGIGNETWGCGGNMRPEYY
                     ADLFRQYATYVKAPRGKRPVIVASGGNDEHTIWAEVLTKMVERDMDAISHHYYTIPGK
                     EWRQKGKSIGFTEAEWISTLNHTMRIDEYITNNVKILDQNDPKGKVAFYVDEWGTWYD
                     PEPGREPGFLWQHNTLRDALVAGLNFNIFHRHAKRVQMTNIAQMVNVLQAMILTEGPK
                     MALTPTYHVFQMYIPFQGATYLPTEITTPEYKLADYTVPSVSVSAARDTDGRLQLALV
                     NLDPHREAVVTTSLTGAKASAALGRVLTSKTMDARNTVDAPEAITPVKISATRKGDAL
                     VLRLPPKSVSVVRLQ"
     gene            complement(3162..3416)
                     /locus_tag="VIV63_13600"
     CDS             complement(3162..3416)
                     /locus_tag="VIV63_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125681.1"
                     /translation="MTIKRKHLARIGTVAAAFLPAFALAHPGHGDTSTFFGEVMHQLS
                     WQHALGFLFSGAALIAAGVAAIRLWSSRLLKPNHTHRLRR"
     gene            3511..4128
                     /gene="ureG"
                     /locus_tag="VIV63_13605"
     CDS             3511..4128
                     /gene="ureG"
                     /locus_tag="VIV63_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEY6125682.1"
                     /translation="MSTKSDVLRVGVGGPVGSGKTALVDSLCKRMRDDFNIAVVTNDI
                     YTKEDQEFLIRSQALAPERIRGVETGGCPHTAIREDASMNLAAIDQLMHDFTGLDFII
                     VESGGDNLAATFSPELSDLTIYVIDVAAGDKIPRKGGPGIMKSDLLIINKIDLAPHVG
                     ASLEVMDRDAKKMRGERPFVFTNLKTGQGLDQVVQFIEKQGMLRH"
     gene            4218..4718
                     /locus_tag="VIV63_13610"
     CDS             4218..4718
                     /locus_tag="VIV63_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125683.1"
                     /translation="MQGQPFRVLFLSQRNSARSMMAEAIANTIGHGQLQAFSAGVRPA
                     SRIDPIAIELLEHAGVQAPSHTPQHVREFSAPESPPLDFVFTLSDTAAGEGPPSWPGH
                     PITAHWRCEDPEQFDDPVEKRQSLIRTRAELERRLRVLTNLPVRSLDRLSLQTQVDSL
                     GRTGAS"
     gene            4782..5801
                     /locus_tag="VIV63_13615"
     CDS             4782..5801
                     /locus_tag="VIV63_13615"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY6125684.1"
                     /translation="MRTNILSICVLLGVAPVLAQSPESELPLDEVVVTGEYPGPGMWK
                     VTRADDARGHVMWIVGDPPPLPKRMKWKSKDVEAVALGAQEILRDSSVSMEPDEKIGF
                     FRGMTLLPAMLEARRNPDDAKLKDLVPPDLYARWLVQKKLYLGRESGVESWRPLFAAD
                     KLRKAAYDDLKLRESGAVWEVIGKLAEKHKIKVTSPTLRFTIKRSEVRTKIKEFSRES
                     LPDVECFETMLRLTEALGDRETQNARAHAWATGDVEGLTRLPVLPSPYLPCAMAVMNS
                     QVAREVIPADIREKVHALWLEAAEKSLAANTTTFAIVPFVKLTRADGYLAKLRAKGYL
                     IEAPQ"
     gene            complement(5811..6440)
                     /locus_tag="VIV63_13620"
     CDS             complement(5811..6440)
                     /locus_tag="VIV63_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HEY6125685.1"
                     /translation="MSCALSAGCTASRGPEELTDDGLVRVPSRRVAGVYRAPGATFTQ
                     YQRIMLEPPTIEFVRDWRKNHSDVNDTEYARIVRESAELFREEFTREIIDRGPYEYAD
                     ELAPDVLIVNPAIQDLDIVAPDASTAPGERQYTTGPVKMKVTGELRDAATGKLVGRLT
                     IYEGNERYAFNELRLANRVTNAHEQRTAFAKWARILNEALAVAKVERPR"
     gene            complement(6522..>7442)
                     /locus_tag="VIV63_13625"
     CDS             complement(6522..>7442)
                     /locus_tag="VIV63_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6125686.1"
                     /translation="TTSHQSDVRRDTILVVDDTPETLGFLTDTLDHAGFTALIATDGE
                     SALRLIEQITPDLVLMDAVMPGLNGFETCRRMKRNKMLAHLPVIFMTGLTETENVLEG
                     LAAGGVDYVTKPLVVDELLARIRVHLANARVAAGTRAALDATGRFLLATDRAGRLLWC
                     TPKARQLLAELFPAASESNASLPSAMMGQLMQLRSEGGNTRLLVDINGRKVEISVLSP
                     IGPDELLFRLTELSTTADEQQLQQTLSLTSRESEVLLWISRGKANREIGEILAISPRT
                     VNKHLEQIFVKLGVENRASAAARAVRALAR"
BASE COUNT         1437 a         2304 c         2405 g         1296 t
ORIGIN      
        1 cgctttattt cgaggccctt ggcgagcgcc tcgcgaacac cggtgacgat gacggtgtcc
       61 acttcttcgc ccgacggcgg ctcagcggca ggaggctgct gcgcgatggc ggagaccgaa
      121 atggaaagtg ccgccagcgc ggacgcagaa ctgatccgat tgataccagc cacgttgtac
      181 cccctctgac ggacgacctg ggctaaaggt gaggctgtcc gtttattgat tactcggaca
      241 aatgctagcg gcaggacaag cggcaatgca atgtccgccg tgtcatagct ccatgatcag
      301 actgatatgg atgtcagaca cttgggcgca ccctcattac tcggactttt gccaatcgtt
      361 gattagtgaa ggtgctttta tggatgaggc ggccgggccg gtgagccggg cgatatcaag
      421 ggtttgcgtg cagttttgcg tgtccgtcag acacctcgcc tgacacacgg gacagaggca
      481 aacaaccttg tcgccgggct ccgctgcgcg gacaatgcgg ccgcgcgcga gttacacggc
      541 cttctggaga ttttcgggaa tggcagtcaa caagaagaac aggaaggaca gcaggaaggg
      601 ccggaagcag gagacggctg gcgcgcatcc tcatcgcaag ccgcttcggt tgcacggcac
      661 catcgcgcgc gacctcggcg ttctcatcgt gtcgggccgc tatcgcgccg gccatgtgct
      721 cgacggggaa gtcgaagcga gcgagcagcg aaaggtgtcg cgcactgcat atcgcgaggc
      781 gctgcgaatc ctcgcggcca agggtctggt gcacagccga ccgcgcgtgg gtacgcgggt
      841 gagtccgctg gaaaactggc accttctcga tccggacgta ctttcatggg ccttttccgg
      901 tgaacccgaa ccggaagtgc tgcatggttt gttcgaattg agaaccatcg tcgagcccgc
      961 cgcggcggcg cttgccgcgt cgcggcgaaa tcagaagcac ctcgacagca tgcgtcgcgc
     1021 gctcgatgcc atgacggttc acacgctgaa cgtcgaagaa ggccgcatcg ccgacaagga
     1081 atttcacgcc gcgctgctgc tcgcgacggc aaatccattc atcgtgtcgc tgaccaacgg
     1141 cgtcaccgcg gccgtgaacg cgctcaccga gttcaagcag cgcatcgcac cgctcaagcg
     1201 cgatcccgtg cccgatcact ggcgtgtcta cgacgcgatc gccgcgaaag atgccgaagg
     1261 ggcgcgcgcc gcgatgaccg agctcattcg cctggccatc atggatacgc cgaccaagca
     1321 gcgtccgaaa cacgcggtgg cctcgcgcga ctcccgggcc gcgcacactt gagactgaag
     1381 tttgattcgc cgcgcgcggt tccgtcttgc gcgccgcgca gtttcgcggc tagcataaat
     1441 ttgtctgagt aatagccgca tcgagggggt tcgtgggtta tggacgcgga tggccgttcg
     1501 ctgctcgagg gcggcggcag caccgccatc cggggcaatg accgcttgcg aggcgcgggg
     1561 cacaatgcgg tgtctagtga cgacgcgcag gcggacggat ggccgcgcgc ccaaatccag
     1621 gagcagcaat gaccagaagc agaacgaagt ggttagtggc ggcggtgttt ggaacctgcg
     1681 tgatgatcgc gggctccgcc acggcacggg ccgcggaagc ggtcaacgta tcggtgacga
     1741 ttcgcgccga tcagccgggc gagaaaatca atccgaatgt ctacggccaa tttgccgaac
     1801 accttggcgc cggcatctac gagggcgtat gggtcggcga gaagtccacg atcccgaaca
     1861 cccacggtta tcgcaacgac gtcatcgagg ctctcaggaa tctcaaggtg ccggtggtgc
     1921 gctggcctgg cggttgcttc gccgatgagt accactggcg tgacggcatt ggcgatcgtg
     1981 ccaaacgccc ggtcaaggtg aacacgcatt ggggtggggt cgcggagacc aacgaattcg
     2041 gcactcatga attcatggcg ttcgccgaga tgatcggcac gaaggtctac atcagcggca
     2101 acgtgggatc tggcagcccg caggaaatgg ccgactggat ggagtacatg acgtcgaaca
     2161 cggtgtcgac gctcgccaac gaacgccgca agaacgggcg cgaacagccc tgggacggtc
     2221 actacttcgg catcggcaac gagacctggg gttgcggcgg caacatgcgt ccggagtact
     2281 acgccgacct gttccggcaa tacgccacgt atgtgaaggc gccgcgcggc aagcgcccgg
     2341 tgatcgtcgc gagcggcggc aatgacgagc acacgatctg ggccgaagtg ctgacgaaga
     2401 tggtggaacg cgacatggac gccatctcgc atcactacta caccatcccc ggcaaggaat
     2461 ggcggcagaa gggcaagtcg attggcttca ccgaagccga gtggatctcg acgctcaacc
     2521 acacgatgcg catcgacgaa tacatcacca acaacgtgaa gatcctcgac cagaacgatc
     2581 cgaagggaaa ggtggcgttc tacgtcgacg agtggggtac gtggtacgac ccggagccgg
     2641 gccgcgagcc gggtttcctc tggcagcaca acacgctgcg tgatgcgctg gtcgcggggc
     2701 tcaacttcaa catcttccat cgtcacgcca agcgtgtgca gatgaccaac atcgcgcaga
     2761 tggtcaacgt gctgcaggcc atgatcctca ccgaaggtcc gaagatggcg ctcacgccta
     2821 cctaccacgt gttccagatg tacatcccgt tccagggggc tacctacctg cccacggaga
     2881 tcaccacgcc cgagtacaag cttgcggact acacggtgcc ttcggtgagc gtctccgcgg
     2941 cgcgtgatac cgacggacgg ctgcagctcg cgctggtgaa cctcgatccc catcgcgagg
     3001 cggtggtgac gacatcgctg acgggtgcga aggcgagtgc ggctctcggc cgcgtgctca
     3061 cgtcgaagac catggatgcg cgcaacacag tggacgcgcc agaggcgatc acgccggtga
     3121 agatcagcgc gacccgcaag ggcgacgcgc tggtgctgcg cttaccgccg aagtcggtga
     3181 gtgtggttcg gcttcaatag acgcgaagac caaagcctga tcgcggccac gccggccgcg
     3241 atcagcgccg cgcccgaaaa caggaaaccg agagcgtgct gccagctcag ctggtgcatg
     3301 acttccccga agaaggtcga ggtatcgccg tggccgggat gggccagcgc gaacgctggc
     3361 agaaacgccg cagccacggt gccaattcga gccaggtgtt ttcgcttgat ggtcatgggc
     3421 caaagattac cccaaggccg cacggctcca ctcttatata agtcatttga cgcagggcac
     3481 cagcgggccg ccgggtatca tccttagccc atgagcacca aatcagatgt attgcgggtc
     3541 ggcgtgggcg gccccgtggg ttcggggaag accgcgctcg tggattcact gtgcaaacgc
     3601 atgcgcgacg atttcaatat cgccgtcgtc accaatgaca tctacaccaa ggaagaccag
     3661 gaattcctga tccgcagcca ggcgctcgcg cccgagcgca ttcgcggcgt cgaaaccggc
     3721 ggttgcccgc acacggcgat ccgcgaagac gcctcgatga atctcgcggc catcgaccaa
     3781 ttgatgcacg acttcaccgg actcgatttc atcatcgtcg agagcggcgg cgacaatctc
     3841 gccgcgacgt tcagcccgga gctctcggat ctcacgatct acgtcatcga cgttgcagcc
     3901 ggcgacaaga ttccgcgcaa gggcgggccg ggcatcatga aaagcgatct gctcatcatc
     3961 aacaagatcg acctcgcgcc ccatgtgggt gcgtcgctcg aggtcatgga tcgcgatgcg
     4021 aaaaagatgc gtggcgagcg gccgttcgtt ttcacaaatc tgaaaaccgg ccaggggctc
     4081 gaccaggtgg tccagttcat cgaaaaacag gggatgctgc gacactgaca atcgccggtt
     4141 gtttttcctg acagccgcgc gactacgcag cgacgagact cagcacgccg ggcagaccgg
     4201 cgccacgcgg aggcctcatg cagggtcaac ccttcagagt cctgtttctc tcgcagcgca
     4261 attccgcgcg cagcatgatg gccgaggcca tcgccaatac gatcggccac gggcagttgc
     4321 aggcgttcag cgccggcgtc cggcccgcgt cgcgtatcga tcccattgcg atcgaattgc
     4381 tggaacacgc aggagtacag gcaccgtcac atacgccgca gcacgtgcgt gaattcagcg
     4441 cgcccgaatc accgccgctc gatttcgtct tcacgctgag cgacaccgcc gccggtgagg
     4501 ggccgccttc gtggcccgga catccgatca ccgcgcattg gcggtgtgag gatccggagc
     4561 aattcgacga cccggtggaa aagcgtcaat cgctgatccg cactcgtgcg gaactcgagc
     4621 gccgcctgcg cgtgctcacc aatctaccgg tccggtcact tgaccggctc agtctccaga
     4681 cccaggtgga ctcgctgggg cgcaccggcg catcctgacg ccgatcggtc gtctatcgcg
     4741 gaactcgcgg ttcggacgtc agtccgaatg cgcgagggct catgcgaaca aacatcctgt
     4801 ctatctgcgt gctgctcggc gtggcgccgg tgctggcgca atcgccggaa tccgagctgc
     4861 cgctcgatga agtcgtggtc accggcgaat atcccggccc cggcatgtgg aaagtcacgc
     4921 gcgctgacga tgcccgtggc cacgtgatgt ggatcgtcgg cgatccgccg ccgttgccga
     4981 agcgcatgaa atggaagtcg aaggatgtcg aggccgttgc gctcggtgcg caggaaatcc
     5041 tgcgcgattc ttcggtcagc atggagcccg acgagaagat cgggttcttc cgcggaatga
     5101 cgctgctgcc ggcgatgctg gaagcccgcc gaaaccccga cgatgccaaa ctgaaagacc
     5161 tggtgccgcc ggatctctac gcgcgatggc tggtacagaa gaagctctac ctgggccgcg
     5221 aaagcggcgt ggagagctgg cgaccgctgt tcgcggccga caagctgcgc aaggccgctt
     5281 acgacgatct caagctgcgc gaaagtggcg cggtgtggga agtcatcggc aagctggcgg
     5341 agaagcacaa gatcaaggtc acttcaccga cgctgcgttt caccatcaag cggagcgaag
     5401 tccgcaccaa gatcaaggaa ttttcacgcg agtcgctgcc cgacgtggag tgtttcgaaa
     5461 cgatgttgcg gctcaccgag gcgctgggcg atcgcgagac gcaaaatgcg cgtgcccacg
     5521 cgtgggccac cggcgatgtc gaaggcctca cgcggctgcc ggtgttgccc agcccctatc
     5581 tgccgtgcgc gatggcggtc atgaactcgc aggtggcgcg cgaggtcata cccgccgaca
     5641 tccgcgaaaa ggtccatgcc ttgtggctcg aggccgcgga aaagagcctc gcggcgaaca
     5701 cgacgacgtt cgccatcgtt cccttcgtga aactcacgcg tgccgacggt tatctcgcga
     5761 agttgcgcgc gaagggctat ctcatcgagg cgccgcaata accgcaaagc tcagcgcggc
     5821 cgttccacct tggccacggc gagcgcttca ttgagtattc gcgcccattt cgcgaaagcc
     5881 gtgcgctgct cgtgggcgtt cgtcacgcgg ttggcgagcc gcagttcgtt gaaggcatac
     5941 cgctcattgc cttcgtagat cgtcaggcgg cccacgagtt ttcccgtggc cgcgtcgcgc
     6001 aattcgccgg tcaccttcat cttgacgggg ccggtggtgt actggcgttc gccgggcgcg
     6061 gtgctcgcgt ccggggccac gatgtccaga tcctggatcg ccgggttcac gatcaatacg
     6121 tccggcgcaa gttcgtccgc atattcgtag ggaccgcgat cgatgatctc gcgcgtgaat
     6181 tcctcgcgga acagttcggc cgattccctg acgatgcgcg cgtattcagt gtcgttgacg
     6241 tccgagtggt tcttccgcca gtcgcgcacg aattcgatgg tcggcggctc gagcatgatg
     6301 cgctggtact gcgtgaaggt ggcgccgggg gcccggtaga caccggccac gcggcgcgag
     6361 gggacgcgga cgagtccgtc gtcggtcagt tcttccggac cgcgtgacgc ggtgcatccc
     6421 gcggacaacg cgcaactcaa ggccagccct attgcgcagg cacgtaacca catttgtctt
     6481 gcccctcgcc agacgacgtg aggcgccgat tctacgcccg gttagcgcgc cagcgcgcgc
     6541 acggctctcg cggccgcgga cgcgcggttc tccacgccca gcttgacgaa gatctgctcc
     6601 aggtgtttgt tcaccgtacg gggactgatc gccagtattt cgccgatctc gcggttcgcc
     6661 ttgccacggc tgatccacag cagcacttcc gattcgcgcg acgtcagcga cagcgtctgc
     6721 tgcaattgct gttcgtcggc cgtggtgctg agctcggtca gccggaacag caattcgtcg
     6781 gggccgatgg ggctcagcac cgaaatctcg accttgcgtc cattgatgtc gacgagcagg
     6841 cgcgtgttgc cgccttcgga ccgcaattgc atgagctggc ccatcatcgc cgagggcagg
     6901 ctcgcgttgc tctcgctggc ggcgggaaac agctccgcga gcaactggcg cgctttcggc
     6961 gtgcaccaga gcaggcggcc cgcgcggtcg gtcgccagca ggaatcggcc cgtggcatcg
     7021 agcgcggcgc gtgtaccggc cgcgacacgc gcattggcga gatgcacccg gatgcgcgcc
     7081 agcagttcgt ccacgacgag cggcttggtg acgtagtcga cgccaccggc cgcgaggcct
     7141 tcgagcacgt tctcggtttc ggtgaggccg gtcatgaaga tcaccggcag atgcgccagc
     7201 atcttgttgc gcttcatgcg ccggcaggtt tcgaagccgt tgaggcccgg catgacggcg
     7261 tccatgagca cgagatccgg ggtgatctgc tcgataagcc gcaacgcgct ctcgccgtcg
     7321 gtggcgatca gcgccgtgaa acccgcgtga tcgagcgtgt cggtgagaaa gcccagcgtt
     7381 tcgggggtgt cgtcgacaac caggatggtg tcgcgacgca catcggattg atgagacgtg
     7441 gt
//
LOCUS       DATDEU010000571         8805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2154506, whole genome shotgun sequence.
ACCESSION   DATDEU010000571 DATDEU010000000
VERSION     DATDEU010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154506"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VIV63_13630"
     CDS             complement(<1..1082)
                     /locus_tag="VIV63_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY6125687.1"
                     /translation="MGILALTLAASQVPAQTAVEAGHDGAGNRWVATWGSAPIAPGVT
                     TIDALFGADTSRSFENQTVRNIVHTSVGGRRVRIRLSNAFGMLPLRVGAAQVALSSAD
                     AAVYPGTGRRLTFGGQNSVLIPTGGVMVSDPADLDVPADGNLAVSVYLPGITEPATFH
                     EFTLQTSYVTAENSGNLTAAADLPGAATTPATFYLSVVEVLPWDPVGAVVAFGDSIAQ
                     GGGSTRNQNRTWPDKLSDRLNGNPYRPRLAVVNQGVGCGRLLYDLCGPNGIARFDRDV
                     LGVAGVRSVIVSLGLNDIMIPSTLPMFGKPEFAAESVSATDIINGLKQLVMRARANNV
                     RIIGATITPFGSSTVPGVFTPETEAKR"
     gene            complement(1233..1952)
                     /gene="phoU"
                     /locus_tag="VIV63_13635"
     CDS             complement(1233..1952)
                     /gene="phoU"
                     /locus_tag="VIV63_13635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY6125688.1"
                     /translation="MSLSLEGHTARAFDGDLSSLHVQAVAMGALVIDQVQLAVNAYSG
                     WDRSMATLVLERERKINLYDAEIEEAALQVIARRQPVAQDLRAVFAIDKVVTELERAA
                     DEAKKLALAVLADSESNGFRPGAATAREARQLGRLAVLMIRASLEAFDRLDPVAAAAV
                     IHQDQEMDSEYAAALRRILTRAMEDVRQTQAAIEAAFIMRSLERIGDHARNIARHVGF
                     IGGQFDPASTGVLPSSLTVKN"
     gene            complement(1967..2734)
                     /gene="pstB"
                     /locus_tag="VIV63_13640"
     CDS             complement(1967..2734)
                     /gene="pstB"
                     /locus_tag="VIV63_13640"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY6125689.1"
                     /translation="MAVDDPIFSCRDVDVYYGAKHAIKKVGIDVGKKQVLAMIGPSGC
                     GKSTFLRCLNRMNDTIEGARVEGSILLDGKDIHDKKQDVVQLRARVGMVFQKPNPFPK
                     TIYENVAYGPRIHGLAGDRVELDEIVHGSLQRAGLWEEVKDRLQAPGTSLSGGQQQRL
                     CIARTIAVNPEVILMDEPCSALDPIATARIEDLMEELRQSYAIVIVTHSMQQAARVSQ
                     RTAYFHLGDLIEVGETDRIFTNPRHKLTEDYITGRFG"
     gene            complement(2853..4160)
                     /gene="pstA"
                     /locus_tag="VIV63_13645"
     CDS             complement(2853..4160)
                     /gene="pstA"
                     /locus_tag="VIV63_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY6125690.1"
                     /translation="MNSTNKPRHTPAETQAIVERSLRRRYWAERRFRAYGFTAVTAGM
                     AFLVYLFFVIFSNGIGAFQQTQMRVSVYYDPQIIDPTGNRNPDELQAADYPAVVRAAL
                     KAKFPEVEGRAATRELTRLISGSAAFDLQKRVISDPSLIGTTEERWLLASDAVDLLQK
                     DHIDRNIPEAERPLSDAQMAWTDKLRAEGSLALKFNKTFVTGGDSREPENAGIWGAVV
                     GSFFMLALTLLLSFPIGVATAIYLEEFARRNRWTDLIEVNINNLAAVPSIVFGLLGLA
                     VFIGFFGLPRSAPLVGAMVLTLLTLPTIIIASRAALKAVPPSIREAALGMGASKIQMV
                     AHHVLPLAMPGMLTGTIIGMARALGESAPLLMIGMVAFIVDIPKGVTDAATALPVQVF
                     LWSDSPERGFVEKTSGAIIVLLAFLVVMNTTAVILRKRFERRW"
     gene            complement(4144..5532)
                     /gene="pstC"
                     /locus_tag="VIV63_13650"
     CDS             complement(4144..5532)
                     /gene="pstC"
                     /locus_tag="VIV63_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY6125691.1"
                     /translation="MTPAALLIALTLLSVGAFWIGKSRSLALVGGTRGVRHLHSLPAH
                     YGLMTAMWCALPALAVIGIWLVFQDAIILRLVVDHLPAGVRDLPANELSLVMNDVRNL
                     IAGNVPASSVNESVRAASAEYIHLRGISRMALTVLVMVMSIAAIIVIRSRITPELRAR
                     NRVERVIEWILIGCSTIAVFTTLGIVASVAFEAFRFFAMVSPIDFLFGTTWSPQMAIR
                     ADQVGSSGSFGAVPLFAGTALISFIAMVIAVPIGLFSAIYLAEYAHPKFRTVAKPLLE
                     VLAGVPTVVYGFFAALTIGPALRGWGEGFGLNVASESALAAGFVMGIMIIPFVSSLAD
                     DMISAVPQSLRDGAYALGATRSETIKQVVLRAALPGIVGGVLLAVSRAIGETMIVVMA
                     AGLAANLTANPLEAVTTVTVQIVTLLVGDQEFDSPKTLAAFALGLVLFLVTLALNVLA
                     LHIVRKYREQYE"
     gene            complement(5611..6975)
                     /locus_tag="VIV63_13655"
     CDS             complement(5611..6975)
                     /locus_tag="VIV63_13655"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEY6125692.1"
                     /translation="MKRNLISLAVASVLAGASPAFAQSATEIDALKAQLSALAAKIEQ
                     LEKAQTQTNKTVNETIATADKTADVVAQERSRLTFNGDLRYRNESFDVEYVERNRNRD
                     RVRARLNAAYRVNDTLTGVFGIATGGPDPRSSNQTLTDQNSRKDFELDLGYIQWAPNA
                     DWRITAGKQRYPWQRTASLFFDGDVNPEGIAVNYTKGNFFTGVFYDWLGERALSFSNV
                     TTGTNVDSIMYGGQVGYRIPFSDAVKLTVAGTYLNFDGVQGYNPFFGGSSFGNTTTTS
                     AAVCNRSLGAGVACLLSDYDIIEGFADLTATVGGRPLRVFVDYAQNTEAEVNPVAGEK
                     LDTAMAYGISYGAATANKGTWEFGVIQQTIEKDALFGQLIDSDFGDGNTDTDGLVLRG
                     AYTVARNWTINATLFLNELSNDVPQSVTVFNENTAAPYDTTVLTGITDREYKRLQLDL
                     NFRF"
     gene            complement(7073..8125)
                     /locus_tag="VIV63_13660"
     CDS             complement(7073..8125)
                     /locus_tag="VIV63_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY6125693.1"
                     /translation="MTRNILISSLAASIVCAMSTTAQAQSARDSINIVGSSTVYPFTT
                     TVAEQFGRAGKFKTPKVESTGTGGGIKLFCNGVGPQYPDIANASRRMNVNEFAACQKN
                     GVKDIVEVKVGYDGIVLAQSKAGATLTPTRKEVYLALAKNVPDPANPSNLIPNPYVTW
                     KDINKSLPAVKIEVLGPPPTSGTRDSFVELYMEAGCRTFAWLNGLRAQDEPRFKRACD
                     TVREDGSYVEAGENDNLIVQKLTANKDAVGIFGYSFLEENLDKLKGAVVDGVSPSYES
                     VSSGKYPASRPLFIYVKKQHINVIPGIAEFVAEYTSEKALGEEGYLADKGLVPPLKNE
                     IGKIRDDAKSMKPLKL"
     gene            8260..>8805
                     /gene="dapE"
                     /locus_tag="VIV63_13665"
     CDS             8260..>8805
                     /gene="dapE"
                     /locus_tag="VIV63_13665"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005169709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY6125694.1"
                     /translation="MSATVDLTLDLLGRNSVTPADQGCQEVMTRRLAEAGFAIENLRY
                     GNVDNFWATRGKGGPVLCFAGHTDVVPTGPLEEWKSDPFKPAVRDGLLFGRGAADMKS
                     ALAAMVTATEEFVKAHPKHKGTIAYLITSDEEGPSVDGTKKVVETLRERGQKIDWCIV
                     GEPSSEKELGDTIKIGRRGSLS"
BASE COUNT         1628 a         2739 c         2827 g         1611 t
ORIGIN      
        1 cgcttcgctt ccgtctcggg agtgaagacg ccgggcaccg tgctcgagcc gaaaggcgtg
       61 atggtcgcgc cgatgatcct gacgttgttc gcgcgagccc gcatcaccag ctgcttgagc
      121 ccattgatga tgtccgtggc gctcacgctc tccgccgcga attccggctt gccgaacatc
      181 ggcagcgtcg acggaatcat gatgtcgttg agcccgaggc tcacgatgac cgagcgcaca
      241 cccgcgacgc ccaatacgtc acggtcgaaa cgcgcgatac cattgggccc gcagaggtcg
      301 tacaacaagc ggccacaacc aacaccctgg ttcaccaccg cgagccgcgg ccggtacgga
      361 tttccattga gccggtcgga caacttgtcg ggccaggtgc gattctgatt gcgcgtggag
      421 ccgcctcctt gcgcaatgga atcgccaaag gcgacgaccg cgcctaccgg atcccagggc
      481 aacacttcga ccacgctcag gtagaacgtt gcgggcgtcg tcgccgcccc gggcaaatcc
      541 gccgcggccg tcaggttgcc ggagttctcc gcggttacgt acgacgtctg cagcgtgaac
      601 tcgtggaagg tcgcgggctc ggtgatcccc ggtagataga cactgaccgc caggttgccg
      661 tcggcgggca cgtccagatc ggcggggtca ctcaccatca cgccacccgt agggatcagc
      721 acggagttct ggccaccgaa agtcaggcgg cgcccggtgc cgggatacac cgcggcatcg
      781 gcgctgctca gcgccacctg cgcggcaccc acgcgcagcg gcagcatgcc gaaggcattt
      841 gacaaccgga tccgcacccg tcgcccaccc acgctcgtgt gcacgatgtt gcgcaccgtc
      901 tggttttcga acgagcggct ggtgtcggcg ccgaacaacg cgtcaatggt tgtcacaccc
      961 ggagcgatgg gcgctgaccc ccaggtcgcg acccagcggt tccctgcccc gtcgtggccc
     1021 gcttcgacgg cggtctgtgc ggggacctgc gacgcggcga gcgtgagcgc cagtattccc
     1081 atcaggcttc tcatgcggat cattggactc tccttgacgc aatggacgat gcgtggagtc
     1141 tgagaagcag cgcaggctta aggtagaacc agttgcgcgc aatccgatga agccagttct
     1201 ctagatgggg acatgccccg ataccggact aactagtttt tgacggtcag cgaggatggc
     1261 aggacaccgg tggaagccgg atcgaactga ccgccgatga aacccacgtg ccgcgcaatg
     1321 ttgcgcgcgt ggtcaccgat gcgttcgagc gagcgcatga tgaatgcggc ctcgatcgcc
     1381 gcctgcgtct ggcgcacgtc ttccatggcg cgcgtaagta tccgtcgcag cgccgccgca
     1441 tattcggaat ccatttcctg gtcctggtgt atcacggcgg ctgccgctac aggatcgagc
     1501 cggtcgaagg cttcgagcga tgcgcggatc atcaacaccg cgagacggcc cagttgccgc
     1561 gcctcgcgcg ccgtggcggc gcccggacga aatccgttcg actcgctgtc cgccagcacg
     1621 gccagcgcca gcttcttggc ttcgtcggca gcgcgttcga gttcggtcac caccttgtcg
     1681 atggcaaaca ccgcgcgtag atcctgcgcc accggttgcc gccgggcgat gacctggagc
     1741 gcggcctctt cgatctcggc gtcgtaaaga ttgatcttgc gctcacgctc gagcaccagc
     1801 gtggccatcg aacgatccca gccagagtat gcgttgaccg cgagttgcac ctggtcgatg
     1861 accagcgcgc ccatggcgac cgcctgcacg tgcagcgacg acaggtcgcc gtcgaatgcg
     1921 cgggcggtgt ggccttcgag actcagtgac atcgtaatct gatgcgtcag ccaaaacggc
     1981 cggtgatgta gtcttcggtc agtttgtggc gcgggttcgt gaaaatgcgg tccgtctcgc
     2041 ccacttcgat gagatcgccc aggtggaagt acgccgtccg ttgggaaacg cgggcggcct
     2101 gctgcatgga gtgcgtgacg atcacgatcg cgtagctctg gcgcagttct tccatgaggt
     2161 cttcgatgcg cgcggtggcg atgggatcga gcgccgagca cggctcgtcc atgagaatga
     2221 cttcgggatt cacggcaatc gtgcgcgcga tgcacaggcg ctgctgctgg ccacccgaca
     2281 acgaggtgcc cggcgcctgc aggcggtcct tcacttcttc ccacaatccg gcgcgctgca
     2341 acgaaccgtg cacgatctcg tctagctcga cgcggtcgcc ggccagcccg tggatgcgcg
     2401 gaccataagc gacgttttca tagatggtct tcggaaacgg attcggcttc tggaacacca
     2461 tgccgacgcg cgcacgcagc tgcaccacgt cctgcttctt gtcatggatg tccttgccgt
     2521 cgagcaggat cgacccttcg acgcgcgcgc cttcgatggt gtcgttcatg cgattgaggc
     2581 agcgcaggaa cgtggacttg ccgcaacccg acggaccgat catggcgaga acctgcttct
     2641 tgccgacgtc gatgcccact ttcttgatgg cgtgcttcgc gccgtagtac acgtcaacgt
     2701 cgcggcagct gaagatggga tcgtcgacgg cgatgttacc gacggttgcc ctgctcgaat
     2761 ccgttggctg ctccagcgaa acgggctccg aggtccggcc gctcggcttc aggttgattc
     2821 cagctcgttc cacttgtgcc gcttccataa tttcaccaac gtcgttcgaa tcgtttacga
     2881 aggattaccg ctgtcgtgtt catgaccacg aggaaagcca ggagcacgat gatggcgccc
     2941 gaagttttct cgacgaagcc gcgctcggga ctgtcggacc acaggaacac ctgcacgggc
     3001 agcgcggtcg ccgcgtccgt gacgcccttg ggaatatcga cgatgaacgc caccatgccg
     3061 atcatgagca gcggcgcgga ttcacccagc gcgcgcgcca tgccgatgat ggtgccggtc
     3121 agcatgccgg gcatggcgag tggcaacacg tgatgcgcca ccatctggat cttggatgcg
     3181 cccatgccga gcgccgcttc gcgaatcgac ggcggcaccg ctttgagggc cgcacgcgac
     3241 gcaatgatga tggtgggcag cgtcagcagc gtgagcacca tcgcgccgac cagcggcgcc
     3301 gatcgtggca agccgaagaa tcctatgaag accgcgagac ccaacaaccc gaacacgatc
     3361 gacggcaccg ccgccaggtt gttgatgttg acctcgatga gatcggtcca gcgattgcgg
     3421 cgcgcgaact cctcgaggta gatcgccgtg gcgacgccga tggggaacga cagcagcagc
     3481 gtcagcgcca gcatgaagaa tgagccgacc accgcgcccc agattccggc gttttccggc
     3541 tctcgcgaat cgccgccggt cacgaacgtc ttgttgaact tgagcgcgag cgaaccttcg
     3601 gccctgagtt tgtcggtcca cgccatctgc gcgtcgctca agggacgctc ggcctcggga
     3661 atgttgcgat cgatgtggtc cttctgcaga agatccaccg cgtcgctcgc gagcagccag
     3721 cgttcttcgg tcgtgccgat caacgaagga tcggaaatga cccgcttctg cagatcgaat
     3781 gcggcgcttc cgctgatgag gcgcgtgagc tcgcgcgtgg ccgcgcggcc ttcgacctcg
     3841 gggaatttcg ccttgagcgc tgcgcgaacc accgctggat agtcggcagc ctgcaactcg
     3901 tcgggattgc gattgccggt cggatcgatg atctgcgggt cgtagtaaac ggacacgcgc
     3961 atctgcgtct gctgaaacgc accgataccg ttcgagaaga tgacgaagaa cagatagacc
     4021 aggaacgcca tgccggccgt cacggccgtg aagccgtagg cgcggaaacg acgttcggcc
     4081 cagtagcgac gccgcaggct gcgctcgacg atcgcctggg tttccgcggg tgtgtggcgc
     4141 ggcttattcg tactgttcac gatatttcct gacgatgtgc agcgcgagca cgttgagcgc
     4201 cagcgtgacg aggaacagca ccaggccgag tgcgaacgcc gccagcgtct tggggctgtc
     4261 gaactcctgg tcgccgacga gcagcgtgac gatctgcacc gtcacggtgg tcacggcttc
     4321 gagaggattc gccgtgaggt tggcggcaag accggccgcc atcaccacga tcatggtttc
     4381 gccgatggcg cgcgagacgg ccagcaacac gccaccgaca atgcccggga gcgcggcgcg
     4441 cagcaccacc tgcttgatgg tctccgaacg cgtggcgcca agcgcataag caccatctcg
     4501 caaagattgg ggcacggcgc tgatcatgtc gtcggcgagc gaggacacga acgggatgat
     4561 catgatgccc atgacgaagc ccgcggcgag cgcgctttcc gacgccacgt tcaggccgaa
     4621 gccctcgccc cagccacgca gcgcgggccc gatggtcagg gcggcgaaga agccgtacac
     4681 caccgtaggc acgccggcca ggacttcgag cagcggtttg gccaccgtgc ggaacttcgg
     4741 atgcgcgtac tcggccaggt agatagcgga gaacaggccg atgggcacgg cgatgaccat
     4801 ggcgatgaaa gagatgagcg cggtgccggc gaacagcggg acggccccga acgagccgga
     4861 cgaacccacc tggtcggcgc ggatcgccat ctgcgggctc cacgtggtgc cgaacaggaa
     4921 gtcgatcggc gacaccatgg cgaagaagcg gaaggcctcg aaggccaccg aggcgacgat
     4981 gccgagcgtg gtgaacaccg cgatggtcga acagccgatg agaatccact cgatgacgcg
     5041 ttccacgcga ttgcgtgcgc gcagctcggg agtgatgcgt gaacggatca cgatgatcgc
     5101 ggcgatcgac atgaccatca ccagcacggt gagcgccatg cggctgatgc cgcgaaggtg
     5161 gatgtattcg gcggaggcgg cgcgcaccga ttcgttgacc gagctcgccg gcacattgcc
     5221 cgcgatcagg ttgcgcacgt cgttcatgac cagcgacagc tcgttcgccg gaagatcccg
     5281 caccccggcg ggcagatgat cgacgaccag gcgcaggatg attgcgtcct ggaagacgag
     5341 ccagattccg atgaccgcga gtgccggaag cgcgcaccac atggccgtca tcaagccgta
     5401 atgagcggga agcgagtgca gatgccgcac gccgcgcgta ccccccacca gggccagcga
     5461 acgggacttg cctatccaga aagcgcccac tgacagcaaa gtcagggcga tgagtagtgc
     5521 cgcgggcgtc attggggttc ttatttagag aagctttgtt acagcttcgt gaaaaaggag
     5581 gggcggcttc cctgccgccc cgaggctctc ttagaaccgg aagttcagat ccagctgtaa
     5641 gcgcttgtat tcccgatcag taatgcccgt cagcacggtc gtgtcatacg gggcggcagt
     5701 gttctcgttg aacacggtca ccgactgggg aacgtcgttg gacagctcgt tcaggaacaa
     5761 cgtggcgttg atcgtccagt tgcgggccac ggtgtaggca ccgcgcagaa ccaggccgtc
     5821 ggtgtcggtg ttgccgtcac caaagtccga atcgatgagc tggccgaaca gcgcgtcctt
     5881 ctcgatcgtc tgctggatca cgccgaactc ccaggtgccc ttgttggccg tcgcggcgcc
     5941 atagctgatg ccgtacgcca tcgcggtatc cagtttctcg ccggcgacgg gattcacttc
     6001 cgcctcggtg ttctgcgcgt agtcgacgaa cacgcgcagc ggccggccgc ccaccgtggc
     6061 cgtcagatcc gcgaagcctt cgatgatgtc gtagtcggac agcagacagg cgacgccggc
     6121 accgagcgag cgattgcaga ccgcggcgct ggtggtcgtg gtgttgccga aggagctgcc
     6181 accgaagaaa gggttgtagc cctgcacacc gtcgaagttg aggtaggtgc cggcgaccgt
     6241 gagtttcacc gcgtccgaga acggaatgcg atatcccacc tgcccgccgt acatgatgga
     6301 gtcgacgttg gtgccggtgg tcacgttgct gaacgacagc gcacgctctc cgagccagtc
     6361 gtagaacaca cccgtgaaga aattgccctt ggtgtagttg acggcgatgc cttccggatt
     6421 gacgtcaccg tcgaagaaca ggctggcggt gcgctgccac ggataacgct gtttgcccgc
     6481 cgtgattcgc cagtcggcat tcggcgccca ctggatgtaa cccaggtcga gctcgaagtc
     6541 cttgcgcgaa ttctgatccg tgagcgtctg gttggacgaa cgcggatccg gaccacccgt
     6601 ggcgatgccg aacacacccg tgagcgtgtc gttgacgcgg tacgcggcgt tgaggcgtgc
     6661 gcgcacgcgg tcacgattac ggttccgttc cacgtattcc acgtcgaacg attcgttgcg
     6721 ataacgaagg tcgccgttga atgtcaggcg cgaacgctcc tgggccacga cgtccgccgt
     6781 cttgtccgcg gtcgcgatcg tttcgttgac cgtcttgttg gtctgcgtct gcgctttctc
     6841 gagctgctcg atcttggccg ccagcgcgga gagctgcgcc ttgagcgcgt cgatctcggt
     6901 ggcgctctgc gcgaatgccg gcgatgcacc tgccaggacg ctggcaacag ccagggagat
     6961 taaattgcgt ttcatgagat gttctctaac tactttggaa tgacgtttgt cttacgcggc
     7021 aaaggcggtt ccgcgcggaa ctccggggtt ccgcgcggcg ccagtgcgct gatcagagct
     7081 tcaggggctt catcgatttc gcgtcgtcgc gaatcttgcc gatctcgttc ttgagcggcg
     7141 gcaccaggcc cttgtcggcc aggtaacctt cttcgcccag cgccttttcg ctggtgtatt
     7201 cggcgacgaa ttcggcgatg cccgggatga cgttgatgtg ctgcttcttc acgtagatga
     7261 agagcgggcg cgacgccgga tacttgcccg acgaaacgct ctcgtagctc ggcgacacac
     7321 cgtcgaccac ggcacccttg agcttgtcga ggttctcctc gaggaagctg tagccgaaga
     7381 tgccgaccgc atccttgttg gccgtgagtt tctgcacgat gaggttgtcg ttctcgcccg
     7441 cttcgacata ggagccgtct tcacgcacgg tgtcgcaggc gcgcttgaag cgcggttcgt
     7501 cctgggcgcg caggccgttg agccaggcga aggtgcggca acccgcttcc atgtacagct
     7561 cgacaaagga gtcgcgcgtg ccggaagtcg gcggcggtcc gagcacttcg atcttcaccg
     7621 cgggaagcga cttgttgatg tccttccagg tgacgtacgg attcgggatc aggttcgacg
     7681 ggttcgccgg atccgggacg ttcttcgcga gcgccagata cacttccttg cgcgtgggcg
     7741 tgagcgtggc gccggccttg ctctgagcga gcacgatgcc gtcgtaaccc accttcactt
     7801 cgacgatatc cttgacgccg ttcttctggc aggccgcgaa ctcgttcacg ttcatgcggc
     7861 gtgaggcgtt ggcgatatcg gggtactgcg gaccgactcc attgcagaac aacttgatgc
     7921 cgccaccggt gccagtgctc tcaaccttgg gtgtcttgaa cttgcccgcg cgaccgaact
     7981 gctcggcgac ggtcgtggtg aacgggtaca cggtggacga acccacgatg ttgatggaat
     8041 cgcgcgccga ctgagcctgc gcggtggtgc tcatggcgca gacgatgctg gccgcgagcg
     8101 acgaaatcag aatattgcga gtcactgtat ttgcctccgg tggtgacggg cggcaattta
     8161 tgactgcaag gttgcacaaa tatttcagcg cgcccgtttt cacggaaccg tcataaaact
     8221 ccttgctgca caggtcccag ttccgtataa cgcgctgcga tgtcagccac cgtcgatctc
     8281 acgctcgatc tcctcgggcg aaactccgtt accccggccg atcagggttg ccaggaagtc
     8341 atgacacgcc ggcttgcgga ggcgggtttc gccatcgaaa acctgcgtta tggcaacgtc
     8401 gacaacttct gggcgacgcg cggcaagggc ggtccggtcc tgtgcttcgc gggacacacc
     8461 gacgtcgttc cgaccggacc tctcgaagaa tggaaaagcg atccgttcaa gcctgcggtc
     8521 cgtgatgggc tgctgtttgg ccgcggcgcc gccgacatga agagcgcgct ggccgccatg
     8581 gtcacggcca ccgaagagtt cgtgaaggcg cacccgaagc acaaagggac catcgcctat
     8641 ctgatcacca gcgacgagga aggcccctcg gtcgacggca ccaagaaggt ggtcgagacg
     8701 ctgcgcgaac gcggtcagaa gatcgactgg tgcatcgtcg gcgaaccttc gagtgagaaa
     8761 gaactcggcg acacgatcaa gatcggccgt cgcggctcac tctcc
//
LOCUS       DATDEU010000572         3222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_842711, whole genome shotgun sequence.
ACCESSION   DATDEU010000572 DATDEU010000000
VERSION     DATDEU010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842711"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..956
                     /gene="radC"
                     /locus_tag="VIV63_13670"
     CDS             249..956
                     /gene="radC"
                     /locus_tag="VIV63_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY6125695.1"
                     /translation="MDREVSAGDSGNISHWPRGERPRERLIERGAQALSDAELLAVVL
                     GTGSGGANALDIARQLLQHFGGSLRRLLTSDAENLLALRGLGPARYATLMASLELARR
                     HYCEALRAGPALNAPAATRQFLTAQLRDRPYEVFCCLHLDNRHRLIHFEEVFRGTIDG
                     ASVHPREVVRQALQYNAAALIFAHNHPSGVAEASQADELITRRLRDALALVDIRVLDH
                     LIVGDNNCLSFAERGLL"
     gene            1094..>3222
                     /locus_tag="VIV63_13675"
     CDS             1094..>3222
                     /locus_tag="VIV63_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125696.1"
                     /translation="MSRGLVWAALCATGLSACSGGGGSKPPPPPAGDTVAPETSFLST
                     PPALTNATVLVFGLTSNETGVTFEVLAGLPNYTTFPPLFGLTGLPDGPHTISVRARDA
                     AGNVDATPATYNFVIDTQAPDTTLATSPAAVTASTSATFTFTSEANATFEASVDGAAF
                     ATAASPLALSGLANGAHTVQIRARDAATNVDASPASFSWQVDTAAPAVSIVFPTRHSY
                     TEANSLHVRGTSSDISTITAVSVNGVAATTSDAYAHWAAVVPIAPGNNVLNVSVTDSL
                     GNTSASAARADVANRGVAISNIDSVTWDPVRGRAIVADNDRGVLIAIDGTTGNASIIS
                     GAARGTGPALGGSYFFAKMDTANDRILVMRGQSLIEVDPDSGDRAEIPTAAPDAHTTF
                     FGSTIACNADCSLLFLAQMSDPLNGGQGIFSISPQTGARTLITGGWPIVGSGYTIQTA
                     RGIAFDAVNSRIYVVDGSRNGLFAVNPATADRALISSSQPPAFGSGVDLVNPRGIVLD
                     LANNRAIVTDTLRNQQARLVAIDLSTGARSVFATTIVDATLAPATDMLLDASGSRMLI
                     GMNRGSIAQVSLTSGDVRRFSDSNVGTGMSLWGWGALLFEPANRTLLNAATGAIVRID
                     PATSDRSVVVPTLLASGTVPYAPSHLNVDTRAGAPPNRLIGVGPGSSPTMFRAYDLSA
                     GTDTEIVATSLPYGSAVFPFDAPNGRM"
BASE COUNT          547 a         1137 c          999 g          539 t
ORIGIN      
        1 agcatcgcga acaagcagcg cgtgagaatt ttcatagagc tccctgactt gccgtcacgg
       61 aaggtcacgg cagtcccgag gctgtgcccg acttgaggcc ggctgcatca gcggggaacg
      121 attcgtctcg taggcgggcg ccatggcatg agcaccatct atctagattg cccgggcttt
      181 gacgttattg gcgattcgcg acgcataacc cgcttcggcc gaagtctcag gacagtcaag
      241 atcgggtgat ggatcgggaa gtcagtgccg gcgactccgg caacatcagc cactggccgc
      301 gcggcgaacg cccgcgggaa cgcctcatcg agcgcggcgc gcaggcgctg tcggatgccg
      361 aactgcttgc cgtggtcctc ggcaccggca gtggcggcgc caacgcgctc gacatcgcgc
      421 gccagctcct ccagcacttc ggcggcagcc tgcgccgcct gctcaccagc gacgctgaaa
      481 acctgctcgc gcttcggggc ctcggcccgg cgcgttacgc cacgctcatg gcctcgctcg
      541 agctcgcgcg ccgccactat tgcgaggccc tgcgtgcagg cccggcgctc aacgcgccgg
      601 cggccactcg gcagttcctg accgcgcaac tgcgagaccg gccctacgaa gtgttctgtt
      661 gcctgcatct cgacaaccgc caccggctca ttcacttcga agaggtgttc cgcggcacca
      721 tcgacggcgc cagcgtccat ccgcgcgaag tcgtgcgcca ggcactgcaa tacaacgccg
      781 cggcactcat cttcgctcac aaccacccgt ccggtgtggc ggaagccagc caggccgacg
      841 aactcatcac gcgccggctg cgcgacgcgc tcgcactcgt ggacatccgc gtgctcgacc
      901 acctcatcgt cggcgacaac aattgcctgt cgttcgcgga gcgcgggctg ctgtgactac
      961 ataaaactac tcagcctcag cccctcggcc tgctcatcgc cgtagcgtcc gggaagtcac
     1021 tcatgcgacg ctggcgacag agaacaagtg ccgggtcagt caaccggccg gaaggttcaa
     1081 cgatatgtcg tctatgtcgc gtggtttggt gtgggcagcg ctctgcgcga cgggtttgag
     1141 tgcgtgcagc ggaggcggtg gatcgaaacc gccgccgccg cccgctggcg ataccgtcgc
     1201 gcccgagact tccttcctgt cgactccgcc agcgctgacc aacgccacgg tcctggtttt
     1261 cggtctcacg tcgaatgaaa caggcgtcac cttcgaggtt ctcgccggcc tgccgaacta
     1321 cacgacgttc cccccgttgt tcggactcac cggtctcccg gatgggcccc acacgatctc
     1381 cgtgcgcgcg cgtgatgccg cggggaacgt cgacgcgact cccgcgacat acaacttcgt
     1441 catcgacacg caggcgcccg acaccacact cgcgacgtcg cccgcggcgg tgaccgcatc
     1501 gacgagcgcc acgttcacgt tcacttccga agccaacgcc acgttcgaag caagcgttga
     1561 cggtgcggcg ttcgcgaccg cggcgagccc gctcgcactc agcggtctcg ccaacggcgc
     1621 gcacaccgtc cagattcgcg cgcgcgatgc ggccaccaac gtggacgcct cacccgcctc
     1681 cttcagctgg caggtcgaca ccgccgcgcc cgcagtcagc atcgtgttcc cgacgcgtca
     1741 ttcgtacacg gaggcgaatt cgctgcacgt ccgcggcacc tcgagcgaca tctcgacgat
     1801 cacggcggtc agcgtgaacg gagtcgcggc aaccaccagc gatgcctatg cgcactgggc
     1861 agccgtggtg ccgattgcgc ccggcaacaa tgttctcaac gtgagcgtca cggattcact
     1921 gggcaatacc agcgccagcg cggcgcgcgc cgatgtcgcg aaccgcggcg tggcgatctc
     1981 caacatcgac agcgtcacgt gggatccggt ccgtggccgc gccatcgtcg ccgacaacga
     2041 ccgcggtgtg ctcatcgcga tcgacggcac caccggcaac gcgtccatca tctccggcgc
     2101 cgcgcgaggc acggggccgg cgctcggcgg cagttacttc ttcgcgaaga tggacacggc
     2161 taacgaccgg atattggtga tgcgtgggca gtcgctgatc gaggtggatc cggattccgg
     2221 cgatcgcgcg gaaatcccga ccgccgcgcc ggatgcgcac acgacattct tcggcagcac
     2281 catcgcctgc aacgccgatt gctcgctgct cttcctggcg cagatgtccg acccgttgaa
     2341 tggcggccag ggcatcttca gcatcagccc gcaaacgggc gcgcgcacgc tgatcaccgg
     2401 tggctggccg atcgtgggca gcggctacac gatccagacc gcgcgcggca tcgccttcga
     2461 tgcagtcaat tcgcgaatct atgtggtcga cggcagccgc aacggcctgt tcgcggtgaa
     2521 ccccgcgacg gcggatcgtg cactcatttc ttcatcgcag ccgccggcgt tcggcagcgg
     2581 cgtggacctg gtgaacccga gaggcatcgt cctcgacctc gccaacaacc gcgcgatcgt
     2641 caccgacacg ttgcgaaatc agcaggcgcg gctcgtcgcc atcgatcttt cgacgggcgc
     2701 ccgttccgtt ttcgcgacca ccatcgtgga cgcaacgctg gcccccgcga ccgacatgct
     2761 gctcgatgcc tcgggatcga gaatgctcat cggcatgaat cgcggaagca ttgcgcaggt
     2821 cagcctgacg tcgggcgatg tgcggcgttt ctcggacagc aacgtcggca cgggaatgag
     2881 cctgtggggt tggggcgcgc tgttgttcga gcctgccaac cgcactttgc tcaacgccgc
     2941 gaccggagcg atcgtgcgaa tcgatccggc gaccagcgat cgcagcgtcg tggttcccac
     3001 gctgctggcc agtggcaccg tgccgtatgc gccgtcgcat ctcaatgtcg acacgcgcgc
     3061 gggcgcgccg ccgaaccgcc tgatcggcgt cggccccggc agttcgccca cgatgttccg
     3121 cgcctatgac ctctccgccg gcacggacac ggaaatcgtc gcgacttcac ttccatacgg
     3181 ctcggcggtg tttccattcg acgcgccgaa tggacggatg at
//
LOCUS       DATDEU010000573         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2009135, whole genome shotgun sequence.
ACCESSION   DATDEU010000573 DATDEU010000000
VERSION     DATDEU010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009135"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1524
                     /locus_tag="VIV63_13680"
     CDS             154..1524
                     /locus_tag="VIV63_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125697.1"
                     /translation="MQPENVRSAADARKIVEERKLDHVKVGVFDNDGIMRGKYMGRDK
                     FFSALDKGFGFCDVVLGWDSNDQLYDNVKYTGWHTAYPDAPVRVLPDTCRVVPFEGDM
                     ILFLGEFAEKAEAVCPRATLRRVLKKADDLGYKVNAATEFEFFMFKESPESVRAKAFR
                     DLTTMTPGFFGYSVLRSSVHSEFYHQLMNMCTGLRIPLEGLHTETGPGVLEAAIQYCE
                     ALEAADRGALFKTYTKILAQRNGMMATFMAKWSKDWPGQSGHIHTSLTRKTDGKAAFH
                     DTSKPHNMSDEMRWFVGGQQKLMPELLAMVACTVNSYSRLIPGFWAPTTATWGVENRT
                     CALRVIPGGASSQRVEYRIAAADINPYLALAVAIGSGLYGIEHKIEPDAMVEGNAYEK
                     KFPKNRSLPRTLTEASEWLEKSKAGRSLFGDAFVEHFAASREWEDREFRRAITDWELA
                     RYFEII"
     gene            1521..>2288
                     /locus_tag="VIV63_13685"
     CDS             1521..>2288
                     /locus_tag="VIV63_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6125698.1"
                     /translation="MNQNGVQKTISPVDGRVYVERPLATSAQINETLRTARHAVAEWR
                     NTSIAERAAILTRFCAEFEKRGPQIAEELTWQMGRPARYAPNEVKGTLERARHMIAIA
                     PQALGDVATDPKDNFKRFVRREPLGVVFTVAAWNYPFLIAVNSVVPALMAGNVVVLKH
                     SAQTPLAAERFAECLTAAGLPEGAFQVLHASHEDTDIIIRDPRVDFVAFTGSVAGGRA
                     VQRAAAQRFIGVGLELGGCDPVYVRHDANLDHAIENIV"
BASE COUNT          426 a          761 c          718 g          383 t
ORIGIN      
        1 aagaatgggg tcagacccca ttttctagca aaaggggcca gagctctctt acacagacgg
       61 acgccggccc ttggccggcg ttttccgttg tggcgtttac gcctgacaag tctgacccca
      121 tactcctatg cactcatata agaagagtgc gccatgcagc cagaaaacgt ccgctcggcc
      181 gccgatgccc ggaagatcgt cgaggagcgc aagctcgatc acgtcaaggt cggcgtcttc
      241 gacaacgacg gcatcatgcg cggcaagtac atgggccgcg acaagttctt ctccgcgctc
      301 gacaagggct tcggcttctg cgacgtggtg ctgggctggg actcgaacga ccagctctac
      361 gacaacgtga agtacaccgg ctggcacacc gcctatccgg atgcgcccgt gcgcgtgctg
      421 cccgacacct gccgcgtcgt gcccttcgaa ggcgacatga tcctgttcct gggcgaattc
      481 gcggagaagg ccgaagcggt gtgtccgcgc gccacgctgc ggcgcgtact caagaaggcg
      541 gacgatctcg gctacaaggt gaacgccgcc accgagttcg aattcttcat gttcaaagaa
      601 tcgccggagt cggtgcgcgc caaggcgttc cgcgatctga ccaccatgac gccgggcttc
      661 ttcggctatt cggtgcttcg aagctccgtc cactccgagt tctatcacca gctcatgaac
      721 atgtgcacgg gcctgcgcat cccgctcgaa ggtctgcaca ccgagaccgg cccgggcgtg
      781 ctcgaagcgg ccatccagta ttgcgaggcg ctcgaagccg cggaccgcgg cgcgctgttc
      841 aagacctaca ccaagatcct cgcgcagcgt aacggcatga tggccacgtt catggccaag
      901 tggtcgaagg actggccggg gcagagcggg cacatccaca cctcgctcac gcgcaagacc
      961 gacggcaagg ccgcgttcca cgacacctcg aagccgcaca acatgtccga tgagatgcgc
     1021 tggttcgtgg gtggccagca gaagctcatg cccgagttgt tagccatggt cgcctgcacg
     1081 gtgaacagct actcgcgcct gatccccggc ttctgggcgc cgaccaccgc gacctggggc
     1141 gtcgagaacc gcacctgcgc gttgcgcgtg atccccggcg gcgcgtcgag ccagcgcgtg
     1201 gaataccgca tcgccgccgc ggacatcaat ccgtacctcg cgctcgccgt ggccatcggc
     1261 tccgggctgt acggcatcga gcacaagatc gagccggatg cgatggtcga gggcaatgcc
     1321 tacgagaaga agtttccgaa gaaccgttcg ctgccgcgca cgttgacgga ggcttccgag
     1381 tggctggaga aatccaaggc cggacgctcc ttgtttggcg atgcgttcgt cgagcacttc
     1441 gccgccagcc gcgagtggga agaccgcgag ttccgccgcg cgatcaccga ctgggagctc
     1501 gcgcgctact tcgagatcat atgaaccaaa acggcgtaca gaaaaccatc agccccgtcg
     1561 acgggcgcgt ctacgtcgag cgtccgctcg ccacgtcggc gcagatcaac gagactttgc
     1621 gcaccgcgcg tcacgcggtc gccgagtggc gcaacacctc catcgcggag cgggccgcga
     1681 tcctcacgcg gttttgcgcc gagttcgaga agcgcgggcc gcagattgcc gaggagctca
     1741 cgtggcaaat gggccggccg gcgcgttatg cgcccaatga agtgaaaggc acgctcgagc
     1801 gcgcgcggca catgatcgcc attgcgccac aggcgctcgg cgacgtggcc accgatccca
     1861 aggacaactt caagcgtttc gtccggcgcg aaccactcgg cgtggtgttc acggtcgccg
     1921 cctggaacta tccattcctc atcgcggtga acagcgtggt gcctgcgctc atggccggca
     1981 acgtggtggt gctcaagcat tccgcccaga cgccgctcgc cgccgaacga tttgccgagt
     2041 gcctgaccgc cgcggggctg cccgagggtg cgttccaggt gctgcacgcc tcgcacgagg
     2101 acaccgacat catcatccgc gatccgcgcg tggatttcgt cgcgttcacg ggctcagtcg
     2161 ccggcggccg cgccgtgcag cgcgccgcgg cacaacgctt catcggtgtg gggctcgaac
     2221 tgggtggctg cgacccggta tacgtgcgcc atgacgcgaa cctcgatcac gccatcgaga
     2281 acatcgtc
//
LOCUS       DATDEU010000574        11630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1863274, whole genome shotgun sequence.
ACCESSION   DATDEU010000574 DATDEU010000000
VERSION     DATDEU010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863274"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VIV63_13690"
     CDS             <1..691
                     /locus_tag="VIV63_13690"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY6125699.1"
                     /translation="GCRMVVQAQPFFAVPFGFAQYENCEKLNEELRALFLQREAEGAK
                     YANPRPFTQRNRQVYESNFQLFRSPEPCIQQLKNFCWSNLLGMIAELSKYDAATMRRM
                     LIFNDCWFHVTRRGGFFGLHNHPMASWSGVYCVDPGKHDADKPDSGMLTFVNPTTVSA
                     MYQDAATAALRGPFGSGIRSLKFEPGQLVLFPSYILHDVKPFEGEGERITVAFNCWFS
                     LKDDEPPSPPG"
     gene            complement(692..1069)
                     /locus_tag="VIV63_13695"
     CDS             complement(692..1069)
                     /locus_tag="VIV63_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125700.1"
                     /translation="MPRSLWFTLACALLVSSCASTPPDAAKPPAADAKPKPVWSVPTE
                     QETAAVLDKKFEEAAKSYVKLKKDGVLLFCKKRKEIGSNLPSIQCLTEAQLRNQVETM
                     DDYRERRRHAAKCTHGVGCTAGQ"
     gene            complement(1111..1518)
                     /locus_tag="VIV63_13700"
     CDS             complement(1111..1518)
                     /locus_tag="VIV63_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125701.1"
                     /translation="MTFAKNLKTLSILIAAAAFAGCASQPAVDPAPAKAPVAAAAPAS
                     TPATNAAVTEADTATSMEKRFQEAARSYKTVQKDGKTMYCKRERVIGTTIPTMQCLTE
                     AQLRNQVENMEEYRSRARNAAKCTHGPGCGAGG"
     gene            complement(1535..2014)
                     /locus_tag="VIV63_13705"
     CDS             complement(1535..2014)
                     /locus_tag="VIV63_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125702.1"
                     /translation="MIKTLNYALAALLLSSAPALPAEPLPPAIDACASLLRDSERLAC
                     FDKAVAALKTGAGTTMVTAENMFGANSDTSLASPDQKGVKREELKQIRGTVTSLRGIG
                     DGMIELELDNGQVWRQTDADVRMLVSTGDSVTIVRASLGTFRIADKTGRFARFRRVR"
     gene            complement(2011..3309)
                     /locus_tag="VIV63_13710"
     CDS             complement(2011..3309)
                     /locus_tag="VIV63_13710"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY6125703.1"
                     /translation="MSANDEALIRGLIQQAEKASAAGQRDESQRLLAQATGIAPEHPM
                     VLNARAIALLQSGDAASARTLLDKATAKDSANPALWMNLATALRGLDLRDEEEKALQS
                     VLTIEPRHLLALLQKAELLERRGKTKAAAKAYQNALQTIAPGVRLPVALKNLIAKAVD
                     AVRRNDAALAAHLGASAEPVRARHSPAELDRAEHGIAAFLGTRRIYQSQPTFLHVPKL
                     ANLEFFPREDFPWLDEFEAATAEIRAECERVLREDDSELVPYIDYPDGMPIDQWAELN
                     KSRRWSAFFLWRDGKRIDEHADRCPRTAALLAKAPVSDVPGYSPTAFFSILDRNARIP
                     PHMGVTNSRLIVHLPLVVPPRCGFRVGSETREWQEGKAWVFDDTIEHEAWNESDVPRA
                     ILIFDTWHPALNDGERALIRTTVPAIKDYYRDEIEISGSE"
     gene            complement(3328..4620)
                     /locus_tag="VIV63_13715"
     CDS             complement(3328..4620)
                     /locus_tag="VIV63_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6125704.1"
                     /translation="MRRITSLALAAISTVFTAALTTGTAYAGAADDSWYIAPQVNALW
                     LDDGRVADDDAGVTIAFGRTFSPKWDAQVALYGSEHQRTGDNSLELQGLALSLNRVYY
                     REGRVNPFLSFGVARNKSILKSGVDEENLYVLYGAGLLITLGKERDDGSAMQLRADLG
                     ARHGLSDEDGAGVEKPVDYVAGIGIQYSWGGSTRSLDSDGDGVNDDTDQCPGTPAGTA
                     VDSTGCPLPQDDDGDGVTNDIDKCPGTPAGARVDATGCELDSDGDGVSDSRDQCPNTP
                     PGAKVNESGCELDTDGDGVIDRLDKCPDTPKGDRVDAVGCSFKEEIKLPGVVFETGKA
                     DLKPESIPVLEGAIATLKRYPDINIEVAGHTDSRGSDALNLDLSSRRADTVLKYLQDG
                     GVTNAITARGYGERQPVASNSNDDGRQQNRRVVLRVLN"
     gene            complement(4666..5187)
                     /locus_tag="VIV63_13720"
     CDS             complement(4666..5187)
                     /locus_tag="VIV63_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY6125705.1"
                     /translation="MTAMRPNPRRPDLLRRLATVFAATLALAGSTMVSAQEGQLEELA
                     AFPRGKLEINDGKKLKLTFDVWLADNPRRQAQGLMFVRALPDMRGMLFVHENPKPMSM
                     WMKNTYIPLDMVFIDAQSRIQQIVEQTTPHSLAIISSKDPALAVLEIAGGEAKRLGLH
                     PGQRVVHPALVKR"
     gene            complement(5153..5599)
                     /locus_tag="VIV63_13725"
     CDS             complement(5153..5599)
                     /locus_tag="VIV63_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125706.1"
                     /translation="MDLDLDWKDVQMQCEGGPRPSGNGIRVSIGGPERGDERRIRLVF
                     GIAGVGEGRGGQTLRTNVTILFEGERRVFATLGDDKCTVDSLTQQRVESLGGNRAIYR
                     VEARGFCLGPATSLSKGERMLLTSFDFAGRVEFNDDDRHAPKPPTP"
     gene            5814..7079
                     /gene="glgC"
                     /locus_tag="VIV63_13730"
     CDS             5814..7079
                     /gene="glgC"
                     /locus_tag="VIV63_13730"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY6125707.1"
                     /translation="MAKRPARATSGRYVSRLTSGTLAVIMAGGRGERLKGLTEHRAKP
                     ATPFGGKFRIIDFVLSNCVNSGIRQITVLTQYKAQSLIQHIHRGWGYMRGEFGEFVEV
                     IPAQQKTGNHWYRGTADAVYQNIDVMHSHRPKHVLVLAGDHIYKMDYGPMIAYHVEKG
                     ADITVGVVEVPIVEAKEFGVLTVTEWNRVTRFVEKSPTPDPMPGRTDVALASMGIYVF
                     NPDLLEKLLLEDAADEKSKHDFGKDIIPKAIEKLQVFAYPFRDVKTRAQSYWRDVGTI
                     DAFYEANLELVWVAPELNIYDEDWPIWTYQAQTPPAKFVLDEDGRRGVAINSLVSGGA
                     IISGATVRESLLFSGVRVEERSLVERSVILPDVRIGQNCEVRNAIIDAGCEIASGTRI
                     GIDRAEDARRFLVTEKGVVLVTQDMLSHV"
     gene            7108..7551
                     /locus_tag="VIV63_13735"
     CDS             7108..7551
                     /locus_tag="VIV63_13735"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEY6125708.1"
                     /translation="MALITLPLVSLFGGCAAIPKLEAPKLSVVSLKTAGGDIFSQRVV
                     VRMRVFNPNTRELPIQGLTYTIEVNDAELGNGGTAEPFVVPAMGEAEFDMQITANLAG
                     ALAKLLLTRRSSSESLDYRLRGSVNLSSGFLRRIPFDERGTVKLK"
     gene            complement(7489..9009)
                     /locus_tag="VIV63_13740"
     CDS             complement(7489..9009)
                     /locus_tag="VIV63_13740"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY6125709.1"
                     /translation="MPAAVKLHICAIASEVVPLAKSGGLADVSSALSRQLTVAGHDVR
                     LFMPFYSQIKRDKLGATRVDGLQGLVFAIGPREYRVDIWRANLPGSASPVYLVENDAL
                     FARPKLYTGDPDEHRRFLVLTRAALESCRHLNFRPHIVHSHDWHTAFAPLWLKSIYKN
                     DPVFAAAKSVMTIHNIGYQGEFDAIDVADLDLGADAYLLHQDDLKAGRVNALKHGILH
                     ADAVTTVSPTYAREITTPRYGMGMEQALASRGNAVHGILNGVDYEEWDPARDKYLPIH
                     FGPGDVTQKAELKRNFLLRQGMVPTRGRVPLFGIVSRLASQKGFDLLTDTLPKLLQTN
                     DVRLAVVGTGDPVYEKFFTGLAQKYRGRAWFFGGYDDSLAHWIEGASDVFLMPSQYEP
                     CGLNQMYSLRYGTIPIVRKTGGLADSVTHFDPAMGVGTGIVFNDFDAAAMTWALDTAM
                     GWYLQPALWQRIVENALRCDFSWETQANEYLKLFAELLQLDGAALVKRNPAQKTAG"
     gene            9060..10235
                     /locus_tag="VIV63_13745"
     CDS             9060..10235
                     /locus_tag="VIV63_13745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6125710.1"
                     /translation="MRRILTFLTAIVLLLVSLGVGVFTADLPFWRRAFQLPLAPGEAY
                     LPVAVIGASGSAPAPGANAAGPAIDALVVEEAANRAGAAGSRALLVMNRGTIVVERYF
                     LTDDASTLLPASLVARPIAAMAIGLAIADGRIAGIDTPVARYLTEWDDEPRGRITLRQ
                     LLEETSGLETGGDVRGLLYRSPWSDLKSLPAFATSRGVRMLLGNDFESSALGFRLDHE
                     PGGFYNVSPANTQLAAVIIERATDEPYETFVDRKVWQPVGAGTAELQLDRRAGMPAAH
                     CCWRATARDMLRVLNLLGTGGKHADHPVLPEEWVREMARPSRVSAGTGMQVTRITIEG
                     EEALMVTDDTGSAAWILPRRELTILNITNPGGSAVADLPAMLLKGFEGPAPVLQTAQ"
     gene            10289..11155
                     /locus_tag="VIV63_13750"
     CDS             10289..11155
                     /locus_tag="VIV63_13750"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY6125711.1"
                     /translation="MDMKVTSISEGFLDSREFARMTRAIEFIEREYQRQPKLAQIAKV
                     VGISEFHFNRLFRRWTGLTPKQYLAEVTSRAAGAALRDEPSVLDAAHSVGLSGGGRLH
                     DLTVSLEAMTPGEIRTGGAGVNIRHGVAETPFGTAFVAETERGLCRLAFLDGASDATE
                     LKALRSQWPEAVYTRDDDRAKTLVETIWGHKSAKLPLAVCGTNFQVQVWRALLELDPG
                     ETVTYSALARHVGQPNAARAVGNAVGANPIAWVIPCHRVLRTGGKLGGYRWGVARKQM
                     IRRWEERRTGVS"
     gene            11237..>11630
                     /locus_tag="VIV63_13755"
     CDS             11237..>11630
                     /locus_tag="VIV63_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY6125712.1"
                     /translation="MSEKRSLATPISIAIAVVCAAWAIYATQHRPTAAAGGPPSGGMR
                     NNAPAAVMLAPVRAERISQKLEALGNARANESVDISTKISNIVTAVTFRDGERVRRGQ
                     VLVQLDNAQARADVAAAQAAVTESESLYN"
BASE COUNT         2017 a         3697 c         3756 g         2160 t
ORIGIN      
        1 gggttgcagg atggtcgtac aggctcaacc ctttttcgca gtgcccttcg gcttcgcgca
       61 atacgagaat tgcgagaagc tcaatgagga actgcgcgcg ttattcctgc agcgcgaggc
      121 cgaaggcgcg aaatacgcga atccgcggcc cttcacgcag cgcaatcgcc aggtctacga
      181 gagcaatttt cagctgttcc gctcgccgga accctgcatc cagcaactca agaatttctg
      241 ttggagcaat ctgctgggga tgatcgcgga gctcagcaag tacgacgctg ccaccatgcg
      301 tcgcatgctc atcttcaatg actgctggtt ccatgtgacg cggcgtggag gcttcttcgg
      361 actgcacaac catccgatgg ccagttggtc gggcgtttac tgcgtcgatc ccggcaaaca
      421 cgacgcggac aagcccgaca gcgggatgct gaccttcgtg aatccaacga cagtcagcgc
      481 gatgtaccag gacgcggcca ccgcggcgtt gcgcgggccg ttcggttcgg gaattcgttc
      541 gctgaaattc gagccgggtc agctcgtgct gtttccgtcg tacatcctgc acgacgtaaa
      601 accattcgaa ggcgagggtg aacgaatcac cgtcgccttc aattgctggt tttctctcaa
      661 ggacgacgag ccgccgtcac cgccgggctg actactggcc ggccgtgcaa cccacgccat
      721 gcgtgcactt ggccgcatgg cgccgccgct cgcggtagtc gtccatggtc tctacctggt
      781 tgcgcagctg cgcctcggtg agacactgga tcgacggcag gttgctgccg atttccttgc
      841 gtttcttgca gaagagcagc acgccgtctt tcttgagctt cacgtagctc ttcgcggctt
      901 cttcgaactt cttgtcgagc accgcagccg tttcctgctc ggtgggtacc gaccagaccg
      961 gcttcggctt ggcgtcggcc gcggggggct tcgccgcatc cggcggtgtc gatgcgcagc
     1021 tcgaaacgag gagcgcgcag gcgagcgtga accacaatga tctgggcatc gcgagtcctc
     1081 ccatacgttg gcggcccagg gccgccgcaa tcatccgcct gcgccgcaac ctggtccgtg
     1141 cgtgcacttc gcggcgttac gcgcgcggct gcggtactcc tccatgtttt ccacctgatt
     1201 gcgaagctgt gcttcggtca gacattgcat ggtgggaata gtcgtgccga tgacgcgttc
     1261 gcgcttgcag tacatggttt tgccgtcctt ctgcacggtc ttgtagctgc gcgctgcttc
     1321 ctgaaatcgc ttttccatcg acgtggcggt gtccgcttcg gtgaccgccg cgttagtcgc
     1381 tggcgtgctc gccggggccg cggcggccac gggtgccttg gcgggcgccg gatcgacagc
     1441 gggctgcgac gcacatcccg cgaaggcggc agcggcaatc aggatggaga gagtcttaag
     1501 gtttttcgca aatgtcattc ttcactacct cggatcaacg cacgcgtctg aaacgcgcaa
     1561 aacgaccagt cttgtcggcg atgcggaacg tacccaacga cgcccggaca atggtcactg
     1621 aatcacccgt cgacaccagc atccgtacgt cggcatcggt ctgccgccac acctgcccgt
     1681 tatcaagttc gagctcgatc atcccatcgc caatgccccg cagggaagtg acggttccac
     1741 ggatctgctt gagttcttcc cgcttgacac ccttctggtc ggggcttgcc aggctcgtat
     1801 cgctgttggc accgaacata ttctccgcgg tgaccatcgt cgttcccgcg ccggttttca
     1861 gggcggctac cgccttgtcg aagcaggcca ggcgctcgga atcccgcagc agggatgcac
     1921 aggcgtcgat ggcgggcggg aggggttcgg ccggcagtgc cggcgccgag ctcaggagga
     1981 gggctgcaag ggcgtaattg agggtcttga tcattcgctg cctgaaattt cgatctcgtc
     2041 gcgatagtaa tctttgatag ccggcaccgt ggttcggatc agcgcgcgtt cgccatcgtt
     2101 gagcgcaggg tgccaggtat cgaatatgag tatggccctg ggtacgtcac tttcgttcca
     2161 tgcttcgtgt tcgattgtgt cgtcgaacac ccaggccttt ccttcctgcc actcgcgggt
     2221 ttccgatccg acgcgaaagc cgcaacgcgg cggcacgacc agcggcaggt gcacgataag
     2281 ccgcgaattc gtcactccca tgtgcggtgg aatgcgtgcg ttccggtcga ggatcgagaa
     2341 gaaggccgtc ggcgaatagc cggggacgtc agacaccggc gccttggcca gcagggccgc
     2401 ggtccggggg cagcggtccg cgtgctcgtc gatacgctta ccgtcacgcc acaggaaaaa
     2461 cgcgctccat cgccgcgact tgttgagctc cgcccattga tcgatgggca tgccatccgg
     2521 gtagtcgatg taagggacca gttcgctgtc gtcctcgcgc aatacccgtt cgcattcggc
     2581 gcgaatctcg gcagtcgccg cttcgaactc atcgagccac ggaaaatcct cgcgcgggaa
     2641 gaactcgaga ttcgcaagct tgggtacgtg gagaaatgtg ggctgcgatt gatagatgcg
     2701 ccgcgtgccg aggaatgccg cgatgccgtg ctccgcccgg tccagttcgg cgggagagtg
     2761 ccgggcacgt acgggctcgg cgctcgcgcc gagatgcgcg gccagcgccg cgtcgttgcg
     2821 gcgaacggcg tctacggcct tcgcgatcag gttcttgagc gccacgggca atcgcacgcc
     2881 cggcgcaatc gtctgcagcg cgttctggta ggccttcgcc gcggccttcg tcttgccacg
     2941 gcgctcgagc aactccgcct tctgaagcag cgcgagcagg tgacggggtt ctatcgtcag
     3001 taccgactgc agcgctttct cttcctcgtc ccgcaggtcg agcccacgca gcgcggtcgc
     3061 gagattcatc cacagcgcag ggttggcgga atccttcgcc gtcgccttgt cgagaagcgt
     3121 acgtgcactg gcggcatcgc ctgactggag cagggcgatg gcgcgcgcat tgagcaccat
     3181 ggggtgctcg ggcgcgatcc ccgtcgcctg cgcgagcagc cgctgcgatt catcacgctg
     3241 acccgcggcg gaagcctttt ccgcctgctg gatcaatcca cggatcagcg cttcatcgtt
     3301 cgcggacaat ctcgttcttt ggccggatca gttcaggact cgcaacacga cgcgccggtt
     3361 ttgctgccgg ccgtcatcgt tgctgttgct cgctaccggt tgacgctcgc cgtatccgcg
     3421 cgcggtgatg gcattggtca cgccaccgtc ctgcaggtac ttgagaaccg tgtcggcgcg
     3481 gcgcgaagac agatccaggt tgagcgcatc cgaaccgcgg ctgtcggtgt gaccggcgac
     3541 ctcgatgttg atgtcgggat accgcttgag cgtcgcgatg gcgccttcca gcaccgggat
     3601 gctttccggc ttgagatcgg ccttgccggt ctcgaacacg acaccgggta gtttgatctc
     3661 ttccttgaac gagcagccga cggcgtccac gcgatcgccc ttcggcgtat ccgggcactt
     3721 gtcgaggcgg tcgatgacgc cgtcaccgtc ggtgtcgagt tcgcagccgc tctcgttcac
     3781 cttggcgccg ggcggagtgt tcgggcactg gtcacggctg tcgctgacgc cgtcaccatc
     3841 gctgtcgagt tcacaaccgg tggcatccac cctggcgccc gccggagttc cggggcactt
     3901 gtcgatgtcg ttcgtgacac cgtcaccatc gtcgtcctgc ggcagtgggc aacccgtgga
     3961 atccaccgcg gtcccggccg gggtgccggg gcactggtcg gtgtcgtcat tgacgccatc
     4021 gccgtcggaa tcgaggctgc gagtcgagcc accccaggaa tactggatgc cgatgcccgc
     4081 gacgtagtcg acgggttttt cgacgccggc gccgtcttca tccgacaggc cgtgtcgcgc
     4141 accgaggtcc gcgcgcagct gcatggcgct gccatcgtcg cgctccttgc cgagcgtgat
     4201 gagcaggccc gcgccgtaca acacgtacag attttcttcg tcgacacccg acttcaggat
     4261 ggacttgttg cgcgccacgc caaaactcag gaagggattc acgcggcctt cgcggtagta
     4321 cacgcggttc agcgagaggg cgagaccttg cagctccagc gagttgtcgc cggtgcgctg
     4381 gtgttcggac ccgtagagcg cgacctgcgc gtcccacttc gggctgaagg tgcggccaaa
     4441 cgcgatcgtc acgccggcgt cgtcgtccgc gacgcggccg tcgtccagcc agagcgcatt
     4501 tacctgcggc gcgatgtacc agctgtcgtc ggcagcgccg gcgtacgccg tgcccgtcgt
     4561 cagagccgct gtaaacaccg tcgaaatggc cgcgagcgcg aggcttgtga ttcttctcat
     4621 ggttttctcc ctgaaaacag gtcctagcaa gtggcggcga tgatatcagc gcttgacgag
     4681 cgccggatgc accacgcgct ggccggggtg aaggccgagg cgcttcgcct caccaccggc
     4741 gatttccagc accgcgagcg ccgggtcctt gctgctgatg atggcgaggg aatgcggcgt
     4801 ggtctgctcg acgatctgct ggatgcggga ctgtgcgtcg atgaacacca tgtcgagcgg
     4861 gatgtaggtg ttcttcatcc acatgctcat cggcttaggg ttttcgtgga caaacagcat
     4921 gccgcgcatg tcgggcagcg ccctgacgaa catgagcccc tgcgcctggc ggcgcggatt
     4981 gtcggccagc cagacatcga aggtgagttt cagcttcttg ccgtcgttta tctcgagctt
     5041 gccgcgcggg aatgcggcca gctcctcgag ctgcccctcc tgggcggaaa ccatcgtgct
     5101 gcccgccagg gcgagcgtgg cggcgaacac cgttgccagg cggcgcagga ggtcagggcg
     5161 tcgggggttt gggcgcatgg cggtcatcgt cgttgaattc cacgcggcct gcaaagtcga
     5221 agctggtgag cagcatccgt tcccctttcg agaggctggt ggcgggtccg aggcagaatc
     5281 cgcgcgcttc gacccgatag atagcgcggt tgccccccag cgattcgacg cgctgctggg
     5341 tcagcgaatc cacggtgcac ttgtcgtcac ccagggtcgc gaaaacgcgg cgctcgcctt
     5401 cgaacagaat ggtgacgttg gtgcgcagag tctggccgcc tcggccttcg cccacgccgg
     5461 cgataccgaa gaccagccgg atgcgccgct catcgccgcg ttccgggccg ccgatgctca
     5521 cgcgaatgcc gttgcccgaa ggcctggggc cgccttcaca ctgcatctgc acgtctttcc
     5581 agtcgaggtc caggtccacc gcgccgcgca gacgcgcccg caggtagccg tcgcccgacg
     5641 ccaggcagcc ggtcgcggga gcgggcgcgg cggctgattt tgtaggagga gcggcgcacg
     5701 cggagtgcag gagcgctacg ccaagcgcag caacacaggc tgcagcggat gcgcgatgag
     5761 gcatgcgtct acggtagcac gggccctata cgtcaaaaaa accgagggac ccaatggcca
     5821 aacgcccagc acgcgcaact tccggtcgtt acgtcagccg gctcaccagc ggcacgctcg
     5881 ccgtcatcat ggcgggtggc cggggcgaac gcctcaaggg cctcacggaa caccgcgcga
     5941 aacccgcgac gccgttcggt ggcaagttcc gcatcatcga cttcgtcctg tccaactgcg
     6001 tgaattccgg catccgccag atcaccgtgc tcacgcagta caaggcgcaa tcgctgatcc
     6061 agcacatcca tcgcggctgg ggctacatgc gcggcgagtt cggcgaattc gtcgaggtca
     6121 tccccgcgca gcagaagacc ggaaaccact ggtatcgcgg caccgcggac gcggtctacc
     6181 agaacatcga cgtgatgcat tcgcatcgcc ccaagcacgt gctggtcctg gcgggcgatc
     6241 acatctacaa gatggattac ggcccgatga ttgcctacca cgtggagaag ggcgccgaca
     6301 tcaccgtggg cgtggtggaa gtccccatcg tcgaggcgaa ggaattcggc gtcctcacgg
     6361 tcaccgaatg gaaccgcgtg acgcgcttcg tcgagaagtc accgacgccg gacccgatgc
     6421 cgggccgcac cgacgtcgcg ctggcctcga tgggcatcta cgtcttcaac ccggacctgc
     6481 tcgagaagct gctgctcgaa gacgccgcgg acgagaagtc gaaacacgat ttcggcaagg
     6541 acatcattcc caaggccatc gagaagctgc aggtctttgc gtatcctttc cgggacgtta
     6601 agacgcgtgc acagtcgtat tggcgcgatg ttggaacaat cgacgccttt tacgaggcca
     6661 acctggagct cgtctgggtc gcgcccgaac tcaatatcta tgacgaggac tggcccatct
     6721 ggacctacca ggcacagacc ccgccggcca agttcgtgct cgatgaagac ggccgccggg
     6781 gcgtggcgat caactcgctg gtttcgggcg gcgccatcat ctccggcgcc accgtgcgcg
     6841 agtccctgct gttctcgggc gtgcgtgtcg aagagcgttc gctggtcgaa cgctcggtga
     6901 tcctgccgga cgttcgcatc ggccagaact gcgaagtgcg caacgccatc atcgacgcgg
     6961 gctgcgagat cgcgagcggc acgcgtatcg gcatcgaccg cgccgaagac gccaggcgat
     7021 tcctggtgac cgagaagggc gtggtgctgg tgacgcagga catgctctcg catgtgtgag
     7081 caaaaccggg tccggcgctc aggcttgatg gcgctgatca cgctgccgct cgtttcgctg
     7141 ttcggcggct gcgcggcaat tccgaaactc gaggcgccga agctgtcggt ggtgtcgctc
     7201 aagacggccg gcggcgatat cttttctcag cgcgtggtgg tccgcatgcg tgtgttcaat
     7261 ccgaacacgc gcgaacttcc gatccagggc ctcacctaca ccatcgaggt caacgacgcc
     7321 gaactcggca atggtggcac cgcggagcca tttgtcgtgc ccgccatggg cgaggcggag
     7381 ttcgacatgc agatcacggc gaatctcgcc ggcgcgctcg cgaagctgtt gctgacccgg
     7441 cgcagttctt cggagtcgct cgactaccgc ctgcgcggaa gcgtcaatct atccagcggt
     7501 tttctgcgcc ggattccgtt tgacgagcgc ggcaccgtca agctgaagta gctccgcgaa
     7561 caatttcaga tattcgttcg cctgggtttc ccaggaaaaa tcgcagcgca gggcgttttc
     7621 gacgatgcgc tgccagagcg ccggctgcag gtaccagccc atcgccgtat cgagcgccca
     7681 ggtcatggcc gccgcgtcga agtcgttgaa cacgatgccg gtgccgacac ccatggccgg
     7741 atcgaagtgc gtgaccgaat cggcgaggcc gccggtcttg cgcacgatcg gaatggtgcc
     7801 gtaacgcagc gaatacatct gattgaggcc gcagggttcg tactgcgaag gcatcaggaa
     7861 cacatcgctc gcgccttcga tccagtgagc cagcgagtcg tcataaccgc cgaagaacca
     7921 cgcgcggccg cggtatttct gcgcgagccc ggtgaagaac ttttcataca ccggatcgcc
     7981 ggtgccgacg accgcgagac gcacgtcgtt cgtctgcagc agcttcggca gcgtgtcggt
     8041 gagcaggtcg aaacctttct gcgaagcgag ccggctcacg atgccgaaca gcggcacgcg
     8101 tccgcgcgtc ggcaccatgc cctggcgaag caggaaattg cgcttgagct cggccttttg
     8161 cgtcacatcg ccgggaccga aatggatcgg cagatatttg tcgcgcgccg gatcccattc
     8221 ctcgtagtcg acgccattga ggatgccgtg caccgcgttg cctcgcgaag ccagcgcctg
     8281 ctccataccc atgccgtaac gcggcgtggt gatttcgcgc gcataggtcg gactcacggt
     8341 ggtcaccgcg tcggcatgca ggatgccgtg tttgagcgcg ttgacgcgcc cggccttgag
     8401 gtcgtcctgg tgcagtagat aggcgtccgc gccgagatcg aggtcggcga cgtcgatggc
     8461 gtcgaactcg ccctggtagc cgatgttgtg gatggtcatg accgacttcg ccgcggcgaa
     8521 caccgggtcg ttcttgtata tggacttgag ccacagcggc gcgaaggccg tgtgccagtc
     8581 gtgcgaatgg acgatgtgcg gccggaaatt caggtgccgg cagctttcga gcgcggcgcg
     8641 cgtcagcacg aggaaacggc gatgttcgtc gggatcgccg gtgtagagct tcgggcgcgc
     8701 aaacaacgcg tcgttctcca ccaggtacac cggagaggcc gatcccggga gattcgcgcg
     8761 ccagatgtcg acccggtatt cgcgggggcc gatggcgaac accaggccct ggaggccgtc
     8821 gacacgggtg gcgccgagtt tgtcccgctt gatctgcgaa tagaacggca tgaacagccg
     8881 cacgtcgtgc ccggcgaccg tcaattgccg cgaaagcgcg ctcgagacgt cggcgaggcc
     8941 gccgctcttg gcgagcggga ccacttcgga tgcgatggcg cagatatgta gtttcacggc
     9001 ggcgggcatt ttagcctcat ggtcacgctg gtagcatgtc gcaaggtctt aataattcca
     9061 tgcgccgaat actcactttc ctcacggcca tcgtgctgct gctggtgtct ctcggcgtcg
     9121 gcgtgttcac ggccgacctg ccgttctggc gtcgcgcctt ccagttgcca ctggcccccg
     9181 gggaagccta cctacccgtg gcggtcatcg gcgcttccgg ctctgcgccc gcgccaggcg
     9241 cgaatgccgc ggggccggcg atcgacgcgc tggtggtcga ggaagccgcg aaccgcgcag
     9301 gcgccgccgg ctcccgcgcc ttgttagtca tgaatcgcgg cacgatcgtc gtcgagcgct
     9361 atttcctgac cgacgacgcg agcaccctgc tgcccgcatc gctggtggcg cgtccgattg
     9421 cggccatggc catcggcctg gcaatcgccg acggccgtat cgccggcatc gacacgccgg
     9481 tcgcgcgtta cctcaccgag tgggacgacg agccacgagg ccgcatcacg ttgcgccagc
     9541 ttctcgaaga aaccagcgga ctcgagaccg gcggcgacgt tcgcggtctg ctgtatcgct
     9601 cgccgtggag cgacctgaaa tcgctgccgg ccttcgccac gtcgcgcggc gtccgcatgt
     9661 tgttaggcaa cgatttcgaa tcgagcgcgc tcggcttcag gctcgatcac gagccgggtg
     9721 gtttctacaa cgtctcgccg gccaacacgc agctcgccgc ggtcatcatc gaacgtgcca
     9781 cggacgagcc gtatgaaact ttcgtggacc gcaaagtgtg gcaaccagtc ggcgccggga
     9841 ccgccgagct ccagctcgat cgccgcgccg gcatgcctgc cgcgcattgc tgctggcgcg
     9901 cgaccgcgcg cgacatgctg cgcgtgctga acctgctggg aacgggcgga aaacacgcgg
     9961 accacccggt gctgccagaa gagtgggtgc gcgagatggc gcggccttcg cgcgtgagcg
    10021 ccggcaccgg catgcaggtg acgcgtatca ccatcgaagg tgaggaggcc ctcatggtca
    10081 ctgacgacac cggaagtgcg gcctggatcc tgccccggcg tgagctcacc attctcaaca
    10141 tcacgaaccc tggcgggagc gcggtcgccg atctcccggc catgttgctg aagggcttcg
    10201 aagggcccgc gccagtcctg caaacggcgc agtgaaacgc gcaaggatcg ggtcgaaagc
    10261 ccgaaggccg gcgcccagga tgcggtccat ggacatgaaa gtaacctcga tatcggaagg
    10321 atttctcgac agccgggaat tcgcgcgcat gacccgtgcg atcgaattca tcgaacgcga
    10381 gtatcagcgc cagccgaaac tcgcgcagat cgccaaggtc gtcgggatat ccgaattcca
    10441 cttcaaccgt ctgttccgtc gctggaccgg actgaccccc aagcagtacc tcgcggaagt
    10501 caccagtcgg gccgcgggcg cggcgttgcg cgacgagcct tcggtgctcg acgccgctca
    10561 ctctgtagga ctttcgggcg gcggccgatt gcacgatctg accgtgtcgc tcgaggccat
    10621 gacacctggc gaaatccgca ccgggggtgc gggcgtgaat attcgtcatg gagtagcgga
    10681 gacgcctttc ggtacggcgt tcgtcgccga gacagagcgc ggtttatgtc ggctggcgtt
    10741 cctcgatggt gcaagcgatg cgacagaact gaaagcgctg cgttcgcagt ggccggaggc
    10801 cgtttacacg cgcgatgacg atcgggctaa gactttggtc gaaaccatct ggggtcataa
    10861 atccgcaaag ttgcctctgg cggtgtgcgg aacgaacttt caggtgcaag tctggcgtgc
    10921 gttgctcgaa ctagatcccg gcgagacggt cacatacagc gcgttagcac ggcacgtggg
    10981 tcagcccaat gctgcccggg ccgtcggtaa cgcagttggc gcgaatccca tcgcctgggt
    11041 catcccctgt catcgcgtgc tgcgaacggg cggaaagctc ggcggatacc gatggggagt
    11101 cgcgcgcaaa cagatgattc gccgctggga agaaaggcgt accggggtct cataacgttt
    11161 tgattaccct cgtgtgtcga cgtgttagcg gtgccatact tgtcctccgc caacgaacga
    11221 ttcaacgccg cacacgatgt cagagaagcg cagcctcgcc acacccatca gcattgccat
    11281 cgcggtcgtt tgcgccgcct gggctatcta tgcgacgcag cacaggccca cggctgcggc
    11341 gggcggcccg ccgtccggtg gcatgcgcaa caacgcaccc gcggcagtca tgcttgcgcc
    11401 ggtacgcgcc gaacgtatca gccagaagct cgaggcgctg ggcaacgcgc gcgccaatga
    11461 atccgtcgac atcagcacca agatttcgaa catcgttacg gcggtgacgt ttcgcgatgg
    11521 cgagcgcgtc aggcgcggcc aggtgctggt gcagctcgac aatgcgcagg cgcgtgccga
    11581 tgtggccgcc gcgcaagccg cggtaaccga aagcgagagc ctctacaacc
//
LOCUS       DATDEU010000575         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_258062, whole genome shotgun sequence.
ACCESSION   DATDEU010000575 DATDEU010000000
VERSION     DATDEU010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258062"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VIV63_13760"
     CDS             <1..495
                     /locus_tag="VIV63_13760"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEY6125713.1"
                     /translation="QTEALAQGHEGIMAKLLDSPYEAGNRGASWLKIKRAHTLDLVVL
                     GAEWGHGRRHGKLSNLHLGAFDPATQQYVMLGKTFKGLTDAMLEWQTREFLAREASRD
                     ASTVYVRPEIVAEIAFSDLQSSPRYAGGLALRLARVKQYRLDKRVEDADTMETVRKLF
                     AAQG"
     gene            complement(588..1355)
                     /locus_tag="VIV63_13765"
     CDS             complement(588..1355)
                     /locus_tag="VIV63_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125714.1"
                     /translation="MTTSNESAVSWGAVIAGAVGAAALSLVLFVLGTGLGLAVVSPWE
                     SEGISGKAAGVSTIVWVTVVQLLASVLGGYLAGRLRTRWVTVHTNEVYFRDTAHGFLA
                     WAVATLLMATLLSSAVASAVSTGLKAGDSSPVGYSIDSLFRGGAPDNTAVSPDRTEEV
                     TRILANGLQSGELSDEDRQYLGQVVAANTGLAQSAAEQRVGETFVKLKKGADDARKAS
                     AGLALWLVVSLLVGAFVASFAATLGGRLRDSSVLAAS"
     gene            complement(1414..1872)
                     /locus_tag="VIV63_13770"
     CDS             complement(1414..1872)
                     /locus_tag="VIV63_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6125715.1"
                     /translation="MSMRLIPRTRDEVRATLQVLPPEVRAQISPNWLALLEKSAPQDP
                     WVHGFHVVDDAGVTVGLGAFKGPPVEGMVEIAYAIEPEHQGKGHATAAARALAEFALA
                     SDQVCVVRAHTLPDGIASQRVLLKSGFEKMGEVIDPEDGLVWRFELVRRG"
     gene            complement(1869..>3049)
                     /locus_tag="VIV63_13775"
     CDS             complement(1869..>3049)
                     /locus_tag="VIV63_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125716.1"
                     /translation="LLFVLVLSSLAWLPAHAQGHYLFAWTGDEKKEGNDFLAVIDANP
                     NSTGYGKLVASVGTDQKTVRIHHTEYVMPASGMLFANDHDVGRTFIFDVNDPLHPRVA
                     ASFGDMAGYTHPHSFVRLPNGNVIATFQHAHHGHHAGELGATGGLVEIDDKGKVVRSV
                     STADPAFPDALLMPYGVAVLPEQDRIVSTNSSMHDPDLLSGVTYQVWRLSDLKLLETA
                     YFDPGEGRYAHIAPEEPRVAADGSVYVQTLGCGIERITGLTSKPVAKLVHTFPGNWCG
                     VPTIVGNFLIQSVPAINGLIALDISNPQKPKEVSRLTLSDGFDAHWTGWDAKTKRLVT
                     TGSENRLFLVKLDAKTGALSVDEAFKDDNGKPGFDLANRKWPHGWTGTGKPHGVVFSR
                     "
BASE COUNT          593 a          987 c          948 g          521 t
ORIGIN      
        1 cagaccgaag cactcgcgca ggggcacgag ggcatcatgg ccaaactact ggattcgccc
       61 tacgaagcgg gcaaccgggg cgcgagctgg ctcaagatca agcgtgcgca cacgctcgac
      121 ctcgtcgtgc ttggcgcgga atggggccat gggcgccggc acggaaagct ctccaacctt
      181 catctcggcg cattcgaccc ggccacgcag caatacgtga tgttaggcaa gacgttcaag
      241 ggcctgacgg acgccatgct cgaatggcag acccgcgaat tcctggcccg cgaagcaagt
      301 cgggatgcgt cgactgtcta cgtccggccc gagatcgtgg cggaaatcgc cttcagtgat
      361 ttgcagtcga gcccgcgtta tgcgggaggt ctggcgctgc ggttggcacg cgtgaagcag
      421 tatcgtctgg acaagcgcgt cgaggacgct gacacgatgg agacggtacg caagctgttc
      481 gccgcgcagg gttagtccgc gtctaggtga agatcgcgat caggatgatg atcgggatgg
      541 ggacgccgat agcccacaag agtatggaac gcatgttgat ctccggatta cgaagcggcg
      601 agaaccgagg agtcgcgcag ccgcccgccg agcgtcgcgg cgaagctggc cacgaatgcg
      661 cccacgagca gcgataccac cagccacaag gcaagacccg ctgacgcctt tcgcgcgtcg
      721 tccgcgccct tcttgagctt gacgaaagtc tcgcccacgc gttgttccgc ggccgactgc
      781 gcaagcccgg tattcgccgc cacgacctgc cccaggtatt gacggtcctc atccgagagc
      841 tcgcccgact ggagcccatt cgcaaggatt cgcgtgactt cttcagttct atccggcgag
      901 acggccgtgt tgtcaggcgc gccgccccgg aacaacgagt cgatggaata gcccacggga
      961 ctggaatcgc cggccttcaa gccggtcgag acggccgacg ccaccgccga tgacagaagc
     1021 gttgccatga gcaacgtcgc caccgcccag gccaggaatc cgtgcgcggt atcgcggaag
     1081 tacacctcgt tggtgtgcac cgtgacccag cgcgtgcgca accggccggc gagataccca
     1141 cccaacaccg acgccagaag ctgaacgacg gtgacccaga cgatggtgga gacgccggct
     1201 gccttgccgg aaatgccctc gctctcccac ggcgaaacga ccgcgaggcc gaggcccgtg
     1261 cctaacacga acagcacgag cgaaagcgcc gcggcgccca cggctccggc gatcactgct
     1321 ccccaggaaa ccgcgctttc attgctggtt gtcatgccct gaaattgcgc gagttcccaa
     1381 agatgggatg caggcgctcc tcaggcggaa gtgtcagccg cgtcttacca gctcgaaacg
     1441 ccagaccaaa ccatcttcag ggtcgatcac ttcgcccatc ttctcgaagc cggacttgag
     1501 cagcacgcgt tgcgaagcga tgccgtccgg cagcgtgtgc gcgcgcacga cacaaacctg
     1561 gtccgacgcg agtgcgaatt ccgcgagcgc acgcgccgcg gccgttgcat ggcctttgcc
     1621 ctggtgctcc ggctcaatcg catacgcgat ttcgaccatg ccctcgaccg gcggtccctt
     1681 gaatgctccg agacccaccg tgacccccgc atcatcgacg acgtgaaagc cgtggaccca
     1741 cggatcctgc ggcgcggatt tctccaacag cgcgagccaa ttcggcgaga tctgcgcgcg
     1801 cacctcaggc gggagcacct gaagcgtggc gcggacctcg tcgcgggttc tgggaatcag
     1861 tcgcatgctc atcgcgagaa cacgacgccg tgcggcttgc cagtaccggt ccagccgtgc
     1921 ggccacttcc ggttcgccag atcgaaaccg ggtttgccgt tgtcatcctt gaacgcttcg
     1981 tcgaccgaga gcgcgccggt tttggcgtcc agcttcacca ggaacagccg attctccgag
     2041 cccgtcgtta ccagccgctt cgtcttcgca tcccatcccg tccagtgcgc atcgaaaccg
     2101 tcgctcaacg tgagacgcga gacttctttc ggcttctgcg ggttcgagat gtcgagcgcg
     2161 atcagaccat tgatcgccgg caccgactgg atcaggaaat tccccacgat cgtcggcacg
     2221 ccacaccagt ttccgggaaa cgtatgcacg agtttcgcga ccggtttcga tgtgagcccg
     2281 gtgatgcgtt cgatgccgca gcccagggtc tgcacgtaca cggagccatc ggccgcgact
     2341 cgcggctctt ccggagcgat atgcgcatag cggccctcgc ccggatcgaa atacgccgtc
     2401 tcgagtagtt tgaggtcgga gagacgccag acctggtagg tgacgccgct caacaggtcc
     2461 gggtcgtgca tcgatgaatt ggtggacacg atccggtcct gctccggcag aaccgccacg
     2521 ccgtaaggca tgagcagcgc atcgggaaaa gccggatccg ccgtgctcac ggatcgaacg
     2581 actttgccct tgtcatcgat ctccacgagg ccgcccgtgg cgccgagttc tccggcgtga
     2641 tggccatggt gcgcatgctg aaaggtcgcg atcacgttgc cattcggcaa acgcacgaac
     2701 gaatgcggat gggtgtagcc ggccatgtcg ccgaaggaag cagctaccct gggatgcagc
     2761 gggtcattca cgtcgaagat gaacgtgcgg cccacgtcgt gatcgttggc gaacaacatg
     2821 ccgctggccg gcatgacgta ttcggtgtga tggatgcgca ccgttttctg gtccgtgccg
     2881 acgctggcca ccagcttgcc gtagcccgtg gagttcgggt tcgcgtcgat gacggccagg
     2941 aagtcattgc cttccttctt ctcgtcgccc gtccaggcaa acaagtagtg gccctgtgcg
     3001 tgagcgggca gccaggccag gctggataac acgaggacaa acagcaaag
//
LOCUS       DATDEU010000576         4641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1426909, whole genome shotgun sequence.
ACCESSION   DATDEU010000576 DATDEU010000000
VERSION     DATDEU010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426909"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VIV63_13780"
     CDS             <1..520
                     /locus_tag="VIV63_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125717.1"
                     /translation="RLQWRKPPLPDPPPWREILELPPIIIDGQPTAIVVSRTEFLRAG
                     GANARRVAIVLHGVESLSGKENRAFSLELALAPDPKRIAAGDFRDVAPVDAFGGFELS
                     VLAEHADHHGASTALVLELPARALKALASAGDEFALLFFRRGTVDREGRPGAFDAKQP
                     LFRIGATRLAVQ"
     gene            complement(573..1499)
                     /locus_tag="VIV63_13785"
     CDS             complement(573..1499)
                     /locus_tag="VIV63_13785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6125718.1"
                     /translation="MNGFQGDFLRGALDVSPDGVVICETSGDNLVVYVNSAFCRLTGY
                     ETAELLGKNLRLLQGDDRDQQELDTLREALRHNEAVRVLVRNQRKDGTVFVNDMQIYP
                     LRNAEGKATHLIGYHREGGTRVRTGDTGIRGLPNWVREDRLTGLASRYYFEDVLKRDF
                     LLAQRDKTDIALLLFDIDQLGPYNEIFDRAGGDAVVKRVARAIAGSFRRGTDIVGRWE
                     GASVIVLMHGTTADHVIQHATTVAHRVREQQIHHPRSTQKYVTVSGGVAIHTARPEDS
                     ADKFLKAAEVAVARAKEQGRNRIIVAEESDFR"
     gene            complement(1496..2548)
                     /gene="nagZ"
                     /locus_tag="VIV63_13790"
     CDS             complement(1496..2548)
                     /gene="nagZ"
                     /locus_tag="VIV63_13790"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY6125719.1"
                     /translation="MTLGPLMVDVAGLELTPEDRDVLRHPLVGSVILFTRNYADPEQL
                     GRLVADIHAVRSPALVVAVDQEGGRVQRFRPGFSALPSPRRIGHEFDVDAKQGLAFAR
                     AMGWLMAAELRAYGVDLSFAPCVDLDLGVSEVIGDRAFHSKPEAVAQLAMAYMTGMKD
                     AGMAATAKHFPGHGAVVADSHKSLPVDRRPWNELGDELLPYRRLVANGIPGVMVAHIL
                     FPAVDPKPASLSRRWIQNALREELRFEGAVFTDDLSMGGAAEFGDIVARAKSALDAGC
                     DVLPVCNDRASVAKLLDELDFEVEPAAHLRLVRMRGRPAPERDALFAGDAWRQSQDLL
                     ARSAAAPKLNLTAGNA"
     gene            complement(2558..3895)
                     /gene="rlmD"
                     /locus_tag="VIV63_13795"
     CDS             complement(2558..3895)
                     /gene="rlmD"
                     /locus_tag="VIV63_13795"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF009639.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEY6125720.1"
                     /translation="MSKPAREGHAGIETGVVADLTHEAEGVVRASETGGKTVFVAGAL
                     PGEHIRFRRRSFHKSHDEAELLEIIEASPHRVTPRCAHFGVCGGCALQHLDPAAQIAA
                     KQKELAANLERIGNVTPAEWLPPLLGPHWNYRRRARLSSRFVAKKGRSLVGFREKQGK
                     YVADVQRCEVLAEPVASLVAALAELLTGMARRASIPQIEVALSDGERVLVVRVLDPLP
                     DADLEKLREFERAHGLRLLLQPGGLDTIAPLTPGPVDLHYRLDEFDVKLEFGPTDFVQ
                     VNAAINQAMVARAIELLEIGPTDRVLDLYCGLGNFTLPMARRAAGVVGVEGEASLIAR
                     ARANASLNGINNAQFHVADLSVAPDTTIPWMRGGFDKVLLDPPRVGAVKVLSAVAHLA
                     PRRVVYISCHTGSLARDLGVLTQELGFRLRAAGVLDMFPHTTHVESIAVLDRI"
     gene            complement(3892..>4641)
                     /locus_tag="VIV63_13800"
     CDS             complement(3892..>4641)
                     /locus_tag="VIV63_13800"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY6125721.1"
                     /translation="TVPDVEVGRRLYGLEGLSDDEVGKAMQAKARQDSGSDFLPHEQH
                     RIVAISCAFRSREGFRVWSLGDEGAPERELVERFFDGIDKFTPDLVSWNGGGFDLPVL
                     HYRALRFKVQAPRYWETGDEDNAFRYNNYLGRFHWRHMDVMDVLSGYQNRARASLSDT
                     AALLGFPGKLGFDGSQVWDAYRGGELTRIRRYCETDVINTWLIFLRFQHMRGRLTDPG
                     LADELTRTRTWLADAKQPHFDEFLAAWAPET"
BASE COUNT          773 a         1501 c         1585 g          782 t
ORIGIN      
        1 gcgactgcag tggcgcaagc caccgctgcc ggatccgccg ccatggcgcg agattctcga
       61 gttgccgccg atcatcatcg acggccagcc gacggccatc gtggtgtcac gaacggaatt
      121 cctgcgcgct ggcggcgcga acgcgcgccg cgtggccatt gtcctgcacg gcgtcgaaag
      181 cctgagcggc aaggagaatc gtgctttcag cctggagctg gcgctcgcgc ccgatcccaa
      241 gcgcattgcg gcgggcgact ttcgcgacgt cgcgccggtc gacgccttcg gcggcttcga
      301 actttcagtg ctcgcggagc acgccgatca tcacggcgcg tcgaccgccc tggtgctcga
      361 gctgcccgcg cgcgcgctca aggcgttggc gtcggcgggt gatgagttcg cgctgctgtt
      421 cttccggcgg ggcacagtcg accgcgaagg ccggccgggg gcgttcgacg ccaaacaacc
      481 gctgttccga atcggcgcga cgcgcctggc ggtgcagtag aagcgggttg aggtccggcc
      541 ccttttgcta gcaaatgggg agtgtcccca tttcagcgga agtcggattc ctcggcgacg
      601 atgatgcgat tgcggccctg ctctttggcg cgggccacgg cgacctcggc cgccttgagg
      661 aatttgtcgg ccgagtcttc gggacgcgcg gtgtggatcg cgacgccgcc gctcacggtc
      721 acgtatttct gcgtggaacg cgggtgatgg atctgctgtt cgcgcacgcg gtgcgccacg
      781 gtggtggcgt gctggatcac gtgatcggcc gtggtgccgt gcatcagcac gatcacgctg
      841 gcgccttccc agcggccgac gatatccgtg ccgcggcgga aggaacccgc gatcgcgcgg
      901 gccacgcgtt tgacgacggc gtcgccgccg gcgcggtcga agatctcgtt gtaagggcct
      961 aactggtcga tgtcgaacag cagcagtgcg atgtcggtct tgtcgcgctg ggcgagcagg
     1021 aagtcacgct tgagcacgtc ttcgaagtag tagcgcgacg ccagccccgt gaggcggtct
     1081 tcacgcaccc agttgggcag gccgcgaatg ccggtgtcgc cggtgcgcac gcgagtgccg
     1141 ccttcccggt gataaccgat caggtgagtc gccttgccct cggcgttgcg cagcgggtag
     1201 atctgcatgt cgttgacgaa caccgtgccg tccttgcgct ggtttcgcac cagcacgcgc
     1261 accgcttcgt tgtgccgcag cgcttcacgc agggtgtcga gctcctgctg gtcacggtcg
     1321 tcgccctgca gcagccgcag gttcttgcct aacaattcgg ccgtttcgta acccgtgagc
     1381 cggcagaacg cggaattgac gtacaccacg aggttgtcgc cggacgtttc gcagatgacc
     1441 acgccatccg gcgatacgtc gagcgcgccg cgcaggaagt ctccctggaa gccgttcatg
     1501 cgttgcccgc ggtcaggttg agtttgggcg cggccgccga gcgtgccagc aggtcctggc
     1561 tctggcgcca ggcgtcaccg gcaaacagcg catcgcgttc aggcgcggga cgcccgcgca
     1621 tgcggaccag ccgcagatgc gcggcgggct ccacttcgaa gtcgagttcg tcgagcagct
     1681 tcgcgaccga cgcgcggtcg ttgcacacgg gaagcacgtc gcagccggcg tcgagcgccg
     1741 atttcgcgcg ggcaacgatg tcgccgaatt cggcggcgcc acccatggac agatcgtcgg
     1801 tgaacaccgc gccctcgaag cgaagttctt cgcgcagtgc attctggatc cagcggcgcg
     1861 agaggctggc gggtttcgga tcgacggccg gaaacaggat gtgcgcgacc atgacgccgg
     1921 ggatgccgtt ggcgacgaga cggcgatagg gcaacagttc gtcgccgagt tcgttccacg
     1981 gccgcctatc taccggcagc gacttgtgcg aatcggccac gactgcgcca tgacccggaa
     2041 agtgtttggc ggtggccgcc atgccggcgt ccttcatacc ggtcatgtag gccatggcga
     2101 gttgcgcgac ggcttcgggc ttcgaatgga acgcgcgatc gccgatgact tcgctcacgc
     2161 ccaggtcgag atcgacgcag ggcgcgaacg acaggtcgac gccataggca cggagctcgg
     2221 cggccatgag ccagcccatg gcgcgggcga atgcgagacc ctgtttcgca tcgacgtcga
     2281 attcgtggcc gatgcggcgc ggcgacggca gcgccgagaa gccgggccgg aagcgctgga
     2341 cgcgaccgcc ctcctggtcc acggcgacga ccagcgccgg cgaccggacg gcatggatgt
     2401 cggcgaccag gcggcctagc tgttcgggat cggcgtagtt gcgtgtgaag aggatcacac
     2461 tacccacgag cggatggcgc agaacatcgc ggtcctcggg cgtcagctcg agaccggcga
     2521 catcgaccat cagtggtcca agggtcacaa aggctctcta aatcctgtcg agcacggcta
     2581 tcgattccac gtgcgtggtg tgcggaaaca tatcaagtac accggcggcg cgtaagcgga
     2641 agcccagttc ctgcgtcagg acacccaggt cgcgcgccaa ggaacccgtg tgacacgaga
     2701 tatacaccac cctgcgcggg gcgagatgtg ccacggcgct caaaaccttg acggcgccca
     2761 ctcgaggagg gtctaacagg accttgtcga accccccgcg catccaggga atggtcgtgt
     2821 cgggagccac agacaggtcc gcgacgtgga attgagcgtt gtttatgccg ttcaggctcg
     2881 cattggcgcg agcccgcgcg atgaggctgg cctcgccttc gacgcccacc acccccgcgg
     2941 cccgccgggc catgggcaac gtgaagttgc cgaggccgca atagaggtcc aggacccggt
     3001 cggtggggcc tatctccagt agttcgatgg cccgggcgac catggcctgg ttgatggcgg
     3061 cattcacctg gacgaagtcg gtgggcccga actcgagttt cacgtcgaac tcgtcgagcc
     3121 ggtaatgcag gtcgaccggc ccgggcgtca ggggcgcgat ggtgtccagg ccgccgggct
     3181 gcaacaggag gcgcagtccg tgggcccgct cgaactcgcg cagcttctcc agatcggcgt
     3241 cgggcagcgg gtcgagcacc cgaaccacca gcacgcgttc gccatcggac aacgcgactt
     3301 cgatctgcgg aatgctcgcc ctgcgcgcca taccggtgag cagttctgcc agcgctgcga
     3361 ccagtgacgc gaccggctcc gccagcactt cacatcgctg cacgtcggcg acgtacttgc
     3421 cctgcttctc gcggaagccg accaggctgc gacccttctt cgcgacgaaa cgcgaggaca
     3481 ggcgcgcacg acggcggtag ttccaatgcg ggcctaacaa gggcggcaac cactcggcgg
     3541 gcgtgacgtt gccgatgcgt tcgagattcg cggccagttc tttttgtttg gccgcgatct
     3601 gcgccgccgg atccaggtgc tgcaacgcgc agccaccgca gacgccgaag tgggcgcagc
     3661 gcggcgtcac gcgatgcggc gacgcttcga tgatttcgag aagttcggct tcgtcgtgac
     3721 tcttgtgaaa actgcggcgc cggaaccgga tgtgttcgcc cggcaacgcg ccggcgacga
     3781 acacggtctt gccgcccgtc tcgctggcgc gaaccacgcc ctcggcctca tgcgtgagat
     3841 cggcgaccac accggtctcg atgccggcat ggccttcgcg cgccggcttg ctcacgtctc
     3901 gggcgcccag gcggcgagga attcgtcgaa gtgtggttgt ttggcgtcgg cgagccaggt
     3961 gcgcgtgcgc gtgagttcgt cggcgaggcc cgggtcggtg agccgcccgc gcatgtgctg
     4021 gaagcgcagg aagatgagcc aggtgttgat gacgtcggtt tcgcagtagc ggcggatgcg
     4081 cgtgagctca ccaccgcgat aggcatccca cacctgcgaa ccatcgaagc cgagtttgcc
     4141 cggaaagccg agcagcgccg cggtgtcgga caatgatgcg cgggcgcggt tctggtagcc
     4201 cgacagcacg tccatgacgt ccatgtgtcg ccagtgaaag cgcccgagat agttgttgta
     4261 acgaaaggcg ttgtcttcgt cgccggtttc ccagtaacgc ggcgcctgca ccttgaaacg
     4321 cagcgcgcgg taatgcagca cgggcagatc gaagccgccg ccgttccagc tcacgagatc
     4381 gggcgtgaac ttgtcgatgc cgtcgaagaa ccgttcgacc agctcgcgtt ccggcgcgcc
     4441 ctcgtccccc aggctccaga cgcggaaacc ttcgcgcgag cgaaacgcac aggagatggc
     4501 gacgatgcgg tgctgttcgt gcggcaggaa gtcgctgccc gaatcctggc gcgccttcgc
     4561 ctgcatggcc ttgccgactt catcgtcgga caaaccttcg agcccgtaga gacgccgccc
     4621 gacttccaca tccggaaccg t
//
LOCUS       DATDEU010000577         4067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1572362, whole genome shotgun sequence.
ACCESSION   DATDEU010000577 DATDEU010000000
VERSION     DATDEU010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572362"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VIV63_13805"
     CDS             <1..284
                     /locus_tag="VIV63_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY6125722.1"
                     /translation="NVVAKSPSRFATAVRAPILLIHGVDDTVVPIAQSQRMARALREA
                     NKPHEIIELAGEDHWMMTNSSSRIRTLTELERFLGQYMTGEAPAKQAAN"
     gene            332..683
                     /gene="ssrA"
                     /locus_tag="VIV63_13810"
     tmRNA           332..683
                     /gene="ssrA"
                     /locus_tag="VIV63_13810"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            918..2117
                     /locus_tag="VIV63_13815"
     CDS             918..2117
                     /locus_tag="VIV63_13815"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY6125723.1"
                     /translation="MNRKATRFLALAGALAPSLASATLLINDGGTHSILAATAQSVLV
                     DNGSELRIAGGGSITGDGTNTSSSLARGALSAGPLSNSTIRLSDSAQVFAGSDQYAIA
                     RSLGGRLYLSGDSQVHGGIFGFATTSPPTSPTLRTYLSDNAVVNGNVDTDGLVSLSGN
                     SLITGHLFENNGGIALEMNGGTIQGRVSTSSLVGHSALINDGAILGGYGGNASNIDFS
                     IHGGLIDGGWSVYSQDMLVELRGGQINGGMLFGSIADPNATGDRVSIFSGAIDATAGG
                     WLLDFASGFDFGGATSFDCSANTTRFDIWGGQLGGSSAGNGIRLDYCATLDVYGTGLS
                     YSGGWLTGLLADGSLLNVAVSEVDRWGGAIRLHDVSVPEPGTLGLLGLALGAMAMTRR
                     RRAQANS"
     gene            complement(2123..2347)
                     /locus_tag="VIV63_13820"
     CDS             complement(2123..2347)
                     /locus_tag="VIV63_13820"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY6125724.1"
                     /translation="MGNEAEELAEIDALFAHADKDHDGQINFTEFRSLIEELDGQMTD
                     AQLRIGFTETDTNHNGRINIDEFREWWLSD"
     gene            complement(2337..3260)
                     /gene="aguB"
                     /locus_tag="VIV63_13825"
     CDS             complement(2337..3260)
                     /gene="aguB"
                     /locus_tag="VIV63_13825"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEY6125725.1"
                     /translation="MTVPAHLNRPNREVVVAATQFACGWDVAANIANAEKLVRQAAKQ
                     GAQIILIQELFETPYFCIEQDSRHLRIATTVADNLAIKHFAKIARELDVVLPISFFEK
                     ANNSFFNSIAILDADGSNLGVYRKAHIPNGPGYQEKNYFSPGDSGFKVWNTKYARIGV
                     GICWDQWFPECARAMALMGAELLFYPTAIGSEPPPAMPVSSRDHWQRCQQGHAAANLM
                     PVIVSNRIGTERALQDPEGLYIRFYGSSFIADSTGAKIAEAGEEGEAVLTAKFDLAAN
                     EELRNNWFVFRDRRPELYGALTSYDGSQHGK"
     gene            complement(3257..>4067)
                     /locus_tag="VIV63_13830"
     CDS             complement(3257..>4067)
                     /locus_tag="VIV63_13830"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6125726.1"
                     /translation="KQLFIGSEGTLGVVTRAELRLVSRTRSQETMLAALPTLDAVVEL
                     LGRLDSGLGGQLAAFEALWGNYYDYNTAPPASNNAPLARGAPFYAIAETLGGDPEHDR
                     ARVESVLTQALEDGLLTDVTIASSETERRAIWAIREDVWQVKNIAPLLTFDVSLPIEN
                     MKAYTEEVCADVHAFAGPNRAFVFGHMADGNLHLVIAAGDDAVTRAKVENMVYRPLAA
                     IGGSVSAEHGIGLEKRDYLPLSRTAAEISTMRLLKNALDPKGILNPGKVFA"
BASE COUNT          736 a         1302 c         1236 g          793 t
ORIGIN      
        1 cgaacgtggt tgcgaagtca ccctcgcgat tcgcgaccgc ggttcgagcg ccgatcctgc
       61 tcattcacgg cgtcgacgac accgtcgtgc cgatcgcgca atcgcaacgc atggcccgcg
      121 cgctccggga ggccaacaag ccccacgaaa tcatcgaact ggcgggagag gaccactgga
      181 tgatgaccaa cagttcctcg cgcatccgca ctctcactga actcgagcga ttcctcggcc
      241 aatacatgac aggcgaggct ccagccaagc aggccgccaa ctgagttgca gacgcgcacc
      301 ctgcccccat gtatgctcgc atcaccttta cgggggcgac cggtttcgac gtgggtagtg
      361 aaccgtaaga ggcgtaccga ggcgcatggc cctcgttaat aactttgcac tttacagttg
      421 ccaacgacga caactacgca ctagccgcct aatcgcggtt gccctcagac cagttggtgc
      481 ctatgccact gaacctgggg tagcgctcat aggatcgcgt ttccgtgtgc tactggcgga
      541 atcgttaaga gtcacagtcg ctggccgagc gttttccctg ctcttcgggt agcgtgaggc
      601 gagaaaataa agaagcagct acgtacgtag aatcttacgt ctaacgcttg cggacgcgag
      661 ttcaattctc gccgcctcca ccaaatctcc cggcgcgccg gcgccgggag tgcatgcaaa
      721 aatttcaatt gcatcgcgcg cgcgacgaat cccgtatcgc gcgaaataaa actttcgaac
      781 tgaatcacag tttcggtccg ctcagacttt tccctacgac ttgaaacccg gattgcactc
      841 gtacactcgc gtgcggcgat gcagtttctc gggtagttag accgccgcgg cttaacataa
      901 gatcataggg aagtgtcatg aacaggaagg cgacgcgttt tctcgcactg gccggcgccc
      961 tggcgccctc actcgcatcg gcgacgctgc tcatcaacga cggcggcacc cattcaatcc
     1021 ttgcggcaac tgcacaaagc gtgctggtcg acaacggctc ggagcttcgc atcgccggcg
     1081 gcggctccat taccggagat ggcaccaata ccagttcgtc gctcgctcgc ggtgcgttgt
     1141 ccgccgggcc gctgagcaac agcaccattc gcctgtccga cagcgctcag gtcttcgccg
     1201 gcagcgatca gtacgcgatc gcgcgatcgc tgggcggccg gctctatttg tccggcgaca
     1261 gccaggtgca cggcggcatc ttcggattcg ccaccacctc cccgcccact tctccgactc
     1321 tgcgcacgta cctgtcggac aacgcggtgg tcaacggcaa cgtggacacg gacgggctgg
     1381 tatcgctttc cggaaactcg ctgatcaccg gacacctgtt cgagaacaac ggtgggattg
     1441 cgctcgagat gaacggcggc accatccagg gacgcgtcag cacgagctcc ctcgtcggtc
     1501 attccgcact gatcaacgac ggcgcgattc tcggcgggta cggcggcaat gccagcaaca
     1561 tcgatttttc gatacatggc ggcctcatcg acggcggctg gagcgtgtat tcgcaggata
     1621 tgctggtgga actgcgcggc ggccagatca acggcggaat gctcttcggc agcattgccg
     1681 atcccaacgc gaccggcgac cgggtttcga tcttcagcgg cgccatcgac gccaccgcgg
     1741 gcggctggct actcgacttc gcgtcgggct tcgatttcgg cggcgccacg tccttcgact
     1801 gcagcgccaa caccacgcgc ttcgatatct ggggtggtca actcggcgga agctccgctg
     1861 gcaatggcat ccgcctcgac tattgcgcga cgctcgacgt gtacggaacc ggcctgagtt
     1921 attcgggtgg gtggctcacc ggtcttctcg cggatggcag cctgctcaac gtcgcggttt
     1981 ccgaagtgga tcgctggggc ggcgcgattc gcctgcacga cgtcagtgtt ccggaacccg
     2041 ggacgctggg cctgctcggc ctggcgctgg gcgcgatggc gatgacacgc cgacgccgcg
     2101 cgcaagcaaa ctcataagcc catcaatcgc tgagccacca ctcgcgaaac tcatcgatgt
     2161 tgatgcgccc gttgtggttc gtgtcggttt cggtaaagcc gatacgcaac tgcgcgtcgg
     2221 tcatctgacc atcgagttcc tcgatcagtg accggaactc ggtgaaattg atctggccgt
     2281 cgtgatcctt gtccgcgtgc gcaaacaggg catcgatttc cgccagttct tctgcctcat
     2341 ttcccatgtt gacttccgtc gtagctagtt agcgcgccgt acaactccgg ccgccggtcg
     2401 cgaaacacga accagttgtt gcgcagttct tcgttggctg cgaggtcgaa cttcgcggtg
     2461 agtacggctt caccttcctc gcccgcctcg gcgattttcg cgcccgtgga gtcggcgatg
     2521 aagctcgatc cgtagaagcg gatgtacagg ccttcgggat cctgcagtgc gcgctcggtg
     2581 ccgatccgat tcgagacgat gaccggcatc aggttcgccg ccgcgtgacc ctgctggcaa
     2641 cgctgccaat ggtcgcgcga actgaccggc atcgcgggag ggggctcact tccgatcgcc
     2701 gtcgggtaga acagcaactc cgcgcccatg agcgccatgg cccgcgcgca ctccggaaac
     2761 cactggtccc aacagatgcc cacgccgatg cgcgcgtact tcgtgttcca gactttgaag
     2821 ccggaatctc ccgggctgaa atagttcttc tcctggtatc caggcccgtt gggaatatgc
     2881 gccttgcgat acacgccgag attgctcccg tccgcgtcga ggatggcgat ggaattgaag
     2941 aacgaattat tggccttctc gaagaaactg atcggcagca ccacatcgag ctcgcgcgcg
     3001 atcttcgcga agtgtttgat ggccaggttg tccgccacgg tggtggcgat gcgcagatgg
     3061 cgcgaatcct gctcgatgca gaaatagggg gtttcgaaca gctcctggat caggatgatc
     3121 tgcgcgccct gcttcgccgc ctggcgcacg agtttctcgg cgttggcgat gtttgccgcc
     3181 acgtcccagc cgcaggcgaa ctgggtggcc gccacgacca cttcgcgatt cggccgattc
     3241 aaatgtgctg gaacggtcat gcgaacacct tgccgggatt gaggatgcct ttgggatcga
     3301 gcgcgttctt gagcagccgc atggtggaaa tctccgcggc cgtgcgcgac agcggtagat
     3361 agtcacgttt ttcgagcccg atgccatgct cggcggacac agacccgccg atggcggcga
     3421 gcggccggta gaccatgttc tcgaccttgg cgcgggtcac ggcatcgtcg cccgcggcga
     3481 tcaccaggtg cagattgccg tccgccatgt ggccgaacac gaacgcccgg ttcggtcccg
     3541 cgaacgcgtg cacgtcggca cacacctctt ccgtgtacgc cttcatgttt tcaatgggca
     3601 ggctcacgtc gaacgtgagc agcggcgcga tgttcttgac ctgccagaca tcttcgcgaa
     3661 tcgcccagat ggcgcgccgc tcggtctcgg aactggcaat ggtgacgtcg gtgagcagcc
     3721 cgtcctcgag cgcctgcgtg agcacactct cgacccgcgc gcggtcgtgc tcgggatctc
     3781 cgcccagtgt ttccgcgatc gcgtagaacg gcgcaccgcg cgcgagcggc gcgttgttcg
     3841 acgcgggtgg cgccgtgttg tagtcgtagt agttgcccca cagggcctcg aacgcagcga
     3901 gctgcccgcc aagtccggaa tcgagccggc ctagcagttc gaccaccgcg tcgagcgtgg
     3961 gcagcgccgc gagcatggtt tcctggctgc gcgtgcggga taccaggcgc aactcggcgc
     4021 gcgtcaccac gcccagagtg ccctcgctgc cgatgaagag ctgcttg
//
LOCUS       DATDEU010000578         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1718502, whole genome shotgun sequence.
ACCESSION   DATDEU010000578 DATDEU010000000
VERSION     DATDEU010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718502"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1077
                     /locus_tag="VIV63_13835"
     CDS             130..1077
                     /locus_tag="VIV63_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125727.1"
                     /translation="MIAGERLARYGFGDGHPFGPDRHAAFLAEFSARGLDKRVRTMET
                     RAATREELQTFHTPEYLDLVADRSRTGDGYLDGGDTPAWRGVYEAAADVVGATLLAAE
                     QIMSGKARRAFIPIAGLHHAARGGAAGFCVFNDCGVAVEMLRKNHGLKRIAYVDIDAH
                     HGDGMYYGFESDPDLIFADIHEDGRHLYPGTGSETETGRGAAAGTKLNIPVAPGSDDT
                     VFDAEWPRVLAHLRKFKPEFILLQAGADSVEGDPITHMRFTAATHGRAARDLAALAEE
                     LGHGRVLGTGGGGYNRINLAQAWTSVVQGFVDTPVPEHH"
     gene            1179..2435
                     /gene="glyA"
                     /locus_tag="VIV63_13840"
     CDS             1179..2435
                     /gene="glyA"
                     /locus_tag="VIV63_13840"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY6125728.1"
                     /translation="MFRTAMTIGGFDPELETALKNERQRQEDHIELIASENYVSPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDVAEQLAIDRAKKLFGADYANVQPHSGSQAN
                     AAAYLALINPGDTILGMSLDHGGHLTHGAKVNFSGKLFRAVQYGIRPDNGEIDYDALA
                     AQALAEKPRMIIAGFSAYSRYLDFRKFREIADSVGAYLFVDMAHVAGLVAAGLYPDPI
                     PYADVVTTTTHKTLRGPRGGLILARANDAITKKLNSLVFPGTQGGPLMHVIAAKAVAF
                     LEALQPEFKEYQKQVVANARAAATRLQKRNYKIVSGGTDNHLFLLDLSDKAINGKEAD
                     AALGKAHITVNKNAVPNDPRPPMVTSGIRIGSPAGTTRGFKEPEFEQIADWIADVLDA
                     NGSDAAIDKARAEASELCRRYPVYGP"
BASE COUNT          470 a          815 c          757 g          424 t
ORIGIN      
        1 gcgggaaagg cgcggaattc tacatccccc gcccgtgttc ctgtgctacg tttgcccgcg
       61 ttttcaagtt cgatggggag agcgccactg tccactgcag cgtcatccgg cctgctgcct
      121 ccggttctat tgatcgctgg cgaacgcctg gcgcgatatg gcttcggcga cggccatcct
      181 ttcggtccgg atcgccatgc cgcgttcctc gcggagttca gtgcgcgcgg tctcgacaag
      241 cgggtgcgta ccatggaaac gcgggcggcc acacgcgagg aattgcagac ttttcatacg
      301 ccggaatatc tcgacctggt cgcggaccgc tcgcggaccg gcgatggata tctcgatggc
      361 ggcgacactc cggcctggcg cggtgtctac gaagccgcgg ccgacgtggt gggtgccacg
      421 ctgctcgccg ccgaacaaat tatgtcgggc aaggcgcgtc gcgccttcat tcccatcgcg
      481 ggcctccacc acgcggcgcg cggcggcgcg gcgggcttct gcgttttcaa cgactgcgga
      541 gtggcggtgg aaatgctgcg caagaatcac ggtttgaagc gcattgctta cgtggatatc
      601 gacgcgcacc acggcgacgg aatgtattac ggcttcgaaa gcgatcccga cctcatattc
      661 gccgacatcc acgaagacgg ccgtcacctc tatccaggca cgggtagcga gaccgaaacc
      721 ggtcgcggcg ccgccgcggg caccaagctc aatattcccg tcgcgcccgg atccgacgac
      781 acggtcttcg acgccgagtg gccccgcgtg ctggcccatc tgcgcaagtt caagccggaa
      841 ttcatcctgc tccaggcggg ggccgacagc gtcgaaggcg accccatcac ccacatgcgt
      901 ttcaccgccg cgacacacgg ccgcgcggcg cgcgatctgg cggcgctggc cgaagaactg
      961 gggcacgggc gcgtgctcgg aacgggtggt ggtggctaca accgcatcaa cctggcgcag
     1021 gcctggacgt cggtcgttca gggattcgtc gatacgcccg ttccggaaca ccactaaaca
     1081 caactaatag tgtttaccca gcctcttttc tccagagggg ccggcacatc cagtacaatt
     1141 cgcggccccc ggaagttcga ccccgtgagg ccctttccat gttccgtacc gcgatgacca
     1201 tcggtggttt cgaccccgaa ttagagaccg ctctcaagaa cgaacgccag cgccaggaag
     1261 atcacatcga gctgatcgcc tcggaaaact acgtcagccc gcgcgttctc gaagcgcaag
     1321 ggtcggtgct caccaacaag tacgccgaag gttatccggg caagcgttat tacggcggtt
     1381 gcgaattcgt cgacgtcgcc gagcagctcg ccatcgaccg cgccaagaaa ctcttcggcg
     1441 ccgactacgc caacgttcaa ccgcactcgg gtagtcaggc gaatgccgcg gcatatctcg
     1501 cgttgatcaa tccaggcgac acgatcctcg gcatgagcct cgatcatggc ggccatctca
     1561 cgcacggcgc caaggtcaat ttctccggca agcttttccg cgcggtgcag tacggcatcc
     1621 gccccgacaa tggcgagatc gactacgacg cactcgctgc gcaggcgctg gctgaaaaac
     1681 ccagaatgat catcgcgggt ttctccgcct actcgcgtta tctcgacttc aggaaattcc
     1741 gcgagatcgc cgattcggtc ggcgcgtacc tgttcgtcga catggcgcac gtcgcgggcc
     1801 tcgtggccgc gggcctgtat ccggatccga ttccgtacgc cgatgtggta actaccacca
     1861 cgcacaagac gctgcgcggt ccgcgcggtg gtttgatcct ggcgcgcgcc aacgacgcga
     1921 tcacgaagaa gctgaactcg ctggtgttcc ccggcacgca gggcggcccg ctgatgcacg
     1981 tcatcgcggc gaaagccgtg gcgttcctcg aagcattgca gcctgaattc aaggaatacc
     2041 agaagcaggt ggtggcgaat gcacgcgcgg ccgccacgcg gctgcagaag cgcaactaca
     2101 agatcgtctc cggcggcacg gacaatcacc tgttcctgct cgatctctcc gacaaggcga
     2161 tcaacggcaa ggaagcggat gcggcgctcg gcaaggcgca catcaccgtc aacaagaacg
     2221 ccgtgccgaa cgatccgcgt ccgccgatgg tcacgagcgg catccgcatc ggctcgccgg
     2281 ccggcacgac gcgcggtttc aaggagcccg agttcgagca gatcgcggac tggatcgcgg
     2341 acgtgctcga tgcgaacggc agcgacgcgg ccatcgacaa ggcgcgcgcc gaggccagcg
     2401 aactctgccg tcgttatccg gtgtacggac cgtaagccat aacgagggcg tgccatgcac
     2461 tgtccc
//
LOCUS       DATDEU010000579         8506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1572903, whole genome shotgun sequence.
ACCESSION   DATDEU010000579 DATDEU010000000
VERSION     DATDEU010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572903"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2505)
                     /gene="polA"
                     /locus_tag="VIV63_13845"
     CDS             complement(<1..2505)
                     /gene="polA"
                     /locus_tag="VIV63_13845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY6125729.1"
                     /translation="MNSRPDLVLVDGSSYLYRAFHALPPFSNSRGEPTGAVFGVLNML
                     AKFLKDYDPQSIAVVFDAPGKTFRDELFAEYKAHRPPMPDDLRSQIEPLFEAVRGMGL
                     PILRETGVEADDVIGTLAITAAKENLSVLISTGDKDMAQLVSPQITLINTMSGTVLDR
                     AGVKTKFDVFPEQIIDYLALVGDSSDNIPGIDKVGPKTAAKWLGKYSTLDALVTDAAN
                     VEGKVGENLRAGLATLELARKLATIRTDVPLPVTLSELKRSPPNVDALRALYTRLELR
                     SLLKQLDGGAAPSASAETAAPAGQPASAAPSTAGVSDAAQAVAAAPRQYETITTEAQL
                     DAWVKKLEAAELFAFDTETTSLEYMKAEIVGVSFAVAAGIAAYLPLRHDYAGAPDQLD
                     RDASLAKLKPLLESPRHAKVGHHLKYDAHVLANHGIRLDGMRFDSMLESYVWNSVATR
                     HDMDSAAERYLGIRTIHYEDVAGKGAKQISFSQVSVDVAAEYSSEDSDVTLRLHQVLW
                     PQISAVPRLRQLYEECEQPLVPVLIRMEEHGVLLDRQMLRVQSTELGKRLLEVLGEAH
                     REAGAPFNLDSPKQLCNILFEKMQLPVIRKTPTGQPSTAEDVLEELAESYPLPKLILE
                     HRSLSKLKSTYTDKLPEQINARTGRVHTSYHQAVAATGRLSSQDPNLQNIPIRTPEGR
                     RIRQAFVAPEGHVLLAADYSQIELRIMAHLSQDEGLLKAFASDKDIHQATAAEVFGAT
                     LETVTADQRRSAKAINFGLIYGMSAFGLAKQLGIPRNAAQEYIDLYFARYPGVKRYME
                     ETRAQAKAQGYVETVFGRRLYLPDINARNKQFQQAAE"
     gene            complement(2522..2782)
                     /locus_tag="VIV63_13850"
     CDS             complement(2522..2782)
                     /locus_tag="VIV63_13850"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEY6125730.1"
                     /translation="MRNVIPVLILGILAGCGQKGPLYLPEERGEVVTRPAETPPESET
                     TAAPNSPQTPDSQPAAPAPAPEVTAPEEESKKDKGASPPPPS"
     gene            complement(2775..3242)
                     /locus_tag="VIV63_13855"
     CDS             complement(2775..3242)
                     /locus_tag="VIV63_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125731.1"
                     /translation="MATQMNEHPLTGYTHVMYALHALAGFIGVTSGASIVGAFVFGLP
                     SILAIIMNYARRDAVRGSFLESHFRWQATTFWTAIVLGVALFCISMVLTMIGVISLGS
                     AVITGAAGAAVAGASFAGALASWILGAILAGVWILYRVVRGWMALGGGKAMHA"
     gene            complement(3227..3658)
                     /locus_tag="VIV63_13860"
     CDS             complement(3227..3658)
                     /locus_tag="VIV63_13860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEY6125732.1"
                     /translation="MAIWFGNPTIDDARASQVGLLAPHLGIEFTEWGDDFLRGTMPVE
                     PRTRQPMGYLHGGASLVLAETLGSVAANFVVDFAKFRCLGQEINANHLRPVSDGLVIG
                     TARPFHLGSRSQVWSIEIRDPRERLVCISRLTVAVVEWRPK"
     gene            complement(3674..4858)
                     /gene="argH"
                     /locus_tag="VIV63_13865"
     CDS             complement(3674..4858)
                     /gene="argH"
                     /locus_tag="VIV63_13865"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY6125733.1"
                     /translation="MSRLWDKGVDLDQRVLAYTAGDDYALDERLVEYDVRASIAHAEM
                     LNAQKLLSDADLAAIRDGLTAIGAEHAAGQWKIELADEDGQTALEKRLTARIGAAGGR
                     VHLGRSRNDQVLTAVRLYLLDAGRVLAAAAEGCAAALDGLAARDGAIVLPGYTHMQQA
                     MPSSVALWAQGFAAELRDDAQGLASISRRASRSPLGSAAGYGTPGLPLDRELTRARLG
                     FAEVHQPVTAVQLARGKAESQLLFECTLLMQDLGRLAADLLLFYTQEFAFVSLPASFT
                     TGSSIMPQKRNPDVFELVRGRSAVAQACLFEVLGIFAKLPSGYQRDLQLIKPPLFRGI
                     DLALSTAAIMSIAMDGVRFRSDQITLDPAIHAAEEANALVAREGIPFREAYRRIGEKY
                     RK"
     gene            complement(4867..5802)
                     /gene="argB"
                     /locus_tag="VIV63_13870"
     CDS             complement(4867..5802)
                     /gene="argB"
                     /locus_tag="VIV63_13870"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY6125734.1"
                     /translation="MIMHRPDPSAAIRSLRSAAPYIRMYKGKTFVVKAGGGVFADPAA
                     VRGLIEQIAILHYFGVRVVFVHGGGPQLTEITEALGVPTRMVQGRRVTDQKAIDATSM
                     VLNGLINTRLLAFCREMDIDAVGISGVDAGLIKAHKRPPVRLADSGETVDYGFVGDID
                     SIDTTVIRKLLDNGLMPVVSPLSADENGTLLNINADTVAAAIGAALSAEKLMLCTGAP
                     GILERVDDPTSIISYTDLNGLKRLREEKKIVDGMLPKAKAIEDAIRGGVRRVHVMSYK
                     SPEGILAEVFTNEGAGTLIVADVNALSPAEQQVDK"
     gene            complement(5837..6844)
                     /locus_tag="VIV63_13875"
     CDS             complement(5837..6844)
                     /locus_tag="VIV63_13875"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HEY6125735.1"
                     /translation="MKNFLDLADMSREQVVELLALAERLAQKPEPHALAGKILGLLFF
                     NPSLRTLASMQAAMARLGGNSFVISAGQGTWQLETRRGAVMNGAPAEHVREGVPVLAH
                     YCDALGIRAFAGGADLEADLNETAFKEMVGLVSKPVVNMESAANHPCQALADWKTLDD
                     LTVPRKARFVLSWVNHPRALPLAVPAATVHMAAMRGMEVVVLRPEGFALPAPVMQKAA
                     QAAAAAGGSVRETSDRDDALSGAHVVYAKEWGSTSHYGNAEADARQRAQLADWCVRDT
                     WFSRAAVDCKLMHCLPVRRNVAVADEVLDGRRSVVQREAYNRMVVQTAVLHRLLSTPQ
                     H"
     gene            complement(6841..8004)
                     /locus_tag="VIV63_13880"
     CDS             complement(6841..8004)
                     /locus_tag="VIV63_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6125736.1"
                     /translation="MNAELKNTSPADGLAPVYAQYPIEVVGADGVWLHTRDGRKVLDL
                     YGGHAVASLGYGHKGWTKALSDQAAQMNFQTNAVPMEIRARAAHKLLSFSKLPFDSVF
                     WINSGAEANENAFKLAFKMRPGRTHVAAVEQSFHGRTAACASTTWQAPQKWFFLPRAP
                     FDVSWIKRRDVADIANHVTENTAAVIVEPVQGVGGAFDMGQEFLAALRSRCNETGALL
                     IFDEVQCGVGRTGQPFAANYYGVMPDMITTAKALGNGFPVSALLLTPPITASLKLENL
                     GTTFGGGPMACAVAEATIDAIEKEDLLANVRRVSAYLTKTCAVGPVVGFQGAGVLAGL
                     KMNKPAKDIQKALLAKDILAGTAGDPSILRLLPAFILKEEHVDRLRAALIDLA"
     gene            complement(8028..>8506)
                     /locus_tag="VIV63_13885"
     CDS             complement(8028..>8506)
                     /locus_tag="VIV63_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125737.1"
                     /translation="RKPTEGTHHPTRHSDLYSYGALGHRHTPEITALAKAASGVEAEF
                     AFVPHSGPFARGIHATVQAKLKSPRDAASAVAMLREYYAGCPFVRVLDNAPRVKDIVA
                     SNYAHLTAVANGRTIAVMSVVDNLNKGAAGGAVQWMNRLFNLPETAGLTAPAPGWT"
BASE COUNT         1387 a         2691 c         2911 g         1517 t
ORIGIN      
        1 ttctgctgcc tgctggaact gcttgttgcg cgcgttgatg tccggaagat agaggcgccg
       61 cccgaacacg gtctcgacat acccttgcgc cttggcctgc gcgcgcgtct cttccatata
      121 acgcttcacg cccggataac gcgcgaaata caggtcgatg tattcctgcg cggcattgcg
      181 cggaatgcct aactgcttgg cgagcccgaa cgcgctcatg ccgtagatga gtccgaagtt
      241 gatcgccttg gcggagcgtc gctggtccgc ggtgaccgtt tcgagcgttg cgccgaaaac
      301 ttccgcggcg gtagcctgat ggatgtcctt gtcactcgcg aatgccttga gcaggccttc
      361 gtcctgcgag aggtgcgcca tgatgcgcag ctcgatctgc gagtagtccg ccgcgagcag
      421 gacatgcccc tcgggcgcca cgaatgcctg gcggatgcgg cgcccctcgg gggtgcggat
      481 cgggatgttc tgcaggttcg gatcctgcga cgacaggcgg ccggtggcgg ccacggcctg
      541 gtgatagctg gtgtgcacgc ggccggtacg cgcgttgatc tgctcgggta gtttgtccgt
      601 gtacgtggac ttgagtttcg agaggctgcg gtgctcgagg atgagtttag gcagcgggta
      661 actctccgcg agctcctcga gtacgtcctc ggcggtcgaa ggctggcccg tcggtgtctt
      721 gcggatcacc gggagctgca tcttctcgaa caggatattg cagagctgct tgggtgaatc
      781 gagattgaag ggcgcgccgg cttcgcggtg agcctcgccc agcacttcga ggaggcgctt
      841 gccgagttcc gtgctctgga ctcgcagcat ctgccggtcg agcaatacac catgctcttc
      901 catgcggatg agcaccggca cgagcggctg ttcgcattcc tcataaagtt ggcgcagtcg
      961 cggaaccgcg gagatctgcg gccacagcac ctggtgcagt cgcagcgtta cgtcggagtc
     1021 ttcggacgaa tactcggcgg cgacgtccac cgagacctgg ctgaacgaaa tctgcttcgc
     1081 gcctttgccg gccacgtctt cataatgaat ggtgcgaatg ccgaggtagc gttccgcggc
     1141 cgagtccatg tcgtggcgag tcgccacgct gttccagaca taggactcga gcatcgagtc
     1201 gaaacgcatg ccgtccagcc ggatgccgtg gttggccagc acgtgcgcat cgtacttcag
     1261 gtgatgaccc accttcgcgt gccgcggaga ttcgagcagc ggtttgagct tcgcgaggct
     1321 cgcgtcgcga tcgagctgat cgggtgcgcc cgcgtagtcg tggcgcagtg gcagatacgc
     1381 ggcgatgccg gcggccacgg caaacgagac gcccacgatc tccgccttca tgtattcgag
     1441 gctcgtggtc tcggtgtcga acgcgaagag ctcggccgcc tcgagcttct tcacccacgc
     1501 gtcgagctgg gcttcggtcg tgatggtttc gtattgacga ggcgcggccg cgacggcctg
     1561 ggcggcatca ctgacgccgg cggtcgatgg cgccgcactc gccggctgtc ccgccggggc
     1621 tgcggtctcg gccgacgccg agggcgcagc gccgccgtca agttgcttga gcagcgagcg
     1681 aagttcgagg cgcgtgtaca gcgcgcgcaa cgcgtcgaca ttcggcgggc tgcgtttgag
     1741 ctccgataac gtgacgggca gcgggacatc cgtgcggatg gtcgcgagct tgcgagccag
     1801 ctcgagcgtc gcgaggcccg cgcgcaagtt ctcgccgacc ttgccttcga cattggcggc
     1861 gtcggtcacc agggcatcga gcgtcgaata cttccctaac cacttcgccg ccgtcttcgg
     1921 gccgaccttg tcgatgcccg gaatgttgtc ggagctgtcg cccacgagcg cgaggtagtc
     1981 gatgatctgt tcgggaaaca cgtcgaactt ggttttcacg ccggcgcggt ccagcaccgt
     2041 gccgctcatg gtgttgatca gcgtgatctg cggcgagacg agctgcgcca tgtccttgtc
     2101 gcccgtcgaa atgagaacgc tgaggttctc tttcgcagcc gtgatggcca gcgtgccgat
     2161 cacgtcgtcg gcttccacgc ccgtctcgcg caggatgggc agacccatgc cacgcacggc
     2221 ttcgaacagc ggctcgatct gcgaccgcag gtcgtccggc atgggtggac gatgcgcctt
     2281 gtactcggcg aacaattcgt cgcgaaacgt cttgccgggc gcatcgaaca cgacggcgat
     2341 gctctggggg tcgtagtcct tgaggaactt cgcgagcatg ttcagcacgc cgaataccgc
     2401 cccggtgggt tcgccgcgcg aattgctgaa gggcggcaac gcgtggaaag cgcgatacag
     2461 gtacgacgag ccgtcgacga ggacgagatc cgggcgggag ttcatgcgag cgactgtccg
     2521 ttcaactggg cgggggcggc gaagcgcctt tgtctttctt cgattcttct tcgggcgccg
     2581 tgacttcggg agccggcgcc ggagctgccg gttgcgagtc cggcgtctgc ggagaattgg
     2641 gtgccgcggt cgtttccgac tcgggcggcg tctcggcggg ccgcgtgacc acctcgcccc
     2701 tttcctcggg tagatagagc ggacctttct gcccgcatcc ggcgaggatg cccaggatca
     2761 gcactgggat aacgttacgc atgcatggcc tttccgccgc ccagcgccat ccatccgcgc
     2821 accacgcggt acaggatcca gacgccggcg agaatggcgc cgaggatcca ggacgccagc
     2881 gcgccggcaa aactcgcacc ggcgacggca gcacccgcgg ccccggtgat cacggcagag
     2941 ccgagactga tcacgccgat catcgtgagc accatcgaga tgcagaacaa cgcgacgccg
     3001 agcacgatcg cggtccagaa cgtggtggcc tgccatcgga agtgggattc gaggaacgag
     3061 ccgcgcacag cgtcgcggcg cgcgtaattc atgatgatcg cgaggatgga cggcagcccg
     3121 aacacaaagg caccgacgat cgatgcgccc gacgtgacgc cgatgaagcc cgcgagcgcg
     3181 tgcagcgcgt acatgacatg ggtgtagccg gtcaaagggt gctcgttcat ttgggtcgcc
     3241 attccactac cgccacggtg agacgcgaga tacaaaccag tcgttcccgg ggatcgcgga
     3301 tttctatgct ccagacctga ctgcgcgaac ccaggtggaa tgggcgcgcc gtcccgatga
     3361 cgagtccgtc cgacacagga cgcagatggt tggcgttgat ctcctgcccc aggcagcgga
     3421 acttcgcgaa gtcgacgacg aaattggcag cgacgctgcc gagtgtttcc gccaatacca
     3481 gcgacgcacc gccgtgcagg tagcccatgg gctgacgcgt gcgtggctcc accggcatcg
     3541 tgccgcgcag gaaatcgtcg ccccactcgg tgaactcgat gcccaggtgc ggcgcgagaa
     3601 gtcccacctg gctggcgcga gcgtcgtcga tcgtgggatt gccgaaccag attgccaacc
     3661 taactcctcg acgctacttg cgatatttct cgccgatgcg gcgatatgcc tcgcggaacg
     3721 ggatgccttc gcgcgcgacc agcgcattcg cttcctcggc ggcgtgaatg gcggggtcga
     3781 gtgtgatctg gtcggaacgg aaacgcacgc catccatggc gatcgacatg atggcggctg
     3841 tcgacagagc gaggtcgatg cctctgaaca agggcggctt gatgagctga agatcgcgct
     3901 gatatcccga gggcagcttc gcgaagattc cgagcacttc gaacagacac gcctgggcga
     3961 cggcgctgcg tccgcgcacc agctcgaaca cgtcgggatt gcgcttctgc ggcatgatcg
     4021 acgaaccggt ggtgaacgac gcgggcagcg aaacgaacgc gaattcctgc gtgtagaaca
     4081 gcaggagatc cgccgcgagc cgtcccaggt cctgcatgag caacgtgcat tcgaacagca
     4141 gttgcgattc ggccttgccg cgcgcgagct gcacggcggt gaccggctga tgcacttccg
     4201 cgaagccgag cctggcgcgc gtcaactcgc gatcgagcgg caaacccggc gtgccgtacc
     4261 cggcggccga accgagcggg ctgcgcgagg cgcgccgcga aatggacgcc aggccttgcg
     4321 catcgtcgcg cagttccgcg gcgaagccct gggcccacag cgccaccgaa ctcggcatgg
     4381 cctgctgcat atgcgtgtag ccgggcagga cgatggcgcc gtcgcgcgcc gccaggccat
     4441 cgagcgcggc ggcacagcct tccgcggccg cggccagcac gcggcccgcg tcgagtagat
     4501 agaggcggac cgcggtcagc acctggtcgt tgcgcgagcg cccgagatgc acgcggccac
     4561 cggcggcgcc gatgcgcgcc gtgagacgtt tctcgagcgc ggtctggccg tcttcgtcgg
     4621 cgagttcgat cttccattgg ccggcagcat gttcggcgcc gatggcggta agcccgtcgc
     4681 gaatcgccgc aagatccgca tcgctcagca gcttctgcgc gttgagcatc tcggcatgcg
     4741 cgatggaggc gcgcacgtcg tattcgacca ggcgctcgtc gagcgcgtag tcgtcgcccg
     4801 cggtgtacgc gagcacccgc tggtcgagat cgacgccttt gtcccagagc cggctcatgt
     4861 cgttgcttac ttgtcgacct gctgctcggc cggcgacaac gcgttgacgt cggcgacgat
     4921 cagggtgccg gcgccttcgt tggtgaagac ctcggcgagg atgccttcgg gcgacttgta
     4981 cgacatcacg tgcacgcggc gcacgccgcc ccggatggcg tcttcgatcg ccttggcttt
     5041 cggcaacatg ccgtcgacga tcttcttttc ctcgcgcagc cgcttcaggc cgttgaggtc
     5101 cgtgtacgaa atgatggacg tcgggtcgtc gacgcgctcg agaatgcccg gcgcgccggt
     5161 gcacaacatc agcttctccg ccgacaacgc ggcgccgatg gcggccgcga cggtgtcggc
     5221 gttgatgttg agcagcgtgc cgttttcatc ggccgacagc gggctcacga ccggcatcag
     5281 gccattgtcg agcagcttgc ggatgaccgt ggtgtcgatc gagtcgatat cgccgacgaa
     5341 gccgtagtcg accgtctcgc cgctgtcggc gagtctgacg ggcggacgct tgtgggcctt
     5401 gatgagtccc gcgtccacgc cgctgatgcc cacggcgtcg atgtccatct cgcggcagaa
     5461 cgcgagcaga cgggtgttga tgaggccgtt gagcaccatg gaagtggcgt cgatggcctt
     5521 ctggtcggtg acacggcggc cctggaccat gcgcgtgggc acgccgagcg cttcggtgat
     5581 ttccgtgagc tgcgggccac cgccgtgcac gaacaccacg cgaacgccga agtaatggag
     5641 gatcgcgatc tgctcgatga ggccgcgcac ggccgccgga tcggcaaaca cgccgccgcc
     5701 ggccttcacg acgaaggttt tgcccttgta catgcggata tagggcgccg cgctgcgcag
     5761 gctgcggatg gcggcggaag ggtcggggcg atgcatgatc atgcgtgagt tccgttcggg
     5821 cgggagttga gaatagttag tgttgcgggg tcgagagcag gcggtgcagg accgcggtct
     5881 gcacgaccat gcggttgtac gcttcgcgct gcacgacgct gcggcggcca tccagcactt
     5941 catcggcgac ggcgacatta cgccgcaccg gcaggcaatg catgagcttg cagtcgacgg
     6001 cggcgcgcga gaaccaggta tcgcgcacgc accagtcggc gagctgggcc cgctggcgcg
     6061 cgtcggcttc ggcgttgcca tagtgggacg tcgagcccca ctccttggcg tacaccacgt
     6121 gtgcgccgga gagtgcgtcg tcgcgatcgg acgtctcgcg caccgaaccg ccggccgcgg
     6181 cggcggcctg ggccgccttc tgcatgacgg gggcaggcag tgcgaaacct tcgggccgca
     6241 gaacgaccac ttccattccg cgcatggccg ccatgtggac agtggccgcc gggaccgcga
     6301 gcggaagggc gcgcggatgg ttcacccacg acagcacgaa tcgcgctttt cgcggaacgg
     6361 tgagatcgtc gagcgtcttc cagtccgcca atgcctggca gggatggttg gccgccgact
     6421 ccatgttgac gacgggtttg ctgaccagcc cgaccatttc cttgaacgcg gtttcgttca
     6481 gatccgcctc gaggtcggcg ccgcccgcga acgcgcggat gcccagagcg tcacaatagt
     6541 gggccaatac tggcaccccc tcacgtacat gctccgccgg cgcgccattc atgacggcac
     6601 cgcgtcgcgt ctcgagctgc caggttccct ggcccgccga gatgacgaac gagtttccgc
     6661 ccaggcgcgc catggccgcc tgcatcgacg cgagcgtgcg cagcgacggg ttgaagaaca
     6721 gcaggccgag aatcttgccg gcgagcgcat ggggttcggg cttctgcgcc agacgttcgg
     6781 cgagcgcgag cagctcgacg acctgctcgc gcgacatgtc cgccagatcg aggaagttct
     6841 tcatgcgagg tcgatcaacg cggcccgcag ccggtcgacg tgttcctctt tgaggatgaa
     6901 ggcggggagc agtcgaagga tcgagggatc gcccgccgtg cccgcgagga tgtccttggc
     6961 gagcagcgcc ttctggatgt ccttggcggg tttgttcatc ttgagtcccg ccaggacacc
     7021 cgcgccctga aagccgacga ccgggcctac cgcgcaggtc ttcgtgagat acgccgacac
     7081 ccgccgcacg ttggccagca ggtcttcctt ttcgatggcg tcgatggtgg cttcggccac
     7141 ggcgcaggcc atgggtccgc cgccgaacgt ggtgcccagg ttctcgagct tgagcgaggc
     7201 ggtgatgggc ggcgtcagca gcagcgcgga caccgggaag ccgttaccca gcgccttggc
     7261 ggtggtgatc atgtcgggca tcacgccgta atagttagcc gcgaacggct ggcccgtgcg
     7321 gccgacgccg cattgcactt cgtcgaagat gagcagcgcg ccggtctcgt tgcaccggct
     7381 gcgaagtgcc gccaggaact cctggcccat gtcgaatgcg ccgcctacgc cctgcacggg
     7441 ttcgacgatg accgcggccg tgttctcggt gacatggttg gcgatgtcag ccacgtcgcg
     7501 gcgcttgatc cagctcacgt cgaacggcgc gcgcggcagg aagaaccact tttgcggtgc
     7561 ctgccacgtc gtggaagcac aggccgcggt gcggccgtga aagctctgct ccacggcggc
     7621 gacgtgcgtg cggccggggc gcatcttgaa cgccagcttg aacgcgtttt cgttggcctc
     7681 ggcgccggag ttgatccaga acacgctgtc gaacggcagc ttcgaaaacg acagcagctt
     7741 gtgcgcggcg cgcgcgcgga tttccatggg cacggcgttg gtctggaagt tcatctgcgc
     7801 agcctggtcg ctcagcgctt tcgtccagcc cttgtggccg tagccgagcg acgccacggc
     7861 gtggccgccg tacaggtcga gcaccttgcg gccgtcgcgt gtgtgcagcc agacgccgtc
     7921 ggcgccgacc acttcgatcg gatattgcgc atagaccggc gcgagaccat cggccggtga
     7981 ggtgttcttg agttctgcgt tcatgttcgt gttcacgtcc ggccttttca ggtccagccc
     8041 ggagccgggg cggtgagccc cgcggtctcg ggcaggttga acaggcgatt catccattgc
     8101 acggcgccgc cggcggcgcc cttgttgagg ttgtcgacca cgctcatcac ggcgatcgtg
     8161 cgtccgttgg ccacggccgt caggtgcgcg tagttgctgg cgacgatgtc tttcacacgc
     8221 ggagcgttgt cgagcacgcg aacgaacggg cagcccgcgt agtactcgcg cagcatcgca
     8281 acggcggaag ccgcgtcgcg cggtgacttg agcttcgcct gcaccgtggc gtggataccg
     8341 cgcgcgaacg gaccggagtg cggcacgaac gcgaactcgg cttcgacgcc cgatgcggcc
     8401 tttgcgagtg ccgtgatttc gggcgtatgc cgatggccca gcgcgccata ggagtagagg
     8461 tcgctgtggc gcgtcgggtg atgcgtacct tccgtgggct tgcggc
//
LOCUS       DATDEU010000580         5579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_698025, whole genome shotgun sequence.
ACCESSION   DATDEU010000580 DATDEU010000000
VERSION     DATDEU010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698025"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VIV63_13890"
     CDS             complement(<1..327)
                     /locus_tag="VIV63_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125738.1"
                     /translation="MRRFLKWMLRLFVVTVVVAGGLFVWFVAWPVHNIPALEPVDEYV
                     WLDQGWGAGQNAALRQRYYYTAQGTSMPQGASAGAVRYDWFVNLELPLSRDRFAAPEH
                     MRKYRFL"
     gene            488..2146
                     /locus_tag="VIV63_13895"
     CDS             488..2146
                     /locus_tag="VIV63_13895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6125739.1"
                     /translation="MTTIEPGVPVNVEKLSGAISLLEDASGALDIAQVSSAPANEDFI
                     PATPKSANVGFSDSAWWARVTVRNRGDASRFVYLRQDYPLIDTIDLYEPIAGGGWRRH
                     ATGDRTPFGTRDVAHRDFLFPLTVAAGSDATYYLRFSSQGPIDINLSLLDPDTLTAEV
                     SQEQLAYGVYFGCVIMLLVWSGLVFIAVRDGAFLAYFAYIATFGLYMMVNTGLAYQYL
                     WPDSPRWANTCLLVLLSLSLITAVQFSTTILRASDYTPRLNRVARGLQALAVCAIVLS
                     PLLKYSVLVQPITLLIMLSVVFMITLGIVSLLAGSRPARPYVIAWGAFLAGSMVVLLK
                     NFGVLPHTFFTQHSWQVGSLLEMILLSMTLSSRMSELQHQSRTDALTLLGNRRLFNDK
                     LAEEFTRALQQNHPLALLVLDIDKFKHYNDMHGHAQGDEAVKAVAHALRKHARKPYVA
                     CRYGGDEFCAILPGTSEASAAVLAERLRAAVQSSMTGEHTVTVSVGYACHVGTRFENA
                     HQLFEAADAALYSAKEQGRNTIAAFQGRRTGDPPPESADVPSAG"
     gene            2243..2644
                     /locus_tag="VIV63_13900"
     CDS             2243..2644
                     /locus_tag="VIV63_13900"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY6125740.1"
                     /translation="MAEPKLSALELRIMGELWSRGACSIREIHEAVVKRDRPAYTTVQ
                     TMVYRLETKGAVRRTKKIGNAHVFEATTSQASVQTRLLKELLGLFGGNARPVMAHLVE
                     MGKISSEDIDEAQKLLRAHTAKDPLSKDKGK"
     gene            2641..3984
                     /locus_tag="VIV63_13905"
     CDS             2641..3984
                     /locus_tag="VIV63_13905"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY6125741.1"
                     /translation="MISGLINHVWQSTLFCGCAWLITLALRNNSAALRHWIWLLASVK
                     FLVPFSLLFLAGSFIGLPAARIADDQPIVLGDALQNVSVLVSPTSALQAAAPDSGSVA
                     LILLLAVWLGGALLVGTRWLIAWRAADVLVRAARPAPGALSDARITDADIEPAVARVF
                     RPVVLLPAALLARLSRTEIDAVLAHEREHIERHDNLKDSLHRIVETLFWFHPAVWWIG
                     RQMIEERERACDEAVLAYGHDGGEYASGILAVCRHCRASSAQSAAATSGDLTSRVRYI
                     LGNARPSAVGAVKALTLLLATIAATTVPMFAGAVDGTVRRHELLTLNSRVLGTADFSV
                     APAANSAVLRQEIVAGRDGVTIRNTSLRDLIALAYGVERWQVAGNAQWLDGPRYDIRA
                     MAHAPVSEPDELDPYALRDLTAKLLAAHFDLEIHVNRRCQSPCGRYALTTPKPSQ"
     gene            3989..4936
                     /locus_tag="VIV63_13910"
     CDS             3989..4936
                     /locus_tag="VIV63_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125742.1"
                     /translation="MITGLVDHLWQSCCCSGVACAFSWLMRGNAAVARLWLWRIAAIK
                     LLLPFSLLFALGASLGFPSPHAADPAPPTLVRLLAGLAPFASPAESFAWSGFPLALAT
                     LGALAAAAACACWMWNRLLAERARVAEEAARRERNVDDVETRPGFLSAALMSAFVLTA
                     IAIPMLAGAVHDRQQRREWLIANSLALRDAAVDMRPATSGMGNRLRVEANARGVFIRN
                     ASIHDLVAIAYGVNRYSVISPQMMVQGDTASRSWVHWPRYDVHIEAAVPEPRDFEPYA
                     LRQTLTRLLSERFGLEIYVNGDCQPPCGNYHLALADEPL"
     gene            4969..5382
                     /locus_tag="VIV63_13915"
     CDS             4969..5382
                     /locus_tag="VIV63_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125743.1"
                     /translation="MNRRILTGCVLLSFLGATATAMADSQSPARAQLQEFLTAFNSGD
                     RSTIVSFGQDHAPPDFLRAQIVDQTLEMYRESGGYDLLEVNETGPLSLTSWVRARKTR
                     EIVLLAVQVHADEPGRISMIGFHSDAPPERLRTRQ"
BASE COUNT          952 a         1879 c         1722 g         1026 t
ORIGIN      
        1 gaggaagcgg tatttgcgca tgtgttccgg ggcggcaaac cggtcccgcg acaacggcag
       61 ctcgaggttc acgaaccagt cgtaacgcac cgcaccggcc gatgcgccct gcggcatcga
      121 ggttccctgc gcggtgtagt agtagcgctg tcgcagcgca gcgttttgtc cggcgcccca
      181 accctggtcc agccagacgt attcatccac cggttccagc gcggggatgt tgtgcaccgg
      241 ccaggccacg aaccagacaa acaaaccacc cgccaccacg actgtcacga cgaacaggcg
      301 caacatccat ttcagaaaac ggcgcatcgc gttactccct taactttctg acgcatacta
      361 caaactaaca tcccgcacgc aggaagcgat tttgaactgg tccgcaagtg ttgcaagtac
      421 ccgcgtcacg ctgatcgcgt tgctgttagt tagctgccta ccaatcccgg ctgccgggga
      481 aactcgggtg accacgatcg agccaggcgt tcccgtcaac gtcgagaagc tgagcggtgc
      541 gatctccctg ctcgaagacg ccagcggcgc tctcgacatc gcccaggtga gcagcgctcc
      601 ggcgaacgaa gatttcatcc ccgccacgcc caagtcggcc aacgtgggct tttcggattc
      661 ggcatggtgg gcgcgggtca ccgtgcgcaa tcggggcgac gcgagccggt tcgtctatct
      721 gcgccaggac tatccgctca tcgatacgat cgacctgtac gagccgatcg cgggcggcgg
      781 ctggcgcaga catgccaccg gcgaccgcac gcccttcggc acccgcgacg tcgcgcatcg
      841 cgatttcctg tttccgctga cggtagccgc cggcagcgac gctacttact acctgcgttt
      901 cagctcccag ggtccgatcg acatcaacct gtcgctgctc gacccggaca cgctgaccgc
      961 ggaggtgagc caggagcaac tcgcgtacgg cgtgtacttc ggctgcgtga tcatgctgct
     1021 ggtgtggagc ggcctggtgt tcatcgcggt tcgcgacggc gcgtttctcg cgtacttcgc
     1081 gtacatcgcc accttcggcc tctacatgat ggtgaacacg ggcctcgcct atcaatatct
     1141 ctggcccgac agcccccggt gggccaacac ctgcctgctc gtgttgttga gcctctcgct
     1201 catcacggcg gtgcagttct ccaccacgat cctgcgcgcc agcgactaca cgccgcggct
     1261 caaccgggtc gcgcgcgggc tgcaggcact ggcggtgtgc gccatcgtgc tgagcccgct
     1321 gctcaagtac tcggtgctgg tgcaacccat cacgctcctc atcatgttgt ccgtggtctt
     1381 catgatcacg ctcggcatcg tgagcctgct cgccggttcc cgcccggcgc ggccctatgt
     1441 cattgcgtgg ggcgcattcc tcgcgggatc catggtggtc ctgctcaaga acttcggcgt
     1501 gctgccgcac acgttcttca cgcagcacag ctggcaggtg ggctcgctgc tcgagatgat
     1561 cctgctgtcg atgacactgt cgagccgcat gagcgagctg cagcaccaga gccgtacgga
     1621 cgcgctcacg ctgctcggca accgccggct gttcaacgac aaactagccg aagaattcac
     1681 gcgggcgctt caacagaacc atccgctcgc cctgctcgtg ctcgatatcg acaagttcaa
     1741 gcactacaac gacatgcatg gccatgcgca gggagacgaa gccgtgaaag ccgtggcgca
     1801 tgcgctgcgc aaacacgcgc gcaagcccta tgtcgcgtgc cgttacggcg gtgacgagtt
     1861 ctgcgccatt ctgccgggta cgtcggaagc atcggccgcg gttctggccg aacggctgcg
     1921 cgcggccgtg cagagttcta tgactggcga acacaccgtc acggtcagcg tcgggtacgc
     1981 gtgtcacgtc ggcacgcggt tcgaaaacgc gcaccaattg ttcgaagccg ccgatgcggc
     2041 gctttattcc gcgaaggaac aggggcgaaa taccatcgcg gcattccagg gccgccgaac
     2101 cggcgatcca ccgcccgaat ccgccgacgt tcccagcgcc ggttgaactt gacggcgggc
     2161 cttcaaatgc ccgctcgcag gcacttgcgt tcgctactac acctgtcata gagttgaccg
     2221 cagttgtagt tatggaggtc tcgtggccga accgaagctg agcgcgctgg agctgcgcat
     2281 catgggcgag ctgtggagtc gcggcgcctg ttccattcgc gagatccacg aagccgtggt
     2341 caaacgcgac cggcccgcct acacgaccgt gcaaaccatg gtttaccggc tcgagaccaa
     2401 gggcgcggtg cgccgcacca agaagattgg caacgctcac gtgttcgaag ccaccacttc
     2461 gcaggcttca gtgcagacgc gcctgctcaa ggaattgctc ggattgtttg gcggcaatgc
     2521 gcgccccgtc atggcgcacc tggtcgagat gggcaagata tccagcgagg acatcgacga
     2581 agcgcagaag ctgctgcgcg cgcataccgc gaaggaccct ctctcgaagg acaagggcaa
     2641 atgatttccg gcctgatcaa ccatgtctgg cagtcgacgc tgttctgcgg ctgcgcctgg
     2701 ttgatcacgc tcgcgttgcg caacaacagc gcggcgctgc gccactggat ctggctgctc
     2761 gcctcggtca aattcctggt gcccttttca ttgttgtttc tggcgggctc gttcatcgga
     2821 ctgccggcag cgcgcatcgc cgacgatcag cccatcgtgc tcggcgacgc actgcaaaac
     2881 gtctccgtgc tcgtctcgcc gacgagcgcg ctgcaggcgg ccgcaccgga cagcggttcc
     2941 gtcgcgctca ttttattgct ggccgtctgg ctcggcggcg cattgctcgt cggcacacgc
     3001 tggctgatcg cgtggcgcgc ggccgatgta ctggtaaggg cggcgcggcc ggcgccgggc
     3061 gcactatcgg atgcgcgcat cacggatgcc gatattgaac cggccgtggc gcgcgttttt
     3121 cgtccggtgg tgttgctgcc ggccgcgttg ctcgcgcggc tctcgcgaac ggagatagat
     3181 gccgtgctcg cgcacgagcg cgaacacatc gaacggcacg acaacctcaa ggacagcctc
     3241 catcgcatcg tcgaaacgct gttctggttt catccggcgg tgtggtggat cggccgccag
     3301 atgatcgaag aacgcgaacg cgcctgtgat gaagccgtcc tggcgtatgg ccacgacggt
     3361 ggcgaatacg cttccggcat tctcgcggtg tgccgtcatt gccgcgcgag ctcggcgcaa
     3421 tcggcggcgg cgacttccgg cgatctgacg agtcgcgtgc gatatatcct cgggaatgcg
     3481 cgcccctcgg ccgtcggcgc cgtcaaggcg ctgaccttgc tactggccac catcgccgcc
     3541 accaccgtgc cgatgttcgc gggcgccgtc gatggcacgg ttcgccggca cgaactgctt
     3601 accctcaatt cgcgcgttct cggaaccgcg gatttttcgg ttgctcccgc ggcgaacagc
     3661 gccgtccttc gccaggaaat cgtcgccgga cgcgatggcg tcaccatccg caacacatcg
     3721 ctgcgcgacc tgatcgcgct cgcctacggc gtcgagcgtt ggcaggtggc gggcaacgcc
     3781 caatggctgg acggaccgcg ctatgacatc cgcgccatgg cgcatgcgcc cgtgagtgaa
     3841 cccgacgaac tcgatccata cgcgctgcgc gatctgaccg ccaaactact cgccgcgcat
     3901 ttcgatctgg agattcacgt caaccgccgc tgtcagtcgc cttgcgggcg atacgcgttg
     3961 acgacgccca aaccctcgca gtagctccat gatcacgggg ctggtcgatc atctgtggca
     4021 atcctgctgc tgctctggag tcgcgtgcgc gttctcgtgg ctcatgcgcg gcaacgcggc
     4081 ggtggcgcgc ctatggctgt ggcgaatcgc ggcgatcaaa ctgctgctgc cgttttcgct
     4141 gttgttcgcg ctgggtgcat cgctgggatt tccgtcgcca cacgccgcag atcccgcacc
     4201 gcccacgctc gttcgactgc tcgcaggact cgcgccgttc gcgtcgcccg cagaatcgtt
     4261 tgcctggagc ggttttccgc tcgccctcgc gacactcggt gcgctcgccg cggcggcggc
     4321 ctgcgcctgc tggatgtgga accggctgct cgccgagcgt gcccgggttg ctgaagaagc
     4381 ggcccgtcgt gagcgcaacg tcgacgacgt cgagactcgt ccgggatttc tcagtgccgc
     4441 gttgatgtcg gcattcgtgt tgactgcgat cgccatcccg atgctcgcgg gcgcggttca
     4501 cgaccggcag cagcggcgag agtggctgat cgccaattca ctcgcgctgc gtgacgctgc
     4561 cgtcgacatg aggccggcca ccagcggcat gggcaaccgc cttcgcgtcg aagcgaacgc
     4621 gcgcggcgtc ttcatccgca acgcgagcat ccatgacctc gtggccatcg cgtatggcgt
     4681 gaaccggtat tcggtcatca gcccgcagat gatggtgcag ggagataccg ccagccgatc
     4741 gtgggtccat tggccgcgat acgacgttca catcgaggcg gcggtacccg agcctcgtga
     4801 cttcgaaccc tacgcgctgc gccagaccct cacgcggctg ctctccgaaa gattcggcct
     4861 cgagatctac gtgaatggcg attgccaacc gccttgcggc aactaccacc tcgcgctggc
     4921 cgacgagcct ctctagacgc gtgacgaact accataggag aacatgtcat gaaccgacga
     4981 atcttgacgg gatgtgtatt gctgagtttt ctgggcgcga ccgcaaccgc aatggccgac
     5041 tcgcagtccc cggcgcgagc gcagctccag gaatttctga cggcgttcaa ttccggagac
     5101 cggtcgacga tcgtctcgtt tggacaagat cacgcgccac cggatttcct gcgtgcgcag
     5161 atcgtcgacc agacgctgga gatgtaccgg gaatcgggcg gctacgatct gctcgaggtc
     5221 aacgaaaccg gaccgctctc gctgacgagt tgggtacggg cgcggaaaac ccgcgagatc
     5281 gtgttgctcg ccgtccaggt ccacgccgac gagcccggac ggatcagcat gatcgggttt
     5341 cactccgacg ccccgcctga acgcctgcgc acgcgccagt aaccgcaggc agttcgcgtc
     5401 gcggccatcg atccgatcga gatggccacg acgcgcgccg tcgcacctcg cgcagctgaa
     5461 ccgcgccggc ttccctagct accggccagc agctcgagct gcccgcgccg cgccacgcgc
     5521 ctgtcgaaca ggccccaggc accctcgccc ccgtgccacg cgccgcccgt cgctgcctt
//
LOCUS       DATDEU010000581         8336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_404605, whole genome shotgun sequence.
ACCESSION   DATDEU010000581 DATDEU010000000
VERSION     DATDEU010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404605"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VIV63_13920"
     CDS             <1..1076
                     /locus_tag="VIV63_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY6125744.1"
                     /translation="LKDEWHASPKIQARMKELGVKDEHALQSYFIQRIGKFIDSKGRK
                     LIGWDEILEGGLAPNATVMSWRGIDGAIAAAKAGHDTVLSPAPDLYLDHWQSPGDTSP
                     GRSNTLSLKDVYLFNPLPESILPEQRKHVLGLQGNLWAEMMRSEARVTYMAYPRAAAL
                     AEVAWSPPERINWEEFEKRLEPHLERYATLGIGYGREVKVEPGPRRRVSHDLDQCGGG
                     YVLSLEDDAPVGGERATFLVNITDPCWIWKGVDLSQVKAIRATVGQIPFNFQIGADAA
                     KIPLIKPATKYGELQIRIDDCKAGQVVASQSLEPAVSNHGLTPLPSIDLPSEQGTHDI
                     CFLFTRAKIDPIWVIGGIELVGF"
     gene            1079..3559
                     /gene="ptsP"
                     /locus_tag="VIV63_13925"
     CDS             1079..3559
                     /gene="ptsP"
                     /locus_tag="VIV63_13925"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY6125745.1"
                     /translation="MPLTIRAPFAGWCAPLSEIPDAAFAQGMLGDGVGLDPTGNELRS
                     PCDGEVISVAASRHALALRTATGAEILVHVGVDTVGLGGEGFNVHVRKGDKVRAGDLL
                     LTFDLDLLAHRAPSLMTPIIVTNADRFRISQANTRRVVASGDVLFEIEQFAAGDAGDV
                     AEPGAASVAVVSEAVVVEHAHGIHARPAALIARIAKALPYEIELRARGRGARARSTVA
                     LMSLGIRGGDEVVIAGFEPAAADGIAAIARAIRNLETVAAHAEVPAPTATAASSSDPA
                     NLRGVTANRGLAIGPAFYLQAADLQVAETGSGIGEETLAFDRARDSVRARLSRLAATA
                     APTVREIMSAHLELLDDPDLVDAARAGIATGSSAGFAWRASLRASAKSLRDTGDARLA
                     ERVDDLRDLESQVLQALEGAEAPAFDIPPGSILLARDLTPSQLVDIDTRQLAGIALGA
                     GGPTSHVAILAATLGIPMVVAVGPALLDVAAGSTIILDADAGAVRAGLDAAALAEARQ
                     RLDRSRAQRAQQLASAQDSCHLASGERIEVFANLTGSETDAHSAVTQGAEGCGLLRTE
                     FLFLERETAPDENEQLRCYQRVATILGPRPLIIRTLDIGGDKPIPYLPLPPEDNPALG
                     LRGVRTSLWRPDLLAVQLRALLQVQPAGQVRILLPMVTDVAEIRAVRRLLDDACSALG
                     VTQPALGAMIETPAAAMLADGIAREVDFLSIGTNDLAQYTLAMDRGHAELAARIDGVH
                     PAVLRMIDFACRGAAAQRKPIAVCGGLAADAAAVPVLLGLGVTELSVVPSLIPQLKAQ
                     VRALTLDSCRKIAARALALESAEQVRAS"
     gene            3556..5313
                     /gene="nagE"
                     /locus_tag="VIV63_13930"
     CDS             3556..5313
                     /gene="nagE"
                     /locus_tag="VIV63_13930"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR01998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HEY6125746.1"
                     /translation="MNKIISSLQPIGRALMLPIAVLPVAALLLRLGQPDLLDVPAIAA
                     AGDAIFSNLGLLFAIGVAVGLARENHGAAGLAAVVGYLVATQGAKVLIDVPPEVTANV
                     PDQLKELAAAAFRSKELSKLSVPVGLLSGLIAGWAYNRFSDIRLPSYLAFFGGRRFVP
                     IVSGFAGLGLALIFGFGWQTLESGMDATSQAVLGSESIGLFAYGVLNRLLIVTGLHHI
                     INNIAWFLLGDYNGVTGDLKRFFAGDPSAGAFMSGFFPVMMFGLPGACLAMYRTATPE
                     RRASAGGLLLSMALTSFLTGVTEPIEFAFMFLAPVLYAIHAVLTGLSMVLMHALDVHL
                     GFGFSAGLFDYVLNYSLSTRPLMLIPIGLGYFAIYYGLFRYCIVRFNLKTLGREGEGA
                     AVAAVDAGKNSAPALGWIRALGEAGNLRAVEACTTRLRLVLNDPNLIDESALTVLGSR
                     GVLRIGDGAVQVVVGPIADQLASEIRTQWRAPATGGAPALKPGPEMTSASASPDRASI
                     DAALAALGGRANIVKVHANSTRLVIAVRNAGVVDESALGRVVQAVARPNPASLHLIVG
                     PAAKAWAREWANTVETPAA"
     gene            5638..6747
                     /locus_tag="VIV63_13935"
     CDS             5638..6747
                     /locus_tag="VIV63_13935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6125747.1"
                     /translation="MAVSLLSLLGLTGCGKGADGKTPEVEKLAAMQIAPEDVITLSSS
                     ALASGPSITGSIEPERRADLRAEVSAVVLAVLKENGDPVKRGDLIVRLDQTSIRDSLT
                     AAEASARAADQAYEQAERQFQRMSTLKQTGVVSTQQVEDVEIRRNSAQSDRAEARTRM
                     VTARQQLARTEVRAPFDGIVSDRKVSAGDTAAIGKELVKVIDPTSLRFEGLVSADSIG
                     EVKAGQHVWFKIHGFSAEEFTGVVQRVNPAANVTTRQVEVLVAFDDAKKQPNVAGLYA
                     EGHVETRSSAALSLPSASIVNEGDASFAWRVKDAKLQKVTLVLGERDARTGAFALRSG
                     LAEGDKVLRFPNATLKDGQEVQAGGAAKPALVVEK"
     gene            6757..>8336
                     /locus_tag="VIV63_13940"
     CDS             6757..>8336
                     /locus_tag="VIV63_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6125748.1"
                     /translation="MFLSNFSIKQPVTTVAIVIVLMCLGLLALKNLRVNQIPDVENPV
                     MSVSFPYPGASPETVEREIINRVEKPLQSIPQVYEIWSNASDGNANFTIIFDFDKEMT
                     EAGDEIRNAIASVRYKLPIEMREPVLWRRDPSADPIVNLGLSSTKQTHAEISRYAEDV
                     LADQLRGIDGVSVVNVDGALHRELSVLLHAQKMREYNVSAGDVVNALRAQNTNAPVGR
                     VKGTMDEQNIRLVGRIERPAEFNDIVVKRQGGEIVRLGQVATVEDGFAEPGSYSLRSG
                     QPNVGLSIIRSREASTVSVAERVRKEVEEINKTLPEGTKLEITRDGGEEASQNLNNVI
                     HALIFGAGLTIFVVYAFLNSWRSTLITGLSLPTSAVAAFIAIWLCGFTLNFMTLLGLS
                     LAIGVLIDDAIVVRENIVRHMERGEDRITAARNGTAEIGLAVTATTFSIVAVFVPVAF
                     MGGGAGEWFRPFAVTVVSSVLVSLFISFTLDPMLSAYWGDPPGYAHSERRGLGKYFAR
                     FNTWFDTQANGYGKVIEWA"
BASE COUNT         1400 a         2764 c         2752 g         1420 t
ORIGIN      
        1 cgctgaaaga cgaatggcac gcttcgccga agatccaggc ccgcatgaaa gagctcggcg
       61 tgaaggacga acacgcgctg cagagttatt tcatccagcg catcggcaag ttcatcgaca
      121 gcaaggggcg caagctcatc ggctgggatg aaattctcga aggcggtctc gcgccgaacg
      181 ccaccgtcat gtcgtggcgt ggaatcgacg gcgcgatcgc cgccgcgaag gccggccacg
      241 acaccgtgct gtctccggcg cccgatctgt atctcgatca ttggcaaagc cccggcgaca
      301 cctcgcccgg ccgcagcaac acgctgtcgc tgaaagatgt gtacctgttc aacccgctgc
      361 ccgaatccat tcttcccgag cagcgcaaac atgttctcgg tctgcaggga aatctctggg
      421 cggaaatgat gcgctccgag gcgcgcgtga cgtacatggc gtatccacgc gccgcggcgc
      481 tggccgaagt ggcctggtcg ccgccggaac gcatcaactg ggaggagttc gagaagcgtc
      541 tcgaaccgca cctcgagcgc tacgccacgc tcggcatcgg ttatggccgc gaggtgaagg
      601 tggagccggg tccgcgccgc cgcgtgagcc acgatctcga tcagtgtggc ggcggctatg
      661 tgctgtcgct cgaagacgat gcgccggtcg gcggcgaacg cgcaacgttc ctcgtgaaca
      721 tcaccgatcc gtgctggatc tggaaagggg tggatctgtc gcaggtgaaa gccatccgcg
      781 ccaccgtcgg gcagattccc ttcaacttcc agatcggcgc cgatgccgcg aagatcccgc
      841 tcatcaagcc cgcgacgaaa tacggcgagc tccagatccg catcgacgac tgcaaggcgg
      901 gccaggtagt ggccagccag tcgctggaac ccgcggtgtc caatcacggg ctgacgccct
      961 tgcccagcat cgacttgccg tcggagcagg gtacacacga catctgcttc ctgttcacgc
     1021 gcgcgaagat cgatcccatc tgggtcatcg gcggcatcga actcgtcgga ttctgaggat
     1081 gccgctgacc atccgcgctc cgttcgccgg ctggtgcgcg ccgctctcgg agattcctga
     1141 cgcggccttc gcgcaaggca tgctcggcga cggcgtggga ctcgatccca cgggtaatga
     1201 gctgcgctcg ccgtgtgacg gcgaggtgat ctccgtcgct gcgtctcgcc acgcgctggc
     1261 gttgcgcacc gccaccggcg cggaaattct cgtacacgtg ggtgtcgaca cggtgggtct
     1321 cggcggcgaa ggcttcaacg tgcacgtgcg caagggcgac aaggtccgcg ccggtgatct
     1381 gctgctcacc ttcgacctcg atctgctcgc gcaccgcgcg cccagtctca tgacgccgat
     1441 catcgtcacg aacgcggacc gctttcgtat ctcgcaggcc aacacgcggc gagtggtcgc
     1501 gagcggcgac gtgttgttcg agatcgagca gttcgccgcc ggagatgcgg gcgatgtcgc
     1561 cgagcccggc gcggcaagcg tcgcggttgt cagcgaagcg gtggtggtcg aacacgccca
     1621 cggcatccac gcgcggccgg cggcgctcat cgcgcgcatc gccaaggcct tgccttacga
     1681 aatcgagttg cgtgcgcgag gccgcggtgc acgcgctcgc agcaccgtgg cgctgatgtc
     1741 gctcggtatt cgcggcggcg acgaagtcgt catcgccgga ttcgagccgg ccgccgccga
     1801 tggaatcgcg gcgatcgcga gggccatccg gaatctcgaa acggtggcgg cgcacgcgga
     1861 agtcccggcg ccgacggcaa ccgccgcgtc cagcagcgac cccgcaaatc tgcgcggcgt
     1921 caccgccaat cgcggtctcg ccatcgggcc ggcgttttat ctgcaggcgg ccgatctgca
     1981 ggtcgccgaa accggctccg gcatcggcga agaaacgctg gcgttcgatc gcgcgcgcga
     2041 ttccgtgcgc gcacgtctgt cgcggctcgc cgccacggcc gcgcccaccg tgcgcgagat
     2101 catgtccgcg cacctggaat tgctcgacga ccccgatctg gtcgatgccg cgcgcgcggg
     2161 aatagccacg ggctcgagcg cgggcttcgc gtggcgcgcc agtctgcgcg ccagcgcgaa
     2221 gagcctgcgc gacacgggcg atgcgcgtct cgccgaaagg gtcgacgatc tccgggatct
     2281 ggaatcgcag gtgttgcagg cactcgaggg cgccgaggcg ccggccttcg acattccacc
     2341 gggcagcatc ctgctcgcgc gggacctcac gccttcacag ctcgtcgaca tcgacacgcg
     2401 gcaactcgcc ggcatcgcgc tcggcgcggg aggtcccact tcgcacgtcg ccatcctcgc
     2461 cgcgacgctg ggcattccca tggtggttgc cgtgggcccc gcgctgctcg atgtggccgc
     2521 cggcagcacg atcattctcg atgccgacgc gggcgcggtg cgcgccggtc tcgatgcagc
     2581 agcgcttgcc gaagctcgcc agcggctcga tcgcagccgt gcgcagcgcg cgcaacagct
     2641 cgcgtccgcg caggacagct gccatctcgc cagtggcgaa cgcatcgagg tatttgccaa
     2701 cctcacgggt tcggaaacgg atgcccactc ggcggtgacg cagggcgccg aaggctgcgg
     2761 cctgttgcgc accgagttcc tgttcctcga gcgggagacg gcgcccgatg agaacgaaca
     2821 attgcgttgc taccagcggg tggcgacgat cctgggcccg cgtccgctga tcatccgcac
     2881 gctcgacatc ggcggcgaca agcccattcc ctatctaccg ctgccgcccg aggacaatcc
     2941 ggcgctgggc ctgcgcggcg tgcgcaccag cctgtggcgt ccggacctgc tcgccgtgca
     3001 gttgcgcgcg ttgctccagg tgcagccggc ggggcaggtg cgcatcctgt tgccgatggt
     3061 gaccgacgta gccgagatcc gcgccgtgcg ccgtctgctc gacgatgcct gctcggcact
     3121 cggggtcacg cagcctgcgc tcggcgccat gatcgaaacg ccggccgcgg cgatgctcgc
     3181 ggatggcatc gcgcgcgaag tcgattttct gtcgatcggc accaacgatc tcgcgcaata
     3241 cacgctggcc atggatcgcg gccacgccga gctggccgcg cgcatcgacg gcgttcatcc
     3301 ggcagtcctt cgcatgatcg acttcgcctg ccggggagcg gcggcgcagc gcaaacccat
     3361 cgccgtgtgc ggaggcctgg ccgcggatgc ggcggcggtg ccggtgttgc tgggcctcgg
     3421 cgtcacagaa ttgtccgtgg tgccttcgct cattccccag ctcaaggcgc aggttcgcgc
     3481 gctgacgctg gattcctgcc ggaagattgc cgcgcgggcg ctcgcgctcg aaagcgcgga
     3541 acaggtgcgc gcgtcgtgaa caagatcatc tcgagtctgc agcccatcgg ccgcgcgctg
     3601 atgttgccca tcgcggtgct gccggtggcg gcgctgctgt tgcgcctggg gcagccggat
     3661 ttgctggatg ttccggccat cgccgccgcg ggcgacgcga tcttctcgaa cctcggactc
     3721 ctcttcgcca tcggcgtcgc cgtcgggctc gcgcgcgaga atcacggcgc ggccgggctc
     3781 gccgccgtgg tgggttatct ggtcgccacg cagggcgcga aggttctcat cgatgtgccg
     3841 ccggaagtca cggcgaatgt tcccgatcaa ttgaaggaac tcgccgccgc ggctttccgc
     3901 agcaaggagc tctccaagct cagcgtgccc gtgggactgt tgtcgggatt gatcgccggc
     3961 tgggcttaca accgtttcag cgacatccgg ctgcctagct acctggcatt cttcggcgga
     4021 cggcgcttcg tccccatcgt cagtggattc gcggggctcg gccttgcgct catcttcggc
     4081 ttcggctggc agacgctcga gagcggcatg gatgcgacga gccaggcggt gctcgggtcc
     4141 gaatccatcg ggctcttcgc ctatggcgtg ctgaaccgcc tgctcatcgt caccggcctg
     4201 caccacatca tcaacaacat cgcctggttc ctgttaggcg actacaacgg cgtcaccggc
     4261 gatctgaaac gcttcttcgc gggcgaccct tccgcgggcg cgttcatgtc gggattcttc
     4321 cccgtcatga tgttcggcct gccgggagcc tgtctcgcca tgtaccgcac cgcgaccccg
     4381 gaacggcgcg cgtcggccgg cggcctgctg ctgtcgatgg cgctcacgtc gttcctcacc
     4441 ggagtcaccg agcccatcga gtttgcgttc atgttcctgg cgcccgtgct ctatgccatc
     4501 cacgcggtgc tcaccggact ttcgatggtg ctcatgcacg cgctcgatgt gcatctcggg
     4561 ttcggtttct ccgcgggcct gttcgactac gtgctcaatt attcgctctc gacccggccc
     4621 ctgatgctca tccccatcgg cctcggttac ttcgccatct attacggcct gttccggtac
     4681 tgcatcgtgc gtttcaacct gaagacgctc ggccgcgagg gcgaaggcgc cgcggtggcg
     4741 gcggtcgatg ccggcaagaa ctcggcgccg gcgctcggct ggatccgcgc actgggcgag
     4801 gccggcaatt tgcgcgcagt cgaggcctgc accacgcggc tgcggctggt gctgaacgat
     4861 ccgaatctca tcgacgaatc ggcgctcacg gtcctgggtt cgcgcggcgt gctgcgaatc
     4921 ggggacggcg ccgtgcaggt ggtcgtgggt cccatcgcgg atcagctcgc ctcggaaatc
     4981 cgcacgcagt ggcgcgcgcc ggcaaccggg ggcgcaccgg ccttgaaacc gggacctgag
     5041 atgacctcgg cgtcggcttc cccggaccgt gcctcgatcg acgccgctct cgccgcgctc
     5101 ggcggccggg caaacatcgt gaaagtgcac gccaattcca cgcgcctcgt gatcgcggtg
     5161 cgcaatgcgg gcgtggtgga cgagtccgcg ctcgggcgcg tggtccaggc agttgcacga
     5221 ccgaacccgg cgagcctcca tttgatcgtc ggtccggcgg ccaaggcctg ggccagggaa
     5281 tgggccaata ccgtggaaac cccggcagcc tgatcgcaga tcgggcggct gccgattcct
     5341 ggcactgaat ccgggcataa cggccgtccg gtttcgcagg atgcgccgag gcgatgtcaa
     5401 aaagcggcgc gccatgacaa ctcacacttt caaacgaatc agaggcttac taaactgccc
     5461 gccttccgaa acgggaattg cgggccgatc cggcggctga cgcatgaatt cccaacagaa
     5521 acaacacatt tagttagggg cggtttgcaa cccgaagggt tgttcgaacg tcgtatgtcg
     5581 gggcccccga aggaatattg cgtgaccaaa agaatcagaa ctctccgcca cgttggtttg
     5641 gcggtctccc tgctttcgct gctcggtctc accggctgcg gcaaaggcgc cgacggcaag
     5701 acgcccgagg tggaaaagct ggcggcgatg cagatcgcgc ccgaagacgt catcacgctg
     5761 agcagcagtg cgctcgcttc cggaccgtcc atcacggggt ccatcgaacc cgagcgtcgc
     5821 gcggatctgc gcgccgaagt gtcggccgtg gtgctcgccg tgctcaagga gaacggtgat
     5881 ccggtcaaac ggggcgacct catcgtgcgg ctcgaccaga cctcgatccg cgacagcctc
     5941 accgccgccg aagcatcggc gcgcgccgcc gatcaggcct acgaacaagc cgagcgtcag
     6001 ttccagcgca tgagcacgtt gaagcagacc ggtgtcgtat cgacgcagca ggtcgaagac
     6061 gtggaaatcc gccgcaactc cgcgcagagc gatcgcgccg aggcgcgtac gcgcatggtc
     6121 acggcacgcc agcagctggc gcgcaccgaa gtgcgcgcgc cgttcgacgg catcgtcagc
     6181 gaccgcaagg tgtcggcggg cgacaccgcg gccatcggca aggaactggt caaggtcatc
     6241 gacccgacga gcctgcgctt cgaaggcctg gtgtcggccg acagcatcgg cgaagtgaaa
     6301 gccgggcagc acgtgtggtt caagatccat ggcttctccg cggaagaatt cacgggcgtc
     6361 gtccagcgag tgaatccggc ggcgaacgtg acgacgcgcc aggtcgaagt gctcgtggca
     6421 ttcgatgacg cgaagaagca gccgaacgtc gcgggcctgt acgccgaagg gcacgtcgag
     6481 actcgcagca gcgcggcatt gagtcttccc agcgcatcca tcgtgaacga aggcgacgcc
     6541 tcgtttgcgt ggcgcgtgaa ggatgcgaag cttcagaaag tgacactcgt cctcggtgag
     6601 cgcgatgcgc gcacaggcgc attcgcgttg cgcagcggtc ttgccgaagg cgacaaggtg
     6661 ctcaggtttc cgaacgccac gttgaaggac ggccaggaag tgcaggccgg cggcgcggcg
     6721 aagcccgcgc tggtcgtcga gaaataagga aaagccatgt tcctttccaa tttcagcatc
     6781 aagcagccgg tgacgacggt cgcgatcgtc atcgtgctca tgtgtctcgg gttgctggcg
     6841 ctgaagaacc tgcgcgtcaa ccagatcccc gacgttgaga acccggtgat gtcggtcagc
     6901 ttcccgtatc ccggcgcctc gccggaaacg gtggagcgcg aaatcatcaa ccgcgtcgag
     6961 aagccgctgc agagcattcc gcaggtctac gagatctggt cgaacgcgtc ggacggcaac
     7021 gccaacttca ccatcatctt cgacttcgac aaggagatga ccgaggccgg tgacgagatc
     7081 cgcaacgcga tcgcgtcggt gcgttacaag ctgccgatcg aaatgcgcga gccggtgctg
     7141 tggcggcgcg atccgtcggc ggatccgatc gtcaacctgg gcctgtcgtc gaccaaacaa
     7201 acgcatgccg agatctcccg gtacgccgag gacgtgctcg ccgaccagct gcgcggcatc
     7261 gacggcgttt ccgtggtcaa cgtggacggc gcgctgcatc gcgagctgtc ggtgctgctg
     7321 cacgcgcaga agatgcgcga gtacaacgtg tccgccggcg acgtcgtgaa cgcgctgcgc
     7381 gcccagaaca ccaatgcacc cgtcggccgc gtcaagggca cgatggacga gcagaacatc
     7441 cggctcgtgg gccgcatcga acgcccggcc gagttcaacg acatcgtcgt caaacgccag
     7501 ggcggcgaga tcgtgcggct gggccaggtg gccacggtcg aagacggttt cgcggagccg
     7561 ggcagctact cgttgcgcag cggccagccc aacgtgggtc tgtccatcat ccgctcgcgc
     7621 gaggccagca cggtctcggt cgccgagcgc gtgcgcaagg aagtcgagga gatcaacaag
     7681 acgctgcccg agggcacgaa gctcgagatc acgcgcgacg gtggcgaaga agcctcgcag
     7741 aacctcaaca acgtgatcca cgcgctgatc ttcggcgccg gcctcacgat cttcgtggtg
     7801 tacgcgttcc tgaattcctg gcggtcgacg ctcatcaccg gcctgtcgct gccgacttcg
     7861 gccgtcgcgg cattcatcgc catctggctc tgcggcttca cgctgaactt catgacgttg
     7921 ttaggcctgt cgctggccat cggcgtgctc atcgacgacg ccatcgtggt gcgcgaaaac
     7981 atagtccggc acatggaacg cggggaagac cgcattaccg ccgcgcgcaa cggcaccgcg
     8041 gagatcggtc tcgcggtgac cgccaccacg ttttcgatcg tcgcggtgtt cgtgcccgtg
     8101 gcattcatgg gcggtggcgc gggtgaatgg ttccggccat tcgcagtcac ggtcgtgtct
     8161 tcggtattgg tcagtctctt catctcgttc acgctcgacc cgatgctctc ggcctactgg
     8221 ggtgacccgc cgggctatgc gcactccgaa cgccgcgggc ttggcaaata tttcgcgcga
     8281 ttcaacacct ggttcgacac ccaggcgaat ggctacggca aggtcatcga gtgggc
//
LOCUS       DATDEU010000582         3248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1864670, whole genome shotgun sequence.
ACCESSION   DATDEU010000582 DATDEU010000000
VERSION     DATDEU010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864670"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VIV63_13945"
     CDS             <1..744
                     /locus_tag="VIV63_13945"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEY6125749.1"
                     /translation="LVGNRQADIRTSVENEWLCARIVAAFGLPVARCEIVQVDDQKVL
                     IVERFDRVLHSSGKYWLRLMQEDFCQATGTPWHRKYQADGGPGLAEMARILRGSVESQ
                     EDLRTVFRTQLLFWMLAATDGHAKNYSIRILQGGRFQLTPLYDVLSAWPIIGKRASEI
                     PLEKVKMAMAMPGQRPHYLHKSIQRRHFETLAKRLGVEADAEKLIAEMTQKAPAIIEE
                     VQRGLPRNFPQGLLDRVLGGLAKSAKAMA"
     gene            complement(741..>3248)
                     /locus_tag="VIV63_13950"
     CDS             complement(741..>3248)
                     /locus_tag="VIV63_13950"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6125750.1"
                     /translation="PESERPALLKREARGDSELIAEVASLLTSHDAPSGLLDAVPAQL
                     KAQAFAVDETSRLGERIGAYRIVGILGTGGMGQVFKAVRDDDQYHAEVAIKLMRADVS
                     GKLADERFRTERQILAGLDHRNIARLIDGGTTAGGSPYVVMELVDGESIDTFADAHSL
                     KIRERVQLFLQVCAAVSYAHQHLVIHRDLKPNNILVTADGSVKLLDFGIAKLLDADAA
                     TVVRNEAVTQMHAMTLDYASPEQVSGGTVTTVSDVYSLGVVLYRLLTGHSPYGVRVND
                     AQRLKEILSDTVPTRPSQVQASDRQRAREIDADLDNILLMALRKEPQRRYGSVEQFAN
                     DLRSYLAGMPVQARGNSLRYRAGKFVRRRKVEIAAGVFVACALFGALLFSIREGRIAE
                     HERHVAQQHFDSVRNLANTMLFQLHDEMAKDAGSLKSREMLVKTSLEYLDALYKQGGS
                     DLELQEELANAYIKVALIQGSDTEANRGNTAGALDSYARAIALLTPLMAADPANFHAG
                     WALAHAHVQQASLLMVVRGPKFARDAAKTGVDLTEKFAPQIADETQRLDRLVTAYVVQ
                     ARILGFMGLPLEAMDSLDKLIGVSEAYWQDHPDDEQALRALGTAYNNAAHIDDPRLKG
                     TAAIDDRAFGLLRKRLSVDEKLLALKPDEFEYQDRLSGTRYNIALRLAARGRFAEAIV
                     RYEQAMPAALKAVARDPDDVQAQYTLALYQTRYAHALFKTGSLEKARVLFVQCDRILE
                     DVFKQDNSLRTQYALGVNAVRLGELYSRLAENSRTGRGAQLDLWRQAQASLKRGNESL
                     ANVTANAKLPSADMVDVVDGVAILARVDATLARLEQP"
BASE COUNT          572 a         1065 c         1008 g          603 t
ORIGIN      
        1 ctcgtcggca accgacaggc agacattcgc acgtcggtcg agaacgaatg gctgtgcgcg
       61 cgcatcgtcg cggcattcgg gttgcccgtc gcgcgctgcg agatcgtcca ggtcgacgat
      121 cagaaggtgc tcatcgtcga acgtttcgac cgcgtgctgc actcgagcgg caagtactgg
      181 ctgcgtctca tgcaggagga tttctgccag gccaccggca caccgtggca tcgcaaatac
      241 caggccgacg gcggccccgg cctcgccgag atggcgcgca tcctgcgtgg ctccgtcgaa
      301 tcgcaggaag acctgcgcac ggtattccgc acccaactac tgttctggat gctggccgcg
      361 accgacggcc acgccaagaa ctacagcatc cgcatcctgc agggcggccg cttccagctc
      421 acgccgctgt acgacgtgct ctccgcgtgg cccatcatcg gcaaacgcgc cagcgagatt
      481 ccgctcgaaa aagtgaagat ggccatggcg atgccgggac agcggcctca ctacctgcac
      541 aagagcatcc agcggcggca tttcgaaacg ctggcgaaac gtctcggtgt cgaggccgat
      601 gcggagaaac tgatcgcgga aatgacgcag aaggcaccgg cgatcatcga ggaggttcag
      661 cgcggattgc cacggaattt tccgcaaggc ctcctcgacc gcgtgctcgg tggcctggcg
      721 aaatccgcca aggcgatggc ctaaggctgc tcgagcctcg caagcgtcgc gtcgacgcgg
      781 gccagtatcg ccacgccatc gacaacgtcg accatgtctg ccgagggcag cttggcattg
      841 gcggtcacgt tcgccaacga ctcgttgccg cgcttcagcg acgcctgcgc ctggcgccac
      901 aaatcgagct gcgcgccgcg gcccgtgcgc gaattctccg caagacgcga atagagttcg
      961 cccaggcgaa ccgcattcac ccccagcgca tattgcgtgc gcagcgagtt gtcctgcttg
     1021 aaaacatcct cgaggatcct gtcgcactgg acgaagagca cccgcgcctt ttcgagactt
     1081 ccggtcttga acaacgcgtg tgcgtatcgc gtttgataga gcgcaagcgt gtattgcgcc
     1141 tggacatcat cgggatcacg cgcaacggct ttcaacgccg cgggcatcgc ctgctcgtag
     1201 cgcacgatcg cttcggcgaa cctgccgcgc gcggccaggc gaagcgcgat gttgtatcgc
     1261 gtccccgaca ggcgatcctg gtactcgaat tcatcgggct tcagcgccag caatttttca
     1321 tcgaccgaca gccgctttcg taacaagccg aatgccctgt catcgatggc tgctgttccc
     1381 ttcagccgcg gatcgtcgat atgcgcggca ttgttgtagg ccgtgcccaa ggcccgcaag
     1441 gcttgctcgt catccgggtg gtcttgccaa tacgcttcgc ttacgccgat gagcttgtcg
     1501 agcgaatcca tggcttcgag cggcagcccc atgaatccca gtatgcgagc ctgcactaca
     1561 taggccgtga ccagtcggtc gaggcgctgc gtttcgtcgg caatttgcgg cgcaaacttc
     1621 tcggtgagat cgacaccggt tttcgccgca tcgcgcgcaa acttcggtcc gcgaaccacc
     1681 atcagcagcg aggcctgctg cacatgcgcg tgggcgagcg cccagcccgc gtgaaagttc
     1741 gccgggtcgg ccgccatcag cggagtcagc agcgcaatgg cgcgcgcata actatccagc
     1801 gctccggcgg tgttgccgcg gttggcttcg gtgtcgctgc cctggatcaa cgcgaccttg
     1861 atgtacgcat tggcgagttc ttcctgcagc tccaggtccg acccgccctg cttgtacagc
     1921 gcatcgagat actcgaggga agtcttcacc agcatctcgc gggacttgag tgagcccgcg
     1981 tctttcgcca tttcatcgtg gagctggaac agcatcgtat tagcgaggtt gcgcacactg
     2041 tcgaagtgct gctgcgcgac gtggcgctcg tgctcggcga tgcggccctc gcgaatcgaa
     2101 aacagcagcg caccgaacaa cgcgcaggcg acgaaaacac ccgcggcgat ttccaccttg
     2161 cgccgtcgca cgaacttgcc cgcgcgataa cgcagcgagt tgccgcgcgc ctgcacgggc
     2221 atgccggcca ggtagctgcg caggtcgttg gcgaattgtt cgacgctgcc ataccggcgc
     2281 tgcggttcct tgcgcagcgc catgagcagg atattgtcga ggtcggcgtc gatttcgcgc
     2341 gcacgttgac gatcgctggc ctgcacctgg ctcggccgcg tcggcacggt gtcgctgaga
     2401 atttccttga gccgctgcgc gtcattcacg cggacgccat aggggctgtg ccccgtcagc
     2461 aaccggtaca acacgacgcc gagcgaatac acgtcgctca ccgtcgtcac ggtgccgcca
     2521 ctcacctgct cgggactcgc atagtccagc gtcatggcgt gcatctgcgt cacggcctcg
     2581 ttccgcacga cggtcgcggc gtcggcatcc agcagcttgg cgatgccgaa atcgagcagc
     2641 ttcaccgacc cgtcggcggt caccaggatg ttgttgggct tgagatcgcg gtggatcacc
     2701 aggtgctggt gcgcgtaact caccgccgcg cagacttgca gaaagagctg cacacgctcg
     2761 cggatcttga gcgagtgcgc gtccgcgaac gtgtcgatgg attcgccgtc caccagctcc
     2821 atgaccacgt acggtgagcc gcccgcggtc gtgccaccgt cgatgagccg cgcgatgttg
     2881 cggtggtcga gaccggccag gatctggcgc tcggtcctga atcgctcgtc ggcgagtttg
     2941 ccgctgacgt ccgcgcgcat gagcttgatc gcgacttcag cgtgatattg gtcgtcgtcg
     3001 cgtaccgcct tgaacacctg gcccatgccg ccggtgccga gaatgccgac gatgcgatac
     3061 gcgccgatgc gttcgccgag gcggctcgtc tcgtcgaccg cgaaggcctg cgctttgagc
     3121 tgagccggta cggcatccag aagtccgctt ggggcatcgt gtgatgtcag cagcgacgcc
     3181 acttcggcga tcagctccga atctcctctc gcctcccgct tgagcaacgc ggggcgttca
     3241 gactctgg
//
LOCUS       DATDEU010000583        10122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_698438, whole genome shotgun sequence.
ACCESSION   DATDEU010000583 DATDEU010000000
VERSION     DATDEU010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698438"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1002..2012)
                     /gene="pdxA"
                     /locus_tag="VIV63_13955"
     CDS             complement(1002..2012)
                     /gene="pdxA"
                     /locus_tag="VIV63_13955"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY6125751.1"
                     /translation="MKLPVIAITMGDAAGIGPEIVMKALATPLVHALCNPLVIGDAAC
                     LRRAGKLVASTLEVDSLADPRDANYSRGRVACIDLPLIPEDLPFGKTSAIAGEAAYRF
                     IERAVRIVEKGGAQAICTAPLSKEALHAAGHRYPGHTELIAALTNTPEVSMMLVAPKL
                     RVIHVTTHIGLLDAIERIDAALVERVIGRAQETLVRAGIASPRVGVCGINPHAGENGL
                     FGRGEEASKIVPAVEACRARGWNVTGPLPADTLFFLAARGDYDIVVAMYHDQGHGPIK
                     VLGLESGVNITVGLPVVRTSVDHGTAFDIAGKGVADERSLIEALRQAAELAAAGASRG
                     AG"
     gene            complement(2009..3283)
                     /locus_tag="VIV63_13960"
     CDS             complement(2009..3283)
                     /locus_tag="VIV63_13960"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEY6125752.1"
                     /translation="MEPAWFILSDDLTGAADTAIAFARRRLPATVVWGDAHPEDETRS
                     IALAYDAATRELGAAEAARRHRDVLRRFLRPRMFVFKKIDSTLRGHPAEEIAAMLDVM
                     LAHHEGVRVVLAPSFPAMGRTVRDGRVQVHGIPLPFTEYWPEGREPDLASLVNLLESA
                     GVHARQVSLSCVRADPSALRAEFARSASHAEHHAVAVCDAETADDLARIASASLEDGV
                     TFFIGSAGLAHAIAQCVARDSARRLTSPIRQGESRGALVVVGSQASASRAALAPLLAL
                     ENVEGVTLRPAMLMGDLQSAAHAEIAQAIGVALSRGTDVVVDIGQDVADPDRGNSQFV
                     MALARVLAPAARQASALVVTGGETAAAVLTRIGVAGIRLVDEIEPGIPLGLTLGEVSV
                     PAVTKAGGFGNEECLKRIVSRLRFIRQTGTVA"
     gene            complement(3288..4247)
                     /locus_tag="VIV63_13965"
     CDS             complement(3288..4247)
                     /locus_tag="VIV63_13965"
                     /inference="COORDINATES: protein motif:HMM:NF015751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HEY6125753.1"
                     /translation="MKQVPIKRAIERVPGGLMVVPLLTGSLLATFLPGAPQFFGSFTG
                     ALFTGALPILAVFFVCTGASIDFRATPYILKKGGTLFGVKVGIGILLGLLFGRLLGEA
                     PISAGVFAGISTLAIVAAMNDTNGGLYMALMGQYGHPRDVGAYSVMSLESGPFLTMVT
                     LGVAGLSAFPWPTLLGGILPLLAGMALGNLDREMREFLSRAIPVLIPFFAFALGANLD
                     LGKVWLAGLLGLGLGVAVVVVTGIPLFFADRLTGGSGVAGVAAASTAGNAVAVPAIIA
                     SANPAYQEAAAHATILVAASVVVTAILVPLVTAWTARRVGERA"
     gene            4287..4733
                     /locus_tag="VIV63_13970"
     CDS             4287..4733
                     /locus_tag="VIV63_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125754.1"
                     /translation="MNKYTRIALVGSLLAASFVSHAEDAKKAKVDCTMKFSLSGWSAF
                     YKRADGTGTVTCNNGQTANVKLEARGGGLTAGKSSIEDGKGEFSNVKNIEEIFGKYAN
                     AEASAGAVKSSGAQAMTKGEVSLALAGTGRGWDLGISFGKFTIKRL"
     gene            complement(4720..5601)
                     /locus_tag="VIV63_13975"
     CDS             complement(4720..5601)
                     /locus_tag="VIV63_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY6125755.1"
                     /translation="MGAVKRKRTFWQVLKGTTGNWLDDQASSISAALAFYCAFSLAPL
                     LIIIVSIAGWIVGGELAYSYVGSQLTMLFGQQSATLILEAMRSAQTTEGTWATIVSVV
                     MLLVGASTVFAALESALQQVWGGRDSLPRGWRSFVRSRLISFGFILAIGFLLLVSLTL
                     TTALAALRGYVLRHFEGLVGILASLDFVLSIVLGTGLVALMYRYLPARRLAWRHVITG
                     ALVTALLFHLGRWAIGLYLGRATQPTAYGAAASFAALLLWLYYSAQIFLFGAEFTACL
                     GQSRTPKPLAPGATTSA"
     gene            complement(5681..6715)
                     /locus_tag="VIV63_13980"
     CDS             complement(5681..6715)
                     /locus_tag="VIV63_13980"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY6125756.1"
                     /translation="MLIAGRLLRIIVAAAGLALAACAARPPVTGEVLISGQTMGSAWT
                     AKVVGAAPGSAAELQAGAQARFDAVNLALSTYRADSALSQFNADESGEWVSVDPELAT
                     VLAYALSLAERSGGAYDVTVGPLVNLWGFGPEPATRQVPGSAAIDAARARVGWRGVEV
                     DLAALRARKRPGMRVDLSSLGKGRGVDRVAEYLDSRGVTNYLIDLSGKLRARGRNARG
                     EPWRVAVERPAPDVSSDVPAIEPAIVALQDQSVATAGDYRRYFESGGRHYSHIIDPRT
                     GYPVTHGTVSATAIAADCMQADALATVLMVLPPAEALKLAEQLRLRALLIELRDGKYP
                     VLRSTTWQAH"
     gene            complement(6717..7523)
                     /locus_tag="VIV63_13985"
     CDS             complement(6717..7523)
                     /locus_tag="VIV63_13985"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEY6125757.1"
                     /translation="MKKSFVTMAAMLVVLAPATSEAARAWLLPSQTILARSGGWVTVD
                     AAMADDLFSFNQGAMPIDSLIVSGPDGQPVAIQNPAKGKTRSSFDLELRQSGTYRIAV
                     ADNMVMARWEEDGKPKRWRGTPAEMATGVPANAAKLEVEQTQRRVETFVSVGTPTDVK
                     AAAGSGIELTPVQHPNDLYARETAKFRFLLDGKPAADLEVEVIAGGTRYRDSPDEMIF
                     KTGKDGEIAITWPAAGMYWLEVSLQDEKATVPGVKKRRASYSGTFEVLTQ"
     gene            complement(7534..8046)
                     /locus_tag="VIV63_13990"
     CDS             complement(7534..8046)
                     /locus_tag="VIV63_13990"
                     /inference="COORDINATES: protein motif:HMM:NF021540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HEY6125758.1"
                     /translation="MIRKTLFALTLALGAVGGAAHAADLSLSVEIPQLPVAEYHRPYV
                     AIWIEGADQNIAANLAVWYQVRGDHTKWLPDLRQWWRRGGRDLKVPVDGLTGATRPVG
                     QHLLKFDAAQAPLAGLKPGQYTMVVEAVREVGGREAVRIPFEWPIKAAKRGSANGSKE
                     LGAVALTLNP"
     gene            complement(8073..8678)
                     /locus_tag="VIV63_13995"
     CDS             complement(8073..8678)
                     /locus_tag="VIV63_13995"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY6125759.1"
                     /translation="MDPRRRMQLYRTMHLWHWISAAVCFAALTLFTVTGITLNHASAI
                     SAEPEVTSGEARLPENLRATLATANTDVVPADIAAWAEDSFDVSLRDAAAEWSEEELY
                     LSAPGPGRDAWVSIDRASGTAKYESTDRGWLAYFNDLHKGRNTGIAWIVFIDVVAVAV
                     LFFSLTGLVLLWIQARQRKSTWPLVGGGIALVTVLMIFLVH"
     gene            complement(8769..9788)
                     /locus_tag="VIV63_14000"
     CDS             complement(8769..9788)
                     /locus_tag="VIV63_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125760.1"
                     /translation="MKCIMSMALVCMFTAGAATAQGPVALLPSPLVMPGRIGPMIQVG
                     TPHKYDDPRLGMSYQYSGEGLSLTVYVYDTGNSELPDGADTIPSCQEFEIAKQGVNQA
                     YQKATLKSEYLVRLNPPDEFPQMREALYEYEREGQPTISFIWVTTVAKHFVKLRMSLN
                     AKLRDEVPDARKAVLSTVGEAIKPHLKPVDPKAEPPGTSLNVNALGGSDDDMASGLMY
                     LVLLSAVAEQAPELVPVCGGEFVPGYDAELDLYRGMIAMNGEDDATRSGKQLAKVEKA
                     GFLEEFVWVELHRESWGETAPSGLTLPEYQAWRKKNLKRFKPPSFGSVVVQHPRALPP
                     EPVAP"
     gene            9935..>10122
                     /locus_tag="VIV63_14005"
     CDS             9935..>10122
                     /locus_tag="VIV63_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125761.1"
                     /translation="MRANKILLTVTLATAGVGVHADVTINTTTSGKASIMDVSGIGVH
                     QIKGARQRSDQTIGGKPQV"
BASE COUNT         1894 a         3326 c         3220 g         1682 t
ORIGIN      
        1 acggccgcgc gtcctctgcg ggcgtggccc gcaaaaagtt tgctgttcat gatttcccct
       61 tcgttttaag acttcagacc ggaacggggg tcgagttttc cgcgctctgc cccccgctgc
      121 cgcccgatca tgtatgagcg aatttgcttg agcaagaccg aaaaccgatc tttctcgcgc
      181 gaggatgacc ctcctgaagg ctaatttcat tcagggatgg gcatttacga gcgtgcgatc
      241 acccgtttga gcggaaggat atgagagcgg agataggtgg agcgccgcca gcgaaggggt
      301 gctagcggcg ctccgattgt cgaaaagcac acagagcgcg gacaaggtgc acttctcgga
      361 ctcgacgaag cttccgtggg aagcttcaat aaaccgtgga tccgtatcag ttgttagcgg
      421 tcacaaacgc tcaatactgt tcaggatcaa tcaaatcggt gcttttttta tcacaactcc
      481 ccgaaaagct cgataaaaat cgtatttatc atccgattaa gccggggttt ggaggctaca
      541 accctggtaa cgctacccgc tagaaaatct ttgtcaccgg tggaaatgga tgcgccggca
      601 ggcagcttcc aacgcaaggc ggtaaagaaa tcggcggcgg cgtgtggcca ggctttggga
      661 gacaggggct ccgccacacc aggaatgaat tccaagaacg ttgtcagtgg cgcaagccct
      721 cgggcgagac gctgctaccc gacgaaagcc gttttctcgg cggcggcgac gacagcggag
      781 cgtacgtgca cgccgtggac tggccttaca cctccccggg cgcgccgcag tactggcccc
      841 gttctctgaa gaccatcgtt cgcaggttgc gcgactcacg ttgtgcgatg gggaagttcc
      901 ggggcgtgga acgcaggtag ttctgcaacg atgcctatct acccaagcgc gaccgggtca
      961 tgggcgcgcg atcggagaag gtctgggccg agatctggcg cctaaccggc gccgcgggaa
     1021 gcgcccgctg ccgcgagttc cgctgcctgg cgcagcgcct cgatgaggct gcgttcgtcg
     1081 gcgacgcctt tgcccgcgat gtcgaatgcc gttccatggt ccaccgacgt tctcaccacc
     1141 ggcaggccga cggtgatatt cacgcccgac tcgagaccca ggaccttgat gggaccgtgg
     1201 ccctggtcgt gatacatggc gacgacgatg tcgtagtcgc cgcgcgcggc caggaagaac
     1261 agggtgtcgg ccggcaacgg accggtgacg ttccagccac gggcgcggca ggcctcgact
     1321 gcgggaacga tcttcgacgc ttcttcaccg cggccaaaca agccattttc accggcgtgc
     1381 gggttgatgc cgcagacacc tacacgggga ctcgcgatgc cggcgcgcac gagcgtctcc
     1441 tgcgcgcggc cgatgacacg ctccaccagc gcggcatcga tgcgctcgat ggcgtccagc
     1501 aaaccgatgt gcgtcgtcac gtgaatgacg cgcagcttcg gcgcgaccag catcatggaa
     1561 acttccggcg tgttcgtgag cgccgcaatc agctcggtgt gaccggggta ccgatgaccc
     1621 gcggcgtgca aggcttcctt gctgagcggc gccgtgcaga tggcctgtgc cccgcccttc
     1681 tcgacgatac gcacggcgcg ctcgatgaac cggtaggccg cctcgccggc gatcgcggac
     1741 gtcttcccga aaggcagatc ctcgggaatc aacgggaggt cgatgcaggc aaccctgccg
     1801 cgtgaatagt tcgcgtcgcg cggatccgcc aggctatcta cctcgagcgt actcgccacc
     1861 agttttccgg cccggcgcaa acacgcagcg tcgccgatca ccaggggatt gcagagcgcg
     1921 tgtaccagcg gtgtggccag cgccttcatg actatttcgg ggccgatgcc ggcggcatca
     1981 cccatggtga tcgcgatgac aggcagcttc atgccaccgt tcccgtctga cggatgaacc
     2041 gaagtctcga gacgatgcgt ttcaaacatt cctcattgcc aaacccgccg gccttggtca
     2101 ccgcgggcac cgacacttcc ccgagtgtaa ggccgagcgg gatgccaggc tcgatctcgt
     2161 ccacaagtcg aatgccggca acgccgatgc gcgtgagcac ggctgccgcg gtttcaccgc
     2221 cggtgacgac cagtgcgctg gcctgccggg ccgccggcgc cagcacacgt gccagcgcca
     2281 tgacgaactg cgagttgcca cggtccgggt ccgcgacatc ctgaccgatg tcgacgacca
     2341 catcggttcc tctcgagagc gcgacgccaa tcgcctgcgc gatttcggca tgagccgcgg
     2401 actgcagatc tcccatcagc atcgcgggtc ggagggtgac gccttccacg ttttcgagcg
     2461 ccagcagggg cgcgagagcg gcgcgtgatg ccgatgcctg cgaacccacg acgacgagcg
     2521 cgccccggga ctcgccctgg cgaatcggcg acgtgagtcg tcgagcgctg tcgcgcgcca
     2581 cacattgcgc gatcgcgtgt gcgagccctg cgctgccgat gaagaaggtg acgccgtctt
     2641 cgagactcgc cgaagcgatt ctcgcgaggt catccgccgt ctcggcatcg cagacggcga
     2701 ccgcatggtg ttccgcatgg ctcgcactgc gggcgaactc ggcgcgcagc gccgaagggt
     2761 cggcacgaac gcacgacagt gaaacctggc gcgcgtgcac gcccgcgctt tcgagcaggt
     2821 tcacgagact ggcgagatcg ggctcgcgac cctcgggcca atattccgtg aatggcagcg
     2881 ggatgccgtg cacctgtacg cgcccgtcgc gtacggtgcg gcccatcgcg ggaaacgagg
     2941 gcgccaggac cacgcggaca ccctcgtggt gcgccagcat cacatccagc atcgcggcaa
     3001 tttcctcggc cggatgtccg cgcagcgtcg agtctatctt cttgaagacg aacatccgcg
     3061 ggcgcaggaa gcggcgcagc acatcacggt gacggcgcgc ggcttcggcg gcaccgagct
     3121 cacgcgtggc ggcgtcgtaa gccagcgcga tcgaccgggt ctcgtcttcg ggatgtgcat
     3181 cgccccacac caccgtcgcg ggcagccgtc gccgcgcaaa cgcgatggcg gtatccgcgg
     3241 cacccgtgag gtcatcggag aggatgaacc acgccggctc catctattca ggctcgctcg
     3301 ccgacgcgcc gcgccgtcca ggccgtcacc agcggcacca aaatcgcggt cacgacgaca
     3361 ctcgcggcga ccaggatcgt cgcgtgggcc gctgcttcct ggtaggccgg attggcgctc
     3421 gcgatgatcg cgggcactgc caccgcgttg ccggccgtgg atgcagccgc cacgcccgcg
     3481 acgccactgc caccggtcaa ccgatcggcg aagaacagcg ggatgccggt gaccacgact
     3541 accgccaccc cgagccccag ccctaacaac cctgccagcc agactttgcc gagatcgaga
     3601 ttggcgccga gggcgaatgc aaaaaacgga atgagcacgg gaatggcccg gctcaggaat
     3661 tcacgcatct cgcgatcgag attcccgagc gccatgcccg ccagcaacgg caggatgccc
     3721 cccagcaacg tcggccacgg aaacgctgac aatcccgcaa cgccgagcgt gaccatggtc
     3781 aggaaaggac cggactcgag cgacatgacg ctgtaagcgc cgacatcgcg tggatggccg
     3841 tactggccca tcagcgccat gtacaagccg ccgttggtgt cgttcatcgc agccacgatg
     3901 gcgagcgtgg agattcccgc gaacacgccc gcactgatgg gcgcctcgcc cagcagccgg
     3961 ccgaacagca ggcctaacaa gataccgatg ccgactttga cgccgaacag agtgccaccc
     4021 ttcttcaaaa tgtagggcgt ggcgcggaag tcgatgctcg cgccggtaca cacgaagaac
     4081 accgcgagga tgggcaacgc accggtgaac agcgcgccgg tgaaggatcc gaagaattgc
     4141 ggcgcacccg gaaggaaggt ggccagtaat gatccagtta acaatggcac gaccatgagg
     4201 ccgccgggca cgcgctcgat ggcccgcttg attggaacct gcttcattct tgccatcccc
     4261 tgccggcacg ggtagcgtgc cgccccatga acaaatatac ccggatcgca ctcgtcggca
     4321 gcctgttggc ggccagcttc gtctctcacg ccgaggacgc caagaaggcc aaagtcgatt
     4381 gcacgatgaa gttttccttg agtggctggt cagcgttcta caagcgtgcc gacggcacgg
     4441 gcaccgtcac ctgcaacaac ggtcagacgg ccaacgtgaa actcgaggca cgcggcggag
     4501 gcctgaccgc gggcaagtcc agcatcgagg acggcaaagg cgagttctcg aacgtgaaga
     4561 acatcgaaga gatcttcggc aaatacgcca atgccgaagc cagcgcgggc gctgtgaaat
     4621 cgtctggcgc tcaggcgatg accaagggcg aagtctcgct ggcattggct ggcaccggcc
     4681 gcggctggga cctcggcatc tcgttcggca agttcaccat caagcgcttg tagtagcgcc
     4741 cggagccagc ggcttcggcg tgcgcgactg acccagacaa gccgtgaact cggcaccgaa
     4801 cagaaagatc tgcgccgagt agtagagcca cagcagcagc gccgcgaacg aagccgcggc
     4861 gccataggca gtcggctggg tcgcacgtcc caggtacagg ccgatggccc aacgtcccag
     4921 gtggaacagc agcgcggtca ccaacgcacc ggtgatcacg tgccgccagg ccaggcgccg
     4981 cgccggtaga tagcggtaca tgagcgcgac caggccggtg ccgagcacga tcgacaggac
     5041 gaagtcgagt gacgccagga ttcccaccag cccctcgaag tggcgcagca cgtagccacg
     5101 caaggcggcc agtgccgtgg tcagcgtcag cgagaccagc agcaggaagc cgatcgcgag
     5161 gatgaatcca aatgagatga gtcgcgagcg tacgaaggag cgccagccgc gcggcagcga
     5221 atcgcggcct ccccacacct gttgaagcgc gctctcgagc gcggcgaaca ccgtgctcgc
     5281 gccgaccagc agcatgacca cgctgacgat cgtcgcccag gttccctcgg tggtttgcgc
     5341 gctgcgcatc gcctcgagga tcaacgtcgc gctttgctga ccgaacagca tggtgagctg
     5401 gctgccgaca taggaatacg caagctcgcc gccgacgatc cagccggcga tcgacacgat
     5461 gatgatgagc agcggcgcga gggaaaacgc gcaatagaac gccagcgcgg cgctgatgct
     5521 cgacgcctgg tcatcgagcc agttgccggt cgtgcctttg agaacctgcc aaaaagtgcg
     5581 cttgcgcttc acagcaccca tgttgccgca gctcccggtc taacaacccg gcaatcctgt
     5641 cagcgcgggt tggcgccagt cgccagagca cgcctacagc tcagtgtgct tgccaggtgg
     5701 tgctgcgcag caccgggtat ttcccatcgc ggagttcaat gagcaatgca cgaagacgca
     5761 attgctccgc cagtttgagc gcctcggccg gcggcaacac catcaacacc gtcgcgagtg
     5821 cgtcggcctg catgcagtcg gcggcgattg ccgtggccga caccgtgccg tgtgtcacgg
     5881 ggtatccggt tctcggatcg atgatgtgcg aatagtgccg cccgccgctc tcgaagtagc
     5941 ggcgatagtc gccggcggtg gcgacgctct gatcctgcag tgccacgatc gcaggttcga
     6001 tcgcgggaac gtcggacgac acgtcgggcg ccgggcgctc caccgcgacg cgccacggct
     6061 cgccacgcgc attgcgaccg cgcgcgcgca gcttgcccga caggtcgatg aggtagttag
     6121 taacgccgcg ggaatccaga tactccgcga cccggtcgac gccgcgtccc ttgccgagcg
     6181 acgacaggtc gacgcgcatg ccagggcgct tgcgggcacg aagcgccgcg aggtcgacct
     6241 cgacgcctct ccagcccacg cgggcgcgcg cggcatcgat cgcggcgctg cccggcacct
     6301 gacgcgtcgc aggctcgggt ccaaagcccc acaggtttac gagcggtccg acggtgacgt
     6361 cgtacgcgcc gccactgcgc tccgccaggc tcaacgcata cgccagcacc gtggcgagtt
     6421 ccggatcgac gctcacccac tcgccgcttt cgtcggcgtt gaactggctg agtgcggaat
     6481 cggcgcgata cgtgctgagc gcaaggttca cggcatcgaa ccgcgcctgc gcacccgcct
     6541 gcagctccgc ggcggaaccc ggcgccgcgc ctaccacctt ggcagtccag gcgctgccca
     6601 tcgtctgacc cgaaatcagg acctcgccgg tgacgggcgg tcgcgccgcg caggcggcga
     6661 gcgcgaggcc cgccgccgcg acgatgatgc gcagcaggcg accggcgatc agcaaactac
     6721 tgcgtcagca cttcgaacgt gcccgagtag gaggcgcggc gtttcttcac acccggcacc
     6781 gtcgccttct cgtcctgcag cgacacttcg agccagtaca taccggctgc aggccaggtg
     6841 atcgcaatct cgccatcctt gccggtcttg aagatcattt cgtccggcga atcgcggtaa
     6901 cgcgttccac cggcgatgac ttccacttcg agatccgcgg ccggcttgcc gtcgagcagg
     6961 aaacggaact tcgcggtttc gcgcgcgtac aggtcattcg ggtgttgaac cggagtcagc
     7021 tcgatgcccg atcccgcggc cgccttcacg tcggtgggcg tgcccacgct cacgaaagtc
     7081 tccacgcggc gctgcgtctg ctcgacttcg agcttggccg cgtttgccgg aactcccgtg
     7141 gccatttccg cgggcgtacc gcgccagcgt ttcggcttgc cgtcttcttc ccagcgcgcc
     7201 atcaccatgt tgtccgcgac cgcgatgcgg tacgtgccgg actgccgcag ctcgagatcg
     7261 aagctgctgc gcgtcttgcc cttcgccggg ttctgaatcg cgaccggttg tccgtcggga
     7321 cccgagacga tcagcgagtc gatgggcatc gcgccctggt tgaacgagaa aagatcgtcg
     7381 gccatggcgg cgtccactgt cacccagccg ccggagcgcg cgaggatggt ttgcgaaggc
     7441 agcaaccagg ctcgcgctgc ctcgctggtc gcgggcgcca gcacgacgag catcgctgcc
     7501 attgtcacga atgatttttt catgtgattc ccctcaagga ttcagcgtca gcgcgaccgc
     7561 gccgagctct ttgctgccgt tcgcagaacc gcgtttggcg gctttgatag gccactcgaa
     7621 cgggatgcgc accgcctcgc gtccacccac ttcgcgcacc gcttccacga ccatcgtgta
     7681 ctggccaggc ttcagtcccg cgagcggcgc ctgcgccgcg tcgaacttca gcaggtgctg
     7741 gcctaccggc cgggtcgcac ccgtcaatcc atctaccggg actttcaggt cgcggccgcc
     7801 acggcgccac cactggcgca gatccggcag ccacttcgtg tgatcgccgc gcacctggta
     7861 ccagaccgcg aggttggcgg cgatgttctg atcggcgcct tcgatccaga tggcgacgta
     7921 cggacgatgg tattcggcca ccggcaactg cggaatttcc acgctgaggc tcaaatcggc
     7981 cgcgtgggcc gcgccaccga cggcgccaag cgccagtgtc agcgcgaaca gggtcttgcg
     8041 aatcattcgg tgctccgtct gggaaagact aactagtgga cgaggaagat catgaggacg
     8101 gtgacgaggg cgatgccgcc gccgaccaat ggccacgtgg acttgcgctg ccgcgcctgg
     8161 atccacaaca gcacgaggcc ggtcagcgag aagaacagca ccgccaccgc gacgacatcg
     8221 atgaagacga tccaggcaat gcctgtatta cggcccttgt ggagatcgtt gaagtacgcg
     8281 agccagccgc gatcggtcga ttcgtatttc gctgtaccgc tggcgcggtc gatcgagacc
     8341 caggcgtcgc gacccggacc cggagccgac aggtacagct cttcttcaga ccactccgcc
     8401 gcggcgtcgc gcagcgacac atcaaatgag tcctccgccc aggcggcaat gtccgccgga
     8461 acgacgtcgg tattcgccgt ggccagagtg gcgcgaagat tttccggtaa gcgggcctcg
     8521 ccgctcgtca cttccggctc ggcgctgatc gcgctggcgt ggttgagcgt gatgccggtg
     8581 acggtgaaca gcgtgagtgc cgcgaaacac accgccgcgc tgatccaatg ccacaggtgc
     8641 atcgtgcggt acagctgcat gcggcggcgg ggatccatcg gcgttcggct ccaaaaagag
     8701 cgcgcacgct acatgagaac tgttcttatt tgcaaccgaa ctcttctata aggggcccga
     8761 cgcggctttc agggcgcaac cggctccggc gggagtgcac gcgggtgctg gacgaccacg
     8821 ctgccgaaac tcggcggctt gaaacgcttc aggttcttct tccgccaggc ctggtactcg
     8881 ggaagcgtca acccggatgg cgcggtctcg ccccaggact cccgatgcag ctccacccag
     8941 acgaattctt ccagaaagcc ggccttctcc actttcgcca gttgtttgcc ggaccgggtg
     9001 gcgtcgtcct cgccgttcat cgcgatcatg ccgcgataca gatcgagttc cgcgtcgtaa
     9061 cccggaacga attccccgcc gcagacggga acgagttcgg gtgcctgttc ggcgacggcg
     9121 gagagcagca ccaggtacat gagccccgaa gccatgtcgt catcggagcc ccccagcgca
     9181 ttcacattca gcgaagttcc gggaggctcc gccttgggat ccacgggttt cagatggggc
     9241 ttgatcgcct cgccgacggt cgagagcacg gcctttctcg cgtcgggcac ttcgtcgcgc
     9301 agtttcgcgt tcaggctcat gcgcagtttc acgaagtgct tcgcgaccgt cgtgacccag
     9361 atgaaggaga tggtcggctg accttcgcgc tcgtattcgt agagcgcctc gcgcatctgc
     9421 ggaaactcat cgggcgggtt gagccggacc aggtattccg atttcagcgt cgctttctgg
     9481 taagcctggt tcacgccctg cttcgcgatt tcgaattcct ggcaggaggg tatggtgtcc
     9541 gcgccgtcgg gcagttcgct attgccggtg tcgtagacat agaccgtgag cgacaggccc
     9601 tcgccgctgt attggtacga catgccgagt cgcggatcgt cgtacttgtg aggggtgccc
     9661 acctggatca tcggcccgat gcgccccggc atgacgaggg gtgagggaag cagcgcgacg
     9721 ggcccctggg ccgtcgcggc gcccgcggtg aacatgcaga ccagcgccat cgacataatg
     9781 catttcatgc gaactcccgc cggcaacccg ccagtagtgt agcgaaaccc gcattgcttt
     9841 cggtctaact gtcggctggt cgagacattc tcgaccgccc tgcgaatcgg gtatacaggc
     9901 gtccgatcaa ctaactaaat ggggctggct gtccatgcgc gctaacaaga tactgctgac
     9961 tgtcacgctg gccaccgccg gagtcggcgt ccacgccgac gtcaccatca acaccactac
    10021 gagcggcaag gcatcgatca tggatgtgag cggtattggc gtgcaccaga tcaagggcgc
    10081 gcgccagcgc agtgatcaga cgatcggcgg caagccgcag gt
//
LOCUS       DATDEU010000584         3517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_554216, whole genome shotgun sequence.
ACCESSION   DATDEU010000584 DATDEU010000000
VERSION     DATDEU010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554216"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VIV63_14010"
     CDS             <1..300
                     /locus_tag="VIV63_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY6125762.1"
                     /translation="SEKPGMTTIYVGNLPFSATEQDIKVLFERHGKVDSVKLINDRET
                     GKPRGFGFVEMGSGEAQAAIQGLNGYQMNGRPLRVNEAQERPPRPRQGGGGPFRR"
     gene            complement(468..2555)
                     /locus_tag="VIV63_14015"
     CDS             complement(468..2555)
                     /locus_tag="VIV63_14015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY6125763.1"
                     /translation="MSTAEQLGSLNAPQRKAVTYGEPLPGGKGFRAGPLLIVAGAGTG
                     KTNTLAHRVAHLALSGVDPARILMLTFTRRAALEMKRRANEIMREALDDKLGGKASAI
                     SQRLTWSGTFHSIGNRLLRHYAKHVGLDPAFSVIDRSDSADMVDTVRTELGYSGKGQR
                     FPRKDTCLAIYSHRVNTQKSLKETLETQYSWCAHWEEDIAKLLRGYVERKSQSRVLDF
                     DDLLLYWHAMMSEDRIAKSVAAHFDHVLVDEYQDTNKLQGDILAALKPDGQGVTVVGD
                     DAQAIYSFRAAAVENILGFPDRFRPKAEVVTLAQNFRSSQSILDSANALMADAPRQHR
                     KHLLSMRGQGPRPKLVTVDDLPTQAEFIATRVLASREAGVPLKRQAVLFRSGSHSDVL
                     EIELAKRKIPFVKYGGLKFLDAAHVKDLLAVLRWADNPHNQLAAFRVLQFMPGMGPAN
                     AKRALDHFVASGYLWNSFEKFEPPTDARVAWRKFCELFVALGDPAREWRGQIHLIREW
                     YKPHMERMYDHVHTRIGDLDQLELLSSQHQTRERFCTELTLDPPYATSDLAGQPVMDE
                     DYLVISTVHSAKGMEWDNVFVLNVVDGSFPNEFANKAELVEEERRVLYVAMTRARNEL
                     TCVVPLRFQITSQPKTSDGHVYGGRSRFLTEKVIKCFDEQTFQGSNTEVKIDETPTES
                     GAIDVASRLKQMW"
     gene            2851..3375
                     /locus_tag="VIV63_14020"
     CDS             2851..3375
                     /locus_tag="VIV63_14020"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6125764.1"
                     /translation="MLFERKEIFEGRVIKVSVDTVDLPNGVRLPLEIVRHPGGAAAVA
                     IDAHNRICLLRQYRHAAGGYIYELPAGKLEPGEPPEVTVRRELVEEGAVSAASWESLG
                     AFFSSPGVFTEVIHLYLATDLTPAESAPEAGEVFEVEWWPLEKAVEKARSGELTDGKT
                     IIGILRAASHRKFA"
BASE COUNT          651 a         1067 c         1138 g          661 t
ORIGIN      
        1 agtgagaagc ctgggatgac gacgatctac gtgggcaatc tgcccttctc cgcaaccgag
       61 caagacatca aggtgctctt cgagcgccat ggcaaggttg attccgtcaa gctgatcaat
      121 gaccgcgaaa cgggcaagcc ccgtggattt ggtttcgttg agatgggatc cggcgaagca
      181 caagccgcga ttcagggtct caacggttat cagatgaatg gccgtccgct gcgcgtcaat
      241 gaagcgcagg aacgtccgcc gcgcccgcgt cagggtggtg gcggtccgtt ccgccgctga
      301 ttcgacgccg gactaacaag cggcgcacag atccatacga accccgccgt gtgcggggtt
      361 catttttctt tgtccgggga atgtgaaggg ggcaaataaa aggggacaga tctattttcc
      421 gcagcggaaa atagatctgt ccccttttat ttgccccctt ttattcttca ccacatctgc
      481 ttgaggcggg acgccacgtc gatggcgccc gactccgtcg gggtctcgtc gatcttcact
      541 tccgtgttcg agccctggaa cgtctgctcg tcgaaacact tgatgacctt ttcagtcagg
      601 aagcggctgc ggccaccgta cacgtgcccg tccgaagtct tcggctggct ggtgatctga
      661 aaacgtagcg gtacgacgca ggtgagttca ttgcgcgcgc gcgtcatggc gacgtacaac
      721 acgcggcgtt cctcttcgac gagttcggcc ttgttggcga actcattggg aaagctgccg
      781 tcgacgacgt tgaggacgaa aacgttgtcc cattccatgc ccttggcgga atggaccgtg
      841 gagatgacga ggtagtcctc gtccatcacc ggctggcccg cgaggtcgct ggtcgcatac
      901 ggtgggtcga gcgtgagctc ggtgcagaaa cgctcgcgcg tctgatgctg cgatgacagc
      961 agctcgagct ggtcgaggtc gccgatgcgc gtatgcacgt ggtcgtacat gcgttccatg
     1021 tgcggcttgt accactcgcg aatgaggtgg atctgaccgc gccattcacg ggcgggatct
     1081 ccgagcgcca cgaagagctc acagaatttc cgccacgcga cgcgcgcgtc cgtcggcggc
     1141 tcgaacttct cgaacgagtt ccacaggtag ccgctggcca cgaagtggtc gagcgcgcgc
     1201 ttggcgttcg ccgggcccat gccgggcatg aactgcagca cgcggaaggc cgcgagctgg
     1261 ttgtggggat tgtcagccca gcggagcacc gccagcaggt ccttgacgtg cgcggcgtcg
     1321 aggaacttga ggccgccgta cttcacgaaa ggaatcttgc gcttcgccag ttcgatttcg
     1381 agcacgtcgc tgtgcgaacc gctgcggaac aggactgcct ggcgcttgag tggaacgccg
     1441 gcttcgcgcg aagcaagcac gcgcgtcgcg atgaactccg cctgcgtggg cagatcgtcg
     1501 accgtcacga gcttcggacg cgggccctgg ccgcgcatcg acagcaggtg cttgcgatgc
     1561 tgcctcggcg catccgccat gagcgcgttc gccgaatcga ggatgctctg gctcgaacgg
     1621 aaattctgcg caagcgtgac gacttcggcc ttggggcgga agcggtccgg gaaaccgagg
     1681 atgttctcga ccgccgccgc gcggaacgag tagatagcct gcgcgtcgtc gccgaccacc
     1741 gtgacgccct gcccgtccgg cttgagcgcg gcgaggatgt cgccctgcag cttgttggtg
     1801 tcctggtatt cgtcgaccag cacgtggtcg aagtgcgcgg cgaccgactt ggcgatacgg
     1861 tcttcgctca tcatcgcgtg ccagtagagc agcaggtcgt cgaaatcgag cacgcgcgac
     1921 tgggacttgc gctccacgta accgcgcagg agcttggcga tgtcctcttc ccagtgcgca
     1981 caccaggagt actgcgtctc gagcgtttcc ttgagcgatt tctgggtgtt gacgcggtgg
     2041 gagtagatcg cgaggcaggt gtccttgcgc gggaagcgct ggcccttgcc ggaataaccg
     2101 agttcggtgc gcaccgtgtc gaccatgtcc gcggagtcgg aacgatcgat gacgctgaaa
     2161 gcaggatcga ggcccacgtg cttggcgtag tgacgcagca ggcggttgcc gatggaatgg
     2221 aacgtgccgg accaggtgag ccgctgcgag atggcggagg ccttgccgcc gagtttgtcg
     2281 tcgagcgcct cgcgcatgat ctcgttggcg cggcgcttca tctcgagcgc cgcacggcgc
     2341 gtgaacgtga gcatgaggat gcgcgccggg tcgacgccgc tcagtgccag atgcgcgacg
     2401 cggtgcgcga gcgtattggt cttgccggtg ccggcgccgg cgacgatcag caacgggccc
     2461 gcgcgaaagc ccttcccgcc gggaagaggc tcaccgtacg tcaccgcctt gcgttgcgga
     2521 gcgttcagac ttccgagttg ttcggccgta gacatcaata caacaatata tgaaaggcgg
     2581 ttgatattag gcccggctcc ccaagagccg tgacgcccgt cacgcccgaa tccatccgga
     2641 atgtgacgcc tttttgatct cgatttgcgc cgcgatttgc acgccggcgg caggtggcgg
     2701 cgattatgag cgcgggccct cggccggtac aagcgtcacc gcgcctgttg cctgcacgca
     2761 tcgagcgctg caaacaactc atccgccatc gtcttactac tgtaggcact gtcgtacgtg
     2821 actcgcatgc cgggcaggta cactcgcgcc atgctgttcg aacgcaaaga aatcttcgaa
     2881 ggacgcgtca tcaaggtgag cgtcgacacc gtggacctgc ccaatggcgt gcgcctgccg
     2941 ctcgagatcg tccggcatcc ggggggcgcg gctgcagtgg ccatcgatgc ccacaaccgc
     3001 atctgcctgc tgcgccagta tcgccacgcc gcgggcggtt acatctacga gttgccggcc
     3061 ggcaaactcg aacccggcga accgcccgaa gtcaccgtgc ggcgtgagct cgtggaggaa
     3121 ggggccgtgt cggcggcgag ctgggaatca ctcggcgcct tcttcagctc gccgggtgtg
     3181 ttcaccgaag tcatccatct ctacctggcg acggacctga cgccggccga atccgctccc
     3241 gaagccggcg aagttttcga agtcgaatgg tggcctctcg agaaggccgt ggagaaggcc
     3301 cgcagtggcg agctcaccga cggcaaaaca atcatcggca ttttgcgggc cgcgtcacac
     3361 cgaaaatttg cgtgaaacgc ttcacaagcc gcgattcgaa gctgccggaa tggtggctat
     3421 gtcaaacaac gaaaacggcg cgtaagtcat acttgagccg tgaaagacaa caattccaac
     3481 ggcaaacccc cgcaggccac caaccggcgc cgtgcgt
//
LOCUS       DATDEU010000585         9308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_845147, whole genome shotgun sequence.
ACCESSION   DATDEU010000585 DATDEU010000000
VERSION     DATDEU010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845147"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VIV63_14025"
     CDS             complement(<1..896)
                     /locus_tag="VIV63_14025"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6125765.1"
                     /translation="MQARLKQIVIAGGGSAGWMTAAALSSLLDPKDVSITLVESDEIG
                     TIGVGEATIPDIIAFNRMLGINEAEFMKATNATFKLGIEFVDWGRKGDRYIHPFSVHG
                     VDMKGIDFHNYWLHEHAGGGQHPIQQYSLCAVAASLGKFTLPDPNPRSLLTYMRYAYH
                     FDATLYARYLRTFAQARGVQRVEGKIQRVSQAPESGHIESLQLDGDRVVRGDFFFDCS
                     GFRSLILGSVLGVPFVDWSHWLPCNSALAVPCRHAGPLLPYTRATARTAGWQWRIPTQ
                     QRTGNGHIYCREFMNDDEAAAML"
     gene            complement(901..2346)
                     /locus_tag="VIV63_14030"
     CDS             complement(901..2346)
                     /locus_tag="VIV63_14030"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY6125766.1"
                     /translation="MQRIIVCGNGLAAHLTAVALARQLPPTIRITWVNGADSRDADLF
                     FGGITAPSAYAFNLAAGVSEPRMMLDTDSTFSWGTHFQDWGAANISWVQCSHLPLPII
                     GGVLFHHHLWRLGIGELEPFLTPAIAARRGVFAHPVDKGPKLLSRAEYGYQFNPYSYR
                     LPFAAAATAFQVEVVTADILEVVCGEGTIFALRLSDGREIEADLFVDCTGPDARLLSR
                     LGVEAPAGRRLRAAYSQRPTERVGPPCRSVVARDFGWQSETSLKGSVARLTVYAPETE
                     SEALSAHGEPQHTGEITLGRRASTWVGNCVAIGHAASVLEPLSHAPMLLLEREIDRLL
                     TLVPFSDEMSVERREYNRQGAEDYAHAHIFNRALYETPPKAQTSFWLAAQAEPPDEKL
                     IHKLTQFESRGLLAAFDLEPFTAEDWVVLHHGMRRRPVRHDRMADRTPVEEVRPMLDS
                     MSREIEKLVKTMPTHHDYMLNLVRYLRQNKS"
     gene            complement(2379..3878)
                     /locus_tag="VIV63_14035"
     CDS             complement(2379..3878)
                     /locus_tag="VIV63_14035"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6125767.1"
                     /translation="MGGGTAGWMTAAALSHKLKGLPVSIHLVESDEIGTVGVGEATLP
                     HIRNFNTALNISEPALMKATEATIKLGIEFCDWGRIGDRYIHPFGDFGPNVNGVPFYQ
                     FWLRLRELGDTSRLHEYSLGVVLAEMGRFTTPGTDPARVDSTFRYAYQFDATRYARFL
                     RQYSEANGVVRIEGKVVDVKLDSMTGHVESVKLDRGETVGADLFVDCSGFRGLLIEQQ
                     LKTGYEDWSQWLPCNRAVAVPCESRGPLLPLTRATARKAGWQWRIPLQHRVGNGLVYC
                     SDFIDDDAATEFILANLEGPALAPPNKLRFTTGRRRLFWNKNVVAIGLAAGFLEPLES
                     TSIHLIHEGITELVQLFPDKGFAQSDADEYNRRMGVNFERVRDFLLLHYVATQRDDSP
                     MWLHFRNLALPDSLREKIDAWTTRGYIIKYEFGVFLPPSWIAVMMGQNLWPRGYDRRA
                     DAAPKEMLIRNAAAIKQGVRAAAQNAPDHAAYLRQIGAASDTGPLVARG"
     gene            complement(3918..4538)
                     /locus_tag="VIV63_14040"
     CDS             complement(3918..4538)
                     /locus_tag="VIV63_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125768.1"
                     /translation="MKRSMGVAAVAAVLWGCASAPPPAPVYEAATQVNSPGYLTAPSE
                     NDRFVVSYTGTRGMSREQVAQFALLRAAEFTAESGKEWFAVITTKTQSVTLVQTKEDL
                     KGRSGGGFIGGPGGSSTAAGGTGAPAASGTADAQTGGGATAGGFGGGAPPTQVLERWT
                     PPKVLQTAIVIQMGAGEKASFAGLDKAPEIFAAKSTADDIRAKMKP"
     gene            complement(4625..7846)
                     /locus_tag="VIV63_14045"
     CDS             complement(4625..7846)
                     /locus_tag="VIV63_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6125769.1"
                     /translation="MAVSSAIAAAQENAQRQNAANAPATGQAAEGDVQTVVVTGIRAS
                     LESAAEIKRRADTFVDSVTASDVTSLPDMSVAEALQRIPGVTVSRFPTTGGASPDFPS
                     PEGRGNLIRGLGFIRSEFNGRDAFSANGGRALDWSSIPPELVGGIDVYKNQSADLIEG
                     GIGGTINLRTLEPFDREDHIVAFSADGTYGDLGENWSPSYAAVVGDRWGTDEAGEFGV
                     LASYSRSKLRSAINGWQQGAPTPRTQVTTAEFPNDFDKSDAYDGLTADDIQAYVPGFQ
                     LRTNDVDRDRTSAYLALQWQKESVRATVKYTRVENSIDSLEHTTEWFPNHSGGNQFTV
                     SDLTMDNSWSTPGIAMCNGDGGRINGTPDNPNGDGQNPGDCETMVPARGLMQSGLVSS
                     QIDSWTGAYGVGVGTLGVGKHENATTEDLSFNLKFNATENLFLEFDAQLTKADADYVE
                     VWGGGTFFSDVFVDPDLESPTVEFRVNPNTGINQSNTRDLGTGRLPVPTSTADPSNAY
                     WLFASDSFREGTGELKAFRADSRYEFGEDSWFKAVRFGLRYSEREQLNKEIGGNWGGI
                     SPAWAGGYGVFSEMTTPAYELVDFKNFFRGGVVQGANSKFPYIRSDLLMNYSAMRNYM
                     LNEPDIGTNHPWAPRGNLDGTGAYRPEDISDITEETQNAYVRFDFGHDIGSNGMSIDA
                     NVGVRFSKSDLSSNGFIAYDDFDVDAPAPVPDPRAPGTEDRDHPRDFLPETSAFLDQA
                     ATPYFVDQSDTEWLPSLNVKWNLNDNSLVRFGASKGLTRPNIQDLRATRLVGATTTRT
                     SFPQITDPNDPLFGVDRGAQDIDLGRVTVTRGNPDLKPTTAVSLDLSYEWYFQGGYLS
                     AALFTKDLKNIITTGDLTLGQVTLDGQTVDLVYNGPINQATADIKGFELAYQQFFDRL
                     PGWMSHLGVQANYTNIQADADPPGMGVDANDDGVPEDVTQAFRFGVRDLLGQSEHIAN
                     LVGIYQDEKMEFRLAYNWRSEYLTTYRDWVSGNPIYNAPSGILDGSFRYDFNEHFQVS
                     ASMSNILDKKEKANMMLNETGQMAPRFAFLNDRRFVLGMRYQF"
     gene            complement(8171..>9308)
                     /locus_tag="VIV63_14050"
     CDS             complement(8171..>9308)
                     /locus_tag="VIV63_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125770.1"
                     /translation="GTAVALSADGRVMAVLARTRQTVSLYRRIGSAWVPDGIAVPPPG
                     FITSSGPTAHHTLALSGDGTVLAVGRPGEILPWPDGASYGATHMYRRMNGNWQFVQKL
                     VPEDRSQYRYFGHVVDMDDAGDWLAVMMMDRTTSRPGEVHLYRTVGGTWVLEKIFPVP
                     WNDLGAYAACGALALSGDGSRLTRQCQRSYPSHQTYVETYTAPAWNAGPELLIANNVT
                     PLRAIDLNGDGTLLATSVANTVTAIWRLTGAGWQPDGPNGSAALIPVSQPNMTMSRNG
                     NYLAFGSSYDSTLGLGVVYPPFSRGLDTGTVRIFERRGGAWPLLRTLKSNSSTQQIFG
                     YSLAFGDNGSVLAVGAPADSSAATGIDGDQYDTSVRGNGAVWLY"
BASE COUNT         1739 a         2888 c         2886 g         1795 t
ORIGIN      
        1 agcatcgccg ccgcttcatc gtcgttcatg aactcgcggc aatagatgtg cccgttgccc
       61 gtgcgctgct gcgtcgggat ccgccactgc cagccggcgg tcctcgccgt ggcccgcgtg
      121 tagggcagca ggggtcccgc atggcgacat ggcaccgcga gcgccgaatt gcacggcagc
      181 cagtggctcc agtccacgaa cggcacgccc aggactgatc ccaggatgag cgaccggaat
      241 ccgctgcaat cgaagaagaa gtcgccgcgc accacccggt cgccgtcgag ctgcagcgac
      301 tcgatgtggc cggattccgg cgcctgcgaa acgcgctgga tcttgccttc gacgcgttgc
      361 acgccgcgcg cctgcgcgaa cgtgcgcagg tagcgcgcgt acagcgtcgc gtcaaaatga
      421 taggcatagc gcatgtaggt cagaagagat cgcggattcg gatccgggag cgtgaacttt
      481 cccaggctcg ctgccaccgc gcacaggctg tattgctgga tgggatgctg gccgcccccg
      541 gcatgctcgt gaagccagta gttgtggaag tcgatgccct tcatgtccac gccatgaaca
      601 ctgaaaggat ggatgtagcg gtcgcccttg cgaccccaat cgacgaactc gatgccgagt
      661 ttgaacgtcg cgttcgtggc cttcatgaac tcggcttcat tgatgccgag catgcgattg
      721 aaggcgatga tgtcggggat ggtcgcttca cccactccga tcgtgccgat ctcatcggac
      781 tcgacgagcg tgatggagac gtctttcgga tcgagcagac tcgaaagcgc agcggcggtc
      841 atccacccgg cggagccgcc accggcaatg acgatctgct tcaggcgcgc ttgcatgagg
      901 tcatgacttg ttttgcctga ggtaccgcac caggttgagc atgtagtcgt ggtgagtcgg
      961 catcgttttc accagcttct cgatttcgcg cgacatcgag tcgagcatcg ggcgcacctc
     1021 ctcgacgggc gtgcggtcgg ccattcgatc gtggcgcacc ggccggcgcc gcattccgtg
     1081 atgcaggacg acccagtctt cggcagtgaa cggttccaga tcgaacgccg ccagcaatcc
     1141 gcggctctcg aactgggtca gcttgtgaat gagcttttcg tccggtggct ccgcctgggc
     1201 cgcaagccag aagctcgtct gcgctttggg cggtgtttca tacagcgcgc gattgaagat
     1261 gtgcgcgtga gcgtagtcct cggcgccctg ccgattgtac tcgcggcgtt ccaccgacat
     1321 ctcgtccgag aacggcacca gggtcagcag ccgatcgatc tcccgttcga gcaaaagcat
     1381 cggcgcgtgg ctcaacggct cgagaaccga ggcggcgtgt ccgatggcga cgcagttgcc
     1441 cacccaggtg ctggcgcggc ggcccagcgt gatttcgcca gtgtgttgcg gttcgccatg
     1501 cgcgctgagc gcctcgcttt ccgtttccgg cgcatagacg gtcagccggg cgacacttcc
     1561 cttgagggaa gtttcagact gccagccgaa atcgcgcgcg accacgctcc ggcacggcgg
     1621 tccgactctt tccgtgggac gttggctgta cgcagcccgc agccgtcgcc ctgccggagc
     1681 ttcgaccccc aggcgtgaaa gcaagcgcgc atccggaccg gtgcaatcga caaatagatc
     1741 cgcctcgatc tctcggccat cggaaaggcg tagcgcgaaa atcgtgccct cgccgcatac
     1801 gacctcgaga atatccgcgg tgaccacttc cacctggaat gccgtagccg ccgccgcaaa
     1861 cggcaagcga taggaataag gattgaactg gtagccatac tccgcgcgcg agagaagctt
     1921 gggccctttg tcgacgggat gtgcgaacac cccacgccga gcggcgatgg ccggagtcag
     1981 gaatggctcg agctcgccga tcccgagacg ccacaggtga tgatggaaca gcacgccacc
     2041 gatgattggc agcggcaggt gcgagcactg cacccacgaa atgttcgctg ctccccagtc
     2101 ctggaaatgc gtaccccagg agaaagtcga gtcggtgtcc aacatcatgc gcggctctga
     2161 aacccctgcc gcgagattga aagcataagc cgagggcgcg gtgatgccgc cgaaaaaaag
     2221 atccgcatcg cgcgaatccg cgccattgac ccaggtgatt cgaatcgtcg gtggcaactg
     2281 gcgggccagc gccacggcgg tcagatgagc ggcgaggccg ttgccgcaga cgatgatgcg
     2341 ctgcacgggt ttcacggctg gagctggctc accggtggct agcctctagc taccagaggc
     2401 ccggtatcgg aagcggctcc gatctgccgg agatacgcgg cgtgatccgg ggcgttctgg
     2461 gccgctgcgc ggactccttg cttgatggcc gcggcattcc tgatgagcat ttccttcggt
     2521 gccgcatccg ctcgccggtc gtaaccccga ggccacagat tctgccccat catcaccgcg
     2581 atccagctgg gcggcaggaa cacgccgaat tcgtatttga tgatgtagcc gcgggtcgtc
     2641 caggcatcga tcttttcccg caggctgtcg ggaagcgcca gattccggaa gtgcaaccac
     2701 atcgggctgt cgtcgcgctg ggtggcgacg taatgcagca gaaggaagtc gcgcacgcgc
     2761 tcgaagttga cacccatgcg ccggttgtat tcgtccgcgt cggattgggc aaaccccttg
     2821 tcggggaaaa gctggacgag ctcggtgatg ccttcgtgaa tcaggtggat cgatgtcgac
     2881 tcgagcggct ccaggaatcc ggctgccagc ccgatcgcca ccacattctt gttccagaaa
     2941 aggcgccgcc tgccggtcgt gaaccgcagc ttgttgggcg gggcgagcgc gggtccttcg
     3001 agattggcca ggatgaattc cgtcgctgca tcatcgtcga tgaaatcact gcagtacacg
     3061 agtccgttgc ccacccgatg ctgcaacgga atccgccact gccaccccgc cttgcgggct
     3121 gtcgcccggg tcagcggtaa cagcggccca cgcgattcgc acggcaccgc gacggcccgg
     3181 ttgcagggca accattggct ccaatcttca tatccggtct tgagctgttg ttcgatgagc
     3241 agaccacgaa agccggaaca atcgacgaac aagtcggcac ccacggtttc gccacggtcg
     3301 agttttacgg actcgacgtg gccggtcatg gagtcgagtt tcacgtcgac gaccttgcct
     3361 tcgatgcgta cgacgccgtt ggcttccgaa tactgccgca agaagcgcgc gtaacgggtg
     3421 gcatcgaact gatatgcgta gcggaacgtg gaatcgacgc gcgcgggatc ggtgccaggc
     3481 gtcgtgaagc gtcccatctc ggcgaggacg acgccgagcg aatattcgtg gaggcgcgag
     3541 gtatccccga gctcccgcaa tcgcagccag aactgataga aggggacgcc gttgacgttg
     3601 gggccgaagt cgccgaacgg gtgaatgtag cgatcgccga tccgccccca atcgcagaac
     3661 tcgatgccga gcttgatcgt cgcctcggtc gctttcatga gcgccggttc gctgatgttc
     3721 agcgccgtat tgaaattccg gatgtgcgga agcgtggcct cgccgacgcc cacggttccg
     3781 atttcatcgg attcgaccaa gtggatggaa accggcaggc ccttgagctt gtgggagagc
     3841 gccgccgcgg tcatccatcc ggccgtgccc cctcccacga tgcatattct ttcgatttgc
     3901 acagcaaatg cggaggccta aggcttcatt ttcgcgcgga tatcgtcggc cgtgctcttc
     3961 gcggcgaaga tttccggcgc cttgtcgagc ccggcgaacg aggctttttc gccggcgccc
     4021 atctggatga cgatcgcggt ctgcagcacc ttcggcggag tccagcgctc cagtacctgc
     4081 gtcggtggcg cgccgccacc gaatccaccg gcggtcgctc caccgccagt ctgtgcgtcc
     4141 gccgtaccgc tcgccgcggg cgcaccagtg ccgcctgctg cggtgctgct gccgccaggc
     4201 ccgccaatga agccgccacc actccggcct ttcaggtctt cctttgtctg cacgagcgtg
     4261 acgctctggg tcttggtggt gatcaccgca aaccattcct tccctgactc cgcagtgaat
     4321 tccgccgcgc gcagcagggc aaactgcgcc acttgttcac gcgacatgcc tcgggtgccg
     4381 gtgtagctga ccacgaaccg gtcgttctcg gacggggccg tcagatagcc cgggctgttc
     4441 acctgggtcg ccgcttcgta aacgggggcc ggtggcggtg ccgaagcgca accccacaac
     4501 acagcggcaa cggccgcgac gcccatgctt cgtttcatgc tggatcctcc tgttggacgc
     4561 atattggaat ggaaagggcg gagacgacat ctccgccctt gcgcaagtcc cgatccgata
     4621 cagctcagaa ctggtatcgc atacccaaga caaaacgacg gtcgttcagg aaggcgaagc
     4681 gcggcgccat ctggcctgtc tcgttcaaca tcatgttggc cttctccttc ttgtcgagga
     4741 tgttcgacat ggaggcgctg acctggaaat gctcgttgaa atcgtaccgg aacgaaccgt
     4801 cgagaattcc gctcggggcg ttgtagatcg gattgccgga gacccagtcg cggtaggtcg
     4861 tcaggtactc agagcgccag ttgtaggcca gccggaactc catcttctcg tcctgataaa
     4921 taccgaccag gttggcgatg tgctcggact gtcccagcaa gtccctcaca ccaaaacgga
     4981 acgcctgggt gacatcctcc ggtactccgt catcgttcgc gtccacaccc atgcccggcg
     5041 gatccgcgtc cgcctggatg ttggtgtagt tagcctgcac gcccagatga gacatccagc
     5101 ccggcaaccg gtcgaagaac tgctggtacg ccagttcgaa gcctttgata tcggccgtcg
     5161 cctggttgat cgggccgttg tacacgaggt cgaccgtctg tccgtcgagc gtaacctggc
     5221 ccagcgtcag atcacccgtc gtgatgatgt tcttgagatc cttcgtgaac aaagccgccg
     5281 acaagtagcc tccctggaaa taccactcgt aggagaggtc caggctgacc gcggtcgtcg
     5341 gcttcagatc gggattacct cgcgtgacgg tgacgcggcc gaggtcgatg tcttgcgcac
     5401 cacgatccac gccaaacagc gggtcattcg ggtccgtgat ctgcgggaaa ctggtgcgcg
     5461 tcgtggtggc acccacgagg cgcgtagcac gcagatcctg aatgttcgga cgcgtcagac
     5521 ccttgctggc gccaaagcgc accaggctgt tgtcgttcag gttccacttc acattgaggg
     5581 acggcagcca ctccgtgtcg ctctggtcga cgaagtacgg ggttgccgcc tgatcgagga
     5641 aagccgacgt ctccggcagg aaatcgcgcg gatggtcgcg gtcttccgta cccggagccc
     5701 gggggtccgg gacaggcgcc ggcgcatcca catcgaagtc gtcgtacgcg atgaagccgt
     5761 tcgatgagag atcagacttc gagaaacgaa cgccgacgtt cgcatcgatc gacatgccat
     5821 tgctgccgat gtcgtgaccg aaatcgaacc gcacgtatgc gttttgagtt tcttcggtga
     5881 tgtcggagat gtcttccgga cgataggcgc cggttccatc caggttgccg cgtggcgccc
     5941 agggatgatt ggtcccgata tccggctcgt tgagcatgta attgcgcatc gcgctgtaat
     6001 tcatcaacag gtcgctgcga atgtacggga acttggaatt ggcgccttgc accactccac
     6061 cacggaagaa gttcttgaaa tcgacgagct cgtaagcggg cgtggtcatc tccgagaaca
     6121 ccccgtagcc gcccgcccaa gccggggaga tgccgcccca gttgccgccg atttccttgt
     6181 tgagctgctc acgctcggag tagcgcaggc cgaaacgcac ggccttgaac cacgaatctt
     6241 ccccgaattc gtagcgcgaa tcggcgcgga acgccttcag ttcgccggtg ccttcgcgga
     6301 aagaatcgga agcaaacagc cagtacgcgt tgctcggatc cgcggtcgaa gtcggaaccg
     6361 gcaaccggcc ggtaccgaga tctcgcgtat tgctctggtt gatgcccgtg ttcgggttga
     6421 cgcggaattc gacggtgggg ctttcgaggt ccggatccac gaacacgtcc gagaagaacg
     6481 tgccgccacc ccagacttcg acgtagtccg catcggcctt ggtcagctgg gcgtcgaact
     6541 ccaggaacag attttcggtg gcattgaact tgaggttgaa gctgaggtct tcggtggtcg
     6601 cgttctcgtg cttgcccacg ccgagcgtac ccactcccac tccgtatgca ccggtccagg
     6661 aatcgatctg gctggacacc aggcctgatt gcatcaggcc acgcgccgga accatcgttt
     6721 cgcagtcgcc ggggttttgt ccatcgccat tgggattgtc cggggtgcca ttgatacggc
     6781 cgccatcgcc gttgcacatc gcgatcccgg gcgtcgacca gctgttgtcc atcgtgagat
     6841 cgctcaccgt gaactgattt ccgccggagt gattcgggaa ccactccgtg gtgtgctcga
     6901 ggctgtcgat cgaattctcg acgcgggtgt acttgaccgt cgcgcggact gattccttct
     6961 gccactgcaa cgccaggtag gcgctggtgc ggtcgcgatc gacatcgttg gtgcgcagct
     7021 ggaagcccgg cacgtaagcc tggatgtcgt cggctgtcag gccgtcgtaa gcatccgact
     7081 tgtcgaagtc attcgggaat tccgctgtcg taacctgcgt gcgcggagtc ggcgcacctt
     7141 gctgccagcc gttgattgcg gagcggagct tcgatcgcga gtaggacgcc agaacaccga
     7201 actcgcctgc ttcgtcggtg ccccaacgat cgccgacaac ggccgcgtat gacggagacc
     7261 aattttcgcc gaggtcccca taggtgccat ccgctgaaaa cgcgacgatg tgatcctcgc
     7321 gatcgaacgg ctccaatgtg cgcaggttga tcgtgccgcc gatgccacct tcgatgagat
     7381 cggcagactg gttcttgtag acgtcgattc caccgacgag ctcgggcgga atgctcgacc
     7441 agtcgagcgc gcggccgccg ttggcggaaa acgcgtcgcg gccgttgaat tcagagcgga
     7501 tgaagcccaa accgcggatc aggttgccgc ggccttccgg ggacgggaag tcaggcgaag
     7561 caccgcccgt cgtggggaaa cgcgaaaccg tcacgcccgg aatgcgttgc aacgcttcgg
     7621 cgaccgacat gtcgggcaga ctcgtgacat ccgaggccgt gaccgagtcg acgaacgtgt
     7681 cggccctgcg cttgatctcc gccgcgctct cgagcgacgc gcggatgccc gtgacgacga
     7741 cggtttgaac atcaccctcc gcagcctggc ctgtggccgg cgcgttggcg gcattctgcc
     7801 gctgtgcatt ttcctgagcc gccgcaatgg cggatgagac cgccatggca atggcggacg
     7861 taccaataag caaacgctcc aactttgatc tggatttcat gcactacccc tctgtcgtgc
     7921 tcttcttgaa ccacataccc cgccgacttc gaaacgagtc attacgggct gaccgcggac
     7981 accgttttat ttggactaga agtgggacac aaataggatt tttgcgatgc ggaatatcag
     8041 ttttactatg gcaacagatt ttcacgcgcg aatctgaccg aattagactc cttcagagcg
     8101 cggctgatcg gatcaaatcc tgccgctgcg atcggcggtt ctgtgcatgc ggggggctac
     8161 gagcgtcacc tcaatacagc cacaccgccc cgtttcccct gaccgacgtg tcgtactggt
     8221 cgccgtcgat gccagtcgcg gcacttgaat cggcgggcgc cccgaccgcg agtacgctgc
     8281 cgttgtcgcc gaatgccagc gagtatccga aaatttgctg agtcgacgaa ttggatttca
     8341 acgtgcgcaa cagcggccat gccccgccgc ggcgttcgaa tatgcgcacg gtaccggtat
     8401 cgaggccgcg gctgaatggt ggatagacca cacccagtcc gagggtgctg tcatatgagc
     8461 ttccgaacgc cagataattt ccattgcggc tcatcgtcat gtttggctgg ctgacaggaa
     8521 tgagcgccgc cgagccattt gggccatcgg gctgccagcc cgcacctgtc agccgccaga
     8581 tcgcggtgac ggtgttggcg actgaggtgg cgagcaaggt gccatcgcca ttgagatcga
     8641 tcgcacgcaa cggagtgacg ttattggcga tgaggagttc cgggcctgca ttccaggccg
     8701 gcgcggtata ggtctccacg tacgtctggt gagaggggta gctgcgttgg cactgacggg
     8761 tcaagcgact tccatcgccg gacaatgcaa gcgccccgca ggccgcgtac gcacccaaat
     8821 cgttccacgg gactggaaag atcttctcga gtacccaggt tcctccgacg gtgcggtaaa
     8881 gatgaacctc acccgggcgc gaggtcgtgc ggtccatcat catgaccgcg agccaatcgc
     8941 ccgcgtcgtc catgtcaacg acatgtccaa agtaccggta ttgggagcgg tcttccggaa
     9001 ctaacttctg cacgaactgc cagttgccgt tcatgcgccg atacatgtga gtcgccccgt
     9061 agctggcgcc atctggccat ggcaggattt ctcccggtct gccgacggcg agaacggtgc
     9121 cgtcgccgct caacgcaagc gtgtggtggg cggtcgggcc cgaactcgta atgaatcctg
     9181 gcggcggaac ggcaattccg tcgggaaccc atgcggagcc aatacgtctg tacaacgata
     9241 ccgtctgtct agtgcgggcg agaacagcca tcacgcgacc gtccgcactg agagcgactg
     9301 ccgtgcca
//
LOCUS       DATDEU010000586         3412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1574380, whole genome shotgun sequence.
ACCESSION   DATDEU010000586 DATDEU010000000
VERSION     DATDEU010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574380"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VIV63_14055"
     CDS             <1..623
                     /locus_tag="VIV63_14055"
                     /inference="COORDINATES: protein motif:HMM:NF019618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEY6125771.1"
                     /translation="DRPRPEGRQMTRVEFITVLRRGLKGLAEESTSGIVADYENYFDD
                     GRRAGRSEEQISVALGDPARLAAELRLEADAASWRKSRTRSAGFRTVAGALALLALDG
                     LLLLPLALFSLLVAAGFVIAALAILYGAFTLTYGLFDDPAGGVLAAFLYGLGRASGGL
                     AGIAVCLLLTGWLADFCARYVLLHRRVLRPGAIRPSTTGSPSHESH"
     gene            610..1281
                     /locus_tag="VIV63_14060"
     CDS             610..1281
                     /locus_tag="VIV63_14060"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEY6125772.1"
                     /translation="MNRTNKLLLLAIGGLAVSASALTVAGALEEKDLAKPAITREIAW
                     DGSESLVVDAPAEVRFVQSAGPGKVLITGASRSVETFRIDGGVLRDERWHTGKPLRIE
                     VHAPKVTRFVVKGGDKLTIESFDQPELHIEATGRADVTAAGRATTLKLILQGSGWADL
                     SGLATDGAEVTVSGSRQALIAPLSWATLSGNGTVVLLTNPPRVDTNFGESGRVIRATP
                     PLASR"
     gene            1374..2084
                     /locus_tag="VIV63_14065"
     CDS             1374..2084
                     /locus_tag="VIV63_14065"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY6125773.1"
                     /translation="MKLVALLAFLLPFTACAADKVATYKTSHPLKIGIIGTGEIGGAL
                     ARHWGAAGHDLLISSRHPEQLQALAKEIGPNVKVGTPREAAAFGEVVLISVPYHATAQ
                     VGRDYAAELKGKVVLDTGNPYPSRDGEMAVRDRQRGTGVASAEYLPGTRLVRAFNAIN
                     SGPLEREAFRKPERLGIPLAADDPSAMKIAEQLVSDAGFDPVPVGKLSRARDFDYGTP
                     VYVRGMTAAELRQQMGLK"
     gene            2308..>3412
                     /locus_tag="VIV63_14070"
     CDS             2308..>3412
                     /locus_tag="VIV63_14070"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY6125774.1"
                     /translation="MRNHTACRWLFTALALIFSAGATAQNAVRISEIHYDNTGTDAGE
                     AIEVSAPAGTDLTGWQIVLYNGTGGATYDTDALSGIVPATCDTRGVLVINYPSNGIQN
                     GSPDGVALVDNSGTLIEFLSYEGVFAGTNGPANGITSTDIGVLQNGTGPVGESLARNA
                     DGTWNGPAASSFGACNDDGTPPPIPEVDTVEISPSTAAISAGGTVAFTAVAFDIDGQP
                     INGVPLIWESADPAIATVSATGVATGVSEGDTAIFASAANGVGGMALLHVDAAPPPSA
                     DFRINEIHYDNLGTDSGEAIEIEGPAGANATGVSIVLYNGNGGVVYNTQALSGLVPAT
                     CGPRGVRVVNYPADGIQNGSPDAIALVSSTGQVI"
BASE COUNT          578 a         1146 c         1088 g          600 t
ORIGIN      
        1 tcgatcgacc gcgtcctgaa ggccgccaaa tgacgcgcgt ggaattcatc acggtcttgc
       61 gccggggcct caagggactt gcggaggaat ccacctcagg aatcgttgcg gactacgaaa
      121 actatttcga tgacggccgg cgcgcgggtc gatccgagga acagatctcc gtcgcgctgg
      181 gcgatcccgc gcggttggct gccgaacttc gcctcgaagc cgacgcggcc tcctggcgaa
      241 agtcgcgcac gcgcagcgcc ggatttcgca cggtcgccgg ggcgctcgcc ttgctcgcgc
      301 tcgacggcct gctgctgttg ccgctggctc tcttttcgtt gctcgttgcc gcgggatttg
      361 tcatcgccgc actcgccatt ctctacggtg cattcaccct gacgtacggg cttttcgatg
      421 atcccgccgg cggtgtgctc gcggcgttcc tgtatgggct cgggcgtgcg tcgggcggcc
      481 ttgccggcat cgcagtctgt ctattgctca ccggctggct cgccgacttc tgcgcgcgct
      541 acgtgcttct ccatcgccgt gtgcttcggc ccggcgccat tcgtccttcg actacaggga
      601 gtccctctca tgaatcgcac taacaagttg ctgctcttgg ccatcggcgg gctcgccgtg
      661 agcgcctccg cgctcacggt ggcgggcgcg ctcgaagaaa aggacctggc gaaacccgca
      721 atcactcgcg agatcgcctg ggacggcagc gagtctctcg tggtcgacgc gccggccgaa
      781 gtccgtttcg tgcagagcgc ggggccgggc aaggtgctga tcacaggagc ttcacgctcc
      841 gtggaaacat tccgcatcga cggtggcgtc ctgcgcgatg aacgctggca taccggcaag
      901 ccgctgcgca tcgaagtcca cgcgccgaaa gtcacacgct tcgtggtgaa ggggggcgac
      961 aagctcacca tcgagagctt cgaccagccc gagttgcaca tcgaagccac cggccgcgct
     1021 gacgttacgg ctgcgggccg cgcgaccaca ctcaagctca ttctgcaggg ttccggctgg
     1081 gccgacctca gtggtctcgc gacggacggc gcggaggtga ccgtgagcgg ttcgcgccag
     1141 gcgctgatcg cgccgctttc atgggccacg ctttccggca acggaacggt cgtgttgctg
     1201 accaatccac cccgagtgga cacgaacttc ggcgaatcgg gtcgcgtgat ccgtgcgacg
     1261 ccgccgctgg cgagccggtg atgagttcat ccgcctcttt cgcgcggcga gcggaacgaa
     1321 actcccggca tgcgtttggc gtccaacgaa aagtctaaaa cagcggggtc cacatgaaac
     1381 ttgtcgcact gctcgccttt ctattgccgt tcaccgcgtg cgcggccgac aaggtcgcca
     1441 cgtacaagac ctcgcacccc ctgaagatcg gcatcatcgg cacgggtgaa atcggtggag
     1501 cgctggcccg ccactggggt gcggccggcc atgacctgct gatctcctcg cgccatcccg
     1561 agcagcttca ggcgttggca aaagagatcg ggcccaacgt gaaagtgggc acgccgcgcg
     1621 aggccgcggc gttcggcgaa gtcgtgctga tttcggtccc gtatcacgcc acggcgcagg
     1681 tgggccgcga ctacgccgcg gagctcaagg gcaaggtggt gctcgacacc ggcaatcctt
     1741 atccctctcg cgatggcgag atggcggtgc gcgaccgcca gcgcggcacg ggcgtggcgt
     1801 cggcggaata tctgcccggc actcgcctgg tgcgcgcgtt caatgcgatc aattcgggcc
     1861 cgctcgagcg tgaagcgttc cgcaaacccg agaggctcgg aattccgctc gccgccgacg
     1921 atccgtcggc catgaagatt gccgaacagc tcgtgagcga tgccggtttc gaccccgtgc
     1981 ccgtgggcaa actatcgcgc gcgcgtgact tcgactacgg cacgccggtg tacgtgcgcg
     2041 gcatgactgc cgccgaattg cgtcagcaaa tgggtcttaa gtaaattctc gcgaacacct
     2101 aacaatctcc ccgggaatcg cgttttctta acctaacgcg aaaatccccg cggaccggcg
     2161 cgcgggttcg atggtttccc gccgcgccgc gcgttatgtg attttccctg tcacgaaagt
     2221 gtttcagggt ttggcgtatt cttccgcgcc gcgggccttt gggccccatt gcttgcttcg
     2281 cagtcgaaaa tcacagggac ggaaagtatg cgaaaccata ccgcttgccg ctggctgttc
     2341 accgctctcg ccctcatttt ctccgccggt gccaccgcac aaaacgcggt ccgcatcagc
     2401 gagatccact acgacaacac cggcaccgac gccggtgaag ccatcgaggt ctcggcgccc
     2461 gcgggcaccg acctcaccgg gtggcagatc gtgctgtaca acggcacggg cggcgccact
     2521 tacgataccg acgcgctgtc gggaatcgtt cccgcgacct gcgacacgcg cggcgtgctg
     2581 gtcatcaact atccgtcgaa tggaatccag aacggctcgc ccgacggcgt cgcgctggtc
     2641 gacaacagcg gcacgctgat cgaattcctg tcgtacgaag gcgtgttcgc gggcaccaac
     2701 ggtcccgcga acggaatcac gtcgaccgac atcggggttc tgcagaacgg caccggcccc
     2761 gtcggcgaat cgctggcgcg caacgccgat ggcacctgga atggtccggc cgccagctcc
     2821 ttcggcgcct gcaacgatga cggcacaccg ccgccgattc ccgaagtcga cacggtagaa
     2881 atctcgccat cgaccgcggc catcagcgcc ggtgggaccg tggctttcac ggcagtagct
     2941 ttcgacatcg acggtcagcc catcaacggc gtgccgttga tctgggagag cgccgatccg
     3001 gcgatcgcca cggtgagcgc caccggcgtc gccacgggtg tctccgaagg tgatacggcc
     3061 atctttgcca gcgcggccaa tggcgtgggc ggcatggctc tgttgcacgt cgacgcggcg
     3121 ccgccgccga gcgccgactt ccgcatcaac gaaatccact acgacaacct cggcacggac
     3181 tcgggcgaag ccatcgagat cgaaggcccc gccggcgcga acgcgaccgg cgtgtcgatc
     3241 gtcctctaca acggtaatgg cggcgtggtc tacaacacgc aggcgctcag cggcctggtg
     3301 ccggcgacct gcggtccgcg cggcgtgcgg gtggtgaact acccggccga cggcatccag
     3361 aacggatcgc ccgacgccat cgcgctggtg agctccaccg gccaggtgat cg
//
LOCUS       DATDEU010000587         5076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1721091, whole genome shotgun sequence.
ACCESSION   DATDEU010000587 DATDEU010000000
VERSION     DATDEU010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721091"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VIV63_14075"
     CDS             <1..638
                     /locus_tag="VIV63_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY6125775.1"
                     /translation="TAMRALGVKLIEHGHDFQESREEAARVGARDGLHMVPAFHPDIV
                     LGVTSYWAELFRAAPDLDVVYAPIGQGSGICAAAAARNVYSPRTRIVGVVSAHARTFS
                     KSFAAKQIVESPVETKLGDGMACRKPDPEALAIVLANVSRVVEVTDAELASAMRAIFA
                     MTHNVAEGAGAAAFAAAWRERESLAGLRVGITVSGGNVDTDVFADVLAGKY"
     gene            647..976
                     /locus_tag="VIV63_14080"
     CDS             647..976
                     /locus_tag="VIV63_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HEY6125776.1"
                     /translation="MKIADLPFGTVDWSAVESTTHAGESGEAHWRTRHFGDVRVRMLD
                     YSAGYLADHWCTKGHFMYCLEGELETELADGRRFTLRPGMSYHVADGAEPHRSSTRTG
                     ARLYVVD"
     gene            963..1412
                     /locus_tag="VIV63_14085"
     CDS             963..1412
                     /locus_tag="VIV63_14085"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6125777.1"
                     /translation="MSSTETAPGAYEISTDPSRLDVQAIHAYLTRSYWSPGIPLDTVE
                     RAVRHSLCFGVYEKSSGAQVGFARVVTDHATFAYLCDVYVLEAHRGDGIGKRMMQEVM
                     AHPALMGARRVMLATRDAHGLYRQSGFAEAGREGNLMEIVRPHMYAA"
     gene            1448..2848
                     /locus_tag="VIV63_14090"
     CDS             1448..2848
                     /locus_tag="VIV63_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY6125778.1"
                     /translation="MNFSKSIGWPVAAGALIALSACGSTPTREKPLKEIVPLTAPVLA
                     PGVESNSDRLFVVVTFSGGGKRAAAFSFGVLEALRDVKVTTPDGEQHSLLDEIDLISA
                     VSGGAFTAAYYALNGKEAFDSYAPRFLNHDTEKDLWHKILKPTNWPRLAKPLVSRSDL
                     EVEYFDEELFNGATVYDVMTRSPRVIVSATDLVRAKPFAFTREQLNGICVDPKSVPVA
                     RAVFASAATPIYFAPLVMRTFAGQCGYQPPDNVESDAAGARDVYRKERADRLLSFLDS
                     KNAPYLHLADGAFADNLGARAILDEVALGDTVTDALRRNGFSRARRMLFIVVDARTGF
                     DETYAQQPTPIGIKDVMDAVVSVTFNRYSFETMNLMRNRVKTWETEVRSTRCKVALAI
                     PDCDKFDVDLVELSFERVKEPAEREYLDRVPTAFTLSSEQIIRLRRAAHLLVDESAEL
                     RVFLEESAKSADKSRK"
     gene            2919..3905
                     /locus_tag="VIV63_14095"
     CDS             2919..3905
                     /locus_tag="VIV63_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6125779.1"
                     /translation="MRPIRRILVAVKNPAARALPAVNKAAQIAKGLNAHLTLFHDIAT
                     PIYAEALQGRDFNLQSWQREVQTARREQLEKLAARIRKHGIAVDVAADWDYPPYEAII
                     RKAQRLGADLLVVENHHGTGRHPARWLLAYTDWELLRTCPIPVLLVKNRRLYHRPRVL
                     AAVDPSHAFAKPTNLDRQILRAGAQLVHALRGELHALHVFHPGVPIVPAMPDGPMLDV
                     AAERDLERQARRKLERVVDGFDVKRSHCHLVEGRPNEAIPVAARKERSAIVAMGVVSR
                     SGLKRFFIGNTAEFVMDAVTSDILVVKPADFDSRVPRTSRGVQILSMPILPG"
     gene            4099..4323
                     /locus_tag="VIV63_14100"
     CDS             4099..4323
                     /locus_tag="VIV63_14100"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY6125780.1"
                     /translation="MRKRAPRKPAARENKVTQARFMGPELRSALIAEAAFFRAEKRGF
                     APGNEVEDWLAAESEVDARLMSAKSAGKPA"
     gene            4374..>5076
                     /locus_tag="VIV63_14105"
     CDS             4374..>5076
                     /locus_tag="VIV63_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6125781.1"
                     /translation="MTAPTPEVLALLEATFDAVVIMDERGRIEAFNASADEMFGYRAA
                     DTIGRNVNMLMAVSDRAGHEDHLERYLADGDARVPGRGRDVRVQHRDGSEFSVFLGVR
                     RIPNSSPPRFVGFLHDTSLRRKALATLEKERQVNRLYLDLAQVMLVATDRNGVVQLAN
                     QRAIRVLKREHAAIVDSNWLESCVAPDDRSTARTAFRALLMSGDDEPQVCEYHVRSSD
                     GEDRFITWRGVALRDI"
BASE COUNT          915 a         1710 c         1597 g          854 t
ORIGIN      
        1 acactgcgat gcgcgcgctc ggcgtgaaac tcatcgagca cggacacgat ttccaggaat
       61 cacgcgaaga agccgcgcgt gtcggcgcgc gcgacggtct gcacatggtg ccggcgttcc
      121 atcccgacat cgtcctcggt gtcaccagct actgggcgga actgttccgc gcggcgcctg
      181 acctcgatgt cgtctacgcg cccatcggcc agggctccgg gatctgcgct gccgcggcgg
      241 cgcgcaacgt gtattcgccg cgcaccagga tcgtcggcgt ggtgtcggcg cacgcgcgga
      301 ctttctcgaa gtcgttcgcg gcgaagcaga tcgtcgagtc ccccgtggaa acgaaactgg
      361 gcgatggcat ggcgtgccgc aaaccggatc ccgaagcact ggctattgtc cttgccaacg
      421 tgagccgcgt ggtggaagtc accgatgcgg aactcgccag cgcgatgcgc gccatcttcg
      481 cgatgacgca caacgtcgcc gaaggcgccg gcgccgcggc attcgcggcc gcctggcgtg
      541 aacgcgaatc tctggccggt ctgcgcgtcg gcatcacggt gagtggcggc aatgtcgaca
      601 ccgatgtgtt tgccgacgtg ctcgccggca agtactgact atcgtcatga agatcgccga
      661 cctgcctttc ggcaccgtcg actggtccgc cgtggaatcc acgacccatg cgggcgagtc
      721 gggcgaggca cattggcgca cgcgtcactt cggcgacgtg cgcgtacgca tgctcgacta
      781 ttccgccggc tatctcgccg accactggtg cacgaaggga cacttcatgt actgcctcga
      841 aggcgaactc gaaaccgaac tcgccgatgg ccgccgattc acgttgcggc ccggcatgag
      901 ttatcacgtc gccgacggcg ccgagccaca ccgctcttcc acccgaaccg gcgcgagact
      961 gtatgtcgtc gactgaaacc gcccccggcg cctacgagat ctccaccgat ccatcacggc
     1021 tcgatgtgca ggcgattcac gcctatctca cgcgttcgta ctggtcgccc ggaattccac
     1081 tcgacaccgt cgagcgcgcc gtgcggcact cgctgtgctt cggcgtgtac gagaaatcat
     1141 cgggcgcgca ggtgggcttc gcccgcgtgg tcaccgacca cgcgacgttt gcctatctgt
     1201 gcgatgtcta tgtgctcgaa gcgcatcgtg gcgacggaat cggcaagcgc atgatgcaag
     1261 aggtcatggc gcatccggcg ctcatgggtg cgcgccgcgt catgctcgcc acgcgcgacg
     1321 cgcacggact ctatcgccag tcagggttcg ccgaagcggg acgcgagggc aatctcatgg
     1381 agatcgtgcg gccgcatatg tacgcggcat aacgggttcc tgcggatcgc gccgtatact
     1441 gcggtgcatg aatttctcca aatcgattgg gtggcccgtg gccgccggcg cgctgattgc
     1501 gctctccgcg tgcggcagca cgcctacccg cgagaagccg ctcaaggaaa tcgtgccgct
     1561 gacggcgccc gtgctcgcac cgggcgtcga atccaactcc gacaggctgt tcgtcgtcgt
     1621 cacgttttcc ggcggcggca aacgggcggc cgcgttttca ttcggtgtgc tggaagccct
     1681 gcgcgacgtg aaggtgacga cgcccgatgg cgagcaacac tcgctgctcg acgagatcga
     1741 cctcatctcc gcggtgtcgg gcggcgcgtt cacggctgct tactacgcgc tgaacggcaa
     1801 ggaagcgttc gacagctacg cgccgcgatt cctcaatcac gacacggaga aggatctctg
     1861 gcacaagatc ctgaagccga cgaactggcc acgcctggcc aagccgctcg tgagccgaag
     1921 cgatctcgaa gtggagtact tcgacgaaga gctcttcaac ggcgccacgg tgtacgacgt
     1981 catgacgcgc tcgccgcggg tcatcgtgtc ggccaccgac ctggttcgcg ccaaaccctt
     2041 tgcgttcact cgcgagcagc tgaacggaat ctgcgtggat cccaaaagcg tgccggtggc
     2101 ccgcgcggta ttcgcgtcgg cggcgacgcc tatctatttc gcaccactgg tgatgcgcac
     2161 gttcgccgga caatgcggct accagccgcc cgacaacgtg gaatcggacg ccgccggcgc
     2221 acgcgacgtc taccgcaagg aacgcgccga ccggctgctg tcgttcctcg acagcaagaa
     2281 tgcgccctac ctgcacctcg cggatggcgc gtttgcggac aacctgggcg ctcgcgccat
     2341 tctcgatgaa gtcgcgctgg gcgacaccgt gaccgatgcg ctgcgccgca acggcttctc
     2401 gcgcgcacgc cgcatgttgt tcatcgtggt ggatgcgcgc accggcttcg acgagaccta
     2461 cgcgcagcag ccgacgccga tcggcatcaa ggacgtcatg gacgccgtgg tcagcgtcac
     2521 gttcaaccgc tacagcttcg aaaccatgaa cctgatgcgc aatcgcgtga agacctggga
     2581 gaccgaggtg cgcagcacgc gttgcaaggt ggcgctggcg atccccgact gcgacaagtt
     2641 cgacgtcgac ctcgtcgaac tgtcgttcga gcgagtgaag gaacccgcgg agcgtgagta
     2701 tctggaccgg gtcccgacgg cgttcacgtt gtcctccgag cagatcatcc ggctacgccg
     2761 tgccgcccac ctgctcgtcg atgaatccgc ggagcttcgc gtatttctcg aggagtccgc
     2821 gaaatcggcg gacaagagcc gcaagtaggc gcgcactgac gacggtatcc gtaatttccc
     2881 ggatgcagta accgcatcgg gtgggcgacg ctcgtggcat gcgaccgatc cgccgaatcc
     2941 tggttgccgt caagaatccc gccgcgcgcg cgttgccggc ggtgaacaag gctgcgcaga
     3001 tcgcgaaggg cctgaacgcc catctcaccc tgtttcacga catcgccacg cccatttacg
     3061 ccgaggcact ccagggtcgc gatttcaatc tgcaatcgtg gcaacgggaa gtgcagacgg
     3121 cgcggcgcga acagctcgaa aaactggcgg cccgcatccg caagcacggc atcgccgtcg
     3181 acgtcgcggc cgactgggac tatccaccgt acgaagccat catccgcaag gcgcaacggc
     3241 tgggcgccga cctgctggtg gtcgaaaacc accatggaac cggccggcat cccgcgcgct
     3301 ggctgctggc ctatacggac tgggagttgc tccgcacgtg tcccatcccg gtgctcctgg
     3361 tgaagaaccg ccgtctctat caccggccgc gcgtgctcgc cgcggtcgat ccctcccacg
     3421 cattcgccaa gcccactaat ctcgaccggc agatcctgcg cgcaggcgcc cagctggtgc
     3481 acgcgctgcg gggcgagttg cacgcgctgc acgtgttcca cccgggcgtc cccatcgttc
     3541 ccgccatgcc ggacggaccc atgctcgacg tcgccgccga gcgtgatctc gagcgacagg
     3601 cgcgccgcaa actcgaacgg gtcgtcgacg gattcgacgt gaagcgcagc cattgccacc
     3661 tggtcgaagg ccgacccaac gaggcgatcc ctgtcgccgc cagaaaggaa cgcagcgcca
     3721 tcgtcgccat gggcgtggtg tcgcggtccg ggctcaaacg ctttttcatc ggcaacaccg
     3781 cggagttcgt catggacgcg gtgacctcgg acatcctcgt ggtgaaaccg gccgatttcg
     3841 attcgcgcgt accgcgcacg agtcgcggcg ttcagatcct gtcgatgccg atcctgccgg
     3901 gctgatgccc ggacccgtgg ggtggttagt ccctggcatc acacacatga aaaaatctcg
     3961 tgctcaaatc gataagaccg cgcgcaatgg tcacgtcgag cacggatact cgcgcatgtg
     4021 cctgttctga aaagcattcg tacaaacccc gaggagtgaa gcacgatgcc acgtgagaaa
     4081 agtccggccc cctcgaccgt gcgaaagcgc gcaccccgca aaccggccgc ccgcgagaac
     4141 aaggtgaccc aggcccggtt catgggaccc gagctgcgct cggcgctgat cgccgaggcc
     4201 gcgttcttcc gcgccgagaa gcgcgggttt gccccgggca acgaggtcga agactggctg
     4261 gcggccgaat ccgaggtcga cgccaggctg atgtccgcga aatccgcggg taaacccgcc
     4321 taaccagtcg cctgctcact gcctgccccg cgcaaggagt atctttgcgg ggcatgaccg
     4381 cgccgacacc cgaagtactc gcactcctgg aggctacgtt cgacgccgtc gtcatcatgg
     4441 acgagcgtgg acgcatcgag gcgttcaacg cctcggccga cgaaatgttc ggctatcgtg
     4501 ccgccgacac catcggccgc aacgtcaaca tgctgatggc cgtttccgat cgcgcgggcc
     4561 acgaagatca tctcgagcgc tacttggccg acggtgacgc ccgtgttccg ggtcgaggcc
     4621 gggacgtgcg cgtccagcac cgggacggca gcgaattttc cgtgttcctg ggcgtcagac
     4681 gcattccgaa ctcgtcaccg ccgaggttcg tcgggtttct gcacgacact tcgctgcgcc
     4741 gcaaggcgct cgcgaccctc gaaaaggaac gacaggtcaa tcgcctgtac ctggacctgg
     4801 cccaggtgat gctggtggcg accgatcgca acggcgtggt tcaactcgcc aatcaacgcg
     4861 ccatccgggt actcaagcgc gaacatgcgg ccatcgtcga cagcaactgg ctcgaaagct
     4921 gcgtggcgcc cgacgaccgc tccacggcgc gcacggcgtt ccgcgcgctg ctgatgagcg
     4981 gcgacgacga accgcaggtc tgcgaatacc acgtgcgctc ctccgacggt gaagaccgct
     5041 tcatcacctg gcgcggggtg gcgctgcgcg acatca
//
LOCUS       DATDEU010000588         2055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_700478, whole genome shotgun sequence.
ACCESSION   DATDEU010000588 DATDEU010000000
VERSION     DATDEU010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700478"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VIV63_14110"
     CDS             complement(<1..898)
                     /locus_tag="VIV63_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY6125782.1"
                     /translation="MNEPSQARLAGHPNQLPRPRDELEKLEAAWELPRGWRIVTAVNN
                     TVIGYFYVAAALLFFLLAGILALLMRLQLAVPGNTFLSPETYNQIFTMHGTMMMFLFA
                     VPVVEAMGILLLPQMLGARDLPFPRLGAFAFWAYFVGGIIFFGTLFYDLAPSGGWSMY
                     PPLTGAQYSPGLGADFWLLGIGFIEISAIAGAIELIIGVLRTRAPGMTLDRMPMFAWT
                     MLVFAGMIVFAFPAVILATLLLEFERAFGWPFFMPQKGGDALLWQHLFWFFGHPEVYI
                     IFLPALGMVSSIVVANTRRPIFG"
     gene            complement(895..1821)
                     /gene="coxB"
                     /locus_tag="VIV63_14115"
     CDS             complement(895..1821)
                     /gene="coxB"
                     /locus_tag="VIV63_14115"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY6125783.1"
                     /translation="MNDMLQSVLAPHGPQAGQIATLAWALFAFGLVILGLVMAATWLA
                     MRGAPRVREALARERTIVMLGLALPVVTLTILLGYGIWLTRAHTGHAADALRIEVTGE
                     QWWWRVVYDGPTPLVTSNEIRIPVGQPVDFALKAADVIHSFWVPSLGGKVDMIPGRVT
                     RLRLTAERAGVYRGQCAEYCGGPHALMAIKIIAMQRHEYEAWFAHALEPPAARSTDME
                     RRGHSLFMAAGCGSCHAVRGTEAAGTIGPDLTDFAARGSVGIDTLPLTAENVAGFIRE
                     GQHIKPGNRMPEFRIFSPAEQTALASYLMSLR"
     gene            complement(1818..>2055)
                     /locus_tag="VIV63_14120"
     CDS             complement(1818..>2055)
                     /locus_tag="VIV63_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6125784.1"
                     /translation="IPACMGCHGEEALKAYPRLARQNLSYMLNRLRLWKNGLGPATAT
                     ETIMVPIARALSQSQIEDVAAYFASGDFPASARR"
BASE COUNT          412 a          651 c          670 g          322 t
ORIGIN      
        1 agccaaagat ggggcgtcgc gtgttggcca cgacgatgga agagaccatg cccagcgccg
       61 gaaggaagat gatgtagacc tccgggtgac cgaagaacca gaacagatgc tgccagagca
      121 gggcgtcacc acccttctgc ggcatgaaga acggccagcc gaaggcgcgc tcgaactcga
      181 gcagcagcgt tgccaggatt accgcaggaa acgcgaacac gatcatcccg gcgaagacca
      241 gcatcgtcca ggcgaacatc ggcattcggt ccagcgtcat gcccggtgca cgcgtgcgca
      301 gcacgccgat gatcagctcg atcgcgcctg caatcgcgga gatttcgata aagccgatgc
      361 cgagcagcca gaaatccgcg ccgagccccg gcgaatactg cgcgcccgtg agcggcggat
      421 acatggacca gccacctgac ggcgcaagat cgtagaacag cgtgccgaag aatatgattc
      481 ctccgacgaa gtacgcccag aacgcgaagg cgcccaggcg gggaaacgga aggtcgcgcg
      541 cgcccaacat ctgcggcagc agaaggatgc ccatcgcttc gacgaccggc acggcaaaca
      601 ggaacatcat catggtgccg tgcatggtga agatctgatt gtaggtctcg ggcgacagga
      661 aggtattgcc gggcactgcg agctggagtc gcatcaggag cgccaggatc cccgcgagca
      721 ggaagaacag cagcgcggca gccacataga agtaaccgat gaccgtgttg tttaccgcgg
      781 tgacgatccg ccaccccctg ggcagttccc aggccgcctc gagcttttcc agttcgtccc
      841 tgggccgcgg cagctggttc ggatgccctg caaggcgcgc ttgtgacggc tcgttcatcg
      901 caggctcatg agatagcttg cgagtgcggt ctgttcagcc ggcgagaaga tgcgaaattc
      961 gggcatgcgg ttgcctggct tgatgtgttg accctcgcgg atgaagcccg cgacattttc
     1021 agccgtgagc ggcaacgtgt cgatcccgac ggaaccgcgc gccgcgaaat cagtcaggtc
     1081 cggtccaatt gtccccgccg cttccgtgcc ccgcacggca tggcagctgc cgcaaccggc
     1141 cgccatgaaa agagagtgac cgcgccgctc catgtcagtc gagcgagcgg ccggcggttc
     1201 gagcgcatgt gcaaaccatg cttcgtattc gtgccgctgc atggcgatga tcttgattgc
     1261 catcagcgcg tgcgggccgc cgcagtactc cgcgcactgc ccgcgataca cgcctgcgcg
     1321 ttcggcggtg agccgcaggc gcgtgacccg gccgggaatc atgtcgacct tgccgcccag
     1381 gctcggcacc cagaagctgt ggatcacgtc ggccgccttg agcgcgaagt cgaccggctg
     1441 tcccacgggg attcggattt cgttcgaggt caccagcggc gtcggaccat cgtaaacgac
     1501 ccgccaccac cattgctcgc cggtcacctc gatgcgaagg gcgtcggccg catgtccggt
     1561 atgggcacgc gtaagccata ttccatagcc gagcaggatc gtgagcgtga cgaccggcaa
     1621 tgccagcccc aacatcacga tggtgcgctc gcgggcgagc gcctcgcgca cgcgcggcgc
     1681 gccgcgcatg gcgagccagg tcgctgccat gaccagcccg aggatgacca gaccgaatgc
     1741 gaacagcgcc caggcaagcg tggcgatctg tccggcctgc gggccgtgcg gcgcaagcac
     1801 ggactgaagc atgtcgttca tcgccgggca ctcgcgggaa aatcgcccga agcgaaatag
     1861 gccgccacgt cttcgatctg cgactgactc aacgcgcgcg cgatcgggac catgatggtt
     1921 tcggtggccg tggctggccc gaggccgttt ttccagagcc gcagccggtt gagcatgtag
     1981 gacaggtttt gtctggcaag gcgagggtag gccttcaggg cctcttcgcc atggcagccc
     2041 atgcaggccg gaatt
//
LOCUS       DATDEU010000589         6396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1139604, whole genome shotgun sequence.
ACCESSION   DATDEU010000589 DATDEU010000000
VERSION     DATDEU010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139604"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VIV63_14125"
     CDS             <1..441
                     /locus_tag="VIV63_14125"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY6125785.1"
                     /translation="RSDDSLGQSPLRSPSVFNFYRPGYVPPNTAIATAGKQAPEFQLL
                     NETTTAGYINFMQGMTRNGYYNFDVNPTYTALLLIAHDVPAVIAWLNLRLTANQLSAN
                     TLSVLQATLSAFNVTASSGANLKLDMLATACFLILCSPEYLVQK"
     gene            438..1835
                     /locus_tag="VIV63_14130"
     CDS             438..1835
                     /locus_tag="VIV63_14130"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY6125786.1"
                     /translation="MSNELWTRRVVLQRAMQLGVMGVATPLAINLAAISEAAAFDNTD
                     YKALVCLFMYGGNDYANTVIPYDNTNYNLYHAIRGGAAGQMAGGIAYGRAQLAATALV
                     PNGGPVLTDNLQYALAPQLAGLKSIWDAGRLAVQLNVGPLIQPTTLAQYQSTNRVQNP
                     LPPKLFSHNDQQSVWQANGTEGSTVGWGGRLGDIAMSSNTNNSLLTCISASGNAVFVA
                     GREALQYQISPAGAIRIAALNTPLRDALSSIITRQSTHVLENEYAIVTRRSIDMEGVV
                     NGALTGVDPLTSFVTTGNPLSSQLKIVARLIAARATLGMRRQVFFVSLGGFDNHNLLM
                     QDHPNLMTRVNDAMSAFYAATSTLGVADKVTLFTASDFGRTLSSNADGSDHGWGSHHF
                     VMGGAVNGGRFYGTAPHVSIQTPDQVGQGRLLPTTSVDEFAATLARWFGCNTGELPGI
                     LPNVANFTNTNLGFV"
     gene            complement(1878..3047)
                     /locus_tag="VIV63_14135"
     CDS             complement(1878..3047)
                     /locus_tag="VIV63_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6125787.1"
                     /translation="MPALQTLAVANYRSIRALVMPLGQLNLITGANGSGKSSLYRALR
                     LLAEAAQGGIVSSLAREGGLSSTLWAGPETISRAMKQGTVAVQGGRRKEPVHLRLGFQ
                     GEHVGYAIDLGLPIPSLSAFTQDPEIKRECVWGAPALRPSSLLADRRGPAVRLRRADG
                     EWDESVARLPTFESMLSVIADPDRAPELLALREEVRSWRFYDHFRADSDAPARHTQIG
                     TRTPVLAHDGRDLAASLQTIREIGDRPALDAAVEDAFPGARVDIEHGGGQFTVLFTQH
                     GLLRPLTAAELSDGTLRYLLWIAALLTPRPPSLMILNEPETSLHPDLLPALARLIEKA
                     STRTQVWVVSHASRMIAALKEARNCNAITLTKELGETAIEGQGMLDAPPWKWPER"
     gene            complement(3191..4120)
                     /locus_tag="VIV63_14140"
     CDS             complement(3191..4120)
                     /locus_tag="VIV63_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125788.1"
                     /translation="MFKFVALAASLLFATAASAQQSLQSLYTRVPAPPATAAAAPAWL
                     ASPELATLRKQVKDQRAFIEKLSREAGAAAQPTAAQTGGGIDFQRAQRDPAYADQVKA
                     KIAAMSQEEQMKLGMQMAQAQSQNALKDVQAMAADPDEVQTAADHYADYQTKQMTSGG
                     ITAQYTAVGKIQQDVNTRAMQISDKAVKALKCSDGEGSCAAADQAADKATLRAAFNQI
                     IAEYDKALPAIAQQVEAARKSRLADIAAAQRDLGPAEYGAAAQSSTNRQLLAAYHNAA
                     LMEIEQLLALSEDAAKWANVRFKDRSINFNKID"
     gene            4296..5456
                     /locus_tag="VIV63_14145"
     CDS             4296..5456
                     /locus_tag="VIV63_14145"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY6125789.1"
                     /translation="MSLTRLNPVRFVMTFALAMLFGAANASEQPPTLAIGAAMPPFSL
                     PGIDGKTYTDKSFAKADILVMVFTCPHCPTAQAYQERIKKLVTDYAPRNVTLIAINPN
                     HADAVRLDELAYSDLSDSFEEMQERARDQKFNFIWLDDGPKQEMSHLYGPVATPHVFI
                     FDKARKLRFEGRIDDSEREAGATTHDTRKALDALLAGKEPPITTTKVFGCSVKWADKA
                     PDYANYRKKWAAEPVTLEKADAAVLKAIRENKDSGKIRFINVWATWCGPCITEFDELV
                     EHNLRFRIRGFEMVTIAAEYPDQEAQVKDFLVKKHASMKNYIFATDKKDELSEALDPA
                     WKGPLPYTIIVDEKGNVIYREMGSINFLKMRRAIVPALNRLTPWKGLSPVKP"
     gene            5509..6045
                     /locus_tag="VIV63_14150"
     CDS             5509..6045
                     /locus_tag="VIV63_14150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6125790.1"
                     /translation="MRIPILASAAAVSGAADEDIAALARQYGPIVFRAAWRVLGGRAA
                     AEDVQQQVFLRLIESPPRNVESWPAFLTTASTRLAIDHLRRQRRWRLLAPLWKADEPD
                     DSPAEDAERAEAGRFMREALARLNPRDASCFVMRHLHGLAPAAIGSALGISENHVSVS
                     VHRARKALEAFQDKEQSR"
     gene            6042..>6396
                     /locus_tag="VIV63_14155"
     CDS             6042..>6396
                     /locus_tag="VIV63_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125791.1"
                     /translation="MNSLDSTLGLFAASEPDAQVVSDAQRKLETVVSKRMAEISARRP
                     ARTARGWLAAGATAAVVAVAMLWLPLGTTPALAFAKVQEHFRDFRTLRFVVEQRMNGE
                     VVMKARVSMVRDGSVR"
BASE COUNT         1123 a         2056 c         2073 g         1144 t
ORIGIN      
        1 cgcagcgacg attcgctagg ccagagcccg ctgcgttcgc cgtcggtgtt caacttctac
       61 cgtcccggct atgtgccgcc caacaccgcg atcgccacgg caggcaaaca ggcgcctgag
      121 ttccagctgc tgaatgaaac cacgaccgcg ggttacatca acttcatgca gggcatgacg
      181 cgcaacgggt actacaactt cgatgtgaat ccgacgtaca ccgcgttgct gctgatcgcg
      241 cacgacgtgc cggcggtgat cgcctggctg aacctgcggc tcacggcgaa ccagttgtcg
      301 gccaacactt tgtccgtgct tcaggcgacg ctgagcgcgt tcaacgtgac ggccagcagc
      361 ggtgcgaacc tgaaactcga catgctggcc acggcgtgtt tcctcatcct gtgctcgccc
      421 gaatacctgg tgcagaaatg agcaacgaac tctggacccg ccgcgtcgtt ctgcaacgtg
      481 cgatgcaact cggcgtgatg ggcgtcgcca cgccgctcgc gatcaacctc gcggcgatca
      541 gcgaagccgc ggccttcgac aacaccgact acaaggcgct ggtgtgcctg ttcatgtacg
      601 gcggtaatga ctacgccaac accgtcatcc cgtacgacaa cactaactac aacctgtatc
      661 acgccattcg cggcggggcc gcgggccaga tggcgggcgg tatcgcctac ggccgcgcgc
      721 agctggctgc cacggcgctc gtgccgaacg gcggacctgt tctcaccgac aatctgcaat
      781 acgcgctcgc gccgcaattg gccggactca agtcgatctg ggatgcgggg cgtctggccg
      841 tgcagctcaa tgtcggcccg ctgatccagc cgacgacgct ggcgcaatac cagagcacga
      901 atcgcgtaca gaatccgctg ccgccgaagc tgttttcgca caacgatcaa caatccgtat
      961 ggcaggcgaa cggcaccgaa ggctcgacgg tgggttgggg cgggcggctc ggcgacatcg
     1021 ccatgtcgag caacaccaac aattccttgc tcacctgcat ctcggcgtcg ggcaacgcgg
     1081 tgttcgtcgc gggacgcgag gcgctgcagt accagatcag tccggcgggt gcgatccgca
     1141 tcgccgcgtt gaacacgccg ttgcgcgatg cgctgagctc gatcatcacg cggcaaagta
     1201 cccacgtgct cgagaacgaa tacgccatcg tcacgcggcg ttcgatcgac atggagggcg
     1261 tggtcaatgg cgcgctcacg ggcgtggacc cattgacgtc ttttgtcacc acgggaaacc
     1321 cgctttccag ccagctcaag atcgtggcgc ggctcatcgc cgctcgcgcc acgctcggca
     1381 tgcgccgcca ggtgttcttc gtatcactcg gcggtttcga taatcacaac ctgctgatgc
     1441 aggatcatcc caacctgatg acgcgcgtga acgacgcgat gagcgcgttt tatgccgcga
     1501 ccagcacgct cggcgtggcc gacaaggtca cgctgtttac cgcgtcggac ttcggccgaa
     1561 ccttgagctc gaacgccgat ggttccgatc atggctgggg cagtcaccac ttcgtgatgg
     1621 gcggcgccgt gaacggcgga cgtttctacg gcaccgcgcc gcacgtctcg attcagaccc
     1681 ccgaccaggt cggacagggc cgactgttgc cgacgacctc ggtggatgaa ttcgccgcga
     1741 cgctcgcgcg ctggttcggt tgcaacacgg gcgagttgcc cgggatcctg ccgaacgtcg
     1801 ccaacttcac caacaccaac ctcggcttcg tttaatgggg acactcccca tttcctaaca
     1861 aaaggggccg gacctgttca gcgctccggc cacttccacg gtggtgcgtc gagcatgccc
     1921 tggccttcga tcgccgtctc accgagttcc ttggtcaacg tgatcgcatt gcagttcctg
     1981 gcttccttga gcgccgcgat catgcgcgag gcgtgcgaga cgacccacac ctgcgtccgc
     2041 gtcgacgcct tctcgatgag tcgcgcgagc gccggcagca gatccggatg caagctggtc
     2101 tcgggttcgt tgagaatcat gagcgacggc ggtcgcggcg tgagcagggc ggcaatccag
     2161 agtagatagc gcagggtgcc gtcggacagc tcggcggcgg tcagtggccg cagcagccca
     2221 tgctgtgtga agagcacggt gaactggccg ccgccgtgtt cgatgtcgac gcgagcgccc
     2281 gggaaagcgt cttcgaccgc ggcatcgagc gcgggccggt cgccgatctc gcgaatggtc
     2341 tgcaacgacg cggcgagatc gcggccgtcg tgcgccagaa caggcgtgcg tgtaccgatc
     2401 tgcgtatgcc gtgcgggcgc gtcggagtcg gcgcgaaagt gatcgtagaa gcgccaggac
     2461 cggacctctt cgcgcaacgc cagcagctcc ggcgcgcggt ccggatcggc aatcaccgag
     2521 agcatgcttt cgaaggttgg cagccgtgcg actgattcgt cccattcgcc atccgcgcgt
     2581 cgcagccgca cggcgggtcc gcggcggtcg gctaacaagg acgaaggccg cagcgcgggg
     2641 gcgccccaga cgcattcccg tttgatttcc gggtcctgcg tgaatgcact caaagaggga
     2701 atcggcaggc cgaggtcgat cgcgtatccc acgtgctcgc cctgaaatcc caggcgaagg
     2761 tgcacgggct ccttgcgtcg cccgccctgc accgcgaccg ttccttgttt cattgcgcgc
     2821 gaaatcgtct cgggcccggc ccagagcgtg gacgacaatc cgccttcgcg ggcgagcgag
     2881 ctcacgatgc cgccttgcgc cgcttcggcc agaagtcgca acgcgcggta cagactggat
     2941 ttgccgctgc cgttggcgcc tgtgatgaga tttagctggc cgagcggcat caccagcgcg
     3001 cggatggatc gataattcgc gacggcgagg gtttgcagcg ctggcatgac gccaagcgta
     3061 cccgcacccg ccgccgcaca cgagtacccg cgtgaggggt agttagcaaa ggcggggccg
     3121 ggcgcgtcga gggcgtcccg gcccgcgtca ggtgcggccc cttttgctag gaaatgggga
     3181 gtgtccccat tcagtcgatc ttgttgaagt tgatcgagcg gtccttgaag cggacgttcg
     3241 cccacttcgc ggcgtcttcg gacaacgcca gcaactgctc gatctccatg agcgcggcgt
     3301 tgtgatacgc ggcgagcagc tggcgattcg tggagctctg cgccgcggct ccatactccg
     3361 ccgggccgag gtcgcgctgg gcggcggcga tgtcggcgag gcgcgatttg cgggcggcct
     3421 ccacctgctg ggcaatggcg ggcagcgcct tgtcgtattc ggcgatgatc tgattgaacg
     3481 ccgcacgcag cgtggccttg tccgccgcct ggtcggcagc ggcgcatgag ccttcgccgt
     3541 cgctgcactt gagcgccttc accgccttgt ccgagatctg catcgcgcgc gtgttgacgt
     3601 cctgctggat ctttccgacc gcggtgtact gcgcggtgat accgccgctg gtcatctgct
     3661 tcgtctgata gtcagcatag tggtcggcgg ccgtctgcac ctcgtccgga tccgccgcca
     3721 tggcctgcac atccttgagc gcgttctggc tctgcgcctg cgccatctgc atgccgagct
     3781 tcatctgctc ttcctggctc atggccgcga tcttcgcctt cacctgatcg gcgtacgccg
     3841 ggtcacgctg ggcacgctgg aagtcgatgc cgccaccggt ctgggcggcc gtcggctgcg
     3901 cggcggcgcc ggcttcccgg ctgagtttct cgatgaaggc gcgttgatcc ttgacctgtt
     3961 tccgcaaggt ggcgagctcc gggctcgcaa gccaggcggg cgccgcggcg gcggtggccg
     4021 ggggcgcggg tacgcgcgtg tacagggact ggagcgattg ctgggccgag gcggcggtcg
     4081 cgaacaacag gctggccgcg agggcgacga acttgaacat gaacagaact ccggggattc
     4141 aaggggtttc gaagggcgcg gagtatgggc aggcgccctg gcctgcggaa gtgacaggtg
     4201 tcaacgcagt tatgaaaaat gagactttag ttgtggtcgg gtttgtaccc gcctgccttc
     4261 ggctattctc cacggcttcc tgagggggaa aaaacatgtc gctgactcgc ctgaatccgg
     4321 ttcgtttcgt aatgactttc gcgctggcca tgttgtttgg ggccgccaac gcttccgagc
     4381 agccgccgac gctggcgatc ggcgccgcca tgccgccgtt ctcgctgccg ggcatcgacg
     4441 gcaagaccta caccgacaag agtttcgcca aggccgacat cctggtgatg gtgttcacct
     4501 gcccgcattg ccccacggcg caggcctatc aggaacgcat caagaagctg gtgaccgatt
     4561 acgcgcccag gaacgtcacg ctgatcgcca tcaacccgaa ccacgcggat gccgtgcggc
     4621 tcgatgagct ggcctacagc gatctctccg attcgttcga ggaaatgcag gagcgcgccc
     4681 gcgaccagaa attcaatttc atctggctcg acgacggtcc caagcaggag atgtcgcatc
     4741 tctacggccc cgtggccacg ccgcatgtgt tcatcttcga caaggcgcgc aagctgcgct
     4801 tcgaaggccg catcgacgac tccgaacgcg aagctggcgc caccacgcac gacacgcgca
     4861 aggcgctgga tgcgttgtta gccggcaagg agccgccgat caccacgacc aaggtgttcg
     4921 gctgttcggt caagtgggcc gacaaagccc cggattacgc caactaccgg aagaagtggg
     4981 ccgccgagcc ggtgacgctg gaaaaggctg acgccgcggt gctcaaggcg atccgcgaga
     5041 acaaggattc cgggaagatc cgcttcatca atgtgtgggc cacgtggtgc gggccgtgca
     5101 tcaccgagtt cgacgaactg gtcgagcaca acctgcggtt ccgcattcgc ggcttcgaga
     5161 tggtgactat cgccgccgag tatcccgacc aggaagcgca ggtgaaggat ttcctcgtca
     5221 agaaacacgc gtcgatgaaa aactacatct tcgccaccga caagaaggac gagctcagcg
     5281 aagcgctcga tccggcctgg aagggtccgc tgccgtacac catcatcgtc gacgagaagg
     5341 gcaacgtgat ctaccgcgag atgggctcga tcaacttcct caagatgcgc cgcgccatcg
     5401 tgcccgcgct caatcgcctg acaccctgga agggactttc gcccgtcaag ccgtaagaac
     5461 cggggtcgcc gttcgtcatc tgtctgaacc cggacaggag gcgaaagtgt gcgtatcccc
     5521 atccttgcaa gcgcggctgc cgtgagcggt gccgccgatg aagacatcgc cgcgctcgcg
     5581 cggcaatacg gccccatcgt ttttcgcgcc gcctggcgcg tgctcggcgg gcgggccgcc
     5641 gcggaggacg tgcagcagca ggtttttctg cggctcatcg agtcaccgcc gcgcaacgtc
     5701 gagtcatggc cggcgttcct gacgacggcg tcaacccggc tcgccatcga tcatctgcgg
     5761 cgccagcggc gctggaggtt gttggcgccg ctctggaaag ccgacgagcc cgacgactca
     5821 ccggccgagg acgccgaacg cgctgaagcc ggccggttca tgcgcgaggc gctggcccgg
     5881 ctcaatccgc gtgacgcatc gtgtttcgtg atgcgccacc tgcacggcct cgcgcccgcc
     5941 gcgatcggtt ccgcgctggg catatccgaa aaccacgtca gtgtcagtgt gcatcgagca
     6001 agaaaagcgc tcgaagcgtt tcaggacaag gagcaatccc gatgaactct ctcgattcga
     6061 cccttggatt gtttgccgcg agtgaacctg acgcgcaggt ggtgagtgac gcgcagcgaa
     6121 aactcgagac ggtggtgagc aagcgcatgg cggagatttc ggcgcgccgt ccggcgcgta
     6181 ccgcgcgtgg ctggcttgcg gcgggcgcaa cagccgcggt cgtggcggtt gccatgttgt
     6241 ggttgcctct cgggaccacg ccggcgctgg cctttgcgaa ggtccaggag cacttccgcg
     6301 acttccgcac gctgcgcttc gttgtcgaac agcgcatgaa tggcgaggtt gtcatgaagg
     6361 cgcgcgtaag catggtccgc gatggaagcg tgcgca
//
LOCUS       DATDEU010000590         2987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1283969, whole genome shotgun sequence.
ACCESSION   DATDEU010000590 DATDEU010000000
VERSION     DATDEU010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283969"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VIV63_14160"
     CDS             <1..789
                     /locus_tag="VIV63_14160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6125792.1"
                     /translation="VIGLHPVIRSAVCDTISIECSHVISVDSPINAIEALQDFNSRIT
                     RVRIVVDANAAAKLPHAVSGLRSAATPRVLDVVALMPPDADATAPTGINRVLVKPLCT
                     TDLVAAPVVDTAQSTRIRAMPQSGSRGRALVVEDNAVNQEMARAMLDMLGFIVSTASN
                     GREGVLAAAADPELDLILMDCQMPVMDGLAAARAIREAEGGKSRVPIVALTGNAMPGD
                     REACVAAGMDDYLAKPFSLTALRTMIDRWTGSNTVAEAKALRKS"
     gene            complement(811..2124)
                     /locus_tag="VIV63_14165"
     CDS             complement(811..2124)
                     /locus_tag="VIV63_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HEY6125793.1"
                     /translation="MFTTAGAAEPPANKPVINVDAKIEQTGFVSVQGRHFKLGNKPYY
                     FAGANLWYGMYLGSPGKTGNRTRLIKELDQLAAQGITNLRVLAISESSTLKRSVTPAV
                     ILRPNHLDETLWQGLDFLLAEMAKRDMKAVLYLNNFWQWSGGMSQYVAWFNGKPVVDP
                     DVTGNWNEFMDNSAAFYREPKAQDAFRFAVKQLVTRKNSVNGRAYNADPTIMSWQLAN
                     EPRPGSDANARPHFDVFVKWIDDTAGFIKALAPKQLVSTGNEGWMGTAGSKELFVKSH
                     ATRNVDYLTFHMWAPNWTWFDPKKAEETYEGAWVKMQDYLNWHIDTANTMAKPIVLEE
                     FGINRDGGSFRPVATTVYRDKFYTAIFGLLARRAAAGDAIAGSNFWAWGGVGRTQNAD
                     FMWKAGDGFVGDPPQEPQGLYCVFDSDVSTINIISAHARQMSGMK"
     gene            2266..>2987
                     /locus_tag="VIV63_14170"
     CDS             2266..>2987
                     /locus_tag="VIV63_14170"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6125794.1"
                     /translation="MIRKLTTAAAAALLLPLIVSASIPPEEEVLEEIVTTGFRVSQGG
                     AQDIKFFRGEAEFQRIPHPNDFTAEGLMSEHDLVLPAAEACKQLFCLTGDATRADLLA
                     APQARYLVGVGFTTNIDEKKWKRNPVNLIAVVDKSGSMNGKPLELVRESLAEVAKQLR
                     TGDQMTIVLYGDTSHVHLGTTAVDRDGVDRILASISSIRSSGSTSMEAGLRLGYHIAD
                     LTAPAFKGRTRLLLFTDERPNT"
BASE COUNT          533 a          950 c          928 g          576 t
ORIGIN      
        1 gtcatcggcc tgcatccggt cattcgcagc gccgtctgcg acacgatatc gatcgagtgc
       61 tcacacgtga tcagcgtcga ttcgcccatc aacgccattg aggcgctgca ggatttcaac
      121 tcgcgtatca cgcgcgtgcg catcgtcgtc gatgccaacg ccgccgcgaa gcttccgcac
      181 gcggtcagcg gactgcgctc ggcggccacg ccacgtgtgc tcgacgtcgt ggcgttgatg
      241 ccgcccgatg ccgatgcgac tgcgccgacc ggcatcaatc gcgtgctggt gaaacccctg
      301 tgcacgaccg atctcgtcgc ggcgcccgtg gtcgacaccg cgcagagcac gcgcattcgc
      361 gcgatgcccc agtccggctc ccgcgggcgc gcgctcgtgg tggaagacaa cgcggtcaac
      421 caggagatgg cgcgagcgat gctcgacatg ctcggcttca tcgtctccac cgcgtcgaac
      481 ggccgcgaag gcgtactggc cgcggcggcc gatcccgaac tcgacctgat tctcatggac
      541 tgccagatgc ccgtcatgga tggccttgcg gccgcgcgcg cgattcgcga agcggaaggc
      601 ggcaagtctc gcgtgcccat cgtcgcgctg actggaaacg cgatgcccgg cgatcgggag
      661 gcctgcgtgg ccgcgggcat ggacgactat ctcgcgaagc cgttctcgtt gacggcgctg
      721 cggaccatga tcgatcgctg gaccgggtcg aacaccgtgg ccgaagcgaa ggcgctacga
      781 aagagctgag gacgccgcgt cagctccgtg tcatttcatg ccgctcatct ggcgcgcatg
      841 cgccgagatg atgttgatgg tcgagacatc cgagtcgaac acgcagtaaa ggccctgtgg
      901 ttcctgcggc ggatcgccga cgaagccatc tcccgccttc cacatgaaat ccgcattctg
      961 cgtgcgtccg actccgcccc acgcccagaa gttggagccc gcgatggcgt cgccggccgc
     1021 ggcgcggcgc gcgagcaggc cgaagattgc cgtgtagaac ttgtcgcgat agaccgtggt
     1081 cgcgaccgga cggaatgacc cgccgtcgcg attgatgccg aattcctcga gcacgatggg
     1141 cttggccatg gtgttggcgg tatcgatgtg ccagttcagg tagtcctgca tcttcaccca
     1201 cgcgccctcg taggtttcct cggccttctt cggatcgaac caggtccagt tcggcgccca
     1261 catgtggaag gtcaggtagt ccacgttccg ggtcgcgtgc gatttcacga acagttcttt
     1321 gcttcccgcc gtgcccatcc agccttcgtt gccggtgctc acgagttgtt tgggggccag
     1381 cgccttgatg aagcccgcgg tgtcatcgat ccacttcacg aacacatcga aatgcggacg
     1441 tgcattggca tcgctgcccg ggcgtggctc gttggcgagc tgccaggaca tgatggtcgg
     1501 atccgcgttg taggcgcggc cgttcactga gttcttgcgc gtgacgagtt gcttgacggc
     1561 gaagcggaac gcgtcctgcg ccttcggttc gcggtagaac gcggccgagt tgtccatgaa
     1621 ttcgttccag ttgccggtga cgtccggatc gaccacgggc ttgccgttga accacgccac
     1681 gtactgcgac atgccgcccg accactgcca gaagttgttg aggtagagca ccgctttcat
     1741 gtcgcgcttc gccatctcgg cgagcaggaa gtcgaggccc tgccacaagg tttcatcgag
     1801 gtggttcggc cgaaggatga ccgccggggt caccgaacgc ttgagcgtgc tggattcgga
     1861 tatggcgagc acacgcagat tggtgatgcc ctgcgccgcg agctggtcca gctccttgat
     1921 cagccgcgtg cgattgccgg tcttgccggg agatccgagg tacatgccgt accagagatt
     1981 ggcgcccgcg aagtagtaag gcttgttgcc gagtttgaag tgtcggcctt ggaccgagac
     2041 gaaacccgtt tgttcaatct tggcgtcaac attgattacc ggcttgtttg ccggcggctc
     2101 cgcagcgccc gccgtcgtga acattgtgag ggcaatagtt aacgctaacg cgagcaagtt
     2161 acggtttttc ataggagcgc gagtctaacc tttcgaaaag cgagcgtgaa ctttcgtttt
     2221 tcgcggagta cgctcgattg caagaacaac aatgggagga ttgccatgat tcgcaaactc
     2281 accacggccg ctgccgccgc gctgctcctg cccctgattg ttagcgcctc cattccgccg
     2341 gaggaagaag tcctcgagga aatcgtcacc accggctttc gcgtgagcca gggcggcgcg
     2401 caggacatca agttcttccg cggcgaagcc gaattccagc gtattccgca tcccaacgat
     2461 ttcaccgccg aagggctgat gagcgagcac gatctcgtgt tgccggccgc ggaggcctgc
     2521 aagcagctct tttgccttac cggcgacgcg acacgcgcgg atctgctggc cgcaccgcag
     2581 gcgcgttatc tcgtcggcgt cggcttcacc accaacatcg acgaaaagaa atggaagcgc
     2641 aatccggtga acctcatcgc cgtcgtcgac aagtccggtt ccatgaacgg caagccactc
     2701 gaactcgtgc gtgaaagcct cgccgaagtc gcgaagcaac tgcggaccgg cgaccagatg
     2761 accatcgtgc tgtatggcga cacctctcat gtgcatctcg gcaccactgc cgtcgatcgc
     2821 gatggcgtcg accggatcct cgcgtccatc tcgtccatcc gcagttcggg atcgacgtcg
     2881 atggaggcag gcctgcggct gggctaccac atcgccgacc tcaccgcccc ggcattcaaa
     2941 ggccgtaccc ggctgctgct gttcaccgat gagcggccga ataccga
//
LOCUS       DATDEU010000591         9406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1575843, whole genome shotgun sequence.
ACCESSION   DATDEU010000591 DATDEU010000000
VERSION     DATDEU010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575843"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VIV63_14175"
     CDS             complement(<1..126)
                     /locus_tag="VIV63_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006792396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY6125795.1"
                     /translation="MRGGGNMMKQAMALQANMKKAQDEIANMEVIGESGGGMVKVT"
     gene            complement(142..1734)
                     /gene="dnaX"
                     /locus_tag="VIV63_14180"
     CDS             complement(142..1734)
                     /gene="dnaX"
                     /locus_tag="VIV63_14180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY6125796.1"
                     /translation="MSYLALARKWRPRSFAELVGQEHVRMALGNALTQGRVHHAFLFT
                     GTRGVGKTTIARILSKCLNCDTGVTATPCGTCASCKEIDEGRFVDLIEVDAASRTKVD
                     DTRELLENVQYAPTRGRYKVYLVDEVHMLSTHSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPVTVLSRCLQFNLKRLPVVLISEHLSKVLTAEKIPFDAPALRLVAHAADGSMRDAL
                     SLLDQLIAFGGGKVEEAGARAMLGTIDRDHVTKLARSLATGDAAQLLETARSLEEFSP
                     DYGQVLDDLAALLTRLALNQLVPGYDGDELFDVALVKELSAAIPAEDVQLFYQTAILG
                     RRDLPFAPDPRTGFEMTLIRMLAFRPAGGAAVATTTGGGSTRPASAATGSNNALPSTV
                     TAAIVRPASAGPINPVQWSRVIGDLDVVGAARELATNVALIEHSGNLLRFAIDPKIVP
                     TATQVERLSQALAKYLGTRVKLEFVADKAAPVETPAQTGERRSAEALETARRTLEDDA
                     TVREMKSRFGATLHPESVRPTE"
     gene            complement(1767..1863)
                     /gene="ffs"
                     /locus_tag="VIV63_14185"
     ncRNA           complement(1767..1863)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIV63_14185"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1885..1974)
                     /locus_tag="VIV63_14190"
     tRNA            complement(1885..1974)
                     /locus_tag="VIV63_14190"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Ser,seq:gga)
     gene            complement(2034..2321)
                     /locus_tag="VIV63_14195"
     CDS             complement(2034..2321)
                     /locus_tag="VIV63_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125797.1"
                     /translation="MRIPALLIASVVALVLLPGCKTRGPTCKQDNKDYVGAQELPPLK
                     APPGLEPPDTRNALKVPPLDSPERPRGKNEACLDVPPPFANPKGPDAAKPR"
     gene            complement(2345..3256)
                     /gene="dapA"
                     /locus_tag="VIV63_14200"
     CDS             complement(2345..3256)
                     /gene="dapA"
                     /locus_tag="VIV63_14200"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY6125798.1"
                     /translation="MFAGSLVALVTPMQPDGSIDFDAWSRLLEFHVANGTVGIVVAGS
                     TGESATVTDAELRELLVHARRVLGKRALLVANAGTNGTATSVERARWISEIAVDALLM
                     ATPGYVRPTQQGLFLHFEAIAANSRIPVLLYNVPGRTAVDLLPATVARLSRLPRIAGI
                     KEAVTDPARIRELVASCAAEFKVLSGDDLTARDSIGLGAAGVISVTANVAPRAMADMV
                     AAAVRGDRAGATQIDQRLVALHRNLFVETNPIPVKWAVARMGLIGGTLRLPLTELSPE
                     FHGVIAESLFAAGITVDAGQQMPAVNS"
     gene            3350..3880
                     /locus_tag="VIV63_14205"
     CDS             3350..3880
                     /locus_tag="VIV63_14205"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY6125799.1"
                     /translation="MKQHLAVSAIGSDRTGMVHDLTRVISDCGGSISESRMASLGNEF
                     AMLVLVSGNWHSLAKLESELARLGEASGLSLHMKRTEPRAPRTELLPYSIDVVCLDQA
                     GIVTGLSGFCASRGIDIAEVSTRTYPAAHTGAQMFAVQMIINVPKRLQLAHLREEFMD
                     YCDAQNLDAILEPVKS"
     gene            3877..4353
                     /locus_tag="VIV63_14210"
     CDS             3877..4353
                     /locus_tag="VIV63_14210"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY6125800.1"
                     /translation="MTVAVGKKVPAFSLPVSDGSVWKSADSFSGKNSGKLVLYFYPKD
                     MTPGCTLEGQDFRDLHAAFKRAKTTVLGVSKDSCERHAKFRTREKFPFELLSDEDEKV
                     CRLFDVIREKSLYGRKFMGIERSTFLIDESGKLVREWRKVRVKGHAAEVLEAAKSL"
     gene            4350..5807
                     /locus_tag="VIV63_14215"
     CDS             4350..5807
                     /locus_tag="VIV63_14215"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY6125801.1"
                     /translation="MTSKRAAGGKPAIVNERRLFVLDTNVLMHDPTAIFRFQEHDVYL
                     PMVVLEELDSNKKGLSEASRNVRQTSRFLDDLMRDATKEQIDAGLPLPSAMNGPGSGA
                     SGKAGAGKAGRLFFQTRQLTSALPDYLPGHGADNAILAQTLALQALHHEARVTLVSKD
                     INLRIKAAVVGVHAEDYYSDKTIEDADVLYTGLAALPADFWEKAGKDIKSWKEGGRTF
                     YQVTGPMVADLHVNQFVHEAGESGIEAIVRRIEAGTATLELARDFRGERHSVWGVNAR
                     NREQNFALNLLMDPEIDFVTILGPAGTGKTLLTLAAGLMQTLESNRFVEIIMTRVTIP
                     LGEDIGFLPGTEEEKMEPWMGALMDNLEVLTKTGEGGNWGRAATNDLLRNRIKIRSLN
                     FMRGRTFLNRFVILDEAQNLTPKQMKALVTRAGPGTKIVCLGNIAQIDTPYLTETTSG
                     LTYVVNRFRGWGHSGHITLVRGERSRLADFASDTL"
     gene            5845..7395
                     /locus_tag="VIV63_14220"
     CDS             5845..7395
                     /locus_tag="VIV63_14220"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY6125802.1"
                     /translation="MPSGPKKLLVALLSVLLPLQSFAQSATANDLPDIGSPASSSLSL
                     DDEYHIGLQVMRQLRDEGQILEDPEATEYLQALGSRIVAQATGDSAQRFSFFFVNDDT
                     INAFALPGGFIGVNHGLVLATRNEAQLAGVLAHEIAHVTQRHIARRVRSQGRQSIATM
                     AAILAAILVGAATGSSDAALGGISVAQGAAMQQQINFTRANENEADRVGMSFLASAGF
                     DPYGMPDFFETMGRRNTVLATNRNALPEILQSHPVTTNRIAESRARAAQFKELKQIAE
                     SVSYSLTRERLRVLATPAEDNVRRYYMERREQQEQTVGERYGEALASYQGGSSRASLD
                     ALTELARKYPQVPMLQSTLGQALMASGETEEALATFRRALNLSPRNIPLTMRYAEALM
                     KADQPKAAHAVLLDLFNNVAPTPEQIRFTALAASSAGDTGDAAYYMSEYHIATGNLPL
                     SVAQLEMALAAPNITAVQRSRYQARLDEVREVLFSDRKRRKNNEQQKPPEQQSQRPVA
                     NRAGTISR"
     gene            7392..8186
                     /locus_tag="VIV63_14225"
     CDS             7392..8186
                     /locus_tag="VIV63_14225"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY6125803.1"
                     /translation="MKNWLLLIAGLMLTGCATVPSGKTDPRDPWERFNRTTFKFNDAL
                     DRAIAKPVAKGYVKVTPRVVRTGISNFFNNLDTVPTIVNDALQGKFRQAGHDSARFLL
                     NSTLGLGGLFDPASAAGLDANDEDLGQTFGKWGVKPGPYLVLPVLGPSTTRDAFSRLA
                     DTYLEPVWYLEDDSTRYLIRVIDLLDQRASLLELESQLARSYDRYAFIRNAWLQRREY
                     QVKDGNIEDDSLELEQEFMDQPPEGTSAEPPAESPPPAESSTPPPQ"
     gene            8214..8543
                     /locus_tag="VIV63_14230"
     CDS             8214..8543
                     /locus_tag="VIV63_14230"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY6125804.1"
                     /translation="MSTPIDPGKLPPLLLLHPDDNILVARRDIAAGETVEMDGETFDM
                     PVAIELGHKLARRALAPDTRVLKYGAPIGSMKMAVARGEHVHLHNLRSDYLPSTSREI
                     DKKTDGH"
     gene            8543..>9406
                     /locus_tag="VIV63_14235"
     CDS             8543..>9406
                     /locus_tag="VIV63_14235"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY6125805.1"
                     /translation="MNAVAMTSPSMQGYLRKDGRKGIRNVVAVAYLVECAHHVSRLIV
                     QKSGSDDVHLIGFPGCYPNKYSLEMMQRLCTHPNVGAVLLVSLGCEGFNREQLRRTIA
                     ASGRPVDLLVIQQSGGTTRTLNAGLDIVRKMRAELAKQIPVPMAFEELIVGTVCGGSD
                     GTSGITANPAVGRCFDWLIEKGSACIFEETGELIGCERIMASRAITPALGVEIEACVD
                     KAANYYRIMGYGSFAPGNAEGGLTTLEEKSMGAYSKSGSSPISGLIKPGDVPPRGGLY
                     LLDVVPDGEPRF"
BASE COUNT         1795 a         3123 c         2924 g         1564 t
ORIGIN      
        1 ggtcaccttg accatgccgc caccggattc accgatgact tccatgttgg cgatttcgtc
       61 ctgggccttc ttcatgttgg cctgcagtgc catcgcctgc ttcatcatgt ttccaccacc
      121 acgcatcgat atttcctcca tctactcggt tggccgcacc gattccggat gcagcgtggc
      181 gccgaaacgc gatttcattt cgcgcaccgt tgcgtcgtct tccagtgtgc ggcgcgccgt
      241 ctcgagcgct tccgcggaac ggcgttcgcc ggtttgcgcc ggtgtctcga ccggcgcggc
      301 cttgtcggcg acgaactcga gtttcactcg agtgccgagg tacttcgcga gcgcctgcga
      361 caaccgctcg acctgggtcg ccgtcggcac gattttcgga tcgatcgcga acctcagaag
      421 gttgccactg tgttcgatca gcgcaacgtt cgtggcgagt tcgcgcgcgg caccgaccac
      481 gtcgagatcg ccgatgactc gcgaccattg caccgggttg atgggacccg cggaagccgg
      541 acgaacgatg gccgcggtca cggtcgacgg cagtgcgttg ttcgaaccgg tcgccgccga
      601 tgccgggcgc gtggagccac cgccggtcgt cgtggccaca gccgcaccgc cggcagggcg
      661 gaacgccagc atgcgtatca gcgtcatttc gaaaccggtg cgcgggtcgg gcgcgaacgg
      721 cagatcgcgc cggccgagga tggcggtctg gtagaagagc tgcacgtctt ccgcggggat
      781 cgcggcggac aattccttca ccagcgccac gtcgaacaac tcgtctccgt cgtatcccgg
      841 cacgagttga ttcaatgcaa gacgcgtcag cagcgcggcg agatcgtcga gaacctggcc
      901 gtagtcgggc gagaattctt cgagcgagcg cgcggtttcg agcagctggg cggcatcacc
      961 cgtggcgagc gagcgcgcga gtttcgtgac gtggtcgcga tcgatcgtgc cgagcatggc
     1021 tcgcgcaccg gcctcctcga ccttgccgcc gccgaacgcg atgagctgat cgagcaacga
     1081 caacgcgtca cgcatgctgc cgtcggcggc atgcgccacc agccgcaggg ccggcgcgtc
     1141 gaacgggatt ttctcggccg tcagcacttt gctcagatgc tcgctgatga gcacgaccgg
     1201 caggcgcttg aggttgaact gcaggcagcg cgacaacacg gtgaccggca acttctgcgg
     1261 atcggtggtg gcgagcagga atttgacgtg cggcggaggc tcttcgagcg tcttgagcag
     1321 cgcattgaac gaatgcgtcg acaacatgtg cacttcgtcg acgagataga ccttgtagcg
     1381 gccgcgcgtg ggcgcgtact gcacgttctc gagcaactcg cgcgtgtcat cgaccttggt
     1441 gcgcgacgcg gcgtccactt cgatgaggtc gacgaaacgg ccctcgtcga tttccttgca
     1501 cgaagcgcag gtgccgcacg gcgtggcggt gacacccgtg tcgcagttga gacacttgga
     1561 gaggatgcgc gcgatggtgg tcttgcccac gccgcgcgtg ccggtgaaca ggaaggcgtg
     1621 atgcacgcgc ccttgcgtga gcgcattgcc caacgccatc cgcacgtgtt cctgcccaac
     1681 gagctccgcg aaggaacgag gtcgccactt acgcgccagc gcgagataac tcatcgtttc
     1741 ggcaatcctt gttggaggga ggcagcccgc accagccgga cttcggcgcc cgacatcgcc
     1801 gctaccgctg ctcccttccg ggcctgacgg ggtttacaac tagtcgtcgc gaaggagccg
     1861 atacggactg ccatggtaaa gaagtggcgg acagacaggg attcgaaccc tggaacaccg
     1921 tgagatgtta cttgctttcc aagcaagcgc cttcgaccgc tcggccatct gtccgttacc
     1981 cgcgcaaacc cttcgtgccc agggccttca aggggggcga atgataccaa tagctaacgg
     2041 ggcttggcag cgtccgggcc cttcggattg gcaaaaggag gcggcacgtc gaggcaggct
     2101 tcgtttttcc cgcggggacg ctcgggggag tccagcggcg ggaccttgag ggcattacgc
     2161 gtatcgggcg gctccaggcc gggaggcgcc ttgagcggcg gcagttcctg ggcgccgacg
     2221 tagtccttgt tgtcctgctt gcaggtgggg ccacgagtct tgcagcccgg caacagaacc
     2281 agggccacga cacttgcgat caggagagcg ggtatacgca tgaggggtgc gaccttatcc
     2341 gacttcagga gttcacggcc ggcatttgtt ggccggcgtc gacagtgatt ccggccgcga
     2401 acagcgactc cgcgatcacg ccatggaatt ccggggatag ctcggtcaac ggcaggcgca
     2461 gcgttccgcc gatgagtccc atccgggcca ccgcccactt cacgggaatg ggattggtct
     2521 cgacgaaaag attgcgatgc aaagccacga gacgctgatc gatctgcgtt gcgccagcgc
     2581 ggtcgccccg cacggccgcg gccaccatgt ccgccatggc gcgcggcgcc acattggccg
     2641 tcacggagat gacgcctgcg gcgccaaggc caatggagtc acgcgcggtg agatcgtcgc
     2701 cactcagaac cttgaactcc gcggcgcagc tggcgaccag ttcgcggata cgcgcgggat
     2761 cggtgaccgc ttccttgatg ccggcaatac gcggcaatcg ggacagtctc gccacggtgg
     2821 ccggcaacag gtcgaccgcc gtgcgccccg gcacgttgta cagcaacacg ggaatgcgag
     2881 aattcgcggc gatcgcttcg aagtgcagga agagtccctg ctgcgtcggc cgcacgtagc
     2941 cgggtgtcgc catgagcagc gcatcgacgg caatttcgga aatccaccgt gcgcgttcga
     3001 ccgacgtcgc ggtgccattg gtgcctgcgt tggcgacgag caacgcgcgt ttgccgagca
     3061 cgcggcgcgc atggacgagc aactcgcgga gttcggcgtc ggtgacggtg gcggattcgc
     3121 ccgtggagcc ggcaacgacg attcccaccg tgccattggc gacatgaaat tcgaggagac
     3181 gtgaccaggc gtcgaaatcg atactgccgt ccggctgcat cggggtcacg agcgccacca
     3241 ggctgccggc gaacatgcgt tagatccgag gtcgtttaaa cgggcgggaa tggtacgggc
     3301 gcacacccgc cattcgcaag ccaaaaggct gcggtacact cgccgccaga tgaagcaaca
     3361 cctcgccgta tctgccattg gcagcgatcg caccgggatg gtccacgacc tgacacgcgt
     3421 gatttcggac tgcggtggat ccatcagcga gagccgtatg gcgagcctcg gcaacgagtt
     3481 cgccatgctc gtgctggtca gcggcaactg gcactcgctc gccaagctcg aaagcgagct
     3541 ggcccgcctg ggtgaagcca gcggcctgtc cctgcacatg aagcgcacgg aaccgcgcgc
     3601 gccgcgcacc gaactattgc catattccat cgacgtggtg tgcctcgacc aggccggcat
     3661 cgtcaccgga ctttccgggt tctgcgcgag ccggggcatc gacatcgccg aagtctccac
     3721 ccgcacctat cccgcggcgc acactggcgc gcagatgttc gcggtgcaga tgatcatcaa
     3781 cgttccgaag cgcctgcagc tcgcgcacct gcgcgaagaa ttcatggatt actgcgatgc
     3841 gcaaaacctc gatgcgatcc tggagcccgt caagtcttga ctgttgcggt tggcaagaaa
     3901 gtgcccgcgt tctcgctgcc ggtctctgat ggcagcgtgt ggaaatccgc tgattccttt
     3961 tcgggaaaaa acagcggcaa actggtgctc tacttctacc ccaaggacat gacgccgggc
     4021 tgcacgctcg agggccagga tttccgcgac ctgcatgcgg ccttcaaacg cgcgaagacc
     4081 acggtgctcg gcgtgtccaa ggacagctgc gaacgtcacg cgaaattccg taccagggaa
     4141 aaattcccgt tcgaactgct ttcggacgag gatgagaaag tttgccgact cttcgatgtg
     4201 atccgcgaga aatcacttta cggaaggaag ttcatgggaa tcgaacgcag cacgttcctg
     4261 atcgacgaaa gcggcaagct ggtccgcgaa tggcgcaagg tgcgcgtcaa gggccacgcg
     4321 gccgaagtgt tagaggcggc caagtcactg tgacatcgaa acgcgcggcc ggcggcaagc
     4381 cggcaatcgt caacgaacgc cgcctgttcg tgctcgacac caacgtgctc atgcacgacc
     4441 ccaccgcgat attccggttc caggaacacg acgtgtacct gccgatggtg gtgctcgaag
     4501 aactcgactc caacaagaaa ggtttgtcgg aagcgtcgcg caacgtgcgc cagacgagcc
     4561 gcttcctcga cgacctgatg cgcgacgcca cgaaggaaca gatcgatgcc ggtctgcccc
     4621 tgccctcggc catgaacgga ccgggcagcg gcgcctcggg caaagccgga gccggcaagg
     4681 ccggccgcct cttcttccag acgcgccagc tcacctcggc gctgccggac tatctaccgg
     4741 gtcacggcgc cgacaacgcc atcctcgcgc agacgctcgc gctgcaggcg ctgcaccacg
     4801 aagcacgcgt gacgctggtc tccaaggaca tcaacctgcg catcaaggcc gccgtggtcg
     4861 gtgtgcacgc cgaggactac tacagcgaca agaccatcga agacgccgat gtcctgtaca
     4921 cgggactcgc ggcgctgccg gcggatttct gggagaaggc cggcaaggac atcaagtcct
     4981 ggaaggaagg cggccgcacg ttctaccagg tgaccggccc gatggtcgcc gacctgcacg
     5041 tcaaccagtt cgttcacgaa gccggcgagt ccgggatcga ggccatcgtc cgccgcatcg
     5101 aggccggcac cgccacactc gagctggcgc gcgacttccg cggcgaacgc cacagcgtgt
     5161 ggggcgtgaa cgcccgcaac cgcgagcaga atttcgcgct caacctgctc atggatccgg
     5221 agatcgattt cgtcaccatc ctgggaccgg cgggcaccgg caagacgctg ctgacgctcg
     5281 cggccggact catgcagacg ctggaatcga accgcttcgt cgagatcatc atgacgcgcg
     5341 tgacgatccc gctcggcgaa gacatcggct tcctgcccgg tacggaagaa gaaaagatgg
     5401 agccctggat gggcgccctc atggacaacc tggaagtgct gaccaagacg ggtgaaggcg
     5461 gcaattgggg acgcgcggcg accaacgacc tgctgcgcaa ccgcatcaag atccgctcgc
     5521 tcaatttcat gcgcggccgc acgttcctga accgcttcgt catcctcgat gaggcccaga
     5581 acctgacccc gaaacagatg aaagccctgg tcacccgggc aggccccggc accaagatcg
     5641 tttgcctggg caacatcgcg cagatcgaca ccccctacct caccgaaacc acgtccggtt
     5701 tgacttacgt cgtgaaccgc ttccggggct gggggcattc cgggcacatc acgctggtcc
     5761 gtggcgagcg ctcgcgtctg gccgatttcg cctctgatac cctctagtcg aacttaaagg
     5821 gcgcactcag ggtccaattt cgcgatgcca agcggtccga aaaaactcct cgtcgccctg
     5881 ttgtcggtgc tcctgccgct gcagtccttt gcgcagtcgg ccaccgccaa cgacctgccg
     5941 gacatcggca gccccgcgtc gtcctcgctg tcgctcgacg acgagtatca catcggcctg
     6001 caggtcatgc gccagctgcg cgacgaaggc cagatcctcg aagaccccga agccaccgaa
     6061 tatctgcagg cgctgggctc gcgcatcgtc gcgcaggcca cgggcgattc cgcgcagcgt
     6121 ttctcgttct tcttcgtcaa cgacgacacc atcaacgcct tcgcgttgcc gggcgggttc
     6181 atcggcgtca atcacggcct ggtgctcgcc acgcgcaacg aagcacagct cgccggcgta
     6241 ctggcgcacg aaatcgccca cgtcacgcag cgtcatatcg cgcgccgcgt gcgatcccag
     6301 ggacggcaga gcatcgcgac catggccgcc attctcgcgg ccatcctcgt gggcgccgcc
     6361 accggttcca gcgacgcggc actgggcggc atctccgtcg cacaaggcgc ggccatgcag
     6421 cagcagatca acttcacgcg cgcgaacgag aacgaggccg atcgcgtcgg catgagcttc
     6481 ctcgcctcgg ccggcttcga tccctacggc atgcccgact tcttcgaaac catgggccgg
     6541 cgcaataccg tactcgcgac caaccgcaat gcgctgccgg aaatcctgca atcgcacccg
     6601 gtcaccacca accgcatcgc cgaatcgcgc gcccgcgccg cgcagttcaa ggaactcaag
     6661 cagatcgccg aaagcgtgag ctactcgttg acgcgcgaac gcctgcgcgt gctcgccacg
     6721 cccgccgaag acaacgtgcg gcgctattac atggagcgcc gcgaacagca ggaacagacc
     6781 gtcggcgaac gatacggcga agcgctggct agctaccagg gcggcagctc acgcgcctcg
     6841 ctcgacgcgc tgaccgagct cgcgcgcaaa taccctcagg tgcccatgtt gcaatccacg
     6901 ctcgggcagg cgctcatggc gtcgggcgaa acggaagaag cattggccac gttccgccgc
     6961 gcgctcaatc tctcaccgcg caacattccg ctcaccatgc gttatgccga ggcgctgatg
     7021 aaagccgacc agccgaaagc cgcgcacgcg gtgctgctcg acctgttcaa caatgtcgcg
     7081 cccacgccgg agcagatccg cttcactgcg ctcgcggcga gcagcgccgg cgacacgggc
     7141 gacgccgcgt attacatgag cgaataccac atcgccaccg gcaacctgcc gttgtcggtg
     7201 gcgcaactcg aaatggcgct cgcggctccg aacatcaccg cggtccagcg cagccgctat
     7261 caggcgcgcc tcgatgaagt gcgtgaagtg ctgttctccg atcgaaaacg gcgcaagaac
     7321 aacgagcagc aaaagcctcc ggagcagcaa tcccagcgtc ccgtggcgaa tcgggctggt
     7381 acaatttcgc gatgaaaaat tggcttctgt taatcgctgg tctcatgttg accggctgcg
     7441 ctacggtgcc ctcgggcaaa accgatccac gcgatccctg ggagcgattc aaccgcacca
     7501 cgttcaagtt caatgacgcg ctcgaccgcg ccatcgccaa acccgtcgcc aagggttacg
     7561 tgaaggtcac gccgcgcgtg gtgcgcaccg gcatcagcaa cttcttcaac aacctcgata
     7621 ccgtgcccac catcgtcaac gatgcgctgc agggcaagtt ccgccaggcc ggccacgatt
     7681 cggcgcgatt cctgctcaac agcacgttgg gcctgggtgg cctgttcgat ccggccagcg
     7741 ccgccggcct cgatgccaac gatgaagacc tcggccagac cttcggcaaa tggggcgtga
     7801 agcctggtcc gtacctcgtg ttgcccgtgc tcgggccctc cacgacgcgc gatgctttct
     7861 cgcggctggc cgacacgtac ctcgagccgg tctggtatct cgaggacgac agcacgcgct
     7921 acctgattcg cgtcatcgac cttctggatc agcgcgcgag cctgctcgaa ctcgaaagtc
     7981 agctggcgcg cagctacgac cgttacgcgt tcatccgcaa cgcctggctg cagcggcgcg
     8041 aataccaggt gaaggacggc aatatcgaag acgattcgct cgagctggaa caggaattca
     8101 tggaccagcc gcccgaaggc acctcggcgg aaccgcccgc ggagtcgcct ccgcccgcgg
     8161 aatcctccac gccgcccccg caatagctag acttgcggca gacagggggc cgaatgagca
     8221 caccgatcga tccgggcaag ctgccgccac tgctgctgct gcaccccgac gacaacattc
     8281 tcgtcgcacg gcgcgatatc gcggccggtg aaaccgtgga aatggacggg gaaaccttcg
     8341 acatgcccgt ggcgatcgag cttggtcaca aactcgcccg ccgggccctt gcgcccgaca
     8401 cccgcgtcct caagtatggt gcccccatcg gctccatgaa gatggcggtg gcgcgcggag
     8461 aacacgtgca cctgcacaat ctgcgcagcg actatctccc ctcgacctcc cgggaaatcg
     8521 acaagaagac ggacggacac tgatgaacgc ggtggcgatg accagccctt cgatgcaggg
     8581 atacctgcgc aaagacggcc gcaaaggcat ccgcaacgtg gtcgcggtgg cctacctggt
     8641 ggaatgcgcg caccacgtct cgcgcctcat cgtgcagaaa tccggcagcg acgacgtcca
     8701 cctcatcggt tttccgggct gctaccccaa caagtattcg ctcgagatga tgcagcggct
     8761 gtgcacgcac ccgaacgtgg gcgccgtgct gctcgtgtcg ctgggctgcg aaggtttcaa
     8821 tcgcgagcaa cttcgccgga ccatcgccgc cagcggccgg ccggtggatc tgctggtcat
     8881 ccagcaatcc ggcggcacca cgcgcacgct caacgccggc ctcgacatcg tgcgcaagat
     8941 gcgcgccgag ctcgccaaac agattcccgt gccaatggcg ttcgaagaac tcatcgtcgg
     9001 caccgtgtgc ggcggttcgg acggcaccag cggcatcacc gccaatcccg cggtcggccg
     9061 ctgcttcgac tggctgatcg aaaaaggctc ggcctgcatc ttcgaggaaa ccggcgagct
     9121 catcggctgc gaacgcatca tggcgtcgcg cgccatcacg cctgcgctcg gcgtcgagat
     9181 cgaggcctgc gtcgacaaag ccgctaacta ctatcgcatc atgggttacg gcagcttcgc
     9241 gccgggcaat gccgagggcg gcctgaccac gctcgaagaa aagtccatgg gtgcgtattc
     9301 gaagtccgga tcgtcgccca tttcgggact catcaagccc ggcgacgtgc cgccgcgcgg
     9361 cgggttgtac ctgctcgacg tcgtgccgga tggcgaaccg cgcttc
//
LOCUS       DATDEU010000592         2922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_556871, whole genome shotgun sequence.
ACCESSION   DATDEU010000592 DATDEU010000000
VERSION     DATDEU010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556871"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VIV63_14240"
     CDS             complement(<1..1433)
                     /locus_tag="VIV63_14240"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY6125806.1"
                     /translation="MVVMGVTAITLAAAYAFVCSYVYLEPTLPSVSAMKNNELQVPLR
                     VYTNSGELIAQIGEQRRNPVKYDQIPLVVRQAFIAAEDDRFFEHHGIDWQGVLRSLFV
                     NLTSRETQGGSTITMQAARSAFFTQEQTVRRKLQEIFVTYRLEREFTKQEILALYLNV
                     IFFGQRSYGVASAAETYFGKPLDDLTLGEAATLARVPQWPSRYNPITNPAGAAERRGY
                     VLRRMRELGYIDATAADAASKEVVRAKAHGALADVEAPYVAEMVRLEILKRFGPTAQD
                     AGYKVYTTIDGRLQKAANRALRIGLVDYSRRHGWSGTTNRTELTGSETPDALETLLDE
                     YGTVGILQPAIVISVAEKQARVYVKGSGLTAIEWAGLSWAKRRVSELALGPEPKTAGE
                     VLSRGDVVYVVHEKADGPAELAQLPEAESALVALDPNNGAIISLVGGFDYFEGQGKYN
                     RVIQAKRQPGSGFKPFMYAAAMAGDFTP"
     regulatory      1516..1605
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1833..2900
                     /locus_tag="VIV63_14245"
     CDS             1833..2900
                     /locus_tag="VIV63_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY6125807.1"
                     /translation="MLFLTRKYRPLLGLDITTSSVKLIELVTSGGAYRVESYAAEPTP
                     QNAMNEKAIVDAEAVGEAIRRAVKRSGAGAKEVAIAISGDAAITKVIQMPRSLRERDL
                     EAQVEMQADQYIPFPMDEVSYDFEVLGPSEKDPETNDVLLVATRTENVEQRQAAVNAA
                     GLTAKIVDVEAFALENACRLMTHQMPDGGIDRTIAVVDFGASSTTFSVLRNLKVVYTR
                     DFAFGGQQLTEEIMRTYGLSMEEAGRAKKEGGLPGNFQAEVLDPFIDDMTQQVSRSLQ
                     FYLASGSGRDQPEKILICGGCANISGVSDVISSRVGISAEKGDPLGQMKLSSRAKAQA
                     VARDATALLTACGLALRSFDN"
BASE COUNT          563 a          906 c          884 g          569 t
ORIGIN      
        1 ggcgtgaaat cgccggccat ggccgcggca tacatgaacg gcttgaagcc ggagccaggt
       61 tgccgcttgg cctggatcac acggttgtac ttgccctgtc cctcgaagta atcgaagccg
      121 cccaccagcg aaatgatggc gccgttgttg gggtcgagcg ccacgagcgc gctttccgct
      181 tcaggaagtt gcgcaagttc agcgggacca tcggccttct catgcacgac gtagacaaca
      241 tcgccccgcg aaagcacttc gccagcggtc ttcggctcgg gcccgagcgc gagttccgaa
      301 acacgccgct tcgcccacga aagcccggcc cattcgatgg ccgtgagccc ggatcccttc
      361 acgtaaacgc gcgcctgctt ctccgcgacc gaaatgacga tggccggctg caggatgccg
      421 acggtgccgt actcatccag caaagtctcg agagcgtcgg gtgtttcgct gcccgtgagc
      481 tctgtcctgt tagtggtgcc gctccagccg tgccggcgcg aatagtcgac caggccgatg
      541 cgcagcgcgc gattcgccgc cttctgcaat cggccgtcga tggtggtgta caccttgtag
      601 cccgcatcct gcgccgtagg tccgaaacgt ttgaggatct cgaggcgcac catctcggcg
      661 acgtacggcg cctcaacgtc ggccagcgcg ccgtgcgctt tcgccctaac tacttccttc
      721 gatgccgcat cggcagcggt ggcgtcgatg taaccgagct cgcgcatgcg gcgcagcacg
      781 taaccgcgcc gttccgccgc acctgccgga ttggtgatcg ggttgtatcg cgacggccac
      841 tgcggcacgc gcgcgagcgt cgcagcttcg ccgagcgtga ggtcgtcgag cggttttccg
      901 aagtaggttt cagctgcgga agcgacgccg tacgagcgct gtccgaagaa gatgacgttc
      961 aggtagagcg cgagaatttc ctgcttggtg aattcgcgtt ccaggcgata tgtgacgaat
     1021 atttcctgca gcttgcggcg gaccgtctgc tcctgcgtga aaaacgcgct gcgcgccgcc
     1081 tgcatggtga tcgtgcttcc gccctgcgtc tcgcgagacg tcaggttgac gaacaagctg
     1141 cgaagcacgc cttgccagtc gatgccgtgg tgctcgaaga agcggtcgtc ttccgcggca
     1201 ataaacgcct ggcgcacgac gagagggatc tggtcgtact tcaccgggtt gcgccgctgc
     1261 tcgccgatct gcgcgatcag ctcgccgctg ttcgtataga cgcgcaacgg cacctggagc
     1321 tcgttgttct tcatcgccga gaccgacgga agcgtcggtt cgaggtagac gtagctgcag
     1381 acaaacgcgt aagcggccgc aagtgtgatc gccgtcacac ccatgaccac catgacgccc
     1441 aatcgaaagt agtttgactt cacagaatgc gggtctcgtc ggttgcggag gcttttggcc
     1501 gtatgcatag taaccgtcgt cgatatgcat actcatcgaa aggtggggac gcaaagcttc
     1561 cggtctaagg gctcaagatt ggcctacgac agcggggttg ccggctcggg ttccacgcct
     1621 gggttgatca gcaaactgcc ccactggttc caatcaaccc tcggtccggg atgcccgtct
     1681 ccggccgtgc gggtgtcagg gggatatccc cgggaatccg ccagtttcgt gagcagcgtc
     1741 tcgttcaatt taacttcacg ctgatatata gtgtctcggg tttaagattg gtcgcacggt
     1801 tctcgttcta acacactgaa aggactgcca aagtgctgtt cctcacgcgc aagtatcgtc
     1861 cgttactcgg acttgacata acaacttcgt cggtcaagtt gatcgagctc gtgacgagtg
     1921 gcggtgccta tcgcgtcgaa tcgtacgcgg cagagccaac cccacagaac gccatgaacg
     1981 aaaaagccat cgtcgacgcg gaagcggtcg gtgaggcaat tcgccgtgcc gtcaaacgtt
     2041 ccggcgcagg cgccaaggaa gttgccatcg caatctccgg cgacgctgcc atcaccaagg
     2101 tcatccagat gccgcgcagt ctgcgcgagc gcgatctcga agctcaggtc gagatgcagg
     2161 ctgaccaata cattcccttc ccgatggacg aagtcagcta cgacttcgaa gtgttaggcc
     2221 catcggagaa agatccagag accaacgacg tcctgctggt cgctacccgc actgaaaacg
     2281 tggaacagcg ccaggccgcg gtcaacgcgg cgggtctcac cgccaagatc gtcgacgtcg
     2341 aagcgttcgc cctcgagaac gcgtgccggc tcatgacgca tcagatgccc gacggcggca
     2401 tcgatcgcac gatcgccgtc gtggacttcg gcgcgagcag caccacgttc agcgtgctgc
     2461 gcaatctcaa ggttgtttac acgcgtgact tcgcgttcgg cgggcagcag ctcaccgaag
     2521 aaatcatgcg tacctacggg ctctcgatgg aagaagccgg ccgcgccaag aaagaaggcg
     2581 gattgccggg caatttccag gccgaagtgc tcgacccgtt cattgacgac atgacgcagc
     2641 aggtgagccg ctcgctgcag ttctatctcg cttcgggctc tggccgcgat caaccggaga
     2701 agattctcat ctgcggtggt tgcgcgaaca tttccggcgt ttccgacgtc atctcgagtc
     2761 gcgttggaat ttcggccgag aagggcgacc cgctcggtca gatgaaactc tcgtcccgcg
     2821 ccaaggcgca ggcggttgcg cgtgacgcca ctgcactgct cacggcctgt ggactcgcac
     2881 tgcggagctt cgacaactaa tgcctcgcat aaacctactt cc
//
LOCUS       DATDEU010000593         3642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1576271, whole genome shotgun sequence.
ACCESSION   DATDEU010000593 DATDEU010000000
VERSION     DATDEU010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576271"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VIV63_14250"
     CDS             complement(<1..915)
                     /locus_tag="VIV63_14250"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEY6125808.1"
                     /translation="MRTLVWFRGKDLRIADHAPLRSALPSAANPGSEVIPLFVLDDHF
                     FRPEAARRIPFRMQFLLESIASLAANLEHLGSKLILVDGRSAEQVPKLAERWKVDRVV
                     AQSWVAPIGRERDRQIGKALRVPFELFDSETLCPPGKLRTGAGEPYSVFTAFARAFAR
                     DIRVEAPAPAPRVLPPIPEDIRTRGVPLPTLESLGLTHNPQVQAGGERNARTRLKDWL
                     SRDAKDYDELRNRMDLSGTSRLSADLKFGTLSVRTVWQAVDRRLRELSPDALRVYQNE
                     LLWREFAHSTLFDRPRLLEETFRREWRDF"
     gene            complement(939..1814)
                     /locus_tag="VIV63_14255"
     CDS             complement(939..1814)
                     /locus_tag="VIV63_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY6125809.1"
                     /translation="MATENAPLSLDKTNAEKRGERPHWVLDDAVYSISALLLSVILIH
                     SIWTLIIRPRAEAVLSQRVTVTQAGSSVKAQVLQLRSIYVILKDHEQELCIILCMWSL
                     LLLARQAQDTRLARQLLDRDYVKIGDGQVVLPEDARVYSRPLEGLPAAEQSRFLPRLL
                     MVAYNRFGATRSVQDAAEAVRDECDFETASMDTKLSMVRFTAWAIPAVGFVGTVRGIG
                     AALQEAQGAMGGDISGVTMGLGVTFNATLVALVSCIIVMFWMHQLQQFQDRLVLDSRT
                     YVDRQLLRKMRVVSK"
     gene            1990..>3642
                     /locus_tag="VIV63_14260"
     CDS             1990..>3642
                     /locus_tag="VIV63_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6125810.1"
                     /translation="MSIALDQVTKRYQDQPVVNDVSIEVGEGEFFVLLGPSGSGKSTL
                     LRAIAGLTGIDHGRISLHGRDVTHVRARDRGVGLVFQNYALFRHMTVADNIEFALRVR
                     RMKAVDRRARRKELLKLVALEGMDERLPGQLSGGQQQRVAVARALAHKPQVLLLDEPF
                     GALDLKIREELRRTIRSVQRELGITTVLVTHDQEEAFALADTIGVMNHGRLLEVGNPN
                     DLYAQPATRFVATFLGAANLLLARQGADGIRFGATPVNAEIAEPVHGGREHEVVAVLR
                     PEEVEIASTREQLVSNYIARGAVEEIVFTGALERLRVRLLDEPSAAQLLGPSGSGDAL
                     LEITRTQPERRGLPLAQGTQIALGARRLHILPTPLSSFLACGATEADAAALAREPLLE
                     SLSTRMKTRVGLRAIDGLTGTPAPFAGVAAIAGGENAASTVEWLLRHGASEVLVLPRN
                     APSPTRVFIHWTDEAARRATLAVAASLLRHVSAEAVYLGIVPEGGRASGRPAPMRALL
                     DARSEAQQSHGLEMRTELAIGETVSELTRNLSAGTAQMLVLGI"
BASE COUNT          629 a         1186 c         1225 g          602 t
ORIGIN      
        1 aaagtcgcgc cattcgcgcc gaaacgtttc ctctaacaat cgagggcggt cgaacagcgt
       61 ggagtgcgcg aactcgcgcc acaagagttc gttctggtac acgcgcaacg cgtcgggcga
      121 cagttcgcgc aagcgccggt cgaccgcctg ccagaccgtg cgcacagaca gcgttccgaa
      181 cttgaggtcc gccgataacc ggctggtgcc ggaaagatcc atgcggttgc ggagttcgtc
      241 gtagtccttc gcatcgcgcg acagccagtc cttgaggcgg gtgcgtgcgt ttcgctcgcc
      301 gccagcctgc acctgcgggt tgtgcgtcag gccaagagat tcgagcgtgg gcagcggaac
      361 gccgcgtgtg cggatgtctt cagggatcgg cggaaggaca cgaggcgccg gcgccggggc
      421 ttcgacccgg atgtcgcgcg cgaatgcgcg tgcgaatgcg gtgaacacag agtagggctc
      481 gccggcgccg gttcgcaatt tgccgggcgg gcacaaggtt tcgctgtcga acaactcgaa
      541 cggcacacga agtgccttgc cgatctgccg atcgcgctct cggccgatgg gcgcgaccca
      601 gctctgggcc accacgcgat ccaccttcca ccgctccgcg agtttcggca cctgctcggc
      661 gctgcgccca tcgacgagga tcagcttcga gcccaggtgc tcgagattcg cggcgagcga
      721 ggcaatggac tcgagcagga actgcatgcg gaagggaatg cgccgggcgg cctcgggccg
      781 gaagaaatgg tcgtcgagca cgaacagcgg gatgacctcg ctgccggggt tcgcggcgga
      841 cggcaaagcc gagcgcagcg gcgcgtgatc cgcgatgcgc aggtccttgc cacggaacca
      901 gacgagcgtg cgcactagct agtcgtgcgc gcgcaggttt acttcgagac cacccgcatc
      961 ttgcgcagca gctggcggtc gacgtaggtg cggctgtcga gcaccaggcg atcctggaac
     1021 tgctgcaact ggtgcatcca gaacatgacg atgatgcagg agacgagggc gacgagcgtg
     1081 gcgttgaacg tgacgccgag gcccatggtg acaccggaga tgtcgccacc catggcgccc
     1141 tgcgcttcct gaagcgccgc gccgatgccg cgcaccgtac cgacgaagcc cacggccggg
     1201 atggcccagg cggtgaaccg caccatggag agcttggtgt ccatgctcgc ggtttcgaaa
     1261 tcgcattcgt cgcgaaccgc ctcggcggcg tcctgcacgc tgcgcgtggc gccgaaccgg
     1321 ttgtacgcca ccatgagcag ccgcggcagg aagcggcttt gctcggcggc tggcaatcct
     1381 tcgagtggcc gtgagtagac gcgcgcgtct tcgggcagca ccacctgtcc gtcgccgatc
     1441 ttcacgtagt cgcgatcgag gagctggcgc gcgaggcgcg tgtcctgggc ctggcgggcg
     1501 agcagcagca gcgaccacat gcagaggatg atgcagagtt cctgctcgtg gtccttgagg
     1561 atgacgtaga tggaacgcag ctggagcacc tgtgccttca cgctgcttcc ggcctgggtg
     1621 accgtgaccc tctgcgacag cacggcctcc gcgcggggac ggatgatcaa cgtccagatg
     1681 ctgtggatga ggatgaccga cagcagcagg gcgctgatgc tgtacaccgc gtcatccaga
     1741 acccagtgag gccgttcgcc acgcttctcc gcgttggttt tgtccaggct aagaggcgcg
     1801 ttctcggtgg ccattcaggt tttctcgttc ccgggaagag ggcaaaaagt gtactcgtta
     1861 gtcggcgggg taaccgatta agttccgcgg ccttataacc gtttgtgacc gcattgtaac
     1921 cagcgtgtta ttcgggccaa tacccccgcc tgatagcatc acggccggtt ttcacccagc
     1981 ccgcctcgca tgtccatagc actcgaccaa gtcactaaac ggtatcagga ccagcccgtc
     2041 gtcaacgacg tctccatcga ggtgggagag ggcgaattct ttgtactgtt gggcccgtcg
     2101 ggcagcggca agagcacgct tctgcgcgcg atcgcgggcc tcaccggcat cgaccacggc
     2161 cgtatttcgc tgcacggacg tgacgttacg cacgtgcgcg cgcgcgaccg cggtgtcggc
     2221 ctggtgtttc agaactacgc gttgttccgg cacatgaccg tcgccgacaa catcgagttc
     2281 gccttgcgtg tccgccgcat gaaagcggtc gaccgccgcg cccggcgcaa ggaattgctc
     2341 aaactggtcg cgctcgaagg catggacgag cggcttcccg gacaactgtc gggcggtcag
     2401 cagcagcgcg tcgccgtggc gcgtgcgctg gcacacaaac cgcaggtgct attgctcgat
     2461 gaaccattcg gcgccctcga cctgaagatc cgcgaagaac tccggcgcac catccgctcc
     2521 gtgcagcggg agctcggcat cacgacggtg ctcgtgacac acgaccagga agaggcattc
     2581 gcgctggccg acacgatcgg ggtcatgaac cacgggcgcc tgctcgaggt gggcaatccg
     2641 aacgacctgt atgcgcaacc ggccacgcgc ttcgtcgcca cgttcctggg cgcggccaac
     2701 ctgctgctgg cgcgccaggg tgcggacggc atccgcttcg gagccacacc ggtcaatgcc
     2761 gaaatagccg agcctgttca tggcggccgc gaacacgaag tggtggccgt gctgcgaccc
     2821 gaggaagtcg agatcgcctc gacgcgcgaa caactggttt cgaattacat cgcgcgcggc
     2881 gcggtggaag aaatcgtatt cacgggcgcg ctcgaacggc tgcgcgtccg cctgctcgac
     2941 gaaccgtccg ccgcgcaatt gctggggcct tcgggcagcg gagatgcgct gctggagatc
     3001 acgcgtacgc agccggagcg ccgcggactg ccgctcgcgc agggcaccca gatcgcgctc
     3061 ggcgcccggc gcctgcacat attgcccacg ccgttgtcga gcttcctcgc ctgtggcgcc
     3121 accgaggcgg acgcagctgc gctggcgcgc gaaccgctgc tcgaaagtct ctccacgcgc
     3181 atgaagacgc gtgtcggatt gcgcgcgatc gacggactca cgggcacacc ggctcccttc
     3241 gcgggcgtag ccgccatcgc gggcggcgag aatgccgcgt cgacggtgga atggctgctg
     3301 cgccatggcg cctccgaagt gctggtgctg ccgcgtaatg cgcccagccc gacccgcgtg
     3361 ttcattcact ggaccgatga agcggcgcgc cgcgccacgc tcgcggtcgc cgcgagcctg
     3421 ctgcgtcatg tgtcggccga ggcggtgtat ctcggcatcg ttcccgaggg cggtcgcgcc
     3481 agtggccggc ccgcgcccat gcgcgcgctg ctcgatgccc gttccgaagc acaacagagt
     3541 cacggtctcg agatgcgcac ggaactcgcc atcggcgaga cggtgagcga gctcacgcgc
     3601 aatctctcgg ccggcaccgc gcagatgctc gtgctcggca tc
//
LOCUS       DATDEU010000594         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_701775, whole genome shotgun sequence.
ACCESSION   DATDEU010000594 DATDEU010000000
VERSION     DATDEU010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701775"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VIV63_14265"
     CDS             complement(<1..718)
                     /locus_tag="VIV63_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6125811.1"
                     /translation="MAQMNMIQALNSAMDVMMARDPSVVVMGEDVGYFGGVFRVTDGL
                     QRKYGDQRVLDSPISEGGIVATAIGMAVNGLKPVAEIQFADYIYPAVDQIASELARIR
                     YRSAGDFSSPVVIRAPCGGGIRGGQTHSQSPEAIFTHICGVKVVMPSNPYDAKGLLIA
                     AIEDDDPIIFFEPKRIYNGPFDGDPNKPAVPWSTHTKGNVPEGHYKVPIGKCDIVRPG
                     EQVTIVTYGTMVYVCEAAAKK"
     gene            complement(723..1985)
                     /locus_tag="VIV63_14270"
     CDS             complement(723..1985)
                     /locus_tag="VIV63_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6125812.1"
                     /translation="MPRLHVPQPPTRPGDKPDFSYVELSPAGAINKPDVNARARDMEE
                     VSVGLVRVLDDKHEAVGQWNPRLEAEDLQVGLRHMLLTRIFDDRMQRTQRQGKISFYM
                     RCFGEEAVGVAQAMALQPGDMLFPSYRQQGIYMVRGKPLVELMCQLLSNTRDMCKGRQ
                     LPVMYHWNAGRIFSISGNLTTQFPQAVGWAMAAAIRNEDHIACTWIGEGSSAEADFHH
                     GIIFAAVHQAPVIMNIVNNQWAISTFQGTAGGEQRSFAARGPGYGIAGIRVDGNDFLA
                     VYSVTQWAAQRARTGGGPTLIELVTYRAAAHSTSDDPSRYRPKDDYEHWPLGDPVDRL
                     KNHLIARGEWSEKQHKELHAELDAEVVKAWKEAVSFGALNEGPRLDRHLMFEDVYKDF
                     PENLRRQSEQLAEEIKLNAELGLAAKAE"
     gene            complement(2060..>2458)
                     /locus_tag="VIV63_14275"
     CDS             complement(2060..>2458)
                     /locus_tag="VIV63_14275"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY6125813.1"
                     /translation="GVFWKVHADLSGDLRVRQQAILGLIRADRLFTVPGVLLIIATGV
                     SLALIANLPLLRTFWILWSLVLFGISGLAFGIAVGPLQKKLLANTTAGLAGNWNETEY
                     RALSKSWAVWGGIATAAPLIAVVLMVIKPA"
BASE COUNT          408 a          771 c          814 g          465 t
ORIGIN      
        1 gcttcttcgc cgccgcctcg cagacgtaaa ccatggtgcc gtacgtgacg atggtcacct
       61 gctcgcccgg tcgcacgatg tcgcacttgc cgatgggcac cttgtagtgt ccttcgggca
      121 cgttgccttt ggtgtgtgtg ctccacggca ccgcgggttt gttagggtcg ccgtcgaacg
      181 gtccgttgta gatgcgcttc ggctcgaaga agatgatcgg gtcgtcgtct tcgatggcgg
      241 cgatgagcag gcccttcgcg tcgtagggat tgctcggcat caccaccttc acgccgcaga
      301 tgtgcgtgaa gatcgcttcg gggctctgcg agtgcgtctg gccaccgcgg atgccgcccc
      361 cgcagggcgc gcggatcacc accggagatg agaaatcgcc ggcgctgcga taacggatgc
      421 gtgcgagttc gctcgcgatc tggtccacgg ctggatagat gtagtcggcg aactggattt
      481 ccgcgactgg cttcaatcca ttgaccgcca tgccgatggc cgtggccacg atgccgcctt
      541 ccgaaatcgg agagtcgagc acgcgctgat cgccatactt tcgctgcagc ccatcggtga
      601 cgcggaacac gccgccgaaa taacccacgt cttcgcccat gacgaccacg gacggatcgc
      661 gcgccatcat cacgtccatg gcggagttca gggcctgaat catgttcatc tgtgccatgg
      721 cttcactccg ccttggccgc gagcccgagc tcggcgttga gtttgatctc ttcggcgagc
      781 tgctcgctct gccggcgcag gttctccggg aagtccttgt agacgtcctc gaacatgagg
      841 tggcggtcga ggcgcgggcc ctcattgagc gcgccgaagg agacggcttc cttccaggcc
      901 ttgaccactt cggcgtcgag ctcggcgtgc agctccttgt gctgcttctc gctccattcg
      961 ccgcgcgcga tcaggtgatt cttcaaccta tccaccggat cgccgagcgg ccagtgttcg
     1021 taatcgtcct tcgggcggta acgcgaggga tcgtcgctcg tggaatgcgc cgccgcgcga
     1081 tacgtgacga gctcgatgag cgtcggcccg ccgccggtgc gcgcgcgctg ggccgcccac
     1141 tgcgtgaccg aatacacggc gaggaagtcg ttgccgtcga cacggatgcc cgcgattccg
     1201 tagcccggac cacgcgccgc gaacgagcgc tgctcgccgc ccgccgtgcc ctggaacgtg
     1261 gagatggccc actggttgtt gacgatgttc atgatgaccg gcgcctggtg cacggccgcg
     1321 aagatgatgc cgtggtggaa gtcggcctcg gcgctcgagc cctcgccgat ccaggtgcag
     1381 gcgatgtggt cttcgttgcg aatggcggcg gccatggccc agcccaccgc ctgcgggaac
     1441 tgggtagtta ggttgccgga gatcgagaag atgcggcccg cgttccagtg atacatgacc
     1501 ggcagctgcc ggcccttgca catgtcgcgc gtgttcgaga gcagctggca catgagctcg
     1561 accagcggtt tgccgcgaac catgtagatg ccctgctggc ggtacgacgg aaacagcatg
     1621 tcgccgggct gcagcgccat ggcttgggcc acgcccaccg cttcttcacc gaagcagcgc
     1681 atataaaagg agatttttcc ctggcgctgc gtgcgctgca tgcggtcgtc gaagatgcgc
     1741 gtgagcagca tgtggcgcag gccaacctgc aggtcctcgg cctcgaggcg cggattccac
     1801 tggcctacgg cctcgtgttt gtcgtcgaga acacggacga gtcctacgga cacctcttcc
     1861 atgtcgcggg cgcgcgcgtt gacgtcgggt ttgttgatgg caccggcggg cgacagctct
     1921 acatagctga agtcgggctt gtcgcccggc cgcgtggggg gctgcggaac gtgaagtctt
     1981 ggcattgcgc gatgatagcg cgggcgggaa gggagtgaag gcgcgcgtgg taattggttt
     2041 caggtgaaac cgatccggac tacgccggct tgatcaccat gaggaccacc gcgatcaggg
     2101 gcgcggccgt cgcgatccct ccccatactg cccaggactt cgacagcgct cggtactcgg
     2161 tctcgttcca gtttcccgcg aggccggcgg tcgtgttcgc cagaagcttt ttctgcagcg
     2221 gccccacggc aatgccgaac gccagcccgg aaatgccgaa caacaccagc gaccagagga
     2281 tccagaacgt gcggagcagt ggcaggttcg cgatcagcgc gagggaaacg ccggtggcga
     2341 tgatcagcag cacgccggga acggtgaaaa gcctgtcggc gcggatgagt ccgagaatcg
     2401 cctgctggcg tacgcgcaga tcgccactca ggtcggcgtg cactttccag aatacgcc
//
LOCUS       DATDEU010000595         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_557610, whole genome shotgun sequence.
ACCESSION   DATDEU010000595 DATDEU010000000
VERSION     DATDEU010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557610"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1101
                     /locus_tag="VIV63_14280"
     CDS             289..1101
                     /locus_tag="VIV63_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125814.1"
                     /translation="MAPTAREVSRADVPKPAASSDPFEIIRGLAKSAYEGDGRAQFTV
                     ARELDKCELTLSVVRKSGDPEAAIWGFANWTQAMKEWSIAEYRRCIRLVKEDPFADLP
                     PRAGGYTFRYWRERSAEAGYPLAVAERSLAGLAAPLPGLPANPKTRANALGELTQAAV
                     SGDPDVALTIGFRQFHSDNEVRFTSSAAWMLAACRLGADCSSSSNVVPFHMCYDPDFP
                     NCATDSNVEQMVSLALGPAKHADAYSQSQAIEEALRSRDDAALKPLLERLTN"
     gene            complement(1106..1933)
                     /locus_tag="VIV63_14285"
     CDS             complement(1106..1933)
                     /locus_tag="VIV63_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6125815.1"
                     /translation="MSMFKTEDGVRIFYKDWGTGQPLVFHHGWPLSADDWDAQLMYFL
                     AKGFRVIAHDRRGHGRSAQTFDGNEMDTYASDVSQLTAHLGLKGAVHIGHSTGGGEVA
                     RYVAKYGQDGRVAKAVLIGAVPPIMVKSDKNPGGLPIEVFDGFRSALVANRAEFYRAV
                     PSGPFYGFNRPGAKTLPGVVDNWWRQGMMGGIKPHYDCIKAFSETDFTDDLKKIEVPT
                     LVMHGDDDQIVPIADSALLSSKLLKKGTLKVYPGLGHGLCTINPDVVNPDLLAFIQS"
     gene            complement(1950..2780)
                     /locus_tag="VIV63_14290"
     CDS             complement(1950..2780)
                     /locus_tag="VIV63_14290"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HEY6125816.1"
                     /translation="MKEETFTGRGGTRLHMRSWRPAGTPRAAVVLCHGVLAHSGQYLW
                     SGEQLSAAGFAVYAYDHRGRGKSEGPRLFVNDVAEYTDDLGTFIDLVKTRDPGLKVFL
                     LGHSMGGVISCTWTLDHQKEIAGFICESFAFEVWAPKPVLSLVRFLGRVAPKLPVLKL
                     HEKDFTRDPLALEKLKSDPLTHGEVQPAGTVAALLKATDRMRAEFGSITLPLLIMHGT
                     KDKATVPAGSQLFYDKAGSSDKTLKLYEGHFHDLLNDLGKEKVMADVVSWINARAAVQ
                     "
     gene            complement(2792..>3264)
                     /locus_tag="VIV63_14295"
     CDS             complement(2792..>3264)
                     /locus_tag="VIV63_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6125817.1"
                     /translation="LLWRQSRAVSGPEVFDVDAGVVDAEATIAQARTLSAGNGRVGVM
                     GFSLGGLLAFLTAARTCVDAAVAYYGGRTEEFLNEAPDIDGPLQMHLAGNDEFMPKAA
                     HQQIVMTLADNAQVEIYSYDSCHHAFSRRGGSNYDAAAALLSRARALEFFKRHL"
BASE COUNT          596 a         1063 c          999 g          606 t
ORIGIN      
        1 cttccgccgc gcgcgccgcg gttgccggca gcaggcccgc ggccgcggcc caggcgcccc
       61 acttcaggag atcacggcga ggtctgcgca tttgttaggc tttctatcac accctcatcg
      121 ggcgacacta tccccgccat gaccctgccc ttgaagtttg ctcttgccgt agcgctgacc
      181 atcgtcgcgg catgtgctgc tctttatttg ttccgcgaac agcctgtcga agcgcctccg
      241 tccaaaggcg aggtgatgct cgcgccggct tcttcagtcg agccgtcagt ggcgcctacg
      301 gcccgcgagg tttcccgcgc ggacgttccg aaaccggccg ccagttcgga tcctttcgag
      361 atcatccgcg ggctggcgaa gagcgcgtac gaaggtgatg gccgggcgca gttcaccgtg
      421 gcgcgcgaac tcgacaaatg cgaactgacg ctctcggtag ttcgcaagtc gggcgacccg
      481 gaggccgcga tctggggatt cgccaactgg acgcaggcga tgaaggaatg gtccatcgcc
      541 gagtaccggc gatgcatccg cctggtgaag gaagatccgt ttgccgacct gccgccgcgc
      601 gcgggtggct acaccttccg gtactggagg gaaagatcgg cggaggctgg ctatccactg
      661 gcggtggcgg aacgatcact cgcgggactg gcggcgccgc ttcccggttt gccggcgaat
      721 cccaagactc gcgccaatgc actgggcgag ctgacacagg ccgccgtctc cggcgacccg
      781 gacgtcgcgc tcaccatcgg cttccggcaa tttcattccg acaacgaagt tcgcttcacc
      841 agttcggccg cgtggatgct ggccgcctgc agattgggcg ccgactgcag ctccagcagc
      901 aatgtggttc ctttccacat gtgctacgac ccggactttc cgaactgcgc cacggattca
      961 aacgtggaac agatggtgag tctcgcgctc ggacccgcga agcacgccga cgcgtactcg
     1021 cagtcgcagg ccatcgaaga ggcactgcga agccgggacg atgccgcgct caagcccttg
     1081 ctcgagcgcc tcacgaacta gatcctcatg actggatgaa cgccagcaga tccggattca
     1141 ccacgtccgg gttgatggtg cacagtccat gtcccaaccc cggatacacc ttgagcgtgc
     1201 ccttcttgag cagcttgctc gacaggagtg cggagtccgc aatcggcacg atctgatcgt
     1261 cgtcaccgtg catcacgagc gtcggcactt ctatcttctt gaggtcgtcg gtgaaatcgg
     1321 tttccgaaaa cgccttgatg cagtcgtagt gcggcttgat gccgcccatc atgccctggc
     1381 gccaccagtt gtcgacgaca cccggcagcg tcttcgcacc cgggcgattg aatccataaa
     1441 acggtccgga cggaacggcg cggtagaact ccgcacgatt cgcgactagc gccgagcgga
     1501 atccatcgaa cacttcgatg ggcaggccgc caggattctt gtcgctcttg accatgatcg
     1561 gcggcacggc gccgatgagc acggccttgg ccacgcggcc atcctgaccg tatttggcga
     1621 cgtaacgcgc cacttccccg ccgcccgtgg aatggccgat gtgcacggcg cccttgagac
     1681 cgaggtgcgc cgtgagctgc gagacgtcgc tggcataggt gtccatctcg ttgccgtcga
     1741 aagtctgcgc tgaacggccg tgaccgcgtc ggtcgtgcgc aatgacgcgg aaccctttcg
     1801 ccaggaagta catgagctgc gcgtcccaat catccgccga caacggccag ccatgatgga
     1861 acacgagcgg ctgcccggtg ccccagtcct tgtagaaaat cctgacgcca tcttcagtct
     1921 tgaacattga catgacccat tcctcctatc tattgaaccg cggcacgcgc gttgatccag
     1981 ctcaccacgt cggccatgac tttttccttg cccaggtcgt tgagcagatc gtggaaatgc
     2041 ccttcgtaga gtttcagcgt cttgtcgctc gagccggcct tgtcgtagaa aagctggctg
     2101 cccgcgggca ccgtggcctt gtccttcgtg ccgtgcatga tgagcaacgg cagcgtgatc
     2161 gagccgaact cggcgcgcat tcggtccgtg gccttcaaca gcgccgcgac cgtgccggcg
     2221 ggctgcactt caccgtgggt cagaggatcc gacttcagtt tttccagcgc gagcggatcg
     2281 cgcgtgaagt ccttctcgtg caacttcagc acgggcagct tcggcgcgac gcgcccgaga
     2341 aagcgcacca gcgacaacac gggcttgggc gcccacacct cgaacgcgaa actctcgcag
     2401 atgaaaccgg cgatctcttt ctgatggtcg agcgtccagg tgcaggagat caccccaccc
     2461 atgctgtggc ctaacaggaa gaccttgaga ccgggatcgc gcgttttgac gaggtcgatg
     2521 aacgtcccga ggtcatcggt gtactcggcc acgtcgttga cgaacaggcg cgggccttcg
     2581 gacttgccac gcccgcgatg atcgtaggcg tagaccgcga acccggcggc cgacaactgc
     2641 tcgcccgacc acaggtattg cccgctgtgc gccaggacac cgtggcagag cacgacggct
     2701 gcgcgcggag ttcccgccgg ccgccaggag cgcatgtgga ggcgagtgcc gccgcgaccg
     2761 gtgaatgttt cttctttcat ggaggacctc gttagagatg gcgtttgaaa aattcgagtg
     2821 cccgcgcgcg tgacagcaag gcagccgcgg cgtcgtaatt gcttccgccg cgccgcgaga
     2881 acgcgtgatg gcaactgtcg taactgtaga tctccacctg ggcattgtcc gcaagtgtca
     2941 tgacgatctg ttggtgggcg gcctttggca tgaactcgtc gttcccggcg agatgcatct
     3001 ggagtggccc gtcgatgtcg ggcgcctcgt tgagaaactc ttcggtacgg ccgccgtaat
     3061 acgccaccgc ggcatccacg caggtgcgcg cagccgtgag aaacgcgagc aagccaccca
     3121 gagagaaacc catgacaccg acgcgaccat taccggccga cagagtacgc gcttgcgcga
     3181 tcgttgcttc ggcatcgacc acgccggcgt ctacgtcaaa cacctcaggg ccgctcaccg
     3241 ctcgcgattg ccgccacaac aggt
//
LOCUS       DATDEU010000596         3067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1868616, whole genome shotgun sequence.
ACCESSION   DATDEU010000596 DATDEU010000000
VERSION     DATDEU010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868616"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VIV63_14300"
     CDS             <1..244
                     /locus_tag="VIV63_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125818.1"
                     /translation="YQVTFDGAAQANNTVVGQLRDLGGPFAVNGTVTLSAPKSYLVQG
                     FITGRTAEAERLVREITLGSPPDASGRSAFSFEGSY"
     gene            complement(245..>3067)
                     /locus_tag="VIV63_14305"
     CDS             complement(245..>3067)
                     /locus_tag="VIV63_14305"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY6125819.1"
                     /translation="SWPRLESLIAGMLPKRAEWLPNLPQLSGEALVPKIEASLAAIVS
                     EELAKVAASFPDSFIEQATALARFAAQYVDAAKLPDVASWRDATSALTGTLPDLARWR
                     GIAKLALTAEGNARKTLNKNDGIPADSVHGKALRDDWVERLRLMGADHLAALVAIRGL
                     PDPVVPDKARGALEALAKLLLLAATQLTRLFDLHGECDHVEIAGAARRALTEDSSPTP
                     LAERLGTRLMHILVDEFQDTSRDQYDLLRTLTQDWSAGDGRTLFLVGDPMQSIYGFRN
                     AEVGRFSTVRAAGLAGLKLTTLELRRNFRSAPALVHWCNEVFSRVFPAADDVRRSAVR
                     HLASVAARTNLEGAPHLYRVEGDCGPAAEAEHAADLIAELKRTRPSETIAVLASARPH
                     LRAIRECLAARSVPFIGVKLEPLADVSVVRDLEALVRAIDSPLDRVAWLAVLRAPFVG
                     LTLTDLTVVSDAAGDATILSALLGDIHGLSADGVERLLRAKPLLLAAWAEREREPRAH
                     MAERVWLALCGASAAKTGEAAHARRFLAALDEEDRKRARGRQLDLDRLMGRLYAEDPA
                     QPDAVSLMTIHGAKGLEFDHVFVVGVGLRGRGDDPRLLNWLEMPREQGGDHLVMAPIR
                     YRGDEEDDEDDAINQYLKELHRERARAERARQAYVALTRAKRSLHLFVHPRVKETDGG
                     LDFGAETNSLLHNLWPAIGADAASFHALGGDAEPDAGAIAPAMTHTRQRLHRRPSPLA
                     PPPDVLAQGEILPSSTEQDEIEFSWVRQTARRVGTVVHEALERFGRAGPPSVEELPRM
                     RVRLESRLQALGIEAARAREGAEFALRALHATLADERGRWLFDVRHTEAYSELALSGV
                     RGSHIVNAVIDRTFITADGTRWVVDFKTSPHEGGDVAAFLDAEAVRYTAQLARYAHLA
                     RQLGPQPVRTGLYYPLLAAWREVDVN"
BASE COUNT          480 a         1033 c         1048 g          506 t
ORIGIN      
        1 gtatcaggtc accttcgacg gcgccgcgca ggccaataac accgtggtgg gccagctgcg
       61 cgatctcggc ggaccgttcg cagtcaacgg cacggtgact ctctccgctc cgaaatcgta
      121 tctcgtgcag ggcttcatca cgggacgcac ggcggaagct gaacgcctgg tgcgcgagat
      181 cacgctcggt tcgccgcccg acgcctcggg ccgcagcgcg ttcagcttcg aaggctccta
      241 ctagctagtt gacgtcgact tcgcgccagg ccgcgagcaa cgggtagtac aggcccgtgc
      301 ggaccggttg cgggccgagc tggcgcgcga gatgcgcata cctcgcgagc tgtgccgtgt
      361 aacgcacggc ttccgcgtcc aggaatgccg ccacgtcgcc gccttcgtgc gggctcgtct
      421 tgaaatccac cacccagcgg gtgccatccg ccgtgatgaa cgtgcgatcg atcaccgcgt
      481 tgacgatgtg agagccgcga acgccggaga gcgcgagttc ggaatacgcc tcggtgtgcc
      541 gcacgtcgaa caaccagcgg ccacgctcgt cggcgagggt ggcgtgcagg gcccgtaacg
      601 cgaactcggc gccctccctg gcacgcgcgg cctcgatacc cagcgcctgc agccgtgatt
      661 cgagccgcac tctcatgcgc ggaagttctt cgacggacgg cggtcccgcg cggccgaatc
      721 tttcgagcgc ttcgtgcacg accgtgccga cgcgccgcgc cgtctggcga acccagctga
      781 attcgatttc gtcctgctcc gtgctcgaag gcaggatttc gccttgcgcc agcacgtcgg
      841 gcggcggcgc gagcggcgaa ggccggcggt gcagccgctg gcgcgtgtgc gtcatggctg
      901 gcgcaatggc cccggcatcc ggctccgcgt cgccgccgag agcatggaag ctggcggcat
      961 cggcgccgat cgcgggccac aggttgtgca gcagcgagtt ggtctcggcg ccgaagtcga
     1021 gaccgccgtc cgtctccttg acgcgcggat gcacgaacag atgcagcgag cgcttggcgc
     1081 gcgtcagcgc gacgtacgcc tggcgcgcac gctccgcacg cgcccgttct cgatgcagct
     1141 ccttgaggta ctgattgatc gcgtcgtcct cgtcgtcttc ttcatcaccg cggtaacgaa
     1201 tcggcgccat cacgagatgg tcgccgccct gctcgcgcgg catctcgagc cagttcaaca
     1261 gccgggggtc gtcaccgcgg ccacgtaacc ccacgccgac cacgaacacg tggtcgaact
     1321 cgagtccttt cgcgccatgg atcgtcatga gcgagaccgc gtccggttgc gccggatctt
     1381 cggcgtagag gcggcccatg agccggtcga gatcgagctg acggccgcgc gcgcgcttgc
     1441 ggtcttcctc gtcgagcgcc gcgaggaaac ggcgggcgtg agcggcctcg ccggtcttcg
     1501 ccgcggatgc accgcagagg gccagccaga cccgttcggc catgtgggcg cgcggctcgc
     1561 gttcgcgctc ggcccaagcc gccagcaaca ggggtttggc acgcagcagc ctttcgacac
     1621 catcggcgga cagtccgtga atgtcgccta acaaggcaga gaggatggtc gcgtcgcccg
     1681 cagcgtcgct gactaccgtg agatcggtga gagtgaggcc gacgaacgga gcgcgcagca
     1741 cggcgagcca cgccacgcga tcgagtggcg agtcgattgc gcgcaccagc gcttccagat
     1801 cgcgcaccac cgacacgtcg gccaggggtt cgagtttcac gccgatgaac gggacgctgc
     1861 gcgcggccag gcattcgcga atggcgcgca gatgcgggcg tgcgctcgcg agtaccgcga
     1921 tggtttcgga ggggcgcgtg cgtttcagtt ccgcgatcag gtccgcggca tgttcggctt
     1981 cggctgccgg tccacagtca ccttccacgc gatagagatg tggtgcgcct tcgaggttcg
     2041 tgcgcgccgc gacgctggcc aggtgccgca cggcgcttcg gcgcacgtcg tcggccgccg
     2101 gaaacacgcg cgagaacacc tcgttgcacc agtgcacgag tgccggcgcg gagcggaaat
     2161 tgcgccgcag ttccagggtc gtcaatttca ggccggcaag tcccgccgca cgcaccgtcg
     2221 agaagcgccc cacttccgcg ttgcgaaagc cgtagatgga ctgcatggga tcgccgacga
     2281 ggaacaaggt gcgtccgtct ccggcggacc agtcctgcgt gagcgtgcgc aacaggtcgt
     2341 attgatcgcg cgacgtgtcc tggaattcgt cgacgaggat gtgcatcagc cgtgtgccga
     2401 ggcgctcggc gagtggcgtc gggctgctgt cctcggtgag cgcgcgccgc gcggcgccgg
     2461 cgatttcgac gtgatcgcat tcgccgtgca ggtcgaacag gcgtgtgagc tgcgtcgccg
     2521 cgagcaacag cagtttcgcc agtgcctcga gcgcaccgcg cgccttgtcg ggcacgaccg
     2581 gatcgggcaa tccgcgaatg gccaccagcg ccgccaggtg atcggcgccc atgagccgca
     2641 ggcgttctac ccagtcgtcg cgcagcgcct tgccgtgaac gctgtcggcg gggatgccgt
     2701 cattcttgtt gagagtcttg cgcgcattgc cttcggccgt cagcgccagc ttggcgatgc
     2761 cacgccagcg cgcgagatcg ggcaacgttc cagtcagtgc ggacgtcgcg tcacgccagc
     2821 tcgcgacatc ggggagcttc gcggcgtcca cgtattgcgc tgcgaagcgc gccagcgcgg
     2881 tcgcctgctc gatgaaactg tccgggaacg atgctgccac cttcgcgagt tcttcactga
     2941 cgatggccgc gaggctcgct tcgattttcg gcaccagcgc ttcaccggac aattgcggca
     3001 gattgggcag ccattccgcg cgcttcggca acatgccggc gatcagcgat tcgagtcgcg
     3061 gccagct
//
LOCUS       DATDEU010000597         4733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1577730, whole genome shotgun sequence.
ACCESSION   DATDEU010000597 DATDEU010000000
VERSION     DATDEU010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577730"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VIV63_14310"
     CDS             <1..894
                     /locus_tag="VIV63_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125820.1"
                     /translation="ICYLDRGAGAAIRRARTRLPGGGDNPVAVIRVPRERMVGSGIAS
                     SLMHEVGHQAAALLDLVASLRATLGGLQRGGQDADVWRLWDRWISEIVADFWSVARVG
                     ITSTLGLIGVVSLPPPFVFRVNLADPHPVPWIRVRLSCAIGAALYPHAQWARLSDLWR
                     SLYPVEGLDPARRELIARLEDNMPAFVEMLVGHRPASLRGASLREALKVDERQPARLK
                     QLFLNAQRRPQSLRQWPPTLAFAVVGQARADGLLSPEAESATVSKLLRHWALADTLRS
                     AESCAAAHHPFQAGREFAPAT"
     gene            914..2431
                     /locus_tag="VIV63_14315"
     CDS             914..2431
                     /locus_tag="VIV63_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125821.1"
                     /translation="MSVTSEELPRAVRIDGIVRTTAQSRTAKTDSQAFWVAIRNRTDA
                     IGFQRYSEFIHELMCGEGQGGAPRCGTTDVSTNDSAPGHGSYGAPSLGERRDELRTRP
                     SIYGMDAYQVLLLATQAFVVFEAGVVIASARLSGGGVVPGFDGPGDALESRIDRTVTL
                     AEIRAELEDYLSASAGRGLPYLSRIVGTLVAPNSDGETEQVPPWCRGILRNRFSCPSM
                     LELIWSYWHEEGMLVQSLNALSLRFQNRRIGDRDPLAQLEIDALRPLNNLLWGYIQDE
                     QNRLTLVRRAYEYDHHYGIKLVGKAVPTLRSADSRSKFIESFHNLLYRSAKFYQEDAD
                     TNVVADGFPLLNALKEVHLILAEGAHNQFGDLPWTARVEMLIQQWLLARPEIREFIRG
                     RVMVPYREDWMGQVDAMKKLQGWTDVSITHFHDLGRYGEQLLLSVRYDDWSNVADQEI
                     AKAWVRYWKPEVQGYLHAYRSATGQDLTVEPVDSNSPSVHLRTRLEDQRRRGPGG"
     gene            2440..3492
                     /locus_tag="VIV63_14320"
     CDS             2440..3492
                     /locus_tag="VIV63_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6125822.1"
                     /translation="MLDFTSALYLGLRHPSTSLAPWPAMSAGRPAALAETTGAVPVAA
                     AFAQIMGCETGTLLPSTLHLFWDLFASLPRQSVFYVDADAYPVARWGVERAVARGAQR
                     RTFAHLDAASLRQRLARASRAGELPVVVTDGFCPVCARPAPIADLLAAVREYGGLLVI
                     DDTQALGVLGEHGGGSLRHSRVSANDVLVAASLAKGFGVPAAMLAGSAAHVDRFRRRS
                     LTRVHCSPVTVAVVRAAEHALRVNESHGDVLRARLMRRVREFRAALAPTCASVRGIAF
                     PVQTLDCALSRGEGARVLHRELLERGVRAVLHGAADPRLSFIITARHSDADIHQAARE
                     VSRCLNHVQGMKRGVR"
     gene            3489..>4733
                     /locus_tag="VIV63_14325"
     CDS             3489..>4733
                     /locus_tag="VIV63_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6125823.1"
                     /translation="MKNYSDNEFDFEFDFEFDFESPGSPPPRAGTRGAVGGGSSTRRP
                     PKLIEFVMPDKFPFKGTTMPSAHDNAIKRVAHLLVFSSTIGTPIRDVQVIGHADKAGS
                     DATNMSVGLDRAKNVRQKIINEMNRIRPGSSAGVLITPLSRGESSPVDRTGTPGGNAL
                     NRRVQIMLPATCQTLFAQLDLQTFPGSAVLGVAAHPNVTDKPERLRMVGAAVSEILPR
                     LEFRAREALAGRVVKSRPLGAAASAALRADVLGLSRLQLKLFNQYLPGATGGFSQEKL
                     MGCFQKFANGEMRSPHSDFPGQGVNEPDSQFFFLFAEFAFLCHDSGIAKGSWLRALRA
                     MVGAQEIFMHVYRKSPPLTPPAVGAALPAPGAPATTPLGDFGNANFTPVGASITRGRG
                     QSDAARKKALRRKYERMGINQLY"
BASE COUNT          744 a         1571 c         1616 g          802 t
ORIGIN      
        1 atctgctacc tcgaccgtgg cgcgggtgcc gcgattcgcc gcgcacgcac gcgcctgccg
       61 ggcggcggcg acaacccggt ggcggtgatc cgcgtgccgc gcgagcgcat ggtgggcagc
      121 ggcatcgcct cgtcgctgat gcacgaagta ggtcaccagg ccgcggcgct gctggacctg
      181 gtggcatcgt tgcgcgccac gctcggcggc ctgcagcggg gcggccagga tgcggacgtg
      241 tggcgcctct gggatcgctg gatctcggag atcgtggccg acttctggtc ggtggcgcgt
      301 gtcggcatca cctcgacgct gggtctcatc ggcgtcgtga gtctgccgcc gccgttcgtg
      361 ttccgcgtga acctggccga tccgcatccg gtgccgtgga ttcgtgtgcg gctttcctgc
      421 gccatcggcg cggcgttgta tccgcatgcg cagtgggcgc ggctgtccga tctgtggcgc
      481 tctctgtatc ccgtcgaggg actcgatccc gcacgccgcg aactgatcgc gcggctcgaa
      541 gacaacatgc cggccttcgt ggagatgctg gtcgggcacc ggcctgcgtc gctgcggggt
      601 gcgtcgctgc gcgaagcgct gaaggtcgac gaacgccagc cggcacggct caagcaactc
      661 tttctcaatg cgcaacgtcg gccgcagagc ctgcgccagt ggccaccgac gctggccttc
      721 gcggtcgtcg gccaggcacg cgccgatggc ctgctcagcc ccgaggccga atcggccacc
      781 gtctccaaac tactgcgcca ttgggcgctg gccgacacgc tgcgctcggc cgagtcctgc
      841 gccgcagccc atcacccatt ccaggccggc cgcgaattcg cgccagccac ctgactacct
      901 gtaggaggac atcatgagcg ttaccagtga agaacttccc cgcgcggtgc gtatagacgg
      961 catcgtgcgg accacggccc agtcacgcac ggccaagacc gattcgcagg cattctgggt
     1021 tgcgatccgc aaccgcaccg atgcgatcgg cttccagcgt tactcggaat tcatccacga
     1081 gctgatgtgc ggagaaggcc agggcggcgc gccgcgttgc ggcacgaccg acgtgtcgac
     1141 caacgactcc gcgccgggtc atggcagcta cggcgcgccg tcactcggcg agcgccgcga
     1201 cgagctgcgc acgcggccca gcatctacgg catggacgcg taccaggtgc tgctgctcgc
     1261 cacgcaggca ttcgtggtgt tcgaagcggg cgtagtcatc gcgtcggcgc ggctcagcgg
     1321 cggcggtgtg gtccccgggt tcgacgggcc cggcgacgcg ctcgagtcgc gcatcgatcg
     1381 caccgtgacg ctggccgaga tccgcgcgga actcgaggac tatctgtccg cgagcgccgg
     1441 ccgcgggctg ccgtacctga gccgcatcgt tggcacgctg gtcgcgccga attccgatgg
     1501 cgagaccgag caggtgccgc cgtggtgccg cggcatcctg cgcaaccgct tcagctgccc
     1561 gagcatgctg gagctcatct ggtcgtactg gcacgaggaa ggcatgctcg tgcagtcgct
     1621 gaacgcgctg tcgctgcgct tccagaaccg tcgcatcggc gaccgcgacc cgctggcgca
     1681 gctcgagatc gatgcgttgc gtccgctgaa caatctgctc tggggctata tccaggacga
     1741 gcagaaccgt ctcacgctgg ttcgccgcgc gtacgaatac gaccatcact acggcatcaa
     1801 gctcgtcggc aaggcggtgc ccacgctgcg ctccgcggac agccggtcga agttcatcga
     1861 gagcttccac aacctgctgt accggtccgc gaagttctac caggaggacg cggacaccaa
     1921 cgtggtcgcc gacggcttcc cgctgctcaa cgcgctcaag gaagtgcacc tgatcctcgc
     1981 cgaaggcgcg cacaaccagt tcggcgatct gccgtggacg gcgcgcgtgg agatgctcat
     2041 ccagcagtgg ctgctggcgc gtccggagat ccgcgaattc atccgcggcc gcgtcatggt
     2101 gccgtaccgc gaagactgga tgggccaggt cgacgccatg aagaagctgc agggctggac
     2161 cgacgtgagc atcacgcact tccacgacct cgggcgttac ggcgaacagc tgctgctgtc
     2221 ggtgcgctac gacgactgga gcaatgtcgc cgaccaggag atcgccaagg cctgggtgcg
     2281 ttactggaag ccggaagtgc agggctatct acacgcctat cgttccgcga ccggccagga
     2341 cctcaccgtg gagccggtcg actcgaactc gccgtccgtg catctgcgca cgcggctgga
     2401 agatcagcgt cgccggggtc cgggcgggta gtcacttgaa tgctcgactt cacatccgcc
     2461 ctgtacctgg gactgcgtca cccgagcacg tcgctcgcgc cgtggccggc gatgagtgcc
     2521 ggccgtccgg cggcgctggc ggaaaccacg ggcgccgtgc cggtagccgc ggcgttcgcg
     2581 cagatcatgg gctgcgaaac cggcacgttg ttgccatcga cgctgcatct gttctgggac
     2641 ctgtttgcca gcctgccgag gcagtcagtg ttctacgtgg atgcggacgc ctatccagtc
     2701 gcgcgatggg gcgtggagcg cgccgtcgcg cgcggtgcgc aacgacgcac gttcgcgcat
     2761 ctcgatgccg cttcgctgcg acagcggctc gcacgcgcga gtcgtgcggg agagctgccc
     2821 gtggtggtca ccgatggctt ctgtccggta tgcgcgcggc ccgcacccat tgcggatctg
     2881 ctcgccgcgg tgcgcgagta cggtggactg ctggtcatcg acgacacgca ggcgctgggt
     2941 gtgctcggag agcatggcgg cggcagcctt cgtcacagcc gggtgtccgc caacgacgtg
     3001 ctggtcgcgg cctcattggc gaaaggcttc ggtgtgccgg ccgccatgct cgcgggatca
     3061 gcggcgcacg tcgacagatt caggcgccgc agcctcacgc gcgtgcactg cagtccggtg
     3121 acggtggccg tcgtgcgtgc tgcggaacat gcgctgcgcg tgaacgaatc gcatggcgac
     3181 gtattgcgcg cgcggctcat gcggcgcgtc cgcgagttcc gcgccgcgct ggcgccaacc
     3241 tgcgcatcgg tgcgtggcat tgcgtttccc gtacagacgc tggattgcgc gctctcgcga
     3301 ggcgagggcg cgcgggtctt gcatcgcgag ctgctcgaac gcggggtgcg tgccgtactg
     3361 cacggcgccg cggacccgcg cctgagtttc atcatcacgg cccgccatag cgacgccgac
     3421 atccaccagg cggcgcgcga agtgtcgcgt tgcctgaacc acgttcaagg aatgaagcga
     3481 ggtgtgcgat gaaaaattac agcgataacg aattcgattt cgagttcgac tttgaattcg
     3541 acttcgagag tcccggcagt ccgcctcccc gggcgggcac gcgcggcgcc gtcggtggcg
     3601 gtagttcgac gcgccggccc ccgaagctca tcgaattcgt gatgcccgac aagtttccgt
     3661 tcaagggcac gaccatgccc tcggcgcacg acaacgcgat caagcgcgtc gcgcacctgc
     3721 tggtgttcag ttcgacgatc ggcacgccca ttcgcgacgt gcaggtgatc ggccatgcgg
     3781 acaaggccgg cagcgatgcg accaacatga gtgtgggcct ggatcgcgcc aagaatgtgc
     3841 gccagaagat catcaacgag atgaaccgca tccggcctgg ttcgagcgcg ggcgtactca
     3901 tcacgccgtt gtcgcgagga gagagcagtc ccgtggaccg gacgggtaca ccgggcggca
     3961 acgcgctcaa tcgtcgcgtg cagatcatgt tgcccgcgac ttgccagacg ttgttcgcgc
     4021 aactggatct gcagacgttt ccgggaagcg cggtgctggg ggtggcggct catcccaacg
     4081 tcaccgacaa gcccgagcgt ttgcgcatgg tcggcgccgc ggtcagcgaa attctgccgc
     4141 gactcgagtt ccgcgctcgc gaggcgttgg ccggcagagt cgtgaagagc aggccgctgg
     4201 gcgctgcggc gagcgccgcg ctgcgcgcgg atgtgctggg gttgtctcgg ctgcagctca
     4261 agctgttcaa ccagtatctg cccggggcga ccggcggttt ctcccaggaa aagctcatgg
     4321 gctgttttca gaaattcgcc aacggcgaaa tgcgcagccc gcactcggac tttcccggcc
     4381 agggtgtgaa cgagccggac agccagttct tcttcctgtt cgccgagttc gcgtttctct
     4441 gtcacgactc gggtatcgcc aagggttcgt ggctgcgtgc gctgcgcgcg atggtcggcg
     4501 cgcaagaaat tttcatgcac gtgtatcgca agtcgccgcc actgacgccg ccggccgtgg
     4561 gtgcggcgct gccggcaccg ggtgcgcctg ccacgacgcc gctcggcgac ttcggaaacg
     4621 ccaacttcac gcccgtcggc gccagcatca cacgcggtcg cgggcagtcc gacgccgcgc
     4681 gcaagaaggc gctgcgccgc aagtacgaac gcatgggcat caaccagctc tac
//
LOCUS       DATDEU010000598         4045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_849175, whole genome shotgun sequence.
ACCESSION   DATDEU010000598 DATDEU010000000
VERSION     DATDEU010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849175"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VIV63_14330"
     CDS             complement(<1..763)
                     /locus_tag="VIV63_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6125824.1"
                     /translation="MADLLQARGIVHAYAGKPALDGVDFTLRAGEIHALMGQNGAGKS
                     TLIKVLTGAEILQRGSIVLDGQRIAPTSNREAQRLGISTVYQEVNLCGNLSVAENIFA
                     GRQPLRRWTRGGGIDWRTLNARARTLLAGLDIDIDVRRELSSYSVALQQMVAIARAID
                     ISARVLILDEPTSSLDSDEVEKLFAAMRRLRESGMAIVFVTHFLDQVYAVSDRITVLR
                     NGRLVGEYPAAELDHAALVAAMVGRKTGAETAATPP"
     gene            complement(771..1763)
                     /locus_tag="VIV63_14335"
     CDS             complement(771..1763)
                     /locus_tag="VIV63_14335"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6125825.1"
                     /translation="MKRFIQMTLVTAAAALLVACSGGDKKAAADGSKAIVLGFSQIGA
                     ESEWRTANTTSIQSAAPEAGITLRFSDAQQKQENQIKALRSFIAQRVDVIAFSPVVET
                     GWDTVLREAKTAGIPVILTDRAVEVSDASLYLSLIGSDFVDEGRKAGRWLTEYYATGE
                     GKAAQGDIHIVELQGTVGSAPANDRKKGFAEIVGAEPRFKIIRSQTGDFTRAKGKEVM
                     EAFLKAEGKKINVLFAHNDDMAIGAIQAIEEAGLKPGTDIIIVSIDGVKGAFEAMIAG
                     KLNVTVECSPLLGPQLMAAVKDVVAGKEIPKRIVTEESVFPRETAAQVLPTRKY"
     gene            complement(1789..2976)
                     /gene="araD1"
                     /locus_tag="VIV63_14340"
     CDS             complement(1789..2976)
                     /gene="araD1"
                     /locus_tag="VIV63_14340"
                     /inference="COORDINATES: protein motif:HMM:NF040903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HEY6125826.1"
                     /translation="MLPSSQIAVSNMSWRVLRRARMNHGVVVVIVFSPVSSQLYNYGY
                     SSVAISGLLDINTNPMIPPLRLIQFLDESGQPRVGWVTDDGASVGSVGSFQSTSELVQ
                     AASSAAEPLDAFASRQAAPPSIPYLALLDGRRVLAPLTHPDPARCLVSGTGLTHYGSA
                     SARDQMHKKLAAETLSDSMQMFKWGVEGGRPAEGAGPGAQPEWFYKGDGDCVVACGQP
                     LPSPDFALDGGEEPEIVGLYVIAQDGTPQRLGFAIGNEFSDHVTERRNYLYLAHSKLR
                     CCAIGPELRTGTPPAHMEGASRIRRGGDVVWERPFVTGEANMCHSLANLEYHHFKYPA
                     HRRPGDVHLHFFGTATLSFVDGFHAQVGDVFEIALPELGAPLANPLARTDGGFTFGGV
                     RAL"
     gene            2888..3856
                     /locus_tag="VIV63_14345"
     CDS             2888..3856
                     /locus_tag="VIV63_14345"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6125827.1"
                     /translation="MTTTTPWFIRARLKTRQLMLLTAICDEGNIHRAAEVLNMSQPAA
                     SKLLKDLEDMLGVSLFERQPRGMRPNWYGETMIRHARIALSSLREAGQEIDALKAGLS
                     GHVSLGAITGPALSLIPQAIMIVARENPKLRVELHVESSNVLIEQLAQGKIDIMIGRL
                     FERQEKSGLRYERLAEEPVSAVVRPGHPLLNVAGLGLAAVEHKPWIVPPAGSVLRHRF
                     DLMFSEAGHAPPTQLIETASLLFVTKMLQQTDFIAVVPTEVARYYATYGMVAMLPMQL
                     SCTMDAFGIITRTDWLLSPAATVLLRALKTSAISTYGCTLDLKNLS"
BASE COUNT          700 a         1283 c         1324 g          738 t
ORIGIN      
        1 cgggcggtgt ggcggcagtc tccgctcctg tcttgcggcc caccatcgcc gcgaccagcg
       61 cggcgtggtc gagctccgct gccgggtatt cacccaccag ccggccattg cgcagcacgg
      121 tgatgcgatc ggacaccgcg tagacctggt cgaggaaatg cgtgacgaac acgatcgcca
      181 tgcccgattc acgcaggcgc cgcatggccg cgaacagttt ttcgacttcg tcgctgtcga
      241 ggctcgacgt gggctcgtcg aggatcagca cgcgcgccga gatgtcgatg gcgcgcgcga
      301 tggcgaccat ctgctgcaag gcgaccgaat aggaggacaa ctcgcgccgc acgtcgatgt
      361 cgatgtcgag gccggctaac aaggttcgcg cgcgggcgtt cagcgtgcgc cagtcgatgc
      421 ccccgccgcg agtccagcgg cgcagcggct ggcgtccggc gaaaatgttt tcggccaccg
      481 agaggttgcc gcagaggttc acctcctggt acacggtgct gatgccgagg cgctgcgctt
      541 cgcggttgga agtgggcgcg atgcgctggc catccagcac gatcgacccg cgctgcagga
      601 tctcggcgcc ggtcaacacc ttgatcagtg tcgacttgcc ggcgccgttc tggcccatga
      661 gcgcatggat ctcgccggcg cgcagcgtga agtccacgcc gtcgagcgcc ggcttgccgg
      721 cgtaggcgtg aacgatgcca cgcgcctgca ggagatcagc caccgcgctc tcagtatttg
      781 cgcgtcggaa gtacctgtgc cgccgtttcc ctggggaaca ccgactcctc ggtgacgatg
      841 cgcttcggaa tctccttgcc ggccaccacg tccttgaccg ccgccatgag ctgcggacct
      901 aacaacgggc tgcattccac cgtgacgttg agcttgccgg cgatcatcgc ctcgaacgcg
      961 cctttcacgc cgtcgatgga cacgatgatg atgtcggtgc cgggcttgag ccccgcttcc
     1021 tcgatggcct ggatggcgcc gatggccatg tcgtcgttgt gcgcgaacag cacgttgatc
     1081 ttcttgcctt ccgccttgag gaaagcctcc atgacttcct tgcccttggc gcgcgtgaag
     1141 tcaccggtct gcgagcggat gatcttgaag cgcggttcgg caccgacgat ttcggcgaaa
     1201 cccttcttgc gatcgttggc gggcgcggag cccacggtgc cctgcaactc gacgatgtgg
     1261 atgtcgccct gcgcggcttt gccttcaccg gtcgcgtagt actcggtcag ccatcggccg
     1321 gccttgcggc cttcgtcgac gaagtccgag ccgatcagcg acagatacaa ggatgcatcg
     1381 gaaacctcga cggcgcggtc ggtcaggatg acggggatgc cggcggtctt ggcctcgcgc
     1441 agcacggtgt cccagccggt ttcgaccacg ggcgagaacg cgatgacgtc gacgcgctgg
     1501 gcgatgaacg agcgcagcgc cttgatctgg ttttcctgct tctgttgcgc atcggagaag
     1561 cgcagcgtga tgccggcttc cggcgcggcg ctctggatag acgtggtgtt ggcggtacgc
     1621 cattcacttt cggcgccgat ctggctgaac ccgagcacga tggccttgct gccgtccgcg
     1681 gcggccttct tgtcgccgcc cgagcaggcc acgagtagcg ccgcggccgc ggtgaccagc
     1741 gtcatctgaa tgaatctctt catcgttccc cctcaagtag ttggtggtct agagtgcgcg
     1801 aacgccgcca aaggtgaaac caccgtccgt gcgcgccagc ggattggcca gtggcgcgcc
     1861 aagttccggc agggcgatct cgaagacatc gcccacctgg gcgtggaatc catccacgaa
     1921 gctcagcgtg gccgtgccga agaaatgcag gtgcacatca cccggacgcc ggtgcgccgg
     1981 gtatttgaaa tggtggtact cgaggttcgc gagcgaatgg cacatgttgg cttcgccggt
     2041 gacgaacggg cgttcccaca cgacgtcgcc gccgcggcga atgcggctcg cgccttccat
     2101 gtgcgccggc ggcgtgccgg tgcgcagttc cggcccgatg gcgcagcagc gcagcttgga
     2161 gtgcgcgagg tagaggtaat tccggcgctc ggtgacgtga tcgctgaact cgttgccgat
     2221 ggcgaacccc aggcgttgcg gcgttccatc ctgcgcgatg acatacaggc cgacgatctc
     2281 cggctcttcg ccgccatcga gcgcgaagtc gggcgagggc aaaggctgcc cgcacgcgac
     2341 cacgcaatcg ccatcgcctt tgtagaacca ctcgggctgc gcgccaggtc cggcgccttc
     2401 ggcgggacgg ccgccttcga ctccccactt gaacatctgc atggagtcgg acagcgtctc
     2461 cgcggccagc ttcttgtgca tctggtcgcg cgcggacgcg ctgccgtagt gagtgagccc
     2521 ggtacccgag acgaggcatc gcgccggatc cggatgggtc agcggcgcga gcacccggcg
     2581 gccgtccaac aacgcgaggt acggaatgga cgggggcgcg gcctggcgcg aggcgaacgc
     2641 gtcgagcggc tcggccgcgg acgatgccgc ctgcacgagc tcagacgtgg attgaaatga
     2701 gccgacactg ccgacggacg cgccgtcgtc agtaacccag cccacccgcg gttgaccgct
     2761 ctcgtctaag aattggatga gtctcaaggg aggaatcatg ggattcgtat tgatatccaa
     2821 caagcccgat attgctacgg agctataccc atagttatat agttggctcg agacggggga
     2881 gaagacaatg acgacgacaa cgccgtggtt cattcgcgcg cgcctcaaga cgcgccagct
     2941 catgttgctc accgcgatct gcgatgaagg caacattcat cgcgccgccg aagtgctcaa
     3001 catgtcgcag cccgcggcat cgaagcttct caaggacctc gaggacatgc tcggcgtctc
     3061 gctgttcgag cggcaaccgc gcggcatgcg gcccaactgg tacggcgaaa ccatgatccg
     3121 tcacgcgcgc atcgcgctgt cgtcgttgcg cgaggcgggc caggagatcg acgcgctcaa
     3181 ggccgggttg tcgggacacg tgagtctcgg cgccatcacc gggcccgcgc tcagcctgat
     3241 cccgcaggcg atcatgatcg tcgcgcgcga gaatccgaag ctgcgcgtcg agctgcacgt
     3301 cgagtcgagc aacgtgctca tcgagcagct cgcgcagggc aagatcgaca tcatgatcgg
     3361 ccgcctgttc gagcgtcagg aaaaatccgg tttgcgttac gaacgtctcg ccgaggagcc
     3421 ggtgtcggcg gtagttcggc cgggccatcc gctgctcaac gtcgccgggc tcggcctcgc
     3481 ggccgtcgag cacaagccgt ggatcgttcc gcccgcggga agcgtgttgc gtcatcgctt
     3541 cgatctcatg ttcagcgaag cggggcatgc gccacccacg caactcatcg agacggcgtc
     3601 gctgctgttc gtgaccaaga tgttgcagca gacggatttc atcgcggtgg tgcccaccga
     3661 ggtcgcgcgt tattacgcga cttacggcat ggtcgcgatg ctgccgatgc aactttcctg
     3721 cacgatggat gcgttcggca tcatcacccg cacggactgg ttgctgtcgc ccgcggccac
     3781 ggtgctgttg cgcgcgctga agacctcggc catcagtact tatggttgca cgctggacct
     3841 gaagaacctt tcttgatcgc gtgaaaccat caggcatgcc aaagcatgta tggttgggcc
     3901 tgagaaaact attactcgcg tatgttttcg ccttgtacgc tgatgcgcga tggacgttcc
     3961 caaaaagcgc atacgacgca gtcaggcctg gttcggaagg gaagggaaag acggattcct
     4021 gcaccggagc tggatcaaga atcag
//
LOCUS       DATDEU010000599         4817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2015788, whole genome shotgun sequence.
ACCESSION   DATDEU010000599 DATDEU010000000
VERSION     DATDEU010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015788"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..2280
                     /locus_tag="VIV63_14350"
     CDS             70..2280
                     /locus_tag="VIV63_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125828.1"
                     /translation="MKILPALLIACGALAVAHARPVVIQESARIGNPDPTTYPYFASD
                     VAIDGDDAIATLERYIEPGPDSDPRDIKHDVAVYLFRRTGTGWAPVRQLVVHHHGVFN
                     SFISGLAMRNGIAALALNPLYVFERRDGDWVSAPVTGVNQEMPGDSINIDGSRILFGG
                     SSGTWLGTLYEKNSTGVWGPTSLMWGDYRGGDDEFTGGPVDISGGRAVVMSPSNEEEF
                     PVAAPAVTVFRDWGAPDRFLRQSDIRSTPAAPLGYEVAIRGDEIFIAGNNASGTRVFR
                     PGESDQWDEFDKLQPLDSFMGGGVTTVIEKNHLFVMQRNWNAERDAYVINVFRKPVSG
                     PYEHVATLVSSDGSSLNNFGISSRRVIANCGNEACYFELPASLNQPAPVQHTFAAATP
                     TGWATSAGSAFSIVQRGISRVLRQSDVLSPATHSAVLSASDWTNQSVQADVRPLAFAA
                     GDCWQGLATRYRDAGNYYFVSVTSSSVSLRRVVGGVLTTVSTIRAAIPLNRPYRLRLE
                     SIGTRHRVYLNGAPLLDADDSALASGRAALLTHRSSAEFDNVVASPTPATILYSHDFT
                     TSGGPTDLWAFTGAGLWRTVSRGSNFVFAQSSVAGVTRAVTGVPTDDQSVDVRARPTT
                     FAGTGSGERWFGVMARYVDEANYYYLSLRSTNTVSLRKLTNGAITELGTYALPVTLGA
                     WYQLRLEAVGSQIRGYVNGFLVAEATDTTHARGAAGPITNRAAVDFDDYRAIQP"
     gene            2404..>4817
                     /locus_tag="VIV63_14355"
     CDS             2404..>4817
                     /locus_tag="VIV63_14355"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY6125829.1"
                     /translation="MDKAYSPGAIEARIYERWESSGAFAPQGNGTSYCIMLPPPNVTG
                     TLHMGHAFQHTLMDTLTRWHRMRGDATLWQPGTDHAGIATQMVVERQLGAEGKHRLDL
                     GREAFIERVWKWKGESGSTITRQMRRLGTSVDWSRDKFTMDPDLSRAVTEVFVRLHDE
                     GLIYRGKRLVNWDPVLLTAVSDLEVLSEEENGSLWHIRYPVVGGDGHVVVATTRPETL
                     LGDTAVAVNPDDERYRALLGKQLTLPLTGRTIPVIADSYVDAAFGSGAVKITPAHDFN
                     DYEMGQRHSLPQINIFTPDAKLNENAPERFRGLDRFVARKQIVAELEAAGLIEKIEPH
                     KLKVPRGDRTNAVIEPYLTDQWYVKIAPLAEPALKAVEDGRIRFVPDNWSKTYYEWMR
                     NIKDWCVSRQLWWGHRIPAWYDEAGKIYVGRDEAEVRKKHSLAPDLSLRQDEDVLDTW
                     FSSALWPFSTLGWPDKTPSLNQFYPTSVLVTGFDIIFFWVARMIMMGLKFQGDVPFRD
                     VYITGLIRDENGDKMSKAKGNIIDPLDVIDGISLADLLAKRTSGLMQPHLKEKIEKAT
                     RKQFPDGITAFGTDALRFTFASLAGPSRDINFDFGRVGGYRNFCNKLWNGARFVLMML
                     EGDAESSTTATGADLTGEAQLSVADRWIVSRFAATLSQVDSALNEYRFDFAATALYEF
                     TWYEFCDWYLELTKPILQGETTTEAQKRGTRRTLVTVLEALLRALHPMMPFITEEIWQ
                     RVYPLAAPLLAAQETRANQKSFDTVMLAGYPQASGFAADGEADREVAWMKQFILAVRQ
                     IRGEMD"
BASE COUNT          868 a         1615 c         1497 g          837 t
ORIGIN      
        1 catccgctgc gccgcaactc tggtctgatg cgtacgcggc catgacggcg cgcggtctaa
       61 ctacgcctca tgaaaattct gcctgctctc ctgatcgcct gtggtgcgct ggccgtggca
      121 cacgcgcgac cggtggtcat ccaggagtcg gcgcgcatcg gcaaccccga ccccaccacc
      181 tatccctact tcgcatccga cgtcgccatc gacggcgatg acgcgatcgc gacgctcgaa
      241 cgctacatcg agccgggtcc cgacagcgat ccacgcgaca tcaagcatga cgtcgcggtc
      301 tacctgttcc ggcgcacggg caccggctgg gcgccggtgc gccagctcgt ggtccaccat
      361 cacggcgtgt tcaacagttt catctcggga ctcgcgatgc gtaacggcat cgccgcgctc
      421 gcgctcaatc cgctgtacgt gtttgaacgg cgcgatggcg actgggtctc cgcgccggtc
      481 acgggtgtca accaggagat gccgggcgac tcgatcaaca tcgatggctc gcgcatcctg
      541 ttcggcgggt cgagcggcac gtggctcggc acgctctacg agaaaaattc cacgggggtc
      601 tggggtccca cttcgctcat gtggggcgac taccgcggcg gtgacgacga gttcaccggc
      661 ggcccggtcg atatctccgg cgggcgcgcc gtggtgatga gcccgagcaa tgaagaagaa
      721 tttccggtcg cagcaccggc tgtcaccgtc ttccgcgact ggggcgcgcc cgatcgtttc
      781 ctgcgtcagt ccgacatccg cagcaccccc gccgcaccgc tcggctacga ggtcgcgatt
      841 cgcggcgatg agatcttcat cgcgggcaac aacgcctcgg gcacgcgcgt cttccggccg
      901 ggagaaagcg accagtggga cgagttcgac aagttgcagc cgctcgacag cttcatgggc
      961 ggtggcgtaa ccaccgtcat cgagaagaat catctcttcg tcatgcaacg caactggaac
     1021 gcggagcgcg acgcctacgt catcaacgta ttccgcaaac ccgtgagtgg tccgtatgag
     1081 cacgtggcca cgctggtttc gagtgacgga tcctcgctga acaacttcgg catcagcagc
     1141 cggcgcgtga tcgcgaactg tgggaacgag gcgtgttact tcgagttgcc ggcgagcctg
     1201 aaccagcccg cgccggttca gcacaccttc gccgcagcga cgcccacggg atgggcgacg
     1261 agtgccggca gcgcattctc gatcgtccag cgcggcatca gccgcgtgtt gcggcagtcc
     1321 gacgtgctga gtcccgcgac acattccgcg gttctcagtg cctcggactg gaccaatcaa
     1381 tcggtgcagg ccgatgtgcg tccgctcgcc ttcgccgccg gcgactgctg gcagggactc
     1441 gcgacgcgtt accgcgatgc cggcaactac tatttcgtgt cggtgaccag ctcgagcgtg
     1501 tccttgcgtc gcgtcgtggg tggagtgctc accaccgtgt cgacgatacg ggctgccatc
     1561 ccactgaacc gcccataccg cctgcggctc gagtcgatcg gtacgcgtca ccgcgtgtac
     1621 ctcaatggcg cgcccctgct cgacgccgac gacagcgcgc tcgcgagcgg ccgcgccgcg
     1681 ctgctcacgc accggtcttc ggcagaattc gacaacgtag tcgcgagccc gacgcccgcg
     1741 acgatcctgt actcccacga cttcacgacc tctggcgggc cgacggattt gtgggcgttc
     1801 accggcgcgg gactctggcg cacagtttcg agaggatcga acttcgtgtt tgcccagagt
     1861 tccgtggccg gcgttacccg cgccgtgaca ggcgttccca ccgacgatca gtccgtggat
     1921 gtccgggcgc ggcccactac gttcgcgggc accggcagcg gcgagcgctg gttcggcgtc
     1981 atggcgcgtt acgtggacga agcgaactac tactacctgt cattgcgcag cacgaacacg
     2041 gtatcgctgc gcaagctcac gaacggggcg atcacggagc tcgggactta cgcgctgccg
     2101 gtcacgctgg gggcgtggta ccagctgcgg ctcgaagccg tcggctcgca gatccgcgga
     2161 tacgtcaatg gcttcctggt ggccgaagcc accgatacca cgcatgcgcg cggggccgcg
     2221 ggccccatca ccaatcgcgc cgccgtggat ttcgacgact atcgagccat ccagccctaa
     2281 aaaaggggac agatctattt ttcgatgatc gcccgcgcct gacttgcggc cgatcgtcga
     2341 aaaatagatc tgtccccttt ttttggccta cccctttttc gcccccgtat aatccgcgcc
     2401 ctcatggata aagcctattc accgggcgca atcgaagccc gcatctacga acgttgggaa
     2461 tcctcgggcg ctttcgcgcc gcagggcaac ggcacgtctt actgcatcat gctgccgccg
     2521 ccgaacgtca ccggcacgct gcacatgggc cacgcgttcc agcacacgct catggatacg
     2581 ctgacgcgct ggcatcgcat gcgcggcgac gcgacgctct ggcagccggg cacggatcac
     2641 gccggcatcg ccacgcagat ggtggtcgag cgccagctcg gcgcggaagg caaacaccgg
     2701 ctcgacctcg ggcgcgaggc gttcatcgaa cgcgtctgga agtggaaagg cgaatccggc
     2761 agcaccatca cgcggcagat gcgccgcctc ggcacgtcgg tggactggtc gcgtgacaag
     2821 ttcacgatgg accccgacct ctcgcgcgcc gtcaccgaag tgttcgtgcg gctgcatgac
     2881 gagggcctca tctaccgcgg caagcgcctg gtcaactggg atccggtgct gctgaccgcg
     2941 gtctccgatc tcgaagtgtt gtccgaggag gaaaacggct cgctctggca catcaggtat
     3001 cccgtggtgg gtggtgacgg ccatgtcgtc gtggccacga cccgccccga gaccttgtta
     3061 ggtgacaccg ccgtggccgt gaacccggac gacgagcgtt atcgcgcgtt gctcggcaaa
     3121 caactgaccc tgccgctgac cggccgcacc attccggtga tcgccgacag ctacgtcgac
     3181 gccgcattcg gctcgggcgc cgtgaagatc acgcccgcgc acgacttcaa cgactacgag
     3241 atgggccagc ggcactcgtt gccgcagatc aacatcttca cgcccgatgc gaagctcaac
     3301 gaaaacgcgc ccgagcgctt ccgcgggctc gaccgtttcg tcgcgcgcaa acagatcgtc
     3361 gccgaactcg aggcagccgg cctcatcgag aagatagagc ctcacaagct caaggtgccg
     3421 cgcggcgatc gaacgaacgc ggtcatcgag ccctacctca ccgaccagtg gtacgtgaag
     3481 atcgcgccgc tggccgaacc cgcgctgaaa gcggtcgaag acggccgcat ccgcttcgta
     3541 cccgacaact ggtcgaagac ctattacgag tggatgcgca acatcaagga ttggtgcgtg
     3601 agccgccagc tctggtgggg ccatcgcatt ccggcgtggt acgacgaagc cggcaagatc
     3661 tacgtgggcc gcgatgaagc cgaggtgcgc aagaaacact cgctcgcgcc cgatctctca
     3721 ctgcgccagg acgaggatgt gctcgacacc tggttctcgt cggcgttgtg gccgttctcc
     3781 acgctcggct ggccggacaa gacgccctcg ctgaaccagt tttacccgac cagcgtgctg
     3841 gtcacgggct tcgacatcat cttcttctgg gtcgcccgca tgatcatgat gggcctgaaa
     3901 ttccagggcg acgtgccgtt cagggacgtc tacatcaccg gcctcatccg cgatgagaac
     3961 ggcgacaaga tgtcgaaagc caagggcaac atcatcgacc cgctggatgt catcgacggc
     4021 atcagcctcg cggacctgct cgcgaagcga acgagcggcc tcatgcagcc gcatctgaaa
     4081 gaaaagatcg aaaaggccac gcgcaagcaa tttcccgacg gcatcaccgc gttcggtacc
     4141 gacgccctgc gcttcacgtt cgcgtccctg gccggtccgt cgcgtgacat caacttcgac
     4201 ttcggccgcg tgggcggcta tcgcaacttc tgcaacaagc tgtggaatgg cgcgcgcttc
     4261 gtgctgatga tgctggaggg cgacgcggaa tcttctacca ccgcgaccgg cgccgacctc
     4321 acgggcgaag cccagctctc ggtcgccgat cgctggatcg tctcgcgctt cgcggccacg
     4381 ctgtcgcagg tagatagtgc gctcaacgag taccgcttcg acttcgccgc cacggcgctg
     4441 tatgaattca cgtggtacga gttctgcgac tggtacctgg agctcaccaa gcccatcctg
     4501 cagggcgaga ccaccaccga ggcgcagaag cgcggcacgc ggcgcacgct ggtgacggtg
     4561 ctcgaagcgc tgttgcgcgc gttgcatccg atgatgccgt tcatcaccga ggaaatctgg
     4621 cagcgcgtgt atccgctcgc cgcgccattg ctggcggcgc aggaaacccg tgccaaccag
     4681 aagagtttcg acactgtgat gctcgccggc tatccccagg ccagcggttt cgccgccgat
     4741 ggcgaagcgg accgcgaggt cgcctggatg aagcaattca tcctcgcggt gcggcagatc
     4801 cgtggcgaga tggacat
//
LOCUS       DATDEU010000600         4365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1578025, whole genome shotgun sequence.
ACCESSION   DATDEU010000600 DATDEU010000000
VERSION     DATDEU010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578025"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VIV63_14360"
     CDS             complement(<1..191)
                     /locus_tag="VIV63_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125830.1"
                     /translation="MTLPLKFALAVALTAVAACAALYFFREQPIEAPPSKGEVMLAPT
                     SSVEPSVGPPSSEVSRADVP"
     gene            240..1709
                     /locus_tag="VIV63_14365"
     CDS             240..1709
                     /locus_tag="VIV63_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6125831.1"
                     /translation="MRIPRRDLLKWGAWAAAAGLLPATAARAAEARREPVTLAPGKPK
                     TVLVIGAGIAGLVAAYELRKLGHQVTVLEAQRRPGGRVYTLRDPFSDGLYVEVGAARI
                     PDNHDLTLRYVREFNLPMTPFYPATGSRMYSIGGKRIRADGSGEWMAGEAPLDLKPDE
                     KKLGQWALLEKYVGGALAELGDPYKIDYASAAARKFDSVTTAQYLRQQGASEAANDLM
                     SWPWATAKDDRYSFLWTLREIAYESQEKTRTKIVGGNDKLPRAFADTMKESIHYGSPV
                     VRIEQDDRRVRAIVRRAGGTETFEADRLLCTVPFPALRDVDIAPALSAAKRRVINELA
                     YDTIVRATLQSRTRYWEKDGFNGFGHSDTPQQIWHFTHDQPGPRGLLVSFICGATGGR
                     VGDMEPEARERFLVAEMERAHPGLTDHYEGAFVHVWHKHPWAKGALALPSPGQMTGMC
                     VGAEKPEGRIHFAGEHLSHFSGWIQGALESGLRAVRELA"
     gene            complement(1722..2960)
                     /locus_tag="VIV63_14370"
     CDS             complement(1722..2960)
                     /locus_tag="VIV63_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6125832.1"
                     /translation="MKRLAFASIAGTALEYYDFAVYNTLAALVFNKLFFPSVDPLTGT
                     LLAFATYWVGYLSRPFGGILFGNLGDRRGRRYVLVMTLLLMGACTTLIGLLPTYETVG
                     VLAPILLVTLRFVQGMALGGEWAGAVLLTVEHGTARQRGRNAAFAQMGPSTGVLIATG
                     TVALISWLMSEEQLLDWGWRVPLLASVLLVAFGLWLRLGVSETPEFIALKDQQSRAPV
                     SEVFSGHWRRLLVAGGSRFGPDVMYSLISAFCVSYVTTILGLPRTLATGALAIGAFFN
                     VLLIFAAGTLSDRFGRRAIYGVGVGAAALWLTALFPMLNSGSEFLVVAAFVSGLAVHA
                     FMYGPQGAFIAEQFPVRVRYVGASVAYTFAGVFAGGLAPLAFTRIYQASHETRYIVVY
                     VLVALTITGIALRLSRSADR"
     gene            complement(2975..>4365)
                     /locus_tag="VIV63_14375"
     CDS             complement(2975..>4365)
                     /locus_tag="VIV63_14375"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY6125833.1"
                     /translation="GQLIANAPHMPVHLGSMGESVQEILRRRGADMQRGDAFALNAPY
                     AGGTHLPDVTVVMPVFLAGEKPEFFVAARGHHADIGGITPGSMPSDSKNIDEEGVLID
                     SVRIVDRGAFLEDAVRELLTSGAWPVRNVTQNLADLRAQIAACQKGIEELNAMCAHYG
                     LEVVQAYMQHVQDNAEAAVRKVIGVLKDGEFSYDMDNGAVIRVAIRIDPARREATIDF
                     TGTSAQQPNNFNAPLPVTRAAVLYVFRTLVDDEIPMNAGCMKPLHLIVPEACMLAPRY
                     PAAVVAGNVETSQVVTDCLYGALGVLAASQGTMNNFTFGNDEHQYYETIAGGSGAGPH
                     FDGTDAVQTHMTNSRLTDPEVLEWRFPVRLESFRIRRGSGGAGAHRGGDGVERRLRFL
                     EPMTAVMLANHRRVAPFGLAGGEAAATGRNRVERAAGVTEHFDAAFSTQVVRDDVIVI
                     ETPGGGGFGRKS"
BASE COUNT          797 a         1427 c         1441 g          700 t
ORIGIN      
        1 ggaacgtccg cgcgggaaac ctcgctggac ggaggcccca ctgacggctc gactgaagaa
       61 gtcggcgcga gcatcacctc gcctttggag ggaggcgctt cgatcggctg ttcgcggaag
      121 aaataaagag cagcacatgc cgcgacggcg gtcagcgcta cggcgagagc aaacttcaag
      181 ggcagggtca tggcggggat agtgtcgccc gatgagggtg tgatagaaag ccctaacaaa
      241 tgcgcatacc tcgacgtgat ctcctgaagt ggggcgcctg ggccgcggcc gcgggcctgc
      301 tgccggcaac cgcggcgcgc gcggcggaag cccgccgcga gcccgtcacg ctcgctcccg
      361 gcaaaccaaa aaccgttctc gtcattggcg ccggaattgc cggcctggtc gcggcctacg
      421 agttgcgcaa gcttggccac caggtgacgg tgctcgaggc gcaacgccgt ccgggtggcc
      481 gcgtgtatac gttgcgcgat ccgttctcgg atggcctgta tgtcgaagtg ggcgccgcgc
      541 gcattcccga caatcacgat ctcacgctgc gttacgtgcg cgagttcaat ctgccgatga
      601 cgccctttta tcccgcgacc ggctcgcgca tgtactccat cggcggcaag cgcattcgcg
      661 cggatggcag tggtgagtgg atggcgggcg aggcgccgct cgatctgaag cccgatgaaa
      721 agaagctggg gcagtgggca ttgctcgaaa aatacgtcgg cggtgcgctg gcggagctcg
      781 gcgatcctta caagatcgac tacgcctcgg ccgccgccag gaaattcgat tccgtgacca
      841 ccgcgcaata cctgcgtcag cagggtgcct ccgaggccgc gaacgatctc atgagctggc
      901 cgtgggccac cgcgaaagac gaccgatact cgtttttgtg gaccctgcgc gagatcgcct
      961 acgagtccca ggaaaagacg cgcacgaaga tcgtcggcgg caacgacaaa ctaccgcgtg
     1021 ccttcgccga caccatgaaa gagtccattc attacggctc gcccgtggtg cgcatcgagc
     1081 aggacgaccg gcgggtccgc gccatcgtca ggcgcgccgg cggcaccgaa accttcgaag
     1141 ccgatcgcct gctgtgcacg gtcccgtttc cggcgttacg cgacgtggat atcgcgccgg
     1201 ccctgtcggc cgcgaagcgg cgcgtcatca acgagcttgc ctacgacacc atcgtgcgcg
     1261 ccacgctgca gagccgtacg cgatactggg agaaggacgg gttcaacggc ttcggccatt
     1321 cggacacgcc gcagcagatc tggcatttca cgcatgacca gccgggtccg cgtggtttgc
     1381 tggtgtcgtt catctgcggc gcgaccggcg gacgtgtcgg cgacatggag cccgaggcgc
     1441 gcgagcggtt cctggtcgcg gagatggaac gcgcgcatcc gggactcacg gaccactacg
     1501 aaggcgcgtt cgtgcacgtg tggcacaaac atccgtgggc caagggcgcg ctcgcgttgc
     1561 catcgcccgg ccagatgacc ggcatgtgcg tcggggcgga gaagcccgag gggcgcatcc
     1621 acttcgcggg cgaacatctc tcgcatttct cgggctggat acagggcgcg ctggagtcgg
     1681 gcctgcgcgc ggtgcgggaa ctcgcctagt gcgcgcggcc cctatctatc cgcgcttcgg
     1741 gacaaccgca gcgctatgcc ggtgatcgtc aacgccacga gcacgtacac gacgatgtac
     1801 cgcgtttcgt ggctcgcctg gtagattctg gtgaacgcca gcggcgcgag tccaccggca
     1861 aacacgccgg cgaaggtgta tgccaccgaa gcccccacgt atcgaacgcg caccgggaac
     1921 tgctcggcga tgaacgcccc ctgcggcccg tacatgaatg catgcacggc caggccgctg
     1981 acgaacgccg ccacgaccag gaattccgat cccgagttca acatcggaaa cagcgcggtc
     2041 agccacagag ccgccgcacc cacgccgaca ccgtagatcg cgcgacggcc gaagcgatcc
     2101 gagagggtgc ccgcggcgaa aatgagcaac acgttgaaaa acgcgccgat cgccagcgcg
     2161 cccgtggcca gcgtgcgtgg caggccgaga atcgtcgtga cgtagctgac gcagaatgcg
     2221 ctgatcagcg aatacatcac gtctggcccg aagcgcgagc ctcccgccac caacaggcgc
     2281 cgccagtggc cgctgaatac ctcgctgaca ggcgcgcgcg actgctggtc cttcaacgcg
     2341 atgaactccg gtgtctcgga gacgcccagg cgtaaccaca gaccaaaggc caccaacagc
     2401 acactcgcga gcagcggaac gcgccatccc caatcgagca gttgttcttc cgacatcagc
     2461 caggagatga gcgccaccgt gccggtggcg atgagcaccc cggtggaggg tcccatttgc
     2521 gcgaaggcgg cgttgcggcc acgttgccgg gccgtgccgt gctcgaccgt gagcaacacg
     2581 gcgccagccc actcgccacc gagcgccata ccctgcacga atctcagcgt caccagcagg
     2641 atgggagcga gcacgccaac cgtttcataa gtgggcagca ggccgatcaa tgtcgtgcag
     2701 gcgcccatga gcagcagcgt catcacgagc acgtagcggc ggccgcggcg atccccgaga
     2761 tttccgaaga ggattccgcc gaatggccgg gagagatagc ccacccagta ggtggcgaaa
     2821 gccagcaacg tgcccgtcag gggatcgacc gacggaaaga acagtttgtt gaacaccagc
     2881 gccgcgagcg tgttgtacac cgcgaagtcg tagtactcga gcgccgtgcc cgcgatgctc
     2941 gcgaacgcca ggcgtttcat gtccggggct ttgctcaaga cttcctgccg aagccgccgc
     3001 cgcccggcgt ttcgatgacg atgacgtcgt cgcgcaccac ctgcgtcgag aacgccgcgt
     3061 cgaaatgttc cgtgactccc gcggcgcgtt cgacacggtt gcgtccggtt gccgccgctt
     3121 ctccgccggc gaggccgaac ggcgcgacac gccggtggtt agccagcatg acggccgtca
     3181 tgggctcgag gaatcgcagg cgccgctcga caccatctcc gccgcgatgc gcgccggcgc
     3241 cgcccgagcc gcggcgaatt cgaaacgact ccagccggac cgggaagcgc cactcgagca
     3301 cttccggatc tgtcaggcgg gagttggtca tgtgggtctg cacggcatcg gtaccgtcga
     3361 agtgaggccc cgcgcccgag ccaccggcga tggtttcgta atattggtgc tcgtcgttgc
     3421 cgaacgtgaa gttgttcatc gtgccctggc tggccgcgag cacgccgagc gcgccataca
     3481 ggcagtctgt cacgacctgc gacgtctcga cattgccggc gacgaccgcg gctggataac
     3541 gcggcgccag catgcaggct tcgggcacga tgagatgcag cggcttcatg caaccggcat
     3601 tcatcgggat ttcatcgtcg accagcgtgc ggaacacata cagcaccgcg gcgcgtgtca
     3661 ccggcagcgg tgcattgaag ttgttgggct gttgcgcgct ggtgcccgtg aagtcgatgg
     3721 tggcttcgcg gcgcgcaggg tcgatgcgta tcgccacgcg gatcacggcg ccgttgtcca
     3781 tgtcgtaact gaattcgccg tccttgagca cgcctatgac tttgcgcacc gccgcctcgg
     3841 cgttgtcttg cacgtgctgc atgtacgcct ggaccacctc gaggccgtag tgcgcgcaca
     3901 tggcgttgag ctcttcgatg cctttctggc aggccgcgat ctgcgcgcgc aggtcggcga
     3961 gattctgcgt gacgttgcgc acgggccagg cgcctgacgt caggagttcg cgcaccgcgt
     4021 cttcgagaaa cgcgccgcga tcgacgatgc gcacgctgtc gatgagcacg ccttcctcgt
     4081 cgatgttctt cgagtccgac ggcatcgatc ccggcgtgat gccgccgatg tcggcgtgat
     4141 ggccgcgggc cgcgacgaag aactcgggct tttcaccggc gaggaacacc ggcatgacca
     4201 ccgtgacgtc gggcaggtga gtgccgccgg cgtagggagc gttcagcgcg aacgcatcgc
     4261 cacgctgcat gtcggcgccg cgccggcgca ggatttcctg cacggattca cccatagatc
     4321 ccaggtgcac gggcatgtgc ggtgcgttgg cgatgagctg cccct
//
LOCUS       DATDEU010000601         8220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_558926, whole genome shotgun sequence.
ACCESSION   DATDEU010000601 DATDEU010000000
VERSION     DATDEU010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558926"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3585)
                     /locus_tag="VIV63_14380"
     CDS             complement(<1..3585)
                     /locus_tag="VIV63_14380"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125834.1"
                     /translation="MNAGMFRTRAAFAFLCLLLPLSASAVITRTELAGNPLAQFPFFE
                     YVRAFNVNAPVNIAIDPSRFPAIAGDTCDIHVVAHKSPATWASTPALVDVTPGGAITQ
                     AFAGANIQANTVVVAAASTLSATAGDGLGVGYDVVLDCDQNGSLSDGDYIDGLSDAAG
                     LYMVHDTTAPGTHAVTQENTMIQFMVGGSFGIPVANLSERIYFPSDIAGMGQLPLVVI
                     GHGNGHQHTWYDHIGNHLASYGYIVISIDNDTGPGPISASITQLGHTDAFIDQAEAGV
                     LAGGALAGHVDSQRIVWIGHSRGAEGVAIGYDSIFDSTYVPVNFTKKSIRIISSMLPT
                     DFEGTATANPHDANYHLWTASGDDDVNGSAGDQCNWGTDLLELCQTFHLVERATGFRQ
                     STVVQGTGHAWFHAGGGPSVFTGPCSIGETNTHLVQLGYLLPLVKHYVEGNVPALDFL
                     TRQYESFRPIGVPTGDPCIVVSHEYLNGSQVGTFVVDDYQTQTATATSSSGGTVTSDV
                     DNVTEGRLDDNNSTFVSDATDPFNGSTQGGVNDVRTNRNDQSRGVVFDWTGANRFYEW
                     EVTAGHTNFSRFGYLQFRGAQGTRHPNTIAALGDLTFVVTLRDLSGNTSSINIGAFGG
                     GLEEPFQRDGGWHNEMETIRIRLTDFLNNAAPLDLSNVVAVRLSVGPASGSNEGRIVI
                     DDLMLTNDVTPHTLTIVEPTTVRPAYAGTSLVGSRVLVRLIGGSGLDMSAGNLTISVA
                     GVALTPLQIPTPPTAVGGETWVVIAPGPRADGCYDLAVALIAPADVSAAEPQSLCYAD
                     DETHVFDRVLAIDQTNSMNYDGNTGLSSNAKMEAAKAAAKFFVDLSNPADKIGVISFQ
                     RRDQDEDGSIVDPDELAEPQFSFIEAGEGITDQRPAARAAITGINPDADPGFWGNETS
                     PGAGLVEARTMLDAGAVVGHEPNIVLLTDGLENYAPFWTAAGPGGPLRPVFLADDIRV
                     DTVGVGGDADDVLLQDIADATGGEFRNLNEGSGSFFLLSRMADFYKSVDEDVRGEQRF
                     YYAEGFPTPTIPIQDKSWHVATFDVEAGLDWMTVAFHANVDGAYQVRLFAPGSATPVA
                     AAPPVITVRSDAKHVVYRIRTPAAGQWKYIVDVRRRDAEFFAAASALTSLTAKIGPNQ
                     LARRPAGDYLMPLRLWIAERVSVVGANVSGYVRQPDGVKIPVTLVDNGL"
     gene            complement(3582..4211)
                     /locus_tag="VIV63_14385"
     CDS             complement(3582..4211)
                     /locus_tag="VIV63_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125835.1"
                     /translation="MKLSSAALALILLGIPVLGACQTSGTRARETPPTPASIKGILVA
                     QRFTLSTPYVNTWSKARAQVSSGTIVVLEVDPAYLVPTDAAQPVLYAGDVAVQRLNQG
                     HQSGRVIGIVPGNVDLTTAPIWFGTPGLPERVTPESARAERALAEKAGIRPLAAARVA
                     AVTRTPVSADNLLTVLRTTIANLVLQYSPQEKHLADKWRVPEARQRSQR"
     gene            4370..4906
                     /locus_tag="VIV63_14390"
     CDS             4370..4906
                     /locus_tag="VIV63_14390"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEY6125836.1"
                     /translation="MALGATIYTFDIELADSDRAVYQALNLRVAQHPSETPDYLMTRV
                     LAYCLEFSEGIGFSKGLSDPDEPAIFVRDLTGALMVWVDIGLPEPDRLHRASKAAPRV
                     AVYTHKDAAQWAVRIRDAKIHRAEKLEAFAFDRAWLTAVVARLERRMKFSLARSEGEI
                     YLTLGADTLQTVLERPLT"
     gene            5041..5739
                     /locus_tag="VIV63_14395"
     CDS             5041..5739
                     /locus_tag="VIV63_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125837.1"
                     /translation="MAGLLAYVSCLAAMPLAAQVGPIQPVLVLSDGKLETNHDRPSFD
                     EDYSIPVDVYVAADGSVTNVVVTGTSKNFNADAVAADYMRTRKFLPALDHQGQAVEGM
                     AKVNVNMFKRGTKKVVRVTLKPPPMAVETERLRRMMCADFVWEVNRMHEQAGVRDASL
                     ETMPYISAHMYAEGKDMAGEVAEKFWDTWPKTLGKVVERCEKAPEKMYFTEVLVPALD
                     GTMPTRDTVTAAAP"
     gene            5781..6482
                     /locus_tag="VIV63_14400"
     CDS             5781..6482
                     /locus_tag="VIV63_14400"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6125838.1"
                     /translation="MFIHFDADRASDSPFVERVWRCHSEHGGPFLAVASSHWELVVTR
                     LAGTTTVTLHGPETRAREVHCPANGEWFAIRFKAGTFMPRLPVANLLNGQDVNLAPAF
                     RGRFRLEGGAWEIPDFENAETFVARLARRGIITRDPAVEAALGGDELALTRRSAQRHF
                     LHATGMSFTAMKNIERARAATQLLRAGASPTEAAFETGFFDQPHLTRSLRQLIGLTPA
                     RIAREERQLSFLYKT"
     gene            6528..6932
                     /locus_tag="VIV63_14405"
     CDS             6528..6932
                     /locus_tag="VIV63_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6125839.1"
                     /translation="MNKRGRMWWLGWSMSALVVLFLLLDSGMKLAAVRPVLEAGMQIG
                     FPGEAMARKLGLLLLICTALYVWPATSVLGAILVTGFLGGAVATHVRLGNPLFSHVLF
                     GVYVGVLMWGGLFLREERVRALLPVLKPVSAG"
     gene            complement(6936..7703)
                     /locus_tag="VIV63_14410"
     CDS             complement(6936..7703)
                     /locus_tag="VIV63_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125840.1"
                     /translation="MRGQLENSSIQRGLLVESNESNSAPRGDAYAVIQYPYATVAAAF
                     AKAENWCESMILHLNVQYCRANVDEGEPRLSAAVGKNINQPLDDTHQISFSYEVKAST
                     ADFFGVELVAADGPLGTSDYRIALELVALPDDRTFMHIQYSYTQGALARYASGVYFST
                     TGRDKVGFTWIQDEGDAPELVRGTRGALERNTMRYYLAFDAYLRSLNMPEPQRFERSL
                     EIWFADTERFARQLREIKHDDYIAMKRDQYLRQQSPM"
     gene            complement(7962..>8220)
                     /locus_tag="VIV63_14415"
     CDS             complement(7962..>8220)
                     /locus_tag="VIV63_14415"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6125841.1"
                     /translation="EIYVEAGFTPAEALAAATIVPAKLVGQDKRTGSIKPGKVADLVL
                     VEGDPSQRIGDLRNTRVIMLEGKLLDADALRTAAGFSGRPK"
BASE COUNT         1419 a         2599 c         2694 g         1508 t
ORIGIN      
        1 caggccgttg tcgacgagtg tcaccgggat tttcacgccg tcgggctggc gcacataacc
       61 gctgacattc gcgcccacca cggacacgcg ttccgcgatc cacagacgta gcggcatcag
      121 gtagtcgccg gccggacgcc gcgccaactg gttaggcccg atcttcgcgg tcagcgaggt
      181 cagcgccgat gccgccgcga agaattcggc gtcgcgcctg cgaacgtcca cgatgtattt
      241 ccactggccc gcggccggcg tgcggatgcg atagaccacg tgtttggcgt cggagcgcac
      301 ggtgatgacg ggcggcgccg ccgcgactgg cgttgccgaa cccggcgcaa acagccgcac
      361 ctggtacgcg ccatccacat tggcgtggaa cgcgaccgtc atccagtcga ggcccgcttc
      421 gacatcgaac gtggccacat gccacgactt gtcctggatc gggatggtgg gagtcggaaa
      481 tccctccgcg taatagaagc gttgttcgcc gcgaacgtct tcgtcgacgg acttgtagaa
      541 gtccgccatc cggctcagca ggaagaacga tcccgaacct tcgttgagat tgcggaactc
      601 gccgcccgtg gcatcggcaa tgtcctgcag caacacgtcg tccgcgtcgc cgcccacgcc
      661 caccgtatcg acgcggatgt cgtcggccag gaataccgga cgcagcggcc cgcccggtcc
      721 cgctgcggtc cagaacggcg cgtagttctc gagaccgtcg gtgagcagaa cgatgttagg
      781 ttcatggccc accaccgcgc cagcatcgag catggtgcgc gcttccacga ggcctgcgcc
      841 cggcgaggtt tcgttgcccc agaaacctgg atccgcatcc ggattgattc ccgtgatggc
      901 ggcgcgcgcc gcgggccgct ggtccgtgat gccttctccc gcttctatga aactgaattg
      961 gggttccgcg agttcgtccg gatcgacgat ggagccgtcc tcgtcctggt cacgacgctg
     1021 gaagctgatc acaccgatct tgtcggcagg gttcgagaga tccacgaaga acttcgccgc
     1081 cgccttggcg gcttccatct tcgcgttgct cgacagcccc gtgttgccgt cgtagttcat
     1141 gctgttcgtc tggtcgatgg ccagcacgcg gtcgaacacg tgggtttcgt cgtcggcata
     1201 acaaagactc tgcggttcgg ccgcgctcac atcggcgggc gcgatcaatg ccaccgcgag
     1261 gtcataacaa ccatcggcgc gcgggccagg cgcgatgacc acccaggttt cgccaccgac
     1321 cgccgtgggc ggagtcggaa tctgcaacgg agtgagcgcg acaccggcga ccgagatcgt
     1381 gagattgccg gcagacatgt cgagtccgct gccgccgatg agcctgacca gaacgcggct
     1441 gcccacgaga gaggtgccgg cgtacgccgg ccgcaccgtc gtgggctcga cgatcgtcag
     1501 cgtgtgcgga gtcacgtcat tggtgagcat gagatcgtct atgacgatgc ggccttcgtt
     1561 cgagcccgac gccggcccga cactcagtcg aaccgccacg acgttggaca gatcgagcgg
     1621 cgccgcattg ttgagaaagt cggtgagacg gatacggatc gtctccatct cgttgtgcca
     1681 gccgccatcg cgctggaagg gctcctcgag tccaccgccg aacgcgccga tgttgatgga
     1741 gctggtgttc cctgacagat cgcgcagtgt caccacgaag gtgaggtcgc cgagcgccgc
     1801 gatggtgttc ggatgacgcg taccctgggc gccgcgaaac tgcagatagc caaaacgcga
     1861 gaagttcgtg tggcccgcgg tcacttccca ttcgtaaaag cggttcgcgc ccgtccagtc
     1921 gaacaccacg ccgcggctct ggtcgttgcg attggtgcgt acgtcgttga cgccgccctg
     1981 cgtcgagcca ttgaaagggt ccgtcgcgtc cgagacgaat gtggagttgt tgtcatcgag
     2041 ccggccttcg gtcacgttgt cgacgtcgga cgtgacggtg ccgcccgagc tgctcgtggc
     2101 ggtcgcggtc tgcgtctggt agtcgtcgac gacgaaggtg ccgacctgcg agccattcag
     2161 gtattcgtgg ctcacgacga tgcacggatc gccggtgggc acgccgatgg gacggaacga
     2221 ttcgtactgg cgcgtcagga aatcgagcgc ggggacgttg ccttcgacgt aatgtttcac
     2281 cagcggcagc agatagccca actgcactag gtgagtattt gtttcgccga tcgagcacgg
     2341 cccggtgaaa accgacgggc ctccgccggc gtgaaaccac gcgtgccccg ttccctgcac
     2401 cacggtcgat tgccggaagc ccgtcgcgcg ttccacgaga tggaacgtct ggcacagctc
     2461 gagcaggtcc gtaccccagt tgcactggtc accggcggaa ccattcacat cgtcgtcacc
     2521 cgacgccgtc cacaaatggt agttggcgtc atgcggattc gcggtcgccg tgccctcgaa
     2581 atccgtcggc agcatgctgc tgatgatgcg gatgcttttc ttcgtgaagt tcaccggtac
     2641 gtaggtggaa tcgaagatgc tgtcgtagcc gatggccacg ccctcggcgc cgcggctatg
     2701 gcctatccac acgatgcgct gcgaatcgac gtggccggcg agcgcgccac cggcgagcac
     2761 gcccgcctcc gcctgatcga tgaacgcatc ggtgtgcccg agctgcgtga tggacgccga
     2821 aatcgggccc ggccccgtat cgttgtcgat gctgatgacg atgtagccgt acgacgcgag
     2881 gtggttgccg atgtggtcgt accaggtgtg ctggtgcccg ttgccgtggc cgatgaccac
     2941 gagtggcaac tggcccatgc cggcgatgtc cgaagggaag tagatgcgct cgctcaggtt
     3001 cgcgaccgga attccgaaac tgccgccgac catgaactgg atcatggtgt tctcctgggt
     3061 cacggcatgc gtccccggag cggtggtgtc gtgaaccatg tacaggccgg ccgcgtcgct
     3121 caggccgtcg atatagtcgc cgtcgctcaa actaccgttc tgatcgcagt cgagaacgac
     3181 gtcgtacccc acgccgaggc catcacccgc ggtcgcgctg agtgtcgacg ccgccgcgac
     3241 caccaccgtg ttggcctgga tgttggcgcc ggcgaaagcc tgggtgattg cgccaccggg
     3301 tgtcacgtcg acgagcgccg gtgtcgacgc ccacgtggcc gggctcttgt gagccactac
     3361 atggatgtcg caggtgtcgc cggcaatggc gggaaaccgc gaggggtcga tggcgatgtt
     3421 gacgggcgcg ttcacgttga acgcgcgcac atattcgaag aacgggaact gcgcgagggg
     3481 attgccagcg agttccgtac gcgtgatcac cgccgaagcg gatagcggca gcagcaggca
     3541 caggaatgcg aaggccgcgc gcgtgcggaa catgccggcg ttcatcgctg gctccgctgc
     3601 cgggcttccg gcacgcgcca cttgtcggcc aggtgttttt cctgcggcga gtactgcagc
     3661 acgaggttgg cgatggtggt tcgcagcacg gtcagcaggt tgtcagcact gaccggcgtg
     3721 cgtgtgaccg cggcaacgcg cgcggccgcg agcggcctga tgccggcctt ctccgccagc
     3781 gcgcgttcgg cacgcgccga ttcgggcgtc acgcgctcgg gcagacctgg cgtgccaaac
     3841 cagattggcg cggtcgtcag atccacattg ccgggcacga tgccgatgac tcgaccggat
     3901 tgatgaccct ggttcagccg ttgcaccgcg acgtcgcctg cgtacagcac cggctgcgcc
     3961 gcgtcggtcg ggaccaggta cgccggatcg acttcgagca cgacgatggt gcccgacgac
     4021 acctgggcgc gcgccttgct ccacgtgttc acgtagggcg tcgagagcgt gaagcgttgc
     4081 gccacgagga ttcccttgat ggacgcgggg gtcgggggcg tttcacgcgc gcgcgtcccg
     4141 gaggtctggc atgcgccgag caccggaatg ccaagcaaaa tgagcgcgag cgcggcggag
     4201 gagagcttca tgcgtacctc gaaagaactt cttgttctgc catcgaccat acgccgccac
     4261 atccggagca tcgcgcggcg cctgttagtt agcgggccaa agtgcgactt attcccgcaa
     4321 gtagccggcg gccgttagac ttgccgcccc gcatcaacac ggaaatgctg tggcgctcgg
     4381 cgcaaccatc tacacattcg acatcgaact cgcggactcc gaccgcgccg tgtaccaggc
     4441 gctgaacctg cgcgtcgcgc agcatccgag cgaaacgccg gactacctga tgacgcgcgt
     4501 gctcgcgtat tgcctcgaat tttccgaagg catcgggttt tccaaaggtc tgtccgaccc
     4561 ggacgagccc gcgattttcg tgcgcgacct caccggcgca ctcatggtgt gggtggacat
     4621 cggattaccc gagcccgacc gcctgcatcg cgcgagcaag gcggcgccac gggtggccgt
     4681 gtacacacac aaggacgcgg cacagtgggc ggtgcgcatc cgcgacgcga agattcaccg
     4741 cgcggaaaaa ctcgaagcgt ttgcgttcga tcgcgcgtgg ctgacggcgg tcgtcgcgcg
     4801 gctcgagcgc cgcatgaagt tctcgctggc gcggtcggaa ggcgagatct acctcacgct
     4861 tggcgccgac accctgcaaa ccgtgctgga acgaccgctc acataacgcg gcgctcctgt
     4921 taggcgaatc acatttgggg ccctgactca gtgcaggccg cacataattt cagggtttcg
     4981 attgcggggt tccttggcgc gccctaattt cgaaccatga aaacctcaag cgttcgtcga
     5041 atggccggtc tgcttgctta tgtttcatgc ctggcagcca tgccgcttgc cgcccaggtg
     5101 ggccccatcc agcccgtact ggtgctgtcc gatggcaaac tcgaaaccaa ccacgaccga
     5161 ccgagcttcg acgaggacta ttcgatcccc gtcgacgttt acgtcgcggc cgatggcagc
     5221 gtgaccaacg tggtcgtcac gggcacatcg aagaacttca acgccgacgc cgtggcggcc
     5281 gactacatgc gcacgcgcaa gtttctgccg gcgctcgatc accagggcca ggccgtcgaa
     5341 ggcatggcca aggtcaacgt gaacatgttc aagcgcggca cgaagaaagt agttcgtgtc
     5401 acgctcaagc cgccgccgat ggcggtcgag accgaacgcc tgcgacgcat gatgtgcgcg
     5461 gatttcgtgt gggaagtgaa ccgcatgcac gaacaggccg gcgtacgcga cgcgagcctc
     5521 gaaacgatgc cttacatctc ggcgcacatg tatgccgagg gcaaggacat ggcgggcgaa
     5581 gtcgccgaga agttctggga cacctggccc aagacgctcg gcaaggtcgt ggagcgctgc
     5641 gagaaggcgc ccgagaagat gtacttcacc gaagtgctgg tgcccgcgct cgacggcacg
     5701 atgccgactc gcgacaccgt gaccgccgcc gcaccgtaaa ttggcttgag aagctgatcc
     5761 gggttgctca agatcggcgc atgttcatcc acttcgacgc cgaccgcgcc tcggactcac
     5821 cgttcgtcga acgtgtgtgg cgctgtcaca gcgaacacgg tggacccttt ctcgcagtcg
     5881 cctccagtca ctgggagctc gtggtcacgc ggctcgctgg cacgaccacc gtgacgttgc
     5941 atggacccga gacgcgcgcc cgagaagtcc actgcccggc gaatggcgag tggtttgcca
     6001 ttcgtttcaa ggccggcacg ttcatgccgc gcctgcctgt cgcgaatctg ctgaacgggc
     6061 aggacgtgaa cctggcgccc gcgtttcgcg gccggttccg gctcgaaggg ggtgcctggg
     6121 aaattcccga tttcgagaac gccgagactt tcgtggcgcg ccttgcccgg cgcggaatca
     6181 ttacgcgcga tccggccgtt gaagccgcgc tgggtggaga cgaactcgcg ctgacgcgcc
     6241 gctcggccca gaggcacttt ctgcatgcga ccggcatgag tttcacggcc atgaagaaca
     6301 tcgagcgtgc gcgcgccgcc acgcaactgc tacgcgccgg cgcgagcccc accgaggccg
     6361 cgttcgagac cggattcttc gatcagcccc atctcacgcg gtcgctgcgc caactcatcg
     6421 gactcacgcc cgcgcgcatc gcgcgcgagg aacgccagtt gtcgtttctg tacaagacct
     6481 gaggcgaacg ccgccactag ccttctctcc ccggttggag ggtgtcgatg aacaagcgag
     6541 gcaggatgtg gtggttgggc tggtcgatga gcgcgctggt ggttcttttt ctgctgttgg
     6601 actccggcat gaagctcgcg gccgtgcgcc cggtgctcga ggccggcatg cagatcggat
     6661 ttcccggcga agcgatggca cgcaagctcg ggttgctgct gctcatttgc acggcgctgt
     6721 atgtgtggcc cgccacttcg gtgctgggcg cgatcctggt caccgggttc cttggcggcg
     6781 cggtggcaac gcacgttcgg ctcggcaatc cgctcttctc gcacgtcctg ttcggcgtgt
     6841 acgtgggcgt tctgatgtgg ggcggactgt tcctgcgaga ggaacgcgtg cgtgcgctgc
     6901 tgccggtcct gaagcccgtg agcgcgggct agcgcctaca tcggggattg ctggcgcaga
     6961 tactgatcgc gcttcatggc gatgtagtcg tcgtgcttga tttcacgtag ctggcgcgcg
     7021 aaacgctccg tatcggcaaa ccagatttcg aggcttcgct cgaagcgctg gggttccggc
     7081 atgttgaggg aacgtagata ggcatcgaag gcgaggtagt accgcatggt gtttcgctcg
     7141 agggcgccgc gtgtgccgcg caccagttcc ggggcatcgc cctcgtcctg gatccacgtg
     7201 aaccctactt tgtcgcgacc cgtggtcgag aagtacacgc cgctggcgta tcgcgccagc
     7261 gctccctgcg tgtacgaata ctggatgtgc atgaacgtgc gatcgtcggg gagcgcaacc
     7321 agttcgagcg cgatgcggta atcgctggtg ccgagcggcc catccgccgc gacgagctcc
     7381 acgccgaaga aatccgcggt cgacgccttc acctcgtacg agaaggaaat ctgatgtgtg
     7441 tcgtcgagcg gctggttgat gttcttgccc accgccgcgg acaatcgcgg ctctccttca
     7501 tccacattgg cgcggcaata ctgcacgttg agatgcagga tcatcgactc gcaccagttt
     7561 tccgcttttg cgaacgcggc tgccacggtg gcgtacggat attggatcac cgcataggcg
     7621 tcaccgcggg gcgcactgtt gctctcgttc gattcgacga gcaggccacg ctggatcgag
     7681 ctgttttcga gttggccacg caaggcctcg taacgcgcgc gtaatgccga ggccgggtcg
     7741 gagggcgtcg cggccagcga ggccggggcg cacgttaccc acgcgacaag gcatgccgcg
     7801 attcgaagga gactggggaa agcagggttt ggcatcggtt ctcggactcg tgccagcgag
     7861 tgtccgcgca tgtgtgcgcg agggctatag gcttaagcct atagctgcat ggcggtctgg
     7921 ccggccttcg ccgccgctcg ccatgcagct tggggattcg cctacttcgg acgccccgaa
     7981 aagcccgctg cggtacgcaa tgcgtcggcg tccagtagtt tgccttcgag catgatgacg
     8041 cgtgtattgc gcagatcgcc gatgcgctgc gaagggtcac cttcgacgag caccaggtcc
     8101 gcgactttgc ccggcttgat ggaacccgtg cgtttgtcct ggcccacgag cttcgccgga
     8161 acgatggtcg ccgcggccag tgcctcagcc ggcgtgaacc ccgcttcgac atagatctcg
//
LOCUS       DATDEU010000602         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_994950, whole genome shotgun sequence.
ACCESSION   DATDEU010000602 DATDEU010000000
VERSION     DATDEU010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994950"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..637)
                     /locus_tag="VIV63_14420"
     CDS             complement(110..637)
                     /locus_tag="VIV63_14420"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6125842.1"
                     /translation="MNPPDLQQIRAAVAQDPRNAELRYLLGAELAQQKEYDAAVVEMS
                     TALQLDPLLHFARFQLGLLYLTMAQPNQSLETWAPLESLGETAALKFFKRGLEALIRD
                     DFDTCVGMLRHGIELNTQNAPLNHDMTLVIDRVGEALVPASRPAAAAPAPAMGAANDV
                     RTDFSLYGDPGGTKH"
     gene            complement(713..1657)
                     /locus_tag="VIV63_14425"
     CDS             complement(713..1657)
                     /locus_tag="VIV63_14425"
                     /inference="COORDINATES: protein motif:HMM:NF019610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin"
                     /protein_id="HEY6125843.1"
                     /translation="MNPARRSLLQMLGCAPLVSCSKSVPGVAAATGTDAISKIKPLFK
                     TKRPPEPGDWLADHKEPGQTYQQFRAATPAPAVSHYSTLRIVPIGTLSNGQQQVMRVV
                     TDFLEPFFGMRLALNAAVPLEAIPVDAQRQLTLEGPPQLLTTYLLNSVLMPLRRPADA
                     AVLAITAADLWPGPGWNFVFGQASLKERVGVWSMARNGDADDREAMRKLCAIRTTMTA
                     THETGHMFGIRHCTAYECGMNGSNHSEERDRQPLEFCPECQPKLWWTCGLDPLARSRA
                     LAAVARRHEIPSLISAFDQQVRALENVSQDSPGGLGRA"
     gene            complement(1661..>2359)
                     /locus_tag="VIV63_14430"
     CDS             complement(1661..>2359)
                     /locus_tag="VIV63_14430"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY6125844.1"
                     /translation="VEADGKQFRNLIARFGPREGPLIVIGAHYDSCGDTPGADDNASG
                     VAGLLELARLLALQPPKHSVELVAYTLEEPPYFRTESMGSFVHARSLAQAKREVRLML
                     SLEMIGFFRDGPDTQDYPLAPLGLLYPDRGDFIAIVASFGDFRATRRVKALFRGASDL
                     PAVSINSPRSVQGVDFSDHASYWHFEMPAMMITDTAFMRNPNYHQAGDTPDTLDYVRM
                     AKVVRAVHAVAMEF"
BASE COUNT          417 a          727 c          803 g          412 t
ORIGIN      
        1 tggcgctgcg tttcggaagt gttgccgcgg gagttcgcac gagaccgcgt gagagtgcga
       61 accagctcgg ccagcgagcg tagcgggtcg agcatgaggg cgggtcaggt cagtgtttcg
      121 tgccgcccgg atcgccatac agcgagaaat ccgtgcgcac gtcgttcgcc gcccccatcg
      181 cgggagccgg cgccgcggca gccggccgcg aggcgggcac cagcgcttcg cccacgcgat
      241 cgatgaccag cgtcatgtcg tggttgagcg gcgcattttg cgtattcaat tcgatgccgt
      301 ggcggagcat gccaacgcag gtatcgaaat cgtcccggat cagcgcttcg agtccacgct
      361 tgaaaaattt gagcgcggcg gtttcaccga gcgattcgag cggcgcccac gtttcgagcg
      421 actggttcgg ctgagccatg gtcaggtaga gcaggccgag ctggaaacgc gcgaaatgca
      481 gcagcggatc gagctgcagc gccgtgctca tttccacgac ggcggcgtcg tattcctttt
      541 gctgagcgag ctcggcgccc agcaggtacc ggagttcggc attgcggggg tcctgcgcca
      601 cggcggcgcg aatctgctgc agatctggcg ggttcatggg ccggtagcat acctccgggg
      661 aatccccggc gcgaatccac attcggtctt atggaaagcc gcggtttgct aactacgcac
      721 ggcctaaacc gcccggagaa tcctgggata cattttcgag cgcccgaacc tgttgatcga
      781 aggcgcttat caacgagggg atttcatgcc ggcgggccac ggcggccagc gcccggctgc
      841 gagccagggg atcgaggccg caggtccacc agagcttggg ctggcattcc gggcagaatt
      901 cgagtggttg gcggtcgcgc tcctcggagt gattcgagcc attcatgccg cattcgtacg
      961 ccgtgcaatg ccggatgccg aacatgtgac cggtttcgtg cgtcgcggtc atcgtcgtac
     1021 ggatggcgca caacttgcgc atggcttccc ggtcatcggc atcgccattt cgcgccatcg
     1081 accacacgcc gacgcgttct ttcagcgacg cctgtccgaa cacgaaattc cagcccggcc
     1141 cgggccacag gtccgcggcg gtgatcgcga gcaccgcggc gtcggccggc cgccgcagcg
     1201 gcatcagcac cgagttcagt agataggtcg tcagcagctg cggtgggcct tccagcgtga
     1261 gctggcgctg cgcatcgacc ggaatggctt cgagcggcac cgctgcgttg agagccagcc
     1321 tcatcccgaa gaagggctcg agaaagtcgg tcaccacgcg catgacctgc tgctggccgt
     1381 tgctcaaggt tccgatgggg acgatgcgca gcgtcgaata gtggctgacg gcaggcgcag
     1441 gcgtggcggc gcgaaattgc tggtaggtct gaccgggttc cttgtgatcc gccagccagt
     1501 cgccgggttc gggagggcgc ttggttttga acagcggttt gatcttcgaa atcgcatcgg
     1561 tgccggtggc cgctgcgaca ccgggcacgg atttcgagca cgacacgagc ggcgcgcagc
     1621 cgagcatttg cagcaacgat ctgcgcgcgg gattcatgct tcagaactcc atcgccacgg
     1681 cgtgcacggc gcgcacgacc ttggccatgc gtacgtaatc gagagtgtcc ggcgtgtcgc
     1741 ccgcctggtg atagttcggg tttcgcatga acgccgtgtc ggtgatcatc atcgcgggca
     1801 tctcgaagtg ccagtaactc gcgtgatccg aaaaatcgac gccctggacc gagcgtggcg
     1861 agttgatgga gaccgcaggc agatccgaag cgccgcgaaa cagcgctttg acccggcgcg
     1921 tcgcgcggaa atcgccgaaa gacgccacga tggcgatgaa gtcgccccgg tccgggtaca
     1981 acaggccgag cggggcgagc ggatagtcct gagtgtccgg accatcgcga aagaagccga
     2041 tcatctcgag cgacagcatg agcctgactt cgcgcttggc ctgcgccagc gagcgcgcgt
     2101 gcacgaagct gcccatggac tcggtgcgga aatagggcgg ctcttccagg gtgtaggcca
     2161 ccagttccac cgaatgcttc gggggctgca gcgccagcag gcgcgccagt tcgagcaggc
     2221 ccgcgacgcc gctggcgttg tcatcggcgc cgggcgtgtc gccgcaggaa tcgtagtgcg
     2281 caccgatcac gatcagcggc ccctcacgcg ggccgaaccg cgcaatgaga ttgcggaatt
     2341 gtttgccgtc cgcctccac
//
LOCUS       DATDEU010000603         2717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_264567, whole genome shotgun sequence.
ACCESSION   DATDEU010000603 DATDEU010000000
VERSION     DATDEU010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264567"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VIV63_14435"
     CDS             <1..437
                     /locus_tag="VIV63_14435"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6125845.1"
                     /translation="PPPAPQKEVATGTCVLGPTLHFKGELYGEEDLEFQGQLEGSIEH
                     TRSLSIGKEGGVTGNIKAKFVIVEGRVDGDIFATESVSIRQSAKVKGNIYAPRVGIAD
                     GANFSGSVDMNHKGAAVATRPATVQVALSDPAAERLLTKAMG"
     gene            complement(391..1554)
                     /locus_tag="VIV63_14440"
     CDS             complement(391..1554)
                     /locus_tag="VIV63_14440"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6125846.1"
                     /translation="MKLLRPLFVWIGLSVSCAAGAADAAPFDVAIRDLGRTWLIDNGG
                     VGLSIGVYENNQRHFYNFGTAQLDGNKSPTKDTIYEIGSIAKTMAGQLLARAVIEGRA
                     ALDDEVTKYLDEPYPNLESGGEKLRLVHLANMTSQLMDNIPDLSQVRNVPNEPLAVTR
                     MRVLERYKRADFLRELHVVAPRRMPGIDLSQSNVASMLLGVVLEKIYGEPFDVILTRE
                     SEKPLRMANGTAPSVKLLARGYTDNNDALPPFSAPMQYATGSLRYSTDDLLKYAAWQM
                     VERDASVKFAHKPTWTTQDKRESVGIFWVIGESAAGRRLQYSGGTYGFASFCDLYPDA
                     KVAIVLLSNKAADGAQGTLRALSAKIVQLAAPAAPRGDVSPSPSSAVAPPPGR"
     gene            complement(1618..>2717)
                     /locus_tag="VIV63_14445"
     CDS             complement(1618..>2717)
                     /locus_tag="VIV63_14445"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEY6125847.1"
                     /translation="LFSCDNCQQILFFENSRCMNCGMTVGYCVELAKIVTLQHQTGSA
                     QGSVYEVFFPNQGLRHYRQCKNGRDLDACNWLVPAESQQPFCNSCRLTEIAPDLSDPV
                     NRGAWAGIEAAKRRMLYTLYSLKLPVVSRQEWPNEGVAFHFLRSTQGEPVMTGHDEGV
                     ITLNIAEADSAFRENMREKMGEAYRTVLGHLRHEIGHYYWDRLIRNSNSLQPFRNLFG
                     DDSLDYDEAVKRHYEQGAPANWSESYISAYATMQPWEDWAETWAHYLHMLDTLETAKS
                     HALTIRIPSAERTGDHISTDALAFRDFESLAAGWNAVTLALNSLNRSMGLKDAYPFVL
                     SPKVLEKLRFVHELIQSRRAEQKPENLRAAG"
BASE COUNT          477 a          871 c          863 g          506 t
ORIGIN      
        1 cgcccccgcc cgccccgcag aaagaagtcg ccaccggcac ctgcgtgctc ggcccgacgc
       61 tgcatttcaa gggcgagctc tacggtgagg aagacctcga attccagggc cagctcgaag
      121 gttcgatcga acacacgcgc agtctttcca tcgggaagga aggcggcgtg accggcaaca
      181 tcaaggccaa gttcgtgatc gtcgaaggcc gcgtcgatgg cgacatcttc gccaccgagt
      241 cggtttcgat ccgtcagtct gccaaggtga agggcaacat ctacgcgccg cgtgtcggca
      301 tcgcggatgg cgccaatttc tccggtagcg tcgacatgaa tcacaagggc gcggccgttg
      361 cgacccgtcc ggccaccgtg caggttgcgc tcagcgaccc ggcggcggag cgactgctga
      421 cgaaggcgat gggctgacat cgccgcgcgg cgctgccggc gccgccagct gaacgatctt
      481 cgcggacaac gcgcgcaggg ttccctgcgc gccgtccgcg gccttgttcg acagcaggac
      541 gatggcgact ttcgcgtccg ggtagaggtc gcagaagctc gcgaaaccat aagtaccgcc
      601 cgagtactgc aatcgccgcc ccgcggcaga ttcgccgatg acccagaaga tacccaccga
      661 ctcacgcttg tcctgcgtgg tccaggtggg cttgtgcgcg aacttcaccg acgcatcgcg
      721 ctccaccatc tgccacgcgg cgtatttcag cagatcgtcg gtgctgtaac gcagcgaacc
      781 ggtcgcgtac tgcatcggtg ccgagaatgg cggcaatgcg tcgttgttgt ccgtgtaccc
      841 acgcgcgagt agtttgacgg acggcgccgt gccattggcc atgcgcaggg gcttctcgct
      901 ttcgcgcgtc aggatgacgt cgaacggctc tccatagatc ttctcgagca ccacgccgag
      961 cagcatgctc gcgacgttcg actgtgaaag gtcgatgccc ggcatgcggc gcggcgccac
     1021 cacgtggagt tctctcagga aatcggcgcg tttataacgc tccagcacac gcatgcgcgt
     1081 gacggccagc ggctcgttgg gcacgttgcg tacctggctc aggtccggaa tgttgtccat
     1141 gagctgcgag gtcatgttgg ccagatgcac caggcgcagt ttctcgccac cactctcgag
     1201 attcggatag ggctcgtcca ggtacttcgt gacttcgtcg tcgagcgcgg cccggccctc
     1261 gatgaccgcg cgcgccagca actgcccggc catggtcttg gcgatcgagc cgatctcgta
     1321 gatcgtgtcc ttggtcggcg acttgttgcc gtcgagctgc gcggtaccga agttgtagaa
     1381 gtgccgctgg ttgttttcgt agacgccgat gctcaggccc acgccaccgt tgtcgatgag
     1441 ccacgtgcgg cccaggtcgc ggatggcgac atcgaacggc gcggcgtccg cggccccggc
     1501 agcgcacgac accgaaagtc cgatccaaac aaacaagggg cgcaggagct tcatgggcgc
     1561 gcatcctcgc gcgtaagcac gcgcaagaca acagcgcccg cgtccgcgaa cggcccttca
     1621 acctgccgcg cgaagattct ccggcttttg ctctgcgcgc cgcgactgga tgagctcatg
     1681 gacgaagcgc agcttctcca gcaccttcgg cgacaacacg aacggatacg cgtccttgag
     1741 tcccatgctg cgattcagac tgttcaacgc gagcgtcacc gcgttccatc ccgcggccag
     1801 cgattcgaaa tcgcggaatg ccagcgcgtc ggtggaaatg tgatcgccgg tccgctcggc
     1861 gctcgggatg cgtatggtga gcgcgtgact cttggcggtc tcgagcgtgt cgagcatgtg
     1921 caggtagtgc gcccaggttt ccgcccagtc ttcccacggt tgcatggtgg cgtaggcgct
     1981 gatgtatgac tccgaccagt tggccggcgc accctgctcg taatgccgct tcaccgcttc
     2041 gtcgtagtcg agcgagtcgt cgccgaagag gttgcggaac ggctgcagcg aattgctgtt
     2101 ccggatcagc cggtcccagt aatagtggcc gatctcgtgg cgcagatggc ccagcacggt
     2161 gcgataggcc tcgcccatct tctcgcgcat gttttcgcga aacgcggaat ccgcttccgc
     2221 gatgttgagc gtgatgacgc cctcgtcgtg cccggtcatg acgggctcgc cctgggtgct
     2281 gcgcaggaaa tgaaacgcca cgccttcgtt cggccattcc tggcgcgaga ccacgggcag
     2341 cttcagcgag tacagcgtgt agagcatgcg gcgcttggcc gcctcgatac ccgcccacgc
     2401 accgcgattc accggatcgc ttaagtccgg cgcgatctcc gtgaggcggc aggaattgca
     2461 gaacggctgc tggctctcgg ccggaaccag ccagttgcac gcgtcgaggt cgcggccgtt
     2521 cttgcattga cggtagtggc gcagaccctg gttcgggaag aacacttcat ataccgagcc
     2581 ctgtgcggag cccgtctggt gctgcagcgt cacgatcttc gcgagctcga cacaatagcc
     2641 cacggtcatg ccgcagttca tgcaccggct gttctcgaag aacaggattt gctggcagtt
     2701 gtcgcaggag aagagtt
//
LOCUS       DATDEU010000604         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_410027, whole genome shotgun sequence.
ACCESSION   DATDEU010000604 DATDEU010000000
VERSION     DATDEU010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410027"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /gene="zwf"
                     /locus_tag="VIV63_14450"
     CDS             <1..1252
                     /gene="zwf"
                     /locus_tag="VIV63_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY6125848.1"
                     /translation="LKEDALADLLTRISYAPFEADDGSAKGTEALQKALAGNNRILYF
                     MATSPKFFGPTCLSLGAAGLACEFCRVVLEKPIGHDYTSSVGINEAVGQVFHEDRIFR
                     IDHYLGKEAVQNLLALRFANSLFEPLWNASAIDNVQITVAETVGLEGRWGYYDDSGAL
                     CDMVQNHMLQLVALVAMEPPHQFEPNEVRNEKTKVLRSLRPITAATAAATTVRGQYTA
                     GSAGGAAVPGYNQETGAKGNSDTETFVALRAEIDNWRWAGVPFYLRTGKRMQSRRTDI
                     AIQFKSVPHNIFAADEPTLQANRLVIHLQPQESVTLEVLSKQPGLEGMRLREMPLELS
                     LPKDESGGRTRIAYERLLLDVLKGNTTLFVRRDEVEAAWHWIDGIQTSWRSTRQLVKP
                     YPAGTWGPTAAIALTERHGHSWYE"
     gene            1255..>2231
                     /locus_tag="VIV63_14455"
     CDS             1255..>2231
                     /locus_tag="VIV63_14455"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008038612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY6125849.1"
                     /translation="MATTQKLHDTIIEVTERIGGRSAAARGEYLERTRKRKLVEPARG
                     KLACANLAHVMAASGEDKAKLQQSVIPNIGIVTSYNDMLSAHRPYEPYPEVIRAAARV
                     AGATAQVAGGVPAMCDGVTQGRAGMELSLFSRDVIALATALALSHEAFDGAMLLGICD
                     KIVPGLFIGAASFGQMPIILTPGGPMPTGMANKAKAEVRQRYAEGLATRDELLAAEMG
                     SYHDAGTCTFYGTANSNQMLMEMMGLHVPGAAFVAPNTPLRHALTVAATQRVAQTTAL
                     GSSYLPFVEVIDERSFVNAIVGLMATGGSTNHVIHLVAMARAVGLTVTW"
BASE COUNT          415 a          758 c          709 g          349 t
ORIGIN      
        1 cctcaaggaa gacgcgctgg ccgacctgct gacgcgaatc agctatgcgc cgttcgaggc
       61 cgacgatggc agcgcgaagg gcaccgaggc gctgcagaag gcgctggcgg gcaacaaccg
      121 catcctctat ttcatggcga cgtcgcccaa gttcttcggc ccgacctgcc tgagccttgg
      181 tgccgcgggt cttgcctgcg agttttgccg ggtcgtgctc gaaaaaccca tcggccacga
      241 ctacacctcg agcgtcggga tcaacgaggc cgtgggccag gtcttccacg aagaccgaat
      301 cttccgcatc gaccactacc tcggcaaaga agcggtgcag aacctgctcg cgctgcgatt
      361 cgcgaactcg ctgttcgaac cgctctggaa tgcgtcggcg atcgacaacg tgcagatcac
      421 cgtcgcggaa accgtcgggc tcgaaggtcg ctggggctac tacgacgatt ccggcgccct
      481 gtgcgacatg gtgcagaacc acatgttgca gctcgtggcg ctggtcgcga tggagccgcc
      541 gcaccaattc gagcccaacg aagtgcgcaa cgagaagacc aaggtgctgc gcagtcttcg
      601 tccgatcacc gcggctacgg ctgccgcgac cacagtgcgc ggtcaataca cggcaggcag
      661 cgctggcggc gccgccgtac cggggtacaa ccaggagacc ggggccaagg gcaacagcga
      721 tacggaaacg ttcgtcgcgc tgcgcgcgga gatcgacaac tggcgttggg ccggcgtgcc
      781 gttctacctg cgcaccggca agcgcatgca gtcgcgccgt accgatatcg ccatccagtt
      841 caagtccgtg ccccacaaca tttttgccgc cgacgaaccg acgctgcagg ccaaccggct
      901 cgtcatccac ctgcaaccgc aggagagcgt gacgctcgag gtcctcagca aacaaccggg
      961 cctggaaggg atgcgccttc gcgaaatgcc gctcgagctg tcgctgccca aggacgagag
     1021 cggcggccgt acgcgcatcg cctatgagcg cctcctgctc gacgtactca agggaaacac
     1081 cacgctgttc gtccggcgcg acgaggtcga agcggcgtgg cactggatcg acggcattca
     1141 gacgagctgg cgctcgacgc gccaactggt caagccgtat cctgcaggca cctgggggcc
     1201 cacggccgcg atcgctctca ccgagcgaca cggccatagc tggtacgaat aagcatggct
     1261 accacccaga agctccacga caccatcatc gaagtcaccg aacgtatcgg cggccgctcc
     1321 gccgccgcgc ggggtgaata cctggagcgt acgcgcaagc ggaagctcgt cgagccggcg
     1381 cgcggcaagc tggcctgcgc aaaccttgcg cacgtcatgg ccgcctcggg agaagacaag
     1441 gcgaagctgc agcagtcggt cattccgaac atcggcatcg tcacttccta taacgacatg
     1501 ctctcggcgc atcggcctta cgagccgtat ccggaagtca ttcgcgccgc ggcgcgtgtg
     1561 gccggcgcca cggcgcaggt cgcgggaggc gtgccggcca tgtgcgacgg cgtcacgcag
     1621 ggccgcgccg gcatggaact ctcgctgttc agccgcgatg tgatcgcgct cgccacggca
     1681 ttggctttga gccacgaggc cttcgacggc gccatgttgt taggcatctg cgacaagatc
     1741 gtgccgggcc tgttcatcgg cgctgcctcg ttcggccaga tgcccatcat cctcacgccc
     1801 ggcgggccca tgcccaccgg catggccaac aaggccaagg ccgaggtgcg ccagcgatac
     1861 gccgagggcc tggcgacgcg tgacgaactg ctggccgccg agatgggttc ttaccacgac
     1921 gcgggcacct gcacgttcta cggcacagcc aactccaacc agatgctcat ggagatgatg
     1981 gggctgcacg tgcccggtgc ggcattcgtg gcgcccaaca cgccgctgcg ccatgcgctc
     2041 accgtcgcgg ccacgcagcg cgtcgcgcag accacggcgc tcggttctag ctacctgccg
     2101 ttcgtcgaag tcatcgacga gcgttccttc gtcaacgcca tcgtcggcct catggccacc
     2161 gggggctcca ccaatcatgt gattcacctc gtggccatgg cccgggcggt gggcctgacc
     2221 gtgacgtggg a
//
LOCUS       DATDEU010000605         5947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_850268, whole genome shotgun sequence.
ACCESSION   DATDEU010000605 DATDEU010000000
VERSION     DATDEU010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850268"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /gene="tadA"
                     /locus_tag="VIV63_14460"
     CDS             complement(<1..410)
                     /gene="tadA"
                     /locus_tag="VIV63_14460"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY6125850.1"
                     /translation="MRLALEAARGAELAGEVPVGAVLVQGDSVLAVSANRPIARHDPT
                     AHAEIEALRAGGQAAGSYRLNGTTLYVTLEPCVMCASAIVHARVARLVFGAWDPKAGA
                     AGSTTNVFTMPSMNHRVDVFGGVLMEESARLLNG"
     gene            518..1225
                     /locus_tag="VIV63_14465"
     CDS             518..1225
                     /locus_tag="VIV63_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6125851.1"
                     /translation="MNTAEKNPAAADILEQPLEPRRPGEANAIANEIDIALSPITLYA
                     DALLERAELDSQARQYVGNIRRAVDDLTHAVARLRELDRPRAPDPDAGSNRARTPAPA
                     ARSLRVLLIDDDPSLIESMRSALVDEGHKVTTANGGQAGIDAFRAAFASHMPYDIVIT
                     DLAMPDVDGRQVVANLRATSPGTPIVLLTGWRHQLADRVERPLHVDRLLGKPPRIREL
                     RMALAELTGRRATDHLK"
     gene            complement(1234..2094)
                     /locus_tag="VIV63_14470"
     CDS             complement(1234..2094)
                     /locus_tag="VIV63_14470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6125852.1"
                     /translation="MTLTALCLVLLAALTHAIWNLSAKYAAESRHFVWLFSAGSVLVY
                     GPVVLAVVFVEQPVFETRHWLALAATSVLHLLYSLSLQTGYRHSDLSVVYPIARGTGP
                     LLSFIGAGLLLGEALTAQSVAGLVLIVSGILLVAGIVGHHRRAPRVGVFYGVLTGALI
                     AAYTLNDGWAVKVLLISPVVIDFTGNFFRMAVLAPRAWADRARVAVEARVYRKPIIIV
                     SVLGPLGYILVLFAMRLAPVSHVAPARELSTLVGAWFGSRLLREDSGPWRIAGAALIV
                     AGVIALSVPA"
     gene            2444..>5947
                     /locus_tag="VIV63_14475"
     CDS             2444..>5947
                     /locus_tag="VIV63_14475"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6125853.1"
                     /translation="MRKSSRQRTFGGRHGDRFMGSSAGLAALLLSSTPLATAQNADQA
                     PPVPPAPTAPAPEAAPETAPATASELEEVVVRGTRASQQASIERKKRASTAVDSIAAE
                     DVGKFPDQNVNEAISRVAGVALDRGDNGEGQGISVRGHGMEATRVDIDGMTVLNTSGA
                     LSNGTGAGGGRAADLRELPSAIIKSIDVFKGTTAAMTEGSLGGSVHIETRNGLDFDKP
                     FMQFSMDGQQNSISEKLTPTGSAIFARKFMDDRLGFFANVNYSRFETTTDLQQPQTSG
                     NAGPFRNADFDQSPDKTFVYDPNIVDPTATAGNFRVLGAGGVPVYASLSPIDILTRSA
                     AAASPAACLAAFPALTTDQLNAIPGGDQVLGTVSTSAVSAANNRTAAQLEQTNGLQTC
                     LNQWNDYAPSLIRSLPRRTEEERYSGDFRFDFRVTDDLTVYAAYQQANRHGTSVDNTL
                     NLGSPGYNQVGSFTQTAPPANSNPLISPRTVLTGLGAGYYSTGLCGAPTVTGTGAAQT
                     TTGCGVASNMTNVVVDDTHHVTSFHLADGNANIDAINYDTDIETWNWQTGLHFNREGL
                     KVDLMYGDSGSTWQRAQLRTAVNYTYGGVDAHITPSGLWSYDLPSGLDLATLPFANLN
                     PVIARNASAASTVQAASPAYTAAQAAQWGNNFTLTWRPQMSDDSERQAKMDFTFDVQE
                     KLPFVASFQSGFQRRDRSGNGWGGGGYTLRPGTGNVGAAGYVAPVVVPTENLTVNYRS
                     CMPTATSTQPCQYGYVAGTTVGTNNSPVANLNNTLFGTMTFTPSDLDALISSALYTKD
                     YPFLGDYPDKGDVMTSWPYINPAIIAAGIPEQVFDFHCMKTCTANDGTVYEQQHFAYN
                     EVTDAGYFMFDFDQKLPLDMIFNGNVGTRYVITKTSATGFMTLAHTSVTAAYNPITNP
                     NAVVTTTAALNTSLEDDSKDWLPAANLNLWIKPEQLVLRYYKGKVMSRPPVANVLPSG
                     TCTVDDRNSADVNGTTDNPNTCTGRVGNPGLKPFKGENQNLTLEWYPTEDLMFSVAHY
                     RNKIITGAPINANMPASNLFGGSDAVDPVTGEPLADFSFTYPSYVNGPSGTQLGMEYT
                     AKVAFTFLPWILRHTGVDANYSELDFENFATSRDLTAGNFNPPQGQRDYFKNFTVWYD
                     DGKFNARVSYQGQSEYFDFISS"
BASE COUNT         1236 a         1992 c         1736 g          983 t
ORIGIN      
        1 aacccgttga gcagacgcgc gctctcctcc atgagaacgc ctccaaagac atcgacgcga
       61 tggttcatcg acggcatcgt gaaaacattg gtggtgcttc cggccgcacc ggctttggga
      121 tcccacgcgc cgaaaaccag ccgcgcgact cgcgcgtgaa cgatggccga ggcgcacatc
      181 acacagggct cgagcgtcac gtagagcgtg gtgccgttca gccggtagct gcccgcggcc
      241 tggccgccgg cgcgcagcgc ttcgatttcg gcatgggcgg ttggatcgtg tcgggcgatg
      301 ggccggttgg cagacaccgc gagtacggaa tcgccctgca ccagcaccgc gcccactggc
      361 acctcgcccg ccagctccgc ccctcgcgcg gcctccaggg cgagtcgcat gaaatcgacg
      421 tcattgcgta aaggcatggg ggctcgggag aagtccttag tgacttaacc ctatcaaact
      481 actagtgttt tgtgtcgccg gagcagaggt ttggaccatg aacaccgccg aaaaaaatcc
      541 cgcggcagcg gacattctcg agcagcccct ggagccccgg cgcccaggcg aggctaacgc
      601 cattgcgaac gagatcgaca tcgccctgtc gcccatcacg ttgtacgccg acgcgctgct
      661 cgaacgcgcg gaactcgaca gtcaggcgcg tcaatacgtc ggcaacatcc gccgcgcggt
      721 cgacgacctg acgcacgcgg tcgcgcgatt gcgcgaactc gaccgcccgc gcgcgccgga
      781 tccggacgcg ggcagcaacc gggcgcgtac gccagctccc gccgcgcgca gcctgcgtgt
      841 gctgctcatc gacgacgatc catcgctcat cgaatcgatg cgtagcgcgc tcgtcgatga
      901 aggccacaag gtcactaccg cgaacggcgg ccaggcgggc atcgacgcgt ttcgcgcggc
      961 gttcgcgtcg cacatgccct acgacatcgt gatcacggat ctcgcgatgc cggatgtgga
     1021 cggccgacag gtcgtggcca accttcgcgc cacatcgccc ggcacgccga tcgtcctgct
     1081 gaccggatgg cggcatcagc tcgcggatcg tgtcgagcgc ccgctgcacg tggatcgtct
     1141 gctcggcaag ccgccgcgta ttcgcgaact gcgcatggcg ctggccgaac tcacgggacg
     1201 tcgcgcgacc gaccacctga aataacgcgg gcgtcaggcc ggcacggaca gcgcaatcac
     1261 gccggcgacg atgagcgccg cgccggcgat tcgccagggc ccggaatctt cgcgcagcaa
     1321 tcgcgagccg aaccacgcgc ccaccagcgt cgacagctcg cgcgcgggcg ccacgtggct
     1381 caccggcgca agccgcatcg cgaacaacac gaggatgtaa ccgagcggtc ccagcacact
     1441 cacgatgatg atcggcttgc gatacacgcg tgcttcgacc gccacgcgtg cgcgatcggc
     1501 ccaggcgcgc ggcgcgagaa ccgccatgcg aaagaaattg ccggtgaagt cgatgacgac
     1561 cggcgagatc aacaacacct tcaccgccca gccgtcgttg agcgtgtatg ccgcgatcaa
     1621 cgctccggtc aacacgccgt agaacacgcc gacgcgcggc gcccggcggt ggtggccgac
     1681 tattccggcc accagaagga tgccactcac gatcagcacc agtccggcaa cggactgggc
     1741 cgtcaacgcc tcgcctaaca acagtcccgc gccgatgaat gacagcaacg gcccggtgcc
     1801 gcgagcgatc ggatacacca ccgacagatc ggaatggcga tagccggtct gcaggctcaa
     1861 cgaatacagc aggtgcagga ccgaggtggc ggcgagcgcc agccagtggc gggtctcgaa
     1921 caccggttgc tcgacgaaga cgaccgccag caccaccggc ccataaacga gtaccgaacc
     1981 ggccgagaac agccagacga aatggcgcga ttcagcggcg tacttggcgg agagattcca
     2041 gatggcgtgc gtaagcgcgg ccagcaacac gagacacaac gcagtcaggg tcattcgaga
     2101 tgtaactcat tatttcgaaa tcgatattcg attcggatcg cgacgctttg caaatcgatt
     2161 cgctaacacc gaaatcgagt gtgcatataa atgcgcatat cactgatgaa cgaaaccgtt
     2221 attgacaccc gtttccgggc ggtagaacat caaaaacaat caaatacgat cataagtaat
     2281 cgtattggca atcagatgat gtcaacgcgg tggtctcaac ggccgcgtcg aaacaaaaag
     2341 aatttgccgc aacagcggca cgtgaatggc ggcgccgcgt ttgaccgccc ttcgcgaatc
     2401 gacaagaacg cgcggaaatt tgcactcggg ggaggggcaa gacatgcgga aatcatcgcg
     2461 tcagcgcact ttcggtggcc gtcatggcga ccggttcatg ggatcttcgg cgggccttgc
     2521 ggctctgcta ctgagctcga cacctctcgc gacggcacag aatgccgacc aggcgccgcc
     2581 cgtgcctccc gcgccaaccg cacctgctcc cgaagccgcg cctgaaaccg cacccgcaac
     2641 cgcgtccgaa ctcgaagaag ttgtcgtccg tggaacgcgc gcgtcccaac aagcctcgat
     2701 cgagcgcaag aagcgcgcgt cgacggcggt ggattccatc gccgccgaag atgtcggcaa
     2761 atttcctgac cagaatgtca acgaagcgat ttcccgcgtg gcaggtgtcg cgctcgatcg
     2821 cggcgacaat ggcgagggcc agggcatttc ggtgcgcggc cacgggatgg aagccacccg
     2881 cgtcgacatc gacggcatga ccgtcctgaa caccagcggc gcgctgtcga acggcaccgg
     2941 cgccggcggc ggccgcgcgg cagatctgcg cgaattgccg tccgccatca tcaagagcat
     3001 cgacgtcttc aaaggcacga ccgcagccat gacggaaggc tcgctgggcg ggtcggtgca
     3061 tatcgaaacg cgaaatggtc tcgatttcga caagcctttc atgcagttct ccatggacgg
     3121 ccagcagaac tccatctccg aaaaattgac gccgaccggt tcggccattt tcgccagaaa
     3181 attcatggat gaccgacttg gcttcttcgc caacgtcaat tattcgagat tcgaaaccac
     3241 gacggatctt cagcagccgc aaacctcggg caatgccggc ccgttccgca atgccgattt
     3301 cgaccaatcg ccggacaaga cctttgtcta cgatccgaac atcgttgatc cgaccgcgac
     3361 ggctgggaac ttccgtgtgc ttggcgccgg cggtgtgccg gtgtatgcgt cgctctcgcc
     3421 catcgacatt ctcacgaggt cggccgcagc cgcgtctccg gcggcttgcc ttgccgcgtt
     3481 tccggccctg acgacggacc agttgaacgc cattccgggg ggcgaccagg tcctgggcac
     3541 tgtctcgaca tccgctgtaa gcgccgcgaa caaccgcact gcggcgcagc tggagcagac
     3601 gaacggattg cagacttgcc tgaaccagtg gaacgactac gcaccgtcgc tcattcgttc
     3661 gctccctcgc cgaaccgagg aagaaaggta ctcgggcgat ttccgtttcg acttccgcgt
     3721 gacggacgac ctcacggtct acgccgccta tcagcaggcg aaccgccacg gcaccagcgt
     3781 cgacaacacg ctcaatctgg gcagccccgg ctacaaccag gttggcagct tcacgcagac
     3841 cgcgccgccg gccaactcga acccgctgat ctcgccacgc acggtcctga ccggactggg
     3901 tgccggctac tattcaaccg gcctgtgcgg cgctcccacc gtcaccggca ccggcgcggc
     3961 gcaaacgacg accggttgcg gcgtggccag caacatgacc aacgtcgtgg tcgatgacac
     4021 gcatcacgtc acctcgttcc acctggccga tggcaacgcc aacatcgacg ccatcaacta
     4081 cgacacggac atcgagacct ggaactggca gaccggtctt catttcaacc gtgaaggtct
     4141 caaagtcgat ctgatgtacg gagattccgg ctcgacctgg cagcgcgccc agcttcgcac
     4201 cgccgtgaac tacacctacg gcggcgtgga cgcacacatc acgccgtccg gtctctggag
     4261 ttatgacctg ccctcggggc tcgacctggc gactttgccg ttcgcaaatc tgaaccccgt
     4321 catcgcgcga aacgcctccg ccgcctcgac cgttcaggcg gcgtcgcccg cctatacggc
     4381 ggcccaggcc gcgcaatggg gtaacaactt cacgctcacc tggcgcccgc agatgtcgga
     4441 tgactccgag cggcaggcga agatggattt cacgttcgac gttcaggaga agcttccatt
     4501 cgtggcgagc ttccagtcgg gctttcaacg ccgcgatcgc agcggcaatg gatggggggg
     4561 cggcggctac accttgcgac caggcaccgg caacgttggc gccgcgggat acgtcgcgcc
     4621 tgtggtggtt ccgaccgaaa acctcaccgt caactatcgc tcctgcatgc cgacggcgac
     4681 ctccacccag ccgtgccagt acggctatgt cgcggggacc acggtcggca ccaacaacag
     4741 ccccgtcgcg aatctgaaca acacgctgtt cggcacgatg acgttcacgc cttcggatct
     4801 cgacgccctg atctcgagcg ccctctatac gaaggactat ccgttcctgg gcgactaccc
     4861 ggacaagggt gatgtcatga ccagttggcc gtatatcaat ccggccatca tcgccgcagg
     4921 cattcccgag caggtgttcg acttccactg catgaagacg tgcacggcca atgacggcac
     4981 cgtctatgag cagcagcact tcgcctacaa cgaagtcacc gatgccgggt acttcatgtt
     5041 cgacttcgat cagaagttgc cgctcgacat gattttcaac ggcaacgtcg gcacgcgcta
     5101 tgtcatcacc aagacgagcg cgacgggctt catgacgctg gctcatacct cggtaaccgc
     5161 ggcttacaac ccgatcacca atcccaacgc cgtcgtcacc acgacggccg cgttgaatac
     5221 ctcgctcgag gacgattcca aggattggct gccggccgcc aatctcaatc tctggatcaa
     5281 gcccgaacag ttggtgcttc gctattacaa gggaaaggtc atgtcacgcc cgcccgtggc
     5341 caacgtgttg ccctcgggaa cctgcaccgt cgacgaccgc aattcggccg acgtcaatgg
     5401 aacgaccgac aatccgaaca cgtgcacggg acgcgtcggc aatccgggct tgaaaccgtt
     5461 caagggcgaa aaccagaacc tgacgctcga gtggtatccc accgaagacc tgatgttctc
     5521 ggtcgcgcac tatcgcaaca agatcatcac gggcgccccg atcaacgcca acatgcccgc
     5581 gtcgaatctg ttcggaggaa gtgacgcggt cgatccggtg accggggagc ccttggcgga
     5641 cttcagtttc acctaccctt cctacgtcaa cggcccgtcc ggaacccagt tgggcatgga
     5701 atacaccgcg aaggtcgcct tcacgttcct gccatggatc ctccgtcata ccggcgtgga
     5761 tgctaactac tccgaactgg acttcgagaa tttcgcgacc tcgcgggatc tgaccgccgg
     5821 caacttcaat cccccgcagg gccagcgcga ttacttcaag aacttcacgg tgtggtacga
     5881 cgacggcaag ttcaatgcgc gcgtctccta ccagggccag tccgagtatt tcgacttcat
     5941 ctcctcc
//
LOCUS       DATDEU010000606         3939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2016909, whole genome shotgun sequence.
ACCESSION   DATDEU010000606 DATDEU010000000
VERSION     DATDEU010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016909"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /gene="sdhB"
                     /locus_tag="VIV63_14480"
     CDS             <1..182
                     /gene="sdhB"
                     /locus_tag="VIV63_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="part of four member succinate dehydrogenase enzyme
                     complex that forms a trimeric complex (trimer of
                     tetramers); SdhA/B are the catalytic subcomplex and can
                     exhibit succinate dehydrogenase activity in the absence of
                     SdhC/D which are the membrane components and form
                     cytochrome b556; SdhC binds ubiquinone; oxidizes succinate
                     to fumarate while reducing ubiquinone to ubiquinol; the
                     catalytic subunits are similar to fumarate reductase;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY6125854.1"
                     /translation="RWIIDSRDEATGERLDELEDPFRLYRCHTIMNCTEACPKDLNPA
                     KAIAEIKKMMIERQH"
     gene            166..411
                     /locus_tag="VIV63_14485"
     CDS             166..411
                     /locus_tag="VIV63_14485"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY6125855.1"
                     /translation="MNASTEIGRLAWQCRRGMKELDFLLVRYLRERYPGAASEERAAF
                     VEFLELPDPDIARYLLAGDVPHDPARAALCRQLLQSN"
     gene            974..1582
                     /gene="rpoE"
                     /locus_tag="VIV63_14490"
     CDS             974..1582
                     /gene="rpoE"
                     /locus_tag="VIV63_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEY6125856.1"
                     /translation="MTVEEGDLVLVKRVQRGDKTAFDLLVRKYQHKVVKLVLRYVRNP
                     AEAEDIAQEAFIKAYRALPQFRGDSAFYTWMYRIAINTAKNSLASRDRSPIKYDLDLT
                     DPEESHGVQTKLQDPDTPEGMALTEEIRQIVNSAIDALPEELKTAIVLRELDGLSYEE
                     IAAAMECPVGTVRSRIFRAREAIDKRLREVFEGGLGRTEEMV"
     gene            1579..2307
                     /locus_tag="VIV63_14495"
     CDS             1579..2307
                     /locus_tag="VIV63_14495"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEY6125857.1"
                     /translation="MNEERDSQLSAMFDGELPGAECELLARRLTRDEALRAQWSRFSM
                     IGAALRNERGVALHDRVAWRVSSMVAQEATYGDGTVDSVTTTRTSVAASATSVQTSAA
                     RWARFARPVAGASIAAGVAAMSILFLRTQDGPEPMLASNPVPETIVLAPEATGTTVAL
                     NTAPAAERVPSNGEPERYVTPAPRSSSSIAPPARLANYVVAHSEYSGPLSRRMALLGL
                     VATDPDAALPAEPAADANGAADGP"
     gene            2297..3337
                     /locus_tag="VIV63_14500"
     CDS             2297..3337
                     /locus_tag="VIV63_14500"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEY6125858.1"
                     /translation="MARDLSHRHSSARRNNMARLAALTLLIGAGVAFGAEPKEWLDRM
                     NKALTTRNYVGVFTHVHGGRVETLRIIHRVRGRDVSERLLSLDGSGREVIREGNELIC
                     YFPDKRTVLVERRAPDGALLGALPAIDEGNSLVYEIRGGERERLLGRNTRVVALHPRD
                     EYRYGYRLWIDEQTSMPLKTQLCNQSGEVIEQIVFSTIDLPERIPDSMFKPQMDASNY
                     RWLRADRQVASSSVPALWDAMRLPPGFRMTTRSAQAMPGSSEPVAHLVFTDGIASVSV
                     FVEPRKPDSKPSEGPSRVGSSSAFSTVVDGHQVTAVGEVPPNTVELIAKQVKASKSAA
                     ATEGLGLAPRRK"
     gene            3348..3614
                     /locus_tag="VIV63_14505"
     CDS             3348..3614
                     /locus_tag="VIV63_14505"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY6125859.1"
                     /translation="MIPAGLVVVSREGCGLCDEMLHALAELERAQSLPPVTVVDVDSD
                     PELVRQFGLKVPVLLLDGSVICHYTLNSNEVLRLVQRPAAIIPR"
     gene            3683..>3939
                     /locus_tag="VIV63_14510"
     CDS             3683..>3939
                     /locus_tag="VIV63_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6125860.1"
                     /translation="MQRTRNFSIIAHVDHGKSTLADRFIQICGGLEAREMQDQVLDSL
                     ELERERGITIKAQAVTLYYKSKKDGETYQLNLIDTPGHVDFS"
BASE COUNT          694 a         1302 c         1195 g          748 t
ORIGIN      
        1 atcgctggat catcgattcg cgcgacgagg cgaccggcga gcggctcgat gaactcgaag
       61 accctttccg gctgtatcgc tgccatacca tcatgaattg caccgaggct tgtccgaagg
      121 acctcaatcc ggcgaaggcg atcgccgaga tcaaaaagat gatgattgaa cgccagcact
      181 gaaatcggcc ggctcgcgtg gcagtgccgc cgcggcatga aggaactcga tttcctgctg
      241 gtgcgttacc tgcgtgagcg ttaccccggg gcggcaagcg aggaacgcgc cgccttcgtc
      301 gaatttctcg aattgcccga cccggacatc gcgcgttatc tgctcgcggg agatgtccct
      361 cacgatcccg cccgcgccgc gttgtgccgg cagctcctcc agtcgaattg aacttacgcc
      421 ttcgcgtatc gcggccgcgg cgtggttcac gtggctgacg ctcgcctgca ttgtcacgtg
      481 gtacgccgca ttgccgtggg aagcgcgtgt caccctctgc gtggcggtat cggcggcggg
      541 catcagcagt gttcgcgggt tcgtattgct cgccggaccg cgcgcgattc gcgccatcga
      601 atggacggcg gcgggcgagt tctcggtttt tctcggaacc gctttcacgc gacttccggc
      661 gactctggca atcgggacgt tccggccggg gttgcgtttc tgggttctgc ggttcgccac
      721 cccagccggg catcgggctg tgctcatcga agaggcggcc cgcgaccctc ggtcgttccg
      781 gtgcctcagc cggtgcctcg gccggtactt gcgccggggt tccgggcgct cctcgcgccc
      841 cgctgttacc atccggccca aggtttgaag tgcgtcacga gccattaaaa atcattcgat
      901 tagacaacgc ccaaaaggaa actttcatgg gcgttcaggg tctattcagc tcgcaaggag
      961 ctggggtgct cctgtgacgg ttgaggaagg tgacctggtt ctcgtcaaac gcgttcagcg
     1021 cggtgacaag accgcgttcg acctgctggt gcgcaagtac cagcacaagg tcgtgaagct
     1081 ggtgttgcgt tacgttcgca atccggccga agcggaagac attgcgcagg aagccttcat
     1141 caaagcgtat cgggcgttac cccagtttcg tggcgacagc gccttttaca cctggatgta
     1201 tcgaatcgcc atcaacaccg ccaagaacag tctcgcgtcg cgcgatcgca gtccgatcaa
     1261 gtacgacctc gatctgactg accctgagga atcgcacggc gtgcaaacca agcttcagga
     1321 ccccgacaca cccgaaggca tggcgctcac tgaagaaatt cggcagatcg tgaactccgc
     1381 gatcgatgcg ttgcctgaag aactgaagac ggccatcgtt cttcgcgagc tcgatgggtt
     1441 gagttacgaa gagattgcag ccgccatgga atgtcctgtc ggcacggttc gctcccgtat
     1501 atttcgagcg cgcgaggcca tcgacaagcg cctgcgcgaa gtgtttgaag gcggcctggg
     1561 ccgcacagag gaaatggtat gaacgaagag cgcgacagtc agttgtcggc aatgttcgat
     1621 ggcgagttac ccggagcgga atgcgaatta ctcgcgcgcc ggctcactcg cgatgaggca
     1681 ttgcgggccc agtggtcacg tttctccatg atcggcgccg cgctgcgcaa cgaacgtggc
     1741 gttgccctgc acgatcgcgt cgcctggcgc gtgtcgtcca tggttgccca ggaagcaaca
     1801 tacggcgacg gaacggtcga ctctgtcacc accacgcgca cttcggtcgc ggcctccgcc
     1861 acgtcggtcc agacatccgc tgcacgctgg gcgcgctttg cgcgtccggt tgccggcgcc
     1921 agcatcgctg ccggtgtggc cgccatgtcc atcctgtttc tgcgtaccca ggacgggccg
     1981 gaacccatgc tcgccagcaa tccggtaccg gaaaccatcg tgctcgcgcc tgaagccacc
     2041 ggaaccaccg ttgcgctcaa caccgcgcct gccgcggagc gcgttccgtc gaacggcgag
     2101 cccgagcgtt acgtcacgcc ggcgccaaga tcctcttcga gcatcgcgcc cccggcgcgg
     2161 ctcgcgaatt acgtcgtggc gcacagcgaa tattccggac cgctctcgcg ccgcatggcg
     2221 ctgctcggtc tcgtagccac cgatcccgac gccgcacttc ccgcggaacc tgccgccgat
     2281 gccaacggag ccgcagatgg cccgtgattt gagccaccgt cattcgagcg cgcgccggaa
     2341 caacatggcg cggcttgcgg ccctgacgct gctgatcggc gcgggtgtcg ccttcggcgc
     2401 cgaacccaag gaatggctcg atcgcatgaa caaggcgctc accacgcgca actacgtcgg
     2461 cgtgttcacg cacgttcacg gtggtcgcgt cgagacgctg cgcatcattc accgtgtccg
     2521 cggtcgcgac gtgtccgaac gtctgctgtc gctcgatggt tcgggccgcg aagtcattcg
     2581 cgaaggcaac gagctcatct gctatttccc cgacaaacgc accgtgctgg tcgagcgacg
     2641 cgctcccgat ggcgcgttgc tcggcgcctt gcccgccatc gacgaaggca actcgctggt
     2701 ctatgaaatc cgcggtggcg agcgcgagcg tctgctcggc cgcaataccc gcgtggtcgc
     2761 gctgcacccg cgcgacgaat atcgctacgg ctaccggctg tggatcgacg agcagacttc
     2821 catgccgctc aagacccagc tctgcaatca gtccggcgaa gtcatcgaac agatcgtgtt
     2881 ctccaccatc gatctgccgg aacgcattcc cgattccatg ttcaagccgc agatggacgc
     2941 ctccaactac cgttggctcc gtgccgaccg gcaggttgcc tcgagctccg tgcccgcgtt
     3001 gtgggatgcc atgaggctgc cgcccggttt ccgcatgacc acccgttcag cccaggccat
     3061 gccggggtcg agcgaaccgg tggcccatct cgttttcaca gacggcatcg cctccgtgtc
     3121 ggtgttcgtc gagccgcgca agcccgacag caaaccgtcc gaaggtccgt cgcgcgtggg
     3181 gtcttcttcg gcgttctcga ccgtggtcga tggccaccag gtcacggccg tcggcgaagt
     3241 gccgccgaac accgtcgagc tcatcgccaa gcaggtcaag gcctccaagt ccgcggccgc
     3301 caccgaaggt ctgggtctgg cgccacggcg caaatgatcc tttccgaatg attccggccg
     3361 gcctcgtggt ggtcagccgc gagggctgtg gcctttgcga tgagatgctc cacgccctgg
     3421 cggagctgga acgcgcgcaa tccctgcctc ccgtcaccgt ggtcgatgtc gactccgacc
     3481 ccgagctcgt tcgtcaattc ggcctcaaag tgccggtgct gctgctcgac ggcagcgtta
     3541 tttgccatta cacgctgaat tccaacgaag ttttgcggct ggtacagcgt cctgccgcta
     3601 taatcccgcg ctgatttttg ccggcgctcc aaggcgcgag cgccttgctc gcccgatgca
     3661 tcggcgggcc gacctccacc tgatgcaacg tacacgcaac ttttcgatca tcgcccacgt
     3721 cgatcacggc aagtccacgc tcgcggaccg cttcatccag atttgcgggg gcctcgaagc
     3781 ccgcgaaatg caggatcagg tgctcgattc cctcgagctc gaacgtgaac gcggcatcac
     3841 catcaaggcg caggcggtca ctctctatta caagtcgaag aaggacggcg agacctatca
     3901 gctgaacctc atcgacacgc ccggacacgt cgacttctc
//
LOCUS       DATDEU010000607         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2162398, whole genome shotgun sequence.
ACCESSION   DATDEU010000607 DATDEU010000000
VERSION     DATDEU010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162398"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VIV63_14515"
     CDS             <1..1833
                     /locus_tag="VIV63_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6125861.1"
                     /translation="ETPVFSTNGAAGIQDVNIAAAEIDDTNTLVYGVFNDVDIRSEAR
                     HDELTTKFTQVTLEGKHSFSDAFNMRALVGFAEANHDNPVQTTLLFDANNIDNYIYDY
                     RDNSRLPLITYGTTDVTNPATWTLTQIRLRPQSTINSYQTASLDFEWVASDSFSLKFG
                     PQWKNYIFKTTEARRSNGTLANLETNIPVVAPISSYSTLTNFGDGLGMPAGSVTSWLS
                     PDLIAAVDALDLNNPALYRMGIEPALGNNNEVEEDDLGAYIQGDFSYELGSHTLRGNI
                     GVRYIETEQTSSGYTFVGGVPVLATAVNEYDDVLPSMNLVFDLSESLLMRFGASKVMT
                     RPNLGQITPGAAVSVAGNNRTVTVGNPILEPFRANSYDLGVEWYFAPESLVSLALFYK
                     DVDSFVQAQRDSRPFTDNPYGLPDSIAIAACGATAGCSPAATWEFTVPVNTPGGDVTG
                     FEVSYQQPFTFLPGVWSNFGAILNYTGVESEITYLTVAAPPAPPTVTEVTDDLTGLSK
                     HAYNATLYFDNKVFSARVAAAYRSDYLTVVPGRNGNDVEGTASTMNIDFSSSYNINDH
                     FTISLEALNLTDEVQDQWVDSTGNRLSYYHHQGIQFYLGARFKY"
     gene            1926..>2912
                     /locus_tag="VIV63_14520"
     CDS             1926..>2912
                     /locus_tag="VIV63_14520"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6125862.1"
                     /translation="MKVPERLFMNRRQIILAGTVAALAPTVAAAAKPPAGEPGDSVIE
                     LWPGGAPDGDHVTVKEEVVERLPDGPLRDRFVQHVTRPTLTLFKPRVPFNGITLLIVP
                     GGGYVRVVIDKEGVEAADWFTERGFAAAVLRYRLPADGWTHGEDSPVHDAMRAIRLLR
                     AESAKQVASPRIGVMGFSAGGHLCGRLITEPRLAYTRRDSADDLSARPDFAVLMYPVI
                     ATTGPYAHAGSAAQLVGAGVNASDLERYSPHLNAGADTPPTMLVHAADDASVPVENSL
                     LMYDALRKARVRSELHVFDNGGHGFGLRGVAGKNVAVWPTLVQNWATNHEKTA"
BASE COUNT          570 a          965 c          867 g          510 t
ORIGIN      
        1 gaaactccgg tgttcagcac caacggcgcc gccggtatcc aggacgtcaa cattgccgcc
       61 gcggaaatcg acgacacgaa tacgctggtc tatggcgtgt tcaacgacgt ggacatccgt
      121 tccgaagcgc gccacgacga actgaccacg aagttcacgc aggtcacgct ggaaggcaaa
      181 cactcgttca gcgacgcgtt caacatgcgc gcgctggtgg gtttcgccga agcgaaccat
      241 gacaatccgg tgcagacgac gctgctgttc gacgcgaaca acatcgacaa ctacatctac
      301 gactatcgcg acaacagccg cctgccgctc atcacctacg gcacgaccga cgtcacgaac
      361 ccggccacct ggacgctgac gcagattcgc ctgcgcccgc agagcacgat caacagctac
      421 cagaccgcgt cgctcgattt cgagtgggtc gcttcggact cgttcagcct gaagttcggt
      481 ccgcagtgga agaactacat cttcaagacg accgaagcgc gccgctcaaa cggcacgctg
      541 gcgaacctgg agacgaacat accggtcgtc gcgcccatca gcagctacag cacgctcacc
      601 aacttcggtg acggactcgg catgccggcc ggcagcgtca cgtcctggct cagcccggac
      661 ctgatcgccg cggtcgatgc gctcgatctg aacaatccgg cgctgtatcg catgggcatc
      721 gagcccgcgc tcggcaacaa caacgaagtc gaagaagacg acctgggcgc gtatatccag
      781 ggcgacttct cgtacgaact cggctcgcac actctgcgcg gcaatatcgg cgtgcgttac
      841 atcgagaccg agcagacttc gagcggttat acgttcgtcg gtggtgttcc ggttcttgcc
      901 actgccgtca acgaatacga cgacgtcctg ccttcgatga acctggtgtt cgacctcagc
      961 gaatcgctgc tgatgcgttt cggcgcctcg aaagtcatga cgcgtccgaa cctcggacag
     1021 atcacgccgg gagcggccgt cagcgtggcg ggcaacaacc gcacggtgac cgtgggcaat
     1081 ccgatcctcg agccattccg cgcgaattcg tatgacctcg gcgtcgaatg gtatttcgct
     1141 cccgagtcgc tcgtgtcgct cgccctgttc tacaaggacg tggacagctt cgtgcaggcg
     1201 cagcgagatt cccgtccgtt caccgacaat ccgtacggac ttccggacag catcgccatc
     1261 gcggcttgcg gcgcgacggc gggttgctct ccggcggcca cctgggaatt cacggtgccc
     1321 gtcaacaccc cgggcggcga cgtcacgggc ttcgaagtca gctaccagca gccgttcacc
     1381 ttcctgccgg gtgtgtggag caacttcggc gcgatcctga actacaccgg cgtcgaatcc
     1441 gagatcacgt acctgacggt agcggccccg ccggctccgc ccaccgtgac ggaagtgacg
     1501 gacgatctca ccggcctttc gaagcacgct tacaacgcga cgctgtattt cgacaacaag
     1561 gtgttcagcg cgcgtgtggc ggcggcgtat cgttcggact acctcacggt agtgccgggc
     1621 cgcaacggca acgatgtgga aggaaccgcg tcgacgatga acatcgactt ctcgtcgtcg
     1681 tacaacatca atgaccactt caccatctcg ctcgaagcgc tgaacctcac cgatgaagtt
     1741 caggaccagt gggtcgattc gacgggcaac cgcctgtctt actaccacca ccagggaatc
     1801 cagttctacc tgggcgcccg cttcaagtac tagttagacg gggcgccgcc ccggaagact
     1861 gaccgatcga atggggacag aagaaattct gtccccattt ttgtttgcgg cggcatactc
     1921 cgctcatgaa ggtgcccgaa agactgttca tgaaccgccg gcagatcatt ctcgccggca
     1981 ccgtggctgc ccttgccccc accgtcgccg cggcggccaa gccgcccgcc ggtgaaccgg
     2041 gcgattcggt catcgagctg tggccggggg gcgcgccgga cggcgaccac gtcaccgtga
     2101 aagaagaagt cgtcgaacgg ttgccggacg gcccgctgcg cgatcgcttc gtccaacacg
     2161 tgacgcgccc gaccctgacg ctcttcaaac ccagggtgcc cttcaacggc atcacgctgc
     2221 tcatcgtgcc cggcggcggc tatgtacgcg tggtcatcga caaggaaggc gtcgaagcgg
     2281 cggattggtt caccgagcgc ggtttcgccg ccgcggtgct gcgttatcgc cttcccgcgg
     2341 acggctggac ccatggcgag gattcgccgg ttcacgacgc catgcgcgcc atacgcctgc
     2401 tgcgcgcaga gtcggcaaag caagttgcat cgccgcggat cggcgtcatg ggcttctcgg
     2461 cgggaggaca cctctgtggc cgcctgatca cggagcccag gctcgcttat acgagacgcg
     2521 attccgcgga cgatctctct gcgcgtccgg actttgccgt gctcatgtat ccggtcatcg
     2581 cgaccacggg cccgtacgcg catgcggggt ccgccgcgca gctggtgggc gcaggcgtga
     2641 acgcttccga tctcgaacgt tattcgcccc acctcaacgc cggcgcggac actccgccaa
     2701 ccatgctggt gcacgccgcg gatgacgcga gcgttccggt ggaaaactcc ctattgatgt
     2761 acgacgcctt gcgaaaggcc cgcgtgcgca gcgagctgca tgtattcgac aacggcggtc
     2821 acggcttcgg actgcgcggc gttgccggaa aaaacgtggc cgtctggccg acgcttgtcc
     2881 agaactgggc aaccaaccat gaaaaaacag ca
//
LOCUS       DATDEU010000608         2446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1143453, whole genome shotgun sequence.
ACCESSION   DATDEU010000608 DATDEU010000000
VERSION     DATDEU010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143453"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VIV63_14525"
     CDS             complement(<1..863)
                     /locus_tag="VIV63_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6125863.1"
                     /translation="MFRTPERLKDGYDVVIIGGGGHGLAAAYYLARDHGIRDIAVLEK
                     GYLGGGNTARNTTIIRSNYLTPEGVKFYDASLALWAGLSEEFDLNLFYSQRGHFTLAH
                     SDADLRTARWRAEVNKHFGVRSEVVDAAFVKKRVPQIDLACGGHTPILGALYHEPGAI
                     ARHDAVAWGYARGADAQGVEIHQNTEVLGLDVRDGAVAGVRTSRGNIATKRVLCAVAG
                     STPRILKLIGLDSPLVIHPLQAMVSEPLKPWLDPIIVSSSLHAYVSQSARGELVMGAS
                     LDPQELHSTRST"
     gene            1010..1645
                     /locus_tag="VIV63_14530"
     CDS             1010..1645
                     /locus_tag="VIV63_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY6125864.1"
                     /translation="MAKARGTAAEPRQGPAPEAGSVTRQIGATVRRLRSSMGLTLGEV
                     AKRSQVSAGMLSRLENGDVSPSLETLGALTAALGVPLGILFGDIGKPRGGAQHVPRGQ
                     GLEVVRRGTKRGHTYHLLAADRGPRRAFEPFLVTLNDKSEVFPGFEHPGTEFIYLLEG
                     SLTYRHGDETHLLKAGDSLTFAGAVPHGPEKLMKTPIRMLSIIIYADGVEN"
     gene            1714..>2446
                     /locus_tag="VIV63_14535"
     CDS             1714..>2446
                     /locus_tag="VIV63_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY6125865.1"
                     /translation="MNSASTVAATAAAGGTVTGIKHPPGPRGLPVIGCLLPFLKNPME
                     ALTDIARTYGGLARVPIRGKYLYVLSDPEMLKEMLITHRQKYMKNVRYRHIQSLLGQG
                     LLLSEAAEWRRQRVITQPAFKPEAIDNSVSWMADHVDHFLHRWDGYADRGEIFDVEPE
                     FNRLAQLLSGRYLLGEPFADIADEFCEVAAAVKKHWPQAPRNIVRAFIPHSKELIARF
                     DTAIAALDACILGFIQRHRPTDFEGC"
BASE COUNT          446 a          804 c          790 g          406 t
ORIGIN      
        1 gtggatcgcg tcgagtgcag ctcctgggga tcgagcgagg cgcccatgac cagctcgccg
       61 cgagcactct gcgacacgta ggcgtgcagc gagctcgaca cgatgatggg atcgagccac
      121 ggtttcagcg gctcggacac catcgcctgc agcggatgga tgaccagtgg cgagtccagt
      181 ccgatgagct tgaggattcg cggcgtgctg ccggcgaccg cgcagagcac tcgcttcgtg
      241 gcgatgttgc cgcgcgaagt gcgcacaccg gcgacggcgc catcgcgcac gtccagcccc
      301 agcacctcgg tgttctggtg gatctccact ccctgggcgt cggcgccgcg cgcgtaaccc
      361 caggccacgg cgtcatgccg cgcgatcgcg cccggctcgt gatacagcgc gccgaggatg
      421 ggcgtgtgtc cgccgcaggc gagatcgatc tgcggcacgc gcttcttcac gaaggcggcg
      481 tccaccacct ccgaacgtac accgaaatgt ttgttgacct ccgcgcgcca gcgcgccgtg
      541 cgcaggtcgg cgtccgagtg ggccagcgtg aaatgaccac gctgcgaata gaacaggttg
      601 aggtcgaact cttcggagag ccccgcccac agtgcgaggc tcgcgtcgta gaacttcacg
      661 ccttcgggcg tgaggtaatt cgaacggatg atggtcgtat tgcgcgcggt attgccgccg
      721 ccgaggtagc ccttttccag caccgcgatg tcgcggatgc catgatcgcg cgccaggtaa
      781 tacgcagcgg cgagcccgtg tccgccgccg ccgatgatga cgacgtcgta gccgtccttg
      841 aggcgctccg gtgtccggaa catccgcggc tctttgcggg aaaaggcgaa tttcagaagg
      901 cgatggggca tcgaatcgcg agttgcatga gagtaaatta cgttgcgcac ggataacata
      961 cccgattgcg gcgtacaagc ccggggctgc aagagataag gagtgagtta tggcaaaagc
     1021 gaggggaacg gcggcggagc cgcggcaggg tcccgccccc gaggcgggaa gcgtgacgcg
     1081 tcagatcggc gcgaccgtgc ggcggctgcg cagctcgatg ggcctgacac tcggtgaggt
     1141 cgccaagcga tcccaggtca gcgccggaat gttgagccgg ctggaaaacg gcgacgtgtc
     1201 ccccagcctc gaaacactcg gcgccctcac cgccgcactg ggcgttccac taggcatttt
     1261 gttcggcgac atcggcaagc cccgcggcgg tgcgcaacac gtgccccgcg gccagggcct
     1321 cgaagtcgtg cgtcgcggca cgaagcgcgg ccacacctac cacctgctcg ccgccgatcg
     1381 cggcccgcgc cgcgcattcg agccgtttct cgtcacgctc aacgacaaga gcgaggtgtt
     1441 cccgggtttc gagcatccgg gcaccgagtt catctacctc ctggaaggct cgctcaccta
     1501 ccggcacggc gatgaaacgc atctgctcaa ggccggcgat tcgctgacgt tcgcgggcgc
     1561 cgtgccgcat ggacccgaga agctgatgaa gactccgatc cgaatgctgt cgatcatcat
     1621 ttatgccgac ggggtcgaga attgagctac gctccgcagc gtcgacaaca ataaaaaaac
     1681 tattctgacg ctcggggtcc acggggggcg taattgaatt cagcgagcac tgttgcagcg
     1741 actgcggcgg ccggcggtac ggtcacgggg atcaagcatc ctccgggccc gcgcgggttg
     1801 cccgtcatcg gctgcctgct tccgttcctc aagaatccca tggaggcgct gaccgacatc
     1861 gcgcgcacct acggcggact cgcgcgcgtg cccattcgcg gcaagtacct ctacgtgctc
     1921 tcggatcccg agatgctcaa ggagatgctg atcacgcatc gccagaagta catgaagaat
     1981 gtgcgctacc ggcacatcca gtcgctgctc ggccagggac tgctgctgtc cgaagccgcc
     2041 gaatggcggc gccagcgcgt catcacgcag ccggcgttca agcccgaagc catcgacaac
     2101 tccgtgagct ggatggccga tcacgtcgac catttcctgc accgctggga tggctacgcc
     2161 gatcgcggcg aaatcttcga cgtcgagccg gaattcaatc gcctcgcgca attgctgtcg
     2221 gggcgttacc tgctgggcga gccgttcgcc gacatcgccg acgaattctg cgaagtggcc
     2281 gccgccgtca agaaacactg gccgcaggcg ccgcgcaaca tcgtgcgcgc gttcatcccg
     2341 cattcgaaag agctgatcgc gcgcttcgac accgcgatcg ccgcgctcga cgcctgcatc
     2401 ctcgggttca tccagcgcca ccgccccacc gatttcgaag gctgcg
//
LOCUS       DATDEU010000609         9750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_560331, whole genome shotgun sequence.
ACCESSION   DATDEU010000609 DATDEU010000000
VERSION     DATDEU010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560331"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /gene="potE"
                     /locus_tag="VIV63_14540"
     CDS             <1..1256
                     /gene="potE"
                     /locus_tag="VIV63_14540"
                     /inference="COORDINATES: protein motif:HMM:NF007938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HEY6125866.1"
                     /translation="PANMAGVGAISLLSWVVTALGSMAIAYGFAQAGIFNQKTGGMAA
                     YAEDAYGKSGYFQVFMLYFLSLAIGNVAIGISAVGYLATFFPGMSATPIATCMSVILL
                     LWLTTIANFGGPSVTGKIGSVTVWGVILPVGLLSIIGWFWFSKETFDAAWNPQGLSIG
                     QGMGSSISLTLWAFLGMESAAQNSDAVENPKKNVPLACLFGTLGAAVIYILSTTVIQG
                     IVPNAELAESTGPFGLVYAKMFGPTVGTIVMGLAVLACLGSLLGWQFTIASTGKSAAE
                     SRMMPALFAKVNSMGAPIKGMIVLGIVQTGLALSTISPTLSEQFSALVNLAVVTNVIP
                     YIVSLSALFVMMKAAKVADNVYTRNVVIVVLGMGYSVFALYASGKDAVMGGMLVLGIV
                     YIVWGFIANRFTGELEGSAAPAAKV"
     gene            1268..2167
                     /locus_tag="VIV63_14545"
     CDS             1268..2167
                     /locus_tag="VIV63_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6125867.1"
                     /translation="MRMKSSALFAFASIALICAMPASAATLDHIKETGHIRLGYVPDA
                     KPFTSMGSSGPEGYGVQVCEAVVAQLKIQLGLQDLAVDWVAISIESRFAEVQQGNVDL
                     LCMPTSETLTNRKQVSFSLPVFAGGNRAVMRADAPAPLRHLLAGDRPDKPVWRGSPAS
                     RLLADTSYAVVSGTGTEAWLKERGAALGIKAKVTPVPDYRSGIQAVLDRKVDVFFGDR
                     AVVLGAIDAGKRDEIVVVDRLFTHEQAGLALARGDDDFRLAVDTALANIYPSQNFADL
                     YKRWFGPLDDKAKTFFLWSTPAP"
     gene            2234..4507
                     /locus_tag="VIV63_14550"
     CDS             2234..4507
                     /locus_tag="VIV63_14550"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6125868.1"
                     /translation="MRALIVDDELGHATAEGRAVRGLVEEMKGRGIEVVEATSAADGT
                     SVITSDSAIHVVLIDWRLSDSKNNDDSRAFIKTLRERNDKIPIFLMAERREASGISIE
                     VMEMVDEFVWTLEDTAAFVGGRIVASIRRYLTAIMPPLAAALFRFTQEYEYSWHTPGH
                     TGGTAFLKSPVGRVFFDFYGENLLRSDLSISVGALGSLLDHSGPMGEHERYAARVFGA
                     HRTYSVTNGTSMSNRVIFMAAVGRGQVAFCDRNCHKSIEHSLVMTGGIPNYLVPLRNR
                     YGIIGPIPPERLQKKALDASVKENALVTKGVSPRGVYAVVTNSTYDGLCYNAKRVQEL
                     LDPSVDRIHFDEAWYAYARFNPLYRDRHAMHGDPKDHKGPTIFATHSTHKLLAALSQA
                     SFLHIRDGRGAIPHSRFNESFMMQASTSPLYPIIVSNDVTAAMMDGPGGRTLTDESIQ
                     EAVAFRQTVGRAHRQFAKKKEWFFKTWNAETIKSKKGGKKVRFEDADPQQLATDPQCW
                     VLHPGETWHGFGKLEDNYCMLDPIKVSIVSPGVADKGGLDKRGVPATLVTGYLHVRGV
                     EVEKTTDFTILVLFSIGITKGKWGTLLNALLEFKRDYDRNAPISEALPHLVSSYPATY
                     GDMGLRDLADQMFDQLKSARQTHWLAEAFSTLPDLAMSPSEAYQYLVRDEIEHVPLDK
                     LANRVLATSVVPYPPGIPMLMPGEKTGADDGPYLGYLRALWEWDKRFPGFGHDTHGIE
                     TRDGVQYIQCLKADAGG"
     gene            4528..5856
                     /locus_tag="VIV63_14555"
     CDS             4528..5856
                     /locus_tag="VIV63_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6125869.1"
                     /translation="MTLFPATMLVAGNMIGTGVFLLPVNLAQVGGIAIFGWVIATLGV
                     AALGLAFAKLGQLDPQPGGPYAYARDFLGSFAGFQTNYVYWFGNWIGNIAIAVAAIGY
                     FTELVPAINGPVAALLATAGLIWLLTFANILGPRVVGALEAWTMLLAIIPILAIALFG
                     WFWFDADKFMSGWNVSGGSDMHAVSRAASMALWAYMGIESAIVSAGVIENPKRNVPLA
                     TLMGLGAAAVIYVLSSTVIMGLLPNDELRTSHAPFAAAARMAVGDWGAIVIAICAVLK
                     SIGSLGGWMLLVGQSSKAAADDGMFPKIFARVNRHGVPGYGLVIVAALMTAVLVATMS
                     PTLAEQFNHIVDLAVILIIVPYIYSAVAVVKVVHDHGVPHGNFGNYKLVALLAVTYCL
                     WVVLGGDPKTVVHAMVALLISVPLYPFFIRSMLEAKQRKSQDMIKEGAPS"
     gene            5853..7187
                     /locus_tag="VIV63_14560"
     CDS             5853..7187
                     /locus_tag="VIV63_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEY6125870.1"
                     /translation="MSIYPRSKVAAAVLLALAATSALAAEEEAKPTPPPGLPPGVDWT
                     FSLNAGFGVFGFGNSLYLTPRDEPSGDLGDNWSEGSIKAGLAGVHTSDSGGEWYGKIS
                     GVGERTFGAAPPLVGGDASSFGVEDLYIGWRSGGDKSLWDVSVGRQQYKLGHGLLLYD
                     GASEGGSRGGYWTNARSAFELTGIARFKPGNSTLEAFYLDRDELPESDTGSKLWGVNY
                     EYAIGEDTTLGATYMSWSAKESMAPERDGLDVYNLRAFTAPFPNMKQLSFELEYAQED
                     NGELLDSTAWNALAAWQFEDSWKLKLSYRYAFFEGDDPDTVENEAFDGLFTGFYDWST
                     WWQGEIAGGYLISNSNLISSQLRLSAKPTESISTGLIFFDFTLDHPESFDPLVTSSDI
                     GTELDWYMDWTINDNFIVSFIAAVAEPGEAIEQSNGRTDTFIYGMIFAAYSF"
     gene            complement(7191..7868)
                     /locus_tag="VIV63_14565"
     CDS             complement(7191..7868)
                     /locus_tag="VIV63_14565"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY6125871.1"
                     /translation="MNTEPRSSLMSRYLDPASSLGEIMFGLIMTLTFTLGAGIIIEDE
                     GKQGAHDLLIALIGCNIAWGVIDAAMYLMTELFTRGKRRRVAEAVRSAPDERGGVAVV
                     ANELDQYFEDVADETERQALYARVAAKVRVADIKPNRLTKADFMGAFTSFWLVVIASA
                     PAAIPFLIWDDARFALRVSNGILLAFLFFCGYNWARYTLGNPWTAGLIFLAGGFGLVA
                     MAIALGG"
     gene            complement(7926..8585)
                     /locus_tag="VIV63_14570"
     CDS             complement(7926..8585)
                     /locus_tag="VIV63_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6125872.1"
                     /translation="MIELYTAATPNGWKISIALEEMGLPYTVHPLTLSKLEQKEEWFL
                     KINPNGRIPAIIDRDNGDFAVFESGAILIYLAEKSGKLLPTELKGRSRVLQWLMFQHG
                     GVGPMMGQANVFFRYAPEKIPYAIERYQREVRRLLEVMERQLAGNEYIAGSEYSIADI
                     ALWSWIAGYGWSGVSIDGLPQLKRWMDLIGARPAAQRGRNIPPPADPSKVAESGRSIL
                     A"
     gene            8630..9550
                     /locus_tag="VIV63_14575"
     CDS             8630..9550
                     /locus_tag="VIV63_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125873.1"
                     /translation="MPLLIDQWLLDHWRLFAVAALFFAGVILRRARWAGVREGRWLLN
                     FVFYVGLPILIFGALTGVPLEHEHAVLPAIAICITLLCWGAAAFLARRFGLPKKSEGA
                     VALCAMSMNIAMIYPFASLKLSTAAFSQLVMFDMGHAVMAWTLTTIVACKYGGHADDI
                     PVLLRRALAAPPLWVLFLALALNLGGAPISRDLLHDVLVVGQWLVLLVPLAMGLLVSA
                     AGMRRREVVASVVLRSGLGGLAGFTLAWLFGLEGTAVGIAALAASAPVGFTAVVLSAR
                     EGLDLELAASAAAISVFLGSLWLPLAVTFV"
BASE COUNT         1718 a         3071 c         3109 g         1852 t
ORIGIN      
        1 tgccggcgaa tatggcggga gtcggagcga tctcgctgct ctcctgggtc gtcacggcgc
       61 tgggctccat ggccatcgcg tacggattcg cgcaagccgg aatattcaac cagaagacgg
      121 gcggcatggc cgcgtacgcc gaggacgcct acggcaagtc cggctacttc caggtcttca
      181 tgctgtattt cctgtcgctc gccatcggca acgtcgcgat cggcatttcg gcagtgggct
      241 acctggctac attcttcccg ggaatgtccg ccacgcccat cgcaacctgc atgagcgtca
      301 tcctgttgtt atggctgacc acgatcgcca acttcggcgg accgagcgtc accggcaaga
      361 tcgggtccgt gaccgtgtgg ggcgtcatcc ttccggtggg attgctctcc atcatcggct
      421 ggttctggtt cagcaaggag acgttcgacg cggcctggaa tccacaaggg ttgagcatcg
      481 gccagggcat gggctcgagc atctcgctga ccttgtgggc cttcctcggc atggagtccg
      541 cggcgcagaa ctccgacgcc gtcgaaaatc cgaagaagaa cgttccgctg gcctgcttgt
      601 tcggcacgct gggcgccgcg gtcatctaca tcctctcgac gaccgtcata cagggcatcg
      661 tgcccaacgc cgaactcgcc gaatccaccg gcccgttcgg cctggtgtac gcgaagatgt
      721 tcggcccgac agtcggaacc atcgtcatgg gtctcgcggt gctggcctgc ctcggctcct
      781 tgttaggctg gcagttcacg atcgcctcga ccggcaagtc ggcggctgag agccggatga
      841 tgccggcgtt gttcgccaag gtgaactcga tgggtgcgcc gatcaaggga atgatcgtgc
      901 tgggaatcgt gcagacggga cttgcgctgt cgacgatctc accgacgctc agcgagcagt
      961 tcagtgcgct ggtcaacctc gcggtggtca ccaacgtcat tccgtacatc gtctcgctgt
     1021 ccgcgctgtt cgtcatgatg aaagccgcga aagtggccga caacgtctat acgcgcaacg
     1081 tggtgatcgt cgtcctcggc atgggctaca gcgtgttcgc gctctacgcc tcgggcaagg
     1141 acgcggtgat gggcggcatg ctggtgctcg gcatcgtcta catcgtctgg ggattcatcg
     1201 cgaatcggtt cacgggcgag ctcgaaggat ccgccgcgcc tgccgcgaag gtatgaggag
     1261 aacaatcatg agaatgaaat catcggcact attcgctttc gcctccatcg cgctgatctg
     1321 cgccatgccg gcgtcggcgg ccacgctgga tcacatcaag gaaacgggcc acatccggct
     1381 gggatacgta ccggatgcga agccattcac ctcgatgggc tcttcgggcc ccgagggtta
     1441 cggcgtgcag gtatgcgagg ccgtcgttgc ccagctcaag atccagctgg gactgcagga
     1501 cctcgccgtg gactgggtgg cgatcagcat cgagagtcgc ttcgcggaag tgcagcaggg
     1561 gaacgtcgac ctgctgtgca tgccgacgag tgagacgctg accaatcgca agcaggtgtc
     1621 gttctcgctg ccggtattcg cgggaggcaa ccgggccgtg atgcgcgccg atgccccggc
     1681 gcctttacgt cacctgctcg ccggggatcg tcctgacaaa cctgtgtggc gcggttcgcc
     1741 ggcctcgagg ctgctggcgg atacgtcgta tgccgtcgtc tccggaacgg gcacggaagc
     1801 gtggctgaag gaacgcggtg ccgcgctcgg catcaaggcg aaagtcacgc cggttccgga
     1861 ttaccgctcg ggcattcaag cggtgctcga ccgcaaggtg gacgtgttct tcggtgaccg
     1921 tgcggtggta ctcggcgcga tcgatgccgg caagcgggat gagatcgtgg tcgtggaccg
     1981 gttgttcacg cacgaacagg cgggactcgc tctggcccgt ggcgacgacg acttccgcct
     2041 ggccgtggat acggctcttg ccaacatcta tccatcgcag aacttcgccg acctctacaa
     2101 gcgctggttc gggccgctcg acgacaaggc gaagacattc ttcctctgga gcacgcccgc
     2161 gccgtagagc gtccgactgc actatctaca aggagtacgc gagatgattc agcgaggcgc
     2221 caacagattg agcatgcggg cacttatcgt cgatgacgag cttggtcacg cgaccgccga
     2281 aggtcgcgct gtccgcgggc tcgtggagga gatgaagggg cggggcatcg aagtggtgga
     2341 ggcgacttcc gccgcggacg gtacgtcggt catcacctcg gactccgcga tccatgtcgt
     2401 gctcatcgat tggaggctga gcgacagcaa gaacaacgac gactcacgcg cgttcatcaa
     2461 gacgctgcgc gaacgcaacg acaagatccc gatcttcctc atggccgaac gacgtgaagc
     2521 gtccggcatt tcgatcgaag tgatggagat ggtcgacgag ttcgtctgga cgctcgagga
     2581 tacggccgcc ttcgtgggcg ggcgcatcgt ggcatccatt cgccgctacc tcaccgcgat
     2641 catgccgccg ctggcggcgg cgctgttccg cttcacgcag gagtatgagt actcgtggca
     2701 cacgccgggt cacaccggcg gcaccgcgtt cctcaaatct ccggtgggcc gcgtgttctt
     2761 cgacttctac ggcgagaacc tgctgaggtc ggacctgtcg atcagcgtcg gcgccctggg
     2821 ctcgctgctc gatcactccg gcccgatggg tgaacacgag cggtacgccg cgcgcgtgtt
     2881 cggtgcgcat cgcacgtaca gcgtcaccaa cggaacgtcg atgtcgaacc gcgtgatttt
     2941 catggccgcg gtgggccgag ggcaggtggc gttctgcgat cgcaactgcc acaagtcgat
     3001 cgagcacagc ctggtgatga ccgggggaat cccgaactac ctggtgccgc tgcgcaaccg
     3061 ttacggcatc atcggcccga ttccgccgga gcgtttgcag aagaaggcac tcgacgcttc
     3121 cgtgaaggaa aatgcgctgg tgacaaaggg cgtgagtcct cgcggcgtgt acgccgtggt
     3181 caccaactcc acgtacgacg gtctttgcta caacgcgaag cgcgtgcagg agttgctcga
     3241 ccccagcgtc gatcgcatcc acttcgacga ggcctggtac gcctacgcgc gtttcaatcc
     3301 gctgtatcgc gaccgccatg cgatgcacgg cgacccgaag gaccacaagg gtccgacgat
     3361 cttcgccacg cattccacgc acaaactgct ggccgcgttg tcacaggcgt cgttcctgca
     3421 cattcgcgac ggccgcggcg ccatcccgca ttcgcgtttc aacgaatcct tcatgatgca
     3481 ggcgtcgacc tctccgctgt atcccatcat cgtctcgaac gacgtcacgg ccgccatgat
     3541 ggacggcccg ggcggacgca cgctcaccga cgaatcgatc caggaagccg tcgcattccg
     3601 ccagaccgtg ggccgcgcac accggcagtt cgcgaagaag aaggaatggt tcttcaagac
     3661 ctggaacgcc gagaccatca agtcgaagaa gggcggcaag aaagtccgct tcgaagatgc
     3721 cgatccgcag caactcgcca ccgatccgca atgctgggtg ttgcacccgg gcgagacctg
     3781 gcacggcttc ggcaagctcg aagacaacta ctgcatgctc gatcccatca aggtgtcgat
     3841 cgtctcgccg ggagtcgccg acaagggcgg cctcgacaaa cgcggcgtgc ccgcgacgct
     3901 ggtcaccggg tacctgcatg tgcgaggggt cgaagtggag aagaccaccg acttcaccat
     3961 tctcgtgctg ttctcgatcg gcatcaccaa gggcaagtgg ggcacgttgc tcaacgcgct
     4021 gctcgagttc aagcgcgact acgaccgcaa tgcgcccatc agcgaggcgc tgccgcacct
     4081 cgtgtcgagt tacccggcga cctacggtga catgggattg cgcgatctcg ccgaccagat
     4141 gttcgatcag ttgaaaagcg cgcggcagac gcactggctc gccgaagcat tttcgacgct
     4201 gcccgatctc gccatgtcgc cgagcgaggc ctaccaatac ctggtgcgcg acgagatcga
     4261 gcacgtgccg ctcgacaaac tcgcgaaccg cgtgctggcc accagtgtcg tgccctatcc
     4321 acctggcatc cccatgctga tgccgggtga gaagaccggc gcggatgacg ggccgtatct
     4381 cggctacctg cgcgcactgt gggagtggga caagcgattc ccgggtttcg gccacgatac
     4441 ccacggcatc gaaacccgtg atggcgtgca gtacatccag tgcctcaagg cggacgcggg
     4501 cggctagtgt cccaacctgg aaagaagatg acgctgtttc cggccacgat gctcgtggcc
     4561 ggaaacatga tcggcacggg cgtgttcctg cttccggtga atctcgcaca ggtgggcggc
     4621 atcgcgatct tcggttgggt catagccacg ctcggcgttg cggcgctggg ccttgcattc
     4681 gcgaaactcg gccagctcga tccgcaaccg ggcggaccat acgcctacgc gcgggatttc
     4741 ctcggttcgt tcgccggctt ccagaccaac tacgtgtact ggttcggcaa ctggatcggc
     4801 aacatcgcga tcgcggtcgc ggccattggc tatttcaccg aactggtgcc cgccatcaac
     4861 ggacccgtgg cagcgctgct cgccaccgcc ggcctcatct ggctcctgac gttcgccaac
     4921 atcctcgggc cgcgcgtcgt gggcgctctc gaggcctgga cgatgttgct ggcgatcatc
     4981 ccgatcctgg ccatcgcctt gttcggctgg ttctggttcg acgccgacaa gttcatgagc
     5041 gggtggaatg tgagcggcgg ttcggacatg catgcggtgt cgcgcgcggc gtccatggcg
     5101 ctctgggcat acatgggtat cgaaagcgcc atcgtgtcgg ccggcgtcat cgagaatccg
     5161 aaacgcaacg tgccactcgc cacgctcatg ggcctgggcg cggccgccgt catctacgtg
     5221 ctgagttcca cggtcatcat gggcctcttg cccaacgacg agctgcgcac gtctcacgcg
     5281 ccgttcgcgg ccgccgcccg catggcggtg ggtgactggg gcgcaatcgt cattgcgatc
     5341 tgcgcggtgt tgaagtccat cggatcgctc ggcggctgga tgttgctggt cgggcaatcg
     5401 agcaaggcgg ctgccgacga cggcatgttt ccgaagatct tcgcgcgcgt gaaccgccat
     5461 ggcgttcccg gctacggcct ggtgatcgtc gcggcgctca tgaccgcggt gctggtcgcg
     5521 accatgtcgc cgacgctggc cgagcagttc aatcacatcg tcgacctcgc ggtcatcctc
     5581 atcatcgtgc cgtacatcta ttcggcggtg gccgtcgtga aagtggtcca cgaccacggc
     5641 gtgcctcacg gaaatttcgg caactacaaa ctggtggcgc tgctggcagt gacctactgc
     5701 ctctgggtcg tgctcggtgg cgatccgaag accgtggtgc acgccatggt ggcgctgctc
     5761 atcagcgtcc cgctgtatcc gttcttcatt cgctcgatgc tcgaagcaaa gcagcgcaaa
     5821 tctcaagaca tgatcaaaga aggagctccg tcatgagtat ctatccacga agcaaggtag
     5881 ctgccgcagt gctgctggcg ctcgccgcga cttcggcact ggctgccgaa gaagaagcca
     5941 agcccacgcc gccgccgggt ctgccgccgg gcgtggactg gaccttcagc ctgaatgccg
     6001 gtttcggcgt attcggtttc ggcaactcgc tgtatttgac tccgcgagat gaaccctcgg
     6061 gcgatctcgg tgacaactgg tcggaaggtt cgatcaaggc aggtctcgcc ggtgtacaca
     6121 ccagcgacag cgggggcgag tggtacggca agatcagcgg cgtcggcgaa cgcacgttcg
     6181 gcgcagcgcc gccgctcgtg ggcggcgatg cttcttcgtt cggtgtcgaa gacctgtaca
     6241 tcggctggcg ctcgggcggc gacaagagcc tgtgggacgt cagcgtcggc cggcagcagt
     6301 acaaactcgg ccacggcctg ttgctctacg acggcgcgtc ggaaggcgga tcgcgcggcg
     6361 gctactggac caacgcgcgc tcggcattcg aactgacagg catcgcgcgt ttcaaaccgg
     6421 gcaacagcac cctcgaggcg ttctacctgg atcgcgacga actacccgag tccgacaccg
     6481 gcagcaaact ctggggcgtg aactacgagt acgccatcgg cgaggacacg acgctcggcg
     6541 cgacgtatat gagctggtcg gcgaaggaat ccatggcgcc tgagcgcgac ggtctcgacg
     6601 tttacaacct gcgcgcgttc acggcgccgt tcccgaacat gaaacagctc tcgttcgagc
     6661 tggaatatgc gcaggaagac aatggcgaac tactcgactc caccgcctgg aatgcgctgg
     6721 cggcctggca gttcgaagac agctggaagc tgaagctgtc gtatcgctac gcgttcttcg
     6781 aaggcgacga tccggatacc gtggagaacg aggcgttcga tggactgttc accgggttct
     6841 acgactggag cacctggtgg cagggtgaaa tcgccggtgg ctacctgatc tccaactcga
     6901 acctcatttc gagccagttg cgcctgagcg ccaaacccac ggagtcgatc tcgaccgggc
     6961 tcatcttctt cgacttcaca ctggatcacc cggagtcgtt cgacccgctc gtcacgtctt
     7021 cggatatcgg caccgagctc gactggtaca tggactggac catcaacgac aacttcatcg
     7081 tgagcttcat tgccgccgtc gccgaaccgg gcgaggcgat cgagcagtcg aacggccgga
     7141 ccgacacctt catctacggg atgatcttcg ccgcctacag cttctgaggg tcagccaccg
     7201 agcgcgatgg ccattgcgac gagtccgaat ccgccggcca ggaatatgag gccggcggtc
     7261 cacggattgc cgagcgtgta acgcgcccag ttgtagccgc agaagaacag gaacgcgagc
     7321 aggatgccgt tcgagacgcg aagcgcgaaa cgcgcgtcgt cccagatcag gaagggaatt
     7381 gcagccggcg cgctggcgat cacgaccagc cagaagctcg tgaacgcgcc catgaagtcc
     7441 gccttggtga gacggttggg tttgatgtcc gcgacgcgca ccttggcggc gacgcgtgcg
     7501 tacaacgcct ggcgctcggt ttcgtcggca acgtcttcga agtactgatc cagctcattc
     7561 gccaccacgg ctacgccgcc gcgttcgtcc ggtgcgctgc gcacggcttc ggccactcgc
     7621 cgccgcttgc cgcgagtgaa cagctcggtc atgagataca tggcggcgtc gatcacgccc
     7681 cacgcaatgt tgcagccgat gagtgcgatg aggagatcgt gcgcgccctg tttcccttca
     7741 tcctcgatga tgatgccggc gcccagcgtg aacgtgagcg tcatgatgag gccgaacatg
     7801 atctcgccca ggctcgaggc cgggtcgaga taccgggaca tgagtgagga acgcggttct
     7861 gtgttcatcg cccgatgctc gatgccggga accgcgcccg ctatcaatct tccgcagctt
     7921 tcccgctacg cgaggatgct gcgtcccgat tcggcgacct tcgacgggtc cgcgggcggc
     7981 gggatattcc ggccgcgttg cgccgcgggg cgggcgccga tgaggtccat ccagcgcttc
     8041 aattgcggca ggccgtcgat gctgacacca gaccagccgt agcccgcgat ccagctccac
     8101 agtgcgatgt cggcgatcga gtattcggac cccgcgatgt attcgttacc ggcgagctgc
     8161 cgttccatga cctctaacaa cctgcgcact tcgcgctggt atcgctcgat ggcgtaggga
     8221 atcttctccg gcgcataccg gaagaacaca ttggcctggc ccatcatggg acccactccg
     8281 ccatgctgga acatcagcca ttgcaacacg cgcgaacggc ccttgagttc cgtgggcagg
     8341 agcttgccgc tcttttcggc cagatagatg aggatggcgc cggactcgaa caccgcgaag
     8401 tcgccattgt cacggtcgat gatggcggga atgcgtccgt tcgggttgat cttgaggaac
     8461 cactcttctt tctgctcgag cttcgacagc gtgagcggat gaaccgtgta cggcaggccc
     8521 atctcctcga gggcgatcga tatcttccac ccgttgggcg tcgcggcggt gtagagctcg
     8581 atcatggctg gtcctcgcgt gggtgagggc gccagtatag attggcggcg tgccccttct
     8641 catcgatcag tggttgctgg atcactggcg cctcttcgcg gttgccgcgc tgtttttcgc
     8701 gggcgtcatc cttcgccggg cccggtgggc cggcgtccgg gagggacgct ggctgctcaa
     8761 cttcgtgttc tacgtgggcc tgccgatcct gattttcggc gcactgaccg gtgtgccact
     8821 cgaacacgaa cacgccgtcc tgcccgccat agcaatctgc atcacgctcc tgtgctgggg
     8881 cgcggccgct ttcctggcgc gccgtttcgg actgccgaaa aagagcgaag gcgccgtggc
     8941 gctgtgcgcg atgtcgatga acatcgccat gatctatccg ttcgcttcgc tgaagctgag
     9001 caccgcggcg ttttcccagc tcgtcatgtt cgacatgggg catgccgtga tggcctggac
     9061 gctgacgacg atcgtcgcgt gcaagtacgg cgggcacgcc gacgacatcc ccgtactgct
     9121 gcgtcgcgcg ctcgcggccc cgccgttgtg ggtgctgttc ctcgcgctgg cgctgaacct
     9181 cggcggcgcg cccatctcgc gcgacctgct gcacgacgtg ctggtcgtcg gccaatggct
     9241 ggtcctgctg gtgccgctcg cgatgggatt gctggtaagc gccgcgggca tgcgccgtcg
     9301 cgaagtcgtc gccagcgtcg tgcttcgctc cgggctgggc ggactcgcgg gcttcaccct
     9361 ggcatggctg ttcggactcg aaggcaccgc cgtggggatt gcggctctcg cggcgtcggc
     9421 tcccgtggga ttcacggcgg tggtgctttc cgcgcgcgaa ggtctcgatc tcgaactggc
     9481 ggcgagcgcc gcggcgatct ccgtgttcct cgggtcgttg tggctgccgc tggcggtgac
     9541 gttcgtctga tcttcagtgc tggcgaccgc gcgcctacga ccaacggaca agcagtgagg
     9601 catcgcctga caaagaacgc gtaagtcatt gataaaacgt gttgagaatg ccgtcccata
     9661 ccacgccgcc acctctatac tttgcgccgt catgccaatc actcacatgc agcacttcat
     9721 ggtgctcgcc aaggatctcg agaagacgcg
//
LOCUS       DATDEU010000610         4709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1434487, whole genome shotgun sequence.
ACCESSION   DATDEU010000610 DATDEU010000000
VERSION     DATDEU010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434487"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VIV63_14580"
     CDS             <1..1592
                     /locus_tag="VIV63_14580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY6125874.1"
                     /translation="WKKPGVVAVGINDRVPEWHDLWEVDLATGKRVLIEQNATEISSY
                     NLDFDLKPRVASKNDATGTDLLRRVGDKWVKMLRIEQADSLTSGLIGIEFSGKTALLQ
                     TSVGRDKAALERVELATGKTSLIAESKQADLEDVWLEPKTFKPLAYVVNYLKPEITVL
                     DPSVQKDVELLTKELGDGFGIGNRTLDNMFWSVTSDDALAPAVTYLYDRKAGKLTKLF
                     DQRPALAKAPLVPMQSLELKARDGLTLVSYLSLPPGSDKNGDGVPDAPVPMVLNVHGG
                     PWARDGYGFDNEHQWLANRGYAVLSVNYRGSTGFGKSFINASNKEWAAKMHDDLLDAV
                     DWAVKQKVTTKDKVAIYGGSYGGYATLVGLTFTPDTFACGVDIVGPSNLNTLLGSIPP
                     YWKQFFEEFASRVGDPRTEEGKKLLNDRSPISRVDAIKKPLLIGQGANDPRVKQAESD
                     QIVNSMKAKNLPVTYVLYPDEGHGFARPPNRISFYAIAEGFLAQCLGGRYEPVGNDFK
                     GANMKVIEGAQHVSGLTEALK"
     gene            1747..2451
                     /locus_tag="VIV63_14585"
     CDS             1747..2451
                     /locus_tag="VIV63_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125875.1"
                     /translation="MAAVVRNRFYAVIALALAALVLVAFSRTYYLREWFTVPPINLLL
                     HLHSIAFTAWFVLFVIQTRLIAKQNYRTHMQLGIAGLVLAAVVFVLGVATTVDSASAP
                     RLRGGGMNSQQFVFVPLFAITLFAVLVTAAAVLRKHAQIHKRLMILAMLSVLGPPVAR
                     LIVLTQNGNHFLAIQTAVPAAFLIWCLVSDWLKYRIVHPVYAIGGTLLVASWPLRMWI
                     ARTPEWESIGQWMAGM"
     gene            2608..3456
                     /locus_tag="VIV63_14590"
     CDS             2608..3456
                     /locus_tag="VIV63_14590"
                     /inference="COORDINATES: protein motif:HMM:NF042672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6624 domain-containing protein"
                     /protein_id="HEY6125876.1"
                     /translation="MTSGRLNPETFDFAAAGYWQLPQQDRMTWWQAASAALHFGQTNA
                     GDQCSAESQREGASIAWLLLPLQARDSAEWTDAAQAMRKKLATLDAGLAAAAPPPKPG
                     LDPRVSELLARFARDQAVRGVLTQPQWVEGLPPLAANNWSVALISRWTTIDCSNTAWL
                     KTQLAEIHWFDISTYGAEADKAAWHLVQHADREPAFQRRMLQELEALPPGKTDPKRLG
                     YLWDRVALADKRQQRYGTQGQCANGVWKPHDVEDPEHLDARRAKLGMEPIAEHAQLVS
                     REACPK"
     gene            3709..>4709
                     /locus_tag="VIV63_14595"
     CDS             3709..>4709
                     /locus_tag="VIV63_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEY6125877.1"
                     /translation="MSAAAPKELNTYRKYWASRFGTAPFLPMSRAEMDELGWDSCDVI
                     IVTGDAYVDHPSFGMAIMGRLLEAQGFRVGIIAQPDWQSAEPFKQLGKPNLFFGITAG
                     NMDSMVNRYTSDKRIRSDDAYTPGGMGGKRPDRSVVVYAQRAREAFKDVPIVIGGIEA
                     SLRRIAHFDYWSEKVRRSVLLDAKADLLVFGNAERQICEIAHRLAAGEAIAEINDLRG
                     TAFVRKSHDFIEIDSTHIDSPGRLNPPIDPYAMEPEIRKANEEAAAVEAGEAPPPAKE
                     VVVKFARRVKSEDRERSVIRMPSFEMVTADPVLYAHASRILHLEANPGNARAFVQRHG
                     "
BASE COUNT          882 a         1589 c         1423 g          815 t
ORIGIN      
        1 cctggaagaa gcccggcgtc gtcgccgtgg gcatcaacga ccgcgtgccc gaatggcacg
       61 acctatggga agtggacctc gccaccggca agcgcgtcct catcgagcag aacgcgaccg
      121 agatcagcag ctacaacctc gacttcgatc tgaagccgcg cgtcgcttcg aaaaacgacg
      181 cgacgggcac ggacctgctg cgccgcgtag gcgacaagtg ggtcaagatg ctgcgcatcg
      241 agcaggcgga ttcgctgacc agcggactca tcggcatcga attctcgggc aagaccgcgc
      301 tgttgcagac ttccgtgggt cgggacaagg cggcgctcga gcgcgtcgaa ctcgctaccg
      361 gcaagacctc gctgatcgcc gagagcaagc aggcggatct cgaggacgtg tggctcgagc
      421 ccaagacgtt caagccgctg gcctacgtgg tgaactacct caagccggaa atcaccgtgc
      481 tcgatccttc cgtgcagaag gacgtggagt tgctcacgaa agagctgggc gacggcttcg
      541 gcatcggcaa ccgcacactc gacaacatgt tctggtccgt gaccagcgac gacgcgctgg
      601 ctccggccgt gacctatctt tacgaccgca aggccggcaa gttgacgaag cttttcgacc
      661 agcgtcccgc gctggccaag gcgccgctcg tgccgatgca gtcgctcgag ctcaaggcgc
      721 gcgacggtct cacgctggtc tcctatctat cgttgccgcc gggctccgac aagaatggcg
      781 atggtgtccc ggatgcgccg gtgccgatgg tgctgaacgt acacggcggc ccgtgggcgc
      841 gtgacggcta cggcttcgac aacgaacacc agtggctggc caatcgcggt tatgccgtgt
      901 tgtccgtgaa ctaccgcggc tccaccggct tcggcaagag cttcatcaat gcgtccaaca
      961 aggagtgggc cgccaagatg cacgatgacc tgctggacgc cgtcgattgg gcggtgaagc
     1021 agaaggtcac gacgaaggac aaggtcgcca tctacggcgg ttcctatggc ggttatgcca
     1081 cgctggtcgg tctcacgttc acgcccgaca cattcgcgtg tggcgtcgac atcgtcggac
     1141 cgagcaacct caacaccttg ttaggctcca tcccgccgta ctggaagcag ttcttcgaag
     1201 agttcgcgtc gcgcgtcggc gatccgcgta cggaagaggg caagaagctg ctcaacgatc
     1261 gttcgccgat ctcgcgagtg gatgcgatca agaagccgct gctgatcggc cagggcgcca
     1321 acgacccgcg cgtgaagcag gccgagtcgg accagatcgt caactcgatg aaagcgaaga
     1381 acctgcccgt gacctacgtg ctttatccgg atgaaggcca cggtttcgcg cgtccgccga
     1441 accgcatctc gttctacgcc atcgccgaag gcttcctggc gcagtgcctg ggcggccgtt
     1501 acgagcccgt cggcaacgat ttcaagggcg cgaacatgaa ggtgatcgag ggcgcgcagc
     1561 acgtgtccgg cctcaccgaa gcccttaaat aatggggaca ctccccattt cttagcaaaa
     1621 ggggccggag caaccggccc cttttcattt gcgcaacagt ttcgcctgcg cctccaaatc
     1681 attgtccgct gtccagacgg gcggtagttt ccgtataact gagagagaga taaaaacgag
     1741 gcacccatgg ccgcagtggt ccgcaaccgt ttctacgccg tcatcgcgct cgcgctggca
     1801 gcgctcgtgc tcgtagcctt ctcgcgcacc tactacctgc gcgagtggtt cacggtcccg
     1861 ccgatcaacc tgctgcttca cctgcacagc atcgccttca ccgcctggtt cgtgctgttc
     1921 gtcatccaga cgcgattgat cgcgaaacag aactaccgca cgcacatgca gctcggcatc
     1981 gccggcctcg tgctcgccgc ggtggtattc gtgctcggcg tagcgacgac ggtggattcc
     2041 gccagcgcgc ctcgcctgcg tggcggcggc atgaattcgc agcagttcgt gttcgtaccg
     2101 ctgtttgcga tcacgctgtt cgccgtcctc gtgacagccg cggccgtgtt gcgcaagcac
     2161 gcgcagatcc acaagcgcct gatgatcctc gccatgctgt cggtgctggg cccgcccgtc
     2221 gcacgactca tcgtcctcac gcagaacggc aatcacttcc tcgcgatcca gaccgccgtg
     2281 cctgccgcgt tcctcatctg gtgcctggtc agcgactggc tcaagtaccg tatcgtgcat
     2341 cccgtctacg cgatcggcgg cacgctgctc gtcgcctcat ggccgttgcg catgtggata
     2401 gcacgcaccc cggagtggga aagtatcggc cagtggatgg ccggaatgtg acaggagcgc
     2461 ctatactgcc tcggtagcta gactggaaag ggggcgcgcc tgaaaacttc cgcaccgttc
     2521 gtcgctgtcc tattcgcatt cgccgccggc gtcgcctgcg cgaaggagcc acccagcctt
     2581 ccacccccgg ccacgtttgc cgagcaagtg acgtcgggcc gcctcaatcc cgaaaccttc
     2641 gacttcgccg ccgcgggtta ctggcagctt ccacaacagg accgcatgac ctggtggcag
     2701 gctgcgtccg cggcgttgca tttcgggcaa accaacgccg gtgatcaatg ctcggccgaa
     2761 tcccaacgcg agggtgcctc catcgcctgg ctcctgttgc ccttgcaggc cagggattcc
     2821 gccgagtgga ccgacgccgc gcaggcgatg cgcaagaagc tcgcgacgct cgatgccggg
     2881 ctcgcggccg ccgcgcctcc gccgaagccg ggattggatc cgcgcgtgag cgagttgtta
     2941 gcccgcttcg cgcgcgacca ggccgtgcgc ggcgtgctca cacaacccca gtgggtagag
     3001 ggtctcccgc cactcgccgc caacaactgg tccgtcgccc tcatctcgcg ctggaccacc
     3061 atcgattgct ccaacaccgc gtggctcaag acccagctcg cggagattca ctggttcgac
     3121 atctccactt acggcgccga ggccgacaag gccgcgtggc atctcgtgca acacgcggat
     3181 cgcgaacccg ctttccagcg gcgcatgctc caggaactgg aggcgcttcc acccggcaag
     3241 accgatccga aacgcctcgg ctatctatgg gatcgcgttg ccctggccga caagcgccag
     3301 cagcgctacg gcacgcaggg gcagtgcgcc aacggcgtct ggaaacccca cgacgtggaa
     3361 gaccccgaac acctggatgc acgccgcgcc aaactgggca tggaacccat cgccgaacac
     3421 gcacagctcg tttcacgcga ggcgtgcccc aaatagccgc gcgatttcac aattccgggc
     3481 ccaaacagcc ggttttcacg ggtttctttg acattcccgg ccccatccct acactgccgc
     3541 ccatccgacc tctggcagta cgccgcctca aaaacggccg accctcgagg agctcctgaa
     3601 aacaggaaat ggtgttcgcc ggcgtaagtt ccggtgctgt ttcgaacgtt cgcggccacg
     3661 tgccgccagc gcccggactc caacccattt ccgcactgag gttgtcagat gtccgccgcc
     3721 gctcccaaag aactcaacac ctaccgcaag tactgggcca gccgtttcgg caccgccccg
     3781 ttcctgccca tgtcgcgcgc ggagatggac gaactcggct gggattcctg cgacgtcatc
     3841 atcgtcaccg gcgatgcgta cgtggaccac cccagcttcg gcatggccat catggggcgg
     3901 ttgttagagg cccagggctt ccgcgtcggc atcatcgcgc agccggactg gcagagcgcc
     3961 gagcccttca agcagttagg caagcccaac ctgttcttcg gcattaccgc cgggaacatg
     4021 gactcgatgg tgaaccgcta cacctccgac aagcgcatcc gcagcgatga tgcgtacacg
     4081 ccgggcggca tgggcggcaa acgtcccgat cgcagcgtcg tcgtctacgc gcagcgcgcg
     4141 cgcgaagcat tcaaggacgt gcccatcgtc atcggcggca tcgaagcatc cttgcgccgc
     4201 atcgcgcact tcgactactg gagcgagaag gttcgccgct ctgtcttgtt ggacgcgaaa
     4261 gcggaccttt tggtcttcgg aaacgccgag cggcagatct gcgagatcgc gcatcgcctc
     4321 gcggccggcg aagcgatcgc cgagatcaac gacctgcgcg gcactgcgtt cgtgcgcaag
     4381 tcacacgact tcatcgagat cgactccacg cacatcgatt cgcccgggcg cctgaatccg
     4441 cccatcgatc cgtatgcgat ggagcccgag atccgcaagg cgaacgaaga ggccgcggct
     4501 gtcgaagccg gcgaggcgcc gccgccggcg aaggaagtcg tggtcaagtt cgcacgtcgc
     4561 gtgaagagcg aagaccgcga gcgcagcgtc atccgcatgc cgtcgttcga aatggtgacg
     4621 gcggatcctg tgctctacgc gcacgcgtcg cgcatcctgc atctcgaagc caaccccggc
     4681 aacgcacgcg cgttcgtgca gcggcatgg
//
LOCUS       DATDEU010000611         9078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1579698, whole genome shotgun sequence.
ACCESSION   DATDEU010000611 DATDEU010000000
VERSION     DATDEU010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579698"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VIV63_14600"
     CDS             <1..1466
                     /locus_tag="VIV63_14600"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY6125878.1"
                     /translation="IDAVLETGKLCEAAICYSGNLSSPNETKYTLDYYLEMGRKLKAA
                     GTHILGIKDMAGLCQPNAAYTLVKALKQELNLPVHVHTHDTSGIAAASVLAAVAAGCD
                     AVDGAIDAMSGFTSQPNLGSIIEALRYSERDPAIDHGAVRAISMYWEQVRKNYLAFES
                     DMRAGASEVYVHAMPGGQYTNLKEQARSVGLDDSRWPEVSQAYADVNQMFGNIVKVTP
                     SSKVVGDMAILMVSSGLTKEQVIDPAFEVAFPESVVQMMRGDLGRPDGGWPEAIQKKV
                     LKGQKPLDQRPGALMAPLDLVAERKKLDEKLGRSATDTQFASYLMYPKVFLDYAKDRT
                     AYGDLAILPTPVFFYGMEPGDEVSVDIERGKTLIVRFVALSEVRDDGTRQVFFELNGQ
                     PRSIVVTDRSQVAKRPPQRKMEAGNTKHVGAPMPGTIATVKAIVGQKVAKGDLLLTME
                     AMKMETSVRAEADGTVAEVLVKPGVQVDAKDLLVVLS"
     gene            complement(1475..3130)
                     /locus_tag="VIV63_14605"
     CDS             complement(1475..3130)
                     /locus_tag="VIV63_14605"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HEY6125879.1"
                     /translation="MYTLVNIDGVKIVTLLAVALMTALTANAAPPRLEKQGSTQQLIV
                     DGKPFLILGGELGNSSASSAHYMGSHWPRLKAMNLNTVLAPVEWDLIEPSEGKFDWTS
                     VDELLRDARAHDLKLVILWFGAWKNSMSTYVPSWVKRDQKRFPRAQLANGSSVEILSA
                     FSAETRDADVRAFGALMEYLKHVDGDKNTVLMVQVENEIGMLPIARERGAVADKLFAG
                     PVPAELLRALAARGEKLEPELRDRWRKNGAKASGTWTQVFGDDEWGAEVFTAWHYARF
                     VEALVVAGKKRYDIPLYVNVALNRTGRKPGEYPSGGPLPHLIDVWKAGTPTTDMLSPD
                     IYFPNFAQLAARYNRADNMLFIPEANNATNSQGPANAFYSFGELDSVGFSPFSIESSG
                     DAPNALSRSYEVFEQLAPVILANRGKARMAGFRARIEEDGAVIDTPVKKVLGGFEFTV
                     TFIDPWTPKDKQTIADHGGLIVHAGGEDFWFAGQGITVTFKGVGDGPPLAGIDSAYEG
                     VFDEQGQWKPGRALNGDQTHQGRHIRLPPGNPQIQKVRLYRYR"
     gene            complement(3100..4044)
                     /locus_tag="VIV63_14610"
     CDS             complement(3100..4044)
                     /locus_tag="VIV63_14610"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6125880.1"
                     /translation="MDAALNFNQMLAMRVFVRVVEAGTFTNAANSLRMPKPTVTKLVQ
                     GLESHLRIKLLNRTTRRVTVTPDGSAYYERVVRLLGDLEDIESSVTHAKERPRGRLRI
                     DAGAAISTFLILPALPKFLEKYPEIDIECGVSDRPADLIGDNVDCVIRGGPLLEPSLV
                     ARHIGDLRWITCATPAYLEKHGRPTHPSDLESGHRLAGYFFPRSGRMRPLVFQRDGKR
                     IEIPPSPRVAVNDSGAHFVSLRSGLGLGQLLSFMVSCGGATNELESVLKEWQPEAMPV
                     HALYPANRHLSTKVRVFVDWAAELFSQSPCTRLLTSTA"
     gene            4170..4913
                     /locus_tag="VIV63_14615"
     CDS             4170..4913
                     /locus_tag="VIV63_14615"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6125881.1"
                     /translation="MNKKLTGKAALVTGGSRGIGAAIARSLAEDGADVAISYVSTPDK
                     AKALVRELQEKGVRAVAFQADQSKPSEVAQLVNKVAEHFGHLDILVNNAGVFITGPVG
                     QVDDASVERQFAINVGGVNAAVREAAKLMTEGGRIISIGSMAAVTSPWPGISDYSATK
                     AAVAAYTRGWARDLGPKGITVNVVEPGPIDTDMNPASSDSAKPQTAGTALGRFGRPEE
                     VGATVAFLASKNAGFITGASIAVDGGYSA"
     gene            complement(4965..5810)
                     /locus_tag="VIV63_14620"
     CDS             complement(4965..5810)
                     /locus_tag="VIV63_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6125882.1"
                     /translation="MKKIFKWTGFVVAGLAGLGLLGFAYLYVASERELGRQYTLADNA
                     TLVIPTDATEVAEGRRIAQLAGCMQCHGDNLAGTVVDDIPNLVRLVAPNISTRLPNYT
                     DAQLATVLRKGVKPDGHSVLFMPSEMFRHLRDEDLARVIAFLRSKPAVAEGITETTQL
                     RPIGRLIIATGDYKPAARLIESLPAADGSFDANDAVSRGRYLTMNYCSECHAQDLRGV
                     APINAPALAVAKGYSLEQFTRLMQQGIGVGDRQFKMMSPTAQARFTQFAPDEITAVHA
                     FLQSL"
     regulatory      5858..6100
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6201..8120
                     /locus_tag="VIV63_14625"
     CDS             6201..8120
                     /locus_tag="VIV63_14625"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006577.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY6125883.1"
                     /translation="MAPQSVPEARATLARTAQENSRKIISTKSLTPGADTAKSARAAI
                     EVKTDPSRDALLTNFGRATLQDRYLLAGETTQDLFARVARAYGDDAAHAQRLYDYMSK
                     LWFLPATPILSNGGTQRGLPISCFLNSVEDNLDGIVSTWTENVWLASNGGGIGTYWGG
                     VRSIGETVKGCGQTSGIIPFVRVMDSLTLAISQGSLRRGSAAVYLDVHHPEIEEFIEI
                     RKPSGDFNRKSLNLHHGICITDEFMQAVRDDLPFALRSPKNGEALRTLSARHLWQKIL
                     ETRLQTGEPYLLFSDTANNQLSAHQRELGLKVRQSNLCSEILLPTGVDHLGRARTAVC
                     CLSSVNFETWEQWSGEPRFIEDVMRMLDNVLQEFIDHAPASMDTAKYSAMRERSVGLG
                     AMGFHSWLQNNGIPFESALARAANLRMFKHLRREADAASRALAEERGACPDAAERGAQ
                     ERFSHKLAIAPTASISVICGGTSACIEPIPANIYTHKTLSGSFSVRNPALQKVLGVKG
                     LNTEETWQSILAHEGSVQHLEALNEDEKSVFRTAFEIDQRWVIELAADRAPLICQGQS
                     LNLYLRSDIHKWDLLMLHWTAWERGVKSLYYCRSKSVQRAGFAGVEADNTRSWPKKPL
                     DTINDDKYDECLSCQ"
     gene            8111..>9078
                     /locus_tag="VIV63_14630"
     CDS             8111..>9078
                     /locus_tag="VIV63_14630"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF007186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY6125884.1"
                     /translation="MSVITSTQQIELIAKPGLLTPSRSYKPFRYPWAIEYWRRQQQIH
                     WMPEEIPLGEDCKDWNSRITDAERNLLTQVFRFFTQSDVEVQDNYMERYARVFRPTEI
                     KMMLAAFANMETVHIAAYALLLETLGMPDSEFTAFMDYSAMRDKHDYLQGFGVETDAD
                     TLRTLAMFGGFTEGLQLFASFAILLNFPRHNKMRGMGQVVSWSVRDETLHCEGIIRLF
                     HTFAQETGALTQNVKDDIVDCARTVVKLEDRFVDLAFEMGPIEGLSAAEIKAYIRYIA
                     DWRLKQLGLPQVYGVKEHPLPWLTAMLNGVEHSNFFETRATEYAKAA"
BASE COUNT         1738 a         2873 c         2874 g         1593 t
ORIGIN      
        1 ccatcgacgc cgtgctcgag accggcaagt tgtgcgaagc ggccatctgt tattcgggca
       61 atctctcgtc gccgaacgag acgaagtaca cgctcgacta ttacctcgag atgggccgca
      121 agctgaaggc cgccggcaca cacatcctcg gcatcaagga catggccggg ctctgccagc
      181 cgaacgcggc gtacacgctc gtgaaggcgc tcaagcagga gctcaatcta cccgtgcatg
      241 tccacacgca cgatacgtcg ggcatcgccg cggcgagcgt gctcgcggcc gtggccgccg
      301 gctgcgatgc cgtcgacggc gccatcgacg cgatgtcggg tttcacgtcg cagccgaatc
      361 tcggctccat catcgaagcg ctgcgttaca gcgaacggga tccggccatc gaccacggcg
      421 ccgtacgcgc catctccatg tactgggagc aggtgcgcaa gaactacctc gcgttcgaga
      481 gtgacatgcg cgccggcgcg tccgaggtgt acgtgcacgc gatgcccggc gggcaataca
      541 cgaacctcaa ggagcaggcg cgctccgtcg gcctcgacga ttcgcgctgg cccgaagtgt
      601 cgcaggcgta cgccgacgtc aatcagatgt tcggcaacat cgtcaaggtc acgccgtcgt
      661 cgaaggtcgt gggcgacatg gccatcctca tggtgtcgtc gggactcacg aaggaacagg
      721 tcatcgatcc cgctttcgaa gtcgcgttcc cggagtcggt cgtgcagatg atgcgcggtg
      781 acctgggccg ccccgacggc ggctggcccg aagccattca gaagaaggtg ctgaaggggc
      841 agaagccgct cgaccagcgc cctggcgccc tgatggcgcc gctcgatctc gtggcggagc
      901 gaaagaagct cgatgagaag ttaggccgca gcgctaccga cacgcagttc gcgtcctatc
      961 tcatgtatcc caaggtgttc ctcgactacg cgaaagatcg cactgcgtat ggcgacctcg
     1021 ccatcctgcc tacgcccgtt ttcttctacg gcatggagcc gggcgacgaa gtcagcgtcg
     1081 atatcgagcg cggcaagacg ctcatcgtgc ggttcgtcgc gctgtcggaa gtgcgtgatg
     1141 acggcacgcg ccaggtgttc ttcgaactca acggtcagcc gcgttcgatc gtcgtcaccg
     1201 atcgctcgca ggtcgccaag cggccgccgc agcggaagat ggaggcgggt aacaccaagc
     1261 acgtgggcgc gccgatgccg ggcaccatcg cgacggtcaa ggccatcgtc ggtcagaagg
     1321 tggccaaagg cgatctgttg ctcacgatgg aagccatgaa gatggagacc agtgtgcggg
     1381 cggaagccga tggcaccgtc gctgaagtgc tggtgaagcc aggcgtgcag gtggacgcaa
     1441 aggacttgtt ggtcgttctg tcctgaggtc cggatcagcg atagcggtac agccgcactt
     1501 tctggatctg cggatttccc ggcggcaagc gaatgtgccg gccctggtgc gtctggtcgc
     1561 cattgagcgc tcgtcccggc ttccactggc cctgctcatc gaacacgcct tcgtacgctg
     1621 agtcgatgcc ggcgagcggc gggccatcac ccactccttt gaacgtcacg gtgatgccct
     1681 gtccggcaaa ccagaaatct tcaccgcccg cgtgaacaat caggccaccg tgatcggcaa
     1741 tggtttgttt gtctttcggc gtccacggat cgatgaaagt cacggtgaac tcgaaaccac
     1801 ccagcacctt tttcacgggc gtgtcgatga cagcgccgtc ttcctcgatg cgggcacgga
     1861 atcccgccat gcgcgccttg ccgcggttcg cgaggatcac cggcgccagt tgctcgaaga
     1921 cttcgtacga ccgcgacagc gcattcggcg catcgcctga cgactcgatc gaaaacggcg
     1981 agaagcccac cgaatccagt tcgccaaacg agtagaacgc gttcgcaggt ccctggctgt
     2041 tcgtggcgtt gttcgcttcc ggaatgaaca gcatgttgtc ggcgcggttg taacgcgccg
     2101 cgagctgcgc gaagttcggg aagtagatgt ccggcgagag catgtcggtg gtcggcgtgc
     2161 cggccttcca cacatcgatg agatgcggca gcgggccgcc gctcgggtat tcgccgggtt
     2221 tgcggccagt gcggttgagc gcgacgttca cgtacagcgg aatgtcatag cgcttcttgc
     2281 ccgcaacgac gagcgcttcg acgaatcgtg cgtagtgcca cgcggtgaaa acctccgcac
     2341 cccattcgtc gtcgccgaac acctgagtcc aggtgccgga cgctttggcg ccgttcttcc
     2401 gccagcgatc gcgcagctcg ggctccaatt tctcgccgcg agccgcgagc gcgcgcagga
     2461 gttccgcggg cacgggcccc gcgaacagtt tgtcggcgac cgcgccgcgc tcgcgcgcaa
     2521 tcggcaacat gccgatctcg ttctccacct gcaccatgag cacggtgttc ttgtcaccgt
     2581 ccacgtgctt cagatattcc atgagtgcgc cgaacgcgcg tacatcggcg tcgcgcgtct
     2641 ccgcggaaaa cgccgagaga atctccacgc tcgatccatt ggcaagctgc gcgcgcggaa
     2701 aacgcttctg gtcgcgcttc acccaggacg gcacatacgt ggacatgctg tttttccacg
     2761 cgccaaacca caggatcacg agcttgagat cgtgcgcgcg ggcatcgcgc agcagctcgt
     2821 cgacggaggt ccagtcgaac tttccctcgc tgggctcgat gaggtcccat tcaaccggcg
     2881 cgagcacggt gttgaggttc atcgccttca gccgcggcca gtgcgagccc atgtagtggg
     2941 cgctcgacgc gcttgaattg ccgagttcgc cgccgaggat caggaacggt ttgccgtcca
     3001 cgatcagttg ctgcgtgctg ccctgcttct cgagacgggg aggagccgca ttcgcggtga
     3061 gtgccgtcat cagcgcgacg gcgagcagcg ttacgatttt cacgccgtcg atgttaacaa
     3121 gcgtgtacat gggctttgcg agaacagctc ggcggcccag tcgacgaaca cgcgcacttt
     3181 ggtcgacaga tgacgattcg ccggatagag cgcgtgcacg ggcatcgctt cgggctgcca
     3241 ttccttcaac acggattcga gctcgttcgt ggcgccgccg cacgacacca tgaacgacag
     3301 cagttggccc aggccgaggc ccgagcgcag cgacacgaaa tgtgcgccgc tgtcgttcac
     3361 ggccacgcgc ggactcggcg gaatctcgat acgtttgccg tcgcgctgga acaccagcgg
     3421 acgcatccgg ccactgcgcg gaaagaaata ccccgcgagc cggtgtccgg actcgaggtc
     3481 gctcgggtgc gtggggcgac cgtgcttttc caggtacgcc ggcgtcgcgc aggtgatcca
     3541 gcgcagatcg ccgatgtgcc gcgccaccag cgaaggttcg agcagaggac caccacggat
     3601 cacgcagtcg acgttgtcgc cgatgagatc ggccggccga tcgctcacgc cgcactcgat
     3661 gtcgatctcg ggatacttct ccaggaactt cggcagcgcg ggcaggatga ggaacgtgga
     3721 gatcgccgcg cccgcgtcga tgcgcaatcg tccgcgcggg cgctccttcg catgcgtgac
     3781 gctcgactcg atgtcctcga gatcacctaa caacctgacg acgcgctcgt aatacgccga
     3841 gccatcgggc gtgacggtca cgcgccgtgt cgtgcgattg agcagcttga tgcgcagatg
     3901 gctctcgagc ccctggacca gcttggtcac cgtgggcttc ggcatgcgca gggagttggc
     3961 ggcgttggtg aatgtgccgg cttccacgac ccggacgaac acgcgcatgg cgagcatctg
     4021 gttgaaattc agggcggcgt ccatggcggc gattattcac cattaggaat aatgttgtaa
     4081 ctatcggcgt atttatcgct tcctggatag cgaatagcct tctgccaacg cgaaaacacg
     4141 cgccagacac acccactcag gagaaagcca tgaacaagaa actcaccgga aaagccgccc
     4201 tcgtcaccgg cggatcacgc ggcatcggtg ccgcgatcgc ccgttcgctt gccgaggacg
     4261 gcgcggacgt ggccatcagc tacgtcagca ctcccgacaa ggccaaggcg ctcgtgcgcg
     4321 agctgcagga aaaaggcgta cgcgcggtcg cattccaggc ggaccagtca aagcccagcg
     4381 aagtcgcgca gctcgtgaac aaggttgcgg aacacttcgg ccacctcgac atcctcgtga
     4441 acaacgcggg cgtgttcatc accgggcccg tgggccaggt ggacgatgcc tccgtggaac
     4501 ggcagttcgc gatcaacgtg ggcggtgtga acgctgccgt gcgggaagcc gcgaagctca
     4561 tgacggaagg tggccgcatc atctcgatcg gttcgatggc cgcagtcact tcaccctggc
     4621 cgggcatctc tgactactcg gcgacgaaag cggccgtcgc ggcgtacacg cgcggctggg
     4681 cgcgtgacct cgggccgaag ggcatcacgg taaacgttgt cgagccgggt cccatcgaca
     4741 ccgacatgaa tccggcgagc agcgattctg cgaagccgca gacggcgggc accgcgctcg
     4801 gccgcttcgg ccgtccggaa gaagtcggcg cgaccgtcgc gttcctcgcg agcaagaacg
     4861 cgggcttcat caccggcgcc agcatcgcgg tggatggcgg gtacagcgcg tagtcgatcg
     4921 ggtgccgggt ggttgtcgcc cggcatcctc ccgcgtgacg aatatcagag cgactgcagg
     4981 aaggcgtgca cggccgtgat ttcgtccggt gcgaattgcg tgaatcgcgc ctgggctgtc
     5041 ggcgacatca tcttgaactg ccggtcaccc acgccaatgc cttgttgcat gagccgcgtg
     5101 aattgctcga gtgaatatcc cttcgcgact gcgagcgccg gtgcgttgat cggggcaacg
     5161 ccccgcagat cctgcgcatg gcactcgctg caatagttca tcgtgagata gcggccgcga
     5221 ctcacggcat cgttcgcatc gaagcttccg tccgcggcgg gcaacgattc gatcagacgc
     5281 gccgccggct tgtagtcgcc ggtggcgatg atgaggcggc cgatggggcg cagctgtgtc
     5341 gtttcggtga tgccttcagc cacggcaggc ttgctgcgca gaaaagcgat gacgcgtgcg
     5401 aggtcctcgt cccgcagatg ccggaacatt tccgaaggca tgaacaacac gctgtggccg
     5461 tcgggcttca cgccctttcg cagcacggtg gcgagctgcg cgtcggtgta gttcggcaac
     5521 cgcgtgctga tgttcggcgc gacgagacgc accaggttcg gaatgtcgtc gaccacggtg
     5581 cccgcgagat tgtcgccgtg gcactgcatg caaccggcga gctgcgcgat gcgccggcct
     5641 tcagcgacct cggttgcgtc cgtgggaatc acgagcgtgg cgttgtcggc cagcgtgtac
     5701 tggcggccga gctcgcgctc cgacgcgaca tagaggtagg caaaaccgag caacccgagg
     5761 cccgcgagcc cggcgacgac aaatcccgtc cacttgaaaa ttttcttcac gacaatcctt
     5821 ccacactagc ggttgacaaa ttcccgtcgc ggcacacaac atctggtcgt gcatcggttc
     5881 gcgcgtaagc gcgatgaaaa gggaatgcgg tgaaaggatc ttaagtggtc actcaatccg
     5941 cagctgcccc cgcaactgta gaccgcaagc gaaatgtgac tttttcccgc gttatcgtgt
     6001 gaaaaagtgt agccactggg atgcgacgaa tcccgggaag gcctaacaac tcgcgatgga
     6061 gcggtgagcc aggagacctg ccggcgcaca cttcaagact tctgatcgga cggggtgtgc
     6121 cggcagcagc gtcgatgttt ttctgccgct ggcagaattc tcgcgccgct tccgcttccc
     6181 caaagcaaat ggagaagcag atggcacctc aatcagtccc ggaagcgcgc gcgacactcg
     6241 cgcgcacggc ccaggaaaat tcccggaaga tcatcagtac gaaatcgctg acgcccggcg
     6301 cggacacggc gaagtccgca cgcgcggcca tcgaagtgaa gacagatcca tcacgcgatg
     6361 cgttgctcac caactttgga cgcgcgacgc tgcaggatcg ctacctgctg gccggtgaaa
     6421 cgacgcagga cctgttcgca cgcgttgcgc gcgcctacgg tgacgacgcg gcgcatgcgc
     6481 agcggctgta cgactacatg tcgaagctct ggttcctgcc cgccacgccc atcctgtcga
     6541 atggcggcac gcaacgcggc ctgccgattt cctgcttcct gaactccgtc gaagacaacc
     6601 tcgacggcat cgtcagtacg tggaccgaga atgtgtggct ggcgtcgaac ggcggcggca
     6661 tcggtacgta ctggggtggc gtgcgctcca tcggcgaaac ggtgaagggc tgcggccaga
     6721 cttcgggaat cattccgttc gtgcgggtca tggactcgtt gacgctggcg atctcgcagg
     6781 gctcgctgcg ccggggatcc gctgccgtgt atctcgacgt gcaccacccg gaaatcgagg
     6841 agttcatcga gatccgcaaa ccctcgggcg acttcaatcg caagagcctg aacctgcacc
     6901 acggcatctg catcaccgac gaattcatgc aggcggtgcg cgacgatctg ccgtttgcac
     6961 tgcgcagccc gaagaacggc gaggcgttgc gcacgctgtc cgcgcggcat ctgtggcaga
     7021 agattctcga gacacgtctg cagaccggcg agccctatct gctgttcagc gacacggcca
     7081 acaaccagct tagcgcccat cagcgcgagc tcggcctcaa ggtgcgccag tcgaatctct
     7141 gcagcgagat cctgttgccg acgggcgtcg atcacctggg ccgcgcgcgc acggcggtgt
     7201 gctgcctctc ctcggtgaat ttcgagacct gggagcaatg gagcggggag ccgcgcttca
     7261 tcgaagacgt gatgcgcatg ctcgacaacg tgctccagga attcatcgat cacgcgccgg
     7321 cctcgatgga cacggccaaa tattcggcga tgcgcgagcg atcggtcgga ctcggcgcca
     7381 tgggattcca ctcctggctg cagaacaatg gcattccttt cgagagcgcg ctggcgcgag
     7441 ccgcgaacct gcgcatgttc aaacacctgc ggcgcgaagc cgacgcggca tcgcgcgccc
     7501 tcgcggaaga gcgcggtgcc tgccctgacg cggccgagcg tggcgcgcag gagcgcttct
     7561 cgcacaagct cgcgattgcg ccgacggcca gcatttcggt catctgcggc ggcaccagcg
     7621 cctgcatcga gcccattcca gcgaacatct acacgcacaa gacgctgtca ggctccttca
     7681 gtgtccgcaa cccggctctg cagaaagtgt tgggtgtgaa ggggctgaac acggaagaga
     7741 cctggcaatc gatcctcgcg cacgagggtt ccgttcagca cctcgaagca ctgaacgagg
     7801 acgagaagtc ggtgttccgc acggccttcg agatcgatca gcgctgggtc atcgagctcg
     7861 cggccgatcg tgcgccgctc atctgccagg gccagtcgct gaacctctat ctacgcagcg
     7921 acatccacaa gtgggacctg ctcatgttgc actggacggc gtgggaacgc ggcgtgaagt
     7981 cgctgtatta ctgccgcagc aagagcgtgc agcgcgccgg gttcgccggc gtcgaggccg
     8041 acaacacgcg ttcgtggcca aagaaaccgc tcgacacgat caacgacgac aaatacgacg
     8101 agtgcctctc atgtcagtaa tcaccagcac ccagcagatc gaactcatcg cgaagcccgg
     8161 cctgctcacg ccgtcacgca gctacaagcc gttccgttat ccgtgggcga tcgaatactg
     8221 gcggcgccag cagcagatcc actggatgcc ggaagagatt ccgctcggcg aagactgcaa
     8281 ggactggaac tcgcgtatca ccgacgccga acgcaacctg ctcacgcagg tgttccggtt
     8341 cttcacgcaa tccgacgtcg aggtgcagga caactacatg gagcgctacg cgcgcgtgtt
     8401 ccgccccacc gagatcaaga tgatgctggc ggcgttcgcc aacatggaga ccgtgcacat
     8461 cgcggcgtac gcgctgctgc tcgaaacgct gggtatgccc gacagcgagt tcaccgcgtt
     8521 catggactac tccgccatgc gcgacaaaca cgactacctg cagggcttcg gcgtggagac
     8581 ggacgcggac acgctgcgca cgctggccat gttcggcggc ttcaccgaag ggctgcagtt
     8641 gttcgcgagc ttcgcgatcc tgctcaactt cccgcgtcac aacaagatgc gtggcatggg
     8701 gcaggtggtc tcgtggagcg tgcgcgacga gaccctgcat tgcgaaggca tcatccggct
     8761 gttccatacg tttgcgcagg aaaccggcgc gctcacgcag aatgtcaaag acgacatcgt
     8821 cgattgcgca cgtacggtgg tgaaactcga agatcgcttc gtcgatctcg cgttcgagat
     8881 gggcccgatc gaaggactct cggccgccga aatcaaggcc tatatccgct acatcgccga
     8941 ctggcgcctg aagcagttag gcctgccgca ggtctacggc gtgaaggaac acccgcttcc
     9001 atggctcacc gccatgttga atggcgtgga gcactcgaat ttcttcgaaa cccgcgccac
     9061 cgagtacgcg aaggcggc
//
LOCUS       DATDEU010000612         5788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_120621, whole genome shotgun sequence.
ACCESSION   DATDEU010000612 DATDEU010000000
VERSION     DATDEU010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120621"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="VIV63_14635"
     CDS             <1..1542
                     /locus_tag="VIV63_14635"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY6125885.1"
                     /translation="SATRGTITDRYGEPLAVSTPVDSVWVNPKELALASDQLPRLAKA
                     LKRDKNELARRVTSNLDREFLYLARHMQPTEAQKIRALEIPGVYLMREYRRYYPAGEV
                     AGHIIGFTSVDDEGQEGLELAFDHWLAGEDGAKRVIQDRYGKIVQNVEEIRAMRAGRN
                     LVLSIDLRIQYLAYRELKAAMRDQRAKSGSVIVLDVATGEVLAMVNQPAYNPNDRDQL
                     KAKTYRNRAVTDIVEPGSSIKPFVVAAGLASGKFNSRSVIDTNPGYIKVGAKLLEDPH
                     NLGAVDLATILAKSSNVGMTKVALSLEPAQMYGTLTALGFGQVTTSGYPGESAGLLPH
                     YSHWRPVGISSMSRGYGLSITPLQLAHAYATVAALGVSRPISFLRVETPPAGEAALDP
                     GVSRMLIHLLEAVVAPDGGGKQAAIPGYTVAGKTGTAWKATAGGYATDRYLAVFGGIA
                     PASNPRLAAVVIIDEPGAGKYYGGDVAAPVFAGVVGGALRLLAVPPDAPVGDGKEPAR
                     QVAIR"
     gene            1539..3056
                     /locus_tag="VIV63_14640"
     CDS             1539..3056
                     /locus_tag="VIV63_14640"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY6125886.1"
                     /translation="MSATSVATQSLARLLDGIAELPRDVAVTDLTQDSRAARPGGAFL
                     AVRGSAEHGLKYVPQAIANGARAVLWEPAPVAVVPDLPSEILVAPVPRLREHASTIAD
                     RFFGTPSARMAVAGVTGTNGKTTCAYLLAQALEGCGRPAAYLGTIGTGRPGSLVASGL
                     TTGDAITVQRTLAALRADGATSVAMEVSSHALDQARVEAVRFRTAAFTNLTRDHLDYH
                     GSMENYGAAKARLFMREDLVSRVINVDDAFGRQLAIDPRGRGRVIVTSRGHQSHTRAA
                     SGFVRAMHVTLSVRGIELEFDSSWGTGGLVCPLVGDFNVDNLLTVIAVLLDWDLPLEQ
                     VLDTLARVRAAPGRMETFGGTQAPLAVVDYAHTPDALRKALSAARAHCTGRLAVVFGC
                     GGDRDAGKRPLMGTIAAELADDIVITDDNPRTESPQGIAADIARGIPAGKPFRIELDR
                     ARAIRDSLADARPDDVVLIAGKGHEDYQIYGTERRPFSDQQVVAAALTAHTGGGA"
     gene            3053..4423
                     /gene="murF"
                     /locus_tag="VIV63_14645"
     CDS             3053..4423
                     /gene="murF"
                     /locus_tag="VIV63_14645"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY6125887.1"
                     /translation="MSRTLAAFAKSCSGALTGADRAYSGVSTDTRTLKAGELFVALRG
                     PRFNANEFVAAAEKAGAAGAVVDTPVSSPLAQIVVADTQEALTRSAAAWRNGFSMPIV
                     GVAGSNGKTTVKEMTAAILERAGTTLATRGNLNNHIGVPLTLHRLDAKQRFAVVEIGA
                     NRAGEVADLVKLARPTVGLITNAGAEHLEGFGSLEGVARAEGEMVAGLEPSATAVINA
                     DDEFASLWRGMARGQVSTFGVANAADFSARDIHTAIDATGFVTRFELRAPAGVTPVEL
                     HLAGSHNVLNALCAAAAASAAGASLDDVRQGLATMRPVPGRLQFKTAPSGAWIVDDSY
                     NANPSSMKAAIEVLASVDARRWLVVGDMGELGSFADASHGEIGRFARDHHIDRLFATG
                     KLSALAVEAFGSGGEWFPDAESLARAVNSELTREVCVLVKGSRSNRLERVVEALVGSG
                     SSTGSH"
     gene            4428..5513
                     /gene="mraY"
                     /locus_tag="VIV63_14650"
     CDS             4428..5513
                     /gene="mraY"
                     /locus_tag="VIV63_14650"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY6125888.1"
                     /translation="MLYWLAKELTPYYSGFNVFSYLTVRAIFAVITGLALGLLLGPWM
                     IEKLRFGKVGQVVRDDGPKSHFSKAGTPTMGGMLILIAIFASTLLWSDLSNRFVWVVV
                     GVTFTFGLIGFWDDYLKLAKKNPKGLIARYKYSWQSLFGLAAAIFLFYTAKTPAETTL
                     YLPFFKNFAVPMGAAAFIVLSYFMIVGMSNAVNLTDGLDGLAIMPSVMVGGALGVFAY
                     ASGNANFVQYLQIPAIPGAGELIIFCGALVGAGLGFLWFNTYPAQVFMGDIGALALGA
                     ALGTIAVIVRQELVVLIMGGIFVVETASVILQVISFKTTGKRIFRMAPIHHHYELKGW
                     AEPKVIVRFWIITFILVLAGLATLKLR"
     gene            5510..>5788
                     /locus_tag="VIV63_14655"
     CDS             5510..>5788
                     /locus_tag="VIV63_14655"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125889.1"
                     /translation="MTAQASQTARSAVIVGMGRTGLSAARHLQRCGYRVAMTDSRENP
                     PEIAGVKALGASIVTRTGGFDLQLLEQADIVVTSPGVALDDPFFVQARA"
BASE COUNT          929 a         1990 c         1909 g          960 t
ORIGIN      
        1 agcgccaccc gcggcacgat caccgaccgc tatggcgagc ccctggcggt ttccacgccc
       61 gtcgattcgg tgtgggtgaa cccgaaagag ctcgcgctgg ccagcgacca gttgccgcgc
      121 ctcgcgaagg cgctcaagcg cgacaagaac gaactggcgc gacgcgtcac ctctaatctc
      181 gatcgtgagt tcctgtatct cgcgcggcac atgcagccca ccgaggcgca gaagatccgc
      241 gcgctggaga tcccgggcgt gtacctgatg cgcgaatacc ggcgctacta cccggccggc
      301 gaagtcgccg gacacatcat cggtttcacc agcgtcgatg acgagggcca ggaaggactc
      361 gaactcgcgt tcgatcactg gctggccggc gaagatggcg ccaagcgcgt catccaggac
      421 cgctacggca agatcgtgca gaacgtcgaa gagatccgcg cgatgcgcgc cggtcgcaac
      481 ctcgtgttgt cgatcgatct gcgcatccag tacctggcct accgcgagct caaggccgcg
      541 atgcgcgacc agcgcgcgaa gtccggctcg gtgatcgtgc tcgacgtcgc gaccggtgaa
      601 gtgctggcga tggtgaacca gccggcctac aacccgaacg accgcgacca gctcaaggcc
      661 aagacctatc gcaaccgcgc ggtcaccgac atcgtcgagc cgggctcgtc gatcaagcca
      721 ttcgtggtgg cggccgggct cgcttccgga aaattcaatt cgcgcagcgt catcgacacc
      781 aaccccggct acatcaaggt cggcgccaaa ctactcgagg acccgcacaa tctcggcgcc
      841 gtggacctgg cgacgattct cgccaagagc tcgaacgtcg gcatgaccaa ggtggcgctg
      901 tcgctcgagc ccgcgcagat gtacggcacg ctgaccgcgc tcggcttcgg ccaggtgacc
      961 acctcgggtt atccgggcga gtcggccggc ctgctgcctc actactcgca ctggcggccg
     1021 gtcggcatct catcgatgtc gcgcggctac ggcttgtcga tcacgccgct gcagctcgcg
     1081 catgcgtacg cgaccgtggc ggcgctcggc gtgtcccggc ccatctcgtt cctgcgcgtg
     1141 gaaacgccgc cggccggcga ggccgcgctg gatccgggcg tgtcgcgcat gctgatccac
     1201 ctgctcgaag ccgtggtcgc gccggatggc ggcggcaaac aggcggccat tcccggttac
     1261 accgtcgctg gcaaaaccgg caccgcgtgg aaagccacgg cggggggtta cgccaccgac
     1321 cgatacctcg cggtgttcgg cggcatcgcg ccggcgagca acccgcgcct cgcggccgtc
     1381 gtcatcatcg acgagccggg cgccggaaaa tattacggcg gcgatgtggc cgcgccggtg
     1441 ttcgcgggcg tggtgggtgg cgcgttgcgt ctgctcgcgg tgccgcccga tgcgcccgtc
     1501 ggcgacggca aagaacccgc gcgccaggtg gccatccgat gagcgcgacc agcgtggcca
     1561 cgcaaagtct cgccaggctg ctcgacggaa tcgccgagct gccgcgtgac gtcgccgtca
     1621 ccgatctcac gcaggacagt cgtgcggcgc gtcccggcgg cgctttcctc gcggtgcgcg
     1681 gcagcgccga gcacggtctc aaatatgtgc cgcaggcgat cgccaatggc gcgcgtgcgg
     1741 tgttgtggga gccggcgccg gtcgccgtgg tgcccgatct tccgtcggag atcctggtcg
     1801 cgcccgtgcc gcgcctgcgc gagcacgcga gcaccatcgc cgaccgcttc ttcggcacgc
     1861 cttcggcgcg catggccgtt gccggcgtca ccggcaccaa cggcaagacc acctgtgcct
     1921 atctactggc gcaggcgctg gaaggctgcg gccgtccggc cgcctacctg ggcacgatcg
     1981 gcacgggacg cccgggcagt ctcgtggcga gcggactcac caccggtgac gccatcaccg
     2041 tgcaaaggac tctcgccgcg ctgcgcgccg acggcgccac cagcgtggcg atggaagtgt
     2101 cctcgcacgc gctcgaccag gcgcgcgtgg aggcggtgcg ttttcgaacc gccgccttca
     2161 ccaatctcac gcgcgatcat ctcgactatc acggcagcat ggaaaattac ggcgcggcca
     2221 aggcgcgcct cttcatgcgc gaagacctgg tgtcgcgcgt gatcaacgtc gacgacgcct
     2281 ttggccggca actcgccatc gatccgcgcg gccgcggccg agtcatcgtc accagccgcg
     2341 ggcatcagtc gcacacgcgt gcggcatcgg gcttcgtgcg cgccatgcac gtcacgctgt
     2401 cggtccgcgg catcgaactc gaattcgact cgagctgggg tacgggcgga ctggtctgcc
     2461 cgctggtcgg cgacttcaac gtcgacaacc tgctcacggt catcgcggtg ctgctcgact
     2521 gggaccttcc gctcgagcag gtcctcgaca cgctggcgcg cgttcgcgcc gcgccgggcc
     2581 gcatggaaac tttcggcggc acccaggcgc cgctcgcggt ggtcgactac gcacacacgc
     2641 ccgacgcgtt gcgcaaggca ctgtcggcgg cgcgcgcgca ctgcacgggc cggctcgccg
     2701 tggtgttcgg ctgcggcggc gatcgcgacg cggggaagcg tccgctcatg ggaaccatcg
     2761 cggccgaact cgccgacgac atcgtgatca ccgacgacaa tccgcgcacc gaatcgccgc
     2821 agggtatcgc cgccgatatc gccaggggca ttccggcggg caagccgttc cgcatcgaac
     2881 tcgatcgcgc gcgcgccatc cgcgattcgc tcgccgacgc gcgacccgac gacgtagtgc
     2941 tgatcgccgg caaaggccac gaggactacc agatctacgg caccgagcgc aggcccttca
     3001 gcgatcaaca ggtggtcgcg gcggcgctga ccgcgcacac cgggggtggc gcatgagccg
     3061 cacgctcgcc gccttcgcga aatcctgcag tggtgcactc accggcgcgg atcgcgccta
     3121 ttcgggtgtg tccacggata cccgcacgct caaggcgggt gaactgttcg tcgcgttgcg
     3181 cgggccgcgt ttcaatgcga atgagttcgt cgctgcggcc gagaaagccg gtgcggcggg
     3241 agcggttgtc gatacgccgg tttcctcacc gctggcgcag atcgtggtgg ccgacaccca
     3301 ggaagcgctg acgaggtccg ccgccgcgtg gcggaacgga ttctccatgc ccatcgttgg
     3361 cgtggccggc agcaacggca agaccacggt caaggaaatg acggcggcca tactcgagcg
     3421 cgcgggtacc acgctcgcca cgcgcggcaa tctcaacaac cacatcggcg ttccgctcac
     3481 gctgcatcgt ctcgacgcca aacaacgttt cgcggtcgtc gagatcggtg cgaatcgcgc
     3541 cggcgaagtg gcggacctcg tgaaactcgc gcggcccacc gtcggactca tcaccaacgc
     3601 cggcgccgag cacctcgaag gattcggcag cctcgaaggc gtggcgcgcg ccgaaggtga
     3661 aatggtcgcc ggtctcgagc catcggccac ggccgtcatc aacgccgacg acgaattcgc
     3721 cagcctgtgg cgcggcatgg cgcgcggcca ggtgtccacg ttcggcgtcg cgaacgcggc
     3781 ggacttttcc gcccgcgaca tacatacggc catcgacgcg accggcttcg tcacgcgctt
     3841 cgaactgcgt gcgccggctg gcgtgacgcc cgtcgaactg catctggccg gcagtcacaa
     3901 cgtgctgaac gcgttatgcg cggccgcggc ggcttcggcc gccggtgcgt cgctcgacga
     3961 cgtgcgacag ggccttgcca caatgcgccc ggttcctggc cgattgcagt tcaaaaccgc
     4021 gccgagtggc gcctggatcg tcgacgactc ttacaacgcc aacccgagtt ccatgaaggc
     4081 ggcgatcgag gtgctcgcga gcgtggatgc gcggcgctgg ctggtcgtgg gcgacatggg
     4141 cgagctcggc agcttcgccg atgccagcca cggagagatc gggcgattcg cgcgcgatca
     4201 ccacatcgac cggctgttcg ccaccggcaa gctctcggcg ctcgcggtcg aggctttcgg
     4261 ttcgggtggg gaatggttcc cggatgccga gtcgctggcg cgcgccgtca actccgagct
     4321 cacgcgcgag gtgtgcgtgc tggtcaaagg gtcgcgctcc aatcgcctgg agcgcgtggt
     4381 cgaggcgctg gtcggatccg gttcgtcaac aggctcgcac tgaacacatg ctctactggc
     4441 tcgccaaaga actcacgcct tactactcgg gattcaacgt cttctcgtac ctcaccgtgc
     4501 gcgccatctt cgcggtgatc accgggctcg cgctcggcct gctgctcggg ccgtggatga
     4561 tcgagaagtt gaggttcggc aaggtggggc aggtggtgcg cgacgacgga ccgaagtcgc
     4621 atttctccaa ggccggcacg ccgaccatgg gcggcatgct gatactcatc gcgatcttcg
     4681 cgagcacgct tctgtggtcc gacctgtcga accgcttcgt ctgggtggtg gtcggcgtga
     4741 cgttcacgtt cggcctcatc ggtttctggg acgactatct gaagctcgcc aagaagaatc
     4801 cgaagggcct gatcgcgcgt tacaagtatt cctggcagtc gctgttcggc ctggccgccg
     4861 ccatcttcct gttctacacg gcgaagactc cggcggagac cacgctctat ctgccgttct
     4921 tcaagaactt cgccgtgccg atgggcgccg ccgcattcat cgtgctgtcc tatttcatga
     4981 tcgtcggcat gagcaacgcg gtgaatctca ccgacgggct cgatggcctg gcgatcatgc
     5041 cgtccgtcat ggtgggcggt gcgctgggtg tcttcgccta cgcgagcggc aacgccaact
     5101 tcgtgcagta cctgcagatc cccgcgatcc ccggcgccgg cgaactcatc atcttctgtg
     5161 gagcgctggt cggcgcgggc ctcggcttcc tgtggttcaa cacctatccc gcgcaggtct
     5221 tcatgggcga catcggcgcg ctggcgctgg gtgcggcgct cggcacgatt gccgtgatcg
     5281 tgcggcagga actcgtggtg ctcatcatgg gcggcatctt cgtggtcgag accgcgtcgg
     5341 tgatcctgca ggtgatctcg ttcaagacca ccggcaagcg cattttccgc atggcgccga
     5401 tccatcacca ctacgaactc aagggctggg ccgagcccaa ggtcatcgtt cgcttctgga
     5461 tcatcacctt catcctggtg ctcgcgggtc tcgcgacgct caaactccga tgaccgcgca
     5521 agcctcacaa accgcacgca gcgccgtcat cgtcggcatg ggacgcacgg gcctctcggc
     5581 cgcgcgtcac ctgcagcgtt gcggctatcg ggtcgcgatg accgacagcc gcgaaaatcc
     5641 gccggaaatc gccggcgtga aggcgctcgg cgccagcatc gtcacgcgca ccggcggttt
     5701 cgatctgcag cttctcgagc aggccgacat tgtggtgacg tcacccggcg tcgcgctcga
     5761 cgatcccttc ttcgtgcagg cgcgggcc
//
LOCUS       DATDEU010000613         4875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2017717, whole genome shotgun sequence.
ACCESSION   DATDEU010000613 DATDEU010000000
VERSION     DATDEU010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017717"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VIV63_14660"
     CDS             <1..456
                     /locus_tag="VIV63_14660"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125890.1"
                     /translation="DDTGVANSGAVYVFTHSGSSWAQQAYIKASNTGVSDTFGETVAL
                     SDNGNTLAVGAVGESSQTTGINGNQLDNSAVSAGAVYVFTRNVSDWTQRSYLKPNYTS
                     ADDGFGYSLAMSADGNTLAVGLLDDSSTTGVNGNANDDSLDGAGAVYLY"
     gene            626..2551
                     /locus_tag="VIV63_14665"
     CDS             626..2551
                     /locus_tag="VIV63_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY6125891.1"
                     /translation="MPPNPATSPMGANPSASGVTFRLWAPAANAVAVRGTFNAWTDTA
                     LTRGADGTWSAFIPGVTTADQYKFFVTGAGSAGYKRDPYARSLTRIPAFPNSNCEIVA
                     PKSFPWHDAGFRPPPFHSIVLYQLHVGAYYSTDAANLDRRRHQPGRFLDVLFRVEYLA
                     ALGVNAVQLLPIVEFSSERSLGYNGLDYFSPEMDYTVAPDDPDFPRYLAQANALLAKR
                     GLAPYAAADLASEPAQLMALIDLLHVYGIAVIFDVVYNHAGGGFDDESIYFGDRQPTG
                     DNNRSLYFTDQGWAGGLVFAYWKSEVRQFLIDNACFYFDEYHVDGFRSDEVTVIDRYG
                     GWMFLQHLTDTLRFRKPDAIQIAEYWADQSAVLRTRDQGGAGFDAVVASTLREAIRAA
                     LRQATGGAGARVDLGAVASALYPAYGSGWRAVQHLENHDIVRVNNETDREPRVSALAD
                     PSNARSWYARSRSRAANGLLLTAPGIPMLFMGQEFLEDKFWSDSPNFFNDSLIFWDGL
                     SRDRAMQDHLRFCQELIRLRKSLPALTGDSIRVSHVHDDNRIIVFHRWIEGRGEDVLV
                     VVSLRESTWYSYSVGFPRAGAWREVFNSDVYDGWVNPQSAGNGGGIVATDAPLHGFQA
                     SASIVVPANSILVFAAN"
     gene            2634..3497
                     /locus_tag="VIV63_14670"
     CDS             2634..3497
                     /locus_tag="VIV63_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6125892.1"
                     /translation="MSASPHALHPAQQEFPAPPPLDAYATVPLLSGRRMPVLGLGTYE
                     LKHQTVDSVADALESGYHMIDTSGDYHTQRGIGEAIRRSDVARDSIYLVTKIEETADA
                     FEATRANLAELRQDYVDLVLIHRPPERGVGESLWQGLRRAKQAGLTRDIGVSNYSIEQ
                     IEELVYRTGELPVVNQIEWSPFGHSPRMLDFCHDNDIVIQAWSPLTRALRLNDDKLAA
                     MAARYGKQPAQLLIRWNLQLGVVPLPKANHAQHQRENLNVFDFEISQPDMVRLRALNE
                     HYSAMGSLRYV"
     gene            complement(3488..4765)
                     /locus_tag="VIV63_14675"
     CDS             complement(3488..4765)
                     /locus_tag="VIV63_14675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6125893.1"
                     /translation="MIGHRRLFIRSYVVLAGGLLVVAILLDLGFTALQSRQVRAADPW
                     LVSTFQLMESQLATVPQAERAAVAKHFADTLKLDIDVLETADISSAAPLETGPRELED
                     DDGRIYYLWHSEALKGAIRLGPFEPARESLLMRFLPVLFYASILLIVGLWLRPLLRDL
                     RVLTTASQKFATDYREPLDTASRTTQLTSLATNLDDMSARVSQLIQSQKEMTAALSHE
                     MRTPLARVRFAAAVLEGEVDESLRGQLRAVNADVQQIDDLISDMLDYARLDHPGLRMS
                     LQTVPLAPWLRQVLLSCPPHEKKVTTAQQGGIETAWMEPRLMELALSNLLANALRYAK
                     QRVNVTVMRDKDTFRLVVEDDGEGIPESDRTAVFRAFTRLDTSRNRDTGGFGLGLAIV
                     ARIAALHRGRVVAEASSALGGAKLELEWPAVQT"
BASE COUNT          872 a         1575 c         1555 g          873 t
ORIGIN      
        1 gacgataccg gcgtcgctaa ttccggtgcc gtgtacgtct tcactcactc gggcagcagt
       61 tgggcgcaac aggcctatat caaggcttcg aataccggtg tatcggacac attcggtgag
      121 acggtcgcgt tgagtgacaa tggcaacacg ctggctgtcg gcgcagtggg ggaatcgagt
      181 cagaccacgg ggatcaacgg caaccagctc gacaattcgg cggtcagcgc cggggccgtg
      241 tatgtgttca cccgcaacgt cagcgactgg acccagcgca gctatctgaa gcctaactac
      301 acgtccgcgg acgacggctt cggctacagc ctggccatga gtgcggatgg caatacgctc
      361 gcggtaggtc tgctcgacga cagcagcacg acgggcgtca acggcaacgc caatgacgac
      421 agcctcgacg gtgcgggcgc cgtatacctc tattagggca gggtgagcgg agcgaggggc
      481 ggcgcccctc gctccgcact ctttgacgca cgccagtggt gcgacccgca agggacgcgc
      541 ccgatccaat cgcaaacccc gaaatacccc gttgaaagtg agagaaggcg cggtgacgcg
      601 acagtactaa tgataggagt acgtcatgcc acccaatcca gccacttcac cgatgggcgc
      661 caacccttcc gcatcgggtg tcacgttccg gttgtgggcg cccgcggcaa atgctgtcgc
      721 ggtgcgcggt accttcaacg cctggaccga taccgcgctc acgcgaggcg ccgacggcac
      781 ttggtcggcc ttcattccgg gcgtcaccac cgcggatcag tacaagttct tcgtcaccgg
      841 ggccggctcg gcgggataca agcgggatcc ctacgcccgc tcgctcacgc gcataccggc
      901 gttcccgaac tccaattgcg aaatcgtcgc gccgaagtct tttccgtggc acgacgcggg
      961 gtttcgcccg cctccattcc actcgatcgt gctctaccag ctgcacgtcg gggcttacta
     1021 ctcgaccgac gcggccaacc tggatcgccg ccgccatcag ccgggacgat tcctcgacgt
     1081 gctgtttcgt gtcgagtacc tcgccgcgct gggtgtgaat gccgtgcagc tcctgcccat
     1141 cgtggaattc tcgagcgagc gttcactggg ctacaacggc ctcgattact tctcacccga
     1201 aatggactac acggttgcgc cggacgatcc ggattttcca cgctacctcg cgcaggcaaa
     1261 tgcgttgctc gcgaagcgcg gactcgcgcc ttatgcagcc gcggatctgg cgagcgagcc
     1321 tgcgcagctc atggcgctca tcgacctgct gcatgtctac ggaatcgccg tgatcttcga
     1381 cgtggtctac aaccacgcgg gcggcggctt cgacgatgaa agcatctact tcggcgaccg
     1441 gcagcccacc ggcgacaaca atcgaagtct gtatttcacc gaccagggct gggcgggcgg
     1501 actggtgttt gcgtactgga agtcggaggt ccggcagttc ctgatcgaca acgcgtgttt
     1561 ctacttcgat gaatatcacg tcgacggctt tcgctccgac gaggtgacgg tcatcgaccg
     1621 gtacggtggc tggatgtttc tccagcatct gacggacacg ctgcgcttcc gcaagccgga
     1681 cgccatccag attgccgagt attgggccga ccagtcggcc gtcctgcgca cgcgcgacca
     1741 gggcggcgcc ggcttcgatg cggtggtcgc gtccacgttg cgcgaggcca ttcgcgccgc
     1801 gctgcgtcag gcgacgggag gcgcgggcgc cagggtcgac ctgggtgcgg tagctagcgc
     1861 gctctatccg gcgtatggca gcggctggcg cgcggtgcag catctcgaga atcacgacat
     1921 cgtccgcgtg aacaacgaga ccgatcgtga gccgcgcgtg agtgcgctgg ccgatccctc
     1981 gaacgcacgg tcctggtatg cgcgaagccg cagccgggcg gccaacgggt tgttgctcac
     2041 ggcgccggga attcccatgt tgttcatggg ccaggaattt ctcgaagaca aattctggag
     2101 tgacagtccc aacttcttca atgacagcct gatcttctgg gacggcctgt cgcgtgatcg
     2161 cgccatgcag gatcacctgc ggttctgtca ggagctcatc cgtctgcgca aatcgttgcc
     2221 ggcgctgacc ggagattcga tccgtgtttc tcacgtgcac gacgacaacc gcatcatcgt
     2281 ctttcaccgc tggatcgagg gccggggaga ggacgtcttg gtcgtcgtct cgctgcgcga
     2341 atccacgtgg tattcgtact ctgtcggatt cccgcgcgcc ggcgcctggc gcgaagtgtt
     2401 caacagcgac gtgtatgacg gctgggtgaa tccccaatcc gcaggcaatg gcggcggaat
     2461 cgttgccacc gacgcaccgc tgcacggttt tcaggcgtcc gcgtcgatcg tcgtgccggc
     2521 caattcgatc ctcgtatttg ccgcgaactg acgcgcccgg tgtcggtgga ttccgagccg
     2581 ataggtatac acgccgatac ccgaggcggc gttcgccccc tagcatgcac ggcatgtctg
     2641 cctctcccca tgcgctgcac cctgcgcagc aggaattccc cgcgcccccg ccgctcgatg
     2701 cgtacgccac ggtgcctctg ctgagtggcc ggcgcatgcc cgtgttaggg ctcggtacgt
     2761 atgaactcaa gcatcagacc gtcgattcgg tcgcggacgc tctcgagtcc ggctatcaca
     2821 tgatcgacac gtcgggcgac taccacacgc agcgcggcat cggcgaagcg atccggcgca
     2881 gcgacgtggc ccgcgactct atctacctcg tgaccaagat cgaggaaacc gccgatgcct
     2941 tcgaggccac gcgcgcgaac ctggcggagc tgcggcagga ctacgtggat ctcgtgctga
     3001 tccatcggcc acccgagcgc ggagtcggcg aatcgttgtg gcagggcctg cgccgcgcca
     3061 agcaggccgg gctcacgcgc gacatcggtg tcagcaacta ctccatcgag cagatcgagg
     3121 aactggtgta tcgcaccggc gaactgccgg tggtgaatca gatcgagtgg agtccgttcg
     3181 gccacagccc gcgcatgctc gatttctgcc atgacaacga catcgtgatc caggcatgga
     3241 gcccgctcac gcgtgcgctg cgcctcaatg acgacaagct ggccgcgatg gcggcgcgtt
     3301 acggcaaaca acccgcgcag ctattgatcc gctggaacct gcaactgggc gtcgtgccgc
     3361 tgcccaaggc gaatcacgcg caacaccagc gcgaaaatct caacgtcttc gatttcgaga
     3421 tcagccagcc ggacatggtc aggctgcggg cgctcaacga gcattactcg gcgatgggat
     3481 ctttgcgtta cgtctgaacg gccggccact ccagctccaa cttcgcgccg cccagcgctg
     3541 aactggcttc cgcgaccacg cgcccgcgat gaagcgcggc aatgcgcgcc acgatcgcca
     3601 accccaggcc gaatccgccc gtgtcgcgat tgcggctggt atcgagccgc gtgaacgcgc
     3661 ggaagacggc ggtgcgatcg ctctccggaa ttccttcgcc atcgtcttcg accacgaggc
     3721 gaaacgtatc cttgtcgcgc atgaccgtga cgttcacgcg ctgcttcgcg tagcgcagcg
     3781 cgttggccag cagattgctg agcgcgagtt ccatgagccg cggttccatc cacgcggtct
     3841 cgatgccacc ctgctgcgcg gtggtcacct tcttctcgtg aggcgggcag gacagcagca
     3901 cctgccgcag ccagggcgca agcggcacgg tctggaggct catgcgaagc cccggatggt
     3961 cgagccgcgc gtagtcgagc atgtcggaaa tcagatcgtc gatctgctga acgtcggcat
     4021 tcaccgcgcg caattgcccg cgcagcgact cgtcgacttc accttcgagc accgccgccg
     4081 cgaaccgcac gcgcgcgaga ggcgtgcgca tctcgtgtga cagcgcggcc gtcatttcct
     4141 tctgcgactg gatgagctgg ctcacacgcg ccgacatgtc gtcgaggttg gtggcgagac
     4201 tcgtgagctg cgtggtgcgg gatgccgtgt cgagcggctc gcgatagtcg gtcgcgaatt
     4261 tctgcgaggc ggtggtgagc acgcgaaggt cgcgaagcag cgggcgcagc cacaggccga
     4321 cgatcaacag gatcgacgcg tagaacagca ccggcagaaa ccgcatcagc aggctttcgc
     4381 gcgccggctc gaacggcccg agccggatgg cgcccttgag cgcttcggaa tgccacaggt
     4441 agtagatgcg gccgtcgtcg tcttcgagtt cgcgcggacc ggtttcgagc ggcgccgcgc
     4501 tcgagatgtc ggcggtttcg aggacgtcga tatcgagctt cagcgtgtca gcgaaatgtt
     4561 tggcgacggc ggcgcgttcg gcttgcggta cggtggcgag ctgcgactcc atgagctgga
     4621 acgtggacac gagccaggga tccgcagcgc gcacctgacg tgactgcagc gcggtgaacc
     4681 ccaggtccag caggatggcg acgaccagca gtccaccggc gagcacgaca taggaacgga
     4741 tgaacagccg gcgatggcct atcaagtcgc gagttcccag gcgtctggca ccagcaggta
     4801 tcccttgccc cagactgtct tgatgcgggt cggcgccgcg gcgtcatcgc ccagtttgcg
     4861 gcgcagcttg gacac
//
LOCUS       DATDEU010000614         5815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_560857, whole genome shotgun sequence.
ACCESSION   DATDEU010000614 DATDEU010000000
VERSION     DATDEU010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560857"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..794)
                     /locus_tag="VIV63_14680"
     CDS             complement(162..794)
                     /locus_tag="VIV63_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6125894.1"
                     /translation="MTTERVAQLRERANKSLEAFGPAAETAELFKELVRLEPGDALAW
                     FNWGDSLRSIGRFQDAKEALLTARELMPKSRLFAVDTRLGMVFSECGPPAEAEKWFRL
                     ATSNSECHGWVWLLRADNLMRMESFKLARECLKTARVRGNVDLEEVLLNEALIDRCVG
                     DYEDAAHRAKQALEIDPGYDSAKELLASLRGATEARQYVNGLGDDAESRN"
     gene            complement(881..3724)
                     /locus_tag="VIV63_14685"
     CDS             complement(881..3724)
                     /locus_tag="VIV63_14685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6125895.1"
                     /translation="MKTLKKKGKSGKPAHRNSQYEVKAAAGCAIFLMAGSGTALGQDQ
                     AAGAAQPDVIEEVVVTGLRKSIQDSISAKKLDTSITEVVSAEDIGKLPDSSIAESIAR
                     LPGIAAQRTNGRAQTLSIRGLGPDFTVTTLNGREQATTNDNRTVEFDQYPSELVTQVK
                     IYKTPDAGMAYQGIAGTTDISTVHPLSYDSRRMSVTYRREQNAQDANIPGLDDAGNRA
                     NFTYIDQFLDNTLGVAFGVAYNKSPYQAQTKEPWGYADSPTGGGTKIIGGDKSGVQSS
                     FYERTGFLGVIEYRPNDTVHMVLDAYHSDFKELQTIQRVEYGTIWANPSTPVSNPGPV
                     EGNRVQSGTFVDVPFTVIENYNNDRDAKIDSIGLNTDFTITDNWGLNADLSYSKVKRD
                     DLRLESTAGNGKMGDLTFPNVTDDIPFTTGPDGRTYLNPTVNYSDYGVVHLSDPGSWG
                     GGTRRSGFVGHPEINDEIKAIRLTARRTFEGPLSAVSFGVNYADREKSKDPFQSLLYL
                     PGNVSNLPVPEEYRTGVASGSFFGLANGVIGYDAIGLWNSGFWQPIDARYDPNAGDGD
                     RVYDVTNAWTVNEKLTTVYVKFDLDLHLGSLPLRGNIGVQSLTADQSALIGYTPSDIP
                     DNTVDLDVEYRTEGAKYTDILPSLNLALELPHDQKIRFGAAITQARPRMDDLGGGSGF
                     NVTPDSGTPTQGPDGELYYWTSNNGGNPELRPWEANTFDLSWEKYFGDNQGYFSLAAY
                     YKDLTTYIVNETIIVDYTGFGLPSPASDYDLADANRLGAQTAKVNGSGGWIQGFEATL
                     SLPFGIFSQTLDGFGLIVSAAHNDSEIKINDVKSPVPGLSTNVINSTLYFEKAGFSAR
                     VSNRSRDEFVGEVPAFDATLTLNNVAAESILDAQIGYEFRDGPMEGLSVNLQGVNLTD
                     EPFQLTQVGTPSKDLIKYQKYGAIYSLALTYKF"
     gene            complement(3860..5260)
                     /locus_tag="VIV63_14690"
     CDS             complement(3860..5260)
                     /locus_tag="VIV63_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY6125896.1"
                     /translation="MRLKTFVVMTFVAILAAPMASAAETATPVTARQSPEWLRDAVVY
                     EVFPRAFSAEGNFKGVIKQLDRLKELGVTVLWLMPIHPTGKLKAKGALGSPYAVRDYD
                     AVNPEYGTGDDLKQLVAAAHKRGMKVFIDIVINHTSWDSVLIDKHADWYTHDAAGKIV
                     PPNPDWVDVADIDWSKPALREYMTGMLVRWLRDYGIDGFRCDYASGVPTDFWEAVRPE
                     LDRVRPGLAFLAESDDPALLRKAFDIDYAWDFYHAMSETLAGRQPASFVREVWQRAEA
                     KYPRGALRLRFSDNHDQLRTTGQAGLPAALAASAVMYTLDGVPLLYNGMETGDTVESA
                     APALFERAPIQWEMTERRPQVEPYYRALAALRRAHPALTRGSVRWLRNGDEQRVLSYE
                     RAVKGESIVVVVNLSSQSYAGVVETPAGDYRDITPALGSSTTVPSAQQDARRTAMPPA
                     VFLAPWEVRVFQRVTP"
     gene            5565..>5815
                     /locus_tag="VIV63_14695"
     CDS             5565..>5815
                     /locus_tag="VIV63_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017070747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY6125897.1"
                     /translation="MNTAATRLTPPHVAPANPWWRGATIYQIYPRSFLDTNGDGVGDL
                     PGIIERLDYVASLGVEAIWVSPFFKSPMADFGYDIADYRA"
BASE COUNT         1023 a         1832 c         1805 g         1155 t
ORIGIN      
        1 ggttgaaacc cgcgatcgcc ggtatggtcg cttcgcccac gccgacgatg ccgatttccg
       61 acgattccac cagcgtgatg tcgagacgcg gaccgaacac gcgcgccagc gcggccgccg
      121 tcatccagcc ggcggtgcct ccgccggcga tcaccaccga cttaatttcg cgattcagcg
      181 tcgtctccca gtccgttcac gtattgcctc gcctccgtag ctccccgcag cgaagccagc
      241 agttccttgg ccgagtcgta acccgggtcg atctcgagcg cctgtttcgc gcggtgagcg
      301 gcatcttcgt agtcgcccac gcagcgatcg atcagcgctt cgttcagcag cacttcttcc
      361 agatcgacgt tgcctcggac cctggcggtc ttcaggcatt cccgcgcgag cttgaacgac
      421 tccattcgca tgagattgtc ggcgcgcaac agccagaccc agccgtgaca ttcactgttc
      481 gacgtcgcga ggcgaaacca tttttccgct tcggccggag ggccgcattc tgaaaacacc
      541 atgccgaggc gcgtgtccac ggcaaagagt cgagacttgg gcatgagctc gcgcgccgtc
      601 agcaacgcct ccttcgcgtc ctggaaccgc ccgatcgatc gcaacgagtc gccccagttg
      661 aaccacgcca atgcgtcacc gggctcgagc cgcaccagct ccttgaacag ctcggcggtt
      721 tccgcggccg gtccgaatgc ctcgagggac ttgtttgcgc gctcgcgcag ttgcgctacc
      781 cgttcggtgg tcatggaagc attgttccaa gaacggcggc aaaaaaatac ccgccggcat
      841 ttgcgccagc gggtatttca tccggaggaa cggaactcga ctagaacttg tacgtgagag
      901 ccagcgagta gatcgcgccg tatttctggt acttgatgag gtccttcgac ggtgttccga
      961 cctgggtcag ctggaacggc tcatccgtga ggttcacgcc ctgcaggttg acgctcaggc
     1021 cttccatggg gccatcgcgg aactcatagc cgatctgggc atcgaggatg gactccgccg
     1081 cgacgttgtt cagcgtcagc gtcgcgtcga atgccggcac ttcgccgacg aactcgtcgc
     1141 gcgaccgatt gctcacgcgc gccgagaagc cagccttctc gaagtacaac gtcgagttga
     1201 tcacgttcgt cgacagaccg ggcaccggac tcttcacatc gttgatcttg atttcggagt
     1261 cgttatgcgc cgcgctcacg atgagcccga agccgtcgag cgtctgcgag aagatcccga
     1321 aaggcaacga cagcgtcgcc tcgaagccct ggatccagcc gccgctgccg ttcaccttcg
     1381 ccgtctgcgc acccaggcga ttcgcatcgg cgaggtcgta gtcgctcgcg ggcgatggca
     1441 ggccgaatcc cgtgtagtcg acgatgatcg tttcgttgac gatgtaggtc gtcagatcct
     1501 tgtagtaagc cgccagagag aagtaaccct ggttgtcacc gaagtacttt tcccaggaca
     1561 ggtcgaacgt attggcttcc cacggccgca gttccggatt gccgccgttg ttgctggtcc
     1621 agtagtacag ctcgccgtcc ggtccctggg tcggcgttcc ggagtccggg gtcacgttga
     1681 agccggatcc accgcccagg tcgtccatgc gcggacgcgc ctgtgtgatg gccgcgccga
     1741 agcggatctt ctggtcgtgc ggtagttcga gcgcgaggtt caggctcggc aggatgtcgg
     1801 tgtacttggc tccctcggtc ctgtactcga cgtcgaggtc caccgtgttg tcgggaatgt
     1861 cgctcggcgt atacccgatg agggctgact ggtccgccgt cagactctga acgccgatgt
     1921 tgccgcgcaa gggcaatgat cccaagtgca ggtcgagatc gaacttcacg tataccgtgg
     1981 tgagcttctc gttcacggtc caggcattcg taacgtcgta cacgcgatcg ccgtcgcccg
     2041 cattcgggtc gtatcttgcg tcgatgggct gccagaagcc gctgttccac aggccgatcg
     2101 cgtcataacc aatgacgcca ttcgcgaggc cgaagaacga gccgctcgcg acgccggtgc
     2161 ggtattcctc cggcacgggc agattcgaga cgttacccgg cagatacagg agactctgga
     2221 aggggtcctt gctcttttcg cgatcggcgt agttcacacc gaagctcacc gcgctcagcg
     2281 gcccttcgaa cgtccggcga gccgtgagtc gaatcgcctt gatctcgtcg ttgatctccg
     2341 gatgaccgac gaatccggag cggcgcgttc caccacccca ggaaccgggg tcggagagat
     2401 gcaccacgcc gtagtcgctg tagttgaccg tcggattcag gtaggtccgg ccatcgggtc
     2461 cggtggtgaa cgggatgtcg tcggtcacgt tcgggaacgt gaggtcgccc atctttccat
     2521 tgcccgccgt cgactcgaga cgcaggtcgt cgcgcttgac cttcgagtag ctgaggtcgg
     2581 cattgagacc ccagttgtcg gtgatcgtga agtcggtgtt gaggccgatc gaatcgattt
     2641 tcgcgtcgcg atcgttgttg tagttctcga tgacggtgaa cggcacatcg acgaacgtac
     2701 ccgattgcac gcgattgcct tcgacgggac ccgggttcga aaccggcgtc gaaggattcg
     2761 cccagatggt gccgtattcg acacgctgga tggtctgcag ctccttgaag tcggagtgat
     2821 acgcatcgag caccatgtgc acggtgtcgt tcggacggta ttcgatgacg ccgagaaagc
     2881 cggtgcgctc gtagaacgac gattgcacgc cgctcttgtc gccgccgatg atcttcgtgc
     2941 cgccacccgt cggggaatcc gcgtagcccc agggctcctt ggtctgcgcc tggtacgggc
     3001 tcttgttgta agccacgcca aacgcgacac cgagggtgtt gtcgagaaac tggtcgatgt
     3061 aggtgaagtt cgcgcgattg ccggcatcgt cgaggccggg aatgttggcg tcctgcgcat
     3121 tctgttcgcg gcgatacgtg accgacatgc ggcggctgtc gtacgacagc ggatgcacgg
     3181 tcgagatgtc cgtggtgccc gcgatgccct gataggccat gccggcgtcg ggcgtcttgt
     3241 agatcttgac ctgcgtgacg agttccgatg ggtactggtc gaactctacc gtgcggttgt
     3301 cgttggtcgt ggcctgctca cggccgttca gcgtcgtgac ggtgaagtcc gggcccaggc
     3361 cgcggatcga cagtgtctgc gcgcggccgt tggtacgctg cgcggcgatg ccgggcagtc
     3421 gcgcgatcga ttcggcgatg gacgaatccg gcagcttgcc gatgtcttcg gctgacacca
     3481 cttcggtgat ggacgtgtcg agtttcttgg cgctgatcga atcctggatg ctcttgcgaa
     3541 gaccggtgac gaccacctct tcgatcacat cgggctgggc cgctccggcg gcctgatcct
     3601 ggccgagcgc ggtgccgctg ccggccatga gaaaaatggc gcaacccgct gccgccttga
     3661 cctcgtactg cgaattgcgg tgagcaggct ttccgctctt gcctttcttc tttaacgtct
     3721 tcattcgtcc cctctcctaa ctattggttg ttgcatcgca ccgctgcgac cagcgtgcga
     3781 atacgcaaac tttcgtgaga acccctgccg tcaaggatgt tgtcagcgac acacacttgg
     3841 caatcacatg catacgtatt cacggggtaa cccgttggaa aacccttact tcccacggcg
     3901 cgaggaatac ggccggcggc attgccgtgc gccgggcatc ctgttgcgcc gatggcaccg
     3961 tggttgaaga acccagcgcg ggcgtgatgt cgcgatagtc cccggccggc gtctccacga
     4021 ctcccgcgta ggactgcgac gacaggttga ccaccacgac gatggattcg cccttgacgg
     4081 cgcgttcgta gctcaacacc cgctgctcat cgccgttgcg cagccaacgc acggatccgc
     4141 gcgtcagtgc cggatgtgcc cgacgcagcg ccgccagcgc gcggtagtaa ggctccacct
     4201 gcggccggcg ttccgtcatt tcccactgga tgggcgcgcg ctcgaacagc gcgggcgccg
     4261 cggattcgac cgtgtcgccg gtttccatgc cgttgtagag caagggcacg ccgtcgagcg
     4321 tgtacatcac ggccgacgcc gccagcgcgg ccggaagtcc ggcctggccg gtcgtgcgga
     4381 gctggtcgtg attgtccgag aaccgcaggc gcaacgcgcc gcgcggatac ttcgcctccg
     4441 cgcgctgcca cacttcgcgc acgaacgaag caggctggcg tcccgcgagc gtctcactca
     4501 tcgcgtgata gaagtcccac gcgtagtcga tgtcgaaggc cttgcgcaac agcgccgggt
     4561 cgtcggactc ggcgagaaag gcgagacccg ggcgaacgcg gtcgagctcg gggcgcaccg
     4621 cctcccagaa atcggtgggc acgccgctcg cgtagtcgca acggaatccg tcgatgccat
     4681 agtcgcgcaa ccatcgcacc agcattcccg tcatgtattc acgcagcgcc ggcttcgacc
     4741 agtcgatgtc ggcgacgtcc acccagtccg gattcggcgg cacgatcttt ccagccgcgt
     4801 cgtgcgtgta ccagtcagcg tgtttgtcga tcagcaccga atcccacgag gtgtgattga
     4861 tgacgatgtc gatgaacact ttcatgccgc gcttgtgagc ggcggccacg agctgcttga
     4921 gatcatcgcc cgtgccgtat tcgggattga ccgcgtcgta gtcgcgtacg gcgtacgggc
     4981 tgccgagcgc gcccttggcc ttgagttttc ccgtgggatg aatcggcatg agccagagca
     5041 cggtcacgcc gagctctttg aggcggtcga gctgcttgat gacgcccttg aaatttcctt
     5101 cggcggaaaa cgcgcgcggg aatacttcgt atacgacggc gtcgcgaagc cactcgggac
     5161 tctggcgcgc cgtcactggc gtcgcggtct ccgcggcact cgccatcggc gctgcgagaa
     5221 tcgcaacgaa agtcataacg acgaacgtct tcaaacgcat tccgcctccc tcagcggctt
     5281 caggcgacgc gaccatagca gcgcccgcaa gggttaccgt gtgaatacgt atgcacgcgg
     5341 cgtggagaat cggccgccaa aaaagaaggc ctcgcgcgac tgaggggggt caatcgggcg
     5401 aggcctaacg agcctgatat tcacagacat tcgaagttgc gagagcgggt aatggtaggc
     5461 cgcgatttcg cgcgatgacc atgccttgca tacgtattca ggctgaatac gtatgcaaca
     5521 gcaagaacgt ccgaagggtt gcggtaagct cccgcaccgc gagcatgaac acagcagcca
     5581 cacgcctgac cccgccccac gtggcgccgg ccaacccctg gtggcgcggt gccactatct
     5641 accagatcta tccgcgcagc tttctcgaca ccaacggtga tggtgtgggc gacctgcccg
     5701 gcatcatcga acgcctcgat tacgtggcct cgctcggcgt cgaagccatc tgggtatcgc
     5761 cgttcttcaa atcgccgatg gccgacttcg gttatgacat cgcggactac cgcgc
//
LOCUS       DATDEU010000615         8546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2017829, whole genome shotgun sequence.
ACCESSION   DATDEU010000615 DATDEU010000000
VERSION     DATDEU010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017829"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VIV63_14700"
     CDS             complement(<1..1421)
                     /locus_tag="VIV63_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEY6125898.1"
                     /translation="MREPFGHGIRGLMGRGCVLALLFLASFGAREAGAQAITRYVRDT
                     GNINFVTTGGSLRNSATNTCTVNATSTTALSGIPATTTIRNAYLYWGGSGGTADTSVT
                     LNGNSVSATRTFAATYNGVSPSLPYFGAFANVTSIVNTTRNGNYTFGGLNVVTGSPHC
                     DVSAVVSGWSLIVIYESATERLRAINLYDGLASFRGSQVVLTPDGFRVPSANIDGRIA
                     VFTLEGDPANSEQMNGVDEALRYNGFLLDDGINVPGSDPTQQQFDGTINTQGIQTSYG
                     IDVDQYDISARLSPGQTSGTTTYSAGADLVLLMAQIVSATSDPAVDLSVTMSHTGTFV
                     SGGTGTYTITVSNAVSMEREDNTVTVTDTLPAGFTYSSSSGTGWSCGAAGQVVTCTHA
                     PTINPGASLPPLSLVVNVAESAAASVTNSVTVSTPSYELVTANNTATDVTATLDPSVA
                     TSTKTVVDLNGGEASPGDTLRYT"
     gene            complement(1538..2236)
                     /locus_tag="VIV63_14705"
     CDS             complement(1538..2236)
                     /locus_tag="VIV63_14705"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY6125899.1"
                     /translation="MKSANRELVDRLAAEYVLGTLRSRARRRFERWLVSPQVGALVKA
                     WEERLAGLEPRLEPVIPPATVWTGIENRLELRKAHRQPAMRWLAIAASLAFFVMAGVF
                     LLRTPDGVVTPPPPQLTATQKSFLQADPQTIYWRVEILGDNQELSLHVHVVHDLPPGK
                     AHELWALPEGGGTPVSLGLMPHTGDHHRVLTASQRSALAGSRQIAVSLEPEGGSPTGA
                     PTGPVLLVAPIAKA"
     gene            complement(2233..2799)
                     /locus_tag="VIV63_14710"
     CDS             complement(2233..2799)
                     /locus_tag="VIV63_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6125900.1"
                     /translation="MTRTDTLPPEIELAQLLQRCAAQDPAAFRTLYDKTSPILFARLL
                     RMLRRRSIAEEVLQDVYVRIWQRAAQFEAHRGRALAWMVTIARYCAIDLMRRERMTLV
                     SDDAIAEIADESAGDAGGIEKPSNFDSCIEQLNDNTRKCLTLAFVEGRSHDEIARLTT
                     NPLGSVKSWIRRGLLALKECLASERPAT"
     gene            2889..4118
                     /locus_tag="VIV63_14715"
     CDS             2889..4118
                     /locus_tag="VIV63_14715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEY6125901.1"
                     /translation="MPSSRAALTFVLLSVFIDSLGFGIIIPSLPGVIMGLTGEPVSTA
                     ADWSGYLMAVYALLQFFMAPVFGNLSDRFGRRPILLGSLFAFGVDFLLTGLATSMAWL
                     FAGRAFAGVFGASFATAGAYIGDISDDTNRAKNFGLIGAAWGSGFTLGPVIGGFVAEH
                     FDVRAPFFAAAALALANVAFGYFALPESLPVERRRKFEWARANPFGAFKSLTHLPMIA
                     GLLFAVLLYQVAHDSLPAVWMFYTQHKFGWGPSETGWSLTFVGIMTVIVMGGLTGYVV
                     PRLGERRAIVVGFLLMTVGFLGYALVPQGWMIYPAIVFGSLGGIANPAMQSVMSKQAG
                     PSAQGELQGAVASIASIAAIVSPIFMTQLFSHYSAPDAPVQLPGAPYLVSGALVFCCV
                     LIALRTVPKKMGSDPIS"
     gene            complement(4101..4673)
                     /locus_tag="VIV63_14720"
     CDS             complement(4101..4673)
                     /locus_tag="VIV63_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125902.1"
                     /translation="MTELSALWLPILLGAVFAFVASNIIHMVLPWHKNEYPALPDEDK
                     ARAAIGALDVPPGDYMLPRCKSMKEMGSGEYKEKQRLGPNWIITVLPKGDHGMALMMV
                     QWFGFLLLVSFFAAYIASHALAPGAHYHDVFRFIGATAFMGFALAQMPQSIWYRRQWS
                     TTLKLMFDGLIYACIAAGTFGWLWPLGNGV"
     gene            4775..5602
                     /locus_tag="VIV63_14725"
     CDS             4775..5602
                     /locus_tag="VIV63_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEY6125903.1"
                     /translation="MRFARYLPLILIAASLAARADDAAQVQRDLIDAQVARIAIEPQD
                     TPDASGRVFFLGFAGYGEERVFAEEIKLAAQRVGEKYGSLARTVLLLNDRRDLSTWPL
                     ASASSLRYSLKAIAQVMNPDEDILFLALSSHGSPDATIDVSNTGMTSQGLSADTLASL
                     LAESGIRWKVVVVSACFSGAFVKPLADNHTIVITAAAKNRTSFGCSDQRDLTYFGEAF
                     YRDSLPHSTYLRTAFEAARIEVRRREKEIDVRPSQPQAYFGPLMEEKLRGIEQGHGR"
     gene            complement(5590..6063)
                     /locus_tag="VIV63_14730"
     CDS             complement(5590..6063)
                     /locus_tag="VIV63_14730"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY6125904.1"
                     /translation="MKLPIVALSIIAVAAVAASPAEVPYPTNYRSWSHVKSMVIEPGH
                     ALHATFGGIHHLYANADAMAGYRTGRFPDGAVIVFDLLEANAKDHTVTEGARKVVGVM
                     HRDARKYAATGGWGFEGFAGDSHTERAVGANAATACFACHQAKRAQDYVFSTYRP"
     gene            6174..6503
                     /locus_tag="VIV63_14735"
     CDS             6174..6503
                     /locus_tag="VIV63_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6125905.1"
                     /translation="MKTKANKNPRAGVGAARMVEDIVGCKWSLTVLGLIADGVQRPGA
                     MQRRVPGLTAKVLNERLRKLLRFGIIERQIYAEVPPRVEYRLTTFGRRFDGVLKEIAA
                     LDNSSNG"
     gene            6752..>8546
                     /locus_tag="VIV63_14740"
     CDS             6752..>8546
                     /locus_tag="VIV63_14740"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEY6125906.1"
                     /translation="MNSGDWRAYLGAAGVRMANAKRADASWIMRMAPTAMWFAMMGVV
                     LFMLMSKPPLLQLPGLAILLVTFGGLVWVQTWIQRRMSAPMKDGAFLGDVVFEFGGSG
                     FQTRRANTETFNRWSLVNDVTNSSDHVFVWIDAYTAYVVPARDLPVGMTAPAAAVRIQ
                     ELKAAALAAPAEPANSDAPPAAPDTTLTSPVAQNLAPARPSVMQELHALLRLQTWALV
                     DGARLFGRDATILLLGVASVVLWAGLDRLNYEGEVELFVYGLAENAAQLLGILFIAWV
                     ISRLTRPRIELRRGLLLTLGFLPLFVVALWCAGLLPKIGAVTIGVLLAAWGDRYLRAG
                     LRSITGTSQNVAVLAALIATFLLVYLSSHVYYSPGLWFERDADTEQTAATQRANEQLV
                     FEQSTRLNAAIEAMAPREAGTANAFFVGFAGFGPQRVFAEEIGLAAKRIGQRYGSSQR
                     SLLLINDHRNSEKYPLASAPALRHALNAIGKRMNVDEDVLFLSLSSHGDEDATISVAS
                     ELGYWRDLGAPDLADMLRESGIRWKVIVISACHAGSFIGPLHDETTIILTAAAADRAS
                     FGCSDDNDLTYFGEAFYRDALPQAANLRTAFD"
BASE COUNT         1394 a         2701 c         2868 g         1583 t
ORIGIN      
        1 atggtgtagc gcagcgtgtc gcccggcgag gcttcgccac cgttcagatc caccacggtc
       61 ttggtcgacg tcgcgacgct cgggtcgagc gtggcagtga cgtcggtcgc ggtgttgttc
      121 gccgtcacga gttcgtagct gggtgtactc acggtgacgc tgttggtcac ggaggccgcg
      181 gccgattcgg cgacgttcac caccagcgac agtggcggca acgaagcgcc cgggttgatg
      241 gtcggcgcgt gcgtgcaggt cacgacctgg ccagcggcgc cgcagctcca gcccgtgccg
      301 ctcgaggagc tgtaagtgaa gcccgcgggc aacgtgtcgg tcaccgtcac ggtattgtct
      361 tcgcgctcca tcgagaccgc gttcgacacg gtgatggtgt acgtgcccgt gccgccggag
      421 acgaacgtgc cggtgtgcga catcgtcacg ctgaggtcga ccgcggggtc ggaggtggcg
      481 ctcacgatct gcgccatgag cagcacgagg tccgcgccgg ccgagtaggt agtggtgccg
      541 ctggtctggc cgggcgagag ccgggcgctg atgtcgtact ggtcgacgtc gataccgtag
      601 ctggtctgga tgccctgggt gttgatcgtg ccgtcgaact gctgctgcgt cggatcgcta
      661 ccggggacgt tgatgccgtc gtcgagcagg aagccgttgt agcgcagcgc ctcgtcgacg
      721 ccgttcatct gctcggagtt cgcggggtca ccttcgagcg tgaacacggc gatgcggccg
      781 tcgatgttgg cggacggcac gcggaagccg tccggagtca gcaccacctg actgccacgg
      841 aacgacgcga ggccgtcgta caggtttatg gcgcgaagcc gctcggtcgc ggattcgtat
      901 atgacgatga ggctccagcc gctaactacc gcgctgacgt cgcaatgcgg cgagccggtg
      961 acgacgttga gtccaccgaa cgtgtagttg ccgttgcgcg tggtgttgac gatggaggtg
     1021 acgttcgcga acgcgccgaa gtacggcagg ctcgggctga cgccgttgta cgtggccgcg
     1081 aaggttcgcg tggcgctcac ggagttgccg ttgagcgtta ccgacgtgtc cgccgtgccg
     1141 cccgatccgc cccagtacag gtaggcattg cggatcgtcg tggtcgcggg gatgcccgac
     1201 agcgccgtgg tgctggtggc gttgaccgtg caggtgttgg tggcgctgtt gcgcagcgag
     1261 ccgccggtgg tgacgaaatt gatgttgccg gtgtcgcgca cgtagcgcgt gatcgcctgc
     1321 gcgccggcct ctcgcgcgcc gaacgacgcg agaaacagca atgccaaaac acatccgcgg
     1381 cccatcaggc cgcggatgcc gtgaccgaac ggctctctca tgagcgcaat tttcgcagtc
     1441 gcgctgcaac acgggcggga gcagctcaca taatgcggat gcccgtgtgg cgccgatcga
     1501 gaacggcctc acacgggtcc ctgggggtcc tacagcgcta tgccttggcg atcggcgcca
     1561 cgagcagcac cgggccggtc ggcgcgcccg tcggcgaacc gccctcaggc tcgagactga
     1621 ccgcgatctg cctggatcct gcgagcgccg aacgctggga cgcggtgagc acgcgatggt
     1681 gatcgccggt gtgcggcatc aggccgagcg acacgggcgt gccgccgcct tcgggcaacg
     1741 cccacagttc gtgcgccttg ccgggcggca ggtcgtggac cacgtgcacg tgcaggctca
     1801 gctcctggtt gtcgcccagg atctcgacgc gccagtagat agtctgcgga tcggcctgca
     1861 ggaacgactt ctgcgtcgcc gtgagttgcg gcgggggcgg cgtgacgacg ccgtcgggcg
     1921 tgcgcagcag gaacacgccg gccatgacga agaaggcgag cgatgcggca atggcgagcc
     1981 agcgcatggc cggctggcga tgggccttgc ggagctcgag ccggttttcg atgccggtcc
     2041 acacggtggc gggcgggatc accggttcga gacgcggttc caggcccgcg agcctttctt
     2101 cccaagcctt cacgagtgct ccgacctgcg gcgacaccag ccagcgctcg aagcgccggc
     2161 gtgcgcggct acgcagtgtg ccgagtacgt attccgccgc gaggcggtcc accagttcgc
     2221 gattggcgct tttcatgtcg ccggcctttc ggaggccaga cattccttga gcgcgagcag
     2281 gccgcgacgg atccagctct tcacgctgcc cagcggattg gtggtgagcc gggcgatttc
     2341 atcgtgggag cggccttcga cgaaggcgag cgtcaggcac ttgcgtgtgt tgtcgttgag
     2401 ttgctcgatg caggaatcga agttgctggg cttctcgatg ccaccggcat cgcccgcgga
     2461 ttcatcggcg atctcggcga tggcatcgtc ggagaccagc gtcatgcgtt cgcggcgcat
     2521 gaggtcgatg gcgcagtagc gcgcgatggt gaccatccag gcgagcgcgc ggccgcggtg
     2581 cgcttcgaac tgcgcggcgc gctgccagat gcggacgtac acgtcctgca acacctcttc
     2641 ggcgatcgag cggcgccgga gcatgcgcag caggcgcgcg aacaggatgg gcgacgtctt
     2701 gtcgtagagc gtgcggaagg ctgccgggtc ctgcgccgca catcgctgca atagctgggc
     2761 gagctcgatc tcgggcggaa gggtgtcagt ccgggtcatg caggttctac gcgtccgatg
     2821 agaaggtgga tgcagcgcgg cgaaactcta gcacgggacc agacatatgc cggacaatgc
     2881 gggcccgcat gccttcatcc cgcgccgccc tcacgttcgt cctgctgtcc gttttcatcg
     2941 acagcctcgg gttcggaatc atcatcccgt cgctgcccgg cgtcatcatg gggttgaccg
     3001 gtgaacccgt cagcaccgcc gcggactgga gcggctatct gatggcggtg tacgcgctgc
     3061 tgcagttctt catggcgccg gtgttcggca acctgagcga tcgcttcggg cgccggccga
     3121 tcctgctcgg ctctctgttc gcctttggcg tggatttcct gctgacgggc ctggcgactt
     3181 ccatggcctg gctgttcgcc gggcgtgcgt tcgcgggtgt gttcggcgca tcgttcgcga
     3241 cggcgggtgc gtatatcggc gacatcagcg atgacaccaa tcgcgcgaag aacttcgggt
     3301 tgatcggcgc cgcctggggt tccggcttca cgctcggccc tgtgatcggc ggcttcgtgg
     3361 ccgaacactt cgacgtgcgt gcgccgttct tcgcggcggc ggcactggcg ctggccaacg
     3421 tggctttcgg ctactttgcg ttgcccgaat cgctgccggt cgagcggcgc cgcaagttcg
     3481 aatgggcgcg ggcgaatccg ttcggcgcgt tcaagagcct cacccatcta cccatgatcg
     3541 cggggttgct gttcgccgtg ctgctgtacc aggtggcgca cgactcacta cccgccgtgt
     3601 ggatgttcta cacgcagcac aagttcggct ggggcccgtc ggagaccggc tggtcgctga
     3661 ccttcgtcgg catcatgacc gtgatcgtca tgggcggatt gaccggctat gtggtgccca
     3721 ggctgggtga acggcgcgcg attgtcgtgg gctttctgct gatgaccgtc ggcttcctcg
     3781 gctacgcgct ggtgccgcag ggatggatga tctatcccgc catcgtgttc ggttcgctgg
     3841 gcggcatcgc gaatcccgcc atgcagagcg tgatgtcgaa gcaggccggg ccttccgcgc
     3901 agggcgaact gcagggcgcg gtggccagca tcgcgagcat cgccgccatc gtctcgccca
     3961 tcttcatgac gcagcttttc agccactact cggcgcccga cgcaccggtc caactgccgg
     4021 gcgcgcctta tctcgtgtca ggcgcgctgg tgttctgctg tgtgctgatc gcgctgcgca
     4081 ccgtcccgaa gaaaatgggg tcagacccca tttcctagcg gccacagcca accgaaagtg
     4141 ccggcggcga tgcaggcgta gatcaggccg tcgaacatga gcttcaacgt ggtgctccac
     4201 tggcgtcgat accagatgga ctgcggcatc tgcgcgagcg cgaaacccat gaatgcggtg
     4261 gcgccgatga agcggaacac gtcgtggtaa tgcgcaccgg gagccagtgc gtggctggcg
     4321 atgtacgcgg caaagaacga gaccagtaac aggaagccga accactgaac catcatgagc
     4381 gccatgccgt gatcgccctt cggcaaaacg gtgatgatcc agttgggtcc cagccgctgc
     4441 ttttccttgt attccccgga gcccatttcc ttcatgctct tgcagcgcgg caacatgtag
     4501 tcgccggggg gcacgtcgag cgcaccgatc gcggctcgtg ccttgtcctc gtcgggcaac
     4561 gccgggtact cgttcttgtg ccagggcaaa accatgtgga tgatgttgct cgcaacaaag
     4621 gcgaacacgg cgcctaacag gatgggcagc cacagtgcac tgagctcggt catcgatgat
     4681 ctccccgaat tcacgaacgc cgcgcattct gcaaccgttt gcatcggtca acaaccgctt
     4741 gcggccttcc tgaggtcgcg gccacactgc ggcaatgcgc ttcgcccgct atctgccgtt
     4801 gatattgatc gccgcttcgc tggccgcccg cgccgacgac gccgctcagg tgcagcgcga
     4861 tctcatcgat gcacaggtgg cgcgcatcgc catcgaaccg caggacacgc ccgatgcttc
     4921 gggccgcgtg ttcttcctcg gcttcgctgg ctacggcgag gagcgcgtgt tcgccgagga
     4981 gatcaagctg gccgcgcagc gcgtgggcga aaagtacgga tccctggcgc gcaccgtgct
     5041 gctgctcaac gaccggcgcg acctgtcgac ctggcctttg gcgtcggcgt cttcgctgcg
     5101 ttactcgctc aaggctatcg cgcaggtgat gaatcctgac gaggacatcc tcttcctcgc
     5161 gctttcgtca catggatcgc cggacgcgac catcgacgtt tcgaacaccg gcatgacgtc
     5221 gcagggtctg agcgctgata cgctcgcgtc gctgctcgcg gagtccggca tccgctggaa
     5281 agtggtcgtc gtctcggcct gcttctccgg tgcgttcgtc aaaccgctgg ccgacaacca
     5341 caccatcgtc atcaccgccg ccgcgaagaa ccgcacgtcg ttcggctgct cggaccagcg
     5401 cgacctcacc tatttcggcg aggcgttcta tcgcgactcg ctgccacact ccacctatct
     5461 gcgcacggcc ttcgaagccg cgcgcattga agttcgccgg cgcgaaaagg aaatcgacgt
     5521 caggccatcg cagccgcagg cctacttcgg gccgctcatg gaagagaagc ttcgcgggat
     5581 cgagcagggt cacgggcggt aggtgctgaa cacgtagtcc tgtgcgcgtt ttgcctggtg
     5641 acaggcgaaa cacgcagtcg cggcattcgc acccacggct cgctccgtgt ggctgtcgcc
     5701 cgcgaatcct tcgaagcccc agccgccggt ggcggcatat ttccgggcgt cgcggtgcat
     5761 gacgccgacg actttccggg cgccctcggt caccgtgtga tctttcgcat tcgcctcgag
     5821 aagatcgaag acgatgacgg caccatccgg aaacctgccc gtacgataac cggccatcgc
     5881 atccgcattg gcgtagagat ggtggatgcc gccgaaggtg gcatgcaacg cgtgaccggg
     5941 ttcgatgacc atcgacttca cgtgtgacca gctgcggtag ttagtgggat atggaacttc
     6001 ggccggtgat gcggcgacag ccgccacggc gatgatcgag agggcgacga tgggcagttt
     6061 catgcgcgaa ctccgttatg atgcagggca agggacgagc gaggttaggc aggccgtgat
     6121 ccgcgcgtaa gtccgcacca aacggtgggt gggttacttt ccggtgtaga tagatgaaaa
     6181 ccaaggcaaa caaaaatccg cgggccggcg tgggcgcggc gcgcatggtc gaggacatcg
     6241 tcggctgcaa gtggtcgctg acggtgctcg gactgatcgc cgacggcgtg cagcggccag
     6301 gcgccatgca gcggcgcgtg ccggggctga ccgcgaaggt gctcaatgaa cgccttcgca
     6361 agctgctgcg cttcgggatc atcgagcggc agatctacgc ggaagtgccg ccgcgcgtgg
     6421 aataccggct caccacgttc gggcgccggt tcgacggcgt tctcaaggaa atcgcggcgc
     6481 tagacaactc gtcgaacggt tgaggccagt cgaaaaaagg gccagcgccg cagctcgtgt
     6541 tcgcaacgcc ggccccgccg gccacccatc agcgacaacc ggcgaaatat gaacgtcaac
     6601 gtgtcttacg gcggagcatc cgcattggat gcggttgcgt gcggtcggtc actcttcaga
     6661 gcgttcggga gtgaggcagg ccgtctatct gccagacttc agggcgaatc caggtgcagg
     6721 gcgaatggaa agtctcagcg taagaacgtc attgaattcc ggggactggc gcgcctacct
     6781 gggcgccgcc ggcgtccgca tggcgaacgc gaaacgcgcc gacgcaagct ggatcatgcg
     6841 catggcgccg accgccatgt ggtttgcgat gatgggcgtc gtcctcttca tgctgatgtc
     6901 caagcccccg ctgctgcagc tgccagggct cgcgattctg ttggtgacat tcggcggcct
     6961 ggtctgggtg cagacctgga tacagcggcg catgtccgct ccgatgaagg acggcgcgtt
     7021 tctcggtgac gtcgtgttcg aattcggcgg cagcggattc cagacccggc gcgcgaatac
     7081 cgaaaccttc aatcgatggt cgctcgtcaa cgacgtgacg aactcgtcgg atcacgtgtt
     7141 cgtttggatc gatgcttaca cggcctacgt ggtgcccgcg cgcgaccttc ccgtcgggat
     7201 gacggcaccc gcggccgcgg tccgcattca ggagctcaag gccgcggcgc tggcggcacc
     7261 ggccgagccc gcgaattccg acgctccccc ggcagcaccg gacaccacgc tcacctcgcc
     7321 ggttgcccag aacctcgcgc cggcgcgtcc gtccgtgatg caggaactgc acgccctgct
     7381 tcgactgcag acctgggcgc tcgtcgacgg cgcgcgccta ttcgggcgtg acgccacgat
     7441 actgctgctg ggtgtcgcct cggtcgtact ctgggccggt ctcgaccgcc tcaactatga
     7501 gggcgaagta gaactcttcg tctacggtct cgccgagaac gccgcccagt tgctcggaat
     7561 cctgttcatc gcctgggtga tatctcggct cacgcgcccg cgtatcgaac tacgccgggg
     7621 gttgttgctg acactcggat tcctgccact gttcgtggtc gcgctgtggt gcgccggatt
     7681 gctgccgaag atcggcgcgg taacgatcgg cgtcctcctc gccgcgtggg gcgatcgata
     7741 cctgcgtgcc ggcctgcgtt cgataaccgg cacgtcgcag aacgtcgcgg tactcgcggc
     7801 gctgatcgcc acctttctgc tggtctatct atcgtcccac gtgtactact cgccgggact
     7861 ctggttcgag cgcgacgcgg acacggaaca gacggctgcg acgcagcgtg cgaatgagca
     7921 actggtattc gagcagtcga cacgactgaa cgcggcgatc gaagccatgg cccctcgcga
     7981 agccgggacg gccaatgcgt tcttcgtcgg cttcgcgggt tttggcccgc aacgcgtgtt
     8041 cgcggaagag ataggccttg ccgcgaaacg catcggccag cggtacggat ccagccaacg
     8101 atcactgctg ctgatcaacg atcaccgtaa ttcggaaaaa tacccgctgg cgagcgcgcc
     8161 agcgttgcgc cacgccttga acgccatcgg caaacgcatg aacgtcgacg aggacgtgtt
     8221 gttcctgtcg ctgtcgtcgc acggtgacga agacgcgacg atctccgtgg ccagcgaact
     8281 cggctactgg cgcgatctcg gcgcccctga cctcgccgac atgctgcggg agtccggtat
     8341 tcgctggaag gtgatcgtga tttccgcgtg tcacgccgga agtttcatcg gcccgctgca
     8401 cgacgagacg accatcatcc tcacggcagc cgcggccgac cgggcttcgt tcggctgctc
     8461 cgacgacaac gacctcacct acttcggcga agcgttctat cgggatgcgc tgccccaggc
     8521 ggcgaacctg cggaccgctt tcgatg
//
LOCUS       DATDEU010000616         6762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1288792, whole genome shotgun sequence.
ACCESSION   DATDEU010000616 DATDEU010000000
VERSION     DATDEU010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288792"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="VIV63_14745"
     CDS             <1..1707
                     /locus_tag="VIV63_14745"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY6125907.1"
                     /translation="ERLASETTGSDSAEFRLRAARAWLAAGRAADADRVLAQLTPAGL
                     TQQQALEQSLLRIQSLVAQGRGDEAWRQVSAMQAPAAQPAAARYYETRQQVAIATGHL
                     LDGIRSELARERVIGPGDARTARSELLGQLRAAAERGVSLAPPPGSDATVRGWLEAAS
                     VALDNARNPTLGATRLAAFRARYPTHPAVAALAGEPGIGIEEPPASLAAAPHVALMLP
                     LSGRTSAQAGQIRDGFMTAYYELPANTRPRLRVYDTAVSSIADTIAQATTAGAEFIVG
                     PLTREEVVAAGDLLTTRAPILALNFLPPDRPTPQRFYQFALSPEDDARAVARYISASG
                     RRRGVVLTPEGDWGTRVAAAFDEELRAAGGFTLGQASFGTTSRNDFSDSIMQVLRTDD
                     SRTRHGRIQATIGQKLEFEPRRRPDIQFIFAPSQPGAARQLRPQLRFHLAGDIPQYTL
                     GDAYEPHPTANQEIDGVMFPDMPWMLGDEGLSGQVREAARQAFGDAASRRGRLFAFGY
                     DAFRIAASLQRGAGVNPQGLTGTLSIDAEGRVRRELEWARIKNGVPTALDRVDTTAST
                     PP"
     gene            1723..2070
                     /locus_tag="VIV63_14750"
     CDS             1723..2070
                     /locus_tag="VIV63_14750"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY6125908.1"
                     /translation="MDRRQTGTLAENSAAAYLESQGFTIVARNFLRRVGEIDVVARAG
                     DLLVVAEVRTRASDQFGGAAASVGRGKQRRVAATTALFLQRRPDLRNCRVRFDVIIVR
                     DGRVEWLKHAFDA"
     gene            2106..2759
                     /gene="can"
                     /locus_tag="VIV63_14755"
     CDS             2106..2759
                     /gene="can"
                     /locus_tag="VIV63_14755"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEY6125909.1"
                     /translation="MKTLDELFQNNREWAAGILRQDPEFFAKLSRQQTPNYLWIGCAD
                     SRVPANQIVGLLPGDVFVHRNIANMVVHTDLNCLSVMQFAVDILKVKHIIVVGHYGCS
                     GVQAAMRRTRVGLADNWLRHVQDVHEKHQHTLKTFNSEVIASDRLCELNVIEQVQNVC
                     QTSIARDAWERGQELNVHGWIYGIADGLARDLRTTVSDFPEAQPVYQSALAKLAGPR"
     gene            complement(2781..3182)
                     /locus_tag="VIV63_14760"
     CDS             complement(2781..3182)
                     /locus_tag="VIV63_14760"
                     /inference="COORDINATES: protein motif:HMM:NF008769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEY6125910.1"
                     /translation="MNDRARNSKRPYLIRAMHGWMMDNGETPHVIVDAEKPGVEVPRA
                     YVKDGKVVLNLSSTATQRLVMGNDWIEFEARFAGVVHHVRIPVSTVLGIYARETGEGM
                     VFSEQDLGPEPPNKPAPPEDGSRRPQLKVVK"
     gene            complement(3179..3799)
                     /locus_tag="VIV63_14765"
     CDS             complement(3179..3799)
                     /locus_tag="VIV63_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017138266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6125911.1"
                     /translation="MSNRRATMTLFSAPDDLWSHRTRIVIAEKAIEIEIVDVAPGKYP
                     EDLLDLNPYHTTPTLIDRDLVLYDSRVIMEYLDERFPHPPLMPVDPVSRARARLALYQ
                     IEHDWYDLARKMDAPGSKDMAQLRKVLRDNVLGSADLFKLKPFFLSDEFSMVDATIAP
                     ILWRLPRYEIDLPPQAQPILKYASTIFSRPAFRASLSETEREMRLA"
     gene            complement(3854..4624)
                     /locus_tag="VIV63_14770"
     CDS             complement(3854..4624)
                     /locus_tag="VIV63_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY6125912.1"
                     /translation="MPTSKYLVVLALLSVAGAVSANEGGHAAGWQRPADNEIANLPSL
                     QRGARHFMDYCSGCHSIKYMRYSRVAEDLKISDTLRDTYLVKPGDKFSDYINASMPAA
                     DAQEWFGKPPPDLSLVARSRGPDWVFNFLTTFYADPASRQTGVNNLQLPGTAMPHVLA
                     SVQGVQEAVWKGVEHKGEDGTAVVTPQFEKFAPGVVGSLSPEQYDEFARDIVNFLQYV
                     SDPTQVERQSIGIWVVLFLLMFTGIAYMLKKEYWKDVR"
     gene            complement(4614..5858)
                     /locus_tag="VIV63_14775"
     CDS             complement(4614..5858)
                     /locus_tag="VIV63_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014592292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY6125913.1"
                     /translation="MSATIDKSSDGFRAWLNKRLPVDQFMADQLTGYYAPKNFNIWYF
                     FGSLALLVFVMQIVTGIFITMHFKPGEATAFNSVEYLRREVPYGWLLQYMHAVGASFF
                     FIVVYLHMARAFLYGSYKAPREVLWVIGMLIYLALMAEAFMGYVLPYGNMSYWGAQVI
                     VNLFGTIPGIGPDLVIWIRGDYGIGDSTLTRFFSLHVIALPFVIALLILLHLVALRQV
                     GSNNPDGIEIKENLDADGKPKDGIPFHPYYTLKDIVGVGLFLTAFAIVVFFVPTFGGL
                     FLEAPNFEPANPSSTPEHIAPVWYFTPYYAILRAVPDLKMGALLMALSVVAFLFMPWL
                     DRAKNKSMRYRGIASKILLGTFFVTFLMLMWLGLKPADGIYVLLARIFTVLYFAFFIL
                     LPFVSKADGNGPVPDRVTYHAH"
     gene            complement(5858..6481)
                     /gene="petA"
                     /locus_tag="VIV63_14780"
     CDS             complement(5858..6481)
                     /gene="petA"
                     /locus_tag="VIV63_14780"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY6125914.1"
                     /translation="MSDHAADDTVPEVDESRRKFLIAATAGTGAVGAVLTAVPFLASW
                     SPSESARAAGLPTEVDLSKIEPGQMATFFWRKQQIYVVKRTPEMLASLGKLESELKDP
                     KSEKSEQPEYAKNDARAVKPEVLVLIGTCTHLGCLPKTRFTPGDASVMANWPGGFFCP
                     CHGSKFDLAGRVFEGSPASANLKIPPYTFEGDNKLIIGVDTPAKGVA"
BASE COUNT         1240 a         2187 c         2180 g         1155 t
ORIGIN      
        1 gagcggctgg cgtccgagac cacgggcagc gacagcgccg aattccgcct gcgcgcggcg
       61 cgcgcatggc tggccgcagg acgtgcggcc gatgccgatc gcgtgctggc ccagctgacg
      121 cccgccggcc tcacgcaaca acaggcgctc gaacagagcc tgttgcgcat ccagtcgctg
      181 gtcgcccagg gacgcggcga tgaagcctgg cgacaggtaa gcgccatgca ggctcccgcg
      241 gcgcaaccgg cggctgctcg ttattacgaa acgcggcagc aggtggccat cgccaccgga
      301 catctgctcg acggcatccg ctccgaactc gcgcgcgagc gtgtgatcgg ccccggcgac
      361 gcgcgcaccg cgcgctcgga gttgttaggc cagttgcgcg ccgcggccga acgcggggtt
      421 tcgctcgcgc caccgccggg cagcgatgcc accgtgcgcg gctggctcga ggccgcttcg
      481 gtcgcgctcg acaatgctcg caatcccacg ctcggcgcca cacggctggc cgcgtttcgc
      541 gcgcgttacc cgacgcaccc ggcggtcgcc gcgctcgccg gcgaacccgg catcggcatc
      601 gaagagccgc ccgccagtct cgcggcagca cctcacgtcg cgctcatgct gccgttgagc
      661 gggcgcacct ctgcgcaggc gggccagatt cgcgacggct tcatgaccgc ctattacgaa
      721 ctgccggcca acacgcggcc gcgactgcgg gtctacgaca ccgccgtctc gtcgatcgcc
      781 gacaccatcg cccaggccac cacggccggc gctgaattca tcgtgggtcc gctgacgcgc
      841 gaagaagtcg tggccgcggg cgacctgcta actacccgcg cgcccatcct cgcgctcaac
      901 ttcctgccgc cggaccggcc gactccgcag cgcttctacc agttcgcgct ttctccggaa
      961 gacgacgcac gcgcggtggc gcggtacatc agcgccagcg gccgtcgtcg cggcgtcgta
     1021 ctcactcccg aaggcgactg gggcacgcgt gtggccgcgg cgttcgacga agaactgcgt
     1081 gccgccggcg gcttcacgct gggccaggcg agtttcggca cgacgtcgcg caacgatttc
     1141 agcgacagca tcatgcaggt gctgcgcacc gacgacagcc gcacccgtca cggacgcatc
     1201 caggccacca tcggccagaa gctggaattc gagccacgtc ggcgccccga catccagttc
     1261 atcttcgccc ccagccagcc cggtgcggcg cgccagcttc gccctcaatt gcgcttccat
     1321 ctcgccggcg acataccgca atacacgctg ggtgacgcct acgagcctca tccgacggcg
     1381 aaccaggaga tcgacggcgt catgttcccg gacatgccgt ggatgctggg cgacgaaggt
     1441 ttgtcgggcc aggtgcgcga ggccgcgcgc caggctttcg gcgacgccgc gagccggcgt
     1501 ggccgcctct tcgctttcgg atacgacgca ttccgtatcg cggcgtcact gcaacgcggc
     1561 gccggcgtca atccgcaggg tctcaccggc acgttgtcca tcgacgcgga gggacgcgtt
     1621 cgccgtgaac tcgagtgggc gcgcatcaag aatggcgtgc cgactgcact cgatcgtgtc
     1681 gacacaactg cgtcgacgcc gccctagaaa aaaccctccg gcatggatcg ccgacaaaca
     1741 gggacgttgg cggaaaacag tgcggctgcg tacctcgagt cgcagggatt caccatcgtg
     1801 gcgcgaaatt tcctgcgacg tgtcggcgag atcgatgtcg tcgcgcgcgc aggcgatctt
     1861 ctggtggtcg ccgaggtgcg cactcgcgcc agcgatcagt tcggcggcgc cgcggccagt
     1921 gtcggtcgcg gcaagcaacg gcgcgtcgcg gcaaccaccg ccctgttcct gcaacgccgc
     1981 ccggacctgc gcaactgccg cgtcaggttc gacgtgatca tcgtgcgcga tggccgtgtc
     2041 gagtggctga agcacgcgtt tgatgcctag ccaaacgact cgcggacgtt taaggtcgcg
     2101 gccagatgaa aacgctcgac gagttgttcc agaacaatcg cgaatgggcc gcgggcattc
     2161 tccggcagga tcccgagttc ttcgccaagc tttcccgtca gcagacaccc aactatctct
     2221 ggattggttg tgccgacagc cgcgttccgg ccaaccagat cgtcggactg cttcccggcg
     2281 atgtgttcgt ccatcgcaac atcgccaaca tggtggtgca cacggacctg aactgcctgt
     2341 cggtcatgca gttcgcggtc gacatcctca aggtcaaaca catcatcgtc gtgggccact
     2401 acggctgcag cggtgtacag gcggcgatgc ggcgcacgcg cgtcgggctc gccgacaact
     2461 ggctgcggca tgtgcaggac gtgcacgaaa aacaccagca cacgctcaag accttcaaca
     2521 gcgaagtcat cgcgtcggac cggctctgcg aactcaacgt catcgaacag gtgcagaacg
     2581 tctgccagac cagcatcgcg cgcgatgcct gggaacgcgg tcaggaattg aacgtgcacg
     2641 gctggatcta cggcatcgcc gacggactcg cgcgcgatct gcgcaccacg gtttcggatt
     2701 tcccggaagc ccagccggta taccagtcgg cgctggccaa gctcgccggg cctcgctgac
     2761 ccggcgacgc cgggctctaa ctacttgacg accttgagct gcggacggcg gctgccgtct
     2821 tcgggtggcg ctggcttgtt cggcggctcg gggcccaggt cctgttcgga aaacaccatg
     2881 ccctcgccgg tctcgcgcgc gtagataccg agcacggtgc tgacgggaat gcgcacgtgg
     2941 tgcacgacgc ccgcgaagcg cgcttcgaat tcgatccagt cgttgcccat caccagccgc
     3001 tgcgtggcgg tcgaggacag gttgagtacg accttcccat ccttgacgta ggcgcgcggc
     3061 acttccacgc ccggtttctc cgcgtcgacg atgacgtgtg gggtctcgcc gttatccatc
     3121 atccagccgt gcatggcgcg aatcagatat gggcgcttgg aattgcgcgc tcgatcgttc
     3181 atgcgagccg catctcccgc tcggtttccg acaggctggc gcgaaacgcc ggccgcgaaa
     3241 agatggtgct ggcgtacttc aggatgggct gggcctgcgg cggcagatcg atttcgtaac
     3301 gcggcaggcg ccacaggatg ggcgcgatgg tcgcatccac catcgaaaat tcatccgaca
     3361 ggaaaaacgg tttgagcttg aagaggtcgg cgctgcccag aacgttgtcg cgcagcacct
     3421 tgcgaagctg ggccatgtcc ttggatccgg gcgcatccat cttgcgggcc aggtcgtacc
     3481 agtcgtgttc gatctggtag agcgcgaggc gcgcgcgcgc gcgcgacacc ggatcgaccg
     3541 gcatcagcgg cggatgcggg aaacgctcgt cgaggtattc catgatcacg cgcgagtcgt
     3601 acagcacgag gtcgcgatcg atcagcgtcg gcgtcgtgtg atacggattg aggtcgagca
     3661 ggtcctcggg atatttcccg ggcgccacat ccacgatctc gatctcgatc gccttctcgg
     3721 cgataacgat tcgcgtgcga tgactccaca ggtcatcggg cgccgagaac agcgtcatcg
     3781 ttgcacggcg gttagacaat ccgccctcca cgcactacgg cgagttgaag ctctccgatg
     3841 acaatggcgc agctcagcgg acgtccttcc agtattcctt cttcaacatg taggcgatgc
     3901 ccgtgaacat gagaaggaat aacaccaccc agatgccgat gctctggcgt tcgacctgcg
     3961 tcgggtcgct gacgtactgc aggaaattga cgatgtcgcg cgcgaattcg tcgtattgct
     4021 cggggctcag gctgccgacg acgccgggcg cgaacttctc gaactggggc gtcacgacgg
     4081 ccgtaccgtc ttcacccttg tgctccacac ctttccacac ggcttcctgc acaccctgga
     4141 cgctcgcgag cacatgcggc atggccgtgc ccgggagctg caggttgttc acgccggtct
     4201 ggcggctggc cggatcggca taaaacgtgg tcaggaaatt gaacacccag tcggggccgc
     4261 gcgagcgcgc gaccagcgag aggtccggcg gcggcttgcc aaaccattcc tgcgcatcgg
     4321 cggcgggcat cgaggcattg atgtagtcgg agaacttgtc gcccggcttc accaggtagg
     4381 tgtcacgcag cgtgtcggag atcttgagat cttccgcgac tcgcgagtag cgcatgtact
     4441 tgatggaatg acagcccgag cagtaatcca tgaagtggcg cgccccgcgc tgcaacgacg
     4501 gcaggttggc gatttcgttg tccgccggac gctgccagcc ggccgcatgc ccgccttcat
     4561 tcgccgagac cgcgccagcg actgacaaca gcgcgaggac gacgaggtat ttgctagtgg
     4621 gcatggtaag tcactcgatc ggggaccggg ccattgccgt cggccttgga cacgaacggc
     4681 agcaggatga agaacgcgaa gtacagcacc gtgaagatgc gcgcgagcag cacgtagatg
     4741 ccgtcggcgg gcttcagtcc gagccacatg agcatgagga acgtgacgaa gaacgtgccg
     4801 agcaggatct tcgaggcgat gccgcggtag cgcatcgact tgttcttggc gcggtcgagc
     4861 cacggcatga acaggaatgc caccaccgac agggccatga gcaaggcacc cattttcaga
     4921 tccggcacgg cacgcaggat tgcgtagtaa ggcgtgaagt accagaccgg cgcgatgtgc
     4981 tcgggcgtgg acgaaggatt cgccggctcg aagttcggcg cttccaggaa caggccgccg
     5041 aacgtgggca cgaagaacac cacgatggcg aacgccgtca ggaacaggcc gacaccgacg
     5101 atgtccttga gcgtgtagta aggatggaac ggaattccgt ccttcggctt gccgtcggcg
     5161 tccaggtttt ccttgatctc gatgccatcc ggattgttcg agccgacctg acgcagcgcg
     5221 acgaggtgca gcaggatgag cagcgcgatg acgaagggca gcgcgatgac gtgcagcgag
     5281 aagaagcgcg tcagcgtgga atcgccgatg ccgtagtcgc cgcgaatcca gatcacgagg
     5341 tcgggcccga tgcccggaat ggtgccgaac aggttcacga tgacctgcgc gccccagtac
     5401 gacatgttgc cgtagggcag cacgtaaccc atgaaggcct cggccatgag cgccaggtag
     5461 atcagcatgc cgatgaccca caggacttca cgcggcgcct tgtagcttcc gtagaggaag
     5521 gcgcgggcca tgtgcaggta cacgacgatg aagaagaacg acgcgccgac cgcgtgcatg
     5581 tattgcagca gccagccgta gggcacttcg cggcgcaggt actcgaccga attgaacgcg
     5641 gtcgcttcac ccggcttgaa atgcatggtg atgaagatgc ccgtcacgat ctgcatgacg
     5701 aacaccagca gggccagcga tccgaagaag taccagatgt tgaagttctt cggcgcgtag
     5761 tagcccgtga gctggtcggc catgaactgg tcgacgggca gacgtttgtt gagccaggcg
     5821 cggaacccgt cgctgctctt atcaatagtg gcggacatca ggccacccct ttggcaggtg
     5881 tatcgacgcc gatgatgagc ttgttgtcac cttcgaaggt gtatggcgga atcttgagat
     5941 tcgccgaggc cggcgagcct tcgaacaccc ggcccgcgag gtcgaacttg gatccatggc
     6001 aggggcagaa gaagccgccc ggccagttgg ccatgaccga ggcatcaccc ggcgtgaagc
     6061 gcgtcttcgg cagacagccg agatgggtgc aggtgccgat gagcacgaga acttcgggtt
     6121 tgacggcccg ggcatcgttc ttcgcgtatt caggctgctc ggatttctcc gacttcgggt
     6181 ccttgagctc ggactcgagt ttgcccagcg acgccagcat ctcgggcgtg cgcttgacca
     6241 cgtagatctg ctgcttgcgc cagaagaagg ttgccatctg gcccggttcg atcttcgaga
     6301 gatcgacctc ggtgggcaga cccgctgcgc gggcgctctc cgaagggctc cacgatgcca
     6361 ggaaaggcac cgccgtaagc accgcgccaa ccgcgccggt accggcggtg gcagctatca
     6421 gaaacttgcg ccgactctcg tcgacttcgg ggacggtatc gtcggccgcg tgatcgctca
     6481 ttcttgacct caggatcgta cattcgcccc ggcgcgggcg accgcaaaat gcgccgctgc
     6541 gagaccccgg gaggcggcgc attatacaca tgcggatcaa cgctaagacc gcctgccgat
     6601 gaggattatc cggctcatgt cgactcgacc cttcctgcga tttctgacct tcatatcgct
     6661 ctcggcgatc gttggacttg ctgccgcatt cgtcgcggtc atggtgcggc cggagctcat
     6721 cgcccggaac tcgccggcgc cccagccatc gccgcagcct gt
//
LOCUS       DATDEU010000617         2770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1144534, whole genome shotgun sequence.
ACCESSION   DATDEU010000617 DATDEU010000000
VERSION     DATDEU010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144534"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VIV63_14785"
     CDS             complement(<1..806)
                     /locus_tag="VIV63_14785"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY6125915.1"
                     /translation="MNKVLVTLFFAAAGAAGIAPPAFASAREEARLIEASGVLEELRS
                     QRDTLIPDRLMARAYGIAVIPNVVKVAAVVGGRRGSGAMVVRDANGRFSNPIMVSITG
                     GNVGWQIGVQSTDIVLVFTTRKSIEGITDGKLTLGGDASVAAGPVGRSASAATDQNFT
                     AEVYSYSRNRGLFAGVSIDGTVIAIDSKSNNKLYGKSAPAKDIIAGQITTDAEAAKRF
                     QRAIVASTASGSQPAAAPHSAAITTPDAGADAPADSAPPPSGAQTFPMEDA"
     gene            857..1858
                     /locus_tag="VIV63_14790"
     CDS             857..1858
                     /locus_tag="VIV63_14790"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY6125916.1"
                     /translation="MRSKACTYDRLRAIGFRLLFAAACLPAAAHADWITRGENIMGTR
                     CAVELWSEDKARGEAAITSVFDDMKRIDRLMSTWKEDTEISKVNREGGKHPVKISPEL
                     FRLLQVSVEYSELTHGAFDITYASVGYLYDFKKGIHPDQKAIDAALPGINWRHMVLDA
                     RNTTVFFTRPGMRIDLGGIAKGYSVDRGIEILQKQGITRAMVNAGGDTRIIGDRFGKP
                     WVVGVRDPDHEGKVFLRLPLSDTAFSTSGDYERYFDEDGKRFHHIIDPKTGDSARKVR
                     SVTVISGTATRTDALTKSVFIMGPQEGIDFINTLSDVDAVAVAPTGKVIYSKGLAPP"
     gene            1855..>2770
                     /locus_tag="VIV63_14795"
     CDS             1855..>2770
                     /locus_tag="VIV63_14795"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEY6125917.1"
                     /translation="MTAFRSLFVCLALLFASQAEAQSTPTQIHTSPQTDPGVYSGRLK
                     VPYPTPYELATVDSIRGTLEHVHAYVQTAAPVQAINGDTGAPVTDFTRLPPNVALART
                     DLLILTYEWGVTYAGMLRSAQSTGDKRYTDYVNERVTAIATLAAHAKKNLPPGTTHAT
                     YPMPKNGMGMRSILLPGRLDDSGAMCAALIKAELAGLGDKKLRPWIDNYLDFVSNKQF
                     RLKDGTLARNRPLPNSLWLDDLYMSVPCLAQAGKLTGERRYYDDAAKQILQFADRMFV
                     KDKGLFMHAWVQDMQHHPAFHWARANGWA"
BASE COUNT          500 a          912 c          900 g          458 t
ORIGIN      
        1 gcgtcttcca tcgggaaagt ctgcgcaccc gagggcggcg gggcgctgtc ggcgggcgca
       61 tccgcgccgg cgtccggggt ggtgatggcg gcgctatgcg gcgccgcggc cggctgcgag
      121 ccggacgccg tgctggcgac gatggcgcgc tggaagcgct tcgccgcttc ggcgtcggtg
      181 gtgatctggc cggcgatgat gtccttggcc ggcgccgact tgccgtacag cttgttgttc
      241 gacttgctgt cgatggcgat gaccgtgccg tcgatggaca caccggcaaa caacccgcga
      301 ttgcgcgaat acgaatacac ctcggccgtg aagttctgat ccgtggccgc cgacgccgag
      361 cggcccaccg ggccggctgc gaccgaggcg tcgccaccga gcgtgagctt gccgtcggtg
      421 atgccttcga tgctcttgcg cgtggtgaac accagcacga tgtcggtgga ctggacgccg
      481 atctgccatc ccacgttgcc accagtaatg gacaccatga tgggatttga gaaccggccg
      541 ttggcgtcgc gcacgaccat ggcgccgctg ccgcgccgcc cgccgaccac ggccgcgacc
      601 ttcacgacat tggggatgac ggcgattccg taggcccgcg ccatcaggcg atccgggatc
      661 agcgtgtcgc gctggctgcg cagttcttcg agcacgcccg aggcttcgat gagccgggct
      721 tcctcgcggg cgctcgcgaa ggcgggcggt gcgatgccag ccgcgccggc ggcggcgaag
      781 aacaatgtga cgagaacttt attcatgggc actccttgaa ggccgggagc ttaacgcatg
      841 cggcataatc gccaccatgc gaagcaaagc atgtacttac gaccggctgc gcgccatcgg
      901 gttccgcctc ttgtttgccg cggcctgcct gcccgccgcc gcgcacgcgg actggatcac
      961 gcgtggcgag aacatcatgg gcacgcgttg cgccgtggag ttgtggagcg aagacaaggc
     1021 gcgcggtgag gcggcgataa cgtcggtgtt tgacgacatg aagcgcatcg atcgcctcat
     1081 gagcacgtgg aaggaagaca ccgagatctc caaagtgaac cgcgaaggcg gcaaacaccc
     1141 ggtgaagatc agcccggagt tgttcaggct gctgcaggtg tcggtggagt attccgagct
     1201 cacgcacggc gctttcgaca tcacctacgc cagcgtcggc tatctatacg acttcaagaa
     1261 aggcattcac ccggaccaga aggccatcga tgcggcgctg ccgggcatca actggcggca
     1321 catggtgctc gacgcaagga acaccacggt gttcttcacg cgtcccggca tgcgcatcga
     1381 tctcggtggc atcgccaagg gttattcggt ggatcgcggc atcgagatcc tccagaaaca
     1441 gggcatcacg cgcgccatgg tcaacgccgg cggggacacc cgcatcatcg gcgatcgctt
     1501 cggcaagccc tgggtcgtgg gtgtgcgcga tcccgatcac gagggcaagg tattcctgcg
     1561 cctgccgttg tcggacaccg ccttctcgac ctcgggcgac tacgaacgct acttcgacga
     1621 agacggcaaa cggttccacc acatcatcga ccccaagacc ggggattcgg cgcgcaaggt
     1681 tcgcagcgtg acggtgatca gcggcaccgc cacgcgtacc gacgcgctca ccaagagcgt
     1741 gttcatcatg ggcccgcaag agggcatcga tttcatcaac acactttccg acgtcgacgc
     1801 tgtcgcggtc gcgccgacgg ggaaggtgat ctactcaaaa ggactcgcac cgccatgacc
     1861 gcattccgtt cgctgttcgt ctgcctcgcc ctgctgtttg ccagccaggc cgaggcgcag
     1921 tcgacgccta cgcagatcca tacctcgccg cagacggatc ccggtgtcta ttcgggccgt
     1981 ctcaaggtgc cgtatcccac cccttacgaa ctcgcgacgg tggattcgat ccgcggcacg
     2041 ctcgagcacg tccacgcgta cgtgcaaacg gccgccccgg tgcaggccat caacggcgat
     2101 acgggcgcgc ccgtcacgga cttcaccagg ctgccgccga atgtcgcgct cgcgcgcacc
     2161 gacctgctga tcctgacgta cgagtggggc gtcacctatg ccggcatgtt gcgcagtgcg
     2221 cagtccaccg gcgacaagcg ttacacggac tacgtgaacg aacgcgtcac ggcgatcgcg
     2281 acgctggccg cgcacgcgaa gaagaacctg ccgccgggca ccacgcacgc tacctacccg
     2341 atgccgaaga acggcatggg gatgaggtcc atcctgctgc cgggcaggct cgacgattcc
     2401 ggcgcgatgt gcgcggcgct catcaaggcg gagctcgccg gcctcggcga caagaagctg
     2461 cggccgtgga tcgacaacta cctcgacttc gtgtccaaca agcagttccg gctcaaggac
     2521 ggcacgctgg cgcgcaaccg cccgttgccg aattcgctgt ggctcgacga cctctacatg
     2581 agcgtgccct gcctggcgca ggcggggaag ctcaccggcg agcgtcgcta ctacgacgac
     2641 gcggcgaaac agatcctgca gttcgcggac cgcatgttcg tcaaggacaa gggcctgttc
     2701 atgcatgcct gggtccagga catgcagcac cacccggctt ttcactgggc gcgcgccaat
     2761 ggctgggcca
//
LOCUS       DATDEU010000618         2846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2018177, whole genome shotgun sequence.
ACCESSION   DATDEU010000618 DATDEU010000000
VERSION     DATDEU010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018177"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..560
                     /locus_tag="VIV63_14800"
     CDS             51..560
                     /locus_tag="VIV63_14800"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6125918.1"
                     /translation="MSARTLKGLAKGLALAAVFVLPAFAASSSGPAPGFQLSARGGKS
                     IDLSSLKGQVVMINFWATWCGPCRQEMPLLEDIYKKYKPMGFTMLAVNVEPDSKAAEA
                     WLGKLSKPVTFPVAFDTESKVSKMYKVAGMPSTVFVDRKGNIRVMHKGYKPGDENFYL
                     TQIRSMLKE"
     gene            579..806
                     /locus_tag="VIV63_14805"
     CDS             579..806
                     /locus_tag="VIV63_14805"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEY6125919.1"
                     /translation="MRIRGLFVLSALAVLSAGCSSIEPWVKPYDRENLADPIMTFSRA
                     PISQDFLDHVFESREGSRGATGGGGGGCGCN"
     gene            822..2015
                     /locus_tag="VIV63_14810"
     CDS             822..2015
                     /locus_tag="VIV63_14810"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEY6125920.1"
                     /translation="MKALQTRLVVFAITCFVGAAHAGVLPEDRADLLYFRYDGGGVEI
                     TGPSVLVRKSIGEHVSVAANYYIDMVSSASIDVETTASPYEDERTQGSLSMDFLHGKS
                     TYSIGYVTSDESDYTAKTMFAAVSHDMFGDLTTISFSYKEGENEVFRNVFIDDVKVND
                     PGFAEEMNSKSFSVSLSQILTKNMILSGQYEVITDEGWLNSPYRQVRFFVNGDPNIQD
                     TQPEIYPSTRSSNAASIRTKYFLPYRAAVDVMYRFYTDTWGVVGHTGELGYVHPVGSH
                     WIFEARGRYYTQTKADFYEDIFPYADYSNFMARDKELATYNAFTAGLTATYEFKIERF
                     PWLSKGALSLRYDYMTVNYDDFRDARYSIGVFGQPPADPLQPGTEPLYKLNANIMQFY
                     ISAYF"
     gene            2125..>2846
                     /locus_tag="VIV63_14815"
     CDS             2125..>2846
                     /locus_tag="VIV63_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125921.1"
                     /translation="MDLPRTRGKVARIVALGVIASAAVVYVARDGSEPPREGMRAEAS
                     LSIEQPAAVQPSTRATRTPESRGVDTPSVPLVELERQLAASDPADRELVLEAELPAMV
                     AKNPEELARFAELQTDPRLRELLIRQVAQEWAQTDLDRAMAWANSLPDSPERVATLID
                     LSLTVAQTDPQRAVNMREPQVGNIEPDGVLENLVQQWALQDFDAALAWANARPHNAQR
                     DKLLQRLVYARASAGQPAEAAKL"
BASE COUNT          564 a          946 c          837 g          499 t
ORIGIN      
        1 catcgaaacc ctgttgagcg cgaccgtctt cttctgacgg agtgatcatc atgtcggcac
       61 gcacactgaa aggactcgct aaaggactcg cgctcgcggc ggtattcgtg ttgccggcgt
      121 ttgccgcatc gtcctcgggc cctgctccgg gcttccagtt gtccgcccgc ggcggcaagt
      181 cgatcgacct ctcgtcgctc aaaggccagg tcgtcatgat caacttctgg gccacgtggt
      241 gtggtccgtg ccggcaggaa atgccgctgc tcgaagacat ctacaagaag tacaagccca
      301 tgggcttcac catgctcgcg gtcaacgtcg aacccgactc aaaggctgcc gaagcctggc
      361 tcggcaaact cagcaagccg gtgacgttcc cggtggcgtt cgacaccgag agcaaggtca
      421 gcaagatgta caaggtggcc ggcatgccga gcaccgtgtt cgtcgatcgc aaaggcaaca
      481 tccgggtcat gcacaagggc tacaagcccg gcgatgagaa cttctatctc acgcagatcc
      541 ggtcgatgct caaagaatag gatcaaggag agaaggtcat gcgcatccgt ggtttgtttg
      601 tcctgtccgc ccttgccgtg ttgtcggcag gctgcagctc gatcgagccg tgggtgaaac
      661 cgtacgatcg cgagaatctc gccgatccga tcatgacctt ctcgcgcgcg cccatctcgc
      721 aggacttcct cgaccacgtg ttcgaatcgc gcgaaggttc gcgcggcgcc accggcggcg
      781 gtggcggcgg ctgcggttgt aactagcaaa gcagccgagt gatgaaagcc ctacagacac
      841 gtctcgtcgt attcgccata acctgcttcg tgggcgcggc tcacgccggc gtgcttcccg
      901 aagaccgcgc cgacctcctc tacttccgtt acgacggcgg tggcgtggag atcaccggcc
      961 cctcggtgct ggtgcgcaag agcatcggcg aacacgtctc cgtggccgcc aactactaca
     1021 tcgacatggt gtcgtcggcg tccatcgacg tggagaccac ggcgagcccg tacgaagacg
     1081 aacgcacgca gggcagcctg tccatggatt tcctgcacgg aaaatccacg tacagcatcg
     1141 gctacgtgac cagcgacgag agcgactaca cggccaagac catgttcgcg gccgtgagcc
     1201 acgacatgtt cggcgacctc accaccatct cgttcagtta caaggaaggc gagaacgagg
     1261 tgttccgcaa cgtcttcatc gacgacgtca aggtcaacga cccgggcttc gccgaggaga
     1321 tgaactccaa gagcttcagc gtcagcctct cgcagatcct caccaagaac atgatcctct
     1381 cgggtcagta cgaagtgatc accgacgaag gctggctcaa cagcccctat cgccaggtgc
     1441 gtttcttcgt gaacggcgac ccgaacatcc aggacacgca gcccgagatc tacccgagca
     1501 cgcgctcgag caacgccgcg tcgatccgca ccaaatattt cctgccgtat cgcgccgccg
     1561 tcgacgtcat gtaccgcttc tacaccgaca cctggggtgt cgtgggccac accggcgagc
     1621 tcggctacgt gcatccggtg ggcagccact ggatcttcga agcccgcggg cgttactaca
     1681 cgcagaccaa ggccgacttc tacgaagaca tcttcccgta cgccgactac tccaatttca
     1741 tggcgcgcga caaagagctc gccacgtaca acgcgttcac ggccggcctc actgccacct
     1801 acgaattcaa gatcgaacgt ttcccctggc tgtccaaggg cgcgttgagc ctgcgatacg
     1861 actacatgac ggtgaactac gacgacttcc gcgatgcgcg ctacagcatt ggcgtcttcg
     1921 ggcaaccgcc cgcggatccg ctgcagccgg gaacggagcc gttgtacaag ctgaacgcca
     1981 acatcatgca gttctacatc tccgcctact tctaggccgc gccgcgtcgg atctttccga
     2041 cagagcgaaa gcaccactgg ttttcccgcg ttgaccccgg cgcgggactc gcgtatttct
     2101 cggcttctag ctagggggac accaatggat ttgcctcgaa cgcgcgggaa agttgcgcgc
     2161 atcgtggcgc ttggcgtgat tgcgtcggcc gccgtggtgt acgtggccag ggatggcagc
     2221 gagcctccgc gcgaagggat gcgtgcggag gcctcgcttt cgatcgaaca gcccgcagcg
     2281 gtgcaacctt ccacccgggc gacgcgcaca cccgagagcc gtggcgtcga caccccttcc
     2341 gtcccgctgg tcgagctcga gcggcagctt gccgccagcg atcccgccga ccgcgaactc
     2401 gtgctcgaag cggaattgcc cgcgatggtc gcgaagaatc ccgaagagct cgcgcgcttc
     2461 gccgaactgc agaccgaccc caggttgcgc gaactcctga tccgccaggt ggctcaggaa
     2521 tgggcgcaga ccgatctcga tcgcgcgatg gcctgggcca actccctgcc cgactcaccc
     2581 gagcgcgtcg ccacgctcat cgacctgtcg ctgaccgtcg cgcagaccga tccgcaacgc
     2641 gccgtgaata tgcgcgaacc gcaggtcggc aatatcgaac ccgatggcgt cctcgaaaac
     2701 ctggtccagc aatgggccct gcaggatttc gacgcggcgc tcgcgtgggc caacgcacgg
     2761 ccacacaatg cgcagcgcga caaactactg cagcggctcg tgtatgcacg cgcttcggcc
     2821 ggccagcctg cggaagccgc gaagct
//
LOCUS       DATDEU010000619         3179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1145244, whole genome shotgun sequence.
ACCESSION   DATDEU010000619 DATDEU010000000
VERSION     DATDEU010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145244"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VIV63_14820"
     CDS             complement(<1..448)
                     /locus_tag="VIV63_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6125922.1"
                     /translation="MTQSKTQGARLERITASPRWDGTRFRNTSIIPRGDPGVPMPAIS
                     EFLCGGDRRVPQAALPALNPLDAWTRKPDSGLRATWLGHSTVLIEIDGWRVLTDPVWG
                     PRASPSRFLGPKRFQPVPVEVRELPPLDAVIVSHDHYDHLDYTTMRL"
     gene            736..1374
                     /locus_tag="VIV63_14825"
     CDS             736..1374
                     /locus_tag="VIV63_14825"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY6125923.1"
                     /translation="MTEVDLLPRDVFEMSLLETETALRIADSGTDHAKLSLLEDSWNI
                     EASNIPYVRPDRRRFSFCDDCEFQIGVGGTYHSFEGTEGVVIPVTVTWDRSRWELGVF
                     HFSEQTSSNNFENVEQVVARPYWGASLSRRFAFYERGPIRAIFGFGVSYRTEQDVLSA
                     THWNFSSQLGLRFQSPQFPAIIELSARHWSNGGVRTPNRGQDFTILTVRFDR"
     gene            complement(1385..2458)
                     /locus_tag="VIV63_14830"
     CDS             complement(1385..2458)
                     /locus_tag="VIV63_14830"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6125924.1"
                     /translation="MPRFLRLLTVCTLLGLGTARAADYPAPVEADFVIRDFRFSGGES
                     LPELRIHYRTLGTPRKDAKGIVRNAVLIGHGTGGSGAQFIRPEFAGELFGAGQPLDAT
                     RYFIVLADGIGHGKSAKPSDGLRAKFPKYGYTDMVEAQYRLLTEGLKVNHLRLVMGTS
                     MGGMHTWVWGERHPEFMDALMPLASLPVQIAGRNRVWRKTIIDAIRNDPSWQGGNYTT
                     QPPSLGTAASLIYFMGSNPILRQEQMPTLARADEVAGETAANAMKTLDANDVLYQISA
                     SWDYDPAPGLERIRAPLLAINSADDLINPPELGILEREIKRVKAGTAIVIPLSAQTRG
                     HGSHTIAALWKHHLVKLLDDTPQ"
     gene            2698..>3179
                     /locus_tag="VIV63_14835"
     CDS             2698..>3179
                     /locus_tag="VIV63_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125925.1"
                     /translation="MKYGHFLLALGTLALANAASAANRPAGYTTICNENKTCSVAAST
                     NVAFGRADQFLNKTLSGTFVCSSATFGGRIAGGVNECSVPTGSAPPPPPPPPTNPPPG
                     GTADLGYYPGCAMPTNTQVVQLAATQVVPAGTVFDGQNKVYNLTGGSQSEGQPAVFDV
                     "
BASE COUNT          569 a          960 c         1026 g          624 t
ORIGIN      
        1 tcagtcgcat ggtcgtgtag tcgaggtggt cgtagtgatc gtgcgagacg atgaccgcgt
       61 cgagcggcgg caattcgcgc acctcgacgg gaaccggttg aaatcgcttc gggccgagga
      121 aacgcgaagg tgatgcacgc ggtccccaca ccggatcggt gagcacccgc cagccgtcga
      181 tttcgatgag caccgtcgag tgaccgagcc aggtagcgcg caatccgctg tccggcttgc
      241 gcgtccacgc atcgagcggg ttcaacgcgg gcagcgccgc ctggggaacg cgccggtcgc
      301 caccacacag gaattcgctg atcgcgggca tcggcacgcc gggatcgccg cgcggaatga
      361 tgctggtgtt tcggaagcgc gtgccgtccc atcgtgggga ggcggtgatg cgttccagtc
      421 gcgcgccttg agtcttgctt tgggtcattg gggtgctccg ggttgcgact caatgcggat
      481 gaactggcag gtctcaactg gctgacaacg atctcacttg cgcactgaca gtgttctctc
      541 cctaaagagg taggttgctc cccattattt ggagacacga gcgcgacacc ttcggggaat
      601 ttcgattgaa tccgattcag ccgaatatcc gtctcaatga acatgaagaa gaaaccaagg
      661 ggattgaacc gactcggcgt gtttgccgtg cttacgagtc tgttgatggg atccacagcc
      721 gcgatgggcg cgagcttgac cgaggtcgat ctgctgccgc gcgacgtctt cgaaatgtcg
      781 ctgctcgaaa ccgaaacggc acttcgcatc gcggactccg gaaccgacca cgcgaagctc
      841 tcgctgctcg aggattcctg gaacatcgaa gcctccaaca ttccttacgt gcgtccggat
      901 cgccggcgat tttcattctg cgacgactgt gagttccaga tcggcgtcgg cggcacctac
      961 cactcgttcg aaggtaccga aggtgtcgtg attccggtga cggtgacctg ggatcgcagc
     1021 cgctgggaac tcggggtgtt ccatttctcc gagcagacgt cgtcgaacaa tttcgagaat
     1081 gtggagcagg tggtcgcgcg tccctattgg ggcgcctcgc tgtcgcgtcg cttcgcgttc
     1141 tacgaacgcg gcccgatccg cgcgatattc ggcttcggcg tgtcctaccg cacggagcag
     1201 gacgtgctga gcgcgacaca ctggaacttc tcatcgcagc tcgggctgcg tttccagtcg
     1261 cctcagttcc cggcgatcat cgagctctcc gcgcggcact ggtcgaatgg cggcgtacgg
     1321 acgccgaatc gcggccagga cttcaccatc ctgacggtgc gattcgaccg ctgacagtgc
     1381 ccgcttactg cggtgtgtcg tccagtagtt tgacgaggtg atgtttccac agcgccgcga
     1441 tggtgtgcga gccatgaccg cgggtctgcg cactcagcgg aatcacgatg gcagtgccgg
     1501 ctttcacgcg cttgatctcg cgctcgagga tgccgagctc gggtggattg atgaggtcgt
     1561 ctgccgagtt gatcgccagc agtggtgctc gaatccgttc gaggccgggc gcggggtcgt
     1621 agtcccatga cgcgctgatc tggtagagaa cgtcattcgc gtccagcgtc ttcatcgcgt
     1681 tggccgcggt ctcgcctgcc acttcgtcgg ccctggccag cgtcggcatc tgctcctggc
     1741 ggaggatcgg attgctgccc atgaaataga tgagcgaggc ggccgtgccc aacgacggcg
     1801 gctgcgtcgt gtagttgccg ccttgccagg acgggtcgtt ccggatggcg tcgataatgg
     1861 tttttcgcca gacgcgattg cggccggcga tctgcaccgg aaggctggcg agcggcatca
     1921 gcgcgtccat gaattccgga tggcgttcgc cccagaccca ggtgtgcatg ccacccatgg
     1981 aagtgcccat gaccaggcgc aggtgattca ccttcagtcc ttcggtgaga aggcggtatt
     2041 gcgcctcgac catgtcggtg tagccgtatt tcgggaattt cgcgcgcagg ccgtcgctgg
     2101 gcttggcgga tttgccatgg ccgatgccgt cggcgagcac gatgaaatag cgagtggcat
     2161 cgagcggctg gcccgcgcca aacaactcgc ctgcgaattc cggacggatg aactgcgcgc
     2221 cgctgccacc ggtgccgtgg ccgatgagca cggcgttgcg cacgatgccc ttcgcgtctt
     2281 tcctgggagt gccgagcgtc cggtagtgaa tccgaagctc cggcaggctc tcaccacccg
     2341 agaagcgaaa gtcgcggatg acgaaatcgg cttcgaccgg agcagggtag tccgctgccc
     2401 gggcggtgcc caagcctaac aatgtgcaga ccgtcagcag tcgaaggaac cttggcatgg
     2461 acgtactccg tgtgtgaggc cggattatgg gagaagcgcc cgctggtagc gctacagccg
     2521 tggaaaatcc cgcacccgcc tcgctgcgta aatgcactga ttgagatcgg gacgtgtcgc
     2581 ggttcataat gacaccggtg tctacgacta tgacaccggt ggccatggat gcaggccctt
     2641 ccggctctcc tcccgtggct gcctttggat gaaggatcaa ccaaccaggg ggaagcgatg
     2701 aagtacggac attttctatt ggcgttggga acgctggcgt tggcgaatgc ggcatcagcc
     2761 gccaatcgtc ccgccggcta cacgacgatc tgcaacgaaa acaagacttg ctcggtggcc
     2821 gcatcgacca acgtcgcctt cggccgcgcc gatcaatttc tcaacaagac actgagcggc
     2881 accttcgtgt gctcatcggc gacgttcggc ggcaggattg ccggcggcgt caacgagtgt
     2941 tcggtgccca ccggaagcgc gccgccgcca ccgccgcctc cgcccaccaa tccgcctccg
     3001 ggcggtaccg cggatctcgg ttactacccg gggtgcgcca tgccgaccaa cacgcaggtc
     3061 gtgcagctcg ccgcgactca ggtcgttccc gcgggcacgg tgttcgacgg acaaaacaag
     3121 gtctacaact tgacaggcgg tagtcagagt gaaggtcagc ctgcggtgtt cgacgtcat
//
LOCUS       DATDEU010000620         4963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_706287, whole genome shotgun sequence.
ACCESSION   DATDEU010000620 DATDEU010000000
VERSION     DATDEU010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706287"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..280
                     /locus_tag="VIV63_14840"
     CDS             56..280
                     /locus_tag="VIV63_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125926.1"
                     /translation="MSDYYLADLKAEERARQRKREVEQPREPKKSQDMKVTLLGDFLF
                     PTSEPQGCDPYNNTHGKSAREAWRTRRDRR"
     gene            complement(317..1975)
                     /locus_tag="VIV63_14845"
     CDS             complement(317..1975)
                     /locus_tag="VIV63_14845"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY6125927.1"
                     /translation="MSTFERLLRHLVPAILAGVAISAAAAPKSGSIDEAFYRAHIAKL
                     ASDEFGGRKPGTEAEKRTLDYLEEQFRALGLKAGNGASFLQEVPMVEITAASDATLKF
                     TTGENSADLKFREDMVIWTKRVKTAESMESSPMVFVGYGVTAPEYGWDDYAGIDMRGK
                     TAVILVNDPGFITKDDSLFRGRAMTYYGRWTYKYEEAARRGATGAIIVHQPEPAAYGW
                     DTVVNSWSTPQLDNSTADGNAGRVAIEGWITESVARNLFAANGMALDEAVKRANTRGF
                     RAEPMKSTASATLRNAIRRTTSHNLAALLPGTKRPDEYLVYMAHWDHLGRLPGCSGDC
                     VFNGAVDNATGTSGLLTIARAFVSAKKKPERSIVFLAVTLEESGLLGSAHYVSNPLFP
                     LAQTVAAFNMDAMHFGGPTRDVTVVNIGASQLENYLAEAAKAQDRVIRAEPTPEKGTF
                     FRSDHFNFAKGGVPALYIKGGIDDREHGAAWRQKYLDDFTAQKYHKVGDEYSPDADLR
                     AGVEDLALLYAVGAKLAGEKTFPNWNTDSEFRAARDRVRAAAAK"
     gene            complement(1987..2280)
                     /locus_tag="VIV63_14850"
     CDS             complement(1987..2280)
                     /locus_tag="VIV63_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125928.1"
                     /translation="MRPLVVLLGIVMGSTVSIAVAMLLTGVVFLLLPEYGARLAEERN
                     PLLLACLLSVSLAAFAATSFYGELRTRSWRYGAHGALLLMLALALWTYWPKKT"
     gene            2383..4506
                     /locus_tag="VIV63_14855"
     CDS             2383..4506
                     /locus_tag="VIV63_14855"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEY6125929.1"
                     /translation="MNSLITRSTRWLAPSMLAAFMVLTGSAQAPASTQLPAGSVVPSE
                     RHRSVARKVGSMLEGMHYSGSKLDDRLSGIVYSRYLESIDSQHTSLLAADVAEFEQYK
                     LRFDDMIRSGDVDPAFAIFARFQQRNRERIKFAIAQLDKEPNWTLNESFEFNREKAPW
                     AASSAELDDWWRKRVKNDALSLLLTGKDWPKTAEQLKRRYETTLKRIDQISADDVFES
                     LMNSYARAYDPHSSYFSPRSSEEYRIQMSLNYDGIGASLQLQDDYVTIMNVIEGGPAA
                     VAGTLSVKDRITGVGQGKEGPVTDVLGWRIDDVVQLIRGRAGTTVRLQILPGGAAPGS
                     TEKTLEFTRNKVTLEAQAAQKELRTIKRGDKEIRVGVINVPAFYQDVEAQNSGDKEFR
                     STTRDVRRLINELKAEKIDGLVLDLRGNGGGYLPEATALTGLFVDKGPVVQLKVTTGE
                     LEVQDDPEPGVAYSGPLAVLVDRFSASASEIFAGAIQDYHRGVVLGQRTFGKGTVQNL
                     VPLDRWSPRPVNGQLTVTIGKFYRVTGESTQHRGVEPDVTLPSQIDMEEFGESALEGA
                     LPWDRIPGVPFRSEGKSQQPNIAVLATEEKTRADKDPDYRWLVADLAAADVMRKQTSL
                     SLNLVQRKEERERLERERLSRENARRAAQNLPALKNAEELADAKFPDTVLDQAAEVMT
                     DMVTGARPIHSAPTRTVRRDEKKTN"
     gene            4620..>4963
                     /locus_tag="VIV63_14860"
     CDS             4620..>4963
                     /locus_tag="VIV63_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY6125930.1"
                     /translation="MKTALLRAAVIALALAVTACAEEGVAGDKSAAEKDAKALADSAI
                     PVEVTKPVRGEMLAMYSGTATLEAEADAKIVAKVGGEVRRLLVEEGDRVSAGQVLAVL
                     DDRQLRLQAAQTR"
BASE COUNT          968 a         1655 c         1506 g          834 t
ORIGIN      
        1 cgacctccga aattgtgacg caattcggcg acactggccg cggaggatga aaccgatgag
       61 cgattactat ctcgcggacc tgaaggccga ggaacgtgcg cggcaacgca agcgtgaggt
      121 cgaacagccg cgggaaccga aaaagagcca ggacatgaag gtcacgctgt taggcgactt
      181 cctgttcccg acctcggagc cgcaaggctg cgacccgtac aacaacacgc acggcaagtc
      241 cgcgcgcgaa gcctggcgca cgcggcgcga ccgccgctga ctcttcccgg cgcccgaagc
      301 tggcgccgcc ctccgactac ttcgcagccg ccgcgcgaac gcgatctcgc gccgctcgga
      361 attcgctgtc cgtattccag ttcggaaagg tcttttctcc agccagtttc gcgcccacgg
      421 cgtacagcaa tgcgaggtct tcaacgccgg cgcgcaggtc ggcgtcgggt gaatattcgt
      481 cgcccacctt gtgatacttc tgcgccgtga aatcgtcgag gtacttctga cgccaggcgg
      541 caccgtgctc gcgatcgtcg atgccaccct tgatgtacag cgcaggcaca ccgcccttgg
      601 cgaagttgaa atggtccgac cggaagaacg ttcccttttc cggcgtcggt tctgcgcgga
      661 tgacacggtc ctgcgccttc gccgcttccg ccaggtaatt ctcgagctgc gacgcgccga
      721 tgttgaccac ggtgacatcg cgggtcggac ctccgaagtg catcgcgtcc atgttgaatg
      781 ccgcgacggt ttgcgcgagc ggaaacagcg gattgctgac gtaatgcgcc gaacccagca
      841 gacctgattc ctcgagcgtc accgcgagaa acacgatcga tcgctccggc tttttcttcg
      901 cgctgacgaa agccctggcg atggtgagca acccggacgt gcccgtggcg ttgtccacgg
      961 cgccattgaa gacgcaatca cccgaacatc ccggcaaacg ccccaggtga tcccagtgcg
     1021 ccatgtagac gagatattca tcggggcgct tcgtacccgg cagcagcgcc gcgaggttgt
     1081 gagaggtcgt gcggcggatc gcgttgcgca gcgtcgcgct ggccgtgctc ttcatcggct
     1141 cggcgcgaaa tccgcgcgtg ttggcccgct tcaccgcttc gtcgagcgcc atgccatttg
     1201 ccgcgaacag attccgcgcg acagattcgg tgatccaccc ttcgatggcc acccgcccgg
     1261 cattgccatc ggcggtggaa ttgtcgagct gcggcgtcga ccagctgttc acgaccgtgt
     1321 cccagccata ggcagccggc tcgggctggt gaacgatgat ggcgccggtc gcgccgcgtc
     1381 gcgcagcctc ttcatacttg taggtccagc gtccgtagta ggtcattgcc cggccgcgaa
     1441 acagcgaatc gtccttggtg atgaaccccg ggtcgttgac gagaatgacc gcggtctttc
     1501 cacgcatgtc gatgccggcg tagtcgtccc agccatattc gggtgccgtg actccgtagc
     1561 ccacgaacac catcggactc gattccatcg attcagcggt tttcacccgc ttcgtccaga
     1621 tgaccatgtc ttcgcggaat ttgaggtccg ccgagttctc acccgtggtg aacttcaaag
     1681 tcgcgtccga ggccgcggtg atttccacca tgggcacttc ctgcaggaag ctcgcgccgt
     1741 ttccggcctt gagccccaac gcgcggaact gttcctcgag gtaatcgagt gtgcgtttct
     1801 ccgcttccgt gcctggcttg cgccctccaa actcgtcgga tgcaagcttt gcaatgtgcg
     1861 cgcgatagaa cgcctcgtcg atggaacccg atttcggggc ggcggcagcg gagattgcga
     1921 ctccggcaag gattgccgga accaagtgtc ggaggaggcg ctcgaatgta ctcatgtggg
     1981 tgtgtttcac gtcttcttcg gccagtaagt ccagagggca agcgccagca tcaacagcag
     2041 cgctccgtga gcgccatatc gccaggagcg cgtacgcagc tcgccataaa aactcgttgc
     2101 ggcaaaggcc gccagtgaca ccgacagcag gcaggccagc agcagcggat tgcgctcttc
     2161 cgctaggcga gccccgtact ccggtaacag caggaaaacc accccggtca gcagcattgc
     2221 gaccgcgatc gagactgtcg aacccatcac gattccaagg agaacgacca gtgggcgcat
     2281 ggaaatgcct atgtttgatg agatgacact tgagcaggcc gccgcgcggc cttagtgttg
     2341 aattgtacgg atcgtcccga aacttagaga ctcccccgaa gcatgaattc actgatcacc
     2401 cgctcgaccc gctggctcgc tccttcgatg ctggccgcct tcatggtgct cacgggctcc
     2461 gcccaggctc cggccagtac gcagctcccc gcgggctccg tggtgccgag cgagcgccac
     2521 cggtcggttg cacgcaaagt cgggtccatg ctcgaaggca tgcattacag cggttcgaag
     2581 ctcgatgatc gcctctcggg catcgtctat tcacgttacc tcgagagcat cgacagccag
     2641 cacaccagcc tgctcgccgc ggacgtcgcc gagttcgagc aatacaaact ccgcttcgac
     2701 gacatgatcc gcagcggcga cgtcgacccg gcgttcgcca tcttcgcgcg cttccagcag
     2761 cgcaaccgcg agcgcatcaa gttcgccatc gcgcagctcg acaaagagcc gaactggacg
     2821 ctcaacgaat ccttcgagtt caaccgcgaa aaagcgccgt gggccgcctc gagcgccgag
     2881 ctcgacgact ggtggcgcaa gcgcgtcaag aacgacgcgt tgtcgctgct gctgaccggc
     2941 aaggactggc ccaagaccgc cgagcagctc aagcgccgat atgagacgac cttgaagcgc
     3001 atcgaccaga tcagcgccga cgacgtgttc gagtcgctca tgaattccta cgcgcgcgcc
     3061 tacgacccgc actcgagcta cttctcgccg cgcagctccg aggaataccg gatccagatg
     3121 agcctcaact acgatggcat cggcgcgtcg ctgcagctgc aggacgacta cgtgaccatc
     3181 atgaacgtca tcgaaggcgg ccccgcggcc gtcgccggca cgctgtcggt caaggaccgc
     3241 atcaccggtg tcggccaggg caaggaaggt cccgtgaccg acgtgctcgg ctggcgcatc
     3301 gacgacgtcg tgcagctcat ccgcggccgc gcgggcacca ccgtgcgcct gcagatcctc
     3361 ccgggcggcg ccgccccggg cagcaccgag aaaacactcg aattcacgcg caacaaggtg
     3421 accctcgaag cgcaggccgc gcagaaagag ctgcgcacca tcaagcgcgg cgacaaggaa
     3481 atccgcgtcg gcgtcatcaa cgtgccggcg ttctaccagg acgtcgaagc gcagaactcc
     3541 ggtgacaagg aattccgcag caccacgcgc gatgtgcgcc gtctcatcaa cgaactcaag
     3601 gccgagaaga tcgacggtct cgtgctcgac ctccgcggca atggcggtgg ctatctaccc
     3661 gaggcgaccg ccctgaccgg cctgttcgtc gacaagggac cggtcgtgca gctcaaggtc
     3721 accacgggcg aactcgaagt gcaggacgat ccggagcccg gtgtcgccta ttcgggcccg
     3781 ctcgcggtgc tggtcgatcg cttcagtgct tcggcctcgg aaatcttcgc gggcgccatc
     3841 caggactacc atcggggtgt cgttctgggc cagcgcactt tcggcaaggg caccgtgcag
     3901 aacctcgtgc cgctcgaccg ctggtcgccg cgcccggtga acggccagct caccgtcacc
     3961 atcggcaagt tctaccgcgt cactggcgag agcacgcagc atcgcggcgt cgagcccgac
     4021 gtcacgctgc cgtcgcagat cgacatggaa gaattcggcg agagcgcact cgaaggcgcc
     4081 ctgccctggg accgcattcc cggcgtgcct ttccgcagtg aaggcaaatc gcagcagccg
     4141 aacatcgccg tgctcgccac cgaggaaaag acacgcgcgg acaaggaccc tgactaccgc
     4201 tggctggtgg ccgacctcgc cgccgccgac gtcatgcgca aacagacttc gctgtcgctg
     4261 aacctggtgc agcgcaagga agagcgtgag cgcctcgagc gcgaacgtct cagccgcgag
     4321 aacgcccgtc gcgccgcgca gaacctgccg gcgttgaaga acgccgagga actggcggac
     4381 gccaaattcc cggataccgt cctggaccaa gccgcggaag tcatgaccga catggtgacc
     4441 ggcgctcgtc cgatccactc cgcgccaacc cgcaccgtgc gtcgcgacga gaaaaagacc
     4501 aactgacctg ccagaaatgg tgttgtagtt gactacaaca ccaggcttcc ctagacttcc
     4561 gcccagttcg caaccaattg ggtctctgcg tcgtcataac gcagacaaag gggaaggtca
     4621 tgaaaacagc tctcttgcgt gccgccgtca ttgcgctcgc cctggccgtc accgcgtgcg
     4681 cggaagaagg tgtcgccggc gataaatccg cggcggaaaa agacgcaaag gcactggccg
     4741 actcggccat ccccgtcgaa gtgaccaaac ccgtccgcgg cgaaatgctc gcgatgtaca
     4801 gcggcaccgc cacgctcgaa gcggaagcgg acgcgaagat cgtcgccaag gtgggcggcg
     4861 aagtccgccg cctgctcgtc gaggaaggcg accgcgtctc ggcgggccag gtgctcgccg
     4921 ttctcgacga ccgccagttg cgattgcagg ccgctcagac tcg
//
LOCUS       DATDEU010000621         2369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_412722, whole genome shotgun sequence.
ACCESSION   DATDEU010000621 DATDEU010000000
VERSION     DATDEU010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412722"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VIV63_14865"
     CDS             complement(<1..597)
                     /locus_tag="VIV63_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125931.1"
                     /translation="MISSRVLRAVCVVGLCIGLGSNKAHAVGVPAGTDITNVAEVTYT
                     VGATSATASSNAVVVTVAEILDVVVTRQSPLNTAVSASATQQEIVFTVTNSGNGPETF
                     RLVLDSVIGADDFDPTPSTPSIYFDTDASGDLSPGDTAYTAGTNDPVLTPDTSVTILV
                     VNDIPATVVDGNVGITRLTVDSRTGTGTPGTIYAAQGAG"
     gene            853..>2369
                     /locus_tag="VIV63_14870"
     CDS             853..>2369
                     /locus_tag="VIV63_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6125932.1"
                     /translation="MTEIRPAGVEAANENSAQDGFAPADTGAQPELEHAAAADAAEQI
                     LGANPLLGLDRDELLAAGKRLLRLMTINPQVLIEENIAFGRELLAIAMGQSKIAADPR
                     DRRFSHVVWQKNGYYKRLMQGFVAWRGMLNRVLDRADTSTDDRERARFVLQLFTETFA
                     PTNSLFGNPGALQRITETRGKSLLYGVQNFIDDLTNNFGMPRQVDDRKFQVGRNLAVT
                     PGAVVFRGEAFELIQYTPQTDRVHLRPLVIVPPQINKFYATDLAPGRSFAEYAVSQGI
                     QTFCISWRNPTAAQRDWNMETYLSACKQAIAVACDITGADRVNAMAACAGGFTLATLL
                     GHLAAKGDEKVASATLLVTVLDTEAPTLLGQFASRSGVAATIEKSRAKGVLEGSEMAR
                     VFAWLRPNDLVWLFVANNWVMGNRPPAFDILYWNSDTTRLPAEFHADLLRMFMDNPLK
                     SPGKISALGTPIDMSKVTVPAYVVAGVTDHITPWQACYQSRNILRGKIDFVLSSSG"
BASE COUNT          444 a          752 c          757 g          416 t
ORIGIN      
        1 gccggcgccc tgggcggcgt agatcgttcc tggcgtaccg gtgcccgtgc gcgaatcgac
       61 ggtcaggcgc gtgatgccga cgttgccgtc gaccaccgtg gccggaatgt cgttcaccac
      121 gagaatcgtc accgacgtgt cgggggtcag aaccggatcg ttggtgccgg cggtgtacgc
      181 ggtgtcgccg ggcgaaagat cgccgctggc gtccgtgtcg aaatagatag agggggtgga
      241 cggcgtcgga tcgaagtcat cggcgccgat cacgctgtcc aggaccaggc ggaaggtttc
      301 cggaccgttg ccgctgttgg tcacggtgaa gacgatttcc tgctgcgtcg cactcgcgct
      361 caccgcggtg ttgagcggcg actggcgcgt aacgaccacg tcgaggattt ccgcaacggt
      421 gacgaccacg gcattcgagc tcgccgtcgc actggtggcg ccgacggtgt acgtcacttc
      481 cgcaacgttg gtgatgtcag tgcccgccgg aacgccgacc gcatgggcct tgttagaccc
      541 gaggccgatg cacagaccga ctacgcaaac cgcgcgtagc actcgcgatg aaatcaagac
      601 ggaaactcct cgttattcct agtggctggg cgccacttcc ccgcgaccga ttgcgggagg
      661 aggcgtcaaa cccatgacgt tcacgggttt tggggcctcg ctgcgttgca taataggaat
      721 cgaggcccct ggggcacgtg actagggtca cgccgaccct gtcgccagtg ggatacagat
      781 cacatataac gagagcagcc cgagtggaga tcgccgatgg cagccaaaag accccgccaa
      841 gcccgtgaaa acgtgacgga aatccgcccc gcgggggtgg aggcggccaa cgagaattcc
      901 gctcaagatg gcttcgcgcc ggccgataca ggggcgcaac cggaactcga gcacgccgcc
      961 gccgcggatg ccgccgaaca gattctcggc gccaatccct tgttaggcct cgaccgcgac
     1021 gagttgttag ccgccggcaa acgcctgttg cggctcatga cgatcaatcc ccaggtactc
     1081 atcgaagaga acatcgcgtt cggccgcgag ctgctcgcca ttgcaatggg ccagtcgaag
     1141 atcgccgccg atccgcgcga ccggcgattc agtcacgtgg tctggcagaa gaatgggtac
     1201 tacaagcgcc tcatgcaggg cttcgtcgcc tggcgcggga tgctgaaccg cgtgctcgat
     1261 cgtgccgaca cgagcaccga cgatcgcgaa cgggcgcgct tcgtattgca gctgttcacc
     1321 gaaacgtttg cgcccaccaa ctccctgttc ggcaatccgg gagccttgca gcgcatcacc
     1381 gagacgcggg gcaagagcct gctgtacggc gtgcagaatt tcatcgatga cctgaccaac
     1441 aacttcggca tgccccggca ggtggacgac cggaaattcc aggtcggccg gaatctcgcg
     1501 gtgacgccgg gcgcggtggt gtttcgcggc gaggctttcg aactcattca atacacgccg
     1561 caaaccgatc gggtccacct gcggccgctg gtgatcgtgc cgccgcagat caacaagttc
     1621 tacgccacgg atctggcgcc gggccgcagc ttcgccgaat acgccgtgag ccagggcatc
     1681 cagactttct gcatcagctg gcgaaatccc accgccgcgc aacgcgactg gaacatggag
     1741 acctatctgt cggcgtgcaa gcaggcgatc gcggtcgcct gcgacatcac cggcgcggat
     1801 cgcgtgaatg ccatggccgc ttgcgccgga ggcttcacgc ttgccacgtt gctcgggcac
     1861 ctcgccgcga agggcgatga gaaagtggcg agcgccacgt tactggtaac cgtgctcgac
     1921 accgaagcgc cgacgcttct cggtcagttc gcttcccgtt caggcgtcgc cgcgaccatc
     1981 gagaagtccc gtgcgaaagg tgtcctcgaa ggcagcgaga tggcgcgcgt gttcgcctgg
     2041 ttgcggccga atgacctcgt gtggctcttc gtcgccaata actgggtgat gggcaatcgc
     2101 cctcccgcct tcgacatcct ctactggaat tccgacacga cccggctgcc ggcggaattt
     2161 cacgccgacc tgctgcgcat gttcatggac aacccgctca agtcaccggg caagatctcg
     2221 gcactcggca ctccgatcga catgtcgaag gtcacggtgc cggcttacgt ggtggctggt
     2281 gtcacggacc acatcacgcc gtggcaggcg tgttatcaat cgcggaacat cctgcgcggc
     2341 aagatcgatt tcgtgctgtc ctcgagcgg
//
LOCUS       DATDEU010000622         3891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2164600, whole genome shotgun sequence.
ACCESSION   DATDEU010000622 DATDEU010000000
VERSION     DATDEU010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164600"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..3340
                     /locus_tag="VIV63_14875"
     CDS             62..3340
                     /locus_tag="VIV63_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6125933.1"
                     /translation="MAREVGTEGKLGGQANVRDVSGVWKELTENVNVMANNLTEQVRG
                     IVKVVTAVATGDLRQRLTVESKGEVAALGETINSMTGTLAIFADQVTSVAREVGVEGR
                     LGGQAHVAGASGTWKDLVANVNMLAANLTTQVRAIAEVATAVTKGDLTRSIQVEARGE
                     VSELKDNINAMIHNLRHTTDRNTEQDWLKTNLAKFSRMMQGQRDLVTLGETLLAEFAP
                     LVSAHQGVIYIVGYEDGQNYLKQLASYADVRDSAEPRRYRFGEGFVGECAVQRQRLLL
                     ARIPPDSVRLESGLVEAQPRNVIVLPVLYEGEVKAVIELASLYEFTASHLAFLEQLTG
                     SIGVVLNTIEATMRTEGLLKQSQELAGELQTQQVELQQTNEEMATKARLLAEQNAEVE
                     RKNQEIEQARKALEEKAAELALTSRYKTEFLANMSHELRTPLNSILILGQQLSENPEA
                     NLSARQVEFAKTIHAAGTDLLNLISDILDLSKIESGTVTVESEELAFSQLRETIDRNF
                     RHEAESRHLTFTTDFDPRLGRHMVTDAKRLMQIVKNLLSNAMKFTAQGSVRLHVGPAM
                     LGWSADHPVLSQAKNVVEFAVTDTGIGISPEKQKIIFEAFQQADAGTARKYGGTGLGL
                     AISRELAHLLGGEIRLKSSPGSGSTFTLYLPLTYQGAAYGRANTPTFIKQPSSALPAP
                     ALPAPHIERVEDDRDVIEDGDSTVLIVEDEPRYAKMLLEAARGKGFKVLVAQNGADAL
                     SLARKFQPDAITLDVFLPDMLGWTVLNQLKHDPATRHIPVQVLTVEEERQYGLERGAF
                     SFMTKPTSIEGLQSVFTRIQEFIEPRVRELLVIEDDSAERMSVAALIEASDIRISTAE
                     SGARALELLRERKFDCVILDLKLPDISGFELLTEIQRDESLRDVPIVVFTGRELTHEE
                     ETELRQRAKSIVLKGVQSPERLLDETALFLHRKVSDLPEAKQRMIENLHQSDEPLRNR
                     KVLVVDDDVRNIFALNSLLERHGMNVITATNGQDAIKLLDSNPDLSLILTDVMMPEMD
                     GYETMRRIREIPAFRMLPIIALTAKAMKGDREKCLQAGASDYVAKPVNTGQLLSLVRM
                     WLQP"
     gene            3337..>3891
                     /locus_tag="VIV63_14880"
     CDS             3337..>3891
                     /locus_tag="VIV63_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6125934.1"
                     /translation="MNDRAVANILMVDDQPARLLTYRAILEPLGHNLVAVNSGLEALD
                     ALMKQEFAVVLLDVKMPGMDGFETAELIHDHPRFETIPIIFVTGVHVTEFDRLKGYKA
                     GAIDYVYIPVIPEILRSKVAVLVELYAKRRELQGVNKELADSNKALAEANYTLQSEKT
                     RELEELNGHLQRANDELSRSNESLE"
BASE COUNT          802 a         1281 c         1209 g          599 t
ORIGIN      
        1 gaaagatacg ctcaacacga tggttgacca gcttaattca ttcgcgtccg aggtaacacg
       61 cgtggcgcgc gaagtcggca cggaaggaaa actcggcggc caggcgaacg tgcgcgacgt
      121 gtcgggcgtg tggaaggagc tcaccgaaaa cgtcaacgtc atggccaaca acctcacgga
      181 gcaggtgcgc ggcatcgtca aggtggtgac cgcggtcgcc acgggcgact tgcgtcaacg
      241 cctcaccgtc gaatcgaaag gtgaagtcgc cgcgctcggc gaaaccatca actccatgac
      301 gggcacgctc gcgatcttcg ccgaccaggt gacgtccgtg gcacgcgagg tcggcgtcga
      361 aggccgactc ggcggccagg cgcacgtcgc cggcgcgtcc ggcacgtgga aagacctcgt
      421 ggccaacgtg aacatgctgg cggccaatct cacgacccag gtgcgcgcca tcgcggaagt
      481 cgccaccgcc gtgaccaagg gtgacttgac ccgctccatt caggtcgaag cgcgtggcga
      541 agtgtccgag ctcaaggaca acatcaacgc gatgatccac aacctccgtc acacgacgga
      601 ccgcaacacc gagcaggact ggctcaagac caacctcgcc aagttcagcc gcatgatgca
      661 gggccagcgc gatctcgtga cgctgggcga aacgctgctc gcggagttcg cgccgctggt
      721 cagcgcgcac cagggcgtca tctacatcgt cggctacgag gacggtcaga attatctcaa
      781 gcagctcgcg agttacgccg acgtgcgcga ttccgccgag ccgcgccgct atcgctttgg
      841 cgaaggtttc gtcggcgagt gcgcggtgca gcgccagcgc ctgttgctcg cgcgcattcc
      901 accggattcc gtgcgcctcg aaagtggcct cgttgaagcc cagccgcgca acgtcatcgt
      961 gctgcccgtg ttgtacgaag gcgaagtcaa ggcggtcatc gagctcgctt cactttacga
     1021 attcaccgcc tcgcatctcg cgttcctgga gcagctcacc ggatccatcg gcgtggtgct
     1081 caacaccatc gaagccacga tgcgcaccga aggcctgctc aagcagtcgc aggaactcgc
     1141 cggcgaattg cagacgcagc aggtcgagct gcagcagacg aacgaagaga tggccacgaa
     1201 ggcgcggctg ctcgccgagc agaatgccga agtggaacgc aagaaccagg aaatcgagca
     1261 ggcccgcaag gcgctcgaag aaaaggccgc cgagctcgcg ctcacgtcgc ggtacaagac
     1321 cgagttcctc gccaacatgt cgcacgagct gcgcacgccg ctcaacagca tcctcatcct
     1381 gggccagcag ctctccgaga atccggaagc caacctgtcg gcgcgccagg tggaattcgc
     1441 caagactatt cacgcggccg gcaccgacct gctgaatctc atcagcgaca ttctcgatct
     1501 gtcgaagatc gagtcgggca ccgtcacggt cgaaagcgag gagctggcgt tctcgcagct
     1561 gcgcgagacc atcgatcgca acttccgcca cgaggccgag tcgcgtcacc tcacgttcac
     1621 caccgatttc gacccgcgcc tcggccgcca catggtcacc gacgcgaagc gcctcatgca
     1681 gatcgtcaag aacctgttgt cgaacgccat gaaattcacc gcgcagggca gcgtgcgcct
     1741 gcacgtgggt ccggcgatgc tcggctggtc ggccgaccat cccgtgctgt cgcaggccaa
     1801 gaacgtcgtc gaattcgcgg tcaccgacac cggcatcggc atctcgcccg agaagcagaa
     1861 gatcatcttc gaggccttcc agcaggcgga tgcgggcacg gcccgcaaat acggcggtac
     1921 cggcctgggg ctcgccatca gccgcgaact cgcgcacctg ctcggcggcg aaatccgtct
     1981 caagagctcg ccgggatctg gaagcacctt cacgctctat ctgccgctga cctaccaggg
     2041 cgcggcatat ggacgcgcga acacgccgac gttcatcaag cagccgtcgt ccgcactgcc
     2101 ggcgccggcg ctgccggcgc cgcacatcga gcgcgtcgaa gacgaccgcg atgtgatcga
     2161 ggacggcgac agcaccgtgc tgatcgtcga ggacgaaccc cgttacgcaa agatgttgct
     2221 ggaagccgcg cgtggcaaag gcttcaaggt gctggtggcg cagaacggcg cggatgcgct
     2281 gtcgctggcg cgcaagttcc agcccgacgc catcacgctc gacgtgttcc tgccggacat
     2341 gctcggctgg acggtgctga accagctcaa gcacgatccg gcgacgcggc acattcccgt
     2401 gcaggtgctc accgtcgaag aggagcgcca gtacggcctg gaacgcggcg ccttctcgtt
     2461 catgaccaaa cccacgagca tcgaaggcct gcagtcggtg ttcacgcgca tccaggaatt
     2521 catcgagccg cgggtgcgcg aattgctggt catcgaagac gattcggcgg aacgcatgag
     2581 cgtcgcggcg ctcatcgaag cgtccgacat ccggatctcc acggccgaga gcggcgcccg
     2641 cgcactcgaa ctgctgcgcg agcgcaagtt cgactgcgta atcctcgatc tcaagctgcc
     2701 ggacatctcc gggttcgaac tgctgaccga aatccagcgg gatgaaagcc tgcgcgacgt
     2761 gcccatcgtg gtgttcaccg gacgcgagct cacgcacgag gaagagaccg agctgcggca
     2821 gcgcgccaag agcatcgtgc tgaagggcgt gcagagcccg gagcggctgc tcgacgaaac
     2881 cgcgctgttc ctgcatcgca aggtcagcga cctgcccgaa gccaagcagc gcatgatcga
     2941 gaacctgcac cagagcgacg aaccgctgcg caaccgcaag gtgctggtgg tcgacgacga
     3001 cgtgcgcaac atctttgcgc tcaacagcct gctggagcgt cacggcatga acgtgatcac
     3061 cgcgaccaac ggccaggacg cgatcaaact actggacagc aacccggacc tgtcgctgat
     3121 cctgaccgac gtcatgatgc cggagatgga cggctacgaa accatgcggc gcatccgtga
     3181 gattcccgcg ttccggatgt tgcccatcat cgcgctcacc gccaaggcga tgaagggcga
     3241 ccgtgagaaa tgcctgcagg ccggcgcgtc cgactacgtg gcgaagcccg tgaacaccgg
     3301 ccagctcctg tcactcgtgc gcatgtggtt gcagccatga acgatcgggc cgttgcgaat
     3361 atcctgatgg tggacgacca gccggctcgt cttctgacct accgcgcgat cctcgagccc
     3421 ctgggtcaca acctggttgc ggtcaactcc ggcctcgaag cgctcgatgc gctcatgaag
     3481 caggaattcg cggtcgtgtt gctcgacgtg aagatgcccg gcatggacgg cttcgaaacc
     3541 gccgagctca ttcacgacca tccgcgattc gagaccatcc ccatcatctt cgtgaccggc
     3601 gtgcacgtca cggagttcga ccggctcaag ggctacaagg ccggcgccat cgactacgtg
     3661 tacatcccgg tcatcccgga gatcctgcgc agcaaggtcg cggtgctcgt ggagctgtac
     3721 gcgaagcggc gcgagttgca gggcgtcaac aaggagctgg cggactccaa caaggcgctg
     3781 gccgaggcca actacacgct gcagtcggag aagacgcgcg agctcgagga gctcaacggc
     3841 cacctgcagc gtgccaacga cgaactgtcg cgctccaatg agtcgctcga g
//
LOCUS       DATDEU010000623         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_413237, whole genome shotgun sequence.
ACCESSION   DATDEU010000623 DATDEU010000000
VERSION     DATDEU010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413237"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2653
                     /locus_tag="VIV63_14885"
     CDS             <1..>2653
                     /locus_tag="VIV63_14885"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6125935.1"
                     /translation="NTNAPVGRVKGSMDEQNIRLVGRIERPAEFNDIVVKRQGGEIVR
                     LGQVATVEDGFAEPGSYSLRSGQPNVGLSIIRSREASTVTVAERVRKEVEEINKTLPE
                     GTKLEVTRDGGEEASQNLNNVIHALVFGAGLTIFVVYAFLNSWRSTLITGLSLPTSAV
                     AAFIAIWLCGFTLNFMTLLGLSLAIGVLIDDAIVVRENIVRHMERGEDRITAARNGTA
                     EIGLAVTATTFSIVAVFVPVAFMGGGAGEWFRPFAVTVVSSVLVSLFISFTLDPMLSA
                     YWGDPPGYAHSERRGLGKYFARFNTWFDTQANGYGKVIEWALHHRIWMAVGAFALLIL
                     SLGYQGAFGRSEFLPKSDYGTVAIEVRTPSSTSLEYARGKVEKAAELARQIPEVKSTD
                     SRVNATGGRVWVDVGDKTDRERGLGELAADLRTRLKALVGAEYVVLDDLNNGARKPVQ
                     IQFYGTDSRKLMELTNQFMEKMRQIPGAVDVGLSEQDPKDELRIELDRGLANQLGISV
                     NDAAQTLRVAFAGVEVGDWVDPTGESRDVAVRLSPEDRLDASNIEHLPISVTGTNMMI
                     PLEQIATITIDKGPARIQHYNGKRTVTVSANVQGVDAGKVTSEAMKIAKTLEFPAGFG
                     LGLGGASRDQNELFAEMGIALLMGIFVMYLVLVMQFGSFTAPLAIMLSLLFSLIGVVL
                     ALNLTGGSLNLMSLIGVIMLMGLVAKNAILLLDCAREEERQGIDREEALMHAGRVRLR
                     PILMTTFALMAGMMPVAIGVGEGGEFYSPMAVAIIGGTIVSTLLTLLVVPSFYDSIEI
                     ARERAALKFHARSAYRNPLVAFLLTLGEALITLLFIRFFYRSVRWMFNLETPAEHPVE
                     RAARVAGFAMPAGFVARPKPWQRREARH"
BASE COUNT          454 a          854 c          867 g          478 t
ORIGIN      
        1 gaacaccaac gcgccggtgg gccgcgtcaa gggctcgatg gacgagcaga acatccggct
       61 cgtgggccgg atcgagcgcc cggccgagtt caacgacatc gtcgtgaagc gccagggcgg
      121 cgagatcgtg cgccttggcc aggtggcgac cgtcgaagac gggttcgcgg agcctggcag
      181 ctattcgttg cgcagcggcc aacccaacgt gggcctgtcc atcatccgtt cgcgcgaggc
      241 gagcacggtg acggtggcgg agcgcgtgcg caaggaagtc gaagagatca acaagacgct
      301 gcccgagggc acgaagctcg aggtcacgcg cgatggcgga gaagaggcct cgcagaacct
      361 caacaacgtc atccacgcgc tggtctttgg cgccggcctc acgattttcg tcgtctacgc
      421 gttcctgaac tcctggcgtt cgacactcat caccggcctg tccctgccca catccgcggt
      481 cgccgcgttc atcgcgatct ggttgtgcgg cttcacgctg aacttcatga cgttgttagg
      541 tctgtcgctg gccatcggcg tgctcatcga cgacgccatc gtggtgcgcg agaacatagt
      601 tcggcacatg gagcgcggcg aagaccgcat caccgccgcg cgcaacggca cggcggagat
      661 cggcctcgcc gtcacggcca ccacattctc gatcgtcgcg gtgttcgtgc ccgtggcgtt
      721 catgggtggc ggcgccggtg aatggttccg gccgttcgcg gtcaccgtcg tatcgtcggt
      781 gctggtcagt ctcttcatct cgttcacgct cgaccccatg ttgtcggcgt actggggcga
      841 cccgccgggt tatgcgcatt ccgagcgccg cggcctcggc aagtatttcg cccggttcaa
      901 cacctggttc gacacgcagg cgaacggcta cggcaaggtc atcgagtggg cgctgcatca
      961 ccgcatctgg atggcagtgg gcgctttcgc gttgctgatc ctgtcgctcg gataccaggg
     1021 cgcgttcggc cgcagtgaat tcctgccgaa gtccgactac ggaacggttg ccatcgaagt
     1081 gcgcacgccg tcgtccacca gtctcgagta cgcgcgcggc aaggtggaaa aggccgcgga
     1141 actcgcgcgc cagattcccg aagtgaaatc caccgacagc cgcgtcaacg cgaccggcgg
     1201 acgcgtgtgg gtggacgtcg gcgacaagac cgatcgcgag cgcggactcg gcgaactggc
     1261 cgcagatctg cgcacgaggc tcaaggcgct ggtcggcgcg gaatacgtcg tgctcgacga
     1321 cctcaacaac ggcgcgcgca agccggtgca gatccagttc tacgggacgg actcccgcaa
     1381 gctcatggag ctcacgaacc agttcatgga aaagatgcgg cagattcccg gcgccgtcga
     1441 cgtgggcctg tcggagcagg atcccaaaga cgagctgcgt atcgagctcg atcgcggtct
     1501 cgcgaatcag ctcggcattt cggtgaacga cgccgcgcag acgttgcgcg tggcgttcgc
     1561 gggcgtcgaa gtgggcgact gggtggaccc gactggcgaa tcgcgtgacg tcgcggtgcg
     1621 cctgtcaccg gaagaccgcc tcgatgcatc caacatcgaa catctgccga tctccgtgac
     1681 cggcacgaac atgatgatcc cgctcgagca gatcgcgacc atcaccatcg acaagggtcc
     1741 ggcgcgcatc cagcactaca acggcaagcg cactgtgacg gtctccgcca acgtgcaggg
     1801 cgtcgacgcg ggcaaggtga cgagcgaggc gatgaagatc gccaagactc tcgaattccc
     1861 ggccggcttc ggcctggggc tgggtggagc atcgcgcgat cagaacgagc tcttcgccga
     1921 gatgggcatc gcgctgctca tgggcatctt cgtgatgtat ctcgtgctcg tgatgcagtt
     1981 cggttcgttc accgcgccgc tcgccatcat gctgtcgctg ctgttctcgc tgatcggcgt
     2041 cgtgctcgcg ctcaatctca cgggcggctc gctgaacctc atgagtctca tcggcgtgat
     2101 catgctcatg ggactggtgg cgaaaaacgc catcctgctg ctcgactgcg cgcgcgaaga
     2161 agaaaggcag ggcatcgatc gcgaagaagc gctcatgcat gccggccgcg tgcgtctgcg
     2221 ccccatcctc atgaccacgt tcgcgctgat ggccggcatg atgccggtgg ccatcggtgt
     2281 cggtgaaggc ggcgagttct attcgcccat ggcggtggcc atcatcggcg gcaccattgt
     2341 ctccacgctg ctcacgttgc tggtggtccc cagcttctac gacagcatcg agatcgcgcg
     2401 cgagcgcgcg gcgctcaagt tccatgcgcg ttcggcgtac aggaatccgc tcgtggcgtt
     2461 cctcctcacg ctcggcgagg cgctgatcac gctgctgttc atccgcttct tctaccggtc
     2521 tgtgcgctgg atgttcaacc tcgagactcc ggccgaacac cccgtcgagc gcgctgcgcg
     2581 agtcgcgggt ttcgcgatgc ccgcgggttt cgtcgcgcga cccaagccct ggcagcgtcg
     2641 cgaggccagg cac
//
LOCUS       DATDEU010000624         5533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1727583, whole genome shotgun sequence.
ACCESSION   DATDEU010000624 DATDEU010000000
VERSION     DATDEU010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727583"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VIV63_14890"
     CDS             <1..777
                     /locus_tag="VIV63_14890"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY6125936.1"
                     /translation="KVERHEAIHNLTEIIDASDVVMVARGDLGVEMGYAAITGLQKTI
                     IHESRTRNKVVITATQMMESMITSPVPTRAEVSDVANAVMDGTDAVMLSAESAVGKHP
                     VKAIQAMEEVIAGAEKYEITHNRMRHRAEGIVDRTEEAIAMAVMYTSNHMKVRAIVAL
                     TESGTTPLWMSRARSDIPIYAFTRHESTRRRVTLYRGVFPVPFDIPEVRTNELFKAIL
                     TMMLQLELVEKGELVIVTKGELTGITGGTNALTILRVPKD"
     gene            786..3167
                     /locus_tag="VIV63_14895"
     CDS             786..3167
                     /locus_tag="VIV63_14895"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY6125937.1"
                     /translation="MKWLRWLRWTLLGIVVCVALAFGLAWWALRQSLPQLDGTANAPA
                     IGAEAAIERDVRGIPVITASTRTDVAYALGYAHAQDRFFQMDLSRRLAAGELSELFGE
                     VAIRQDRRTRRFGFRAVARRVVEATPEVERAIVDAYSNGVNAGLTSLKSRPWEYLLLR
                     AQPRAWLPEDSVLVVHSMWWQLQYGTLRDELARRRLERASAARSDAIAAHDLIAFVYA
                     GHSDWDTPNYSADSRCVQAACTDSASVLTQSFPAELRFSGAADEGGGEPAAPGSNNWA
                     VAGVHTRSGVALIANDMHLDLGVPAVWYPARLRVTGESALDITGVTLPGTPAVAAGSN
                     GQVAWGFTNSYGDFADARWGKCVSTDYRVRRENISIKGGDTEEVTYHDVGEGVVLDGE
                     EFKDDEASGDCLQAAWLATRPEATNFGLLNLERARSIDDVLELAPHVGIPGQNMVIGD
                     SRGRIAWTLLGRVPETRGPDRLFGPLEFLAAADHPRISDPPVGRLWTANQRVVAGSLE
                     AVLGDDEVDVGAGGYDIGARARQIRDGLLSLTHPATEADMLAIQLDSRALFVARWRDL
                     LLALIDEDAMHGPEHRREFRALVSEGAAQATPDAVGYRLVRTFRTNTLNALWRSLATG
                     LLGEDSKIRRPGQFEAAGWRLVSERPSAVPPPGGGEWRDFLLKRVDATIAELLKECSS
                     LSACEFGKLDPVAVRHPLSGALPLLPRLLDMPTQALAGDHHMPRVQDANFGASERFAV
                     SPGREADGYLELPGGPSGHPLSPFYRSGFDDWVAGKPTPFLPGTAMHRLTLKP"
     gene            3248..3652
                     /locus_tag="VIV63_14900"
     CDS             3248..3652
                     /locus_tag="VIV63_14900"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY6125938.1"
                     /translation="MTHNVSDLGALEREVLQLVWDHGPSTADRVQKALSRPLKESTVR
                     TVLKRLEAKGHLTHSVDNRTFVYEAADTRGRAAARAVKRIVDRFCSGSLEEVLVGLVD
                     TRILDRRELSRLAEKIANAQSGAGDKPKRAKK"
     gene            3649..4860
                     /locus_tag="VIV63_14905"
     CDS             3649..4860
                     /locus_tag="VIV63_14905"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY6125939.1"
                     /translation="MTLEIVLSSALRLLLLSAALWLVLRIARVRNPHAEALVWRLVLM
                     AGLALPALLYWRLAPSFATSLELPVIAAAGAGDPDAPAAAVPVSWLAARVPVTIYLGV
                     SLVLVLRLAWGLAAMWSICRAARPMTASDDVRISARVRSPATFGAIILLPADAHDWPV
                     GTLDAVLAHERAHVRSRDGFWSWLAHLHAAIFWFNPMAWWLQRRLETLAETTSDDAVV
                     AARHDPVAYAALLLDFARQPNLRSVAMSVAESNVPERIDRLLSRIPPAAALSRVARWG
                     AFAAVIPAVVLAASTTKAAPPVEPAAPALTIPDSPPSVGVRLTQPANPDDYYPVVAKH
                     EKVSGYAIVEVDVDALGQLVDARVLNVQPADPRFGFADAALQVARNTRFANASPQTGS
                     LKFMVKFEMAK"
     gene            4922..>5533
                     /locus_tag="VIV63_14910"
     CDS             4922..>5533
                     /locus_tag="VIV63_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002049937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6125940.1"
                     /translation="MTETRFSGTETYVATPDLMMAVNAAVTLGRPLLIKGEPGTGKTQ
                     LAAEIASALKRPLHEWHIKSTSKAHQGLYEYDAVSRLRDSQLGDAKVHDIRNYIVKGS
                     LWEAFDSDVQPVLLIDEIDKADIEFPNDLLRELDRMEFFVHETRELVKARHRPIILIT
                     SNNEKELPDAFLRRCFFHYIRFPDAETMEKIVRVHFPSLKSELL"
BASE COUNT          919 a         1866 c         1815 g          933 t
ORIGIN      
        1 aaggtcgagc gtcacgaggc catccacaat ctcaccgaaa tcatcgacgc gtcggacgtc
       61 gtgatggtcg cgcgcggcga tctcggtgtg gaaatgggct atgcggccat caccggcctg
      121 cagaaaacca tcatccacga atcgcgcacg cgcaataagg tcgtgatcac ggccacgcag
      181 atgatggaat ccatgatcac gagcccggtg cccacgcgcg ccgaagtctc ggacgtcgcc
      241 aacgccgtga tggacggcac cgacgcggtc atgctctcgg cggagagcgc cgtcggcaaa
      301 cacccggtca aggccatcca ggccatggaa gaagtcatcg ccggcgccga gaaatacgag
      361 atcacgcaca accgcatgcg ccatcgcgcg gaaggcatcg tcgatcgcac cgaagaagcc
      421 atcgccatgg cggtgatgta cacctcgaat cacatgaagg tccgcgccat cgttgcgctg
      481 accgaatccg gcaccacgcc gttgtggatg tcgcgcgcgc gttcggacat tcccatctac
      541 gcgttcacgc gtcacgaatc cacgcgccgg cgcgtcacgc tgtatcgcgg cgtgttcccg
      601 gtgcctttcg atatcccgga agtgcgcacc aacgaactgt tcaaggccat cctcacgatg
      661 atgctgcagc tcgagctcgt tgaaaaaggc gaactcgtca tcgtcacgaa gggtgagctc
      721 accggcatca ccggtggcac caacgcgctg accatccttc gcgttccgaa ggactaacca
      781 gtctcatgaa atggctgcgc tggctgcgat ggaccctgct gggcatcgtg gtctgcgtcg
      841 cactggcgtt tggtcttgcc tggtgggcgt tgcgccagag cctgccgcaa ctggacggca
      901 cggcgaacgc gccggcgatc ggcgccgagg cggccatcga gcgcgatgtg cgcggcattc
      961 cggtcatcac cgcctccaca cgcacggatg tcgcctacgc gctggggtac gcgcacgccc
     1021 aggaccgctt tttccagatg gatctgtcgc gccgcctcgc ggccggcgag ttgtccgaac
     1081 tgttcggtga ggtcgccatc aggcaggatc ggcgcacgcg gcgcttcgga tttcgcgcgg
     1141 tggcgcgtcg tgtcgtcgag gccacaccgg aagttgagcg cgccatcgtc gacgcctatt
     1201 cgaatggcgt gaacgccggg ctgacttcgc tgaagtcgcg tccctgggaa tacctgctgc
     1261 tgcgtgcaca gccgcgcgca tggctgccgg aggactcggt gctggtggtg cactcgatgt
     1321 ggtggcagtt gcagtacggc acgttgcgcg acgagctggc acggcggcgc ctcgagcgcg
     1381 catcggccgc gcgcagcgac gccattgccg cgcacgatct catcgcattc gtctatgcgg
     1441 gtcactcgga ctgggacacg cccaactatt cagccgactc gcggtgcgtg caggccgcct
     1501 gcaccgattc cgccagcgtg ctcacgcaat cgtttcccgc agagctgcgc ttcagcggcg
     1561 ccgccgacga gggtggcggc gagccggccg caccgggcag caacaactgg gcggtggccg
     1621 gcgtgcacac gcgatcgggc gtggcgctga tcgccaacga catgcatctc gacttaggcg
     1681 ttcccgcggt gtggtatccg gcgcgcctgc gcgtcacggg cgagtcggca ctggacatca
     1741 ccggcgtgac gctgccgggc acgcccgcgg tggcggcggg atccaacggg caggtggcct
     1801 ggggtttcac caacagctac ggcgatttcg cggatgcgcg ctggggcaag tgcgtgagca
     1861 ccgactatcg cgtgcgccgt gaaaacatct cgatcaaggg tggcgatacc gaagaggtca
     1921 cctatcacga tgtcggcgaa ggtgtggtgc tcgacggcga ggagttcaag gacgatgaag
     1981 cgagcggcga ttgcctgcag gcagcgtggc tggcgacgcg acccgaggcc accaacttcg
     2041 gtctgctgaa cctcgagcgc gcgcgcagca tcgacgacgt cctggagctg gcgccacacg
     2101 tcggaatccc cggccagaac atggtcatcg gcgattcgcg cggccgtatc gcctggacct
     2161 tgttaggccg cgtcccggag acccgcggtc cggaccggct gttcggcccg ctcgaattcc
     2221 tggcggccgc cgatcatccg cgcatttccg atccgccggt cggccggttg tggaccgcga
     2281 atcaacgggt cgtcgccggc tcgctcgaag cggtgctggg cgacgacgaa gtcgatgtgg
     2341 gcgccggtgg ttacgacatc ggcgcgcgtg cgcgccagat ccgcgatgga ttgctgagcc
     2401 tcacgcatcc agccaccgag gccgacatgc tggccatcca gctcgactcc cgcgcgctgt
     2461 tcgtcgcgcg ctggcgcgac ctgctgctgg cgctcatcga cgaagacgcc atgcatgggc
     2521 cggaacatcg ccgcgagttc cgcgcgctgg tcagtgaagg cgcagcccag gcgacgcccg
     2581 atgccgtcgg ctatcgcctg gtgcgcacgt tccgcaccaa cacgctgaac gccctatggc
     2641 gcagtctcgc cacggggttg ttaggcgagg attcgaagat ccgccggccc gggcagttcg
     2701 aggcggccgg ctggcggctg gtcagcgagc gtccttcggc cgtgcccccg ccgggcggcg
     2761 gcgaatggcg cgacttcctg ctgaagcgcg tcgacgccac gatcgccgag ctgttgaagg
     2821 aatgcagcag cctgtctgcc tgcgagttcg gcaagctgga tccggtggcg gtccgccatc
     2881 cgttatcggg cgcgctgccg ctgttgccga ggctgctcga catgccgacc caggctctcg
     2941 ccggcgacca tcacatgccg cgtgttcagg acgccaactt cggtgcttcc gagcgctttg
     3001 cggtgtcgcc gggccgtgag gccgacggtt acctcgaatt gccgggcggg ccctccgggc
     3061 atccgctgtc gccgttctat cgcagcggct tcgacgactg ggtggccggc aaacccacgc
     3121 cattcctgcc gggcaccgcc atgcatcggc tcacgctcaa gccctgattg ctcccgcgag
     3181 aaaaagcgct ggcagtgcgc atccccgtag tgctacgctg tgtagcagta caacgggtag
     3241 caactaaatg acacacaacg tttccgatct gggtgctctc gagcgcgaag tcctgcagct
     3301 cgtgtgggac cacggtccct ccaccgcgga ccgggtgcag aaggcgctct cgcgtccgct
     3361 caaggagtcg acggtacgca cggtgctcaa acggctcgag gccaaagggc acctcacgca
     3421 tagcgtggat aaccgcacct ttgtctacga agccgccgac acgcggggcc gcgctgcggc
     3481 gcgcgcggtg aaacgcatcg tcgaccgctt ctgcagcggc tcgctcgaag aggtgctcgt
     3541 cggcctcgtc gacacgcgca tcctcgaccg gcgtgagctc tcgcgcctcg cggaaaagat
     3601 cgcgaacgcg cagtcaggcg ccggagacaa gccgaagagg gcgaagaaat gacactcgaa
     3661 atcgtgctga gcagtgcgtt gcgcctgttg ctgttgagcg ccgcgctctg gctcgtgttg
     3721 cgaatcgccc gcgtgcgcaa tccgcacgcc gaagcgctgg tctggcggct ggtcctgatg
     3781 gcggggctgg ctttgccagc gctgctctac tggcgtctgg cgccgagttt cgcgacctcg
     3841 ctcgagctgc ccgtgatcgc cgcggccggc gcgggcgacc cggatgcgcc ggctgcggcg
     3901 gtgcccgtct cctggctggc ggcgcgcgtg cccgtgacta tctacctggg cgtctcgcta
     3961 gtgctggtcc tccgactcgc gtggggcctg gcggccatgt ggagcatctg tcgcgcggcc
     4021 cggcccatga cggcttcgga cgacgtgcgc atcagtgcgc gcgttcgcag cccggcgaca
     4081 ttcggcgcca tcatcctgct gcccgccgac gcgcatgact ggcccgtcgg cacactggac
     4141 gccgtcctcg cgcacgagcg cgcgcacgtg cgttcccgcg acggtttctg gtcgtggctg
     4201 gcgcaccttc acgccgcgat cttctggttc aacccgatgg cgtggtggtt gcagcgccgc
     4261 ctcgaaaccc tggcggagac caccagcgac gacgcggtcg tcgccgcacg ccacgatccc
     4321 gtcgcttatg ccgcgttgct gctcgatttc gcccgtcaac ccaacttgag gagcgttgcc
     4381 atgtcagtcg cagaatcgaa tgttcccgag cgcatcgatc gtcttctctc gcgcataccg
     4441 cccgccgccg cgctgtcgcg cgtcgcacgc tggggggctt tcgccgcggt gattccggct
     4501 gtcgtactcg cggcctccac gacgaaggcc gcgccgcccg tggaacccgc ggcgcccgcg
     4561 ctgacgatcc ccgactcgcc gccttccgtg ggcgtgcgac tcacgcaacc ggcaaatcca
     4621 gacgactact acccggtggt cgccaaacac gaaaaggtct cgggttacgc gatcgtcgaa
     4681 gttgacgtcg atgcgctcgg ccaattggtg gatgcgcggg tgttgaatgt gcagcccgcc
     4741 gacccgcgat tcggcttcgc cgacgcggcg ctgcaggtcg cccggaacac ccgtttcgcc
     4801 aatgcgagcc cgcaaacggg ttcgctgaag ttcatggtga aattcgaaat ggcgaagtga
     4861 gagccgcgga tgcccgccgc gtggtggcct tcgccacccg gccgggcata agctcgcggg
     4921 catgaccgaa acgcgcttct ccggcaccga aacctacgtc gccacgcccg atctcatgat
     4981 ggcggtgaac gccgccgtga cgctcggccg cccgctcctc atcaagggcg agcccggcac
     5041 gggcaagaca caactcgcgg cggaaatcgc gagcgccctc aagcggccgt tgcacgagtg
     5101 gcacatcaag tccacgagca aggctcacca gggactctac gaatacgacg ccgtatcgcg
     5161 gctgcgcgat tcgcagctcg gcgacgcgaa agtccacgac atccgcaact acatcgtgaa
     5221 ggggagcctg tgggaggcct tcgattccga cgtgcagccg gtgctgctca tcgacgaaat
     5281 cgacaaggcc gacatcgaat tccccaacga cctgctgcgc gagctcgatc gcatggagtt
     5341 cttcgtgcac gagacgcgcg agctggtgaa agcgcggcac cggccgatca tcctgatcac
     5401 cagcaacaac gaaaaagaat tgcccgatgc cttcctgcgc cgctgcttct ttcactacat
     5461 ccgctttccg gatgcggaga ccatggagaa gatcgtgcgc gtgcattttc cgtcgctcaa
     5521 gtcggagctg ctg
//
LOCUS       DATDEU010000625         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_707049, whole genome shotgun sequence.
ACCESSION   DATDEU010000625 DATDEU010000000
VERSION     DATDEU010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707049"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..253)
                     /locus_tag="VIV63_14915"
     CDS             complement(125..253)
                     /locus_tag="VIV63_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 domain-containing protein"
                     /protein_id="HEY6125941.1"
                     /translation="MKRDDSPESAPLVRRIGWMALIWAVSVAALGVVSLILRWWIR"
     gene            complement(250..1257)
                     /gene="cydB"
                     /locus_tag="VIV63_14920"
     CDS             complement(250..1257)
                     /gene="cydB"
                     /locus_tag="VIV63_14920"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY6125942.1"
                     /translation="MDESTVLTNIWAGILAFAVFMYVVMDGFDLGIGILFPIFGVGRE
                     RDTAMNSIAPVWDGNETWLVLGGGGLMAAFPLAYAIVMSALYAPLIAMLLALVFRGVA
                     FEFRWRDPAHRHIWDFAFTGGSVVAALMQGIMLGALLQGIDVDGRAYGGGWWDWLTPF
                     SLLTGASVVAGYSLLGACWLNWKTDGETQAHARRLMRILAPVTLAAIVAVSVATLFLG
                     GEYLERWFAMPAVLVTAQVPLIITILIWRFARSLRRNYELAPFVLTLVVFGLSFVGLG
                     ISMYPYLIPNSITLQMAAAPESSQAFMLVGAGVLVPLILAYTAWSYWVFRGKVAHEGY
                     H"
     gene            complement(1250..>2301)
                     /locus_tag="VIV63_14925"
     CDS             complement(1250..>2301)
                     /locus_tag="VIV63_14925"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY6125943.1"
                     /translation="KRLHFFATLMVAIGTFISAFWILSVNSWMQTPTGYAMNADGQFV
                     PAAGWAAIIFNPSFPYRLVHTVIAAYLTTALAVGDVGAWHLLKNSRHEHARKMFSMAM
                     WMAALVAPVQIFVGDLHGLNTLEHQPAKVMAMEGHYQSHPAGAPLILFGIPNDDAQRM
                     DHAIEIPKLSSLILKHDLDAPLNGLDTVAPENRPPVEVVFWTFRVMVGLGFLMLLLGA
                     CSLLARASRKLYDWRWLLRFALLMGPSGFVAVIAGWMTTEVGRQPFTIYGLLRTADSV
                     SPLAAPAVAVSLIAFVIVYFAVFGMGTWYLLKLMSHAPQPHEPDLPNAPAHAAGITPG
                     PAMAAGASPKEFPHG"
BASE COUNT          481 a          744 c          736 g          340 t
ORIGIN      
        1 tccgggaaat ggggacatcc ctcatttcct tgtaacaatt ctcacaagcc aggcggtcca
       61 agtcgcgccg ccaattgtta caaggaaatg agggatgtcc ccatttccgg agccagaccc
      121 cggcttatct tatccaccag cgcaggatca agctaactac tccgagcgcg gcgacgctca
      181 cggcccagat gagggccatc cagccgatac gccgcacgag tggcgcactt tcgggactgt
      241 cgtcgcgctt caatggtagc cctcgtgcgc caccttgccg cggaacaccc agtacgacca
      301 cgccgtgtac gcgaggatca gcggcacgag cacgcccgcg cccaccaaca tgaatgcctg
      361 gctgctttcg ggcgctgccg ccatctgcag cgtgatgctg ttgggaatga gatagggata
      421 catgctgatg ccgagcccga cgaacgagag gccgaacacc acgagcgtga gcacgaacgg
      481 cgcgagctca tagttcctgc gcagactgcg cgcgaagcgc cagatgagga tggtgatgat
      541 gagcggcacc tgcgccgtca cgaggaccgc gggcatcgcg aaccagcgct cgaggtattc
      601 gccaccgagg aacaacgtgg ccacgctcac tgcgacgatg gccgcgagcg tgaccggcgc
      661 caggatgcgc atgagccggc gcgcatgcgc ctgggtctcg ccgtcggtct tccagttgag
      721 ccagcacgca cctaacaagg agtagcccgc gaccacgctc gcgccggtca gcaggctgaa
      781 cggcgtgagc cagtcccacc agccgccgcc atacgcgcgg ccatcgacgt cgataccctg
      841 cagcagcgcg ccgagcatga ttccctgcat cagcgcggcc actaccgacc cgcccgtgaa
      901 tgcgaagtcc cagatgtgcc gatgcgcggg atcgcgccag cgaaactcga aggcgacgcc
      961 gcgaaacacg agcgccagca gcatggcgat cagcggcgcg tacagcgccg acatcacgat
     1021 tgcataggcg agcggaaatg cggccatcaa accgccgccc ccgagcacca gccacgtttc
     1081 gttgccgtcc cacaccggcg cgatggaatt catggcggtg tcgcgctcgc ggccgacgcc
     1141 gaatatcgga aagaggatgc cgatgccgag atcgaagcca tccatgacca cgtacatgaa
     1201 cacggcgaac gcaaggatgc cggcccagat gttggtgagt accgtgctct catccatgag
     1261 ggaactcctt cgggcttgcg ccggcggcca tggccggccc cggcgtgatt cccgcggcat
     1321 gcgccggcgc attgggcaaa tccggctcgt gcggttgcgg cgcgtgcgac atgagtttga
     1381 gcaggtacca ggtgcccatg ccgaacaccg cgaagtagac gatgacgaac gcgatgagcg
     1441 agacggcgac cgcgggcgct gccagcggcg aaacggaatc cgcggtgcgc agcaggccgt
     1501 agatggtgaa gggttgccgg ccgacctccg tggtcatcca gccggcaatc acggcgacga
     1561 aacccgacgg ccccatcaac agcgcgaatc gcagcagcca gcgccagtcg tagagcttgc
     1621 gggaagcgcg cgcgagcagg ctgcacgcac ctagcaacaa catgaggaac cccagaccca
     1681 ccatgacgcg gaacgtccag aacaccacct ccacgggcgg acgattctcc ggcgccaccg
     1741 tgtcgaggcc gttcagtggc gcgtcgagat catgcttgag gatcagcgaa gaaagtttgg
     1801 ggatctcgat ggcgtgatcc atgcgctgcg cgtcgtcgtt cgggatgccg aaaagaatga
     1861 gcggcgcacc ggccggatgg ctctggtagt gcccttccat ggccatgacc ttcgcgggct
     1921 gatgctcgag cgtgttgaga ccgtgaagat cgccgacgaa gatctgaacc ggcgctacca
     1981 gcgcagccat ccacatcgcc atggagaaca tcttgcgcgc atgctcgtga cgcgaattct
     2041 tcaacaggtg ccaggcgccc acgtcaccca cggcgagcgc ggtagtgaga taggcggcga
     2101 tgaccgtgtg caccaggcgg tagggaaagc tcgggttgaa gatgatggcg gcccagcccg
     2161 cggccggcac gaactggccg tccgcgttca tggcgtatcc cgtcggcgtc tgcatccagc
     2221 tgttgacgct caagatccag aacgccgaga tgaacgtgcc gatggccacc atgagcgtgg
     2281 cgaagaagtg cagacgtttg c
//
LOCUS       DATDEU010000626         4443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_413344, whole genome shotgun sequence.
ACCESSION   DATDEU010000626 DATDEU010000000
VERSION     DATDEU010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413344"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VIV63_14930"
     CDS             complement(<1..529)
                     /locus_tag="VIV63_14930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY6125944.1"
                     /translation="MRVTIFGSGYVGLVTGACLADAGNDVLCVDVDAAKIEGLKRGVI
                     PIHEPGLDALIKSNADAERLRFTTSAADGVAHGQLQLIAVGTPPDEDGSADLKYVLAV
                     ARTIGEHMTEYKVVVTKSTVPVGTADKVGASVSESLQKRNAEIEFDVVSNPEFLKEGA
                     AIEDFMRPDRVVVGAD"
     gene            complement(628..2541)
                     /locus_tag="VIV63_14935"
     CDS             complement(628..2541)
                     /locus_tag="VIV63_14935"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEY6125945.1"
                     /translation="MVMVLADCVALPFALFSALALRFGTIDHPIESAQWLYLASVLIT
                     VPIFIRIGLYRAVVRFMGVNAALTIAAGVSISTLLLTLVDYAAFDRSLPIGVLIIYFA
                     LALLYVGASRFVARELLRLRGSSVERVAIYGAGAAGAQLCSSLMSSVQFRPVALLDDN
                     TSVHGARVCGLVVLAPDSLIDLRRRKGVNFVLLALPSASRRRRGEIIERLTAQGFKVQ
                     TIPDLAEIVSGKSRVEEVRDIDVHDLLGRDPVPPNKGLLSACVRGKSILVTGAGGSIG
                     SELCRQILELAPRCLVLLEVSELALYEIERELRSNNVRLGNNIELVPLLGNAHHKHRV
                     REIMTTFGIQTVYHAAAYKHVPIVESNMIEGIHNNIFATYNTAEAALECRVETFVLVS
                     TDKAVNPTNVMGCTKRVAEMVLQGLHQRNSGTRFCMVRFGNVLESSGSVVPLFREQIR
                     DGGPITVTHPDVIRYFMTIPEAAQLVIQAGSMGSGGDVFVLDMGSPVRIADLAKRMVL
                     LAGYTVRDDKNAEGDIEIRFTGLRPAEKLFEELLIGKSVTGTEHPRILRAMEQSLTWE
                     QVRHVLDELAMCSTRFDCERAREVLVRAVPEYQPIDKVQDHVWLRGGSRISGAVLADD
                     EKVREISAFRPRP"
     gene            complement(2877..3437)
                     /locus_tag="VIV63_14940"
     CDS             complement(2877..3437)
                     /locus_tag="VIV63_14940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019112620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY6125946.1"
                     /translation="MKRAFDLLLALGAAVVLVPLALLVALAVAVTSRGPILYWSDRVG
                     RNNVNFRMPKFRSMRVGTPPVATHLLADADRHLTPIGSFIRRSSLDELPQLWSILRGH
                     MSFVGPRPALYNQEDLIALRTARGVHVLTPGLTGWAQINGRDELPIPVKVEFDAVYLA
                     RRSFWFDLQILWRTAINVLRRTGVSH"
     gene            complement(3421..4377)
                     /locus_tag="VIV63_14945"
     CDS             complement(3421..4377)
                     /locus_tag="VIV63_14945"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6125947.1"
                     /translation="MIFITGANGFVGSALGGRLVRDGRRVIGAVRSPQAALPPGVETF
                     VSGDLQSAGGSLAIPAGVSCVVHTAARVHMLDDTSSDPLAEFRRMNVDATLALARAAR
                     DAGVSRFVFVSSVKVNGEATLPGRPFRIDDPAAPLDPYGVSKCEAEVALRELARGSTL
                     EVAVVRPPLVYGPGVRANFHALMRLLRSGLPLPFGAIDNRRSLIGIDNLVDVLTTCID
                     APAAANLTFLVSDDEDVSTTELMRRLGRALGVKPRLIPVPPRLLTAGAALVGKSERMR
                     RLCESLQVDVSATKQALGWRPRVALDQGLASTAEHYLRETRV"
BASE COUNT          850 a         1386 c         1444 g          763 t
ORIGIN      
        1 tgtccgcacc cacgaccacg cggtccggcc gcatgaagtc ctcgatggcc gcgccttcct
       61 tgaggaattc ggggttcgag accacgtcga attctatttc ggcgttgcgc ttctgcagcg
      121 actcgctcac ggacgcgccc accttgtccg cggtaccgac cgggacggtc gacttggtga
      181 ccaccacctt gtattcggtc atgtgctcgc cgatggtccg cgcgactgcc agcacgtact
      241 tgagatcggc tgaaccgtcc tcgtccggcg gcgttcccac cgcgatgagt tgcagctggc
      301 cgtgcgccac gccgtcggcc gccgaggtcg tgaaccgcag gcgttccgca tcggcgttcg
      361 acttgatcag cgcatcgagc ccgggctcgt ggatgggaat gacgccgcgc ttgaggcctt
      421 cgatcttggc ggcatcgacg tctacgcaca gcacgtcatt gccggcatcg gcaaggcatg
      481 caccggtgac caatccgaca tagccggatc cgaaaatggt gactcgcatg tattccctga
      541 ttcagcgcca aacaagcgcg aggtccggga ttctaaccaa tcctcattac aagagtgccg
      601 tgagcactcg taggagtcga tgagtggtta cggcctgggc cggaacgcgg agatttcgcg
      661 gactttttcg tcgtcggcga gcactgcacc actgatgcgc gagcctccgc gcagccagac
      721 gtgatcctgc accttgtcga ttggctgata ttcgggcacg gcacgcacca aaacctcgcg
      781 tgcgcgctcg caatcgaacc gtgttgaaca catcgccagt tcgtcgagca cgtgccgcac
      841 ctgctcccag gtcaggctct gctccatggc gcgaaggatg cgcgggtgtt cggtgcccgt
      901 gacgctcttg ccgatgagca gctcctcgaa gagcttctcg gccggacgca ggccggtgaa
      961 gcggatttcg atgtcgccct cggcgttctt gtcgtcgcgc acggtgtaac cggccagcag
     1021 caccatgcgc ttcgcgagat cggcgatgcg taccgggctg cccatgtcga ggacgaacac
     1081 gtcaccaccc gaacccatcg aacccgcctg gatgacgagc tgcgcggctt cggggatggt
     1141 catgaaatag cggatgacgt ccggatgcgt gacggtgatc ggaccgccat cgcgaatctg
     1201 ttcgcggaac agcggcacca ccgaacccga cgactcgagc acgttgccga agcgcaccat
     1261 gcagaaccgc gttccgctgt tgcgctggtg caggccctgc agaaccattt cggcgacgcg
     1321 cttcgtgcag cccatcacgt tggtgggatt cacggccttg tccgtcgaaa cgagcacaaa
     1381 ggtttccacg cggcactcga gcgccgcctc cgccgtgttg tacgtggcga agatgttgtt
     1441 gtggatgccc tcgatcatgt tgctttccac gatgggcacg tgtttatacg cggcggcgtg
     1501 ataaacggtc tggatgccga acgtggtcat gatctcgcgc acgcgatgtt tgtggtgcgc
     1561 gttgcctaac agaggcacga gttcgatgtt gttgccgagc cggacgttgt tcgaacgcag
     1621 ttcgcgctcg atttcgtaga gcgcgagctc cgacacttcg agcagcacca ggcagcgcgg
     1681 cgcgagctcg aggatctggc ggcacagctc ggagccgatg gagccaccgg cgccggtcac
     1741 caggatggat ttgccgcgca cgcaggcgga cagcagaccc ttgttcggcg gtaccggatc
     1801 gcggcccagc aggtcgtgca cgtcgatgtc gcgcacttcc tcgactcgcg attttccgga
     1861 gacgatttcc gccagatcgg ggatggtctg aaccttgaaa ccctgcgcgg tcagtctttc
     1921 gatgatttcg ccgcgacggc ggcgcgacgc gctgggcaac gccagcagca cgaagttgac
     1981 gcctttgcgc cggcggagat cgatcagtga gtcgggtgcg agcaccacga gaccacagac
     2041 acgcgcaccg tggacgctgg tgttgtcgtc gagcagtgcg accggacgga actgcacgct
     2101 gctcatgagc gatgaacaga gctgagcgcc tgcggcgccc gcgccgtaga tagcgacgcg
     2161 ctcgaccgac gaaccgcgaa ggcgcaacaa ctcgcgcgca acgaaccgcg atgcgccgac
     2221 gtagagcaac gcgagtgcga agtagatgat gagcacgccg atgggcagcg acctgtcgaa
     2281 agcggcgtaa tcgaccagcg tgagcagcaa cgtggagatg ctgacgcccg cggcgatggt
     2341 cagcgcggcg ttgacgccca tgaaacgcac gaccgcgcgg tacagaccga tgcggatgaa
     2401 gatggggacc gtgatcagga ctgacgcgag gtacagccac tgcgcggatt cgatggggtg
     2461 atcgatggtg ccgaagcgca acgcgagcgc ggagaacagc gcgaacggca gggccacgca
     2521 atcggcgagg accatgacca tctgtttgga gcggcgatga agtctgcgtg cgttcccgac
     2581 gatcctgact gtggttcttc gtactcgccg ggcgcgccgc atgttgcttt tgtatcgcaa
     2641 gttcaagtcc gcatcacatc catgtcgtaa atcggcgtga aatctaccaa atcagcggtc
     2701 ggaaaggtgg ttatcgaggt cggacagcaa cgcgagtcgt tcaaatgagg caagtcccta
     2761 gggtgacgat gacgtctctg tcgcgagacg ctgttttatc tcaccaattt gatgaaaaag
     2821 ctgtggaaat gtgtcttccg cgatcgagaa cgagtacaga agttacaaag ttgtcgctaa
     2881 tgagatacgc ccgttcggcg caaaacgttg atagcggtcc gccagagaat ctgcaggtcg
     2941 aaccagaacg agcgacgggc gagataaacg gcatcgaact cgaccttgac gggtatgggt
     3001 agttcgtcgc gaccgttgat ctgagcccat ccggtgaggc cgggagtcag gacatggacg
     3061 ccgcgggccg tgcgcagcgc aatcaggtct tcctggttgt acagcgccgg gcgtggcccg
     3121 acgaaactca tgtggccgcg cagaatgctc cagagctgcg gcaactcatc gaggctgctc
     3181 cggcggatga acgaaccaat gggcgtcaga tgacgatcgg cgtccgccag taggtgggtg
     3241 gccacgggcg gagtgcccac gcgcatgctg cgaaattttg gcatccggaa gttcacgttg
     3301 ttccggccaa cgcgatccga ccagtagagg attggtccgc gcgaagtcac ggcgacggca
     3361 agcgccacca gcaatgccag cggcaccagg acgacggcgg caccgagcgc cagcagaaga
     3421 tcaaacgcgc gtttcacgca gatagtgctc cgcagtactg gccagaccct gatcgagcgc
     3481 gactcgcggc cgccacccga gcgcctgctt tgtcgccgac acgtccacct ggaggctttc
     3541 acacaggcgg cgcatgcgtt cggacttgcc gaccagcgcg gcccccgccg tcagaagccg
     3601 cggtggcacg gggatgagac gcggcttcac gcccagcgcc ctgcccagcc gccgcatcag
     3661 ttcggtggtc gatacgtctt cgtcatccga gacgaggaaa gtcaggttgg ccgcggccgg
     3721 tgcgtcgatg caggtagtga ggacatcgac gaggttgtcg atgccgatga ggctgcggcg
     3781 attgtcgatc gcgccgaacg gcaatggcaa tccggaacgc agcaaccgca tgagtgcgtg
     3841 gaaattcgcc cgtacgcccg gtccatagac cagcggcggc ctgactaccg cgacttcgag
     3901 tgtcgaaccg cgggccaact cccggagcgc gacctcggcc tcgcacttgg agacgccata
     3961 agggtcgagg ggcgcggcgg gatcgtcgat gcggaacggc cggccgggca acgtggcttc
     4021 gccgttcacc ttgaccgaac tcacgaacac gaaacgcgaa acacccgcgt cccgggccgc
     4081 gcgcgccagc gccagcgtgg cgtcgacatt catccggcga aattccgcga gcgggtcgct
     4141 gctcgtgtca tcgagcatgt gcacgcgcgc cgcggtgtgc accacgcagg agacgcccgc
     4201 cggtatcgcc aacgaaccac ctgccgactg caggtcgccc gacacgaatg tttcgacgcc
     4261 gggcggcagg gctgcctgcg gactgcgcac cgcgccgatc acccggcgcc cgtcgcgcac
     4321 caggcggccc cccagcgccg agccgacgaa accattggcg ccggtgatga atatcatggc
     4381 gtgccgttcc cgcgctcgcg cgcggtttcg atgaggtcgc gcgccatgtc ccgcgcgatg
     4441 gcc
//
LOCUS       DATDEU010000627         8648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_998751, whole genome shotgun sequence.
ACCESSION   DATDEU010000627 DATDEU010000000
VERSION     DATDEU010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998751"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VIV63_14950"
     CDS             complement(<1..227)
                     /locus_tag="VIV63_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125948.1"
                     /translation="MRRRAALPWLAALFVVPALGAPAMDPFANPVKDEAALRAALSRP
                     AAKLLNAQVLRGQFQHSRHLKEIPRPLIATG"
     gene            complement(224..637)
                     /locus_tag="VIV63_14955"
     CDS             complement(224..637)
                     /locus_tag="VIV63_14955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY6125949.1"
                     /translation="MTVHAATVEIVVPFHDVDSAGMVWHGHYAKYFEIARCALLDSFN
                     YGYAAMRESGFMWPVIDMRLRYVQAIRFEQRILVRATLREWENRLLIDYLISDHASGQ
                     RLTKGSTVQVAVDMKSGEMLLVSPSILFERLGVAR"
     gene            complement(634..1389)
                     /locus_tag="VIV63_14960"
     CDS             complement(634..1389)
                     /locus_tag="VIV63_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6125950.1"
                     /translation="MKTCIIIPFYNHAGAIAHVVDALKPLGLPCFIVDDGSDVEARHV
                     LAQIAQNERWVTVQTLPENRGKGAAVMAGCDAALAAGFTHALQIDADGQHDVRDAARL
                     LDEARLDPAAMISGEPVYDASAPRSRRYGRYLTHVWIWINTLSFEIRDSMCGLRVYPL
                     AAVCAIWKESRIGQRMDFDPEIMVRLCWRGVRIVAVPTKVHYPRDGVSHFRMFEDNVW
                     ISRMHTRLFFGMLWRAPSLLSRRLFRRSSRVAA"
     gene            complement(1386..1727)
                     /locus_tag="VIV63_14965"
     CDS             complement(1386..1727)
                     /locus_tag="VIV63_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125951.1"
                     /translation="MLLLPSIDAVEREQDHLELRFVVPESNCYFEGHFSGCPVLPGVV
                     QVGWAIEFARQHIPFAARFRALSAVKFMRVIQPNAAVTLRIKADAGLHALSFEYRLAG
                     EPCSSGRVLFH"
     gene            complement(1735..2004)
                     /locus_tag="VIV63_14970"
     CDS             complement(1735..2004)
                     /locus_tag="VIV63_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY6125952.1"
                     /translation="MSVTSKSREEILDHLRTVLSDMFELKPEQIVPEASLYEDLDLDS
                     IDAVDLFLTLKEITGRKIQPQAFKDVRTVSDVIDAIQKLTSPPAG"
     gene            complement(1995..2855)
                     /locus_tag="VIV63_14975"
     CDS             complement(1995..2855)
                     /locus_tag="VIV63_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6125953.1"
                     /translation="MSGTGRKLNHVWRIFATGFVFVLFGAGALVISITMFPLLRLSTL
                     NADTARRRIQRGMQVTFSVYMEIMRVLGILTYRVDGAERLREPGRLIVANHPTLLDVV
                     LLVSQMPAVDCIVKRGLWRNPFLRWPVSWAGYLPNSEGEALIEECSATLRRGHSLLVF
                     PEGTRTVPGQPLRMQRGAAHIALAANSEILPVTIACDPPTLFKGNPWYRVPARRFHLH
                     VVVGAPIAARDFLRGDEAPARAARRLTAWMLTNFDAGRTLRQDPDEVPVALVDVRGST
                     VDQIEMLKCQ"
     gene            3022..3303
                     /locus_tag="VIV63_14980"
     CDS             3022..3303
                     /locus_tag="VIV63_14980"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY6125954.1"
                     /translation="MSQQTAEELLMAQRLVQVLGLEHIAPDAINPSAPLFGSSVDSIG
                     LDSIDALEIALMIQQHYGVELRSEDAAARAAFGSLRSLTEHALRSRAAG"
     gene            3337..3930
                     /locus_tag="VIV63_14985"
     CDS             3337..3930
                     /locus_tag="VIV63_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125955.1"
                     /translation="MFWLALGYPLLAHLAVLTDDRRLEWLALVWLLGIAVSGALIQRR
                     PWAWALMLAGGGLLYWLVVEGNGLYALYIPPAAIPAALFMLFASSLRAGEIPLVTRIA
                     RLMHDGPLPDDLVLYTRHVTQLWCCICAAMFASAVLLAVFASPRLWSLMTNVVHYLVL
                     GAVFVLEYGYRRWRYSHHEHSGLFQYLRRLTRTKLKV"
     gene            3935..5539
                     /locus_tag="VIV63_14990"
     CDS             3935..5539
                     /locus_tag="VIV63_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6125956.1"
                     /translation="MQTLRLLAIDNLDAPIAQYRDAVVTAAQFVAQAEHVARAFPTAS
                     HVVNLSENRYHFLLGFVAACLREQVTLLPPGSTSDVLAKLRREYPDHHTLDDESIARL
                     VAQAADSRTETIPSHWSVPASRVVAIAFTSGSTGASTPHPKTWRSLFHNSRLAAAEVL
                     GGTNRSLVSTVPPQHMYGFEASLLSALTAQSFIHDGKPFFPADVRRALESMPAPRVLV
                     TTPAHLKILADASAELPALHRVVSATAPLPVELARRIEESWQTELVEIYGCTEAGVMA
                     HRRPSQSESWRTFEGGSMTTSGAAEYVAPQLDGPVPLQDVLELRSATEFLLRGRATDM
                     IKVAGKRASLQDLTRQILDVPGVKDAVVFLPDDDARPAAAVVAPGVSAEAILHELRAH
                     LDGVFVPRPLLVVDSLPRNAVGKLPREVLMQLIGRQPATTRVHDITIPATHASLAGHF
                     PGQPIVPAVVLLEAVLAAVQSRDTVVLQSIAATKFLQPVLPDERIELRIQFSAIDPGR
                     RRASFQGLREAALVFEGSFIVAEPAS"
     gene            5536..6462
                     /locus_tag="VIV63_14995"
     CDS             5536..6462
                     /locus_tag="VIV63_14995"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125957.1"
                     /translation="MKDWRSHKERSTPSMVRIIVRLATTLRRPVVRPLLYPIVAYFLL
                     TSPAARKASRDYLRRVLNRPPGWRELWRHFFTFASCTLDRIFILSKRHQSIDVEVDRP
                     EEVRAAVARQTGCLLFVAHFGSAESLRLIAVDRRGLPLSILLDRQHGRMLTELLEELN
                     PELANSIIDASARGPQLVLTLKEAVQAGRMLGIMVDRALATDRSVTVDFLGGRARVPV
                     GPWQMAHALQVPIILGFGVYHGGNRYTAHFELFAESLHLPRADREAAIVSAAQRYAQR
                     LEHHVRQSPYNWFNFYDYWQPAPPADHESAAR"
     gene            6443..7621
                     /locus_tag="VIV63_15000"
     CDS             6443..7621
                     /locus_tag="VIV63_15000"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY6125958.1"
                     /translation="MNPLRVSTYTVSSAVGAGRAAHAQALRTGTTGLGRVAFDTNSLD
                     CWLGEVKGFDVPLTGALAEWDCRNNRLAEFALNQDHFIDAVAACRSRYGAARIGVFVG
                     TSTSGVQHAELAYRARDPQTLALPAWFDQRRTQNVFSVAAFTALRLGLSGPAFAISTA
                     CSSSAKVFAAASRAIASGACDAAVVGGVDSLCLTTLYGFNSLQLISADVCRPADAARM
                     GISIGEAGGFALLDPDAGNPLALLGYGETSDAFHMSSPEPAGRGAIESMRAALSRAGI
                     RPDEVDYINLHGTGTVANDNAESRAVCEVFGAAMPCSSTKGWTGHTLGAAGMVEAAIS
                     LIAIEHGFMPRSLNTRTRDPAIEAGILLETRMAPVRRVLSNSFGFGGSNCSLLFGSVQ
                     "
     gene            7618..8409
                     /locus_tag="VIV63_15005"
     CDS             7618..8409
                     /locus_tag="VIV63_15005"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY6125959.1"
                     /translation="MNAVFIESVGLAAPGLPSWREGLATLRGEQPYVDTPLPPHTPLL
                     LPLNERRRATTAIRLAFQAAEDATQATALPAASLATVFASSDADINIIHRICLALSAT
                     PRLISPTDFHNSVHNAAAGYWSIAAGARAASTTLSAHDGSFGAGLMEACSQVLVDGCN
                     VLLVVFDIPAPEPLNATRFIACPAAAALVLTRQPTAQSLAALACATTVDEEARIEDPA
                     LEQLRLGNPATRALPLLRALAMRQFGQVVLPMTPGANLAIDLRAP"
     gene            8406..>8648
                     /locus_tag="VIV63_15010"
     CDS             8406..>8648
                     /locus_tag="VIV63_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125960.1"
                     /translation="MSELEGLIPHAGSMCLLERVVAHSAQDIECTATSHTSPENPLRR
                     DGQLSAVHLVEYAAQAMAAHGALAAGGVQRGMLASLR"
BASE COUNT         1417 a         2950 c         2774 g         1507 t
ORIGIN      
        1 tcgccggtgg cgatcagcgg acgcgggatt tccttcaggt gtcggctgtg ctggaactgt
       61 ccgcgaagca cctgggcatt gagcagtttt gcggcgggtc gcgacaacgc cgcgcgcagc
      121 gccgcttcgt ccttcaccgg attcgcgaac ggatccatgg ccggcgcgcc aagcgcgggg
      181 acgacgaaca gcgccgcgag ccacggcagc gcggcgcggc gcctcatcgc gccactccga
      241 gacgttcgaa caggatggat ggcgacacga gcagcatctc accgctcttc atgtcgaccg
      301 cgacctgcac ggtgctgccc ttggtcagtc gttgtccgct ggcgtggtcg ctgatcaggt
      361 agtcgatgag caggcggttc tcccattcgc gcaaggtggc cctgaccagg atgcgctgct
      421 cgaagcggat cgcctgcacg taacggagac gcatgtcgat caccggccac atgaaaccgg
      481 attcgcgcat ggcggcataa ccgtagttga aactgtcgag caacgcgcag cgcgcgatct
      541 cgaagtactt ggcgtaatgc ccgtgccaga ccatgcccgc cgagtcgacg tcgtgaaacg
      601 gaacgacgat ctccacggtg gccgcgtgca ccgtcatgcg gccacccgcg agcttcggcg
      661 gaacaggcga cgcgacagca gcgacggcgc ccgccacaac attccgaaga agagacgcgt
      721 gtgcatgcgg ctgatccaga cgttgtcttc gaacatccgg aagtgcgaca cgccatcgcg
      781 cggatagtgg accttggtag gcaccgcgac gatgcgcacg cccctccagc agagccgcac
      841 catgatctcg gggtcgaaat ccatgcgctg gccgatgcgg gattctttcc agatcgcgca
      901 gaccgccgcc agtggataga cgcgaaggcc gcacatcgag tcgcggattt cgaacgacag
      961 cgtattgatc cagatccaca cgtgcgtgag gtagcggcca taacgccgcg agcgcggcgc
     1021 gcttgcgtcg tagaccggct cgccactgat catcgccgcc gggtcgagac gcgcttcgtc
     1081 gagcaggcgc gccgcgtcgc gcacgtcgtg ttgtccgtcg gcgtctatct gcaacgcgtg
     1141 cgtgaaaccc gcggccagcg ccgcgtcgca acccgccatg acggccgcgc ctttgccgcg
     1201 gttctccggc agggtctgga ccgtcaccca gcgctcgttc tgcgcgatct gcgcgaggac
     1261 atgcctcgcc tcgacatccg agccgtcatc gacgatgaaa cagggcagac ccagtggttt
     1321 gagcgcatcc accacgtgcg caatggcgcc ggcatggttg tagaacggaa tgatgatgca
     1381 ggtcttcaat ggaacagcac gcggccgctc gaacagggct cgcccgccag ccggtattcg
     1441 aatgaaagtg cgtggagtcc ggcgtcggcc ttgatacgca gcgtgacggc ggcattcggc
     1501 tggatgacac gcatgaactt caccgccgac agcgcgcgga agcgcgcggc gaaggggatg
     1561 tgctggcgcg cgaactcgat cgcccagcca acctgcacga cacccggcaa caccgggcag
     1621 cctgaaaaat ggccttcgaa ataacagttg gactcgggga cgacgaagcg cagttcgagg
     1681 tgatcctgct cgcgctccac cgcgtcgatc gaaggaagca ggagcacggc ggtctcaacc
     1741 cgccggtggc gacgtcagct tctggatcgc gtcgatgacg tcgctgaccg tgcgcacgtc
     1801 cttgaatgcc tgcggctgga tcttgcggcc ggtgatttcc ttgagcgtga ggaacaggtc
     1861 gaccgcgtcg atgctgtcga gatcgaggtc ctcgtacagg ctggcctccg gcacgatctg
     1921 ctcgggcttg agttcgaaca tgtcgctcag caccgttcgc aggtgatcaa ggatttcttc
     1981 gcgtgatttg ctcgtcactg acatttcaac atctctatct gatccacggt tgaacctcgc
     2041 acgtcgacga gagccaccgg gacttcatcg ggatcctgac gcagcgtgcg cccggcatcg
     2101 aagttcgtga gcatccacgc cgtgaggcgg cgcgcggcgc gcgccggcgc ttcgtcgccg
     2161 cgcaggaaat cgcgcgcggc gatcggcgcg ccgaccacca cgtgcagatg aaatcgccgg
     2221 gccggcacgc ggtaccacgg gttgcccttg aacagcgtcg gcggatcgca ggcgatcgtc
     2281 acgggcagga tctcggagtt ggcggccagc gcgatgtgcg ccgcgccgcg ctgcattcgc
     2341 aatggctgac ccggcaccgt gcgcgtgcct tcggggaaca ccagcagcga atggccgcgc
     2401 cgcagagtcg ccgagcactc ctcgatgagc gcctcgccct cggagttggg tagatagccg
     2461 gcccaggaca cgggccagcg caggaacgga ttgcgccaca ggcctcgctt gacgatgcag
     2521 tccacggccg gcatctgcga caccagcaac acgacgtcga gcagcgtggg atggttggcg
     2581 acgatcagcc gtcccggttc gcgcagtcgc tcggcgccgt cgacgcgata ggtgaggatg
     2641 ccgagcacgc gcatgatttc catgtacacg ctgaacgtga cctgcatgcc gcgctggatc
     2701 cggcggcgcg cggtgtccgc gttcaacgtc gacagccgca gcagcgggaa catggtgatg
     2761 ctgatgacga gcgcaccggc gccgaagagc acgaacacga atccggtggc gaatatccgc
     2821 cacacgtgat tgagtttgcg gcctgttccc gacattgatc tcacgccctc ccgcccgcga
     2881 ttccgtcact aatctcgttg tattccgcgt cgatgcaatc gcgcgatgtg gtggcttgag
     2941 tatagactgt cgcgccgcgc gcgtgacccc ggcgcctccg tacgcgtggc gcaacagata
     3001 actccaacac agaaaatgac catgtcccag cagaccgcgg aagaattgct catggcgcaa
     3061 cgcctggttc aggttctcgg cctcgaacat attgctccgg acgcgatcaa cccttccgct
     3121 cccctcttcg gcagcagcgt cgacagcatc ggactcgatt ccatcgacgc cctggagatt
     3181 gccctcatga tccagcagca ctatggcgtg gaactccgct ccgaagacgc cgcggcccgg
     3241 gccgcgttcg ggtccttgcg ctcgctgacc gagcacgcgc ttcgcagccg cgctgcgggc
     3301 tgagctcccg ccggggcgaa cggacgcacg cgcggcatgt tctggctcgc cctcggctat
     3361 cccctgctgg cgcacctcgc ggtgctcacc gacgatcggc gcctcgaatg gctggccctc
     3421 gtctggttgc tcggcatcgc cgtgagcggc gcgctgatcc agcgccggcc gtgggcgtgg
     3481 gctctcatgc tggctggcgg cgggctgctg tactggctgg tcgtcgaggg caacggtctg
     3541 tacgcgctct acattccgcc ggccgccatc ccggccgcgt tgttcatgct gttcgcgtcg
     3601 tcgctcaggg ccggtgaaat tccgctggtc acgcgcatcg cgcgcctcat gcacgatggg
     3661 ccgttgcccg acgatctggt gctctacacc cgccacgtca cccagctctg gtgctgcatc
     3721 tgcgccgcga tgttcgcctc ggcagtgctg cttgcggtgt tcgcctcgcc gcgcctgtgg
     3781 tcgctgatga cgaacgtggt ccattatctg gtcctcggtg cggttttcgt tctcgagtac
     3841 ggatatcgcc gctggcgcta cagccaccac gaacattccg ggctgttcca atacctgcgg
     3901 cgcctgacgc gcacgaaact caaggtctga aggaatgcag acgcttcgat tgctggccat
     3961 cgacaacctc gatgccccga tcgcgcaata ccgcgatgcg gtcgtgaccg cggcgcaatt
     4021 cgtcgcgcag gccgaacacg tggcgcgcgc gttccccacc gcttcgcacg tggtcaatct
     4081 gtccgaaaat cgataccact tcctgctggg ctttgtcgca gcctgcctgc gtgaacaggt
     4141 gacgctcctg ccgccgggca gtacctccga cgtgctcgcg aaattgcggc gtgaatatcc
     4201 ggaccatcac acgctcgatg acgaatcgat cgcgcgcctg gtcgcgcagg cggcggacag
     4261 ccgaaccgag acgattccgt cgcactggtc cgtgccggcc tcgcgcgtcg tggcgatcgc
     4321 gttcacgtcc ggctcgaccg gcgcctcgac gccccatccg aaaacctggc gcagcctgtt
     4381 tcacaattcg cggctggcgg ccgccgaagt actgggcggc acgaatcgct cgctcgtctc
     4441 cacggtgccg ccgcaacaca tgtatggctt cgaggcatcg ttgttgagcg cactcaccgc
     4501 gcagagtttt attcacgacg gtaaaccttt ttttcccgcc gacgtgcgcc gcgcgctcga
     4561 atcaatgccc gcgccgcgcg tgctggtcac cacgcctgca catctgaaaa tcctcgccga
     4621 tgcctcggcc gaactaccgg cgcttcaccg cgtggtctcg gccacggcgc cattgccggt
     4681 ggagctggcg cggcgcatcg aggagtcctg gcaaaccgaa ctcgtggaga tctacggttg
     4741 caccgaagcc ggcgtcatgg cgcatcgccg gccttcgcag tcggaaagct ggcgtacgtt
     4801 cgagggcggc agcatgacga cgtcgggcgc cgccgagtac gtcgcgccgc agctcgatgg
     4861 ccccgtgccg ctgcaggatg tgctcgagct gcgcagcgcc accgagttcc tgcttcgcgg
     4921 ccgcgccacc gacatgatca aggtggccgg caagcgcgcg tcgctgcagg acctcacgcg
     4981 ccagatcctc gacgtgcccg gcgtgaaaga tgccgtcgtg ttccttcccg atgacgacgc
     5041 gcggcccgcc gccgccgtgg tggcgcccgg cgtttccgcg gaggcgatcc tgcacgagct
     5101 gcgtgcgcat ctcgatggcg tattcgttcc gcggccgctg ctggtcgtcg acagtctgcc
     5161 ccgcaacgcc gttggcaaac tcccgcgtga agtgctgatg caactgatcg gcaggcaacc
     5221 cgcgacgacg cgcgtccacg acatcaccat ccccgcgact catgcttcgc tcgccggaca
     5281 ttttccgggc cagcccatcg tgccggccgt ggtgttgctc gaagccgtgc tcgcggccgt
     5341 acaatcgcgc gacaccgtcg tgctgcagtc gattgcggcg accaagttcc tgcaaccggt
     5401 gctgccggat gaacgcatcg aattgcgtat ccagttcagc gccatcgacc ccggacgccg
     5461 gcgcgcgagc tttcagggat tgcgcgaagc ggcgctggtc ttcgaaggat cattcatcgt
     5521 cgcggagccg gcatcgtgaa ggactggcgc agccacaagg agcgaagcac accgtcgatg
     5581 gtgcggatca tcgtgcggct cgccacgacg ctgcggcgtc cggtagttcg gccgctgctg
     5641 tacccgatcg ttgcgtactt cctgctcacg agcccagccg ccaggaaggc gtcgcgtgac
     5701 tacctgcgtc gtgtattgaa tcgccccccg ggctggcgag aactgtggcg ccatttcttc
     5761 acgttcgcga gttgcacgct cgatcgcatc ttcatcctgt cgaagcggca ccaatccatc
     5821 gacgtcgagg tcgaccggcc ggaggaagtg cgcgccgccg tcgcacgtca gaccggctgc
     5881 ctgttgttcg tcgcccactt cggcagtgcc gagtcgttgc gcctgatcgc ggtggaccgg
     5941 cgcgggctgc cgctgtcgat cctgctcgat cgtcaacacg gtcgcatgct caccgaacta
     6001 ctcgaggagc tgaacccgga gctcgccaac agcatcatcg acgcatcggc gcgcgggccg
     6061 cagctcgtgc tcacgctgaa agaagcggtg caggcggggc gcatgctcgg gatcatggtc
     6121 gatcgcgcgc tcgcgaccga ccgttcggtg accgtcgact tcctcggcgg ccgcgcgcgc
     6181 gttccggttg gcccgtggca gatggcgcac gcgctgcagg tcccgatcat cctgggtttc
     6241 ggcgtttatc acggtggcaa tcgctacacc gcgcacttcg aactattcgc cgaatcgctg
     6301 cacctgccgc gtgcggatcg cgaagccgcc atcgtgagcg ccgcgcagcg ttatgcgcag
     6361 cgtctcgaac atcacgtgcg ccagtcgcct tacaactggt tcaatttcta cgactactgg
     6421 cagcccgcgc cgcccgccga tcatgaatcc gctgcgcgtt agtacctaca ccgtttcgtc
     6481 cgcggtgggc gccggccgtg ccgcgcatgc gcaggcgctg cgcacgggca ccacggggct
     6541 cggccgcgtg gcgttcgaca ccaacagtct cgactgctgg ctcggcgaag tgaaaggctt
     6601 cgacgtcccg ctcacgggcg cgctcgcgga atgggactgc cgcaacaacc gcctcgcgga
     6661 gttcgcgctc aaccaggacc acttcatcga cgccgtcgcg gcctgccgct cgcgttacgg
     6721 cgccgcgcgg atcggcgtgt tcgtgggcac cagcacttcg ggcgtgcaac atgcggagct
     6781 cgcctatcgc gcgcgcgatc cgcagaccct ggcgttgccc gcgtggttcg accagcggcg
     6841 aacgcagaac gtcttttcgg tggcggcgtt caccgcgctg cggctcgggc tctcgggacc
     6901 cgcgttcgcg atatccaccg cctgctcgtc gagcgcaaag gtcttcgccg ccgcgagccg
     6961 cgcgatcgcg tcgggtgcgt gtgatgccgc cgtggtcggc ggcgtcgaca gcctctgcct
     7021 cactactctc tatggcttca actccctgca gctcatctcc gcggacgtct gccgtcccgc
     7081 ggatgccgcg cgcatgggga tttcgattgg cgaagccggc ggattcgcgt tgctcgatcc
     7141 cgacgccggg aatccgctcg cgctgctcgg ctatggagaa accagcgatg cgtttcacat
     7201 gtcttcgccc gagcccgcgg gccgtggcgc catcgagtcc atgcgcgccg cgctgtcgcg
     7261 cgcgggcatc cggcctgatg aagtcgacta catcaacttg cacggcacgg gcacggtcgc
     7321 gaatgacaac gccgagagcc gcgcggtctg cgaggtattc ggcgccgcga tgccctgcag
     7381 ttcgacgaaa ggctggaccg gacacacgct cggcgccgcc ggcatggtgg aagccgccat
     7441 cagcctgatc gcgatcgaac acgggttcat gccgcgaagc ctgaatacgc gcacgagaga
     7501 tccggccatc gaggccggga tcctgctcga gacccgcatg gcgcccgtgc ggcgcgtact
     7561 cagcaattct ttcggcttcg gcggcagcaa ctgcagcctg ctgttcggaa gcgtgcagtg
     7621 aacgcggtgt tcatcgaatc cgtgggtctc gcggcaccgg gattgccgtc gtggcgcgaa
     7681 ggcctcgcaa cgttgcgcgg cgaacaaccc tacgtcgaca cgccgctgcc accgcataca
     7741 ccactgcttc tgccgctcaa cgaacgccgg cgcgcgacca cagccatacg actggcgttt
     7801 caggctgctg aagacgccac gcaggccacg gcactgcccg ccgcgagtct cgcgaccgtg
     7861 ttcgcgtctt cggacgcgga catcaacatc atccatcgca tctgcctcgc gttgagcgcg
     7921 acgccacgcc tcatctcgcc gacggatttc cacaactcgg tgcacaacgc ggcggcggga
     7981 tactggagca ttgccgcggg cgcgcgcgcg gcatcgacca cgctgagcgc gcacgacggc
     8041 tctttcggtg ccggattgat ggaggcctgc tcccaggtgc tggtagatgg gtgcaacgtg
     8101 ctgctcgtcg tgttcgacat acccgcgccc gaaccgctga acgccacacg tttcatcgcc
     8161 tgtccggccg cggccgcact ggtgctgacg cgccaaccta ctgcacagtc gttggccgcg
     8221 ctggcctgcg ctacgaccgt cgatgaagaa gcgagaatcg aggacccggc gctcgagcag
     8281 ttgcgactcg gcaatccggc gactcgcgca cttccgctgc tgcgagcgct ggcgatgcgg
     8341 cagttcggcc aggtggtctt gccgatgact ccgggcgcca acctggccat cgacctgcgc
     8401 gcgccatgag cgagctcgaa ggtctcatcc ctcacgccgg cagcatgtgc ctgctggagc
     8461 gcgtcgtggc gcattcggcg caggacatcg aatgcacggc gacctcgcat acgtcgcccg
     8521 aaaatccact tcgccgcgac ggccaactct cggcagtgca tctggtcgaa tacgcggcgc
     8581 aggccatggc ggcgcatggc gccctggcag ccggtggcgt tcagcgcggc atgctggcgt
     8641 cgctgcgc
//
LOCUS       DATDEU010000628         9402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1582237, whole genome shotgun sequence.
ACCESSION   DATDEU010000628 DATDEU010000000
VERSION     DATDEU010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582237"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VIV63_15015"
     CDS             complement(<1..623)
                     /locus_tag="VIV63_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY6125961.1"
                     /translation="MNDLTKNILLWVVIVMVMVVVFSRYVPTGAEPTQVAYSTVLDDV
                     RNGRVEAVTLRGEEILGVRKDKTKFKAFNPETSNNVLIGMLDKANVDFKGEPPKQPNF
                     LMQLLLQLAPALLLILVFVYMLRQMQGSAGGRGAMSFGKSRARLLGEDQVNVTFADVA
                     GVDEAKQEVGEIVDFLKDPSKFQKLGGKIPKGVLMVGSPGTGKTLLAR"
     gene            807..1181
                     /locus_tag="VIV63_15020"
     CDS             807..1181
                     /locus_tag="VIV63_15020"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEY6125962.1"
                     /translation="MGFKSLEARSLLFDLRGTSNLYNAAMELSERQRKYLRGLGHELN
                     PVLLIGNAGVTAAVIAEAKRALHDHELIKVKFRGAEREARDAGLAELAANTESVLLQR
                     IGHTALYYKRRNDRPGIVIPDA"
     gene            1295..3943
                     /locus_tag="VIV63_15025"
     CDS             1295..3943
                     /locus_tag="VIV63_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6125963.1"
                     /translation="MAAQHTRSFGREAAAVYLAAALVVFGTWAASRYLILRKFENIEQ
                     NEVSRSIEVMRKALVTKNTEIETVSRDFARWDDMYSYVSSLDPNFEYQNFSEAGLDEM
                     NVDMVWLLDPAEKLLSSFENDTDDAQYRHPASDAVVAEINRLTPIVRTVLDQEGTLRL
                     VLINGVPHVIAAHTVLRSDRSGPAGGTLVFARRIGAPEVASVGADTQLDVKFALLDDR
                     AMAEDKAELLVAPDGRRILRRSDNFIQGQLRLDSIDNRPLAVLYTTLPRTVMQNGKQG
                     VSYLVGWVALLISIVVAAWHAYSGRLRRTSAAAATSETRYRAIFDRAASGIVLFDPHS
                     RRILDANPQAQRLIDASLDELRRRDVATIFDTPVSLSAGDHEQPAENRPAQLIRGDDE
                     RRDVEFSVVDLDAGTGRVHAMLLQDISHRREAERRAQEHQLSLQHLATHDALTGLPNR
                     TFLNEELSRLIDEAARRGDSLSILYIDCDNFKNINDSRGHSIGDDYLRAVARRLRDSI
                     ASPDLVVRMGGDEFLVVTRHYGLEPDSAAIAERVAAALKKPVRLGEATYSATTSIGLS
                     VYPRDATTMSELLRSADIALYEAKARGRDNVQMFDSAMNKRVHTRLALEQDLRSAVNR
                     EAIEIQLQPIVDIRSGRLVSFEALARWNDPRHGSVPPLQFIEVAEASDLIVELGECVF
                     RNVARQIAAWQRAGLKPVPVAVNVSAKQLKRSNLPERLVAIAHEFDIAPHQMEVELTE
                     SVAMQDSEQHVTKLHALRDLGVRVSVDDFGTGYSSLAYLRNLPIDYLKIDRTFVRDMN
                     VDRNDAAIVRAIISMAQSLHLGTVAEGIETREHASQLLALGCSTGQGYFFAKPMAAHE
                     TNELLRTMRGAAPVTTGPSLEIVRVS"
     gene            complement(3952..5007)
                     /locus_tag="VIV63_15030"
     CDS             complement(3952..5007)
                     /locus_tag="VIV63_15030"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY6125964.1"
                     /translation="MKRFAVLVVGVVALGASPLVLAQDWIALQDGKSLAGWKPAERPE
                     SWVVEDGAFVSRGDRSHLFYVGKVGKHDFRNFEFQAEVMTSPGANSGIYVHTKWQGPG
                     WPEAGYELQVINSNPPASTMNGYIEYKMTASVYAVRNTWQAPVADNVWFNYRIRVVGK
                     TIQTFINDQLICEYAEPADAFRPKDKKGRLLSSGTFALQAHDPASVVKYRNMKVRILP
                     NDATPPAGLVPLADSALDQLITQASNDNIPLIDLGMSAPANGADEFWSQARRYGVLPL
                     AIQQIPNIGGSVAVLVDRDRGPDVELLKSLKARGYRVAFSSGGVTSFDEAQLKRRLQA
                     ITDAGLGWKDFWVPGKP"
     gene            complement(5041..5517)
                     /gene="greA"
                     /locus_tag="VIV63_15035"
     CDS             complement(5041..5517)
                     /gene="greA"
                     /locus_tag="VIV63_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY6125965.1"
                     /translation="MKRTPMTLRGAEALRNELKRLKGEDRPRVIKAIAEARSHGDLSE
                     NAEYHAAREQQGFIEGRIGEIEAKLSTAEVIDTAGLTNTGKVIFGATVDLEDQDDGKR
                     ISYQIVGEDEADIKTGRISITSPIARAIVGKTVGDVVDVVAPGGKHSYEVMEVRYV"
     gene            complement(5514..8741)
                     /gene="carB"
                     /locus_tag="VIV63_15040"
     CDS             complement(5514..8741)
                     /gene="carB"
                     /locus_tag="VIV63_15040"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY6125966.1"
                     /translation="MPKRTDIKSILIIGAGPIIIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADSIYIEPINWRTLARIIEKEEPDALLPTMGGQTALNTALDL
                     VREGVLARHGVELIAASREAIDMAEDRELFRNAMREIGLECPRAGVAHTLPQAMEIQQ
                     GLGFPCVIRPSFTMGGSGGGIAYNREEFEQIVERGLDASPTSEVLIEESVIGWKEFEM
                     EVVRDKNDNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRNASIAVLRKIG
                     VDTGGSNVQFAVNPKDGRLLVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LKNDITGGATPASFEPSIDYVVTKIPRFTFEKFPAANSRLTTQMKSVGEVMAIGRTFQ
                     ESLQKALRGLETGLDGLNEQFKEMPLAEDDRDKLAYELRAPGPNRMLYVADAFRAGWS
                     RERIEELSYIDPWFLAQIEDLIIEEASVREGGVAALETDRLRDLKRKGFSDSRLGTLT
                     GAGEAAIRAKRHALNIRPVFKRVDTCAAEFATSTAYLYSTYEEECEAQPTKKRKIMIL
                     GGGPNRIGQGIEFDYCCVHAALQLREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTL
                     EDVLEVIAKEQPEGVIVQYGGQTPLKLSRALEAAGAPIIGTSPDSIDMAEDRERFQVL
                     VKNLGLKQPANATARTEDQAVALARTVGFPLVVRPSYVLGGRAMEVVFNEEDLRNYMT
                     HAVKVSNDSPVLLDRFLDVAIEVDVDACCDGEDVLIGGIMEHVEQAGVHSGDSGCSLP
                     PNSLSPEIQAELREQTRKLAKALNVIGLMNIQFAIQAGIIYVLEVNPRASRTAPFVSK
                     ATGLQLAKIGARVMTGKKMKNMENIAEVVPPYFSVKEAVFPFNKFPESDPILGPEMKS
                     TGEVMGTGRTFGEAYAKAQAASGVTLPRSGVVLISVRERDKAGAVVLAKKLIEHGFEL
                     AATEGTAKAITDAGFACKRANKVREGRPHIVDMIKNDEFAMIVNTTEGKTAILESNSI
                     RREAVHKRVTYYTTLAGALATCEALDHLDAVEVNRLQDLHKEARS"
     gene            complement(8760..>9402)
                     /locus_tag="VIV63_15045"
     CDS             complement(8760..>9402)
                     /locus_tag="VIV63_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEY6125967.1"
                     /translation="NRPVMRAHQRLHVVAFDYGVKRNILRMLSDRGCRMTVVPAQTTA
                     EEVLALEPDGVFLSNGPGDPEPCDYAIEAIRALVVAELPTFGICLGHQLLGLASGART
                     MKMKFGHHGANHPVQDLETGRVFITSQNHGFAVDESDLPSTLKATHRSLFDGSLQGIA
                     RIDKPAFSFQGHPEASPGPQEMGAMFDRFVHLMLRRLQAKLRGPRRPPPNEAA"
BASE COUNT         1649 a         2990 c         2944 g         1819 t
ORIGIN      
        1 cgcgccagca gcgtcttgcc ggtacccggc gagcccacca tgagcacgcc cttcgggatc
       61 ttgccgccta acttctgaaa ttttgacggg tccttgagga agtcgacgat ctcgcccact
      121 tcctgtttgg cttcgtcgac gcccgcgaca tccgcgaacg tgacgttgac ctggtcctcg
      181 ccgagcaatc tcgcgcggct cttgccgaaa gacatggcgc cgcggccacc ggccgagcct
      241 tgcatctggc gcagcatgta gacgaacacg agtatgagca gcagcgcggg cgcgagctgc
      301 aacagcaact gcatgaggaa gttcggttgc ttcggcggct cgcccttgaa gtcgacgttg
      361 gccttgtcga gcatgccgat gagcacgttg ttcgaggtct cgggattgaa ggccttgaac
      421 ttcgtcttgt ccttgcgtac tccgaggatc tcctcgccgc gcaaagtgac tgcttccacg
      481 cgtccatttc gcacgtcgtc gagcacggtc gagtacgcaa cctgcgtcgg ttcggcgccg
      541 gtgggaacgt agcggctgaa caccaccacc atcaccatca cgatcacgac ccagagcagg
      601 atatttttcg tcagatcatt caaaggctta ccctcgtaag gcgaccgggc catttaagga
      661 gtgaggtcac gctacaccaa tcgaaaaccg ctcgccagca ggtaaagctc agaactacgt
      721 gaccgggaca tccggtttgg cgatgcgata cgtggagaaa ctatcgaagc ttgcagccct
      781 ggagcgtttt gatgaggacc aatgggttgg gtttcaagag cctggaggcg agatcactgc
      841 ttttcgacct gcgaggcacc agcaatcttt acaatgcagc catggaactt tcagaacgcc
      901 agcgcaagta tttacggggt ttgggtcacg agttgaaccc agtactgctc atcgggaacg
      961 cgggagtgac tgccgcggtc atcgccgagg ccaaacgcgc cctgcacgac cacgaactca
     1021 tcaaggtgaa gttccggggt gcggagcgtg aagcccggga cgcgggcctc gccgaactcg
     1081 cggcaaatac cgaaagcgtg ctgctccaac gcatcggtca tacagctctc tactacaaac
     1141 gccgaaatga ccgtccggga attgtgatcc ccgacgcata aagggctgac gccgctggcc
     1201 gattccctat cactggggtg aggatcacct cgcgggagtt attgccgaac ttccagtggt
     1261 aagttgctgg aataagtcgt atatcagtca aaacatggcg gctcaacaca cacgctcgtt
     1321 tgggcgcgaa gctgcggcgg tctatctagc tgccgcgctg gtcgtattcg gcacctgggc
     1381 agcatcgcgc tacctcatcc tgcgcaagtt cgagaacatc gagcagaacg aggtctcgcg
     1441 cagcatcgaa gtgatgcgca aggcgctggt caccaagaac acggaaatcg aaaccgtctc
     1501 gcgcgatttc gcgcgctggg acgacatgta ttcgtacgtc tcctcgctcg atcccaactt
     1561 cgagtaccag aacttctccg aagccggcct cgacgaaatg aacgtcgaca tggtctggct
     1621 gctcgacccg gccgagaagc tgctctcgtc tttcgagaac gataccgacg atgcccaata
     1681 tcgccatccg gccagcgacg ccgtcgtggc cgagatcaac cggctgacgc cgatcgtgcg
     1741 caccgtgctc gaccaggaag gcacgctgcg gctggtgctg atcaacggtg ttccgcacgt
     1801 catcgccgcc cataccgtgc ttcgcagtga ccgcagcggt ccggccggcg gcacgttggt
     1861 attcgcgcgc cgcatcggcg cgccggaagt tgcaagcgtt ggcgccgata cgcagctcga
     1921 tgtgaagttc gccctgctcg acgaccgcgc catggccgaa gacaaggcgg aactgctggt
     1981 tgcacccgac ggccggcgga tccttcggcg ttccgacaac ttcatccagg gccagttgcg
     2041 cctcgattcg atcgacaacc gtcccctcgc cgtgctgtac acgacgctgc cgcgcaccgt
     2101 catgcagaac ggcaaacaag gtgtgagcta cctcgtcggc tgggtcgcgc tgctcatcag
     2161 catcgtggtc gccgcctggc atgcctacag cggccggctg cgtcgtacat cggcagccgc
     2221 ggccaccagc gagacccgct atcgggccat cttcgatcgc gccgcgtccg gcatcgtgtt
     2281 gttcgacccg cattcgcgcc ggattctcga tgcgaacccg caggcgcaac gactcatcga
     2341 cgcgtcgctc gacgaactgc ggcgccgcga cgtcgcgacc atcttcgaca cgccggtttc
     2401 cctgtcggcc ggcgaccatg aacaaccggc cgagaaccgt ccggcgcagc tcattcgcgg
     2461 cgatgacgag cgtcgtgacg tggagttctc ggtcgtcgat ctggatgccg gcacgggccg
     2521 cgtccacgcg atgctgctac aggacatctc gcaccggcgt gaagccgagc gccgcgctca
     2581 ggaacatcag ttgtcgctgc agcacctcgc cacgcacgat gcgctgacgg ggcttccgaa
     2641 ccgcacgttc ctcaacgagg aactgtcgcg cctcatcgac gaagccgccc gccgcggcga
     2701 ttcgctgtcc atcctttaca tcgactgcga caacttcaag aacatcaacg actcgcgcgg
     2761 ccactcgatc ggcgacgact accttcgtgc ggtcgcacgg cgactgcgcg attccatcgc
     2821 cagccccgac ctcgtggtcc gcatgggtgg tgacgaattc ctcgtcgtca cgcgccacta
     2881 cggcctggag cccgacagcg cggcgatagc cgagcgcgta gccgcggcgc tgaagaagcc
     2941 ggtgcgcctt ggtgaagcca cctactccgc cacgacgagc attggactgt cggtctaccc
     3001 gcgcgacgcc accacgatga gcgagctgct gcgttcggcc gacatcgcgc tgtacgaagc
     3061 caaggcgcgc ggccgcgaca acgttcagat gttcgactcg gccatgaaca agcgcgtgca
     3121 cacgcgcctg gcgctggagc aggacctgcg cagcgcggtc aaccgcgagg ccatcgagat
     3181 ccagctgcaa cccatcgtcg atattcgcag tggccggctg gtcagtttcg aagcgctggc
     3241 gcgctggaac gacccccgtc atggttctgt gccgccgttg cagttcatcg aagtggccga
     3301 agcatccgat ctcatcgtcg aactcggcga atgcgtgttc aggaacgtcg cgcggcagat
     3361 cgccgcgtgg caacgcgccg gtctcaagcc ggttccggtg gccgtgaacg tctccgccaa
     3421 gcagctcaag cgttccaatc tccccgagcg cctcgtggcc atcgcccacg aattcgacat
     3481 cgcgcctcac cagatggaag tggaactcac tgaaagcgtg gcgatgcagg attcggagca
     3541 gcacgtgacc aagcttcacg cactgcgcga tctcggcgtg cgtgtgtcgg tggatgactt
     3601 cggcacgggc tattcgagcc tcgcgtacct gcgcaacctg cccatcgact atctcaagat
     3661 cgaccgcacg ttcgtgcgcg acatgaacgt cgatcgcaat gacgccgcga tcgtgcgcgc
     3721 catcatcagc atggcgcaga gcctgcacct cggcacggtg gcggaaggta tcgagacacg
     3781 cgagcacgcc tcgcagttgc tggcgctcgg ctgctcgacc ggtcagggat acttcttcgc
     3841 caaaccgatg gcggcgcacg aaaccaacga actgctgcgc acgatgcgcg gcgccgcgcc
     3901 cgtgaccacg ggcccgagcc tggagatcgt cagggtttcc tgaagtgctg gctagggctt
     3961 gccgggcacc cagaagtctt tccaacccag gcccgcatcc gtgatggcct gcagccggcg
     4021 cttcaactgc gcctcgtcga aactcgtgac gccacccgag ctgaacgcga cgcggtagcc
     4081 acgcgccttg aggctcttga gtagttcgac atccggtccg cggtcacgat ccacgagcac
     4141 ggcgaccgag ccgccgatgt tgggtatctg ctggatggcc agcggcaaca cgccgtagcg
     4201 ccgtgcctgg ctccagaatt cgtcagcgcc gttcgccggc gccgacattc cgaggtcgat
     4261 gagcgggatg ttgtcgttcg acgcctgcgt gatgagctga tcgagcgcgg aatcggcgag
     4321 cggcacgagg ccggcgggcg gcgtggcgtc attgggcagg atccgcactt tcatgttgcg
     4381 gtacttcacg acgctcgcgg gatcgtgcgc ctgcagcgcg aacgtgcccg agctcagcaa
     4441 ccgtcccttc ttgtccttcg ggcgaaacgc gtcggccggt tcggcgtatt cgcagatcaa
     4501 ctgatcgttg atgaaggtct ggatggtctt gccgacgacg cggatgcggt agttgaacca
     4561 gacgttgtcc gcgacgggcg cctgccaggt gttgcgcacg gcgtagacgc tggcggtcat
     4621 cttgtattcg atgtagccgt tcatcgtcga tgccggcgga ttggaattga tgacctgcag
     4681 ctcgtagccg gcttcgggcc aacccgggcc ttgccacttc gtgtgcacgt agatgccgct
     4741 attggcgccc ggcgaagtca tgacttcggc ctggaactcg aaattcctga aatcgtgttt
     4801 gccgaccttg ccgacgtaga acaggtgcga acggtcgccg cgcgaaacga atgcgccgtc
     4861 ttcgacgacc caggattcgg gacgctcggc gggtttccag ccggccaggg acttgccgtc
     4921 ctgcaatgca atccagtctt gcgccagcac cagcggcgac gcacccagtg caacgacgcc
     4981 cacgaccaac accgcgaatc gcttcatggc cactccccca ttcttgtgcc cgagcctcag
     5041 ctagacgtag cgcacttcca tcacttcgta actgtgcttg ccgcccggtg caaccacgtc
     5101 gaccacgtca cccactgtct tgccgacgat ggcgcgcgcg atcggcgagg tgatggagat
     5161 tcgtccggtt ttgatgtcgg cttcgtcttc gccgacgatc tggtaggaga tgcgcttgcc
     5221 gtcgtcctga tcttcgagat cgacggtggc gccgaagatc accttgccgg tattggtgag
     5281 gccggcggtg tcgatgactt ctgctgtcga cagcttggcc tcgatctcgc caatgcgccc
     5341 ttcgatgaag ccctgctgct cgcgcgcggc gtggtattcg gcgttctcgg agagatcgcc
     5401 atggctgcgc gcctcggcga tcgccttgat gacgcgcgga cggtcctcgc ccttgagccg
     5461 cttcagttca ttgcgcaacg cctcggcgcc acgcaacgtc atcggggtgc gtttcatgac
     5521 cttgcctctt tgtgcaggtc ctgtagccgg ttcacttcga cggcgtccag gtgatcgaga
     5581 gcttcgcagg ttgcgagcgc gcccgcgaga gtcgtgtagt acgtaacgcg cttgtgcact
     5641 gcttcgcgac gaatcgaatt cgattccagg atggcagtct tgccttccgt cgtgttcacg
     5701 atcatggcga actcgtcgtt cttgatcatg tcgacgatgt gagggcgccc ttctcgcacc
     5761 ttgttggcgc gtttgcaggc aaacccggca tcggtgatgg ccttcgcggt tccctcggtt
     5821 gccgcgagct cgaagccgtg ctcgatgagt ttcttcgcca gcacgaccgc gccggctttg
     5881 tcacgctcgc gcacgctgat gaggaccact ccggatcgcg gcagcgtgac gcccgaggcg
     5941 gcctgggcct tggcataggc ttcgccaaac gtgcggcccg tgcccatgac ttcgcccgtg
     6001 ctcttcatct cgggacccag gatcgggtcg gactcgggga acttgttgaa cggaaacacc
     6061 gcttccttca ccgagaagta aggcggcacg acttcggcga tgttctccat gttcttcatc
     6121 ttctttccgg tcatgacgcg cgcgccgatc ttggcgagct gcaagcccgt ggccttcgaa
     6181 acgaacggcg cggtgcgcga cgcgcgcgga ttcacttcga gtacgtagat gatgccggcc
     6241 tggatcgcga actggatgtt catgagtccg atgacgttga gcgccttggc gagtttgcgc
     6301 gtctgttcgc gcagctcggc ctggatttcc ggcgacaggc tgttgggcgg caacgagcat
     6361 cctgaatcgc cggagtgcac tccggcctgt tccacgtgtt ccatgatgcc gccgatcaac
     6421 acgtcttcgc cgtcacaaca cgcgtcgacg tcgacttcga tggcgacatc gaggaatcga
     6481 tcgagcagca ccgggctgtc gttcgacacc ttgaccgcgt gcgtcatgta gttgcgcagg
     6541 tcttcctcat tgaagaccac ttccatcgcg cggccgccga gcacatacga gggccgaacg
     6601 accagcggga aacccacggt gcgcgcgagc gcgaccgcct ggtcttcggt acgcgccgtg
     6661 gcgttggccg gctgtttgag ccccaggttc ttgacgagca cctggaagcg ctcgcggtct
     6721 tcggccatgt cgatggaatc cggcgaggtg ccgatgatcg gcgcgcctgc ggcttcgaga
     6781 gcacgcgaca gcttgagcgg cgtctgaccg ccgtactgca cgatgacacc ttcaggctgt
     6841 tccttggcga tgacttcaag cacgtcctcg agcgtgaggg gttcgaagta aagccggtcg
     6901 gaggtgtcgt agtcggtgga gaccgtctcc gggttgcagt tgaccatgat ggtttcgaag
     6961 ccgtcttcgc gcagctgcag cgcggcgtgc acgcagcagt agtcgaattc gatgccctgg
     7021 ccaatgcggt tcggaccacc acccaggatc atgatcttgc gcttcttggt cggctgcgct
     7081 tcgcactctt cttcgtaggt cgaatataga taggccgtgg acgtggcgaa ctccgcggcg
     7141 caggtgtcga cacgcttgaa caccggccgg atgttcagcg cgtgacgctt ggcgcggatg
     7201 gcggcctcgc cggcgcccgt gagcgttccc aggcggctgt ccgagaagcc cttgcgcttg
     7261 aggtcgcgca ggcgatcggt ctcgagggcc gccacgccgc cttcgcgaac gctcgcctct
     7321 tcgatgatga gatcttcgat ctgcgcgagg aaccacggat cgatgtacga aagctcttcg
     7381 atgcgctcgc gcgaccagcc ggcgcggaat gcgtcggcga cgtacaacat gcggttcggt
     7441 cccggagcac gcagctcgta ggcgagtttg tcgcgatcgt cttcggcgag cggcatttcc
     7501 ttgaactgct cgttgagacc gtcgagaccg gtttccagac cgcgcagcgc tttctgcagc
     7561 gattcctgga aggtgcggcc gatggccatc acctcaccca cggatttcat ctgcgtggtg
     7621 agccgcgagt tggcggcggg aaatttttcg aacgtgaagc gcggaatctt ggtgaccacg
     7681 tagtcgatgc tgggctcgaa cgacgcgggc gtggcaccgc cggtgatgtc gttcttgagc
     7741 tcatcgagcg tgtagccgac cgcgagcttc gcggcgatct tcgcgatcgg aaatccggtg
     7801 gccttggagg ccagcgccga cgagcgcgag acgcgcggat tcatttcgat gaccagcagc
     7861 cgcccgtctt tcgggttcac cgcgaactgc acgttcgaac cgccggtatc gacgccgatc
     7921 ttgcgcagca ccgcgatcga cgcgttgcgc atgcgctggt attccttgtc cgtgagcgtc
     7981 tgcgccggcg cgacggtgat ggagtcgccg gtatgcacgc ccatcggatc gaagttctcg
     8041 atcgagcaca cgatgatgca gttgtcgttc ttgtcgcgaa cgacctccat ctcgaattcc
     8101 ttccagccga tcaccgactc ttcgatgagg acttcggaag tcggcgaggc atcgaggccg
     8161 cgctcgacga tctgctcgaa ctcttcacgg ttgtaggcga tgccaccacc cgagccaccc
     8221 atggtgaacg acggacggat cacgcaggga aagccgagtc cctgctgaat ctccatggcc
     8281 tgcggcaagg tatgcgccac cccggcccgc ggacactcga ggccgatttc gcgcatggcg
     8341 ttgcggaaca gctcgcggtc ctcggccatg tcgatggctt cgcgcgacgc ggcgatgagt
     8401 tcgacgccgt ggcgcgccag tacgccttca cgcacgaggt cgagcgcggt gttgagcgcc
     8461 gtctggccgc ccatggtggg cagcagtgcg tcgggctctt ccttctcgat gattcgcgcc
     8521 agcgtgcgcc agttgatggg ctcgatgtag atcgagtcgg ccatctccgg gtcggtcatg
     8581 atcgtcgccg gattcgagtt cacgagaatg acccggtaac cctcctcgcg cagcgccttg
     8641 caggcctggg cgccggagta atcgaattcg cacgcctggc cgatgatgat cgggccggcg
     8701 ccaatgatca ggatgctttt gatgtcagta cgttttggca ttagtgctca gcgtgtggct
     8761 caggccgctt cattcggtgg tggccggcgc gggccgcgca acttggcctg gagccggcgc
     8821 agcatgaggt gtacgaagcg gtcgaacatg gcgcccattt cctgcgggcc cgggctcgct
     8881 tccggatggc cctggaaact gaacgccggc ttgtcgatgc gggctatgcc ttgcagcgaa
     8941 ccgtcgaaca gcgaccgatg tgtcgccttg agcgtggacg gcagatcgct ttcatccacc
     9001 gcgaagccgt ggttctgact ggtgatgaag acacggccgg tctccagatc ctgcaccggg
     9061 tggttcgcgc cgtggtggcc gaatttcatc ttcatggtgc gcgcgccgct cgccagtcct
     9121 aacaactggt gaccgaggca gatgccgaaa gtgggcagtt ccgcgacgac cagcgcgcgg
     9181 atcgcctcga tggcgtaatc gcagggttcg ggatcacccg gcccgttcga caggaacacg
     9241 ccatcgggct cgagcgcgag gacctcttcg gccgtggtct gcgcggggac gaccgtcatg
     9301 cggcaaccgc gatcggacaa catgcgcagg atgttgcgct tcacgccgta atcgaacgcg
     9361 accacgtgca gtcgctggtg cgcgcgcatc acggggcggt tt
//
LOCUS       DATDEU010000629         7591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1582364, whole genome shotgun sequence.
ACCESSION   DATDEU010000629 DATDEU010000000
VERSION     DATDEU010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582364"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VIV63_15050"
     CDS             complement(<1..228)
                     /locus_tag="VIV63_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY6125968.1"
                     /translation="MATIFEKIIAGEIPATIVHQDDRVTAFRDISPQAPVHVLIIPNK
                     PIPTVNDVTEDDERTLGHMFVVARDLARAEGI"
     gene            complement(251..754)
                     /locus_tag="VIV63_15055"
     CDS             complement(251..754)
                     /locus_tag="VIV63_15055"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HEY6125969.1"
                     /translation="MRLKVLLLSVVAGAAFAAEPAGVMTLEWTDLIPLNARGEQSAAP
                     PAASHDYLGESGPAAVQQSAAAPVNMELDGKPVKIPGFIVPLDVAKDGTVSEFFLVPY
                     FGACIHVPPPPPNQIVYVTTKKGIALDSIYEAYWITGTMKMQNKSTRLGAAAYSLAAD
                     KVEIYKY"
     gene            complement(756..1955)
                     /locus_tag="VIV63_15060"
     CDS             complement(756..1955)
                     /locus_tag="VIV63_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6125970.1"
                     /translation="MSGTLQLALRSLANRRTTVLLTLAAIAVSVSLLLGVQKLRSAAR
                     DGFTSTVSGVDLIVGARSGPLNLLLYSVFRVGDATANVSWDSYQLVANHRDVAWTIPI
                     SLGDSHRGFRVLGTTDAYLEHYRIAGGRPLAIREGKWFADTHDVVLGADVAEELRYST
                     GQPIVIAHGAADVSFAQHDDHPFVVAGILARTGTPVDRTVHLSLEAMAALHEESAPKS
                     LTAFMVGMRDRTALLTMQRALNEYRKEPLLAIIPAVALKQLWDLIGVADRALMIVAAF
                     VVFAGLLGMLTTILVTLNERRREMAILRSVGAGPSHIFTLMVAEAGILASAGVLAGIA
                     ITYALLAAAQPLLAARYGIFVPLAGLTVSDAAMLAGIVVAALLMGVLPAWRAYRNTLS
                     DGLTIRV"
     gene            complement(1952..2611)
                     /locus_tag="VIV63_15065"
     CDS             complement(1952..2611)
                     /locus_tag="VIV63_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6125971.1"
                     /translation="MKFSWPGAPVLLEIERLQVERGERVFLRGPSGSGKSTLLGLIGG
                     VLAPASGSVRVLDQPLHEMSAAQRDRFRGEHVGFVFQMFNLIPYLSVRENVLLALRFA
                     PARAGRIHPRDRNAEADRLLAATGLDDAALLERPVTQLSIGQQQRVAAARALLGGPEL
                     LVADEPTSALDHDARETFLKLLMRECAAHGTTLLFVSHDPTLGTLFDRELRLDEINTA
                     A"
     gene            2722..3738
                     /gene="holA"
                     /locus_tag="VIV63_15070"
     CDS             2722..3738
                     /gene="holA"
                     /locus_tag="VIV63_15070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY6125972.1"
                     /translation="MKLSLETLRAHLAGEPARAYLVSGDEVLLVGEAADAIRARAKRA
                     GFDEREVHFIERVADWDAVRSASNNLSLFGSRKLIEIRLPSGKPGVGGGNAIVDLLEN
                     VSPDNISLMITGKLEREQNSSAWVKAFESVGAWLPTSPVDLARLPQWLRARAATLELD
                     LADDAVRFIVERTEGNLLAAQQELDKLALITLDKGKGKRVDLAAVQASIGDSARFDVF
                     QLGEAALGGDVPRALRILAGLRSEGVEAPLALWSISREIRSVWGTTQQGPSRGWQRPS
                     AALENAKRRAAKLPYARLAARISRADRMIKGQHPGDAWDEMALIVVEFAGRRSLPLTG
                     TRAA"
     gene            3735..4388
                     /gene="nadD"
                     /locus_tag="VIV63_15075"
     CDS             3735..4388
                     /gene="nadD"
                     /locus_tag="VIV63_15075"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY6125973.1"
                     /translation="MNTPPGPIGLFGGTFDPIHYGHLRTAFELWQSLKLTEVRFLPTG
                     NPPHRERTLADAQLRVAMVRAAVADQPAFVVDDREVRRAGASYTVDTLTELRQENPQR
                     SLCLLLGMDAFLGLPNWHRWRELFDLAHIIVAHRPGWKAPTQGPLGELMVDRGTGAVT
                     DLHKAQAGRIYVHAVTQLEISSTELRQIIDAGRDPRFLVPDAVREIIRESGCYARPR"
     gene            4406..4906
                     /gene="rsfS"
                     /locus_tag="VIV63_15080"
     CDS             4406..4906
                     /gene="rsfS"
                     /locus_tag="VIV63_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY6125974.1"
                     /translation="MATKTASRRPAAQPKNSIPQTLLRIVNSALDDMKAVNVKVMDVR
                     HLTDITDIVVIASGNSDRHVKSIADRVLQFAKQNDFSPIGVEGAREGEWVLVDLNDIV
                     VHVMLPRVREFYGLESLWEDVAPPAPEPTAVPTPAAPKAKAKAKAKSRPPAARRKPSA
                     RARAAR"
     gene            4918..5358
                     /gene="rlmH"
                     /locus_tag="VIV63_15085"
     CDS             4918..5358
                     /gene="rlmH"
                     /locus_tag="VIV63_15085"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY6125975.1"
                     /translation="MRVRIVAVGTRMPEWVTQAYDDYTRRLRSAMRIDLDELAVGKNK
                     LDEEKRLLDRVGDDYLVTLDEHGKSLTTVELARWLAQRQQDGRNLSFVIGGPDGLGPE
                     ILRKASLRWSLSALTFPHAMVRVILAEQLYRAHSVLQNHPYHRE"
     gene            5366..6007
                     /locus_tag="VIV63_15090"
     CDS             5366..6007
                     /locus_tag="VIV63_15090"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY6125976.1"
                     /translation="MAKQEEEKQTPVLRLASASPRRRQLLDLIGVPHVVTPADIDETP
                     LPGEPPNEYVLRLAREKAEAVWARHTDLPVLAADTTVVIDDEILGKPESEAEAHAMLG
                     KLSGRDHQVHTAVALWSDQHSMLFHTSTQVCFAHLSREQIQAYWDSGEPRGKAGAYAI
                     QGLGAVFVTGISGTYTGVMGLPLFETAHLLRQAGIPVWSGKWPARIDAEEGVE"
     gene            6004..7473
                     /gene="rng"
                     /locus_tag="VIV63_15095"
     CDS             6004..7473
                     /gene="rng"
                     /locus_tag="VIV63_15095"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY6125977.1"
                     /translation="MTTEILINVAPRENRAALVENGVLTELFIERQSRRGIVSNLYKG
                     RVQRVLPGMQAAFIDVGLERTAFLHAADIAAPNPESTLVGTPNVEDIRSLVKEGDDIL
                     VQVVKDPMGTKGARLTTFVALPSRLMVFMPRGEGIGVSARIDDEAERARLKALVSEAM
                     PQQTTGGYIVRTAAQGASMEQIRDDIQYLGRLWVHVKERGMLAHAGDLVYEDLPLPTR
                     ILRDELARGVDRVLVDNAASHARMLEFAAGFMPGSTTSIDLYTGQRPIFDLHGVEEEI
                     GKALERSVNLKSGGHLVIDQTESMTTIDVNTGGFVGHRNLEDTIFRTNLEAAVAIARQ
                     LRLRNLGGIIIIDFIDMADAAHRTQVMEALQRALAGDHAQTHIASVSSLGLVEMTRKR
                     TRDSLEHLLCAACPSCEGRGFVRTPETVCHEIFREIVRQSRQFQSRELLILAHQNVID
                     RLLDDESAVLAELEHQVGRPIRLQVDTLYGFDQYDVVLA"
BASE COUNT         1371 a         2491 c         2470 g         1259 t
ORIGIN      
        1 gatgccctcg gccctggcga gatcgcgcgc cacgacgaac atgtggccga gcgtgcgttc
       61 gtcgtcctcg gtgacgtcgt tgaccgtggg aatgggtttg ttaggaatga tgagcacgtg
      121 caccggcgcc tgcgggctga tgtcgcgaaa cgcggtgacg cggtcgtcct ggtggacgat
      181 ggtggcggga atctctcccg cgatgatctt ctcgaagatg gtggccatgt ctgttgaaat
      241 tccgctgcgc tcagtacttg tatatttcca ctttgtcggc cgccagggaa tacgcggcgg
      301 cgccgaggcg cgtggatttg ttctgcatct tcatggtgcc cgtgatccag tacgcctcgt
      361 aaatcgaatc gagcgcgatg ccctttttcg tcgtgacgta gacgatctgg ttaggcggcg
      421 gtggcggcac gtggatgcag gcgccaaagt agggaaccag gaagaattcg ctgaccgtgc
      481 cgtccttcgc gacgtcgagc ggcacgatga accccggaat cttcacgggc ttgccatcga
      541 gttccatgtt gaccggcgca gcggcggatt gctggaccgc cgccgggccg gactcgccga
      601 gatagtcatg cgaggccgcc ggcggcgccg ccgactgctc gccgcgcgcg ttcagcggga
      661 tgaggtcggt ccattcgagc gtcatgacgc cggccggctc ggccgcgaag gccgcgcccg
      721 cgaccacgct caacaaaaga actttcagtc gcatttcata cccggatagt taggccgtcg
      781 gacaacgtgt tgcgataggc gcgccaggca ggcagcacac ccatcaggag cgccgccacg
      841 actatacccg cgagcatggc ggcatcgctc accgtgagac cggcgagcgg cacgaagatt
      901 ccgtatcgcg ccgccagcag cggctgagcc gccgccagca gtgcataagt gatggcgatt
      961 cccgcgagca cgccggctga cgccaggatg ccggcctcgg cgaccatcaa cgtgaaaatg
     1021 tggctcgggc cggcacccac cgacctcagg atcgccatct cgcgccgcct ttcgttcaag
     1081 gtcaccagga tggtcgtgag catgcccaac aggcccgcga acaccacgaa cgcggcgacg
     1141 atcatgagcg cgcgatcggc gacgccgatg aggtcccaga gctgtttgag cgcaaccgcg
     1201 ggaatgatgg cgagcaacgg ttcctttcgg tattcattga gcgcgcgctg catcgtcagc
     1261 agcgccgtgc ggtcgcgcat gccgaccatg aacgcggtga ggctcttcgg cgcactttcc
     1321 tcatggagcg cggccatcgc ctcgagcgag aggtgcacgg tgcgatccac gggcgtgccg
     1381 gttcgcgcga gaatgccggc cacgacgaat ggatggtcgt cgtgctgcgc gaagctcacg
     1441 tcggcggcgc catgcgcgat cacgatgggc tggccggtcg agtaacgaag ttcttcggcc
     1501 acgtcggcgc ccaacaccac atcgtgcgta tcggcgaacc acttgccctc gcggatcgcg
     1561 agcggtctgc cgcctgcgat gcggtagtgt tcgagatacg catcggtggt gccgagcacg
     1621 cgaaagccgc ggtgggaatc gccgagcgaa atcggaatgg tccacgccac gtcgcggtga
     1681 ttcgccacca attggtaact gtcccagctc acattggccg tggcatcgcc cacgcggaac
     1741 accgaataga gcagcaggtt cagcggaccc gatcgcgcgc ccacgatgag gtccactccc
     1801 gagaccgtgc tggtgaatcc gtcgcgcgcg gcgctgcgca gtttctgcac ccccagcaac
     1861 agcgagacgc tgaccgcaat cgccgcgagc gtgagcagca ccgtcgtgcg ccggttggcg
     1921 aggctccgca gtgcgagttg cagcgttccg ctcatgccgc ggtattgatc tcatcgaggc
     1981 gcagttcgcg gtcgaacaac gtgccgagcg tgggatcgtg gctcacgaac agaagcgtgg
     2041 tgccgtgggc ggcgcattcg cgcatgagca gcttcaggaa tgtttcgcgc gcatcgtgat
     2101 ccagcgccga ggtcggttcg tcggccacca gcagttccgg tccgccgagc agcgcgcgag
     2161 ccgcggccac gcgttgttgc tggccgatgg aaagctgggt caccgggcgc tcgagcagcg
     2221 ccgcatcgtc aaggcccgtt gcggccagca gccggtccgc ttccgcgttt cgatcgcggg
     2281 gatgaattcg ccccgcgcgc gctggcgcga accgcagggc gagcagtacg ttttcccgca
     2341 ccgacagata ggggatgagg ttgaacatct ggaacacgaa gcccacgtgt tcgccgcgaa
     2401 accggtcgcg ctgcgccgcc gacatctcgt gcagcggctg gtcgagcacg cgcacgctgc
     2461 ccgatgcggg cgcgagtacg ccgccgatga gacccagcaa ggtgctcttg ccactgccac
     2521 tgggcccgcg caggaacacg cgttcgccgc gctcgacctg cagacgctcg atttcgagga
     2581 gcacgggcgc gcccggccag gagaatttca cgcgctctag gtctatggcc gctgcgggca
     2641 tgccgcatcc taccggacag gtggcttccg ggacggcatg tcgcccataa tcccgctggc
     2701 cgtgcagtat tccccccgcc attgaaactc tccctcgaaa ccctccgtgc gcacctcgca
     2761 ggtgagcccg cgcgcgccta tctcgtctcc ggagacgagg tcctgctcgt cggcgaagcc
     2821 gccgacgcca tccgtgcccg tgccaaacgc gcggggttcg acgagcgcga ggtccatttc
     2881 atcgagcgcg tggccgactg ggatgccgtg cgctccgcgt ctaacaatct gtcgctgttc
     2941 ggttcgcgca agctgatcga aatccggctg ccctccggca aacccggcgt gggtggtggc
     3001 aacgccatcg tcgatctgct tgaaaacgtc agtcccgaca acatctctct catgatcacc
     3061 ggcaagctcg aacgcgagca gaacagttcg gcgtgggtaa aggcgttcga gagcgttggc
     3121 gcgtggttgc ccacctcacc cgtggatctg gcacgactgc cgcaatggct gcgcgcgcgc
     3181 gccgcgactc tggaactcga ccttgcagac gacgccgtgc gtttcatcgt cgaacgcacc
     3241 gagggcaacc tgctggccgc acagcaggag ctcgacaagc ttgcgttgat caccctcgac
     3301 aaggggaagg gtaaacgcgt cgatctcgcg gcagtgcagg cttcgatcgg cgacagcgcg
     3361 cgcttcgacg tatttcagct cggcgaagca gcgctcggcg gcgacgtgcc gcgcgcactg
     3421 cgtatcctcg ccggcctgcg cagcgagggc gtcgaggcac cgctcgcact ctggtcgatc
     3481 tcgcgcgaga tccgcagcgt ctggggtacg acccagcagg gcccctcgcg cggctggcaa
     3541 cgtccgtccg cggcgctcga gaacgccaag cggcgcgcgg ccaagcttcc ctatgcgcgg
     3601 ctggcggcac gtatttcacg cgccgatcgc atgatcaagg gccagcatcc cggcgacgcc
     3661 tgggatgaaa tggcgctcat cgtcgtcgag ttcgccggcc gacgttctct tccactcacc
     3721 ggaacacgcg ccgcttgaat actcctcccg gtcccatcgg tctgttcggc ggcactttcg
     3781 acccgattca ctacgggcac ctgcgcacgg ccttcgagct ctggcagtcc ctgaagctga
     3841 ccgaagtccg tttcctgccc acgggcaatc ctccgcatcg cgaacgaacg ctggccgatg
     3901 cgcaattgcg cgtggcgatg gttcgcgcgg cggttgcgga ccagccggct ttcgtcgtcg
     3961 acgatcggga agtccggcgt gcgggggctt cctacaccgt cgataccctg accgagctgc
     4021 gccaggagaa tccgcagcgc tcgctgtgcc tgctgctggg catggacgcg ttcctgggcc
     4081 tgcctaactg gcatcgctgg cgagagctgt tcgacctcgc tcatatcatc gttgcgcacc
     4141 ggcccggctg gaaggcgccc acccagggac cgctcggcga actgatggtc gatcgcggta
     4201 ccggggcggt gactgacctg cacaaggcgc aggccggtcg catctacgta catgccgtta
     4261 cccagctcga aatatcctcg acagaattgc gccagatcat cgatgcgggc cgcgacccgc
     4321 ggttcctggt gccggacgcc gtgcgcgaga tcattcgcga gtccgggtgt tatgctcggc
     4381 cccgttaact aactcccgga gagaaatggc caccaaaacc gcttcacgcc gccccgcggc
     4441 gcagcccaag aactccatcc cgcagaccct gttgcgcatc gtcaatagcg cgctcgatga
     4501 catgaaagcc gtcaacgtca aagtcatgga cgtgcggcat ctcaccgaca tcaccgacat
     4561 cgtcgtcatc gcctccggca attccgaccg tcacgtgaaa tccatcgctg accgggttct
     4621 ccagtttgcc aagcagaacg acttcagccc catcggcgtg gaaggcgcgc gcgaaggcga
     4681 atgggtgctg gtcgatctga acgacatcgt ggtgcacgtg atgctgccgc gcgtgcgcga
     4741 gttctacggg ctcgagagcc tctgggaaga cgtcgcgccg cccgcgcctg agcccacggc
     4801 ggttccgacg cccgccgccc cgaaagcgaa agcgaaggcc aaggccaagt cgcgcccgcc
     4861 ggccgcccgc cgcaagccgt ccgcgcgagc ccgcgccgcg cgttagtccc ggaagacatg
     4921 cgcgttcgca tcgttgcggt cggaacgcgc atgcccgagt gggtcactca ggcgtacgac
     4981 gactacacgc gcaggttgcg cagcgccatg cgcatcgacc tcgacgaact cgccgtcgga
     5041 aagaacaagc tcgacgaaga aaaacgcctc ctggatcgcg tcggcgacga ctacctggtc
     5101 acgctcgacg aacacggcaa gtccctaacc accgtcgaac tagccaggtg gctggcccag
     5161 cgccagcagg acggacgcaa cctctccttc gtgatcggcg gccccgacgg cctcggcccg
     5221 gaaattctca ggaaagccag tcttcgctgg tccctgtccg cgctgacctt tccgcacgcc
     5281 atggtccggg tcatattggc ggaacagctg tatcgcgccc actcggtgct gcagaatcac
     5341 ccgtatcatc gcgagtgagg ggtcgatggc caaacaagaa gaagaaaaac agacaccggt
     5401 gctgagactg gcttccgcgt cgccgcggcg ccggcagttg ttagacctca tcggcgtgcc
     5461 gcacgtggtc acgccggcgg acatcgacga aacgcctttg ccgggcgagc ccccgaatga
     5521 atatgtactg cggctggcgc gcgagaaggc ggaagccgtc tgggcgcggc acaccgacct
     5581 gccagtactc gccgcggata ccaccgtggt gatcgacgac gagatcctgg gcaagccgga
     5641 atccgaagcc gaagcgcacg ccatgctcgg caagctgtcg ggacgggacc accaggtcca
     5701 caccgccgtc gcgctctggt cggaccaaca ttcgatgctg ttccacacgt ccacgcaggt
     5761 gtgtttcgcg cacctgtcgc gcgagcaaat ccaggcgtac tgggacagtg gcgagccgcg
     5821 aggcaaggcg ggcgcgtacg ccatccaggg actcggcgcc gtgttcgtca ccggaatctc
     5881 cggcacgtac accggcgtga tgggcctgcc gctcttcgag acggcgcacc tgctccggca
     5941 ggcgggcatt ccggtatgga gtggcaaatg gccggcaagg atcgatgcgg aagagggggt
     6001 tgagtgacga cggaaattct catcaacgtg gcgccacgcg agaaccgcgc ggcgctcgtg
     6061 gaaaacgggg tgctgacgga gctgttcatc gagcgtcaga gccgccgcgg catcgtgagc
     6121 aacctgtaca agggccgcgt gcagcgggtg ctgcccggca tgcaggccgc attcatcgac
     6181 gtcggtctcg aacgcacggc attcctgcac gccgcggaca tcgcggcgcc gaatccggag
     6241 agcacgctgg tcggcacgcc gaacgtcgag gacatccgtt ccctcgtgaa ggaaggcgac
     6301 gacatcctgg tgcaggtggt caaggacccg atgggcacca agggtgcgcg cctaaccact
     6361 ttcgtggcgc tgccctcgcg cctcatggtg ttcatgccgc gcggtgaagg catcggcgtc
     6421 tcggcgcgca tagacgacga ggccgagcgc gcgcgcctca aggcgctggt gagcgaagcg
     6481 atgccgcagc agacgaccgg tggctacatc gtgcgcaccg ccgcccaggg cgcgtcgatg
     6541 gagcagatcc gcgacgacat ccagtatctc ggccggctgt gggtgcacgt gaaagagcgg
     6601 ggcatgctcg cccatgccgg tgacctggtg tacgaagacc tgccgctgcc gacgcgcatc
     6661 ctgcgtgacg aactggcgcg cggggtcgat cgcgtgctgg tcgacaatgc cgcctcgcat
     6721 gcccgcatgc tggagttcgc cgccggcttc atgccgggca gcaccacctc gatcgacctg
     6781 tacaccgggc agcggccgat attcgacctg catggcgtcg aagaggaaat cggcaaggcg
     6841 ctggagcgca gcgtcaatct caagtccggc gggcatctcg tcatcgacca gaccgaatcg
     6901 atgaccacca tcgatgtgaa caccggcggt ttcgtcggcc accgcaatct cgaagacacc
     6961 atcttccgca ccaacctcga ggcggccgtg gccattgccc ggcaattgcg gctgcgcaac
     7021 ctcggcggaa tcatcatcat cgatttcata gacatggctg acgcggcgca ccgcacccag
     7081 gtcatggagg cgctgcagcg ggcgctggcc ggcgaccacg cgcagacgca catcgcgagc
     7141 gtctcctcgc tcggcctggt ggaaatgacc cgcaagagga cccgcgacag cctggaacat
     7201 ttgctctgtg cggcgtgtcc cagttgtgaa ggccgtggct tcgtgcgcac gcctgaaacc
     7261 gtctgccacg agatattccg tgaaattgtg cgccagagca ggcagttcca gtcccgggaa
     7321 ctgttaatac tggctcacca gaatgtgatc gaccgcctgc tggacgacga gtccgcggtg
     7381 ctggcggagc tggagcatca ggtcgggcgg ccgatacggt tgcaggtgga cacgctctac
     7441 ggtttcgacc aatacgacgt ggtgttggcc tgaagagacg ataaccgaga tgtcctggag
     7501 agatgaacga agcagccctt gagcaaaagg ggaacgaaag cggctcgcac tggctgagac
     7561 gcacgctgct ggtcacgttt tgtttgttgc t
//
LOCUS       DATDEU010000630         9579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_269059, whole genome shotgun sequence.
ACCESSION   DATDEU010000630 DATDEU010000000
VERSION     DATDEU010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269059"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VIV63_15100"
     CDS             <1..396
                     /locus_tag="VIV63_15100"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6125978.1"
                     /translation="PNYGYFEKKGVVVGYYAGEMPEERGGRNITAMTPAERTEYALSC
                     GEKIHAQYRGEFENAFSVSWAAIRFSNGANSRYESTEARRQVLGVIGQPDGPIYFAGE
                     HASWLSGWIAGAFESALRAVRLIHERASK"
     gene            425..2590
                     /locus_tag="VIV63_15105"
     CDS             425..2590
                     /locus_tag="VIV63_15105"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY6125979.1"
                     /translation="MNSKNGKRRLRTAACALTAFVGALAPMSHVTGHGTEARFVEFLD
                     WKKEHFTKIFASNRAHLEGLPSNWAEATPQVINVEGRSCIVGQLIAFDVDDKYGFDLD
                     EPVELSLTYASEYTTPFLIGYDKSGGTGVGLHQVTPAKAEKFPTLKLTLDRARFAGQA
                     TQGADIAIAAPDLGAMVVCDIKIARSNKTVVPTEFGTVKLSLRDAKTGGLVPARIGLY
                     DSTGKAPLASDKALMLQRFADDLRMLAANDRTFWPTENRQVFYADGTYESRVPVGTYE
                     LVVGRGIEYQYFKAKVEVRKDRTTDVAVSLKRYADMPAAGWYSGDSHIHVTRDEVADP
                     KIWGFVAAEDVHVGNLLEMGNIIKVYFDQPKAWGKASRFERDGHFIVSGQESPRTGFF
                     GHTVHFNINRPVHLKTEEYFLYHKVFEDVKSQGGLAGFAHMGWGSRDGGPPKMNRGLT
                     MLAPFGLVDFIEVLQGGRLEHEGWYRLLNLGYKVKPAAGTDWPYSDFPGVVRFYVNVP
                     GPFNLDAWFDAYDKGRTFVTNGPLLDFKINGRGIGDELRVKRGTKLDLVASAKMNPEL
                     DALDRLEVVVLGDVAGTQSAAGKDSVTIRKQFTAERSMWVAVRAYGARQDRRNMTIAH
                     SAPIYIVVDDEPTWKREAVPTIIAELRGQLKTMLTEPYETPITGNEAWENREVLTEQW
                     RKQRPLLKPRIDMADAAYARLLDQWTKLNGAATAAGGAK"
     gene            2587..4776
                     /locus_tag="VIV63_15110"
     CDS             2587..4776
                     /locus_tag="VIV63_15110"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY6125980.1"
                     /translation="MIRRAALLGCAAALASLSGSALGHGNEARYVDIMNWKKDRITKV
                     FVTNESHFDSLKYRASFPGRVEMVEGESCVISDLVALDVDDKYAFDIDETVSVTLTFA
                     TNYTAPFVVGWDKSGGSAQGLTEIDVKPDLAKKFSSVTLKLDRARFAGQGVQGSDLAV
                     GIQRQKNGVADGLVTLCDIKVERSNTTPAPAPLGTVKLTIKDAKTGGSVPARVGLYDA
                     TGRAPLPSDKALTLQRYADDLRMLNVNERTFWPSENRLSFYVDSDYDGAVPAGTYELV
                     VSRGLEYRVHQSKFEVKAGQTTSVNVAMERYSDMPSKGWYSGDSHIHVTRDEVADPKI
                     WGFVAAEDVHVGNLLEMGNITNVYFHQPKAWGKASRFERDGHFIVSGQEAPRAAFMGH
                     TVHFNIDRPIHLRTDEYFQYHKVFEEFQRTGGLSGFAHLGWSARGGSSARVIRGVNLL
                     APLGLVDFIEVLQGGRLVTDGWYRLLNLGIRVTPAAGTDWPYSDFPGVVRFFVKVDGP
                     FNLDKWFESFGKGRSFVTNGPTLEFTINGKTMGEELRVKRGTKLDISALARLNPDVDT
                     LDRLELVIGGDVTDTVSSGKTDSAQLRKQITADRSMWIAVRAHGGKQAANNMTVAHTA
                     PIYVVVDGEPAWNRDKVPETIAELRGQLQELLTEPYETPITGNEPWETRALLPAQWLL
                     QQPLLRPRIAMADAAYEKILVEWTKYSGAAKPIAALPGQAAADDHNH"
     gene            4786..6942
                     /locus_tag="VIV63_15115"
     CDS             4786..6942
                     /locus_tag="VIV63_15115"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY6125981.1"
                     /translation="MGQTAKSTGFAAGVVLIAATLFSPMDARAHGTDARYVQVMDWAR
                     PNLSRVFVTNRTHFAGISNSNFLPNALIRELEGKRCIVGDNIAFDVDDRFAFDIDETV
                     TLDITYAAQYTMPFVVAWDRSGGTGQGLSSRIEPPKSVTFSTSTITLDRARFAGLGTQ
                     GADFALATPDNSGITGALMICDVSIRRSNTTRAATGYGTVSLSIRDGQTGAAIPARVG
                     LYDGTGKAPLPSDQALMLQRFADDLRMVPVNERTFWPSQNRQAFYVDSDYQGRVPAGK
                     YELVVTRGPEYRVYHGSVEVKKDEVSKVSVSLRRYADMPAAGWYSGDTHIHVTRDEVS
                     DPSIWGFVAAENVHVGNLLEMGNISKVHFKQPKAWGKASRFERDGHFIVSGQEDPRTS
                     HFGHTVHLDIQRPIHLEAEDYFMYHKVFEESKRQGGVSGFAHMGWSEGSSDGTEVPRM
                     NRGMTLLAPFGQVDFIEVLQAGRLVTGGWYRLLNLGYRVTPAAGTDWPYSDLPGVVRY
                     YVNTGGAFNLDVWFDNFEAGKVFVTNGPLLEFSVNGKGIGEELRVKRGTRLSISATAR
                     MNPDVDSLDRLELVVLGDVTETAAANGKDSVKLTKTVTADRSQWIAVRAHGSRQDPRN
                     MTIAHSAPIYVVVDGEPTWNRDKAPAIVAELRKQLDSMLTEPVQQFSTNPWETRILET
                     EQWPLQRPMLKPQVARADAAYAQILEAMAKFPGAAR"
     gene            6939..8684
                     /locus_tag="VIV63_15120"
     CDS             6939..8684
                     /locus_tag="VIV63_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125982.1"
                     /translation="MTVKKRGLSLLAVGTVLMLAMVATSWLMPAVAAVEKSEADLCMV
                     PPGAEPSLPATLLAGQGAVQMPVTSASSEAHAFFLQGVAQLHSFWAREAERSFLQAAQ
                     LDPGMAMAYWGIATAAGGEHRSSWQNLRDRSGKGRGGSAKPGAAAKVERTSNGSAIDA
                     KTRAREAIDKAMSMRAAVTPRERLYIEAQWARLHPTTEDPDADFIVAMRKLVAAYPDD
                     LEAKSMLGLVMLYGYVLPAKAPRELTLEGIALLEQVIAVDPDHVGANHYLIHGYEGSA
                     TPEKGWQASELYPQLVPNIPHALHMPAHIFIQSNRVEDAVKALTAASANELMYMNADA
                     LYPPGHYGHNVHFLVHASNVLGRYNDSMEQVRLLLAFKETPRERAGDSQRTVWRQGHF
                     ALIKTLVRFERWQEILSGNVLPEYDKPEQRAWNAWATGLAQSATGEAAAAAVSLNAMR
                     SYIEMAGSGLEPLRIAALELEASIEARAGKRRASRRHFDEAALREASMLYTEPPAYPR
                     PVAEGWGHVALETGDYGTADKAYLRALAIEPGSGRAYLGRARALSALGKKTQSREMLR
                     RATLVWHYGNVVAQQ"
     gene            8744..>9579
                     /locus_tag="VIV63_15125"
     CDS             8744..>9579
                     /locus_tag="VIV63_15125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6125983.1"
                     /translation="MAALAASDEPVSTGAFSLPKIRATPDRIISLTVCTRPFRETGPR
                     IESEKLGRKTIVHNYGHGGSGWSLSWGSSQLAVRMAAATGEREVAVIGCGALGLTSAL
                     LAQRAGMKVRIYAKDLPPYTRSTFATGSWTPNSRFCTEAGASPEVVERWIWMVRHSHR
                     MYQNLLGLPGDPIEWRDSYQLSEDPFQPDQKRPRDGEPPYPELEKLVKDLKPASQELL
                     PGSHPFAMPYVRRQTNMVFNISSYARMLYSDFLIAGGEVVVREFASPGELARLKEKTL
                     INA"
BASE COUNT         1840 a         2961 c         3151 g         1627 t
ORIGIN      
        1 ccgaactacg gctacttcga aaagaaaggt gtcgtggtcg gttactacgc cggcgaaatg
       61 cccgaggagc gcggtggcag gaacatcacg gcgatgacgc cggccgagcg caccgaatac
      121 gccctcagct gcggcgagaa aatccatgcg cagtatcgcg gcgaattcga gaacgcattc
      181 tcggtgtcct gggcggcgat ccgcttcagc aacggcgcga actcgcgtta tgaatccacc
      241 gaggcgcgcc ggcaagtgct cggagtcatc ggccagcccg acgggcccat ctactttgcg
      301 ggtgaacacg ccagctggct cagcggctgg atcgccggag cattcgaatc ggcgctgcgc
      361 gccgtgcgcc tgatccacga acgggcgtcc aaatgaacaa cgaagttttt ctgggaggca
      421 gatcatgaat agcaagaatg gcaagcggcg gctgcgaact gcggcatgcg cgctgaccgc
      481 gttcgtcggt gctttggcgc cgatgtcgca tgtcaccggc cacggtaccg aggcgcgctt
      541 cgtcgagttc ctcgactgga agaaggagca cttcaccaag atcttcgcct ccaaccgcgc
      601 gcatctggaa ggcctgccga gcaactgggc ggaagcgacg ccgcaggtca tcaacgtcga
      661 aggccgctcg tgcatcgtgg gccagctcat tgcattcgac gtcgacgaca aatacggctt
      721 cgacctcgat gaacccgtgg aactgtcgct gacctatgcc tcggaataca ccacgccgtt
      781 cctgatcggc tacgacaaga gcggaggcac tggcgtcgga ctgcaccagg tgactccggc
      841 caaggccgag aagtttccga cgctgaagtt gacgctggat cgcgcgcgct tcgccggcca
      901 ggcgacccag ggcgccgaca tcgccatcgc cgctcccgat ctgggcgcga tggtggtgtg
      961 cgacatcaag attgcgcgca gtaacaagac cgtggtcccc acggaattcg gtacggtcaa
     1021 actatcgctg cgcgatgcca agaccggcgg cctggtgcca gcgcgcatcg gactgtacga
     1081 ctcgacgggc aaagcgccgc tcgcttccga caaagctctg atgctgcaac gcttcgcgga
     1141 cgatctgcgc atgctggcgg ccaacgatcg cacgttctgg ccgacggaga accgccaggt
     1201 tttctacgcc gatggcactt atgaaagtcg cgtgcccgtg ggtacctacg aactcgtcgt
     1261 cgggcgtggc atcgagtatc agtacttcaa ggcgaaggtc gaagtgcgca aagaccgcac
     1321 gaccgacgtg gcggtgagcc tcaagcgtta cgcggacatg ccggccgcgg gctggtactc
     1381 gggcgactcg cacattcacg tgacgcgtga tgaagtcgcc gatccgaaga tctggggctt
     1441 cgtggccgcc gaggacgtgc acgtcggcaa cctgctcgag atgggcaaca tcatcaaggt
     1501 ctacttcgat cagccgaagg cgtggggcaa ggccagccgc ttcgaacgtg acgggcactt
     1561 catcgtttcg ggccaggagt cgccgcgcac cggcttcttc ggacacaccg tgcacttcaa
     1621 catcaatcgc cccgtgcacc tgaagacgga ggaatacttt ctctatcaca aggtgttcga
     1681 ggacgttaaa tcgcagggag gacttgcggg tttcgcgcac atgggctggg gatcgcgcga
     1741 cggcggaccg ccgaagatga atcgcggcct cacgatgctg gcgccgttcg ggctcgtcga
     1801 tttcatcgaa gtattgcagg gcggccggct ggagcacgaa ggctggtacc ggctgctcaa
     1861 ccttggctac aaggtcaagc ccgcggcggg caccgactgg ccgtacagcg atttcccggg
     1921 agtcgtgcgc ttctacgtca acgtgcccgg gcctttcaat ctcgatgcgt ggttcgatgc
     1981 ctacgacaag ggccgcacct tcgtcaccaa tggaccgttg ctcgacttca agatcaacgg
     2041 ccgcggaatc ggcgacgaac tgcgcgtgaa gcgcggcacc aaactcgacc tcgtcgccag
     2101 cgcgaagatg aacccggagc tcgatgccct cgaccgcctg gaagtcgtcg tgctcggcga
     2161 cgtggccggc acgcagtcgg cagccggcaa ggacagcgtg acgattcgca agcagttcac
     2221 tgcggaacgc agcatgtggg tggcggtacg cgcgtatggc gcaaggcagg accgccgcaa
     2281 catgacgatc gcgcacagcg cgcctatcta catcgtcgtc gacgacgagc cgacgtggaa
     2341 gcgcgaagcg gtgccgacga tcatcgccga gctgcgcgga cagttgaaga cgatgctgac
     2401 cgagccgtac gagacgccga tcacgggcaa cgaagcctgg gagaaccgcg aggtgctgac
     2461 ggagcagtgg cgcaagcagc gtccgcttct gaagccgcgc atcgacatgg ccgacgcggc
     2521 gtacgccagg ctcctggacc agtggacgaa gctcaacggc gcggcgaccg cggcgggcgg
     2581 tgcgaaatga tccgccgcgc cgcgttgctc gggtgtgccg ctgcactggc ttccttgagc
     2641 ggcagcgcgc tcggtcacgg taacgaagcc cgttacgtcg acatcatgaa ctggaagaag
     2701 gaccgcatca ccaaggtatt cgtcaccaac gagtcgcatt tcgactcgct caagtaccgg
     2761 gcgtcgtttc ccgggcgggt cgagatggtc gagggcgagt cgtgtgtcat ctcggatctg
     2821 gttgccctcg atgtggatga caagtatgcc ttcgatatcg acgagaccgt ctcggtgacc
     2881 ctgacctttg ccactaacta cacggcgccg ttcgtggtcg gttgggacaa gagcggtgga
     2941 tcggcgcagg gcctgaccga gatcgacgtc aagccggacc tcgcgaagaa gttcagcagc
     3001 gtgaccctga agctcgatcg cgcccgattc gcgggacagg gcgtacaggg ttccgacctt
     3061 gccgttggca ttcagcgcca gaagaacggc gtggccgatg gtctcgtcac gctgtgcgac
     3121 atcaaggtgg agcgcagcaa caccacaccg gcgcccgcgc cactcggaac cgtgaagctg
     3181 acgatcaagg atgcgaaaac gggaggctcc gttccggcga gagtcggctt gtacgatgcg
     3241 accggccgtg cgccgctgcc gtcggacaag gcgctcacgc ttcagcgtta cgcggatgac
     3301 ctgcgcatgc tgaacgtcaa cgagagaacg ttctggccct cggagaatcg tctctctttc
     3361 tatgtggaca gcgactacga cggtgcggtc ccggcgggca cctacgaact cgtcgtctcg
     3421 cgtggcctcg agtatcgcgt gcaccagagc aagttcgaag tgaaagcggg gcagaccacc
     3481 agcgtcaacg tggcgatgga gcgctactcg gacatgccct cgaagggctg gtactcgggc
     3541 gattcccaca tccacgtcac gcgcgatgaa gtcgcggatc ccaagatctg gggattcgtg
     3601 gctgccgaag acgtgcatgt cggcaacctg ctcgagatgg gcaacatcac gaacgtgtat
     3661 ttccatcagc ccaaggcctg gggcaaggcg agccgcttcg agcgcgacgg gcacttcatc
     3721 gtctcgggcc aggaagcgcc gcgcgccgcg ttcatgggcc ataccgtgca cttcaacatc
     3781 gatcggccga ttcatctgcg caccgacgag tacttccagt accacaaggt gttcgaagag
     3841 tttcagcgca cgggcggatt gtcgggcttc gcacacctcg gctggtcggc acgcggcggg
     3901 agctccgcgc gcgtgatccg cggcgtcaac ctgctcgcac cgctcggcct cgtcgatttc
     3961 atagaggtat tgcagggagg ccggctggtc acggacggct ggtaccggtt gctcaatctc
     4021 ggcattcgtg tcacgccggc ggcgggcacg gactggccct attcggattt cccgggcgtg
     4081 gtccgtttct tcgtgaaggt ggacgggcct ttcaacctgg acaagtggtt cgagtctttc
     4141 ggcaagggcc gctcgttcgt gaccaatggc ccgacactcg agttcaccat caatggcaag
     4201 accatgggtg aggagttgcg cgtcaagcgt ggcaccaagc tcgacatctc ggcattggcg
     4261 cgcctgaatc ccgacgtcga tacgctcgac cggctcgagc tggtaatcgg cggcgatgtc
     4321 accgacaccg tttcctcggg caagaccgac agcgctcagt tgcgcaagca gatcaccgcc
     4381 gatcgcagca tgtggatcgc ggtgcgtgca cacggcggca aacaggcggc caacaacatg
     4441 acggtggcgc acacggcgcc tatctacgtg gtcgtcgacg gcgagcccgc ctggaaccgc
     4501 gacaaggtcc cggaaacgat cgccgaactg cgcggccagc tgcaggagct gctgaccgag
     4561 ccctacgaaa ctcccatcac cggcaacgag ccgtgggaaa cgcgggcgct gctgccggcg
     4621 caatggctgt tgcagcagcc gctgttgcgg ccgcggatcg cgatggcgga tgccgcgtac
     4681 gaaaagattc tcgtcgagtg gacgaagtac tcgggcgccg cgaagccgat agcggcattg
     4741 ccggggcagg ccgcggccga cgaccacaat cattgacgta gtcccatggg ccagacagca
     4801 aaatcgaccg ggtttgccgc gggcgtcgtt ctgatcgccg ccaccttgtt ctcgccgatg
     4861 gacgcgagag cccacggcac cgatgcgcgt tacgtgcagg tcatggactg ggcgcggccc
     4921 aacctgtcgc gcgtgttcgt gaccaatcgc acgcatttcg ccgggatcag caactccaac
     4981 ttcctgccga atgcgctcat tcgcgaactg gaaggcaagc gctgcatcgt gggtgacaac
     5041 atcgcattcg atgtcgacga tcgctttgcg ttcgacatcg acgagactgt cactctcgat
     5101 atcacctacg ccgcgcagta cacgatgccg ttcgtcgttg cctgggatcg cagcggcggc
     5161 accggacagg gactgagcag tcgaatcgag ccgccgaagt cggtcacgtt ctcgacgagc
     5221 accatcaccc tcgatcgcgc gcgcttcgcc gggcttggca cccagggtgc ggacttcgcg
     5281 ctcgcgacgc ctgacaactc cggcatcacg ggcgcgctga tgatctgcga cgtatcgatt
     5341 cgtcgcagca acaccacgcg ggcggcgacc ggatacggca cggtgtcgct cagcatcagg
     5401 gatgggcaga caggcgccgc gattccagcg cgggtcgggc tctacgacgg aaccggaaaa
     5461 gcgccgctgc catccgatca ggccctcatg ttgcaacggt tcgcggacga cctgcgcatg
     5521 gttccggtga acgagcgcac cttctggccc tcgcagaatc ggcaggcgtt ctacgtcgac
     5581 agcgactatc aggggcgagt gccggcagga aagtacgaac tcgtcgtcac acgcggtccg
     5641 gaatatcgcg tgtatcacgg cagcgtcgaa gtgaagaaag acgaggtgag caaggtcagt
     5701 gtgtcattgc ggcgttacgc cgacatgccg gcggccggct ggtactcggg cgatacccac
     5761 atccacgtga cgcgcgacga agtgtccgac ccttccatct ggggcttcgt cgccgccgag
     5821 aacgtgcacg tcggtaacct gctggagatg ggcaacatca gcaaggttca cttcaagcag
     5881 cccaaggcct ggggaaaggc gagccgcttc gagcgcgacg ggcatttcat cgtttccggc
     5941 caggaagatc cgcgcacctc gcatttcggc cacacggtgc atctcgacat ccaacggcct
     6001 atccacctcg aggccgaaga ctatttcatg tatcacaagg tcttcgagga atcgaagcgg
     6061 cagggtggtg tgtccgggtt tgcgcacatg ggctggagcg agggatcgag cgacggcact
     6121 gaagtcccgc gaatgaaccg tggcatgacg ctgctcgcgc cgttcggcca ggttgatttc
     6181 atcgaggtgc tgcaggcggg ccggctcgtg accggcggct ggtaccgttt gttgaatctg
     6241 ggctatcgcg tcacgcccgc cgccggcacg gactggcctt acagcgacct tcccggtgtc
     6301 gtgcgctact acgtcaacac gggtggggcg ttcaatctcg acgtctggtt cgacaacttc
     6361 gaggccggca aagttttcgt cacgaatgga ccgttgctgg agttctccgt caatggcaag
     6421 ggaattggcg aggagttgcg cgtcaagcgt ggcaccaggc tgtcgatttc agcgacggca
     6481 cgcatgaatc cggacgtcga cagcctcgat cggctggagc tggtcgttct cggtgatgtc
     6541 acggagaccg cggcggcgaa cggcaaggat tcggtcaagc tgacgaagac cgtgacggcc
     6601 gaccgcagcc agtggatcgc agtccgcgcc catggcagcc ggcaggatcc gcgcaacatg
     6661 acgatcgcgc actcggcgcc tatctacgtg gtggtcgacg gtgagccgac ctggaatcgc
     6721 gacaaggcgc cggccatcgt cgcggaattg cgcaaacaac tcgattccat gctgaccgag
     6781 cccgtgcagc agttttcgac caacccctgg gagacacgga tcctggaaac cgagcagtgg
     6841 ccgctacagc ggccgatgtt gaagccgcaa gtggccaggg ccgacgcggc gtacgcacaa
     6901 atcctcgagg ccatggcgaa attcccggga gcggcgcgat gacggtgaag aagcgaggtc
     6961 tgtcgttatt ggccgtcggc acggtcctga tgctggccat ggtcgccacc tcctggctga
     7021 tgcccgcggt cgcagccgta gaaaagagcg aggcggatct ttgcatggtg ccgccgggcg
     7081 cggagccgtc gttgccggcc acgttgttgg cgggtcaggg agcagtgcag atgcccgtga
     7141 ccagcgcctc gagcgaggcg cacgcatttt tcctgcaagg cgtggcgcaa ctgcattcct
     7201 tctgggcacg cgaggcggag cgctctttcc tgcaggccgc gcagctcgat cccggcatgg
     7261 cgatggctta ctggggtatt gccacggcgg cgggtggcga gcatcgctct tcgtggcaga
     7321 acctgcgcga ccgcagcggc aaggggcggg gaggctcggc caaaccgggc gcggcggcga
     7381 aagtcgagcg tacgtcgaat ggttcggcca tcgatgcgaa gactcgtgca cgcgaggcca
     7441 tcgacaaggc gatgtcgatg cgcgccgccg tcacgccgcg cgagcgcctg tacatcgagg
     7501 cgcagtgggc gcgcctccat cccaccaccg aagatcccga tgccgacttc atcgtcgcga
     7561 tgcgcaagct ggtcgcggcc tatccggacg acctcgaggc gaagtcgatg ctcggccttg
     7621 tgatgctcta cggctacgtg ttgccggcga aagcgccgcg cgagttgacg ctggaaggca
     7681 ttgcgctgct ggagcaagtg attgcggtgg atcccgatca tgtgggcgcc aaccactatc
     7741 tgatacacgg ctacgaaggc agtgcgacgc cggagaaagg ctggcaagcc agcgaactgt
     7801 atccgcaact cgtgcccaac attccccacg cgctgcacat gcccgcgcat atcttcatcc
     7861 agagcaatcg cgtcgaggat gcggtcaagg cattgaccgc ggcttccgcg aacgagctca
     7921 tgtacatgaa cgcggatgcg ctctatccac ccggacacta cgggcacaac gtccatttcc
     7981 tggttcacgc atcgaacgtg ctggggcgtt acaacgattc aatggagcag gtccggctgc
     8041 tgctggcgtt caaggaaaca ccgcgcgagc gggccggtga cagtcagcgc acggtgtggc
     8101 gccaggggca tttcgcgctc atcaagacgc tggtgcgctt cgagcgctgg caggaaatcc
     8161 tgagcggcaa tgtcctgccc gagtacgaca agccggaaca gcgggcgtgg aatgcgtggg
     8221 ccacgggact ggcgcaatcg gcaaccggcg aggcggcggc ggccgctgtt tcgctcaacg
     8281 ccatgcgttc ctacatcgaa atggcgggat cggggctcga gccgctgcgc atcgcggcgc
     8341 tcgagctcga ggcgagcatc gaggcgcgtg ccggcaagcg gcgcgccagc cggcggcact
     8401 tcgacgaagc cgcgctgcgc gaagcttcga tgctctatac ggaaccgcct gcctatccgc
     8461 ggccggtggc ggaagggtgg gggcatgtcg cgctcgaaac cggcgattac ggcacggccg
     8521 acaaggccta tctacgcgca ctcgccatcg aacccggcag cgggcgtgcg tacctcgggc
     8581 gtgcccgagc gttgtcggcg ctgggtaaaa agacccaatc ccgcgaaatg ctcaggcgcg
     8641 cgaccctggt gtggcactac ggcaatgtgg ttgcgcagca gtgatgcggc gtcgaagttt
     8701 catacaggcc atcggcggaa tcggggcggg cgcatcgctg ccaatggcgg cgttggcggc
     8761 cagcgacgaa ccggtttcca ccggcgcgtt ctcgctgccc aaaattcgcg ccacgcccga
     8821 tcgcatcatt tcgctgacgg tttgcacgcg gcccttccgc gaaaccgggc cgcgcatcga
     8881 aagcgaaaag ctcgggcgca agacgatcgt ccacaactat ggccatggcg gaagcggctg
     8941 gtccttgtcc tggggatcga gtcagttggc cgtgcgaatg gccgcggcca cgggcgagcg
     9001 ggaagtagcg gtcatcggct gcggagcgct ggggctgacc tcggcattgc tggcacaacg
     9061 cgccggcatg aaggtgcgca tctacgcgaa ggatctgccg ccttacacgc gctcgacgtt
     9121 cgccacgggt agttggacgc ccaactcccg gttctgcacg gaagcgggcg ccagtccgga
     9181 agtcgtcgaa cgatggatct ggatggtccg ccactcgcat cgcatgtatc agaatctgct
     9241 gggacttccg ggcgacccca tcgaatggcg ggacagctac caactgtcgg aggatccgtt
     9301 ccagccggac cagaagcggc cgcgcgatgg tgaaccgcct tatccggagc tggaaaagct
     9361 ggtcaaggat ctgaagcctg catcgcagga gctgctgccg ggctcacatc cgtttgcaat
     9421 gccctatgtc cgccggcaga ccaacatggt gttcaacatc tcctcgtacg cgcggatgtt
     9481 gtactccgac ttcctgatcg ccggcggcga ggtggtggtg cgggaattcg cgagccccgg
     9541 cgagttggcg aggctcaagg aaaagacact catcaacgc
//
LOCUS       DATDEU010000631         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_414941, whole genome shotgun sequence.
ACCESSION   DATDEU010000631 DATDEU010000000
VERSION     DATDEU010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414941"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VIV63_15130"
     CDS             complement(<1..674)
                     /locus_tag="VIV63_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125984.1"
                     /translation="MQQPGWYLQRLKVMSGAEVAHRLARGLRVLSQKFTAHEVPPADL
                     GARDLRFLPPYIAIAPAHLTAAAERILAGRYSFFDLEDCELGDPPQWNRDPLTQRTSG
                     DRRATAIDYRDEREVGNIKYLWEPNRHLHLPVLAQAHALTGDPRYAIAIQAQIDSWIE
                     QCPEGMGPNWISPLELGIRLINWSITWQLLGGLRSKMFASPEGAAFRDRWLDSIFRQT
                     RMIAGNL"
     gene            complement(678..2063)
                     /locus_tag="VIV63_15135"
     CDS             complement(678..2063)
                     /locus_tag="VIV63_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125985.1"
                     /translation="MNAVTSFAGYMKKHVLGQTLIRRNPFYYDRSSAVLARGEAWEYD
                     RRRAWAAQQLRHTLQLAKRTESGRSVHGGDTLATWPLLDKECLRHGLSSFTTGYKWFS
                     APATTGGTSGVPLKVVRSLEAIVFEQATIDRVIQSVGVDARTARTAVLRGDNPRDIEV
                     SPNPECEVINSGRIMTMSANAVTHTSVEHIANELEKFGPELLCAYPSALETLARFLRD
                     NGRTLSIPAVVTSSEVFRPEAWTLVQRMLGCKLADYYGQAERIAFAYATTPGEYRFQH
                     GYSHVEFIPYDGQNMPRDSQYRLYEIVGTSFWNGLLPIVRYRTGDLVRLPASWGEREL
                     EELSLGLRTFPGVLGRQQELIVCPQAVRVTGIGCVPRDIRNVIRFQIVQEAIDEARIL
                     VLATDEFSKDDAELVLANARARIPPEMNLTVEIAPRLERTPRGKTPLVVHRPPVHDVL
                     RRHGVEPLFTR"
     gene            complement(2060..>2753)
                     /locus_tag="VIV63_15140"
     CDS             complement(2060..>2753)
                     /locus_tag="VIV63_15140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6125986.1"
                     /translation="TVLIALSSQWRDFYVQECEVSPSQVVVLSNPVRVPARVPDRAGR
                     AQVQFLHLGRLGKRKGGYDLMNAFAALPESLRSRARLVLAGDGDVEGMRKLAEPFGDR
                     VQVLSWIDSNERDRLLAESDVFVLPSYAEGVPMSLLEAMAAGLPCITTPVGGIPDVFT
                     HGAEGMLVSPGDGAQLRTAMSTYIGDETARLAAGKRAYESARAFDVHTYARRLVDIYQ
                     RIAPVAEYRELA"
BASE COUNT          484 a          886 c          919 g          464 t
ORIGIN      
        1 aggttgcccg cgatcatgcg cgtctgccgg aagatggaat cgagccagcg gtcgcggaag
       61 gccgcgcctt cgggactcgc gaacatcttc gaacgcaggc caccgagcag ttgccaggtg
      121 atgctccagt tgatgagacg gatgccgagt tcgagcgggc tgatccagtt gggccccatg
      181 ccttcgggac attgctctat ccacgaatcg atctgcgcct ggatggcgat cgcataacgc
      241 gggtcgcccg tgagcgcatg cgcctgcgcc agcactggaa gatgcaggtg ccggttcggc
      301 tcccagagat acttgatgtt gccgacctcg cgttcgtcgc gatagtcgat ggcggtggcg
      361 cggcggtcgc ccgaggtcct ctgcgtcagc gggtcacggt tccactgcgg cgggtcgccg
      421 agttcgcagt cctcgagatc gaagaagctg taccggcccg cgaggatgcg ttccgcggcg
      481 gcagtcagat gcgcgggcgc aatggcgatg tacggcggca ggaaacgcag gtcgcgcgcg
      541 ccgagatcgg ctggcggcac ttcgtgcgcc gtgaacttct gcgacagcac gcgcaggccg
      601 cgcgccagcc ggtgagccac ttcggcgccc gacatcacct tgagacgctg cagataccat
      661 ccaggttgtt gcatgggtca gcgcgtgaac agcggctcga cgccgtggcg gcgaagcacg
      721 tcgtggacgg gaggacggtg cacgacgagc ggcgtcttgc cacgcggcgt gcgctccagc
      781 cgcggcgcga tctccacggt caggttcatt tccggtggaa ttctcgcgcg cgcgttggcg
      841 agcaccagtt ccgcatcgtc cttcgaaaac tcatcggtgg ccaacaccag gatccgtgct
      901 tcgtcgatgg cctcctgaac gatctggaag cggatcacgt tgcggatgtc gcgcggcacg
      961 cagccgatgc cggtgacgcg gacggcctgc ggacatacga tcagctcctg ctggcggccg
     1021 agcacacccg ggaatgtgcg aagtccgagc gacaattctt cgagctcgcg ttcgccccag
     1081 ctcgcgggca gtcgcacgag atcgccggtg cggtaccgca cgatcggcag caacccgttc
     1141 cagaacgacg tgccgacgat ttcgtacagc cggtactggc tgtcgcgcgg catgttctgt
     1201 ccgtcgtacg ggatgaactc gacatgcgag tagccatgct gaaaccggta ttcgccgggc
     1261 gtggtggcat acgcgaacgc aattcgctcc gcctggccgt agtagtcggc cagcttgcag
     1321 ccgagcatcc gctgaaccag ggtccaggct tcgggccgga acacttccga cgatgtgacc
     1381 acggcgggaa tcgacaatgt gcggccgttg tcgcgcagaa aacgcgcgag cgtctcgagc
     1441 gcgctgggat acgcacacaa caactccgga ccgaacttct cgagttcgtt ggcaatgtgc
     1501 tcgacgctgg tatgggtcac cgcgttcgca ctcatggtca tgatgcggcc ggaattgatg
     1561 acctcacact cgggattcgg cgatacctcg atgtcgcgcg gattgtcgcc gcgcagtacc
     1621 gcggttcgcg cggtgcgtgc gtcgacgccg accgactgga tcacgcggtc gatggtggcc
     1681 tgctcgaaca cgatggcctc gagcgagcgc acgaccttga gcggcacgcc gctcgttccg
     1741 ccggtggtgg ccggcgccga gaaccatttg tagccggtcg tgaacgacga gaggccgtga
     1801 cgcaggcatt ccttgtcgag cagtggccag gttgccagcg tgtctccgcc gtgcaccgaa
     1861 cgcccggact ccgtgcgctt cgcgagctgc agcgtgtgcc gcaactgctg cgcggcccac
     1921 gcgcgacgcc ggtcgtattc ccaggcttcg ccgcgcgcaa gcacggccga cgagcggtcg
     1981 taataaaaag gattgcgacg aatcagggtc tggccgagca catgcttctt catgtagccc
     2041 gcgaaggacg tcacggcgtt catgccagct cccgatattc cgcgaccggc gcaatgcgct
     2101 ggtagatgtc gaccagccga cgcgcatagg tgtgcacgtc gaaggcgcgc gcgctttcgt
     2161 aggcacgctt gccggctgcg agtcgcgccg tctcgtcgcc gatgtaggtg gacatcgccg
     2221 tgcgcaactg ggcgccatca cccggcgaga ccagcattcc ttcggctccg tgcgtgaaca
     2281 catcggggat gccgcccacc ggcgtcgtga tacacggcag gccggcggcc atcgcttcca
     2341 gcagggacat cggcacaccc tcggcatacg acggcaatac gaatacgtcg ctctcggcga
     2401 gcagccggtc gcgctcgttg gagtcgatcc acgacaatac ttgcacacga tcgccgaaag
     2461 gttccgcgag ttttcgcatg ccctcgacat caccgtcgcc ggcgagcacc aggcgggcgc
     2521 gcgagcgcag ggattcgggc aacgcggcga acgcgttcat gaggtcgtaa ccgcccttgc
     2581 gcttgccgag acgaccgagg tgcaggaact ggacctgcgc acgtccggca cgatcgggaa
     2641 cacgcgcggg gacgcgcacc ggattcgaga gcacgacgac ctgcgacggc gacacctcgc
     2701 attcctggac atagaagtcg cgccactggc tcgacagcgc gatcaacacg gtc
//
LOCUS       DATDEU010000632         8347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1147737, whole genome shotgun sequence.
ACCESSION   DATDEU010000632 DATDEU010000000
VERSION     DATDEU010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147737"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2229
                     /locus_tag="VIV63_15145"
     CDS             <1..2229
                     /locus_tag="VIV63_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY6125987.1"
                     /translation="SDAAWEVFQREFTLTARLNHPGVLRVYEPVRGADATVLPMVFAA
                     GGDLRQMRGAPYTKILPLLIDIAAALEHAHSRRIVHRDLKPSNILLDAAGRPLLADFG
                     AGASEGDNSSGPPGSPFSASPQQLAGEPARPTDDIYGLGALAYELLSGYPPFYPAFDA
                     KKIATEPVPRLKSVKPIPPRLELLVSWLLAKQGRERPPSMRHVADEMNAVLQDTLGLE
                     GSGADVASESEAAILGAAADNLTPALMADEAPMTASSVRELEEIDPGMLPQVDELRAQ
                     VVREQRTRNRRRGWTIAAVLIIATGGVAWLLPQFASQQKVDIGDLQIPAGPTESEQVA
                     EKQAEELATRHTELSKRLLELDSRAAAQWGGPDFAAARKSLDEIGGMLERRKYEGAPA
                     PLAELEKSLTAIEAKVPEALTAQLTEGKRALAAGEFENSRQAFDTALKIDPANQEATE
                     WLGKLAAASGVVPTLADAENAEAAKDLPKAQALFADVLKRNPGNIAATEGLARVKSAA
                     NENQFNAEMGAGLAALNAGRLSEARTHLERASQLRPENAEVRAALQRVTDTGSGRSTA
                     ELADRASRLVAEERWTEALAIYDEALARDPSLQFAQAGRAAVAPRAELGNRLQALIER
                     PERLAADEVRVDAERLLARARALPSQGPVIRSQISRLELLLPTFNQPVVLALESDSAT
                     EVAIQRVGLFGTFDRREVTLKPGKYTVTGKRSGFRDVRHEITVSPGQSGQTIVIRCLE
                     PI"
     gene            2251..4692
                     /locus_tag="VIV63_15150"
     CDS             2251..4692
                     /locus_tag="VIV63_15150"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY6125988.1"
                     /translation="MEPSPSFDASIVLRTAQDEVRYGARVLFDAGSGALRSDHIDAPP
                     PEAPPLAALRAVSGRWLFDALPESRVSINGVTVGGSRIINAGDVLTIAGSQLLVEEAT
                     PGTLALRRFDLVGNDTLPPVGDSVRVLPPSTEDLLIDLGEVPSIEGVVQTRSKRVEGS
                     RWNYAAWAMGALLVLVFGLFAMLEPISLDLRPADASVKSVGGFSWQSASSVFVFPGEH
                     RLSATREGYVPGEATVKVGGPVPAKALIHLVKLPGILEVDTGGVAAEISADGAPLGRV
                     PGPVKVPAGDRTLTFKAPRHLDHVERLTIAGGGEKQQLKVALKPSFGVVTISSVPAGA
                     QIEVDGKAAGITPAKVEMDAGIRRVQVSSPNLRAWTSSVVVVAGTPQSIGPIELGAAD
                     ARVTVRSVPSGAQVTTGGSFRGITPVTIDLSPGVSHAITVTRAGYAPWTRDLFAEAAK
                     ESTLDARLAALLVEVRIQGEPADAEVFVNGNPQGKAPVSLSLPASRQHLEVRKEGFNA
                     FVTDLVLAPGIARTIDFKLVNPKDIAGNSPQKVVTKPGIRLIIVPGGTFQAGTDRREQ
                     GRRPNEGAHKVTLLRPFYMGEREVTNAQFRQFRPTHNTGATGEFSLDLDKQPVARVTW
                     EDAAEFCNWLSTQEGLPPAYEPGEGGTFSLIIPVSNGYRLPTEAEWEYVARAASTGKP
                     LKYPWGASLPVVSGSANVGGSEALGLLGAAIDGHKDEFPVSAAPALFAPNPLGFYDFA
                     GNVSEWVNDRYLSFVASGAVTDPLGPTDGKSHAYRGSNWRTVATSELRFPWREGAVVA
                     SDVIGFRVARYVAPE"
     gene            4702..5094
                     /locus_tag="VIV63_15155"
     CDS             4702..5094
                     /locus_tag="VIV63_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6125989.1"
                     /translation="MRHLIGSILAISIAGVAAIAATTALLNAPLHIGPATGQTAPADG
                     SKPATQEIPDAPPPKGASAPEAPAPKGTEGAASVPAGSAADPSKPAAEGEAAEESDTD
                     PYEGIAPEDLPPDLQYDADASVSFPTNT"
     gene            5112..6467
                     /locus_tag="VIV63_15160"
     CDS             5112..6467
                     /locus_tag="VIV63_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY6125990.1"
                     /translation="MYGLHFDHHTLSIARDGELLATEPLAVETSAREPRFGRAALAAA
                     RGRPDEVSLSHWRELARNDVAARHVALEVHAHLRALSVTERVEAVVAVPADLDAAALM
                     ALRGALSAAGVDTRDFLDAATLTSAAVVDRSHYVLLDTGWRSATATRVSGGAECIFEE
                     AFVSERANLLDVYDLWLIAVAGAMVKNTRFDPLLSLDVEQRLFADLPMLAARAATEGK
                     VEAVVESDGVRFAVMVDAQLFADAAQPFYRELTRLTRNARIAGQDAALVLPAESRRWP
                     GFLPRVLELEQDGLVIVPAGLAAVAASLQTTESTVAPRLRRRVGRVAQHSLAADIEYV
                     ASASTSAARALPVTHILFGGDSMRFPEVGLVIGTDEVDNAPHIALPRAAAGVSRRHCS
                     LRREGERTVLIDHSRYGTWVNGARVRERAAVRPGDRVRVGTPGVEFTLISAASAVRAA
                     P"
     gene            6464..7558
                     /locus_tag="VIV63_15165"
     CDS             6464..7558
                     /locus_tag="VIV63_15165"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY6125991.1"
                     /translation="MKKRRAFEVFTLSFLDCICCGFGAVVLFYTIVQAQKGAQEILSL
                     DRLTGEVSKLEEEVKVGMLHLVQLRNTLEKTDEDAAAAQARALRVAAELKQRREQSST
                     YDEESLARRAHINQLKADIKSLEEGVKRLQGGALDKAPAGERVKSFRGRGNRKYISSL
                     QMKGKRILILLDTSASMMDEDIAKVIKLRNQPEPMRRAAQKWRRALDIVEWISAQLPD
                     NGQFQVIGYNSKAKPVQANTGTDWLNNNDPRAINGVLTAARAITPGDGTSLVNAFLAV
                     RTMQPEPDQIILITDGLPTQGSVPASRKFINSRDREKLFDDAMKIMEKKLPMDIVLLP
                     MKGDPLAAHAYWRLARRSSGSYVIPSRDWP"
     gene            7572..>8347
                     /locus_tag="VIV63_15170"
     CDS             7572..>8347
                     /locus_tag="VIV63_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY6125992.1"
                     /translation="MALGKRREFEVFTLSFLDCICCGFGAIILLLVLTDIGQPIVIER
                     SERNLRGQILDLQRQLFELRGETDVLNRELQGRTKVLTIEQQKAAALAGDLSKIRGEF
                     NASKGESAVTNIVETELVAAHQTLTAEMQRLLKNAPRRDPNVLAVGGIPVDSEWIIFV
                     VDTSGSMQADNWENAQNVMKEILDIYPKVKGLQILDDEGKPMFPSTKGQWLQDTPVQR
                     AKIVSQMVNWRSYSDSDPVQGIGAAVQGWWAADKKISVYV"
BASE COUNT         1455 a         2889 c         2735 g         1268 t
ORIGIN      
        1 tccgacgccg cctgggaagt gttccagcgt gaattcacgc tgaccgcgcg gctcaatcac
       61 ccgggcgtgt tgcgcgttta cgaaccggtg cgcggtgccg acgccacggt gttgcccatg
      121 gtgtttgccg caggcggcga cctccgccag atgcgcggcg ctccgtacac caagatcctg
      181 ccgctgctca tcgacatcgc ggccgcgctc gaacacgcgc actcgcgccg catcgtgcat
      241 cgcgatctca aaccctcgaa catcctgctg gacgccgccg ggcgtccact gctcgcggat
      301 ttcggcgcgg gcgcctcgga aggcgacaac tcgagcggac cgccaggctc gccgttctcc
      361 gccagtccgc agcaactcgc cggcgaaccc gcacggccca ccgacgacat ctacggcctc
      421 ggcgcgctcg cctacgaact gctctcgggc tatccaccgt tctaccccgc gttcgacgcg
      481 aagaagatcg cgaccgaacc cgtgccgcgg ctcaagtccg tgaaaccgat cccgccgcgg
      541 ctcgaactgc tggtctcctg gttgttagcc aagcagggcc gtgagcgtcc gccgtccatg
      601 cgccacgtcg ccgatgagat gaacgcggtg ctgcaggaca cgctggggct cgaaggcagt
      661 ggagccgacg tcgcctccga aagcgaagcg gcgatcctcg gcgccgccgc cgacaatctc
      721 acgccggcgc tcatggctga cgaagcgcca atgacggcga gctccgtgcg cgagctcgaa
      781 gaaatcgatc cgggcatgct gccgcaggtc gacgaactgc gcgcgcaggt ggtgcgcgag
      841 cagcgaacgc gcaaccggcg tcgcggctgg accatcgccg cggtgctgat catcgccacg
      901 ggcggcgtcg cctggttgct gccgcaattc gccagccagc agaaagtgga catcggcgat
      961 ctgcagattc ccgcgggccc cactgaatcc gaacaggtcg cggaaaagca ggccgaagaa
     1021 ctcgcgaccc gccacaccga actcagcaag cggcttctcg agctcgactc gcgcgccgcg
     1081 gcgcagtggg gcgggccgga cttcgccgcc gcgcgcaagt cgctcgacga aatcggcggc
     1141 atgctcgagc gccgcaaata cgagggtgcg cccgcgccgc tcgcggagct cgaaaaatcg
     1201 ctgaccgcca tcgaggcgaa agtgcccgaa gctctcacgg cgcagctgac cgaaggcaaa
     1261 cgcgcgctgg ccgccggcga attcgaaaat tcgcgccagg cgttcgacac cgcactcaag
     1321 atcgatccgg cgaatcagga agccaccgag tggctgggca agctcgccgc cgcgagcggc
     1381 gtggtgccca cactcgccga tgcggagaac gccgaagccg cgaaggacct gccgaaagcg
     1441 caggcactgt tcgccgacgt gctcaagcgc aaccccggca acatcgcggc caccgagggc
     1501 ctcgcccgcg tgaagagcgc cgcgaacgaa aaccagttca atgcggaaat gggcgccggg
     1561 cttgccgcgc tcaacgccgg acgtctctcc gaagcgcgta cccacctcga gcgcgcgagc
     1621 cagctacggc ccgagaacgc cgaggtccgt gccgcgctgc agcgcgttac cgacacgggc
     1681 agtggacgca gcaccgccga actcgccgat cgcgcctcgc gcctggtcgc tgaagaacgc
     1741 tggaccgaag ccctggctat ctacgatgaa gcgctggcgc gcgatccatc cctgcagttc
     1801 gcacaggcgg gccgggcggc cgtcgcgccg cgcgcggaac tcggcaatcg cctgcaggca
     1861 ctcatagaga ggcccgaacg gctcgccgcg gacgaagtgc gcgtcgacgc cgaaaggctg
     1921 ctggcgcggg cccgggcgct gccttcccag ggcccggtca ttcgctcgca gatttcgcgg
     1981 ctggaattac tgttgcccac gttcaaccag ccggtggtgc tggccctgga atcggacagc
     2041 gccaccgaag tcgccatcca gcgcgtgggt ctcttcggaa cgttcgatcg gcgagaggtc
     2101 accttgaaac ccggcaaata caccgtgact ggcaagcgca gcggcttccg cgacgtgcgc
     2161 catgagatca ccgtgtcgcc ggggcagagc gggcagacca tcgtcatccg ctgcctggaa
     2221 ccgatttagc ggcacgcccg tccaacagcc atggaaccct cgccctcgtt cgacgcgtcg
     2281 atcgtgctgc gcaccgcgca ggacgaggtg cgttacggcg cgcgcgtcct cttcgacgcg
     2341 ggttccgggg cgctgcgctc cgaccacatc gacgcgccac cgcccgaggc cccgccgctc
     2401 gcggcgctgc gcgcggtgtc cggacgctgg ctgttcgatg cgttgccgga aagccgcgtc
     2461 agcatcaacg gcgtcacggt cggcgggtcc cgcatcatca acgcgggcga cgtgctcacc
     2521 atcgccggct cgcaattgct ggtcgaagag gcgacgcccg gcacactcgc gttgcggcga
     2581 ttcgacctcg tgggcaacga cacgttgccg cccgtgggcg acagcgtgcg cgtgttgccg
     2641 ccgtcgaccg aagacctgct catcgatctc ggcgaagtgc cgagcatcga gggcgtcgtg
     2701 cagacccgct cgaagcgggt cgagggcagt cgctggaatt acgctgcctg ggccatgggc
     2761 gcgctgctcg tgctggtgtt cgggctgttc gcgatgctcg aacccatttc gctcgacctg
     2821 cgtccggccg acgcatcggt gaaatccgtc ggtggcttct cgtggcagtc ggcctccagc
     2881 gtcttcgtgt tcccgggcga acatcgcctg tcggcgacac gcgagggata tgttcccggc
     2941 gaagcgacgg tgaaggtcgg cggtcccgta ccggcgaaag cgctcatcca cctggtcaaa
     3001 ctacccggca tcctcgaagt ggacaccggc ggggtcgccg ccgagatatc cgccgacggt
     3061 gcgccgctcg gccgcgtgcc tggcccggtg aaagtcccgg ccggcgatcg cacactcacg
     3121 ttcaaggcgc ctcgccacct cgatcacgtc gagcgcctga ccatcgccgg cggcggcgag
     3181 aagcagcaac tcaaggtggc gctcaagccc tcgttcggtg tcgtcaccat cagctcggta
     3241 ccggcgggcg cgcagatcga agtcgacggc aaggccgcgg gcatcacgcc ggcgaaagtc
     3301 gaaatggacg cgggcatccg gcgcgtgcag gtgtcgtcgc ccaacctgcg agcctggacg
     3361 tcgagcgtcg tggtggttgc cggcacgccg cagtccatcg ggcccatcga actcggcgcg
     3421 gcggatgcgc gcgtcaccgt gcgttcggtg ccgtccggag cgcaggtgac caccggcgga
     3481 agtttccgcg gcatcacgcc cgtgaccatc gacctgtcgc cgggcgtctc gcatgccatc
     3541 acggtcacac gcgcgggtta cgcgccgtgg acgcgcgacc tgttcgcgga agccgcgaaa
     3601 gagtccacac tcgacgcgcg cctggcggcg ctgctcgtcg aggtgcgcat ccagggcgag
     3661 cccgccgacg cggaagtttt cgtgaacggc aatccgcagg gcaaggcgcc cgtgtcgctg
     3721 tcgctgccgg cgagccgcca gcacctcgaa gtgcgcaagg aaggtttcaa cgccttcgtc
     3781 accgacctcg tgctcgcgcc cggtatcgcg cgcaccatcg atttcaaact ggtgaacccg
     3841 aaggacatcg cgggcaattc gccgcagaag gtcgtcacca agcccggcat tcgtctcatc
     3901 atcgtgcccg gcggcacgtt ccaggcgggc accgatcgtc gcgaacaggg acggcgtccg
     3961 aacgaaggcg cgcacaaggt cacgctgttg cgtccgttct acatgggtga acgcgaggtc
     4021 accaacgcgc agttccgcca gttccgcccc acgcacaaca ccggcgccac cggcgaattc
     4081 tccctcgatc tcgacaaaca gcccgtggcg cgcgtcacct gggaagatgc cgccgagttc
     4141 tgcaactggc tgtcgacgca ggaaggtctg ccgcccgcgt acgaaccggg cgagggcgga
     4201 acgttctcgc tgatcattcc ggtgagcaat ggttaccgtc tgcctaccga ggccgagtgg
     4261 gaatacgtcg cgcgtgccgc gagcaccggc aaaccactca agtatccctg gggcgcgagc
     4321 ctgcccgtgg tatcgggttc ggcaaacgtc ggtggcagcg aagcgctcgg cttgttaggc
     4381 gccgccatcg atggtcacaa ggacgaattc cccgtctccg ccgcgcccgc gttgttcgcg
     4441 cccaatcctc tcggcttcta cgacttcgcc ggcaacgtgt ccgaatgggt caacgatcgc
     4501 tacctgtctt tcgtcgcgtc cggcgccgtc acggatccgc tgggtcccac tgacggcaag
     4561 agccatgcgt atcgcggttc caactggcgg actgtcgcga cgtcggagct gcgtttcccg
     4621 tggcgcgaag gcgccgtggt ggccagcgat gtcatcggct tccgcgtcgc gcgttacgtc
     4681 gcgccggaat aggagaaggc catgcgtcat ctcatcggca gcattctcgc catctcgatc
     4741 gccggggtcg ccgcgatcgc ggccacgacg gcgttgttga acgcgccgtt gcacatcggg
     4801 cccgccaccg gacagacggc gcccgccgac ggatcgaaac ctgcgaccca ggagattccc
     4861 gatgcgccgc cgccgaaagg cgcgagtgcg cccgaagctc ccgcacccaa gggtacggaa
     4921 ggcgccgcca gcgtgcccgc tggcagcgca gccgatccct cgaagccggc ggccgaaggc
     4981 gaagccgcgg aggagtcgga caccgatcct tacgagggca ttgcgcccga agacctgccg
     5041 ccggatctgc aatacgacgc ggatgccagc gtgagcttcc ccaccaatac ctgaccgcac
     5101 tccatatcca tatgtacggc ctccacttcg accaccacac cttgtccatc gcgcgcgatg
     5161 gcgagctgct cgcgaccgag ccgctggcgg tggagacgtc ggcgcgtgaa ccgcgcttcg
     5221 gccgtgcggc gctcgcggcc gcgcggggcc ggcccgacga agtgtcgctg tcgcactggc
     5281 gcgaactcgc gcgcaacgat gtggccgccc gccatgtcgc gctcgaagtg cacgcgcacc
     5341 tgcgcgcgtt gtcggtgacc gaacgcgtcg aagccgtggt ggccgtgccc gccgatctgg
     5401 atgcggctgc attgatggcg ttgcgcggag ccttgagcgc cgcgggcgtg gacacgcgcg
     5461 atttcctcga tgccgccacg ctgacgtccg ccgccgtcgt cgaccgcagt cactacgtgc
     5521 tgctcgacac gggctggcgc tcggccaccg cgacgcgcgt atccggcggc gccgaatgca
     5581 tcttcgaaga agcgttcgtg agcgaacggg cgaacctgct cgacgtctac gacctgtggc
     5641 tcatcgcggt ggcgggcgcc atggtgaaga acacgcgctt cgatccgctg ctcagcctcg
     5701 acgtcgagca acggctgttc gccgatctgc ccatgctggc cgcgcgtgcc gcgaccgaag
     5761 gcaaggtgga agccgtcgtc gaatccgatg gcgtgcgatt cgccgtcatg gtcgacgccc
     5821 agctcttcgc cgatgcggcg cagccgttct accgcgaact cacgcgcctc acgcgcaacg
     5881 cgcgtatcgc cgggcaggat gcagcgctgg tgttgccggc cgagtcgcgc cgctggccgg
     5941 gtttcctgcc gcgcgtgctc gagctggaac aggacggcct ggtcatcgtg cccgcgggcc
     6001 tggccgcggt cgcagcttcg ttgcagacca cggagtccac tgtcgcgcca cgactgcggc
     6061 gccgcgtcgg gcgcgtcgct cagcacagcc tggccgccga catcgaatac gtggcgtcgg
     6121 ccagcacctc cgccgcgcga gcgctgccgg tcacacacat tctcttcggc ggcgactcca
     6181 tgcggttccc cgaagtgggc ctggtgatcg gcacggatga agtcgacaac gcgccgcaca
     6241 tcgcgttgcc gcgggccgcc gcgggcgtgt ctcgcaggca ttgctcgctg cgtcgcgaag
     6301 gcgaacgcac ggtgctcatc gaccacagcc gttacggcac ctgggtcaac ggcgcgcgcg
     6361 tgcgcgagcg cgccgcggtt cgtcccggcg atcgtgttcg ggtcgggacg cccggcgtcg
     6421 agttcacgct catctcggcc gcgtccgcgg tgcgtgccgc gccatgaaga aacgtcgcgc
     6481 tttcgaagtt ttcacgctgt cgtttctcga ctgcatctgc tgcggcttcg gcgcggtggt
     6541 gctgttctat accatcgtgc aggcgcagaa gggtgcgcag gaaatcctct cgctcgatcg
     6601 gcttaccggc gaggtcagca agctcgaaga agaagtgaag gtgggcatgc tgcatctcgt
     6661 gcagctgcgc aacacgctcg agaaaaccga cgaagatgcg gccgccgcgc aggcgcgagc
     6721 gctgcgcgtc gcggcggaac tcaaacagcg ccgcgagcag tcatccacct acgacgaaga
     6781 aagcctggcg cgccgcgcgc acatcaacca gttgaaggcc gacatcaagt cgctcgaaga
     6841 aggcgtgaaa cgtctgcagg gtggcgcgtt ggacaaagcg cccgcgggcg aacgcgtgaa
     6901 gtcgttccgc ggccgcggca atcgcaaata catcagctcg ctgcagatga agggcaaacg
     6961 catcctgatc ctgctcgata cgtcggcgag catgatggac gaggacatcg ccaaggtgat
     7021 caagctgcgc aaccagcccg agcccatgcg gcgggcggcg cagaaatggc ggcgcgctct
     7081 cgacatcgtc gagtggatct cggcgcagtt gcccgacaac ggacagttcc aggtgatcgg
     7141 ctacaactcc aaggcgaagc cggtgcaggc caacaccggt actgactggc tcaacaacaa
     7201 cgatccgcgc gccatcaacg gcgtactgac tgcggcgcgc gccatcacgc ccggcgacgg
     7261 caccagtctc gtgaacgcgt tcctcgcggt gcgcacgatg cagcccgagc ccgaccagat
     7321 catcctcatc accgacggtc ttccgaccca gggctcggtg ccggcctcgc gcaagttcat
     7381 caattcgcgc gaccgcgaaa agctgttcga cgacgcgatg aagatcatgg aaaagaagct
     7441 gcccatggac atcgtattgc tgcccatgaa gggcgacccg ctcgccgcgc atgcgtactg
     7501 gcgtctcgcg cgccgaagca gcggctccta cgtcattcct tcgcgcgact ggccatgagg
     7561 aagggctaaa catggcactc ggcaaacgcc gcgaattcga agtcttcacg ctgtcgttcc
     7621 tcgactgcat ctgctgcggc ttcggcgcca tcatcctgtt gctggtgctc accgacatcg
     7681 gccagccgat cgtcatcgag cgcagcgaaa ggaatctgcg tggccagatc ctggatcttc
     7741 agcgccagtt gttcgagctg cgcggcgaaa ccgacgtgct caaccgcgag ctgcagggac
     7801 gcacgaaggt cctgacgatc gaacagcaga aggcggcggc gctcgccggc gacctctcga
     7861 agatccgcgg cgagttcaac gccagcaagg gcgagtccgc cgtcaccaac atcgtcgaaa
     7921 ccgaactcgt ggcggcacac cagactctca cggcggagat gcagcgcctg ctcaagaacg
     7981 cgccgcgccg cgacccgaac gtgctggcgg tcggcggcat cccggtggat agcgagtgga
     8041 tcatcttcgt cgtcgacacg tcgggcagca tgcaggccga caactgggag aatgcccaga
     8101 acgtcatgaa ggagatcctc gacatctatc cgaaggtgaa gggcctgcag atcctcgacg
     8161 acgagggcaa gcccatgttc ccgagcacca agggccagtg gctccaggac acgcccgtcc
     8221 agcgcgcgaa gatcgtctcg cagatggtca actggaggtc ctacagcgat tcggacccgg
     8281 tgcagggcat cggcgccgcc gtacagggct ggtgggccgc cgacaagaaa atcagcgttt
     8341 acgtcat
//
LOCUS       DATDEU010000633         5089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_269961, whole genome shotgun sequence.
ACCESSION   DATDEU010000633 DATDEU010000000
VERSION     DATDEU010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269961"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VIV63_15175"
     CDS             <1..1018
                     /locus_tag="VIV63_15175"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEY6125993.1"
                     /translation="ILSLLYKSTAEHVRLIMIDPKMLELSVYEGIPHLLAPVVTDMKH
                     AANALRWCVAEMERRYALRAALGVRNISGFNRKVKDATDSGKPIRDPVMTAAAANDPT
                     IDQRLIQDLVPLPYIVVVIDELADMMMIVGKKVEELIARLAQKARASGVHLILATQRP
                     SVDVITGLIKANIPCRIAFQVSARVDSRTILDQMGAESLLGHGDMLYLQSGTSVPTRV
                     HGAFVSDAEVHRVVEALKKSGAPNYIEDVLEGPSIPLAGISGEPAVEGAESGEQDALY
                     DEAVKIVTTERKPSISYVQRRLKIGYNRAARLLESMENAGVVGPLQPNGAREILAPAP
                     PGSE"
     gene            1015..1659
                     /gene="lolA"
                     /locus_tag="VIV63_15180"
     CDS             1015..1659
                     /gene="lolA"
                     /locus_tag="VIV63_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEY6125994.1"
                     /translation="MSKRPFRVSALLACLAFGTAVQGQTPLDSYLEHLKTLRTEFSQV
                     VTDGKGREVQNAKGKLVIVRPGRFRWELTPEGGGGNNSPQLMVADGRNLWFYDRDLEQ
                     VSVKAATSALTATPAGLLSGDGNIRELFTVAPAGRKEGLDWVLVTPKESDADFREARL
                     AFAKSELKRMVLKDKLGQTVRLDFVTSERNPPVAEAEMKFTPPSGADVIGTPVK"
     gene            1656..2042
                     /locus_tag="VIV63_15185"
     CDS             1656..2042
                     /locus_tag="VIV63_15185"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY6125995.1"
                     /translation="MNLRRVWWALGVVLLVLAMVVCLVPVPSVPAPFEWSDKTSHLVG
                     HGTLALYFAGLVPRRSWWKIFAWLLLMGVTLELAQHYMQLGREGDPRDAVANAAGAAL
                     GLLIARLGGARWPELAAWLLGRRAAQ"
     gene            2039..3403
                     /locus_tag="VIV63_15190"
     CDS             2039..3403
                     /locus_tag="VIV63_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY6125996.1"
                     /translation="MKAGGAGNAGGQPDFLRDVQPNDSLRPLADRMRPRTLDEFVGQE
                     QVLGPSKPLRQALEKGQLHSLILWGPPGTGKTTLARLIARRADADFMQLSAVMAGVKD
                     IREAVDRARTARAERGRRSVLFLDEVHRFNKAQQDTFLPYVEDGTLTFIGATTENPSF
                     EVISALLSRARVYVLRSLGVADIVKLLRRAIEDSERGLGQRHIDIDDEALVALAHGGD
                     GDARRALGMLEVAADLATEHEGRARITLEQVREVATGGRRRFDKGGEQFYDQISALHK
                     AVRGTDPDGALYWFARMLDGGCDPHYLARRIVRMAVEDIGLADPRGQQLALDAWQTFE
                     RLGSPEGDLALANAVVYLAVAPKSNAVYVAYGEAKADVEQFGTLDVPLRFRNAPTKLM
                     KGLGYGEGYQYAHDAPDAFVKGERYLPDEMPDRQYYRPVPRGLEIKIGEALARLRKSG
                     EDKS"
     gene            3400..3765
                     /locus_tag="VIV63_15195"
     CDS             3400..3765
                     /locus_tag="VIV63_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEY6125997.1"
                     /translation="MSWWLVGIGGALGSITRYGAYRLWPMSPGGFPVPTFTVNLLGSF
                     AIGLLYMYVAARGASADNARLFWMTGVLGGFTTYSAFALETALLGFSWTGVAYIVATV
                     AGCLLAAVAGLKMGTFLVS"
     gene            3775..5046
                     /gene="serS"
                     /locus_tag="VIV63_15200"
     CDS             3775..5046
                     /gene="serS"
                     /locus_tag="VIV63_15200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY6125998.1"
                     /translation="MLDSKQLRADPAAVAANLARRGFALDVAVYNALEERRKAAQIES
                     DRVRAERNANAKAVGMAKGKGEDASALLARAEELTHSMGASDAAIAQVQQELDAWLLG
                     LPNTLHESVPEGRDESANVEVRKHGTPRAFDFTPKDHVELGEKLGGLDFETAAKISGA
                     RFSIMRGGLARLHRALAQFMLDLHTREHGYTELYVPYLVSGAALQGTGQLPKVEADLF
                     RVQGEQGFYLIPTAEVPATNLVRDSIVDAKQLPMKLVAHTPCFRSEAGAAGKDTRGLI
                     RQHQFEKVELVHIVKPADSYAALEALTGHAEAVLQKLELPYRVIALCGGDIGFASAKT
                     YDIEVWLPAQNKYREISSCSNCEAFQARRMQARWRNPETGKPEPLHTLNGSGVAVGRA
                     LVAVLENYQNADGSITVPAALRPYMGGADQL"
BASE COUNT          886 a         1624 c         1724 g          855 t
ORIGIN      
        1 gatcctgtct ctgctctaca agagcaccgc ggagcacgtg cgcctgatca tgatcgatcc
       61 gaagatgctc gagctctcgg tgtacgaggg cattccgcat ctgctcgcgc ccgtggtcac
      121 ggacatgaaa cacgcggcca acgcgttgcg ctggtgcgtg gccgagatgg aacggcgcta
      181 tgcgctcagg gccgcgctcg gcgttcgcaa catctcgggc ttcaaccgca aggtgaaaga
      241 cgccaccgat tcgggcaagc cgatccgcga tccggtcatg accgcggcgg cggccaacga
      301 tcccacgatc gatcagcggc tcattcaaga tctcgtgccg ctgccgtaca tcgtggtcgt
      361 catcgacgaa ctcgccgaca tgatgatgat cgtgggcaag aaggtcgaag aactcatcgc
      421 gcgcctcgcg cagaaggcgc gtgcttcggg agtgcacctg atcctcgcga cccagcgccc
      481 gtcggtcgac gtcatcaccg gtctcatcaa ggccaacatt ccgtgccgca tcgccttcca
      541 ggtgtcggcg cgcgtcgact cgcgcaccat cctcgaccag atgggcgcgg agtccttgtt
      601 aggccatggc gacatgctgt acctgcagtc gggcacgtcg gtaccgacgc gcgtgcacgg
      661 cgctttcgtc agtgacgccg aagtccatcg cgtggtcgaa gcgctgaaga agagcggcgc
      721 accgaactac atcgaagacg tgctcgaagg ccccagcatt ccgctcgccg gaatttcggg
      781 agagcccgcg gtggagggcg ccgagagcgg cgagcaggac gcgttgtacg acgaggccgt
      841 gaagatcgtc accaccgaac gcaagccttc catctcctat gtgcagcgcc ggctcaagat
      901 cggctacaac cgcgccgcgc ggctgctcga atcgatggag aacgccggcg tcgtgggccc
      961 gctgcagccg aacggcgccc gcgagattct cgcgcccgcg cccccgggaa gtgaatgagc
     1021 aaacgtccgt ttcgagtgag tgcattgttg gcgtgcctcg cattcggcac cgcggttcag
     1081 ggtcagacgc cgctcgacag ctacctcgag cacctgaaga cgctgcgcac cgagttttcg
     1141 caggtggtca ccgacggcaa gggccgcgaa gtgcagaacg ccaaaggaaa gctggtcatc
     1201 gttcgtcccg gccggttccg ctgggagctc acgcccgagg gcggcggcgg caacaactcg
     1261 ccccagctca tggtcgccga cggcaggaac ctgtggttct acgatcgcga tctcgagcag
     1321 gtgtcggtga aggccgccac cagcgcgctc accgccactc cggccggatt gttgtcaggc
     1381 gacggaaaca ttcgcgaact gttcacggtg gcgcctgccg gcagaaagga agggctcgac
     1441 tgggtgctgg tcacgccgaa ggaaagcgac gcggacttcc gtgaagcgcg gctggccttc
     1501 gcgaagtccg agctcaagcg catggtgctc aaggacaagc tgggccagac cgtgcgcctc
     1561 gatttcgtga ccagcgaacg caacccaccg gtcgccgaag cggaaatgaa gttcacgccg
     1621 ccttcgggcg cggacgtcat cggcacgccg gttaaatgaa cctgcggcgc gtgtggtggg
     1681 cgctcggcgt ggtgctgctc gtcctcgcca tggtcgtgtg cctcgtgccg gttcccagtg
     1741 tccccgcgcc cttcgagtgg agcgacaaga ccagccacct ggtcgggcac ggcacgctgg
     1801 cgctgtattt cgcgggtctg gtgccgcggc gttcctggtg gaagatcttc gcctggctat
     1861 tgctgatggg cgtcacgctc gagctcgcgc agcactacat gcagctcggc cgcgagggag
     1921 atccgcgtga cgcggtcgcg aatgcggcgg gtgctgcgct cggcctcctg atcgcgcggc
     1981 tgggcggtgc ccgctggccg gaacttgcgg catggttgtt agggcggcgg gccgcgcaat
     2041 gaaagcgggt ggcgccggca atgcgggcgg gcaacccgat ttcctgcgcg acgttcagcc
     2101 caacgattcg ctgcggcccc tggcggaccg catgcggccg cgcactctcg atgaattcgt
     2161 gggccaggag caggtgctgg gaccctcgaa gccacttcgc caggccctgg agaaaggcca
     2221 gcttcactcg ctgatccttt ggggccctcc gggcacgggc aagaccacgt tggcccgctt
     2281 gatcgcccgc cgcgccgacg cggacttcat gcagctctcg gcggtcatgg ccggggtcaa
     2341 ggacatccgc gaagccgtgg accgcgcgcg aacggcgcgc gcggaacggg gccgacgcag
     2401 cgtgctgttc ctcgatgagg tgcaccggtt caacaaggcg cagcaggaca cgttcctgcc
     2461 gtacgtcgaa gacggcacgc tcaccttcat cggcgcgacc accgagaatc cgtcgttcga
     2521 agtgatcagc gcgttgttgt cgcgggcgcg cgtgtatgtg ttgcggtcgc tgggcgtcgc
     2581 cgacatcgtc aaactactgc gccgcgcgat cgaagattca gagcgcggcc tgggtcagcg
     2641 gcacatcgac atcgacgacg aagcgctggt ggcactggcg cacggcggtg acggcgatgc
     2701 gcggcgcgcg ctcggcatgc tcgaggtggc cgcggatctc gccaccgagc acgagggccg
     2761 cgcgcggatc acgctcgaac aggtgcgcga agtggcgacc ggtggccgtc gccggttcga
     2821 caagggcggc gagcagttct acgaccagat ctcggcgctg cacaaggccg tgcgcggcac
     2881 cgatcccgac ggcgcgctct actggttcgc acgcatgctc gatggcgggt gcgaccctca
     2941 ctacctggcg cggcgcatcg tgcgcatggc cgtcgaagac atcggtctcg ccgatccgcg
     3001 cgggcagcaa ctggcgctcg acgcgtggca gactttcgag cggcttggca gtccggaagg
     3061 cgacctcgcg ctcgcgaacg cggtcgtgta cctggccgtc gcgccgaaga gcaacgcggt
     3121 gtacgtggcg tatggcgagg cgaaagccga tgtcgagcag ttcggtacgc tcgacgtgcc
     3181 gctgcgattc cgcaatgcgc cgaccaaact catgaagggt ctcggctacg gcgagggtta
     3241 tcagtacgct cacgatgcgc cggatgcctt cgtgaagggc gagcgctacc ttcccgatga
     3301 aatgccggat cggcagtact accgcccggt gccgcgcggt ctcgagatca agattggcga
     3361 agcgctggcg cgattgcgaa agtccgggga ggacaagtca tgagctggtg gctggtcgga
     3421 atcggcggtg cgctcggcag catcacgcgt tacggcgcat acagattgtg gccgatgtcg
     3481 ccgggcggct tcccggtgcc cacgttcacc gtgaatctgc tcgggtcctt cgccatcgga
     3541 ctcttgtata tgtatgttgc ggcccgtggc gccagcgccg acaatgcgcg gcttttttgg
     3601 atgacgggag tgctgggcgg gttcaccacc tactcggcat tcgcgctgga aaccgcgctg
     3661 ttgggtttct cgtggacagg cgtggcatac atcgtcgcga ctgtcgcggg gtgcctgctg
     3721 gctgccgtcg ccggattgaa aatggggaca ttcctcgttt cctaggaaga atcagtgctc
     3781 gactccaaac aacttcgtgc tgatcccgcc gccgtcgccg cgaatctcgc gcgccgcggg
     3841 ttcgcactcg acgtcgctgt ctacaatgcg ctcgaagaac gccgcaaggc cgcgcagatc
     3901 gaatccgacc gcgtgcgggc ggagcgcaat gcgaatgcca aggccgtcgg aatggcgaaa
     3961 ggcaaaggcg aagatgccag tgctctcctg gcgcgcgccg aagaactgac ccattcgatg
     4021 ggcgccagcg atgccgccat cgcgcaggtt cagcaggaac tcgatgcctg gttattgggc
     4081 ctgcctaata ccttgcacga atccgtaccc gaagggcgcg acgagtcggc gaacgtggaa
     4141 gtgcgcaaac acggcacgcc gcgcgcgttc gatttcacgc cgaaagatca cgtcgagctc
     4201 ggcgagaagc tcggtggcct cgatttcgaa accgcggcga agatttccgg cgcgcggttt
     4261 tcgatcatgc gcggtgggct cgcgcgtctg catcgcgcgc tcgcgcagtt catgctcgac
     4321 ctgcacacgc gcgaacacgg gtacaccgag ctgtacgtgc cgtacctggt gagtggcgct
     4381 gcgttgcagg gcacgggcca gttgccgaag gtcgaggcgg acctgtttcg cgtgcaggga
     4441 gagcagggct tctacctgat ccccaccgcc gaagtgcccg cgaccaacct cgtgcgcgac
     4501 agcatcgtcg atgcgaaaca gctgccgatg aaactcgtgg cgcatacgcc gtgcttccgc
     4561 tccgaggccg gcgcggccgg caaggacacg cgcgggctca tccgccagca tcagttcgag
     4621 aaggtcgagc tggtgcacat cgtgaagccc gcggattcgt atgccgcgct cgaagcgctc
     4681 acgggccacg cggaggcggt gctgcagaag ctggagctgc cgtatcgcgt gatcgcgctg
     4741 tgcggcggag acatcgggtt cgcctcggcg aagacttacg acatcgaagt gtggctgccg
     4801 gcgcagaaca aataccgcga aatttcctcg tgcagcaact gcgaagcgtt ccaggcgcgc
     4861 cgcatgcagg cgcgctggcg aaatccggag accggcaagc cggagccgtt gcatacgctc
     4921 aatggttcgg gtgtcgccgt cggtcgcgca ctcgtggccg tgctcgagaa ttatcagaac
     4981 gcggacggct cgatcacggt gcctgccgcg ctgcggcctt acatgggcgg cgccgaccag
     5041 ctctgagctc gcccatcagg cgtggatcca gaatccgctc tccgcaaac
//
LOCUS       DATDEU010000634         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1292130, whole genome shotgun sequence.
ACCESSION   DATDEU010000634 DATDEU010000000
VERSION     DATDEU010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292130"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VIV63_15205"
     CDS             <1..576
                     /locus_tag="VIV63_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY6125999.1"
                     /translation="GAFAKLKFESGVHRVQRVPDTEGSGRIHTSAATVAVLPEVAEVD
                     VNIADEDLKIDTLRSGGAGGQHVNKTESAVRMTHLPTGIVVMMQEDRSQHRNRAKAMA
                     ILRARLYDHERQRQDAARAAERRGQVGSGDRSERIRTYNFPQGRVSDHRIDLTLYKLP
                     QVIEGEALGEIIEALMTEHQAELLAAEGTVG"
     gene            618..1550
                     /gene="prmC"
                     /locus_tag="VIV63_15210"
     CDS             618..1550
                     /gene="prmC"
                     /locus_tag="VIV63_15210"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY6126000.1"
                     /translation="MSAKRTNPDATSADTMSTRPATLAGLSVEVARRRLAREFGEQNI
                     DAPALDARIIVGHVLGLDHTALMAQSGRVLAPAEADAIAALAARRHAHEPIARMLGYK
                     EFWGLPLKLNSDTLLPRPETETVVEAALAALAGEHRSARVLRIVDLGTGSGALLLALL
                     SELPNAFGIGTDISVTALGCARDNAAALGLSARASFVACDYGAALNGPVDVLVSNPPY
                     VVREEIADLQPEVRDYDPRRALDGGPDGLDGYREVAAHARRLLAPSGVLAVELGRGQL
                     DAVTAILAGAGLARPASQHDLAGISRTLVMRAAP"
     gene            2014..>2585
                     /locus_tag="VIV63_15215"
     CDS             2014..>2585
                     /locus_tag="VIV63_15215"
                     /inference="COORDINATES: protein motif:HMM:NF025137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEY6126001.1"
                     /translation="MRNGQNKRMRGRNNHRKNHNPMVRVYESNGPDVKIRGNPSHIAE
                     KYIQLARDAQTSGDPIAAENYYQHAEHYYRVIAVAQEQFRQNNPNFARDQNEMQGARD
                     DGFDDGDDDGQSGGSGDSPYGTREPQPYIPRDAQPFPQQQQHQPQRPQQGNATTEGDV
                     ERLPSFITGGQQPQQHQPHQGQQPNQNGHE"
BASE COUNT          497 a          821 c          857 g          410 t
ORIGIN      
        1 ggcgcgttcg ccaagctcaa gttcgaatcc ggcgtccacc gcgtgcagcg cgtgccggac
       61 accgaaggct ccggccgcat ccacacctcc gccgccaccg tcgcagtgtt gcccgaggtc
      121 gccgaagtcg atgtcaatat cgccgacgaa gatctcaaga ttgatacctt gcgatcaggg
      181 ggcgccggtg gccagcacgt caacaagacc gagtcggccg tgcgcatgac ccatcttccc
      241 accggcatcg tcgtcatgat gcaggaggac cgttcgcagc acaggaatcg cgccaaggcg
      301 atggcgatcc tgcgtgcccg attgtacgat cacgagcggc agcggcagga tgcggcgcgc
      361 gccgccgagc gtcgcggtca ggtcggctcc ggcgatcgct ccgaacgcat tcgcacctat
      421 aattttccgc aagggcgcgt gagcgatcac cgcattgatc tcacgctgta caagctgccg
      481 caggtgatcg agggcgaggc gctgggcgag atcatcgaag cgctcatgac cgagcaccag
      541 gcggagctgc tggcggccga gggaacggtc ggatgagcgt tcgtggcatc ctggggtgaa
      601 cccggttgac gatgcagatg tcagcgaagc gcacgaaccc ggacgcaacg tcggcggata
      661 cgatgtctac ccggcccgca acgcttgccg gtctttccgt cgaggtcgcg cgccggcgtc
      721 tggcgcgcga gttcggtgag cagaacatcg atgcgccggc gctcgatgcg cgcatcatcg
      781 tcggccacgt gctcggactc gaccacaccg cgctgatggc gcagtccggc cgcgtgctgg
      841 cacccgcgga ggccgacgcc atcgccgcgc tggcagcgcg ccggcacgca cacgaaccga
      901 tcgcgcgcat gctcggctat aaggaatttt ggggactgcc gctcaagctc aacagtgaca
      961 cgctgctgcc gcggccggaa accgagaccg tggtcgaggc ggcgttggct gctctcgccg
     1021 gcgagcatcg cagcgcgcgc gtgctgcgca tcgtcgacct cggcaccggc tcgggggcgc
     1081 tgctgctggc actgctctcg gagctgccca acgctttcgg gatcggcacg gatatcagcg
     1141 tcaccgcgct cggctgcgcg cgcgacaatg ccgcggcgct tggcctttct gcccgtgcgt
     1201 cgttcgtggc gtgcgattac ggggcagccc tcaacgggcc ggtggatgtt ctcgtctcca
     1261 atccacccta cgtggtgcgc gaggagatcg ccgacctgca gcctgaagtc cgggattacg
     1321 atcctcgccg cgcgctcgat ggcggcccgg atggtcttga tggatatcgc gaagttgccg
     1381 cgcatgcccg ccgcctgctc gcgccaagcg gggtgctggc ggtggaactc gggcggggcc
     1441 aactcgatgc cgtaaccgcg attcttgccg gcgcagggct tgcgcgaccg gcatcgcagc
     1501 atgatcttgc gggcatatct cggacgctcg ttatgcgggc ggcgccatga gtcctccaca
     1561 ttcctggtgc gaaaaaaagc gcttggattg tgggtcaaga ccgactaggt ttcggccagg
     1621 aatcgacccg agattaccga gcgccgattg cgagccccgg agcaggactg cggagagccg
     1681 acggtgcgga cgaagcgaac gaaggacgcc ggatgacccg gcagatagtt cgccaaatcg
     1741 ggacgagatg cttcatcggt tcggtgctga tccgcgggca aggcggacat gcccgtgagg
     1801 aagaggtggg caacgccgcg gtatgctgcg gtcaaggggt tgctgcttcg cgacacggaa
     1861 tctgacatcg cggcgagggc gccgtattgc tcctgcttga acgggccgga acgctgatgc
     1921 tggcgtgttg ctcgtgcgac agagtgtcga cttgtttggt gaagcacggc ggctgcagtc
     1981 cgccacaatg aaggggttgg caataggcga gtcatgagaa acggtcagaa caagcgcatg
     2041 cgcgggcgca acaaccaccg caagaatcac aatccgatgg tgcgcgtcta cgagtcgaat
     2101 gggccggatg ttaaaattcg aggaaacccg tcgcacatcg cggaaaaata cattcagctc
     2161 gcgcgcgacg cgcaaacctc gggcgatccg atcgcagcgg aaaattacta tcagcatgcc
     2221 gaacactatt accgcgtgat cgcggtggcg caggaacagt tccgccagaa caacccgaac
     2281 ttcgcccgcg atcagaacga gatgcaggga gcgcgcgacg acggtttcga cgacggcgac
     2341 gacgacggac aatcgggcgg ttcaggcgat tcaccctatg gtacgcgcga gccgcagcct
     2401 tacatcccgc gcgatgcaca accctttccg cagcagcagc aacaccagcc tcaacgtccc
     2461 cagcagggca acgcgaccac cgaaggcgat gtcgagcggc tgccgtcctt catcaccggt
     2521 gggcaacagc cgcagcagca tcagcctcac caaggccagc agccgaacca gaacggtcac
     2581 gagag
//
LOCUS       DATDEU010000635         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1000736, whole genome shotgun sequence.
ACCESSION   DATDEU010000635 DATDEU010000000
VERSION     DATDEU010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000736"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VIV63_15220"
     CDS             complement(<1..1119)
                     /locus_tag="VIV63_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126002.1"
                     /translation="MKTETPPLLVFLCGARDFHAMDWFRSARQTLPNSQLCVLTDLIA
                     GEGFKDLRCEGDRVYRLFILDPWLLRHQSSFGNVWRNLLKLVVFPLQVVLARRFNRQH
                     PGARYYCHSMYYLFLAWAAGLHYVGTPQGSEVLIRPFRSRFYKYFATRSLRAAAHVTV
                     DSRPMQQKIFEFSGVRAHVIQNGIDVQSILPYLNAQPATARKCILSIRGFTPLYRIAD
                     ILAARNRSTRHADVPVALRYPFYEATYRTQALTTLKPGDQDVGRVELADIHRLFGEAR
                     LVVSIPVSDSSPKSVYEAIFYGCAVAIAHHPYYDDLPACMKARIILVDLAQSDWLDRA
                     LDKADEIVRVRYEPSTDALTTFDKIESFKKVATLIYSET"
     gene            complement(1116..1967)
                     /locus_tag="VIV63_15225"
     CDS             complement(1116..1967)
                     /locus_tag="VIV63_15225"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6126003.1"
                     /translation="MTRIVVSGATGFVGSALTKRLRGREGTEVVALSSASGDVAEPQT
                     WHALPPAEVVVHLAARTFVPDSWTDPAAFLRTNFTGATQALEYCRKHKARLVFLSSYL
                     YGNPSRLPIDEQAPISTPNPYALSKKLAEDACRFYSESFGVEVTILRVFNIYGGGQGD
                     KFLIPLIVRQVLDGSDVHVKDLAPKRDFLFIDDLIEAIVCAVDKPQRFEIFNIASGTS
                     HSVQDVIDIVQRVAGRSVPVHSEEVRRQNEIMETLGDITKARQMLGWTPRWTIESGVQ
                     ELMRVGV"
     gene            complement(1960..2676)
                     /locus_tag="VIV63_15230"
     CDS             complement(1960..2676)
                     /locus_tag="VIV63_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY6126004.1"
                     /translation="MNSKRAIILAGGRGTRLRPYTVVLPKPLMPIGEYPILEVIIRQL
                     AHAGFSRITLAVNHQAELIKAFFGDGSKWKIKIDYSLEESPLGTMGPLRLIRDLPEDF
                     LVMNGDILSDIDYGAFHAAHVAGKNLFTISSHVRQQMVDYGVLEVGPQNTLVGFREKP
                     KSEYQVSMGIYMVSRNVLEHIPGDRPFGFDNLMLDLIKAGKKVAVQPFTGYWLDIGRP
                     DDYAQAIEQFEAMRTKFLND"
     gene            complement(2673..>3049)
                     /locus_tag="VIV63_15235"
     CDS             complement(2673..>3049)
                     /locus_tag="VIV63_15235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY6126005.1"
                     /translation="PTRDFSFVEDTVRGFIAAMESKETIGTVTNIGSGFEISIGDTAK
                     CIAGLMGAEIEIESGDSERLRPAASEVERLFASYEKAERLMQWKPAFGGREGFERGLR
                     KTIDWFTEAQNLASYKSDQYNL"
BASE COUNT          575 a          913 c          973 g          588 t
ORIGIN      
        1 cgtttccgaa tagatgaggg tcgcgacctt cttgaaggac tcgatcttgt cgaacgtggt
       61 cagcgcatcg gtcgatggtt cgtagcgaac ccgcacgatt tcatccgcct tgtccaacgc
      121 gcggtccagc cagtcggact gcgccagatc caccaggatg atgcgcgcct tcatgcaggc
      181 cggaaggtcg tcgtagtacg gatgatgagc gatcgccacg gcgcagccgt agaagatcgc
      241 ctcatagacg ctcttgggcg aagagtcgct gaccggaatc gagaccacca gccgcgcctc
      301 gccaaacaat cgatgaatgt cggcgagttc gacgcggccc acgtcctggt cgcccggctt
      361 cagagtggtt agcgcctgcg ttcggtaggt ggcttcgtag aacggataac gcagcgcgac
      421 cggcacgtcg gcgtgccgcg tcgagcgatt ccgcgcggcc aggatgtcgg cgatgcgata
      481 gagcggcgtg aaaccgcgaa tcgacagaat gcatttcctg gcggtcgccg gttgtgcgtt
      541 gagatacggc aggatcgact ggacatcgat gccgttctgg atgacatggg cacgcacgcc
      601 cgaaaactcg aagatcttct gctgcatcgg ccgcgaatcg acggttacgt gagcggccgc
      661 gcgcagggag cgcgtcgcga aatatttata gaaccgcgaa cgaaacggcc tgatgaggac
      721 ttcactgccc tgcggtgtgc cgacgtagtg cagtcccgcg gcccaggcca ggaacaggta
      781 gtacatcgag tggcagtagt accgcgcacc cggatgctgg cgattgaatc gccgggccag
      841 aacaacctgg agcgggaaca ccactagttt gagcaggttg cgccagacgt tgccgaagct
      901 cgactgatga cgcagcaacc acgggtcgag gatgaacagg cgatagacgc ggtcgccttc
      961 acagcgcagg tccttgaagc cttcgccggc gatgaggtcc gtgagcacgc agagctggct
     1021 gttgggcagc gtctgccggg cactgcgaaa ccagtccatc gcgtggaagt cgcgcgcgcc
     1081 acacaggaac acgagcaacg gcggcgtctc cgtcttcaca cgcccactcg catcaattcc
     1141 tggacgccgg attcgatggt ccagcgcggt gtccagccga gcatctgccg ggccttcgtg
     1201 atgtcaccca gcgtttccat gatctcgttc tgccggcgga cctcttcgga atgcaccggt
     1261 accgaacggc cagccacgcg ttgcacgatg tcgatgacgt cctgcacgga gtgactggtg
     1321 cccgaggcga tgttgaagat ctcgaatcgc tgcggcttgt cgaccgcgca cacgatggct
     1381 tcgatgaggt catcgatgaa caggaaatcg cgtttgggcg cgagatcctt cacgtgaacg
     1441 tcgctgccgt ccagcacctg cctgacgatc aggggaatga ggaacttgtc gccctggccg
     1501 ccaccgtaga tgttgaagac ccgcaggatc gtgacttcca cgccgaaact ttccgagtag
     1561 aagcggcacg cgtcttcggc cagtttcttg gagagtgcgt aaggattcgg cgtcgatatc
     1621 ggcgcctgtt catcgatggg cagccgcgaa gggttgccat atagatagga gctcaggaac
     1681 accaggcgcg ctttgtgctt gcggcagtat tccagcgcct gggtggcgcc cgtgaagttg
     1741 gtacgcagga acgccgcggg atccgtccag ctgtcgggta cgaaggttcg tgcagcgaga
     1801 tgaacgacga cttcggccgg cggcaacgcg tgccaggtct gaggctcggc cacgtcgccg
     1861 ctcgcgctgc tgagcgcaac cacctcggtg ccttcacgac cgcgcaaccg cttcgtgagc
     1921 gccgagccaa cgaagcccgt cgctccgctg accacgatcc tagtcattga ggaacttggt
     1981 tcgcatcgcc tcgaactgtt cgatcgcctg cgcgtagtcg tcgggccggc cgatgtcgag
     2041 ccagtaaccc gtgaagggtt gcaccgcgac cttcttgccg gccttgatga ggtcgagcat
     2101 gaggttgtcg aacccgaagg gccggtcgcc cggaatgtgt tccagcacgt tccggctcac
     2161 catgtagatg cccatgctca cctggtattc cgacttcggc ttttcgcgaa agccgaccag
     2221 cgtgttctgc gggccgactt cgagcacgcc gtagtcgacc atctgctggc ggacatgcga
     2281 ggagatcgtg aacaggtttt tcccggctac atgggccgcg tgaaacgcgc cgtagtcgat
     2341 gtcgctcagg atatcgccgt tcatgaccag gaagtcttcg ggaagatcgc ggatcaaacg
     2401 cagcggaccc atggtgccga gcggggattc ctcgagcgag tagtcgatct tgatcttcca
     2461 cttcgagcca tcgccgaaga acgccttgat gagctcggcc tggtgattga cggccagcgt
     2521 gatccgcgaa aagccggcat gcgcgagctg gcggatgatg acttcgagaa tcgggtattc
     2581 accgatcggc atcagcggct tcggcagcac gacggtgtaa ggccgcagcc gagtgccgcg
     2641 tccgccggcg aggatgatgg cgcgcttcga gttcacaggt tgtactggtc gctcttgtag
     2701 ctcgcgagat tctgggcttc agtgaaccag tcaatggtct tgcgcagccc gcgctcgaag
     2761 ccttcgcggc caccgaacgc cggtttccat tgcatgaggc gttctgcttt ttcgtagctc
     2821 gcgaacagcc gttccacttc gctggccgcg gggcgcaggc gttcggaatc gccgctttcg
     2881 atctcgatct ccgcgcccat caggccggca atacattttg ccgtgtcgcc gatggagatc
     2941 tcgaagccgc tgccgatgtt ggtgaccgtg ccgatggtct ccttgctctc catcgccgcg
     3001 atgaagccgc gcacggtatc ttcgacgaac gagaagtcgc gcgtgggat
//
LOCUS       DATDEU010000636         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1148363, whole genome shotgun sequence.
ACCESSION   DATDEU010000636 DATDEU010000000
VERSION     DATDEU010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148363"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VIV63_15240"
     CDS             <1..648
                     /locus_tag="VIV63_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126006.1"
                     /translation="NSISLGRLLPQMFYYAFAALQYKAQTPLVVSVPSGNFGNLVAGV
                     MAWKMGAPINHFSAPTTINDTVPRYFASGRVEPRTSVTTSANAMDVGNPSNLERLQWL
                     FDGDLAAMRKVISTAAHTDDEVRGSIADLYKKYSYVADPHSAIGYLGATAARRSAGAP
                     CALFLATAHPAKFREVVEPVIGKPVPLPEALAETLARKKDVERIGPTLGALSKLL"
     gene            656..2494
                     /gene="pepF"
                     /locus_tag="VIV63_15245"
     CDS             656..2494
                     /gene="pepF"
                     /locus_tag="VIV63_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEY6126007.1"
                     /translation="MPSPAPPAPSTLVPARESLDPKYTWDLSSIFSSWDAWETAFAEL
                     DHGIEAYRQYEGTLSQGSDQLLRALRDRDALAQLSYKVWYYPSLQYDEDQRNNTINAR
                     RQRVQLLIAKWQQATSWFQPELLKLPLAGVRQWMDQTAELAVYRFAIEEVFRLQEHVL
                     DERGEKLLSLTERLGSVPKDSYAALSTADAHFPTITLSTGETTQVSYGQYRKLLATCR
                     SQADRRLAYEALYDTYTASLNTYATIYNGVMQGDWFEARARGYPTTLDAALFGNAIPT
                     SVVENLIRETKLGAEPFRRYHKLRKRVLKLDDYYVFDAFVPLVDYHVKYAYEDVLDWI
                     VESVSPLGAEYQARVRRAFSERWIDVYENQGKRSGAYSAPVYGANPYMLMNYNDTLDA
                     VFTLAHELGHSMHTMLSHETQPFVYAGYTIFVAEVPSTLNEALLLNFMLTRDKTKEER
                     IVLLQHAIDGIVGTFYNQVLFADFELEAHRLVEQDQPITSETLSGIYAKLLGEYWGDA
                     LSADPRAQHTWARIPHFFQSPYYVYQYATCFASTAKLMAEIGSEDAAIRKVAVERYVR
                     LLQSGGSDHPMTLLQRAGADLRKPETVKAVSAQLNGLVDRLEQELA"
BASE COUNT          502 a          812 c          804 g          478 t
ORIGIN      
        1 aattcaatca gcctcggccg gctgctgcca cagatgttct attacgcgtt tgcggccctg
       61 cagtacaagg cgcagacgcc attggtggtt tccgttccct cgggcaactt cgggaacctt
      121 gtcgccggcg tgatggcgtg gaagatgggc gcgcccatca accacttttc tgcgccgacg
      181 acgattaacg acacggtacc gcgctacttc gcgtccggac gggtcgagcc gcggacgtcg
      241 gtgaccacgt ccgcgaacgc catggatgtc ggcaacccga gcaatctgga gcgtctgcaa
      301 tggttgtttg acggggacct ggcggcgatg cgcaaggtca tctccacggc ggcgcatacg
      361 gatgacgagg tgcgcggctc gatcgccgat ctctacaaga aatacagcta tgtcgcggac
      421 ccgcattctg cgatcggcta cctcggcgca accgcggcac gccggtcggc cggcgcgcct
      481 tgcgcacttt tcctcgcgac cgcacatccc gcgaaattcc gtgaagtcgt ggaaccggtc
      541 atcggtaaac cggtgccatt gccagaggcg cttgccgaaa ctctcgcacg caagaaagat
      601 gtcgaacgca tcgggccgac gcttggagcg ttatcgaagt tgttatgatc gacgcatgcc
      661 ttcgccagcg ccccccgcgc catcgacact tgttccggcc cgtgaatcac tcgaccctaa
      721 atacacgtgg gatctcagtt ccatcttcag cagctgggac gcgtgggaaa cggccttcgc
      781 agagctcgac cacggcatcg aagcctacag gcaatatgag ggcacgctgt cgcagggttc
      841 ggaccagctg ctccgcgcct tgcgtgatcg cgacgccctc gcgcagctga gttacaaggt
      901 ctggtattac ccctcgctgc aatacgacga ggatcaacgc aacaacacca tcaacgcccg
      961 ccgccagcgc gtgcagctgc tgattgcgaa atggcagcag gccacgtcgt ggttccagcc
     1021 ggaattgctg aagctgccgc ttgccggcgt ccgccagtgg atggaccaga cggccgagct
     1081 cgcggtctac cgcttcgcaa tcgaagaagt gttccggctg caggagcacg tgctggacga
     1141 acggggcgag aaactgttgt cgctgaccga gcggcttggc agcgtgccga aggattcgta
     1201 cgccgcgctc tcgaccgcgg acgcgcactt cccgaccatt acgctgtcga ccggtgagac
     1261 cacgcaggtc agctacggcc agtatcgcaa gctgctggcg acctgccgat cgcaggccga
     1321 tcgccgcctc gcgtacgagg cgctctacga cacttacacg gccagcctga atacttacgc
     1381 gacgatctac aacggcgtga tgcagggcga ctggttcgag gcgcgcgcgc ggggttaccc
     1441 gaccaccctc gacgcggcgc tgttcggcaa cgcgattccg acgagcgtgg tcgagaacct
     1501 gattcgcgag accaagctcg gggccgagcc gttccgccgc tatcacaagc tccgcaagcg
     1561 cgtcctgaag ctcgatgact actacgtgtt cgacgcgttc gtgccgctgg tcgactacca
     1621 cgtcaagtac gcgtacgaag atgtgctcga ctggatcgtg gaatcggtgt ctcctctagg
     1681 ggcagagtac caggcgcgcg tgcgccgcgc gttctcggag cgctggattg acgtgtacga
     1741 gaaccagggc aagagaagcg gcgcgtactc ggcgccggtg tacggcgcga atccgtacat
     1801 gttgatgaac tacaacgata cgctggacgc ggtgttcacg ctggcgcacg agctcggcca
     1861 ctcgatgcac acgatgctct cgcacgagac gcagccgttt gtctatgcgg gctacaccat
     1921 cttcgtcgcg gaagtgccgt cgacgctcaa cgaagcgttg ctgctcaact tcatgctgac
     1981 gcgcgacaag acgaaggaag agcgcatcgt cctcctgcag cacgcgatcg acgggatcgt
     2041 cggcacgttc tataaccagg tgctgttcgc cgatttcgag ctcgaggcgc atcgactggt
     2101 ggagcaggat cagccaatca cgtcagagac gttgagcggg atctacgcga agctgctcgg
     2161 tgaatattgg ggcgacgcgc tgtcagcgga tccgcgcgcg cagcacacct gggcgcggat
     2221 tccgcacttc ttccagtcgc cgtactacgt ctatcaatac gcgacctgct tcgcatcgac
     2281 tgccaagttg atggcggaga ttggcagcga ggacgcggcg atccgcaagg tcgcggtcga
     2341 gcgctatgtc aggttgctgc agtcgggcgg ttcggatcat ccgatgacgt tgttgcagcg
     2401 cgccggcgcc gatctcagga agccggagac cgtcaaagcc gtgtccgcgc agctcaacgg
     2461 cctcgtcgat cggctcgaac aggaacttgc ctaaaaggtc cgtctgaagg cggacaccac
     2521 aggatttcag gggaggcttc ggctcacctt acttgaaacc ctgtggtgtc cggctttagc
     2581 cggaccactg ctaatc
//
LOCUS       DATDEU010000637         4237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_565225, whole genome shotgun sequence.
ACCESSION   DATDEU010000637 DATDEU010000000
VERSION     DATDEU010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565225"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VIV63_15250"
     CDS             complement(<1..1038)
                     /locus_tag="VIV63_15250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6126008.1"
                     /translation="MAHSNNSSPGTADAARLLSKGSHFLRFDEPLETEFRTEHRTRLR
                     GWNRIAIIVSACTVVGFAILDHLVLSPEHSRVTNIVRFGMHVPCVILMLLLTSKRFYD
                     RWYDLGISFVAPLFGIGTVIMSAFSPPAEVPLVGARLLLASFFFYFMIGLRFREAARA
                     NALVFGALVIAAILGTMPTATATYLMFALFCANLIGCAGSIALEHANRSAFLEHRQLT
                     EVATHDGLTTLLNRAAFEDQIRRVWQQAQRDRQTIAVIMIDIDYFKAYNDRYGHIAGD
                     DCLRRVSSALRDAARRRPLDFVARYGGEELAAVLYGADKSYGESIARSLLTAVRDLRI
                     PHANSETQPYVT"
     gene            complement(1122..1310)
                     /locus_tag="VIV63_15255"
     CDS             complement(1122..1310)
                     /locus_tag="VIV63_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126009.1"
                     /translation="MKTKNQTEQHMLELAVATAANEDGISPASPRENATPVRKQAWSP
                     LEVWRTRVKTPTPRNPQI"
     gene            complement(1443..3023)
                     /gene="gshA"
                     /locus_tag="VIV63_15260"
     CDS             complement(1443..3023)
                     /gene="gshA"
                     /locus_tag="VIV63_15260"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY6126010.1"
                     /translation="MSAAIDRKFEQTLAALINSGEPEIIQGGRKGIERESLRVTPDGH
                     IAQTPHPRALGSALTNENITTDYSEALIELVSPTFRTSWELLQYLLDLHQFVYQHLGD
                     ELLWATSMPSVIDGESSIPIAQFGKSNVGRMKTVYRNGLGVRYGRMMQAISGVHYNYS
                     FPERMWDPWADIVQSRERGQPFISDRYFHLLRNYRRHGWIVLYLFGVSPVVCNSFLRG
                     RNVTLPRLSKDTSYEPYATSLRMSDLGYRNRNQAGLSVSVNSLDEYVRDLSRAISTPH
                     EPYEKLGVKVGDEYRQLNANILQIENEYYSFIRPKRVARSGERPTKALRRAGVEYVEV
                     RALDVSAFDPVGVNQNKMRFLEMFLALCLMKESQPIGLSEQDALDANHVTVARRGREP
                     GLTLQRDGRAVSMSAWARELIDSMQGIAEVFDRGDPARPFSAALAVQAAKVQNVALTP
                     SARMLAELESTGESFFELGLRMSRLHKEYFLALYPPNVERQAEFRAAADESLEAQKSV
                     EAADRMSFEQYLSEYSSG"
     gene            complement(3081..3716)
                     /locus_tag="VIV63_15265"
     CDS             complement(3081..3716)
                     /locus_tag="VIV63_15265"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY6126011.1"
                     /translation="MNCAPQWITAIAGLLLASAADVQAADFELTAAETQRVAKGEVVI
                     RTTLNPGARGGTVRAAMLIDAPPAVVFQTMSRCADAARYVPHLRMCRIREQAPDSSWA
                     IVEQEIDFGWYAPRLRYLSRAEFVTDRSISFRQIRGDFKANEGIWEFEPAANGDRTLL
                     RYRVYIDPPGYVPNWLARSTFKRELPDMLSALRRYCEAEQVLRAQANSSPK"
     gene            3830..4165
                     /locus_tag="VIV63_15270"
     CDS             3830..4165
                     /locus_tag="VIV63_15270"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEY6126012.1"
                     /translation="MKRVIAVLVLGLLASSPAFAFRCGTKLVSEGDSRAEVAHKCGEP
                     TDVVNQRSVFRRPVIWGYGRPRYVGEDYIEVPVETWLYNLGPNKLMRRIRFEGGVVVE
                     IETLGYGYN"
BASE COUNT          779 a         1260 c         1399 g          799 t
ORIGIN      
        1 ggtgacgtaa ggctgggttt cagaattggc gtgcggaatg cgcagatcgc gcaccgcggt
       61 cagcaggctg cgcgcaatgc tttcgccgta cgacttgtca gcgccataca gcacggccgc
      121 gagttcctcg ccgccataac gcgcgacgaa atcgagcggc cggcgccgcg cggcgtcgcg
      181 cagcgcgctc gaaacgcggc gcaggcaatc gtcgcccgcg atgtgtccgt agcggtcgtt
      241 gtaggccttg aagtagtcga tgtcgatcat gatgaccgcg atggtctggc ggtcgcgctg
      301 cgcctgctgc cagacgcggc ggatctgatc ttcgaacgcg gcgcggttca gcagcgtggt
      361 caggccgtcg tgggtcgcca cttcggtgag ctgacgatgc tcgaggaacg cggagcgatt
      421 cgcgtgctcg agcgcgatgg agcccgcaca gccgatgagg ttggcgcaga acaacgcgaa
      481 catgaggtag gtagctgtcg cggtgggcat ggtgcccagg atcgcggcga tgaccagtgc
      541 gccgaacacc agcgcattgg cgcgcgcggc ttcgcggaat cgcaggccga tcatgaaata
      601 gaagaagaac gacgccagca gcaggcgagc gcctaccagc ggcacttcgg cgggcggcga
      661 gaacgcggac atgatgaccg tgccgatgcc gaacagcggc gccacgaaac tgatgccgag
      721 gtcgtaccag cgatcgtaga aacgcttcga ggtgagcagc agcatgagga tgacgcaggg
      781 cacatgcatg ccgaagcgca cgatgttcgt gacgcgcgaa tgctccggcg acagaacgag
      841 gtgatcgaga atggcgaagc cgaccaccgt gcacgccgag acgatgatcg ctatgcggtt
      901 ccagccgcgc agccgggttc gatgttcggt gcggaattcg gtttcgagcg gttcgtcgaa
      961 acgcaggaaa tgcgatccct tggagagcag gcgcgccgcg tctgctgtgc ctggcgacga
     1021 attgttcgag tgcgccatta cgtccgtgcc tggcaggtcc tcagggaatg agtctgcttt
     1081 gtatgtgttc ggcagccgcg gcgcaagccg cgaggacggg tctagatctg cgggttacgc
     1141 ggcgtcgggg tcttcactcg agtacgccag acttccagcg ggctccatgc ctgcttgcgg
     1201 acgggcgtcg cgttttcacg cgggctcgcc ggcgaaattc catcttcgtt tgctgctgtc
     1261 gctaccgcca actcgagcat gtgctgctcg gtctgattct tggttttcat ttctgtccct
     1321 gaacaactgg tggaccgtta ggtctctctg gtcagggatt ctagtacgcc gattgttaaa
     1381 gaaaaggctt cgagaacaag attttatgga tatgtcaaga ccatgacgta cgtcacactt
     1441 tgtcagccgg aagagtactc cgataaatac tgttcaaaac tcattctatc ggcggcctcc
     1501 acggacttct gggcctccag cgactcgtct gccgcagccc tgaattcggc ctgccgctcc
     1561 acgttggggg gatacagcgc caggaaatac tctttatgaa gtcgggacat gcgcagaccc
     1621 agctcgaaaa acgactcgcc ggtggattcg agctcggcca gcatgcgggc cgacggcgtc
     1681 aacgccacgt tttgcacctt tgccgcctgc acggccagcg ccgccgagaa cggcctggcg
     1741 ggatcgccgc gatcgaacac ttcggcgatg ccctgcatgg agtcgatgag ctcgcgcgcc
     1801 catgcggaca tcgacaccgc gcgtccgtcc cgctgcaagg tgagacccgg ctcgcggcca
     1861 cggcgcgcca ccgtcacgtg attggcgtcg agcgcgtcct gttcggagag gccgatcggc
     1921 tgcgactcct tcatgaggca cagcgcgagg aacatctcga ggaaacgcat cttgttctgg
     1981 ttcacgccca ccggatcgaa ggcgctcacg tcgagcgcgc gcacttcgac gtattcgacg
     2041 cccgcgcgcc gcaatgcctt ggtcgggcgt tcgcccgaac gcgcgacacg cttcggccgg
     2101 atgaagctgt agtactcgtt ctcgatctgc aggatgttgg cgttcagctg ccggtattcg
     2161 tcgccgacct tcacgcctaa cttctcgtac ggttcgtgcg gcgtgctgat ggcgcgcgac
     2221 aggtcgcgca cgtattcatc gaggctgttg accgacacgg acaacccggc ctggttgcgg
     2281 ttgcgatagc ccaggtcgct catgcgaagg gacgtggcgt acggctcgta cgaagtatct
     2341 ttcgacaggc gcggcagggt cacgttgcgg ccgcgcagga aagaattaca aaccaccggc
     2401 gagacgccga acagatagag cactatccac ccgtggcgcc ggtaattgcg cagcaggtgg
     2461 aaatagcgat cggagatgaa cggctggccg cgctcgcgcg actggacgat gtccgcccag
     2521 gggtcccaca tgcgctcggg aaacgaatag ttgtaatgca cgccagagat ggcctgcatc
     2581 atccggccgt aacgcacgcc gaggccgttg cgatagacgg tcttcatgcg cccgacattc
     2641 gatttgccga actgcgcgat cggaatgctg gactcgccgt cgatgaccga cggcatggac
     2701 gtggcccaga gcaactcgtc gcccaggtgc tggtagacga actggtgcag atcgagcagg
     2761 tactgcagca gttcccagct cgtgcggaat gtcggcgaga cgagctcgat cagcgcttcc
     2821 gaataatcgg tggtgatgtt ttcgttggtg agcgccgaac ccagcgcgcg cggatgcggg
     2881 gtctgggcga tgtggccatc gggcgtcacg cgcagggatt cccgctcgat gcccttgcgg
     2941 ccgccctgaa tgatttcggg ctcgccgctg ttgatcagcg ccgcgagtgt ttgttcgaac
     3001 ttgcggtcga tggcggcgct catgggggcc ggcatcttgc cggtaccgcc tcatgatccg
     3061 caatcagtca atggagagag tcatttagga gagctgtttg cctgggcgcg caggacctgc
     3121 tcggcctcac aatatcggcg aagggcagac agcatgtccg gcagctcgcg tttgaaggta
     3181 gatcgggcaa gccagttcgg gacatatccc ggcgggtcga tgtacacgcg atatcgcagc
     3241 agcgtccggt cgccgttcgc ggcgggttcg aactcccaga tgccctcgtt ggccttgaag
     3301 tcgccgcgga tctgccggaa cgagatgctc cggtcggtga cgaattcggc gcgggacagg
     3361 taccggagtc gcggcgcata ccagccgaag tcgatttcct gttccacgat cgcccagctc
     3421 gaatcggggg cctgctcgcg gatccggcac atccgcaggt gtggcacgta ccgggcggcg
     3481 tccgcgcagc ggctcatcgt ctggaacacg acggccggcg gggcgtcgat gagcatggcc
     3541 gcccgaaccg tgccgccgcg cgcacccggg ttgagggtgg tacgaatcac gacttcgccc
     3601 ttggcgacgc gttgggtctc ggcggcagtc agctcgaaat ccgcggcctg cacatccgca
     3661 gcgctggcca gcaggaggcc cgcgattgcc gttatccatt gcggtgcgca attcatgttt
     3721 aacgaaatcc catgctcatg agtgccgccg ccagggtaat tggttcgtcg cttcgatcgt
     3781 ccagttccgt tcattgtcgg ttaagcagga tgggcctatt gtcggccaca tgaaacgcgt
     3841 aattgccgtg ctggtgctcg gcctgctggc ctcctcgccc gcgtttgcgt ttcgttgcgg
     3901 caccaagctc gtgagcgagg gcgactctcg cgccgaagtc gcacacaagt gtggcgaacc
     3961 cacggacgtc gtgaaccagc gaagcgtgtt tcgccgtccc gtgatctggg gctacggccg
     4021 gccgcgatac gtcggcgaag actatatcga agtgcccgtc gagacctggc tgtacaacct
     4081 cgggcctaac aagctcatgc gccgcattcg ctttgaaggc ggcgtggtgg tcgagatcga
     4141 gacgctgggg tacgggtata actaggaatg taggaatggg gtcaggcccc aattcctagc
     4201 aaaaagggtc agacctcgcg gagagggctg acccttt
//
LOCUS       DATDEU010000638         3146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1148497, whole genome shotgun sequence.
ACCESSION   DATDEU010000638 DATDEU010000000
VERSION     DATDEU010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148497"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VIV63_15275"
     CDS             complement(<1..609)
                     /locus_tag="VIV63_15275"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY6126013.1"
                     /translation="MNFSRREVLSMTGAAALASVAPQRLFAADAASGLESMTTGVVPI
                     GRAEFLARIDKARALMGKLGIGALLIEPGASLVYFTGVQWWRSERLTAAVIPREGDIA
                     IVTPHFEEPSVRESLRIPADVRVWNEDEDPLAAVAGILRDRKVTAPVAVEETVRFFAV
                     DALRRAMPGVHIVNGAPVVRGCRMVKSAAELALMQKASDITIA"
     gene            complement(648..1043)
                     /locus_tag="VIV63_15280"
     CDS             complement(648..1043)
                     /locus_tag="VIV63_15280"
                     /inference="COORDINATES: protein motif:HMM:NF039364.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HEY6126014.1"
                     /translation="MKDILVPVSPGEMLDKITILRIKAARMSDAAKVANVKHELALLE
                     KTWKDSGAAAVDLGSEEANLTRVNEALWVIEDDIRDEERAGRFGEKIIELARAVYITN
                     DERAAIKKRINILLGSSIVEEKSYKPYKP"
     gene            complement(1040..2113)
                     /locus_tag="VIV63_15285"
     CDS             complement(1040..2113)
                     /locus_tag="VIV63_15285"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY6126015.1"
                     /translation="MDAPEKVCLLRLSAIGDTCHVVPLLRTLQSAWPRTRFTWVIGKL
                     EAKLMSLIPDVELITVDKGAGLSAFTRLRTEMQRRGAFDLLLHLQLSLRASAAAACIP
                     APIKLGFDKPRARELQWLFTNRRIAERSREHVLDSFLGFADALGVPRQTLRWDIPLPP
                     AALAYAQQLIPTSTKGGQQTLVISACSSHRARNWMPERYAAVADHAVRKHDMRVILCG
                     GPAAIEREMAAAIAKHASVPLVDQVGKDTLPQLLALLARATVLLTPDSGPAHMATMVE
                     TPVIGLYAATNPARSGPYESRRWCVNAYDDAARKFLGKPADEIPWTRKIEVPGVMELI
                     EVPTVCSKLDELLALLAQMKGKP"
     gene            2126..2887
                     /locus_tag="VIV63_15290"
     CDS             2126..2887
                     /locus_tag="VIV63_15290"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HEY6126016.1"
                     /translation="MRRHWPVGAEVSEAAFEGGRMLYDTQRTSNFSADFFEPDYWRKH
                     DSIEGSALGRGTTYFIRTGDSSFVLRHYRRGGLMAKFSTDRYWWHGENETRAFAEWYL
                     TYHLHRAGLPVPTPIAARYQRAGLFYRADIITQRIENSEPLADRLLKGPLPLTQWIAV
                     GRCIRRFHDAGVCHADLNAHNILLTPAQVYVIDFDRGTLRKRGWWADTTLVRLYRSLE
                     KVTLLAAPESFTDEDWHSLLAGYRESAGLPQASMA"
     gene            2896..>3146
                     /locus_tag="VIV63_15295"
     CDS             2896..>3146
                     /locus_tag="VIV63_15295"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEY6126017.1"
                     /translation="MRFLYICIAYLISPFWIGALALRGFRDRIHWRGFSQRFGFGEKV
                     AARSIWVHAVSVGEVQAAVPLIEALLKRFSTIPLVLTTVT"
BASE COUNT          510 a          994 c         1050 g          592 t
ORIGIN      
        1 ggcaatggtg atgtcggatg ccttctgcat gagcgcgagt tctgctgccg acttcaccat
       61 gcgacagccg cgcaccacgg gcgcgccgtt cacgatgtga actccgggca tcgcgcgccg
      121 caaggcatcc acggcaaaga agcgcacggt ttcttccacg gcgaccggcg ccgtgacctt
      181 gcgatcgcgc aggatgcccg cgacggccgc gagcggatcc tcgtcctcgt tccagacgcg
      241 cacgtccgcc ggaatgcgca ggctttcgcg aaccgacggc tcctcgaaat gcggggtcac
      301 gatggcgatg tcgccctcgc gcggaatcac ggcggccgtg agccgttcgc tgcgccacca
      361 ctgcacgccg gtgaaataca ccagggaagc gccgggctcg atgagcagcg cgccgatgcc
      421 gagtttgccc atgagcgcgc gggccttgtc gatgcgagcg aggaactcgg cgcggccgat
      481 gggcacgacg ccggtggtca tggactcgag gccggacgcg gcatccgcgg cgaacaaccg
      541 ttgcggtgca accgatgcga gcgcggcggc gcccgtcatc gacaggactt cacgcctgct
      601 gaaattcatc cgacgcttct ccggggaagc cccacgcggc actttgctca aggcttgtag
      661 ggcttgtacg acttctcttc gacgatgctc gagcctaaca ggatgttgat gcgcttcttg
      721 atggcggcgc gttcgtcgtt ggtgatgtac acggcgcgcg cgagctcgat gattttttcg
      781 ccgaagcggc cggcgcgctc ttcgtcgcgg atgtcgtcct cgatgaccca cagggcttcg
      841 ttgacgcgcg tgagattggc ttcttcggac cccaggtcga cggcggcggc gccggagtct
      901 ttccaggttt tctcgagcag cgcgagctca tgcttcacgt tcgcgacttt cgcggcgtca
      961 ctcatgcgcg cggccttgat gcgcaggatg gtgatcttgt cgagcatttc gcccgggctc
     1021 acggggacga gaatatcttt catggcttgc ccttcatttg agcgagcaag gctagcagct
     1081 catcgagctt gctgcagacc gtcggcactt cgatgagctc catgacgccg ggcacctcta
     1141 tcttgcgcgt ccacggaatc tcgtcggccg gtttgccgag gaatttgcgt gccgcatcgt
     1201 cgtaggcatt gacacaccag cggcgcgact cgtacggccc gctgcgagcg ggattggtgg
     1261 ccgcgtacaa cccgatcacg ggtgtttcca ccatggtcgc catgtgcgcg gggccggagt
     1321 cgggagtcag caacacggtc gcgcgtgcga gcagcgccag cagctgcggc agcgtgtcct
     1381 tgccgacctg gtcgacgagc ggcaccgacg catgtttggc gattgcagcc gccatttcgc
     1441 gttcgatcgc ggcggggcct ccgcacagga tgacgcgcat gtcgtgtttg cgtaccgcgt
     1501 ggtcggccac ggcggcatag cgctcgggca tccagttgcg cgcgcgatgg ctcgagcagg
     1561 cgctgatcac cagcgtctgc tgcccgcctt tcgtcgaagt ggggatcagt tgctgggcat
     1621 aggcgagcgc cgcgggcggc agcggaatgt cccagcgcag cgtctggcgt ggcacgccga
     1681 gcgcgtcggc gaacccgagg aaactgtcca gcacgtgctc gcgggatctt tcggcaatgc
     1741 gccggttggt gaacagccac tgcagctcgc gggcgcgcgg cttgtcgaat cccagcttga
     1801 taggcgccgg tatgcaggcg gcggcggcgc tggcgcgaag cgagagttgc agatgcagca
     1861 gcaggtcgaa cgcgccgcga cgctgcatct cggtccgcag ccgcgtgaac gccgagagac
     1921 cggcgccctt gtccacggtg ataagctcca catccggaat gaggctcatc aatttcgctt
     1981 cgagtttgcc aatcacccag gtgaagcgcg tgcgcggcca ggcgctttgc agcgtgcgca
     2041 acagcgggac cacgtggcag gtatcgccga tggccgacaa tctcagcagg cagacttttt
     2101 ccggtgcatc catggaagtg agtgaatgag gcgccactgg ccggtgggcg cggaagtaag
     2161 cgaagccgcc ttcgagggcg gccggatgct atacgacacc caacgcacga gcaatttctc
     2221 ggcggacttc ttcgagccgg attactggcg caagcacgat tccatcgaag gcagcgccct
     2281 tggtcgtgga accacgtact tcatccgcac gggcgacagc agtttcgtgc tgcggcacta
     2341 tcgccgcggc gggctgatgg caaaattttc cacggatcgc tactggtggc atggcgagaa
     2401 cgaaacccgc gcattcgcgg agtggtatct cacctatcac ctgcatcgcg cgggcctgcc
     2461 ggtgccgacg ccgatcgcgg cgcgttatca gcgcgcgggc ctgttctatc gcgccgacat
     2521 catcacgcag cgaatcgaga acagtgagcc gctcgcggac cggctgctca aggggccgct
     2581 gccgctcacc cagtggatcg ctgtgggccg ttgcatccgg cgctttcacg atgccggcgt
     2641 gtgtcacgcg gatttgaacg cgcacaacat cctgctgacg ccggcgcagg tctacgtcat
     2701 cgatttcgat cgcggcacgc tgcgcaagcg cggctggtgg gcggatacga cgctggttcg
     2761 gctctatcgt tcgctcgaga aggtcacgct gctcgcggcg cccgagagtt tcaccgacga
     2821 agactggcat tcgctgctgg ccggatatcg tgaatccgcg ggtctgccgc aggcttccat
     2881 ggcctgacgg tcaacgtgcg tttcctatac atctgcattg cctacctgat ctcgccgttc
     2941 tggatcggcg cgctggcgct gcgcggcttt cgcgatcgca tccactggcg ggggttttcg
     3001 cagcgcttcg ggttcgggga aaaggttgcc gcgcgcagca tctgggtgca tgcggtgtcg
     3061 gtgggcgaag tgcaggccgc ggtgccgctc atcgaagctc tgctcaagcg tttttccacg
     3121 atcccgctgg tgctcaccac cgtcac
//
LOCUS       DATDEU010000639         2203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1439533, whole genome shotgun sequence.
ACCESSION   DATDEU010000639 DATDEU010000000
VERSION     DATDEU010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439533"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2203)
                     /locus_tag="VIV63_15300"
     CDS             complement(<1..>2203)
                     /locus_tag="VIV63_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY6126018.1"
                     /translation="NSSLGETAKASEPFVRQVFIGRGPDTADEADFERKLYVIRKRAY
                     NEIRVSTIGGAEFWYIVSLSHKTLVYKGMLTTNQLDQYFLDLQNPLMESAIALVHSRF
                     STNTFPSWDRAHPYRCIAHNGEINTLRGNINWMRAREALFQSPLFGEDIRKIPPIVNP
                     NGSDSSMFDNVLELMVLSGRSLPHAVRMMIPEPWSKHASMDSGRRAFYQYHSSLMEPW
                     DGPASICFTDGKSIGAVLDRNGLRPGRYYVTKDDLVIMASEAGVLEFPPENIVRKGRL
                     QPGRMFLVDTEQGRIVEDEEIKAQIINERPYAEWLKQHLVHLNDLPAAPELPLPEPVA
                     LAKRQVSFGYTFEDQRILMAPMARDGNEGVGSMGNDTPLAALSQKSRMLYDYFKQLFA
                     QVTNPPIDCIREEIITSSDVWLGSEGNLLDPRPGDCRRLELPGPVLTNEEFAKVRRMS
                     LPGLKVGTLRILFRAERGEDGLIEAIEEMRAEARRMIEEDEVNILILSDRGVNKEFAA
                     IPSLLAVSGLHHYLIRHGLRMKVSLALETGDAREVHHFALLIGFGCSVINPYLAFETL
                     DGMIRDEMLPGVEHKLACKNFAKAAVKGVVKVMSKMGISAVQSYHGAQVFEAVGLRQD
                     VIDEYFSGTASRVGGVGLDVIAREVLMRHTAAFAERKPQSQTELPSGGQYQWRQDGEF
                     HLFNPESIHRLQKSVRTGSYATYKSYAELIDDRAKNLSTLRGLLDFKQGESI"
BASE COUNT          374 a          681 c          730 g          418 t
ORIGIN      
        1 aatggattcg ccctgcttga agtcgagcag gccacgcagc gtggagaggt tcttcgcgcg
       61 atcgtcgatg agttcggcgt aactcttgta tgtggcgtac gaacccgtcc gcaccgactt
      121 ctgcaggcgg tggatcgatt ccggattgaa cagatggaac tcgccatcct ggcgccactg
      181 gtactggcca ccggacggta gttcggtctg gctctgcggc ttgcgttcgg cgaacgcggc
      241 cgtgtggcgc atgagcactt cgcgcgcgat cacgtcgagg cccacgccgc cgacgcgcga
      301 cgccgtgccg gaaaagtatt cgtcgatgac gtcctggcgc agacccacgg cttcgaacac
      361 ctgcgcaccg tggtaactct gcacggccga gatgcccatc ttggacatga ccttcacgac
      421 gcccttgacc gcggctttcg cgaaattctt gcacgcgagc ttgtgctcga cgcctggcag
      481 catttcgtcg cggatcatgc cgtccagcgt ttcgaacgcg aggtacgggt tgatgaccga
      541 gcagccgaag ccgatgagca gcgcgaagtg atgcacctcg cgcgcgtcgc cggtctccag
      601 cgccagcgac accttcatgc gcaggccgtg gcggatcagg tagtgatgca ggccggagac
      661 ggccagcaac gacgggatcg ccgcgaattc cttgttcacg ccgcggtcgg aaagaatgag
      721 gatgttgacc tcgtcctctt cgatcatgcg gcgcgcttcg gcgcgcatct cttcgatggc
      781 ctcgatgagg ccgtcttcac cgcgctcggc gcggaacagg atgcgcaacg tgccgacctt
      841 gaggccgggc agcgacatgc gccgcacctt ggcgaactcc tcgttggtca gcacgggtcc
      901 cggcagctcg agccggcggc agtcgcccgg acgcgggtct aacaagttgc cttccgagcc
      961 cagccacaca tccgacgagg tgatgatttc ttcgcggatg caatcgatgg gcgggttggt
     1021 gacctgcgcg aacagctgct tgaagtagtc gtacaacatg cgcgacttct gcgagagcgc
     1081 ggccagcggc gtgtcgttgc ccatggagcc gacgccttcg tttccgtcgc gcgccatcgg
     1141 cgccatgagg atgcgctggt cttcgaacgt gtaaccgaaa gacacctggc gcttggcgag
     1201 ggccacgggt tccggcagcg gcagctcggg cgccgcgggc agatcattga ggtgaacgag
     1261 gtgctgcttc agccactcgg cgtacggccg ctcgttgatg atctgcgcct tgatctcttc
     1321 gtcttcgacg atgcggccct gctcggtgtc cacgaggaac atgcggcccg gctgcaggcg
     1381 gcccttgcgc acgatgttct cgggggggaa ctcgagcaca ccagcctcgc tcgccatgat
     1441 gacgaggtcg tccttggtga cgtagtagcg gcccgggcga aggccgttgc gatcgagcac
     1501 ggcgccgatg cttttcccat cggtgaagca gatgctggct ggcccgtccc acggctccat
     1561 caaactactg tggtactggt agaacgcgcg gcggcctgag tccatcgagg cgtgcttcga
     1621 ccacggctcg gggatcatca tcctgacggc gtgcggcagc gaacggccgg agagcaccat
     1681 gagctcgagc acgttgtcga acatcgagga gtccgaaccg ttcgggttca cgatcggcgg
     1741 gatcttccgg atgtcttcgc cgaacaatgg cgactggaac agcgcctcgc gcgcgcgcat
     1801 ccagttgatg ttgccgcgaa gcgtgttgat ttcgccgttg tgcgcgatgc agcggtacgg
     1861 atgcgcgcgg tcccagctcg ggaaggtgtt cgtgctgaag cgcgagtgca cgagagcgat
     1921 tgcgctctcc atgagcgggt tctgcaggtc gaggaagtac tggtcgagct gattcgtggt
     1981 cagcatgccc ttgtacacaa gcgtcttgtg cgacagcgac acgatgtacc agaactccgc
     2041 gccgccgatc gtcgacacgc ggatctcgtt gtacgcgcgc ttgcggatca cgtagagctt
     2101 gcgctcgaag tccgcctcgt ccgcggtgtc cggaccacgg ccgatgaaca cctggcgcac
     2161 gaacggctcg gacgccttgg cggtttcgcc gagcgacgag ttg
//
LOCUS       DATDEU010000640         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1876032, whole genome shotgun sequence.
ACCESSION   DATDEU010000640 DATDEU010000000
VERSION     DATDEU010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876032"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VIV63_15305"
     CDS             complement(<1..675)
                     /locus_tag="VIV63_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY6126019.1"
                     /translation="MDREQGIKLMQDLLRRVVDKKASDLFITAGFPPAIKIDGEVRPQ
                     SERALTGEQSAVLVRSIMNDRQTKEFDATKECNFAIAPPGIGRFRVNTFVQQGNTGCV
                     IRLINSKIPTLEELELPPVLKEVVLSKRGLVIMVGGTGSGKSTSLAAMVGYRNEKTRG
                     HIVTIEDPVEYVHQHKGCVITHREVGVDTDNWHTALKNTLRQAPDVILIGEIRDRETM
                     EYGIQFA"
     gene            complement(675..1715)
                     /locus_tag="VIV63_15310"
     CDS             complement(675..1715)
                     /locus_tag="VIV63_15310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY6126020.1"
                     /translation="MAVDVAQLLAFAVKNNASDLHLSAGVPPMIRVDGDMKRINMPAL
                     THKEVHGMVYDIMNDKQRKAYEEFFETDFSFEIPKLARFRVNAFNQNRGAGAVFRNIP
                     SVILSLDDLAAPKIFRDLCMLPRGLVLVTGPTGSGKSTTLAGMVNHCNDNRPDHIITI
                     EDPIEFVHESKRCLMNQREVHRDTLGFSEALRSALREDPDVVLVGEMRDLETIRLALT
                     AAETGHLVFGTLHTSSAAKTIDRVVDVFPAQEKEMVRAMLSESLRAVISQTLLKRNGG
                     GRIAAHEIMIGTPAIRNLIREGKIAQMFSAIQTGGAVGMQTLDQCLTDLVAKGVVSKE
                     EARYKATNKDAL"
     gene            1770..2486
                     /locus_tag="VIV63_15315"
     CDS             1770..2486
                     /locus_tag="VIV63_15315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY6126021.1"
                     /translation="MLSKPQNCSESVQIAQSRIAGACLAASRSVDSVTLLAVSKGQSS
                     AAIDCAARAGVEHFGENFLQESLPKMESLAGRELTWHFIGRLQANKTRAVAENFAWVH
                     AVDRLKIAERLSAQRPSQLPPLNLCLQLHVGGEQSKGGVEAAEIAPLAAAIRELPRIR
                     LRGLMCMPPAETDVDRQRQWFRETRQVFDYLNEHGMSLDTLSMGTSMDFESAIVEGAT
                     MVRIGTAIFGPRDAVNIPAT"
BASE COUNT          473 a          791 c          843 g          519 t
ORIGIN      
        1 ggcgaactgg atgccgtact ccatggtctc gcgatcgcgg atttcgccga tcaggatgac
       61 gtcaggggcc tggcgcagcg tgttcttcaa cgcggtatgc cagttgtcgg tatccacgcc
      121 cacttcacga tgcgtgatca cgcagccctt gtgctggtgc acgtactcga ccggatcttc
      181 gatcgtgacg atgtggccgc gtgtcttttc gttgcggtag ccgaccatcg cggcgagcga
      241 agtcgactta cccgaacccg tgccgcccac catgatcacg aggccacgct tggaaagcac
      301 gacctctttg agtacgggtg gcaactcgag ttcttcgagt gtcggaatct tggaattgat
      361 gaggcggatg acgcagccgg tgttgccctg ctgcacgaag gtgttcacgc ggaagcggcc
      421 gatgcccggc ggcgcgatcg cgaaattaca ttccttggtg gcgtcgaatt ccttggtctg
      481 ccggtcgttc atgatggaac gcaccagcac cgcggattgc tcgcccgtca gcgcgcgctc
      541 ggactgcggc cgcacttcgc cgtcgatctt gatggcgggc ggaaaacccg ccgtgatgaa
      601 caggtcggag gccttcttgt cgacgacgcg acgaagaaga tcctgcatca gttttatgcc
      661 ttgctcgcga tccattacag cgcatcctta ttcgtggcct tgtaacgggc ctcttccttg
      721 ctgacgacgc ccttggcgac gagatccgtc aggcactggt cgagtgtctg catgcccacc
      781 gcgccgcccg tctggatggc cgagaacatc tgcgcaatct tgccttcgcg aatcaggtta
      841 cggatcgcgg gggtgccaat catgatttca tgcgcggcga tacggccgcc gccgttgcgc
      901 ttgagcagcg tctgcgagat gacggcgcgc agcgattcgg agagcatcgc gcggaccatt
      961 tccttttcct gcgccgggaa cacgtcgacc acgcggtcga tggtcttggc ggcagagctc
     1021 gtatgcagtg tgccgaacac gaggtgtccc gtttcggcgg cagtgagcgc gaggcggatc
     1081 gtttcgaggt cgcgcatttc gccgaccagg acgacgtccg ggtcttcgcg caatgcggac
     1141 ctcaatgcct cggagaagcc gagagtatcc cggtgtactt cgcgctggtt catgaggcag
     1201 cgtttgctct catgcacgaa ttcgatgggg tcctcgatcg tgatgatgtg atcgggacgg
     1261 ttgtcattac agtggttcac catgcccgcg agcgtggtgg acttgcccga gcccgtcggg
     1321 ccggtgacca gcacgaggcc gcgcggcagc atgcacaggt cgcggaagat tttcggcgcg
     1381 gcgagatcgt ccagcgacag gatgaccgag ggaatgttgc ggaacacggc gccggcgccg
     1441 cggttctggt tgaacgcgtt gacgcggaag cgcgcgagct tcgggatctc gaatgaaaag
     1501 tcggtctcga agaattcttc gtaagccttg cgctgcttgt cgttcatgat gtcgtacacc
     1561 atgccgtgca cttccttgtg ggtaagcgcg ggcatgttga tacgtttcat gtcgccgtcg
     1621 acacgaatca tgggtggcac accagcggac aggtgcaagt ccgaggcgtt attctttacg
     1681 gcgaaagcga ggagctgggc tacgtcgacg gccatgcggt gtcccttgaa aagcgccgtt
     1741 gagtatagac ccgggtatca ctgatcaata tgttatccaa gccgcagaat tgctctgaga
     1801 gcgtccagat cgcacagagt cgtatcgcag gggcctgcct ggcagcctcc cgaagtgtcg
     1861 actcggtcac gcttctggcc gtttcgaagg gccagagcag cgcagcaata gactgcgccg
     1921 cgcgagccgg cgtcgagcat ttcggcgaga atttcctgca ggaaagcctg cccaagatgg
     1981 agtcgctggc cgggcgtgag ctcacctggc atttcatcgg gcggctgcag gctaacaaga
     2041 cgcgcgccgt ggccgagaat ttcgcctggg tacacgcggt cgaccgcctc aagatcgccg
     2101 agcgcctttc ggcgcagcgc ccgtcgcagt tgccgccact caacctctgc ctgcagctcc
     2161 acgtgggtgg cgagcagagc aagggtggcg tcgaagccgc ggagatcgca ccgctcgcgg
     2221 ccgcgatacg cgaactaccg cggatcaggc tccgggggct catgtgcatg ccgcccgccg
     2281 aaaccgacgt ggatcgccag cggcagtggt ttcgcgagac gcgccaggtg ttcgactatc
     2341 tcaacgaaca cggaatgagt ctcgacacct tgtccatggg caccagcatg gatttcgaat
     2401 ccgccatcgt cgaaggcgcc accatggtcc gcatcggcac ggccatcttc gggccacgcg
     2461 acgcagttaa tatcccggcc acatgaacat caatgcagca ttcgtcggcg gcggcaacat
     2521 gggtggcgcg ctgattcgcg gcctcatcgc ccgcggcctt gccgcgcaga acatccgcgt
     2581 gggtgaagcg cacgcgccgc gacgcatcgc gctcgcggac gaactc
//
LOCUS       DATDEU010000641         3215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2167987, whole genome shotgun sequence.
ACCESSION   DATDEU010000641 DATDEU010000000
VERSION     DATDEU010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167987"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /locus_tag="VIV63_15320"
     CDS             <1..1708
                     /locus_tag="VIV63_15320"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEY6126022.1"
                     /translation="TRVFPQVVLELADKYGDSPALLSDTECFTYRELAARSNRYARWA
                     LAQGVRKGDTVCLIMPGRPEFLAIWVGITRVGGVVALVNTHLTGTALAYCINAVSPKH
                     IIVAAELFGSLESARAHITGDAQIWLHGNADSNFARIDREIDGLSGADLPASDLPALT
                     IEDGALYIYTSGTTGLPKAANMNHYRVMLASNAFAGVMDTRASDRMYDCLPLYHTAGG
                     VVATGALLVRGGSVVIRDKFSASEFWDDIVRWECTCFQYIGELCRYLINSPPSPNERA
                     HHLRLACGNGLRPDVWPEFKRRFRIPRIIEFYAATEGNVSLFNFDGKEGAIGRLPWWV
                     AGRFPTKIVRFDVERQQPVRNEQGFCIECDADEPGEVIGKILKDPSKPGQRFEGYASK
                     AESDRKILRDVFEKGDIWFRTGDLMRKDKNGYFYFIDRIGDTFRWKGENVSTTEVEEA
                     IGRFDDVMEANVYGVEVPGRDGRAGMAAIVGKDNLNLAGLRDHLARHLPEYARPMFLR
                     LREANDVTSTFKSKKIDLVKQGFDPSRTDDPIYFNDPRSKAFVRLDPALYEDITAGRV
                     RL"
     gene            1705..2277
                     /locus_tag="VIV63_15325"
     CDS             1705..2277
                     /locus_tag="VIV63_15325"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY6126023.1"
                     /translation="MTGDAITPATATIAPVDVLAFWRTAGPDKWFAKDDAFDAEIAAR
                     FGTLWCAAANDELASWETTPEGALALVLVLDQFPRNMFRGQARTYETDAVARAVADRA
                     LKRGFDRDVPQLERRFFYLPFMHSEILADHERCLELARGLGDDEVTQYAELHADIIRR
                     FGRFPHRNAMLGRVMTPDEQAFLDAGGFAG"
     gene            2355..>3215
                     /locus_tag="VIV63_15330"
     CDS             2355..>3215
                     /locus_tag="VIV63_15330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6126024.1"
                     /translation="MRQSWAYDEEGGEFSPPSGINERLTCDLLIAGGGLSGLSAAEAA
                     LRRGLDVIVIEKGAFGKDAASGLNAGQFLTGWAKPVDVMLAELAQQELRGGLAFDQAQ
                     LHAQRRVRAFLRRTVEGCQRLAELDHHYNLRASVQHGAVMAAVTEVDMAGLRAAYQFM
                     DDSNLRALMPMTDKRRARFFETLSAAQVQRRYGTAEGFYAGGVVDRFGGSFRPRKFLI
                     GLARALQKRGVRFFQGTEAQALDFSDGQVSVFCGNGANIRANTLFMANAYARHINGDV
                     LERAIFEYDYV"
BASE COUNT          575 a         1042 c         1021 g          577 t
ORIGIN      
        1 gacgcgcgtg tttccgcagg tcgtcctgga actcgcggac aaatacggcg actcgcccgc
       61 actgctctcc gacacggagt gcttcaccta ccgcgagctt gccgcgcgct ccaaccgcta
      121 cgcgcgctgg gcgttggcgc aaggtgtgcg caagggcgat acggtctgcc tgatcatgcc
      181 gggtcgaccc gaattcctcg cgatctgggt cggcatcacg cgcgtcggcg gcgtggtcgc
      241 gctcgtcaac acccatctga ccggaacagc gctggcctat tgcatcaacg ccgtaagtcc
      301 gaagcacatc atcgtggccg cggaattgtt cggatcactg gagagcgcgc gtgcacacat
      361 caccggcgac gcgcagatct ggctgcacgg caatgccgac tccaatttcg cgcgcatcga
      421 tcgcgagatc gacggtcttt ccggcgccga cctcccggcg tccgatcttc ctgccctgac
      481 catcgaggac ggcgcgcttt acatctacac ctcgggcacg accggcctgc cgaaagcggc
      541 caacatgaac cattaccgcg tgatgctcgc gagcaatgcc ttcgccggcg tcatggatac
      601 gcgcgccagc gaccgcatgt acgattgtct gccgctttat cacaccgccg gcggcgtggt
      661 cgcgaccggc gcgcttcttg tccgcggcgg ctcggtggtg atccgggaca agttttccgc
      721 aagcgagttc tgggacgaca tcgtgcgctg ggagtgcacc tgctttcaat atatcggcga
      781 gctctgccgc tacctcatca actcgccgcc gagccccaac gagcgcgcgc atcatcttcg
      841 cctcgcctgc ggcaacggcc tgcggcccga cgtctggccg gaattcaagc ggcgtttccg
      901 cattccgcgc atcatcgagt tctacgccgc caccgaaggc aacgtttcgt tgttcaattt
      961 cgatggcaag gaaggcgcca taggccggct tccgtggtgg gttgccggcc gctttccgac
     1021 caagatcgtg cgcttcgacg tggagcgcca gcagccggtc cgcaacgaac agggcttctg
     1081 catcgaatgc gacgccgacg agccggggga ggtcatcggc aagatcctga aagatccgtc
     1141 gaagcccggc caacggttcg aaggctatgc gagcaaagcc gaaagcgacc gcaaaatcct
     1201 gcgtgacgtg ttcgagaagg gtgacatctg gttccgcacc ggcgacctga tgcgcaagga
     1261 caagaacggc tacttctatt tcatcgaccg catcggcgac accttccgct ggaagggcga
     1321 gaacgtctcg accaccgagg tcgaggaggc gatcggacga ttcgacgatg tgatggaggc
     1381 caatgtctac ggagtcgaag tgccgggacg cgacggccgg gccggcatgg ccgcgatcgt
     1441 cggcaaggac aacctcaacc tagccggcct tcgcgaccac ctggcgcgac acctgccgga
     1501 atatgcccgg cccatgttcc tgcgtttgcg tgaagccaac gacgtcacct cgaccttcaa
     1561 gtcgaagaaa atcgaccttg tgaagcaagg tttcgatccg agccggaccg acgatccgat
     1621 ctatttcaat gatccccgca gcaaggcctt cgtccggctt gaccccgcgc tgtacgaaga
     1681 catcacagcg gggcgtgtgc ggctgtgacc ggcgatgcca tcacccctgc gaccgcgacg
     1741 atcgcgccgg tggacgtgct cgcgttctgg cgcaccgccg gcccggacaa atggtttgcc
     1801 aaggacgacg ctttcgacgc cgagatcgcg gcgcgctttg gcacgctctg gtgcgcggcc
     1861 gcaaatgacg agcttgcatc gtgggagacg acgcccgagg gcgcgctcgc gctcgtcctc
     1921 gttctcgacc agtttccgcg caacatgttc cgcggacagg cgcggaccta cgagaccgat
     1981 gcggtcgccc gcgccgtcgc cgatcgcgct ctcaagcgcg gattcgaccg cgatgtgccg
     2041 cagctggagc gccgattctt ctatctgccg ttcatgcatt ctgaaattct agccgatcac
     2101 gaacgatgcc tcgagctggc gcgtggtctg ggcgacgatg aagtcacaca atacgccgag
     2161 ctccacgccg acatcattcg ccgcttcggc cggtttcccc atcgcaacgc gatgctggga
     2221 cgggttatga caccggacga gcaggccttt ctcgacgccg gcggcttcgc cggctgagac
     2281 gcattgcgaa ttcgtatgac ggatcgcaaa cgatccgcat tgaatgcgca tcgcgccgca
     2341 ttacgtttgg agacatgcgg cagagctggg cttacgacga ggaaggcggg gagttttcgc
     2401 ctccgtccgg catcaatgaa cgcctgacct gcgatctcct gatcgccggc ggtggcctat
     2461 cgggtctgag cgcggcggaa gcagcgctgc gccgcggcct cgacgtcatc gtgatcgaga
     2521 agggcgcctt cgggaaggat gcggcgtccg ggctcaatgc gggacaattc ctgactggat
     2581 gggcaaagcc ggtggacgtc atgttggcgg aactggccca acaggagctg cggggtggcc
     2641 tcgccttcga tcaggcgcag ttgcacgccc aacgccgggt gagggcattc ctgcgccgca
     2701 cggtcgaggg atgccagcgt ctcgccgaac tcgaccatca ctacaatctg cgcgcgtcgg
     2761 tgcagcacgg cgcggtcatg gcggcggtga ccgaggtgga tatggcgggc ctgcgcgccg
     2821 cctaccagtt catggacgac agcaatctgc gcgcgctcat gcccatgaca gacaagcgcc
     2881 gggcgcgatt cttcgaaacc ctctccgccg cacaggttca gcggcggtac ggcaccgcgg
     2941 agggttttta cgccggcggc gtggtcgacc gcttcggcgg cagttttcgt ccgcgcaagt
     3001 ttctcatcgg actggcgcgc gcgctgcaga agcgcggggt gcgcttcttt cagggcacgg
     3061 aggcgcaggc gctcgatttt tccgacggcc aggtcagcgt gttctgcggc aacggggcga
     3121 acatccgcgc caatacgctg ttcatggcga atgcctatgc gcggcacatc aacggcgacg
     3181 tgctcgagcg tgcgatcttc gagtacgatt acgtc
//
LOCUS       DATDEU010000642         3174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_566229, whole genome shotgun sequence.
ACCESSION   DATDEU010000642 DATDEU010000000
VERSION     DATDEU010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566229"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VIV63_15335"
     CDS             complement(<1..648)
                     /locus_tag="VIV63_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126025.1"
                     /translation="MQRPLWIVVLLLAGLAHAGDVATPAKSPLRVLLLFPSDLLMPWA
                     VAQGENTRRAILGAVPDRVEFFAEGLDGFRFPGSHNEDEFVSLLIKRYSAVPPDLIIV
                     HGPMEEFIERQRGKLWPKTPVMAVGSLAEPFSKSRYPREVPGTSVSFDSAATVELALL
                     LQPDAKRVVVVGGSSQYSRAEIQQATDQLEIFRGRLEVLYLVDLPVDEMKRRLAAL"
     gene            complement(674..2017)
                     /gene="pmbA"
                     /locus_tag="VIV63_15340"
     CDS             complement(674..2017)
                     /gene="pmbA"
                     /locus_tag="VIV63_15340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEY6126026.1"
                     /translation="MQEVDGTRIPELKSIVSESLQLARNLGAAQAEADVSLQKGLTTT
                     VRLGEVETVEYQRDRGMGITVYFGKRKGSASTADLSPRAVAETVEKACDIARYTAEDE
                     CSGLADPEELARDIPDLDLDHPWDLPPEQAVETARECEAAGRKVDSRITNSEGASVSS
                     HRGVRVYGNSHGFLEGFPSTSHSVSCVLLAQTGDDMQRDYWYASSRDARELESSESIG
                     RKAGERAVARLGARKISTQKARVLYAPEVARGLIGHFLGAVRGSSQYRKSSFLLDASG
                     KQIFPSFVELRERPHIMKALGSSPFDGEGVATRNRDLVSDGVLQGYILGSYSARKLGL
                     KTTGNAGGTHNVLVETKNGGMEIGALMRELGTGLLVTELMGQGVNGVTGDYSRGASGY
                     WVENGVQAYPVHEITIAGNLKDMYNNIAAIGSDVDVRGGVRVGSILISEMTIAGE"
     gene            2033..2554
                     /gene="yjgA"
                     /locus_tag="VIV63_15345"
     CDS             2033..2554
                     /gene="yjgA"
                     /locus_tag="VIV63_15345"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY6126027.1"
                     /translation="MRGKDENEEYRGPSKSMLKRDAAAAQDLGTRLIELKEAELLALD
                     LPETLYDAIVLAKRITSRGGLARQRQYIGKLMRDIDHAPIEALLNAESRATAQDAEKH
                     KRIEAWRLRLLTEGKAALDDLLEWRPDADRKALQALITKATSERVDSGSREGASRELF
                     RSIRSLFDDSISR"
     gene            2551..3057
                     /gene="purE"
                     /locus_tag="VIV63_15350"
     CDS             2551..3057
                     /gene="purE"
                     /locus_tag="VIV63_15350"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY6126028.1"
                     /translation="MKPLVGIIMGSTSDWETMRAAAEALDKLGVPYETRVVSAHRTPD
                     LLFEYASTAQTRGIEVIVAGAGGAAHLPGMVASKTTLPVLGVPVQSKALNGLDSLLSI
                     VQMPAGIPVATFAIGVAGATNAGLAAAAILANKRPEIDAALKKFRAEQTAKVLAHSDP
                     RMADPSKL"
BASE COUNT          567 a         1070 c          990 g          547 t
ORIGIN      
        1 caacgccgcg agccggcgtt tcatctcgtc cacgggcaga tcgaccagat aaaggacttc
       61 caggcgtccg cgaaagatct cgagctgatc ggtggcctgc tggatctcgg cgcggctgta
      121 ttgcgaactg ccacccacga ccacgacgcg cttcgcatcc ggctgcaata gcagggcgag
      181 ctccacggtg gcggccgaat cgaagctcac gctcgtgcct ggaacttcgc gagggtaacg
      241 acttttcgag aatggctcgg cgaggctgcc aaccgccatc acgggagtct tcggccacaa
      301 cttgccgcgc tgccgctcga tgaactcctc catgggcccg tgaacgatga tgaggtcggg
      361 cggcacggcc gagtaacgct tgatcagcag cgagacgaat tcgtcctcgt tgtgcgatcc
      421 gggaaaccgg aatccatcga ggccctcggc aaaaaattcc acgcggtccg gcaccgcgcc
      481 aagaatcgcg cgccgggtat tctcgccctg cgccaccgcc cagggcatca gaagatcgct
      541 cggaaacagc agcaacacgc gcagcggcga tttcgcgggc gtagcgacgt cgccggcatg
      601 cgccaggccc gcgagcagga gaaccacgat ccacagcggt cgttgcattc gatgttcgga
      661 ccggtcgacg aggctattcg ccagcgatgg tcatctcgct gatgagaatc gagccgaccc
      721 gcactccccc gcgtacgtcg acgtcactgc cgatcgccgc gatgttgttg tacatgtcct
      781 tgagattgcc cgcgatggtg atctcgtgca ccggataggc ctgcacgccg ttctccaccc
      841 aataacccga agcgccgcgt gaatagtcgc cggtcacgcc gttgacgccc tgccccatga
      901 gctcggtgac taacaacccg gtgccgagct cgcgcatgag cgcaccgatc tccatgccac
      961 cgttcttcgt ttccaccagc acgttgtgcg taccaccggc gttgccggtg gtcttgagtc
     1021 ccagcttgcg ggccgagtag ctgccgagga tgtagccctg caacacgccg tcgctcacca
     1081 ggtcccgatt gcgcgtggcc acaccctcgc catcgaacgg gctgctgccg agcgccttca
     1141 tgatgtgcgg ccgttcgcgc agttccacga acgacggaaa aatctgcttg ccggaagcat
     1201 cgagcaggaa cgagctcttg cggtactggc tgctgccacg cacggcgccg agaaaatgcc
     1261 cgatgagtcc tcgcgccact tcgggcgcgt acagcacccg cgccttctgc gtgctgatct
     1321 ttcgcgcgcc gagccgcgcc accgcgcgct cgcccgcctt gcggccaatg ctctcggagc
     1381 tttcgagctc gcgcgcgtcg cgcgacgagg cgtaccagta gtcgcgctgc atgtcgtcgc
     1441 cggtctgggc gagcagcaca caactcacgc tgtgactggt cgacgggaaa ccttcgagga
     1501 agccatgcga attgccgtac acgcgcacac cgcggtgact gctgacgctg gcgccttccg
     1561 aattggtgat gcgcgagtcg accttgcgac cggccgcttc gcattcccgc gcggtctcca
     1621 cggcctgctc gggcggcagg tcccaggggt gatcgagatc gagatcggga atgtcacgcg
     1681 ccagctcttc gggatccgcg agaccggaac actcgtcttc ggcggtatac cgcgcgatgt
     1741 cgcaggcctt ctcgacggtt tcggccacgg cccgcggcga caggtcggcg gtgctcgccg
     1801 agcccttgcg cttgccgaag tagacggtga tgcccatgcc gcgatcgcgc tggtattcga
     1861 cggtttcgac ttcgccgagg cgcaccgtgg tcgtcaggcc tttctgcagg ctgacatcgg
     1921 cttcggcctg ggcagccccc aggttgcggg caagctgcaa cgattcggaa acgatgctct
     1981 tgagctcggg gattcgggta ccgtctactt cctgcatggc tagaattgta cgatgcgcgg
     2041 caaagacgaa aacgaggaat atcggggtcc cagcaagagc atgctcaagc gtgatgccgc
     2101 ggctgcccag gatctgggta cccgcctcat cgagctgaag gaagccgagc tcctcgcgct
     2161 cgacctgccc gagaccctgt acgacgccat cgtgctcgcg aagcgcatca ccagccgcgg
     2221 cgggctcgca cgccagcggc agtacatcgg caagctcatg cgcgatatcg atcacgcccc
     2281 catcgaggcg ctgctcaacg cggaatcgcg cgccacggcg caggacgccg agaaacacaa
     2341 gcgcatcgag gcatggcgcc tgcggctgct caccgaaggg aaggcggcgc tcgatgacct
     2401 gctcgagtgg cgcccggacg cggatcgcaa ggccctgcag gcgctcatca ccaaggccac
     2461 gagcgagcgc gtcgactcgg gcagccggga gggtgcgtcg cgcgagctgt tccgttcgat
     2521 tcgttccttg tttgacgata gtatttcccg atgaaaccac tggttggcat catcatgggc
     2581 tcgacttccg actgggagac gatgcgcgcc gcggcagagg cactcgacaa gctcggcgtt
     2641 ccttacgaga cgagagtggt ctccgcccat cgcacccccg acctgctgtt cgaatacgcg
     2701 tccacggccc agacccgagg catcgaagtc atcgtggccg gagccggcgg cgccgcccat
     2761 ctaccgggca tggtggcctc caagaccacg ttgccggtgc tcggcgttcc ggttcagtcg
     2821 aaggcgctca acggtctcga ctcgctgctg tccatcgtgc agatgccggc gggaattccc
     2881 gtggccacat tcgccatcgg cgtggccggc gccaccaacg cagggcttgc ggcggcggcg
     2941 atcctcgcca acaagcgtcc cgaaatcgac gcggcgctca agaaatttcg cgccgagcag
     3001 acggccaaag tgttggccca ttccgatccc agaatggccg acccttcgaa gctgtgatcc
     3061 ggcaccgatg accattggca tcgtcggcgc cggacagctc gggcgcatga tggccctcgc
     3121 cggctatcca ctcggattcg acttcctgtt cctcgacaag gatgcgcaga cgcc
//
LOCUS       DATDEU010000643         6993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1440667, whole genome shotgun sequence.
ACCESSION   DATDEU010000643 DATDEU010000000
VERSION     DATDEU010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440667"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VIV63_15355"
     CDS             <1..490
                     /locus_tag="VIV63_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004879284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6126029.1"
                     /translation="ADIEFPNDLLRELDRMEFFVHETRELVKARHRPIILITSNNEKE
                     LPDAFLRRCFFHYIRFPDAETMEKIVRVHFPSLKSELLREALTTFFDVRETPGLRKKP
                     STSELLDWIKLLLAEDVPPEALRGDDPKKTIPILHGALLKNEQDVHLFEQLVFLNRRA
                     GR"
     gene            504..1721
                     /locus_tag="VIV63_15360"
     CDS             504..1721
                     /locus_tag="VIV63_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6126030.1"
                     /translation="MLIRFFTDLRAGGVPVTLPEFLSLLEALEARVATLSAEEFYYLA
                     RMAMVKDERHFDRFDRVFAEHFEGAQKLFEKLTAELPPEWLQKLTERLLTEEEKRRVE
                     ALGGWDKLLETLRQRLREQKGRHEGGNKWIGTAGTSPFGNEGFNPEGVRMGQATSRHR
                     RAVKVWDARDYRNFDDGVELGTRNLKMALRRLRRFAREGAAEELDLDGTIDATARNAG
                     LLDLKLVPERRNAVKLLLLLDVGGSMDPHIKVCEELFSAARAEFKHLEYFYFHNFPYE
                     RVWKDNRRRFSVITDTWTLLRTFNPDYRVVFVGDATMSPYEIEQPGGSVEHWNEESGR
                     VWMTRIAKHFPRLVWLNPETEDRWEWTPSVAITKELVAGRMFPLTLEGLDRAMRELKR
                     GNPRPMPGVDPVR"
     gene            complement(1723..2244)
                     /locus_tag="VIV63_15365"
     CDS             complement(1723..2244)
                     /locus_tag="VIV63_15365"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY6126031.1"
                     /translation="MAVTASVCAQSPSAAPAEGSAAPALKLQDQTGKWQTLDQYKGKW
                     VVLYFYPKDFTGGCTTQACELRDNIFAFKKADAVILGVSVDDVASHEKFAKEHSLPFD
                     ILADPTKEVSGKYGVLTTYGTAQLASRQTFLIGPDGKVVKHWAKVDPKGHSDMVLAEI
                     KSHSADKAPAKAS"
     gene            2301..3446
                     /locus_tag="VIV63_15370"
     CDS             2301..3446
                     /locus_tag="VIV63_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY6126032.1"
                     /translation="MCRGEANISSGGSVSRAGLILTGGGARAAYQVGVLKALAEIHES
                     PEWPFPIIVGTSAGAVSASILAGNVTRWHQSVGEIEKVWANFHVAQVFRSDAAAMLKA
                     GARWMGSLFTGGMIGPPKSLLDNSPLRELLSKVADFDAVRANVAQGHLRALALCATSY
                     FTARSVSFFEAETTISEWSRAQRHGERVPLSLDYLMASLGIPFLFPPMSLQGEYFGDG
                     AMRQTAPLSPAIHLGADRILVLGVRPTHLDLIPRFVASDSPPTPGQLFGYMLDTLFSD
                     QISADLENLNRINRLSELAGGSTGTRQIRTMLIAPSVDPSQIAVGHLKSLPVSLRSLM
                     RIIGARGAAGSLLASYLMFESGYTRELIELGYRDTLGQRDELAAFLA"
     gene            3446..4249
                     /locus_tag="VIV63_15375"
     CDS             3446..4249
                     /locus_tag="VIV63_15375"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY6126033.1"
                     /translation="MKPRVLQDNAVHVWLGFETLLDVPGNAERFVALMSDEERVRDQR
                     FLVEPARRLHRLARGMQREVLASYLPGTTPRELAFVSGATGRPALAPPFDTSGIEFNL
                     AHTKGLVVLAVTRGAALGIDVEIYDKKVPLEVARRFFSPAEVDALEALPADAQPHRFL
                     RLWTLKESYLKAVGTGIAGGLDSMTFRIDDAGACAFERADDARAAHWSFSQFDVGARH
                     VMALARLAAQAGRAHADIEWLDFDGASSPATRIEPLLRGASGQEERAQA"
     gene            complement(4220..5578)
                     /gene="mgtE"
                     /locus_tag="VIV63_15380"
     CDS             complement(4220..5578)
                     /gene="mgtE"
                     /locus_tag="VIV63_15380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY6126034.1"
                     /translation="MAESTVIPVKEHRLAALRGALREGSWRGAARMIAAMHPAEIALL
                     IESLPPAQREVVWNFVEPEIEGDVLVELNENVRQSLIDGMDADELLAAAENMELDDLA
                     DLVGDLPEAVSLQLVKSMDQQDRERLNAVLAYEHDTAGGLMNTDTVSVRADVTVEVVL
                     RYLRMRGELPDKTDRLFVVDRNDKYMGTLALTRLLTEDPEKPVGDILDSEGSRISPDT
                     NAHEVAQLFQDRDLVSAAVVDDHGKLLGRITVDDVVDVIREEAAHEVMSAAGLGDEED
                     VFAGILPSTRRRLVWLGINLVTAFMAAAVVSRFEGTIEKVAALAALMPIVASMGGIAG
                     TQTVTLIIRGIALGQVEWVNARWLLWKEIAVGGLNGIIWAVIVGAVTIVWYGTWKIAA
                     IIAAATLINLLAAAAVGVIVPLILRRLKIDPALSAGVILTTFTDCIGFATLLGLGALF
                     LT"
     gene            complement(5691..6599)
                     /gene="rapZ"
                     /locus_tag="VIV63_15385"
     CDS             complement(5691..6599)
                     /gene="rapZ"
                     /locus_tag="VIV63_15385"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY6126035.1"
                     /translation="MRLILVSGLSGSGKSVALHMLEDAGFYCVDNIPAALLKPFISHT
                     LRNNDAQYSRAAVGVDARNAAAEIETVPPLIEELKKSGIQCEIIFLLANDDELLRRFA
                     ETRRKHPISTDHVSLKEAIAIERRLLEPILNAADLVIDTSRMGVHDLRELISQRVEKR
                     SAGRLSLLVESFGFKNGIPGDADFVFDARSLPNPYWDPSLRALTGRDPDVIRFLEAQR
                     SVPRLADDILQFVATRIPEHQAANRRYLTIAIGCTGGRHRSVYLAERIAERLSHNYPV
                     VGIRHNSLGETRSITRAPTLAGAQSA"
     gene            complement(6610..6933)
                     /gene="raiA"
                     /locus_tag="VIV63_15390"
     CDS             complement(6610..6933)
                     /gene="raiA"
                     /locus_tag="VIV63_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY6126036.1"
                     /translation="MQLSLSGHHVEVTPSMRSYVEKKFERISRHFDHVIDVHCVLTVE
                     KLRQKAEATLHVRGSSIHADATEENMYTAIDLLADKLDRSVLKHKEKKADKHAAEARK
                     AVRQE"
BASE COUNT         1185 a         2201 c         2303 g         1304 t
ORIGIN      
        1 ggccgacatc gaattcccca acgacttgct gcgcgagctc gatcgcatgg agttcttcgt
       61 gcacgagacg cgcgagctgg tgaaggcgcg ccaccggccc atcatcctga tcaccagcaa
      121 caacgaaaaa gaactgcccg atgccttcct gcgccgctgc ttctttcact acatccgctt
      181 tccggatgcg gagaccatgg agaagatcgt gcgcgtgcac tttccgtcgc tcaagtcgga
      241 gctgctgcgc gaggcgttga ccaccttctt cgacgtgcgc gaaacgccgg gcctgcgcaa
      301 gaagccgtcg acctccgagc tgctcgactg gatcaaacta ctgctcgctg aggacgtgcc
      361 gcccgaggcg ttacgcggcg acgatccgaa gaagaccatc cccatcctgc acggcgcgtt
      421 gctcaagaac gaacaggacg tgcacctgtt cgagcaactc gtgttcctga atcgccgcgc
      481 gggccgctga ccgcgccgtc gcgatgctga tccgcttctt caccgatctg cgcgccggcg
      541 gcgtgccggt gacgctgccc gaattcctct cgctgctcga ggcgctcgaa gcccgcgtcg
      601 caacgctctc cgccgaagag ttctactacc tcgcgcgcat ggcgatggtg aaggacgagc
      661 ggcacttcga ccgcttcgac cgcgtgttcg ccgaacactt cgaaggtgca cagaaactct
      721 tcgagaagct gaccgcggag cttccgcccg aatggctgca gaaactcacc gagcggcttc
      781 tgaccgaaga agaaaagcgg cgcgtggaag cgctgggtgg ctgggacaaa ctgctcgaga
      841 ccttgcgcca gcggctgcgc gagcagaagg gccggcacga gggcggcaac aagtggatcg
      901 gcacggcggg cacatcgccg tttggcaacg agggattcaa tcccgaaggt gtgcgcatgg
      961 ggcaggccac gtcacgtcac cggcgcgcgg tgaaagtgtg ggatgcgcgc gattaccgca
     1021 acttcgacga cggcgtcgaa ctcggcacgc gcaatctcaa gatggccctg cggcgcctgc
     1081 gccgtttcgc gcgcgaaggc gccgctgaag aactggatct cgacggcacc atcgacgcga
     1141 ctgcacgcaa tgccgggttg ctggacctga agctcgtgcc cgaacggcgc aacgcggtga
     1201 aactgctgtt gctgctcgac gtgggcggct ccatggatcc gcacatcaag gtgtgcgaag
     1261 aactcttctc cgccgcccgc gccgaattca agcatctcga atatttctac ttccacaatt
     1321 tcccgtacga gcgcgtgtgg aaggacaacc gccggcgttt cagcgtgatc accgacacgt
     1381 ggacactgct gcgcacgttc aatcccgact accgcgtggt gttcgtcggc gacgccacca
     1441 tgagcccata cgagatcgaa cagccgggcg gcagcgtgga gcactggaat gaggaatcag
     1501 gccgcgtctg gatgacgcgt atcgcgaaac attttccgcg cctggtatgg ctgaatccgg
     1561 aaaccgaaga tcggtgggag tggacgccgt cggtggcgat cacgaaggaa ttggtcgccg
     1621 ggcgaatgtt tccgctgacg ctcgaaggac tcgatcgcgc gatgcgcgaa ctgaagcggg
     1681 gaaacccccg gccgatgccg ggggttgatc cggttcgcta gatcagctcg ccttggcggg
     1741 cgccttgtcc gccgaatggg acttgatctc ggccagcacc atgtccgaat ggcctttcgg
     1801 atccaccttc gcccagtgct tgacgacctt gccatcgggc ccgatcagga acgtctggcg
     1861 gctcgcgagt tgcgcggtgc cgtaggtggt gagcacgccg tacttgccgg acacctcttt
     1921 cgtcggatcg gcgaggatgt cgaaaggcag gctgtgttcc ttcgcgaatt tttcgtgcga
     1981 ggcgacgtcg tcgacgctca cgccgaggat cacggcatcc gccttcttga acgcgaagat
     2041 gttgtcgcgc agttcacagg cctgggtggt gcagccaccg gtgaagtcct tcgggtagaa
     2101 atagagcacc acccacttgc ccttgtactg gtccagcgtc tgccacttgc cggtctggtc
     2161 ctgtagtttg agcgccggcg cggcggaccc ttccgcgggt gccgcggagg gagactgtgc
     2221 gcatacactt gcggtgaccg ccatcagggc ggtgaccaag atccttcgaa gtgcgagcat
     2281 gttgttctcc gtgaagtgga atgtgtcgtg gggaggcgaa tattagcagc ggtggcagtg
     2341 tttctcgcgc gggcctgatc ctgaccggtg gcggcgcgcg tgccgcttac caggtgggcg
     2401 tgctcaaggc gctcgccgag atccatgaga gtccggagtg gccgtttccc atcatcgtgg
     2461 gcacatcggc tggcgccgtg tcggccagca tcctggccgg aaacgtgacg cgctggcatc
     2521 agtcggtggg cgagatcgag aaggtctggg ccaattttca cgttgctcaa gtctttcgtt
     2581 ccgacgccgc ggcgatgctc aaggccggcg cccgctggat gggttccctg ttcacggggg
     2641 gcatgatcgg cccgccgaaa tccctgctcg acaactcacc gctgcgcgag ctgctgtcga
     2701 aggtcgcgga tttcgacgcg gtacgcgcga acgtggccca gggccacctt cgggcgctgg
     2761 cgttatgcgc caccagctat ttcaccgcgc gctcagtgtc attcttcgaa gccgagacca
     2821 ccattagcga atggtcgagg gcgcagcgcc atggcgaacg cgtcccgctg tcgctcgact
     2881 atctgatggc gagtctcggc attccattcc tgttcccgcc gatgagtctg cagggcgagt
     2941 acttcggcga cggcgccatg cgccagaccg cgccgctgtc gccggccatt cacctgggcg
     3001 ccgatcgcat cctcgtgctc ggcgtgcggc cgacgcatct cgacctgatc ccgcgcttcg
     3061 tcgcctcgga ctcgccaccc acgccgggtc agttgttcgg ctacatgctc gacaccctgt
     3121 tcagcgacca gatttccgcc gatctcgaaa acctgaaccg catcaaccgg ctttcggaac
     3181 tcgccggcgg ttcgacgggc acgcgccaga ttcgcaccat gctgatcgca cccagcgtcg
     3241 acccctcgca gatcgccgtc ggacatctca aatcactgcc cgtcagcctg cgttcgctca
     3301 tgcgcatcat cggcgcccgc ggcgcggccg gcagtctgct cgccagctac ctgatgttcg
     3361 aatcgggtta cacgcgcgaa ctcatcgagc tcggctatcg cgacacgctc ggccaacgcg
     3421 acgaactcgc ggccttcctc gcctgatgaa accgcgggtg ctccaggaca acgccgtcca
     3481 cgtgtggctc ggcttcgaaa ccttgctcga tgttcccggg aacgccgaaa gattcgtcgc
     3541 cctgatgtcc gatgaggaac gtgtgcgcga ccagcgattc ctcgtcgagc ccgctcgcag
     3601 gctgcaccgg ctcgcgcggg gcatgcagcg tgaggtgctc gcgtcctacc tgcccggcac
     3661 cacgccccgt gagctcgcgt tcgtcagcgg cgccacaggg cgtccggcgc tggcgccgcc
     3721 gttcgacacg agcggcatcg agttcaatct ggcgcacacg aaagggctgg tggtgctcgc
     3781 ggtgacgcgc ggcgcggcgc tcggcatcga tgtcgaaatt tacgacaaga aagtgccgct
     3841 cgaagtggcg cggcgattct tttcgccggc cgaagtggat gcgctcgagg ccctgccggc
     3901 agacgcgcag cctcaccgat tcctgcggtt gtggacgctc aaggagtcct atctgaaagc
     3961 ggtgggcacc ggcatcgcgg gcggcctcga cagcatgacg ttccgcatcg acgacgcggg
     4021 cgcgtgtgct ttcgagcggg ccgacgatgc gcgcgcggcg cactggtcgt tcagccagtt
     4081 cgacgtcggc gcgcgccatg tgatggcgct cgcgcggctc gcggcccagg ccgggcgtgc
     4141 gcacgccgat atcgagtggt tggatttcga cggcgcttca tcgcccgcca cccgtatcga
     4201 gcccctgctc aggggcgctt caggtcagga agagcgcgcc caggcctaac aacgtggcga
     4261 agccgatgca gtcggtgaac gtggtcagga tcacgcccgc ggacagcgcc ggatcgatct
     4321 tcaggcgccg caggatcagc ggcacgatca cgcccacggc cgccgcggcc agcagattga
     4381 tcagcgtggc ggccgcaatg atggcggcga tcttccaggt tccgtaccag acgatggtca
     4441 cggcgccgac gatgacggcc cagatgatgc cgttgagccc gcccaccgcg atttccttcc
     4501 acaacagcca gcgcgcgttg acccactcga cctggccgag cgcgatgcca cggatgatga
     4561 gcgtcaccgt ctgagtgccg gcgatgccgc ccatgctggc gacgatgggc atgagggcgg
     4621 cgagggcggc gaccttttcg atggtgcctt cgaagcggct gacgacggcc gcggccatga
     4681 acgcggtgac caggttgatg cccagccaca ccaggcgccg gcgcgtggag ggaagaattc
     4741 ccgcgaacac gtcttcctcg tcgccgaggc cggcggccga catgacttcg tgcgcggcct
     4801 cttcgcggat gacgtcgacg acgtcgtcga cagtgatgcg gccgagcagc ttgccgtgat
     4861 cgtcgaccac ggccgccgac acgaggtcgc ggtcctggaa caattgcgcg acctcgtgag
     4921 cattggtgtc gggcgagatg cgcgagcctt ccgagtcgag gatgtcgccg acgggttttt
     4981 cggggtcctc ggtcagcagc cgcgtgagcg ccagcgtgcc catgtacttg tcattgcgat
     5041 cgaccacgaa cagccggtcg gtcttgtcgg gcaattcgcc gcgcatgcgc aggtagcgca
     5101 gaacgacctc cacggtcacg tcggcccgca cgctcaccgt gtcggtgttc atgaggccgc
     5161 cggcggtgtc gtgttcgtac gcgagcacgg cgttcaggcg ctcgcggtcc tgctggtcca
     5221 tcgacttcac gagctgcaac gacacggcct cgggcaggtc acccacgagg tcggcgagat
     5281 cgtcgagctc catgttctcg gcggcggcga gcagttcgtc ggcgtccatg ccgtcgatga
     5341 gcgactggcg cacgttttcg ttgagctcga ccagcacgtc gccttcgatc tcgggctcga
     5401 cgaagttcca gaccacttcg cgttgcgccg gcggcaacga ttcgatgagc agcgcgatct
     5461 ccgccgggtg catggcggcg atcatccgcg cggcgccgcg ccaggatcct tcgcgcagcg
     5521 ctccccgcaa cgccgcgagg cgatgttctt tcacggggat gacggtgctc tcggccattt
     5581 cggccgggag tgtagtgggg agaatgaaaa aaggggacag atctatttat tgcttcggcc
     5641 agcgccgcca gttttccggc tcagcaataa atagatctgt cccctttttc tcaggccgac
     5701 tgagctccgg ccagtgtagg agcgcgcgtg atcgaacgcg tttcgccgag agagttgtgc
     5761 cggatgccga cgactggata gttgtgcgag aggcgctcgg cgatgcgttc ggcgaggtaa
     5821 accgagcggt ggcggccgcc ggtgcagccg atggcgatgg tgagatagcg gcggttggcc
     5881 gcctgatgtt cgggtatgcg cgtggcgacg aactggagga tgtcgtcggc gaggcgcgga
     5941 acgctgcgct gagcttcgag aaatcggatg acgtcagggt cgcggccggt gagcgcgcga
     6001 agcgacgggt cccagtaagg gttgggcagc gagcgcgcgt cgaacacgaa gtccgcgtcg
     6061 cccggaatgc cattcttgaa accgaaggac tcgaccagca gcgaaagccg accggcgctg
     6121 cgtttttcga cgcgctggct gatgagttcg cgcaggtcgt gcacgcccat gcgcgaggtg
     6181 tcgatgacca ggtccgcggc gttgaggatg ggctcgagca gccggcgctc gatggcgatg
     6241 gcttccttca gcgacacgtg gtcggtgctg atggggtgct tgcgccgggt ttcggcgaaa
     6301 cgccgcagca gttcgtcgtc gttggccagc aggaagatga tttcgcactg gataccgctt
     6361 ttcttcagct cttcgatgag cggcggcacg gtttcgatct ccgccgcggc gttgcgcgca
     6421 tcgactccca ccgccgcgcg cgagtactgc gcgtcgttgt tgcgcagcgt gtgggagata
     6481 aagggcttca gcagcgcggc cgggatgttg tcgacgcagt agaacccggc gtcttcgagc
     6541 atgtgcaaag ccacgctctt gccggacccc gacagcccgc tgacgagaat taggcgcatt
     6601 gagaccggtt cactcctgcc tgacggcctt gcgggcttcg gcggcgtgtt tgtcggcctt
     6661 cttttccttg tgcttgagca cgctgcggtc gagtttgtcg gccagcaggt cgatggcggt
     6721 gtacatgttc tcctcggtcg cgtcggcgtg gatggagctg ccgcgcacat gcaatgtggc
     6781 ttcggccttt tgtcggagct tctcaaccgt caggacgcag tgcacgtcga ttacgtggtc
     6841 gaaatgacgg gaaatgcgtt cgaatttctt ctcgacgtaa gagcgcattg agggggtgac
     6901 ttcgacatga tgaccggaaa gcgacaattg catgatccgt ctcctttttt ggcctatcta
     6961 cagttctgac gttacctgct gcgactatct gtt
//
LOCUS       DATDEU010000644         3528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_417094, whole genome shotgun sequence.
ACCESSION   DATDEU010000644 DATDEU010000000
VERSION     DATDEU010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417094"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VIV63_15395"
     CDS             <1..733
                     /locus_tag="VIV63_15395"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY6126037.1"
                     /translation="RSTARAAGWQWRIPLQHRNGNGYVYSSGHIADEAAASTLLAHLD
                     GRALADPRVLKFTTGRRTKMWSRNCVALGLASGFLEPLESTSIYLIQSGIARLIQMLP
                     GRRMNPVLQDRYNAEAAFEMERIRDFLILHYHATERRDSDFWKYCGTMPIPDSLEQVI
                     RLFRDSGRFYRNSDEMFALTSWVQVMIGQRIVPRAYHPLVDLVAQPDLERLAESVRGV
                     IASCVAAMPPHEQFIERCCKAPVAA"
     gene            730..2235
                     /locus_tag="VIV63_15400"
     CDS             730..2235
                     /locus_tag="VIV63_15400"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY6126038.1"
                     /translation="MMHAIVRARLTSLVVAATCVLLVLACETSKQKPRPEPVRIAPAV
                     PLKALDPLLEDVEKRTFNFFWETTEGATGLVPDRWPAAPFASIAAVGFALNAYAVGVE
                     RGYVTRAQARARVLTTLRFLHEAPQGSDPANSAGYQGFFYHFLNFGSGKRYGNSELST
                     VDTAILMAGVLFAAGYFDQDHADEAQIRKLADELYRRVNWPWSIEHVPDIRMAWSPEG
                     QFQTHDWRGYNEAMLVYILALGSPTFPAARQTWLAWTSDYYRSWGTLEGQTHLTFGPL
                     FGHQYSAIWIDFRGIKDEFMWPLGLDYFENSRRAVLSQRAYAIRNPQDWRGYGPNIWG
                     ITASDGPGDFELPYLGQKRKFRGYSARGVGLESRPEEVFDDGTIAPTAVIGSLPFAEE
                     IVVPATREMHKRYGQYIYGKYGFVDAFNPSFDYDVPLKRGRRVRGVGWVATDYLGIDQ
                     GAIISMIENHRSELMWSVMKRNRYLRRGLTLAGFEGGWLDVPLAGPDISPP"
     gene            complement(2222..>3528)
                     /locus_tag="VIV63_15405"
     CDS             complement(2222..>3528)
                     /locus_tag="VIV63_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY6126039.1"
                     /translation="RGLWFGWDGETVENPADEPAVVRKDGVMYASIEFEQAEYKDFYL
                     GFCNGTLWPLFHYFVDGFRYNDVQYEAYQRMNQRYARQLLPLLEPDDLIWVHDYHLFP
                     LAQKLREAGATQPMGLFLHIPFPNIEVLRALPVYGELLQAMLAYDVLGFQTEPDRDAF
                     RSAVASVWGEKTLTSDHTVTLGDRRVVTGVFPIGVDVDAIQREAVESLSTEACKRMIA
                     GLLGRRLMIGVDRLDYSKGLVERFKAYERFLETHPENQNRVTFLQIAPLSRSDVRAYA
                     EIRRDLEQTTGRTNGRFADTDWTPIRYLNRNFPHDVLMGFMRSALIGIVTPVRDGMNL
                     VAKEFVAAQDPADPGVLILSTMAGAARELGAAVMVNPYDARGMAHAIQQAFNMPIAER
                     RERHQSMVDVLRRNSITAWHTSFVDTLDSVAGRVPELALRVG"
BASE COUNT          628 a         1185 c         1091 g          624 t
ORIGIN      
        1 acgctcgacg gcgcgggccg cgggctggca atggcgtatc ccgcttcagc atcgcaacgg
       61 caatggctac gtctactcga gcgggcatat cgccgacgaa gcggccgcct cgacgttgtt
      121 agcccacctc gacgggcgcg cgctcgccga tccgcgcgtg ctgaaattca cgacgggccg
      181 caggacgaag atgtggtcgc gcaattgcgt cgcgctggga ctcgccagcg gttttctcga
      241 accgctggaa tccacgagca tctatctcat ccagtcgggg atcgcgcgcc tgatccagat
      301 gttgcctggc cggcgcatga atcccgtgct gcaggatcgc tacaacgccg aagcggcatt
      361 cgagatggaa cgcatccgcg acttcctgat cctgcactat cacgccacgg agcggcgcga
      421 ctcggatttc tggaagtact gcggcaccat gccgataccg gattctctcg aacaggtgat
      481 tcgcctgttc cgcgacagcg ggcgcttcta tcggaatagc gatgaaatgt tcgcgctcac
      541 gagctgggtg caggtgatga tcggacagcg catcgtgccg cgcgcctatc atccattggt
      601 cgacctcgtc gcgcagcccg acctcgaaag gctcgccgaa agcgtgcgcg gcgtgatcgc
      661 gagctgcgtc gccgccatgc cgccgcacga gcagttcatc gagcgttgct gcaaggcgcc
      721 ggtggccgca tgatgcacgc catcgtgcga gcccgtctca catccctcgt cgtcgccgcc
      781 acctgcgtac tgttagtgct ggcctgcgag acgtcgaagc aaaaacctcg ccctgaaccg
      841 gtgcgcatcg cgcccgcggt tccgctgaaa gcgctcgatc cgctgctcga agacgtcgaa
      901 aagcgcacct tcaacttctt ctgggaaacc accgaaggcg ccaccggcct cgtgcccgat
      961 cgctggcccg ccgcgccatt cgccagcatc gccgcggtgg gcttcgcact gaacgcctat
     1021 gccgtgggcg tcgagcgcgg ctacgtcact cgcgcccagg cgcgggcacg cgtactcacc
     1081 acgctgcgtt tcctgcacga ggcgccgcag ggaagcgacc ccgccaacag cgccggttac
     1141 cagggctttt tctatcactt cctcaatttc ggcagcggca agcgctacgg aaactccgaa
     1201 ctatccaccg tcgacacggc aattctcatg gctggtgtgt tgttcgccgc gggatatttc
     1261 gaccaggacc atgccgacga ggcgcagatc cgcaaactcg ccgacgaact ctaccggcgc
     1321 gtgaactggc cctggtcgat cgaacacgtg cccgacatcc gcatggcctg gagtcccgaa
     1381 ggacagttcc agacccacga ctggcgcggc tacaacgaag cgatgctggt gtacatcctg
     1441 gcgctgggct ctcccacttt cccggcagcg cggcaaacgt ggctcgcgtg gacgtccgac
     1501 tactatcgaa gctggggcac gctcgaaggg caaacccacc tgaccttcgg gccgctgttc
     1561 ggccaccagt acagcgccat ctggatcgac ttccgtggca tcaaggacga attcatgtgg
     1621 ccgctcggtc tcgactactt cgagaacagc cggcgcgccg tgttgtcgca gcgtgcgtat
     1681 gccattcgca atccgcagga ctggcgcggc tacggcccta acatctgggg cattaccgcc
     1741 tcggatggcc ccggcgattt cgagctcccg tacctcggtc agaagcgcaa gttccgcggc
     1801 tattcggcgc gcggcgtggg gctcgaaagc cggcccgagg aagttttcga cgacggcacc
     1861 atcgcgccca cggccgtgat cggctcactg cccttcgcgg aggaaatcgt cgtaccggcc
     1921 acgcgcgaga tgcacaagcg atacggccag tacatctatg gcaagtacgg tttcgtcgac
     1981 gcgttcaatc ccagtttcga ttacgacgtc ccgctcaagc gtggccgccg cgtgcgtggc
     2041 gtcggctggg tggccaccga ctatctgggt atcgaccagg gcgcgatcat ctcgatgatc
     2101 gagaaccacc gcagcgaact catgtggtcg gtgatgaagc gcaatcgcta tctgcggcgc
     2161 ggcctcacgc tggcgggctt cgaaggcggc tggctcgacg tgccgctcgc cggccccgat
     2221 atcagcccac cctgagcgcg agttccggca cgcgaccggc caccgagtcg agcgtgtcga
     2281 cgaagctcgt gtgccaggcg gtgatcgagt tcctgcgcag gacatccacc atcgactggt
     2341 gacgttcgcg ccgctccgcg atcggcatgt tgaacgcctg ctggatggcg tgcgccatgc
     2401 cacgggcgtc gtaaggattc accatcactg ccgcgcccag ctcgcgcgcc gcgccggcca
     2461 tcgtcgacag tatcagcacg cctggatccg ccgggtcctg cgcggccacg aactccttcg
     2521 ccacgaggtt catgccgtcg cgaaccgggg tcacgatgcc gatgagcgcc gaccgcatga
     2581 agcccatgag cacgtcgtgc gggaaattgc ggttgaggta acggatcggc gtccagtcgg
     2641 tgtccgcgaa gcggccattg gtgcgcccgg tcgtctgctc gagatcgcgg cggatctcgg
     2701 cgtaagcccg cacgtcgctg cgcgacagcg gcgcgatctg caggaaggtc acgcgattct
     2761 ggttttccgg atgtgtctcc aggaaccgtt cgtacgcctt gaaccgttcg acgaggccct
     2821 tgctgtagtc gagccggtcc acgccgatca tgagccgccg tcctaacaat ccggcgatca
     2881 tgcgtttgca ggcctcggtc gacagcgatt cgaccgcctc gcgctggatc gcgtccacgt
     2941 ccacgccgat cgggaacacg ccggtcacga cgcgccggtc accgagcgtc acggtgtgat
     3001 cggaggtgag tgtcttctct ccccacaccg acgccaccgc cgagcggaat gcatcgcgat
     3061 cgggttcggt ctggaaaccg agcacgtcgt aggccagcat cgcctgcagc agttcgccgt
     3121 agaccggcag cgcgcgcaac acttcgatgt tcggaaacgg gatgtgcagg aacagcccca
     3181 tgggctgcgt cgcgcccgct tcgcgcagct tctgcgccag cgggaacagg tgatagtcgt
     3241 gcacccagat gaggtcgtcc ggctcgagca gcggcaacaa ctggcgcgca taacgctggt
     3301 tcatgcgctg gtaggcctcg tactgcacgt cgttgtaacg aaacccgtcg acgaaatagt
     3361 ggaacagcgg ccacaacgtg ccgttgcaga accccaggta gaaatccttg tattcggcct
     3421 gctcgaattc gatcgaggcg tacatgacgc cgtccttgcg caccaccgct ggctcgtcgg
     3481 cgggattctc gacggtttcg ccatcccaac cgaaccacaa gccacggc
//
LOCUS       DATDEU010000645         3079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_857668, whole genome shotgun sequence.
ACCESSION   DATDEU010000645 DATDEU010000000
VERSION     DATDEU010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857668"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1970)
                     /gene="nirB"
                     /locus_tag="VIV63_15410"
     CDS             complement(<1..1970)
                     /gene="nirB"
                     /locus_tag="VIV63_15410"
                     /inference="COORDINATES: protein motif:HMM:NF011565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEY6126040.1"
                     /translation="MSKRIVVVGHGMVGQRFLETLTKDGSGDFAITMLAEEPRLAYDR
                     VNLSKFFSGSTVEDLTLTTREFFDKNGIELYSGDPVATIDRVNRRVTTKSGAEYEYDL
                     LVLATGSSPFVPPIPGRERPGVFVYRTIEDLEAITEAGKNSTRGVVIGGGLLGLEAAK
                     ALKDLGLVTNVVEFAPRLMAVQVDEGGGGLLRSRIEALGVAVHTGKNTTRIEDGESAR
                     HRMIFADGGSLETDVVVISAGIRPRDELARACGIEVAPRGGIAINGDCRTSDESIYAI
                     GECAAYEGRVFGLVAPGYQMARIAAACILGEEREIFAGADMSTKLKLLGVEVGSIGDA
                     HGTSPGARVYSFFDQRKEVYKKLVVDAEGKKLIGAVLVGDTSDYSMWHQTVANAMPLP
                     ENPETMLFPAAAASAGAPPTNALAALPATAQVCSCNNVSKGGICSAIEAGCTTVGALK
                     TKTKAGTTCGGCTPLVTQLLNAELKKRGVNVNTSMCEHFPYTRQQLFHLVRIGELKTF
                     DDVIAKHGKGLGCDICKPAVASIMASCWNEFVLKKRHAALQDTNDYFLANMQKDGTYS
                     VVPRVPGGEITPEKLIVIGQVAKKFGLYTKITGGQRIDLFGAQVHQLPLIWKELIDAG
                     MESGHAYGKALRTVKSCVGSTWCRYGVQDSVGL"
     gene            complement(2137..2931)
                     /locus_tag="VIV63_15415"
     CDS             complement(2137..2931)
                     /locus_tag="VIV63_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126041.1"
                     /translation="MRNRLIAIFTSGPFVLALAALLINDHVLKQSYPGLITGKLSDFA
                     GLAVVALPLFAAFPRQARAIYLGLAGAFLWWKSPASGAFIAFMNELLPLSIGRTVDYG
                     DLLALAVLPACAKFAARGVADVPDMARLRRRALPPILAAALFGVMGTSVIPVRKDFTV
                     NSVESASAFPRDEIVEAIVDIMRKRGFKRREPNPPHWEGAFEGNGVFLTYSFPAPNSV
                     AIGLSANPGMFGRGEAREAENIRKSIERSLSLRFKGLEFVDLVSDR"
BASE COUNT          563 a          988 c          976 g          552 t
ORIGIN      
        1 agaccgacgc tgtcctggac gccatagcgg caccaggtcg aacccacgca gctcttcacg
       61 gtgcgcaagg ccttgccgta ggcgtggccg gattccatgc cggcatcgat gagttctttc
      121 cagatcagcg gaagctgatg aacctgcgcg ccgaagaggt cgatgcgctg gccgccggtg
      181 atcttggtgt agagcccgaa cttcttcgcc acctggccga tgacgatgag tttctcggga
      241 gtgatctcac cacccggcac acgcgggacg acggaatacg tgccgtcctt ctgcatgttg
      301 gccaggaagt aatcgttggt gtcctgcagc gccgcgtggc gcttcttgag cacgaattcg
      361 ttccagcacg aagccatgat ggacgcgacc gccggcttgc agatgtcgca acccaggccc
      421 ttgccgtgtt tggcgatgac gtcgtcgaag gtcttgagct cgccgatgcg cacgaggtga
      481 aacaattgtt gacgcgtgta cgggaagtgt tcgcacatcg acgtgttgac gttgacgccg
      541 cgcttcttca gctcagcatt caacaactgc gtgaccagcg gcgtgcagcc gccgcaggtc
      601 gtgcccgcct tcgtcttggt cttcagcgct ccgaccgtcg tgcagcccgc ctcgatggcc
      661 gagcagatgc cgcctttcga gacgttgttg cacgaacaga cctgggctgt cgccggcagc
      721 gcagccagcg cgttggtcgg gggtgcaccc gcactcgccg ccgctgccgg aaacaacatg
      781 gtttcggggt tctcaggcag cggcatcgca ttggccactg tctggtgcca catcgagtag
      841 tcggaggtat cgccgaccag caccgcgccg atgagcttct tgccctcggc atccaccacc
      901 agtttcttgt agacctcttt gcgctggtcg aagaaggagt acacgcgtgc gcccggcgag
      961 gtgccgtgcg cgtcgccgat ggatcccact tcgacgccca gtagtttgag cttggtgctc
     1021 atgtcggcgc cggcgaagat ctcgcgctct tcgccgagga tgcacgccgc cgcgatgcgc
     1081 gccatctggt agccaggcgc caccaggccg aacacgcgcc cttcataggc cgcgcactcg
     1141 ccgatcgcgt aaatggattc gtccgaggtg cggcaatcgc cgttgatcgc gatgccgccg
     1201 cgcggcgcca cttctatgcc acaggcgcgc gccagctcgt cgcgagggcg gatgcccgcg
     1261 gagatgacca cgacatccgt ttccagcgaa ccgccgtcgg cgaaaatcat gcgatggcgc
     1321 gcggattcac cgtcttcgat gcgcgtggtg ttcttgccgg tgtgcaccgc gacgccgagc
     1381 gcctcgatgc gcgaacgcag aagcccgccg ccgccttcgt ccacctgtac ggccatgagg
     1441 cgcggcgcga attccacgac gttggtgacg aggccgagat ccttgagcgc cttggcggcc
     1501 tcgagtccca gcaatccacc accgatcacg acgccgcgcg tggagttctt gcccgcttcg
     1561 gtgatcgcct cgagatcttc gatagtccgg tagacgaaca cgccgggacg ttcacgaccc
     1621 ggaatcggcg gcacaaacgg gctcgagccc gtggcgagaa ccagcaagtc gtattcgtat
     1681 tcggcgccgc tcttggtggt aacgcgccgg ttgacacggt cgatggtcgc gaccggatcg
     1741 ccggagtaca gctcgatgcc gttcttgtcg aagaactcgc gtgtcgtgag cgtgaggtct
     1801 tcgacggttg atcccgagaa gaacttggat agattgacgc ggtcgtaagc cagccgcggt
     1861 tcctccgcga gcatggtgat cgcgaagtcg ccagaaccgt ccttggtcag tgtttcgagg
     1921 aaacgctggc cgaccatgcc gtgcccgacc acgacaattc gctttgacat tgatcaccat
     1981 ccctcgtcgg taatgagagg gagtaagcaa cctctgtgcc acgccgcctg aaaggcgatg
     2041 ttggtcagcc gggggcctga tttcgcatcg ctgcttcagt tcggtgcaag gggtgcaaac
     2101 gcccgcttca gcagcgggca gcgggacagg acctgcctag cgatcgctca ccaggtccac
     2161 gaactcgagt cctttgaagc gcagagacag cgaacgctct atcgacttgc gaatgttttc
     2221 cgcttcacgc gcttcgcctc gtccgaacat gccgggattg gccgagaggc cgatggcgac
     2281 ggaattgggc gccggaaacg aatacgtgag gaacacgccg ttgccttcga atgcgccctc
     2341 ccaatgcgga ggattcggct cgcgacgctt gaaaccgcgc ttgcgcatga tgtcgacgat
     2401 cgcttccacg atttcgtcgc gcggaaacgc cgatgccgac tcgaccgaat tcaccgtgaa
     2461 gtccttgcgc accgggatga cggacgtccc catgacgccg aacagcgcgg ccgccaggat
     2521 tggcgggagc gcccgtcgcc gcaggcgcgc catgtccggc acatcggcca cgcctcgcgc
     2581 cgcgaacttc gcgcaggcgg gcagcacggc gagcgccagc agatccccat agtcgaccgt
     2641 gcgtccgata ctcagcggca gcagttcgtt catgaaggcg atgaacgcgc ccgaggccgg
     2701 gctcttccac cacaggaacg cgcccgccag gccgaggtag atagccctcg cctgccgcgg
     2761 aaacgcggcg aacaacggca acgcgaccac ggcgagaccc gcgaaatcgg aaagcttgcc
     2821 ggtgatgagc ccgggatacg actgcttcag gacatggtcg ttgatcagca acgccgccag
     2881 cgcgagcacg aatgggccgc tcgtgaagat cgcgataagc cggttacgca tgactgtcct
     2941 cgtgcgttac gtcagggcga gagcgtagaa cctggtcccg ggaatgtgtg cgtcaccata
     3001 cggcggaatc tccacgaatc ccagcacccg gtagagagcg cgcgcggcgg tcatggattc
     3061 gagcgtgtcc agccgcagc
//
LOCUS       DATDEU010000646         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_857771, whole genome shotgun sequence.
ACCESSION   DATDEU010000646 DATDEU010000000
VERSION     DATDEU010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857771"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VIV63_15420"
     CDS             <1..402
                     /locus_tag="VIV63_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6126042.1"
                     /translation="KEKTLLDLTACINVVRHSGRVSIVGYCWGGTLAYIAACELPVFC
                     AVSYYGGQIKDHLDKSPRRPVMYQFGEKDPHIPVSDIDKIRAADPNGIFHLYPADHGF
                     NCDERGTYDAASAALARERTLQFLNAQMERK"
     gene            399..1853
                     /locus_tag="VIV63_15425"
     CDS             399..1853
                     /locus_tag="VIV63_15425"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY6126043.1"
                     /translation="MIKLKDSSLWKELGFIGGQWLPADEGLTTEIRNPANGDRLGTVP
                     VMRAAETRRAIEAAHAAMPAWSKKTAGERAKIMRRWFDLMLANLDDLAVIMTAEQGKP
                     LAESKGEIAYAASFIEWFAEEGKRVYGDIIPGHQADKRIMVLRQPIGVVAAITPWNFP
                     AAMITRKAAPALAAGCTFVVKPAPQTPYSALAMAELAQRAGIPAGVFNVVTGDAVAIG
                     GEFTGNDKVRKVSFTGSTPVGKLLMAQCAGTVKKVALELGGNAPFLVLEDADLDAAVT
                     GAIQSKYRNTGQTCVCANRFIVHSKIYDAFTQKLTEAVDKLRVGDGLKGETDQGPLID
                     AKALAKVENHIADAQAKGATVVTGGKRHALGGTFYQPTVLTNVNTTMTLAREETFGPV
                     APLFRVDSDEEAVRLANDTEFGLAAYVYTRDLTRSWRVTEALEYGIVGLNTGLISTEV
                     APFGGVKESGIGREGSRYGILEFTELKYICVGGI"
     gene            1906..>2317
                     /locus_tag="VIV63_15430"
     CDS             1906..>2317
                     /locus_tag="VIV63_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126044.1"
                     /translation="MLGKFHEISIETADIADSVSFYERLGFSQCGTTDTWTHPYGVMT
                     DGRLYIGLHQFKFPSPTLTYVHPGVAAHSHVIEKLGIDIAWKRVSDEAFNELGFLDPS
                     GQTVRVQEAPTHFASDRGRGETSLCGDFAEYSLPS"
BASE COUNT          443 a          745 c          754 g          375 t
ORIGIN      
        1 aaggagaaga cgcttctcga tctgaccgcc tgcatcaacg tggtcaggca ttcgggtcgc
       61 gtgtcgatcg tgggctactg ctggggtggc acgctggcct acatcgcagc ctgcgagctg
      121 ccggtgttct gtgccgtgtc gtattacggc ggccagatca aggatcacct cgacaagtcc
      181 ccgcgccggc cggtcatgta tcaattcggc gagaaggatc cacacattcc cgtgagtgac
      241 atcgacaaga tccgcgccgc ggatcccaac ggaattttcc acctctaccc ggccgaccat
      301 gggttcaact gcgacgagcg cggcacctac gatgcggcca gcgccgcgct cgcgcgagag
      361 cgcacgctgc aattcctgaa cgcgcaaatg gaacgcaaat gatcaagctc aaagattcat
      421 cgttgtggaa ggaactgggc ttcatcggcg gccagtggct gccggcggac gaaggactga
      481 ctaccgagat ccgcaatcct gccaatggcg atcgcctggg caccgtaccg gtgatgcgcg
      541 ccgccgagac gcgccgcgcc atcgaagccg cgcacgccgc gatgcccgcc tggtcgaaga
      601 agacggccgg cgagcgagcg aagatcatgc ggcgctggtt cgacctcatg ctcgcgaatc
      661 tcgacgacct cgcggtcatc atgaccgccg agcagggcaa gccgctggcg gaatcgaagg
      721 gtgagatcgc ctacgcggcc tcgttcatcg aatggtttgc cgaagagggc aagcgcgtct
      781 acggcgacat cattcccggg caccaggccg acaaacgcat catggtgctg cgccagccga
      841 ttggcgtggt cgcggcgatc acgccgtgga attttcccgc ggcgatgatc acgcgcaagg
      901 ccgcgccggc gctcgccgcg ggctgcacgt tcgtcgtcaa accggcgccg cagacgccct
      961 actcggcgct ggccatggca gagctggcgc agcgcgccgg catccccgcg ggtgtgttca
     1021 acgtggtcac cggcgatgcg gtcgccatcg gtggcgagtt caccggcaac gacaaggtgc
     1081 gcaaggtgtc gttcacgggg tcgactccgg tgggcaaact actcatggcg caatgcgcgg
     1141 gcaccgtgaa gaaggtggcg ctcgagctcg gcggcaacgc accgttcctg gtgctcgaag
     1201 atgccgatct cgatgcggcg gtcacgggcg ccatccagag caagtaccgc aacaccggcc
     1261 agacctgcgt gtgcgccaat cggttcatcg tgcactcgaa gatctacgac gcattcacgc
     1321 agaagctcac cgaggcggtg gacaagctgc gcgtgggtga cggtctcaag ggtgagacgg
     1381 accagggtcc gttgatcgac gccaaggcgc tggccaaggt cgagaaccac atcgcggatg
     1441 cgcaggccaa gggcgccacc gtcgtgaccg gcggcaagcg tcacgcgctc ggcggcacct
     1501 tctaccagcc gacggtgctc acgaacgtga acacgacgat gacgctggcg cgcgaggaga
     1561 cattcggccc ggtcgcgccc ctgtttcgcg tggattccga cgaagaagcc gtgcgtctcg
     1621 cgaacgacac cgaattcggc ctggccgctt acgtgtacac gcgggatctc acgcgttcct
     1681 ggcgagtcac cgaggcgctg gagtacggca tcgtggggct caacaccggc ctcatttcca
     1741 ccgaagtcgc gccattcggc ggcgtgaagg agagtggcat cggccgcgaa gggtcccggt
     1801 acggaatcct ggaattcacc gagctcaaat acatctgcgt gggtggaatc tgacggcggc
     1861 gagtgcatag gcagcgcgcg gcaagatgcg taaactcccg cgccagtgtt aggaaagttt
     1921 cacgaaatca gcatcgagac ggccgacatc gccgactcgg tgagcttcta cgagcgtctc
     1981 gggttcagcc aatgcggcac caccgacacc tggacgcatc cgtacggcgt catgacggac
     2041 ggccggctct acatcggcct gcaccagttc aagttcccgt cgcccacgct cacgtacgtc
     2101 cacccgggcg tcgcggcgca ttcgcacgtc atcgagaagc tgggcatcga catcgcgtgg
     2161 aagcgggtca gcgacgaggc gttcaacgag ttgggctttc tcgacccttc gggccagacg
     2221 gtccgcgtgc aggaagcgcc cacgcatttc gccagtgacc gtggccgcgg cgagacctcg
     2281 ctctgcggtg acttcgcgga atacagcctg ccctcct
//
LOCUS       DATDEU010000647         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1732026, whole genome shotgun sequence.
ACCESSION   DATDEU010000647 DATDEU010000000
VERSION     DATDEU010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732026"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /gene="recQ"
                     /locus_tag="VIV63_15435"
     CDS             complement(<1..1679)
                     /gene="recQ"
                     /locus_tag="VIV63_15435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEY6126045.1"
                     /translation="MNELTADLTPTALAAELKHRFGFDSFRPGQQEVVCDILRGRDLL
                     AVMPTGGGKSLCFQLPALLRPGVCLVISPLIALMQDQVRLLQDNGIDATFINSSLERS
                     EISRRLARLERGELKLLYVAPERLLQAEFESEILPRVHAARGISALVVDEAHCVSDWG
                     HDFRPEYRQINRMRTRFPQVPIAAFTATATERVRQDIVKQLALRDPRIHVASFNRPNL
                     YYAVRPKTRATYPELADLARRDDGAGIVYCLSRKRVDELSAQLQADGIRALPYHAGLD
                     SKTRHDNQESFIRDDAQVIVATIAFGMGINKPDVRWVVHYDLPKTLEGYYQESGRAGR
                     DGEKARCILYFGAGDIRTAEHIIAQKVDPVTFEPLADEQRMARQQLRQVLSYAESGEC
                     RRSVQLRYFGESFTGKCNACDNCLEPRATIERTTEAKQFLSCVARLAQRRERYGVAYV
                     IEILRGAETQRILMRDHASLSVYGIGKHLDVQEWRDLARALLHQGLLAQSQDEYAVVS
                     LNEASWQVLKGERAVHVGESVKPVRGRKGSKALAAKTATGPDNALFESLRT"
     gene            complement(1794..2942)
                     /locus_tag="VIV63_15440"
     CDS             complement(1794..2942)
                     /locus_tag="VIV63_15440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6126046.1"
                     /translation="MTPDHLNRLALLINEQRADVLSRWRKQVRELPSASALDNPTLND
                     HMPGLLDELTGALVRNPGLTIPQALAGQSPTAHGLQRLEEAFDIEEVVAEYNILRGCI
                     HDLADENNVSLQGAAFHTINRVFDQAIGLALQTFATRRAQDVLQRREEYLAFVAHDLR
                     TPLSAISLAGRVLEQLLAPVEARPEVGRMLKALRRNVIHLEKLVSKVIDENTNLKTEV
                     GITLERRQTEIWALVEGLIHDLDPVAGTASTRLINSIPDELLAYADASLLKRIFQNLI
                     ANAIKHTPRGEIVIDAREVADHSGVECTVTDNGTGIEADFIGRVFEKGETDSSDTEGR
                     GLGLAIVKTFIEAHGGTVSVESKSGHGSTFRFFLPAERALDDTGTRPT"
BASE COUNT          570 a         1007 c         1038 g          658 t
ORIGIN      
        1 aacgtgcgca gtgattcaaa gagcgcgttg tccgggcccg tcgccgtctt cgccgcgagg
       61 gccttcgagc ccttgcgacc tcgaactggt ttgaccgact cgcccacgtg aacggcgcgt
      121 tcgcctttca gcacctgcca gctcgcttca ttcaaggaga caactgcgta ttcatcctgg
      181 gactgcgcca gcaggccttg atgcagcaac gcccgcgcga ggtctcgcca ttcctgcacg
      241 tcgaggtgct tgccgattcc gtagacgctc aagcttgcat ggtcgcgcat gaggattcgc
      301 tgggtctcgg cgccgcgcag gatctcgatg acataggcaa cgccgtaccg ctcgcgccgc
      361 tgcgcgagcc gcgcgacgca ggagaggaac tgcttcgcct cggtcgtgcg ctctatcgtt
      421 gcgcgcggct cgaggcagtt gtcgcaggcg ttacatttgc cggtaaagct ctcgccgaaa
      481 tatctgagct gcacggagcg gcggcattcg ccggactccg cataactcag cacctgccgc
      541 agttgctggc gggccatgcg ctgttcgtcg gccagcggct cgaaggtcac cggatcgacc
      601 ttctgcgcga tgatgtgttc ggcggttcga atgtcgccgg cgccaaaata gagaatgcag
      661 cgggctttct cgccgtcgcg gccggcgcgc ccggattcct ggtaataacc ttcgagagtt
      721 ttgggcaggt cgtagtgcac gacccagcgc acgtcgggct tgttgatgcc catgccaaac
      781 gcgatggtgg ccacgatcac ctgcgcgtca tcgcgaatga acgattcctg attgtcgtga
      841 cgcgttttcg aatcgaggcc ggcgtggtac ggcaatgccc ggatgccatc ggcctgcagt
      901 tgcgcggaaa gctcgtccac gcgcttgcgc gagaggcagt agacgatgcc ggcgccgtca
      961 tcgcgccgcg ccagatcggc cagttccggg taagtagcgc gggtcttcgg gcgcacggcg
     1021 taataaagat tggggcgatt gaaactcgcg acgtggatgc gcggatcgcg cagcgcgagt
     1081 tgcttcacga tgtcctgccg cacccgctcc gtcgccgtgg ccgtgaaagc agcgatgggc
     1141 acctgcggaa agcgcgtgcg catgcgattg atctggcgat attccggacg gaagtcgtga
     1201 ccccagtccg acacgcaatg cgcctcgtcg acgaccagcg cggaaatgcc gcgcgcggca
     1261 tgaacgcggg gcaggatttc gctttcgaac tcggcctgca gcaaccgttc gggcgcgacg
     1321 tacagcagct tgagttcgcc tcgctccagg cgcgcgagcc gccgcgaaat ttcggatcgt
     1381 tcgagcgacg aattgatgaa cgtggcgtcg atgccgttgt cctggagcag ccgcacctgg
     1441 tcctgcatca acgcgatcaa cggcgagatc acgaggcaaa cgccgggacg aagcagggcg
     1501 ggcaattgaa aacacagcga cttgccgccg ccggtgggca tgacggccag caggtcgcgg
     1561 ccgcgcagga tgtcgcacac cacttcctgc tgtccggggc gaaaggagtc gaagccgaag
     1621 cgatgcttga gctcggcggc gagggcggtg ggggtcaggt cggcggtcag ctcgttcatt
     1681 ccgtccatca taatctcgtc ccgcagcgca catacgcctt gaatattggc gtgcgcagta
     1741 catttcgcgg tcacggtgat cgccatcagg gcctgccgac cgccccgact ttgttaggtg
     1801 ggtcgcgttc cggtgtcgtc gagtgcacgc tccgcaggca ggaagaacct gaaagtggaa
     1861 ccgtgcccgg atttgctctc gaccgagacc gttccgccat gcgcctcgat gaacgtcttc
     1921 acgatcgcaa ggcccagacc cctgccttcg gtgtcactgc tatccgtttc gcccttctcg
     1981 aataccctgc cgatgaagtc ggcttctatg ccggtgccat tgtcggtcac ggtgcattcc
     2041 actccgctgt gatcggcaac ttcgcgagcg tcgatgacga tttcgccgcg tggcgtgtgt
     2101 ttgatcgcat tggcgatcag attctggaag atccgcttga gaaggctggc atccgcgtaa
     2161 gccagaagtt cgtccgggat actgttgatc agacgcgtgc tggcggttcc cgccaccgga
     2221 tcgaggtcgt ggatcagccc ctcgacgagc gcccagattt cggtctggcg gcgttctagt
     2281 gtgattccga cctcggtctt gagattggtg ttctcgtcga tgactttgct gaccagcttt
     2341 tcgagatgaa tgacgttgcg gcgcagcgcc ttgagcattc gtcccacttc gggccgcgcc
     2401 tccactggtg cgagcagctg ctccaggact cgtccagcca gtgaaattgc gctcaagggt
     2461 gtgcgcaaat cgtgagcgac gaacgccaga tattcttccc gacgctgcag cacatcctgc
     2521 gctcgccgcg tggcaaaggt ctgcaacgca agtccgatgg cctgatcgaa tacgcgattg
     2581 atcgtgtgaa aggctgcgcc ctgcaggctc acgttgtttt catcggcgag gtcgtgaatg
     2641 cagccgcgca gaatgttgta ttccgccacg acttcctcga tgtcgaaggc ctcttcgagg
     2701 cgttgcagtc cgtgcgcggt cgggctctga cccgcgagcg cctgcgggat cgtcaagccc
     2761 gggttgcgca cgagcgctcc cgtcagttca tcgagaagtc ccggcatgtg atcgttgagc
     2821 gttggattgt cgagtgcact cgccgaaggc agctcccgca cctgtttccg ccaccgcgac
     2881 agcacgtccg cacgttgctc gtttatcagc aacgccagtc gattcaggtg gtcgggtgtc
     2941 atggggaatc tcactgggtc cgaaatgggt ccaggcgaac cactcgccag catactgcga
     3001 ggcggcgagc ccgaggcggg agatccggat tgtcaggaga ttcaggccgt acgcacgccc
     3061 aggttcggcg aagacgggca catacgatgc gaatctcggc gcgccgcgtt cgtatggact
     3121 taccttgcgg gtcaccttct aaaaaagagt gctcgattct tgtgctggcg ccgattgcga
     3181 agtcggcgcc agcgcattcc tgaacgtcgc ttccctgccg cactgcgtcg cgccatcgtc
     3241 cgatagtggc gttgtacgac ccgcgctaca tcc
//
LOCUS       DATDEU010000648         4399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1586515, whole genome shotgun sequence.
ACCESSION   DATDEU010000648 DATDEU010000000
VERSION     DATDEU010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586515"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VIV63_15445"
     CDS             <1..400
                     /locus_tag="VIV63_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126047.1"
                     /translation="ELSWPADLSGDLAQDLAGQFDLETQGRDSGHQLLASATLAGGQI
                     ALANVQGTGPAADQVFRGSGRVALLTREYDLTVDYERVSLAASAVPTQARARVARAWT
                     ALRGSVARRGWAEEPEARRVQWHGTWDAEH"
     gene            528..1343
                     /locus_tag="VIV63_15450"
     CDS             528..1343
                     /locus_tag="VIV63_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY6126048.1"
                     /translation="MRVAAIQMTSGHDIEANLEAAGRFLEDAAKSEATLAALPENFAF
                     MGLNSGDKRAIAEPDGAGRIQNFLSDTARRLRIWIVGGTVPLRQGGDGRVAASCLVYK
                     SDGQRAARYDKIHLFDVDVPGSTESHRESAHTVAGRDPRVVDTPIGKLGLAVCYDMRF
                     PELFRVMSRQGADMFVVPSAFTVPTGKAHWESLLRARAIENLCGLIAPAQWGRHPNGR
                     ETYGDSMIIDHWGNVVARKAEGTGCVVADIDTAGRMDARARFPALEHRVIYNT"
     gene            1360..1674
                     /locus_tag="VIV63_15455"
     CDS             1360..1674
                     /locus_tag="VIV63_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126049.1"
                     /translation="MTVLVIRRLPGSQDGEYSYRVMVDGRQRAEVGNDSTVQIGLTPG
                     EHRVSLGVKWCGSRELPFTIQAGEIVRMECRPNVRPLLALLYITLWRNRYISLETVKS
                     WT"
     gene            1671..3119
                     /gene="tldD"
                     /locus_tag="VIV63_15460"
     CDS             1671..3119
                     /gene="tldD"
                     /locus_tag="VIV63_15460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY6126050.1"
                     /translation="MSNSVEPLAIARELILRPGGLDDSRLDIALSQVMSSSVDSADLY
                     FQLSREESWALEDGIVKEGSASIEQGVGVRAVAGEKTGFAYSDEIVLPALEEASRAAR
                     AIALRGSERPMHGVLPTNGHRLYLPIDPVSSFTSSEKVAWLERVDRETRKMDPRVKQV
                     MASVVAVHEVVLIANSEGHLAADVRPLVRLNVSVIVEQDGRREQGYAGAGGRITLPEL
                     VANDKPLALAREAVRQALVNLDAVPAPAGLMTVVLGSGWPGILLHEAIGHGLEGDFNR
                     KGTSAFAGRVGERVASELCTIVDDGTLSRRRGSLNIDDEGTPTQCTTLIENGVLKGYL
                     QDKMNARLTGTASTGNGRRESFAHITLPRMTNTYMRPGQTPPEEIIASVQKGIYAVNF
                     GGGQVDITSGKFVFSASEAYLIENGKVGAPIKGATLIGNGPDVLTRVSMVGNDLKLDD
                     GVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTG"
     gene            complement(3129..3626)
                     /locus_tag="VIV63_15465"
     CDS             complement(3129..3626)
                     /locus_tag="VIV63_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126051.1"
                     /translation="MKKLVLLLLLVLAGVYGVGRMKLGEAGAMRFLADMEALMNDGNA
                     EEVCAMFHEDLEVEISDRSGDSPQEMSGDKEEFCALTTETAAGLHLLPHSMSVNYTDV
                     NVTRNWLHPWTSEVSYLENRSLTIRGANVTLRTVSEDEITLVHTFSGVKLLKLKSDVH
                     RADAT"
     gene            3921..4091
                     /locus_tag="VIV63_15470"
     CDS             3921..4091
                     /locus_tag="VIV63_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126052.1"
                     /translation="MRNDTSASGEFRINLPPDVLAEMRRQTGTARSTMQVDGFRPGLF
                     GRLMDLFTGRRR"
     gene            complement(4164..>4399)
                     /locus_tag="VIV63_15475"
     CDS             complement(4164..>4399)
                     /locus_tag="VIV63_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY6126053.1"
                     /translation="IEDLNSTNGIMVRGKRVRRHSLRDGDVVTIGQHEILYVNEHSGH
                     YADTHDDLPRVDVDAANEDADDDSAPEDAAGAR"
BASE COUNT          780 a         1428 c         1416 g          775 t
ORIGIN      
        1 cgagctgtcg tggcccgccg atctttccgg cgatctggcg caagacctgg cagggcagtt
       61 cgatctcgaa acacaagggc gcgacagtgg ccatcaactg cttgccagcg cgactctcgc
      121 cggcgggcag atagccctcg cgaacgtgca ggggacgggc ccggccgccg accaggtctt
      181 ccgcggcagc ggccgcgtgg cgttgctgac ccgggaatac gatctcaccg tcgattacga
      241 gcgcgtctcg ctggccgcca gcgccgtgcc gacgcaggca cgcgcacgcg tggcccgcgc
      301 ctggaccgcg ctccggggct ctgtcgcgcg ccgtggctgg gccgaagagc ccgaagcgcg
      361 ccgcgtgcag tggcacggca cctgggacgc ggagcactaa tggggacatt cctgatttgc
      421 agcaaaaggg gacgcgcctc gtagacgcat ctgcgcactg gtttttcgcg actgaggcgc
      481 gtcccctttt gctagcaaat caggaatgtc cccgatagat ttgccgcatg cgtgttgcgg
      541 caatccagat gacctccggt cacgacatcg aggccaatct cgaagcggcc ggccgttttc
      601 tcgaggacgc cgcgaagagc gaggcaacgc ttgcagccct gccggaaaac ttcgccttca
      661 tggggctgaa cagtggcgac aaacgcgcga tcgccgagcc cgatggtgcg ggccgaatcc
      721 agaacttcct ctccgacacc gccaggcggc tcaggatctg gatagtcggt ggcacggtgc
      781 cgctgcgcca gggcggcgac ggacgcgtcg cggcgtcctg cctcgtctat aaatccgacg
      841 gccagcgcgc agcgcggtac gacaagatcc acctgttcga cgtcgatgtg ccgggcagca
      901 ccgagtcgca tcgcgaatcg gcgcacacgg tcgccgggcg cgatccgcgc gtggtggaca
      961 cgcccatcgg caaactcgga ctcgcggtct gttacgacat gcgttttccc gagctgttcc
     1021 gcgtgatgtc acggcagggt gccgacatgt tcgtcgtgcc ttcggcattc acggtcccca
     1081 cgggcaaggc acattgggaa tcgctgctgc gcgcccgggc catcgagaac ctctgcgggc
     1141 tgatcgcgcc ggcgcagtgg ggcagacatc cgaacgggcg cgagacctat ggcgattcga
     1201 tgatcatcga tcactggggc aacgtggtgg ctcgcaaggc cgaaggaacc ggctgcgtgg
     1261 tcgcggatat cgacaccgcg ggccgcatgg acgcccgcgc gcgttttccg gcgctcgagc
     1321 accgggtgat ctacaacacc tgacgcggag gccgaagccg tgaccgtgct cgtgattcga
     1381 cgcttgcccg ggtcccaaga tggcgaatat tcctaccgcg tcatggtcga cggacggcaa
     1441 cgcgcggagg tcggcaacga ttcgaccgtc cagatcggcc tcacgccggg tgaacatcgc
     1501 gtgagcctgg gcgtcaaatg gtgcggatcg cgcgaattgc ccttcacgat tcaggcgggc
     1561 gagattgtgc gcatggaatg tcgccccaat gtcaggccct tgctggcgct gctgtacatt
     1621 acgctctggc gcaaccgcta catttctctc gaaacggtga aatcttggac ttgagcaatt
     1681 ccgtcgaacc gctggccatt gcccgtgaac tcatccttcg cccgggcggc ctcgacgatt
     1741 cgcggctcga cattgccctg agccaggtca tgtcgagctc ggtcgactcc gcggacctct
     1801 acttccagct ctcgcgtgaa gagagctggg cgctcgagga cggcatcgtc aaggaaggca
     1861 gcgccagcat cgagcagggc gtgggcgtcc gcgcggtggc cggcgaaaag accgggttcg
     1921 cctactcgga tgaaatcgtg ctgcccgcgc tcgaggaagc gtcgcgcgcc gcgcgcgcca
     1981 tcgccctgcg cggcagcgag cggccgatgc acggcgtgtt gcccaccaac ggtcaccgtc
     2041 tctatctacc catcgacccg gtgtcgagct tcacttccag cgagaaggtg gcgtggctcg
     2101 agcgcgtgga ccgcgagacc cgcaagatgg atccgcgcgt caagcaggtc atggcgagcg
     2161 tcgtggcggt gcacgaagtc gtgctgatcg ccaacagcga aggacacctg gctgcggacg
     2221 tgcgcccgct cgtgcggctc aacgtctcgg tcatcgtcga acaggatgga cgccgcgaac
     2281 agggctacgc cggcgcgggt ggccgcatca cgctgccgga gctcgtggcc aacgacaaac
     2341 cgctggcgct tgcgcgggaa gccgtgcgcc aggcgctggt gaatctcgac gccgtgcccg
     2401 cgcccgccgg tctcatgacc gtggtgctcg gctccggctg gcccggaatc ctgctgcacg
     2461 aagccatcgg ccacggactc gagggcgact tcaaccgcaa ggggacatcg gcattcgcgg
     2521 gccgcgtggg cgaacgcgtc gcgtcggagc tgtgcacgat cgtggacgac ggcacgctgt
     2581 cgcgccgccg tggctcgctc aacatcgacg acgaaggcac acccacgcaa tgcaccacgc
     2641 tcatcgagaa cggcgtgctc aagggttacc tgcaggacaa gatgaacgcg cgcctcacgg
     2701 gcacggcgtc caccggcaac ggccggcgtg agtccttcgc gcacatcacg cttccgcgca
     2761 tgaccaacac ctatatgcgg cccggccaga caccgcccga ggagatcatc gcgtcggtgc
     2821 agaagggtat ctacgccgtg aatttcggcg gcggccaggt ggacatcacc tcgggcaagt
     2881 tcgtgttctc ggccagcgag gcttatctca tcgagaacgg caaagtgggc gcgcccatca
     2941 agggcgccac gctcatcggc aacggacccg atgtcctgac gcgcgtgtcc atggtcggca
     3001 acgacctcaa gctcgatgac ggcgtaggca cctgcggcaa ggaagggcag agcgtgcctg
     3061 tcggcgtggg tcagcccacg ttgcgtatcg acggcctcac ggtcggcggc accggctagg
     3121 acttgcgcct aggtcgcgtc cgccctgtgg acgtcgctct tgagcttcag aagcttcaca
     3181 cccgagaagg tgtgcaccag cgtgatctca tcctcgctca ccgtacgcag cgtgacgttg
     3241 gcgccgcgga tggtcaggct gcgattctcg aggtagctga cctcgctggt ccagggatgg
     3301 agccagttcc gggtcacgtt cacgtcggtg taattcacgc tcatcgaatg gggcagcagg
     3361 tgcaggcccg ccgcggtctc ggtggtgagc gcgcagaatt cttccttgtc gccgctcatt
     3421 tcctgcggtg aatcgccgga gcgatcgctg atctccactt cgagatcctc gtggaacatg
     3481 gcgcacacct cctcggcgtt gccgtcgttc atgagcgctt ccatgtcggc gaggaaacgc
     3541 atggcgccgg cttctccaag cttcatgcgg ccgacgccgt agacgccagc caggaccaga
     3601 agcagcagca gaacgagttt cttcatgagc gcactctaag gtgaacgttt ttgcgcattc
     3661 gcgtcggtat tcacgttacc gggcattcgc tgcgcacccg cactattgcg atgcgcggtc
     3721 gacagggatg tcgtcaaagt gcgtcagcat tttgtagcga aagaatcgct ccgcgcctac
     3781 acgcgaatca gacgcgtaca taatgaagtg cgcgttttgc gacattggaa acaatgtgaa
     3841 tcccggcaca tcatggctcc cacatgctta gccacactgc caccgcggtc tcaactgatc
     3901 ctcaccggag ccaatcgaca atgcgtaacg acacttctgc cagcggcgaa tttcgtatca
     3961 acctgccgcc ggacgtgctc gcggaaatgc gccgtcagac gggcaccgcg cgcagcacca
     4021 tgcaagtcga tggctttcgt cccggcctgt tcggccgtct gatggacctt ttcacgggtc
     4081 gccgccgcta acccacagac aacctcaaac aaaaaggcgc gcaagccaaa cggtttgcgc
     4141 gcctttttta ttgcgtcggg ttttcagcgc gcgccggcgg cgtcttccgg ggcggagtcg
     4201 tcatccgcgt cttcgttggc ggcatccacg tccacgcgcg gaaggtcgtc gtgcgtatcg
     4261 gcgtaatgcc cgctgtgctc attgacgtac aggatttcgt gctgaccgat cgtgaccaca
     4321 tcgccatcgc gcagcgaatg ccggcgcacg cgtttgccgc gcaccatgat tccgttggtg
     4381 ctgttcaggt cttcgatga
//
LOCUS       DATDEU010000649         2761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_272615, whole genome shotgun sequence.
ACCESSION   DATDEU010000649 DATDEU010000000
VERSION     DATDEU010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272615"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VIV63_15480"
     CDS             <1..700
                     /locus_tag="VIV63_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6126054.1"
                     /translation="FAPWFAIGTILRQRDAFAREQALEFSLQRSEFERRESDARLRLL
                     QAQVEPHFLFNTLANVQALVEAGSPQASKVLNSLIAYLRAAVPRMHSNTSTLETETDL
                     ARSYLELMQMRIPDRLQYAIHLDPAAARLDCPPMTLLTLVENAVRHGIDPSEAGGRID
                     ADIWIRDERCVIRVKDTGVGLKTVSRGLGTGLSTLRERLKLAFAGDAQLTLTEVVPHG
                     VCAELSIPARKVAA"
     gene            697..1479
                     /locus_tag="VIV63_15485"
     CDS             697..1479
                     /locus_tag="VIV63_15485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY6126055.1"
                     /translation="MTTRVTALIADDEPLLREALVRLLASAWPDLEIVAQARNGREAI
                     DLFDAHKPDICFLDVQMPGKTGVEAAQHIGRRAHLVFVTAFVEYAIQAFERGALDYLV
                     KPVEPERLAATVQRLQERLGASQPAMDTEALLQQLAQALNPDSAPKSLRWVRASVGTT
                     VRLIAVQDIDFLRAEEKYTQVAWRDDAGQPREAVIRTALKDLIPQLDAAEFAQVHRSV
                     VVNLRAISHVTRGSNETADIHLKGRQETLPVSRTYLHLFRQM"
     gene            complement(1493..>2761)
                     /locus_tag="VIV63_15490"
     CDS             complement(1493..>2761)
                     /locus_tag="VIV63_15490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6126056.1"
                     /translation="ASLSLSVSRFLRGSILKEMREFAAAIDQFEKARQLSVDLNDKQG
                     IAFADLDICQVQIEIGQLTEARKRCDNALRIFSASGSRDLVKQTRAELAHIDLEEGAA
                     ARALTTLNDLLKNGASDVPPRDVGPIFKLRSRANAALGNFREAHADLDESVRRTTAAD
                     EARRIQQVATLRARFETNRQLERNAELNRELATAQSRQHEQKRWTAIAIGTGALIITL
                     LGIHAFSIRRHRRQLALLANLDSLTGLPNRRHTDELGQAAMARASAAGQPLTVAVVDL
                     DHFKSINDRCGHAGGDKVLKEFSRVCRESIRGSDVIGRWGGEEFLIVMPGATLDVALS
                     ALERLRAVAMRIELPASGAGLQVGLSAGLASLEVNIKTLDELIARADSALYKAKHEGR
                     DLVRISEEDFEAASSGVRRALRGGAGHPKS"
BASE COUNT          455 a          893 c          934 g          479 t
ORIGIN      
        1 gtttgcgccg tggttcgcca tcggcaccat cctcaggcag cgcgacgcct tcgcgcgcga
       61 gcaggcgctg gagttttcgc tgcaacgaag cgagttcgag cggcgcgaat cggacgcgcg
      121 gctgcggctt ttgcaggcgc aggtcgagcc gcatttcctc ttcaacacgc tggcgaatgt
      181 gcaggcgctg gtggaggcgg gctcgccgca ggcgtcgaag gtgctcaata gtctcatcgc
      241 ctatctacgg gcggccgtgc cgcgcatgca ctcgaatacc agcacgctcg agacggaaac
      301 ggatctggcg cgttcgtacc tcgagctcat gcagatgcgc atcccggacc ggctgcaata
      361 cgcgattcat ctcgatccgg ccgcggctcg actcgattgc ccgccgatga cgctgctgac
      421 gctggtcgag aatgccgttc gtcacggcat cgatccgagc gaggcgggcg gccgcatcga
      481 cgccgacatc tggatccgcg atgaacgctg cgtgatccgt gtgaaggaca cgggcgtcgg
      541 gctcaagacc gtgagccgcg ggctgggcac gggactttca acgctacgcg agcgtctcaa
      601 gctggcgttt gccggcgacg cgcagctcac gctgaccgaa gtggttccac acggggtgtg
      661 cgccgaactc agtattcccg cgcgcaaggt ggccgcatga ccacgcgcgt caccgcgttg
      721 atcgccgacg acgaaccgct gctgcgcgaa gcgctggtgc gcctgctcgc gagtgcgtgg
      781 cccgacctcg agatcgtcgc gcaggcacgc aatggccgcg aagccatcga cctgttcgac
      841 gctcacaaac ccgacatctg ttttctcgac gtgcagatgc cgggcaagac cggcgtggaa
      901 gccgcgcagc acatcggccg gcgcgcacac ctggtgttcg ttaccgcgtt cgtcgaatac
      961 gccatccagg cattcgagcg gggcgcgctc gactacctgg tgaagccggt ggagcccgag
     1021 cgccttgccg cgacggtgca gcgtttgcag gaacggctcg gcgcctcaca accggccatg
     1081 gacacggaag cgctgctgca gcagctcgcg caggcgctca atccggattc cgcgccgaag
     1141 tcgctgcgtt gggtacgcgc atcggtggga accacggtgc ggctgatcgc cgtgcaggac
     1201 atcgacttcc tgcgcgccga ggagaaatac acgcaggtcg cgtggcgcga tgacgccggt
     1261 cagccacgcg aggccgtgat ccgcacggcg ctcaaggatc tgattccgca gctcgacgcc
     1321 gcggagttcg cgcaggtgca tcgttcggtg gtggtgaatc tgcgcgcgat cagtcacgtg
     1381 acgcgcggtt cgaatgaaac cgcggacatt cacctgaagg ggcggcagga gacgctgcct
     1441 gtgagccgca cctatctgca cctgttccgg cagatgtgat ccgccggccc aactacgact
     1501 ttgggtgccc ggcaccaccg cgaagcgcac ggcgcactcc gctcgatgcc gcttcgaaat
     1561 cttcttcgga gatccgcacg aggtcgcggc cttcgtgctt ggccttgtag agcgcggaat
     1621 cagcgcgtgc gatgagttca tcgagcgtct tgatgttcac ttcgagcgag gccaggcctg
     1681 cgctcaggcc cacttgcagt cctgcgcccg aagccggcag ctcgatgcgc atcgccacgg
     1741 cgcgcagccg ctcgagcgca ctcagcgcga catccagcgt cgcgccgggc atcacgatga
     1801 ggaattcttc accgccccaa cgtccgatga catccgagcc tcgaatggac tcgcggcaca
     1861 cgcgcgagaa ttccttcagc accttgtcgc cgcccgcgtg tccgcagcgg tcgttgatgc
     1921 tcttgaagtg atcgaggtcg acgacagcga cggtcagcgg ctgcccggcc gccgaggcac
     1981 gcgccatggc cgcctggccg agttcatcgg tgtgacgccg gttgggcagg ccggtcaggc
     2041 tgtcgagatt cgcgagcagc gcgagctgcc gacggtgccg gcgaatcgag aatgcatgga
     2101 tgccgagcag cgtgatgatg agcgcgcccg tgccgatcgc gatcgccgtc cagcgcttct
     2161 gctcgtgctg gcgcgactgt gcggtcgcga gctcgcgatt gagctcggcg ttgcgctcga
     2221 gctgacggtt ggtctcgaag cgcgcgcgca gcgtggccac ctgttgtatg cgccgcgcct
     2281 cgtcggcggc ggtcgtgcgg cgaaccgact cgtcgaggtc cgcatgcgct tcgcgaaaat
     2341 tgccgagagc cgcgttggcg cgcgaacgca gcttgaagat cggccccacg tcgcgcggcg
     2401 gcacgtccga agcgccgttc ttcagcaggt cgttcaacgt cgtgagcgcg cgcgcggccg
     2461 ctccttcttc gaggtctatg tgcgcgagtt cggcgcgagt ctgcttgacc aggtcgcggg
     2521 atccggacgc ggagaagatg cgcagggcat tgtcacagcg tttgcgcgct tcggtcagtt
     2581 ggccgatctc gatctgcacc tggcagatgt cgaggtccgc aaacgcgatg ccttgtttgt
     2641 cgttgagatc gacgctgagt tggcgcgcct tctcgaactg gtcgatcgcc gcggcgaact
     2701 cgcgcatttc cttgaggatc gaaccgcgca ggaatcggga gaccgagaga gacagcgaag
     2761 c
//
LOCUS       DATDEU010000650         5196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1150687, whole genome shotgun sequence.
ACCESSION   DATDEU010000650 DATDEU010000000
VERSION     DATDEU010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150687"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VIV63_15495"
     CDS             <1..1070
                     /locus_tag="VIV63_15495"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY6126057.1"
                     /translation="VANDKHLGVSISFSAAPAGLPGPPSPGELERIRKENGAIAKLEV
                     LDGNVGYMRVNGAPALEGSRPAVTAAFAFLHDTDALIIDNRGNGGGDPNTAAFYESYL
                     SEGKPHLAGSFHFREGNRVQEFWTTDLGEHSYGARKPVFVLTSVRTFSAGEGIAYNLQ
                     ALKRAVIVGEKTAGGATSPLRMPMGNQFVLAVPVATGVNPITRTSWEGVGVIPDVPVE
                     EAIALSKAHTLAIERLRASVANDRERSAFDSILMKIQSIEEAGSAKTIRLPATDLVGA
                     YVPVVGTGTTVVIFERDGQLTWHVDGAQDRALLHLGGNRYQREGLPSGYTISFRKQSG
                     KTELLMEDPIRPSVIRAKSKS"
     gene            1252..2727
                     /locus_tag="VIV63_15500"
     CDS             1252..2727
                     /locus_tag="VIV63_15500"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEY6126058.1"
                     /translation="MNSHDAEQQELARAIGLLGSNTRHARLLAYVGARYFQGQEAELT
                     EFKIATEVFGRSAQRFDATEDAVVRVEVHRLRKKLREIYEKDTRTQGLQISLPPGTYV
                     PSFTPLSAPPPASDAPVSEPASRWRRIPKWTILTVVIVSAAIASLMIRDGSQPGQTAV
                     PGAAPAQTQSPERAAPGAISELHILAGYHGSEVIDNSGVRWTPDRYFASGGQWSRDFG
                     LVRGTSRPFLFANWRTGEFGYDIPVKPGVYELRLFFVSPYRIGEEKLGGFQVALHGKP
                     LLQDYDINESANGTDIADELVFRDVAPGEDGFIRLWFSNHVASPLLNALELVPGTPGK
                     LHPIRILTQPTSFVDHKGQRWRADDYYLNGFRSTERRKVSGTEDPELFGAERFGHFSY
                     AIPVDRRGRYTVILHFAELYYGAQLAGAGGVGSRVFHVFCNGQTLLRDFDIYKEAGSL
                     RVVTKTFTNIVPSALGKINLDFEPVVNNATVSAIEVIEESP"
     gene            complement(2743..4278)
                     /locus_tag="VIV63_15505"
     CDS             complement(2743..4278)
                     /locus_tag="VIV63_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY6126059.1"
                     /translation="MTRISRRSLLGTPLYAALAASAVARAAASQLPVARGPFQPTADS
                     LKTYQAPDWFRDATFGIWAHWGPQAGPRSGDWYARWMYVPGHPHYAHHLKHYGHPSEF
                     GYKDIIPLWKAEKFDPEALMDRYVAAGAKYFVSMAVHHDNFDLWNSRHHRWNALAMGP
                     RRDIVGAWRDAARRRGLRFGVSEHLGASHNWWYPSHQYDQFWPKFGVDYDGANPAHAD
                     LYHPRHDEVYKGNAQTWYTTNAAFHQVWFNRIRDLVDSYAPDLLYSDGGMPFGEVGRS
                     LLAHFYNSSAAKRGRVDVVYNCKALGSGEFYPEACVQDVERGVMSGINPRPWQTDTSN
                     GDWYFKDGDEYKTASDVIRMLADIVSKNGNLLLNVALYPDGSLPPESQTLLTELAPWM
                     AVNAEAIHGTRPWAVHGEGPTETAAGEFKENAGYKAEDIRFTTKGKMLYAITLGEPRG
                     RVAITSLGRSAGHEKRAVRSVHLLGVRTSLKFEQTDAALIIELPDRLPTRHASAFRIS
                     LGT"
     gene            complement(4288..>5196)
                     /locus_tag="VIV63_15510"
     CDS             complement(4288..>5196)
                     /locus_tag="VIV63_15510"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6126060.1"
                     /translation="KLASGAQVEGELFLDCSGFRGLLIEQALGTGYEEWTHWLPCDRA
                     VAVPCESVSPLLPYTRSTAHRAGWQWRIPLQHRTGNGHVFSSAHMSEDEATAVLLANL
                     DGRPTAEPRTIKFVTGRRRLAWNRNCIAVGLASGFIEPLESTSIHLIQTMIARLMVFF
                     PDLRFSTPDIAEFNREAQFEYERVRDFIILHYHATQRDDSEFWRYCRNMEIPDTLRQK
                     IDLYRSHGRIFREGHELFADVAWLQVMHGQGIRANSYHPLADLPGEAQVEEYLASVKG
                     IVARCVDVMPDHSAYIAKHCASLSGR"
BASE COUNT          987 a         1602 c         1618 g          989 t
ORIGIN      
        1 ccgtcgcgaa cgacaagcac ctgggcgtgt ccatcagctt ttccgcggcc ccagccggcc
       61 tgcccggccc tccctcaccg ggagaactgg agcggatacg aaaagaaaac ggtgccattg
      121 caaaactcga ggtcctcgac ggcaacgtcg gctacatgcg agtgaacggt gctcctgctc
      181 tcgagggttc tcgtcccgcg gtcaccgccg cattcgcctt cctgcacgac actgacgccc
      241 tgattatcga caatcgcggc aacggcggcg gcgatcccaa taccgctgcg ttttatgaga
      301 gctatttgag cgaaggtaag ccccacctcg ctggcagctt tcactttcgc gaagggaacc
      361 gggtacagga gttctggacg acggatctgg gagagcactc gtatggcgcg cgcaaacctg
      421 ttttcgtgct cacttcagtc cggacgttct ctgctggaga gggaatcgcg tacaacctgc
      481 aggcgctcaa gcgcgcggtg atcgtgggtg agaagacggc cggcggcgcg acatcgccat
      541 tgcgcatgcc gatgggcaat caattcgtat tggctgtgcc cgtcgccacc ggcgtgaatc
      601 caattaccag gacgagctgg gaaggagtgg gtgtgattcc cgatgtgccg gtcgaggaag
      661 ccatcgcgct gagcaaggca cacacgctgg cgattgaacg gctacgggct tctgtcgcca
      721 atgatcgcga acggtccgcc ttcgactcca tcttgatgaa gattcagtcg atcgaagagg
      781 ctggatccgc aaagactatt cgtctgccgg ccacggatct ggtgggcgcg tatgttcccg
      841 tagtcggcac cggtacgacg gtcgtgatat ttgaaagaga cggccagctc acatggcacg
      901 tcgatggagc gcaggatcgc gcgctccttc atctgggtgg caatcgatat caacgcgagg
      961 gtctcccaag cggctacacc atcagttttc gcaagcaatc agggaaaact gaactactga
     1021 tggaagatcc cattcgccct tcggtgattc gggcgaagag caagagttga aggggcgcac
     1081 ccggagagat tccaactccc gaccacctgg ttcgtagttg gagagggtga aaagctcagg
     1141 caaggcgagg aacgtagata gttggtccga gcactatgta cgtcactgat ttgactcgac
     1201 gttgcacatg ggttaacacc cgcgatcaaa caagggttcg accacctcga catgaattca
     1261 cacgatgctg agcagcagga actggcacgt gccatcgggc tgttgggtag caacacccgt
     1321 catgctcggc tgctggcgta cgtgggtgcc cggtacttcc agggtcagga ggctgagctc
     1381 accgagttca agattgccac cgaggtattt ggcagatccg cgcagcgctt cgatgccaca
     1441 gaggacgcgg tggttcgcgt cgaagtgcac cggctgcgca agaagcttcg ggagatctac
     1501 gagaaggaca cccgtacgca ggggttgcag atctccctgc cgcccggcac ctatgttccg
     1561 agtttcacgc ccttgtccgc ccctccgccg gcgtccgacg caccggtatc cgagccggcc
     1621 agtcgatggc gtcgcattcc aaaatggacc atcctgaccg tcgtcatagt gagcgccgcg
     1681 atcgcgagcc tcatgattcg cgatggctcg cagcccgggc agactgccgt gccaggcgct
     1741 gcgccagcgc aaacgcagtc gcccgaacgc gcggcgccag gcgcgatctc cgagcttcac
     1801 attctcgcgg gataccacgg cagcgaggtc atagacaact cgggtgtccg ctggactccc
     1861 gatcgctact tcgcatcggg cggccagtgg tcgcgcgact tcgggctagt gcgcggaacc
     1921 agccggcctt tcctgttcgc gaactggcga accggcgaat tcggctacga catcccggtg
     1981 aagcccggcg tctacgagct gcggctattc ttcgtttcgc cctacaggat aggcgaggaa
     2041 aagctcggtg gattccaggt ggctctgcat ggcaagccgt tgctccagga ttacgacatc
     2101 aacgagagtg ccaacggcac cgatatcgcc gacgaactgg tgttcaggga cgtagcgccc
     2161 ggcgaagacg gattcatccg gctgtggttc tccaaccatg tagcgtcccc gctgttgaac
     2221 gcgctggaac tcgtgccggg tacgccgggc aaactccatc cgatccggat tctcacgcag
     2281 cccacctcgt tcgtcgacca caaaggtcag cgatggcggg ccgacgacta ctatctcaac
     2341 ggcttccgtt ccaccgaacg gcgcaaggtg agtggcaccg aggatcccga gttgttcggc
     2401 gcggaacgct tcggtcattt cagctacgcc attcccgtcg atcgtcgcgg gcgctacacg
     2461 gtgatcctgc actttgccga gctgtactac ggggcgcagc tcgcgggagc cggtggcgtc
     2521 ggcagccgtg tgttccatgt gttctgcaat ggccagacgc tgctgcgcga tttcgacatc
     2581 tacaaggaag caggcagcct gcgggtagtg accaagactt tcacgaacat cgttccctcc
     2641 gcgctgggaa agatcaatct ggatttcgag ccggtcgtga acaacgcgac ggtgtccgcc
     2701 atcgaagtga tcgaagagtc tccctgacgt tgaaggcatt ggtcaggtgc caagcgagat
     2761 cctgaatgca ctggcgtgac gcgtcggcaa gcgatccgga agttcgatga tcagcgcagc
     2821 atccgtctgc tcgaacttca gggatgtcct gacaccgagc agatgaacgc tgcgcacggc
     2881 gcgtttttcg tggcccgcgg agcggcctaa cgaagtaatg gcgacgcgac cccggggctc
     2941 gcccagcgtg atggcgtaca gcatcttgcc cttggtggtg aaacggatgt cctcggcctt
     3001 gtacccggcg ttctccttga attccccggc cgccgtctcc gtcggccctt cgccgtgcac
     3061 ggcccagggg cgggttccgt ggatcgcctc cgcgttcacg gccatccagg gcgcgagctc
     3121 cgtgagcagc gtttgcgatt ccggcggcag gcttccgtct ggatagagcg ccacgttcag
     3181 gagcaggttg ccgttcttgc tgacgatgtc cgccagcatg cggatcacgt cggaggcggt
     3241 cttgtattca tcgccatcct tgaaatacca gtcgccattc gaggtatcgg tctgccatgg
     3301 ccgcggattg atgccgctca tgacgccgcg ctcgacatcc tgtacgcagg cttcgggata
     3361 aaactcgcct gatccgagcg ccttgcagtt gtagaccaca tcgacacgcc cgcgtttggc
     3421 ggcgctgctg ttgtagaagt gagcgagcag cgaacgcccc acttcgccaa agggcatgcc
     3481 accgtccgag tagagcaggt ccggcgcgta ggagtcgacc aggtcgcgga tccggttgaa
     3541 ccagacctga tgaaacgcgg cgttggtcgt gtaccaggtt tgcgcgttgc ccttgtacac
     3601 ctcgtcgtgc ctggggtgat agagatccgc gtgggccggg ttcgcgccgt cgtagtcgac
     3661 gccgaacttc ggccagaact gatcgtattg atgactcggg taccaccagt tgtggctggc
     3721 gccgagatgc tcggatacac cgaagcgcag gccgcgacgt cgcgccgcat cgcgccacgc
     3781 gccgacgatg tcgcgcctgg gccccatggc caacgcgttc cagcgatggt ggcgcgaatt
     3841 ccacagatcg aagttgtcgt gatgcacggc catgctgacg aaatacttcg cgcccgcagc
     3901 gacgtaccga tccatgagcg cctctggatc gaacttctcc gctttccaaa gcggaatgat
     3961 gtccttgtag ccgaactcgg atggatgacc atagtgtttc aggtgatgcg cgtagtgcgg
     4021 gtggcctggc acatacatcc agcgcgcgta ccagtccccc gagcggggac cggcttgcgg
     4081 gccccagtgc gcccagatgc cgaacgtggc gtcgcgaaac caatccgggg cctggtaggt
     4141 cttgagagag tccgcggtgg gctggaacgg cccgcgggca accggcagct gcgacgctgc
     4201 cgcgcgcgcg accgcgcttg cggccagcgc ggcgtatagc ggtgtgccta acaatgatcg
     4261 ccgactgatc ctggtcatga ctttctccta gcgacctgac aaggacgcgc aatgctttgc
     4321 gatgtacgcc gagtgatccg gcatcacgtc cacgcagcgc gcgacgatcc ccttgacgct
     4381 ggcgagatac tcctccacct gggcctcgcc cggaagatcc gccagcgggt gataggagtt
     4441 cgctcgtatt ccctgcccgt gcatgacttg cagccacgcc acgtccgcga acagctcatg
     4501 gccctcgcgg aatatccggc cgtggctgcg gtacaggtct atcttctggc gcagcgtgtc
     4561 gggaatctcc atgttccggc aataacgcca gaactccgaa tcgtcacgct gcgtggcgtg
     4621 gtagtgcagg atgatgaaat cgcgcacgcg ctcgtattcg aactgcgctt cgcgattgaa
     4681 ctcggcaata tccggagtgc tgaacctcag atccggaaaa aacaccatga gccgcgcaat
     4741 catggtctgg atcaggtgga tgctggtcga ctccagtggc tcgatgaagc cgctggcgag
     4801 gcctaccgcg atacagttcc tgttccacgc cagtcgccgg cggcccgtga cgaacttgat
     4861 cgtgcggggt tcggcggtgg gtcggccgtc gaggttcgcg agcagtaccg ccgtcgcctc
     4921 gtcctcgctc atgtgcgcgc tgctgaacac atggccgttg ccggtgcgat gttgcaacgg
     4981 gatgcgccat tgccagccgg cacgatgcgc cgtcgatcgg gtatagggca gcagcgggct
     5041 cacggattcg cagggaaccg cgaccgcacg atcacagggc agccaatgcg tccactcttc
     5101 gtagcccgtg ccaagcgcct gctcgatcaa gagaccgcga aagcccgaac aatcgaggaa
     5161 caactcccct tccacctgcg cgccgctcgc gagctt
//
LOCUS       DATDEU010000651         3303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_272888, whole genome shotgun sequence.
ACCESSION   DATDEU010000651 DATDEU010000000
VERSION     DATDEU010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272888"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VIV63_15515"
     CDS             <1..260
                     /locus_tag="VIV63_15515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY6126061.1"
                     /translation="HWDYIYEPNAEEILDGLLMRYIESQVYRAAVENVASEMAARMVA
                     MKSASDNAGKLIGELQLIYNKARQAAITKELAEIVGGAAAV"
     gene            276..1697
                     /gene="atpD"
                     /locus_tag="VIV63_15520"
     CDS             276..1697
                     /gene="atpD"
                     /locus_tag="VIV63_15520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY6126062.1"
                     /translation="MATGKITQIIGAVIDVEFPRESIPKIYEALKLKDVDLTLEVQQQ
                     LGDGVVRTIAMGASEGLKRGIAVESTGKPINVPVGQATLGRIMDVLGAPQDERGPVQA
                     KEYLPIHRPAPSFSEQTGGQEMLSTGIKVMDLLMPFAKGGKIGLFGGAGVGKTVNMLE
                     LINNIAKQYSGLSVFAGVGERTREGNDFYHEMIESGVIDPKELSNSKVAMVYGQMNEP
                     PGNRLRVALSGLTMAEYFRDEVQGSSGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPS
                     AVGYQPTLAEEMGVLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDATIVL
                     SRQIAALGIYPAVDPLDSTSRMLDPLVVGDEHYNTARGVQQVLQRYKELQDIIAILGM
                     DELSQEDRQTVFRARKIQRFLSQPFNVAKVFTGQDGKIVPLAETIRGFKGILNGDYDH
                     LPEQAFYMVGGIDEAVEKAKTLN"
     gene            1707..2129
                     /locus_tag="VIV63_15525"
     CDS             1707..2129
                     /locus_tag="VIV63_15525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007969909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY6126063.1"
                     /translation="MANKTIHVDIVSAEGEIFAGEAEMVFVPASEGELGITPRHAPLL
                     TLIKAGEVRVKTAEGETSVFVGGGALEVQPRKVTILADTAVRAHDIDEAAALAAKTRA
                     EEALASSEDKIDQAHALAELAQAAAQLKLLEKLRKTRG"
     gene            2211..2978
                     /locus_tag="VIV63_15530"
     CDS             2211..2978
                     /locus_tag="VIV63_15530"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEY6126064.1"
                     /translation="MLLRTVDARRYVTPLREGGSLPAIVEGDDAGLYVLKFRGAGQGP
                     KALIAELIGGEIARALALEVPEIVFMTMDPVMARTEPDSEIQALIRASEGLNLASDYL
                     PGSVTYDPVAWPVDPELASRIVWFDALVTNVDRTAKNVNMLVWHRKLRLIDHGAALYM
                     HHDWGTALARSRDPFERIRDHVLLPCASRIDAVDAELAAKLTPELLQEVIAAIPTVWL
                     ADAGGEPRRADYLQFLTQRLEAPRAFAQEAQRAHARL"
     gene            2959..>3303
                     /locus_tag="VIV63_15535"
     CDS             2959..>3303
                     /locus_tag="VIV63_15535"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEY6126065.1"
                     /translation="MPTPGYDYAVIRVVPRVEREEFVNVGVILSCPSLDYLDARLHVD
                     HARLAALDPRVDFDLVEQHLRVIPTVCRGGAAAGAIGALTTRQRFHWLVAPRSTIIQM
                     SAVHSGFCGDDPS"
BASE COUNT          617 a         1094 c         1051 g          541 t
ORIGIN      
        1 agcactggga ttacatctac gagccgaatg ccgaggagat cctcgacggc ctgctgatgc
       61 gctacatcga atcgcaggtt tatcgcgccg cggtcgagaa cgtggccagc gaaatggccg
      121 cgcgcatggt cgccatgaag tcggcctcgg acaacgccgg caagctcatc ggcgagctcc
      181 agctcattta caacaaggcc cgccaggccg cgatcaccaa ggaattggct gagatcgtcg
      241 gcggcgcggc agcggtttag ttagaggatt tgcacatggc cacaggcaag attacccaga
      301 tcatcggcgc cgtcatcgac gtggaattcc cgcgcgagtc gattccgaaa atctacgaag
      361 cgctcaagct caaggacgtg gacctcacgc tcgaagtgca gcagcagctc ggcgacggcg
      421 tcgtgcgcac catcgccatg ggtgcctcgg aaggcctgaa gcgcggtatc gccgtggaat
      481 ccacgggcaa gccgatcaac gtgccggtcg gtcaggcgac gctcggccgc atcatggacg
      541 tgctcggtgc gccgcaggac gaaaggggtc ccgtgcaggc gaaggagtac ctgccgatcc
      601 accgccccgc gccgtcgttt tcggaacaga cgggtggcca ggaaatgctg tccaccggca
      661 tcaaggtcat ggacctgctc atgccgttcg cgaagggcgg caagatcggc ctgttcggcg
      721 gcgccggcgt cggcaagacc gtgaacatgc tcgagctcat caacaacatc gccaagcagt
      781 actcgggcct gtccgtgttc gcgggcgtgg gtgagcgtac ccgcgaaggc aacgacttct
      841 accacgagat gatcgagtcc ggcgtcatcg acccgaagga actctcgaac tcgaaggtag
      901 ccatggtcta cggccagatg aacgagccgc cgggcaaccg cctgcgcgtc gcgctctcgg
      961 gactgaccat ggccgagtac ttccgtgacg aggtgcaggg cagcagcggc aagggccgtg
     1021 acgtgttgtt cttcgtcgac aacatctacc gctacacgct ggccggcacc gaagtgtcgg
     1081 cgctgctcgg ccgcatgccg tcggccgtgg gttaccagcc gacgctggcc gaggaaatgg
     1141 gcgttctgca ggaacgcatc acttccacca agaccggttc gatcacctcc atccaggccg
     1201 tctacgtgcc ggcggacgac ttgaccgacc cgtcacccgc cacgaccttc gcccacctgg
     1261 acgccaccat cgtgttgtcg cgccagatcg cagcgctcgg catctacccg gccgtggatc
     1321 ccctggattc caccagccgc atgctggacc cgctggtcgt cggtgatgag cactacaaca
     1381 ccgcgcgcgg cgtgcagcag gttctgcagc gatacaagga actgcaggac atcatcgcca
     1441 tcctgggcat ggacgagctc agccaggaag accggcagac ggtgttccgc gcccgcaaga
     1501 tccagcgctt cctgtcgcag ccgttcaacg tcgcgaaggt gttcaccggc caggacggca
     1561 agatcgtccc gctggccgag accatccgcg gcttcaaggg catcctgaac ggtgattacg
     1621 atcacctccc ggaacaggcc ttctacatgg tcggcggcat cgacgaagcc gtcgaaaaag
     1681 ccaagacgct gaactaaggc acgcacatgg ccaacaagac catccacgtc gacatcgtca
     1741 gcgccgaagg cgagatcttc gctggcgagg cggagatggt gttcgtgccg gcatcggaag
     1801 gcgagctcgg catcacgccg cgccacgcgc cgctgctgac gttgatcaag gccggtgaag
     1861 tgcgtgtgaa gaccgccgaa ggcgagactt cggtattcgt cggtggtggc gcgctggaag
     1921 tgcagccgcg caaggtcacg atcctcgcgg atacggccgt gcgcgcgcat gacatcgacg
     1981 aagctgctgc cctcgctgcc aagacccgtg ccgaagaggc actggccagc agcgaagaca
     2041 agatcgacca ggcgcatgcg ctggctgaac ttgcgcaggc tgccgcacag ctcaagctgc
     2101 tcgagaagct gcgcaagacc cgcggctgat tcatccggag cccgccgcct cgcgggctat
     2161 cctgatatgc ggagctgacg cggcgccccg ggccgtgcat catgccgccc atgctcctcc
     2221 gcaccgtcga tgcccgccgt tacgtcacgc cattgcgcga aggcggctcg ctgcccgcca
     2281 tcgtcgaagg cgatgatgcg ggcctctatg tactcaagtt ccgcggcgcc ggccaggggc
     2341 cgaaggcgct gatcgccgag ctgatcggcg gagaaatcgc gcgcgcgctc gcgctcgaag
     2401 tgccagagat cgtgttcatg accatggatc cggtcatggc gcgcaccgag cccgactccg
     2461 agattcaggc actgatccgc gccagcgagg gactgaacct cgccagtgac tacctgcccg
     2521 gctcggtgac ctacgatccc gtcgcctggc ccgtggaccc ggaacttgca tcgcgcatcg
     2581 tctggttcga cgccctcgtc acgaacgtgg atcgcaccgc caagaacgtg aacatgctgg
     2641 tgtggcaccg caagctgcgc ctcatcgacc atggcgcggc gctgtacatg caccatgact
     2701 ggggcactgc actggcccgc agtcgcgatc ctttcgagcg tatccgcgac cacgtgctgc
     2761 tgccctgcgc cagccgcatc gacgccgtgg atgccgagct cgcggcaaag ctgactcccg
     2821 aactgctgca ggaagtcatc gccgcaatcc ccaccgtgtg gctggcggat gcaggcggcg
     2881 aacctcgccg cgctgactac ctgcagttcc tgacgcagag actcgaggcg ccgcgcgcct
     2941 ttgcacagga ggcgcaacgt gcccacgccc ggctatgact atgcggtgat ccgcgtggtg
     3001 ccgcgcgtcg agcgcgaaga gttcgtcaat gtgggcgtga tcctctcgtg tccgtcactg
     3061 gactatcttg atgcgcgcct gcatgtggac catgcgcggc tcgccgcgct ggatccacgc
     3121 gtggacttcg atctggtgga acagcatctg cgcgtgattc cgacggtctg ccgcggcggg
     3181 gctgctgcgg gcgccatcgg cgcactaacc acgcggcagc gctttcactg gctggtggcg
     3241 ccgcgcagca ccatcatcca gatgtccgcc gtgcacagcg gcttctgtgg tgatgatccg
     3301 tca
//
LOCUS       DATDEU010000652         3476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_858218, whole genome shotgun sequence.
ACCESSION   DATDEU010000652 DATDEU010000000
VERSION     DATDEU010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858218"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VIV63_15540"
     CDS             <1..655
                     /locus_tag="VIV63_15540"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6126066.1"
                     /translation="KRFDFGGGAAIYLMQPGAATRVRTWTPKAGHFFGFLITHGEAIS
                     IADYLTVRQSSEAVYRPTVHYSYHPSDSAIVSLHEFAGRNWHLQDRQRILLDEITGGI
                     DELGVLLAGHKKNAYWFGSQLSIDEARKLAPHNNATSLQVTVSCLSGMIWAMENPNAG
                     LVEPDEIDYVRNLEICMPYLGTVAGTYSDWTPLHDRERLFPEDLDKTDPWQFKNVRV"
     gene            complement(689..1045)
                     /locus_tag="VIV63_15545"
     CDS             complement(689..1045)
                     /locus_tag="VIV63_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126067.1"
                     /translation="MSDFFPLVTALVISFTLSTATVFAIYHPLHRLLEAVCPLGFTAA
                     FWTRAAVTLIYLFPLWVVLVFGLPDLQRAEFIPAGEIARRSLAAASFALVGIVVATGL
                     KLASVPAASNYDAPVR"
     gene            complement(1042..2892)
                     /locus_tag="VIV63_15550"
     CDS             complement(1042..2892)
                     /locus_tag="VIV63_15550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEY6126068.1"
                     /translation="MTLVFSLLTIQTLLGALDTVWNHEMAEGLPARRSARLELALHAT
                     REFIYAFVFLALAWREWHGAWATLIAAALLLEVGVTIADFVVEDRTRRLPAFERVLHT
                     VLTLLFGVVLMAMAPVLIGWWNEPTALMPIDHGVFSILLSVFAVGMTAWGIRDGVAAL
                     SHFRPAEWLRNPIEAATQPSGRGVLITGATGFIGGHVVRALRKRGEAVGVWTRDAARA
                     LVRFGPHVHVVTALADIPAGARIDSIVNLAGTPVIGLPWTRARRQLLIDSRVKTTQAV
                     LDWCATRTQPPRVLISASAIGFYGPAGDEWLTEDSPATAVFQSRLCVEREEAANAAEA
                     LGIRAVNLRIGLVLGSDGGIFPQLARPARLGMATRIGDGRQWMSWIHLFDLLRIIELA
                     LDEPSLRGAINAVAPAPERQGDFQRALARSLRRPYFMRVPARALRIALGEMAELLVDG
                     QRVAPRRLLNAGFEFRHYTLASALRDLVPDPSTPPAVRAADSNCDVWFNGDCPVCSRE
                     IGGYAKLVERRDLPVRFHDSMRVALPLSAYGLRREHLERRLYLRDENGRIQSGFRAVL
                     ALWARLPGYRRLVGVFSWPPLLALCETLYDHIVAPGLSAWARVRQSGART"
     gene            complement(2889..>3476)
                     /locus_tag="VIV63_15555"
     CDS             complement(2889..>3476)
                     /locus_tag="VIV63_15555"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEY6126069.1"
                     /translation="IGTDAWNRLPEAVHERFADTCDAVTYAGAFEIVRASALGRVFAW
                     LGTLVGTPVAPRAEDNVEARVRVRPDGDGVAWDREYRWSDGTHHVVRSTKVVADGKLI
                     EKLPARLCMPLDTYEDGGVLHFVSKGYYFDLGLGDFGRGLKLWLPGVLSPGVTHVEHV
                     DLGHGWFRFTMTVMHPLFGEIFFQTGRFSATEELP"
BASE COUNT          650 a         1157 c         1160 g          509 t
ORIGIN      
        1 caagcgcttc gatttcggcg gtggcgcggc tatctacctg atgcagccgg gcgccgccac
       61 gcgggtgcgc acctggacgc cgaaggcggg tcacttcttc ggcttcctga tcacgcacgg
      121 cgaagccatt tccatcgccg actacctgac ggtgcggcag agctccgagg ccgtgtatcg
      181 gccgaccgtg cactattcct atcacccgag cgattcggcc atcgtgtcgc tgcatgaatt
      241 cgccggccgc aactggcacc tgcaggaccg ccagcgcatc ctgctcgacg aaatcaccgg
      301 cggcatcgat gaactcggtg tgctgctcgc cggccacaag aagaacgcgt actggttcgg
      361 ctcgcagctt tccatcgacg aggcgcgcaa gctggctccg cacaacaacg ccaccagcct
      421 gcaggtcacg gtgtcgtgcc tgtcgggaat gatctgggcc atggagaacc cgaacgcggg
      481 tctcgtcgag cctgacgaga tcgactacgt gcgcaacctc gagatctgca tgccgtatct
      541 cggcacggtc gccggtacgt actccgactg gacgccgctg cacgatcgcg agcggctgtt
      601 cccggaagac ctcgacaaga ccgacccgtg gcagttcaag aacgtgcgag tctgatctct
      661 tcggcgtacc ccgtccgccg gacgtcagtc atcggacggg ggcgtcgtag ttagacgcag
      721 cgggcacgct cgcgagcttg aggccggtcg ccacgacgat tccgaccagt gcaaaggacg
      781 cggccgcgag cgagcggcgc gcgatctcgc ctgcggggat gaattccgcg cgctgcaggt
      841 cgggtaaccc gaacaccagc accacccaca gcgggaacag gtagatcaac gtgacggccg
      901 cgcgcgtcca gaacgccgcg gtgaatccca gcgggcagac ggcctcgagc agtctgtgca
      961 gcgggtgata gatcgcgaag accgtggccg tgctgagagt gaaggaaatc acgagggcgg
     1021 tgacgagggg gaagaagtcg ctcatgtccg ggctcctgac tggcggacgc gcgcccatgc
     1081 gctgagtcct ggtgcaacga tatggtcgta aagggtctcg cagagcgcga gcagcggcgg
     1141 ccaggagaac accccgacga gccgccggta tccgggcagt cgtgcccaga gcgccagcac
     1201 cgcgcggaag ccgctctgga tgcggccgtt ctcgtcgcgc agatagaggc ggcgctccag
     1261 gtgctcgcgg cgcaagccat aggcggacaa cggcagtgcc acgcgcatcg aatcgtggaa
     1321 tcgcaccggg aggtcgcgac gttcgacgag tttcgcgtag ccgccgatct cgcgggaaca
     1381 caccgggcaa tcgccgttga accacacgtc gcaattgctg tccgccgcgc gtactgccgg
     1441 cggcgtggac gggtccggaa cgagatcgcg cagggcgctc gccagcgtgt aatgacggaa
     1501 ttcgaaaccg gcattcagca gacgccgcgg cgccacgcgt tgtccgtcca ctaacaactc
     1561 cgccatctcg ccgagcgcga tgcgcaacgc gcgcgcgggg acgcgcatga agtaaggacg
     1621 gcgcaggctc cgtgccagcg cgcgctggaa gtcaccctgg cgttccggcg ccggcgccac
     1681 cgcgttgatc gcgccacgca gggaaggttc gtcgagcgcc agttcgatga tgcgcagcag
     1741 gtcgaagagg tggatccagg acatccactg acgcccatca ccgattctcg tggccatgcc
     1801 gagcctggcc gggcgcgcaa gctgcggaaa gattccgccg tcgctgccca gcaccaggcc
     1861 gatgcgcaaa ttcacggcgc gaatgccgag cgcctcagcg gcgttcgccg cttcttcgcg
     1921 ctccacgcac agccgggact ggaacaccgc cgtcgcggga gagtcttcgg tgagccattc
     1981 gtctccggcc ggaccataga agccgattgc gctcgcgctg atcaacacgc gcggcggctg
     2041 cgtgcgcgtc gcgcaccagt cgagcaccgc ctgggtggtt ttcacgcggc tgtcgatcag
     2101 cagctggcgg cgtgcgcgtg tccagggaag cccgatgacc ggcgtgcccg ccagattgac
     2161 gatggagtcg atgcgggcgc cggcaggaat gtccgcaaga gccgtgacga catgcacatg
     2221 cggcccgaaa cgcaccagcg cgcgggcggc atcgcgggtc cacaccccca cggcctcgcc
     2281 gcgtttgcgc agcgcgcgca cgacgtggcc gccgatgaag ccggtggcgc ccgtgatgag
     2341 cacgccgcgg ccggaaggct gtgtcgcggc ctcgatggga ttgcgcagcc attccgccgg
     2401 ccggaaatgg gacagtgccg cgacgccatc gcgaatgccc caggcggtca tgccgacggc
     2461 aaacacggaa agcagaatgg agaacacgcc atgatcgatg ggcatcaacg ccgtcggctc
     2521 gttccaccat ccgatcagca ccggcgccat ggccatgagc accacgccaa acaacaacgt
     2581 gagcacggtg tgcaacacgc gctcgaaggc gggcaggcgg cgcgtgcgat cttcgaccac
     2641 gaagtcggcg atggtgacgc ccacttcgag cagcagcgcc gcggcgatga gcgtcgccca
     2701 cgcgccgtgc cattcacgcc aggcgagggc caggaacacg aaggcataga tgaattcccg
     2761 cgtggcatgc agcgccagtt cgagacgggc ggaacgccgt gcgggcaggc cctcggccat
     2821 ctcgtggttc cagacggtat ccagcgcgcc taacaaggtc tggatggtga gcagtgaaaa
     2881 gaccagcgtc atggcaactc ctcggtggcg ctgaagcgcc cggtctggaa gaagatctcg
     2941 ccgaacagcg gatgcatgac cgtcatcgtg aagcggaacc agccgtggcc caggtcgacg
     3001 tgctcgacgt gggtgacgcc gggggacagg acgccgggaa gccacaattt caacccgcgg
     3061 ccgaagtcgc cgagtcccag gtcgaaatag tagcccttgc tcacgaagtg caggacgccg
     3121 ccgtcttcgt acgtatccag cggcatgcac agtcgcgccg gcagtttctc gatgagtttt
     3181 ccgtccgcca ccaccttcgt ggatctcacg acgtggtgcg taccgtcaga ccagcgatat
     3241 tcacgatccc aggcgacgcc gtcgccatcc gggcggaccc gcacgcgcgc ttcgacgttg
     3301 tcttcggcgc gcggcgccac cggcgtgccg acgagcgttc cgagccacgc gaatacgcgg
     3361 cccagcgcgc tcgcgcggac gatctcgaaa gcgccggcgt acgtcacggc atcgcaggta
     3421 tcggcgaagc gttcgtgcac cgcttccggc agacgattcc aggcatccgt tccaat
//
LOCUS       DATDEU010000653         8234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_272969, whole genome shotgun sequence.
ACCESSION   DATDEU010000653 DATDEU010000000
VERSION     DATDEU010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8234
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272969"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VIV63_15560"
     CDS             complement(<1..657)
                     /locus_tag="VIV63_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEY6126070.1"
                     /translation="MPNAPKSRNKPIGDAYWVIEDRLLAGRYPGGKTPKEAERRVQSL
                     LDAGFDAFIDLTEAGELPPYDLYLPGSALYVRKPIPDHGVPRFTEHMTEIQAEIDGAL
                     SRGRRIYVHCRAGIGRTGTVVACHLIEQGMAPDAALIRLNELWQGNDRAATWPDVPET
                     DEQRDYILAWTPAVDPTRAPAVMDAARTLRERFQGALLGLAVGDALAAHTQFRKPGTF
                     A"
     gene            complement(629..1468)
                     /gene="ttcA"
                     /locus_tag="VIV63_15565"
     CDS             complement(629..1468)
                     /gene="ttcA"
                     /locus_tag="VIV63_15565"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY6126071.1"
                     /translation="MNAEAQTVIENSPEWPSQTAKKLGAHLRGLVGKAIEDYRMIADG
                     DRVMVCLSGGKDSYTLLDVLLSLKRSAPIDFDLIAVNLDQKQPDFPEEVLPNYLKELG
                     VPYHVIEQDTYSVVKRVIPEGKTMCGLCSRMRRGALYRYAGENGITKIALGHHRDDIV
                     ETLFLNMFHGGRLKAMAPKLKSDDGRHIVIRPLAYVAESLIERYARARQFPIIPCKLC
                     GSQDNAQRKEVKRMLAEWERQYPGRTESIFSALRNVENAHLADPRTFDFPGLDAERAQ
                     IPE"
     gene            complement(1465..2235)
                     /locus_tag="VIV63_15570"
     CDS             complement(1465..2235)
                     /locus_tag="VIV63_15570"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HEY6126072.1"
                     /translation="MKSWRGGLIGAISAVFLLAAVLVPAMPQPLTYHAFADCRTLFGV
                     ANFLNVASNIPFLISGALGLALIWSGRVDFIDPREQLPYLVFFLGALLTCFGSAWYHA
                     QPDNARLVWDRLPMTLGFAGLVAAALAERMDLKLGLRSLWPLLAFGVITVFYWYATER
                     SGSGNLIPYAAYQAWAIVIIVVLLLAYPARRYTHGGLLAWAAGWYGLAKVFETFDLQI
                     YRLLGGTLSGHTIKHVLASFAVFAIVRQLRLRNRIPAA"
     gene            complement(2232..3755)
                     /locus_tag="VIV63_15575"
     CDS             complement(2232..3755)
                     /locus_tag="VIV63_15575"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEY6126073.1"
                     /translation="MAQTPYKQLEQEWKRLHAFRGAMAILRWDAAVMMPRGSADVRGE
                     QLAALETEYHSLVTAPRITRLLDRAQANTGGLVDWQISNLREMRRQRDFAIATPVALV
                     SRLAKAASKAEAKWLYARETGKFEDFAPHLEEVVHLVRDKAQLLGQARNLAPYDALVD
                     EFSPGLTSGEIDAMFKALARRLPGLIREVLARQEQRPILPIVGKFSAGKQRALAVEVM
                     KAVGFPFDRGRLDESEHPFTEGVPGDVRVTTRFDPTEPFSGLLGALHETGHAMYDVGL
                     PAEYSDQPVGRDRGMALEESQSLLMEMIVCRSRGFVSYLKPLLEKHFGVSGPEWSEEN
                     LYAHLTRVKRGPIRVDADELTYTLHIQLRYELENQLLSGELAVRNLRDAWNSSMEQRL
                     GVKPQNDLEGVLQDVHWAVGSFGYFPSYGLGAVMAAQFFEALRESVPDIESQIARGEF
                     AGLIGWLRENVHQHGARITTQELLKLSTGKPLSATAALRYLEAKYLGSESVVGSAAA"
     gene            complement(3946..7137)
                     /locus_tag="VIV63_15580"
     CDS             complement(3946..7137)
                     /locus_tag="VIV63_15580"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6126074.1"
                     /translation="MMISDLSVRRPVFATVMSLLLFILGIGAVTRLSLREFPDVDRPV
                     VSIETRYRGAGSDVVESRITQIIENEISGIEGVERLNSSSRDERSQINVEFTLDRDLD
                     SAANDVRERLSRVASRLPEEADPSQITKVDSGQDPIIFLNVASTQRNMLEITDFMERY
                     LVDQFATLEGVASVRMNGARRYAMRVWLSRENLAARQLTVADVENSLLRENVELPAGR
                     LESREREFSLRTDTNLRTEEDFRNLVVGRGPDGYLVRLGEVAEVRLASDDDRSLSRSN
                     GAIGLSMAVIPQSKANVLATSGAVKKRLAEIQPTLPKDMLVEVNIDNGVFIAASLKNV
                     VFALTETLILVLIVIFLFLGTLRATLIPAVTIPVSIVAAALVMAALDYSINTLTLLGA
                     VLAIGLVVDDAIVVLENIVRRIEGGEPPVLASISGSREIGFAVIATTLVLMAVFVPVS
                     FMSGDTGRLFGEFGVTVAAAVGFSAFVALSLTPMMASKLFANGMPKSRMVGNIDRVFH
                     NLDERYATGLRRAMTGRKPLWIVGGALGLFGVAIALFVVGIPFQGLRLPTELAPQEDR
                     GMVQIFITAPEGSSIQYLDRQLREVEQVAMEEVENGNARRVLTRSGGFGRSAEVNIGR
                     VMVPLNLWDERDESAGEITNRIRQRTQNISGARVNANQMGSPINRNSTRPLAIAMGGG
                     EYEEIVQWRDKVMQRVAAENPRILNLESDYLERQPRVVVQIDRNKAADLGVSLQTVGR
                     TLETMMGSRIVTTFERAGEEYDVVLQARDTQRASVGDLDNLYVRSDKNNELVPLSALV
                     RIDERAGPTELRRTDRMRAIELAGSLAPDYTLGEAVEYMERVIREEAPNAQLIYNGET
                     YQLKKSGDALWITFAFALLIVYLVLAAQFESFIHPIVILVTVPLALTGALLGIFLFNS
                     TINIFSQIGCIMLIGIACKNGILIVEFANQLRDRGVEFVDAIIQSSATRLRPVLMTSL
                     CTAFGAVPLMLAHGAGAESRESIGATVFFGTIVSLAMTLYVVPALYLLIARNTRSPEY
                     MTRLIDTLLGGRPTVRGTPATAESTAAPSPDRS"
     gene            complement(7134..>8234)
                     /locus_tag="VIV63_15585"
     CDS             complement(7134..>8234)
                     /locus_tag="VIV63_15585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6126075.1"
                     /translation="VCAAWAIYATQHRPTAAAGGPPSGGMRNNAPAAVMLAPVRAERI
                     SQKLEALGNARANESVDISTKISNIVTAVTFRDGERVRRGQVLVQLDNAQARADVAAA
                     QAAVTESESLYNRSRELLNTQALSKSSFDQLEATLKANRARLGAASARLEDTVIRAPF
                     SGRVGLRRVSVGTLISPGDVITTLDDTSVIKLDFSVPENFLASLREGLSIRASAPAFP
                     GRSFAGSVSSIDSRVDIATRSVTVRALLANEDGALKPGMFLNVSLANDEREALVIPEE
                     ALTPEAERQFVFVVADGKATRREVKIGGRQPGTVEIVAGLTAGERVVVEGTQKIRDGA
                     SVVASDRIVDSLPDQNEGQSHEASASPARIAQ"
BASE COUNT         1437 a         2616 c         2761 g         1420 t
ORIGIN      
        1 ggcgaacgtg cctggttttc gaaactgcgt gtgcgcggcg agcgcatcac ccaccgcgag
       61 acccagcaat gcgccctgga accgttcgcg caacgtgcgc gccgcatcca tgaccgcggg
      121 tgcgcgcgtg ggatccacgg ccggcgtcca ggccaggatg tagtcgcgct gctcgtcggt
      181 ctcgggcacg tcgggccagg tcgcggcgcg atcgttgccc tgccagagtt cgttgaggcg
      241 gatcagcgcg gcatcgggcg ccatgccctg ctcgatgaga tggcaggcga ccacggtgcc
      301 ggtgcggccg attcccgcgc ggcaatgcac gtagatgcga cggccgcggg acagcgcccc
      361 gtcgatctcc gcctggatct ccgtcatgtg ctcggtgaag cgcggcacgc cgtgatccgg
      421 aatgggcttg cgcacgtaca acgcgctgcc gggtagatag agatcgtagg gcgggagttc
      481 gcccgcctcg gtcaggtcga tgaaggcgtc gaagcccgcg tccagcagcg actgcacgcg
      541 ccgttcggct tccttgggcg tcttgccgcc cggatacctt ccggccagca ggcgatcctc
      601 gatcacccag taagcatcgc cgatgggttt attccgggat ttgggcgcgt tcggcatcca
      661 gccccggaaa atcgaaagtc cgcggatcgg ccaggtgagc gttctcgaca ttacgcaatg
      721 cggagaagat cgactcggtg cggccgggat attgccgctc ccactcggcc agcatgcgct
      781 tgacctcttt gcgctgcgcg ttgtcctggg acccgcacag cttgcaggga atgatcggga
      841 actggcgcgc gcgcgcgtat cgctcgatca gcgactcggc cacgtacgcc agcgggcgga
      901 tcacgatgtg ccgtccgtca tcggacttga gcttcggcgc catggccttg agccggccgc
      961 cgtggaacat gttgaggaac agcgtctcga cgatgtcgtc ccggtggtgc cccagtgcga
     1021 tcttggtgat tccgttttcg cccgcgtagc gatacaacgc gccacggcgc atgcgcgagc
     1081 acagcccgca catcgtcttg ccttccggga tcacgcgctt gaccacgctg taggtgtcct
     1141 gctcgatcac gtgatacggg acaccgagct ccttcaggta gttgggcagc acctcttccg
     1201 ggaaatccgg ctgcttctgg tcgaggttga cggcaatcag gtcgaagtcg atgggcgcgg
     1261 accgcttgag cgaaagcagc acgtctaaca aggtgtagga atccttgccg cccgacaggc
     1321 acaccatcac gcgatcgccg tcggcgatca tgcggtaatc ctcgatggcc ttgcccacga
     1381 ggccacgcag atgcgcgccg agcttcttcg cggtctgcga aggccactcg ggcgaattct
     1441 cgatgacggt ttgagcctcg gcgttcatgc ggccggaatt ctattccgca ggcgcagttg
     1501 gcgcacgatg gcgaacacgg cgaacgacgc cagcacgtgt ttgatggtgt ggccgctcag
     1561 cgtgccgccc aggagccggt agatctgcag gtcgaaggtc tcgaacacct tggcgagccc
     1621 ataccagccg gcggcccagg cgagcagccc gccgtgggtg taacggcgag ccgggtaagc
     1681 cagcagcagc accacgatga tgacgatggc ccaggcctgg taggcggcgt acggaatcag
     1741 gttgccggac cccgaccttt cggtggcata ccaatagaaa accgtgatca cgccgaatgc
     1801 gagcagcggc cacagcgacc ggaggccgag cttcagatcc atacgctcgg ccagggccgc
     1861 cgccaccagt cccgcgaagc ctagcgtcat gggcagccgg tcccagacca ggcgcgcgtt
     1921 gtcgggttgc gcgtgatacc aggccgaacc gaaacaggtg agcaacgcgc cgagaaagaa
     1981 cacgaggtac gggagctgtt cgcgggggtc gatgaagtcc acgcgccccg accagatgag
     2041 cgccagcccg agggcgccac tgatcaggaa cgggatgttg gaagccacgt tcaggaagtt
     2101 ggcgacgccg aacagagtcc ggcagtcggc gaacgcgtgg tacgtcagcg gctgcggcat
     2161 cgccggcacc agcaccgcgg cgagcagaaa gacggcgctg atcgcgccga tcagcccacc
     2221 ccgccacgac ttcatgccgc ggcgctgccg actaccgatt cgctgccgag atacttggct
     2281 tcgaggtaac gcagcgcggc cgtggccgac agcggtttac cggtggaaag tttgaggagt
     2341 tcctgcgtgg tgatgcgagc gccgtgttga tgcacgttct cgcgcagcca gccgatgagg
     2401 cccgcgaatt cgccccgcgc gatctgcgac tcgatgtcgg gcacggattc gcgcagcgcc
     2461 tcgaagaact gcgcggccat gaccgcaccc aggccgtacg aaggaaaata gccaaaggat
     2521 ccgacggccc agtgcacgtc ctgcaatacg ccctcgaggt cgttttgcgg cttcacgccg
     2581 agccgttgtt ccatgctgga attccacgcg tcgcgcaggt tgcgcaccgc gagctcaccg
     2641 gacaacagct ggttctcgag ctcgtagcga agctggatgt gcaacgtgta cgtgagctca
     2701 tcggcgtcga cgcggatcgg accgcgcttc acgcgcgtca ggtgcgcata gaggttttct
     2761 tcggaccact cgggcccgga cacgccgaag tgcttttcca gcagcggctt gaggtagctc
     2821 acgaagccgc ggctgcggca tacgatcatc tccatgagca gcgactggct ttcttccagc
     2881 gccatgccgc gatcgcggcc cacgggctga tcggaatatt ccgccggcag gccgacgtcg
     2941 tacatcgcgt gaccggtttc atgcagcgcg ccgagcaggc ccgagaaagg ctcggtcgga
     3001 tcgaagcgcg tggtgacacg cacgtcgccg ggaacgcctt cggtgaacgg gtgttcgctc
     3061 tcgtcgaggc ggccgcgatc gaacgggaaa ccgacggcct tcatgacttc cacggcgagc
     3121 gcgcgctgct tgccggcgct gaacttgccg acgatcggca ggatgggccg ctgttcctgg
     3181 cgcgcgagca cttcgcggat gagccccggc agccggcgcg cgagcgcctt gaacatggcg
     3241 tctatctcgc ccgaggtcag ccccggactg aattcgtcaa ccagcgcgtc atagggcgcg
     3301 aggttgcgcg cctgccccag caactgcgcc ttgtcgcgca cgaggtgaac cacctcttcg
     3361 agatgcggtg caaaatcttc aaactttccg gtctcgcgcg cgtacagcca tttcgcttcg
     3421 gccttcgatg ccgctttcgc gaggcgcgag accagcgcga cgggagttgc gatggcgaaa
     3481 tcgcgctgac ggcgcatctc gcgcaggttg gaaatctgcc agtccacgag accgccggtg
     3541 ttggcctggg cgcgatcgag aagtcgcgtg atccggggcg cggtgaccag cgagtggtac
     3601 tcggtttcga gcgcggcgag ttgctcgcca cgtacgtcgg cactgccgcg cggcatcatg
     3661 accgcggcgt cccagcggag gattgccatc gcgccacgaa acgcatgcag gcgtttccat
     3721 tcctgctcga gttgcttgta gggggtctgg gccatgggga cgatcacgcg gggcgggcaa
     3781 actgacgggc gccagtctac cagcgaccct cgttccggcc tcccccacac gccatttatc
     3841 ctttcaggat catggactta agaagtcagg tttgggttga cagcctgggg ctgatccggc
     3901 cccgaaagac acatttatgt aacaaagcgc tgtggcgctt tcgtcttatg agcgatccgg
     3961 tgaaggcgcg gccgtggatt ccgctgttgc cggcgtgcca cgcacggtcg gacgcccgcc
     4021 gagcagcgtg tcgatgagcc gcgtcatgta ttccggcgag cgcgtgttgc gcgcgatgag
     4081 caggtacagc gcgggtacga cgtacagcgt catggccagg gagacgatcg tcccgaagaa
     4141 caccgtggcg ccgatcgatt cgcggctctc ggcgccggcg ccgtgcgcca gcatgagcgg
     4201 gaccgcgccg aacgcggtgc acaggctggt catgagcacg gggcgcaggc gggtcgccga
     4261 agactggatg atggcatcca cgaactcgac gccacgatcg cgaagctggt tggcgaactc
     4321 gacgatgagg atgccgttct tgcaggcgat gccgatcagc atgatgcagc cgatctggct
     4381 gaagatgttg atggtcgagt tgaacaggaa gatgccgagc agcgcgcccg tgagcgcgag
     4441 cgggaccgtc acgaggatca cgatggggtg gatgaaactc tcgaactgcg cggcgagcac
     4501 gaggtagacg atgagcagcg cgaacgcgaa ggtgatccac aacgcgtcgc cggatttctt
     4561 gagctgatac gtctcgccgt tgtagatgag ctgcgcgttc ggcgcttcct cgcggataac
     4621 gcgctccatg tattcgacgg cttcgccgag cgtgtagtcc ggcgcgagcg agccggccag
     4681 ctcgatcgcg cgcatgcgat ccgtgcggcg cagctcggtg gggcccgccc tttcgtcgat
     4741 gcgcaccagc gccgacagcg ggaccaactc gttgttcttg tcggagcgca cgtaaagatt
     4801 gtcgaggtcg cccaccgaag cccgctgcgt gtcccgagcc tgcaggacga cgtcgtattc
     4861 ctcgcccgcc cgttcgaagg tggtgacgat gcgcgacccc atcatggtct cgagcgtgcg
     4921 gccgacggtc tgcagcgaaa cgccgaggtc cgccgccttg ttgcggtcga tctgcacgac
     4981 gacgcgcggc tggcgctcga ggtagtcgga ttccaggttg aggatgcgcg gattctcggc
     5041 ggcgacacgc tgcatgacct tgtcgcgcca ctgcacgatt tcttcgtatt caccgccgcc
     5101 catcgcgatc gcgagcggac gggtgctgtt gcggttgatg ggactgccca tctggttggc
     5161 gttcacacgc gcgccggaaa tgttctgcgt gcgctggcgg atacggttgg tgatctcgcc
     5221 cgcgctctcg tcgcgctcgt cccacaggtt gagcggcacc atcacgcggc cgatgttcac
     5281 ctcggcgctg cgcccgaaac cgcccgatcg cgtgaggaca cggcgcgcat tgccgttctc
     5341 gacctcttcc atcgcgacct gctcgacctc gcgaagctgc cggtcgaggt actggatgct
     5401 ggaaccttcg ggcgccgtga tgaagatctg gaccatgccg cggtcttcct gcggcgcgag
     5461 ctcggtgggc aatcgcagcc cctggaaggg gatgccgacg acgaacaggg cgatcgccac
     5521 tccgaacagg cccaacgcac cgcccacgat ccagagcggc ttgcgtcccg tcatggcgcg
     5581 gcgcaggccc gtggcgtaac gctcgtcgag attgtggaac acgcggtcga tgttgccgac
     5641 catgcgcgat ttcggcatgc cgttcgcgaa cagtttcgag gccatcatgg gcgtgagcga
     5701 aagcgcgacg aatgcggaaa agcccacggc cgccgcgacg gtcacgccga attcgccgaa
     5761 caaccgtccg gtgtcacccg acatgaacga cacgggcacg aacaccgcca tgagcaccag
     5821 cgtggtggca atgacggcga acccgatctc gcgcgagccg ctgatggatg cgagcacggg
     5881 aggctcgcca ccctcgatgc gccgcacgat gttctcgagc acgacgatgg cgtcgtcgac
     5941 gacgaggccg atcgccagca ccgcgccgag caatgtgagt gtattgatcg aatagtcgag
     6001 cgccgccatg accagcgccg cggccacgat cgacaccggg atggtgacgg cgggaatgag
     6061 cgtcgcgcga agagtcccga ggaacaggaa gatgacgatg agcacgagga tcagcgtctc
     6121 ggtcagtgcg aacaccacgt tcttcagtga tgccgcgatg aacacgccgt tgtcgatgtt
     6181 gacctcgacg agcatgtcct tgggcagtgt gggctggatc tccgcgaggc gtttcttgac
     6241 cgcacccgag gtcgccagca cgttggcctt cgattgcgga atgaccgcca tcgacaggcc
     6301 gatggcgccg ttcgagcgcg agaggctgcg gtcgtcatcg gaggcgaggc gcacttcggc
     6361 cacttcgccc aggcgcacga ggtaaccatc aggcccacgg cccacgacga ggttgcggaa
     6421 gtcttcttcg gtgcgcaggt tggtatccgt gcgcaatgag aattcacgtt cgcgcgactc
     6481 gagccggccc gcgggcaatt ccacgttttc gcgcaacagc gagttctcga cgtcggcgac
     6541 ggtcagctgg cgcgcggcga gattctcgcg cgacagccac acacgcatgg cgtagcggcg
     6601 cgcgccgttc atgcgcaccg aggccacgcc ttcgagcgtc gcgaactggt cgacgagata
     6661 ccgttccatg aagtcggtga tctcgagcat gttccgctgc gtgctcgcga cgttcaggaa
     6721 gatgatcggg tcctggccgc tgtccacctt ggtgatctgc gacggatcgg cttcttcggg
     6781 aagccgcgag gcgacacgcg acaggcgctc gcggacgtcg ttcgccgcgc tgtcgaggtc
     6841 gcggtcgagc gtgaattcga cgttgatctg cgagcgctcg tcgcgactgg aggagttcag
     6901 gcgttcgacg ccctcgatgc cggaaatttc gttctcgatg atctgcgtga tgcgtgactc
     6961 gaccacgtcg gagcccgcgc cccgataacg cgtctcgatg ctcaccacgg gccggtcgac
     7021 gtcggggaat tcgcgcaacg acaggcgcgt gacggcgccg atgccgagga tgaacagcag
     7081 cagcgacatc accgtcgcga agacgggtcg ccgcaccgac aggtccgaga tcatcattga
     7141 gcgatgcgcg ccgggctggc gctggcctcg tgcgactggc cttcattctg atccggcagc
     7201 gaatcgacga tgcggtcgga ggccacgacg ctcgcgccat cgcgaatctt ctgcgtgccc
     7261 tcgaccacca cgcgttcacc ggcggtgaga cccgcgacga tttcgacggt gccgggctgg
     7321 cgtccgccga tcttcacttc gcggcgcgtc gccttgccat cggcaacgac gaatacgaat
     7381 tggcgctcgg cctcgggtgt cagcgcctct tccggaatga ccagcgcctc gcgttcgtcg
     7441 ttcgcgagcg agacgttgag gaacatgccg ggcttgagcg cgccatcttc attggccagc
     7501 aacgcgcgca ccgtgaccga acgcgtggcg atatcgacgc gcgagtcgat gctggaaacc
     7561 gagcccgcga aactgcgtcc ggggaacgcc ggcgcactgg cgcgaataga tagtccttcg
     7621 cgcagcgacg cgaggaagtt ttccggcacc gagaaatcga gtttgatgac gctggtgtca
     7681 tcgagcgtgg ttatgacgtc gcccgggctg atgagcgtgc cgacgctgac gcggcgcaag
     7741 ccgacgcgtc cggagaacgg cgcgcggatg accgtgtctt cgaggcgcgc cgatgcggcg
     7801 ccgagcctgg cgcgattcgc cttcagcgtc gcctcgagtt gatcgaagct cgacttcgag
     7861 agcgcctgcg tattcagcaa ctcgcggctg cggttgtaga ggctctcgct ttcggtaacc
     7921 gcggcttgcg cggcggccac atcggcacgc gcctgcgcat tgtcgagctg caccagtacc
     7981 tgcccgcgtc tgacgcgctc accatcgcga aacgtcaccg ccgtaacgat gttcgaaatc
     8041 ttggtgctga tgtcgacgga ttcattggcg cgcgcgttgc caagcgcctc gagcttctgg
     8101 ctgatgcgtt cggcgcgtac cggcgcaagc atgactgccg cgggtgcgtt gttgcgcatg
     8161 ccaccggacg gcggaccgcc cgccgcagcc gtgggtctgt gctgcgtcgc atagatagcc
     8221 caggcggcgc aaac
//
LOCUS       DATDEU010000654         5888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_858391, whole genome shotgun sequence.
ACCESSION   DATDEU010000654 DATDEU010000000
VERSION     DATDEU010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858391"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1312)
                     /locus_tag="VIV63_15590"
     CDS             complement(170..1312)
                     /locus_tag="VIV63_15590"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6126076.1"
                     /translation="MKRGLLSLLLSAWCVCAQADAALQESLSAVIANARDRGKIPAIA
                     AMVQINGKVEAQAVVGVRALRHRERATLQDRWHLGSDIKAMTATLIARLAEKGTLKLD
                     DTLAELLPAMAPAMDPAFRTVTMTQLLSHTAGVAPLVDPKELPPVLAVIAPYQEVKAQ
                     RAAIASHFLSRPPTSKPGAFVYSNVGYVIAGAVAEARTGRSFEELIRAEVWAPLGISH
                     AGFGAPGRSGKFDEPLGHEVGLFGLKAVDPGSRRSDNLPVFAPAGTAHMTLPDWMLFA
                     QDQLDGAHGHGKLLESATYRKLHVPVTPGYALGWRVAVAPDGTPAVLTHGGSNGMWKA
                     AIHITPANYRIVLVAMNAAGKPANDAADTIITELTGVNARARPRGK"
     gene            1387..1797
                     /locus_tag="VIV63_15595"
     CDS             1387..1797
                     /locus_tag="VIV63_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6126077.1"
                     /translation="MKTLALIVSLAAGTSFAAENAPPAKVTPVMTRDLEGIPGKEMLV
                     LVVEYPPGGVSAPHRHDASVFVYVLEGAVTMQVAGSAPVTLKAGGSFYETPGDVHVTS
                     ANASSSEPAKFLVYMLKNKGAPPSTPVTGVQPGG"
     gene            1805..2377
                     /locus_tag="VIV63_15600"
     CDS             1805..2377
                     /locus_tag="VIV63_15600"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY6126078.1"
                     /translation="MLPALLDQYGYLLIFVGTFVEGETLLMLGGYFAHRGYLDLAGVM
                     ATAFAAAVCGDQLFFHIGRRHATRLFERFPRLHDKVRVAVHRAERHQTLVVLGMRFLW
                     GLRIAMPLAMGMTRMDGRKFFWLNLISAAVWSVLFAMLGFAGSQLFTHLIDDLHRYEL
                     GIAGGLVGIGVVVLGIRWLGARRPEQMHDE"
     gene            2430..4526
                     /locus_tag="VIV63_15605"
     CDS             2430..4526
                     /locus_tag="VIV63_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6126079.1"
                     /translation="MSAKDPVFPAADDAQSRLAAVIEFSDDAIITKTLNGVITTWNPG
                     AARIFGFTAEEAIGQPVTMLIPENHLDEEPDILNRLRRGERIEHYETVRRRKDGTLIQ
                     VSLTVSPIKDATGRVIGASKIARDITRQKRAEALIREQADVLALLDTTGKAIGSKLDL
                     QNVLQTVTDTATHLTGAKFGAFFYNVINEHGEAFLLYTLSGASRESFEKFGLPRNTAV
                     FHPTFTGAGVVRSPDITQDPRYGKMSPHYGMPKGHLPVRSYLAVPVISRNGEVMGGLF
                     FGHPEVDQFSERAEQLVVGVASQAAVAMDNARLYEAAQREIASRERAEAALRETDQRK
                     DEFLATLAHELRNPLAPIRQATLISMSASATEDQKRWSHEVITRQVHHMSLLLDDLLD
                     ISRITRGTLELRTEMTDLGAVVDAAVETSRPAIEAKMHQFVIDLPREPVKFAADPLRL
                     AQVLSNLLTNAAKYTDPRGTIRLRASADADNVTITVADTGIGLSSDALSAVFTMFSQV
                     QSTRDRSDGGLGIGLALSKGLVQLHGGTIGASSPGPGRGSEFVVKIPRVRIASATTSD
                     SARAGEAPRRVRRVLIADDNRDAAESLAMLLEMQGHAITVVHDGRQALAAIEESPPEV
                     ALLDIGMPHIDGYEVARQVRSDTRRQNVLLIAVTGWGQEQDKARAMAAGFDLHFTKPI
                     EPQRLFDLLGAELPTR"
     gene            4542..>5888
                     /locus_tag="VIV63_15610"
     CDS             4542..>5888
                     /locus_tag="VIV63_15610"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY6126080.1"
                     /translation="MALSVFDLFKIGIGPSSSHTVGPMRAARQFALRLRDQRVASISV
                     MLYGSLGATGKGHGTDKAILLGLSGHEPDTVDVDAIAGIIERIRTTHELQLLGTHALP
                     FDERRDLTFNHKDTLPLHSNGMLFKAFAEDGSELTTGTYYSVGGGFVVSESESPGGGT
                     EKRIVPDLTVLPFPFRNGDELLALCEREKSSIASIMRRNERVWRTDAEIDQGLLHIWH
                     VMQACVKRGCETPGVLPGGYKVKRRAPDLYAQLTKPASAAPHDPLIALDWVNLWALAV
                     NEENAAGGRVVTAPTNGAAGIVPSLMHYYARFVHGANDAGIVDFLLTAAAIGFLYKEN
                     ASISGAEVGCQGEVGVACSMSAAALTAVLGGTVAQVENAAEIAMEHHLGLTCDPVGGL
                     VQIPCIERNAIGAAKAINVARMALRGDGTHYVSLDKVIKTMRETGADMKTKYKETSR"
BASE COUNT         1045 a         1923 c         1904 g         1016 t
ORIGIN      
        1 agcgaatcga cgttgctgcc gtcggtggcg aagacggcgc cggccgcgcc gagattcacg
       61 gctgcgaagg cggtcatcag gcgccagtgg tttggggcgg tcacgccgcg caagtgttca
      121 ccttcaggtt tgcgattgca aagtcccatg agtagtaaga gccgtctcac tacttgcccc
      181 taggccgtgc tcgtgcattc acccccgtca gttcggtgat gatggtgtcg gccgcgtcgt
      241 tcgcgggttt gccggccgca ttcatcgcga ccaggacgat acggtagttt gccggcgtga
      301 tgtggatagc ggccttccac atcccgttgc ttccgccgtg cgtgagcacc gccggcgtgc
      361 cgtccggagc cacggcgacg cgccacccga gcgcgtagcc cggcgtcacg ggcacatgca
      421 gttttcgata tgtcgcggac tcgagtagtt tgccgtggcc atgcgctcca tcgagctggt
      481 cctgcgcaaa cagcatccag tccggcagcg tcatgtgcgc ggtacccgcg ggcgcgaaca
      541 ccggcaggtt gtcgctccgt cgactgccgg ggtcgaccgc cttgagcccg aacagaccca
      601 cttcatgacc gagcggttca tcgaactttc cactgcggcc tggcgcgccg aatccagcgt
      661 ggctgatgcc gagcggtgcc cacacctcgg cacgaatcag ctcttcgaag cttcggcccg
      721 tgcgcgcttc ggcgaccgcg cccgcgatga catagcccac attggaatac acgaacgcac
      781 ccggcttcga cgtgggcggc cgtgacagga agtgactggc gatggcagcg cgctgggcct
      841 ttacctcttg ataaggagcg atgaccgcca gcacgggcgg caactccttc ggatccacga
      901 gcggcgcgac gcccgcggtg tggctcagca actgggtcat cgtcacggtg cggaaagccg
      961 gatccatggc cggcgccatc gcgggcagca gttccgcgag cgtgtcgtcg agtttcagcg
     1021 tgcccttctc ggccaggcgc gcgatcagcg tcgcggtcat cgccttgatg tccgagccca
     1081 gatgccagcg gtcctgcaac gtcgcccgct cgcgatgccg gagggcacgc acgcccacga
     1141 ccgcctgcgc ttcgaccttt ccgttgatct ggaccatcgc ggcgatcgcc gggatcttcc
     1201 cccggtcgcg cgcgtttgcg atgacggccg acagggactc ctgcagggcg gcatccgcct
     1261 gcgcgcaaac gcaccaggca gacagcagta gtgagagcag cccccgcttc atgacctgcg
     1321 gatagtagcg cgtgccaagc ccgcgaaact gcggaacaat gcggcaactt cgaaccagga
     1381 ggccccatga aaactcttgc gctgatcgtc tcgctggccg ccggcacttc tttcgcggcg
     1441 gaaaacgcgc ctcccgccaa ggtcacgccg gtcatgacgc gcgatctcga aggcattccc
     1501 ggcaaggaga tgctggtgct ggtcgtcgaa tatccgcccg gcggtgtgtc ggcgccgcat
     1561 cggcacgacg catccgtgtt cgtctacgtg ctcgaaggcg cggtcaccat gcaggtggcc
     1621 ggcagcgcgc ccgtcacact caaggccggc ggatcgttct acgagactcc tggtgatgtg
     1681 cacgtgacgt ccgccaatgc gagcagcagc gagccggcga aatttctcgt gtacatgctg
     1741 aagaacaaag gcgcaccgcc gtcgacgccg gtgaccgggg tacagccggg cggttgaaca
     1801 aaaggtgctg cctgccctcc tcgaccaata cggctacctg ctgatcttcg tcggcacgtt
     1861 cgtcgagggt gaaaccttgc tgatgctcgg gggctacttc gcgcatcgcg gctacctcga
     1921 tctcgccgga gtcatggcga cggccttcgc ggcggcggtg tgcggcgacc agctcttctt
     1981 ccatatcggg cgccgccatg ccacgcggtt gttcgagcgc tttccgcgac tgcacgacaa
     2041 ggtgcgcgtc gccgtgcatc gtgccgagcg ccaccagact ctcgtcgtgt taggcatgcg
     2101 tttcctgtgg ggtttgcgta tcgcgatgcc gctcgcgatg ggcatgacgc gcatggacgg
     2161 gcggaagttc ttctggctca acctgatctc ggcggcggtg tggtcggtgc tgttcgcgat
     2221 gctgggcttt gccgggagcc agcttttcac gcatctcatc gacgatctgc atcgatacga
     2281 gctggggatt gccggtggcc tggtggggat cggcgtcgtg gtgttgggca tacgctggct
     2341 cggggcccga cgcccggagc agatgcacga cgagtgaagg agtcgtccta caccccctag
     2401 caacctgccc gcactatcct agcgcgacga tgagcgccaa agaccccgtg ttccctgccg
     2461 ccgacgacgc gcaaagccgc ctggcggccg tgatcgagtt ttcggatgac gccatcatca
     2521 ccaagacgct caatggcgtc atcaccacct ggaatcccgg cgcggcacgg atcttcggat
     2581 tcaccgccga agaagcgatc ggtcagcccg tcaccatgct gatccccgag aatcacctcg
     2641 acgaggagcc tgacatcctg aatcggctgc ggcgcggtga acgaatcgag cactacgaaa
     2701 cggtccgccg ccgcaaggac ggcacgctga tccaggtgtc gctcaccgtc tccccgatca
     2761 aggacgcgac aggccgcgtc atcggcgcct cgaaaatcgc ccgcgacatc acgcgccaga
     2821 agcgcgccga agcgctgatc cgcgagcagg ccgatgtgct cgccttgctg gacacgacgg
     2881 gcaaggcgat cggttccaag ctcgacctgc agaatgtatt gcagacggtc accgacaccg
     2941 cgacgcatct gaccggcgcg aagttcggcg cattcttcta caacgtgatc aacgaacacg
     3001 gtgaagcgtt cctgctgtac accctgtccg gcgcgtcgcg cgaatccttc gagaagttcg
     3061 gcctgccgcg caacaccgcc gtgttccacc ccacgttcac cggcgccggc gtcgtgcgct
     3121 cgccggacat cacccaggat ccgcgctacg gcaagatgag cccgcactac ggcatgccga
     3181 aaggacattt gccggtgcgc agttacctcg cggttccggt catctcgcgc aacggcgaag
     3241 tcatgggcgg attgtttttc ggacatcccg aagtggacca attctccgaa cgcgccgagc
     3301 aactcgtcgt cggagtcgcg agccaggcgg ccgtggccat ggacaacgca agactctacg
     3361 aagccgcaca acgcgagatc gccagccgcg agcgcgccga agccgcgctg cgcgagaccg
     3421 atcaacgcaa ggacgagttc ctggcgacgc tggcgcacga gctgcgcaat ccgctcgcgc
     3481 ccattcgcca ggcgacgctg atctcgatgt cggcgagcgc cacggaagat cagaagcggt
     3541 ggagtcacga ggtgatcacg cggcaggtgc atcacatgtc gctgctgctc gacgacctgc
     3601 tggatatttc gcgcatcacg cgcggaacac tcgagctgcg tactgagatg accgacctcg
     3661 gagcggtggt cgacgccgcg gtggagacct cgcgtccggc catcgaagcc aagatgcacc
     3721 agttcgtcat cgaccttccg cgcgagcccg tgaagtttgc ggcggacccg ttgcgcctcg
     3781 cgcaggtcct gtcgaacctt ttgaccaacg cggccaagta caccgatccg cgcggcacca
     3841 ttcgactgcg cgcctccgcc gatgcggaca acgtcacgat taccgtcgcc gataccggca
     3901 tcggtctttc gagtgacgcg ttgtcggccg tgttcacgat gttctcgcag gtgcagtcca
     3961 cgcgagatcg ctcggacggc ggattgggca tcgggctcgc gctctccaag ggcctggttc
     4021 agcttcatgg cggcaccatc ggcgcgagca gccccgggcc cggccgtggc agcgagttcg
     4081 tcgtgaaaat tccacgcgtc aggatcgcaa gcgccacgac atcggactcg gcgcgggccg
     4141 gtgaggcgcc gcgtcgtgtg cgccgggtgt tgatcgccga tgacaatcgc gatgccgccg
     4201 agagcctggc catgctgctc gaaatgcagg gccacgcgat caccgtggtg cacgacggcc
     4261 ggcaggcgct ggcggcgatc gaggaatctc cgcccgaagt ggcgctgctc gacatcggca
     4321 tgccgcacat cgacggctac gaagtcgcgc ggcaagtgcg cagcgacacg cgtcgccaga
     4381 acgttctgct cattgccgtt accggctggg gccaggaaca ggacaaggcg cgcgccatgg
     4441 ccgccggttt cgacctgcat ttcaccaagc ctatcgaacc gcagcggctg ttcgacctgc
     4501 tgggcgcaga gttgcccacc cgataaggac tacaatgccg catggcattg agcgtcttcg
     4561 accttttcaa gatcggcatc gggccttcga gctcgcacac cgtgggaccc atgcgcgcgg
     4621 cgcggcagtt cgcgctgcgg ctgcgagatc agcgcgtcgc gagcatcagc gtcatgctgt
     4681 acggctcgct cggcgccacc ggcaagggcc acggcaccga caaggccatc ctgttaggcc
     4741 tgtcgggcca tgaacccgac actgtcgacg tcgatgccat cgccggaatc atcgaacgca
     4801 tccggacgac gcacgaactg caattgctcg gcacgcatgc gctgccgttc gacgagcgcc
     4861 gcgatctcac gttcaaccac aaggacacgc tgccgctgca ttcgaacggc atgttgttca
     4921 aggcgttcgc ggaagacggt tccgagctca ctaccggcac gtattactcg gtgggcggcg
     4981 gcttcgtcgt ttccgaaagc gaatcgccgg gcggcggcac tgaaaagcgc atcgtgccgg
     5041 atctcaccgt gctgcccttc ccgttccgca acggcgacga gttattggcg ctgtgtgaaa
     5101 gagagaagtc gagtattgcg agcatcatgc gtcgcaatga acgcgtctgg cgcaccgacg
     5161 cggaaatcga ccaggggctg ctgcacatct ggcatgtgat gcaggcctgc gtgaagcgtg
     5221 gctgcgaaac gccgggcgtg ctgccgggtg gctacaaggt gaagcgacgt gcgccggatc
     5281 tatacgcaca gctcaccaag cctgcgagcg ccgcgccgca cgacccgttg atagcgctcg
     5341 actgggtgaa tctgtgggcg ctggccgtga atgaagagaa cgccgccggt gggcgcgtgg
     5401 tcaccgcgcc gacgaatggc gccgccggca tcgtgccgtc gctcatgcat tactacgcgc
     5461 gtttcgtgca cggcgcgaat gatgccggca tcgtcgattt cctgctgacc gccgcggcga
     5521 tcggcttcct atataaagag aatgcttcga tctcgggcgc cgaagtcggg tgccagggtg
     5581 aagtgggcgt ggcgtgttcg atgtcggccg cggcgctgac ggccgtgctc ggcggaaccg
     5641 tggcgcaggt ggagaacgcc gcggagatcg cgatggagca tcacctgggt ctcacctgcg
     5701 atccggtggg tgggctcgtg cagattccgt gcattgaacg aaatgccatc ggcgcggcca
     5761 aagccatcaa cgtggcgcgc atggcgctgc gcggcgatgg cacgcattac gtgtcactcg
     5821 acaaggtcat caagaccatg cgcgagaccg gcgccgacat gaagacgaag tacaaagaga
     5881 cgtcgcgc
//
LOCUS       DATDEU010000655         4122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_128330, whole genome shotgun sequence.
ACCESSION   DATDEU010000655 DATDEU010000000
VERSION     DATDEU010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128330"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VIV63_15615"
     CDS             complement(<1..496)
                     /locus_tag="VIV63_15615"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126081.1"
                     /translation="MKKETLIHLAAAIAVGIGLRFVVNLEPVWWLAWFVPGLLLALAL
                     RTENWTSRGLVALAAAIGVTANVPFFLKVMPPVPVVIVMTLQTLLWVFVVGTARRVIK
                     AFDSGWTVLAFPVICVGADTLLAHFTPDGNWGSVAYTQSDVLPIAQLASVFGVGGVLF
                     VVMLM"
     gene            complement(506..1978)
                     /locus_tag="VIV63_15620"
     CDS             complement(506..1978)
                     /locus_tag="VIV63_15620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6126082.1"
                     /translation="MNNTKPQVTFWQIWNMCFGFLGIQFGFALQNSNVSRIFQTLGAS
                     IEELPILWVAAPLTGLLVQPIVGYYSDRTWNWLGRRRPYFLAGALLASIALICMPRSP
                     TLWVAAGLLWVLDASINIAMEPFRAFVGDQLPERQRPSGYLMQSFFIGVGSVIASLLP
                     WLFEKMGVSNVGTGSGAASIPDTVRFSFDIGAAVLAGAILWTVITTREYPPAELHGFA
                     DSTPPSEGVVPGGAAHAASRGALWLGVGIAGALLVWHFERRAELYILCGLFGGWGLAL
                     LLNRARSSDGMFATLMRDIDGMPGRMRQLIPVQFFSWLALFAMWLYTNAAVTQVHFHA
                     TDTVGAAYNEGANWAGVLFAAYNGFAALAAILIPVMVRRFGLAVSHCINLVLGGVGLA
                     SFLLIRDPDWLLASMVGVGFAWASIVSLPYALLAGSVPSRKMGVYMGVFNFFIVIPQL
                     VAASVLSDVLKPLLGGQSINVLLVGGVSFAIAGVLALRVR"
     gene            complement(1975..4074)
                     /locus_tag="VIV63_15625"
     CDS             complement(1975..4074)
                     /locus_tag="VIV63_15625"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEY6126083.1"
                     /translation="MRLRLWWVLAAFAFQAAHAQGVTGIAANAVRSPDGRMVVALRMD
                     GDGRPEYSIQRNGRAVIDWSHLGFILADAPKLERNFALATAEETAIDETWEQPWGERR
                     FVRNRCIEKRFTLREKTGGQRSLTVVFRVYDDGVGFRYEFPDQPSLTQVGIVDELTEF
                     AVAELATAWWIPGGEWNRYEYLYQKTPLTEVGQAHTPMTMKTASGLHIAIHEAALVDY
                     AGMWLRRVSGQRLKALLSPSSSGPKVSRKAPFATPWRTLQIADSAAGLYMSDLVLNLN
                     EPNKLGDVSWVKPFKYVGIWWSMHLGTHTWASGPKHGATTKETMRYIDFAATHGFRGV
                     LVEGWNKGWDGDWFAKGDEFSFTEAYPDFDIVALAAYAKKKGVHLVGHHETGGNIAVY
                     EKQLGPALDLYGKLGIDAVKTGYVADAGGVQALGDDGRIHFEWHDGQVQSRHHLRVVE
                     EAARRHIAVNAHEPIKDTGLRRTYPNWVSREGARGMEYNAWGDPKNPPEHEVNLVFTR
                     MLSGPMDYTPGILSLTGAGGKPIQSTIARQLALYVVLYSPIQMAADLPENYAKHMKPF
                     QFIKDVPVDWSDTRVLNGEVGDYATIARKDRHSDDWYLGAITDEEPRTLSASLGFLDE
                     GKKYYATVYRDGKNASVEIETRPVSRFDTLQLVLASGGGQAIRISTNLGSRGPRSSVK
                     PAPKPAAKKPQPEFDVE"
BASE COUNT          800 a         1397 c         1254 g          671 t
ORIGIN      
        1 tcatcagcat gaccacgaac aacacgccgc ccacaccgaa taccgacgcg agttgcgcga
       61 tgggcagtac atctgactgc gtgtacgcga cgctgcccca gttgccgtcg ggcgtgaagt
      121 gcgcgagcag cgtgtccgcg ccgacgcaga tgacggggaa tgcgagcacg gtccagccgg
      181 agtcgaacgc cttgatgacg cgcctcgcgg tgcccacgac gaagacccac agcaacgtct
      241 gcagcgtcat gacgatgacg acgggtaccg ggggcatcac cttgaggaag aacggcacgt
      301 tggccgtgac gccgatggcg gccgccagcg ccaccagccc tcgcgaggtc cagttctccg
      361 tgcgcagcgc caaggccagc agcagacctg gcacgaacca cgcgagccac cacaccggtt
      421 ccagattgac gacgaagcgc aggccaatgc cgaccgcgat ggccgcggcc aggtgaatca
      481 acgtctcttt cttcatgccg gcagtctaac gaacccgaag cgcgagcacc cccgcaatgg
      541 cgaaactcac gcctccgacc agcaatacgt tgatcgactg gccacccagc agaggcttca
      601 acacatcgct caacacactc gcggccacga gctgcgggat cacgatgaag aaattgaaca
      661 cgcccatgta cacgcccatc ttgcgcgacg gaacgctgcc ggccagcagt gcgtacggta
      721 gcgagacgat cgacgcccag gcgaatccga ctccgaccat cgacgcgagc agccagtccg
      781 gatcgcggat cagcaggaag gacgcgaggc cgacgccacc caacaccagg ttgatgcaat
      841 ggctcacggc gagaccgaac cgcctcacca tgacgggaat caggatcgcg gccagcgctg
      901 cgaatccgtt gtacgcggcg aataacacgc ccgcccagtt cgcgccttcg ttgtaagccg
      961 cgcccacggt gtcggtggcg tggaaatgaa cctgcgtcac ggccgcgttc gtgtacagcc
     1021 acatcgcgaa caacgccagc cacgagaaga actgcacggg gatgagctgc cgcatgcgcc
     1081 ccggcattcc atcgatgtcg cgcatcaggg tcgcgaacat gccgtcgctc gaacgcgcgc
     1141 ggttgagcag caacgcgaga ccccagcctc cgaacaaccc gcagaggatg tacagctctg
     1201 cgcgccgctc aaaatgccag acgagcaatg cgccggcgat gccgaccccg agccacaacg
     1261 cgccacgaga cgccgcgtgt gcggcgccgc cgggtacgac gccttcggag ggcggcgtcg
     1321 aatcggcaaa gccatgtagt tcggcgggcg ggtattcgcg cgtcgtgatg acggtccaca
     1381 aaatggcgcc ggccagcacc gcagcgccga tgtcgaatga aaaacgaacc gtgtccggga
     1441 tcgaggccgc gccgctcccc gtgcccacgt tgctgacgcc catcttctcg aacagccacg
     1501 gcagcaggct ggcgatgacc gagccgacgc cgatgaagaa gctctgcatc aggtacccgg
     1561 aaggacgttg ccgctccggc aactgatcac cgacgaacgc gcggaacggc tccatcgcga
     1621 tgttgatgct ggcatcgagc acccacaaca atccggcggc gacccacaac gtgggcgacc
     1681 gcggcatgca gatcagcgcg atcgacgcga gcagcgcacc ggcgaggaaa tacggccgcc
     1741 gccggccgag ccagttccag gtccgatcgg agtagtaacc gacgatcggc tgcaccagca
     1801 acccggtgag gggcgcggcg acccacagga tagggagctc ctcgatggac gcgcccaggg
     1861 tctggaagat gcggctgacg ttcgaattct gcagcgcaaa gccgaactgg attcccagga
     1921 acccgaagca catgttccag atctgccaga acgtgacctg aggtttggtg ttgttcattc
     1981 gacgtcgaac tcgggttgcg gcttcttggc cgcgggcttt ggcgcgggct tgaccgatga
     2041 tcgcggcccg cggcttccga ggttcgtcga gatgcggatc gcctggccgc caccggacgc
     2101 gagcaccagc tgcaacgtgt cgaagcgcga cacgggccgg gtttcgattt cgacgctggc
     2161 gttcttgccg tcccgataga ccgtggcgta gtacttcttt ccctcatcga gaaaacccag
     2221 cgacgcgctc aacgtgcgcg gctcttcatc cgtgatggcg cccaggtacc agtcgtcgct
     2281 gtggcgatcc ttgcgcgcaa tcgtggcgta gtcacccacc tcgccgttga gcacgcgagt
     2341 gtcgctccag tccaccggca cgtccttgat gaactggaac ggtttcatgt gtttcgcgta
     2401 gttctccgga aggtccgccg ccatctggat agggctgtag agaacgacgt acagcgcgag
     2461 ctgccgcgcg atcgtcgact ggataggttt accgcccgcg cccgtgagac tcaggatgcc
     2521 cggcgtgtag tccatcgggc cggagagcat gcgcgtgaac accaggttca cctcgtgctc
     2581 cggcggattc ttcggatcgc cccaggcgtt gtattccatg ccacgcgcgc cctcgcgcga
     2641 cacccagttc ggatacgtgc gccgaagtcc cgtgtccttg atcggctcat gcgcgttgac
     2701 cgcaatgtgg cggcgcgccg cttcctccac gacacgcagg tgatgccgcg actgcacctg
     2761 gccatcgtgc cattcgaaat ggatgcgccc gtcgtcaccc agcgcctgta ctccgccggc
     2821 gtcggccacg tagccggttt taaccgcatc gatgccaagt ttcccgtaga ggtcgagcgc
     2881 gggacccaac tgcttctcgt agaccgcgat gttgcccccg gtttcatggt gacccacgag
     2941 gtgcacgccc ttcttcttcg cgtacgccgc cagcgccacg atgtcgaagt ccggataggc
     3001 ctcggtgaag ctgaactcat cacccttggc gaaccagtcg ccgtcccacc ccttgttcca
     3061 gccttcgacg agcacgccac gaaaaccgtg cgttgccgcg aaatcgatgt aacgcatcgt
     3121 ttccttcgtg gtcgcgccgt gtttcggacc cgacgcccac gtgtgcgtgc ccaggtgcat
     3181 gctccaccag atgccgacgt acttgaacgg cttgacccag gagacatcgc ctagcttgtt
     3241 aggctcgttg agattgagga cgaggtccga catgtagaga ccggccgcgg agtcggcgat
     3301 ctgcagcgtg cgccacggag tcgcgaacgg cgccttgcgg ctcaccttcg gtccgctcga
     3361 cgatggtgaa agcaacgcct tcaaacgctg cccggaaacc cgccgcagcc acatgccggc
     3421 atagtcgacg agcgccgcct cgtgaatcgc gatgtgcagg ccgctcgccg ttttcatcgt
     3481 catcggcgtg tgagcctgcc cgacctccgt cagcggcgtc ttctggtaga ggtattcgta
     3541 gcggttccac tcgccgcccg gaatccacca cgcggtggcc agctccgcga ccgcgaactc
     3601 ggtgagctcg tcgacgatgc cgacctgcgt caacgacggc tgatcgggaa attcataccg
     3661 gaagcccacg ccatcgtcgt acacgcgaaa caccacggtg aggctgcgtt gcccaccggt
     3721 cttttcgcga agcgtgaatc gcttttcgat acagcgattc cgaacgaagc gccgctcgcc
     3781 ccagggctgc tcccaggttt cgtcgatcgc cgtctcttcg gcagttgcca gcgcgaagtt
     3841 gcgttcgagc ttgggggcgt cggcgaggat gaatcccaag tgcgaccagt cgatgaccgc
     3901 gcgtccgttg cgttgaatgg aatattcagg ccggccgtcg ccgtccatgc gcagcgcaac
     3961 gaccatcctt ccatccggcg accgtaccgc gttcgccgca atgccggtca cgccctgcgc
     4021 gtgagccgcc tgaaacgcaa acgccgccag cacccaccac agacgaagcc tcattgcgcg
     4081 ccgcccgcac aaacgacata gccgagtgcc ggcacttcca cc
//
LOCUS       DATDEU010000656         4213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1295710, whole genome shotgun sequence.
ACCESSION   DATDEU010000656 DATDEU010000000
VERSION     DATDEU010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295710"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4213
                     /locus_tag="VIV63_15630"
     CDS             <1..>4213
                     /locus_tag="VIV63_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY6126084.1"
                     /translation="DRVLAGGRDLALVLLPLLNDMRAVRGSPLLSEGTLLLLNLKSDQ
                     QAAPVAPNAGEPALTVAQWARRLRTRFQLGLVGWIRGERPEQNLEILETVAHQLEQVA
                     TRQSVFQLWWVVGAIVEALREGGLETGVSVKRLLGLADREILQLYTQGEGRYSQNPPV
                     ELLNNLLYYAARATSNGPKVTAVRASFRLNELLNVDDSVEQERENLSAPSVKLMRTVS
                     AAIREDLGKVKDVLDIFVRRGGQPVELEPQVGMLRKIADTLGVLGLGELRQLVIEETN
                     RLEAMAAGKTASDHAALVHIAATLINVEDRLDRDLIGLIVPKTAQPQTEEGSVDVDFQ
                     QVQSAVLRECSVNLVRVKEAIAANVAGTLDVGALDAWPGLIAGIKAGLLMLGKSRAAD
                     IVEGIARNLRELLQPGGTAVPPNYLDRLADAVVSLEYYMETLQAGRADPWYMLDNAQT
                     CLQALSQMPKRVVPTVPPLGPEAYAATVLLADAGATARAHALQAGVAPSLQGSAPTLH
                     HSAQPPIGAKPINIDPELLTLYIEEAREEVARIAKLFPAWEQNPLETDALAGVRRAFH
                     TLKGSGRMVGATDISEFAWSIENLLNKIIENTLQRSPAILATIRDASAIAGELVNALE
                     AGQGAPAKVQEIIDRAHALAANRASPGAQTATMEILERTLETRRDDVAAATSRVPTLQ
                     PPPPAPVTAFAAQPEPVVEAEGSIEVVEDWSTATQDGGENIVLSAPEEEPSGDMQLRD
                     IYSRETEVNIAAVMRFIDAERSRSAPHVISEEAYRACHTLSGSSRMAEARHGIRITAP
                     LEHWVRKSFDSGVGLEDPELALLADCMTAMQAVASHLDESTGFFQSHSALLTRIDQAT
                     ENLEQRIIAAARVAEATAAQPQMAAPTVSVAPAVAAPPASFPQVAPATAAPAHAVEDV
                     STDFDQDIASIFTDEAVELIEQAQSALAQWNENRTSVDGLTALKRPLHTLKGGARMAG
                     LMPMGDLSHELETLFMQIDSGVVAPDDRAFSLAQTSLDELARMRESVSSGKGVPTANG
                     LIARIHALVTGAPIEAAPAPTPTPAPMPAPAPVQAAAPPPPPPPSVVAAAPAPVTPAP
                     KPAPAPTISAVPSPPKPAYVPPPPRVEPMPEPPVLAAVWAPEPPSIVTRAAARDPISA
                     GFNREAANKDDAPSDGPLLPPGAVPPGREPTAPSDRPELARVDAELLDQLLNNSGEAS
                     IARARLEQQIGSIDFNVGELSRTVTRLKEQLRKLELETEAQILHRFEEEKGQKSDFDP
                     LELDRYSSIQQFSRGLAETANDVGSIQQLLENLTKDTQNLLTQQARTITELQNGLMRT
                     RMVPFQRHVQRLARIVRQAATDTHKKAELQIEGASGELDRQVLERMMPPFEHMLRNAV
                     AHGIEKPEDRKKAGKSESGTITIALH"
BASE COUNT          766 a         1526 c         1311 g          610 t
ORIGIN      
        1 cgatcgagtg cttgcgggtg gacgcgacct ggccctggta ctgctgccac ttctcaatga
       61 catgcgcgcc gtgcgtggca gcccgctgct gtcggaaggc acgctgctgc tgctgaatct
      121 caaatccgat cagcaggcgg cgcccgtggc gccgaatgcc ggtgagcccg cactcacggt
      181 cgcgcaatgg gcgcgccgtc tgcgcacgcg attccagctc ggtctcgtcg gctggatccg
      241 cggcgagcgg cccgaacaga acctcgaaat cctcgaaacc gtggcgcacc agctcgagca
      301 ggtggcgacg cggcagtccg tgttccagct ctggtgggtg gtcggcgcca ttgtcgaggc
      361 actgcgcgaa ggcggcctcg aaaccggcgt gtcggtcaaa cgccttttgg gtctcgccga
      421 tcgcgagatc ctgcagctct acacgcaggg cgaagggcgc tactcgcaga acccgcccgt
      481 cgagctgctg aacaatctcc tctattacgc cgcgcgcgcc accagcaatg gacccaaggt
      541 cacggcggtg cgcgcctcgt tccggctcaa cgaactgctg aacgtcgacg actcggtcga
      601 gcaggagcgc gagaacctct ccgcgccgtc cgtgaaactc atgcgcacgg tctccgcggc
      661 catccgcgaa gacctcggca aggtcaaaga cgtcctcgac attttcgtcc gccgtggcgg
      721 acagcccgtc gagctcgagc cgcaagtggg catgctgcgc aagatcgccg acacgctcgg
      781 cgtgctcggc ctgggcgagc tgcgtcagct cgtcatcgaa gaaacgaatc gtctcgaagc
      841 catggccgcc ggcaagaccg cgtcggacca tgcggcgctg gtgcacatcg ccgccacgct
      901 catcaacgtc gaagaccgcc tcgatcgcga tctcatcgga ctcatcgttc cgaagaccgc
      961 gcagccccag acagaggaag gctctgtcga cgtcgacttc cagcaggttc agtccgcggt
     1021 gctgcgcgag tgcagcgtca acctggtgcg ggtcaaggaa gccatcgcgg ccaacgtcgc
     1081 cggcacgctg gacgtcggcg cgctcgatgc ctggccgggc ctcatcgccg gaatcaaggc
     1141 gggactgctc atgctgggca agtctcgcgc ggccgacatc gtcgaaggca ttgcgcgcaa
     1201 tctcagggag ctgctgcagc ccggtggcac ggccgtaccg cctaactacc tcgaccgcct
     1261 cgcggacgcg gtggtcagcc tcgagtacta catggagacg ctgcaggccg gccgcgcgga
     1321 tccctggtac atgctcgaca acgcgcagac ctgcctgcag gcgctgtcgc agatgcccaa
     1381 gcgtgtggtg cccacggtgc cgccgctcgg ccccgaagcc tatgccgcga cggtgttgct
     1441 ggccgacgcg ggcgccacgg cccgcgccca tgcgttgcag gccggagtgg cgccctcgct
     1501 ccagggttcc gcgcccacgc tgcatcattc ggcgcagccg cccatcggcg ccaagccgat
     1561 caacatcgat cccgagctgc tcactctata tatagaggaa gcccgggaag aggtcgcccg
     1621 tatcgccaag ctgttcccgg cctgggaaca gaacccgctg gaaaccgatg cgctcgccgg
     1681 cgtgcgccgc gctttccaca cgctcaaggg ctccggccgc atggtcggcg ccaccgacat
     1741 cagcgaattc gcctggtcca tcgagaacct gctcaacaag atcatcgaga acacgctgca
     1801 gcggtctccg gccattctcg ccaccattcg cgacgccagc gccatcgccg gcgagctggt
     1861 gaatgccctc gaagcaggac agggcgctcc ggcgaaggtg caggaaatca tcgatcgcgc
     1921 gcacgcgctg gccgccaacc gcgcgagccc cggcgcgcag accgcgacga tggaaattct
     1981 cgagcgcacg ctcgaaacgc gtcgcgacga cgtcgcggcc gctaccagtc gtgtgcccac
     2041 gctgcagccg ccgccgccgg cgcccgtgac cgccttcgct gcgcagcccg agccggtcgt
     2101 cgaggccgaa ggttcgatcg aagtcgtcga agactggtcc accgccacgc aggacggcgg
     2161 cgaaaacatc gtgttgtccg cgccggagga agaacccagc ggcgacatgc agcttcgcga
     2221 catctacagc cgcgagaccg aggtgaacat cgccgcggtc atgcgcttca tcgacgccga
     2281 gcgttcgcgc agcgcgccgc atgtcatcag tgaagaggct taccgcgcct gtcacacgct
     2341 gtccggcagt tcacgcatgg ccgaagctcg tcatggcatc cgcatcacgg cgccgctcga
     2401 gcactgggtg cgcaaatcgt tcgacagcgg cgtcggcctc gaagatccgg agcttgcgct
     2461 gctcgcggac tgcatgactg ccatgcaggc ggtcgcctcg catctcgacg aatccacggg
     2521 cttcttccag tcgcacagcg cgctgctcac acgcatcgac caggccaccg aaaatctcga
     2581 acaacgcatc atcgcggccg cgcgagtagc cgaagccacc gctgcgcagc cacagatggc
     2641 ggcacccacg gtttccgtcg cgccggcggt cgcggcccct cccgcttcct ttccgcaagt
     2701 agcgccggcc acagctgctc ccgctcatgc cgtcgaggac gtgagcacgg atttcgatca
     2761 ggacatcgcc agcatcttca ccgatgaagc cgtcgagctc atcgagcagg cgcaaagcgc
     2821 gctggcgcag tggaacgaaa accgcaccag tgtcgacgga ctcaccgcgc tgaagcggcc
     2881 gctgcacacg ctgaagggcg gtgcgcgcat ggccggcctc atgcccatgg gcgacctgag
     2941 ccatgaactg gaaacgctgt tcatgcagat cgacagcgga gtcgtcgcgc ccgacgatcg
     3001 cgctttcagc ctcgcgcaga cttcactcga cgaactcgcg cgcatgcgcg aatcggtgag
     3061 ctcgggcaaa ggcgttccga ccgccaacgg cctcatcgcg cgcatccatg cactcgtgac
     3121 cggcgcgccc atcgaggcag cgccggctcc cacgcccaca ccggccccga tgcccgcgcc
     3181 agcgcctgtg caggctgcag cgccgcctcc gccgccaccg ccgtcggtgg tcgcggcagc
     3241 acccgcgccc gtgacgccgg ctcccaagcc cgctccggcg cccacgattt ccgcggtgcc
     3301 cagcccgccg aaacccgctt atgtgccgcc gccgcctcgc gtcgagccca tgcccgagcc
     3361 gcccgtgctc gcagccgtat gggcacccga gccgccgtcc atcgtcactc gcgctgcagc
     3421 cagggacccg atctccgccg gcttcaatcg cgaggccgcc aacaaggacg acgcgcccag
     3481 cgatggcccc ctgctgcctc cgggcgcagt gccaccgggc cgtgaaccga ccgcgccttc
     3541 cgatcgaccc gaactcgcgc gcgtcgacgc ggaactgctc gatcagctcc tcaacaattc
     3601 gggcgaagcg agcatcgcgc gcgcgcgcct cgagcagcag atcgggtcga tcgatttcaa
     3661 cgtgggcgaa ctgtcacgca cggtgacgcg tctgaaggaa cagctccgca agctcgagct
     3721 cgagaccgaa gcgcagatcc tgcatcgctt cgaagaagag aaaggccaga agagcgactt
     3781 cgatccgctc gagctcgacc gttattcctc gatccagcag ttctcgcgcg gcctcgccga
     3841 aacggcgaac gacgtcggca gcattcagca gctgctcgag aacctcacca aggacacgca
     3901 gaacctgctc acccagcagg cgcgcaccat caccgaattg cagaacggcc tcatgcgcac
     3961 gcgcatggtt ccgttccagc gccacgtgca gcgcctcgcg cgtatcgtgc gccaggccgc
     4021 gacggacacg cacaagaagg ccgagctgca gatcgaaggc gcttcgggcg aactcgaccg
     4081 ccaggtgctc gagcgcatga tgccgccgtt cgagcacatg ctgcgaaacg cggtcgcgca
     4141 cggcatcgag aagcccgaag accgcaagaa ggcgggcaag agcgaaagcg gcacgatcac
     4201 cattgccctt cat
//
LOCUS       DATDEU010000657         6718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_568434, whole genome shotgun sequence.
ACCESSION   DATDEU010000657 DATDEU010000000
VERSION     DATDEU010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568434"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VIV63_15635"
     CDS             complement(<1..789)
                     /locus_tag="VIV63_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126085.1"
                     /translation="MKILLARVLIGLTVFSGLAWAQAPSVRARSTTTVVSPTRAAPAL
                     PAAAKPSESVKEQVPIPRVSDPRDTYTPRPPLRLAIAPSIAAVYQPLNQHVEVFGIDP
                     KGALKDVWKHHNGFWEAAFYLSPPGFAPPGAPLAAIWYPLNEQLEVFTIGMNGALTVT
                     YKVRNGPWFAPAYITPPGFAQPGAHVAAVFQPLNNQLEVFAIDATGAVKIAWKAQNGR
                     WQGPVALTPPGIAPAGAPITAVWQPLNEQLEVFWVDTAGSLRGIW"
     gene            complement(885..1799)
                     /locus_tag="VIV63_15640"
     CDS             complement(885..1799)
                     /locus_tag="VIV63_15640"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6126086.1"
                     /translation="MEALLQFIEIYGLWVVFVCVLLDQGGLPFPAYPPMIVTAAMAVD
                     GHESLAAILWVAIFAAVLADVLWYACGRRYGPAMLRLMCKVSISPDSCVGATRRVYAR
                     WGPESLIFAKYVPGLAAVATTLAGESRLSVTRFIILDGIGAALWAAGAVALGAIFHDA
                     VEAVLLTLEQFGSFALLLLALTVALYIAFKWRKRHRFLMQIQMARLTPEELETRIREK
                     SDLKILDVRPAGRRAQQGWIPGSILIADFEGGQPDKKTEVVVYCDCPNDASAAVAALR
                     LKDMGFTNVRPLAGGIDAWSRGGRVLDR"
     gene            1937..2125
                     /locus_tag="VIV63_15645"
     CDS             1937..2125
                     /locus_tag="VIV63_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126087.1"
                     /translation="MKTSESTPAPIPKHDYDQALKSAVSWLGDRYLLAEPLRKRRDEP
                     KDYFVQARRWHSAVNTAR"
     gene            2220..3464
                     /locus_tag="VIV63_15650"
     CDS             2220..3464
                     /locus_tag="VIV63_15650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6126088.1"
                     /translation="MNTPTAPAIASFSGYQKVVVGMLAFLQFAVILDFMLMAPLGALI
                     MPALSVTPAQFGTVVSAYAFAAGISGLLTAGFADRFDRKKLLLVFYAGFIVGTAWCGV
                     AQSFESLLLARIVTGLFGGVIGSVVLAIATDLFPPQMRGRVMGFIQTAFAASQILGIP
                     IGIYLSNQWNWHVPFLALVVLGVIGGLLIAFYMKPVADHLKVPQEHSAFNHLRHTVTE
                     PRYLMSFVSVLFLATGGFMLMPFSSAFTVNNLGISLHDLPIIYLVTGIATIFVGPIVG
                     KAADAFGPLRVFFVGTALTIIMVLIYTHLGVSTLVIVTAVNVVLFIGIFSRMIPFQAL
                     MSQVPVQTQRGSFNAINASISQLAGGVASLVAGHIVQQGADGHIYHYEVAGYVVVGTS
                     LLACFLMWRIQRGITAQAATST"
     gene            complement(3436..4047)
                     /locus_tag="VIV63_15655"
     CDS             complement(3436..4047)
                     /locus_tag="VIV63_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6126089.1"
                     /translation="MILPGAVGAASPARSEYQAAIRAVPDLEHGALLFAQCVSCHGAD
                     GQGLPDGSTPRIAGQHFNVLVKQLVDFRYGKRWDFRMEQRANTHLGAFQDIADVAGYL
                     GQQPRKNSGIRPGNDNLAQGANLFEANCATCHGARGEGDDVAIVPMLAGQHPAYLLRQ
                     MYDSVDGRRPSLAKVHAPRIKPLDFAQLHAIADFLTSMSPPAP"
     gene            complement(4080..5129)
                     /locus_tag="VIV63_15660"
     CDS             complement(4080..5129)
                     /locus_tag="VIV63_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY6126090.1"
                     /translation="MKALVYRGPGLKAIEDRPKPLIQSPGDAIVKISLTTICGTDLHI
                     LKGHVPTCAPGRILGHEGVGIIESVGAGVTALKPGDHVLISCVSSCGRCENCRRGMYS
                     HCATGGWILGNEIDGTQAEYVRTPHADTSLYPIPAGAAEEALVMLSDILPTGFECGVL
                     NGKVAPGSTVAIVGSGPIGLATLLTAQFYSPAQIIVIDLDDKRLEVARRFGATSTINS
                     ADGNAAATVKTLTGGRGVDTAIEAVGIPQTFSLCQELVAAGGVIANIGVHGEKVDLHL
                     ERLWSHNISITTRLVDTVSTPMLLKTVQSGKIDPARLITHRFKLAQILEAYDVFARAS
                     ETGALKVILSTGGPA"
     gene            complement(5251..6459)
                     /locus_tag="VIV63_15665"
     CDS             complement(5251..6459)
                     /locus_tag="VIV63_15665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6126091.1"
                     /translation="MKGTRYNRRRLLQSLAAAGGAALTGCATPTLIGGATPERLAPND
                     PARSLAPLRARTDRIFDLMVCLRPFRAAGPRLDTERIRDTLVVHNYGHGGSGWSLSWG
                     SSTVAVNKALTQASDRIAVVGCGALGLTSALLAQQAGKSVTIYARDVIQQTFSARASG
                     SFTPDSRIALTDAVSPEFPALWEEMARTSLKTFRGHLGLAGDPVRWIDRYAVSDSAAP
                     APRVPVEGELHFAAYGNRLQDLFPPSENLRPADTPFRAASVRRRGVLMFNLASYGQTL
                     MTQFRLAGGRIEHREFHEPQDMAALPEKVVINCTGYGARALWRDESIVPVRGQHAYLI
                     PQREFDYALAYDGVNVVPRSDGIVVQAVSGGDMRGYNDDSLAPDRRESEEAVQKLAAL
                     YGKFGRPGHR"
BASE COUNT         1210 a         2180 c         2118 g         1210 t
ORIGIN      
        1 ccagatgccg cgcaacgaac cggccgtgtc gacccagaac acttcgagct gttcgttcag
       61 cggttgccat accgcggtga tcggcgcacc ggctggcgcg atacccggcg gcgtcagcgc
      121 caccggccct tgccagcggc cgttctgcgc cttccatgca atcttcaccg cgcccgtggc
      181 gtcgatggcg aacacttcga gctgattgtt cagcggttga aacaccgccg cgacatgcgc
      241 tccgggctgc gcgaatcccg gaggcgtgat gtaggcaggc gcaaaccacg gaccgttgcg
      301 caccttgtac gtcaccgtca gggcgccgtt catcccgatg gtgaacacct cgagctgttc
      361 gttcagcgga taccagatgg ccgcgagcgg cgcgccgggc ggcgcgaacc cgggaggaga
      421 aagatagaac gccgcttccc agaatccgtt gtgatgtttc cagacgtcct tgagcgctcc
      481 cttcggatcg atcccgaaca cctccacgtg ctgattcagc ggctgataca ccgcggcgat
      541 ggatggggcg atcgccagcc tgagcggcgg ccggggcgta taggtgtccc gcggatcaga
      601 cacgcgtgga atgggcacct gctccttgac cgattcactg ggcttcgccg cggcgggaag
      661 cgccggcgcc gcgcgcgtgg gactgactac cgtggtcgtc gaccgtgcgc gcaccgaggg
      721 ggcctgggcc caggcgaggc cgctgaatac tgtcaatccg atgagtacgc gcgccaggag
      781 aattttcatg atcgtttcct tgatcgagga cgttgtctct catcagggaa tacgtggcgc
      841 gggcgaggcg ctggacacgc cacgcgaaag attggtgaca ggccctaccg gtccagcacc
      901 cttcccccac ggctccacgc gtcgattccg cccgcgagcg gtcgcacatt cgtgaagccc
      961 atgtccttca gtcgtagcgc cgcgaccgcg gcgctggcgt cgttcggaca gtcgcaatag
     1021 acgacgactt cagtcttctt gtccggctgc ccaccttcga agtcggcgat gaggatgctt
     1081 ccgggaatcc agccctgctg cgcgcggcgg ccagccggcc ggacgtcgag gatcttgaga
     1141 tcagacttct cgcgaattcg tgtctccagc tcctctggag tcaatcgcgc catctgtatc
     1201 tgcatgagga agcggtgacg cttgcgccac ttgaaggcga tgtacaacgc cacagtgagt
     1261 gccagcagca gaagcgcgaa actcccgaac tgctcgaggg tcagcagcac cgcttcgact
     1321 gcgtcgtgaa agatcgctcc cagcgcgacc gctccggcgg cccacaacgc cgcaccgatg
     1381 ccatcgagaa tgatgaaacg cgtgacactg agacgagatt cgcctgcgag cgtggtcgcg
     1441 accgccgcaa gccccgggac gtatttggca aagatgagcg actcgggacc ccagcgggca
     1501 tagaccctgc gcgtggcgcc cacacacgaa tccggcgata tcgacacctt gcacatcagc
     1561 cggagcatcg cggggccata gcgcctgccg cacgcgtacc agagcacgtc ggcgagcacg
     1621 gccgcgaaga tggcaaccca caggatggcc gcgagcgatt cgtgcccatc gaccgccatg
     1681 gcggcggtga cgatcatcgg cggatacgcc gggaacggca gaccaccctg atcgagcagt
     1741 acacaaacaa acacgaccca caaaccgtag atctcgatga actgcagcag ggcttccatg
     1801 aggcgattgt gcacgggccc gatggggacg ccagcttcct acctcctttg ggtgaccctc
     1861 gagacactcg cccgggcgtt gttggaaaca ggcccgggtc cgaaaatggc tacaccgaaa
     1921 tccggtagag actgcaatga aaacttctga atcaaccccc gcccccatcc cgaaacacga
     1981 ctacgaccag gcgctgaaga gcgcggtgtc atggctgggc gatcggtatc tgctggcgga
     2041 gccgctgcgg aagcggcgcg acgaaccgaa ggactacttc gttcaggcgc ggcgttggca
     2101 ttcggccgtg aatacggcac gttgattcgc gcactttcat gataccgccg gccacggcga
     2161 tttgaactgc cccattcggt ggaggacttc gccgtaacct gcgcgtaggg tgcgcgccca
     2221 tgaatacacc caccgcaccc gcaatcgcgt cgttttccgg ctaccagaag gtggtggtcg
     2281 gcatgctggc gttcctgcag ttcgccgtga tcctcgattt catgctgatg gccccgctgg
     2341 gcgccctcat catgccggcg ctgtcggtca cgccggccca attcgggaca gtcgtgtcgg
     2401 cgtatgcgtt cgccgccggc atctcgggcc tgctcaccgc gggctttgcc gaccgattcg
     2461 accggaagaa gttgctgctg gtcttctacg ccggattcat cgtgggcacc gcctggtgtg
     2521 gtgtcgcgca gagttttgaa tcgctgctgc tggcgcgcat cgtcacggga ttgttcggcg
     2581 gcgtcatcgg ctcggtcgtc ctcgccatcg ccaccgattt gtttccgccg cagatgcgcg
     2641 gccgcgtcat gggcttcatc cagacggcgt tcgccgccag ccagatcctc ggcatcccga
     2701 tcggcatcta tctatcgaac cagtggaact ggcatgtgcc gttcctcgcg ctggtggtgc
     2761 tcggcgtgat cggcgggctg ctcatcgcct tctacatgaa gccggtcgcg gaccatctga
     2821 aggtgccgca ggagcacagc gcgttcaatc atctgcgcca cacggtgacc gagccgcgtt
     2881 acctgatgtc gttcgtctcg gtgctgttcc tggcgaccgg tggtttcatg ctgatgccgt
     2941 tcagcagcgc gttcacggtc aacaatctcg gcatctcgct gcacgacctg cccatcatct
     3001 acctggtgac gggcatcgcg acgattttcg tgggacccat cgtgggtaag gcggccgatg
     3061 cgttcgggcc gctgcgcgtg ttcttcgtcg gcaccgcgct gaccatcatc atggtgctca
     3121 tctatacgca cctcggtgtg tcgacgctgg tcatcgtcac ggcggtcaac gtcgtgctgt
     3181 tcatcggcat cttctcgcgc atgattccgt tccaggcgct catgtcgcag gtgcccgtgc
     3241 aaacgcagcg cggttcgttc aacgcgatca acgcgtcgat ttcacagctc gcgggtggcg
     3301 tggcgtcact cgtcgcgggg cacatcgtgc aacaaggcgc ggacgggcat atctaccact
     3361 acgaggtcgc cggctatgtc gtggtcggaa cgtcgctgct cgcgtgtttc ctcatgtggc
     3421 gcatccagcg cgggatcacg gcgcaggcgg cgacatcgac gtgagaaaat ccgcgatggc
     3481 gtgcagctgg gcgaaatcga gtggtttgat gcgcggcgca tgcaccttcg cgagcgacgg
     3541 acgccgccca tctaccgagt cgtacatctg gcgcagcaga tacgcgggat gttgaccggc
     3601 cagcatgggc acgatcgcga catcgtcgcc ctcgccgcgc gcgccatggc aggttgcgca
     3661 attcgcttca aataggttgg cgccctgggc gagattgtcg ttgccaggac ggatcccgct
     3721 gttcttgcgc ggctgctggc cgagataacc ggccacatct gcaatgtcct ggaacgcgcc
     3781 aagatgcgtg ttggcgcgct gctccatgcg aaagtcccag cgcttgccgt accggaaatc
     3841 cacgagctgt ttgacgagga cgttgaagtg ctggccggcg atgcgcggag tcgaaccatc
     3901 cggcaagccc tgtccatccg cgccgtggca cgacacgcat tgggcaaaga gcagcgctcc
     3961 gtgctctaaa tcgggcacgg cccggatcgc ggcttgatat tccgaacgcg cgggcgacgc
     4021 ggccccgacg gcgccgggca ggatcatcag caggcaaact attcgcccga aagagaactt
     4081 catgccgggc ccccggttga gaggatcact ttcaaggcgc cggtctccga agcgcgggcg
     4141 aagacgtcgt acgcttcgag tatctgcgcc agcttgaatc gatgcgtgat gagccgcgcc
     4201 gggtcgattt ttcccgactg cacggttttc aggagcatcg gcgtggacac ggtgtcgaca
     4261 aggcgcgtcg tgatcgagat gttgtgcgac cagaggcgct cgagatggag atccaccttt
     4321 tccccgtgaa cgccgatgtt ggcgatcacg ccgccagccg cgaccagctc ctgacagagt
     4381 gaaaacgtct ggggaatacc gacagcctcg attgcggtat cgacaccacg acctcctgtc
     4441 agcgtcttca cggtcgccgc cgcattgccg tccgcgctgt tgatcgtcga cgttgcgccg
     4501 aatcggcgcg cgacttccag gcgcttgtca tcgaggtcaa tcacgatgat ctgcgccggg
     4561 gaatagaact gcgccgtcaa cagcgttgcg aggccgatgg gacctgaacc cacgatggcc
     4621 acggtcgagc ccggcgcaac ctttccgttc aggacgccgc attcaaagcc cgtcggcagg
     4681 atgtcgctga gcattaccag cgcctcttcg gcggccccgg cggggatcgg atacaggctc
     4741 gtgtcggcgt gcggcgtgcg gacatactcg gcctgcgtgc cgtcgatctc gttgccgaga
     4801 atccagccgc ccgtcgcgca atgcgaatac atcccgcgcc ggcagttctc acacctgccg
     4861 cacgaggaca cacaggagat cagcacgtga tcccccggtt tcaacgccgt gacgcccgcg
     4921 ccaacagact cgatgatgcc gacaccttcg tgaccgagga tgcggcccgg cgcacacgtc
     4981 gggacgtgcc ccttgagaat atgcaggtct gtcccgcaaa tcgtcgtcaa agagatcttg
     5041 acgattgcat cgccagggga ctggatcaaa ggcttcggac gatcctcgat cgccttcaaa
     5101 ccgggtccgc gataaacgag cgccttcatc gtgcgatttt gtgccggacg ccgcgggagt
     5161 tcagttcgcc agcgtacaga gcggagcttg ggcaacgtct cccggctaca cccacttgtc
     5221 ctgagtactg atcaggatgt cgctcggacg tcagcggtgt ccgggtcggc cgaacttgcc
     5281 gtagagcgca gccagcttct gcaccgcttc ttcggactcc cgccgatccg gcgccagcga
     5341 gtcgtcgttg tagccacgca tgtctccgcc cgagaccgcc tgcaccacga tgccatcgct
     5401 gcgcggcacg acattcactc cgtcatacgc gagcgcatag tcgaactcgc gctgcggaat
     5461 cagataggca tgctgaccgc gcaccggcac gatgctctcg tcacgccaca aagcccgcgc
     5521 gccatagcca gtgcagttga tgacgacctt ctcgggaagc gccgccatgt cctgcggctc
     5581 atggaactcg cgatgttcga tgcgtccgcc ggcaaggcga aactgcgtca tcagcgtctg
     5641 cccgtagctg gcgagattga acatgagaac gccgcgccgc cggacgctgg ccgcgcgaaa
     5701 cggcgtgtcc gcgggacgca gattttccga cggcgggaac agatcctgca accggttgcc
     5761 gtacgcggcg aaatgcaatt cgccttccac gggaactcgc ggcgccggcg ccgctgaatc
     5821 cgacacggca tagcggtcga tccagcgcac gggatcgccc gccagtccca ggtggccacg
     5881 aaaagtcttg agcgacgtgc gcgccatctc ttcccacagc gctggaaact ccggactcac
     5941 ggcgtcggtc agcgcgatgc gcgaatccgg cgtgaacgat ccgctggcgc gcgccgagaa
     6001 agtctgctgg atcacgtcac gcgcgtagat agtcaccgac ttgcccgctt gctgggcgag
     6061 tagcgcggag gtcaggccca gcgcaccgca tcccacgacc gcgatgcgat cggacgcctg
     6121 cgtcagcgcc ttgttcacgg cgaccgtgct ggaaccccac gacagcgacc acccgctgcc
     6181 gccatgacca tagttgtgca cgacgagcgt gtcgcgaatg cgctcggtat cgagacgcgg
     6241 gccggcggcg cgaaaggggc gcaggcacac catgagatcg aagatgcgat cagtgcgcgc
     6301 gcgcagtggc gcgagagacc gcgcgggatc gttcggcgcg agtcgctctg gcgtggcacc
     6361 accaatcagc gtgggcgtcg cgcagcctgt cagcgcggct ccaccagcgg ccgcgagact
     6421 ctgcaagagt cgacggcggt tgtagcgagt gcctttcatc actggcgctg gtggaaattg
     6481 cggcatggtg cgttccctga atcacttttg agcttccaga tcctggcgca atgctgccac
     6541 ggtctgacgt cagcgtgacg cattccggaa aatacggcag gcaccgggtg gagttcttta
     6601 cgggctgagg gctcgtttcg agcgcgccac cgatgcgagc agttcctcca ctcgttcctc
     6661 ccggtgcgga agcaacgacc ggcatcgctc gagataccgc cgggcctgga ggatgagc
//
LOCUS       DATDEU010000658         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1004371, whole genome shotgun sequence.
ACCESSION   DATDEU010000658 DATDEU010000000
VERSION     DATDEU010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004371"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VIV63_15670"
     CDS             <1..319
                     /locus_tag="VIV63_15670"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6126092.1"
                     /translation="KIYEDEQLLTRARKVGGVFEQAVHALRELPHVIDIRNYGLVAGI
                     ELEPIAGSPGARAFGVYLKCFERGLLIRTTGDIIALSPSLIIEDKHLDELIGVLSQAI
                     RES"
     gene            complement(411..1772)
                     /locus_tag="VIV63_15675"
     CDS             complement(411..1772)
                     /locus_tag="VIV63_15675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY6126093.1"
                     /translation="MKNLSVGSIPGHVAAMSIQMFVGMVVQVAYFVTDLKFVSMLGNE
                     IAAGFSAAGNLWFVVLALTQVLNVGTAAMVSHAVGAKQHERANLVFNQSLMLSVFMGI
                     FVLVMLYAVASPYMHMEAADAVVAQAGIDYLMGVTPGLAIQFLMISLFATLRGTGIVK
                     PTMIIQFITVGLNILFAAVLTIGWLTGKPYGAFGAGLATTFATAIGVIISLYYFHRHE
                     KYVTFHREQTRPNAEVLKRLFFIGLPIGLEFFFMALVMGVMYWVIRGFGADAQAGLAE
                     GQGAMRFIMLPAMAVAFAAAPIAGQNFGARMPARVRETFNWTVGMSIGIMLALSVFCQ
                     FGSHLLMHIFTYDEEVVAVGSTMLMITSWSFAANGIIFSCSSMFQGLGNTWPTISSSI
                     IRIFVFIVPVLWLSTQPHFDLVHVWYLSVCSMFLQAAVSFALLRREFRRKLDNMTPAL
                     EPA"
     gene            complement(1880..>2559)
                     /locus_tag="VIV63_15680"
     CDS             complement(1880..>2559)
                     /locus_tag="VIV63_15680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY6126094.1"
                     /translation="ALVTGLLAISGVSQAQFSSTWTAVSDYDCRGVSQRGKDPALQAS
                     ADYAFSNGLSIGAWASNIDFGNDEDIEVDLYLNYAGKINDTFSWTAGGTLYMYPTGDA
                     QDDYPEIYVGFNAGAFSFKQWYSWDFGALPDSVDALYSEANYTVPIGESFSLAFHAGY
                     AYGDYWDGFEQFDYAVQGNFTAGKFTIFGKFTGTDASGDFKVEDDVFNNEPRFLVGVM
                     TTFPWGD"
BASE COUNT          561 a          777 c          775 g          446 t
ORIGIN      
        1 gaagatctac gaagacgagc aactgctgac gcgcgcgcga aaggtcggtg gggttttcga
       61 gcaggccgtg catgcgctgc gcgaactgcc gcatgtcatc gacatccgca actacggcct
      121 ggtcgcgggc atagagctcg agccgatcgc cggttcaccg ggcgcacgcg ccttcggcgt
      181 ctacctcaag tgcttcgagc gcgggctgct gatccgcacc acgggcgaca tcatcgcgct
      241 gtcaccttcg ctcatcatcg aggacaaaca tctcgacgaa ctgatcggcg tgctgtcgca
      301 ggcgatccgc gaaagttagg gaaatgggga cattcctgat ttgctagcaa aaggggacag
      361 ggctcgatcg agccgcgtcc ctttttgcta gcaaatcagg aatgtcccca ttacgccggc
      421 tcgagcgccg gcgtcatgtt gtcgagcttg cggcgaaact cgcggcgtaa cagcgcgaag
      481 ctcacggcgg cctgcaggaa catgctgcag accgacaggt accacacgtg cacgagatcg
      541 aagtgcggct gcgtcgagag ccacaacacc ggcacgatga acacgaatat gcggatgatg
      601 ctcgagctga tggtcggcca ggtgttgccg agtccctgga acatcgacga gcagctgaag
      661 atgatgccgt tcgccgcgaa gctccatgac gtgatcatga gcatggtcga gccgacggcc
      721 accacctctt cgtcgtacgt gaagatgtgc atcagcaggt gtgaaccgaa ctgacagaag
      781 acactgagcg ccagcatgat tccgatgctc atgccgaccg tccagttgaa cgtctcccgc
      841 acccgcgcag gcatgcgcgc accgaaattc tgaccggcga tcggcgctgc cgcgaaagcc
      901 acggccatgg cgggcaacat gatgaaacgc atcgcgccct gcccctcggc gagcccggcc
      961 tgcgcatccg cgccaaaacc gcggatgacc cagtacatga cgcccatgac gagcgccatg
     1021 aagaagaact cgagcccgat gggcaggccg atgaagaaca ggcgcttcag cacttcggca
     1081 ttgggccggg tctgctcgcg atggaacgtc acgtatttct cgtgccggtg gaagtaatac
     1141 aggctgatga tcacgccgat ggcggtcgcg aacgtggtag ctagccccgc gccgaacgcg
     1201 ccgtacggct tccccgtgag ccagccgatg gtgagcaccg ccgcgaacag gatgttcaac
     1261 ccgacggtga tgaactggat gatcatcgtc ggcttcacga ttccggtgcc gcgcaaagtc
     1321 gcgaacaacg agatcatgag gaactggata gcgagcccgg gcgtcacgcc catcagatag
     1381 tcgatgcccg cctgggcgac cacggcatcg gccgcttcca tgtgcatgta cgggctcgcc
     1441 accgcataca gcatcacgag cacgaagatg cccatgaaca cggagagcat cagcgactgg
     1501 ttgaacacga ggttggcgcg ctcatgctgt ttcgcgccga ccgcatgcga aaccatcgcc
     1561 gccgtgccga cattcaggac ctgcgtgagc gccagcacga cgaaccacag gttgccggcg
     1621 gcgctgaagc ccgcggcgat ctcgttgccg agcatggaga cgaacttgag gtcggtgacg
     1681 aagtaagcca cctgcacgac catgccgacg aacatctgga tcgacatcgc cgccacatgt
     1741 ccggggatcg atccaacact cagattcttc atgcgggcaa cttacagagt gacgacgcaa
     1801 aaagaaaagg ccggtctttc gaccggccct ttgcttcagt cagcgtggac cgctgatctt
     1861 gttcttcaag agagacggat caatcacccc acgggaacgt ggtcatgaca cccacgagga
     1921 agcgcggctc gttgttgaag acatcgtctt caaccttgaa gtcgccactc gcatcggtgc
     1981 cggtgaactt gccgaaaatg gtgaacttgc cggccgtgaa gttgccttgc acggcgtagt
     2041 cgaactgctc gaagccatcc cagtaatcgc cataggcgta accggcatgg aaggccagcg
     2101 agaagctctc gccgatcgga acggtgtagt tcgcttcgct gtacagcgca tcgacgctgt
     2161 caggcagagc accgaaatcc cacgagtacc actgcttgaa gctgaaggca ccggcattga
     2221 agccgacgta gatttccgga tagtcgtcct gcgcatcacc ggtcgggtac atgtacagcg
     2281 tgccgccagc cgtccacgag aacgtgtcgt tgatcttgcc ggcgtagttc aggtacagat
     2341 ccacttcgat gtcttcgtca ttgccgaagt cgatgttcga cgcccacgcg ccaatggaga
     2401 gtccattgga gaacgcgtag tcggcgcttg cttgcagggc tggatccttc ccgcgctgag
     2461 aaacaccgcg gcagtcgtag tcgctcacgg cggtccaggt cgagctgaat tgagcttggg
     2521 aaacgccgct gatggccaga aggccagtca cgagcgcca
//
LOCUS       DATDEU010000659         2681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1587853, whole genome shotgun sequence.
ACCESSION   DATDEU010000659 DATDEU010000000
VERSION     DATDEU010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587853"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VIV63_15685"
     CDS             complement(<1..1382)
                     /locus_tag="VIV63_15685"
                     /inference="COORDINATES: protein motif:HMM:NF015054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEY6126095.1"
                     /translation="MRIDLLRGMILCVFAFLTQGAFAHGLVQDPPARNWFCGAVTKPD
                     HVQNGVAQYPVCGNAFFAPGVEATAGYSFMSVLTHTTGRAGVGPRTNVCGFNSETWNG
                     AATVWDQPIDWPTVPMTAGPRNFVWNISWGPHFSDSSDFRYWITRPGFVWQTGRALTF
                     ADFEDAPFCDLPYNDATPNANPNLIPDKANALFTTRCTVPSRSGRHVIYAEWGRTPPT
                     FERFHGCIDASFSGTPPPTVTANIVTTPANVSTFTGSGAIALNGSSSVGSNLSYTWSV
                     DSENPALYSITNPMSAQATLNLGVPQASGNVTVNLLVTSGSSSDTETRTILHQPAVSN
                     QWFDLGVLTPDARTLVVGDRVNVRTVSSTGQDAFWPTTPITITSANTGPTQWPVTLAN
                     AVNALNGNVRIGVLNAQNQVVPAANATSNRVYSLSSANIASAFLQVVIGTVPATPTGL
                     AGVPGNAQVAL"
     gene            complement(1758..>2681)
                     /locus_tag="VIV63_15690"
     CDS             complement(1758..>2681)
                     /locus_tag="VIV63_15690"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY6126096.1"
                     /translation="EAVQLDLLPRVRVLQTSAEAHGQIYVPAANLFMFVAVVLFVVAF
                     GSSSALSAAYGASVVGTMLITTLLGALVAASLWNWPVWRVAALFGLMLLVDLAFVAGN
                     ATKIASGGWVPLALAGLMFAMFITWRDGRLRLRAELEERAVPAKKLAELLKPTAKVPG
                     TAVFLVSHTGFVPTALLRNLEHNKVHHEQIVILHVEIQRVPRTDPLCRVLIEEMIPGV
                     YDVRARFGFMETPDVGAALRNARRQGLKVFSEDSSFFLGWHLVRARPRPGFPGLKSRA
                     FAFLQRRSTQAAEFFHMPTRGVVVLATEIEL"
BASE COUNT          491 a          853 c          880 g          457 t
ORIGIN      
        1 agcgccacct gcgcgttgcc cggaactccc gcgaggcctg tcggcgttgc cggcactgtg
       61 ccgatgacca cctgcaggaa tgcgctggcg atgttggcgc tcgacagcga gtacacgcgg
      121 ttgctggtgg cattcgccgc cggcaccacc tggttctgcg cgttcaacac gccgatgcgt
      181 acgttgccat tgagcgcatt cactgcattc gcgagcgtca ctggccactg cgtgggtccg
      241 gtgttcgcgg atgtgatggt gatcggcgtg gtcggccaga acgcatcctg accggtgctc
      301 gacaccgtgc gcacgttgac gcgatcaccc acgacgagcg tgcgcgcatc cggcgtcagc
      361 acgccgagat cgaaccactg attgctcacg gccggctggt gcaggatcgt gcgcgtctcg
      421 gtgtccgaag aactcccgct ggtcacgagc agattcacgg tgacattgcc cgacgcctgc
      481 ggcacgccga gattcagcgt ggcctgcgcg ctcatcgggt tggtgatgct gtacagcgcc
      541 ggattctcgg aatccacgct ccaggtgtag ctcaggttgc tgccgaccga actgctgccg
      601 ttgagtgcga tcgcgcccga tccggtaaac gtcgacacgt tcgcgggagt cgtcacgatg
      661 ttcgcggtga cggtgggcgg cggcgtgccg gagaacgacg cgtcgataca gccatggaag
      721 cgttcgaagg tcggcggcgt gcggccccac tcggcgtaga tcacgtgacg gccgctgcgc
      781 gacggcaccg tgcagcgtgt ggtgaacagc gcgttggcct tgtcaggaat gaggttcggg
      841 ttggcgttcg gcgtcgcgtc gttgtacgga agatcgcaga acggcgcgtc ctcgaagtcc
      901 gcgaaagtga gcgcgcgtcc cgtctgccat acgaagcccg gcctcgtgat ccagtagcgg
      961 aaatccgagc tgtccgagaa atgcggaccc caggagatgt tccacacgaa attgcgcggg
     1021 cccgcggtca tcgggaccgt cggccagtcg ataggctgat cccacaccgt ggccgcgcca
     1081 ttccaggttt cggaattgaa tccgcacacg ttggtgcgcg ggcctactcc ggcgcggccg
     1141 gtggtgtgcg tgagcacact catgaagcta tagccggcgg tcgcctcgac acccggtgca
     1201 aaaaacgcgt taccgcacac cggatattgc gcgaccccgt tctgcacgtg gtcgggcttg
     1261 gtgacggcac cgcagaacca gttgcgtgcc ggcggatcct gcaccagtcc atgcgcgaac
     1321 gcgccctggg tcaggaaagc gaacacacac agaatcatcc ctcgaagtaa gtcgatcctc
     1381 atgacagctc cctcgtgatc attacgaatc ttcgaatgca tcgatgacgc gtcagatcgg
     1441 gagctagggc gggatctccg tgtgcggagc cgcgtgtcgg gtgcgcggcg aaatctgccg
     1501 cgaaagataa cctgcgtcca tacagtcccc cttgcttgcc cgcactcctg tgcgagcggg
     1561 ggcgatgtta ggcccgtagt tgggccgcgc acaactcggg tgcaccgcat ccgggtagtt
     1621 tggacaacgc tgtcaaggcg gattctgact agccgcgcgg ccggaaaggc caaggtgact
     1681 cgcgggactt ttttatgctg gctgtcggac acgggttggc gtgtagcgac aacgcgcgcc
     1741 agcccaaatt gtcttatcta cagctcgatt tcggtggcga ggaccacgac tccgcgagtc
     1801 ggcatgtgga agaactccgc cgcctgcgta ctgcggcgct gcaggaacgc gaatgcgcgg
     1861 gattttagtc cgggaaaccc cggccgcgga cgcgcccgca ccagatgcca gccgaggaag
     1921 aacgaggaat cttcggaaaa tactttgagt ccctgccgcc gggcgttgcg cagggccgcg
     1981 ccgacatcgg gtgtctccat gaagccaaaa cgcgcgcgca cgtcatacac gccgggaatc
     2041 atctcttcga tcaggacacg gcagagcgga tcggtgcgag gcacacgctg gatttccacg
     2101 tgcaggatca cgatctgttc gtggtgaacc ttgttgtgtt cgaggttgcg cagcaatgcc
     2161 gtgggcacga atccggtgtg gctcaccagg aagaccgcgg tacccggcac cttcgcggtg
     2221 ggcttcagca gctcggcgag cttcttcgcc ggcaccgcgc gctcttcgag ctccgcgcgc
     2281 aaacgcaacc ggccgtcacg ccacgtgatg aacatggcga acatcaggcc cgccagcgcc
     2341 agcggcaccc agccaccact cgcgatcttg gtggcgttgc ccgcgacgaa cgccaggtcg
     2401 accagcagca tgagcccgaa cagcgccgcc acgcgccaca ccggccagtt ccacagggat
     2461 gcggcgacca gggcgcctaa caacgtggtg atgagcatcg tgccgaccac cgaggcaccg
     2521 taggcggccg acagcgcgct cgagcttccg aacgcgacga cgaacaggac cacagcgacg
     2581 aacatgaaca ggttcgcggc cggcacgtag atctgcccgt gcgcctcggc cgaggtctgc
     2641 agcacgcgga cgcgcggcag caggtcgagc tgcaccgctt c
//
LOCUS       DATDEU010000660         8393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_568787, whole genome shotgun sequence.
ACCESSION   DATDEU010000660 DATDEU010000000
VERSION     DATDEU010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568787"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VIV63_15695"
     CDS             complement(<1..1130)
                     /locus_tag="VIV63_15695"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEY6126097.1"
                     /translation="MNANPVYPGYDAQRVWRDFPILARQVHGKPLVFLDSAASSQRPA
                     SVIAAVDDYERQHHANVHRGVHQLSQEATQMFEHARERVRTFVNAASTREVIFTRGTT
                     ESINLVAQSYARPRLKRGDEILLTGLEHHANIVPWQLVAEQCGAVVKAVPMDRRGVID
                     AASVAAHMTDRTRIVACAHVSNALGTVLPVREITRLAHARGAVVLIDGAQAVPHQRVD
                     LQALDCDFYAFSAHKMYGPTGIGALIGRESLLEAMPPWQGGGDMILTVSFEKTTFNVL
                     PFKFEAGTPNISGAVGMAAAIDYIDSLGIDRIHAHEQQLLEIATEKLARVPGLTIIGT
                     APDKAAVISFTIDGIHPHDLGTILDAEGVAVRTGHHCAQPVM"
     gene            complement(1127..2320)
                     /locus_tag="VIV63_15700"
     CDS             complement(1127..2320)
                     /locus_tag="VIV63_15700"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEY6126098.1"
                     /translation="MISLPTNKDENWRYANLRPLTKANAEAVASPATTNVTLPAPLPG
                     YERWVFVDGRFAANLSAPAKDSCATLLNARDAGEEFSAMLDAAIATEGADFALARVNG
                     ARGDEVLHIAPADGASASIEITFVASAAAAAGTSYPRVQVHAGRNAKLRLVERHFSAG
                     TADSAINAAFDLALRPDATLDHCRLQNVADAASCFDTLIAHVGERASYRLRTITLGGL
                     ASRSTIVVKLAGRAARCELTAGSIANGIQTHDVFAEIEHSNADTVTRELYRGIATDRG
                     KLGFNGKMVVRESAPGSDSDQSLKTLLTGKGAEASVRPQLEIYTDKVRAKHGATTGKL
                     DEQMLFYLLSRGIDRRTAQTLLQWAFIEDAISRMDNAPLRAEIELLVAAQLNEVSALE
                     GLLGQ"
     gene            complement(2317..3072)
                     /gene="sufC"
                     /locus_tag="VIV63_15705"
     CDS             complement(2317..3072)
                     /gene="sufC"
                     /locus_tag="VIV63_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY6126099.1"
                     /translation="MTALLEISDLSAKVGGKNVLQGLSLTLNAGEVHAIMGPNGSGKS
                     TLAYVLAGRPGYEITGGSVKFQGRDLLSMEAEERARAGMFLGFQYPVEIPGVNNVYLL
                     KAAVNAKRKAAGQPEVDAYEFLQLVRGKMKLMQMDESFLSRGVNEGFSGGEKKRNEIL
                     QMLVLEPALAVLDETDSGLDIDALKVVARGVNSLRAADRGVLLITHYQRLLDHIVPDQ
                     VHVLARGKIARSGDKTLAHELEKRGYDWVLEVA"
     gene            complement(3069..4511)
                     /gene="sufB"
                     /locus_tag="VIV63_15710"
     CDS             complement(3069..4511)
                     /gene="sufB"
                     /locus_tag="VIV63_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY6126100.1"
                     /translation="MSSEPLDALLGKSYRHGFVTDIDSDTLPPGLDEDVVRFISRKKR
                     EPQFMTDWRLKAYRHWLTMREPHWAKLRVNKIDYQSISYFSAPRRDPNAPKSLEEVDP
                     KLLATYEKLGVPLHERARLAGVAVDAVFDSVSVATTFKERLSEAGVIFCSFSDAVQNH
                     PALIEKYLGTVVPQGDNFFAALNSAVFSDGSFVYVPPGVRCPMELSTYFRINAKNTGQ
                     FERTLIIAAPGSHVSYLEGCTAPMRDENQLHAAVVELIAEDDAQIKYSTVQNWYPGDE
                     KGVGGIYNFVTKRGECRGRNSKISWTQVETGSAITWKYPSCVLRGENSVGEFYSVATA
                     NNYQQADTGTKMIHIGANTRSTIVSKGISAGHGQNSYRGLVKILPSAEGARNFTQCDS
                     LLMGGNCGAHTFPYIETRNPSATVEHEASTSKISEDQLFYCLQRGIEEEDAVNMIVSG
                     FCKSVLKELPMEFAVEAQNLLAVSLEGAVG"
     gene            complement(4508..4981)
                     /locus_tag="VIV63_15715"
     CDS             complement(4508..4981)
                     /locus_tag="VIV63_15715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEY6126101.1"
                     /translation="MLRIGKLTDYATVILATLAADRTRLQNASSLAERTHIAAPTVSK
                     LLKQLHRAGLVVSTRGTHGGYQLARDPEDISAATILDALEGPIALTECSSASSHCGIE
                     HTCSTGRAWQRLNVAIRRSLNEISLAQLAGMDTRAPTMPIERELKQLIRPSKVPA"
     gene            5119..5967
                     /locus_tag="VIV63_15720"
     CDS             5119..5967
                     /locus_tag="VIV63_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6126102.1"
                     /translation="MTNSVTNDANGSPAPAGDSVIEVRDVHYSIGGRKIFDGLDVTAR
                     RGRITAFMGPSGTGKTTLLRLITGQSLPERGSVKAWGEEVPRMSGKELFALRRRMGML
                     FQNGALLTDEDVYENVAFPLRAHTKLPESLVRQLVLTKLQAVGLRGAARLMPAELSGG
                     MARRVALARAIVMDPELLIYDEPFVGLDPISMGVICRLIKQLNDALGITSIVVSHDVH
                     ELSTIADDSFLLSGGRVVAQGTPAELHKSNLEEVRQFMGGLADGPVAFHYPAPDLAQD
                     LLEGEA"
     gene            5964..6734
                     /gene="mlaE"
                     /locus_tag="VIV63_15725"
     CDS             5964..6734
                     /gene="mlaE"
                     /locus_tag="VIV63_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEY6126103.1"
                     /translation="MNAQVDSLLVAWLRRIGSMSLFFAQVCARIVPSFAKPGLIVRQI
                     YNAGARSLVIVMLSGLFVGMVLGLLGFDTLQRFGSEDSLGVLAALGMLKVLGPVLTAL
                     LFAGRAGTSLTSEIGLMKSTDQLTAMQMMAVDPLRHVIAPRFIGGILAMPLLAATFSV
                     IGLFGAQLIGVQQMGVDVGAFWSQTQNAVDLRDVTEGIVKSLVFGVACSLIAVYEGYN
                     SEPTPEGVGLATTRTVVTSSVMVLLLNYLLTAAFLTKN"
     gene            6747..7262
                     /gene="mlaD"
                     /locus_tag="VIV63_15730"
     CDS             6747..7262
                     /gene="mlaD"
                     /locus_tag="VIV63_15730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY6126104.1"
                     /translation="MKNSRSLEIGTGLFVLLGFAALAFLTTQLPGTSLKFGGSEGGYT
                     VTARFDNIGDLKPGSPVTMAGVRVGRVESIRFDPKDFKAAVVLRIEDQYKEIPEDSDA
                     SIQTAGLLGGKYVGIGPGGSETFLKQGGQIEFTQSAIVLESLVNKFFANMASKGPDEQ
                     KSEAPAAQEQK"
     gene            7262..7930
                     /locus_tag="VIV63_15735"
     CDS             7262..7930
                     /locus_tag="VIV63_15735"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6126105.1"
                     /translation="MQSLKRIVQAGFISGLLVAGGAHAADAPKATPTSVDTSGPSQLI
                     ESSANILLTGIDARRAEFRKDPTGLYELVASTLLPHFDTPYAAQLVLGPHWRNATPEQ
                     RKRFVDAFYQSLLYTYGDAMVDFTGDRLRVLPTKVAPTDERATVRTEIKRSNGTKVAV
                     NYSLRKVNGAWKAWDVIIDGISYVKSYREDYGAEVQQKGLEAVIARLEEKAQSAKSAK
                     VKTT"
     gene            7934..8263
                     /locus_tag="VIV63_15740"
     CDS             7934..8263
                     /locus_tag="VIV63_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6126106.1"
                     /translation="MSGEARQNVRVGISEPASGRVTVTGELTFASAREARQLGLLVLD
                     GSRADRIIIDCGGITRADSAGLAVLLDWLAWGRRKSRPISLENIPAPLVAIAKISEVD
                     GLLLAAG"
BASE COUNT         1378 a         2627 c         2810 g         1578 t
ORIGIN      
        1 tccatgaccg gttgggcgca atggtggccc gtgcgcacgg ccacgccttc ggcatcgagg
       61 atggtgccga gatcgtgagg atggataccg tcgatggtga aggagatgac cgcggccttg
      121 tcgggcgccg tgccgatgat cgtcaggccg ggaacgcgtg cgagtttttc cgtggcgatc
      181 tcgagcaact gctgctcgtg cgcatggatg cgatcgatgc ccagcgagtc gatgtagtcg
      241 atcgcggccg ccatgcccac ggcgcccgag atgttcggcg tgccggcctc gaacttgaac
      301 ggcaacacgt tgaacgtggt cttctcgaaa ctcacggtga ggatcatgtc gcctccgccc
      361 tgccatggcg gcatggcttc gagcagcgac tcgcggccaa tcagcgcgcc gatgccggtg
      421 ggcccgtaca tcttgtgcgc ggagaaagcg tagaagtcgc agtcgagcgc ctgcaggtcc
      481 acgcgctggt gcggcacggc ctgggcgccg tcgattagca ccacggcgcc gcgcgcatgc
      541 gccaggcgcg tgatctcgcg caccggcagc acggtgccga gcgcattcga cacgtgcgcg
      601 caggcgacga tgcgcgtgcg atcggtcatg tgcgccgcga cgctggctgc gtcgatgaca
      661 ccgcggcggt ccatgggcac ggccttgacg accgcgccgc actgttccgc gaccagttgc
      721 cacggaacga tgttggcgtg gtgttcgagc ccggtgagca ggatctcgtc gccgcgcttc
      781 aggcgcgggc gggcatagga ctgcgccacg agattgatgg attcggtggt gccgcgcgtg
      841 aagatgactt cgcgcgtgga ggcggcgttg acgaaagtgc gcacgcgctc gcgcgcatgc
      901 tcgaacatct gcgtcgcttc ctggctgagc tggtgcacgc cgcgatgcac gttggcgtgg
      961 tgctgtctct cgtagtcgtc cacggccgcg atgacgctgg cggggcgctg cgaggaggcg
     1021 gcgctgtcga ggaacacgag cggctttccg tgcacctgtc gcgcgaggat ggggaagtcg
     1081 cgccacacgc gttgtgcgtc gtagcccggg tagacgggat ttgcgttcat tgccctaata
     1141 acccttcgag tgcggacacc tcgttgagtt gcgccgcgac cagtagttcg atttcggcgc
     1201 gcagcggagc gttgtccatg cgcgagatgg cgtcttcgat gaaggcccac tgcagcaacg
     1261 tctgcgcggt gcgccggtcg atgccgcgcg agagcaggta gaacaacatc tgctcatcga
     1321 gcttgccggt ggtggcgcca tgtttggcgc gcaccttgtc ggtgtagatt tccagttgcg
     1381 gacggaccga ggcctcggcg ccttttcccg tgagcagcgt cttgagtgac tggtcggaat
     1441 cggaccccgg cgcggattcg cgcaccacca tcttgccgtt gaagccgagc ttgccgcggt
     1501 cggtggcgat gccgcgatag agctcgcgcg tgaccgtgtc ggcgttggag tgttcgatct
     1561 cggcgaacac gtcatgggtc tggatgccat tggcgatgct gccggcggtc agttcgcagc
     1621 gagccgcgcg gccggcgagc ttgaccacga tcgtggagcg ggacgcgagg ccgcccagcg
     1681 tgatggtgcg cagccgatag ctggcccgtt cgcccacgtg cgcgatcagc gtgtcgaagc
     1741 agctcgcggc gtcggcgacg ttctgcagcc ggcagtgatc gagggtcgca tcgggacgca
     1801 gggccaggtc gaacgcggcg ttgatggccg agtcggcggt gcccgcgctg aagtggcgct
     1861 cgacgaggcg caatttcgca ttgcgccccg cgtgcacctg cacgcgcggg tacgacgtac
     1921 cggccgccgc ggcggcgctg gcgacgaaag tgatttcgat gctggcgctg gcgccatcgg
     1981 cgggcgcgat gtgcaggact tcgtctccac gcgcgccgtt cacccgcgcc agcgcgaagt
     2041 cggcgccttc ggtggcgatg gccgcgtcga gcatcgccga gaattcctca ccggcgtccc
     2101 gcgcattcag cagcgtggcg catgagtcct tcgcgggcgc cgacagattc gcggcgaatc
     2161 gcccgtcgac gaatacccat cgctcgtagc cgggtaatgg agcgggcagg gtgacgttgg
     2221 tggtcgccgg actcgcgacc gcttccgcgt tcgccttggt cagcggacgc agattggcgt
     2281 atcgccagtt ctcgtccttg ttggtgggca gcgagatcat gcgacctcga gcacccagtc
     2341 gtaaccgcgt ttctcgagct cgtgggccag cgtcttgtcg cccgagcgcg cgatcttgcc
     2401 gcgcgccagc acgtgcacct ggtcgggaac gatgtgatcc agcaggcgct ggtagtgcgt
     2461 gatgagcaac acgccgcgat ccgccgcccg aagcgagttc acgccgcgcg ccaccacctt
     2521 caatgcgtcg atgtcgaggc ccgagtccgt ttcgtcgagc accgcgagcg cgggctcgag
     2581 caccagcatc tgcaggatct cgttgcgctt cttctcgcca ccggaaaatc cttcgttgac
     2641 gccgcgcgac agaaagctct cgtccatctg catgagcttc atcttgccgc gcacgagctg
     2701 caggaattcg tacgcgtcca cttcgggctg gcccgccgcc ttgcgcttgg cattcaccgc
     2761 ggccttcaac agatagacgt tgttgacgcc gggaatttcc accgggtact ggaacccgag
     2821 gaacatgccg gcgcgcgcgc gttcttccgc ttccatcgac aggaggtcgc ggccctggaa
     2881 tttcacgctg ccgccggtga tctcgtaacc cggccggccg gcgagcacgt aggcgagcgt
     2941 gctcttgccg gagccgttgg gacccatgat ggcgtggact tcacccgcgt tcagcgtcag
     3001 cgacagtccc tgcaacacgt tcttgccgcc gaccttggcg gacagatctg aaatttcgag
     3061 gagcgcggtc atccgacggc tccttccagg ctcacggcta acaagttctg cgcctccacg
     3121 gcaaattcca tcggtagttc cttcagtacg ctcttgcaga agccgctcac gatcatgttg
     3181 acggcgtctt cttcctctat gccacgctgc aggcagtaga agagctggtc ttcgctgatc
     3241 ttcgaggtcg aggcttcgtg ttcgaccgtt gcgctggggt tgcgcgtttc gatgtaaggg
     3301 aaggtgtgcg cgccacagtt gccgcccatg agcaacgaat cgcactgcgt gaaattgcgc
     3361 gcgccttcgg ccgacggcag gatcttcacg agcccgcgat aactgttctg cccgtgtccg
     3421 gcggagatgc ccttggacac gatggtgctg cgcgtgttgg cgccgatgtg aatcatcttg
     3481 gtgccggtgt cggcctgctg ataattgttg gccgtcgcga ccgagtagaa ctcacctacc
     3541 gagttctcgc cgcgcagaac gcaagaagga tatttccagg tgatggccga gccggtttcg
     3601 acctgggtcc acgagatctt ggagttgcgg ccacggcact cgccgcgctt ggtgacgaag
     3661 ttgtagatgc cgccgacgcc tttctcgtcg cccggatacc agttctgcac cgtggagtat
     3721 ttgatctgcg cgtcgtcttc ggcgatgagt tccaccaccg cggcgtgaag ctggttctcg
     3781 tcgcgcatcg gcgccgtgca gccttcgagg tagctcacgt gcgagccggg ggcggcgatg
     3841 atcagcgtgc gctcgaactg cccggtgttc ttcgcgttga tgcggaagta cgtcgacagc
     3901 tccatgggac agcgcacgcc gggcggcacg tacacgaacg agccgtcgga gaacaccgcg
     3961 gaattcagcg ccgcgaagaa gttgtcgccc tgcggcacga cagtgcccag gtatttctcg
     4021 atgagcgcgg ggtgattctg cacggcgtcg gagaacgagc agaagatgac gccggcctcg
     4081 gacaggcgct ccttgaacgt cgtcgccacc gacacgctgt cgaataccgc gtccacggcg
     4141 acgccggcga gccgtgcgcg ttcgtgcagc ggtacaccta acttctcgta ggtggcgagc
     4201 agcttggggt cgacttcctc gaggctcttc ggcgcgttcg gatcgcgcct gggcgccgag
     4261 aaataggaga tggactggta gtcgatcttg ttcacgcgca gcttggccca gtgcggctcg
     4321 cgcatggtca gccaatggcg gtaggccttg aggcgccagt cggtcatgaa ctggggctcg
     4381 cgcttcttgc gcgagatgaa acgcacgacg tcttcgtcga gtcccggcgg cagcgtgtcc
     4441 gagtcgatgt cggtgacgaa tccgtgccgg tacgacttgc ccagcagtgc gtctagaggt
     4501 tcggagctca tgccggcacc ttggacggac ggatcagctg cttgagttcg cgttcgatgg
     4561 gcatcgtggg tgcgcgcgtg tccatgccgg cgagttgcgc cagcgagatt tcgttcaggg
     4621 atcgccggat ggcgacgttg agccgttgcc acgcccggcc ggtggagcag gtgtgctcga
     4681 tgccgcagtg gctgctggcc gaggaacatt cggtgagcgc gatgggtcct tccagcgcgt
     4741 ccaggatcgt ggcggcgctg atgtcttccg ggtcgcgcgc gagttgataa ccgccatgcg
     4801 tgccgcgcgt ggagaccacc agccccgcgc ggtgaagctg tttgagcagt ttgctgaccg
     4861 tcggcgccgc gatgtgcgtg cgctcggcca ggctgctggc gttctgcagg cgcgtccgat
     4921 ccgcggccag ggtggccagg atgaccgtcg catagtccgt gagctttccg atccgaagca
     4981 tcttgtgggt actccaggcc ttttttcggg cccgttttaa gtgggaccag tttagtacgg
     5041 attcaatgct tcagaaagcc gggagggggg ccttttgtta tccttcgcgg ccttccagca
     5101 cccggacgac gtgaacgctt gacgaattcg gtgacgaacg acgcgaacgg atcaccggca
     5161 cccgccggcg atagcgttat tgaggtccgt gacgtccact actcgatcgg gggtcgcaag
     5221 atcttcgacg gcctggacgt caccgcgcgc cgcgggcgca tcaccgcgtt catgggtccc
     5281 tcgggcaccg gcaagaccac gctgctgcgg ctcatcaccg gccagtcgct gcccgagcgc
     5341 ggcagcgtca aggcctgggg cgaggaagtc ccgcgcatgt cgggcaagga actcttcgcc
     5401 ctgcgccgcc gcatgggcat gttgttccag aacggcgcgc tgctcaccga cgaagacgtg
     5461 tacgaaaacg tcgcgttccc gctgcgtgcg cacaccaaac taccggagtc gctggttcgc
     5521 cagctcgtgc tgaccaagct ccaggccgtg ggcctgcgtg gcgccgcgcg gctgatgccc
     5581 gcggaactct cgggtggcat ggcgcggcgc gtggccctgg cccgggccat cgtcatggat
     5641 ccggaattgc tgatctacga cgaaccgttc gtggggctcg atcccatttc catgggcgtg
     5701 atctgccggc tcatcaagca gctcaacgat gcgctcggca tcaccagcat cgtggtgtct
     5761 cacgacgtgc acgaactctc gacgatcgcg gacgacagct tcctgctgtc gggcgggcgc
     5821 gtggtggcgc agggcacgcc ggccgaactg cacaagagca acctcgagga ggtccgccag
     5881 ttcatgggcg ggctcgccga cgggccggtc gcgttccatt accccgcgcc tgatctggcg
     5941 caggatctgc tcgaaggaga ggcatgaacg cgcaggtgga ttccttgctc gtcgcctggc
     6001 tgcggcgcat cggcagcatg tcgctgttct tcgcgcaggt gtgcgcgcgg atcgtgccgt
     6061 cgttcgcaaa gcccggcctc atcgtccgcc agatctacaa cgccggtgcg cgctcgctgg
     6121 tcatcgtcat gttgtccggg ctcttcgtcg gcatggtgct gggcctgctc ggtttcgaca
     6181 cgctgcaaag attcggctcg gaagattcgc tcggcgtgct cgcggcgctc ggcatgctca
     6241 aggtgctggg gccggtgttg accgcgcttc tgttcgcggg gcgagcgggc acgtcgctga
     6301 cctcggagat cggtctcatg aagagcaccg atcaactcac cgccatgcag atgatggcgg
     6361 tggatccgct gcgccacgtg attgcgccgc gtttcatcgg cggcatcctc gccatgccgc
     6421 tgctggcggc gacgttcagc gtcattggtc tcttcggcgc gcagctcatc ggcgtgcagc
     6481 agatgggcgt cgacgtcggc gcgttctggt cgcagacgca gaacgccgtc gaccttcgcg
     6541 acgtcaccga aggaatcgtc aagagcctgg tgttcggtgt cgcctgcagc ctcattgcgg
     6601 tctacgaggg ttacaacagc gagccgactc ccgaaggcgt gggcctcgcc accacgcgca
     6661 ccgtggtcac ctcgtcggtg atggtgctgc tgttgaacta tctgctgacc gcggcgttcc
     6721 taacaaagaa ctgatcggaa agattcatga aaaactcacg ctctctcgaa atcggcaccg
     6781 ggttgttcgt cctgctgggt tttgccgcac tggcattcct cacgacgcag ctcccgggca
     6841 cgtcgctcaa gttcggtggc agcgaaggcg gctacaccgt caccgcccgg ttcgacaata
     6901 tcggcgatct gaagccgggt tcgccggtca ccatggcggg tgtgcgcgtc ggacgcgtcg
     6961 agtcaatcag attcgacccc aaagacttca aggcggcggt cgtgttgcgc atcgaggatc
     7021 aatacaagga aatccccgaa gacagcgatg ccagcatcca gacggctggt ttgttgggcg
     7081 gcaaatacgt cggcatcggc cccggcggct ctgaaacctt cctgaagcag ggcggtcaaa
     7141 tcgagttcac acagtcggcc atcgtgctcg agagtctggt caacaagttc ttcgccaaca
     7201 tggcgagcaa aggacctgac gaacagaagt ccgaagcgcc ggccgcgcag gagcagaaat
     7261 gatgcaatct ttgaagcgaa tcgttcaggc tgggttcatc tcgggtttgt tagtcgccgg
     7321 tggcgcgcac gccgctgatg cgcccaaagc cacgccgact tcggtcgaca ccagtggccc
     7381 atcgcagctc atcgagagct cggccaacat cctgttgacg ggcatcgacg cgcgtcgcgc
     7441 ggaattccgc aaggatccga ccgggttgta cgaactcgtg gccagcacgt tgctgccgca
     7501 tttcgacacg ccctacgccg cgcaactcgt gctcggtcct cactggcgca atgcgacgcc
     7561 cgaacagcgc aagcgattcg tcgacgcgtt ctaccagtcg ctgctctaca cctacggcga
     7621 tgccatggtc gacttcaccg gcgatcgtct gagagtgctg cccaccaagg ttgcgccgac
     7681 ggatgaacgc gccacggtgc gcaccgagat caagcgctcg aacggcacca aggtcgcggt
     7741 caactacagc ctgcgcaagg tgaatggcgc gtggaaagcg tgggacgtca tcatcgacgg
     7801 catcagctac gtcaagagct atcgcgaaga ctacggcgcg gaagtgcagc agaagggtct
     7861 cgaggcggtc atcgcgcgcc tagaagagaa ggcgcagagc gcgaagtcgg ccaaggtgaa
     7921 gaccacctga accatgagcg gggaggccag acaaaacgtg cgcgtcggaa tcagtgagcc
     7981 cgcttcgggc cgcgtcacgg tgacgggcga actcacgttc gccagcgcgc gcgaggcgcg
     8041 ccagctcggc ctcctcgtgc tcgatggctc tcgtgccgat cgcatcatca tcgactgcgg
     8101 cggcatcacc cgcgcagaca gcgcggggct cgcggtgctg ctcgactggc tggcctgggg
     8161 ccggcgcaag tcgcgcccga tctcgctcga aaatattcct gcgccactgg tcgccattgc
     8221 gaagatcagc gaggtcgacg gactgttgtt ggccgccggc tgagcagtca acgaacgatt
     8281 gcgcgcgtat ccacgttgcg cacgtcgttc tcaccacaca acgccatgct gatttcgagc
     8341 tcctttcgca tgatcgcgag cgcgagcgcc acgccttcgc cgccccgtgc ggc
//
LOCUS       DATDEU010000661         6449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_568788, whole genome shotgun sequence.
ACCESSION   DATDEU010000661 DATDEU010000000
VERSION     DATDEU010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568788"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VIV63_15745"
     CDS             complement(<1..752)
                     /locus_tag="VIV63_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6126107.1"
                     /translation="MSAVPPVTPPAAPPSDVVNVEVDGKPCQGKKGQMIIHVTDAQNA
                     YVPRFCYHDKLPIAANCRMCLVEVEKAPKPLPACATPIGEGMKIFTKSPKAIGAQKAT
                     MEFLLINHPLDCPICDQGGECELQDLAMGFGRDAARFNERKRVVKDKDIGPLVSTDMT
                     RCIHCTRCVRFGQDIAGIQELGTTGRGEQMEIGTYVERAVEHELSGNIIDLCPVGALN
                     SKPFRYRARSWEMTQAALVSPHDGVGTNLFGHV"
     gene            complement(749..2074)
                     /gene="nuoF"
                     /locus_tag="VIV63_15750"
     CDS             complement(749..2074)
                     /gene="nuoF"
                     /locus_tag="VIV63_15750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY6126108.1"
                     /translation="MAGAADKWADKMNLTVFEPLKFKESWKLENYLKIGGYEAWKKIL
                     AEKPARLAVVDQVKASGLRGRGGAGFPTGTKWSFMPRDATMQKYAVCNSDESEPGTCH
                     DRDILRFNPHSLIEGMAIGGYCMNATVGYNYIRGEFLGEPVPRFEAAVKEAYAAGLLG
                     KNILGSGVDFDLYTAVGAGAYICGEETALLDSLEGKQGKPRFKPPFPAAYGLYGAPTT
                     INNTQSYASVPTILRKGADWFAKLGVANAGGTAIFSVSGHVNKPGNYELPMGIPFAEL
                     LEIAGGVKGGRKLKACIPGGSSVYVVPADKIMAANMDYDSLKAAGSSVGSAAVIVMDE
                     TTCMVKVLERLSRFYKSESCGQCTPCREGTGWMNRVIRRIMAGEGAKSDLQLLVDVAG
                     RIEGHTICALGDAAAWPVQSFIKHFRHEFEYMIDNGGRSIVDDPAKRAA"
     gene            complement(2087..2629)
                     /locus_tag="VIV63_15755"
     CDS             complement(2087..2629)
                     /locus_tag="VIV63_15755"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY6126109.1"
                     /translation="MTHVVKTADGGSFEPVFGEKARVLNEHTRHEIDEWIGRFPTGRQ
                     RSAVLSALRFTQEQNNGFLTPELMDGVAEYLRLPSIQVYEVASFYSMFETHPCGRHHV
                     SICTNISCMLNGAEDLVAHCEKKLGIKLGESTADGRIFLKKEEECLAACTGAPMMMVD
                     HEFHEYLTPEKVDKVLDDLK"
     gene            complement(2626..3879)
                     /locus_tag="VIV63_15760"
     CDS             complement(2626..3879)
                     /locus_tag="VIV63_15760"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY6126110.1"
                     /translation="MNEIRNFTMNFGPQHPAAHGVLRLVLEMDGEVIQKADPHIGLLH
                     RGTEKLAESKPFNQSIGYMDRLDYVSMMCNEHAYVMAIERLLGVTVPIRAQYIRVMFD
                     EVTRVLNHLMWLGAHALDIGAMTVFLLAFKEREDLMDCYEAVSGTRMHATYYRPGGVY
                     RDLPDSMPKYQASKWRTQKNADAMNEARQGSLLEFLDAFVARFPGCVDEYETLLTDNR
                     IWKQRTVNIGVVTPERAMALGFTGAMLRGSGIAWDLRKKQPYEVYADMDFDIPVGVNG
                     DCYDRYLVRIEEMRQSNRIIKQCIDWLKRNPGPVINDDRKVRPPRREEMKGDMESLIH
                     HFKLFTEGYSVPEGETYAAVEAPKGEFGIYLISDGANKPYRLKCRAPGFAHLSALEEM
                     TKGHMLADVVAVIGTQDIVFGEVDR"
     gene            complement(3876..4553)
                     /locus_tag="VIV63_15765"
     CDS             complement(3876..4553)
                     /locus_tag="VIV63_15765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY6126111.1"
                     /translation="MSQQITTLAEKIEARLPGLLLRVTSLPDELCYEVPPEKLKEVAL
                     ILRDSPELKFEILVDVAGIDYLDYGTTEWKTYSATTSGFSRGVNRGGARVPHDGARFA
                     VAYHLLSITHNHRVRLRSRCVDAEDPIIDSLVDIWPGANWFEREAFDLFGILFTGHPD
                     LRRILTDYGFIGHPFRKDFPLIGNVEVRYDADKQRVVYEPVSIEPRTLVPKVIRDDNR
                     YDPALKK"
     gene            complement(4550..5032)
                     /locus_tag="VIV63_15770"
                     /pseudo
     CDS             complement(4550..5032)
                     /locus_tag="VIV63_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979183.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(5065..5433)
                     /locus_tag="VIV63_15775"
     CDS             complement(5065..5433)
                     /locus_tag="VIV63_15775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY6126112.1"
                     /translation="MLANYLPVLIFLTVATGIALVLMTLGLVIGRWQAKSRNDAQKLS
                     PYECGFEAFEDSRMKFDVRYYLVAILFIVFDLEIAFLFPWAVVLDSIGTPGLVAMGIF
                     LFILVVGFIYEWKKGALEWD"
     gene            complement(5565..5649)
                     /locus_tag="VIV63_15780"
     tRNA            complement(5565..5649)
                     /locus_tag="VIV63_15780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5613..5615),aa:Leu,seq:gag)
     gene            complement(5677..6048)
                     /gene="secG"
                     /locus_tag="VIV63_15785"
     CDS             complement(5677..6048)
                     /gene="secG"
                     /locus_tag="VIV63_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY6126113.1"
                     /translation="MLRTILTILQVLSAFAIVGLVLLQRGKGADAGAGFGAGASGTVF
                     GARGASTGLSRMTAIFAAIFMLNSLCLTYLFQRDARAQPTSLLDQVPVNAPATAPAPP
                     PVDANAVPVPAAPVEEAPPKP"
     gene            complement(6064..>6449)
                     /locus_tag="VIV63_15790"
     CDS             complement(6064..>6449)
                     /locus_tag="VIV63_15790"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY6126114.1"
                     /translation="LCVGELLAERESGRTFEVVDRQLDAVLDLSGVAAFSRAVIAYEP
                     VWAIGTGKTASPQQAEEVHAHIRARIAGRDAKIAALVRIQYGGSVKAANAAELFSMPN
                     VDGGLIGGASLKADEFLAIAAAARA"
BASE COUNT         1210 a         1995 c         2016 g         1228 t
ORIGIN      
        1 acgtgtccga aaagattggt gcccacaccg tcgtgcggcg acaccagcgc cgcctgggtc
       61 atttcccacg agcgcgcgcg atagcggaac ggtttggaat tgagcgcgcc caccgggcac
      121 aggtcgatga tgttgccgga gagctcgtgc tccacggcgc gttcgacgta ggtgccgatc
      181 tccatctgct cgccacggcc ggtggtgccg agctcctgga tgccggcaat gtcctggccg
      241 aagcgcacac atcgcgtgca atggatgcag cgcgtcatgt cggtggagac cagcggaccg
      301 atgtccttgt ctttcaccac gcgcttgcgt tcattgaacc ttgccgcgtc gcggccaaag
      361 cccatggcga gatcctgcag ctcgcactcg ccaccctgat cgcagatcgg gcaatcgagc
      421 ggatggttga tgagcaggaa ttccatggtc gctttctgcg cgccaatggc cttcgggctc
      481 ttcgtgaaaa tcttcatgcc ttcgccgatg ggcgtggcgc aggccggcag cggcttcggc
      541 gccttctcca cttccacgag acacatgcgg cagttggccg cgatgggtag tttgtcgtgg
      601 tagcagaagc gcggcacgta cgcgttctgc gcgtccgtga cgtggatgat catctggcct
      661 ttcttgccct ggcagggctt gccgtcgact tcgacgttga cgacatcgga aggcggagct
      721 gccggaggcg tgacgggtgg caccgcgctc atgctgccct cttcgccggg tcgtcgacga
      781 tgctgcggcc accgttgtcg atcatgtatt cgaattcgtg gcggaagtgc ttgatgaaac
      841 tctgcaccgg ccacgcggcg gcatcgccga gcgcgcagat ggtgtggccc tcgatgcggc
      901 ccgcgacatc gaccagcaac tgcaggtcgg acttggctcc ctcgcccgcc atgatccggc
      961 ggatgacgcg attcatccag cccgtgcctt cgcggcacgg cgtgcactga ccgcaggact
     1021 cgctcttgta gaagcgcgac aggcgctcga gcaccttcac catgcaggtg gtctcgtcca
     1081 tgacgatgac ggcggccgaa cccacggacg agcccgcggc cttcagcgag tcgtaatcca
     1141 tgttcgcggc catgatcttg tccgcgggca ccacgtacac cgacgaaccg ccggggatgc
     1201 aggccttgag cttgcgcccg cctttcacgc caccagcgat ctcgagcaac tcggcgaacg
     1261 gaatgcccat gggcagttcg taattgccgg gtttgttgac gtggcccgac accgagaaga
     1321 tggcggtgcc gccggcattg gcgacgccca gcttcgcgaa ccagtccgcg cccttgcgca
     1381 ggatcgtggg aaccgaggcg taactttgcg tgttgttgat cgtcgtcggc gcgccgtaca
     1441 gaccgtaagc agccgggaac ggcggcttga agcgcggctt gccctgcttg ccttcgagcg
     1501 aatcgagcaa cgcggtttct tcaccgcaga tgtaggcgcc agcgcccacg gcggtgtaga
     1561 gatcgaaatc gacacccgag ccgaggatgt tcttgcctaa caaaccggcg gcatacgcct
     1621 ctttgaccgc ggcttcgaaa cgcggcaccg gctcgcccag gaattcgcca cggatatagt
     1681 tgtagcccac cgtggcgttc atgcagtagc caccgatggc catgccttcg atgagcgaat
     1741 gcggattgaa acgcaggatg tcgcgatcgt ggcaggtgcc gggttcggat tcgtccgaat
     1801 tgcacaccgc gtatttctgc atggtggcgt cgcgcggcat gaagctccac ttcgtgccgg
     1861 tggggaaacc cgcgccaccg cggccgcgaa gtcccgaggc cttcacctga tcgacgacgg
     1921 ccagacgcgc gggcttctcg gcgaggattt tcttccacgc ctcgtagcca ccgatcttga
     1981 ggtaattctc gagcttccag gattccttga acttcaacgg ttcgaacacc gtgagattca
     2041 tcttgtcagc ccatttgtcc gcagcgcctg ccatgaatag ctagtcctac ttcaggtcgt
     2101 cgagaacctt gtccaccttc tcgggtgtca ggtactcgtg gaattcgtga tcgaccatca
     2161 tcatcggcgc gcccgtgcag gccgcgaggc actcttcttc cttcttgagg aagatgcggc
     2221 cgtcagcggt gctttcgccg agcttgatgc cgagcttctt ctcgcaatgc gcgacgagat
     2281 cttcggcgcc gttcagcatg cagctgatgt tcgtgcagat gctcacgtga tgccggccgc
     2341 agggatgcgt ctcgaacatc gaatagaagc tcgccacttc gtaaacctgg atcgacggca
     2401 ggcgcaggta ctccgcgacg ccgtccatga gttcgggcgt gaggaagccg ttgttctgtt
     2461 cctgcgtgaa acgcagcgcc gacaacacgg ccgaacgctg gcgtcccgtg ggaaagcgcc
     2521 cgatccactc gtcgatctcg tgacgcgtat gttcgttgag cacgcgcgcc ttctcgccga
     2581 acaccggctc gaagctgcca ccatccgccg tcttcacgac atgcgtcatc gatccacctc
     2641 tccgaacacg atgtcctgcg taccgatcac cgcgaccacg tccgcgagca tgtgaccctt
     2701 ggtcatttcc tcgagcgccg acaggtgcgc gaatccgggc gcgcggcact tcaggcgata
     2761 gggtttgttg gcgccgtcgg aaatcaggta gatgccgaac tcgcccttgg gtgcttcgac
     2821 ggcggcgtag gtttcgcctt cgggcaccga atagccctcc gtgaacagct tgaagtggtg
     2881 aatgagcgat tccatgtcgc ccttcatctc ttcgcggcgc ggcggacgca ccttgcggtc
     2941 gtcattgatg acgggaccgg ggttgcgctt cagccagtcg atgcactgct tgatgatgcg
     3001 gttgctctgg cgcatttctt cgatgcgcac gaggtaacgg tcgtagcagt cgccgttcac
     3061 gcccacaggg atgtcgaaat ccatgtccgc gtaaacctcg taaggctgct tcttgcgcag
     3121 atcccaggcg atccccgagc cgcgcagcat ggcgccggtg aagccgagcg ccatcgcgcg
     3181 ctccggtgtc accacgccga tattcaccgt gcgctgtttc cagatgcggt tgtcggtgag
     3241 cagtgtttcg tattcgtcga cacagcccgg gaagcgcgcg acgaaagcgt cgaggaattc
     3301 gagcagcgaa ccctgacgcg cctcgttcat ggcgtccgcg ttcttctgcg tgcgccactt
     3361 cgaagcctgg tacttcggca tggagtccgg cagatcgcga tacacgccgc ccggacggta
     3421 atacgtggcg tgcatgcgcg tgcccgacac cgcctcataa caatccatga ggtcttcgcg
     3481 ttccttgaac gcgagcagga agacggtcat ggcgccgatg tcgagcgcgt gcgcgccgag
     3541 ccacatgagg tggttcagca cgcgggtgac ttcgtcgaac atcacacgga tgtattgcgc
     3601 gcgaatcgga accgtgacgc ctaacaaccg ctcgatcgcc atgacgtagg cgtgttcgtt
     3661 gcacatcatc gacacgtagt cgagacggtc catgtaaccg atggactggt tgaatggctt
     3721 cgactcggcc agcttctcgg tgccacggtg tagcaggccg atgtgcggat ccgctttctg
     3781 gatgacttcg ccgtccatct cgagcaccag gcgcaacacg ccgtgcgcag ccggatgctg
     3841 cggaccgaag ttcatcgtga agttgcgaat ctcgttcatt tcttcagggc cggatcgtaa
     3901 cggttgtcat cgcggatgac cttgggcacc agcgtgcgcg gctcgatgga cacgggctcg
     3961 tacaccacgc gctgcttgtc ggcgtcgtag cggacttcga cgttgccgat cagcgggaag
     4021 tccttgcgga atggatgacc gatgaatccg tagtccgtga ggatgcggcg cagatccggg
     4081 tggcccgtga acaggatgcc gaacaggtcg aacgcctcgc gctcgaacca gttggcgccg
     4141 ggccagatgt cgacgaggga atcgatgatg ggatcttcgg cgtcgacgca gcgcgaacgc
     4201 aggcgcacac gatggttgtg agtaatcgac agcaggtggt acgcgacggc gaaacgtgcg
     4261 ccgtcatgcg gcacgcgcgc accgccacga ttgacgccgc ggctgaagcc cgatgtcgtg
     4321 gccgaatacg ttttccattc cgtggtgccg tagtcgagat agtcgatgcc ggcgacgtcc
     4381 acgaggattt cgaacttgag ctcaggggag tcgcgcagga tcagggcaac ttccttgagt
     4441 ttctccggcg gcacttcgta gcagagctcg tcgggcagcg aggtaacgcg caagagcaag
     4501 cccggcaggc gggcttcgat tttctcggcg agcgtcgtga tctgttggct catgcgcgct
     4561 ttggaatgtt cgcgatcgag ctggtgcgtt cgatcttctt ctgcagctgg atgattccgt
     4621 agagcagcgc ttcggcggtg ggcggacacc ccggcacgta gacgtctacc gggacgatgc
     4681 gatcgcagcc gcgaaccacg gcataagagt agtggtagta accgccaccg ttggcgcacg
     4741 agcccatgga aatgacccag cgcggctcgg gcatctggtc atagaccttg cgcagtgccg
     4801 gagccatctt gttgaccagc gtaccggcga cgatcatcac gtcggactga cgcgggctcg
     4861 gccggaagac gacgccgaag cggtcgaggt catatcgcga cgcgccggca tgcatcattt
     4921 cgaccgcgca acacgcgagg ccgaaagtca tgggccacag cgaaccggtg cgagcccagt
     4981 tcatgaccgt gtcgacggag gtcgtgacga atccgcgctc ggcgaattca cccgccgccg
     5041 cgttctcgtc gacagcctgg ttctctaatc ccattcgagc gctcccttct tccactcgta
     5101 gatgaatccg accacgagga tgaacaggaa gatgcccatg gcaacgagtc ccggggtgcc
     5161 gatgctgtcg agcaccacgg cccacggaaa gaggaacgca atttcgagat cgaaaacgat
     5221 gaacaggatc gcgacgaggt agtaacgcac gtcgaacttc atgcggctgt cttcgaaagc
     5281 ttcgaagccg cactcataag gagagagttt ctgcgcgtcg ttcctggatt tcgcctgcca
     5341 ccggccgatg acgaggccca gtgtcatcag gacgagcgca atgcccgtcg cgacggtgag
     5401 gaaaataaga acgggcaggt aatttgcgag catctgcgat cccagaacgt cagcccaaaa
     5461 tgactacaaa taccgtcacg aaaaggggca gcaacctctc gacgaatgag agacaaccgc
     5521 ccccggaaat tcgggcgcga ttgtaacagg gataaggaaa gtgatggtgc cgacggggag
     5581 actcgaactc ccacggatcg ctccgctagc ccctcaagct agtgtgtcta ccaattccac
     5641 cacgtcggct ttgttcaaat cttcagcgtg ccttcttcaa ggctttggcg gcgcctcttc
     5701 caccggcgcc gcgggcacag ggaccgcgtt cgcatcaaca ggcggcggcg cgggcgcagt
     5761 cgcaggagca ttgaccggca cctggtcgag cagcgatgtc ggctgcgcgc gggcgtctcg
     5821 ctgaaagaga taagtgaggc acaggctgtt gagcatgaaa atcgcggcga atatcgccgt
     5881 catgcgcgag aggcccgtgc tcgcgccacg cgcaccgaac accgtgcccg aagctccggc
     5941 gccgaatccc gcaccggcat ccgcgccttt gcctcgctgc aagagcacga gaccgacaat
     6001 ggcgaatgcc gaaaggacct gcaggattgt gagaatcgtt cgcaacatct ttgtgtacct
     6061 aaatcaggcg cgagccgcgg cggcaatcgc gaggaactcg tcggctttga gtgatgcgcc
     6121 accgatgagc ccgccgtcca cgttcggcat cgaaaagagc tccgcggcat tcgcggcctt
     6181 cacgctgcca ccgtactgga tgcgcacgag ggcggcgatc ttagcatcgc gacccgcgat
     6241 ccgcgcgcgg atgtgggcat gtacttcctc ggcctgctgc gggctcgccg tcttgccggt
     6301 accgatggcc catacgggct cgtaggcgat gacggcgcgc gaaaacgcgg caacaccgga
     6361 cagatcgaga acggcgtcca gctgacggtc gacgacttcg aacgtccggc ccgactcgcg
     6421 ttccgccagc aactcgccca cgcacagga
//
LOCUS       DATDEU010000662         4273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2025662, whole genome shotgun sequence.
ACCESSION   DATDEU010000662 DATDEU010000000
VERSION     DATDEU010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025662"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /gene="mmsA"
                     /locus_tag="VIV63_15795"
     CDS             <1..1011
                     /gene="mmsA"
                     /locus_tag="VIV63_15795"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY6126115.1"
                     /translation="VALACGNCFILKPSERDPGPGLLLAQWLKEAGLPDGVFNVVNGD
                     KVAVDALLRHPEVNAVSFVGSTPIAEYVQREGIAHGKRVQALGGAKNHLVMMPDADET
                     QVCDALLGAAYGSAGERCMAISVAVAVGDATADRLVGALAERVRTLRVDQSQNGAADM
                     GPLITAAHRAKVVDYIAAGEAEGARLVVDGRGLKVPGHENGFFLGGSLFDGVSSSMKI
                     YREEIFGPVLVVVRVATAADAIKLVNSHEFANGTAVFTRDGGAARAFVQGIEVGMVGV
                     NVPIPVPMAFFSFGGWRRSIFSDHAMHGMEGVRFYTRLKTVTSRWPEGKGSAEFVMPT
                     MK"
     gene            1031..1588
                     /locus_tag="VIV63_15800"
     CDS             1031..1588
                     /locus_tag="VIV63_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126116.1"
                     /translation="MQVENPQLGFPSSFCCNCGVAECSTETQDTRVSRFFGIGRTDTT
                     FKLSVPVCTACRRTIRRKPAGFFSRLGVLVLVTGVIFGICIAMGTTVALPLWIDYMYA
                     GSVVLGLLLTFLFYRLRRPKPPQTSFYQPVRIKNVRLQFSGLMAGEGQVAFMKLAFTN
                     PDYLSAFAVANQDAIKAKRVAVVKA"
     gene            1683..1820
                     /locus_tag="VIV63_15805"
     CDS             1683..1820
                     /locus_tag="VIV63_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126117.1"
                     /translation="MDAPEPNCRPLFRRLSLAWLAVLIALGLWQGYGTWLLGGGASRA
                     F"
     gene            1810..2325
                     /locus_tag="VIV63_15810"
     CDS             1810..2325
                     /locus_tag="VIV63_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126118.1"
                     /translation="MPSEGVLLASMAVFAALIALPALAAASRRRLMREASEVAVALSA
                     LAVVVRQNAACPPVDATLGQRVQRLRAPEVTTLLLAQELPRANPELLADTAQRLALRL
                     KRRVAFERKMLARTASGRRRGAVAASIPPLVLLAMHVGGLRIPTAALLLVLAIETLGC
                     VLLWRVARVEI"
     gene            2326..3069
                     /locus_tag="VIV63_15815"
     CDS             2326..3069
                     /locus_tag="VIV63_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY6126119.1"
                     /translation="MPLPILVALASVLFALSVFGLRGLLGIRHALRCAELNRLDPPEH
                     WQATRAWLGAVAAVPVFFASSGLGGGAWLAALGVAALGFWVAPQFLASARLRVEQELL
                     DDLSVHFDLIALSMEAGSTLPAAIATCAHRAPDGALRRSWARVILEIHAGVDPLEALR
                     ALEQRTGLRPFSALVTALRSAEKFGIPAAQVFRDRARQSAAQRFARAEHLARAAPLRL
                     WAALVLCIAPCTFVVLAFPLAQLLARVLD"
     gene            complement(3066..3704)
                     /locus_tag="VIV63_15820"
     CDS             complement(3066..3704)
                     /locus_tag="VIV63_15820"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY6126120.1"
                     /translation="MTDTGSSEASHIHVVVLAAGSSSRFGSPKQLVRVNGRPLMHTVV
                     SRAVELAGHSVTVVLGANAAELAPLLRHSPAGIAVNRDWEEGIASSIREGLAHAPPSA
                     DGVLIVLADQAAVTTEDLRRLAGVWRRAPASIAVAQYAGNIGVPAIFPRWCFRELNEL
                     RGDRGAQLLLNRHVDRLVRLPMPSAELDIDRPEDLLALEENTQLKRRNIPDA"
     gene            complement(3701..3925)
                     /locus_tag="VIV63_15825"
     CDS             complement(3701..3925)
                     /locus_tag="VIV63_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126121.1"
                     /translation="MNLEPQKTESDIRNNSGVARVLASLTILALGAITILVVLEVIPR
                     SAFTEIAGKTALIAGVCAVSALAIGLLSRR"
     gene            4093..>4273
                     /locus_tag="VIV63_15830"
     CDS             4093..>4273
                     /locus_tag="VIV63_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126122.1"
                     /translation="MSTQFFERQDAQKKYTLWLVAGFIAAILLVVLVVNVVVILGLGI
                     HPMYVLRHEPHVVMWI"
BASE COUNT          626 a         1339 c         1491 g          817 t
ORIGIN      
        1 gtggcgctgg cctgcggcaa ttgcttcatc ctcaagccgt cggagcgcga cccgggcccg
       61 ggattgttgt tagcgcagtg gctcaaggaa gccggactgc ccgacggcgt gttcaacgtg
      121 gtcaatggcg acaaggtggc ggtcgatgcg ctgctgcgcc acccggaagt gaacgccgtc
      181 agcttcgtcg gctcgacgcc gatcgccgag tacgtgcagc gcgaaggcat cgcgcacggc
      241 aaacgcgtgc aggcgctggg cggcgccaag aatcacctgg tgatgatgcc cgatgccgac
      301 gaaacgcagg tctgcgacgc cttgttaggc gcggcctacg gatcggcggg cgagcgctgc
      361 atggcgattt cggtcgcggt cgccgtgggc gacgccaccg cagaccggct ggtgggcgcg
      421 ctcgcggagc gggtgcgcac gttgcgcgtc gatcagtcgc agaacggcgc ggccgacatg
      481 ggtccgctga tcacggcggc gcatcgcgcc aaggtcgtcg actacatcgc ggcgggtgag
      541 gccgaaggcg cgcgcctggt ggtggacggc cgcggcttga aagtgccggg gcacgaaaac
      601 ggtttcttcc tgggcggcag cctgttcgac ggcgtgtcat cgtcgatgaa gatctatcgc
      661 gaggagatct tcgggcccgt gctcgtcgtg gtgcgcgtcg cgaccgcggc ggatgcgatc
      721 aagctcgtga actcgcacga gttcgccaat ggcacggcgg tgttcactcg ggacggcggc
      781 gcggcgcgcg cgttcgtgca gggcatcgaa gtgggaatgg tcggcgtcaa cgttccgatt
      841 cccgtgccca tggcgttctt cagtttcggc ggctggcgcc gctcgatttt ctcggaccac
      901 gccatgcatg gaatggaagg cgtgcgcttc tacacccgat tgaagacggt cacgagccgc
      961 tggcccgaag gaaagggttc cgccgagttc gtcatgccga cgatgaaata agaaataaag
     1021 aggccggttc atgcaggtcg agaatccgca actcggtttt ccgtcgtcgt tctgctgcaa
     1081 ctgcggcgtt gcggaatgct cgaccgaaac ccaggacacg cgcgtgtcgc gcttcttcgg
     1141 catcggccgc acggacacga ccttcaaact atccgtgccg gtctgcaccg cctgccggcg
     1201 gaccatccgt cgcaaacccg cgggtttctt ctcgcggctc ggcgtgctgg tgctggtcac
     1261 cggcgtcatt ttcggaatct gcatcgcgat gggcacgacg gtcgcgctgc cgctgtggat
     1321 cgactacatg tacgcgggca gcgtggtgct gggccttttg ctgacgttcc tgttctatcg
     1381 gctgcgccgc cccaaaccgc cgcagaccag cttctatcaa cccgtgcgca tcaagaatgt
     1441 tcgcctgcag ttctccgggc tcatggccgg ggaagggcag gtggcgttca tgaagctggc
     1501 attcaccaat cccgactatc tgagtgcatt cgcggtggcg aatcaggacg cgatcaaggc
     1561 caaacgcgtc gcggtggtga aagcctagat acgcaaacct cggtgtttgc agtcgattgc
     1621 cgggctgacg cccgcgtggt ttcgcgcttc aatattccgc aacttccggg cggagagcgc
     1681 gaatggatgc gccagaacct aactgtcgac ccctgttccg tcgcctgagt ctcgcgtggc
     1741 tcgcggtgct gatcgcgctc gggttgtggc agggatacgg aacgtggctg ctcggcggcg
     1801 gagcgtctcg tgccttctga aggcgtgttg ttagcctcca tggccgtgtt cgccgcgctc
     1861 atcgcgttgc cggcgctggc cgccgccagc cggcggcgtc tcatgcgtga agccagcgag
     1921 gtcgccgtcg cgttgtccgc cctggcagtc gtggttcgcc agaacgcggc gtgtccgcca
     1981 gtcgacgcca ccctggggca acgcgtgcag cggctgcgcg cgccggaggt caccacgctg
     2041 ctgctggcgc aggagctccc gcgcgccaat ccggagttgc tggccgacac cgcgcaacgc
     2101 ctcgcgctgc ggctcaagcg gcgcgtggcc ttcgaacgca agatgctggc gcgcaccgcg
     2161 tcgggtcggc ggcgcggcgc cgtcgcggcg tccattccgc cgctggtgct gctcgccatg
     2221 cacgtcggcg ggttgcgcat cccgaccgcc gcgctgctgc tcgtgctggc catcgaaaca
     2281 ctgggttgcg tgttgctctg gcgagtcgcg cgcgtggaaa tctgaatgcc gttgcccatt
     2341 ctcgtcgcgc tcgcgtcggt gctgttcgcg ctcagcgttt tcggattgcg cggattgctc
     2401 ggcatacgcc atgcgctgcg ctgcgcggag ctcaaccggc tcgatccgcc ggagcactgg
     2461 caggcgacgc gcgcatggct gggggcggtt gccgcggtgc cggtgttttt tgcgtcgtcg
     2521 gggctcggag gcggtgcgtg gctcgcggct ctgggtgttg cggcgctggg gttctgggtg
     2581 gcgccacagt tcctcgcgtc ggcacgtctg cgcgtcgaac aggagttact cgatgacctg
     2641 tccgtgcact tcgatctcat cgcgctctcg atggaggcgg gttcgacttt gccggccgcc
     2701 atcgccacct gcgcgcatcg cgcgccggac ggcgcgcttc gcagatcgtg ggcgcgcgtc
     2761 attctcgaaa ttcacgcggg tgtcgacccg ctcgaggcgc tgcgtgcgct ggaacaacgc
     2821 accgggctca ggccgttctc ggcgttggtg accgcgctgc gctcggccga aaaattcggc
     2881 ataccggccg cgcaggtgtt ccgcgatcgc gcgcgtcagt ccgccgcgca gcggtttgcg
     2941 cgcgccgaac atcttgcccg cgccgcgccc ctgcggctct gggccgcgct ggtgttgtgt
     3001 atcgctccct gcacgttcgt cgtgctggca tttccgctgg cgcaactgtt ggcgcgcgtt
     3061 ctcgactaag cgtccggaat attgcggcgt ttgagctgcg tgttctcttc cagcgccagc
     3121 aggtcctcgg ggcggtcgat gtcgagttcg gcgctcggca tgggcagccg taccaggcgg
     3181 tcgacgtgcc ggttcagcag tagttgggcg ccgcgatccc cgcgaagctc gttcagctcg
     3241 cgaaaacacc agcgcggaaa gatggccgga acgccgatgt tgcccgcgta ttgggcgacg
     3301 gcgatcgacg ccggtgcgcg tcgccagaca ccggcgagcc ggcgaaggtc ttcagtggtc
     3361 acggcggcct ggtcggcgag aacgatgagc acgccatcgg ctgacggcgg tgcgtgtgca
     3421 aggccttcgc gaatggagct cgcaatgcct tcttcccaat cgcggttcac ggcaataccc
     3481 gcgggactgt ggcggagcag gggcgcgagt tcggcggcgt tggcgccgag caccacggtg
     3541 acggaatggc cggcgagttc caccgcgcgg ctgaccacgg tatgcatcag cgggcgtccg
     3601 ttgacgcgca cgagctgctt gggagatccg aatctcgagg agcttcctgc ggccagcacc
     3661 acgacgtgga tgtggctcgc ctcgctgctc cccgtgtcgg tcaccgccgg gagaggagcc
     3721 cgatggcgag cgcggacacc gcgcagacac cggcgatcag cgcggttttg ccggcgatct
     3781 cggtgaaggc cgaacgcggg atgacctcga gcaccacgag gatcgtgatg gcgcccaatg
     3841 ccaggatggt gaggctcgcg agtacgcggg ccacgccact gttgttgcgg atgtcggact
     3901 cggttttctg cggttcgagg ttcatggcgg gcgatattac tccggttcgg ccgggctact
     3961 gtctggcgtt ggatacaacc gttaagcggg tcgcatttgg cagcaatttt ttgagggcgg
     4021 ccgtgcgcca gagcacggcc tacctattga aatcacggta atttgccgct gtcagttcaa
     4081 ccggttgaag tcgtgagcac acagtttttc gaacggcagg acgcccagaa aaagtacacc
     4141 ctctggctgg tggccgggtt catcgccgcc atcctgctgg tcgtgctggt cgtgaacgtg
     4201 gtggtcattc ttggccttgg catccacccg atgtacgtgc ttcgccacga gccccacgtg
     4261 gtcatgtgga tca
//
LOCUS       DATDEU010000663        13220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2171439, whole genome shotgun sequence.
ACCESSION   DATDEU010000663 DATDEU010000000
VERSION     DATDEU010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171439"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VIV63_15835"
     CDS             <1..479
                     /locus_tag="VIV63_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY6126123.1"
                     /translation="GARMPANGPPYLPQDRIDLVRRWIAAGAPVSSAPADRLIVTSTI
                     PAVSETAAPGLGKLTVIFNAPVDSSRVSADTFTLRDASDQPVALAAVRVPAGRQNVVE
                     ITTAQSLAAGSYQLSVLGDGPAPLADEAGHVLDGDADGTPGGDTLIPFDVNAGAAR"
     gene            476..1570
                     /locus_tag="VIV63_15840"
     CDS             476..1570
                     /locus_tag="VIV63_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126124.1"
                     /translation="MIRSNSFIRLLGVVALITFAGAANAEPYLAAQMGLKCVQCHVNP
                     TGGGLRSVFGNTFAQTQLAAKKIGSDEDLWSGQVMKFLSVGGNARANFSYSDVPNQSA
                     TNDFAVEEARAYLDFGVIPNRLSVYIDQRFAPGNATNLEANIRYWISENSLYVKAGRM
                     YLPFGYRFEDDNAFVRQLSGINMQAPDEGIEFGLEKGQWSTQFALSNGTAGAPESDDG
                     KQFTLRTEYVGSAWRLGASGVVNDTEAGDRFGAGVFGAWRLGSVTLIGEVDYFDDDSI
                     GVAGRKLMASLVEADWKLRQGHNVKFTYEWLEPDDDVDEDEQTRTSLLYEWSPIQFVQ
                     LRGGVRHYDGIPQNDLQNRTQAFLQLHGFF"
     gene            1557..2345
                     /locus_tag="VIV63_15845"
     CDS             1557..2345
                     /locus_tag="VIV63_15845"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6126125.1"
                     /translation="MGSSEATAFDGIAQDYDAEFTATALGSVLRDMVWRRYDARFAGC
                     ESLLEIGCGTGEDAIYLARRGHRVLASDASTQMVRIAEAKAERAGVAHRIRFLCAPMG
                     WLGPAIAGESFDGFYSNFGAINCEPRLEESIGTIAPHLAPGAPLVWVVMGRYVPWEWA
                     WFIAHGHARKAFRRLSRDGVSWRGLTIHYPAPATLRRILQPYFDPTGARALGFALPPS
                     FAGAWLDHSPRALAALTRLEQLAQGWSACATLADHYILEAQRKP"
     gene            2342..3166
                     /locus_tag="VIV63_15850"
     CDS             2342..3166
                     /locus_tag="VIV63_15850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6126126.1"
                     /translation="MNAYAPLRDSPVRSYSAKLARFAQFAEPELRGIVAGLRLPPRAV
                     VLDLGCGVGLVSALLAEAIGGSGRVIGADLSLPHLQAAKANYLGPLVQADAARMCFRP
                     GAFDVIWSCNTLNHVDDPVGVLKSLKPLLRGSGRVVLAQSGFLPEMFFAWDAPLDEAV
                     RRACHAYYRNRYSLEVADTAGLRGLVRLLDLAGYRRGAVRTVVIERLQPLGAADAAYF
                     EETMFAGTWGERLKPFLDAREWDALRRYTDPESAEYCLHRRDFHHLQTLTVCEGHG"
     gene            3159..3884
                     /locus_tag="VIV63_15855"
     CDS             3159..3884
                     /locus_tag="VIV63_15855"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY6126127.1"
                     /translation="MAERATGGFTGALLDADRARPLGAAFGRILHLGARLLRHDRYDD
                     YRFERCGDLRLVVLPTVSNPKLLRTGAFFASVLNQQAVAPVTALDLGTGSGICALAVA
                     PRANRVVAVDINRAAVRCAQINASLNGLEHKVEVRPGDLFSSVPGERFDLVMFNPPFL
                     SGAPRDDRDAAWRGAGLAGRFAAELADHLTPGGRALLLLSSWGAACRDHVDELVRRGF
                     RLTAIARKHHVNETITIVEVVPP"
     gene            3881..4783
                     /locus_tag="VIV63_15860"
     CDS             3881..4783
                     /locus_tag="VIV63_15860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6126128.1"
                     /translation="MSPRVSVILPSFNRLEFLRTAVDSVLAQSLSDWELIVIDDGSAV
                     PVPAYLDWIASLARVRVFRRAHCGNPAAVRNAGIREARGDYVAFLDSDDLWAAHKLSA
                     QLEALGRSGLRWGYTAFRHIDAAAAPLVRSRYQAWRPTVYRTLEGVVRLDAMIPLPSV
                     IVERALLESVGGFDERLRYYEDYDLWFRLALQGEADVLATPLVSIRRHDQHYSDVDQA
                     LTRENLDQLFATMLAQLEDENLRGIVRRQRAVNSGRLAGVHASAHDARGMLRALRQSF
                     AHSKVQPLWWAGAARALARCLLPP"
     gene            4780..6528
                     /locus_tag="VIV63_15865"
     CDS             4780..6528
                     /locus_tag="VIV63_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6126129.1"
                     /translation="MNAFRALWTLLPRDLRRRFVGLQLLALVMAFSTVAGLVSIMPFL
                     ALLSDPGVFAASPRLQAAQAALGFSTPRSFLVAIGMVFVLTLFIANAVNYFGARSMYA
                     FALRAADEIRVRLFGAYLRSDYLFHLRNDHARLGNDVLHESDRVAAVIHGGFQLLTAG
                     AVVTLISVSILVMNPAISLGAIVVFGGSYALTYARSRRRLQRNGQELTRESARRATVV
                     RQTFAAIKELLVSHRQEPFQRQFAEATGGVSRAIVRTHTIGMQPKYVFECLAALALVV
                     TALLLTSVEQRMGSWMAQLTFLALCAYRLLPSLQQLFTGLVTVRAHAQVLFTLAQRLA
                     EPATAGAAPARDEAAWRGMPRADIVFDRVSFRYADDSSLAVDQVSLRIRSGQTIGIAG
                     PNGSGKSTLADLLLGLLTPTSGRILVDGADISGRNRIAWQSTAAYLPQHVALIDGTVA
                     VNIALGVPTADIDTARLERAARLACVDEFVAPMEEGYQTRLGESGTQLSGGQRQRIGI
                     ARALYRDASMLVLDEATSSLDTVSEREITAALRQRPPGVTVVLIAHRRHALEDCDVIF
                     EMHDGLIRASEPAACA"
     gene            6519..7523
                     /locus_tag="VIV63_15870"
     CDS             6519..7523
                     /locus_tag="VIV63_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126130.1"
                     /translation="MRVSRLDPFEERPRRTWRRRLTLLGAVVEFECDSPALRAVVDAA
                     YAGIPAPRPRARAPRYFVRIRTATDSATFRGEPPQPRLTSGAGLLCASVDASNYTVVD
                     PASRSALIVASPAMLAHPYYLRCEFVEFALFTLAARDQAVVALHAACVGHGNRGVLLL
                     GQSGAGKSTATLQSMLSGLAVLSEDVVFVEPRRMRATGVPNFLHLRRESLRLLSRPLA
                     RRIRSAPLIRRRSGVIKHEFDLRQFEGAAALQPLQLVATVVLSRRRAKRGAPLLEKLL
                     VEPLLRALDQDQPYARTRAHWHALRRGLGKIPAYQLLRGADPAQAGVELRRLLRGTRA
                     "
     gene            7520..8620
                     /locus_tag="VIV63_15875"
     CDS             7520..8620
                     /locus_tag="VIV63_15875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6126131.1"
                     /translation="MKVALVVPGGVDRPGGTLVIPALMSLVGRLAARHELRVFTLYQE
                     PQPSRWSVLGAHVINVGARQRHARAVAAICSEHRRVRFDLVHAIWAGSNGLAGVAAAT
                     LLRLPSLVHLAGGELVAIGDIGYGGGLRLRNRVANRWALRRASVVTAASAPMLDAARS
                     LGIEAMRVPLGVDLTAWPPRAPRRRDVNAPARLIHVASLNLVKDQGCLLRAFAQLVAE
                     GRDVEMDIVGVDTLHGSVQALAQELGLSSRVHFHGLLGHVQLRGLFERADLLAMASRH
                     EAGPLVTLEAGVIGVPTVGTSVGHIAEWAPHAALASAVGDPAALAANLARLLDDEDLR
                     LRIAAEAQRRALADDADCTARLFETQYHRVVA"
     gene            8617..10584
                     /locus_tag="VIV63_15880"
     CDS             8617..10584
                     /locus_tag="VIV63_15880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY6126132.1"
                     /translation="MSAAHSPVAYRADIDGLRAVAVVAVILCHAGTPYLNGGFAGVDV
                     FFVISGYLITQILVPATAGGEWRPIGEFYLRRVRRILPALFVMFGVVTIAALVLFVPS
                     ELERFGADLRLASVMLGNLAGWGNGNYFDAMTAQLPLLHVWSVAVEEQFYLAYPWLIL
                     AVARWMPGRRTLVLLLCALASLAFCLYCANRQYVANYYLAPTRGWELLAGALLVGVEW
                     PLLARRWLRELLAALSLVALCACFVFLRQDDSWPNLTALLPCLATAGLLAAGRAGPTL
                     SSRMLEWRPVVFTGLISYSLYLWHAPALTFFERFNIVPPSAWQLAALLAAVYAVSALS
                     WAYIEEPVRRRRVLTDSRRLLWVVAATVLFLVVVATLLVRTHGLPQRFPAPLRAMIQG
                     VDLHRDGARCMGLSVAQVTAGELCRFGPVEDRRPRVIAWGDSHAATLMPAFESVALSR
                     GVSLDFAARAACRPLLGVTSELRADRHVPACEQFNEAMTSLVTKLAPDMVILAAHWSE
                     PGDRFTSPALQARSGESIFRAGLERTVQRIASARTQVCIVLDVPELNYPIPHALLTAR
                     QRGLDTEFLRITRTELAARYGSTESDIRAVVAAFHQVVVDPKESLCRGERCDFGNATE
                     VYYMDANHLTTAGARAVTRTIDGCLAALERR"
     gene            complement(10581..11336)
                     /locus_tag="VIV63_15885"
     CDS             complement(10581..11336)
                     /locus_tag="VIV63_15885"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY6126133.1"
                     /translation="MRVNTASLFERLLPLFDAGIVCDVGSMDGTEALRFARRLPHASV
                     FAFEPNPWNLAPMRANLDLQRARVEIVPCAVADRETRSPFHVLRADYANTGPQRGRSS
                     LYPAVVASELLEVIEVPTVRLDAFLATRTAPQARIALWIDAEGMAYETLAGASALAAR
                     LQLVHVEVERVPCMNAAQKLYPDVRRLLATFGLREIASDGDSRHRQFNALFVRDELTL
                     SLRLRIAAWTAIESLSFLFHGALRRLRIAARPN"
     gene            complement(11327..12379)
                     /locus_tag="VIV63_15890"
     CDS             complement(11327..12379)
                     /locus_tag="VIV63_15890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY6126134.1"
                     /translation="MTPRDGEPPVSCKPWRSQESPRLILAMRFQALGDTLITLPYLLS
                     LKRRFPQADLHFLTRDEVCQVPAAVDVFDRVFTFGGGRSRMWQLLSVMGRMPSLLAQR
                     YDLVLDLQNSHISRLVRRLIAPRAWAEFDRFSPRSAGERNRRTIAAAIDCRPALDTGL
                     TLRRDPDVDTLLREHGWKPGHELVVLNPAGYSPARAWPIENYCEFARLWIARHPRTQI
                     VLLLLPSQRDKSRAIAAALGDACVDLTGHADQLQAFAIAGRARLVLSEDSGLMHMSWV
                     QGTPTLALFSTSRKDWSAPQGSWSDCLDSSDLPCGPCGLEVCRFGDNRCLTRYDARLV
                     AERAEMLVRPEISKCG"
     gene            complement(12376..>13220)
                     /locus_tag="VIV63_15895"
     CDS             complement(12376..>13220)
                     /locus_tag="VIV63_15895"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY6126135.1"
                     /translation="SDVDLLVSPRQADRLSATLRSIGYTPTHSCQRHTVFVPADRPVP
                     RRFGEHPDVPFRIELHTTVTENLPVSLIDVTERIWPAGTRSGANAYRDTAALMCHLLL
                     HAAGNMRTNALRYIQLVDIALLARRMDAAQWAQLLGSPAQPARAWWMFPVLALTARLM
                     PHTVPDEVLAGARAISPTLLRVKSQRQALNDVSWSNLRIAALPGFEWSRSPMELARFV
                     RSRLLPPRQAFSELADTVKNQPDLLRIAWYAQPHGMRILRWLTSRPPRVQTLLTVLAG
                     QARP"
BASE COUNT         1953 a         4448 c         4545 g         2274 t
ORIGIN      
        1 tgggcgcacg catgcccgcg aacggaccgc cctatcttcc gcaggatcgc atcgacctgg
       61 tgcgccgctg gattgccgcg ggtgcaccgg tgtcatcggc gcccgcagac cgcctgatcg
      121 tgaccagcac gatcccggcc gtgtcggaaa ccgcggcgcc aggacttgga aaactcaccg
      181 tcatcttcaa cgccccggta gacagttcac gcgtcagcgc tgacaccttc acgctgcgcg
      241 atgcgtcgga tcaacccgtc gcattggccg cggtacgcgt gcccgcgggc cggcagaacg
      301 tggtcgaaat caccacggcg caatcgctcg ctgccggcag ctatcagctc tccgtactcg
      361 gtgacgggcc tgcgccgctc gccgacgagg ccggtcacgt gctcgacggc gatgccgacg
      421 gaacgcccgg cggggacacg ctcattccct tcgatgtcaa cgcaggagcc gcccgatgat
      481 ccgctcgaat agtttcattc gtctgctggg cgtggttgcg ctgatcacct tcgcgggcgc
      541 ggcgaacgcg gaaccctatc tggcggcgca gatgggtttg aagtgcgtgc agtgtcacgt
      601 caatccgacg ggtggcggac tgcgcagcgt gttcggcaac accttcgcgc agacccagct
      661 cgcggcgaag aagatcggca gcgatgaaga tctgtggagc ggccaggtca tgaaattcct
      721 gtcggtgggc ggcaatgcgc gcgccaactt cagttattcg gacgtgccca accagagcgc
      781 caccaacgac ttcgcggtgg aggaagcgcg cgcgtatctc gacttcggcg tgattccgaa
      841 ccggctgtcg gtgtacatcg accagcgctt cgcgccgggc aacgctacca atctcgaagc
      901 caacatccgc tactggatca gcgagaacag cctgtacgtg aaggcaggcc gcatgtacct
      961 gccattcggc taccggttcg aggacgacaa cgcgttcgtg cgtcagctct cgggcatcaa
     1021 catgcaggcg cccgacgagg gcatcgagtt cgggctcgag aagggccagt ggagcacgca
     1081 gttcgcgctc agcaacggaa ccgccggcgc gcccgagtcg gacgacggca agcagttcac
     1141 gctgcgaacg gagtacgtcg gttcggcgtg gcgcctgggg gcgagcggcg tggtcaacga
     1201 cacggaagcg ggcgatcgat tcggcgccgg agtgttcggc gcctggcgac tgggttccgt
     1261 cacgctcatc ggtgaggtcg actacttcga cgacgacagc atcggcgtcg ccggccgcaa
     1321 gctgatggcc tcgctggtcg aagccgactg gaagctgcgc cagggccaca acgtcaagtt
     1381 cacgtacgag tggctcgagc cggatgacga cgtcgacgaa gacgagcaga cgcgcacgag
     1441 cctgctgtac gagtggtcgc ctatccagtt tgtccagttg cgaggcggag tccggcacta
     1501 cgacggcatc ccgcagaacg atctgcaaaa ccgaacacag gcattcctgc aactgcatgg
     1561 gttcttctga ggcaacggca ttcgacggca ttgcgcagga ctacgacgcg gagttcaccg
     1621 cgaccgccct gggttcggtg ctgcgggata tggtctggcg ccggtatgac gcgcgcttcg
     1681 cgggttgcga gagcctgctg gagatcggtt gcggcaccgg cgaagacgct atctacctgg
     1741 cgcggcgcgg gcaccgcgtg ttggccagcg acgcgtcgac acagatggtg cgcatcgccg
     1801 aagccaaggc cgaacgcgcg ggcgtggcgc accgcatccg tttcctctgc gcgcccatgg
     1861 ggtggctcgg gccggcaata gccggtgaat ccttcgacgg cttctattcg aacttcgggg
     1921 cgatcaattg cgagccgcgg ctggaagaat ccatcggcac gatcgcgccg catctggcgc
     1981 cgggcgcgcc gctcgtgtgg gtcgtcatgg ggcgttacgt gccatgggag tgggcctggt
     2041 ttatcgcgca cggtcatgca cgcaaggcgt ttcggcgcct gagccgcgac ggcgtgtcgt
     2101 ggcgcggcct gactatccac taccccgcgc cggccacgct gcggcgcata ttgcagccgt
     2161 acttcgatcc gacaggtgcc cgcgcgctgg gtttcgcgct gccgccgagc ttcgccggcg
     2221 cctggctcga tcactcgccc cgcgcactgg cggcactgac ccgcctggag cagctggcgc
     2281 agggatggag cgcatgcgct acgctcgccg atcactacat cctcgaagcg cagcgcaagc
     2341 cgtgaacgcg tacgcgccac tccgtgattc tcccgtccgg tcgtattcgg ccaagcttgc
     2401 gcggtttgcg cagttcgcgg agccggagct gcgcggcatc gtcgccggcc tgcgcctgcc
     2461 gccacgcgcc gtggtgctcg acctgggttg tggcgtcggg ctcgtgagtg cactgctcgc
     2521 cgaagcgatc ggcggcagtg gccgggtgat cggcgccgat ctgtcgctgc cgcacctgca
     2581 ggcggccaag gctaactacc ttgggccatt ggtgcaggcc gatgcggcgc gaatgtgttt
     2641 ccgcccgggt gcattcgacg tgatctggag ctgcaacacg ctcaaccatg tcgacgatcc
     2701 ggtcggcgtg ctgaagtccc tgaagccgct gctgcgcggc tccgggcgcg tcgtgctcgc
     2761 gcagagcggc tttctgccgg agatgttctt cgcctgggac gcgccgctcg acgaggcggt
     2821 gcggcgcgcg tgccacgcgt attaccgcaa tcggtattca ctcgaagtcg ccgataccgc
     2881 cgggctgcgc ggcctcgttc gcctgctgga cctggcggga taccggcgcg gcgccgtgcg
     2941 caccgtggtg atcgagcgtt tgcagccgct cggcgccgcg gacgccgcgt acttcgaaga
     3001 gacgatgttc gcgggcacct ggggcgaacg cctgaagcct ttcctcgatg cgcgcgagtg
     3061 ggacgcgctg cgccgctaca ccgatccgga atccgcggaa tactgcctgc accggcgcga
     3121 ctttcatcac ctgcagactc tcaccgtgtg tgaaggtcat ggctgagcgc gcgaccggcg
     3181 gattcaccgg cgcgctgctc gatgcggatc gcgcgcggcc gctgggcgcg gccttcgggc
     3241 gcatccttca cctgggcgcg cggctgctgc gccacgaccg ttatgacgac taccgcttcg
     3301 aacgctgcgg cgatctgcga ctcgtcgtgc tgcccacggt gtcgaatccg aagctgctgc
     3361 gcaccggcgc cttcttcgcg tcggtactga atcagcaggc cgtcgcgccc gtcacggcgc
     3421 tcgacctcgg tacgggatcg ggaatctgcg cgctggcggt ggcgccgcgt gcgaaccggg
     3481 tcgtggccgt cgacatcaat cgcgcggccg tgcgctgcgc acagatcaac gcgagcctca
     3541 acgggctcga gcacaaggtc gaggtgaggc caggcgatct gttctcgtcc gtgccgggcg
     3601 agcggttcga cctcgtgatg ttcaacccgc cgtttctgtc gggcgcgccg cgcgacgatc
     3661 gcgacgcggc gtggcggggc gccggccttg ccggaagatt cgcggcggag ctggctgatc
     3721 acctcacacc cggtggccgt gcgttgctgt tgctgtcgtc ctggggcgcc gcgtgccgcg
     3781 accacgtgga tgaactcgtg cgccgcgggt ttcgactcac cgccatcgcg cgcaaacatc
     3841 atgtgaacga aaccatcacg atcgtcgagg tcgtgccgcc atgagtccgc gcgtctccgt
     3901 catcctgccg tcgttcaacc ggctcgaatt cctgcgcacc gcggtcgatt cggtgctcgc
     3961 tcaatcgctg tccgactggg agctgatcgt catcgacgac ggctcagccg tgccggtccc
     4021 agcctatctg gattggatcg cgtcgcttgc gcgcgtgcgc gtctttcgcc gcgcgcattg
     4081 tggaaatccc gccgcggttc gcaacgccgg tattcgcgag gcccgcggcg actacgtggc
     4141 tttcctcgac tccgacgacc tgtgggccgc gcacaaactc tccgcacagc tggaggcgct
     4201 gggccgttcc ggcttgcgat ggggttacac ggcctttcgg cacatcgatg ccgccgccgc
     4261 gccgctcgtg cgctcccggt accaggcgtg gcgcccaacc gtttatcgga cgctcgaagg
     4321 cgtggtgcgg ctcgacgcca tgatcccgct gcccagcgtg atcgtggagc gcgcgttgct
     4381 cgagtccgtg ggtgggttcg acgagcgcct gcgctactac gaggactacg acctgtggtt
     4441 ccgtctcgcg ttgcagggcg aagccgatgt gctggcgacg ccgctggtga gtatccgccg
     4501 ccacgaccag cactactcgg acgtggacca ggcgttgacg cgcgagaacc tcgaccaatt
     4561 gttcgcgacg atgctcgcgc aactcgaaga cgagaacctg cgcggaatcg tgcggcgtca
     4621 gcgcgcagtc aactcggggc ggctcgcggg cgtacacgcg tcggcgcacg acgcgcgcgg
     4681 catgttgcgc gcgctgcgcc agtccttcgc gcactccaag gtgcagccgt tgtggtgggc
     4741 gggcgcggcg cgcgcgctgg ctcgctgcct gttgccgcca tgaacgcgtt ccgcgcgctg
     4801 tggacgctgc tgccgcgcga ccttcgccgg cgatttgtcg gcttgcagct gctcgcgctg
     4861 gtgatggcgt tctccacggt cgcgggactg gtctcgatca tgccgttcct cgcgttgttg
     4921 tccgaccctg gcgtgtttgc cgcaagcccg cgtttgcagg cggcccaggc tgcgctcggc
     4981 ttcagcacgc cgcgttcctt cctggtggcg atcggcatgg tgttcgtcct cacgctgttc
     5041 atcgccaatg ccgtcaacta ttttggcgca cgctccatgt atgcgttcgc gctgcgtgcc
     5101 gccgacgaaa tacgcgtgcg gctgttcggc gcctacctgc gcagcgacta tctattccac
     5161 ctgcgcaacg accacgcgcg gctcggcaac gatgtgctgc acgaatccga ccgcgtcgcg
     5221 gccgtcatcc acggcggctt ccagctgctg acggcgggcg cggtggtcac gctgatctcg
     5281 gtgtcgatcc tcgtgatgaa ccccgccatc tcgctgggcg ccatcgtggt gttcggcggg
     5341 agctacgcgc tgacttacgc gcgatcccgt cgccggcttc agcgtaatgg ccaggagctc
     5401 acgcgcgaat ccgcgcggcg cgcgacggtg gtccggcaga ctttcgcggc gatcaaggaa
     5461 ctgctggtgt cccaccgcca ggaaccgttc cagcggcagt tcgccgaggc gaccggcggc
     5521 gtgtcgcgcg ccatcgtgcg cacgcacacc atcggcatgc agccgaagta cgtattcgaa
     5581 tgcctcgccg cgctggcgct ggtggtgacg gcgcttctgc tgacttccgt ggaacagcgc
     5641 atgggttcgt ggatggcgca gctcaccttt ctcgcgctgt gtgcctatcg gctgctgccg
     5701 agcctgcagc agttgttcac gggcctggtg acggtgcgtg cgcacgcgca ggtgctgttc
     5761 acgctggcgc aacggctggc cgagcccgca accgccggcg cggcgccagc cagagacgag
     5821 gccgcgtggc gagggatgcc gcgcgccgac atcgtgttcg atcgcgtgag cttccgctac
     5881 gcggacgatt catcgctcgc cgtcgaccag gtgagcctgc gcatccgttc cggccagacc
     5941 atcggcatcg ccgggccaaa cggctccggc aagtcgacac tggccgatct cctgctcggc
     6001 ctgcttacgc ccacgagcgg gcgcatcctc gtggatggcg ccgacatctc cggtcgcaac
     6061 cgcatcgcct ggcagtcgac cgccgcctat ctaccccagc acgtggccct catcgacggg
     6121 acggtcgccg taaacattgc gctcggcgtg ccgaccgcgg acatcgacac cgcgcgactc
     6181 gaacgcgccg cccgcctggc ctgtgtcgac gaattcgtcg cgccgatgga agagggttat
     6241 cagacgcggc tcggtgaatc cggcacgcag ctgtcgggcg gacagcgtca gcgcatcggc
     6301 attgcgcgcg cgttgtatcg tgacgcctcg atgctggtgc tggacgaggc gaccagctcg
     6361 ctcgatacgg tttccgaacg cgagatcacc gcggcgctgc ggcaacggcc gccgggcgtc
     6421 acggtcgtgc tcatcgcgca tcgccgccac gcgctcgagg attgcgacgt gatcttcgag
     6481 atgcacgacg gcctcatccg cgcgagcgaa cccgccgcat gcgcgtgagc cgcctcgatc
     6541 ccttcgagga gcggccgcga cgcacctggc ggcgacgcct cacattgctc ggcgcggtcg
     6601 tggagttcga gtgcgacagc ccggcgctgc gagcggtagt cgatgcggct tatgccggaa
     6661 ttccggcgcc gcgcccccgg gcacgcgcgc cacgatactt cgtgcggatt cgcaccgcga
     6721 cggatagcgc cacctttcgc ggtgaacctc cgcagccgag actcacctcg ggagcggggc
     6781 tgctgtgcgc gagtgtcgac gcgtccaact acacggtcgt cgaccccgcg tcgcgcagcg
     6841 cgctgatcgt cgcatcgccc gcgatgctcg cgcatccgta ttacctgcgt tgcgagttcg
     6901 tggagttcgc gctgttcacg ctcgcggcgc gcgaccaggc ggtggtcgcg ctgcatgcgg
     6961 cctgcgtcgg gcacggcaat cgcggtgtgt tgctgctcgg ccaaagcggc gcgggaaaat
     7021 ccacggcgac gctgcagtcg atgctgtccg ggcttgcggt gttgtctgaa gatgtcgtgt
     7081 tcgtcgagcc gcggcgcatg cgcgcgaccg gcgtgcccaa cttcctgcat cttcggcgcg
     7141 aaagtctcag gctgctgtcg cggccgctcg cgcgccgcat tcgcagcgca ccgctgatcc
     7201 ggcgccggag cggcgtgatc aagcacgaat tcgaccttcg tcagttcgag ggcgccgcgg
     7261 cgctccagcc gttgcaactc gtggccacgg tggtgttgtc ccggcggcgc gcaaaacggg
     7321 gcgctccgtt gctcgagaaa ctcctcgtgg aaccgttgct tcgggcgctc gaccaggatc
     7381 aaccctacgc acgtacgcgc gcccactggc acgcgcttcg ccgcggcctc ggaaaaattc
     7441 ctgcctatca attgttgcgt ggcgccgatc cggcgcaggc gggcgtggag ctgcgccggc
     7501 tgctgcgcgg tactcgcgcg tgaaagtggc gctggtcgtt cccggcggcg tcgatcgtcc
     7561 cggcgggacg ctggtgattc cggcattgat gtcgctggtc ggccgtctcg ccgcgcgcca
     7621 cgagctgcgc gtcttcacgc tgtaccagga gccgcagccg tcgcgctggt cggtgctcgg
     7681 cgcccacgtc atcaatgtcg gcgcgcgaca gcgtcacgcg cgcgccgtgg ccgccatctg
     7741 ctccgaacat cggcgcgtgc ggttcgatct cgtgcatgcg atctgggcgg gctccaacgg
     7801 attggccggc gtggcggcgg cgacgctgct gcggctgccg agcctggtgc acctcgcggg
     7861 tggcgaactc gtggccatcg gcgacatcgg ttacggcggt ggcctgcgct tgcgcaatcg
     7921 cgtggccaat cgctgggccc tgcgccgcgc gtcagtcgtc acggcggcga gtgcgccgat
     7981 gctcgacgcc gcccgatctc tcggcatcga agcgatgcgc gtgccgcttg gcgtggacct
     8041 cacggcgtgg cctccgcgtg cgcctcgccg ccgtgatgtc aacgcgcccg cgagactcat
     8101 ccacgtggcc agcctcaacc tcgtcaagga ccagggttgc ctgctgcgcg cattcgcgca
     8161 gctcgtggcc gagggccgtg acgtcgagat ggacatcgtc ggcgtcgata cgctgcacgg
     8221 cagtgtgcaa gctctcgcgc aggagctcgg gctcagttcg cgcgtgcact tccatggctt
     8281 gctgggtcac gtgcagctgc gcggactttt cgagcgcgcc gacctgctgg cgatggcctc
     8341 acgccacgag gcgggtccgc tcgtgacgct cgaagcgggc gtcatcggtg ttcccacggt
     8401 ggggacgtcg gtgggccaca tcgccgaatg ggcgccgcac gcagcgctgg cttccgcggt
     8461 cggcgatccg gccgcgctcg ccgccaactt ggcgcgactg ctcgacgacg aagacctgcg
     8521 gctgcgcatc gccgccgaag cgcagcgacg tgcgctggcc gacgatgccg actgcaccgc
     8581 ccggctcttc gaaacgcagt atcaccgcgt ggtcgcgtga gcgccgccca ctcacccgtg
     8641 gcgtaccgcg cggacatcga cggactgcgc gcggtcgccg tcgtcgcggt gattctctgc
     8701 cacgccggca cgccatacct caacggcgga ttcgccggcg tcgatgtgtt cttcgtgatc
     8761 tccggctacc tgatcacgca aatactggtc cccgccactg ccggcggtga atggcggccg
     8821 atcggcgagt tctatctacg gcgcgtccgc cgcatcctgc ccgcgctctt cgtgatgttc
     8881 ggcgtggtga cgattgccgc tctggtgttg ttcgtgccgt cggagctcga gcgattcggc
     8941 gccgacctgc gcctggcgag cgtcatgctc ggcaacctcg cggggtgggg caacggcaac
     9001 tatttcgacg cgatgaccgc gcagctccca ttgctgcacg tctggtcggt ggcggtcgag
     9061 gagcagttct acctggcata cccctggctg atcctggccg tggcgcgctg gatgcccgga
     9121 cgccgcacgc tggttctgct gctctgcgcg ctcgcctcgc tggcgttctg cctgtactgc
     9181 gcgaaccggc aatacgtcgc taactactat ctcgcgccga cgcgcggctg ggaattgctg
     9241 gcgggcgcct tgctggtcgg cgtcgaatgg ccgttgttgg cgcgccgctg gttgcgcgaa
     9301 ctgctcgcgg cgctgtcgct ggtggcgctc tgtgcctgtt tcgtgttcct gcgccaggac
     9361 gattcgtggc cgaatctcac ggcgctgctg ccctgtctgg ccaccgcggg cctgctggcc
     9421 gccgggcgtg caggcccgac gctgagttcg cgaatgcttg aatggcggcc ggtcgttttc
     9481 acgggactca tttcctattc gctgtacctc tggcatgcgc cggcactcac gttcttcgaa
     9541 cgattcaaca tcgtgccgcc gagcgcctgg caactcgctg cgctgctcgc cgcggtgtac
     9601 gcggtgtcgg cgctcagttg ggcatacatc gaagaacccg ttcggcgccg ccgcgtgttg
     9661 acggactcgc ggcggctcct gtgggtggtg gcggccaccg tgctgttcct ggtggtggtg
     9721 gccacgctgc tggtgcgtac ccacggactt ccgcagcgat ttccggcgcc gctgcgcgcg
     9781 atgatccagg gagtcgacct gcatcgcgac ggcgcgcgtt gcatggggct gtcggtcgcg
     9841 caggtgaccg ccggcgagtt gtgccgcttc gggccggtcg aagatcgccg tccgcgcgtc
     9901 atcgcctggg gagacagtca cgcggccacg ctgatgcccg cgttcgaaag cgtggcgctg
     9961 tcgcgcggcg tgagcctgga tttcgcggcg cgtgccgcgt gccggccgct gctgggcgtg
    10021 accagcgagt tgcgcgccga ccgtcacgtg ccggcgtgcg agcagttcaa cgaagcgatg
    10081 acctcactcg tgaccaagct ggcgcccgac atggtgatcc tggccgcgca ctggagtgag
    10141 cccggcgacc gcttcacgtc gcccgcgctg caggcgcgat ccggcgaatc catcttccgc
    10201 gccgggctcg aacgcacggt gcagagaatc gccagcgcgc gcacgcaggt gtgcatcgtg
    10261 ctggacgtgc ccgagttgaa ctacccgata ccccacgcgt tgctcacggc ccgtcagcgc
    10321 gggttggaca ctgaattcct gcgtatcacg cgcaccgagc tcgcggcgcg ctacggctcg
    10381 accgagagcg acatccgcgc cgtggtggcg gcattccatc aggtggtcgt cgatccgaaa
    10441 gagtcgctgt gccgcggcga gcgctgcgac ttcggcaacg ccacggaggt gtactacatg
    10501 gacgccaacc acctgactac cgccggggcg agagcggtca cgcgaacgat cgacggttgc
    10561 ctggcggcgc tcgagaggcg ttagttcggg cgcgccgcga ttcgcaggcg ccgcaaggcc
    10621 ccatggaaca ggaatgacag gctctcgatg gccgtccacg cggcgatgcg cagccgcagc
    10681 gacagcgtca attcgtcgcg aacgaacagc gcgttgaatt gccggtggcg cgagtctccg
    10741 tccgacgcga tttcgcgcag cccgaaagtc gcgagcagcc gccgcacgtc cgggtacagt
    10801 ttctgcgccg cattcatgca cgggacccgc tcgacttcga cgtgcacgag ctgcaggcgc
    10861 gcggccagcg ccgaggcgcc cgcaagcgtc tcgtacgcca tgccttcggc gtctatccac
    10921 aacgcgatgc gcgcctgggg cgcggtgcgc gtggccagga atgcatcgag tcttacggtc
    10981 ggcacctcga taacctcgag cagttccgat gcgaccacgg ccggatacag cgagctgcgc
    11041 ccgcgctgcg gaccggtatt ggcgtagtcg gcccgcagca cgtgaaacgg cgaacgtgtt
    11101 tcgcgatccg cgaccgcgca cggcacgatt tcgacgcgcg cgcgctgcag atcgaggttc
    11161 gcgcgcatgg gtgcgagatt ccacggattg ggctcgaagg cgaacacgga tgcatggggt
    11221 aagcggcgcg cgaagcgcag cgcctcggtg ccgtccatcg agcccacgtc gcaaacgatt
    11281 ccggcgtcga acaatggcag cagccgttcg aacaggctcg cggtgttcac ccgcatttcg
    11341 agatttccgg acgcaccagc atttcggcgc gttcagccac cagacgcgcg tcgtagcgcg
    11401 tgagacagcg gttatcgccg aagcggcaca cctcgagccc gcagggacca cacggtagat
    11461 cggacgagtc gaggcagtcg ctccagctac cctgcggcgc ggaccagtcc ttgcggctgg
    11521 tgctgaacag cgccagcgtc ggcgtgccct gcacccagga catgtgcatg agacccgagt
    11581 cttccgacaa cacgagacgt gcgcgtccgg cgatcgcgaa tgcctgcagc tgatcggcgt
    11641 gacccgtgag atcgacacag gcgtcgccga gcgcggcggc aatggcgcgg gacttgtcgc
    11701 gctgcgaagg cagcagcaac agcacgatct gggttcgcgg atgtcgcgcg atccacagtc
    11761 gcgcgaattc gcaatagttc tcgatcggcc acgcgcgcgc cggtgaatat ccggccggat
    11821 tcagcaccac gagttcgtgg cccggtttcc agccgtgctc gcgcagcagc gtgtcgacat
    11881 ccgggtcgcg acgcagcgtg agaccggtgt cgagggccgg tcggcaatcg atggcggctg
    11941 cgatggtgcg gcggttgcgt tcgccggcgg agcgcggcga aaagcggtcg aactcggccc
    12001 aggcgcgcgg cgcgatcagc cggcgtacca gccggctgat gtggctgttc tgcaggtcga
    12061 gcaccaggtc gtaacgctgc gccagcaacg acggcatgcg gcccatgacg gacagcagct
    12121 gccacattcg gctgcgtccg ccgccgaatg tgaacacgcg gtcgaacaca tcgaccgcgg
    12181 cgggtacctg gcacacctcg tcgcgcgtga ggaaatgcaa atcggcctgc gggaatcgcc
    12241 gcttcaggct gagtagatag ggcaacgtga tgagcgtgtc gccgagcgcc tggaagcgca
    12301 tggcgaggat gagccgcggc gactcctgcg agcgccacgg cttgcaggag accggcggct
    12361 cgccgtcgcg cggcgtcatg ggcgggcctg tccggcgagc accgtcaaca gggtctgcac
    12421 tcgcggcggc cgcgaggtca gccagcgcag gatacgcatg ccgtgaggct gcgcgtacca
    12481 cgcgatgcgc agcaggtctg gctggttctt caccgtgtcc gccagttcgc tgaacgcctg
    12541 gcgaggcggc agcaggcggc tgcgcacgaa gcgcgccagc tccatcgggc ttcgcgacca
    12601 ttcaaatcct ggcagcgcgg cgatccgcag gttggaccac gacacgtcgt tcaaggcctg
    12661 gcgctgcgac ttcacgcgaa gcagcgtggg gctgatcgcg cgagcgccgg cgagtacttc
    12721 gtcgggcacc gtgtgaggca tgaggcgcgc cgtcagcgcc agcaccggga acatccacca
    12781 cgcgcgcgca ggttgcgcgg gcgatcccag caactgcgcc cactgcgccg catccatgcg
    12841 ccgcgccagc aaggcgatgt cgacgagctg gatgtagcgc agcgcattcg tgcgcatgtt
    12901 tcccgcggca tgcagcagca ggtgacacat gagcgcggcg gtgtcgcgat aggcattggc
    12961 gccggagcgc gtcccggccg gccagatgcg ctcggtgacg tcgatgaggc ttacgggcag
    13021 attctcggtg acggtcgtat gcagctcgat acggaacggc acatccgggt gctcgccgaa
    13081 gcggcgcggg accggacgat ccgcaggcac gaacactgtg tgccgctgac aagaatgcgt
    13141 cggcgtgtag ccgatggagc ggagcgtggc gctcaagcgg tctgcctgcc gcggcgaaac
    13201 cagcagatcc acgtcgctca
//
LOCUS       DATDEU010000664         5684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_274654, whole genome shotgun sequence.
ACCESSION   DATDEU010000664 DATDEU010000000
VERSION     DATDEU010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274654"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="VIV63_15900"
     CDS             <1..1454
                     /locus_tag="VIV63_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126136.1"
                     /translation="GATRYELWRRASESGTWLKVQDRPAAATYTIIAVSAHLLDWRGA
                     RWRVDACDASGCTPSAEVSATSLMRDSVGFIKPVPELPPRRFGMAMALSADGRTLAVL
                     GAEFWTNISLLPYVYVYRRVGSNWVFDARLASDNARENDNVGTSVALNSDGSVLVWGT
                     QFTPYPNDTGGTVFRGAVYVFRRVAGAWQRDHKVTAPNEQTMYLGRDVDVSDDGERFV
                     ASYQSLPYRMGHTQIYHRVSGAWQLEKTLTMRTSGSHWAECIGLALSGNGQRVARYCT
                     RSPHSPALAGKFVETYSAPRWTLEAEIQLGSHIHYPDFLRVIGAIDISGDGTRLVSMH
                     SHESTLSVWRWNGSAWEADSPAGALAELTTPGLSVAMSRNGNLIVQGDEHDVTVGVGA
                     IYPPYQVGVASTGIARVYERRGGTWTLRQMIKGNSPASAAYYRQGFGSTVALGDNGSV
                     LAVGAESNSSAATGVDGDQDDISAPYSGAAWLY"
     gene            complement(1464..3674)
                     /gene="paoC"
                     /locus_tag="VIV63_15905"
     CDS             complement(1464..3674)
                     /gene="paoC"
                     /locus_tag="VIV63_15905"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF041671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HEY6126137.1"
                     /translation="MKHDKPATQNPTDRKKVIGKPTDRYEGKLKTTGTAKYAYEWHDI
                     APKPAYGYVLGAAIAKGRIAAIDTAAAESAPGVYAVVTAENAGPLEKGNFNTAKLLAG
                     PEVQHYHQAVALVVARTFEEARAAAARIKVRYATEPGAYDLSKAKDSAQKPKAQFGSE
                     PDSGAGDFNGAFAAAPVKLDATYTTPDHTHAMMEPFATIAAWKGDELTVWTANQMIAW
                     ARGDLAKTLKMDPAKVRVVSPYIGGGFGGKLFLRSDVLLAALGARAAKRPVKVVLPRP
                     LMANNSTHRPATIQRVRLGATPDGKITAFAHETWSGNLPGGSAEMGILASRALYAGAN
                     RMTATRLATLDLPEGNAMRAPGDAPGSMAFEIAIDEMAHQLKLDPVEFRILNDTQKDP
                     EKPSRVYSTRKLVECLRTGADKFGWSRRNAQPGKVRDGRWLVGMGVATAYRGAPVLDS
                     AARVLLNAQGIVTVETDMTDIGTGSYTIIQQTAAETMGVPLDRVAVNLGDSRFPVSAG
                     SGGQFGAVSATSGVYAACMKLREAIAQKLGVDPATADFTDGVVVAGGKRTPLGEAAGP
                     QGLSAEDKIEFALDREKYLYYTFGAHFVEVAVDSLTGETRVRRMLAVCSAGRILNPKA
                     ARSQIIGAMTMGVGAALMEDLVVDKRLGFFVNHDLAGYEVPVHADIPHQDVIFLDDED
                     PTLSPMKAMGVGELGICGVPAAVANAIYNATGIRVREYPITLDKLLPKLGVRSA"
     gene            complement(3671..4627)
                     /locus_tag="VIV63_15910"
     CDS             complement(3671..4627)
                     /locus_tag="VIV63_15910"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY6126138.1"
                     /translation="MKPFSYENAATPAAAAAAAARNPGAKFIAGGTNLLDLMKLEIES
                     PSHLIDVNDLALNKIESTPDGGMRIGALVRNTDLAADTRVRREYPLLSRALLAGASGQ
                     LRNSATTAGNLLQRTRCPYFYDTNQACNKREPGKGCAALQGFSRQLAVVGSSNACIAT
                     HPSDMAIAMRAFDATVETVQPDGTARKIPIADFHTLPGDTPHIENTLKRGELITAVTL
                     PKPVGGKQLYHKVRDRSSYAFALVSVGLIVQPDGSGRVAVGGVAHKPWRVEAAEAELP
                     RGAKAVTTRLLADAKPTHDNEFKITLVERTLEAVLADARSAG"
     gene            complement(4624..5241)
                     /gene="paoA"
                     /locus_tag="VIV63_15915"
     CDS             complement(4624..5241)
                     /gene="paoA"
                     /locus_tag="VIV63_15915"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008514.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HEY6126139.1"
                     /translation="MQGPGNFDLSRRDVLKLGAAATAAPMVANAQQAPADAAVVSTVT
                     LNVNGKTHTLKLDTRTTLLDALRENLQLTGSKKGCDHGQCGACTVLIDGRRTNSCLTL
                     AVMHQRHKVTTIEGLGTPTSMHPMQTAFVKHDGFQCGYCTPGQICSAVGVLAEIKQGI
                     PSHATADLNATAQATPAEIRERMSGNLCRCGAYSNIVDAITEVAT"
     gene            5447..>5684
                     /locus_tag="VIV63_15920"
     CDS             5447..>5684
                     /locus_tag="VIV63_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126140.1"
                     /translation="MHLRISVLLPAWIVLCTLLVPAVSSAEPLQILEQGSFLVGGKTI
                     ATPGKFDPYKPTPEGQSFSGDHAYVFYQVPVKPRK"
BASE COUNT          909 a         1807 c         1877 g         1091 t
ORIGIN      
        1 cgggagcaac ccgatacgag ttgtggcggc gcgcttccga gagcggcacg tggctcaagg
       61 tgcaggatcg gcctgcggcc gcgacctaca cgatcatcgc agtgtccgcg cacctgctcg
      121 attggcgcgg agcgcgatgg cgtgtcgatg cctgcgatgc gagtggttgc acgccgtcgg
      181 cggaagtttc cgcgacgagc ctcatgcgcg actcggtcgg cttcatcaaa cctgtgcccg
      241 agctgccccc aaggcgtttc ggaatggcga tggcactcag tgccgacggt cgaacactgg
      301 cggtgttagg cgcggagttc tggacgaaca tctctctact accgtacgtc tacgtctatc
      361 ggcgtgtggg gtcgaactgg gttttcgatg cccgcttggc gtccgacaac gcgcgggaga
      421 atgacaacgt tggaacttcg gtggcgctca actccgatgg cagcgtgctg gtatggggca
      481 cgcagttcac cccttatccg aatgacacgg gcggcacggt ctttcgtggc gcggtttatg
      541 tgtttcggcg cgtagccggc gcgtggcaac gagaccacaa agtgacggcg cccaatgagc
      601 agacgatgta cctgggccgc gacgtcgatg tgtccgacga tggtgagcgc ttcgtggcga
      661 gctaccagag tctgccctat cgcatggggc acactcagat ttaccaccgg gtctcgggtg
      721 cctggcagct cgagaaaacg ctgacgatgc gcacgagtgg atcgcactgg gccgaatgta
      781 ttgggctcgc gttgtcaggc aacggccagc gtgtcgcgag gtactgcaca cgcagtcctc
      841 acagtccggc gctcgccggc aaattcgtcg agacatactc cgcgccccgg tggacattag
      901 aggccgaaat tcagctcggt tcgcacatcc actaccccga tttcctgcgc gtgattggcg
      961 cgatcgatat cagcggcgac ggcacgcggc tcgtctcgat gcactcgcac gaaagcacac
     1021 tctcggtctg gcgatggaat gggtcggcgt gggaagccga cagtccggcg ggcgcgttgg
     1081 cggaactcac cacgcccggt ctcagtgtgg cgatgagtcg caacggaaac ctgatcgtgc
     1141 agggcgatga gcatgacgtc accgtgggcg tcggtgcaat ctatccaccc tatcaagttg
     1201 gcgtcgccag caccggcatt gcgcgcgtgt acgaacgccg cggcggcacg tggacgttac
     1261 ggcagatgat caagggcaac tcgcccgcgt cggcggcgta ctatcgccag ggcttcggtt
     1321 cgacggtcgc gctgggcgac aacggcagcg tgctcgcggt gggcgcggag tccaacagca
     1381 gcgcggcgac cggcgtcgac ggtgatcagg acgacatttc ggctccctac agcggagccg
     1441 cgtggttgta ctgagtgcaa ccctcaggcc gagcgcacac ccagcttcgg caatagcttg
     1501 tcgagcgtga tcggatactc gcgcacgcgg attcccgtgg cgttgtagat cgcattcgcg
     1561 accgcggccg ggactccgca aatgcccagc tccccgacgc ccatcgcttt catcggcgac
     1621 agcgtcgggt cttcatcgtc gaggaagatg acgtcctgat gcgggatgtc ggcgtgcacg
     1681 ggcacttcgt agccggcgag gtcgtgattg acgaagaacc cgaggcgctt gtccaccacg
     1741 agatcttcca tgagcgcggc gcctacaccc atggtcatgg cgccgatgat ctggctgcgc
     1801 gccgccttcg ggttgaggat tctgcctgcc gaacacaccg cgagcatgcg tcgcacgcgc
     1861 gtttcccccg tcagcgagtc caccgcgact tcgacgaagt gcgcgccgaa cgtgtagtaa
     1921 aggtacttct cccgatccag cgcgaactcg atcttgtcct cggcggacag gccctgcgga
     1981 cctgcagcct cgcccagcgg cgtgcgtttg ccgcccgcga cgaccacgcc atccgtgaag
     2041 tcggcggtcg ccgggtccac gcctaacttc tgcgcaatgg cctcacgcag cttcatgcac
     2101 gcggcgtaaa cgccggaggt ggcgctcacg gcgccgaact gtccaccgga accggcagag
     2161 accggaaagc gggagtcgcc gagattcacg gcgacgcgat cgagcggcac acccatcgtc
     2221 tcggcggcgg tctgctggat gatggtgtag ctgccggtgc cgatgtccgt catgtccgtt
     2281 tcgacggtca cgataccttg cgcattgagc agcacacgcg ccgcggagtc gagcactggc
     2341 gcgccgcgat aggccgtggc gacacccatt cccacgagcc agcggccatc acgcaccttg
     2401 ccgggctgcg cattgcgacg gctccagccg aatttatccg cgccggtgcg caggcattcg
     2461 acgagcttgc gagtggagta cacgcgcgac ggtttctcgg gatctttctg cgtgtcgttg
     2521 aggatgcgga attcgacggg atccagcttc aactggtgcg ccatctcgtc gatggcaatt
     2581 tcgaacgcca tcgagccagg cgcatcgccg ggcgcgcgca tcgcattgcc ttcgggcagg
     2641 tcgagagtcg caaggcgcgt ggcggtcatc cggttcgcgc cggcatacaa ggcgcgcgac
     2701 gccaggatgc ccatctccgc actgccgccc ggcaggttgc cggaccaggt ttcatgcgcg
     2761 aaggccgtga tctttccatc gggcgtcgcg ccgagacgaa cgcgctggat cgtcgcgggg
     2821 cggtgcgtgg agttgttagc catcagcggg cggggcagca ccactttcac gggccgcttc
     2881 gcggcgcgcg cgccgagcgc tgccagcagc acatccgacc gaaggaatag ttttccgccg
     2941 aatccgccgc cgatgtacgg cgacaccacg cgcactttcg ccggatccat cttgagcgtc
     3001 ttggcgaggt cgccgcgcgc ccacgcgatc atctgattcg ccgtccacac cgtgagctca
     3061 tcgcctttcc aggccgcgat cgtggcgaac ggctccatca tcgcgtgcgt gtggtcaggc
     3121 gtggtgtacg tggcgtccag cttcacgggc gccgccgcga acgcgccgtt gaaatcgccg
     3181 gccccggagt cgggctcgct gccgaattgc gctttgggct tctgcgccga gtccttcgcc
     3241 ttcgacaggt cgtacgcacc gggttcagtc gcgtagcgca ccttgatcct ggctgccgcc
     3301 gcgcgcgctt cttcgaacgt gcgcgcgacc accagggcga cggcctggtg gtagtgctga
     3361 acttccggtc ccgcgagcaa tttcgcggtg ttgaagtttc ctttctcgag cggcccggca
     3421 ttttcggcag tcacgacggc atacacgccg ggcgcggatt ccgccgcggc cgtgtcgatg
     3481 gcggcgatgc ggcccttggc gatcgcggcg ccgagcacat agccgtacgc cggcttggga
     3541 gcgatgtcgt gccactcgta ggcgtatttc gccgtgcccg tggtcttgag tttgccctcg
     3601 tagcgatccg tgggtttgcc gatgaccttc ttgcggtccg tgggattctg ggttgccggc
     3661 ttgtcgtgtt tcatcccgcg ctcctcgcgt cagccagcac cgcttcgagc gtgcgctcga
     3721 ccagcgtgat cttgaattcg ttgtcgtgcg tgggcttcgc atccgccagc aatcgggtgg
     3781 tcacggcttt cgccccgcgc ggcaactcgg cttccgcggc ctcgacgcgc cagggtttgt
     3841 gtgcgacgcc gcccaccgcc acgcggccgc tgccatcggg ctgaacgatc aaccccacgg
     3901 atacgagcgc gaaggcgtac gacgagcggt cgcgcacctt gtgatagagc tgtttgccgc
     3961 cgacgggttt gggcaacgtc actgccgtga tcagctcgcc acgcttcaat gtgttctcga
     4021 tgtgcggcgt gtcaccgggc agcgtgtgaa agtccgcgat gggaatcttc cgcgccgtgc
     4081 cgtcgggctg caccgtctcg acggtcgcat cgaacgcgcg catcgcgatc gccatgtccg
     4141 aagggtgcgt ggcgatacag gcattgctcg agccgacgac ggcgagctgg cgactgaaac
     4201 cctgcagcgc tgcacaacct ttgccgggtt cgcgcttgtt gcaggcctgg ttcgtatcgt
     4261 agaagtacgg gcagcgagtg cgttgcagca ggtttcccgc cgtggtcgcg ctgttgcgta
     4321 actggccgga cgcgcccgcc agcaacgcgc gcgacaacaa cggatattcg cgccgcaccc
     4381 gcgtgtcggc ggcgaggtcc gtattgcgca ccagcgcgcc gatgcgcatg ccgccgtcgg
     4441 gcgtgctctc gatcttgttc agcgcgaggt cattgacgtc gatgagatgc gacggacttt
     4501 cgatctcgag cttcatgaga tccagcaggt tggtgccgcc cgcgatgaac ttggcgcccg
     4561 ggttgcgcgc cgccgccgca gcggccgcgg cgggcgtggc cgcgttttcg tagctgaagg
     4621 gtttcatgtg gccacctcgg tgatggcgtc gacgatgttg gagtacgcgc cgcaccggca
     4681 gaggttgccg ctcatgcgct cgcggatttc ggcgggcgtc gcttgcgcgg tggcattgag
     4741 atcggcggtt gcgtgactcg gaatgccttg cttgatttcc gcgagtacgc ccaccgcgga
     4801 gcagatctga cccggagtgc agtacccgca ctggaagccg tcatgtttga cgaaggccgt
     4861 ctgcatcgga tgcatgctgg tgggtgttcc caggccctcg atggtcgtca ccttgtgccg
     4921 ctgatgcatg accgccagcg tcaggcacga attggtgcgc cgcccgtcga tgagaaccgt
     4981 acaagcgccg cattgcccat ggtcgcagcc tttcttcgag cccgtgagct ggaggttctc
     5041 gcgcagcgca tccagcagcg tcgttcgcgt gtcgagtttg agtgtgtggg tcttgccgtt
     5101 gacgttgagc gtcacggtgg acacgaccgc ggcgtcggcg ggcgcctgct gcgcgttggc
     5161 caccatcggc gcggccgtgg cggcggcgcc aagcttcaga acgtcgcgcc tggaaagatc
     5221 gaaattgccg ggaccttgca tgggatctcc cgagtgggtt tccgaacggg ctgaacatac
     5281 ccgccttttg agaattggct gtcagcgaag tccccgattt ggggtgacca ggcttggcga
     5341 ttctggcaga accattcatg actggccttc ataggtcctt gcaccgcaaa cgccttattc
     5401 gagagcgcag gttctcctaa catgaggcgc aaacctggag cgattcatgc accttcgcat
     5461 ttccgttctc ctgcccgcat ggattgtgct gtgcaccctg ctggtccccg ccgtgtcgtc
     5521 cgccgaaccc ctgcagatcc tggagcaggg cagtttcctc gtcggcggca agacgattgc
     5581 gaccccgggc aagtttgacc catacaagcc aacacccgag gggcaatctt tcagcggtga
     5641 ccacgcgtac gtgttttacc aggtgccggt gaagccccgg aaat
//
LOCUS       DATDEU010000665         3666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2171586, whole genome shotgun sequence.
ACCESSION   DATDEU010000665 DATDEU010000000
VERSION     DATDEU010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171586"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1191
                     /locus_tag="VIV63_15925"
     CDS             274..1191
                     /locus_tag="VIV63_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6126141.1"
                     /translation="MDRRSALGFGTATAAVMSLAGSSSRAEGAARKAARVGPYIERPD
                     GTSLFFKDWGTGDPVVFVHSAGNSSDVWAYSMIHIVKQGFRCVAFDRRGHGRSSVPGG
                     GYDYNTLADDLAGVIEAFDLRKVTLVGHSMGCGEVVRYLTLHGSKRVARIALVAPTLP
                     FLLRTPDNPNGIPKEYFDRMRAEWTRDYPKWLAANSAPFFTPETSKEMIQWGIGLAYQ
                     TSLHAAIESNVAVTETDFREELKKIDVPTLIVQGNADKSCPIEMTGKPTAGLIHGSEL
                     RVYQGAPHGVLLTHVEQLNADLMNFLRAN"
     gene            complement(1230..2327)
                     /locus_tag="VIV63_15930"
     CDS             complement(1230..2327)
                     /locus_tag="VIV63_15930"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6126142.1"
                     /translation="MQIRRRDALKILGGATLATLADSAFAKAKPLKVLILGGTGFIGP
                     HFIDALTAGGHKITLFNRGKRDPEVREGIEQLLGDRDGQVDSLKGRDWDVVIDNSGYK
                     PSQVRLTTELLEEHCKQYIFISSIAVYHNFAKAGVDEDYELATLKDPTNETVTGETYG
                     GLKALCEKIVEAVYKERGTIIRPTYIAGPGDHTDRFTYWPWRVAKGGEMLAPGTPADP
                     FQYIDVRDLADFIRTCAEKNVTGKYNLCGPQGAVTMGSLLEEAKRITKANTTFVWASP
                     EFLTAQEIIGEKAKGNFMPIWQPPVGDQAGLLLVSPARARKKGLKFRSLETTIRDTLE
                     WQKTRPADKQVLKAGLTMEKETELLGKLKGG"
     gene            complement(2438..2653)
                     /locus_tag="VIV63_15935"
     CDS             complement(2438..2653)
                     /locus_tag="VIV63_15935"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY6126143.1"
                     /translation="MSIERVVVAFSGFMILLSLVLAFFVSPYWLAFAAIIALNLSQSA
                     FTGFCPIAYVLKSAGMGPDAELEKQQS"
     gene            complement(2750..>3666)
                     /locus_tag="VIV63_15940"
     CDS             complement(2750..>3666)
                     /locus_tag="VIV63_15940"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEY6126144.1"
                     /translation="SAHPLLERDGSAWNFGSLDFFGTTGLLIWRIGADGALVRTAVLE
                     SSELGYLHSFAMTQKYLVFTFMPYRMAEDKGSFFERLQFTQDRPCRVALVPKDALDSP
                     RWFEAPFAAIYHFADAYEHRGEVVVRAARHADIEEARAPMATAMRGERGAPSSNTELV
                     SLRLDLARNRARWETHGVGGFEFPTFDARTPAGQPALLYAPASVKPSNAPFFNSIASI
                     DTRADRVNVHHYGADILAEEHLFVPRPGSSKPGDGWLVGTLLDFKKGRSGVTVLDAAR
                     VKDGPIATAWVPYTVPLGFHGWFDARVL"
BASE COUNT          675 a         1153 c         1159 g          679 t
ORIGIN      
        1 cgagccgctc cgccagtcgc aataccatct cgcggctcgg ttgcgagcgg ccggtctcga
       61 tgaaactcaa atggcgtgtc gacatctcga catcgccggc gagatcgagc tggctcaggt
      121 gccggcgctg gcgccagtcg cgcagcgttt tgccgaaagt gtgtttttgc atggtgaaag
      181 tgtaaccggc tgtcgccgcg caaccattac ctgccaggta atcgacggcg tgcgccggcg
      241 gcgaaatact cggcacccac aaaggaggac atgatggatc gacgcagcgc actcgggttc
      301 gggaccgcca cagcggcagt gatgagcctt gcggggagca gttcgcgagc cgaaggcgcc
      361 gcacgcaagg ccgcccgcgt cgggccgtac atcgagcgcc cggacggcac ctcgctcttc
      421 ttcaaggatt ggggcaccgg cgatcctgtc gtgttcgtcc acagcgccgg caacagttca
      481 gacgtgtggg cgtattccat gatccacatc gtcaagcagg ggttccgctg cgtcgcgttc
      541 gatcggcgcg ggcatgggcg atccagcgtc cccggaggcg gctatgacta caacacgctc
      601 gccgatgatc tcgccggtgt catcgaggcg ttcgatctgc gcaaggtcac gctcgtgggg
      661 cattcgatgg gctgcggaga ggtcgttcgg tatctcacgc tccatggatc gaagcgcgtc
      721 gcgcgcatcg ctctcgtggc gcccactctg cccttcctgc tgcgcacgcc tgacaacccg
      781 aacggcatac cgaaggagta cttcgatcgc atgcgtgccg agtggacgcg cgactatccg
      841 aagtggttag cggctaactc cgcgccgttc ttcacgccgg agacgtcgaa ggagatgatt
      901 cagtggggta tcgggttggc gtaccagacg tcgttgcatg cggcgatcga aagcaacgta
      961 gccgtgaccg agacagactt cagggaggag ctcaagaaga ttgatgtgcc cacgctgatc
     1021 gtgcagggga atgcagacaa gtcatgcccg atcgagatga cggggaaacc gacggcgggg
     1081 ctgatccatg gcagcgagct ccgggtttac caaggcgcac cccacggagt ccttttgacg
     1141 catgtagagc agttgaacgc ggatctgatg aactttctcc gagccaactg atatggcgtg
     1201 cgcttgcgaa cgcgcccaga ccgcctcgct tacccgcctt tcagtttccc aagaagttct
     1261 gtttccttct ccatcgtcaa cccggccttc aacacctgct tgtccgcggg ccgcgtcttc
     1321 tgccactcca gcgtgtcgcg gatcgtggtc tccaggctcc ggaacttgag ccccttcttc
     1381 ctcgcccgcg ccgggcttac taacaacagc cccgcctgat cccccaccgg cggctgccag
     1441 atcggcatga agttgccctt ggccttctcg ccgatgatct cctgagccgt caggaattcc
     1501 gggctcgccc agacaaacgt ggtgttggcc ttggtgatgc gcttggcttc ctcgagcagc
     1561 gatcccatgg tcacggcgcc ctgcgggcca cagaggttgt acttgccggt cacattcttc
     1621 tcggcgcagg tgcgaatgaa atccgcgagg tcgcgcacgt cgatgtactg gaacggatcg
     1681 gcgggcgtgc cgggcgcgag catctcgccg cccttggcca cgcgccacgg ccagtacgta
     1741 aagcggtcgg tgtgatcgcc aggcccggca atgtaggtag gccggatgat ggtgccgcgt
     1801 tccttgtaca cggcctcgac gatcttctcg catagcgctt tgagcccgcc gtaggtttcg
     1861 cccgtgacag tctcgttggt cgggtccttg agcgtcgcca gctcataatc ttcgtcgacg
     1921 ccggccttgg cgaagttgtg atagacggcg atgctcgaaa tgaagatgta ctgcttgcag
     1981 tgctcttcga gcagttcggt ggtgagccgc acctggctcg gcttgtagcc ggagttgtcg
     2041 atgacgacgt cccagtcacg gcccttgagc gaatcgacct ggccgtcgcg atcgcctaac
     2101 agctgctcga tgccctcgcg cacttccgga tcgcgcttgc cgcgattgaa caacgtgatc
     2161 ttgtggccgc cggcggtcag cgcatcgatg aagtgcgggc cgatgaaccc ggtgccgccg
     2221 agaatgagca ccttgagcgg tttcgccttg gcgaaggcgg aatcagcaag cgtggcgagt
     2281 gtcgcgccac cgagaatctt gagtgcatcg cgtcggcgga tttgcatggg ctatcaccgt
     2341 gttggacgat gccccggaga atacccgacg caacctcgat tgctacacgg cctggcgcgg
     2401 cacacccgcg cgctggcgcc aggaagtggc gagtggacta gctctgctgc ttctccaact
     2461 ccgcatccgg ccccatgccc gccgacttca gcacatacgc gatggggcaa aacccggtga
     2521 acgcggactg ggagaggttg agggcgatga tggctgcgaa tgccagccaa tagggactca
     2581 cgaaaaaggc gagcaccagg ctcagcagga tcatgaaccc cgagaaggcg acgaccacgc
     2641 gttcgatgga catgtttgct ccctgtgcag tcggagcatt cacgcacgaa tgggggcgcg
     2701 cggagaatca gggctggtac gtatcaggct gccatcatga cgtttcgcgt caaagcacgc
     2761 gcgcgtcgaa ccatccatga aaccccagcg gcacggtgta cggcacccac gcggtcgcga
     2821 tcggcccgtc ttttactcgc gctgcatcaa gaaccgtcac accgcttcgc ccttttttga
     2881 agtcgagcag cgtgccgacc agccagccat caccgggctt gctcgaacct ggccgcggca
     2941 cgaacagatg ttcctccgcg aggatgtcgg ctccgtagtg atgcacgttc acgcgatcgg
     3001 cgcgcgtgtc gatgctcgcg atcgaattga agaacggcgc attcgatggt ttgacggagg
     3061 ccggcgcata cagcagcgcg ggctggccgg caggcgttcg cgcatcgaac gtcgggaact
     3121 cgaacccgcc cacgccgtgc gtttcccatc gcgcacggtt gcgagccagg tcgagccgca
     3181 gactcacgag ttcggtattc gaactcggcg ctccacgctc accgcgcatg gcggtggcca
     3241 tcggcgccct cgcctcttcg atgtccgcat gccgggccgc gcgcaccacc acttcgccgc
     3301 gatgctcata ggcatcggcg aagtgataga tagccgcgaa cggcgcttcg aaccagcgcg
     3361 gagagtcgag cgcatccttg ggaaccagcg caacgcggca ggggcgatcc tgcgtgaact
     3421 gcaggcgctc gaaaaacgag cccttgtcct cggccatgcg atacggcatg aacgtgaaca
     3481 ccaggtattt ctgcgtcatc gcaaagctgt gcaggtatcc gagctcgctg gactccagca
     3541 cggcagtgcg caccagcgca ccgtcggcgc cgatgcgcca gatgagcagg ccggtggtgc
     3601 cgaagaaatc gagcgatccg aagttccacg cgctgccgtc acgttcgagc agcggatgtg
     3661 cagaga
//
LOCUS       DATDEU010000666         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1005391, whole genome shotgun sequence.
ACCESSION   DATDEU010000666 DATDEU010000000
VERSION     DATDEU010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005391"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /gene="manD"
                     /locus_tag="VIV63_15945"
     CDS             <1..1066
                     /gene="manD"
                     /locus_tag="VIV63_15945"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF011654.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HEY6126145.1"
                     /translation="DHLAPNLIGRDAGRIEDIWQFFYRGAYWRRGPVTMTAIAAVDVA
                     LWDILGKMSGQPLYQLLGGRSREGALVYGHANGKDIDEASEEVGRYIAQGYRAVRAQC
                     GVPGIKKAYGISNLKNAYEPAESELPAETVWATPKYLAIVPKLFERLRKDHGPDIELL
                     HDVHHRLSPIEAARLARDLEPYRLFWLEDSTPAETQKSFELVRKHTGPPLAVGVVCNS
                     IWDCKHLIENQLIDYIRTTIVHGGGITHVRRIADFAALHGVRTGFHGATDLSPACMGA
                     ALHFDTWVPNFGIQEYMKHAPETDEVFPHDYTFKDGRLFCGETPGHGVTIDEKLAAKY
                     PYSPKQLPIARLEDGTMWDW"
     gene            complement(1088..1876)
                     /locus_tag="VIV63_15950"
     CDS             complement(1088..1876)
                     /locus_tag="VIV63_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126146.1"
                     /translation="MSTRILLVILLVIPVMASAKDKVVPLPESAVAALSGKSFVVTRH
                     DKAGFTAMTAGKAAFGLFGAGAMISAGNKIVADNAIGDPADILERELAPAVAKQYGLQ
                     LKAGPSPVIKASKPKEIAATQPDVDYVLDVESTGWMFAYYATDWDKYWVGYSAHVKFI
                     ERASGKLIADAFCNATTNKHAASPSKDSLLENNAQLLKDVTTAQGWACVHVLAKEQFR
                     LPDGVATPTPAEYADPLTAYAQAKGSAVPATPAAAEAAAVPAAN"
     gene            complement(1915..>2107)
                     /locus_tag="VIV63_15955"
     CDS             complement(1915..>2107)
                     /locus_tag="VIV63_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126147.1"
                     /translation="VAASPVYRLLGKRALDTTEFPPIETALTAGELAYRQHAGGHTSY
                     PNWPTFLDFAGRYFSRAGG"
BASE COUNT          385 a          673 c          657 g          392 t
ORIGIN      
        1 ggaccacctg gcgccgaatc tgatcggccg cgacgccggg cgcatcgaag acatctggca
       61 gtttttttat cgcggtgcgt actggcgccg cggtccggtg accatgaccg ccatcgccgc
      121 ggtcgacgtc gcgctgtggg acatcctcgg caagatgagc ggccagccgc tgtaccagct
      181 gctcggcggg cgctcgcgcg aaggcgctct cgtatatggg cacgcgaacg gcaaggacat
      241 cgacgaggcg agcgaagaag tcgggagata catagcccag ggctacaggg cggttcgcgc
      301 gcaatgcggc gtgcccggca tcaagaaggc ctacggcatt tccaatctca agaatgccta
      361 cgagccggcc gagagcgaac tgcccgcgga gactgtgtgg gcgacgccga aatacctggc
      421 gatcgttccg aaactcttcg agcgcctgcg caaggatcat ggccccgaca tcgagttgct
      481 gcatgacgtg catcatcgcc tctcgcccat cgaagccgcg cgactggccc gcgacctcga
      541 gccctatcga ctgttctggc tggaagactc cactcccgcg gagactcaga agagcttcga
      601 gctcgtgcgc aaacacacgg ggccgccgct cgcggtcggt gtagtgtgca actcgatctg
      661 ggactgcaaa catctcatcg agaatcagct catcgattac atccgcacca ccatcgtgca
      721 cggcggcggc atcacacatg ttcgtcgcat tgcggacttc gcggcacttc atggcgtgcg
      781 cacgggattc cacggcgcca ccgacctgtc accggcgtgc atgggcgccg ctctgcactt
      841 cgacacctgg gtaccgaact tcggcatcca ggaatacatg aagcatgcac ccgagaccga
      901 cgaggtgttt ccgcacgact acactttcaa ggatggtcgc ctgttctgcg gcgagacccc
      961 gggccatggg gtgaccatcg acgaaaagct tgcggcgaag tatccctact cgcccaagca
     1021 gctccccatc gcccgtctcg aagacggcac gatgtgggac tggtaggggt tcgcccccat
     1081 tccctgacta gttagccgcc ggtacggccg ctgcttcggc ggccgccggt gtcgctggca
     1141 cagcggatcc tttcgcctgc gcgtaagcgg tcagaggatc ggcatattcc gcgggtgtcg
     1201 gcgttgcgac gccgtccggc aaccggaatt gctctttcgc gagtacgtgc acacacgccc
     1261 agccctgggc ggtggtcacg tccttgagca attgcgcgtt gttctccagc agcgagtcct
     1321 tgctcggtga tgcggcatgt ttgttggtgg tcgcattgca gaatgcatcg gcgatcaact
     1381 tgccggacgc gcgctcgatg aacttcacgt gcgcgctgta gccgacccag tacttgtccc
     1441 agtcggtcgc gtagtaggca aacatccagc ccgtggattc cacatcgagc acgtagtcga
     1501 cgtcaggctg ggtggccgcg atttccttgg gcttgctggc cttgatgacc ggcgacggtc
     1561 ctgccttcaa ctgcagtccg tattgtttcg caacggccgg cgcgagctcg cgctcgagga
     1621 tgtccgcggg atcgccgatg gcgttgtcgg caacgatctt gttgccggcg gagatcatgg
     1681 cgccggcgcc aaacaacccg aatgcggcct tgcccgcggt catggccgtg aaacccgcct
     1741 tgtcatggcg ggtcaccacg aatgatttgc cactgagcgc agccacggcg ctttcaggca
     1801 gcgggaccac cttgtccttg gccgaagcca tgaccgggat caccagcagt atgactaaca
     1861 agatgcgcgt cgacatggtc ccctcccagg aatccttgtg cgtccggatc ttatctaacc
     1921 acccgccctg ctgaaatagc gcccggcaaa atcgaggaag gtcggccagt tcgggtacga
     1981 agtgtggccg ccggcgtgtt gccggtaagc gagttcaccc gcggtcaacg cggtctcgat
     2041 gggcgggaac tccgtggtat cgagcgcgcg cttacccaac aggcgataca ccggacttgc
     2101 ggcgacg
//
LOCUS       DATDEU010000667         6283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_569673, whole genome shotgun sequence.
ACCESSION   DATDEU010000667 DATDEU010000000
VERSION     DATDEU010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569673"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /gene="thyA"
                     /locus_tag="VIV63_15960"
     CDS             complement(<1..488)
                     /gene="thyA"
                     /locus_tag="VIV63_15960"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY6126148.1"
                     /translation="MKQFLDFMRHVRRNGAEKADRTGTGTLSVFGHQMRFDLAAGFPL
                     VTTKRVHFKSIAYELLWFLRGETNTKFLRENGVSIWDEWADENGELGPVYGKQWRSWP
                     AHDGREIDQISQVVEQLRSNPDSRRIIVSAWNVGELDQMKLMPCHAFFQFYVSDKRLS
                     CQL"
     gene            complement(507..1334)
                     /gene="lgt"
                     /locus_tag="VIV63_15965"
     CDS             complement(507..1334)
                     /gene="lgt"
                     /locus_tag="VIV63_15965"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY6126149.1"
                     /translation="MIPFPTFDKIAFEIGPFGDFGPLKVHWYGIMYLVAFASAWFLAR
                     VRAKKPGSTWKPVDVDDLLFFSMLGVILGGRLGYVFFYGMSFWANDPWYPLKITDGGM
                     SFHGGLIGVMIALAIFAVRRKRRIADVFDFGAPLPGIGLFAGRIGNFINGELWGKPAD
                     VPWAFVYDGVARHPTQLYEAALEGLVLFAILWIYTSKPRPRMAPAGLFLVVYSLARIT
                     VEFWRVPDEHLSYLAGNWVTMGMLLSLPMLLIGLTLMFMAYRRREPSGNFSAVPQPA"
     gene            complement(1389..2582)
                     /locus_tag="VIV63_15970"
     CDS             complement(1389..2582)
                     /locus_tag="VIV63_15970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY6126150.1"
                     /translation="MTNPVLKLKRGEDRRLRAGHLWVFSNEVDTAATPLTQFEPGAAV
                     QVHSDKDQFLGHAYVNPRTLIAARIVGRDPSYPLDGSLLVHRLRIALSLRERLYREPF
                     YRLVFGESDGLPGLVLDRYGDIVVAQSGTAGIDRLRAEIEAAITKVVNARVIVWKNDS
                     GARELEGLERNVSMAGEGEIPAELSIREQGVEFTAPLAEGQKTGWFYDQAANRERLRR
                     YLPPGARVLDVCSYVGAWAVTALKGGAASATCVDSSATALEFVARNARANGVEVETLK
                     ADAFEALKALQESGAKFDVVILDPPAFAKRKKDAPQAQAAYRKLNQLALPLIDRDGLL
                     VSCSCSYHMPAEDLLAAIQTAARHTSRFVQELEQGGQSPDHPVHPAIPETRYLKSFFC
                     RVSRE"
     gene            2624..4690
                     /locus_tag="VIV63_15975"
     CDS             2624..4690
                     /locus_tag="VIV63_15975"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY6126151.1"
                     /translation="MARETSPYLLQHAANPVHWYPWGQEAFDAARASGKPVLLSIGYS
                     ACHWCHVMAHESFEDAATAQLMNELFINIKVDREERPDIDKIYQFAHQVLTQRGGGWP
                     LTMFLTHDDQKPFFGGTYFPDKARYGMPAFSTLLKRVAEYYRDQRDELRAQNDTLMDV
                     FGDLTPAPIDGTAELTAAPLVGAREQLNATFDKRYGGFGDAPKFPHPASIDRLMHHWH
                     STAVTQTPDLHALFMATLTLTRMGEGGLYDHLGGGFSRYSVDQFWMIPHFEKMLYDNG
                     ALLATYAEAALATGDPLFRRIATETGEWMLREMQDASSEGTGGGFYSAYDADSEGHEG
                     KFYVWTRTEVRAALTPLEYSVFSRRFGLDEEPNFEGAWHAHVFVSLDQIAKELSLDPI
                     AIEKQLESARTKLLAIRSKRVWPGLDDKVLSSWNALAIRGLAIAARSLEKPEFAAAAD
                     RALTFVRANLWKDGRLLATAKDGVAHLNAYLDDYAYLANALLEMLQVRWRNEDAAWLR
                     EILDAMLKHFEDGELGGFFFTSDDHETLIHRSKSFSDDAIPAGNGIAARALIRAGYLL
                     GEPRWLDAGERTLRAAWLALNRFPHGHMSLLEALAEYLDPPEIVIVRETSDSGAWQRE
                     LGKLYAPHRMVFSIPAKLEGLDAAIADKKAGSGNRAYICRGSTCSAPVESLADLIRTA
                     QARVQN"
     gene            complement(4687..5085)
                     /locus_tag="VIV63_15980"
     CDS             complement(4687..5085)
                     /locus_tag="VIV63_15980"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY6126152.1"
                     /translation="MAVQAQYLAYILEQLHGITNLRSRPMFGAVGLYSGELFFGLVDD
                     DTLFLKSDSTNSAEYIARKMPRFMPFPDRPEAVMAYYQVPADIIEDAESLRGWARKSV
                     AVALTSHAAKARPAKKAAKKKSPPRRSTRR"
     gene            complement(5088..5576)
                     /locus_tag="VIV63_15985"
     CDS             complement(5088..5576)
                     /locus_tag="VIV63_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY6126153.1"
                     /translation="MDDQSPENSLPFETAIHRRVNEARAGRDNTVLGRCASGWAVFGH
                     QQFVQGYLLLLPDPVVPDLNSLTPERRSQFLLDMSHLGDALVRTTSPLRVNYAIFGNV
                     EPALHAHVIPRYRNEPAHLRTQHPWAYDWNAAPAFDAVTLGPLVKSLRTELAQLGTLR
                     TP"
     gene            5609..>6283
                     /locus_tag="VIV63_15990"
     CDS             5609..>6283
                     /locus_tag="VIV63_15990"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126154.1"
                     /translation="MKFIARLLLISLIAAPVMGAPANDATDADALLWFDANGVTASGA
                     ALLNEMRNAEKYGLRARDYAVDSLLIRAEALAGNPDAGALRILDKDVSARVGRFVTQL
                     HSGRISPRTLGHDLDVAHDTPDSTVAVRALAHSTQPLAVLADYEPAFHHYELLRDALA
                     RYRVLAAQPAPAPLPPSGRPSVKPGERYPAMPALRARLVQLGDLEKSALTLPHEPADS
                     DLLDAVT"
BASE COUNT         1174 a         2010 c         2019 g         1080 t
ORIGIN      
        1 agctggcagg acagtcgctt gtccgacacg tagaactgga agaacgcgtg gcagggcatg
       61 agcttcatct ggtcgagctc gcccacgttc caggcgctga cgatgatgcg gcgcgaatcc
      121 ggattcgaac gcagctgttc aaccacctgc gaaatctggt cgatctcgcg gccatcatgc
      181 gccggccacg aacgccattg cttgccgtac accgggccga gttcgccgtt ttcgtccgcc
      241 cactcgtccc agatgctgac gccattctcg cgcaggaatt tcgtattggt ttcgccgcgc
      301 aggaaccaca gaagttcgta cgcgatggac ttgaagtgca cgcgcttggt ggtgaccagc
      361 ggaaaccccg ccgcgagatc gaagcgcatc tggtggccga acaccgacag agtgccggtg
      421 ccggtacggt ctgccttctc ggcaccgttg cgtcgcacgt gacgcatgaa atcgagaaac
      481 tgtttcatgc cctggccttc acgccactac gccggctgcg gaacggccga gaagtttccc
      541 gaaggctcac ggcggcgata ggccatgaac atgagcgtga gaccgatgag cagcatgggc
      601 aggctcagca acattcccat ggtgacccag ttgccggcga ggtaactcag gtgctcgtcg
      661 ggaacgcgcc agaattcgac cgtgatgcgt gccagcgagt agacgaccag gaacaacccg
      721 gccggagcca tgcgtggcct cggcttcgag gtgtagatcc acaggatggc gaacagcacg
      781 agtccttcga gcgccgcctc gtagagctgc gtggggtgcc gcgcgacgcc gtcgtagacg
      841 aaggcccacg gcacgtcagc gggtttgccc cacaactcgc cgttgatgaa gttgccgatg
      901 cgtcccgcga acagtccgat gcccggcaga ggcgccccaa agtcgaagac atcggcgatg
      961 cggcgcttgc gccggacggc gaatatggcg agcgcgatca tgacgccgat gaggccaccg
     1021 tggaacgaca tgccgccatc ggtgatcttg agcggatacc acggatcgtt cgcccagaac
     1081 gacatcccgt agaaaaacac gtagcccagg cggccgccga ggatgacgcc gagcatgctg
     1141 aagaacagta aatcgtcgac gtcgacgggt ttccacgtgg agccaggctt tttcgcgcga
     1201 acgcgcgcga ggaaccaggc cgacgcgaac gcgacgaggt acatgattcc gtaccagtga
     1261 actttgagcg gtccgaagtc gccgaaaggg ccgatctcga atgcgatttt gtcgaaggtg
     1321 gggaacggga tcattgggcg ggtagtttaa ggaacctgtg atcaatccgc acggtgatcg
     1381 gcgcttccct attcccgcga gacgcggcag aaaaaagatt tcaggtaccg ggtctcggga
     1441 atggccggat gcacggggtg atcgggcgac tgaccgccct gctccagttc ctgcacgaaa
     1501 cggctggtat ggcgcgcggc ggtctggatg gcggcgagga gatcttcggc cggcatgtgg
     1561 taagagcacg aacacgacac cagaagacca tcgcgatcga tgagcggcag cgcgagctgg
     1621 ttcagctttc gatatgcggc ctgtgcttgc ggagcatcct tcttgcgttt cgcgaaggcc
     1681 ggaggatcga ggatgaccac gtcgaacttc gcgccgcttt cctgcagcgc cttcagagct
     1741 tcgaatgcgt cggccttcag ggtttcgacc tcgacgccgt tcgcacgcgc attgcgggcc
     1801 acgaactcga gggccgtcgc cgacgaatcc acgcaggtgg cgctggccgc gccgcccttg
     1861 agcgcggtga ccgcccacgc gcccacgtaa ctgcatacgt cgagcacacg tgcgcctggc
     1921 ggcaggtagc gacgcagccg ttcgcgattc gcggcctggt cgtagaacca gccggttttc
     1981 tgaccttccg cgagtggcgc ggtgaactcc acgccctgtt cgcgaatgga gagttcggcg
     2041 ggaatttcgc cctcgcccgc catcgacacg ttgcgttcga gaccttcgag ttcgcgcgcg
     2101 ccggagtcgt tcttccacac gatcacgcgc gcattcacca ccttggtgat ggccgcttcg
     2161 atctcggcgc gaagccggtc gatgccggcg gtgccgctct gcgcgaccac gatgtcgccg
     2221 taacgatcga gcacgaggcc cggcagtccg tcggattcgc cgaacaccag gcgataaaaa
     2281 ggttcgcgat agagccgctc gcgcagggac agcgcgatgc gcaggcgatg aaccagcagc
     2341 gaaccatcga gtggatagga cggatcgcgt ccgacgatgc gcgcggcgat cagcgtgcgc
     2401 ggattgacgt aggcgtggcc gaggaactgg tccttgtcgg agtgcacctg caccgccgcg
     2461 cccggctcga actgggtcag cggtgtggcg gcggtgtcga cttcattgct gaagacccac
     2521 agatggccgg cgcgaaggcg gcggtcttcg ccgcgcttga gtttgaggac ggggttggtc
     2581 acgggttgta ttctaccgac ccatggatgc caccaagaac agattggctc gtgaaacgag
     2641 cccgtacctt ctgcaacacg cggcaaatcc cgtgcactgg tatccctggg ggcaggaagc
     2701 gttcgatgcg gcccgcgcca gcggcaaacc ggtgttgctg tccatcggtt attccgcatg
     2761 ccactggtgc catgtcatgg cgcacgaatc cttcgaagac gcggccaccg cacagctcat
     2821 gaacgagctg ttcatcaaca tcaaggtgga tcgcgaagag cggcccgaca tcgacaagat
     2881 ctaccagttc gcgcaccagg tactcacgca gcgcggcggc ggctggccgc tcaccatgtt
     2941 cctgacgcac gacgaccaga agccgttttt cggcggcacc tattttccgg acaaggcgcg
     3001 atacggcatg ccggcgtttt cgacgctgct caaacgggtc gccgagtact accgcgatca
     3061 gcgtgacgaa ctgcgcgcac agaacgacac gttgatggat gtcttcggcg acctcacacc
     3121 ggcgcccatc gacggcacgg cggaactgac agcggcgccg ctcgtgggcg cgcgcgaaca
     3181 attgaacgcc acgttcgaca agcggtacgg cggtttcggc gatgcgccga aatttccgca
     3241 tcccgcgtcc atcgaccggc tcatgcatca ctggcattcg accgcggtga cgcagacgcc
     3301 cgacctgcac gcgctgttca tggcgacgct cacgctcacg cgcatgggcg aaggcggcct
     3361 gtacgaccat ttgggcggcg gcttctcacg ctattcggtc gaccagttct ggatgattcc
     3421 gcacttcgag aagatgctgt acgacaacgg cgcgctgctc gcgacctatg cggaggcggc
     3481 gctcgcgacg ggcgatccgc tcttcaggcg catcgccact gaaaccggtg agtggatgct
     3541 gcgcgagatg caggacgcct cgtccgaagg aacggggggc ggcttctact ccgcgtatga
     3601 cgccgattcc gaaggccacg aaggcaagtt ctatgtgtgg acgcgcaccg aggtgcgcgc
     3661 cgcgctgacg ccgctggaat acagcgtgtt cagccgccgc ttcggcctcg acgaagaacc
     3721 caacttcgaa ggcgcgtggc atgcgcacgt gttcgtgtcc ctcgaccaga tcgcgaagga
     3781 gctgtcgctc gaccccatcg ccatcgaaaa acaactggag tccgcgcgga ccaaactgct
     3841 ggccatccgc agcaagcgtg tctggccggg actcgacgac aaggtgttga gcagctggaa
     3901 cgcactcgcg atccgtgggc tggccatcgc cgcccggtcg ctggaaaagc cggagttcgc
     3961 cgccgccgcg gatcgtgcgc tgacgttcgt gcgggccaac ctgtggaaag acggacgcct
     4021 gctggccacg gcgaaggatg gcgtcgcgca cctcaatgcg tacctcgacg actacgccta
     4081 tctcgccaat gcgctgctcg aaatgctgca ggttcgctgg cgcaatgaag atgccgcctg
     4141 gctgcgggaa atcctcgacg ccatgctgaa gcatttcgaa gacggcgagc tcggcggctt
     4201 cttcttcacc tccgacgatc acgaaacgct gatccaccgc agcaagagtt tcagcgacga
     4261 tgcgattccc gccggcaacg gcatcgcggc gcgcgcgctg atccgcgccg gctacttgtt
     4321 aggcgagccg cgctggctgg acgccggcga acgcacgctg cgcgcggcct ggctcgccct
     4381 caatcgcttt ccgcatggcc acatgagcct gctggaggcg ctcgcggaat atctggatcc
     4441 gccagagatc gtgatcgtgc gcgagacctc ggacagcggc gcctggcaac gcgagctcgg
     4501 caagctgtac gcaccgcacc gcatggtgtt ttcgataccc gcgaaactcg agggcctgga
     4561 tgcagccatt gccgacaaga aggctggcag cgggaaccgg gcctacattt gccgcgggtc
     4621 gacctgctcg gcgccggtgg aatctctcgc ggacctcatc cgcaccgcac aggctcgcgt
     4681 acagaactag cggcgtgtcg agcgccgtgg cggggatttc tttttcgccg ccttcttcgc
     4741 cggccgcgct ttcgccgcgt gcgaagtcag cgcaacggcc accgatttcc gcgcccaccc
     4801 gcgcagcgac tcggcatcct cgatgatgtc ggccggtacc tggtaatacg ccatcaccgc
     4861 ctccggccga tccggaaacg gcatgaagcg cggcatcttg cgcgcgatgt actcggcgct
     4921 gttggtgctg tcgctcttca aaaacagcgt gtcgtcatcg acgagaccga aaaacaattc
     4981 gccgctgtag agtccgacgg cgccgaacat ggggcgcgaa cgcagattcg tgattccgtg
     5041 gagctgctcg aggatgtacg cgagatattg cgcttgcacc gccatcgtca gggcgtccgc
     5101 agcgtgccga gttgcgccaa ctcggttcgc aatgacttca cgagcgggcc cagcgtgacg
     5161 gcatcgaacg ccggcgccgc gttccagtcg taggcccacg gatgctgcgt gcgcaggtgc
     5221 gcaggctcgt tgcggtaccg cgggatcacg tgcgcgtgca acgccggctc cacgttgccg
     5281 aagatggcgt agttgacccg cagcggcgag gtggttcgca ccagtgcatc accgaggtgc
     5341 gacatgtcga gcaggaactg cgagcggcgc tcgggcgtca gcgaattcag gtcggggacc
     5401 accgggtccg gcaacagcag cagataaccc tgcacgaact gctggtgacc gaacactgcc
     5461 caccccgaag cgcagcggcc gagcacggtg ttgtcgcggc cggcgcgcgc ctcgttcacg
     5521 cggcgatgga tcgcggtttc gaatggcaag gaattctcgg gggattgatc gtccatacta
     5581 tgtgtcacca ccggcagatt tctcccacat gaaattcatc gctcgattgc tgctcatcag
     5641 tttgattgcc gcgcccgtaa tgggcgcccc ggcaaatgat gcgacggatg ccgacgcgtt
     5701 gctatggttc gatgcgaatg gagtcacggc cagcggcgca gcgttgctca acgagatgcg
     5761 caatgccgag aagtacggac tgcgcgcgcg cgactacgcg gtcgactcgc tgctgatccg
     5821 cgccgaagcg ctcgccggga atcccgatgc cggtgcactg cggattctcg acaaggacgt
     5881 cagtgcgcgt gtcgggcgtt tcgtcacaca gctgcatagc gggcgtatct caccgcgaac
     5941 attgggccac gacctcgacg tcgcgcacga cacgcccgac agcaccgtcg cggtgcgggc
     6001 gctggcgcac agtacccagc cactcgccgt gctcgccgac tatgaacccg cgtttcatca
     6061 ctacgagctg ttgcgcgacg cgcttgcgcg atatcgcgtg ctggcggcac agcccgcacc
     6121 cgcgccgctg ccgccttccg gccggccgag cgtcaaaccc ggtgaacgtt atccggccat
     6181 gcccgccctt cgcgcacggc tggtgcaact cggtgatctc gagaagtccg cgctgacgct
     6241 gccccacgag ccggccgact ccgacctgtt agacgccgtc acc
//
LOCUS       DATDEU010000668         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_275247, whole genome shotgun sequence.
ACCESSION   DATDEU010000668 DATDEU010000000
VERSION     DATDEU010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275247"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..804)
                     /locus_tag="VIV63_15995"
     CDS             complement(238..804)
                     /locus_tag="VIV63_15995"
                     /inference="COORDINATES: protein motif:HMM:NF028133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6126155.1"
                     /translation="MYDGKDTIVIFDKLAYEDLLPVRWRPSVQLVDDVTAQHYAERNI
                     RVLQACDALEEHGQLDKSDDESPHSADLRRLDFKLNLLLDLAGQLLAASQPRLRPSSI
                     RLNAMGASFTYDEGLVEGAYGVLELMLRDIVVQPLHLPAQVVSGAPAGQVRVRFLSLG
                     EVVADHIEKLVFRRHRRKIAGARGIRRA"
     gene            complement(834..1163)
                     /locus_tag="VIV63_16000"
     CDS             complement(834..1163)
                     /locus_tag="VIV63_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126156.1"
                     /translation="MSTSASQTARMRPHLRLVSSTLAPVSPAAADCGWRRVAAAIVAC
                     TWDATRYMRDQRWGRVEEELTDRRELLGLMRSMELDANGRSCLRSLDEAALESERAIA
                     TMMGKSR"
     gene            complement(1160..1573)
                     /gene="fliS"
                     /locus_tag="VIV63_16005"
     CDS             complement(1160..1573)
                     /gene="fliS"
                     /locus_tag="VIV63_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEY6126157.1"
                     /translation="MAFSPNAGKLDAYRSTAVHSGVDAADPHRLVVMLMDGALERIAA
                     ASGMMKHGGGVEKAQLLHRTVAIIDELRNGLNLKAGGAIATNLDALYEYMCQRIMQAN
                     AQNKPEWLDEVSRLLGEIRSAWLSIPQSRGANPPR"
     gene            1710..2162
                     /locus_tag="VIV63_16010"
     CDS             1710..2162
                     /locus_tag="VIV63_16010"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEY6126158.1"
                     /translation="MPSHDPKLCREQLATLMAEQNVHLAALEGLLTQEYDLLQTRDAE
                     GLEKAGTARQQCIGHIVRIEDERRALCRATGRDEDSAGLHGLLAWCDPTGLLVPAMQE
                     YRERTQRCRELNDRNGILVNGRLQQDARTYGPGSDESGGYGRKLTTRA"
     gene            complement(2181..>3142)
                     /gene="fliD"
                     /locus_tag="VIV63_16015"
     CDS             complement(2181..>3142)
                     /gene="fliD"
                     /locus_tag="VIV63_16015"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEY6126159.1"
                     /translation="GDSSFSIEVGEGGNSVANIRDAINKSAANKGVQASLLTDVEGTH
                     LVLTSTKPGEDYALTVTASGGNGGLEQLETMEVRSVAQDAILFVSGYEIHSSSNTVSN
                     AIEGVTLTLKAPTEDGSTVLLGVERDDAAIQDAAKKFVDAYNSLAGTIKSLSRYDVST
                     EAAGALLGDAMLRNIDSQVRKLISTPVAGVNGNYNTLASLGITTQTDGKLKLDTVRFN
                     AALSANPGAVSDIFTSSNGVAVKLADFMGSKTSVTGEVTVRSSNITDTLVDLKDQQDS
                     LNARMKIIEQRYFRQFNALDTLLGQMNNTAGALDSWLKQSDRD"
BASE COUNT          547 a         1004 c          994 g          597 t
ORIGIN      
        1 cacggcgcgc gactccctcg ctctgcgcac tgatgcattc gattggctgc acggtcactc
       61 cttacgtctc tcgcgagtat cttcgcgcca atgctgcgcg agaaagcgtg acgcgccgtg
      121 catcttcgca gacacgagag agaaatgacc taacaacttt gcgcgagctg ccgatgactg
      181 cggcgcgcga gggaatccct tactgataac tccctagtgt tttttcccgc gaacgagtca
      241 tgcgcgacga attccgcgcg cgccggcaat ctttcgccgg tgccggcgaa acaccagctt
      301 ctcgatgtga tcggcgacca cttcgccgag cgagagaaaa cgcacgcgga cttgtccggc
      361 cggcgcgccg gagaccactt gcgcgggaag atgcagcggc tggacgacga tgtcgcgcaa
      421 catgagctcg agcacgccgt atgcgccttc gaccagacct tcgtcgtaag tgaaactcgc
      481 acccattgca ttcaggcgga tgctcgacgg ccgcagacgc ggctgactcg cggcgagaag
      541 ctgtccggcg aggtcgagca gcaggttcaa tttgaagtcg aggcggcgca gatcggccga
      601 gtgcggcgat tcgtcgtcgg acttgtcgag ctgcccgtgt tcttcgagcg catcgcaggc
      661 ctggagcacg cggatattgc gctcggcgta atgctgggcg gtgacgtcgt cgaccagttg
      721 cacggacggc cgccagcgaa ccggcagcag gtcctcatag gccagcttgt cgaatatgac
      781 gattgtgtct ttgccgtcgt acacgcgtta cctcgcgttg acccgcggcg agtctagcgg
      841 cttttgccca tcatggtggc aatggcgcgt tcggattcga gcgccgcttc atcgagcgag
      901 cgcaggcagg agcggccgtt ggcatcgagt tccatcgaac gcatgagacc caggagctcg
      961 cggcgatcgg tcagctcttc ttccaccctg ccccagcgct ggtcgcgcat gtagcgcgtc
     1021 gcgtcccagg tgcaggcgac gatggccgcg gccacgcggc gccagccgca gtctgctgcc
     1081 gccggcgata ccggcgccag cgtgctgctc accaggcgca ggtgcggccg catgcgcgca
     1141 gtctgtgagg cggacgtgct catctcggcg gattcgcgcc gcgcgactgc gggatcgaca
     1201 gccaggccga gcggatctcg cccagcaacc ggctgacttc gtctaaccac tcgggcttgt
     1261 tctgggcatt ggcctgcatg atgcgctggc acatgtattc gtagagcgcg tcaaggttgg
     1321 tggcgatggc gccgcccgcc tttaaattga ggccgttgcg aagctcgtcg atgatggcca
     1381 ccgtacgatg cagtagttgg gccttctcga cgccaccacc gtgcttcatc atgccgctcg
     1441 cagcagcgat gcgttcgagc gcgccgtcca tcaacatcac gacgaggcga tgcggatcgg
     1501 cggcgtcaac gcccgagtgc acggcggtgc tgcggtaggc gtcgagtttt ccggcattgg
     1561 gtgaaaaggc catcgcgatc tgctccaaac tacgttgtgt agccaggaac ttaacggccc
     1621 cgcgggggcg gacttgaggg tacgatagtg cgaagcgggt ccgcctcgcc gcaagtcatc
     1681 tgacaaataa tcctgccagg tacaagccca tgccatctca cgatcccaag ctctgccgcg
     1741 aacagctcgc caccctgatg gctgagcaga acgttcacct tgccgcactc gagggcctgc
     1801 tgacgcagga gtacgacctg ctgcagaccc gcgacgccga aggcctcgaa aaggccggca
     1861 cggcgcgcca gcagtgcatc ggccacatcg tgcgcatcga ggacgaacgc cgcgcgctgt
     1921 gccgcgcaac cggtcgcgac gaagactcgg ccgggctgca cggcctgctc gcctggtgcg
     1981 accccaccgg gctgctggtg ccggcgatgc aggaataccg cgagcgcacc cagcgttgcc
     2041 gtgaactgaa cgatcgcaat ggcatcctgg tcaacggcag actgcagcag gatgcgcgca
     2101 cttacgggcc cggcagcgac gagagtggcg gctacggccg caagctcacc acgcgcgcct
     2161 gatcggccgc ggtcatcgcc tcagtcccga tcgctctgct tcaaccagct gtcgagtgcg
     2221 ccagcggtgt tgttcatctg gcccaacaac gtgtcgagcg cgttgaactg cctgaagtag
     2281 cgctgctcga tgatcttcat gcgcgcattc agcgagtcct gctgatcctt caggtctacc
     2341 agcgtgtcgg tgatgttgct gctgcggacc gtcacttcgc ccgtgaccga ggtcttcgac
     2401 cccatgaagt cggcaagctt gaccgcaacg ccgttgctgg acgtgaaaat gtcgctgacc
     2461 gcgccagggt tggccgaaag agccgcgttg aatctgacgg tgtcgagctt cagcttgcca
     2521 tctgtttggg tcgtgatccc taagctcgca agcgtgttgt agttgccgtt cacgccagcc
     2581 accggcgtgg aaatcagctt gcgcacctgt gagtcgatgt tacgcaacat ggcatcgccg
     2641 agcagcgcgc cggccgcctc ggtggagacg tcatacctgc tcagggactt gatggtgccc
     2701 gccagcgagt tgtaggcatc cacgaatttc ttggctgcgt cctggatcgc cgcgtcgtca
     2761 cgctcgacgc ccagcaggac cgtgctgcca tcttcggtgg gagccttcag cgtcagcgtc
     2821 acgccttcga tggcgttgct gacggtgttc gaagagctgt ggatctcata acccgacacg
     2881 aacagaatgg cgtcctgcgc cacgctgcgc acttccatgg tctcgagttg ttccagcccg
     2941 ccgtttccac cgctggcggt cacggtcagc gcgtagtcct cgccgggttt cgtgctggtg
     3001 agcaccaggt gcgtgccttc cacgtcggtg agcaacgatg cctgcacgcc cttgttggcg
     3061 gcggacttgt tgattgcatc gcgaatgttg gcgacgctgt tgccgccttc acccacctcg
     3121 atgctgaaag aggaatctcc ca
//
LOCUS       DATDEU010000669         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_275448, whole genome shotgun sequence.
ACCESSION   DATDEU010000669 DATDEU010000000
VERSION     DATDEU010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275448"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /gene="mutY"
                     /locus_tag="VIV63_16020"
     CDS             <1..959
                     /gene="mutY"
                     /locus_tag="VIV63_16020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY6126160.1"
                     /translation="WVSEIMLQQTQVATVIPYFERFMKRFPDVRSLADAPADEVLHLW
                     TGLGYYARARNLHRAAQVVRDEHDGVFPSGIEAVMALPGIGRSTAGAILAISAGIRHP
                     ILDGNVKRVLARFYAVDGAPDESATLETLWRLADDNTPANDVAAYTQAIMDLGATLCT
                     RGKPRCDVCPVAADCRARIEGRQSELPAAKRKRAVRRQRHAFMLVARRATEVLLVQRP
                     PSGIWGGLWCLPEFDHRDAAEQFAVTTLANATLARAALPDIAHSFTHFDLVITPVVAK
                     CRGDARVRDGTALWYDLTHPARVGLPAPIKTLLGTLLERNES"
     gene            956..1225
                     /locus_tag="VIV63_16025"
     CDS             956..1225
                     /locus_tag="VIV63_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY6126161.1"
                     /translation="MTRMVNCILLKKEAPGLDRPPYPGELGKRIFENVSKEAWQQWVK
                     HQVMLINEYRLTPIDPAHRKRLEFEMEKFFFGEGSQAPKEFVKPD"
     gene            complement(1237..1701)
                     /locus_tag="VIV63_16030"
     CDS             complement(1237..1701)
                     /locus_tag="VIV63_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126162.1"
                     /translation="MRSRLAPLLLLLPLWHSSSFAQCAPAPDSPYFFRNLAEQRAEAR
                     LASDRSFYESLLSDHFADKENFIAGELAMHHDASRKGFYAVRNFSLVEHRKGLAVTSY
                     LLVEGDIGEGKSNARETWQREVFEVENGKWRLVSIEDAEPRPNQQAQAPPAG"
     gene            complement(1719..2582)
                     /locus_tag="VIV63_16035"
     CDS             complement(1719..2582)
                     /locus_tag="VIV63_16035"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY6126163.1"
                     /translation="MNTAAKLATTPRAVIQTLRGQPASDGAGVKLTRVIGQPKLPDLD
                     PFLLLDEFGTDKAEDYIAGFPEHPHRGFETVTYMLDGRMRHKDNHGNEGVLEPGAVQW
                     MTAGRGIVHSEMPEQREGRMRGFQLWMNLPAKDKMTAPNYQEFGGDKLPVVERPGVSV
                     KVIAGELDGATGPVRQPATDPVYMDIRLDAGVDFALPLPVGHSAFVYVYEGGVSVGVS
                     REAATIHAQELAVLGDGSEVRLQGREAKSRLILVAGRPLREPVARYGPFVMNTREELH
                     QAFADFQAGKF"
BASE COUNT          458 a          839 c          857 g          462 t
ORIGIN      
        1 tgtgggtttc cgaaatcatg ctgcagcaga cgcaggtggc cacggtcatc ccctatttcg
       61 aacgcttcat gaaacgtttt cccgacgtgc gctcgctcgc cgatgcgccg gcggacgaag
      121 tgctgcattt gtggaccggg cttggctact acgcccgcgc gcgcaatctg catcgcgcgg
      181 cccaggtcgt gcgcgacgag cacgacggag tgtttccgtc tggcatcgaa gcggtgatgg
      241 cgctgccggg aatcggccgc tcgacggccg gcgctattct cgcgatctcc gcgggcatcc
      301 ggcatccgat cctggatggc aacgtgaagc gggttctcgc gcgcttctac gccgtcgatg
      361 gcgcgcctga tgaaagcgcc acgctcgaaa cactctggcg gcttgccgac gacaacacgc
      421 cggcgaacga cgtcgccgct tacacccagg ccatcatgga tctgggagcc acgctgtgca
      481 cacgcggcaa gccgcgttgt gatgtctgcc ccgtcgccgc cgactgccgc gcgcgcatcg
      541 aggggcggca gagtgagctg ccggccgcga agcgcaaacg cgccgtacga cgacagcggc
      601 atgcgttcat gctggtggcg cgccgggcga cggaagtatt gctggtgcag cgcccgccga
      661 gcggaatctg gggcggcctc tggtgcctgc ccgagttcga tcatcgtgac gcggcggaac
      721 agttcgccgt caccacgctc gcgaatgcga cgctggcgcg tgcggcactg cccgacattg
      781 cgcacagctt cacgcacttc gacctcgtga tcacgcccgt cgtcgcgaag tgccgtggcg
      841 atgcgcgcgt gcgcgacggc accgcgctgt ggtatgacct cacgcatccc gcgcgcgtcg
      901 ggctgcccgc acccatcaag acactgctcg gaaccttgtt agagagaaac gaatcatgac
      961 gcggatggtg aactgcattt tgttgaagaa agaggcgccc ggcctcgacc ggccgcccta
     1021 tccgggcgag ctcggcaagc gcattttcga aaacgtgtcg aaagaagcgt ggcagcagtg
     1081 ggtgaagcac caggtcatgc tcatcaacga gtaccggctg acgccgatcg accccgcgca
     1141 ccgcaagcgc ctggagttcg agatggagaa gttcttcttc ggcgagggct cgcaggcgcc
     1201 gaaggaattc gtgaagccgg actgacgggg tgcgcgctag ccggctggcg gcgcctgcgc
     1261 ctgctgattc ggccgtggct cggcgtcctc gatggaaacg aggcgccact tgccgttctc
     1321 cacttcgaag acttcccgct gccaggtctc ccgggcgttg gatttgccct cgccgatgtc
     1381 gccctcgacg agcaggtagc tggtgacggc gagacccttg cggtgttcga ccaggctgaa
     1441 gttcctgacg gcgtagaagc ctttgcgact cgcgtcgtga tgcatcgcca gctcaccggc
     1501 gataaagttt tccttgtcgg caaaatgatc gctgagcagg gattcataga agctgcgatc
     1561 ggaagccagc ctcgcttcgg cacgctgttc ggccagattg cggaagaagt acggtgaatc
     1621 gggcgccggc gcgcattgcg cgaaggatga cgagtgccac aaaggcagca gcagcaacag
     1681 gggcgccagg cgtgatcgca tggcgcccat catacaactc agaacttgcc ggcctgaaaa
     1741 tccgcgaacg cctggtggag ctcttcgcga gtgttcatca cgaaggggcc atagcgcgcc
     1801 accggttcac gcagcgggcg gcccgcgacg agaatcagtc tgcttttcgc ttcgcgaccc
     1861 tgcagacgca cttcgctgcc atcgccgagc accgcgagtt cctgtgcatg gatggtcgcc
     1921 gcttcgcgcg aaacgcccac cgacacaccg ccctcgtaga cgtacacgaa agccgaatgg
     1981 cctaccggca agggcagcgc gaaatccacg cccgcgtcga gccggatgtc catataaaca
     2041 gggtccgtcg ccggctggcg cacggggccg gtggcgccat cgagctcgcc ggcgatcacc
     2101 ttgacgctca cgccgggccg ttcgaccacc ggcagcttgt caccgccgaa ctcctgatag
     2161 ttaggcgccg tcatcttgtc cttcgcgggc agattcatcc acagctggaa gccgcgcatg
     2221 cggccctcgc gctgctcggg catttccgag tgcacgattc cgcggccggc agtcatccac
     2281 tgcacggcgc ccggctcgag cacgccttcg ttgccgtggt tgtccttgtg gcgcatgcgg
     2341 ccatcgagca tgtatgtgac cgtttcgaaa ccccgatgag ggtgctccgg aaacccggcg
     2401 atgtagtcct cggctttgtc cgtgccgaat tcatcgagca gcaggaaggg gtcgagatcg
     2461 ggcagcttgg gctggccgat gacacgcgtg agcttgacgc ccgcgccgtc cgaagcgggc
     2521 tggccgcgaa gcgtctggat gactgcgcgc ggggtggttg cgagtttggc agcggtattc
     2581 atggtcggcc cgaaatggtg tcaatgcgtc gcaaat
//
LOCUS       DATDEU010000670         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_714475, whole genome shotgun sequence.
ACCESSION   DATDEU010000670 DATDEU010000000
VERSION     DATDEU010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714475"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VIV63_16040"
     CDS             complement(<1..1017)
                     /locus_tag="VIV63_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY6126164.1"
                     /translation="MNLATLDIAILAIYAVGIFVLAQWVSREKGEHKKDAQDYFLASR
                     SLPWWAIGTSLIAANISAEQIVGMSGSGYVMGMAIASYEWMAALTLIVVGKWILPVFL
                     KNGIYTMPEFLEKRYSPGVRTVMAIFWLVLYIFVNLTSILWLGATAVNTITGVDIDVA
                     IIALGVFALVYALYGGLKAVALTDIVQVTLLVLGGLVITWIALTKVGDGGGVIAGFTR
                     LTTELPEKFDMILSADNPSYKALPGLAVLLGGMWVMNVSYWGFNQYIIQRGLAAKSVN
                     EAQKGIVLAAFLKLLMPAIIVLPGIAAILLTPGLERPDQAYPSLMAMLPKGILGLVFA
                     ALVAA"
     gene            complement(1054..1680)
                     /locus_tag="VIV63_16045"
     CDS             complement(1054..1680)
                     /locus_tag="VIV63_16045"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEY6126165.1"
                     /translation="MYFGRTDIELVAILRGLTPARALEVGAALTDSGFRTIEVPLNSP
                     DPFDTIRLLAQAHGDSCLIGAGTVLTPAEVDRVHGAGGRLVVSPNCDGDVIRRALDLG
                     LRVLPGIATATEAFQALRHGATELKLFPASSYGAGHLKALKSVLPRHAKIFPVGGIGS
                     QDIATWLASGADGFGFGGELFKPAYTLAELTKRAQELHQALRVAMEKN"
     gene            complement(1661..>2336)
                     /locus_tag="VIV63_16050"
     CDS             complement(1661..>2336)
                     /locus_tag="VIV63_16050"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008671 - 2-dehydro-3-deoxygalactonokinase
                     activity [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY6126166.1"
                     /translation="LSAPADLYELADDAVSPRDGVSIVRGMSCTNPLGAPDVMRGEET
                     QLFGAQSVLGVMKAGKQLVCMPGTHTKWVSLHEGVVQEFLTAPTGELFAMLCEHSVLV
                     RDKTTPIEHHPRDFERGLAESAKHPEVQVLHRLFQSRSLRLDKQLSPEGAASWMSGLL
                     IGTDVQGALSLFTEHDSAAPVYVIGAPQLTASYALALARSGRTAVQIDGTDAALAGLS
                     HVFREN"
BASE COUNT          445 a          755 c          727 g          409 t
ORIGIN      
        1 cgccgccacc agcgccgcga acaccagccc gaggattcct ttcggcaaca tggccatgag
       61 gctcggataa gcctggtccg gacgctccag ccccggcgtc agcaggatcg cggcgatgcc
      121 gggcagcacg atgatggccg gcatcagcag tttgagaaag gcggcgagca cgatgccctt
      181 ctgtgcctcg ttcacgctct tggcggccaa tccacgctgg atgatgtact gattgaagcc
      241 ccagtaggac acgttcatca cccacatgcc tcccagcagc acggcaagcc ccggcagcgc
      301 cttgtagcta ggattgtcag ccgagaggat catgtcgaat ttttccggta gctcggtggt
      361 caggcgcgtg aagccggcga tgaccccgcc accgtcaccg actttggtca acgctatcca
      421 ggtgatgacg agaccgccga gcaccagcag agtcacctgc acgatgtccg tgagcgccac
      481 tgccttgagg ccgccgtaca gcgcgtagac cagcgcaaac acgccgaggg caatgatggc
      541 cacgtcgatg tcgacgccgg tgatggtgtt caccgccgta gcgcccagcc acaagatgga
      601 tgtgaggttg acgaagatgt acagaaccag ccagaagatg gccatcaccg tgcgcacgcc
      661 cgggctgtaa cgcttctcca ggaattctgg catggtgtag atgccgttct tgaggaacac
      721 cggcaggatc cacttgccga cgacaatcag cgtgagcgcg gccatccact cgtacgacgc
      781 gatggccatg cccatcacgt atccggaacc cgacatgccg acgatctgtt ccgccgagat
      841 attcgccgcg atcagcgatg tgccgatggc ccaccagggc agcgagcgac tggccagaaa
      901 gtagtcctgc gcatctttct tgtgttcgcc tttttcgcgc gacacccact gcgccagcac
      961 aaaaattccg accgcgtaga tcgccaggat ggcgatatcc agagttgcca gattcacgtt
     1021 gacccccaga ggctgcggcg ccatcgggcg ccgttagttt ttttccatcg caacgcgcaa
     1081 ggcctgatgc agctcctgcg ctcgtttcgt cagctccgcg agtgtatacg cgggtttgaa
     1141 aagttcaccg ccaaatccga agccatccgc gcctgacgcg agccatgtgg cgatgtcttg
     1201 cgagccaata ccaccgaccg gaaaaatctt cgcgtgcctg ggaagcacgg atttgagcgc
     1261 tttgagatga cctgcgccgt aactcgatgc gggaaagagt ttgagctcgg tggcgccgtg
     1321 acgcagcgcc tgaaaggctt cggtggcggt agcgatacca ggcagcacgc gcaacccgag
     1381 atcgagtgca cggcggatca cgtcgccgtc gcagttcggg gataccacca gccggccgcc
     1441 cgccccgtgc accctatcta cttcggcggg cgtgagcacc gtcccggcac cgatcaggca
     1501 ggagtcgccg tgtgcctgcg ccagaagcct gatggtgtcg aaggggtccg gcgagttgag
     1561 cggaacctcg atggtgcgaa agccggaatc cgtcagtgca gcgcccactt ccagcgcgcg
     1621 cgccggcgtg aggccgcgca ggatggcaac gagttcgatg tcagttctcc cgaaatacat
     1681 ggctgagccc cgccagcgcg gcgtcggtcc cgtctatctg cacggccgtg cgcccgctgc
     1741 gcgcgagcgc gagagcgtag gaggcggtga gctgcggcgc gccgatgacg tacaccggcg
     1801 ccgcggaatc gtgctcggtg aacaacgaca acgcgccttg cacatccgtg ccgatgagca
     1861 ggccggacat ccacgatgcc gcgccttcgg gcgaaagttg tttgtcgagc cgcagacttc
     1921 ggctctggaa cagccggtgc agcacctgga cctccgggtg cttcgccgat tccgcgaggc
     1981 cccgctcgaa atcccgtggg tgatgttcga tgggcgtcgt cttgtcgcgc accagcacgc
     2041 tgtgttcgca gagcatcgcg aacaactcac ccgtgggcgc cgtcaggaat tcctgcacca
     2101 cgccctcgtg cagcgagacc cacttggtgt gtgtgcccgg catgcagacc agttgcttgc
     2161 cggctttcat gacgccgagc acgctttgcg cgccgaacaa ctgcgtttcc tcgccacgca
     2221 tgacgtcggg cgcgcccagg ggattcgtgc aactcatgcc gcgtacgatg ctcacgccgt
     2281 cgcgtgggct caccgcgtca tcggcgagct cgtaaagatc ggcgggcgct gataga
//
LOCUS       DATDEU010000671         6341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1297774, whole genome shotgun sequence.
ACCESSION   DATDEU010000671 DATDEU010000000
VERSION     DATDEU010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297774"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="VIV63_16055"
     CDS             <1..2073
                     /locus_tag="VIV63_16055"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6126167.1"
                     /translation="VFVGYWQIRRQDRLASVFAVFAAITFFVPAIIMYSQGPGDTMAV
                     VAHFVRVTGELYLLLTLTQMGAADTAQRLRAERELKASNEALEMRVADRTAELQAANS
                     GLRAEAQTRQIAEQRALVQVERLNLLERITHAIAERQDLESIFQVVVRSVEEHMPADF
                     AALCNYSHHSKILTVSRVGTRSGPLALELAMTEHSRIEIDQNGLSRCVAGTLVYEPDI
                     KAIHFPFPQRLARGGLGSMVVVPLMVEQRSGVFGVLVVARRAANAFNSAECEFLRQLC
                     DHVSLAANQAQLHESLKQAYDDLQVTQGAVMQQERLRALGQMASGIAHDINNAISPVS
                     LYVDALLTHETGFSDRARKQLEIIQRAVDDVAHTVSRMGEFYRQGKGYGQMELSPVSV
                     TQAFREVLELTRARWSDMAQKRGAVIETKIECEGESPIVMALASELREALINLILNAT
                     DAMPDGGLLTLRAGHALEAGKGVSRRVFIEVSDTGAGMDEETRRRCLEPFFTTKGDRG
                     SGLGLAMVYGIAQRHGADVDIRSAPGEGTTFRMTFPLTSPAAQSTGSLRIQKIPRHTK
                     ILLIDDDPVLLTSLREVLVHDGHQVQTATGGRVGIEAFLDALKAGKPFPVVITDLGMP
                     HVDGRAVAAAIKAAAPATSVIMLTGWGQRLVASGEIPADVVAVVSKPPRIAELRQVIA
                     ECTTPPGE"
     gene            complement(2075..4207)
                     /locus_tag="VIV63_16060"
     CDS             complement(2075..4207)
                     /locus_tag="VIV63_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126168.1"
                     /translation="MSTLREKLEAQLQRFDDDAFAALGNRGLLRRARKDLEKGAAEIV
                     EETPALLVLKVGEHRIRFDARGAAHAECSCPASGVCQHILAAAITLQAASPKGGITAA
                     TDVPAAATPADAPAAPALDQLRAALLAMTPRELAKHAGKAGYRWAWQFVQDLNVETDL
                     TLSGERNVVIAFRHPRLVFRYMGGPLDGMLADTDIKQLEKYRAAAVMAYQRANGVPLT
                     APEGPAPQDGKLDLGKDHAGAETASASLQESRTRLCAAVRQLACECVTLGLSHFSPGI
                     QERFSTLAVWAQGAQCYRLALLLRRLADHVEMLLERAGGADEHRLFDELTIAYALVAA
                     IESAAARGSAPAPLIGRARNEYEDAGQIELSGLGSLAWRSASGYIGLTMLFWSPKDKT
                     FLTCGDARPEVQRGFDPVARYKASGPWSGLGAPALTTGRRVMLTGALLSAAGRISGSE
                     KTTATIMPRDAAQFIASLDTQSRWSELARIRAAARRSLLAEAQPMKDWVVLRPSKFQR
                     AEFDGTRQTLTWNLVDDEGDVLRAEVAYHPYSAPAIERLEQMDMNSLAPGTLVVAKLR
                     GGSNNFIAEPLSLVRPVTGDEVVVDSLYFDAAPKSGFASRILSKLLRRTDTTTGVLPV
                     MPSATPPVLTDLRHWLCSQAERGVAWDAAAQARSELAARAARLSGAGFTAFRGVGSSA
                     DAAADLLRAQYLCLQYEHLLDDGESEAA"
     gene            complement(4204..5325)
                     /locus_tag="VIV63_16065"
     CDS             complement(4204..5325)
                     /locus_tag="VIV63_16065"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6126169.1"
                     /translation="MKDLTRWRLVLGKYAERRMSGGTGGMQGQDRRRDQALDYLYGRE
                     YEGRGLRKEIAPGSLDPSQLQLVTWLNEVRELFPQDTVEVIEKHALDRYGLTELVTDP
                     KTLQRLEPNQQLLRTLLTLRGHLKGEVLDVARKIIRKVIEEIRRKLEPEVRQTLAGKL
                     NRFRHSPMAVAQNFDAPGTVRRNLKHYDVERRKLVIEELRFFERNARRLPWHIVLCID
                     QSGSMADSVIHSAVMAGILAGLPAFRVKLVVFDTQVVDLSDHVDDPVEILMKVQLGGG
                     TDIAQAMRYCAQIIENPQRTILVLVTDFCEGAPAGELVRAVRALAEARVKLLGLAALD
                     GQAHPVYDRQMAEKLAACGMEIAALTPQRLAHWLVKAIS"
     gene            complement(5322..>6341)
                     /locus_tag="VIV63_16070"
     CDS             complement(5322..>6341)
                     /locus_tag="VIV63_16070"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HEY6126170.1"
                     /translation="GTGLDRLQEHWEYTFTAATEASLVEAAVHGVTVPLAVADRFRAL
                     LDSLVAGAEARDARAAASLLARGCVLGLHDHLPRVLDVLRGAIGADPSFDSVALATGS
                     LGMLWESREPLEARDVAELPVVLRAAYERAIYLGSNLRGMPAGSASRGNDTMHALARL
                     RELLVSQAGAELDASLYWDMVRQLAESHDEPLIRGTAVGLLYSAGNYSAAELANAMQG
                     HFIGITDAQRSVGFLRGLLHAARDAAWQQPELLTSLDTLLSGWDEAQFIAVLPDLRLA
                     FAAMTPKETDRVAEAVAALHGEKTIGPLMNYDVGEGQLRANLALSGTLLEVLEGDGLA
                     AWGKP"
BASE COUNT         1017 a         2139 c         2161 g         1024 t
ORIGIN      
        1 gtcttcgtcg gttattggca gattcgccgg caagaccgcc tcgcgagcgt gttcgccgtg
       61 ttcgcggcga tcaccttttt cgttccggcg atcatcatgt actcgcaggg gcctggagac
      121 accatggccg tcgtcgcgca cttcgtgcgt gtcacgggcg agctctacct gctgctcacg
      181 ctcacgcaaa tgggcgcggc cgacaccgca caacgcttgc gcgccgagcg ggagctcaag
      241 gccagcaacg aagcgctgga gatgcgcgtc gccgatcgca ccgccgaact gcaggccgcc
      301 aacagcggcc ttcgcgccga ggcgcagacc cggcagatcg ccgaacagcg cgcgctggtg
      361 caggtcgagc gactgaacct gctggagcgg atcacacacg ccatcgccga acggcaggat
      421 ctcgaaagca tcttccaggt ggtcgtgcgg agcgtcgaag agcacatgcc cgcggacttc
      481 gccgcgctgt gcaactacag ccatcactcg aagatcctca cggtcagccg cgtgggcacg
      541 cgcagcggac cgctggcgct cgagctggcg atgaccgagc attcgcgtat cgagatcgac
      601 cagaacggac tgtcgcgctg cgtcgccggc acgctggtct acgaaccgga catcaaggcg
      661 attcacttcc cgtttccaca gcgcctggcg cggggaggtc tggggtccat ggtcgtcgtg
      721 ccgctcatgg tggagcagcg cagcggggta ttcggcgtgc tggtggtggc gcgccgcgcg
      781 gccaacgcgt tcaacagcgc cgagtgcgag ttcctgcgtc agctctgtga ccacgtgtcg
      841 ctggcggcca accaggcgca gcttcatgaa tcgctgaagc aggcttacga cgacctgcag
      901 gtcacgcagg gcgccgtcat gcagcaggag cggctgcgcg cgctcggcca gatggcgagc
      961 ggcatcgcgc acgacatcaa caacgccatc tcgcccgtgt cgctgtacgt cgatgcgctg
     1021 ctcacgcacg aaaccggctt cagcgaccgc gcgcgcaaac aactcgaaat catccagcgc
     1081 gccgtcgacg acgtcgccca caccgtctcg cgcatgggcg agttttatcg ccagggcaag
     1141 ggttacgggc agatggaact gtcgccggtc tcggttacgc aggcgtttcg cgaagtcctc
     1201 gagctcaccc gcgcgcgatg gagcgacatg gcgcagaagc gcggcgcggt catcgagacg
     1261 aagatcgagt gcgagggcga gagcccgatc gtcatggcgc tcgccagcga attgcgcgag
     1321 gcgttgatca atctcatcct caatgcaacc gatgcgatgc ccgatggcgg cctgctcacg
     1381 ttgcgcgcgg gtcatgcgct cgaagccggc aagggtgttt cgcgccgcgt gttcatcgaa
     1441 gtcagcgaca ccggcgcggg catggacgaa gaaacgcgcc gccgttgcct ggagccgttc
     1501 ttcaccacca agggcgatcg cggctcgggc ctcggactcg cgatggtgta cggcatcgcc
     1561 cagcgccacg gcgccgatgt cgacatccgg agcgcgccgg gcgagggcac cacgttcagg
     1621 atgaccttcc cgctgacatc gcccgcggcg cagtcgacgg gcagcctgcg catccagaag
     1681 attccgcgcc acacgaagat cctgctcatc gacgacgatc ccgtgctgct cacgtcgctg
     1741 cgcgaagtcc tggtgcacga cggccaccag gtgcagacgg ccaccggcgg tcgcgtcggc
     1801 atcgaggcat tcctcgatgc gctgaaggca ggcaaaccgt ttccggtggt tatcaccgat
     1861 ctgggaatgc cgcatgtcga cggtcgcgcc gtcgcggccg ccatcaaggc ggcggcgcct
     1921 gcgacctccg tcatcatgct cacgggttgg ggtcagcggc tggtcgcgtc gggtgaaatc
     1981 ccggccgatg tcgtcgccgt ggtcagcaag ccgccgagaa tcgcggagct ccggcaggtc
     2041 atcgcggaat gcaccacgcc gcccggcgag tagctcaggc cgcttcgctc tccccatcgt
     2101 cgagcaggtg ttcgtactga aggcacaggt actgcgcgcg cagcagatcc gctgctgcat
     2161 cagcggatga acccacgcca cggaacgcgg tgaatcccgc gccgctcagg cgcgcggccc
     2221 gcgcggcgag ttcgctgcgc gcctgcgcgg ccgcgtccca ggccacgcct cgttcggcct
     2281 ggctgcagag ccagtggcga aggtcggtca acaccggcgg cgtcgccgag ggcatcaccg
     2341 gcagaactcc cgtcgtcgtg tcggtgcgcc ggagcagctt cgagagtatc cggctcgcga
     2401 agccggactt gggtgccgca tcgaagtaaa gggagtccac caccacttca tcacccgtga
     2461 ccgggcgcac caggctcagc ggttcggcga tgaagttgtt ggatcccccg cgcagtttcg
     2521 ccaccacgag cgtgcccggg gccagcgagt tcatgtccat ctgctcgagc cgttcgatgg
     2581 ctggcgcgct gtaggggtga taggccacct cggcacgcaa cacgtcgcct tcgtcgtcga
     2641 ccaggttcca ggtcagtgtc tggcgagtac cgtcgaactc ggcgcgctgg aatttcgagg
     2701 ggcgcagcac cacccagtcc ttcatcggct gggcctctgc cagaaggctg cggcgagcgg
     2761 cggcacgaat gcgcgccagt tccgaccagc gcgactgcgt gtcgagcgac gcgatgaact
     2821 gggccgcgtc gcgcggcatg atggtggccg tcgttttctc actgccagaa atgcgccccg
     2881 cggcgctcaa caacgcgccg gtcagcatga cgcggcggcc ggtggtgagt gccggcgcgc
     2941 cgagcccgct ccacggaccg gatgccttgt agcgcgccac gggatcgaag ccgcgttgca
     3001 cttcggggcg cgcgtcgccg caggtgagga aggtcttgtc cttgggcgac cagaacagca
     3061 tcgtgagacc gatgtagccc gaagccgaac gccacgcgag tgagcccaag cctgacaact
     3121 cgatctggcc ggcgtcttcg tattcgttgc gcgcccgccc gatcagtggt gcgggcgccg
     3181 atccgcgcgc cgcggccgac tcgatcgcgg cgaccagtgc gtaggcaatg gtgagctcgt
     3241 cgaacaggcg atgttcgtcg gcgccgccgg cgcgttcgag caacatctcc acgtgatcgg
     3301 cgagccgccg caacagcagt gcgaggcggt aacactgggc gccttgcgcc cacaccgcga
     3361 gcgtcgaaaa gcgttcctgg atgccgggcg agaaatgcga gaggccgagc gtgacgcact
     3421 cgcaggcgag ctggcggacc gcggcgcaaa gccgggtgcg cgactcctgc agcgaagccg
     3481 atgcggtttc ggcgcccgcg tggtccttgc cgagatcgag cttgccgtcc tggggagccg
     3541 gaccttcggg cgccgtcagc ggcacgccgt tggcacgctg atacgccatg accgcggccg
     3601 cgcggtactt ctcgagttgt ttgatgtcgg tgtcggccag catcccgtcg agcggcccgc
     3661 ccatgtagcg gaacaccagg cgcgggtggc ggaacgcgat caccacgttg cgctcgccgg
     3721 agagcgtgag gtccgtttcc acgttcagat cctggacgaa ttgccaggcc cagcgatatc
     3781 cggccttgcc ggcatgtttg gcgagttcgc gcggcgtcat cgcgagcagc gcggcgcgta
     3841 gctgatcgag cgccggcgcg gcgggtgcgt ccgcgggcgt cgccgccgcg ggcacatcgg
     3901 tggcggcggt gatgccgccc ttcggtgacg cggcctgcag cgtgatggcg gccgcgagta
     3961 tgtgctggca gacgccgctc gccgggcagc tgcactcggc gtgcgccgcg ccgcgcgcat
     4021 cgaatcgaat gcgatgctcg ccgaccttca gtaccagcaa tgcgggcgtt tcctcgacga
     4081 tctccgccgc gcccttttcc agatccttgc gcgcacgccg caggaggccg cggttgccga
     4141 gcgccgcgaa ggcatcgtcg tcgaagcgct gcaactgagc ctcgagtttt tcgcgcagcg
     4201 tgctcatgag atggccttca ccagccagtg ggcgagccgc tgcggcgtga gcgccgcgat
     4261 ctccatgccg caggccgcga gtttctccgc catctgccgg tcgtacaccg ggtgcgcctg
     4321 gccatcgagc gccgcgaggc ccagtagttt gacgcgcgcc tcggcgagcg cacgcaccgc
     4381 gcgcaccagc tcgcccgcgg gggcgccttc gcagaagtcc gtgaccagca cgagtatcgt
     4441 gcgctgcgga ttctcgatga tctgcgcgca gtagcgcatg gcctgcgcga tgtcggtgcc
     4501 gccgcccagt tgcaccttca tgaggatttc cacgggatcg tcgacgtgat cggacaggtc
     4561 gaccacctgg gtatcgaaca ccacgagctt cacgcgaaac gcgggcaggc ccgcgaggat
     4621 gccggccatg acggcgctgt ggatcaccga gtccgccatg ctgccgctct ggtcgatgca
     4681 caacacgatg tgccagggca ggcgccgcgc gttgcgctcg aagaaacgca gctcttcgat
     4741 gaccagcttg cggcgctcga cgtcgtagtg cttgagattg cggcgcaccg tgccgggcgc
     4801 gtcgaagttc tgcgccacgg ccatcggcga gtggcggaag cggttcagct ttccggcgag
     4861 cgtctggcgc acttccggtt cgagcttgcg acggatttcc tcgatcacct tgcggatgat
     4921 ctttcgcgcc acgtcgagca cttcaccttt gagatgcccg cgcagcgtga gcagcgtgcg
     4981 caacaattgc tggttgggtt cgaggcgttg cagcgttttc ggatcggtga cgagttcggt
     5041 cagcccgtaa cgatcgagcg cgtgtttctc gatgacctca accgtgtcct gcggaaacag
     5101 ctcgcgcact tcgttgagcc aggtgacgag ctggagctgc gacgggtcga gtgaacccgg
     5161 ggcgatttcc ttgcgcagac cgcgaccctc gtattcacgg ccataaaggt agtcgagcgc
     5221 ctggtcgcgg cgccggtcct ggccctgcat gccgccggtc ccgcccgaca tccggcgttc
     5281 ggcatatttg cccagcacca gccgccagcg tgtcaggtct ttcacggttt gccccacgcg
     5341 gcgagaccgt cgccttcgag cacctctaac aaggtgcccg acaacgcgag gttggcgcga
     5401 agctggccct cgccgacgtc atagttcatg agcgggccga tcgtcttttc tccgtgtagc
     5461 gcggccacgg cctcggcgac ccggtccgtc tccttcggcg tcatggccgc gaacgccagc
     5521 cgcagatccg gcagcaccgc gatgaactgg gcttcgtccc agccagagag cagcgtgtcg
     5581 agcgaggtca ggagctcggg ctgctgccag gccgcgtcgc gcgccgcatg cagcaggccg
     5641 cgcaggaatc ccacggagcg ctgcgcgtcg gtgatgccga tgaagtgacc ctgcatcgcg
     5701 ttcgcgagtt cggccgcgct gtaattgccg gccgagtaga ggagaccgac ggccgtaccg
     5761 cgaatcagcg gttcgtcgtg ggactccgcg agctgcctca ccatgtccca gtagaggctg
     5821 gcgtccagtt ccgcgccggc ctggctgacc aaaagttcgc gcagccgcgc gagggcatgc
     5881 atggtgtcgt tgccgcggct ggcactgccc gcgggcatgc ctcgcaggtt gctgcccagg
     5941 tagatagccc gctcgtaggc ggcgcgcagc accacgggaa gttccgcgac gtcgcgggct
     6001 tcgagcggct cacgcgattc ccacaacatg ccgaggctgc ccgtggccag cgccaccgaa
     6061 tcgaacgagg gatccgcgcc gatggcgccg cgcagcacgt cgagcacgcg cggcaggtga
     6121 tcgtgcaggc cgagcacgca gccacgcgcc agcagactag ccgccgcgcg cgcatcgcgc
     6181 gcttcggcgc cggccaccag gctgtcgagc agcgcgcgga accggtccgc cacggcgagg
     6241 ggcaccgtga cgccgtgcac cgcggcctcg accagcgagg cttcggtggc cgcggtgaac
     6301 gtgtattccc agtgctcctg cagtcggtcg aggcccgtgc c
//
LOCUS       DATDEU010000672         4652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1444815, whole genome shotgun sequence.
ACCESSION   DATDEU010000672 DATDEU010000000
VERSION     DATDEU010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444815"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VIV63_16075"
     CDS             complement(<1..562)
                     /locus_tag="VIV63_16075"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY6126171.1"
                     /translation="MSDSPNNLAFVFPGQGSQSVGMLASLAADSPVIRATFDEASGVL
                     GYDLWTLCSEGPLESLNETERTQPAMLVAGIATWRLWQQRGGREPAVVTGHSLGEFTA
                     LVAAGAIDFASCVDLVRYRGKVMQEAVPIGTGSMAALLGLEDADVEAACAQAAEGSVV
                     EAVNYNAPGQVVIAGEKAAVLRAIEAA"
     gene            complement(587..1201)
                     /gene="rrtA"
                     /locus_tag="VIV63_16080"
     CDS             complement(587..1201)
                     /gene="rrtA"
                     /locus_tag="VIV63_16080"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HEY6126172.1"
                     /translation="MNAKTPAHGLAGVLRSLNGDRAYGIALLCVCALLALPELFGEPA
                     RQALSFDRAALASGEWWRLLTAHFVHLDTEHAVLNGLGVVLMWALFARDYSPWRWAAI
                     YCGSALAVSAGLWFANPELEWYVGASGALHGVMTAGTIAHLRRRDLDGWILAAFIVVK
                     LGYEQFSGALPFAGSPDTIVDAHLYGAIGGVVLGLLLKSRREPL"
     gene            1254..1697
                     /gene="gspG"
                     /locus_tag="VIV63_16085"
     CDS             1254..1697
                     /gene="gspG"
                     /locus_tag="VIV63_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY6126173.1"
                     /translation="MTSRSLRAARGFTLIEIMVVVVIIGLLAAVILPNVFGNVERAQV
                     SKAKSDIQAIETALTMYKLDNYKYPSTDLGLTALSQRPNDPTVRNWREGGYIKRISND
                     PWGTPYQYVFPGTRGQEYDLYSFGADAQEGGEGPNADIGNWNLGQ"
     gene            1702..2277
                     /gene="gspH"
                     /locus_tag="VIV63_16090"
     CDS             1702..2277
                     /gene="gspH"
                     /locus_tag="VIV63_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HEY6126174.1"
                     /translation="MRATGARSAGFTLLELVVVVFIIGLITAAAVITFGGESRDTELD
                     REAERIDALLDYTREQAELQTRDYGLRIDALTYSFVVFDVMQNQWRTIDEDDALRERE
                     FPEGIRPAVVVEGRPIVLDVKKSQIQDFKPQILIFANGDLTSFEIFLQREGTAERDRA
                     RLYTDEQTNINLLLPGETEPQRAPARTALRK"
     gene            2274..2879
                     /gene="gspI"
                     /locus_tag="VIV63_16095"
     CDS             2274..2879
                     /gene="gspI"
                     /locus_tag="VIV63_16095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEY6126175.1"
                     /translation="MKHSTRARGFTLLEVLIALAIVAMSVGALLGTVTSSASNVIYLK
                     DKTLAEWVALNRLTEIRIDQNMPSEGKRSGNTVMGGMRWEWQEEVAELPVVKGLFRIE
                     VRARATGEFVDDTRATTKPTSQALSPSTSSGSSADKLNWTTSVIGVVGSARSDVRQDV
                     AAKIVGNPQVGNPQGNPQGNPQTGQPGGPPPTPPKTSTPAS"
     gene            2879..3508
                     /gene="gspJ"
                     /locus_tag="VIV63_16100"
     CDS             2879..3508
                     /gene="gspJ"
                     /locus_tag="VIV63_16100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HEY6126176.1"
                     /translation="MRTRGFTLIEVVIAMFIAAIMFAIGYSAINQALVDRDALNVSQE
                     RVTEIQRGMRVVAQDFAQVVARGARDTAGSGELMPAMLADGRSDAIVTFTRGGWSNPA
                     GVQRPAEQRVRYRFIDGALIREHWLSVDPALNAEPRQRTLLTKVTAVEIRFLDPVSRQ
                     WRTDWPTSGVTGQVNPVDVDRLITRPIAIEFTVVFEDWGRVQRLFEIPT"
     gene            3505..4467
                     /gene="gspK"
                     /locus_tag="VIV63_16105"
     CDS             3505..4467
                     /gene="gspK"
                     /locus_tag="VIV63_16105"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEY6126177.1"
                     /translation="MIHARQRGIALLVAIVMFAIATTVAAAITYNKAMAARRAAATFT
                     LEQALQAGMAAEALAGIALEDDAKNQKTTPDKGWAEPLGPVEIEGTGIWIQAQLEDLN
                     ARFNLNSLLMFQGPPDNLFVANPEQVQVFRKLLETLKIDPHYADLLVDWLDTDIAPQA
                     TQGGEDTLYLTQVPPYRPPNTFIWHASELLALPGFGAERYALIAPYVTALPADAKVNV
                     CTASGLVLDVVMNDPQNEQEFQLQDMDKLRASGCYPAKTVYANIISSPALKTAAESRV
                     EEKSSWFRLRTNIRVGTAEFVLYSVLFREANNRVRTVQRSFGSE"
     gene            4467..>4652
                     /locus_tag="VIV63_16110"
     CDS             4467..>4652
                     /locus_tag="VIV63_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126178.1"
                     /translation="MSDSLIIQLRDGAAPQWMVCGEDGRVVVEAVSGDLAQATAMATG
                     RRVVVILPASEALATESD"
BASE COUNT          910 a         1525 c         1451 g          766 t
ORIGIN      
        1 tggccgcttc gatggcgcgc agcaccgcgg ccttttcgcc ggcgatcacc acctgccctg
       61 gcgcgttgta gttgacggct tcgaccaccg agccttccgc cgcctgtgcg caggccgcct
      121 cgacgtcggc atcttccagg cctaacaagg cggccatgga gcccgtgccg atcggcaccg
      181 cctcctgcat gaccttgccg cgataacgca cgaggtcgac gcaactggcg aagtcgatcg
      241 cacccgcggc cacgagcgcg gtgaattcgc ccaggctgtg tcccgtgacg acggcgggtt
      301 ctcggccgcc gcgttgctgc catagccgcc acgtggcgat gcccgccacc agcatggcgg
      361 gctgggttcg ttcggtttca ttcagcgact cgaggggacc ctcgctgcac aacgtccaca
      421 ggtcgtaacc gaggacgccg gaggcttcgt cgaatgtggc gcgaatgacg ggggaatcgg
      481 cggcgaggct cgccagcatg ccgaccgact gggatccctg accgggaaat acgaaagcaa
      541 ggttgtttgg gctgtctgac atgggcgcgg agtataacgg ctccgttcaa aggggttccc
      601 ggcgcgattt cagaagaagt cccaggacta caccgccgat cgcgccatac agatgcgcat
      661 cgacgatagt gtcgggcgac cccgcgaacg gcagagcgcc cgagaactgt tcgtagccca
      721 gtttcaccac gatgaaggcc gcgagaatcc atccgtcgag atcacgacgg cgcagatggg
      781 cgatggtccc ggctgtcatc acgccgtgca gcgcaccgga agcgcccacg taccattcca
      841 gttccggatt ggcgaaccac aggccggcac tgaccgcgag cgcggagccg caatagatag
      901 ccgcccagcg ccacggggaa taatcgcgcg cgaacagcgc ccacatgagc accacgccca
      961 gcccgttgag gacggcatgt tcggtgtcca ggtggacgaa atgcgccgtc agcaggcgcc
     1021 accactcacc cgaggcgagc gccgcccggt cgaaactcaa cgcctggcgg gcgggttccc
     1081 cgaaaagctc gggcagcgcc agcagcgcgc agacgcacaa caacgcgatt ccgtaggcgc
     1141 ggtcgccatt gagcgaccgg agaacgccgg ctaacccatg ggcaggggtc ttggcgttca
     1201 tttgctacta tcgctcaccg atttctcacc gttttgggcc acctggggta ttcatgactt
     1261 ctcgttcgct tcgagccgca cgcggcttca ccctgatcga aatcatggtg gtcgtggtca
     1321 tcatcgggct gctggcggcc gtgatcctgc ccaatgtgtt cggaaacgtc gagcgagcgc
     1381 aggtgtccaa agccaagagc gacatccagg cgatcgaaac cgcgctgacc atgtacaagc
     1441 tcgacaacta caagtatccg agcaccgacc tcgggctgac cgcgttgtca cagcgtccca
     1501 acgatcccac cgtgcgcaac tggcgcgagg gtggctacat caagcgcatc agcaacgatc
     1561 cctggggcac gccgtatcag tacgtgttcc cgggcacgcg tggccaggag tacgacctct
     1621 attctttcgg agccgacgcc caggaaggcg gtgaaggtcc gaatgccgac attggcaact
     1681 ggaacctcgg gcagtaattc catgcgcgcc acgggcgcca gatccgcggg cttcacgctg
     1741 ctcgaactgg tggtcgtggt gttcatcatc ggcctgatca ccgcggccgc ggtcatcacc
     1801 ttcggtggag agagtcgtga caccgaactc gatcgggaag ccgaacgcat cgacgcgctg
     1861 ctcgactaca cgcgcgagca ggccgaactg cagacgcggg actacggctt gcgtatcgac
     1921 gccctcacct actcgttcgt ggtattcgac gtgatgcaga accagtggcg gaccatcgac
     1981 gaggacgacg cgcttcgtga acgtgagttt cccgaaggca tccggccggc ggtcgtggtc
     2041 gaaggcaggc ccatcgtgct cgacgtgaag aaatcgcaga tacaggactt caaaccgcag
     2101 atcctgatct tcgcgaacgg cgacctgacg tccttcgaaa tattcctgca gcgcgaagga
     2161 accgccgagc gcgaccgcgc gcgcctgtac accgatgagc agaccaacat caatctgctg
     2221 ctgcccggag aaaccgaacc gcagagagcc ccggcgcgta cggcgctgcg caaatgaaac
     2281 attcaacgcg cgctcgggga ttcacgctgc tcgaagtgct gattgcgctc gccatcgtcg
     2341 cgatgtcggt gggcgcgcta ctcggcaccg tgacctcatc ggccagcaac gtgatctatc
     2401 tgaaggacaa gacgctggcg gaatgggtgg cgctcaatcg cctgaccgaa atccgcatcg
     2461 accagaacat gccctcggaa ggcaaacgat ccggcaatac cgtcatgggc ggcatgcgat
     2521 gggagtggca ggaagaagtc gccgagctgc ccgtcgtcaa gggtttgttc cgcatcgaag
     2581 tgcgcgcgcg tgcgaccggc gaattcgtcg acgacacgcg ggcgaccacc aagccgactt
     2641 cgcaggcact ctcgccgagc acgtcgagcg gttcgtcggc cgacaagctc aactggacca
     2701 ccagcgtcat cggtgtcgtg ggcagcgcgc gctccgacgt gcgacaggac gtcgccgcga
     2761 aaatcgtcgg caatccgcag gtcggcaacc cgcaaggtaa tccgcagggc aatccgcaaa
     2821 ccggccagcc tggtggtccg ccgccaacgc ctcccaagac ttcgaccccg gccagctgat
     2881 gcgcacgcgc ggcttcaccc tgatcgaggt ggtcatcgcg atgttcatcg cggccatcat
     2941 gtttgcgatc ggctacagcg cgatcaacca ggcgttggtc gatcgggacg cgctcaacgt
     3001 ttcgcaggag cgggtcacgg aaatacagcg tggcatgcgc gtggtcgcgc aggacttcgc
     3061 gcaggtcgtg gcgcgcggcg cgcgcgacac ggccggatcc ggcgaactga tgcccgcgat
     3121 gctcgccgat ggtcgcagcg acgccatcgt cacgttcact cgcggcggct ggagcaaccc
     3181 ggcgggcgtt cagcgtcccg ccgaacaacg cgtgcgttac cgcttcatcg acggggcgct
     3241 gatccgcgag cactggctgt cggtcgatcc cgcgctcaat gccgaaccgc gtcagcgcac
     3301 gctgctcacc aaggtcacgg cggtggaaat ccgttttctc gatcccgtct cgcgtcaatg
     3361 gcgcacggac tggccgacat ctggtgtcac cggccaggtc aatccggttg acgtcgatcg
     3421 cctgatcacc cgcccgatcg ccatcgagtt cacggtggtg ttcgaagact ggggccgcgt
     3481 gcaacgcctg ttcgagatcc ccacgtgata cacgcgcgtc aacgcggcat cgcgctcctg
     3541 gttgccatcg tgatgttcgc gatcgccacc accgtcgccg cggcgatcac ctacaacaag
     3601 gcgatggcgg cgcgacgcgc ggccgcgacc ttcacgctgg agcaggcact gcaggccggc
     3661 atggccgccg aagcgctcgc gggcattgcg ctcgaagacg atgccaagaa tcagaagacc
     3721 acgcccgaca agggctgggc cgagccgctc gggcccgtgg aaatcgaagg aaccggcatc
     3781 tggatccagg cgcagctcga agatctcaac gcgcgtttca atctcaacag cctgctgatg
     3841 ttccagggtc cgccggacaa cctgttcgtc gccaatcccg agcaggtcca ggttttccgc
     3901 aaactgctcg agacgctgaa gatcgatcct cactacgccg acctgctggt agattggctc
     3961 gacaccgaca tcgcgccgca ggcgacgcaa ggtggcgagg acaccctgta cctcacccag
     4021 gtaccgccgt accggccgcc gaacaccttc atctggcatg catccgagct gctcgcgttg
     4081 ccgggattcg gcgccgaacg gtacgcgctg attgcgccct acgtcaccgc cctgccggcc
     4141 gacgccaagg tgaacgtctg cacggccagc ggcctggtgc tcgacgtcgt catgaacgac
     4201 ccgcagaacg aacaggaatt ccagctccag gacatggata aactgcgcgc ctccggctgt
     4261 tatcccgcga aaaccgtcta cgccaacata atcagcagtc ccgcgctcaa gaccgcggcc
     4321 gaaagtcggg tcgaggaaaa atcttcatgg ttcaggttgc gcaccaacat tcgggttggc
     4381 actgcggagt tcgttttgta cagtgttcta ttccgcgagg ccaacaaccg agtacgtacc
     4441 gtgcaacgca gttttggaag tgagtgatga gtgattcact gatcattcaa cttcgcgatg
     4501 gcgcggctcc ccagtggatg gtgtgcggcg aagacggccg agtcgtggtc gaagcggttt
     4561 ccggcgacct cgcgcaggcc acggccatgg ccacgggccg cagggtcgtg gtgatacttc
     4621 ccgccagcga agcgctggcc accgaaagcg at
//
LOCUS       DATDEU010000673         3969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1153863, whole genome shotgun sequence.
ACCESSION   DATDEU010000673 DATDEU010000000
VERSION     DATDEU010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153863"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..2724)
                     /locus_tag="VIV63_16115"
     CDS             complement(205..2724)
                     /locus_tag="VIV63_16115"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY6126179.1"
                     /translation="MSASGFAGLGLQIVWTQQSALWLGHETAAVLAVIAAFFGGLAVG
                     ALALGSRIDRSERPARWYAGCEAVIGLWSFALLFLMAPVSDGLLGFIGAQPAPLTQWT
                     VVFCGTFALLLPATAAMGATLPAMERVIGTAGNDRTPIAMLYAANTFGAVLGVVAAAF
                     VLVPRFGLILTAAFCLATNFFCAALSLKVFAKRVPATPAIPASVQSHLPILAITGLLG
                     IGYEVLVVRVLSQVAENTVYTFAILLAMYLVGTTLGAAAYARWSGAMAPERMRDRLLQ
                     TLAAACLVGVALLAFAGEMKSVLLSLLGPGMAAALFAESVLAIAAFLLPTFVMGALFS
                     HLAGSARASGVSFARTIGFNTLGAAFAPVLFGVALLPALGSTFALLAVAGGYLLLSTR
                     AAWFKPAQWSAVAGVVAFVVWAPSLALIELPVGGRVISYTEGAAATVSVIENEDGVAT
                     LHINNRQQEGSSVTLLADARQGLLPILLHPSPRRALFLGVGTGVTSRSAAEDSSLEVD
                     AVELLPEVVAASGFFTRAFGEQMNPRLRVLTADARRFVRTAPEFYDVIVADNFHPARS
                     GSGSLYTVEHFAAVRERLTAGGLFCQWLPLHQLDLDTLRSIVGSYLEVYPRGTAILAT
                     NSLETPVLGLVARRNGERLDLDAVRARLAGAKFSRPLADFGLADDFALLGSFVASAGS
                     LARFAAGAPLNTDDHPVVTYLAPRITYAPDSAPRDRLLEFLGQVESDSTELFETRDAN
                     HEARLAAYWQARDRFLEVGRNVQPTQDVQQMLAQVRDPLLSVLHISPDFRPAYDPLLR
                     MAYGMATRDSGAARELLALLVREQPARPEAAEALRALGASP"
     gene            complement(2754..3377)
                     /locus_tag="VIV63_16120"
     CDS             complement(2754..3377)
                     /locus_tag="VIV63_16120"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY6126180.1"
                     /translation="MQEVIGLFPTPFMRATRVLPGDLVAGLIEHFSQSVHQANSSSPN
                     LVHTALLRPSDSPLLVDVAALLSPLLPEYGALLFGERLGWSLKEMWVNVLDTGGRQAM
                     HNHANSFISGVVYLTHTHAESRTVFMKSPGGTDFAFKNDHAGVTTGAYNADKWISPLP
                     EPGDVVLFPSYLMHAVPLNPGERRISLAFNAIPTHLDSWGYRVSFSG"
     gene            3542..>3969
                     /locus_tag="VIV63_16125"
     CDS             3542..>3969
                     /locus_tag="VIV63_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126181.1"
                     /translation="MKIEFKNTQIAIAVAGALSAAAMTAAYAGAPVAQPKTTGTYVTG
                     DFHNHTTCSDGSISMQKLIKKSTDTTDTPWGLDWFVQAGHGGNGNRNCTLAEDATLAT
                     PAYPLVLATDGTTIQGPNTSWQNTNPAINPKGRVQGSAPN"
BASE COUNT          840 a         1322 c         1214 g          593 t
ORIGIN      
        1 tcgccgacac gcctcgtgtg aggtccggtc ctttcagttc gctcgtgccg cccatgtatt
       61 gctccgggat gtgcaggtcg cgcctgggtt tgcgatcata agtcagcgca acgcacggaa
      121 tgagtacttc gggaacgaca cgatcccgac atcgaactca gtatttacgt ttcaccagat
      181 ggaaagctga cgagagcctg ggacttaagg tgaagccccc agtgcgcgta gcgcctccgc
      241 agcttcaggt cgggcgggct gctcacgcac gagcaatgcc agcagttctc tggcagcacc
      301 ggaatcccgc gtcgccattc cgtacgccat gcgcagcagc gggtcatacg ccggacggaa
      361 atccgggctg atgtgcagca ccgacagcag cggatcacgc acctgcgcca gcatttgctg
      421 tacatcctgc gtcggttgca cattgcgacc cacctcgagg aagcgatcgc gcgcctgcca
      481 gtaggcggcg agtcgcgctt cgtggttcgc atcgcgcgtc tcgaacaatt cggtggaatc
      541 gctctccact tgcccgagaa actccaaaag ccgatcacgc ggcgccgaat ccggcgcata
      601 ggtgatgcgc ggtgcgagat acgtcaccac gggatgatca tcggtattga gaggcgcacc
      661 ggccgcgaac cgcgccagtg agccagcact cgccacgaag ctccctagca acgcgaaatc
      721 gtccgccaat ccaaaatccg cgagcggccg cgaaaacttc gcgcccgcga gacgcgctcg
      781 cacggcgtcc agatccagcc gttcaccatt ccgccgcgcg accagcccga gcaccggagt
      841 ttcgaggctg ttcgtggcga gaatcgcggt gcctcggggg tacacctcga gataggaacc
      901 cacgatgctg cgcagcgtgt cgaggtcgag ctgatgcagc ggcagccact ggcagaacag
      961 accgccggcc gtgagacgct cgcgcaccgc cgcgaaatgc tccacggtgt acagagaacc
     1021 ggatccgctg cgcgcgggat gaaaattatc agccacgatc acgtcgtaga actcgggcgc
     1081 tgtcctgaca aaccggcgcg catccgcagt cagcacccgc agccgcggat tcatctgctc
     1141 gccgaaagcc ctggtgaaga agccggacgc ggcgacgacc tccggcaata actccacggc
     1201 gtcgacctcc agcgacgaat cctccgcggc cgaacgcgac gtcacgcccg tgcccacgcc
     1261 gagaaacagc gcgcgacgtg gcgaaggatg cagcaggatc ggcaacaagc cctggcgcgc
     1321 atcggccagc agcgtgacgc tgctgccttc ctgctggcga ttgttgatgt gtaacgtagc
     1381 tacgccgtct tcgttctcga tcacgctgac cgtggcggcc gcgccttcgg tgtagctgat
     1441 cacgcggccg cccaccggta gttcgatgag cgcgagcgac ggcgcccaca cgacgaacgc
     1501 caccacgcca gcgactgccg accattgcgc gggcttgaac caggctgcgc gcgtcgacag
     1561 caacaggtat ccgccggcga ccgcgagcaa cgcaaaagtc gatccaagcg cgggcaacag
     1621 cgcgacacca aacaagaccg gtgcgaatgc ggcgcccagc gtgttgaacc cgattgttcg
     1681 cgcgaaactc acgcccgacg cgcgggcact accggcaagg tggctgaaga gagcgcccat
     1741 cacgaacgtg ggaagcagga acgccgctat tgcaagaacg ctttcggcaa acagtgcggc
     1801 tgccatgccg ggccccagca ggctcagaag cacgctcttc atctcaccgg cgaacgcgag
     1861 caatgcgacg ccgacgagac acgccgcggc caaagtctga agcagccggt cgcgcatccg
     1921 ttcgggcgcc atcgcgccag accatcgtgc gtacgcagcg gcgcccagcg tcgttccgac
     1981 gaggtacatc gcgagcagga tcgcaaacgt gtacaccgtg ttctcggcca cctggctcag
     2041 caccctgacc accagcactt cgtagccgat gccgagtagc ccggtaatcg cgagaatcgg
     2101 taaatgtgac tgcaccgagg cgggaatggc cggcgtcgcg ggcactcgct tcgcaaacac
     2161 cttcaacgaa agcgctgcgc agaagaagtt ggtcgcgagg cagaacgccg cggtcaagat
     2221 caaaccgaaa cgcggcacca gcacgaacgc cgccgcaacg actccaagca cggcgccaaa
     2281 ggtattggcg gcatacagca tggcaatcgg cgtgcgatcg ttgcccgccg ttccgatgac
     2341 ccgctccatg gcgggcaacg tcgcgcccat cgccgcggtg gccggaagca gcaacgcaaa
     2401 tgtgccgcaa aacacgacgg tccattgcgt gagcggagcc ggctgcgcac cgatgaaccc
     2461 caacaacccg tcggacaccg gcgccatcag aaacagcagc gcgaaactcc acagaccgat
     2521 gacggcttcg caccccgcgt accagcgcgc cggccgctca ctcctgtcta tgcgtgaccc
     2581 gagcgccaat gcaccgaccg cgagtccacc gaaaaacgcc gcgatgaccg cgagcacggc
     2641 cgcggtctcg tgcccgagcc acaatgcact ctgctgagtc cacacgatct gcagcccgag
     2701 cccggcgaag cccgatgcgg acatcagcac gaggctccag gatcgcaacg ggctcaaccg
     2761 ctgaaagaaa cgcgataacc ccaggaatcg aggtgcgtcg gaatggcgtt gaaagccagc
     2821 gatatccgcc gttcaccggg gttgagcggc acggcgtgca tgaggtagct agggaacagc
     2881 accacgtcgc ccggttcggg cagcgggctg atccacttgt cggcgttgta ggcgccggtg
     2941 gtgacgccgg cgtggtcgtt cttgaacgcg aagtcggtgc cgccgggcga cttcatgaac
     3001 accgtgcgcg attccgcatg ggtatgggtg agatacacca cgccggagat gaagctgttc
     3061 gcgtggttgt gcatggcctg gcgtccgccg gtgtcgagca cgttcaccca catctctttc
     3121 agcgaccatc cgagccgctc gccgaacagc agcgcgccgt attccggcag cagcggactc
     3181 aggagtgccg cgacgtcgac cagcaaaggt gagtcgctcg gacgcagcag cgccgtgtgc
     3241 acgagattcg gtgacgagct gttcgcctga tgaacgctct ggctgaagtg ttcgatcaat
     3301 ccggcaacca gatcgccggg aaggacgcgc gtcgcccgca tgaatggcgt gggaaagagc
     3361 ccaatgactt cctgcatcga agttgttagc cggcgggagg gggctcagat tctagtgaca
     3421 gttgtgcgac agatttatga cggcgcgggt cttcatcgcg tcgcgtgggc ttaatggcac
     3481 ggtcatatgc agcgcctagg gttctccccc tgaatcgacc gacctcatgg ggaatccttc
     3541 aatgaaaata gagttcaaga acacgcagat cgcaatcgca gtggccgggg cgctcagcgc
     3601 cgcggcaatg accgccgcat acgcaggagc gcccgtcgct caacccaaga ccacaggcac
     3661 gtacgtcacg ggtgacttcc acaaccacac gacgtgctcc gacggctcga tctcgatgca
     3721 gaagctcatc aagaaatcga ccgacaccac cgacactccc tggggtctcg actggttcgt
     3781 gcaggccggt cacggcggca acggcaatcg caattgcacg ctcgccgaag acgccacgct
     3841 tgcgactccc gcttatccgc tcgtgctggc caccgatggc accaccatcc agggtccgaa
     3901 caccagctgg cagaacacga atcccgccat caacccgaag ggccgcgtgc agggcagcgc
     3961 ccctaacca
//
LOCUS       DATDEU010000674         2398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_715347, whole genome shotgun sequence.
ACCESSION   DATDEU010000674 DATDEU010000000
VERSION     DATDEU010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715347"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /gene="pgi"
                     /locus_tag="VIV63_16130"
     CDS             complement(<1..1266)
                     /gene="pgi"
                     /locus_tag="VIV63_16130"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY6126182.1"
                     /translation="MTHDPMTAAWRALQARATETRAVSLRALRDADPARGTRFAREAA
                     GLYLDFSRQRVDDDGLHLLSSLADAAGLRARIEAMWRGEPINLTEGRAVLHTALRVPG
                     VSASGPGGEEIARQVLAERERMLEFAENVREGVVRGSGGARFTTVINIGIGGSDLGPA
                     MAVQALHPLTAHAPRVLFVSNVDGTDLANALEGTDASRTLFIVASKTFSTQETLANAR
                     SARQWLVGELGEAAVPAHFAAVSTNAQAMDAFGINPNYRFMMWDWVGGRYSLWSSIGV
                     SLAIAIGRGAFLELLAGAHEIDEHFRSAPWERNLPVLMGLLGVWNINFLELPTLAVLP
                     YDGRLGRFSAYLQQLDMESNGKSVNLAGQPLSHSTAPVIWGEPGNNAQHSFFQMLHQG
                     TPRAALDLLVPAQSSCGNPAQAELAIGNCL"
     gene            complement(1337..>2398)
                     /gene="rfbB"
                     /locus_tag="VIV63_16135"
     CDS             complement(1337..>2398)
                     /gene="rfbB"
                     /locus_tag="VIV63_16135"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY6126183.1"
                     /translation="AGFIGSNFVHYWRKAHPEDRIVVLDALTYAGNFHNLAGLEASSF
                     RFVHGDIASQELVTSLLRAEKIDTLVHFAAESHVDRSITGPDVFIATNIVGTHSLLKA
                     AREVWLEERAVPEHRFHHVSTDEVFGSLGPQDPPFSETTPYAPKSPYSASKAASDHLV
                     RAYHHTYGLRVTTSHCSNNYGPRQFPEKLIPLCIVNMLLGRPLPVYGDGRHVRDWLHV
                     DDHCRGIDLILGQGRAGEVYNLGAGAERANIDLVTSLCQHVDDAFRRSPELARRFPAA
                     PGARGASAQSLIIHVSDRPGHDRRYGLDTRKARRELGFEAGRALAQGLEDTVGWYLAN
                     ESWWDAILTGRYRHATLPN"
BASE COUNT          410 a          752 c          857 g          379 t
ORIGIN      
        1 gaggcaattt ccgatggcga gttccgcctg ggcgggattg ccacacgagg actgcgccgg
       61 caccagcagg tcgagggccg cgcgcggcgt gccctggtgc agcatctgga agaaggaatg
      121 ctgcgcgttg tttcccggct cgccccagat gaccggcgcg gtggaatggc tgagcggctg
      181 gccggcgagg ttcaccgact tgccgttgct ctccatgtcg agctgctgca ggtacgcgga
      241 gaatcttccg aggcggccat cgtaaggcag caccgcgagt gtcggcagtt ccaggaaatt
      301 gatgttccac acgcctaaca ggcccatgag gaccggcagg ttgcgctccc acggcgcgct
      361 gcggaaatgt tcgtcgattt cgtgcgcacc ggcgagcagt tcgaggaagg cgccgcgtcc
      421 gatggcgatg gccagcgaca cgccgatcga cgaccacagc gaataacggc cgccgaccca
      481 gtcccacatc atgaaccggt agttaggatt gatgccgaac gcatccatcg cctgcgcgtt
      541 ggtggagacc gcggcgaagt gggccggcac cgcggcttcg ccgagctcgc cgaccagcca
      601 ctgccgggcg gagcgtgcat tggcgagtgt ttcctgcgtg ctgaacgtct tcgaggcgac
      661 gatgaacagc gtgcgcgacg cgtcggtgcc ttcgagcgca ttggcgaggt cggtgccgtc
      721 gacgttggaa acgaacagga cacgcggcgc atgcgcggtg agcggatgca acgcctggac
      781 cgccatcgcg ggcccgagat ccgagccgcc gatgccgatg ttgatgacgg tggtgaaacg
      841 ggcgccaccg gaaccgcgca ccacgccttc gcgcacgttc tcggcgaatt ccagcatgcg
      901 ttcgcgttcg gcgagcacct gtcgcgcgat ttcctcgccg ccggggccgc tggccgaaac
      961 gccgggcacg cgaagtgcag tgtgaaggac ggcgcggcct tcggtgagat tgatgggctc
     1021 gccgcgccac atggcctcga tgcgggcacg caacccggcg gcatcggcga ggctcgacag
     1081 cagatggagg ccgtcgtcgt cgacacgctg ccgcgagaaa tcgagataga ggccggcggc
     1141 ctcacgcgcg aaacgagtgc cgcgcgccgg atcggcgtcg cgcagcgcac gcaaactcac
     1201 ggcacgggtt tccgtggcgc gcgcctgcag agcgcgccag gcagcggtca tggggtcatg
     1261 ggtcatcggg cggcgatggt atcgacaacc cgttgaattt gcgactccaa acgcgttccg
     1321 cggcggcggc gcctgctcag ttcgggagcg tcgcgtggcg gtaccggccg gtcaggatcg
     1381 cgtcccacca ggactcgttc gcgaggtacc agccgaccgt gtcctcgagt ccctgcgcaa
     1441 gggcgcgccc ggcctcgaat ccgagctcgc gccgtgcctt acgggtatcc agaccgtagc
     1501 ggcggtcgtg tccgggccgg tcggaaacgt ggatgatcaa ggattgagcg gaggcgccgc
     1561 gcgcaccggg cgccgcgggg aaacgccgtg ccagttccgg cgagcggcga aaggcgtcgt
     1621 cgacgtgttg gcacagggag gtcacgagat cgatgttggc gcgctccgcg ccggcaccca
     1681 ggttgtaaac ctcgcccgcg cggccctgcc cgaggatcag atcgatgccg cggcagtggt
     1741 cgtcgacatg cagccagtcg cgaacgtggc ggccgtcgcc gtacaccggc agcgggcgtc
     1801 cgagcagcat gttcacgata cacaacggga tcagcttttc cgggaactgc cggggcccgt
     1861 agttgttgga acaatggctc gtggtgacgc gcaagccgta ggtgtggtga taggcgcgca
     1921 ccaggtgatc ggacgcggcc ttgctcgccg aatacggcga cttcggcgca tagggcgtgg
     1981 tctcgctgaa cggcggatcc tgcggcccga gtgagccgaa gacttcgtcg gtggacacgt
     2041 ggtgaaaccg gtgctcgggc accgcgcgct cttcgagcca gacttcgcgc gccgccttca
     2101 gcagcgaatg cgtgcccacg atgttggtgg cgatgaacac atcgggaccg gtgatcgagc
     2161 gatcgacatg cgattccgcg gcgaagtgca ccagcgtgtc tatcttctcg gcgcgcaaca
     2221 gcgaggtgac cagttcctgg ctggcgatgt cgccgtgcac gaagcggaac gacgaggcct
     2281 cgagcccggc gaggttgtgg aaattgccgg cgtaggtcag cgcgtcgagc accacgatcc
     2341 ggtcttccgg atgcgccttc cgccagtagt ggacgaaatt cgagccgatg aatccggc
//
LOCUS       DATDEU010000675         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1735865, whole genome shotgun sequence.
ACCESSION   DATDEU010000675 DATDEU010000000
VERSION     DATDEU010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735865"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /gene="flhA"
                     /locus_tag="VIV63_16140"
     CDS             complement(<1..1741)
                     /gene="flhA"
                     /locus_tag="VIV63_16140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY6126184.1"
                     /translation="MAEPDNNSSTSALMRMGVGVPIGLISILAMMILPLPPVMLDVLF
                     TFNIALSLVIVMAVFNVKRPLEFAIFPTVLLLVTILRLALNVASTRVVLMHGHEGSHA
                     AGKVIAAFGEFVIGGNFAVGAVVFIVLTIINFVVVTKGAGRVSEVSARFALDAMPGKQ
                     MAIDADLNAGIISQDEARVRRLEVRAESDFYGSMDGASKFVRGDAVAGILIMVINIVG
                     GLLIGTMMHDLPMGEAAKTYTLLTIGDGLVAQIPGLMLSVAVAIIVTRVTREQDMGSE
                     LQKQLFGQPKALAVAAAILGIMGVIPGMPNVAFLSIGALCGFAAWRLVKKREAEALLP
                     QAEPEPPPPPPSQENQELTWEDVRPVDAVGLEVGYRLVPLVDRTKGGDLLARIRGIRR
                     KLTQELGFLVQAVHIRDNLELGPNTYRIRLAGVAIGESVVHPDRELAINPGRVFGPVN
                     GMVTRDPAFGMEAVWIEPNLREHAQSLGYTVVDAATVIATHLSHLIQSHAHELFGHEE
                     AEQLLKNLAKTAPKLVDELVPKVLPLAVVVRVMQGLLAERVPIRNVRAIVEALAEAGP
                     RSQDPAVLQAQVRV"
     gene            complement(1751..>2215)
                     /locus_tag="VIV63_16145"
     CDS             complement(1751..>2215)
                     /locus_tag="VIV63_16145"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY6126185.1"
                     /translation="WQHSRDLKMTREEVKEEYKESEGSPETRGRIREAQRALARGRMM
                     QEVPKADVVITNPTHFAVALRYDETRNRAPIVVAKGTDLLAARIREIAAENGVPLVAA
                     PPLARALYRTVELGHEVPAALYVTVAQVLTYVYQLKAAMARGAKPPPPPVIN"
BASE COUNT          369 a          712 c          749 g          385 t
ORIGIN      
        1 caaccctgac ctgcgcctgc aatacggccg gatcctgcga acgcggaccc gcctccgcca
       61 aagcttcgac gatggcgcgc acgttgcgga tgggcacgcg ctctgcgagt aacccctgca
      121 tcacgcgtac gacgacggcg agcggcaata ctttcggcac gagttcgtcg acgagcttcg
      181 gcgccgtctt ggcaaggttc ttgaggagct gctcggcttc ttcgtggccg aacaattcat
      241 gcgcatgcga ctggatgaga tgcgacagat gtgtggcgat cacggtcgcg gcatcgacca
      301 ccgtgtagcc gagcgactgc gcgtgttcgc gcaggttggg ctcgatccac accgcttcca
      361 tgccgaacgc cggatcgcgc gtcaccatgc cgttgacggg gccgaacacg cggccggggt
      421 tgatggcgag ctcgcgatcg ggatgcacga cgctttcgcc gatggccacg cccgcgagcc
      481 gtatgcgata ggtgttcggg cccagctcga ggttgtcgcg gatgtgcacg gcctgcacga
      541 ggaatccgag ttcctgcgtg agcttgcggc ggatgccgcg gatgcgcgcc agcagatcgc
      601 cacccttggt gcggtcgacc agcggcacga ggcggtaacc gacttcgagt ccgacggcgt
      661 ccaccgggcg cacgtcttcc caggtcagtt cctgattttc ctgtgacggc ggcggtggcg
      721 gcggttccgg ctccgcctgc ggcagaagcg cttccgcttc gcgcttcttg acgagacgcc
      781 acgcggcgaa accgcagagc gcaccgatcg acaggaacgc gacgttcggc attccgggaa
      841 tgacacccat gatgccgagg atcgctgccg ccaccgcgag cgccttcggc tggccgaaga
      901 gctgcttctg caattcgctg cccatgtcct gctcgcgcgt gacgcgcgtg acgatgatgg
      961 cgaccgcgac cgacaacatg aggccgggga tctgcgccac caggccgtcg ccgatggtga
     1021 gcagcgtgta ggtcttggcg gcctcgccca tcggcaggtc gtgcatcatc gtgccgatca
     1081 gcagtccgcc gacgatgttg atgaccatga tgaggatgcc ggcgacggcg tcgccgcgga
     1141 cgaacttgct ggcgccgtcc atggaaccgt agaagtcgga ttcggcgcgc acttcgaggc
     1201 ggcgcacccg ggcttcgtcc tgcgagatga tgccggcgtt gaggtccgcg tcgatggcca
     1261 tctgcttgcc gggcatggcg tccaacgcga atcgtgccga cacttccgag acgcgacccg
     1321 cgcccttggt caccaccacg aagttgatga tggtgagcac gatgaacacc accgcgccca
     1381 cggcgaaatt accgccgatc acgaattcac cgaaagccgc gatgaccttg ccggctgcgt
     1441 gtgaaccttc gtggccgtgc atgagcacca cgcgcgtcga agcgacgttg agcgccaggc
     1501 gcaggatggt cacgagcagc agcacggtcg ggaagatcgc gaactcgagc ggacgcttga
     1561 cgttgaacac cgccatgacg atgaccagcg acagcgcgat gttgaacgtg aacagcacgt
     1621 cgagcatcac cggcggcagt ggcaggatca tcatcgcgag aatcgagatg aggccgatgg
     1681 gcacgcccac gcccatgcgc atcagcgcgc tcgtcgaaga attgttgtct ggttcagcca
     1741 ttgggttcta tcagttgatg acgggcggcg gcggcggctt ggcgccgcgc gccatcgcgg
     1801 ccttgagctg gtagacgtag gtgagcacct gcgccacggt cacgtagagc gcagccggca
     1861 cttcgtggcc gagctcgacg gtgcggtaca gcgcgcgcgc caggggcggc gcggcgacga
     1921 gcggcacgcc gttttcggcg gcgatctcgc ggatgcgtgc cgcgagcagg tccgtgccct
     1981 tggcaacgac aatgggtgcg cgattgcgcg tctcgtcgta gcgcagcgcc acggcgaagt
     2041 gcgtggggtt cgtgatcacc acgtcggcct tcggcacttc ctgcatcatg cggccgcgcg
     2101 ccagcgcgcg ttgcgcctcg cggatgcggc cgcgggtttc gggtgagcct tcgctctcct
     2161 tgtattcttc cttcacttcc tcgcgcgtca tcttgagatc gcgcgagtgc tgcca
//
LOCUS       DATDEU010000676         4793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1445468, whole genome shotgun sequence.
ACCESSION   DATDEU010000676 DATDEU010000000
VERSION     DATDEU010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445468"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..973
                     /locus_tag="VIV63_16150"
     CDS             101..973
                     /locus_tag="VIV63_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6126186.1"
                     /translation="MNIKDLVAIDVHTHAEVSCRHEPDEEWKKFEDASAKYFKAGAKR
                     PTIPETIAYYRERKIGLVMFTVDSEFEIGARRIPNEEVADAARENSDIMMAFASIDPH
                     KGKMGLREARRLINDGVIRGFKFHPTLQGFFPNDRIAYPLYELIAEHKLPAIFHSGHS
                     GMGTGMPGGGGLRLKYSNPIHLDDVAADFPDMTVIIAHPSWPWQDEALSVCLHKPNVY
                     IDLSGWSPKYFPPQLVKYANTQLKDRMLFGSDYPLITPDRWIKDFHEAGFRPEVHDLI
                     LKQNAIRALGLERA"
     gene            975..2819
                     /locus_tag="VIV63_16155"
     CDS             975..2819
                     /locus_tag="VIV63_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEY6126187.1"
                     /translation="MTAAAASWTPDPFATQFERRGDGSLILRPLGELSPYPPTLVNYL
                     EHWARTAPDRVLVARRDGAGAWRRVSYAQMLARIQRLAAGLLTMDLSRDRPIAILSGN
                     SIEHLTLSLAAMWVGIPYCPVSPAYSQVSTDLAKLQYVLDLLTPGLVVAFDTPRFARA
                     LSLVPAHAKIIGDALLDGRAVIALESLEQAVTAEVDAAHAATDADSIVRFLLTSGSTG
                     QPKAVITTNRMCCSNAAMLRQSMPFIAGEPPVLLDWLPWNHTFGGSHNIGMVLANGGS
                     LYIDDGRPTPAGISQTLQNLREISPTVYFNVPKGFEMLAPHLRSDAQLRKTFYSRLRA
                     YFFAGASLAQHTWDELDAASTLELGFKVPMLSGLGATETGPSVTFTTPAMGRSGVIGL
                     PAAGTLVKLAPVEEKLEIRVRSPSVTTGYWRQPELNAQAFDDEGFFRLGDAVRLIDPD
                     EPSRGLKFDGRIGEDFKLANGTWVSVGPLRAELISALAPVAQDVVIAGLDTDFVAILV
                     IPDIDACASMLACPHPPTRAELAGEPKLLSGIQQRLAAHARANPASSRGVRRACVLAV
                     APSLDRGEITDKGSINQRAMLRNHADLVAELYSPAPPPRIVIIEMERP"
     gene            2816..4024
                     /gene="mhpT"
                     /locus_tag="VIV63_16160"
     CDS             2816..4024
                     /gene="mhpT"
                     /locus_tag="VIV63_16160"
                     /inference="COORDINATES: protein motif:HMM:NF008586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HEY6126188.1"
                     /translation="MSAREETAVTVGLCFLVAVLEGFDIQAMGVAAPRLAPQFGLDAG
                     QMGWVFSISNFGLVIGASLGGWLADRSGRKPVLMGAVAIFGCFTLATAFAGDYHALLA
                     VRFLAGLGFGAALPNMMAIAAEISRPEKAASTAAIMFCGMPVGGGLAALTTQILPADI
                     DWRALFYVGGVLPVLLLPALRVWMPETLVRGTTLQSRDASVLGTLFGEGRATPTLLLW
                     VAFLPTLLILYLFLNWLPTLVIAKGLERAVAPQASLAFNFGSVAGALVFGALVDRFHF
                     RWPLTLAYLLLIVALLLLGASAGHAAIIALSGAVGFCLLGANYALYGVAPAYYPRAIR
                     GTGSGASIAVGRIGSIAGPLLAGLLIGGGTSAAGVVQYMVPVAAVSALAVFALSFRSR
                     ASETGRTSGS"
     gene            complement(3975..>4793)
                     /locus_tag="VIV63_16165"
     CDS             complement(3975..>4793)
                     /locus_tag="VIV63_16165"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6126189.1"
                     /translation="SVSSALGKLRTFFTDPLFVRSGNTMQPTPRAVEIMGAVREVLSR
                     IGTDIVAAPSFEPGRSHRPIVLALSDVGEVVFLPSILRQLRLEAPGVSLRSVSLPAED
                     VASGLESGTIDLALGYFPNLKRHNFFQQTLFSDSFTCLIRADHPVKSARLTQKQFRQL
                     DHAVVRAESRTEEVIERFLARHRIQRRVTLTTPHFASVPPIVEQSDLVVTVPVPLARY
                     FSTVSAGIRIIRPPFEAPSIELKQIWHRKFHHDARSRWLRTLMCGLFQKPAIES"
BASE COUNT          816 a         1521 c         1558 g          898 t
ORIGIN      
        1 ccatgcactg ggagccggcg ccgttgctag tggagctcgt cgagaagaaa ctgaccatcg
       61 cggagtggga gcgtcagcaa cgagcgaagg gggcgtagcc atgaacatca aggacctggt
      121 cgccatcgac gttcacacgc atgccgaggt gtcgtgccgt cacgagcccg acgaagagtg
      181 gaagaagttc gaggatgcgt ccgcgaaata tttcaaggcc ggagccaagc ggccgacgat
      241 ccccgaaact attgcctatt accgcgagcg caagatcggc ctcgtgatgt tcacggtcga
      301 ctcggaattc gagatcggcg cgcgccggat tcccaacgag gaagtggcgg acgcggcacg
      361 cgagaactcc gacatcatga tggcgttcgc gagcatcgat ccgcacaagg gaaagatggg
      421 tctgcgcgaa gcgcggcgcc tgatcaacga cggcgtcatc cgcggcttca agttccaccc
      481 gacgctgcag ggctttttcc ccaacgaccg cattgcctat ccactctacg agttgatcgc
      541 ggagcacaag ctcccggcga tcttccacag cgggcactcg ggaatgggta ccggcatgcc
      601 gggcggcggc ggcctgcgtc tcaaatattc caacccgatc cacctcgatg acgtcgcggc
      661 ggattttccc gacatgacgg tcatcatcgc gcatccatcc tggccctggc aggacgaggc
      721 gctctcggtg tgcctgcaca agccgaacgt ctacatcgat ctgtccggct ggtcgccgaa
      781 atactttccg ccgcagctcg tgaagtacgc gaacacccag ctcaaggacc ggatgctgtt
      841 cggctccgac tatccactga tcacgcccga ccgttggatc aaggatttcc acgaagccgg
      901 tttccgcccc gaagtgcacg acctgatcct caagcagaat gcgatccggg cgctcgggct
      961 tgagcgcgcg tgacatgacg gcggctgcgg cgagttggac accggatcca ttcgcgacgc
     1021 agttcgaacg ccgtggtgac ggctcgctca tccttcggcc actcggcgaa ctctcgccgt
     1081 atccgcccac actcgtgaat tacctcgagc actgggcgcg caccgctccg gatcgcgtgc
     1141 tggtggcgcg gcgtgatggc gccggtgcct ggcgtcgcgt gagttacgcg cagatgctgg
     1201 ctcgcataca gcgcctggcc gcagggcttt tgacgatgga tctctcccgc gaccggccca
     1261 ttgccatcct gtccggcaac agcatcgaac atctgacgct ttcgctggcc gcgatgtggg
     1321 tgggcattcc gtactgcccc gtatcgcctg cctactccca ggtgagcacc gatctcgcca
     1381 aactccagta cgtgctggac ctgctcacgc cgggactcgt cgtcgccttc gacacgcccc
     1441 gtttcgcgcg tgcgttatcg ctggtgccgg cgcatgcgaa gatcatcggc gacgcgcttc
     1501 tggatggccg cgcggtgatc gctctcgaat cgctcgaaca agcggtgacc gctgaagtgg
     1561 acgcagctca cgcggccacc gacgccgatt ccatcgtgag atttctgctg acctcaggct
     1621 ccaccggcca gcccaaggca gtgatcacca ccaaccgcat gtgctgcagc aatgccgcca
     1681 tgctgcggca atccatgccg ttcatcgccg gcgaaccgcc cgtgctgctc gactggttgc
     1741 cgtggaatca cacgttcggc ggcagccaca acatcggaat ggtgctggcc aacggcggtt
     1801 cgctgtacat cgacgatggc aggccgaccc ccgcgggtat ctcgcaaaca ctgcagaacc
     1861 tgcgcgagat ttcacccacg gtgtacttca atgtgccgaa aggcttcgag atgctggcgc
     1921 cccatctgcg cagtgatgcg cagctccgca agaccttcta cagcaggctg cgcgcgtact
     1981 tcttcgccgg cgcgtcgctg gcgcagcaca cctgggacga gctcgacgcc gcatcgacac
     2041 tcgaactcgg cttcaaggtg ccgatgttgt cgggactcgg cgcgacggag acgggcccgt
     2101 cggtcacgtt caccactccg gccatgggcc gcagcggcgt aatcggactg ccagcggcag
     2161 gaacgctcgt caaactcgcg ccggtcgaag agaagctgga gatccgggta cgcagtccct
     2221 cggtcacgac gggatattgg cgccagcccg agctcaacgc gcaagcattc gatgacgaag
     2281 ggttctttcg actcggcgat gccgtcaggc tcatcgatcc cgatgaaccg agccgcggat
     2341 tgaaattcga cggcaggatc ggcgaagact tcaagctcgc caatggcacc tgggtcagcg
     2401 tgggtccgtt gcgcgcagag ctgatcagcg cactggcgcc cgtcgcgcaa gatgtggtga
     2461 tcgccggcct cgataccgat ttcgtcgcca tcctcgtgat cccggatatc gatgcctgcg
     2521 catcgatgct cgcctgtcca cacccgccga cgcgcgcgga gctcgccgga gagcccaaac
     2581 tgctgtcggg gattcagcag cgtctcgccg cgcacgcgcg ggcgaatccg gccagcagcc
     2641 gcggtgtgcg cagagcctgc gtactggcgg tcgcgccatc gctcgatcgg ggcgagatca
     2701 cggacaaggg ctcgatcaat cagcgcgcga tgttgcggaa tcacgcggac ctcgtggccg
     2761 agctctattc gcccgcaccc ccgccgcgaa tcgtgatcat cgagatggag aggccatgag
     2821 cgcgcgcgag gaaacagcgg tgaccgtcgg tctgtgtttc ctcgtcgccg tgctcgaagg
     2881 tttcgatatt caggcgatgg gcgtcgcggc gccacgcctg gcgccgcaat tcgggctcga
     2941 tgcggggcag atgggctggg tattcagcat cagcaatttc ggactggtca tcggcgcgag
     3001 cctgggcggc tggctggccg acaggtcggg gcgcaagccg gtgctgatgg gcgcggtcgc
     3061 catcttcgga tgtttcactc tcgcgacggc gtttgccggc gactatcacg cgctgctggc
     3121 ggtgcgtttt ctcgcgggac tcggctttgg cgcagcgttg cccaacatga tggccatcgc
     3181 cgccgagatc agccggccgg agaaggcggc atcgacggcg gccatcatgt tctgtggcat
     3241 gcccgtgggt ggcgggctcg cagcgctgac tacgcagatt cttccagcgg acatcgattg
     3301 gcgcgcgttg ttttatgtgg gtggtgtgct cccggtcctg ctgttgccgg cgctccgcgt
     3361 ctggatgccc gagacgctgg tccggggaac gacacttcaa tcgcgtgacg caagcgtgct
     3421 tggtaccctt ttcggtgaag ggcgagcgac gccgacgttg ctgctctggg tcgcgtttct
     3481 accgactctc ctgatcctct atctattcct gaactggctg ccgacactgg tcatcgcgaa
     3541 gggtctcgag cgggcggtag ccccgcaagc ttcgctcgcc ttcaatttcg gcagtgtggc
     3601 cggcgcgctg gtattcggcg cgttggtgga ccggtttcat ttccgctggc cgttgacgct
     3661 ggcgtacctg ctgttgatcg tggcgttgct gttgctgggt gcatcggcgg gtcacgcggc
     3721 catcattgcg ttgagcggtg cggtggggtt ctgcctgctc ggcgccaact acgcgttgta
     3781 tggtgtcgcg cctgcctact atccacgcgc gatccgcggc acgggctcgg gcgccagtat
     3841 cgccgtcggc cgcatcgggt cgatcgccgg gccgttgctc gcgggcctgt tgatcggtgg
     3901 cggaaccagt gccgccggcg tcgttcaata catggtgccc gtggcagcgg tttccgcgct
     3961 ggccgtcttc gcgctcagct ttcgatcgcg ggcttctgaa acaggccgca catcagggtc
     4021 ctgagccagc ggcttcgggc atcgtggtga aacttgcggt gccagatctg cttcagttcg
     4081 atgctgggtg cttcgaaggg cggccgaatg atgcgaatgc ccgccgacac cgtcgaaaaa
     4141 tatcgggcca gcggcacggg caccgtgacc acgagatctg actgctcgac gatcggcggc
     4201 acgctcgcga aatgcggggt ggtgagcgtg acgcgccgct ggatacggtg acgcgccagg
     4261 aatcgctcga tgacttcttc ggtccggctt tccgcgcgca ccacggcatg gtcgagctgc
     4321 cggaattgct tttgtgtgag ccgcgcagat ttgaccgggt gatccgcgcg gatcagacac
     4381 gtgaagctgt cggagaacag cgtctgctga aagaagttgt gccgcttcaa attcgggaag
     4441 tagccgagcg cgagatcgat cgtgccgctc tcgaggccgc tcgcgacatc ctccgcgggc
     4501 agactcacgg atcgcaggct gacgcccggg gcctcgaggc gcaattgccg caggattgac
     4561 ggcagaaaaa ccacttcgcc gacatcggag agggcgagca cgatcggccg atgactgcgg
     4621 cccggctcga aactgggtgc tgcgacgatg tcagtcccga tgcgcgagag gacttcccgc
     4681 acggctccca tgatttcgac tgcccgcggc gtgggttgca tggtgttgcc gctgcgcacg
     4741 aacaaggggt cggtgaagaa ggttcgcagc ttgcccagcg ccgaactcac cga
//
LOCUS       DATDEU010000677         3062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_715851, whole genome shotgun sequence.
ACCESSION   DATDEU010000677 DATDEU010000000
VERSION     DATDEU010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715851"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VIV63_16170"
     CDS             complement(<1..1811)
                     /locus_tag="VIV63_16170"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY6126190.1"
                     /translation="MNRAFIVLAALVCGGITYSVPSDAADTDWPRYGGDEAKTRHSAL
                     KQIHRGNVKDLALAWQYDTGEKGDTQTQPIVVGRTLYGYSPSHKTFALDATTGKHKWM
                     FDSGIAGVGANRGLMYWAQGAEARVFSAVDNFIYALDATTGKPITTFGIDGRIDLREG
                     LGRDPASQSVRLTSPGVIYRDLMIVGGRVGESLPASPGHVRAYDVRTGALRWSFHTIP
                     QPGEPGYDTWPKDAWQYIGGANSWPGMTLDAKRGVVFVPTGSAAADFYGANRLGDNLY
                     ANCLLALDAATGKLRWHFQFVKHDVLDRDLPTAPTLVTLRVKGKKVDALVQPTKQGFL
                     FVLDRDTGKPVFPIEEVPVPASDVPGEVAYPAYLRPTVHAPYARQRLTADDLTNRTPE
                     ANRWAREQFAQLRSDGPFKPLGTGIDTTIYPGFDGGAEWGGPAYDAATGILYVNSNEM
                     AWLGSLALNDTGRSTGKSIYLRDCAACHGDTGKGSPPQFPSIASFAARMTAEQFAERV
                     RKGGGRMPAFPHIQKEAIQAIDKYLNEGLDIPAGSPDTSPINQAYRFTGYRRWLDPEG
                     YPAVAAPWGKLSAIELATGEFVWSIPFGEFPELAARGV"
     gene            1993..>3062
                     /locus_tag="VIV63_16175"
     CDS             1993..>3062
                     /locus_tag="VIV63_16175"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY6126191.1"
                     /translation="MTRQRTILISLLAVVIVLVVARVAMPYVVKDFVNDKLAALDSYD
                     GQVGDIDIHLWRGAYSIDDIEIVKTGASRPVPFFRSDSVDLSVEWRSLFRGSIVSEVS
                     FFGPELNLVQGKSAADSQLGKEENWNARLEELFPFRFNTIEVTEGTVRFLAPGISTQD
                     AITARRVNGAVSNLTNVVDSGKETFADFKVTADVLEGAPAVVAGSVNAFAAQPTFDVN
                     LEVKKVQLPQVNPWLREYIKADAEAGKFELYMELAAADGKFKGYAKPILQDVNIYRSG
                     EEEQSALKRVWEGFLDFAANVLENPDADQVAARIPFSGTIKDPETSVFATIASVLRNA
                     FISAFARSLEGSITLRDVKKNLG"
BASE COUNT          556 a          911 c         1001 g          594 t
ORIGIN      
        1 acgccacgcg ccgccaactc gggaaattcg ccgaacggaa tgctccagac gaactctccc
       61 gtcgcgagtt ctatggcgct gagtttgccc cagggcgcgg ccactgccgg atatccttcc
      121 gggtcgagcc agcggcgata acccgtgaat cgatatgcct ggttgatggg agacgtgtcg
      181 ggtgatcccg cggggatgtc gagtccttcg ttgagatatt tgtcgatcgc ctgaatcgct
      241 tccttctgga tgtgcgggaa tgccggcatg cggccgccac ccttgcgcac gcgctcggcg
      301 aactgctcgg ccgtcatgcg cgcggcgaag ctcgcaatgg acggaaattg cggtggcgaa
      361 ccctttccag tgtcgccatg gcaggcggcg cagtcacgca ggtagatgga cttgccggtg
      421 ctgcgtccgg tgtcgttgag cgccagcgaa ccgagccacg ccatctcgtt ggagttcacg
      481 tagaggatcc ccgtcgccgc atcgtaggcc gggccacccc attcggcgcc gccgtcgaaa
      541 ccgggataga tagtggtgtc gatgcctgtg ccgagcggct tgaacgggcc gtcgctacgc
      601 agttgcgcaa actgttcgcg tgcccagcgg ttggcttcgg gtgtgcggtt ggtcaggtca
      661 tcggccgtga ggcgctgccg cgcgtagggc gcgtgaaccg tgggacgcag ataagccggg
      721 tatgcgactt cgccggggac gtccgatgcg ggcaccggta cttcttcgat cgggaacacc
      781 ggcttgcccg tgtcacggtc gagcacgaac aggaaaccct gtttggtggg ctgcaccagc
      841 gcatcgacct tcttgccttt cacgcgcagc gtcacgagtg tcggtgcggt cggcaggtcg
      901 cgatcgagca cgtcgtgttt cacgaactgg aagtgccagc gcaatttgcc cgtggcggca
      961 tcgagcgcca gcaggcaatt ggcgtacagg ttgtcgccca agcggttcgc gccgtagaaa
     1021 tccgcggcgg cggaacccgt gggaacgaat accacgccgc gctttgcgtc gagcgtcatg
     1081 ccgggccagc tgttggcgcc gccgatgtat tgccacgcat ctttcggcca ggtgtcgtag
     1141 cccggctcgc caggttgcgg aatggtatga aagctccagc gcaacgcgcc ggtcctgaca
     1201 tcgtaggcgc gcacgtgacc gggagaggcc ggcaggcttt caccgacacg tcccccgacg
     1261 atcatgaggt cgcgatagat gacgccgggg gaggtgaggc gaacggattg cgaagccgga
     1321 tcgcgtccca ggccttcgcg cagatcgatg cggccgtcga tgccgaacgt ggtgatgggc
     1381 tttcccgtgg tggcatcgag cgcatagatg aaattgtcga cggcggagaa cacgcgggcc
     1441 tccgcgcctt gcgcccagta catgagaccg cggttggcgc cgacgcccgc aatgccggag
     1501 tcgaacatcc atttatgttt gcccgtggtg gcgtcgagcg cgaaagtctt gtgcgacggt
     1561 gaatagccgt acaacgtgcg gcccacgacg atgggttggg tctgcgtgtc gcccttttcg
     1621 ccggtgtcgt actgccaggc gagcgcgagg tctttgacgt tgccacgatg gatctgcttg
     1681 agcgcggaat gccgcgtctt ggcttcgtcg ccgccgtaac gcggccagtc tgtgtcagcg
     1741 gcatctgaag gaaccgaata ggtgattccc ccacagacga gtgcggcgag cacgataaac
     1801 gcgcgattca tccgggaccc cctccacctt gtaagaatcg agtgacgcaa tttacggcag
     1861 attgcccctt gggtcacgac gagccggcga gcaaactgca gtcgtgaatc cggacgtgct
     1921 cctcccagcg cgattattgg gttcacacag ggggccgctt agtgcggcct cttttttttt
     1981 ggggggccag tcatgacccg acaacgaaca atcctgatca gcttgttagc cgtggtgatc
     2041 gtgctggtcg tcgctcgcgt ggccatgccg tatgtcgtca aggatttcgt caacgacaaa
     2101 ctggccgcgc tcgattcgta cgacgggcag gtcggcgaca tcgacatcca tctctggcgc
     2161 ggcgcataca gcatcgacga catcgagatc gtgaaaaccg gggcgtcgcg tcccgttcct
     2221 tttttccgat ccgacagcgt ggatctctcg gtcgagtggc gcagcctgtt ccgcggctcg
     2281 atcgtgtccg aggtttcttt cttcggtccc gagctcaatc tcgtccaggg aaagagcgcc
     2341 gcggattctc aactcggcaa ggaagaaaac tggaacgcgc gcctcgaaga actgtttcca
     2401 tttcgtttca acaccatcga agtaaccgaa ggcaccgtac gctttctcgc gccgggcatc
     2461 agcacgcagg atgcgatcac cgcgcgccgc gtgaatggag cggtttccaa tctcaccaac
     2521 gtcgtcgact ccggcaagga gacctttgcc gatttcaaag tgaccgccga cgtgctcgag
     2581 ggtgcgcccg ccgtggtggc cggcagcgtg aacgcgtttg ccgcgcaacc gaccttcgat
     2641 gtgaatctcg aagtgaagaa ggtgcagctt ccgcaggtga atccctggct gcgtgagtac
     2701 atcaaggcgg acgccgaggc cggcaagttc gagttgtaca tggagctcgc ggccgcggat
     2761 gggaaattca agggctacgc gaaacccatc ctgcaggacg tgaacatcta ccgttccgga
     2821 gaggaagagc agagcgcgct gaagcgggtg tgggaaggct tcctcgattt cgccgcgaac
     2881 gtgctcgaga atccggacgc ggatcaggtc gcggcgcgca ttccgttctc cgggaccatc
     2941 aaggatcccg agacgagtgt tttcgccacg atcgcgagtg tgctgcgaaa cgcgttcatc
     3001 agcgcctttg cgcgatcgct cgaaggctcg atcacgttgc gcgacgtgaa gaagaacctg
     3061 gg
//
LOCUS       DATDEU010000678         5664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1007742, whole genome shotgun sequence.
ACCESSION   DATDEU010000678 DATDEU010000000
VERSION     DATDEU010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007742"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VIV63_16180"
     CDS             complement(<1..426)
                     /locus_tag="VIV63_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6126192.1"
                     /translation="MTSTGHETARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGTV
                     KGRKAARHATSDWMALEKQRGISITSSVMQFPFRENIINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDCAKGVEERTIKLMEVCRMRDTPIMTFINKL"
     gene            complement(447..932)
                     /gene="rimI"
                     /locus_tag="VIV63_16185"
     CDS             complement(447..932)
                     /gene="rimI"
                     /locus_tag="VIV63_16185"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY6126193.1"
                     /translation="MAAAPEDLHEVPEVHIRPMLEIDLADVATIEQKSYAFPWSENIF
                     RDCLRVGYTCRALDLAGQVIGYGVMSLGAGEAHILNLCVRDEFRTVGFGRRLLEHMLE
                     RAAAAGISEAFLEVRPSNLAAIRLYQRLGFEQIGIRRGYYQAPDGREDAIVLKLDLRQ
                     P"
     gene            complement(932..1684)
                     /locus_tag="VIV63_16190"
     CDS             complement(932..1684)
                     /locus_tag="VIV63_16190"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY6126194.1"
                     /translation="MGPLRNAYLDALGIDRWVSRNAPPEAIPAEVAPAEVHAAPSLAR
                     APAPSPSTWTPLAPTADWGELRERVAGCTACAELCKTRTQTVFGVGNQSAEWLVIGEA
                     PGAEEDRQGEPFVGRAGQLLNAMLLAIGLPRETVFIANVLKCRPPANRDPKPEEVSRC
                     LPYLSNQIALLKPKIMLAVGRIAAQNLLATDAPLARLRGKLHHFGEANTPLVITYHPA
                     YLLRAPADKRKAWDDLKFARATYARLIGGGNG"
     gene            complement(1694..2467)
                     /gene="pssA"
                     /locus_tag="VIV63_16195"
     CDS             complement(1694..2467)
                     /gene="pssA"
                     /locus_tag="VIV63_16195"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY6126195.1"
                     /translation="MTPDSPTTQNSPRRGIYLLPNLLTTGCLFSGFYSIVAAVDTNFA
                     FAGAAVFVALLFDGLDGRVARWTGTESPFGKEYDSLADMVAFGVAPAVLVYQWGVIRI
                     SEYGDSWRQFGWIIAFFYCACAALRLARFNTRAATGDKRYFEGLPSPSAAATVAAFVW
                     FFSKWREPGLPGLILAFGVTGYAAMLMVSPFSYISMKQIDVDKRVKYSYFGFVILPML
                     ALVFVDPPTMLFAMFISYASYGPVQSLVRKLRRPRAGAA"
     gene            2516..4225
                     /locus_tag="VIV63_16200"
     CDS             2516..4225
                     /locus_tag="VIV63_16200"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY6126196.1"
                     /translation="MRWSQYPVNTTKETPADAEVVSHQLMLRAGLIRKLAAGLYTWMP
                     LGLRAMRKVENIVREEMNRAGALELLMPGVIPAELWVESGRWGEFGPELLRIKDRHER
                     DFCLGPTHEEVITDIARNELKSYRQLPANFYQIQMKFRDERRPRFGVMRAREFLMKDS
                     YSFHIDQASLQAGYDAMHKAYTAIFTRLGLKFRSVVADPGAIGGSGSEEFHVLADSGE
                     DAIAWSDGDNYAANIEMAVALPPATPRAAAGAPLAEVATPGAKTIEQVTQQLKLPATK
                     LVKTLLVDAAEGGVIALLVRGDHELNAVKAQKLPGVAKPLRMSSNDTITKATGAEPGY
                     LGPVGFKGKIYADHSVVALSDFVCGANKKDAHLTGVNWVRDLPEATAADIRNIVPGDP
                     SPSGKGTLAIARGIEVGHIFQLGKKYSESMGATVLDESGKAATLYMGCYGIGVTRVVA
                     ASIEQNHDARGIIWPDAIAPFQLVIVPINAPKSPAVRETAERLYNELLAAGVDVLLDD
                     RDERPGVKFADTELIGIPHRVVVGDRGITAGKLEYRHRRAEAAEEFPLADAITFIRGR
                     LAG"
     gene            4239..4868
                     /locus_tag="VIV63_16205"
     CDS             4239..4868
                     /locus_tag="VIV63_16205"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY6126197.1"
                     /translation="MRKLLPLVALLVPALGFAAPAADAQREPELKSAVQHAIDQAECF
                     EDRYDSAVWYKMMEPRLRKRVPDRAERMDILKTVFCEANRPGETRLPPGLVMAVIEIE
                     SNFNRYAVSYAGAVGLMQVMPFWPESLGMRRHQLIRTPENIRMGCAILRHYMKREKQN
                     VARALARYNGSTGKRWYSDKVIDQWTRWNGADDLGMSGERHAETKPAVR"
     gene            complement(4838..5113)
                     /locus_tag="VIV63_16210"
     CDS             complement(4838..5113)
                     /locus_tag="VIV63_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY6126198.1"
                     /translation="MICRRSFVSGRVQGVFYRATCVRKAESLGLTGYARNLADGRVEV
                     LACGDEATVEVFVAWLWEGSPASKVTDVATGHADAASLQRTAGFVSA"
     gene            5139..>5664
                     /gene="wrbA"
                     /locus_tag="VIV63_16215"
     CDS             5139..>5664
                     /gene="wrbA"
                     /locus_tag="VIV63_16215"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY6126199.1"
                     /translation="MTEILIVYYSRNGATAGLARQAARGVESVAGCTAKIRTVPQLSA
                     ESERPVKPVPDAGAPYATLDDLRAADGLLLGSPTRFGNMAAPLKYFLDGTSSLWLNGA
                     LSGKPAGVFSASQTMHGGQESTLLSMALPLLHHGMLLVGLPFTERGLNQTRTGGTPYG
                     VTHVAGLATQPGVLS"
BASE COUNT         1071 a         1852 c         1818 g          923 t
ORIGIN      
        1 gagcttgttg atgaacgtca tgatgggggt gtcgcgcatg cgacagactt ccatcagctt
       61 gatcgttcgc tcctcgacac ccttggcgca gtcgatgacc atgagcgcac tgtcgaccgc
      121 ggtgagcgtg cgataggtgt cttcagagaa gtcttcgtgg ccgggcgtgt cgagcaggtt
      181 gatgatgttc tcgcggaacg ggaactgcat gaccgaggag gtgatggaga ttccgcgctg
      241 cttctcgagc gccatccagt cagacgtggc gtgacgcgcc gccttgcgac ccttgacggt
      301 gccggcgagc tggatcgcgc ctccgaacaa caggagcttt tccgtcagcg tggtcttgcc
      361 cgcgtcagga tgcgaaatga tggcaaacgt gcgccggcgc gccgtctcat gcccggtcga
      421 agtcattgaa attccggttg gtaactttaa ggttgccgaa gatcgagctt cagcacgatg
      481 gcgtcttcgc gcccatcggg ggcctgataa tacccgcgac gaatgccgat ttgttcgaaa
      541 ccgagccgtt gatagagccg gatggcggcc aggttgctgg gacgcacttc gagaaaggct
      601 tcgctgatac cggcagccgc ggcgcgctcc agcatgtgct cgagcaggcg gcggccaaaa
      661 cccacggtac ggaattcatc gcgcacgcac agattcagta tgtgcgcctc gccggctccc
      721 aggctcatga cgccatagcc gatgacctgc ccggcgagat cgagcgcgcg gcaggtgtag
      781 cccacgcgca gacaatcgcg aaagatgttc tcgctccacg gaaacgcgta gctcttctgc
      841 tcgatggtgg cgacatccgc aagatcgatt tccagcatcg gccggatgtg aacttccgga
      901 acctcgtgca ggtcttcggg tgcggcggcc atcagccgtt tcctccaccg atgagcctgg
      961 cgtaggtcgc gcgcgcgaat ttcagatcgt cccaggcctt gcgcttgtcg gcgggtgcgc
     1021 gcagtagata ggcgggatgg taggtgatga cgagcggcgt gttcgcttca ccgaagtgat
     1081 gcaacttgcc gcgcagccgc gcgagcggcg catccgtggc cagcaggttc tgcgcggcga
     1141 tgcgccccac cgcgagcatg atcttcggct tcagcagcgc gatctggttc gagaggtacg
     1201 gcaggcagcg cgagacttct tcgggcttgg ggtcccgatt ggccggcggc cggcacttga
     1261 ggacgttggc gatgaacacc gtttcgcgtg gcaagccgat ggccagcagc atcgcgttga
     1321 ggagctggcc cgcacgaccc acgaaaggct cgccctgacg gtcttcctcg gcgccgggtg
     1381 cttcgccgat caccagccat tcagcgcttt gattgcccac accgaacacg gtctgggtgc
     1441 gcgtcttgca caactccgcg caggccgtgc agcccgcgac gcgctcgcgc aactcacccc
     1501 agtcagcggt cggcgcgaga ggcgtccagg tcgaaggtga tggcgccggc gccctggcca
     1561 gcgaaggcgc agcgtggact tccgccgggg ctacctccgc cggaatggct tcaggcggag
     1621 cgttcctgga aacccagcga tcgattccga gcgcgtcgag atacgcattg cgcaacggtc
     1681 ccatcggcgc tcctcaggcg gcacccgcgc gcggacgccg cagcttgcgg accagcgact
     1741 gcaccggacc gtacgaagcg taggaaatga acatcgcaaa caacatcgtc ggcggatcga
     1801 cgaacaccag cgcgagcatg ggcaggatca cgaagccgaa gtacgaatac ttcacgcgct
     1861 tgtcgacgtc gatctgcttc atgctgatgt agctgaacgg cgagaccatg agcatggccg
     1921 cgtagcccgt gacaccgaag gcgaggatga ggccgggcag cccgggctcg cgccacttgc
     1981 tgaagaacca gacgaacgcg gccacggtgg cagcggccga tgggctgggc aaaccttcga
     2041 agtaacgctt gtcgccggtc gcggcacgcg tattgaagcg cgccagccgc agcgcggcgc
     2101 aggcgcagta gaagaacgcg atgatccagc cgaattggcg ccagctgtcg ccgtactcgg
     2161 aaatgcggat gacaccccac tgatagacca ggaccgcggg ggcgacgccg aaggcgacca
     2221 tgtcggccag gctgtcgtac tccttgccga acgggctttc cgtgccggtc caccgggcca
     2281 cgcggccgtc cagcccgtcg aacagcagcg cgacgaacac cgcagcgccg gcaaaggcga
     2341 agttggtgtc gaccgcagcg acgatggaat aaaagccgga aaagaggcag cccgtcgtca
     2401 gcaggttggg caacagatat atgccacgcc tgggcgaatt ctgggtcgtc ggggagtctg
     2461 gcgtcacggc cggcatcata ggcgaatcca ccacctgata cactctcgcg cccctatgcg
     2521 ctggtcccaa tacccggtca acacgaccaa agaaactccc gccgatgccg aagtcgtgag
     2581 ccatcagctc atgctgcgcg cgggcctcat tcgcaagctc gccgcaggcc tctacacctg
     2641 gatgcccctc ggacttcgcg ccatgcgcaa ggtcgagaac atcgtgcgtg aagagatgaa
     2701 tcgtgccggt gcgctcgagt tattgatgcc gggcgtcatc cccgccgagt tgtgggtgga
     2761 atccggcagg tggggcgaat tcggccccga gctgctgcgt atcaaggacc ggcacgaacg
     2821 cgacttctgc ctggggccga cgcacgagga agtcatcacc gacatcgcgc gcaacgagct
     2881 caagagctac cgccagctcc cggcgaattt ctaccagatc cagatgaagt tccgcgacga
     2941 gcgccggccg cgattcggcg tgatgcgcgc gcgcgagttc ctcatgaagg actcgtattc
     3001 gttccacatc gaccaggcct cgctgcaggc cgggtacgac gccatgcaca aggcgtacac
     3061 ggccatcttc acgcgcctgg gactcaagtt ccgttcggtc gtcgccgacc cgggcgccat
     3121 cggcggcagc ggttcggaag aattccacgt gctcgcggat tccggtgagg acgccatcgc
     3181 ctggtccgac ggcgacaact acgccgccaa catcgaaatg gccgtggcct tgccgccggc
     3241 cacgccgcgc gccgccgcgg gcgcgccgct cgccgaagtc gccactcccg gcgcgaagac
     3301 catcgaacag gtcacgcagc agctcaaact acccgcgacg aagctggtga agacgctgct
     3361 cgtggacgcc gccgaaggtg gcgtcatcgc gctgctggtg cgtggcgatc acgagctcaa
     3421 cgccgtcaag gcgcagaagc tgccgggcgt cgccaaacca ctgcgcatgt cgtccaacga
     3481 caccatcacc aaggcgaccg gtgcggaacc ggggtatctc ggtccggtcg gtttcaaggg
     3541 caagatctac gccgaccact cggtggtcgc gttgagcgac ttcgtttgcg gcgccaacaa
     3601 gaaggacgcg catctcacgg gcgtcaactg ggttcgcgat ctgcccgaag cgacggcggc
     3661 cgacatccgc aacatcgtcc cgggcgatcc cagcccgtcg ggcaaaggca cgctggccat
     3721 cgcgcgcggc atcgaagtgg gacacatctt ccagctcggc aagaagtaca gcgaatccat
     3781 gggcgcgacc gtcctcgacg aatccggcaa ggcggccacg ctgtacatgg gctgctacgg
     3841 gatcggtgtg acgcgtgtcg ttgcggcatc catcgagcag aaccatgatg cgcggggaat
     3901 catctggccg gacgccattg cgcccttcca gctggtcatc gttccgatca acgcaccgaa
     3961 atcacctgct gtccgtgaaa ccgccgaacg cctctacaac gagctccttg ccgcgggtgt
     4021 cgacgtcctg ctcgacgatc gcgacgagcg gccgggcgtc aaattcgcgg acaccgaact
     4081 catcggcatt ccgcatcgcg tggtcgtggg cgatcgcggc attacggccg gcaagctcga
     4141 ataccgtcac cggcgcgcgg aagcggccga agaattcccg ctcgccgacg ccatcacgtt
     4201 catccgcggc aggctcgcgg gttagcgagt cgcggggcat gcgcaaactc ctgcccctcg
     4261 tcgccctcct cgtccctgcg ctcggcttcg cagcaccggc cgcggacgcg caacgcgagc
     4321 ccgaactcaa gagcgcggtt cagcacgcga tcgaccaggc ggaatgtttc gaggaccggt
     4381 acgactccgc ggtctggtac aagatgatgg agccgcgcct gcgcaagcgc gtgcccgatc
     4441 gcgccgaacg catggacatc ctcaagactg tgttctgcga ggccaatcgc cccggcgaga
     4501 cgcgccttcc tcccggcctc gtcatggcgg tcatcgagat cgaaagcaat ttcaaccggt
     4561 acgccgtgtc gtatgcgggc gcggtgggcc tcatgcaggt catgccgttc tggcccgaga
     4621 gccttggcat gcggcgccac cagctcatcc gcacgccgga aaacatccgc atgggttgcg
     4681 ccatcctgcg tcactacatg aagcgcgaga aacagaacgt cgcgcgcgca ctcgcgcggt
     4741 acaacggcag cacgggcaaa cgctggtatt cggacaaggt catcgatcag tggacgcgct
     4801 ggaacggcgc ggacgacctc ggcatgagcg gcgaacgtca cgccgaaacg aagcccgccg
     4861 ttcgctgaag tgatgccgca tcggcgtgcc cggtggcgac gtcggtaact ttcgaggccg
     4921 gcgacccttc ccacagccag gcgacgaaca cctcgaccgt ggcctcatcg ccgcaagcca
     4981 gcacttcgac gcggccgtca gcgagattgc gcgcgtatcc cgtcaacccc agcgactcgg
     5041 ccttgcgcac gcaggttgcg cgatagaaaa cgccttggac tcggcccgaa acaaaagaac
     5101 ggcggcagat catgcggcat catatccacg aggatcagat gaccgaaata ctcattgtct
     5161 attactcccg caatggcgcc acggccgggc tcgctcgtca ggcggcgcgc ggcgtcgaga
     5221 gcgttgccgg ttgcaccgcg aaaatccgca ccgtgccgca gttgagcgcg gagagcgaac
     5281 ggccggtcaa acccgtgccc gatgccggag cgccctacgc cacgctggac gacctgcgcg
     5341 cggccgacgg attgttgtta ggcagcccga cgcgcttcgg caacatggcc gcgccgctca
     5401 aatactttct cgatggcaca tcgagcctgt ggctcaacgg cgctttgtca gggaaacccg
     5461 cgggtgtttt ttccgcatcg cagacgatgc acggtggaca ggagtcgacg ctgctgtcga
     5521 tggccttgcc gttgctgcat cacggaatgc tgctggtggg gctccccttc accgagcgcg
     5581 gcctcaatca gacgcggacc ggcggcacac cgtatggcgt cacgcatgtc gccggtctcg
     5641 ccacgcagcc cggcgtcctg tccg
//
LOCUS       DATDEU010000679         8697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1445980, whole genome shotgun sequence.
ACCESSION   DATDEU010000679 DATDEU010000000
VERSION     DATDEU010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445980"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1065)
                     /locus_tag="VIV63_16220"
     CDS             complement(34..1065)
                     /locus_tag="VIV63_16220"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6126200.1"
                     /translation="MEAVDVRGRFVWQQLMTRDVPGARKFYSKLMGWKAQPWPPDPAY
                     TVCLAGDVPVAGIMEIPADLPTEVPAHWLEYIGTRDVDGTADAAVRAGGSIVKAPTDL
                     KGAGRYALLMDPQGALFAIIDPENARPEPAGMPPVGAFSWHELATSDHEAAFAFYSGL
                     FGWDAITRMDMGPAGVYLIFGVNGVQRGGMYIKPPDWPAPPNWLPYGNVPSVDDSAAE
                     LEAAGARILNPPMEVPGGSRIATLIDPAGAAFAINSTPAAAAPAAAPTAKPKAKAKAK
                     AKPRAKPKAKVAVKAKAAKKKAPARKKAAKKVARKVARKVVKKTAKKKAKAKTKMKAK
                     KSKGARRKK"
     gene            complement(1124..1846)
                     /locus_tag="VIV63_16225"
     CDS             complement(1124..1846)
                     /locus_tag="VIV63_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126201.1"
                     /translation="MLKNVIAIIIAVAVGALGYSWWTNKDAEESPLNVMVTQMRTRAI
                     IEHERNVTVWYKACPEVKGINPQVMVIWPAKLNYELDLAETKMSLSADGVLHVSTPPI
                     RADDPSVPSEYGEYMAQTSFWTLDSEQAIVMREMQKATPLARHLTAYFLRNDAGLSAH
                     FKEELETYLRGIAGALGVVVTKIDIEIPEVEVQIPARPELTLCEGSIALANGLGFARR
                     QADGTVIGIYPKKSAAKTSPAP"
     gene            complement(1857..3038)
                     /locus_tag="VIV63_16230"
     CDS             complement(1857..3038)
                     /locus_tag="VIV63_16230"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY6126202.1"
                     /translation="MVDAVIVSTARTALAKSWRGAFNLTHGATLGGHVVKHAMERAKL
                     DPAEVEDVLMGCANPEGATGFNIARQIALRAGCPVTTSGMTVNRYCSSGLQTIALAAQ
                     RIMAGEGDVYVAGGVESISCVQNEHLNMFMAKEAWLEKNKPEIYWNMVQTAETVASRY
                     QISREAQDRYGVQSQQRAAAAQAAGRFTEEIVPIDVIAGVADRDTGRLETRKVTVSAD
                     EGIRPDTTYEAVSKIRTATPGGVIAAGNASQFSDGASACVVMSAARAARAGLTPLGAF
                     RGFAVAGCEPDEMGIGPVFAIPKLLERAGKTVADIDLWELNEAFAVQVLYCADKLGIP
                     NERLNVNGGAIAVGHPYGMTGARLAGHALIEGKRRGAKLAVVTMCIGGGMGAAGLFEI
                     F"
     gene            complement(3031..5133)
                     /locus_tag="VIV63_16235"
     CDS             complement(3031..5133)
                     /locus_tag="VIV63_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY6126203.1"
                     /translation="MTAVRYKVDKGIAWLAMNNPPLNALSHELRKGLVAALDRAIADT
                     RVRAVVIIGNERAFSSGADIKEFAGNAFYAEPFLPAVVDIVEAAPKPVIAAISGACMG
                     GGLELALGCHYRVALASAKIAFPEVKLGLIPGAGGTQRFPRLVGLEAAVNMIVSGATV
                     PASTFNGTALFDVVVPDSLENAARDFAQKLIKEAKPIRRVRDLPCKHPQAEAFLQFAK
                     TGVASVSRGLAAPTRALEAVGAAVEKKFDAGIETEQKIFGELMVSEESKALRHAFFAE
                     RAASKVAGLTDKTPQRKIAQVAIVGAGTMGGGIAMNFANVGIPVIILEAKQEALDRGI
                     AGIRKQYEGAVTKGKLKADDAAKRAALITPSLDYAAASKADLVIEAVFEDIGVKKAVF
                     QALDKVMKRGAILATNTSTLDVDRIAAFTKRPGDVLGMHFFSPANIMKLLEVVRAKKT
                     RPDVLATVLSLARRIGKTAVVSGVCDGFIGNRMINQYSSQALLLLEEGASPSQVDRAV
                     EKFGFAMGPFRMSDLAGNDISWHIRKRHYAEHPKQRKMRIADRVCELGRFGQKTGLGW
                     YRYEPGKRDAIPDPVVDKIIDEERKALKIEPRKIPDAEIVDRLLYALVNEGAHILEEG
                     IAQRASDIDVVYLAGYGFPVQRGGPMFHASRTGLGQVVRRMKEFGWKPAKLLVQLAAA
                     GKTFDDVPPAKKKGGRRG"
     gene            complement(5195..6610)
                     /locus_tag="VIV63_16240"
     CDS             complement(5195..6610)
                     /locus_tag="VIV63_16240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY6126204.1"
                     /translation="MSAVPSSLPAQRIDAAGKPHVDLREVFKLAAPLMATNAVQALLN
                     LTDLWFIGRLSTDAVAAMGAIYWIMSCAILLFGGVGLAVQTFVAQAYGSRRGARASRA
                     LWNAMWASVALMPLFVGLAFAGPFLLKPFGLDPHVEALALEYWKPRMFGAFIGSMGWA
                     AMGFFNGISAVRFTMAVVVTTTVANAIFNQIFMFELGMGMKGAAWGTNAAQLIGVLLA
                     MTLFLRGEVARRYRSKLMWRPRWSVVRAQLVVGMPIGVMYGADVLGLALSQMMIAQTG
                     ATGAAATQIVMALTSMAYMPTIGVALAGTTLVGQSIGAGDREWANRLGNVVIIMCATL
                     MALVAVLLLVLGPWVVPLFVSSGGADAADIVALGLVLLWPAAAYQIFDGLYCGSSFCL
                     RGAGDTRVPAATALVLSWFLFVPLAHTLVFAPGQGWIDGLPQYGLGAKGGWLALMTYC
                     TLLGCSMLWRWRHGAWRRVKL"
     gene            complement(6607..7887)
                     /locus_tag="VIV63_16245"
     CDS             complement(6607..7887)
                     /locus_tag="VIV63_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126205.1"
                     /translation="MTTPAPTVPVGREERRALLMAAAGFFCLLCGYYTLRPMREALAL
                     EVGVQYNSILFSVVLVCSAALLPIYWWMVGRTPRGRLLWYVCTPFAAVFVALALGLHW
                     YPRDKTLAFVYFVSLTSANLYVISVFWSAMADVWRPELAKRFYGYIAAGGSAGALLGP
                     FIVTSLVRTVGPTPLILFACVSIMATAGLVSAARASLRRSAHGQEVPDAAIPVGGRAL
                     DDLGRLVRTPYLLGIAGVIIAGQTIGAFMYNEQGKYVAEAYSAVADRAAIFANMEIAV
                     NLLSLFFQAVVVTWLTRRGSVALSLSAMPALIGASFIALAMFPLGSVLLVTQVLRRAA
                     DYGLGKPPREMLFTVLNPESKFKSKSLIDTVLQRGADSAAQWLYVLIAGIGLVGIAWF
                     CAGLCVLLLAATRMLGRAFETRQQPKMGTLDPAS"
     gene            complement(7884..8369)
                     /locus_tag="VIV63_16250"
     CDS             complement(7884..8369)
                     /locus_tag="VIV63_16250"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY6126206.1"
                     /translation="MLAVDDHLGQPRWDETCTFLARKRVAPGAGKTTTISRAIENGGE
                     GLTIKSSSFRAPAGLGRRYGFISGDVNPIHMSDLTARAFGFPKAIAHGMWSLGRLASD
                     FEADQFNGGCELNVNFKLPIYMPAWLMLQRWDIENGAAFALRDAQGEKPHLTGTLKAL
                     R"
     gene            8349..>8697
                     /locus_tag="VIV63_16255"
     CDS             8349..>8697
                     /locus_tag="VIV63_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126207.1"
                     /translation="MVIDRKHLAQYSEICGASAGATLPIAYPHILAMPLHLAMLGAEA
                     FPVKLFGLVHVQNRIAMREPLSADEAAEIRCWIEGHRDTERGQEFDLHTEYVVGGQPR
                     WDETCTFLARKRVA"
BASE COUNT         1535 a         2902 c         2723 g         1537 t
ORIGIN      
        1 ctagtcctgc cttgatgacg tctcgacgtc ctgtcatttt ttcctcctcg cgcctttgga
       61 tttcttcgct ttcatctttg tcttcgcctt tgcctttttc ttcgccgttt tcttcacgac
      121 cttgcgtgcg actttcctgg cgactttctt cgcggccttc tttctggccg gcgccttctt
      181 cttcgcagct ttcgctttga ccgcgacttt ggctttaggt ttcgccctgg gcttggcttt
      241 tgcttttgct ttcgcttttg gtttcgctgt gggtgcggcg gccggagccg ccgccgccgg
      301 cgtcgagttg atcgcgaacg cggcaccggc cggatcgatc agcgtggcga tgcggctgcc
      361 accgggcact tccatcggtg gattcaggat cctcgcgccc gccgcttcga gttcggccgc
      421 gctgtcatcg acgctgggca cgttgccata cggaagccag ttcggcggcg ccggccagtc
      481 aggcggcttg atgtacatgc caccgcgctg aacgccgttc actccgaaga tgagatagac
      541 accggccggt cccatgtcca tgcgcgtgat ggcatcccag ccgaacaggc cgctgtagaa
      601 cgcgaatgcc gcttcgtgat cgctggtggc gagttcatgc cacgagaacg cgccgacggg
      661 cggcatgccc gcgggttccg ggcgcgcatt ttccgggtcg atgatggcga acaacgcgcc
      721 ctgaggatcc atgaggagcg cgtaacggcc cgcccccttc aggtcggtcg gcgccttgac
      781 gatcgagccg cccgcacgca cggccgcatc ggcggtgcca tcgacatcac gcgtgccgat
      841 gtactccagc cagtgcgctg gaacctcagt cggcaggtcg gcggggatct ccatgatgcc
      901 ggccaccgga acgtcgcccg cgaggcatac cgtgtaggca ggatcgggtg gccatggctg
      961 ggctttccat cccatgagct tcgagtagaa cttcctggcg cctggcacat cacgtgtcat
     1021 cagctgttgc cagacaaatc ggccgcgcac atcgacagct tccatggacg gatctccgtt
     1081 gtgtgtggac ggcgatcata gcgccgcgac gggcgctttt gtcttacggc gccggtgatg
     1141 ttttcgcggc ggatttcttt ggatagatgc cgatgacggt gccatccgcc tgacgccgcg
     1201 cgaatccaag tccattggcg agcgcaatgg acccttcaca caacgtgagc tcggggcgcg
     1261 cgggaatctg cacctcgacc tcgggaattt cgatgtcgat cttcgtgacg acgacaccga
     1321 gcgcgccggc gatgccgcgc aggtaagtct ccagttcttc cttgaagtgc gcgctcaaac
     1381 ccgcgtcgtt gcggagaaaa taggcggtga ggtgccgcgc gagcggcgtg gctttctgca
     1441 tttcccgcat gacgatggcc tgctcgctat ccagcgtcca gaacgaggtc tgcgccatgt
     1501 actcaccgta ttccgacggc acggacggat cgtcggcacg gatgggcggc gtgctcacgt
     1561 gcaagacacc atcggcgctc agcgacatct tcgtttcggc gaggtcgagc tcgtagttga
     1621 gcttcgcggg ccagatcacc atgacctgtg gattgatgcc tttgacctcg gggcaggcct
     1681 tgtaccagac cgtcacgttg cgctcgtgct cgatgatggc gcgcgttcgc atctgcgtga
     1741 ccatgacgtt gagcgggctt tcctcggcgt ccttgttcgt ccaccacgaa tagcccaggg
     1801 cgccgaccgc cacggcgatg atgatcgcga tcacgttctt gagcacgtca actccttcaa
     1861 aagatctcga agaggccggc agcacccatt ccgccgccga tgcacatggt aaccacggcg
     1921 agtttcgcac cgcgccgttt accctcgatc agcgcatgac ccgccaggcg cgcacccgtc
     1981 atgccatagg gatgcccaac cgcgatcgca ccgccattca cattgaggcg ctcattggga
     2041 atgccgagct tgtcggcaca atagagcacc tgaacggcga acgcttcgtt gagttcccac
     2101 aagtcgatgt cggcgacggt cttcccggcg cgctcgagca gtttcgggat cgcgaacacc
     2161 gggccgattc ccatttcatc cggttcgcag cccgccaccg cgaaaccgcg gaacgccccg
     2221 agcggcgtaa gcccggccct cgccgcccgc gcagcgctca tgacgacgca ggcggacgcg
     2281 ccgtcggaga actggctggc gttgcccgcc gcgatgactc cgccgggcgt cgccgtccgg
     2341 atcttcgaga cggcttcgta ggtggtgtcg ggtcggatac cttcgtccgc gctcactgtg
     2401 accttgcggg tctcgaggcg tccggtgtcg cgatccgcga ctccggcgat cacgtcgatg
     2461 gggacgattt cctcggtgaa ccgcccggcg gcctgcgccg cggctgcacg ctgctggctc
     2521 tgaacgccgt atcgatcctg tgcctcgcgc gaaatctggt agcggctggc cacggtctcc
     2581 gcggtctgca ccatgttcca gtatatttcc ggcttgttct tctcgagcca ggcttccttc
     2641 gccatgaaca tgttcagatg ttcgttctga acacaggaga tggattcgac gccgcccgcc
     2701 acgtacacat caccctcgcc ggccatgatt cgctgcgcgg caagcgcgat ggtctgaagg
     2761 cccgacgagc agtaacgatt cacggtcata cccgacgtgg tgacggggca tccggcacgt
     2821 agcgcgatct ggcgcgcaat gttgaaaccc gtggcgccct cgggattcgc gcaacccatg
     2881 agcacgtcct cgacctcggc gggatcgagt ttcgcgcgtt ccatggcgtg tttgaccacg
     2941 tgcccaccga gcgtcgcgcc gtgcgtgaga ttgaacgctc cgcgccagga cttggcgagc
     3001 gcggtgcgcg ccgtggaaac gatgacggca tcaaccacgg cggcctcctt tcttcttcgc
     3061 gggcggcaca tcgtcgaaag tttttcccgc ggccgcgagt tgcacgagga gcttcgccgg
     3121 cttccagccg aattccttca tgcgcctgac tacctggccg agacccgtgc ggctggcatg
     3181 aaacatcggg ccgcctcgct gcacgggaaa gccataaccc gcgagataga ccacgtcgat
     3241 gtcggaggcc cgctgcgcga tcccctcttc gaggatatgc gcaccttcgt tgacgagcgc
     3301 gtacagcagc cggtcgacga tctcggcatc gggaattttc cgcggctcga tcttcaacgc
     3361 cttgcgctcc tcgtcgatga tcttgtcgac cacgggatcc gggatcgcgt cgcgtttgcc
     3421 cggctcgtag cgataccaac cgagtccggt cttctgcccg aagcgcccga gttcgcagac
     3481 ccggtcggcg atgcgcatct ttcgctgctt cggatgttcg gcgtagtgac gtttgcggat
     3541 gtgccagctg atgtcattgc cagcgagatc cgacatgcgg aacggcccca tggcgaaacc
     3601 gaatttctcg acggcgcgat ccacctgcga aggactcgcg ccctcctcca gcagcaacaa
     3661 cgcctggctc gaatactggt tgatcatgcg attgccgatg aagccatcgc acacacccga
     3721 gacgaccgcc gtcttgccga tgcgccgcgc cagcgacagc accgtggcca gcacgtcggg
     3781 ccgcgtcttc ttcgcgcgca ccacttccag caacttcatg atgttcgcgg gactgaagaa
     3841 atgcatgccg agcacgtctc ccggccgctt cgtgaacgcc gcgatgcggt cgacatcgag
     3901 cgtcgaggtg ttcgtggcga gaatcgcgcc gcgcttcatc accttgtcga gcgcctggaa
     3961 cacggccttc ttcacgccga tgtcttcgaa cacagcctcg atgacgagat cggctttgga
     4021 tgccgcggcg tagtcgagtg atggcgtgat caacgcagcg cgtttggcgg cgtcgtccgc
     4081 cttgagcttg cccttggtca cggcgccttc gtattgcttg cgaatgcccg cgataccacg
     4141 gtcgagcgct tcctgcttcg cttccaggat gatcacgggg atgccgacgt tcgcaaaatt
     4201 catggcgatg ccgccgccca tggttccggc gccgacgatc gcgacctgcg cgatcttgcg
     4261 ttgcggcgtc ttgtcggtga gccccgccac tttcgaagcc gcgcgctcgg cgaagaatgc
     4321 atggcgcagc gccttcgact cctcgctgac catgagttcg ccgaatatct tctgctcggt
     4381 ctcaatgccc gcgtcgaact tcttctcgac cgccgcgccg acggcttcca gcgctcgcgt
     4441 gggtgcggcg aggccgcgcg acacggatgc caccccggtt ttcgcgaact gcaggaacgc
     4501 ttcggcctgg ggatgtttgc agggcagatc acgcacgcgg cgtatcggtt tcgcttcctt
     4561 gatgagcttc tgcgcgaaat cgcgcgccgc gttttccaga gagtccggta cgaccacatc
     4621 gaacagtgcc gtgccattga acgtggacgc gggaactgtc gcgcccgaga cgatcatgtt
     4681 caccgccgct tcgaggccga cgagccgcgg gaagcgctgc gtcccgccgg cgccggggat
     4741 gagaccaagc ttgacctcgg ggaacgcaat cttggcgctg gccagcgcca cgcggtaatg
     4801 acaccccagc gcgagctcga ggccgcctcc catgcaggcg ccgctgatcg cggcgatcac
     4861 cggtttcggt gcggcttcga cgatgtcgac gacggcgggc agaaacggct ccgcgtaaaa
     4921 cgcattgccg gcgaattcct tgatgtcggc gccgctcgag aaggcgcgct cattgccgat
     4981 gatcaccacg gcgcgcaccc tggtgtcggc gatggcgcgg tcgagcgcgg ccacgagacc
     5041 cttgcgcaac tcgtgcgaca acgcattcag cggtggattg ttcatcgcca gccaggcgat
     5101 gcccttatcg actttgtagc ggacggccgt catggtccga tcataagcac ggcggcgagc
     5161 acgagtcgat caggcaacac ccgcaacgtg acgtctacag tttgacgcgc cgccaagcgc
     5221 catgccgcca gcgccagagc atgctgcagc ctaacaacgt gcagtatgtc atcagggcga
     5281 gccatccacc cttggcaccc agcccgtatt gtggcaggcc gtctatccag ccctgccccg
     5341 gcgcgaagac cagtgtgtgg gcgagaggca cgaacaggaa ccatgacagg accagcgcgg
     5401 tcgccgcggg aacccgcgta tcgccggcgc cccgcagaca gaagctcgat ccgcagtaca
     5461 agccatcgaa gatctggtag gccgcggcgg gccacaacaa caccaggccg agtgcgacga
     5521 tgtcggccgc atccgcgccg ccactgctca cgaacagcgg caccacccac ggccccagca
     5581 cgagcagaag aaccgccacc agcgccatga gcgtggcgca catgatgatg acgacattgc
     5641 ccagacgatt tgcccattcg cggtcgccgg cgccgatgga ctgtccgacc agcgtcgtgc
     5701 ccgcgagggc cacaccaatg gtgggcatgt aggccatgga cgtgagcgcc atgacgatct
     5761 gcgtcgcggc cgcaccggtg gcgcccgtct gcgcgatcat catctgtgac aacgcaagcc
     5821 cgagcacgtc ggcgccgtac atcacgccga tgggcatacc gaccacgagc tgtgcgcgca
     5881 ccaccgacca gcgtggacgc cacatcagct tcgagcgata gcggcgggcc acttctccgc
     5941 gcaggaacag cgtcatggcc agcaacacgc cgatgagctg cgcggcgttg gttccccatg
     6001 ccgcgccctt catgcccatc ccgagctcga acatgaagat ctgattgaaa atggcattgg
     6061 cgaccgtcgt ggtgaccacc acggccatcg tgaagcgcac ggccgagatg ccgttgaaga
     6121 accccatggc cgcccagccc atcgacccga tgaacgcgcc gaacatgcgg ggcttccagt
     6181 actcgagcgc cagcgcctcg acatgcgggt ccagcccgaa aggcttcagc aggaacggcc
     6241 cggcgaacgc cagcccgaca aacagcggca tgagagcaac cgacgcccac atcgcattcc
     6301 acagcgcgcg cgaggcacgc gcgccgcgac ggctgccata ggcctgcgcc acgaaggtct
     6361 gcaccgccag tcccacgcca ccgaacaaca ggatggcgca gctcatgatc cagtagatgg
     6421 cacccatggc cgcgaccgca tcggtggata gccggccgat gaaccacagg tcggtcaggt
     6481 tgagcagcgc ctgcacggcg ttggtcgcca tgagcggtgc ggcgagcttg aagacttcgc
     6541 gcagatcgac gtgcggcttg cccgcggcgt cgatgcgctg cgccggcagc gacgatggaa
     6601 ccgcgctcat gacgccggat ccagcgtccc cattttcggc tgctgacgcg tttcaaaggc
     6661 gcgcccgagc atgcgcgtcg cggccagcag caggacgcac agcccggcgc agaaccaggc
     6721 gatgccgacg agaccaatgc ccgcgatgag gacgtacagc cattgcgctg ccgagtcggc
     6781 tccgcgctgc aggaccgtgt cgatgagact cttgctcttg aacttgctct cgggattgag
     6841 cacggtgaac aacatctcgc gcggaggttt gccgagcccg taatcggcgg cgcgccgcaa
     6901 tacctgtgtg accagcagca cgctgcccag cggaaacatc gcgagcgcga tgaaactcgc
     6961 gccgatgagc gcgggcatgg cggaaagact gagtgcgacg ctgccgcgtc gcgtgagcca
     7021 ggtgaccacc acggcctgga agaacaacga cagcaggttt accgcgatct ccatgttcgc
     7081 gaagatggcg gcgcgatccg cgacggcgga atacgcctcg gcaacgtact tcccctgctc
     7141 gttgtacatg aacgcgccga tcgtctgccc ggcgatgatc acgcccgcga tgccgagtag
     7201 atagggcgtg cgaaccaggc ggccgagatc gtcgagcgcc cggccgccga ccgggatcgc
     7261 ggcatcgggc acctcctgtc catgggccga ccggcgcagg gaagcgcggg ccgccgacac
     7321 caatccggcc gtcgccatga tggaaacgca ggcgaagagg atgagcggcg tcggtccgac
     7381 ggtacgcacc agcgacgtca cgatgaacgg gccgagcagc gcaccggcgc tgccaccggc
     7441 ggcgatgtag ccgtagaagc gtttcgcgag ctcgggccgc cagacgtcgg ccatcgcgct
     7501 ccagaatacg gagatcacgt agaggttcgc cgaggtgagc gaaacgaaat agacgaaggc
     7561 cagggtcttg tcgcgcgggt accagtgaag acccagcgcc agtgcgacga ataccgcggc
     7621 aaacggcgtg cacacgtacc acagcaggcg tccgcgcggc gtacgcccga ccatccacca
     7681 gtagatgggc aacagcgcgg cggaacagac cagcaccacg ctgaagagga tcgagttgta
     7741 ttgcacgccc acttcgagcg cgagcgcctc gcgcatcggc cgcagcgtgt agtaaccgca
     7801 cagcaggcag aaaaaccccg cggccgccat caacaatgcg cgccgctcct cccggcccac
     7861 cgggactgtc ggcgccggcg tcgtcaccgc agggctttca gcgttccagt caggtggggc
     7921 ttttcgccct gggcgtcgcg aagcgcgaac gccgcgccat tctcgatatc ccagcgctgc
     7981 agcatcagcc atgccggcat gtagatgggc agcttgaaat tgacgttgag ctcgcagccg
     8041 ccattgaatt ggtcggcctc gaagtccgag gccagccggc cgagcgacca catgccatgc
     8101 gcgatcgcct tgggaaagcc gaaagcgcgc gcggtgagat cactcatgtg aatgggattc
     8161 acgtcaccgg agatgaagcc ataacggcgc ccgagaccgg ccggggcgcg aaagctgctc
     8221 gacttgatgg tcaggccttc gccgccgttc tcgatcgcgc ggctgatcgt ggtggttttt
     8281 cccgcgcctg gcgccacgcg cttgcgcgcg aggaacgtgc aagtctcgtc ccagcgcggc
     8341 tggccaaggt ggtcatcgac cgcaagcatc tcgcgcaata ttcggaaatc tgcggcgcat
     8401 cggccggcgc cacgttgccc atcgcctatc cgcacatcct cgccatgcct ctgcacctgg
     8461 ccatgctcgg cgccgaggcg ttcccggtga agttgtttgg cctcgtgcac gtgcagaacc
     8521 gcatcgcgat gcgcgagccg ttgtcggccg atgaagccgc ggaaatccgc tgctggatcg
     8581 aaggtcaccg cgacaccgag cgcggccagg aattcgacct gcacaccgaa tacgtggtcg
     8641 gcggtcagcc gcgctgggac gagacctgca cgttcctcgc gcgcaagcgc gttgcgc
//
LOCUS       DATDEU010000680         7206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_571959, whole genome shotgun sequence.
ACCESSION   DATDEU010000680 DATDEU010000000
VERSION     DATDEU010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571959"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VIV63_16260"
     CDS             <1..922
                     /locus_tag="VIV63_16260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6126208.1"
                     /translation="GGSINYQGKNSNAQVLGVPDEYFMVKEDVPFNFGRRPNPLDQDE
                     IRKVVVIGTVVAERLFAKGEDPVGKYVRVKNVEMKVVGVFYDKGGRGQNSERVLMPLT
                     TMQKMYGDSRFTDNIWVRPEKGVDGFELEKKVLELLKRRHEVAPEDIRGIASFNMAEP
                     MKRVTGLFIGIDVFIWFVGLGTLTAGIVGISNIMIITVKERTREIGIRKALGATPFHI
                     VSTLLLESTLVTGFAGYLGLVVGVGLLELMAFGLRSSGAKLPYFMNPSVDFQLALTAL
                     ALLIGVGVLAGLMPALRAAKITPTEAMRAD"
     gene            932..2197
                     /locus_tag="VIV63_16265"
     CDS             932..2197
                     /locus_tag="VIV63_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6126209.1"
                     /translation="MFIDIEKWQEIFSTLRRHKLRTALTAFGVFWGIFMLTVLLGAGK
                     GLENGVAEGFPKTTNTVWIWQQGTTQIPFQGMPIGRAIGLKPDDVQAIAKNVASVGFI
                     TGQNSVGVWDNAPPFTVRKARNGAFSIQGGFAAMDSINSVRIIQGRSFNPLDEQQKRK
                     VAVIGQRVRDQLFAKDENPLGEDITINGITFQVIGVFRSTQNGNQQQEEERIYLPNDT
                     LRYAFNQIGRIGSFVIVPKPGIHARVAENDVRMYLSQINKVSPDDRGVFGSFNLQEEH
                     DKVQGLFTGIKVFSWMVAIGTIFAGAVGVGNIMLIVVKERTREIGLRKALGATPASIV
                     AMIMQESVFITAVAGYSGLVVATLLLESIASALNAAGGRAGFMGPPEVEFQTAIIALA
                     VLVASGLLASLMPAAKAAAVNPIVALQDE"
     gene            2202..3275
                     /locus_tag="VIV63_16270"
     CDS             2202..3275
                     /locus_tag="VIV63_16270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6126210.1"
                     /translation="MKRIIKFLVLGLIAVVFIGTFVFLYRKSQVQPVVFQVDSPAKMT
                     IIKKTVATGKVIPRREVEVKAQVSGVVDRLYVAAGQTIKKGAIIARISLRPSMLNVSA
                     AEAQVLSARINLRNQTLELERNKKLLAQKLISDSQFNLVQTNFDLQKEAVSSAEDTLL
                     LLKTGSSKNSGNVSNMIPATIDGTVLDLPFKEGAFILESSTFQSGTTLATLADMNDMI
                     FEGLVDESEVGKLRQGMELILNVGALQGKPFKATLEYISPKGVTDQGTIKFTIRAAVQ
                     LDKELFLRSNYSANADIVLDKREDALAVNEGNLIIEDKATFVEVETAPQKFEKREIKT
                     GLSDGINIEVVSGIKPEDKIKHR"
     gene            complement(3291..5009)
                     /locus_tag="VIV63_16275"
     CDS             complement(3291..5009)
                     /locus_tag="VIV63_16275"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEY6126211.1"
                     /translation="MDTPKPSKRARSKVARKIRRVVDAAQRLTTTASTPNSRVSRPEA
                     LAGYQAAVEPLPSRAHLSVRAMPDEVRASLQSLKASDRTFHGAKFSLAYFRSSRTTSP
                     CADMFGYLSAARIRQNTRLPFDATSRALLEELGGLMGDPGRDVAPDSVIPAGYTYVGQ
                     FVDHDITMDVSSNLDVMTDANTIHNMRTPSLELDSVYSRGPALDPFFYVFPTVGNPTA
                     IKFRLGTNTNTGPGGPGGSAGLPGMKVQVDFDVPRISAPAHTAIVGDPRNDENLIVSQ
                     FHQTILRFHNAVVDMLLGSGFAGDIFIEAKRLVTHHYQWAVVHDFLKRICGQAVVTQA
                     INSSSVAKGSPFRMPVEFAVAAYRFGHSMIRDNYWVNFNFPNATLAQVFEFNRNPRLP
                     VFSNWVVDFNAFFDTGVPVPINNKARKIDSVMAAGLESLPGFAGMMQVLAIRNLRRGL
                     ALGLPSGQGMAKQFKVAPLTEQQLVQGLPAAEVAILQKQNKLLLKKTPLWYYVLRESA
                     VTTGGDRLGPVGARIVARTFAQILKRDAASYLNAPTPFAPTLTAATAGDFTFADLVTF
                     AKVTQP"
     gene            5233..6030
                     /locus_tag="VIV63_16280"
     CDS             5233..6030
                     /locus_tag="VIV63_16280"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEY6126212.1"
                     /translation="MNENIKWFAAAVGVVGLSVGAVIYLSMKDKAPPPTEPPLAVTPP
                     PAAVPEEEPAVKHPLPTPANQEALPALADSDQPMQNAIGDLIGKETVEKYFVPENLVR
                     HLVVSIDNLSESKIAERIRPLKRVPGSFEVDGADQTLVLDPRNYERYKPLVQTIKAVD
                     SKVLIATYVRYYPLFQDAYESLGHPPEYFNDRLIEIIDLLLATPDVRSPIPLAQPGVL
                     YEYADPKLEALSAGQKALIRMGSDNAAAVKDKLRELRVELVAQKPAD"
     gene            complement(6051..>7206)
                     /locus_tag="VIV63_16285"
     CDS             complement(6051..>7206)
                     /locus_tag="VIV63_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126213.1"
                     /translation="TGTPEEAAAWVNYANKVKRHNIRYWEIGNENYGTWEADNNTRPH
                     DPVTYATRFKEYWLQMKAVDPTIKIGAVVVLGEDAFANYPDLQVTNPRTGVAHSGWTP
                     VMLDTLKQLGVTPDYVVYHRYEQGPGGESDLFLLNSASSWRNDSNALRQILDDYLGSK
                     AKLVELAVTEHNSVFSNPGKQTTSLVNGLFYADALGNLLLTDFNALLWWDLRNGQEAG
                     NNNSPSLYGWRRYGDYGIVNAADPAGAADRYPTFYVNKLLKYFARGGEKVVKAGADYG
                     SLGVYAVTGADRSMRILVINKHPSATLNANIDIPALKRGEKATVYSYGIPQDEAARTG
                     EGSADVQQSTLTISGTTLRFSPGPYSAHVIQLAPRARAKGAKSDAWCDDE"
BASE COUNT         1390 a         2187 c         2268 g         1361 t
ORIGIN      
        1 cggcggctcc atcaactacc agggcaagaa cagcaacgcc caggtcctcg gtgtgcctga
       61 cgagtacttc atggtcaagg aagacgtgcc gttcaatttc ggacgcaggc ccaatccgtt
      121 ggaccaggac gaaatccgca aggtcgtcgt catcggcacc gtggttgccg agcgattgtt
      181 cgccaagggc gaagacccgg taggcaagta tgttcgcgtc aagaacgtgg aaatgaaggt
      241 cgtgggagtc ttctacgaca agggtggtcg cggacaaaac tcggagcggg tcctgatgcc
      301 gctgaccacc atgcagaaga tgtacggcga ttccaggttc accgacaaca tctgggtgcg
      361 tccggaaaag ggcgtcgacg gtttcgagct cgagaagaaa gtcctcgagc tgctcaagcg
      421 acgccatgag gttgcgccgg aagacatccg cggcatcgct tctttcaaca tggccgagcc
      481 catgaagcgt gtcaccgggc ttttcatcgg catcgacgtg ttcatctggt tcgtcggctt
      541 ggggacgttg acggcgggca tcgtcggcat cagcaacatc atgatcatca ccgtcaagga
      601 gcgcacccgg gagatcggta tccgcaaggc gctcggtgcg acgccgtttc acatcgtcag
      661 tacattgctg ctcgagtcca cgctggtaac cggattcgcc ggctacctgg ggctcgtagt
      721 gggggtaggc ctgctcgagc tgatggcatt cggcctgcgc agctccggcg cgaaactgcc
      781 gtatttcatg aatcccagcg tcgacttcca gcttgcgttg acggcacttg cgctgctgat
      841 aggcgtgggc gttctcgcgg gactgatgcc ggcgctgcgc gccgcgaaga tcacgcccac
      901 cgaagccatg agggcggatt gacgcggcgc catgttcatc gacatcgaaa aatggcagga
      961 gatcttcagc acgctgcgcc ggcacaagct gcgcaccgcg ctcaccgctt tcggcgtgtt
     1021 ctggggcatc ttcatgctga cggtgttgtt aggcgccggc aaggggctcg agaacggtgt
     1081 cgccgaaggt tttcccaaga ccaccaacac cgtgtggatc tggcagcagg gtacgacgca
     1141 gattccattc cagggtatgc cgatcggacg cgcgatcgga ctcaaacccg acgacgttca
     1201 ggcgatcgcg aagaatgtcg cctcggtggg attcatcacc ggtcagaatt cggtcggtgt
     1261 ctgggacaat gcgccgcctt tcaccgtgcg caaggcccgc aacggtgcgt tcagcatcca
     1321 gggtggtttt gccgccatgg acagcatcaa ctcggtgcgc atcatccagg gccgttcatt
     1381 caacccgctg gacgagcagc agaagcgcaa ggtggccgtc atcggccagc gggttcgcga
     1441 ccagttgttc gccaaggacg agaacccgct gggtgaggac atcaccatca acggcatcac
     1501 cttccaggtg atcggcgtgt tccgttcgac ccagaacggc aaccagcagc aggaagagga
     1561 acggatctat ctacccaacg acacgctgcg ctatgccttc aaccagatcg gcaggatcgg
     1621 cagtttcgtg atcgtgccca agcccgggat ccacgcccgc gttgccgaga acgacgtccg
     1681 gatgtatctc tcgcagatca acaaggtcag ccccgacgac aggggggtct tcggcagctt
     1741 caacctgcag gaagaacacg acaaggtgca gggcctgttc accggcatca aggtattcag
     1801 ctggatggtc gccatcggca ccatcttcgc gggcgccgtg ggcgtcggaa acatcatgct
     1861 gatcgtggtg aaggaacgta cgcgcgagat aggcctgcgc aaggcgctgg gcgcaacacc
     1921 cgcctccatc gtcgccatga tcatgcagga gtcggtgttc atcaccgcgg tggcgggtta
     1981 cagcggcctg gtggtcgcca cgctgctgct cgaatccatc gccagcgcgc tgaacgcggc
     2041 cggtggccgg gcgggtttca tggggccacc cgaagtcgaa ttccagactg cgatcatcgc
     2101 gctggcggtg ctggtggctt ccggattgct ggcttcattg atgccggcgg ccaaagcggc
     2161 cgccgtgaat cccatagtgg ctttacagga cgagtagaag aatgaaacga atcatcaagt
     2221 tcctcgtgct cggcttgatc gcggtggtgt tcatcggcac cttcgttttc ctgtaccgga
     2281 aatcgcaggt ccagccggtc gtcttccagg tggatagccc ggcgaagatg accatcatca
     2341 agaagacggt ggccactggc aaagtgattc cgcgccgcga agtcgaagta aaggcgcagg
     2401 tgtcgggcgt ggtcgacagg ctgtacgtgg ccgccggcca gaccatcaag aagggcgcca
     2461 tcatcgcgcg catctcgctg cgcccgagca tgctcaatgt gagcgcggcc gaggcgcagg
     2521 tgttgtccgc caggatcaac ctgcgcaatc agacgctgga gctcgagcgc aacaagaaac
     2581 tgctggcaca gaaactgatc tccgactcgc aattcaatct cgtccagacc aacttcgatc
     2641 tgcagaagga agcggtgtcg tcggccgaag atactttgct gttgctcaag accggatcat
     2701 cgaagaactc ggggaatgtc tcgaacatga ttcccgccac catcgatggc accgtgctcg
     2761 atctgccttt caaggaaggc gcgttcatcc tggagtccag cacgtttcag tcgggcacca
     2821 cgctcgcgac gctggccgac atgaacgaca tgatcttcga gggcctcgtc gacgaatcgg
     2881 aagtgggcaa gttgcgccag ggcatggaac tcatcctcaa cgtcggcgcg ctgcaaggta
     2941 agcccttcaa ggcgacgctg gagtacatct caccgaaagg cgtgaccgac cagggcacga
     3001 tcaagttcac catccgcgcg gcggtgcagc tcgacaagga actattcctg cgatcgaact
     3061 acagcgccaa cgcggacatc gtgctcgaca agcgggaaga cgctttggcc gtcaacgaag
     3121 gcaacctcat catcgaagac aaggccacct tcgtcgaagt cgaaacggcg ccgcagaagt
     3181 tcgagaagcg cgagatcaag accgggctgt ccgatggcat caacatcgag gtcgtctcgg
     3241 gcatcaagcc cgaggacaag ataaaacacc gctgacctgc gccgacgtcc tcaaggctgc
     3301 gtcactttcg cgaaagtgac caggtccgca aacgtgaagt cgcctgcggt ggccgccgtc
     3361 agcgtcggcg cgaacggcgt gggcgcgttc agatacgacg ccgcatcccg cttgaggatc
     3421 tgcgcaaagg tccgcgccac gatgcgggcg ccgaccggcc ccagcctgtc tccgcctgtc
     3481 gtgacagccg attcgcgcag cacgtagtac cacagcggcg tcttcttcag cagcagcttg
     3541 ttctgcttct gaagtatcgc cacctcggcg gcgggcagac cttgcaccag ctgctgttcg
     3601 gtcagtggcg ccaccttgaa ttgtttggcc atgccttgac cgctgggcaa tcccaacgcc
     3661 aggccgcgcc gcagattccg tatcgccagc acctgcatca tcccggcgaa tcccggcagg
     3721 ctttcgaggc ctgcggccat gacgctgtct atcttgcgcg ccttgttgtt gatgggcact
     3781 ggcacgccgg tatcgaagaa ggcattgaag tcgaccaccc agttggagaa caccggcagg
     3841 cgcggattgc gattgaactc gaatacctgg gcgagcgtgg cattcggaaa gttgaagttc
     3901 acccaatagt tgtcgcgaat catgctgtga ccgaagcgat acgccgcgac ggcgaattcc
     3961 acgggcatgc ggaacggact gcctttggcc acgctgctcg agttgatggc ctgcgtgacc
     4021 acggcctggc cgcaaatgcg cttcaggaaa tcgtggacca ccgcccactg ataatgatga
     4081 gttacgagcc gcttggcctc gatgaagatg tcgcccgcga agccagaccc gagcagcatg
     4141 tcgaccacgg cgttgtggaa tcgcagtatc gtctgatgaa actgcgagac gatcaggttt
     4201 tcatcgttgc gtggatcgcc cacgatcgcg gtgtgcgccg gcgcggagat gcgtggcacg
     4261 tcgaaatcca cctggacttt catcccgggc aagccggcgc tgcccccggg cccgccaggt
     4321 ccggtgttcg tattcgtgcc gagccggaac ttgatggcgg tcggattgcc gaccgtcgga
     4381 aacacataga agaacggatc gagcgcgggt ccgcgtgagt acacgctgtc gagctcgagc
     4441 gaaggtgtcc gcatgttgtg gatggtgttc gcgtccgtca tcacatcgag gttcgacgat
     4501 acgtccatgg tgatgtcgtg atcgacgaac tgaccgacgt aggtgtatcc ggcggggatc
     4561 actgaatccg gcgcgacatc gcgtcccgga tcgcccatca acccaccgag ctcctcgagc
     4621 aatgcgcggc tcgtcgcgtc gaacggcagt cgggtgttct ggcgaatccg cgccgccgac
     4681 aggtatccga acatgtcggc acacggcgaa gtggtgcggc tgctcctgaa gtaggccagc
     4741 gagaatttcg cgccgtgaaa ggtccggtcg gaggccttca gcgactggag cgacgccctg
     4801 acttcgtcgg gcatggcgcg cacggacaga tgcgcccgcg agggcagcgg ttccacggcc
     4861 gcctggtagc ccgccagcgc ttcgggccgg gagactcgcg aattgggcgt cgatgccgtg
     4921 gtggtcaggc gttgcgccgc atcaacgacg cggcgaatct ttctggcgac ttttgacctg
     4981 gcgcgcttgc tcggcttggg ggtgtccatg tttgcgtctc ccgcttcttg tgattgacag
     5041 tccgcgggca ggggttgtta ggcggtccca actttcgcac tgcccaccag ggatctgtcg
     5101 ccgcgcgcgc gtccattgca gtgcggctgg agaaaatccg cttaaccggg gcggcacatc
     5161 gaaaaccgcc ccaacaggga gcaatcgggg cgggcagggg aacgatccgt gcgaattcct
     5221 gtcaggttcc tcatgaacga aaacatcaag tggtttgccg ccgcggtggg cgtggtcgga
     5281 ctttcggtcg gcgcggtcat ctatctgtcg atgaaggaca aggcgccacc gcccaccgag
     5341 ccgccgctgg ccgtgacgcc gccgcccgcg gcagtgccgg aagaagagcc ggctgtgaaa
     5401 catccgcttc caacgcccgc gaatcaggag gcgttgcccg cactcgcgga cagcgatcaa
     5461 ccgatgcaga acgccatcgg tgacctgatc ggcaaggaga ccgtcgagaa gtatttcgtc
     5521 cccgagaacc tggtccggca tctcgtggtg agcatcgaca acctctcgga aagcaagatc
     5581 gccgaacgca ttcgtccgtt gaagcgcgtg cccggcagtt tcgaggtgga cggcgcggac
     5641 cagaccctgg tgctcgatcc gcgcaattac gaacgctaca aacctctcgt gcagacgatc
     5701 aaggctgtcg actcgaaggt tttgatcgcg acctatgtcc ggtactaccc gctgttccag
     5761 gacgcgtatg aaagcctcgg gcatccgccg gagtacttca acgatcgcct gatcgagatc
     5821 atcgacctgc tgttagcgac gcccgacgtg cgcagtccca ttccgctcgc gcagccgggc
     5881 gtgctttatg aatatgccga tccgaagctc gaggcgctgt ccgcgggcca gaaggcgctg
     5941 atccgcatgg gcagcgacaa cgcggcagcc gtcaaagaca agctgcgcga actccgtgtc
     6001 gagctcgtcg cgcaaaagcc cgcggactaa ccgcgggctt cgcggcgctg tcactcgtcg
     6061 tcgcaccacg catccgactt cgcgcccttg gcacgcgcgc gaggagcgag ctggatcacg
     6121 tgcgccgaat acggtccggg tgaaaacctg agcgttgttc ccgaaatcgt gagcgtcgac
     6181 tgctgcacgt cggccgaacc ttcgccagtg cgcgccgctt catcctgcgg aatgccgtag
     6241 ctgtacaccg tcgccttctc gcctcgcttc aaggccggta tgtcgatgtt ggcattgagc
     6301 gtcgcggacg gatgtttgtt gatgaccagg atgcgcatgc tccggtcggc acccgtcacc
     6361 gcgtatacgc cgaggctgcc gtaatctgct ccggccttca cgaccttttc gccaccgcgc
     6421 gcgaagtact tgagtagttt gttgacgtag aacgtcgggt acctgtcggc ggcacccgcg
     6481 ggatcggcgg cattgacgat tccgtagtcg ccgtagcggc gccagccgta cagcgacggg
     6541 ctgttgttat tgccggcttc ctggccattg cgcaggtccc accacaacag cgcgttgaaa
     6601 tccgtcagca gcaggttgcc cagggcgtcg gcgtagaaca ggccgttgac gaggctggtg
     6661 gtctgcttgc ccgggttgct gaacacggag ttgtgttcgg tgaccgcgag ctcgacgagc
     6721 ttggccttgg agccgaggta gtcgtcgagt atctggcgaa gcgcgttcga gtcgttgcgc
     6781 cagctcgagg ccgagttcag caggaacaga tcgctttcgc cgcccggtcc ctgttcgtag
     6841 cggtgataca ccacgtagtc aggcgtgacg ccgagttgtt tcagcgtgtc cagcatgacg
     6901 ggcgtccagc cgctatgagc cacgcccgtg cgcgggttgg tgacctgcag atccgggtag
     6961 ttggcgaacg catcttcacc gagcaccacg accgcaccga tcttgatggt gggatcgacc
     7021 gccttcatct gcagccagta ttccttgaag cgcgtggcat acgtcaccgg gtcgtgcggc
     7081 cgcgtgttgt tgtccgcttc ccacgtgccg taattctcgt tgccgatttc ccagtaccgg
     7141 atgttgtggc gcttgacctt gttcgcgtag ttgacccagg ccgccgcttc ttccggcgtg
     7201 ccggtg
//
LOCUS       DATDEU010000681         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1591092, whole genome shotgun sequence.
ACCESSION   DATDEU010000681 DATDEU010000000
VERSION     DATDEU010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591092"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VIV63_16290"
     CDS             complement(<1..421)
                     /locus_tag="VIV63_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY6126214.1"
                     /translation="MDSLVNRHITIKSAAEQEKMRVAGRLAAEVLDMIGEHVKPGVTT
                     EELDRLCFEHITEVQKSIPANVNYRGFPKTICSSVNHVVCHGIPNDKVLKSGDILNID
                     VTVIRDGFHGDTSRMYYAGNPSVMAKRLVDTTREAMWR"
     gene            632..1357
                     /gene="rpsB"
                     /locus_tag="VIV63_16295"
                     /pseudo
     CDS             632..1357
                     /gene="rpsB"
                     /locus_tag="VIV63_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            1673..>2444
                     /gene="tsf"
                     /locus_tag="VIV63_16300"
     CDS             1673..>2444
                     /gene="tsf"
                     /locus_tag="VIV63_16300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY6126215.1"
                     /translation="MNIPADAVKQLRERTGAGMMECKKALVEANGDLDAAAENMRKSG
                     LAKADKKASRVAAEGVVVIEKSADGKTGVLVEVNSETDFVARGDDFRGFAAEVAKATL
                     AANATSAEAVSALKLASGETVDEKRRALISKLGENLTVRRVVLVTAPTIVGSYVHTDN
                     KKAALVALEGGDADLARDLAMQVVAASPRYLTPADAPAEHVAKEREIETAKALAEGKP
                     AEIVAKMIEGRLRKSLNEFALTGQPFVKDADVTVDKLLK"
BASE COUNT          485 a          753 c          807 g          399 t
ORIGIN      
        1 cgcgccacat cgcctcgcgg gtcgtgtcga cgaggcgctt ggccatcacc gacgggttcc
       61 ccgcgtagta catgcggctg gtgtcgccgt ggaacccgtc gcggatcacg gtcacgtcga
      121 tgttcaggat gtcgcccgac ttcaggacct tgtcgttcgg aatgccgtgg cacacgacgt
      181 ggttcacgga cgagcagatg gtcttcggga aaccccgata gttcacgttg gccggaatgg
      241 acttctgcac ctcggtaatg tgctcgaaac acaggcgatc gagctcctcg gtggttacac
      301 cgggcttcac gtgctccccg atcatgtcga ggacctcggc ggccaggcgg ccggcgacgc
      361 gcattttttc ctgttccgcc gcggacttaa tggtgatgtg gcggttcacg agggagtcca
      421 tcgggggtac ctgcgtaagg cattgttttg acgggggagg gatggtataa agcgcggcct
      481 tttttggcaa tgaaactcac ttaaaccaca cgcgcaacct gcgggtctgt ctctccagac
      541 ggcctgcata acgggtggat gggtgtcccg ggagcaatcc aaggatatcc acccgggcgg
      601 cgcggaggct taacccacca ggagaaattt gatgtccggc gtgtcaatgc gacaaatgct
      661 ggaagcgggc gtccattttg gacatcagac ccgcttctgg aacccgaaga tggctccgtt
      721 cattttcggc gaacgacaca agatccacat catcaacctc gagaagacgc agccgatgta
      781 ttcggaagct gcggccttcg tgaagagtgt ggtcgcggac ggcggcaaag tgctgttcgt
      841 cggcaccaag cgctccgcgc gcgaagccgt cgagaaggaa gctcgccgtt gcgagatgcc
      901 ctacgtcaac cagcgttggc tcggcggcat gctcaccaat ttcaagacca tccgcggctc
      961 gatcaagcgc ctcgccgacc tcaccgagat gaccgaaaac ggcacgctcg gcaaacgcgg
     1021 caagaaagaa ggcacgatga tgcgccgtga gaaggacaag ctcgagaaga gcctgggcgg
     1081 catcaagcag atggaatcgc ccccggacgt catcttcatc atcgacgtcg gtcatgaaga
     1141 aatcgcgatc ctcgaagcga agaagctcgg catcccggtg gtcgcggtgg tggacaccaa
     1201 ctgcgccccg gacaacgtgg actacgtgat cccgggtaac gacgacgcca tgcgcgccat
     1261 ctctctgtat gccgctggta tcgccgattc ggtcatcgaa ggccgctcgc aggccccggc
     1321 cgttgccgtg ggcgaagacg aattcgtcga gctcgacgag aacggcaatc cgcgcaagaa
     1381 aggcgcacgt gctgctccgc cgaaggcgcc gccgccgcag cgtcgaggcc gcccggccaa
     1441 caccgcgacc cgcggccgcc ggccggtggt tgcaagcgtc gcagccgatc cggaagacgg
     1501 tgccgatccg gccgtgttca cggacacggg cgaagcggct ccgcgcagcg caactgccag
     1561 cgccggccgt tcgcgcacgg gtacgggcaa cgccccgggc cgtggtcgtg gcggcggtgg
     1621 tgggccgcgt cgcggctgat cgctcctttc gaaagttttg aggtaagtac gaatgaacat
     1681 tcccgcagat gcggtcaaac agctgcgtga gcgcaccggc gccggcatga tggagtgcaa
     1741 gaaggcactc gtcgaagcga atggcgacct ggacgctgcc gccgagaaca tgcgcaagtc
     1801 cggtcttgcg aaggccgaca agaaggcctc gcgggtcgcg gccgaaggtg tcgtggtcat
     1861 cgagaagtcc gccgacggca agaccggcgt gctggtggaa gtgaacagcg agaccgactt
     1921 cgtcgctcgt ggtgacgact tccgtggctt cgctgccgaa gtcgccaagg cgacactggc
     1981 cgccaacgcg acgagtgccg aagccgtgag cgcgctcaag ctcgccagtg gtgagaccgt
     2041 cgacgagaag cgtcgcgcgc tcatttccaa gctcggcgag aacctgaccg ttcgacgcgt
     2101 agtcctcgtg acggcgccga ccatcgtggg ttcctacgtg cacaccgaca acaagaaggc
     2161 ggcgctcgtg gcgttggaag gcggcgacgc tgacctcgcg cgcgatctcg ccatgcaggt
     2221 ggtcgcggcc agcccgcgtt acctgacccc ggcggatgcg ccggcggaac acgtcgcgaa
     2281 agagcgcgag atcgagaccg cgaaggcgct tgccgaaggc aagccggccg agatcgtcgc
     2341 caagatgatc gaaggccgcc tgcgcaagtc gctcaacgag ttcgcgctca cgggacagcc
     2401 gttcgtcaag gacgccgatg tcacggtcga caagctgctc aagg
//
LOCUS       DATDEU010000682        11808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1446033, whole genome shotgun sequence.
ACCESSION   DATDEU010000682 DATDEU010000000
VERSION     DATDEU010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446033"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VIV63_16305"
     CDS             complement(<1..203)
                     /locus_tag="VIV63_16305"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126216.1"
                     /translation="MIYLIDASVYVFRAYYSMPPDMVDSDGNPAHATFGFARFLGDLI
                     ERAQPRYIAVAFDASLTTSFRNQ"
     gene            complement(275..991)
                     /locus_tag="VIV63_16310"
     CDS             complement(275..991)
                     /locus_tag="VIV63_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126217.1"
                     /translation="MNSADVFSLEFDMLTRTSQLGKILERFRATEIGAGTHRQLLLEL
                     NHVINVHSRLREVAADVVPPDDWTSWQDLLAVPALAFEQGRSFLDDLRALGLNSMDSV
                     SLTPLPQTIALAAYAHYQLVERATVGLVGYLWFFERMPRLLYPLWGESCRRGGVPEKA
                     LRALAEGAVVQSARDQVVANCCRQIVRRPRDLGLATQSLHDTAELFATMLDAALLRAE
                     RRIPMVESVVTVGTGIGQVA"
     gene            1222..4491
                     /locus_tag="VIV63_16315"
     CDS             1222..4491
                     /locus_tag="VIV63_16315"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY6126218.1"
                     /translation="MTRKITLTRLVSGLCAILVLTLSACTGSFEPEPLVEYSTEVRID
                     GAKPLKFSRQLRQGAYLVEIRERDIDLRIHIDAGDQHTELADAYQRHGLHRTVVSLAE
                     PGSVRVTLTSVDQRNWRGAAAVRILRWPSRPPDAPPDERLLGYVALGKANALIARADT
                     DSWRASIHPMRQAAAHFAAANDMQALAEAEYQRSAVELNLLFEFDDGRRTAEAAQAHF
                     VAADDATGEARAAVLRARNEFSIAAAMGADVPRGEQRALLDAAVRRTQQAQAYFETND
                     LHTDAIAALYVSCIREQVLGEYERAAPVYRAIRARARERGDKLFEVRATQNLAFIAAR
                     RGNVAQSVAMLDEVLPLIDRDRNPDLYARLISNLGSGLIALGDFDRALMLHSDALELF
                     SARGDVTQTAREFAALASIQFRSGNVERALDTIESALPLYERGNDQSDYISALHLAGN
                     AAAELGRHEAALQYLRRAEHQDRYGIALDRTRVLIAGELRATGDLRGAERLLSQVLLT
                     RSEPTRADALAERARLRELQRRNPEALADLREADNIYARLKLDFNRIDTSSALAMALL
                     AAGDTQSARRAADTAVAIETRIRVSSANPELRARFLSASYAPYEARIEAEIAGAAPRD
                     PAAIWQAFRVAEAIRARSLTDRLAQTRSVIGQPRDEVTEKLRERMTGLQVELEHRMRK
                     EEVDDSEIHETRRLIDEVSARLETRELRQRATRSTSQLAIAESLDAVQAALPADTAVL
                     AYFVGDRKSHGWLLSRSGLRHAVLPGRRELGDLVEAFVIRQRDGAKAPDNRAFAPLLG
                     NLLSGVSARRLLVLPDGPLNGLPFAALPLPDGRAQDLLIDRFVVSSAPSLALALHTVA
                     RTQAAQTRVAVISDPVYTPDDRRLTVAATETVNYRGGEGTTERLARLPYSAIEARAVV
                     RAFKGADVIELAGFNATARRVIELPSEGLDVLHFATHAVARRDVPGQSALFLSEFAAD
                     GSRLANNRLTLDDIARSGLRADVVVLSGCATGDGRELRGEGVLGLTYGFLANGSHTVV
                     ASLWPVEDALTARFMEEFYATYRVSGSAPEALRIAQLRARGTSGPAVWSSFVVRANEL
                     P"
     gene            4757..8035
                     /locus_tag="VIV63_16320"
     CDS             4757..8035
                     /locus_tag="VIV63_16320"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEY6126219.1"
                     /translation="MSVRDHVATYLRRILRLRTLGLMAIGGVLCISACTDGSKTDVLP
                     EFSAELNLSGDSASPTARSLQAGVYLVEAREVEIDMHVTVIAGGTRSELEDRIPRHGA
                     IYKIVSLASPGTLEVQIRSVDHRTKQGRVQLRIGRWNRPQQSPPGERELGYTAFGDSE
                     EQVALATAESATRAVDKLHEAITHFEAADDESMRAQAAFSLAYLQSEGRDQWAAAVRA
                     SDIATDAYEAVDDEAGIHNAAIVRASSEIKLAGDMNASTQRAEQRAMYAGAERRLTDA
                     ATYFGAHGQPVWAQYAINMRAVLAANLGDYAGAGKFLEQSVKMARDNNDVAEQAKSLA
                     NLAAIHAYLGFVAQAAQEYEALLPLIDPQVQPYQYAALLGNLGYTLIALGDFDRALSL
                     RTQALELYTKIGEEEERALELAALGGLYFRMGDAERALEALRSAIVEQERISDTVGLA
                     STLRVTANVASVLGQHDTALGYLRKSALIDANPHTVARTRVLIAQELRELGNLAAAEA
                     ELVSALESGNALVRASALEERAHLRVAQKKPASAIDDLRAADQQYAALGLEFNRIDTN
                     TALSQALLGARDIKGAAGAARDAIAIVSRIRTKSANPEWRAHFLSARYAPYEALIAAE
                     LAAADDGAPWRTFRIAEEVRARSLADELAAGVIRQADPREDELRARLTDQQLRLESRM
                     QRQDADEAGTLALRHSIEETRAQLDANRLRSGGVAAREASIPDSLQKVQKQLPANTAV
                     LAYFVGDRSTHAWLLTGRELRHATLPGREPLQRAADAAVAARRSGQGVQAEHQLSSML
                     LGQLLDGISETRMLVLADGPLNGVPFASLPLARSGNEILVDRFVVGYAPSLALAMGNS
                     RSSKSSNTRVAVISDPVYAPDDRRLHLAKGGGNGNLRGLPSTAPNKLTRLPYSALEAS
                     AVTRAFGATDTIQLSGFDATAERVLKLPTQDLAVLHFATHALARQDLPDQSALYLSEY
                     AKDGSVLPATRLTASDISRGGLRADVVVLSGCATGDGSALRGEGVLGLTYGFLANGSH
                     SVVATLWPIEDASTARFMNEFYLAYRASGRAADALRAAQIRTRGSAAPGVWSSFVVRA
                     NEFP"
     gene            8089..8640
                     /locus_tag="VIV63_16325"
     CDS             8089..8640
                     /locus_tag="VIV63_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126220.1"
                     /translation="MYGKLKTGGRIFGIWLAAATLALGASVAAAQERAPDEALSGWLG
                     KEFTVESSTLNDHMPTGGKLTFVFDAEDNVVRICTRNSTRQRNSWSMDFATPCGVTML
                     FTRGSRYCTTDDVKTGDAEVLSSCHRLRSRDIAMRPARVKGTVELNDMIAFLVKDGTG
                     KPVVSILVDSPARVTDGGIILLK"
     gene            8686..10203
                     /locus_tag="VIV63_16330"
     CDS             8686..10203
                     /locus_tag="VIV63_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY6126221.1"
                     /translation="MLSLAAAATLGGSCALAAAPNLCSHDGRWAAASTRAPAPVLEYL
                     YSLGDARFDLRAACAQWAGMFSQRHQSAADSAQQSRRIPARLIVFPAEGVNQRELVEA
                     LKKTGYVRMIERSRNFVVVLLSSLTPTARLVSIINSPQVGYFEPDCDGPDYLTTSMDL
                     PTAVSAQSHCWQRAARKTFPNDPCLDELWGHQKIGWDSAVAARSLPRIVAVLDSGIDA
                     AHEDLARNVVLNLASPEAPYSQGGRLNARCATSGRCFPHGTEMAGTIGGRMDNGIGVA
                     GVAPNSLLLPIVISRVESGLLARISTIAAGIDAAVEDGAEIINISAKWPVDSRAISES
                     IVAAVGGQSAVRRLVITGYATSLDRDDTVREYFPSQYRCLAGVIAAVPTDMRGQELFN
                     PKGHPDAKDGRIHAPGVDIVVTTTENSERGYALSGAAGASSAAAYVSGVVALVWGSPP
                     LNKCDAQQIKQLLFCRSKSSNSTRYPWVNVDFLRELANVDPAVDCPGALAALGCN"
     gene            10228..10896
                     /locus_tag="VIV63_16335"
     CDS             10228..10896
                     /locus_tag="VIV63_16335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6126222.1"
                     /translation="MSTLPLDEIERIVAAALTGDRAAESRMLVALRPGVLAVLRFGAF
                     HRWIDAEDLTQETLHIVVERVRAGTIDDPRKVFAFAAATARNLALNAARKMLRQQTVV
                     DSELIDELAQNLEMEQSDLSDSDDRHLAQAVAELLEELPTERDRQLLTRFYLDGADKQ
                     LLCRDLGLSPKHFDRVLMRARSRLRTIIERRAPHLALFPRISSILLPLTISITFWVRH
                     FGSW"
     gene            10909..11661
                     /locus_tag="VIV63_16340"
     CDS             10909..11661
                     /locus_tag="VIV63_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126223.1"
                     /translation="MKSLDRNSLEEQTRLVSRYVAGDLSRSERAEFEAWLVASPELAA
                     EVEMERRLRRGIASAARRGWLKRSTPVERMRDRNWQVALAASVLVSVGLALSILVPRE
                     AASPAGNRVASASQRNAPARTVRLSSVRGGASAPDVSFSIADAPGALTIEPDVVVLTC
                     ADGAIELECADGHSPQQPQYAEYEMDLVNRRGATLAWRSSRQAPVGRTQLSFTLRDPG
                     SLDAGDYDVIVRGHSPEHEEVVARFWLRVAPK"
BASE COUNT         2077 a         3888 c         3810 g         2033 t
ORIGIN      
        1 atctggttgc gaaacgaggt cgtcaggctc gcgtcgaacg ccaccgcgat gtagcgcggc
       61 tgcgcgcgtt cgatgagatc gcccaggaat cgcgcgaaac cgaaggtggc atgcgccggg
      121 ttgccgtcgc tgtcgaccat gtcgggcggc atggagtagt aggcgcggaa gacgtagacc
      181 gacgcgtcga tgaggtagat cacgcgcgga tggtaaatgg ggtcagaccc catttcctag
      241 caattggggt ctgaccccat tttttccatt tttttcaggc gacttggccg atcccggtgc
      301 cgacggtgac gacgctctcc accatgggaa tccgccgttc ggcgcgcagc aacgcggcgt
      361 cgagcatggt cgcgaacaac tcggcggtgt cgtgcaggct ctgggtcgcg aggccgaggt
      421 cacgcggccg ccgcacgatc tgccggcagc agttcgccac gacctgatcg cgcgccgact
      481 gcacgacggc gccctccgcc agtgcgcgca gcgccttttc cggcaccccg ccgcgccggc
      541 acgactcacc ccacagcggg tacagcagcc gcggcatgcg ctcgaagaac cagaggtaac
      601 ccaccagccc taccgtggcg cgttcaacca gctggtaatg ggcataggcg gcaagcgcaa
      661 tggtctgtgg caatggagtc aacgacacac tgtccatgga gttgagtccg agtgcgcgca
      721 gatcgtcaag aaaggagcgt ccttgctcaa atgccagcgc tggaacggct agcaggtcct
      781 gccaggaagt ccaatcgtcg ggcggcacga catcagccgc gacctctcgc aaccgcgaat
      841 ggacgttgat cacatgattg agctcgagca acagctggcg gtgagtgccc gcaccgatct
      901 cggtggcacg aaaccgctcg aggatttttc cgagttgcga cgtgcgcgtc agcatgtcga
      961 actcgaggga aaagacgtcg gcgctgttca tcctgagcct gttattcccg ggcgttttgg
     1021 tccggtcact atgtagtgtc acgacttcag ggtttctctc acctcgcgct gtcataacga
     1081 caaagtattt tcaatcaagg acttactaca aaccctgcct actttttcta ttcttgattg
     1141 cggcacgacg caggtgcctg taaacattgg catcgaagcc aaggggatct cgccagaatg
     1201 tgcgtacgca ccggcgttgg gatgacgaga aaaattacgc tcacacgact cgtctcgggt
     1261 ctgtgcgcca ttttggtcct caccctatct gcctgtaccg gcagcttcga acccgagccc
     1321 ctggtcgaat attccaccga agtccgcatc gacggcgcca agcctctgaa gttcagccgc
     1381 cagttgcgcc agggcgcgta cctggtcgag atccgcgagc gcgacatcga cctgcgcatc
     1441 cacatcgatg ccggtgacca gcacaccgaa ctggccgacg cgtaccagcg ccacggcctg
     1501 catcgcacgg tagttagcct ggcggaaccg ggctcggtcc gggtcacgct caccagtgtc
     1561 gatcagcgca actggcgcgg cgcggccgcc gtgcgcattc ttcgctggcc cagtcgccca
     1621 ccggacgcac cgccggacga gcgcctgctc ggctacgtgg cgctcggcaa ggccaacgcg
     1681 ttgatcgcgc gcgccgacac cgactcgtgg cgtgcttcca tccatcccat gcgccaggca
     1741 gcggcgcatt tcgcggcggc gaacgacatg caggcgctgg cggaagcgga gtatcagcgc
     1801 agcgccgtcg agctcaacct gctgttcgag ttcgacgacg gccgccggac cgcggaggcc
     1861 gcccaggcgc atttcgtggc ggcggacgat gcgacgggcg aggcccgcgc cgcggtcctg
     1921 cgtgcccgca acgaattcag cattgccgcg gccatgggcg ccgacgttcc gcgcggcgaa
     1981 cagcgtgccc tgctcgacgc cgcggtccgc cgcacgcaac aggcacaggc gtacttcgag
     2041 acgaacgatc tccacaccga cgcgatcgcc gcgttgtacg tgagctgcat ccgcgagcag
     2101 gtgctcggcg aatacgaacg ggccgcgcct gtctaccgcg ccattcgcgc gcgcgcgcgg
     2161 gaacgcggcg acaaactgtt cgaggtccgc gcaacgcaga acctggcatt catcgccgcg
     2221 cgccggggaa acgtcgcgca gtcggtggcg atgctcgatg aggtgttgcc gctgatcgat
     2281 cgagatcgca acccggacct ctacgccagg ctcatcagca atctcggctc cgggctcatc
     2341 gcgctcggcg acttcgatcg cgcgctgatg ttgcacagcg acgcgctgga gctcttttcc
     2401 gcgcgtggcg acgtgaccca gacggcgcgc gagttcgcag cgcttgcgtc gatccagttc
     2461 cgcagcggaa acgtcgagcg tgcgctggac accatcgaga gcgcgttgcc tctctacgag
     2521 cgcggcaacg accagtccga ctacatttcg gctctgcacc tcgcgggcaa cgcggcggcc
     2581 gaactcggcc ggcatgaggc cgcgctccaa tacctgcggc gggccgagca ccaggaccgg
     2641 tacggcatcg cgctcgaccg gacgcgcgtt ctgatcgccg gcgagctgcg cgcgaccggc
     2701 gacctgcgtg gcgcggaacg gctgctgtcg caggtccttc tgacccggag cgaacccacg
     2761 cgcgccgacg cactggccga gcgcgcccgc ctgcgcgaac tgcagcggcg caatcccgaa
     2821 gcgctcgccg acctgcgcga ggcggacaac atctacgcgc ggctgaagct cgacttcaat
     2881 cgcatcgaca cgagttcggc gctggccatg gcgttgctcg cggccggtga cacgcagtcg
     2941 gcgcggcgcg cggcggatac ggccgtcgcg atcgaaaccc gcatccgtgt gagctcggcg
     3001 aatcccgaac tgcgcgcccg cttcctttcg gcgagttacg cgccgtacga agcccgcatc
     3061 gaggcggaaa tcgccggtgc cgcgccgcgg gatccggcgg ccatctggca ggcgttccgc
     3121 gtcgccgagg ctatccgcgc gcgttcgttg accgaccggc ttgcgcaaac gcgaagcgtg
     3181 atcggacaac cgcgggacga agtcacggag aaattgcgcg aacgaatgac gggcctgcag
     3241 gtggaactcg aacaccggat gcgcaaggag gaagtcgacg actcggaaat ccacgagacc
     3301 cgccgtctca tcgatgaagt cagcgcgcgt ctcgagacgc gggagctgcg ccagcgcgcc
     3361 acacggtcca ccagccaact ggccatcgcc gagtcgctcg acgcggtgca ggccgcgttg
     3421 ccggcggaca ccgcggtgct ggcgtacttc gtcggcgatc ggaaatcgca tggctggttg
     3481 ttgtcgcgtt ccggtctgcg gcacgccgta ttgccgggcc ggcgagagct cggcgacctg
     3541 gtcgaagcct tcgtcatacg ccagcgcgac ggcgcgaagg cgcctgacaa ccgcgcgttc
     3601 gcgccattgt taggcaacct gctgagcgga gtgagcgccc gccggctgct ggtgctgccg
     3661 gacggtccgt tgaacggcct gccattcgcc gcgctgccgc tgccggacgg ccgcgcgcag
     3721 gacctgctca tcgatcgatt cgtcgtgagc tccgcgccct cgctggcgct cgcgcttcat
     3781 acggttgcgc gcacacaggc ggcgcaaact cgtgtcgcgg tgatctccga tccggtctac
     3841 acacccgatg accggcgcct gacggtcgcg gccaccgaaa ccgtgaacta tcgcggtggc
     3901 gagggcacta cggagcgcct cgcgcgcctg ccttattccg ccatcgaggc tcgcgccgtg
     3961 gtgcgggcgt tcaaaggcgc ggatgtcatc gagctcgcgg gattcaacgc gaccgcgcga
     4021 cgcgtgatcg agttgccatc ggaaggcctg gacgtcctgc atttcgcgac gcatgccgtc
     4081 gcccggcgcg acgtgcccgg acagtcggcg ctgtttctca gcgaattcgc cgccgacggc
     4141 tcgcggctgg cgaataaccg gctgacactg gatgacatcg cccgcagcgg tttgcgcgcc
     4201 gatgtggtcg tcctgagtgg ctgcgccacc ggcgatggcc gcgaacttcg cggcgaaggt
     4261 gtactcggcc tcacctacgg gttcctcgcc aacgggtcgc acacggtggt cgcgtcgctg
     4321 tggcccgtcg aagacgcgct gacggcgcga ttcatggagg agttctacgc gacctatcgc
     4381 gtctcgggca gcgcgccaga agcgctgcgg atcgcgcaac tgcgcgcacg gggcacgtcg
     4441 ggaccggcgg tgtggtcgag cttcgtcgtg cgggccaacg aactgccgta gcgcggcgcc
     4501 gcgcgccggc aaactaacta ccagatgagt cgagcgacac gcgtggacgc acgggttgag
     4561 gccgtctccg tcaaagcacg cgacccattt gtattaagtg tcacgacatt gcgatttctc
     4621 tcacctgcga gagcaaccag cgaaaaatat tttcgggact acttactaca aaccctgcct
     4681 atatgttccg tttccgattg gggcacaatg catgtgcctg taaacactag cgctcgccgc
     4741 gtgggattcg cttcaaatgt ccgtgcgcga tcacgtcgct acctatctcc gaaggattct
     4801 gcggttgcgc actcttgggt tgatggcaat cggtggtgtg ctttgcatca gcgcgtgcac
     4861 ggatggatcc aagaccgatg tactgcccga attttcggct gaactgaatc tatccggcga
     4921 ctccgccagt ccgactgcgc gctcgctgca ggcgggtgtg tatctcgtcg aggctcgcga
     4981 agtcgagatc gacatgcacg tcaccgtcat cgccgggggc acacgcagcg aactcgagga
     5041 tcgcattccc cgccacggcg cgatctacaa gatagttagt cttgcgtcgc cgggaactct
     5101 cgaagtgcag atccgaagcg tggatcaccg cacgaagcag ggacgcgtgc agcttcggat
     5161 cgggcgctgg aaccggccgc agcaatcgcc gccgggcgaa cgcgaactcg gctacaccgc
     5221 gttcggcgac tccgaggaac aggtggcgct cgccaccgcc gaatccgcga ctcgggccgt
     5281 cgacaaactg cacgaagcga tcacccactt cgaagccgcg gacgacgaat caatgcgcgc
     5341 gcaggcggcg ttttcgctgg cgtatctgca atccgaagga cgtgatcaat gggccgccgc
     5401 ggtgcgtgcg tcggacatcg cgaccgacgc atacgaggcc gtggacgacg aagcaggcat
     5461 tcacaacgcg gcgatcgtac gggcctcctc cgaaatcaag ctggctggcg acatgaatgc
     5521 cagcacgcag cgcgccgaac agcgcgcgat gtatgccggc gcggagcgac ggctcacgga
     5581 cgcggccacc tacttcggag ctcacggcca gcccgtgtgg gcgcaatacg cgatcaacat
     5641 gcgagccgtt ctggcggcga atctcggcga ctacgcgggc gcggggaaat tcctcgagca
     5701 gtccgtgaaa atggcgcggg acaacaatga tgtcgccgag caggcgaaat ccctcgccaa
     5761 tctggctgcc atacacgcgt acctgggctt cgtcgcgcag gcggcgcagg aatacgaggc
     5821 gctgctgccg ctgatcgatc cgcaggtgca gccgtatcag tacgcggcgt tgctcggcaa
     5881 cctgggctac acgttgatcg cactcggtga tttcgaccgc gcgctctcgt tgcgcaccca
     5941 ggcgctcgag ctctacacga aaatcggcga agaagaagaa cgcgccctgg agctggcggc
     6001 gctcggtggt ctctatttcc gcatgggcga cgccgagcgc gcgctcgaag ctttgcgctc
     6061 cgccatcgtc gaacaggaac ggatctccga cacggtcggg ctcgccagca cactgcgcgt
     6121 caccgccaac gtcgcctccg tgctcgggca acatgacacc gcgctcggat acctgcgaaa
     6181 gtccgcgctc atcgatgcga acccacacac cgtggcgcgc acgcgagtgc tgatcgcgca
     6241 ggagctgcgc gaactcggca atctcgcggc cgccgaggcg gagctcgtgt cggccctcga
     6301 gtccggcaac gcgctggtac gtgcgtcggc gctcgaagaa agagcacacc tgcgcgttgc
     6361 ccagaagaag ccggcgtcgg ccatcgacga tctgcgcgct gccgatcagc agtacgcggc
     6421 gctcggcctg gaattcaacc gcatcgatac caataccgcg ctgtcacagg cgttgttagg
     6481 cgcgcgcgac atcaagggcg cggccggcgc cgctcgcgac gcgatagcga tcgtcagccg
     6541 gatccgcacg aagtccgcga atcctgaatg gcgtgcgcac ttcctctcgg cgcggtacgc
     6601 accgtacgag gcgctcatcg cggcggaact ggcggccgcg gacgacggcg ccccgtggcg
     6661 cactttccgc atcgccgagg aagttcgtgc acgatcactc gccgacgaac tggctgccgg
     6721 cgtcattcgc caggccgatc cgcgagagga cgaacttcgt gcacgcctga ccgaccagca
     6781 attgcgcctc gagtcgcgta tgcagcggca ggacgccgac gaggccggaa cactggcgtt
     6841 gcggcattcg atcgaggaaa cgcgggctca gctcgatgcc aatcggcttc gcagtggtgg
     6901 tgtcgccgcc cgggaagctt cgattccgga ctccctgcag aaggtccaga aacagttgcc
     6961 ggcaaacacc gccgtgctcg cgtacttcgt cggcgatcgc tcgacgcacg cctggttgct
     7021 caccggccgc gagctgcgcc atgcgaccct gcccggccgc gagccgctgc agcgggccgc
     7081 cgatgcggcg gtcgccgcgc gacgttcggg ccagggagtg caggccgaac accagttgag
     7141 ttcgatgttg ctaggtcagc tgctcgatgg catttccgag actcgcatgc tggtactggc
     7201 cgacggtccg ttgaacggcg tgccgttcgc gtcgctgccc ctggcacgct ccggcaacga
     7261 aatcctggtg gatcgcttcg ttgtgggcta tgcgccatca ctggcgctcg ccatgggcaa
     7321 ctccagatcc tccaaatcga gcaacacgcg cgtcgcggtc atctccgatc cggtgtatgc
     7381 cccggacgat cgccgtctgc accttgccaa gggcggcggc aacggcaacc tccgtggact
     7441 gccgtcgacg gcgcctaaca agctcacgag gctgccgtat tcggcactag aggcgagcgc
     7501 ggtgacgagg gcatttggcg cgacggacac gattcaattg tcgggattcg acgccacggc
     7561 ggaacgcgtg ctgaagctgc cgacgcagga tcttgcggtg ctgcacttcg cgactcacgc
     7621 gcttgcgcgc caggatttgc cggatcaatc ggcgctttac ctgagcgaat acgcgaagga
     7681 tggctcggtg ctacccgcca cccgtctcac ggccagcgac atttcacggg gcgggctgcg
     7741 tgccgacgtg gtggtgctct ccggatgcgc gaccggcgac ggcagcgcgc ttcgtggcga
     7801 gggggtcctc ggacttacgt atggatttct cgccaacggc tcccattctg tcgtcgcaac
     7861 gctgtggccc atcgaggatg cctcgacggc gcgcttcatg aacgagttct atctggccta
     7921 tcgcgcatca ggccgtgcgg ccgacgcatt gcgcgccgcg cagattcgca cgcgtggcag
     7981 cgctgcgccc ggtgtgtggt cgagtttcgt ggttcgggct aacgaatttc cgtgaagccc
     8041 gcgggaagca tcaatcaaat ggctgcaatg aataatgagg agtggctcat gtacggtaaa
     8101 ttgaaaactg gtggacggat tttcgggatc tggctggcag cggcaacgct ggccctgggc
     8161 gcgtcggtcg ccgccgcgca ggaacgcgct cccgatgaag cgttgtcggg ctggctcggc
     8221 aaggaattca ccgtcgagtc ctcgacactc aatgaccaca tgccgacggg tggcaagctc
     8281 accttcgttt tcgacgccga ggacaatgtc gttcgcatct gcacgcgcaa ttcgacgcgg
     8341 cagcggaatt catggtccat ggacttcgcc acgccctgcg gcgtcacgat gttgttcacg
     8401 cgcggctcgc gttattgcac gaccgatgac gtcaagaccg gagatgccga agtgctctcc
     8461 tcgtgccatc gtctgcggtc gcgggatatc gcgatgcgtc cggcccgggt caaaggcacc
     8521 gtggaactga atgacatgat cgccttcctg gtgaaggatg gaaccggcaa gcccgtcgtg
     8581 tcgatcctgg tcgactcgcc cgcgcgtgtg acggacggcg gcatcatcct gctgaagtag
     8641 ccgagagctg gattgcgccc gcttgcgcgt cgactctcca aatcgttgct ttcgctcgcc
     8701 gcggcagcaa cgcttggcgg gtcgtgcgcg cttgcggccg cgccgaatct ctgcagtcac
     8761 gacggtagat gggcggctgc atcgacgcgc gcaccggccc cggtactcga atacctgtat
     8821 tcactcggag atgcccgttt cgacctgcgt gcggcatgcg cgcaatgggc gggcatgttt
     8881 tcccagcggc accagtccgc ggcggatagt gcacagcagt cacggcgcat tcccgcgcgc
     8941 ctgatcgtct tccccgcgga aggcgtcaac cagcgcgaac tcgtcgaagc gctcaaaaaa
     9001 accggctatg tccggatgat cgagcgctcg cgcaatttcg tggtcgtctt gctgtcgagc
     9061 ctcaccccga cggcgcggct cgtctcgatc atcaattcgc cccaggtcgg atatttcgag
     9121 cccgactgtg acggccccga ctacctgacg acctccatgg acctgccgac ggcggtgtcc
     9181 gcccagtccc attgctggca acgtgcggct cgcaagacgt ttcccaacga tccttgcctg
     9241 gatgaattgt ggggccacca gaagatcggc tgggattcgg ctgtcgccgc ccgctcgctg
     9301 ccgcgcatcg tcgccgtgct cgattccggc atcgacgcgg cgcacgagga cctggcgaga
     9361 aatgtggtcc tgaatctggc ttcaccggaa gcaccgtact cgcagggtgg acggctcaac
     9421 gcgcgttgcg ccaccagtgg ccggtgtttt ccacatggca cggagatggc cggcacgatc
     9481 ggcggcagga tggacaacgg tatcggcgtg gcgggggtgg cgcccaacag cctgctgctg
     9541 ccgatcgtca tcagccgggt cgaaagtggc ctgctggccc gaatctcgac gatcgccgcc
     9601 ggcatcgacg cggcggtcga ggatggggcg gaaataatca acataagcgc gaagtggcca
     9661 gtcgacagcc gcgccatctc ggaatccatc gtggccgcgg tcggcggcca gtcggccgtg
     9721 cggcgcctcg tcatcaccgg ctacgccacc agcctcgatc gcgacgacac ggtgcgcgag
     9781 tatttcccgt cccagtaccg ctgcctcgcc ggcgtcatcg ccgcggttcc aaccgatatg
     9841 cgtggccaag aattgttcaa tcctaaggga catccggatg cgaaagatgg ccgaattcac
     9901 gcacccggcg tggacatcgt ggtgacgacg accgagaatt ccgagcgagg ctacgccctg
     9961 tccggggccg cgggcgcatc gagcgcggcg gcctatgtct ccggggtcgt cgcgctcgtc
    10021 tggggcagcc cgccgctcaa caagtgcgac gcccagcaga taaaacaact gttgttctgc
    10081 aggagcaaga gcagcaattc cactcggtat ccatgggtca atgtcgactt ccttcgagaa
    10141 ctcgcaaacg tcgatcccgc cgtggactgt cccggcgcgc tggctgcgct cggctgcaac
    10201 taactacttg gccggccggg gtgctgaatg agcaccctgc cgctcgatga aatcgaacgc
    10261 atcgtcgccg cggccctcac cggcgaccgc gccgcggaat cccgcatgct cgtcgccctg
    10321 cggcccggcg tgctcgcggt gctgcgtttt ggcgcctttc atcgctggat agacgcggaa
    10381 gacctgaccc aggaaacgct gcacatcgtc gtcgagcgcg tgcgtgccgg gaccatcgat
    10441 gatccgcgca aggtgttcgc gttcgcggcc gccaccgcgc gcaatctcgc gctcaacgcg
    10501 gcgcgcaaga tgttgcggca gcagacggtg gtggactcgg aactcatcga cgagcttgcc
    10561 cagaacctcg aaatggagca gagcgatctg tccgattcgg acgatcgtca tctggcgcag
    10621 gctgtcgccg aacttctgga agagctgccg accgaacgcg atcgccaact gctgacccgg
    10681 ttctatctag acggtgccga taaacaattg ctgtgccgcg acctcggact ttcccctaag
    10741 catttcgatc gtgtgctgat gcgcgcccgc agccgtttgc gcactatcat cgagcgacgc
    10801 gcaccgcacc tggccctgtt tccccgaatt tcgtcaattt tgctgcccct gacgatctca
    10861 atcacatttt gggtgaggca ttttggctcc tggtgacaca agtcactcat gaagtctctt
    10921 gatcgcaact ctctcgaaga gcaaacgcgg ctggtttcgc gttatgtcgc gggcgacctg
    10981 tcccgctccg aacgcgccga gttcgaagcc tggctggtcg cgtctcccga actcgccgcc
    11041 gaagtcgaga tggaacgccg cttgcggcgc ggcatcgcga gtgcggcgcg acgcgggtgg
    11101 ctgaagcgca gcactcctgt tgaaaggatg cgcgaccgga actggcaggt cgcactggct
    11161 gccagcgttc tggtgtccgt cggcctggcg ctcagcatcc tggttccgcg cgaggccgcg
    11221 tcgccggcgg gaaacagagt cgcgtccgca agccagcgca atgcgccggc acgcactgtc
    11281 cgcctcagca gcgttcgcgg cggggccagc gcgcccgatg tcagcttctc gattgccgac
    11341 gcccccgggg ccctcacgat cgaaccggac gtggtcgtgc tgacctgcgc ggacggcgcg
    11401 atcgaactcg aatgcgcgga tggccacagc ccgcagcagc cgcaatacgc cgagtacgag
    11461 atggacctgg tgaatcgccg cggcgcgaca ctcgcgtggc gctcatcgcg ccaggcgccg
    11521 gtgggccgca cgcagctttc gttcacgctg cgtgatccgg gaagcctcga tgccggcgac
    11581 tacgatgtca tcgtgcgcgg ccattcgccc gagcacgagg aagtcgtcgc ccggttctgg
    11641 ctgcgggtcg ccccaaagta gttagccagc ccgccggcag taaaagggga cagatctatt
    11701 tttcgcgatg cgcgaaaaat agatctgtcc ccttactaac tcaaatacct tttgatgcgc
    11761 gcgtgcagcg gcgagtgccg cgggaaatgt gcgcgcagat actccatc
//
LOCUS       DATDEU010000683         2953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_132081, whole genome shotgun sequence.
ACCESSION   DATDEU010000683 DATDEU010000000
VERSION     DATDEU010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132081"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VIV63_16345"
     CDS             complement(<1..471)
                     /locus_tag="VIV63_16345"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6126224.1"
                     /translation="MNRNFRYSQLALAIAATLVSTATVAQDDMDETVVTATRTPVALD
                     AVGAPVIVISREDIERSMASDVSELLQSHAGLEISRNGGPGQTTTLFTRGTESNHTVV
                     LIDGVRINPGTIGGAALQNIAPESLERIEVVKGPRSSLYGTDAIGGVVQLFTRGA"
     regulatory      complement(574..785)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            842..1609
                     /locus_tag="VIV63_16350"
     CDS             842..1609
                     /locus_tag="VIV63_16350"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY6126225.1"
                     /translation="MSNELNAALDAARAAAEVIRGFYQRNVKIEVKADKTPVTEADVR
                     SEETIREILTKRFPAYGFYGEETGKSDMDAESVWLVDPIDGTKSFVRECPFFSTQIAL
                     MRGGRFVLGVSMAPAYDELAWAERGKGAFLNGKRISVSKVNALDAAIVSTGNLKTLAA
                     SPAWSRFGGLLGRISRIRGYGDFVHYHLLARAALDVVIESDVNILDIAALTVIVEEAG
                     GKFTDLKGAAVGLDTTTVLATNGALHDTILETVRFGS"
     gene            1621..2067
                     /locus_tag="VIV63_16355"
     CDS             1621..2067
                     /locus_tag="VIV63_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126226.1"
                     /translation="MSVFLRWGIFGILSVAGLVYAYNASKSMAEKREAQRVAAGEQAP
                     LPEAEPDAEVNPEPAADPEPAAQTAPHCQAELVVAQRALDARRRQEPLDRLLRIQEIA
                     WQEPLARRQWLEKVATRWYQLEGEEPIAEALSIAVIKDCERFSPAP"
     gene            complement(2051..2815)
                     /locus_tag="VIV63_16360"
     CDS             complement(2051..2815)
                     /locus_tag="VIV63_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6126227.1"
                     /translation="MTTLLEAEQLRVAVPGRVLVDSFDCAIRAGEFIALLGGNGAGKS
                     LLLRTLAGLRAPAAGEVRIDGIDIAKVARRDIAIRLGFLPQDPDASPQGSLSESVLLG
                     RFAHLGFWDTAGAADAQRVTRALDDVGLAPLAERELGTLSGGEQRRAAIARLLVQAPS
                     IYLLDEPTNHLDPAQQLGILDNLRQLTRAGAAVIASLHEPNLALRYADRACLMSGKGD
                     AELVDCTSLDTGHLGRLYGIGYAETRLGSQRFMAPG"
BASE COUNT          524 a          943 c         1009 g          477 t
ORIGIN      
        1 cgcaccgcgc gtgaacaact gcaccacgcc gccgatggcg tcggtgccat acaacgatga
       61 acgcggacct ttgacgactt cgatgcgctc gagcgactcg ggcgcgatgt tctgcagtgc
      121 cgccccgcca atggtgccgg gattgatgcg cacgccgtcg atgagcacca cggtgtggtt
      181 gctctcggtg ccgcgcgtga acagcgtggt agtttgtccc gggccgccat tgcgcgaaat
      241 ttccaggccg gcgtgcgatt gcagcagttc gctgacgtcg ctggccatcg aacgttcgat
      301 gtcttcgcgt gagatgacga tcaccggtgc gccgaccgcg tcgagcgcca cgggggtgcg
      361 cgtcgcggtg accacggttt cgtccatgtc gtcctgggcg accgtcgcgg ttgaaacgag
      421 agtggcagcg atggcgagcg ccagctgcga atagcggaaa ttccggttca ttgaatatgc
      481 ctttccatgc acgcccgcat ggccgaatgc ctgaacaccc aggaagcgtg cggatctgga
      541 agggcgatga gcccgtcccg ttgcccgccg caactgggaa tgatgccgtc aggccggtct
      601 ccggactctc gaactggagc attgcggctc cgacacgtcg ccttcccaag ccttgcggct
      661 cagtggcaaa tgacgtgttg cggttcgatt accgttgcgg gggcagtgct ggacttgtcg
      721 tctgtggcga ctcaccagct tcccgtaacc tttcggcggg gcgcactcta gcgatattcg
      781 gcgcgaattg acagttcaga cgcggctacc gcaggcttcg cccgccatgc cgaaccccga
      841 aatgtcgaat gaactcaatg ccgcactcga cgcggcgcgc gccgccgccg aggtgatccg
      901 cggcttctac caacgcaacg tgaagatcga ggtgaaggcg gacaagacgc cggtcaccga
      961 ggcggacgtc cgctccgaag agacgattcg cgagatcctc accaagcgat ttcccgcata
     1021 cggcttctat ggcgaggaaa ccggcaagtc cgacatggac gcggagagcg tgtggctggt
     1081 cgatcccatc gacggcacga aatcgttcgt gcgcgagtgc ccgtttttct ccacgcagat
     1141 cgcgctgatg cgcggcggcc ggttcgtgtt gggcgtgtcg atggcgccgg cttatgacga
     1201 actggcgtgg gcggagcgcg gcaagggcgc gttcctcaac ggcaaacgga tttccgtgag
     1261 caaggtgaac gcgctggatg cggcgatcgt ttccaccggc aacctgaaga cactggcggc
     1321 gtcgccggcg tggtcgcgct tcggcggatt gctcggccgc atcagccgca tccgcggata
     1381 cggagatttc gtgcactacc atctgctggc gcgcgctgcg ctcgatgtcg tcatcgaatc
     1441 ggacgtgaac atcctcgaca ttgcggcgct cacggtgata gtcgaggagg ccggcggaaa
     1501 gttcacggac ctcaagggcg cggcggtagg cctcgacacg acgacggtgt tggccaccaa
     1561 cggcgcgctg cacgacacca tcctggaaac cgtgcgcttc ggcagttagg gcggaagatc
     1621 atgtccgtat tcctgcgctg gggaatattc gggatcctga gtgtcgcggg actggtgtac
     1681 gcctacaacg cgagcaagag catggccgag aagcgcgaag cgcagcgcgt ggcggccggc
     1741 gagcaggctc ccctgcccga ggccgaacca gatgccgaag taaacccgga accggcagca
     1801 gacccggaac cggcggcgca gaccgcgcca cattgccagg cggaactcgt agtggcgcaa
     1861 cgggcactcg atgcccgccg tcgtcaggag ccgctcgatc gactgctgcg catccaggag
     1921 atcgcgtggc aggaaccgct cgcgcgacgg cagtggctcg agaaggtcgc gactcgctgg
     1981 tatcagctcg aaggcgaaga acccatcgcg gaggccctga gcatcgctgt catcaaagac
     2041 tgcgagcgtt tcagcccggc gccatgaagc gctgcgaccc cagtcgcgtt tccgcgtagc
     2101 cgattccgta gagccggccg agatggccgg tgtcgagcga tgtgcagtcg accagttccg
     2161 catcaccttt gccggacatc aggcaggccc ggtccgcgta acgcaacgcg agattgggct
     2221 cgtgcaggct ggcgatgact gcggcgcctg cacgcgtgag ctgccgcagg ttgtcgagga
     2281 tgccgagctg ttgcgcgggg tccaggtgat tggtgggctc gtcgagcagg tagatagacg
     2341 gcgcctgcac cagcaggcgg gcgatggcgg cgcggcgctg ttcgccgccc gacaaggtgc
     2401 cgagctcgcg ctccgccagc ggcgcgaggc ccacgtcgtc gagcgcgcgc gtcacgcgct
     2461 gcgcatccgc cgccccggcg gtatcccaga aacccagatg ggcgaaacgc ccgagcagca
     2521 cgctctcgct caacgaaccc tgcggggatg cgtcgggatc ctggggcagg aagcccaagc
     2581 ggatcgcgat gtcccgccgc gcgaccttcg cgatgtcgat gccatcgatg cgcacctcgc
     2641 cggcggcggg cgcgcgcagg cccgcgagag tgcgcagcag cagcgattta ccggcaccgt
     2701 tgccgcccag cagcgcgatg aattcgccgg cgcgaatcgc gcagtcgaaa gagtcgacga
     2761 gcacccggcc cgggacggcg acgcgcagct gctcggcttc gagcagggtt gtcatgggcg
     2821 cgtccagcga atctcgagcg cggtggcggc gcccaccacg acctggtcca gcgactccgg
     2881 catctcgaag gtccgcgcgt cgacggcaaa acgcccggcc atgatttccg cgaacgcctg
     2941 cggataccgg gga
//
LOCUS       DATDEU010000684         5648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2028832, whole genome shotgun sequence.
ACCESSION   DATDEU010000684 DATDEU010000000
VERSION     DATDEU010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028832"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..732
                     /locus_tag="VIV63_16365"
     CDS             127..732
                     /locus_tag="VIV63_16365"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6126228.1"
                     /translation="MALKSVESQPPRALGASPDASERELLERIARSKDQAAFRTLYGN
                     YYQRLSRLLARMSVRREDIEEVINDTFWVVWTKAGEFRGASQLSTWIIGIAYRRALNA
                     LRRAKIRPVSDEPFDEDSISVASTDAAETDGQWLALGLSRLPVEQRMALELTYTLGHS
                     CEEVAAILDCPVNTVKTRLFRARETLKLVLPGLAGMTGENS"
     gene            729..1412
                     /locus_tag="VIV63_16370"
     CDS             729..1412
                     /locus_tag="VIV63_16370"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY6126229.1"
                     /translation="MSPRIPDTHAEAWALLPWLANGRLQASDREWVEAHVAQCAECRE
                     ELAVQRKAATEMSRQMDQDSLPEAAATDEHRSFNKLWARIEASESVSLPAREAGGAAR
                     ATRSSRTVRWLAAAVVVQGFGLALFGFNALRGDQPAGGYVTVTDAPSQRLNAPAVRIV
                     FAPDASVANINTMLTHQGLSIVSGPGTSGNFTAELSADAVASGASADSVAAVIAKDPN
                     VTFAQPVAR"
     gene            1381..2694
                     /locus_tag="VIV63_16375"
     CDS             1381..2694
                     /locus_tag="VIV63_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY6126230.1"
                     /translation="MSRSHNRLRVDSRANWRLAIALTLAAAFALSRAATAAPPPVLTP
                     PEVRTAPAQFIVVAVANPVTGRPGAVGGTAHGYGTTTNYRVSASATATIRDIASQYSL
                     TRVSEWPIEQLNMHCVLFRIPPGTTREAMIEKLKADKRVLIVQPLNEFESAATAGFDD
                     PYARLQSNVSALDVAEAHNISRGAGIRVAIIDTGVDTRHPDLAGRTQLTRNYIDADEA
                     AFRGDRHGTQVAGLIAAAANNGVGIVGVAPDVRLLAFKACWQVSATTAGRCNSFTIAQ
                     ALADAIAARAHVINLSLVGPSDPLLEALIAKAVGAGVIVVGAVSTDPRFGFPASLPHV
                     LPVAEAEISDGVGDDILRAPARDIVTLVPDGHYDFASGSSLATAQISGVVALLLARNQ
                     KLGITRMRELLTQSTQNHDTTRGPFRSVNACTALAQVVRGARCGG"
     gene            complement(2989..4134)
                     /locus_tag="VIV63_16380"
     CDS             complement(2989..4134)
                     /locus_tag="VIV63_16380"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEY6126231.1"
                     /translation="MATTGNVEPAFSLGLEEEYLLVDLATRDLVSDPPAELLQRCIAE
                     TDGRVSPEFLRSQLEVKTSVCVSVASMRRELAELRRKVCEVAADYGIAPIAASTHPFA
                     RPVQRPPHTDHERYADIAREVQGGARRMIICGMHVHVGLDDDELRIDVMNQLSYFVPH
                     LLALSCSSPFWEGEDMGMQSFRLMLLSSLPRTGLPEHFESYGEFRRHLDALVRNGLIE
                     DTTKIWWDIRPSSRYPTLETRVFDCCTLLDDAVCLAALNLSLVRMLYRLRRENKVWRQ
                     YPRMLIAENRWRAMRFGSDATLLDLARGELVAFPELLEELLAITRDDAIALDCLAETE
                     HARVLIKRGTSAHQQMDVYQTARSQGATDSEALSAVVGWLAHKTCAF"
     gene            complement(4168..>5648)
                     /locus_tag="VIV63_16385"
     CDS             complement(4168..>5648)
                     /locus_tag="VIV63_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6126232.1"
                     /translation="TLDRVLIAGETSVPSDHIVLVNRRGEELAIQETATPIRNREGAA
                     IGAVIVFGDVTKERRLKRALSYQASHDALTGLINRREFDTRLETAVTAAQRGEGEFVL
                     LYVDLDQFKVVNDTCGHSAGDRLLRDITSLLQTRVRASDTIARLGGDEFGLLLERCSL
                     EQAERVADSIRQAIHGYRFLWGANSLSVGASIGVVRIVRETTSAASVLSAADIACYAA
                     KDGGRNRVQIYERDHGTNRHREMQWVGRIARAVEDGRLELYAQRIVGISAKTTDMPFF
                     ELMVRLRDEDGTLVPPSEFIPAAERYNVMVMVDRWVVGRAIELLETCARQGRRLPLVA
                     VNLSGTSINDEDFLEYILSRLNDDQVAHALCFEITETAAVASLSKATYFMRELKARGC
                     RFSLDDFGSGVSSFVYLKTLPVDFLKIDGHFVAHVATDVVDRSMVEAIAKIGSAMEVA
                     TIAEKVESAEVLQVLKQIGVDYIQGVHLAEPCAIDEVFSCGN"
BASE COUNT          952 a         1871 c         1855 g          970 t
ORIGIN      
        1 gatatggttg gcgaccgctt cgccgtgacc gccctatggc gcttttcgag cactcaatag
       61 cctcgcggga accgtactcc cggtccgcgt tactcatggg cagtacgtcg atgaaggtgg
      121 actgccgtgg cactaaagag cgttgaatcc cagccgccgc gggcacttgg cgcaagtccc
      181 gacgcctctg aacgcgagct gctcgaacgc atcgcccggt cgaaggacca ggcggctttc
      241 cgcacgttgt atggcaacta ctaccagcgc ctctcgcgcc tgctggcccg catgtcggtg
      301 cgccgcgagg acatcgagga agtcatcaat gacacgttct gggtggtctg gaccaaggcg
      361 ggggaattcc gcggggcctc gcagctttcc acctggatca tcggaatcgc ctaccggcgc
      421 gcgctcaatg cactgcgccg cgccaagatc cggcccgtat ccgacgaacc gttcgacgag
      481 gactccatca gcgtagcctc cacggatgcc gccgagaccg atggccagtg gctcgccctg
      541 gggctgtcgc ggctgccggt cgaacaacgc atggcgctcg aactcaccta tacgctcggc
      601 cattcctgcg aagaagtggc cgcgatcctc gattgcccgg tcaacaccgt gaagacacgg
      661 ctgttccggg cgcgcgaaac tctaaaacta gtgttgcccg gcctcgccgg catgaccgga
      721 gaaaattcat gagtccccgc attcccgata ctcacgctga agcctgggcg ctgttgccgt
      781 ggctcgccaa cggccgcctt caggcgtccg atcgtgaatg ggtggaagcg cacgtagcgc
      841 agtgcgccga atgccgcgag gaactggccg tgcagcgcaa ggctgcgacg gagatgagcc
      901 gccagatgga ccaggactcc ctgcccgaag ccgccgccac cgacgaacat cgctcgttca
      961 acaagctgtg ggcgcgcatc gaggcttccg aatccgtttc gctgccggcg cgcgaagcag
     1021 gtggtgcggc gcgtgccacg cgctcgtcgc gcaccgttcg ctggctcgcg gccgcggtgg
     1081 tcgtgcaggg tttcggactt gcgttgttcg gcttcaatgc actgcgtggc gatcagccgg
     1141 ccggcggcta cgtcaccgtc acggatgctc cgtcgcagcg gctcaacgcg ccggccgtgc
     1201 gcatcgtgtt cgcgccggac gcatccgtgg ccaacatcaa tacaatgctc acgcaccagg
     1261 ggctgtcgat cgtctcgggc ccgggcacgt cgggaaattt caccgcggaa ctgtcggcag
     1321 acgccgtggc ctccggcgcc tcggcggatt cggtcgccgc cgtgatcgcg aaggatccga
     1381 atgtcacgtt cgcacaaccg gttgcgcgtt gattcccggg ctaactggcg gctggccatc
     1441 gcgctgacgc tggccgccgc gttcgcgctg tcgcgcgccg ccacggccgc accgccgccg
     1501 gtgctcacgc cgcccgaagt ccgcaccgcc ccggcccagt tcatcgtcgt ggcggtcgcc
     1561 aatccggtca ccggcaggcc gggcgcggtc ggcggcactg cccacggtta cggtaccacc
     1621 actaactacc gtgtgagcgc gtcggcgacg gcgaccatcc gcgatatcgc cagccagtat
     1681 tcgctgacgc gcgtgtccga atggccgatc gaacagctca acatgcactg cgtgttgttc
     1741 cggatcccgc ccggcacgac tcgcgaagcg atgatcgaaa agctgaaggc ggacaagcgc
     1801 gtcctcatcg tgcagccgct caatgaattc gagtccgcgg ccaccgccgg gttcgacgac
     1861 ccgtacgcac ggctgcagtc gaacgtcagc gcgctggatg tcgccgaagc gcacaacatc
     1921 agccgcggcg ccggtatccg tgtcgccatc atcgacaccg gcgtcgacac gcgccatccc
     1981 gacctcgcgg gccgcacgca gctcacgcgc aactacatcg atgccgacga ggcggcgttt
     2041 cgcggcgatc ggcacggcac gcaggtcgcg ggactcatcg cggccgcggc caacaacggc
     2101 gtgggtatcg tcggcgtggc acccgacgtg cggttgctgg cgttcaaggc ttgctggcag
     2161 gtgtcggcca cgacggccgg ccgctgcaat tcgttcacca tcgcccaggc gctcgcggat
     2221 gccatcgccg cccgcgcgca cgtcatcaac ctgagcctgg tcggcccgag cgatccgctg
     2281 ctcgaggcgc tgatcgccaa ggccgtcggc gccggtgtga tcgtggtggg cgccgtgtcg
     2341 acggatccgc gtttcggctt tcccgcgagc ctgccgcacg tgctgccggt ggccgaagcg
     2401 gaaatttccg acggcgtcgg cgacgacatc ctgcgcgcac cggcgcgcga catcgtcacg
     2461 ctggtgccgg acggtcacta tgactttgcg tcgggcagct cgctcgccac cgcacagatt
     2521 tcaggagtcg tcgccctgct gctggcgcgc aatcagaagc tgggcatcac gcgcatgcgc
     2581 gaattgctga cgcagtccac gcagaatcac gacacgacgc gcgggccgtt ccggtcggtg
     2641 aatgcgtgta cggcacttgc gcaggttgtg aggggcgcga ggtgtggggg gtagcggagg
     2701 agagttggat gatagaaggg ccccagcgat cgccatcaaa ccctgctccg attctccgct
     2761 gcggtgagaa gagaagagtc gctagcagcc aggtccttcc acagccgggg cctcgcaatg
     2821 ctacgaccga atgtcttcgc gtcaggcggc ggtcccaatc ttgcgatgcc aacttcctga
     2881 acgaagggcc cggcgaaaag cgaggctcat cacccgcagg ggtcgcagtc gctcgccctt
     2941 ctccgatcag ccccgtcccc ttttgctagc aaatcaggaa tgtccccatt aaaaggcgca
     3001 ggtcttgtgc gccagccagc ccaccactgc tgacaacgcc tcgctgtcgg tcgcgccctg
     3061 tgagcgggcg gtctggtaca catccatttg ctggtgcgcg ctggtgccgc gcttgatcaa
     3121 tacgcgcgcg tgttcggtct ccgccaggca gtcgagtgcg atcgcgtcgt cacgcgtgat
     3181 tgccaggagc tcttcgagca gctcggggaa cgcgaccagc tcaccgcgtg ccaggtcgag
     3241 cagcgtggcg tcgctgccga agcgcatggc gcgccagcgg ttctcggcga tcagcatgcg
     3301 cgggtattgc cgccagacct tgttctcgcg gcgcagccgg taaagcatgc gtaccagcga
     3361 gagattgagc gccgccaggc acacggcgtc gtcgagcagc gtgcagcaat cgaacacgcg
     3421 ggtctcgagc gtcggataac gcgaactcgg gcggatgtcc caccagatct tggtggtgtc
     3481 ttcgatgagt ccgttgcgca ccagtgcgtc gaggtggcgg cggaactcac cgtagctctc
     3541 gaagtgttca ggcaagccgg tgcgcggcag cgatgacaac agcatgagcc ggaacgactg
     3601 catgcccatg tcttcgcctt cccagaacgg cgaggaacag gacagcgcca gcaggtgcgg
     3661 cacgaagtag ctgagctggt tcatcacgtc gatgcgcagt tcgtcgtcgt cgagcccgac
     3721 gtgcacgtgc atgccgcaga tgatcatgcg gcgcgccccg ccctgcactt cgcgcgcgat
     3781 atccgcataa cgctcgtggt cggtatgcgg cgggcgctgc acgggccgcg cgaacgggtg
     3841 ggtggatgcg gcgatgggcg cgatgccgta gtccgccgcg acttcgcaca ccttgcggcg
     3901 cagctcggcg agctcgcgcc gcatggaagc gaccgagaca cacacggacg tcttcacttc
     3961 gagctgggat cgcaggaatt ccggactgac gcggccatcg gtctcggcga tacatcgctg
     4021 caggagttcg gcgggagggt cggaaacgag atcgcgcgtg gcgagatcca cgagcagata
     4081 ttcttcttcc agccctaacg aaaaggctgg ttcaacgttc cccgtcgttg ccatgagcgg
     4141 caacactaca cgggttagtg cggcgcgtca gttcccgcag gagaagactt catcgatggc
     4201 gcagggctcg gccagatgga ctccctggat gtagtcgaca ccgatctgct tgaggacctg
     4261 cagcacctcg gcgctctcca ccttctccgc gatcgtcgcc acctccatgg cgctgccgat
     4321 cttggcgatg gcctcgacca tgctgcgatc gacgacgtcg gtggccacat gcgcgacgaa
     4381 atggccgtcg atcttcagga aatccacggg cagggtcttg agatacacga atgacgacac
     4441 gccgctgccg aaatcgtcga gcgagaaacg gcagccgcgc gccttcagct cgcgcatgaa
     4501 atacgtcgcc ttggaaaggc tcgcgactgc ggcggtttcg gtgatctcga aacacaacgc
     4561 gtgggccacc tggtcatcgt tcaatcgcga caggatgtac tcgaggaagt cctcgtcgtt
     4621 gatggacgtg cccgacaggt tgacggccac cagcggcagg cgccgtcctt gcctcgcgca
     4681 ggtttccagc aactcgatgg cgcggccgac cacccagcgg tcgaccatga ccatcacgtt
     4741 gtaacgttcg gccgcgggaa tgaactcgct gggcggaacc aaggtgccgt cttcgtcgcg
     4801 caggcgcacc atgagctcga agaacggcat gtccgtggtc ttcgccgaaa tgccgacgat
     4861 gcgctgggca tagagctcga ggcgcccgtc ttcgaccgct cgcgcgatgc gcccgaccca
     4921 ctgcatttcg cgatggcggt tggtgccgtg gtcgcgttcg tagatctgca cgcggttgcg
     4981 gccgccgtcc ttggccgcgt agcacgcgat gtccgccgcc gacagcaccg atgcggcgct
     5041 cgtggtttca cgcacgatgc gcacgacgcc gatgctggcg ccgaccgaca ggctgttcgc
     5101 gccccagagg aatcgatagc cgtggatcgc ctggcggatg ctgtcggcca cgcgctccgc
     5161 ctgctcgagt gagcagcgtt cgagcagcag gccgaattca tcgcctccca ggcgagcgat
     5221 ggtgtccgac gcgcgcacgc gcgtctgcag gaggctggtg atgtcgcgca gcagccggtc
     5281 gccggcgcta tgtccgcagg tgtcgttcac caccttgaac tggtcgaggt cgacgtacag
     5341 cagtacgaat tcgccctcgc cgcgttgcgc ggcggtcacc gcggtttcga gccgcgtatc
     5401 gaactcgcgg cggttgatga ggccggtgag cgcgtcgtgg ctcgcctggt aggacagcgc
     5461 gcgcttcagg cgccgttcct tggtgacgtc gccaaacacg atgacggcgc cgatcgctgc
     5521 gccttcgcgg ttgcggatcg gcgtcgcggt ttcctggatc gccagttcct cgccgcggcg
     5581 gttcacgagc acgatgtgat cggacggcac ggacgtctcg ccggcgatga gcacgcggtc
     5641 gagcgtat
//
LOCUS       DATDEU010000685         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_572613, whole genome shotgun sequence.
ACCESSION   DATDEU010000685 DATDEU010000000
VERSION     DATDEU010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572613"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VIV63_16390"
     CDS             <1..490
                     /locus_tag="VIV63_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126233.1"
                     /translation="DDLVDALPKVPLGLDETDENRKHRAEAYALATACTLAGREMSSI
                     PFGGGRIGSRIDEAVKLEPKNPRVRLVEALAMFERAGKNADEKAAALRNLRNVTVMFE
                     QARAAAASTPEWGAAEAYAFLGRALYDMRDVVGAREALERSLLFAPDYAFARQLMSRI
                     TR"
     gene            519..>2089
                     /locus_tag="VIV63_16395"
     CDS             519..>2089
                     /locus_tag="VIV63_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEY6126234.1"
                     /translation="MKYGRLFAPLDLGFTRLPNRIVMGSMHTGLEDRARDFPKLAAYF
                     AARARGGAGLLVSGGIAPNRAAWAKPFAGKLSTQREVARHRLVTDAVHTEGGKICMQI
                     LHTGRYGYHPFLVAPSALRAPINRFKPHALSSDGVRDQIADFVHCAVLAQQANYDGVE
                     IMGSEGYFINEFLAPRTNNRTDEWGGSLENRARLALEVVRQTRAAVGRDFIIIFRISG
                     LDLVEGGGTGAEVEWLASQMEGAGATMLNTGIGWHEARIPTIAGVVPRGAFGWVSARI
                     KAATRLPVIATNRLNAPEDAESLLAGGSADLVSMARPLLADPDFPRKASEGRENEINT
                     CIACNQGCLDKVFENQRATCLVNPLAAYETEIRIESTRAIKRVAVVGAGPAGLACATT
                     LAERGHRVTLFERAHEIGGQFRYAREVPGKEDFRDTLRYFARRIELTHVELRLNTVAD
                     AIGLAADGFDAFVISSGVAARVPQIPGIDHAKVISYPELLSGARRAGESVAIIGAGGI
                     GFDVATYLTHSHGSD"
BASE COUNT          348 a          696 c          708 g          337 t
ORIGIN      
        1 agacgacctg gtcgacgcgt tgccgaaggt gccgctcggt ctcgacgaaa ccgacgagaa
       61 ccggaaacac cgcgcggagg cctatgcgct tgccacggcc tgcacactcg ccgggcgcga
      121 gatgagctcc attcccttcg ggggcggacg catcggttcg cgcatcgacg aggccgtaaa
      181 gctcgagccg aagaatccgc gcgttcggct ggtggaagcg ctggccatgt tcgaacgggc
      241 cggcaagaac gccgacgaga aggcggccgc gctccggaat ctgcgcaacg tcacggtcat
      301 gttcgagcag gcgcgcgccg ccgcggcgag cacgccggag tggggcgccg ccgaggccta
      361 tgcgtttctc ggccgcgcgc tctacgacat gcgcgacgtg gtgggtgcac gcgaagcact
      421 cgaacgctcg ctgctgttcg cgccggacta cgcgtttgcg cgccagctga tgtcgcgaat
      481 cacgcgttag cccgccaaca ccgctacaat cggcgcccat gaaatacggg cgcttgtttg
      541 ccccgctcga tctgggcttc acgcgcctgc cgaaccgcat cgtcatgggt tcgatgcaca
      601 ccgggctgga agatcgcgcg cgcgatttcc cgaagctcgc cgcgtatttc gctgcccggg
      661 cgcgcggtgg cgcggggctc ctggtgagcg gtggcatcgc accgaatcgc gccgcctggg
      721 ccaaaccctt cgccggcaaa ctttccacgc aacgcgaagt cgcccggcat cgcctcgtga
      781 ccgacgcggt gcacaccgag ggcggcaaga tctgcatgca gatcctgcac accgggcgct
      841 acggctatca cccgtttctc gtggcgccga gcgccctgcg ggcgccgatc aatcgcttca
      901 agccacacgc gttgtcatcg gatggcgtgc gcgaccagat cgccgacttc gtccattgcg
      961 ccgtgctggc acagcaggcg aactacgacg gcgtcgagat catgggttcg gaggggtact
     1021 tcatcaacga gttcctcgcg ccgcgaacca acaatcgcac cgacgaatgg ggcggctcgc
     1081 tcgagaatcg ggcgcggctc gcgctcgagg tggtgcggca gacgcgggcg gccgtgggcc
     1141 gggacttcat catcatcttt cgcatctcgg gtctcgacct cgtggaaggc ggcggtacgg
     1201 gcgccgaagt cgagtggctc gcttcgcaga tggaaggcgc tggcgccacg atgttgaata
     1261 cgggcatagg ctggcacgag gcgcgcattc ccaccattgc cggcgtggtg ccgcgcggcg
     1321 cattcggctg ggtgtcggcg cgcatcaagg cggccacgcg gcttcccgtc atcgccacca
     1381 atcgcctgaa cgctcccgag gatgccgagt cgctgctcgc cggcggaagc gcggacctgg
     1441 tgtcgatggc gcggcccttg ctcgccgacc ccgacttccc gcgcaaggcg tcggaaggac
     1501 gcgagaacga aatcaacacg tgcatcgcct gcaaccaggg ttgtctcgac aaggtgttcg
     1561 agaaccagcg ggcgacctgt ctcgtgaacc cgctggcggc ctacgagacg gagattcgca
     1621 tcgagagtac gcgcgccatc aagcgcgttg ccgtcgtggg cgccgggccc gcgggacttg
     1681 cctgcgccac gacgctggcc gagcgcggcc atcgcgtgac gctgttcgag cgcgcgcacg
     1741 aaatcggcgg acagtttcga tatgcgcgcg aagtacccgg caaggaggat ttccgcgaca
     1801 cgctgcgtta cttcgcgcgg cgcatcgaac tcacgcacgt ggagctgaga ctcaacacgg
     1861 tcgcggatgc gatcggcctc gctgccgacg gcttcgacgc tttcgtcatc tcgagcggcg
     1921 tcgccgcgcg cgtgccgcag atcccgggca tcgatcatgc gaaggtcatc tcctatccgg
     1981 aactgctttc cggagcgcgc cgggcgggtg aatcggtggc gatcatcggg gccggcggca
     2041 tcggtttcga cgtggcgacc tatctcacgc acagccatgg cagtgacta
//
LOCUS       DATDEU010000686         6498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_717262, whole genome shotgun sequence.
ACCESSION   DATDEU010000686 DATDEU010000000
VERSION     DATDEU010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717262"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VIV63_16400"
     CDS             complement(<1..210)
                     /locus_tag="VIV63_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY6126235.1"
                     /translation="MFKKILIANRGAIACRILRTVKALGLRSVAVYSEADENSPHVRM
                     ADEAFCIGPAPAAESYLRAERILEVA"
     gene            complement(284..907)
                     /locus_tag="VIV63_16405"
     CDS             complement(284..907)
                     /locus_tag="VIV63_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HEY6126236.1"
                     /translation="MNGLDEVVPAGEPWMGVVRLGQSLRIVDLEGNQAVDTLFYNAAD
                     TAEHYSAQNTIQSQGGIYLTTGTVLLSNRGNPLLRIEEDTCGRHDTLGGACSAESNTV
                     RYALEKRPMHSCRDSFLLALANHPRGKTGELTKRDLPSNINFFMNVPVTPAGELRFAD
                     GISAAGRHVQLRALVDTLVLISNCPQLNNPCNAYNPTPIRVMTWDNA"
     gene            complement(904..1608)
                     /locus_tag="VIV63_16410"
     CDS             complement(904..1608)
                     /locus_tag="VIV63_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HEY6126237.1"
                     /translation="MLWEEEIPGGSHWSGVIRRGVVLRLTAGGNDANVAALFYSREQT
                     LERYNMADTLKAQHIAHLTTGCVCYSDMGRILCSITADTCGWHDPLCGLTDAALIAAR
                     YGATRFQEQRNQRYVSGRDGMLVELGKYGLNERDLVANINFFSKVVVDEAGTLKLAAG
                     HSRAGQYVDLRFEMDTIVALHAGPHPLATAPKYAPPAIKLTARRGEPPAADDPCRLRC
                     PENARGYINTERMYSS"
     regulatory      1811..1915
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            1947..3038
                     /locus_tag="VIV63_16415"
     CDS             1947..3038
                     /locus_tag="VIV63_16415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6126238.1"
                     /translation="MPRIQPSLRRLLQTFFLAVFVGAALLVAQPARSAEKTSFNVAWS
                     IYVGWMPWDYAQQSGILKKWADKYGITIKLTQMDYIESINQYTAGKFDGCVMTNMDML
                     TIPSAGGVDSTALIVGDYSNGNDGVVLKGAGKKLADIKGQSVNLVELSVSHYLLARGL
                     ESVGLAERDIKVVNTSDADIVSAFATPGVTATVTWKPQLSEVTAMPKTTLVFDSSKTP
                     GEILDLMVVNSAVLKANPKLGKALVGAWYETLSVMLAKDAKATAALESMAKASGTDLA
                     GFKGQLATTFLYSTPATAGAATKSPDLVKKMDLVRKFSFDHGLLGEGAKTVDAVGIEF
                     PGGVTLGNKANLKLRFDPAYMQMAADGKL"
     gene            3067..3873
                     /locus_tag="VIV63_16420"
     CDS             3067..3873
                     /locus_tag="VIV63_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6126239.1"
                     /translation="MINQHPGRAAGLALGVLPFIALLVMYVIASHLRLAENPDDKLLP
                     ALGKIASTFHTYAFEEDTRTGRVLFWADTWASLKRIGSALLISATLGVVLAVGIGVIP
                     RLRALLSPLLGTLAMIPPLAILPVLFIVLGLEETAKISLIVIGIAPCIARDLALRVGE
                     LPKEQIIKAQTLGASTWQLILRVVLPQLWPRLIDSLRLSMGSAWLFLIAAEAIASTEG
                     LGYRIFLVRRYLAMDVILPYVVWITLLAVATDLGLRVLRARAFRWAEPER"
     gene            3873..4610
                     /locus_tag="VIV63_16425"
     CDS             3873..4610
                     /locus_tag="VIV63_16425"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6126240.1"
                     /translation="MATVTVRNVWKEYGAQVVLENVRLEIADHEFVTIVGASGCGKTT
                     FLKMLLGMEQPTRGQILIDGEPIRPEPDPDRGVVFQRYSSFPHLTVVENVMLGLELRS
                     SRLAGKLFGAARREARDAALAMIEQVGLTHARDRYPAQLSGGMQQRMSIAQAVIRKPK
                     ILLLDEPFGALDPGVTADMHELILRLWEENRMTIFMVSHDIQESFQLGTRLLTFDKLR
                     HDPQSPEAYGASVTYDLPLTRAKAASP"
     gene            4777..6024
                     /locus_tag="VIV63_16430"
     CDS             4777..6024
                     /locus_tag="VIV63_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126241.1"
                     /translation="MQTAAGSPFSTATEMELASRFLEATDAPRLQHAISALIKRARDA
                     SGRKLDAATARRLRQQLLDTATRLGFPQIGRLRHAAIRPPQVARGMGVLSRAARSLGV
                     ELEGLSPEDKEFEIARRFVRLGGQTARRLRREPASPSCACQGSCGCSSCGGAPADIPD
                     TSNRSHAMHDIDHTKSEYDPELDELDELETDEMEFSDEFDSGELDEMETDAGEYDSGE
                     LDELELDETSGPFSEVEEAELAQELLGLSSEAELDQFLGKIFKKAWRGVRGLGKTLGR
                     VARPLGGILKGVAKKLLPVAAGAAGTFFGGPAGAAIGSKIGSVVGNALEAEFEGSPED
                     REFEAAKTFVRLAGNAAQQAANTSPGAPPVSTAKNAVVSATRNSVQRLRRRRSRGASN
                     YHGGSRRTGRWVRRGNRIILFGV"
     gene            6031..>6498
                     /locus_tag="VIV63_16435"
     CDS             6031..>6498
                     /locus_tag="VIV63_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126242.1"
                     /translation="MTPDAHRLLTQEARALLTRLALVKPLVLQETLVPAANVSDTAAA
                     GIERALASGRRTVRTLVNGFLGWLRGEGGAHATDAQAQRRFSLVRLQFNTMLAQFDIF
                     ADAYSQRSEHDIGVWLAGLDVAAADALALPRYYRSPALVCYLDRGIGAAIRRAR"
BASE COUNT         1110 a         2136 c         2129 g         1123 t
ORIGIN      
        1 agcgacttct aggatgcgct cggcgcgcag gtaactttcc gcggccggcg ccggtccgat
       61 gcagaaggct tcatcggcca tccgcacgtg cggcgagttc tcgtcggctt cggagtagac
      121 cgcgaccgag cgaagtccca gggccttcac ggtgcgcagg atgcggcacg cgatggcgcc
      181 gcgattggcg atcagaattt tcttgaacat ggcgcaattc cccgggtcgt cccggtgttg
      241 gaggaattgc gcgacgggtc gtcccgccgg tgggagaacg cgctcatgcg ttatcccagg
      301 tcatgactcg aatcggagtc ggattgtagg cgttgcaggg gttgttgagc tgagggcagt
      361 tcgaaatcag caccagcgtg tcgacgagcg cccgcaactg cacatgccgg cccgccgcgg
      421 agatgccgtc ggcgaaccgc agttcacccg cgggcgtcac cggcacgttc atgaagaaat
      481 tgatgttgga gggcaggtcg cgcttggtca gctcgccggt ttttccccgc gggtgattcg
      541 ccagcgccag caggaagctg tcgcgacagg aatgcatcgg ccgtttctcg agcgcgtaac
      601 gcacggtgtt gctctcggcc gagcaggcgc cgcccagcgt gtcgtgccgg ccgcaggtgt
      661 cttcttctat gcgcaacaaa gggttgccgc ggttcgagag cagcacggtg cccgtggtga
      721 gatagatgcc gccctgcgac tggatggtgt tctgggcgct gtaatgctcg gcggtgtcgg
      781 cggcgttgta gaacaacgtg tccaccgcct ggttgccttc gaggtcgacg atgcgcagcg
      841 actgtccgag ccgcacgacg cccatccacg gctcacccgc gggcaccacc tcatcaagac
      901 cgttcaagat gaatacatcc tttcggtgtt gatgtagccg cgcgcgttct ccgggcagcg
      961 aagccggcag ggatcgtcgg ctgccggcgg ttcgccgcgt cgcgccgtca gctttatggc
     1021 cggcggcgcg tatttcgggg ccgtggccag cggatggggt cccgcatgca gggccacgat
     1081 ggtgtccatt tcgaatcgca ggtcgacgta ttgaccggcc cgggagtgac ctgcggcgag
     1141 tttcaatgtg ccggcctcat ccaccactac cttgctgaaa aagttgatgt ttgcgacgag
     1201 gtcgcgctcg ttgaggccgt acttgccgag ctccaccagc atgccgtcgc gcccgctcac
     1261 gtagcgctga ttacgctgct cctggaatcg cgtggcgccg tagcgcgcgg cgatcagcgc
     1321 ggcatccgtg agcccgcaca gcggatcgtg ccagccgcag gtgtcggcgg tgatggagca
     1381 gaggatccgc cccatgtccg aataacagac gcagcccgtc gtcaggtgcg cgatgtgttg
     1441 cgccttgagc gtgtcggcca tgttgtaacg ctcgagcgtc tgctcgcggc tgtagaacag
     1501 cgccgcgacg ttggcgtcgt tgccaccggc cgtcagccgc aatacgacgc cgcgccggat
     1561 gacgcccgac cagtgagacc cgcccggaat ttcttcttcc cacaacatgc acagcccagg
     1621 tgcaaagcct gtaccacgca atgatctcca ggcgccggtt gctggagccg atgcgcgcga
     1681 agtttgctcc gcgagcgtgc acgcatcgac gcgcgcgcgt ttgtttggtg cagctttgcc
     1741 gtccgatgac gctaacaact cgaacggcgc tgcgggcgac ctcggcacag ctcttgcaaa
     1801 aacccacttc agaatgtgcg cctagggttc cggctgaagg atcagcgctg gtccgagaga
     1861 cgcagtccgg cgattcacgt caccggatac acggagggac aaaagcccgg gagatgcctg
     1921 atgaactaac aacacgggag actctcatgc ccagaattca gccttctctc cgccgccttt
     1981 tgcagacctt tttcctcgcc gtcttcgtcg gcgcagcgct cctcgtcgcc cagcccgcgc
     2041 gctcggccga aaaaacctcg ttcaacgtcg cctggagcat ctacgtcggc tggatgccct
     2101 gggactacgc gcaacagagc ggcatcctca agaagtgggc cgataaatac ggcatcacca
     2161 tcaaactcac gcagatggac tacatcgagt ccatcaacca gtacacggcc ggcaagttcg
     2221 acggctgcgt catgacgaac atggacatgc tgacgatccc ctcggccggt ggcgtcgatt
     2281 ccacggcgct gatcgtcggt gactactcga acggcaacga cggcgtggtg ctcaagggcg
     2341 ccggcaagaa gctggctgac atcaagggtc agtcggtgaa tctcgtcgag ttgtcggtgt
     2401 cgcattacct gctggcccgc ggcctcgaat ccgtcgggct cgccgaacgc gacatcaagg
     2461 tcgtgaacac ctcggacgcg gacatcgtca gcgcgttcgc cacgccgggc gtcacggcga
     2521 ccgtgacctg gaagccgcaa ttgtcggaag tcacggcgat gccgaagacc acgctggtgt
     2581 tcgactccag caagacgccc ggcgaaatcc tcgatctcat ggtggtcaac agcgcggtgc
     2641 tgaaggccaa cccgaagctc ggcaaggcgc tggtcggtgc gtggtacgaa acgctgtcgg
     2701 tgatgttggc gaaggacgcc aaggctacgg ccgcgctcga atcgatggcc aaggcctcgg
     2761 gcaccgatct cgccggcttc aagggccagc tcgccaccac gttcctgtat tcgacgccgg
     2821 ccaccgcggg ggccgccacc aagtcgcccg acctcgtgaa gaagatggac ctggtccgca
     2881 agttctcgtt cgaccacggc ttgttaggcg aaggcgccaa gaccgtcgac gcggtgggca
     2941 tcgaattccc gggcggcgtc acgctcggca acaaggccaa tctgaagctg cgcttcgatc
     3001 cggcgtacat gcagatggcg gcggacggaa agctctgaag cgcccacgcg tttcctcgtg
     3061 cgccggttga tcaaccaaca cccggggcgg gccgctgggc tcgccctggg tgtgttgcca
     3121 ttcatcgcgc tgctggtgat gtacgtcatc gcgtcgcacc tgcggctcgc ggaaaacccc
     3181 gacgacaaac tgctgccggc gctcggcaag atcgccagca ctttccacac ctacgcgttc
     3241 gaagaggaca cacgcacggg ccgcgtgttg ttctgggccg atacgtgggc gagtctcaag
     3301 cgcatcggtt cggcgctgct gatcagcgcc acgctcggcg tcgtcctggc agtcggcatc
     3361 ggcgtgatcc cccggctgcg cgcgttgctc tcgccgctgc tcggcacgct ggcgatgatt
     3421 ccgccgctgg cgatcctgcc cgtgctgttc atcgtgctcg gtctcgagga gaccgcgaag
     3481 atctcgctca tcgtcatcgg tatcgcgccg tgtatcgcgc gcgatctcgc gctgcgagtg
     3541 ggtgaactgc ccaaggagca gatcatcaag gcgcagacgc tgggtgcgtc gacgtggcag
     3601 ttgatccttc gcgtggtgct gccgcagctc tggccgcggt tgatagattc cttgcggctg
     3661 tcgatgggct cggcatggct gttcctcatc gccgccgaag ccatcgcgtc gaccgagggt
     3721 ctcgggtacc ggattttcct cgtgcggcgc tatctcgcca tggacgtgat cctgccgtac
     3781 gtcgtgtgga tcacgctgct ggccgtcgcc accgacttgg ggttacgtgt cctgcgcgcc
     3841 cgcgcgtttc gctgggccga accggaacgc tgatggcaac ggtgacggtt cgaaacgtgt
     3901 ggaaggaata cggcgcgcag gtcgtgctcg agaacgtgcg gctcgagatc gccgatcacg
     3961 agttcgtgac gattgtcggt gcctccggct gcggcaaaac gacgttcctc aagatgttgt
     4021 taggcatgga gcagccgacg cgcggccaga tcctcatcga cggcgagccg atccggcccg
     4081 agcccgaccc cgaccgtggc gtggtgttcc agcgttactc gtcgtttccg cacctgaccg
     4141 tggtcgagaa cgtgatgctc gggctcgagc tgcggtcctc gcggctcgcc ggcaagttgt
     4201 tcggcgcggc gcgacgcgaa gcgcgcgacg cggcgctggc gatgatcgag caggtgggcc
     4261 tcacccacgc gcgcgaccgc tacccggcgc aactctccgg cggcatgcaa cagcgcatgt
     4321 cgatcgcgca ggccgtgatc cgcaaaccca agatcctgct gctcgacgag cctttcggcg
     4381 cgctcgatcc cggcgtcacg gcggacatgc acgaactcat cctgcgattg tgggaagaga
     4441 accgcatgac catcttcatg gtgtcgcacg acatccagga aagcttccag ctcggcacgc
     4501 gcctgctcac gttcgacaag ctgcggcacg acccgcagtc gcccgaggcg tatggcgcga
     4561 gcgtgaccta cgatctgccg ctcacgcggg cgaaggccgc ttcgccgtaa atcgcgcaaa
     4621 tgtcgcgcgg cgcgaaacgc gcttcgccgc tggcgatgcc ccatctaccg gacggagcgg
     4681 cattcgagct cgctaactcc ccgcgcccat tggaaaaacg cggctgtccg cgcgtaacgc
     4741 ccgcatcctg gcacgcgcgc tgcatttctc ctgctcatgc aaacagcggc aggaagcccc
     4801 ttcagcacgg caacggagat ggaactcgca tcgcggttcc tcgaagcgac ggacgctccg
     4861 cgcctgcaac acgccatcag cgcactcatc aaacgcgccc gtgatgccag cggccgcaag
     4921 ctcgatgccg ccaccgctcg ccgcctccgg caacagcttc tcgacaccgc gacccgtctc
     4981 ggatttccgc agatcggccg cctgcgccac gccgcgatcc gcccaccgca ggtcgcccgg
     5041 ggcatgggcg tgctgtcgcg cgctgcgcgc tcgttaggcg tggagctcga gggcctgagc
     5101 cccgaggaca aggaattcga aatcgcgcgc cgcttcgtcc gcctcggcgg ccagacggcg
     5161 cgccgcctgc ggcgcgagcc cgcatcccct tcatgcgcct gccagggcag ctgcggttgc
     5221 agcagctgcg gcggcgcacc tgccgacatt cccgacactt caaacaggag tcacgccatg
     5281 cacgacatcg atcacaccaa atccgagtac gaccccgaac tggacgagct cgatgagctg
     5341 gagaccgacg agatggaatt ctcggacgag ttcgattccg gcgaactgga cgaaatggaa
     5401 accgacgccg gtgaatacga cagcggtgag ctcgacgaac tggagctcga cgagacgtcg
     5461 ggacccttca gcgaagtcga ggaagcggaa ctcgcgcagg agttgctggg cctctccagc
     5521 gaagccgagc tcgatcagtt cctcggcaag atcttcaaga aggcctggcg cggcgtacgc
     5581 ggtctcggca agacactggg ccgtgtcgcg cgtccgctcg gcggcatcct caagggcgtc
     5641 gccaagaaat tgctgcccgt ggccgcgggt gctgccggca cgttcttcgg cggtcccgcg
     5701 ggcgcggcca tcggctcgaa gatcggctcc gtcgtcggca acgcgctcga ggccgaattc
     5761 gaaggcagtc ccgaggatcg cgagttcgaa gcggccaaga ccttcgttcg cctggcgggc
     5821 aatgccgcgc agcaggcggc gaatacctcg ccgggcgcgc cgcccgtgtc gacggcaaaa
     5881 aatgcggtgg tcagcgccac acgaaacagc gtgcagcgac tgcgccgccg ccgctcgcgc
     5941 ggagcgtcca actaccacgg cggcagccgg cgcacgggcc gatgggtgcg ccgtggcaat
     6001 cgcatcatcc tgttcggagt ctgagccgcg atgacgcccg acgcccacag gttgctgacg
     6061 caggaagcac gggctttgct cacgcggctg gccctggtga agccgctggt gctgcaggag
     6121 acgctggtgc ccgcggccaa tgtctccgac acggcggcgg cgggcatcga acgcgcgctc
     6181 gccagcgggc ggcgcacggt gcgcacgctg gtgaacggct tcctcggctg gttgcgaggc
     6241 gagggcggcg cgcatgcaac ggatgcgcag gcgcagcggc ggttctcgct ggtgcgtctg
     6301 cagttcaaca ccatgctcgc gcagttcgac atcttcgcgg atgcctactc ccagcgcagc
     6361 gaacacgaca tcggcgtgtg gcttgccggg ctcgacgtgg cggcggcgga tgcgctcgcg
     6421 cttccgcggt attaccgctc gccggcgctg gtctgttatc tcgaccgcgg catcggcgcg
     6481 gcgattcgtc gcgcgcgc
//
LOCUS       DATDEU010000687         8389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_423428, whole genome shotgun sequence.
ACCESSION   DATDEU010000687 DATDEU010000000
VERSION     DATDEU010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423428"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1008)
                     /locus_tag="VIV63_16440"
     CDS             complement(115..1008)
                     /locus_tag="VIV63_16440"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY6126243.1"
                     /translation="MLSRRSFLVSAGAGMAAGSLASSIAFANHHIKIPLGIQLWTVKS
                     EAEKDLEGTLRKVYAAGFRDIEFAGYYGKSPADLAKLMKDIGFSLVSTHAGAGDIAKN
                     GDKIIADAKALGLKYVVCSSPGVSPEKEKLPWEERMKAVDLTDWKWNADLFNKFGKKV
                     SDAGMLFGYHNHSAEFKKYDGKTAFDHLYSMTDPKYVKIEMDIGWVVVAQQDPVALLN
                     QYKDRVIALHVKDVAKRGADGKDPSSVALGEGTIDWKKVLGTAHAHGVKHYFYEQEEP
                     FTRPILESVKISGDYLTKLAI"
     gene            1168..4806
                     /locus_tag="VIV63_16445"
     CDS             1168..4806
                     /locus_tag="VIV63_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HEY6126244.1"
                     /translation="MTNRLEGVPAEWADLVLQKLRTMEYSPTGGGPSDWPERILNFVL
                     TGRSGDVFDEIRDIRRKHALMLSRMHHAGDAEKDLYGNFGGLPADVCLRWSQAILAIH
                     ETGGFTHDLYFDLPKELAGLEAMFMHACGTQPSTWPMGREQPLTYRAIEKTTAAAGYP
                     ESAFMVSAFSTALGSSERLALITLMPDYADALDRHLESVRPHLRPTELNRRLHVLKLL
                     KSAEPRTLDRVAEELVVHAGATSKNARLAADELIARCGAPIVAPLRAMALEGNPEQRL
                     RALRLLRDHAKRHADAGLEEFARATARADSNASVQALEAEWQATAQDANEPPPLEYEI
                     PVIDWSLGYSEDIEALLQAFAKNYDAVAAENNRKRVATNARNEAEGVEWRQALLENFS
                     EENLASLRALLRDPAPAKRGVVYSNQKHGYEFADLQAGLGRAPGMTPEALFKILHFTG
                     SLVGGADYQLWYRAPAAFDAMHARTGRPTLLELAQMLNDSGFEGAAILNNYCSRFAGG
                     IARRWDGAAVWPYFAHYREQLIARLNQPDLRNYSFDRPSLFKAICTLPFPLPEMIDPL
                     FDIALGTTRNERELAQQALARYPRRRERIVSALRDGRAEVRTVAAQWLGRAGEAEGVA
                     PLAEAFAREKHDTTRGAMLDALEALGEPVEKYLDRDKLVAEARKSASKPLPAEIAWLP
                     LGTLPEIRWAASGEIVPPELPRWMLAQACRAKSPEPNAILRKYCALFDAGDRERFGQW
                     VLENWLHEDVVPISPEEAAKRAHTDAQQMHAAIAQWPQGYENSPMLGKTVVELTQMFL
                     PGLLRQPAGSAIGSKGILAVAAACAAQRAAAPVARYIKEWYGTRAAQGKALIAMLAWI
                     EHPTATQLMLAIGNRFRTRSFQEEASRQAAALAERRGWTLAELADRTMPGAGFDEHGV
                     LELPAGDRTFTAKLLPDFTVELFNPDGKKIASLPESAKDSRKLVSAAKKELKSIVELQ
                     TDRLYEALCTGRDWSFGDWRAYLLDHPVVRHLLQRLVWIGTPAGQGPQVFRPLDDGTL
                     TDCDDEAVTMPDDARVRLAHDSLLTPEQVGRWQQHLIDYEIRPPFQQLGKGTWSLPEA
                     LATNSAIEEFKGHMLEAFALRGRAQKLGYVRGTAEDGGWFYDYRKRFPTLGLEAQIRF
                     SGNGLPEENRKVALIELSFSATGEASSDPVTLGSIPGVLLSECYNDLRLIAAEGTGFD
                     PEWESKTG"
     gene            4821..>8389
                     /locus_tag="VIV63_16450"
     CDS             4821..>8389
                     /locus_tag="VIV63_16450"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HEY6126245.1"
                     /translation="MRDWLKGMFGGSAPAPDGIPENWVGELKTMLAEAFVVGGTNLSG
                     KNLDDAVEFVLRGTAGNWTHPVPPRNPQGYISYNNAERPEQPAFYAALGDLPGDLLLR
                     WAQVLESMAFIRQYSPFQMIFPGAVHWPEIVLMYGSGSNMNVYPPKVANQRIAYSTLE
                     KMLVAAGMDPQELLVRSFATPVDVGYNAGALLLAVSHCPDYADALDRHIESLRPHLLA
                     GSAAQRVHVLGMMARAAPRTVDKLAHELAELSVASSKQVRMAAESFVQSAGGPMIATL
                     RQLAVEGKPEQRQFALRHLFDRGRKGNDPALCDFARTTAAADKAASVQALPAEWDTAK
                     NADEAPEAKYDYQMPVIDWSGTLDAALAARLDKLWEDINALILADNQRSREYYERHSQ
                     QHGKPQWKLHQNKEISSSWLKELKSELDSGKPPGKESKRSMQRVWVALPAVHNFATDA
                     SLEPTQLWKVLHFLSMLFDHRSSLNNIAVESFNRMYAANSRPTLIEIGQMAEDTGFTA
                     TGIFASYCSSHGALARTWKAEDVWPFVAHHVPTIERYLLGLEQTDYWFSREMLYHAIS
                     VLPTPPPRIVNALFTVALGQGKSERLNAQEALANLPNKEARIIAALSDGKSEIRMVAA
                     QWLGRLKHEPAIPALSKAVATEKHDTAKGAMLDALQILGEPVDKYLKRDALLPEAKKA
                     LAKPIPKDLDWVPWDTLPAVRWADNGQAVAPDVLKWMVVQACRAKSAEPNAVLRKYCA
                     MFEPRDREAFGQWVLDNWLREDIAPIPAEQAHSSARASAHATHGSMKSHPQYYQNHPM
                     LGKSEDEIFASMLPSFLRQPKGSAIGSKGILAVAASCAAERAAAPVARYIKEYYGTRA
                     AQGKALITMLAWIEHPSATQVMLAIGNRFRTKSIQEEATRQAEALADRKGWTLAELAD
                     RTIPSAGFDENGELELSYGNRAFVAKLLPDFKIELFNPEGKKVASLSEPRQDDDADRA
                     QESKKALSAAKKEIKNIVALQTDRLYEALCTGREWPYADWHDYLNLHPVVRHLAQRLI
                     WLQYADSGVVNVFRPLDDGTLTNADDEAVTLPPDARVRLAHDSLLPREAVERWQQHLV
                     DYEIKPLFQQLGKGTYELPPDRQNAKELSDFQGHLLEAYALRGRAGKLGYQRGAAEDG
                     GWFHVYEKRFPTLGLTAVVQFTGSPLPEENRTVALINLSFSSS"
BASE COUNT         1581 a         2774 c         2675 g         1359 t
ORIGIN      
        1 ttggcccact tcgccatgtc attgagattc tgcagatgcg ggccaccgcc cgcgaactgc
       61 gccaggaata ccgcaggtcc cttgatcgtc ttcacttccg cgccccagag atagttagat
      121 ggcgagcttg gtcaggtagt cgccgctgat cttcaccgac tcgaggatgg ggcgggtgaa
      181 cggctcttcc tgctcgtaga aatagtgctt cacgccatgg gcgtgcgcgg tgccgagcac
      241 cttcttccag tcgatggtgc cttcgcccag cgccaccgag ctcgggtcct tgccgtcggc
      301 gccgcgcttg gccacgtcct tgacgtgcag cgcgatcacg cgatccttgt actggttgag
      361 cagcgcgacg gggtcctgct gcgcgaccac cacccagccg atgtccatct cgatcttcac
      421 gtacttcgga tcggtcatgg aatacagatg gtcgaaggcg gtcttgccgt cgtacttttt
      481 gaactcggcg ctgtggttgt gataaccgaa cagcatgccg gcatccgaca ctttcttgcc
      541 gaacttgttg aagaggtcgg cgttccactt ccagtcggtg agatccaccg ccttcatgcg
      601 ctcttcccag ggcagctttt ccttctcggg cgagacgccc ggcgaggagc agacgacgta
      661 cttgaggccg agcgccttgg cgtccgcgat gatcttgtcg ccgttcttgg cgatgtcacc
      721 cgcgccggcg tgcgtgctga ccagcgagaa gccgatgtcc ttcatgagct ttgcgagatc
      781 ggccggagac ttgccgtagt agcccgcgaa ttcgatgtcg cggaagcccg cggcatagac
      841 cttgcgcagc gtgccttcga ggtctttttc ggcttcggac ttcaccgtcc acagctggat
      901 gcccagcggg atcttgatgt ggtgattcgc gaaagcgatg gaggaggcta atgatccggc
      961 cgccataccg gctcccgcgg acacgagaaa agaacgtctg ctcagcatga acgacccctc
     1021 ttgaatgtgt ggcccgggcc cgtcctgggc tccgatccgg acgcgggagt acacggcatc
     1081 gggacgccag ccgtcaagaa tgcaaaacct tggccaaggc gccgggcgtt gagacatgat
     1141 ccagcgacaa aaataagggc acgtatcgtg acgaaccggc tcgaaggagt gcccgcggaa
     1201 tgggccgatc tggtgctcca gaagctccgg accatggaat acagcccaac cggtggcggc
     1261 ccctcggact ggccggagcg aatcctgaat ttcgtgctga ccggccggtc cggcgatgtg
     1321 ttcgatgaaa tccgcgacat ccggcgcaaa cacgcgctga tgctgtcccg catgcaccat
     1381 gcgggcgatg ccgaaaagga tctgtacgga aatttcggcg gcttgccggc cgacgtctgc
     1441 ctgcgctgga gccaggcgat tctggccatc cacgagaccg gtggcttcac gcacgacctc
     1501 tacttcgatt tgccgaaaga gctcgcgggg ctcgaggcga tgttcatgca tgcgtgcgga
     1561 acacagccca gcacctggcc catgggccgc gaacagccac tgacttatcg cgccatcgag
     1621 aaaaccacgg ccgccgccgg ataccccgaa agcgcgttca tggtttccgc gttctcgacc
     1681 gcactgggtt cctcggaacg cctcgcactg atcacgctca tgcctgacta cgccgatgcg
     1741 ctcgatcgcc atctggaatc ggtgcggcca catctgcggc cgacggaact caaccggcgt
     1801 ctgcacgtgt tgaagctgct caagtccgcg gaaccgcgaa cgctggatcg ggtggcggaa
     1861 gagctcgtgg tacacgcggg cgcaacctcc aagaatgcgc gcctcgcggc cgacgaactc
     1921 atcgcgcgct gcggcgcgcc catcgtcgca ccgctgcgcg cgatggcgct cgagggcaat
     1981 cccgagcagc gcctgcgggc gttgcgactg ctgcgcgatc atgcgaaacg ccacgcggat
     2041 gcgggcctcg aagagttcgc gcgcgccacc gcccgtgcgg actcgaatgc gagcgtgcag
     2101 gcgctcgaag ccgagtggca agccacggcg caagatgcga acgagccgcc accgctcgaa
     2161 tacgaaattc cggtcatcga ctggtcactt ggctattccg aagacatcga agcgttgctg
     2221 caggcgttcg cgaagaacta cgacgccgtg gcggcggaga acaatcgcaa gcgcgtggcg
     2281 accaatgcgc gcaacgaggc cgagggcgtt gagtggcggc aggcgctgct cgagaacttc
     2341 tccgaggaga accttgccag cctgcgggca ctgttacgcg acccggcgcc cgcgaaacgc
     2401 ggggtggtct acagcaacca gaaacatgga tacgaattcg ccgatctgca ggccggactc
     2461 gggcgggcgc ccggcatgac tcccgaagcg ctcttcaaga tcctgcactt cacgggctcc
     2521 ctcgtcggcg gcgcggacta ccagctctgg taccgcgcgc cggcggcttt cgatgcgatg
     2581 cacgcgcgca ccggtcgccc cacgctgctc gagcttgcgc agatgctgaa cgacagtggt
     2641 ttcgagggcg ccgcgatcct gaacaattac tgctcgcgtt tcgcaggggg aattgcccgc
     2701 cgctgggatg gcgcagccgt ttggccgtac ttcgcgcact atcgcgagca gctcatcgcc
     2761 aggctcaacc aacccgacct gcgaaactat tctttcgacc ggcccagtct cttcaaggcg
     2821 atctgcacac tgccttttcc gctgccggag atgatcgacc cgctgttcga catcgccctc
     2881 ggcaccacgc gcaacgaacg cgaactcgcg cagcaggcac tcgcgcgata tccccgcagg
     2941 cgcgagcgca tcgtcagcgc gttgcgcgac gggcgcgccg aagtacgcac cgtggcggcg
     3001 cagtggttag gccgtgccgg cgaggcggaa ggcgtggcgc cgctggccga agcgttcgcc
     3061 cgcgagaaac acgacacgac gcgcggcgcc atgcttgatg ctctcgaagc gttgggcgag
     3121 cccgtcgaga aatacctgga ccgcgacaaa ctcgtggccg aagcgcgcaa gtcggcatca
     3181 aagccgctgc ccgcggagat cgcatggctg ccgctcggca cgctgccgga aattcgctgg
     3241 gccgcgagcg gcgagatcgt cccgcccgag ttgccgcgtt ggatgctggc gcaggcgtgc
     3301 cgcgcgaaat cgccggagcc gaatgccatc ctgcgaaagt actgcgcgct gttcgacgcg
     3361 ggcgatcgcg aacgcttcgg ccagtgggta ctggagaact ggctgcatga ggacgttgtt
     3421 cccatctcgc ccgaagaagc cgcgaaacgc gcgcacaccg acgcgcagca gatgcatgcc
     3481 gcgatcgcgc agtggcccca gggctacgaa aacagtccga tgctcggcaa aaccgtggtc
     3541 gaactcacgc agatgtttct gccgggcctg ctgcggcagc ccgccggctc ggccatcggc
     3601 agcaagggca ttctcgcggt ggcagcggct tgcgccgcgc agcgtgccgc cgcgccggtc
     3661 gcgcgctata tcaaggagtg gtacggcaca cgcgctgcgc agggaaaggc gttgatcgcg
     3721 atgctggcat ggatcgaaca tcccaccgcc acgcagctga tgctcgccat cggcaaccgg
     3781 ttccgcacgc gaagtttcca ggaagaagcg tcgcgccagg ccgccgcgct cgccgaacgc
     3841 cgtggctgga cgctggcgga actggccgac cgcaccatgc cgggcgccgg cttcgacgaa
     3901 cacggcgtac tcgaactgcc ggcgggcgat cgcacgttca ccgccaaact actgccggac
     3961 ttcaccgtcg agctgttcaa cccggacggc aagaagatcg cttcgctgcc cgagtccgcc
     4021 aaggattcgc gcaagctcgt cagcgccgcg aagaaggagc tcaagagcat cgtcgagctt
     4081 cagaccgacc ggctctacga agcgttgtgc acggggcgtg actggtcttt cggcgattgg
     4141 cgcgcgtatc tgctcgatca cccggtggtg cgccatctcc tgcagcgcct ggtctggatc
     4201 ggcacgccgg ccgggcaagg cccgcaggtg ttccgcccgc tcgatgacgg cacgctgacc
     4261 gactgtgatg acgaagcggt gacgatgccc gacgacgccc gcgtgcgcct ggcgcacgac
     4321 tcgctgctga cgcccgaaca ggtcggccgc tggcagcagc atctcatcga ctacgaaatc
     4381 cgcccgccgt tccagcaact gggcaagggc acctggtcgc tgccggaagc actcgcgacg
     4441 aattccgcca tcgaagagtt caagggccac atgctcgagg cattcgcgct gcgcggccgc
     4501 gcgcagaagc tgggttatgt ccgtggcacc gctgaagacg gcggctggtt ttatgactac
     4561 cgcaaacgtt tcccgacgct cgggctcgaa gcccagattc gtttctcggg caacggcctg
     4621 cccgaagaaa accgcaaggt ggcgttgatc gagctgtcgt tctccgccac gggcgaagcg
     4681 tccagcgatc ccgtcacgct cggcagcatt cccggagtgt tgctctccga gtgttacaac
     4741 gacctgcgcc tgatcgccgc cgaaggcacg ggcttcgatc cggagtggga aagcaaaact
     4801 ggttaggcga tgatccgggc atgcgcgatt ggctgaaggg aatgttcggc ggctcggccc
     4861 ccgccccgga cggcatcccc gagaattggg tgggcgaact caagaccatg ctcgccgaag
     4921 cattcgtcgt cgggggcacc aacctttccg gcaagaatct cgacgacgcc gtcgaattcg
     4981 tcttgcgggg caccgcgggc aactggacac atccggtgcc gccgcgcaat ccgcagggct
     5041 acatcagcta caacaacgcc gagcggcccg agcagcccgc gttctacgcc gcgctcggcg
     5101 atctgccggg cgacctcctg ctgcgctggg cgcaagtgct cgaatccatg gcgttcatcc
     5161 gccagtactc gccgttccag atgatctttc ccggcgccgt gcactggccg gagatcgtgc
     5221 tgatgtacgg ctcgggctcg aacatgaatg tgtacccgcc caaggtcgcg aaccagcgca
     5281 ttgcatacag cacgctcgag aagatgctcg tcgccgccgg catggatcca caggaattac
     5341 tcgttcgctc gttcgcgacg ccggtggatg tgggctacaa cgcgggcgcc ctgctgctgg
     5401 cagtttcgca ttgcccggac tacgccgacg cgctggatcg ccatatcgag tccctgcggc
     5461 cgcacttgct ggccggcagc gcggcccagc gcgtgcacgt gctcggcatg atggcgagag
     5521 cggcgcctcg cacggtggac aaactggcgc acgaactcgc cgaactatcg gtggcgtcga
     5581 gcaagcaggt acgcatggcg gcggaaagct tcgtgcagtc ggccggcgga cccatgatcg
     5641 ccacgctgcg ccaactcgcc gtcgaaggta aacccgagca gcgtcagttc gcgctgcgcc
     5701 atcttttcga tcgcggccga aaaggcaacg acccggcgct ttgcgacttc gcgcgcacca
     5761 cggccgcggc cgacaaggcg gcttcggtgc aggcgctgcc ggccgaatgg gacacggcga
     5821 agaacgccga cgaagcgcct gaagcgaagt acgactacca gatgccggtc atcgattgga
     5881 gcggcacgct ggatgccgcc ctggccgcgc ggctcgacaa gctgtgggaa gacatcaatg
     5941 ccctgatcct cgccgacaac cagcgctcgc gcgagtacta cgagcggcac tcgcagcaac
     6001 acggcaagcc gcagtggaag ttgcaccaga acaaggaaat ctcctcatcc tggctcaagg
     6061 aactcaagag cgagctcgac tcgggcaaac cgccgggcaa ggaatcgaag cgctccatgc
     6121 agcgcgtctg ggttgcactt cccgccgtgc acaacttcgc caccgacgca tcgctcgagc
     6181 ccacgcagct ctggaaggtt ctgcatttcc tctcgatgct tttcgatcat cggtcgagtc
     6241 tcaacaacat cgcggtggag agcttcaacc gcatgtatgc cgccaacagc cggcccacgc
     6301 tcatcgaaat cggccagatg gccgaagaca ccggctttac ggccacgggc atcttcgcca
     6361 gctactgctc gagccacggc gcgctcgcgc gcacgtggaa ggccgaagac gtgtggccgt
     6421 tcgtcgctca tcacgtgccc accatcgagc ggtacctgct cggcctcgaa cagacggact
     6481 actggttcag ccgcgagatg ctctaccacg ccatctccgt gctgcccaca ccaccaccgc
     6541 gcatcgtcaa cgcgttgttc acggtggcgc tcggccaggg caagagcgag cggctgaacg
     6601 cgcaggaagc actggcgaac ctccctaaca aggaggcgcg catcatcgcc gcgctgtccg
     6661 atggcaagtc cgagatccgc atggtggcgg cgcaatggct gggacggctc aaacacgaac
     6721 cggcgatccc cgcgctgtcg aaggcggtgg ccaccgagaa acacgacacc gccaagggcg
     6781 cgatgctcga tgcgctgcag atcctcggcg aacccgtgga caaatacctg aagcgcgatg
     6841 cgctgcttcc ggaagcgaag aaggcgctgg cgaagccgat cccgaaggat ctcgactggg
     6901 ttccctggga cacgctgccc gcagtgcgct gggccgacaa cggccaggcg gtcgcgccgg
     6961 acgtgctcaa gtggatggtg gtccaggcct gccgcgcgaa gtccgcggag ccgaacgcgg
     7021 tgctgcgcaa gtactgcgcg atgttcgagc cgcgcgatcg cgaagcgttc gggcagtggg
     7081 tgctcgacaa ctggctgcgc gaggacatcg cgccgatacc ggccgaacag gcgcacagct
     7141 cggcgagggc cagtgcccat gcgactcacg gctcgatgaa gagtcacccg cagtactacc
     7201 agaaccatcc gatgctcgga aaaagcgagg acgagatctt cgcgtccatg ctgccgtcgt
     7261 tccttcgtca gccgaaaggc tcggcgatcg gcagcaaggg cattctcgcg gtggcggcgt
     7321 cttgcgcggc cgaacgcgcc gcggcgccgg tggctcgcta catcaaggag tactacggaa
     7381 cgcgcgccgc gcagggcaag gcgttgatca ccatgctggc gtggatcgag catccgagcg
     7441 ccacgcaggt catgctggcc atcggtaatc gcttccgcac caagagcatc caggaagaag
     7501 cgacacggca ggccgaagcg ctggccgacc gcaaaggctg gaccctggcc gagcttgcgg
     7561 accgcacgat tccgagcgcg ggcttcgacg aaaacggcga gctcgaactc tcgtatggca
     7621 atcgcgcctt cgtggccaaa ctactgcccg acttcaagat cgagctgttc aaccccgaag
     7681 gcaagaaggt cgcgtcgctc tccgagccgc gccaggacga tgacgcggat cgcgcgcagg
     7741 aatccaagaa ggcgctgagc gcggcgaaga aagagatcaa gaacatcgtc gcgctgcaga
     7801 ccgaccggct ttacgaagcc ttgtgcaccg ggcgagagtg gccctacgcg gactggcacg
     7861 actacctcaa tctccatccc gtggttcggc acctcgcgca acgcctgatc tggctgcaat
     7921 acgccgacag cggcgtcgtg aacgtattcc gccccctcga cgacggcacg ctcaccaacg
     7981 ccgatgacga ggccgtgacg ctgccgcccg acgcacgcgt gcggctcgcg catgattccc
     8041 tgctgccgcg cgaggcggtc gagcgctggc agcagcacct cgtcgactac gagatcaagc
     8101 cgctgttcca gcagctcggc aaaggcacct acgaactgcc gcccgacagg cagaacgcca
     8161 aggaactcag cgatttccag ggccacctgc tcgaggcgta cgcgttgcgc ggccgcgccg
     8221 gcaaactggg ctaccagcgt ggtgcggccg aagacggcgg ctggttccat gtgtacgaga
     8281 aacgcttccc cacgctcggc ctcactgccg tggtgcagtt cacgggcagt ccgttgccgg
     8341 aagaaaaccg cacggtggcg ctgatcaatc tctcgttcag ttcgagcga
//
LOCUS       DATDEU010000688         5914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_133349, whole genome shotgun sequence.
ACCESSION   DATDEU010000688 DATDEU010000000
VERSION     DATDEU010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133349"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VIV63_16455"
     CDS             complement(<1..342)
                     /locus_tag="VIV63_16455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6126246.1"
                     /translation="MNRSNNKGFTLIELMISMVLGLVVVGGVISVVLANRRTYNTNEG
                     LSQVQESARTAFELLARDIRQAAGNGCDNTTGVSNVLNTSTAWWRDWYGMEGFESSET
                     DSAVAQGPGVEL"
     gene            complement(339..791)
                     /gene="pilV"
                     /locus_tag="VIV63_16460"
     CDS             complement(339..791)
                     /gene="pilV"
                     /locus_tag="VIV63_16460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEY6126247.1"
                     /translation="MRMHIKEQGVGLIEVLVAVLILSIGMLGLAGLQMRTLRNSQSSL
                     ERGVAVVETHFIADAMRADLLSVEDGDYNIGLTDDPPTTPATFAEKAIASWRSNIEAN
                     MGDGATGSIDCNGRDCNIIIQWNDSRGSGNAADIAEREEFQIATQVRL"
     gene            complement(793..1320)
                     /locus_tag="VIV63_16465"
     CDS             complement(793..1320)
                     /locus_tag="VIV63_16465"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY6126248.1"
                     /translation="MRPLRSSRAGFTIVELMVTISLAAILLAVAIPSFRQMIASNRLT
                     TQINDFVSAVNFARSEAITRNLNVSFCRVDDPEDDECSTDDGVWRNWIVLGSNGTVMR
                     RGEVTDASGISVSSTLTSDQIVFGANGLSTTGGLLVSDSQITVCSDMATGTNIRQITL
                     GAGSRITTEKLSGAC"
     gene            complement(1597..1818)
                     /locus_tag="VIV63_16470"
     CDS             complement(1597..1818)
                     /locus_tag="VIV63_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126249.1"
                     /translation="MSSTKIIAIVLIVAGVLGLAYGKFSYTKETHDAKVGPIELSVKD
                     KETVNVPVWAGVGAIVIGAGLLLAGGKRA"
     gene            complement(1945..3291)
                     /locus_tag="VIV63_16475"
     CDS             complement(1945..3291)
                     /locus_tag="VIV63_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126250.1"
                     /translation="MTTTVRILGSRAREQVPGTGITLLAPGFRGALTQLAAGAATHTT
                     HDLPFAEAAAGAEAMLAAQLTLSIEARPADRAAGLRSRSAVASHPRVIVPRRGGLAYA
                     LLQTDETGTSSFVLPASGDVTEAVFPLTIAAHGSTRRMLRVLMWPAQLLSGGGVLAAA
                     ERWENLRRPNQIAQCGPGGDWLAPDWPALRQGPVLLLLHDTFSTPQSTFADWVGDDSF
                     TPVFRAFGGRCLAFAHPTLSCGLEENVAWLVSQVASLPGPFDIVALGRGGLLARAIAS
                     DGRLPLRRVCQVGTPNLGTPLAHEANLLRFLDGHVAMLACIPNRVARSTLEGVLCMAR
                     IAALGLHSPLPGVTIQATGNPALDALRGASPGPQWFTVSAQYSAVGGHGMRPLAAEDF
                     AATPNDLVVPSEGCHEPGAPVTDRLRLGGAQVHHHNYFANTHVRTHLAEWLRTEEM"
     gene            3552..5594
                     /locus_tag="VIV63_16480"
     CDS             3552..5594
                     /locus_tag="VIV63_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEY6126251.1"
                     /translation="MSNVTRALRRASILCTFLLLPVVSFAGFSVSNGQLVDNNGTPFV
                     MRGINYPYTWYQSRPTQQDFAAMAATGANTVRLVLSTGGQWPRVTGTQLSQLIQWSRD
                     NRMIAVLEVHDSTGWTESTAAVPISNATAYWTSADIRAAINGQENFVIINIANEPFGN
                     TTTANYTPDTIAAIQALRSAGLTHTLMVDAANWGQDWSNTMRTNAMQIWNSDTRRNLV
                     FSVHMYEVYAQASAVTSYMQAFDDMALPLVVGEFGADHQGQAVDEAAIMAEAQARGNG
                     YIGWSWSGNGSCCTSLDIVSNFSSTFTSWGNILVNGTNGIRATSVLATVFGTPTNNLT
                     VAPSTLSFASGAASSPVTVTANVSWTVTDNQTWITASPTSGTNNGSFTVSATANTGTA
                     SRSGTVTVTGGGLTRTIAVTQAAATANNLTLSQTSLSFASTAASSAVNVTANVSWTIT
                     DDQTWLSASPTSGSNNGSFTVSATANTGTASRTGAVTVTGGGLTRTVSVTQAGQSTTN
                     TLTVSTTSLALASAISSGTFSITSNVSWAVTDDQSWITVTPASGSNNATVTVNATSAN
                     TATTSRTGTVTVTGGGLTRTVSVTQAGSGGTGGTCPNPVTFNGNTGNFNTTGAACYRT
                     NANISGWGCYNFDGRTLTVGGVARTCGQMPLTRSADGYYYFSATAGQFPWAGLYTW"
     gene            complement(5636..>5914)
                     /locus_tag="VIV63_16485"
     CDS             complement(5636..>5914)
                     /locus_tag="VIV63_16485"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY6126252.1"
                     /translation="VFESTLAFQAQMICNLLLAAGVPARVDGSYLQSIGGEIPLGNAV
                     RVRVEADRAAEARAIIAEWERAPIPSDEEAAAEANAAGLGAGPQPPTG"
BASE COUNT         1064 a         1930 c         1923 g          997 t
ORIGIN      
        1 aagctcgacg cccggaccct gggccaccgc ggaatccgtt tccgagcttt cgaaaccttc
       61 catgccgtac cagtcgcgcc accaggccgt gctggtgttc agcacgttcg acacgccggt
      121 ggtgttgtcg caaccgttgc cggccgcctg gcggatgtcg cgcgccagga gttcgaacgc
      181 ggtgcgcgcg ctctcctgca cctgcgacag gccttcgttg gtgttgtagg tgcggcggtt
      241 cgccagcacg acgctgatga cgccgccgac gacgaccagg ccgaggacca tcgagatcat
      301 caactcgatg agcgtaaagc ccttgttgtt tgagcggttc acagtcgcac ctgcgtggca
      361 atctggaatt cctcgcgctc tgcaatgtcc gcggcgttgc ccgacccgcg cgaatcgttc
      421 cattggatga tgatgttgca gtcgcggccg ttgcagtcga tcgagccggt ggctccgtcg
      481 cccatgttcg cttcgatgtt cgagcgccag gaggcaatcg ccttctcggc gaatgtcgcg
      541 ggggtcgtgg gcggatcgtc ggtcagcccg atgttgtagt cgccatcttc gacgctgagc
      601 agatccgcgc gcatcgcatc ggcgatgaag tgagtctcga ccacggcgac gccgcgctcg
      661 agcgaactct gactgttgcg cagcgtgcgc atctgcagcc cggcgaggcc gagcatgccg
      721 atggaaagaa tcagcacggc gaccagcact tcgatgaggc cgacaccctg ctccttgatg
      781 tgcatccgca tgtcagcaag ctcccgaaag cttctcagtg gtgatacggc tgccggcgcc
      841 gagcgtgatc tgccggatgt tggtgcccgt ggccatgtcc gagcacaccg tgatctggct
      901 gtcggagacc agcaggccgc cggtggtcga caggccgttg gcgccgaaga cgatctgatc
      961 gctggtgagc gtggacgaga cgctgatgcc gctcgcgtcg gtgacttcgc cgcgacgcat
     1021 gacggtcccg ttggagccga ggactatcca gttccgccag acgccatcgt cggtggagca
     1081 ctcgtcgtct tccgggtcgt cgacgcggca gaaggagacg ttgaggttgc gcgtgatggc
     1141 ttcggagcgc gcgaagttga cggcggagac gaagtcgttg atctgcgtgg tcaggcgatt
     1201 cgaggcgatc atctgccgga acgacggaat tgcaaccgcg agcaggattg cggccagcga
     1261 gatcgtcacc atcagttcaa cgatggtgaa gccggcgcgc gatgaacgta ggggtcgcat
     1321 cagatatcca tcctcggttc ggagacttca gggcaatgcc tgaccgtgac tctccgcgag
     1381 gcggggacga ggcgcagctc cgttccgacc aacggccggg aagcagcgac tagcggcgcg
     1441 aaaaccgcga tttcgatgga ttaagtcggg tgggaaaggc cgttggggcc ggtacgaaat
     1501 gtgacgcagt tccggttcga cgcccggata aattgagagc ggccccgcac cacaaggcgg
     1561 gaccgctctg ctgacttact tctggcgggc tgaccgtcag gcgcgtttgc cgcctgcgag
     1621 cagtaatcct gcgccaatca cgatcgcgcc gacgccggcc caaacgggga cgttgacggt
     1681 ttccttgtcc ttcactgaca actcgatggg acccaccttc gcgtcgtgcg tttccttggt
     1741 gtagctgaac ttgccgtacg ccaggcccag tacgcccgcg acgatcagga cgatggcgat
     1801 gatcttggtg gaactcattt tgtatttgcc tgttgtgact tcggggtcga tgccgctgta
     1861 ggccggcacc gactgggtag gagattgctc tccgcgcccc ctcacaacca tcgccatgcg
     1921 tcgcatgtgg cgctcagcgg aatcctacat ttcttccgtc ctcaaccact cggcgagatg
     1981 cgtgcgcaca tgcgtgttgg cgaagtagtt gtggtgatgc acttgcgcgc caccgagtct
     2041 cagccggtcg gtcacgggcg cgcccggctc gtgacaccct tcgctcggca cgacgagatc
     2101 gttcggagtg gcggcgaaat cttccgcggc gagtggccgc atgccgtgtc cacccactgc
     2161 cgagtattgc gcgctcaccg tgaaccactg cggcccgggc gatgcgccgc gcagcgcatc
     2221 gagcgcggga tttccggtgg cctggatcgt cacgcccggc agtggcgagt gcagacccag
     2281 cgccgcgatc cgcgccatgc acaacacacc ttcgagcgtg gatcgcgcca ctcgattggg
     2341 aatgcaggcg agcatggcga cgtgcccgtc gagaaaacgc aggagattgg cttcatgcgc
     2401 gagtggcgtg ccgaggttgg gtgtgccgac ctggcagacg cgccgcaggg gcagccggcc
     2461 atcggaggcg atcgcgcgcg ccagcagtcc gccgcgcccg agggccacga tgtcgaatgg
     2521 tcccggcaga ctcgccacct gcgataccag ccacgcgacg ttttcttcga gcccgcagga
     2581 aagtgtggga tgggcgaacg ccaggcaccg tccaccgaaa gcgcgaaata ccggtgtaaa
     2641 cgagtcgtca cccacccagt cggcgaaggt cgattggggc gtggagaagg tgtcgtgcaa
     2701 cagcagcagc accggaccct gacggagcgc cggccagtcg ggcgcgagcc agtcgccgcc
     2761 cggaccgcat tgtgcaatct ggttgggcct ccgcaggttc tcccaccgct ccgccgcggc
     2821 cagcacaccg ccaccggaca gcagctgggc gggccacatc agcacgcgca acatgcggcg
     2881 ggtcgagcca tgtgcggcga tagttagggg gaagaccgct tcggtcacgt cacccgaggc
     2941 gggcagcacg aaactcgaag tgccggtctc gtcggtctgc agcaacgcat acgcaagccc
     3001 gccgcgacgc gggacgatca cgcgcggatg cgatgccacc gcgctgcgcg atctcaaccc
     3061 cgcggcccga tcggccgggc gcgcctcgat gctcagcgtg agttgcgccg ccagcatggc
     3121 ctcggcgccc gcggccgctt cggcgaacgg caggtcatgc gtggtgtgcg tggcagcgcc
     3181 ggcggcaagc tgcgtgagtg cgccgcgaaa tccgggcgcg agcagggtga tgccggtgcc
     3241 gggcacttgt tcgcgggcgc gtgaccccag gattcgcacc gtggtcgtca ttgcgatcga
     3301 ggctagtgca acctcgcgcg agtagatagt tgcggaactc cgcaatcgcg gatgcccatg
     3361 cgggcacggc ctaggaaact cccccgactg tgttagcgca accagcgcgg acaacgagcc
     3421 agacatcggg acgaatcgcg ggacaataac tatttttgct tgcgttaagc cgggcggagg
     3481 cgcatatcat cgcgccgcgg ctcagtacgt atggatgcgg ccgtatagac acggaacttc
     3541 agggggaagt catgagcaac gtcactcgcg cgcttcggcg cgcttcaatc ctttgcactt
     3601 ttcttttgct gccagtcgtc agtttcgccg ggttttccgt ctcgaacggc cagctcgtcg
     3661 acaacaacgg cacaccgttc gtgatgcgcg gcatcaacta cccgtacacg tggtaccaat
     3721 cgcgccccac gcaacaggat ttcgcggcaa tggccgccac cggcgcgaac accgtgcgac
     3781 tcgtactgtc gacgggcggc cagtggcccc gcgtcaccgg aactcagctc agccagctca
     3841 tccagtggtc cagggacaac cgcatgatcg cggtgctcga ggtgcatgac tcgaccggat
     3901 ggaccgagtc gacggcagcc gtgccgatct ccaacgccac ggcctattgg accagcgcgg
     3961 atatccgcgc cgccatcaac gggcaggaaa acttcgtcat catcaatatc gcgaacgagc
     4021 ctttcggcaa taccacgacg gctaactaca cgccggatac catcgccgca atccaggccc
     4081 ttcgcagcgc cggtctcacg cacaccctca tggtggacgc ggccaactgg gggcaggact
     4141 ggtcgaacac gatgcgcacg aacgccatgc agatctggaa ctcggacact cgccgcaacc
     4201 tggtcttcag cgtgcacatg tacgaggtct acgcgcaggc gagcgccgtc acttcctaca
     4261 tgcaggcctt cgacgacatg gcgctgcccc tcgtcgtcgg ggaattcggc gctgatcacc
     4321 agggacaggc cgtcgacgaa gcggccatca tggccgaggc gcaggcgcgc ggtaacggct
     4381 acatcggctg gtcgtggtcg gggaacggca gctgctgcac ctcactcgac atcgtgagca
     4441 atttcagttc gacgttcacg agctggggca acatcctggt gaacggcacg aacggcattc
     4501 gcgcgacctc ggtgctggcc accgtgttcg gcacaccgac caacaatctc accgtggcgc
     4561 cgtcgacgct gtcgtttgcc tcgggagccg cgtcgtcacc cgtcactgtg acggcgaacg
     4621 tgagctggac ggtcacggac aaccagacct ggatcacggc ctcaccgacg agcggcacca
     4681 acaatggcag cttcacggtg agcgccacgg ccaatacggg cacggcgtcg cgctccggca
     4741 cggtgaccgt gaccggcggt ggactcacgc gcacgatcgc cgtgacgcag gcggcggcga
     4801 cggctaacaa cctcacgctg tcgcagacct cgttgtcgtt cgcctcgacc gcggcctcgt
     4861 cggccgtgaa tgtcacggcg aacgtgagct ggacgatcac cgacgaccag acctggctca
     4921 gcgcctcgcc cacgagcggc agcaacaacg gaagcttcac ggtgagcgcc acggcgaata
     4981 ccggaaccgc gagccgtacg ggcgcggtga cggtgaccgg cggcggactc acgcgcacgg
     5041 tatccgtgac gcaggccggc cagtcgacta ccaacacgct caccgtctcg acgacgtcgc
     5101 tcgcgctggc atcggccatc tcgtccggca cgttctccat cacgtcgaac gtgtcctggg
     5161 ccgtgaccga tgatcaaagc tggatcacgg tgactccggc gtcgggcagc aacaacgcca
     5221 cggtcacggt gaacgcgacg tcggcgaaca cggcgaccac gagccgcacg ggcaccgtga
     5281 cagtgaccgg cggtggtctg actcgcacgg tgtccgtgac gcaggcgggt tccggcggta
     5341 cgggcggcac gtgccccaac ccggtcacgt tcaacggcaa caccggcaac ttcaacacca
     5401 cgggcgcggc gtgttatcgc accaacgcca acatcagtgg ttggggctgt tacaacttcg
     5461 acggcagaac gctcaccgtc ggcggcgttg cgcgcacctg cggacagatg ccgctcacac
     5521 gttcggcgga cgggtactac tacttctcgg cgaccgccgg acagtttcca tgggcgggcc
     5581 tgtatacctg gtaagcggta aggggccact gtgcggagtg gccccttttt tcaaactatc
     5641 cagtcggcgg ctgcggcccg gcgcccaggc cggctgcgtt cgcttcggcc gctgcttctt
     5701 cgtcgctcgg aatgggcgcc ctctcccact cggcgatgat ggcccgcgcc tccgcggcgc
     5761 gatcagcctc gacgcgaaca cgcacggcat tgccgagcgg aatctcaccg ccaatgcttt
     5821 gtagatagga gccgtcgacg cgcgccggca cgcctgcggc cagcagcagg ttgcagatca
     5881 tctgcgcctg aaacgcgagc gtcgattcga aaac
//
LOCUS       DATDEU010000689         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_278697, whole genome shotgun sequence.
ACCESSION   DATDEU010000689 DATDEU010000000
VERSION     DATDEU010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278697"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VIV63_16490"
     CDS             complement(<1..494)
                     /locus_tag="VIV63_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126253.1"
                     /translation="MMRLTNPIRLAGVLALVAFAGAAQAEPYLAAQMGLKCVQCHVNP
                     TGGGMRTVFGNAYAQTQLAAKRIGSEEDMWTGQVMKFLSVGGNARANYNFTDVPNQSS
                     TNDFAVEEARAYLDFGVIPNRLSVYIDQRFAPGNSTNLEANIRYWISENSVYVKAGRM
                     YLPFG"
     gene            complement(491..1333)
                     /locus_tag="VIV63_16495"
     CDS             complement(491..1333)
                     /locus_tag="VIV63_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126254.1"
                     /translation="MTIQSNRLLPPKGILGLSVAMMLASCTAGDGTGLDAGGRPLPPA
                     PPANTDFQQIQATIFTPVCTACHVGANAPVGLRLDEGNSYALLVNVASAEVPGLLRVN
                     PGNPDASYLVQKIQGNAAVGARMPANGPPFLSQTQIDLVRRWIAAGAPSSAAPSEQLV
                     VVSSIPAASEQAAAGLGKLTLIFNGDVDSSLANGNAIELRDAFDQPVSLAVRVPTGRQ
                     NVIEVITPQPLAAGSYQLSVRGEGPAPLADQSGHVLDGDADGKPGGDTLIPFDVKAAE
                     GAVR"
     gene            1487..2578
                     /locus_tag="VIV63_16500"
     CDS             1487..2578
                     /locus_tag="VIV63_16500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6126255.1"
                     /translation="MPGIARRQFLARTAAAATLPLFSSLGSAQAPGRKLGVALCGLGS
                     LSTNQIAPALQKTRFCKLAGIVTGSPTKADEWKKKYGLSDNAVYNYQNMAQMASNPDI
                     DIVYVVTPNALHMDHTLAAAAAGKHVFCEKPLEISVERGQRMLDACKKAGKMLGVGYR
                     LQFEPNHLECVRLAREKVLGDVKIIDANFGFAIGEPKQWRLNRALAGGGSLMDVGIYC
                     LQTTRMITGEDPIWINAAEVKTDAVKFKEVDETVLWQAKFPSGVVANSACTYSANGLA
                     GFRAATSRGWFSMDPAYFYNGNRGRRSDGPEINFPVGDQFATELDDFADCIINKRPTK
                     VSGEMGLADVKFLMAIYESIKKGRPVSLA"
BASE COUNT          458 a          826 c          831 g          467 t
ORIGIN      
        1 ccgaacggca ggtacatgcg gccggccttc acgtacacgg agttctcgct gatccagtag
       61 cggatgttcg cttcgagatt cgtggaattg cccggcgcga agcgctggtc gatgtacacc
      121 gataaccggt tgggaatgac gccgaagtcc agataggcac gcgcttcctc gaccgcgaag
      181 tcattcgtgc tgctctggtt gggcacgtcg gtgaagttgt agttggcgcg cgcgttaccg
      241 cccacggaca ggaacttcat gacctggccg gtccacatgt cttcctcgct gccgatacgc
      301 ttcgccgcga gctgggtctg cgcgtacgca ttgccgaaca ccgtgcgcat gccgccgccg
      361 gtgggattca cgtggcactg cacgcacttg aggcccatct gtgcggccag atacggctcg
      421 gcctgcgccg caccggcgaa ggcgacgagc gccagcacgc cggcgagtct gattggattc
      481 gtgaggcgca tcatcggacg gctccttccg cagctttgac atcgaatgga atgagcgtgt
      541 cgccacccgg cttgccgtcc gcgtcgccat cgagcacgtg gccggactga tcggcgagcg
      601 gcgcggggcc ctcgccgcgc acggagagct ggtaactgcc cgcggcgagc ggctgcggcg
      661 tgatcacctc gatcacgttc tgccggccgg tgggaacacg caccgcgagc gacacgggct
      721 gatcgaatgc atcccgcagt tcgatcgcat tgccgttggc gagcgagctg tcgacgtcgc
      781 cattgaagat gagagtgagt ttgccgagtc ccgcggccgc ctgctccgaa gcggcgggaa
      841 tgctgctgac gacgactaac tgttcggacg gagccgcgct cgatggcgcg ccggcggcga
      901 tccagcggcg cacgagatcg atctgcgtct gcgacaggaa cggcggtccg ttggcgggca
      961 tacgcgcgcc gaccgcggca ttgccctgga tcttctgcac tagatagctg gcgtcgggat
     1021 tacccgggtt gacgcgcagg agtccgggca cttcggcgct ggccacgttg accagcaatg
     1081 cataactgtt gccttcatcg agacgcagtc cgacgggcgc attcgcaccg acgtggcagg
     1141 ccgtgcagac cggcgtgaat atcgtcgcct ggatctgctg gaagtcggta ttggcgggtg
     1201 gcgcgggagg cagtggacgc ccgcctgcgt cgagtccggt gccatcaccg gccgtgcagc
     1261 tcgcgagcat catcgccacc gacagcccta aaatcccttt tgggggcagc aacctgtttg
     1321 attggatagt cacggcagtc ccttgttgtg tcgtcgccga agcactccaa actgagtacc
     1381 ggctcgtgcg ttcgcggttc cgtgcgttgc actttggaac gataggtccg gtgcctgggt
     1441 tcggctagac tcgcggtcct tccagaatcc gcctcgagga tcagacatgc ccggaatcgc
     1501 ccgccgccaa ttccttgcac gtacggccgc cgccgccacg ttgccgctgt tcagctccct
     1561 cggcagcgcc caggcgcccg gccgcaagtt aggggtcgcg ctctgcggac tcggcagcct
     1621 gtccaccaac cagatcgctc ccgcactgca gaagacacgc ttctgcaagc tcgcgggcat
     1681 cgtcaccggc tcgcccacga aggccgatga gtggaagaag aagtacggct tgtcggacaa
     1741 cgccgtttac aactatcaga acatggccca gatggcgtcg aacccggaca tcgacatcgt
     1801 ttatgtcgtc actccgaacg cactgcacat ggaccacacg ctggccgccg cggccgctgg
     1861 caaacatgtg ttctgcgaga agccgctgga aatttccgtg gagcgcgggc agcgcatgct
     1921 cgacgcctgc aagaaggccg gcaagatgct gggcgtgggt taccgcctgc agttcgagcc
     1981 caaccatctc gagtgcgtgc gcctcgcgcg cgaaaaagtg ctcggtgacg tcaagatcat
     2041 cgacgccaac ttcggcttcg ccatcggcga gccgaaacag tggcgcctga atcgcgcgct
     2101 ggcgggcggc ggctcgctca tggatgtcgg catctactgc ctgcagacca cgcgcatgat
     2161 cacgggtgaa gatcccatct ggatcaacgc cgccgaggtc aagaccgacg cggtgaagtt
     2221 caaggaagtc gacgaaaccg ttttgtggca ggccaagttc ccgagcggtg tcgtcgcgaa
     2281 ttcggcgtgc acgtattccg ccaatggcct cgccggtttc cgcgccgcga cttcgcgcgg
     2341 ctggttcagc atggacccgg cgtatttcta caatggcaat cgcggccgcc gcagcgacgg
     2401 ccccgagatc aacttcccgg tcggcgacca gttcgcgacc gagctcgacg atttcgcgga
     2461 ctgcatcatc aacaagcggc cgaccaaggt gtcgggcgag atgggcctcg ccgacgtcaa
     2521 gttcctgatg gctatctacg agtcgatcaa gaagggccgt cccgtctcac tcgcgtaaat
     2581 gg
//
LOCUS       DATDEU010000690        11205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_133673, whole genome shotgun sequence.
ACCESSION   DATDEU010000690 DATDEU010000000
VERSION     DATDEU010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133673"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..744)
                     /gene="trmB"
                     /locus_tag="VIV63_16505"
     CDS             complement(34..744)
                     /gene="trmB"
                     /locus_tag="VIV63_16505"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY6126256.1"
                     /translation="MTETPLHNRPIRSFVMRTGRMTVGQTRALEELWPKYGVEFSSQP
                     LSLDSLFGRAAPRTLEIGFGNGEHLAALATAHPERDYFGIEVHRPGVGHLLLLAETNN
                     LTNVRASTHDAVEVLREQIAAESLDEVLVLFPDPWHKKRHHKRRLIQPPFVELIASRL
                     RPGGVLRLATDWEEYALQMLEVLSAARTLFANLSPTGDWMPRPEERAPTRFEKRGARL
                     GHGVWDLAFQKMGSDPIS"
     gene            complement(741..1325)
                     /locus_tag="VIV63_16510"
     CDS             complement(741..1325)
                     /locus_tag="VIV63_16510"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleosidase"
                     /protein_id="HEY6126257.1"
                     /translation="MQPSKLLIVMALPQESRGLLERAGAEVLFTGVGKVNAASALARR
                     LAEMRCTGDGQPLVVNLGTAGSRSIPAHTLVACNRFAQRDIDVSGLGFAPGVTPFDET
                     PAVIEFPVLLPHLAQTLCSTADSFATHRHEVGGDVVDMEAFALARVCRAENARLACAK
                     YVTDGADSDSATHWEAALEAAARSFVTVYEFLAK"
     gene            1354..1427
                     /locus_tag="VIV63_16515"
     tRNA            1354..1427
                     /locus_tag="VIV63_16515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1386..1388,aa:Gly,seq:ccc)
     gene            1528..1800
                     /locus_tag="VIV63_16520"
     CDS             1528..1800
                     /locus_tag="VIV63_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126258.1"
                     /translation="MRYQGDEMASVEVEARWQASDRWSLVGAAGYGSARTERELYSAT
                     RAIVSGAVGFRYEFARKFGLHAGMDVGFSEGTTAVYIQIGNAWFRP"
     gene            1853..2698
                     /locus_tag="VIV63_16525"
     CDS             1853..2698
                     /locus_tag="VIV63_16525"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6126259.1"
                     /translation="MKFRSLAAGVALTAFVATASFAAADKKDVQTIEKAVAAESRSAD
                     SKSRDVYRHPTEALSFWGLKPGMTILEVQPGGGWWTEILAPYAKANKGEFYATAADLA
                     DPKVSEGARKGRADFEAKYANADVYGKVNLVNWGANAAPLPANKFDFVLAARVFHGWA
                     NGGMADTNLAKLYSAIKPGGILAVEQHRGNPGQDPAVKAGYATEQAVIDAATKAGFKL
                     EGKSEVNANAKDTKDHPFGVWTLPPVRQSSEGGKPVDPAFDRAKYDAIGESDRMTLKF
                     VKPKK"
     gene            2718..3296
                     /locus_tag="VIV63_16530"
     CDS             2718..3296
                     /locus_tag="VIV63_16530"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY6126260.1"
                     /translation="MKRLGNILLKGLLTILPIGLTLYFVYWLGVTTETLLSKPLKLVL
                     DDANYWPGMGLITGFVILFLVGLAVNAFVVRRLLGIGEELLLRVPVVKTVYSAIRDMT
                     RLVNTDKKKGDLDRVVTLDFGFGRLIGFVTQEHANTLGIGGGDDLVAVYLPMSYQIGG
                     YTVYLPRSRVVETDLTVEQAMRIVLTGGVRGR"
     gene            complement(3368..4228)
                     /locus_tag="VIV63_16535"
     CDS             complement(3368..4228)
                     /locus_tag="VIV63_16535"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY6126261.1"
                     /translation="MTIAERGEAPFGSFAPRGGHRRLIEFAQRSPHNAIGKQLAHAAR
                     SLYLLRAPSPVDVTVGEIRLRSYLRENTCERKFVFTPWRFDPLELEAMAEALSPEGVF
                     VDVGANVGLYTLTAALLLGEHGRIIALEPQALAHDRLQFNIDATRRGRAGWPRIDVLK
                     LGISDRDESRELRIDRGNLGGGTIASGTARLTDRGAGESVTIRCKPLLQVLDECGVER
                     PDVLKIDIEGAEDLALLPFLAQALDARLPRRLIVENSDHLWKGDLRAAIAGRGYVAQR
                     RSRLNTVYSR"
     gene            complement(4225..5112)
                     /locus_tag="VIV63_16540"
     CDS             complement(4225..5112)
                     /locus_tag="VIV63_16540"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6126262.1"
                     /translation="MNAVIATSQETRATPTIGVVFTTYNRPLDLERVLAGYARQTYGR
                     FEIVIADDGSGEETRACIERARRAWGLNIRHVWQEDIGFRKCRILNRAIVETSAEYLI
                     FTDGDCIPHPEFVAGHLSLARPGYFVSGGCVRLGDSASRAISPPDVIDGKVFDTRWLT
                     AHGESGLNLRKMLLSAPPWHGLMNSLTTTRPTWNGHNSSTWREEVLAVNGFDERLGYG
                     GLDREFGERLERCGMHGIQARYSLICLHLDHPRPYREREIMAANLKIRRDNAALNVRR
                     TRYGLAAQVAMEQRLGTGA"
     gene            complement(5261..5527)
                     /locus_tag="VIV63_16545"
     CDS             complement(5261..5527)
                     /locus_tag="VIV63_16545"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="involved in start site selection during the
                     initiation of translation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HEY6126263.1"
                     /translation="MKPGAPNKPPAIRIWKETAHRGGKGVSAIHGIPLPGPALEEICS
                     QLKKKCGTGGTVKNGVIEIQGDHRDRLVEELKKLGYSDVKKAGG"
     gene            complement(5524..7335)
                     /locus_tag="VIV63_16550"
     CDS             complement(5524..7335)
                     /locus_tag="VIV63_16550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6126264.1"
                     /translation="MTLIALKDAELAFGLTPLLDRASIAIQAGDRVGLIGRNGTGKSS
                     LLNALIGKIPLDAGEVQRRPGLSIVSVEQEPVLPPAETLRESLVLRGDFANMHDDRAL
                     WAREARLNEFLQRFSVNPDASPASVSGGERKRAALALAFAMTPDLMLLDEPTNHLDVE
                     GIETLEELLQRNVTCIVITHDRRFLDKVANSIAELDRGILRSFPGNYSIYEERKAEIN
                     IAEDLAHRRFEKFWAQEEVWIRKGVEARRTRNEGRVKRLEHLREERAARRERIGQIKL
                     TVDTGERSGKLVSELENVGKSFGGRPILDDVSLRVMRGDRVGLLGPNGAGKTTMIKLI
                     VGTLEPDTGRVRRGTNLQVAYFDQLRDQLDPERTLAETVSPGSDWVQVGENRKHISSY
                     LQEFLFDSRRANAPIRMLSGGERNRLLLARLFARTANMLVLDEPTNDLDIDSLELLET
                     MLVDYTGTLLLVSHDRTFLDNVVTQTIASEGNGRWGEYVGGYSDWLRQRSLPRASSNT
                     RASAAPVAAPAAPKRKLSFKEQRELDSLPGEIEKLENEQLALTQKICEPDYHRVGAEQ
                     MRVDRERASEIATLIASRMERWEALEALASQVAGAKA"
     gene            complement(7345..8667)
                     /locus_tag="VIV63_16555"
     CDS             complement(7345..8667)
                     /locus_tag="VIV63_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEY6126265.1"
                     /translation="MNLAAALVAACLLAPASFAQTPAPAKPATNSPTTPKKPSASSSK
                     PKPKPGTSGAKKPARPAAPPPPPPPPAVELIGLIVMPADAMRAGPPAGQFDGEGRRAS
                     APRFDAQPVQGVSSIKPGTTAGAWWALSDNGFGGKWNSSDYRLCIYLFDVRPRTEAGG
                     DSRNALQAVIELSDPAKFFPWRLADENSPERLLTGIDADPESLVVMPDDSFWIGDEFG
                     PWLLHFSADGELLAPPVELPDTVRSADHPLVITHTDIPRLAHSRGLEAMDLAGDNKTL
                     VSILEGTVGGDAADTLRVQRYDTAAGKWIGPTLIYELDQDTTSVTDLSLIEGNRFVVI
                     ERDNFQGDSARAKRVYTIDLDRAVAGKPLQKKLVIDLLVIGNARGLAVTAPGAPFRFP
                     YLTTESIQVLDRKHVVIVNDNNYPAAGGRGNGVKDATEWIWLELANPL"
     gene            complement(8717..10768)
                     /locus_tag="VIV63_16560"
     CDS             complement(8717..10768)
                     /locus_tag="VIV63_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6126266.1"
                     /translation="MTSTIRLGAIALGLTASTTFAAGVPTDDLSEVVVTARSHKLIGH
                     TESSSQGTVTRAQLETRPVLRTGELLETVPGLVVTQHSGDGKANQYFLRGFNLDHGTD
                     LATSVDGIPVNMHTHGHGQGYTDINFVIPELLDRIEYKKGTYYADEGNFSAAGAVDLK
                     YRRDLDDTLLSLSVGEDSYARALFAGSAGLAGGELLFGVDYGVTDGPWDLPENLSRVN
                     ALAKYSRGERGDGYALTASLYDGSWDSTDQIPERAVESGLLDRFGYVDPTDGGESHRY
                     ALNFEIEKPLGEWSLEANAYALDYHLDLFSNFTYALDAANGDQFEQYDDRQAYGVHAE
                     VRRDNLIGSLPGKMRFGFESRFDDIGKVGLYLTEGRERRSTIRQDSVTRLGLGFFAEQ
                     QVQATDWLRVIAGLRFDSAEFDVDSDLDANSGSASDSITSPKISFVFGPWADTEFFIN
                     AGRGFHENDARGTTITVDPADGVTTADRVNPMVRASGAEIGIRTAILPYTQLALTTWM
                     LDLDSELLYVGDAGATEANRASDRHGVELGVYVTPTEWLTLDADLAWSHARFSEPDPA
                     GDYIPGAVEGVASVGVALQHPSGWFGGARFRHFGEAPLIEDNSVRSDPTTVVNLELGY
                     RFAKRFAVSLAAYNLFDSNDNDITYFYESQLPGEGAPVEDRHFHPVEPRTLRLTIETR
                     W"
     gene            complement(10835..>11205)
                     /locus_tag="VIV63_16565"
     CDS             complement(10835..>11205)
                     /locus_tag="VIV63_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126267.1"
                     /translation="GRHRIQGISDEFVPSIVKLGQLGRILDVWDGDAIVMSQRLANEL
                     GIAVGISSGANLLAALEIAHEQGTGACVATVFCDDNKKYLSTDLCSDEECKEHYRCNE
                     VELLDFRVIPACASEGVKRA"
BASE COUNT         1926 a         3536 c         3690 g         2053 t
ORIGIN      
        1 tgacgtattg gtattggggt cagacctcat ttgctaggaa atggggtctg accccatttt
       61 ctggaaggcg aggtcccaca cgccgtggcc gaggcgggcg ccgcgtttct cgaaacgcgt
      121 gggagcgcgt tcctcggggc gaggcatcca gtcgccggtg ggcgagaggt tggcgaacag
      181 ggtcctggcc gcgctcagta cttccagcat ctgcagcgcg tactcttccc agtcggtagc
      241 tagtctgagg actccacccg gccgcagtcg cgaggcgatc aattccacga acggcggctg
      301 gatgagccgg cgtttgtggt gacgcttctt gtgccaggga tcggggaaca ggacgagcac
      361 ctcgtccagt gattccgcgg cgatctgctc gcgcaacact tcgacggcat cgtgcgtgct
      421 cgcgcgcacg ttcgtgaggt tgttagtctc cgccagcagc aacaggtggc cgacaccggg
      481 gcgatgcacc tcgatgccga agtaatcgcg ctccggatgc gccgtcgcga gcgcggcgag
      541 atgttcgcca ttgccaaagc cgatctccag cgtgcgcggc gcggcgcggc cgaacagcga
      601 gtccagcgac aagggctgcg atgaaaactc cacgccgtat ttcggccaga gctcctcgag
      661 cgcgcgcgtc tggcccaccg tcatgcggcc ggtgcgcatc acgaagctgc ggatggggcg
      721 attgtgaagc ggggtttcgg tcatttcgca aggaactcgt agacggtgac aaagctgcgc
      781 gccgcggcct cgagcgcagc ctcccagtgt gtcgccgaat cgctgtcggc gccatcggtg
      841 acgtacttcg cgcaggcgag gcgcgcgttt tcggcgcggc acacgcgcgc gagggcgaag
      901 gcttccatgt cgaccacgtc gccgcccact tcgtgacggt gcgtcgcgaa ggaatccgcc
      961 gtcgaacaca aagtctgcgc aaggtgaggc agcagaaccg gaaactcgat gacggccggc
     1021 gtctcgtcga acggtgtgac acccggcgca aatccgagac cgctcacatc tatgtcccgt
     1081 tgcgcgaagc gattgcacgc caccagcgta tgcgcgggaa tcgagcggct gcccgccgtc
     1141 cccaggttca ccaccagcgg ctgtccgtcg cccgtgcaac gcatctcggc caatcggcgc
     1201 gcgagcgccg atgctgcgtt taccttgccg accccggtga acaggacttc ggcgccggcg
     1261 cgttccagca ggccgcggga ttcctgcggc aacgccatga cgatcaacag tttcgaaggc
     1321 tgcatgtcgc ctgttatcct ttcggccccg agcgcgggtg tagttcaatg gtagaactgt
     1381 agcttcccaa gctactaacg tgggttcgat tcccatcacc cgctccactt ccgttcgtgc
     1441 gagaacgtct gtgggtcgat gcatgctcac tggtttgaac cgcgcaggaa tccgcgccca
     1501 ccgctgccgc ggcgcggcgt cgaggccatg cgttaccagg gcgatgagat ggcctccgtc
     1561 gaagtggagg cgcgctggca ggcctccgac cgatggagtc tcgtcggcgc ggccgggtat
     1621 ggcagcgcgc gtaccgagcg cgagttgtat tcggccacgc gcgccatcgt gagcggcgcc
     1681 gtcggcttcc gttacgaatt cgcacgcaag ttcggcctgc atgcgggcat ggatgtggga
     1741 ttcagcgagg gtacgactgc agtctatatc cagatcggca acgcctggtt caggccatag
     1801 gagaaagcct gtaggattcc tgccccgaaa tttgaatcga gagggatcct ccatgaaatt
     1861 cagatcactt gccgccggcg tggctctcac agccttcgtc gcgaccgcga gctttgccgc
     1921 ggccgacaaa aaggatgtcc agaccatcga aaaagccgtg gctgccgaat cgcgcagcgc
     1981 cgactccaag tcgcgcgatg tgtaccgtca tcccaccgaa gccctctcgt tctggggcct
     2041 gaagcccggc atgacgatcc tcgaagttca gcccggcggt ggctggtgga ccgagatcct
     2101 cgcgccctac gcgaaagcga acaaaggcga gttctacgcc acggccgccg atctcgccga
     2161 ccccaaggtt tccgaaggcg cgcgcaaagg gcgtgcggac ttcgaagcca agtacgcgaa
     2221 cgccgacgtg tacggcaagg tgaacctggt gaactggggc gcgaacgccg cgccgctgcc
     2281 cgccaacaag ttcgacttcg tgctcgccgc gcgtgtgttc cacggctggg cgaatggcgg
     2341 catggccgac accaacctcg cgaagctgta cagcgccatc aagccgggtg gaatcctggc
     2401 cgtcgaacag catcgcggta acccgggcca ggacccggcc gtgaaggcgg gctacgcgac
     2461 cgagcaggcc gtgatcgacg ccgccaccaa ggcgggcttc aagctcgaag gcaagtcaga
     2521 ggtcaacgcg aacgcgaagg acacgaagga ccatccgttc ggcgtgtgga cgctgccgcc
     2581 ggtgcgccag tcttccgaag gcggcaagcc cgtcgacccg gcattcgatc gcgccaagta
     2641 cgacgcgatc ggcgagtcgg atcgcatgac gctcaagttc gtcaagccga agaaataacc
     2701 tggacgaagt caccgcgatg aagcgtctcg gaaacatcct gctgaagggc ctgctgacca
     2761 tcctgccgat cggtctcacg ctctatttcg tctattggct gggtgtcacc accgagacgc
     2821 ttctgtcgaa gccgctcaag ctggtgctcg acgacgctaa ctactggccg ggcatggggc
     2881 tcatcacggg cttcgtgatc ctgttcctgg tcggtctcgc ggtgaacgcc ttcgtggttc
     2941 gccgtctgct cggcatcggc gaagagctgc tgctgcgcgt gccggtggtc aagacggtgt
     3001 acagcgccat ccgcgacatg acgcggctgg tgaacaccga caagaagaag ggcgatctcg
     3061 accgcgtggt cacgctcgac ttcggcttcg gccggctcat cggcttcgtg acccaggaac
     3121 atgccaatac gctcgggata ggcggcggcg acgacctcgt cgccgtctat ctgcccatga
     3181 gctaccagat cggcggatac acggtctatc tgcctcgcag ccgcgtggtc gagaccgacc
     3241 tcactgtcga gcaggcgatg cgcatcgtgc tgaccggcgg cgtccgcggc cgttaatggg
     3301 gacatggatg tgtggggact gacttaagtc tgtccccact cgtttagcta gcaaatccgg
     3361 acgcgcctca gcgcgagtag accgtgttca ggcgcgagcg acgctgcgca acgtagccgc
     3421 gccccgcaat cgccgcgcgc aggtcgcctt tccacaagtg gtcggagttc tcgacgataa
     3481 gccggcgcgg caggcgcgca tcgagtgcct gcgccagaaa tggcagcagc gccaggtctt
     3541 cggcaccctc gatgtctatc ttcagcacat cgggtcgttc gactccgcac tcatccagca
     3601 cctgcagcag cggcttgcag cggatcgtca ccgattcgcc cgctccgcga tccgtcaggc
     3661 gcgcggttcc gctggcgatg gtgccgccac ccagattgcc gcggtcgatc cgcaactcgc
     3721 gcgactcatc gcgatctgaa atgccgagct tcaggacatc gatgcgcggc cagccagcgc
     3781 ggccgcgacg cgtggcgtcg atgttgaatt gcagccggtc gtgcgcgagc gcctggggtt
     3841 cgagcgcgat gatcctgccg tgttcaccta acaatagcgc ggccgtcaac gtgtagaggc
     3901 cgacattagc gccgacgtcg acgaacacgc cctcgggtga cagcgcctcg gccatcgcct
     3961 cgagctccag cggatcgaag cgccacggcg tgaagacgaa cttccgctcg caggtgttct
     4021 cgcgcaggta actgcgcagc cggatctcgc cgaccgtgac atccacgggc gatggtgcgc
     4081 gcagcagata gagactccga gccgcgtgcg ccagttgttt gccgatcgcg ttgtgcggcg
     4141 atcgttgtgc gaattcgatg agccgccggt ggccgccgcg cggggcgaag ctgccgaagg
     4201 gcgcttcgcc tctctcggcg atcgtcacgc gcccgtgccg aggcgctgtt ccatggctac
     4261 ctgcgccgcc aggccatagc gcgtgcgccg cacattgagc gcggcgttgt cgcggcggat
     4321 cttgagattc gccgccatga tctcgcgctc gcgatacggc cgcggatgat cgagatgcag
     4381 gcagatcagc gagtaacgcg cctggatgcc gtgcatgccg catcgttcca ggcgttcgcc
     4441 gaattcacga tcgaggccgc cgtagccgag ccgctcatcg aagccgttca cggccagtac
     4501 ttcttcgcgc cacgtcgatg agttgtgacc gttccacgtc gggcgcgtcg tggtcagcga
     4561 attcatgagt ccatgccatg gcggcgccga cagcagcatc ttgcgcaggt tgagaccgga
     4621 ctcgccgtgc gcggtgagcc agcgtgtatc gaacaccttg ccgtcgatca cgtctggcgg
     4681 cgaaatcgcg cgcgatgcgg aatcgcccag gcgcacgcat ccgcccgaca cgaagtatcc
     4741 cggacgcgcg aggctcaagt gcccggccac gaattccgga tgcggaatgc agtcgccgtc
     4801 cgtgaagatc aaatactccg cagaagtttc gacaatcgcg cgattgagga tccggcattt
     4861 gcgaaagccg atgtcttcct gccacacgtg gcggatgttg agtccccagg cccggcgcgc
     4921 gcgttcgatg cacgcgcgcg tctcttcgcc ggatccgtcg tcggcgatga cgatctcgaa
     4981 gcgaccatag gtctgacgcg cgtaacccgc gagcactcgc tccaggtcca gcggacggtt
     5041 gtaggtggtg aacaccacgc caatcgtggg tgtggcgcgg gtttcttgcg aagtggcaat
     5101 gacggcattc atgcgccgat ttcgccagcc gaatgtttcg gttccgctac ggttttgtgt
     5161 cggatttccg acgaggcgtc agcccgcgag gcgcgtccgc ttttgctagc taaacgagtg
     5221 gggacagact taagtcagtc cccacacacc catgtcccca ttacccgccg gccttcttca
     5281 cgtcgctgta gccgagtttc ttcagctctt cgacgaggcg gtcgcgatga tcaccctgga
     5341 tctcgatcac gccgttcttc acggtgccgc cggtgccgca cttcttcttg agctggctgc
     5401 agatctcttc gagcgccggc ccgggcagcg gaataccgtg gatcgcgctc acgcccttgc
     5461 cgcctcgatg cgcggtttcc ttccagatgc ggatcgccgg tggtttgttg ggcgcgcccg
     5521 gcttcatgcc ttcgcacccg ccacctgcga tgccagcgcc tcgagtgcct cccagcgttc
     5581 catgcgcgag gcgatgagcg tcgcgatctc gctcgcgcgc tcgcgatcga cgcgcatctg
     5641 ctcggcgccg acgcggtgat agtccggctc gcagatcttc tgcgtcagcg cgagctgttc
     5701 gttctccagc ttctcgattt cgccgggcag cgagtcgagc tcacgctgct ccttgaagct
     5761 gagtttgcgc ttgggtgcag ccggcgccgc caccggcgcg gccgatgcgc gcgtgttgct
     5821 ggacgctcgc ggtaacgaac gctgccgcag ccagtcgctg tagccgccca catattcgcc
     5881 ccagcggccg ttgccttcgg aagcgatggt ctgcgtgacc acgttgtcga ggaacgtgcg
     5941 gtcgtggctc acgagcagca gcgtgccggt gtaatcgacg agcatggtct cgagcagttc
     6001 gagcgagtcg atgtcgagat cgttggtcgg ctcgtcgagc accagcatgt tggccgtccg
     6061 cgcgaacagg cgcgccagca gcaggcgatt ccgttcgccg cccgaaagca tgcggatggg
     6121 cgcattggcg cggcggctgt cgaacaggaa ctcctgcagg tagctggaga tgtgtttgcg
     6181 gttctcgccg acctgcaccc agtcggagcc ggggctcacg gtttccgcga gcgtgcgttc
     6241 ggggtcgagc tgatcgcgca gctggtcgaa gtaggccacc tgcaggttgg taccgcgccg
     6301 cacgcgcccc gtgtccggct cgagcgtgcc gacgatgagc ttgatcatgg tggtcttgcc
     6361 ggcgccgttg gggcctaaca agccgacgcg gtcgccgcgc atcacgcgca ggctgacgtc
     6421 gtcgagaatc ggccggccgc cgaagctctt gccgacattc tcgagttccg aaaccagttt
     6481 gccggagcgc tcgccggtat ccacggtgag tttgatctgg ccgatgcgct cgcgtctcgc
     6541 ggcgcgctct tcgcgcaggt gctcgagccg cttcacgcgg ccctcattgc gcgtgcggcg
     6601 cgcctcgaca cccttgcgga tccacacttc ttcctgcgcc cagaatttct cgaagcgccg
     6661 gtgcgcgaga tcctcggcga tgttgatttc cgccttgcgc tcttcgtaga tggaatagtt
     6721 gcccggaaag ctgcgcagga tgccgcgatc gagctcggcg atggagttgg ccaccttgtc
     6781 gaggaagcgc cggtcgtgcg tgatcacgat gcaggtgaca ttccgctgca gcaactcttc
     6841 gagcgtctcg atgccttcga cgtcgagatg gttcgtcggc tcgtcgagca gcatcaggtc
     6901 cggcgtcatc gcaaacgcca gcgcgagcgc ggcgcgtttg cgctcgccgc ccgagaccga
     6961 agcgggcgac gcgtccggat tcaccgagaa gcgctgcagg aattcgttga gccgcgcttc
     7021 gcgggcccac aacgcgcggt cgtcgtgcat gttcgcgaaa tcaccgcgca gcaccagcga
     7081 ctcgcgcagc gtctcggccg gcggcagaac cggttcctgt tcgacgctga cgatgctcag
     7141 gcccggccgg cgctgcactt cgccggcgtc gaggggaatc tttccgatga gcgcgttcaa
     7201 caacgacgac ttgccggtgc cgttgcggcc gatgagcccg acgcggtcgc ccgcctggat
     7261 ggcgatcgaa gcgcggtcga gaaggggagt gagtccgaac gcgagttccg cgtccttgag
     7321 ggcgatcagt gtcacgacat caacttataa cgggttggcg agctcgagcc agatccactc
     7381 ggtggcgtct ttcacgccat tgcctcggcc gccggcggcc gggtaattgt tgtcgttcac
     7441 gatgacgacg tgcttgcggt cgagcacctg gatggattcg gtggtcagat acggaaagcg
     7501 gaagggagcg cccggcgccg tcaccgcgag gccacgcgca ttgccgatga ccagaagatc
     7561 gatgaccagc ttcttctgca acggctttcc ggccaccgcg cgatccaggt cgatggtgta
     7621 cacgcgcttg gcgcgcgccg agtcaccctg gaagttgtcg cgttcgatga ccacgaaacg
     7681 attgccctcg atcagcgaca ggtcggtgac cgacgtggtg tcctggtcga gctcatatat
     7741 aagagtgggg ccgatccact ttccagccgc cgtgtcatac cgctgcacgc gcagcgtgtc
     7801 tgctgcatcg ccgccgaccg tgccttcgag aatggacacc agcgtcttgt tgtcgccggc
     7861 gagatccatg gcttcgaggc cccgggaatg cgcgaggcgg ggtatgtcgg tgtgggtgat
     7921 gaccagtgga tggtcggccg agcgcacagt gtccggcagc tcgaccggcg gggctaacaa
     7981 ctcgccgtcg gcggagaagt gcagcaacca cgggccgaac tcgtcgccga tccagaagga
     8041 gtcgtcgggc atgaccacca gcgactcggg gtccgcgtcg atgccggtga gcaaacgttc
     8101 gggcgagttc tcatccgcga ggcgccacgg gaagaatttc gccggatccg aaagctcgat
     8161 cacggcctgc aacgcgttgc gcgagtcgcc gccggcctcg gtgcgcggac gcacgtcgaa
     8221 tagatagatg cagaggcggt agtcggagct gttccacttg ccaccgaagc cgttgtccga
     8281 cagggcccac cacgcgccgg cggtggtgcc gggtttgatg gagctcacgc cctggacggg
     8341 ctgggcatcg aagcgcggag ccgaagcgcg gcggccctcg ccgtcgaact gccctgccgg
     8401 cggaccggcg cgcatggcgt cggcgggcat gacgatgagg ccgatgagtt cgactgccgg
     8461 cggtggcggg ggcggcggtg gagccgcggg ccgggccggt ttcttcgcac ccgaggtccc
     8521 aggcttcggc ttgggtttcg aactggaagc cgaaggtttc ttgggcgtgg tgggcgagtt
     8581 agttgccggc ttggccggcg cgggtgtttg cgcgaaggaa gccggcgcaa gcaggcatgc
     8641 cgcaaccagg gcggcggcca ggttcaaggc gcgcgaagac acagcaagca tggccgcgga
     8701 ttctactcac aaacatctac caacgcgttt cgatggtcag gcgcagcgtg cgcggctcca
     8761 ccggatggaa atggcggtct tcgacgggcg caccttctcc gggcaactgg ctttcgtaga
     8821 aatatgtgat gtcgttgtcg ttcgaatcaa acaggttgta ggccgcgagc gagacggcga
     8881 agcgtttggc gaagcgatag cccagctcca gattgaccac cgtggtgggg tcggagcgca
     8941 cgctgttgtc ttcgatgagc ggcgcctcac cgaaatgacg gaatcgtgcg ccaccaaacc
     9001 atccactcgg atgctgaagc gcgacgccga cggaggccac gccttccact gcgcccggga
     9061 tgtagtcgcc ggccggatcg ggctcactga agcgtgcgtg cgaccacgcc aggtccgcat
     9121 cgagcgtaag ccattcggtc ggagtgacat acacaccgag ctcgacacca tgtcgatcac
     9181 tggcgcgatt cgcttcggtc gcgccggcat ccccgacgta gagcagttct gaatcgagat
     9241 ccagcatcca ggtggttagt gcgagttgtg tgtacggcag gatagccgta cgaattccaa
     9301 tctctgcccc gctcgcgcgg accatgggat tgacgcgatc ggcagtggtc acgccgtcgg
     9361 ccggatccac ggtaatggtc gtgccccgcg cgtcgttctc gtgaaaaccg cgaccggcgt
     9421 tgatgaagaa ttccgtgtcg gcccacgggc cgaacacgaa ggaaatcttg gggctggtga
     9481 tgctgtcgct ggcggagccg ctgttcgcat cgagatcgct gtcgacgtcg aactcggcgc
     9541 tgtcgaatcg caaacccgcg atcacgcgca gccagtcggt ggcctgcacc tgctgttcgg
     9601 cgaagaagcc gaggcccagg cgcgtcacgg aatcctgtcg gatcgtcgag cggcgctcac
     9661 ggccttcggt gagatagagg ccgaccttgc cgatgtcatc gaagcgcgat tcgaaaccga
     9721 aacgcatttt tcccggcagg ctgccgatga ggttgtcgcg gcggacttca gcgtgcacgc
     9781 catacgcctg gcgatcatcg tactgctcga actggtcgcc gttcgcggcg tcgagggcgt
     9841 acgtgaagtt cgagaacaga tcgaggtgat agtcgagtgc gtacgcattc gcttcgaggc
     9901 tccactctcc gagcggcttt tcgatctcga agttcagcgc gtagcggtgc gattcgccac
     9961 cgtcggtggg atcgacgtaa ccgaaccggt ccaacaaacc cgattcgacc gcgcgctccg
    10021 ggatctggtc ggtcgagtcc cagctgccgt cgtagaggga agcggtaagc gcgtaaccgt
    10081 cgccacgctc gccgcgcgag tacttcgcga gcgcgttcac gcggctcagg ttctccggca
    10141 gatcccaggg gccgtcggtg actccgtagt cgacaccgaa gagaagctcg ccaccggcga
    10201 ggcccgccga gcccgcgaac aaggcgcgcg cataggagtc ttcaccgacg ctgagcgaca
    10261 acaacgtgtc gtcgagatcg cgtcgatatt tgagatcgac ggcgccagcc gcggagaaat
    10321 tgccttcgtc cgcgtagtac gtgcctttct tgtattcgat gcggtcgagc agttcgggaa
    10381 tcacgaaatt gatgtcggtg tagccctggc cgtggccatg cgtgtgcatg ttcacgggga
    10441 ttccatcgac gctggtggcg aggtcggtgc cgtgatccag gttgaagccg cgcaggaaat
    10501 actgattcgc cttgccgtcg ccgctgtgtt gcgtgacgac gagtccggga acggtttcca
    10561 gcaactctcc ggtgcgcaac accggacgtg tttcgagctg cgcgcgtgta accgtgccct
    10621 ggctcgagga ttccgtatgg ccgatgagtt tgtggcttct tgccgtgacc acgacctcgg
    10681 aaagatcgtc agtgggaacg ccggcggcaa aagtggtgga tgccgtcaaa ccgagcgcaa
    10741 tggcgcccag gcgaatcgtc gatgtcattt ttgttacccc gaagcggccc atagcctagc
    10801 ccggcatgtg ttgcggtttt attgcccgca actgttacgc gcgcttgacg ccctcggatg
    10861 cacacgccgg gatgacgcgg aagtcgagca gctcgacctc gttgcagcgg tagtgctcct
    10921 tgcactcctc gtcgctgcac aggtcggtcg acagatattt cttgttgtcg tcgcagaaca
    10981 cggtagccac gcaggcgccc gtgccttgtt catgcgcgat ctccagcgcc gcgagcaggt
    11041 tggcgccgga gctgatgccg acggcgatgc cgagttcgtt cgcgaggcgc tgcgacatga
    11101 cgatggcgtc gccatcccag acgtcgagga tgcgtccgag ctgaccgagc ttgacgatgg
    11161 atggcacgaa ctcgtcgctg atgccctgga tgcgatgacg cccca
//
LOCUS       DATDEU010000691         8273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_133753, whole genome shotgun sequence.
ACCESSION   DATDEU010000691 DATDEU010000000
VERSION     DATDEU010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133753"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VIV63_16570"
     CDS             <1..1257
                     /locus_tag="VIV63_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126268.1"
                     /translation="FFEDPTEQSLLHYLQSKFKEIQDLDAAERHALGALSEAAERSGV
                     RGEFPMYHDVEREHRAQMTGERLETVTAFLKFFEREEAHAASSARMDAGHGAAEFDSS
                     NPHISRIAYEARTWRERAPDDMERFTEILAGIRPGRMRLLPAILANVRKSTAANAITA
                     VHLQKLVAALRNRASPVRPKCQYIKAGNPVRYAWDDRRSRFIARNSRTPSALSLQLLG
                     PSDTLVNRFRDRLPVGTYAEALAFRREPIPRESITASNQLSYILVLEAHEQRVLITGD
                     AGCYDFRDDTGTYYSSLIRALSPLHVVQIAHHAGHNYDFYNCLLEADLPAQREQSFLL
                     LSHDYHDVSRPSDAFRRFMAQMRADGDDLTLLFTSEPTLAKVRDYDELIAPLVPSGSS
                     KKSGDARLVYGGRRAEWSVARHVVAV"
     gene            complement(1309..1668)
                     /locus_tag="VIV63_16575"
     CDS             complement(1309..1668)
                     /locus_tag="VIV63_16575"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY6126269.1"
                     /translation="MTTPSGHSEVIRAKKVIGTSVKNTAGQKIGQIEDIVLDKLSNNI
                     VYAVVGFGGFLGVNEKFHPVPWSALDYVESEDSYVVPFTKEELNAAPADSLEKLTRGD
                     GAAAYSKSANAYYKVNH"
     gene            1868..3181
                     /locus_tag="VIV63_16580"
     CDS             1868..3181
                     /locus_tag="VIV63_16580"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY6126270.1"
                     /translation="MLSNETSSIRLLELVRDPLLDAVTALASARDETAVCVIVRRAAR
                     ELTEADGVTFVLRRGNYCYYADEDAIGPLWKGKQFPIETCISGWAMINRQHVVVPDIY
                     SDDRIPHDAYRPTFVKSLAMVPVRREDPVAAIGAYWATYHHATPAEIGVLQSLADAAA
                     LALYNVQLDANLKEAVRQERDAREAAESASRLKDEFLALLSHELRTPLHVINNWLWQL
                     KQGKSVSPMILSRALDVIERNTTLQTRLIDDLLDVSRAAAGKLSIDSQLVQLGTLAAA
                     VVQNNQPAANEKSIALTLRQDDNPTVWGDPERLQQVMNNVVSNAIKFSSKDGQVEVRV
                     GRDGTLASVVVKDNGPGIAADFLPHMFERFRQAESTMTRRQGGLGLGLTIVRDIMALH
                     GGTVRAESAGKDLGTTIHLEFAVAEEPQAGSEVLNQASRSNQPVH"
     gene            3182..3544
                     /locus_tag="VIV63_16585"
     CDS             3182..3544
                     /locus_tag="VIV63_16585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6126271.1"
                     /translation="MKATKVLIAEDYDDARDVMAMVLQSAGFQVFEARDGVEALEAAR
                     NFHPAIIVMDMFMPNMDGLTATRALRDEPDLRRIPVIAQTAQPSVISGSRELFDAVLA
                     KPCSPDILIRTISELTTR"
     gene            complement(3592..4713)
                     /locus_tag="VIV63_16590"
     CDS             complement(3592..4713)
                     /locus_tag="VIV63_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY6126272.1"
                     /translation="MTIPAANQGRFGAAFEWLGVERAWNRTRGSADVVIGIVDEGVQV
                     DHPLLGPNIQRDLAHNLAAAGSHEIAGTHAAGVAAGRFSEVERFSGVAPDARILPVRF
                     TNGTGPQALDLAHAIEYAVEMGACIINVSPAGEVGTPAVQRAIQYAAARNVLVVCSAS
                     DASSPMALAADDRVPNQINVLSVGEQVQPLAQCARQAAHLAAPGFARVPQWRGSGHSV
                     LLGNAIGAAYVSGCAALVKAINPAWGYHEIKEHLLASGTVYPSLEGRCQTGAVINVAN
                     AVLGPIELADGAQSLTWTSLNDAVLEWKLRYRAALCANAVALYRPHGDDHWRELACAR
                     AGTLKMTIPGKALRRSTGTLRIACRESNFYSDEIALTIA"
     gene            complement(4794..5423)
                     /locus_tag="VIV63_16595"
     CDS             complement(4794..5423)
                     /locus_tag="VIV63_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126273.1"
                     /translation="MNQELIEQYVAGRLSEPDAQAFEDYCVANPEFARQVEYEQRLKA
                     GIGTIARGSTAEFVRAPHPLRWTLAAAASVLVAIVAMFYLWNPGSRIAAPQILAAFTD
                     SSPRDGTSLRLALVRGSDTAPTLPPGMVRVEIVGLFDVEHQYSVALDRLEQNKKIDTV
                     ATLNDQRPNSPVSLIVVIDSKQLRPGPYSLRVRKQASGEEALDFGFVKF"
     gene            complement(5420..6013)
                     /locus_tag="VIV63_16600"
     CDS             complement(5420..6013)
                     /locus_tag="VIV63_16600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6126274.1"
                     /translation="MDFVPGGELSRRIRQGDISAESELIRQFEPGLRVLLRRRTGGDH
                     GLLQDLVQETLLVVIQRLRGEGIDDPQKLAAFAAQTARNLAIASLRKAERQRTDVDSE
                     ATERNADPTQSVDRIASDLEAALAVRALLRELPQPRDRLMLKRFYLDDHDKELICQEL
                     DLSEAAFNQALSRARRRFRQILEERGFAKRDLLSLGL"
     gene            6127..6528
                     /locus_tag="VIV63_16605"
     CDS             6127..6528
                     /locus_tag="VIV63_16605"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6126275.1"
                     /translation="MLKTYHGSCHCGAVRFEADIDLTQPTYRCNCSICSRNRFWAAVV
                     KPGAFRLLEGKSAVQQYLFNSMQNEHFFCRTCGVRAYGLGNSPDVGEMVGVNIGCLED
                     TTPEELAAAPIRYCDGRYDNWQNEPAITSYL"
     gene            complement(6529..7791)
                     /locus_tag="VIV63_16610"
     CDS             complement(6529..7791)
                     /locus_tag="VIV63_16610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6126276.1"
                     /translation="MNSLPQHMQTEPEEDGQSASAERRNLADALAHERNVLRTMIDLI
                     PAFIYAKDAQSRFTAANKLVANRMGVEPSDLIGKTDFDFFPREHAERFFADEQTLIQS
                     GIPLINREEIAFDKTRGMDRIILTSKIPLRDAAGNLTGIVGTGIDITDRKDAEERMAS
                     SDRLESIGRLAAGVAHEINTPIQYLNDSVTFIRDGVGELLAYIDELHKATETRPATSE
                     DVDYMREELPPALTRVVEGLSRITEIVRSMKDFSHADQREMSDVDLNRAITSTLVVAR
                     SEYRDVADVETDLGEIPRVTCHGGQINQVVLNLVVNAAHAIGDKIKGTGGRGKIVVST
                     RTENDQVVISIADTGGGIPENIRPRIFDPFFTTKEVGRGTGQGLSVSRNVVVKGHGGE
                     LDFVTEPGKGTTFFVRLPVAGLPPADAG"
     gene            complement(7917..>8273)
                     /locus_tag="VIV63_16615"
     CDS             complement(7917..>8273)
                     /locus_tag="VIV63_16615"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEY6126277.1"
                     /translation="YSALYDQAGLMVRLDEKHWMKCGSEFVENKRWASVVFTHDFSDW
                     STMEDVSQTGAAWWRVVRRKDSIEAQCSKDGEKFTTIRQGYFPADVQVMAGVMCAAPE
                     GAGFDAVFDQLSIEKA"
BASE COUNT         1528 a         2556 c         2612 g         1577 t
ORIGIN      
        1 tttttcgaag atcccaccga acaatcgctg ttgcactacc tgcagagcaa gtttaaggaa
       61 atccaggatc tggacgcggc cgagcgacac gcgctcggag cgttgtcgga agcggcggaa
      121 cgcagcggtg ttcgcggtga atttccgatg tatcacgacg tcgagaggga gcatcgtgcc
      181 cagatgaccg gtgagcggtt ggagaccgtt acggcgttcc tgaaattctt cgaacgagag
      241 gaagcgcatg ccgcaagcag cgcgcgcatg gacgcgggac acggcgccgc cgaattcgat
      301 tcgagcaacc cgcacatcag ccggattgcg tacgaggcgc gaacctggcg tgaacgggcg
      361 cccgatgaca tggagagatt cacggagatt ctggccggga tccggccagg cagaatgcgc
      421 ctcttgccgg cgattctcgc caacgtccgc aaatcgacgg ccgcgaatgc gataacggcg
      481 gtccacctgc agaagctcgt ggccgcgctg cgaaatcgtg cgtctccggt gcgtccgaag
      541 tgccagtaca tcaaggcagg aaatccggtg cggtatgcat gggacgatcg ccgttcccgt
      601 ttcatcgcac gcaacagccg gacgccgtcg gctctgtcac tgcaattgtt aggcccgtcg
      661 gatactttgg tgaatcgatt ccgtgaccgg ctgcccgtgg gcacatatgc cgaagcgctg
      721 gcgttcaggc gcgagcctat tccgcgggag tcgatcaccg cgagcaatca gctcagttac
      781 atcctggtgc tcgaggcgca cgaacaacgc gtgctgatca ccggcgatgc cggctgttat
      841 gactttcgcg acgacaccgg gacttactac tcaagtctga tcagggcact ctcgccgttg
      901 catgtcgtac agatcgcgca ccatgccggg cacaactatg atttttacaa ttgcctgctg
      961 gaagctgact tgcctgccca gcgcgagcag tcgttcctgt tgctgtcgca cgattatcac
     1021 gatgtatcgc gcccatcgga tgcgttccgt cgtttcatgg cgcagatgcg cgcggacgga
     1081 gatgacctga cacttctgtt cacgtcagag cccacgcttg cgaaagtgcg cgactacgac
     1141 gaactcatcg cgccgctggt tccgtcgggt tcaagtaaaa aatctggcga cgccaggttg
     1201 gtgtacggcg gacgccgggc ggaatggagc gtcgcccgtc acgtggtagc ggtctgaaac
     1261 gccgctagcg ggcaacggcc gtttcggccg tgcccaccgt gcgatcgttt agtggttgac
     1321 cttgtagtac gcgttcgcgc tcttgctgta cgcggcggcg ccatcgccac gcgtcagttt
     1381 ctcgagcgaa tccgccgggg cggcattcag ctcttccttc gtgaacggta cgacgtagct
     1441 gtcttcgctt tcgacgtagt cgagcgcgga ccacggcacc gggtggaact tctcgttgac
     1501 accgaggaat ccgccgaagc cgacgacggc gtagacgatg ttgttcgaaa gcttgtcgag
     1561 cacgatgtct tcgatctggc caattttctg gcccgccgtg ttcttgacgc tggtgccgat
     1621 caccttcttc gcgcgaatga cttcagagtg gcctgatggg gtagtcatgt gatgctccat
     1681 gtgttcgttt gaatgcgcgg cgatggtaag gatcgcggcg cctgcgcccc atcagcccat
     1741 gtcgcgcccg ggtgtcagac ctgacgcggc tgggcaagcg gaccttcatg taggaatcca
     1801 tgagcggctc agccaaaggt aggagggggc tcaccgattg ctaccgcgct gcgacatggg
     1861 catcctgatg ctgtccaacg aaacctcgag cattagactt ttggagctgg tccgcgatcc
     1921 ccttctggat gccgtcaccg cactcgcgtc cgcgcgggat gaaaccgctg tctgcgtcat
     1981 cgtccggcgg gccgcacggg aactcaccga ggccgacggc gttaccttcg tgctccgccg
     2041 cggaaactac tgctattacg cggatgagga cgccatcggt ccgctctgga agggcaaaca
     2101 gtttccgatc gaaacctgca tttcgggctg ggcgatgatc aaccgccagc acgtggtggt
     2161 gccggacatc tactccgacg accgcatccc gcacgatgcc tatcgaccca ccttcgtgaa
     2221 gagcctggcg atggtgccgg tgcggcgtga agacccggtc gcggcgatcg gcgcttactg
     2281 ggcgacttat caccacgcca ctcccgcgga aatcggtgtg ctccagagcc tggccgatgc
     2341 ggcggcgctg gcgctgtaca acgtgcagct cgatgcgaat ctgaaggaag ccgtgcgcca
     2401 ggagcgtgac gcgcgtgaag ccgccgagag cgcgagccgg ctcaaggacg aattcctggc
     2461 gttgttgtca cacgaattgc gtacgccgct gcacgtcatc aacaactggc tctggcagct
     2521 caaacaaggc aagtcggtgt cgccgatgat cctgtcgcgg gcgctggacg tgatcgagcg
     2581 caacaccacg ttgcaaacac gcctgatcga cgacctgctc gatgtgtcgc gcgcggcggc
     2641 gggcaaattg tcgatcgact cgcagctcgt gcaactcggc acgctggcgg ccgcggtcgt
     2701 gcagaacaac cagccggcgg cgaacgagaa atcgattgcg ctcacgctca ggcaggacga
     2761 caacccgacc gtctggggcg acccggaacg gttgcagcag gtcatgaaca acgtcgtttc
     2821 caacgccatc aagttttcgt cgaaggacgg gcaggtcgag gttcgcgtgg ggcgcgacgg
     2881 aaccctcgca agcgtggtcg tgaaggacaa cgggccgggc atcgcggcgg acttcctgcc
     2941 gcacatgttc gagcgttttc gccaggccga atccacgatg acacgccggc agggcggcct
     3001 cgggttgggg ctgacgatcg tgcgagacat catggccctg cacggcggaa cggtgcgcgc
     3061 ggagagcgcg ggcaaggacc ttggaaccac gattcacctg gaattcgccg tcgccgaaga
     3121 gccgcaagcc ggcagcgaag tgttgaacca ggcgtcgcga tcgaatcagc cggtgcattg
     3181 aatgaaggcg acgaaagtcc tgatcgcgga agattacgac gacgcgcgcg atgtcatggc
     3241 catggtgctg cagtcggcgg gcttccaggt tttcgaagcg cgcgacggcg tcgaagcgct
     3301 ggaggccgcg cggaattttc atccggccat catcgtgatg gacatgttca tgcctaacat
     3361 ggacggactg acggcgacgc gcgcgctgcg cgacgaaccg gatctgcgcc gcattcccgt
     3421 gatcgcacag accgcgcaac ccagcgtcat cagcggctcg cgcgagttgt tcgacgccgt
     3481 gctggccaag ccctgttcgc cggacatcct gatccgcacg atctccgagc tgaccacccg
     3541 ctgagaaatg gggtcagacc ccatttcccg gatatggggc ctgaccccat ttcaggcaat
     3601 cgtcagagcg atctcatcgg aatagaagtt ggactcgcgg cacgcgatgc gcagcgtgcc
     3661 ggtcgagcgc cgcagcgcct ttccggggat ggtcatcttc agcgtgccgg cgcgcgcgca
     3721 ggcgagctca cgccaatggt catcgccgtg cggccgatag agcgcgaccg cattcgcgca
     3781 cagcgccgcg cgatatcgca gcttccattc cagcaccgcg tcgttgagcg acgtccacgt
     3841 gagcgactgc gcaccgtccg ccagctcgat cggacccagc acggcgttgg cgacgttgat
     3901 caccgcgccc gtctggcaac gtccttcgag cgacggatag accgtgccgc tcgcgagcag
     3961 gtgctccttg atttcgtgat agccccaggc gggattgatc gccttcacca gcgcggcgca
     4021 gccactcaca tacgctgccc cgatcgcgtt gccgaggagc acgctgtgtc cgctgccgcg
     4081 ccattgcggc acacgcgcaa aaccgggcgc ggcgagatgc gccgcctggc gtgcgcattg
     4141 cgcgagcggc tgaacttgtt cgccgaccga cagcacattg atctggttag gcacgcgatc
     4201 gtcagcggcg agcgccatcg ggctgctcgc atcggatgcc gaacacacca ccagtacgtt
     4261 gcgtgccgcg gcgtactgaa tggcgcgctg cacggccggg gtgcccacct cacccgcggg
     4321 cgagacgttg atgatgcagg cgcccatttc gaccgcgtat tcgatcgcat gcgccaggtc
     4381 gagggcctgc gggccggtgc cattggtgaa acggaccggc aggatgcgcg cgtccggcgc
     4441 cacgccggaa aaccgctcga cttcgctgaa ccgtcccgcc gccacgcccg cggcatgcgt
     4501 gcccgcaatt tcatgactgc ccgcggcagc gaggttgtga gcgagatccc gctggatgtt
     4561 gggaccgaga agcgggtggt cgacctgcac gccttcatcg acgatgccga tgacgacgtc
     4621 ggcgctaccg cgtgtcctgt tccaggcgcg ttcgacaccc agccattcga aggctgcgcc
     4681 gaagcggccc tggttggccg ccggaatcgt catgaatcgt tcccttgcct tgtttgcggt
     4741 gatcagccgc aaaaggtttc tgcgacgtca cgccaagacg tgacaccggg aactcagaat
     4801 ttgacgaaac cgaagtcgag ggcttcctcg cccgacgcct gcttgcgcac ccgaagcgag
     4861 taggggccgg gtcgaagttg tttgctgtcg atcaccacga tcaggctgac cggggagttc
     4921 gggcgctggt cgttcagcgt cgcgacggtg tcgatctttt tgttctgctc gagccggtcg
     4981 agcgcgacgc tgtattgatg ttcgacatcg aacaggccga cgatctccac gcgcaccatg
     5041 ccgggcggca gcgtcggcgc ggtatccgag ccgcgcacca gcgcgaggcg cagcgaggta
     5101 ccgtcacgcg gcgaggagtc ggtgaatgcc gccagtatct ggggggcagc aatccgagaa
     5161 ccgggattcc agagatagaa catcgccacg atggccacca gcacgctcgc ggccgcggcc
     5221 aacgtccagc gcaacgggtg tggagcccgc acgaattccg ccgtcgaacc ccgcgcgatc
     5281 gtgccgatgc cggccttcag gcgctgctcg tactcgacct gccgcgcgaa ttccgggttt
     5341 gcgacgcaat agtcctcgaa ggcctgggcg tcgggttcgc tcagccgtcc ggcgacatat
     5401 tgttcaatga gttcctgatt cataaaccga ggctcaggag gtcgcgtttg gcgaagcccc
     5461 gctcttcaag gatctgccgg aatcgccgcc gcgcacggct caacgcctgg ttgaacgccg
     5521 cctcggacaa gtcgagttcc tgacaaatca gctctttgtc atgatcatcg agatagaagc
     5581 gtttgagcat caggcgatcc cggggttggg gcaattcgcg caagagcgcc cggacagcga
     5641 gggcggcctc gagatccgat gcgatccggt cgacgctttg cgtcggatcg gcgttccgtt
     5701 ccgtcgcttc gctatcaaca tctgttctct gtcgctcagc ctttcgcagt gacgcgatag
     5761 cgagatttct tgctgtttgc gccgcaaatg cagctagttt ctgtgggtca tctatgcctt
     5821 cgccgcgtag gcgctggatg acaaccagca gggtctcctg gacgagatcc tgcaaaaggc
     5881 catggtcgcc gcccgtccgg cgccgcaaaa gcacgcgcag acccggctca aactgacgaa
     5941 tgagctcgct ttcggcggag atatctcctt gccggattcg gcgcgatagc tccccgcctg
     6001 gaacgaaatc catagcctta gatccctgac ccctgaatgc tgacttctga atattcgtta
     6061 ttcagatagt tgcggatagg attctgccac ggcctgccgg cgcaatcagt gaagtaggaa
     6121 aatcctgtgc tcaagacata tcacggttcg tgccactgcg gagccgtgcg cttcgaggcc
     6181 gatatcgatc tgacgcagcc cacgtaccgc tgcaattgct cgatctgctc gcgcaatcgc
     6241 ttctgggcgg cggtggtgaa accgggtgct ttccgtctgc tggaaggcaa atccgcggtg
     6301 cagcagtacc tcttcaactc catgcagaac gagcattttt tctgcaggac ctgtggcgtt
     6361 cgcgcctacg gactcggcaa cagcccggac gtgggcgaga tggtgggagt caacatcggc
     6421 tgcctggaag acacgacgcc cgaagagctg gcggcggcgc cgatccgcta ctgcgacggc
     6481 agatacgaca actggcagaa cgagccggcg atcaccagct atctgtgatc agccagcgtc
     6541 agccgggggc aggcccgcga ctggcaggcg tacgaagaac gtagtgccct tgccgggctc
     6601 ggtgacgaag tcgagttcgc cgccgtggcc cttgaccacc acgttgcgcg acaccgaaag
     6661 cccctggccc gtgccgcggc cgacttcctt ggtggtgaaa aacggatcga agatgcgtgg
     6721 gcgaatgttt tcgggaattc cgccgcccgt gtcggcgatt gaaatgacca cctggtcatt
     6781 ctcggtccgc gtggacacga ctatctttcc gcgcccaccg gttcccttga tcttgtcgcc
     6841 gatggcgtgc gccgcattca ccacgagatt cagcacgacc tggttgatct gtccgccgtg
     6901 acaggtcacg cgcggaattt cgccgagatc ggtttccacg tccgcgacgt cgcgatactc
     6961 actgcgcgcg acgacgagcg tgctggtgat ggcgcgattc agatcgacat cgctcatctc
     7021 acgctggtcg gcatgcgaga agtccttcat cgagcgaacg atttcagtga tgcgtgacag
     7081 gccttcgacg acgcgcgtta gcgccggagg cagttcctcg cgcatgtagt ccacgtcttc
     7141 actcgtggcg gggcgcgtct cggtcgcctt gtgaagctca tcgatgtatg cgagcaactc
     7201 gccgacccca tcgcggatga acgtcacgct gtcgttcagg tactgaatgg gggtgttgat
     7261 ctcgtgcgcg acaccggcgg ccagccggcc gatcgattcg aggcggtcgc tcgacgccat
     7321 gcgctcttcg gcatccttgc gatcggtaat gtctattccg gtgccgacga tgccggtgag
     7381 attaccggcc gcgtcacgga gcgggatctt cgaggtgagg atgatgcggt ccatgccgcg
     7441 cgtcttgtcg aacgcgatct cttcccggtt gatgagcggg atgccggatt ggatcagcgt
     7501 ctgctcgtcg gcgaagaatc gttcggcatg ttcgcgcggg aagaaatcga aatccgtctt
     7561 gccgatcagg tcggaaggct cgacgcccat gcgattcgcg acgagcttgt tagccgcggt
     7621 gaagcggctt tgagcgtctt tcgcgtagat gaaagcgggg atcaggtcga tcatggtccg
     7681 cagcacgttt cgctcgtgcg cgagcgcatc cgccaggttg cgacgttcgg cgctggcgct
     7741 ttggccgtcc tcttcgggct cggtctgcat gtgttgcgga aggctgttca cagccctgta
     7801 attaacggcc ccggggccct gatctgtagt agtaggggaa gaaaggtgga atggctcaca
     7861 gcaggcagtc ctccaaggct atttcaaaga ggctaatccg ccccgcgtga tcgaagttaa
     7921 gccttttcga tcgagagttg atcgaaaacc gcatcgaaac ccgcgccttc tggcgcagcg
     7981 cacatcacgc cggccatcac ctgcacgtcg gcggggaaat agccctgccg gatggtcgtg
     8041 aacttttcgc cgtccttcga gcactgcgcc tcgatggaat ccttcctgcg cacgacgcgc
     8101 caccaggccg cgccggtctg cgacacgtct tccatggtcg accagtcgga aaaatcgtgc
     8161 gtgaacacca cgctcgccca acgcttgttc tcgacgaatt cggagccgca cttcatccag
     8221 tgtttttcat cgagacgcac catgagcccc gcctggtcat agagcgccga ata
//
LOCUS       DATDEU010000692         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1592899, whole genome shotgun sequence.
ACCESSION   DATDEU010000692 DATDEU010000000
VERSION     DATDEU010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592899"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VIV63_16620"
     CDS             <1..301
                     /locus_tag="VIV63_16620"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY6126278.1"
                     /translation="HPIALAVKHEESGVLDFLDAAVTSHDFGAPKEHPQFWTAALARF
                     SYDPARSLFADDNSKMLEAARAAGIRWVYGVRHWDSKGSRREHLDHPAVDAVADL"
     gene            complement(351..1727)
                     /locus_tag="VIV63_16625"
     CDS             complement(351..1727)
                     /locus_tag="VIV63_16625"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6126279.1"
                     /translation="MTDTETHLSELAFASLDLVPSVAQGIRDAGFVRATPVQAMTLPI
                     ALTGKDVAGQAQTGTGKTAAFLVALFQRLLTNPMPGRSASGIRALILAPTRELAVQIF
                     NDAEVLGRHTGLKIGVVYGGVDYEKQRKSLQDGIDVLIGTPGRVIDYFKQHVFDMRHA
                     QAVVLDEADRMFDLGFIADIRFILRRLPKPEARQSMLFSATLSQRVLELAYEHMNNPE
                     LVRIEPDRVTADKVRQMIYFPAMEEKIPLLIGLLRQYDARRTMVFVNTKRVAEKLEAY
                     LIANGFKAQALSGDVPQKKRLRFMKEFHSGELAVLICTDVASRGLHIADVSHVFNYDL
                     PQDAADYVHRIGRPARAGAEGDAISFGCEEYAVALPEIEAYITHKIPVFPVKLELLAK
                     DLKPPVYKEWDPEDGPKPMRRSGGGGGGRGGPRRGGPGGGGGRGGPPRRSGGGGGGSR
                     GGPRRGGR"
     gene            2058..>2179
                     /locus_tag="VIV63_16630"
     CDS             2058..>2179
                     /locus_tag="VIV63_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY6126280.1"
                     /translation="MSNPDIVHTTDATFSQDVLKSDKPVLLDFWAEWCGPCKMIA"
BASE COUNT          405 a          704 c          655 g          415 t
ORIGIN      
        1 gcatcccatc gcgctggccg tgaagcacga ggaaagtggc gtactcgact ttctcgacgc
       61 cgccgtcacg tcgcatgatt tcggcgcgcc gaaggaacac ccgcagttct ggaccgcggc
      121 gctcgcgcga ttctcctacg accccgcacg cagcctgttc gccgacgaca attcgaagat
      181 gctggaagcc gcgcgcgcag cgggaatccg ctgggtctac ggcgtgcgcc actgggacag
      241 caagggctcg cggcgcgagc atctcgacca ccccgccgtc gacgccgtcg ccgatcttta
      301 atggggacac tccccatttg ctagcaaatg gggccggacc tcatcaaggc ctatctgcct
      361 ccgcggcgcg ggcctccgcg actaccacca ccaccgccgc cactgcgacg cggcgggccg
      421 ccacggccac caccaccacc cgggccgccg cgacgcggac ccccgcgccc gccgccaccg
      481 ccaccggagc gtcgcatggg cttcggtccg tcttcgggat cccattcctt gtagaccggc
      541 ggcttcaggt ccttggctaa caactcgagt ttcaccggga acaccgggat cttgtgcgtg
      601 atgtatgcct cgatctcggg cagcgcgacc gcgtattcct cgcaaccgaa gctgatcgcg
      661 tcaccttccg cacccgcgcg cgcgggtcgg ccgatacgat gcacgtagtc cgcggcgtcc
      721 tgcggcaggt cgtagttgaa gacgtggctg acgtcggcga tgtgcaggcc gcgcgacgcg
      781 acgtccgtgc agatcagcac cgcgagctca ccgctgtgaa attccttcat gaagcgaagg
      841 cgcttcttct gcggtacgtc gcccgacagc gcctgggcct tgaagccgtt ggcgatcagg
      901 taggcctcga gcttttccgc gacacgcttg gtgttcacga acaccatcgt tcgccgcgcg
      961 tcgtactgcc ggagcaggcc gatgagcaat ggaatttttt cttccatcgc cgggaagtag
     1021 atcatctgcc ggactttgtc ggcggtgacg cgatcgggct cgatgcgcac cagttccgga
     1081 ttgttcatgt gctcgtacgc gagctcgagc acgcgctgcg acagcgtggc cgagaacaac
     1141 atggattgcc gcgcctcggg cttcggcaac cggcgcagga tgaaacggat gtcggcgatg
     1201 aagccgagat cgaacatgcg atcggcttcg tcgagcacca cggcctgtgc gtggcgcatg
     1261 tcgaacacgt gctgcttgaa gtagtcgatg acgcggccgg gcgtgccgat gagcacgtcg
     1321 atgccatcct gcaggctctt gcgttgcttt tcgtaatcga cacccccata aaccacgcca
     1381 atcttcagac cggtgtgtct tccgagcact tcggcgtcgt tgaagatctg cacggccagt
     1441 tcgcgcgtgg gcgcgaggat gagggcgcgg atgccactcg cactgcgccc cggcatcggg
     1501 ttcgtgagca ggcgctggaa cagtgccacg aggaacgcgg cggttttacc ggtgcctgtc
     1561 tgggcctgac ccgctacatc cttgcccgtg agggcaatgg gcaatgtcat ggcctggacc
     1621 ggggtggcgc ggacgaaacc ggcgtcacgg atgccctggg ctaccgaagg caccagatcg
     1681 agcgaagcga acgcgagttc ggaaaggtga gtctctgtat ctgtcattga attgatttac
     1741 ttatgtattg gggacatcga ttgggcctta agcgcatgtg gcgcttgcaa atggcagtcg
     1801 gaccagttaa aaaatgctcc caagacgcgg gcctgctcgt gaccgagcac tccgaaccgc
     1861 aaatccaaag cttccaaacc aaacactggg tcttttaatc catcaggcaa tttgactgcg
     1921 catcggcagt ctaaccgttt gatcgcgtcc tgagcacggc ttcctgcctg ctgcagcgcc
     1981 agctcttcaa aaaaatccca cccaataaac cactcgagtc ccaatgcatt tctgttttcc
     2041 tatggaggtc cttccacgtg agtaacccgg acatcgtcca cactacggac gcaacgttca
     2101 gccaggacgt gctcaagtcc gacaagcccg ttcttctcga cttctgggcc gagtggtgtg
     2161 ggccctgcaa gatgatcgc
//
LOCUS       DATDEU010000693         4499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1738618, whole genome shotgun sequence.
ACCESSION   DATDEU010000693 DATDEU010000000
VERSION     DATDEU010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738618"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2314)
                     /locus_tag="VIV63_16635"
     CDS             complement(<1..2314)
                     /locus_tag="VIV63_16635"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126281.1"
                     /translation="MAQITAPGTVIRNVGNVAFEADAGIPRTVLSNEVSLAVQPLPSR
                     ASIQLARYEAASQSAFTAGPTQCRAGNTFVALDAPAPQGAGQLDPLSPIPMQDTGVAH
                     AGDPIFLRVVDADRNLDGAVVETVDVRVAARSTGDSEIVRLSETGPGTGVFVGYIATN
                     AAAASSDCSLQVERNTELDATYVDPTDTSDAAQADALVDPFGLIFDSQTGAPIDGARV
                     RLIDNSTGLPATVFGDDGVSRYPSEMVTGQFVTDQSGTQYSLPAGVFRFPLVAPGTYR
                     LEILPPGNYSFPSQRTIADLQTLPGAPYRLQQGSFGQPFVVTLAPAVAVDMPLDPSGE
                     GLVMRKTAGQQIATTGDFVQYTLTLENTSETGTFNAVQVIDRLPAGARFRAGSLRLNG
                     VRIADPVVSADGASFTYLHPAIASGESITLRYVVEYTVAMRGMKDAINTAQAIAPGNV
                     RSNEARALVRMNEELFSQKGFIVGRVFEGACDVTGREETGVQNVRVYLEDGRYGVTDE
                     NGHFHFEGLEPGTHTVQLDKLTLPDYLELAPCADRMGHAGRDYSQFAELSPGTLWRSD
                     FVLRQKSAPRGDVKFEFHSSLIPDPDKDGLAEHEAVIRVNGVSAGNTRALVMLPDGFE
                     YVSGSATVDGVVVADSAVKKTGAGELVVTAADGVVTARLGELQPGSVRTVRFRTRSTA
                     SAGGELPVRAVALFDSPAKSGMRTMPVESKLSRGAAIFGRSQFTFTPRFDALKTELLP
                     SDENALRSLINSWRGARDISIRAVGHADAQP"
     gene            complement(2547..3629)
                     /locus_tag="VIV63_16640"
     CDS             complement(2547..3629)
                     /locus_tag="VIV63_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126282.1"
                     /translation="MLIVHKQMRRVGLAVVAVGAIGLSGQAFADGTPSGDIITNTATV
                     NYTVATVAQTPITASTAFTVDTIVRFNLTGGVQVDVASGQPNGVQMYTLTNTSNATSQ
                     FTLTPTNLAGDDFQMNTPGAGTAGVNVYADTNGNGVYNAGTDVQVTANVSLANAGSAV
                     YFLVGDTPLTATNGQIAQVQMAVQAINPATSAAWVNDTNPDTQGGVVGYVPQIVVGNN
                     TATRPGSFRVQTATLAITKSSSVISDPINNTGAGRKAIPGAIMQYQILVANTGATAAT
                     LQNVSDTLPPPASIVFRQGDFTGATDIEIQVGANPPTYCIAQSGADVPADGCFRTGNT
                     LTIQAPAITSVAPGSQVAVRFRMTIQ"
     gene            complement(3661..4155)
                     /locus_tag="VIV63_16645"
     CDS             complement(3661..4155)
                     /locus_tag="VIV63_16645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126283.1"
                     /translation="MFKGNRKVLGVFALMACALGSQAFAQSQGCIVLKSTAEIEKEVV
                     NDKGEKSKVLVAAGKVIPGTEVVWTITANNVCKQPSDKVTINNPVPEHMKLVPNSAFG
                     PGSDVSYSLDGQKFASAGQLTVQENGAARPARADEYKHIRWEFKNSLQPGASAFARFR
                     AVLN"
     gene            complement(4187..>4499)
                     /locus_tag="VIV63_16650"
     CDS             complement(4187..>4499)
                     /locus_tag="VIV63_16650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126284.1"
                     /translation="KSQTVVDQFGGTRPLPTARINYTITVQAVGSGSAANTVFSDNIP
                     ANTTYVPGTLRLNSTALSDAAADDAGEFISTPSARMRVTLGTLTQASGTQTIQFAVTI
                     N"
BASE COUNT          756 a         1442 c         1490 g          811 t
ORIGIN      
        1 tcggctgcgc atcggcatgg ccgaccgcgc ggatgctgat gtcgcgcgcg ccgcgccagg
       61 aattgatgag cgagcgcagc gcattctcgt cggacggcag cagctcggtc ttgagtgcgt
      121 cgaagcgcgg cgtgaacgtg aactgcgagc ggccgaagat cgccgcaccg cgcgacagct
      181 tcgactcgac cggcatcgtg cgcatgccgg atttcgccgg cgagtcgaac agcgcgacgg
      241 cgcgtaccgg cagttcgcca cccgcggagg cggtcgagcg cgtgcggaaa cgcaccgtgc
      301 ggacgctgcc gggctgcaac tcgccgagcc gcgcggtgac gacaccatcc gcggcggtga
      361 cgacgagctc gccggcgccg gttttcttca cggcggaatc ggcgaccacg acgccgtcaa
      421 cggtcgcgct tccggagacg tattcgaaac cgtccggcag catgacgagc gcacgcgtgt
      481 tgccggccga cacaccgttc acgcggatca cggcttcgtg ttccgcgaga ccatccttgt
      541 ccggatccgg gatcagcgac gaatggaact cgaatttcac gtcgcccctg ggcgcggact
      601 tctggcgcaa cacgaaatcg ctgcgccaca acgtgccagg actcagctcc gcgaactgcg
      661 agtagtcgcg tcccgcgtga cccatgcgat cggcgcaggg cgcgagctcg aggtaatcgg
      721 gaagagtcag cttgtcgagc tgcacggtgt gtgtgccggg ctcgaggcct tcgaagtgga
      781 aatggccgtt ctcgtcggtg acgccgtaac ggccgtcttc caggtagacg cggacgttct
      841 gcacgcccgt ctcttcacgt cctgtgacgt cacaggcgcc ttcgaacacg cgaccgacga
      901 tgaagccctt ctgcgagaac agttcttcgt tcatgcgcac cagcgcgcgc gcttcgttcg
      961 agcgcacgtt gccgggcgcg atcgcctgcg cggtgttgat ggcgtccttc atgccgcgca
     1021 tcgcgaccgt gtattcgacg acatagcgca acgtgatcga ttcgccggag gcgatggccg
     1081 gatgaaggta agtgaagctg gcgccgtcgg cgctgaccac cgggtcggcg atgcgcacgc
     1141 cgttgagacg caacgagccc gcgcggaacc gcgcgccggc cggcagccgg tcgatcacct
     1201 gcaccgcgtt gaacgtgccc gtctcgctcg tattctcgag cgtgagcgtg tactgcacga
     1261 agtcgcccgt ggtcgcaatc tgctggcccg cggtcttgcg catcacgagt ccctcgcccg
     1321 acggatcgag cggcatgtcg accgccaccg ccggcgcgag cgtgaccacg aacggctgac
     1381 cgaaggaacc ctgctgcaac cgatacggcg cgccgggtaa cgtctgcaga tcggcgatcg
     1441 tgcgttgcga aggaaaactg tagttgccgg gcggcaggat ctcgaggcga taggtgcccg
     1501 gcgccaccag cggaaaacgg aacacacccg ccggcaggct gtactgcgtg ccactctggt
     1561 cggtcacgaa ctggcccgtg accatctcgg agggatagcg actgacgccg tcatcaccga
     1621 agaccgttgc cggaaggcct gtcgaattgt cgatgaggcg aacgcgcgcg ccgtcgatcg
     1681 gcgcacccgt ctgggaatcg aagatgagac cgaacggatc gacgagtgca tcggcctgcg
     1741 cggcgtcgct cgtatccgtg gggtccacgt aggtggcgtc gagctcggtg ttgcgttcga
     1801 cctgcaacga acagtcactc gacgccgcag ccgcattggt tgcgatgtag ccgacgaaca
     1861 ccccggtgcc cgggccggtt tccgaaaggc ggacgatttc cgaatcaccc gtgctgcgcg
     1921 ccgccacgcg cacgtccacg gtctcgacga ccgcaccgtc gaggttgcgg tcggcatcca
     1981 ccacgcgaag gaagatcgga tcgccggcgt gcgccacgcc ggtgtcctgc atgggaatcg
     2041 gcgacagcgg atcgagctgg cccgcaccct gcggcgcggg tgcatcgagc gcgacgaacg
     2101 tgttgccggc gcggcactgg gtcgggcccg ccgtgaacgc gctctggctc gccgcttcgt
     2161 agcgggcgag ctggatcgac gcgcgcgacg gcagcggctg aaccgccagg ctgacctcgt
     2221 tcgacagcac ggtgcgggga atgcccgcat cggcttcgaa tgcaacgttg cccacgttgc
     2281 gaatcaccgt tccgggcgcc gtgatctgcg ccacggaaac atttgcgaca gtgagacacg
     2341 ccgcaaataa caacgaagtc cggagcagcg cgtttacgcg gctcctaaac gcgccgaagg
     2401 cggggtagct agtcgctacc ccgccctctg cgagagagta cccgttgttg cgggagtcgg
     2461 ccttggggcg ccctttgggg ctccaggacc gccgacccgc ggagtcaatc atcatgacgc
     2521 gttgttacct tccgtctgcc aatcgactac tggatcgtca tgcggaagcg cacggcgacc
     2581 tgcgagcccg gagcaaccga cgtgatcgcc ggagcctgga tcgtcagcgt attacccgtg
     2641 cggaagcagc catcggcggg aacgtccgca ccagactgcg cgatgcagta ggtgggcgga
     2701 ttggctccga cctggatctc gatatcggtc gcgcccgtga agtcaccctg gcggaacacg
     2761 atgctcgccg gcggcgggag cgtatcgctg acgttctgca acgtcgccgc ggtcgcaccc
     2821 gtgttcgcca cgaggatctg gtactgcatg atcgcgcccg ggatcgcctt gcggccggca
     2881 cccgtgttgt tgatcggatc actgatcacc gagctcgact tggtgatggc cagcgtggcg
     2941 gtctgaacgc ggaacgatcc aggacgggtc gccgtgttgt tgcccacgac gatctgcggg
     3001 acatagccga cgacgccacc ctgcgtatca ggattggtgt cgttgaccca cgcggcactg
     3061 gtcgccgggt tgatggcctg cactgccatc tggacctgcg cgatctggcc gttggtcgcc
     3121 gtcagcggcg tgtcgccgac gaggaagtac accgccgacc cggcatttgc cagcgagacg
     3181 ttcgccgtca cctggacgtc ggtgcccgcg ttgtagacgc cgttgccgtt ggtatcggca
     3241 tagacgttga cgcccgccgt tcctgcgccc ggcgtattca tctggaaatc gtcgccagcc
     3301 aggttggtcg gggtgagcgt gaactgcgac gtcgcgttgc tggtgttcgt caacgtgtac
     3361 atctgcacac cgttgggttg accagacgcc acgtccacct gcacaccacc cgtcaggttg
     3421 aaccggacga tggtgtcgac tgtgaacgca gtgcttgccg tgatcggggt ctgggcgacc
     3481 gtcgccacgg tgtagttgac ggttgcggtg ttggtgatga tgtcaccgga aggcgtgccg
     3541 tcggcaaacg cttgtcccga taaaccgatc gccccaaccg caaccacggc gagaccgact
     3601 cgccgcattt gcttgtggac aattaacatg ggtactcctc tctactctct aagaaaaaaa
     3661 ctaattcaga accgcacgga agcgcgcgaa agcgctcgcg ccgggctgca gggagttctt
     3721 gaactcccag cggatatgct tgtattcatc ggcacgcgcg gggcgcgcgg cgccgttctc
     3781 ctgcaccgtg agctggccgg ccgatgcgaa cttctggccg tcgagcgaat agctgacgtc
     3841 gctgcccggg ccgaaagccg agttcggcac cagcttcatg tgttcgggca cggggttgtt
     3901 gatcgtcacc ttgtccgacg gctgcttgca gacattgtta gccgttatcg tccagacgac
     3961 ttccgttccc gggatgacct ttccggccgc caccaggacc ttggatttct cgcccttgtc
     4021 gttgacgact tccttttcga tctcggctgt cgacttgagg acgatgcatc cctgcgactg
     4081 cgcgaatgcc tgcgagccaa gcgcgcaggc catgagggcg aaaacgccca gaactttccg
     4141 attccccttg aacatgtgtg tctccttcac agtcgtactt cccggatcaa ttgatggtta
     4201 cggcgaactg gatcgtctgg gtcccgctcg cctgggtgag agtgccgagc gtcacgcgca
     4261 tgcgtgcgct cggtgtgctg atgaactcgc ccgcgtcgtc ggcggccgcg tccgagagcg
     4321 ccgtgctgtt gaggcgcaac gtcccgggca cgtacgtcgt gttcgcggga atgttgtcgc
     4381 tgaagacggt gttcgcggcg ctgccgcttc ccactgcctg gacggtgatc gtgtagttga
     4441 tgcgcgccgt cggcagcggg cgcgtgccgc cgaactggtc gacgacggtc tgcgacttc
//
LOCUS       DATDEU010000694         3437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_573890, whole genome shotgun sequence.
ACCESSION   DATDEU010000694 DATDEU010000000
VERSION     DATDEU010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573890"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VIV63_16655"
     CDS             <1..848
                     /locus_tag="VIV63_16655"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126285.1"
                     /translation="NDVVRRCLYLFAALPRADGLVAACVFEKPVARYGGLHIVDYAAL
                     FNVTLFDYVDATGDVATGRDLWPAALRQLEILGRDVNAEGLYVDPKNMWIFIDWSDKL
                     DRNAAIQGVMIYAWQKTLALARRIGKETDVADYASRIERMTAAARKHYWDASRRVFVS
                     GPNRQISWGSQAWMAIAGVSSRRESADAMRTAVADPNAIRPVTPYLYHYVVEGMLAGG
                     LRKEARALMESYWGGMIAAGADTFWEIYDPAQPLRSPYGDIHINSYCHAWSCTPSYLL
                     RSGLL"
     gene            845..1951
                     /locus_tag="VIV63_16660"
     CDS             845..1951
                     /locus_tag="VIV63_16660"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6126286.1"
                     /translation="MNVPVSSIQTTLYLIAVALCIFSTGLTTRRAGRGMPLRFFTWYL
                     LIQSAAFVCELLIAHPSTPLKALWLGLLMSGSLLVAPCLWLAFQESIGGSRPGLSTLT
                     RGHWLAVVAGVLLTVPLMLSAHLGNTFSNPSQPSSWLQSRIIHTTMLMCIGIFVVQVP
                     WYLLRCRQILIERLQGRRNHWAQLPLAIVFTSWALAILRTIDTAFMKWPPMFSAIVAV
                     ISVSITAGALYLLLREFSAPESGAADRYARSPLGDAVRTRIRRKLETKLGEEAIYKRS
                     DLSLRTLSEAINESPHYVSQVISQDLNSSFYELVNRYRIEQAKRLLRQSPEESVLSIA
                     MNVGFNSKSAFHAAFRRCTGARPSDFRESSTRQG"
     gene            complement(1942..2718)
                     /locus_tag="VIV63_16665"
     CDS             complement(1942..2718)
                     /locus_tag="VIV63_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEY6126287.1"
                     /translation="MISETGGIPGGFRLVWSDEFDVAGLPDSTRWNYDIDRNSAGWYN
                     NELQYYGNARLENSRVESGMLLITARREDLSTAGVPDWSGQRYSSARLVTRGKASWTY
                     GFIEVRAKLPCGLGSWPAIWMLSTPPEARWPDDGEIDIMEHVGFDPGVVHATAHTGAF
                     NHTRGNHRTATTTIPDACNNFHRYQMNWTASRITVGVDDRYYYQYSNDNSGNAEWPFD
                     SPQYLILNIAVGGDWGGQMGVNDAIFPITMEIDYVRVYQP"
     gene            3019..>3437
                     /locus_tag="VIV63_16670"
     CDS             3019..>3437
                     /locus_tag="VIV63_16670"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6126288.1"
                     /translation="MNKSNVLRRTPVAQAISLILGSTLLAPAIAQEAGSSPEDVGEIV
                     VTGIRASLESSMNLKRAGQGVIDGIVAEDIGKFPDTNLAESLQRISGVSINRTASGEG
                     QQVTVRGVGPDFNLVLLNGRQMPASNLGPGGGGTTNSR"
BASE COUNT          615 a         1055 c         1073 g          694 t
ORIGIN      
        1 ccaacgacgt ggtgcggcgc tgcctgtacc tgttcgcggc gctgccgcgc gccgacggac
       61 tcgtggccgc ctgcgtgttc gagaagccgg tcgcgcgcta cggcggcctg cacatcgtcg
      121 actacgccgc gctgttcaac gtgactctgt tcgactatgt ggatgcgacc ggcgatgtcg
      181 ccaccggccg cgacctgtgg cccgcggcat tgcgacagct tgaaattctc ggtcgcgacg
      241 tgaatgccga agggctgtac gtcgatccga agaacatgtg gatcttcatc gactggtcgg
      301 acaagctcga tcgcaacgcg gcgatccagg gcgtgatgat ctatgcctgg caaaaaaccc
      361 tcgcattggc tcgccgcatt ggtaaggaaa ccgatgtcgc cgactatgcg agccgcatcg
      421 aacggatgac cgccgccgcc cgcaagcact attgggatgc gtcgcgccgg gtgttcgtca
      481 gcggcccgaa ccggcagatc tcctggggct cacaggcgtg gatggcgatt gccggcgtct
      541 cgtcgcgacg cgagagcgcg gatgccatgc ggacggccgt ggcggatccc aacgccatca
      601 ggccggtcac cccttatctc tatcactacg tcgtcgaagg catgctggcc ggtgggcttc
      661 gcaaggaagc gcgcgcgctg atggagtcct actggggcgg aatgatcgcc gcgggcgcag
      721 acacgttctg ggaaatctac gatccggcgc agccgcttcg ttctccctac ggcgacatcc
      781 atatcaacag ctactgccac gcgtggagct gcacgccttc ctacctactg cgcagcgggt
      841 tgttgtgaac gttccagtca gctccatcca gaccacgctt tatctcatcg ccgtggcgct
      901 gtgcattttc tccaccgggc tcaccacgcg cagggcaggc cgcggcatgc cgctgcgctt
      961 tttcacctgg tacctgctca ttcaatcggc tgcgttcgtc tgcgagctct tgatcgcgca
     1021 cccgtcgaca cccctcaagg ctctctggtt ggggctcttg atgtcgggtt cgttgctggt
     1081 ggcgccgtgt ttgtggctgg cattccagga gagcatcggt ggctcgcggc ctggactctc
     1141 cacgctcacg cgtggccact ggctggctgt cgttgccggt gtactgttga ccgtgccgct
     1201 catgttgtcg gcgcatctcg gcaacacgtt ttccaatccc tcgcaaccgt caagctggct
     1261 ccagtcgcgc atcattcata ccaccatgtt gatgtgcatc ggcatcttcg tcgtccaggt
     1321 gccgtggtac ctgctgcgct gccgacagat tctcatcgag cggctgcagg gccggcgcaa
     1381 tcattgggcc caactgccgt tggccatcgt gttcacctcg tgggcgctcg ccatcctgcg
     1441 cacgatcgac accgcgttca tgaaatggcc gccgatgttt tcggccatcg tggcggtgat
     1501 cagcgtcagc atcaccgcgg gcgcgctgta tctgctgctg cgcgagttca gcgcaccgga
     1561 gtccggcgcc gccgatcgat acgcccgctc gccgctgggc gatgcggttc gcacgcgcat
     1621 ccgccgcaag ctcgagacga agctgggcga agaagccata tataagagaa gcgacctctc
     1681 gctgcgcacg ctcagcgagg caatcaacga gagtcctcac tatgtctcgc aggtgatcag
     1741 ccaggatctg aactcgagct tctacgagct cgtgaaccgt tatcggatcg agcaggcaaa
     1801 gcgtctgctg cggcaatcgc cggaggagtc cgtgctgtcc attgcgatga atgtcggctt
     1861 caattccaag tcggcgtttc acgccgcgtt ccggcgatgc acgggtgccc ggcccagcga
     1921 tttccgcgaa tcatcgacgc gtcagggctg atagacgcgc acgtagtcga tttccatcgt
     1981 gatcgggaag atggcgtcgt tcacgcccat ctggccgccc cagtcaccac cgacggcaat
     2041 attgaggatc aggtactgcg ggctgtcgaa aggccattcc gcgttgccgg agttgtcgtt
     2101 cgaatactgg tagtagtagc gatcgtcgac gccaacggtg atgcgactgg cggtccagtt
     2161 catctggtac cgatggaagt tgttgcaggc atccgggatc gtggtggtgg cggtgcgatg
     2221 gttgccgcgc gtgtggttga acgcacccgt gtgtgcggtg gcatgcacga caccggggtc
     2281 gaaacccacg tgttccatga tgtcgatctc gccatcgtcg ggccagcgcg cttccggagg
     2341 cgtcgacaac atccagatcg ccggccacga accgagaccg cagggtagtt tggcgcgcac
     2401 ctcgatgaag ccgtaggtcc acgatgcctt gccgcgagtg acgagccttg ccgaggagta
     2461 acgctggccg ctccagtccg gcaccccggc ggtagatagg tcctcgcgcc gggcggtgat
     2521 gagcagcatg ccgctctcca cgcgtgaatt ctccagtcgc gcattgccgt agtactgcag
     2581 ctcgttgttg taccagcccg ccgaattgcg atcgatgtcg tagttccagc gcgtcgagtc
     2641 gggcaaaccg gcgacgtcga actcgtcgga ccacacgaga cgaaaaccac cgggaattcc
     2701 gccggtttcg gagatcacgg gtgcgccagg tcccgtcggc tgcggaggcg ttggtgaggt
     2761 gacaggggga ctgccagggc cttcacctcc gcatgccgac gagaaaagtg ccgcggttac
     2821 cgctatcacg gctaacccgc gggttgaatt cacgcgacta atgaaagtcg aaatctgaga
     2881 catcgaaaac gacatgcagg acatgtgttt gtcatctctt gtttgcttgc cggagcgtgg
     2941 gctgcatgct agcgtaaaaa ttgtaagcgc ttacatttat ttgctttgac gcttacacaa
     3001 tttttaccgt gggggcatat gaacaagtcg aacgtactca ggcgcacgcc agttgcgcag
     3061 gcaatctcgt tgatcctcgg atcaacactt ctggcgccgg caattgcgca ggaggcgggc
     3121 agctcgccgg aagatgtcgg cgaaatcgtg gttactggca ttcgagcgtc actcgaatcg
     3181 tcgatgaatc tcaagcgcgc cggtcagggc gtcatcgacg gaatcgtcgc ggaagacatc
     3241 ggcaaatttc cggataccaa tctcgcggag tcgctgcaac gcatcagtgg cgtatccatc
     3301 aaccgcacgg cgagtggtga gggccagcag gtcacggtcc gcggcgtagg cccggacttc
     3361 aacctcgtgc tgctcaatgg ccgccagatg ccggcatcga atctgggccc gggcggcggc
     3421 ggcacgacga attcacg
//
LOCUS       DATDEU010000695         4010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_864523, whole genome shotgun sequence.
ACCESSION   DATDEU010000695 DATDEU010000000
VERSION     DATDEU010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864523"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /gene="dtd"
                     /locus_tag="VIV63_16675"
     CDS             <1..518
                     /gene="dtd"
                     /locus_tag="VIV63_16675"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY6126289.1"
                     /translation="KTGRDIKDLGAQLPPGTIPRVITLIQRVSEAAVTVDDRIVGRIG
                     GGILALIGFERGDGPAQIDKLLTRVLTYRIFPDEAGRMNLSLADTGGGLLLVPQFTLA
                     ADTQKGTRPGFSTAAAPELARTLFAECVALARARHADVSEGEFGAHMRVSLINDGPVT
                     FWLRTAPDGKE"
     gene            646..879
                     /gene="tatA"
                     /locus_tag="VIV63_16680"
     CDS             646..879
                     /gene="tatA"
                     /locus_tag="VIV63_16680"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY6126290.1"
                     /translation="MGPIGFKELLVILVVVLLIFGAKKLKTIGSDLGSAVRGFKKSMN
                     DGDAEESVKQLKADAEFSDSAKSQTAEKSETKA"
     gene            925..1368
                     /gene="tatB"
                     /locus_tag="VIV63_16685"
     CDS             925..1368
                     /gene="tatB"
                     /locus_tag="VIV63_16685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY6126291.1"
                     /translation="MFEVGFTELLLIFALALVVLGPERLPKLAQQVGRWVGRARAMAR
                     QFRDQLEEEAHNLETKVNIDPGIDTSLDPKPAAQTPPTPATPAATNLPVPIVEPEEEF
                     YPPDHHMHPSQHVDTPAQPSPDPQADSQTELDLTTPRDDDSRRVP"
     gene            1371..2162
                     /gene="tatC"
                     /locus_tag="VIV63_16690"
     CDS             1371..2162
                     /gene="tatC"
                     /locus_tag="VIV63_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY6126292.1"
                     /translation="MSERDEKKGEDLAEGTLMSHLLELRNRLLKAMLGILVAFVPCAI
                     YANNLFTMIAQPLIDKLPKGASLIATNVVSPFMTPFKVAFYVALFVAMPVVLYQIWAF
                     VAPGLYRREKRFAVPLLVSSVLLFYAGAVFAYFAVFPVMFTFLVGTAPTGVSYMPDMT
                     SYLDFVITLFFAFGIAFEMPVAVVLLVLTGLVNVETLGKNRGYVIIGIFVVAAFLTPP
                     DAISQTIMAVPMYLLYESGLAMARVMQKMRREAAEAAEKNEAEAN"
     gene            2276..3805
                     /locus_tag="VIV63_16695"
     CDS             2276..3805
                     /locus_tag="VIV63_16695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEY6126293.1"
                     /translation="MSEPITTAAGSPDYSRSFFGHPRGLATLFMTEFFERFTYYGLRA
                     MLVLFLAASATGANPGFGVDGATAGAVYALFTGAAYLCCIPGGWIADRLLGQRSAVFT
                     GGVFIAIGNFILAIPASPAIFYIGLAVVVLGIGLLKPNVSSVVGALYEGQAGARRDAG
                     FSIFYMGINLGAAAGPFIAGGLGEGWNWRLGFLTCGFAMLIGLAQFKMTEKYLGSAGY
                     APQIPPEQRKRSWTLVAAVTVAVLIGASFLFMRENVPSETALAQGLFIAQVAIAVGFF
                     GYMLFFGGLTTVEKKRVGVIVIFFLSAALFWGGFEQQATTFNTFAVDYTDRSWLGSMF
                     PESMHPASWYQSINPVCIIVFAPFFAWIWVALGARNLDPSAPFKMGMGLVLLGVGFLI
                     MMWAAQLVVSSGGKVGPTWLLLAYMIHTFGELCLSPVGLSNVTKLAPAKFVSSLMGTW
                     FLGSAIGNTVAGLVGGHSASLKADELPHQFLIMTLIGGGAGVFIMLLARPMRSWIGDR
                     K"
     gene            3802..>4010
                     /locus_tag="VIV63_16700"
     CDS             3802..>4010
                     /locus_tag="VIV63_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126294.1"
                     /translation="MSTSNYLRGASAIALTVSGACLLASCNGPPGPKPPPKIEAPVFV
                     EQLNKDLVDLNREANAAGWTQATDIT"
BASE COUNT          690 a         1314 c         1233 g          773 t
ORIGIN      
        1 cgaaaacagg ccgcgatatt aaggacttgg gcgcgcaatt gccaccgggc acaatccctc
       61 gggtgataac actcattcaa agggtcagcg aagcagcggt gacggtcgac gaccggatcg
      121 tgggccggat cggcggcggc atccttgccc tcatcgggtt cgagcgaggc gatgggcccg
      181 cgcagatcga caagctgctc acgcgtgtac tcacctatcg aatcttcccc gacgaggccg
      241 ggcgcatgaa tctctcgctc gcggacacgg gcggcggtct cttgctggtt ccgcagttca
      301 cgctggccgc cgatacccaa aagggcacgc gaccgggatt cagcaccgcg gcagcgcccg
      361 aactcgctcg caccctgttc gcggaatgcg tggcgctggc gcgcgcccgg catgcggatg
      421 tgtcggaagg ggaattcggc gcccacatgc gggtctcact gatcaacgac gggccagtca
      481 ccttttggct caggacggcc ccggatggga aagagtgaca aaggctcgtg atgggggtct
      541 cgccccggcc gaactttctt aggtttggca cctctgttga cttatcattc cggcacttat
      601 ctatgacgta cgtcgggggc gacccgaccc ccacaggagc ggctcatggg ccccattggt
      661 ttcaaagaac ttctagtcat cctggtcgtg gttttgctca tcttcggcgc gaagaagctc
      721 aagacgatcg gttctgacct tggctccgcg gtacgcggct tcaagaagtc gatgaatgac
      781 ggcgatgccg aagagtcggt caagcagctc aaggccgatg ccgagttttc cgattccgca
      841 aaatcccaga ccgcggaaaa gagcgagacc aaggcttaag cctctggcgg ccgccatcgc
      901 atttcgcgcg gcggagcatc ccccatgttc gaggttggat tcactgaact gctgctgatc
      961 ttcgcgctcg cactcgtcgt gctgggcccg gagcggctgc cgaagctcgc ccagcaggtg
     1021 ggccggtggg tcggacgcgc acgcgccatg gcgcgccagt tccgtgacca gctcgaggaa
     1081 gaggctcaca acctcgagac caaggtgaac atcgatcccg gcatcgacac ctcgctcgat
     1141 ccgaaacccg cggcgcaaac cccgccgacg ccggcaacac cggccgccac gaacctgccg
     1201 gtgccgatcg tcgagccgga agaagagttc tatccgcccg atcaccacat gcatccgtcg
     1261 cagcacgtgg acacaccggc gcaaccctcg cccgaccccc aggccgattc gcagaccgag
     1321 ctcgacctaa ctaccccgcg cgacgacgat tcgcgccgcg ttccctgagc gtgagcgagc
     1381 gcgacgaaaa gaagggcgaa gacctggccg aaggtacgct gatgtcgcac ctgctcgagc
     1441 tgcgcaaccg gctcctcaag gccatgctcg gcatcctcgt ggccttcgtg ccgtgcgcga
     1501 tctacgccaa caacctgttc acgatgatcg cgcagccgct catcgacaaa ctacccaagg
     1561 gcgcgtcgct gatcgcgacc aacgtggtct cgccgttcat gacgcctttc aaggtggcgt
     1621 tctacgtggc gttgttcgtc gcgatgcccg tcgtgctgta ccagatctgg gccttcgtcg
     1681 cgcccggcct gtaccggcgc gaaaaacgct tcgccgtgcc gctgctcgtt tcctcggtac
     1741 tgctgttcta cgccggcgcc gtgttcgcct acttcgcggt gttcccggtg atgttcacgt
     1801 tcctcgtcgg caccgcgccc acgggcgtct cgtacatgcc ggatatgacc agttatctcg
     1861 acttcgtgat cacgctgttc ttcgcgttcg gcattgcgtt cgagatgccg gtggccgtcg
     1921 tgctgctggt gctcacgggc ctcgtgaacg tggaaacgct gggcaagaat cgcggctacg
     1981 tgatcatcgg catcttcgtg gtggccgcgt tcctgacccc cccggacgcc atttcccaga
     2041 ccatcatggc cgtgcccatg tacctgctct atgaaagcgg gctggcgatg gcacgcgtaa
     2101 tgcagaagat gcgccgcgaa gcagcggaag cggccgagaa gaacgaagcc gaagcaaatt
     2161 agcgcgggga cgaaccgagc gcgcaccgct gcgccttgct ggccccggca cgcttgagtc
     2221 agactcggcg gcatcaaggc gcaacagcgc ctacaaaaaa ttcggggcaa aaataatgag
     2281 cgaaccgatc acgacggccg cagggtcgcc ggactacagc cgcagctttt ttggtcatcc
     2341 gcgtggactc gccacgttgt tcatgaccga gttcttcgag cgattcacct actacggact
     2401 gcgggccatg ctcgtgttgt tcctcgcggc ctcggccacg ggcgccaacc cggggttcgg
     2461 tgtcgatggc gcgaccgccg gcgcggtgta cgcgttgttc acgggcgccg cttacctgtg
     2521 ctgcatcccc ggaggctgga tcgccgaccg gttgctcggt cagcgcagtg cggtattcac
     2581 cggcggcgtg ttcatcgcca tcggcaattt catcctggcc attcccgcgt ccccggccat
     2641 cttttatatc ggcctcgcgg tggtcgttct cggcatcggc ctgctgaagc cgaatgtctc
     2701 gagcgtggtc ggagcactct atgaaggtca ggcgggagcg cggcgcgatg ccggcttctc
     2761 catcttctat atgggcatca acctgggcgc ggcggctggt ccgttcattg cgggcggcct
     2821 gggcgaaggc tggaactggc gcttgggttt cctcacctgc ggcttcgcga tgctcatcgg
     2881 tctcgctcag ttcaagatga cggagaaata tctgggcagc gcggggtatg cgccgcagat
     2941 acctcccgaa cagcgcaaac gctcctggac gctggtggcc gcggttaccg tcgcggtact
     3001 catcggcgcc agcttcctgt tcatgcgcga gaacgtgccc tcggaaaccg cgctggcgca
     3061 gggattgttc atcgcgcagg tcgccatcgc cgtcggcttc ttcggctaca tgctgttttt
     3121 cggtggacta accaccgtcg agaaaaagcg tgtcggcgtc atcgtcatct tcttcctgag
     3181 cgcggcactg ttctggggcg gcttcgagca gcaggccacc acgttcaata cgttcgcggt
     3241 cgattacacc gatcgctcct ggctcggcag catgttcccg gagagcatgc atcccgcgtc
     3301 ctggtaccag tcgatcaacc cggtctgcat catcgtcttc gcgccgttct tcgcgtggat
     3361 ctgggtcgcg ctgggcgcac gcaatctcga tccttccgcg ccgttcaaga tgggcatggg
     3421 cctggtgttg ttaggcgtcg gcttcctcat catgatgtgg gcggcgcagc tcgtggtctc
     3481 gagcggcggc aaggtcggtc ccacctggct cttgctggcc tacatgatcc atacgttcgg
     3541 cgagttgtgc ctgtcaccgg tcggtctgtc gaacgtgacc aaactcgcgc ccgcgaaatt
     3601 cgtgagcagc ctcatgggca cctggttcct cggcagcgcc atcggcaaca cggtggccgg
     3661 actcgtcggc ggacattccg cgtcgctcaa ggccgacgag ttgccgcacc agttcctcat
     3721 catgacgttg atcggcggcg gcgccggcgt attcatcatg ctgctggcgc gcccgatgcg
     3781 cagctggatc ggagaccgga aatgagcacg tccaactacc tgcgcggcgc gtccgccatt
     3841 gcgctcaccg tctccggcgc ctgcctgctc gcttcctgca acggcccgcc cggacccaag
     3901 ccgccgccga aaatcgaagc gccggtgttc gtcgaacaac tcaacaagga tctggtcgat
     3961 ctcaaccgcg aggcgaacgc cgccggctgg acccaggcca ccgacatcac
//
LOCUS       DATDEU010000696         2480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1157248, whole genome shotgun sequence.
ACCESSION   DATDEU010000696 DATDEU010000000
VERSION     DATDEU010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157248"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /gene="pilW"
                     /locus_tag="VIV63_16705"
     CDS             <1..622
                     /gene="pilW"
                     /locus_tag="VIV63_16705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY6126295.1"
                     /translation="YLKQGNYPVAKEKLERSLKQNPNDPDVHTALATLYDRVNEPKLA
                     DKHFREALRLAPANPDISNNYAVYLCKNGRIDEGVERFSAVATNKYYRTPEVALSNAG
                     VCLRGARKFDEAQQMFTRAIKVRPNYSEATVQLATLHVERGQVAEARKVVDTYLGAFR
                     PDPDVLYAAVNVARAAKDKMGEEKFSRTLRLEFPDSAQARALKRGS"
     gene            631..1518
                     /locus_tag="VIV63_16710"
     CDS             631..1518
                     /locus_tag="VIV63_16710"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY6126296.1"
                     /translation="MDETAATATPPAPRSIGERLRAGRERSGLSIAAAAEKLHLDAKV
                     IEALEADRFAELGASVYVRGHLRRYGDFVGEAGPELVGLYAARDARPPPPDLTQIPQA
                     ERRADPRRLVTPFVGLSCAAVLLVAIWWVLAGSRSGSSEVATALPTPVVQPDIEVAAN
                     TAPGVATPVSMASSTPVTPPATAPAGSAVTTPAAAAQPKREEVAPARETRLKLDLTND
                     SWVEVYDSRGERLFFDVASAGSVQSVSGRGPLRVVLGHAAGVSVEVDGQSREIPSAAI
                     DGESASFVVNRSGSLSRAR"
     gene            1531..>2480
                     /gene="hisS"
                     /locus_tag="VIV63_16715"
     CDS             1531..>2480
                     /gene="hisS"
                     /locus_tag="VIV63_16715"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY6126297.1"
                     /translation="MSRTIPPVTGMKDVLAADIALWQHVERCWRTVADAYGYQEMRVP
                     VVEHTELFKRAIGEYTDVVEKEMYTFTDQGGDSLTLRPEATAGIVRATISNGLLRGAR
                     LKLWCQGPMFRHEKPQKGRFRQFNQIDLEAIGFAGPDVDIEIIALTARLWKLLGVTRV
                     KLQINSLGTPESRKAYRTLLQDYFRQHRDALDEDGKRRLEGNPLRILDSKHPPTQAIV
                     GNAPVLTEHLDAESAEHFTQLRAGLDALGIPYTVNPRLVRGLDYYSRTVFEWITDALG
                     SQDAVCSGGRYDGLIAQMGGDATPAIGWAMGVERVVELVRL"
BASE COUNT          432 a          871 c          802 g          375 t
ORIGIN      
        1 ctatctcaag cagggcaact acccggtcgc caaggaaaag ctcgagcgct cgctcaagca
       61 gaaccccaac gacccggacg ttcacaccgc gctcgcgacc ctgtacgacc gcgtcaacga
      121 accgaagctc gccgacaagc atttccgcga agcgctgcga ctggcgcccg ccaaccccga
      181 catctccaac aactacgccg tctacctctg caagaacggc cgcatcgacg agggcgtcga
      241 gcgtttttcg gcggtcgcga ccaacaagta ctaccgcaca cccgaggtgg cgctctccaa
      301 cgccggcgtc tgcctgcgcg gcgccaggaa attcgacgag gcgcagcaga tgttcacgcg
      361 cgccatcaag gtccggccta actacagcga agccaccgtg cagctcgcga cgctgcacgt
      421 ggagcgcggc caggtggccg aggcgcgcaa ggtggtcgac acctatctcg gcgcgttccg
      481 ccccgatccc gacgtgctgt atgccgcggt gaacgtggcg cgtgccgcca aggacaagat
      541 gggcgaagag aaattctcgc gcacgctgcg cctggaattc ccggactcgg cgcaggcgcg
      601 tgcgctgaag cgcggttcct agagcgttcg atggacgaga cggccgccac tgcgactccg
      661 cccgcgccgc gttcgatcgg cgagcgcctg cgtgcgggcc gcgaacgctc cggcctgtcg
      721 attgccgccg ccgcggaaaa actgcacctc gacgccaagg tcatcgaggc gctcgaagcc
      781 gaccgtttcg cggaactggg cgcgtcggtg tacgtgcgtg gccaccttcg ccgttacggc
      841 gatttcgtgg gcgaggcggg cccggagctc gtgggcctgt atgccgctcg cgatgctcgt
      901 ccgccgccgc cggacctgac gcaaataccc caagccgagc gtcgcgccga tccacgccgg
      961 ctggtcactc ccttcgtagg cctgtcgtgc gcggccgtgc tgctcgtcgc catctggtgg
     1021 gtcctggcgg gttcgcgatc gggcagttcc gaagtcgcca cggcgctccc gactccggtc
     1081 gttcagcccg acatcgaagt cgccgcgaac actgcgccgg gcgtggcgac gcccgtgtcg
     1141 atggcgtcca gtacgccggt cactccgccc gccaccgcgc cggccggttc tgcggtcacg
     1201 acgccggcgg ccgccgcgca gcccaagcgg gaagaagtgg ctcccgcgcg cgagacacgc
     1261 ctgaagctcg atctcaccaa cgacagctgg gtcgaagtct acgattcgcg tggcgagcgg
     1321 ctgttcttcg acgtcgcgag tgccggcagc gtgcagagcg tgagtggccg cggtccgctg
     1381 cgcgtggtgc tgggccatgc cgccggagtc agtgtcgaag tcgacggcca gtcgcgcgag
     1441 attcccagcg ccgccatcga tggggaaagc gccagtttcg tcgtgaatcg ctccggctcg
     1501 ctttcgcgcg cccgctaaca aaagaaacca atgagccgaa ccattccgcc ggtcaccggc
     1561 atgaaagacg tccttgccgc cgacatcgcc ctctggcaac acgtcgaacg ctgctggcgc
     1621 accgtcgcgg atgcgtacgg ctaccaggag atgcgcgtgc ccgtggtcga gcacacggag
     1681 ctcttcaagc gcgccatcgg cgaatacacc gacgtggtcg agaaggagat gtacaccttc
     1741 accgaccagg gcggcgacag cctgacgctg cgtcccgaag ccacggccgg aatcgtgcgc
     1801 gcgacgatct ccaacggact gctgcgcggc gcgcggctca agctctggtg ccagggccct
     1861 atgttccggc atgaaaagcc gcagaaggga cggttccgcc agttcaacca gatcgatctc
     1921 gaggccatcg gcttcgccgg tcccgacgtc gacatcgaga tcatcgcgct caccgcgcgc
     1981 ctgtggaagt tgttaggcgt cacgcgcgtc aagctgcaga tcaattcgct gggcacgccg
     2041 gagtcgcgca aggcctaccg cacgctgctg caggactatt ttcgccaaca ccgggacgcg
     2101 ctggatgaag acggcaagcg ccgcctcgaa ggcaatccgc tgcgcatcct cgacagcaaa
     2161 cacccgccca cccaggccat cgtcggcaac gcgccggtgc tcacggagca tctcgacgcc
     2221 gaatcggccg agcatttcac gcagctgcgc gccgggctcg atgcgctcgg catcccgtac
     2281 acggtgaacc cgcgactggt acgcgggctc gactactact cgcgcacggt cttcgagtgg
     2341 atcaccgatg cgctcggcag ccaggacgcg gtctgctccg gcggccgtta tgacggcctg
     2401 atcgcgcaga tgggagggga cgccacgccc gccatcggtt gggccatggg cgtcgaacgc
     2461 gtggtcgaac tggtgcgcct
//
LOCUS       DATDEU010000697         2337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1157293, whole genome shotgun sequence.
ACCESSION   DATDEU010000697 DATDEU010000000
VERSION     DATDEU010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157293"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="VIV63_16720"
     CDS             complement(<1..1673)
                     /locus_tag="VIV63_16720"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6126298.1"
                     /translation="MFVIIGGVSFTRLPVAQYPEIAPPIINVSGQYPGASADVVASTV
                     VTPIEQQINGVENMIYMSSNSTADGRFSIAVTFDLGTNLDVAQVQVQNRVAIAQPRLP
                     VDVRNIGVTVTKSSPDLMMVVHLYSPDKSRDSLFISNYATLAITDTLTRVDGVGSITV
                     FGSRDYSMRVWLDPDRLQTVGLTAGDVVLALQAQNVQVAGGVLDQPPMPKQGAFQFAV
                     QTLGRLANPEEFANIVVKQAAGAVVRLKDVARIELAAQDYTSNSYLDRDPAVALAIFQ
                     RPGSNALATAKTIIATMDQLATRFPSGIARAIVYNPTEFIQESVNAVIETIGEAVILV
                     VLVVILFLQTWRAAVVPIVAIPVSLVGTFFFMGLFGFTLNNLSLFGLVLAIGIVVDDA
                     IVVVENVERNIESGLSPRDAAYKSMDEVGGALVAIALVLCAVFVPSAFITGISGQFYR
                     QFALTIASATIISLIVSLTLSPAMCALLLKRRPERHEEKWWERPIRGFFRGFNWGFDR
                     LGAGYHWLVARAVRYVVIMLVLYVGILAYGLNEFRKTPIGFIPQVDRGYL"
     gene            complement(1747..>2337)
                     /locus_tag="VIV63_16725"
     CDS             complement(1747..>2337)
                     /locus_tag="VIV63_16725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6126299.1"
                     /translation="GGATTLLANIVSTDPIRLEFTFDEASLLRYERLARDGNTDVTGR
                     GSAPVRLKLIDEKDFTHSGRMDFIDNVIDRSTGTIRGRAQFDNSDGLFTPGMFARVQV
                     PASAPYQALLVPDAAIGTEQVRKFVYVVDQDGVARQHFVTLGELVDDLRVIKEGVSAD
                     DRIVVNGLMRVRPGQKVTPEEQQTTPQAAAPQAKTN"
BASE COUNT          474 a          748 c          731 g          384 t
ORIGIN      
        1 agatagccgc ggtcgacctg cgggatgaac ccgatcggcg tcttgcggaa ctcgttgaga
       61 ccataggcaa gaatgccgac atagagaacg agcataatga cgacgtagcg cacggcgcgc
      121 gcgaccagcc agtggtaacc cgccccaagc cggtcgaagc cccagttgaa gccgcggaaa
      181 aacccgcgga tcggccgttc ccaccacttc tcttcgtgac gctccggtcg ccgtttgagg
      241 agcaaggcgc acatcgccgg cgacaacgtc aacgacacga tcagggaaat gatcgtcgcg
      301 cttgcgatgg tgagagcgaa ctgacgatag aactggccgg aaatgcccgt gatgaaggcg
      361 gagggaacga acaccgcgca cagcacaagc gcgatcgcga ccagcgctcc gccaacctca
      421 tccatgctct tgtaggccgc atcgcgcggt gacaaacccg actcgatgtt gcgctcgacg
      481 ttctccacga cgacgatggc atcgtcgacc acgatcccga tcgcgagcac cagcccgaac
      541 agcgaaaggt tgttgagcgt gaaaccgaac agccccatga aaaagaaggt gcccaccagc
      601 gaaactggaa tggcgacgat cggaacgact gccgcgcgcc acgtctgcag aaacaggatc
      661 accaccagca ccaccaggat gaccgcctcg ccgatggttt cgatcaccgc gttgacggat
      721 tcctggatga attcggtcgg gttgtagaca atcgcgcgcg cgatgccgga cgggaaacgc
      781 gtcgccagtt gatccatggt ggcaatgatg gtcttcgccg tcgccaatgc gttcgaaccg
      841 ggtcgctgga aaatggccaa tgccaccgcg gggtcgcggt cgagatagga attcgaggtg
      901 tagtcctggg cggccaactc gatgcgcgca acgtccttga gccggaccac cgcccccgcc
      961 gcctgcttca ccacgatatt ggcgaactcc tcgggattgg cgaggcggcc gagagtctgc
     1021 accgcgaact ggaatgcgcc ctgtttcggc atcggcggct ggtccagcac tccgcccgcg
     1081 acctggacgt tctgcgcctg caacgcgagc accacgtcgc ctgcggtcag gccgaccgtc
     1141 tgcagccggt cgggatcgag ccacacgcgc atcgagtagt cgcggctgcc gaacacggtg
     1201 atcgaaccca cgccgtcgac gcgcgtcagc gtgtcggtaa tcgcgagggt ggcgtagttc
     1261 gagatgaaca agctgtcgcg cgatttgtcc ggcgagtaga ggtgcaccac catcatcagg
     1321 tcgggcgagc tcttggtcac cgtgacgccg atgttgcgga cgtcgactgg aagtcgcggc
     1381 tgcgcgatcg caacccggtt ctgcacctgc acctgggcaa cgtcgagatt ggtcccgaga
     1441 tcgaacgtga ccgcgatcga gaaacgcccg tccgccgtcg agttcgacga catgtagatc
     1501 atgttctcga cgccgttgat ctgctgctcg atcggcgtca ccacggtcga ggcaaccacg
     1561 tccgcgcttg cgcccggata ctggcccgac acgttgatga tgggcggcgc gatctccggg
     1621 tactgggcaa cgggcaaccg cgtgaacgac acgccgccaa tgatcacgaa cacgatcgaa
     1681 acgaccgctg cgaaaatcgg acggtcgata aaaaaatgcg aaattcgcat cggcctctga
     1741 gcctgctcaa ttggtcttgg cttgcggggc ggccgcctgg ggcgtcgtct gctgctcctc
     1801 gggtgtcacc ttctgccccg ggcgcacccg catcaggcca ttgaccacga tgcggtcgtc
     1861 cgccgacacg ccctccttga tcacgcgcaa atcgtcgacg agttcgccca acgtgacgaa
     1921 gtgctgccgg gcgacgccat cctgatcgac gacgtagacg aacttgcgca cctgctcggt
     1981 accgatcgcg gcatcgggca ccaacagcgc ctgataaggc gccgaggccg gcacctgcac
     2041 gcgggcgaac atgccgggcg tgaacaggcc gtcggaattg tcgaactggg cgcgtccgcg
     2101 aatcgttccg gtcgagcgat cgatgacgtt gtcgatgaag tccatccggc cggagtgggt
     2161 gaaatccttt tcgtcgatca gcttcagccg caccggcgcg ctgccgcgcc cggtcacgtc
     2221 cgtgttgcca tcccgcgcca accgctcgta gcgcaacagc gacgcctcgt cgaaggtgaa
     2281 ttccagccga atgggatcgg tcgagacaat gttggcgagg agcgtggtcg ctccgcc
//
LOCUS       DATDEU010000698         8539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_134291, whole genome shotgun sequence.
ACCESSION   DATDEU010000698 DATDEU010000000
VERSION     DATDEU010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134291"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VIV63_16730"
     CDS             <1..495
                     /locus_tag="VIV63_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126300.1"
                     /translation="LEFTADTEHLPALLGAIQLPAEWPTETLHAAGELSWPADMQGDL
                     VHALSGKFDLETEGNDSTHQLSASATLADGQIALANVQGTGPEADQMFRGSGRVALLA
                     REYDLTVDYERVSLAASAVPTPARARVARAWTALRGSAARRGWTLEPESRRVQWHGTW
                     DAGQ"
     gene            complement(1188..1970)
                     /locus_tag="VIV63_16735"
     CDS             complement(1188..1970)
                     /locus_tag="VIV63_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY6126301.1"
                     /translation="MHRQAIGIDIGGSSIKAAVVDLHKGCLVGERHSVPSPAMSAVSE
                     MLHAMVAAVPEGTAQLAAGVTFPGVVVGGTVYSAANVAKSWIGQPLAEPAAAKLKRKV
                     VAINDADAAGLAEVQFGAARDVCGTVLVLSLGTGIGSALFLDGKLLPNTELGHLEFDG
                     MEAETRASGRARMERKLDWAAYMTELNYVLNRMHALFWPDLVVLCGGITADHPNLCDD
                     LRVPCKIKLGALRADAGIVGAAFATTLAEGVWINSAKEAVAK"
     gene            2165..4633
                     /locus_tag="VIV63_16740"
     CDS             2165..4633
                     /locus_tag="VIV63_16740"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY6126302.1"
                     /translation="MNEPTLVTGAILRRGSLRAAIYDRLTRSLGKEPETATPRDIYDA
                     LSLAVREELSARWLATQRRVARARVKRLCYLSVEFLPGRSLVNALSSLDSELIDEARV
                     AVASMGHDFDAVLALEVDPGLGNGGLGRLAACFLDSLATLQYPATGYGIRYDYGIFTQ
                     SIDTDGAQRESASTWLRLHNVWETPRSDARYIVRFGGRVLNTQDAGGVQRSRWVETRD
                     ILAVGFDQLVPGNRSPTVNHLRRWSGRALQPFHIEDFNAGNYSDAVEDQVDAKNLSRV
                     LYPDDSTPQGKELRFKQQYFFVSASIQDILATHLAEERALEDLPTALAVQMNDTHPTL
                     AIPELMRLLVDQYDMPWADAWAICRSVFGYTNHTLMPEALETWPVNFFERLLPRHLEI
                     IYRINEEFLLEVEKKFKGDDAAKTTLSIIAENHGRRVRMSHLAVVGSHKVNGVAKLHS
                     DLMKKTIFSGFARMFPERFTNVTNGITVRRWLKQSNPLLSRLLTERLGSAWENDLEEI
                     ERLRWAADDPHFRKQFRDIKRTNKLALAEEIDRRTGIDVSVDSLFDVHVKRIHEYKRQ
                     LLNILYVITRYNRIKANPDVHCVPRTVMFAGKAAPGYVLAKAIIRLINNVSTTINQDP
                     VTLTKLKCVFLPDYDVSLAQTIMPAADLSQQISTAGMEASGTGNMKLALNGALTIGTL
                     DGANIEIRDSVGPDNIFIFGMTADEAAQRQADGYRPREIVAANPELAATLEMIRGGAF
                     SRGGSDDARLLVDRLTSDGEPFLVLADFAAYCEAQDRVDALYRQPEEWSRRAVLNTVG
                     MGPFSSDRSVREYAERIWNIRSVI"
     gene            4787..6958
                     /gene="glgB"
                     /locus_tag="VIV63_16745"
     CDS             4787..6958
                     /gene="glgB"
                     /locus_tag="VIV63_16745"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY6126303.1"
                     /translation="MNVTHHEPRSLLGYHEFARGELPPLCVVRVLEPGAETVEVFWED
                     TPEELHPLKRIHDSGLFEGAIPQRRPVVPYRLRVRCPFDHVVVKHDTYFFSHEISEFD
                     LYLFGEGRHYGLYHKFGAHLRVRDGVAGTNFAVWAPNARRVSVVGSFNDWDGRKHAMQ
                     ARGGSGVWELFVPGVGEGAEYKYEIRTQRDSLLLKSDPFGFFMQKRPETASIVSSLEG
                     YEWQDAGWLRDRASRDWRRSPVNVYEVHLSSWQHSWDRKPPFFNWAEAIERLIPYVVD
                     MGYTHIELMGVAEHPFDGSWGYQVVGYYAPTSRYGTPKEFMAFVDACHQAGIGVILDW
                     VPAHFPKDAHGLAEFDGSSLYEHSDPRMGEHSDWGTKIFNYGRHEVKNFLVANALFWM
                     ERFHIDGLRVDAVASMLYLDYSRKAGEWLPNKYGGRENLEALEFLREFNTVAHREHPG
                     VLTIAEESTAFPGVSRPVHLGGVGFSFKWNMGWMNDTLSYFEKDPVYRRHEHNKITFS
                     FMYAWSENFVLPISHDEVVHGKKSLLDKMPGDEWQKRANYRLFMAYMTAHPGKKLMFM
                     GSEFGQWHEWREHEQLDWPLLQSPAHLGLQSLNRDLAKFYRHHPQLHASDCDAGGFRW
                     SDLHNADESVFAFLRQEHGHAPIVCVFNATPVPRDDYWLGVPEAGLYEVLFDSDYAGY
                     GGSGFSTVSGYVAFEHVVHGFSHALRIRLPPLAAVFLRRAG"
     gene            6958..>8539
                     /gene="glgX"
                     /locus_tag="VIV63_16750"
     CDS             6958..>8539
                     /gene="glgX"
                     /locus_tag="VIV63_16750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY6126304.1"
                     /translation="MKDGSPDILGATFTGDGVNFAVRSRAATRVDVCLYDSTGERETA
                     RLTLPAKTGAVHHGFVEKELARVGTLYGIRVHGVYDPREGYRFNANKLLIDPWARDIV
                     GDVKWDPSILGYDPADGDGLRPSLVDSAAAMPRCRVIDGVFDWEGDRQPAVPWRDSFI
                     YELHVKGFTQLHPQVPPEWRGKYLGLTVPAVIEHLKSLGVTAVELMPVHALTSEGFLH
                     ERGLTHSWGYNTLSWYAPTSRYAVDDPVVELKQAVKALHKAGIEVILDVVYNHTAEGN
                     HLGPTLNFRGIDNRAYYFHRANDRRFYDDVTGCGNTVACDHPIVQSDIVGSLRYFAEE
                     FRIDGFRFDLATVLGRDRSGFNSNSPFFSMLAADPVLAYVKLIAEPWDVGLGGYQLGN
                     FPPGWSEWNDRYRDTMRAFWHGGRRMLGGFAERFAGSSDLFRRNGRKPTASLNLVAAH
                     DGFTLRDTVSYNERHNEANLENNRDGHSNNHSWNCGVEGPTTDPAITELRTRQRRAML
                     STLLLARGVPLLLGGDELG"
BASE COUNT         1545 a         2776 c         2687 g         1531 t
ORIGIN      
        1 ctcgaattca ccgccgacac cgaacatctt ccggcgttgc tcggtgccat ccagttgccc
       61 gccgaatggc ccaccgaaac tctgcatgcg gccggcgagt tgtcgtggcc cgcggatatg
      121 cagggcgacc tggtacatgc gctcagcggg aaattcgatc tcgagaccga aggaaacgac
      181 agcacgcacc agctctccgc cagcgcgacg ctcgccgacg gccagatcgc gctcgccaac
      241 gtgcaaggca cgggtcccga agccgaccag atgttccggg gcagcgggcg cgtggcgctg
      301 ctggcccggg agtacgacct cacggtcgac tacgaacgag tgtcactggc cgccagcgcc
      361 gttcctacgc ccgctcgtgc gcgcgtagcg cgtgcctgga ccgcgttgcg aggttcggcc
      421 gcgcgccgcg gctggaccct ggagccggaa tcacgccggg ttcagtggca cgggacctgg
      481 gacgcgggtc aatgagggtg ttcgtgcgtg ccccagcgag cgcccccgcc cgagatgatg
      541 aggtcgctac caccgggctc tttgatctgt gggttggcgc cagattgcgg gccgccgctt
      601 caacgcgaag tcgtgtttct cgtggatgct gagacggtcg cgaagagtcc cgatgaagcg
      661 gagaagcggc ttcgtacgtt gtcagtccgc cagacccggc ccgctccctc atcatctcgt
      721 gcgggtgcgc tcgctggggc gccaccgtca ccgaaccgcg cccgatttcc cgctgcggtg
      781 atcggagaag agacgcacgg tcttgagctc ttcccacagc cgggtctaca cgcctctttt
      841 cctcgcgaac gaagctgggg ttcgttgcag tgagagtccg tcggggttcg gattcggagg
      901 gttcggcgct cgcgtgtgag cggcgcagcg cgagtcgcct cctggcgcgc agctcctctt
      961 tggtggttcc tccctacgaa gcggtatcag cccggcagtt atcccaccgc tgacagctct
     1021 ggcgcgccgc gactcgcgca agcgaccgcg aagaattcct cgcgtcccgc gaggaattct
     1081 tcaagcgcgc gaacccgcga gcgccgaacc ctccgaatcc gaaccccgac ggactctcac
     1141 tacgctgatc cccgtcccct gttgccagga aacgaagagt gtccccacta cttggcgaca
     1201 gcttccttcg cgctattgat ccacacccct tccgcgagcg tggtggcgaa cgccgcgccg
     1261 acgatgccgg catcggcgcg caacgcaccc agcttgatct tgcacggcac gcgcaggtcg
     1321 tcgcacaggt tcgggtgatc ggcggtgatg ccgccacaga gaacgacgag atccggccag
     1381 aacaacgcgt gcatgcgatt cagcacgtag ttgagttcgg tcatgtacgc ggcccagtcg
     1441 agcttgcgtt ccatgcgggc gcggccggag gcgcgcgtct cggcttccat tccatcgaac
     1501 tcgagatggc cgagctcggt gttgggcagg agcttgccgt cgagaaacag cgcactgccg
     1561 ataccggttc cgagactgag cacgagcacg gtcccgcaca catcgcgcgc ggcgccaaac
     1621 tggacttcgg caagacctgc ggcgtcggca tcattgatgg cgaccacttt gcgtttgagt
     1681 ttcgcggcag cgggttccgc cagcggctga ccgatccagc tcttggcgac gttggcggcg
     1741 gagtagaccg tgccaccgac gacgacgccg ggaaacgtaa cgcccgcggc gagttgggcg
     1801 gtgccttcgg gaaccgcggc gaccatggcg tgcagcattt cactgacggc ggacatggcg
     1861 ggcgagggca cggaatgccg ttcgcctacc aagcatccct tgtgcaggtc aacgacggct
     1921 gccttgatcg acgagccccc gatatcaata ccaattgctt gtcggtgcat tccgcgttaa
     1981 ctcgaagcca ttgtggtgca gcgcgaggaa tctgggcccg gttaccttga gtgactgtgc
     2041 ccgcccgcgc gaggtgcata tgataccgac acaacggctc cgacataggc tttgcatgca
     2101 atgtctggca cccaagaaaa atgtcctgaa atgtctcgtg tgcaactgaa aggtagttcg
     2161 agtgctgaat gagccgacgc ttgtgactgg cgcgatcctc aggcgtggat cgctgcgcgc
     2221 ggcgatttat gaccgcctga cgcgttcatt gggcaaagag cccgaaaccg cgacgccccg
     2281 tgacatatac gatgcgttat cgctcgccgt gcgggaggaa ttgtccgccc gttggctcgc
     2341 gactcagcgc cgtgttgcgc gcgctcgcgt caagcggctc tgttacctgt cggtggaatt
     2401 cctgcccggc cggtcgctgg tcaacgcact gtcgagcctc gattcggagt tgatcgacga
     2461 ggcgcgagtc gcggtcgcca gcatgggcca cgacttcgat gccgttctcg cgctcgaagt
     2521 ggatccgggc ctgggcaacg gtggcctcgg ccgcctcgcc gcgtgtttcc tcgactcgct
     2581 ggccaccctg cagtacccgg caacgggtta cggaatccgt tacgactacg gcattttcac
     2641 gcagtccatc gacaccgatg gcgcgcagcg cgaatccgcc agcacctggt tgcgactgca
     2701 caatgtgtgg gagacccccc gttccgatgc gcgatacata gtccgcttcg gaggccgcgt
     2761 gctgaacacc caggatgcgg gcggcgtgca gcgatcgcgg tgggtcgaaa cgcgtgacat
     2821 cctcgccgtc ggtttcgatc agctcgtgcc gggcaaccgc agtccgaccg tgaatcatct
     2881 gcgccggtgg tcgggccggg cgctgcagcc gtttcacatc gaggacttca acgccggcaa
     2941 ttactctgac gcggtcgaag accaggtcga cgccaagaat ctttcgcgcg tgctctatcc
     3001 ggacgattcg acgccgcagg gcaaggaact gcgcttcaag cagcagtact tcttcgtgag
     3061 cgcgagcatc caggacatcc tcgcgacgca tctcgccgag gaacgtgcgc tcgaagatct
     3121 gccgacggcg ctcgccgtgc agatgaacga tacccatccg acgctcgcga tccccgaact
     3181 catgcgcctg ctggttgacc aatacgacat gccgtgggcc gacgcctggg ccatctgccg
     3241 cagtgtgttc ggttacacca atcacacgtt gatgcccgag gcgctggaaa cctggcccgt
     3301 gaatttcttc gagcgcctgc tgccgcggca cctcgagatc atctaccgca tcaacgaaga
     3361 attcctgctc gaggtcgaaa agaagttcaa gggtgacgac gcggccaaga ccacgttgtc
     3421 gatcattgcc gagaaccatg ggcgtcgcgt gcgcatgtcg cacctggcgg tggtcggcag
     3481 ccacaaggtc aatggcgtcg ccaagctgca ttcggatctc atgaagaaga ccatcttctc
     3541 gggattcgcg cgcatgttcc ccgagcgctt caccaacgtg accaacggca tcacggtgcg
     3601 ccgctggctc aagcagtcga acccgctgct gtcgcggctg ctgaccgaaa ggctgggctc
     3661 cgcgtgggag aacgatctcg aggaaatcga acggttgcgc tgggcggcgg acgatccgca
     3721 cttccgcaag caattccgcg acatcaagcg caccaacaag ctggcgctgg cggaggagat
     3781 tgaccggcgc acgggcatcg acgtcagcgt cgattcgctg ttcgacgtgc acgtgaagcg
     3841 catccacgaa tacaaacgcc agcttctcaa tatcctgtac gtgatcacgc gctacaaccg
     3901 catcaaggcc aaccccgacg tccactgcgt tccgcgcacg gtcatgttcg cgggcaaggc
     3961 ggcgccaggt tatgtactgg cgaaggccat catccggctc atcaacaatg tatcgacgac
     4021 catcaaccag gacccggtca cgctgaccaa actgaagtgc gtgttcctgc cggattacga
     4081 cgtatcgctg gcgcagacca tcatgccggc ggccgatctc tcgcagcaga tttccaccgc
     4141 cggcatggaa gcgtccggca ccggcaacat gaaactggcg ctgaacggcg cgttgaccat
     4201 cggcacgctc gatggcgcca acatcgagat ccgcgattcg gtggggcctg acaacatttt
     4261 cattttcggc atgacggcgg acgaggctgc gcagcggcag gcggatggct accggccgcg
     4321 cgagatcgtc gccgccaatc cggagctcgc ggcgacgctc gagatgattc gtggcggcgc
     4381 attcagccgt gggggctcgg acgatgcccg gctgctcgtc gatcgcctga catccgacgg
     4441 cgagccgttc ctggtgttag ccgatttcgc ggcctattgt gaggcgcagg accgcgtcga
     4501 tgcactgtat cggcaacccg aagagtggag ccgccgcgcc gtgctcaaca ccgtcggcat
     4561 ggggccgttc tccagcgatc gcagcgtgcg cgagtacgcc gagcgcatct ggaacatccg
     4621 atcggtgatt taatggggac attcctgatt tgcagcaaat ggggacgcgc ctccccgagg
     4681 aatgctgaca aaatgcttgc caggtttgag gcgcgtcccc atttgctagc aaatcaggaa
     4741 tgtccccatt aagagcaagc tgacaaagac ggaaatcacc gccctggtga acgtgactca
     4801 ccacgagcca cgctcgctgc tgggttacca cgaattcgcg cgcggcgaat tgccgccgtt
     4861 gtgtgtggtg cgcgtgctcg agcccggcgc cgagaccgtc gaggtcttct gggaagacac
     4921 gcctgaagaa ctccatcccc tcaagcgcat ccacgactcc ggattgttcg aaggcgccat
     4981 tccgcagcgc cggccggtcg tgccgtaccg gttgcgcgtc cgttgtccgt tcgatcacgt
     5041 ggtcgtcaaa cacgacacct atttcttctc gcacgagatc tcggagttcg atctctatct
     5101 gttcggcgaa ggccggcact acgggctcta ccacaagttc ggcgcgcatc tgcgcgtgcg
     5161 cgacggcgtg gccggaacca actttgcggt ctgggcgccc aatgccagac gcgtgagcgt
     5221 ggtcggcagt ttcaacgatt gggacggccg caaacatgcg atgcaggcgc gcggcggctc
     5281 gggtgtgtgg gagctgttcg ttcccggcgt gggcgagggc gccgaataca aatatgagat
     5341 ccgcacgcag cgagactcgc tgctgctcaa gtccgatcct ttcggattct tcatgcagaa
     5401 gcgtccggag acggcgtcca tcgtctcctc gctggaaggc tacgagtggc aggacgccgg
     5461 ctggctgcgc gatcgcgcga gccgcgactg gcggcgttcg ccggtcaacg tatacgaagt
     5521 gcatctgtcg agctggcagc attcctggga tcgcaagccg ccgttcttca actgggccga
     5581 ggccatcgag cggctcattc cctatgtcgt ggacatgggg tacacgcaca tcgagctcat
     5641 gggtgtggcc gaacatccgt tcgacggttc ctggggctac caggtagtgg gctattacgc
     5701 gccgacctcg cgatacggca cgccgaagga gttcatggcc ttcgtcgacg catgccacca
     5761 ggccggcatc ggcgtgatcc tcgactgggt gcccgcgcat ttcccgaagg acgcgcacgg
     5821 cctcgcggag ttcgacggtt cgtcgctcta cgaacattcc gacccgcgca tgggcgaaca
     5881 cagcgactgg ggcacgaaga tcttcaatta cgggcgccac gaggtgaaga atttcctcgt
     5941 tgccaacgcg ctcttctgga tggagcgttt ccacatcgac ggattgcgcg tcgatgcggt
     6001 ggcgtcgatg ctctatctcg actacagccg caaggcgggc gagtggctgc ctaacaagta
     6061 cggcggacgc gagaatctcg aggcgctcga gttcctgcgc gaattcaaca ccgtggcgca
     6121 tcgcgaacac cccggtgtac tgaccatcgc cgaggagtcc acggcgttcc cgggcgtctc
     6181 gcgcccggtg catctcggcg gtgtcggctt ctcgttcaag tggaacatgg ggtggatgaa
     6241 cgacaccctg agctacttcg agaaggaccc ggtctaccgg cgccacgagc acaacaagat
     6301 cacgttctcg ttcatgtatg cctggagcga gaacttcgtg ctgcccatct cgcacgacga
     6361 agtcgtgcac ggcaagaagt cgctgctcga caagatgccc ggcgacgaat ggcagaagcg
     6421 cgcgaactac cggctgttca tggcgtacat gaccgcgcac ccgggcaaga aactcatgtt
     6481 catgggctcg gagttcggtc agtggcacga gtggcgtgaa cacgaacagc tcgactggcc
     6541 gctgctgcag agccccgcgc acctcggtct gcagtcactc aaccgcgatc tcgcgaaatt
     6601 ctatcggcac catccgcaac tccatgcgag cgactgcgac gccggcgggt tccgctggag
     6661 cgatctgcac aatgccgacg agagcgtgtt cgcattcctg cgccaggaac acgggcacgc
     6721 gcccatcgtc tgcgtgttca acgccacgcc ggtgcctcgc gacgactact ggttaggcgt
     6781 gcccgaggcc ggtctctacg aagtcctgtt cgattccgac tacgccggtt acggaggctc
     6841 gggattctcg accgtctccg gttacgtggc cttcgaacac gtggtccacg gcttctcgca
     6901 cgcgctgcgc attcgcctcc cgccgctggc cgcggtgttc ctgaggcgcg ctggctgatg
     6961 aaagacggtt cgcccgacat cctcggggcc accttcaccg gagacggcgt gaacttcgcc
     7021 gtgcgctcgc gcgccgcgac acgcgtcgac gtttgtctgt acgactccac cggcgagcgc
     7081 gagacggcgc ggctgaccct gccggcgaaa accggcgccg tgcaccacgg tttcgtcgaa
     7141 aaggaactgg cgcgcgtcgg cacgttgtat ggaatccgcg tgcacggggt ctacgacccg
     7201 cgcgagggct accgattcaa cgccaacaag ctgctgatcg atccctgggc gcgcgacatc
     7261 gtcggtgacg tcaaatggga tccatcgatc ctgggatacg acccggccga tggcgacgga
     7321 ctccgcccaa gcctcgtcga cagcgccgcc gccatgccgc gctgccgcgt gatcgacggt
     7381 gtcttcgatt gggaaggcga ccggcagccg gcggtgccct ggcgcgactc cttcatctac
     7441 gaactgcacg tcaagggatt cacgcagctc catccgcagg ttccgccgga atggcgcggc
     7501 aaatacttag gcctcaccgt gccggcggtc atcgagcacc tgaaatcgct cggcgtgacg
     7561 gcggtcgagc tcatgccggt gcacgcgctc accagcgagg gcttcctgca cgagcgcggc
     7621 ctcactcact cctggggcta caacacgttg tcgtggtatg cgcccacgag ccgctacgcg
     7681 gtcgacgacc cggtggtgga gctcaagcag gcggtcaagg ccctgcacaa ggcgggtatc
     7741 gaggtgatcc tcgacgtcgt ctacaaccac acggccgagg gcaatcacct cgggcccacg
     7801 ttgaacttcc gcggtatcga caatcgcgcg tactactttc accgcgccaa cgaccggcgc
     7861 ttctacgacg acgtgaccgg ctgcggcaac accgtggcct gcgaccaccc catcgttcag
     7921 tccgacatcg tcggcagcct gcgttacttc gccgaggaat tccgcatcga cggattccgc
     7981 ttcgacctgg ccacggtgct cgggcgtgac cggtcgggat tcaattccaa ttcaccgttc
     8041 ttctcgatgc tggccgcgga cccggtgctg gcctacgtga agctcatcgc cgaaccgtgg
     8101 gacgtcgggc tcgggggtta tcagctcggc aattttccgc cgggatggag cgagtggaac
     8161 gaccgttatc gcgacaccat gcgcgcgttc tggcacggcg ggcggcgcat gctcggcggc
     8221 ttcgcggagc gcttcgccgg atcgtcggac ctgttccggc gcaacggccg caagccgacg
     8281 gccagtctca acctggtggc cgcgcacgat ggcttcacgc tgcgcgacac ggtctcctac
     8341 aacgagcgcc acaacgaggc gaatctcgag aacaaccgcg acggccactc gaacaaccac
     8401 agctggaact gcggtgtcga aggacccacc accgacccgg cgatcaccga gctgcgcacc
     8461 cggcaacgcc gggcaatgct gtccaccctg ctgctggcca ggggagtgcc gttgttactc
     8521 ggcggtgacg agctcggcc
//
LOCUS       DATDEU010000699         3095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_865084, whole genome shotgun sequence.
ACCESSION   DATDEU010000699 DATDEU010000000
VERSION     DATDEU010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865084"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="glyQ"
                     /locus_tag="VIV63_16755"
     CDS             complement(<1..776)
                     /gene="glyQ"
                     /locus_tag="VIV63_16755"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY6126305.1"
                     /translation="MPSTLADMPLGTAPRTFQDLIFALQKYWAEQGCVILQPYDMEMG
                     AGTFHTATFLRSIGPEPWSAAYVQPSRRPTDGRYGDNPFRLQHYYQFQVVIKPSPLPI
                     LDLYLGSLRALGFDTRVHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVG
                     GLDCKPVTGEITYGLERLAMYLQGVESIFDIVWTDGPLGRVTYRDVFHQNEVEQSAYN
                     FEHADTAVLLRHFDDHEAACGKLLEAKLALPAYEQVMKAS"
     gene            complement(857..1549)
                     /locus_tag="VIV63_16760"
     CDS             complement(857..1549)
                     /locus_tag="VIV63_16760"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY6126306.1"
                     /translation="MVEVIHTVRAVRFAPCHRIVASRLPTIHLFERVADPADWDALYK
                     LETLTNPRLRQEVGELSLVPVADRVSGPNASVVMAPFTHLSPPGTRFTDGHFGAYYAA
                     ESVDTAISETRFHRENFLRATSQPPIELEMRCYLADVACDLHDLRSRRMEMPDVYDPA
                     SYAASQKLGRQLRDAGSNGIAFDSVRRNGGECVAIFRPRLVQNVRQSVHLRYAWDGNA
                     ISEVYEIRVVSL"
     gene            complement(1577..1990)
                     /locus_tag="VIV63_16765"
     CDS             complement(1577..1990)
                     /locus_tag="VIV63_16765"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEY6126307.1"
                     /translation="MSLTVANAVRNPSSGRSDVTSLGGAGLRAFVNIAEAWGLTVAEQ
                     LTLLGIASRSTFFKWRRERNPRLPRDTLERLSYLLGIYKSLQILLPDPLLADAWVRKP
                     NTATTFGGRSALERMLSGQVADLYVVRQYLDAERG"
     gene            2252..>3095
                     /locus_tag="VIV63_16770"
     CDS             2252..>3095
                     /locus_tag="VIV63_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEY6126308.1"
                     /translation="MKPADVIKLIKDKEVKYADLRFTDTRGKEQHVTIPANLVDEDLF
                     RDGKMFDGSSISGWKGINESDMILALDPSTAVLDPFFEQTTVNIRCDIIEPATMQGYE
                     RDPRSLGKRAEAYLKSTGIADGALFGPENEFFIFDSVRWNSGLNESSYSIDSVQGAWN
                     SNTVFPEGNKGHRPGLKGGYFPVPPVDAFQDIRGAMCDALEEMGLKVEVHHHEVATGG
                     QCEIGVGPGGLVKQADEVQILKYVLTQVGARYGKSVTFMPKPLVGDNGSGMHVHQSLQ
                     KNGVA"
BASE COUNT          630 a          947 c          968 g          550 t
ORIGIN      
        1 tgcgaggcct tcatgacctg ctcgtacgcc ggcagcgcga gtttcgcctc taacaacttg
       61 ccgcaggcgg cttcgtggtc gtcgaaatgc cgcagcagca ccgcggtgtc tgcgtgctcg
      121 aagttgtaag ccgactgttc cacttcgttc tggtgaaaga cgtcacggta ggtgacgcgg
      181 cccagcgggc catcggtcca gacgatgtcg aagatgctct cgacgccctg caggtacatt
      241 gcgaggcgct cgagtccgta ggtgatctcg cccgtgacgg gcttgcagtc gaggccgccc
      301 acctgctgga agtaggtgaa ctgcgtgacc tccatgccgt tgagccacac ctcccagccg
      361 agtccccagg cgccgagcgt cggcgactcc cagttgtctt cgacgaagcg gatgtcgtgc
      421 acgcgcgtgt cgaatccaag cgcgcgcaac gagcccagat agagatccag gatgggcagc
      481 ggcgatggct tgatgacgac ctggaactgg tagtagtgct gcaggcgaaa cgggttgtcg
      541 ccgtatcgac cgtcagtggg ccgtcgcgaa ggttggacgt acgcggccga ccacggctcg
      601 gggccgatgg atctcaggaa cgtcgccgta tgaaaagtgc cggcgcccat ctccatgtcg
      661 tacggctgca ggatgacgca gccctgttcc gcccagtatt tctgcagcgc gaatatcagg
      721 tcctgaaatg ttcgcggagc ggtgcccagc ggcatgtcgg caagtgtcga aggcattgtt
      781 cagttgagca ttttggccgg cgcgcagtat agcggggcac cccggaaaac tactgcagat
      841 tcagcgatcc gaagcgtcac aggcttacca cgcgaatctc gtagacctcg gagatggcgt
      901 tgccatccca cgcgtagcgc aggtgcacgc tctggcgcac gttctgaacg agccgtggcc
      961 ggaatatcgc gacacattcg ccgccgttgc gacgcacgct gtcgaacgcg attccattcg
     1021 aacccgcgtc gcgcaattgc cgccctagct tctgcgaggc cgcgtaactc gccggatcgt
     1081 agacgtcggg catctccatg cgccgggagc gcaggtcgtg caggtcgcag gcaacgtcgg
     1141 cgagatagca gcgcatctcg agctcgatcg gcggctggct ggtggcgcgc agaaaattct
     1201 cgcgatgaaa acgcgtttcc gagatcgccg tgtcgaccga ctcggcggcg taatacgcac
     1261 cgaagtgtcc atcggtgaaa cgcgtgcctg gcggcgacag gtgcgtgaac ggcgccatca
     1321 cgacggatgc gttgggtccg ctcacgcggt cggccacggg caccagtgac agctcgccca
     1381 cttcctggcg caggcgagga ttcgtcagcg tttcgagttt gtagagcgcg tcccaatccg
     1441 ccggatccgc gacccgctcg aacaggtgaa tcgtcggcag ccggctcgcg acaatgcgat
     1501 ggcacggcgc gaacctcacc gcccgcacgg tgtgaatgac ttcgaccatc cctggttcct
     1561 ctggcgtcag ccgccgttat ccacgctcgg catcgaggta ctggcgaacg acgtagaggt
     1621 cggcgacctg gccggacaac atgcgttcca gcgccgaccg gccgccgaac gtggtcgcgg
     1681 tgttgggctt gcgaacccat gcgtcggcca gtagcgggtc gggcagcaga atctgcaatg
     1741 acttgtagat gccgagcaga taggacaacc gttcgagggt gtcgcgcggc aggcggggat
     1801 tacgttcccg acgccacttg aagaaggtgg agcgcgaggc gatgcctaac aacgtgagtt
     1861 gttcggccac cgtcagcccc caggcttcgg cgatgttgac gaaggcgcga aggccggccc
     1921 cgcctaatga agtgacgtcc gacctgccgg aacttggatt gcggaccgcg ttggcgacgg
     1981 taaggctcat gaggcgctcc tgaagttcat atatggacta tacactccat acatccatat
     2041 agggacgaat tttcgcttca tgcgggtgcg atggacaaag ctggagcata tttcccgacc
     2101 aaataggcgc aaaatccccc gcgctcgcac caaagatgtg cgtgcccaaa gtcgaaatgc
     2161 aactaaacca ttgatttaga acgaatcagc ggctggcaca gcgaatgcat tgagaccgag
     2221 gcttaaaacc cttccgagag gatctctaac aatgaaaccc gcagatgtaa tcaagttgat
     2281 caaggacaag gaggtcaagt acgccgatct ccgcttcacc gacacgcgcg gcaaagaaca
     2341 acacgtaacg atccccgcga atctcgtcga cgaagacctt tttcgtgacg gcaagatgtt
     2401 cgacggctcg tccatttcgg gttggaaagg catcaacgag tcggacatga tcctggcgct
     2461 cgatccgagc accgccgtgc tcgatccgtt cttcgagcag accacggtca acattcgctg
     2521 cgacatcatc gagccggcga ccatgcaggg ttatgagcgc gatccgcgct cgctcggcaa
     2581 acgcgccgag gcctacctca agtcgaccgg catcgccgac ggcgcgctgt tcggccccga
     2641 gaacgaattc ttcatcttcg acagcgtgcg ctggaacagc ggcctcaacg agtcgtccta
     2701 ctcgatcgac tccgtgcagg gcgcgtggaa cagcaacacg gtattccctg aaggcaacaa
     2761 gggacatcgt cccggcctga agggcggcta tttcccggtt cccccggtcg acgccttcca
     2821 ggacatccgc ggcgccatgt gcgacgccct cgaagaaatg ggcctcaagg tcgaagtgca
     2881 ccaccatgaa gtggcgaccg gcggccagtg cgaaatcggc gtcggacccg gcggcctcgt
     2941 caagcaggcc gacgaagtgc agatcctgaa gtacgtgctg acgcaggtcg gcgcgcgcta
     3001 cggcaagtcg gtcacgttca tgccgaagcc cctggtcggc gacaacggtt cgggcatgca
     3061 cgtgcaccag tcgctgcaga agaatggcgt cgcgc
//
LOCUS       DATDEU010000700         6956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1010639, whole genome shotgun sequence.
ACCESSION   DATDEU010000700 DATDEU010000000
VERSION     DATDEU010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010639"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..824)
                     /gene="nth"
                     /locus_tag="VIV63_16775"
     CDS             complement(165..824)
                     /gene="nth"
                     /locus_tag="VIV63_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY6126309.1"
                     /translation="MNAKKRREIFERFRAANPHPTTELEYDTPFELLVAVILSAQATD
                     KSVNAATRKLFPVARTPQDIVKLGVDGLAKYIRTIGLWKGKAKNVIATASAIADEHGG
                     VVPRTRDELEKLPGVGRKTANVVLNTIFGEPTIAVDTHIFRVANRTRIAQGETPREVE
                     DALIKYTPGEFAHDAHHWLILHGRYVCKALKPECARCLVNKLCEYPLRHKIIAAARKA
                     P"
     gene            complement(835..1386)
                     /locus_tag="VIV63_16780"
     CDS             complement(835..1386)
                     /locus_tag="VIV63_16780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEY6126310.1"
                     /translation="MDNASAIDALLPQTQCTRCGYTGCLPYAEAIANGEADINQCPPG
                     GTETIVALAKLTGKSLSPLNREFGLEAAPKLAFIDEERCIGCTKCLPPCPVDAIVGAP
                     RRMHTVVAELCTGCELCVAPCPVDCIIMIPLLASPLPTLVIPPAEDSRTRYLAHNDRV
                     ARRAADRAATLAAKKRGARSPSP"
     gene            complement(1398..3437)
                     /gene="metG"
                     /locus_tag="VIV63_16785"
     CDS             complement(1398..3437)
                     /gene="metG"
                     /locus_tag="VIV63_16785"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY6126311.1"
                     /translation="MFRKILVTSALPYANGSLHLGHIIETVQTDIWVRFQRMQGHDCI
                     YVCAEDTHGTPVMIKAQAEGITPEELITRMAAEHRADYEGFLIGHDHFHSTHSPENKA
                     ITDDLYLKLKAAGHITTRSVRQAYDEKAGMFLPDRYVKGECPVCHTADQYGDSCENCG
                     STYTPDKLINPVSTISGTPPVLRESEHYFFKLGDFTEVLRQWLEGGGARQPEVRAKLA
                     EWFEAGLQDWDISRDKPYFGFEIPGAPGKYFYVWFDAPIGYLGSFKALCDKRGLNYDE
                     YLNAGSTTELYHFIGKDISYFHNLFWPAVLHGSGCRKPTAVFVHGFLTINGTKMSKTR
                     GTFITARKYLERLPPEPLRFYFASKLNSAVEDIDLSLEDFVARVNSDIVGKLVNIASR
                     CAGFVQRGGGKLAAELPDPQLYAEFAALRGTIADLYDQRDYSAAIRGIMGLADRANQY
                     VDSHKPWALAKDPARAAEVLAVCTQGINLFRVLMSYLAPVMPQMAEKAGKFLRISFAD
                     WNSVDTPLFDHPIEVYEPLATRLDPKIVAELIEPAAGAAAPAKTTEKAKKPVTAPPAA
                     PAAPGLVNIADFAKLDLRVAKVLDAKLVEGSDKLLQLTLDLGTEQRNVFSGIRSAYDP
                     ATLIGRQVVMIANLEPRKMRFGVSQGMVLCASGDDGPEVFLLSPDSGALPGMKVT"
     gene            complement(3481..4542)
                     /locus_tag="VIV63_16790"
     CDS             complement(3481..4542)
                     /locus_tag="VIV63_16790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY6126312.1"
                     /translation="MPVIDEYLKEVLSKKGSDLHFLAGDPPRIRLYGDLQPLRQEKLD
                     KEFVQNTLYEIMPKTAVKRFEEHDGADFAYNMGEYGRFRVNVMRQLNGMGAVMRAIPS
                     KALSMEQLNLPQACYSLCSANNGLILVTGKTGSGKSTTLAAMIDNINTRLKGHILTIE
                     DPIEFVHARKQCLVSQREVGVHTKGFAAALQSGLREDPDVVLVGELRDYETISIAVTA
                     AEMGILVMGTLHTNGAAPTVDRMINVFPADKQIHVRTMLSTSLRGVISQQLLQKADKS
                     GRVAALEILVNTPAVSNLIRQGKLDQLETTMQAGSAFGMRTMDNAIEALLNQRLITGK
                     EAYKKGISKAKFEPVKDQG"
     gene            complement(4554..5672)
                     /locus_tag="VIV63_16795"
     CDS             complement(4554..5672)
                     /locus_tag="VIV63_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY6126313.1"
                     /translation="MARIDAFLKLGMQQNCSDVHLAVGVPPLLRMNGDLMPIKFRELG
                     DAELESYLTEIFTKNQHEYFGKGNDLDFSYVSADGGRFRVNVYRKETGLGATFRAIPQ
                     DIPSIDKLALPPIVKKLCDYHQGMVLVTGATGTGKSTTLAAMIDHLNTSRRLNIVSLE
                     DPIEFVHRSKLSQVIQRELGTHIPTFAEGVRAAMREDPDVILVGELRDAETISMAMTA
                     AETGHLVLGTLHTTSAAKTIDRIIDALPIEEREQTKSFLSQSLLAVVTQILVKTADNR
                     SRKAICETLVMTRAIAKLIQTDQTHQIPNQIQTGRDLGMQLMDQALLTSLAAKEIDPD
                     DAVTYAADRRPFQRYVTDTTLMPKLDVTGPTSQAAGGG"
     gene            complement(5918..>6956)
                     /locus_tag="VIV63_16800"
     CDS             complement(5918..>6956)
                     /locus_tag="VIV63_16800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6126314.1"
                     /translation="TQASSTPAMSTAPSVPKGGRPAADAPPPPPPAPPPAPTSQQART
                     QLMSEQDVQRAVKEAEKMAGPQKLDIQTLKTGDIIEGRYKYVERIGKGAFGTVLLMED
                     TVVDERLILKFLNPNVSEDEEIMKRFVHELRYSRKITHHNVIRIYDFLYIQGNYAISM
                     EYFPSHTLGSEVVGEKPMPLPRALGFGIDICTGMEVAHAQGIVHRDLKPANVLINNEG
                     LLKIVDFGVAAAQREGDTQLTKTGYVIGSPKYMAPEQILGKKVDQRADVYALGVILYE
                     IMTGVPPYARGDHMSVMYQHVQGKARPPREINPGLPPGLPEVIMQAMSVDKDKRYQTM
                     PDLRAALEKFR"
BASE COUNT         1137 a         2037 c         2347 g         1435 t
ORIGIN      
        1 gaggtcggcc gccgccgatt ccacgatgac gtggtattca gggtgctcgg cgatcaccgc
       61 gatgacctgc ttgtcgagcg gcgacagcgt gtgctggtgc ttgtaccggt gccacgccgc
      121 gcggtaggtc tcgcgcagct ggtcgcgcga ctggccggcg aaaatcacgg agccttcctc
      181 gccgcggcga tgatcttgtg ccggagggga tactcgcaca atttgttcac gagacagcgt
      241 gcgcattctg gcttcaacgc tttgcagaca taacggccgt gcaggatgag ccagtgatgt
      301 gcgtcatgag cgaattcccc gggcgtgtac ttgatcaatg cgtcttccac ttctcgcgga
      361 gtttcgccct gggcgatcct cgtgcggttg gcgacgcgga aaatgtgagt gtcgacggct
      421 attgtcggct cgccgaagat tgtgttcagt accacgttcg cagttttgcg acccactccc
      481 ggaagttttt cgagttcgtc gcgagtacgc gggacgactc ccccgtgttc gtcggcaatc
      541 gcagacgctg tcgcgatcac gttcttcgcc ttgcctttcc acaggccgat cgtgcggatg
      601 tacttcgcga ggccatcgac gccgagtttc acaatatcct gtggagtacg ggcaaccgga
      661 aacagcttgc gcgttgcggc attgacgctc ttgtcggtgg cttgcgccga gaggatgacg
      721 gccaccaata gctcgaacgg cgtgtcgtat tcgagctcgg tcgtgggatg cgggttcgcc
      781 gcccggaatc gctcgaagat ttcgcgacgc tttttggcgt tcatggagcc gcgttcatgg
      841 acttgggctg cgcgcgccac gcttcttcgc ggccaatgtc gcggcgcgat ccgccgcgcg
      901 gcgggccacg cgatcgttgt gggcgagata cctcgtccgc gagtcttcgg ccggcggaat
      961 cacgagcgta ggcagcgggc tcgccagcag cgggatcatg atgatgcaat ccacgggaca
     1021 gggcgcgacg cagagctcac accccgtaca cagttcggcc accacggtat gcatgcgccg
     1081 tggggcgccg acgatggcat ccacggggca gggcggcagg cacttcgtgc aaccgatgca
     1141 gcgctcttcg tcgatgaagg ccagcttggg cgcggcttcg agtccgaatt cccggttgag
     1201 cggtgacagt gacttgccgg tcagcttcgc caacgcgacg atggtttcgg tgccgcccgg
     1261 cgggcattga ttgatgtcgg cctcgccgtt cgcgatggct tcggcatacg gaagacagcc
     1321 cgtgtagccg cagcgcgtgc actgggtctg cggcagcagg gcatcgatgg cggacgcgtt
     1381 gtccacgcgc ggacccctca ggtgactttc attcccggca gcgcgccgct gtccggcgac
     1441 agcaggaata cttcggggcc gtcatcgcca ctggcgcaca acaccatgcc ctgcgaaacg
     1501 ccgaagcgca tcttgcgcgg ctcgaggttg gcgatcatga ccacctggcg gccgatcagt
     1561 gtcgcggggt cgtaagcgga gcggatgccc gagaacacgt tgcgctgttc ggtgccgaga
     1621 tcgagcgtga gctgcagtag tttgtccgag ccttcgacga gtttcgcgtc gagtactttg
     1681 gcgacgcgca ggtcgagctt ggcgaagtcg gctatgttga cgaggccggg ggcggccggg
     1741 gcagcagggg gagcggtcac gggtttcttc gccttctcgg tggttttggc gggcgcagcc
     1801 gcacctgcgg caggctcgat caactcggcc acgatcttgg gatcgagacg cgtcgccagc
     1861 ggttcgtaga cttcgatcgg atggtcgaac agcggcgtgt ccaccgagtt ccagtcggcg
     1921 aacgagatgc gcagaaactt cccggccttt tccgccattt gcggcatgac cggcgcgaga
     1981 tagctcatga gcacgcggaa cagattgatg ccctgcgtgc agacggccag gacttccgcg
     2041 gccctggccg gatccttggc gagcgcccag ggcttgtgcg aatccacgta ctgattggcg
     2101 cgatcggcga ggcccatgat gccgcggatc gccgccgagt agtcgcgctg atcgtagagg
     2161 tcggcaatcg tgccgcgcag cgccgcgaac tcggcgtaca actgcggatc gggtagttcg
     2221 gccgccagct ttccgccgcc gcgctgcacg aatcccgcgc aacggctggc gatgttcacg
     2281 agcttgccga cgatgtcgga gttcacgcgc gcgacaaaat cttctaacga caagtcgatg
     2341 tcttccaccg ccgaattcag cttgctcgcg aaatagaagc gcagtggttc ggggggcagg
     2401 cgttcgagat acttgcgcgc ggtgatgaac gtgccccgcg tcttcgacat cttggtgccg
     2461 ttgatcgtga ggaagccgtg cacgaacacc gcggtcggtt tgcggcaacc cgagccatgc
     2521 agcaccgcgg gccagaacag gttgtggaag tagctgatgt ccttgccgat gaagtgatac
     2581 agctcggtag tgctgccggc gttcagatat tcgtcgtagt tcaggccgcg cttgtcgcac
     2641 aacgccttga agctgccgag atagccgatg ggcgcgtcga accacacgta gaaatatttg
     2701 ccgggggcgc ctgggatctc gaagccgaag tagggcttgt cgcgcgagat gtcccagtcc
     2761 tgcaggcccg cttcgaacca ctcggcgagc ttggcgcgca cttcgggctg gcgcgcgccg
     2821 ccgccctcga gccactgccg cagcacctcg gtgaaatcgc cgagcttgaa gaagtagtgc
     2881 tccgactctc gcaacaccgg cggcgttccg ctgatggtcg aaacaggatt gatgagcttg
     2941 tcgggcgtgt aggtagaccc gcagttctcg cacgaatccc cgtattgatc cgcggtatgg
     3001 cagaccgggc attcgccttt cacgtagcgg tcgggcagga acatgccggc cttctcgtcg
     3061 tacgcctggc gcacgctgcg cgtggtgatg tgcccggcgg ccttgagctt caggtacaga
     3121 tcatccgtga tcgccttgtt ctcgggcgag tgcgtcgaat gaaagtgatc gtgaccgatc
     3181 aggaaccctt cgtagtcggc gcgatgctcg gccgccatgc gcgtgatcag ttcttcgggc
     3241 gtgattccct cggcctgggc cttgatcatc accggcgtgc cgtgcgtgtc ttccgcgcac
     3301 acgtagatgc agtcgtgtcc ctgcatgcgc tggaagcgca cccagatgtc ggtctgcacg
     3361 gtttcgatga tgtggccgag atgcagcgag ccgttggcgt acggcagcgc actggtgacg
     3421 aggatcttgc gaaacatgaa aaccgcgggt ttctgaaggg cccgcaagta tagacgaggg
     3481 ttaaccctga tctttgaccg gctcgaactt ggccttggaa atgccctttt tgtaggcttc
     3541 cttgcccgtg atgaggcgct ggttgagcag cgcttcgatc gcgttgtcca tggtgcgcat
     3601 gccgaacgcg ctgccggcct gcatggtggt ttccagctgg tcgagcttgc cctggcggat
     3661 gaggttggac accgccggtg tattgacgag gatctcgagg gctgccacgc ggccgctctt
     3721 gtcggccttc tgcaaaagct gctgcgagat cacgccgcgc agcgacgtcg ataacatcgt
     3781 gcggacgtgg atttgtttgt cggccgggaa gacgttgatc atgcgatcga cggtcggcgc
     3841 ggcgccattg gtatgcagcg tgcccatgac gaggatgccc atctcggcgg ccgtgaccgc
     3901 gatgctgatg gtttcgtaat cgcgcaactc gccgaccagc accacgtcgg gatcttcacg
     3961 aagtcccgat tgcagcgcgg ccgcgaagcc cttggtgtgg acgccgactt cgcgctggct
     4021 gaccaggcac tgcttgcgcg cgtgcacgaa ttcgatggga tcctcgatgg tgaggatgtg
     4081 gcccttgagc cgcgtattga tgttgtcgat catcgccgcg agcgtggtcg acttgcccga
     4141 gccggtcttg ccggtcacga ggatgagccc gttgttggcg gagcacagcg agtaacacgc
     4201 ctgcggcagg ttgagctgct ccatcgacag cgccttggaa gggatggcgc gcatgaccgc
     4261 gcccatgcca ttcagctggc gcatgacgtt gacgcggaag cgcccgtatt cacccatgtt
     4321 gtaggcgaag tcggcgccgt cgtgttcctc gaaccgcttc accgcggtct tcggcatgat
     4381 ttcgtacagc gtgttctgca cgaactcctt gtcgagcttt tcctgacgca gcggctggag
     4441 gtcgccatac aggcggatgc gcggcggatc gccggccagg aagtgaaggt ccgagccttt
     4501 cttgctcagc acttccttga ggtattcgtc gatgaccggc atgcccgtgt tccctaaccg
     4561 ccgcccgctg cctgggaggt ggggccggtg acgtcgagtt ttggcatcag cgtggtgtcg
     4621 gtgacgtagc gctggaacgg gcggcgatcg gccgcgtacg tgaccgcgtc gtcgggatcg
     4681 atttccttgg cggccagcga ggtgagcagc gcctgatcca tgagttgcat gccgaggtcg
     4741 cggccggtct ggatctggtt cggaatctgg tgtgtctggt cggtctggat gagcttggcg
     4801 atggcgcgcg tcatgacgag cgtttcgcag atggctttgc gcgagcggtt gtcggcggtc
     4861 ttcaccagga tctgcgtgac cacggccagc aaactctgcg acaggaagct cttggtctgt
     4921 tcgcgttctt cgatcggcag cgcgtcgatg atgcggtcga tggtcttcgc ggccgaggtg
     4981 gtgtgcagcg tgcccagcac caggtggccg gtttccgcgg cggtcatcgc catggagatg
     5041 gtttcagcgt cgcgcaattc gccgaccagg atcacgtccg ggtcttcgcg catggcggcg
     5101 cgcacacctt cggcgaaggt cgggatgtgc gtgccgagtt cgcgctggat gacctgcgag
     5161 agcttgctgc gatggacgaa ttcgatgggg tcttcgagcg acacgatgtt gaggcgccgc
     5221 gacgtgttca ggtgatcgat catcgccgcg agcgtggtcg atttgcccgt gcccgtggcg
     5281 ccggtgacca gcaccatgcc ctggtgatag tcgcagagct tcttgacgat cggcggtaac
     5341 gccagcttgt cgatgctcgg gatgtcctgc ggaatcgcgc ggaaggtggc gccgaggccg
     5401 gtttccttgc ggtacacgtt gacgcggaag cggccgccgt cggcggacac gtaagagaag
     5461 tcgagatcgt tgcccttgcc gaaatactcg tgctgattct tcgtgaagat ttcggtgaga
     5521 taactctcga gctccgcatc gccgagctcg cgaaacttga tgggcatgag gtcgccgttc
     5581 atgcgcagca gcggcggaac gccgacggcc agatgcacgt cggagcaatt ctgctgcatg
     5641 ccaagtttca ggaacgcgtc tatgcgggcc acgggggcga agacctcaag ttatgtcgtt
     5701 aactcgccca agcatgcgac ggcgagaagg gattcaagtg tgacgcagtg cgcgattttc
     5761 cgcgaggcgt tccggccctg gcattaccgc aagcgaatac tgaggggtat gcctcaggca
     5821 aagaatgggg acattcctga tttgctagca aaaggggacg cgcctgaagc aggcacgtcc
     5881 ccttttgccg cttcgcgggc aaatcaggaa tgtcccctca gcggaatttt tcgagagccg
     5941 cgcgcaggtc aggcatggtc tggtagcgct tgtccttgtc gaccgacatg gcctgcatga
     6001 tgacttcggg caagcccggt ggcaggcccg ggttgatctc gcgcggcgga cgcgccttgc
     6061 cctggacgtg ctgatacatc accgacatgt ggtcgcctcg cgcgtacggc ggcacacccg
     6121 tcatgatttc gtaaaggatg acgccgagcg cgtacacgtc ggcgcgctga tccactttct
     6181 tgccgaggat ctgttcgggc gccatgtatt tcggcgatcc gatgacgtag ccggtcttcg
     6241 tgagctgcgt gtcgccttcg cgctgcgcgg ccgcgacgcc gaagtccacg atcttgagca
     6301 ggccttcgtt gttgatgagc acgttcgcgg gtttgaggtc gcgatgcacg atgccctgcg
     6361 cgtgcgcgac ttccatgccc gtgcagatgt cgatgccaaa gccgagcgcg cgaggcagcg
     6421 gcatcggctt ctcgccaacg acttcgctgc caagcgtgtg cgaggggaag tactccatcg
     6481 agatggcgta gttgccctgg atgtacagga agtcgtagat gcggatgacg ttgtggtgcg
     6541 tgatcttgcg cgagtagcgc agctcgtgca cgaaacgctt catgatctct tcgtcttcag
     6601 agacgttcgg gttcaggaac ttgaggatga gacgctcgtc gaccacggtg tcttccatga
     6661 ggagcaccgt gccgaacgcg cccttgccga tgcgttcgac gtacttgtaa cgcccttcga
     6721 tgatgtcgcc ggtcttgagc gtctggatgt cgagcttctg cgggcccgcc atcttctcgg
     6781 cttccttcac cgcgcgttgt acgtcctgct cggacatgag ctgggtgcgc gcctgctgcg
     6841 acgtcggtgc tggcggcggc gccgggggag gtggcggcgg tgcgtccgcg gcgggacgac
     6901 cgcctttcgg aacgctgggc gccgtcgaca tcgccggcgt gctcgaggcc tgggtg
//
LOCUS       DATDEU010000701         2436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_574841, whole genome shotgun sequence.
ACCESSION   DATDEU010000701 DATDEU010000000
VERSION     DATDEU010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574841"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VIV63_16805"
     CDS             <1..384
                     /locus_tag="VIV63_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY6126315.1"
                     /translation="TIFAGWYYWFPKMSGYMYSETIGKLHFWLTFVGVNIVFFPQHFL
                     GLAGMPRRIADYPDAFAGWNLVSSLGSYLSGFGALVFFFGVAQAFMRKERAADNPWGA
                     GATTLEWTLPSPPPFHQYNELPLIK"
     gene            457..1410
                     /locus_tag="VIV63_16810"
     CDS             457..1410
                     /locus_tag="VIV63_16810"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY6126316.1"
                     /translation="MSLLVSEGAEFTAGSVVSAGHEPSIASVGDYVALMKPRVMSLVV
                     FTALVGLVIAPGHMHPVIAFTALLCIAVGAGAAGALNMWYDADIDAVMARTSNRPIPQ
                     RRIAPGEALAFGIVLAAFSVGTLGLLVSWLAAGLLAFTVFFYIVVYTAWLKRSTPQNI
                     VIGGAAGAFPPMIGWAAATGGISLEPWLLFLIIFFWTPPHFWALSLTRTEDYARARIP
                     MLPVVAGGTETRRQILLYSIMLVPVGVAPWLFGYADAVYGITALVAGLFMVAFAWRVR
                     NEREGARADRAAHHLFAFSILYLFVLFAVLLVEGGRGGFGG"
     gene            1425..1583
                     /locus_tag="VIV63_16815"
     CDS             1425..1583
                     /locus_tag="VIV63_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126317.1"
                     /translation="MDEKPPTIVLSEQQKRSRRARSIAIALALGALVILFYVVTLVKG
                     PGVLNRPL"
     gene            1589..2188
                     /locus_tag="VIV63_16820"
     CDS             1589..2188
                     /locus_tag="VIV63_16820"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY6126318.1"
                     /translation="METPNTSSQRRNHIIVATACGVFVAAMVGAAYAAVPLYNWFCRT
                     TGFGGTTQVATAAPSHILDRKLKIRFDANVAGGLPWRFEPEQNSIEVRIGEVVTVSYS
                     VVNEAARETVGVASYNVSPPTAGIYFSKINCFCFTDQRLTGGEKRDMAVVFFVDPELV
                     KDWEQDGLDTITLSYTMYPARQPEAPRVGSGAALAPGRS"
     gene            2250..>2436
                     /locus_tag="VIV63_16825"
     CDS             2250..>2436
                     /locus_tag="VIV63_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY6126319.1"
                     /translation="MADAHAKPQHDYHLVQPSPWPALGSVSAFVAAVGGITWMHHMFP
                     AAPYIFGVGVIGILYTML"
BASE COUNT          375 a          769 c          781 g          511 t
ORIGIN      
        1 accatctttg ccggctggta ctactggttt ccaaagatga gcggctacat gtactcggag
       61 acgatcggca agttgcactt ctggctcacc ttcgtcggcg tcaacatcgt cttcttcccg
      121 caacacttcc tcgggctcgc cggcatgccg cggcgcatcg ccgactatcc cgacgcgttc
      181 gctggctgga acctggtctc ctcgctcgga tcctaccttt cggggtttgg cgcgctggtg
      241 ttcttcttcg gcgtcgccca ggcgttcatg cgcaaggagc gggcggcaga caatccatgg
      301 ggggcaggcg ccaccacgct cgaatggacg ctgccttcgc cgcctccgtt ccaccaatac
      361 aacgagttgc cgctgatcaa atgagcgcgg cgacgtccca ttggcggtgg acttggcgcc
      421 attgggtagg tcatcagaca gctgagtaca ggatcgatgt cgctactggt aagtgaaggc
      481 gcagagttca cggcaggcag cgtggtttcg gccggccacg agccgagcat cgcgagcgtc
      541 ggcgactatg tggcgctgat gaagccccgg gtcatgtctc ttgtcgtctt cacggcgctg
      601 gtcggcctcg tcatcgcgcc cgggcatatg catcccgtga tcgccttcac ggcgctgctg
      661 tgtattgcgg tcggggcggg tgcggccggc gcgctcaaca tgtggtacga tgccgacatc
      721 gatgcggtca tggcgcgcac gtcgaaccgc cccatcccac agcgccgcat cgcacccggg
      781 gaagcgctgg ccttcggcat cgtgctcgct gcattctcgg tcggcacgct cggcctgctg
      841 gtgagttggc tggcggccgg tctgcttgca ttcaccgttt tcttttacat cgtcgtgtat
      901 acggcatggc tcaagcgctc cacgccgcag aacatcgtta tcggcggggc ggcgggtgcg
      961 ttccccccca tgatcggctg ggctgcggcc accggcggca tttcgctcga gccgtggctg
     1021 ctgtttctca tcatcttctt ctggacgccg ccccatttct gggcgctgtc gcttacgcgc
     1081 accgaagact atgcccgcgc gcgcatcccg atgctcccgg tcgttgccgg gggaacggag
     1141 acgcggcggc agatcctgct ttattcgatc atgctggtgc cggtcggagt cgcgccgtgg
     1201 ctgttcggct atgccgacgc ggtctatgga atcaccgcgc tggtggccgg gcttttcatg
     1261 gtcgcgttcg cttggcgcgt gcgcaacgag cgtgaaggtg cacgcgccga ccgcgcggcg
     1321 caccatcttt tcgctttttc gatcctttat ctgttcgtgc tcttcgcagt gctgctggtc
     1381 gagggcgggc gcggcggctt cggcggttga ggcctggtga tgatatggac gaaaagccac
     1441 cgacaatcgt ccttagcgag cagcagaaac gcagtcgccg cgcgcggtcg atcgcgattg
     1501 cgctggcgct cggggccctt gtcatcctgt tctatgtcgt cacgctggtg aagggaccgg
     1561 gcgtgctgaa ccgtccgttg tgatcgcgat ggaaaccccg aacacgtcct cccagcggcg
     1621 caaccacatc atcgtcgcga cggcttgcgg tgtgttcgtc gcagccatgg tgggagcggc
     1681 atatgcggcc gtgccactgt ataactggtt ctgccgcacg actggcttcg gtggaaccac
     1741 ccaggtcgct accgccgcac cctctcacat tctcgatcgc aagctcaaga tccgtttcga
     1801 tgccaacgtc gccggcgggc tgccttggcg tttcgagccc gagcagaatt caatcgaggt
     1861 caggatcggc gaggtcgtga ctgtgagcta cagcgtcgtc aacgaagcgg cgcgcgagac
     1921 cgttggcgtt gcctcctaca acgtctcgcc gccgacggcg ggcatctatt tctcgaagat
     1981 caactgcttt tgctttaccg atcagcggct cacgggtggc gagaagcgcg acatggcggt
     2041 cgtgttcttc gtggatcccg agctggtgaa ggattgggag caggacggtc tcgacaccat
     2101 cacgctgtcc tacaccatgt atccggcgcg tcagcccgag gcgccgcggg tcggtagcgg
     2161 ggcggcgctt gcgcccggac gaagctgacg taataaggga atgaccgcca gggccccacg
     2221 tggcccacgg agagacggag agatccaaga tggccgacgc tcacgccaag ccgcaacacg
     2281 attatcacct ggtccagccc agcccgtggc cggcgctcgg ttcggtttcc gcgttcgtgg
     2341 cggcagtcgg gggcatcacc tggatgcacc acatgttccc ggcggcacca tatattttcg
     2401 gtgtcggcgt gatcggcatt ctctacacga tgctcg
//
LOCUS       DATDEU010000702         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1593953, whole genome shotgun sequence.
ACCESSION   DATDEU010000702 DATDEU010000000
VERSION     DATDEU010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593953"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VIV63_16830"
     CDS             <1..739
                     /locus_tag="VIV63_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6126320.1"
                     /translation="IGRKKIVLAGCLLAALTYFPIFKGITHFANPALDHAVQTSPVYV
                     MTNPDGCSFQFDPVGKKQFKTGCDLAKGALARMGVSYTIGDDSLSRDIDATVVVGQES
                     IEVKDLHGTLTAAQYKKAQDEVSAGIKELLVKAGYPAKADMEAFNYPMVILLLTILVL
                     YVTMVYGPIAAWLVELFPANIRYTSMSLPYHIGNGWFGGFLPVTSFAIVAATGNIYNG
                     LWYPVIVAAITIVIGGLFLRETKDSPA"
     gene            818..>2261
                     /locus_tag="VIV63_16835"
     CDS             818..>2261
                     /locus_tag="VIV63_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY6126321.1"
                     /translation="MKLSRGWIAAALVAAGTASAAVPTLEAFFDGASIQSVSISPDGQ
                     LLAMIVTTEDKIFVAVKDRKSSTPALPVLAPNGKDGFEPRWCRWANNERLVCSFLGHE
                     RHKYVGKVFPVTRLVSVNKDGTKQKQLLQNPFAPSGQLNDRIIDWTPESPETVLIEKY
                     HPRVGLRVLELNIYSGESRPYESPHEHIGGFGTDGHGNVRLGWGVVDMKTYYQARLHG
                     EKKWRILARVNTLSTDEAFNPIAVIPGTNYAYATRDHEGRSALWKIDLADKEDPLLVF
                     ASSRVDISPVFTPDNRLLAVLPDSGSKDAYYIEPAAELLGEVLAKLFKDRVYHVVDMS
                     ADLKTVVVVSESDVRAPEYHVLDLSTTPAAMQRVGSRYPGLEKADLATTKYLTYPARD
                     GKAIPAFLTKPVTAGPSLPPLIVMPHGGPWARDAWGFDSWVQVLAREGYAVLQMNYRG
                     SGGYGKEWREASLQNWGGLPYSDTIDGLKWA"
BASE COUNT          424 a          711 c          727 g          399 t
ORIGIN      
        1 gatcggccgc aagaagatcg tgctcgcggg ttgtttgctg gcggcgctca cgtacttccc
       61 gatcttcaag ggcatcacgc atttcgcgaa tccggcgctg gatcacgccg tccagacttc
      121 gcccgtctac gtgatgacga atccggatgg atgcagcttc cagttcgatc cggtcggcaa
      181 gaagcagttc aagaccggct gcgatctcgc caagggcgcg ctcgcgcgca tgggcgtctc
      241 gtacacgatc ggggacgatt cgctcagccg cgatatcgat gccacggtgg tggtcggcca
      301 ggagtccatc gaagtgaagg acttgcatgg cacgttgacc gcggcccaat acaagaaggc
      361 gcaagacgag gtctcggcgg ggatcaagga gttgctggtg aaggcgggct atcccgcgaa
      421 ggccgacatg gaagccttca actacccgat ggtgatcctg ctgctgacga tcctcgtcct
      481 gtacgtgacg atggtctacg gccccattgc ggcctggctc gtggagcttt tccccgcgaa
      541 cattcgctac acctccatgt cgctgccgta tcacatcggc aacgggtggt ttggcggctt
      601 cctcccggtc acatcgttcg ccatcgtcgc ggccacggga aacatctaca acggtctgtg
      661 gtatccggtc atcgtcgcgg cgatcacgat cgtcatcggc ggtctgttcc tgcgggagac
      721 caaggactcg cccgcatagc agaacccgat cgacaagacc cccgcgttta tgcaacatcg
      781 ggcgggtcta gttagtcggg gggtggttgt gcggcgaatg aagctttcgc gcggctggat
      841 tgccgctgcg ctcgttgcgg ccggcacggc cagtgccgcc gtgcccacgc tcgaggcgtt
      901 cttcgacggc gcgtccatcc agtcggtgtc catttcgccg gacggccagc tgctcgcgat
      961 gatcgtcacc accgaagaca agatcttcgt ggcggtcaag gatcgcaaga gctcgacgcc
     1021 cgcgctgccg gtgctcgcgc ccaatggcaa ggatggtttc gagccgcgct ggtgccgctg
     1081 ggcgaacaac gaacgcctgg tgtgcagttt cctcggccac gaacgtcaca aatacgtcgg
     1141 caaggttttc ccggtcactc gcctggtctc ggtgaacaag gacggcacga agcagaaaca
     1201 gcttctccag aatccgttcg cgccgagcgg ccagctcaac gaccgcatca tcgactggac
     1261 gcccgagtct cccgagaccg tgctcatcga gaaataccat ccgagagtcg ggttgcgcgt
     1321 gctcgagctg aacatctaca gcggggagag ccggccctac gagtcgccgc acgaacacat
     1381 cggcggattc ggcaccgacg ggcacggcaa cgttcgcctg ggctggggtg tcgtcgacat
     1441 gaagacctac taccaggcgc ggcttcacgg cgaaaagaag tggcgcatcc tggctcgcgt
     1501 gaacacgctg tccacggacg aagccttcaa tccaatcgcg gtgattcccg gcacgaacta
     1561 cgcttacgcc acgcgggatc acgaaggccg ctcggcgctg tggaaaatcg acctggccga
     1621 caaggaagat ccgctgctgg tcttcgcgag ctcgcgcgtc gacatctcgc cggtgttcac
     1681 gcccgacaat cgactgctcg cggtgctgcc cgacagcggc agcaaggacg cgtactacat
     1741 cgaacccgcc gcggagttgt taggcgaggt gctcgcgaag ctgttcaagg atcgcgttta
     1801 ccacgtcgtc gacatgagcg ccgacctcaa gaccgtggtg gtggtcagcg aaagcgacgt
     1861 gcgcgcgccg gaatatcacg tgctggacct gtccaccacg ccggcggcca tgcaacgcgt
     1921 cgggtcgcga tatcccggac tcgagaaggc cgacctggcc acgacgaaat acctgacgta
     1981 cccggctcgc gatggcaagg cgatccccgc gttcctgacc aagccggtga cggcgggccc
     2041 ttcactgccg ccgctgatcg tcatgccgca cggaggtccc tgggcgcgcg acgcatgggg
     2101 attcgatagc tgggtgcagg tgctggcgcg cgaagggtac gcggtgctgc agatgaacta
     2161 ccgcggctcg ggcggctacg gcaaggagtg gcgtgaagcg tcgctgcaga actggggcgg
     2221 gctgccatac agcgacacca tcgacggcct caagtgggcc a
//
LOCUS       DATDEU010000703         2145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1885460, whole genome shotgun sequence.
ACCESSION   DATDEU010000703 DATDEU010000000
VERSION     DATDEU010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885460"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VIV63_16840"
     CDS             complement(<1..1037)
                     /locus_tag="VIV63_16840"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY6126322.1"
                     /translation="MTEDTGAEARYRQMFEALIEGFCSIEMVFDERGKAVDYRFLEVN
                     PAFERHTGVANARGRLMRDIAPDLESNWFELFGKVATSGEPACFINEARPLGRIYEVN
                     AYRIGGKGSLQVGILFNDITDRRNSERKLRAQFERMNLLHQITRAVGEHQDMPSIFQV
                     VIRTLEDQMPVDFGCICLYDEAANDVMVASVGLRGSELATELTRTENQHVNIGDECVA
                     KIVLGQLIYEPETRAVEWPFSQRLAKGDLRAMVAVPMMIEGKLFGVLVVARNAPNSFS
                     SGECEFLTHLSEHVAQAAQHAQLHSALHGAYEDLRDTQAAILQQERLRALGQMASGIA
                     HDINNAISPISL"
     gene            complement(1139..1864)
                     /locus_tag="VIV63_16845"
     CDS             complement(1139..1864)
                     /locus_tag="VIV63_16845"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY6126323.1"
                     /translation="MNGALTLGAALLLGLAASGHCLVMCGGISAALGMAASKDSKGRT
                     RPAIMLAYQLGRIFSYALAGLLLAGIFGGLVSLLDIEWVRRTLRAMSALALLLGALVA
                     FGTLRDPGGGIGRKLWPRIAPLGRRLLPVNTLPRGFAFGMVWGWMPCGFVYSVLLIAA
                     LQLDAANGALIMTAFGLGTAPALFIAAFGAQQFQRFASRPAARHIGGSVLLVSAMLTL
                     AGPWIEHSLPGLHAWLPFDCGVR"
     gene            complement(1861..2016)
                     /gene="ccoS"
                     /locus_tag="VIV63_16850"
     CDS             complement(1861..2016)
                     /gene="ccoS"
                     /locus_tag="VIV63_16850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY6126324.1"
                     /translation="MNILLLLIPLSLLVLAGAVWVFFWAVNHDQFEDLDTPALLPLDD
                     LDRRETK"
BASE COUNT          405 a          692 c          680 g          368 t
ORIGIN      
        1 agcgaaatcg gcgagatggc gttgttgatg tcgtgcgcga tgccgctcgc catctgtcct
       61 agcgcgcgca gacgctcctg ctggaggatg gcggcctgcg tatcgcgcag atcttcgtag
      121 gcgccgtgga gcgcgctgtg cagctgggca tgttgcgccg cctgcgcgac gtgttcgctc
      181 aggtgcgtca ggaactcgca ctcgccgctg ctgaagctgt taggcgcgtt tcgcgccacc
      241 accagcacac cgaacaactt gccctcgatc atcatcggta cggcaaccat tgcgcgcaaa
      301 tcgccttttg cgaggcgttg cgagaatggc cattcgaccg cgcgcgtctc cggctcatag
      361 atcagttgcc cgagcacgat cttggcgacg cactcgtcgc cgatgttcac gtgctggttc
      421 tcggtgcgcg tcagctcggt tgccagttcg ctgccgcgca ggccgacgct cgccaccatg
      481 acgtcgttgg ccgcttcgtc gtacagacat atgcacccga aatcgacggg catctggtcc
      541 tcgagcgtgc ggatcacgac ctggaagatg ctcggcatgt cctggtgctc accgaccgcg
      601 cgcgtgatct ggtgcagcag gttcatccgt tcgaactgcg cgcgaagctt ccgctcggaa
      661 ttcctccggt cggtgatgtc gttgaaaagg atgccgacct gcaggcttcc tttaccgccg
      721 atgcgatagg cattgacttc gtagatgcgg ccgagcggcc tcgcttcatt gatgaagcac
      781 gcgggctcac ccgaggttgc gaccttgccg aacaactcga accagttcga ttcgaggtcg
      841 ggcgcgatgt cgcgcatcag ccggccgcgg gcattcgcca cgccggtgtg cctttcaaaa
      901 gcgggattga cctcgaggaa gcgatagtcg accgctttgc cgcgctcgtc gaacaccatc
      961 tcgatgctgc agaacccttc gataagggct tcgaacatct ggcgatatcg ggcttccgcc
     1021 ccagtgtcct cggtcatccc ggtcgaggtt agttgcgccg caacggaaag gcaaagcgac
     1081 taacaggctt gcgccgggca tgtaggtgag actcgcagca acttcccttt gtcctacgtc
     1141 agcggacgcc gcaatcgaac ggcaaccatg catgcaggcc gggcaaggaa tgctcgatcc
     1201 acggccccgc cagcgtgagc atcgcgctga ccaggagcac cgaaccaccg atgtgccggg
     1261 ccgccggtcg ggacgcaaac cgctgaaact gctgggcgcc gaaggcggcg atgaacagcg
     1321 ccggcgcggt gccgagtccg aacgccgtca tgatgagcgc gccattcgcg gcatcgagct
     1381 gcagggcggc tatcaacagc acggagtaca cgaagccaca gggcatccag ccccacacca
     1441 tgccgaaggc gaaaccgcgc ggcagcgtgt tgaccggcag aagtcgccgg ccgagcggcg
     1501 cgatgcgcgg ccagagcttt cgtccgatac cgccgccggg gtcgcgcagg gttccgaagg
     1561 caaccagcgc acccagcagc agcgcgagcg cggacatggc gcgcaatgtt cgccgcaccc
     1621 actcgatgtc gagaaggctc acgagcccgc cgaaaattcc ggcgagcagc agacccgcga
     1681 gtgcatacga aaagatccgg ccaagctggt aggccagcat gatcgcgggc cgcgtgcgtc
     1741 cctttgaatc tttcgatgcc gccatgccga gcgccgcgga tattccgccg cacatgacga
     1801 ggcaatgacc gctggcggca aggccgagca gcaacgcggc gccgagtgtc agcgcgccat
     1861 tcatttcgtc tcgcgcctat ccagatcgtc gagcggcagc aacgccggcg tgtccagatc
     1921 ttcgaactga tcgtgattga ctgcccagaa aaatacccag acggcgccgg cgaggaccag
     1981 cagcgacagc ggaatgagca acagcaggat gttcatgcga tcgccggtcg cgtggaggtt
     2041 gccggagtcg acttcggttt acgtgccgcg tgctgatcgg cgtcgccgcg cccgatgcgc
     2101 agcgcgttga ggatgactcc cagcgaactc aacgacattc cagcg
//
LOCUS       DATDEU010000704         4361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1302888, whole genome shotgun sequence.
ACCESSION   DATDEU010000704 DATDEU010000000
VERSION     DATDEU010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302888"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VIV63_16855"
     CDS             complement(<1..1108)
                     /locus_tag="VIV63_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126325.1"
                     /translation="MERASKFALAGAIAVMASALVPTPVRADTVRHSFVRAEWRDLGT
                     LGGSYSAAKDINERLEIVGWGRIPSGHKHAFKFSAGVMTDLDPTSFESVANAINDSSI
                     VVGARRLAGEPLFQPATYAAGGMRLLRVPTPSGGCSWGGEATDINDTNRTVGIGRAFG
                     TACDPSFATDGLWWKTSSSTSAPATVLGYPGSAELLRINNAGLAVGRFVVGGLYQPMS
                     WRPGILRPLTLPTEPVAFDAGKPTAINDGGVIAGFSLLPTYRFGGRAATRWITDMSPG
                     EVFAIPPADSREWAPFDINNSNFMVGDEEGTPWIYHQDFGLVFLPFPAGRECNVVGMT
                     EVIAGRIYAVGDCGTTSVNTRATIWGIQIRTVSLA"
     gene            1367..2365
                     /locus_tag="VIV63_16860"
     CDS             1367..2365
                     /locus_tag="VIV63_16860"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6126326.1"
                     /translation="MTRRVRPLNSPVLLMLIAAAFATLSACTDGSMSAAADKERRLVL
                     GFSPIVSWGGWSGANADSVRNAARDANMEVLMEDTRYRQENQVATLRSFIRQRVDVIV
                     FAPVVESGWDLVLREIRTAGIPVILMDRNIEVSDASLYVSHVGSDFVEEGRRAGRWLL
                     EHTKGETGEIAILELRGSDGSAPANDRKLGFSQAIAGDARYRIVESQNGNFFRPQARD
                     ITAKYLAVEGNRVRVIFAHNDGMALGGIEAVEAAGLKAGSDVLVIAIEGSREGLEAIV
                     AGKLNLSVECNPLVGPQLIAVARDAAAGKQVPRRVVTEETVFTRENAVAELPKRTY"
     gene            complement(2387..2671)
                     /locus_tag="VIV63_16865"
     CDS             complement(2387..2671)
                     /locus_tag="VIV63_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126327.1"
                     /translation="MDQEQALVQIVENHAEISGELMKGFPSPDRPGFLVLTIRVDAAH
                     AIANWPNLFARDVGSTIEVLAREGSQAALTPPGPVRLKVRKAGLSTSFAE"
     gene            complement(2671..3531)
                     /locus_tag="VIV63_16870"
     CDS             complement(2671..3531)
                     /locus_tag="VIV63_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126328.1"
                     /translation="MPHAVFNQRLSMHYDLELRCNGSGFTGSHSLGGSARPLGEITGI
                     YARMMDPDALPELRATPRSLPDQESVAKARAVCALFDEVMEVLAVPVVNRPSCMGSNL
                     SKPAQAQAIVRAGLLTPPTLITNVPDAVRAFHADHRRIVYKSISAARSIVREWTPEAG
                     PDIAAVAHLPTQFQAFIPGQNVRVHVIGEQVFATAIDSDAVDYRYAGRDDAEVHMQPL
                     QLPTFVAEACSRVTSDLGLTLSGIDLKRTQTDEWYCFEVNPSPAYSFFEELGGQPIAA
                     ALVQLLSGRA"
     gene            complement(3578..>4361)
                     /locus_tag="VIV63_16875"
     CDS             complement(3578..>4361)
                     /locus_tag="VIV63_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126329.1"
                     /translation="TATAVESALRRRGDFEVERASMDDLARASWRHELNPRGRVTTAH
                     RFGARASADLDVVFNRLVPATSHFNRWNVTDRQYAHSELLAFVLSWLESLGDRVVNRP
                     VCGNLVGPMDKPWLWLARAHKAGLLLHPSGATTSTRRFPAPRESVERVELLPASGATH
                     INRPVGFAARPEALVEVLVVGDRIVRGHPAQAAHEACRRLARLAGTDVLSVRLSLLPN
                     DAEWHFVDANAVPALEDDESLDALATLLALRARPHDSIRRHS"
BASE COUNT          784 a         1426 c         1381 g          770 t
ORIGIN      
        1 gcgccaatga caccgtacga atctggatgc cccaaatcgt cgctcgcgta ttcacggacg
       61 tcgtcccgca gtcgcccacg gcatagatgc gaccggcaat cacttccgtc atccccacta
      121 cgttgcattc tcgtccggcg ggaaagggca ggaacaccag gccgaagtcc tgatggtaga
      181 tccacggggt tccttcttca tcgcccacca tgaagtttga attgttgatg tcgaagggcg
      241 cccattcacg actgtcggcg ggcggtatgg cgaacacctc gccgggactc atgtcggtga
      301 tccaccgtgt cgccgcgcgc ccaccgaagc ggtaggtagg aagcagcgag aacccggcga
      361 tgactccccc atcattgatg gcggttggct tgcccgcgtc gaaggccacc ggctcggttg
      421 gcagagtcag cggccgcagg atgcccggcc gccacgacat gggttgatac agtccgccca
      481 ccacgaaccg accgacggcc agtccggcat tgttgatgcg aagaagctcc gcagagcccg
      541 gatatccgag caccgttgcg ggtgcgctgg tgctgctgct ggtcttccac caaagtccat
      601 cggtggcaaa actcggatcg cacgcggttc cgaaagccct cccaatcccc accgttcgat
      661 tcgtgtcgtt gatgtcggtg gcctctccac cccacgagca accgccggaa ggtgtgggca
      721 cccgcaagag gcgcatccca ccggccgcat acgtggccgg ctgaaacaac ggctcgccag
      781 ccaggcgccg cgcacccacc acaatggacg agtcgttgat cgcgttagcc acactctcga
      841 acgaggtggg atccaggtcc gtcatcacgc cagcactgaa cttgaaggca tgtttgtggc
      901 cggaaggtat gcggccccac cctacgatct ccagtcgttc gttgatgtcc ttggcggcgc
      961 tgtagctgcc gcccaaggtg cccaggtcgc gccactctgc tctcacgaac gagtgccgca
     1021 ctgtgtcggc gcgaacgggt gtcgggacca gggccgatgc catgacggca atagctccgg
     1081 ccagcgcgaa cttgcttgca cgttccatgg tttctccatg ccttgccgaa aggcaggcag
     1141 tcggacagta cgccacccat gccggcgttc taccgggacc tgagcgaacg tccctgacac
     1201 tgtcgattag ttccgccgaa actgcatgcc cccggggttc tttgtcttct ctagctagac
     1261 gccgagcgtc tcgcattccg cccctgccgc ccgactcagt ggctctccgc ggcccgccag
     1321 gtctccacct ctcgacgcct tccccgccag agcccatcat cgggccatga cacgccgcgt
     1381 tcgaccgctg aactccccgg ttctgctgat gctgatcgcg gcggcgttcg ccaccctctc
     1441 ggcctgcacg gacgggtcca tgagcgcagc cgccgacaaa gagcgccggt tggtgctcgg
     1501 cttcagcccg atcgtgagct ggggtggttg gagcggggcc aacgcggatt cggtccgcaa
     1561 tgcagcccgc gacgccaaca tggaagtgct catggaagat acgcgctatc gccaggagaa
     1621 ccaggtggcg acgttgcgat ccttcattcg acagcgcgtc gacgtgatcg tgttcgctcc
     1681 ggtggtcgaa tccggctggg acctcgtgct cagggaaatc cggaccgcgg gaattcccgt
     1741 gatcctcatg gatcgcaaca tcgaggtgag cgacgcctcg ctgtacgttt ctcacgttgg
     1801 ctcggacttc gtcgaggaag gacggcgggc cgggcgctgg ctgctggaac acaccaaagg
     1861 cgaaacgggt gagatcgcca ttctggaatt gcgcggttcg gatggctcgg cgccggcgaa
     1921 cgaccgcaag ctggggttct ctcaggccat cgccggcgac gcgcgttatc gcatcgtcga
     1981 atcacaaaac ggcaacttct ttcgccccca ggcgcgtgac atcacggcca aatacctggc
     2041 ggtcgagggc aaccgcgtcc gcgtcatctt cgcgcacaac gacggcatgg cgctgggcgg
     2101 catcgaggcc gtcgaggccg cgggcctcaa agccggcagc gacgtcctgg tgattgccat
     2161 cgaaggcagt cgcgagggtc tcgaggccat cgtggccggc aagctcaatc tgagcgtgga
     2221 gtgcaatcca ctcgtcgggc cgcagctgat cgccgtcgcc agggatgccg ccgccggcaa
     2281 acaggttccg cgccgcgtgg tcacggaaga gactgtgttc actcgcgaga acgcggtggc
     2341 ggaattgccg aagcggactt actgaaaggt cgacactgct tctcgcctac tccgcaaacg
     2401 acgtcgacag gccggctttg cgcaccttca accggaccgg gccggggggc gtcagtgcgg
     2461 cctgactacc ttcgcgcgcc agcacttcga tggtgcttcc cacgtcgcga gcaaacaggt
     2521 tgggccagtt cgcgatcgca tgtgcggcat caacccggat cgtgagtacg aggaatcccg
     2581 gccgatccgg gctggggaaa cccttcatca gctcacccga aatttcggca tggttctcga
     2641 cgatttgcac cagggcctgt tcctgatcca tcacgctcta cccgagagga gctgcacgag
     2701 cgcggcggcg atgggttgtc cgccgagttc ttcgaagaaa ctatacgccg gggacgggtt
     2761 cacttcgaag cagtaccact cgtccgtctg cgtgcgcttg agatcgatgc ccgacagcgt
     2821 gagacccagg tcactggtca ccctcgagca cgcctcggcc acgaaggtcg gaagctgcag
     2881 cggctgcatg tgcacttccg catcgtcgcg ccctgcatag cgatagtcga ctgcgtcgga
     2941 gtcgatcgcc gttgcgaaaa cctgctctcc aatgacgtgc acacgcacgt tctggcccgg
     3001 aatgaaggcc tggaactggg tcggaagatg cgcgaccgcg gcgatatcgg ggcctgcctc
     3061 gggagtccac tcgcgcacga tcgagcgagc ggcggagatc gacttgtaga cgatgcggcg
     3121 gtggtcggcg tgaaaagcgc gtaccgcatc cggtacgttt gtgatcagcg tcggcggtgt
     3181 cagaagtccc gcacgcacga tggcctgcgc ctgggcgggt ttcgagagat tgctgcccat
     3241 gcaagatggc cggttgacga cgggaacggc caacacctcc atcacctcat cgaagagcgc
     3301 acacacggcc cgggctttcg cgacagattc ctggtccggt agcgagcggg gtgtcgcgcg
     3361 aagttccgga agcgcgtccg ggtccatcat gcgtgcgtag atgccggtga tctcgcccag
     3421 tggtctggcg ctgccgccca gcgaatgact tccggtgaat ccactcccgt tgcacctgag
     3481 ctccaggtca tagtgcatcg acaggcgctg attgaagacg gcgtgcggca agccggcgcg
     3541 ctccgccgcc tctatcgcga gccggagagg cgcttcgcta ggaatgccgg cgaatagaat
     3601 catggggtct tgcgcgtagc gcgagtagcg ttgcgagagc atccagggac tcgtcgtctt
     3661 cgagcgcggg caccgcgttt gcatccacga aatgccactc ggcatcattc ggcaggagtg
     3721 acagacgcac actcaggacg tccgtccctg caagtcgtgc caggcgacga caagcttcat
     3781 gcgccgcttg cgccggatgg ccacgcacga ttcgatcgcc gaccacgaga acctcgacca
     3841 gtgcctccgg cctcgcagcg aagccgaccg gtcgattgat gtgtgttgcg ccggatgcgg
     3901 gcagtagttc gacgcgctcc acggattccc ttggcgccgg aaagcgccgc gtgctcgtcg
     3961 tggcgccgct cggatgcaga agcagtcccg ctttgtgcgc gcgcgcaagc cagagccagg
     4021 gtttgtccat cgggccgacg aggtttccgc acacgggccg attcacgacc cgatcgccca
     4081 ggctttcgag ccagctcagg acgaaggcca gtagttcgct gtgcgcgtac tggcgatccg
     4141 tgacattcca gcgattgaag tgcgaagtcg cgggcacgag ccggttgaac acgacgtcca
     4201 gatcggcaga cgctcgcgcg ccgaaccgat gtgccgtcgt gacccgccct cgcgggttca
     4261 gctcatggcg ccagctcgcg cgggcgagat cgtccatcga cgcccgttcg acctcgaagt
     4321 caccgcgccg gcgcagcgcc gactcgacgg ccgtcgccgt c
//
LOCUS       DATDEU010000705         2623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1449716, whole genome shotgun sequence.
ACCESSION   DATDEU010000705 DATDEU010000000
VERSION     DATDEU010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449716"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VIV63_16880"
     CDS             <1..1524
                     /locus_tag="VIV63_16880"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY6126330.1"
                     /translation="LSEAARLDVDVARTAHEFAAEGFALPYGDSALLNSSWAYRNAPY
                     VVAQNTGAFLEIPSTLDEQHTVEKPADADAYLARMEAYAGQLDGETGRLKSAAAQNVI
                     APDFLLDKTLHQIRLARSGNAADWSLVASLAKHAKDLPGNFGARAAKLAADKVAPALD
                     RQIAELDAHRKRANSDAGVWKLPRGDEYYAWVLRAGTTTRMTPEEIHQRGQEELRALQ
                     SEMDSILKKQGMSQGTVGERMTALGKQDRFRFEESDAGRAKLMAFLNGRVTDIRTRLP
                     RAFETLVPGNFEIKRMPPEVEPGAPGAYGGPGTIDGKVPGKFWINMHTMSIWTTYNTP
                     TLTYHESLPGHVWQGEYTFKLPLIRSLLAFNAYSEGWALYAEQLANELGAYDDDPFGR
                     LGYLQSIAFRACRLVVDTGLHAKRWTRQQAIDWFASTNGSSVEEVTGEVDRYCAWPGQ
                     ACGYKVGHSEINRLRTKAQAAMGARFNFRKFNDAVVTGGGVPMLTLARSIDAFIAKK"
     gene            1587..2282
                     /locus_tag="VIV63_16885"
     CDS             1587..2282
                     /locus_tag="VIV63_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6126331.1"
                     /translation="MIKLHDVHKYYRTGEQSLHVLKGIDLHIREGELVSIMGSSGSGK
                     STLLNILGVLDGFDRGSYVLAGHPVNNLKETEAARLRNKLLGFVFQSFNLLPFKNAAE
                     NVALPLYYQGVGRKERNYRAEQFLDMVGLSDRKSFMPNQLSGGQKQRVAIARALVANP
                     KVILADEPTGQLDSKSTEEIVALLKDIHRRGNTMIIVTHEQDIADQTERQIILKDGVA
                     TSTQLAERQAETN"
     gene            2285..>2623
                     /locus_tag="VIV63_16890"
     CDS             2285..>2623
                     /locus_tag="VIV63_16890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6126332.1"
                     /translation="MLDGLQEIFFTLRQNKLRTLLTAFGVFWGIFMLVLLLGAGRGMQ
                     NGVYEDFGSDVLDFIIVWTGTTTTAYRGMPPGRAIRLNLEDIEAVRRQISGIRLLSAE
                     RFVPDGGTINY"
BASE COUNT          542 a          838 c          820 g          423 t
ORIGIN      
        1 ctcagcgaag cagcgcgcct cgacgtcgac gtcgcgcgca ctgcgcacga attcgccgcc
       61 gaaggcttcg ccctgcccta cggcgactcg gcgctgctca attcgagctg ggcatatcgc
      121 aacgcgccct acgttgtcgc acagaacacc ggcgcgttcc tggaaatccc gagcacgctg
      181 gacgagcagc acaccgtgga gaaacctgca gacgcagatg cctatctcgc gcgcatggag
      241 gcctacgctg ggcagctcga tggggaaacg gggcggctga aaagcgctgc ggcgcagaac
      301 gtgatcgcgc cggacttcct gctcgacaag acgctgcatc agatcaggct cgcgcgcagc
      361 ggcaacgcgg ccgactggtc gctggtggcg tcgctcgcga agcacgcgaa agacctgccc
      421 ggcaatttcg gggctcgcgc cgcgaaactg gcggctgaca aagtcgcgcc ggcgctcgac
      481 cgccagatcg ccgaactcga cgcgcatcgc aagcgcgcca acagcgacgc cggcgtctgg
      541 aagctgccgc gcggcgatga gtactacgcc tgggtgttgc gcgcgggcac gacgacgcgc
      601 atgacgccgg aagagattca ccagcgaggc caggaagaac tgcgcgcgct gcagtccgaa
      661 atggacagca tcctgaagaa gcagggcatg agccagggga cagtcggcga acgcatgact
      721 gcgctcggca aacaggatcg cttccgcttc gaggaaagcg atgccggccg cgccaagctc
      781 atggccttcc tcaacggacg tgtgaccgac atacggacga gactgccgcg cgcgttcgag
      841 acgctggtgc ccgggaactt cgagatcaaa cgcatgccgc ccgaggtgga gccgggcgca
      901 cccggcgcgt acggcggtcc gggcaccatc gacggcaagg tgccgggcaa gttctggatc
      961 aacatgcaca cgatgagcat ctggacgacc tacaacacgc cgacgctcac ctatcacgaa
     1021 tcgcttcccg gccatgtctg gcagggcgag tacaccttca aactaccgct gatccgctcg
     1081 ctgctcgcgt tcaacgcgta ttcggagggc tgggcgctct acgccgaaca gctcgccaac
     1141 gagctgggcg cttacgacga cgatccgttc ggacgcctcg ggtatctgca atccatcgct
     1201 tttcgcgcgt gccggctggt ggtcgatacg gggctgcatg ccaaacgctg gacccgccag
     1261 caggccatcg actggttcgc cagcaccaat ggatcgagtg tcgaagaggt taccggcgaa
     1321 gtggatagat actgcgcctg gcccggccag gcctgcgggt acaaggtcgg gcacagcgag
     1381 atcaaccggt tacgcacgaa agcccaggcc gccatgggcg cgcgtttcaa cttccgcaag
     1441 ttcaacgacg cggtggtgac cggcggtggc gtgccgatgc tgacgctggc ccggtcgatc
     1501 gacgccttca tcgcaaaaaa atgatcggtt gatgcaaccc aaacgccccg gcgcgcgtct
     1561 gcatggactg gtgccctgaa aaaaacatga tcaagctgca cgacgtacac aagtactacc
     1621 gcaccgggga gcaatccctg cacgtgctca agggcattga cctgcacatt cgcgagggcg
     1681 agctggtctc catcatgggc tcgtccggct ccgggaaatc gacgctgctc aacatcctcg
     1741 gtgtgctgga tggctttgac cgcggcagct acgtcctcgc cggtcacccg gtcaacaacc
     1801 tgaaggaaac cgaggccgcc agacttcgca acaagctgct gggattcgta ttccagtcgt
     1861 tcaacctgct gccgttcaag aacgccgcgg aaaacgtcgc gctgccgctc tattaccagg
     1921 gcgtcggccg caaagagcgc aactatcgcg ccgagcagtt tctcgacatg gtgggtttgt
     1981 ccgatcgcaa atcgttcatg ccgaaccagt tgtcaggcgg gcagaagcaa cgcgtggcca
     2041 ttgcccgtgc gctggtggcc aatccgaaag taatcctcgc cgatgagccc acgggccagc
     2101 tcgacagcaa gtcgacggag gagatcgtcg cgctgctcaa ggacatccat cggcgcggca
     2161 acaccatgat catcgtgacc cacgaacagg acatcgccga ccagaccgaa aggcagatca
     2221 tcctgaaaga cggcgtggcc acctcgacgc agctcgcaga gcggcaggcc gagacaaact
     2281 agtcatgctg gacggcctgc aggaaatatt cttcacgttg cggcagaaca agcttcgcac
     2341 gctgctgacc gcgttcggcg tgttctgggg tattttcatg ctggtgttgc tgttaggcgc
     2401 cggacgcggg atgcagaacg gcgtatacga agacttcggc tcggatgtgc tcgatttcat
     2461 catcgtgtgg accggcacga cgaccacggc gtaccgcggc atgccacctg gtcgggccat
     2521 tcgcctcaac ctggaggaca tcgaagcggt ccgtcgacag atctccggca tcaggttgtt
     2581 gtccgctgag cgcttcgtcc cggacggcgg caccatcaac tac
//
LOCUS       DATDEU010000706         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1595100, whole genome shotgun sequence.
ACCESSION   DATDEU010000706 DATDEU010000000
VERSION     DATDEU010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595100"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VIV63_16895"
     CDS             complement(<1..1470)
                     /locus_tag="VIV63_16895"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6126333.1"
                     /translation="MFVTFTALADDPSMDEVVVTAELRDRNLRDLAASATVLEAHTLE
                     TAGVQHFQDVLGLVPNLNWSGGTSRPRFFQLRGIGELSQWQGAPNPSVGFLIDGIDFS
                     GVGMPATLNDVDRIEVLRGPQGTAYGANALAGLIAVNTRAPNREHEMNADVSFGDFGT
                     RGMSGVLGGPVGDGEAAWRLVAGTYRSDGFRRNSYLGRDDTNGYQESNARFKYRAQPL
                     ESLRVDVTAMWADLDNGYDAFSIDNSRTTLSDKPGQDTQIARALAVRLDFDGAETFDV
                     VSRTAFGTSRSVYSFDGDWGNDESWGANSPYDYFQRFDRERATLSTDLRLVSRTSVES
                     GDDFAWLAGAYVLRTDEDVQQHDIYRDLVYGDGERLFSSDYRATNLAGFGELEWRLGA
                     STTLALGARAERRSADYEDTDAAAFSPDETMYGGSLSLRRALGEQSTAYVKLARGYKA
                     GGFNIGDAVPDDRRSFDAEVLHSLELGFRAASADSSLAGD"
     regulatory      complement(1497..1612)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1673..>2559
                     /locus_tag="VIV63_16900"
     CDS             1673..>2559
                     /locus_tag="VIV63_16900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEY6126334.1"
                     /translation="MSRRIRIFQVDAFTADRFTGNPAGVVLDADSLSDAEMAAIAREL
                     NNADTAFVLKPDSADHDLRVRFFTPRTEASFVGHATVATHFVLSREGAPALHALGAGG
                     RVRQRQKSGLVDVEVRGAGAARRIAVRQPAPPLGRQLNDRERLAVLDALALSTGDIDT
                     RCPLRIVGGAGTRLIIGVRSTEQLKQLKPDYSRLTTLSAQLGAAGFFVFTLSHGFTGC
                     LTESRMFCPALGIPEDPVSGNAHGLLGAYLAEQGLLPHSGPMAQFTGAQGHHVHRAGR
                     VDVELEFSESKLDGVWIVG"
BASE COUNT          433 a          860 c          825 g          441 t
ORIGIN      
        1 atcgccggcg agcgatgaat cggcgctggc cgcgcggaac cccagctcga ggctgtgcag
       61 cacttcggcg tcgaagctgc ggcggtcgtc cggcacggca tcgccgatgt tgaagccacc
      121 cgccttgtaa ccacgcgcca gcttgacgta ggcggtgctc tgttcgccga gagcgcggcg
      181 aagactcagg gacccgccat acatggtttc gtcgggcgaa aacgcggcag cgtccgtgtc
      241 ttcatagtcg gcgctgcggc gctcggcacg cgctcccagc gccagggtcg tcgacgcacc
      301 gagccgccac tcgagctcgc cgaatccggc gaggttcgtc gcgcgatagt cgctcgagaa
      361 tagccgctcg ccatcgccgt acacgaggtc gcgatagata tcgtgctgct gaacgtcttc
      421 atccgtgcgc aacacataag caccggcgag ccacgcgaaa tcgtcgccgc tttccacgga
      481 ggttcgcgag acgagccgca ggtcggtgct gagagtcgcg cgctcgcgat cgaatcgctg
      541 gaagtaatcg tacggcgagt tcgctcccca gctttcgtcg ttaccccagt cgccatcgaa
      601 ggaatacacg ctgcgcgaag tgccgaacgc cgtgcggctc acgacgtcga aggtttccgc
      661 gccatcgaaa tcgagacgca ccgcgagcgc tcgtgcgatc tgcgtgtcct gcccgggctt
      721 gtcggacagc gtggtgcggg agttgtcgat cgagaacgcg tcgtagccgt tgtcgagatc
      781 ggcccacatg gcggtcacgt cgacgcgcag gctttccagc ggctgcgcgc gatacttgaa
      841 gcgcgcattg ctctcctggt aaccgttggt gtcgtcgcgt cccaggtatg aattgcgccg
      901 gaagccgtcg cttcgatagg tgccggcgac gagccgccac gcggcttcgc cgtcacccac
      961 cggaccgcct aacacaccgc tcatgccgcg tgtcccgaag tcgccgaagc tgacatcggc
     1021 attcatttca tgctcgcggt tcggcgcccg cgtgttgacg gcgatcagac ccgcgagtgc
     1081 attggcgccg taagccgtgc cctgcgggcc gcgcaggact tcgatgcgat ccacgtcgtt
     1141 gagcgtggcg ggcattccga cgccggagaa gtcgatgcca tcgatgagga aacccaccga
     1201 gggattcggc gcgccctgcc attgagacaa ctcgccgatt ccgcgcaact ggaagaagcg
     1261 cgggcgcgac gtgcctcccg accagttgag attgggcacg agtcccagca cgtcctggaa
     1321 atgctgcacc ccggcggttt cgagcgtatg cgcctcgagc accgtggcgc tggccgcgag
     1381 gtcgcggaga ttgcggtcgc gcagctccgc cgtgaccacg acttcgtcca tcgacggatc
     1441 gtcggccagg gccgtaaagg tgacaaacaa gaatggaatc gcgcacgcgg cgcgcaatga
     1501 agagtgcatc cctatccctc cgccggtact agccggatca ggttctacgg gttcgcggcc
     1561 catccgcgtc tcaggccggt gcattgcgca ctggccaccc cgaggcgagc cggatagtaa
     1621 cggaatttga gagcttgagt gacacgggtt tgcgggtaga attcgcgccc cgatgagccg
     1681 ccgaatccgc attttccagg tagacgcatt cactgcggat cgcttcaccg gcaatcccgc
     1741 gggagtggtg ctggacgccg attcactcag tgacgcggaa atggccgcca tcgcgcgcga
     1801 actcaacaac gcggacaccg cgttcgtgct caaacccgat tccgccgacc acgacctgcg
     1861 cgtgcgtttc ttcacgcctc gcactgaagc gagcttcgtc ggccacgcca cggtcgccac
     1921 gcatttcgtg ctgtcgcgag agggcgcgcc ggcgttgcat gcgctcgggg ccggcggccg
     1981 cgtgcgccag cgtcagaaat cgggattggt cgacgtcgaa gtgcgtggcg cgggcgccgc
     2041 acgccgcatc gcggtgcgtc aaccggcgcc gccgctcggc cgccagctca acgatcgcga
     2101 acgcctcgcc gtgctcgatg cactggcgtt gtccaccggc gacatcgaca cgcgttgccc
     2161 gctgcgcatc gtcggcggcg cgggcacccg gctcatcatc ggcgtgcgca gcaccgagca
     2221 gctcaaacag ctcaaacccg actactcgcg cctgactacc ctgtccgcgc agcttggcgc
     2281 cgccggcttc ttcgtcttca cgctgtcgca cggtttcacg ggttgcctca ccgagtcgcg
     2341 catgttctgc ccggcgctcg gcatccccga agacccggtg agcggcaatg cgcatggttt
     2401 gttaggcgcg tacctcgccg agcagggtct gctgccgcat tcaggtccga tggcgcagtt
     2461 caccggagca cagggccacc acgtgcatcg cgccggacgc gtcgatgtcg aactcgagtt
     2521 cagcgaatcc aaactcgatg gcgtgtggat cgtgggcca
//
LOCUS       DATDEU010000707         5104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_866706, whole genome shotgun sequence.
ACCESSION   DATDEU010000707 DATDEU010000000
VERSION     DATDEU010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866706"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1400)
                     /locus_tag="VIV63_16905"
     CDS             complement(180..1400)
                     /locus_tag="VIV63_16905"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6126335.1"
                     /translation="MHANLRHLHTFREVARLGSVSAAARSVHISQPAVTQAIAGLERF
                     FGSPLLLRRSNGVSLTSAGEVCLARIDRSMSQLRDAVIVTTRGSGAERANVERLVRSR
                     QLDALGAVVEYGNFSIAARARHVSQPSIHRSARELERVLGVPLFEKTSFGITPTREAA
                     ELARRTRLAFAEIEQARAEVHALSGGESGRTVVSALPLARSYLLPAALLEFTQKHPRH
                     GVAIIDGTYEHLLAGLRAGESDILIGALRDPAPAADVRQEHLFDDPLAIIARAGHPLA
                     RMRRISSAALRKYQWIAPRDGSPLRVHFDALLASSGLELPSPPLECNSMAAARAFLLE
                     SDRIMLLSAHQIHYELLAGLLVALPHPAGNVVRPIGLTLRHNWHPTSTQTTLLEILRQ
                     TARTAAAIHAKRRA"
     gene            1510..2193
                     /locus_tag="VIV63_16910"
     CDS             1510..2193
                     /locus_tag="VIV63_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEY6126336.1"
                     /translation="MSVVVRNVQRVERSVAESLAPFGVATIHEAQGRTGLMAPYMRPI
                     YPGAVIAGPAVTVSVPPGDNWMIHVAVEQCQAGDVLVVAPTSHSDCGYFGDLLATSLR
                     ARGVLGLVIEAGVRDVPTLKEMGFPVWSRYISAQGTIKATLGSVNIPLVCAGALVNPG
                     DLVVADDDGVCVVPRDTAPTVLASSRARELKEAGSRARLASGELGLDLYGMREALAAK
                     GLTYRDAGD"
     gene            2198..3280
                     /locus_tag="VIV63_16915"
     CDS             2198..3280
                     /locus_tag="VIV63_16915"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HEY6126337.1"
                     /translation="MLTGIPCTLMRGGTSKGLYFHAADLPPEPELRDRVLLAAMGSPD
                     VRQIDGVGGAHPLTSKVAVISRSTHESADVDYLFLQVVVDRAEVSDSQNCGNLLAGVG
                     PWAIENGLVPVTGASTPVRIHMVNTGSMAVAHVQTPEGAVEYEGEARIDGVPGTAAPV
                     TLEFLDVAGSSSGSLLPTGAVADRFEDIELTCIDNGMPVIIVRAEDLGVTGAESPAQL
                     EANAALVARVERIRLAMGPRMNLGDVTRKTVPKICLVGAPRKGGSIATATFIPHRVHE
                     AIGVLGAVSVATACVLPGSVAARVARLDPAARRIEVEHPSGFFTVDVEIESTEPIVVR
                     GAALLRTARKLLRGDVFVPGAVWSRA"
     gene            3277..4164
                     /locus_tag="VIV63_16920"
     CDS             3277..4164
                     /locus_tag="VIV63_16920"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEY6126338.1"
                     /translation="MTGLSVCLLGFGEVGQVLSQDLRSRVTQLVAWDLQFKDAASAPS
                     RAASRDDVLVARDVKQAVAHADLVISAVTAAQTGEAALSVAPYLKREAWFFDLNSTSP
                     AAKQQAAGVVDAAGGRFIEAAIMSPISPRRVASPMLLGGVHAAGFIPLARELGFSGVE
                     AFSGKLGAASAAKMCRSVVVKGMEALLLESLLTARQHSVDAAVLDSLQSMFPGGDWRE
                     TARYMISRALIHGRRRAEEMREAAHTVSQAGFSSPMSSACAEWEDWASHHSAAATHQD
                     IGAMLDALLTAKQGERAPC"
     gene            4158..>5104
                     /locus_tag="VIV63_16925"
     CDS             4158..>5104
                     /locus_tag="VIV63_16925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6126339.1"
                     /translation="MLIIDCHGHYTTAPDRHQHFRDAQLVRIKDSTLPAPVPQPVSDD
                     EIRESIETNQLRLQRERGADLTLFSPRASAMGHHVGDEAVSMAWTRACNDLVKRVVDL
                     YPENFAAVCQLPQSPGVPIAHSVDELTRCVRELGFVGCNLNPDPSGGHWNSPPLTDRH
                     WYPFYEKMVELDVPAMVHVSASCNPNFHATGAHYINADTTAFMQFIQGDLFRDFPTLR
                     FVIPHGGGAVPYHWGRYRGLADMLKRPALDGHVMRNVFFDTCVYHQPGIDLLVKVIDT
                     PNILFGSEMVGAVRGIDPTTGHYFDDTKRYIDALTIAAE"
BASE COUNT          804 a         1682 c         1738 g          880 t
ORIGIN      
        1 caactgcgaa cacttcgtcg agtggtgtct gcacgacgtg caccgcagtt ttcaggtgaa
       61 gaccgcgctc gaatttcccc ggtggttcgg cgagagaatc ctggcggcgc tgcatcgctg
      121 gctcgaccgc ggacgtgctc cgttgccaca gcggattcgc tgaagcggga gtgtccccat
      181 tatgcgcggc gcttcgcgtg gatcgcggcg gcggttcgcg cggtttggcg caggatttcc
      241 agcaaggtcg tctgcgtgct ggtcggatgc cagttgtgac gcagcgtgag gccgatcggc
      301 cgcaccacgt tgccagcggg gtggggcaat gccaccagca ggcccgccag cagttcatag
      361 tgaatctgat gcgccgacag cagcatgatg cggtcgctct cgagcagaaa cgcgcgggcc
      421 gccgccatcg aattgcattc gagcggtggg ctcggcagtt cgagcccgct ggacgcgagc
      481 agcgcatcga agtgcacgcg cagcggagaa ccgtctcgcg gcgcaatcca ttggtacttg
      541 cgtagcgcgg cggagctgat gcggcgcatt cgggccagtg ggtgcccggc gcgcgcgatg
      601 atggccagcg gatcgtcgaa caggtgctcc tggcggacgt cggccgccgg tgccgggtcg
      661 cgcagggcgc cgatcaggat gtcggattcg ccggcgcgca gccccgcgag caggtgttcg
      721 tacgtgccgt cgatgatcgc cacgccatgc cgcggatgtt tctgcgtgaa ctcgagcaac
      781 gcggccggca gtagatagct gcgcgccagc ggcagcgcac tcacgaccgt gcggccggat
      841 tcgccgccac tcaatgcgtg gacttcggca cgcgcctgct cgatctcggc gaaggccagg
      901 cgcgtgcggc gcgccagttc ggcggcttcc cgcgtcggcg tgatgccgaa gctggtcttc
      961 tcgaacaacg gcacaccgag aacgcgctcg agctcccgcg cggagcgatg gatgctgggc
     1021 tgggacacat ggcgggcgcg ggccgcgatg ctgaagttgc cgtattccac caccgcgccg
     1081 agcgcgtcga gctggcgcga gcgcaccagt ctttccacgt tggcgcgttc cgcgccgctg
     1141 ccgcgcgtgg taacgataac cgcgtcgcgc agctggctca tcgagcgatc gatgcgcgcg
     1201 aggcatacct cgcccgcgct ggtgagcgag acaccattgc ttcgccgcag cagaagcggc
     1261 gaaccgaaga aacgttcgag tccggcgatg gcctgcgtga ccgcgggctg cgagatgtgc
     1321 accgaacgcg cggccgcgct gacgctgccg agacgcgcga cttcgcgaaa cgtatggaga
     1381 tggcggaggt tggcgtgcat ggcgcatcct acacctatag cgttttctta tggccatgcc
     1441 ggttttctga ttggccgcga ggcaggtgcg ggcggagaat gccgcacgtc gacaacgggg
     1501 ggtggacgga tgtcggtggt agtacgaaac gtgcagcgcg tcgagcgcag tgtcgcggag
     1561 tcgctggcgc cttttggcgt ggccaccatc cacgaggcac agggccgcac cggcctcatg
     1621 gcgccctaca tgcgccccat ctacccgggc gcggtcattg caggtccagc cgtcacggtc
     1681 agcgttccgc ccggcgacaa ctggatgatt cacgttgccg tggagcaatg ccaggcgggc
     1741 gacgtgctcg tggtggcgcc cacgtcgcat tccgactgcg gctacttcgg tgacttgtta
     1801 gccacttcgc ttcgggcccg cggcgtgctc ggcctcgtca tcgaggccgg cgtccgcgac
     1861 gtcccgacgt tgaaggagat gggtttcccc gtgtggtcgc gatacatctc ggcgcagggc
     1921 acgatcaagg cgacgctggg cagcgtcaac attccgctgg tctgcgcggg tgcgctcgtg
     1981 aaccccggag atctggtggt cgcggacgac gacggtgtgt gcgtggtgcc gcgcgacacg
     2041 gcgcccaccg tgctcgccag ttcgcgcgcg cgcgagctca aggaggcggg gtcgcgcgca
     2101 cgtctcgcat cgggcgagct gggtctcgat ctctacggca tgcgcgaggc gctggccgcg
     2161 aagggactca cttaccgcga cgccggggac tgaacgcgtg ctcaccggaa ttccctgcac
     2221 cctcatgcgc ggcggaacgt cgaagggcct ctatttccat gccgccgatc tgccgcccga
     2281 acccgagctg cgcgaccgcg tgctgctcgc ggccatgggc tcaccggacg tgcgccagat
     2341 cgacggagtc ggcggcgcgc atccgctcac cagcaaggtg gcggtgatct cccgctcgac
     2401 gcacgaaagc gcggacgtcg attacctttt ccttcaggtg gtcgtggatc gcgccgaagt
     2461 gagcgacagc cagaattgcg gcaacctgct cgccggagtc ggtccctggg ccatcgagaa
     2521 cggactggtg ccggtcacgg gtgcttcgac gcccgtgcgc attcatatgg tgaacacggg
     2581 aagcatggcg gtggcgcacg tgcagacgcc cgagggtgcc gtcgaatacg aaggcgaggc
     2641 gcgcatcgat ggcgtgccag gcaccgccgc gcccgtgacg ctcgaattcc tcgatgtcgc
     2701 cggctcgagc agtggctcat tgctgcccac gggcgcggtc gcggaccgct tcgaggacat
     2761 cgagctcacc tgtatcgaca acggcatgcc cgtcatcatc gtgcgcgccg aagatctcgg
     2821 cgtcaccggc gcggagtcgc ccgcgcaact cgaagccaat gcggcgctcg tcgcccgggt
     2881 cgagcgcata cgcctggcaa tgggcccgcg catgaatctc ggcgatgtga cgcgcaagac
     2941 cgtgccgaag atctgcctgg tcggcgcgcc gcggaagggc ggttcgatcg ccacggccac
     3001 gttcatcccg catcgcgtgc acgaggccat cggcgtgttg ggcgccgtca gcgtggcgac
     3061 cgcctgcgtg cttccaggat ccgtggccgc ccgcgtggcg cggctcgatc ctgccgcgcg
     3121 acggatcgaa gtcgaacatc ccagcggatt tttcaccgtc gacgtggaga tcgaatcgac
     3181 cgagcccatc gtcgtgcgtg gcgcagcgct gctgcgcacg gcgcgcaagc ttctgcgcgg
     3241 cgatgtgttc gtcccgggcg ccgtctggag ccgcgcatga cggggctgtc ggtctgcctg
     3301 ctcggtttcg gcgaagtggg ccaggtactg agccaggatc tgcgctcgcg cgtcacccaa
     3361 ctggttgcct gggatcttca attcaaggat gccgccagcg ctccgagccg cgcggcatcg
     3421 cgcgacgacg tgctcgtcgc gcgcgatgtc aaacaagcgg tggcgcatgc ggatctggtg
     3481 atcagcgcgg tcaccgcggc gcagacaggc gaggccgcgc tgtcggtcgc tccgtatctc
     3541 aagcgcgaag cctggttctt cgatctgaac tcgacgtcgc cagccgccaa acaacaggcc
     3601 gcgggtgtcg tcgacgcggc gggcggccgc ttcatcgagg ccgccatcat gtcgccgatc
     3661 tcgccgcgac gggtggcatc acccatgttg ctcggtggcg tacacgcagc cggcttcatt
     3721 ccgctggcgc gcgagctcgg cttttccggc gtggaggctt tttccgggaa gttaggcgcc
     3781 gcgtccgccg ccaagatgtg ccgcagcgtc gtggtcaagg ggatggaggc gctgttgctc
     3841 gagtcgctgc tgacggcgcg gcaacacagc gtggacgccg cggtgctcga ctcgctgcag
     3901 tcgatgtttc ccggcggcga ctggcgggaa accgctcgtt acatgatttc ccgcgcgctc
     3961 atccatgggc ggcggcgcgc cgaggaaatg cgcgaggcag cgcacaccgt ttcacaggca
     4021 gggttctcgt cgcccatgag cagcgcctgt gccgaatggg aggattgggc atcacatcat
     4081 tccgcggccg ccacgcacca ggacatcggc gcgatgctcg atgcgttgct gaccgcgaaa
     4141 cagggtgagc gcgcgccatg ctgatcatcg actgccacgg gcactacacc acggcgcctg
     4201 atcggcacca gcacttccgc gacgctcaac tcgtgcgcat caaggactcc acgttgcctg
     4261 cgcccgtgcc gcagccggtc agcgacgatg aaatccgcga gtcgatcgag accaatcaac
     4321 ttcgtctgca gcgcgagcgc ggagccgacc tcacgctgtt ttcgccgcgc gcatcggcca
     4381 tgggccatca cgtgggcgac gaagccgtct ccatggcgtg gacgcgcgcg tgcaacgacc
     4441 tcgtgaaacg cgtggtcgat ctgtaccccg agaatttcgc ggccgtctgc cagctcccgc
     4501 aatctcctgg cgtgcccatc gcgcactccg tggatgaact cacgcgctgc gtgcgcgagc
     4561 tcggtttcgt gggatgcaat ctcaatccgg atccgtcggg cggccactgg aactcgccgc
     4621 cgctcaccga tcgccactgg tatccgttct atgaaaagat ggtcgagctc gatgtgccgg
     4681 ccatggtcca cgtctcggcg tcgtgcaacc ccaatttcca cgccactggc gcgcactaca
     4741 tcaatgccga taccacggcg ttcatgcagt tcatccaggg cgacctgttc cgcgatttcc
     4801 ccacgctgcg tttcgtcatc ccgcatggcg gtggcgcggt gccgtatcac tggggacgct
     4861 atcgcggtct cgccgacatg ctcaagcgtc cggcgctcga cggccacgtc atgcgcaatg
     4921 tctttttcga cacctgcgtc tatcaccagc cgggcatcga tctgctggtc aaggtgatcg
     4981 acacccccaa catcctgttc ggctcggaga tggtgggcgc cgtgcgcggg atcgacccga
     5041 ccacgggcca ttacttcgac gacaccaagc gttacatcga tgcgctgacg atcgccgccg
     5101 agga
//
LOCUS       DATDEU010000708         2173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1450758, whole genome shotgun sequence.
ACCESSION   DATDEU010000708 DATDEU010000000
VERSION     DATDEU010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450758"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VIV63_16930"
     CDS             <1..1214
                     /locus_tag="VIV63_16930"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY6126340.1"
                     /translation="KPGTARLAMLMVQEGTKTRTSPEIADRAESLGAPLAVGSTLDRS
                     FLNMNALSNRLGESLELYADVLLNPTFPEKELERLRGQTLATIQQEKAQPPAIINRVM
                     PKLIYGEGHAYSNPASGTGTAEAVSSLTSAELQAFYKRWVRPDNSLLLVVGDTTLASI
                     QPLLEQRFGQWRAPADAKPAKNLATVALATKPRVFLIDRPGAEQSQIVAATVGPTRAD
                     PDHIRFVALDTMLGGNFVSRLNMNLREDKHWAYGAGSRITDAIGQGMFRAGAGVQTDK
                     TAESIVEIRKEFRDIVGSRKPTAAELKFAQDSIAIALPGNNETSDEIANSYGEILTFG
                     LKDSYWNDFVGDLTALTPADVNKSAGRLVYPDALTWVIVGDLAKIEKGVRALNIGEVT
                     VLDTDGKVVAR"
     gene            complement(1215..1595)
                     /locus_tag="VIV63_16935"
     CDS             complement(1215..1595)
                     /locus_tag="VIV63_16935"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY6126341.1"
                     /translation="MRLLLDTQIFLWWLADSRKLAKANRERIEAADEVYVSAASIVEC
                     EQKISSGLLEADAHELARGIRASGFTELPVRAKPAADAASLKPHPGVDFFDRLLIAQA
                     MHEPLRFLTANAALKQYSELVEVA"
     gene            complement(1621..1851)
                     /locus_tag="VIV63_16940"
     CDS             complement(1621..1851)
                     /locus_tag="VIV63_16940"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY6126342.1"
                     /translation="MKTAKINIHEAKTHLSRLIEDVAGGSEILIAKGGRAMARLVPLR
                     RDDTPRKPGMLKGRMRISDDFERPWTDSSPPA"
     gene            1900..>2173
                     /gene="ftsB"
                     /locus_tag="VIV63_16945"
     CDS             1900..>2173
                     /gene="ftsB"
                     /locus_tag="VIV63_16945"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEY6126343.1"
                     /translation="MKYLAGALLTVVLLLQYRLWLSGDGVRELARLSEAVERQKSDNE
                     EAAERNKQLIAEVADLKAGMAAIEERARSELGMIGRNETFYQVVPVR"
BASE COUNT          404 a          686 c          714 g          369 t
ORIGIN      
        1 cgaagccggg tacggcgcgc ctcgcgatgc tcatggtcca ggaaggcacc aagacccgaa
       61 cctcaccgga gatcgcggac cgcgcggaat cgctcggcgc gccgctcgcc gtgggatcga
      121 cgctcgatcg gtcgttcctc aacatgaatg cgctcagcaa ccggctcggt gagtcgctcg
      181 agctgtacgc cgacgtgttg ttgaacccca cgttcccgga gaaggaactg gaacggctgc
      241 gtggtcagac attggcgacg atccagcagg agaaggcgca gccgccggcc atcatcaatc
      301 gtgtgatgcc gaagctcatc tacggtgaag gccatgcgta ctccaacccg gcctcgggca
      361 cgggcacggc ggaagccgtt tccagtctca ccagcgcgga gctgcaggct ttctacaagc
      421 gctgggtgcg gcccgacaac tcattgttgt tagtggtggg cgacacgacg ctcgccagca
      481 tccagccgct gctcgagcag cggtttggac agtggcgcgc gcctgcggac gcgaagccgg
      541 cgaagaacct ggccaccgtg gcgctcgcga ccaagccgcg cgtgttcctc atcgaccgtc
      601 ccggggccga gcaatcgcaa atcgtggcgg ccaccgtggg accgacgcgc gccgatccgg
      661 atcacatccg cttcgtcgcg ctcgacacca tgctcggcgg caatttcgtc tcgcgcctca
      721 acatgaatct gcgcgaagac aaacattggg cctatggcgc gggctcgcgc atcacggacg
      781 cgatcggcca gggcatgttc cgcgcgggcg ccggcgtgca gaccgacaag accgccgagt
      841 ccatcgtcga gatccgcaag gagtttcgcg acatcgtcgg ttcgcggaag cccaccgcgg
      901 ccgaactcaa gttcgcgcag gacagcatcg cgatcgcgct gcccggcaac aacgagacct
      961 ccgacgagat cgccaattca tacggcgaga tcctgacgtt cggcctgaag gacagttact
     1021 ggaacgactt cgtcggggat ctcaccgcgc tcacgccagc cgacgtcaac aagtccgcgg
     1081 gccggctcgt gtatccggat gcgctcacct gggtgatcgt gggcgatctc gcaaagatcg
     1141 agaagggcgt gcgcgccctc aatatcggcg aggtgacagt gctcgatacc gacgggaagg
     1201 tggtggcgcg gtaatcacgc cacctcgacg agttccgaat attgcttcag cgccgcattt
     1261 gcggtgagga agcgcagcgg ctcgtgcatc gcctgggcga tcaaaaggcg atcgaaaaag
     1321 tccacgccag gatgcggctt cagtgaagcc gcatccgcgg cgggcttcgc gcggaccggc
     1381 aactcggtga acccgctggc gcgaatgccg cgcgcgagtt cgtgcgcgtc ggcctcgagc
     1441 aggcccgacg aaatcttctg ttcgcattcc acgatggacg ccgcgctgac gtagacctcg
     1501 tccgcggctt cgatgcgctc gcggttggcc ttggccagct tgcgagagtc cgcgagccac
     1561 cagagaaaaa tctgcgtatc gagcagcagg cgcatggatg gcggagtagg ggagggcggc
     1621 tcaggcgggc ggcgaactgt cggtccaggg tcgctcgaag tcatcggaga tgcgcatcct
     1681 ccccttgagc attcccggtt tgcgcggcgt gtcgtcgcgc cgcagcggta ccagcctggc
     1741 catggcccgg cctcccttgg cgatcaggat ttcactgcca ccggccacgt cttcgatgag
     1801 ccgcgacaga tgcgtcttcg cttcatggat gtttatcttc gcggtcttca cagtgaactt
     1861 agtttagttt agtctagtgg ctatggggaa ggagttttcg tgaagtacct cgcaggcgcg
     1921 ttgctgacag tcgtattgct gctgcagtac cggctctggc tgtccggcga cggcgtgcgc
     1981 gagctcgcgc ggctgtcgga agccgtggag cgccagaagt cggataacga ggaagcggcg
     2041 gagcgcaaca agcagctcat cgccgaagtg gccgacctca aggccggcat ggcggctatt
     2101 gaagaacgcg cccgaagcga actcggcatg atcggccgca acgaaacctt ctaccaggtc
     2161 gtgccggtcc ggc
//
LOCUS       DATDEU010000709         4590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_282515, whole genome shotgun sequence.
ACCESSION   DATDEU010000709 DATDEU010000000
VERSION     DATDEU010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282515"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..731
                     /locus_tag="VIV63_16950"
     CDS             156..731
                     /locus_tag="VIV63_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126344.1"
                     /translation="MNMRWLTALLLVIPAWAQAGVGDWYTTVYGARISTQPGWEDIII
                     DAPGTEFVDSYLATIAVSRQYAERKDGALAIEAEGQVVYHFHDQTLWEFNAVPILLRW
                     KRFPWSDRFNSSAAFGLGLSYATEIPPVEVMLEGESHQLLVYWVMELTAGPRAAPWEI
                     SLRLHHRSVAYGLMGDEGGMNAVGLGLRYRF"
     gene            complement(719..1414)
                     /locus_tag="VIV63_16955"
     CDS             complement(719..1414)
                     /locus_tag="VIV63_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY6126345.1"
                     /translation="MRSTLAATILLVGIASLAACSRGEPPAPTSSAPPAAAAEQPPSA
                     AEPAPAALTPEQTARLVRPHSPVLGAAKARVTVVEVLDPACEACRAFAPVVEQLLFLY
                     PDDVRVVVRFADFHPVSLQAIRLLEAAHQQGKFHVLLSALYDRQQEWASHSAPDPARA
                     WKIAGDVGIDIARARKDALAAKVDDMLRLEAEDLAELKVERTPTFFVNGKPLPSFGDK
                     QLLDLVASEVQNR"
     gene            complement(1411..2901)
                     /locus_tag="VIV63_16960"
     CDS             complement(1411..2901)
                     /locus_tag="VIV63_16960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6126346.1"
                     /translation="MSSLIPEMQDLDPDGQRLPVKLDSATNGEFAPIPLSRSLHHANH
                     LAHEWAGELSRKLGQSRRSFLTTACGAASTLLAFNAAHARAGREGGFFEIANEATVDE
                     QLAASQLGKREFIFDVQGHFVNPTGAWTKRLPAGEKPLQFPKTEQCELARQPGERSYL
                     NCIGPDEFIKDVFLDSDTDLMVLSFVPSTREDEPLTIEEANATRDIVEKMEGSKRLLL
                     HGRVNPNQKGDVEDMDRLAKFGVVAFKTYTQWGPSGAGFWMTDDVGIAFVEKARKLGV
                     RNICIHKGFDFGPKSYEHSTCRDIGPIAKRFPDMNFLIYHSGFVSTKPEGAYDPQRTD
                     GIDALITSLATSGIKRNGNVYAELGSTWRFLSMRDPDSAAHAMGKLFKYVGEDNMLWG
                     TDSIWYGSPQDQIQAFRTFQISEALRDRYAYPKMTPQLRAKVFGLNATRPYRLAADEL
                     RLFTAGDKVAQSKAEYSERPNPSYATYGPRTRREFLNLRAWHGGEP"
     gene            3011..3445
                     /locus_tag="VIV63_16965"
     CDS             3011..3445
                     /locus_tag="VIV63_16965"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6126347.1"
                     /translation="METQELYRGRLTDHIQLVVKDLAASKRFYTAVFKVIEIPLAGEG
                     DDYFWADELFISTKESAAAQGSLTGRMHIAFQGKSREMVEQFYKAALAAGGRDNGAPG
                     ERSYHPGYFGAFVIDPDGNNIEVVFHGPATKSADSVKITFES"
     gene            3449..3619
                     /locus_tag="VIV63_16970"
     CDS             3449..3619
                     /locus_tag="VIV63_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126348.1"
                     /translation="MTPFVISMIVVGAILVGTLFGLLRGRRDPMGSPEVLERAKQRNR
                     ELEAQEKLEDDR"
     gene            complement(3621..>4590)
                     /locus_tag="VIV63_16975"
     CDS             complement(3621..>4590)
                     /locus_tag="VIV63_16975"
                     /inference="COORDINATES: protein motif:HMM:NF005460.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY6126349.1"
                     /translation="YSGLGINPHYGTPRNAFDRPAARIPGGSSSGAAVSGADGMAVAA
                     IGTDSGGSVRIPAALSGLVGFKPTARRVPLDGVLPLSLTLDSAGPIANSVADCALLDL
                     VLSSSPIIELPQVNLRGTRLAIPQSVAMEDLSTPVARAFASAVAKLSAAGAMVVEIPL
                     SEFARAAEINPRGAISSAEAFWWHRPWIKDGADQYDPRVIARIRPGEAISAAGYIELL
                     QQRRRFVTDVEAAVRGFDALLMPTIADTAPTIAEVVASDESYFRFNGRMLRNAALVNL
                     FDGCALSLPCHPSGEAPVGLMIAGLAGQDRHVLALGLAAEQVLRKP"
BASE COUNT          842 a         1426 c         1494 g          828 t
ORIGIN      
        1 ggctcacgca ccagcgagtg ccgacccagg ccttctcctc gaggacctcg ggaagatcga
       61 ggcacaccag acgaagcctt gagagagcgt ctcggaaaac gccttgagca tgaattccca
      121 cagccccgaa tcataagcgc aggtacgctt cgcccatgaa catgcgatgg ctgaccgcac
      181 tccttcttgt gataccggcg tgggcccagg ccggtgtggg cgactggtac accacggtgt
      241 atggcgcacg gatttccacg cagcccggct gggaagacat catcatcgat gcgcccggca
      301 ccgaattcgt ggactcctac ctggccacca tcgccgtctc acgccagtac gcggaacgaa
      361 aagatggcgc gctcgcgatc gaggcggaag gccaggtcgt ctaccatttt catgaccaga
      421 cgctgtggga gttcaacgcg gtccccatcc tgctgcgatg gaaacgcttt ccctggagcg
      481 atcgattcaa ctcatcggcg gctttcggtc tcgggctgtc gtacgccacc gagatcccgc
      541 ccgtcgaggt gatgctcgaa ggcgaaagtc accagctgct ggtgtactgg gtcatggaac
      601 tgaccgcggg cccgcgcgcg gccccgtggg aaatctcgct gagactgcat caccgctcgg
      661 tcgcatatgg actcatgggt gatgaaggcg gcatgaacgc cgtgggtctc ggactgcgct
      721 accggttctg aacttcgctg gccaccaggt cgagcagttg cttgtcgccg aagcttggca
      781 gtggtttgcc gttcacgaaa aaagtgggcg tgcgttcgac cttgagctcc gcgagatctt
      841 cagcctcgag acgcagcatg tcgtcgacct tcgcggcgag cgcatctttg cgtgctcttg
      901 cgatgtcgat tccgacgtct cccgcgatct tccatgcgcg tgcgggatcc ggcgcggagt
      961 gtgatgccca ttcctgctgc cggtcgtaca aggcactcag cagcacgtga aattttccct
     1021 gctggtgcgc ggcttccagc aggcggatgg cctgcagcga gacgggatgg aagtccgcga
     1081 agcgaacgac cacgcggaca tcgtcgggat agaggaacag caactgctcg accacgggag
     1141 cgaaggcgcg gcaggcttca caggcggggt cgagcacctc gaccaccgtc acgcgcgcct
     1201 tcgcggcacc gagcaccgga gaatgcggac gcaccagcct tgcggtctgc tcgggagtca
     1261 gcgcagccgg tgcgggttcg gccgcggacg gaggttgttc ggccgcggca gcgggtggcg
     1321 cggatgacgt tggcgccgga ggttctccgc gcgaacacgc ggccaaagag gcgatgccga
     1381 ccagcaggat agtcgcggct aatgtggatc tcatggctct ccgccgtgcc aggcccgcag
     1441 gttgaggaat tcccgtcgcg tgcgcggacc gtaagtcgca tacgaaggat tgggtcgctc
     1501 ggaatactcg gctttggatt gcgccacctt gtcaccggcc gtgaacaggc gaagttcgtc
     1561 ggccgcgagc cggtaaggcc gcgtggcgtt caatccaaaa accttggcgc gcaattgcgg
     1621 cgtcatcttc gggtaagcat accggtcccg caacgcttcg gagatctgaa acgtgcggaa
     1681 cgcctggatc tgatcctgcg gcgagccgta ccagatggaa tccgtgcccc agagcatgtt
     1741 gtcctcgccc acgtacttga atagtttgcc catcgcatgc gccgcggaat ccggatctcg
     1801 catcgagagg aaccgccatg tcgaaccgag ctcggcatag acgttgccat tgcgtttgat
     1861 gccgctcgtg gcgagcgacg tgatcaatgc gtcgatgccg tcggtgcgtt gcggatcgta
     1921 tgcaccctcg ggtttggtcg agacgaaccc ggagtggtag atcaggaaat tcatgtccgg
     1981 aaatcgcttc gcgatcggtc cgatatcgcg gcaggtcgag tgttcgtagc tcttcgggcc
     2041 gaagtcgaaa cccttgtgga tgcagatgtt gcgcacgccg agttttcgcg ccttctcgac
     2101 gaaggcgatg ccgacatcgt ccgtcatcca gaagcccgcg ccgctcggac cccactgcgt
     2161 atacgtcttg aacgcgacga cgccgaactt cgcgaggcga tccatgtcct ccacgtcgcc
     2221 tttctgattg ggattcacgc ggccgtgcag cagcaggcgc ttcgagcctt ccatcttctc
     2281 gacgatgtca cgtgtcgcat tggcttcttc gatggtcagc ggctcgtctt cacgcgtcga
     2341 cggaacgaag ctcagcacca tcagatcggt atcggagtcg aggaacacgt ccttgatgaa
     2401 ctcgtcgggg ccgatgcagt tgagataact gcgctcgcca ggttgtctgg cgagttcgca
     2461 ctgctccgtc ttcggaaact gcagcggctt ctcgccggcc ggcaggcgct tcgtccatgc
     2521 gcccgtggga ttcacgaagt gaccctgcac atcgaatatg aattcccgtt tcccgagctg
     2581 cgaagccgcg agttgttcgt cgaccgtggc ctcgttcgcg atctcgaaga atccgccttc
     2641 gcggccggca cgcgcgtgcg cggcattgaa cgccagcaac gtggaagccg cgccgcaggc
     2701 ggtcgtcagg aacgagcggc gcgattgccc gagtttccgt gacagctcac cggcccactc
     2761 gtgagccagg tggttcgcat ggtgcaggga acgggacagc ggaatcggcg cgaattcgcc
     2821 attcgtggcg ctgtcgagtt tgaccggcag ccgctgacca tccgggtcga gatcctgcat
     2881 ttccggaatg aggctggaca tggcgatcct ccgggaacct ggggtggcgc agtgtgccac
     2941 tcgcgcggcc cgccgtgcag tcagcatcgg ctgattccgc gggcacggag gtggtgttag
     3001 gttcgggggc atggaaaccc aggaactgta tcgcgggcga ttgaccgatc acatccaact
     3061 ggtggtcaag gatctcgcgg cgagcaaacg cttctatacc gccgtcttca aggtcatcga
     3121 gatcccgctg gccggagaag gtgacgatta cttctgggcc gacgagctgt tcatctcgac
     3181 gaaggaaagc gccgcggcac aagggtcgtt gacgggccgc atgcatatcg cgtttcaggg
     3241 caagagccgc gagatggtcg agcagttcta caaggcggcg ctggcggcgg gcggacggga
     3301 caacggcgcg ccgggcgaac gttcgtatca tcccggctat ttcggcgcgt tcgtcatcga
     3361 cccggacggc aacaacatcg aagtggtgtt ccacggcccg gcgacgaagt cggcggattc
     3421 cgtgaagatc acgttcgaga gttaggccat gactcctttt gtaatctcca tgatcgtggt
     3481 cggtgccatc ctggtgggca ccctgttcgg tctgttgcgc ggccgccgtg atcccatggg
     3541 atcacccgaa gtgctggagc gcgcgaagca gcgcaatcgc gaactcgaag cgcaggagaa
     3601 gctcgaggac gatcgttagc tcacggcttc cgcagcacct gctcggcggc cagccccagc
     3661 gccagcacgt gtcgatcctg acccgccagg ccagcaatca tgagacccac gggcgcttcg
     3721 ccggacgggt gacacggcaa gctcaacgcg cacccgtcga atagattgac gagcgccgcg
     3781 ttgcgcaaca tccggccgtt gaaacggaag taactctcgt cgctcgccac cacttcggca
     3841 atcgtcggcg ccgtatcggc aatcgtcggc atcagcaacg cgtcgaagcc gcgcaccgcc
     3901 gcttcgacgt ccgtcacgaa acgccgccgc tgctgcagga gctcgatgta accggcagcg
     3961 ctgatcgcct cgccgggacg aatgcgcgcg atgactcgcg gatcgtattg atccgcgccg
     4021 tccttgatcc aggggcgatg ccaccagaac gcttccgcgc tgctgatggc gccgcgcgga
     4081 ttgatctcgg ccgcgcgcgc gaattcgctg agcgggatct cgacgaccat ggcgcccgcc
     4141 gcggacaatt ttgcgaccgc cgacgcgaag gcccgcgcta ccggcgtgga taggtcttcc
     4201 atcgccacgg actgcgggat tgccaggcgc gtgccgcgca ggttcacttg cggtagttcg
     4261 atgatgggcg atgacgacag aaccaggtcg agcagcgcgc agtcggcaac ggaattggcg
     4321 atgggccccg ccgaatcgag cgtcagcgac aacggcaaca caccgtcgag cggcacgcgg
     4381 cgcgccgtgg gtttgaaacc cacgaggcca ctgagcgccg cggggatgcg caccgagcca
     4441 cccgaatccg tgccgatggc cgcgacggcc atgccgtcgg cgccggaaac ggccgcgccg
     4501 gatgacgacc ctccgggaat tcgggccgcg ggacgatcga acgcattacg cggtgtgccg
     4561 tagtgagggt tgatgccgag gccggagtac
//
LOCUS       DATDEU010000710         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1887636, whole genome shotgun sequence.
ACCESSION   DATDEU010000710 DATDEU010000000
VERSION     DATDEU010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887636"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VIV63_16980"
     CDS             complement(<1..183)
                     /locus_tag="VIV63_16980"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY6126350.1"
                     /translation="MRIALVAGETSGDTLGAALVEGLRRRFPHAEFAGVAGPKMRAAG
                     CVAWHDIESLSVMGLTE"
     gene            complement(198..971)
                     /gene="lpxA"
                     /locus_tag="VIV63_16985"
     CDS             complement(198..971)
                     /gene="lpxA"
                     /locus_tag="VIV63_16985"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY6126351.1"
                     /translation="MAIDSRAIVSPKAELAEGVTVGPYTVIEDGVQIGKGTWIGPHAV
                     IKGPTTIGEDNKIFQFASIGDAPQDLKYKGEPTRLTIGDRNVFREFTTMNRGTVGGGG
                     LTVIGSDNLFMAFTHVAHDCRVGNKIVMANYATLAGHVQLGDWVIMGGYSGTHQFTKV
                     GAHAFIGNNAAVTRDVPPYVMAVGTPAVPHSINSEGLKRRGFTPEQIRNLKNAYRVLY
                     RSDMKLADAVAELRMRAETQPELRIFVDFIGESTRSLVR"
     gene            complement(982..1461)
                     /gene="fabZ"
                     /locus_tag="VIV63_16990"
     CDS             complement(982..1461)
                     /gene="fabZ"
                     /locus_tag="VIV63_16990"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY6126352.1"
                     /translation="MTESISLDIMGVRRLLPHRYPFLLVDRVLECVKGERIKAIKNVT
                     TNEPFFPGHFPHRPVMPGVMILEALAQAAGILAFITADVVPDESTRFFFVGIDKARFR
                     KPVEPGDQLILTATFERSFKGIWKFDTIAYVGDKEVASAEMMVAPETNKTSAKTPPA"
     gene            complement(1466..>2126)
                     /gene="lpxD"
                     /locus_tag="VIV63_16995"
     CDS             complement(1466..>2126)
                     /gene="lpxD"
                     /locus_tag="VIV63_16995"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY6126353.1"
                     /translation="IGPGCVIERGAVIGDDSRLVARVFVGPHVVFGNRCIVQAGAVVG
                     GDGFGYAPEKGAWIKVPQLGGVTIGDDVEIGANTTIDRGALDDTVIEEGVKLDNLIMI
                     AHNCRIGAHSAIAAQSAIAGSSVLGKRCIMGGRAGLTGHITLCDDVVVLGTSFISHSI
                     TKPGVYSSALPSEEAGAWRRIVARIKRLDSMAKRLRNVEKHVGLGSASASLDDRDSSE
                     D"
BASE COUNT          368 a          679 c          692 g          387 t
ORIGIN      
        1 ttcggtcagg cccatgaccg atagcgactc gatgtcgtgc caggccacgc agcccgcggc
       61 gcgcatcttc gggcccgcga cgccggcgaa ctcggcatgc gggaagcgtc gcctgagccc
      121 ctccacgagg gcggcgccga gcgtgtcgcc agaggtttcg ccggccacga gtgcgatacg
      181 cacagcgcgt cctctcgtta acgaacgagg ctgcgggtcg actcgccgat gaagtcgacg
      241 aagatcctga gttcgggctg ggtttcagcg cgcatcctga gctcagcgac ggcatcggcg
      301 agtttcatgt cggagcggta caacacgcgg taggcattct tgaggttgcg gatctgttcc
      361 ggcgtgaagc cgcgacgctt gagcccttcg gaattgatcg aatgcggcac ggcaggcgtg
      421 cccaccgcca tcacgtaagg aggtacgtcg cgggtcaccg cggcattgtt gccgatgaac
      481 gcgtgcgcac ccaccttggt gaactggtgc gtgcccgagt agccgcccat gatgacccag
      541 tctcccagtt gcacgtgtcc ggccagggtc gcgtagttag ccatgacgat cttgttgccg
      601 acgcggcagt cgtgcgccac gtgagtaaag gccatgaaca ggttgtcgct gccgatgacc
      661 gtgaggccgc cgccgcccac cgtgccgcga ttcatggtgg tgaactcgcg aaacacgttg
      721 cggtcgccga tcgtaaggcg cgtgggctca cccttgtact tgagatcctg cggcgcatcg
      781 ccaatggacg cgaactggaa aatcttgttg tcttcaccga tggtggtcgg acccttgatg
      841 accgcatggg gcccgatcca ggtgcccttg ccgatctgca ccccgtcttc gatgacggtg
      901 tacggcccca cggtcacgcc ttccgcgagt tcggccttcg gagaaactat ggctcttgag
      961 tcgattgcca tgggctcgaa ctcacgccgg cggcgtcttg gcggaagtct tgttggtctc
     1021 gggcgcgacc atcatctcgg cggacgcgac ttccttgtcg cccacgtagg cgatggtgtc
     1081 gaacttccag attcccttga agctgcgctc gaaggtggcc gtgaggatga gctggtcgcc
     1141 gggctcgacc ggtttgcgga aacgcgcctt gtcgatgccc acgaagaaga agcgcgtcga
     1201 ctcgtcggga accacatcgg cggtgatgaa ggccaggatg ccggcggcct gcgccagcgc
     1261 ctcgaggatc atgacgcccg gcatcacggg ccggtgcggg aaatggccgg ggaagaacgg
     1321 ctcgttggtg gtgacgttct tgatcgcctt gatgcgctca cccttgacgc attccagcac
     1381 gcggtccacg agcaggaagg ggtagcgatg cggcagcagc cggcgtacgc ccatgatgtc
     1441 gagtgaaatc gactcggtca ttcagttagt cctcggaaga atctcggtcg tccaacgaag
     1501 cagaggctga acccaggccc acgtgtttct ccacgttgcg cagccgcttg gccatcgaat
     1561 cgagccgctt gatgcgcgcc acgatacgcc gccaggcccc ggcctcctcg ctcggcaggg
     1621 ccgaagaata cacgcctggc ttggtgatgg aatgcgatat gaaagaggtg cccagcacca
     1681 ccacgtcgtc gcacagcgtg atgtggccgg tgaggccggc ccggccgccc atgatgcacc
     1741 gtttgcccag cacggaactg ccggcgatgg cgctctgcgc cgcgatcgcc gagtgcgcgc
     1801 cgatgcggca gttgtgcgca atcatgatga gattgtcgag cttgacgcct tcctcgatta
     1861 ccgtgtcgtc gagggcaccg cggtcgatgg tggtgttcgc gccgatctcc acgtcgtcac
     1921 cgatggtcac gccgcccagc tgcggcacct tgatccaggc gcctttctcg ggtgcgtacc
     1981 cgaagccgtc accgccaacc accgcgccgg cctggacgat gcaacggttg ccgaatacca
     2041 cgtgaggccc gacgaacacc cgcgccacca gccgcgagtc atcgccgatg acagcgccgc
     2101 gctcgatcac gcatccggga ccgatg
//
LOCUS       DATDEU010000711         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1596565, whole genome shotgun sequence.
ACCESSION   DATDEU010000711 DATDEU010000000
VERSION     DATDEU010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596565"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VIV63_17000"
     CDS             <1..920
                     /locus_tag="VIV63_17000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY6126354.1"
                     /translation="GAGPEHSGVVLLDDLGVVAPAPPQVAPLMRRIDEGELASALPRR
                     ARDSHKGTHGHVLVIGGGAGMPGALRLAGEAALRVGAGRVTVAGVPDNLVAVTATRPE
                     LIYLPVSSVTAIDEAIRGCDVLAIGPGLGTGDWARRLWASACSVTGITAIVDADALNL
                     LAANPGKLPPGWIITPHPGEAARLLGVDTGAVQADRLGAARELHARYGAVTVLKGAGT
                     LVCAATDAGGVEMTICDRGNPGMATAGMGDVLTGVIAGLRSQCGASALAARIGVLVHA
                     LAGDSAAQGGQRGLVASDVIAELRGWVNP"
     gene            917..1375
                     /gene="tsaE"
                     /locus_tag="VIV63_17005"
     CDS             917..1375
                     /gene="tsaE"
                     /locus_tag="VIV63_17005"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY6126355.1"
                     /translation="MTPEEPRAWVTRSGEETEALGARLLGTPPTRDAPCRVVFLRGEL
                     GSGKSTFARGALRALGVTGPIKSPSYTLLETYELPAITAVHLDLYRLLDPEELEHLGL
                     ADYHRPGFLWLVEWPERGDGRMPAADLELQFSIGPDGHRIERIETFKPLK"
     gene            1405..>2585
                     /locus_tag="VIV63_17010"
     CDS             1405..>2585
                     /locus_tag="VIV63_17010"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY6126356.1"
                     /translation="MTGKDFFRTGVWLALLASVAGVSGNAVAAEIREVRVAATESGSR
                     VVLDLSGPVKHKAFLLEGPNRVVLDVSKSSLNSKLPGAEGVITAMRSGELPHNGLRLV
                     FEVMGPVTFETSVAAPSGDAGHRLILDIAGSSKTMANVRPVVATPAVPAAPVAIRPAH
                     APLDSGRDIVIAIDAGHGGVDPGASGKRGTREKNVVLEIAKALAARINAEPGMKAVLT
                     RDGDYFISLKERTLRARRAKADLFVSIHADAIANPDVSGSSVYVLSDRGASSEAARWL
                     AERENAADLKGGIKLDDKDAVLANVLLDLSQTASISASMVAAENILKSLDQIGEVRKP
                     RVQQAGFVVLKSPDIPSMLVETAFISNREDERKLSLPAHRAKLANAIFAGIEQYFQGN
                     AP"
BASE COUNT          405 a          815 c          920 g          445 t
ORIGIN      
        1 tgggcgcggg tcccgagcat tcaggcgtgg tgctgctcga cgatctcggc gtggtggcgc
       61 cggccccgcc gcaggtcgcg ccgctcatgc gccgcatcga cgaaggagag ctcgcctcgg
      121 cgctgccgcg ccgcgcacgc gattcgcaca agggcaccca cggacacgta ctggtcatcg
      181 gcggcggcgc ggggatgccg ggcgcgctgc gcttggcggg cgaggcggct ctgcgcgtgg
      241 gcgccggccg ggtcacggtc gccggggtgc ccgacaatct cgttgcggtc accgcgacgc
      301 gccccgagct catctatcta ccggtttcga gtgtgaccgc gatcgatgaa gccattcgcg
      361 gctgcgacgt gctggcgatc ggtcccggcc tcggaaccgg cgactgggcg cggcggttgt
      421 gggcgtccgc gtgttcggtg acgggcatta cggccatcgt cgatgccgat gcgctgaacc
      481 tgctggccgc gaacccgggg aaattgccgc ccggctggat catcacgcct caccccggcg
      541 aggccgctcg attgttaggc gtggacaccg gggcagtgca ggcggaccgc ctgggtgcgg
      601 cccgcgagct tcatgcgcga tacggcgccg tgacggtgct caagggtgcg gggacgctgg
      661 tgtgtgccgc gacggatgcg ggtggcgtcg agatgaccat ctgcgaccgc ggcaatcccg
      721 gcatggccac ggccggcatg ggcgacgtgc tcaccggcgt catcgccgga ttgcgatcac
      781 aatgtggcgc gagcgcattg gcggcacgca tcggcgtgct ggtgcacgcg ctggcgggag
      841 attcggcggc gcagggcggg caacgcggtc tcgtcgccag tgacgtcatc gccgaactgc
      901 gcggctgggt gaatccttga cgccggagga gccgcgagcg tgggtcacgc gcagcggcga
      961 ggaaaccgaa gcgctggggg cccgcctgct cggaacgcct ccgacgcgtg acgctccgtg
     1021 ccgcgtggtc ttcctgcgag gtgagctcgg ttcgggcaag tcgactttcg cgcgcggcgc
     1081 gctgcgtgcg ctcggcgtga ccggtccgat caagagtccg agctacacgc tgctcgaaac
     1141 ctacgaactg ccggcgatca ccgcggtcca tctggatctg taccggttgc tggatcccga
     1201 agaactggag cacctggggc tcgccgacta tcaccggccg gggttcctgt ggctcgtcga
     1261 atggccggag cgcggtgacg gccggatgcc cgccgcggac ctcgaattgc agttttcgat
     1321 cggtccggat ggtcatcgaa tcgagaggat tgaaactttt aagccactta agtaaattgc
     1381 gcctcacatc aggctcggaa ctccatgacg gggaaggact tttttaggac tggggtttgg
     1441 ctggcgctgc tcgcatcggt tgcgggagtt tccgggaacg ccgtcgccgc ggaaatccgc
     1501 gaggtgcgcg tcgccgccac cgaatccggt tcgcgcgtgg tgctcgatct ctcgggtccc
     1561 gtcaaacaca aagccttcct gctcgagggt ccgaaccgcg tggtgctgga cgtctcgaag
     1621 tcatcgctga attcgaaatt gcccggggcc gagggcgtca tcactgccat gcgttcgggc
     1681 gaattgccgc acaacggatt gcgactggtg ttcgaagtca tggggccggt taccttcgag
     1741 acgtcagtgg cggcgccgtc cggtgatgcc ggtcaccgcc tgatcctgga tatcgcgggc
     1801 tcatcgaaga ccatggccaa cgtgagaccc gtggtcgcga cgccggccgt tcctgccgcg
     1861 ccggtggcca tccgtcctgc gcatgcgccc ctcgattcgg gtcgcgacat cgtgatcgcg
     1921 atcgacgcag gtcacggagg cgtcgatccg ggcgcatccg gcaagcgcgg cacgcgagag
     1981 aaaaacgtgg tgctggaaat cgcgaaggcg ctggctgcgc gcatcaacgc ggagccaggc
     2041 atgaaggcgg tgctgacgcg tgacggggac tacttcattt cgttgaagga acgcacgctg
     2101 cgcgcacggc gcgcgaaggc ggacctgttc gtgtccatcc atgccgacgc catcgccaat
     2161 ccggacgtgt cgggttcgtc ggtgtacgtg ctgtcggatc gcggcgcttc cagcgaggcc
     2221 gcccgctggc tggccgagcg tgaaaacgcg gccgacttga agggcggcat caagctcgac
     2281 gacaaggacg cggtgctcgc gaacgtgttg ctcgacctgt cgcagaccgc gagcatctcc
     2341 gcgagcatgg tggcggccga aaacatcctg aagtcgctcg accagatcgg tgaagtccgc
     2401 aagccgcgcg ttcagcaggc cggtttcgtc gtgctcaagt cgcccgatat tccgtcgatg
     2461 ctcgtcgaga ccgcgttcat ctccaatcgc gaagacgagc gcaagctgtc gttgccggcg
     2521 caccgcgcca agctcgcgaa tgcgatcttt gcgggaatag agcagtactt tcagggcaat
     2581 gcgcc
//
LOCUS       DATDEU010000712         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1451713, whole genome shotgun sequence.
ACCESSION   DATDEU010000712 DATDEU010000000
VERSION     DATDEU010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451713"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VIV63_17015"
     CDS             complement(<1..634)
                     /locus_tag="VIV63_17015"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126357.1"
                     /translation="MKFDKPIVIGARASVLAQVQARLVGDALLARHPGLKVEYSFLTS
                     PGDREFDTPLPELLRRGGFTGDLGVALRANSIDIAVHLWKDLPFATHPLTHIAATLPR
                     ADTRDLLLVKRSWIGGRDRSELRVLSSSARRRTNLSGFLQWALPIKPPRGTFVLARGD
                     CEARIAQLVDGDSAGLVVAKANIDRLLESTQPEFAAIRERVRAALDACEIM"
     gene            complement(772..1566)
                     /locus_tag="VIV63_17020"
     CDS             complement(772..1566)
                     /locus_tag="VIV63_17020"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY6126358.1"
                     /translation="MIESVARELPKFSAAPVSLVGAPRLTLSPKEFAADADASKRIND
                     WLASVDAATRVRWALANLPGPFALSSSFGAQAAVSLHLVTQADPTIPVLVIDTGYLFP
                     ETYQFIKQLEQQLELNIQTFSSPQTVDDFETQYGKLWEQGREGLDKYLELRKVEPMRR
                     GLATLGTATWFAGLRRSQAASRAALNPLEVRDGRFKVHPIFDWSDRDVYLYLKAHELP
                     YHPLWDKGYVSIGDWHSTKALHEVESADELRFSGLKRECGIHGLEG"
     gene            complement(1563..>2196)
                     /locus_tag="VIV63_17025"
     CDS             complement(1563..>2196)
                     /locus_tag="VIV63_17025"
                     /inference="COORDINATES: protein motif:HMM:NF015425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126359.1"
                     /translation="HLTLRIEAGRVGDTPGKPFLTGIEKIAAIHQGDFRLTANQNLII
                     ANVPASECMNIDRLVADYGLGKALFTSPLRRDALACVSLPTCSLAMAEAERYLPDFVD
                     RVDELLNKHGLKGSPLTVRITGCPNGCARPYLAEIGLIGRAPGRYTLRLGADAVGSRL
                     NVVYRDNIDENAMITALDELIGRYAVERHADERFGDFLWRANVLKQESRA"
BASE COUNT          362 a          677 c          796 g          361 t
ORIGIN      
        1 ccatgatctc gcaggcgtcg agcgcggcgc gcacacgctc gcgaatggcg gcgaattccg
       61 gctgcgtgga ttcgagcagc cggtcgatgt tggccttggc caccacgagg ccggcggaat
      121 cgccgtccac gagctgcgcg atgcgcgcct cgcaatcgcc gcgcgcgagc acgaacgtgc
      181 cgcgcggtgg tttgatgggc agcgcccact gcaggaaacc ggagaggttg gtgcggcgtc
      241 gcgccgacga cgacaacacg cgcaactcgc tgcggtctcg gccaccgatc cagcttcgct
      301 tcaccagcag caggtcgcgc gtgtcggcgc gcggcagggt cgcggcgatg tgcgtcagcg
      361 ggtgggtggc gaacggcagg tccttccaga ggtgcacggc gatatcgatg gagttggcgc
      421 gcagtgcaac gcccagatcg cccgtgaagc caccgcgccg caggagctcg ggcagcggcg
      481 tgtcgaattc gcgatcgccg ggggacgtga ggaaggaata ttcgaccttc agacccggat
      541 gacgcgcaag caaagcatcg ccgacgaggc gcgcctgcac ctgcgccagc acactggcgc
      601 gcgcgcctat cacgatgggt ttgtcgaact tcatatcctg catgggaaag gaagcaatcc
      661 ctgtgccacg gaatgcgcgg tgctggtcgg cttggcgtcg gcgggccgcg cactatgggt
      721 gcggccggtc atcggtgatg cgcggctacg gtgcagccgc ggggcaaacc cctaaccttc
      781 cagcccgtgg attccgcact cacgtttgag tccggagaac cgcaattcat ccgccgactc
      841 gacttcatgc agcgccttgg tggagtgcca gtcgccgatg gacacgtatc ccttgtccca
      901 cagcgggtgg tacggcagtt catgcgcctt gagatacagg tacacgtcgc ggtcggacca
      961 gtcgaagatg gggtggacct tgaagcggcc gtcgcgcact tcgagcggat tcagcgcggc
     1021 gcggctggcg gcctggctgc ggcgcagacc ggcgaaccac gtggcggtgc cgagggtcgc
     1081 gagcccgcgg cgcatgggtt cgaccttgcg cagctcgaga tatttgtcga gcccttcgcg
     1141 tccctgctcc cagagcttgc cgtactgcgt ctcgaagtcg tcgacggtct gcgggctcga
     1201 gaaggtctgg atgttgagct cgagctgctg ctcgagctgc ttgatgaact ggtaggtctc
     1261 gggaaataga tagccggtgt cgatgaccag caccgggatg gtggggtcgg cctgcgtgac
     1321 gaggtgcagc gacaccgcgg cctgcgcccc gaagctcgac gacagcgcga acgggcccgg
     1381 gagattggcc agcgcccagc gaacacgcgt ggcggcgtcc accgaagcaa gccagtcgtt
     1441 gatgcgcttg ctggcgtccg cgtcggcggc aaactctttg ggcgagagcg tcagacgcgg
     1501 cgcgccgacc agcgacaccg gggcggcgct gaatttcggc agttcgcggg cgacggactc
     1561 gatcacgcgc gcgactcctg cttgagcacg ttggcgcgcc agaggaagtc gccgaagcgt
     1621 tcgtcggcgt gccgttcgac ggcataccgg ccgatgagct catcgagcgc ggtgatcatg
     1681 gcgttctcgt cgatgttgtc gcgatacacg acgttcagtc gcgagcccac ggcgtcggcg
     1741 ccgaggcgca gcgtgtagcg gccgggagcg cggccgatca ggccgatctc ggcgagatag
     1801 ggacgcgcgc agccgttggg gcatccggtg atgcgcaccg tcagaggcga acctttgagc
     1861 ccgtgcttgt tgagtagttc gtcgacacga tcgacgaagt ccggcaggta acgctccgct
     1921 tcggccatgg ccagcgagca ggtgggcagc gagacgcagg ccagcgcatc gcggcgcagc
     1981 ggcgacgtga acagcgcctt gcccaatccg tagtcggcca cgaggcggtc gatgttcatg
     2041 cattcgctgg cgggaacgtt cgcgatgatg aggttctggt tggcggtgag ccggaaatcg
     2101 ccctggtgga tggcggcgat cttctcgatg ccggtgagga acggcttgcc gggcgtgtcg
     2161 ccgacgcggc ccgcctcgat gcgcagcgtg agatgc
//
LOCUS       DATDEU010000713         4827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_868140, whole genome shotgun sequence.
ACCESSION   DATDEU010000713 DATDEU010000000
VERSION     DATDEU010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868140"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VIV63_17030"
     CDS             complement(<1..1321)
                     /locus_tag="VIV63_17030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6126360.1"
                     /translation="MPRTMVAQTPAEIRPASQLTDGSDARLRALTEHALEIITVQSAD
                     GTFSYANEAVARYLGYSVPELLGRNAAEFLHPDDVEAMRERFRSLLSLARRGSQPELN
                     RFEYRFRHRDGTWIWLESVAVNALDNPTVLGIIAHSRDINRRKANERILSLHHARYRT
                     VADLSEGAVHEYLMNAEGDYELEWTLGAERVYGCTDEEYRRRGWQSFLEGEGWLAQSL
                     ERTQHYLQGETVEFTAQIRRPDGELRWLEVKNRPIADPVTGKFTRLVGVAIDITERKH
                     AADALRESEFRYRTVAELTSGFVYEATVDEHGEMQVVWASPGFTPFFGGSFEELNRLG
                     WRQAFFPGDYASAIQRRQKLRRGERTEIEIAVKTLRNETRWIHLANQPVLNADGTVTR
                     YIGVVHDITDRKANEEVLRSQALAIEVMHEGVVLSDSGGIIRMTNPAF"
     gene            1917..4781
                     /locus_tag="VIV63_17035"
     CDS             1917..4781
                     /locus_tag="VIV63_17035"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6126361.1"
                     /translation="MDEVVVTGSRITSPNLQSISPITAIGAEELNLAGKARIEDVLNQ
                     LPQAFAAQGSNISNASDGTASVDLRGLGSQRTLVLVNGKRLMPGDPDGGSAADLNQIP
                     LSLVKRVDVLTGGASSVYGADAVAGVVNFVMDNEFEGVRIDANYSFYNHSNDSERTQA
                     INEARGFALPESTVNGGYSKDYSIAVGIGGAEGRGHATFYATYREVDAVLQADYDFSS
                     CTLNLAQAGTDYTCGGSGTASPAQFLIVNPTTGALSGGRVTNPDGTMRAFTAADQYNF
                     GPVNFFQRPDERFTGGVFANFKLSDAADVYGEFMFMDDRSVAQIAPSGAFLGAATFAL
                     NCDNPLWSAAQRQEFCGAFGLGAGDFAQVLIGRRNTEGGGRQDDIGHESFRTVVGLRG
                     EINDWLSYDSYYMHGETKRNITYLNDFSTRRTALALNAADDGAGGVACLVNTDADPAN
                     DDANCVPWNIWQAGGVDPASLAYLQTPGFQRATAKTNILHADMTVDLSGSVKLPSTET
                     GLVFNVGAEYRDENTEFQVDEAFRTGDLSGQGGATLPVLGRFDVSELFMEARLPLIEG
                     KTGAQTLSIEAGYRFSDYSTGFSTDTYKAGLDWAPIDMLRLRGSYQRAVRAPNVGELF
                     SSQSVALDGTEDPCAGATPEATLAECELTGMTAAQYGNVPENPAGQYNGLLGGNPELD
                     AETSDTVSFGFVLRPEIGDLSIAVDYFDIKVEDTISSTAGGNADTYITNCIDTGQADF
                     CDLINRDEFGSLWLSATNAYIVDTSLNLGTLRTRGVDLQASYTLNLGEHRLGFNMVGT
                     KLMEYSNAPLPGYPSYECKGFYGGTCGIPAPEWRHSFRTNWRTPWNGLDVAATWRYYG
                     DAKSERLSTNPQLSGPVNANGISNGVPAYSYIDLTAAMTFAEKFTFRVGANNLFDKSP
                     PIIPSGGVVDCPSGPCNGNTWAQVYDAMGRQVFATVTVDF"
BASE COUNT          875 a         1506 c         1502 g          944 t
ORIGIN      
        1 cgaacgccgg gttcgtcatg cggatgatgc cgccgctgtc ggacagcacg acgccttcgt
       61 gcatcacttc gatggcgagc gcctggctgc gcagtacttc ttcattggcc ttgcggtcgg
      121 tgatgtcgtg caccacgccg atgtatcgcg tcacggttcc gtctgcgttc agcacgggtt
      181 gattggccag gtggatccag cgcgtttcgt tgcgcagggt ctttaccgcg atctcgatct
      241 cggttcgttc gccacgtcgg agcttctgcc gtcgttgaat ggcgctggcg tagtcgccgg
      301 ggaaaaacgc ctgccgccag ccgaggcgat tcaactcttc gaatgagcca ccgaagaagg
      361 gcgtgaaacc aggacttgcc cagacgacct gcatctcgcc gtgttcatcg accgtggcct
      421 cgtagacgaa acccgacgtc agctccgcga ccgtgcggta gcgaaactcg ctttcgcgca
      481 gcgcatcggc ggcgtgcttg cgctcggtga tgtcgatggc gacaccgacg aggcgcgtga
      541 acttcccggt gaccggatcg gcaatcggcc gattcttcac ttcgagccag cggagctcgc
      601 cgtcgggccg gcggatctgc gccgtgaatt cgacggtttc gccctggaga tagtgctggg
      661 tgcgttcgag gctttgcgcg agccatcctt cgccttcgag aaaactctgc cagccacggc
      721 ggcggtattc ctcgtcggta cagccgtaca cccgttcggc gcccagcgtc cattcgagtt
      781 cgtaatcgcc ctcggcgttc atgaggtact cgtgcaccgc gccttcggaa agatccgcta
      841 cggtccgata acgcgcgtga tgcagcgaca ggatccgctc gttcgccttg cggcgattga
      901 tgtcgcgcga gtgggcgatg atgccgagga ccgtggggtt gtcgagcgcg ttgacggcca
      961 cactctcgag ccagatccag gtgccatcgc gatgacgaaa gcgatattcg aaacgattga
     1021 gttcgggctg cgatccgcgc ctggcaaggg agaggaggct gcgaaagcgc tcacgcatcg
     1081 cctccacatc gtccggatgc aggaactcgg cggcattgcg gcctaacaac tcgggcaccg
     1141 agtaaccgag gtagcgggcc accgcttcgt tggcgtaact gaacgtgcca tccgcgcttt
     1201 gcacggtgat gatttcgagc gcgtgttcgg tcagcgcacg cagccgggcg tcggacccgt
     1261 cggtgagttg agaggcggga cggatttccg ctggtgtttg cgcgaccatg gtgcgcggca
     1321 attctatcga cagactcgca ttcgaaatcc gctgcgcgag cggaagcccg ccaggccgcc
     1381 cgcatcgccg cgcgttccac gtacgggacg tcgaccgctg ccgcccgtcc caacgtcaga
     1441 aagcggtgct acttttcgag tcatctgtgg cctgtgtaca acgttttggg gccctgtctt
     1501 agggcaaaaa tcataaatca ttgatatcta aaggcatcga tttccattcg gggctccaaa
     1561 cggctcgtcg aatctactgt tgtttgttgc gtagacgctg tagtaggctt cgccgcactg
     1621 caggggtcgg agtcgataca agagcgtaag caagatacgc gggctacata gggttctcca
     1681 agaaacctgg ccgcgtcttt tctttcgcgt tttgtctcga actcaggtct ctgttgccca
     1741 gccgtcgatg gaggttgggc gtaccaaatt cataaaccta taagggatca gtcatggcta
     1801 aacacctatc agtgcggcag gccgttcgac ttgctctcct cgcaagtgcg gcgccggcgg
     1861 tgcttctttc gcccgcggcg tttgcgcaag agcaggatca ggacgcaagt gattcgatgg
     1921 atgaagtcgt cgttaccggt tcgcgcatca cttcgccgaa ccttcagagc atcagcccga
     1981 tcactgcgat cggagccgaa gaactgaacc tcgcgggcaa ggcgcgcatc gaagacgttc
     2041 tcaaccagct cccgcaggca ttcgctgcgc agggctcgaa tatctccaac gcttctgacg
     2101 gcacggcgtc ggtcgacctg cgtggcttgg gctcgcagcg caccctcgtc ctcgtcaacg
     2161 gcaagcgcct gatgccgggc gacccggacg gcggcagcgc agccgacctc aaccagattc
     2221 ccttgtcact cgtgaagcga gtcgacgtgc tcaccggcgg tgcttcgtcg gtgtacggcg
     2281 ccgacgccgt cgcgggcgtc gtgaacttcg tcatggacaa cgagttcgag ggcgttcgca
     2341 tcgatgcgaa ctacagcttc tacaaccaca gcaacgacag cgagcgcacg caggccatca
     2401 acgaggctcg tggtttcgcg ttgccggaaa gcaccgttaa cggtggctac agcaaggatt
     2461 actcgatcgc cgtcggcatc ggtggtgccg aaggtcgcgg tcatgcgacc ttctatgcga
     2521 cctatcgtga agtcgacgcg gtgctccagg ctgattacga tttcagctcc tgcacgttga
     2581 acctcgctca agccggcacc gactacacct gcggcggttc gggcacggca agtccggcgc
     2641 agttcctgat cgtcaacccg acgacgggcg ctctctcggg cggtcgtgtg accaaccctg
     2701 acggcacgat gcgcgccttc acggcggccg accagtacaa cttcggtccg gtgaacttct
     2761 tccagcgccc tgatgaacgc ttcacgggtg gcgtgttcgc caacttcaag ttgagcgatg
     2821 cggccgacgt gtacggcgaa ttcatgttca tggacgaccg ttcagtggcc cagatcgcgc
     2881 cctccggcgc cttcctcggt gctgccacgt tcgcgctgaa ctgcgacaac ccgttgtggt
     2941 cggccgcgca gcggcaggag ttctgcggcg cgttcggact cggtgcgggt gatttcgccc
     3001 aggtgctcat cggtcgccgc aacacggaag gcggcggtcg tcaggacgac atcggccacg
     3061 aatcgttccg cacggtcgtc ggtctgcgcg gcgagatcaa cgattggctg agctatgaca
     3121 gctactacat gcacggcgag acgaagcgca acatcacgta cctcaacgac ttctcgaccc
     3181 gtcgtacggc gcttgcgctg aacgcggccg atgatggcgc gggcggcgtg gcttgcttgg
     3241 tcaacaccga tgccgatccg gccaacgacg acgcgaattg cgtgccgtgg aacatctggc
     3301 aggcaggggg cgtcgacccg gcgtcacttg cgtacttgca gacccctggc ttccagcgcg
     3361 cgacggcgaa gaccaacatc ctgcatgccg acatgacggt cgatctgtcc ggttcggtca
     3421 agctgccgag cacggaaacc ggtctcgtgt tcaacgtcgg cgccgaatac cgcgacgaga
     3481 acaccgagtt ccaggtcgac gaagctttcc gcactggcga tctctccggc cagggtggtg
     3541 caacgctgcc ggtgctgggt cgtttcgacg tgtccgagtt gttcatggaa gcgcgtctcc
     3601 cgctcatcga gggcaagacg ggcgctcaga cgctgtccat cgaagcgggc tatcgtttct
     3661 cggattacag cacgggcttc agcaccgaca cgtacaaggc cggcctcgac tgggcgccga
     3721 tcgacatgct gcgcttgcgc ggcagctacc agcgcgcggt gcgtgcacca aacgtcggcg
     3781 agttgttctc gtcacagtcg gtggctctcg acggaacgga agatccatgc gcgggtgcaa
     3841 cgcctgaggc gactctcgcc gagtgcgagc tcacgggcat gacggccgct caatacggaa
     3901 acgttcccga gaatcccgcc ggccagtaca acggcttgct gggtggcaat ccggaactcg
     3961 atgccgagac gtcggacacc gtgtccttcg gcttcgttct gcgtcctgaa atcggtgacc
     4021 tgtcgatcgc ggtcgactac ttcgacatca aggtcgagga cacgatcagc agcaccgcgg
     4081 gcggcaacgc cgatacgtac atcaccaact gtatcgacac cggccaggcc gacttctgcg
     4141 atctgatcaa tcgcgatgag ttcggttcgc tgtggctgtc ggccacgaac gcctacatcg
     4201 tcgacaccag cctgaacctg ggaacgctgc gtacccgcgg tgtggacctg caggccagct
     4261 acacgctgaa cctcggtgag catcggctcg ggttcaacat ggtgggcacg aagctgatgg
     4321 agtacagcaa cgctccgttg cctggctacc cgtcatacga gtgcaagggc ttctacggcg
     4381 gcacttgcgg aattccggcg ccggaatggc gtcattcgtt ccgcaccaac tggcgtacgc
     4441 cctggaacgg tctcgacgtg gcggccacgt ggcgctacta cggcgatgcc aagtccgagc
     4501 gtctgagcac caacccgcag ctgtccggtc cagtcaacgc caatggcatc tccaacggcg
     4561 ttccggcgta cagctacatc gacctcaccg cggccatgac cttcgccgag aagttcacgt
     4621 tccgcgtggg tgcgaacaac ctgttcgaca agtcgccacc gatcattccg tccggcggcg
     4681 tggtcgactg cccgtcgggt ccttgcaacg gcaacacctg ggcgcaggtt tacgatgcaa
     4741 tgggtcgcca ggtcttcgcg acggtgacgg tcgacttctg atcgaaagat cggttgcttg
     4801 atatgaacgg cgggcttcgg cccgccg
//
LOCUS       DATDEU010000714         4030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_868154, whole genome shotgun sequence.
ACCESSION   DATDEU010000714 DATDEU010000000
VERSION     DATDEU010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868154"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VIV63_17040"
     CDS             complement(<1..220)
                     /locus_tag="VIV63_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126362.1"
                     /translation="MATGAIRAICFDLDNTLWEVEPVLNRAERILADWLERRYPLIPQ
                     RFSPAAVLEMRAALLAEQPHQAHDLSFLR"
     gene            256..912
                     /locus_tag="VIV63_17045"
     CDS             256..912
                     /locus_tag="VIV63_17045"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6126363.1"
                     /translation="MLLTMGNKNYSSWSLRPWLLMKHLDLAFTERVIPLDTPEFARDV
                     VATSPTRRVPVLKHGSLLVWDSLAICEYACDLAGRGWPVERDARAVARSACAEMHAGF
                     SILRSQWPMNARATGRRTAPNPERSTEIARIESLWAECRTRFGANGPWLFGEFSVADA
                     MYAPVVLRLRTYGATVREATTAYMATVLADAHMHDWLQASAAESWIIEASEIGRQNTP
                     "
     gene            932..2983
                     /locus_tag="VIV63_17050"
     CDS             932..2983
                     /locus_tag="VIV63_17050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY6126364.1"
                     /translation="MKSWLRIATALACGICLEAAAADVASRTFTPTDLNKLARISDPQ
                     VSPDGHYVVYVQRETDLDTNRGRTDLWLLDLTSAKPKPRRLTQHSSNDTNPRWEVDGT
                     SIYFLSSRTGSQQVWRLPLTGGEAVQITDYPLDVATFRFSEGGGRIALSMEVFPDCAD
                     LKCSRDRLDGLSRKKETGRVFDSLFIRHWDTWNGGTRSNLFVAPMNTDGRAGTPLNVS
                     KALDADVPSKPDGGDEEYTFSPDGSRVVFSARVAGREEAWSTNFDLYEVPSDGSAAPR
                     NLTANNPAWDTQPVFLRNGDLAWLAMSRPGFEADRFRIMIRSDGEVSEVAPDWDRSVQ
                     HLDVAHDGRTLLATASDLGQTPLFRVDVDSGRVTPVSGKGYVGSFATTRRGTVVLWHD
                     LASPPDLYLLPNSGERRRLTNANSEILSARTLGAFEQFSFPGWNNETVYGYIVNPPGL
                     DKDRKYPVAFIVHGGPQVSFQNHWNWRWNAQTFAARGYATVFIDFHGSPGYGQAFTDS
                     ISQHWGDRPFEDLKKGFAAAVQKFPWLDGQRACSLGASYGGYMQNWIAGNWPDGFRCI
                     VNHDGIFDTRSMYYTTEELWFTEWENGGPYYQAPEIHERFNPAHHVKNWRTPMLVIHG
                     EQDFRVPDTQGIATFTALQRLGVESRLLYFPDENHWVLKPNNSIQWYGTVLDWLDAHT
                     K"
     gene            complement(2984..3448)
                     /locus_tag="VIV63_17055"
     CDS             complement(2984..3448)
                     /locus_tag="VIV63_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126365.1"
                     /translation="MNTAQRLAGPLALVAVLCGCTTPQQRDMQTIADFQRNTSGQYES
                     DSGAKLYIVPVRSRMVTDESMYIERHDANGIFGRLLDLKISPDGKKVTQAALAFTQEG
                     QWRNLRENPELFTALLPKDVRPAGTCDIQPTEDLNSVIYSCGGSPAELFRRL"
     gene            complement(3459..>4030)
                     /locus_tag="VIV63_17060"
     CDS             complement(3459..>4030)
                     /locus_tag="VIV63_17060"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126366.1"
                     /translation="TEDASLGISQASIVTDKAQLKQRIQFVHDQVQTDALVEEYIEGR
                     ELYVGVMGNDRLTRLPVWEMKFGSLAENSAGIATRRVKWDKKYQKKHGIDTYEAKELS
                     PAIVARLDKLSRRVYRSLGLSGCARIDYRMNAEGQIYVIEANPNPNLAADEDFAQSAL
                     AAGVSYPELLEKLLTLGAGYQAEWRAYYG"
BASE COUNT          719 a         1326 c         1276 g          709 t
ORIGIN      
        1 gccgcagaaa cgacaggtcg tgcgcctggt gcggttgttc ggcgaggagc gcggcgcgca
       61 tctcgagcac cgcggccggc gaaaaccgtt gcggtatgag cgggtatcgg cgttcgagcc
      121 aatcggcgag aatgcgctcc gcgcgattca gcaccggctc gacttcccag agcgtgttat
      181 cgagatcgaa gcagatcgcg cgaatcgcgc ctgttgccat ggcgtgcatg ttaccggggg
      241 accctcttgg acgaaatgct gctcaccatg ggcaacaaga actattcatc atggtcgctg
      301 cgcccgtggc tgctgatgaa acacctggac ctcgcgttca ccgagcgcgt gatcccgctc
      361 gacacacccg aattcgcgcg cgatgtcgtt gccaccagcc ctactcgccg cgtacccgtg
      421 ctcaagcacg gctcgctgct ggtgtgggac tcgctcgcca tctgcgaata cgcatgcgat
      481 ctcgccggcc gcggctggcc ggtcgagcgc gatgcacgcg ccgtagcgcg ctccgcgtgc
      541 gccgaaatgc acgctggatt ctcgatcctg cgttcacaat ggcccatgaa tgcgcgcgcc
      601 accggtcgca ggaccgcccc gaatccggaa cgctcgaccg agatcgcccg catcgaatcg
      661 ctctgggccg aatgccgcac gcgattcggc gcgaacggtc catggttgtt cggcgaattc
      721 agcgtcgccg acgccatgta cgcgcccgtc gtgctgcgat tgcgaaccta tggggccacg
      781 gtgcgcgagg ccaccacggc ctacatggcg accgtgctcg ccgacgcgca catgcacgac
      841 tggctgcagg cgtcggccgc ggaaagctgg atcatcgagg cgtccgaaat cggccgccag
      901 aacacgcctt aactaactac cggagatcgt catgaagtcc tggctgcgca ttgcaaccgc
      961 gttggcgtgt ggaatctgcc tggaggcggc cgccgccgac gtcgcgtcgc gcaccttcac
     1021 gcccaccgac ctcaacaagc tcgcgcggat cagcgatccg caggtgtcgc ccgacggtca
     1081 ctacgtggtg tacgtgcagc gcgagaccga cctcgatacg aatcggggcc ggaccgactt
     1141 gtggctgctg gacctgacga gcgccaagcc gaagccgcgc cgcctgaccc agcactcgtc
     1201 caatgacacg aatccccgct gggaagtgga cggcaccagc atctacttcc tgtcgtcgcg
     1261 caccggttcg cagcaggtct ggcgcctgcc gctgaccggt ggtgaagccg tgcagatcac
     1321 cgactacccg ctcgacgtgg cgacgttccg cttctctgaa ggcggcggcc gcatcgcgct
     1381 cagcatggaa gtattcccgg attgcgccga cctcaaatgc tcgcgcgacc ggctcgacgg
     1441 cctgagcaga aagaaggaaa cagggcgtgt tttcgacagc ctgttcatcc gccattggga
     1501 cacctggaat ggcggcacac gctcgaacct cttcgtggcg ccgatgaaca ccgacggccg
     1561 cgccggaacg ccgctcaacg tgagcaaggc gctcgacgcc gacgtcccgt ccaagcccga
     1621 cgggggcgac gaggaataca cgttcagccc cgacggatcg cgcgtggtgt tcagcgcacg
     1681 cgtagccgga cgcgaggaag cgtggtcgac gaatttcgat ctgtacgaag tgccgtcgga
     1741 cggcagcgcc gccccgcgca acctcactgc caacaatccc gcctgggaca cgcagcccgt
     1801 cttcctgcgc aacggcgatc tggcgtggct cgccatgagc cgcccgggat tcgaggccga
     1861 ccgctttcgc atcatgatca gaagcgacgg cgaagtgagc gaagtcgcgc cggactggga
     1921 ccgctcggtg caacacctcg acgtggcgca cgatggccgc acgctgctcg ccaccgcgag
     1981 cgatctggga caaacaccct tgtttcgcgt ggacgtggac agcggccgcg tgacgcccgt
     2041 ctcgggcaaa ggttacgtcg gctccttcgc gaccacgcgg cgtggcaccg tggtgctgtg
     2101 gcacgatctc gcctcgcccc ccgatctcta tctactgccg aacagcggcg agcggcgccg
     2161 gctgacgaac gcgaattcgg aaatcctctc ggcccgcacg ctcggtgcgt tcgagcaatt
     2221 cagtttcccg ggctggaaca acgaaacggt gtacggctac atcgtgaatc cgcccggcct
     2281 ggacaaggac cgcaaatacc cggtcgcctt catcgtgcac ggtggtccgc aggtgagttt
     2341 ccagaatcac tggaactggc gctggaatgc gcagactttc gcggcacgtg gctacgcgac
     2401 ggtcttcatc gattttcacg gctcgccggg ctacggccag gcattcaccg actcgatcag
     2461 ccagcactgg ggtgaccgtc cgttcgaaga cctgaagaaa ggtttcgccg cggccgtgca
     2521 gaaattcccc tggctcgacg gccagcgcgc gtgttcgctc ggcgcctcct acggcggcta
     2581 catgcagaac tggatcgccg gcaactggcc cgacggtttt cgctgcatcg tcaaccacga
     2641 cggcattttc gacacgcgct ccatgtacta cacgaccgag gagctgtggt tcacggaatg
     2701 ggagaacggc gggccttact accaggcgcc cgagatccac gagcgcttca accccgcgca
     2761 ccacgtgaag aactggcgca cgccgatgct ggtgatccac ggcgagcagg atttccgcgt
     2821 gcccgacacg cagggtattg ccacgttcac cgcgctgcag cggttaggcg tggaaagcag
     2881 gttgctgtat ttccccgacg agaatcactg ggtgctgaag cccaacaaca gcattcagtg
     2941 gtacggcacg gtgctcgact ggctggacgc gcacacgaaa tgattacagg cgccggaaaa
     3001 gctccgcggg agatccgccg cacgagtaga tgaccgagtt cagatcttcg gtcggctgga
     3061 tgtcgcaggt gcccgcgggc ctgacgtctt tcggcagcag cgcggtgaac agctccgggt
     3121 tttcacgcag gttgcgccac tggccttcct gcgtgaacgc cagcgcggcc tgcgtcacct
     3181 tcttgccgtc gggggaaatc ttgaggtcga gcaggcggcc gaagatgccg ttggcgtcgt
     3241 gacgctcgat gtacatggac tcgtcggtca ccatgcgcga acggaccggc acgatgtaca
     3301 gcttcgcgcc gctgtcgctt tcatattgcc ccgaggtgtt ccgctggaag tcggcgatgg
     3361 tctgcatgtc cctttgctga ggagtggtgc agccgcagag cacggcgacg agcgcgagcg
     3421 ggccggcgag acgttgcgcg gtgttcatga cagacctctc aaccgtaata ggcgcgccac
     3481 tctgcctgat aaccggcgcc gagcgtaagc agtttctcga gtagctcggg gtaactcacg
     3541 ccggcggcga gcgccgactg cgcgaagtcc tcgtccgccg cgaggttggg attgggattg
     3601 gcctcgatga cgtagatctg cccttcggcg ttcatgcggt agtcgatgcg cgcgcaaccc
     3661 gagagcccga ggctgcgata gacgcgccgc gatagtttgt cgagacgcgc cacgatcgcc
     3721 ggggaaagtt ctttcgcctc gtacgtgtcg atgccgtgtt tcttctggta tttcttgtcc
     3781 cacttcacgc gccgcgtcgc gatgcccgcc gagttctcgg ccagcgatcc gaatttcatc
     3841 tcccacaccg gcaaccgcgt gagccggtcg ttgcccatca cgcccacgta cagctcgcgg
     3901 ccctcgatgt attcctcgac cagcgcgtcg gtctgcacct ggtcgtgcac gaactggatg
     3961 cgctgcttga gctgcgcctt gtcggtgacg atggaagcct gcgaaatccc gagcgaagcg
     4021 tcttcggtcg
//
LOCUS       DATDEU010000715         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1742354, whole genome shotgun sequence.
ACCESSION   DATDEU010000715 DATDEU010000000
VERSION     DATDEU010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742354"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VIV63_17065"
     CDS             <1..1036
                     /locus_tag="VIV63_17065"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY6126367.1"
                     /translation="IHKLTGATPTVSLHLPWDRTDDYQGLKEHGAALGLGFDAMNSNT
                     FQDQAGQPLSYKYGSLTHTDPAVRAQAVAHNIDCIAAGKALGSRALTVWIGDGANFPG
                     QSHLGRAFDRYLESQKQIYAALPDDWRMFIEHKMYEPAFYATVIQDWGSSLMAAQTLG
                     PKAFCLVDLGHHAPIVNIEMIVARLVHAQRLGGFHFNDSKYGDDDLDAGSIEPFRLFL
                     VFNELVEAELGKASNFKPAHMLDQSHNVTDPIESLMTSAIELQRAYAQALIVDRKALH
                     EYRLGNDVLMAAQTMKRGFTTDVSPILAQARVSRGAAIDPVGAYRQSGYRAKCGQMRP
                     GKQSLGGGIV"
     gene            1061..>2091
                     /locus_tag="VIV63_17070"
     CDS             1061..>2091
                     /locus_tag="VIV63_17070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF008398.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY6126368.1"
                     /translation="MTVLNSIEDLRQAARRRVPRAIFDYADRGSYDEVTFNRNLADLA
                     AIQLRQRVMVDVSKQQLATTILGEQWTIPVGIGPTGLTGLFHANGEMLGARAAQDFGV
                     PFCLSTMSICSIEDVRGAVQKPFWFQVYLMRDRSFNQELLDRALAANCSAIMLTVDMP
                     VQGLRRRDPKNGLSIPPKLTLKNMFEVAMRPTWALKVLTGRRRTFGNLEARMKGTGGL
                     KTLSEWISQQFDPTVTWKDLAWLRERWPRKIIIKGILDPEDARLAVEHGVDAMVVSNH
                     GGRQLDGARSTISALPGVVDAVAGRSEVLFDGGVRCGQDVLKALALGANGCLIGKAFL
                     YALAARGEAG"
BASE COUNT          380 a          691 c          657 g          363 t
ORIGIN      
        1 catccacaaa ctcacgggcg cgactcccac cgtatcgctg catcttccct gggatcgcac
       61 cgacgattac cagggcctca aggaacacgg cgcggctctg ggtctcgggt tcgatgcgat
      121 gaactcgaac accttccagg atcaggccgg gcaaccgctg tcgtacaagt acggcagcct
      181 cacgcacacc gaccccgcgg tgcgcgcgca ggcggtggcg cacaacatcg actgcatcgc
      241 ggccggaaag gctctcggct ccagggcttt gacggtgtgg atcggcgatg gcgccaactt
      301 tccgggccag tcccaccttg gccgcgcgtt cgatcgctat ctcgagtcgc agaagcagat
      361 ctacgcggcg ctgcccgatg actggcgcat gttcatcgag cacaagatgt acgagccggc
      421 gttctatgcc acggtcatcc aggactgggg cagctcgctc atggcggcgc agacgctcgg
      481 ccccaaggcc ttctgcctcg tcgacctcgg acaccacgcg cccatcgtga acatcgagat
      541 gatcgtcgcg cgcctggtgc acgcgcagcg gctcggcggt ttccatttca acgattcgaa
      601 gtacggcgat gacgatctcg acgcgggctc gatcgagcct ttccggctgt tcctcgtgtt
      661 caacgagctc gtggaagcgg aactcggcaa ggcgtcgaac ttcaagccgg cgcacatgct
      721 ggatcagtct cacaacgtca ccgacccgat cgaaagcctc atgaccagcg ccatcgaact
      781 gcagagggcc tatgcgcagg cgttgatcgt cgatcgcaag gcgctgcacg aataccggct
      841 cggcaatgac gtgctcatgg cggcgcagac gatgaagcgc ggcttcacca ccgacgtgtc
      901 gccgatcctc gcgcaggcgc gcgtgagccg gggcgcggcc atcgatccgg tgggtgccta
      961 tcgccagagc ggctaccgcg ccaagtgcgg acaaatgcgg ccgggcaaac agagtctggg
     1021 tggtggcata gtctaggaaa ggggacgcac ctcaacacct atgaccgtac tgaacagcat
     1081 cgaggacctg cgtcaggccg cacgccgccg cgtccctcgc gccatcttcg actacgccga
     1141 ccgcgggtcg tacgacgaag tcactttcaa ccgcaatctc gcggatctcg ccgccatcca
     1201 gcttcgccag cgcgtcatgg tcgacgtctc gaagcagcag ctggccacca ccattctcgg
     1261 cgagcaatgg accattccgg tcggcatcgg gccgacgggt ctcaccgggc tgtttcacgc
     1321 caacggcgaa atgttaggcg cgcgcgccgc gcaggatttc ggcgttccgt tctgcctgag
     1381 cacgatgtcc atctgctcca tcgaggacgt gcgcggcgcg gtgcagaagc cgttctggtt
     1441 ccaggtctat ctgatgcgcg accgcagctt caatcaggaa ctgctcgatc gcgcgctcgc
     1501 cgccaattgt tccgccatca tgctcaccgt cgacatgccg gtgcagggcc tgcgccggcg
     1561 cgacccgaag aacggcctgt ccattccgcc caagctcacg ctgaagaaca tgttcgaagt
     1621 ggcgatgcgg cccacgtggg cactcaaggt cctcacggga cgccgccgca ctttcggcaa
     1681 cctcgaagcg cgcatgaaag gcaccggtgg tctgaagacg ctgtccgagt ggatctcgca
     1741 gcaattcgat cccacggtca cgtggaaaga tctcgcgtgg ctgcgcgagc gctggccgcg
     1801 aaagatcatc atcaagggca ttctcgatcc cgaggacgcc agactcgccg tggagcatgg
     1861 agtggacgcg atggtggttt cgaaccacgg cggccgccag ctcgacggtg cgcgttccac
     1921 gatctcggca ctgcctggcg tcgtggatgc cgtcgccggc cgaagtgaag tcttgttcga
     1981 cggtggtgtg cgctgtggcc aggacgtgct caaggcgctg gcgctcggcg ccaacggctg
     2041 tttgatcggc aaggcgtttc tctatgccct cgccgcgcgc ggcgaggcgg g
//
LOCUS       DATDEU010000716         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_137922, whole genome shotgun sequence.
ACCESSION   DATDEU010000716 DATDEU010000000
VERSION     DATDEU010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137922"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VIV63_17075"
     CDS             <1..923
                     /locus_tag="VIV63_17075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6126369.1"
                     /translation="EILPWTDSGARDGDVTFLHFAIPSPMTAAFARLPGARALYYHNV
                     TPPRFFAPFDAGLAAFCARGRQELATLVGRVDLAIGVSDYNRRELEQLGFAPTAVLPI
                     IVDFGRLRSAPPVPAIETTLQDGLVNILFVGRIAPNKKIEDHIKLAEQFKRYVDVYYR
                     FIFVGKYDAVPRYHYAVRALIGEFKMLPDRFWFTGPVPDIELAAYYRNAHAYVSMSEH
                     EGFCVPLVESMAMDVPVLAYGAAAVPETLGGAGVTFAPKDIEYAAELLGGLIYDLPFR
                     EQVIEGQRRRVRDFAQDRIEPMVEDVLEML"
     gene            920..2374
                     /locus_tag="VIV63_17080"
     CDS             920..2374
                     /locus_tag="VIV63_17080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6126370.1"
                     /translation="MRLAFIVQRYGTEILGGSEYHCRLIAERMADRHQVDVLTTCARD
                     YITWENVYPEGTDRIRGVTVRRFANARTRDIEAFNRTSDWIFHNSHNRQDELEWLKQQ
                     GPWSPGLIEYLERQHAQYDVLIFFTYLYAPTVMGIRVAPSKSLLVPTAHDEPAIRLGI
                     YKDVFSSAAGIVWNTEAEKRFIASSFHLRALVEDIVGCGVDMPEGEAVAAGDDRMGLA
                     PTSREPLPAHIEGRANTFRRRHRLYGDFLLYGGRIDPGKGCEELFEYFQTYLKDGGDT
                     TLMLMGTKLMPLPEDPHVRFAGTLPDEDRLHALEAATVVVVPSPHESLSLLALEAFAV
                     GTPILANARSEVLVDHCRRSNAGLYYADRFEFIEALKVLMKDAPLRESMGRSGQAYVN
                     RYYRWSAIMAKYERLFARVRGGTGAEREGDRERPRVIAREADRERGRSGATGRDRAGR
                     PARDGRDRPRGDRARDGRGRGDRGRGGDARRGQR"
BASE COUNT          422 a          865 c          860 g          429 t
ORIGIN      
        1 acgagatcct gccgtggacg gattcagggg cgcgcgatgg cgacgtcacg ttcctgcact
       61 tcgccatccc gtcgccgatg acggccgcgt tcgcacggct acctggcgca cgggcgctct
      121 actaccacaa cgtcacgccg ccacggttct tcgcgccgtt cgacgcgggt ctggccgcgt
      181 tctgcgcgcg gggccggcag gagctggcga cgctcgttgg ccgggtggac ctggcgatcg
      241 gcgtctcgga ctacaaccgt cgcgaactgg aacagctcgg cttcgcgccg acggccgtgc
      301 tgccgatcat cgtcgacttc ggccggctgc gatccgcacc gccggtgccg gccatcgaga
      361 cgacgctgca ggacgggctg gtgaacatcc tgttcgtcgg ccggatcgcg cccaacaaga
      421 agatcgaaga ccacatcaag ctggccgagc agttcaagcg gtacgtggac gtgtactatc
      481 gcttcatctt cgtgggcaag tacgatgcgg tgccgcgcta ccactacgcc gtgcgcgcgc
      541 tcatcggcga gttcaagatg ctgccggatc gcttctggtt cacgggcccg gtgccggata
      601 tcgagctggc agcgtactat cggaacgccc acgcctatgt ctcgatgagc gagcacgaag
      661 ggttctgcgt gccgctggtc gaatcgatgg caatggacgt gccggtgctg gcgtacggcg
      721 cggcggcggt ccccgagacc cttggagggg ccggagtgac gttcgcgccc aaggacatcg
      781 agtacgccgc cgaactgctc ggcggcttga tttacgacct cccgttccgg gagcaggtga
      841 tcgaaggcca gcgccgccgt gtgcgcgact tcgcccagga ccggatcgag ccgatggtcg
      901 aagacgtgct ggagatgctg tgagacttgc cttcatcgtg caacgctatg gcaccgagat
      961 cctgggcgga tccgagtacc actgccggct catcgccgag cggatggccg accgccacca
     1021 ggtcgacgtg ctcaccacgt gcgcacgcga ctacatcacc tgggagaacg tctacccgga
     1081 gggcaccgac cgcatccgcg gcgtgacggt gcggcgcttc gccaacgcgc gcacgcgcga
     1141 tatcgaggcg ttcaaccgca cgtcggactg gattttccac aacagtcaca accggcagga
     1201 cgaactcgag tggctgaagc agcaggggcc gtggtcgccc ggcctcatcg agtacctcga
     1261 acgccagcac gcgcagtacg acgtcctgat tttcttcacc tacctctacg cacccacggt
     1321 gatgggcatc cgggtggcgc cgtccaagag cctgctggtg cccaccgcgc acgatgaacc
     1381 cgccatccgg ctcggcatct acaaggatgt cttttcgagc gccgcgggca tcgtctggaa
     1441 caccgaggcc gaaaagcgct tcatcgcctc gtcgttccac ctgcgcgcgc tggtcgagga
     1501 catcgtcggg tgcggcgtcg acatgccgga aggcgaggcc gtggcggccg gtgatgaccg
     1561 gatgggactc gctccgacca gccgggaacc gctgccggcg cacatcgaag gccgcgcgaa
     1621 cacgttccgt cgccggcatc ggctctacgg ggatttcctc ctgtacgggg gccgcatcga
     1681 tcccggcaag ggctgcgaag aactcttcga gtacttccag acctacctca aggacggcgg
     1741 cgacacgacg ctgatgctga tgggcaccaa gctgatgccg ctgccggagg atccgcacgt
     1801 gcggtttgcc ggcacgctgc cggacgagga ccgtctgcat gcgctcgaag ccgcgactgt
     1861 cgtggtcgtg ccatcgccgc acgagagcct ctcgctgctg gcgctggaag cgttcgcggt
     1921 cggcacgccg attctcgcca acgcacgctc ggaggtcctg gttgatcact gccgccgcag
     1981 caacgccggc ctgtactacg cggaccgctt tgagttcatc gaagcgctga aggtgctgat
     2041 gaaggacgcg ccgctgcgcg agtcgatggg ccgcagcggc caggcttacg tcaaccggta
     2101 ctaccgttgg agcgcgatca tggcgaagta cgagcgcctg ttcgcgcgcg tccgcggcgg
     2161 cacgggcgct gaacgcgagg gcgaccgaga gcggcctcga gtgatcgcgc gcgaggccga
     2221 tcgtgaacgc ggacggtcgg gcgcgacagg ccgcgaccgc gccggccggc cagcacgtga
     2281 cggccgcgac cgcccacgcg gagaccgcgc ccgggacgga cgtggccgcg gcgatcgtgg
     2341 acgcggtggt gacgcgcgac gcggacagcg ctgatgcgcg ggtaacctga cgctctctcc
     2401 gtgttctccg cgtgaatatc caagcccgtg cccgcctttt ccgcgcctcc gcgtgatcaa
     2461 gcttgcgatc aaggggtcgt acctgcctac cggcaagcga ggtacgaccc ctctattctt
     2521 cgatgatctc tacttcgtcg atctcggtct cggtctccgc ttcgccgttc gtccgc
//
LOCUS       DATDEU010000717         4071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_722650, whole genome shotgun sequence.
ACCESSION   DATDEU010000717 DATDEU010000000
VERSION     DATDEU010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722650"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..622)
                     /locus_tag="VIV63_17085"
     CDS             complement(86..622)
                     /locus_tag="VIV63_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126371.1"
                     /translation="MKTGRILLTLALAAAAAAPLMPHAPAPQVVGQSGFPGWPREFEG
                     VALERIPPSAQDAFFTRDFPGRVARFAARDRQIVIRWVSSPTRRLHPASHCFTGAGYA
                     VDPRPMRRSRDGELMGCFAARKNGETLHVCEQLRDSTGASSPDVSSWYWRALIAPAGN
                     SWWSYVSVEREEGGVRTP"
     gene            complement(619..1410)
                     /locus_tag="VIV63_17090"
     CDS             complement(619..1410)
                     /locus_tag="VIV63_17090"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HEY6126372.1"
                     /translation="MNPLLALLAVAVACWSAWRELAARLPEASDAMPLALVVAALAVP
                     CLRGTARLGSASRVPTVPLVVLLCLYTLAVIIAPPLLRIAPAVLAVCYCLHAASQDGA
                     PPASFYGLVLLALPVLPSLEFYTAYPVRLAAIEATAALLRMNGIAVGVDGLALRFGEQ
                     LIQFDAPCSGVRMLWAGWFFASALAHLYRLTWWRYVAALAIATGLAIGGNIVRASSLF
                     YLEAKLLPFAEAAWMHEAVGTVAFGMTALLVFLAVRPRRVAQCPA"
     gene            complement(1407..3815)
                     /locus_tag="VIV63_17095"
     CDS             complement(1407..3815)
                     /locus_tag="VIV63_17095"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY6126373.1"
                     /translation="MEAPPFAGRHTNPSRFENAGRHLKFGFSLFAGFLLPAFTIAFEL
                     ITRMCAETLFDPLPSLAHLAVVTAVPLINLKLLLMRKREQPIARGWLFSGAAAAAVGL
                     SYALLFLPVYPFAVIGIIFFGLGLLPFAPLGAGLTALFVTVGVARNAQRPYLRLMICG
                     LLAGLVLVLALDAPTAITRAAVRGSVHADPAVRARSVILMRNFGNRELLLRLCYDSSG
                     HTGGLLGLLVEGTLSLQIDDFDRRRLIDSPSAARELYYRINGVPYNTVAPPYRGRGWR
                     FADDFAWDADQGGTVIGGRVSGLSLSSSRIDGSMDADDAVAYVEWTTEFTNTSSAQRE
                     ARMSLVLPPGGVVSRATLWVNGEEREAVFATRAAARAAYQAVVMQRRDPLLITTNGAD
                     QVFVQMFPVPPGGTAKFRIGISAPLALTNDGRATLALPAIVDRNFSIDAALRHAVWIE
                     GEGSEANADSGFSKIAGESGIVRQRGNFTDQDLAIRRPRISIARNPKAEEIASGTVMQ
                     TIRREPREPARSFYVVLDGSLQAKPARHALLGALEHIPVGARFGFAMASATPGTLPLA
                     PWSAARRQELTTLLERQSFDGGEDNLGALAEGIAALQGEPGATLLWIHGPQGFEFDEF
                     TGQLEQVLDRGTHLPEIWLLPVAPGPNKALQHPRAFQLAHTLAWSGDTQADIAAAITN
                     YFDTAPRWTVRRAPGPPPGLVVGSQHIEKLWALEQIESLMTQGSDGAARAMALAAQHR
                     LVTPVSGAVVLETDVEYTRAGLTPPDVAAVPTIPEPSTWVLLIVAALAFAWAVRQQRR
                     RALA"
BASE COUNT          750 a         1349 c         1363 g          609 t
ORIGIN      
        1 cccaggtgac ttcgccgcgt tccgtctcaa gcccactcga aagcgactct ggtaaaaccc
       61 gacccaatgc gtggagtgcg gttcgtcaag gagttctgac cccgccttct tcgcgttcga
      121 cgctcacgta cgaccaccag ctgttgccgg cgggcgcgat gagcgcgcgc cagtaccagg
      181 atgaaacgtc gggcgagctg gccccggtgg aatcgcgcag ttgctcgcag acatgcaacg
      241 tttcgccgtt cttgcgagcg gcgaaacaac ccatcagttc accgtcccgc gagcgacgca
      301 tcggccgcgg gtcaacggcg taaccggcgc cggtgaagca atgactcgcc ggatgcagac
      361 ggcgcgtcgg cgaactgacc cagcggatga cgatctgccg gtcccgcgcc gcaaagcgcg
      421 ccacgcgccc cgggaagtcg cgcgtaaaga aggcgtcctg cgcggatggt ggaatgcgct
      481 cgagcgcgac gccctcgaac tcgcgcggcc atcccggaaa cccgctttgc ccgacgactt
      541 gcggcgcggg tgcgtgtggc atcagcggcg ccgccgcggc ggccgcgagg gcaagcgtca
      601 gcaatatcct gcccgtcttc atgcggggca ctgcgcgacg cggcgcggcc gcacggccag
      661 gaacaccagc agcgcggtca ttccgaaggc aacggtcccc acggcctcat gcatccatgc
      721 ggcttccgcg aaaggaagga gtttggcctc gagataaaac aggctggaag cacgcacgat
      781 gttgccgccg atcgcaagcc cggtcgcgat cgccagcgcg gccacatagc gccaccacgt
      841 gaggcggtac agatgggcga gcgcgctcgc gaaaaaccag ccagcccaca acatgcgcac
      901 gccgctgcag ggcgcgtcga actggatcag ctgctcaccg aatcgcagtg ccaggccatc
      961 gacacccact gcgatgccgt tcatgcgcag cagtgccgca gtcgcttcga tggccgcgag
     1021 gcgcacggga tacgcggtgt agaactcgag tgatggcagc accggcaacg cgagcagcac
     1081 gagcccgtag aacgacgcgg ggggcgcgcc gtcctgcgaa gccgcatgca ggcagtaaca
     1141 cacggcgagc actgcgggcg cgatgcgcag cagcggcggc gcgatgatga cggccagcgt
     1201 gtagaggcac aggaggacga cgagcggcac cgtcggcacg cgcgaggcgc tgccgagtcg
     1261 cgcggttccg cgcagacacg gcacggccag cgccgccacg acgagcgcga gtggcatcgc
     1321 gtcagaagct tcgggcagac gcgcggccag ttcgcgccag gcgctccagc aggcaaccgc
     1381 gaccgcgagg agtgcgagca gcgggttcat gcaagtgcac ggcggcgttg ttgtcgcacc
     1441 gcccaggcga atgcgagagc ggcgacgatc agcaacaccc aggtggaagg ctccggaatc
     1501 gtggggaccg ccgcgacatc cggcggtgtg aggccggcgc gggtgtactc cacgtccgtc
     1561 tccagtacga ccgcgccgct caccggcgtc acgagacgat gttgcgcggc cagggccatg
     1621 gcacgtgctg ctccgtcact gccctgggtc atcagggatt cgatctgctc cagtgcccag
     1681 agcttctcga tgtgttgcga gccaacgacc aggccgggtg gcggccccgg cgcgcggcgc
     1741 acggtccagc gcggcgcggt gtcaaagtag ttagtgatcg ccgcggcgat gtctgcctgg
     1801 gtgtcaccgc tccaggccag cgtgtgcgcc agctgaaagg cccgtggatg ctgcaacgcc
     1861 ttgttgggcc ccggagctac gggtaagagc cagatctcgg gcagatgcgt gccacgatcg
     1921 agcacctgct cgagctggcc ggtgaattcg tcgaactcga agccctgcgg cccgtgaatc
     1981 cacagcaacg tggcgcccgg ttcgccctgg agtgcggcga tgccttcggc aagcgcgccg
     2041 agattgtcct cgccgccatc gaatgactgg cgctcgagca gcgtggtgag ttcctgtcgg
     2101 cgggcggcac tccagggcgc gagcggcaag gtgccgggag tcgccgacgc catggcaaac
     2161 ccgaaacggg cgcccacggg aatgtgctcg agcgcgccca gcagggcgtg acgcgccggt
     2221 ttggcttgca acgagccgtc gagcacgacg tagaacgaac gggcgggttc gcggggctca
     2281 cgccgaatcg tctgcatcac ggtgcctgac gcgatctcct ccgccttcgg gttgcgcgcg
     2341 atggaaatgc gcggccggcg gatcgcgaga tcctgatccg tgaagttgcc gcgctgccgg
     2401 acgatgccgc tttccccggc gatcttactg aaccccgagt ccgcgttggc ctcgctacct
     2461 tcgccctcga tccacaccgc gtggcgcagc gctgcatcga tggaaaaatt ccgatccacg
     2521 atggccggta gcgccagcgt cgcacgaccg tcattggtca gcgcaagtgg cgccgagatg
     2581 ccgatgcgaa acttcgcggt gccgcccggc ggcacgggaa acatctgcac gaacacctgg
     2641 tccgcgccgt tggtggtgat cagcagcgga tcgcgccttt gcatgaccac cgcctgatac
     2701 gcggcgcgtg ccgcggcgcg cgtggcgaat acggcctcgc gttcttcgcc gttcacccag
     2761 agcgtggcgc gcgaaaccac gccacccggc ggcaacacca gcgacatgcg cgcctcgcgc
     2821 tgcgcggaac tcgtgttcgt gaactcggtg gtccactcca catacgcgac ggcgtcgtcc
     2881 gcatccatcg agccgtcgat gcgcgagctg ctcagcgaaa gtccgctgac gcgtccgccg
     2941 atgaccgtgc cgccctgatc ggcatcccag gcgaaatcgt ctgcgaatcg ccagcctcga
     3001 ccgcgatacg gcggagccac cgtgttgtaa ggcactccgt tgatgcggta gtagagctct
     3061 ctcgcggcgc tgggggagtc gatgaggcgg cgccgatcga agtcatctat ctgcagactg
     3121 agcgtgcctt caaccaacaa cccgagcagg ccgccggtgt ggccactgga gtcgtagcac
     3181 aggcgcagca gcagttcgcg attgccgaaa ttgcgcatga gaatcacgga gcgcgcgcgc
     3241 actgcgggat ccgcgtgcac actgccgcgc acggcggcgc gggtgatcgc ggtgggcgca
     3301 tcgagcgcca gaaccagcac gagcccggcc aggagtccgc agatcatcag tcgcaggtac
     3361 gggcgctgcg cgttccgggc gaccccgacc gtgacgaaca atgcagtgag gcccgcgccc
     3421 aatggcgcga acggaagcag gcccaagcca aagaagatga ttccgatgac cgcgaaggga
     3481 tacacgggca ggaacagcag cgcgtacgac aagcctaccg cggctgccgc ggcccccgag
     3541 aacagccaac cgcgagctat cggctgctcg cgcttgcgca tcagcagtag tttgagattg
     3601 atgagcggca ccgcggtgac gacggcgaga tgcgcgaggg atggcagcgg atcgaacaac
     3661 gtttccgcgc acatccgcgt gatgagttcg aaggcgatcg tgaaagcggg cagcagaaat
     3721 cccgcgaaaa gactgaaacc gaatttcagg tggcggcccg cgttttcaaa ccgcgacggg
     3781 ttcgtatgcc ggccggcgaa cggtggcgct tccatccatt actccctctc gtttttattg
     3841 tcggagatac tggaactgca ttacggcggc tcgatggatc aattgtggcg aattatttct
     3901 ggatcgtccc gggccagcgg caattcgtcg cctggtcctg accgttgtga gcagcaggcc
     3961 ggcgaccgag gcacatcaca tccgcttgca acccgtcgca gccgccgtcc gtcttacccc
     4021 catgaaacgc acactactag ccctcagcgc cctgttcttc tcctgctttc t
//
LOCUS       DATDEU010000718         5949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_138263, whole genome shotgun sequence.
ACCESSION   DATDEU010000718 DATDEU010000000
VERSION     DATDEU010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138263"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VIV63_17100"
     CDS             complement(<1..859)
                     /locus_tag="VIV63_17100"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6126374.1"
                     /translation="MTDERLNNIVIVGGGTAGWMAAAGLAHALAGTCAIRLVESDDIG
                     TVGVGEATVPHLRSFNASLRIDEIEFVRAVRGTFKLGIQFVDWGRIGDSYVHGFGSIG
                     HDYRALPFHQYWLKLHLAGKARELGDYSINTAAAPAGKFLSGADDVPKDSPLSQVAYA
                     YHFDAGLYAQYLRRYAEARGVKRTEGEVVDVTLRGEDGFIESVTLKSGARITGDLFID
                     CSGFRGLLIEQALHTGYENWSHWLPCDRAIAVPSEKTGPATPYTRSTARAAGWQWRIP
                     LQHRTGNGYV"
     gene            complement(885..3854)
                     /locus_tag="VIV63_17105"
     CDS             complement(885..3854)
                     /locus_tag="VIV63_17105"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6126375.1"
                     /translation="MNNFPRTILGAAIAAVLATPVITWAQSADAALRGKAPANTAITA
                     KNVATGATRRTQANAEGIYSLTGLPPGTYTVDAGPGTATTVTLSVASTATLDLIATSG
                     AVEAPDAPMQEITVQGRRLQEVRTSEVGSTVSEHQIETVPQITRNFLEFADTIPGVVF
                     EVDGSGRTKISGGAQNRNGVNLYIDGVGQKGYVRSGVSGQAGDTQGNPFPQLAIGEYK
                     VITSNYKAEYDQISSAAITALSRSGTNTFSGEAFGTYSADNFRAKTPAELDSGRKAES
                     ESKEYGVAFGGPIIEDKLHFFVTYEGKRFVTPVSVLPAFAPSDVVAQLPAGALAQLGP
                     TSIDFEEDLFFAKLDWAMSERDSLQFSAKVRDEVSSGDRTGVGIARSATVDTNNDDTR
                     LELSWKHSADSWLNELQLTYEDAFYVPQITNSDVNGAVYTWFDGQDRNVLAVDGADPR
                     AGQNKGQKGWAIGNFITFSDLAWGAGDHTIKAGVKYKDVDLTAADSVPGNPVFYYDVT
                     PAGTATVPWKTAFALPLAGFDSEVTTNDKQLGLFVQDDWTVNDHLTLNLGIRWDVEWN
                     ESYLDFETPQFLLDALNTEVSPGVTYGQSLGLSTDPNVAIDINDYISTGNNRDPQQDS
                     FQPRFGFSYDLAGDQAHVIFGGAGRAYDRTLYDYLQLEQTKFALATAEVRFNTADHPC
                     TVNGSNCVNWDPAFASDPGALLALLNGMAGEVNLINNDLKVPYSDQFSLGMRNRLGDW
                     NTSASVTRVLSHDGFVFTMGNRYPNGDFWQNRGQPWGNPPPGLAGVLLVGDTGIETKS
                     TQILLSADKPFTQDSRWGASFSYTYTDAEHNRDINEHYLFDESSIEPFPFILSNAAAK
                     HRLVSTGSYAAPWGFMIAGKLTLSTPIPRNVISCYAGTATFPTGAPCNPFAVVADDTL
                     GYKSLDIQITKNFEIGDLGSMYVRIDALNVTNEHNLVDYIDTSGPDGIITDTRYAPFG
                     NITGVPRTIRMSLGVKF"
     gene            complement(4080..>5949)
                     /locus_tag="VIV63_17110"
     CDS             complement(4080..>5949)
                     /locus_tag="VIV63_17110"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY6126376.1"
                     /translation="DARVVAGISGLEGYSHGQRWTFAPMVDHPADPRWGRVVETFGES
                     PLVSSDYAVATIRGFHGSLAQLPGKPAPVDFGIASCLKHYLGYGAVQGGKDYAYVDLT
                     ERSIREFHLPPFAAGVAAGAPSVMPAFTTGPGGVPMSAHRKLLQDVLRGELGFNGVLV
                     SDYAGITEMRNHGTAADDLEAAVQSLREGTMTVDMEDGVYYAQLARAVAAGRLREAEV
                     DREVLRALAFKRRLGLFEKPYVPEDLEKRVRVSAEHRAAAREIARKSIVLLKNDANLL
                     PLHARRILVTGPLADAREDLLGPWHARGEAKDVISVLTGIRQRGASAKPAVTVSYSEG
                     AQIDGKGKAVDGDAQRISAAVSEAHNSDLIVAVVGERESQSGEAKNRAYLDLPGSQQA
                     LVDALVATGKPVVVVLITGRALAVPKLAEQSGALVHSFFPGIEGGNALADVLFGDYNP
                     GGKQPVTWPRSVGQLPIHHYDPPNGRPNIPERGDYKAHWLDESDDPLFAFGYGLSYTR
                     FSLSALELPKRIGPADTLTIRVRLTNHGDRAGDEVPQLYIRPRVASGVTGQRLQAYRR
                     VHLAAAESKIIEFTLPAKSLVTMNSQDRWALESGTYEVTLGTSARGGLTGTFELTN"
BASE COUNT          983 a         1838 c         2011 g         1117 t
ORIGIN      
        1 agacgtagcc gttgccggtg cgatgctgaa gcgggatacg ccattgccag cccgcggcgc
       61 gcgccgtcga gcgtgtatag ggagtggccg gcccggtctt ttcgctgggc accgctatcg
      121 cgcggtcgca gggcaaccag tggctccagt tctcgtaacc cgtgtgtaaa gcctgttcga
      181 tcagcaggcc gcgaaatccc gaacaatcga tgaacaggtc gccggtgatg cgcgcgccgc
      241 tcttcagggt gaccgattcg atgaagccat cctcgccgcg cagcgtcacg tcgaccactt
      301 cgccttcggt gcgcttcacg ccgcgcgcct cggcgtagcg gcgcagatac tgcgcgtaca
      361 gccccgcgtc gaagtgatag gcgtaggcca cctgggacag cggtgaatcc ttcgggacgt
      421 cgtcggcccc tgaaaggaat tttccggccg gggccgcggc ggtgttgatg gaatagtcgc
      481 cgagttcccg cgccttgccg gcaagatgaa gcttgagcca gtactggtgg aagggcagtg
      541 cgcggtagtc gtggccgatg ctgccgaagc cgtgcacata cgaatcgccg atgcggcccc
      601 agtccacgaa ctggatgccc agcttgaacg tgccgcgcac cgcgcgcacg aactcgattt
      661 cgtcgatgcg cagcgatgca ttgaacgagc gcaggtgcgg gaccgtcgct tcgccgacgc
      721 ccaccgtgcc gatgtcgtcc gactcgacga gccggatcgc gcacgtgccc gcgagtgcgt
      781 gcgcgagacc ggcggcggcc atccagccgg cggtgccacc acccacgatg acgatgttgt
      841 tcagcctttc gtcggtcatc gcgccgattg tggcaccgat cccgtcagaa cttcacgcct
      901 aacgacatgc ggatcgtgcg gggcacgccc gtgatgttgc cgaaaggcgc gtaccgggtg
      961 tccgtgatga ttccgtcggg tccgctggtg tcgatgtaat cgacgagatt gtgctcgttg
     1021 gtcacgttga gcgcgtcgat ccgcacgtac atcgacccga gatctccgat ctcgaagttc
     1081 ttcgtgatct ggatgtcgag cgacttgtaa cccagggtgt catccgccac gaccgcgaac
     1141 ggattgcagg gcgcgccggt cggaaacgtc gccgtccccg cataacagga gatgacgttt
     1201 cgagggatgg gcgtcgacaa cgtcagcttg cccgcgatca tgaatcccca gggcgccgcg
     1261 tatgaacccg tggagaccaa ccggtgtttg gcggcggcgt tcgacaggat gaaggggaac
     1321 ggttcgatcg acgactcgtc gaatagatag tgctcgttga tgtcgcgatt gtgttccgca
     1381 tccgtatacg tgtacgagaa gctggctccc cagcgcgagt cctgcgtgaa tggcttgtcc
     1441 gccgaaagca ggatctgcgt gctcttcgtc tcgatgccgg tgtcgccgac cagcagcacg
     1501 cccgcgaggc cgggaggcgg gttgccccag ggctgtccgc ggttctgcca gaaatcgccg
     1561 ttcggatagc ggttgcccat cgtgaacacg aagccatcgt ggctcagcac gcgagtgacc
     1621 gaggcgctcg tgttccagtc cccgagacgg ttgcgcatgc cgaggctgaa ctgatcggag
     1681 tagggcacct tgaggtcgtt gttgatgaga ttgacctcgc ccgccatgcc attgagcagc
     1741 gccagcagcg cgccggggtc gctcgcgaat gccggatccc agttgacgca gttggagccg
     1801 ttcaccgtgc agggatgatc cgcggtgttg aagcggacct ccgcggtcgc gagggcgaac
     1861 ttggtctgtt cgagctgaag gtagtcgtac aacgtgcggt cgtaggcgcg tccggcgccg
     1921 ccgaagatga cgtgcgcctg gtcgccggcg aggtcatagg agaatccaaa gcgcggctgg
     1981 aaggagtcct gctgcggatc gcgattgttg cccgtgctga tgtagtcgtt gatgtcgatg
     2041 gccacattcg gatcggtgga tagacccagc gactgaccgt acgtgactcc cggcgagact
     2101 tccgtgttga gcgcatccag caggaattgc ggcgtctcga agtcgagata ggactcgttc
     2161 cactcgacgt cccagcgaat gcccaggttc agcgtgaggt gatcgttgac ggtccagtcg
     2221 tcctgcacga acaggcctaa ctgcttgtcg ttggtggtga cctcggagtc gaagcccgcc
     2281 agcggcagcg cgaacgcggt cttccatgga acggtggccg tgcccgccgg agtcacgtcg
     2341 taatagaaaa cggggttgcc gggaacggag tccgcggccg tgaggtcgac gtccttgtat
     2401 ttcacgcccg ccttgatggt gtggtcgccg gcgccccagg caagatccga gaacgtgatg
     2461 aagttgccga tcgcccaacc cttctggcct ttgttctggc cggcgcgcgg atcggcgccg
     2521 tccaccgcca gcacgttgcg gtcctggccg tcgaaccacg tgtacaccgc gccgttgacg
     2581 tcggagttgg tgatctgcgg cacgtagaac gcgtcttcgt aggtgagctg cagttcgttg
     2641 agccagctgt ccgcgctgtg tttccaggac aactcgaggc gagtgtcgtc attgttggtg
     2701 tcgacggtgg cgctgcgcgc gataccaacg cccgtacggt cgccagagct cacctcatcg
     2761 cggaccttcg cggagaactg caggctgtca cgctcggaca tcgcccagtc gagtttcgcg
     2821 aagaacaggt cttcttcgaa atcgatggag gtggggccga gttgggcgag cgcaccggca
     2881 ggtagctggg cgacgacgtc gctgggcgca aaggccggca gcacgctgac cggagtgacg
     2941 aagcgtttgc cttcataggt cacgaagaag tgaagtttgt cctcgatgat cgggccgccg
     3001 aacgcgacgc catattcctt gctctcggac tcggccttgc gccccgagtc gagttcggcc
     3061 ggagtcttgg cgcgaaaatt gtcggcggag taggtgccga aagcttcgcc gctgaacgta
     3121 ttggttcccg agcgcgagag cgccgtgatc gccgcgctcg agatctggtc gtattccgcc
     3181 ttgtagttcg acgtgatgac cttgtattcg ccgatggcga gctgcgggaa tggatttccc
     3241 tgcgtgtcac cggcctgtcc gctgacgccg gatcgcacgt agcctttctg acccacgccg
     3301 tcgatgtaga gattcacgcc gttgcggttc tgcgcgccgc cgctgatctt cgtgcggccg
     3361 ctgccgtcga cttcgaacac gacgccggga atggtgtcgg cgaactcgag gaagttgcgc
     3421 gtgatttgag gcaccgtctc gatctggtgt tccgacaccg tcgacccgac ctctgaagtg
     3481 cggacttcct ggaggcgccg tccctggacg gtgatttcct gcatcggggc atcaggcgct
     3541 tcgaccgcgc cgctcgtcgc gatcaggtcg agcgtcgcgg tcgacgccac gctcagcgtc
     3601 accgtagtcg cggttccggg tccggcatcg accgtgtacg tgcccggcgg aagtccggtc
     3661 agtgagtaaa tgccctcggc gtttgcctgg gtgcgacgcg ttgcaccggt ggcgacattc
     3721 ttcgcggtga tggcggtgtt ggccggcgcc ttgccgcgca gtgcggcgtc cgcggactgt
     3781 gcccaggtga tgacgggtgt ggcgagaacg gcggcgatcg ccgcgccaag aatggtacgg
     3841 ggaaaattgt tcatgaaagc ccctcgagtg ttcgtcgttc gcacggcggc aacactcaca
     3901 tcgcagtgcc gcgttgtgaa tagccgacat gcttgctatg cggcgattct cacactccgc
     3961 gcgcgcggaa tctcgatggt tccggtttcg atgtgtagga ctgatacggg gatgcagtgc
     4021 ggcggcgtcc gacagcatgc gactagttag gactaaagtc gcggccgcac tgcagcagtt
     4081 cagtttgtca gctcgaacgt tccggtgagg ccgccacggg cgctcgtgcc cagcgtgact
     4141 tcgtaagtgc cgctttcgag cgcccagcga tcctgtgaat tcatcgtcac gagactcttc
     4201 gcgggcaacg tgaactcgat gatcttcgat tcggcggccg ccagatgcac gcggcggtat
     4261 gcctgcagac gttgcccggt cacgccggac gccacgcgcg gccggatgta gagctgcggc
     4321 acttcgtcgc cggcgcggtc accgtggttg gtcaggcgta cgcggatcgt cagcgtatcc
     4381 gcggggccga tgcgtttcgg caactcgagc gcgctcagac tgaagcgcgt atacgacagc
     4441 ccgtagccga acgcgaacag cggatcgtcc gactcatcga gccagtgtgc cttgtagtcg
     4501 ccgcgctcgg ggatgttagg ccggccgttg ggcggatcgt aatggtggat cggcaactgg
     4561 ccgacgctgc gcggccaggt gacgggttgt ttgccacccg ggttgtagtc gccgaacagg
     4621 acgtcggcga gggcgttgcc gccttcaatg cccggaaaga atgaatgcac cagcgcgccg
     4681 gactgctcgg cgagtttcgg aaccgcgagc gcgcgtcccg tgatgagcac cacgactacg
     4741 ggtttgccgg tggcgacgag cgcatcgacg agcgcctgct ggctgccggg gagatcgagg
     4801 tacgcacgat tcttggcctc gccgctctgg gattcgcgct cgcccaccac cgcgacgatg
     4861 agatcgctgt tgtgcgcttc gctgaccgcg gcagagatgc gctgggcgtc gccgtcgacc
     4921 gccttgccct tgccgtcgat ctgcgcgcct tcggaataac tgaccgtgac cgcgggcttg
     4981 gccgacgctc cgcgctggcg gatgccggtc agcacgctga tgacgtcctt cgcctcacca
     5041 cgcgcatgcc acgggcccaa caggtcctcg cgcgcatcgg cgagcgggcc cgtgacgaga
     5101 attcgtctgg cgtgcagcgg cagcaggttc gcgtcgttct tgagcagcac aatggacttg
     5161 cgcgcgatct cgcgcgcggc cgcgcgatgc tccgcggaaa cgcgcacgcg cttctcgagg
     5221 tcttcgggaa cgtagggctt ttcgaacagg ccgagccggc gcttgaaggc gagcgcgcgc
     5281 aggacttcgc gatcgacctc cgcttcgcgc aagcgcccgg ctgccacggc gcgcgcgagc
     5341 tgcgcgtagt acacgccgtc ttccatgtcg acggtcatgg ttccttcgcg cagcgattgc
     5401 accgccgctt ccagatcgtc cgccgccgtg ccgtggttgc gcatctcggt gatgcccgcg
     5461 tagtcggaga cgagcacgcc gttgaaaccg agttcgccgc gcaggacgtc ctgcagcagc
     5521 ttgcgatgcg cggacatggg cacgccaccg ggaccggtcg tgaatgccgg catcaccgag
     5581 ggcgcgccag ccgcgacacc cgccgcgaat ggcggcagat ggaactcgcg gatgcttcgc
     5641 tcggtgagat cgacataggc gtagtccttg ccaccctgga cggcgccgta gcccaggtaa
     5701 tgcttgagac agctcgcgat gccgaaatcc acgggcgcag gctttcccgg caattgcgcg
     5761 agcgatccgt gaaagccgcg aatcgtggcg accgcgtaat cgctgctcac cagcggcgat
     5821 tcgccgaagg tttcgaccac gcggccccag cgcgggtcgg cgggatgatc gaccatgggc
     5881 gcgaacgtcc agcgctgacc gtgcgagtag ccttcgaggc cggagatgcc ggccacgacg
     5941 cgcgcatcg
//
LOCUS       DATDEU010000719         7121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_284915, whole genome shotgun sequence.
ACCESSION   DATDEU010000719 DATDEU010000000
VERSION     DATDEU010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284915"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VIV63_17115"
     CDS             <1..491
                     /locus_tag="VIV63_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126377.1"
                     /translation="GDNERARVAYDNGVLVMSGKGDSPSNCSPITCITGDQAYRCEVE
                     IEVEGAATGGVLFFYNKRMYCGLGFNDRQMVRHRTAIDATRAKPKGIDRRLFIRFENN
                     RHIVTFHTSPDGAAWTKYDVQMEVSGYHHNVAYDFLSLRPGLYASGDGKVRFRNFKYQ
                     AI"
     gene            574..1440
                     /locus_tag="VIV63_17120"
     CDS             574..1440
                     /locus_tag="VIV63_17120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY6126378.1"
                     /translation="MTTTYLPFLLLLTAVLGLWIHRTVWIAALAAAVLAGIFTGALHW
                     LAAIWIALLAALAIAYTRARDSASPNKRLWQTLAGLVFAIYALAMGLALLPGFHRIVL
                     VQPQVLSDGATPYGISVGFPKVVTGIVILGLINPLLCTSWRELGRVLARAFPVFVITA
                     IIGMTVVLAMGYSSFAPKWTALFLLFAPVNLFFTCLSEEAFFRGFIQHELSRIGSHRA
                     LAAGIALTFAAVLFGLAHFGGGWTYVLAAAVAGLGYGWAFMRTQRIEAAMAVHFGVNA
                     THFLLFTYPALA"
     gene            1437..2603
                     /locus_tag="VIV63_17125"
     CDS             1437..2603
                     /locus_tag="VIV63_17125"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY6126379.1"
                     /translation="MIRFVRWLSMFALVLAGAIAIAEAPIYAVVPGLFDQSKPDLGLR
                     AAPNLQTITIFKPQPGTDQFSNGVVLLPFKGQLYAQWQSSPKDEDSQDTWVAYSISSD
                     GSTWSTPIALARAGKAPQMHSSGGWWTDGKTLVAYINVWPTGFQSGQGGYTDYLLSSD
                     GLRWSSPRRVTGYDGKPLAGVIEQDPHLLPGGRLLTAFHLQPGLNVAPFHTDDPLGRR
                     GWRRGQMPTHPFEQNVTRELEPSLFHRGTCAVMVFRDQASTFRQLAAESCDAGETWST
                     PALTDMPDSPAKQSAGNLPDGTAYLVNAPRTDRLRIPLAITLSRDGKVFDRAWLLRGP
                     ADLQPRRFEGLYKRPGYHYPKSVIWNDHLYVGYATNKEDVELTRVPLARLGTAR"
     gene            complement(2604..3146)
                     /locus_tag="VIV63_17130"
     CDS             complement(2604..3146)
                     /locus_tag="VIV63_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126380.1"
                     /translation="MAGNRADWLTAIFLVAFVASGQTHAAKPQPAASKVSSDKTVATT
                     LTPAQTSKLAMLKAQHLKAKAALPDQQQIHLDAIAGIVGDRLFAALPSSDLPATATRL
                     VRESVPALSDVEASAVGAYALDAIASGVQRDPETQLAFNLQYLQLQSQMRALSREYEA
                     LGRIMQSKHDMVENSISNVR"
     gene            complement(3146..4477)
                     /gene="rimO"
                     /locus_tag="VIV63_17135"
     CDS             complement(3146..4477)
                     /gene="rimO"
                     /locus_tag="VIV63_17135"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY6126381.1"
                     /translation="MSRRQPKVGFVSLGCPKALVDSERIITQLRAEGYQVAPNYESAD
                     VVVVNTCGFIDSAVDESLDAIGEALEQNGKVIVTGCLGARPEKILERHPKVLKVTGPH
                     AYEDVLGAVHEHLPPKHDPFLDLVPPQGIRLTPRHYAYLKISEGCNNKCSFCIIPSMR
                     GLLVSRPIDEVLREGEKLARAGVRELLVISQDTSAYGVDLRYREAEWRGQKYETRFLD
                     LARGLGELGIWVRMHYVYPYPHVDDVIPLMAERKVLPYLDIPFQHGSPTVLKRMKRPG
                     MVEKTLGRIKAWREVCPDLTIRSTFIVGFPGETEAEFEELLAWLDEAQLDRVGAFKYS
                     PVEGAAANDVAPHVPPDIQEERLARFMQKSAGISAAKLAAKVGQRTTVLVDGVDGNVA
                     IARSMADAPEIDGVVRIAKGGKLPVGEFADVEITASSDHDLEARLVSQRKS"
     gene            4694..5281
                     /locus_tag="VIV63_17140"
     CDS             4694..5281
                     /locus_tag="VIV63_17140"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY6126382.1"
                     /translation="MTLVAAIHAAVLLAIMRSLGFGAPAEDPPPMITDIYKEVRPPED
                     RPPPPEPDVRFRTETQVMPRPDLEQVAFEQDIISLPPPEADFTERPPTGDNTPQPVIE
                     KARPDPRYPLSRPQYSARMIREGNQGNIDLEVYVLPNGRVGDARVVKSTGFEELDLAA
                     LAEAKRNWRLLPATRDGVAVAQWHRLRVTFKLNER"
     gene            complement(5291..5446)
                     /locus_tag="VIV63_17145"
     CDS             complement(5291..5446)
                     /locus_tag="VIV63_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126383.1"
                     /translation="MSEPEKKGSKWPWILVPIAAFSIFFGLRECQHRLPPAEHADAAA
                     PEAPAPQ"
     gene            5504..5944
                     /locus_tag="VIV63_17150"
     CDS             5504..5944
                     /locus_tag="VIV63_17150"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEY6126384.1"
                     /translation="MALRILDLAARLLLALGVGCLLLAGYLAWRTLSFSGEAVAVTGE
                     VVSYREIPDGDSTRYVPRIRFVTATGEIVTVGGQLAATSKRFEIGAKVPMVYDPREPM
                     HARVALFVDNWLGPTIAAIVGLVGIAGGLLVRRSVRRELAKVRA"
     gene            5941..6528
                     /locus_tag="VIV63_17155"
     CDS             5941..6528
                     /locus_tag="VIV63_17155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6126385.1"
                     /translation="MSTQFDKAYYQRFYLDPRTAVVSRAEMKARANLIAAYANHIGLP
                     VRRMLDAGCGIGLLKTPLTRAFPRAEYTGLEYSEYLCERFGWTQGSLAEFTADPFDLT
                     VCYDVLQYLDDRTAARAIANLGRLTRGVLYLSALTTKDWRENCDRTRTDRDVFLRDAA
                     WYSQRLRKDFKPSGVGFWVRRGAPLTTWEMETAAI"
     gene            6553..6741
                     /locus_tag="VIV63_17160"
     CDS             6553..6741
                     /locus_tag="VIV63_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126386.1"
                     /translation="MPDRLPIDATTGDVRHRDVRAEVFAAVPQQRASIGKRAFWRIVL
                     FVARFPAGLRLLKRIRGS"
     gene            6745..>7121
                     /locus_tag="VIV63_17165"
     CDS             6745..>7121
                     /locus_tag="VIV63_17165"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6126387.1"
                     /translation="MKNIAYLLGIASGLGLTLQIGMNSKLRTVLQNANSAAFVSFLVG
                     TVALVVLLVATRTSLPDRDTLASVPVWAWFGGFLGAFNVAVATVVASQLGTASLLGLA
                     LLGQLAMAMVADHFGWMGLPEHP"
BASE COUNT         1204 a         2388 c         2177 g         1352 t
ORIGIN      
        1 cgggagacaa cgagcgcgca cgcgtcgcat acgacaacgg cgtgctggtg atgagcggca
       61 aaggcgacag cccctctaac tgctcaccca tcacctgcat caccggcgac caggcctatc
      121 gctgcgaggt ggagatcgaa gtcgagggcg cggccacggg cggcgttctc ttcttctata
      181 acaagcgcat gtactgcggc ctcggcttca acgaccgcca gatggtgcgt caccgtaccg
      241 ccatcgacgc cacgcgcgcg aaaccgaagg gtatcgaccg gcgcctgttc atccgcttcg
      301 agaacaaccg tcacatcgtc accttccaca cctcgcccga cggcgccgcc tggaccaagt
      361 acgacgtgca gatggaagtg tccggctatc atcacaacgt cgcgtacgac ttcctgagcc
      421 tgcggcccgg cctctatgcc tcgggcgacg gaaaagttcg cttccggaac ttcaagtacc
      481 aggcgattta atggggacat ggatgtgtgg ggactgactt aagtctgtcc ccactctttt
      541 acctagcaaa aggggccgga cctcgacctc atcgtgacga caacctacct cccattcctg
      601 ctgctcctca cggccgtcct cggcttgtgg atccatcgca cggtctggat cgccgcgctc
      661 gccgccgcgg tcctcgccgg catcttcacg ggtgcgctcc actggctggc cgccatctgg
      721 atcgcgctgc tcgccgcgct ggccatcgcc tacacacgcg ctcgcgactc ggcttcgcct
      781 aacaagcgcc tctggcaaac gctcgccggc ctcgtcttcg cgatctacgc actcgccatg
      841 ggcctcgcgc tgctgccggg cttccatcgc atcgtgctcg tccagcccca ggtgctatcg
      901 gacggcgcca cgccctacgg catctctgtc ggtttcccga aggtcgtgac gggcatcgtg
      961 attctcggcc tcatcaaccc gttgctctgc acctcatggc gcgaactcgg gcgcgtgctg
     1021 gcgcgagcgt tccccgtgtt cgtgatcacc gcgatcatcg gcatgaccgt cgtgctcgcg
     1081 atgggttact ccagcttcgc gccaaagtgg accgcgctgt tcctgctctt cgcgccggtc
     1141 aatctcttct tcacgtgcct gtcggaggaa gccttcttcc gcggcttcat ccagcacgag
     1201 ctgtcgcgca tcggctcgca ccgcgcgctc gccgcgggaa tcgcactcac gttcgccgcc
     1261 gtgctgttcg gcctcgcgca cttcggcggc ggctggacct acgttctcgc cgccgccgtg
     1321 gcgggcctcg gttacggctg ggctttcatg cgcacgcagc gcatcgaagc cgcgatggcc
     1381 gtgcacttcg gtgtcaacgc cacgcacttc ctgttgttta cgtacccggc gctcgcttga
     1441 ttcgcttcgt gcgctggctc tcgatgttcg cgttggtact ggcaggtgct attgcgattg
     1501 ccgaggcccc gatttacgcc gtggtccctg gtctgttcga ccagagcaaa ccggatctag
     1561 gtctgcgagc ggcacccaac ctccagacca tcaccatctt caaaccacaa ccgggcaccg
     1621 accaattcag caacggcgtc gtgctgctgc ctttcaaggg ccagttgtac gcccagtggc
     1681 agagctcgcc gaaggacgag gattcgcagg acacctgggt cgcctacagc atcagcagcg
     1741 atggctcgac gtggtcgacg cccatcgcac tggcccgcgc gggcaaggcg ccgcagatgc
     1801 actcgagcgg cggctggtgg accgacggca aaacgctggt cgcgtacatc aacgtctggc
     1861 ccaccggctt tcaatccggt cagggcggct acaccgacta tctactgagc agcgacggtc
     1921 tgcgctggtc ttcacccaga cgggtgaccg gttacgacgg taaacctctc gccggtgtga
     1981 tcgaacagga cccgcatctg ctgcccggcg ggcggctcct gaccgccttc catctgcaac
     2041 ccggtctcaa cgtcgcaccg ttccacacgg acgatccgct gggccgacgc ggctggcgcc
     2101 ggggccagat gcccactcat cctttcgagc agaacgtgac ccgcgaactc gagcccagcc
     2161 tgttccaccg tggaacctgc gcggtgatgg tgttccgcga tcaggcctcc actttccgcc
     2221 agctcgctgc cgaaagctgc gatgccggag aaacctggtc tacgccggcg ctgaccgaca
     2281 tgcccgattc gcccgccaaa caaagcgcag ggaatcttcc ggatggcacg gcgtacctcg
     2341 tcaacgcgcc gcgaaccgat cgtctgcgta ttccgctggc catcaccttg agtcgcgacg
     2401 gcaaggtttt cgatcgcgcc tggttgttgc gcggacccgc cgatctgcag ccccggcggt
     2461 tcgaaggttt gtacaagcgg ccgggttatc actaccccaa gagcgtgatc tggaacgatc
     2521 acctctacgt gggttatgcg acgaacaagg aagacgtgga gctcacgcgg gtgccgctcg
     2581 cgcgactcgg aaccgcgcgc tgatcagcga acgttgctga tcgagttctc aaccatgtcg
     2641 tgtttcgact gcatgatcct gcccaaggct tcgtattccc ggctcaaggc gcgcatctgc
     2701 gactggagct gcagatactg caggttgaaa gccaactggg tttccgggtc ccgctgcacg
     2761 ccactggcga tcgcgtccag ggcataagcc cccacggcgg aagcctcgac gtcactgaga
     2821 gccggtaccg actcgcgcac gagccgcgtt gccgtggcgg gcaaatcgct gctcgggagc
     2881 gcggcaaaca acctgtcgcc cacgatcccg gcgattgcat cgagatgaat ctgctgttga
     2941 tccggcaacg ccgctttcgc cttcaggtgc tgcgctttca gcatggcgag tttgctggtt
     3001 tgcgccggcg tcagagtggt ggcgacagtc ttgtcgctcg acactttgct ggccgcgggc
     3061 tgtggcttgg ccgcgtgggt ctggccggac gcgacgaatg caaccaggaa aatcgcggtg
     3121 agccagtcag cgcggtttcc agccatcacg atttgcgctg cgaaacgagc ctggcttcga
     3181 ggtcgtgatc gctcgacgcc gtgatctcca cgtccgcgaa ctcgccgacc ggtagtttgc
     3241 cgcctttggc gatacgaacc acgccgtcga tctccggcgc atcggccatg ctgcgcgcga
     3301 tcgcgacgtt gccgtcgacg ccatcgacca gcaccgtcgt gcgctgcccg accttcgccg
     3361 ccagcttcgc ggcgctgatg cccgcggact tctgcatgaa tctcgcgaga cgttcttcct
     3421 ggatatccgg cggcacatgc ggcgccacgt cgttcgccgc ggcgccttcc accggcgaat
     3481 acttgaacgc gcccacgcga tcgagctgtg cctcgtcgag ccacgctaac aactcttcga
     3541 actccgcttc ggtctcgccc gggaagccga cgatgaacgt cgatcggatc gtcaggtccg
     3601 gacagacttc gcgccacgcc ttgatgcgac ccaaagtctt ctcgaccatg ccaggccgct
     3661 tcatgcgctt cagcaccgtg gggctgccgt gctggaacgg aatgtcgagg tagggcagaa
     3721 ccttgcgctc ggccatcagc gggatcacgt cgtccacgtg cggatacgga tacacgtagt
     3781 gcatgcgcac ccagatgccg agttcgccca gtccgcgcgc cagatcgagg aatcgcgttt
     3841 cgtatttctg cccgcgccat tcggcttcgc ggtagcggag atcgacgcca tacgccgacg
     3901 tgtcctgcga aatgaccagc aactctcgta cacccgcgcg tgccagcttc tcgccttcgc
     3961 gcagcacttc atcgatgggc cggctgacta acaaaccgcg catcgacggg atgatgcaga
     4021 agctgcactt gttgttgcag ccttcggaga tcttgagata cgcgtaatga cgcggtgtga
     4081 ggcggatgcc ctgcggcggc acgagatcca ggaaaggatc gtgcttcggc ggcagatgct
     4141 cgtgtaccgc gcccagcacg tcttcatagg cgtgcggtcc tgtgaccttc agcaccttcg
     4201 gatgacgctc gagaatcttc tccggccgtg cgccgagaca gccggtgacg atcaccttcc
     4261 cgttctgctc gagcgcttcg ccgatcgcat cgagcgattc atccacggcc gagtcgatga
     4321 agccgcacgt gttcaccacc acgacatccg cggattcgta gttgggcgca acctggtaac
     4381 cctcggcgcg aagctgggta atgatgcgtt cggaatccac cagggccttg gggcaaccca
     4441 agctgacgaa tccgactttt ggctggcggc ggctcatgag ggggcgcaat ggtacacgcg
     4501 ccggcagcct caggcgacgc ggcgctgaca tatgccctct ggagcggaaa acgccttact
     4561 tcgtgctctt acggcgtgtt tgctaacccc ggaccgttcg caggctcgtt caccctgcat
     4621 acaccctcac cgggtgagcg gaggccgaat gactactcac acagcggcgc gtcccctggg
     4681 cgcattaggt cgaatgactt tggtggcggc catccacgcc gcggtgttgc tcgccatcat
     4741 gcgaagcctc ggcttcggag ctcctgccga agatccgccg cccatgatca ccgacatcta
     4801 caaggaagtg cggccgcccg aagacagacc gccgccgccg gagcccgatg tgcgtttccg
     4861 gaccgaaacc caggtcatgc cgcggcccga tctcgagcag gtcgccttcg agcaggacat
     4921 catttcgctg ccgccgccgg aagccgattt caccgaacgc ccgcccaccg gcgacaacac
     4981 gccacagccc gtcatcgaaa aggcgcgccc cgatccgcgt tatccattga gccgcccgca
     5041 gtactcggcg cgcatgatcc gcgaaggcaa tcagggaaac atcgatctcg aggtctacgt
     5101 actgccgaac ggccgcgtgg gcgacgcacg cgtggtcaag agcacgggat tcgaagagct
     5161 ggatctcgcg gcgctggccg aggcgaagcg gaactggcgg ctgctgccgg cgacgcgcga
     5221 cggcgtagcc gttgcgcagt ggcatcggct gcgcgtgacc ttcaagctca acgagcgata
     5281 ggaggcgggc ttattgtggc gccggagctt ccggcgccgc agcgtccgca tgttcggcgg
     5341 gcggcagacg gtgctggcat tcgcgcaggc cgaagaaaat gctgaacgcg gcaatgggta
     5401 ccaggatcca tggccacttg gatcctttct tctccggctc gctcatcgtg ctccccacat
     5461 cgaatagact cgcgaatcta ttgtgattcc gtgaatcctg caaatggcgc ttcgaattct
     5521 cgatctcgca gcccgcctgc tgctcgcact cggcgtgggt tgtctcttgc tggccggtta
     5581 cctcgcgtgg cgaacgttga gtttctcggg tgaagccgtc gcggtcactg gcgaggtggt
     5641 tagctaccgc gaaattcccg atggcgactc gacgcgttac gtgccgcgca tccggttcgt
     5701 gaccgctacg ggtgaaatag tcaccgtggg tggacaactg gccgcgacgt cgaagcgttt
     5761 cgagatcggc gcgaaagtgc ccatggtcta cgacccgcgc gagcccatgc atgcgcgcgt
     5821 ggcgctgttc gtcgacaact ggttaggtcc gaccatcgcc gcgatcgtcg gcctggtcgg
     5881 tatcgccggt gggctgctgg tgcgccgctc cgtgcggcgt gaactcgcga aggtgcgcgc
     5941 gtgagcacgc agttcgacaa ggcctactac cagcgcttct atctggatcc acgcacagcc
     6001 gtggtctctc gcgccgaaat gaaagcgcgc gcgaacctca tcgcggccta cgcaaatcac
     6061 atcggcctgc cggtgcgccg catgctggac gcgggttgcg gtatcgggct tttgaagacg
     6121 ccgctcacac gggcttttcc gcgcgccgaa tacacgggcc tggagtacag cgaatatctt
     6181 tgtgagcgct tcggctggac gcagggctcg ctcgcggaat tcacggccga tccgttcgac
     6241 ctcacggtct gttacgacgt gctgcagtac ctcgacgatc gcacggcagc gcgcgccatc
     6301 gccaatctcg gccggctcac gcgcggcgtg ttgtacctct ccgcgttgac cacgaaggac
     6361 tggcgcgaaa actgcgatcg cacgcgcacc gatcgcgacg tcttcctgcg cgacgcggcg
     6421 tggtattcgc aaaggctccg gaaagacttc aaaccgagcg gcgtgggatt ctgggtccgg
     6481 cgtggcgcgc cgctgaccac gtgggaaatg gaaaccgccg caatctgacg gcgcaaggta
     6541 tccttgcgcg ccatgccaga tcgcctgccc atcgatgcca ccaccggcga cgtccgtcat
     6601 cgcgatgtgc gcgccgaagt gttcgcggcc gtgccgcagc agcgggcgtc catcggcaaa
     6661 cgcgcgttct ggcgcatcgt gctgttcgtg gcccggttcc cggcggggct caggctcctg
     6721 aaacgcatcc gaggtagcta gtccatgaaa aacatcgcct atcttcttgg catcgcttcg
     6781 ggcctgggtc tcacgctgca gatcggcatg aattcgaaac tgcgcacagt cctgcagaac
     6841 gccaactcgg cggcgttcgt gagcttcctg gtcggcaccg tggcgctcgt ggtgctgctc
     6901 gtcgccacac gcacgtcgct gcccgaccgt gacacgctgg cgtcggtgcc ggtgtgggcc
     6961 tggttcggtg gcttcctggg cgcgttcaac gttgccgtcg cgacggtagt tgcatctcag
     7021 ctcggcaccg ccagcctgct tggactcgca ttgctgggcc agctggccat ggcaatggtc
     7081 gccgaccact tcgggtggat gggcctgccc gaacatccga t
//
LOCUS       DATDEU010000720         8463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1307692, whole genome shotgun sequence.
ACCESSION   DATDEU010000720 DATDEU010000000
VERSION     DATDEU010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307692"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1292)
                     /locus_tag="VIV63_17170"
     CDS             complement(126..1292)
                     /locus_tag="VIV63_17170"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY6126388.1"
                     /translation="MKVPLLDLKPQYHALKPALDAALLRVAESQYFILGPEVKALEAA
                     TAAYSGCKFGIGISSGTDALLIALMALDIGAGDEVITSPYTFFATAGTIARAGARPVF
                     VDIDPGTFNISPQAIERFIATCEVRGGDLVNPATGGRVRALMPVHLYGQMADMTRIME
                     IARRHGLRVIEDAAQAIGSEDEQGRRACSIGDIGCLSFFPTKNLGAFGDAGACVTNDP
                     VLAAKLMKLRVHGMEPKYYHELIGGNFRLDEIQAAVLNVKLPHLDSWSEARQRNAAFY
                     DAAFARAGLGGKVLTPPPAAKGSRHIYNQYCIRVERRDELRAWLTKHEVGAEIYYPLA
                     LHMQQCFAYLGHRPADFPESLRATLESLALPIYPELTQAQLQFVVDTVAEFVRS"
     gene            complement(1308..2348)
                     /locus_tag="VIV63_17175"
     CDS             complement(1308..2348)
                     /locus_tag="VIV63_17175"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEY6126389.1"
                     /translation="MKILLTGAAGFIGSHVAQKLLARGDEVVGLDNLNDYYDPNLKRA
                     RLANLERQPKFRFVKMDLADKGGMKALFAREKFPRVIHLAAQAGVRYSLENPQAYIDS
                     NVTGTLNVLEGCRHNNAEHLVFASTSSVYGLNTNMPFTPHRGVDHPLSLYATTKRANE
                     LMAHNYAALFKLPVTGLRFFTVYGPWGRPDMALFLFTKNILEGKPIDVFNHGHHKRDF
                     TFVDDIAEGVVRACDRPATPNNDWSGDNPDPATSSAPFRIYNIGNNAPVDLSRYIEVL
                     EDCLGRKAVKNMLPLQPGDVPDTFADVEDLVRDVGYKPATPVEVGIRRFVDWYLEYYK
                     TNAPQQPKATIA"
     gene            complement(2345..3613)
                     /gene="tviB"
                     /locus_tag="VIV63_17180"
     CDS             complement(2345..3613)
                     /gene="tviB"
                     /locus_tag="VIV63_17180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HEY6126390.1"
                     /translation="MRGCRIGVVGLGYVGLPLAVEFGKHFDTVGFDIKADRIAELKKG
                     RDSTLECTTAELRAARKLKYSTSLKDLAKARVFIITVPTPIDKYNRPDLTPLERSSET
                     VGKVLKKGDVVVYESTVYPGCTEEICVPILEQRSGLKFNRDFYAGYSPERINPGDKEH
                     RLPSIKKVTSGSTPEVANFVDELYRTVVRAGTHKASSIRVAEAAKVIENTQRDVNIAL
                     INELALLFNRLGIDTEEVLLAAGSKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEIGFH
                     PEMILAGRRLNDSMASYVAGQVVKLMSSKRILVKGSRVLVLGLTFKENCPDLRNSKVA
                     DVVRELKNFGAKVDVYDPWIDRDEAEHEYGIRPVSRVRDGVYDAAVLAVGHKEFRDQG
                     IANIRKACKKTHVVYDIKYLFPAADVDGRL"
     gene            complement(3760..4497)
                     /gene="dnaQ"
                     /locus_tag="VIV63_17185"
     CDS             complement(3760..4497)
                     /gene="dnaQ"
                     /locus_tag="VIV63_17185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY6126391.1"
                     /translation="MRQIVLDVETTGLEAAAGHRIIEIGCVEILNRRPTGQKFHRYLN
                     PERDIDAGALAVHGIDSARLKDAPKFAEVVDELLVFIKGAELIIHNAPFDVGFLDAEF
                     ARLPGDVRTIISVCKVLDTLALARSLHPGQRNSLDALCKRYSVDNTKRELHGALLDAG
                     ILVDVYLAMTGGQSALALDMSGGRGQGTRHHGTTVPARAPGVALAVTAADPDELRQHE
                     AMLDLINKSSGGKAVWRAQVPPDALPS"
     gene            complement(4509..4946)
                     /gene="rnhA"
                     /locus_tag="VIV63_17190"
     CDS             complement(4509..4946)
                     /gene="rnhA"
                     /locus_tag="VIV63_17190"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY6126392.1"
                     /translation="MSGVDIYTDGACRGNPGPGGWGALLLFGEHEREISGAEANTTNN
                     RMELTAVIRALEALKRPVKGRIFTDSQYVRQGVLDWMPQWKARGWKTADKKPVKNQDL
                     WQMLDALVPMHQLEWHWVKGHSGNVGNERVDALANRAIDAMNG"
     gene            complement(4950..5603)
                     /locus_tag="VIV63_17195"
     CDS             complement(4950..5603)
                     /locus_tag="VIV63_17195"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6126393.1"
                     /translation="MSEALDDVFGWELLQLGTWGEKASLLAGCRTRRHAIIASGKFKS
                     TDADIGARLSQLPIANASVDGVVLPHTLEFETDPYAVVREADRVLAGEGQLVVLGFRP
                     FSLWGFRSRAISRGYPPGLKRMLGAGRIADWLELLGYDVTLTRNYLFAPPWGGSAPAP
                     GVPSALLRRGWIKPWPAGAYLIKARKRVYTLTPIRPKIRERPQTIGGLVKPTTRSSS"
     gene            6037..7548
                     /locus_tag="VIV63_17200"
     CDS             6037..7548
                     /locus_tag="VIV63_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY6126394.1"
                     /translation="MAAAELPTDFLAVAEVAVEAAEDAAGADNASRTIAGLTPEQYPD
                     VFDRIRAGFKLKDIDSRQIDVQLAFYANNPQYLERVFGRAVPYLHHIVQEIERRGMPL
                     ELALLPVVESAFEPYARSWATANGLWQFMPGTGERFGLKQDWWYDGRRDVMASTRAAL
                     DYLQYMHDEFFDDWLLAIAAYNCGEFRVQREVNKNRAAGKSVDFFSLSLPSETRAYVP
                     KLMAMRRLVANPEDYGIAFSPIPNEPYFARVETGGQLDLTLAAELAGITLDEVYELNP
                     GFHRWATDPAGPHFLLLPRDTADAFSKNLELLSPDERVRVSLHKVKSGESVASIAKKY
                     KTQTVVVRDMNSLENGAPLVVGSELRVPTAVVNLPAKVMLAAARVDGHGGRSGRRPAV
                     HVVRSGDSLWRIAKRNRMDVATLARLNGMGTGDTLRAGQKLVLNTRAPSSNRTASTGN
                     SRAVSYKVRSGDTLSRIAKVFGVTVSDLVNWNGISKQTTLRPGQKLTVNVRRH"
     gene            7613..8413
                     /locus_tag="VIV63_17205"
     CDS             7613..8413
                     /locus_tag="VIV63_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEY6126395.1"
                     /translation="MGFLSGKRALIVGVASDRSIAWGIAQAMHREGAELAFSYVNEKF
                     KERVQPLAESLGSKITMQLDVTNDAEIESAFATLKQSWDSLDIVVHAVAYAPREALAG
                     GFVESTSREAFRVAHDVSSYSLTALARAAKPMMAGRAGAILTLSYLGAVKSIPSYNVM
                     GLAKASLEANVRFLAADMGPNNIRVNGISAGPIKTLAAAGIAGFRKMLSRVADVAPLR
                     RNVTLEDVGNAAAFLCSDLANGVTGEILYVDGGFNTVGMSFPQEAGEG"
BASE COUNT         1436 a         2719 c         2742 g         1566 t
ORIGIN      
        1 agcgcgtcga gcaccacgat ccggtcttcc ggatgcgcgc gccgccagta gtggacgaaa
       61 ttcgagccga tgaatccggc gccgccggtg accaggaccc ttcgcaaccg aactcctcgc
      121 gcggactaac tgcggacgaa ctccgcgacc gtgtccacga cgaactggag ctgcgcctgc
      181 gtcagctccg ggtagatggg cagcgcgagc gattccagcg tggcgcgcag gctctcggga
      241 aaatccgcgg ggcggtgacc gaggtacgcg aaacactgct gcatgtgcag cgcgagcgga
      301 tagtagatct ccgcgcccac ctcgtgcttc gtgagccagg cccgcagttc gtcgcggcgt
      361 tcgacgcgga tgcagtactg gttgtagatg tgccgcgagc ctttcgccgc gggcggcggc
      421 gtgagcacct tgccgcccaa ccccgcgcgc gcgaaagccg cgtcgtaaaa agccgcattg
      481 cgctgacgcg cctcgctcca ggaatcgagg tgcggcagtt tcacgttgag cacggcggcc
      541 tggatttcat cgagccggaa attgccgccg atgagctcgt ggtaatactt cggttccatg
      601 ccatgcacgc gcagcttcat gagcttcgcc gccagcaccg gatcgttggt gacgcacgca
      661 ccggcgtcgc cgaacgcgcc caagttcttg gtcgggaaga acgacaggca gccgatgtcg
      721 ccgatggaac acgcgcgcct gccctgctcg tcctcggagc cgatggcttg tgctgcatct
      781 tcgatgacgc gcaatccatg acggcgcgcg atctccatga tgcgggtcat gtccgccatc
      841 tggccgtaca ggtgcacggg catgagcgcg cgcacgcggc cgccggtggc gggattgacg
      901 aggtcgccac cgcgtacttc gcaggtggcg atgaatcgct cgatcgcctg cggcgaaata
      961 ttgaaggtgc cggggtcgat gtcgacgaac accggacgcg cgcccgcgcg cgcgatcgtg
     1021 ccggcggtgg cgaagaacgt gtacgggctg gtgatgactt cgtcgcccgc gccgatgtcg
     1081 agcgccatga gcgcgatcag cagtgcgtcg gtgcccgatg aaatgccgat gccgaacttg
     1141 cagccggagt acgcggcggt cgcggcttcc agcgccttca cctcggggcc gaggatgaag
     1201 tactgggatt cggcgacgcg cagcagcgct gcgtcgagag ccggtttcag cgcgtggtat
     1261 tgcggcttga ggtcgaggag tgggactttc acggcccgca ctctacctca agcaatggtg
     1321 gccttgggtt gctgcggcgc gttggtcttg tagtactcga gataccagtc gacgaaccgg
     1381 cggatgccca cttcgaccgg cgtggccggc ttgtagccca cgtcgcgcac caggtcttcg
     1441 acgtccgcga acgtgtcggg tacatcgccc ggctgcagcg gcagcatgtt cttgacggcc
     1501 ttcctgccga gacagtcttc gagcacttcg atgtagcgcg agagatccac cggcgcgttg
     1561 ttgccgatgt tgtagatgcg gaacggcgcg ctgctggtgg ccggatccgg gttgtccccg
     1621 ctccagtcgt tgttaggcgt cgccgggcgg tcgcaggcgc ggaccacgcc ttcggcgatg
     1681 tcgtcgacga acgtgaagtc gcgtttgtgg tgcccgtgat tgaacacgtc gatgggcttg
     1741 ccttcgagga tgttcttggt gaacaggaac agcgccatgt cgggccggcc ccagggaccg
     1801 tacaccgtga agaaacgcag cccggtcacg ggcagcttga acagcgccgc gtagttgtgc
     1861 gccatgagtt cgttggcgcg cttggtcgtc gcgtacagcg acagcggatg atcgacgcca
     1921 cgatgcggcg tgaacggcat gttggtgttg agtccgtaga cggaactggt cgacgcgaac
     1981 acgaggtgtt cggcgttgtt gtggcggcag ccttcgagca cgttgagcgt gcccgtcacg
     2041 ttgctgtcga tgtaggcctg cggattctcg agcgagtaac gcacgccggc ctgtgccgcg
     2101 aggtggatga cgcgcggaaa cttctcgcga gcgaacagcg ccttcatgcc gcccttgtcg
     2161 gcgagatcca tcttcacgaa acggaatttc ggctgacgct cgaggttggc gagccgcgcg
     2221 cgcttcaggt tcggatcgta gtagtcattg aggttgtcga gacccactac ttcatcgcca
     2281 cgcgccagca gcttctgcgc cacgtgcgac ccgatgaacc cggcggcgcc agtcagcagg
     2341 atcttcatag gcgtccgtcg acgtccgcgg ccgggaacag gtatttgatg tcgtacacca
     2401 cgtgcgtctt cttgcatgcc ttgcggatgt tggcaattcc ctggtcgcgg aattccttgt
     2461 gacccacggc gagcaccgcg gcatcgtata cgccatcgcg tacacggctg accgggcgga
     2521 tgccgtattc gtgttcggct tcgtcgcgat cgatccacgg atcgtaaacg tcgaccttgg
     2581 cgccgaaatt cttgagctcg cgcaccacgt cggccacttt cgagttgcgc aggtccgggc
     2641 agttttcctt gaacgtgagg cccagcacca gcacgcgcga tcccttgacg aggatgcgct
     2701 tggacgacat gagcttgacg acctggccgg cgacgtacga cgccatgctg tcgttcaggc
     2761 ggcgtccggc gaggatcatc tcgggatgga atccgatctc ctgcgccttg tgcgtcaggt
     2821 agtaaggatc gacgccgatg caatggccgc ccacgagacc ggggcggaac ggcaggaagt
     2881 tccacttgct gcccgcggcc agcagcactt cttccgtgtc gattcccagg cggttgaaga
     2941 gcagggccag ttcgttgatg agcgcgatgt tgacgtcgcg ctgcgtgttc tcgatgacct
     3001 tggcggcttc ggcgacgcgg atgctggacg ccttgtgcgt gccggcgcgc accacggtgc
     3061 gatacagctc gtcgacgaag ttggcgactt caggcgtcga gcccgatgtg accttcttga
     3121 tgctcggcag gcgatgttcc ttgtcgcccg ggttgatgcg ctccgggctg tagccggcat
     3181 agaagtcgcg gttgaacttg agcccggaac gctgctcgag gatcggcacg cagatttcct
     3241 cggtgcagcc cgggtacacg gtggattcgt acaccaccac gtcgcccttc ttgagcacct
     3301 tgcccacggt ctcgctcgaa cgttcgagcg gcgtaaggtc cggacggttg tacttatcga
     3361 tgggcgtcgg caccgtgatg atgaagacgc gggccttggc cagatccttg aggctggtcg
     3421 agtacttgag cttgcgggcg gcgcgcagtt ccgccgtcgt gcactccagc gtgctgtcac
     3481 ggcccttctt cagctccgcg atgcggtccg ccttgatgtc gaaccccacc gtgtcgaagt
     3541 gtttgccgaa ctcgacagcc aggggcaagc ccacatagcc gagtccgacc acgccgatgc
     3601 gacagcctct catgctcaat tccatgattt ttccgttctc ttttaagcgc ttgggtgcaa
     3661 tcgcgcgcga tcttgccggt cctgggggga cgaaagcgag gccttctccc cctggatcag
     3721 tgatttctgt gcgctctatc gcgatccgtg aggcgaacct tatgacggga gggcatccgg
     3781 aggcacttgc gcccgccaga cagccttgcc gccgctcgac ttgttgatga ggtcgagcat
     3841 ggcttcgtgc tggcgcagct cgtcgggatc cgcggcggta accgccagcg cgacgcccgg
     3901 ggcgcgggca ggcacggtgg tgccgtgatg ccgcgtgccc tggccacgac cgccgctcat
     3961 gtcgagcgcg agggcgctct gcccgcccgt catggcgaga tacacgtcga ccagaattcc
     4021 cgcgtcgagc agcgcgccgt gcagttcgcg cttggtgttg tcgacgctgt accgcttgca
     4081 cagcgcatcg aggctgttgc gctgaccggg atgcaggctg cgggcgagcg ccagcgtgtc
     4141 gagtaccttg cagacgctga tgatggtgcg cacgtcgccc ggcaggcgcg cgaattcggc
     4201 gtcgaggaaa ccgacgtcga agggcgcgtt gtgaatgatg agttccgcgc ccttgatgaa
     4261 caccagcagc tcgtcgacga cctcggcgaa cttcggcgca tccttcagac gcgcggagtc
     4321 gatgccgtgc acggcgagcg cgccggcgtc gatgtcccgc tcgggattca ggtaacgatg
     4381 aaatttctgg ccggtcgggc ggcgattcag gatttccacg caaccgattt cgatgatgcg
     4441 gtggccggcg gcggcttcga ggccggtggt ttctacgtcg aggacgatct gtctcatggc
     4501 gcgcgagtct agccgttcat cgcatcgatg gcgcgattgg ccagcgcatc gacgcgttcg
     4561 ttgccgacat tgcccgagtg ccccttcacc cagtgccact cgagctggtg catcggcacc
     4621 agcgcatcga gcatctgcca gagatcctgg ttcttcacgg gcttcttgtc cgcggttttc
     4681 cagccgcgcg ccttccactg tggcatccag tcgagcacgc cctgccgcac gtattgcgag
     4741 tcggtgaaga tgcggccttt cacgggacgt ttcagcgctt cgagcgcgcg gatgaccgcg
     4801 gtgagttcca tgcggttgtt cgtggtgttc gcttccgcgc cggaaatttc gcgctcgtgc
     4861 tcgccgaaca gcagcaatgc cccccagcca cccggacccg ggttacctcg gcaggcaccg
     4921 tcggtgtaga tatcgacgcc gctcaagatc tacgagctcg agcgcgtcgt gggtttcacg
     4981 aggccaccga tggtctgcgg gcgttcccga atcttcggcc tgatgggcgt gagcgtgtac
     5041 acccgcttgc gcgccttgat gagataggcg cccgcgggcc agggcttgat ccatcctcgc
     5101 cgcagcaacg cggacgggac gccgggtgcc ggcgcgctgc cgccccaggg tggcgcgaat
     5161 agatagttgc gcgtcagcgt gacgtcgtag cccaggagtt ccagccagtc cgcgatacgc
     5221 ccggcgccca gcattcgctt gaggcccggc gggtaaccgc ggctgatcgc gcggctgcga
     5281 aacccccaga gactgaatgg acggaagcca agcaccacga gttggccctc gccggccagc
     5341 acccggtcgg cctcgcggac cacggcatag ggatcggttt cgaattcgag ggtatgcggc
     5401 aggaccacgc cgtcgaccga ggcgttggca atcggcaatt gtgaaagccg ggcgccgatg
     5461 tcggcatcgg tgcttttgaa ctttccgctg gcgatgatgg cgtgtcttcg cgtccggcaa
     5521 cccgccaaca gagaggcttt ttcaccccag gttccaagct gaagcaactc ccagccgaaa
     5581 acatcgtcca atgcttcgga catgagctcg gattcggcct gtagcacagc ccgccccagt
     5641 ccggaagcga gccaggaatc gacagcggtg gatgcagtgg cgtgcgtcac aaacggcgtg
     5701 aattagatca caaaaaaggt agcaaacttg tgaacttacg gcttaaatac cgcccatcga
     5761 agaagtaccg tcacatttgg taaagcggtc ctgcgacgtt ccccatgggg gtgtgaattg
     5821 actggtggat tcacggtgtg gcgccctctg gcgtccttgt tggttcttgc ggttgccctg
     5881 acgggctgcg caactaatcc tgaaattgcg aaggaagctc ctgtggccgt tccggcggtt
     5941 acggaaggta tccccgcgcc cgtcagccag gccgcgccgg cgcccgcccc gaccccggtg
     6001 gcagcacccc ggacccgggc cgccactccc gccctggtgg ctgccgccga actgcccacc
     6061 gacttcctgg ccgttgccga agtggccgtg gaggccgctg aagacgccgc cggggccgac
     6121 aacgcctcgc gcaccattgc cgggctgacg cccgagcaat atcccgacgt cttcgaccgt
     6181 atccgcgccg gcttcaagct caaggacatc gactcccgcc agatcgacgt ccagctcgcg
     6241 ttctacgcca acaatccgca gtacctcgag cgagtcttcg gccgcgccgt gccgtacctg
     6301 caccacatcg tccaggagat cgagcgccgc ggcatgccgc tcgaactcgc cctgctgccc
     6361 gtcgtcgaga gcgccttcga gccgtacgcg cgctcgtggg ccaccgcgaa cggcctgtgg
     6421 cagttcatgc ccggcaccgg cgaacgcttc ggactcaaac aggactggtg gtacgacggc
     6481 cgccgcgacg tcatggcctc gacgcgcgcc gcgctcgact acctgcagta catgcacgat
     6541 gaattcttcg acgactggtt gttagccatc gccgcctaca actgcggcga gttccgcgtg
     6601 cagcgcgaag tgaacaagaa tcgcgccgcc ggcaagtcgg tcgacttctt cagcttgagc
     6661 ctgccttcgg aaacgcgcgc ctatgttccg aagctgatgg ccatgcgccg cctcgtggcc
     6721 aatcccgagg actacggcat cgcgttcagc cccattccga atgaaccgta cttcgcgcgc
     6781 gtcgaaacgg gcggtcagct cgacctgacg ctggccgccg agcttgcggg catcactctc
     6841 gatgaagtgt acgaactgaa ccccggtttc caccggtggg ccaccgatcc ggccggcccg
     6901 cacttcctgc tgctgccgcg cgatacggcg gacgctttca gcaagaacct cgaactgttg
     6961 agccccgatg aacgcgtgcg cgtttcgctg cataaagtga agagcggcga gtcggtggcg
     7021 tccatcgcca agaaatacaa gacgcagacc gtcgtggttc gcgacatgaa ctctctcgag
     7081 aatggcgcgc ccctcgtggt cggcagcgaa ctgcgcgtgc ccacggcagt cgtcaatcta
     7141 cccgccaagg tgatgctcgc cgcggcgcgt gtcgatggtc acggcggccg ttctggccga
     7201 cgcccggcgg tacacgtggt gcgcagcggc gattcgctgt ggcgcatcgc caagcgcaac
     7261 cgcatggatg tcgcgacgct cgcacgactg aacggcatgg gtaccggcga cacgctgcgc
     7321 gccggccaga agctcgtgct gaatacgcgc gcaccgtcga gcaaccgcac ggcgtcgacc
     7381 ggcaattcgc gcgccgtttc ctacaaggtg cgcagcggcg atacgctgtc gcgcatcgcc
     7441 aaggtgttcg gggtgacggt ctccgacctc gtcaactgga acggcatcag caagcagacc
     7501 acgctgcgcc cgggccagaa actcacggtc aacgttcgcc gccactgatt tcgccccctc
     7561 gatgaggtga ggctggcaga atgccggcct tgccaactca aaggggatac acatgggttt
     7621 cctgagcgga aagcgcgcgc tcatcgtcgg cgtcgcgtcg gatcgttcca tcgcctgggg
     7681 cattgcgcag gccatgcatc gcgaaggcgc cgaactcgcg ttctcgtacg tcaacgagaa
     7741 attcaaggaa cgcgtgcagc cgctcgccga gtcactcggc tcgaagatca ccatgcagct
     7801 cgacgtcacc aacgacgccg agatcgaatc ggccttcgcc acgctcaagc agtcctggga
     7861 ttccctcgac atcgtcgtcc atgccgtcgc ctacgcgccc cgcgaggcgc tggccggggg
     7921 tttcgtcgag agcacctccc gcgaggcctt ccgggtcgcg cacgatgtgt cgagctacag
     7981 cctcaccgcc ctcgcccgtg ccgcgaagcc gatgatggcc ggacgagccg gcgccatcct
     8041 cacgctgtcc tatctgggcg ccgtgaaatc catcccgagc tacaacgtca tgggtctcgc
     8101 caaggccagc ctcgaagcca acgtgcggtt cctggccgcc gacatgggcc cgaacaacat
     8161 ccgcgtgaat ggcatttccg ccgggcctat caagacgctg gccgccgcgg gcatcgcggg
     8221 ctttcgcaag atgttgagcc gggtggccga tgtcgcgccg ctgcgccgca acgtcacgct
     8281 cgaagacgtg ggcaacgccg cggcgttcct ctgctcggat ctcgccaatg gcgtgaccgg
     8341 cgaaatcctc tacgtggacg gcggattcaa cacggtcggc atgagctttc cgcaggaagc
     8401 cggcgaaggc tgaaaaatgg ggacagtccc cattttctag gaattgggga ctgtccccat
     8461 ttc
//
LOCUS       DATDEU010000721         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_140297, whole genome shotgun sequence.
ACCESSION   DATDEU010000721 DATDEU010000000
VERSION     DATDEU010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140297"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VIV63_17210"
     CDS             <1..220
                     /locus_tag="VIV63_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126396.1"
                     /translation="HILPDLFKVVDEEGFEIVTLEEAQKDPAYDYDPDVADPRGGTLV
                     ELGMLAKNIPWPANIPRKPRDRLRTICE"
     gene            complement(225..1271)
                     /locus_tag="VIV63_17215"
     CDS             complement(225..1271)
                     /locus_tag="VIV63_17215"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6126397.1"
                     /translation="MSQKLGFGFIGAGAIAHFHARAVAAAQGGRLVGVVSRSRATAEK
                     FAAEHGIGFVSDDVDHLLAQPGLDAVCITTPSALHLVPALAAIRAGKHLMIEKPLDAT
                     VEGTDHILHEAAKAGVRVGSIFQARFGDAARALKAAVDAGRFGRMVLASCYVKWNRTA
                     EYYTGWKGRLSEDGGGAVINQAIHGVDLLQWFAGMPVEVFAWTTQRVHKIESEDTSVA
                     ALRFGSGAFGTIEASTALWPGFSRRIEICGENGSAVMEDDDITRWEFRVAKPEDEKIR
                     AARETSAKGSGAANPMAINFEGHLRQIQDFIDGIRDARPFFIEGAEARKAVALVRAIY
                     DSAASGEPVRPDGV"
     gene            complement(1303..>2133)
                     /locus_tag="VIV63_17220"
     CDS             complement(1303..>2133)
                     /locus_tag="VIV63_17220"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6126398.1"
                     /translation="DILRHTAGFHGEGSPEAVTAIYRQVASRDFNNTLPEVAARLAKV
                     PLSYQPGTQWKYSDAVDVQAYLVQKISGVPFDEYLKLHILRPLGMTSTRHTILPTDPD
                     RPQLAALYTRNEDGTFTRQTDEEAYGFNSAAWPYKPGSFGLVSTIDDYMKFARMLLGN
                     GKLGRARILKPETVRLMATDVMPKEVTEKSWLPGKGQVGFGIDFAVRIAPPKDAQESS
                     GAVGEFFWDGAASTLFWVDPKNDIAAVLFTQMRPFDKVKLHKTFRDAVYRNDPVALAH
                     "
BASE COUNT          364 a          714 c          664 g          391 t
ORIGIN      
        1 gcacatcctg cccgacctgt tcaaggtcgt cgatgaagag ggcttcgaga tcgtcacgct
       61 cgaggaagcg cagaaagacc cggcctacga ctacgacccg gacgtcgccg atccgcgcgg
      121 cggcactctg gtcgaactcg gcatgttggc caagaacatt ccctggcccg ccaacatccc
      181 gcgaaagccg cgcgaccggc ttcgaaccat ctgcgagtaa cccgtcagac tccgtcgggc
      241 ctcaccggct cgccggacgc cgccgaatcg tagatagccc gcaccagcgc cacggctttg
      301 cgtgcctcgg cgccttcgat gaagaacggc cgcgcatcgc ggatgccgtc gatgaaatcc
      361 tggatctgcc gcagatggcc ttcgaaattg atggccatgg gattcgcggc gcctgatccc
      421 ttggcactgg tctcacgcgc cgcacggatc ttctcgtctt cgggcttcgc gacacggaat
      481 tcccagcgcg tgatgtcatc gtcttccatg accgccgaac cgttctcgcc gcagatttcg
      541 atgcggcgcg agaaccccgg ccacaacgcc gtgctcgctt cgatggtgcc gaaagcgccg
      601 ctgccgaacc gcagcgcggc caccgacgtg tcttccgatt cgatcttgtg cacgcgctgc
      661 gtggtccacg caaacacttc cacgggcata ccggcaaacc actgcagcag atcgacaccg
      721 tgaatggcct ggttgatgac cgcgccgccg ccgtcctcgg agaggcgccc cttccagccg
      781 gtgtagtact cggcggtgcg attccacttc acgtagcagc tcgccagcac catgcggcca
      841 aaccgcccgg catcgaccgc cgccttgagc gcgcgcgcgg catcaccgaa gcgcgcctgg
      901 aaaatggaac cgacacgtac gccggccttg gccgcctcgt gcaggatgtg atccgtgcct
      961 tcgacggtgg cgtcgagcgg cttctcgatc atcaggtgtt tgcccgcacg gatcgccgcc
     1021 agcgcaggca cgaggtgcag cgccgatgga gtggtgatgc acacggcgtc gagcccgggt
     1081 tgcgcgagca ggtgatccac gtcgtccgat acgaagccga tgccgtgttc agccgcgaac
     1141 ttctcggcgg tcgcgcgtga tcggctgacc acgccgacca gccgtccgcc ctgggcggcg
     1201 gccactgcac gcgcgtggaa gtgcgcgatg gcgccggcgc cgatgaatcc gaaacccagt
     1261 ttttgactca ctccgtttcc tttttgacga gcctggcgtg aatcaatgcg cgagcgcgac
     1321 ggggtcgttg cgatacaccg cgtcgcggaa ggtcttgtgc agcttcactt tgtcgaacgg
     1381 gcgcatctgc gtgaacaaca ccgcggcgat gtcgttcttc ggatctaccc agaataaagt
     1441 gctggccgcg ccgtcccaga agaactcgcc cacggcgccc gaggattcct gcgcgtcctt
     1501 cggcggcgcg atgcgcactg cgaagtcgat gccgaaaccc acctgccctt tgccgggcaa
     1561 ccacgatttc tccgtgactt ccttgggcat gacatccgtt gccatgagcc tgaccgtttc
     1621 gggcttgagg atgcgcgccc ggcccaactt gccgttgccc agcagcatgc gcgcgaactt
     1681 catgtagtcg tcgatcgtgg acacgagacc gaagctgccg ggtttgtagg gccacgcggc
     1741 actgttgaag ccgtacgcct cctcgtcggt ctggcgcgtg aacgtgccgt cttcattgcg
     1801 cgtgtacaga gccgcgagct gcggccggtc cggatccgtc ggcagaatgg tgtgccgcgt
     1861 ggatgtcatg ccgagcggcc gcaggatgtg cagcttcagg tactcgtcga agggaacgcc
     1921 cgatattttc tgcaccaggt atgcctgcac atcgaccgcg tcgctgtact tccactgcgt
     1981 gcccggctgg taactcagcg gcactttcgc aagcctggcg gcaacctcgg gcagggtgtt
     2041 gttgaaatcg cgcgaggcga cctgccggta gatagccgtg accgcttcgg gagaaccttc
     2101 tccgtggaag ccggccgtgt ggcgcaggat gtc
//
LOCUS       DATDEU010000722         4094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1599495, whole genome shotgun sequence.
ACCESSION   DATDEU010000722 DATDEU010000000
VERSION     DATDEU010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599495"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VIV63_17225"
     CDS             <1..887
                     /locus_tag="VIV63_17225"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6126399.1"
                     /translation="DQHIPSLRANPRFTLAACASRNATVDGVPSFPDLEAMLAAVPDL
                     HCISICTPPQAHFDAALTALRAGKHVMLEKPPTATTRQIALLADESARRGRTLFQTWH
                     SRFAAGVDAAREFLRTRPLVSGKIIWKEDVHQWHPGQRWIWEPGGFGVFDPGINALSL
                     LTEILSQEVCVDAALLEFPENQQAPIAANVGLRTEDGARIDAEFDFRQKGEQSWDIEL
                     VTAGGKLTLAGGGAEVRIDGRAVTHDASMAGEYPRLYQRFASLCEAGKSEVDWRPFQL
                     VADAFLIGERRIVAPHVI"
     gene            891..2969
                     /locus_tag="VIV63_17230"
     CDS             891..2969
                     /locus_tag="VIV63_17230"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY6126400.1"
                     /translation="MAAPRWLWALLILPLCASAASAATRVDLNGDWAFRTEKGTEATA
                     SNPGGWSKAIPRNTRAVKVPHSWNREGADYDYLGTGWYFRRFDLPKLPVDAIAQLHFG
                     ATFYKARVWLNGVEIGAHEGGYSAYSFDVTPHLRDDNLLVVAVDNRPGMFTIPGFGAR
                     GAPDAWYDWWAYGGIVRDVWLNVHGPVRVGRQFIRSTLDGGKAAVTSRVTLVSRRAQR
                     VSVRATVTDPSGATVATRTQLATLNAGAQDVSFTAGLEKIERWDLDHPNLYRMNVQVL
                     DLDGKVIESASDVFGLREITVRERHLLINGQRVRLTGMTRHTDSPWDGLAESIGTLRH
                     DWEDMKSLNMTLTRPVHYAPGTRAADFADRRGILLIPEIPIWQASEEQLSNPQYLELA
                     KQQMRELIEQYGNHPSVFAWSVLNESAAGSPGGIRFFRAMRDYIKSLDPERYVTLADD
                     NLPKLTSAAESAANDADFLMMNQYFGAWHGPREALEPALDKVDRLFPDKMVIISEFGF
                     PGIFGKNATEADATRVSIIREQLPLLAKRDWIAGAILWCYQDYKSRRYFWPGQEQGYL
                     EHGIVDPNRQRKPSYFAWAELNAPAHVDARWTRNAGGEPAAFTFTLTPKGERELPYYS
                     LRDYRVSWQLIDADGKAFAEGGATVNPPGALSVARDVPARTNKGAFRLAIKLIRPDGS
                     QAMERVLGSQ"
     gene            2980..3747
                     /locus_tag="VIV63_17235"
     CDS             2980..3747
                     /locus_tag="VIV63_17235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6126401.1"
                     /translation="MTKIDDLNGKAVLITGASSGIGAALARAFAAQGAALALHYNAHE
                     DEAKALAAEIAASSPAPKLIRGDLSKRGEAKRVVNEAAAALGGLDVLINNAGGIGERR
                     PIADVDDALFDFVYDLNVRSVIAASQAAIPHFDKRGGGNIINVGSIAGIDGGGPGSSM
                     YSTAKAAVHNITRHLATDLAAKNIRVNTVAPGAIATPFHARTPQSRMDAMRDATAMGR
                     VGEAKDCVGAFLFLASDELSGYITGNIVHVNGGMYMP"
     gene            3744..>4094
                     /locus_tag="VIV63_17240"
     CDS             3744..>4094
                     /locus_tag="VIV63_17240"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008671 - 2-dehydro-3-deoxygalactonokinase
                     activity [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY6126402.1"
                     /translation="MKTPAFIAGDWGTSNLTLALCDEDGKAIERRKGPGAAESRGRFA
                     EVFDGLSADWLAAHGALPAVLCGMVGSAFGWREAPYLPAPADLYELADDAVSPRDGVS
                     IVRGMSCTNPLGAPD"
BASE COUNT          737 a         1354 c         1320 g          683 t
ORIGIN      
        1 gcgatcagca catcccgtcg ttgcgcgcga acccgcgctt cacgctcgcg gcgtgcgcga
       61 gccgcaacgc gacggtcgat ggggtaccga gtttcccgga tctcgaggcg atgctggccg
      121 cggtcccgga cctgcattgc atctccatct gcacgccacc gcaggcgcat ttcgacgccg
      181 cgctgaccgc gcttcgcgcc ggcaaacacg tgatgctcga aaagccgccg accgccacca
      241 cgcgccagat cgcgctgctc gccgatgaat ctgcgcgtcg cggccgcacg ctgttccaga
      301 cctggcactc gcgcttcgcc gcgggtgtcg acgcagcccg tgaattcctg cgaacgcgcc
      361 cgctggtgag cggcaagatc atctggaagg aagacgtgca ccaatggcac cccggccagc
      421 gctggatctg ggagccgggc ggcttcggcg tgttcgatcc gggcatcaac gcgttgtcgc
      481 tgctcaccga gatcctgtcg caggaagtct gcgtggacgc cgcgctgctc gagttcccgg
      541 agaaccagca ggcgccgatt gccgcgaacg tcggcttgcg tactgaagac ggcgcccgca
      601 tcgacgctga attcgatttc cgccagaaag gcgaacaaag ctgggatatc gaactcgtca
      661 ctgccggcgg caaactcacg ttggcgggcg gaggtgcgga agttcgcatc gacggcaggg
      721 ccgtgactca cgacgcgagc atggcgggcg aatatccgcg gctctatcag cgattcgcct
      781 cgctgtgcga ggcgggcaag tcggaagtgg attggcggcc gttccagctc gtcgccgacg
      841 ctttcctcat cggcgaacgc cgcatcgtcg caccacacgt gatctgaaac atggctgccc
      901 cgcgctggct ctgggctctg ctgatcctgc cgctgtgtgc ctccgcagcc agtgccgcaa
      961 cgcgcgtcga cctgaatggc gactgggcct tccgcactga aaagggtacg gaagccaccg
     1021 ccagcaatcc gggcgggtgg tcgaaagcca ttccacgcaa cacgcgcgcg gtgaaagtgc
     1081 cgcactcgtg gaatcgcgaa ggtgcggact acgactatct gggcaccggc tggtacttcc
     1141 gccgattcga tctgcccaaa ctacccgtgg acgcgatcgc gcagcttcac ttcggcgcca
     1201 ccttctacaa ggcgcgtgtg tggctcaacg gcgtggagat cggtgcgcac gagggcggct
     1261 acagcgccta ttcgttcgac gtgacgcccc acctgcgcga cgacaatctg ctcgtcgtcg
     1321 ccgtcgacaa ccggcctggc atgttcacca ttccgggctt cggcgcgcgc ggcgcaccgg
     1381 atgcctggta cgactggtgg gcgtacggcg gcatcgtgcg tgacgtctgg ctcaacgttc
     1441 acgggccggt gcgcgtcggg agacagttca tccgctcgac actcgacggc ggcaaagcgg
     1501 ccgtgacgag tcgcgtcacg ctcgtgagcc ggcgcgcgca acgtgtgtcg gtacgcgcga
     1561 ccgtcaccga tccgtcgggt gcgactgtcg cgacgcgcac gcaactcgcc accttgaacg
     1621 ccggcgcgca ggacgtgtcg ttcaccgcgg ggctcgagaa gatcgagcga tgggatctcg
     1681 atcatcccaa cctgtatcgc atgaacgtgc aggtgctgga cctcgacggg aaggtgatcg
     1741 aaagcgccag cgacgtcttc ggcctgcgcg aaatcacggt ccgcgaacgt catctgctga
     1801 tcaatggtca gcgcgtgcgc ctcaccggca tgacgcgcca cacggattcg ccatgggacg
     1861 gactcgccga atccattggc acccttcggc acgactggga agacatgaag agtctcaaca
     1921 tgactctgac ccggccggtg cactacgcgc ccggaacgcg cgcggcggat ttcgccgatc
     1981 ggcgcggcat cctgctgatt cccgagatcc ccatctggca ggcgagcgaa gaacagctct
     2041 cgaacccgca gtacctcgag ctcgcgaagc agcagatgcg cgagctcatc gagcagtacg
     2101 gcaatcatcc gagtgtgttc gcgtggagtg ttctcaacga gagtgcggcc ggctcgccgg
     2161 gcggaatccg cttcttccgc gcgatgcgtg attacatcaa gagcctcgat ccggaacgct
     2221 acgtcacgct ggccgatgac aatctgccga agctgacgag cgccgccgaa tccgcggcca
     2281 acgacgccga cttcctgatg atgaatcagt acttcggtgc gtggcacggg ccgcgtgaag
     2341 cgctcgaacc cgcgctcgac aaggtcgacc ggctgttccc ggacaagatg gtcatcattt
     2401 cggaattcgg ctttccgggc atcttcggca agaacgcgac cgaggccgac gccacgcgcg
     2461 tctcgatcat tcgcgaacag ctgccattgc tcgcgaagcg ggactggatc gcgggcgcca
     2521 tcctctggtg ttaccaggac tacaaatcgc ggcgctactt ctggcccggc caggaacagg
     2581 gatatctcga acacggcatc gtcgatccca atcgtcagcg caagccttcc tatttcgcgt
     2641 gggccgagct caatgcaccg gcgcacgtcg acgcgcgctg gacgcggaat gcgggcggcg
     2701 agcctgccgc cttcacgttc acgctgactc cgaaaggcga gcgtgagctg ccctactact
     2761 cgctgcgcga ctaccgggtg agctggcagc tcatcgatgc cgacggcaag gcgttcgcgg
     2821 aggggggagc caccgtgaat ccgccgggcg cactgtccgt cgcgcgcgac gtaccggcgc
     2881 gcaccaacaa gggcgcattc cggctcgcga tcaaactaat ccgtcccgat ggttcacagg
     2941 ccatggagcg cgtgctcggc tctcagtaac atggcccgca tgaccaaaat cgacgacttg
     3001 aatggcaaag cggtgctcat caccggcgca agctcaggga taggcgcggc gcttgctcgc
     3061 gcgttcgctg cacagggcgc tgcccttgca ctgcactaca acgcgcatga agacgaagca
     3121 aaggcgctgg ctgcggaaat tgccgcgtct tcgcccgcac cgaagctcat tcgcggcgac
     3181 ctttcgaagc gcggcgaagc gaaacgcgtg gtgaacgaag cggcggcggc gttgggcgga
     3241 ctcgacgtcc tcatcaacaa tgccggcggc attggcgagc ggcggcccat tgccgacgtc
     3301 gacgacgcgc tgttcgattt cgtctacgac ctcaacgtcc gttcggtgat cgccgcgtcg
     3361 caagctgcga tcccgcattt cgacaagcgc ggaggcggca acatcatcaa cgtgggttcg
     3421 atcgccggca tcgatggcgg aggccctggc tcgagcatgt attccacggc caaagccgcg
     3481 gttcacaaca tcacgcgtca tctcgccacc gatctcgccg cgaagaacat tcgcgtgaac
     3541 accgtcgctc ccggcgccat cgccacgccg tttcacgcgc gtacgccgca atcgcgcatg
     3601 gacgcgatgc gcgacgccac cgccatgggg cgcgtgggcg aagcgaaaga ctgcgtcggg
     3661 gcgttcctgt tcctcgcgtc ggacgagctg tcgggataca tcacaggcaa catcgtgcac
     3721 gtgaacggcg gcatgtacat gccatgaaga ctcccgcctt catcgccggt gactggggca
     3781 cgagcaatct gacgctcgcg ctttgcgacg aggacgggaa ggcgatcgaa cgccgcaaag
     3841 ggccgggtgc cgcggaatcc cgcggccggt tcgcggaagt attcgatggg ctgtcggcgg
     3901 actggctcgc cgcacatggt gcgctgcctg cggtcctgtg cggcatggtg gggtcggctt
     3961 ttggatggcg cgaggcgccc tatctaccag cgcccgccga tctttacgag ctcgccgatg
     4021 acgcggtgag cccccgcgac ggcgtgagta tcgtacgcgg catgagttgc acgaatcccc
     4081 tgggcgcgcc cgac
//
LOCUS       DATDEU010000723         3070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_430836, whole genome shotgun sequence.
ACCESSION   DATDEU010000723 DATDEU010000000
VERSION     DATDEU010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430836"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2177
                     /gene="glnE"
                     /locus_tag="VIV63_17245"
     CDS             <1..2177
                     /gene="glnE"
                     /locus_tag="VIV63_17245"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY6126403.1"
                     /translation="FLNDYLQTHGRDWERYAWIKARAVTGVAAYKLIQAESVRPFVFR
                     RYLDFGVFEALREMKGLIEKEVARRDLADHVKLGPGGIREIEFVVQAFQLIRGGQDRR
                     MQTPSLLAVLPQLAGGKLLPVRVAQELEAAYVFLRRLENRLQMLADAQVHTIPPDALT
                     RERLALAMGFDDWQACGTELDLHRARVTRAFQEVMFARNEAVPAEMPGVNGIAEAWVR
                     GADETQLAAALASRGYVETAQAAELLTEFRSNGMLRRLDAHGRARLDTLVPRLVVAIA
                     DQKGDTQVDVLRRILRVLEAIGSRSAYFALLNENAQVRRKLVELAAHGEFLAAQIASH
                     PLLLDELLDESAGSRPTPRAELEAEVNARLAHLAEDEPERQVEVLRQFQRAAIFRVAM
                     ADLTGSIPLMRVSDYLTEIAEIIVEQAMRLAWTQMTAQFGVPQTNRGDGPGRRAVNIC
                     AVGYGKLGGNELGYASDLDLVFLHDARGENAETDAAKPVDNQVFFIRFAQRVLHLLTM
                     HSAAGRLYEVDVRLRPSGKGGMLITRVGAFAEYQEKEAWTWEHQALLHARAVAGDAAL
                     RAEFERLRVELLMRCVRRDTLRAEVRNMRERMRKELSKARPGSGKFDIKQDAGGTADI
                     EFLAQYWALLHAGTHPPVVMFADTIRQLESVASANLVPQETVDVLTHAYRKYRERAHH
                     LSLEKAEAVVPVEDFALDRAAVSAIWNATMVTSDETVAASPV"
     gene            2187..2381
                     /locus_tag="VIV63_17250"
     CDS             2187..2381
                     /locus_tag="VIV63_17250"
                     /inference="COORDINATES: protein motif:HMM:NF021772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY6126404.1"
                     /translation="MSATKPLIQPNAQNEYRVDASDLPLACPMPEMYLWNSHPRVYLA
                     IEATGWAKCPYCGAEYSLTK"
     gene            complement(2486..>3070)
                     /locus_tag="VIV63_17255"
     CDS             complement(2486..>3070)
                     /locus_tag="VIV63_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY6126405.1"
                     /translation="SMMEAAHTALGGSHEFALILLGEASAYPHGSGKPQAVRDGEVVL
                     MDCGCSVEGYQSDISRTFVFGDATAEQRKVWQQMRDGQQIAIETARVGVPAGAVDDAV
                     RAQYVKWGYGPGYKLPGLSHRTGHGIGLDGHEPVNLVHGEATPLAPGMCFSDEPGIYL
                     PGKFGIRLEDCFHMGPEKPIWFSTPPPSLDKPFD"
BASE COUNT          541 a          966 c         1035 g          528 t
ORIGIN      
        1 atttcctcaa cgactacctg cagacgcatg gccgcgactg ggagcgctat gcgtggatca
       61 aggcgcgcgc cgtcaccggc gtcgcggctt acaaactgat ccaggccgag agcgtccggc
      121 cgttcgtttt ccgccgatac ctcgacttcg gagttttcga agcgctacgc gaaatgaagg
      181 ggctcatcga aaaggaggtt gcacgccgcg atctcgccga tcacgtgaag ctggggccgg
      241 gcggtattcg cgagatcgag ttcgtcgtgc aggcgttcca gctcatccgt ggggggcagg
      301 accggcgcat gcagacgccg tcgctgctgg cggtgctgcc gcaactggcc gggggcaagt
      361 tgctgcccgt gcgggtcgcc caggaactcg aagcggccta tgtgttcctg cggcgcctcg
      421 agaatcgcct gcagatgctg gccgacgcgc aggtgcacac cattccgccg gacgcgctca
      481 cgcgcgagcg ccttgcgctc gccatggggt tcgatgactg gcaggcctgc ggcaccgaac
      541 tggacctgca ccgcgcgcgg gtgacccggg ctttccagga agtcatgttc gcgcgcaacg
      601 aagcggtgcc tgccgaaatg ccgggcgtca acggcatcgc cgaagcctgg gtgagaggcg
      661 ccgacgaaac ccagctcgcc gcggcgctgg cgtcacgcgg ctatgtagag acggcgcagg
      721 cggcagaatt gctcaccgag tttcgttcga acggcatgtt gcgccggctc gatgcgcacg
      781 gccgggcgcg actggacacg ttggtacccc gcctggtggt ggccatcgcc gatcagaagg
      841 gcgacacgca ggtcgacgtg ctgcggcgca tcctgcgtgt gctcgaagcc atcggctcgc
      901 gttcggcgta tttcgcgctg ctcaacgaga acgcgcaggt acggcgcaaa ctcgtggagc
      961 tcgcggccca tggcgaattc ctggcggcgc agattgcgtc gcacccgctg ctgctcgacg
     1021 agttgctgga cgaaagcgcc ggttcgaggc cgacgccgcg cgcggagctc gaagcggaag
     1081 tgaacgcgcg gctcgcgcat ctggcggaag acgaacccga gcggcaggtg gaagtgctcc
     1141 ggcaatttca gcgcgccgcg atcttccgcg tcgccatggc cgatctcacc ggatcgattc
     1201 cgctgatgcg ggtcagcgac tacctgacgg aaatcgccga gatcatcgtc gagcaggcca
     1261 tgcggctcgc gtggacgcag atgacggcgc agttcggcgt gccgcagacc aatcgcggcg
     1321 acgggccggg gcggcgcgcc gtgaacatct gtgctgtcgg ctacgggaag ttaggcggca
     1381 acgaacttgg ttacgcctcg gacctggacc tcgtgttcct gcacgatgcg cgcggcgaga
     1441 atgccgagac cgacgcggcg aagcccgtgg acaaccaggt gttcttcatc cgcttcgcgc
     1501 agcgcgtgtt gcatctgctg accatgcact cggctgccgg aaggctgtac gaagtcgacg
     1561 tgcggctgcg gccatcgggc aagggcggca tgttgatcac gcgggtcggc gcgtttgccg
     1621 aataccagga gaaggaagcc tggacgtggg aacaccaggc gctgctgcat gcgcgtgccg
     1681 tggccggcga tgcggcgttg cgcgccgagt tcgaacggct gcgcgtcgaa ctgctcatgc
     1741 gctgcgtgcg gcgcgacacc ctgcgcgccg aagtgcgcaa catgcgtgaa cgcatgcgca
     1801 aagagctgtc gaaggcgagg ccgggatcgg gcaagttcga catcaagcag gatgccggcg
     1861 gcacggccga catcgagttc ctggcgcagt actgggcgct gctgcacgcg ggcacccatc
     1921 cgcccgtggt catgtttgcc gacaccatcc gccagctcga gtccgtggcc tcggcgaatc
     1981 tggtcccgca ggagacggtg gacgtgctga cgcacgcata cagaaaatat cgcgagcgcg
     2041 cgcaccacct gtcgctcgaa aaggcggaag cggttgtccc ggtggaggat tttgctttgg
     2101 atcgcgccgc cgtctcggcc atctggaacg cgaccatggt gacaagtgat gaaaccgtcg
     2161 cggcgtcgcc cgtataatcc gcgcccatgt cagccaccaa gccactgatc cagccgaacg
     2221 cgcagaacga ataccgggtg gacgccagcg atctgccgct cgcgtgcccg atgccggaga
     2281 tgtatctgtg gaactcgcac ccgcgcgtct atctcgcgat cgaagcgact ggttgggcga
     2341 agtgtccgta ctgcggcgcc gagtattccc tcacgaagtg aaatggggac attcctcatt
     2401 tccttggacg gagaaaaagg ggacagatct atttttcgca gcgaaaaata gatctgtccc
     2461 ctttttctcc gtcccctttt ctccgtcagt cgaagggttt gtcgagggac ggcggtgggg
     2521 ttgagaacca gatgggtttc tcggggccca tgtggaagca gtcttccagc ctgatgccga
     2581 acttcccggg tagatagatc cccggttcat ccgagaagca catccccggc gcgagcggtg
     2641 tcgcctcgcc atgcaccagg ttgacgggct catggccatc gaggccgatg ccgtggccgg
     2701 tgcggtgcga aaggcctggt agtttgtagc cggggccata accccatttc acgtactgcg
     2761 cgcgcacggc gtcatcgacg gcgcctgcgg gcacgcccac acgcgcagtc tcgatcgcga
     2821 tctgctggcc atcgcgcatc tgctgccaga ccttgcgctg ctcggcagtc gcgtcgccga
     2881 acacgaacgt gcgcgaaata tcggactggt acccctcgac gctgcaaccg cagtccatga
     2941 gcaccacttc gccatcgcga acggcttgcg gctttcccga accgtgcgga taggcggatg
     3001 cctcgcccag caggatcagc gcgaattcat gtgagccgcc gagcgcggtg tgggcggcct
     3061 ccatcatcga
//
LOCUS       DATDEU010000724         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_725177, whole genome shotgun sequence.
ACCESSION   DATDEU010000724 DATDEU010000000
VERSION     DATDEU010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725177"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VIV63_17260"
     CDS             <1..1155
                     /locus_tag="VIV63_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126406.1"
                     /translation="RTQIVGRSKLFASIPTDAQYSALNAAPDPLRDPVSFYAANLRQR
                     FGKEWEGPSAQVTSKRMRAWGLNTAYGAALNEALPAGSTLRQPYVFPLRGWQQSEGAI
                     MGLPDVYSDAFARRVDMEAAQQLGPRKSDPWMIGYFIGNEPPWPARESQLVDLVLAGP
                     ASSMQQRFKSELAKGDTPAIRKALVHAAFTRYLQIVNAAVKRSDPNHLNLGIRFGGTP
                     PDEVIALARGFDVYSMNKYRWAPPKDFIDRVYAIQKLPILIGEFHFGVPERGLAPGLV
                     QAMNQTERGVAYSYYVERAAEHPAIIGTHWYLWIDQPATGRRDGENYNIGWIDVTDRP
                     YPELVAAAKATHAKLDDIHSGRLAATTRKPKASEFGTPEDSIQLGIPAIQ"
     gene            complement(1335..>2284)
                     /locus_tag="VIV63_17265"
     CDS             complement(1335..>2284)
                     /locus_tag="VIV63_17265"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6126407.1"
                     /translation="TVADLAAHRSEWVEPVSTNYRGYDVWELPPNTQGVAALQMLNIL
                     EAYDLKSMGFGSADYLHVFVEAKKLAFEDRARYYVDPDFAKIPLKTLLSKDYAAKRRA
                     LIKLDRAAREYPVDAKALEQGDTIYMTVADSAGNMVSLIQSNYRGMGSGMTPTGCGFI
                     LQDRGELFSLEDGHASVYAPGKRPFHTIIPAFITRDGKPWLSFGLMGGAMQPQGHVQI
                     VVNLVDFGMNLQEAGDAPRARHDGSSEPTGEVMKDGGEVVLEVGALPATVKALEARGH
                     KVTVANDGDFGGYQAIMRNAEGVYFGASESRKDGAAQGY"
BASE COUNT          395 a          780 c          726 g          383 t
ORIGIN      
        1 cgaacgcaga tcgtcggccg ctcgaagttg tttgcgagca ttcccaccga tgcgcagtat
       61 tcggcgctga acgccgcgcc ggatcccctg cgcgatccag tgtcgttcta tgcggccaat
      121 ctccggcagc gcttcggcaa ggaatgggaa ggcccgagcg cgcaggtgac ctcgaaacgc
      181 atgcgcgcct gggggctcaa cacggcctat ggcgcggcgc tcaatgaggc actgccggcg
      241 ggctccacac tgcgccagcc gtatgtgttt ccgctccggg gctggcagca gagcgaaggc
      301 gcgatcatgg gacttcccga cgtgtattcc gacgccttcg cgcggcgcgt ggacatggag
      361 gccgcgcagc aactcgggcc gcgcaagtcc gatccgtgga tgatcggcta tttcatcggc
      421 aacgagcccc cgtggcccgc gcgcgagagc cagctcgtcg atctcgtgct ggccggcccc
      481 gcgagctcga tgcagcagcg tttcaagagc gagctcgcga agggcgacac cccggccatc
      541 cgcaaggcgc tcgtccacgc cgcgttcacg cgctacctgc aaatcgtgaa cgccgcggtc
      601 aagcgctccg accctaacca cctgaatctc ggcatccggt tcggcggcac tccgcccgac
      661 gaggtcatcg cgctggcgcg cggcttcgac gtgtacagca tgaacaagta ccgctgggcg
      721 ccgccgaagg atttcatcga ccgcgtgtac gccattcaga agctgccgat tctcatcggc
      781 gagttccact tcggcgtgcc ggagcgaggt ctcgcgcccg gcctcgtgca ggccatgaac
      841 cagaccgaac gtggcgtcgc gtattcctac tatgtcgagc gcgccgccga gcatcccgcc
      901 atcatcggca cgcactggta cctgtggata gaccagcccg cgaccgggcg gcgcgacggc
      961 gagaactaca acatcggctg gatcgacgtc accgaccgcc cctatccgga actggtcgcg
     1021 gccgcgaagg ccacgcacgc caaactcgac gacatccatt cgggacgact cgcggcgacc
     1081 acgcgcaagc cgaaggcatc ggagttcgga acgccggagg attccatcca gctggggatt
     1141 cccgcgattc agtgatgctt cgtggcgaac acctcgcctc ttcgttcagt gctggcgccg
     1201 atggaacgga acccccctga actcacgccg agctcccgca atcggtaaac agggctcgcg
     1261 cggaccgcac caccggcgcc aacggtggtg agtagcggcg ggttcgacaa tggttccggg
     1321 aacggcgccc gaagctaata accctgcgcc gcgccgtcct tgcgcgactc ggaagcgccg
     1381 aagtacacgc cctcggcatt tctcatgatg gcctggtagc cgccaaagtc gccgtcgttg
     1441 gccaccgtga ccttgtggcc ccgtgcctcg agcgccttca cggtggccgg cagggccccc
     1501 acttcgagca ccacctcgcc accatccttc atgacctcac ccgtcggttc gctcgaaccg
     1561 tcgtgccgcg cgcgcggtgc atcgccggcc tcctgcaggt tcatgccgaa atcgacgagg
     1621 ttgacgacga tctgcacgtg gccttgcggc tgcatggcgc cgcccatgag cccgaacgaa
     1681 agccagggct tgccgtccct ggtgatgaac gcgggaatga tggtgtggaa cggccgcttg
     1741 cccggcgcat agacgctggc gtgcccgtcc tcgagcgaga acagctcgcc gcggtcctgc
     1801 aggatgaacc cgcagcccgt gggcgtcatg cccgagccca tgccgcggta gttgctctgg
     1861 atcagcgaga ccatgttgcc cgccgagtcg gcgaccgtca tgtagatggt gtcgccctgt
     1921 tcgagcgctt tcgcgtcgac ggggtactcg cgcgcggcgc ggtcaagctt gatcagggcg
     1981 cggcgcttcg ccgcgtaatc cttcgagagc agcgtcttga gaggaatctt cgcgaagtcc
     2041 ggatccacgt agtaacgcgc gcggtcttcg aacgcgagct tcttggcctc gacgaacacg
     2101 tgcaggtagt cggcgctgcc gaatcccatg gatttgaggt cgtaggcctc gaggatgttg
     2161 agcatctgca acgccgccac gccctgcgtg ttcggcggca actcccatac atcgtagcct
     2221 cggtagttag tggacaccgg ctcgacccat tcggaacggt gggcggcaag atcggccacg
     2281 gtca
//
LOCUS       DATDEU010000725         4122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1164285, whole genome shotgun sequence.
ACCESSION   DATDEU010000725 DATDEU010000000
VERSION     DATDEU010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164285"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VIV63_17270"
     CDS             complement(<1..882)
                     /locus_tag="VIV63_17270"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY6126408.1"
                     /translation="MSLQSLWRAIRQRWLTLLFTSTLLFSAIAAYVFTLKPAYTAEAV
                     VLLAPVTEELGDAPTTARLLATTDPFFIRSETAIIGSDELSREVIGTLDLTHQAEFLP
                     QPGLLERLGVRPAPPTSAHPYLSAEELTLDQVVRGYQDRLSVFNDGRSKTVQIDFTAG
                     NPRVAASIANAHAEAYLHAQANRRGATRQKSLEWLKQEVDARAHEARDADARVQQYQL
                     ENGIVTSQDSTMVEQRLSQLSTQLVDARRQLSTQASLLAEIRAVRGGADPTNAAALMR
                     NDALSDLLRSRVQAESVV"
     gene            complement(904..2142)
                     /locus_tag="VIV63_17275"
     CDS             complement(904..2142)
                     /locus_tag="VIV63_17275"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6126409.1"
                     /translation="MREAFLSAARLHRHWLVAALFLSAAAQAQSLAPRALFIDDRGTE
                     LFDTEGAEGGIRAGSFDVRAAAGLSFGMDSNVYALPEAGDADRVATGEALIRAVNESP
                     TRDMLGMAFVRARRFDEARDQDATEFGVLGRYDGWIGSQDRIVTGISAEHGIESRNDI
                     ETPTTLALSPYDDIRGSFSYAHLFNRFQLESRVDARRLQYDAASQEYRDRSQYRAELR
                     GAYQMRSDMSWVVTGYFNRDDFDDPTPNTLSAGTAGALVGLHVDVNELLELEIGAGYF
                     NREYDDLDDPLDGFSFRGSLTWHPTRLTSVRAQVLRTDAPTHVEGAFGKVRNDALLRI
                     THEYSRNLVLHAGMRFIADDFETIDRLDTSWLAELGANWAFGRHSVLRFTYDYGTRDH
                     DDAGRSFDRHVASLAYIMRL"
     gene            complement(2124..2684)
                     /locus_tag="VIV63_17280"
     CDS             complement(2124..2684)
                     /locus_tag="VIV63_17280"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY6126410.1"
                     /translation="MKSLFLAVAFTLLTLAGCASPSGRLPDLPNDQSGAVYLLGPGDS
                     LRITVFGEADLSGTFKISDNGALVMPLVGQVSAAGLSVPELQKRLVTQLNVKAVKSPD
                     VTIQIEEYRPFFILGEVKNPGSYPYVPEMTVLTAVAIAGGFTFRASEDEVSVTRKRNG
                     AASESRATRASRILPGDVVYVFERHF"
     gene            complement(2681..3892)
                     /locus_tag="VIV63_17285"
     CDS             complement(2681..3892)
                     /locus_tag="VIV63_17285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6126411.1"
                     /translation="MYDRRVALIYDKLDVYGGGERVLEQQIAMYPQSDVCVSVDNLKP
                     EMRGFLQGKEVITTFAQNLPLVRHYQRHFLLLLMFAIEQLDLSDYDLVLSGSASIAKG
                     VITGPNQLHISYVHSPMRFAWDMQHQYLGEAKLDKGFRSMLARYMLHRARGWDLRSSN
                     GVDHFIANSQFIARRIWKVYRREATVIYPPVDVDSFSLRTNKEPFYLTASRMVPYKKI
                     TAIVEAFRALPERKLVVVGDGPEMKRIKTLAGPNVEILGYQPSSVLADLMQRAKCFIF
                     AAEEDFGITPVEAQACGTPVLAYGRGGALETIRGLDHDKPTGAFFDRQDAQSIADAVL
                     HFERNADRMAPLDCWQNAQRFSTQIFHENYGGFVERCWDAFRAAPAMQSGLDRQLALQ
                     EAARGFPGVAA"
     gene            complement(3900..>4122)
                     /locus_tag="VIV63_17290"
     CDS             complement(3900..>4122)
                     /locus_tag="VIV63_17290"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY6126412.1"
                     /translation="YRREIPSYMVRHKVKPGITGWAQVNGYRGGDDIESMRKRIELDL
                     AYLRHWSLWLDLRILFKTVTVVLTDRHAF"
BASE COUNT          709 a         1276 c         1411 g          726 t
ORIGIN      
        1 caccacggac tccgcctgca cgcggcttcg cagcaggtcc gacaacgcgt cgttgcgcat
       61 cagcgcggcg gcattggtcg ggtcggcgcc cccccgcacc gcgcggatct cggccagcag
      121 cgacgcctgg gtagatagct ggcgccgcgc gtcgacgagc tgcgtcgaga gttgcgacag
      181 ccgctgttcc accatcgtgg aatcctgcga ggtgacgatg ccgttctcga gctggtactg
      241 ctgcacgcgc gcatcggcgt cgcgggcttc gtgcgcgcgc gcgtccactt cctgtttgag
      301 ccattcgagc gatttctgcc gcgtcgcgcc gcgccggttg gcctgcgcgt gcaggtacgc
      361 ctcggcatga gcgttggcaa tcgaggccgc gacgcgcggg ttgcccgccg tgaaatcgat
      421 ctgcaccgtc ttgctgcggc cgtcgttgaa caccgagagg cgatcctggt agccgcgcac
      481 cacctggtcg agcgtcagtt cttcggccga cagatacgga tgagcgctgg tgggcggtgc
      541 agggcggacg ccgagccgct cgagcaggcc cggctgcggc aggaactcgg cctgatgcgt
      601 gagatccagc gtgccaatga cctcgcggct cagctcgtcg ctgccgatga tcgccgtttc
      661 gctgcggatg aagaacggat cggtggtcgc gagcagccgt gcggtggtgg gcgcgtcgcc
      721 cagttcttcg gtgaccggcg ccagcagcac gaccgcttcg gcggtgtacg cgggcttcaa
      781 cgtgaacacg tacgcggcaa tggccgaaaa cagcagcgtc gaggtgaaca gcagcgtgag
      841 ccagcgctgc cggatcgcgc gccacagact ctgcagactc attgccgaag tttcttccac
      901 tggttatagc ctcatgatgt aagcgaggct ggcgacgtgg cgatcgaagc tgcgccccgc
      961 atcgtcgtga tcgcgcgtgc cgtagtcgta ggtgaaacgc aggaccgagt gccggccgaa
     1021 agcccaattg gcgcccagct ccgcgagcca ggatgtatcg aggcgatcga tggtttcgaa
     1081 atcgtcggcg atgaatcgca tgcccgcgtg cagcacgagg ttgcggctgt attcatgggt
     1141 gatgcgcagc agcgcgtcgt tgcgaacctt gccgaaagcg ccttcgacgt gtgtcggcgc
     1201 gtcggtgcgc agcacctggg cgcgaaccga cgtcagccgc gtgggatgcc acgtgagcga
     1261 cccgcgaaac gaaaagccgt cgagcggatc atcgaggtcg tcgtactcgc ggttgaaata
     1321 gccggcgccg atctcgagtt cgagcagttc gttcacgtcg acgtgcaaac ccacgagcgc
     1381 accggcggtg ccggccgaga gtgtgttcgg cgtcggatcg tcgaagtcgt cgcgattgaa
     1441 ataccccgtg accacccagg acatgtcgga tcgcatttga taggcgccgc gaagttcggc
     1501 gcgatattgc gagcggtcac ggtattcctg ggaggccgcg tcgtattgca gccggcgggc
     1561 atcgacgcgg ctctcgagct gaaagcggtt gaacaggtgt gcgtacgaaa aggaaccgcg
     1621 aatgtcgtcg taggggctca gcgccagtgt tgtcggcgtt tcgatgtcgt tgcgcgattc
     1681 gatgccgtgt tcggcgctga ttccggtcac gatgcggtcc tgcgacccta tccacccgtc
     1741 gtagcgtccg agtacgccga actccgtggc gtcctggtcc cgcgcttcat cgaagcggcg
     1801 cgcccggacg aaggccatgc ccagcatgtc gcgcgtcgga gattcgttga ccgcgcgtat
     1861 caatgcctcg cccgttgcca cgcggtcggc gtcaccggcc tcgggcagcg cgtagacatt
     1921 ggagtccatg ccgaaggaca ggcccgcggc ggcgcgcaca tcgaaggagc cggcgcggat
     1981 gccaccttcg gcgccctcgg tgtcgaagag ttcggtgccg cggtcgtcga tgaacagcgc
     2041 gcgcggcgcg agtgactgcg cctgcgccgc ggcggacaga aacagcgccg cgaccagcca
     2101 atggcgatgg aggcgcgcgg cgctcagaaa tgcctctcga atacgtagac gacatcgccc
     2161 ggcaggatgc gcgaggcgcg cgtggcgcga gactcgctcg ccgcgccatt ccgtttgcgc
     2221 gtcaccgaca cctcgtcttc cgaggcgcga aaggtgaacc cgccggcgat ggcgacggcc
     2281 gtgagcaccg tcatttccgg aacgtagggg taagaaccgg gattcttcac ctcgcccagg
     2341 atgaagaacg gccggtattc ctcgatctgg atggtgacgt ccggcgactt caccgccttc
     2401 acgttgagtt gggtcaccag gcgcttctgc agttcgggca ccgacaaccc ggccgcggaa
     2461 acctgcccga cgagcggcat caccagcgcg ccgttatcgg aaatcttgaa cgtacccgac
     2521 agatccgctt cgccgaacac cgtgatgcgc agcgagtcgc cgggacccaa tagatagacg
     2581 gcgccgctct ggtcgttggg cagatcgggc aggcgtcccg agggactggc gcatcccgcg
     2641 agcgtgagga gcgtgaacgc aacggcgaga aacaatgact tcatgcggcg actccgggga
     2701 atccgcgggc cgcttcctgc agcgcgagct gacggtcaag gccgctctgc atggcgggtg
     2761 cggcgcggaa ggcgtcccag cagcgttcga cgaaacctcc gtagttctcg tggaagatct
     2821 gcgtggagaa ccgctgcgcg ttctgccagc agtcgagcgg ggccatgcga tccgcgttgc
     2881 gttcgaagtg caggaccgca tcggcgatgc tctgggcgtc ctgccggtcg aagaatgcgc
     2941 cggtgggctt gtcatgatcg agcccgcgaa tggtttcgag cgcgccgccg cggccgtacg
     3001 ccagtaccgg agtgccgcag gcctgggctt ccaccggcgt gatgccgaag tcctcctcgg
     3061 ccgcgaagat gaagcacttg gcgcgttgca tgagatcggc gagcacgctc gagggctggt
     3121 aaccgaggat ctccacgttg ggcccggcga gcgtcttgat gcgtttcatc tccggcccgt
     3181 cgccgacgac cacgagtttg cgttcgggaa gggcccggaa tgcttcgacg atggccgtga
     3241 tcttcttgta cggcaccatg cgcgaggcgg tgagatagaa cggctccttg ttggtccgca
     3301 gcgagaagct gtcgacgtcc accggcggat agatgaccgt cgcttcacgg cggtacacct
     3361 tccagatgcg gcgcgcgatg aactgcgagt tggcgatgaa gtgatcgacg ccgttgctgc
     3421 tgcgcagatc ccagccgcgc gcccggtgca acatgtagcg cgcgagcatg ctgcgaaagc
     3481 ccttgtcgag cttcgcttcg ccgagatact ggtgctgcat gtcccaggcg aagcgcatgg
     3541 gtgagtgcac ataggagatg tgcagctggt tgggcccggt gatgacgccc ttggcgatgg
     3601 acgcgctgcc cgacagcacc aggtcgtagt cggacaggtc gagctgctcg atggcgaaca
     3661 tcagcagcag caggaagtgg cgctggtagt gacggaccag cggcaggttc tgcgcgaacg
     3721 tggtgatgac ctctttgccc tggaggaacc cgcgcatctc cggcttcagg ttgtccaccg
     3781 atacacagac atcggattgc ggatacatcg cgatctgctg ttcgagcacg cgctcgccgc
     3841 ctccgtagac atccagcttg tcgtagatca gtgctactcg gcggtcgtac atgggctcct
     3901 cagaaggcgt ggcgatccgt cagcacgacg gtgacggtct tgaaaaggat ccggaggtcg
     3961 agccacagcg accaatggcg caggtaggcg aggtcgagct cgatgcgttt gcgcatggac
     4021 tcgatgtcgt cgccgccgcg atacccattg acctgcgccc atcctgtgat gccgggcttc
     4081 accttgtgcc ggaccatgta gctcggaatc tcgcggcgat ag
//
LOCUS       DATDEU010000726         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_725643, whole genome shotgun sequence.
ACCESSION   DATDEU010000726 DATDEU010000000
VERSION     DATDEU010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725643"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..737)
                     /locus_tag="VIV63_17295"
     CDS             complement(105..737)
                     /locus_tag="VIV63_17295"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY6126413.1"
                     /translation="MNLSAITLLVLTGCGGIGSTGPLPPVAEAGDNAPPIISGHAAAA
                     VVVGRAYNFAPLASDANGDRLTFGIRNRPSWASFDSATGRLSGTPNAAHTGEYANIVI
                     EVSDGHTTVGLTPFSIRVAAVGIGTALLQWMPPTHREDGTPLNNLAGYRVYYGEAPTN
                     FTEVVSLTNAGLTSYLVENLATGTWYFAVTAVDAAGVESPLSNSAFKTIS"
     gene            1082..>2006
                     /locus_tag="VIV63_17300"
     CDS             1082..>2006
                     /locus_tag="VIV63_17300"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126414.1"
                     /translation="MKIKFLNHYLNLPIAILALIETLLIIAAPMVVELLLRGRSSEDI
                     AWSVFLPRVVTFAFAVGVSLTAMGLYNSRQRARTIGLVLRAFVAHLLALGLVVIVSYF
                     APLLRPSPGQLFAWGALSFFFISVIRVVAGVLLDEEAFKRRVLVYGAGQRARSLRQLR
                     RRADQRGFHAVGYMPAPGEETLVEQDVVLAQGVGRLRELAKELNVDEIVIAMDDRRRE
                     FPLRELLDCRLNGIEVIDLVSFLERETGRVHLDVLNPSWIIFADGFRRDAMRQATKRL
                     FDMVASLALVLVTWPFMLFTMLAIVFEDGLRA"
BASE COUNT          316 a          618 c          653 g          419 t
ORIGIN      
        1 aggcctagga cactgccctt tcacggcggc gacaggggtt cgaatcccct tggggacgcc
       61 atctttcgcc gaagcgcacg tcagcgctgc ttcaagcccg atctctagct gatcgtcttg
      121 aatgcgctgt tcgacagcgg actctctacg cccgcggcgt ccaccgccgt cacggcgaaa
      181 taccaggtgc cggtcgccag gttctccacg agataggacg tgagccccgc gttcgtgagc
      241 gaaaccactt cggtgaagtt ggtgggcgcc tcgccgtaat acacgcgata gcccgccaga
      301 ttgttgaggg gcgtgccgtc ctcgcgatgc gtgggcggca tccactgcag cagcgcagtg
      361 ccgattccga ccgcagcgac gcgtatcgaa aatggcgtca accccaccgt ggtatggccg
      421 tcggacactt cgatgacgat gttcgcgtat tcgccggtgt gcgcggcgtt cggcgttccg
      481 ctgagtcgtc ccgtggccga gtcgaagctc gcccagctcg ggcgattgcg gattccgaag
      541 gtcagccggt cgccgttcgc atcggaggcg agcggcgcga agttataggc ccggcccacg
      601 acgaccgcgg cggctgcgtg gcctgaaatg atgggcggag cgttgtcgcc agcttccgcg
      661 accggaggca ggggcccggt actgccgatg ccaccgcagc cggtgagcac caaaagtgtg
      721 atggcgctca gattcacggc aatgcgctgg ccgtgcgtcc taaaaatcga tctcgttgta
      781 ggcatcatcg ccactccgct gtggctggag gccggctgac gattggccgc ctggcgcccc
      841 cacccgaact ggtggcgaga atatgttgcg tagtgtcttg catcaatata actaagcgtc
      901 tgttttcgga gacgtttttg ttaggggaga gtcatggttg cagctacaaa tccgagcttc
      961 ggcgcggagt tgcggatgct tgttgactca gttgctgact gccgacagtt cgccggcctt
     1021 tctcattaca aagtgagtaa gagaatttcg acgatgggtt cctgaggttt cgccctgtcc
     1081 gatgaagatc aagtttctca atcactacct caatcttccg atcgcgatcc tcgcgttgat
     1141 cgaaaccctg ttgatcatcg cggcgcccat ggtggtcgaa ctactgcttc gcggccgttc
     1201 gtccgaagac atcgcgtggt cggtgttcct gccgagggtc gtcacgttcg cgttcgccgt
     1261 cggcgtgtcg ctcactgcga tggggctcta caactcgcgc cagcgtgcgc gaacgatagg
     1321 cctggtactg cgcgccttcg tcgcgcacct gctggcgctc gggctcgtcg tcatcgtgtc
     1381 gtacttcgcg ccgttgctgc ggccgtcgcc ggggcagttg tttgcctggg gcgcgctgtc
     1441 gttcttcttc atcagcgtga ttcgcgtggt cgccggtgtc ctgctggacg aggaagcgtt
     1501 caagcgccgc gtgctcgtct atggcgccgg ccagcgcgcg cgcagcctgc gccagttgcg
     1561 ccgccgcgcc gatcaacgtg gatttcatgc cgtcggctac atgccggcgc ccggcgaaga
     1621 aacgctggtc gagcaggacg tggtgctggc gcagggcgtg ggccggctgc gcgagctcgc
     1681 caaggaactc aacgtcgacg agatcgtgat cgccatggac gatcggcgcc gcgaattccc
     1741 gttgcgcgag ctgctcgact gccggttgaa cggcatcgag gtcatcgacc tcgtgagttt
     1801 cctggaacgc gagaccggcc gcgtgcatct cgacgtgctg aatccgagct ggatcatctt
     1861 cgcggacggc ttccggcgcg acgcgatgcg ccaggccacc aagcgtctgt tcgacatggt
     1921 ggcttcgctg gcactggtgc tcgtgacctg gccgttcatg ctgttcacca tgctcgcgat
     1981 cgtgttcgaa gacgggctgc gcgcgc
//
LOCUS       DATDEU010000727         4753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1455570, whole genome shotgun sequence.
ACCESSION   DATDEU010000727 DATDEU010000000
VERSION     DATDEU010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455570"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VIV63_17305"
     CDS             <1..241
                     /locus_tag="VIV63_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY6126415.1"
                     /translation="IVCIVTLWNGVPLVVTPPPHIDLKVVETDPGLKGDTATGGQKVA
                     KLETGASVRVPLFINEGELLRIDTRTGEYVSRAKS"
     gene            complement(242..733)
                     /locus_tag="VIV63_17310"
     CDS             complement(242..733)
                     /locus_tag="VIV63_17310"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY6126416.1"
                     /translation="MSHASQSYDFSNAEAAYRELARDLEALLTGERDLIANAANMAAL
                     IYDALPGLNWAGFYLYKSGELVLGPFQGKPACVHIALGKGVCGTAAARRETVVVEDVH
                     AFPGHIACDSASNSEIVIPLVRGQELLGVLDLDSPLHARFGAADARGLEALTKIFLDS
                     LGD"
     gene            complement(733..1704)
                     /gene="epmA"
                     /locus_tag="VIV63_17315"
     CDS             complement(733..1704)
                     /gene="epmA"
                     /locus_tag="VIV63_17315"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEY6126417.1"
                     /translation="MNDASPWRPTATPGALRQRAQALRTTREFFHARQVMEVETPALI
                     NAPVSDVNLGSVRAQMSGSDAQLFLHTSPEYAMKRLLAAGSGDIFQICHVYRGAERGR
                     QHNPEFTMLEWYRLGFSLEQLMREVADLTRELLGQLELPIEFVTYRDAVRQHAGFDPL
                     DADIAELQHAAQSLGLGAAHAAKAGRDELLDLIVGAQVGPALGSATLTFLHRYPGSQA
                     ALARLDASDPRVALRFELYHRGIELANGYHELSSAAEQRMRFAADQMARASRGLPTFE
                     LDAHLLAALDAGLPDCAGVALGFDRVLMLAMNAASIDEVLAFPTERA"
     gene            complement(1708..2589)
                     /gene="asd"
                     /locus_tag="VIV63_17320"
     CDS             complement(1708..2589)
                     /gene="asd"
                     /locus_tag="VIV63_17320"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEY6126418.1"
                     /translation="MSDTPGAGGRAFVALQHLLPQHGISRLVLAATRSRSPGFKNALI
                     RLFVRGFNPDMTDAVETDPTAYPSFNEFFTRALRVGTRPVEADPQAIVSPVDGTVSEA
                     GDLTADRLLQAKGHEYSLRALLAGNTAWERTFAGGTFATIYLAPYNYHRIHMPLAGEL
                     REAFYVPGRLFSVNRTTAQLVPGLFSRNERVFCGFDSGGMPWANILVGALNVGSMATV
                     WHGDVTPRKVREVTALPVSGVLSPTTLGKGDEMARFNMGSTVILLFPRGAVRWDGKLK
                     AGNTLRMGERIGTLVER"
     gene            complement(2586..3212)
                     /locus_tag="VIV63_17325"
     CDS             complement(2586..3212)
                     /locus_tag="VIV63_17325"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY6126419.1"
                     /translation="MKTRSWIYIVALAAAGGIAGLYVGRMLTRPAIPSLEAGTSYPRP
                     KVLAEFDLVDTRGNKTSPATLSGHPSLVFFGFTYCPDVCPTTLAVLSSVQKQVGMPGL
                     KVVLISVDPERDTPEQLGKYISSFGGDLIGLTGPSPEIVKSLKTFGVAASRVDLPGGG
                     YTMDHSATIFALDKAARIVAVFTPPFNAAALTRDVERLEPVLSLGAGS"
     gene            3211..4098
                     /gene="prmB"
                     /locus_tag="VIV63_17330"
     CDS             3211..4098
                     /gene="prmB"
                     /locus_tag="VIV63_17330"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY6126420.1"
                     /translation="MARLFHINVGDLIASATRRLKRARVYFGHGTDNARDEAVALVLH
                     VMKLPHDAPVRVLTRRVSAAQRERFETLLKRRIADRVPLPYLTNEAWFAGLPFYVDER
                     VLIPRSAIAELIERRFAPWIDAARVKNVLDMGTGSGCIALACAKAFPRARVDAVDVDA
                     PALEVAAINRRRLRLTRRVEFIESDHFTGLSGRSYDIIVSNPPYVGEREMRGLPREYG
                     HEPRRALESGRDGLDSVATILHQARKFLRPQGLLVVEVGNTERILAKAFPKLPFTWLD
                     FERGGGGVFLLTREQLEKA"
     gene            4104..>4753
                     /gene="aroC"
                     /locus_tag="VIV63_17335"
     CDS             4104..>4753
                     /gene="aroC"
                     /locus_tag="VIV63_17335"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY6126421.1"
                     /translation="MSGNSIGRLFTVTTFGESHGPALGCIVDGCPPGLELSEADLQGD
                     VDRRRTGTSQFVSQRREGDAVKILSGVFEGKTTGTAIGILIENTDARSRDYEKIKDRF
                     RPGHADYTYQQKYGFRDYRGGGRSSARETVMRVAAGAIARKYLHARLGVSISGYLSQI
                     GSLTIDPVNPDAAYENPFFCPDPSRVQELEDLMWKIRGDGDSLGAKVTVVARGVPPG"
BASE COUNT          842 a         1504 c         1611 g          796 t
ORIGIN      
        1 catcgtctgc atcgtcacat tgtggaatgg cgtgccgctg gtcgtgaccc cgccgccgca
       61 catcgacctc aaggtcgtcg agaccgaccc gggcctcaag ggcgacaccg ccaccggtgg
      121 ccagaaggtt gccaagctcg agaccggcgc ctcggttcgc gtgccgctgt tcatcaacga
      181 aggcgagctg ttgcgcatcg acacgcgcac cggcgagtac gtctcgcgcg cgaagagcta
      241 gttagtcgcc gagcgagtcc aggaatatct tcgtcagcgc ctcgagcccg cgcgcgtcgg
      301 cggcgccgaa gcgcgcatgc aacgggctgt cgagatcgag cacgccgagc agttcctggc
      361 cgcgcacgag cggaatcacg atctcggaat tcgacgccga atcgcaggcg atgtgcccgg
      421 ggaatgcgtg tacgtcttcg acgaccacgg tttcgcggcg cgccgccgcc gtgccgcaaa
      481 cgcccttgcc cagggcgata tgcacgcagg cgggtttgcc ctggaagggc cccagcacca
      541 gttcgccact cttatataag tagaagccgg cccagttgag gccgggcagg gcgtcataga
      601 taagcgctgc catgttggcg gcattggcga tcaggtcgcg ttcgcctgtg agcaaggcct
      661 caagatcgcg ggcgagctcg cgatacgcgg cttccgcatt gctgaagtcg taagactggc
      721 tggcgtggct catcaggcgc gctccgtggg aaaggcgaga acttcatcga tgctcgcggc
      781 gttcatcgcg agcatgagca cgcggtcgaa cccgagcgcc acgcccgcgc aatcgggcag
      841 cccggcgtcg agcgccgcga gcagatgcgc atcgagttcg aaggtgggca ggccgcgtga
      901 ggcgcgcgcc atctgatccg cggcgaatcg catgcgctgt tctgccgcgc tcgagagctc
      961 gtgataaccg ttggcgagtt cgataccccg atggtagagc tcgaatcgca atgccacgcg
     1021 cgggtcgctg gcgtcgagcc gcgcgagcgc ggcctgcgag cccggataac gatgcaggaa
     1081 cgtgagggtg gccgagccca gcgccgggcc tacctgcgca ccgacgatga gatcgagcaa
     1141 ctcgtcgcgt ccggctttgg cggcgtgcgc cgcgccgagt cccagcgatt gcgcggcgtg
     1201 ctgcagttcg gcgatgtccg cgtccagcgg gtcgaagcct gcgtgctgcc gtaccgcgtc
     1261 gcgataggtc acgaactcga tcggtagctc aagctgaccc aggagttcgc gcgtgaggtc
     1321 ggcgacttcg cgcatcaatt gctcgagcga gaagccgagg cgataccact cgagcattgt
     1381 gaattcggga ttgtgctgcc ggccgcgctc ggcgccgcga tagacatggc agatctggaa
     1441 gatgtcaccg ctgccggcag ccagcaggcg cttcatcgcg tattcggggg acgtgtgcag
     1501 gaacaactgc gcgtcgcttc cggacatctg cgcgcgcacg ctgccgagat tcacgtcgga
     1561 caccggcgca ttgatcaggg ccggcgtctc gacttccatc acctggcgcg cgtggaaaaa
     1621 ctcccgagtg gtgcgcaggg cctgcgcgcg ctggcgcaat gccccgggag ttgcggtcgg
     1681 gcgccagggg ctcgcgtcat tcatctttca ccgctcgacg agagtgccga tgcgctcgcc
     1741 catgcgcagc gtattgccgg ccttgagttt gccgtcccaa cgaaccgcgc cgcgcggaaa
     1801 cagcaggatc acggtcgagc ccatgttgaa gcgggccatt tcgtcgccct tgccgagagt
     1861 cgtgggcgag agcacgccgc tcacgggtaa cgcggtgact tcgcgaacct tgcgcggcgt
     1921 gacgtcgcca tgccacacgg tcgccatgct tccgacgttc agcgcgccca ctaggatgtt
     1981 cgcccacggc atgccgcccg aatcgaagcc gcagaacacg cgctcattgc gcgagaacag
     2041 gccgggcacg agttgcgccg tggtccggtt cacgctgaac agccttcctg gaacgtagaa
     2101 cgcttcgcgc aattcaccgg cgagcggcat gtggatgcgg tgatagttgt acggcgccag
     2161 gtagatagtc gcgaaagtgc cgccggcgaa cgtgcgttcc caggcggtgt tgccggcgag
     2221 cagggcgcgc agcgaatact cgtggccctt ggcctgcaac aaccggtcgg cggtcagatc
     2281 gccggcttcg ctcaccgtgc catcgacggg cgagacgatc gcctggggat ccgcttccac
     2341 gggccgcgtg ccgacgcgca gcgcgcgcgt gaagaattca ttgaagctcg gataggcggt
     2401 cggatcggtc tccaccgcgt cggtcatgtc cggattgaaa ccgcgcacga acaggcggat
     2461 cagcgcgttc ttgaagcccg gcgaacgcga acgcgtcgcg gcgagcacga gccgggagat
     2521 gccgtgttgg ggcaacaggt gctggagcgc gacgaacgcg cggccgccag cgccgggagt
     2581 gtcgctcatg agcccgctcc cagggacagg accggttcga ggcgctcgac atcgcgcgtc
     2641 agcgccgcgg cgttgaacgg cggcgtgaag accgcgacga tgcgggccgc cttgtcgagc
     2701 gcgaatatcg tcgccgaatg gtccatggtg tagccgccgc cgggaagatc gacgcggctc
     2761 gcggccacgc caaaggtttt cagggacttc acaatctcgg gcgaaggccc ggtgaggccg
     2821 ataagatcgc cgccgaatga cgagatgtac ttgccgagtt gttcaggtgt gtcgcgctcg
     2881 gggtcgacgc tgatgagcac caccttgagg cccggcatgc ccacctgctt ctgcacgctc
     2941 gagagcaccg cgagcgtcgt ggggcacaca tcggggcaat acgtgaagcc gaagaacacc
     3001 agcgaaggat gccccgagag cgttgccggt gaggttttgt ttccccgggt atcgacgagg
     3061 tcgaattccg cgaggacctt gggccgggga tacgaagtgc ccgcttccag cgaaggaatg
     3121 gccggccgcg tcagcatgcg gcctacatac agaccggcga tgccgccggc cgcggcaagc
     3181 gcaacgatat aaatccagga acgagtcttc atggcgcgat tattccacat caacgtgggt
     3241 gacctgattg caagcgcaac gcggcgattg aagcgtgcgc gcgtgtattt cgggcatggc
     3301 accgacaatg cgcgcgacga agccgtggcg ctggtgctgc acgtcatgaa gctgccgcac
     3361 gacgcgcccg tccgggtgct gacccgccgc gtaagcgccg cgcaacgtga gcgcttcgag
     3421 acgctgctga aacggcgtat cgccgaccgg gtgccgctgc cctatctcac gaacgaggcc
     3481 tggttcgcgg gactgccgtt ctacgtcgac gaacgcgtgc tgattccgcg ctcggccatc
     3541 gccgagctca tcgagcggcg ctttgcgccc tggatcgacg ccgcccgcgt gaagaacgtg
     3601 ctcgacatgg gcacgggatc cggttgcatc gcgctcgcgt gtgcgaaggc ttttccacgc
     3661 gctcgcgtgg acgcggtcga cgtggacgca ccggcgctcg aagtcgcggc catcaatcgc
     3721 cgcaggttgc gtctaacacg acgggtggag ttcatcgaaa gcgaccattt cacggggctt
     3781 tcgggcaggt cctacgatat catcgtgagc aatccgccct atgtgggaga gcgcgagatg
     3841 cgcggcctgc cgcgcgagta cggtcacgaa ccgcgacgcg ctctcgaatc aggtcgcgat
     3901 ggtctcgact cggtcgcgac catcctgcat caggcgcgca agtttttgcg gccgcagggg
     3961 ctgctggtgg tcgaagtcgg caacaccgag cgaatcctgg cgaaggcctt tccgaaattg
     4021 ccgttcacct ggctcgattt cgaacggggt ggtggcggtg tgttcctgct gactcgagaa
     4081 cagttagaga aggcgtgagg ctgatgtcgg gtaactccat cggacgattg ttcaccgtga
     4141 cgacctttgg cgagagccac gggcccgcgc ttggctgcat cgtcgatggt tgtccgccgg
     4201 gactcgaact cagcgaggcc gacctgcaag gcgacgtcga tcgccgccgc acgggcacgt
     4261 cgcagttcgt gagccagcgt cgcgaaggcg acgcggtgaa gattctctcc ggtgtgttcg
     4321 aaggcaagac cacgggcacg gccattggca tcctcatcga gaacaccgac gcgcgctcac
     4381 gcgactacga aaagatcaag gatcgttttc gtccgggtca cgcggactac acctaccagc
     4441 agaaatacgg ctttcgcgat taccgtggtg gtggccgctc gtcggcgcga gaaaccgtca
     4501 tgcgtgtcgc ggccggcgcc atcgcgcgca aatacctgca cgctcggctc ggcgtcagca
     4561 tcagcggcta cctgagccag atcggctcgc tgaccatcga tccggtcaac cccgacgcgg
     4621 cctacgaaaa tcctttcttc tgtccggatc cgtcgcgcgt gcaggaactc gaagacctca
     4681 tgtggaagat ccgcggcgat ggcgattctc tgggcgccaa ggtcaccgtg gtcgcgcgcg
     4741 gcgtgccgcc ggg
//
LOCUS       DATDEU010000728         3739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1017249, whole genome shotgun sequence.
ACCESSION   DATDEU010000728 DATDEU010000000
VERSION     DATDEU010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017249"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1313)
                     /gene="waaA"
                     /locus_tag="VIV63_17340"
     CDS             complement(54..1313)
                     /gene="waaA"
                     /locus_tag="VIV63_17340"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEY6126422.1"
                     /translation="MRFLYICIAYLISPLVVGALALRGFRDRVHWRGFSQRFGFGETV
                     PSRSIWVHAVSVGEVQAGVPLVEALLKRFPTIPLVLTTVTPTGRSRAEALFGSRVDVR
                     YVPIDLPGSVRRFFQRAQPQLAIILETEIWPNLYHRCGRLGVPLVLASARISPRSVTS
                     YRRLVGLFRETLSHGIFIAAQSAEDAERFVSIGANPARTHVVGNIKFDFGYPPDMEAR
                     GHELRNLLGVHRPVWVAGSTHDQEEVQLLDAHAQIRQRFSHALMVLVPRHPPRFGEVA
                     SLLRSRGVPFVTRTSKESVTPDTQVFLLDTLGELPAFYAAGDVAFVGGSLVPIGGHNL
                     LEPAALSLPIVAGPHNFNSADIARMLVEHGAVRIVNDAMQLAEVVGSLLADPTTRTVM
                     GASGRKAVDGNRGAVARLMQFLEPLLP"
     gene            complement(1324..2085)
                     /locus_tag="VIV63_17345"
     CDS             complement(1324..2085)
                     /locus_tag="VIV63_17345"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HEY6126423.1"
                     /translation="MKRHWPVGAEVSEAAFEGGRMLYDTQRTSNFSADFFEPDYWRKH
                     DAIEGTARGRGTTWFIRAGDSSFVLRHYRRGGLMAKISRDKYWWHGENETRAFSEWYL
                     TYHLHRAGLPVPTPIAARYQRDGMFYRADIITQRIEDSEPLAARLLKGPLPLTQWIAV
                     GRCIRRFHDAGVCHADLNAHNILLTPGQVYLIDFDRGTLRKRGWWADTTLVRLYRSLE
                     KVTLLAAPDSFTDEDWHSMLAGYRESAGLPQASFV"
     gene            2098..3156
                     /locus_tag="VIV63_17350"
     CDS             2098..3156
                     /locus_tag="VIV63_17350"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY6126424.1"
                     /translation="MDSPEKVCLLRLSAIGDTCHVVPLLRTLQSAWPRTRFTWVIGKL
                     EAKLMSLIPDVELITVDKAAGLSAYRRLREDMKRRGAFDLLLHLQLSIRASATAACIP
                     APIKLGFDKPRAREMQWLFTNRRIAERSREHVLDSFLGFADALGVTRQQPRWDIPLPP
                     AALAYAEQLIPPSTSKDAQPTLVISACSSHRARNWLPERYAAVADHAVRNHGMRVILC
                     GGPAGIEREMAAAIAKHSTVPLIDQVGKDTLPQLLALLSRATVLLTPDSGPAHMSTMV
                     ETPVIGLYAATNPMRSGPYESRRWCVNAYDEAARKFLGKPAAELPWTQKIEQPGVMEL
                     ISVEAVAAKLDELLALLK"
     gene            3153..3551
                     /locus_tag="VIV63_17355"
     CDS             3153..3551
                     /locus_tag="VIV63_17355"
                     /inference="COORDINATES: protein motif:HMM:NF039364.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HEY6126425.1"
                     /translation="MKDILVPLSPGEMLDKITILRIKAARMSDPVKVANVTHELSLLE
                     KTWKDSGAAAVDLGPEEANLTRVNEALWVIEDDIRDEERAGRFGEKFIELARAVYITN
                     DERAAIKKRINTLLGSSIVEEKSYKPYKAE"
     gene            3554..>3739
                     /locus_tag="VIV63_17360"
     CDS             3554..>3739
                     /locus_tag="VIV63_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126426.1"
                     /translation="MKRRDVLTLAGASAALAALSPGRLFAADAVANLKSMTGDITTIP
                     RSEYLARIDKARALMGKL"
BASE COUNT          687 a         1258 c         1216 g          578 t
ORIGIN      
        1 tggaaacccc gccgccggcc gccgcgccgg tggtgccgcc gcccgcgcaa tagttagggc
       61 aacagcggtt ccaggaactg catgaggcgc gccaccgcgc ctcgatttcc gtccaccgcc
      121 ttcctgccac tcgcacccat cacggtgcga gtggtggggt cggccagcag acttcccacc
      181 acctccgcga gctgcatggc atcgttcacg atgcgcacgg cgccgtgctc gaccagcatt
      241 cgcgcgatgt cggcggaatt gaaattgtgc gggcccgcca cgatcggcag gctcagcgcc
      301 gcgggttcga gcaggttgtg gccgccgatg ggcaccaggc tgccgccgac gaaggccacg
      361 tcgcccgcgg cgtaaaacgc cggcagttcc ccgagtgtgt ccagcaggaa aacctgcgtg
      421 tcgggcgtga cgctttcctt gctggtccgc gtgacgaagg gcacgccgcg actgcgcagc
      481 aggctcgcga cttcgccgaa tcgcggcgga tggcgcggca ccagcaccat gagcgcgtgg
      541 gaaaaccgct ggcggatctg cgcatgcgcg tcgaggagct gcacttcttc ctgatcgtga
      601 gtacttcccg cgacccacac cggccggtgc acgcctaaca ggttgcgcag ctcgtggccg
      661 cgcgcttcca tgtcgggtgg atagccgaaa tcgaacttga tgttgcccac cacgtgcgtg
      721 cgcgccggat tggcgccgat ggatacgaaa cgctccgcgt cttcggcgct ctgcgccgcg
      781 atgaagatgc cgtgtgacag ggtctcgcgg aacaggccga ccagccggcg atagctggtc
      841 acggagcgcg gcgagatccg cgcgctcgcc agtaccagcg gcacgccgag ccggccgcaa
      901 cgatggtaga gattgggcca gatctccgtc tcgagaatga tcgccagctg cggctgggcg
      961 cgctggaaga agcgccgcac ggaacccggc agatcgatgg gcacgtagcg cacatcgacg
     1021 cgtgaaccga acaacgcctc ggcgcgtgag cggccggtgg gtgtcacggt cgtcagcacc
     1081 agcggtatcg tgggaaagcg cttgagcaag gcctcgacga gcggcacgcc ggcctgcact
     1141 tcgccgaccg acaccgcgtg cacccagatg ctgcgcgatg gcacggtttc gccaaacccg
     1201 aaccgttgcg agaaaccccg ccaatgcacg cgatcgcgaa aaccgcgcag cgccagcgcg
     1261 ccgacgacca gcggcgagat caggtacgca atgcagatgt agaggaagcg cacgcggaca
     1321 gcgtcagacg aaggaagcct gcggcaagcc cgcggactcg cgatatcccg ccagcatgga
     1381 atgccagtcc tcgtcggtaa aactgtcagg cgccgccagc agcgtcactt tctcgagcga
     1441 gcgatagagc cgcaccagcg tcgtgtccgc ccaccagccg cgcttgcgca gcgtgccgcg
     1501 atcgaagtcg atgagataga cctgccccgg cgtcagcagg atgttgtgcg cgttcagatc
     1561 cgcgtggcac acgccggcat cgtggaagcg ccggatgcag cggccgacgg cgatccactg
     1621 cgtgagcggc agcggaccct tgagcaggcg cgccgccagc ggctcgctgt cctcgatgcg
     1681 ctgcgtgatg atgtccgcgc gatagaacat cccgtcgcgc tggtagcgag ccgcgatggg
     1741 cgtgggcacg ggcagtcccg cgcgatgcag gtggtaggtg agataccact cggagaacgc
     1801 gcgggtttcg ttctcgccat gccaccagta tttgtccctg gaaatttttg ccatcaatcc
     1861 gccgcggcgg taatggcgca gcacgaagct gctgtcgccg gcgcggatga accacgtggt
     1921 gccgcggccg cgcgccgtgc cctcgatggc gtcgtgcttg cgccagtaat ccggctcgaa
     1981 gaaatccgcc gagaaattgc tcgtgcgttg ggtgtcgtat agcatccggc cgccctcgaa
     2041 ggcggcttcg ctgacttccg ctcccaccgg ccagtggcgt ttcattcact cacttccatg
     2101 gattcaccgg aaaaagtctg ccttctgcgg ttgtcggcca tcggcgatac gtgccacgtc
     2161 gtgccgctgc tgcgcacgct gcaaagcgcc tggccgcgga cgcgcttcac ctgggtgatt
     2221 ggcaaactcg aagcgaagtt gatgagcctc attccggatg tggagcttat caccgtggac
     2281 aaggctgccg gcctctccgc gtacaggcgc ctgcgggaag acatgaagcg gcgcggcgca
     2341 ttcgatctgc tgctgcacct gcaactgtcc atccgcgcca gcgcgaccgc cgcgtgtatc
     2401 cccgcgccta tcaagctcgg cttcgacaaa ccgcgcgcgc gcgaaatgca gtggctgttc
     2461 accaaccggc gcatcgccga aaggtcacgc gaacacgtgt tggacagctt cctgggattc
     2521 gccgatgcgc tcggcgtgac gcgccagcag ccgcgctggg acatcccgct gccgcctgcg
     2581 gcgctcgcgt acgccgaaca actgattccc ccttcgacct cgaaagacgc gcagccgacg
     2641 ctcgtgatca gcgcctgttc gagtcaccgc gcgcgcaact ggctgcccga gcggtacgcc
     2701 gccgtggccg accatgccgt gcgaaatcac ggcatgcgcg tgatcctgtg cggcggcccg
     2761 gccggcatcg aacgcgagat ggccgcggcc atcgccaaac acagcaccgt gccgctcatc
     2821 gaccaggtgg gcaaggacac gctgccgcaa ctgttggcgc tgctgtcgcg cgcgaccgtg
     2881 ctgctgacac ccgactccgg cccggcgcac atgtccacca tggtggagac acccgtcatc
     2941 gggctgtacg cggccacgaa ccccatgcgc agcggtccgt acgagtcgcg ccgctggtgc
     3001 gtgaacgctt acgacgaagc cgcgcgcaag ttcctgggca agccggccgc cgaactgccg
     3061 tggacgcaga agatcgaaca gcccggcgtc atggagctca tcagcgtcga ggccgtcgcc
     3121 gccaagctgg atgaactgct agccttgctc aaatgaaaga catcctggtc ccgctgagcc
     3181 cgggcgaaat gctcgacaag atcaccatcc tgcgcatcaa ggccgcgcgc atgagcgatc
     3241 cggtcaaggt cgccaacgtc acacacgagc tctcgctgct ggagaagacc tggaaggatt
     3301 ccggcgccgc cgccgtggat ctcggccccg aagaagccaa tctcacccgg gtgaacgaag
     3361 cgctctgggt catcgaggac gacatccgcg atgaagagcg cgccggccgc ttcggcgaga
     3421 agttcatcga gctcgcgcgc gccgtgtaca tcaccaacga cgaacgcgcc gccatcaaga
     3481 agcgcatcaa caccttgtta ggttcgagca tcgtcgaaga gaagtcgtac aagccgtaca
     3541 aggcggaata agcatgaaga ggcgcgatgt gttgacgttg gccggcgctt ccgccgcgct
     3601 ggcagcactg tcgccaggac ggctgttcgc cgcagacgcg gtggcgaacc tgaagtcgat
     3661 gacgggcgac atcacgacga tcccgcgttc cgagtacctg gcgcgcatcg acaaggcgcg
     3721 cgcgctcatg ggcaagctc
//
LOCUS       DATDEU010000729         6039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1164693, whole genome shotgun sequence.
ACCESSION   DATDEU010000729 DATDEU010000000
VERSION     DATDEU010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164693"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..389)
                     /locus_tag="VIV63_17365"
     CDS             complement(123..389)
                     /locus_tag="VIV63_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6126427.1"
                     /translation="MKFQFEKFYQAVNAKREERAASWRQVAREIGIGKSTLARMAQNK
                     RPDADGLAALSFWAELNPADFVSVEQIPVSSTVDCSVAANAMTA"
     gene            complement(582..1388)
                     /locus_tag="VIV63_17370"
     CDS             complement(582..1388)
                     /locus_tag="VIV63_17370"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY6126428.1"
                     /translation="MSWLEAIILGLVEGITEFLPVSSTGHLILTRDLLGLEGEAVDRQ
                     LLIIQGAAILAVCWEFRQKLLYTAFTLHSEAVSRRFVLNMLIAFMPLAVLGLLFGSQI
                     KAALFNPISVAIALIVGGVIILWAERRQHEERVRSVDDLTTLDAVKLGLFQALALIPG
                     TSRSGATIIGGLLCGLSRKTAAEFSFFVAIPVLLAATGYELWEARADLGSTDVDALIV
                     SCVVSFASALAATKLLIRFVSKHSFAAFAWYRIVFGAIILITAYTGIVSW"
     gene            complement(1434..2471)
                     /locus_tag="VIV63_17375"
     CDS             complement(1434..2471)
                     /locus_tag="VIV63_17375"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6126429.1"
                     /translation="MDARTKPASVAILALPEVGVSTVYGMFDMFMSAGRDWGVIVNGS
                     PGDSLIAPRVIATTREPFAAGNGVMIRADATLEEAGVPDVVCVPELLVAPGEPIDGRF
                     RAEVEYLKRCHDAGATLATACSGALLLAEAGLLDGHDATTHWAYCDILQRRYPKVRVH
                     AQRALVVAGAEQRLVMAGGGTSWLDLALYLIARLAGVEAAMQVARINLIDWHAVGQQP
                     FARLASSRQTDDAVIARCQGWIAEHYTAAGPVAQMTRLSGLAERSFTRRFREATGMAP
                     LEYVHTLRLEEAKQMLETGEQPVEGIANEVGYEDAAFFSRLFRRKVGLTPVQYRRRFG
                     SLRRSLTSGMP"
     gene            2562..3320
                     /locus_tag="VIV63_17380"
     CDS             2562..3320
                     /locus_tag="VIV63_17380"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY6126430.1"
                     /translation="MPTPLELLLDPLTLSVLAIFAVLAAWEALFPARVLPRVRAWRTK
                     GLVAFAAYMAISSYLPFLWAEWLAPFQWFDLTSLGTLAGAAVGVLFYEAGVWAWHRSL
                     HRSKTLWRYSHQWHHSAERLDTWGAFWFSPLDMVGWTALFSLALTLIGLAPQAVFATV
                     CVTTFLSVFQHANVRTPRWMGVLVQRPESHSWHHARGVHTGNYSDLPLFDMLLGTWHN
                     PRDFAPEQGFHPGASSQYSRLLLGRDVSATGEPS"
     gene            3317..4267
                     /locus_tag="VIV63_17385"
     CDS             3317..4267
                     /locus_tag="VIV63_17385"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY6126431.1"
                     /translation="MSQYRYRLPQLRNTLFLTDGGLETTLVFHDRMELPHFAAFDLLR
                     TAAGVERLRSYFETYTRIALDHRLGLVLEAPTWRANADWGAKLGYDAAALADVNRMSI
                     GLLLEVRAALETPATPIVISGNLGPRGDGYRADARMSVEEAMRYHSAQVETFAATDAD
                     MIGAFTMNYVEEAVGIALAARAHTMPLALSFTVETDGNLPSGQPLAEAIERVDAETDE
                     YPVYYMINCAHPSHFEGILDELGAKVRRVRGLRANASRRSHAELDASTDLDAGDPVTL
                     GSEYRALQRKLPALTVVGGCCGTDDRHVAAIARAMSHAGV"
     gene            complement(4264..5496)
                     /locus_tag="VIV63_17390"
     CDS             complement(4264..5496)
                     /locus_tag="VIV63_17390"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEY6126432.1"
                     /translation="MQVYLVGGAVRDRLLGREVSERDWVVVGATPAELERSGFIPVGR
                     DFPVFLHPKTKEEHALARLERKIAPGYRGFATEFAPDVTLEDDLKRRDLTINAMAQDD
                     AGNIIDPHGGQADLAARQLRHVSDAFIEDPVRILRVARFAARYAPLGFRVADETMALM
                     QKMVADGEADALVAERVWAETEKALGEASPDVFISVLRHCGALKVVYPEIDALFGVPQ
                     PAKWHPEIDTGVHQLMALRMAVKLGGGVAVRFAVLMHDLGKAATPVDKLPSHPGHEDA
                     GVALVEKLADRLRVPNHLRELAVMTSRYHTHVHRAAELRPGTVLSTLEGCDALRRPER
                     FADFLLACEADARGREGLEDRGYPQREYFAAARAAAAAVVLTAEERAGRSGEQIGQEL
                     RQRRIAAVAALKAATAPS"
     gene            5495..>6039
                     /locus_tag="VIV63_17395"
     CDS             5495..>6039
                     /locus_tag="VIV63_17395"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY6126433.1"
                     /translation="MARMVTIRTALVLFTCLSLTGCGTTYVAQAAKGQLQILTARKPI
                     AKVLDDPEADPGLKKRLEEVREAREFASRELGLPNNKSYTAYADLKREFVVWSVVATP
                     EFSVDPREWCFPIVGCVAYRGYFRKESAEAFAETLRSEGLDTMVGGVPAYSTLGKFND
                     PILNTMMAYGDDELASIMFHEL"
BASE COUNT         1015 a         1992 c         1993 g         1039 t
ORIGIN      
        1 gagctcgaga cgggtccgca cggcgagttc atcgtggtgt tgcacggcgg cgcgcggctg
       61 cagtcgagcc gcagctacca cgagaagatc aagcgctggg ccgcgaaccc tttctgacgt
      121 ggctaggccg tcatcgcgtt cgcggcgacg gagcagtcga cggtcgagct gaccgggatc
      181 tgttccaccg acacgaaatc cgccgggttg agttccgccc agaacgacag cgccgcgagg
      241 ccgtcggcgt cgggacgctt gttctgcgcc atgcgcgcca gtgtggattt gccaatgccg
      301 atctcgcgcg cgacctgccg ccacgaggcc gcgcgctctt cgcgcttcgc attgaccgcc
      361 tgatagaact tttcgaattg gaacttcatg cgcggaagtt tcacctccgc gcggcgaccc
      421 ggaaaagtga attttgacga agtggatgtt ttcggtgacg gatatgggga cattcctgat
      481 ttgcagcaaa acgggacgcg gctcgggaac cggttcctca tgagatgaca cggcgcgtct
      541 gagccgcgtc cccttttgct agcaaatcag gaatgtcccc attaccagct cacgatgccg
      601 gtgtacgcgg tgatcaggat gatggcgccg aacacgatgc ggtaccacgc gaacgccgcg
      661 aacgaatgtt tgctgacgaa acggatcagc agtttggtgg cggcgagcgc gctcgcgaac
      721 gagacgacgc agctcacgat gagcgcgtcg acatcggtgg aaccgagatc cgcgcgcgct
      781 tcccacagtt cgtagcccgt cgcggccagc agtaccggga tcgcgacgaa gaacgagaac
      841 tccgccgcgg tcttgcgcga cagcccgcac agcagtccgc cgatgatcgt cgcgcccgag
      901 cggctcgtgc cgggtatcag cgccagcgcc tgaaacaggc cgagcttcac cgcgtcgagc
      961 gtggtgaggt cgtccacgct gcgcacgcgc tcctcgtgct ggcggcgttc ggcccacagg
     1021 atgatgacgc cgccgacgat cagcgcgatg gccacggaga tgggattgaa cagcgccgcc
     1081 ttgatctggc tgccaaacag caggccgagc acggcgagcg gcatgaacgc gatgagcatg
     1141 ttgagcacga agcgccggga cacggcttcg ctgtgcagcg tgaacgccgt gtacagcagt
     1201 ttctgtcgaa attcccagca gaccgcgagg atcgccgcgc cctggatgat gagcagctgg
     1261 cggtcgaccg cttcgccctc gagaccgagc aggtcacgcg tgaggatgag gtggccggtg
     1321 ctcgagaccg gcaggaactc ggtgatgcct tcgacgaggc cgaggatgat cgcttcgagc
     1381 caggacatgc gcgcgcttca gtgttcgatt cgggcgcgca gtttaccgga gcttcacggc
     1441 atcccgctcg tcagactgcg ccgcaaactg ccgaagcgcc ggcgatattg caccggtgtg
     1501 agcccgacct tgcggcggaa cagccgcgaa aaaaacgcgg catcttcgta gcccacttca
     1561 ttggcgatac cttcgaccgg ctgctcgccc gtttcgagca tctgcttggc ttcttcgagc
     1621 cgcagcgtgt gcacgtattc gagcggcgcc atgccggtgg cttcgcggaa acgccgcgtg
     1681 aacgagcgtt cggcgaggcc cgacagccgc gtcatctgcg ccaccggtcc cgcggcggtg
     1741 taatgctcgg cgatccagcc ctggcagcgc gcgatgaccg cgtcgtcggt ctggcgagaa
     1801 ctcgcgaggc gcgcgaacgg ctgttggccc acggcatgcc agtcgatgag attgatgcgc
     1861 gccacctgca ttgcggcttc tacgccggcg agacgcgcga tgagatagag cgcgaggtcg
     1921 agccacgacg ttccaccgcc cgccatcacc agccgctgct ccgccccggc gaccaccagc
     1981 gcgcgttgtg cgtgcacgcg cactttggga tagcgccgct gcaggatgtc gcaataggcc
     2041 caatgcgtgg tcgcgtcgtg gccgtctaac aacccggcct cggccagcag caacgcgccc
     2101 gagcacgcgg tggccagcgt ggcgccggca tcgtggcaac gtttgaggta ttccacttcc
     2161 gcccgaaacc gcccgtcgat cggttcgccg ggagccacca gcagttccgg aacgcacacg
     2221 acgtcgggca caccggcttc ttcgagcgtg gcatcggcgc ggatcatcac gccgttgccc
     2281 gcggcgaacg gctctcgcgt ggtggcgatg acgcgcggcg caatcagcga atcgccggga
     2341 cttccattga cgatcacacc ccagtcccgg cccgccgaca tgaacatgtc gaacatgccg
     2401 tagaccgtgg atacaccgac ttccgggagg gcgaggatcg cgacgctggc gggtttggtc
     2461 cgggcatcca tggcggaatc gtcaggtttg tggccggtga ggcttgagtg aattcgcggc
     2521 gggcacttta ccttgcgcgc ccccgaataa ggagcttcga catgccaacc ccactcgagc
     2581 tgttgctcga ccccctgacc ctctccgtcc tcgccatctt tgccgtcctc gcggcctggg
     2641 aggcgctgtt cccggcacgc gtcctgccgc gcgtacgcgc ctggcgcacc aagggcctcg
     2701 tggcctttgc cgcgtacatg gccatctctt cctatctgcc ttttctctgg gcggaatggc
     2761 tggcgccgtt tcagtggttc gatctgacca gccttggcac gctcgccggc gccgcggtcg
     2821 gcgtgctgtt ctacgaagcg ggcgtctggg cctggcaccg cagcctgcac cgctcgaaga
     2881 cgttgtggcg ctattcgcac cagtggcatc acagcgccga gcgcctcgat acctggggtg
     2941 cgttctggtt cagtccgctc gacatggtgg gctggacggc gctcttcagc ctcgcgctca
     3001 cgctgatcgg ccttgcgccg caggccgtct tcgccacggt ttgcgtgacc acgttcctgt
     3061 cggtgttcca gcatgccaat gtgcgcacgc ctcgctggat gggcgtactg gtgcagcgac
     3121 cggagagtca ttcgtggcat cacgcacgtg gcgtgcacac cggcaactac tccgaccttc
     3181 cgctcttcga catgttgtta ggcacctggc acaacccgcg cgacttcgcg ccggagcagg
     3241 gctttcaccc gggcgcttcg tcgcagtaca gtcgcctgct tctcggccgc gacgtctcgg
     3301 ccaccggaga accgtcatga gccagtaccg ataccgattg ccgcagctgc gcaacacgct
     3361 gttcctgacc gatggcggtc tcgagaccac gctagtgttc cacgaccgga tggagctgcc
     3421 gcatttcgcg gcgttcgacc tgctgcgaac cgcggcgggt gtggagcgtc tgcgcagtta
     3481 tttcgaaacc tacacccgca tcgcgctcga tcaccggttg gggctcgtgc tcgaggcgcc
     3541 gacgtggcgc gcgaatgccg attggggcgc gaagctgggt tacgacgcgg cggcgctggc
     3601 cgacgtcaat cgcatgagca tcggcctgct gctcgaagtg cgggcggcac tcgaaacgcc
     3661 ggcgacgccc atcgtgatca gcggcaacct gggcccgcgc ggcgacggtt atcgcgccga
     3721 tgcgcgcatg agcgtcgaag aggcgatgcg ttatcacagc gcgcaggtgg aaacgttcgc
     3781 ggccaccgac gccgacatga tcggcgcgtt caccatgaac tacgtggagg aagccgtggg
     3841 catcgcactg gcggcgcgag cgcacaccat gccgctggcg ctgtcattca ccgtggagac
     3901 cgacggcaat ctcccgtccg gccagccgct ggcggaagcc atcgagcgcg tcgacgcgga
     3961 aacggatgag tacccggtgt actacatgat caactgcgcg catccctcgc acttcgaggg
     4021 catcctggac gagctgggcg cgaaggtgcg ccgagtacgc ggtctgcgcg cgaacgcctc
     4081 gcggcgcagt catgccgaac tcgatgcgtc gaccgacctc gatgcgggcg atccggtcac
     4141 gctgggcagt gaataccgcg cactccaacg caagttgccg gcgctgaccg tggtgggtgg
     4201 ctgttgcggt acggatgatc ggcacgtggc ggccattgcc agggcaatga gccatgccgg
     4261 cgtttaggaa ggggcggtcg ctgccttcaa cgcggcgacg gcggcgatgc gccgctggcg
     4321 aagttcctgg ccgatctgct cgcctgaacg gccagcgcgc tcttcggcgg tgagcacgac
     4381 ggcggccgcg gcggcgcgtg ccgccgcgaa atactcgcgt tgcgggtagc cgcggtcttc
     4441 aagtccctcg cggccgcgcg catcggcttc gcaggcgagc aggaagtccg cgaagcgctc
     4501 ggggcgacgc agggcgtcgc aaccttcgag cgtgctcagc acggtcccgg gacgcagctc
     4561 cgcggctcga tgcacgtgcg tgtgataacg cgacgtcatc accgcgagtt cgcgcaggtg
     4621 gttagggacg cgcaggcgat cggccagttt ttccacgagc gccacgccgg catcttcatg
     4681 gcctggatgc gagggcagtt tatcgacggg tgtggccgcc ttgccgaggt cgtgcatgag
     4741 cacggcgaaa cgcaccgcca cgccgccgcc taacttcacg gccatgcgca gcgccatgag
     4801 ctggtggacg ccggtgtcga tctcggggtg ccacttcgcg ggctgcggca cgccgaacaa
     4861 cgcgtcgatc tcgggataca ccaccttgag cgcgccgcag tggcgcagca cgctgatgaa
     4921 cacgtccggg ctcgcctcgc ccagcgcctt ttcggtttcg gcccacacgc gctcggcgac
     4981 cagtgcgtcg gcctcgccgt cggccaccat cttctgcatg agcgccatgg tttcatcggc
     5041 cacgcgaaaa cccaggggcg cataacgcgc ggcgaaacgc gccacgcgca ggatgcgcac
     5101 cgggtcttcg atgaatgcgt cggaaacgtg gcgcaactgg cgggcggcga gatcggcctg
     5161 gccgccatgc gggtcgatga tgttgccggc gtcgtcctgc gccatcgcgt tgatggtgag
     5221 atcgcgccgc ttgaggtcgt cctcgagcgt cacatcgggc gcgaactcgg tggcgaaacc
     5281 gcggtaaccc ggcgcgatct tgcgttccag gcgcgcgagc gcgtgttctt ccttggtttt
     5341 cgggtgcagg aacaccggga agtcccggcc cacgggaatg aaccccgagc gctcgagttc
     5401 cgccggcgtg gcgcccacga cgacccagtc gcgctcggac acttcgcgtc cgagcaaccg
     5461 gtcccgaacc gcgccgccca ccaagtacac ttgcatggct cgcatggtaa ccattcgtac
     5521 tgccctcgtg ctcttcacct gcctaagcct tacgggctgc ggcactactt acgtggcaca
     5581 ggcagccaag ggacagctgc agatcctcac cgcgcgcaag cccatcgcga aagtgctgga
     5641 cgatccggaa gcggatcccg ggctcaagaa gcgcctcgaa gaggtacgcg aggcccgcga
     5701 attcgcctcg cgggagcttg ggctgcctaa caacaagagc tataccgcgt acgccgatct
     5761 gaagcgcgaa ttcgtggtgt ggagcgtggt ggcgacgccg gagttctcgg tcgacccgcg
     5821 cgaatggtgt tttccgatcg tcggctgtgt ggcctaccgc ggctatttcc gcaaggaaag
     5881 cgccgaggcg tttgccgaaa ccctgcgatc cgagggcctc gacaccatgg tcggcggcgt
     5941 acccgcgtat tccacgctcg gcaaattcaa cgatcccatc ctcaacacca tgatggccta
     6001 cggtgacgac gagctcgcgt ccatcatgtt ccacgagct
//
LOCUS       DATDEU010000730         5394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_726173, whole genome shotgun sequence.
ACCESSION   DATDEU010000730 DATDEU010000000
VERSION     DATDEU010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726173"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VIV63_17400"
     CDS             complement(<1..683)
                     /locus_tag="VIV63_17400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6126434.1"
                     /translation="MTETEGPSPNARRFAGIALAPGYLPAHAATYLYAAFITIGLLAF
                     VSYIQPYLLTVNLGLPAHLQGRTISALNVANELLALCLVAPFGALADKIGRRPVYAFG
                     FLWLAAGFLLYPMARTVMQLTGCALFFSIGVAAIGTMLGTVLADTPAEQSRGRLVGLT
                     GFFQGLGAAALVLLLGRLPKHLADSGVDALTAGRITLWLAAALCLVSSVVVFAGLKRG
                     TPSERAPAMP"
     gene            830..2887
                     /locus_tag="VIV63_17405"
     CDS             830..2887
                     /locus_tag="VIV63_17405"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY6126435.1"
                     /translation="MTRYVCAAAAALMVFLCSTSRADEGMWTFHGFPFAKANAALKTS
                     LDQPWLDRVRSATVRLSNCTATFVSNEGLILTNHHCVEACLAELSSKEKSLVEDGFLA
                     KTREDEKRCQTQVADVLIGMEEITAKVSAATAGKDETAANEIRKSTLTQLEGDCEKQA
                     TQKCQTVTLYQGGQYWLYKYKRYTDVRIVFAPENSIAAFGGDPDNFQFPRWCLDMGLL
                     RAYEDGKPVKPSSFLKLNFAGPSVGEAVFVSGHPGATDRLLTLAQLKQQRSLELPQWL
                     LRYSEIRGRLIQFSQTSPENERITEELLNSLENGIKVRRKMQDALNEDSLFVRKAAEQ
                     DALQKSVAKDAALKKSTGNPWADIDAALATERGIFLPYTYLENGAGFQGRLVGYARAL
                     VRGTVERDKPNNQRIREFTDGSLPRIQQNLSAQVPVYPELEILRLGNSLERMREWLGP
                     DHPVVRKLMSKESPAALAKRLVTETKLADPAARLELWKAGSAAVAASTDPMIVLVRSI
                     DAETRAVRKAYEDGVEAPMRSATERIARARFASLGTSVYPDATFTLRLNYGTVQGWNE
                     NGKPVEPFTRLSRVFERATGSDPFRVPESWERVKGKLDMNTPFNLSTNNDIVGGNSGS
                     ALINAKAEIVGLMFDGNIHSISGAYWFDTEKNRAIAVHPAIMREAMSKVYGADALLAE
                     LTK"
     gene            complement(2930..3673)
                     /locus_tag="VIV63_17410"
     CDS             complement(2930..3673)
                     /locus_tag="VIV63_17410"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY6126436.1"
                     /translation="MSTQPKGRGFPLGVVVAGLTLWGLTGCQTSPPAADTPVPEAAPA
                     APSAVPADAREYQVVAEESLLQILVYRGGAMARLGHNHVIASHHLSGAVFVANDVSKS
                     RFDISVPVNDLTVDEPAMREMAGADFPPGVPQSARDGTRKNLLSEALLDGAKYPAIRL
                     RATDIVAASAGFDVGVEITIKDQVRNVRVPVTFESRDGAVVARGEFPLKQSELGFKPF
                     SVAMGTLVVLDEMRIHFEVTAKMGTLPIS"
     gene            3733..4287
                     /locus_tag="VIV63_17415"
     CDS             3733..4287
                     /locus_tag="VIV63_17415"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY6126437.1"
                     /translation="MTFRNTTRSWGSLTKALHWIIVILIVAQFLIASRAHSLPRGPAL
                     IEAWGWHKSVGMTIFMLAVFRLVWRWMNPVPDLSSEVKPWEQLLAKLSHVLLYTLLFA
                     VPLSGWLMSSAKNFPVSWFGLFQWPDLVAPDSAFSKTMEATHETLVKVLFFVALLHMA
                     GALKHHFIDKNDVLKRMLPFGGVK"
     gene            4288..4857
                     /locus_tag="VIV63_17420"
     CDS             4288..4857
                     /locus_tag="VIV63_17420"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY6126438.1"
                     /translation="MRLTNYLAPLAVLWLAGTAYAADAPRWVQAPTGSSLVFVFDQAG
                     AASKGSFKQFTTELAYDEKSPAAGSLHVKVQIGTVDTQDQERNDMLVGSDLFDAPKFP
                     TAQFVSSSFVRSAAGGLEAVGKLTLRGVTRDLRLPLKIVPTKAGLELSGEATIKRLDY
                     GVGQGDWKTTEGVGDEVKVQYKVALVKAK"
     gene            4860..5393
                     /locus_tag="VIV63_17425"
     CDS             4860..5393
                     /locus_tag="VIV63_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126439.1"
                     /translation="MSMTKSMRQVWMGLSLSIFLLSFFPAWTNRISWWMPGVAFVLFA
                     IFLLEPIFLTWELRKRGEALQITDEGVLRRLARGDSEFVRWSELREVSIVMTQGVSMS
                     EEYFYVLAGAGSTGVLVGQALAAKYDLLSHLAKLPGFDHRNITAAMASPVNQKFTLWQ
                     AAPLQGEAVVLPNRALN"
BASE COUNT         1014 a         1746 c         1705 g          929 t
ORIGIN      
        1 ggcatggcgg gcgcccgctc gctgggtgtg ccgcgcttca ggccggcgaa taccaccacg
       61 gaggagacga ggcacaacgc cgccgcgagc cacagcgtga ttcgccccgc cgtgagtgca
      121 tcgacgcccg agtcggccag gtgcttcggg agtctcccaa gcaacagcac cagcgccgcg
      181 gcacccaggc cctggaagaa tcctgtgagg cccacgaggc ggccgcgcga ttgctccgcc
      241 ggcgtgtccg ccagcaccgt tcctaacatc gtgccgatgg cggcgacccc aatggaaaag
      301 aacagcgcgc aaccggtgag ctgcatgacg gtgcgcgcca tcgggtacag cagaaatccc
      361 gcggccagcc acaggaagcc gaaggcgtat accgggcggc gcccgatctt gtccgccagt
      421 gcgccgaacg gcgccaccag gcagagcgcc aacaactcat tggccacatt gagggcggag
      481 atggtccggc cctgaaggtg ggcgggaaga cccaggttca cggtcagcag gtaaggctgg
      541 atgtagctca cgaaagccaa cagcccgatc gtgatgaatg cggcgtatag ataggtggcc
      601 gcgtgcgctg gcaggtatcc cggcgcgagt gcgattccgg caaagcggcg cgcgttggga
      661 ctgggacctt ctgtttctgt cacggcgggg caaatcatgc catgcccggt cgcgacgaac
      721 cgagcgcggg acgcgtcatt ccgggcatgc gcacatggat gattgtggtg ataatcggcg
      781 catagaccgc cccttcgggt cggcaccaga aattcacgga gttccgttca tgactcgata
      841 cgtgtgcgcg gccgccgccg ccctgatggt tttcctgtgc tcgacttccc gcgccgacga
      901 aggcatgtgg acgtttcacg gcttcccgtt cgccaaggcc aacgcggcgc tgaagaccag
      961 tctggatcag ccgtggctcg atcgagtgcg ttcggccacc gtgcgcctgt ccaactgcac
     1021 cgccacgttc gtctccaatg aaggcctgat ccttaccaac catcattgcg tcgaagcctg
     1081 tctcgccgag ctgtcgtcga aggaaaaaag tctcgtggaa gacggtttcc tcgcgaagac
     1141 ccgcgaagac gagaagcgat gccagacgca ggtcgccgat gtgctcatcg gcatggaaga
     1201 gatcaccgcg aaggtgtcgg cggccacggc cggcaaggac gagaccgccg cgaacgagat
     1261 ccgcaagtcc acgctgacgc agctcgaagg cgactgcgag aaacaggcga cgcagaagtg
     1321 ccagacggtc acgctgtacc agggcggcca gtactggctg tacaagtaca agcgttacac
     1381 cgacgtgcgc atcgtgttcg cgcccgaaaa cagcatcgcg gctttcggcg gcgaccctga
     1441 caacttccag tttccgcgct ggtgcctcga catgggactg ctgcgcgcct acgaggacgg
     1501 caagccggtc aagccttcga gcttcctcaa gctcaatttc gcgggcccgt cggtcggcga
     1561 agcggtgttc gtgtcgggtc atcccggcgc caccgatcgt ctgctgacgc tggcgcagct
     1621 caagcagcag cgcagcctcg aacttccgca gtggcttctg cggtactcgg aaatccgcgg
     1681 ccggctgatc cagttctcgc agacgagccc ggagaacgag cgcatcaccg aggaacttct
     1741 caacagcctc gagaacggca tcaaggtacg ccgcaagatg caggacgcgc tcaacgaaga
     1801 ctcgctgttc gtgcgcaagg ccgcggaaca ggatgcgttg cagaagagcg ttgcgaaaga
     1861 tgcggcgctg aagaagagca cgggcaatcc gtgggccgac atcgacgcgg cattggccac
     1921 cgaacgcggc atcttcctgc cgtacaccta tctcgagaac ggcgcgggat tccagggccg
     1981 cctcgtcggt tatgcgcgcg cgctggtccg cggcacggtg gagcgcgaca agcctaacaa
     2041 ccagcgcatc cgcgaattca cggacggctc gttgccccgc atccagcaga atctgagcgc
     2101 gcaggtgccg gtgtatcccg aactggaaat cctcaggctc ggcaacagcc tcgagcgcat
     2161 gcgcgaatgg ctgggccccg atcatccggt ggtgcgcaag ctcatgtcga aggaatcgcc
     2221 cgcggcgctc gccaaacgtc tcgtgaccga gacgaagctg gcggatccgg ccgcgcgcct
     2281 cgagctctgg aaagccggtt cggcggccgt cgccgcgtcg accgatccca tgatcgtgct
     2341 ggttcgcagc atcgacgcgg agacacgcgc ggtccgcaag gcttacgaag acggcgtcga
     2401 agcaccgatg cgctcggcca cggaacgaat cgcgcgcgcc cgcttcgcct cgctgggcac
     2461 cagcgtgtac ccggatgcca cgttcacgct gcgcctcaac tacggcacgg tgcagggctg
     2521 gaacgagaac ggcaaaccgg tggagccatt cacgcggctg tcgcgcgtat tcgaacgcgc
     2581 gaccggctcg gatccgtttc gcgtcccgga aagctgggag cgcgtgaagg gcaagctcga
     2641 catgaacacg ccgttcaacc tgtcgaccaa caatgacatc gtcggcggca actcgggcag
     2701 cgcgctcatc aacgcgaagg ccgagatcgt cggcctgatg ttcgacggca acatccattc
     2761 gatttccggc gcgtactggt tcgacaccga gaagaaccgc gccatcgccg ttcacccggc
     2821 gatcatgcgc gaggccatgt cgaaggttta tggcgccgac gccttgctgg cggagctgac
     2881 caaataggga tagcgggggc cggagcttca agagctccgg ccccttttgc taggaaatgg
     2941 ggagtgtccc cattttcgcg gtgacttcga agtggatgcg catttcgtcg agcaccacca
     3001 gcgtacccat ggcgacgctg aagggtttga atcccaactc tgactgcttg agcggaaatt
     3061 cgccgcgcgc caccaccgcg ccgtccctgc tttcgaacgt cacgggcaca cgcacattgc
     3121 gcacctggtc cttgatggtg atctccacgc cgacgtcgaa gcccgcactc gccgcgacga
     3181 tgtcggtggc gcgaagacga atggccggat acttcgcgcc gtcgagcagc gcttccgaca
     3241 acaggttctt gcgcgttcca tcgcgcgcgc tttgcggcac gcccggcggg aagtcggcac
     3301 cggccatttc gcgcatcgcc ggttcgtcga cggtcaggtc gttcacgggc acgctgatgt
     3361 cgaagcgcga cttcgagacg tcgttcgcga cgaacaccgc gcccgacagg tggtgcgacg
     3421 caattacgtg gttgtgaccg agtcgcgcca tggcgcctcc gcgatacacg aggatctgca
     3481 gcagcgactc ctcggcgacc acctggtatt cacgcgcatc cgcgggaacc gccgaaggcg
     3541 ctgccggcgc ggcttcggga accggggtat ccgccgcggg gggacttgtc tgacacccgg
     3601 tcagccccca tagcgtgagg cccgcaacaa ccactcccag cgggaacccg cgacccttcg
     3661 gctgtgtact catgaatatg cttcatcctt ggctctttga cctggctcat cggtagcggc
     3721 tagcttaccg acatgacttt tcgtaacacc acacgctcct ggggttccct cacgaaggcg
     3781 ctgcactgga tcatcgtgat cctcatcgtc gcgcagttcc tcatcgcatc gcgcgcgcat
     3841 tcgctgccgc gcggcccggc actcatcgag gcctggggct ggcacaagtc cgtcggcatg
     3901 accatcttca tgctcgcggt tttcaggttg gtatggcgct ggatgaatcc ggtccccgac
     3961 ctcagcagcg aggtcaaacc ctgggagcaa ctgctcgcga agctgtcgca cgtattgctc
     4021 tacacgttgc tgttcgcggt gccgctcagc ggctggctga tgtcctccgc caagaacttt
     4081 ccggtgagct ggttcgggct cttccagtgg cccgacctgg tcgcccccga ctcggcgttc
     4141 tcgaaaacca tggaagcgac ccacgagacg ctggtcaagg tgctgttctt cgtcgcgctg
     4201 ctgcacatgg ccggcgccct caaacaccat ttcatcgaca agaacgatgt gctcaaacgc
     4261 atgttgcctt tcggcggagt caaataagtg cgactgacta actatctggc tccgctggcc
     4321 gtgttgtggc tcgccggcac cgcgtacgcc gccgatgccc ctcgctgggt gcaggcgccc
     4381 accggcagca gcctcgtttt cgtcttcgat caggccggcg ccgccagcaa gggctcgttc
     4441 aagcagttca ccacggagct cgcctacgac gaaaagtcgc ccgccgccgg aagcctccac
     4501 gtgaaggtgc agatcggcac ggtcgacacg caggaccagg agcgcaacga catgctcgtg
     4561 ggttcggacc tcttcgatgc accgaaattt ccgacggcgc aattcgtctc cagcagcttc
     4621 gtgcgcagcg ccgccggcgg cctggaggcc gttggcaaac tgactttgcg cggcgtcacg
     4681 cgtgacctgc gcctgccgct caagatcgtg ccgaccaagg ccgggctcga gctttccgga
     4741 gaagccacca tcaagcgtct cgactatggc gtcggccagg gcgactggaa aaccaccgaa
     4801 ggcgtcggcg acgaagtgaa ggttcaatac aaagtcgcgc tggtgaaggc gaaatagcca
     4861 tgtcgatgac gaaatccatg cgccaggtct ggatgggcct gtcgctcagc atcttcctgc
     4921 tgtcgttctt cccggcctgg accaaccgca tcagctggtg gatgccgggt gtcgcgttcg
     4981 tgctgttcgc catcttcctg ctcgaaccca tctttctcac ctgggagctg cgcaaacgcg
     5041 gagaagcgct gcagatcacc gatgaaggcg tgttgcgccg gctggcgcgt ggcgacagcg
     5101 agttcgtgcg ctggagcgaa ttgcgcgaag tgtcgatcgt gatgacgcag ggcgtctcga
     5161 tgagcgagga atatttctac gtgctggcgg gcgccggctc gaccggcgta ctcgtcggcc
     5221 aggcgctggc ggcgaagtac gacttgctct cgcacctcgc caaactaccc ggtttcgatc
     5281 atcgcaacat caccgcagcc atggcgtcgc cggtgaatca gaagttcacg ctgtggcaag
     5341 cggcgccgct ccagggtgaa gcggtggtgt tgccgaatcg cgcgctgaac tagc
//
LOCUS       DATDEU010000731         2813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_432121, whole genome shotgun sequence.
ACCESSION   DATDEU010000731 DATDEU010000000
VERSION     DATDEU010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432121"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VIV63_17430"
     CDS             complement(<1..673)
                     /locus_tag="VIV63_17430"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6126440.1"
                     /translation="MSTRPTLLIVSYHFAPSPLVGAKRFSFLTREFTRLGFDVHVIAN
                     GIKESPHGSQDGSLPISGTVHRVDAPFEVPLKGNGLLRRAANAVFRRLLAPVGMEYFW
                     ARAATRKALAVARTLPPGIVIATSPPHAALIAGARVARRLKWPLILDYRDPWSAYDWP
                     EWHRGWLTQWLAARIERRLVRQSSARVLNTPSMRDWFEESFPQAPAATNFVIPNGFDA
                     VAAAGA"
     gene            complement(670..2103)
                     /locus_tag="VIV63_17435"
     CDS             complement(670..2103)
                     /locus_tag="VIV63_17435"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY6126441.1"
                     /translation="MSSFVSRGAVLASARLLNQALALLSPLLLVRLLEISEYGRYRQF
                     MATAMFITSLAGFALSANLNYLIARSPDRQAVDISNTCLLMLGVSVASALIIVVFQQW
                     IVPPEISQAWVLLALYVFLFLNLEVLVSYWLAHGQSVAVMGYTLAVTVWRIATLLAAT
                     YWLHDVQLIFVTIVCAEAFKNLWIYVGLRRRGLLVFRWDAAAMREQIRLVAPLGAGSV
                     LYKANDFGKVVVANQMGPVPLAIYTTAAYQVPLVNIVQGALADAIFPDMVKRSKRDPA
                     QGLLLWKRAQVLIFAVICPAWLLLTYWAEPFVRLLFTEQYVAATPYFQFFLLLMVRQC
                     FQFSTPLRSVEDNASFAHANLIALVINATLIVTLMPKFGLWGPTLGLVVGQTWTSFYL
                     GSRVLKRYKLPVSELCQWGKLGLALMASVVALAAMYATLFYLPDTAASIVASLGVFAL
                     VYAIAARVILREEYGYVMRAILRRKTA"
     gene            2249..2677
                     /locus_tag="VIV63_17440"
     CDS             2249..2677
                     /locus_tag="VIV63_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126442.1"
                     /translation="MKFKTAEARLRVLHPVTAEATQLPSRDDLPAIPAMSRMIELMEM
                     AAARLMRPRLQNGESSVAVAMNVTYAAPQMRLPGAASTLRAVASYKEISGRLHRFTIN
                     AFDESGLVGTAEHTRAVLAERRLSDVARQQAGKTGERLIA"
BASE COUNT          563 a          897 c          923 g          430 t
ORIGIN      
        1 gcgctccggc cgcggccacg gcgtcgaagc cgtttggaat cacgaaattc gtggcagcgg
       61 gcgcctgcgg aaaagattcc tcgaaccagt cgcgcatgct cggcgtgttg agcacgcgcg
      121 ctgaactctg gcgcaccagg cggcgctcga tgcgcgccgc cagccattgc gtgagccagc
      181 cgcggtgcca ctcgggccag tcgtacgcgc tccacggatc ccggtagtcg agtatcagcg
      241 gccacttcag gcgacgcgcg acgcgtgcgc ccgcgatgag cgccgcgtgt ggcggtgacg
      301 tggcgatgac gatgcccggc ggcagtgtcc gcgccaccgc gagtgctttg cgcgtcgcgg
      361 cgcgagccca gaaatattcc atgcccacgg gcgcgagcag ccggcgaaat accgcattgg
      421 cggcgcgccg aagaaggcca tttcccttga gcggcacttc gaacggcgcg tcgacgcgat
      481 gcacggttcc actgatgggc agcgaaccgt cctgacttcc atgcggcgat tccttgatgc
      541 cgttggcgat gacgtggacg tcgaacccca ggcgagtgaa ttcgcgcgtg agaaagctga
      601 atcgcttggc gccgaccagc ggactcggtg cgaagtgata cgacacgatg agcagggtgg
      661 ggcgcgtgct catgcggtct tccggcgcag gatcgcgcgc atcacgtagc catattcctc
      721 gcgcaggatg acgcgcgcgg cgatggcgta caccagcgcg aacacgccga gcgacgcgac
      781 gatgctggcc gcggtatccg gcaggtagaa cagcgtggcg tacatcgcgg cgagcgcgac
      841 caccgacgcc atcaacgcaa gtcccagctt gccccactgg cagagttccg acaccggtag
      901 tttgtagcgc ttgagcacgc gcgacccgag atagaacgac gtccaggtct gtcccacgac
      961 cagcccgagc gtcggacccc acaggccgaa tttcggcatc agcgtgacga tgagcgtggc
     1021 attgatcacg agcgcgatga gattggcgtg cgcgaacgac gcgttgtcct cgacgctgcg
     1081 caagggggtc gagaactgga agcactgccg caccatgagc agcaggaaga actggaagta
     1141 gggagtggcc gccacgtatt gctcggtgaa cagcagtcgg acgaaaggtt cggcccaata
     1201 ggtgagcaac agccaggccg ggcagatgac ggcgaagatc agcacctgcg cgcgtttcca
     1261 gagcaacagg ccctgcgcag gatcgcgctt cgaacgtttg accatgtcgg ggaagatcgc
     1321 gtcggccagc gcgccctgca cgatgttcac cagcggcacc tggtaggccg cggtggtgta
     1381 gatagccagg ggcacgggcc ccatctggtt ggccacgacc accttgccga agtcgttggc
     1441 cttgtagagc accgacccgg cgccgagcgg cgcgaccagg cgtatctgct cgcgcatcgc
     1501 ggccgcgtcc cagcggaaca cgagcaggcc gcggcgccgc agccccacat agatccagag
     1561 attcttgaac gcctcggcgc agacgatggt cacgaagatg agctgcacgt cgtgcagcca
     1621 gtacgtggcg gccaacaacg tcgcgatgcg ccacacggtc acggcgagcg tgtagcccat
     1681 gaccgccaca gactgcccat gcgccagcca gtacgacacc agcacttcca ggttgagaaa
     1741 caggaacacg tagagcgcga gcagcaccca cgcctgcgaa atctccggcg ggactatcca
     1801 ctgctggaac accacgatga tgagcgcgct cgcgacgctg acgccgagca tcagcaggca
     1861 ggtattgctg atatcgaccg cttgccgatc gggagaccgg gcgatcaggt aattgagatt
     1921 cgcgctcagt gcgaatccgg cgagcgacgt gatgaacatg gcggtagcca tgaactgccg
     1981 gtagcggccg tactcggaaa tttcgagcag gcgcaccagc aacagcggac tcagaagcgc
     2041 cagggcttga ttgaggaggc gcgcgctggc gagcacggcg cccctcgaga caaatgaact
     2101 catgcgttac gggattctgg cagtgcggtc aaacctcgca ctgtcggaca aggtaaggag
     2161 cggcctacaa cattggcggg agttgactcg ctcccggttc cggagcgttc ctagtcaaat
     2221 cgggccgcgg atagccgtta atcgcaccat gaaattcaaa accgcagagg cccgacttcg
     2281 agtgctgcac ccagttaccg cggaagccac gcagcttcct tcccgagacg acctcccggc
     2341 gattcccgcg atgtcacgca tgatcgaact catggagatg gcggcggcac ggctcatgag
     2401 gccgcgcctg cagaacggcg agtcgtccgt cgccgtggcg atgaacgtca cgtacgccgc
     2461 gccgcaaatg cgcctgcccg gtgcggcatc cacgttgcgc gcggtggcct cttacaagga
     2521 aatttccgga cggctgcatc gcttcacgat caacgcattc gacgaatccg ggctggttgg
     2581 cacggccgaa cacacgcgcg cggtgctggc ggagcggcgc ctctccgatg ttgcgcgtca
     2641 gcaggccggc aaaaccggcg agcggctgat cgcctgacgc tttctcacgc cagcagtacg
     2701 tagatgccga gcgccgcgaa cgtgagcgcg gctgccacgc gcacccattt cagtggcacg
     2761 cgtttgatca gcgcatcgcc caggtagatc accggcacgt tcgccagcat cat
//
LOCUS       DATDEU010000732         3303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_726283, whole genome shotgun sequence.
ACCESSION   DATDEU010000732 DATDEU010000000
VERSION     DATDEU010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726283"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VIV63_17445"
     CDS             complement(<1..1266)
                     /locus_tag="VIV63_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126443.1"
                     /translation="MKSAFACILLVGAASLASARPVVIEETTTLTNPNPTAYAQFGRM
                     VATNGEYALVLGSRDSVDEQYNEIQTRDALLYRRVAGQWQYQQVLRASQTFNEGYNFP
                     DLLAMNGNLAVLELDGNTQAYRLTGGAWQPAGEFRTLTEDIEIDGDRVVTTTGDCSWS
                     GRVREADGSGGWVATGLSGQPRGCDDEHWGGPADIAGDRVILGNAGTFDLEDQEAVIW
                     QRSGLGSWYRAGSIVSPFGHTGFSGEVALRGDDAIVDADLGAYVYRLSNLTQSVSRLR
                     SADTYLQSRISPFGTRKIEKSGELVFVRQPSYDRASQVINVFRANAAAPGTYDHVAVL
                     APRGGGPLINWFDVSGNTVIASGAETAYIFELPASLTSPAPRQDNFETGSAAGWTPIA
                     GSQFSVVTSGTNRIYRQSSTAGEARALLGN"
     gene            complement(1464..>3303)
                     /locus_tag="VIV63_17450"
     CDS             complement(1464..>3303)
                     /locus_tag="VIV63_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6126444.1"
                     /translation="SAAFPGNSVGAVVDYVTRMPEDLEMRARLTSFGQDFEVYGSADR
                     HTGWQASASVGDRSGNFSWWLNFNHLDSDGQPLSFANKLVSSGVPGNAGLPVTGAIPD
                     RNPREQDWLILGSTTQIHTVQDHGKAKLSYDFTPALRASYTLGVWNNDAQRNSDSYLR
                     DAAGNAVYSGNINVGGRSYAVTAADFAPARGDLTHLMHGLSIKSHGRDVWDWEAAASI
                     YDYVDDITRAPTVPLPGAGAGGAGRITDQSGTGWQALALRGTWRPQGVDGAHLVDVGL
                     QVDMNELQSLVSNTGNWLEGGATSRFSAFQGKTQLQSAYAQDSWRFAEDWRATLGVRV
                     EHWRAHDGAIANATSILTFDERSETHVSPKAALAWQVASAWVLKASIGRAVRMPTVSE
                     LYQGSISVDVIVNNDPDLKPEQSWTGELTAEREFGNGLLRTTYFHEDTRDALYSQTNV
                     TVIPNVTNIQNVDHVRTRGIELAYQANDVFMSGLDLSGSVTYAHSRTVENANFPASVG
                     KWQPRVPEWRANALANYRFGERWSATLGGRYSGRQYNTLDNSDPNAFSFTGTSSFLVF
                     DARVRCRIGERWIASVGVDNLGNEEYWAFHPYTQRTISAELSADFL"
BASE COUNT          556 a         1021 c         1122 g          604 t
ORIGIN      
        1 gttgccgagc agggcgcggg cttctccggc cgtgctgctc tggcgataaa tccgattcgt
       61 cccactggtg acgacggaga attgcgatcc cgcgatcggg gtccagcccg cggcacttcc
      121 cgtttcgaag ttgtcctggc gcggagccgg cgaggtcagc gatgccggca gctcgaagat
      181 gtaggcggtc tcggcgccgc tcgcgatcac ggtgttcccg ctcacgtcga accagttgat
      241 gagcggtccg ccgcctcgcg gcgcgagcac cgccacgtga tcgtaggtgc cgggcgcggc
      301 cgcattcgcg cgaaacacgt tgatgacctg cgacgcgcgg tcgtagctgg gttggcgaac
      361 gaataccagc tcgccgctct tttcgatctt gcgggtgccg aacggggata tgcgggactg
      421 tagataggtg tccgcggatc ggagccggct gaccgattgg gtcaggttcg agagccggta
      481 aacataggcg ccgaggtcgg cgtcgacgat ggcgtcatcg ccgcgcagcg caacctcgcc
      541 actgaaaccg gtgtgtccga acggagagac gatcgagccc gcgcgatacc aggaaccgag
      601 gccagaacgc tgccagatga cggcctcctg atcctcgaga tcgaaggtgc cagcattgcc
      661 gaggatcacg cggtcgccgg cgatgtcagc gggtccgccc cagtgttcgt cgtcgcagcc
      721 gcgcggctgt cccgaaagtc cggtggcgac ccacccaccg gagccatctg cttcccggac
      781 gcgcccgctc catgagcagt cgcccgtagt ggtgacgacc cggtcgccgt cgatttcgat
      841 gtcttcggtg agcgttcgaa actcgccagc cggctgccag gcgccgccgg tgagtcgata
      901 ggcctgcgtg ttgccgtcga gctcgagtac cgcaaggttg ccgttcatcg cgagcaggtc
      961 aggaaagttg tagccctcgt tgaaggtctg gctggcgcgc agcacctgtt ggtactgcca
     1021 ctgtcctgcg acgcgccggt agaggagggc atctctcgtt tgaatctcgt tgtactgctc
     1081 gtcgacggag tcccgcgaac ccaggaccag tgcgtattcg ccgttggtgg cgaccatgcg
     1141 gccgaattgc gcataggcgg tcgggttggg attggtcagc gtggtcgttt cttcgatgac
     1201 gacgggacgc gcggacgcga gactcgcggc acccacgagc aggatgcagg caaaagcgga
     1261 tttcatggtg ttccctcggg agcagccgcc ggaacggagc cgtatcccac ggtagttagt
     1321 atcccgcgcg cgccgggcgc ttcatgcacg acaagcacgc gagatgccgt cggcgggtga
     1381 cgacatcggg gaaaacccgg tcgaacgaaa acgcggcgct tcctacagcc gggacagggt
     1441 ttagttaggc gcttccgcct ttcctacagg aaatcggcgg acagttcggc ggagatggtt
     1501 cgctgggtgt aagggtggaa agcccagtac tcctcgttgc cgaggttgtc gacgccgacc
     1561 gacgcgatcc agcgctcacc gatccggcag cgcacgcgcg catcgaacac caggaagctg
     1621 ctcgtacccg tgaaggagaa ggcgttcggg tcggagttgt cgagcgtgtt gtattgccgg
     1681 ccgctgtacc gcccgcccag cgtcgccgac cagcgttcgc cgaaacgata gttggcgagc
     1741 gcgttggccc gccattccgg cacgcgcggc tgccacttgc cgacactggc cgggaagttg
     1801 gcgttctcga ccgtgcgcga gtgcgcgtag gtgacgctgc ccgagagatc gaggccgctc
     1861 atgaatacgt cgtttgcctg gtaggcgagt tcgatgccgc gcgtacgaac gtggtcgacg
     1921 ttctggatat tggtgacgtt gggaatgacc gtgacattgg tctgtgaata cagggcgtcg
     1981 cgcgtgtctt cgtgaaagta ggtggttcgc agcaatccgt tgccgaactc gcgttcggcc
     2041 gtgagttcgc ctgtccagga ttgctccggc ttcaggtcgg ggtcgttgtt gacgatgacg
     2101 tcgaccgaga tcgagccctg gtacagctcg gaaaccgtgg gcattcgcac cgcgcggccg
     2161 atcgaggcct tcaacaccca ggcggacgca acctgccagg ccagcgccgc cttcggcgac
     2221 acatgcgtct cgctacgttc atcgaaggtc aggatgctgg tcgcattggc aatggcgccg
     2281 tcgtgagcgc gccagtgctc gacgcgaacg cccagcgtgg cgcgccagtc ctcggcgaag
     2341 cgccagctat cctgtgcgta cgcgctttgt agctgcgtct tgccctgaaa agccgaaaag
     2401 cgcgaggtgg cgccgccttc caaccagttg ccggtattcg aaaccagcga ttgcagctca
     2461 ttcatgtcga cctgcaggcc gacatcgacg agatgcgcgc cgtcgacacc ttgcggacgc
     2521 caggtgccgc gcagcgccag cgcctgccag ccggtgccgc tctggtcagt aattcggccg
     2581 gctccgccgg cgcccgcgcc aggcaacggc accgtgggcg cgcgcgtgat gtcgtccacg
     2641 tagtcgtaaa tgctggcggc tgcttcccag tcccatacgt cgcggccatg ggatttgatc
     2701 gacagcccgt gcatgaggtg cgtgagatcg ccgcgggcgg gcgcgaagtc cgccgcggtt
     2761 accgcgtaac tgcgaccgcc gacgttgatg ttgccgctgt acaccgcgtt gccggcggcg
     2821 tcccgcagat aggaatccga gttccgttgg gcgtcgttgt tccacactcc cagcgtgtag
     2881 ctggcgcgca gcgcgggcgt gaagtcgtaa ctcagcttcg ccttgccgtg gtcctggacg
     2941 gtatgaatct gggtagtgga gcccaggatc agccagtcct gctcgcgcgg gttgcgatcg
     3001 ggaatagcgc cggtgacggg caagccggcg ttgccgggta caccgctact cacgagtttg
     3061 ttcgcgaaag agagcggctg cccatcgctg tccaggtggt tgaagttgag ccaccaggaa
     3121 aaattgccgc tgcgatcgcc gacggaagcg ctcgcctgcc atccggtatg acgatcggcc
     3181 gagccataaa cctcgaagtc ctggccgaag ctcgtgagcc tggcgcgcat ctccaggtct
     3241 tcgggcatgc gtgtgacgta atcgaccacc gcgccgaccg aattgccggg gaacgcggcg
     3301 gag
//
LOCUS       DATDEU010000733         3468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1309357, whole genome shotgun sequence.
ACCESSION   DATDEU010000733 DATDEU010000000
VERSION     DATDEU010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309357"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VIV63_17455"
     CDS             <1..427
                     /locus_tag="VIV63_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126445.1"
                     /translation="RNWLRALRKIRLFTETLAFGDERQAGIAPCEYARPAFDVLSTYA
                     GAKGGLGALSLAKKSRDCLLEATKLDPAALKGSLNPDGMDPNYFIGELLYEKGEYSRS
                     LQHLQKALAAPPRPDRPVADAGRRAEIDTLVAKVRTKLE"
     gene            complement(431..1786)
                     /gene="yjjJ"
                     /locus_tag="VIV63_17460"
     CDS             complement(431..1786)
                     /gene="yjjJ"
                     /locus_tag="VIV63_17460"
                     /inference="COORDINATES: protein motif:HMM:NF007297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HEY6126446.1"
                     /translation="MPARPAEYILRRLTEGATSSSELEQLLGQSQSTLSRLLRQLVAS
                     GQIIRIGSRRGARYALLRPVEGIGARWPLRRIDDRGQVHDLGTLHALSGGEYLMETAS
                     GEFHWSGVTPALPYYLQDQRPAGFLGRAVPRRYPELALPQRVMDWNDDHYLRYLTQRG
                     SDTVGDLVLGDRALDEALQLMQQRPVIRVAERGSSYPRLVEQVMEGGLPGSSAHGEHP
                     KFVTLLDGRPASQVLVKFSPSTRTRVGERWADLLIAEHHAHEVLRGEGIAAAHSRIER
                     FADRIYLETIRFDRVERAGRLGVTSLFAIDAALYGKLDNWIDAGKRLLADRRIDAATL
                     GQIRLLATFGALIANTDRHLGNLACFDRYDGRFTLAPVYDMLPMLYAPEHDELPARTF
                     VAPAPTSDSLREYGRARVLAEQYWTACAADARISEDFRGIAAGNLQSLRALPRTGAFT
                     A"
     gene            complement(1917..2615)
                     /locus_tag="VIV63_17465"
     CDS             complement(1917..2615)
                     /locus_tag="VIV63_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126447.1"
                     /translation="MHTRVVLLICALASSGLASAADVACPSTSGADLEPTFDATLLKE
                     GRFIYHTTLKGESLGETAIEVRRDGAHVVISMSAPKVAQSWKATVAQSFAPLSASLEM
                     QGRKGPYAMELKYAGASITGEEREGGVTSPVNASAEGVVLDQRVDWAAMMASTARTGS
                     SIVVRVFDPSTGMSEMLGRIGETQSALRLDYSICKRDHLEEYTVYATRETPRYMLRED
                     MPNGLVSELVRIEP"
     gene            complement(2677..>3468)
                     /locus_tag="VIV63_17470"
     CDS             complement(2677..>3468)
                     /locus_tag="VIV63_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126448.1"
                     /translation="LILLTPLALFALVYIGAAVMLALRDDRIAALEKPVATDKSIAIF
                     GASGTAGDGILKAALASPDIEKIHVVTRRSTPRIEQGVAAGKVQMTLHQDYLDYSAIT
                     DQIVAVDAVFWALGTSSLGVDEKTYGTIHVDYPASFVAKWLSVSGQPAISFHFISSSD
                     ISDDSTAMWAREKVRAERTLSNLAEGTKLRVVAYRPDYIGPTDEEAHFGQTLLYGFFA
                     PIGAAVKAEQIGKAMIEVTARNQFKNGDKFGTRSIIRYADAYDRR"
BASE COUNT          641 a         1095 c         1096 g          636 t
ORIGIN      
        1 gcgcaactgg ctgcgcgccc tcaggaagat cagattgttt accgagacac tcgcgttcgg
       61 cgacgagcga caggcaggca tcgccccgtg cgaatacgcc cgccctgctt tcgacgtgtt
      121 gagcacctac gcgggcgcga aaggcggcct gggtgcgttg tcgctggcaa agaagtcgcg
      181 cgactgcctg ctcgaggcca ccaagctgga tcctgccgcg ctcaaggggt cgctcaatcc
      241 ggacggcatg gaccccaact acttcatcgg cgaattgttg tacgagaaag gggagtactc
      301 ccgttcgctg caacatctcc agaaggcgtt ggcggcgccg ccacgtccgg accgaccggt
      361 cgccgatgcg gggcggcgcg cggagatcga cacgctcgtc gccaaggtgc ggacgaaact
      421 cgaatgagat tcaagccgtg aacgcgcccg tgcgcggcag ggcccgaagc gactgaaggt
      481 tgccggcggc gatgccgcga aaatcctcgc tgatgcgcgc gtcggcggcg caggcagtcc
      541 agtactgctc cgccagcacg cgagcgcgac cgtattcgcg caggctgtcg gaagtcggcg
      601 cgggcgcgac gaaggtgcgc gcgggcagtt cgtcgtgctc cggtgcgtag agcatgggca
      661 acatgtcgta cacgggcgcc agcgtgaaac ggccgtcgta ccgatcgaaa caggcgagat
      721 tgcccaggtg tcggtccgtg ttcgcgatga gcgcgccgaa agtcgcgagc aggcggatct
      781 ggcccagcgt tgcggcatcg atgcgccgat ccgccagcag ccgcttgccc gcgtctatcc
      841 agttgtcgag cttgccgtag agcgcggcgt cgattgcgaa cagagaggtg acgccgaggc
      901 gccccgcccg ctccacgcgg tcgaagcgga tcgtctcgag gtagatgcga tccgcgaacc
      961 gctcgatgcg tgaatgcgcc gcggcgatgc cctcgccgcg cagaacctcg tgcgcatggt
     1021 gctcggcgat gagcaaatcc gcccagcgtt cgcccacgcg tgtgcgcgtg gacggcgaga
     1081 acttcaccag cacctgcgac gccggccgac cgtccagcaa agtgacgaac ttcgggtgct
     1141 ctccgtgcgc cgatgaccct ggcagcccgc cttccatgac ttgttccacg aggcgtggat
     1201 aactcgagcc gcgctcggct acgcgaatca ccgggcgctg ttgcatgagt tgaagcgcct
     1261 catcgagcgc gcgatcgccg agcaccaggt caccgacggt gtccgaaccg cgctgcgtga
     1321 gataacgcag gtagtggtcg tcgttccagt ccatcacgcg ctgaggcaag gccagttccg
     1381 gataacgccg cggcaccgca cggcccagga accccgcggg gcgttgatcc tgcaggtagt
     1441 aaggcagcgc tggtgtcact ccactccagt ggaattcgcc tgacgccgtc tccatcagat
     1501 attcgccgcc cgagagtgcg tgcaacgtgc ccaggtcgtg cacctggccg cggtcgtcga
     1561 tacgccgcaa cggccaacgc gcgccgatgc cttcgaccgg ccgcaacagt gcgtaacgcg
     1621 cgccacgtcg gctgccaatg cggatgatct gtccgctggc gacgagttgg cgcaacagcc
     1681 gggaaagcgt ggactgcgat tgaccgagca actgctcgag ttcgctcgag gaggtagcgc
     1741 cttcggtcag gcgtcgcagg atgtattcgg cggggcgggc tggcatggcc gcaatgatac
     1801 tagttgaatg actaatttat tggcttattt atacctgtat tttttgttgt taaacaacat
     1861 attacagatt acttgaacga gaaaatgact aattatatcg acggaaccac tctatattac
     1921 ggctcgatac gaaccagctc ggataccagg ccgttgggca tgtcctcgcg cagcatgtat
     1981 cgcggggtct cgcgcgtggc gtacaccgtg tactcctcga gatggtcgcg tttgcagatc
     2041 gagtagtcga ggcgcagcgc ggattgcgtc tcaccgatcc ggcccagcat ctcgctcatg
     2101 cccgttgacg gatcaaagac ccgcacgacg atcgaactac ccgtgcgtgc ggtcgaagcc
     2161 atcatcgcgg cccagtccac gcgctgatcg agcaccacgc cttcggccga tgcgttcacg
     2221 gggcttgtga cgccgccctc gcgttcttcg cccgtgatgc tggcgccggc gtatttcaac
     2281 tccatcgcgt agggaccctt ccgcccctgc atctcgagcg aggccgagag tggcgcaaac
     2341 gattgcgcca ccgtggcttt ccaggattga gcgactttcg gcgcgctcat cgaaatgacg
     2401 acgtgcgcac cgtcacgccg aacctctata gccgtctcgc cgagcgactc gcctttgagc
     2461 gtcgtgtgat agatgaatcg cccttccttg agaagggtgg catcgaaggt gggttcgagg
     2521 tccgcgccgc tggtcgacgg acacgcgacg tcggcagcgc tcgccagacc actcgacgcg
     2581 agagcgcaga tcaacaacac gactcttgta tgcatgactg atgctagcaa gccaagctgc
     2641 gcgggagtcc tcgtccggtc gagtacgaag aacggctcaa cgcctgtcgt atgcgtccgc
     2701 gtagcgaatg atgctgcggg tgccgaattt gtcgccgttc ttgaattgat tccgcgcggt
     2761 gacttcgatc atcgccttgc cgatctgctc ggccttcacc gcggcgccga tgggcgcaaa
     2821 gaacccatat aaaagagtct ggccgaaatg cgcctcttca tcggtcggcc cgatgtagtc
     2881 cggccgatag gccactacgc gcagtttcgt gccttccgcg aggtttgaca gcgtccgttc
     2941 ggcgcgaact ttctcgcgcg cccacatcgc ggtcgaatcg tccgagatat cgctcgaact
     3001 gatgaagtga aacgagatgg cgggctgacc gctgacactg agccacttcg ccacgaagct
     3061 cgcgggatag tcgacgtgaa tcgtgccgta ggtcttttcg tcgacgccca gagagctggt
     3121 tcccagcgcc cagaacaccg catcgaccgc gacgatctgg tccgtgatcg ccgagtagtc
     3181 gaggtaatcc tggtgcagcg tcatctgcac ttttccggcc gcaacgccct gttcgattcg
     3241 cggcgtcgag cggcgggtga cgacatggat cttctcgatg tccggactcg ccagggcggc
     3301 cttgagaatc ccgtctcccg cagttccgct ggcgccaaag attgcaatcg acttgtccgt
     3361 cgctaccggc ttttcgagcg ccgcgatccg gtcatccctg agcgcgagca tcaccgccgc
     3421 accgatgtac accagcgcga acagcgccag tggtgtgagg aggatgag
//
LOCUS       DATDEU010000734         6992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1891995, whole genome shotgun sequence.
ACCESSION   DATDEU010000734 DATDEU010000000
VERSION     DATDEU010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891995"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VIV63_17475"
     CDS             complement(<1..283)
                     /locus_tag="VIV63_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126449.1"
                     /translation="MFYTHACRTLTAVLFALPIVSLADDSPSTEPVLTEAEQRQLGAG
                     WYPLGIQDGWASVSTTALPNGTTGGSNAAMERRVVVTNRAQLVAALAYPD"
     gene            458..1120
                     /locus_tag="VIV63_17480"
     CDS             458..1120
                     /locus_tag="VIV63_17480"
                     /inference="COORDINATES: protein motif:HMM:NF036878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY6126450.1"
                     /translation="MATNYVLVDFENVQPDSLAALATGQFRVKVFVGAAQAKGRISFE
                     LSHSIQTLGANAEYVKIARTGKNAVDMHIAYYVGRLLEKEPGAMIHIISKDADFDPLI
                     EYLNAKGSHCKRVKTIAEVPKLAASRAAAPAAKPVVKHVPHVPTPRRPHSEKLAPIIK
                     HLHSLSGKPATSKKLTQTIATYFKQHGGALGDKTVALILDELVRLKYVSQEGTKVSYH
                     LS"
     gene            1161..1799
                     /locus_tag="VIV63_17485"
     CDS             1161..1799
                     /locus_tag="VIV63_17485"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEY6126451.1"
                     /translation="MKITKLLAAGVLVSLPAMAQAQGWYAGIDVGAARSDAEIDEYAF
                     FGTATDRNSGDTTGIRLRGGFQFGRFFALELSYVDFGEFESHFDPDDCPNGAPGPCPV
                     DVRTSINGTVLTAVGIVPFGEHWFLNARAGYGKMKIETQAFGATDIDGSSSNDGFHFG
                     IGGGYRFNEHWNVLLDYSSYDQMDFGLTLSGAFGAYNQGETTMTSLGVNYSW"
     gene            1915..2658
                     /locus_tag="VIV63_17490"
     CDS             1915..2658
                     /locus_tag="VIV63_17490"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEY6126452.1"
                     /translation="MRFNFTAIVLCAVCIAPAAQAQWTGKAELGYLQSSGNAESTSAN
                     TKFDLKRESEKWINTFYVGALYGNTGEFTNAERYEARYQLDYKINDRLSWFGALRGEK
                     DKFSGFVYQATASTGVAYKFIDTPTTKLAFSVGAGYRQLQEEVLIRTDAGEVIDRIEG
                     EEQSDPVGTLGSTYEHNFTENTKITNKFLAESGSANTALQDDLALQVNMTKTLALAVG
                     YGIRYNTDPPPLSETTDTLITVNLVYNIL"
     gene            2655..3197
                     /locus_tag="VIV63_17495"
     CDS             2655..3197
                     /locus_tag="VIV63_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126453.1"
                     /translation="MKMQHTLLAGLSGAVMALFAAPAMPQAASGMGSSASISVASGPH
                     AGKYAFAPREACVIAAFSDQPLGLSVVLSSENSSLSLDMPNLDEKHANEIQIVLVVAD
                     KKAGASMKGTASTTYEIDTRPDASLKPFQKAERANKGKSGKASTQLMTQGSNVLLTFS
                     GETETGVKLEGEVTCRKVAS"
     gene            complement(3246..4307)
                     /locus_tag="VIV63_17500"
     CDS             complement(3246..4307)
                     /locus_tag="VIV63_17500"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6126454.1"
                     /translation="MSAIGWLAACGGGGEDAVAPPATPIPDPFIDVDRAASAAFSAQG
                     ISGMGLAIYDAQGVKRFEKMYGTFAANQRVAIASSSKMVAGLVLLRLVDQGFLTLDST
                     TGAVLGWTGPQGAITLRQLLSFTSGLEREAACTLLAGITLADCVTSISQQGLVAAPGT
                     RFDYGSTHLHVAARMAEVVTGQAWANIFATQLKAPLGLSSPDLAWFTAPRQALGTSNP
                     LIAGGLRATMSEYASLLALDFNRGVHQGNRLIGDALFSAQATEPYPDAVIGNSPFQNA
                     GINFHYGLAAWLECPPPATNCAVLSSPGAFGFTPWFDRDGGYYAILGMEVTDSQSGVV
                     AFAVNLAQELKPLIRTALR"
     gene            4501..5187
                     /gene="aqpZ"
                     /locus_tag="VIV63_17505"
     CDS             4501..5187
                     /gene="aqpZ"
                     /locus_tag="VIV63_17505"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEY6126455.1"
                     /translation="MPILKRSVAELLGTFWLVFGGCGSAVLAAKFPEVGIGLVGVSLA
                     FGLTVVTMAYAVGPITGGHFNPAVTLGMLVGGRIKSADVVPYIIAQVIGAILAAWVLA
                     TIASGNGTDAVAGGLASNGFGEHSPGGYSMGTAFVTEVLMTFFFLLIILGTTDPRANV
                     NFAPLAIGLALTLIHLISIPITNTSVNPARSTGPALIVGGWALQQVWLFWVAPILGAV
                     AAGGFWRWMK"
     gene            complement(5207..5452)
                     /locus_tag="VIV63_17510"
     CDS             complement(5207..5452)
                     /locus_tag="VIV63_17510"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6126456.1"
                     /translation="MTAELTFLPGQQLQPPHQHADEEFQYVIEGSGTWSLNGKDIPLK
                     AGDLMYSKPMDWHGIRNSGATPLRFFVFKWKTRSDAD"
     gene            5802..6215
                     /locus_tag="VIV63_17515"
     CDS             5802..6215
                     /locus_tag="VIV63_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126457.1"
                     /translation="MRIVHSLDELLELPREPGVNGYRAREPLRLEIPRLASDVSTRAQ
                     DSLNRLQERCGSLAAAGVMFLTLVAGVFKVFTRNPTLFSWQAFGELVAVLVVSFCLGA
                     IAKMIALAVTRWQFARRCREHYEQLSRLVQGAATR"
     gene            complement(6212..6757)
                     /locus_tag="VIV63_17520"
     CDS             complement(6212..6757)
                     /locus_tag="VIV63_17520"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEY6126458.1"
                     /translation="MRTESSEVNAIDNKIVIIRGQSVILDSDLAHLYCVTTKALLQGA
                     RRNRKRFPHDFMFQLSKQEVTNLRSQIVTSSSEQGYGGRRYSPWAFTEQGVAMLSSVL
                     RSDVAVRVNIEIMRAFVRLRRAAMASGQLMSLVEDLSKRVETHDAVISDIVESIRQLV
                     EPPVHGRARPIGFTADLDTKD"
BASE COUNT         1405 a         2255 c         2077 g         1255 t
ORIGIN      
        1 catcggggta tgccagcgcc gcgacgagct gcgcgcgatt ggtgacgacc acgcgtcgct
       61 ccatcgccgc attcgagccc ccggtcgtgc cgttgggcag ggcggtcgtc gagacggacg
      121 cccagccgtc ctggattccg agcgggtacc agccggcccc gagctgccgc tgctcggcct
      181 ccgttagaac cggttcagtg gaaggcgagt catccgccag cgagacaatg ggcaaagcga
      241 aaaggaccgc ggtgagcgtg cggcacgcgt gtgtgtaaaa catgtatttc ccccaattga
      301 cgaacgtttt cgtggctatc agcgcgttct gcgacgcgta tgacaccggt agacatcgtg
      361 cgccaattcc ggccaccgaa tcaagtgcca ttggcttgtc caggacatcg ccgcactcgt
      421 cctacattcg agtcggcaaa cgtgcacaat cgcgcccatg gcaactaact acgtactggt
      481 cgacttcgaa aacgttcaac ccgacagcct cgcggcgctg gccaccgggc agtttcgcgt
      541 gaaggtgttc gtcggcgccg cccaggccaa gggacgcatc tccttcgagc tgtcacattc
      601 catacagacg ctgggtgcga acgccgaata cgtgaagatc gcgcgcaccg gcaagaatgc
      661 cgtggacatg cacatcgcct attacgtcgg acgcctgctc gagaaagagc cgggcgcgat
      721 gatccacatc atctcgaagg acgccgactt cgatccgctc atcgaatacc tgaacgcgaa
      781 gggcagccat tgcaagcgcg tgaagaccat cgccgaagtg cccaagctcg cagcgtcgcg
      841 cgcggccgcg ccggcggcca agcccgtagt gaaacacgtt ccgcatgtgc ccaccccgcg
      901 ccggccgcat tcggaaaagc tcgctcccat catcaagcat ctgcattcgc tgagcggcaa
      961 gcccgccacg tcgaagaaac tcacgcagac gatcgcaacc tacttcaaac aacatggcgg
     1021 cgcgctcggc gacaagaccg tggcgttgat cctcgatgaa ctagtccggc tcaaatacgt
     1081 cagccaggaa ggcaccaagg tcagctatca tctgagctga ccagatcgat tcgactagtt
     1141 tgagtcaggg agagggactc atgaaaataa ccaaactact ggccgcgggc gtgctcgtga
     1201 gcctcccggc catggcgcag gcgcaaggct ggtacgcggg cattgacgtc ggcgccgcgc
     1261 gctcggacgc ggagatcgat gaatacgcgt tcttcggcac cgccaccgac cggaacagcg
     1321 gcgacaccac cgggatccgt ctgcgcggcg gcttccagtt cggtcgattc tttgcgctcg
     1381 aactttcgta cgtggatttc ggcgagttcg aaagtcactt cgatccggat gattgcccga
     1441 acggtgcgcc aggcccgtgt ccagtggacg tgcgcacgtc gatcaacgga acggtgctta
     1501 ccgcggtggg catcgtgcct ttcggcgaac actggttcct caatgcgcgc gccggctacg
     1561 gcaagatgaa gatcgagacg caggcgttcg gcgccaccga catcgacggc agcagcagca
     1621 acgacgggtt tcactttgga atcggcggcg gctaccgctt caacgagcac tggaacgtgc
     1681 tgctcgacta cagctcgtac gaccagatgg acttcggcct cacgctgagc ggtgccttcg
     1741 gcgcttacaa ccagggcgag accaccatga cgtcgctcgg cgtcaattac agctggtaat
     1801 gcggcagcgc ggaacgaaac tgtaagacat tcccgcaatc tctctttggg ttgaggcggc
     1861 gcccccgcac gggagataaa ctcgcgcgac taacaatgac agcgaggatg tcccgtgcga
     1921 ttcaatttca cagcgatcgt tctatgtgcc gtttgcatcg cacccgcggc acaggctcag
     1981 tggaccggaa aggcggaact tggctatctg cagtcgagcg gcaatgccga atcgacctct
     2041 gccaacacca aattcgacct caagcgtgag tcggaaaagt ggatcaacac gttctacgtg
     2101 ggcgcgctgt acggcaacac cggcgagttc accaacgccg aacgctacga agcgcgctat
     2161 cagctcgact acaagatcaa cgatcgcctg tcatggttcg gcgcgctgcg cggcgagaaa
     2221 gacaagttca gcggattcgt ctaccaggcc acggcttcca cgggcgtcgc gtacaagttc
     2281 atcgacaccc ccaccaccaa gctggccttc tcggtcggcg ccggttatcg ccagttgcag
     2341 gaagaagtgc tgatccgaac cgatgcgggt gaagtcatcg accgcatcga aggggaggaa
     2401 cagagcgatc cggtgggcac actcggctcg acgtacgaac acaacttcac cgagaacacg
     2461 aagatcacca acaagtttct cgcggagtcc ggctcggcga acaccgcgct gcaggacgat
     2521 ctggcgctgc aggtgaacat gaccaagacc ctggcgctgg ccgtgggcta tggaattcgc
     2581 tacaacacgg atccgccgcc gctgtccgaa accaccgata ccttgatcac cgtcaacctg
     2641 gtctacaaca tcctgtgaag atgcagcaca cattgttagc gggtttgtcg ggcgcggtga
     2701 tggcgctctt cgcggcgccg gcgatgccgc aagcggccag cggcatgggc agcagcgcca
     2761 gcatcagcgt ggcgtcgggt ccgcatgccg gcaaatacgc gttcgcgccg cgcgaagcct
     2821 gtgtcatcgc cgcgttcagc gatcagccgc tggggttgtc cgtggtgttg tcgtcggaga
     2881 attcgtcgtt gtcgctcgac atgcccaacc tcgacgagaa acacgccaac gaaatccaga
     2941 tcgtgctcgt ggtggccgac aagaaggcgg gcgccagcat gaagggcacc gccagcacca
     3001 cgtatgaaat cgacacgcgt cccgacgcgt cgttgaagcc gtttcaaaag gccgagcgcg
     3061 ccaacaaggg caagagcggc aaggccagca cccagctcat gacccagggc agcaacgtgt
     3121 tgctgacctt cagcggcgag accgagaccg gcgtgaagct cgagggcgaa gtcacctgcc
     3181 gcaaggtcgc gtcgtaatgg ggacattcct gatttgctag caaatgggga cgcgcctcga
     3241 ccggactatc gaagcgcagt gcgaatcagc ggcttcaatt cctgcgcgag attcaccgca
     3301 aacgccacga ctcccgactg cgagtcggtg acctccatgc ccagaatggc gtaataaccg
     3361 ccatcgcgat cgaaccacgg cgtgaatccg aaagcgcccg gcgacgacaa caccgcacag
     3421 ttggtcgccg gcggcgggca ctcgagccag gccgcgaggc cgtagtgaaa gttgatgccg
     3481 gcattctgaa acggcgagtt gccgatcacc gcatccggat acggctccgt cgcctgcgcc
     3541 gagaacaacg cgtcgccgat gagccgattg ccctgatgta cgccgcgatt gaaatcgagc
     3601 gccagcaacg atgcatattc gctcatcgtc gcgcgcagac cgccggcaat caacggattc
     3661 gaagtgccca acgcctggcg gggcgccgtg aaccacgcga ggtccggtga actcaacccc
     3721 agcggcgcct tcaactgcgt cgcgaagata ttcgcccacg cctgacccgt gacgacttcc
     3781 gccatgcgcg ccgccacgtg caggtgtgtg ctgccgtagt cgaagcgcgt gcccggcgct
     3841 gcgaccagcc cctgctgtga aatcgacgtc acgcaatccg cgagtgtgat gcctgccagc
     3901 aaagtacagg ccgcctcacg ctcgaggccc gacgtgaaag acaacaactg acgcaacgtg
     3961 atcgcgccct gcggccctgt ccagccgagc accgcgcccg tggtcgagtc cagcgtgagg
     4021 aaaccctggt cgacgagccg cagcaagacc agcccggcca ccatcttcga cgacgaggca
     4081 atcgccacac gctggttcgc cgcgaacgtg ccgtacatct tctcgaaccg cttcacaccc
     4141 tgggcgtcgt agatagccag acccattccg gagattccct gcgcggaaaa ggccgcgctc
     4201 gccgcgcggt cgacgtcgat gaaaggatcg gggatgggtg tcgcgggagg ggcgacagca
     4261 tcttcaccgc cgccgccaca cgcggcgagc cagccaatgg cggacatagc gagcacagtg
     4321 cgaaggaaac gcatgcgggt aacaacgcgc cttcgcgcct tccgttgact ccggggagac
     4381 ccctgaaaac cgcctggatt gcgaaaacgg ccaaccggcg accccttggg cctatttact
     4441 cttatatatg tcttgcttaa ggtgcgtcgc ccgacaagaa caacccaggg gagattttcc
     4501 atgcccattc tcaagcgttc cgtcgccgag ttgttaggta ccttctggct cgtgttcggc
     4561 ggctgcggca gcgccgtact cgccgcgaag tttccggaag tcggcatcgg tctcgtcggc
     4621 gtgtcgctgg cgttcggcct gaccgtcgtc accatggctt acgccgtggg tccgatcacc
     4681 ggtgggcatt tcaatcccgc cgtcacgttg gggatgctgg tgggcggacg catcaagtcc
     4741 gcggacgtag tgccttacat catcgcgcag gtcatcggcg ccattctcgc cgcatgggta
     4801 ctagctacca tcgcctcggg taacggcacc gatgccgtcg ccggcgggct cgcctccaac
     4861 ggattcggcg aacactcgcc cggcggttat tcgatgggca ccgccttcgt caccgaagtg
     4921 ctcatgacgt tcttcttcct gctgatcatc ctcggcacca ccgacccgcg cgcgaacgtc
     4981 aatttcgcgc cgcttgcgat tggactcgcg ctcacgctga tccatctcat cagcattccc
     5041 atcaccaaca cctcggtgaa tccggcgcgc agcaccggcc ccgcgctcat cgtcggtggc
     5101 tgggcgctgc agcaggtctg gctgttctgg gtcgcgccga ttctcggtgc cgtggcggcc
     5161 ggcggattct ggcgctggat gaagtgagaa acggtgcctg gcaccgttaa tccgcgtcgc
     5221 tgcgcgtctt ccacttgaac acgaagaagc gcagcggcgt ggcgcccgaa ttgcggatgc
     5281 cgtgccagtc catcggcttc gaatacatga ggtcgcccgc cttcaacggg atgtcctttc
     5341 cattgagcga ccacgttccc gatccctcga tcacgtactg aaactcttcg tcggcgtgct
     5401 gatgcggcgg ctgaagctgc tggcccggca ggaacgtgag ctcggccgtc aaaaccgaat
     5461 gcgtccccac cgattgctgc ccctctcccg tgtagacgga gatcgagccc cacgatcctt
     5521 tcgagactct cgcgtcgcta gctgaccaga tttgactgtc cagaaccggt ttcccgggct
     5581 tcgctgccca aagcgtgccg gcgaagaatg agccgccagc gagggcggcg gcgcacagaa
     5641 gtgttcgtag tttcatgcgg cgaaggtacc gtgccgacgc cccccgggca ccatttcccg
     5701 ggaattcgtc acacaaccgg acattccagc cgaaccctga agcacctgct gcggtgcgcc
     5761 cgggctaggg tgcagccaac acagggacta aggacagtca catgcgcatc gttcattccc
     5821 tcgacgaact gctcgaacta ccgcgcgaac ctggcgtcaa tggctatcgt gcgcgcgagc
     5881 cgctgcgcct cgagatcccc aggctcgcgt ccgacgtcag cacgcgcgca caggattctc
     5941 tgaaccgatt gcaggaacgc tgcggcagtc tcgcggccgc gggcgtgatg ttcctgacgc
     6001 tcgtcgccgg cgtgttcaag gtgttcacgc gtaatccaac gctcttctcg tggcaggcgt
     6061 ttggcgaact cgtcgccgtg ctcgtcgtgt cgttctgcct cggcgccatc gccaagatga
     6121 tcgcgctcgc cgtgacacgc tggcagttcg cgcggcgctg cagggaacac tacgagcagc
     6181 tgtcgcggct cgtgcaggga gcggcgactc gctagtcctt agtgtcgagg tcggcggtga
     6241 aaccgatcgg gcgtgcacga ccgtgcacgg gcggctcaac gagctgtcga atggattcga
     6301 cgatgtcgga gatgacggcg tcgtgcgttt cgacgcgctt ggacagatcc tccaccagtg
     6361 acatcaattg accgctggcc attgcggcgc gacgcaatcg aacgaaagct cgcatgatct
     6421 cgatgttcac gcgcaccgcg acgtcgctac gcagaacgct cgagagcatc gccactccct
     6481 gttcggtgaa agcccagggg gaatatcgcc ttccgccgta cccttgctct gaacttgagg
     6541 taacaatttg tgacctcaag tttgtaacct cttgtttcga aagctgaaac atgaagtcgt
     6601 gcgggaaccg ttttcggttt cggcgcgcgc cttgaagtaa tgccttcgta gtcacgcaat
     6661 aaagatgagc gagatcggaa tccaaaatta cgctctgccc ccgaatgatc acaattttgt
     6721 tgtcgatggc gttgacttcc gaagactcgg tgcgcatgcc gccaagtttc cttcgagcgc
     6781 gcctcttaga aaaccttcat tcttgtttcg ttaatgtaag aacacgatca ataaactacc
     6841 cccagatgct caacgaatcc gcctcctggc ctctccgccc cgtgcacggc ggcaagccgc
     6901 tgcccagcga ccacgtcgag ttcgctttcg aagacccgtc ctcacccgtg cacgaccgcg
     6961 tcatcaccga cgtcttccac gcacgcgtgt tc
//
LOCUS       DATDEU010000735         4510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1601177, whole genome shotgun sequence.
ACCESSION   DATDEU010000735 DATDEU010000000
VERSION     DATDEU010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601177"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VIV63_17525"
     CDS             <1..211
                     /locus_tag="VIV63_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126459.1"
                     /translation="ERARQAHVDAVVLWLIEEDEALPWEIARQMRSLADARIPALLLS
                     RQSWRAGETALNQVKLFVETLQVIS"
     gene            208..2391
                     /locus_tag="VIV63_17530"
     CDS             208..2391
                     /locus_tag="VIV63_17530"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY6126460.1"
                     /translation="MKVLEGLRVLAWRTGAESALARFLRDLGATVDRCDSALDATRLA
                     SSDLLIEQVGLTRIAETGLSRKDIDAANPKLVHVSVTTFGSSGPRARWRGSELVASAT
                     SGILRLVGDPDRPPVKEALDACGFHADMVAACGALSALYELLDSGKGQHVDVSVQEVA
                     FSRNVNGVLVWQFDRRLLQRAGGAVNYGRVAVRCIWPLADGYCFHSLMTGRFGAPANQ
                     ALSDWMDEAGADNPLRGVDWLRYNRSTLDPATRVSWESAIEKFFGTRTRHELNTEGRR
                     RGINATVVAAPADALDDPHLRARGFWSDSNGERQPGRFVSISGGHAAATLPAASRSST
                     PSVSMSHGPLAGVRVLDFSWALVGSITTKTLGDLGCEVIKVESRSRPCLSRLDVQVNA
                     SRADSFDDKPWFAHLNTSKLSLSLDMKHPASREVLDPLVDWADVVVENFSPGTMEKLG
                     LDYPRLAARNPRLVMASGSVFGQTGPLAQSWGVDGTGAALSGRTLLTGWPDRSPVIPS
                     AAPYGDVIVPYAMAASVIASLMQRRRSGRGCHIDASMYELCVQQMRGAFAGGGTSAAR
                     LGNADPAVFHQGVYATQGSDRWIAVTFESVAEWAAFTRAQGLRADDPVKREAELRRWC
                     ASRTDVDAVKLFQDLGVAAGVVQDMEDLFDRDPQIQERNSLTPFVHPILGTFGHVRTP
                     IDFSVSRPRPYRAPSMGEHSRQIAAALCGLSAQRIAELEELGVFK"
     gene            2388..3659
                     /locus_tag="VIV63_17535"
     CDS             2388..3659
                     /locus_tag="VIV63_17535"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEY6126461.1"
                     /translation="MSRSRKDLACTAAATAHQREFVADLKRRVVDNGEPFAIAQADTP
                     HEIFHAMDIPLISNQWWSAYISAKQLSPRYFAVLDKLGFPSNSCRYCSLGLACTLDKD
                     PSTAPWGGLPKPTVLVARLTCECIRHVFGQWAEAMGTDFFPLEAPGWTHKDPEWFDKS
                     NRDWEELYEPRRIELLVQEMRALIELLEARTGRRFDEQKFIALMHAVNEQEGYIAEAA
                     RLIGEARPCPVSIADQMPNTMIPQWHRGSPWAVAHARRFRDEVAERVASGAAASSNER
                     VRLMWIGAGLWHDPGFYSALEERHGAVFVWSMYMPFAGPAYIRELRDRPMHALASRVC
                     SMNEVLHLPPWMNSWMVSEAKRCGIDAAVVLVPPDNRLSQSGTLLTQHALESAGVPAL
                     RLSADMVDAKEWHHEDKVEYVADFLRSKGLI"
     gene            3656..>4510
                     /gene="dctP"
                     /locus_tag="VIV63_17540"
     CDS             3656..>4510
                     /gene="dctP"
                     /locus_tag="VIV63_17540"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY6126462.1"
                     /translation="MKSAAIALLATLLAGCAPQTTPGITVLTYASPYGPAHPFSRADI
                     TWAKWVAERSNGTLRIQPYWSGGVLSAEHSMTELRHGVVDIGLITPIYVRGGTHMIRV
                     QAAFYGGLTTFRQQVDLYHCMQKHDPQFAHELQGLVVLAVQGGNLPGILTRNRRVQTL
                     EDLAGLRLRAPAELLAVLKHFGADPVEMPMGEVYSALAKGVLDGVVAPKDTLKSLHFA
                     EVAHFFNTLSIPRGAYAARAMGERRWRTLDDGQRAILQDGVAVWERALESELRAARNA
                     QRVEEVRHL"
BASE COUNT          760 a         1489 c         1485 g          776 t
ORIGIN      
        1 cgaacgcgcg cgccaggcgc acgtcgatgc ggtggtgctc tggctgatcg aagaggacga
       61 agcgttgccc tgggaaatcg cgcgccagat gcgcagcctc gcggacgcgc gcatccccgc
      121 actgctgctg tcacgtcaat cgtggcgagc cggcgagacc gcattgaacc aggtgaaact
      181 tttcgtggag acattgcagg tgatttcgtg aaggtgctcg aaggactgcg cgtgctcgcg
      241 tggcggaccg gggccgaaag cgcgctcgcg cgtttcctgc gcgacctcgg cgccacggtg
      301 gatcgctgtg acagcgccct cgacgcaacg cgtctcgctt catcggacct gctgatcgaa
      361 caagtcgggc tcacgcgcat cgcggagacc gggctttcgc gcaaggacat cgacgcggcg
      421 aatccgaagc tcgtacacgt ttccgtgacg acattcggca gctcgggtcc gcgcgcgcgc
      481 tggcgcggtt cggaactggt ggcgtcggcg acgtcgggaa tcctgcgcct cgtcggcgac
      541 cccgaccgcc cgccggtcaa ggaggccctg gacgcgtgcg gtttccacgc cgacatggtc
      601 gccgcctgcg gcgcgctgtc cgcgttgtac gaactgctcg acagcggcaa aggtcagcac
      661 gtagacgtct cggtccagga agtcgcgttc tcacgcaatg tgaacggggt gctggtgtgg
      721 caattcgacc ggcgactgtt gcagcgcgcg ggcggcgcgg tgaattacgg acgcgtggcc
      781 gtgcgctgca tctggccgct ggcggacggt tactgcttcc actcgctgat gacggggcgc
      841 ttcggcgctc cggcgaacca ggcgctgagc gactggatgg acgaagccgg cgccgacaat
      901 ccgctgcgcg gagtagattg gctgcgatac aaccgctcga cgctcgatcc ggcgactcgc
      961 gtgtcatggg aatcagcgat cgaaaagttt ttcggcaccc gcacgcgaca tgagctcaac
     1021 acggaaggcc gtcgacgcgg catcaatgcg accgtggtag ccgcgcctgc cgacgcgctc
     1081 gacgatcccc acctgcgcgc gcgaggattc tggtcggatt ccaacggcga aaggcagccc
     1141 gggcggtttg tctccatctc cggtggccac gcggcggcga cgctgcccgc ggccagcagg
     1201 tcctccacgc cttccgtgtc gatgagccat ggcccgctcg cgggcgtacg cgtactcgat
     1261 ttttcctggg cactcgtggg atcgatcacc accaagacgc tgggcgatct cggctgcgag
     1321 gtcatcaagg tcgaatcgcg ctcgcggccc tgcctttcgc ggctcgacgt tcaggtcaac
     1381 gcatcgcgcg ccgacagctt cgatgacaaa ccgtggttcg cgcatctcaa cacgtccaag
     1441 ctgagcctct cgctggacat gaaacatccc gcaagccgcg aggtgctgga tcccctcgtc
     1501 gattgggccg acgtcgtcgt ggaaaacttt tcgcccggga ccatggagaa gttaggcctc
     1561 gattatcccc gcctggctgc ccgcaatccg cggctggtca tggcctcggg cagcgtgttc
     1621 ggacagacgg gtccgctcgc acaaagctgg ggcgtcgacg gtaccggtgc ggcgctgagc
     1681 ggccgcacgc tgctcacggg ctggccggac cgctcgccgg tgattccgag cgccgcgccc
     1741 tatggagatg tgatcgtgcc ctacgccatg gccgcgagcg tcatcgcctc gctcatgcag
     1801 cgacgacgca gcggccgcgg ttgccacatc gacgcctcca tgtacgagct gtgcgtgcag
     1861 cagatgcgcg gcgcctttgc gggcggcggt acctccgcgg ctcgccttgg caatgccgac
     1921 cccgccgtgt tccatcaagg cgtgtacgcg acgcaaggat cggatcgctg gatcgccgtg
     1981 acattcgaga gcgtggcgga gtgggccgca ttcacgcgcg cgcaaggttt gcgggccgat
     2041 gatcccgtga agcgcgaggc ggaactccgc cgatggtgcg catcacgaac cgacgtcgac
     2101 gccgtgaagc tttttcagga tctgggcgtc gccgccggag tggtgcagga catggaagat
     2161 ctgttcgatc gcgatccgca gattcaggaa cgcaattcgc tgacgccttt cgttcatccg
     2221 atcctcggga cgttcggcca cgtgcgcact cccatcgatt tctcggtttc gcgcccgcgg
     2281 ccctaccgcg cgccctcgat gggtgagcac agccggcaaa ttgccgctgc gctgtgcggg
     2341 ttgtccgccc agcggatcgc cgagctcgaa gaactcggag tattcaaatg agtcgcagcc
     2401 gcaaggatct ggcctgcacg gcggccgcga cggcccacca acgcgaattc gtcgcggacc
     2461 tcaaacggcg tgtcgtcgac aacggcgaac ctttcgccat cgcgcaggcg gacacgccgc
     2521 acgaaatttt ccacgccatg gacatcccgc tgatcagcaa ccagtggtgg tcggcgtata
     2581 tctccgccaa gcagttgtcg ccgcgttatt tcgcggtgct cgacaagctg ggcttcccct
     2641 cgaatagctg ccgctactgc tcgctcggcc tggcgtgcac gctcgacaag gacccatcga
     2701 ctgcaccgtg gggcggcctt ccgaaaccca cggtactggt cgcccgactc acctgcgaat
     2761 gcattcgtca cgtgttcggc cagtgggctg aagcgatggg cacggacttc tttccgctcg
     2821 aggcgcccgg ctggacgcac aaggatccgg agtggttcga caagtcgaac cgggactggg
     2881 aagaactcta cgaaccgcgc cgcatcgaat tgctcgtcca ggaaatgcgc gcgctcatcg
     2941 aactgctcga ggcgcgcacc ggccgtcgct tcgacgaaca gaagttcatc gcgttgatgc
     3001 acgcggtcaa cgagcaggaa ggttatatcg ccgaagccgc ccgcctcatc ggcgaggcgc
     3061 gcccctgccc cgtgtcgatc gccgaccaga tgcccaacac gatgatcccg cagtggcata
     3121 gaggttcgcc ctgggcggtc gctcacgcgc gtcggttccg cgatgaagtg gccgagcgcg
     3181 tcgcgagcgg cgccgccgcc agcagcaatg aacgcgtgcg cctcatgtgg atcggtgccg
     3241 gtctgtggca tgacccgggt ttctacagcg cgctcgagga acgtcacggc gccgtgttcg
     3301 tgtggtcgat gtacatgccc ttcgcgggac cggcctacat ccgcgagctg cgcgaccggc
     3361 ccatgcatgc attggcgagc cgcgtgtgct cgatgaacga agtgctgcac ttgccgccgt
     3421 ggatgaacag ctggatggtg agtgaagcga aacgctgcgg catcgacgcc gcggtcgtgc
     3481 tcgtccctcc tgacaaccgg ctgtctcagt cgggcacgct gctgacgcag catgcgctcg
     3541 aaagcgcggg cgtgccggcg ctgcgcctgt ccgccgacat ggtcgacgcc aaggagtggc
     3601 accacgagga caaggtcgag tacgtcgcgg atttccttcg atcgaaaggt ctcatatgaa
     3661 aagcgcagcc atcgcattgc tggccacgct gctcgccgga tgcgcgccgc agacgacacc
     3721 gggcatcacc gtactcacgt acgcatcgcc gtacggcccc gcgcacccgt tcagccgcgc
     3781 cgacatcacg tgggcgaagt gggtggccga acgctccaac ggcactctgc gcattcagcc
     3841 ctactggtcc ggcggcgtgt tgtccgccga acactccatg accgagttgc gtcatggcgt
     3901 ggtcgacatc ggcctgatca cgcctatcta tgtccgcggc ggcacgcaca tgatccgcgt
     3961 ccaggccgcc ttctacggtg gcttgaccac gttccgccag caggtcgacc tctatcactg
     4021 catgcagaag cacgatccgc agttcgcgca tgagctccag gggctggtgg tgctggcggt
     4081 gcaaggcgga aatctcccgg gcattctcac gcgcaaccgg cgagtgcaga cactcgagga
     4141 cctcgcaggg ctccgcctgc gcgcacccgc ggaattgctg gccgtcctga aacacttcgg
     4201 cgccgatccg gtggaaatgc ccatgggcga ggtgtactcg gcgctcgcca agggcgtgct
     4261 cgacggcgtg gtggcgccga aggacacgct gaaatccctg catttcgccg aggtggcgca
     4321 cttcttcaac acgctgagca ttccgcgcgg cgcgtacgcc gctcgcgcga tgggcgaacg
     4381 gcgatggcgc acgctcgacg acgggcaacg cgcgatcctg caggatggcg tggcggtctg
     4441 ggagcgagcg ctcgaatccg agctacgcgc cgcgcggaat gctcagcgtg ttgaagaagt
     4501 gcgccacctc
//
LOCUS       DATDEU010000736         2422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1456301, whole genome shotgun sequence.
ACCESSION   DATDEU010000736 DATDEU010000000
VERSION     DATDEU010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456301"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VIV63_17545"
     CDS             <1..711
                     /locus_tag="VIV63_17545"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY6126463.1"
                     /translation="WQWRIPTQQRTGNGHIYCREFINDDEATALLLANLDGEALAEPR
                     QIRFTAGHRQTFWSKNCIAIGLAAGFLEPLESTSIYLIQEGISRFISLFPDATLPDVV
                     RDEYNRHMRTEFEQVRDFIVLHFSATERDDTPFWNYCRTMSIPDTLRHKIELFRTAAR
                     VFRYEEELFSKPSWVAVLLGQNIVPKQCDPIVASLPHAMVHESLESMRLAMDEAASAM
                     PTHAEFIRRYCSSGVGAS"
     gene            708..2219
                     /locus_tag="VIV63_17550"
     CDS             708..2219
                     /locus_tag="VIV63_17550"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY6126464.1"
                     /translation="MSDLRIRDIVIVGGGTAGWMAAACFARLLKSPDIRIQVIESDAI
                     GSVGVGEATIPHILYFNRLLGLDEDAFVRTTNATFKLGIEFVNWGRVGQRYIHPFGPY
                     GVNMEGLHFHHFWLRHSRSADAPGVDAYNLQVLAAQAGRFQRPEMKMQGSPLQTISYA
                     FHFDASLYAKFLRDFALARGVTRIEGKITRVQQHAESGFIESVTLESGQTIPGDLFID
                     CSGFRGLLTEQTLQSGYDDWGSYLPCDRAIARACRKVGDPIPYTRATAKSAGWQWRIP
                     LQSRTGNGHVYCSEYLSDEAALESLNSDLDGETISEPNFLRFRAGIRRKPWNKNVIAL
                     GLASGFLEPLESTSIHLIQSAIARLMTNFPDKSFNQPDIDYYNRRTRLEFEQVRDFIV
                     LHYHATARDDSPFWDYCRNMPIPDSLAERIAIYRENARLYRHDNELFSETSWFAVMHG
                     QNIVPQRYHPVADILPQPELETRLAKMVEVTRNCRDTMPTHQQFIDSHCKAEV"
     gene            2216..>2422
                     /locus_tag="VIV63_17555"
     CDS             2216..>2422
                     /locus_tag="VIV63_17555"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY6126465.1"
                     /translation="MKVAEWHDVTAAKFTQEILPSGQPAVLRGLVRHWPSVQAGRTSP
                     AEIANYLQKFSTGANVRLMTADPSI"
BASE COUNT          467 a          772 c          728 g          455 t
ORIGIN      
        1 tggcagtggc ggatcccgac gcagcagcgc acgggcaatg ggcacatcta ttgccgggaa
       61 tttattaacg acgatgaggc gacggcgctg ctgctcgcca acctggacgg cgaggctctg
      121 gccgagccgc gccagatccg gttcaccgcg ggccaccgtc agacattctg gtcgaagaat
      181 tgcatcgcca ttggtcttgc tgcgggattt ctcgagccgc tcgaatcgac gtccatctac
      241 ttgatccagg aaggcatcag ccgcttcatc tcgctgttcc cggatgccac gctcccggac
      301 gtggtccgcg acgaatacaa ccggcacatg cgcacggagt tcgagcaggt gcgcgacttc
      361 atcgtcctgc atttctccgc gaccgagcgc gatgacaccc cgttctggaa ttactgccgc
      421 acgatgagca ttcccgacac gctgcggcac aagatcgaac tgtttcgcac ggcggcgcgc
      481 gtgtttcgct acgaagagga attgttctcg aagcccagct gggttgcggt gctcctgggg
      541 cagaacatcg tgccgaaaca atgtgacccg atcgtcgctt cgttgccgca tgccatggtg
      601 cacgaaagtc tcgagtcgat gcggctggcg atggacgagg cggccagtgc catgccaacg
      661 catgcggagt tcatccgccg gtattgttcg tccggagtgg gtgcgtcgtg agcgatctgc
      721 gcatacgcga catcgtgatc gtcggcggcg gcacggccgg gtggatggcg gccgcctgtt
      781 tcgcgcgact cctcaagagt cctgacatcc ggatccaggt gatcgagtcg gatgcgatcg
      841 gcagcgttgg cgtgggtgag gccaccatcc cgcacattct ttacttcaac cgcctgctgg
      901 gcctcgatga agacgccttc gtgcgtacga ccaatgcgac gttcaagctc ggaatcgaat
      961 tcgtgaactg gggcagggta ggccagcggt atatccaccc tttcggtccc tacggcgtca
     1021 acatggaagg tctgcacttc catcatttct ggctgcgcca ttcgcggtcc gcagacgctc
     1081 caggcgtcga cgcctacaac ctgcaggtgc tcgcggcgca ggcggggcgg tttcagcggc
     1141 ccgagatgaa gatgcagggt tcgccgctgc agaccatctc ctacgcgttc cacttcgatg
     1201 cttcgctcta tgcgaagttt ctgcgcgact tcgcgctggc gcgcggcgtg acccgcatcg
     1261 agggcaagat cacgcgcgtg caacaacacg cggaatccgg cttcatcgaa agcgtgacgc
     1321 tcgagagcgg tcagaccatc ccgggggacc tcttcatcga ctgctcgggc tttcgcggct
     1381 tgctcaccga gcagaccttg cagagcggct acgacgactg gggcagctat ctaccttgcg
     1441 accgggcgat tgcgcgcgcc tgccggaagg tgggtgaccc gatcccctac acgcgcgcca
     1501 cggccaagag cgccggatgg caatggcgaa taccgcttca gtcgcgaacc ggcaatgggc
     1561 acgtgtactg cagcgagtac ctctccgacg aagcggcgct cgagagcctc aattcggacc
     1621 tcgacggcga gacgatctcg gagccgaatt tcctgcgttt ccgcgccggc atccgcagga
     1681 agccctggaa caagaacgtg atcgcactgg gtctcgcttc gggcttcctc gaaccgctcg
     1741 agtcgacatc catccatctc atccagagtg ccatcgcgcg cttgatgacc aattttccgg
     1801 acaagtcctt caatcagccc gacatcgact actacaaccg gcgcactcgt ctcgagttcg
     1861 aacaagtgcg ggatttcatc gtcttgcact accatgcaac cgcgcgcgac gattctccgt
     1921 tctgggacta ttgcaggaac atgccgatcc cggactcact cgcggagcga atagctatct
     1981 accgcgagaa cgcgcgtctg tatcgccacg acaacgaact gttcagcgag accagctggt
     2041 tcgcggtgat gcacggacag aacatcgtcc cgcagcgata ccatccggtc gcggacatcc
     2101 tgccgcagcc cgagctcgag acacgcttgg ccaagatggt cgaagtgacc cggaattgcc
     2161 gggacaccat gcctacgcac cagcagttca tcgatagtca ttgcaaggcc gaggtatgaa
     2221 agtcgccgag tggcacgacg tgaccgccgc gaagttcacg caggagatcc ttccatcggg
     2281 acaaccggcc gtgctgcggg gcctggtacg ccactggccg tcggttcagg ccgggcggac
     2341 ctcgccggcc gagatcgcca actacctgca gaaattctca accggcgcga acgtcaggct
     2401 catgaccgca gatccgtcca tc
//
LOCUS       DATDEU010000737         5828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1309515, whole genome shotgun sequence.
ACCESSION   DATDEU010000737 DATDEU010000000
VERSION     DATDEU010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309515"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VIV63_17560"
     CDS             <1..434
                     /locus_tag="VIV63_17560"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6126466.1"
                     /translation="LCREELFPVASPQLFRGGCVPDTLAELAAYPLLHDTDAGNDQPW
                     LGWRGWFERAGLSTANVGRGPQFSDSIVLIGAAVAGLGIAIGRAPHVAPMLSRGQLVR
                     VTQETWMAPWNYYLMAPAAHFRRPLVRTFVDWALEEARSGQ"
     gene            503..1390
                     /locus_tag="VIV63_17565"
     CDS             503..1390
                     /locus_tag="VIV63_17565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6126467.1"
                     /translation="MSKRGWILFLSLGLLWGMPYLLIRIAVGEFDPLMVAVARTFLGS
                     LLLLPIALHRKALLPAFRKWKWLLAFTLIEISIPWVLLGHAETRINSSTAGLLIAVVP
                     LFTAIIVTRLGHEKLEPRRMFGLGLGFAGVALLVGLDIHFDDLPAVAATIAVALFYAI
                     GPIIIDRKLSDVPAIGVITASLMLGTLIYAPFVPFLWPETFSAPAVASVIGLGVLCTA
                     AAFMVFFALIAEVGPARATVITYVNPAVAILLGALVLSEPLTLGMAIGFPLVIAGSFL
                     GTQRSRTPVAATPVAESRA"
     gene            complement(1379..1858)
                     /locus_tag="VIV63_17570"
     CDS             complement(1379..1858)
                     /locus_tag="VIV63_17570"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6126468.1"
                     /translation="MIKNIAVRLARLADAAEIAAMSRDYIERGLPWTWTEARVANAIL
                     DADTNVVAARESDALLGFGIMYYTSDDAHLLLFAVRRAHQRRGVGSAILRWLEDVARA
                     AGSRRIRVECLRENSAARNFYCEQGYHELVITRKFYRGLKDGVHLEKWLREEETSGS"
     gene            1919..2440
                     /locus_tag="VIV63_17575"
     CDS             1919..2440
                     /locus_tag="VIV63_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126469.1"
                     /translation="MYRESLRIMLLACLAVSPLAHADPIHAIGDGAYWHHASHWVFAE
                     KAGAYVRVGFPQDVAGSEDAEAHYAYVENGVRHVAKIDVYRASSEAAGQLEAVPGGQL
                     ATEGAFLVSEANALYGIRRVYRTEGSETPVLTGLYLIAAGEWRITIRISGSTTEAMDA
                     FVHGQRWESLTAH"
     gene            complement(2434..2946)
                     /locus_tag="VIV63_17580"
     CDS             complement(2434..2946)
                     /locus_tag="VIV63_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126470.1"
                     /translation="MEQRTTLLLGFIAVGAGALIGWSLARSPEMASTAPVAATVSEVP
                     PPAQASATPPAIESAPTASSTDSVAKLIADTASGDAAKRASAITALASAPRADAIPVL
                     GRLLTDGEPQVDRQLALRSLHELALKQGDGDGKIRDAVRHAIYHGDDQTNVDDVQKVL
                     DAIERSQQPQ"
     gene            complement(2956..3906)
                     /locus_tag="VIV63_17585"
     CDS             complement(2956..3906)
                     /locus_tag="VIV63_17585"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY6126471.1"
                     /translation="MSGFMRIMSLLLLSFVSSYAHAHEMTMAEMELRETRPGEFLWQW
                     GASGSRPIGDELTPVWPDGCQSVENMLRCGEAGLSGVLSIDGVGDRYSAALVKVFWRD
                     GQSRVYTLTERQPTVHLYGSADDKRGMGEIARAYLVLGIEHILSGIDHLLFVAALLFL
                     VGFQRKLIWTITAFTLAHSLTLASAALGWLTLRSAPVEATIALSIVLVASEALQPRET
                     LARRLPAMVAFLFGLVHGLGFAGALSEIGLPQNHLATALLTFNIGVELGQLMAVAVVW
                     AVWRVVRNWRLADLSRVPLLYGIGALAAFWSLQRVTAIFS"
     gene            complement(3903..4763)
                     /locus_tag="VIV63_17590"
     CDS             complement(3903..4763)
                     /locus_tag="VIV63_17590"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6126472.1"
                     /translation="MSTTLAESGAGTSPATHRKFEAPAWVREPLLHFVLLGAVLFGVD
                     HAIAGRNEDPRTIVIDAEVDAHAIQIFKEARDHDPNADELYALRKVWLDNEVLYREGM
                     ALGLDRGDKAIRERVIFKALSMVDAGVKLPAVDENVLRTWFESNRQKYDKPARYDFQE
                     AVVTGDRSEAAARAFSQALNAGAPGEVEADLRVFTARPHDNIVQSYGADFATALAAAP
                     VGEWQVLPGTSGIRVVRLTSTTAPVPAKFEDLGGAVVQDWTDSVMAEQRSAAVRALAT
                     KYTVKVSEPK"
     gene            complement(4841..>5828)
                     /locus_tag="VIV63_17595"
     CDS             complement(4841..>5828)
                     /locus_tag="VIV63_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126473.1"
                     /translation="FYPGEYQRNYTLVRSGASKAKPQEIVDGLRTGNNFASSGQIIDR
                     LAFVACLGKSEALVEQIGTNAALANQAINAAGCASMGEKLVVPSGSDVVIGVTVRDPA
                     GTNYSPYNFPNPSLLQVGINQPINAPVLDHIDVIGGLVTGFKTPGTPGYSGEWPRNTN
                     WLKADGTTADLSVVPDAAKNVSTAILKAFNGSGASAWKAVTSPNDGSAFLTMTFRVSG
                     VTASQYIRLRGTNLPVAVPYETDASGNPLADVSTNASNTTRLRIPCSTPHSAGNQFDG
                     CPDHLATATGATNPIVGQKAVSYDVAAWADLWFYSNPVFVEVQGSSLVAGVK"
BASE COUNT          971 a         1903 c         1863 g         1091 t
ORIGIN      
        1 tgctgtgccg cgaggaattg ttccccgtgg cgagtccgca gctttttcgc ggcggatgcg
       61 ttcccgacac gctggcggaa ttggccgcct acccgttact gcacgacacc gacgccggca
      121 acgaccagcc ctggctcggc tggcggggct ggttcgaacg cgcggggctg tccaccgcga
      181 atgtgggccg cggcccgcag ttcagcgatt ccatcgtgct gatcggcgcc gccgtggccg
      241 gactcggcat cgccatcggc cgcgcgccgc atgttgcgcc gatgttgtcg cgcggccagc
      301 tcgtgcgtgt cacccaggaa acctggatgg cgccctggaa ctactacctc atggcgccgg
      361 ccgcgcactt tcgccgcccg ctggtgcgca ccttcgtgga ctgggcgctc gaagaggcac
      421 gttcgggtca ataagttttc gttgtgccag agcaagctct acttgcttac aggcggaccc
      481 gccggttctc tacaatcgcg cgatgtctaa acgcggctgg atcctgtttc tctcgctggg
      541 cctgctctgg ggcatgcctt acctgctgat ccgcatcgcc gtcggcgagt tcgatccgtt
      601 gatggtcgcg gttgctcgca ccttcctggg ttcgctgctg ctgctgccca ttgcgttgca
      661 tcgcaaagcg ttgctgcccg cgttccgcaa gtggaagtgg ttgctggcat ttacgctcat
      721 cgagatcagt attccgtggg tattgctcgg ccacgccgaa acacgcatca acagctcaac
      781 ggccgggttg ctgatcgccg tcgttccgct gttcaccgcc atcatcgtca cgagactagg
      841 ccacgaaaaa ctcgagccgc ggcgcatgtt cggtctcggc ctggggttcg cgggtgtcgc
      901 gctgctggtg ggcctcgaca tccatttcga cgacctgccc gccgtggccg cgaccattgc
      961 ggtggcgctc ttttacgcca tcggacccat catcatcgac cgcaagctca gcgacgtgcc
     1021 cgccattggc gtcatcaccg catcgctgat gctcgggacg ctcatctacg cgcccttcgt
     1081 gcccttcctg tggcccgaga ctttcagcgc gccggccgtc gcgtccgtca tcggtctcgg
     1141 tgtgttgtgc acggccgcgg cgttcatggt gttcttcgcg ctgatcgccg aagtcggccc
     1201 ggcgcgcgcc acggtcatca cctatgtgaa tccggcggtg gccatcctgt taggcgcgct
     1261 ggtgctgagc gaaccgctga ccctcggcat ggccatcggc tttccgctgg tgatcgcggg
     1321 gtcgttcctg ggtacgcagc gatcgcgaac gccggtggcg gccacgccgg tcgccgaatc
     1381 aagagcctga cgtttcttct tcgcgcagcc acttctcgag atgcacgcca tccttgaggc
     1441 cgcgatagaa cttcctggtg atcacgagct cgtgataacc ctgctcgcaa tagaaattcc
     1501 gcgcggctga attttctcgc aggcactcga cgcggatgcg cctggagccg gcggcgcggg
     1561 caacgtcttc gagccagcgc aggatcgcgc tgccgacacc gcggcgctga tgcgcgcgcc
     1621 gtacggcaaa caacagcagg tgcgcgtcgt cgctggtgta gtacatgatg ccgaagccca
     1681 gcagcgcgtc gctttcgcgg gccgccacca cgttggtgtc ggcatcgagg atggcattcg
     1741 ccacgcgtgc ttccgtccag gtccacggca gtccccgctc gatatagtcg cgcgacatcg
     1801 cggcgatctc cgcggcatcg gcgagcctcg cgaggcgcac ggcgatgttc ttgatcatgt
     1861 ccgcacagat acacgcgcga ccacgaatct gcaatacgcg gcgtttacgc tttcgcgcat
     1921 gtatcgggaa tccctgcgaa tcatgttgct ggcgtgcctg gcggtgagcc cactggcaca
     1981 cgccgatccc attcacgcca tcggcgatgg cgcgtactgg catcacgcca gccattgggt
     2041 gttcgcggag aaagccggtg cctacgtgcg cgtcgggttt ccgcaggacg tcgccggatc
     2101 ggaagacgcc gaagcgcatt acgcatatgt cgagaacggc gtgcgccatg tggcgaagat
     2161 cgatgtgtac cgggcttcct ccgaggcggc cgggcaactc gaagcggtgc ccggcggaca
     2221 attggcgacg gaaggcgcgt tcctcgtgag cgaagcgaac gcgctgtatg gcattcgccg
     2281 ggtctacaga accgaaggct ccgaaactcc ggtgctcacg ggtctgtacc tgatcgcggc
     2341 gggtgagtgg cgaatcacga tccgcatctc gggatcaacg accgaggcga tggacgcgtt
     2401 cgtgcacggc cagcgctggg aaagcctcac ggctcactga ggctgctgcg atctctcgat
     2461 ggcgtcgagc accttctgca cgtcatcgac gttcgtctga tcgtcgccgt gatagatcgc
     2521 gtggcgaacc gcgtcgcgaa tcttgccgtc gccatcaccc tgcttcagtg cgagctcgtg
     2581 caacgagcgc aatgccagct gcctgtctac ctgcggctca ccatccgtga gcaagcggcc
     2641 gagcacggga atcgcgtcgg cgcgtggcgc actcgcgagc gcggtgatcg cggaagcgcg
     2701 tttagcggcg tcgccgctcg cggtatccgc aatcagtttc gccacggaat ccgttgagct
     2761 cgccgtgggc gcggactcga tggcaggtgg cgttgcgctg gcctgtgcgg gcggaggcac
     2821 ttcgctgacg gtcgcggcaa ccggtgcggt gctcgccatt tcggggctgc gcgcgagact
     2881 ccagcctatg agtgcgccgg caccgacggc gatgaatcca agaagaaggg tcgtacgttg
     2941 ttccatggtc gcggcctaac taaagatggc ggttacgcgc tgcaatgacc agaacgccgc
     3001 cagcgccccg atgccgtaca gcaacggaac acggctcaga tccgccagcc gccagttgcg
     3061 cacgactcgc cacacggccc agaccacggc gaccgccatc agttgcccga gctccacgcc
     3121 gatgttgaac gtgagcagcg ccgtcgccag gtgattctgc ggcagaccga tttcgctgag
     3181 cgcacccgcg aagccgagcc catgcacgag gccgaacagg aaggcgacca tggccggcag
     3241 ccggcgcgcc agggtttcgc gcggctgcag cgcttcgctg gcaaccagca caatggacaa
     3301 cgcgatagtc gcttccaccg gcgccgagcg cagcgtgagc cacccgagcg cggcgctggc
     3361 cagcgtgagg ctgtgtgcca gcgtgaacgc ggtgatggtc cagatcagct tgcgctgaaa
     3421 acccacgagg aagagcagag ccgcgacgaa cagcaggtgg tcgatgccgc tcaggatgtg
     3481 ttcgatgccc aacacaagat acgcgcgggc gatctcgccc atgccgcgtt tgtcgtccgc
     3541 cgatccgtac agatgcaccg tgggctgacg ttcggtcagc gtgtagacgc ggctctgacc
     3601 gtcacgccag aacaccttca ccagcgcggc ggagtagcga tcgccgacgc cgtcgatcga
     3661 caacaccccg ctcagccctg cctcgccgca gcgaagcatg ttctccacgc tctggcagcc
     3721 gtcgggccac acgggcgtga gctcatcgcc aatcgggcga cttccactcg cgccccattg
     3781 ccacaggaac tcgcccggcc gcgtttcccg cagctccatt tcggccatgg tcatctcatg
     3841 cgcgtgggca tagctgctca cgaacgagag cagcaacagc gacatgatcc gcatgaaacc
     3901 gctcattttg gttcgctgac cttcacggtg tacttcgtcg ccagcgcgcg cacggcggcg
     3961 ctgcgctgct cggccattac ggaatccgtc cagtcctgca cgaccgcgcc gcccaggtct
     4021 tcgaacttcg cggggaccgg cgccgtcgtc gacgtcaaac ggaccacgcg tatgccggag
     4081 gtgccaggta gcacctgcca ttcacccacc ggcgccgccg ccagcgcggt ggcgaagtcg
     4141 gcgccgtaac tctgaacgat gttgtcgtgc gggcgtgccg tgaacacacg cagatccgct
     4201 tccacttcgc cgggtgcgcc ggcattcaat gcctgtgaaa acgcgcgcgc cgccgcttcc
     4261 gagcggtcgc cggtgacgac ggcctcctgg aaatcgtatc gcgcgggctt gtcgtatttc
     4321 tggcgattgc tctcgaacca ggtccgaagg acgttctcgt cgaccgcggg tagcttgacg
     4381 cccgcgtcca ccatgctcag cgccttgaag atcacgcgtt cgcgaatcgc cttgtcgccg
     4441 cggtcgagac cgagcgccat tccttcgcga tacaacacct cgttgtcgag ccacaccttg
     4501 cgcagcgcgt agagctcgtc tgcgttcgga tcgtggtcgc gcgcttcctt gaagatctgg
     4561 atggcatggg catccacttc cgcgtcgatg acgatggtgc gtggatcttc gtttcggccg
     4621 gctatcgcgt gatcgacacc gaacaacacg gcgcccagaa gcacgaaatg cagcaggggt
     4681 tcgcgcaccc aggcaggtgc ctcgaatttt cgatgtgtgg ccggtgacgt gccggcgcca
     4741 gattcagcaa gggtggtgga catatgtctt acgcctcaaa agagagaaag cgccgcgcgt
     4801 ttccgcgcgg cgctctgggg tcgctcgagt tgttagtggc ttacttgacg ccagccacca
     4861 gcgaagagcc ttgcacttcg acgaagaccg gattgctgta gaaccagagg tccgcccagg
     4921 ccgcgacgtc gtatgaaacc gccttctgac cgacgattgg attggtagcg ccggtggcgg
     4981 tcgccaggtg atccgggcaa ccgtcgaact gattgcccgc gctgtgtggt gtgctgcacg
     5041 ggatgcgcaa gcgagtcgtg ttgctggcgt tggtagaaac gtctgccagc ggattgccgc
     5101 tcgcgtcggt ctcgtacggc accgccaccg gcaagttggt gccgcgcaga cggatgtact
     5161 gcgaggcggt cacgccggag acgcggaacg tcatcgtcag gaaggccgaa ccatcgttcg
     5221 gagacgttac ggccttccag gccgaggcgc cgctgccgtt gaaggccttg agaatggccg
     5281 tcgagacgtt cttggccgcg tccggtacta ccgacagatc ggcagtggtg ccatccgctt
     5341 tcaaccaatt ggtattgcgc ggccactcac cggagtagcc cggcgtcccc ggcgtcttga
     5401 agccggtcac taggccaccg atcacgtcga tatggtcgag caccggcgcg ttgatcggct
     5461 ggttgatgcc cacctgcaag agcgacgggt tggggaagtt gtacggcgag tagttcgttc
     5521 ctgccggatc gcgcacagtg acgccaatca cgacgtcgga cccggacggc acgaccagct
     5581 tttcgcccat cgaggcgcaa ccggcagcgt tgattgcctg gttagcgagc gcggcgttgg
     5641 tcccgatttg ttcgaccaga gcctccgact tgccgaggca agcgacgaac gccaagcgat
     5701 cgatgatctg cccgctgctc gcaaagttgt tgccggtgcg cagaccatcg acaatctcct
     5761 gcggtttggc cttgctggcc ccgctccgca ccaacgtgta gttgcgctga tactcgccgg
     5821 ggtagaag
//
LOCUS       DATDEU010000738         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_2184224, whole genome shotgun sequence.
ACCESSION   DATDEU010000738 DATDEU010000000
VERSION     DATDEU010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184224"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VIV63_17600"
     CDS             <1..480
                     /locus_tag="VIV63_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY6126474.1"
                     /translation="KVWDTKYARIGVGICWDQWFPECARAMALMGAELLFYPTAIGSE
                     PPPALPVNSRDHWQRCQQGPAAATLMPVIVSNRIGTERALQNPDGLYIRFYGSSFIAD
                     STGAKIAEAGEEGEAVLTARFDLAANEELRNNWFVFRDRRPELYGALISFDGAIHAK"
     gene            470..694
                     /locus_tag="VIV63_17605"
     CDS             470..694
                     /locus_tag="VIV63_17605"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY6126475.1"
                     /translation="MPSDAEELAEIDALFAQADLDHDGQINFTEFKTLVRELDGDMTE
                     AELRIGFTETDVNQNSRINIDEFRAWWLSG"
     gene            complement(1059..1389)
                     /gene="ssrA"
                     /locus_tag="VIV63_17610"
     tmRNA           complement(1059..1389)
                     /gene="ssrA"
                     /locus_tag="VIV63_17610"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1480..>2410)
                     /locus_tag="VIV63_17615"
     CDS             complement(1480..>2410)
                     /locus_tag="VIV63_17615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY6126476.1"
                     /translation="TRVLARVEDEAHPPIYYLVDYAAKKADILNEAYPLLVGIKLGAV
                     RDFNYEARDKYALMGYLTVPPGAHEKNLPLVVMPHGGPEARDEPGFDWLAQFLASRGY
                     AVLQPQFRGSTGFGQAHADAGRRQWGLRMQDDVTDGVRALIEQGIADPKRVCIVGWSY
                     GGYSALAGATFTPELYACAASIGGISDLPDMVGFDFKNGGRESNSFAYVREHIGTKTD
                     PQVIAKSPARFASAVRAPILLIHGVDDTVVPISQSQRMARALKAAGKPHELIELPGED
                     HWMMTSSASRIRTLAELEGFLGKHIGAPPAATN"
BASE COUNT          450 a          745 c          764 g          451 t
ORIGIN      
        1 aaggtctggg acacgaagta cgcacgcatc ggtgtcggca tctgctggga ccagtggttt
       61 ccggaatgcg cgcgcgccat ggcgctcatg ggcgcagagc tgctgttcta tcccaccgcg
      121 atcggcagcg agccgccgcc cgcattgccc gtgaattcgc gcgatcactg gcagcgctgc
      181 cagcaggggc ccgcggccgc gaccctgatg ccggtcatcg tctcgaatcg aatcggcacc
      241 gagcgcgcgc tgcagaatcc cgacggactc tacattcgct tctatggttc cagcttcatc
      301 gccgactcca ccggtgccaa gatcgccgag gcaggcgaag agggggaggc cgtgctcacg
      361 gcccggttcg acctcgcggc gaacgaagag ctgcgcaaca actggttcgt cttccgggac
      421 cggcggccgg agttgtatgg cgccctcatc agtttcgacg gagcaataca tgccaagtga
      481 tgccgaagaa ctggccgaga tcgacgcgct gttcgcgcag gccgacctcg atcatgacgg
      541 gcagatcaac ttcaccgagt tcaagacgct ggtacgtgag cttgatggcg acatgacgga
      601 agcggaactg cgcatcggat tcactgaaac cgacgtgaat cagaacagcc gcatcaacat
      661 cgatgaattc cgcgcgtggt ggctcagcgg ctgagcgacc gcgagagaga agcaggctcc
      721 ccggatttct ccggggaacc tgcacttttg gtggaggcgg cgggacagtt gttccaactc
      781 tttcgagatg gcctggacta tgccttcatc cttcatcact ttcgcgatga gtcggatgcg
      841 gggcgtgtta tccgaactac tcgttccgga tcctggggcc ggctctgccg catgagcggt
      901 tcagccagtc catgagtctc tacagggcag gacaagttgg cctcgtccca cccctcggcg
      961 ttgccaggtc gctgttgcca gcgacttgag ggttcaccga atgagccccg ttatcacttc
     1021 gcccttacga gcgaaggcaa ccattgcgtt ccttcgcatc aattgaaccc gcgtccgcaa
     1081 gcgttagacg taagactcta cgtacgtagc tgcttctttg ttttctcgcc tcacgctacc
     1141 cgaagagcag ggaaaacgct cagccagcga ctgtgactct taacgattcc gccagtagca
     1201 cacggaaacg cgatcctatg agcgctaccc caggtccagc ggcataggca ccaactggtc
     1261 tgagggcaac cgcgattagg cggctagtgc gtagttgtcg tcgttggcaa ctatgaagtg
     1321 cacagtgttt aacgagggcc atgcgcctcg gtacgcctct tacggttcac tacccacgtc
     1381 gaaaccggtc gcccccgtaa aggtgatgcg agcatacatg ggggccgggc gcgcgtctgc
     1441 aagacaccgg gtccagagcg cggaagtgcg gattttccgt cagttcgtcg ctgccggcgg
     1501 cgcgccgatg tgtttcccga gaaagccttc gagctcggcc agcgtgcgga tgcgcgaagc
     1561 gctgctggtc atcatccagt ggtcttcgcc gggaagctcg atcaactcgt gcggcttgcc
     1621 cgcggccttg agggcgcgcg ccatgcgctg cgactggctg atcggcacca cggtgtcgtc
     1681 cacgccgtgg atgagcagga tgggagcacg caccgcgctc gcgaagcggg caggcgactt
     1741 cgcgatcacc tgcggatcgg ttttcgtgcc aatgtgttcg cgaacgtacg caaaggagtt
     1801 cgattcgcga ccgccgttct tgaaatcgaa accgaccata tcgggcagat cggagatgcc
     1861 accgatgctc gccgcgcacg cgtacaactc cggcgtgaag gtggcgccgg cgagcgcgga
     1921 atagccgccg tagctccagc cgacgatgca aacgcgcttc ggatcggcaa tgccctgttc
     1981 gatgagcgcg cgaaccccat cggtgacgtc atcctgcatt cgcaggcccc attggcgccg
     2041 gcccgcgtcg gcatgcgcct ggccgaagcc ggtggagcca cggaactgcg gctgcaggac
     2101 ggcgtagccg cgcgacgcaa ggaattgggc taaccagtcg aagccgggtt cgtcgcgcgc
     2161 ttccgggcca ccgtgcggca tgacgaccag cgggagattc ttctcatgtg cgccgggcgg
     2221 caccgtgagg tagcccatga gcgcgtactt gtcgcgcgct tcgtagttga aatcccgcac
     2281 tgcgcccagc ttgatgccga ccagcagtgg ataggcctcg ttgaggatat cggccttctt
     2341 cgcggcgtag tcgaccagat agtagatggg cgggtgcgct tcgtcttcca cgcgggcaag
     2401 aacgcgcgtg
//
LOCUS       DATDEU010000739         6066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1892288, whole genome shotgun sequence.
ACCESSION   DATDEU010000739 DATDEU010000000
VERSION     DATDEU010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892288"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VIV63_17620"
     CDS             <1..329
                     /locus_tag="VIV63_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126477.1"
                     /translation="GAGLVALPRFFADRYSPPVKGNLDEQLRTSITSLNTTGFWPASL
                     AQTSNPYKGPGGADKVAGDFAGTHVGDETDTSPFTPTTKIESISTAEYLRNMNILVHW
                     LAKAPH"
     gene            384..1709
                     /locus_tag="VIV63_17625"
     CDS             384..1709
                     /locus_tag="VIV63_17625"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY6126478.1"
                     /translation="MESNSAPRRHVSVLYVVMVAVAMVVGAGIFRSPAEIAANAGSST
                     WFFAAWIAGGLISLAGALCFAELATMLPSAGGDYFFLKTAYGRNVAFLFAWARFAVIN
                     TGSLALLGFVLGDYANTVWSLGPHGSSWYALITIVAMTLFNLKGLYTGQSADYAMTGL
                     EVGGLLIVFAAGVALAIAGAPPLVPDAQAQPGFPGTFGLALLFVLFAFSGWSEVSTLS
                     AESRDERRGMVRALVASIVTITVLYLLANWALWRGLGIAGLAASRTPASDVIARAFGD
                     WAGVLTAIAIAMATLTSINATIVVGARTTYAAAADWPALNRLGHWNTRRGIPVAGIVS
                     QGIVGILLVGLGTYTRDGFITMVDYSSPVFWLFLALSGFAVILLRRRHPELPRPFRVP
                     LYPWLPLVFVSSCLFVLWSSLAYVRLGALLGVAVLAVGGLVLLGMRRSA"
     gene            1719..4037
                     /locus_tag="VIV63_17630"
     CDS             1719..4037
                     /locus_tag="VIV63_17630"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEY6126479.1"
                     /translation="MRKPWLPILALAGALLGAAYAQRAVAQDSADGWITHAESGVRTP
                     IVLHFQRDLNLKAVPAKLPVSVTADNRFVLYVNGQRVASGPSAGTVQSWREARLDLAP
                     HLKRGANVITAVVWNFGEIAPAFQQSVATGFRLTGAPIPTSSPGWRVRIDTGHSAVNA
                     KEQIGWQYYVASTPEVIDARRPPSDWQDAVPAPAATARQRIVDPFPPQLYKAAEPGKV
                     VRSSLNGGEAFPAVPVTIPAKTTAKLLIQRDVMISAYPELAVQGGRDATIKVEYGEAL
                     YDADGRKGDRNLIGDRRLRGFHDHFIADGSTRVFAPLWWRTFRYLEIEVSTQAEPLTL
                     TALRLHETGYPFEQVASFVSSDAELNRIWDIGWRTMRVDAHETFMDSSYWEQLQYTGD
                     TRLEMLITYAVSGDARLARQAIDAFAESDVDGGLVQGAYPSRLPNVIATFAFAWVGML
                     ADWSLEQPDRAIIERHLPRMRRVLTWFAPMLNPQGLLGKNPQWNFIDWSGQKWDDRDT
                     FPSWGSQNGSCLMTAMWLGALRQGAALEESHGDKMRAAGFADKADRARNAIREHCWVA
                     EKGLFADDGDRKVFSQHMNVFAVLYDIATREEAPAILDRVTQRGRGIEAPAGMYASTY
                     YFAWYLVRAFEHAGMSERYFDLLRSWRDLLAFNYTTWPETREQPRSDTHAWSAHPTAD
                     LLGIVAGIRSAAPGYSRLRIEPVLGSLTSLDATSMTPHGPVRVSYRVTADELTAVIDR
                     PSALPGTFVWRGKSHSLKGIHTKLTLPIKENP"
     gene            4034..4954
                     /locus_tag="VIV63_17635"
     CDS             4034..4954
                     /locus_tag="VIV63_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6126480.1"
                     /translation="MTTRRQFLGTTLLASATIVPLTQFAAAATQSKAMLMRTIPSSGE
                     KIPAIGMGTSGSFQIPADGAEYVALKEVLKRFFEGGATLIDTAPTYGNAEDNLGPLLA
                     EGGYRKKVFIATKLSGVTGRDAGLAQFNNTLQRLKTDKVELLQVHNLRDWKTQLEVAR
                     ELKKQGKVKYVGVTHYTDSAHDEIADIVQESKPDFLQINHSVTNRGIEKRVLPLAKEL
                     GVAVLTNRNFNDGALFGQVQGKPLPGWAAEAGVTSWAQMFLKYSLSHEAVTAVIPATG
                     KPDRQSDNLKAGVGPLLTAAQQKELIALFA"
     gene            5080..>6066
                     /locus_tag="VIV63_17640"
     CDS             5080..>6066
                     /locus_tag="VIV63_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126481.1"
                     /translation="MADFSSRFIAVAVVATCVAAPAAADSPLGLNVLGTYSTGLGEAS
                     SEIAAYDKQSRRVFVTNAVSRSVDIVDVSTPAAPVRIERIDVSALGTPNSVSIKNGLV
                     AVAIEAPVKTDAGLVAFYTADGTPIAQVRVGSQPDMLTFTPDGKNVVVANEGEPSGYG
                     GATDVDPEGVVSIIRVPAVNGDWKNFGPGNLRQVVFTQYNGQEAALRAQGIRIYGPGA
                     SAAQDFEPEYVAVSDDSRTAYVTLQENNAIAVVDIARAQVRKLLPLGYKNYSEQPQTI
                     ASYEWRDLPVVGKTAAGQKINLGGFSGLAFEGTTLDGRLKFITHTDRGPNGEP"
BASE COUNT         1064 a         1959 c         1934 g         1109 t
ORIGIN      
        1 cgggcgcggg tttggtggcg ctgccacgtt tcttcgcgga tcgatattcg ccgccggtga
       61 aggggaatct cgacgagcaa ctgcgcacgt cgatcacctc gctcaacacc acgggcttct
      121 ggcccgcgtc gctggcgcag accagcaatc cctacaaagg tccgggcggg gcggacaagg
      181 tcgccggcga tttcgcgggc acacacgtgg gtgatgaaac ggacacgtca ccgttcacgc
      241 cgaccacgaa gatcgaatcg atttccacgg ccgaatacct gcgcaacatg aacatcctgg
      301 tgcactggct cgcgaaggcg ccccactaac atgcagtgac ggcgcgagag cgaagggcct
      361 gttagacggg tactctccgt gtcatggaat cgaactccgc gccccgccgc catgtcagcg
      421 tgctctatgt cgtcatggtg gcggtggcca tggtggtcgg cgcgggaatc ttccgctcgc
      481 ccgccgagat tgccgccaac gccggctcgt ccacgtggtt cttcgccgcc tggattgccg
      541 gcggtctgat ctcgctggcc ggcgcgctgt gtttcgcgga gctcgcgacg atgttgccga
      601 gcgccggcgg cgactacttc tttctcaaga ccgcctacgg ccgcaacgtc gcgttcctgt
      661 ttgcgtgggc gcgtttcgcc gtcatcaaca ccgggtcgct cgcgttgtta ggcttcgtgc
      721 tgggcgacta tgccaacacc gtctggtcgc tgggcccgca cggctcgtcc tggtacgcgc
      781 tgatcaccat cgtcgcgatg acgctgttca atctgaaagg tctttatacg ggccagtccg
      841 cggactacgc gatgaccggt ctcgaggtcg gtggtttgct catcgtgttc gccgccggcg
      901 tggcgctggc catcgccggc gcgccaccgc tggtgccaga cgcgcaggcg cagcccgggt
      961 ttcctggcac cttcggtctc gcgctgttgt tcgtcctgtt cgcgttcagt ggctggagtg
     1021 aagtatcgac gctgtccgcc gaaagtcgtg acgagcggcg gggcatggtg cgtgcgctgg
     1081 tcgcgtcgat cgtcaccatc acggttctct atctactggc gaactgggcg ttgtggcgcg
     1141 ggttggggat cgcgggcctg gcggccagcc gcacgcccgc gtcggatgtc atcgcgcgcg
     1201 ccttcggcga ctgggccggg gtgttgaccg cgatcgccat tgccatggcg acgcttactt
     1261 ccatcaacgc gaccatcgtc gtaggtgcgc gaacgaccta cgcggccgcc gccgattggc
     1321 ctgcgctcaa ccgactcggt cactggaaca cgcggcgcgg cattccggtc gcgggcatcg
     1381 tttcgcaggg catcgtgggc atactgctcg tggggctcgg cacgtatacg cgcgacggat
     1441 tcatcacgat ggtcgactac tcgtcgccgg tgttctggtt gttcctggcg ctgagcggat
     1501 ttgccgtcat cctgctgcgg cgccgccacc cggaacttcc gcgtccgttc cgggtgccgc
     1561 tgtatccctg gctgccactc gtgttcgtgt cgtcgtgtct gttcgtgttg tggtcgagtc
     1621 tggcgtacgt gcggctgggt gcgcttctcg gcgtcgcggt actggccgtg ggtggtctcg
     1681 tgctattggg gatgcgccga tcagcctaga ctcgcggcat gcgaaaacca tggctgccga
     1741 ttctcgcgct ggccggcgct ctgctcggcg ccgcgtacgc gcaacgcgcc gtcgcccagg
     1801 actccgccga tggctggatc acgcatgcgg agtcgggggt acgcacaccc atcgtcctgc
     1861 attttcagcg cgacttgaat ctcaaggcgg taccggcaaa gttgccggtg agcgtcaccg
     1921 ctgacaatcg cttcgttctt tatgtgaatg gccagcgagt agccagcggg ccgtcggcgg
     1981 gtacggtgca gagctggcgc gaggcgcggc tcgatctcgc gccacacctg aaacgaggcg
     2041 cgaacgtcat cacggctgtg gtctggaact tcggcgaaat cgcgcccgcg tttcagcaga
     2101 gtgtggcgac aggattccgg ctgaccggcg cgcccatccc gacgagttcg cctggctggc
     2161 gcgtgcgcat cgacaccggc cattcggcgg tcaacgcgaa ggaacagatt ggctggcagt
     2221 actacgtcgc cagcacgccc gaagtcatcg acgcacgcag gccgccgtcg gattggcagg
     2281 acgccgtacc ggcacctgct gccaccgctc gccagcgcat cgtcgatccg ttcccgcccc
     2341 agctatacaa ggcggcggag ccgggcaagg tcgttcgcag ttctctcaac ggcggcgagg
     2401 cttttcccgc ggtgcccgtg accattcccg ccaagacgac ggccaaacta ctcatccagc
     2461 gcgacgtcat gatctccgcc tatccggaac tcgcggtgca gggcggtcgc gacgcgacga
     2521 tcaaggtcga gtatggcgag gcgctgtatg acgccgacgg acgcaagggc gaccgcaatc
     2581 tcatcggcga ccgccgcctg cgcggtttcc acgaccactt catcgcggac ggatcgacgc
     2641 gcgtgttcgc tcccttgtgg tggcgcacgt tccgatacct cgagatcgaa gtaagcaccc
     2701 aggccgagcc gctcacgctg actgccctgc gtctgcacga gacaggctac ccattcgaac
     2761 aggtggcgag tttcgtgagc agcgacgcgg aactcaatcg catctgggac atcggctggc
     2821 gcacgatgcg cgtcgacgcg catgaaacct tcatggatag ttcgtactgg gaacagcttc
     2881 agtacacggg cgacacgcgc ctcgagatgc tgatcaccta cgccgtgtca ggcgatgcac
     2941 ggctcgcgcg ccaggccatc gatgcattcg cggaatccga cgtcgatggc ggactcgtgc
     3001 agggcgcgta tccgtcccgc ttgcccaatg tcatcgccac gttcgcattc gcctgggtgg
     3061 gcatgctcgc ggactggtcc ctggaacagc cggatcgcgc catcatcgag cggcacctgc
     3121 cgcgcatgcg ccgcgtgctg acatggttcg cgccgatgct caacccacaa ggtttgctgg
     3181 gcaagaaccc gcagtggaat ttcatcgact ggtcggggca gaaatgggat gaccgcgaca
     3241 ctttcccctc ctggggaagc cagaatggaa gctgcctgat gacggccatg tggctgggcg
     3301 cattgcggca aggcgcggca ctcgaagaga gccacggcga caagatgcgt gcggccggct
     3361 tcgccgacaa agccgatcgt gcgcgcaacg ccattcgcga acattgctgg gtcgctgaaa
     3421 aggggttgtt tgccgacgat ggcgaccgca aggtcttcag tcagcacatg aatgtgttcg
     3481 ccgtgctcta cgacatcgcc acgcgtgagg aagctccggc gatcctcgat cgcgtcacgc
     3541 agcgcggccg cggcatagag gctcccgccg gcatgtacgc atccacctac tatttcgcgt
     3601 ggtacctcgt acgcgccttc gagcacgccg gcatgtccga acgttacttc gatctgctgc
     3661 ggagctggcg ggatctgctg gccttcaact acaccacctg gccggaaacc cgcgagcaac
     3721 cgcgttcgga cacgcatgcc tggagtgcac atccgacggc cgacttatta ggcatcgtgg
     3781 cgggtatccg gtccgcggcg ccgggctact cgcggctgcg catcgaaccg gtgctggggt
     3841 cactgacctc gctcgacgcc acgtcgatga cgccccacgg tcccgtcagg gtgagttatc
     3901 gcgtgacggc cgacgagctg acggcggtca tcgaccggcc ctccgcatta ccaggaactt
     3961 tcgtctggcg cggcaagtcg cattcgctga agggaattca cacgaagctc acgctcccaa
     4021 tcaaggagaa cccatgacca ctcgccgcca gtttctgggc accacgttgc tcgcctccgc
     4081 cacgatcgtg ccattgacgc agttcgctgc agccgccacc caatcgaaag cgatgctgat
     4141 gcggaccatt ccgtcgtcag gcgagaagat tccggccatc ggcatgggca cgtccggaag
     4201 tttccagatt cccgcggacg gcgccgaata cgtggcgctc aaggaagttc tcaagcgatt
     4261 cttcgaaggc ggcgcgacgc tcatcgacac ggcaccgacg tacggcaatg ccgaagacaa
     4321 cctgggcccg ctgctcgccg agggcggata ccgcaagaag gtattcatcg ccacgaagct
     4381 gtccggtgtc accgggcgcg acgcggggct cgcgcagttc aacaacacgc tgcagcgcct
     4441 caagaccgac aaggtggagc tgctgcaggt acacaatctg cgcgactgga aaacgcagct
     4501 cgaagtcgcg cgcgaactca agaagcaggg caaggtgaaa tacgtcggcg tcactcatta
     4561 caccgactcc gctcacgacg agatcgccga catcgtgcag gagagcaagc ccgactttct
     4621 gcagatcaat cactcagtca ccaatcgcgg catcgaaaag cgcgtgttgc cgctcgccaa
     4681 agagttgggc gtcgcggtgc tcaccaatcg caatttcaat gatggcgcgc tgttcggtca
     4741 ggtccagggc aagccgctgc ccggctgggc tgccgaagcg ggcgtcacgt catgggcgca
     4801 gatgttcctg aagtattcgt tgagtcacga agccgtgacc gcggtcatcc ccgcgaccgg
     4861 caagcccgat cgccagtctg acaatctcaa ggcaggtgtt ggcccgctgc tgactgccgc
     4921 ccaacagaaa gagttgatcg cgctgttcgc atgacgcggg cgtgagcgtc tccgaagacg
     4981 ctttcgtcat ccagatgtaa tacggctctg gctccatttg gcgcagcaat aacagaccgg
     5041 cttcagcgcc ggctcattga acgcccggag gattccacca tggccgattt ttccagccgc
     5101 ttcatcgcgg ttgccgtcgt tgcgacttgc gtcgccgcac ccgcagcagc cgattcgccg
     5161 ctcgggctca acgtgctggg cacttactcg accggtctcg gcgaggcgag ctcggaaatc
     5221 gcggcctacg acaagcagtc gcgccgcgtg ttcgtgacca acgcggtgag tcgctccgtg
     5281 gatatcgtcg acgtcagcac gccggcggcg ccagtccgca tcgagcgcat cgacgtctcg
     5341 gctctcggca cgcctaacag cgtctcgatc aagaacggac tcgtggccgt tgccatcgag
     5401 gcgcccgtga aaaccgatgc ggggctcgtc gcgttctata ccgcggacgg cacaccgatc
     5461 gcacaggtgc gcgtgggctc gcagcccgac atgctgacgt tcacacccga tgggaaaaac
     5521 gtcgtggtcg ccaacgaagg cgaacccagc ggttacggtg gcgccacgga cgtcgatccc
     5581 gaaggtgtgg tgagcatcat tcgcgtgcca gcggtgaatg gagactggaa aaactttggc
     5641 ccgggcaacc tgcgccaggt ggtattcacg cagtacaacg gccaggaagc cgcgctgcgc
     5701 gcgcagggca ttcgcatcta tggtccgggc gcgagcgccg cgcaggattt cgagcctgag
     5761 tacgtcgccg tatcggacga ttcgcgcacg gcgtacgtga ccctgcagga gaacaacgcg
     5821 attgcggtgg tggacatcgc gagggcgcag gtccgcaagc tactgccgct tggctacaag
     5881 aattacagcg aacagccgca aacgattgcc tcttatgaat ggcgcgacct gcccgtggtc
     5941 ggaaagaccg cggcgggcca gaagatcaac ctcggtggct tctccggcct cgccttcgaa
     6001 ggaactacgc tcgacggccg cctgaagttc atcacgcata ccgaccgtgg tccgaacggt
     6061 gaaccg
//
LOCUS       DATDEU010000740         4859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_727058, whole genome shotgun sequence.
ACCESSION   DATDEU010000740 DATDEU010000000
VERSION     DATDEU010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727058"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /gene="aceE"
                     /locus_tag="VIV63_17645"
     CDS             complement(<1..1595)
                     /gene="aceE"
                     /locus_tag="VIV63_17645"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY6126482.1"
                     /translation="MTEIMKLEDLDPSETREWIESIDSVLKTQGAARAHYLLEQLIDY
                     TRRSGAYLPFRPNTAYVNTISPGHEPSYPGNREFEKRIEACIRWNALAMVLNANKVSS
                     EYGGHLSTYASSATLYEVGFNHFWRAASADHPGDMVFVQGHASPGIYARAFLEGRLSE
                     DQLKHFRQEASGDGLSSYPHPWLMPNFWQFPTVSMGLGPMMAIYQARFQRYMENREMV
                     PVTDRKVWAFLGDGEMDEPESMGALTLPVREKLDNLVFVVNCNLQRLDGPVRGNGKII
                     QELEAAFMGAGWNVIKVVWGSRWDPLLARDTQGLLQRLMEECVDGEYQNFKAKGGAYT
                     RENVFGKYPELKEMVANMSDDDIWRLNRGGHDARKVFAAYSAAMNHKGQPTIILAKTV
                     KGFGLGKAAEGQMVAHQQKKLDEEALRAVRDRFNIPLTDEQVGKLALMKPADDSPEMK
                     YLHERRKALGGYLPARKIVAKPLVIPELSAFSAMLEGTGDREISTTMAFVRILTMLLK
                     DKNIGKNVVPIVPDEARTFGMEGL"
     gene            1785..2828
                     /gene="pyrC"
                     /locus_tag="VIV63_17650"
     CDS             1785..2828
                     /gene="pyrC"
                     /locus_tag="VIV63_17650"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY6126483.1"
                     /translation="MEIEIRRPDDWHLHLRDGAAMAAVLPFTAERFARAIVMPNLKPP
                     VTTVASAEEYRQRILAALPAGSAFTPLMTLYLTDQTDPAEIDKALKSQCVYACKLYPA
                     GATTNSDSGVTDIRRIDDVLARMSDAGMPLLVHGEMTARQIDVFDRETRFIDEVLIPT
                     LARFPKLRVVFEHITTARAAEFVQGARDGVAATITPQHLLHNRNAIFEGGIRPHYYCL
                     PILKTEPDREALLAAATSGNPRFFLGTDSAPHAQHTKENACGCAGMFSAHAGIELYAE
                     AFESVGKLDKLEGFASDFGADFYGLPKNSTRIRLAKREWRPPASYPLGKTDKDGRLVP
                     MRANEAIPWQLVS"
     gene            2828..3547
                     /gene="rnt"
                     /locus_tag="VIV63_17655"
     CDS             2828..3547
                     /gene="rnt"
                     /locus_tag="VIV63_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEY6126484.1"
                     /translation="MQEVLPDTPGASFTGAPLMSRRFRQFLPVVIDVETGGFNCVTDA
                     LLEIAAVMIEFGPDGRLRRGESHAFHVRPFEGANLEPASLAVNGIDPFHPLRPAIPEK
                     DALGRVFTEVRAAMKAQDCRRAVLVGHNAAFDLGFLNAAVGRADIKRNPFHPFSCFDT
                     ATLAGAAFGQTVLAKAAQVCGIAWDSSEAHSARYDAERTADIFCEVCNALDSVWRKSE
                     VRAQALWNSGEPPGDTPHLPE"
     gene            complement(3636..3962)
                     /gene="grxD"
                     /locus_tag="VIV63_17660"
     CDS             complement(3636..3962)
                     /gene="grxD"
                     /locus_tag="VIV63_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY6126485.1"
                     /translation="MAEDVVSRIKTHIGTSPAVLFMKGTPDFPQCGFSAQVVSALRAS
                     EAGFHAVNIFEDPELRDALKQFSNWPTYPQLYVNGELVGGCDIVLDMHRSGELKKLLT
                     EAGATA"
     gene            4211..4822
                     /locus_tag="VIV63_17665"
     CDS             4211..4822
                     /locus_tag="VIV63_17665"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY6126486.1"
                     /translation="MKIFTNHRYALITGIVLAVVVSLAIDVGFDGQITFQAQSLARWV
                     HIVAGVFWIGLLYYLNAVQVPAMATATEDKGGPGPAAIGKYVAPRALFWFRWSALVTW
                     LAGAWLLNKNFIHAFSFGTTVEGGSLYHVVIGVGAWLGTIMLLNVWGIIWPNQKKVLG
                     IVPATDEEKAKARKTALYASRTNFILSFPMLLCMGGATHGLPF"
BASE COUNT          874 a         1549 c         1496 g          940 t
ORIGIN      
        1 aggccttcca tgccgaaggt gcgcgcttcg tcgggcacga tgggcacgac gttcttgccg
       61 atgttcttgt ccttgagcag catcgtgagg atgcgcacga aggccatggt ggtgctgatc
      121 tcgcggtcgc ccgtgccttc cagcatcgcg ctgaacgccg acagctcggg gatgaccagc
      181 ggcttcgcca cgatcttgcg tgcgggtaga tagccaccga gcgccttgcg gcgttcgtgc
      241 aggtacttca tctccggcga gtcgtcggcc ggcttcatca gcgccagttt gcccacctgc
      301 tcgtcggtga gcgggatgtt gaaacggtcg cgcacggcac gcagcgcttc ttcgtcgagc
      361 ttcttctgct ggtgagccac catctggcct tcggccgcct tgccgagacc gaagcccttg
      421 acggtcttcg cgagaatgat ggtcggctga cccttgtgat tcatggccgc cgagtaagcc
      481 gcgaacacct tgcgcgcgtc gtggccgccg cggttcaggc gccagatgtc gtcatccgac
      541 atgttggcga ccatctcctt gagctcggga tacttgccga agacgttctc gcgcgtgtac
      601 gcgccgccct tggccttgaa gttctggtac tcgccgtcga cgcactcttc catcaggcgt
      661 tgcaggagac cctgcgtgtc gcgcgcgagc agcggatccc agcgcgaacc ccacaccacc
      721 ttgatgacgt tccagcccgc gcccatgaat gcggcttcga gctcctggat gatcttgccg
      781 ttgccgcgta ccgggccgtc gagccgctgc aggttgcagt tgacgacgaa caccaggttg
      841 tcgagcttct cgcgtaccgg cagcgtgagc gcgcccatgc tttccggctc gtccatctcg
      901 ccgtcgccga ggaaggccca gaccttgcga tcggtgaccg gcaccatctc gcggttttcc
      961 atgtagcgct gaaagcgcgc ctggtagata gccatcatgg gtccgagacc catcgacacc
     1021 gtcgggaact gccagaagtt aggcatgagc cacggatgcg gataggacga caggccgtcg
     1081 ccactcgctt cctggcggaa atgtttgagc tggtcttcgc tgagccggcc ttcgaggaag
     1141 gcgcgtgcgt agatgccggg cgaggcgtga ccctggacga acaccatgtc gccgggatgg
     1201 tccgccgacg ctgcgcgcca gaaatgattg aagcccactt cgtacagcgt ggccgacgat
     1261 gcgtaggtag aaaggtggcc gccgtattcg ctcgacacct tgttggcatt gagcaccatg
     1321 gccagcgcgt tccagcggat gcaggcctcg atgcgcttct cgaattcgcg gttaccggga
     1381 taagacggct cgtggccggg tgaaatcgtg ttgacgtagg ccgtgttcgg acggaacggc
     1441 agatatgccc ccgaacgccg cgtgtagtcg atgagctgtt cgagcaggta gtgcgcgcgt
     1501 gccgcgccct gcgtcttcag cacggagtcg atcgattcga tccactcgcg cgtctcgctg
     1561 gggtcgaggt cttcgagctt catgatttcc gtcatacaca tccactgccg ggggtcggca
     1621 aagcaaagag agcggtgcgc gctgtgctag catgcgccgc gttgaaaatg aggtgagggg
     1681 ggcgggaaaa caaggcgcga tatgatcgtg gcttcgcctc tcccggtcaa gcaaacccca
     1741 atccgcacag caggttacgg cgcgttttgc gcccttcact attcgtggaa atcgaaatcc
     1801 gccgccctga cgactggcac ctccatctgc gcgacggcgc ggccatggcg gccgtgctgc
     1861 cgtttaccgc cgagcgcttc gcacgcgcca ttgtgatgcc gaatctgaag ccgccggtca
     1921 ccacggtggc gtcggcagag gaatatcgcc agcgaatcct cgcggcgctg cccgccggat
     1981 cggccttcac gccgctcatg acgctctatc tcacggatca gaccgatccg gcggagatcg
     2041 acaaggcctt gaaatcgcag tgcgtgtacg cctgcaagct ctatccggcc ggagccacga
     2101 ccaattcgga ttccggcgtg accgacatca ggcgcatcga cgacgtgctt gcgcgcatga
     2161 gcgacgcggg aatgccactt ttggtgcacg gcgagatgac ggcgcgccag attgacgtgt
     2221 tcgatcgcga aacgcggttc atcgacgaag tcctcattcc gacgctcgcg cgatttccga
     2281 aacttcgcgt ggtcttcgaa cacatcacca cggcgcgcgc cgccgaattc gtccaaggcg
     2341 cgcgggacgg tgtcgcggca acgatcacgc cacagcatct gctgcacaat cgcaacgcca
     2401 tcttcgaagg tggcattcgt ccgcactact actgtctgcc catcttgaag accgaaccgg
     2461 atcgcgaagc gctgctcgcc gccgccacca gcggcaatcc gcgtttcttt ctcggcaccg
     2521 acagcgcgcc gcacgcgcag cacaccaagg aaaacgcctg cggctgcgcc ggcatgttct
     2581 ccgcgcacgc gggcatcgag ctgtacgccg aggctttcga gtcggtgggc aaactcgaca
     2641 agctcgaagg ctttgcttcg gatttcggcg cggacttcta cgggctgccg aaaaattcca
     2701 cacgcatccg gctcgcgaaa cgcgaatggc gtccgccggc tagctaccca ttgggcaaaa
     2761 ctgacaaaga cggccggctc gtgcccatgc gcgccaatga agccatcccc tggcaactgg
     2821 tgagctgatg caagaagtcc tgccggatac cccaggcgcc agtttcacgg gcgcgcccct
     2881 catgagccgg cgcttccgcc agttcctgcc ggtggtcatc gacgtggaaa ccgggggttt
     2941 caactgcgtc accgacgcgt tgctggaaat tgccgccgtc atgatcgagt tcggcccgga
     3001 cggacggctg cgccgcggcg aatctcacgc ctttcacgtg cggccgttcg aaggcgcgaa
     3061 cctcgagcct gcttcgctcg cggtgaacgg catcgatcca ttccatccac tgcggccggc
     3121 gattcccgaa aaagatgcgc tggggcgcgt tttcacggaa gtgcgcgccg caatgaaagc
     3181 gcaggattgc cgacgcgccg tgctggtcgg tcacaacgcc gcgttcgacc tcgggtttct
     3241 gaatgcggcc gtgggacgcg cggatatcaa gcgcaatccg ttccacccgt tctcgtgctt
     3301 cgataccgcg acccttgccg gcgcggcatt cgggcagacg gtgctcgcca aggccgcgca
     3361 ggtctgcggc attgcctggg attcgagcga ggcgcactcg gcgcgttacg acgccgagcg
     3421 caccgccgac atcttctgcg aagtgtgcaa cgcgctcgac agcgtctggc gcaagtcgga
     3481 agtgcgcgcg caggctctct ggaatagcgg cgagccgcct ggggacactc cccatttgcc
     3541 ggagtaagca gtcgatcgac tggcgcagac gcgcaaaagg ggccgcacct ccttgaggtg
     3601 cggccccttt tgctaggaaa tggggagtgt ccccattagg ccgtggcgcc ggcctcggtg
     3661 agcagcttct tgagctcgcc gctacggtgc atgtcgagca cgatgtcgca gccgccgacc
     3721 agttccccgt tcacgtagag ctgcgggtag gtaggccagt tcgaaaactg cttgagcgcg
     3781 tcgcgcagct cggggtcttc gaaaatgttc acggcatgga agcccgcctc cgaagcacgc
     3841 agcgcggaga caacctgagc cgaaaaacca cactgcggaa aatccggcgt gcctttcatg
     3901 aacagcaccg cgggactggt tccgatatgg gtcttgatac gactgactac gtcttctgcc
     3961 actgtctttc tcctttgatc gagcgtccgg aggagcgccc attgcgcttc cgcgctcata
     4021 accggccacg ccgcgatttc atccaccgta cgcaaacaac ccgcgcagcg gtctttggcg
     4081 accagtacac agactttgat gcagggtgat ggcgggcgtt caatcattag tgtccggccc
     4141 acgcggagcg cgttgaatta tgggggctcg tcccctattc ttcaaaccgc tcaccgggga
     4201 gattaagaca atgaaaatat tcacgaatca tcgctacgcg ctcatcaccg gcatcgtcct
     4261 tgccgtcgtc gtctcgcttg ccattgacgt cgggttcgac ggccaaatca ccttccaggc
     4321 ccaatctctg gcgcgctggg tgcacattgt ggccggtgtg ttctggatcg gcctgttgta
     4381 ttacctcaat gccgtccaag tcccggccat ggcaacagcc accgaagaca agggtggccc
     4441 cggtccggca gccatcggca aatacgtcgc cccacgcgcg cttttctggt ttcggtggtc
     4501 agcgctcgtt acctggctcg cgggcgcctg gctcctcaac aagaatttca tccatgcgtt
     4561 ctcgttcggc acgaccgtgg aaggcggaag cctgtaccac gttgtgatcg gcgtgggcgc
     4621 gtggctcggc accatcatgc ttctcaacgt ctggggcatc atctggccta atcagaagaa
     4681 agtcctcggc atagtgccgg cgacggacga agagaaggcc aaagcgcgaa agactgcgct
     4741 atacgcatcg cggaccaatt tcatcctctc gttcccgatg ctgttgtgca tgggtggtgc
     4801 cacgcacggc ctgccgttct gattcggaaa gtcgacagcg aactcatagc gcggcaggt
//
LOCUS       DATDEU010000741         3677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_287837, whole genome shotgun sequence.
ACCESSION   DATDEU010000741 DATDEU010000000
VERSION     DATDEU010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287837"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VIV63_17670"
     CDS             <1..290
                     /locus_tag="VIV63_17670"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEY6126487.1"
                     /translation="PMRFHDSMRVTQPLAAYGLRREHLERRLYLRDEHGRIQSGFPAV
                     LAIWARLPGYRRLVGIFSWPPLLALCETIYDHLVAPGLAYWARVRQEGART"
     gene            287..643
                     /locus_tag="VIV63_17675"
     CDS             287..643
                     /locus_tag="VIV63_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126488.1"
                     /translation="MNETSALITVIVISIALSSATVFAIYQPLRKLLEAVCPLGFTAE
                     FWTRAAVTVIYLMPLWVVLVWGLPDLQRIDYYSAGEVARRGLAAASFALVAIVIATGL
                     RLSGLRAPSNYDAPVR"
     gene            complement(645..2060)
                     /locus_tag="VIV63_17680"
     CDS             complement(645..2060)
                     /locus_tag="VIV63_17680"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6126489.1"
                     /translation="MTTAKIHIEFPGRIVFIGFGSIGQGTLPLVLRHIGIPKERITII
                     TGDDRGRKVAESFGVKFVVLPLTRDNFRRELDQYIGRGDFVLNVSVDVSSIALIKFCW
                     SKGAMYLDTCIEPWPGGYTDATIPHAKRTNYALREEALALRATNARAPTAVITHGANP
                     GLVSHFVKQALLNIAADSGVNAGSPKSKDDWAQLSRTLGIKVIHIAERDTQLASKPKE
                     VNEFVNTWSVDGFVSEGQQPSELGWGTHEKNFPRDGKRFDFGCGAAIYLMQPGAATRV
                     RTWTPKAGHFFGFLITHGEAISIADYLTVRQSSEAVYRPTVHYSYHPSDSAIVSLHEF
                     AGRNWHLQDRQRILLDEITSGHDELGVLLAGHKKNAYWYGSRLNIDEARKLAPHNNAT
                     SLQVTVSCLSGMIWAMENPNAGLVEPDEIDYVRNLEICMPYLGTVGGTYSDWTPLHDR
                     ERLFPEDLDKTDPWQFKNVRV"
     gene            complement(2141..2812)
                     /locus_tag="VIV63_17685"
     CDS             complement(2141..2812)
                     /locus_tag="VIV63_17685"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6126490.1"
                     /translation="MAIDVYWGSGSPYSWRVLLALEHKGLEYRSHVLSLAMQEHKTPN
                     MMAMNPRGRVPVLKDNDYVVFESLAVLYYLDRKYPAKPLFGDSPEEGAVIMRVCCEFQ
                     AYIEPHVMRITRALLNRIGDPHSDESTYAMHMIAGEARTIEARLSKGPWIVGEKAGAS
                     DYMIYPGIKLLLRALALPEARELSSRFLPLESNYPALGRWLQRVESLPGFEKTFPPHW
                     RDATS"
     gene            2839..>3677
                     /locus_tag="VIV63_17690"
     CDS             2839..>3677
                     /locus_tag="VIV63_17690"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY6126491.1"
                     /translation="MHHFTTLIDTNTLSEQLSREDLTLFDCRFDLGNTQWGEAEFARA
                     HLPGAIYLHLDRDLSGPVSGSSGRHPLPDPQRFVERLAALGVNPDSQIVAYDQGNAAY
                     AARLWWLARWVGLRHVAVLDGGFAAWRAAGLPLDTALRTPRPGTLPLILAADAWVSSD
                     SVDELRLRPGNLLIDARGAERFAGRNETLDPVAGHVPGARNRPFLENLGADGRFLTAE
                     VLRQRFGTLLGSVPSTGAIAMCGSGVTACHNLLALEHAGLTGARLYAGSWSDWIRDPR
                     RPIA"
BASE COUNT          612 a         1188 c         1184 g          693 t
ORIGIN      
        1 tgccgatgcg cttccacgat tcgatgcgcg tgactcagcc gctggccgcc tacggactgc
       61 gccgcgagca cctcgagcgc cgactctatc tgcgcgatga gcacgggcgc atccagagcg
      121 gatttcccgc cgtgctcgcg atctgggccc gactgcccgg ataccgccgc ctggtcggga
      181 ttttctcctg gccgccgctg ctcgcgctct gcgaaacgat ttacgaccac ctggtagcac
      241 cagggctcgc gtattgggcg cgagtccgcc aagaaggagc aagaacatga acgagacttc
      301 cgccctcatc accgtcatcg tgatttccat cgcgctcagc agcgccacgg tgttcgcgat
      361 ctaccagccc ttgcgcaagc tgctcgaggc ggtctgcccc ctcgggttca cggcggaatt
      421 ctggacgcgc gccgcggtga ccgtgatcta tctcatgcct ctctgggtgg tgctggtgtg
      481 gggcttgccc gacctgcagc gcatcgacta ctactccgcg ggcgaggtcg cgcgccgcgg
      541 cctggccgcg gcgtccttcg cactggtcgc catcgtcatc gccacggggt tgcggctctc
      601 cggcctccgc gccccgtcta actacgacgc cccggtgcga tgaatcacac gcgcacgttc
      661 ttgaactgcc acggatcggt cttgtcgagg tcttccggga aaagccgctc gcgatcgtgc
      721 agcggcgtcc agtcgctgta ggtgccgccg accgtcccga gatatggcat gcagatctcg
      781 aggttgcgca cgtagtcgat ctcgtccggc tcgacgaggc ccgcgttcgg gttctccatc
      841 gcccagatca ttcccgacag gcacgacacg gtgacctgca ggctggtggc gttgttgtgc
      901 ggcgcgagtt tgcgcgcttc gtcgatgttc agtcgggatc cgtaccagta ggcgtttttc
      961 ttgtggccgg cgagcagcac gccgagttcg tcgtggccgc tcgtgatctc gtcgagcagg
     1021 atgcgctggc ggtcctgcag atgccagttg cgaccggcga actcgtgcag tgagacgatg
     1081 gccgaatcgc tcgggtgata cgagtagtgc accgtcggcc ggtatacggc ttcggaactc
     1141 tgccgcaccg tcaggtaatc ggcgatggaa atggcttcgc cgtgcgtgat caggaagccg
     1201 aagaaatgac cggccttggg cgtccaggtg cgcacgcgcg tggcagcgcc gggctgcatg
     1261 aggtagatag ccgcgccgca accgaagtcg aaacgcttgc cgtcgcgcgg aaagttcttt
     1321 tcgtgcgtac cccagccgag ctcggaaggc tgctggcctt cgctcacgaa gccgtcgacc
     1381 gaccatgtgt tgacgaactc gttgacttct ttcggcttcg acgcgagctg agtgtcgcgc
     1441 tcggcgatgt ggatgacctt gatgccgagc gtgcgcgaga gctgcgccca gtcgtccttc
     1501 gacttgggcg aaccggcgtt gacgccggaa tcggcggcga tgttgagcag cgcctgcttg
     1561 acgaaatggg acaccaggcc cggattggcg ccgtgcgtga tgaccgccgt cggcgcgcgc
     1621 gcgttggtgg cgcgcagcgc cagcgcttct tcgcggagcg cgtagttggt gcgcttggcg
     1681 tgcggaatgg tcgcgtccgt gtaaccgccc ggccatggct cgatgcaggt gtcgagatac
     1741 atcgcgccct tcgaccagca gaacttgatc agcgcgatcg acgagacgtc gaccgacacg
     1801 ttgagcacga agtctccacg gccgatgtac tggtcgagct cacggcggaa attgtcgcgc
     1861 gtgagcggca ggaccacgaa cttgacgcca aaagattcag cgactttcct gccgcgatcg
     1921 tccccggtga tgatcgtgat gcgctctttc gggatgccga tgtggcgcag gacgagtggc
     1981 agcgtgccct ggccgatgct gccaaaaccg atgaaaacga tgcgacccgg aaactcgatg
     2041 tgaatcttgg ctgtagtcat gattaccccg tgcaaatcag ggcgtgaggt tatcaaccat
     2101 catccggcgt tgccacccga cgcggtgaca acctgtcacg tcaggaagtg gcgtcgcgcc
     2161 aatgcggcgg gaaggtcttc tcgaagccgg gcaaggattc gacgcgttgc agccagcgcc
     2221 cgagcgcggg gtaattggat tcgagcggca ggaagcgcga cgacaactcg cgcgcttccg
     2281 gaagcgcgag cgcacgcagc agcagtttga ttcccgggta gatcatgtag tcggaagcgc
     2341 ccgccttctc cccgacgatc cacggaccct tggacaggcg agcctcgatg gtgcgcgctt
     2401 cgccggcgat catgtgcatg gcgtacgtgc tttcgtccga gtgtggatcg ccgatgcgat
     2461 tcagcaatgc gcgcgtaata cgcatgacat gcggttcgat gtacgcctgg aactcgcagc
     2521 acacccgcat gatgaccgcg ccttcttccg gcgaatcgcc aaacaacggt ttcgccggat
     2581 atttgcggtc gaggtagtag agaacggcca gcgactcgaa caccacgtag tcgttgtcct
     2641 tcaggaccgg cacgcggccg cgcgggttca tggccatcat gttcggggtc ttgtgctcct
     2701 gcatcgccag cgagagcacg tggctgcggt actcgagccc cttgtgctcg agcgccagca
     2761 gcacccgcca cgaataagga ctgccacttc cccaataaac gtcaatggcc atgagtgatc
     2821 cggattgtaa aatgcggcat gcaccatttc accacgctca tcgacacgaa cacactctcc
     2881 gaacaacttt cgcgcgagga cctcaccttg ttcgattgcc gcttcgacct cggcaacaca
     2941 cagtggggcg aggcggagtt cgcgcgcgcg catctgcccg gcgccatcta cctgcacctc
     3001 gatcgcgacc tgtccggtcc cgtgtccggg agcagcggcc gccatccgct accggatccg
     3061 cagcgtttcg tcgagcgcct cgcggcgttg ggcgtcaacc cggatagtca gatcgtcgcc
     3121 tacgaccagg gaaatgccgc gtatgcggcg cggctctggt ggctggcgcg ctgggttgga
     3181 ctgcgccatg tcgcggtgct cgacggtggt ttcgcggcgt ggcgcgccgc gggactgcca
     3241 ctcgacaccg cccttcgcac gccgcggccg ggcacacttc cgctgatcct cgctgcggat
     3301 gcgtgggtga gcagcgacag cgtcgacgaa ctgcggcttc gccccggcaa tctgctcatc
     3361 gacgcgcgcg gtgcggagcg attcgcgggg cgaaacgaaa ccctcgatcc agtcgccgga
     3421 cacgtgccgg gcgccaggaa tcgtcctttc ctcgaaaatc tcggcgccga cggacgattc
     3481 ctgaccgccg aagtgctgcg gcaacggttc ggcacattgc tgggttccgt gccttccacg
     3541 ggcgcgatcg ccatgtgcgg atcgggcgtc accgcctgcc acaacctgct cgcgctcgag
     3601 cacgccggac tcaccggtgc tcgtctctat gcgggttcct ggagcgactg gatccgcgac
     3661 ccccgccgtc cgattgc
//
LOCUS       DATDEU010000742         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1018677, whole genome shotgun sequence.
ACCESSION   DATDEU010000742 DATDEU010000000
VERSION     DATDEU010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018677"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2413)
                     /locus_tag="VIV63_17695"
     CDS             complement(<1..2413)
                     /locus_tag="VIV63_17695"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY6126492.1"
                     /translation="MGWQFWIDRGGTFTDIVARRPDGALLTHKLLSESTTYDDAATFG
                     IRELLGVPSGAALPTDLIDAVRIGTTVATNALLERKGDRTVFVTTRGFADALRIAYQH
                     RPKLFVRHIELPSMLYEDVIEVDERIGAHGDVIHALDSPAAETALRASYARGVRACAI
                     VLMHGYRHSAHEQQLAALAARVGFEQISVSHRVSPLMKLVARGDTTVADAYLSPVLRR
                     YVQRVAGALPGTRLQFMQSSGGLSDAQAFHGKDAILSGPAGGIVGAARASLAAGFGKI
                     IAFDMGGTSTDVGHFAGDYERALDVEVAGVRLRTPILRIHTVAAGGGSICSFDGVRLR
                     VGPESAGANPGPACYRRGGPLTVTDCNVLLGRIQPDFFPSLFGPSGDAPLDADIVRHR
                     FAELSASIPGRDTAALAAGCIRIAVENMANAIKKISVQRGHDVTEYTLTCFGGAAAQH
                     ACLVADALDMRRVFIHPLAGVLSAYGIGLADTLVMRQRTAETPLDASSLQELDAGFGE
                     LELESRGELRAQGFEPAAIEVERTVLLKYAGTDSTLTLTLQTTSTPESLTADFLTQYR
                     TRFGFHVPGRAIVIDTIGVTATGRSPQLRGAARTFAPRFDALAPIATRPVFFEGGWAD
                     TPFFERESLRPRDALEGPAIICERNTTVVVEHGWRVTLTEADDLVLDKIGAARREALS
                     TRADPVLLEIFNNLFMAIAEQMGVTLANTASSVNIKERLDFSCALFDARGQLIANAPH
                     MPVHLGSMGESVQEILRRRGRDIQPGDAFALNAPYAGGTHLPDVTVVMPVFLGAGGPA
                     FFVAAR"
     gene            complement(2466..>3096)
                     /locus_tag="VIV63_17700"
     CDS             complement(2466..>3096)
                     /locus_tag="VIV63_17700"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126493.1"
                     /translation="AGGHPADSTTDALIRLLRRGQFSDGRWVTPVRPPSEASVFTATA
                     VSMRGIQLYGNPRSAADQAAIASAAAWLRKSTPRNTEDATFRLLGLEWAHAPAEERRA
                     AIDALVAAQKPDGGWAQLDYRASDAYATGQVLVALHEAGVPVKSPAYQRGVRYLLDTQ
                     LVDGSWLVPTRTLPTQTYFESGFPHGAHQFISAAGTQWATQALAWSVRR"
BASE COUNT          508 a          985 c         1120 g          483 t
ORIGIN      
        1 cgcgcgccgc aacgaagaat gcgggaccgc ccgcgccgag gaacacgggc atgaccaccg
       61 tgacatcggg caggtgcgtg ccgccggcgt agggtgcgtt cagtgcaaat gcgtccccgg
      121 gctgaatgtc gcggccgcgc cggcgcagga tttcctgcac ggattcgccc atcgaaccca
      181 ggtgcaccgg catgtgtggt gcgttggcga tgagttgtcc gcgtgcatcg aacagcgcgc
      241 aggagaaatc gaggcgttcc ttgatgttca cggagctcgc cgtgttggcg agcgtgacgc
      301 ccatctgttc ggcgatggcc atgaagagat tgttgaagat ctcgagcagc accgggtcgg
      361 cgcgcgtgga gagggcctca cgcctcgcgg cgccgatctt gtcgagcacc aggtcgtctg
      421 cctcggtgag cgtaacgcgc catccgtgct cgacgacgac ggtggtgttg cgttcgcaga
      481 tgatggcagg accttcgagt gcatcgcgag gccgcaggga ctcgcgttcg aagaacggcg
      541 tgtcggccca gccgccttcg aagaaaaccg ggcgcgtggc gatgggcgcc agtgcgtcga
      601 agcgcggcgc gaaggttctc gccgcgccac gcaactgcgg agaacggccg gtggccgtga
      661 cgccgatggt gtcgatgacg atcgcgcggc cgggcacgtg aaagccgaag cgcgtgcggt
      721 attgcgtgag gaaatcggcg gtcaggcttt cgggcgtgct ggttgtctgc agcgtcagcg
      781 tgagcgtgga gtcggtgccc gcgtatttga gcaacaccgt gcgttcgact tcgatggcgg
      841 ccggctcgaa accctgggcc cgcaactcgc cgcgcgactc gagttcgagt tcgccgaacc
      901 cggcgtccag ctcctgcaag gagctcgcat ccagcggcgt ctcggccgtg cgctggcgca
      961 tcaccagcgt gtcggccagg ccgatgccat aggccgacaa cacgccggcg agcggatgaa
     1021 tgaacacacg gcgcatgtcg agcgcatccg cgacgaggca ggcgtgctgg gcggcggcgc
     1081 cgccgaagca cgtgagcgtg tattccgtga cgtcgtggcc gcgctgcacg gaaatcttct
     1141 tgatggcgtt cgccatgttc tccacggcga tgcggatgca gcccgcggcg agtgccgcgg
     1201 tgtcgcggcc gggaatggac gccgagagtt cagcgaatcg atgacgaacg atatcggcgt
     1261 ccagcggtgc gtcgcctgag ggtccgaaca acgacgggaa aaagtccggc tgtatgcggc
     1321 ccagcagcac gttgcagtcg gtcaccgtga gcggtccgcc gcgccggtag caggccggcc
     1381 ctggattcgc gcccgcggat tcaggcccga cgcgcaggcg cacgccgtcg aagctgcaga
     1441 tggagccgcc gcccgcggcg acggtgtgga tgcgcaggat cggcgtgcgc agccggacgc
     1501 ccgcgacttc gacgtcgagc gcacgttcgt agtcgccggc gaaatggccg acgtccgtcg
     1561 acgtgccgcc catgtcgaac gcgatgatct tgccgaagcc cgcggccagc gaggcgcgcg
     1621 cggcgccgac gatgccgccc gcggggccgg agaggatcgc gtccttgccg tgaaacgcct
     1681 gcgcatcgct caagccgccc gacgactgca tgaattgcag gcgcgtgccc ggcaacgcgc
     1741 cggcgacgcg ttgcacgtag cggcgcagca cgggcgatag ataggcgtcg gcgaccgtgg
     1801 tgtcgccacg cgcgaccagc ttcatcaagg ggctgacgcg atgggacacg gagatctgct
     1861 cgaaccccac gcgtgcggcg agcgcggcga gttgctgctc atgcgcggaa tggcggtagc
     1921 cgtgcatcag cacgatggcg caggcgcgca cgccgcgcgc gtacgaggcg cgcaacgcgg
     1981 tctcggcggc gggggaatcg agcgcgtgga tcacgtcgcc gtgtgcgccg atgcgttcgt
     2041 cgacttcgat cacatcttcg tagagcatcg acggcagttc gatgtgccgc acgaagagct
     2101 tcgggcggtg ttgataggcg atgcgcaacg catcggcgaa tccgcgcgtg gtgacgaaga
     2161 cggtgcggtc gcccttgcgt tcgagcagcg cgttggtggc tacggtggtg ccgatgcgca
     2221 ccgcgtcgat gaggtcggtg ggtagcgccg ctcccgatgg gacgccgaga agctcgcgga
     2281 tgccgaaagt cgccgcgtcg tcgtacgtgg tgctttcaga gagcagcttg tgggtgagga
     2341 gggcgccgtc ggggcgccgc gccacgatgt ccgtgaaggt gccgccgcga tcgatccaga
     2401 attgccaacc cacgcccgca gcctaatggg gacattcctg atttgctagc aaaaggggac
     2461 gcggctcagc gacggacgct ccacgccagc gcctgcgtgg cccactgggt cccggccgcc
     2521 gagatgaatt gatgcgctcc atgcggaaaa ccactctcga agtaggtctg cgtggggagc
     2581 gttcgcgttg gcacgagcca ggaaccgtcg acgagttgcg tgtcgagcag gtagcgcacg
     2641 cctcgctgat acgccggtga tttgaccggc acgccggcct catgcagcgc caccagcacc
     2701 tggcccgtgg catacgcatc actggcgcga tagtcgagct gcgcccagcc gccatccggt
     2761 ttctgcgcgg caaccagggc gtcgatcgcc gcacggcgtt cctccgccgg cgcgtgcgcc
     2821 cactcgagcc ctaacaatcg aaacgtcgca tcctcggtat tccgcggtgt cgatttgcgc
     2881 agccaggcag ccgcgctggc gatggccgcc tggtccgccg cgctgcgcgg attgccatag
     2941 agctggatgc cgcgcatcga taccgccgtc gcggtgaaca cgctcgcctc actcggcggg
     3001 cgcaccggag tcacccagcg accgtcggag aattgtccgc ggcgtagaag ccggatgagc
     3061 gcgtcggtgg tcgagtcggc gggatgaccg cctgcc
//
LOCUS       DATDEU010000743         3500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_727362, whole genome shotgun sequence.
ACCESSION   DATDEU010000743 DATDEU010000000
VERSION     DATDEU010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727362"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2190
                     /locus_tag="VIV63_17705"
     CDS             <1..2190
                     /locus_tag="VIV63_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6126494.1"
                     /translation="MFDDVGEIVEEALRRVGKRVVLALPLGIGKPNLIANEFFRRARG
                     DSTLDLTIFTALSLRKPTGQSPLEKAFIDPLAARVFGNYPDLDYLSAVRRDEVPPNVK
                     VIEFFFEPGSLLNSSHAQSHYLCANYTHVARELLARGVNVIAHVVAKRVVGGVTEISL
                     GSNPDVTIDLMPEVAKMRAAGTPVVLLGQVHREMPFMLGAAALGADQFDLLLDHSRYD
                     YDLFAPPNPSLSTVDHAIGLHASSLVRDGGTLQIGIGELGDSIVYSLLLRHQQNEAWR
                     RALADAGTERNAALVEAVGGRAPFAAGLFGATEMFVDQMLDLYRAGILRRRVYDYLPL
                     QRALAGNGTITRIDETLLEAMLAAGAGPVLSASDVAAFKAAGVFRSETRFDNGHIISP
                     EGTRIEANLGSAKVRVALARECLGLNLQGGTVMHAGFLLGPRSFYAALGALPENERRL
                     FDMRGVGYINQLYGDDLALRVAQRSHARFVNTTMMLTTLGAAVSDGLADGRVVSGVGG
                     QYNFVTMAHALPGARSVLCVRSTRRKDGRMSSNIVTSYGHTTIPRHLRDIAVTEYGIA
                     DLRGRTDAECVAAMLNIADSRFQNALLTEAKRAHKIGSDYHIPDEYRRNTPERLEEAF
                     AAQRRAGLFSEYPFGTDLTREEIDLARALRWLKENTGSKRARLRNVARALFVRPRDSE
                     RPYLTRLQLDAPEDFSSRLTAKLVRLGLRATADTRQLTTSRTAAKDP"
     gene            complement(2156..2575)
                     /locus_tag="VIV63_17710"
     CDS             complement(2156..2575)
                     /locus_tag="VIV63_17710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6126495.1"
                     /translation="MPQPFHLAIPVHSLSAARDFYGRLFGCPEGRSSDEWVDFDFFGH
                     QVVAHLDPGKTQHVHHNEVDGHDVPVPHFGVVLDWQAWQSLAERLRSLGTRFEIEPGI
                     RFAGQVGEQATMFLYDPSGNALEFKAFKDPSRLFAKS"
     gene            complement(2665..>3500)
                     /locus_tag="VIV63_17715"
     CDS             complement(2665..>3500)
                     /locus_tag="VIV63_17715"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY6126496.1"
                     /translation="ARKATKNIDGESMTGWFTEDFTLAELKTLRARERLPDLRKSNTR
                     YDGMFVIPTFDEVLDLVAAAEAQRAAAARAAGLPPPRRIGIYPETKHPSYFATLGLSF
                     DDAMLEALTRHGYGKRSDPVYLQSFEIANLKALRRRTDLSLVQLVSPSGQPFDFTLAG
                     DPRTYLDLMNDPGLREIAAYADAIGPHKGMVVQFGAEGARDTGLARRARAAGLGIHVW
                     TLRAENEFLPVVLRSAGDSAAHGNLAGEIQALLDAGITGFFSDHPDIAVHARDDWWGR
                     Q"
BASE COUNT          604 a         1150 c         1151 g          595 t
ORIGIN      
        1 atgtttgatg atgtcggaga aatcgtggaa gaggcgctgc ggcgcgtagg caagcgggtg
       61 gtgctggcgc tgccgctcgg aattggcaaa cccaatctga tcgccaacga attcttccgc
      121 cgtgcgcgcg gcgactccac gctggacctc accattttca ccgcgctgag cctgcgcaag
      181 cccacggggc aaagcccgct cgagaaagct ttcatcgacc cgctggcagc gcgcgtcttc
      241 gggaattacc cggatctcga ctacctgtcc gccgtccggc gcgacgaagt tccgccgaac
      301 gtgaaggtca tcgaattctt cttcgaaccg ggttcgctgc tgaattcctc gcacgcgcaa
      361 tcgcattacc tgtgcgcgaa ctacacgcac gtggcgcgtg agctcctggc ccggggcgtg
      421 aatgtgatcg cgcacgtggt cgcgaaacgc gtcgtgggag gcgtcaccga gatcagtctc
      481 ggcagcaacc cggacgtcac catcgatctc atgcccgaag tggcgaagat gcgtgcggcg
      541 ggcacgccgg tggtgctcct cggccaggtg catcgcgaga tgccgttcat gctgggagcg
      601 gctgcgctgg gcgccgacca attcgatctg ctgctcgacc actcgcgtta cgactacgac
      661 ctgttcgcgc cgccgaatcc gtcgctttcc acggtcgatc acgccatcgg actgcatgcg
      721 agcagcctgg tgcgcgatgg cggcacgctg cagatcggca tcggcgagct tggcgactcc
      781 atcgtctatt cgctgctgct gcgtcaccag cagaacgaag cgtggcgccg tgcgctggcc
      841 gacgccggca ccgaacgcaa cgcggcgctg gtcgaagcgg tcggaggccg ggcgccgttt
      901 gccgccggac tgttcggcgc caccgaaatg ttcgtcgacc agatgctcga cctgtaccgc
      961 gcgggcattc ttcgccgccg cgtctacgac tatctaccgc tgcagcgcgc gctggccggg
     1021 aacggcacca tcacgcgtat cgatgaaaca ctgctcgagg ccatgctggc cgcgggcgcg
     1081 gggcccgtgc tctcggcgtc cgacgtggcg gcgttcaagg cggccggcgt tttccgcagc
     1141 gagacgcgct tcgacaacgg ccacatcatc tcgcccgagg gcacgcggat cgaagccaac
     1201 ctcggcagtg ccaaggtgcg cgtggcgctg gccagggaat gcctggggct caacctccag
     1261 ggcggcacgg tgatgcacgc cggcttcctg ctgggcccgc gctcttttta tgccgcgctc
     1321 ggcgcattac ccgaaaacga gcggcgcctc ttcgacatgc gaggcgtggg ctacatcaac
     1381 cagctatatg gcgacgacct cgcgctgcga gtcgcgcagc gcagtcacgc gcgtttcgtg
     1441 aacaccacga tgatgctgac gacgctgggt gcggcggtgt ccgacggcct cgccgatggc
     1501 cgcgtggtgt cgggcgtcgg cgggcagtac aacttcgtca ccatggcgca tgcactgccc
     1561 ggggcgcgtt cggtgctctg cgtgcgctcg acgcgccgga aagacggcag gatgagctcg
     1621 aacatcgtca cgagttatgg gcacaccacc attccgcgcc atttgcgcga catcgccgtc
     1681 accgaatacg gcatcgccga cctgcgcggc cgcaccgacg cggaatgcgt ggccgccatg
     1741 ctcaacatcg ccgactcgcg tttccagaac gcgttgctga cggaagcgaa acgcgcgcac
     1801 aagatcggct ccgactacca catccccgac gagtatcgcc gcaacacccc ggagcggctc
     1861 gaggaagcct tcgccgcaca gcgacgtgcc gggctcttct cggagtatcc cttcggcacg
     1921 gatctgaccc gcgaggagat cgatctcgcg cgcgcgctgc gctggctgaa agaaaacacg
     1981 gggagcaagc gagctcgtct gcgcaacgtc gcccgggcac tgttcgtgcg gccgcgcgac
     2041 agcgaacgtc cctatctcac gcggctgcag ctcgacgcgc ccgaggattt ttcctcgcgc
     2101 ctgacagcga agctggtgcg tctgggttta cgtgccacgg cggacacgcg gcaactcacg
     2161 acttcgcgaa cagccgcgaa ggatccttga acgccttgaa ctcgagcgca ttgcccgacg
     2221 ggtcgtagag gaacatggtg gcctgctcgc cgacctggcc cgcgaagcgg atacccggtt
     2281 cgatttcgaa gcgcgtgccc agcgaacgca gtcgctcggc gagcgactgc cacgcctgcc
     2341 aatcgagcac cacgccgaag tgcggcacgg gcacatcgtg cccgtcgacc tcgttgtgat
     2401 ggacgtgctg cgtcttgccg gggtcgaggt gcgcgactac ctggtggccg aaaaaatcga
     2461 agtccaccca ttcatcgctg ctgcgccctt cggggcagcc gaacaaccga ccgtagaaat
     2521 cgcgcgccgc gctcagcgaa tgaaccggga tcgcgagatg aaacggttgc ggcatcgatc
     2581 agatgagttg cgcggccgcc tgccacagga cgagggcgac gcaaagagca cgtgtgcagc
     2641 gttcgatcat tttcgttctc ccatttattg cctgccccac cagtcgtcgc gcgcatgcac
     2701 ggcgatgtcg gggtggtcgg aaaagaaacc cgtgattccc gcgtcgagca gcgcctggat
     2761 ttcccctgcg agattcccat gcgccgccga gtcgcccgcc gatcttagaa caaccggaag
     2821 gaattcgttc tccgcgcgca gggtccagac gtgaattccg aggccggccg cccgggcgcg
     2881 ccgggcgagg ccggtatccc gagcaccttc cgcgccgaac tgaaccacca tccccttgtg
     2941 ggggccgatg gcatcggcgt aggcagcgat ctcgcgaagc ccggggtcgt tcatgaggtc
     3001 gaggtaggtg cgcgggtcgc ccgcgagcgt gaaatcgaag ggctggcccg aaggtgagac
     3061 gagctggacc agcgacaggt cggtgcggcg gcgcagcgcc ttgagattcg cgatttcgaa
     3121 tgactgcagg tacaccggat ccgagcgctt gccgtagccg tgacgggtca gcgcctcgag
     3181 catggcgtca tcgaagctca agccgagggt cgcgaagtag ctcggatgtt tggtctcggg
     3241 atagatcccg attcgccgcg gcggtggcaa cccggcggcg cgtgcggccg ctgcgcgctg
     3301 cgcctctgcg gcggcgacga ggtccaggac ttcgtcgaac gtcggaatga cgaacatccc
     3361 gtcgtaacgc gtgttcgact tgcgcaggtc gggcagccgt tcgcgcgcac gaagcgtctt
     3421 caactccgcg agcgtgaagt cttcggtgaa ccacccggtc atggattcgc cgtcgatgtt
     3481 cttcgtggct ttgcgcgcgg
//
LOCUS       DATDEU010000744         5824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_727458, whole genome shotgun sequence.
ACCESSION   DATDEU010000744 DATDEU010000000
VERSION     DATDEU010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727458"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VIV63_17720"
     CDS             complement(<1..1563)
                     /locus_tag="VIV63_17720"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6126497.1"
                     /translation="MVHTDFALRRPVTTLMAFAAVAVIGIVSSRLLPLEQYPDVSFPF
                     MGAGVPYPGSTPEETEELITRPIEDALATLPGIEEIRSTSSDQDARFEIRFAWGTDIN
                     TAGFEVRNKLDSVRAQLPKTADRMWMWMASTADSPMLTVRFSADQDLSSQYDLLDRYF
                     KKPIERIDGVARVELAGVEAREVRILVDPGRLAAHGVDIRQLVQLLEKSNFSVGAGQI
                     TGNGERLMVRPLGEFRSLDEIRNLVIKGNVHVGDIATVELISPEITRRRHLDGRPAVG
                     LDVFKSTQANVVEVVDRVLAVIEHNKALPQMQGIRVFTIDNQAEAIRSSLGDLTEAGL
                     IGAALAFAVLFLFLRHWPTTLIVSASVPLSLLVTLAVMYFVGLTVNVMSMMGMMLAIG
                     MLVDNAVVVTESVFRHRQLDPDHPQAATLAGVKEVGVATLAGTATCVVVFLPILFGSS
                     NQLSIWLTHAAIPICVAMIASLIIAQTLIPMVTSRFPAPPPLDEKSWISRLQDRYTKT
                     LHWSIHHRGWTAF"
     gene            complement(1569..5009)
                     /locus_tag="VIV63_17725"
     CDS             complement(1569..5009)
                     /locus_tag="VIV63_17725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6126498.1"
                     /translation="MGLVDFAIRRRVTIAMATVAITLLGMISLSRLKVNLLPDLSYPT
                     LTIRTELPGAAPTEVENLISRPVEEAAGVVRNVRSVRSVSRSGQSDVIIEFTWGTDMD
                     FAGIEVRERLDLLWLPTEATRPLLLRFDPSSEPVMRVAFVDETALASKTGAGNHEDRL
                     KFLRRFADDRIKQEIESVEGSAAVKVSGGFEDEVQIYVDQQRLAQLRLSIEQVARRIG
                     AENVNLSGGRLEQGTQRFLVRTVNEFDTLDDMANAVIANVDGQPVYLKDVARVERGYK
                     DRTAITRLNGAECIELAIYKEGDANTVQLAQGITAKLTALQKNLPAGTKLVPVYDQSK
                     FIASAVSDVKDAAILGGLLSILVLYFFLRDAWATIVTGIVIPVTVVGIFVMMYAFDLT
                     LNVMSLGGIALSVGMLIDNSVVILEAIARRKEAGLSTIDAAREGTAEVATAVTASTLT
                     SVAVFFPMVFVSGIAGQLFRDQALTVTFAQLISLMVGITLVPMLTAWRARLGDKETEL
                     ASDAGEPRETLGALRRYLRATLANFNVNSIVRAPERAETSATGKARLLALPLFAIRWV
                     LRSLAWLWRYAFTFTVRVLRVVFGLLGKLFAVLLSPFVWATQVSYRFLDRRYPALLDW
                     ALTRRAAVLLTAFGLLAATALIVPRLGTELIPQLSQGEFTVKMRLPAGAPLETTDTQV
                     QVIHKAALSLPELDSAYAVAGTGNRLDANPVDSGENTGNLDVKLRAPIDKEGEERAIQ
                     ELRSRLAGIPGAQYEFTRPSLLTLATPVEVILAGYDLERLDVAASAVRTSMEASGAFK
                     DIRSSIEGGHPEIQIVFDQERASQLGLAVRDIADRVVSNVRGDVATRYRLQEKKIDVL
                     VRSVDTRAASIDEVRNLVINPGSERPVPLSAVADVRLATGPAEIRRANQERVAVISAA
                     PARGDLGDATVRAQEILAQTTLPVGIVGSVSGQSEEMTQSFRSLGLAFALAVFLVYLV
                     MASQFESLLHPFVILFTIPMGLIGSVWGLYITGTTINSVALIGLIMLAGIVVNNAIVL
                     IDAINQARERGLERIEAIKVAGRTRLRPILITSVSTIIGLIPMAIGIGEGAEIRRPMA
                     ITVIAGNLVATFLTLIVIPVLYAVLDRKEFVKKAALPGAANPLATPAMPPLALRKQED
                     "
     gene            complement(5012..>5824)
                     /locus_tag="VIV63_17730"
     CDS             complement(5012..>5824)
                     /locus_tag="VIV63_17730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6126499.1"
                     /translation="VQAGQMLAQLDDRQLRLQAAQTRAALAKAERDFNRQVELHQKGL
                     VSAGAFEGLKYDLDNQRAANDLASLSLSYTEIRAPFSGIVSARHVKLGKEIAVGTSVF
                     RVTDPTPLKAAVYIPERELARLKIGQAASIAVDALAGRSFPAIVKLVSPTVDAATATF
                     KVTLEVNDPKGDLKPGMFSRVGIVFERRAEALTIPRIALLDTDGASNVFVVSSGKAEQ
                     RAIKTGLSNAGKIEVIDGLTGAEQVVVVGQNGLKDGNPVRVVSLEQTAKTGS"
BASE COUNT         1015 a         1835 c         1991 g          983 t
ORIGIN      
        1 gaacgccgtc cagccgcgat ggtgaatgct ccagtggagc gtcttcgtgt atcgatcctg
       61 caggcgcgag atccagctct tctcatcgag cggcggcggc gccgggaagc gggaggtcac
      121 catcggaatc aatgtctgcg cgatgatcag cgatgcgatc atggcgacgc agatcggaat
      181 ggcggcatgc gtgagccaga tggagagctg gttgctcgat ccgaagagga tgggcaggaa
      241 cacgacgacg caggtggcgg taccggccag cgtcgcaaca ccgacttcct tgacgcccgc
      301 gagcgtcgct gcctgcggat ggtccggatc gagctggcgg tggcggaaca cgctttcggt
      361 aaccaccacg gcgttgtcca ccagcatgcc gatggcgagc atcatgccca tcatcgacat
      421 gacgttcacc gtgaggccga caaagtacat caccgcgagc gtgaccagca gggacagcgg
      481 cacggacgcg ctgacgatga gcgtggtcgg ccagtggcgc aggaacagga acaacaccgc
      541 gaaggcgagc gcggcgccga tgaggccggc ctcggtgaga tcgcccagcg acgagcggat
      601 cgcctcggcc tggttgtcga tggtgaagac gcggatgccc tgcatctgcg gcagcgcctt
      661 gttgtgctcg atgaccgcga gcacgcgatc gaccacttcg accacgttgg cctgtgtcga
      721 cttgaaaacg tcgagaccca ccgccgggcg gccatcgaga tggcggcgcc gcgtgatctc
      781 ggggctgatg agctccacgg tcgcgatgtc gccgacgtga acgtttccct tgatcaccag
      841 gttgcggatt tcatcgagcg accggaattc accaagcggc cgcaccatga gacgctcgcc
      901 gttgccggtg atctgcccgg cgccgaccga gaagttgctc ttctcgagta gctggacgag
      961 ctggcggatg tcgacgccgt gcgcggcgag ccggcccgga tcgacgagga tgcgcacttc
     1021 gcgcgcctcg actcccgcga gttcgactcg cgcgacgcca tcgatgcgct cgatgggttt
     1081 cttgaagtag cggtccagca gatcgtactg cgaggagaga tcctggtccg ccgagaaacg
     1141 caccgtgagc atgggcgaat cggcggtgga cgccatccac atccacatgc ggtccgcggt
     1201 cttcggcagc tgcgcgcgca cggagtcgag cttgttgcga acttcgaatc ccgccgtgtt
     1261 gatgtccgtg ccccaggcga agcggatttc gaaccgcgcg tcctgatcgc tggacgtgga
     1321 acggatttct tcgatgcccg gcaacgtcgc cagcgcgtct tcgatgggcc gcgtgatgag
     1381 ctcttcggtt tcttcgggcg tcgagccggg gtagggcacg cccgcaccca tgaacggaaa
     1441 gctcacgtcc ggatactgct cgagcggcag cagccgcgaa gacacgatac cgatgaccgc
     1501 gaccgcggcg aatgccatga gcgtggtgac gggccggcgc agcgcgaagt cggtgtggac
     1561 catggcgctc agtcctcctg cttgcgcagc gcgagcggcg gcatcgccgg ggtggccagc
     1621 ggattcgccg cgcccggcag cgccgctttc ttcacgaact ccttgcgatc gaggacggcg
     1681 tacaacaccg gaatcacgat gagcgtgagg aacgtggcga ccaggttgcc ggcgatcacg
     1741 gtgatggcca tggggcggcg gatctcggcg ccctcgccga tgccgatggc catgggaatg
     1801 aggccgatga tggtgctcac gctggtgatg aggatgggcc tgaggcgcgt gcgaccggcc
     1861 accttgatcg cttcgatgcg ctcgaggccg cgctcccgcg cctggttgat ggcgtcgatg
     1921 agcacgatgg cgttgttgac cacgatgccc gccagcatga tgagaccgat gagcgccacg
     1981 gagttgatgg tggtgccggt gatgtacagg ccccacaccg aaccgatcag tcccatcgga
     2041 atggtgaaca ggatgacgaa ggggtgcagc aacgattcga actgcgacgc catcacgaga
     2101 tacaccagga ataccgcgag cgcgaaggcg aggcccagcg agcggaagct ctgggtcatc
     2161 tcctcgctct ggccggagac cgagcccacg atgccgaccg gcagagtagt ttgagccagt
     2221 atctcctgcg cgcgaaccgt cgcatcaccc agatcgccgc gcgccggcgc cgccgagatc
     2281 accgcgacgc gttcctggtt ggcgcgacgg atctccgcgg gcccggtcgc gagccttacg
     2341 tcggccaccg cggaaagcgg cacggggcgc tcagagcctg gattgatcac gagattgcgg
     2401 acttcgtcga tggacgccgc acgcgtgtcg acgctgcgca ccagcacgtc tatcttcttt
     2461 tcctgcaggc gataacgcgt ggccacatcg ccacgcacgt tcgagaccac gcggtcggcg
     2521 atgtcgcgca ccgccagccc gagttgtgag gcgcgttcct ggtcgaacac gatctggatc
     2581 tcgggatggc cgccttcgat gctggagcgg atgtccttga acgcgccgct ggcctccatg
     2641 ctcgtgcgca ccgcgctggc ggcgacgtcg aggcgctcca gatcgtagcc ggcgaggatc
     2701 acctcgacgg gagtggcgag cgtgagcagc gacggccgcg tgaattcgta ctgtgcgccg
     2761 ggaattccgg cgagacgcga gcgtagttcc tgaatcgcgc gttcttcgcc ttccttgtcg
     2821 atgggcgcgc gcagtttcac gtcgaggttg ccggtgtttt cgcccgagtc gaccgggttc
     2881 gcgtccagcc ggttgccggt gccggccacc gcgtacgcgc tgtcgagttc gggcagcgag
     2941 agcgccgcct tgtggatgac ctgcacctgc gtgtcggtcg tctcgagcgg cgcgccggcg
     3001 ggcaggcgca tcttgacggt gaactcgccc tgggagagct gcgggatcag ttcggtgccg
     3061 agccgcggca cgatgagtgc cgtagccgcc agcaaaccga aagccgtgag cagcaccgcc
     3121 gcgcgtcgag tcagcgccca gtcgagcaac gccggatagc gccggtcgag gaagcgataa
     3181 ctcacctgcg tcgcccacac gaaaggcgag agcaagacgg cgaacagctt gcccagcaat
     3241 ccgaaaacga cgcgcagcac gcgaaccgtg aacgtgaacg cataacgcca cagccacgcg
     3301 agcgaacgga gcacccaacg gatcgcgaac agcggaagcg ccagcaggcg cgcttttccg
     3361 gtcgccgatg tttccgcgcg ctcgggcgcc cggacgatcg agttcacatt gaagttggcg
     3421 agcgtggcgc gcagatatcg gcggagcgcg ccgagcgtct cgcgcggttc gcccgcgtcg
     3481 ctcgcgagtt ccgtctcctt gtcgccgaga cgggcgcgcc acgcggtgag catgggaacc
     3541 agcgtgatgc ccaccatgag cgaaatgagc tgggcgaatg tgaccgtgag ggcctggtcg
     3601 cggaacaact gtccggcgat tccgctgacg aagaccatgg ggaaaaaaac cgccacggac
     3661 gtgagcgtcg acgccgtgac tgccgtggcc acttcggccg tgccttcgcg cgccgcatcg
     3721 atggtggaca ggccggcttc cttgcgccgg gcaatggcct cgagaatgac caccgagttg
     3781 tctatcaaca tgcccactga caacgcgatg ccgccgagtg acatcacgtt cagcgtgagg
     3841 tcgaacgcgt acatcatcac gaagatgccg actaccgtca cggggatgac gatgccggtg
     3901 acgatggttg cccacgcgtc gcgcaggaag aaatacaaca ccaggatgga cagcagtccg
     3961 ccgaggatcg ccgcgtcctt cacgtcgctg accgcgctcg cgatgaactt cgactggtcg
     4021 tagaccggca cgagtttcgt cccggcgggc aggtttttct ggagtgcggt gagcttcgcc
     4081 gtgatgccct gggcgagctg cacggtgttg gcatcgcctt ccttgtagat ggcgagttcg
     4141 atgcactcgg cgccgttcag tcgcgtgatg gcagtgcggt ccttgtagcc gcgttccacg
     4201 cgcgcgacgt ccttgaggta cacgggctgg ccgtcgacgt tggcgatgac ggcattcgcc
     4261 atgtcatcga gcgtgtcgaa ctcgttgacg gtgcggacga ggaaccgctg cgttccctgt
     4321 tccagacggc cgcccgagag attcacgttt tccgcgccga tgcggcgcgc cacctgttcg
     4381 atcgacagcc gcaactgcgc gagtcgctgc tggtcgacgt agatctgcac ctcgtcttcg
     4441 aagccgccgc tgaccttcac cgccgcggag ccttcgacgg attcgatttc ctgcttgatg
     4501 cggtcgtcgg cgaagcggcg caggaacttg aggcggtcct cgtggtttcc ggcccctgtt
     4561 ttcgatgcga gcgcggtttc gtcgacgaac gccacgcgca tcaccggctc gctcgacgga
     4621 tcgaagcgca gcagcagggg gcgtgtggct tcggtcggca gccacagcag gtcgagacgt
     4681 tcgcgcacct cgatgcccgc gaaatccatg tccgtgcccc aggtgaactc gatgatcacg
     4741 tcggactggc cggaacggga caccgagcgc accgaacgca cgttgcgcac cacgccggcc
     4801 gcttcctcga caggccgcga gatgaggttc tcgacctccg tgggcgcggc gcccggcagc
     4861 tcggtgcgga tggtcagcgt cggatacgac aggtccggga gcaggttcac cttgaggcgg
     4921 gagagggaaa tcatcccgag cagggtgatc gccaccgtcg ccatggcgat ggtgacgcgc
     4981 cggcgaatcg cgaagtccac gaggcccacg gctaactacc ggtcttggcg gtctgttcga
     5041 gcgatacgac gcgtaccgga ttaccgtcct tgagtccgtt ctggcccacc acgaccacct
     5101 gctcggcgcc ggtgaggccg tcgatgactt cgatcttgcc ggcgttcgac aggccggtct
     5161 tgatggcgcg ctgctcggcc ttgccactgc tcacgacgaa gacgttcgag gcgccatcgg
     5221 tgtcgagcag cgcgatgcgg ggaatggtca gcgcttcggc gcggcgttcg aacacgatgc
     5281 cgacgcgcga gaacatgccg ggcttcagat cacccttggg gtcgttgacc tcgagcgtca
     5341 ccttgaacgt cgcggtggcg gcatcgacgg tcggcgacac cagcttcacg atggcgggga
     5401 aactgcggcc ggcgagggcg tcgacggcga tggatgccgc ctggccaatc ttcaggcgcg
     5461 cgagttcgcg ctcggggatg tacaccgcag ccttgagcgg cgtgggatcg gtaacccgaa
     5521 acacgctggt gccgacggcg atctccttgc ctaacttcac gtggcgcgcc gagacgatgc
     5581 cggagaaggg cgcgcggatc tcggtatagg acaggctcaa cgacgcaagg tcgttggccg
     5641 cgcgctggtt gtcgaggtcg tacttgagac cttcgaacgc tcccgcggac accaggccct
     5701 tctggtggag ctcgacctgg cgattgaagt cgcgttcggc ttttgccagc gccgcgcggg
     5761 tctgcgcggc ctgcagacgc agctggcgat cgtcgagttg cgcgagcatc tggcccgcct
     5821 gcac
//
LOCUS       DATDEU010000745         3481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1602388, whole genome shotgun sequence.
ACCESSION   DATDEU010000745 DATDEU010000000
VERSION     DATDEU010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602388"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VIV63_17735"
     CDS             <1..1355
                     /locus_tag="VIV63_17735"
                     /inference="COORDINATES: protein motif:HMM:NF025870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tachylectin-related carbohydrate-binding
                     protein"
                     /protein_id="HEY6126500.1"
                     /translation="RWYDAGPADMARINDRFTNIAQTGWAQGARAANDICRNRDATSV
                     GGMFTGFEVKTAPAVGFADQRDGVFCFNRNNAAWFDSTLAEHAANGTPVGDLNNISWA
                     AAGRAATEYCRRKFYGGGGFFNGHQLGDKRGVICLGANSIMSDRIKAADSSGAVRAEA
                     ATGGKPTGAIGALSRDALVTQSATARATTGVSTAPLPVPVPVNTAPAVTMFYAVESDG
                     ALSEYRHDRPETGTAPNRFAPTSASWNSYAEAIPAGGNHFYARAQNGDLLWYQHDGVA
                     DGANAWRGPTQVGNGWQSFAKIFGGGDGVIYAIAPDGTLMWYRHAGFATGDARAWLGP
                     KKIGTGWQSFKQIFSAGQGAIYAITQDGKLMLYRHLDPQSGEMRWSGPTQVGTGWHGF
                     AQVFSTGNGVIYGLTPDGKLSYYRHLTWNAAQPTFQWVGPVAVGQSFNPGANIIPMLP
                     "
     gene            1416..>3481
                     /locus_tag="VIV63_17740"
     CDS             1416..>3481
                     /locus_tag="VIV63_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126501.1"
                     /translation="MPDKKEGPGPMRRALFQDVPYILDSIWRRSEFDLHRLGKVSLDD
                     PHALVLRPAWMCMAFILYVFVGLFAVFGPTLDAMCSVADAGFAGSARTWAGYILIAAA
                     VVAFFAVLNGTTWRLTRNAEELADEVCKSDTEKLELRKRLDKLVDSEGFKSEFLRGFA
                     GTFAEHFTGNVVKLVVAGVFLSWWERLSLLAAFLALMAGTALLIAPVLVTGATLGFVP
                     LDLASCTQEPNAELVYAQLAAFAVLVVGGWFIWRHWGARRAIVVFGASTALLAAVIAL
                     LWWKFTPALPSEAAGGFYPHVYLVVLGILLGVAALARVLAWWMFRKIPVDTCAHFVEA
                     LQKQDLLQDERPPPDVSWLRLFSAAVTGVFGHILHFLLLPAFVAFIAPSTWLVGLVIG
                     FAVVSAILLMYATLSTRWAQMLIHIDRWFLIGTPMVVSLFVIVIGIARLANFQYVTTV
                     LDAAPTGVLFIIMVMLYVAAWLFEFWINHWVGDELLGMLGDRAQSRRGFLPVGFTPDP
                     NAKPCWAEQRGRYIALHSTGRFLALGWFTRANPRKGEDVKGLAFSTFGFAELFYALGA
                     TKKGADLAHDIERRVRLYFGLINIALVIAMGVAINSAIPLPLAVQAMVNEQTATPGYS
                     ATVVDSLGERLLAQTAEGRPSIVVAASGGGTRAAVYTAVALEGIAQIDRARDVVLLSG
                     VSGGGVS"
BASE COUNT          541 a         1141 c         1150 g          649 t
ORIGIN      
        1 cccgctggta tgacgccgga cctgccgaca tggccaggat caacgatcga ttcaccaaca
       61 tcgcacagac cggctgggcc cagggcgccc gcgccgcgaa cgacatctgc cggaatcgtg
      121 acgcaacgtc cgtaggcggc atgttcacgg gattcgaagt gaagactgcg ccggccgtcg
      181 gcttcgcgga tcagcgcgac ggcgtgttct gcttcaaccg caacaacgcg gcctggttcg
      241 actccacgct cgccgagcat gccgccaacg gcacgccggt gggcgatctc aacaacatca
      301 gctgggccgc ggccggacgt gccgccaccg agtattgccg ccggaagttc tatggcggcg
      361 gcggcttctt caacggccac cagctcggcg acaagcgcgg cgtgatctgc ctcggggcta
      421 acagcatcat gtcggatcgc atcaaggcgg ccgattcgtc cggcgccgtg cgcgcggagg
      481 ccgcgaccgg tggcaagccg acgggagcca tcggcgcgct ctcgcgagac gcactggtga
      541 cgcaatccgc cacggcgcgc gcgacgactg gcgtatcgac cgcgccgctg cccgtgcccg
      601 tgccggtcaa caccgcgccg gccgtcacga tgttctatgc cgtcgagtcc gacggagccc
      661 tttccgaata tcgccacgac cgccccgaga ccggcacggc gcctaaccgc tttgcaccga
      721 cgagcgcgtc gtggaacagc tacgccgaag ccattccggc cggtgggaac cacttctatg
      781 cgagagcgca gaacggcgat ctcctgtggt atcagcacga tggcgtggct gatggcgcca
      841 acgcctggcg cggcccgacg caggtgggca atggctggca gtcgttcgcg aagatcttcg
      901 gcggcggcga tggcgtcatc tatgccatcg cacccgatgg cacgctcatg tggtatcgcc
      961 acgccggatt tgcgaccggc gatgcgcgcg catggctggg tccaaagaag atcggcaccg
     1021 gctggcagtc gttcaaacaa atattctcgg ccgggcaggg ggctatctac gccatcaccc
     1081 aggacggcaa gctcatgctc tatcgccatc tcgatccgca aagcggcgag atgcgctgga
     1141 gcgggcccac gcaggtgggc acgggctggc acgggttcgc gcaggtgttc tccaccggca
     1201 acggcgtgat ctacggactc acgcccgacg gcaagctgtc ttactaccgg catctcacgt
     1261 ggaacgcggc gcagcccacg ttccagtggg tggggccggt ggccgtgggc cagagcttca
     1321 accccggtgc caatatcatc ccgatgcttc cgtgacgatc gcgctgtaac atccccctcg
     1381 ccgagaacag aagaagagta ggcgagggag atgccatgcc agacaagaaa gaagggccgg
     1441 gcccgatgcg ccgggcgctg ttccaggatg tgccatacat tctcgacagc atctggcgac
     1501 gttccgaatt cgatctgcac cgtctgggga aagtcagtct cgacgatccg catgcgttgg
     1561 tgctgcgccc cgcctggatg tgcatggcct tcatcctcta cgtattcgtg gggttgtttg
     1621 ccgtgttcgg gccgacgctc gacgccatgt gttcggtcgc ggacgctggc ttcgcgggca
     1681 gcgcgcgaac ctgggccggc tacatcctca tcgccgcggc ggtggtcgcg ttcttcgccg
     1741 tgctcaacgg cacgacctgg cggcttacgc gcaacgccga ggagctcgcg gatgaagtct
     1801 gcaagtccga cacggagaag ctcgagctgc gcaagaggct cgacaagctg gtcgactccg
     1861 aaggtttcaa aagcgagttt ctgcgcgggt tcgccggaac attcgcggag catttcaccg
     1921 gcaacgtcgt aaagctcgtc gtcgcgggcg tcttcctgag ctggtgggag cgtctctcgc
     1981 tgctggccgc gttcctcgcg ctcatggccg gtaccgcgtt gctcatcgcg cccgtgctgg
     2041 tcaccggcgc cacgctcggc ttcgttccgc tggatctcgc gagctgcacg caggagccga
     2101 acgccgaact cgtgtatgcg cagctcgccg cattcgcggt gctggtcgtg ggcgggtggt
     2161 tcatctggcg gcactggggc gcgcggcgcg ccatcgtggt gttcggcgcc agcaccgcat
     2221 tgttagcggc cgtcatcgcg ctgctgtggt ggaagttcac gccggccctg cccagcgaag
     2281 ccgcgggcgg cttctatcca cacgtctatc tcgtcgtgct cggcatcctg ctgggcgtgg
     2341 cagcgctggc gcgcgtgctc gcgtggtgga tgttccgcaa gattcccgtc gacacctgcg
     2401 cgcatttcgt cgaggcgctg cagaaacagg acctgttgca ggacgaacgc ccgccgccgg
     2461 acgtgtcgtg gctgcggctg ttctcggccg ccgtcaccgg agtcttcggg cacatcctgc
     2521 attttctgct gctgcccgcg tttgtcgcgt tcatcgcgcc ttccacctgg ctcgtggggc
     2581 tggtcatcgg tttcgcggta gtcagcgcca tcctgctcat gtacgccacg ctgtcgacgc
     2641 gctgggcgca gatgctcatc cacatcgacc ggtggttcct catcggcacg ccgatggtcg
     2701 tttcgttgtt cgtgatcgtc atcggcatcg cgcggctggc caacttccag tacgtgacca
     2761 ccgtgctcga cgccgcgccc accggcgtgt tgttcatcat catggtgatg ttgtacgtcg
     2821 ccgcgtggtt gttcgagttc tggatcaatc actgggtagg cgacgagttg ttaggcatgc
     2881 tcggcgaccg cgcgcagagc cgccgcggct tcctccccgt cgggttcacc cccgacccga
     2941 atgccaaacc ctgctgggcg gagcagcggg gccggtatat cgcgctgcac agcaccgggc
     3001 gtttcctggc gctcggctgg ttcacgcgcg cgaatccgcg caagggagaa gacgtcaagg
     3061 gtctcgcgtt cagcacgttc ggattcgccg agctgttcta cgcgctcggt gcgaccaaga
     3121 aaggcgcgga tcttgcgcat gacatcgaac gccgcgttcg cctctatttc ggtctcatca
     3181 acatcgcgct ggttatcgcg atgggtgtcg cgatcaactc cgcgattccg ctgccactcg
     3241 ccgtgcaggc gatggtcaac gagcagaccg cgacgccggg ctattccgcg accgtggtcg
     3301 attcgctcgg cgagcgcctg cttgcgcaaa ccgcggaagg ccggccgtcg atcgtggttg
     3361 ccgcttcggg tggtggcacg cgcgccgcgg tgtatacggc cgttgcgctc gagggaatcg
     3421 cgcagatcga tcgcgcgcgt gacgtcgtgt tgctgtcggg cgtatcgggt ggcggcgttt
     3481 c
//
LOCUS       DATDEU010000746         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1747982, whole genome shotgun sequence.
ACCESSION   DATDEU010000746 DATDEU010000000
VERSION     DATDEU010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747982"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VIV63_17745"
     CDS             <1..595
                     /locus_tag="VIV63_17745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6126502.1"
                     /translation="GNDLHDGLGQELTGIALLLRGLENRAEREAPALSPSIEEVALLV
                     NDAIFTTRALARGLSPVTFDRGGLALALEELARRLSAMFHIDVRCVADAALDHGLESV
                     NALHLYRIAQEAVTNAAQHGHAERVQISLCLDGGRGLLRIEDNGAGFNPATLMSKGLG
                     LRIMHYRAQMMAGSLRIESARGNGTVVSCWFPRGEVN"
     gene            complement(561..2099)
                     /locus_tag="VIV63_17750"
     CDS             complement(561..2099)
                     /locus_tag="VIV63_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY6126503.1"
                     /translation="MMKVIKQQDLIDSVSDALQYISYFHPPDYIRALGKAYELEESPA
                     ARDAIAQILTNSRMCAEGRRPICQDTGIVMVFAKVGMNVRWEAAMSFEDMINEGVRRA
                     YLHPDNKLRASIVADPNFSRKNTKDNTPAVIHTQLVAGDELEITVAAKGGGSENKSKF
                     VMLNPSDSLTDWVLKTVPLMGAGWCPPGMLGIGIGGSAEKAMLLAKEALMEPIDMAEL
                     KARGPQSKTEELRIELYEKVNALGIGAQGLGGLSTVLDVKIFEYPTHAASKPVAMIPN
                     CAATRHVHFTLDGSGPARLDPPKLSDWPKIDWAPAAGSRRVNLDALTPAEVASFKPGE
                     QLLLNGRMLTGRDAAHKRIADLFASGKGLPPGVDFRNRVIYYVGPVDPVGSEVVGPAG
                     PTTATRMDKFTEMMLEKTGLIAMVGKAERGPAGIEAIRKHRAAYLMAVGGAAYLVSKA
                     IKASRVVAFEDLGMEAIYEFTVEDMPVTVAVDANGTSVHQTGPAEWSQKIAVNSLRRA
                     GTSS"
     gene            2166..>2617
                     /locus_tag="VIV63_17755"
     CDS             2166..>2617
                     /locus_tag="VIV63_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6126504.1"
                     /translation="MTEAKRVVVIGGGFAGLWAVRQLKRAPVEITLIDRRNHHLFQPL
                     LYQVATAGLAAPSIAAPLRHIVRKQRNVTVHMGEVLGIDTAARKVSLSDRQIPYDYLL
                     VASGATHAYFGHDQWAHFAPGLKTLDDAFLIRRRVLSAFERAEAATTAA"
BASE COUNT          417 a          862 c          848 g          490 t
ORIGIN      
        1 cggcaacgac ctgcatgacg gcctgggcca ggagctcacc ggcatcgccc tgctgttgcg
       61 cggactcgag aatcgcgccg agcgcgaggc accggcgctc tcaccctcga tcgaggaagt
      121 cgcgctgctg gtcaacgacg ccatcttcac gacgcgcgcg ctggcgcgcg gtctgtcgcc
      181 cgtcaccttc gaccgcggcg gactcgcgct cgcgctcgag gaactcgcgc ggcggctttc
      241 ggcgatgttc cacatcgacg tgcgatgcgt cgcggacgcg gcgctggatc acggcctgga
      301 atcggtgaat gcgcttcacc tgtaccggat cgcgcaggag gcggtgacca acgcggctca
      361 acatggccat gcggaacggg tgcagatatc gctgtgcctg gacggaggcc gcggcctgct
      421 gcgcatcgag gacaacggcg cgggattcaa cccggccacc ctgatgtcca agggcctcgg
      481 cctgcgcatc atgcattacc gcgcgcagat gatggccggt tcgctgcgca tcgagagcgc
      541 acgcgggaat ggcaccgtcg tcagctgctg gttcccgcgc ggcgaagtga attgaccgcg
      601 atcttctgac tccactccgc gggaccggtc tggtggaccg acgtgccgtt cgcatcgacg
      661 gcgacggtga ccggcatgtc ctccacggtg aactcgtaga tggcctccat gccgagatct
      721 tcgaaggcca ccacgcgcga cgccttgatg gccttcgaca cgagatacgc cgcgccgccc
      781 accgccatca gataggccgc gcgatgcttg cgaatggctt cgatgccggc gggaccgcgt
      841 tcggccttgc ccaccatcgc gatgagtccc gtcttctcga gcatcatttc ggtgaattta
      901 tccatgcgcg tggccgtcgt cgggcccgcg ggtcccacga cctcgctgcc caccggatcg
      961 accggaccga cgtagtagat gacgcggtta cggaaatcga cgcccggcgg cagacctttc
     1021 ccgctggcga agaggtccgc gatgcgtttg tgggcggcat cgcggccggt gagcattcgt
     1081 ccgttgagca acagctgctc gcccggtttg aagctcgcca cttccgcagg cgtcagggcg
     1141 tcgagattca cccggcgcga accggcggcc ggcgcccagt cgattttcgg ccagtcggac
     1201 agtttcggcg gatcgagccg cgcgggcccg ctgccatcga gcgtgaaatg cacgtgccgc
     1261 gtggccgcgc agttggggat catggccacc ggcttggacg ccgcgtgggt cggatactcg
     1321 aagatcttca cgtcgagcac ggtagatagt ccgccgagac cctgcgcgcc gatgccgagc
     1381 gcattgacct tttcatagag ctcgatgcgc agctcctcgg tcttcgactg cggtccgcgc
     1441 gccttgagct ccgccatgtc gatgggctcc atgagcgctt ccttggcgag cagcatggcc
     1501 ttttcagcag acccgccaat gccgatgccc agcatgccgg gcggacacca tcccgcgccc
     1561 atgagcggca cggtcttgag cacccagtcc gtgagcgaat cggagggatt cagcatcacg
     1621 aacttcgatt tgttctccga accaccgccc ttggccgcga cggtgatctc gagttcgtcg
     1681 cccgccacca gctgcgtgtg gatgacggcc ggcgtgttgt ccttggtgtt cttgcgcgag
     1741 aagttggggt ccgcgacgat cgacgcgcgc aacttgttgt ccgggtgtag ataggcgcgc
     1801 cgcacgccct cgttgatcat gtcctcgaaa ctcatggcgg cttcccagcg gacgttcatg
     1861 cccaccttgg cgaacaccat gacgattccc gtgtcctggc agatggggcg gcgcccttcg
     1921 gcgcacatgc gcgaattggt gagtatctgc gcgatggcgt cgcgcgcggc cggcgactcc
     1981 tcgagttcgt aagccttgcc cagcgcgcgg atgtaatccg gcggatgaaa gtacgagatg
     2041 tactgcagag cgtccgagac gctgtcgatg aggtcctgtt gtttgatgac tttcatgatc
     2101 ccgacttcat ggcttgagta cattttagcc cggcgcgcgt gtgccgtttc ggttaagttt
     2161 tccccatgac agaagcaaag cgagtcgtgg tcatcggcgg tgggtttgcc ggtttgtggg
     2221 cagttcggca gctcaagcgg gcgccggtcg agatcacgct gatcgatcgc cgcaaccatc
     2281 acctgttcca gccactgctg taccaggtgg ccaccgccgg cctcgcggcg ccctcgatcg
     2341 ccgcgccgct gcgccacatc gtgcgcaaac agcgcaacgt cacggtacac atgggcgagg
     2401 tgctcggaat cgacactgcc gcgcgtaagg tgtcgctgtc cgatcgccag attccctacg
     2461 actatcttct ggtcgcgagt ggcgcgaccc acgcgtactt cgggcacgat cagtgggcgc
     2521 acttcgctcc tggcctgaag acgctcgacg atgccttcct gatccggcgg cgcgtgttgt
     2581 ccgcgttcga acgcgcggag gccgccacca cggcggc
//
LOCUS       DATDEU010000747         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1893570, whole genome shotgun sequence.
ACCESSION   DATDEU010000747 DATDEU010000000
VERSION     DATDEU010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893570"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /gene="aat"
                     /locus_tag="VIV63_17760"
     CDS             complement(<1..618)
                     /gene="aat"
                     /locus_tag="VIV63_17760"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY6126505.1"
                     /translation="MNKTITWLKPDGDREWFPPLDQVLDEPEGLLAAGGDLSVPRLLA
                     AYRRGIFPWYSAGQPVLWWSPNPREVLYPEEFRCSRSLAKTLRNRGFVVTFDRDFAGV
                     VDACAARREHSPGTWITPEMHAAYCELHAQGHAHSVEVRLDDVLVGGLYGVLLGRMFF
                     GESMFSRERDASKVAFARLVERAVVAGLQLIDCQLPTAHLRSLGSQ"
     gene            complement(631..1785)
                     /locus_tag="VIV63_17765"
     CDS             complement(631..1785)
                     /locus_tag="VIV63_17765"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HEY6126506.1"
                     /translation="MRTSTSTNITDIDPADWDALDAGGSPFLRHAFLASLERTGCVGA
                     NTGWEPAPITLHDERGLAAAVPAYVKAHSFGEFVFDFSWAQAYAQHGLEYYPKLVLGV
                     PFTPASGARLLVRPDLDAAKTRAALIAAIHEFATARNFSSIHGLFVAGQDREAFAQAG
                     WLARRDVQFHWHNRGYRDFDHYLDSFTADKRKKAKRERRRVAEEGITFDTLLGTGIDR
                     ASLDEAYDLHRDTFLRHGHEPYLSRAFFREMPGALGDRFMIKRARAGGATVAAAVFFW
                     SPEALYGRYWGASEQHHSLHFETCYHQGIEFCIERGIQRFEPGTQGEHKVSRGFVPAN
                     TWSMHWIVDPRFRVAIGDYLRREGAHVDDYARDIENHVPYRDARRIARGD"
     gene            complement(1748..>2488)
                     /locus_tag="VIV63_17770"
     CDS             complement(1748..>2488)
                     /locus_tag="VIV63_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6126507.1"
                     /translation="RFNTEIVNDHIHTVDFSKRPFVLNGDYGSYTCDALIIATGASAK
                     YLGIPSEDKFRGKGVSACATCDGFFYKGHRVMVVGGGNTAVEEALYLSNLASHVTVVH
                     RRDKFRAEKILQDRLFALEKAGKVRIAWNHTVDEILGDQSGVNGVHIKPTEHGGTIET
                     LDVTGAFIAIGHTPNTGIFASQLDMRDGYLMIKGGCEGGATATTVPGVFAAGDVADHV
                     YRQAITSAGSGCMAALDADKYLDQHHGH"
BASE COUNT          443 a          767 c          836 g          442 t
ORIGIN      
        1 ctggctgccg agggagcgca gatgggcggt aggtagctgg caatcgatca gttggagacc
       61 cgcaacgacc gcccgctcca ccagccgtgc gaatgcgact ttcgacgcgt cccgctccct
      121 gctgaacatc gactcgccga agaacatgcg gccgagcagg acgccataca ggccgccgac
      181 caggacatca tccagccgca cttcgacact gtgtgcgtgg ccctgggcgt gaagctcgca
      241 ataggcagcg tgcatctcgg gcgtgatcca ggtgcccggg gagtgttcgc gacgagccgc
      301 gcaggcgtcg accacgccag cgaagtcgcg gtcgaatgtc actacaaagc cacgatttct
      361 cagagttttg gcaagactgc gcgaacagcg gaattcctcg ggatagagca cttcgcgagg
      421 attcggcgac caccacaaca ccggctggcc cgcggaatac cagggaaaga ttccgcggcg
      481 ataggcggcc aggagccgtg gcaccgacag gtcaccgccc gccgccagca atccttccgg
      541 ctcatcgagc acctggtcga gcggcggaaa ccattcgcgg tcgccgtcgg gtttgagcca
      601 ggtgatggtc ttgttcatgg ccgcatgccc ttagtcgccg cgagcgatcc gtcgggcgtc
      661 gcggtagggg acatgatttt cgatgtcgcg cgcgtaatcg tcgacgtggg cgccttcgcg
      721 ccgcaggtag tctcctatgg cgacccggaa tcttggatcg acgatccagt gcatggacca
      781 ggtgttggcc gggacgaatc cccggctcac cttgtgttcg ccctgggtgc cgggctcgaa
      841 tcgctggata ccccgctcga tgcagaactc gatgccctgg tggtagcagg tttcgaaatg
      901 caggctgtgg tgctgctcgc tggcgcccca gtagcggcca tacagcgcct ccgggctcca
      961 gaaaaacacc gccgcggcca cggtcgcgcc gccggcacgc gcgcgcttga tcatgaaacg
     1021 gtcgccgagt gcgcctggca tttcgcgaaa gaacgcgcgc gagagatagg gctcgtggcc
     1081 atggcgcagg aaagtatcgc ggtgcaggtc gtaagcttcg tcgagtgagg cgcgatcgat
     1141 accggtgcct aacaaagtgt cgaaggtgat gccttcctcg gcgacgcgcc gccgctcgcg
     1201 cttcgctttc ttgcgcttgt cggcggtgaa gctgtcgagg tagtgatcga agtcgcggta
     1261 gccgcggttg tgccagtgga actgcacgtc gcgccgtgcg agccagccgg cttgcgcgaa
     1321 cgcctcgcga tcctgcccgg cgacgaacag gccgtggatc gacgagaaat tccgcgcggt
     1381 ggcgaactcg tgaatggcgg cgatcagcgc ggcgcgggtc ttcgcggcat ccagatccgg
     1441 ccgcacgagc agtcgcgcgc cgctcgccgg cgtgaatggc acaccgagca cgagtttggg
     1501 gtagtactcg agcccgtgct gcgcgtaggc ctgcgcccag gagaaatcga agacgaattc
     1561 gccgaaagaa tgggccttca cataggcagg tacggcggca gcgagtccgc gttcatcgtg
     1621 cagcgtgatc ggcgcgggct cccatcccgt gttcgcgcct acgcagcccg tgcgttcgag
     1681 cgaggccagg aacgcgtgac gcaggaaggg tgagccgccc gcgtcgagtg cgtcccagtc
     1741 ggcgggatca atgtccgtga tgttggtcga ggtacttgtc cgcatcgagc gcagccatgc
     1801 atccggatcc ggcggaggtg atggcctgac gatacacgtg atcggcgacg tcgcccgcgg
     1861 cgaacactcc cggcacggtg gtggcggtgg cgccgccttc gcagccgccc ttgatcatga
     1921 gatagccgtc acgcatgtcg agctggctgg caaagatgcc cgtgttcggc gtgtgtccga
     1981 tggcgatgaa agcgccggtc acgtccaggg tttcgatggt gccgccatgc tcggtgggct
     2041 tgatgtgcac accgttcacg ccggactggt cgcccaggat ttcgtccacg gtgtgattcc
     2101 acgcgatgcg caccttgccc gccttctcga gcgcgaacag gcgatcctgc aggatcttct
     2161 cggcgcggaa cttgtcacgg cggtgcacca cggtcacgtg gctcgcgaga ttggatagat
     2221 agagcgcttc ttccacggcc gtattgccgc cgcccacgac catgacgcga tggcctttgt
     2281 aaaagaatcc gtcgcaggtg gcgcaggcgg aaacaccctt gccgcggaac ttatcttccg
     2341 acgggatgcc cagatacttg gcggaggcgc ccgtggcgat gatcagcgcg tcacaggtgt
     2401 agctgccgta atcgccgttc agaacgaacg ggcgcttcga gaaatcgacc gtatggatgt
     2461 gatcgttgac gatttccgtg ttgaaccg
//
LOCUS       DATDEU010000748         3934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1748440, whole genome shotgun sequence.
ACCESSION   DATDEU010000748 DATDEU010000000
VERSION     DATDEU010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748440"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VIV63_17775"
     CDS             <1..472
                     /locus_tag="VIV63_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEY6126508.1"
                     /translation="RLAGRDVYVREGCYLCHSQMIRTLRFETQRYGDRSVAGEFVYDR
                     PFQWGSKRTGPDLARIGAKYSDAWHRAHLLAPRELVPQSNMPGYPWLSEAKIDGEELQ
                     AKMRTLRMLGDPYTEADISGAADAVLDKTELDALIAYLQGLGVKAPPAVAAVAP"
     gene            469..621
                     /locus_tag="VIV63_17780"
     CDS             469..621
                     /locus_tag="VIV63_17780"
                     /inference="COORDINATES: protein motif:HMM:NF017366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEY6126509.1"
                     /translation="MNEFYGHFIGVLIVVIMLSFIGIWIWAWLPHHEREFSELARLPL
                     DDEDPS"
     gene            618..1526
                     /gene="ccoP"
                     /locus_tag="VIV63_17785"
     CDS             618..1526
                     /gene="ccoP"
                     /locus_tag="VIV63_17785"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEY6126510.1"
                     /translation="MSGFWSVWVMVLACVTAGVGLFLFFWALCMRIPTVEDGTTGHVW
                     AHGALREAVRPLPWWWVLVSIGAFLFGAIYLFLYPGFGNFAGRIGWTSQGEQQRHVAE
                     NDAKLQARLEPLRVLSIEQLASHEEAVGIGHRLYLDNCAACHGTHALGNQAVGAPDLT
                     DADWLYGGDSQTIVASILDGRNGIMPPLGGALGHNGVNYVAAYVVSLSGTQAPADWVA
                     AGKTHFDALCTACHGVDGRGNPALGAPNLIDAAWLSSSNFESVVASVRDGRTGVMPAW
                     RHRLSADEVRLITAWVMAQGMSERVK"
     gene            1646..>3934
                     /locus_tag="VIV63_17790"
     CDS             1646..>3934
                     /locus_tag="VIV63_17790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY6126511.1"
                     /translation="MPGPSSCWHCGEALPASGVIYASVAGVARTMCCQGCRAAAEWID
                     QLGLADYYDLRTAPAQRPAAEGQAAHDAWQNADNARHVVRDLGDGLRESLLLVDGVRC
                     TACVWLIERSLGAMPGVVSVQVNAVAQRARVTWRDGEVTLPRLLQTLARTGYRALPLD
                     ACGLDDLRRRESRDALKRLLVAGFGAMQAMMYATAIYLGAADTLDAPTRELLRWLGLL
                     VATPVVFYSARPFFAGARRSLAARRVGMDVPVALAIAAVYSMSIIEALRGSGEVYFDS
                     VSMFVFFLLAGRYLEMRARHRARDLTDALARLTPPFADRQCEGGVQRVGIHELRVGDV
                     VRVAEGGIVPADGVLLTERCRVDEALLSGESAPVTRHRDDPLIAGSVLEDGPVFVRVE
                     RVGADTALAGIAALVGRAHAERPRLQVAGELAAGRFVARVLALTALTAIVWGFVDPER
                     AFSAAVAVLVISCPCAFALAVPAAFTRALGALARRGVLVVKPDAIQALTECTHAIFDK
                     TGTLTETSLSLSDVQTFDLVSRADALRMAATLARQSRHPVARAIAAADAGVDDAEVRD
                     VKSHAGLGVSATVDGRAMRLGRGDFAVGGSTLAREYDDAVLLADDAGPIAAFRVSERL
                     RTDACAAIDGLRARGVTPLIASGDAHAKVAAVAARLNVDTWRARTLPADKLAWVAELR
                     AQGARVLAVGDGVNDAPILAGADVSIALAEGAELTQASSDIVLTGGRLDAIAAARDIA
                     RQTLAIVRQNQRWALIYNLSAVP"
BASE COUNT          556 a         1308 c         1392 g          678 t
ORIGIN      
        1 gcggctcgcg ggccgcgacg tgtacgtgcg cgaaggctgt tacctgtgcc actcgcagat
       61 gatccgcacg ttgcgtttcg agacgcagcg gtacggcgac aggtccgtcg ccggcgaatt
      121 cgtctatgac cggccgttcc aatggggctc gaaacgcacg ggaccggatc tcgcgcgtat
      181 cggtgccaag tactccgacg cctggcatcg cgcgcacctg ctggcgccgc gtgagctcgt
      241 gccgcagtcg aacatgccgg gttacccgtg gctctcggag gcgaagatcg acggcgaaga
      301 gttgcaggcg aagatgcgca cactgcgaat gctgggtgat ccctacaccg aagccgacat
      361 ctcgggcgcc gcggacgcgg tgctcgacaa gaccgagcta gacgcgctga tcgcctatct
      421 acaggggctg ggtgtcaagg cgccaccggc cgtggcggcg gtcgcgccat gaacgagttt
      481 tacggtcatt tcatcggcgt gctgatcgtg gtgatcatgc tctcgttcat cggtatctgg
      541 atctgggcgt ggctgccgca tcacgagcgg gagttctccg agctggcgcg gctgccgctc
      601 gatgacgagg acccgtcatg agcggcttct ggtccgtgtg ggtgatggtc ctggcctgcg
      661 tgacggcggg cgtcggcctg ttcctgttct tctgggcgct gtgcatgcgc atacccaccg
      721 tcgaggacgg caccaccggc cacgtgtggg cgcacggcgc gctgcgcgaa gcggtccggc
      781 cgctgccatg gtggtgggtg ctcgtgtcca tcggcgcgtt cctgttcggc gccatctatc
      841 tattcctcta cccgggtttc ggcaatttcg ccggccggat cggctggact tcgcagggtg
      901 aacaacagcg ccacgtcgcc gaaaacgacg cgaaactgca ggcgcggctg gagccgctgc
      961 gcgtgctcag catcgaacag ctggcctcac acgaggaggc cgtgggcatc ggccaccggc
     1021 tgtacctgga caactgcgcc gcctgtcacg gaacccatgc gctgggtaat caggcggtgg
     1081 gcgcgcccga tctgacggat gccgactggt tgtacggtgg cgacagccag accatcgtcg
     1141 ccagcatcct cgacggccgc aacggaatca tgccaccgct cggcggcgct ctcggccaca
     1201 acggcgtgaa ctacgttgcc gcctatgtag tcagcctgag cggaacacag gccccggcgg
     1261 attgggtggc ggcgggcaag acgcatttcg acgcgctgtg taccgcctgt cacggcgtcg
     1321 acggtcgcgg caatcccgcg ttgggtgcgc ccaatctcat cgatgccgcc tggctctcga
     1381 gcagcaattt cgaaagtgtg gtcgccagcg tgcgcgacgg gcgcaccggg gtgatgccgg
     1441 cgtggcgtca ccggctttcc gccgacgaag tgcggctgat caccgcgtgg gtcatggccc
     1501 agggaatgag cgagcgcgtg aaatgaaagg cgcaagcgaa ccctggtatc ggcctcccgt
     1561 gatgtggctc ggtgccgccg tgttgctggc gtcgatcgcc ggctgcatcg cgctcattat
     1621 tacggccgcc ggatgaggcg cgcgaatgcc gggtccctct agctgctggc attgcggaga
     1681 agcgctcccc gcgagcggcg tcatatacgc gagcgtcgcg ggcgttgcgc gcaccatgtg
     1741 ctgccagggt tgccgtgccg cggccgaatg gatcgaccaa ctgggtctgg ccgactacta
     1801 cgacttgcga acagctccgg cgcagcggcc ggcggccgag ggccaggcgg cccacgatgc
     1861 ctggcagaac gcggacaacg cccgccacgt cgtgcgcgac ctcggcgacg gattgcgtga
     1921 atcgctgctg ctcgtcgatg gtgtgcgctg cacggcctgc gtgtggctga tcgaacgttc
     1981 gctcggcgcc atgccgggcg tggtcagcgt gcaggtgaat gccgtcgcgc aacgcgcgcg
     2041 tgtcacctgg cgcgacggcg aagtcacctt gccgcggctg ctgcagacgc tggcccgcac
     2101 cggatatcgc gcactgccgc tcgacgcgtg cggcctcgat gacctgcggc gccgtgaatc
     2161 gcgcgacgcg ctcaagcgtt tgctggtcgc gggcttcggc gccatgcagg cgatgatgta
     2221 cgccaccgct atctacctgg gcgccgcgga cacactcgat gcaccgacac gcgagttgct
     2281 gcgctggctc ggcttgctgg tggcgacacc ggtggttttc tattcggcgc ggccgttctt
     2341 cgcgggcgcc cggcgctcgc tcgcggcgcg ccgcgtcggc atggacgtgc cggtggcgct
     2401 ggccatcgcc gcggtctact cgatgagcat catcgaagcg ctgcgcggca gcggcgaggt
     2461 ctacttcgat tcggtgtcga tgttcgtgtt cttcctgctc gcgggccgct acctcgagat
     2521 gcgcgcgcgg catcgcgccc gcgatctcac cgatgcgctg gcgcggctga caccgccgtt
     2581 cgccgaccgc cagtgtgaag gtggcgtaca acgagtcggc atccacgaat tgcgcgtcgg
     2641 cgacgtcgtg cgcgtggccg aaggcggcat cgtgcccgcc gatggggtgc tgctgaccga
     2701 gcgatgccgc gtcgacgaag ccttgctcag cggcgagtcg gcgcccgtga cccggcatcg
     2761 cgacgatccg ctcatcgccg gcagcgtgct ggaagacggg ccagtgttcg tgcgcgtcga
     2821 acgcgtcgga gcggacaccg cgctcgcggg tatcgcggcg ctcgtcggcc gcgcgcatgc
     2881 cgaacgcccg cgattgcagg tggccggcga gttggccgct ggccggttcg tcgcgcgggt
     2941 tctcgccttg acggcgttga ccgccatcgt gtggggcttc gtcgatcccg aacgcgcgtt
     3001 ttccgctgcc gtcgccgtgc tggtgatttc ctgcccctgc gccttcgcgc tcgccgtacc
     3061 ggccgctttc acgcgcgcgc tgggtgcgct ggcgcgccgt ggcgtgctgg tggtgaagcc
     3121 ggatgccatt caggcactca ccgaatgcac ccacgccatc ttcgacaaga ccgggacgtt
     3181 gacggagacc agcctttcgt tgagcgatgt gcagaccttc gatctcgtct cgcgagccga
     3241 cgcattgcgc atggccgcga cgctcgcccg gcagagtcgt catccggtcg cgcgcgcaat
     3301 tgcggccgcg gacgccggcg tcgacgacgc cgaggtgcga gacgtgaagt cgcatgccgg
     3361 attgggcgtg agtgcgacgg tcgacggacg cgcgatgcgc ctcggccgcg gcgacttcgc
     3421 cgtgggtggc agtacgctcg cgcgcgagta cgacgacgcc gtgttgttag ccgatgacgc
     3481 cggtcccatc gcagcctttc gagtgagcga acgcctgcgg acggacgcct gcgccgccat
     3541 cgacggcctg cgcgcccggg gcgtgacgcc cctgatcgcg agcggcgatg cgcacgccaa
     3601 ggtggctgcc gtcgccgcgc ggttgaatgt cgacacctgg cgcgcccgca cgttgcccgc
     3661 cgacaaactc gcctgggttg ccgaactgcg cgcgcagggt gcgcgcgtgc tcgcggtggg
     3721 tgacggcgtc aacgacgcgc cgatactcgc gggcgcggac gtgtcgatcg cgctcgcgga
     3781 gggcgcggag ctcacacagg ccagcagcga catcgtgctc acgggcgggc gtctcgatgc
     3841 gatcgccgcc gcgcgtgaca tcgcacgcca gacgctggcc atcgtgcgcc agaaccagcg
     3901 ctgggcactc atctataacc tgtccgccgt tcct
//
LOCUS       DATDEU010000749         2922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_143775, whole genome shotgun sequence.
ACCESSION   DATDEU010000749 DATDEU010000000
VERSION     DATDEU010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143775"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2621)
                     /gene="secA"
                     /locus_tag="VIV63_17795"
     CDS             complement(<1..2621)
                     /gene="secA"
                     /locus_tag="VIV63_17795"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY6126512.1"
                     /translation="MLNLLTRLFGSRNDRIVKGYETTVRKAAAFETALQALSDEALGA
                     KTVEFRKRLADGTKLIDLAPEGFAVVREAARRTLKMRHFDVQLIGGLTLNDGRIAEMR
                     TGEGKTLMSTLAAYLNALTGDGVHLVTVNEYLAQRDADWMGPIYRFLGLTVGVIKNQQ
                     DPGEKRAAYRCDITYGTNNEFGFDYLRDNLAFSLDDRVQRGLAFAIVDEVDSILIDEA
                     RTPLIISGPAEENEDLYVKVNTLVPRLKKQLEEDGPGDFSVDEKQKQTHLTEAGHETV
                     EGLMAEAGLLREGESLYDPQNIRLMHHLNAALRANAIYKRDVEYIVRGGEVIIVDEFT
                     GRTMPGRRWSDGLHQAVEAKEGVKVRAENQTVASITFQNYFRLYKKLSGMTGTADTEA
                     PEFMQIYGLEVVVIPTHRPMIRKDNADFVYLTQGDKFKAIIEDIEDCVSREQPVLVGT
                     TSIETSELLSGVLKENGIPHEVLNAKQHEREAHIVENAGMPGKVTIATNMAGRGTDIK
                     LGGNNEAELEQAAAAAGEAGLDEVTAARIRSDWQARHDKVVAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSMEDNLMRIFGDPNRTKKWLAMAGMKEGEVIESGLL
                     TRQIEKAQRKVEAHNFDVRKQLLTFDDVANDQRKVIYQQRTELMGATDLADAIRGIRG
                     EVVSGILDQHLPANVSHEEWNLDGLAASIAKSFATAVDPAQWLKDQPELEESTLRGRI
                     VGAIDAMYDAKVERIGVPIMRHVEKDIMLKVLDQQWREHLGAMDYLRQGIHLRGYAQK
                     DYRYEYKREAFELFAAMLERIKFDTVTILATLDVQVKSPEQIEREEEARRARLNRALQ
                     AQHAEAASLSQLGAVAEP"
BASE COUNT          430 a          893 c          979 g          620 t
ORIGIN      
        1 ggttccgcca cggctccgag ctgcgacagt gatgcagctt cggcgtgctg cgcctgcagc
       61 gcgcgattga gacgcgcgcg gcgcgcctct tcctcgcgct cgatctgttc ggggctcttc
      121 acctgcacat cgagcgtcgc caggatggtg acggtgtcga acttgatgcg ctcgagcatg
      181 gccgcgaaca gctcgaaggc ctcgcgcttg tattcgtagc ggtagtcctt ctgcgcataa
      241 ccgcgcagat ggatgccctg gcgcaggtag tccatggcgc cgagatgctc gcgccattgc
      301 tggtcgagca ccttgagcat gatgtctttt tcgacgtgcc gcatgatggg cacgccgatg
      361 cgctccacct tggcgtcgta catcgcgtcg atggcgccga cgatgcggcc gcgcagcgtc
      421 gattcctcga gctcgggctg atccttgagc cactgcgccg gatcgaccgc ggtcgcgaag
      481 ctcttggcga tggaggccgc caggccgtcg agattccact cttcgtggct gacgttggcc
      541 ggcaggtgct ggtcgaggat gccgctaact acttcgccgc ggatgccgcg gatggcgtcg
      601 gccaggtcgg tggcgcccat gagctcggtg cgctgctggt agatgacctt gcgctggtcg
      661 ttggccacgt cgtcgaacgt cagcagctgc ttgcgcacgt cgaagttgtg cgcctcgacc
      721 ttgcgctgcg ccttctcgat ctggcgcgtg agcagtccgc tctcgatgac ctcgccttcc
      781 ttcatgccgg ccatggccag ccacttcttc gtgcgattcg gatcgccgaa gatgcgcatg
      841 aggttgtctt ccatcgacag atagaagcgg ctcgaacccg gatcgccctg gcggcccgaa
      901 cggccgcgca gctggttgtc gatgcggcgc gactcgtggc gttcagtgcc gatgatgtgc
      961 agaccgccgg cggcgaccac cttgtcgtgg cgcgcctgcc agtcggagcg gatgcgtgcg
     1021 gcggtgactt cgtcgagccc cgcttcaccc gcggcggccg cggcctgctc gagctcggct
     1081 tcgttgttgc cgccgagttt gatgtcggta ccgcgaccgg ccatgttcgt ggcgatggtc
     1141 actttgcccg gcatgccggc gttctcgacg atgtgcgcct cacgctcgtg ctgcttggcg
     1201 ttcagcactt cgtgcgggat gccgttttcc ttgagcacgc cggagagtaa ttcggacgtc
     1261 tcgatggacg tggtgcccac gagcacgggc tgctcgcgtg acacgcagtc ttcgatgtcc
     1321 tcgatgatgg ctttgaactt gtcgccctgc gtgaggtaca cgaagtccgc gttgtccttg
     1381 cggatcatcg gacggtgcgt ggggatcacg acgacttcga ggccgtagat ctgcatgaac
     1441 tccggcgctt cggtgtcggc cgtgccggtc atgccggaga gtttcttgta gaggcggaag
     1501 tagttctgga acgtgatcga cgcgacggtc tggttctccg cgcgcacctt cacgccttcc
     1561 ttcgcttcca cggcctggtg caggccgtcg gaccagcggc ggcccggcat cgtgcggccg
     1621 gtgaactcat cgacgatgat gacttcgccg ccgcgcacga tgtattccac gtcgcgcttg
     1681 tagatcgcgt tcgcgcgaag cgccgcgttc aggtgatgca tgaggcggat gttctgcggg
     1741 tcgtagaggc tctcgccttc gcgcagcagc ccggcttcgg ccatgaggcc ttcgacggtt
     1801 tcgtggccgg cttcggtgag atgcgtctgc ttctgttttt cgtcgaccga gaaatcgccg
     1861 gggccgtcct cttcgagctg cttcttgagc cgcggcacca gcgtgttgac cttcacgtac
     1921 aggtcttcgt tttcttcggc cgggccggag atgatcagcg gcgtgcgcgc ttcgtcgatg
     1981 aggatggagt cgacttcgtc gacgatggcg aacgcgaggc cgcgctgcac gcgatcgtcg
     2041 agactgaacg cgaggttgtc gcgcaggtag tcgaacccga attcgttgtt ggtgccgtag
     2101 gtgatgtcgc agcgatacgc ggcgcgcttc tcgccgggat cctgctggtt cttgatgacg
     2161 ccgaccgtga ggccgaggaa gcggtagatg ggacccatcc agtccgcgtc gcgctgcgcc
     2221 aggtattcgt tgacggtgac gaggtgcacg ccgtcgccgg tgagcgcgtt gaggtacgcg
     2281 gcaagcgtcg acatcaacgt cttgccttcg ccggtgcgca tctcggcgat gcggccgtcg
     2341 ttcagcgtga ggccgccgat gagctgcacg tcgaagtggc gcatcttcag tgtgcggcgc
     2401 gcggcttcgc ggaccaccgc gaacccttcg ggcgcgaggt cgatgagctt ggtgccgtcg
     2461 gcgagacgtt tgcggaattc gacggtcttg gccccgaggg cctcgtcgct caatgcctgc
     2521 aatgcggtct cgaacgccgc cgccttgcgc accgtcgttt catagccttt gacgatgcga
     2581 tcgttgcgac tgccaaagag ccgcgtcaga agattgagca cttaagaatc cttgcgagaa
     2641 acttcaagcc agacagccac ttagaatagt gcgccgtgaa gccgggccgg tattctacgg
     2701 atagaccctc atccacggta ctggtttcca ccctgagcca gacgccgagg tattgggctg
     2761 gcgcgagggg aaaaaaagcc ccgcgtcgcg gggctttggg atcaacaggg caggtccggc
     2821 cccttttgct aggaaatggg gagtgtcccc atttcctgga ggcggtcagc gaccgatgaa
     2881 ggtcagcggg tcgatctgcc ggccttcctt gagcacttcg aa
//
LOCUS       DATDEU010000750        11512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1603100, whole genome shotgun sequence.
ACCESSION   DATDEU010000750 DATDEU010000000
VERSION     DATDEU010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11512
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603100"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="VIV63_17800"
     CDS             <1..1625
                     /locus_tag="VIV63_17800"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY6126513.1"
                     /translation="LHDEPGRAAVKSFWGSPAIASKQGPKAVEMFDRIHDGTIKAVWI
                     MATNPVVSLPDADKVKAALQKCEFVAVSDVMGNTDTTALAHVLLPALAWGEKDGTVTN
                     SDRTISRQRPFLPPPGEARADWRVICEVARAMGFEGFDFISPHEIFREHARLTAHHND
                     GRRALNLGAWENITAVEYKNWEPAAWPMAETRDSARGPMFGDGRFLHADGKARFIALA
                     PRLPENAPTQEHPLVLNTGRVRDHWHTMTRTGKSARLSAHIAEPYAEINTADALRFKV
                     RAGELATLRSHYGSMVVRVRTGSEIPPGMVFAPIHWNRAFASDARVGALTNPVVDPIS
                     GEPELKHTPVSIEHFPATWYAVMLTRSERPPPVTQWWTKVQGEKFMRYELAGNHSRDW
                     SIEARRLLGVPADDVLSDVDWIEYRDSARRVYRAAWFVHDRLEGCIYFDRLPALPERA
                     WLASLFTQAKLDAPARASVMAGRALDGCDQGALVCSCFGVGANPIAACARELGAAATP
                     AEIGKRLKCGTNCGSCVAEIQVIIGDVAKKTA"
     gene            1665..2498
                     /locus_tag="VIV63_17805"
     CDS             1665..2498
                     /locus_tag="VIV63_17805"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6126514.1"
                     /translation="MKLQAFVLTLVCCTASAAQPQKLFLWPGDAPGANPSGGEETVRI
                     SDQGDHVVSNVHRPSITVYLPEKIHTGTAVIVVPGGGHRELWTDHEGHNVARFLNERG
                     IAAFVLQYRLARAPNSSYTIDGDALGDLKRAIRTVRSRADWKADTLRVGAMGFSAGGQ
                     LVALAATRGDAGDASAVDPVDKVSSKLDVAALVYPGTWPDLAIDANTPPMWLLCGSDD
                     RPEVIAGLTNLYLKLRAAKVPAEMHLYDGVPHGFGLRTTLKGPVANWPQQFVDWLSIA
                     R"
     gene            complement(2512..3399)
                     /locus_tag="VIV63_17810"
     CDS             complement(2512..3399)
                     /locus_tag="VIV63_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126515.1"
                     /translation="MIPVKRPATAIPAPWAIKAEAETAAAIAAYKAALKKWRTLKGKK
                     KKPLDFTFKYKVYGDDLVRAAINDLYKFKCAYCETHYGVSQPVAVEHYRPKGEVIEGK
                     ARVKPGYYWLAATADNLLPSCTDCNSPRRQPEPDGTERVRGKGNHFPLAKGSRRAKTP
                     GGEKREVPLLLHPEKDEPEKHLEFLVASDRLGVVRAALINGIPSPKGEASIEVCALDR
                     PRLVMDRRSEAMRLVSHLNNTRDWLRLHRSNPANPDFKKSFDRNIAELREMIGQGQKY
                     LAMQKAILRAEFPEHANLL"
     gene            complement(3396..5525)
                     /locus_tag="VIV63_17815"
     CDS             complement(3396..5525)
                     /locus_tag="VIV63_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6126516.1"
                     /translation="MILQTQSFFDADEKSRRQQKFEFPLFPTPLFNTAMHALIEYSSG
                     KCAYCESPIEHQGNATLERFRPKGGALGLEGDFSTEHYWWLAFDWRNIYPACVYCNKV
                     KGSKFPVVGQRCRPKTPYEGLGGESRLLVDPALDEPADHLRFTGDGHVRAASQIGEAT
                     IATLELNRTELVTARQVVARKTNSLVGSSAVRAALRQWPHDWQAPLVVASKTEGSNSA
                     LTALAALVARAQPFAAVARAIVLEVIAQKSSKSAAPPSKGSPAMAAPAAEPAHVPDQP
                     KLKPIKQGKSPLRSQTITRIEMKNFRGIADLDLEIDFSTGLGAPWTVLLGENGAGKSS
                     ILQALALTLLNSDSAGPAVRPKQVLRKGASSGFVRVWFHATDVPRELHFRRGAHRFVR
                     KGPKYNGTLLGYGATRLLPRQRMKQDDSTVRLQNMFDPFHPLLDANHWLGKLEKRSFD
                     FVARALRDVMDLPRNATLRRVRSSKNAGVRLKQYGADLTLEDLSDGYQSVLGLTCDII
                     ASLRALQPGALDASEAVVIIDELGAHLHPRWRMRIVDSLRRAFKRVQFIGSTHDPLCL
                     RGLENGEAVVLRRTARGRVFPVPDLPPVKGLRVDQLLTSEFFGLDSTLDPAIESQYRE
                     LYRLLAQRDPSDAVNERIAELREALAPYEMPGATRRERRLLQAIDKELAQLDVTPNVA
                     DREQIRAENDEIIQQLLNAPVANKAPA"
     gene            5789..7753
                     /locus_tag="VIV63_17820"
     CDS             5789..7753
                     /locus_tag="VIV63_17820"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEY6126517.1"
                     /translation="MKFRVNVAALGLFLLVPPVFSAGIPATVRSPDQQIVLRLAQNDS
                     GHLTYSIERKGEPVFAASALRVQLAEGDVSSVDVRQVWPRSINQTRKLVATKAAEARD
                     QFNELTVDVAPRSRAVRAMKFVFRVYDDGVAFRFVVPADAGVSTLNVKSEDTEFTFGA
                     DYDCTGLNIGRVDSSHEGEFDPVQASRIREHHNYDLPLVCRTAKNAFAIGEADLRDYG
                     GLYLTGRGDGKPGVLSRISRRLDDRSLIARAAVGANGTGSPWRVIMLGDELGNLIESN
                     LIGNLNPDPAFDTSWIKPGKTAWDWWSGPYLPPPDKGGTDMPTIKRYVDFASSAGFEY
                     MMIDEGWCLNSGTGGRAPDNADVTQAKPEIDMPALVAYAAKKKVKLWLWVQWSLLDRQ
                     MDAALAQYEKWGIAGIKVDFMDRNDQQMVDYYHRLMAKAAGHKLLVDMHGAYPPAGLN
                     RTYPNYLTQEGIMGAEYNKWSRRVTATHNVSLAYTRGLLGPMDYTPGGFRNATPATFE
                     VVNSPPQVQTTRGHGLGMFVVYESPFQMVADSPDIYQNAAGFDFVKMVPVVWDETRFL
                     QGDIDDYVVVARRKGRDWYIGAMGNEKAHDLKIPLSFLGAGKFKAKSWEDGATPTTLK
                     ETEREVGATDGLEIKLAPSGGAAVRVSPLK"
     gene            7764..8318
                     /locus_tag="VIV63_17825"
     CDS             7764..8318
                     /locus_tag="VIV63_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126518.1"
                     /translation="MPTTNGHRRRIGLSLALSVISLTFGNDCAAAESAREEEKETIVV
                     GANHAHSAEIDTVTLIGKVPDNGKKIGVTAKFSETEPKLDRLSILTPEGKTVLVPRSY
                     YEKIRLPREESLSLGYIMAGDSNRVGGIIVFLDFGNLRRRADLNCTNDTGDPVFESFT
                     LSYDLQKRTFSGVFRDYCGEPVSK"
     gene            complement(8408..9265)
                     /locus_tag="VIV63_17830"
     CDS             complement(8408..9265)
                     /locus_tag="VIV63_17830"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY6126519.1"
                     /translation="MTPPAQIDWNLKGKRIAVPEMRELEVFSGLLERRGAEVLRCPLV
                     TIYDTPHSAQVLAFAVKLADGGFDDFVLITGEGLTRILSCIDKYEPALRPRFIEGMSK
                     LRTITRGPKPARALRVLGLKPGIEATEPTTDGVIKSLSAHPLEGRRMAVQLYGNDPNL
                     TLMRFLRERLAQVSTVAPYVYGNAADDVTVHALLERMAAGEVDAIAFTSKLQIERLVT
                     QHPAALVRRALTRTKIAAVGPIVAEAIRAAGFEVASSPEHSWFMKPLVVALSEQLGAK
                     KDKDLSGLA"
     gene            complement(9369..10583)
                     /locus_tag="VIV63_17835"
     CDS             complement(9369..10583)
                     /locus_tag="VIV63_17835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEY6126520.1"
                     /translation="MINREFLKAGHLPTLLSAFFYFDMSFMVWVLLGALAVQMSAELS
                     LNAAQKGFMVALPVLAGALLRVVFGILVDRLKPKRAGIIGQVIVIAGLATAWLVGIKS
                     YQGTLLLGLVLGVAGASFAVALPLASRWYPPQYQGTALGIAGAGNSGTVFASLFAPGL
                     AVLVGWNNVLGLAVLPLALAFVVYLFAAKDSPTTPPPKKLHEYLAVFRMGDAWWFMFF
                     YAVTFGGFVGLASSLAIYFNTDYGLGAVQAGYFTAACVFCGSLVRPIGGALADRIGGI
                     KTLSIMYGAAALALFFVSFHPQSVFGALALFAGAMFAFGMGNGAVFQLVPQRFRHEIG
                     VMTGLVGMTGGVGGFFLASSLGLSKQLTGTYQAGLLLFASLAVLALVGLTIIKHRWRT
                     TWGAVGVAGARI"
     gene            10621..>11512
                     /locus_tag="VIV63_17840"
     CDS             10621..>11512
                     /locus_tag="VIV63_17840"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY6126521.1"
                     /translation="MQGADHGTADGLQVGVGHSTHQGTRADNQDYAACYVGPARNQLT
                     IGLLAALADGMGGAKGGRVAAELAVRGFIDGCLGQPSTIGLSRIGARAADAVNRWIHT
                     LGRSDPGLNGMACTLSALLLCGRGTHLVHVGDSRVYRMRDNELTLLTTDHTLGAPGTT
                     HALTRAVGAQDSLRVDHFKETAHLHDRYLLCSDGVHGALSQKQIHALLARRAAPDETA
                     RELVAQAAASTDSDNCTAIVLDILALPSTQFADMELAIGEQPLRAAPSSGAVIDDYEL
                     GEMLADGQYMRVFRARDHRAS"
BASE COUNT         2062 a         3551 c         3834 g         2065 t
ORIGIN      
        1 atctgcacga cgaacccggt cgcgccgcgg tgaagagttt ctggggaagt ccggcgatcg
       61 ccagcaagca aggtccgaag gccgtcgaga tgttcgaccg catccatgac ggcacgatca
      121 aggccgtgtg gatcatggcc accaacccgg tagtgagtct gccggatgcc gacaaggtga
      181 aggcggcgct gcagaagtgc gagttcgtcg cggtgtccga tgtcatgggg aacaccgaca
      241 ccacggcgct ggcgcacgtg ctgctgccgg cgctcgcctg gggcgagaaa gacggcaccg
      301 tgaccaactc ggatcggacc atctcgcggc agcggccatt cctgccgccg ccgggcgaag
      361 cgcgcgcgga ctggcgcgtg atctgcgaag tggcccgcgc catgggtttc gaaggattcg
      421 acttcatctc gccgcatgaa atcttccgcg aacatgcgcg gctcacggcg catcacaacg
      481 atggccggcg cgcgctgaac ctgggtgcgt gggagaacat caccgccgtc gaatacaaga
      541 actgggagcc cgcggcctgg cccatggccg agacgcgcga ctccgcgcgc gggccgatgt
      601 ttggcgacgg gcggttcctg catgcggatg gcaaggcacg tttcatcgcg ctcgcgccgc
      661 gcctgccgga gaacgcaccg acgcaggaac atccgctggt gctcaatacg ggccgggtgc
      721 gcgaccactg gcatacgatg acgcgcacgg gaaagtccgc gcggctctcg gcgcatatcg
      781 cggaacctta cgccgagata aacaccgcgg acgcactgcg cttcaaggtt cgcgcgggcg
      841 agctcgcaac attgcggtcc cattacggct cgatggtcgt gcgcgtgcgt acgggatcgg
      901 agattccacc gggcatggtg ttcgcgccta tccactggaa ccgggcgttc gcatccgatg
      961 cgcgcgtggg cgccctcacc aatccggtgg tcgatcccat ttccggcgag cccgagctca
     1021 agcacacgcc cgtctcgatc gaacatttcc ccgccacctg gtacgccgtc atgctcacgc
     1081 gcagcgagcg gccgccgccg gtgacgcagt ggtggacgaa agtgcagggc gagaagttca
     1141 tgcgttacga gctcgccggc aatcattcgc gcgactggtc gatcgaggcg cgccgcctgc
     1201 tcggcgtgcc ggcggacgat gtgctctccg acgtcgactg gatcgaatat cgcgactcgg
     1261 cgcggcgcgt gtatcgcgct gcctggttcg tgcacgaccg gctcgagggc tgcatctact
     1321 tcgaccggct gccggcgctg ccggaacgcg cgtggctggc cagcctgttc acgcaggcga
     1381 agctcgacgc gccggcgcgc gcgagcgtca tggcgggccg tgcgctcgac ggttgcgatc
     1441 agggcgcctt ggtctgctcc tgcttcggcg tcggcgccaa ccccatcgcg gcctgcgcgc
     1501 gcgagctcgg cgccgcagcg acgcccgccg agatcggcaa gcgattgaag tgcggcacca
     1561 actgcggctc ctgtgtggcg gagatccagg tgatcatcgg cgacgtcgcg aagaagaccg
     1621 cctgaactct ggtagtgttc cgggcttcca cgagaggggg tcccgtgaag ctgcaagcct
     1681 ttgtactgac actcgtctgt tgcacggcca gcgccgcgca gccgcagaag ctgttcctgt
     1741 ggcccggcga cgctccgggc gccaaccctt cgggtggcga agaaaccgtg cgcatcagcg
     1801 accagggcga ccacgtggtg tcgaacgtgc accgcccgtc gatcacggtc tatctacctg
     1861 agaagatcca cacaggcaca gcggtcatcg tggtgccggg cggcggtcat cgcgagctct
     1921 ggaccgacca cgaaggtcac aacgtggcgc ggttcctcaa cgaacgcggc atcgcggcct
     1981 tcgtgctgca gtaccggctg gcgagggcgc ccaattccag ttacaccatc gacggtgatg
     2041 cgctgggcga tctgaagcgc gccatccgca cagtgcgctc gcgtgccgat tggaaagcgg
     2101 acacgctccg ggtcggcgcc atggggttct ccgcgggcgg ccagctcgtg gcgctcgccg
     2161 ccacgcgtgg cgatgcgggc gatgcgtcgg cggtcgatcc cgtcgataag gtttcgtcga
     2221 agctcgatgt tgccgcgctg gtatatccgg gaacctggcc ggatctcgcg atcgacgcca
     2281 atacaccgcc catgtggctg ctgtgcggca gcgacgaccg gcccgaggtc atcgccggcc
     2341 tcaccaacct ctatctgaag ctgcgagccg ccaaggtgcc ggccgagatg cacctgtacg
     2401 acggcgtgcc gcatggtttc gggctgcgta cgacgctcaa aggtccggtg gcgaactggc
     2461 cgcagcagtt cgtcgactgg ctgtcgatcg cgcgctagct accgcgatgc gtcaaagcag
     2521 atttgcgtgc tcgggaaact ctgcgcgcag gatggccttc tgcatggcga gatacttctg
     2581 accctgaccg atcatctcac gcagttccgc gatattgcga tcgaaagatt tcttgaagtc
     2641 tggattcgcc ggattgcttc ggtgaagccg tagccagtcc cgggtattgt tcaggtgtga
     2701 caccaggcgc atcgcctcgg atcgccggtc catcacgagt cgcggcctgt ccagcgcgca
     2761 tacctcgatc gaagcttcgc ctttcgggct tggaatcccg ttgatcagcg ccgcgcgaac
     2821 cacgccgagc cggtcgctgg caaccagaaa ttcgaggtgt ttttctggct cgtccttttc
     2881 gggatgcagc agcagcggga cttcacgctt ctcgcctccc ggcgtcttcg cccgacgcga
     2941 gcccttggcg agcggaaagt ggttcccctt gccacgcacg cgctcggtgc cgtctggttc
     3001 cggctggcgg cgcggtgagt tgcagtccgt acaactcggc aaaagattgt cggcggtagc
     3061 agcaagccag tagtaacccg gtttgacgcg tgcctttcct tcgatgactt cgccctttgg
     3121 acgatagtgt tcgaccgcca ccggctgcga gacaccatag tgtgtttcgc agtacgcgca
     3181 tttgaacttg tagaggtcgt tgatcgcggc gcggaccagg tcgtcgccat acaccttgta
     3241 cttgaacgtg aaatcgagtg gtttcttctt tttgcctttc agcgtcctcc atttcttgag
     3301 tgctgccttg taggcagcaa tggccgcggc ggtttccgcc tcagccttga tggcccacgg
     3361 cgcgggaatc gcagtggcgg gtcgcttgac ggggatcatg caggcgcctt gttagccacg
     3421 ggtgcgttca ggagctgttg aatgatttca tcgttctcgg cgcgtatctg ttcgcgatcg
     3481 gcgacgttgg gagtgacgtc gagttgcgcc agctccttgt cgatggcctg caacagccgc
     3541 cgttcgcgtc gcgtggcccc cggcatctcg tagggcgcca gcgcttcgcg cagttcggct
     3601 atccgttcgt tgacggcgtc gctggggtca cgctgggcga gcaggcgata caactcgcga
     3661 tactgggact cgatggccgg atcgagcgtc gagtcgagac cgaagaattc ggatgtgagc
     3721 aactgatcga cgcgcagacc cttgaccggc ggcagatcgg gcacgggaaa cacgcggccg
     3781 cgcgcggtac ggcgcagcac cacggcctcg ccgttctcga gtccgcgcag gcagagcgga
     3841 tcgtgcgtgc tgccgatgaa ctgcacgcgt ttgaatgcac gcctcaggct gtcgacgata
     3901 cgcatgcgcc agcgcggatg caggtgcgcg ccgagttcat cgatgatgac tacggcttcg
     3961 ctggcatcga gcgcgcccgg ttgcaacgcc cgcagcgagg cgatgatgtc gcaggtgagg
     4021 ccgagcacgg actggtaacc gtcgctcagg tcttcgagcg tgaggtccgc gccgtattgc
     4081 ttcaggcgca ctcccgcgtt cttgctcgaa cgaacgcgtc gaagcgtggc attgcgaggc
     4141 aggtccatga cgtcacgcag cgcacgcgcg acgaaatcga aggagcgttt ctcgagtttg
     4201 ccgagccagt ggttggcatc gagcagcgga tgaaacgggt cgaacatgtt ttgcagtcgg
     4261 accgtcgagt cgtcctgctt cattcgttgg cgcggtagca ggcgcgttgc accgtagccc
     4321 agcagcgtgc cgttgtactt cggccccttg cgaacgaagc gatgcgcgcc gcgtcgaaaa
     4381 tgcagttcgc gcgggacgtc ggtcgcgtgg aaccacacgc gcacgaatcc gctcgatgcg
     4441 cccttgcgca atacctgttt ggggcgcacg gcgggcccgg cggaatcgct gttcagcagc
     4501 gtcaacgcca gcgcctgcag gatcgacgac tttccggcgc cgttttcacc taacaagact
     4561 gtccacggcg cgccgaggcc cgtcgagaag tcgatctcca ggtcgagatc ggcgatgcca
     4621 cgaaagttct tcatctcgat gcgcgtgatg gtctgcgagc gaaggggcga cttgccttgc
     4681 ttgatcggct tgagttttgg ttgatcgggc acatgagctg gttcagcggc aggcgccgcc
     4741 atcgcaggtg aacccttcga aggcggcgcg gccgatttcg agcttttttg ggcaatgacc
     4801 tccagaacga tcgcccgcgc cacggcggcg aatggctgcg cgcgggcgac gagcgcggcc
     4861 agcgccgtca atgcgctatt cgacccttcg gtcttgctgg cgaccacgag aggggcctgc
     4921 cagtcgtgcg gccattgacg aagtgcggcg cggacggcgc ttgagccgac caacgagttt
     4981 gttttgcgtg caaccacctg ccgggcggtc acgagttcag tccgattgag ttcgagcgtg
     5041 gcgatcgtcg cttcaccgat ctgcgatgcc gcgcgcacat gtccgtcgcc ggtgaaccgc
     5101 aaatggtcgg cgggctcgtc gagcgccggg tccaccaata gtcgcgattc gccgccgagc
     5161 ccttcatacg gagtcttcgg ccgacatcgc tggccaacga cggggaattt cgagcccttc
     5221 accttgttgc agtagacaca ggctgggtag atgtttcgcc aatcgaaggc gagccaccag
     5281 taatgctctg tcgagaaatc accttcgagg ccgagcgcgc cgcccttcgg ccgaaagcgc
     5341 tcgagcgtcg cattgccctg atgctcgatc gggctttcgc agtaggcgca ttttccgctc
     5401 gaatattcga tgagagcgtg catcgccgtg ttgaacaggg gtgtgggaaa cagcgggaac
     5461 tcgaactttt gctggcgccg ggatttttcg tcggcatcga agaaactttg ggtctgcaga
     5521 atcattcgct gcacgggtgg cagggcgaag atgtcgggcg ctggagttcg aggtggcagt
     5581 cggatcatgg gattgtcact tcctgtggtt gactcagctg cattgcagca ctccagagaa
     5641 tcatcgacac tccgtcgccg tcgagtatca atcttttgca ggtggaccgt cccacccgca
     5701 cctgaggtac ccaggttcgt ttcgtgaaat aagtccgcac gttttgggta ttgttcgccg
     5761 cgctcgtcga ttgtccaaag ggggattcat gaaatttcgg gtcaatgtgg ccgcgctcgg
     5821 cctgttcctg ctcgtgccgc ccgtgttttc ggcgggcata cccgccaccg tccgatcccc
     5881 cgaccagcag atcgttctgc ggcttgcgca gaacgattcc ggtcatctca cgtattcaat
     5941 cgagcgcaag ggtgaaccgg tgtttgccgc ttcggcgctt cgagtgcagc tcgccgaagg
     6001 cgacgtctcg agtgtcgacg tgcgccaggt ctggccgcga agcatcaacc agactcgcaa
     6061 gctcgtcgcg accaaggccg cggaagcgcg cgatcagttc aatgagctca cggtcgatgt
     6121 ggcgccacgc tcgcgtgccg tgcgcgcgat gaagttcgtc ttccgcgtgt acgacgatgg
     6181 cgtggcgttt cgctttgtcg tgcccgccga cgcgggtgtg tcgacactca acgtgaagtc
     6241 cgaagacacg gagttcacct tcggcgccga ctacgactgc acgggtctca acatcggccg
     6301 cgtggactcg agccacgaag gcgagttcga tccggtccag gcgagccgca ttcgcgaaca
     6361 tcacaactac gacctgccgc tggtgtgccg cacggcgaag aatgcgttcg ccatcggcga
     6421 agcggatctg cgcgattacg gcgggctcta tctcaccggg cgcggcgacg gcaagcctgg
     6481 cgtgctgtcg cggatttcgc gccggctcga cgatcgttcg ctgattgcgc gtgcggcggt
     6541 cggagcaaac ggcacgggct cgccgtggcg cgtgatcatg ctgggcgacg agctgggcaa
     6601 tctcatcgag tccaatctca tcggcaacct caatccggat ccggcattcg acacgtcgtg
     6661 gatcaagccc ggcaagacgg cctgggactg gtggtcgggt ccctatctac cgccaccgga
     6721 caaaggcggc accgacatgc cgacgatcaa gcggtacgtc gatttcgcgt cgagcgcggg
     6781 cttcgagtac atgatgatcg acgagggctg gtgcctgaac tcgggtacgg gcgggcgggc
     6841 gccagacaac gcggacgtga cgcaggccaa acccgaaatc gacatgccgg cgctggtggc
     6901 ctacgcggcg aagaagaaag tgaagttgtg gctgtgggtg cagtggtcgc tgctcgaccg
     6961 gcagatggac gccgcgctcg cgcagtacga gaagtggggc atcgcgggca tcaaggtcga
     7021 cttcatggac cgcaacgacc agcagatggt cgactattac caccggctca tggcgaaggc
     7081 cgcggggcac aagctgctgg tcgacatgca cggtgcgtat ccgccggccg ggctcaatcg
     7141 tacctatccc aactacctca cccaggaagg catcatgggt gcggagtaca acaagtggag
     7201 ccggcgcgtc actgccacgc acaacgtgag cctcgcgtac acacgcgggt tgttaggacc
     7261 catggattac acgccgggcg ggtttcgcaa tgcgacgccg gcaacgttcg aagtggtgaa
     7321 ttcgccgccg caggtgcaga cgactcgtgg gcacgggctc gggatgttcg tggtgtatga
     7381 gagtccgttc cagatggtgg ccgacagccc ggatatttat cagaacgcgg ccgggttcga
     7441 tttcgtgaag atggtgccgg tggtgtggga cgagacgcgt ttcctccaag gcgacatcga
     7501 cgactacgtg gttgttgcgc gacgcaaggg ccgggactgg tacatcggtg cgatgggcaa
     7561 tgagaaggcg cacgacctga agattccgct gagctttctg ggcgcaggca agttcaaggc
     7621 gaagtcgtgg gaagacggcg cgacgccgac gacactgaag gaaaccgagc gtgaagtggg
     7681 cgcaacggat ggactcgaaa tcaagctggc gccgtcgggt ggtgctgccg tcagagtgtc
     7741 gccattgaaa taaggaagac gctgtgccca ccacgaacgg tcatcggcgg aggatcgggt
     7801 tgtcgctggc cctttccgtg atttcgctga ctttcggcaa tgactgtgca gcggcggagt
     7861 cggccaggga agaggaaaaa gaaacgatcg tcgtcggcgc gaatcacgcc cattcggcag
     7921 aaattgacac ggtcacgctg atcggcaaag tgcccgacaa cggcaagaag attggtgtga
     7981 cggcgaagtt ttccgagacc gagcccaagc tcgatcggct ttcgatactg accccggaag
     8041 ggaagaccgt cctggtgccg cgctcttact acgaaaagat tcgcctgccc cgtgaggagt
     8101 cgctgtcgct gggatacatc atggccgggg actcgaatcg cgtgggcggg atcatagtgt
     8161 tcctggattt cggaaacttg cgccgccgcg cggacctgaa ctgcacgaac gacacgggcg
     8221 atcctgtgtt cgaatcattc acattgtctt atgacttgca gaagcgaact ttttccggcg
     8281 tgttcaggga ttattgcggc gagccagtct cgaagtgaga aatcgaggtc cggccccttt
     8341 tgctagctaa acgagtgggg acagacttaa gtcagtcccc acacatccat gtccccattt
     8401 tccccgttta cgccaggcct gacaggtcct tgtccttctt cgcgcctaac tgctcgctga
     8461 gcgcgacgac cagcggcttc atgaaccagg agtgctcggg gctcgacgcc acttcgaagc
     8521 cggcggcgcg aatggcctcg gcgacgatgg ggccgacggc ggcgatcttg gttcgcgtca
     8581 gggcacggcg cacgagcgcg gcggggtgct gcgtcacgag ccgctcgatc tgcagcttgc
     8641 tggtgaacgc gatggcatcc acttcacccg cggccatgcg ttcgagcagc gcgtgcacgg
     8701 tgacgtcatc ggcggcgttt ccatagacat acggtgcgac ggtactgacc tgcgcaagcc
     8761 gctcgcgcag gaagcgcatg agcgtgaggt tggggtcgtt gccgtagagc tgcacggcca
     8821 tgcgacggcc ttcgagcgga tgggcggaca gggacttgat cacgccatcg gtggtgggct
     8881 cggtggcttc gatgccgggc ttgagcccca gcacgcgcag cgcgcgggcc ggtttcgggc
     8941 cgcgcgtgat cgtgcgtagt ttgctcatgc cctcgatgaa gcgagggcgc aaagcgggtt
     9001 cgtatttgtc gatgcagctc aggatgcgcg tgagcccttc gccggtgatc agcacgaaat
     9061 cgtcgaagcc gccgtccgcg agcttcaccg caaacgcgag cacctgggcg ctgtgtggcg
     9121 tgtcgtagat cgtgaccagc ggacagcgca gcacctcggc gccgcgccgc tcgagaaggc
     9181 cgctgaatac ctcgagctcg cgcatctcgg gaaccgcgat tcgcttgcct ttgaggttcc
     9241 agtcgatctg ggctggaggt gtcatcgtgg gatccgcacg gcgcgagtgt aacccggcga
     9301 atgaggcttc tcgacgcccg atgtcctgtt cgggcccgca ccgggatcgc gcgggtcttg
     9361 cgcctgcgct agatcctggc tcccgcgacg ccgacggcgc cccaagtggt tctccagcga
     9421 tgcttgatga tggttagccc gaccagcgcc agcaccgcca gggacgcaaa cagcaggagt
     9481 cctgcctggt atgtgcccgt gagttgtttg gagagcccca ggctcgacgc caggaagaag
     9541 ccaccgacgc cgccggtcat gccgacgagg ccggtcatca cgccgatctc gtgacggaag
     9601 cgctgtggca cgagctggaa cacggcgccg ttacccatgc cgaacgcgaa catggcgccc
     9661 gcgaacagcg ccagcgcgcc gaacacgctc tgcgggtgaa agctgacgaa aaacagcgcg
     9721 agggcggccg ccccgtacat gatgctcagc gtcttgatgc cgccgatgcg atcggcgagt
     9781 gcgccgccga tgggccggac cagcgaaccg cagaacacgc aggccgcggt gaaatagccg
     9841 gcctgcaccg cgccgagacc gtagtcggtg ttgaagtaga tcgcgagcga tgaggcgagc
     9901 ccgacgaacc cgccgaacgt caccgcgtag aagaacatga accaccacgc gtcgcccatg
     9961 cggaacaccg cgagatactc gtggagtttt ttcggcggcg gcgtggtcgg gctgtccttg
    10021 gcggcaaaca gatagacgac gaaggcgagc gcgagcggca gaaccgcgag gcccagcacg
    10081 ttgttccagc cgacgaggac ggcaagcccg ggcgcgaaca gcgacgcgaa cacggtgccg
    10141 gagtttccgg cgccggcaat gccgagcgcg gtgccctggt actgcggcgg ataccagcgc
    10201 gaggcgagtg gcaatgcgac ggcaaatgac gcgccggcca cgccgagcac caggccgagc
    10261 agcagcgtgc cctgataact cttgatgcct accagccacg cggtagcgag accggcgatg
    10321 acgatgacct ggccgatgat gccggcgcgt ttgggtttga ggcgatcgac gagaatgccg
    10381 aagacgacgc gcagcaacgc acccgccagc acagggaggg cgaccatgaa gcccttctgt
    10441 gccgcgttga gcgacagctc cgcggacatc tgcacggcga gggcgccgag gagcacccag
    10501 accatgaagc tcatgtcaaa gtagaagaac gcggacagca aagtgggaag atgtcccgct
    10561 ttcaggaatt cacgattgat cactgcaccc ctcgacaaca cgacccaagt ccccgggact
    10621 atgcaaggag cggaccacgg gaccgccgac ggattgcagg tcggagtggg tcattccacg
    10681 caccagggca cccgcgccga caaccaggac tacgcggcgt gttatgtcgg accggcccgg
    10741 aatcagctaa ctatcggcct gctggccgcg ctggcggatg gcatgggggg cgccaaaggc
    10801 ggtcgtgtgg cggctgaatt ggcggtacgc ggtttcatcg acggttgtct cgggcagccg
    10861 tcgaccattg gcctgtcccg catcggcgcg cgggccgccg acgcggtcaa tcgctggatc
    10921 cacacgctcg gtcgctcgga tccgggcctc aacggcatgg cctgcacttt gagtgcactc
    10981 ctgctgtgcg ggaggggtac ccatctggtc catgttgggg attcgcgtgt ttatcgcatg
    11041 cgcgacaacg aactcacgct tctgactacc gatcacacgc tgggtgcgcc gggcactact
    11101 cacgccctga cgcgcgcggt cggtgcgcag gacagcctgc gcgtcgatca cttcaaggaa
    11161 accgcgcact tgcacgatcg ctacctgctc tgcagcgacg gcgtgcacgg cgcgctttcg
    11221 cagaagcaga ttcacgcgtt gctggcgcgc cgcgccgcgc cggacgagac cgcgcgcgag
    11281 ctggtcgcgc aggccgcggc gagcaccgat tcggataact gcacggcgat cgtgctcgat
    11341 atcctcgcat tgccatcgac gcagttcgcc gacatggagc tcgccatcgg cgaacagccg
    11401 ttgcgcgccg cgccgtcgag cggcgcggtg atcgacgact acgagctcgg cgagatgctc
    11461 gccgacggac agtacatgcg cgtgtttcgc gcccgcgatc accgcgccag cc
//
LOCUS       DATDEU010000751         3955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1312009, whole genome shotgun sequence.
ACCESSION   DATDEU010000751 DATDEU010000000
VERSION     DATDEU010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312009"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /gene="gidA"
                     /locus_tag="VIV63_17845"
     CDS             <1..611
                     /gene="gidA"
                     /locus_tag="VIV63_17845"
                     /inference="COORDINATES: protein motif:HMM:NF045129.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY6126522.1"
                     /translation="FTSRAEHRLLLREDNADLRLTPAGRELGLVDDARWAFFESKRDG
                     SARELARFETTRIKAADIPAAWSTRVLGDTTTRDALSAFDLLRRPEVSYDDVVELIGP
                     MNAVDDDRLPAQIRVQLEVAAKYSGYIERQEEEIARQRRNEETRLPVDLDYARVSGLS
                     NEVRQKLEQIRPATVGQAARVAGVTPAAVSILLVHLKKSRAA"
     gene            611..2227
                     /locus_tag="VIV63_17850"
     CDS             611..2227
                     /locus_tag="VIV63_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6126523.1"
                     /translation="MGRAVTRRELLAASLATLGVTACGRKDGSSGKQSAILLRGNGPD
                     PDSLDPHKARSTEAMVVLRDLFEGLTRLDPNAVTIPAAAESWTVSEDGRVYTFKLRPN
                     LRWSNAEPVVAEDFAAGLRRLVDPATASQYAEVIDVILNAPDIVAGRKPVDTLGVAAP
                     DTQTVVITLTSPALYLPGLMAHPSTAPLHRPSFAQHGQRFARPGDQVSNGAFVLKEWL
                     QGSYIRVERNPNYWGNAANKIDGVKFLQLADENAELRAYRAGELHCTYVLPRGQFDWI
                     KENLAAEMHVSPQLSTYYYGFNLDRELFAKNPKLRQALSMAIDRERLANSVLRMGELP
                     AYGWVPPGMYNYTAQTFDYAARTQAERITVARQLLAEAGYTREKPLRFELRYNTGEVH
                     TKVAVAVASMWKEALGVEAQLAAVEFKSLFDDIDRRNIDVYRLSWVGDYNDPYTFLQY
                     FKAGFGINLPHYSSPAYDALLEEASRQTDAARRRALLEQAERLMLADHPIIPLYYYVN
                     KHLVKPELKGWYDNVMNVVYSQDLEIAPRS"
     gene            2396..2701
                     /locus_tag="VIV63_17855"
     CDS             2396..2701
                     /locus_tag="VIV63_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126524.1"
                     /translation="MRNTAVGQGYGSSAYGPTQFQDDIDVAECALDEQLEQLQRHYAT
                     VSRAVSRARFEIELLEKRDDVSPHVLAQAKRQRAAAETRCTRLLTAIEALEDRLENA"
     gene            complement(2721..>3955)
                     /locus_tag="VIV63_17860"
     CDS             complement(2721..>3955)
                     /locus_tag="VIV63_17860"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY6126525.1"
                     /translation="RVTIAKVLDQIKPQLVTNPEDSGFYAPFKKMPASFDAVTKDRLT
                     AAAKTAIQTVAVPAFQRFDKFFRDVYLPASRDTVGIYDTPDGDLYYKNRMKFYTTIDN
                     PDATRIHNIGLEEVKRIRAEMEKTLEGINFLGTLDQFLSFIRNDPRFYYKTPEELMAA
                     YEKTARAIEPQLPKLFGKLPKQSFGIRPIPAASAPTTTTAYYQPPALDGSRKGNYYVN
                     LYKPESRPIWEIEALTAHESVPGHHLQIALSYELTGLPEFRRNAGYTAFVEGWALYSE
                     SLGYELGLYTDQFSKIGQLNYDMWRAVRLVVDTGMHQFKWTRDQAIYFFKQNTGKSQQ
                     DIENEVDRYISWPGQALAYKLGQLRIQALRAEAEKALGDRFDIRAFHDQLLGMGALPL
                     SVLDVEMRAWIAAQSARR"
BASE COUNT          740 a         1262 c         1252 g          701 t
ORIGIN      
        1 tgttcaccag ccgcgccgaa catcgcctgc tgctgcgcga agacaatgcc gatctgcgac
       61 tcacacccgc cggacgcgag ctgggcctcg tggacgacgc gcgctgggcg ttcttcgaat
      121 ccaagcgcga tggcagcgcg cgcgaactcg cccgcttcga gaccacgcgc atcaaggccg
      181 ccgacattcc cgcggcctgg tcgacgcgag tcctcggtga caccaccacg cgcgacgcgt
      241 tgtccgcgtt cgatctgctg cgccggcccg aagtttcgta tgacgacgtg gtcgagctca
      301 tcgggcccat gaacgcggtc gatgacgacc gccttcctgc gcagatccgc gtgcagctcg
      361 aagtggcggc caagtactcg ggctacatcg agcgtcagga ggaagagatc gcgcgccaac
      421 ggcgcaacga agaaacgcgt ctgccggttg atctcgacta cgcgcgcgtc agcgggctgt
      481 cgaacgaagt gcggcagaag ctcgagcaga tccggccggc cacggtcggc caggcggcgc
      541 gcgtcgccgg cgtcaccccc gcggcggtct ccatcctgct ggtccatctc aagaagtcgc
      601 gcgcggcctg atgggaaggg cggttacccg acgcgaactc ctggctgcgt cgctcgccac
      661 cctcggcgtc accgcctgcg gcagaaaaga cggctcgtcc ggcaaacaat ccgcgatcct
      721 cttgcgcggc aacggcccgg atccggattc tctcgatccg cacaaggcgc ggtccacgga
      781 agccatggtg gtgctgcgcg atctgttcga aggcctcacg cgcctggatc cgaatgcggt
      841 caccattccc gcggccgccg aaagctggac ggtcagcgag gacggccgtg tctacacgtt
      901 caaattgcgg ccgaatctgc gctggtcgaa tgccgagccc gtggtggccg aagacttcgc
      961 cgcaggcctg cgtcggctcg tggatccggc gacggcctcg caatatgccg aagtcatcga
     1021 cgtcatcctc aacgcgccgg acatcgtcgc gggacggaag cccgtggata ccctgggcgt
     1081 ggcggctccc gacacgcaga ccgtcgtcat cacgctgacg agtcccgctc tctatctacc
     1141 cggcctcatg gcacacccgt ccactgcgcc gctgcaccgc ccatcgttcg cgcagcacgg
     1201 ccagcgtttc gcgcgacccg gagaccaggt gtcgaacggc gcgttcgtgc tcaaggagtg
     1261 gctgcagggc agctacatcc gggtggagcg caatccgaat tactggggca acgccgcgaa
     1321 caaaatcgat ggcgtgaagt tcctgcagct cgcggatgaa aacgccgagc tgcgtgcgta
     1381 ccgcgccggc gaactgcact gcacctatgt gctgccgcgc gggcagttcg actggatcaa
     1441 ggagaacctg gccgccgaga tgcacgtgtc gccgcaactc agcacttatt actacggctt
     1501 caacctcgat cgcgagttgt tcgccaaaaa ccccaagttg cgccaggccc tgtcgatggc
     1561 catcgatcgc gagcgcctgg cgaactccgt gttgcgcatg ggcgagctgc cggcctacgg
     1621 ctgggtgccg cccggcatgt acaactacac ggcacagaca ttcgactacg cggcaaggac
     1681 ccaggccgaa cgaatcaccg tcgcacgcca gcttttggca gaggcaggtt atacgcgcga
     1741 gaagccgctg cgcttcgagc tgcgttacaa caccggtgaa gtccacacca aggtggcggt
     1801 ggccgtggcg tcgatgtgga aggaagcgct cggtgtcgag gcgcaactcg cggccgtgga
     1861 attcaagtcg ctgttcgacg atatcgaccg ccgcaacatc gacgtctatc ggctgtcatg
     1921 ggtgggcgat tacaacgacc cgtatacctt cctgcagtac ttcaaggccg gcttcggtat
     1981 caacctgccg cattattcga gccccgcgta cgacgcgttg ctggaagaag cgtcccggca
     2041 aacggatgcg gcccggcgcc gcgcgctgct cgaacaggcg gaacgcctga tgctcgcgga
     2101 ccatcccatc attccgctct actactatgt aaacaagcac ctggtgaagc ccgaattgaa
     2161 gggctggtac gacaacgtca tgaatgtcgt ctattcccag gacctcgaga tcgcgccgcg
     2221 cagctgaacg ctgtcgtccg ccgcagacgc tcccacgcgg ttgacaggcg atatctgaca
     2281 agggggtggg cgctcgtcca tcgccggtgc atcgagccgc cgacattcag cagcaataag
     2341 acttcgcgat gattgcacca acccggcaac agacggccgg gaaacaggag cagtcgtgag
     2401 aaacacagca gtcggtcagg gatacgggtc aagcgcctac ggtcccacgc agttccagga
     2461 cgacatcgat gtcgcggaat gcgcgctcga cgaacagctc gaacagttac agcgtcatta
     2521 cgccacggtc agccgcgccg tgtcgcgggc ccgtttcgag atcgaattgc tcgagaagcg
     2581 agatgacgtt tccccgcacg tgctcgcgca ggcgaagcgg cagcgcgcgg cagcggaaac
     2641 ccgctgcaca cggctgctca ctgcgataga agccctcgaa gaccggttgg agaacgcgtg
     2701 atgcgcagac agatcgacga ttaccgccgc gcgctttgcg ccgcaatcca ggcccgcatt
     2761 tcaacgtcca gcaccgacaa gggcaacgcg cccatcccga ggagctggtc gtggaatgcg
     2821 cggatatcga agcgatcgcc gagcgccttt tcggcttcgg cacgaagcgc ctggatcctg
     2881 agctgaccta acttgtaagc gagcgcctgg cccggccagg aaatgtagcg gtcgacttcg
     2941 ttttcgatgt cctgctggct cttgccggtg ttctgcttga agaagtagat cgcctggtcg
     3001 cgagtccact tgaactggtg catgccggtg tcgaccacga ggcgcaccgc gcgccacatg
     3061 tcgtagttca gctggccgat cttcgagaac tggtcggtgt agagaccgag ttcgtatccc
     3121 aggctctccg aatacaacgc ccatccttcg acgaacgcgg tgtagcccgc attacggcgg
     3181 aattccggca ggccggtgag ctcatacgac agcgcgatct gcagatgatg gccgggcacg
     3241 gattcgtgcg cggtcaacgc ttcgatctcc cagatcgggc gcgactccgg cttgtacaga
     3301 ttcacgtagt aattgccctt gcgcgagccg tcgagtgccg gcggctggta atacgccgtg
     3361 gtggtcgtgg gcgcgctcgc ggccggaatg ggacggatgc caaagctttg cttcggcagc
     3421 ttgccgaaca gcttcggaag ctgcggctcg atggctcgcg cggttttctc gtacgccgcc
     3481 atcagctctt cgggcgtctt gtaatagaac cgcggatcgt tgcggatgaa ggacagaaac
     3541 tggtcgagcg tgccgaggaa gttgatgccc tcgagcgtct tctccatctc cgcgcggatg
     3601 cgcttcactt cctcgagacc gatgttgtgg atacgcgtcg catcggggtt gtcgatggtc
     3661 gtgtagaact tcatgcggtt cttgtagtac agatcgccat ccggcgtgtc gtagataccc
     3721 accgtgtcgc gcgaggccgg tagatagaca tcgcggaaga acttgtcgaa gcgctggaag
     3781 gccggcaccg ccaccgtctg gatcgcggtc ttggcggcgg cggtcaggcg gtccttcgtg
     3841 accgcgtcga agctcgcggg catcttcttg aacggtgcgt agaagccgct gtcttccggg
     3901 ttcgtgacga gctgcggctt gatctggtca agcaccttcg cgatggtgac gcgcg
//
LOCUS       DATDEU010000752         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_729196, whole genome shotgun sequence.
ACCESSION   DATDEU010000752 DATDEU010000000
VERSION     DATDEU010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729196"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1155
                     /locus_tag="VIV63_17865"
     CDS             121..1155
                     /locus_tag="VIV63_17865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY6126526.1"
                     /translation="MKTRRYETPVAPARQTPPKVAEVEAAPPAKVQQVEAGERGAGQR
                     LDNFLGRILKDVPKTHIFRVIRKGEVRVNGKRAKPDQRLLASDIVRVPPVRTGAAAPP
                     RRASPAMIEGLTGAIVYEDPRLLVIDKPAGIAVHGGSGVSFGVIEALRSARPDETLEL
                     VHRIDRDTSGLLLVSRKPAALRTLHALMRDGLVEKRYLALVKGKWELGQKRIDVPLRT
                     DIRVGGERTVKAHESGKEAVSVFKPVQFFGKRASLVEVSLETGRTHQIRVHASHAGYP
                     LAGDEKYGEEEFNEKMKETGLKRMFLHAHHLSFTWPDTGVEFSASAPLPADLAAVIDA
                     LNPPKAKKSS"
     gene            complement(1152..1748)
                     /locus_tag="VIV63_17870"
     CDS             complement(1152..1748)
                     /locus_tag="VIV63_17870"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY6126527.1"
                     /translation="MSPNAPTRPLILASTSRYRASLLQRFGLPFTPHNPAVDETERHG
                     ESPRARAFRLSEAKAEAVAALFPQAIVVGGDQVPATSTTILNKPGNEANCREQLKLLS
                     GSTAEFYTACTVRCLETGVKLSHVDTTAVKMRPLADAEIDRYIEREKPFDCAGGFKAE
                     ALGIVLFERIDTEDPTAIIGMPLIWLAGALRAVGFAVP"
     gene            1816..2346
                     /locus_tag="VIV63_17875"
     CDS             1816..2346
                     /locus_tag="VIV63_17875"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY6126528.1"
                     /translation="MSLGWSRRAPVDTLVGTEAVVDFSIPLTELPRVAHELVSADGEA
                     KGQVRFSRQAGQAVADLEVDAQPEVVCQRCMQPMRWPVKVESRIALVSDYNAADRVPD
                     GMEVFLVEAESVSVRDLVDEEILLALPHVARHEEGSECADSAMQLSGQDAESEEDSDP
                     QVQKPFAQLGELLKRK"
     gene            2372..2566
                     /gene="rpmF"
                     /locus_tag="VIV63_17880"
     CDS             2372..2566
                     /gene="rpmF"
                     /locus_tag="VIV63_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY6126529.1"
                     /translation="MPVQKSRKTPSKRGMHRSHDGLTKPALSVDPQSGETHLRHRITP
                     DGFYRGKRVIEKPAEVETEE"
     gene            2600..>3049
                     /locus_tag="VIV63_17885"
     CDS             2600..>3049
                     /locus_tag="VIV63_17885"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY6126530.1"
                     /translation="MPSIAVDVMSGDSGAPECIPGALRALVTDTALELILVGDPTVIE
                     PALAHAPASVRSRVSVHPAATVVGMSEHPREAIRRRKDSSMRIAIDLVKEGRAGAMVS
                     AGNTGALTAMAHFVLKTVGPVERAPIMSSVPARNGGHTHILDLGANTK"
BASE COUNT          569 a          926 c         1045 g          509 t
ORIGIN      
        1 actaacatgc ggcgcgttcg agtcgtctta ttacagagtg cgatcggcgg tgccgcggca
       61 ttgcgtcgtc acacacaagc tggggacgac gacggcgcgc atcatagccc gagaccctta
      121 gtgaaaacaa gacgttacga aactcctgtg gcgcctgcgc gtcagactcc tccgaaagtg
      181 gccgaagtgg aggcggcgcc gccggccaaa gtgcagcagg tggaagccgg tgagcggggc
      241 gcgggtcagc gtctcgacaa ctttctgggc cgaatcctca aggatgtgcc caagacgcac
      301 atcttccgcg tgatccgcaa aggcgaggtg cgcgtaaacg gcaagcgagc caagcccgac
      361 cagaggttgc tggcaagcga catcgtccgg gttccgcccg tgcggaccgg cgccgcggca
      421 ccgccgcgcc gggcgtcacc ggcaatgatc gagggcctga ccggcgccat cgtgtacgaa
      481 gatccgcggc tgctggtcat cgacaagccc gccggcattg ccgtgcacgg cggcagtggt
      541 gtgagctttg gagtcatcga ggcactgcgc tccgcgcggc cggacgagac cctggaactg
      601 gtgcatcgga tcgatcgcga tacgagcggc ctgctgctcg tgtcgcgcaa gcccgcggcg
      661 ctgcgcacgt tgcatgcgtt gatgcgcgat gggctggtcg agaagcgcta cctggcgctg
      721 gtgaagggca agtgggagct cgggcagaag cgcatcgatg tgccgctgcg caccgacatc
      781 cgcgtgggcg gcgagcgcac ggtgaaagcc cacgaatccg gaaaggaggc cgtgagcgtc
      841 ttcaagccgg tgcagttctt cggcaagcgt gcgtcactcg tggaggtttc gctcgagacc
      901 ggccgcacgc accagatccg cgtgcatgcc tcacatgcag gctatccact ggcgggcgac
      961 gaaaaatacg gcgaggaaga gttcaacgag aagatgaagg agacgggcct caagcgcatg
     1021 ttcctgcacg cgcaccacct gagcttcacg tggccggaca cgggcgtgga attcagcgcc
     1081 agtgcgccgt tgccggccga cctggccgcg gtcatcgacg cgctgaaccc gccgaaagcg
     1141 aagaaatcta gttagggaac ggcgaagccg acggcgcgca acgcgccggc gagccagatg
     1201 agcggcatgc cgatgatggc cgtggggtct tcggtgtcga tgcgctcgaa cagcacgatg
     1261 ccgagcgctt ccgccttgaa tccgccggcg caatcgaaag gcttctcgcg ttcgatgtaa
     1321 cggtcgattt ccgcatcggc cagcgggcgc atcttcacgg ccgtggtgtc cacgtgcgaa
     1381 agcttcacgc cggtttcgag gcagcggacg gtgcaggccg tatagaactc ggccgtcgac
     1441 cccgacagca acttgagctg ttcgcggcaa ttggcctcgt tgccgggttt gttgaggatg
     1501 gtggtgctgg tggccgggac ctggtctccg ccgaccacga tggcctgggg aaacagggcg
     1561 gccaccgcct cggctttggc ttcagacagc cggaaagccc gcgcacgcgg cgattcgccg
     1621 tgccgctcgg tttcatccac ggccggattg tgcggcgtga agggcaggcc gaagcgctgc
     1681 agcaggctgg cgcggtatcg ggaggtggaa gccaggatca gcgggcgcgt cggcgcattg
     1741 gggctcataa aaacaatggc ttagagttaa tgttgttttg acttggcggg atgcggtccc
     1801 tatcatacgc gccccatgtc actgggctgg tctcgtcgcg ctcctgtgga caccttggta
     1861 ggcaccgaag cggtggtcga tttctcgatc ccgctaaccg agttgccccg ggtggctcat
     1921 gagttggtga gcgccgatgg cgaagcaaag gggcaggtgc gcttctcgcg gcaggccgga
     1981 caggccgtcg cggatctcga ggtcgacgcg cagcccgaag tggtttgcca gcgatgcatg
     2041 caacccatgc gctggccggt gaaggtggag tcgcgcatcg cgctcgtcag cgattacaac
     2101 gcggcggatc gcgtgcccga cgggatggaa gtatttctgg tcgaggccga aagcgtcagc
     2161 gttcgggacc tcgtcgatga agagattctg ctcgcgctgc cacacgtggc gcggcacgaa
     2221 gaaggaagcg agtgtgcgga cagtgcaatg cagctgtccg gacaagatgc cgaatccgag
     2281 gaggattcgg atccgcaggt tcaaaaaccg tttgcacagt tgggcgaact gttgaaacgc
     2341 aagtagtaag tcgcccacga ggattggttc catgcccgtt cagaaaagtc gtaaaacgcc
     2401 gtccaagcgc ggcatgcatc gttcgcacga tgggctgacg aagcccgcgt tgtccgtcga
     2461 tccgcagtcg ggtgaaaccc acctgcgtca tcgcatcact cccgacggtt tttatcgcgg
     2521 caagcgcgtc atcgaaaaac cggccgaagt cgagaccgaa gaatagttgc ggagcggggc
     2581 gccgccccgc tcgtaacgca tgccgtcgat cgccgtcgat gtgatgagtg gcgatagcgg
     2641 tgcgccggag tgcatacccg gagcgctgcg cgccctcgtc accgataccg ccctcgagtt
     2701 gatactcgtt ggcgacccaa ccgtcatcga acccgcgctg gcccatgcgc cggcgtcggt
     2761 gcgttcgcgc gtgtccgtgc atcccgcggc cacggtggtg ggcatgtccg aacaccctcg
     2821 cgaggcgatc cgccgacgca aggactcctc gatgcgcatc gccatcgacc tggtgaagga
     2881 aggtcgtgcg ggcgccatgg tcagcgccgg caacaccggt gcgctgaccg ccatggcgca
     2941 cttcgtcctg aagaccgtgg ggcccgtcga gcgggcgccc atcatgtcgt cggtgccggc
     3001 gcgcaacggt ggacacacgc acatcctcga tctcggcgcg aacaccaag
//
LOCUS       DATDEU010000753         3803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_435303, whole genome shotgun sequence.
ACCESSION   DATDEU010000753 DATDEU010000000
VERSION     DATDEU010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435303"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..2037
                     /locus_tag="VIV63_17890"
     CDS             142..2037
                     /locus_tag="VIV63_17890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6126531.1"
                     /translation="MTTEQALAESEARFRALVTASSDVVFRMSADFRELRQLEGRMFI
                     ETSEVPNRDWFERYVVPEDRERVLCAIADAINRKCAFETEHRVFRADDGIGWVHARSV
                     PMLDADGEIREWIGMASDITPRKRVEQDFVNQRRMYEAILTNTPDLAYVWDLDHRFIY
                     ANEGLLRMWGKTWDEAIGKNCLELGYEPWHAEMHDREIDQIAATRQPLRGEVPFNGAF
                     GRRHYDYLLVPVIGANGEVEAVAGTTRDVTERKQLEGSLIEGDRRKDEFLATLAHELR
                     NPLAPIRNAVHLLKSGNPTGDSVRMAREIIDRQVQHMVRLVDDLLDVTRITVGQVNLR
                     NERISLRRVIDDALEAAAPAIENGNHRLVVDLPGTPVQLEGDSTRLSQVFQNLLDNAA
                     KYTPPNGTITLRAEQRGDQVIVSVRDSGIGISRDAQSRVFELFTRVHPNDRIKTSGLG
                     IGLALAKKLVALHLGDIEVRSEGQGMGSEFIVTLPALPAAPAAVPSAENAATPGKSGN
                     SRRILVVDDNRDAAESLGMLLEMGGCTVAIAFDGPQALMTIESFKPDVALLDIGMPGM
                     DGYELARRIRASQIGRNLVLVALTGWGQADDKKRATEAGFDQHLTKPVDPDLLEQIIN
                     FGHSAAA"
     gene            2114..3766
                     /gene="pgm"
                     /locus_tag="VIV63_17895"
     CDS             2114..3766
                     /gene="pgm"
                     /locus_tag="VIV63_17895"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEY6126532.1"
                     /translation="MTSTPKINPLAGKPAPANLLVDLPKLITAYYTLTPDVSVATQRV
                     AFGTSGHRGVSFQSSFNEAHVLAMTQAICSYRKQEGITGPLFLGFDTHALSAPAFASA
                     LEVLAGNAVDVMIAKDDEYTPTPALSHAILTYNRGRTSALADGIVITPSHNPPDSGCF
                     KYNPPNGGPADTQITGWVEAEANRLIAANLQGVQRVTLANARRASTTHAHDYLNSYVS
                     DLGNVIDMDAIRGANIRMGVDPLGGAGVHYWAAIADRYKLNLTVVNDAVDATFRFMSV
                     DWDGKIRMDPSSPHAMNSLIGLKDRFDIAFACDTDHDRHGIVTRSAGLLPPNHYLAVS
                     IDYLFQNRPGWRADAGIGKTAVSSQMIDYVAAKLKRRLYEVPVGFKWFVDGLFDGSLG
                     FVGEESAGAAFLRKDGGVWTTDKDGMAPALLSAEMTARTGRDPGEAYRALTREFGEPL
                     FERIDAAATPAQKKLLANLKPEQVLSKELAGGRIQGILTKAPGNGASIGGLKVVADGG
                     WFAARPSGTEDVYKIYAESYRSADHLKRIQSEAQQIVTAALG"
BASE COUNT          727 a         1257 c         1225 g          594 t
ORIGIN      
        1 atttccgggg ctggccgggc agttgaaggg cgggcatgga ggaaccggaa gaaggctgta
       61 ggagtcctcc taccttttcc cgtccacctc gcggctgttg catcctgtac tcctagtatg
      121 gaacggcagg aaatggcatc gatgacgact gagcaggcgc tggcagaaag tgaggcgcgt
      181 ttccgcgcgc tggtgaccgc cagctccgac gtggtcttcc gcatgagcgc ggatttccgc
      241 gagctgcgcc agctcgaagg acgcatgttc atcgagacgt cagaggttcc caaccgcgac
      301 tggttcgaac gctatgtcgt gcccgaagac cgggagcgcg tgctctgcgc gatcgccgac
      361 gccatcaacc gcaaatgcgc cttcgagacc gagcaccgcg tgtttcgcgc ggacgacggt
      421 atcggctggg tgcatgcgcg ctccgtgccg atgctcgatg ccgacggcga gatccgcgag
      481 tggataggca tggcgagcga catcacgccc cgcaagcgcg tggagcagga cttcgtcaac
      541 cagcggcgca tgtacgaggc gatcctcacg aatacgcccg acctcgccta tgtctgggat
      601 ctcgatcatc gcttcatcta cgcgaacgaa ggcctgctgc gcatgtgggg caagacctgg
      661 gacgaggcga tcggcaagaa ctgcctcgaa ctcggctacg agccctggca cgccgagatg
      721 cacgaccggg agatcgacca gatcgccgcc acgcgccagc cgttgcgcgg cgaagtgccg
      781 ttcaacggcg cgttcggccg ccgtcattac gactatctac tggttccggt catcggcgcc
      841 aacggcgagg tggaggcggt ggccggcacc acgcgtgacg tcaccgaacg caagcagctc
      901 gaaggctcgc tgatcgaggg cgaccggcgc aaggacgagt tcctggcgac gctggcccac
      961 gaactgcgca atcccctggc gccgatccgc aatgcggtac acctgctgaa gtccggcaat
     1021 ccgacgggcg acagcgtgcg catggcgcgc gagatcatcg atcggcaggt gcagcacatg
     1081 gtcaggctgg tggacgacct gctggacgtc acccgcatca ccgtgggcca ggtgaacctg
     1141 cgcaacgagc gcatcagcct gcgccgcgtg atcgacgacg cgctcgaggc agcggcgccg
     1201 gcaatcgaga atggcaacca ccggctggtc gtcgacctgc ccggcacgcc cgtgcagctc
     1261 gaaggcgatt ccacgcggct gtcgcaggtt ttccagaacc tgctcgacaa cgccgccaag
     1321 tacacgccgc ccaacggcac catcacgctg cgcgccgagc agcgcggcga ccaggtcatc
     1381 gtgtccgtgc gcgattcagg catcggaatc tcgcgcgatg cgcagtcccg cgtcttcgaa
     1441 ctgttcacgc gcgttcaccc gaacgatcgc atcaagacca gcggtctcgg catcggactg
     1501 gcgctcgcga agaagctcgt agcgctgcac ctgggtgaca tcgaagtgcg cagcgagggt
     1561 caggggatgg gtagcgaatt catcgtgacg ctgccggcgc tgccggccgc gcccgccgcc
     1621 gttccgagcg cggagaacgc cgccacgccc gggaagtccg gcaacagccg gcgcatcctg
     1681 gtcgtcgacg acaatcgcga tgcggccgag agcctgggga tgcttcttga aatgggtggt
     1741 tgcaccgtgg ccatcgcatt cgacggccca caggcgctga tgacgatcga gagtttcaag
     1801 cccgacgtcg ctctgctcga catcggcatg ccgggcatgg acggctatga actcgcccgc
     1861 cgcatccgcg cctcgcaaat cggccgcaac ctggtgctgg tggcgctcac cgggtgggga
     1921 caggccgacg acaagaagcg tgcgacggag gctggcttcg atcagcatct gaccaagccc
     1981 gtcgatccgg acctgctgga gcagatcatc aacttcgggc attccgccgc cgcctgaaat
     2041 ccgtattcga gttctcgcgc ggtgggtgca tcatgggcgc acctgccaat gcctggctgc
     2101 gcccgaggac tccatgacgt cgacgccgaa aatcaatccg ctcgcgggca aacccgcgcc
     2161 ggcgaacctg ctggtcgatc tgccgaaact gattactgcc tactacacgc tgacccccga
     2221 tgtatcggtg gccacccagc gtgtggcgtt cggcacttcg gggcaccgcg gggtttcttt
     2281 tcagagcagt ttcaacgaag cgcacgtgct cgccatgacg caggccatct gcagctaccg
     2341 caaacaggaa ggcatcaccg gcccgctgtt tctcggcttc gacacgcacg cattgtccgc
     2401 accggcgttc gccagtgcgc tcgaagtcct cgcgggcaat gcggtggacg tcatgatcgc
     2461 gaaggatgac gaatacacgc ccacgccggc gctgtcccac gccatcctga cgtacaaccg
     2521 cgggcgcacg agcgcgctcg cggacggcat cgtgatcacg ccctcgcaca atcctccgga
     2581 cagcggctgc ttcaagtaca acccgccgaa cggtggcccc gccgacacgc agatcaccgg
     2641 ctgggtcgag gcggaagcca atcgactgat cgccgcgaac ctgcagggcg tccagcgcgt
     2701 cacgctcgcc aacgccaggc gcgcctcgac cacccacgcg cacgactatc tgaattccta
     2761 cgtgagcgat ctcggcaatg tcatcgacat ggacgccatc cgcggcgcga acatccgcat
     2821 gggggtcgat ccgctcggcg gcgcaggcgt ccactactgg gccgcgatcg cggaccgcta
     2881 caagctgaat ttgaccgtgg tcaatgatgc ggtggacgcc accttccggt tcatgagcgt
     2941 ggactgggac ggaaagatcc gcatggatcc gtcctcgccg cacgcaatga acagcctcat
     3001 aggcctcaag gatcgcttcg acatcgcctt cgcctgcgat accgatcacg accggcacgg
     3061 catcgtcacg cgcagcgcgg gactgctgcc gccgaatcac tacctcgcgg tgtcgatcga
     3121 ctatctattc cagaaccggc ccgggtggcg agcggacgcg ggcatcggca agaccgcggt
     3181 cagcagccag atgatcgact acgtcgccgc gaagctgaaa cgccggctgt atgaagtgcc
     3241 ggtaggcttc aaatggttcg tcgacggact gttcgacgga tcgctcggat tcgtcggcga
     3301 ggaaagcgcg ggcgcggcct tcctgcgcaa ggacggcgga gtctggacca ccgacaagga
     3361 cggcatggcg cccgccctgc tctccgcgga aatgactgcg cgcaccgggc gcgatcccgg
     3421 cgaagcgtat cgcgcgctca cgcgtgagtt cggcgagccg ctgttcgaac gcatcgacgc
     3481 tgccgcgacg ccggcacaga aaaagctgtt ggccaacctc aagccggaac aggtgctgtc
     3541 gaaagagctg gccggcggca ggatccaggg catcctcacg aaagcgccgg ggaatggcgc
     3601 atcgatcggc ggactcaagg tcgtggcgga tgggggctgg tttgccgcaa gaccctccgg
     3661 caccgaagac gtctacaaga tctatgcgga aagttaccgc agcgccgatc atctcaagcg
     3721 catccagtcc gaggcccagc agatcgtgac ggcggcgctg ggatagcagt gaccaggccc
     3781 cctgaaaacc ggcgcggaca acg
//
LOCUS       DATDEU010000754         6909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1895205, whole genome shotgun sequence.
ACCESSION   DATDEU010000754 DATDEU010000000
VERSION     DATDEU010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895205"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..331)
                     /locus_tag="VIV63_17900"
     CDS             complement(194..331)
                     /locus_tag="VIV63_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126533.1"
                     /translation="MNLTETSLRNPAGVLVAILMVALLGVFALVPILIKKFKSKSAAA
                     S"
     gene            complement(484..2019)
                     /locus_tag="VIV63_17905"
     CDS             complement(484..2019)
                     /locus_tag="VIV63_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126534.1"
                     /translation="MRKIRSPLAVAVSAACLGLSANAALGADAETAITIYSSAQPGAI
                     SPEFYRPIPGQGVPNAMTVPGYALVRDDRDVKLKQGRAQLSFTDVAALIDPTTVTFAS
                     LTDPSTRVLEQNFQFDLVSTQKLLLKFIDKQITVDKLNGNTVTPVTGTLLSAIDGIVL
                     RGTDGSIYSLPSYTSVRFPDLPGGLNTRPTLVWDIQSPTGGTHKARVTYQTGGITWWA
                     DYNLIFNEGADANSGLLDLSAWVSIINQSGATFSDAKLKLIAGDVNRVQPEMAMDMRR
                     EVLMKAQAAPANAGGFVQKDFFEFHLYTLGRKTTLPNNSTKQIELFDQAKQIPAKKVL
                     LYYGAVQPFFYPNPYTDRNMGVDMNKKVDVYLEFKNDKQFGLGVPLPAGRLRVSQLDK
                     ADDSLEFIGEDTIDHTPKDEQVRVKLGSAFDVVGERRQVNFSVDSAGKVMEEEIEVKL
                     RNHKSQPVEVVVKENLYRWSNWKILTQTHKFDKEDARTISFPVKVAKDGETVIRYRVR
                     YTW"
     gene            2408..3097
                     /locus_tag="VIV63_17910"
     CDS             2408..3097
                     /locus_tag="VIV63_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126535.1"
                     /translation="MDREFIIKNQIVERYLAGRLPPKGVTDFERLIRSRPQLIEELGL
                     PEKVNAGLRLLDAAGIAEPWAEKPKKVWEKPVFTMGAAALAIVALIGCAVLATQVSSR
                     NTTIARQKQENIERTIAPSTLKRTILVGVARNGPPAQSTLTIRHGEFAELKADMSWAR
                     YSAFKVSIDRVNQGRVAVIDAMEKDSNGHLRLSLNAGAFGPGDYMVTIEGLNLRRQSL
                     PVAWFRITVGR"
     gene            complement(3116..4771)
                     /locus_tag="VIV63_17915"
     CDS             complement(3116..4771)
                     /locus_tag="VIV63_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEY6126536.1"
                     /translation="MAEGKPVKRRSMVGWYDPRVLAHSAYKLVIANIFGRHSDTRLIE
                     ALASQPQNEFDYTASEGDFWFDYAADVGDGWNPTYAIADAIARPELTVDVHGKPEKTQ
                     AGRLLIFGGDEVYPYPTRNEYDVRTETPYKLAFAGRAHPDVFAIPGNHDWYDSLVAFS
                     RLFCRPERGFADCTTRQTRSYFALKLPANWWLLAIDLQLGADLDEPQVQYFQKVASRM
                     DDSARLIFCVPEPRWILEDAYPSHASYEELSSTRFLEEKVFKRKARVFLTGDLHFYKR
                     HESAEGIQKITSGGGGAFLHPTHAPCTKELRNGFVQRAAYPDEKTSRRLTWRNFLFPA
                     INPKSGWLYAFLYAMSAWLASASLEASDVIDVRTALAAAMNAAIRDPLNGMWLVSVIG
                     AFIFFTDTHIRSWRILGGACHAVMHLAAAFAVGWLALLLTANVIELSYGSIAQLLLSG
                     LITFVLGGPVGAFILGVYLFISIRIFGRHGNEAFSSLRIQDFKQWLRLRIDAAGVLTI
                     YAIAIDRVPRRWREAQRNGKPTIEANDARATAPRLIDRVEIRP"
     gene            complement(4771..>6909)
                     /locus_tag="VIV63_17920"
     CDS             complement(4771..>6909)
                     /locus_tag="VIV63_17920"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6126537.1"
                     /translation="MLSDEPFGEPQVSTGNPGRGEVWPDSRLAAALGAAVGTDLNIGT
                     GTFRVSRILITRPDQGATFLELAPSLIMNEDDLASTQLVQPGSRLRRAQLFAGQRDEI
                     ASFKIWLEANKKPNEEIEDVEDSAPQIKAAIDRSARFLSIASLVAVLLCAIAVAMAAR
                     RYVHRHLDSVALLKTLGATRAFTLAVSLIQLTMVGVIAAVLGSLLGFGAQAWLVKALA
                     GLLRGDLPAPGLAPLGIGFLTAIAVLGGFALPPLLQLSRTPALRVLRRDVGPPQPLVV
                     LAFGPAVLAIAFLIYWVLRDQKTFFAFVIGLSGFVLVTALAGWALVKLTTRFRGGVGV
                     SWRYGIANLGRRRTESVVQLTAFALGIMMLLLLAVVRNDLLNDWRRSLPADTPNFFFI
                     NIPPNEHQAFTTFLEERGAQHARARPMVRARMTQINGTPVDDIKFATRRGREFANRDQ
                     NITWAETLGNDTVVTDGKWFTPEDFGKPLVSVSTEYMEEMNLKLGDELQFDIAGETHS
                     AKISSVRDVKWDSFQPNFFLMFAPGMLDGSQGTWMTAAHLTATNPKTISDLVRRFPSV
                     SVFNVDDLLRQVRSVIDKAVAAVQSVFLFTLMAGLVVLIAAVQASREERRYESAMLRT
                     LGAQRSTVLKGLLAEFAALGVLSGALAAAGASIAGVYIATQVLQIPYSADPWVWFYGL
                     VGGGLLVCAAGWLATRSVVNQPPVLTLRGG"
BASE COUNT         1309 a         2151 c         2231 g         1218 t
ORIGIN      
        1 ggccttgtgc catcgcgctg gagagcgcga cgagaaaggc caagccgagg ccgacaccgg
       61 tcaaaaacgg gcgccatgcg gcgaggcgtg accggcgacc gcggggatga acggtggaat
      121 tggacagtga atccggcgga cgagcggcca tgacgaggca tcctgaacag aacattcaaa
      181 gtccctcccg gttctacgac gccgccgcgc ttttggattt gaacttcttg atcaggatcg
      241 gtaccagcgc gaacacgccg agcagcgcga ccatgaggat ggccaccagc acgcccgcgg
      301 gattgcgaag cgaggtctcg gtgagattca ttgcgtgctg ccctgccgcg ccggtattga
      361 cgccacgata ggggcttgga tgcggggaaa gccgcggggt ttacacgctt cgtcgcggcg
      421 ctggatcgtt tgggcgtaga gaaggggcgc cggctcgaga accggcccgg cgccctatct
      481 ttcttaccag gtgtaacgca cccggtacct gattaccgtc tccccatcct tcgccacctt
      541 caccgggaag ctgatggtgc gggcgtcttc cttatcgaac ttgtgcgtct gcgtcaggat
      601 tttccagttg ctccagcggt agaggttttc cttcaccacg acctccacgg gctgcgactt
      661 gtggttgcgc agcttcactt cgatctcctc ttccatcacc ttgcccgcgc tgtcgacgct
      721 gaagttcacc tgccggcgct cgccgacgac gtcgaacgcg gatccgagct tcacacgaac
      781 ctgttcatcc ttcggcgtgt gatcgatggt gtcttcgccg atgaactcga ggctgtcatc
      841 ggccttgtcg agctgggata cacgcagtcg gccggcgggc aacggaacgc cgaggccgaa
      901 ctgcttgtcg ttcttgaact cgaggtacac gtccaccttc ttgttcatat ccacgcccat
      961 attccggtcg gtatacgggt tcggatagaa gaacggctgc accgcgccgt aatacagaag
     1021 caccttcttc gccggaatct gtttcgcctg gtcgaagagc tctatctgct tggtggagtt
     1081 gttcggcaac gtggtcttgc ggccgagcgt gtacagatgg aactcgaaga agtccttctg
     1141 cacgaagcca ccggcattgg ccggggcggc ctgcgccttc atcaacacct cgcgcctcat
     1201 gtccatggcc atctcgggct gcacccggtt cacgtctcct gcgatgagct tgagcttggc
     1261 atcggagaac gtggctcccg actggttgat gatgctcacc cacgcggaca gatccagcag
     1321 gccgctgttg gcgtccgcgc cttcattgaa gatgaggttg taatcggccc accaggtgat
     1381 accgccggtc tgataggtga cgcgcgcctt gtgcgtaccg cccgtcggcg actggatgtc
     1441 ccataccagc gtcgggcgcg tgttcaggcc gccgggcaga tcgggaaaac gcaccgaggt
     1501 gtaggacggc agcgagtaga tagagccgtc agtcccgcgc agcacgatgc cgtcgatggc
     1561 cgagagcagc gttccggtga ccggcgtcac ggtgttgccg ttcagcttgt ccaccgtgat
     1621 ctgcttgtcg atgaatttga gcagcagctt ctgcgtgctc acgaggtcga actggaaatt
     1681 ctgctcgagc acgcgcgtgg acgggtccgt gagcgatgcg aacgtgaccg tggtcggatc
     1741 gatgagtgcc gcgacgtcgg tgaacgacag ctgcgcccgc ccctgcttga gtttcacgtc
     1801 gcggtcgtcg cgcaccagcg cgtagccggg aacggtcatc gcgttcggca cgccctggcc
     1861 gggaatcggg cgatagaact cgggcgagat cgcgccgggt tgggcggagc tgtagatggt
     1921 gatggcggtt tcagcgtcgg cgccgagcgc cgcgtttgcg gaaagcccca aacaggcggc
     1981 ggacacggca acggcgagcg gactacgaat cttgcgcatg taaggcgaac ctcattgaaa
     2041 gaacacaaag gccaacgttt gcagtgcggc gggggtatta gacccgccgc ctgcgggaaa
     2101 ctgcgaacca cgtcgcaggt tctccgggaa ccggtttgcg gaatgtgaag cggcgcagcg
     2161 gcttccagac ataagaatcg cagcataggc gcgtgcgatc ctgtaggacc aacggcagat
     2221 accagcccga gtggcgcctc gcttttttgc gacgcatgcg caccggtttc ggaagccgcg
     2281 aagtccgttc gtccctgaat ctgcggaact ttgaaccacg atggaagctg cgagtcgtat
     2341 cgaatcctct ggtgtgaacc aggcgccagc cccggggctt tctttgcccg gcacgcctgc
     2401 gcccgcgatg gatcgtgaat tcatcatcaa gaaccagatc gtcgagcgat acctcgccgg
     2461 ccgcctgccg cccaagggcg ttaccgattt cgaaagactg atccgttcac gtccgcaact
     2521 catcgaagaa ctcggcctcc cggagaaggt gaatgcaggt ctgcgtctgt tagacgcggc
     2581 cggcatagca gaaccttggg ccgagaagcc gaaaaaggtc tgggagaagc ccgtgttcac
     2641 gatgggcgcc gcggcgctcg ccatcgtggc gctgatcgga tgcgcagtgc tggcgacgca
     2701 ggtgtcgtca cgcaacacaa ccatcgcccg gcagaagcag gaaaacatcg agcgcacgat
     2761 cgcgccgtcc acgctcaagc ggacgatcct cgtcggcgtt gcccgcaacg ggccgcctgc
     2821 gcagtcgacg ctcaccatcc gccatggcga attcgcggaa ctgaaagccg acatgtcctg
     2881 ggcgcgctac agcgcattca aggtgtcgat cgatcgggtg aaccagggtc gtgtggcggt
     2941 catcgacgcc atggaaaagg attccaacgg gcatctgcgt ttgtcattga atgccggcgc
     3001 cttcggcccc ggagactaca tggtgaccat cgaaggtctg aacctgcgcc gccagagcct
     3061 gccggtcgcg tggttccgca tcaccgtcgg acgctgaagc cgccccgctg ccgattcagg
     3121 ggcggatctc aaccctgtcg atgagccgcg gtgcggtcgc gcgcgcgtcg ttcgcctcga
     3181 tcgtcggctt gccgttcctt tgcgcttcgc gccagcgccg tggcacccgg tcgatcgcga
     3241 tcgcgtagat cgtgagaaca ccggccgcgt cgatgcgcag gcgcagccac tgcttgaaat
     3301 cctggatgcg cagcgacgaa aaggcttcgt tcccatggcg accgaatatg cgtatcgata
     3361 tgaatagata gacgcccagg atgaaggcgc ccaccggacc acccagcacg aaggtgatga
     3421 ggccggagag cagcagctgc gcaatgctgc cgtaagagag ctcgatgacg ttcgcggtga
     3481 gcagcaaggc aagccagccg accgcaaatg ccgcggcgag atgcatcacc gcatggcagg
     3541 cgccgcccag gatgcgccac gaacggatat gcgtgtcggt gaagaagatg aaagcgccga
     3601 tgacgctgac cagccacatt ccattcagcg gatcacgaat ggccgcgttc atcgcggcgg
     3661 ccagcgccgt gcgcacgtcg atgacgtctg aggcttcgag actcgccgag gccagccacg
     3721 cggacatggc gtagaggaac gcgtacagcc agcccgattt cggattgatg gccggaaaca
     3781 ggaagtttcg ccacgtgagg cgccgcgacg tcttctcgtc tggataagcc gcgcgctgca
     3841 cgaagccatt gcgcaattct ttcgtgcagg gcgcatgcgt gggatgcagg aacgcgccgc
     3901 ctcctcccga cgtgatcttc tggattccct cggcgctctc atgacgcttg tagaagtgca
     3961 ggtcgccggt gaggaatacg cgcgccttgc gcttgaacac cttctcttcg aggaaacgcg
     4021 tactcgacag ctcttcgtaa ctcgcgtgac tcgggtaggc gtcttcgagg atccagcgcg
     4081 gctccggtac gcagaagatg aggcgcgcgg agtcatccat gcgcgaagca actttctgga
     4141 aatactgaac ctgcggctcg tcgagatcgg cgcccagctg caggtcgatc gccagcaacc
     4201 accagttggc gggtagtttg agcgcgaaat agctgcgggt ctggcgcgtg gtgcagtcgg
     4261 cgaagccacg ctcgggccgg cagaacaggc gggagaacgc caccaggctg tcgtaccagt
     4321 catggttgcc gggtatcgcg aacacgtcgg gatgagcacg gccggcaaac gccagcttgt
     4381 aaggcgtttc ggtgcgcacg tcgtattcgt tgcgcgtcgg atagggatag acctcatcac
     4441 cgccaaagat gagcaggcgg ccggcctggg ttttttccgg cttgccatgc acgtcgaccg
     4501 tgagttcggg acgcgcgatg gcatccgcga tggcgtaggt ggggttccag ccgtcaccca
     4561 cgtcggcagc gtagtcgaac cagaaatcgc cttccgacgc cgtgtaatcg aattcgttct
     4621 gcggctgact cgccagagcc tcgatgaggc gggtgtccga gtggcgaccg aagatgttgg
     4681 cgatgaccag cttgtacgcc gaatgcgcaa ggacgcgcgg gtcgtaccag cccaccatcg
     4741 accggcgttt gaccggcttg ccttcagcca tcacccaccc cgtagcgtca acaccggcgg
     4801 ttgattgacc actgaacgcg tggccagcca accggcggca cagactaaca agcctccgcc
     4861 caccaggccg tagaaccaca cccacgggtc cgcggaatag ggaatctgca ggacctgtgt
     4921 cgcgatatat acaccggcaa tggacgcgcc ggcagcggcc agcgcgccag acaacacgcc
     4981 gagcgcggcg aactccgcca gcaacccctt gagaacggtg gagcgctgcg cgccgagcgt
     5041 tcgcagcatc gcgctttcat agcggcgctc ctcgcggctc gcctgcaccg cggcgatgag
     5101 caccaccagg cccgccatga gcgtgaacag gaagacgctt tgaaccgccg ccacggcctt
     5161 gtcgatgacc gaacgcacct ggcgcagaag atcgtccacg ttgaacaccg acacgctcgg
     5221 aaagcggcgc accaggtccg agatggtttt cggattggtg gccgtgagat gcgcagccgt
     5281 catccaggtg ccctgcgatc cgtccagcat gcccggcgcg aacatcagga agaagttggg
     5341 ctggaagctg tcccacttca cgtcgcgaac gctcgaaatt ttcgcggagt gcgtttcgcc
     5401 ggcaatgtcg aactgcagtt cgtcgccgag cttgaggttc atttcctcca tgtactcggt
     5461 ggacaccgag accagcggtt tgccgaaatc ctcgggcgtg aaccatttgc cgtcggtcac
     5521 cacggtgtcg ttgccgagtg tttccgccca cgtgatgttc tgatcgcggt tggcgaactc
     5581 ccggccgcgg cgcgtggcga acttgatgtc gtccaccggc gtgccgttga tctgcgtcat
     5641 gcgtgcccgg accatcggcc gcgcacgcgc gtgttgcgcg ccgcgttcct cgaggaacgt
     5701 ggtgaacgcc tggtgctcat tcggcggaat gttgatgaag aagaagttcg gcgtgtcggc
     5761 cggcagactg cggcgccagt cgttgagcag gtcgttgcgc accaccgcca gcaacagcag
     5821 catcatgatg ccgagcgcga atgccgtgag ctgcacgacg ctttccgtac gccgacgccc
     5881 gaggttcgcg ataccgtagc gccaggagac tcccacgcca ccgcggaacc gggtagttag
     5941 tttgacgagt gcccagccgg cgagcgccgt cacgagtacg aaccccgaaa gcccgatcac
     6001 gaacgcgaag aatgtcttct gatcgcgcag cacccagtag atgaggaatg cgatggcgag
     6061 gaccgctgga ccgaaggcga gcacgacgag cggctgcggt ggaccgacgt cgcgacgcag
     6121 tacgcgcaac gccggcgttc gcgacaactg cagcagcggc ggcagcgcga aaccgccaag
     6181 tacggctatg gcggtgagga agccgatgcc gagtggcgcg agcccgggcg cgggcagatc
     6241 gccgcgcagc aggcccgcga gcgccttgac gagccaggcc tgcgcgccga agcccagcag
     6301 cgaacccagc accgcggcga tcacgcccac catcgtgagc tggatcaggc tgacggccag
     6361 ggtgaatgcg cgcgttgcgc ccagcgtctt gagcagcgcg accgaatcga gatgccgatg
     6421 gacgtagcgc cttgccgcca tcgccaccgc gatcgcgcat aacaacaccg ccaccaggct
     6481 ggcgatgctg aggaatcggg ccgagcgatc gatggccgcc ttgatctgcg gagccgagtc
     6541 ctcgacgtcc tcgatctctt cgttcggctt cttgtttgcc tcgagccaga tcttgaacga
     6601 tgcgatttcg tcgcgctgcc ccgcgaaaag ctgcgcgcgc cgcaaccggc tgccgggttg
     6661 gacgagctgc gtggacgcca ggtcgtcctc gttcatgatg aggcttggcg ccagctcgag
     6721 aaacgtggcg ccctgatccg gacgcgtgat gaggatgcgg gagacacgga acgtgcccgt
     6781 gccgatgttg agatcggttc cgacggccgc tcccagcgcg gccgcgagcc gcgagtccgg
     6841 ccatacttcg ccgcgaccgg gattccccgt cgaaacctga ggctcgccga aaggctcgtc
     6901 ggagagcat
//
LOCUS       DATDEU010000755         4158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U208
            k141_1312889, whole genome shotgun sequence.
ACCESSION   DATDEU010000755 DATDEU010000000
VERSION     DATDEU010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962411
            Sequence Read Archive: SRR25158251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:35:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,588
            CDSs (total)                      :: 3,559
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312889"
                     /isolate="SMAG_U208"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="55.94 N 3.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VIV63_17925"
     CDS             <1..190
                     /locus_tag="VIV63_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6126538.1"
                     /translation="IIRGVVIVGAFTIAIQALRMDEDPRWKKSRLLPYAVGVAEALRG
                     IVGEQLERIDLPKSSVST"
     gene            204..1712
                     /gene="purF"
                     /locus_tag="VIV63_17930"
     CDS             204..1712
                     /gene="purF"
                     /locus_tag="VIV63_17930"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY6126539.1"
                     /translation="MCGIAGIVGTSGVNQRIYDALTVLQHRGQDAAGIATVQDGELFT
                     RKGRGLVRDVFEQHHMQELKGNIGMGHVRYPTAGCDSASEAQPFYVNAPYGICLAHNG
                     NLTNARELGEVLEREDRRHLNTSSDSEVLLNVLASELQRVGTPRVTPADIFAAVSAVY
                     RRCRGGYAVVAMVIGHGLLAFRDPNGIRPLVLGQRETPKGAEWMLASESVALDMLGFK
                     LVRDVAPGEVVYIDVHGRLHSQVCSATTKHAPCIFEYVYLARPDSLIDNISVHRARMR
                     MGERLAEKILRERAEHQIDVVIPIPDTSRTSAMQVAQRLDVKYREGFVKNRYVGRTFI
                     MPGQEQRSKSVRSKLNAIDLEFRNKNVLLVDDSIVRGTTSAQIIDLAREAGAKKVYFA
                     SAAPPVKFPNVYGIDMPAASELVAANRTEAEVAKLIGADWLIYQDLEDLIQACVHDHS
                     SIKEFDTSCFSGHYVTGDVTPEYLARLQSERSDMAKASRRDGEKSALRVVKT"
     gene            1743..2927
                     /locus_tag="VIV63_17935"
     CDS             1743..2927
                     /locus_tag="VIV63_17935"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEY6126540.1"
                     /translation="MNARRILLDVFDVALRAVDGRASVDRFLRETTLPSPVEVFAIGK
                     AASAMARGAQVALGPAIGRMLVITKDGHTDPTLHDTPGVTQVESAHPVPDLRSFSHGN
                     ELEERLRGLREDIFPLFLISGGSSSLVEALRDGVSLDEVLALNVQGLASGWDIARLNI
                     ARTRLSRIKGGGIARLLRGRRALALFISDVPGDDPDVIGSGLLGHDVGISDGIERHVI
                     ATIETAVHTVRDAARAHGLSLDAIATRFDGDADQVAGEVLARLRAGDSDGLVWGGEST
                     VTLPPQHGRGGRNTHLALSFARQLRAGEPWLLLAAGTDGTDGPTSDAGAIVDAGTVDR
                     ATVAGCDVERAWRDFDSGTALEAAEDLVHTGPTGTNVGDILIAIKQSASSLRGQPRPP
                     ML"
     gene            2936..>4158
                     /locus_tag="VIV63_17940"
     CDS             2936..>4158
                     /locus_tag="VIV63_17940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY6126541.1"
                     /translation="MRLMLIDDDPRYRTLLRHHISCAWPDVELVSYNPRVRGPLTPGF
                     LAQGYSVVLLDHEWKGGSGLDWLKDFHGREGFAPVIFLSAEDESPDAVEARATGAFEV
                     IGKTKIKHTKLNDAIRRAADEQAKAQSRWRMSAGAKMAQDFAGARLKEYRRIELIAKG
                     SVSELFLAESARDGDVVVLKVTPAIRKETGVDQSMERFLQEFEILREIQHPNIVRIYD
                     LGVTDDHLFLAMEHFARGDLRKRMSEGLTARQSLAYARDLAYALAAIHEVGIFHRDLK
                     PGNVMLRDDGSIALIDFGLAKHVALKMEVTDKGLIFGTPHYMSPEQGHGKEIDARSDL
                     YALGVMLYEMLTGKKPFDAENHMAILVHHAKAPIPKLPERIASLQPLIDTLMAKDPAN
                     RPAPAAIAATLIDYAL"
BASE COUNT          756 a         1386 c         1330 g          686 t
ORIGIN      
        1 catcatccgc ggcgtcgtca tcgtgggcgc gttcacgatc gccatccagg cgctgcgcat
       61 ggacgaagat ccccgctgga agaagtcccg cctgctgccg tatgcggtcg gtgtcgccga
      121 agctctgcgg ggcatcgtcg gcgagcaact cgaacgaatc gatctgccca aatcgtccgt
      181 ctccacctga ggatcagaac cgaatgtgcg gcatagccgg aatcgtcgga acgagcggag
      241 tcaatcaacg tatttacgac gccctgacgg tgctccagca ccgtgggcag gatgccgccg
      301 gcatcgccac cgtccaggac ggtgagctgt tcacgcgcaa gggccgcggc ctggtgcgcg
      361 acgtgttcga gcagcaccac atgcaggagc tcaagggcaa catcggcatg gggcacgtgc
      421 ggtacccgac ggccggctgc gacagcgcct cggaagccca gcctttctac gtgaacgcgc
      481 cgtacggcat ctgcctcgcg cacaacggca atctcaccaa cgcgcgcgaa ctcggcgaag
      541 tgctcgagcg cgaagaccgc cgccacctca acaccagttc cgattccgaa gtgctgctca
      601 acgtgctggc ttccgagctg cagcgcgtgg gcacgccgcg agtcacgccc gccgacatct
      661 tcgccgcggt gagcgcggtg tatcgccgct gccgtggtgg ctacgcggtg gtggccatgg
      721 tcatcggcca cggcctgctg gcgttccgcg atccgaatgg catccgcccg ctggtgctgg
      781 gtcagcgcga gacgccgaag ggcgcggaat ggatgctggc gtccgaaagc gtggcgctcg
      841 acatgctggg cttcaagctg gtgcgcgacg tggcgcccgg cgaagtcgtc tacatcgacg
      901 tgcatggacg cctgcattcg caggtgtgtt cggccaccac caaacacgcg ccctgcatct
      961 tcgaatacgt ttacctcgcc cgacccgact cgctgatcga caacatctcg gtgcatcgcg
     1021 cgcgcatgcg catgggcgaa agactcgccg agaagatcct gcgcgagcgc gccgagcacc
     1081 agatcgacgt cgtcatcccg attcccgaca ccagccgcac cagcgccatg caggtcgcgc
     1141 agcgactcga cgtgaagtac cgcgaagggt tcgtgaagaa ccgctacgtg ggccgcacgt
     1201 tcatcatgcc gggccaggag cagcgttcga agtcggtacg cagcaagctc aacgccatcg
     1261 atctcgagtt ccgcaacaag aacgtcctgc tggtcgacga ctccatcgtg cgcggcacca
     1321 cctccgcgca gatcatcgac ctcgctcgcg aggccggtgc gaaaaaggtc tatttcgcct
     1381 cggccgcgcc gccggtgaaa ttcccgaacg tctacggcat cgacatgccg gcggccagcg
     1441 aactcgtcgc cgccaatcgc accgaggccg aagtggccaa actcatcggc gcggactggc
     1501 tgatctacca ggatctcgaa gatctcatcc aggcatgtgt tcacgatcat tccagcatca
     1561 aggaattcga cacttcctgt ttctcgggac actacgtcac cggtgacgtg actcccgaat
     1621 accttgcgcg cctgcagtcg gaacgttccg atatggccaa ggcgtcgaga cgtgacggtg
     1681 aaaagtccgc tctcagggtc gtcaagacct gatcggtccg cgggctcgcc gggcccgccg
     1741 ccatgaacgc gcgtcgtatc ctgctcgatg tattcgacgt cgcgctgcgt gcagtcgacg
     1801 gtcgcgccag cgtcgaccgc ttcctgcgcg aaaccacgct gccttcgccc gtcgaagtgt
     1861 tcgccatcgg aaaggctgcg agtgccatgg cgcgcggcgc gcaggtcgcg ctggggccgg
     1921 cgatcggtcg aatgctggtc atcacgaagg atggccacac cgacccgaca cttcacgaca
     1981 cgcccggcgt cacgcaggtg gagagcgcac atccggtgcc cgacctgcgc agcttcagcc
     2041 atggcaatga actcgaggag cgcctgcgcg gcctgcgcga agacatcttc ccgttgttcc
     2101 tgatctcggg cggaagctcg agtctcgtgg aagcgctgcg tgacggcgtt tcgctcgacg
     2161 aagtgctcgc gctcaacgtg cagggtctcg cgagcggctg ggatatcgcc cggctgaata
     2221 ttgcgcgcac gcgcctgtcg cgcatcaagg gtggtggcat cgcgcgcctg ctgcgcggcc
     2281 gccgcgcgct ggcgctgttc atctcggacg tgcccggcga cgatccggac gtgatcggtt
     2341 cggggttgtt agggcacgac gtcggcatat ccgacggcat cgaaaggcac gtgatcgcca
     2401 ccatcgaaac cgcggtgcac acggtccgcg acgcggcgcg cgcccacgga ctttccctcg
     2461 atgcgatcgc cacgcgattc gacggcgacg cggaccaggt ggcgggtgaa gtgctcgcgc
     2521 gattgcgtgc cggcgattcc gacgggctcg tgtggggtgg cgagtcgacc gtgacactgc
     2581 cgccccaaca cggtcgcggc ggccggaaca cgcatctggc gttgtcgttc gcgcggcagt
     2641 tgcgcgccgg tgagccgtgg ctgttgctgg ccgcgggcac cgatgggacc gacggtccga
     2701 ccagcgatgc gggcgccatc gtcgacgcgg gaaccgtcga tcgcgcaacc gttgcgggct
     2761 gcgacgtcga gcgcgcgtgg cgcgatttcg attccggcac ggcgctcgaa gctgcggaag
     2821 atctcgtgca cacggggcca acgggcacga atgtcgggga tatcctgatc gccatcaaac
     2881 aatccgcaag ctcgttgcgt ggccaacccc ggccacccat gttgtaatcc gcgcgatgcg
     2941 cttgatgctg atcgacgatg acccgcggta ccgcacgctg ctgcgtcacc acatctcctg
     3001 tgcctggccc gacgtggagc tggtgagcta caacccgcgt gtgcgcggac cgctgacacc
     3061 gggttttctc gcgcagggct acagcgtcgt gttgctcgac cacgaatgga agggcggttc
     3121 cggcctcgac tggctcaagg actttcacgg ccgcgaaggt tttgcaccgg tcatcttcct
     3181 gtcggccgag gacgaatccc ccgacgcggt ggaagcacgc gcgacgggtg cgttcgaagt
     3241 catcggcaag accaagatca agcacaccaa gctgaacgac gcgatccgcc gtgccgccga
     3301 cgagcaggcc aaggcgcaga gccggtggcg catgtcggcc ggcgcgaaga tggcgcagga
     3361 cttcgccggt gcgaggctca aggaataccg gcgcatcgaa ctcatcgcca agggttccgt
     3421 gtcagagctg tttctcgccg agagcgcacg cgacggagac gtagtggtgc tcaaggtcac
     3481 gcccgccatc cgcaaggaaa ccggcgtcga ccaatcgatg gagcgcttcc tgcaggaatt
     3541 cgagattttg cgcgagatcc agcatcccaa catcgtgcgc atctacgatc ttggcgtcac
     3601 cgacgaccac ctgttcctgg cgatggaaca ctttgcgcgc ggcgacctgc gcaagcgcat
     3661 gtccgagggc ctgaccgcgc ggcaatcgct ggcctatgcg cgcgaccttg cctacgcgct
     3721 ggcggccatt cacgaggtcg gcatttttca tcgcgacctc aagcccggca acgtgatgct
     3781 gcgcgacgac ggcagcatcg cgctcatcga cttcgggctc gccaagcacg tggcgctcaa
     3841 gatggaggtt accgacaagg gcctgatctt cggcacgccg cactacatga gcccggagca
     3901 gggccacggc aaggagatcg acgcgcgcag cgatctgtat gcgctcggcg tgatgctcta
     3961 cgaaatgctg acgggcaaga aaccgttcga cgccgaaaac cacatggcaa ttctcgtcca
     4021 tcacgccaag gcgccgatcc ccaaactacc cgaacggatc gcgtcgctgc agccgctcat
     4081 cgacaccttg atggcgaagg atcccgcgaa ccgtccggcc cccgccgcga tcgccgcgac
     4141 gctcatcgac tacgcgct
//
